# A Novel Method for High-Level Production of TEV Protease by Superfolder GFP Tag

Authors: Xudong Wu; Di Wu; Zhisheng Lu; Wentao Chen; Xiaojian Hu; Yu Ding
Journal: Journal of Biomedicine and Biotechnology
 (2009)

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## Abstract

Because of its stringent sequence specificity, tobacco etch virus (TEV) protease is widely used to remove fusion tags from recombinant proteins. Due to the poor solubility of TEV protease, many strategies have been employed to increase the expression level of this enzyme. In our work, we introduced a novel method to produce TEV protease by using visible superfolder green fluorescent protein (sfGFP) as the fusion tag. The soluble production and catalytic activity of six variants of sfGFP-TEV was examined, and then the best variant was selected for large-scale production. After purified by Ni-NTA affinity chromatography and Q anion exchange chromatography, the best variant of sfGFP-TEV fusion protease was obtained with purity of over 98% and yield of over 320 mg per liter culture. The sfGFP-TEV had a similar catalytic activity to that of the original TEV protease. Our research showed a novel method of large-scale production of visible and functional TEV protease for structural genomics research and other applications.


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## Body

## 1. Introduction

Nowadays, it has been a popular way to fuse target proteins with various tags to facilitate expression and purification. An efficient combination of solubility-enhancing tags, such as maltose-binding protein (MBP) [1, 2], N-Utilization substance (NusA) [3], glutathione S-transferase (GST) [4], thioredoxin (TRX) [5], trigger factor [6], and SUMO [7], will promise high-throughput expression and purification methods for many target proteins and sometimes increases their solubility. However, these fusion tags may become a drawback for further structural and functional studies [8]. Therefore, the removal of these tags is necessary in many situations. Proteases such as enterokinase, thrombin, and factor Xa [9] as well as the more specific human rhinovirus 3C protease (3CP or PreScission [10]) and tobacco etch virus (TEV) protease [11] can fulfill the task to liberate fusion tags from target proteins.The widely used TEV protease is the 27 kDa catalytic domain of the nuclear inclusion an (NIa) protease from tobacco etch virus [12]. Among various proteases, TEV protease outstands because of its high and unique specificity. It can recognize the canonical cleavage site, ENLYFQ/G [11] and the P1’ position can tolerate substitutions with small amino acids [13]. Moreover, TEV protease can be used at temperature as low as 4°C with adequate efficiency to reduce the proteolysis of the target protein. Because of these advantages, nowadays, it is used more frequently than other proteases (enterokinase, thrombin, factor Xa, and human rhinovirus 3C protease) in structural genomic research projects.Production of TEV protease inE. coli has been problematic due to its low solubility. To increase its soluble production, many strategies have been addressed. First, Kapust et al. [14] designed a more stable mutant of TEV protease named S219V. van den Berg et al. [15] obtained a mutant TEVSH with production of 54 mg/L culture by directed evolution. Later, Fang et al. [16] increased the production to 65 mg/L culture using chaperone coexpression and low-temperature expression methods. More recently, Blommel and Fox [17] reported a combined approach raising the production to 400 mg/L culture while Kraft et al. developed a fluorogenic substrate which was useful to determine the TEV protease's expression and folding in vivo [18].Fluorescent protein is widely used as gene reporter and protein marker, and so forth. However, existing variants of green fluorescent protein (GFP) often misfold when fused to other proteins. Pédelacq et al. [19] reported a robustly folded GFP called “superfolder GFP” (sfGFP) which could fold well regardless of the folding status or solubility of its fusion partner in E. coli. Furthermore, sfGFP fusions are more soluble than conventional GFP fusions.In our present work, considering the high thermodynamic stability, robust folding kinetics, and solubility ofsfGFP fusions, we tempted to fuse sfGFP to TEV protease hoping that sfGFP would increase the soluble production of TEV protease. In order to minimize the possible stereo-hindrance of sfGFP that might decrease the activity of TEV protease, we further constructed 6 variants of sfGFP-TEV with different linkers of various lengths and composition between sfGFP and TEV. Then, the catalytic activity of sfGFP-TEV variants was tested and compared with that of the original TEV protease without sfGFP tag. Finally, we obtained one variant of sfGFP-TEV fusion protease with soluble production of over 320 mg/L culture. Compared with the original TEV protease, this variant of sfGFP-TEV has similar catalytic activity and is easy for detection during expression, purification, and applications because of the presence of green fluorescence. The results of our work also present the potential of superfolder GFP to become a solubility-enhancing fusion tag with fluorescence.


## 2. Materials and Methods

### 2.1. Materials

The bacterial hosts,E. coliDH5α, Rosetta (DE3) pLysS, and the vector pET21a were obtained from Novagen (Madison, WI). KOD Plus polymerase and the DNA ligation kit were purchased from Toyobo (Osaka, Japan). Nucleotides, agarose gel, the DNA extraction kit, and the PCR purification kit were purchased from Roche Diagnostics (Indianapolis, IN). Primer synthesis and DNA sequence analysis were performed by Invitrogen (Shanghai, China). Restriction endonucleases were purchased from Takara (Dalian, China). The nickel-nitrilotriacetic acid (Ni-NTA) superflow matrix was obtained from Qiagen (Chatsworth, CA). Q Sepharose Fast Flow was from GE Health (Sweden). Amylose Sepharose was purchased from New England Biolabs (Hitchin, UK). Bicinchoninic acid (BCA) Protein Assay Reagent Kit was from Pierce (Rockford, IL). Imidazole, D-glucose, and D-lactose were from Sigma (St Louis, MO). All other agents are of analytic purity. PRK793-TEV expression vector was a gift from Dr. Waugh [14].


### 2.2. Construction of sfGFP-TEV and TEV Expression Vectors

We have previously reconstructed an expression vector, designated pT7His, which contained the N-terminal His10 and C-terminal His6 tags from the vector pET21a. The detailed vector construction procedure was similar to that of pT7470 with N-terminal His6 and C-terminal His6 tags [20]. We optimized the codon usage of superfolder GFP’s cDNA by referring to its amino acid sequence [19]. The whole gene synthesis of superfolder GFP was accomplished by 2 rounds PCR with 18 central primers listed in Table 1, one 5′ primer 5′-GATATACATATGAGCAAAGGCGAAGAA-3′ and one 3′ primer 5′-GCCGGATCCGCCCCCGGAACCCCCTCCGTTATTGTTATTCTTGTACAGCTCGTCCAT-3′. Considering that the C-terminal poly (R) in PRK793-TEV would decrease the solubility of TEV protease [17], we replaced the poly (R) with residue E to construct the plasmid TEV 238Δ by PCR with primers 5′-GGGGGTAGCGGCGGTGGCAGCGGCGGAGAAAGCTTGTTTAAG-3′ and 5′-TTACTCGAGTCATTCATTCATGAGTTGAGTCGC-3′. We have constructed 6 recombinant sfGFP-TEV fusion proteins with different linkers. The linker region of sfGFP-TEV-His6 Nd1–6 was listed in Table 2. The plasmid TEV 238Δ was also used as the PCR template to produce the control TEV protease. The PCR product was incorporated into the expression vector MBP-LTL-His6 [21]. The final expression vector MBP-LTL-TEV-His6 which produced TEV-His6 (MBP tag was self-cleaved during expression) was employed as a control in further experiments.Table 1
Primers for whole gene synthesis of superfolder GFP.
No. and lengthOligo Sequences (5′→3′)S1F 59ATGAGCAAAGGCGAAGAACTGTTTACCGGCGTGGTGCCGATTCTGGTTGAACTGGATGGS2R 59GCCTTCGCCGCGCACGCTAAACTTATGGCCATTAACATCGCCATCCAGTTCAACCAGAAS3F 59TAGCGTGCGCGGCGAAGGCGAAGGCGATGCGACCAACGGCAAACTGACCCTGAAGTTTAS4R 59AGGGTCGGCCACGGCACCGGCAGTTTGCCGGTGGTGCAAATAAACTTCAGGGTCAGTTTS5F 59CGGTGCCGTGGCCGACCCTGGTGACCACCCTGACCTATGGCGTGCAGTGCTTTAGCCGCS6R 59CGGACTTAAAGAAATCATGGCGTTTCATGTGATCCGGATAGCGGCTAAAGCACTGCACGS7F 59CATGATTTCTTTAAGTCCGCGATGCCGGAAGGCTATGTTCAGGAACGCACCATTAGCTTS8R 59TTTCACTTCCGCGCGGGTCTTATAGGTGCCATCATCTTTAAAGCTAATGGTGCGTTCCTS9F 59GACCCGCGCGGAAGTGAAATTTGAAGGCGATACCCTGGTGAACCGCATTGAACTGAAAGS10R 59TTATGGCCCAGAATGTTGCCATCTTCTTTGAAGTCAATGCCTTTCAGTTCAATGCGGTTS11F 59GCAACATTCTGGGCCATAAACTGGAGTACAATTTCAACAGCCATAACGTGTATATTACCS12R 59TTTTGAAATTCGCTTTGATGCCGTTCTTCTGTTTGTCCGCGGTAATATACACGTTATGGS13F 59ATCAAAGCGAATTTCAAAATCCGCCATAATGTGGAAGATGGCAGCGTGCAGCTGGCGGAS14R 59CACCGGGCCATCGCCGATCGGGGTATTCTGCTGATAGTGATCCGCCAGCTGCACGCTGCS15F 59GATCGGCGATGGCCCGGTGCTGCTGCCGGACAATCACTACCTGAGCACCCAGTCCGTGCS16R 54CATGTGATCGCGTTTCTCATTCGGATCTTTGCTCAGCACGGACTGGGTGCTCAGS17F 54TGAGAAACGCGATCACATGGTTCTGCTGGAGTTTGTGACCGCGGCGGGTATCACS18R 44CTTGTACAGCTCGTCCATGCCATGAGTGATACCCGCCGCGGTCATable 2
Linker region ofsfGFP-TEV-His6 Nd1–6.
Construction CodeAbbreviationLinker length (aa)Anticipated linker compositionSfGFP-TEV-His6 Nd1Nd12…THG/GS/RD…SfGFP-TEV-His6 Nd2Nd25…THG/GSKGP/RD…SfGFP-TEV-His6 Nd3Nd38…THG/NNPGSKGP/RD…SfGFP-TEV-His6 Nd6Nd68…THG/GSNLFKGP/RD…SfGFP-TEV-His6 Nd4Nd411…THG/NNPGSNLFKGP/RD…SfGFP-TEV-His6 Nd5Nd514…THG/MDPNNPGSNLFKGP/RD…


### 2.3. Expression of sfGFP-TEV, TEV-His6, and MBP-EGFP

The expression vectors mentioned above were transformed intoE. coli strain Rosetta (DE3) pLysS. After the colony had grown overnight at 37°C in 5 mL of LB medium with 100 μg/mL ampicillin, 0.5 mL of the bacterial suspension was transferred into a 2L flask containing 250 mL autoinduction medium. (For 1 liter culture, we used 4 flasks to ensure the sufficient oxygen supply). The autoinduction medium was prepared as studier’s original protocol [22]. Standard stock solutions include 20*P (1 M Na2HPO4, 1 M KH2PO4, and 0.5 M (NH4)2SO4), 50*M (1.25 M Na2HPO4, 1.25 M KH2PO4, 2.5 M NH4Cl, and 0.25 M Na2SO4), and 50*5052 (25% glycerol, 2.5% glucose, and 10% D-lactose); the working autoinduction medium was assembled by adding sterile concentrated stock solutions into sterile water. When the cells had grown (250 rpm) at 37°C to an optical density at 600 nm (OD600) of 0.6 (around 3 hours), the cells were cooled to 19°C and shaken at 250 rpm for 20 hours. Finally, the cells were collected by centrifugation at 6,000 ×g for 20 minutes and stored at -80°C. In order to reflect the real-time expression level of sfGFP-TEV, the induced E. coli cells in the autoinduction medium were collected at 0, 2, 4, 6, 8, 10, 12, 14, and 16 hours, respectively. The fluorescence of 100 μL E. coli cells in the 96-well plates was recorded by DTX 880 multimode detector (Beckman) using bottom reading method with 485 nm excitation filter and 535 nm emission filter.


### 2.4. Purification of sfGFP-TEV and TEV-His6

ThesfGFP-TEV-His6 Nd1–6 recombinant proteins were all first purified by Ni-NTA affinity chromatography. The frozen cell pellet was thawed and resuspended in Buffer A (50 mM Tris–HCl [pH 8.0], 150 mM NaCl, 10% [v/v] glycerol, 20 mM imidazole). Then, the cells were lysed by sonication on ice and the lysate was cleared by two-round 20-minute centrifugation at 20,000 ×g. The retained supernatant was loaded onto a Ni-NTA Superflow column which was pre-equilibrated with Buffer A. After loading, the Ni-NTA column was washed with Buffer A with 40 mM imidazole. The column was equilibrated again with Buffer A and then eluted with Buffer B (50 mM Tris–HCl [pH 8.0], 150 mM NaCl, 10% [v/v] glycerol, 500 mM imidazole). The fluorescence of 100 μL Ni-NTA purification sample was also recorded by DTX 880 multimode detector (Beckman) using top reading method with 485 nm excitation filter and 535 nm emission filter.The collected elution from Ni-NTA affinity chromatography was immediately diluted with 5 volume QA (50 mM Tris–HCl [pH 8.0], 10% [v/v] glycerol). The dilution was loaded onto a Q Sepharose Fast Flow column pre-equilibrated with QA. After washed with QA, the protein was eluted with a linear 0–0.9 M NaCl gradient by automatically mixing QA and QB (50 mM Tris–HCl [pH 8.0], 1 M NaCl, 10% [v/v] glycerol). Fractions were analyzed by SDS-PAGE and quantified by BandScan 4.30 (Glyko) and were pooled based on their purity and concentration. The concentration of pooled protein sample from Q anion exchange chromatography and elution sample from Ni-NTA affinity chromatography was determined by BCA method according to the reagent kit protocol (Pierce). The pooled protein was dialyzed in dialysis buffer (50 mM Tris–HCl [pH 8.0], 150 mM NaCl, 0.5 mM EDTA, 10% [v/v] glycerol) at4°C and then diluted with storage buffer (50 mM Tris–HCl [pH 8.0], 150 mM NaCl, 0.5 mM EDTA, 80% [v/v] glycerol) to a protein concentration of ~2 mg/mL in 40% glycerol. The purified protein was finally stored at -20°C.TEV-His6 was purified by Ni-NTA affinity chromatography using the similar methods described above. The purified protein was dialyzed in dialysis buffer and diluted with storage buffer to a protein concentration of ~1 mg/mL in 40% glycerol. The purified TEV-His6 was stored at -20°C.


### 2.5. Purification of TEV Protease Substrate MBP-EGFP

For the purification of MBP-EGFP, the cell pellet was thawed and resuspended in Amylose A buffer (50 mM Tris–HCl [pH 8.0], 150 mM NaCl, 10% [v/v] glycerol). After sonication, the supernatant was retained by two-round 20-minute centrifugation at 20,000×g and then loaded onto the Amylose Sepharose Column pre-equilibrated with Amylose A buffer. MBP-EGFP was eluted with Amylose B buffer (50 mM Tris–HCl [pH 8.0], 150 mM NaCl, 10% [v/v] glycerol, 20 mM maltose). The purified protein sample was analyzed by SDS-PAGE and quantified by BandScan 4.30 (Glyko). The purified MBP-EGFP was dialyzed in dialysis buffer (150 mM NaCl, 10% [v/v] glycerol, 1 mM EDTA) at 4°C and then stored at 4°C.


### 2.6. Activity Assay of sfGFP-TEV and TEV-His6

The catalytic activity ofsfGFP-TEV-His6 Nd1–6 and TEV-His6 was determined by cleaving the substrate MBP-EGFP which contained a TEV cleavage site between MBP and EGFP. Prior to activity assay, the protein concentration of sfGFP-TEV-His6 Nd1–6, TEV-His6, and MBP-EGFP was determined by BCA method according to the reagent kit protocol (Pierce). The time course assay was conducted at 17°C for a given incubation time (0, 5, 10, 20, 40, 60, 90, 120, 180, and 240 minutes, respectively). The mass ratio of substrate to enzyme (calculated by the mass of effective TEV protease) is 100:1. At any given time, the reaction was stopped by adding 3×loading buffer (150 mM Tris-HCl [pH 6.8], 300 mM DTT, 6% [w/v] SDS, 0.06% [w/v] bromophenol blue, 30% [v/v] glycerol). The samples were boiled at 95°C for 3 minutes and then loaded onto 12% SDS-PAGE gel for electrophoresis. After visualized by staining with Coomassie G-250, the gel was quantified by BandScan 4.30 (Glyko) to establish the time-course curve. The reaction condition was 75 mM NaCl, 0.5 mM EDTA, 25 mM Tris-HCl 8.0, and 10% [v/v] glycerol.


## 2.1. Materials

The bacterial hosts,E. coliDH5α, Rosetta (DE3) pLysS, and the vector pET21a were obtained from Novagen (Madison, WI). KOD Plus polymerase and the DNA ligation kit were purchased from Toyobo (Osaka, Japan). Nucleotides, agarose gel, the DNA extraction kit, and the PCR purification kit were purchased from Roche Diagnostics (Indianapolis, IN). Primer synthesis and DNA sequence analysis were performed by Invitrogen (Shanghai, China). Restriction endonucleases were purchased from Takara (Dalian, China). The nickel-nitrilotriacetic acid (Ni-NTA) superflow matrix was obtained from Qiagen (Chatsworth, CA). Q Sepharose Fast Flow was from GE Health (Sweden). Amylose Sepharose was purchased from New England Biolabs (Hitchin, UK). Bicinchoninic acid (BCA) Protein Assay Reagent Kit was from Pierce (Rockford, IL). Imidazole, D-glucose, and D-lactose were from Sigma (St Louis, MO). All other agents are of analytic purity. PRK793-TEV expression vector was a gift from Dr. Waugh [14].


## 2.2. Construction of sfGFP-TEV and TEV Expression Vectors

We have previously reconstructed an expression vector, designated pT7His, which contained the N-terminal His10 and C-terminal His6 tags from the vector pET21a. The detailed vector construction procedure was similar to that of pT7470 with N-terminal His6 and C-terminal His6 tags [20]. We optimized the codon usage of superfolder GFP’s cDNA by referring to its amino acid sequence [19]. The whole gene synthesis of superfolder GFP was accomplished by 2 rounds PCR with 18 central primers listed in Table 1, one 5′ primer 5′-GATATACATATGAGCAAAGGCGAAGAA-3′ and one 3′ primer 5′-GCCGGATCCGCCCCCGGAACCCCCTCCGTTATTGTTATTCTTGTACAGCTCGTCCAT-3′. Considering that the C-terminal poly (R) in PRK793-TEV would decrease the solubility of TEV protease [17], we replaced the poly (R) with residue E to construct the plasmid TEV 238Δ by PCR with primers 5′-GGGGGTAGCGGCGGTGGCAGCGGCGGAGAAAGCTTGTTTAAG-3′ and 5′-TTACTCGAGTCATTCATTCATGAGTTGAGTCGC-3′. We have constructed 6 recombinant sfGFP-TEV fusion proteins with different linkers. The linker region of sfGFP-TEV-His6 Nd1–6 was listed in Table 2. The plasmid TEV 238Δ was also used as the PCR template to produce the control TEV protease. The PCR product was incorporated into the expression vector MBP-LTL-His6 [21]. The final expression vector MBP-LTL-TEV-His6 which produced TEV-His6 (MBP tag was self-cleaved during expression) was employed as a control in further experiments.Table 1
Primers for whole gene synthesis of superfolder GFP.
No. and lengthOligo Sequences (5′→3′)S1F 59ATGAGCAAAGGCGAAGAACTGTTTACCGGCGTGGTGCCGATTCTGGTTGAACTGGATGGS2R 59GCCTTCGCCGCGCACGCTAAACTTATGGCCATTAACATCGCCATCCAGTTCAACCAGAAS3F 59TAGCGTGCGCGGCGAAGGCGAAGGCGATGCGACCAACGGCAAACTGACCCTGAAGTTTAS4R 59AGGGTCGGCCACGGCACCGGCAGTTTGCCGGTGGTGCAAATAAACTTCAGGGTCAGTTTS5F 59CGGTGCCGTGGCCGACCCTGGTGACCACCCTGACCTATGGCGTGCAGTGCTTTAGCCGCS6R 59CGGACTTAAAGAAATCATGGCGTTTCATGTGATCCGGATAGCGGCTAAAGCACTGCACGS7F 59CATGATTTCTTTAAGTCCGCGATGCCGGAAGGCTATGTTCAGGAACGCACCATTAGCTTS8R 59TTTCACTTCCGCGCGGGTCTTATAGGTGCCATCATCTTTAAAGCTAATGGTGCGTTCCTS9F 59GACCCGCGCGGAAGTGAAATTTGAAGGCGATACCCTGGTGAACCGCATTGAACTGAAAGS10R 59TTATGGCCCAGAATGTTGCCATCTTCTTTGAAGTCAATGCCTTTCAGTTCAATGCGGTTS11F 59GCAACATTCTGGGCCATAAACTGGAGTACAATTTCAACAGCCATAACGTGTATATTACCS12R 59TTTTGAAATTCGCTTTGATGCCGTTCTTCTGTTTGTCCGCGGTAATATACACGTTATGGS13F 59ATCAAAGCGAATTTCAAAATCCGCCATAATGTGGAAGATGGCAGCGTGCAGCTGGCGGAS14R 59CACCGGGCCATCGCCGATCGGGGTATTCTGCTGATAGTGATCCGCCAGCTGCACGCTGCS15F 59GATCGGCGATGGCCCGGTGCTGCTGCCGGACAATCACTACCTGAGCACCCAGTCCGTGCS16R 54CATGTGATCGCGTTTCTCATTCGGATCTTTGCTCAGCACGGACTGGGTGCTCAGS17F 54TGAGAAACGCGATCACATGGTTCTGCTGGAGTTTGTGACCGCGGCGGGTATCACS18R 44CTTGTACAGCTCGTCCATGCCATGAGTGATACCCGCCGCGGTCATable 2
Linker region ofsfGFP-TEV-His6 Nd1–6.
Construction CodeAbbreviationLinker length (aa)Anticipated linker compositionSfGFP-TEV-His6 Nd1Nd12…THG/GS/RD…SfGFP-TEV-His6 Nd2Nd25…THG/GSKGP/RD…SfGFP-TEV-His6 Nd3Nd38…THG/NNPGSKGP/RD…SfGFP-TEV-His6 Nd6Nd68…THG/GSNLFKGP/RD…SfGFP-TEV-His6 Nd4Nd411…THG/NNPGSNLFKGP/RD…SfGFP-TEV-His6 Nd5Nd514…THG/MDPNNPGSNLFKGP/RD…


## 2.3. Expression of sfGFP-TEV, TEV-His6, and MBP-EGFP

The expression vectors mentioned above were transformed intoE. coli strain Rosetta (DE3) pLysS. After the colony had grown overnight at 37°C in 5 mL of LB medium with 100 μg/mL ampicillin, 0.5 mL of the bacterial suspension was transferred into a 2L flask containing 250 mL autoinduction medium. (For 1 liter culture, we used 4 flasks to ensure the sufficient oxygen supply). The autoinduction medium was prepared as studier’s original protocol [22]. Standard stock solutions include 20*P (1 M Na2HPO4, 1 M KH2PO4, and 0.5 M (NH4)2SO4), 50*M (1.25 M Na2HPO4, 1.25 M KH2PO4, 2.5 M NH4Cl, and 0.25 M Na2SO4), and 50*5052 (25% glycerol, 2.5% glucose, and 10% D-lactose); the working autoinduction medium was assembled by adding sterile concentrated stock solutions into sterile water. When the cells had grown (250 rpm) at 37°C to an optical density at 600 nm (OD600) of 0.6 (around 3 hours), the cells were cooled to 19°C and shaken at 250 rpm for 20 hours. Finally, the cells were collected by centrifugation at 6,000 ×g for 20 minutes and stored at -80°C. In order to reflect the real-time expression level of sfGFP-TEV, the induced E. coli cells in the autoinduction medium were collected at 0, 2, 4, 6, 8, 10, 12, 14, and 16 hours, respectively. The fluorescence of 100 μL E. coli cells in the 96-well plates was recorded by DTX 880 multimode detector (Beckman) using bottom reading method with 485 nm excitation filter and 535 nm emission filter.


## 2.4. Purification of sfGFP-TEV and TEV-His6

ThesfGFP-TEV-His6 Nd1–6 recombinant proteins were all first purified by Ni-NTA affinity chromatography. The frozen cell pellet was thawed and resuspended in Buffer A (50 mM Tris–HCl [pH 8.0], 150 mM NaCl, 10% [v/v] glycerol, 20 mM imidazole). Then, the cells were lysed by sonication on ice and the lysate was cleared by two-round 20-minute centrifugation at 20,000 ×g. The retained supernatant was loaded onto a Ni-NTA Superflow column which was pre-equilibrated with Buffer A. After loading, the Ni-NTA column was washed with Buffer A with 40 mM imidazole. The column was equilibrated again with Buffer A and then eluted with Buffer B (50 mM Tris–HCl [pH 8.0], 150 mM NaCl, 10% [v/v] glycerol, 500 mM imidazole). The fluorescence of 100 μL Ni-NTA purification sample was also recorded by DTX 880 multimode detector (Beckman) using top reading method with 485 nm excitation filter and 535 nm emission filter.The collected elution from Ni-NTA affinity chromatography was immediately diluted with 5 volume QA (50 mM Tris–HCl [pH 8.0], 10% [v/v] glycerol). The dilution was loaded onto a Q Sepharose Fast Flow column pre-equilibrated with QA. After washed with QA, the protein was eluted with a linear 0–0.9 M NaCl gradient by automatically mixing QA and QB (50 mM Tris–HCl [pH 8.0], 1 M NaCl, 10% [v/v] glycerol). Fractions were analyzed by SDS-PAGE and quantified by BandScan 4.30 (Glyko) and were pooled based on their purity and concentration. The concentration of pooled protein sample from Q anion exchange chromatography and elution sample from Ni-NTA affinity chromatography was determined by BCA method according to the reagent kit protocol (Pierce). The pooled protein was dialyzed in dialysis buffer (50 mM Tris–HCl [pH 8.0], 150 mM NaCl, 0.5 mM EDTA, 10% [v/v] glycerol) at4°C and then diluted with storage buffer (50 mM Tris–HCl [pH 8.0], 150 mM NaCl, 0.5 mM EDTA, 80% [v/v] glycerol) to a protein concentration of ~2 mg/mL in 40% glycerol. The purified protein was finally stored at -20°C.TEV-His6 was purified by Ni-NTA affinity chromatography using the similar methods described above. The purified protein was dialyzed in dialysis buffer and diluted with storage buffer to a protein concentration of ~1 mg/mL in 40% glycerol. The purified TEV-His6 was stored at -20°C.


## 2.5. Purification of TEV Protease Substrate MBP-EGFP

For the purification of MBP-EGFP, the cell pellet was thawed and resuspended in Amylose A buffer (50 mM Tris–HCl [pH 8.0], 150 mM NaCl, 10% [v/v] glycerol). After sonication, the supernatant was retained by two-round 20-minute centrifugation at 20,000×g and then loaded onto the Amylose Sepharose Column pre-equilibrated with Amylose A buffer. MBP-EGFP was eluted with Amylose B buffer (50 mM Tris–HCl [pH 8.0], 150 mM NaCl, 10% [v/v] glycerol, 20 mM maltose). The purified protein sample was analyzed by SDS-PAGE and quantified by BandScan 4.30 (Glyko). The purified MBP-EGFP was dialyzed in dialysis buffer (150 mM NaCl, 10% [v/v] glycerol, 1 mM EDTA) at 4°C and then stored at 4°C.


## 2.6. Activity Assay of sfGFP-TEV and TEV-His6

The catalytic activity ofsfGFP-TEV-His6 Nd1–6 and TEV-His6 was determined by cleaving the substrate MBP-EGFP which contained a TEV cleavage site between MBP and EGFP. Prior to activity assay, the protein concentration of sfGFP-TEV-His6 Nd1–6, TEV-His6, and MBP-EGFP was determined by BCA method according to the reagent kit protocol (Pierce). The time course assay was conducted at 17°C for a given incubation time (0, 5, 10, 20, 40, 60, 90, 120, 180, and 240 minutes, respectively). The mass ratio of substrate to enzyme (calculated by the mass of effective TEV protease) is 100:1. At any given time, the reaction was stopped by adding 3×loading buffer (150 mM Tris-HCl [pH 6.8], 300 mM DTT, 6% [w/v] SDS, 0.06% [w/v] bromophenol blue, 30% [v/v] glycerol). The samples were boiled at 95°C for 3 minutes and then loaded onto 12% SDS-PAGE gel for electrophoresis. After visualized by staining with Coomassie G-250, the gel was quantified by BandScan 4.30 (Glyko) to establish the time-course curve. The reaction condition was 75 mM NaCl, 0.5 mM EDTA, 25 mM Tris-HCl 8.0, and 10% [v/v] glycerol.


## 3. Results and Discussion

### 3.1. Construction of Expression Vector for sfGFP-TEV and TEV

In order to maximize the expression level of the recombinantsfGFP-TEV proteases, we first synthesized the sfGFP gene according to the synonymous codon choice which is optimal for the Escherichia coli translational system. Figure 1 shows the vector map we used for high-level expression of sfGFP-TEV-His6 Nd1–6. The sfGFP-TEV coding sequence was cloned to the pET derived vector pT7His which possesses the strong bacteriophage T7 promoter, ensuring the high level expression of target protein. Considering that the linker between sfGFP and TEV might have effects on the stability and catalytic activity of fusion protease, we constructed 6 variants of sfGFP-TEV-His6 with different linkers. The linker here is defined as the peptide between C-terminus of sfGFP “THG” and N-terminus of TEV “RDYNP.” The composition of different linkers with lengths varying from 2 to 14aa could be referred to Table 2. We also incorporated a small peptide “GGG” at the C-terminus of TEV; so the C-terminuses of sfGFP-TEV-His6 Nd1–6 and TEV-His6 are all “LMNEGGGLEHHHHHH.” Our first attempt of sfGFP-TEV vector construction did not include the GGG small peptide between TEV and C-terminus His6 tag. However, during the Ni-NTA purification step, more than 70% expressed fusion protein did not bind with the Ni-NTA resin (data not shown). Perhaps the steric structure of TEV hindered His6 tag from binding with Ni-NTA resin. So we added the flexible GGG peptide between TEV and His6 tag. Almost all of the new version fusion protein can bind with Ni-NTA in the buffer containing relatively high concentration (20 mM) of imidazole.Figure 1
Maps of expression vector forsfGFP-TEV-His6 Nd1–6 used in our work. The vector map was created by Vector NTI software. All variants of sfGFP-TEV-His6 use the same vector pT7His derived from pET21a. The coding region in black will produce sfGFP-TEV-His6 Nd1–6.


### 3.2. Fusion of sfGFP to TEV Greatly Increases the Soluble Production of TEV Protease

After autoinduction,sfGFP-TEV-His6 Nd1–6 were all purified by Ni-NTA affinity chromatography and Q anion exchange chromatography. After purification, there was an obvious main band around the molecular weight of 53 kDa (Figures 2(a) and 2(b)). Table 3 summarizes the purification results from 1-L culture medium. According to Bandscan software analysis, all variants of fusion protease were obtained with over 96% purity. Among them, Nd2, Nd4, and Nd5 were purified with over 98% purity. With the fusion of sfGFP, all variants could be purified by two-step chromatography with soluble production of over 200 mg. In particular, we could obtain around 320 mg of sfGFP-TEV Nd2 from 1-L culture medium. Because the molecular weight of sfGFP-TEV Nd2 and TEV-His6 was 53.8 kDa and 28.8 kDa, respectively, 320 mg/L of sfGFP-TEV Nd2’s effective TEV composition was close to 171 mg/L (320*28.8/53.8=171) of TEV-His6. We also constructed the control expression vector for TEV protease without any tags, but there was almost no detectable TEV protease expressed under the same induction condition (data not shown). Therefore, the fusion of sfGFP to TEV significantly increases the soluble production of TEV protease.Table 3
Purification results ofsfGFP-TEV-His6 Nd1–6 and TEV-His6 collected from 1-L of expression culture medium.
AbbreviationTheoretical MW (kDa)Total protein after Ni-NTA (mg)PurityTotal protein after High Q (mg)PurityTotal activity (μmol/h)Specific activity (μmol/h/mg)Nd153.5244±190%221±296%7.3±0.20.067±0.002Nd253.8334±294%323±198%13.7±0.30.085±0.002Nd354.1220±493%211±397%8.0±0.10.076±0.001Nd454.5304±693%293±498%11.9±0.50.082±0.003Nd555.0299±193%288±298%11.3±0.60.079±0.005Nd654.2308±593%300±496%12.0±0.50.080±0.003TEV28.8140±398%——12.6±0.60.090±0.005SDS-PAGE analysis of the expression and purification ofsfGFP-TEV-His6 Nd1–6 and TEV-His6. (a) Lanes 1–3 are the result for TEV-His6: Lane 1: autoinduced whole-bacterial lysate; Lane 2: flow through from Ni-NTA affinity chromatography; Lane 3: the purified TEV-His6 eluted from Ni-NTA affinity chromatography. Lanes 4–7 are the result for sfGFP-TEV-His6 Nd2: Lane 4: autoinduced whole-bacterial lysate; Lane 5: flow through from Ni-NTA affinity chromatography; Lane 6: the purified sfGFP-TEV-His6 Nd2 eluted from Ni-NTA affinity chromatography; Lane 7: the pooled sample of purified sfGFP-TEV-His6 Nd2 eluted from Q anion exchange chromatography. (b) The results for sfGFP-TEV-His6 Nd1 and Nd3–6. “Ni” represents the results of the purified protein eluted from Ni-NTA affinity chromatography; “Q” represents the pooled sample of purified protein eluted from Q anion exchange chromatography. “M” in (a) and (b) represents the protein marker.
(a)(b)


### 3.3. Purification of TEV-His6 and MBP-EGFP

We have also expressed and purified TEV-His6 as control and MBP-EGFP as TEV protease’s substrate. During expression, the MBP tag of MBP-LTL-TEV-His6 would be cleaved and then TEV-His6 was released. By Ni-NTA affinity chromatography and further dialysis, about 140 mg of TEV-His6 could be obtained from 1-L culture medium with around 98% purity. The electrophoresis results show that molecular weight of TEV-His6 is around 29 kDa (Figure 2(a)). The substrate MBP-EGFP could also be purified with over 95% purity by Amylose affinity chromatography.


### 3.4. Cleavage Activity Assay of sfGFP-TEV and TEV

The cleavage activity assay ofsfGFP-TEV-His6 Nd1–6 and TEV-His6 could be determined by cleaving the substrate MBP-EGFP at the cleavage site “ENLYFQ/G” between MBP and EGFP. By SDS-PAGE, the remaining MBP-EGFP could be separated sufficiently with released MBP and EGFP (Figure 3(a)). After we set the quantity of MBP-EGFP at 0 min as 100%, the time course curve could be plotted by quantitatively analyzing the digested MBP-EGFP at the given time. Figure 3(b) shows the time course curve of sfGFP-TEV-His6 Nd1–6, TEV-His6, and 2% TEV-His6. Compared with the time course curve of TEV-His6, we found that sfGFP-TEV-His6 Nd1–6 had different degrees of loss of catalytic activity. Among them, Nd2 had the closest curve to TEV-His6. Ranking the cleavage rate at 60 minutes, the second highest ranked Nd2 could digest around 66% substrate, which retained about 95% catalytic activity of TEV-His6. Moreover, TEV-His6 and all variants of sfGFP-TEV-His6 except Nd1 could efficiently cleave over 98% substrate after incubation for 4 hours at 17°C. However, the control 2% TEV-His6 could only cleave less than 7% substrate under the same condition (Figure 3(c)). In conclusion, sfGFP-TEV-His6 Nd2 retained the most of catalytic activity among all variants.SDS-PAGE and time course analysis of catalytic activity on MBP-EGFP ofsfGFP-TEV-His6 and TEV-His6. (a) One representative of SDS-PAGE analysis. 100 μg MBP-EGFP was incubated with 2 μg sfGFP-TEV-His6 Nd2. Lane 1 is the protein marker. Lanes 2–11 represent different incubation time (0, 5, 10, 20, 40, 60, 90, 120, 180, 240 minutes, resp.). (b) The time course curve analysis. The time course curve was plotted according to the quantitative analysis of the SDS-PAGE by Bandscan 4.30. Each data point was the average of three independent tests. (c) Bar representation based on time course curve. The black bars represent the percentage of the digested MBP-EGFP after incubation for 60 minutes. The striped bars represent the percentage after incubation for 240 minutes. The ranking of the bar is based on the sorting result of cleavage efficiency after incubation for 60 minutes. The height of the bar is the average of three independent tests with standard error on top of the bar.
(a)(b)(c)Fusion tags are widely used to facilitate protein expression and purification. However, due to its drawback in structural and functional studies, these tags always need to be removed by various proteases. TEV protease is an ideal protease receiving most attention, thanks to its high specificity as well as toleration of a wide range of temperatures and presence of detergents [23]. One bottleneck for TEV protease is low soluble production due to its poor solubility. Researchers have tried many strategies including in silico design [24], direct-evolution [15], or coexpression with chaperone to increase its soluble production. These efforts have raised the production from 1 mg/L to 65 mg/L culture [16]. More recently, Blommel and Fox reported a production of 400 mg/L culture by optimizing each step in expression, and purification [17]. However, the whole process of expression, purification and characterization of recombinant TEV protease was not visible to naked eye. Our attempts to express recombinant TEV protease fused with commonly used GST, TRX, and NusA tags all failed (data not shown). GST and TRX fused TEV proteases were most in the inclusion body and NusA fusion strategy gave less than 50% full length fusion protein.In this paper, we introduce a novel method to increase the soluble production of TEV protease by fusingsfGFP to TEV protease. The results show that the production of sfGFP-TEV-His6 Nd2 fusion protease reached 320 mg/L culture. Thanks to sfGFP’s high folding kinetics, thermodynamic stability, sfGFP might work as a platform for the folding of TEV protease to prevent the formation of inclusion body. Compared with MBP which brings high metabolic burden for the host, sfGFP is much smaller and has fluorescence easy for detection. Figure 4 showed that the expression (Figure 4(a)) and purification (Figure 4(b)) procedure of sfGFP-tagged fusion protein can be monitored and quantified real-time by the fluorescence emitted fromsfGFP, thus greatly simplified the procedure of sfGFP tagged target proteins expression and purification. We suggest that sfGFP could be employed as a colored solubility-enhancing tag for other small proteins with poor soluble production.The quantification ofsfGFP fluorescence during sfGFP-TEV-His6 Nd2 expression and purification. (a) The fluorescence intensity versus time curve of sfGFP-TEV-His6 Nd2 by autoinduction at 19°C. The fluorescence of 100 μL cultured E. coli cells in the autoinduction medium was collected using bottom reading method with 485 nm excitation filter and 535 nm emission filter. The height of the bar is the average of three independent tests with standard error on top of the bar. (b) One representative sfGFP fluorescence quantification during Ni-NTA purification. The fluorescence was collected using top reading method with 485 nm excitation filter and 535 nm emission filter. (−): uninduced cell lysate; (+): induced whole cell lysate; S↑: supernatant of the sonication; F.T.: flow through fraction from Ni-NTA chromatography; Wash: wash fraction from Ni-NTA chromatography; E.: eluated fraction by imidazole from Ni-NTA chromatography.
(a)(b)Catalytic activity is another important factor to be examined in our work. We constructed 6 variants ofsfGFP-TEV-His6 in all. The catalytic tests show that sfGFP-TEV-His6 Nd2 with a linker of only five residues “GSKGP” has the closest catalytic activity to TEV-His6. After one-hour incubation at 17°C, over 65% MBP-EGFP could be cleaved by sfGFP-TEV-His6 Nd2 while TEV-His6 cleaved around 70% substrate (Figure 3). In contrast, sfGFP-TEV-His6 Nd1 has the lowest specific activity, which might be explained by the importance of three residues “KGP” on the correct folding and stability of TEV protease.When preserved in4°C for a long time, TEV-His6 was not stable and would precipitate and completely lose the catalytic activity within one week (data not shown). However, sfGFP-TEV-His6 Nd2 would not precipitate for more than one month and still retained about 60% catalytic activity, which showed much higher stability than original TEV-His6. The sfGFP tag not only increased the solubility of the target protein during expression and purification but also increased its stability. Though the increase of effective TEV protease yield of sfGFP-TEV was only ~22% (from 140 mg to 171 mg TEV-His6 per liter culture), during the long time cleavage experiment, the increased stability of sfGFP-TEV significantly outrun the original TEV protease widely used. This feature is vital because structural genomics required large-scale production of tag-free target proteins by TEV protease. Besides, the fluorescence characteristic of sfGFP tag provided an accurate, visible, and high-throughput measurement to quantify the fused target protein. The trace existence of sfGFP tagged TEV can be sensitive and easily detected by spectrofluorometer. By detecting the sfGFP fluorescence intensity, we can also accurately quantify the recombinant sfGFP-TEV protease. Like the original TEV protease, the His6 tag of sfGFP-TEV makes it very easy to remove sfGFP-TEV from cleaved target protein by Ni-NTA chromatography after cleavage experiment.In nature, evolution has shown its power of merging different domains to create a novel enzyme with great property. With rational design, we can also take advantage of available proteins to improve the property of certain enzymes. Our research showed thatsfGFP tag significantly improved the solubility, expression level, and stability of TEV protease, which is important for the large-scale production of functional TEV protease used in structural genomics research.


## 3.1. Construction of Expression Vector for sfGFP-TEV and TEV

In order to maximize the expression level of the recombinantsfGFP-TEV proteases, we first synthesized the sfGFP gene according to the synonymous codon choice which is optimal for the Escherichia coli translational system. Figure 1 shows the vector map we used for high-level expression of sfGFP-TEV-His6 Nd1–6. The sfGFP-TEV coding sequence was cloned to the pET derived vector pT7His which possesses the strong bacteriophage T7 promoter, ensuring the high level expression of target protein. Considering that the linker between sfGFP and TEV might have effects on the stability and catalytic activity of fusion protease, we constructed 6 variants of sfGFP-TEV-His6 with different linkers. The linker here is defined as the peptide between C-terminus of sfGFP “THG” and N-terminus of TEV “RDYNP.” The composition of different linkers with lengths varying from 2 to 14aa could be referred to Table 2. We also incorporated a small peptide “GGG” at the C-terminus of TEV; so the C-terminuses of sfGFP-TEV-His6 Nd1–6 and TEV-His6 are all “LMNEGGGLEHHHHHH.” Our first attempt of sfGFP-TEV vector construction did not include the GGG small peptide between TEV and C-terminus His6 tag. However, during the Ni-NTA purification step, more than 70% expressed fusion protein did not bind with the Ni-NTA resin (data not shown). Perhaps the steric structure of TEV hindered His6 tag from binding with Ni-NTA resin. So we added the flexible GGG peptide between TEV and His6 tag. Almost all of the new version fusion protein can bind with Ni-NTA in the buffer containing relatively high concentration (20 mM) of imidazole.Figure 1
Maps of expression vector forsfGFP-TEV-His6 Nd1–6 used in our work. The vector map was created by Vector NTI software. All variants of sfGFP-TEV-His6 use the same vector pT7His derived from pET21a. The coding region in black will produce sfGFP-TEV-His6 Nd1–6.


## 3.2. Fusion of sfGFP to TEV Greatly Increases the Soluble Production of TEV Protease

After autoinduction,sfGFP-TEV-His6 Nd1–6 were all purified by Ni-NTA affinity chromatography and Q anion exchange chromatography. After purification, there was an obvious main band around the molecular weight of 53 kDa (Figures 2(a) and 2(b)). Table 3 summarizes the purification results from 1-L culture medium. According to Bandscan software analysis, all variants of fusion protease were obtained with over 96% purity. Among them, Nd2, Nd4, and Nd5 were purified with over 98% purity. With the fusion of sfGFP, all variants could be purified by two-step chromatography with soluble production of over 200 mg. In particular, we could obtain around 320 mg of sfGFP-TEV Nd2 from 1-L culture medium. Because the molecular weight of sfGFP-TEV Nd2 and TEV-His6 was 53.8 kDa and 28.8 kDa, respectively, 320 mg/L of sfGFP-TEV Nd2’s effective TEV composition was close to 171 mg/L (320*28.8/53.8=171) of TEV-His6. We also constructed the control expression vector for TEV protease without any tags, but there was almost no detectable TEV protease expressed under the same induction condition (data not shown). Therefore, the fusion of sfGFP to TEV significantly increases the soluble production of TEV protease.Table 3
Purification results ofsfGFP-TEV-His6 Nd1–6 and TEV-His6 collected from 1-L of expression culture medium.
AbbreviationTheoretical MW (kDa)Total protein after Ni-NTA (mg)PurityTotal protein after High Q (mg)PurityTotal activity (μmol/h)Specific activity (μmol/h/mg)Nd153.5244±190%221±296%7.3±0.20.067±0.002Nd253.8334±294%323±198%13.7±0.30.085±0.002Nd354.1220±493%211±397%8.0±0.10.076±0.001Nd454.5304±693%293±498%11.9±0.50.082±0.003Nd555.0299±193%288±298%11.3±0.60.079±0.005Nd654.2308±593%300±496%12.0±0.50.080±0.003TEV28.8140±398%——12.6±0.60.090±0.005SDS-PAGE analysis of the expression and purification ofsfGFP-TEV-His6 Nd1–6 and TEV-His6. (a) Lanes 1–3 are the result for TEV-His6: Lane 1: autoinduced whole-bacterial lysate; Lane 2: flow through from Ni-NTA affinity chromatography; Lane 3: the purified TEV-His6 eluted from Ni-NTA affinity chromatography. Lanes 4–7 are the result for sfGFP-TEV-His6 Nd2: Lane 4: autoinduced whole-bacterial lysate; Lane 5: flow through from Ni-NTA affinity chromatography; Lane 6: the purified sfGFP-TEV-His6 Nd2 eluted from Ni-NTA affinity chromatography; Lane 7: the pooled sample of purified sfGFP-TEV-His6 Nd2 eluted from Q anion exchange chromatography. (b) The results for sfGFP-TEV-His6 Nd1 and Nd3–6. “Ni” represents the results of the purified protein eluted from Ni-NTA affinity chromatography; “Q” represents the pooled sample of purified protein eluted from Q anion exchange chromatography. “M” in (a) and (b) represents the protein marker.
(a)(b)


## 3.3. Purification of TEV-His6 and MBP-EGFP

We have also expressed and purified TEV-His6 as control and MBP-EGFP as TEV protease’s substrate. During expression, the MBP tag of MBP-LTL-TEV-His6 would be cleaved and then TEV-His6 was released. By Ni-NTA affinity chromatography and further dialysis, about 140 mg of TEV-His6 could be obtained from 1-L culture medium with around 98% purity. The electrophoresis results show that molecular weight of TEV-His6 is around 29 kDa (Figure 2(a)). The substrate MBP-EGFP could also be purified with over 95% purity by Amylose affinity chromatography.


## 3.4. Cleavage Activity Assay of sfGFP-TEV and TEV

The cleavage activity assay ofsfGFP-TEV-His6 Nd1–6 and TEV-His6 could be determined by cleaving the substrate MBP-EGFP at the cleavage site “ENLYFQ/G” between MBP and EGFP. By SDS-PAGE, the remaining MBP-EGFP could be separated sufficiently with released MBP and EGFP (Figure 3(a)). After we set the quantity of MBP-EGFP at 0 min as 100%, the time course curve could be plotted by quantitatively analyzing the digested MBP-EGFP at the given time. Figure 3(b) shows the time course curve of sfGFP-TEV-His6 Nd1–6, TEV-His6, and 2% TEV-His6. Compared with the time course curve of TEV-His6, we found that sfGFP-TEV-His6 Nd1–6 had different degrees of loss of catalytic activity. Among them, Nd2 had the closest curve to TEV-His6. Ranking the cleavage rate at 60 minutes, the second highest ranked Nd2 could digest around 66% substrate, which retained about 95% catalytic activity of TEV-His6. Moreover, TEV-His6 and all variants of sfGFP-TEV-His6 except Nd1 could efficiently cleave over 98% substrate after incubation for 4 hours at 17°C. However, the control 2% TEV-His6 could only cleave less than 7% substrate under the same condition (Figure 3(c)). In conclusion, sfGFP-TEV-His6 Nd2 retained the most of catalytic activity among all variants.SDS-PAGE and time course analysis of catalytic activity on MBP-EGFP ofsfGFP-TEV-His6 and TEV-His6. (a) One representative of SDS-PAGE analysis. 100 μg MBP-EGFP was incubated with 2 μg sfGFP-TEV-His6 Nd2. Lane 1 is the protein marker. Lanes 2–11 represent different incubation time (0, 5, 10, 20, 40, 60, 90, 120, 180, 240 minutes, resp.). (b) The time course curve analysis. The time course curve was plotted according to the quantitative analysis of the SDS-PAGE by Bandscan 4.30. Each data point was the average of three independent tests. (c) Bar representation based on time course curve. The black bars represent the percentage of the digested MBP-EGFP after incubation for 60 minutes. The striped bars represent the percentage after incubation for 240 minutes. The ranking of the bar is based on the sorting result of cleavage efficiency after incubation for 60 minutes. The height of the bar is the average of three independent tests with standard error on top of the bar.
(a)(b)(c)Fusion tags are widely used to facilitate protein expression and purification. However, due to its drawback in structural and functional studies, these tags always need to be removed by various proteases. TEV protease is an ideal protease receiving most attention, thanks to its high specificity as well as toleration of a wide range of temperatures and presence of detergents [23]. One bottleneck for TEV protease is low soluble production due to its poor solubility. Researchers have tried many strategies including in silico design [24], direct-evolution [15], or coexpression with chaperone to increase its soluble production. These efforts have raised the production from 1 mg/L to 65 mg/L culture [16]. More recently, Blommel and Fox reported a production of 400 mg/L culture by optimizing each step in expression, and purification [17]. However, the whole process of expression, purification and characterization of recombinant TEV protease was not visible to naked eye. Our attempts to express recombinant TEV protease fused with commonly used GST, TRX, and NusA tags all failed (data not shown). GST and TRX fused TEV proteases were most in the inclusion body and NusA fusion strategy gave less than 50% full length fusion protein.In this paper, we introduce a novel method to increase the soluble production of TEV protease by fusingsfGFP to TEV protease. The results show that the production of sfGFP-TEV-His6 Nd2 fusion protease reached 320 mg/L culture. Thanks to sfGFP’s high folding kinetics, thermodynamic stability, sfGFP might work as a platform for the folding of TEV protease to prevent the formation of inclusion body. Compared with MBP which brings high metabolic burden for the host, sfGFP is much smaller and has fluorescence easy for detection. Figure 4 showed that the expression (Figure 4(a)) and purification (Figure 4(b)) procedure of sfGFP-tagged fusion protein can be monitored and quantified real-time by the fluorescence emitted fromsfGFP, thus greatly simplified the procedure of sfGFP tagged target proteins expression and purification. We suggest that sfGFP could be employed as a colored solubility-enhancing tag for other small proteins with poor soluble production.The quantification ofsfGFP fluorescence during sfGFP-TEV-His6 Nd2 expression and purification. (a) The fluorescence intensity versus time curve of sfGFP-TEV-His6 Nd2 by autoinduction at 19°C. The fluorescence of 100 μL cultured E. coli cells in the autoinduction medium was collected using bottom reading method with 485 nm excitation filter and 535 nm emission filter. The height of the bar is the average of three independent tests with standard error on top of the bar. (b) One representative sfGFP fluorescence quantification during Ni-NTA purification. The fluorescence was collected using top reading method with 485 nm excitation filter and 535 nm emission filter. (−): uninduced cell lysate; (+): induced whole cell lysate; S↑: supernatant of the sonication; F.T.: flow through fraction from Ni-NTA chromatography; Wash: wash fraction from Ni-NTA chromatography; E.: eluated fraction by imidazole from Ni-NTA chromatography.
(a)(b)Catalytic activity is another important factor to be examined in our work. We constructed 6 variants ofsfGFP-TEV-His6 in all. The catalytic tests show that sfGFP-TEV-His6 Nd2 with a linker of only five residues “GSKGP” has the closest catalytic activity to TEV-His6. After one-hour incubation at 17°C, over 65% MBP-EGFP could be cleaved by sfGFP-TEV-His6 Nd2 while TEV-His6 cleaved around 70% substrate (Figure 3). In contrast, sfGFP-TEV-His6 Nd1 has the lowest specific activity, which might be explained by the importance of three residues “KGP” on the correct folding and stability of TEV protease.When preserved in4°C for a long time, TEV-His6 was not stable and would precipitate and completely lose the catalytic activity within one week (data not shown). However, sfGFP-TEV-His6 Nd2 would not precipitate for more than one month and still retained about 60% catalytic activity, which showed much higher stability than original TEV-His6. The sfGFP tag not only increased the solubility of the target protein during expression and purification but also increased its stability. Though the increase of effective TEV protease yield of sfGFP-TEV was only ~22% (from 140 mg to 171 mg TEV-His6 per liter culture), during the long time cleavage experiment, the increased stability of sfGFP-TEV significantly outrun the original TEV protease widely used. This feature is vital because structural genomics required large-scale production of tag-free target proteins by TEV protease. Besides, the fluorescence characteristic of sfGFP tag provided an accurate, visible, and high-throughput measurement to quantify the fused target protein. The trace existence of sfGFP tagged TEV can be sensitive and easily detected by spectrofluorometer. By detecting the sfGFP fluorescence intensity, we can also accurately quantify the recombinant sfGFP-TEV protease. Like the original TEV protease, the His6 tag of sfGFP-TEV makes it very easy to remove sfGFP-TEV from cleaved target protein by Ni-NTA chromatography after cleavage experiment.In nature, evolution has shown its power of merging different domains to create a novel enzyme with great property. With rational design, we can also take advantage of available proteins to improve the property of certain enzymes. Our research showed thatsfGFP tag significantly improved the solubility, expression level, and stability of TEV protease, which is important for the large-scale production of functional TEV protease used in structural genomics research.



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*Source: 591923-2010-02-23.xml*