# An Examination by Site-Directed Mutagenesis of Putative Key Residues in the Determination of Coenzyme Specificity in ClostridialNAD+-Dependent Glutamate Dehydrogenase

Authors: Joanna Griffin; Paul C. Engel
Journal: Enzyme Research
 (2011)

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## Abstract

Sequence and structure comparisons of various glutamate dehydrogenases (GDH) and other nicotinamide nucleotide-dependent dehydrogenases have potentially implicated certain residues in coenzyme binding and discrimination. We have mutated key residues inClostridium symbiosum NAD+-specific GDH to investigate their contribution to specificity and to enhance acceptance of NADPH. Comparisons with E. coli NADPH-dependent GDH prompted design of mutants F238S, P262S, and F238S/P262S, which were purified and assessed at pH 6.0, 7.0, and 8.0. They showed markedly increased catalytic efficiency with NADPH, especially at pH 8.0 (~170-fold for P262S and F238S/P262S with relatively small changes for NADH). A positive charge introduced through the D263K mutation also greatly increased catalytic efficiency with NADPH (over 100-fold at pH 8) and slightly decreased activity with NADH. At position 242, “P6” of the “core fingerprint,” where NAD+- and NADP+-dependent enzymes normally have Gly or Ala, respectively, clostridial GDH already has Ala. Replacement with Gly produced negligible shift in coenzyme specificity.


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## Body

## 1. Introduction

The alternative nicotinamide cofactors, NAD(H) and NADP(H), involved in many reactions catalysed by dehydrogenases and reductases, differ only in the presence or absence of a phosphate group esterified to the 2′-hydroxyl group of the AMP moiety, and the two oxidised coenzymes undergo the same 2-electron transfer to give the 1,4-dihydropyridine form. There is, however, a general metabolic separation of the roles of these chemically similar cofactors, and accordingly most dehydrogenases are specific for one or other coenzyme pair. Glutamate dehydrogenase (GDH) offers examples of both specificities, with NAD+-dependent members of the family (EC 1.4.1.2) responsible for the catabolic oxidative deamination of L-glutamate, and NADP(H)-dependent GDHs (EC 1.4.1.4) involved in biosynthetic reductive amination, incorporating ammonia into organic combination [1, 2]. There are, however, also “dual specificity” (presumably amphibolic) GDHs (EC 1.4.1.3), especially in higher animals and in Archaea, and the GDH group thus provides a particularly interesting context within which to consider both the structural basis and the evolution of coenzyme specificity, with a complete spectrum in terms of the stringency of the preference.The availability of solved structures for binary complexes of other dehydrogenases with nicotinamide cofactors and the recognition of an underlying similarity between the nucleotide binding domains of different dehydrogenases led to interpretations of the basis of coenzyme binding and discrimination [3–5] and a broad consensus regarding the main determinants of specificity. Thus, coenzyme specificity for many dehydrogenases is accepted to result from specific features of a common βαβ fold in the dinucleotide-binding domain first described by Rossmann et al. [6]. This fold is associated with an amino acid sequence “fingerprint” [7]. Wierenga et al. [8] recognised essential structural functions of several side-chains of this βαβ-fold in five NAD+-dependent dehydrogenases and highlighted the conserved sequence Gly-X-Gly-X-X-Gly. This “core fingerprint” for coenzyme binding consists of residues that form a hydrophobic core between the β-strands and the α-helix, and glycine residues that allow for a tight turn between the first β-strand and the following α-helix. In all these enzymes, the last residue in helix αA has to be a Gly, the first Gly of the consensus, because any larger residue would interfere with the adenine ribose. The α-helix is believed to provide a dipole, with the fractional positive charge on its N-terminus helping to anchor the pyrophosphate moiety, and the middle one of the three conserved glycines allows for a close approach of these two partners. Further along the sequence, an aspartate, usually the last residue of αβ, forms hydrogen bonds with the 2′-OH and 3′-OH of the adenine ribose and thus is a significant contributor to NAD+ binding and recognition. The residues of the “core fingerprint” are often labelled P1 to P6, with P7 being the conserved acidic residue [9]. In all, the sequence from P1 to P7 includes 11 amino acid residues.In NADP+-binding enzymes, by contrast, the third Gly of the consensus is often replaced by alanine [5, 7, 8]. Also, electrostatic repulsion by the acidic sidechain at P7 is regarded as one of the principal means by which NAD+-specific enzymes discriminate against NADP+, and so NADP+-dependent dehydrogenases usually have a smaller, uncharged side chain at P7 to admit the negatively charged phosphate group. Instead, a positively charged side-chain is found nearby for binding the phosphate.These generalised observations have prompted numerous attempts, with varying degrees of success, to switch the coenzyme specificity of dehydrogenases (e.g., [5, 11–16]). Some groups, attempting to switch from NAD+ to NADP+ specificity, have focussed on altering the P7 acidic residue to a smaller uncharged amino acid and/or introducing positive charge in order to stabilise the 2′-phosphate. Nishiyama et al. [13], working with NAD+-dependent malate dehydrogenase from T. flavus, were guided by crystallographic information and alignment with NADP+-dependent homologues, and made more extensive mutational changes. Their reasonably successful results emphasise the importance of creating space for the phosphate group of the larger coenzyme.Sometimes changed coenzyme activity ratios following mutagenesis merely reflect decreased affinity for the original coenzyme rather than increased activity with the target coenzyme (e.g., Huang et al. [14]). On the other hand, a study of NADP+-dependent glutathione reductase and NAD+-dependent lipoamide dehydrogenase [5, 11] represented a notable success. In E. coli glutathione reductase, P7 is neutral valine, and positive residues nearby provide a cushion for the negatively charged 2′-phosphate group of NADP+ [17–19]. The pyrophosphate bridge of the coenzyme is bound at the C-terminal end of a parallel β-sheet in the NADP(H) domain, its negative charge stabilized by the dipole at the N-terminal end of an α-helix. Conversely, NAD+-specific lipoamide dehydrogenase, with a homologous structure [20, 21] and similar chemistry, shows all the characteristic structural features noted above. By systematically addressing these features, Perham and colleagues reversed the coenzyme preference in both cases. More recently Rodríguez-Arnedo et al. [22] successfully reversed the coenzyme specificity of isocitrate dehydrogenase from Haloferax volcanii using sequence alignment of several isocitrate dehydrogenases without the help of a 3D-structure. A protein containing 5 mutations showed a complete switch of specificity from NADP+ to NAD+. Ehsani et al. [23] also achieved reversal of the coenzyme specificity of 2,3-butanediol dehydrogenase from Saccharomyces cerevisiae. Using in this case both sequence studies and the crystal structure of the homologous NADP(H)-dependent yeast alcohol dehydrogenase.The glutamate dehydrogenase family, however, displays unusual characteristics, calling into question some of the previous generalisations, and indeed it has been suggested that this family may offer more than one structural solution to the challenge of achieving a particular specificity [24]. At P7, for instance, the NAD+-dependent GDHs from Peptostreptococcus(now reclassified as Peptoniphilus)asaccharolyticus [25] and Clostridium difficile [26] have the predicted acidic sidechain (glutamate residue). However, P7 is also glutamate in dual-specific GDHs [1], and, even more remarkable, the NADPH-dependent GDHs, such as that from E. coli[27, 28], have aspartate at this position. Moreover, GDH from Clostridium symbiosum, also strongly NAD+-specific, has glycine (G261) at P7 [29] instead of a negative residue. Contemplating this variability, Baker et al. [30] proposed that an acidic residue at P7 in the NADPH-dependent GDHs might shift the pK of the 2′-phosphate, so that a protonated phosphate oxygen atom could act as a hydrogen-bond donor to the carboxyl side-chain at P7. They postulated two modes of NADPH binding, distinguished by the preference for either monobasic or dibasic 2′-phosphate. The type of residue at P7 would indicate the class into which any particular enzyme falls. It is possible that a glycine residue can occupy this position in the clostridial enzyme, since the 2′-hydroxyl of the adenine ribose is hydrogen-bonded instead to the side-chain of N290 [9].In the light of all these speculations, we have used site-directed mutagenesis to test the importance of postulated key residues in determining coenzyme specificity of the NAD+-dependent GDH of Clostridium symbiosum (CsGDH), for which there is a cloned gene [29] and high-resolution crystal structures [9, 31], including that of the enzyme-NAD+ complex.Sequence alignment of clostridial GDH with NADPH-dependent GDHs showed that most NADPH-dependent GDHs have serine residues at the positions corresponding to F238 and P262 in the clostridial enzyme. Since these residues are found in the coenzyme binding pocket (Figure1), it was postulated that the rings of F238 and P262 may limit the size of the pocket that might otherwise accommodate the extra phosphate on NADPH [9, 30]. F238 and P262 were therefore replaced with serine residues to create more space and provide stabilizing hydrogen bonds for the 2′-phosphate group.Figure 1
Coenzyme binding to clostridial glutamate dehydrogenase. The figure shows NAD+ bound to the coenzyme binding domain of glutamate dehydrogenase of Clostridium symbiosum. The image, made using PYMOL, is from the coordinates of the solved structure of the Enzyme-NAD+ complex [9] and highlights the various contact residues mutated in this study.The first residue on theβ-sheet following the βαβ fold in (CsGDH) is D263 (Figure 1). At this position, the NADPH-dependent GDHs either have serine as in E. coli   [27] and Salmonella typhimurium [32], or else positively charged lysine as in Neurospora crassa[33–35], Aspergillus nidulans [36], Saccharomyces cerevisiae [37], and Schwanniomyces occidentalis [38] (Figure 2). Lysine is also found in the dual-specific enzyme from Pyrococcus furiosus [39]. D263 of clostridial GDH was, accordingly, replaced with a lysine.Figure 2
Alignment of the relevant stretch of amino acid sequence in a number of different GDHs. The arrows indicate the three positions targeted for mutagenesis in this study and the numbers in brackets the residue numbers. The abbreviated biological species are CsClostridium symbiosum, Cd Clostridium difficile, Pa Peptostreptococcus asaccharolyticus, Hs Halobacterium salinarum, Ec Escherichia coli, St Salmonella typhimurium, ?unidentified bacterium, Ch Chlorella sorokiniana, Nc Neurospora crassa, An Aspergillus nidulans, Sc Saccharomyces cerevisiae, So Schwanniomyces occidentalis. The coenzyme specificity of the source GDH is indicated for all the monospecific enzymes, the others being dual-specific.Finally, the P6 alanine at position 242 was replaced by the glycine more usually expected in NAD+-dependent enzymes.


## 2. Experimental

### 2.1. Mutagenesis

Four single mutants, F238S, P262S, D263K, and A242G were constructed by site-directed mutagenesis using the “Quikchange” kit from Stratagene Inc. The Phe codon TTT was replaced by TCT (Ser), the Pro codon CCA with TCA (Ser), the Asp codon GAC with AAG (Lys), and the Ala codon GCA by GGA (Gly). Double-stranded plasmid DNA was extracted fromE. coliTG1 cells harbouring a ptac85 vector containing the wildtype Clostridium symbiosum gdhgene [29]. This was used as the template for the mutagenic PCR sequence, 18 cycles, each comprising 30-second denaturation at 95°C, 1-minute annealing at 55°C, and 12-minute polymerisation at 68°C with high-fidelity Pfu polymerase. The mutagenic oligonucleotides, synthesised commercially by Genosys (UK), are listed in Table 1 (in the case of the A242 position, the primers were designed also to encode the A242V substitution; although this was successfully carried out, the results were not felt to be of sufficient interest to include in this paper). The double mutant F238S/P262S was constructed by extracting the DNA encoding the F238S enzyme and mutating it further with the primer for the P262S mutation.Table 1
Sequences of oligonucleotides used in this study.
NameSequence 5′-3′Corresponding position in wildtype geneA242GfGCGCCCCATMCTACGTTACC921–940A242GrGGTAACGTAGKATGGGGCGC921–940F238SfGCATTAGCAGGTTCTGGTAACGTAGCATGGGG906–937F238SrCCCCATGCTACGTTACCAGAACCTGCTAATGC906–937P262SfGTTACACTTTCCGGATCAGACGGATACATCTACG974–1007P262SrCGTAGATGTATCCGTCTGATCCGGAAAGTGTAAC974–1007D263KfCAGTTACACTTTCCGGACCAAAGGGATACATCTACGATCCG973–1012D263KrCGGATCGTAGATGTATCCCTTTGGTCCGGAAAGTGTAACTG973–1012SeqTCCTATGGGTGGTGCCAAAG563–582In all cases, original unmutated template DNA was digested withDpnI(1 h at 37°C) and the mutated genes in the ptac-85 vector were transformed into E. coli XL1-Blue cells. Selected colonies were grown overnight and plasmids were purified using Wizard minipreps. The presence of the (mutated) gdh gene was checked by running the DNA on minielectrophoresis gels with and without digestion by BamH1 andSal1. The mutations and absence of any additional changes were confirmed by sequencing of the DNA by Cytomix Ltd.


### 2.2. Expression and Purification of Mutant Enzymes

In each case, a 1 mL overnight culture of transformants ofE. coli XL1-Blue containing the mutated gene was inoculated into 2×500 mL LB broth in 2 L flasks, supplemented with 10 μg/mL ampicillin and 0.5 mM IPTG. Bacterial cells, grown at 28°C for A242G, P262S, and the double mutant and 37°C for F238S, were harvested by centrifugation and disrupted by sonication. After recentrifugation, supernatants and pellets were run on SDS-PAGE gels to check that the overexpressed protein was soluble. In an alternative procedure to avoid formation of inclusion bodies in the cases of the double mutant and D263K mutant, 10 mL overnight culture was diluted 50X into TB phosphate medium supplemented with 100 μg/mL ampicillin, grown at 37°C to A500 of ~1, and then cooled to approximately 8°C; after 30 min, 0.1 mM IPTG was added and the cells were incubated overnight (approximately 16 hours), still at 8°C. Harvested cells were processed as above. The mutant enzymes and the recombinant wildtype clostridial GDH were all purified by dye-ligand chromatography on Remazol Red Sepharose [40].


### 2.3. Examination of Coenzyme Specificity

Kinetic parameterskcat and apparent Km were obtained by measuring initial rates of reaction for the mutant and wildtype enzymes in 0.1 M potassium phosphate (pH 6.0, 7.0, and 8.0). NAD(P)H concentrations were varied over the range 0.001–0.3 mM with fixed concentrations of 20 mM 2-oxoglutarate and 100 mM ammonium chloride. Activity at 25°C was measured either by recording the decrease in A340 with a Kontron Uvikon 941 or Cary 50 recording spectrophotometer or, for the lower coenzyme concentrations, fluorometrically (Hitachi F-4500) with excitation and emission at 340 nm, and 450 nm, respectively.


## 2.1. Mutagenesis

Four single mutants, F238S, P262S, D263K, and A242G were constructed by site-directed mutagenesis using the “Quikchange” kit from Stratagene Inc. The Phe codon TTT was replaced by TCT (Ser), the Pro codon CCA with TCA (Ser), the Asp codon GAC with AAG (Lys), and the Ala codon GCA by GGA (Gly). Double-stranded plasmid DNA was extracted fromE. coliTG1 cells harbouring a ptac85 vector containing the wildtype Clostridium symbiosum gdhgene [29]. This was used as the template for the mutagenic PCR sequence, 18 cycles, each comprising 30-second denaturation at 95°C, 1-minute annealing at 55°C, and 12-minute polymerisation at 68°C with high-fidelity Pfu polymerase. The mutagenic oligonucleotides, synthesised commercially by Genosys (UK), are listed in Table 1 (in the case of the A242 position, the primers were designed also to encode the A242V substitution; although this was successfully carried out, the results were not felt to be of sufficient interest to include in this paper). The double mutant F238S/P262S was constructed by extracting the DNA encoding the F238S enzyme and mutating it further with the primer for the P262S mutation.Table 1
Sequences of oligonucleotides used in this study.
NameSequence 5′-3′Corresponding position in wildtype geneA242GfGCGCCCCATMCTACGTTACC921–940A242GrGGTAACGTAGKATGGGGCGC921–940F238SfGCATTAGCAGGTTCTGGTAACGTAGCATGGGG906–937F238SrCCCCATGCTACGTTACCAGAACCTGCTAATGC906–937P262SfGTTACACTTTCCGGATCAGACGGATACATCTACG974–1007P262SrCGTAGATGTATCCGTCTGATCCGGAAAGTGTAAC974–1007D263KfCAGTTACACTTTCCGGACCAAAGGGATACATCTACGATCCG973–1012D263KrCGGATCGTAGATGTATCCCTTTGGTCCGGAAAGTGTAACTG973–1012SeqTCCTATGGGTGGTGCCAAAG563–582In all cases, original unmutated template DNA was digested withDpnI(1 h at 37°C) and the mutated genes in the ptac-85 vector were transformed into E. coli XL1-Blue cells. Selected colonies were grown overnight and plasmids were purified using Wizard minipreps. The presence of the (mutated) gdh gene was checked by running the DNA on minielectrophoresis gels with and without digestion by BamH1 andSal1. The mutations and absence of any additional changes were confirmed by sequencing of the DNA by Cytomix Ltd.


## 2.2. Expression and Purification of Mutant Enzymes

In each case, a 1 mL overnight culture of transformants ofE. coli XL1-Blue containing the mutated gene was inoculated into 2×500 mL LB broth in 2 L flasks, supplemented with 10 μg/mL ampicillin and 0.5 mM IPTG. Bacterial cells, grown at 28°C for A242G, P262S, and the double mutant and 37°C for F238S, were harvested by centrifugation and disrupted by sonication. After recentrifugation, supernatants and pellets were run on SDS-PAGE gels to check that the overexpressed protein was soluble. In an alternative procedure to avoid formation of inclusion bodies in the cases of the double mutant and D263K mutant, 10 mL overnight culture was diluted 50X into TB phosphate medium supplemented with 100 μg/mL ampicillin, grown at 37°C to A500 of ~1, and then cooled to approximately 8°C; after 30 min, 0.1 mM IPTG was added and the cells were incubated overnight (approximately 16 hours), still at 8°C. Harvested cells were processed as above. The mutant enzymes and the recombinant wildtype clostridial GDH were all purified by dye-ligand chromatography on Remazol Red Sepharose [40].


## 2.3. Examination of Coenzyme Specificity

Kinetic parameterskcat and apparent Km were obtained by measuring initial rates of reaction for the mutant and wildtype enzymes in 0.1 M potassium phosphate (pH 6.0, 7.0, and 8.0). NAD(P)H concentrations were varied over the range 0.001–0.3 mM with fixed concentrations of 20 mM 2-oxoglutarate and 100 mM ammonium chloride. Activity at 25°C was measured either by recording the decrease in A340 with a Kontron Uvikon 941 or Cary 50 recording spectrophotometer or, for the lower coenzyme concentrations, fluorometrically (Hitachi F-4500) with excitation and emission at 340 nm, and 450 nm, respectively.


## 3. Results

### 3.1. Overexpression and Purity of the Mutant Enzymes

The mutated genes were in the ptac-85 vector [30], which has an IPTG-inducible tac promoter to overproduce the mutant protein. 10% SDS-PAGE of the soluble and insoluble samples showed that, for F238S and P262S and A242G single mutants, the overexpressed protein was soluble at 37°C. However, in the case of the double mutant and the D263K mutant, overexpressed protein was located in the pellet in the form of insoluble inclusion bodies. Lowering the growth temperature to 28°C served to solubilise the F238S/P262S protein whilst the cold overexpression method at 8°C resulted in abundant production of soluble D263K mutant protein. After purification, each mutant protein exhibited a single prominent band on SDS-PAGE with equivalent subunit molecular mass to wildtype GDH. From 1 L overnight-grown culture, 80–100 mg pure enzyme was obtained for the wildtype, approximately 50 mg for the single mutants and 40 mg for the double mutant enzyme. Figure 3 is a representative SDS-PAGE gel showing for the D263K mutant the level of overexpression in the crude cell extract (Lane 2) and the final state of purity (Lanes 3 and 4).Figure 3
10% SDS PAGE illustrating overexpression and purification of D263K enzyme. Lane 1, molecular weight markers. Lane 2, overexpressed D263K. Lanes 3 and 4, purified D263K.


### 3.2. Kinetics

In all cases straightforward Michaelis-Menten behaviour was observed, in keeping with the previous report [40] that the 3-substrate reaction with this enzyme is not complicated by regulatory behaviour. Figure 4 shows a set of typical plots, in this case for pH 8 and for both coenzymes, NADH and NADPH, with the wildtype enzyme, the single mutants F238S and P262S and the double mutant F238S/P262S. Values of kcat, Km, and kcat/Km for the reduced coenzymes at pH 6, 7, and 8 are presented in Table 2. In all cases these are “apparent” constants, not extrapolated to saturating concentrations of the other substrates, although the fixed substrate concentrations were relatively high. With the unmutated wildtype enzyme and NADH as coenzyme there was a 12-fold increase in kcat between pH 6 and pH 8. The Km for NADH was lower at pH 7 (20 μM) than at either pH 6 (36 μM) or pH 8 (78 μM). With NADPH, however, there was a markedly different pattern, with kcat decreasing 4.4-fold from pH 6 to pH 8 and Km increasing 9-fold over the same range. As a result of these combined trends, the discrimination between the nonphosphorylated and phosphorylated cofactors is highly pH dependent. This factor, calculated as the ratio between values of kcat/Km for NADH and NADPH, is 1930 (3000/1.5) at pH 8, 203 (6500/32) at pH 7, and only 9.4 (550/59) at pH 6.Table 2
Comparison of kinetic parameters between wildtype, and F238S, P262S and F238S/P262S mutant enzymes. To determine kinetic parameters for NAD(P)H, NH4Cl, and oxoglutarate concentrations were kept constant at 100 mM and 20 mM, respectively, over a range of NAD(P)H concentrations (0.001–0.3 mM) under standard assay conditions. All experiments were repeated in triplicate, and the kinetic parameters and their standard errors (±SE) were calculated by the Wilkinson nonlinear regression method [10] with Enzpack version 3.0 (Biosoft Ltd., UK).
NADHNADPHpHkcat  (s-1)Km (mM)kcat/Km (s-1 mM)kcat  (s-1)Km (mM)kcat/Km (s-1 mM)Wildtype6.019.9±0.30.036±0.0115533.59±0.230.061±0.0158.9A242G6.011.2±1.30.034±0.0103291.43±0.010.074±0.0119.3F238S6.013.6±0.70.028±0.0044855.00±0.331.0±0.025.00P262S6.014.4±1.00.018±0.00580014.8±1.11.1±0.1613.5F238S/P262S6.013.9±1.10.048±0.01228911.2±1.52.00±0.465.75D263K6.014.6±0.60.022±0.00166320.0±1.60.068±0.010294Wildtype7.0130.2±3.10.020±0.00165002.87±0.110.090±0.01131.9A242G7.065.1±1.60.025±0.00126041.87±0.010.386±0.054.84F238S7.0139±50.040±0.003347511.1±0.210.23±0.0548.3P262S7.0160±80.047±0.103340462.5±1.60.11±0.01568F238S/P262S7.0105±40.035±0.010300083.3±3.50.33±0.02252D263K7.080±2.50.023±0.002347826.3±0.350.107±0.02246Wildtype8.0232±220.078±0.01329740.82±0.110.53±0.071.54A242G8.0177±90.085±0.0102082~0.15NDNDF238S8.0402±150.230±0.01817473.61±0.210.462±0.0877.81P262S8.0490±370.142±0.016345088.5±1.150.330±0.108268F238S/P262S8.0133±30.204±0.064665142±2.20.56±0.12221D263K8.0150±110.091±0.010164837.5±0.170.154±0.06232Lineweaver-Burk plots of initial rates for wildtype Cs GDH • and mutants F238S ■, P262S ♦, and F238S/P262S ▲ as a function of varying NADH (a) and NADPH (b and c) concentrations at pH 8.0.
(a)(b)(c)A242G showed a decreased overall catalytic efficiency for NADH at all pH values studied as compared to the wildtype enzyme. It did, however, have comparableKm values. This mutation had a severe effect on the overall catalytic efficiency with NADPH as coenzyme. At pH 6 there was a 40% reduction in kcat while Km values were comparable. At pH 7 there was a 15-fold increase in Km and at pH 8 rates were too low to measure, presumably owing to a further increase in Km (Table 2).F238S showed an unexpected increase (roughly 2-fold) inkcat for NADH at pH 8. Nevertheless, the overall catalytic efficiency of F238S with NADH was lower at all three pH values owing to an increased Km at pH 7 and 8, and the decreased value for kcat at pH 6. In the case of NADPH, kcat also increased (only 1.4-fold at pH 6, but about 4-fold at pH 7 and 8; Table 2). The improvements in kcat at pH 7 and 8 were also accompanied by opposing increases in Km, but the overall catalytic efficiency for this mutant with NADPH was nevertheless higher than that of the wildtype enzyme at pH 7 (1.5-fold) and 8 (5-fold) resulting in a modest overall change in specificity in the direction of NADPH (Table 2).Much more substantial changes in specificity were seen with the P262S mutant. With NADH the changes broadly follow the pattern of the F238S mutant, with increases inkcat of 2.1-fold at pH 8 roughly counterbalanced by increases in Km (Table 2). However, this mutation produced much larger changes with NADPH. At all three pH values tested, kcat values were greatly increased, 4-fold at pH 6, 22-fold at pH 7, and 108-fold at pH 8. At pH 6 the overall catalytic efficiency nevertheless actually decreased compared to the wildtype enzyme owing to an 18-fold increase in Km. At pH 7, however, the Km was comparable to the wildtype value, and at pH 8 it was approximately 40% lower. As a result, the overall catalytic efficiency of this mutant with NADPH was 17-fold higher at pH 7 and 172-fold higher at pH 8, as compared with the unmutated enzyme.With the F238S/P262S double mutant, (Table2) the kcat values for NADH were decreased only 1.2-fold at pH 7 and 1.7-fold at pH 8. Again, however, an increase in Km resulted in decreased overall catalytic efficiency. This mutant also had a high catalytic efficiency with NADPH as coenzyme (Table 3). The double mutant showed a greater increase in kcat over wildtype than either of the single mutants, 29-fold at pH 7 and 173-fold at pH 8. Although these increased kcat values were accompanied by further moderate increases in Km, the combined effect of these changes was a 640-fold switch in coenzyme specificity at pH 8 from an NADH : NADPH ratio of 1930 : 1 for wildtype to 3 : 1 for the double mutant. A > 4-fold decrease in efficiency for NADH was accompanied by a 144-fold increase in efficiency for NADPH, in keeping with the original intention of the mutagenesis experiments.Table 3
Summary, for each enzyme at each pH, based on data from Table2, of the extent of discrimination against NADPH and in favour of NADH. The figure, given in the third column, is calculated as the ratio of the catalytic efficiency, kcat/Km, with NADH to that with NADPH. These ratios for each mutant are then in turn compared with the figure for the wildtype enzyme to obtain the figures in the fourth column, which indicate the extent to which the mutations have reversed the preference for NADH.
EnzymepHNADH/NADPH preferenceShift in preferenceWildtype6.09.4—F238S6.0970.096xP262S6.0590.16xF238S/P262S6.0500.19xD263K6.02.34.1xWildtype7.0203—F238S7.071.92.2xP262S7.06.026.2xF238S/P262S7.011.913.2xD263K7.014.111.1xWildtype8.01930—F238S8.02248.6xP262S8.012.9150xF238S/P262S8.03.0643xD263K8.07.1272xThe D263K mutation produced remarkably little change in the kinetic parameters for NADH at the three pH values examined. However, with NADPH an entirely different pattern was seen: at all three pH values thekcat for the mutant was much higher than for wildtype GDH, and this factor increased from 5.6 at pH 6 to 9.2 at pH 7 and 46 at pH 8. As mentioned above, in the wildtype enzyme there is a strong pH dependence in the level of discrimination between NADH and NADPH. One of the outcomes of the D263K mutation was to largely remove this pH sensitivity over the range pH 6 (2.3-fold discrimination) to 8 (7.1-fold).


## 3.1. Overexpression and Purity of the Mutant Enzymes

The mutated genes were in the ptac-85 vector [30], which has an IPTG-inducible tac promoter to overproduce the mutant protein. 10% SDS-PAGE of the soluble and insoluble samples showed that, for F238S and P262S and A242G single mutants, the overexpressed protein was soluble at 37°C. However, in the case of the double mutant and the D263K mutant, overexpressed protein was located in the pellet in the form of insoluble inclusion bodies. Lowering the growth temperature to 28°C served to solubilise the F238S/P262S protein whilst the cold overexpression method at 8°C resulted in abundant production of soluble D263K mutant protein. After purification, each mutant protein exhibited a single prominent band on SDS-PAGE with equivalent subunit molecular mass to wildtype GDH. From 1 L overnight-grown culture, 80–100 mg pure enzyme was obtained for the wildtype, approximately 50 mg for the single mutants and 40 mg for the double mutant enzyme. Figure 3 is a representative SDS-PAGE gel showing for the D263K mutant the level of overexpression in the crude cell extract (Lane 2) and the final state of purity (Lanes 3 and 4).Figure 3
10% SDS PAGE illustrating overexpression and purification of D263K enzyme. Lane 1, molecular weight markers. Lane 2, overexpressed D263K. Lanes 3 and 4, purified D263K.


## 3.2. Kinetics

In all cases straightforward Michaelis-Menten behaviour was observed, in keeping with the previous report [40] that the 3-substrate reaction with this enzyme is not complicated by regulatory behaviour. Figure 4 shows a set of typical plots, in this case for pH 8 and for both coenzymes, NADH and NADPH, with the wildtype enzyme, the single mutants F238S and P262S and the double mutant F238S/P262S. Values of kcat, Km, and kcat/Km for the reduced coenzymes at pH 6, 7, and 8 are presented in Table 2. In all cases these are “apparent” constants, not extrapolated to saturating concentrations of the other substrates, although the fixed substrate concentrations were relatively high. With the unmutated wildtype enzyme and NADH as coenzyme there was a 12-fold increase in kcat between pH 6 and pH 8. The Km for NADH was lower at pH 7 (20 μM) than at either pH 6 (36 μM) or pH 8 (78 μM). With NADPH, however, there was a markedly different pattern, with kcat decreasing 4.4-fold from pH 6 to pH 8 and Km increasing 9-fold over the same range. As a result of these combined trends, the discrimination between the nonphosphorylated and phosphorylated cofactors is highly pH dependent. This factor, calculated as the ratio between values of kcat/Km for NADH and NADPH, is 1930 (3000/1.5) at pH 8, 203 (6500/32) at pH 7, and only 9.4 (550/59) at pH 6.Table 2
Comparison of kinetic parameters between wildtype, and F238S, P262S and F238S/P262S mutant enzymes. To determine kinetic parameters for NAD(P)H, NH4Cl, and oxoglutarate concentrations were kept constant at 100 mM and 20 mM, respectively, over a range of NAD(P)H concentrations (0.001–0.3 mM) under standard assay conditions. All experiments were repeated in triplicate, and the kinetic parameters and their standard errors (±SE) were calculated by the Wilkinson nonlinear regression method [10] with Enzpack version 3.0 (Biosoft Ltd., UK).
NADHNADPHpHkcat  (s-1)Km (mM)kcat/Km (s-1 mM)kcat  (s-1)Km (mM)kcat/Km (s-1 mM)Wildtype6.019.9±0.30.036±0.0115533.59±0.230.061±0.0158.9A242G6.011.2±1.30.034±0.0103291.43±0.010.074±0.0119.3F238S6.013.6±0.70.028±0.0044855.00±0.331.0±0.025.00P262S6.014.4±1.00.018±0.00580014.8±1.11.1±0.1613.5F238S/P262S6.013.9±1.10.048±0.01228911.2±1.52.00±0.465.75D263K6.014.6±0.60.022±0.00166320.0±1.60.068±0.010294Wildtype7.0130.2±3.10.020±0.00165002.87±0.110.090±0.01131.9A242G7.065.1±1.60.025±0.00126041.87±0.010.386±0.054.84F238S7.0139±50.040±0.003347511.1±0.210.23±0.0548.3P262S7.0160±80.047±0.103340462.5±1.60.11±0.01568F238S/P262S7.0105±40.035±0.010300083.3±3.50.33±0.02252D263K7.080±2.50.023±0.002347826.3±0.350.107±0.02246Wildtype8.0232±220.078±0.01329740.82±0.110.53±0.071.54A242G8.0177±90.085±0.0102082~0.15NDNDF238S8.0402±150.230±0.01817473.61±0.210.462±0.0877.81P262S8.0490±370.142±0.016345088.5±1.150.330±0.108268F238S/P262S8.0133±30.204±0.064665142±2.20.56±0.12221D263K8.0150±110.091±0.010164837.5±0.170.154±0.06232Lineweaver-Burk plots of initial rates for wildtype Cs GDH • and mutants F238S ■, P262S ♦, and F238S/P262S ▲ as a function of varying NADH (a) and NADPH (b and c) concentrations at pH 8.0.
(a)(b)(c)A242G showed a decreased overall catalytic efficiency for NADH at all pH values studied as compared to the wildtype enzyme. It did, however, have comparableKm values. This mutation had a severe effect on the overall catalytic efficiency with NADPH as coenzyme. At pH 6 there was a 40% reduction in kcat while Km values were comparable. At pH 7 there was a 15-fold increase in Km and at pH 8 rates were too low to measure, presumably owing to a further increase in Km (Table 2).F238S showed an unexpected increase (roughly 2-fold) inkcat for NADH at pH 8. Nevertheless, the overall catalytic efficiency of F238S with NADH was lower at all three pH values owing to an increased Km at pH 7 and 8, and the decreased value for kcat at pH 6. In the case of NADPH, kcat also increased (only 1.4-fold at pH 6, but about 4-fold at pH 7 and 8; Table 2). The improvements in kcat at pH 7 and 8 were also accompanied by opposing increases in Km, but the overall catalytic efficiency for this mutant with NADPH was nevertheless higher than that of the wildtype enzyme at pH 7 (1.5-fold) and 8 (5-fold) resulting in a modest overall change in specificity in the direction of NADPH (Table 2).Much more substantial changes in specificity were seen with the P262S mutant. With NADH the changes broadly follow the pattern of the F238S mutant, with increases inkcat of 2.1-fold at pH 8 roughly counterbalanced by increases in Km (Table 2). However, this mutation produced much larger changes with NADPH. At all three pH values tested, kcat values were greatly increased, 4-fold at pH 6, 22-fold at pH 7, and 108-fold at pH 8. At pH 6 the overall catalytic efficiency nevertheless actually decreased compared to the wildtype enzyme owing to an 18-fold increase in Km. At pH 7, however, the Km was comparable to the wildtype value, and at pH 8 it was approximately 40% lower. As a result, the overall catalytic efficiency of this mutant with NADPH was 17-fold higher at pH 7 and 172-fold higher at pH 8, as compared with the unmutated enzyme.With the F238S/P262S double mutant, (Table2) the kcat values for NADH were decreased only 1.2-fold at pH 7 and 1.7-fold at pH 8. Again, however, an increase in Km resulted in decreased overall catalytic efficiency. This mutant also had a high catalytic efficiency with NADPH as coenzyme (Table 3). The double mutant showed a greater increase in kcat over wildtype than either of the single mutants, 29-fold at pH 7 and 173-fold at pH 8. Although these increased kcat values were accompanied by further moderate increases in Km, the combined effect of these changes was a 640-fold switch in coenzyme specificity at pH 8 from an NADH : NADPH ratio of 1930 : 1 for wildtype to 3 : 1 for the double mutant. A > 4-fold decrease in efficiency for NADH was accompanied by a 144-fold increase in efficiency for NADPH, in keeping with the original intention of the mutagenesis experiments.Table 3
Summary, for each enzyme at each pH, based on data from Table2, of the extent of discrimination against NADPH and in favour of NADH. The figure, given in the third column, is calculated as the ratio of the catalytic efficiency, kcat/Km, with NADH to that with NADPH. These ratios for each mutant are then in turn compared with the figure for the wildtype enzyme to obtain the figures in the fourth column, which indicate the extent to which the mutations have reversed the preference for NADH.
EnzymepHNADH/NADPH preferenceShift in preferenceWildtype6.09.4—F238S6.0970.096xP262S6.0590.16xF238S/P262S6.0500.19xD263K6.02.34.1xWildtype7.0203—F238S7.071.92.2xP262S7.06.026.2xF238S/P262S7.011.913.2xD263K7.014.111.1xWildtype8.01930—F238S8.02248.6xP262S8.012.9150xF238S/P262S8.03.0643xD263K8.07.1272xThe D263K mutation produced remarkably little change in the kinetic parameters for NADH at the three pH values examined. However, with NADPH an entirely different pattern was seen: at all three pH values thekcat for the mutant was much higher than for wildtype GDH, and this factor increased from 5.6 at pH 6 to 9.2 at pH 7 and 46 at pH 8. As mentioned above, in the wildtype enzyme there is a strong pH dependence in the level of discrimination between NADH and NADPH. One of the outcomes of the D263K mutation was to largely remove this pH sensitivity over the range pH 6 (2.3-fold discrimination) to 8 (7.1-fold).


## 4. Discussion

In examining the kinetic results for the wildtype clostridial enzyme, it is interesting to note the opposite pH dependence for the two cofactors. With NADPH, increasing pH from 6 to 8 raises theKm and decreases kcat. This is in line with prediction, since over this pH range the phosphate should undergo deprotonation, increasing its local negative charge and thus making it more difficult to bind at the intended site and presumably also to achieve a catalytically productive orientation when bound. Accordingly also, the discrimination between the two cofactors is much more pronounced at pH 8 (nearly 2000-fold) than at pH 6 (9.4-fold). Kinetic parameters show that all three single mutants and the double mutant had increased overall catalytic efficiency with NADPH as predicted. However, the improvements, where seen, were in kcat values, and not in Km values as might have been anticipated. One must therefore consider the possibility that the wildtype structure allows after all a binding of NADPH that is reasonably tight but is catalytically unfavourable or inactive. The mutations then may not tighten overall binding but nevertheless might increase the fraction of bound molecules that are in a catalytically productive orientation.This suggestion is strongly supported by measurements of coenzyme dissociation constants. These measurements, made by studying each coenzyme’s ability to protect the active site –SH of Cys-320 against modification by DTNB at pH 7 [41], provided startling results. First of all, the dissociation constants of the wildtype enzyme for NADH and NADPH were virtually identical, 23 μM and 24 μM, respectively, suggesting that low activity with NADPH reflects not so much weak binding as unproductive binding. Secondly, the mutations resulted in weaker binding of both coenzymes, in some cases markedly so. Thus, for example, P262S, which shows the best kinetic improvement with NADPH at pH 7, shows a 2-fold increase in Kd for NADH (52 μM) and 16-fold increase for NADPH (375 μM). Thus, in this case, but also in the others, in purely binding terms, the mutations appear to discriminate even further against NADPH. This finding contains an important lesson for protein engineering, namely, that a substrate molecule may have more than one binding mode, and, in seeking to optimise performance, it is desirable to weaken unproductive binding as well as to strengthen the catalytic binding mode. One obvious caveat is that the binding measurements are for formation of binary complexes, and it is entirely possible that the presence of glutamate could alter the distribution between different coenzyme binding modes. It would nevertheless now be very interesting to see crystal structures for both NADH and NADPH bound to the wildtype enzyme and to one or more of these mutants. It is also important, in considering what appear at first sight to be obvious contradictions between kinetic results and measurements of dissociation constants, to recognise that we have as yet no proper analysis of overall kinetic mechanism for this GDH. Correspondingly we have no basis for assuming the relationship between the Km value for a coenzyme and the Kd for its binding to form a binary complex. If, for example, like bovine GDH [42, 43], this enzyme follows a rapid-equilibrium random-order pathway, then the Km should be equated instead with the Kd for dissociation from the ternary enzyme-glutamate-coenzyme complex. It is reasonable to expect some synergy in binding of the two substrates, and this could then account for cases where the measured Kd is substantially higher than the Km value determined here.It is also noteworthy that all the single mutants and the double mutant can still function well with NADH as coenzyme, with the overall catalytic efficiency of P262S being higher than that measured for the wildtype at pH 6 and 8. These increases in catalytic efficiency were largely due to increases inkcat, as Km values for all mutants were increased using NADH as coenzyme at all pH values measured. The intended shift in discrimination between the two coenzymes has thus been achieved not by disfavouring the activity with NADH, but almost entirely by increasing the activity with NADPH.The beneficial effects of the D263K mutation in terms of improved acceptance of NADPH relative to NADH are almost entirely due to substantial shifts inkcat values, and once again there is no simple correlation between changes in values of Kd for the binary enzyme-coenzyme complexes and the corresponding effects on Km values. Whilst there is little change in the Km values at pH 7, the Kd values for both coenzymes are increased (5-fold for NADH and 10-fold for NADPH). Aspartic acid at position 263 in the clostridial enzyme is equivalent to a serine in the E. coli enzyme and a glutamate in the Peptostreptococcus (Peptoniphilus) asaccharolyticus NAD+-dependent GDH. The importance of this position has also been examined in the latter enzyme by site-directed mutagenesis [42]. Replacement of E145 by lysine again produced a shift in specificity in the direction of NADPH, but less pronounced than for the clostridial enzyme and certainly not resulting in a good catalyst. In the case of the P.  asaccharolyticus GDH, replacing the P7 Glu by Lys resulted, much more impressively, in a genuinely dual-specificity GDH about 20-fold less active than the wildtype with NADH. Lysine, however, is not found at this position in any of the GDH sequences studied to date, regardless of coenzyme specificity, and as already noted, in clostridial GDH this position is occupied not by a negative residue but by glycine.If the “fingerprint rules” were to be applied, the A242G mutant might perhaps have been expected to use NADH at an even greater rate than the wildtype enzyme. This clearly was not the case: replacement of A242 with glycine resulted in a mutant showing considerably decreased catalytic efficiency with both NADH and NADPH. These results do, however, confirm the importance of the P6 position in coenzyme binding.Although this programme of mutagenesis has been guided by a high-resolution structure, it is important to bear in mind (a) that a binary complex structure is available only for the enzyme-NAD+ complex [9] and (b) that no structure is available for a ternary complex of clostridial GDH. It is known, however, that the binding of glutamate produces a considerable hinge movement, tending to close up the active-site cleft [31]. It seems very likely that 2-oxoglutarate may do the same. This is more likely to affect the details of the binding of the nicotinamide moiety than the adenosine, but clearly more structural information would now be helpful.Overall the targeted mutations described here altered coenzyme specificity, as predicted, in the direction of NADPH. The double mutant, in particular, behaves essentially as a dual-specific enzyme. Further mutations in this area might further encourage productive NADPH binding and, with judicious combination of mutations, it should be possible to achieve a more complete reversal of coenzyme preference. These observations serve to underline that our understanding of coenzyme specificity in dehydrogenases is as yet only partial, and there is an urgent need for more binary complex structures.



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*Source: 595793-2011-08-16.xml*