# MFG-E8 Exerts Neuroprotection in Neural Stem Cells Induced by Anesthetic Sevoflurane via Regulating the PI3K/AKT Pathways

Authors: Minmin Cai; Liufang Sheng
Journal: Stem Cells International
 (2022)

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## Abstract

MFG-E8 has shown tissue protection effects in various models of organ injury. In this study, the function of MFG-E8 in SEV-induced neural stem cells (NSCs) was studied. The cell viability and apoptosis affected by rhMFG-E8 were tested by MTT and flow cytometry analysis, respectively. Then, the mRNA expression of MFG-E8 was detected by qRT-PCR. The expression of SOD, GSF-Px, and MDA was assessed using ELISA assay. Western blot analysis was applied for assessing the expression of MFG-E8, BCL2, BAX, cleaved caspase-3, GRP-78, XBP-1, ATF-6, ATF-4, CHOP, p-PI3K, PI3K, p-AKT, and AKT. The pharmacological experiments suggested that both mRNA and protein expression of MFG-E8 were significantly decreased after 24 h, 48 h, and 72 h treatment with SEV, and the Western blot results displayed that 50 and 100μg/ml rhMFG-E8 could evidently promote the expression of MFG-E8 in NSCs induced by SEV. Next, rhMFG-E8 reduced the apoptosis of NSCs induced by SEV through upregulating Bcl-2 and cleaved caspase-3 and downregulating Bax. Moreover, rhMFG-E8 alleviated the endoplasmic reticulum pressure of NSCs induced by SEV through decreasing the expression of GRP-78, XBP-1, ATF-6, ATF-4, and CHOP. In addition, the rhMFG-E8 could promote the expression of SOD and GSH-Px and inhibit the expression of MDA and LDH detected by the ELISA assay and LDH kit. Moreover, rhMFG-E8 elevated the expression of p-PI3K/PI3K and p-AKT/AKT, which were inhibited by SEV in NSCs. The results of this project supported that rhMFG-E8 protects neural activity in neural stem cells induced by anesthetic sevoflurane via regulating the PI3K/AKT pathways.


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## Body

## 1. Introduction

Numerous evidence showed that anesthetic processing in early life stages could lead to neurodevelopmental disorders in animal models [1]. The study found that commonly used intravenous and inhalation anesthetics could produce neurotoxicity to cause neuronal death in vitro, even including γ-aminobutyric acid agonists and N-methyl-D-aspartate antagonists [2]. This impairment might affect the development of neural networks, with effects that may persist until adult age, affecting their behavior and recognition abilities.The ability to precisely control in neural stem cells (NSCs) for self-renewal and differentiation [3] plays an essential role in the development and function of the brain [4]. The self-renewal and differentiation of mouse NSCs was inhibited by anesthetics through partially mediating the CaMkII/pS485/AMPK/ATF5 signaling pathway [5]. A previous study demonstrated that repeatedly exposed to sevoflurane (SEV) induced NSCs to differentiate at early stage [6]. Reports have shown that SEV-induced self-renewal of NSCs could inhibit the cell cycle progression of NSCs through the wnt/β-catenin pathway [7]. SEV was able to relieve LPS-induced apoptosis and oxidative stress to protected HK-2 cells by inactivating NF-κB signaling [8]. SEV can also promote the apoptosis of neural stem cells and inhibit their proliferation [9] by disrupting their differentiation ability [10].Milk fat globule epidermal growth factor 8 (MFG-E8) was a lipophilic glycoprotein, expressed in a variety of tissues. MFG-E8 had multiple functions and was involved in a number of cellular processes, such as protecting and restoring of intestinal epithelial cells [11], angiogenesis [12], and clearance of apoptotic cells [13]. MFG-E8 has been shown to treat pancreatic fibrosis in mice through suppressing ER stress-induced autophagy [14]. Human recombinant MFG-E8 (rhMFG-E8) protein attenuated atrial fibrosis and remodeling in animal models by inhibiting the activation of the TGF-β1/Smad2/3 pathway [15]. In addition, MFG-E8 attenuated brain injury of subarachnoid hemorrhage in a rat model by promoting endothelial regeneration and attenuating endothelial DNA damage via stimulating of the TIGβ5/PI3K/CXCL12 signaling pathway [16]. MFG-E8 can also reduce nerve injury in early brain injury after SAH through anti-inflammatory and antiapoptotic effects [17]. However, the role and mechanism of MFG-E8 in anesthesia-induced nerve injury remain unclear.In our study, flow cytometry, ELISA, and Western blot were integrated to explore and identify the function of rhMFG-E8, and the results showed that rhMFG-E8 exerted neuroprotection effects in NSCs induced by anesthetic sevoflurane via regulating the PI3K/AKT pathways.


## 2. Methods

### 2.1. Cell Culture

Human neural stem cells (NSCs) were acquired from National Institutes of Health (Bethesda, Maryland), which belonged to H9 (WA09) human embryonic stem cells. The NSCs were cultured in Complete StemPro® NSC SFM (serum free medium), which could make the NSCs undifferentiated. The StemPro® NSC SFM medium was supplemented with KnockOut™ D-MEM/F-12, 2% StemPro® Neural Supplement, 20 ng/ml of EGF, 20 ng/ml of bFGF, and 2 mM of GlutaMAX™-I. The NSCs were seeded into 24-well plates with a density of5×104 cells/well for 4 days and exposed to 4.1% sevoflurane for 2 h of three consecutive days.


### 2.2. Chemical and Reagents

The rhMFG-E8 protein was acquired from TheraSource (Manhasset, NY). KnockOut™ D-MEM/F-12, Phosphate-buffered saline (PBS), StemPro® Neural Supplement, EGF, bFGF, and GlutaMAX™-I were obtained from Gibco (Grand Island, NY, USA). 3-(4, 5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) was obtained from Sigma (St. Louis, MO, USA). Primary antibodies against MFG-E8, BCL2, BAX, cleaved caspase-3, GRP-78, XBP-1, ATF-6, ATF-4, CHOP, p-PI3K, PI3K, p-AKT, AKT,β-actin, and secondary antibodies IgG were all purchased from Cell Signaling Technology (Danvers, MA, USA).


### 2.3. The 3-(4, 5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazoliumbromide (MTT) Assay

The NSCs were exposed to 4.1% sevoflurane for 2 h of three consecutive days; then the cells were incubated for 6 h at 37°C with SFM containing 0.5 mg/ml MTT. Then, the cells were added with DMSO (150μl/well) for 0.5 h. The 450 nm was used to obtain the absorbance by a microplate reader (Molecular Devices, Silicon Valley, USA).


### 2.4. Flow Cytometry

The percentage of apoptotic cells was assessed by staining with fluorescein isothiocyanate- (FITC-) conjugated Annexin V and propidium iodide (PI) following the manufacturer’s protocols. Cells were analyzed on either a three-laser BD Canto-II or a four-laser BD LSRFortessa X-20, using the FACS Diva software (BD Biosciences). Acquired data files were analyzed using FlowJo 10.01 (BD Biosciences).


### 2.5. The Enzyme-Linked Immunosorbent Assay (ELISA)

ELISA kits were selected to detect the expression of SOD, GSH-Px, and MDA, according to the manufacturer’s instructions.


### 2.6. Lactate Dehydrogenase (LDH) Assay Kit

The expression Pf LDH was detected by the lactate dehydrogenase (LDH) assay kit (#ab65393; Abcam). Cells were seeded into 24-well plates with density of5×104 cells/well for 4 days and exposed to 4.1% sevoflurane for 2 h of three consecutive days. The hNSCs were treated with rhMFG-E8 in 50 and 100 μg/ml, and then the level of LDH was detected according to the manufacturer’s protocol, which was an enzyme marker of plasma membrane damage.


### 2.7. RNA Isolation and Reverse Transcription-Quantitative Polymerase Chain Reaction (RT-qPCR)

The RNA protect cell reagent was applied to isolated total RNA. The qRT-PCR was performed by a QuantStudio™ 7 Flex Real-Time PCR System (Life Technologies, Loughborough, UK) and NovoStart®SYBR qPCR SuperMix Plus. The expression of PSMA1 was normalized against that of GAPDH using the 2−ΔΔCt method and presented as mean±SD of replicates. RNA was reversed transcribed to synthesize cDNA according to the manufacturer’s instructions through the PrimeScript™ RT reagent Kit (Takara, Japan) on an iCycler iQ system (Bio- Rad, USA). The primers for PSMA1 and GAPDH primers are shown in Table 1 [18].Table 1
Sequences of primers in the experiment.
NameSequence (5′ to 3′)MFG-E8-forwardCCGCCTCGTCTGTGTATATGGMFG-E8-reverseCTTGCTATCATAGTTGCTGGCTβ-Actin-forwardACCCTAAGGCCAACCGTGAAβ-Actin-reverseAGAGCATAGCCCTCGTAGATGG


### 2.8. Western Blot Analysis

The total protein was extracted by the modified RIPA buffer (P0013B; Beyotime, Shanghai, China), and the concentration was quantitated by the BCA protein assay kit (P0010; Beyotime, Shanghai, China). Then, the total protein was separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Next, the proteins were transferred onto polyvinylidene fluoride (PVDF) membranes, and then the membranes were blocked in 5% skim milk diluted with TBST for 1 h at room temperature and probed with primary antibodies, including MFG-E8 (R&D Systems, AF2805, 1 : 500), BCL2 (Abcam, ab117115, 1 : 500), BAX (Abcam, ab216494, 1 : 500), cleaved caspase-3 (Abcam, ab90437, 1 : 500), GRP-78 (Abcam, ab230508, 1 : 500), XBP-1 (Abcam, ab230508, 1 : 500), ATF-6 (Abcam, ab203119, 1 : 500), ATF-4 (Abcam, ab23760, 1 : 500), CHOP (Abcam, ab23760, 1 : 500), p-PI3K (Abcam, ab125633, 1 : 500), PI3K (Abcam, ab154583, 1 : 500), p-AKT (Abcam, ab18785, 1 : 1000), AKT (Abcam, ab28422, 1 : 500), andβ-actin (Abcam, ab209857, 1 : 1000) at 4°C overnight. Then, the membranes were incubated with a secondary rabbit anti-rabbit antibody (1 : 1000) the next day at room temperature for 1 h. The ImageJ software was employed for quantitation of the immunoreactive bands.


### 2.9. Statistical Analyses

The SAS 9.0 software was applied to analyze data. The log-rank test was used to assess whether the difference is statistically significant. The difference is deemed to be significant, when the two-tailedp value < 0.05.


## 2.1. Cell Culture

Human neural stem cells (NSCs) were acquired from National Institutes of Health (Bethesda, Maryland), which belonged to H9 (WA09) human embryonic stem cells. The NSCs were cultured in Complete StemPro® NSC SFM (serum free medium), which could make the NSCs undifferentiated. The StemPro® NSC SFM medium was supplemented with KnockOut™ D-MEM/F-12, 2% StemPro® Neural Supplement, 20 ng/ml of EGF, 20 ng/ml of bFGF, and 2 mM of GlutaMAX™-I. The NSCs were seeded into 24-well plates with a density of5×104 cells/well for 4 days and exposed to 4.1% sevoflurane for 2 h of three consecutive days.


## 2.2. Chemical and Reagents

The rhMFG-E8 protein was acquired from TheraSource (Manhasset, NY). KnockOut™ D-MEM/F-12, Phosphate-buffered saline (PBS), StemPro® Neural Supplement, EGF, bFGF, and GlutaMAX™-I were obtained from Gibco (Grand Island, NY, USA). 3-(4, 5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) was obtained from Sigma (St. Louis, MO, USA). Primary antibodies against MFG-E8, BCL2, BAX, cleaved caspase-3, GRP-78, XBP-1, ATF-6, ATF-4, CHOP, p-PI3K, PI3K, p-AKT, AKT,β-actin, and secondary antibodies IgG were all purchased from Cell Signaling Technology (Danvers, MA, USA).


## 2.3. The 3-(4, 5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazoliumbromide (MTT) Assay

The NSCs were exposed to 4.1% sevoflurane for 2 h of three consecutive days; then the cells were incubated for 6 h at 37°C with SFM containing 0.5 mg/ml MTT. Then, the cells were added with DMSO (150μl/well) for 0.5 h. The 450 nm was used to obtain the absorbance by a microplate reader (Molecular Devices, Silicon Valley, USA).


## 2.4. Flow Cytometry

The percentage of apoptotic cells was assessed by staining with fluorescein isothiocyanate- (FITC-) conjugated Annexin V and propidium iodide (PI) following the manufacturer’s protocols. Cells were analyzed on either a three-laser BD Canto-II or a four-laser BD LSRFortessa X-20, using the FACS Diva software (BD Biosciences). Acquired data files were analyzed using FlowJo 10.01 (BD Biosciences).


## 2.5. The Enzyme-Linked Immunosorbent Assay (ELISA)

ELISA kits were selected to detect the expression of SOD, GSH-Px, and MDA, according to the manufacturer’s instructions.


## 2.6. Lactate Dehydrogenase (LDH) Assay Kit

The expression Pf LDH was detected by the lactate dehydrogenase (LDH) assay kit (#ab65393; Abcam). Cells were seeded into 24-well plates with density of5×104 cells/well for 4 days and exposed to 4.1% sevoflurane for 2 h of three consecutive days. The hNSCs were treated with rhMFG-E8 in 50 and 100 μg/ml, and then the level of LDH was detected according to the manufacturer’s protocol, which was an enzyme marker of plasma membrane damage.


## 2.7. RNA Isolation and Reverse Transcription-Quantitative Polymerase Chain Reaction (RT-qPCR)

The RNA protect cell reagent was applied to isolated total RNA. The qRT-PCR was performed by a QuantStudio™ 7 Flex Real-Time PCR System (Life Technologies, Loughborough, UK) and NovoStart®SYBR qPCR SuperMix Plus. The expression of PSMA1 was normalized against that of GAPDH using the 2−ΔΔCt method and presented as mean±SD of replicates. RNA was reversed transcribed to synthesize cDNA according to the manufacturer’s instructions through the PrimeScript™ RT reagent Kit (Takara, Japan) on an iCycler iQ system (Bio- Rad, USA). The primers for PSMA1 and GAPDH primers are shown in Table 1 [18].Table 1
Sequences of primers in the experiment.
NameSequence (5′ to 3′)MFG-E8-forwardCCGCCTCGTCTGTGTATATGGMFG-E8-reverseCTTGCTATCATAGTTGCTGGCTβ-Actin-forwardACCCTAAGGCCAACCGTGAAβ-Actin-reverseAGAGCATAGCCCTCGTAGATGG


## 2.8. Western Blot Analysis

The total protein was extracted by the modified RIPA buffer (P0013B; Beyotime, Shanghai, China), and the concentration was quantitated by the BCA protein assay kit (P0010; Beyotime, Shanghai, China). Then, the total protein was separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Next, the proteins were transferred onto polyvinylidene fluoride (PVDF) membranes, and then the membranes were blocked in 5% skim milk diluted with TBST for 1 h at room temperature and probed with primary antibodies, including MFG-E8 (R&D Systems, AF2805, 1 : 500), BCL2 (Abcam, ab117115, 1 : 500), BAX (Abcam, ab216494, 1 : 500), cleaved caspase-3 (Abcam, ab90437, 1 : 500), GRP-78 (Abcam, ab230508, 1 : 500), XBP-1 (Abcam, ab230508, 1 : 500), ATF-6 (Abcam, ab203119, 1 : 500), ATF-4 (Abcam, ab23760, 1 : 500), CHOP (Abcam, ab23760, 1 : 500), p-PI3K (Abcam, ab125633, 1 : 500), PI3K (Abcam, ab154583, 1 : 500), p-AKT (Abcam, ab18785, 1 : 1000), AKT (Abcam, ab28422, 1 : 500), andβ-actin (Abcam, ab209857, 1 : 1000) at 4°C overnight. Then, the membranes were incubated with a secondary rabbit anti-rabbit antibody (1 : 1000) the next day at room temperature for 1 h. The ImageJ software was employed for quantitation of the immunoreactive bands.


## 2.9. Statistical Analyses

The SAS 9.0 software was applied to analyze data. The log-rank test was used to assess whether the difference is statistically significant. The difference is deemed to be significant, when the two-tailedp value < 0.05.


## 3. Results

### 3.1. The Expression of MFG-E8 Was Decreased in Neural Stem Cells Induced by Sevoflurane

The expression of MFG-E8 was found by qRT-PCR in NSCs induced by SEV at 0, 24 h, 48 h, and 72 h. The results indicated that the level of MFG-E8 was continually decreased in NSCs induced by SEV at 24 h, 48 h, and 72 h (Figure1(a)). The protein expression of MFG-E8 was measured by WB in NSCs induced by SEV at 24 h, 48 h, and 72 h. As shown in Figure 1(b), the expression of MFG-E8 was significantly declined in cells in a time-dependent manner. The results indicated that the MFG-E8 expression was decreased in NSCs induced by SEV. Then, the function of MFG-E8 was investigated in NSCs induced by SEV. As shown in Figure 1(c), the expression of MFG-E8 was downregulated by SEV in NSCs, while the addition of rhMFG-E8 in 50 and 100 μg/ml increased the expression of intracellular MFG-E8. Next, the viability of cells treated with SEV and rhMFG-E8 was detected by MTT. The results indicated that the cells viability was evidently inhibited by SEV compared with control group after 24 h, 48 h, and 72 h. In the groups of 50 and 100 μg/ml rhMFG-E8 treatment, the inhibited cell viability by SEV treatment was significantly enhanced (Figure 1(d)).Figure 1
The expression of MFG-E8 affected by SEV and CA on cell viability NSCs were exposed to 4.1% SEV for 2 h per day for 3 consecutive days). (a) The mRNA expression of MFG-E8 was affected by sevoflurane for 0, 24 h, 48 h, and 72 h. (b) The protein expression of MFG-E8 was affected by sevoflurane for 0, 24 h, 48 h, and 72 h. (c) The protein expression of MFG-E8 was affected by sevoflurane, sevoflurane+50μg/ml rhMFG-E8, and sevoflurane+100 μg/ml rhMFG-E8. (d) The OD value at 450 nm was detected by MTT assay after treatment with sevoflurane, sevoflurane+50 μg/ml rhMFG-E8 and sevoflurane+100 μg/ml rhMFG-E8 for 0, 24 h, 48 h, and 72 h. ∗p<0.005, ∗∗p<0.001, ∗∗∗p<0.0001 (n=3).
(a)(b)(c)(d)


### 3.2. MFG-E8 Could Inhibit Apoptosis of Neural Stem Cells Induced Sevoflurane

To explore the effects of rhMFG-E8 on the apoptosis of neural stem cells induced by SEV, the protein expressions of BAX, Bcl-2, and cleaved caspase-3 were detected by WB. The results shown in Figure2(a) revealed that Bcl-2 protein expression was remarkably decreased in the SEV group. The protein expression of Bcl-2 in NSCs induced by SEV was remarkably increased by rhMFG-E8 at a concentration of 50 and 100 μg/ml, and 100 μg/ml rhMFG-E8 had better effect than 50 μg/ml. According to the results in Figure 2(a), the expression level of Bax was significantly promoted by SEV, and the Bax expression was evidently inhibited by rhMFG-E8 at 50 and 100 μg/ml. Similarly, the expression of cleaved caspase-3 was promoted by SEV, which was inhibited by rhMFG-E8 (50 and 100 μg/ml) in neural stem cells induced by SEV. As shown in Figure 2(b), the apoptosis rate was evidently increased by the SEV group, while rhMFG-E8 at 50 and 100 μg/ml significantly inhibited the cell apoptosis.Figure 2
The apoptosis affected by rhMFG-E8 on NSCs induced by SEV. (a) The protein (BCL2, BAX, and cleaved caspase-3) expression was affected by sevoflurane, sevoflurane+50μg/ml rhMFG-E8 and sevoflurane+100 μg/ml rhMFG-E8. (b) The apoptosis rate was influence by sevoflurane, sevoflurane+50 μg/ml rhMFG-E8, and sevoflurane+100 μg/ml rhMFG-E8. ∗p<0.005, ∗∗p<0.001, ∗∗∗p<0.0001.
(a)(b)


### 3.3. MFG-E8 Could Inhibit Endoplasmic Reticulum Pressure in Neural Stem Cells Induced by Sevoflurane

The above results demonstrated that rhMFG-E8 could promote the viability and inhibit the apoptosis of NSCs induced by SEV. Next, the underlying mechanism of rhMFG-E8 was further explored by detecting the expressions of GRP-78, XBP-1, ATF-6, ATF-4, and CHOP by WB. The results in Figure3 disclosed that all the proteins including GRP-78, XBP-1, ATF-6, ATF-4, and CHOP were promoted by SEV in neural stem cells. The 50 and 100 μg/ml concentration of rhMFG-E8 could alleviate the effect of SEV by declining the protein expression of GRP-78, XBP-1, ATF-6, ATF-4, and CHOP.Figure 3
The expression of protein related with endoplasmic reticulum pressure (GRP-78, XBP-1, ATF-6, ATF-4, and CHOP) affected by sevoflurane, sevoflurane+50μg/ml rhMFG-E8, and sevoflurane+100 μg/ml rhMFG-E8. ∗p<0.005, ∗∗p<0.001, ∗∗∗p<0.0001.


### 3.4. MFG-E8 Could Inhibit Oxidative Stress in Neural Stem Cells Induced by Sevoflurane

The levels of SOD, GSH-Px, and MDA in neural stem cells were measured by ELISA in this study. The result in Figures4(a) and 4(b) revealed that the expressions of SOD and GSH-Px were significantly inhibited by SEV, which were alleviated by 100 μg/ml rhMFG-E8. Moreover, the levels of MDA and LDH were significantly increased by SEV, and 50 and 100 μg/ml of rhMFG-E8 were able to suppress the levels of MDA and LDH in neural stem cells induced by SEV (Figures 4(c) and 4(d)).Figure 4
The study of the mechanism of rhMFG-E8 influencing the protein expression related with oxidative stress in NSCs after culture induced by sevoflurane, sevoflurane+50μg/ml rhMFG-E8, and sevoflurane+100 μg/ml rhMFG-E8. (a) The expression of SOD was detected by ELISA. (b) The expression level of GSH-Px was tested by ELISA. (c) The expression of MDA was detected by ELISA. (d) The LDH release measured by kit. ∗p<0.005, ∗∗p<0.001, ∗∗∗p<0.0001.
(a)(b)(c)(d)


### 3.5. MFG-E8 Could Promote Activation the PI3K/AKT Pathway

To test the action of MFG-E8 on PI3K/AKT pathway, the expressions of p-PI3K, PI3K, p-AKT, and AKT was examined by WB experiment. In Figure5, the results revealed that expression level of p-PI3K/PI3K was downregulated by SEV, and 50 and 100 μg/ml rhMFG-E8 could significantly enhance the expression of p-PI3K/PI3K in neural stem cells induced by SEV. The level of p-AKT/AKT was inhibited by SEV, and the p-AKT/AKT expression was upregulated by 50 and 100 μg/ml rhMFG-E8.Figure 5
The study of the mechanism of CA on PI3K/AKT in the cellular induced by sevoflurane. The protein expression of p-PI3K, PI3K, p-AKT, and AKT was detected by WB.∗p<0.005, ∗∗p<0.001, ∗∗∗p<0.0001.


## 3.1. The Expression of MFG-E8 Was Decreased in Neural Stem Cells Induced by Sevoflurane

The expression of MFG-E8 was found by qRT-PCR in NSCs induced by SEV at 0, 24 h, 48 h, and 72 h. The results indicated that the level of MFG-E8 was continually decreased in NSCs induced by SEV at 24 h, 48 h, and 72 h (Figure1(a)). The protein expression of MFG-E8 was measured by WB in NSCs induced by SEV at 24 h, 48 h, and 72 h. As shown in Figure 1(b), the expression of MFG-E8 was significantly declined in cells in a time-dependent manner. The results indicated that the MFG-E8 expression was decreased in NSCs induced by SEV. Then, the function of MFG-E8 was investigated in NSCs induced by SEV. As shown in Figure 1(c), the expression of MFG-E8 was downregulated by SEV in NSCs, while the addition of rhMFG-E8 in 50 and 100 μg/ml increased the expression of intracellular MFG-E8. Next, the viability of cells treated with SEV and rhMFG-E8 was detected by MTT. The results indicated that the cells viability was evidently inhibited by SEV compared with control group after 24 h, 48 h, and 72 h. In the groups of 50 and 100 μg/ml rhMFG-E8 treatment, the inhibited cell viability by SEV treatment was significantly enhanced (Figure 1(d)).Figure 1
The expression of MFG-E8 affected by SEV and CA on cell viability NSCs were exposed to 4.1% SEV for 2 h per day for 3 consecutive days). (a) The mRNA expression of MFG-E8 was affected by sevoflurane for 0, 24 h, 48 h, and 72 h. (b) The protein expression of MFG-E8 was affected by sevoflurane for 0, 24 h, 48 h, and 72 h. (c) The protein expression of MFG-E8 was affected by sevoflurane, sevoflurane+50μg/ml rhMFG-E8, and sevoflurane+100 μg/ml rhMFG-E8. (d) The OD value at 450 nm was detected by MTT assay after treatment with sevoflurane, sevoflurane+50 μg/ml rhMFG-E8 and sevoflurane+100 μg/ml rhMFG-E8 for 0, 24 h, 48 h, and 72 h. ∗p<0.005, ∗∗p<0.001, ∗∗∗p<0.0001 (n=3).
(a)(b)(c)(d)


## 3.2. MFG-E8 Could Inhibit Apoptosis of Neural Stem Cells Induced Sevoflurane

To explore the effects of rhMFG-E8 on the apoptosis of neural stem cells induced by SEV, the protein expressions of BAX, Bcl-2, and cleaved caspase-3 were detected by WB. The results shown in Figure2(a) revealed that Bcl-2 protein expression was remarkably decreased in the SEV group. The protein expression of Bcl-2 in NSCs induced by SEV was remarkably increased by rhMFG-E8 at a concentration of 50 and 100 μg/ml, and 100 μg/ml rhMFG-E8 had better effect than 50 μg/ml. According to the results in Figure 2(a), the expression level of Bax was significantly promoted by SEV, and the Bax expression was evidently inhibited by rhMFG-E8 at 50 and 100 μg/ml. Similarly, the expression of cleaved caspase-3 was promoted by SEV, which was inhibited by rhMFG-E8 (50 and 100 μg/ml) in neural stem cells induced by SEV. As shown in Figure 2(b), the apoptosis rate was evidently increased by the SEV group, while rhMFG-E8 at 50 and 100 μg/ml significantly inhibited the cell apoptosis.Figure 2
The apoptosis affected by rhMFG-E8 on NSCs induced by SEV. (a) The protein (BCL2, BAX, and cleaved caspase-3) expression was affected by sevoflurane, sevoflurane+50μg/ml rhMFG-E8 and sevoflurane+100 μg/ml rhMFG-E8. (b) The apoptosis rate was influence by sevoflurane, sevoflurane+50 μg/ml rhMFG-E8, and sevoflurane+100 μg/ml rhMFG-E8. ∗p<0.005, ∗∗p<0.001, ∗∗∗p<0.0001.
(a)(b)


## 3.3. MFG-E8 Could Inhibit Endoplasmic Reticulum Pressure in Neural Stem Cells Induced by Sevoflurane

The above results demonstrated that rhMFG-E8 could promote the viability and inhibit the apoptosis of NSCs induced by SEV. Next, the underlying mechanism of rhMFG-E8 was further explored by detecting the expressions of GRP-78, XBP-1, ATF-6, ATF-4, and CHOP by WB. The results in Figure3 disclosed that all the proteins including GRP-78, XBP-1, ATF-6, ATF-4, and CHOP were promoted by SEV in neural stem cells. The 50 and 100 μg/ml concentration of rhMFG-E8 could alleviate the effect of SEV by declining the protein expression of GRP-78, XBP-1, ATF-6, ATF-4, and CHOP.Figure 3
The expression of protein related with endoplasmic reticulum pressure (GRP-78, XBP-1, ATF-6, ATF-4, and CHOP) affected by sevoflurane, sevoflurane+50μg/ml rhMFG-E8, and sevoflurane+100 μg/ml rhMFG-E8. ∗p<0.005, ∗∗p<0.001, ∗∗∗p<0.0001.


## 3.4. MFG-E8 Could Inhibit Oxidative Stress in Neural Stem Cells Induced by Sevoflurane

The levels of SOD, GSH-Px, and MDA in neural stem cells were measured by ELISA in this study. The result in Figures4(a) and 4(b) revealed that the expressions of SOD and GSH-Px were significantly inhibited by SEV, which were alleviated by 100 μg/ml rhMFG-E8. Moreover, the levels of MDA and LDH were significantly increased by SEV, and 50 and 100 μg/ml of rhMFG-E8 were able to suppress the levels of MDA and LDH in neural stem cells induced by SEV (Figures 4(c) and 4(d)).Figure 4
The study of the mechanism of rhMFG-E8 influencing the protein expression related with oxidative stress in NSCs after culture induced by sevoflurane, sevoflurane+50μg/ml rhMFG-E8, and sevoflurane+100 μg/ml rhMFG-E8. (a) The expression of SOD was detected by ELISA. (b) The expression level of GSH-Px was tested by ELISA. (c) The expression of MDA was detected by ELISA. (d) The LDH release measured by kit. ∗p<0.005, ∗∗p<0.001, ∗∗∗p<0.0001.
(a)(b)(c)(d)


## 3.5. MFG-E8 Could Promote Activation the PI3K/AKT Pathway

To test the action of MFG-E8 on PI3K/AKT pathway, the expressions of p-PI3K, PI3K, p-AKT, and AKT was examined by WB experiment. In Figure5, the results revealed that expression level of p-PI3K/PI3K was downregulated by SEV, and 50 and 100 μg/ml rhMFG-E8 could significantly enhance the expression of p-PI3K/PI3K in neural stem cells induced by SEV. The level of p-AKT/AKT was inhibited by SEV, and the p-AKT/AKT expression was upregulated by 50 and 100 μg/ml rhMFG-E8.Figure 5
The study of the mechanism of CA on PI3K/AKT in the cellular induced by sevoflurane. The protein expression of p-PI3K, PI3K, p-AKT, and AKT was detected by WB.∗p<0.005, ∗∗p<0.001, ∗∗∗p<0.0001.


## 4. Discussion and Conclusion

In this study, the SEV-induced neural stem cells (NSCs) were used for investigating the mechanism of rhMFG-E8 on neuroprotection by the clarification of the specific and characteristic protein and signaling pathway. In this study, we demonstrated that the target effects and the specific process of rhMFG-E8 based on characteristic pathways.SEV was able to inhibit the cell cycle progression and proliferation and promote the apoptosis, thereby disrupting the differentiation ability of NSCs [19]. One study suggested that MFG-E8 could reverse microglial-induced neurotoxic astrocyte (A1) via NF-κB and PI3K-Akt pathways [18]. In our study, qRT-PCR and WB results indicated that the expression of MFG-E8 was decreased by SEV and rhMFG-E8 could increase the expression of MFG-E8 in NSCs induced by SEV. Cell viability assays demonstrated that rhMFG-E8 could elevate the viability of NSCs inhibited by SEV. Above results demonstrated that MFG-E8 played essential role in modulating cell viability by regulating targets MFG-E8.The proapoptotic protein Bax and antiapoptotic protein Bcl-2 belong to Bcl-2 family of proteins [20], which are verified to play essential roles in modulating cell apoptosis [21]. The caspase-3 plays a leading role in apoptosis [22]. In our study, the results revealed that rhMFG-E8 could downregulate Bcl-2 and upregulate Bax and cleaved caspase-3 to increase the cell apoptosis that was inhibited by SEV. These results implied that MFG-E8 comprehensively enhanced the cell apoptosis by regulating Bcl-2, Bax, and cleaved caspase-3.Increasing endoplasmic reticulum stress (ERS) might lead to induction of apoptosis [23]. Moreover, ERS-related proteins, glucose-regulated protein 78 (GRP78), C/EBP homologous protein (CHOP), X-box-binding protein-1 (XBP-1), activating transcription factor 4 (ATF 4), and ATF 6 will be activated upon ER stress [24]. The results in our study disclosed that rhMFG-E8 was able to decrease the expression of GRP78, CHOP, XBP-1, and ATF 4, which were increased by SEV in NSCs. Above results suggested that MFG-E8 could inhibit endoplasmic reticulum stress in NSCs induced by SEV.In order to explore the effect of MFG-E8 on oxidative stress, the expressions of SOD, GSH-Px, and MDA were detected. The activities of SOD and GSH-Px were enhanced by rhMFG-E8 in NSCs induced by SEV. The rhMFG-E8 was able to decrease the expression of MDA, which was increased by SEV. The expression of LDH was significantly inhibited by rhMFG-E8 in NSCs induced by SEV. These results indicated that rhMFG-E8 was able to alleviate oxidative stress induced by SEV.The major targets, including AKT and PI3K, in PI3K/AKT pathway played a key role in regulating cell survival [25]. In our study, the expressions of p-PI3K/PI3K and p-AKT/AKT were evidently increased by rhMFG-E8 in NSCs induced by SEV, suggesting that rhMFG-E8 could promote the activation of the PI3K/AKT pathway.In summary, MFG-E8 exerts neuroprotection effects on neural stem cells induced by anesthetic sevoflurane via regulating the PI3K/AKT pathways. In following research, the investigation would be focused on elucidating pharmacodynamic and pharmacological studies of MFG-E8 in vivo and exploring the extra direction of therapeutic targets for neuroprotection.



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*Source: 5609501-2022-10-12.xml*