# Preparation and Optimization of 10-Hydroxycamptothecin Nanocolloidal Particles Using Antisolvent Method Combined with High Pressure Homogenization

Authors: Bolin Lian; Yong Li; Xiuhua Zhao; Yuangang Zu; Ying Wang; Yin Zhang; Yuanyuan Li
Journal: Journal of Chemistry
 (2017)

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## Abstract

The aim of this study was to prepare 10-hydroxycamptothecin nanocolloidal particles (HCPTNPs) to increase the solubility of drugs, reduce the toxicity, improve the stability of the drug, and so forth. HCPTNPs was prepared by antisolvent precipitation (AP) method combined with high pressure homogenization (HPH), followed by lyophilization. The main parameters during antisolvent process including volume ratio of dimethyl sulfoxide (DMSO) and H2O and dripping speed were optimized and their effects on mean particle size (MPS) and yield of HCPT primary particles were investigated. In the high pressure homogeneous procedure, types of surfactants, amount of surfactants, and homogenization pressure (HP) were optimized and their influences on MPS, zeta potential (ZP), and morphology were analyzed. The optimum conditions of HCPTNPs were as follows: 0.2 mg/mL HCPT aqueous suspension, 1% of ASS, 1000 bar of HP, and 20 passes. Finally, the HCPTNPs via lyophilization using glucose as lyoprotectant under optimum conditions had an MPS of 179.6 nm and a ZP of 28.79 ± 1.97 mV. The short-term stability of HCPTNPs indicated that the MPS changed in a small range.


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## Body

## 1. Introduction

10-Hydroxycamptothecin (HCPT), one analogue of camptothecin, has been shown to have a strong antitumor activity against a wide range of experimental tumors including gastric carcinoma, hepatoma, leukemia, and head and neck tumors [1]. Moreover, it has been proven to be more potent and less toxic in experimental animals and in human clinical evaluation compared to CPT. However, its poor solubility in water and in other physiologically acceptable organic solvents hinders its clinical application [2–6]. To improve the solubility and stability of HCPT, many new drug delivery systems including microspheres, liposomes, and micelles system were developed [7].Nanoparticles have been successfully used to solve the poor solubility of HCPT [6, 8–14]. Up to now, there are mainly two kinds of method [15]. One is to nucleate and assemble drug molecular into nanoparticles, which consists of two major approaches, preparation of micropowder [16] and surfactants [17]. Another is to prepare large drug particles into nanoparticles using high pressure homogenization [18]. In addition, supercritical fluid antisolvent (SAS) has also been widely studied [19, 20].Though the nanoparticles prepared by SAS have a small and uniform particle size, defects of small batch and high production cost limit its practical use. However, the particles prepared by antisolvent precipitation method at atmospheric pressure have a wide particle size distribution. It is rapid and easy to operate and industrialize compared to SAS. In particular, it is suitable to prepare primary particles. High pressure homogenization (HPH) is an effective tool to prepare refined particles and emulsion products. The HPH technique has merits of reducing particle size of the preparation and obtaining a narrow and uniform particle size distribution over the other methods such as ultrasonication, milling, and high speed shear.In our study, antisolvent precipitation (AP) at atmospheric pressure combined HPH was utilized to prepare HCPTNPs. The AP process parameters including volume ratio of dimethyl sulfoxide (DMSO) and H2O and dripping speed were optimized and their effects on mean particle size (MPS) and yield of HCPT were discussed. HCPT primary particles obtained under optimum conditions were further prepared into HCPTNPs using HPH. The optimum HPH procedure including types of surfactants, amount of surfactants, and homogenization pressure (HP) was determined. Finally, the MPS, zeta potential (ZP), and short-term stability of HCPTNPs obtained under optimum HPH conditions via lyophilization were researched.


## 2. Materials and Methods

### 2.1. Materials

HCPT (>98%) was provided by Hisun Pharmaceutical (Taizhou, China). Dimethyl sulfoxide (DMSO), phosphatidylcholine, sodium tauroglycocholate, poloxamer 188, sodium sulfate, gelatin, and glucose were obtained from Sigma-Aldrich (St. Louis, MO, USA).


### 2.2. Preparation of HCPT Primary Particles

The HCPT primary particles were prepared by antisolvent precipitation. Firstly, 5 mg/mL of raw HCPT was dissolved in DMSO. Then the drug solution was totally injected into aqueous solution with different volume at different flow rates by injection pump (JZB-1800D, Chien-Yuan Medical Technology Co., Ltd., Changsha, China). The aqueous solution was being stirred for 10 min at a certain speed until the drug solution dripped off. Then HCPT suspension was centrifuged at 10,000 rpm for 15 minutes and washed for three times to eliminate organic solvent DMSO by water. The final precipitate was just HCPT nanoparticles. Each experiment was repeated three times.


### 2.3. Preparation of HCPTNPs

Fifty milliliters of 0.2 mg/mL HCPT primary particles aqueous suspension that added certain quality of surfactants was processed using HPH. Then HCPTNPs were obtained under the conditions of a certain HP and passes.


### 2.4. Optimization of HCPTNPs

Due to the hydrophilicity of surfactants, the solubility of HCPT can be enhanced to some degree through the combination of different kinds of surfactants. Therefore, six kinds of surfactants were chosen to be selected, namely, lecithin, sodium taurocholate, poloxamer 188, anhydrous sodium sulfate (ASS), sodium dodecyl sulfate (SDS), and gelatin. The adding ratio of different kinds of surfactants, HP, and passes were optimized to obtain the optimum preparation conditions of HCPTNPs. The adding ratios of surfactants were according to volume ratio of preparation system. The volume ratios were separately 1% (0.5 mg) and 5% (2.5 mg). The experiment design was shown in Table1.Table 1
Experimental design of preparation of HCPTNPs.
HP
Lecithin
Sodium taurocholate
ASS
Poloxamer 188
Gelatin
SDS
600 bar
0.5 mg
0.5 mg
0.5 mg
0.5 mg
0.5 mg
0.5 mg
2.5 mg
2.5 mg
2.5 mg
2.5 mg
2.5 mg
2.5 mg
800 bar
0.5 mg
0.5 mg
0.5 mg
0.5 mg
0.5 mg
0.5 mg
2.5 mg
2.5 mg
2.5 mg
2.5 mg
2.5 mg
2.5 mg
1000 bar
0.5 mg
0.5 mg
0.5 mg
0.5 mg
0.5 mg
0.5 mg
2.5 mg
2.5 mg
2.5 mg
2.5 mg
2.5 mg
2.5 mg


### 2.5. Particle Size and ZP Analysis

Dynamic light scattering (DLS) (ZetaPALS, Brookhaven Instruments) with a He-Ne laser (632.8 nm, 35 mW) as light source was employed to detect MPS and ZP of samples. The sample was prepared by dilution using pure water and every measurement was repeated three times.


### 2.6. Morphology Observations

The morphology of particles was ascertained by SEM (Quanta 200, FEI). Samples were installed on aluminum stubs by means of double-sided carbon tape and sputter-coated with gold under argon atmosphere.


### 2.7. Lyophilization

Based on the optimization HPH, the HCPTNPs aqueous suspension was added to glucose which was three times the amount of HCPTNPs. After mixing process, samples were continuously taken out and joined in 8 mL glass vials. The HCPTNPs were lyophilized by a Gamma 2–20 apparatus (Christ, Germany) to obtain dry powder with good physicochemical stability. Freezing temperature was set −40°C for 2 h to process the samples. Subsequently, lyophilization was underway at −40°C for 48 h, followed by a secondary drying phase of 12 h at 20°C.


### 2.8. Short-Term Stability in Purified Water of HCPTNPs

The redispersibility study of HCPTNPs via lyophilization in purified water was detected by DLS equipment at 36.7°C. The MPS and ZP of freeze-dried HCPTNPs were analyzed at predetermined time intervals (0, 1, 2, 4, 6, and 8 h). Moreover, HCPT primary particles, HCPTNPs, and freeze-dried HCPTNPs which had the same concentration of HCPT were separately dispersed in a single vial with 4 mL purified water. The dispersion appearances of three samples were captured by camera.


## 2.1. Materials

HCPT (>98%) was provided by Hisun Pharmaceutical (Taizhou, China). Dimethyl sulfoxide (DMSO), phosphatidylcholine, sodium tauroglycocholate, poloxamer 188, sodium sulfate, gelatin, and glucose were obtained from Sigma-Aldrich (St. Louis, MO, USA).


## 2.2. Preparation of HCPT Primary Particles

The HCPT primary particles were prepared by antisolvent precipitation. Firstly, 5 mg/mL of raw HCPT was dissolved in DMSO. Then the drug solution was totally injected into aqueous solution with different volume at different flow rates by injection pump (JZB-1800D, Chien-Yuan Medical Technology Co., Ltd., Changsha, China). The aqueous solution was being stirred for 10 min at a certain speed until the drug solution dripped off. Then HCPT suspension was centrifuged at 10,000 rpm for 15 minutes and washed for three times to eliminate organic solvent DMSO by water. The final precipitate was just HCPT nanoparticles. Each experiment was repeated three times.


## 2.3. Preparation of HCPTNPs

Fifty milliliters of 0.2 mg/mL HCPT primary particles aqueous suspension that added certain quality of surfactants was processed using HPH. Then HCPTNPs were obtained under the conditions of a certain HP and passes.


## 2.4. Optimization of HCPTNPs

Due to the hydrophilicity of surfactants, the solubility of HCPT can be enhanced to some degree through the combination of different kinds of surfactants. Therefore, six kinds of surfactants were chosen to be selected, namely, lecithin, sodium taurocholate, poloxamer 188, anhydrous sodium sulfate (ASS), sodium dodecyl sulfate (SDS), and gelatin. The adding ratio of different kinds of surfactants, HP, and passes were optimized to obtain the optimum preparation conditions of HCPTNPs. The adding ratios of surfactants were according to volume ratio of preparation system. The volume ratios were separately 1% (0.5 mg) and 5% (2.5 mg). The experiment design was shown in Table1.Table 1
Experimental design of preparation of HCPTNPs.
HP
Lecithin
Sodium taurocholate
ASS
Poloxamer 188
Gelatin
SDS
600 bar
0.5 mg
0.5 mg
0.5 mg
0.5 mg
0.5 mg
0.5 mg
2.5 mg
2.5 mg
2.5 mg
2.5 mg
2.5 mg
2.5 mg
800 bar
0.5 mg
0.5 mg
0.5 mg
0.5 mg
0.5 mg
0.5 mg
2.5 mg
2.5 mg
2.5 mg
2.5 mg
2.5 mg
2.5 mg
1000 bar
0.5 mg
0.5 mg
0.5 mg
0.5 mg
0.5 mg
0.5 mg
2.5 mg
2.5 mg
2.5 mg
2.5 mg
2.5 mg
2.5 mg


## 2.5. Particle Size and ZP Analysis

Dynamic light scattering (DLS) (ZetaPALS, Brookhaven Instruments) with a He-Ne laser (632.8 nm, 35 mW) as light source was employed to detect MPS and ZP of samples. The sample was prepared by dilution using pure water and every measurement was repeated three times.


## 2.6. Morphology Observations

The morphology of particles was ascertained by SEM (Quanta 200, FEI). Samples were installed on aluminum stubs by means of double-sided carbon tape and sputter-coated with gold under argon atmosphere.


## 2.7. Lyophilization

Based on the optimization HPH, the HCPTNPs aqueous suspension was added to glucose which was three times the amount of HCPTNPs. After mixing process, samples were continuously taken out and joined in 8 mL glass vials. The HCPTNPs were lyophilized by a Gamma 2–20 apparatus (Christ, Germany) to obtain dry powder with good physicochemical stability. Freezing temperature was set −40°C for 2 h to process the samples. Subsequently, lyophilization was underway at −40°C for 48 h, followed by a secondary drying phase of 12 h at 20°C.


## 2.8. Short-Term Stability in Purified Water of HCPTNPs

The redispersibility study of HCPTNPs via lyophilization in purified water was detected by DLS equipment at 36.7°C. The MPS and ZP of freeze-dried HCPTNPs were analyzed at predetermined time intervals (0, 1, 2, 4, 6, and 8 h). Moreover, HCPT primary particles, HCPTNPs, and freeze-dried HCPTNPs which had the same concentration of HCPT were separately dispersed in a single vial with 4 mL purified water. The dispersion appearances of three samples were captured by camera.


## 3. Results and Discussion

### 3.1. Experiment Analysis of HCPT Primary Particles

There are many factors that affect the preparation of HCPT using antisolvent method. In this experiment, volume ratio of DMSO and H2O and dripping speed were optimized through orthogonal experiments. The particle size along with yield of HCPT primary particles were chosen to be the response values. As shown in Table 2, the MPS and yield of HCPT primary particles changed irregularly with volume ratio of HCPT-DMSO and H2O and dripping speed. MPS in most of trials were larger than 1 μm. The yield of HCPT primary particles also plays an important role in the process cost. The yield and MPS were synthetically considered to obtain the optimized conditions, namely, 5 mg/mL of HCPT concentration, 1 : 1 of the volume ratio of DMSO and H2O, 4 mL/min of dripping speed, and 10 min of stirring time at 500 rpm. The obtained suspension was centrifuged at 10,000 rpm for 15 min. According to the optimum conditions of antisolvent method, ultrasonication was utilized to accomplish the antisolvent method. When dripping was finished, ultrasonication was going on for 10 min. The MPS and ZP of HCPT primary particles were 746 nm and −23.73 mV, separately. The particle size of drugs has an important effect in the distribution in vivo. The HCPT with large particle size has low water solubility, resulting in difficulty to shuttle across cell membranes and playing a pharmacodynamic role. So the particle size has the key role, depending on the distribution and utilization in vivo.Table 2
The factors of orthogonal and experimental result.
Volume ratio of DMSO and H2O
Dripping speed (mL/min)
MPS (nm)
Yield (%)
1 : 1
1
1284.8
82.8
2
991.6
82.6
3
1123.4
80.8
4
896.4
84.6
5
1077
83.0
1 : 3
1
1243.1
72.2
2
1005.6
77.2
3
1082.8
74.4
4
1044.1
77.0
5
1175.2
73.0
1 : 5
1
1275.7
72.4
2
820.6
76.6
3
961.9
73.8
4
1079.5
76.2
5
1011.9
71.2


### 3.2. Surface Morphology of HCPT Primary Particles

The morphology of raw HCPT and HCPT primary particles was observed by scanning electron microscopy (SEM). In Figure1(a), raw HCPT was in irregular lump crystal form with a width of 1–30 μm range. However, HCPT primary particles showed sphere morphology and uniform size. Moreover, there were not visible drug crystals.Figure 1
SEM images of (a) raw HCPT and (b) HCPT primary particles.
(a)
(b)


### 3.3. Optimization of HCPTNPs Using HPH

#### 3.3.1. Effects of Surfactants on MPS and ZP of HCPTNPs

Firstly, 0.2 mg/mL HCPT aqueous suspension was added to lecithin according to the 5% of mass ratio of HCPT powder and 5% of volume ratio of preparation system, respectively. Ultrasonication was used to make lecithin totally dissolve. HP of 600 bar, 800 bar, and 1000 bar were chosen in the homogenization process. Twenty passes were conducted under each HP. The MPS and ZP of HCPTNPs were detected under three different pressures. The results were shown in Table3. Seen from Table 3, the MPS of HCPTNPs increased with the increasing of lecithin addition. The ZP of HCPTNPs also varied with the increasing of lecithin addition. Seen from Figure 2, homogeneous degree between HCPT and lecithin was improved with the increase of HPH and passes, which caused that the MPS of HCPTNPs with 0.5 mg lecithin maintained 230–240 nm. Meanwhile, the MPS of HCPTNPs with 2.5 mg lecithin maintained 260–270 nm. The MPS of HCPTNPs was smaller, when addition of lecithin was 5% of mass ratio of HCPT powder and HP was 1000 bar. However, seen from SEM images, HCPTNPs with lecithin unevenly distributed and presented adhesion phenomenon in irregular shape.Table 3
Preparation of HCPTNPs with lecithin by HPH.
Pressure (bar)
Mass (mg)
MPS (nm)
ZP (mV)
600
0.5
85.2
11.91
±
0.92
2.5
143.1
-
24.00
±
1.27
800
0.5
233.6
-
31.35
±
0.44
2.5
272.2
-
41.73
±
1.94
1000
0.5
258.3
-
33.23
±
2.01
2.5
261.7
-
27.32
±
1.43Figure 2
SEM images of HCPTNPs using lecithin as surfactants under different HP and amount conditions: (a) 0.5 mg, 600 bar; (b) 0.5 mg, 800 bar; (c) 0.5 mg, 1000 bar; (d) 2.5 mg, 600 bar; (e) 2.5 mg, 800 bar; (f) 2.5 mg, 1000 bar.
(a)
(b)
(c)
(d)
(e)
(f)The preparation of HCPTNPs using sodium taurocholate as surfactants was referred to Section  2.3. The results were shown in Table4. From Figure 3, MPS got larger when the sodium taurocholate was added more under the same HP condition. So the amount of surfactants had an effect on MPS of HCPTNPs. When the addition of sodium taurocholate was 0.5 mg, MPS of HCPTNPs got larger and was distributed in 270 nm–340 nm with the increase of HP. Meanwhile, when the addition of sodium taurocholate was 2.5 mg, MPS of HCPTNPs was about 300 nm with the increase of HP. Seen from SEM images, the HCPTNPs dispersed well, had a smooth surface, and had a large particle size. Adhesion happened to HCPTNPs with the amount rising of sodium taurocholate.Table 4
Preparation of HCPTNPs with sodium taurocholate by HPH.
Pressure (bar)
Mass (mg)
MPS (nm)
ZP (mV)
600
0.5
269.0
-
23.12
±
1.05
2.5
329.9
-
26.22
±
1.08
800
0.5
333.6
-
31.92
±
0.18
2.5
398.6
-
22.37
±
1.44
1000
0.5
341.8
-
25.06
±
0.98
2.5
289.2
-
30.9
±
0.53Figure 3
SEM images of HCPTNPs using sodium taurocholate as surfactants under different HP and amount conditions: (a) 0.5 mg, 600 bar; (b) 0.5 mg, 800 bar; (c) 0.5 mg, 1000 bar; (d) 2.5 mg, 600 bar; (e) 2.5 mg, 800 bar; (f) 2.5 mg, 1000 bar.
(a)
(b)
(c)
(d)
(e)
(f)The experiment result using poloxamer 188 as surfactants was shown in Table5. As seen in Table 5, the MPS of HCPTNPs decreased and varied within 150–270 nm with the increase of HP when 0.5 mg poloxamer 188 was added. However, the MPS of HCPTNPs had a tendency to become large when 2.5 mg poloxamer 188 was added. So the amount of surfactant could make an influence on MPS of HCPT nanocolloidal particles. The amount of poloxamer 188 was added more; the MPS of HCPTNPs was larger. Seen from Figure 4, HCPTNPs have defects of conglutination, nonuniform particle size distribution.Table 5
Preparation of HCPTNPs with poloxamer 188 by HPH.
Pressure (bar)
Mass (mg)
MPS (nm)
ZP (mV)
600
0.5
272.6
-
21.02
±
1.82
2.5
181.9
-
28.11
±
1.25
800
0.5
214.1
-
27.42
±
1.53
2.5
147.3
-
14.79
±
1.36
1000
0.5
144.3
-
18.57
±
1.33
2.5
273.6
-
26.22
±
1.90Figure 4
SEM images of HCPTNPs using poloxamer 188 as surfactants under different HP and amount conditions: (a) 0.5 mg, 600 bar; (b) 0.5 mg, 800 bar; (c) 0.5 mg, 1000 bar; (d) 2.5 mg, 600 bar; (e) 2.5 mg, 800 bar; (f) 2.5 mg, 1000 bar.
(a)
(b)
(c)
(d)
(e)
(f)As seen in Table6, the MPS of HCPTNPs increased with the increasing of lecithin addition. The ZP of HCPTNPs also varied with the increasing of lecithin addition. The MPS of HCPTNPs decreased and varied within 236–168 nm with the increase of HP when 0.5 mg ASS was added. However, the MPS of HCPTNPs had an inapparent tendency to become small and varied within 450–234 nm when 2.5 mg ASS was added. Figure 5 indicated that HCPTNPs had a uniform particle size distribution, no visible particles, and spherical and smooth particles.Table 6
Preparation of HCPTNPs with ASS by HPH.
Pressure (bar)
Mass (mg)
MPS (nm)
ZP (mV)
600
0.5
229.5
-
32.44
±
1.47
2.5
147.5
-
34.39
±
1.18
800
0.5
154.9
-
37.57
±
0.98
2.5
224.6
-
25.03
±
1.86
1000
0.5
168.7
-
35.14
±
0.69
2.5
250.6
-
26.58
±
1.73Figure 5
SEM images of HCPTNPs using ASS as surfactants under different HP and amount conditions: (a) 0.5 mg, 600 bar; (b) 0.5 mg, 800 bar; (c) 0.5 mg, 1000 bar; (d) 2.5 mg, 600 bar; (e) 2.5 mg, 800 bar; (f) 2.5 mg, 1000 bar.
(a)
(b)
(c)
(d)
(e)
(f)Table7 indicated the amount of SDS was added more; the MPS of HCPTNPs was larger. The MPS of HCPTNPs decreased and varied within 230–169 nm with the increase of HP and passes when 0.5 mg SDS was added. Compared with 0.5 mg SDS, the MPS of HCPTNPs added to 2.5 mg SDS was larger varying within 150–250 nm. Figure 6 showed that HCPTNPs had a nonuniform particle size distribution and phenomenon of agglomeration.Table 7
Preparation of HCPTNPs with SDS by HPH.
Pressure (bar)
Mass (mg)
MPS (nm)
ZP (mV)
600
0.5
229.5
-
32.44
±
1.47
2.5
147.5
-
34.39
±
1.18
800
0.5
154.9
-
37.57
±
0.98
2.5
224.6
-
25.03
±
1.86
1000
0.5
168.7
-
35.14
±
0.69
2.5
250.6
-
26.58
±
1.73Figure 6
SEM images of HCPTNPs using SDS as surfactants under different HP and amount conditions: (a) 0.5 mg, 600 bar; (b) 0.5 mg, 800 bar; (c) 0.5 mg, 1000 bar; (d) 2.5 mg, 600 bar; (e) 2.5 mg, 800 bar; (f) 2.5 mg, 1000 bar.
(a)
(b)
(c)
(d)
(e)
(f)As shown in Table8, when the amount of gelatin was kept the same, HP was larger and passes were more, and the MPS of HCPTNPs was smaller. The MPS of HCPTNPs added to 2.5 mg gelatin was smaller in comparison with the MPS of HCPTNPs added to 0.5 mg gelatin. The MPS varied within 190–75 nm. The SEM images of Figure 7 indicated that HCPTNPs using gelatin as surfactants showed adhesion and had an uneven particle size distribution.Table 8
Preparation of HCPTNPs with gelatin by HPH.
Pressure (bar)
Mass (mg)
MPS (nm)
ZP (mV)
600
0.5
433.5
-
22.51
±
1.16
2.5
191.5
-
12.42
±
0.49
800
0.5
153.8
-
32.35
±
0.56
2.5
169.1
-
36.46
±
1.25
1000
0.5
163.3
-
31.55
±
1.16
2.5
74.9
-
23.04
±
1.04Figure 7
SEM images of HCPTNPs using gelatin as surfactants under different HP and amount conditions: (a) 0.5 mg, 600 bar; (b) 0.5 mg, 800 bar; (c) 0.5 mg, 1000 bar; (d) 2.5 mg, 600 bar; (e) 2.5 mg, 800 bar; (f) 2.5 mg, 1000 bar.
(a)
(b)
(c)
(d)
(e)
(f)


#### 3.3.2. Optimum HPH Conditions of HCPTNPs

MPS, ZP, HP, adding amount of surfactants, and morphology of HCPTNPs were all taken into account; ASS was chosen to be the best surfactant. The optimum conditions were as follows: 0.2 mg/mL HCPT aqueous suspension, 1% of ASS, 1000 bar of HP, and 20 passes. Under the optimum conditions, the MPS and ZP of HCPTNPs were 168.6 nm and −25.18 mV, respectively.


### 3.4. Short-Term Stability in Purified Water of Freeze-Dried HCPTNPs

As seen in Table9, MPS and ZP of freeze-dried HCPTNPs had minor change, which showed that freeze-dried HCPTNPs were physically and chemically stable for up to 8 h at room temperature. The precept of short-term stability study was derived from the evaluation of internationally marketable Abraxane from FDA (US Food and Drug Administration). Seen from Figure 8, the disperse states of HCPTNPs via HPH and freeze-dried HCPTNPs in water were both opalescent and showed no significant difference. Meanwhile, due to large particle size of HCPT primary particles, the water dispersant seemed turbid. In particular, some drug particles appeared upon the surface of the water.Table 9
Redispersibility of freeze-dried HCPTNPs in purified water.
Time (h)
MPS (nm)
ZP (mV)
0
179.6
-
28.79
±
1.97
1
193.3
-
27.99
±
2.21
2
221.3
-
29.98
±
0.74
4
235.4
-
23.70
±
2.08
6
247.1
-
27.87
±
1.37
8
245.8
-
30.02
±
0.67Figure 8
Dispersion appearances in water of (a) HCPT primary particles, (b) HCPT nanocolloidal particles, and (c) freeze-dried HCPT nanocolloidal particles.
(a)
(b)
(c)


## 3.1. Experiment Analysis of HCPT Primary Particles

There are many factors that affect the preparation of HCPT using antisolvent method. In this experiment, volume ratio of DMSO and H2O and dripping speed were optimized through orthogonal experiments. The particle size along with yield of HCPT primary particles were chosen to be the response values. As shown in Table 2, the MPS and yield of HCPT primary particles changed irregularly with volume ratio of HCPT-DMSO and H2O and dripping speed. MPS in most of trials were larger than 1 μm. The yield of HCPT primary particles also plays an important role in the process cost. The yield and MPS were synthetically considered to obtain the optimized conditions, namely, 5 mg/mL of HCPT concentration, 1 : 1 of the volume ratio of DMSO and H2O, 4 mL/min of dripping speed, and 10 min of stirring time at 500 rpm. The obtained suspension was centrifuged at 10,000 rpm for 15 min. According to the optimum conditions of antisolvent method, ultrasonication was utilized to accomplish the antisolvent method. When dripping was finished, ultrasonication was going on for 10 min. The MPS and ZP of HCPT primary particles were 746 nm and −23.73 mV, separately. The particle size of drugs has an important effect in the distribution in vivo. The HCPT with large particle size has low water solubility, resulting in difficulty to shuttle across cell membranes and playing a pharmacodynamic role. So the particle size has the key role, depending on the distribution and utilization in vivo.Table 2
The factors of orthogonal and experimental result.
Volume ratio of DMSO and H2O
Dripping speed (mL/min)
MPS (nm)
Yield (%)
1 : 1
1
1284.8
82.8
2
991.6
82.6
3
1123.4
80.8
4
896.4
84.6
5
1077
83.0
1 : 3
1
1243.1
72.2
2
1005.6
77.2
3
1082.8
74.4
4
1044.1
77.0
5
1175.2
73.0
1 : 5
1
1275.7
72.4
2
820.6
76.6
3
961.9
73.8
4
1079.5
76.2
5
1011.9
71.2


## 3.2. Surface Morphology of HCPT Primary Particles

The morphology of raw HCPT and HCPT primary particles was observed by scanning electron microscopy (SEM). In Figure1(a), raw HCPT was in irregular lump crystal form with a width of 1–30 μm range. However, HCPT primary particles showed sphere morphology and uniform size. Moreover, there were not visible drug crystals.Figure 1
SEM images of (a) raw HCPT and (b) HCPT primary particles.
(a)
(b)


## 3.3. Optimization of HCPTNPs Using HPH

### 3.3.1. Effects of Surfactants on MPS and ZP of HCPTNPs

Firstly, 0.2 mg/mL HCPT aqueous suspension was added to lecithin according to the 5% of mass ratio of HCPT powder and 5% of volume ratio of preparation system, respectively. Ultrasonication was used to make lecithin totally dissolve. HP of 600 bar, 800 bar, and 1000 bar were chosen in the homogenization process. Twenty passes were conducted under each HP. The MPS and ZP of HCPTNPs were detected under three different pressures. The results were shown in Table3. Seen from Table 3, the MPS of HCPTNPs increased with the increasing of lecithin addition. The ZP of HCPTNPs also varied with the increasing of lecithin addition. Seen from Figure 2, homogeneous degree between HCPT and lecithin was improved with the increase of HPH and passes, which caused that the MPS of HCPTNPs with 0.5 mg lecithin maintained 230–240 nm. Meanwhile, the MPS of HCPTNPs with 2.5 mg lecithin maintained 260–270 nm. The MPS of HCPTNPs was smaller, when addition of lecithin was 5% of mass ratio of HCPT powder and HP was 1000 bar. However, seen from SEM images, HCPTNPs with lecithin unevenly distributed and presented adhesion phenomenon in irregular shape.Table 3
Preparation of HCPTNPs with lecithin by HPH.
Pressure (bar)
Mass (mg)
MPS (nm)
ZP (mV)
600
0.5
85.2
11.91
±
0.92
2.5
143.1
-
24.00
±
1.27
800
0.5
233.6
-
31.35
±
0.44
2.5
272.2
-
41.73
±
1.94
1000
0.5
258.3
-
33.23
±
2.01
2.5
261.7
-
27.32
±
1.43Figure 2
SEM images of HCPTNPs using lecithin as surfactants under different HP and amount conditions: (a) 0.5 mg, 600 bar; (b) 0.5 mg, 800 bar; (c) 0.5 mg, 1000 bar; (d) 2.5 mg, 600 bar; (e) 2.5 mg, 800 bar; (f) 2.5 mg, 1000 bar.
(a)
(b)
(c)
(d)
(e)
(f)The preparation of HCPTNPs using sodium taurocholate as surfactants was referred to Section  2.3. The results were shown in Table4. From Figure 3, MPS got larger when the sodium taurocholate was added more under the same HP condition. So the amount of surfactants had an effect on MPS of HCPTNPs. When the addition of sodium taurocholate was 0.5 mg, MPS of HCPTNPs got larger and was distributed in 270 nm–340 nm with the increase of HP. Meanwhile, when the addition of sodium taurocholate was 2.5 mg, MPS of HCPTNPs was about 300 nm with the increase of HP. Seen from SEM images, the HCPTNPs dispersed well, had a smooth surface, and had a large particle size. Adhesion happened to HCPTNPs with the amount rising of sodium taurocholate.Table 4
Preparation of HCPTNPs with sodium taurocholate by HPH.
Pressure (bar)
Mass (mg)
MPS (nm)
ZP (mV)
600
0.5
269.0
-
23.12
±
1.05
2.5
329.9
-
26.22
±
1.08
800
0.5
333.6
-
31.92
±
0.18
2.5
398.6
-
22.37
±
1.44
1000
0.5
341.8
-
25.06
±
0.98
2.5
289.2
-
30.9
±
0.53Figure 3
SEM images of HCPTNPs using sodium taurocholate as surfactants under different HP and amount conditions: (a) 0.5 mg, 600 bar; (b) 0.5 mg, 800 bar; (c) 0.5 mg, 1000 bar; (d) 2.5 mg, 600 bar; (e) 2.5 mg, 800 bar; (f) 2.5 mg, 1000 bar.
(a)
(b)
(c)
(d)
(e)
(f)The experiment result using poloxamer 188 as surfactants was shown in Table5. As seen in Table 5, the MPS of HCPTNPs decreased and varied within 150–270 nm with the increase of HP when 0.5 mg poloxamer 188 was added. However, the MPS of HCPTNPs had a tendency to become large when 2.5 mg poloxamer 188 was added. So the amount of surfactant could make an influence on MPS of HCPT nanocolloidal particles. The amount of poloxamer 188 was added more; the MPS of HCPTNPs was larger. Seen from Figure 4, HCPTNPs have defects of conglutination, nonuniform particle size distribution.Table 5
Preparation of HCPTNPs with poloxamer 188 by HPH.
Pressure (bar)
Mass (mg)
MPS (nm)
ZP (mV)
600
0.5
272.6
-
21.02
±
1.82
2.5
181.9
-
28.11
±
1.25
800
0.5
214.1
-
27.42
±
1.53
2.5
147.3
-
14.79
±
1.36
1000
0.5
144.3
-
18.57
±
1.33
2.5
273.6
-
26.22
±
1.90Figure 4
SEM images of HCPTNPs using poloxamer 188 as surfactants under different HP and amount conditions: (a) 0.5 mg, 600 bar; (b) 0.5 mg, 800 bar; (c) 0.5 mg, 1000 bar; (d) 2.5 mg, 600 bar; (e) 2.5 mg, 800 bar; (f) 2.5 mg, 1000 bar.
(a)
(b)
(c)
(d)
(e)
(f)As seen in Table6, the MPS of HCPTNPs increased with the increasing of lecithin addition. The ZP of HCPTNPs also varied with the increasing of lecithin addition. The MPS of HCPTNPs decreased and varied within 236–168 nm with the increase of HP when 0.5 mg ASS was added. However, the MPS of HCPTNPs had an inapparent tendency to become small and varied within 450–234 nm when 2.5 mg ASS was added. Figure 5 indicated that HCPTNPs had a uniform particle size distribution, no visible particles, and spherical and smooth particles.Table 6
Preparation of HCPTNPs with ASS by HPH.
Pressure (bar)
Mass (mg)
MPS (nm)
ZP (mV)
600
0.5
229.5
-
32.44
±
1.47
2.5
147.5
-
34.39
±
1.18
800
0.5
154.9
-
37.57
±
0.98
2.5
224.6
-
25.03
±
1.86
1000
0.5
168.7
-
35.14
±
0.69
2.5
250.6
-
26.58
±
1.73Figure 5
SEM images of HCPTNPs using ASS as surfactants under different HP and amount conditions: (a) 0.5 mg, 600 bar; (b) 0.5 mg, 800 bar; (c) 0.5 mg, 1000 bar; (d) 2.5 mg, 600 bar; (e) 2.5 mg, 800 bar; (f) 2.5 mg, 1000 bar.
(a)
(b)
(c)
(d)
(e)
(f)Table7 indicated the amount of SDS was added more; the MPS of HCPTNPs was larger. The MPS of HCPTNPs decreased and varied within 230–169 nm with the increase of HP and passes when 0.5 mg SDS was added. Compared with 0.5 mg SDS, the MPS of HCPTNPs added to 2.5 mg SDS was larger varying within 150–250 nm. Figure 6 showed that HCPTNPs had a nonuniform particle size distribution and phenomenon of agglomeration.Table 7
Preparation of HCPTNPs with SDS by HPH.
Pressure (bar)
Mass (mg)
MPS (nm)
ZP (mV)
600
0.5
229.5
-
32.44
±
1.47
2.5
147.5
-
34.39
±
1.18
800
0.5
154.9
-
37.57
±
0.98
2.5
224.6
-
25.03
±
1.86
1000
0.5
168.7
-
35.14
±
0.69
2.5
250.6
-
26.58
±
1.73Figure 6
SEM images of HCPTNPs using SDS as surfactants under different HP and amount conditions: (a) 0.5 mg, 600 bar; (b) 0.5 mg, 800 bar; (c) 0.5 mg, 1000 bar; (d) 2.5 mg, 600 bar; (e) 2.5 mg, 800 bar; (f) 2.5 mg, 1000 bar.
(a)
(b)
(c)
(d)
(e)
(f)As shown in Table8, when the amount of gelatin was kept the same, HP was larger and passes were more, and the MPS of HCPTNPs was smaller. The MPS of HCPTNPs added to 2.5 mg gelatin was smaller in comparison with the MPS of HCPTNPs added to 0.5 mg gelatin. The MPS varied within 190–75 nm. The SEM images of Figure 7 indicated that HCPTNPs using gelatin as surfactants showed adhesion and had an uneven particle size distribution.Table 8
Preparation of HCPTNPs with gelatin by HPH.
Pressure (bar)
Mass (mg)
MPS (nm)
ZP (mV)
600
0.5
433.5
-
22.51
±
1.16
2.5
191.5
-
12.42
±
0.49
800
0.5
153.8
-
32.35
±
0.56
2.5
169.1
-
36.46
±
1.25
1000
0.5
163.3
-
31.55
±
1.16
2.5
74.9
-
23.04
±
1.04Figure 7
SEM images of HCPTNPs using gelatin as surfactants under different HP and amount conditions: (a) 0.5 mg, 600 bar; (b) 0.5 mg, 800 bar; (c) 0.5 mg, 1000 bar; (d) 2.5 mg, 600 bar; (e) 2.5 mg, 800 bar; (f) 2.5 mg, 1000 bar.
(a)
(b)
(c)
(d)
(e)
(f)


### 3.3.2. Optimum HPH Conditions of HCPTNPs

MPS, ZP, HP, adding amount of surfactants, and morphology of HCPTNPs were all taken into account; ASS was chosen to be the best surfactant. The optimum conditions were as follows: 0.2 mg/mL HCPT aqueous suspension, 1% of ASS, 1000 bar of HP, and 20 passes. Under the optimum conditions, the MPS and ZP of HCPTNPs were 168.6 nm and −25.18 mV, respectively.


## 3.3.1. Effects of Surfactants on MPS and ZP of HCPTNPs

Firstly, 0.2 mg/mL HCPT aqueous suspension was added to lecithin according to the 5% of mass ratio of HCPT powder and 5% of volume ratio of preparation system, respectively. Ultrasonication was used to make lecithin totally dissolve. HP of 600 bar, 800 bar, and 1000 bar were chosen in the homogenization process. Twenty passes were conducted under each HP. The MPS and ZP of HCPTNPs were detected under three different pressures. The results were shown in Table3. Seen from Table 3, the MPS of HCPTNPs increased with the increasing of lecithin addition. The ZP of HCPTNPs also varied with the increasing of lecithin addition. Seen from Figure 2, homogeneous degree between HCPT and lecithin was improved with the increase of HPH and passes, which caused that the MPS of HCPTNPs with 0.5 mg lecithin maintained 230–240 nm. Meanwhile, the MPS of HCPTNPs with 2.5 mg lecithin maintained 260–270 nm. The MPS of HCPTNPs was smaller, when addition of lecithin was 5% of mass ratio of HCPT powder and HP was 1000 bar. However, seen from SEM images, HCPTNPs with lecithin unevenly distributed and presented adhesion phenomenon in irregular shape.Table 3
Preparation of HCPTNPs with lecithin by HPH.
Pressure (bar)
Mass (mg)
MPS (nm)
ZP (mV)
600
0.5
85.2
11.91
±
0.92
2.5
143.1
-
24.00
±
1.27
800
0.5
233.6
-
31.35
±
0.44
2.5
272.2
-
41.73
±
1.94
1000
0.5
258.3
-
33.23
±
2.01
2.5
261.7
-
27.32
±
1.43Figure 2
SEM images of HCPTNPs using lecithin as surfactants under different HP and amount conditions: (a) 0.5 mg, 600 bar; (b) 0.5 mg, 800 bar; (c) 0.5 mg, 1000 bar; (d) 2.5 mg, 600 bar; (e) 2.5 mg, 800 bar; (f) 2.5 mg, 1000 bar.
(a)
(b)
(c)
(d)
(e)
(f)The preparation of HCPTNPs using sodium taurocholate as surfactants was referred to Section  2.3. The results were shown in Table4. From Figure 3, MPS got larger when the sodium taurocholate was added more under the same HP condition. So the amount of surfactants had an effect on MPS of HCPTNPs. When the addition of sodium taurocholate was 0.5 mg, MPS of HCPTNPs got larger and was distributed in 270 nm–340 nm with the increase of HP. Meanwhile, when the addition of sodium taurocholate was 2.5 mg, MPS of HCPTNPs was about 300 nm with the increase of HP. Seen from SEM images, the HCPTNPs dispersed well, had a smooth surface, and had a large particle size. Adhesion happened to HCPTNPs with the amount rising of sodium taurocholate.Table 4
Preparation of HCPTNPs with sodium taurocholate by HPH.
Pressure (bar)
Mass (mg)
MPS (nm)
ZP (mV)
600
0.5
269.0
-
23.12
±
1.05
2.5
329.9
-
26.22
±
1.08
800
0.5
333.6
-
31.92
±
0.18
2.5
398.6
-
22.37
±
1.44
1000
0.5
341.8
-
25.06
±
0.98
2.5
289.2
-
30.9
±
0.53Figure 3
SEM images of HCPTNPs using sodium taurocholate as surfactants under different HP and amount conditions: (a) 0.5 mg, 600 bar; (b) 0.5 mg, 800 bar; (c) 0.5 mg, 1000 bar; (d) 2.5 mg, 600 bar; (e) 2.5 mg, 800 bar; (f) 2.5 mg, 1000 bar.
(a)
(b)
(c)
(d)
(e)
(f)The experiment result using poloxamer 188 as surfactants was shown in Table5. As seen in Table 5, the MPS of HCPTNPs decreased and varied within 150–270 nm with the increase of HP when 0.5 mg poloxamer 188 was added. However, the MPS of HCPTNPs had a tendency to become large when 2.5 mg poloxamer 188 was added. So the amount of surfactant could make an influence on MPS of HCPT nanocolloidal particles. The amount of poloxamer 188 was added more; the MPS of HCPTNPs was larger. Seen from Figure 4, HCPTNPs have defects of conglutination, nonuniform particle size distribution.Table 5
Preparation of HCPTNPs with poloxamer 188 by HPH.
Pressure (bar)
Mass (mg)
MPS (nm)
ZP (mV)
600
0.5
272.6
-
21.02
±
1.82
2.5
181.9
-
28.11
±
1.25
800
0.5
214.1
-
27.42
±
1.53
2.5
147.3
-
14.79
±
1.36
1000
0.5
144.3
-
18.57
±
1.33
2.5
273.6
-
26.22
±
1.90Figure 4
SEM images of HCPTNPs using poloxamer 188 as surfactants under different HP and amount conditions: (a) 0.5 mg, 600 bar; (b) 0.5 mg, 800 bar; (c) 0.5 mg, 1000 bar; (d) 2.5 mg, 600 bar; (e) 2.5 mg, 800 bar; (f) 2.5 mg, 1000 bar.
(a)
(b)
(c)
(d)
(e)
(f)As seen in Table6, the MPS of HCPTNPs increased with the increasing of lecithin addition. The ZP of HCPTNPs also varied with the increasing of lecithin addition. The MPS of HCPTNPs decreased and varied within 236–168 nm with the increase of HP when 0.5 mg ASS was added. However, the MPS of HCPTNPs had an inapparent tendency to become small and varied within 450–234 nm when 2.5 mg ASS was added. Figure 5 indicated that HCPTNPs had a uniform particle size distribution, no visible particles, and spherical and smooth particles.Table 6
Preparation of HCPTNPs with ASS by HPH.
Pressure (bar)
Mass (mg)
MPS (nm)
ZP (mV)
600
0.5
229.5
-
32.44
±
1.47
2.5
147.5
-
34.39
±
1.18
800
0.5
154.9
-
37.57
±
0.98
2.5
224.6
-
25.03
±
1.86
1000
0.5
168.7
-
35.14
±
0.69
2.5
250.6
-
26.58
±
1.73Figure 5
SEM images of HCPTNPs using ASS as surfactants under different HP and amount conditions: (a) 0.5 mg, 600 bar; (b) 0.5 mg, 800 bar; (c) 0.5 mg, 1000 bar; (d) 2.5 mg, 600 bar; (e) 2.5 mg, 800 bar; (f) 2.5 mg, 1000 bar.
(a)
(b)
(c)
(d)
(e)
(f)Table7 indicated the amount of SDS was added more; the MPS of HCPTNPs was larger. The MPS of HCPTNPs decreased and varied within 230–169 nm with the increase of HP and passes when 0.5 mg SDS was added. Compared with 0.5 mg SDS, the MPS of HCPTNPs added to 2.5 mg SDS was larger varying within 150–250 nm. Figure 6 showed that HCPTNPs had a nonuniform particle size distribution and phenomenon of agglomeration.Table 7
Preparation of HCPTNPs with SDS by HPH.
Pressure (bar)
Mass (mg)
MPS (nm)
ZP (mV)
600
0.5
229.5
-
32.44
±
1.47
2.5
147.5
-
34.39
±
1.18
800
0.5
154.9
-
37.57
±
0.98
2.5
224.6
-
25.03
±
1.86
1000
0.5
168.7
-
35.14
±
0.69
2.5
250.6
-
26.58
±
1.73Figure 6
SEM images of HCPTNPs using SDS as surfactants under different HP and amount conditions: (a) 0.5 mg, 600 bar; (b) 0.5 mg, 800 bar; (c) 0.5 mg, 1000 bar; (d) 2.5 mg, 600 bar; (e) 2.5 mg, 800 bar; (f) 2.5 mg, 1000 bar.
(a)
(b)
(c)
(d)
(e)
(f)As shown in Table8, when the amount of gelatin was kept the same, HP was larger and passes were more, and the MPS of HCPTNPs was smaller. The MPS of HCPTNPs added to 2.5 mg gelatin was smaller in comparison with the MPS of HCPTNPs added to 0.5 mg gelatin. The MPS varied within 190–75 nm. The SEM images of Figure 7 indicated that HCPTNPs using gelatin as surfactants showed adhesion and had an uneven particle size distribution.Table 8
Preparation of HCPTNPs with gelatin by HPH.
Pressure (bar)
Mass (mg)
MPS (nm)
ZP (mV)
600
0.5
433.5
-
22.51
±
1.16
2.5
191.5
-
12.42
±
0.49
800
0.5
153.8
-
32.35
±
0.56
2.5
169.1
-
36.46
±
1.25
1000
0.5
163.3
-
31.55
±
1.16
2.5
74.9
-
23.04
±
1.04Figure 7
SEM images of HCPTNPs using gelatin as surfactants under different HP and amount conditions: (a) 0.5 mg, 600 bar; (b) 0.5 mg, 800 bar; (c) 0.5 mg, 1000 bar; (d) 2.5 mg, 600 bar; (e) 2.5 mg, 800 bar; (f) 2.5 mg, 1000 bar.
(a)
(b)
(c)
(d)
(e)
(f)


## 3.3.2. Optimum HPH Conditions of HCPTNPs

MPS, ZP, HP, adding amount of surfactants, and morphology of HCPTNPs were all taken into account; ASS was chosen to be the best surfactant. The optimum conditions were as follows: 0.2 mg/mL HCPT aqueous suspension, 1% of ASS, 1000 bar of HP, and 20 passes. Under the optimum conditions, the MPS and ZP of HCPTNPs were 168.6 nm and −25.18 mV, respectively.


## 3.4. Short-Term Stability in Purified Water of Freeze-Dried HCPTNPs

As seen in Table9, MPS and ZP of freeze-dried HCPTNPs had minor change, which showed that freeze-dried HCPTNPs were physically and chemically stable for up to 8 h at room temperature. The precept of short-term stability study was derived from the evaluation of internationally marketable Abraxane from FDA (US Food and Drug Administration). Seen from Figure 8, the disperse states of HCPTNPs via HPH and freeze-dried HCPTNPs in water were both opalescent and showed no significant difference. Meanwhile, due to large particle size of HCPT primary particles, the water dispersant seemed turbid. In particular, some drug particles appeared upon the surface of the water.Table 9
Redispersibility of freeze-dried HCPTNPs in purified water.
Time (h)
MPS (nm)
ZP (mV)
0
179.6
-
28.79
±
1.97
1
193.3
-
27.99
±
2.21
2
221.3
-
29.98
±
0.74
4
235.4
-
23.70
±
2.08
6
247.1
-
27.87
±
1.37
8
245.8
-
30.02
±
0.67Figure 8
Dispersion appearances in water of (a) HCPT primary particles, (b) HCPT nanocolloidal particles, and (c) freeze-dried HCPT nanocolloidal particles.
(a)
(b)
(c)


## 4. Conclusions

It had been shown that antisolvent method combined with HPH could be used to prepare HCPT nanocolloidal particles, which was physically and chemically stable in the short run. In this study, preparation technology of HCPTNPs was optimized. Characters of HCPTNPs were determined, which included MPS, ZP, redispersibility in purified water, and SEM. The results indicated that freeze-dried HCPTNPs had a uniform particle size and good stability in the short run, which demonstrated a promising prospect of the new formulation of HCPT. However, long-term stability study and in vivo research of HCPTNPs should now be proceeded to provide references for therapeutic use.



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*Source: 5752090-2017-05-24.xml*