# Bioactivities of Compounds fromElephantopus scaber, an Ethnomedicinal Plant from Southwest China

Authors: Jianjun Wang; Ping Li; Baosai Li; Zhiyong Guo; Edward J. Kennelly; Chunlin Long
Journal: Evidence-Based Complementary and Alternative Medicine
 (2014)

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## Abstract

Elephantopus scaber is an ethnomedicinal plant used by the Zhuang people in Southwest China to treat headaches, colds, diarrhea, hepatitis, and bronchitis. A new δ-truxinate derivative, ethyl, methyl 3,4,3′,4′-tetrahydroxy-δ-truxinate (1), was isolated from the ethyl acetate extract of the entire plant, along with 4 known compounds. The antioxidant activity of these 5 compounds was determined by ABTS radical scavenging assay. Compound 1 was also tested for its cytotoxicity effect against HepG2 by MTT assay (IC50=60 μM), and its potential anti-inflammatory, antibiotic, and antitumor bioactivities were predicted using target fishing method software.


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## Body

## 1. Introduction

Elephantopus is a genus comprised of about 30 species worldwide, mainly distributed in South America, with only 2 speciesE. scaber andE. tomentosus found in Southwest China [1]. From 2008 to 2012, our ethnobotanical investigation in the traditional medicinal market, held during the Dragon-Boat Festival in the fifth month of the Chinese lunar calendar with a history of over 700 years, found thatElephantopus scaber L. (Asteraceae) is a common medicinal plant used by the Zhuang people in Jingxi County of Southwest China. The local Zhuang people useE. scaber commonly as a traditional herbal medicine to treat many ailments including headaches, colds, diarrhea, hepatitis, and bronchitis.To date, 30 compounds have been reported fromE. scaber, including 4 sesquiterpene lactones, 9 triterpenes, and 5 flavones. Previous bioactivity studies onE. scaber demonstrated that the extracts or compounds from this species have antibiosis, antivirus, and cytotoxicity activities [2]. The sesquiterpene lactones in particular have been explored for their anti-inflammatory and hepatoprotective activities [3], which partially proved the traditional knowledge ofE. scaber.In this paper, the isolation and structure elucidation of a new ethyl, methyl 3,4,3′,4′-tetrahydroxy-δ-truxinate (1, Figure 1) is reported, together with 4 known compounds, 5-O-caffeoylquinic acid (2) [4], chlorogenic acid methyl ester (3) [5], deoxyelephantopin (4), and isoscarbertopin (5) [6]. The radical scavenging activity of these 5 compounds was conducted using the ABTS method. The cytotoxicity effect against HpeG2 cell line of the new compound was determined by MTT assay, and the IC50 value (24.0 μg/mL) was obtained. In addition, the potential activity of1, calculated with target fishing, which used 3D structures of compounds to identify their interacting proteins by virtual screening [7], is also presented.Figure 1
The chemical structures of compounds obtained fromElephantopus scaber.


## 2. Materials and Method

### 2.1. Plant Material

The whole plant ofE. scaber was collected from the traditional medicinal market during the Dragon-Boat Festival of Jingxi County (Guangxi), Southwest China, and identified by Professor Chunlin Long. A voucher specimen was deposited in the Herbarium of Minzu University of China, numbered 201006023.


### 2.2. Extraction and Isolation

The air-dried and ground whole plant ofE. scaber (4.0 kg) was extracted with EtOH : H2O (90 : 10) at reflux for 3 × 3 h. The solvent was evaporated under reduced pressure to yield dark brown material (372.4 g). The latter was suspended in H2O (3 L) and individually partitioned with petroleum ether (3 × 3 L), Chloroform (2 × 3 L), EtOAc (3 × 3 L), andn-BuOH (3 × 3 L) to obtain petroleum ether (169.4 g), Chloroform (33.8 g), EtOAc (46.9 g), andn-BuOH (122.3 g) phase.The EtOAc phase was separated by silica gel column chromatography (CC) eluted with CHCl3 : CH3OH in order of increasing polarity to give seven fractions on the basis of TLC. Fraction 3 was subjected to MCI CC eluted with CH3OH : H2O to seven fractions A1–A7. Fraction A1 was isolated by Sephadex LH-20, ODS CC (CH3OH : H2O = 44 : 56), and Si gel CC (CHCl3 : CH3OH = 14 : 1) successively to afford compound1 (24.0 mg). Fraction A2 was purified with Sephadex LH-20 to give six subfractions. Subfraction 2 was subjected to ODS CC (CH3OH : H2O = 30 : 70) and silica gel CC (CHCl3 : CH3OH = 12 : 1) successively to give compound3(17.0 mg). Subfraction 3 was subjected to ODS CC (CH3OH : H2O = 48 : 52) and Sephadex LH-20 to afford compound2 (27.0 mg).The petroleum ether phase was separated by silica gel CC eluted with petroleum ether : EtOAc (100 : 1–0 : 100) to give ten fractions. Fraction 8 was purified by MCI CC using CH3OH : H2O (60 : 100–100 : 0) to afford four fractions B1–B4. Fraction B2 was subjected to Sephadex LH-20 and ODS CC (CH3OH : H2O = 83 : 17) to give compound4(9.0 mg). Fraction B3 was isolated by ODS CC (CH3OH : H2O = 80 : 20) and Sephadex LH-20 to give compound5(7.0 mg).


### 2.3. Antioxidant Assay

The antioxidant activity of compounds1–5 was evaluated with ABTS radical scavenging assay as described previously [8]. The IC50 was expressed as millimoles per liter (mM).


### 2.4. Cytotoxicity Assay

Compound1 was tested for cytotoxicity using a slightly modified MTT method [9]. Briefly 150 μL (10 μM, 20 μM, 30 μM, and 40 μM) of samples was added to 96-well plate containing a confluent HepG2 cell monolayer in sextuplicate; 10 μg/mL of norcantharidin (NCTD) and blank medium were used as the positive and control group, respectively. After a 72 h incubation at 37°C, 100 μL MTT solution (5 mg/mL phosphate buffered saline) was added to each well, which was further incubated for 4 h for the formation of the formazan product. After removing the medium, 150 μL DMSO was added to dissolve the formazan crystals. The optical density (OD) was measured at 550 nm with a microplate reader. The rate of inhibition was calculated by the following formula: rate of inhibition = (1 − sample OD)/control OD. The concentration causing inhibition of viable cells by 50% (IC50) was determined from a dose-response curve, which was based on triplicate measurements.


### 2.5. Virtual Screening

The potential activity of compound1was predicted by the “Target Fishing” functional model software (Discovery Studio). The target fishing process was conducted as follows. The DockScore energy function was utilized to minimize the energy of compound1conformation. Setting full minimization as minimization gave the smart conformation of compound1. Then, pharmacophore search was set to be screened and profiled. Screen and profile was set to be ligand profiler. PharmaDB pharmacophores were set to be all. Conformation generation was set to be the best. Maximum conformation was set to be 200. Energy threshold was set to be 10. Saved conformations were set to be true, and other parameters were set to be default.Top 14 candidate receptors were ranked according to the fit value (as shown in Table2), which is based on force field approximation and specifically examined the compound internal energy and the compound-receptor interaction energy, which is taken as the sum of van der Waal force and electrostatic energy [10].


### 2.6. Ethyl, Methyl 3,4,3′,4′-tetrahydroxy-δ-truxinate

Light yellow oil; [α]D −2.0°(c 0.018, MeOH); UV (in MeOH): λmax⁡ 284 and 228 nm; IR νmax⁡ ATR (cm−1): 3436, 2924, 2854, 1736, and 1600–1450; HRESIMS (m/z): 403.1286 [M+H]+; 1H NMR (300 MHz, CD3OD): δH 6.73 (4H, d-like), 6.62 (1H, t,J = 6.0, 3.0 Hz), 6.59 (1H, t,J = 6.0, 3.0 Hz), 4.19 (2H, q,J = 7.1 Hz), 3.73 (3H, s), 3.43 (1H, d-like,J = 2.9 Hz), 3.40 (1H, d-like,J = 2.5 Hz), 3.30 (1H, d-like,J = 3.1 Hz), 3.27 (1H, d-like,J = 3.5 Hz), and 1.26 (3H, t,J = 14.2, 7.1 Hz). 13C NMR (75 MHz, CD3OD): δC 173.5, 173.0, 145.0, 144.1, 132.8, 117.6, 115.0, 113.5, 60.7, 51.2, 50.2, 50.0, 46.2, 45.8, and 13.2.


## 2.1. Plant Material

The whole plant ofE. scaber was collected from the traditional medicinal market during the Dragon-Boat Festival of Jingxi County (Guangxi), Southwest China, and identified by Professor Chunlin Long. A voucher specimen was deposited in the Herbarium of Minzu University of China, numbered 201006023.


## 2.2. Extraction and Isolation

The air-dried and ground whole plant ofE. scaber (4.0 kg) was extracted with EtOH : H2O (90 : 10) at reflux for 3 × 3 h. The solvent was evaporated under reduced pressure to yield dark brown material (372.4 g). The latter was suspended in H2O (3 L) and individually partitioned with petroleum ether (3 × 3 L), Chloroform (2 × 3 L), EtOAc (3 × 3 L), andn-BuOH (3 × 3 L) to obtain petroleum ether (169.4 g), Chloroform (33.8 g), EtOAc (46.9 g), andn-BuOH (122.3 g) phase.The EtOAc phase was separated by silica gel column chromatography (CC) eluted with CHCl3 : CH3OH in order of increasing polarity to give seven fractions on the basis of TLC. Fraction 3 was subjected to MCI CC eluted with CH3OH : H2O to seven fractions A1–A7. Fraction A1 was isolated by Sephadex LH-20, ODS CC (CH3OH : H2O = 44 : 56), and Si gel CC (CHCl3 : CH3OH = 14 : 1) successively to afford compound1 (24.0 mg). Fraction A2 was purified with Sephadex LH-20 to give six subfractions. Subfraction 2 was subjected to ODS CC (CH3OH : H2O = 30 : 70) and silica gel CC (CHCl3 : CH3OH = 12 : 1) successively to give compound3(17.0 mg). Subfraction 3 was subjected to ODS CC (CH3OH : H2O = 48 : 52) and Sephadex LH-20 to afford compound2 (27.0 mg).The petroleum ether phase was separated by silica gel CC eluted with petroleum ether : EtOAc (100 : 1–0 : 100) to give ten fractions. Fraction 8 was purified by MCI CC using CH3OH : H2O (60 : 100–100 : 0) to afford four fractions B1–B4. Fraction B2 was subjected to Sephadex LH-20 and ODS CC (CH3OH : H2O = 83 : 17) to give compound4(9.0 mg). Fraction B3 was isolated by ODS CC (CH3OH : H2O = 80 : 20) and Sephadex LH-20 to give compound5(7.0 mg).


## 2.3. Antioxidant Assay

The antioxidant activity of compounds1–5 was evaluated with ABTS radical scavenging assay as described previously [8]. The IC50 was expressed as millimoles per liter (mM).


## 2.4. Cytotoxicity Assay

Compound1 was tested for cytotoxicity using a slightly modified MTT method [9]. Briefly 150 μL (10 μM, 20 μM, 30 μM, and 40 μM) of samples was added to 96-well plate containing a confluent HepG2 cell monolayer in sextuplicate; 10 μg/mL of norcantharidin (NCTD) and blank medium were used as the positive and control group, respectively. After a 72 h incubation at 37°C, 100 μL MTT solution (5 mg/mL phosphate buffered saline) was added to each well, which was further incubated for 4 h for the formation of the formazan product. After removing the medium, 150 μL DMSO was added to dissolve the formazan crystals. The optical density (OD) was measured at 550 nm with a microplate reader. The rate of inhibition was calculated by the following formula: rate of inhibition = (1 − sample OD)/control OD. The concentration causing inhibition of viable cells by 50% (IC50) was determined from a dose-response curve, which was based on triplicate measurements.


## 2.5. Virtual Screening

The potential activity of compound1was predicted by the “Target Fishing” functional model software (Discovery Studio). The target fishing process was conducted as follows. The DockScore energy function was utilized to minimize the energy of compound1conformation. Setting full minimization as minimization gave the smart conformation of compound1. Then, pharmacophore search was set to be screened and profiled. Screen and profile was set to be ligand profiler. PharmaDB pharmacophores were set to be all. Conformation generation was set to be the best. Maximum conformation was set to be 200. Energy threshold was set to be 10. Saved conformations were set to be true, and other parameters were set to be default.Top 14 candidate receptors were ranked according to the fit value (as shown in Table2), which is based on force field approximation and specifically examined the compound internal energy and the compound-receptor interaction energy, which is taken as the sum of van der Waal force and electrostatic energy [10].


## 2.6. Ethyl, Methyl 3,4,3′,4′-tetrahydroxy-δ-truxinate

Light yellow oil; [α]D −2.0°(c 0.018, MeOH); UV (in MeOH): λmax⁡ 284 and 228 nm; IR νmax⁡ ATR (cm−1): 3436, 2924, 2854, 1736, and 1600–1450; HRESIMS (m/z): 403.1286 [M+H]+; 1H NMR (300 MHz, CD3OD): δH 6.73 (4H, d-like), 6.62 (1H, t,J = 6.0, 3.0 Hz), 6.59 (1H, t,J = 6.0, 3.0 Hz), 4.19 (2H, q,J = 7.1 Hz), 3.73 (3H, s), 3.43 (1H, d-like,J = 2.9 Hz), 3.40 (1H, d-like,J = 2.5 Hz), 3.30 (1H, d-like,J = 3.1 Hz), 3.27 (1H, d-like,J = 3.5 Hz), and 1.26 (3H, t,J = 14.2, 7.1 Hz). 13C NMR (75 MHz, CD3OD): δC 173.5, 173.0, 145.0, 144.1, 132.8, 117.6, 115.0, 113.5, 60.7, 51.2, 50.2, 50.0, 46.2, 45.8, and 13.2.


## 3. Results

Compound1(28.0 mg) was separated from the ethyl acetate extract ofE. scaber whole plant as a light yellow oil. The molecular formula C21H22O8 was determined by the molecular ion observed atm/z 403.1359 [M+H]+ in the LC-TOF-MS (positive mode), which requires 11 degrees of unsaturation. The IR spectrum presented bands in the 1600–1450 cm−1, 1736 cm−1, 2854 cm−1, 2924 cm−1, and 3436 cm−1 region, which corresponded to aromatic, ester, methyl or methylene, and phenolic hydroxyl groups, respectively. The structure of compound1 was further elucidated by examination of its 1D 13C (75 MHz), DEPT (90° and 135°), and 1H (300 MHz) NMR spectra and HMQC, HMBC, COSY, and NOESY spectra in MeOH-d4. Only 13 carbon resonance signals and the other two carbon signals overlapped by the solvent carbon signals were found in 13C NMR and DEPT spectra, respectively, which suggested that there are many identical parts in this molecule. Further analysis of the 13C NMR spectrum of compound1suggested each signal of δC 145.0, 144.1, 132.8, 117.6, 115.0, and 113.5 is comprised of two overlapping carbon signals [δC 145.0 (C-3 and C-3′), 144.1 (C-4 and C-4′), 132.8 (C-1 and C-1′), 117.6 (C-6 and C-6′), 115.0 (C-5 and C-5′), and 113.5 (C-2 and C-2′)]; the other signals were assigned to two carbonyl carbon atoms [δ 173.5 (C-9) and 173.0 (C-9′)], one methoxy group (δC 60.7, C-10), one submethoxy (δC 51.2, C-10′), methyl (δC 13.2, C-11′), and four methine carbons [δC 50.2 (C-7′), 50.0 (C-7), 46.2 (C-8′), and 45.8 (C-8)]. From the number of unsaturations and carbons, these four methane carbons were deduced to be cyclobutane ring. From the analysis of 1H NMR spectrum of compound1, two phenylpropanoid units were presented at δH 6.73 (4H, d-like, H-2, H-5, H-2′, H-5′), δH 6.62 (1H, t,J = 6.0, 3.0 Hz, H-6/H-6′), and 6.59 (1H, t,J = 6.0, 3.0 Hz, H-6′/H-6). Four methane carbons of the cyclobutane ring were observed at δH 3.43 (1H, d-like,J = 2.9 Hz, H-7), 3.40 (1H, d-like,J= 2.5 Hz, H-7′), 3.30 (1H, d-like,J = 3.1 Hz, H-8′), and 3.27 (1H, d-like,J = 3.5 Hz, H-8), and the relative configuration of the cyclobutane ring was determined by comparing the chemical shift of compound1 with reported 1H NMR data of otherδ-truxinate derivatives [11]. Other signals of 1H NMR spectra were assigned to submethoxy [δH 4.19 (2H, q,J = 7.1 Hz, H-10′)], methoxy [δH 3.73 (3H, s, H-10)], and methyl [δH 1.26 (3H, t,J = 14.2, 7.1 Hz, H-11′)]. Meanwhile, the HMBC spectrum of compound1 presented the correlations from H-10 to C-9, H-8 to C-9 and C-8′, H-7 to C-2 and C-6, H-11′ to C-10′, from C-10′ to H-9′ and H-11′, from C-8′ to C-9′, and from C-7′ to H-2′ and H-6′, respectively (Figure 2). Consequently, the structure of compound1 was deduced to be ethyl, methyl 3,4,3′,4′-tetrahydroxy-δ-truxinate, which was further confirmed by HMQC, COSY, and NOESY spectra. This paper reports a newδ-truxinate derivative inElephantopus genus for the first time. Compounds2–5 were identified, respectively, as 5-O-caffeoylquinic acid (2), chlorogenic acid methyl ester (3), deoxyelephantopin (4), and isoscarbertopin (5) by comparing their NMR and MS data with reported literature values.Figure 2
Selected HMBC (proton to carbon) correlations of compound1.The antioxidant activity of 5 compounds isolated fromE. scaber was evaluated by the ABTS radical scavenging assay, and the results are presented as IC50 in Table 1. The most active radical scavengers were the new compound ethyl, methyl 3,4,3′,4′-tetrahydroxy-δ-truxinate (IC50 = 0.44±0.039 mM). The other 2 quinic acid derivatives 5-O-caffeoylquinic acid and chlorogenic acid methyl ester also showed radical scavenging potential (IC50 = 0.96±0.096 and 0.89±0.140 mM, resp.), while the antioxidant activity of the other 2 sesquiterpene lactone compounds deoxyelephantopin and isoscabertopin was not detected. Comparing the structures of these 5 compounds, the different antioxidant activities were attributed to the existence of phenolic hydroxyl groups in compounds, which were supported by the previous reports [12].Table 1
The radical scavenging activity of 5 compounds fromElephantopus scaber.
Compound
Name
IC50 (mM)a
1
Ethyl, methyl 3,4,3′,4′-tetrahydroxy-δ-truxinate
0.44
±
0.039
2
5-O-caffeoylquinic acid
0.96
±
0.096
3
Chlorogenic acid methyl ester
0.89
±
0.140
4
Deoxyelephantopin
NR
5
Isoscarbertopin
NR
6
b
Trolox
1.33
±
0.187
aThe inhibition was recorded at 10 min of reaction (ABTS method) and IC50 value was measured using PROBIT model: PROBIT (p) = intercept + BX (covariates X are transformed using the base 10.000 logarithm). Each value corresponds to the mean and standard deviation of duplicates at five concentrations.
bPositive control group.
NR: No reaction at the conditions discribed.Table 2
The potential bioactivity screening results of compound1.
Pharmacophore
Name of pharmacophore
Type
Fit value
Biological function(s)
Reference
2zb8-01-s
Prostaglandin reductase 2
Protein
4.05271
Inflammation
[14]
3kjs-01
Dihydrofolate reductase-thymidylate synthase
Protein
3.9615
Malarial parasites, anticancer, and inflammation
[15–17]
2uue-01
Cell division protein kinase 2
Protein
3.60758
Cell division
[18, 19]
2w4i-01-s
Glutamate racemase
Protein
3.55547
Antibiotics
[20–24]
3k6l-01
Peptide deformylase
Protein
3.51102
Antibiotic
[25, 26]
3md7-01
beta-lactamase-like
Protein
3.41887
Antibiotic
[27, 28]
2ovy-01
Phosphodiesterase 10A
Protein
3.41834
Schizophrenia andnervous system
[29–31]
3ac8-01
Protooncogene tyrosine-protein kinase LCK
Protein
3.39142
Antitumor
[32, 33]
3f7z-01
G17 glycogen synthase kinase-3-beta
Protein
3.30076
Antitumor and neurodegenerative disease
[34]
3cgy-01
Virulence sensor histidine kinase phoQ
Protein
3.28695
Antibiotic
[35, 36]
2qe5-01
RNA-directed RNA polymerase
Protein
3.28198
Antivirus
[37, 38]
1dvx-01
Transthyretin
Protein
3.27421
Antitumor and obesity
[39, 40]
2brc-01
ATP-dependent molecular chaperone HSP90
Protein
3.26648
Antitumor and antivirus
[41, 42]
1c1b-01-s
HIV-1 reverse transcriptase (A-chain)
Protein
3.25549
Anti-HIV
[43–45]Compound1was also tested forin vitro cytotoxicity against HepG2 cell line with norcantharidin (NCTD, 60 μM) as positive control at 72 h incubation (Figure 3). Compound1exhibited a dose-response inhibition curve from 27% growth inhibition at 10 μg/mL to 81% at 40 μg/mL, demonstrating that it has significant and dose-dependent inhibition on the growth of HepG2 (IC50 = 60 μM). Further work will be conducted on the mechanism by which compound1 induces apoptosis.Figure 3
Growth inhibition of compound1 on HepG2 cell lines, where each value represents mean ± standard deviation of 6 replicates (n=6), as compared to the positive control norcantharidin (60 μM) (P*<0.001 for comparison of control cell with cells treated with compound1 and NCTD); 72 h for the incubation period of cells after being treated with compound1 and NCTD.With the rapid development of computer-aided drug design (CADD), virtual screening technique has been used more and more widely in drug design and bioactivity screening of compounds [13]. The potential bioactivities of compound1have been predicted by the target fishing method which was based on the Discovery Studio software and Protein Date Bank (PDB) including over twelve thousand 3D macromolecular structure data determined experimentally by X-ray crystallography and NMR. The top 14 biological molecular targets ranked as the fit value (FV) are reported (Table 2).Theoretically, FV > 3 means this target should be explored experimentally. The strongest activity of compound1 was predicted to be anti-inflammatory (FV = 4.05271) and anti-AIDS (FV = 3.25549), respectively (Table 2). Further experiments on the biological functions of1should be directed towards its potential anti-inflammatory, antibiotic, antivirus, and anticancer activities.


## 4. Discussion and Conclusion

With more and more present modern drugs discovered from traditional medical knowledge, the traditional knowledge is getting more extensive attention, which also led to the development of important drugs such as reserpine (a treatment for hypertension) podophyllotoxin (the base of an important anticancer drug), and vinblastine (used in the treatment of certain cancers) [46].Previous studies showed that pulmonary oxidant stress can cause some disease conditions, such as acute lung injury, radiation injury, COPD (chronic obstructive pulmonary disease), and inflammation [47]. Meanwhile, previous clinical and experimental studies described that antioxidant supplementation including flavonoids and vitamins may inverse the oxidant-mediated cough depression by modulating the inflammatory process in lung disease [48, 49]. Interestingly, our work using ABTS assay demonstrated that compounds1–3 showed strong antioxidant activity, especially compound1(IC50 = 0.44±0.039 mM). Moreover,E. scaber was also reported as the source of a number of sesquiterpene lactones, such as compounds4 and5, which have shown significant contribution to the anti-inflammatory activity of plants [50]. Some of the sesquiterpenes from the genusElephantopus have demonstrated significant anti-inflammatory as well as hepatoprotective activities and are being considered as drug lead compounds [3]. Based on the above analysis, we hypothesize that Zhuang people use this plant to treat headaches, bronchitis, and hepatitis,due to its anti-inflammatory and antioxidant effects.According to thein vitro cytotoxicity assay with NCTD (60 μM) as control group and activity virtual screening, compound1exhibited good (IC50 = 60 μM) and dose-response inhibition on HepG2 cell line and potential anti-inflammatory, antibiotic, antivirus, and anticancer activities, which indicated that the further research ofE. scaber could be focused on anticancer and anti-inflammatory activity. The present work further developed the usage of this traditional medicine plant.



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*Source: 569594-2014-05-19.xml*