# KIF20B Promotes Cell Proliferation and May Be a Potential Therapeutic Target in Pancreatic Cancer

Authors: Jing Chen; Cui-Cui Zhao; Fei-Ran Chen; Guo-Wei Feng; Fei Luo; Tao Jiang
Journal: Journal of Oncology
 (2021)

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## Abstract

KIFs have been reported to play a critical role in a variety of tumors, and KIF20B is a protein in KFIs. In this research, KIF20B was highly expressed in the GEO database and our hospital’s data, and high expression of KIF20B suggested poor prognosis. We detect the expression of KIF20B in pancreatic cancer and adjacent normal tissues using immunohistochemistry. Knockdown of KIF20B in pancreatic cancer cell lines, PANC-1 and BxPC-3 cells, inhibited cell proliferation which are detected by colony formation assays, CCK8, and western bolt of Ki-67 and PCNA. Xenograft assay showed a similar result in vivo. KIF20B is a potential therapeutic target in pancreatic cancer.


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## Body

## 1. Introduction

Pancreatic cancer is a highly fatal disease in which mortality is closely related to the incidence [1]. Most pancreatic cancer patients remain asymptomatic until the disease is advanced. So, pancreatic cancer is one of the leading causes of fatal disease in cancer across the world [2]. The 5-year survival of patients with complete resection is only 25% [1]. The main types of pancreatic cancer are adenocarcinoma (85%) and endocrine carcinoma (less than 5%) [2–4]. According to the cancer statistics in 2018, about 55,440 people suffered from pancreatic cancer, and pancreatic cancer causes 44,330 deaths [5]. In 2017, there were 53,670 patients with pancreatic cancer and 43,090 patients died because of pancreatic cancer [6]. Pancreatic cancer was 11th common cancer in 2012 [2] but ranked 4th in 2018 of cancer statistics [5]. The five-year survival rate of pancreatic cancer is less than 5% [3]. In the last decades, the number of pancreatic cancer has been increased in both genders [7–9]. Early diagnosis is the key for improving survival.The kinesin superfamily (KIF) proteins are highly conserved proteins with the motor domain, some of which move to the plus end of the microtubule in ATP, which depends on the adenosine triphosphatase activity [10–12]. KIF family plays an important role in many essential biological processes, including mitosis, meiosis, and the transport of macromolecules [13].Among these KIFs, KIF20B was a subset of protein specifically phosphorylated at G2/M transition. KIF20B (previously called Mphosph1 or Mpp1) is a microtubule-associated protein at M-phase which plays a critical role in cytokinesis. Furthermore, KIF20B has been found to play roles in some tumors such as hepatocellular carcinoma [14, 15], bladder cancer [16], colorectal cancer [17], and breast cancer [18]. However, no study has reported the role of KIF20B in pancreatic cancer.In GEO database and our hospital data, the conclusion was found that the patients with high expression of KIF20B had a poor prognosis. Motivated by a desire to understand the effect of KIF20B in pancreatic cancer, KIF20B was knockdown in pancreatic cancer cell lines. Cell proliferation was decreased during the knockdown of KIF20B in vitro and in vivo.


## 2. Materials and Methods

### 2.1. Patients and Samples

Pancreatic tumor samples and adjacent normal tissues were collected from 90 patients in Tianjin Medical University Cancer Institute and Hospital between 2014 and 2017. The adjacent normal tissues were obtained from the margin of pancreatic tumor, >2 cm. All samples were checked by pathologists as pancreatic tumors or normal tissues. The information of patients was approved by patients. The size of the original (primary) tumor (T), nearby (regional) lymph nodes (N), and distant metastasis (M) was classified according to UICC TNM Classification (8th ed.) 2016. After surgery, samples were immediately were fixed in 10% buffered formalin or stored at −80°C until used. This research was approved by our hospital.


### 2.2. Antibodies

Antibodies against KIF20B (western blot 1 : 1000 dilution, immunohistochemistry 1 : 200 dilution, ab122165, Abcam, Cambridge, UK), Ki-67 (1 : 1000 dilution, ab15580, Abcam, Cambridge, UK), PCNA (1 : 1000 dilution, # 13-3900, Invitrogen, Carlsbad,USA), GAPDH (1 : 2000 dilution, KM9001T, Sungen Biotech, Tianjin, China), HRP AffiniPure Goat Anti-Rabbit IgG (1 : 10000 dilution, A21020, AmyJet Scientific, Wuhan, China), HRP AffiniPure Goat Anti-Mouse IgG (1 : 10000 dilution, A0216, AmyJet Scientific, Wuhan, China).


### 2.3. Immunohistochemistry

The antigen of sections was retrieved by citric acid buffer in a microwave for 20 minutes and cool down to temperature. Then, immunohistochemistry was detected according to the instructions of kit (ZsBiO, SPN-9002, Beijing, China). The sections were blocked by hydrogen peroxide and serum and then incubated with primary antibodies for one night at 4°C. Sections were washed three times using PBS, then incubated with the second antibody, and stained with DAB. The results were collected under a microscope (Olympus, Tokyo, Japan). The scores were marked as follows: no staining, 0; slight, 1; moderate, 2; and strong, 3. The distribution of positive staining was scored for five groups: no staining, 0; <25%, 1; 26–50%, 2; 51–75%, 3; and 75–100%, 4. The final scores were calculated by multiplying the proportion and intensity. High-expression was considered as grades ≥4, and 0–3 was low-expression [16].


### 2.4. Cell Lines and Cell Culture

The human pancreatic cancer cell lines, PANC-1 [17] and BxPC-3 [18], were purchased from Cell Center of Chinese Academy of Medical Sciences (Shanghai, China). All cell lines were authenticated by STR profiling and checked for the absence of mycoplasma. Both cell lines were grew in PRMI 1640 (61870-036, Gibco, Waltham, Massachusetts, USA) supplemented with 10% fetal bovine serum (10099-141–FBS, Gibco, Waltham, Massachusetts, USA) and 1% penicillin-streptomycin solution (P1410, Solarbio, Beijing, China) in 5% CO2 at 37°C.


### 2.5. KIF20B Knockdown and Stable Cell Lines

A lentivirus plasmid, pll3.7, with insertion of shRNA for human KIF20B knockdown was constructed. An empty pll3.7 plasmid was used as a control plasmid. The lentivirus was generated by 293T after transfection corresponding lentivirus plasmids by lipofectamine 3000 (L3000015, Thermo, Waltham, Massachusetts, USA). PANC-1 and BxPC-3 cells were infected with shKIF20B lentivirus in the presence of Polybrene (5μg/ml, TR-1003, Sigma, St. Louis, Missouri, USA) for 36 hours. After that, cells were cultured with puromycin (5 μg/ml, P8230, Solarbio, Beijing, China) to select positive cells until stable cell lines are formed. The shRNA for KIF20B, AATCAGATTCATTGATTCAAGAG (Cat# SH836784, Vigene Biosciences Inc, Maryland, USA), was purchased from Vigene.


### 2.6. Western Blot

For protein from cells, cells were washed with cold PBS three times and subsequently 200μl RIPR containing 2 μl protease inhibitors was added into 6 wells for 30 min at 4°C. For protein from tissues, fresh tissues were washed with cold PBS and RIPR was added to tissues in EP tubes. Then, EP tubes were moved to high throughput homogenization (Scientz, Ningbo, Zhejiang, China) for 1 min in 60 Hz. Lysates were centrifuged at 12000 rpm/min for 30 min at 4°C, and then the supernatant was moved to new Eppendorf tubes and stored at −20°C until used. 5 μg protein and 5x SDS-PAGE loading buffer (93-2108-10, MultiSciences, Hangzhou, Zhajiang, China) were added into each EP tubes to 1x, heated for 5 min at 95°C. Protein was separated on 10% SDS-PAGE (P1200, Solarbio, Beijing, China) gels and then transferred into PVDF membranes (FFP19, Beyotime, Shanghai, China). Membranes were washed with TBST for 15 minutes at room temperature. PVDF membranes were blocked with 10% milk for 1 hour at room temperature. After washed, PVDF was incubated with primary antibody overnight at 4°C in shaking table. Then, membranes were washed for 30 minutes with TBST and incubated appropriate HRP-conjugated secondary antibody for 1 hour at room temperature. Proteins were detected with Thermo Scientific Pierce ECL (32106, Thermo, Waltham, Massachusetts, USA) after washing three times for 10 minutes.


### 2.7. Extraction of Total RNA and QPCR

Total RNA from cells or xenograft tumor was isolated using TRIzol reagent (15596026, Invitrogen, Carlsbad, USA). 0.5 ml TRIzol was added per 105–107 cells to lyse the cells, and then 0.5 ml isopropanol was added to the aqueous phase, incubated for 10 minutes, and centrifuged for 10 minutes at 12,000g at 4°C. The supernatant was discarded, and 1ml 75% ethanol was added to wash pellet and centrifuged for 5 minutes at 7500 g at 4°C. Resuspend the RNA in 30 μl RNase-free water after drying the RNA pellet for 5 minutes. For cDNA reverse transcription using RevertAid First Strand cDNA Synthesis Kit (K1621, Thermo, Waltham, Massachusetts, USA), qRT-PCR was analyzed using GoTaq qPCR Master Mix (A6001, Promega, Madison, Wisconsin, USA). Data were calculated using CT values of all samples and standards based on housekeeping gene GAPDH. The relative expression of KIF20B was analyzed using the 2−ΔΔCT method [19]. Primers for KIF20B and GAPDH are listed as follows.KIF20B, forward primer: 5′TGCTGAAAGACCCTCAAAGCATCCT-3′, reverse primer: 5′ACTGGACTGGTCACAACTGTTCACG [20]-3′GAPDH, forward primer: 5′-GGT GGT CTC CTC TGA CTT CAA CA-3′, reverse primer: 5′-GTT GCT GTA GCC AAA TTC GTT GT-3′ [21]


### 2.8. Cell Colony Formation Assay

1,000 cells were seeded into 6 wells with PRMI 1640 + 10% FBS and incubated in 5% CO2 at 37°C for 14 days. The complete culture medium was changed every 3 days. Cells were fixed with 4% paraformaldehyde for 30 minutes and stained with methylene blue after washing three times using PBS. The results were photographed and counted using ImageJ software.


### 2.9. CCK8 Assay for Cell Proliferation

PANC-1, BxPC-3, and knockdown KIF20B cells were collected at log-growth phase and seeded into 96 wells, 3000 cells/well, with 100μl PRMI 1640 containing 10% FBS, 1% penicillin-streptomycin solution in 5% CO2 at 37°C. After 48 h, 10 μl CCK8 solution (96002, Sigma, St. Louis, Missouri, USA) was added into each well and incubated 4 hours with cells in 5% CO2 at 37°C. Then, absorbance values (OD) were collected using a microplate reader. All experiments were repeated three times.


### 2.10. Flow Cytometry

Cell cycle assay was performed according the manufacturer’s instructions. In brief, cells were fixed with 70% ethanol overnight, and then cells were resuspended in 500μl FACS buffer with 100μg/ml RNase A, 50μg/ml PI. Then, the data were analyzed by ModFit software. For cell apoptosis, 106 living cells were stained by FITC Annexin V Apoptosis Detection Kit with PI (640914, Biolegend, San Diego, California, USA) in 500μl FACS buffer and analyzed by BD FC500 flow cytometry.


### 2.11. Migration Assay

5 × 104 cells were seeded into the top chambers of transwell plates with FBS-free PRMI-1640, and 600μl complete medium was added into the lower chambers. Cells were incubated for 36h, fixed with 75% ethanol, and stained with crystal violet.


### 2.12. Wound Healing Assay

20 × 104 cells were seeded into 6 wells and incubated for 24 h. Then, the cells were scratched with 100μl pipette tip, and dead cells were removed with PBS. After 48 h, cells were fixed and stained with crystal violet.


### 2.13. In Vivo Experiments

All mouse research studies were approved by our hospital. Nude BalB/c mice (6–8 weeks, 18–22 g) were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China). For xenografted tumor model, 5 × 106 PANC-1 cells or knockdown KIF20B were collected in 150 μl PBS and injected into BABL/c nude mice. After tumor formation (day 14), the tumor length and width were measured every 3 days, and the volume of tumor was calculated using V = 0.5 × length × width2 [22]. All mice were sacrificed 29 days after injection and removed to photograph. Xenografted tumors were fixed using 4% formaldehyde for immunohistochemistry assays.


### 2.14. Statistical Analysis

Data were analyzed with SPSS 22.0 software (SPSS Inc, IBM Corp, Armonk, NY). For the immunohistochemistry experiments, associations between KIF20B expression and the clinicopathological features were evaluated using chi2 tests. Associations of survival and tumor progression and KIF20B expression were estimated by Kaplan–Meier method and log-rank tests. Data are shown as the mean ± standard deviation (SD) in vitro and in vivo experiments on PANC-1 and BxPC-3 cells, and student’s t-test was used for statistical comparisons. A value of P<0.05 was set to be statistically significant.


## 2.1. Patients and Samples

Pancreatic tumor samples and adjacent normal tissues were collected from 90 patients in Tianjin Medical University Cancer Institute and Hospital between 2014 and 2017. The adjacent normal tissues were obtained from the margin of pancreatic tumor, >2 cm. All samples were checked by pathologists as pancreatic tumors or normal tissues. The information of patients was approved by patients. The size of the original (primary) tumor (T), nearby (regional) lymph nodes (N), and distant metastasis (M) was classified according to UICC TNM Classification (8th ed.) 2016. After surgery, samples were immediately were fixed in 10% buffered formalin or stored at −80°C until used. This research was approved by our hospital.


## 2.2. Antibodies

Antibodies against KIF20B (western blot 1 : 1000 dilution, immunohistochemistry 1 : 200 dilution, ab122165, Abcam, Cambridge, UK), Ki-67 (1 : 1000 dilution, ab15580, Abcam, Cambridge, UK), PCNA (1 : 1000 dilution, # 13-3900, Invitrogen, Carlsbad,USA), GAPDH (1 : 2000 dilution, KM9001T, Sungen Biotech, Tianjin, China), HRP AffiniPure Goat Anti-Rabbit IgG (1 : 10000 dilution, A21020, AmyJet Scientific, Wuhan, China), HRP AffiniPure Goat Anti-Mouse IgG (1 : 10000 dilution, A0216, AmyJet Scientific, Wuhan, China).


## 2.3. Immunohistochemistry

The antigen of sections was retrieved by citric acid buffer in a microwave for 20 minutes and cool down to temperature. Then, immunohistochemistry was detected according to the instructions of kit (ZsBiO, SPN-9002, Beijing, China). The sections were blocked by hydrogen peroxide and serum and then incubated with primary antibodies for one night at 4°C. Sections were washed three times using PBS, then incubated with the second antibody, and stained with DAB. The results were collected under a microscope (Olympus, Tokyo, Japan). The scores were marked as follows: no staining, 0; slight, 1; moderate, 2; and strong, 3. The distribution of positive staining was scored for five groups: no staining, 0; <25%, 1; 26–50%, 2; 51–75%, 3; and 75–100%, 4. The final scores were calculated by multiplying the proportion and intensity. High-expression was considered as grades ≥4, and 0–3 was low-expression [16].


## 2.4. Cell Lines and Cell Culture

The human pancreatic cancer cell lines, PANC-1 [17] and BxPC-3 [18], were purchased from Cell Center of Chinese Academy of Medical Sciences (Shanghai, China). All cell lines were authenticated by STR profiling and checked for the absence of mycoplasma. Both cell lines were grew in PRMI 1640 (61870-036, Gibco, Waltham, Massachusetts, USA) supplemented with 10% fetal bovine serum (10099-141–FBS, Gibco, Waltham, Massachusetts, USA) and 1% penicillin-streptomycin solution (P1410, Solarbio, Beijing, China) in 5% CO2 at 37°C.


## 2.5. KIF20B Knockdown and Stable Cell Lines

A lentivirus plasmid, pll3.7, with insertion of shRNA for human KIF20B knockdown was constructed. An empty pll3.7 plasmid was used as a control plasmid. The lentivirus was generated by 293T after transfection corresponding lentivirus plasmids by lipofectamine 3000 (L3000015, Thermo, Waltham, Massachusetts, USA). PANC-1 and BxPC-3 cells were infected with shKIF20B lentivirus in the presence of Polybrene (5μg/ml, TR-1003, Sigma, St. Louis, Missouri, USA) for 36 hours. After that, cells were cultured with puromycin (5 μg/ml, P8230, Solarbio, Beijing, China) to select positive cells until stable cell lines are formed. The shRNA for KIF20B, AATCAGATTCATTGATTCAAGAG (Cat# SH836784, Vigene Biosciences Inc, Maryland, USA), was purchased from Vigene.


## 2.6. Western Blot

For protein from cells, cells were washed with cold PBS three times and subsequently 200μl RIPR containing 2 μl protease inhibitors was added into 6 wells for 30 min at 4°C. For protein from tissues, fresh tissues were washed with cold PBS and RIPR was added to tissues in EP tubes. Then, EP tubes were moved to high throughput homogenization (Scientz, Ningbo, Zhejiang, China) for 1 min in 60 Hz. Lysates were centrifuged at 12000 rpm/min for 30 min at 4°C, and then the supernatant was moved to new Eppendorf tubes and stored at −20°C until used. 5 μg protein and 5x SDS-PAGE loading buffer (93-2108-10, MultiSciences, Hangzhou, Zhajiang, China) were added into each EP tubes to 1x, heated for 5 min at 95°C. Protein was separated on 10% SDS-PAGE (P1200, Solarbio, Beijing, China) gels and then transferred into PVDF membranes (FFP19, Beyotime, Shanghai, China). Membranes were washed with TBST for 15 minutes at room temperature. PVDF membranes were blocked with 10% milk for 1 hour at room temperature. After washed, PVDF was incubated with primary antibody overnight at 4°C in shaking table. Then, membranes were washed for 30 minutes with TBST and incubated appropriate HRP-conjugated secondary antibody for 1 hour at room temperature. Proteins were detected with Thermo Scientific Pierce ECL (32106, Thermo, Waltham, Massachusetts, USA) after washing three times for 10 minutes.


## 2.7. Extraction of Total RNA and QPCR

Total RNA from cells or xenograft tumor was isolated using TRIzol reagent (15596026, Invitrogen, Carlsbad, USA). 0.5 ml TRIzol was added per 105–107 cells to lyse the cells, and then 0.5 ml isopropanol was added to the aqueous phase, incubated for 10 minutes, and centrifuged for 10 minutes at 12,000g at 4°C. The supernatant was discarded, and 1ml 75% ethanol was added to wash pellet and centrifuged for 5 minutes at 7500 g at 4°C. Resuspend the RNA in 30 μl RNase-free water after drying the RNA pellet for 5 minutes. For cDNA reverse transcription using RevertAid First Strand cDNA Synthesis Kit (K1621, Thermo, Waltham, Massachusetts, USA), qRT-PCR was analyzed using GoTaq qPCR Master Mix (A6001, Promega, Madison, Wisconsin, USA). Data were calculated using CT values of all samples and standards based on housekeeping gene GAPDH. The relative expression of KIF20B was analyzed using the 2−ΔΔCT method [19]. Primers for KIF20B and GAPDH are listed as follows.KIF20B, forward primer: 5′TGCTGAAAGACCCTCAAAGCATCCT-3′, reverse primer: 5′ACTGGACTGGTCACAACTGTTCACG [20]-3′GAPDH, forward primer: 5′-GGT GGT CTC CTC TGA CTT CAA CA-3′, reverse primer: 5′-GTT GCT GTA GCC AAA TTC GTT GT-3′ [21]


## 2.8. Cell Colony Formation Assay

1,000 cells were seeded into 6 wells with PRMI 1640 + 10% FBS and incubated in 5% CO2 at 37°C for 14 days. The complete culture medium was changed every 3 days. Cells were fixed with 4% paraformaldehyde for 30 minutes and stained with methylene blue after washing three times using PBS. The results were photographed and counted using ImageJ software.


## 2.9. CCK8 Assay for Cell Proliferation

PANC-1, BxPC-3, and knockdown KIF20B cells were collected at log-growth phase and seeded into 96 wells, 3000 cells/well, with 100μl PRMI 1640 containing 10% FBS, 1% penicillin-streptomycin solution in 5% CO2 at 37°C. After 48 h, 10 μl CCK8 solution (96002, Sigma, St. Louis, Missouri, USA) was added into each well and incubated 4 hours with cells in 5% CO2 at 37°C. Then, absorbance values (OD) were collected using a microplate reader. All experiments were repeated three times.


## 2.10. Flow Cytometry

Cell cycle assay was performed according the manufacturer’s instructions. In brief, cells were fixed with 70% ethanol overnight, and then cells were resuspended in 500μl FACS buffer with 100μg/ml RNase A, 50μg/ml PI. Then, the data were analyzed by ModFit software. For cell apoptosis, 106 living cells were stained by FITC Annexin V Apoptosis Detection Kit with PI (640914, Biolegend, San Diego, California, USA) in 500μl FACS buffer and analyzed by BD FC500 flow cytometry.


## 2.11. Migration Assay

5 × 104 cells were seeded into the top chambers of transwell plates with FBS-free PRMI-1640, and 600μl complete medium was added into the lower chambers. Cells were incubated for 36h, fixed with 75% ethanol, and stained with crystal violet.


## 2.12. Wound Healing Assay

20 × 104 cells were seeded into 6 wells and incubated for 24 h. Then, the cells were scratched with 100μl pipette tip, and dead cells were removed with PBS. After 48 h, cells were fixed and stained with crystal violet.


## 2.13. In Vivo Experiments

All mouse research studies were approved by our hospital. Nude BalB/c mice (6–8 weeks, 18–22 g) were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China). For xenografted tumor model, 5 × 106 PANC-1 cells or knockdown KIF20B were collected in 150 μl PBS and injected into BABL/c nude mice. After tumor formation (day 14), the tumor length and width were measured every 3 days, and the volume of tumor was calculated using V = 0.5 × length × width2 [22]. All mice were sacrificed 29 days after injection and removed to photograph. Xenografted tumors were fixed using 4% formaldehyde for immunohistochemistry assays.


## 2.14. Statistical Analysis

Data were analyzed with SPSS 22.0 software (SPSS Inc, IBM Corp, Armonk, NY). For the immunohistochemistry experiments, associations between KIF20B expression and the clinicopathological features were evaluated using chi2 tests. Associations of survival and tumor progression and KIF20B expression were estimated by Kaplan–Meier method and log-rank tests. Data are shown as the mean ± standard deviation (SD) in vitro and in vivo experiments on PANC-1 and BxPC-3 cells, and student’s t-test was used for statistical comparisons. A value of P<0.05 was set to be statistically significant.


## 3. Results

### 3.1. High Expression of KIF20B Suggests Poor Prognosis in Pancreatic Cancer

In previous research studies, KIF20B has been found to play an important role in multiple tumors, such as breast cancer, bladder cancer [23, 24], and hepatocellular carcinoma [15]. However, there was not a report of KIF20B expression between pancreatic cancer and adjacent normal tissue. Firstly, the expression of KIF20B and prognosis in pancreatic cancer was analyzed in GEO database. Obviously, high expression of KIF20B was found in pancreatic cancer (n = 179) contrasted with adjacent normal tissues (n = 171, Figure 1(a)). 178 patients with pancreatic adenocarcinoma were divided into two groups (89 patients with high expression of KIF20B and 89 patients with low expression of KIF20B ) in this database. According to this cohort, patients with a high expression of KIF20B suffered low overall survival (OS) and disease-free survival (DFS) (Figures 1(b) and 1(c)). These GEO data suggested that KIF20B had high expression and correlated with poor prognosis.Figure 1
High expression of KIF20B is negatively correlated with prognosis in pancreatic cancer. (a) KIF20B overexpression in pancreatic cancer in GEO database. (b) Disease-free survival from GEO database with different expression levels of KIF20B. (c) Overall survival from GEO database. Student’st test (P∗<0.05).
(a)(b)(c)


### 3.2. High KIF20B Is Positively Correlated with Poor Prognosis in Samples from Our Hospital

To clarify pancreatic cancer in which KIF20B was highly expressed, we assessed the expression of KIF20B in primary pancreatic cancer in our own specimens using immunohistochemistry and collected patient’s information such as, KIF20B expression, age, gender, TNM stage, tumor grade and size, lymph node metastasis, and vascular invasion (Table1). KIF20B has different expression levels in different pancreatic ductal adenocarcinoma specimens (N = 90). We further examined the expression of KIF20B in adjacent normal tissues and found that KIF20B has low expression in normal tissues compared with pancreatic cancer (Figures 2(a) and 2(b)). Combined with patient information, the expression level of KIF20B was higher in high TNM stage, lymph node metastasis, or vascular invasion. KFI20B expression was not correlated with age, gender, tumor grade, and tumor size in our specimens.Table 1
Relationships of KIF20B and clinicopathological characteristics in 90 patients with pancreatic ductal adenocarcinoma.
FeatureAll,n = 90KIF20B expressionχ2PLowHighn = 46n = 44Age (year)2.4010.121<55542430≥55362214Gender1.0760.300Male502822Female401822pTNM stage4.8040.028∗I26188II-III642836Tumor grade2.2770.131Low402416High502228Tumor size2.8230.093<5281810≥5622834Lymph node metastasis5.3590.021∗Yes381424No523220Vascular invasion5.4710.019∗Yes341222No563422Figure 2
KIF20B is highly expressed in samples from hospital. (a) Representative pictures in immunohistochemical staining of KIF20B in pancreatic cancer. (b) Representative pictures in immunohistochemistry of KIF20B in adjacent normal tissues. (c, d) Overall survival rate and relapse-free survival rate in pancreatic cancer patients with high/low KIF20B. Student’st-test (P∗<0.05).
(a)(b)(c)(d)


### 3.3. KIF20B Knockdown via Lentivirus-Mediated shRNA in Different Human Pancreatic Cancer Cell Lines

To further investigate the effect of KIF20B in pancreatic cancer, we knockdown KIF20B via lentivirus-mediated shRNA in human pancreatic cancer cell lines, PANC-1 and BxPC-3. Firstly, we constructed lentivirus shRNA plasmids, PLL3.7-shKIF20B, and then packed lentivirus using 293T cells. KIF20B mRNA expression was detected using QPCR in PANC-1 cells after KIF20B knockdown via lentivirus-mediated shRNA, and the expression level was obviously lower than the control cell. BxPC-3 cell had a similar result (Figure3(a)). Additionally, protein expression level of KIF20B was detected in PANC-1 and BxPC-3 cell lines using western blotting. Consistent with mRNA expression, the protein of KIF20B was decreased (Figure 3(b)).Figure 3
KIF20B knockdown via lentivirus‐mediated shRNA in different human pancreatic cancer cell lines. (a) QPCR was conducted on control cell and knockdown cell for KIF20B mRNA expression in PANC-1 and BxPC-3 cell lines. (b) Protein expression was detected using western blotting in control cell and KIF20B knockdown cell (up: representative image of western blotting; down: quantification of KIF20B). Student’st-test (P∗<0.05).
(a)(b)


### 3.4. KIF20B Knockdown Inhibited Proliferation in Human Pancreatic Cancer Cell Lines

Considering that high expression level of KIF20B was associated with lymph node metastasis and vascular invasion, the effects of KIF20B were reported in other research studies [14, 23, 25]. Through cell clonal formation, the growth rate of knockdown KIF20B significantly decreased compared with control cells (Figure 4(a)). The result was repeated in PANC-1 cell and BxPC-3 cell. In CCK8 cell proliferation assay, cell proliferation in PANC-1 with KIF20B knockdown was lower than sh-control cell in PANC-1 cells. Similar results were obtained from BxPC-3 cells (Figure 4(b)). Ki-67 [26, 27] and PCNA [28, 29] were protein markers for cell proliferation, so we detected the expression level of Ki-67 and PCNA by western blotting to evaluate cell proliferation. Proliferation markers, KI67 and PCNA, were decreased in knockdown KIF20B cell lines (Figures 4(c) and 4(d)). From those data, a conclusion can be obtained that KIF20B promotes cell proliferation in human pancreatic cancer cell lines. To extend our findings that depletion of KIF20B inhibited cell proliferation, cell cycle assay was performed with scramble cells and shKIF20B cells, and the results showed that depletion of KIF20B induced cell cycle arrest in S/G2 (Figure 4(e)). However, knockdown KIF20B does not impair cell migration and cell apoptosis (Supplement Figure 1).Figure 4
The KIF20B knockdown decreased proliferation in human pancreatic cancer cell lines. (a) Representative picture and quantification of clonal formation in control and knockdown cell lines. (b) Cell proliferation was detected using CCK8. (c, d) Cell proliferation markers, Ki-67 and PCNA, and expression level in control cell and KIF20B knockdown cell. (e) Cell cycle in control cell and KIF20B knockdown cell. Student’st-test (P∗<0.05).
(a)(b)(c)(d)(e)


### 3.5. KIF20B Knockdown Inhibits Pancreatic Xenograft Growth In Vivo

In in vitro research studies, we observed that knockdown KIF20B reduced cell proliferation via cell clonal formation and proliferation protein markers. To further evaluate the effects of KIF20B against pancreatic cancer, subcutaneous PANC-1 cell xenograft model was used. 5 × 106 cells were injected subcutaneously into the armpit of mice, and tumor volume was calculated every 3 days after 14 days. These mice were sacrificed in 29 days. As shown in Figure 5(a), the growth of xenograft with KIF20B knockdown is slower than control xenograft. In order to identify the expression level of KIF20B in xenograft, wester blotting was used to detect KIF20B. Xenograft with shKIF20B had low expression level (Figure 5(b)), and the result was similar with immunohistochemistry assay (Figure 5(c)).Figure 5
The KIF20B knockdown inhibits pancreatic xenograft growth in vivo. (a) The tumor growth-curve of tumor volume according to time in control or knockdown groups (left). Representative picture of tumor in 29 days (right).n = 5. (b) KIF20B protein expression in xenograft tumor. (c) KIF20B protein expression was detected using immunohistochemistry. Representative pictures of immunohistochemistry in xenograft tumor (up). Quantification of expression in xenograft tumor (down). Student’s t-test (P∗<0.05).
(a)(b)(c)


## 3.1. High Expression of KIF20B Suggests Poor Prognosis in Pancreatic Cancer

In previous research studies, KIF20B has been found to play an important role in multiple tumors, such as breast cancer, bladder cancer [23, 24], and hepatocellular carcinoma [15]. However, there was not a report of KIF20B expression between pancreatic cancer and adjacent normal tissue. Firstly, the expression of KIF20B and prognosis in pancreatic cancer was analyzed in GEO database. Obviously, high expression of KIF20B was found in pancreatic cancer (n = 179) contrasted with adjacent normal tissues (n = 171, Figure 1(a)). 178 patients with pancreatic adenocarcinoma were divided into two groups (89 patients with high expression of KIF20B and 89 patients with low expression of KIF20B ) in this database. According to this cohort, patients with a high expression of KIF20B suffered low overall survival (OS) and disease-free survival (DFS) (Figures 1(b) and 1(c)). These GEO data suggested that KIF20B had high expression and correlated with poor prognosis.Figure 1
High expression of KIF20B is negatively correlated with prognosis in pancreatic cancer. (a) KIF20B overexpression in pancreatic cancer in GEO database. (b) Disease-free survival from GEO database with different expression levels of KIF20B. (c) Overall survival from GEO database. Student’st test (P∗<0.05).
(a)(b)(c)


## 3.2. High KIF20B Is Positively Correlated with Poor Prognosis in Samples from Our Hospital

To clarify pancreatic cancer in which KIF20B was highly expressed, we assessed the expression of KIF20B in primary pancreatic cancer in our own specimens using immunohistochemistry and collected patient’s information such as, KIF20B expression, age, gender, TNM stage, tumor grade and size, lymph node metastasis, and vascular invasion (Table1). KIF20B has different expression levels in different pancreatic ductal adenocarcinoma specimens (N = 90). We further examined the expression of KIF20B in adjacent normal tissues and found that KIF20B has low expression in normal tissues compared with pancreatic cancer (Figures 2(a) and 2(b)). Combined with patient information, the expression level of KIF20B was higher in high TNM stage, lymph node metastasis, or vascular invasion. KFI20B expression was not correlated with age, gender, tumor grade, and tumor size in our specimens.Table 1
Relationships of KIF20B and clinicopathological characteristics in 90 patients with pancreatic ductal adenocarcinoma.
FeatureAll,n = 90KIF20B expressionχ2PLowHighn = 46n = 44Age (year)2.4010.121<55542430≥55362214Gender1.0760.300Male502822Female401822pTNM stage4.8040.028∗I26188II-III642836Tumor grade2.2770.131Low402416High502228Tumor size2.8230.093<5281810≥5622834Lymph node metastasis5.3590.021∗Yes381424No523220Vascular invasion5.4710.019∗Yes341222No563422Figure 2
KIF20B is highly expressed in samples from hospital. (a) Representative pictures in immunohistochemical staining of KIF20B in pancreatic cancer. (b) Representative pictures in immunohistochemistry of KIF20B in adjacent normal tissues. (c, d) Overall survival rate and relapse-free survival rate in pancreatic cancer patients with high/low KIF20B. Student’st-test (P∗<0.05).
(a)(b)(c)(d)


## 3.3. KIF20B Knockdown via Lentivirus-Mediated shRNA in Different Human Pancreatic Cancer Cell Lines

To further investigate the effect of KIF20B in pancreatic cancer, we knockdown KIF20B via lentivirus-mediated shRNA in human pancreatic cancer cell lines, PANC-1 and BxPC-3. Firstly, we constructed lentivirus shRNA plasmids, PLL3.7-shKIF20B, and then packed lentivirus using 293T cells. KIF20B mRNA expression was detected using QPCR in PANC-1 cells after KIF20B knockdown via lentivirus-mediated shRNA, and the expression level was obviously lower than the control cell. BxPC-3 cell had a similar result (Figure3(a)). Additionally, protein expression level of KIF20B was detected in PANC-1 and BxPC-3 cell lines using western blotting. Consistent with mRNA expression, the protein of KIF20B was decreased (Figure 3(b)).Figure 3
KIF20B knockdown via lentivirus‐mediated shRNA in different human pancreatic cancer cell lines. (a) QPCR was conducted on control cell and knockdown cell for KIF20B mRNA expression in PANC-1 and BxPC-3 cell lines. (b) Protein expression was detected using western blotting in control cell and KIF20B knockdown cell (up: representative image of western blotting; down: quantification of KIF20B). Student’st-test (P∗<0.05).
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## 3.4. KIF20B Knockdown Inhibited Proliferation in Human Pancreatic Cancer Cell Lines

Considering that high expression level of KIF20B was associated with lymph node metastasis and vascular invasion, the effects of KIF20B were reported in other research studies [14, 23, 25]. Through cell clonal formation, the growth rate of knockdown KIF20B significantly decreased compared with control cells (Figure 4(a)). The result was repeated in PANC-1 cell and BxPC-3 cell. In CCK8 cell proliferation assay, cell proliferation in PANC-1 with KIF20B knockdown was lower than sh-control cell in PANC-1 cells. Similar results were obtained from BxPC-3 cells (Figure 4(b)). Ki-67 [26, 27] and PCNA [28, 29] were protein markers for cell proliferation, so we detected the expression level of Ki-67 and PCNA by western blotting to evaluate cell proliferation. Proliferation markers, KI67 and PCNA, were decreased in knockdown KIF20B cell lines (Figures 4(c) and 4(d)). From those data, a conclusion can be obtained that KIF20B promotes cell proliferation in human pancreatic cancer cell lines. To extend our findings that depletion of KIF20B inhibited cell proliferation, cell cycle assay was performed with scramble cells and shKIF20B cells, and the results showed that depletion of KIF20B induced cell cycle arrest in S/G2 (Figure 4(e)). However, knockdown KIF20B does not impair cell migration and cell apoptosis (Supplement Figure 1).Figure 4
The KIF20B knockdown decreased proliferation in human pancreatic cancer cell lines. (a) Representative picture and quantification of clonal formation in control and knockdown cell lines. (b) Cell proliferation was detected using CCK8. (c, d) Cell proliferation markers, Ki-67 and PCNA, and expression level in control cell and KIF20B knockdown cell. (e) Cell cycle in control cell and KIF20B knockdown cell. Student’st-test (P∗<0.05).
(a)(b)(c)(d)(e)


## 3.5. KIF20B Knockdown Inhibits Pancreatic Xenograft Growth In Vivo

In in vitro research studies, we observed that knockdown KIF20B reduced cell proliferation via cell clonal formation and proliferation protein markers. To further evaluate the effects of KIF20B against pancreatic cancer, subcutaneous PANC-1 cell xenograft model was used. 5 × 106 cells were injected subcutaneously into the armpit of mice, and tumor volume was calculated every 3 days after 14 days. These mice were sacrificed in 29 days. As shown in Figure 5(a), the growth of xenograft with KIF20B knockdown is slower than control xenograft. In order to identify the expression level of KIF20B in xenograft, wester blotting was used to detect KIF20B. Xenograft with shKIF20B had low expression level (Figure 5(b)), and the result was similar with immunohistochemistry assay (Figure 5(c)).Figure 5
The KIF20B knockdown inhibits pancreatic xenograft growth in vivo. (a) The tumor growth-curve of tumor volume according to time in control or knockdown groups (left). Representative picture of tumor in 29 days (right).n = 5. (b) KIF20B protein expression in xenograft tumor. (c) KIF20B protein expression was detected using immunohistochemistry. Representative pictures of immunohistochemistry in xenograft tumor (up). Quantification of expression in xenograft tumor (down). Student’s t-test (P∗<0.05).
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## 4. Discussion

Pancreatic cancer is a poor prognosis disease, and five-year survival of which is as low as 2% in some countries, despite the surgical technique is improving [30]. Pancreatic cancer has increased by 1.03% per year in 1973 to 2014 [31], and it is predicted that pancreatic cancer will become the 2nd leading cause of cancer-related deaths in America by 2030 [6, 32]. Pancreatic cancer has caused a serious financial burden on society.In recent years, kinesin as a potential new target for cancer has caused great concern [11, 33]. KIF family was reported to play a critical role in many solid cancers, such as prostate cancer [34], breast cancer [35], and esophageal squamous cell carcinoma [36]. It has been reported that KIF20B was upregulated in human cancer and promoted cell proliferation, but there was no research in pancreatic cancer. Firstly, the GEO database and our data were analyzed, and we found that the expression of KIF20B was negatively correlated with overall survival. In our data, patients with high expression of KIF20B had a higher pTNM state, lymph node metastasis, and vascular invasion (P<0.05). This was similar with Liu et al.’s research that KIF20B was overexpressed in hepatocellular carcinoma and it is essential for proliferation [14]. Similarly, KIF20B was overexpressed in human colorectal cancer and promoted epithelia-mesenchymal transition (EMT) [25].To investigate the role of KIF20B in pancreatic cancer, KIF20B was knockdown in pancreatic cancer cell lines, PANC-1 and BxPC-3 cells. QPCR and western blotting were used for detecting mRNA and protein expression after adding lentivirus-shKIF20B to cells. Then, cell colony formation assay and CCK8 assay detected cell proliferation. Results showed that cell proliferation has a positive correlation with KIF20B expression level. KI67 and PCNA, a protein proliferation marker, assays showed similar results. However, Supplement Figure1 showed that shKIF20B in PANC-1 and BxPC-3 had no effect on cell migration, wound healing, and cell apoptosis.KIFs, including 45 KIFs with varying functions and 14 subfamilies, were discovered in human [37–39]. However, all of them have a highly conserved motor domain that binds to microtubules. KIFs have a common function in chromosomes transport during mitosis [10]. So, misregulation of KIF20B may contribute to tumorigenesis. Knockdown of KIF20B leaded to mitotic arrest, which decreased cell proliferation [24, 40]. Similarly, tumor growth was slower in xenograft with knocking down KIF20B in PANC-1 cells in vivo.In previous research studies, KIF20B can be used as a potential therapeutic target for a variety of tumors and return the sensitivity of microtubule‐targeting agents after inhibiting the expression of KIF20B. Liu et al. showed that KIF20B knockdown inhibited proliferation of hepatocellular carcinoma cells by stabilizing P53, blocked STAT3 phosphorylation, and prolonged mitotic arrest. In addition, antitumor effect-combined knockdown of KIF20B and taxol was better than taxol alone [14, 15, 25].Totally, this was the first research to demonstrate that KIF20B was upregulated in pancreatic cancer and connected with poor prognosis. KIF20B knockdown inhibited cell proliferation in vitro and in vivo. KIF20B may be a new potential therapeutic target in pancreatic cancer.



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*Source: 5572402-2021-09-09.xml*