# Artemisia spp.: An Update on Its Chemical Composition, Pharmacological and Toxicological Profiles

Authors: Javad Sharifi-Rad; Jesús Herrera-Bravo; Prabhakar Semwal; Sakshi Painuli; Himani Badoni; Shahira M. Ezzat; Mai M. Farid; Rana M. Merghany; Nora M. Aborehab; Mohamed A. Salem; Surjit Sen; Krishnendu Acharya; Natallia Lapava; Miquel Martorell; Bekzat Tynybekov; Daniela Calina; William C. Cho
Journal: Oxidative Medicine and Cellular Longevity
 (2022)

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## Abstract

Artemisia plants are traditional and ethnopharmacologically used to treat several diseases and in addition in food, spices, and beverages. The genus is widely distributed in all continents except the Antarctica, and traditional medicine has been used as antimalarial, antioxidant, anticancer, antinociceptive, anti-inflammatory, and antiviral agents. This review is aimed at systematizing scientific data on the geographical distribution, chemical composition, and pharmacological and toxicological profiles of the Artemisia genus. Data from the literature on Artemisia plants were taken using electronic databases such as PubMed/MEDLINE, Scopus, and Web of Science. Selected papers for this updated study included data about phytochemicals, preclinical pharmacological experimental studies with molecular mechanisms included, clinical studies, and toxicological and safety data. In addition, ancient texts and books were consulted. The essential oils and phytochemicals of the Artemisia genus have reported important biological activities, among them the artemisinin, a sesquiterpene lactone, with antimalarial activity. Artemisia absinthium L. is one of the most famous Artemisia spp. due to its use in the production of the absinthe drink which is restricted in most countries because of neurotoxicity. The analyzed studies confirmed that Artemisia plants have many traditional and pharmacological applications. However, scientific data are limited to clinical and toxicological research. Therefore, further research is needed on these aspects to understand the full therapeutic potential and molecular pharmacological mechanisms of this medicinal species.


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## Body

## 1. Introduction

The search and development of medicines from plant raw materials have been one of the important areas of human science for centuries [1]. A new breath of this scientific direction was given by the discovery of unique antimalarial agent artemisinin by the Chinese scientist Youyou Tu, for which she received the Nobel Prize in Physiology or Medicine in 2015. The source of this medicine was the Artemisia annua L., which has long been known in Chinese folk medicine [2]. This plant is not the only one of the well-known representatives of the Artemisia L. genus. Artemisia genus (Asteraceae), named after the Greek goddess of the hunt and fertility Artemis, is considered one of the most widely distributed genera all over the world [3] and unites more than 400 species of plants of various life forms (grasses, shrubs, and less often trees) [4]. Many of them are weeds, some are invasive in certain regions of the planet, and at the same time, some species are listed in the Red Book. Wide distribution, a variety of component composition, and the resulting wide range of pharmacological effects made plants of the Artemisia genus popular remedies of traditional medicine and ensured their study and subsequent introduction into official medicine [5–7].The medicinal and aromatic applications ofArtemisia are well known for a long time as it produces volatile oil which has applications in medicine, cosmetics, and food production [8]. Some species of Artemisia are edible [9], and others especially those grown in Korea have been traditionally applied in treating inflammations and ulcers. The most famous species around the world are A. annua and A. absinthium L. which are known for their uses in traditional medicine [10, 11]. In South Africa, Artemisia afra Jacq. ex Willd. is commonly used for treating inflammation, coughs, colds, malaria, fever, influenza, and diabetes [12], while, in North America, Artemisia dracunculus L. is widely used for the treatment of wounds and possesses antioxidant and antidiabetic activities [13, 14]. Furthermore, Artemisia vulgaris L. is used in traditional medicine and has several activities such as anticancer, hepatoprotective, antiepileptic, antimalarial, and insecticidal properties [15–19]. Other various species such as Artemisia nilagirica (C.B.Clarke) Pamp., A. dracunculus, Artemisia herba-alba Asso, A. armeniaca Lam., and Artemisia scoparia Waldst. & Kitam. possess significant therapeutic properties [20]. A. scoparia also has a long history in medicine, and it has been in clinics as a diuretic, choleretic, and hepatoprotective [21]. A. scoparia was used to treat hepatitis, jaundice, sores, pruritus, asthma, gastritis, and expel parasites and to treat spiders’ bites. A combination of A. scoparia, Gardenia jasminoides J. Ellis, and rhubarb (Rheum rhabarbarum L.) was reported to be a classical prescription for curing jaundice [21]. Currently, several biopharmaceutical products containing Artemisia extracts are available nowadays in the local markets to treat various diseases [22]. The purpose of the review is to systematize updated scientific data on the chemical composition and new insights in the pharmacological mechanisms of action and discusses the toxicological profiles of the Artemisia genus.


## 2. Methodology

Two biomedical literature databases were searched for this review: PubMed/MEDLINE and Web of Science using the following MeSH search terms: “Artemisia/chemistry,” “Phytochemicals,” “Artemisinins/pharmacology,, “Artemisinins/therapeutic use,” “Medicine, Traditional,” “Phytotherapy/methods,” “Plant Extracts/pharmacology,” “Plant Extracts/therapeutic use,” “Plant Oils /pharmacology,” “Plant Oils/therapeutic use,” “Structure-Activity Relationship,” “Animals,” and “Humans.” Inclusion criteria are as follows: preclinical and clinical studies on the sources, acquisition approaches, experimental pharmacology, toxicology, and safety data regardingArtemisia spp. were included. Both in vivo and in vitro pharmacological studies which underlie the molecular mechanism of action were included. Exclusion criteria are as follows: studies with data not relevant for the aim of this updated review, or poor quality of studies, duplicate studies. The taxonomy of plant species has been validated using the World Flora Online [23].


## 3. Geographical Distribution ofArtemisia spp.

The genusArtemisia is widely cosmopolitan and distributed worldwide except the Antarctica [24–26]. The genus is heterogeneous and inhabits from the sea level to high altitudes of around 4000 masl (meters above sea level) [27]. The species of Artemisia grows abundant in the Northern Hemisphere, and a low degree of colonization has been reported in the Southern Hemisphere [27, 28]. The main centre of species diversity of Artemisia is located in Central Asia consisting the region of Uzbekistan, Tadzhikistan, Turkmenistan, Kazakhstan, Kyrgyzstan, parts of Russia, China, and Mongolia. Other relevant centres of diversity include the territory of Iran-Turanian and Mediterranean regions and in western North America [29–33]. Artemisia has been spread beyond its native origin and successfully distributed and colonized in most of the arctic-alpine, temperate, and subtropical zones of the Northern Hemisphere. The distribution of the genus from Northern Asia primarily follows the main three routes: (1) in the West, it migrates into Europe, Western Asia, Mediterranean Basin, and Africa; (2) Siberia and into western North America; and (3) further south into Asia [34, 35]. Only a few number of species, not exceeding 25 taxa, have been reported from the Southern Hemisphere although a small diversity centre occurs in South America and it is found in Oceania as allochthonous taxa [32] (Figure 1).Figure 1
Geographical distribution ofArtemisia species.


## 4. Phytochemical Composition

### 4.1. Essential Oils

The essential oils (EOs) present in botanicals have been used for centuries in the form of spices, medicines, and their pleasant odour [36]. It has been possible only due to the development of distillation techniques in the middle ages and is used in their ancient applications in food, drugs, or cosmetics [37]. While, in the last decades, the EO industry entered different sectors with new dimensions and targets due to its various therapeutic applications. The chemical composition of Artemisia genus EOs has been reported by several authors around the world. The composition of EOs varies depending on several factors including the plant part, growing season, age of the plant, location, extraction techniques, solvent, and timing [38]. The detailed investigations on the EO composition of the Artemisia genus from different geographical regions have been presented in Table 1.Table 1
Essential oil composition of theArtemisia genus from different geographical regions (2017–2021).
Plant speciesParts usedChemical compositionRegion/countryReferencesArtemisia absinthium L.LCamphor; E-caryophyllene; eucalyptol; germacrene D;α-cadinolBrazil[39]Artemisia anethoides Mattf.AP1,8-Cineole; terpinen-4-ol; 2-isopropyltoluene; pinocarveolChina[40]Artemisia annua L.APArtemisia ketone;α-caryophyllene; germacrene DChina[41]AP, F, L(E)-β-Farnesene; germacra-4(15),5,10(14)-trien-1-ol; Artemisia alcohol 3-methyl butanoate; yomogi alcohol; Artemisia alcohol 3-phenylpropionate; Artemisia alcohol 2-methyl butanoate; α-copaene; artemisia alcohol; 1,8-cineolRussia[42]Artemisia arborescens (Vaill.) L.F, Lβ-Thujone, camphor, terpinen-4-ol, germacrene D, chamazuleneItaly[43]Artemisia argyi H. Lév. & VaniotLα-Thujone; bornanone; terpinen-4-ol; cis-2-menthenol; borneol; cis-sabino; α-terpineol; β-caryophyllene; caryophyllene oxide; neointermedeolChina[44]Artemisia campestris L.—β-Pinene; cadin-4-en-7-ol; Z-β-ocimene; γ-terpinenePortugal[45]APβ-Pinene; α-pinene; myrcene; germacrene D; (Z)-β-ocimene; γ-curcumeneAlgeria[46]APβ-Pinene; spathulenol; α-pinene; limonene; o-cymeneMorocco[47]L, Sβ-Pinene; 2-undecanone; limonene; benzene; α-pinene; 1,4-cyclohexadiene; β-myrcene; 2-naphthalenemethnol; 2-decanoneTunisia[48]Artemisia dracunculus L.APp-Allylanisole; ocimene (e)-β; ocimene (z)-β; limoneneIran[49]LMethyl eugenol; elemicin; isoelemicin; (Z)-methyl isoeugenolPoland[50]Artemisia gmelinii Weber ex Stechm.—γ-Amorphene; isohumbertiol B; caryophyllene oxide; caryophylla-4 (12), 8(13)-diene-5α-ol; ylangenol; caryophyllene; cabrevia oxide BRussia[51]APCyclobutane ethanol; endo-borneol; germacrene D; eucalyptol; selin-6-en-4α-ol; bisabolone oxide A; caryophyllene; terpinen-4-olChina[52]APPhellandrene; ascaridole;α-terpinolene; isoascaridole; benzyl isovalerateIndia[53]Artemisia herba-alba AssoAPcis-Thujone; trans-thujone; vanillyl alcohol; nordavanone; cis, threo-davanafuranMorocco[54]AP3-Thujanone a; 3-thujanone b; camphorSweden[55]Lα-Thujone; germacrene D; 1,8-cineole; β-thujoneTunisia[56]Artemisia jordanica DaninL2,3-Dehydro-1,8-Cineole; camphene; endo-borneol; bornyl acetate; geranyl isovaleratePalestine[57]Artemisia judaica L.APMethyl pentanoate; (E)-salvene; santolina triene; allyl isovalerate;α-pinene; β-citronellene; camphene; benzaldehyde; myrcene; mesitylene; yomogi alcohol; 1,4-cineol; α-terpinene; artemisia ketone; 2,6-dimethyl phenol; chrysanthenone; camphor; artemisyl acetate; piperitone; (Z)-ethyl cinnamate; (E)-ethyl cinnamate; germacrene D; davanoneJordan[58]APButanoic acid;β-linalool; 2-cyclohexen-1-one, 3-methyl-6-(1-methylethyl); acenaphthene; davana etherSaudi Arabia[59]Artemisia magellanica Sch.Bip.APγ-Costol; (Z)-en-yn-dicycloether; α-selinene; selina-4,11-diene (eudesma-4,11-diene); (E)-β-farnesene; 2-methylbutyl 2-methylbutyrate; (Z)-β-ocimeneArgentina[60]Artemisia monosperma DelileAPSpathulenol; cloven;β-linalool; α-citral; geranyl acetate; isohomogenol; benzene, 1,2-dimethoxy-4-(1-propenyl); caryophyllene; aristolene; 2-propenoic acid, 3-phenyl-ethyl esterSaudi Arabia[59]Lβ-Pinene; limonene; cis-β-ocimene; α-terpinolene; cis-sabinene hydrate; bornyl acetateSaudi Arabia[61]Artemisia nilagirica (C.B.Clarke) Pamp.APβ-Thujone; germacrene-D; β-thujone; caryophyllene; caryophyllene oxide; borneolIndia[62]Artemisia pedemontana Balb.F, Lα-Pinene; camphene; p-cymene; 1,8-cineole; linalool; camphor; borneol; terpinen-4-ol; viridiflorol; 1-α-terpineolSpain[63]Artemisia persica Boiss.APLaciniata furanone E; artedouglasia oxide C; pinocarvone; trans-pinocarveol;α-pinene; 1,8-cineole; artedouglasia oxide B and DIran[64]Artemisia sieversiana Ehrh.APSantolina triene;α-thujone; eucalyptol; α-sabinene; trans-2-menthen-1-ol; α-selinene, caryophyllene epoxideChina[65]Artemisia tournefortiana Rchb.—cis-Spiroether; Z-β-farnesene; trans-nerolidol; camphorIndia[66]Artemisia scoparia Waldst. & Kitam.APSpathulenol; acenaphthene; davana ether; 2-propenoic acid, 3-phenyl-ethyl esterSaudi Arabia[59]Artemisia sieberi BesserAPAcenaphthene; 2-cyclohexen-1-one, 3-methyl-6-(1-methylethyl)Saudi Arabia[59]Artemisia vulgaris L.APCaryophyllene; humulene; germacrene D; borneol; caryophyllene oxideBrazil[67]APGermacrene D; 1,8-cineole;β-pinene; sabinene; cis-thujone; β-caryophyllene; caryophyllene oxide; α-humulene; davanoneLithuania[68]AP: areal part; L: leaves; F: flowers; —: not reported.


### 4.2. Other Bioactive Compounds

The phytochemical diversity assessment of theArtemisia genus exhibited the presence of different types of secondary metabolites reported by several authors around the world (Table 2).Table 2
Chemical composition ofArtemisia genus from different geographical regions.
Plant speciesChemical compositionRegion/countryReferencesArtemisia abrotanum L.Caffeic acid; chlorogenic acid; isochlorogenic acid; protocatechuic acid; rosmarinic acid; quercitrinSaudi Arabia[69]Artemisia absinthium L.Artemisinin;α-thujone; β-thujone; bornyl acetate; 4-terpineol; camphene; chamazulene; cadinene; myrcene; guaiazulene; linalool; γ-terpinene-[11]Artemisia annua L.Arteannuin B; artemisinin; artemisinic acid; scopoletinChina[70]Artemisia campestris L.Catechin; vanillic acid; caffeic acid; syringic acid; p-coumaric acid; gallic acidMorocco[71]Artemisia capillaris Thunb.Neochlorogenic acid; chlorogenic acid; cryptochlorogenic acid; caffeic acid; 1,3-dicaffeoylquinic acid; 3,4-dicaffeoylquinic acid; 3,5-dicaffeoylquinic acid; 4,5-dicaffeoylquinic acidChina[72]Artemisia dubia L. ex B.D.Jacks.Calotropoleanyl ester;α-amyrin; nonacosanoic acid; docosanoic acid; tetracosanoic acid; 1-(O-tricosanoyl) glycerol; 1-(O-pentacosanoyl) glycerol; β-sitosterolPakistan[73]Artemisia gmelinii Weber ex Stechm.Coumarins; phenolics; flavonoids; caffeoylquinic acids; diterpene glycosidesKorea[74]Artemisia herba-alba AssoCamphor; hanphillin; alkhanin; terpinen-4-ol;α-santonin; α-thujone; β-thujone; 2,5-bornanedioneMorocco[75]Artemisia indica Willd.5-Hydroxy-3,7,4′-trimethoxyflavone; ludartin; maackiain; lupeol; cis-matricaria ester; trans-matricaria ester; 6-methoxy-7,8-methylenedioxy coumarinChina[76]Artemisia lactiflora Wall. ex DC.β-Sitosterol; daucosterol; umbelliferone; isofraxidin; scopoletin; fraxidin; mandshurin; fraxin-8-O-β-D-glucopyranoside; euoniside; scopolin; 5,8-dihydroxy-7,4′-dimethoxy-flavone; syringing; chrysoeriol; tricin; luteolin; acacetin; apigenin; 5,7-dihydroxy-3,6,4′-trimethoxy-flavone; tectorigenin; eicosyl/docosyl-p-coumarate; isoferulic acid; ferulaldehyde; ethyl caffeate; caffeic acid; (−)-syringaresinol; (+)-diasyringaresinol; p-hydroxybenzoic acid; p-methylbenzaldehyde; cleomiscosin C; cleomiscosin A or B; biisofraxidinChina[77]Artemisia mongolica (Fisch. ex Besser) Fisch. ex Nakai1-(3-Hydroxyphenyl)-2-(5-hydroxy-3-methoxyphenyl)ethane; 1-(3-hydroxyphenyl)-2-(3,5-dihydroxyphenyl)ethaneChina[78]Artemisia myriantha Wall. ex BesserBlumenol A; (+)-dehydrovomifoliol; (+)-3-hydroxy-β-ionone; (3R, 6R, 7E)-3-hydroxy-4, 7-megastigmadien-9-one; (−)-10-oxo-isodauc-3-en-15-oic acid; isoerivanin; eudesmafraglaucolide; artanomalide A; 13-acetoxy-3β-hydroxy-germacra-1(10) E,4E,7(11)-trien-12,6α-olide; 13-acetoxy-3β-tigloyl-germacra-1(10) E, 4E, 7(11)-trien-12, 6α-olide (10),13-acetoxy-3β-(3-methylbutanoyl)-germacra-1(10)E, 4E, 7(11)-trien-12, 6α-olide (11),3,9-diacetoxy-13-hydroxy-1(10), 4, 7(11)-germacratrien-12,6α-olide; 8α-angeloyloxycostunolideChina[79]Artemisia pontica L.n-Hexadecanoic acid; 9,12,15-(Z,Z,Z)-octadecatrienoic acid; 2-(4a,8-dimethyl-7-oxo-1,2,3,4,4a,7-hexahydronaphthalen-2-yl)-propionic acid; 8-nitro-(1H)quinolin-4-ol-2-one; neophytadieneUkraine[80]Artemisia rupestris L.Citrusin A; alaschanioside A; coniferin; citrusin B; syringaresinol-β-D-glucoside; (6R,9S)-3-carbonyl-α-ionol glucopyranoside; byzantionoside BChina[81]Artemisia scoparia Waldst. & Kitam.Eugenol; capillene; spathulenol; capillin; scoparone; tricosane; heptacosane; nonacosane; stigmasterol; tritriacontaneSerbia[82]4-Pyridone glucoside; polyacetylene glucosidesChina[83]Quercetin-3-O-β-d-glucoside; 3,4-dihydroxy-5-methoxycinnamic acid; caffeic acid; 6,7 dimethoxycoumarinChina[84]Artemisia splendens Willd.Narcisin; quercetin; luteolin; kaempferol; genkwanin; astragalin; isorhamnetin-3-O-β-D-glucosideIran[85]Artemisia turanica Krasch.3,5-Dicaffeoylquinic acid; 4,5-dicaffeoylquinic acid; 3,5,3′,4′-tetrahydroxy; 7,5′-methoxyflavonesIran[86, 87]Artemisia vulgaris L.Artanoic acid; luteolin; 6-methoxyluteolin; eupatilin; o-coumaric acid; vanillic acid; protocatechuic acid; 4-hydroxyphenyl acetate; vulgarinVietnam[88]


## 4.1. Essential Oils

The essential oils (EOs) present in botanicals have been used for centuries in the form of spices, medicines, and their pleasant odour [36]. It has been possible only due to the development of distillation techniques in the middle ages and is used in their ancient applications in food, drugs, or cosmetics [37]. While, in the last decades, the EO industry entered different sectors with new dimensions and targets due to its various therapeutic applications. The chemical composition of Artemisia genus EOs has been reported by several authors around the world. The composition of EOs varies depending on several factors including the plant part, growing season, age of the plant, location, extraction techniques, solvent, and timing [38]. The detailed investigations on the EO composition of the Artemisia genus from different geographical regions have been presented in Table 1.Table 1
Essential oil composition of theArtemisia genus from different geographical regions (2017–2021).
Plant speciesParts usedChemical compositionRegion/countryReferencesArtemisia absinthium L.LCamphor; E-caryophyllene; eucalyptol; germacrene D;α-cadinolBrazil[39]Artemisia anethoides Mattf.AP1,8-Cineole; terpinen-4-ol; 2-isopropyltoluene; pinocarveolChina[40]Artemisia annua L.APArtemisia ketone;α-caryophyllene; germacrene DChina[41]AP, F, L(E)-β-Farnesene; germacra-4(15),5,10(14)-trien-1-ol; Artemisia alcohol 3-methyl butanoate; yomogi alcohol; Artemisia alcohol 3-phenylpropionate; Artemisia alcohol 2-methyl butanoate; α-copaene; artemisia alcohol; 1,8-cineolRussia[42]Artemisia arborescens (Vaill.) L.F, Lβ-Thujone, camphor, terpinen-4-ol, germacrene D, chamazuleneItaly[43]Artemisia argyi H. Lév. & VaniotLα-Thujone; bornanone; terpinen-4-ol; cis-2-menthenol; borneol; cis-sabino; α-terpineol; β-caryophyllene; caryophyllene oxide; neointermedeolChina[44]Artemisia campestris L.—β-Pinene; cadin-4-en-7-ol; Z-β-ocimene; γ-terpinenePortugal[45]APβ-Pinene; α-pinene; myrcene; germacrene D; (Z)-β-ocimene; γ-curcumeneAlgeria[46]APβ-Pinene; spathulenol; α-pinene; limonene; o-cymeneMorocco[47]L, Sβ-Pinene; 2-undecanone; limonene; benzene; α-pinene; 1,4-cyclohexadiene; β-myrcene; 2-naphthalenemethnol; 2-decanoneTunisia[48]Artemisia dracunculus L.APp-Allylanisole; ocimene (e)-β; ocimene (z)-β; limoneneIran[49]LMethyl eugenol; elemicin; isoelemicin; (Z)-methyl isoeugenolPoland[50]Artemisia gmelinii Weber ex Stechm.—γ-Amorphene; isohumbertiol B; caryophyllene oxide; caryophylla-4 (12), 8(13)-diene-5α-ol; ylangenol; caryophyllene; cabrevia oxide BRussia[51]APCyclobutane ethanol; endo-borneol; germacrene D; eucalyptol; selin-6-en-4α-ol; bisabolone oxide A; caryophyllene; terpinen-4-olChina[52]APPhellandrene; ascaridole;α-terpinolene; isoascaridole; benzyl isovalerateIndia[53]Artemisia herba-alba AssoAPcis-Thujone; trans-thujone; vanillyl alcohol; nordavanone; cis, threo-davanafuranMorocco[54]AP3-Thujanone a; 3-thujanone b; camphorSweden[55]Lα-Thujone; germacrene D; 1,8-cineole; β-thujoneTunisia[56]Artemisia jordanica DaninL2,3-Dehydro-1,8-Cineole; camphene; endo-borneol; bornyl acetate; geranyl isovaleratePalestine[57]Artemisia judaica L.APMethyl pentanoate; (E)-salvene; santolina triene; allyl isovalerate;α-pinene; β-citronellene; camphene; benzaldehyde; myrcene; mesitylene; yomogi alcohol; 1,4-cineol; α-terpinene; artemisia ketone; 2,6-dimethyl phenol; chrysanthenone; camphor; artemisyl acetate; piperitone; (Z)-ethyl cinnamate; (E)-ethyl cinnamate; germacrene D; davanoneJordan[58]APButanoic acid;β-linalool; 2-cyclohexen-1-one, 3-methyl-6-(1-methylethyl); acenaphthene; davana etherSaudi Arabia[59]Artemisia magellanica Sch.Bip.APγ-Costol; (Z)-en-yn-dicycloether; α-selinene; selina-4,11-diene (eudesma-4,11-diene); (E)-β-farnesene; 2-methylbutyl 2-methylbutyrate; (Z)-β-ocimeneArgentina[60]Artemisia monosperma DelileAPSpathulenol; cloven;β-linalool; α-citral; geranyl acetate; isohomogenol; benzene, 1,2-dimethoxy-4-(1-propenyl); caryophyllene; aristolene; 2-propenoic acid, 3-phenyl-ethyl esterSaudi Arabia[59]Lβ-Pinene; limonene; cis-β-ocimene; α-terpinolene; cis-sabinene hydrate; bornyl acetateSaudi Arabia[61]Artemisia nilagirica (C.B.Clarke) Pamp.APβ-Thujone; germacrene-D; β-thujone; caryophyllene; caryophyllene oxide; borneolIndia[62]Artemisia pedemontana Balb.F, Lα-Pinene; camphene; p-cymene; 1,8-cineole; linalool; camphor; borneol; terpinen-4-ol; viridiflorol; 1-α-terpineolSpain[63]Artemisia persica Boiss.APLaciniata furanone E; artedouglasia oxide C; pinocarvone; trans-pinocarveol;α-pinene; 1,8-cineole; artedouglasia oxide B and DIran[64]Artemisia sieversiana Ehrh.APSantolina triene;α-thujone; eucalyptol; α-sabinene; trans-2-menthen-1-ol; α-selinene, caryophyllene epoxideChina[65]Artemisia tournefortiana Rchb.—cis-Spiroether; Z-β-farnesene; trans-nerolidol; camphorIndia[66]Artemisia scoparia Waldst. & Kitam.APSpathulenol; acenaphthene; davana ether; 2-propenoic acid, 3-phenyl-ethyl esterSaudi Arabia[59]Artemisia sieberi BesserAPAcenaphthene; 2-cyclohexen-1-one, 3-methyl-6-(1-methylethyl)Saudi Arabia[59]Artemisia vulgaris L.APCaryophyllene; humulene; germacrene D; borneol; caryophyllene oxideBrazil[67]APGermacrene D; 1,8-cineole;β-pinene; sabinene; cis-thujone; β-caryophyllene; caryophyllene oxide; α-humulene; davanoneLithuania[68]AP: areal part; L: leaves; F: flowers; —: not reported.


## 4.2. Other Bioactive Compounds

The phytochemical diversity assessment of theArtemisia genus exhibited the presence of different types of secondary metabolites reported by several authors around the world (Table 2).Table 2
Chemical composition ofArtemisia genus from different geographical regions.
Plant speciesChemical compositionRegion/countryReferencesArtemisia abrotanum L.Caffeic acid; chlorogenic acid; isochlorogenic acid; protocatechuic acid; rosmarinic acid; quercitrinSaudi Arabia[69]Artemisia absinthium L.Artemisinin;α-thujone; β-thujone; bornyl acetate; 4-terpineol; camphene; chamazulene; cadinene; myrcene; guaiazulene; linalool; γ-terpinene-[11]Artemisia annua L.Arteannuin B; artemisinin; artemisinic acid; scopoletinChina[70]Artemisia campestris L.Catechin; vanillic acid; caffeic acid; syringic acid; p-coumaric acid; gallic acidMorocco[71]Artemisia capillaris Thunb.Neochlorogenic acid; chlorogenic acid; cryptochlorogenic acid; caffeic acid; 1,3-dicaffeoylquinic acid; 3,4-dicaffeoylquinic acid; 3,5-dicaffeoylquinic acid; 4,5-dicaffeoylquinic acidChina[72]Artemisia dubia L. ex B.D.Jacks.Calotropoleanyl ester;α-amyrin; nonacosanoic acid; docosanoic acid; tetracosanoic acid; 1-(O-tricosanoyl) glycerol; 1-(O-pentacosanoyl) glycerol; β-sitosterolPakistan[73]Artemisia gmelinii Weber ex Stechm.Coumarins; phenolics; flavonoids; caffeoylquinic acids; diterpene glycosidesKorea[74]Artemisia herba-alba AssoCamphor; hanphillin; alkhanin; terpinen-4-ol;α-santonin; α-thujone; β-thujone; 2,5-bornanedioneMorocco[75]Artemisia indica Willd.5-Hydroxy-3,7,4′-trimethoxyflavone; ludartin; maackiain; lupeol; cis-matricaria ester; trans-matricaria ester; 6-methoxy-7,8-methylenedioxy coumarinChina[76]Artemisia lactiflora Wall. ex DC.β-Sitosterol; daucosterol; umbelliferone; isofraxidin; scopoletin; fraxidin; mandshurin; fraxin-8-O-β-D-glucopyranoside; euoniside; scopolin; 5,8-dihydroxy-7,4′-dimethoxy-flavone; syringing; chrysoeriol; tricin; luteolin; acacetin; apigenin; 5,7-dihydroxy-3,6,4′-trimethoxy-flavone; tectorigenin; eicosyl/docosyl-p-coumarate; isoferulic acid; ferulaldehyde; ethyl caffeate; caffeic acid; (−)-syringaresinol; (+)-diasyringaresinol; p-hydroxybenzoic acid; p-methylbenzaldehyde; cleomiscosin C; cleomiscosin A or B; biisofraxidinChina[77]Artemisia mongolica (Fisch. ex Besser) Fisch. ex Nakai1-(3-Hydroxyphenyl)-2-(5-hydroxy-3-methoxyphenyl)ethane; 1-(3-hydroxyphenyl)-2-(3,5-dihydroxyphenyl)ethaneChina[78]Artemisia myriantha Wall. ex BesserBlumenol A; (+)-dehydrovomifoliol; (+)-3-hydroxy-β-ionone; (3R, 6R, 7E)-3-hydroxy-4, 7-megastigmadien-9-one; (−)-10-oxo-isodauc-3-en-15-oic acid; isoerivanin; eudesmafraglaucolide; artanomalide A; 13-acetoxy-3β-hydroxy-germacra-1(10) E,4E,7(11)-trien-12,6α-olide; 13-acetoxy-3β-tigloyl-germacra-1(10) E, 4E, 7(11)-trien-12, 6α-olide (10),13-acetoxy-3β-(3-methylbutanoyl)-germacra-1(10)E, 4E, 7(11)-trien-12, 6α-olide (11),3,9-diacetoxy-13-hydroxy-1(10), 4, 7(11)-germacratrien-12,6α-olide; 8α-angeloyloxycostunolideChina[79]Artemisia pontica L.n-Hexadecanoic acid; 9,12,15-(Z,Z,Z)-octadecatrienoic acid; 2-(4a,8-dimethyl-7-oxo-1,2,3,4,4a,7-hexahydronaphthalen-2-yl)-propionic acid; 8-nitro-(1H)quinolin-4-ol-2-one; neophytadieneUkraine[80]Artemisia rupestris L.Citrusin A; alaschanioside A; coniferin; citrusin B; syringaresinol-β-D-glucoside; (6R,9S)-3-carbonyl-α-ionol glucopyranoside; byzantionoside BChina[81]Artemisia scoparia Waldst. & Kitam.Eugenol; capillene; spathulenol; capillin; scoparone; tricosane; heptacosane; nonacosane; stigmasterol; tritriacontaneSerbia[82]4-Pyridone glucoside; polyacetylene glucosidesChina[83]Quercetin-3-O-β-d-glucoside; 3,4-dihydroxy-5-methoxycinnamic acid; caffeic acid; 6,7 dimethoxycoumarinChina[84]Artemisia splendens Willd.Narcisin; quercetin; luteolin; kaempferol; genkwanin; astragalin; isorhamnetin-3-O-β-D-glucosideIran[85]Artemisia turanica Krasch.3,5-Dicaffeoylquinic acid; 4,5-dicaffeoylquinic acid; 3,5,3′,4′-tetrahydroxy; 7,5′-methoxyflavonesIran[86, 87]Artemisia vulgaris L.Artanoic acid; luteolin; 6-methoxyluteolin; eupatilin; o-coumaric acid; vanillic acid; protocatechuic acid; 4-hydroxyphenyl acetate; vulgarinVietnam[88]


## 5. Pharmacological Effects ofArtemisia spp. Extracts and Its Bioactives: Underlying Molecular Mechanisms

Artemisia spp. have a broad range of pharmacological activities such as antiulcer, anticancer, hepatoprotective, antidiabetic, antioxidant, and antimicrobial. Some of the preclinical studies of these species’ activities are summarized in Table 3 and Figure 2.Table 3
Preclinical pharmacological studies of differentArtemisia species.
Extract/compoundDosesIn vitro/in vivoRoute of administration/assayModel/cellsActivityPotential effectReferenceA. nilagirica/ethanolic extracts500 mg/kgIn vivoOrallyRatsAntiulcerGastroprotective, ↑proteins of mucus content[123, 124]A. nilagirica/methanolic extract150–250 mg/kgIn vivoOrallySwiss albino miceGastroprotective compared to standard drug vincristine[125]A. absinthium, A. vulgaris/flowers/methanolic extract62.5, 125, 250, 500μg/mLIn vitroMTTMCF7 cellsAnticancer↑cytotoxicityIC50=221–500 μg/mL[126]A. nilagirica/ethyl acetate, hexane fractions100μg/mLIn vitroSRBDLD-1 cells↑cytotoxicityIC50=15.42–23.4 μg/mL[127]A. vulgaris/leaves/methanolic extract0.01–1.0 mg /mLIn vitroMTTHepatocellular carcinoma cells↑apoptosisIC50=0.1mg/mL[15]A. absinthium/methanolic extract20, 25 g/mLIn vitroMTTMCF-7 MDA-MB231↑cancer cells suppression[108, 128]A. armeniaca/CH2Cl2 fraction6.25–200μg/mLIn vitroMTSApoptosis-proficient HL60 apoptosis-resistant K562HL-60:IC50=75μg/mL, K562: IC50=130μg/mL[129]A. dracunculus/aerial parts, roots/ethanol, aqueous extracts250 mg/kgIn vivoOrallySTZ-induced diabetic ratsAntidiabetic↓TGL, ↓LDL, ↓HDL[14]A. dracunculus L. (PMI 5011)/ethanolic extractPMI 5011 (1%)In vivoDietKK-Ay mice↑sensitivity of insulin, ↑insulin receptor signaling[130, 131]A. sieberi (A. herba-alba)/aqueous extracts0.39 g/kgIn vivoOrallyAlloxan-induced diabetic rats↓blood glucose, ↑RBC, ↑WBC, ↑PCV, ↑ESR, ↑neutrophils, ↓heart rate[132]A. persica/aqueous, methanolic extracts300, 400, 500 mg/kgIn vivoOrallySprague-Dawley ratsAntihypertensive↓systolic blood pressure in normotensive/hypertensive rats[133]A. absinthium/aqueous extract50, 100, 200 mg/kgIn vivoOrallyKunming mice, NIH miceHepatoprotective↓inflammatory cells, ↓liver lipid peroxidation, ↑SOD, ↑GPx[134]A. vulgaris/aerial parts/crude extract150, 300, 600 mg/kgIn vivoi.p.Balb-C mice↑liver structure, ↓parenchyma congestion, ↓cellular swelling, ↓apoptotic cells[16]A. nilagirica/leaf extracts32–512μg/mLIn vitroAgar disk diffusion method15 bacterial strainsAntibacterialMethanol, hexane extracts, ↑inhibition against phytopathogens[135]A. herba- alba, A. judaica, A. monosperma/EO10.0, 5.0, 2.5, 1.0, 0.5μL/discIn vitroAgar disc diffusion method.Staphylococcus aureus ATCC29213, Escherichia coli ATCC 25922IC50=0.5–2.5μLA. judaica, A. monosperma plants had the highest MIC[136]A. judaica/ethanol extract250, 500, 1000, 2000, 4000μg/mLIn vitro(mic90) growth inhibitionProtozoan parasite (blastocystis)AntiprotozoalIC5=4000μg/mL, ↓growth, ↑destruction of blastocystis[137]A. nilagirica/EO0.33μL/mLIn vitroInverted petri plate techniqueA. flavus, A. niger, A. ochraceusAntifungalIC50=1.6μL/mL, ↓fungal growth, ↓mycotoxin secretion, ↓aflatoxigenic, ↓ochratoxigenic strains[138]A. annua/leaves/EO ethanolic extractEO=470mg/kg ethanol extract=450mg/kgIn vivoi.p.Wistar ratsAntidepressant↑immobility time in the FST, ↓other activities in the OFT depressors of SNC[139]A. absinthium/aerial parts/methanolic extract125, 250, 500, 1000 mg/kgIn vivoi.p.Swiss albino mice↓immobility period in the fst and tst.dose-dependent antidepressant activity[140, 141]A. vulgaris/leaves/methanolic extract50, 100, 300 mg/kgIn vivoi.p.Swiss albino miceAntiepilepticAnticonvulsant activities were noticed using EPM and MBT[18]A. capillaris/herba/ethanolic extract50, 100, 200, 400 mg/kgIn vivoOrallyMiceAnticonvulsivant effect through the GABA-ergic neuron[142]A. nilagirica/leaves/ethanolic, aqueous extracts100, 200 mg/kgIn vivoi.p.Swiss albino miceAnti-AlzheimerConfirmation of the anti-Alzheimer’s activity of ethanol extract after object recognition and y-maze tests[143]A. nilagirica/leaves/ethanolic, aqueous extracts100, 200 mg/kgIn vivoi.p.Swiss albino miceAnti-Parkinson↓catalepsy score in animals treated with ethanolic extract, ↑locomotor activity, ↑rotarod readings[143]A. annua/aqueous, ethanolic extracts2 g/LIn vitroABTS, ORAC, FRAP—Antioxidant↑protection against the oxidative deterioration of oil-in-water emulsion[144]A. dracunculus L./leaves/methanolic extract20μLIn vitroDPPH—↑antioxidant activity by phenolics[13]A. nilagirica/leaves/ethanolic, aqueous extracts50–250μg/mLIn vitroDPPH—Antioxidant activity of ethanolicextract>aqueous extract[143]A. scoparia, A. spicigera/methanolic extracts0.25; 0.125; 0.0625; 0.0312; 0.0156; 0.0078; 0.0039; 0.0019; 0.0009; 0.00048 mg/mLIn vitroDPPH—↑free radical scavenging activityRC50=0.03157, 0.0456 mg/mL[145]A. nilagirica/EO10; 8.6; 6.5; 6.5; 3.3; 2.5; 2μg/mLIn vitroMethod recommended by WHOAedes albopictus mosquitoInsecticidalLC50=5μg/mL[146]A. nilagirica/EO, chloroform, petroleum ether methanolic extracts—In vitroMethod recommended by WHOAedes aegypti, Anopheles stephensi, Culex quinquefasciatus mosquito larvaeThe EO ofA. nilagirica was the most effective larvicide against A. stephensi larvae[147]A. aucheri/methanolic extract25, 50, 100 mg/mLIn vitroScolicidal testsEchinococcus granulosus↓effect on the protoscolices of hydatid cysts[148]A. vulgaris/ethanolic extract1, 5, 10, 50, 100, 500, 1000 ppmIn vitroMethod recommended by WHOAedes aegyptiLC50=65.8ppm in 1 h and 18.6 ppm in 24 h, ↓ A. aegypti in various stages of its lifecycle[19]A. scoparia, A. spicigera/n-hexane, DCM, MeOH extracts20, 40, 80 mg/mLIn vitroToxicity bioassayTribolium castaneum (red flour beetle)Insecticidal properties, ↑activity of DCM extract[145]A. scoparia/butanol fraction20 mg/siteIn vivoTopicallyBALB/C miceAntiatopic dermatitis↓clinical symptoms in a DNFB mouse model that induced lesions, ↓inflammatory cytokines[149]A. scoparia/aerial parts/methanolic extract150, 300 mg/kgIn vivo—Sprague-Dawley ratsNephroprotective↓DNA damages,dose=300mg/kg, ↓oxidative stress[150]A. capillaris Thunb/extract10 mg/mouse/dayIn vivoTopicallyDermatophagoides farinae-sensitized NC/NGA miceAnti-inflammatory, anti-atopic dermatitis↓dermatitis scores, ↓bleeding, ↓hyperkeratosis, ↓hypertrophy in the dorsal skin and ear of the epidermis, ↓histamine[151]A. pallens/aerial parts/methanolic extract200, 400 mg/kgIn vivoOrallyWistar ratsAnti-inflammatory, antioxidative↓level of hepatic enzymes, ↑renal antioxidant enzymes[152]A. vulgaris/leaf/ethanolic extract250, 500, 750, 1000 mg/kgIn vivoOrallyICR mice infected withP. bergheiAntimalarial↓P. berghei, nontoxic[17]A. scoparia, A. spicigera/dichloromethane extracts0–2 mg/mL 10% DMSOIn vitroHeme biocrystallization and inhibition assayIC50=0.778mg/mL, IC50=0.998mg/mL[145]A. annua/aqueous, hydro alcoholic extracts—In vitroParasite lactate dehydrogenase (pLDH)Plasmodium falciparumIC50=3.27nM, IC50=4.95nM[153]A. annua/aqueous hydro alcoholic extractsAqueous extract 1000 mg/kg/day, hydro alcoholic extract 500 mg/kg/dayIn vivo-Plasmodium berghei NK173-infected m ice↑activity on malaria of artemisinin, both extracts ofA. annua are effective on malaria[153]↑: increase; ↓: decrease; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide; DCM: dichloromethane; DNFB: 2,4-dinitrofluorobenzene; EPM: elevated plus maze; ESR: erythrocyte sedimentation rate; EO: essential oil; FST: forced swimming test; HDL: high-density lipoprotein; LDL: low-density lipoprotein; MBT: marble-burying test; MIC: minimum inhibitory concentration; OFT: open-field test; PCV: packed cell volume; RBC: red blood cell; SRB: sulforhodamine B; TC: total cholesterol; TGL: triglycerides; WBC: white blood cell; WHO: World Health Organization: i.p.: intraperitoneally; FRAP: ferric-reducing ability of plasma; ABTS: 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid; ORAC: oxygen radical absorbance capacity.Figure 2
Illustrative scheme with the most representative pharmacological properties of bioactives ofArtemisia spp. and their potential mechanisms of action. ↑: increase; ↓: decrease; SOD: superoxide dismutase; GSH: glutathione; MEK/ERK: mitogen-activated extracellular signal-regulated kinase/extracellular signal-regulated protein kinase; Bcl-2: B-cell lymphoma 2; iNOS: inducible nitric oxide synthase; PGE2: prostaglandin E2; COX-2: cyclooxygenase-2; NF-κB: nuclear factor kappa B; TNF-α: tumor necrosis factor α; AMPK: adenosine monophosphate-activated protein kinase; AST: aspartate aminotransferase; GLUT4: glucose transporter type 4; AchE: acetylcholinesterase; MAO: monoamine oxidase; ROS: reactive oxygen species; PKC: protein kinase C; JNK: Jun N-terminal kinase; ALT: alanine transaminase; TTG: tissue transglutaminase antibody.One of the available famous drugs derived fromArtemisia species is artemisinin which exists in the leaves and flowers of A. annua. Other species which are known free of artemisinin were found to be active against malaria as A. vulgaris, A. absinthium, A. dracunculus, and A. scoparia; this activity was attributed to EOs and other sesquiterpenes [89]. Moreover, other studies also mentioned that artemisinin was not the only antimalarial substance in A. annua extracts [90–92].


### 5.1. Antioxidant

Antioxidants are a group of compounds that can help support the integrity of cells in the face of free radicals, unstable molecules that our body inevitably produces [93–95]. Natural antioxidants are thus essential for the proper functioning of the body [96–98]. Several studies have been reported the antioxidant activity of A. absinthium. Phenolic compounds (gallic acid, coumaric acid, vanillic acid, syringic acid, and chlorogenic salicylic acid) and flavonoids (quercetin and rutin) present in A. absinthium showed the potential of this plant against diseases related to oxidative stress [99–101]. These compounds reduce lipid peroxidation (thiobarbituric acid-reactive substances (TBARS) and recover endogenous antioxidant (e.g., superoxide dismutase (SOD) and glutathione (GSH)).


### 5.2. Anti-inflammatory

Inflammation is the body’s natural response to protecting itself and recovering from an injury [102, 103]. It has the function of protecting the body from harmful substances and regenerating the damaged tissue [1, 104, 105]. A. absinthium extracts exhibit anti-inflammatory properties which may be linked to its secondary metabolites including flavonoids and sesquiterpene-type compounds and their role in inflammatory regulator inhibition such as bradykinins, histamine, prostaglandins, and serotonin [106] and through suppression of proinflammatory mediator expression such as inducible nitric oxide synthase (iNOS), prostaglandin E-2 (PGE2), cyclooxygenase-2 (COX-2), factor nuclear factor-kappa-B (NF-κB), and tumor necrosis factor-α (TNF-α) [11].


### 5.3. Anticancer

Cancer is a disease in which the body’s cells grow uncontrollably, forming a tumor that can spread to different parts of the body [107–111]. The mechanism of the anticancer effect A. absinthium extract was due to the activation of the mitogen-activated protein kinase/extracellular signal-regulated kinase MEK/ERK signaling pathway, which in turn stimulates the mitochondrial pathway of caspase activation and regulates Bad and Bcl-2 family proteins, resulting in the apoptotic death of MCF-7 and MDA-MB231 human cancer cells [108].


### 5.4. Neuroprotective

Neurocerebral disorders, especially neurodegenerative ones, refer to several progressive brain syndromes that affect memory, thinking ability, behavior, and emotions [112–115]. A. absinthium has been shown to have neuroprotective effects on cerebral damage caused by reperfusion through its nicotinic and muscarinic action. The protective mechanism of ethanolic extract of A. absinthium may be due to its anticholinesterase activity as well as the ability to change the behavior of rats by restoring acetylcholinesterase (AChE) and monoamine oxidase (MAO) enzymes to near-normal activity [11]. The sesquiterpenoid dimer—caruifolin D—found in A. absinthium may be used for the treatment of neurodegenerative diseases such as Alzheimer’s or Parkinson’s due to its inhibitory action on the production of neuroinflammatory mediators in BV2 microglial cells and the reactive oxygen species (ROS) production; leading to inhibitory effects on the activations of protein kinase C (PKC) and c-Jun N-terminal kinase (JNK) [116].


### 5.5. Hepatoprotective

A. absinthium hydroalcoholic extract improves hepatic function and lowers oxidative stress indicators and consistently stimulates and preserves the structural morphology of the hepatocellular membrane, resulting in lower serum aspartate (ASAT) and alanine aminotransferase (ALAT) activity. The proposed hepatoprotective mechanisms include liver microsomal drug-metabolizing enzyme suppression, free radical scavenging activity, and calcium channel blockage [117].


### 5.6. Antidiabetic

Diabetes is a metabolic disease that causes excess blood glucose (hyperglycemia) [118]. This disease is incurable and once diagnosed requires lifelong treatment [119]. A. absinthium extracts showed an insulin-sensitizing action due to their role in adenosine monophosphate-activated protein kinase (AMPK) stimulation and glucose transporter type 4 (GLUT4) translocation to the cell surface of the muscle [120]. In diabetic rats treated with A. absinthium, the metabolic pathway shifted towards carbohydrate as a source of energy, preserving proteins and lipids while increasing their production, leading to preventing body weight loss [121].


### 5.7. Antimalarial

A sesquiterpene lactone, artemisinin, which is the main active ingredient inA. annua is used for the treatment of Plasmodium parasites; these parasites are characterized by their substantial hemoglobin uptake and digestion. This produces large quantities of free redox-active heme and free ferrous iron (Fe2+), which are assumed to be responsible for artemisinin’s parasite specificity. Infected erythrocytes convert excess heme to hematin, which is toxic to the parasite due to oxidative damage and direct cell membrane rupture; but malarial parasites have evolved a detoxification mechanism that uses a biocrystallization process to convert hematin to the less toxic and inert crystallized hemozoin. Activated artemisinin has been shown to inhibit the development of hemozoin by alkylating heme. As a result, artemisinin’s activator and target are both free heme from hemoglobin breakdown [122].


## 5.1. Antioxidant

Antioxidants are a group of compounds that can help support the integrity of cells in the face of free radicals, unstable molecules that our body inevitably produces [93–95]. Natural antioxidants are thus essential for the proper functioning of the body [96–98]. Several studies have been reported the antioxidant activity of A. absinthium. Phenolic compounds (gallic acid, coumaric acid, vanillic acid, syringic acid, and chlorogenic salicylic acid) and flavonoids (quercetin and rutin) present in A. absinthium showed the potential of this plant against diseases related to oxidative stress [99–101]. These compounds reduce lipid peroxidation (thiobarbituric acid-reactive substances (TBARS) and recover endogenous antioxidant (e.g., superoxide dismutase (SOD) and glutathione (GSH)).


## 5.2. Anti-inflammatory

Inflammation is the body’s natural response to protecting itself and recovering from an injury [102, 103]. It has the function of protecting the body from harmful substances and regenerating the damaged tissue [1, 104, 105]. A. absinthium extracts exhibit anti-inflammatory properties which may be linked to its secondary metabolites including flavonoids and sesquiterpene-type compounds and their role in inflammatory regulator inhibition such as bradykinins, histamine, prostaglandins, and serotonin [106] and through suppression of proinflammatory mediator expression such as inducible nitric oxide synthase (iNOS), prostaglandin E-2 (PGE2), cyclooxygenase-2 (COX-2), factor nuclear factor-kappa-B (NF-κB), and tumor necrosis factor-α (TNF-α) [11].


## 5.3. Anticancer

Cancer is a disease in which the body’s cells grow uncontrollably, forming a tumor that can spread to different parts of the body [107–111]. The mechanism of the anticancer effect A. absinthium extract was due to the activation of the mitogen-activated protein kinase/extracellular signal-regulated kinase MEK/ERK signaling pathway, which in turn stimulates the mitochondrial pathway of caspase activation and regulates Bad and Bcl-2 family proteins, resulting in the apoptotic death of MCF-7 and MDA-MB231 human cancer cells [108].


## 5.4. Neuroprotective

Neurocerebral disorders, especially neurodegenerative ones, refer to several progressive brain syndromes that affect memory, thinking ability, behavior, and emotions [112–115]. A. absinthium has been shown to have neuroprotective effects on cerebral damage caused by reperfusion through its nicotinic and muscarinic action. The protective mechanism of ethanolic extract of A. absinthium may be due to its anticholinesterase activity as well as the ability to change the behavior of rats by restoring acetylcholinesterase (AChE) and monoamine oxidase (MAO) enzymes to near-normal activity [11]. The sesquiterpenoid dimer—caruifolin D—found in A. absinthium may be used for the treatment of neurodegenerative diseases such as Alzheimer’s or Parkinson’s due to its inhibitory action on the production of neuroinflammatory mediators in BV2 microglial cells and the reactive oxygen species (ROS) production; leading to inhibitory effects on the activations of protein kinase C (PKC) and c-Jun N-terminal kinase (JNK) [116].


## 5.5. Hepatoprotective

A. absinthium hydroalcoholic extract improves hepatic function and lowers oxidative stress indicators and consistently stimulates and preserves the structural morphology of the hepatocellular membrane, resulting in lower serum aspartate (ASAT) and alanine aminotransferase (ALAT) activity. The proposed hepatoprotective mechanisms include liver microsomal drug-metabolizing enzyme suppression, free radical scavenging activity, and calcium channel blockage [117].


## 5.6. Antidiabetic

Diabetes is a metabolic disease that causes excess blood glucose (hyperglycemia) [118]. This disease is incurable and once diagnosed requires lifelong treatment [119]. A. absinthium extracts showed an insulin-sensitizing action due to their role in adenosine monophosphate-activated protein kinase (AMPK) stimulation and glucose transporter type 4 (GLUT4) translocation to the cell surface of the muscle [120]. In diabetic rats treated with A. absinthium, the metabolic pathway shifted towards carbohydrate as a source of energy, preserving proteins and lipids while increasing their production, leading to preventing body weight loss [121].


## 5.7. Antimalarial

A sesquiterpene lactone, artemisinin, which is the main active ingredient inA. annua is used for the treatment of Plasmodium parasites; these parasites are characterized by their substantial hemoglobin uptake and digestion. This produces large quantities of free redox-active heme and free ferrous iron (Fe2+), which are assumed to be responsible for artemisinin’s parasite specificity. Infected erythrocytes convert excess heme to hematin, which is toxic to the parasite due to oxidative damage and direct cell membrane rupture; but malarial parasites have evolved a detoxification mechanism that uses a biocrystallization process to convert hematin to the less toxic and inert crystallized hemozoin. Activated artemisinin has been shown to inhibit the development of hemozoin by alkylating heme. As a result, artemisinin’s activator and target are both free heme from hemoglobin breakdown [122].


## 6. Clinical Studies

Long traditional usage and functional preclinical studies of differentArtemisia species for the treatment of several diseases encouraged their clinical evaluation to support the evidence of their potential as antimalarial, antioxidant, anticancer, antinociceptive, anti-inflammatory, and antiviral agents [4].


### 6.1. Anti-inflammatory Activity

In a randomized double-blind clinical trial, oral treatments of 42 patients by Arthrem (supercritical CO2-extracted A. annua) at doses 150 mg and 300 mg or placebo twice daily for 12 weeks were tested for their efficacy on stiffness, pain, and functional limitations in osteoarthritis of the hip and knees. Results showed a significant decrease in visual analogue scale (VAS) score and improvement in WOMAC (Western Ontario and McMaster Universities Osteoarthritis) total score only at the low dose of 150 mg [154]. Afterwards, an open-label 6 month extension trial was proceeded to examine the safety of Arthrem in the long run (6 months). Results showed that Arthrem could be a safe and effective agent for osteoarthritis management [155]. In a similar study, 90 patients diagnosed with osteoarthritis applied 3% A. absinthium ointment, 3% A. absinthium liniment, or piroxicam gel (PG) on their knees for 4 weeks. Results showed that A. absinthium ointment revealed significant improvement in all parameters except for WTSS (total stiffness score), where A. absinthium liniment only reduced VAS and WTPS (total pain score) in week 4 with recurrence in week 6 when compared to PG that improved all the parameters with no recurrence [156]. Furthermore, in a randomized controlled clinical trial, 10 patients with Crohn’s disease administrated dried A. absinthium powder (750 mg three times daily) along with their basic therapy for 6 weeks, where another ten patients served as the control group. Results showed a significant reduction in serum TNF-α level and Crohn’s disease activity index (CDAI) scores as well as remission of symptoms in eight patients [157].In a further clinical study, a nasal spray containing an extract ofA. abrotanum mainly composed of EO (4 mg/mL) and flavonols (2.5 μg/mL) was established for treating 12 patients with allergic rhinitis. The EO fraction is composed of 1,8-cineole, linalool, and davanone, while the flavonol fraction contained centauredin, casticin, and quercetin dimethyl-ethers, which are well known for their anti-inflammatory effect. Most of the patients exhibited a significant reduction in nasal congestion, sneezing, and rhinorrhea as well as relief of eye symptoms when compared to the effect of anti-histamines [158].In the sight of cosmetics, twenty-five sensitive skin patients were selected for investigating the efficacy ofA. annua extract on skin reliving. Results showed that consuming cosmetics having A. annua extract for 4 weeks could improve the hydration degree of the cheek cuticle by 63.90%, reduce the transepidermal water loss by 21.51%, reduce the sensitized area by 77.47%, repair skin damage, and reduce redness [159].


### 6.2. Anticancer Activity

Interestingly, artemisinin and its derivatives were reported as potent antitumor compounds with high selectivity on cancer cells without any side effects on normal cells [160]. Their mechanism of action is based on the cleavage of their endoperoxide bridge by Fe2+ in cancerous cells and the production of ROS involved in apoptosis, DNA damage, autophagy, and cell cycle arrest G0/G1 of the cancerous cells. Additionally, they can suppress angiogenesis by inhibiting the secretion of vascular endothelial growth factor (VEGF), vascular endothelial growth factor receptor 2 (VEGFR2), and kinase insert domain receptor (KDR)/flk-1 in tumors, as well as affecting different signaling pathways and transcription factors related to tumor growth [161, 162]. Besides the antitumor effect of artemisinin and its derivatives against human cancer cell lines in vitro [160, 163, 164], different clinical studies were established to ensure their potency. For instance, in a pilot clinical trial at phase II, the anti-tumor effect of dihydroartemisinin (200 mg/day) was tested against advanced cervical carcinoma for 3 weeks in ten women. Results showed a significant reduction in vaginal discharge and pain, with no sign of severe toxicity, as well as improvement in overall signs and symptoms. These patients also exhibited a lower expression of epidermal growth factor receptor (EGFR) and Ki-67 oncogenes [165].Further, in a randomized, controlled clinical trial, artesunate (120 mg, once daily, IV) was combined with vinorelbine (25 mg/m2, once daily, IV) and cisplatin (25 mg/m2, once daily, IV) to treat patients with the advanced lung cancer stage. Results showed significant improvement in the survival rate and hindering of the progression time of the cancerous cell, devoid of extra side effects [166]. In a case report conducted by Singh and Verma [167], artesunate (50 mg) proved to reduce the tumor size of the larynx of a patient with stage II cancer by 70% following 2 weeks of treatment [167]. As well in another case report conducted by Berger et al. [168], the combination of artesunate (50 mg twice/day) with standard chemotherapy showed a significant reduction in death risk and stabilization of the disease case in 2 patients with metastatic uveal melanoma stage IV, when compared to the chemotherapy alone [168]. Furthermore, artemether (40 mg once/day) proved to exhibit a significant improvement in the computed tomography scan of a 75-year-old patient with pituitary macroadenoma. Results showed a decrease in the tumor’s density as well as an improvement in the overall clinical signs and symptoms [169].


### 6.3. Antidiabetic Activity

In a randomized double-blind clinical study, 24 patients with impaired glucose tolerance (IGT) administratedA. dracunculus (1000 mg) or placebo before breakfast and dinner for 90 days. Results showed a significant decrease in systolic blood pressure, glycated hemoglobin, and total insulin secretion as well as a significant increase in the high-density lipoprotein cholesterol level [170].In a dose-response clinical study, ethanolic extract ofArtemisia princeps Pamp. containing eupatilin and jaseocidin was investigated for its antidiabetic effect in 81 patients with hyperglycemia. Patients were randomized into four groups: negative control (lactose 2000 mg/day), positive control (pinitol 1140 mg/day), low-dose extract (2000 mg/day), and high-dose extract (4000 mg/day). Both doses significantly reduced glycated hemoglobin level, where the free fatty acid level in plasma was only lowered in patients administrated the high dose of the extract [171]. In a similar clinical trial, A. absinthium capsules (1 g/twice daily for 30 days) or placebo were administrated to 16 patients with type II diabetes. Results showed that A. absinthium reduced the glucose level by 32%, triglycerides by 10%, total cholesterol by 5%, and LDL level by 6% [172].


### 6.4. Antimalarial Activity

Malaria is considered the most common tropical disease that is provoked by certain parasites of the genusPlasmodium such as P. malariae, P. viva, P. falciparum, and P. ovale [173]. Artemisia species are famous for their content of sesquiterpene lactones that are responsible for the high therapeutic potential of the genus [173, 174]. For instance, artemisinin and its derivatives are the most common sesquiterpene lactones among the genus. Dihydroartemisinin (the active form inside the biological systems) is produced by reducing the lactone of artemisinin. While, alkylation of the hemiacetal group yields arteether and artemether, where artesunate is produced by acylation of the hemiacetal group with succinic acid [175]. Inside in vivo systems, all these derivatives are converted to dihydroartemisinin, where it possesses the highest activity, oral bioavailability, and tolerability with minimal side effects. These compounds are well known for their powerful activity against different species of Plasmodium as they contain the 1,2,4-trioxane moiety that may be responsible for the mechanism of action of the drugs [122]. The antimalarial activity of these compounds outcomes from the presence of Fe2+ after the Plasmodium hemolysis. This Fe2+ is utilized as a catalyst to open the peroxide bridge of the compound, leading to the formation of free radicals, alkylating Plasmodium proteins, and finally causing parasite death. Artemisinin also can perform its antimalarial activity by inhibiting PfATP6, an enzyme for the delivery of Ca2+ into vesicles of the parasite, which is critical for its development [122]. In a cluster-randomized clinical trial performed in different African countries, it was determined that rectal artesunate could take from 4 to 6 h to reduce parasite load, progression of the disease, and risk of death, so it can be a good choice for patients who cannot be treated orally [176]. Moreover, the WHO reviewed different clinical trials performed by the African Quinine Artesunate Malaria Trial multicentre on 5400 children under the age of 15 years with multidrug-resistant severe malaria and suggested IV artesunate (2.4 mg/kg once daily) as a choice to treat malaria [177].A meta-analysis study using single-patient data from different randomized, controlled trials was conducted to compare artemether and quinine in treating severeP. falciparum infection. Results showed that the death rate was significantly reduced with patients treated with artemether (14%) when compared to patients treated with quinine (17%). On the other hand, there was no difference between the 2 treatments in coma recovery, fever clearance, or the progress of neurological toxicity. However, the overall adverse outcomes of either death or neurological toxicity were significantly fewer in the artemether-treated group [178].In a double-blind, randomized, placebo-controlled clinical trial, artesunate was combined with sulfadoxine-pyrimethamine to test their efficacy in reducing the timing of malaria exposure during the infancy stage. Results showed that innate cells produced a balanced level of proinflammatory and regulatory cytokines around 2 years of age, which was accompanied by a lower risk of clinical malaria [179]. Further, it was reported that patients with uncomplicated malaria who administrated this combination therapy exhibited an 84.1% cure rate [180].Despite the potency of artesunate as an antimalarial drug, there are also two main antimalarial regimen options: dihydroartemisinin–piperaquine (DHA–PPQ) and artemether-lumefantrine (AL), which are considered as the first option for treating uncomplicatedP. falciparum malaria globally [181]. For instance, DHA–PPQ was reported to have high benefits in children with uncomplicated malaria in endemic countries [182]. On the other hand, the AL therapy (marketed as “Coartem”) could exert its antimalarial effect through opposing the erythrocytic stages of the parasite and so reducing the number of parasites. In addition, lumefantrine had a much-extended half-life time when combined with artemether and was supposed to clear residuals of the parasites [183].As malaria causes severe maternal and fetal problems, the Centers for Disease Control and Prevention (CDC) suggested AL therapy for treating pregnant women with uncomplicated malaria in the United States throughout the second and third trimesters of pregnancy, at the same doses assigned for nonpregnant women [184]. In a case report implemented by Daddy et al. [185], dried leaves of A. annua were investigated for their efficacy as an antimalarial agent in patients (with P. falciparum infection) not responding to artemisinin combination therapy (ACT) or artesunate (IV). After oral administration of dried leaves of A. annua at a dose (0.5 g/twice daily/5 days), these patients exhibited a subside in clinical symptoms and the parasites were undetected microscopically [185]. In a large-scale double-blind, randomized clinical trial, the antimalarial effect of A. annua and A. afra infusions (1 L/day of dry leaf/twig infusion for 7 days) was compared to artesunate-amodiaquine (ASAQ) by 957 patients with malaria (with P. falciparum infection). Results showed that patients treated with both Artemisia infusions exhibited trophozoites clearance after 24 h when compared to ASAQ which took up to 14 days. Moreover, fever clearance took up to 48 h for ASAQ, but only 24 h for both Artemisia infusions. From days 14–28, gametocytes were undetectable for patients treated with Artemisia infusions, whereas on day 28, ASAQ-treated patients stayed carriers for gametocytes. These results proved that Artemisia infusions could break the life cycle of malaria by eliminating gametocytes with better outcomes than ASAQ [186]. In a questionnaire performed in Kenya and Uganda (2011) to study the antimalarial effect of A. annua teas, results showed prosperous outcomes after treating about 3000 cases including 250 children and 54 women in the first trimester of pregnancy with malaria [187].


### 6.5. Antiviral Activity as an Approach for Treating COVID-19 Infection

Recently, transposing of medications already in clinical use is the therapeutic strategy for controlling SARS-CoV-2 (COVID-19) infection [188, 189]. The WHO has recommended A. annua as a promising remedy for the treatment of COVID-19; however efficacy and side effects are not determined yet [190] (www.ClinicalTrials.gov, Identifier: NCT04530617). Moreover, A. annua is one of the ingredients of Jinhua Qinggan granule (one of the remedies suggested in the therapeutic regimen of COVID-19 in China) [191]. Currently, a phase II clinical study is under its way to evaluate the efficacy of A. annua in inhibiting the replication of the SARS-CoV-2 virus in patients with high-risk factors such as diabetes and hypertension (www.ClinicalTrials.gov Identifier: NCT04530617). As well, researchers from Saudi Arabia have established a placebo-controlled trial for evaluating the effect of artesunate in patients with mild symptoms of COVID-19 (www.ClinicalTrials.gov Identifier: NCT04387240).Scientific evidence of this strategy might be due to the promising anti-inflammatory, immunomodulatory, and antiviral properties of the bioactive compounds in differentArtemisia species, either among the preclinical or the clinical levels [4]. For instance, 85 patients with SARS were selected for a clinical study and 62 patients received the consigned treatment combined with the traditional Chinese medicine (TCM) (one of its components is herba Artemisia), while 23 patients were assigned in the control group. Results showed that patients who received the combined TCM regimen daily for 3 weeks showed a significant decrease in the total score of symptoms, as well as improvement of the lung X-rays, hepatic function, quality of life, and total score of mental sentiment factors [192].Recently, it was reported that 1250 medical staff in Tongxu County Hospital take one or more decoctions of TCM daily as well as burnArtemisia argyi H.Lév. & Vaniot in the hospital corridor to cut off the route of transmitting infection, where A. argyi was stated as one of the herbs that can be used for contagion prevention by aromatherapy [193]. Artemisinin and its derivatives proved to have promising activities as antiviral agents. For instance, artesunate (100 mg/day) was examined for its efficacy to treat a 12-year patient with human cytomegalovirus (HCMV) infection who exhibited resistance against assigned antiviral drugs (foscarnet and ganciclovir) after stem cell transplantation. Results showed a significant reduction in viral load at day 7, with a virus half-life of 0.9–1.9 days, representing an effective stopover in viral replication [194].


## 6.1. Anti-inflammatory Activity

In a randomized double-blind clinical trial, oral treatments of 42 patients by Arthrem (supercritical CO2-extracted A. annua) at doses 150 mg and 300 mg or placebo twice daily for 12 weeks were tested for their efficacy on stiffness, pain, and functional limitations in osteoarthritis of the hip and knees. Results showed a significant decrease in visual analogue scale (VAS) score and improvement in WOMAC (Western Ontario and McMaster Universities Osteoarthritis) total score only at the low dose of 150 mg [154]. Afterwards, an open-label 6 month extension trial was proceeded to examine the safety of Arthrem in the long run (6 months). Results showed that Arthrem could be a safe and effective agent for osteoarthritis management [155]. In a similar study, 90 patients diagnosed with osteoarthritis applied 3% A. absinthium ointment, 3% A. absinthium liniment, or piroxicam gel (PG) on their knees for 4 weeks. Results showed that A. absinthium ointment revealed significant improvement in all parameters except for WTSS (total stiffness score), where A. absinthium liniment only reduced VAS and WTPS (total pain score) in week 4 with recurrence in week 6 when compared to PG that improved all the parameters with no recurrence [156]. Furthermore, in a randomized controlled clinical trial, 10 patients with Crohn’s disease administrated dried A. absinthium powder (750 mg three times daily) along with their basic therapy for 6 weeks, where another ten patients served as the control group. Results showed a significant reduction in serum TNF-α level and Crohn’s disease activity index (CDAI) scores as well as remission of symptoms in eight patients [157].In a further clinical study, a nasal spray containing an extract ofA. abrotanum mainly composed of EO (4 mg/mL) and flavonols (2.5 μg/mL) was established for treating 12 patients with allergic rhinitis. The EO fraction is composed of 1,8-cineole, linalool, and davanone, while the flavonol fraction contained centauredin, casticin, and quercetin dimethyl-ethers, which are well known for their anti-inflammatory effect. Most of the patients exhibited a significant reduction in nasal congestion, sneezing, and rhinorrhea as well as relief of eye symptoms when compared to the effect of anti-histamines [158].In the sight of cosmetics, twenty-five sensitive skin patients were selected for investigating the efficacy ofA. annua extract on skin reliving. Results showed that consuming cosmetics having A. annua extract for 4 weeks could improve the hydration degree of the cheek cuticle by 63.90%, reduce the transepidermal water loss by 21.51%, reduce the sensitized area by 77.47%, repair skin damage, and reduce redness [159].


## 6.2. Anticancer Activity

Interestingly, artemisinin and its derivatives were reported as potent antitumor compounds with high selectivity on cancer cells without any side effects on normal cells [160]. Their mechanism of action is based on the cleavage of their endoperoxide bridge by Fe2+ in cancerous cells and the production of ROS involved in apoptosis, DNA damage, autophagy, and cell cycle arrest G0/G1 of the cancerous cells. Additionally, they can suppress angiogenesis by inhibiting the secretion of vascular endothelial growth factor (VEGF), vascular endothelial growth factor receptor 2 (VEGFR2), and kinase insert domain receptor (KDR)/flk-1 in tumors, as well as affecting different signaling pathways and transcription factors related to tumor growth [161, 162]. Besides the antitumor effect of artemisinin and its derivatives against human cancer cell lines in vitro [160, 163, 164], different clinical studies were established to ensure their potency. For instance, in a pilot clinical trial at phase II, the anti-tumor effect of dihydroartemisinin (200 mg/day) was tested against advanced cervical carcinoma for 3 weeks in ten women. Results showed a significant reduction in vaginal discharge and pain, with no sign of severe toxicity, as well as improvement in overall signs and symptoms. These patients also exhibited a lower expression of epidermal growth factor receptor (EGFR) and Ki-67 oncogenes [165].Further, in a randomized, controlled clinical trial, artesunate (120 mg, once daily, IV) was combined with vinorelbine (25 mg/m2, once daily, IV) and cisplatin (25 mg/m2, once daily, IV) to treat patients with the advanced lung cancer stage. Results showed significant improvement in the survival rate and hindering of the progression time of the cancerous cell, devoid of extra side effects [166]. In a case report conducted by Singh and Verma [167], artesunate (50 mg) proved to reduce the tumor size of the larynx of a patient with stage II cancer by 70% following 2 weeks of treatment [167]. As well in another case report conducted by Berger et al. [168], the combination of artesunate (50 mg twice/day) with standard chemotherapy showed a significant reduction in death risk and stabilization of the disease case in 2 patients with metastatic uveal melanoma stage IV, when compared to the chemotherapy alone [168]. Furthermore, artemether (40 mg once/day) proved to exhibit a significant improvement in the computed tomography scan of a 75-year-old patient with pituitary macroadenoma. Results showed a decrease in the tumor’s density as well as an improvement in the overall clinical signs and symptoms [169].


## 6.3. Antidiabetic Activity

In a randomized double-blind clinical study, 24 patients with impaired glucose tolerance (IGT) administratedA. dracunculus (1000 mg) or placebo before breakfast and dinner for 90 days. Results showed a significant decrease in systolic blood pressure, glycated hemoglobin, and total insulin secretion as well as a significant increase in the high-density lipoprotein cholesterol level [170].In a dose-response clinical study, ethanolic extract ofArtemisia princeps Pamp. containing eupatilin and jaseocidin was investigated for its antidiabetic effect in 81 patients with hyperglycemia. Patients were randomized into four groups: negative control (lactose 2000 mg/day), positive control (pinitol 1140 mg/day), low-dose extract (2000 mg/day), and high-dose extract (4000 mg/day). Both doses significantly reduced glycated hemoglobin level, where the free fatty acid level in plasma was only lowered in patients administrated the high dose of the extract [171]. In a similar clinical trial, A. absinthium capsules (1 g/twice daily for 30 days) or placebo were administrated to 16 patients with type II diabetes. Results showed that A. absinthium reduced the glucose level by 32%, triglycerides by 10%, total cholesterol by 5%, and LDL level by 6% [172].


## 6.4. Antimalarial Activity

Malaria is considered the most common tropical disease that is provoked by certain parasites of the genusPlasmodium such as P. malariae, P. viva, P. falciparum, and P. ovale [173]. Artemisia species are famous for their content of sesquiterpene lactones that are responsible for the high therapeutic potential of the genus [173, 174]. For instance, artemisinin and its derivatives are the most common sesquiterpene lactones among the genus. Dihydroartemisinin (the active form inside the biological systems) is produced by reducing the lactone of artemisinin. While, alkylation of the hemiacetal group yields arteether and artemether, where artesunate is produced by acylation of the hemiacetal group with succinic acid [175]. Inside in vivo systems, all these derivatives are converted to dihydroartemisinin, where it possesses the highest activity, oral bioavailability, and tolerability with minimal side effects. These compounds are well known for their powerful activity against different species of Plasmodium as they contain the 1,2,4-trioxane moiety that may be responsible for the mechanism of action of the drugs [122]. The antimalarial activity of these compounds outcomes from the presence of Fe2+ after the Plasmodium hemolysis. This Fe2+ is utilized as a catalyst to open the peroxide bridge of the compound, leading to the formation of free radicals, alkylating Plasmodium proteins, and finally causing parasite death. Artemisinin also can perform its antimalarial activity by inhibiting PfATP6, an enzyme for the delivery of Ca2+ into vesicles of the parasite, which is critical for its development [122]. In a cluster-randomized clinical trial performed in different African countries, it was determined that rectal artesunate could take from 4 to 6 h to reduce parasite load, progression of the disease, and risk of death, so it can be a good choice for patients who cannot be treated orally [176]. Moreover, the WHO reviewed different clinical trials performed by the African Quinine Artesunate Malaria Trial multicentre on 5400 children under the age of 15 years with multidrug-resistant severe malaria and suggested IV artesunate (2.4 mg/kg once daily) as a choice to treat malaria [177].A meta-analysis study using single-patient data from different randomized, controlled trials was conducted to compare artemether and quinine in treating severeP. falciparum infection. Results showed that the death rate was significantly reduced with patients treated with artemether (14%) when compared to patients treated with quinine (17%). On the other hand, there was no difference between the 2 treatments in coma recovery, fever clearance, or the progress of neurological toxicity. However, the overall adverse outcomes of either death or neurological toxicity were significantly fewer in the artemether-treated group [178].In a double-blind, randomized, placebo-controlled clinical trial, artesunate was combined with sulfadoxine-pyrimethamine to test their efficacy in reducing the timing of malaria exposure during the infancy stage. Results showed that innate cells produced a balanced level of proinflammatory and regulatory cytokines around 2 years of age, which was accompanied by a lower risk of clinical malaria [179]. Further, it was reported that patients with uncomplicated malaria who administrated this combination therapy exhibited an 84.1% cure rate [180].Despite the potency of artesunate as an antimalarial drug, there are also two main antimalarial regimen options: dihydroartemisinin–piperaquine (DHA–PPQ) and artemether-lumefantrine (AL), which are considered as the first option for treating uncomplicatedP. falciparum malaria globally [181]. For instance, DHA–PPQ was reported to have high benefits in children with uncomplicated malaria in endemic countries [182]. On the other hand, the AL therapy (marketed as “Coartem”) could exert its antimalarial effect through opposing the erythrocytic stages of the parasite and so reducing the number of parasites. In addition, lumefantrine had a much-extended half-life time when combined with artemether and was supposed to clear residuals of the parasites [183].As malaria causes severe maternal and fetal problems, the Centers for Disease Control and Prevention (CDC) suggested AL therapy for treating pregnant women with uncomplicated malaria in the United States throughout the second and third trimesters of pregnancy, at the same doses assigned for nonpregnant women [184]. In a case report implemented by Daddy et al. [185], dried leaves of A. annua were investigated for their efficacy as an antimalarial agent in patients (with P. falciparum infection) not responding to artemisinin combination therapy (ACT) or artesunate (IV). After oral administration of dried leaves of A. annua at a dose (0.5 g/twice daily/5 days), these patients exhibited a subside in clinical symptoms and the parasites were undetected microscopically [185]. In a large-scale double-blind, randomized clinical trial, the antimalarial effect of A. annua and A. afra infusions (1 L/day of dry leaf/twig infusion for 7 days) was compared to artesunate-amodiaquine (ASAQ) by 957 patients with malaria (with P. falciparum infection). Results showed that patients treated with both Artemisia infusions exhibited trophozoites clearance after 24 h when compared to ASAQ which took up to 14 days. Moreover, fever clearance took up to 48 h for ASAQ, but only 24 h for both Artemisia infusions. From days 14–28, gametocytes were undetectable for patients treated with Artemisia infusions, whereas on day 28, ASAQ-treated patients stayed carriers for gametocytes. These results proved that Artemisia infusions could break the life cycle of malaria by eliminating gametocytes with better outcomes than ASAQ [186]. In a questionnaire performed in Kenya and Uganda (2011) to study the antimalarial effect of A. annua teas, results showed prosperous outcomes after treating about 3000 cases including 250 children and 54 women in the first trimester of pregnancy with malaria [187].


## 6.5. Antiviral Activity as an Approach for Treating COVID-19 Infection

Recently, transposing of medications already in clinical use is the therapeutic strategy for controlling SARS-CoV-2 (COVID-19) infection [188, 189]. The WHO has recommended A. annua as a promising remedy for the treatment of COVID-19; however efficacy and side effects are not determined yet [190] (www.ClinicalTrials.gov, Identifier: NCT04530617). Moreover, A. annua is one of the ingredients of Jinhua Qinggan granule (one of the remedies suggested in the therapeutic regimen of COVID-19 in China) [191]. Currently, a phase II clinical study is under its way to evaluate the efficacy of A. annua in inhibiting the replication of the SARS-CoV-2 virus in patients with high-risk factors such as diabetes and hypertension (www.ClinicalTrials.gov Identifier: NCT04530617). As well, researchers from Saudi Arabia have established a placebo-controlled trial for evaluating the effect of artesunate in patients with mild symptoms of COVID-19 (www.ClinicalTrials.gov Identifier: NCT04387240).Scientific evidence of this strategy might be due to the promising anti-inflammatory, immunomodulatory, and antiviral properties of the bioactive compounds in differentArtemisia species, either among the preclinical or the clinical levels [4]. For instance, 85 patients with SARS were selected for a clinical study and 62 patients received the consigned treatment combined with the traditional Chinese medicine (TCM) (one of its components is herba Artemisia), while 23 patients were assigned in the control group. Results showed that patients who received the combined TCM regimen daily for 3 weeks showed a significant decrease in the total score of symptoms, as well as improvement of the lung X-rays, hepatic function, quality of life, and total score of mental sentiment factors [192].Recently, it was reported that 1250 medical staff in Tongxu County Hospital take one or more decoctions of TCM daily as well as burnArtemisia argyi H.Lév. & Vaniot in the hospital corridor to cut off the route of transmitting infection, where A. argyi was stated as one of the herbs that can be used for contagion prevention by aromatherapy [193]. Artemisinin and its derivatives proved to have promising activities as antiviral agents. For instance, artesunate (100 mg/day) was examined for its efficacy to treat a 12-year patient with human cytomegalovirus (HCMV) infection who exhibited resistance against assigned antiviral drugs (foscarnet and ganciclovir) after stem cell transplantation. Results showed a significant reduction in viral load at day 7, with a virus half-life of 0.9–1.9 days, representing an effective stopover in viral replication [194].


## 7. Safety Issues of Artemisia Species

Various reports have been published regarding toxicity related to the overdosing in humans with extracts ofArtemisia; A. absinthium (wormwood) was used in the formulation of the absinthe drink and currently has been restricted in most countries because of neurotoxicity [195, 196]; it was reported that few cups of sage tea or wormwood would be essential to reach the suitable daily intakes [195]. Preclinical studies of the toxicity of Artemisia species were examined in many reports and the most important results were summarized in Table 4. Regarding Artemisia spp. toxicity, different parts of Artemisia spicigera K. Koch and Artemisia fragrans Willd. significantly increased the number of MCF7 and HEK293 cell proliferation [126]. Moreover, the toxicological study of Artemisia judaica L. has been studied by Nofal et al. [197] and observed acute and chronic toxicity. Furthermore, Artemisia parviflora Roxb. ex D. Don showed no significant toxic effect on Swiss albino mice [198] as shown in Table 4. In addition, there is only one report on the toxicity of A. vulgaris [199] while different studies of A. annua demonstrated that it is considered safe and nontoxic up to 5000 mg/kg of the extract [200]. Artemether and the closely related compound arteether are hydrophobic derivatives of dihydroartemisinin reported to have neurotoxicity action [201].Table 4
Toxicological studies ofArtemisia species.
Extract/compoundDosesIn vitro/in vivoRoute of administrationModelEffectRefA. annua /leaf/hexane extract1000, 2000, 2500 mg/kgIn vivoi.p.Wistar albino rats↓carbohydrate, protein, lipid metabolisms, unfavourable effect on nutritional benefits, ↓hematological parameters, ↓toxicity when used acutely in rats[202]A. annua/hydroethanolic extract300, 2000, 5000 mg/kgIn vivoOrallySwiss albino miceNo toxic or lethal reactions of all the doses[200]A. parviflora/aerial parts/ethanolic extract0.10, 0.50, 1.0 g/kgIn vivoOrallySwiss albino miceNo significant toxic effectLD50>1g/kg BW[198]A. abyssinica,A. inculta/aerial parts/ethanolic extracts500 mg/kg, 1 and 3 g/kgIn vivoOrallySwiss albino miceA. inculta, dose=3g/kg: CNS stimulation, A. abyssinica, dose=3g/kg: ↓locomotor activity[203]A. vulgaris/oils—In vivo—Brine shrimpArtemia sp. (larvae)LC50=10.4–23.3 μg/mL germacrene D, camphor, 1,8-cineol, davanone: ↑toxicity[199]A. afra/aqueous extract1.5–5.5 g/kg i.t., 2–24 g/kg o.p.In vivoi.p., orallyBALB/C mice, Wistar ratsNontoxic when given acutely, low chronic toxicity, hepatoprotective effect in high doses[204]Artemether0, 20, 40, 80 mg/kg i.m., 0, 50, 150, 600 mg/kg p.o.In vivoi.m., orallyBeagle dogsHigh i.m. doses: neurological damage,dose=20mg/kg: minimal effects occurred[201]Artemether, artesunate30–100 mg/kg/dayIn vivoi.m.Swiss albino miceArtemether neurotoxicity is significantly more neurotoxic than i.m. artesunate[205]


## 8. Limitations, Therapeutic Perspectives, and Clinical Gaps

AlthoughArtemisia spp. and its constituents show great potential as functional foods, dietary supplements, and safe medicines, some adverse effects have been described in the literature [206]. A. absinthium, grand wormwood, has been included traditionally as a major component of the highly anise-flavoured alcoholic spirit, “Absinthe,” which was the most popular alcoholic beverage of the late 19th century in Europe [207]. Absinthe was prohibited at the beginning of the 20th century as a consequence of adverse symptoms called absinthism [208]. Absinthism symptoms included hallucinations, blindness, mental deterioration, and convulsions. The prebanned Absinthe was probably related to chronic alcoholism [207]. Several drugs can interact with the effects produced by Artemisinin, and therefore, the specialist should be consulted before taking it. A. absinthium is permitted nowadays in foods and alcoholic beverages. The consumption of thujone from Artemisia must not exceed 10 mg/kg according to the European Food Safety Authority (EFSA) and 3 mg/day/person according to European Medicines Agency (EMA) [206].SomeArtemisia species are used in regulating fertility and thus should be avoided in pregnancy due to the possible risk of embryotoxicity at higher doses. For instance, the consumption of A. herba-alba to pregnant mice significantly decreased the fertility ratio of offspring mice [209]. Additionally, consumption of A. kopetdaghensis “Krasch., Popov & Lincz. ex Poljakov” hydroalcoholic extract in pregnancy increases the risk of abortion [210].Skin contact dermatitis has been reported upon exposure to differentArtemisia species [211, 212]. Skin prick testing showed that the majority of patients with allergic rhinitis and asthma have positive reactions to A. vulgaris. Therefore, patients with Compositae sensitization are routinely warned against exposure to Artemisia species [213]. Artemisia-induced dermatitis is attributed to the content of sesquiterpene lactones [214].Pollens fromArtemisia species can cause serious pollinosis [206]. Nasal challenge and bronchial provocation tests verified that pollens, leaves, and stems from Artemisia are serious allergens causing allergic rhinitis and/or asthma [215, 216]. For instance, pollens from mugwort, A. vulgaris, contained allergenic substances such as profiling as well as other crossreactive allergens with immunoglobulin E (IgE) reactivity causing immediate type I allergic reactions [217]. Type I hypersensitivity involves mast cell degranulation and the release of inflammatory mediators such as histamine, causing allergic reactions such as anaphylactic shock [218]. Additionally, A. vulgaris pollens showed the highest levels of endotoxin among other collected plants across 100 locations in Europe [219]. Pollen extracts of six different Artemisia species, A. annua, A. scoparia, A. vulgaris, A. princeps, Artemisia campestris L., and Artemisia tridentata Nutt. exhibited an extensive degree of similarity and crossreactivity [220]. This study also showed that Korean and Norwegian patient sera had the same pattern of reactivity towards A. vulgaris and A. princeps [220].Clinically, administration of the sesquiterpene lactone artemisinin as well as its derivatives such as arteether, artesunate, and artemether in appropriate therapeutic doses for short periods did not show serious side effects [221, 222]. In the liver, artemisinin is converted to various inactive metabolites, such as deoxy artemisinin, deoxyhydroartemisinin, crystal 7, and 9,10-dihydrodeoxyarthemisinin. The reaction is catalyzed by a CYP2B6 enzyme, while another CYP3A4 enzyme acts as a secondary catalyst. These enzymes belong to the cytochrome P450 group present in the smooth endoplasmic reticulum. Artemisinin derivatives are metabolized differently. They are first converted to dihydroartemisinin (DHA). DHA itself is a powerful antimalarial molecule and is active in the bloodstream for two to three hours [223]. The antimalarial activity of artesunate is actually only through DHA [224]. Artesunate is converted to DHA within one minute of absorption. About 90% of all DHA is normally bound to blood plasma. In the liver, the cytochrome P450 enzyme system (including CYP2A6, CYP3A4, and CYP3A5) converts DHA into inactive metabolites [225]. All metabolites are subject to glucuronidation and are excreted in the urine or faeces. UDP-glucuronosyltransferases, especially UGT1A9 and UGT2B7, are responsible for the process [226]. DHA is also excreted in the bile as minor glucuronides, such as tetrahydrofuran acetate. Due to its rapid metabolism, artemisinin is a relatively safe bioactive compound [227]. Because artemisinin interactions with other drugs are not fully known, future clinical studies are needed to establish their potential interaction mechanisms.Other limitations derive from the amounts of active ingredients in plants, depending on the area of cultivation and climate [228–230]. Also, the absorption of bioactive compounds rapidly and in variable quantities requires the future development of pharmaceutical nanoformulations that will improve the bioavailability and implicitly an increased therapeutic efficacy [231, 232].


## 9. Overall Conclusion

Artemisia spp. have been traditionally used for pharmacological purposes and as an edible plant used in food, spices, and beverages. A. annua and A. absinthium are the most famous Artemisia species. This genus distributed worldwide presents diverse chemical constituents mainly EOs and polyphenols. These species contain sesquiterpene lactones that are largely responsible for the therapeutical potential of Artemisia genus. The most studied biological activities of this genus are antioxidant, anti-inflammatory, antitumor, antidiabetic, antimalarial, neuroprotective, and hepatoprotective activities through preclinical and clinical evidence. Artemisia spp. and their constituents show great potential as dietary supplements, functional foods, and safe medicines as antimalarial, antioxidant, anticancer, antinociceptive, anti-inflammatory, and antiviral agents. The antiviral activity for treating COVID-19 infection is a hope for the current pandemic. However, it is really important and necessary for further research investigations for discovering safer Artemisia plant-derived drugs for curing several kinds of diseases.



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