# The Biological Side of Water-Soluble Arene Ruthenium Assemblies

Authors: Bruno Therrien; Julien Furrer
Journal: Advances in Chemistry
 (2014)

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## Abstract

This review article covers the synthetic strategies, structural aspects, and host-guest properties of ruthenium metalla-assemblies, with a special focus on their use as drug delivery vectors. The two-dimensional metalla-rectangles show interesting host-guest possibilities but seem less appropriate for being used as drug carriers. On the other hand, metalla-prisms allow encapsulation and possible targeted release of bioactive molecules and consequently show some potential as drug delivery vectors. The reactivity of these metalla-prisms can be fine-tuned to allow a fine control of the guest’s release. The larger metalla-cubes can be used to stabilize the formation of G-quadruplex DNA and can be used to encapsulate and release photoactive molecules such as porphins. These metalla-assemblies demonstrate great prospective in photodynamic therapy.


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## Body

## 1. Introduction

Anticancer platinum complexes dominate the field of metals in cancer chemotherapy, mainly because of the considerable research effort that followed the fortuitous discovery of the anticancer property of cisplatin (Figure1) in the late 1960s [1]. Unfortunately, the use of cisplatin is restricted because of severe dose-limiting side effects, which arise from the indiscriminate uptake of the drug into all rapidly dividing cells. Indeed the platinum atom is known to efficiently bind to serum proteins, especially human serum albumin (HsA) [2]. Once inside cells, aquated cisplatin species will mainly bind at the N7 position of guanine, leading to significant distortions of the DNA helix [3]. These DNA lesions are thought to prevent replication and transcription and ultimately to lead to cellular apoptosis [3].Figure 1
The three platinum-based anticancer drugs, cisplatin, carboplatin, and oxaliplatin, which have gained worldwide marketing approval for humans [4].The severe side effects of cisplatin have led to a massive investigation into the cytotoxicity and genotoxicity of platinum-based compounds, in order to elucidate their molecular mechanisms and identify key biomolecular targets for rational drug design [6–8]. In the 30 years since cisplatin’s first approval for human use, only two compounds have been approved worldwide (oxaliplatin and carboplatin, Figure 1) and another three gaining marketing approval in individual nations (Nedaplatin, Lobaplatin, and Heptaplatin) [4]. Currently, there are four mononuclear platinum drugs in various stages of clinical trials, with two of these being close to clinical recognition (satraplatin [9] and picoplatin [10]). Two other drugs (ProLindac and Lipoplatin) [11–14] will become the first polymer/liposomal-based platinum drugs on the market if approved [4].Synthesized and developed contemporarily to mononuclear platinum complexes, polynuclear platinum compounds have received a great deal of attention [15]. One of these compounds, triplatin tetranitrate (BBR3464, Figure 2) [5, 16, 17], has entered four consecutive phase II trials, three of them unsuccessful, and the drug has never moved into phase III [4]. The results of the remaining phase II clinical trial on metastatic pancreatic cancer which started in 2001 have yet to be published. However, it was reported that BBR3464 causes so severe large dose-limiting side effects where the maximum tolerated dose (MTD) in humans was considerably lower than the MTD for all the platinum-based drugs currently used in the clinic [18].Figure 2
Structure of the first polynuclear platinum compound, triplatin tetranitrate (BBR3464), that has entered clinical trials [5].A challenge of anticancer treatment is the specific delivery of the drugs in order to avoid deleterious effects on normal cells. In fact, most of the inorganic anticancer drugs have potent effects also on normal cells due to the strong similarity of the mechanisms of growth regulation of normal cells as compared to their transformed counterparts.Recent developments in nanotechnology have aimed at delivering anticancer drugs in tumor tissue via encapsulation in the so-called drug delivery systems [19, 20]. The delivery strategy of anticancer drugs is based upon the particular structure of tumor neoangiogenic vessels that allow the passive targeting or enhanced permeability and retention effect (EPR effect) [21]. The addition of targeting moieties on the surface of such drug delivery systems, such as specific antibodies recognition moieties, can further enhance the active targeting to tumor cells. As such, a lot of effort has been put into the development of such systems that would alleviate the inherent toxicity of platinum drugs and resistance issues. Liposomes [22], polymers [23–25], dendrimers [26], organic macrocycles [27], nanoparticles [28], viruses [29], and carbon nanotubes [30, 31] have all shown promises as drug delivery vehicles for platinum therapeutics.Metalla-assemblies have started to attract considerable attention, but these systems have remained relativity unexplored as drug delivery vectors [32]. The assembly of transition-metal complexes with polydentate ligands to give discrete supramolecular structures has been studied by several groups. Pioneered by Fujita et al. [33] and developed by several groups in the 1990s [34, 35], the combination of 90° coordination building blocks and linear ligands to form square (four metal centers bridged by four equivalent bidentate ligands, Figure 3) and rectangular assemblies (four metal centers bridged by ligands of different lengths) has been widely explored.Figure 3
First metalla-square obtained by combining 90° coordination building blocks and linear ligands, adapted from [33].A few years later, the same approach was used to generate discrete three-dimensional assemblies [36–38]. So far, a multitude of two- and three-dimensional structures incorporating transition metals with square-planar geometry have been synthesized [34, 39]. These molecular boxes have been used to generate confined environments to encapsulate guest molecules, to protect sensitive or unstable molecules, to recognize and trap specific guest molecules, or to act as microreactors for specific reactions [32].Recently, dipalladium(II) molecular cages of the formula [Pd2L4](X)4 (L=2,6-bis(pyridin-3-ylethynyl)pyridine; X=BF4- or SbF6-) have been shown to encapsulate two cisplatin molecules within their cavities (Figure 4) [40]. These cages represent the first example of a discrete metalla-assembly encapsulating a FDA-approved inorganic drug molecule. Additionally, the [(cisplatin)2⊂Pd2L4]4+ host-guest adduct can be quantitatively disassembled upon the addition of a competing ligand, releasing cisplatin in an acetonitrile solution. This property, if extended to biological media, is particularly appealing in the context of drug delivery, as it would potentially enable the controlled release of an encapsulated drug from the metalla-assembly. Unfortunately, the drawback of these palladium assemblies is their lability in water and accordingly their instability in biological media.Figure 4
Molecular structure of [(cisplatin)2⊂Pd2L4]4+, adapted from [40].The cytotoxic effects of ruthenium compounds are known since the mid-50s [41], but these compounds have mostly remained disregarded, partly because of their poor water solubility, and mostly because the discovery of the anticancer properties of cisplatin in the mid-60s has inclined the research towards platinum compounds. The potential of ruthenium compounds as anticancer drugs has been revived and really exploited only in the mid-80s, with the pioneer work of Clarke on the activation by reduction assumption (Figure 5) [42]. This assumption is based on the generally accepted statement that ruthenium(II) complexes are more reactive than ruthenium(III) complexes. Ruthenium complexes are therefore administered as much less active, nontoxic ruthenium(III) compounds, which are reduced in toxic ruthenium(II) complexes in cancer cells. Only in cancer cells is a chemically reducing environment created, as a result of increased metabolic rate. Scientists working in this field often make a point of comparing ruthenium compounds favorably to platinum compounds in terms of its analogous ligand exchange abilities, lack of cross-resistance, and lower toxicity [43–45].Figure 5
Schematic representation of the postulated activation by reduction mechanism, adapted from [42].Currently, two inorganic monoruthenium(III) complexes NAMI-A and KP1019 have shown remarkable activities (Figure6) [46, 47]. The more water-soluble version of KP1019, the sodium salt termed KP1339 (Figure 6), has recently finished a successful clinical phase I trial, where excellent activity, especially against neuroendocrine tumors, was observed [48].Figure 6
Molecular structure of the inorganic ruthenium(III) complexes: NAMI-A, KP1019, and KP1339.Since the mid-1990s, half-sandwich ruthenium(II) compounds have started to attract considerable attention [49]; in particular, the RAPTA family was developed in the group of Dyson [50–52], and the RM175 and ONCO4417 compounds were developed in the group of Sadler (Figure 7) [53]. These derivatives exhibit very promising characteristicsin vitro (RM175) andin vivo(RAPTA). The RAPTA complexes can be considered noncytotoxicin vitrobut show antimetastatic activity and have indicated selectivity to cancer cells over nontumor cells [51]. The RAPTA complexes are inactive against primary tumors, but they appear to reduce the number and weight of metastasis cells. In the case of RM175, it shows high levels of toxicity towards cancer cells, affecting DNA via a parallel interaction, thus resulting in apoptosis [53]. Similarly ONCO4417 was shown to induce apoptosis as well as to halt cell reproduction in various cancer types [53].Figure 7
Half-sandwich ruthenium(II) complexes: RAPTA, RM175, and ONCO4417.As such, arene ruthenium metalla-assemblies have started to attract attention as a potential new class of drug delivery vector. In the following, we will cover some synthetic and structural aspects of arene ruthenium metalla-assemblies, as well as their drug delivery potential. To be efficient, metalla-assemblies must exhibit certain stability in order to achieve controlled drug release in the body. In addition, the presence of arene ruthenium building blocks can only be beneficial if selectivity for cancer cells is obtained. A special emphasis has been placed on our contributions to this field over the last years, but important inputs from other groups will be also discussed.


## 2. Water-Soluble Arene Ruthenium Complexes

The water solubility of arene ruthenium complexes is known for 40 years, thanks to Zelonka and Baird who studied the behavior of the benzene ruthenium dichloride dimer in water [54]. Indeed, upon dissolution of [(benzene)RuCl2]2 in water, they observed by 1H NMR spectroscopy the formation of aqua species. The complete assignment of the signals was established later [55] but confirmed the observation made by Zelonka and Baird that arene ruthenium dichloride dimers form aqua species in water without rupture of the arene ruthenium bond (Figure 8). With the stability of the arene ruthenium bond in water being established, the chemistry of arene ruthenium complexes in aqueous solution is now flourishing [55–57].Figure 8
Aquation of arene ruthenium dichloride dimers.


### 2.1. Water-Soluble Arene Ruthenium Metalla-Rectangles

The first arene ruthenium metalla-rectangle has been synthesized by Yan et al. in 1997 [58]. It was shown that the dinuclear cation [{(p-cymene)Ru(MeOH)}2(ox)]2+, p-cymene = p-iPrC6H4Me, incorporating an oxalato (ox) bridging ligand reacts with 4,4′-bipyridine (bipy) to form in quantitative yield the tetranuclear tetracationic metalla-rectangle [Ru4(p-cymene)4(ox)2(bipy)2]4+ (Figure 9). For ten years, this simple strategy to form metalla-assemblies did not find much attention; however, it is now one of the methods of choice to form arene ruthenium metalla-assemblies, and it remains a milestone in the field.Figure 9
Molecular structure of the first arene ruthenium metalla-rectangle, [Ru4(p-cymene)4(ox)2(bipy)2]4+, adapted from [58].In 2009, two groups independently prepared water-soluble arene ruthenium metalla-rectangles in view of evaluating them as anticancer agents [59, 60]. Navarro and Barea have combined hoxonato dinuclear p-cymene ruthenium clips with bipy and phen (phen = 4,7-phenanthroline), while Therrien and his coworkers have used various arenes (p-cymene and hexamethylbenzene), two different bridging ligands (2,5-dihydroxy-1,4-benzoquinonato = dhbq; 2,5-dichloro-1,4-benzoquinonato = dcbq), with bipy and bpe (bpe = 1,2-bis(4-pyridyl)ethylene), to obtain analogous structures. In both series, the tetracationic metalla-rectangles were found to be cytotoxicin vitro, showing activity in the μM range against ovarian cancer cell lines. In addition, strong interaction with DNA was demonstrated for the hoxonato derivatives, thus confirming the biological relevance of arene ruthenium metalla-assemblies [59].Following these first studies, Chi, Stang and coworkers have reported several analogous metalla-rectangles, each being formed by combining dinuclear arene ruthenium clips and bipyridyl connectors [39, 61–65]. The IC50 values for particular combinations of clips and connectors are in the lower micromolar range, while others show poor activities. However, at this stage, the parameters dictating the biological activity of these metalla-rectangles remain unclear. It has been suggested that the size and the nature of the connectors are important factors, as well as the charge and lipophilicity of the assembly [66]; however, replacing the arene ruthenium units by other half-sandwich complexes showed almost no impact on the activity [67, 68]. Clearly, these studies suggest that the best combination of arenes, metals, and connectors has yet to be found.


### 2.2. Water-Soluble Arene Ruthenium Metalla-Prisms and Metalla-Cubes

In parallel to the development of metalla-rectangles, three-dimensional metalla-assemblies have been prepared following the same strategy [32], simply by replacing the bidentate connectors by tridentate or tetradentate ligands. For example, combining oxalato bridged dinuclear clips with tridentate 2,4,6-tris(pyridine-4-yl)-1,3,5-triazine (tpt) panels affords in excellent yield the metalla-prism [Ru6(p-cymene)6(ox)3(tpt)2]6+ (Figure 10), which was isolated as its triflate salt [69]. Similarly, the octanuclear octacationic metalla-cube, [Ru8(p-cymene)8(ox)4(tpp)2]8+, was obtained by combining oxalato bridged dinuclear clips with 5,10,15,20-tetra(pyridin-4-yl)porphyrin (tpp) tetradentate panels [70]. Throughout the paper, metalla-prisms are defined as six metal centers connected by two tridentate panels and three bridging ligands, while metalla-cubes are defined as eight metal centers connected by two tetradentate panels and four bridging ligands.Figure 10
Molecular structure of the arene ruthenium metalla-prism, [Ru6(p-cymene)6(ox)3(tpt)2]6+, adapted from [58].Despite possessing a small cavity, with theRu⋯Ru separation within these diruthenium oxalato clips being 5.5 Å, no guest molecules fit in the cavity of the metalla-prism [Ru6(p-cymene)6(ox)3(tpt)2]6+ due to strong π-stacking interactions between the adjacent tpt panels. Therefore, a longer metal⋯metal distance was needed to allow metalla-prisms and metalla-cubes to encapsulate guest molecules. In large three-dimensional assemblies, a cavity capable of accommodating guest molecules does not necessarily insure host-guest chemistry: access to the cavity is critical for the host-guest process to take place. Therefore, unless the assembly possesses the ability to assemble-disassemble at will in solution, a sufficient portal size of the host is an essential feature [32].


### 2.3. Stabilization of the G-Quadruplex DNA Formation Using Metalla-Cubes

In 2009, we have shown that arene ruthenium metalla-cubes [Ru8(η6-arene)8(tpp-M)2(dhbq)4]8+ (arene = toluene, p-cymene, hexamethylbenzene) (Figure 11), connected by two 5,10,15,20-tetra(4-pyridyl)porphyrin (tpp-2H)or 5,10,15,20-tetra(4-pyridyl) porphyrin-Zn(II) (tpp-Zn) tetrapodal subunits, bind strongly to telomeric andc-mycDNA. Their selectivity for quadruplex over duplex DNA is very modest. This is likely a consequence of the octacationic nature of the cubes, which will increase their nonspecific binding to DNA via electrostatic interactions. However, these arene ruthenium metalla-cubes provide an interesting new family of complexes to stabilize quadruplex DNA [71].Figure 11
Molecular structure of the arene ruthenium metalla-cubes, [Ru8(η6-arene)8(tpp-M)2(dhbq)4]8+, adapted from [71].


## 2.1. Water-Soluble Arene Ruthenium Metalla-Rectangles

The first arene ruthenium metalla-rectangle has been synthesized by Yan et al. in 1997 [58]. It was shown that the dinuclear cation [{(p-cymene)Ru(MeOH)}2(ox)]2+, p-cymene = p-iPrC6H4Me, incorporating an oxalato (ox) bridging ligand reacts with 4,4′-bipyridine (bipy) to form in quantitative yield the tetranuclear tetracationic metalla-rectangle [Ru4(p-cymene)4(ox)2(bipy)2]4+ (Figure 9). For ten years, this simple strategy to form metalla-assemblies did not find much attention; however, it is now one of the methods of choice to form arene ruthenium metalla-assemblies, and it remains a milestone in the field.Figure 9
Molecular structure of the first arene ruthenium metalla-rectangle, [Ru4(p-cymene)4(ox)2(bipy)2]4+, adapted from [58].In 2009, two groups independently prepared water-soluble arene ruthenium metalla-rectangles in view of evaluating them as anticancer agents [59, 60]. Navarro and Barea have combined hoxonato dinuclear p-cymene ruthenium clips with bipy and phen (phen = 4,7-phenanthroline), while Therrien and his coworkers have used various arenes (p-cymene and hexamethylbenzene), two different bridging ligands (2,5-dihydroxy-1,4-benzoquinonato = dhbq; 2,5-dichloro-1,4-benzoquinonato = dcbq), with bipy and bpe (bpe = 1,2-bis(4-pyridyl)ethylene), to obtain analogous structures. In both series, the tetracationic metalla-rectangles were found to be cytotoxicin vitro, showing activity in the μM range against ovarian cancer cell lines. In addition, strong interaction with DNA was demonstrated for the hoxonato derivatives, thus confirming the biological relevance of arene ruthenium metalla-assemblies [59].Following these first studies, Chi, Stang and coworkers have reported several analogous metalla-rectangles, each being formed by combining dinuclear arene ruthenium clips and bipyridyl connectors [39, 61–65]. The IC50 values for particular combinations of clips and connectors are in the lower micromolar range, while others show poor activities. However, at this stage, the parameters dictating the biological activity of these metalla-rectangles remain unclear. It has been suggested that the size and the nature of the connectors are important factors, as well as the charge and lipophilicity of the assembly [66]; however, replacing the arene ruthenium units by other half-sandwich complexes showed almost no impact on the activity [67, 68]. Clearly, these studies suggest that the best combination of arenes, metals, and connectors has yet to be found.


## 2.2. Water-Soluble Arene Ruthenium Metalla-Prisms and Metalla-Cubes

In parallel to the development of metalla-rectangles, three-dimensional metalla-assemblies have been prepared following the same strategy [32], simply by replacing the bidentate connectors by tridentate or tetradentate ligands. For example, combining oxalato bridged dinuclear clips with tridentate 2,4,6-tris(pyridine-4-yl)-1,3,5-triazine (tpt) panels affords in excellent yield the metalla-prism [Ru6(p-cymene)6(ox)3(tpt)2]6+ (Figure 10), which was isolated as its triflate salt [69]. Similarly, the octanuclear octacationic metalla-cube, [Ru8(p-cymene)8(ox)4(tpp)2]8+, was obtained by combining oxalato bridged dinuclear clips with 5,10,15,20-tetra(pyridin-4-yl)porphyrin (tpp) tetradentate panels [70]. Throughout the paper, metalla-prisms are defined as six metal centers connected by two tridentate panels and three bridging ligands, while metalla-cubes are defined as eight metal centers connected by two tetradentate panels and four bridging ligands.Figure 10
Molecular structure of the arene ruthenium metalla-prism, [Ru6(p-cymene)6(ox)3(tpt)2]6+, adapted from [58].Despite possessing a small cavity, with theRu⋯Ru separation within these diruthenium oxalato clips being 5.5 Å, no guest molecules fit in the cavity of the metalla-prism [Ru6(p-cymene)6(ox)3(tpt)2]6+ due to strong π-stacking interactions between the adjacent tpt panels. Therefore, a longer metal⋯metal distance was needed to allow metalla-prisms and metalla-cubes to encapsulate guest molecules. In large three-dimensional assemblies, a cavity capable of accommodating guest molecules does not necessarily insure host-guest chemistry: access to the cavity is critical for the host-guest process to take place. Therefore, unless the assembly possesses the ability to assemble-disassemble at will in solution, a sufficient portal size of the host is an essential feature [32].


## 2.3. Stabilization of the G-Quadruplex DNA Formation Using Metalla-Cubes

In 2009, we have shown that arene ruthenium metalla-cubes [Ru8(η6-arene)8(tpp-M)2(dhbq)4]8+ (arene = toluene, p-cymene, hexamethylbenzene) (Figure 11), connected by two 5,10,15,20-tetra(4-pyridyl)porphyrin (tpp-2H)or 5,10,15,20-tetra(4-pyridyl) porphyrin-Zn(II) (tpp-Zn) tetrapodal subunits, bind strongly to telomeric andc-mycDNA. Their selectivity for quadruplex over duplex DNA is very modest. This is likely a consequence of the octacationic nature of the cubes, which will increase their nonspecific binding to DNA via electrostatic interactions. However, these arene ruthenium metalla-cubes provide an interesting new family of complexes to stabilize quadruplex DNA [71].Figure 11
Molecular structure of the arene ruthenium metalla-cubes, [Ru8(η6-arene)8(tpp-M)2(dhbq)4]8+, adapted from [71].


## 3. Encapsulation of Guest Molecules and Host-Guest Properties

### 3.1. Encapsulation of Guest Molecules with Metalla-Rectangles

The ability of the metalla-rectangles [Ru4(p-cymene)4(N∩N)2(dhnq)2]4+ (N∩N = pyrazine, 4,4′-bipyridine, and 1,2-bis(4-pyridyl)ethylene; dhnq = 5,8-dihydroxy-1,4-naphthoquinonato) to host pyrene in solution has been investigated (Figure 12) [72]. In the case of the smallest metalla-rectangle [Ru4(p-cymene)4(pyrazine)2(dhnq)2]4+, no interaction between the metalla-rectangle and pyrene was observed. However, in the case of the medium-sized metalla-rectangle [Ru4(p-cymene)4(4,4′-bipyridine)2(dhnq)2]4+, interactions occurred on the outside of the rectangular assembly, while in the larger metalla-rectangle [Ru4(p-cymene)4(1,2-bis(4-pyridyl)ethylene)2(dhnq)2]4+, the pyrene molecule was found inside the hydrophobic cavity of the metalla-rectangle, thus giving rise to a host-guest system.Molecular structure of the arene ruthenium metalla-rectangles, [Ru4(p-cymene)4(pyrazine)2(dhnq)2]4+ (a), [Ru4(p-cymene)4(4,4′-bipyridine)2(dhnq)2]4+(b), and [Ru4(p-cymene)4(1,2-bis(4-pyridyl))(ethylene)2(dhnq)2]4+(c), adapted from [72].
(a)
(b)
(c)During this study, we exploited two-dimensional1H-DOSY NMR experiments to gain further insight into the nature of the interactions involved between pyrene and the metalla-rectangles. Diffusion-ordered spectroscopy (DOSY) is a well-established NMR technique that allows separating the NMR signals of different species according to their diffusion coefficient [73, 74]. From the DOSY spectra, it is evidenced that the diffusion constants of pyrene and of the medium-sized metalla-rectangle [Ru4(p-cymene)4(4,4′-bipyridine)2(dhnq)2]4+ are unaffected, when mixed together. Therefore, the chemical shifts observed for the host and the guest were attributed to outer interactions. On the other hand, in the case of the larger metalla-rectangle [Ru4(p-cymene)4(1,2-bis(4-pyridyl)ethylene)2(dhnq)2]4+, the interaction with pyrene is totally different. The DOSY spectra show that for a 1 : 1 mixture of pyrene and [Ru4(p-cymene)4(1,2-bis(4-pyridyl) ethylene)2(dhnq)2]4+ both diffusion coefficients are almost equivalent at about 6.3×10-10 m2·s-1 (Figure 13). Consequently, the pyrene appears to be located in the hydrophobic cavity of [Ru4(p-cymene)4(1,2-bis(4-pyridyl)ethylene)2(dhnq)2]4+, which is in accordance with the strong chemical shift observed for both species in the 1H NMR spectra.Figure 13
DOSY1H NMR spectra of pyrene, of the metalla-rectangle [Ru4(p-cymene)4(1,2-bis(4-pyridyl)ethylene)2(dhnq)2]4+, and of additional amounts of pyrene (1 to 3 equivalent) to a 4.0 mM solution of [Ru4(p-cymene)4(1,2-bis(4-pyridyl) ethylene)2(dhnq)2]4+dissolved in CD3CN, adapted from [72].We have subsequently extended this first study to three other guest molecules, anthracene, perylene, and coronene, and used 9,10-dihydroxy-1,4-anthraquinonato (dhaq) and 6,11-dihydroxynaphthacene-5,12-dionato (dhtq) bridging ligands in addition to 5,8-dihydroxy-1,4-naphthoquinonato (dhnq) [75]. As observed with the metalla-rectangles [Ru4(p-cymene)4(pyrazine)2(dhnq)2]4+, [Ru4(p-cymene)4(4,4′-bipyridine)2(dhnq)2]4+, and [Ru4(p-cymene)4(1,2-bis(4-pyridyl) ethylene)2(dhnq)2]4+, the largest metalla-rectangles incorporating 1,2-bis(4-pyridyl) ethylene linkers are able to host an anthracene, pyrene, perylene, or coronene molecule in their cavity, while the medium-sized metalla-rectangles incorporating 4,4′-bipyridine linkers are only able to encapsulate anthracene. However, out-of-cavity interactions are observed between these 4,4′-bipyridine-containing metalla-rectangles and pyrene, perylene, or coronene. In contrast, the small pyrazine-containing metalla-rectangles showed no interaction in solution with this series of planar aromatic molecules. Using the largest metalla-rectangles, the stability constants of association are comprised between 5.2 × 104 and 6.9 × 104 M-1 which implies a strong affinity between the metalla-rectangle and the aromatic molecule but suggests no selectivity by the metalla-rectangle among these guests.


### 3.2. Encapsulation of Guest Molecules with Metalla-Prisms and Metalla-Cubes

As demonstrated with the metalla-rectangles, the presence of a cavity cannot warrant encapsulation of guest molecules. This is also true with metalla-prisms and metalla-cubes. Indeed, the smallest metalla-prism capable of encapsulating guest molecules was [Ru6(p-cymene)6(tpt)2(dhbq)3]6+ with a Ru⋯Ru separation of approximately 7.6 Å within the dhbq clip and a Ru⋯Ru separation of approximately 13.2 Å through the tpt panels. This cavity allows the encapsulation of triphenylene derivatives such as hexahydroxytriphenylene and hexamethoxytriphenylene, thus forming carceplex systems [76]. During these studies, we showed for the first time that the formation of these carceplex systems can easily be monitored by one-dimensional 1H-ROESY NMR experiments. Using the ROESY experiment, estimation of the different host-guest H⋯H separations could be established from the NMR data and the H⋯H distances found by NMR were in good agreement with the values obtained by single-crystal X-ray structure analysis [76].In 2008, we reported the ability of the ruthenium metalla-prisms [Ru6(p-cymene)6(tpt)2(dhbq)3]6+, [Ru6(p-cymene)6(tpt)2(dchq)3]6+, [Ru6(C6Me6)6(tpt)2(dhbq)3]6+, and [Ru6(C6Me6)6(tpt)2(dchq)3]6+ to permanently encapsulate square planar complexes and aromatic molecules [77] (Figure 14). The assembly could be achieved in the presence of large aromatic molecules such as pyrene, fluoranthene, benzo[e]pyrene, triphenylene, or coronene to give the corresponding inclusion systems [aromatic⊂prism]6+. Interestingly, pyrene could be encapsulated even if it contains a functionalized aliphatic substituent; in this case the aromatic moiety was included in the cage, while the functionalized side arm stood out. These findings opened a simple and straightforward access to the preparation of arene ruthenium metalla-prismatic cages able to encapsulate aromatic and functionalized aromatic molecules, thus demonstrating that they will find applications as drug delivery containers [77].Figure 14
Synthesis of metalla-prisms, [Ru6(p-cymene)6(tpt)2(dhbq)3]6+, [Ru6(p-cymene)6(tpt)2(dchq)3]6+, [Ru6(hexamethylbenzene)6(tpt)2(dhbq)3]6+, and [Ru6(hexamethylbenzene)6(tpt)2(dchq)3]6+, and molecular structure of the corresponding host-guest systems, adapted from [78].Subsequently, the capacity to permanently encapsulate large planar molecules as well as to allow host-guest chemistry to take place with smaller aromatic molecules was demonstrated with a slightly more spacious metalla-prism [Ru6(p-cymene)6(tpt)2(dhnq)3]6+ (Figure 15) [78]. All stability constants of association (Ka) determined by NMR spectroscopy were found to be larger than 2.4 × 104 M-1 for the host-guest systems with pyrene, phenanthrene, and (pyren-1-ylmethyl)amine. On the other hand, using Pt(acac)2 and triphenylene, only carceplexes were obtained.Figure 15
Molecular structure of the metalla-prism [Ru6(p-cymene)6(tpt)2(dhnq)3]6+, adapted from [79].These studies were extended to two larger cationic metalla-prisms, namely, [Ru6(p-cymene)6(tpt)2(doaq)3]6+ and [Ru6(p-cymene)6(tpt)2(dotq)3]6+ (Figure 16). The addition of one equivalent of phenanthrene, pyrene, triphenylene, or coronene during the preparation of the metalla-prism leads to the direct encapsulation of the aromatic molecule [79]. Interestingly, both metalla-prisms exhibited host-guest properties in solution only in the presence of small aromatic molecules (phenanthrene and pyrene). However, for larger aromatic molecules (triphenylene and coronene) capable of fitting into the cavity but too large to exit the portal of the cage, permanent encapsulation was observed, thus giving rise to stable carceplex systems.Figure 16
Molecular structures of the metalla-prisms [Ru6(p-cymene)6(tpt)2(doaq)3]6+ and [Ru6(p-cymene)6(tpt)2(dotq)3]6+, adapted from [80].


## 3.1. Encapsulation of Guest Molecules with Metalla-Rectangles

The ability of the metalla-rectangles [Ru4(p-cymene)4(N∩N)2(dhnq)2]4+ (N∩N = pyrazine, 4,4′-bipyridine, and 1,2-bis(4-pyridyl)ethylene; dhnq = 5,8-dihydroxy-1,4-naphthoquinonato) to host pyrene in solution has been investigated (Figure 12) [72]. In the case of the smallest metalla-rectangle [Ru4(p-cymene)4(pyrazine)2(dhnq)2]4+, no interaction between the metalla-rectangle and pyrene was observed. However, in the case of the medium-sized metalla-rectangle [Ru4(p-cymene)4(4,4′-bipyridine)2(dhnq)2]4+, interactions occurred on the outside of the rectangular assembly, while in the larger metalla-rectangle [Ru4(p-cymene)4(1,2-bis(4-pyridyl)ethylene)2(dhnq)2]4+, the pyrene molecule was found inside the hydrophobic cavity of the metalla-rectangle, thus giving rise to a host-guest system.Molecular structure of the arene ruthenium metalla-rectangles, [Ru4(p-cymene)4(pyrazine)2(dhnq)2]4+ (a), [Ru4(p-cymene)4(4,4′-bipyridine)2(dhnq)2]4+(b), and [Ru4(p-cymene)4(1,2-bis(4-pyridyl))(ethylene)2(dhnq)2]4+(c), adapted from [72].
(a)
(b)
(c)During this study, we exploited two-dimensional1H-DOSY NMR experiments to gain further insight into the nature of the interactions involved between pyrene and the metalla-rectangles. Diffusion-ordered spectroscopy (DOSY) is a well-established NMR technique that allows separating the NMR signals of different species according to their diffusion coefficient [73, 74]. From the DOSY spectra, it is evidenced that the diffusion constants of pyrene and of the medium-sized metalla-rectangle [Ru4(p-cymene)4(4,4′-bipyridine)2(dhnq)2]4+ are unaffected, when mixed together. Therefore, the chemical shifts observed for the host and the guest were attributed to outer interactions. On the other hand, in the case of the larger metalla-rectangle [Ru4(p-cymene)4(1,2-bis(4-pyridyl)ethylene)2(dhnq)2]4+, the interaction with pyrene is totally different. The DOSY spectra show that for a 1 : 1 mixture of pyrene and [Ru4(p-cymene)4(1,2-bis(4-pyridyl) ethylene)2(dhnq)2]4+ both diffusion coefficients are almost equivalent at about 6.3×10-10 m2·s-1 (Figure 13). Consequently, the pyrene appears to be located in the hydrophobic cavity of [Ru4(p-cymene)4(1,2-bis(4-pyridyl)ethylene)2(dhnq)2]4+, which is in accordance with the strong chemical shift observed for both species in the 1H NMR spectra.Figure 13
DOSY1H NMR spectra of pyrene, of the metalla-rectangle [Ru4(p-cymene)4(1,2-bis(4-pyridyl)ethylene)2(dhnq)2]4+, and of additional amounts of pyrene (1 to 3 equivalent) to a 4.0 mM solution of [Ru4(p-cymene)4(1,2-bis(4-pyridyl) ethylene)2(dhnq)2]4+dissolved in CD3CN, adapted from [72].We have subsequently extended this first study to three other guest molecules, anthracene, perylene, and coronene, and used 9,10-dihydroxy-1,4-anthraquinonato (dhaq) and 6,11-dihydroxynaphthacene-5,12-dionato (dhtq) bridging ligands in addition to 5,8-dihydroxy-1,4-naphthoquinonato (dhnq) [75]. As observed with the metalla-rectangles [Ru4(p-cymene)4(pyrazine)2(dhnq)2]4+, [Ru4(p-cymene)4(4,4′-bipyridine)2(dhnq)2]4+, and [Ru4(p-cymene)4(1,2-bis(4-pyridyl) ethylene)2(dhnq)2]4+, the largest metalla-rectangles incorporating 1,2-bis(4-pyridyl) ethylene linkers are able to host an anthracene, pyrene, perylene, or coronene molecule in their cavity, while the medium-sized metalla-rectangles incorporating 4,4′-bipyridine linkers are only able to encapsulate anthracene. However, out-of-cavity interactions are observed between these 4,4′-bipyridine-containing metalla-rectangles and pyrene, perylene, or coronene. In contrast, the small pyrazine-containing metalla-rectangles showed no interaction in solution with this series of planar aromatic molecules. Using the largest metalla-rectangles, the stability constants of association are comprised between 5.2 × 104 and 6.9 × 104 M-1 which implies a strong affinity between the metalla-rectangle and the aromatic molecule but suggests no selectivity by the metalla-rectangle among these guests.


## 3.2. Encapsulation of Guest Molecules with Metalla-Prisms and Metalla-Cubes

As demonstrated with the metalla-rectangles, the presence of a cavity cannot warrant encapsulation of guest molecules. This is also true with metalla-prisms and metalla-cubes. Indeed, the smallest metalla-prism capable of encapsulating guest molecules was [Ru6(p-cymene)6(tpt)2(dhbq)3]6+ with a Ru⋯Ru separation of approximately 7.6 Å within the dhbq clip and a Ru⋯Ru separation of approximately 13.2 Å through the tpt panels. This cavity allows the encapsulation of triphenylene derivatives such as hexahydroxytriphenylene and hexamethoxytriphenylene, thus forming carceplex systems [76]. During these studies, we showed for the first time that the formation of these carceplex systems can easily be monitored by one-dimensional 1H-ROESY NMR experiments. Using the ROESY experiment, estimation of the different host-guest H⋯H separations could be established from the NMR data and the H⋯H distances found by NMR were in good agreement with the values obtained by single-crystal X-ray structure analysis [76].In 2008, we reported the ability of the ruthenium metalla-prisms [Ru6(p-cymene)6(tpt)2(dhbq)3]6+, [Ru6(p-cymene)6(tpt)2(dchq)3]6+, [Ru6(C6Me6)6(tpt)2(dhbq)3]6+, and [Ru6(C6Me6)6(tpt)2(dchq)3]6+ to permanently encapsulate square planar complexes and aromatic molecules [77] (Figure 14). The assembly could be achieved in the presence of large aromatic molecules such as pyrene, fluoranthene, benzo[e]pyrene, triphenylene, or coronene to give the corresponding inclusion systems [aromatic⊂prism]6+. Interestingly, pyrene could be encapsulated even if it contains a functionalized aliphatic substituent; in this case the aromatic moiety was included in the cage, while the functionalized side arm stood out. These findings opened a simple and straightforward access to the preparation of arene ruthenium metalla-prismatic cages able to encapsulate aromatic and functionalized aromatic molecules, thus demonstrating that they will find applications as drug delivery containers [77].Figure 14
Synthesis of metalla-prisms, [Ru6(p-cymene)6(tpt)2(dhbq)3]6+, [Ru6(p-cymene)6(tpt)2(dchq)3]6+, [Ru6(hexamethylbenzene)6(tpt)2(dhbq)3]6+, and [Ru6(hexamethylbenzene)6(tpt)2(dchq)3]6+, and molecular structure of the corresponding host-guest systems, adapted from [78].Subsequently, the capacity to permanently encapsulate large planar molecules as well as to allow host-guest chemistry to take place with smaller aromatic molecules was demonstrated with a slightly more spacious metalla-prism [Ru6(p-cymene)6(tpt)2(dhnq)3]6+ (Figure 15) [78]. All stability constants of association (Ka) determined by NMR spectroscopy were found to be larger than 2.4 × 104 M-1 for the host-guest systems with pyrene, phenanthrene, and (pyren-1-ylmethyl)amine. On the other hand, using Pt(acac)2 and triphenylene, only carceplexes were obtained.Figure 15
Molecular structure of the metalla-prism [Ru6(p-cymene)6(tpt)2(dhnq)3]6+, adapted from [79].These studies were extended to two larger cationic metalla-prisms, namely, [Ru6(p-cymene)6(tpt)2(doaq)3]6+ and [Ru6(p-cymene)6(tpt)2(dotq)3]6+ (Figure 16). The addition of one equivalent of phenanthrene, pyrene, triphenylene, or coronene during the preparation of the metalla-prism leads to the direct encapsulation of the aromatic molecule [79]. Interestingly, both metalla-prisms exhibited host-guest properties in solution only in the presence of small aromatic molecules (phenanthrene and pyrene). However, for larger aromatic molecules (triphenylene and coronene) capable of fitting into the cavity but too large to exit the portal of the cage, permanent encapsulation was observed, thus giving rise to stable carceplex systems.Figure 16
Molecular structures of the metalla-prisms [Ru6(p-cymene)6(tpt)2(doaq)3]6+ and [Ru6(p-cymene)6(tpt)2(dotq)3]6+, adapted from [80].


## 4. Drug Delivery

### 4.1. Encapsulation and Release of Bioactive Molecules

The molecular recognition properties coupled with the promising biological activity of ruthenium assemblies suggested that these compounds could potentially act as drug delivery vectors. We have first reported complex-in-a-complex systems, in which platinum or palladium acetyl acetate complexes were encapsulated in the cavity of the metalla-prism (Figure17) [80]. The guest complexes by themselves are biologically inactive due to their insolubility in aqueous media. The metalla-prism and host-guest systems, which are on the other hand both water-soluble, are shown to possess high cytotoxic activity [80]. This and subsequent studies to monitor thein vitro release of fluorescent pyrene-based guest molecules, whose fluorescence is suppressed when encapsulated within the metalla-assembly, have shown that the water-soluble metalla-prism is capable of being internalized by cancerous cells and subsequently broken down to release the hydrophobic guest molecules, which would otherwise have been unable to enter the cells [81].Figure 17
Molecular structure of the complex-in-a-complex system [Pt(acac)2⊂Ru6(p-cymene)6(tpt)2(dhbq)3]6+, adapted from [81].This first success has encouraged us to further investigate these metalla-prisms as drug delivery vectors. Monosubstituted pyrenyl derivatives were also encapsulated in the metalla-prism [Ru6(p-cymene)6(tpt)2(dhbq)3]6+ and the drug delivery ability of the resulting host-guests systems was evaluated [81]. The pyrenyl derivatives included biologically relevant structures, especially pyrenyl ethacrynic, a derivate of ethacrynic acid, and 2-(pyren-1-ylmethylcarbamoyl) phenyl acetate, the corresponding acid chloride of aspirin (Figure 18). Ethacrynic acid is notably known as an inhibitor of glutathione transferase (GST) [83, 84]. The host-guest nature of these systems with the pyrenyl moiety being encapsulated in the hydrophobic cavity of the cage with the functional groups pointing outwards was confirmed by NMR spectroscopy. All the host-guest systems are more cytotoxic than the empty cage alone, the most active carceplex being an order of magnitude more cytotoxic. Thisin vitrostudy revealed that the nature of the pyrenyl derivative strongly influences the cytotoxicity of the system and consequently confirms that these metalla-cages offer a strategy to deliver biologically relevant molecules to cancer cells [81].Figure 18
Pyrenyl derivatives encapsulated in the metalla-prism [Ru6(p-cymene)6(tpt)2(dhbq)3]6+ (a= 1-pyrenebutyric acid,b= 1-pyrenebutanol,c= 1-pyrenemethylamine,d= 1-pyrenemethylbutanoate,e= 1-(4,6-dichloro-1,3,5-triazin-2-yl) pyrene,f= N-hexadecylpyrene-1-sulfonamide,g= pyrenyl ethacrynic amide,h= 2-(pyren-1-ylmethylcarbamoyl)phenyl acetate) and a disubstituted pyrenyl derivative (i= 1,8-bis(3-methyl-butyn-1-yl-3-ol)pyrene).Subsequently, the host-guest properties of a series of larger metalla-prisms [Ru6(p-cymene)6(tpt)2(dhnq)3]6+, [Ru6(p-cymene)6(tpt)2(doaq)3]6+, and [Ru6(p-cymene)6(tpt)2(dotq)3]6+ having similar sized cavities but different portal sizes have been studied [82]. These metalla-prisms are able to encapsulate the fluorescent guest molecule 1-(4,6-dichloro-1,3,5-triazin-2-yl)pyrene (pyrene-R) and the complex Pd(acac)2. Remarkably, the rate of release of the guest molecule is found to depend on the portal size of the host, as shown by flow cytometric analysis of cells incubated with the pyrene-R encapsulated in the three metalla-prisms (Figure 19). It can be seen that the ratio between cell fluorescence and the intracellular ruthenium concentration varies as a function of the metalla-cage used to entrap the pyrene-R fluorophore.Figure 19
Fluorescence recorded by flow cytometry of the metalla-prism [Ru6(p-cymene)6(tpt)2(dhnq)3]6+(A) and of 1-(4,6-dichloro-1,3,5-triazin-2-yl)pyrene encapsulated in the metalla-prisms [Ru6(p-cymene)6(tpt)2(dhnq)3]6+(B), [Ru6(p-cymene)6(tpt)2(doaq)3]6+ (C), and [Ru6(p-cymene)6(tpt)2(dotq)3]6+(D), indicating pyrene-R released from the host (blue bars). Ruthenium uptake determined by ICP-MS is also shown (red bars). A2780 cells were incubated with the metalla-prism [Ru6(p-cymene)6(tpt)2(dhnq)3]6+and with pyrene-R encapsulated in the three metalla-prisms at 2 μm concentration for 24 h, adapted from [82].Nevertheless, the uptake of ruthenium into the cells determined by inductively coupled plasma mass spectroscopy (ICP-MS) reveals that all compounds enter the cells to essentially the same extent, suggesting a similar uptake mechanism for the three supramolecular complexes. Furthermore, confocal microscopy showed that the pyrene-R stains vesicle like cytoplasmic organelles and accumulates in the intracellular vesicle of the cell endocytotic system. A comparison between the fluorescence patterns of pyrene-R with known fluorescent reporters showed that the free pyrene-R does not enter lysosomal compartments and does not accumulate in the endocytic compartments, which suggest that pyrene-R accumulates in the endocytic rather than the lytic compartments. Through the use of specific inhibitors and fluorescent reporters, we have shown that endocytosis is probably involved in the uptake and leads to the accumulation of the guest molecule in intracellular organelles.Lately, we have been interested in the synthesis of host-guest systems composed of water-soluble arene ruthenium metalla-prisms and pyrenyl-functionalized dendrimers (Figure20) [85–87]. Dendrimers with defined structures are well established and have been studied as potential drug delivery vectors [26]. In particular, water-soluble and biodegradable dendrimers are quite promising, as they have been shown to improve drug solubility, to increase drug circulation time, and to prolong drug residence in tumors.Figure 20
Encapsulation of pyrenyl-functionalized dendrimers into the cavity of the metalla-prism [Ru6(p-cymene)6(tpt)2(dhnq)3]6+.We have first reported the synthesis and cytotoxicity of host-guest systems composed of water-soluble arene ruthenium metalla-prisms and pyrenyl-functionalized dendrimers [86]. These studies showed that the host-guest systems inhibit the growth of both sensitive and cisplatin resistant cancer cells (A2780 and A2780cisR) with IC50 values significantly lower than those of the empty metalla-prisms. The dendrimers show no cytotoxic effects on these cell lines, presumably owing to their poor solubility in water. However, for the first time, this study has shown that metalla-cage host systems are able to deliver large hydrophobic guest molecules into cancer cells.Subsequently, we have synthesized different generations of biodegradable water-soluble dendrimers based on the bis-MPA monomer functionalized by a pyrenyl unit [87]. The aromatic part of the pyrenyl-functionalized dendrimers could be encapsulated into the hydrophobic cavity of the metalla-prisms [Ru6(p-cymene)6(tpt)2(dhnq)3]6+ and [Ru6(p-cymene)6(tpt)2(dotq)3]6+ (Figure 21). Remarkably, the cytotoxicity of the resulting host-guest systems (IC50 values ranging from 0.3 to 4.1 μM) was considerably greater than that of the individual hosts (IC50 values 3.1 and 4.1 μM) and guests (IC50 values 5.9 and 8.7 μM). The enhanced cytotoxicity of the host-guest systems compared to the guest or host alone implies that intact host-guest systems are entering the cells together and are responsible for the overall cytotoxicity observed. Notably, the host-guest system is also quite stable in biological media. These features, combined with the potential of water-soluble dendrimers guests within ruthenium metalla-prism hosts to preferentially accumulate in tumors, make them interesting candidates for further study and development.Figure 21
Biodegradable water-soluble dendrimers encapsulated in the cavity of the metalla-prisms [Ru6(p-cymene)6(tpt)2(dhnq)3]6+and [Ru6(p-cymene)6(tpt)2(dotq)3]6+[85].


### 4.2. Encapsulation and Release of Photosensitizers

Photodynamic therapy (PDT) of cancer is a very powerful and efficient method to treat diseases [88, 89], but the treatment is potentially hampered by several aspects, one of the most problematical being the chemical nature of the photosensitizers [90]. Indeed, photosensitizers such as porphyrins and phthalocyanines are in general poorly water-soluble, unless highly substituted with hydrophilic groups, and exhibit generally a low selectivity for cancer cells.The development of vector-mediated selective drug delivery strategies is a major challenge in PDT. As such, encapsulation of the photosensitizer within the hydrophobic cavity of water-soluble carriers provides an elegant strategy to transport photosensitizers in aqueous media.Using the metalla-prism [Ru6(p-cymene)6(tpt)2(dhnq)3]6+ and a larger octanuclear metalla-cube [Ru8(p-cymene)(dhnq)4(tpvb)2]8+ (tbpv = 1,2,4,5-tetrakis {2-(4-pyridyl) vinylbenzene)} (Figure 22), both water-soluble, we could demonstrate that porphin was encapsulated in the metalla-cages and subsequently delivered intracellularly [91–93]. As expected, the release of porphin is higher for the larger cubic cage as compared to the smaller prismatic cage. Interestingly, both systems display hypochromism properties toward the photosensitizer when encapsulated inside the cavity, resulting in the absence of phototoxic effect when inside the cavity. This ability demonstrates that the cages can be considered as powerful tools for new photodynamic strategies.Figure 22
Encapsulation of porphin into the cavities of the metalla-prism [Ru6(p-cymene)6(tpt)2(dhnq)3]6+and the larger octanuclear metalla-cube [Ru8(p-cymene)(dhnq)4(tpvb)2]8+.Subsequently, we have reported an extension of this work by studying the host-guest properties of the metalla-prisms [Ru6(p-cymene)6(tris-pvb)2(OO∩OO)3]6+, OO∩OO = oxalato, dhbq, dcbq, dhnq, doaq, and donq (Figure 23), and of the metalla-cubes [Ru8(p-cymene)8(NN∩NN)2(OO∩OO)4]8+ (NN∩NN = 1,2,4,5-tetrakis{2-(pyridin-4-yl)vinyl}benzene, 1,2,4,5-tetrakis{2-(pyridin-4-yl)ethynyl}benzene), OO∩OO = oxalato, dhbq, dcbq, dhnq, doaq, and donq) (Figure 24) [91]. The photosensitizers porphin, phthalocyanine, and Zn-phthalocyanine present during the synthesis of these metalla-cages are encapsulated in several of these arene ruthenium complexes to give photosensitizer-encapsulated systems. NMR analysis indicates that the guest photosensitizers are completely encapsulated in two of these metalla-cages, while in three other ruthenium cages the NMR spectra reveal an equilibrium between empty and filled metalla-cages.Figure 23
Encapsulation of various photosensitizers into the cavities of the metalla-prisms [Ru6(p-cymene)6(tris-pvb)2(μ2-Cl)6]6+ and [Ru6(p-cymene)6(tris-pvb)2(OO∩OO)3]6+, OO∩OO = oxalato, dhbq, dcbq, dhnq, doaq, and donq, adapted from [94].Figure 24
Encapsulation of various photosensitizers into the cavities of the cages [Ru8(p-cymene)8(NN∩NN)2(OO∩OO)4]8+, NN∩NN = 1,2,4,5-tetrakis{2-(pyridin-4-yl)vinyl}benzene, 1,2,4,5-tetrakis{2-(pyridin-4-yl)ethynyl}benzene), OO∩OO = oxalato, dhbq, dcbq, dhnq, doaq, and donq, adapted from [94].


## 4.1. Encapsulation and Release of Bioactive Molecules

The molecular recognition properties coupled with the promising biological activity of ruthenium assemblies suggested that these compounds could potentially act as drug delivery vectors. We have first reported complex-in-a-complex systems, in which platinum or palladium acetyl acetate complexes were encapsulated in the cavity of the metalla-prism (Figure17) [80]. The guest complexes by themselves are biologically inactive due to their insolubility in aqueous media. The metalla-prism and host-guest systems, which are on the other hand both water-soluble, are shown to possess high cytotoxic activity [80]. This and subsequent studies to monitor thein vitro release of fluorescent pyrene-based guest molecules, whose fluorescence is suppressed when encapsulated within the metalla-assembly, have shown that the water-soluble metalla-prism is capable of being internalized by cancerous cells and subsequently broken down to release the hydrophobic guest molecules, which would otherwise have been unable to enter the cells [81].Figure 17
Molecular structure of the complex-in-a-complex system [Pt(acac)2⊂Ru6(p-cymene)6(tpt)2(dhbq)3]6+, adapted from [81].This first success has encouraged us to further investigate these metalla-prisms as drug delivery vectors. Monosubstituted pyrenyl derivatives were also encapsulated in the metalla-prism [Ru6(p-cymene)6(tpt)2(dhbq)3]6+ and the drug delivery ability of the resulting host-guests systems was evaluated [81]. The pyrenyl derivatives included biologically relevant structures, especially pyrenyl ethacrynic, a derivate of ethacrynic acid, and 2-(pyren-1-ylmethylcarbamoyl) phenyl acetate, the corresponding acid chloride of aspirin (Figure 18). Ethacrynic acid is notably known as an inhibitor of glutathione transferase (GST) [83, 84]. The host-guest nature of these systems with the pyrenyl moiety being encapsulated in the hydrophobic cavity of the cage with the functional groups pointing outwards was confirmed by NMR spectroscopy. All the host-guest systems are more cytotoxic than the empty cage alone, the most active carceplex being an order of magnitude more cytotoxic. Thisin vitrostudy revealed that the nature of the pyrenyl derivative strongly influences the cytotoxicity of the system and consequently confirms that these metalla-cages offer a strategy to deliver biologically relevant molecules to cancer cells [81].Figure 18
Pyrenyl derivatives encapsulated in the metalla-prism [Ru6(p-cymene)6(tpt)2(dhbq)3]6+ (a= 1-pyrenebutyric acid,b= 1-pyrenebutanol,c= 1-pyrenemethylamine,d= 1-pyrenemethylbutanoate,e= 1-(4,6-dichloro-1,3,5-triazin-2-yl) pyrene,f= N-hexadecylpyrene-1-sulfonamide,g= pyrenyl ethacrynic amide,h= 2-(pyren-1-ylmethylcarbamoyl)phenyl acetate) and a disubstituted pyrenyl derivative (i= 1,8-bis(3-methyl-butyn-1-yl-3-ol)pyrene).Subsequently, the host-guest properties of a series of larger metalla-prisms [Ru6(p-cymene)6(tpt)2(dhnq)3]6+, [Ru6(p-cymene)6(tpt)2(doaq)3]6+, and [Ru6(p-cymene)6(tpt)2(dotq)3]6+ having similar sized cavities but different portal sizes have been studied [82]. These metalla-prisms are able to encapsulate the fluorescent guest molecule 1-(4,6-dichloro-1,3,5-triazin-2-yl)pyrene (pyrene-R) and the complex Pd(acac)2. Remarkably, the rate of release of the guest molecule is found to depend on the portal size of the host, as shown by flow cytometric analysis of cells incubated with the pyrene-R encapsulated in the three metalla-prisms (Figure 19). It can be seen that the ratio between cell fluorescence and the intracellular ruthenium concentration varies as a function of the metalla-cage used to entrap the pyrene-R fluorophore.Figure 19
Fluorescence recorded by flow cytometry of the metalla-prism [Ru6(p-cymene)6(tpt)2(dhnq)3]6+(A) and of 1-(4,6-dichloro-1,3,5-triazin-2-yl)pyrene encapsulated in the metalla-prisms [Ru6(p-cymene)6(tpt)2(dhnq)3]6+(B), [Ru6(p-cymene)6(tpt)2(doaq)3]6+ (C), and [Ru6(p-cymene)6(tpt)2(dotq)3]6+(D), indicating pyrene-R released from the host (blue bars). Ruthenium uptake determined by ICP-MS is also shown (red bars). A2780 cells were incubated with the metalla-prism [Ru6(p-cymene)6(tpt)2(dhnq)3]6+and with pyrene-R encapsulated in the three metalla-prisms at 2 μm concentration for 24 h, adapted from [82].Nevertheless, the uptake of ruthenium into the cells determined by inductively coupled plasma mass spectroscopy (ICP-MS) reveals that all compounds enter the cells to essentially the same extent, suggesting a similar uptake mechanism for the three supramolecular complexes. Furthermore, confocal microscopy showed that the pyrene-R stains vesicle like cytoplasmic organelles and accumulates in the intracellular vesicle of the cell endocytotic system. A comparison between the fluorescence patterns of pyrene-R with known fluorescent reporters showed that the free pyrene-R does not enter lysosomal compartments and does not accumulate in the endocytic compartments, which suggest that pyrene-R accumulates in the endocytic rather than the lytic compartments. Through the use of specific inhibitors and fluorescent reporters, we have shown that endocytosis is probably involved in the uptake and leads to the accumulation of the guest molecule in intracellular organelles.Lately, we have been interested in the synthesis of host-guest systems composed of water-soluble arene ruthenium metalla-prisms and pyrenyl-functionalized dendrimers (Figure20) [85–87]. Dendrimers with defined structures are well established and have been studied as potential drug delivery vectors [26]. In particular, water-soluble and biodegradable dendrimers are quite promising, as they have been shown to improve drug solubility, to increase drug circulation time, and to prolong drug residence in tumors.Figure 20
Encapsulation of pyrenyl-functionalized dendrimers into the cavity of the metalla-prism [Ru6(p-cymene)6(tpt)2(dhnq)3]6+.We have first reported the synthesis and cytotoxicity of host-guest systems composed of water-soluble arene ruthenium metalla-prisms and pyrenyl-functionalized dendrimers [86]. These studies showed that the host-guest systems inhibit the growth of both sensitive and cisplatin resistant cancer cells (A2780 and A2780cisR) with IC50 values significantly lower than those of the empty metalla-prisms. The dendrimers show no cytotoxic effects on these cell lines, presumably owing to their poor solubility in water. However, for the first time, this study has shown that metalla-cage host systems are able to deliver large hydrophobic guest molecules into cancer cells.Subsequently, we have synthesized different generations of biodegradable water-soluble dendrimers based on the bis-MPA monomer functionalized by a pyrenyl unit [87]. The aromatic part of the pyrenyl-functionalized dendrimers could be encapsulated into the hydrophobic cavity of the metalla-prisms [Ru6(p-cymene)6(tpt)2(dhnq)3]6+ and [Ru6(p-cymene)6(tpt)2(dotq)3]6+ (Figure 21). Remarkably, the cytotoxicity of the resulting host-guest systems (IC50 values ranging from 0.3 to 4.1 μM) was considerably greater than that of the individual hosts (IC50 values 3.1 and 4.1 μM) and guests (IC50 values 5.9 and 8.7 μM). The enhanced cytotoxicity of the host-guest systems compared to the guest or host alone implies that intact host-guest systems are entering the cells together and are responsible for the overall cytotoxicity observed. Notably, the host-guest system is also quite stable in biological media. These features, combined with the potential of water-soluble dendrimers guests within ruthenium metalla-prism hosts to preferentially accumulate in tumors, make them interesting candidates for further study and development.Figure 21
Biodegradable water-soluble dendrimers encapsulated in the cavity of the metalla-prisms [Ru6(p-cymene)6(tpt)2(dhnq)3]6+and [Ru6(p-cymene)6(tpt)2(dotq)3]6+[85].


## 4.2. Encapsulation and Release of Photosensitizers

Photodynamic therapy (PDT) of cancer is a very powerful and efficient method to treat diseases [88, 89], but the treatment is potentially hampered by several aspects, one of the most problematical being the chemical nature of the photosensitizers [90]. Indeed, photosensitizers such as porphyrins and phthalocyanines are in general poorly water-soluble, unless highly substituted with hydrophilic groups, and exhibit generally a low selectivity for cancer cells.The development of vector-mediated selective drug delivery strategies is a major challenge in PDT. As such, encapsulation of the photosensitizer within the hydrophobic cavity of water-soluble carriers provides an elegant strategy to transport photosensitizers in aqueous media.Using the metalla-prism [Ru6(p-cymene)6(tpt)2(dhnq)3]6+ and a larger octanuclear metalla-cube [Ru8(p-cymene)(dhnq)4(tpvb)2]8+ (tbpv = 1,2,4,5-tetrakis {2-(4-pyridyl) vinylbenzene)} (Figure 22), both water-soluble, we could demonstrate that porphin was encapsulated in the metalla-cages and subsequently delivered intracellularly [91–93]. As expected, the release of porphin is higher for the larger cubic cage as compared to the smaller prismatic cage. Interestingly, both systems display hypochromism properties toward the photosensitizer when encapsulated inside the cavity, resulting in the absence of phototoxic effect when inside the cavity. This ability demonstrates that the cages can be considered as powerful tools for new photodynamic strategies.Figure 22
Encapsulation of porphin into the cavities of the metalla-prism [Ru6(p-cymene)6(tpt)2(dhnq)3]6+and the larger octanuclear metalla-cube [Ru8(p-cymene)(dhnq)4(tpvb)2]8+.Subsequently, we have reported an extension of this work by studying the host-guest properties of the metalla-prisms [Ru6(p-cymene)6(tris-pvb)2(OO∩OO)3]6+, OO∩OO = oxalato, dhbq, dcbq, dhnq, doaq, and donq (Figure 23), and of the metalla-cubes [Ru8(p-cymene)8(NN∩NN)2(OO∩OO)4]8+ (NN∩NN = 1,2,4,5-tetrakis{2-(pyridin-4-yl)vinyl}benzene, 1,2,4,5-tetrakis{2-(pyridin-4-yl)ethynyl}benzene), OO∩OO = oxalato, dhbq, dcbq, dhnq, doaq, and donq) (Figure 24) [91]. The photosensitizers porphin, phthalocyanine, and Zn-phthalocyanine present during the synthesis of these metalla-cages are encapsulated in several of these arene ruthenium complexes to give photosensitizer-encapsulated systems. NMR analysis indicates that the guest photosensitizers are completely encapsulated in two of these metalla-cages, while in three other ruthenium cages the NMR spectra reveal an equilibrium between empty and filled metalla-cages.Figure 23
Encapsulation of various photosensitizers into the cavities of the metalla-prisms [Ru6(p-cymene)6(tris-pvb)2(μ2-Cl)6]6+ and [Ru6(p-cymene)6(tris-pvb)2(OO∩OO)3]6+, OO∩OO = oxalato, dhbq, dcbq, dhnq, doaq, and donq, adapted from [94].Figure 24
Encapsulation of various photosensitizers into the cavities of the cages [Ru8(p-cymene)8(NN∩NN)2(OO∩OO)4]8+, NN∩NN = 1,2,4,5-tetrakis{2-(pyridin-4-yl)vinyl}benzene, 1,2,4,5-tetrakis{2-(pyridin-4-yl)ethynyl}benzene), OO∩OO = oxalato, dhbq, dcbq, dhnq, doaq, and donq, adapted from [94].


## 5. Reactivity of Arene Ruthenium Metalla-Prisms

As mentioned in the previous parts, the expected function of these ruthenium metalla-assemblies is the selective release of an encapsulated drug in cancer cells. In principle, carceplexes as well as host-guest systems could be used to achieve this goal. However, both systems have inherent advantages and drawbacks that should be first considered. In carceplex systems, the guest molecule is expected to remain encapsulated in the metalla-assembly until the host-guest system reaches its final target. The drawbacks of carceplex systems are therefore directly related to the stability of the metalla-assembly in biological media. If the stability of the metalla-assembly is insufficient, the guest molecule will be released in an uncontrolled manner within the human body and at sites where the guest molecule is not necessarily needed, leading potentially to a general toxicity and to undesired side effects. Likewise, if the stability of the metalla-assembly is excessively high, the selective release of an encapsulated drug at the desired sites might last too long or may even never occur if the metalla-assembly remains intact.The advantages and drawbacks of host-guest systems are similar to those of the carceplex systems, with the additional problem of controlling the exchange rate of the encapsulated molecule. Indeed, if the rate of exchange is too fast, reactions between the encapsulated molecule and the body’s molecular components (proteins, amino acids, and lipids) cannot be avoided and most importantly cannot be controlled. Furthermore, because a guest molecule might well react with its surroundings within human bodies, the probability that the guest molecule reenters into the cavity of the metalla-assembly is significantly reduced. On the contrary, if the rate of exchange is too slow, the selective release of an encapsulated drug in cancer cells will be excessively long or even be completely suppressed if the exchange rate approaches zero.According to our recent findings, it turns out that controlling the exchange rate of a guest molecule encapsulated in a ruthenium metalla-assembly is a demanding task. Indeed, to obtain a suitable exchange rate between the assembly and the guest molecule, that is, to design a host-guest system that would be able to release the target molecule at the desired site and at the desired time interval, a fine tuning of both structures of the metalla-assembly and of the guest molecule is required. For those reasons, we believe that, for selectively releasing an encapsulated drug in cancer cells, carceplex systems appear more appropriate than host-guest systems. Thus, in order to gain new insights into uptake, release of the guest, and the other biological processes, we have started to monitor reactions of various ruthenium metalla-prisms with biological targets. Since all host-guest systems studied so far are more cytotoxic than the empty assemblies themselves, these studies were also useful for understanding their possible mechanisms of action.


### 5.1. Reactivity against Amino Acids and Proteins

Various experiments were undertaken in our groups to establish the nature of the species that are effectively transported into the cell, possible mechanisms of detoxification, and further information on the cellular target that may be related to the antitumor activity of the metalla-prisms. Our first results showed that the metalla-prism [Ru6(p-cymene)6(tpt)2(dhbq)3]6+ specifically interacts with the thiol groups of Cys and GSH and with the basic side chains of His, Lys, and Arg but remains surprisingly inert upon the addition of Met [94]. The results suggest that the reaction of the metalla-prism [Ru6(p-cymene)6(tpt)2(dhbq)3]6+ with Arg, His, and Lys takes place within a few minutes with quantitative release of the tpt and dhbq ligands. As a plausible mechanism, Arg, His, and Lys may first coordinate to the ruthenium atom via one of the basic groups of the side chain inducing the quantitative release of the tpt moiety. Through this first coordination, the two Ru-O bonds between the ruthenium atom and dhbq are released by the carboxylato and the amino groups of the amino acid which can act as second and third ligands, thus forming N,N,O- or N,O-chelate adducts (Figure 25).Figure 25
Suggested structures of the bi- and tridentate chelate complexes obtained from the reaction of Arg, His, and Lys with the metalla-prism [Ru6(p-cymene)6(tpt)2(dhbq)3]6+ (adapted from [94]).Interestingly, the larger metalla-prism [Ru6(p-cymene)6(tpt)2(dhnq)3]6+ appears to be significantly more stable than the metalla-prism [Ru6(p-cymene)6(tpt)2(dhbq)3]6+ and is disassembled in the presence of Arg, His, and Lys after 12 h of incubation [95]. Moreover, the reaction with His was not complete, since only 14% of His reacted after more than 1 week of incubation. Our results suggest that the metalla-prism [Ru6(p-cymene)6(tpt)2(dhnq)3]6+ which is more robust than metalla-prism [Ru6(p-cymene)6(tpt)2(dhbq)3]6+ may remain intact in the bloodstream and, therefore, may enter cancer cells undamaged.Our results obtained with these ruthenium metalla-prisms seem to corroborate a recent study by Hanif et al. [96], which suggests an inverse correlation between metallo-drug protein interaction and cytotoxicity against tumor cells. Indeed, similar to the metalla-prism [Ru6(p-cymene)6(tpt)2(dhnq)3]6+ (IC50 = 0.45 μM against A2780 cells), the metalla-prism [Ru6(p-cymene)6(tpt)2(oxalato)3]6+ (IC50 = 0.93 μM against A2780 cells) reacts relatively slowly and incompletely with biomolecules [97]. The less cytotoxic assembly (IC50 = 0.96 μM against A2780 cells), [Ru6(p-cymene)6(tpt)2(dhbq)3]6+, on the other hand, reacts rather quickly with biomolecules, and a complete and fast disassembly could be observed with Arg, His, and Lys.


### 5.2. Tuning/Controlling the Reactivity

As shown in the previous sections, ruthenium assemblies must exhibit a certain degree of stability, in order to selectively release the encapsulated drug in the cancer cells and not in the cytosol. As discussed, the stability of metalla-assemblies is a central criterion and should be sufficient; otherwise the drug might be released in an uncontrolled manner within the human body. The stability should also not be excessive; otherwise the drug might remain encapsulated and the host-guest system might spread in an uncontrolled manner within the human body.Our results suggest a connection between the linkers used and the reactivity of the resulting metalla-assembly. To further investigate this hypothesis, studies of other arene ruthenium assemblies bearing bulkier ligands such as hexamethylbenzene instead ofp-cymene, or ruthenium assemblies with more sterically hindered linkers, are currently undertaken. The possible correlation between reactivity and cytotoxicity for these new arene ruthenium assemblies and their host-guest properties will be investigated in the near future.


## 5.1. Reactivity against Amino Acids and Proteins

Various experiments were undertaken in our groups to establish the nature of the species that are effectively transported into the cell, possible mechanisms of detoxification, and further information on the cellular target that may be related to the antitumor activity of the metalla-prisms. Our first results showed that the metalla-prism [Ru6(p-cymene)6(tpt)2(dhbq)3]6+ specifically interacts with the thiol groups of Cys and GSH and with the basic side chains of His, Lys, and Arg but remains surprisingly inert upon the addition of Met [94]. The results suggest that the reaction of the metalla-prism [Ru6(p-cymene)6(tpt)2(dhbq)3]6+ with Arg, His, and Lys takes place within a few minutes with quantitative release of the tpt and dhbq ligands. As a plausible mechanism, Arg, His, and Lys may first coordinate to the ruthenium atom via one of the basic groups of the side chain inducing the quantitative release of the tpt moiety. Through this first coordination, the two Ru-O bonds between the ruthenium atom and dhbq are released by the carboxylato and the amino groups of the amino acid which can act as second and third ligands, thus forming N,N,O- or N,O-chelate adducts (Figure 25).Figure 25
Suggested structures of the bi- and tridentate chelate complexes obtained from the reaction of Arg, His, and Lys with the metalla-prism [Ru6(p-cymene)6(tpt)2(dhbq)3]6+ (adapted from [94]).Interestingly, the larger metalla-prism [Ru6(p-cymene)6(tpt)2(dhnq)3]6+ appears to be significantly more stable than the metalla-prism [Ru6(p-cymene)6(tpt)2(dhbq)3]6+ and is disassembled in the presence of Arg, His, and Lys after 12 h of incubation [95]. Moreover, the reaction with His was not complete, since only 14% of His reacted after more than 1 week of incubation. Our results suggest that the metalla-prism [Ru6(p-cymene)6(tpt)2(dhnq)3]6+ which is more robust than metalla-prism [Ru6(p-cymene)6(tpt)2(dhbq)3]6+ may remain intact in the bloodstream and, therefore, may enter cancer cells undamaged.Our results obtained with these ruthenium metalla-prisms seem to corroborate a recent study by Hanif et al. [96], which suggests an inverse correlation between metallo-drug protein interaction and cytotoxicity against tumor cells. Indeed, similar to the metalla-prism [Ru6(p-cymene)6(tpt)2(dhnq)3]6+ (IC50 = 0.45 μM against A2780 cells), the metalla-prism [Ru6(p-cymene)6(tpt)2(oxalato)3]6+ (IC50 = 0.93 μM against A2780 cells) reacts relatively slowly and incompletely with biomolecules [97]. The less cytotoxic assembly (IC50 = 0.96 μM against A2780 cells), [Ru6(p-cymene)6(tpt)2(dhbq)3]6+, on the other hand, reacts rather quickly with biomolecules, and a complete and fast disassembly could be observed with Arg, His, and Lys.


## 5.2. Tuning/Controlling the Reactivity

As shown in the previous sections, ruthenium assemblies must exhibit a certain degree of stability, in order to selectively release the encapsulated drug in the cancer cells and not in the cytosol. As discussed, the stability of metalla-assemblies is a central criterion and should be sufficient; otherwise the drug might be released in an uncontrolled manner within the human body. The stability should also not be excessive; otherwise the drug might remain encapsulated and the host-guest system might spread in an uncontrolled manner within the human body.Our results suggest a connection between the linkers used and the reactivity of the resulting metalla-assembly. To further investigate this hypothesis, studies of other arene ruthenium assemblies bearing bulkier ligands such as hexamethylbenzene instead ofp-cymene, or ruthenium assemblies with more sterically hindered linkers, are currently undertaken. The possible correlation between reactivity and cytotoxicity for these new arene ruthenium assemblies and their host-guest properties will be investigated in the near future.


## 6. Conclusion

In this review article, strategies applied to obtain tetranuclear metalla-rectangles, hexanuclear metalla-prisms, and octanuclear metalla-cubes and their host-guest properties have been extensively described. As shown along this review, some of these systems show great potential for host-guest chemistry and for biological applications. The straightforward synthetic strategy can be easily exploited and adapted to build up other supramolecular ruthenium assemblies for encapsulating water-insoluble bioactive molecules and photosensitizers. We are further extending this concept to arene-osmium and to rhodium and iridium half-sandwich complexes, to obtain metalla-assemblies with different properties, such as stability, solubility, and charge. We are also working on the synthesis of stimuli-responsive metalla-assemblies. Among external stimuli to initiate the release of guest molecules, we can in particular envisage the use of light, pH, temperature, and chemicals. In conclusion, the encapsulation of guest molecules in water-soluble arene ruthenium metalla-assemblies allows the guest molecule to reach cellular components and organelles that would be otherwise inaccessible and opens new perspectives for scientists.



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*Source: 589686-2014-07-17.xml*