# Ethnopharmacological Survey on Medicinal Plants Used for Cosmetic Treatments in Traditional and Ayurveda Systems of Medicine in Sri Lanka

Authors: Dehel Gamage Nadeeshani Dilhara Gamage; Rathnayaka Mudiyanselage Dharmadasa; Don Chandana Abeysinghe; Rathnayaka Gamlathge Saman Wijesekara; Gamika A. Prathapasinghe; Takao Someya
Journal: Evidence-Based Complementary and Alternative Medicine
 (2021)

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## Abstract

Medicinal plants have been used for therapeutic and beauty applications in Sri Lanka with documented history of over 2,500 years. This inherited knowledge, which has been handed down from generation to generation, provides a largely unexplored source for the potential development of active ingredients for cosmetic formulations. Therefore, the present comprehensive survey was conducted to identify cosmetic potential medicinal plants species in Sri Lanka. Personal interviews were conducted via a semistructured questionnaire with randomly selected 30 traditional practitioners and 90 Ayurveda physicians in Sri Lanka. Data were collected on plants and specific plant parts used for the treatment of skin care, hair care, and oral care topically. The acquired data were verified using the Ayurveda authentic books and quantitatively analyzed using relative frequency of citation (RFC), use value (UV), relative importance (RI), and factor informant consensus (FIC). Results revealed about the usage of 133 different plant species belonging to 64 families in cosmetic treatments under the categories of skin care, hair care, and oral care. Majority of medicinal plants were used in skin care treatments (39%) followed by hair care (20%) and oral care (17%).Aloe vera (L.) Burm.f. reported the highest RFC value (0.83) and UV (3.66). The highest RI value was reported from Asparagus racemosus Willd. and Hibiscus rosa-sinensis L. (1.67). The dominant plant family was reported as family Fabaceae. The most utilized plant part was stated as leaves (34%) followed by bark (14%). The survey further revealed about treatments for 17 skin-related, 9 hair-related, and 2 oral-related beauty issues. All RFC values were comparatively high for identified different beauty issues. Many herbal preparations were prepared using water as the medium whilst most common mode of application was reported as paste (37%). In conclusion, acquired information could ultimately be utilized for the development of the herbal cosmetic industry through the isolation and characterization of bioactive compounds from the documented plants while preserving the traditional knowledge.


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## Body

## 1. Introduction

Sri Lanka, formerly known as Ceylon, is an island with an area of approximately 65,610 km2. Despite its relatively small size, Sri Lanka possesses a high level of biodiversity due to its varied climate and topographical conditions [1]. As one of the most biologically diverse countries in Asia, Sri Lanka currently has 29.7% of forest cover [2] and 4,143 plant species distributed within 214 families. Of these, 1,025 plant species are endemic to the country [3]. In view of that, Sri Lanka is recognized as a biodiversity hotspot of global and national importance.Plants have been used for treating various illnesses over thousand years through four systems of traditional medicine in Sri Lanka calledAyurveda, Siddha, Unani, and Deshiya Chikitsa [4]. Traditional systems of medicine play a pivotal role in lives of Sri Lankan rural population by fulfilling 60–70% of primary health care needs [5]. According to the Sugathadasa et al. [6], 1,430 species representing 181 families and 838 genera are considered as medicinal plants. Out of the total number of medicinal plant species, 174 (12%) are endemic to the country. As described by Pushpakumara et al. [1], 250 species of medicinal plants are commonly used in traditional medicine of which 50 species are heavily used. Thus, it clearly implies the importance of medicinal plants in different systems of medicine in Sri Lanka.Medicinal plants have been used for centuries in medicinal, therapeutic, and beauty applications in Sri Lanka, which has documented history of over 2,500 years. However, many formulae for medicinal preparations of Sri Lankan traditional system of medicine are handed down from generation to generation or are found only in the scripts of old “ola leaf” books treasured by traditional and Ayurvedic practitioners [7]. Furthermore, the study on “Medicinal Plant Research in Sri Lanka: A Scientometric Study Based on Scopus Database,” highlights about the research studies of 190 plants including 22 endemic plants. It reveals that most of the conducted studies are activity-based studies such as toxicity, antibacterial, antifungal, hypoglycemic, antioxidant, anti-inflammatory, and diuretic. This is followed by general studies such as physicochemical, chemical, postharvest, horticultural, and propagation studies of plants [8]. These provide evidence for a largely unexplored knowledge gap of medicinal plants in Sri Lanka.Apart from the studies on “Cosmetic Perspective of Ethnobotany in Northern Part of Sri Lanka” [9], there has been hardly any ethnobotany report on cosmetic potential of Sri Lankan medicinal plants. A total of 62 plant species belonging 36 families have been identified based on the traditional knowledge and practices of local community through this study. Identified plants are used for beautifying purposes such as skin care, hair care, nail care, lip care, and eye care. Recently conducted few handfuls of research studies highlight about several medicinal plants with cosmetic potential. Napagoda et al. [10] highlights the probable usage of Atalantia ceylanica (Arn.) Oliver, Hibiscus furcatus Mullend., Leucas zeylanica (L.) W.T.Aiton, Mollugo cerviana (L.) Ser., Olax zeylanica L., and Ophiorrhiza mungos L. for the development of photoprotective cosmetic products via analyzing antioxidant activity and the sun protection factor (SPF). Moreover, research study conducted by Liyanaarachchi et al. [11] emphasizes about possible usage of Artocarpus nobilis Thw., Artocarpus altilis (Parkinson) Fosberg, Elaeocarpus serratus Heyne, Curcuma aromatica, and Artocarpus heterophyllus Lam. in the treatment of various skin disorders such as hyperpigmentation, to obtain lighter skin complexion, wrinkling, premature aging, and biological aging by analyzing tyrosinase, elastase, and hyaluronidase enzyme inhibitory and antioxidant activities.Nevertheless, there are only handfuls of scientific evidence available on bioactivity studies of medicinal plants in Sri Lanka that could lead to the development of herbal cosmetics [10]. Thus, it is a necessary requirement of the country to investigate the medicinal plants which exhibit cosmetic potential to support herbal cosmetic productions, innovations, and bioactivity studies while preserving the existing knowledge. In order to fulfil this knowledge gap, the present survey has focused to identify plants and plant parts used in topical cosmetic treatments of Sri Lanka under the categories of skin care, hair care, and oral care by interviewing traditional practitioners and Ayurveda physicians. Furthermore, major beauty issues treated by practitioners and mode of treatment applications have also been studied. Thereby, we believe the present study will support the development of the herbal cosmetic industry of Sri Lanka while rationalizing the ethnopharmacological usage.


## 2. Materials and Methods

### 2.1. Study Area and Selection of Respondents

This survey was conducted from January 2018 to August 2019. Ayurveda physicians and traditional practitioners engaged in cosmetic treatments were only considered. Names of the expert practitioners were listed by visiting the Ayurvedic Medical Council and from 09 provincial Ayurveda Departments (Northern Province, North Western Province, Western Province, Central Province, Uva Province, Sabaragamuwa Province, Eastern Province, Southern Province, and North Central Province), Sri Lanka (Figure1). In total, 210 expert practitioners (163 Ayurveda physicians and 47 traditional practitioners) were identified. Prior to data collection, each respondent was informed of the objectives of the study in order to obtain their consent and cooperation for the survey. However, permission was received only from 90 Ayurveda physicians and 30 traditional practitioners (Table 1). The total respondent percentage was 57%.Figure 1
Locations of the provincial Ayurveda Departments in Sri Lanka.Table 1
Distribution of respondents in 9 provinces of Sri Lanka.
ProvinceRespondentsAyurveda PhysiciansTraditional PractitionersNorthern92North Western115Western163Central93Uva81Sabaragamuwa94Eastern71Southern114North Central107Total9030120


### 2.2. Preparation of Questionnaire

Firstly, the questionnaire (Supplementary Material1) was pretested by interviewing 20 practitioners prior to the formal survey to understand the effectiveness of the questionnaire. Information was collected via a semistructured questionnaire under two main sections. The first section was designed to gather general information about the practitioners including practitioner’s name, gender, age, experience of the profession, level of education, address, province/district, and registration number assigned by the Ayurveda Department of Sri Lanka.The second section of the questionnaire was mainly dedicated to gather the information on medicinal plants and plant parts used in cosmetic treatments. Major beauty issues treated by practitioners and application mode of treatments were also studied. However, information on cosmetic remedies was not collected due to the unwillingness of practitioners to disclose their family recipes. In addition, suggestions for improving the research based on medicinal plants in cosmetics were recorded. Data collection was done by personal interviews.


### 2.3. Taxonomical Studies and Plant Specimens

Collected plants/plant materials from the practitioners were dried, preserved, and mounted on herbarium sheets. Herbarium voucher numbers were coded from NGHC 01 to NGHC 133 as the same order of the plant list indicated in Table2. Plant identification was carried out by comparing the deposited herbarium of the royal botanical garden, Peradeniya, Sri Lanka. The scientific names of plants were validated based on the collections listed in the homepage: http://www.theplantlist.org. The acquired data including vernacular names, English names, and remedies were verified using the Ayurveda authentic books, “Compendium of Medicinal Plants, A Sri Lankan Study,” volume I to IV issued by Ayurveda Department of Sri Lanka [12–15] and “A Collection of Medicinal Plants in Sri Lanka,” issued by Nature’s Beauty Creations Limited, Sri Lanka [16].Table 2
Medicinal plants used in topical cosmetic treatments of Sri Lanka.
No.FamilyScientific nameEnglish nameVernacular namePlant partPotential usageTreatment/s forMode of applicationRFCUVRI1AcanthaceaeBarleria lupulina Lindl.Hophead Philippine violetRanwan katu karanduLeavesSPIJU0.090.090.042Barleria prionitis L.CrossandraKatu karanduLeavesS, HSH, GHJU0.110.160.063Justicia adhatoda L.∗Malabar nutAdhatodaLeavesOOHOT0.420.420.204AcoraceaeAcorus calamus L.Sweet flagWada-kahaRhizomeS, HPE, SC, FAPA0.621.050.365AmaranthaceaeAlternanthera sessilis (L.) R.Br. ex DC∗Sessile joyweedMukunuwennaLeaves, stemHHHJU0.300.300.146AmaryllidaceaeAllium sativum L.GarlicSudulunuBulbSPIPA0.310.320.157AnnonaceaeAnnona muricata L.Prickly custard appleKatu-anodaTender leavesHPEJU0.280.280.138ApiaceaeCentella asiatica (L.) Urb∗Indian pennywortGotukolaEntire plantS, HDH, HEPA0.711.270.429ApocynaceaeCryptolepis dubia (Burm.f.) M.R.AlmeidaWel rukkattanaLeaves, bark, flowersS, OMM, MBDE0.080.120.0410Hemidesmus indicus (L.) R. Br. ex Schult.∗Indian sarsaparillaIramusuLeavesS, HHF, SCJU0.660.920.3511Holarrhena antidysenterica (Roxb.) Wall.KurchiKelindaBarkOOHDE0.200.200.1012ArecaceaeAreca catechu L.Areca nutPuwakFlowersOOHDE0.080.080.0413Caryota urens L.Wine palmKithulBark, tender flowersHHHOT0.060.070.0314Cocos nucifera L.∗King coconut/PolThambili/CoconutFruit kernelHHHOI0.630.630.3015AsparagaceaeAsparagus racemosus Willd.∗Wild asparagusHathawariyaEntire plantS, HHC, HH. HF, GH, AG, SC, FRJU0.602.200.5116AsphodelaceaeAloe vera (L.) Burm.f.∗Aloe plantKomarikaLeaf gelS, HFR, PI, HF, DN, AG, EXGE0.833.660.8017AsteraceaeEclipta prostrata (L.) L∗False daisyKeekirindiyaLeavesS, HHH, SD, AGOI, JU0.681.090.3818Vernonia anthelmintica (L.) Willd.Purple fleebaneSanninayamSeedsS, HFR, PEPA0.010.020.0119BerberidaceaeBerberis aristata DC.Indian barberryDaruharidraBarkOOHDE0.020.020.0120BombacaceaeBombax ceiba L.Red silk cotton treeImbulThrones, seedsSSC, FR, PIPA, OI0.050.080.0321BoraginaceaeHeliotropium indicum L.Indian heliotropeEthhodaLeavesSPIJU0.030.030.0122BrassicaceaeBrassica juncea (L.) CzernIndian mustardAbaSeedsSSS, SCOI0.360.530.2023CalophyllaceaeMesua ferrea L.∗Iron woodNaFlower stamensSMB, FR, SC, FAPA0.240.360.1324CannabaceaeCeltis timorensis Span.StinkwoodGurenda/BurendaWoodSDR, SCPA0.020.030.0125CaricaceaeCarica papaya L.∗PapayaGaslabuRipen fruitSFRPA0.610.610.2926CelastraceaeCelastrus paniculatus Willd.Black oil plantDuhuduBarkHPEPA0.030.030.0127Kokoona zeylanica Thwaites∗KokunBarkSSC, PIPO0.731.370.4428ClusiaceaeGarcinia mangostana L.MangosteenMangusBarkOOHDE0.090.090.0429Garcinia quaesita Pierre.∗Red mangoGorakaFruit rind, barkOOHOT0.290.280.1430CombretaceaeTerminalia arjuna (Roxb.) Wight & Arn.Arjuna myrobalanKumbukBarkS, OPI, OHPA0.180.190.0931Terminalia bellirica (Gaertn.) Roxb.∗Beleric myrobalanBuluSeedsHHC, HFOI0.721.120.4032Terminalia chebula Retz.∗MyrabalansAraluFruitS, OOH, AGIN0.701.030.3833ConvolvulaceaeArgyreia populifolia ChoisySri Lankan elephant creeperGiritillaShootsOOHJU0.050.050.0234Cuscuta chinensis Lam.DodderAgamula nathi walEntire plantHHC, HF, DNIN0.010.030.0135Evolvulus alsinoides (L.) L.Slender dwarf morning- gloryVishnukranthiEntire plantHHHOI0.070.080.0436Ipomoea pes-caprae (L.) R. Br.Goats foot creeperBinthamburuLeavesSFHDE0.020.020.0137CostaceaeCostus speciosus (Koenig) SmithCrape gingerThebuBarkSFROT0.010.010.0038CucurbitaceaeCucumis melo L.Bitter cucumberGon kakiriFruitSPIPA0.010.010.0039Cucumis sativus L.∗CucumberPipingnaFruitSHESA0.650.650.3140Trichosanthes cucumerina L.Wild sankegourdDummallaLeavesHGHDE0.030.030.0141CyperaceaeCyperus rotundus L.∗NutgrassKalanduruTuberSACPA0.540.530.2642DilleniaceaeDillenia retusa Thunb.∗GodaparaFruitHHH, HCLPA0.641.000.3643EbenaceaeDiospyros malabarica (Desr.) Kostel.Riber ebonyTimbiriFruitOOHDE0.010.010.0044ElaeocarpaceaeElaeocarpus serratus L.∗Wild oliveWeraluLeavesHHCLIN0.720.730.3445EuphorbiaceaeJatropha curcas LPurging nutRata endaruBarkOOHDE0.080.080.0446FabaceaeAbrus precatorius L.Wild liquoriceOlindaSeedsSACPA0.270.270.1347Acacia concinna (Willd.) DC.SeeniddaPodsHHH, DN, PE, HCLPO0.010.030.0148Acacia nilotica (L.) DelileGum Arabic treeBabbulaLeaves, bark, stemS, ODR, OHPA0.010.020.0149Adenanthera pavonina L.∗Red lucky seedMadatiyaLeavesSPI, SCJU0.300.430.1650Caesalpinia bonduc (L.) Roxb.Molucca beanKumburuSeedsS, OPI, OHOI, PO0.020.030.0151Caesalpinia sappanL.SappanwoodPatagiHeartwoodSWH, ACPO0.050.080.0352Crotalaria verrucosa L.Blue rattle weedNil-adanahiriyaLeaves, shootsOOHIN0.010.010.0053Entada rheedii Spreng.Elephant creepes mackayPus-welSeeds, bark, stemS, HPI, HCLPA0.040.030.0254Glycyrrhiza glabraL.∗LiqouriceWalmeeRootsSSCOT0.520.520.2555Indigofera tinctoria L.∗IndigoNil-awariLeavesS, HSC, HF, HHOI, PA0.601.300.3956Mimosa pudica L.Sensitive plantNidi-kumbaEntire plantS, OHE, OHPA, DE0.100.140.0557Pongamia pinnata (L.) Pierre∗Indian beechKarandaSeedsSSDOI0.120.120.0658Pterocarpus marsupium Roxb.Indian kino treeGammaluGumOOHOT0.010.010.0059Pterocarpus santalinus L.f.∗Red sandalwoodRath-handunHeartwoodSPIPA0.670.670.3260Saraca asoca(Roxb.) Willd.Ashoka treeAsokaBarkOMM, OHPO0.010.020.0161Senna alata (L.) Roxb.∗Candle bushEththoraLeavesSSDPA0.540.530.2662Senna alexandrina Mill.Indian sennaSenehekolaLeavesHDN, HHDE0.020.030.0163Senna auriculata (L.) Roxb.∗Tanner’s cassiaRanawaraFlowersSSCPA0.150.150.0764Sesbania grandiflora (L.) Pers.∗Vegetable hummingbirdKathurumurungaLeavesHHHOI0.470.480.2365Tephrosia purpurea (L.) Pers.∗Purple tephrosiaKathurupilaRootsOOHOT0.730.730.3566Trigonella foenum-graecum L.∗FenugreekUluhalSeedsHDNIN0.640.640.3067HypoxidaceaeCurculigo orchioides Gaertn.Black musaleBinthalTuberSSCPA0.010.010.0068LamiaceaeOcimum tenuiflorum L.∗Holy basilHeen maduruthalaLeavesSFA, PI, FROI, JU0.190.320.1169Pogostemon heyneanus Benth.PatchouliGas-kollankolaLeavesOOHIN0.010.010.0070Premna obtusifolia R.Br.Heen midiLeavesHHCLJU0.010.010.0071Tectona grandis L.f.TeakThekkaFruitHHHPO0.010.010.0072LauraceaeCinnamomum verum J.Presl∗Ceylon cinnamomKurunduInner barkS, OPI, OHPA0.600.990.3473LecythidaceaeBarringtonia acutangula (L.) Gaertn.Indian oakMidellaLeaves, bark, flowerOOHDE0.010.010.0074Careya arborea Roxb.Patana oakKahataBark gumSSSOT0.010.010.0075Couroupita guianensis Aubl.Cannon ball treeSalLeavesSHEOT0.010.010.0076LythraceaeLawsonia inermis L.∗HennaMarathondiLeavesHGH, HHIN0.440.640.2477Punica granatum L.∗PomegranateDelumLeaves, fruit rind, rootsS, OOHDE0.070.080.0478MagnoliaceaeMichelia champaca var. blumei MoritziChampakGini sapuFlowersSFA, HEOI0.050.080.0379MalvaceaeAbutilon indicum (L.) SweetCountry mallowBehethanodaLeavesOOHPA0.010.010.0080Gossypium arboreum L.CottonKapuLeavesSHEOT0.060.070.0381Hibiscus abelmoschus L.∗Musk mallowKapukinissaSeedsS, OMM, FAPA0.400.630.2282Hibiscus rosa-sinensis L.∗Shoe flowerPokuru wadaLeaves, flowersS, HHH, GH, HF, DN, SD, PI, FROI, PA0.090.410.0983Sida cordata (Burm.f.) Borss. Waalk∗Heart leaf sidaWel bebilaLeaves, stemS, HHH, PIPA0.160.230.0884Theobroma cacao L.∗CocoKokovaSeedsSDR, SSOT0.731.090.4085MeliaceaeAzadirachta indica A.Juss∗NeemKohombaLeaves, rootsS, OPI, OHPA, DE0.741.220.4286MenispermaceaeCoscinium fenestratum (Goetgh.) Colebr.∗Calumba woodWeniwelStemSEX, ACPA0.741.270.4387MoraceaeFiscus racemosa L.∗Country figAttikkaLeavesSFR, SDPA0.460.650.2588MoringaceaeMoringa oleifera Lam.∗Drumstick treeMurungaLeavesSPI, FR, SS, SHJU, PA0.070.130.0489MyristicaceaeMyristica fragrans Houtt.∗NutmegSadhikkaSeedsS, H, OPE, HCL, OHOI0.691.530.4590MyrtaceaeMelaleuca leucadendra (L.) L.∗Cajuput treeLothsumbuluBarkSSCOT0.520.520.2591Psidium guajava L.∗GuavaPeraLeavesHHHJU0.730.730.3592Syzygium aomaricum (L.) Merr. & L.M.Perry∗CloveKarabuFlower budsOOHOT0.090.090.0493Syzygium cumini (L.) SkeelsIndian black berryMadanBarkOOHOT0.140.140.0794NymphaeaceaeNymphaea nouchali Burm.f∗Blue water lilyNil manelFlowersHHCPA0.320.330.1595OleaceaeJasminum grandiflorum L.∗JasmineSamanpichchaFlowers, leaves, rootsS, HSD, FA, SCOPA, OI0.190.360.1196Jasminum multiflorum (Burm.f.) AndrewsSambac JasmineGeta pichchaFlower budsSSCPA0.060.070.0397OxalidaceaeAverrhoa bilimbi L.BilimbiBilinLeavesS, HPI, PEJU0.560.790.3098PedaliaceaeSesamum indicum L.∗GingellyThel-thalaSeedsS, HHH, HEOT0.701.120.3999PhyllanthaceaePhyllanthus emblica L.∗Emblic myrobalanNelliLeaves, fruitS, HSC, HF, GH,OT0.180.380.11100PinaceaeCedrus deodara (Roxb. ex D.Don) G.Don∗Himalayan cedarDewadaraHeartwoodSFAOT0.240.240.11101PiperaceaePiper nigrum L.∗PepperGammirisSeedsS, OFAOT0.580.580.28102PlantaginaceaeBacopa monnieri (L.) Wettst.∗Thyme leaved gratiolaLunuwilaEntire plantS, HAG, DNOT0.160.250.09103PoaceaeCymbopogon citratus (DC.) Stapf∗Lemon grassSeraEntire plantHPE, DN, FAOI0.721.130.40104Vetiveria zizanioides (L) Nash∗Khas-khasSawandaraRootsSFAOI, PA0.700.700.33105PonterderiaceaeMonochoria vaginalis (Burm.f.) C. Presl∗Oval leafed pondweedDiyahabaralaRootsOOHIN0.180.180.08106RubiaceaeCoffea arabicaL.∗CoffeeKopiSeedsSEXPO0.120.120.06107Geophila repens(L.) I.M.Johnst.KothurubeddaLeavesSSDPA0.010.010.00108Morinda citrifolia L.Indian mulberryAhuLeaves, fruitS, OOH, EX, HEJU, PA0.150.260.09109Oldenlandia corymbosaL.Diamond flowerPathpdagamEntire plantSPIOT0.010.010.00110Rubia cordifolia L.∗Heart leaved madderVelmadataRootsSFR, SDPA0.430.630.23111RutaceaeAcronychia pedunculata (L.) Miq.∗Claw flowered laurelAnkendaLeavesSPIPA0.390.390.19112Aegle marmelos (L.) Correa∗Bael fruit treeBeliRipen fruitSSCPA0.530.530.25113Citrus aurantifolia Panzer. (Christm.) Swingle∗True limeDehiFruitS, HHCL, SCL, DN, PEIn, PA0.591.300.38114Citrus hystrix DC.Kaffir limeGada dehiFruitHDN, FAJU0.130.200.07115Citrus limon (L.) Osbeck∗LemonLemonFruitSSCL, DRJU0.691.100.39116Melicope lunu-ankenda (Gaertn.) T.G. HartleyLunu-ankendaLeavesSSCPA0.020.020.01117Murraya koenigii (L.) Spreng.Curry leafKarapinchaLeavesHHHJU0.500.500.24118Ruta graveolens L.∗Garden rueArudaLeavesHHHOI0.520.520.25119SantalaceaeSantalum album L.∗SandalwoodSudu handunHeartwoodSPI, FR, SC, AGPA0.741.910.52120SapindaceaeSapindus mukorossi Gaertn.∗Soap nutGas penelaFruitHHCLPA0.070.080.04121Schleichera oleosa (Lour.) Merr.∗Ceylon oakKonSeedsHHHOI0.190.190.09122SapotaceaeMimusops elengi L.∗Bullet wood treeMunamalBarkOOHDE0.690.690.33123SolanaceaeDatura metel L.DaturaAttanaRootsOOHPO0.010.010.00124Withania somnifera(L.) Dunal∗Indian ginsengAmukkraRootsSAGOT0.040.030.02125SymplocaceaeSymplocos cochinchinensis (Lour.) S. MooreBombuBarkOOHDE0.010.010.00126TheaceaeCamellia sinensis (L.) Kuntze∗TeaThaeTender leavesSWROT0.130.130.06127VitaceaeLeea indica (Burm.f.) Merr.Bandicoot berryGurullaLeavesSSD, SCPA0.010.020.01128ZingiberaceaAlpinia malaccensis (Burm.f.) Roscoe∗RankihiriyaFlower budsOOHJU0.010.010.00129Curcuma aromatic Salisb.∗Wild turmericKasthuri kahaRhizomeSSCPA0.670.670.32130Curcuma longa L.∗TurmericAth kahaRhizomeSSCPA0.700.700.33131Curcuma zedoaria (christm.) Roscoe∗ZedoaryHaran kahaRhizomeSSC, FAPA0.050.080.03132Kaempferia galanga L.Java galangaIngurupiyaliRhizomeS, H, OOH, SC, DN, WHIn, PA0.250.650.18133Zingiber officinale Roscoe∗GingerInguruRhizomeSPI, SCJU0.230.350.13Note. RFC: relative frequency of citation; UV: use value; RI: relative importance; S: skin care; H: hair care; O: oral care; OH: oral hygiene; MM: malodour of the mouth; HH: healthy hair; DN: dandruff; PE: pediculosis; HCL: scalp and hair cleansing; DH: damaged hair; HF: hair fallen; GH: grey hair; HC: hair color; SCO: scalp cooling; SC: skin complexion; PI: pimples; FR: freckles; HE: healing; AG: aging; SD: skin discoloration; AC: acne; DR: skin dryness; EX: exfoliate; SS: soften the skin; INF: infections; MB: malodour of the body; SCL: skin cleansing; SH: skin health; WH: whitening; WR: wrinkles; FA: fragrant agent; FH: foot health; PA: paste; JU: juice; OI: oil; DE: decoction; PO: powder; IN: infusion; GE: gel; SA: salad; OT: others. ∗ Plants used in commercial herbal cosmetic products in Sri Lanka.


### 2.4. Quantitative Analysis of Information

The traditional knowledge of medicinal plant usage in cosmetic treatments was quantitatively analyzed using the relative frequency of citation, use value, factor informant consensus, and relative importance as standard methods described by Hoffman and Gallaher, [17] and Vitalini et al. [18].


#### 2.4.1. Relative Frequency of Citation (RFC)

RFC determined the agreement among the informants on the use of medicinal plants. Furthermore, this index shows the local importance of each species. The value of RFC for species is based on the citing percentage of informants for that particular plant species [19]. The RFC was calculated using following formula:(1)RFC=FCN0<RFC<1,where “FC” is the number of informants mentioned the species while “N” indicates the total number of informants participated in the survey.


#### 2.4.2. Use Value (UV)

UV is a good indicator to estimate all the possible uses of a plant species without considering its RFC. It demonstrates the relative importance of plant species known locally, considering the number of uses mentioned by an informant. High values (>1∼) indicate the various use reports for a plant while near zero values highlight the less use reports. However, UV does not distinguish whether a plant is used for single or multiple purposes [20].This was calculated by following formula:(2)UV=∑UiN,where “Ui” is the total number of uses mentioned by each informant for a given species “i” and “N” is the total number of informants participated in the survey.


#### 2.4.3. Factor Informant Consensus (FIC)

FIC indicates the homogeneity of the information on the use of plants to treat the different types of beauty issues. Higher FIC values (higher when closer to 1) describe the well-defined selection criterion, and/or information is exchanged between informants. If informants do not exchange information about plants’ uses, FIC values will be near to zero [21].FIC was calculated as follows:(3)FIC=nur−ntnur−1,where “nur” depicts the number of use citations in each category while “nt” indicates the number of used taxa.


#### 2.4.4. Relative Importance (RI)

The following formula was used to calculate the RI:(4)RI=NUC+NT,where NUC = number of use categories of a given species/total number of use categories of the most versatile species. NT = types of uses attributed to a given species/total number of types of uses attributed to the most important taxon.


#### 2.4.5. Statistical Analysis

Descriptive statistics were used to present the collected data of the survey. Correlation analysis based on simple linear regression between RFC and UV, RFC and RI, and UV and RI was performed. Statistical significance was set at 5%. The programmes used were Microsoft Excel 2016 and Minitab 17.


## 2.1. Study Area and Selection of Respondents

This survey was conducted from January 2018 to August 2019. Ayurveda physicians and traditional practitioners engaged in cosmetic treatments were only considered. Names of the expert practitioners were listed by visiting the Ayurvedic Medical Council and from 09 provincial Ayurveda Departments (Northern Province, North Western Province, Western Province, Central Province, Uva Province, Sabaragamuwa Province, Eastern Province, Southern Province, and North Central Province), Sri Lanka (Figure1). In total, 210 expert practitioners (163 Ayurveda physicians and 47 traditional practitioners) were identified. Prior to data collection, each respondent was informed of the objectives of the study in order to obtain their consent and cooperation for the survey. However, permission was received only from 90 Ayurveda physicians and 30 traditional practitioners (Table 1). The total respondent percentage was 57%.Figure 1
Locations of the provincial Ayurveda Departments in Sri Lanka.Table 1
Distribution of respondents in 9 provinces of Sri Lanka.
ProvinceRespondentsAyurveda PhysiciansTraditional PractitionersNorthern92North Western115Western163Central93Uva81Sabaragamuwa94Eastern71Southern114North Central107Total9030120


## 2.2. Preparation of Questionnaire

Firstly, the questionnaire (Supplementary Material1) was pretested by interviewing 20 practitioners prior to the formal survey to understand the effectiveness of the questionnaire. Information was collected via a semistructured questionnaire under two main sections. The first section was designed to gather general information about the practitioners including practitioner’s name, gender, age, experience of the profession, level of education, address, province/district, and registration number assigned by the Ayurveda Department of Sri Lanka.The second section of the questionnaire was mainly dedicated to gather the information on medicinal plants and plant parts used in cosmetic treatments. Major beauty issues treated by practitioners and application mode of treatments were also studied. However, information on cosmetic remedies was not collected due to the unwillingness of practitioners to disclose their family recipes. In addition, suggestions for improving the research based on medicinal plants in cosmetics were recorded. Data collection was done by personal interviews.


## 2.3. Taxonomical Studies and Plant Specimens

Collected plants/plant materials from the practitioners were dried, preserved, and mounted on herbarium sheets. Herbarium voucher numbers were coded from NGHC 01 to NGHC 133 as the same order of the plant list indicated in Table2. Plant identification was carried out by comparing the deposited herbarium of the royal botanical garden, Peradeniya, Sri Lanka. The scientific names of plants were validated based on the collections listed in the homepage: http://www.theplantlist.org. The acquired data including vernacular names, English names, and remedies were verified using the Ayurveda authentic books, “Compendium of Medicinal Plants, A Sri Lankan Study,” volume I to IV issued by Ayurveda Department of Sri Lanka [12–15] and “A Collection of Medicinal Plants in Sri Lanka,” issued by Nature’s Beauty Creations Limited, Sri Lanka [16].Table 2
Medicinal plants used in topical cosmetic treatments of Sri Lanka.
No.FamilyScientific nameEnglish nameVernacular namePlant partPotential usageTreatment/s forMode of applicationRFCUVRI1AcanthaceaeBarleria lupulina Lindl.Hophead Philippine violetRanwan katu karanduLeavesSPIJU0.090.090.042Barleria prionitis L.CrossandraKatu karanduLeavesS, HSH, GHJU0.110.160.063Justicia adhatoda L.∗Malabar nutAdhatodaLeavesOOHOT0.420.420.204AcoraceaeAcorus calamus L.Sweet flagWada-kahaRhizomeS, HPE, SC, FAPA0.621.050.365AmaranthaceaeAlternanthera sessilis (L.) R.Br. ex DC∗Sessile joyweedMukunuwennaLeaves, stemHHHJU0.300.300.146AmaryllidaceaeAllium sativum L.GarlicSudulunuBulbSPIPA0.310.320.157AnnonaceaeAnnona muricata L.Prickly custard appleKatu-anodaTender leavesHPEJU0.280.280.138ApiaceaeCentella asiatica (L.) Urb∗Indian pennywortGotukolaEntire plantS, HDH, HEPA0.711.270.429ApocynaceaeCryptolepis dubia (Burm.f.) M.R.AlmeidaWel rukkattanaLeaves, bark, flowersS, OMM, MBDE0.080.120.0410Hemidesmus indicus (L.) R. Br. ex Schult.∗Indian sarsaparillaIramusuLeavesS, HHF, SCJU0.660.920.3511Holarrhena antidysenterica (Roxb.) Wall.KurchiKelindaBarkOOHDE0.200.200.1012ArecaceaeAreca catechu L.Areca nutPuwakFlowersOOHDE0.080.080.0413Caryota urens L.Wine palmKithulBark, tender flowersHHHOT0.060.070.0314Cocos nucifera L.∗King coconut/PolThambili/CoconutFruit kernelHHHOI0.630.630.3015AsparagaceaeAsparagus racemosus Willd.∗Wild asparagusHathawariyaEntire plantS, HHC, HH. HF, GH, AG, SC, FRJU0.602.200.5116AsphodelaceaeAloe vera (L.) Burm.f.∗Aloe plantKomarikaLeaf gelS, HFR, PI, HF, DN, AG, EXGE0.833.660.8017AsteraceaeEclipta prostrata (L.) L∗False daisyKeekirindiyaLeavesS, HHH, SD, AGOI, JU0.681.090.3818Vernonia anthelmintica (L.) Willd.Purple fleebaneSanninayamSeedsS, HFR, PEPA0.010.020.0119BerberidaceaeBerberis aristata DC.Indian barberryDaruharidraBarkOOHDE0.020.020.0120BombacaceaeBombax ceiba L.Red silk cotton treeImbulThrones, seedsSSC, FR, PIPA, OI0.050.080.0321BoraginaceaeHeliotropium indicum L.Indian heliotropeEthhodaLeavesSPIJU0.030.030.0122BrassicaceaeBrassica juncea (L.) CzernIndian mustardAbaSeedsSSS, SCOI0.360.530.2023CalophyllaceaeMesua ferrea L.∗Iron woodNaFlower stamensSMB, FR, SC, FAPA0.240.360.1324CannabaceaeCeltis timorensis Span.StinkwoodGurenda/BurendaWoodSDR, SCPA0.020.030.0125CaricaceaeCarica papaya L.∗PapayaGaslabuRipen fruitSFRPA0.610.610.2926CelastraceaeCelastrus paniculatus Willd.Black oil plantDuhuduBarkHPEPA0.030.030.0127Kokoona zeylanica Thwaites∗KokunBarkSSC, PIPO0.731.370.4428ClusiaceaeGarcinia mangostana L.MangosteenMangusBarkOOHDE0.090.090.0429Garcinia quaesita Pierre.∗Red mangoGorakaFruit rind, barkOOHOT0.290.280.1430CombretaceaeTerminalia arjuna (Roxb.) Wight & Arn.Arjuna myrobalanKumbukBarkS, OPI, OHPA0.180.190.0931Terminalia bellirica (Gaertn.) Roxb.∗Beleric myrobalanBuluSeedsHHC, HFOI0.721.120.4032Terminalia chebula Retz.∗MyrabalansAraluFruitS, OOH, AGIN0.701.030.3833ConvolvulaceaeArgyreia populifolia ChoisySri Lankan elephant creeperGiritillaShootsOOHJU0.050.050.0234Cuscuta chinensis Lam.DodderAgamula nathi walEntire plantHHC, HF, DNIN0.010.030.0135Evolvulus alsinoides (L.) L.Slender dwarf morning- gloryVishnukranthiEntire plantHHHOI0.070.080.0436Ipomoea pes-caprae (L.) R. Br.Goats foot creeperBinthamburuLeavesSFHDE0.020.020.0137CostaceaeCostus speciosus (Koenig) SmithCrape gingerThebuBarkSFROT0.010.010.0038CucurbitaceaeCucumis melo L.Bitter cucumberGon kakiriFruitSPIPA0.010.010.0039Cucumis sativus L.∗CucumberPipingnaFruitSHESA0.650.650.3140Trichosanthes cucumerina L.Wild sankegourdDummallaLeavesHGHDE0.030.030.0141CyperaceaeCyperus rotundus L.∗NutgrassKalanduruTuberSACPA0.540.530.2642DilleniaceaeDillenia retusa Thunb.∗GodaparaFruitHHH, HCLPA0.641.000.3643EbenaceaeDiospyros malabarica (Desr.) Kostel.Riber ebonyTimbiriFruitOOHDE0.010.010.0044ElaeocarpaceaeElaeocarpus serratus L.∗Wild oliveWeraluLeavesHHCLIN0.720.730.3445EuphorbiaceaeJatropha curcas LPurging nutRata endaruBarkOOHDE0.080.080.0446FabaceaeAbrus precatorius L.Wild liquoriceOlindaSeedsSACPA0.270.270.1347Acacia concinna (Willd.) DC.SeeniddaPodsHHH, DN, PE, HCLPO0.010.030.0148Acacia nilotica (L.) DelileGum Arabic treeBabbulaLeaves, bark, stemS, ODR, OHPA0.010.020.0149Adenanthera pavonina L.∗Red lucky seedMadatiyaLeavesSPI, SCJU0.300.430.1650Caesalpinia bonduc (L.) Roxb.Molucca beanKumburuSeedsS, OPI, OHOI, PO0.020.030.0151Caesalpinia sappanL.SappanwoodPatagiHeartwoodSWH, ACPO0.050.080.0352Crotalaria verrucosa L.Blue rattle weedNil-adanahiriyaLeaves, shootsOOHIN0.010.010.0053Entada rheedii Spreng.Elephant creepes mackayPus-welSeeds, bark, stemS, HPI, HCLPA0.040.030.0254Glycyrrhiza glabraL.∗LiqouriceWalmeeRootsSSCOT0.520.520.2555Indigofera tinctoria L.∗IndigoNil-awariLeavesS, HSC, HF, HHOI, PA0.601.300.3956Mimosa pudica L.Sensitive plantNidi-kumbaEntire plantS, OHE, OHPA, DE0.100.140.0557Pongamia pinnata (L.) Pierre∗Indian beechKarandaSeedsSSDOI0.120.120.0658Pterocarpus marsupium Roxb.Indian kino treeGammaluGumOOHOT0.010.010.0059Pterocarpus santalinus L.f.∗Red sandalwoodRath-handunHeartwoodSPIPA0.670.670.3260Saraca asoca(Roxb.) Willd.Ashoka treeAsokaBarkOMM, OHPO0.010.020.0161Senna alata (L.) Roxb.∗Candle bushEththoraLeavesSSDPA0.540.530.2662Senna alexandrina Mill.Indian sennaSenehekolaLeavesHDN, HHDE0.020.030.0163Senna auriculata (L.) Roxb.∗Tanner’s cassiaRanawaraFlowersSSCPA0.150.150.0764Sesbania grandiflora (L.) Pers.∗Vegetable hummingbirdKathurumurungaLeavesHHHOI0.470.480.2365Tephrosia purpurea (L.) Pers.∗Purple tephrosiaKathurupilaRootsOOHOT0.730.730.3566Trigonella foenum-graecum L.∗FenugreekUluhalSeedsHDNIN0.640.640.3067HypoxidaceaeCurculigo orchioides Gaertn.Black musaleBinthalTuberSSCPA0.010.010.0068LamiaceaeOcimum tenuiflorum L.∗Holy basilHeen maduruthalaLeavesSFA, PI, FROI, JU0.190.320.1169Pogostemon heyneanus Benth.PatchouliGas-kollankolaLeavesOOHIN0.010.010.0070Premna obtusifolia R.Br.Heen midiLeavesHHCLJU0.010.010.0071Tectona grandis L.f.TeakThekkaFruitHHHPO0.010.010.0072LauraceaeCinnamomum verum J.Presl∗Ceylon cinnamomKurunduInner barkS, OPI, OHPA0.600.990.3473LecythidaceaeBarringtonia acutangula (L.) Gaertn.Indian oakMidellaLeaves, bark, flowerOOHDE0.010.010.0074Careya arborea Roxb.Patana oakKahataBark gumSSSOT0.010.010.0075Couroupita guianensis Aubl.Cannon ball treeSalLeavesSHEOT0.010.010.0076LythraceaeLawsonia inermis L.∗HennaMarathondiLeavesHGH, HHIN0.440.640.2477Punica granatum L.∗PomegranateDelumLeaves, fruit rind, rootsS, OOHDE0.070.080.0478MagnoliaceaeMichelia champaca var. blumei MoritziChampakGini sapuFlowersSFA, HEOI0.050.080.0379MalvaceaeAbutilon indicum (L.) SweetCountry mallowBehethanodaLeavesOOHPA0.010.010.0080Gossypium arboreum L.CottonKapuLeavesSHEOT0.060.070.0381Hibiscus abelmoschus L.∗Musk mallowKapukinissaSeedsS, OMM, FAPA0.400.630.2282Hibiscus rosa-sinensis L.∗Shoe flowerPokuru wadaLeaves, flowersS, HHH, GH, HF, DN, SD, PI, FROI, PA0.090.410.0983Sida cordata (Burm.f.) Borss. Waalk∗Heart leaf sidaWel bebilaLeaves, stemS, HHH, PIPA0.160.230.0884Theobroma cacao L.∗CocoKokovaSeedsSDR, SSOT0.731.090.4085MeliaceaeAzadirachta indica A.Juss∗NeemKohombaLeaves, rootsS, OPI, OHPA, DE0.741.220.4286MenispermaceaeCoscinium fenestratum (Goetgh.) Colebr.∗Calumba woodWeniwelStemSEX, ACPA0.741.270.4387MoraceaeFiscus racemosa L.∗Country figAttikkaLeavesSFR, SDPA0.460.650.2588MoringaceaeMoringa oleifera Lam.∗Drumstick treeMurungaLeavesSPI, FR, SS, SHJU, PA0.070.130.0489MyristicaceaeMyristica fragrans Houtt.∗NutmegSadhikkaSeedsS, H, OPE, HCL, OHOI0.691.530.4590MyrtaceaeMelaleuca leucadendra (L.) L.∗Cajuput treeLothsumbuluBarkSSCOT0.520.520.2591Psidium guajava L.∗GuavaPeraLeavesHHHJU0.730.730.3592Syzygium aomaricum (L.) Merr. & L.M.Perry∗CloveKarabuFlower budsOOHOT0.090.090.0493Syzygium cumini (L.) SkeelsIndian black berryMadanBarkOOHOT0.140.140.0794NymphaeaceaeNymphaea nouchali Burm.f∗Blue water lilyNil manelFlowersHHCPA0.320.330.1595OleaceaeJasminum grandiflorum L.∗JasmineSamanpichchaFlowers, leaves, rootsS, HSD, FA, SCOPA, OI0.190.360.1196Jasminum multiflorum (Burm.f.) AndrewsSambac JasmineGeta pichchaFlower budsSSCPA0.060.070.0397OxalidaceaeAverrhoa bilimbi L.BilimbiBilinLeavesS, HPI, PEJU0.560.790.3098PedaliaceaeSesamum indicum L.∗GingellyThel-thalaSeedsS, HHH, HEOT0.701.120.3999PhyllanthaceaePhyllanthus emblica L.∗Emblic myrobalanNelliLeaves, fruitS, HSC, HF, GH,OT0.180.380.11100PinaceaeCedrus deodara (Roxb. ex D.Don) G.Don∗Himalayan cedarDewadaraHeartwoodSFAOT0.240.240.11101PiperaceaePiper nigrum L.∗PepperGammirisSeedsS, OFAOT0.580.580.28102PlantaginaceaeBacopa monnieri (L.) Wettst.∗Thyme leaved gratiolaLunuwilaEntire plantS, HAG, DNOT0.160.250.09103PoaceaeCymbopogon citratus (DC.) Stapf∗Lemon grassSeraEntire plantHPE, DN, FAOI0.721.130.40104Vetiveria zizanioides (L) Nash∗Khas-khasSawandaraRootsSFAOI, PA0.700.700.33105PonterderiaceaeMonochoria vaginalis (Burm.f.) C. Presl∗Oval leafed pondweedDiyahabaralaRootsOOHIN0.180.180.08106RubiaceaeCoffea arabicaL.∗CoffeeKopiSeedsSEXPO0.120.120.06107Geophila repens(L.) I.M.Johnst.KothurubeddaLeavesSSDPA0.010.010.00108Morinda citrifolia L.Indian mulberryAhuLeaves, fruitS, OOH, EX, HEJU, PA0.150.260.09109Oldenlandia corymbosaL.Diamond flowerPathpdagamEntire plantSPIOT0.010.010.00110Rubia cordifolia L.∗Heart leaved madderVelmadataRootsSFR, SDPA0.430.630.23111RutaceaeAcronychia pedunculata (L.) Miq.∗Claw flowered laurelAnkendaLeavesSPIPA0.390.390.19112Aegle marmelos (L.) Correa∗Bael fruit treeBeliRipen fruitSSCPA0.530.530.25113Citrus aurantifolia Panzer. (Christm.) Swingle∗True limeDehiFruitS, HHCL, SCL, DN, PEIn, PA0.591.300.38114Citrus hystrix DC.Kaffir limeGada dehiFruitHDN, FAJU0.130.200.07115Citrus limon (L.) Osbeck∗LemonLemonFruitSSCL, DRJU0.691.100.39116Melicope lunu-ankenda (Gaertn.) T.G. HartleyLunu-ankendaLeavesSSCPA0.020.020.01117Murraya koenigii (L.) Spreng.Curry leafKarapinchaLeavesHHHJU0.500.500.24118Ruta graveolens L.∗Garden rueArudaLeavesHHHOI0.520.520.25119SantalaceaeSantalum album L.∗SandalwoodSudu handunHeartwoodSPI, FR, SC, AGPA0.741.910.52120SapindaceaeSapindus mukorossi Gaertn.∗Soap nutGas penelaFruitHHCLPA0.070.080.04121Schleichera oleosa (Lour.) Merr.∗Ceylon oakKonSeedsHHHOI0.190.190.09122SapotaceaeMimusops elengi L.∗Bullet wood treeMunamalBarkOOHDE0.690.690.33123SolanaceaeDatura metel L.DaturaAttanaRootsOOHPO0.010.010.00124Withania somnifera(L.) Dunal∗Indian ginsengAmukkraRootsSAGOT0.040.030.02125SymplocaceaeSymplocos cochinchinensis (Lour.) S. MooreBombuBarkOOHDE0.010.010.00126TheaceaeCamellia sinensis (L.) Kuntze∗TeaThaeTender leavesSWROT0.130.130.06127VitaceaeLeea indica (Burm.f.) Merr.Bandicoot berryGurullaLeavesSSD, SCPA0.010.020.01128ZingiberaceaAlpinia malaccensis (Burm.f.) Roscoe∗RankihiriyaFlower budsOOHJU0.010.010.00129Curcuma aromatic Salisb.∗Wild turmericKasthuri kahaRhizomeSSCPA0.670.670.32130Curcuma longa L.∗TurmericAth kahaRhizomeSSCPA0.700.700.33131Curcuma zedoaria (christm.) Roscoe∗ZedoaryHaran kahaRhizomeSSC, FAPA0.050.080.03132Kaempferia galanga L.Java galangaIngurupiyaliRhizomeS, H, OOH, SC, DN, WHIn, PA0.250.650.18133Zingiber officinale Roscoe∗GingerInguruRhizomeSPI, SCJU0.230.350.13Note. RFC: relative frequency of citation; UV: use value; RI: relative importance; S: skin care; H: hair care; O: oral care; OH: oral hygiene; MM: malodour of the mouth; HH: healthy hair; DN: dandruff; PE: pediculosis; HCL: scalp and hair cleansing; DH: damaged hair; HF: hair fallen; GH: grey hair; HC: hair color; SCO: scalp cooling; SC: skin complexion; PI: pimples; FR: freckles; HE: healing; AG: aging; SD: skin discoloration; AC: acne; DR: skin dryness; EX: exfoliate; SS: soften the skin; INF: infections; MB: malodour of the body; SCL: skin cleansing; SH: skin health; WH: whitening; WR: wrinkles; FA: fragrant agent; FH: foot health; PA: paste; JU: juice; OI: oil; DE: decoction; PO: powder; IN: infusion; GE: gel; SA: salad; OT: others. ∗ Plants used in commercial herbal cosmetic products in Sri Lanka.


## 2.4. Quantitative Analysis of Information

The traditional knowledge of medicinal plant usage in cosmetic treatments was quantitatively analyzed using the relative frequency of citation, use value, factor informant consensus, and relative importance as standard methods described by Hoffman and Gallaher, [17] and Vitalini et al. [18].


### 2.4.1. Relative Frequency of Citation (RFC)

RFC determined the agreement among the informants on the use of medicinal plants. Furthermore, this index shows the local importance of each species. The value of RFC for species is based on the citing percentage of informants for that particular plant species [19]. The RFC was calculated using following formula:(1)RFC=FCN0<RFC<1,where “FC” is the number of informants mentioned the species while “N” indicates the total number of informants participated in the survey.


### 2.4.2. Use Value (UV)

UV is a good indicator to estimate all the possible uses of a plant species without considering its RFC. It demonstrates the relative importance of plant species known locally, considering the number of uses mentioned by an informant. High values (>1∼) indicate the various use reports for a plant while near zero values highlight the less use reports. However, UV does not distinguish whether a plant is used for single or multiple purposes [20].This was calculated by following formula:(2)UV=∑UiN,where “Ui” is the total number of uses mentioned by each informant for a given species “i” and “N” is the total number of informants participated in the survey.


### 2.4.3. Factor Informant Consensus (FIC)

FIC indicates the homogeneity of the information on the use of plants to treat the different types of beauty issues. Higher FIC values (higher when closer to 1) describe the well-defined selection criterion, and/or information is exchanged between informants. If informants do not exchange information about plants’ uses, FIC values will be near to zero [21].FIC was calculated as follows:(3)FIC=nur−ntnur−1,where “nur” depicts the number of use citations in each category while “nt” indicates the number of used taxa.


### 2.4.4. Relative Importance (RI)

The following formula was used to calculate the RI:(4)RI=NUC+NT,where NUC = number of use categories of a given species/total number of use categories of the most versatile species. NT = types of uses attributed to a given species/total number of types of uses attributed to the most important taxon.


### 2.4.5. Statistical Analysis

Descriptive statistics were used to present the collected data of the survey. Correlation analysis based on simple linear regression between RFC and UV, RFC and RI, and UV and RI was performed. Statistical significance was set at 5%. The programmes used were Microsoft Excel 2016 and Minitab 17.


## 2.4.1. Relative Frequency of Citation (RFC)

RFC determined the agreement among the informants on the use of medicinal plants. Furthermore, this index shows the local importance of each species. The value of RFC for species is based on the citing percentage of informants for that particular plant species [19]. The RFC was calculated using following formula:(1)RFC=FCN0<RFC<1,where “FC” is the number of informants mentioned the species while “N” indicates the total number of informants participated in the survey.


## 2.4.2. Use Value (UV)

UV is a good indicator to estimate all the possible uses of a plant species without considering its RFC. It demonstrates the relative importance of plant species known locally, considering the number of uses mentioned by an informant. High values (>1∼) indicate the various use reports for a plant while near zero values highlight the less use reports. However, UV does not distinguish whether a plant is used for single or multiple purposes [20].This was calculated by following formula:(2)UV=∑UiN,where “Ui” is the total number of uses mentioned by each informant for a given species “i” and “N” is the total number of informants participated in the survey.


## 2.4.3. Factor Informant Consensus (FIC)

FIC indicates the homogeneity of the information on the use of plants to treat the different types of beauty issues. Higher FIC values (higher when closer to 1) describe the well-defined selection criterion, and/or information is exchanged between informants. If informants do not exchange information about plants’ uses, FIC values will be near to zero [21].FIC was calculated as follows:(3)FIC=nur−ntnur−1,where “nur” depicts the number of use citations in each category while “nt” indicates the number of used taxa.


## 2.4.4. Relative Importance (RI)

The following formula was used to calculate the RI:(4)RI=NUC+NT,where NUC = number of use categories of a given species/total number of use categories of the most versatile species. NT = types of uses attributed to a given species/total number of types of uses attributed to the most important taxon.


## 2.4.5. Statistical Analysis

Descriptive statistics were used to present the collected data of the survey. Correlation analysis based on simple linear regression between RFC and UV, RFC and RI, and UV and RI was performed. Statistical significance was set at 5%. The programmes used were Microsoft Excel 2016 and Minitab 17.


## 3. Results and Discussion

### 3.1. General Analysis

#### 3.1.1. Demographic Information of the Informants

A total 120 of informants were interviewed and categorized into different demographic categories as listed in Table3. There were 43.33% male informants and 56.67% female informants. Most informants were in the 41–50 age group followed by 51–60, 30–40, and 60 < age groups. It was reported as 52.5%, 26.67%, 14.17%, and 6.67%, respectively. On the basis on experience, 37.5% of informants had more than 20 years’ experience while 10.00% of informants had less than 5 years’ experience. And 30.83% and 21.67% of informants had the experience between 11–20 and 5–10 years, respectively. 50.83% of informants had a bachelor’s degree whereas 24.17% had a bachelor’s degree with postgraduate education. Furthermore, 25.00% of informants had the inherited knowledge from their families which is known as indigenous knowledge.Table 3
Demographic information about informants.
ParameterPercentage (%)GenderMale43.33Female56.67Age group (years)30–4014.1741–5052.551–6026.6760<6.67Experience (years)<510.005–1021.6711–2030.8320<37.5EducationInherited from family (indigenous)25.00Bachelor50.83Bachelor + postgraduate24.17


#### 3.1.2. Plant Families

A total of 133 different plant species belonging to 64 families were utilized in cosmetic treatments. Of these, 72 plant species are currently used in commercialized herbal cosmetic products in Sri Lanka [22]. The most dominant family was reported as family Fabaceae (21 species, 16%). This was followed by Rutaceae (8 species, 6%); Malvaceae and Zingiberaceae (6 species per each, 5%); Rubiaceae (5 species, 4%); Convolvulaceae, Lamiaceae, and Myrtaceae (4 species per each, 3%); Acanthaceae, Apocynaceae, Arecaceae, Combretaceae, Cucurbitaceae, and Lecythidaceae (3 species per each, 2%); Asteraceae, Celastraceae, Clusiaceae, Oleaceae, Poaceae, Sapindaceae, and Solanaceae (2 species per each, 2%); and one species each for the rest of the families. Table 2 lists the medicinal plants used in cosmetic treatments by traditional and Ayurveda practitioners of Sri Lanka. Moreover, the greatest utilization of medicinal plants in family Fabaceae under different disciplines such as Ayurveda, traditional systems of medicine, and medicinal plant-related industries in Sri Lanka has also been reported by Napagoda et al. [4], Nirmalan [9], Kankanamalage et al. [23], and Dharmadasa et al. [24].


#### 3.1.3. Different Plant Parts Used in Cosmetic Treatments

As shown in Figure2, a wide range of plant parts were used for cosmetic treatments in Sri Lanka. Identified plant parts were described as leaves, bark, seeds, fruits, roots, flowers, rhizome, stem, heartwood, flower buds, tuber, gum, fruit rind, shoots, bulb, flower stamens, fruit kernel, inner bark, leaf gel, pods, thorns, and wood. The most utilized plant part was stated as leaves (34%). This was followed by bark (14%), seeds (12%), fruit (11%), roots (8%), flowers (7%), rhizome (5%), stem (4%), heartwood (3%), flower buds (2%), tuber, gum, fruit rind, shoots (2% per each), bulb, flower stamens, fruit kernel, inner bark, leaf gel, pods, thorn, and wood (1% per each), respectively. However, usage of entire plant was recorded as 6%. In line with the studies of Napagoda et al. [4], Kumarasinghe [7], Nirmalan [9], Kankanamalage et al. [23], and Dharmadasa et al. [24], leaves were the commonest part of plants used in different treatments of Ayurveda, traditional systems of medicine, and medicinal plant-based industries in Sri Lanka. Moreover, Kumarasinghe, [7] stated that availability of most effective ingredients in leaves results in the highest usage. However, more scientific studies related to this area are required. As other reasons, Dharmadasa et al. [24] highlight availability in large quantities, easy accessibility, and cheaper cost for the abundance usage of leaves. These are further agreement with scientific studies conducted by Miraldi et al. [25], Ghorbani et al. [26] and Mowobi et al. [27] from other countries.Figure 2
Different parts of the plants used in cosmetic treatments of Sri Lanka.


#### 3.1.4. Plants Used in Different Cosmetic Treatments

Plant species used for cosmetic treatments were classified into three main categories called skin care, hair care, and oral care. Out of 133 plants, the highest numbers of plants were used in skin care treatments. As shown in Figure3, it was reported as 52 plant species (39%). 26 plant species (20%) and 23 plant species (17%) were used for hair care and oral care treatments, respectively. Moreover, usages of 18 plant species (14%) were reported in both skin and hair care treatments while usages of 12 plant species (9%) were recorded in both skin and oral care treatments. However, the usage of Myristica fragrans Houtt. and Kaempferia galanga L. was stated in all three cosmetic treatments. Furthermore, research study conducted by Nirmalan [9] on cosmetic perspectives of ethnobotany in Northern part of Sri Lanka confirms the higher usage of local plants for skin care followed by hair care.Figure 3
Plant usage in different cosmetic treatments.According to the market research report on “Organic Personal Care and Cosmetic Products Market by Product Type (Skin Care, Hair Care, Oral Care, and Makeup Cosmetics) and by Distribution Channel (Retail Sale and Online Sale), Global Opportunity Analysis and Industry Forecast, 2015–2022” [28], natural and organic skin care product category is the top billing in the global organic beauty market, and it is expected to emerge as the most attractive segment with 30.9% share by 2024, followed by hair care. Furthermore, research report on “Herbal Beauty Market: Global Industry Analysis and Opportunity Assessment 2015–2025” [29], reports North America and Europe hold the largest market share for herbal sun care and herbal skin care products at present.


#### 3.1.5. Different Mode of Applications in Cosmetic Treatments

Medicinal plants used in folk herbal remedies were prepared and administered in various forms. Results revealed that there were 08 types of different applications in cosmetic treatments (Figure4). These include paste, juice, oil, decoction, powder, infusion, gel, and salad. Number of plants used in each application was reported as 49 plant species (37%) as paste, 21 plant species (16%) as juice, 19 plant species (14%) as oil, 16 plant species (12%) as decoction, 8 plant species (6%) as powder, 10 plant species (8%) as infusion, and 01 plant species per each (1%) as gel and salad. However, 10 plant species were reported without specific mode of application while 12 plants were recorded in usage as fragrant agents. Therefore, these 22 plants were included under “others” category. The majority of herbal recipes were prepared using water as the medium. However, honey, coconut oil, and sesame oil were the most commonly used adjuvants in cosmetic treatments. For example, oil prepared by boiling leaves and flowers of Hibiscus rosa-sinensis L. with coconut oil or sesame oil is popular application for promoting healthy hair growth, preventing early grey hair, hair fall, and dandruff. Furthermore, Cinnamomum verum J.Presl powder mixed with honey is commonly used to treat pimples.Figure 4
Different modes of applications.


### 3.2. Quantitative Analysis of Data

#### 3.2.1. Relative Frequency of Citation (RFC)

Relative frequency of citation was calculated to identify the most common occurring medicinal plants used for topical cosmetic treatments. Based on RFC values,Aloe vera (L.) Burm.f. reported the highest RFC value (0.83) while Santalum album L. Coscinium fenestratum (Goetgh.) Colebr. and Azadirachta indica A.Juss. showed the second highest RFC value (0.74). In addition, Kokoona zeylanica, Tephrosia purpurea (L.) Pers., Theobroma cacao L., and Psidium guajava L. signified 0.73 RFC value. Terminalia bellirica (Gaertn.) Roxb., Elaeocarpus serratus L., Cymbopogon citratus (DC.) Stapf, Centella asiatica (L.) Urb, Terminalia chebula Retz., Sesamum indicum L., Curcuma longa L., Vetiveria zizanioides (L) Nash, Myristica fragrans Houtt., Mimusops elengi L., Citrus limon (L.) Osbeck, Eclipta prostrata (L.)L, Pterocarpus santalinus L.f, Curcuma aromatica Salisb., Hemidesmus indicus (L.) R. Br. ex Schult., Cucumis sativus L., Dillenia retusa Thunb., Trigonella foenum-graecum L., Cocos nucifera L., Acorus calamus L., Carica papaya L., Asparagus racemosus Willd., Indigofera tinctoria L., and Cinnamomum verum J. Presl were other highly cited medicinal plants by traditional practitioners and Ayurveda physicians. The higher RFC values of medicinal plants depict that these plants are well known to the maximum number of informants in terms of cosmetic treatments [19]. However, the least RFC value was reported as 0.01 and it was represented by 24 medicinal plants. Those were Vernonia anthelmintica (L.) Willd., Cuscuta chinensis Lam., Costus speciosus (Koenig) Smith, Cucumis melo L., Diospyros malabarica (Desr.) Kostel., Acacia concinna (Willd.) DC., Acacia nilotica (L.) Delile, Crotalaria verrucosa L., Pterocarpus marsupium Roxb., Saraca asoca (Roxb.) Willd., Curculigo orchioides Gaertn., Pogostemon heyneanus Benth., Premna obtusifolia R.Br., Tectona grandis L.f., Barringtonia acutangula (L.) Gaertn., Careya arborea Roxb., Couroupita guianensis Aubl., Abutilon indicum (L.) Sweet, Geophila repens (L.) I.M.Johnst., Oldenlandia corymbosa L., Datura metel L., Symplocos cochinchinensis (Lour.) S. Moore, Leea indica (Burm.f.) Merr., and Alpinia malaccensis (Burm.f.) Roscoe. Unfortunately, other ethnobotanical studies conducted in Sri Lanka have not focused to this index. Therefore, it was not possible to compare data.


#### 3.2.2. Use Value (UV)

This index highlights the connotation between the medicinal plant species and uses allocated to it [19]. Based on the results of the study, UV was greater than 01 in 19 medicinal plant species while 18 medicinal plants reported the least UV value. The highest UV value was recorded from the Aloe vera (L.) Burm.f. It was reported as 3.66. This was followed by Asparagus racemosus Willd. (2.20), Santalum album L. (1.91), Myristica fragrans Houtt. (1.53), Kokoona zeylanica Thwaites (1.37), Indigofera tinctoria L. (1.30), Citrus aurantifolia Panzer. (Christm.) Swingle (1.30), Centella asiatica (L.) Urb (1.27), Coscinium fenestratum (Goetgh.) Colebr. (1.27), Azadirachta indica A.Juss (1.22), Cymbopogon citratus (DC.) Stapf (1.13), Terminalia bellirica (Gaertn.) Roxb. (1.12), Sesamum indicum L. (1.12), Citrus limon (L.) Osbeck (1.10), Eclipta prostrata (L.) L (1.09), Theobroma cacao L. (1.09), Acorus calamus L. (1.05), Terminalia chebula Retz. (1.03), and Dillenia retusa Thunb. (1.00). High UVs indicate the frequent usage of these medicinal plant materials for cosmetic treatments by traditional practitioners and Ayurveda physicians with high use reports. Although the UV index was not calculated, ethnobotanical surveys conducted to find out medicinal plants used in anti-inflammatory remedies [4], skin diseases [7], and snake bites treatments [24] of Sri Lanka revealed about greater usage of Azadirachta indica A.Juss., in skin diseases treatments, Citrus aurantifolia (Christm.) Swingle in snake bites treatments, and Coscinium fenestratum (Goetgh.) Colebr. in anti-inflammatory remedies, respectively. According to the survey on medicinal materials used in traditional systems of medicine in Sri Lanka, Aloe vera (L.) Burm.f., Santalum album L., Coscinium fenestratum (Goetgh.) Colebr., and Acorus calamus L. have been listed as heavily used medicinal plants in Sri Lanka. Moreover, Sesamum indicum L., Azadirachta indica A.Juss, Indigofera tinctoria L., Terminalia bellirica (Gaertn.) Roxb., Terminalia chebula Retz., Asparagus racemosus Willd., and Centella asiatica (L.) Urb have been listed as the most demanded plant materials which show heavy usage in the Sri Lankan traditional systems of medicine [23].


#### 3.2.3. Relative Importance (RI)

There were 12 medicinal plants which recorded the RI value greater than 1. The highest RI value was reported fromAsparagus racemosus Willd. and Hibiscus rosa-sinensis L. It was 1.67. This was followed by Kaempferia galanga L. (1.57), Aloe vera (L.) Burm.f. (1.52), Myristica fragrans Houtt. (1.43), Citrus aurantifolia Panzer. (Christm.) Swingle (1.24), Acorus calamus L. (1.10), Eclipta prostrata (L.) L (1.10), Indigofera tinctoria L. (1.10), Jasminum grandiflorum L. (1.10), Phyllanthus emblica L. (1.10), and Morinda citrifolia L. (1.10). The lowest RI value was 0.48, and it was recorded from 73 medicinal plants. This may possibly be due to the less used categories and a few used attributes of these plants.


#### 3.2.4. Factor Informant Consensus (FIC)

The results of the FIC (Table4) showed that treatments for wrinkles and foot health improvements (1.00) under the skin care category, treatments for damaged hair and scalp cooling (1.00) under the hair care category, and treatments for malodour of the mouth (0.96) under oral care category had the higher degree of consensus. All these values were comparatively high, thus indicating good agreement among traditional practitioners and Ayurveda physicians on the knowledge and consequent use of particular plants or plant materials to treat different beauty issues.Table 4
Factor informant consensus (FIC) for different beauty issues.
CategoryTreatments forNumber of plantsCitationsFICSkin careImproving skin complexion25630.61Pimples23800.72Freckles13730.83Skin discoloration9640.87Healing8780.91Aging7720.92Acne4640.95Skin dryness4820.96Exfoliating4180.82Soften the skin4430.93Malodour of the body2290.96Cleansing2820.99Improving skin health290.88Whitening2300.97Wrinkles1161.00Improving foot health121.00Hair careHealthy hair20880.78Dandruff11770.87Pediculosis9830.90Scalp and hair cleansing8870.92Hair fallen8720.90Grey hair6520.90Improving hair color4870.97Damaged hair1861.00Scalp cooling1221.00Oral careImproving oral hygiene34880.62Malodour of the mouth3480.96Medicinal plants are used in cosmetic treatments for various reasons. The close analysis of the survey revealed the medicinal plant usage in 16 skin-related issues, 9 hair-related issues, and 2 major oral-related issues. The majority of medicinal plants used in skin care treatments were used to improve skin complexion. It was reported as 25 medicinal plants. Treatment for pimples was the second largest treatment segment under the skin care which reported 23 medicinal plants in usage. Other than these main two treatments, herbal preparations were used to treat for freckles (13 plants), skin discoloration (9 plants), healing (8 plants), aging (7 plants) and acne, skin dryness, exfoliating and soften the skin (4 plants per each), malodour of the body, cleansing, skin health, and whitening (2 plants per each).Camellia sinensis (L.) Kuntze is used to treat for skin wrinkles. Furthermore, Ipomoea pes-caprae (L.) R. Br. is utilized to improve foot health. The most of hair care treatments were done for improving the healthy hair followed by dandruff treatments. It was reported as usage of 20 and 11 medicinal plants, respectively. Moreover, treatments were recorded for pediculosis (9 plants), cleansing and hair fallen (8 plants per each), grey hair (6 plants), and improving hair color (4 plants). Moreover, Centella asiatica (L.) Urb is used to treat damaged hair whilst Jasminum grandiflorum L. plant extracted oil is used for scalp cooling. Majority of treatments under oral care were reported for improving oral hygiene. Most of treatments of oral care were prepared as mouth wash. 34 medicinal plants used to improve oral hygiene were reported. Furthermore, Cryptolepis dubia (Burm.f.) M.R. Almeida, Saraca asoca (Roxb.) Willd., and Hibiscus abelmoschus L. are used to treat particularly for malodour of the mouth. In addition, 12 medicinal plant species which used as fragrant agent were also recorded.


#### 3.2.5. Correlation Analysis

Correlations among different quantitative measures are indicated in Figure5. RFC was moderately correlated with UV (Figure 5(a), R2 = 0.69, P=0.0001) while high correlation was reported between RI and UV (Figure 5(b), R2 = 0.898, P=0.0001) and RFC and RI (Figure 5(c), R2 = 0.931, P=0.0001), giving good linearity between variables. Therefore, this is proven that identified plant species significantly have the same importance irrespective to the methodology employed.Figure 5
Correlation among different quantitative measures. (a) Correlation between RFC and UV. (b) Correlation between RI and UV. (c) Correlation between RFC and RI.
(a)(b)(c)


## 3.1. General Analysis

### 3.1.1. Demographic Information of the Informants

A total 120 of informants were interviewed and categorized into different demographic categories as listed in Table3. There were 43.33% male informants and 56.67% female informants. Most informants were in the 41–50 age group followed by 51–60, 30–40, and 60 < age groups. It was reported as 52.5%, 26.67%, 14.17%, and 6.67%, respectively. On the basis on experience, 37.5% of informants had more than 20 years’ experience while 10.00% of informants had less than 5 years’ experience. And 30.83% and 21.67% of informants had the experience between 11–20 and 5–10 years, respectively. 50.83% of informants had a bachelor’s degree whereas 24.17% had a bachelor’s degree with postgraduate education. Furthermore, 25.00% of informants had the inherited knowledge from their families which is known as indigenous knowledge.Table 3
Demographic information about informants.
ParameterPercentage (%)GenderMale43.33Female56.67Age group (years)30–4014.1741–5052.551–6026.6760<6.67Experience (years)<510.005–1021.6711–2030.8320<37.5EducationInherited from family (indigenous)25.00Bachelor50.83Bachelor + postgraduate24.17


### 3.1.2. Plant Families

A total of 133 different plant species belonging to 64 families were utilized in cosmetic treatments. Of these, 72 plant species are currently used in commercialized herbal cosmetic products in Sri Lanka [22]. The most dominant family was reported as family Fabaceae (21 species, 16%). This was followed by Rutaceae (8 species, 6%); Malvaceae and Zingiberaceae (6 species per each, 5%); Rubiaceae (5 species, 4%); Convolvulaceae, Lamiaceae, and Myrtaceae (4 species per each, 3%); Acanthaceae, Apocynaceae, Arecaceae, Combretaceae, Cucurbitaceae, and Lecythidaceae (3 species per each, 2%); Asteraceae, Celastraceae, Clusiaceae, Oleaceae, Poaceae, Sapindaceae, and Solanaceae (2 species per each, 2%); and one species each for the rest of the families. Table 2 lists the medicinal plants used in cosmetic treatments by traditional and Ayurveda practitioners of Sri Lanka. Moreover, the greatest utilization of medicinal plants in family Fabaceae under different disciplines such as Ayurveda, traditional systems of medicine, and medicinal plant-related industries in Sri Lanka has also been reported by Napagoda et al. [4], Nirmalan [9], Kankanamalage et al. [23], and Dharmadasa et al. [24].


### 3.1.3. Different Plant Parts Used in Cosmetic Treatments

As shown in Figure2, a wide range of plant parts were used for cosmetic treatments in Sri Lanka. Identified plant parts were described as leaves, bark, seeds, fruits, roots, flowers, rhizome, stem, heartwood, flower buds, tuber, gum, fruit rind, shoots, bulb, flower stamens, fruit kernel, inner bark, leaf gel, pods, thorns, and wood. The most utilized plant part was stated as leaves (34%). This was followed by bark (14%), seeds (12%), fruit (11%), roots (8%), flowers (7%), rhizome (5%), stem (4%), heartwood (3%), flower buds (2%), tuber, gum, fruit rind, shoots (2% per each), bulb, flower stamens, fruit kernel, inner bark, leaf gel, pods, thorn, and wood (1% per each), respectively. However, usage of entire plant was recorded as 6%. In line with the studies of Napagoda et al. [4], Kumarasinghe [7], Nirmalan [9], Kankanamalage et al. [23], and Dharmadasa et al. [24], leaves were the commonest part of plants used in different treatments of Ayurveda, traditional systems of medicine, and medicinal plant-based industries in Sri Lanka. Moreover, Kumarasinghe, [7] stated that availability of most effective ingredients in leaves results in the highest usage. However, more scientific studies related to this area are required. As other reasons, Dharmadasa et al. [24] highlight availability in large quantities, easy accessibility, and cheaper cost for the abundance usage of leaves. These are further agreement with scientific studies conducted by Miraldi et al. [25], Ghorbani et al. [26] and Mowobi et al. [27] from other countries.Figure 2
Different parts of the plants used in cosmetic treatments of Sri Lanka.


### 3.1.4. Plants Used in Different Cosmetic Treatments

Plant species used for cosmetic treatments were classified into three main categories called skin care, hair care, and oral care. Out of 133 plants, the highest numbers of plants were used in skin care treatments. As shown in Figure3, it was reported as 52 plant species (39%). 26 plant species (20%) and 23 plant species (17%) were used for hair care and oral care treatments, respectively. Moreover, usages of 18 plant species (14%) were reported in both skin and hair care treatments while usages of 12 plant species (9%) were recorded in both skin and oral care treatments. However, the usage of Myristica fragrans Houtt. and Kaempferia galanga L. was stated in all three cosmetic treatments. Furthermore, research study conducted by Nirmalan [9] on cosmetic perspectives of ethnobotany in Northern part of Sri Lanka confirms the higher usage of local plants for skin care followed by hair care.Figure 3
Plant usage in different cosmetic treatments.According to the market research report on “Organic Personal Care and Cosmetic Products Market by Product Type (Skin Care, Hair Care, Oral Care, and Makeup Cosmetics) and by Distribution Channel (Retail Sale and Online Sale), Global Opportunity Analysis and Industry Forecast, 2015–2022” [28], natural and organic skin care product category is the top billing in the global organic beauty market, and it is expected to emerge as the most attractive segment with 30.9% share by 2024, followed by hair care. Furthermore, research report on “Herbal Beauty Market: Global Industry Analysis and Opportunity Assessment 2015–2025” [29], reports North America and Europe hold the largest market share for herbal sun care and herbal skin care products at present.


### 3.1.5. Different Mode of Applications in Cosmetic Treatments

Medicinal plants used in folk herbal remedies were prepared and administered in various forms. Results revealed that there were 08 types of different applications in cosmetic treatments (Figure4). These include paste, juice, oil, decoction, powder, infusion, gel, and salad. Number of plants used in each application was reported as 49 plant species (37%) as paste, 21 plant species (16%) as juice, 19 plant species (14%) as oil, 16 plant species (12%) as decoction, 8 plant species (6%) as powder, 10 plant species (8%) as infusion, and 01 plant species per each (1%) as gel and salad. However, 10 plant species were reported without specific mode of application while 12 plants were recorded in usage as fragrant agents. Therefore, these 22 plants were included under “others” category. The majority of herbal recipes were prepared using water as the medium. However, honey, coconut oil, and sesame oil were the most commonly used adjuvants in cosmetic treatments. For example, oil prepared by boiling leaves and flowers of Hibiscus rosa-sinensis L. with coconut oil or sesame oil is popular application for promoting healthy hair growth, preventing early grey hair, hair fall, and dandruff. Furthermore, Cinnamomum verum J.Presl powder mixed with honey is commonly used to treat pimples.Figure 4
Different modes of applications.


## 3.1.1. Demographic Information of the Informants

A total 120 of informants were interviewed and categorized into different demographic categories as listed in Table3. There were 43.33% male informants and 56.67% female informants. Most informants were in the 41–50 age group followed by 51–60, 30–40, and 60 < age groups. It was reported as 52.5%, 26.67%, 14.17%, and 6.67%, respectively. On the basis on experience, 37.5% of informants had more than 20 years’ experience while 10.00% of informants had less than 5 years’ experience. And 30.83% and 21.67% of informants had the experience between 11–20 and 5–10 years, respectively. 50.83% of informants had a bachelor’s degree whereas 24.17% had a bachelor’s degree with postgraduate education. Furthermore, 25.00% of informants had the inherited knowledge from their families which is known as indigenous knowledge.Table 3
Demographic information about informants.
ParameterPercentage (%)GenderMale43.33Female56.67Age group (years)30–4014.1741–5052.551–6026.6760<6.67Experience (years)<510.005–1021.6711–2030.8320<37.5EducationInherited from family (indigenous)25.00Bachelor50.83Bachelor + postgraduate24.17


## 3.1.2. Plant Families

A total of 133 different plant species belonging to 64 families were utilized in cosmetic treatments. Of these, 72 plant species are currently used in commercialized herbal cosmetic products in Sri Lanka [22]. The most dominant family was reported as family Fabaceae (21 species, 16%). This was followed by Rutaceae (8 species, 6%); Malvaceae and Zingiberaceae (6 species per each, 5%); Rubiaceae (5 species, 4%); Convolvulaceae, Lamiaceae, and Myrtaceae (4 species per each, 3%); Acanthaceae, Apocynaceae, Arecaceae, Combretaceae, Cucurbitaceae, and Lecythidaceae (3 species per each, 2%); Asteraceae, Celastraceae, Clusiaceae, Oleaceae, Poaceae, Sapindaceae, and Solanaceae (2 species per each, 2%); and one species each for the rest of the families. Table 2 lists the medicinal plants used in cosmetic treatments by traditional and Ayurveda practitioners of Sri Lanka. Moreover, the greatest utilization of medicinal plants in family Fabaceae under different disciplines such as Ayurveda, traditional systems of medicine, and medicinal plant-related industries in Sri Lanka has also been reported by Napagoda et al. [4], Nirmalan [9], Kankanamalage et al. [23], and Dharmadasa et al. [24].


## 3.1.3. Different Plant Parts Used in Cosmetic Treatments

As shown in Figure2, a wide range of plant parts were used for cosmetic treatments in Sri Lanka. Identified plant parts were described as leaves, bark, seeds, fruits, roots, flowers, rhizome, stem, heartwood, flower buds, tuber, gum, fruit rind, shoots, bulb, flower stamens, fruit kernel, inner bark, leaf gel, pods, thorns, and wood. The most utilized plant part was stated as leaves (34%). This was followed by bark (14%), seeds (12%), fruit (11%), roots (8%), flowers (7%), rhizome (5%), stem (4%), heartwood (3%), flower buds (2%), tuber, gum, fruit rind, shoots (2% per each), bulb, flower stamens, fruit kernel, inner bark, leaf gel, pods, thorn, and wood (1% per each), respectively. However, usage of entire plant was recorded as 6%. In line with the studies of Napagoda et al. [4], Kumarasinghe [7], Nirmalan [9], Kankanamalage et al. [23], and Dharmadasa et al. [24], leaves were the commonest part of plants used in different treatments of Ayurveda, traditional systems of medicine, and medicinal plant-based industries in Sri Lanka. Moreover, Kumarasinghe, [7] stated that availability of most effective ingredients in leaves results in the highest usage. However, more scientific studies related to this area are required. As other reasons, Dharmadasa et al. [24] highlight availability in large quantities, easy accessibility, and cheaper cost for the abundance usage of leaves. These are further agreement with scientific studies conducted by Miraldi et al. [25], Ghorbani et al. [26] and Mowobi et al. [27] from other countries.Figure 2
Different parts of the plants used in cosmetic treatments of Sri Lanka.


## 3.1.4. Plants Used in Different Cosmetic Treatments

Plant species used for cosmetic treatments were classified into three main categories called skin care, hair care, and oral care. Out of 133 plants, the highest numbers of plants were used in skin care treatments. As shown in Figure3, it was reported as 52 plant species (39%). 26 plant species (20%) and 23 plant species (17%) were used for hair care and oral care treatments, respectively. Moreover, usages of 18 plant species (14%) were reported in both skin and hair care treatments while usages of 12 plant species (9%) were recorded in both skin and oral care treatments. However, the usage of Myristica fragrans Houtt. and Kaempferia galanga L. was stated in all three cosmetic treatments. Furthermore, research study conducted by Nirmalan [9] on cosmetic perspectives of ethnobotany in Northern part of Sri Lanka confirms the higher usage of local plants for skin care followed by hair care.Figure 3
Plant usage in different cosmetic treatments.According to the market research report on “Organic Personal Care and Cosmetic Products Market by Product Type (Skin Care, Hair Care, Oral Care, and Makeup Cosmetics) and by Distribution Channel (Retail Sale and Online Sale), Global Opportunity Analysis and Industry Forecast, 2015–2022” [28], natural and organic skin care product category is the top billing in the global organic beauty market, and it is expected to emerge as the most attractive segment with 30.9% share by 2024, followed by hair care. Furthermore, research report on “Herbal Beauty Market: Global Industry Analysis and Opportunity Assessment 2015–2025” [29], reports North America and Europe hold the largest market share for herbal sun care and herbal skin care products at present.


## 3.1.5. Different Mode of Applications in Cosmetic Treatments

Medicinal plants used in folk herbal remedies were prepared and administered in various forms. Results revealed that there were 08 types of different applications in cosmetic treatments (Figure4). These include paste, juice, oil, decoction, powder, infusion, gel, and salad. Number of plants used in each application was reported as 49 plant species (37%) as paste, 21 plant species (16%) as juice, 19 plant species (14%) as oil, 16 plant species (12%) as decoction, 8 plant species (6%) as powder, 10 plant species (8%) as infusion, and 01 plant species per each (1%) as gel and salad. However, 10 plant species were reported without specific mode of application while 12 plants were recorded in usage as fragrant agents. Therefore, these 22 plants were included under “others” category. The majority of herbal recipes were prepared using water as the medium. However, honey, coconut oil, and sesame oil were the most commonly used adjuvants in cosmetic treatments. For example, oil prepared by boiling leaves and flowers of Hibiscus rosa-sinensis L. with coconut oil or sesame oil is popular application for promoting healthy hair growth, preventing early grey hair, hair fall, and dandruff. Furthermore, Cinnamomum verum J.Presl powder mixed with honey is commonly used to treat pimples.Figure 4
Different modes of applications.


## 3.2. Quantitative Analysis of Data

### 3.2.1. Relative Frequency of Citation (RFC)

Relative frequency of citation was calculated to identify the most common occurring medicinal plants used for topical cosmetic treatments. Based on RFC values,Aloe vera (L.) Burm.f. reported the highest RFC value (0.83) while Santalum album L. Coscinium fenestratum (Goetgh.) Colebr. and Azadirachta indica A.Juss. showed the second highest RFC value (0.74). In addition, Kokoona zeylanica, Tephrosia purpurea (L.) Pers., Theobroma cacao L., and Psidium guajava L. signified 0.73 RFC value. Terminalia bellirica (Gaertn.) Roxb., Elaeocarpus serratus L., Cymbopogon citratus (DC.) Stapf, Centella asiatica (L.) Urb, Terminalia chebula Retz., Sesamum indicum L., Curcuma longa L., Vetiveria zizanioides (L) Nash, Myristica fragrans Houtt., Mimusops elengi L., Citrus limon (L.) Osbeck, Eclipta prostrata (L.)L, Pterocarpus santalinus L.f, Curcuma aromatica Salisb., Hemidesmus indicus (L.) R. Br. ex Schult., Cucumis sativus L., Dillenia retusa Thunb., Trigonella foenum-graecum L., Cocos nucifera L., Acorus calamus L., Carica papaya L., Asparagus racemosus Willd., Indigofera tinctoria L., and Cinnamomum verum J. Presl were other highly cited medicinal plants by traditional practitioners and Ayurveda physicians. The higher RFC values of medicinal plants depict that these plants are well known to the maximum number of informants in terms of cosmetic treatments [19]. However, the least RFC value was reported as 0.01 and it was represented by 24 medicinal plants. Those were Vernonia anthelmintica (L.) Willd., Cuscuta chinensis Lam., Costus speciosus (Koenig) Smith, Cucumis melo L., Diospyros malabarica (Desr.) Kostel., Acacia concinna (Willd.) DC., Acacia nilotica (L.) Delile, Crotalaria verrucosa L., Pterocarpus marsupium Roxb., Saraca asoca (Roxb.) Willd., Curculigo orchioides Gaertn., Pogostemon heyneanus Benth., Premna obtusifolia R.Br., Tectona grandis L.f., Barringtonia acutangula (L.) Gaertn., Careya arborea Roxb., Couroupita guianensis Aubl., Abutilon indicum (L.) Sweet, Geophila repens (L.) I.M.Johnst., Oldenlandia corymbosa L., Datura metel L., Symplocos cochinchinensis (Lour.) S. Moore, Leea indica (Burm.f.) Merr., and Alpinia malaccensis (Burm.f.) Roscoe. Unfortunately, other ethnobotanical studies conducted in Sri Lanka have not focused to this index. Therefore, it was not possible to compare data.


### 3.2.2. Use Value (UV)

This index highlights the connotation between the medicinal plant species and uses allocated to it [19]. Based on the results of the study, UV was greater than 01 in 19 medicinal plant species while 18 medicinal plants reported the least UV value. The highest UV value was recorded from the Aloe vera (L.) Burm.f. It was reported as 3.66. This was followed by Asparagus racemosus Willd. (2.20), Santalum album L. (1.91), Myristica fragrans Houtt. (1.53), Kokoona zeylanica Thwaites (1.37), Indigofera tinctoria L. (1.30), Citrus aurantifolia Panzer. (Christm.) Swingle (1.30), Centella asiatica (L.) Urb (1.27), Coscinium fenestratum (Goetgh.) Colebr. (1.27), Azadirachta indica A.Juss (1.22), Cymbopogon citratus (DC.) Stapf (1.13), Terminalia bellirica (Gaertn.) Roxb. (1.12), Sesamum indicum L. (1.12), Citrus limon (L.) Osbeck (1.10), Eclipta prostrata (L.) L (1.09), Theobroma cacao L. (1.09), Acorus calamus L. (1.05), Terminalia chebula Retz. (1.03), and Dillenia retusa Thunb. (1.00). High UVs indicate the frequent usage of these medicinal plant materials for cosmetic treatments by traditional practitioners and Ayurveda physicians with high use reports. Although the UV index was not calculated, ethnobotanical surveys conducted to find out medicinal plants used in anti-inflammatory remedies [4], skin diseases [7], and snake bites treatments [24] of Sri Lanka revealed about greater usage of Azadirachta indica A.Juss., in skin diseases treatments, Citrus aurantifolia (Christm.) Swingle in snake bites treatments, and Coscinium fenestratum (Goetgh.) Colebr. in anti-inflammatory remedies, respectively. According to the survey on medicinal materials used in traditional systems of medicine in Sri Lanka, Aloe vera (L.) Burm.f., Santalum album L., Coscinium fenestratum (Goetgh.) Colebr., and Acorus calamus L. have been listed as heavily used medicinal plants in Sri Lanka. Moreover, Sesamum indicum L., Azadirachta indica A.Juss, Indigofera tinctoria L., Terminalia bellirica (Gaertn.) Roxb., Terminalia chebula Retz., Asparagus racemosus Willd., and Centella asiatica (L.) Urb have been listed as the most demanded plant materials which show heavy usage in the Sri Lankan traditional systems of medicine [23].


### 3.2.3. Relative Importance (RI)

There were 12 medicinal plants which recorded the RI value greater than 1. The highest RI value was reported fromAsparagus racemosus Willd. and Hibiscus rosa-sinensis L. It was 1.67. This was followed by Kaempferia galanga L. (1.57), Aloe vera (L.) Burm.f. (1.52), Myristica fragrans Houtt. (1.43), Citrus aurantifolia Panzer. (Christm.) Swingle (1.24), Acorus calamus L. (1.10), Eclipta prostrata (L.) L (1.10), Indigofera tinctoria L. (1.10), Jasminum grandiflorum L. (1.10), Phyllanthus emblica L. (1.10), and Morinda citrifolia L. (1.10). The lowest RI value was 0.48, and it was recorded from 73 medicinal plants. This may possibly be due to the less used categories and a few used attributes of these plants.


### 3.2.4. Factor Informant Consensus (FIC)

The results of the FIC (Table4) showed that treatments for wrinkles and foot health improvements (1.00) under the skin care category, treatments for damaged hair and scalp cooling (1.00) under the hair care category, and treatments for malodour of the mouth (0.96) under oral care category had the higher degree of consensus. All these values were comparatively high, thus indicating good agreement among traditional practitioners and Ayurveda physicians on the knowledge and consequent use of particular plants or plant materials to treat different beauty issues.Table 4
Factor informant consensus (FIC) for different beauty issues.
CategoryTreatments forNumber of plantsCitationsFICSkin careImproving skin complexion25630.61Pimples23800.72Freckles13730.83Skin discoloration9640.87Healing8780.91Aging7720.92Acne4640.95Skin dryness4820.96Exfoliating4180.82Soften the skin4430.93Malodour of the body2290.96Cleansing2820.99Improving skin health290.88Whitening2300.97Wrinkles1161.00Improving foot health121.00Hair careHealthy hair20880.78Dandruff11770.87Pediculosis9830.90Scalp and hair cleansing8870.92Hair fallen8720.90Grey hair6520.90Improving hair color4870.97Damaged hair1861.00Scalp cooling1221.00Oral careImproving oral hygiene34880.62Malodour of the mouth3480.96Medicinal plants are used in cosmetic treatments for various reasons. The close analysis of the survey revealed the medicinal plant usage in 16 skin-related issues, 9 hair-related issues, and 2 major oral-related issues. The majority of medicinal plants used in skin care treatments were used to improve skin complexion. It was reported as 25 medicinal plants. Treatment for pimples was the second largest treatment segment under the skin care which reported 23 medicinal plants in usage. Other than these main two treatments, herbal preparations were used to treat for freckles (13 plants), skin discoloration (9 plants), healing (8 plants), aging (7 plants) and acne, skin dryness, exfoliating and soften the skin (4 plants per each), malodour of the body, cleansing, skin health, and whitening (2 plants per each).Camellia sinensis (L.) Kuntze is used to treat for skin wrinkles. Furthermore, Ipomoea pes-caprae (L.) R. Br. is utilized to improve foot health. The most of hair care treatments were done for improving the healthy hair followed by dandruff treatments. It was reported as usage of 20 and 11 medicinal plants, respectively. Moreover, treatments were recorded for pediculosis (9 plants), cleansing and hair fallen (8 plants per each), grey hair (6 plants), and improving hair color (4 plants). Moreover, Centella asiatica (L.) Urb is used to treat damaged hair whilst Jasminum grandiflorum L. plant extracted oil is used for scalp cooling. Majority of treatments under oral care were reported for improving oral hygiene. Most of treatments of oral care were prepared as mouth wash. 34 medicinal plants used to improve oral hygiene were reported. Furthermore, Cryptolepis dubia (Burm.f.) M.R. Almeida, Saraca asoca (Roxb.) Willd., and Hibiscus abelmoschus L. are used to treat particularly for malodour of the mouth. In addition, 12 medicinal plant species which used as fragrant agent were also recorded.


### 3.2.5. Correlation Analysis

Correlations among different quantitative measures are indicated in Figure5. RFC was moderately correlated with UV (Figure 5(a), R2 = 0.69, P=0.0001) while high correlation was reported between RI and UV (Figure 5(b), R2 = 0.898, P=0.0001) and RFC and RI (Figure 5(c), R2 = 0.931, P=0.0001), giving good linearity between variables. Therefore, this is proven that identified plant species significantly have the same importance irrespective to the methodology employed.Figure 5
Correlation among different quantitative measures. (a) Correlation between RFC and UV. (b) Correlation between RI and UV. (c) Correlation between RFC and RI.
(a)(b)(c)


## 3.2.1. Relative Frequency of Citation (RFC)

Relative frequency of citation was calculated to identify the most common occurring medicinal plants used for topical cosmetic treatments. Based on RFC values,Aloe vera (L.) Burm.f. reported the highest RFC value (0.83) while Santalum album L. Coscinium fenestratum (Goetgh.) Colebr. and Azadirachta indica A.Juss. showed the second highest RFC value (0.74). In addition, Kokoona zeylanica, Tephrosia purpurea (L.) Pers., Theobroma cacao L., and Psidium guajava L. signified 0.73 RFC value. Terminalia bellirica (Gaertn.) Roxb., Elaeocarpus serratus L., Cymbopogon citratus (DC.) Stapf, Centella asiatica (L.) Urb, Terminalia chebula Retz., Sesamum indicum L., Curcuma longa L., Vetiveria zizanioides (L) Nash, Myristica fragrans Houtt., Mimusops elengi L., Citrus limon (L.) Osbeck, Eclipta prostrata (L.)L, Pterocarpus santalinus L.f, Curcuma aromatica Salisb., Hemidesmus indicus (L.) R. Br. ex Schult., Cucumis sativus L., Dillenia retusa Thunb., Trigonella foenum-graecum L., Cocos nucifera L., Acorus calamus L., Carica papaya L., Asparagus racemosus Willd., Indigofera tinctoria L., and Cinnamomum verum J. Presl were other highly cited medicinal plants by traditional practitioners and Ayurveda physicians. The higher RFC values of medicinal plants depict that these plants are well known to the maximum number of informants in terms of cosmetic treatments [19]. However, the least RFC value was reported as 0.01 and it was represented by 24 medicinal plants. Those were Vernonia anthelmintica (L.) Willd., Cuscuta chinensis Lam., Costus speciosus (Koenig) Smith, Cucumis melo L., Diospyros malabarica (Desr.) Kostel., Acacia concinna (Willd.) DC., Acacia nilotica (L.) Delile, Crotalaria verrucosa L., Pterocarpus marsupium Roxb., Saraca asoca (Roxb.) Willd., Curculigo orchioides Gaertn., Pogostemon heyneanus Benth., Premna obtusifolia R.Br., Tectona grandis L.f., Barringtonia acutangula (L.) Gaertn., Careya arborea Roxb., Couroupita guianensis Aubl., Abutilon indicum (L.) Sweet, Geophila repens (L.) I.M.Johnst., Oldenlandia corymbosa L., Datura metel L., Symplocos cochinchinensis (Lour.) S. Moore, Leea indica (Burm.f.) Merr., and Alpinia malaccensis (Burm.f.) Roscoe. Unfortunately, other ethnobotanical studies conducted in Sri Lanka have not focused to this index. Therefore, it was not possible to compare data.


## 3.2.2. Use Value (UV)

This index highlights the connotation between the medicinal plant species and uses allocated to it [19]. Based on the results of the study, UV was greater than 01 in 19 medicinal plant species while 18 medicinal plants reported the least UV value. The highest UV value was recorded from the Aloe vera (L.) Burm.f. It was reported as 3.66. This was followed by Asparagus racemosus Willd. (2.20), Santalum album L. (1.91), Myristica fragrans Houtt. (1.53), Kokoona zeylanica Thwaites (1.37), Indigofera tinctoria L. (1.30), Citrus aurantifolia Panzer. (Christm.) Swingle (1.30), Centella asiatica (L.) Urb (1.27), Coscinium fenestratum (Goetgh.) Colebr. (1.27), Azadirachta indica A.Juss (1.22), Cymbopogon citratus (DC.) Stapf (1.13), Terminalia bellirica (Gaertn.) Roxb. (1.12), Sesamum indicum L. (1.12), Citrus limon (L.) Osbeck (1.10), Eclipta prostrata (L.) L (1.09), Theobroma cacao L. (1.09), Acorus calamus L. (1.05), Terminalia chebula Retz. (1.03), and Dillenia retusa Thunb. (1.00). High UVs indicate the frequent usage of these medicinal plant materials for cosmetic treatments by traditional practitioners and Ayurveda physicians with high use reports. Although the UV index was not calculated, ethnobotanical surveys conducted to find out medicinal plants used in anti-inflammatory remedies [4], skin diseases [7], and snake bites treatments [24] of Sri Lanka revealed about greater usage of Azadirachta indica A.Juss., in skin diseases treatments, Citrus aurantifolia (Christm.) Swingle in snake bites treatments, and Coscinium fenestratum (Goetgh.) Colebr. in anti-inflammatory remedies, respectively. According to the survey on medicinal materials used in traditional systems of medicine in Sri Lanka, Aloe vera (L.) Burm.f., Santalum album L., Coscinium fenestratum (Goetgh.) Colebr., and Acorus calamus L. have been listed as heavily used medicinal plants in Sri Lanka. Moreover, Sesamum indicum L., Azadirachta indica A.Juss, Indigofera tinctoria L., Terminalia bellirica (Gaertn.) Roxb., Terminalia chebula Retz., Asparagus racemosus Willd., and Centella asiatica (L.) Urb have been listed as the most demanded plant materials which show heavy usage in the Sri Lankan traditional systems of medicine [23].


## 3.2.3. Relative Importance (RI)

There were 12 medicinal plants which recorded the RI value greater than 1. The highest RI value was reported fromAsparagus racemosus Willd. and Hibiscus rosa-sinensis L. It was 1.67. This was followed by Kaempferia galanga L. (1.57), Aloe vera (L.) Burm.f. (1.52), Myristica fragrans Houtt. (1.43), Citrus aurantifolia Panzer. (Christm.) Swingle (1.24), Acorus calamus L. (1.10), Eclipta prostrata (L.) L (1.10), Indigofera tinctoria L. (1.10), Jasminum grandiflorum L. (1.10), Phyllanthus emblica L. (1.10), and Morinda citrifolia L. (1.10). The lowest RI value was 0.48, and it was recorded from 73 medicinal plants. This may possibly be due to the less used categories and a few used attributes of these plants.


## 3.2.4. Factor Informant Consensus (FIC)

The results of the FIC (Table4) showed that treatments for wrinkles and foot health improvements (1.00) under the skin care category, treatments for damaged hair and scalp cooling (1.00) under the hair care category, and treatments for malodour of the mouth (0.96) under oral care category had the higher degree of consensus. All these values were comparatively high, thus indicating good agreement among traditional practitioners and Ayurveda physicians on the knowledge and consequent use of particular plants or plant materials to treat different beauty issues.Table 4
Factor informant consensus (FIC) for different beauty issues.
CategoryTreatments forNumber of plantsCitationsFICSkin careImproving skin complexion25630.61Pimples23800.72Freckles13730.83Skin discoloration9640.87Healing8780.91Aging7720.92Acne4640.95Skin dryness4820.96Exfoliating4180.82Soften the skin4430.93Malodour of the body2290.96Cleansing2820.99Improving skin health290.88Whitening2300.97Wrinkles1161.00Improving foot health121.00Hair careHealthy hair20880.78Dandruff11770.87Pediculosis9830.90Scalp and hair cleansing8870.92Hair fallen8720.90Grey hair6520.90Improving hair color4870.97Damaged hair1861.00Scalp cooling1221.00Oral careImproving oral hygiene34880.62Malodour of the mouth3480.96Medicinal plants are used in cosmetic treatments for various reasons. The close analysis of the survey revealed the medicinal plant usage in 16 skin-related issues, 9 hair-related issues, and 2 major oral-related issues. The majority of medicinal plants used in skin care treatments were used to improve skin complexion. It was reported as 25 medicinal plants. Treatment for pimples was the second largest treatment segment under the skin care which reported 23 medicinal plants in usage. Other than these main two treatments, herbal preparations were used to treat for freckles (13 plants), skin discoloration (9 plants), healing (8 plants), aging (7 plants) and acne, skin dryness, exfoliating and soften the skin (4 plants per each), malodour of the body, cleansing, skin health, and whitening (2 plants per each).Camellia sinensis (L.) Kuntze is used to treat for skin wrinkles. Furthermore, Ipomoea pes-caprae (L.) R. Br. is utilized to improve foot health. The most of hair care treatments were done for improving the healthy hair followed by dandruff treatments. It was reported as usage of 20 and 11 medicinal plants, respectively. Moreover, treatments were recorded for pediculosis (9 plants), cleansing and hair fallen (8 plants per each), grey hair (6 plants), and improving hair color (4 plants). Moreover, Centella asiatica (L.) Urb is used to treat damaged hair whilst Jasminum grandiflorum L. plant extracted oil is used for scalp cooling. Majority of treatments under oral care were reported for improving oral hygiene. Most of treatments of oral care were prepared as mouth wash. 34 medicinal plants used to improve oral hygiene were reported. Furthermore, Cryptolepis dubia (Burm.f.) M.R. Almeida, Saraca asoca (Roxb.) Willd., and Hibiscus abelmoschus L. are used to treat particularly for malodour of the mouth. In addition, 12 medicinal plant species which used as fragrant agent were also recorded.


## 3.2.5. Correlation Analysis

Correlations among different quantitative measures are indicated in Figure5. RFC was moderately correlated with UV (Figure 5(a), R2 = 0.69, P=0.0001) while high correlation was reported between RI and UV (Figure 5(b), R2 = 0.898, P=0.0001) and RFC and RI (Figure 5(c), R2 = 0.931, P=0.0001), giving good linearity between variables. Therefore, this is proven that identified plant species significantly have the same importance irrespective to the methodology employed.Figure 5
Correlation among different quantitative measures. (a) Correlation between RFC and UV. (b) Correlation between RI and UV. (c) Correlation between RFC and RI.
(a)(b)(c)


## 4. Conclusion

To our knowledge, this was the first survey carried out to identify cosmetic potential medicinal plants throughout the island, Sri Lanka. High RFC, UV, and FIC values of medicinal plants from the current study indicate the better consensus among traditional practitioners and Ayurveda physicians about the usage of medicinal plants or plant materials for cosmetic treatments. Neither toxicity nor toxicity removal methods of the identified plants or plant materials were mentioned during the discussions with Ayurveda physicians and traditional practitioners. Therefore, further studies on toxicity and safety of identified plants or plant materials are paramount for herbal cosmetic product development in the future. Assembling of multidisciplinary cooperation of botanists, chemists, toxicologists, researchers, and biologists is suggested to analyze the interesting functional properties, safety, efficacy, and effectiveness of documented cosmetic potential medicinal plants. The present ethnopharmacological study on medicinal plants used for topical cosmetic treatments by traditional practitioners and Ayurveda physicians of Sri Lanka will contribute to preserve the traditional knowledge of medicinal plants. Also, this information could ultimately be utilized for the development of the herbal cosmetic industry in Sri Lanka.



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*Source: 5599654-2021-06-28.xml*