# Effect of Aging on the Adsorption of Tetracycline by Humic Acid: Insight into the Morphology and Chemical Composition

Authors: Chunli Zheng; Fei He; Zhiwu Cao; Xingxing Cheng; Zhenxing Wang
Journal: Adsorption Science & Technology
 (2022)

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## Abstract

Tetracycline (TC) is a commonly used antibiotic and enters the soil environment continuously. As humic acid (HA) is one of the most important components in soils, it is necessary to investigate the adsorption behavior of TC by HA under different conditions. In this paper, the factor of “aging” was considered. The methodology of coating CaCO3 precipitation on the surface of HA was adopted to simulate the aging process. The adsorption kinetics and isotherm demonstrated that the uptake of TC over HA was quicker with more amount than aged HA, meaning that aging affected the mass transfer of TC from the bulk solution to the outer surface of HA as well as from HA outer surface to its interior pores. To explain the effect of aging, BET was utilized to characterize the morphology of HA and aged HA. It was found that aging resulted in a decrease in specific surface area and pore size. XPS, FTIR, and 2D-COS revealed how aging influenced the chemical composition of HA. Five kinds of functional groups carried by HA contributed to the adsorption of TC, in which the binding affinity towards TC followed the order of −COO>N−H>−C−C>C−O>O−H. These results indicated that -COO was the most sensitive adsorption site. Compared to HA, the content of -COO for aged HA decreased obviously. In summary, aging affected the morphology and chemical position of HA and consequently lead to the change in adsorption kinetics and isotherm of TC.


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## Body

## 1. Introduction

Antibiotics are widely used in human medicine and animal husbandry as they can inhibit the growth and reproduction of bacteria [1]. In 2013, the total antibiotic usage in China was approximately 162000 tons, 150 times high than in the UK, and the total production was estimated to be 248000 tons. It was reported that during the use process 54% of antibiotics would enter into soils [2].The migration and transformation pathways of antibiotics in soils mainly include adsorption by soil particles, absorption by plants, and transportation by runoff. Its well-known adsorption is the key behavior that influences the ultimate fate of antibiotics in soils [3]. The physical and chemical properties of soils such as organic matter, minerals, pH, and moisture affect the adsorption behavior. Among these factors, it is generally believed that organic matter is essential for the adsorption of organic pollutants by soils [4]. Humic acid (HA) is one of the most important components of organic matter. As it contains various functional groups such as hydroxyl, carboxyl, and carbonyl; HA can bind with antibiotics [5]. Vaz et al. [6] extracted HA from a kind of Brazilian soil according to the procedure suggested by the International Humic Substances Society (IHSS) and described the sorption mechanism of oxytetracycline (OTC) on HA. It was found that OTC interacted with the carboxyl group carried by HA, which enhanced the retention degree of OTC in the soil, therefore, the leaching of OTC to the groundwater was impeded. Gu et al. [7] also observed the complex interaction between tetracycline and the carboxylic group of HA.In recent years, more and more studies reported the phenomenon of “aging” that happened in HA in nature [1, 8, 9]. Here, aging means the physical and chemical properties of HA changed with time [10–12]. For example, See and Bronk [13] found an increase in O-H and N-H functional groups carried by humic acid during one year period. Yu et al. [9] pointed out that since the properties of HA change, the adsorption behavior of pollutants correspondingly was affected. Therefore, it is necessary to investigate the influence of “aging” on the adsorption of antibiotics by HA. Till now, the studies that related to preparing “aging” samples mainly consider the factor of time scale. Reeves et al. [14] prepared the aged soil by storing the fresh soil in a stable system for a time scale of 270 d, and then the soil-bound organic pollutants availability was evaluated by in vivo and in vitro measurements. Guthrie et al. [15] prepared the aged sediments by storing the fresh sediments in dark in a 0.05% NaN3 solution for a time scale of 120 d, in which the effect of aging on pyrene transformation and pyrene association with fractions of the sedimentary organic matrix was revealed. Besides the factor of time scale, aging also results from the superficial geochemical process such as precipitation. In the laboratory, scientists usually use the method of coating calcium salt precipitation on the surface of HA or soils to simulate the aging process [16, 17]. The adsorption performance of aged samples is generally investigated from a macroviewpoint, that is, only obtaining the adsorption kinetic and isotherm parameters. However, the exploration of mechanism from a microviewpoint was necessary such as the molecular interaction between the aged samples and target pollutants.In this paper, CaCO3 was used to wrap HA which was extracted from the soil sample based on the method provided by the International Humic Substances Society (IHSS). Tetracycline (TC) was selected as the pollutant as it is one of the most commonly used antibiotics [18]. This paper was helpful to understand the difference in the structure and chemical composition resulting from aging as well as the difference in the TC adsorption behavior brought about by aging.


## 2. Materials and Methods

### 2.1. Soil Sample and Chemicals

The soil samples were collected from the surface of 1-10 cm in an arable field located in the Qinling Mountains (109°15′ 36″ E, 33 °55′ 12″ N), Shaanxi Province, China. The fresh soil samples were dried naturally, sieved through a mesh ( <2 mm), and preserved in a bottle under -4°C. The physiochemical properties of the soil samples were measured in our previous work [19]. Tetracycline hydrochloride (TC, purity>98%) and NaOH (purity>99%) were purchased from Dr. Ehrenstorfer (Germany). CaCl2 (purity>96%) and NaCO3 (purity>98%)were bought from Tianjin TianLi Chemical Reagent Ltd. (China). HCl solution (w/v, 38%) and HF solution (w/v, 40%) were of analytical grade and provided by J&K Scientific Ltd. (Shanghai, China). Ultrapure water was prepared by an ultrapure water instrument (ULUPURE, Sichuan Province, China). N2 (purity>99%) was bought from Xian TaiDa Cryogenic Equipment Ltd. (China).


### 2.2. Preparation of HA and Aged HA

The extraction of humic acid (HA) was operated according to the method provided by the International Humic Substances Society [6]. One hundred grams of the soil samples and 250 mL ultrapure water were mixed in a 500 mL conical flask, and then 1 mol/L of HCl was added until the pH of the mixture reached 1.5. 150 milliliters of HCl with 0.1 mol/L was poured into the 500 mL conical flask. The conical flask was placed in a shaker (Zhicheng, Shanghai, China) at 160 rpm and 25°C for 2 h, and then centrifuged (Xiangyi, Hunan, China) at 3000 rpm for 15 min to remove the supernatant. The pH value of the residual sediment was adjusted to be 7.0 with 1 mol/L of NaOH. Under the protection of nitrogen, 400 ml of NaOH with 0.1 mol/L was added and shaken for 24 hours at 25°C. The flask was centrifuged at 8000 rpm and 25°C for 15 min to collect the supernatant. The pH value of the supernatant was adjusted to be 1.0 with 6 mol/L HCl. Finally, the acidified supernatant was allowed to stand for 24 hours and then centrifuged at 3000 rpm and 25°C for 15 min. After discarding the supernatant, the sediment was freeze-dried at -60°C for 24 hours and named HA (Boyikang, Beijing, China).HA aging was operated based on the method described by An et al. [16] 0.5 g of HA was placed into a 100 mL beaker and mixed with 20 mL of CaCl2 solution with different initial concentrations (0.05 and 0.1 mol/L), respectively. All the beakers were shaken in dark for 24 hours at 25°C and 120 rpm. Twenty milliliters of NaCO3 with 0.05 and 0.1 mol/L were correspondingly added to the beakers and shaken for 48 h to generate CaCO3 precipitation on the surface of HA. The beakers were centrifuged at 8000 rpm and 25°C for 15 min to remove the supernatant. For cleaning the excess calcium carbonate, twenty milliliters of ultrapure water were used, and the cleaning process was repeated five times. Subsequently, the aging samples were obtained and freeze-dried. The two samples were named AHA-0.05 and AHA-0.1, respectively. HA, AHA-0.05, and AHA-0.1 were analyzed by BET, FT-IR, and XPS.


### 2.3. Adsorption Experiments

1.2 g of CaCl2 was mixed with ultrapure water in a 1000 ml volumetric flask to obtain 0.01 mol/L of CaCl2 solution. 0.5 g of TC was dissolved in CaCl2 solution (0.01 mol/L) and then placed in a 500 ml volumetric flask to obtain TC stock solution with 1000 mg/L. The testing TC solutions with different concentrations were prepared by diluting TC stock solution with 0.01 mol/L of CaCl2.Adsorption kinetics: 0.2 g of HA, AHA-0.05, and AHA-0.1 was placed into a 50 mL plastic centrifugal tube, respectively, in which 30 mL of TC testing solutions with 20 mg/L was added. All the tubes were sealed by parafilm and shaken under 120 rpm and 25°C. Samples were withdrawn by a 5 mL pipette periodically and then centrifuged at 8000 rpm and 25°C for 5 min to collect the supernatant. The supernatant was filtered through a 0.45μm membrane (GreenUnion, Jiangsu, China) and analyzed by a UV-Visible spectrophotometer (SP-1915, Spectrum, Shanghai, China) at the wavelength of 355 nm for determining the residual concentration of TC [20]. The calibration curves for TC are as follows: y=0.0324x+0.0307R2=0.997, in which y stands for the absorbing intensity of spectroscopy and x for the concentration of TC. For calculating the adsorption amount qt (mg/g), the equation is as follows:
(1)qt=VC0−C/M,where C0 is the initial concentration of TC (mg/L) in the solution, and C is the residual concentration (mg/L) at time t. V and M are the volumes of TC solution (L) and the mass of sorbent (g), respectively.Adsorption isotherm: 0.2 g of HA, AHA-0.05, and AHA-0.1 was placed into a 50 mL plastic centrifugal tube, respectively, in which 30 mL of TC with different concentrations (5, 10, 20, 30, 40, and 50 mg/L) was added. All the tubes were sealed by parafilm and shaken under 120 rpm and 25°C. Samples were withdrawn by a 5 mL pipette after 24 h and then centrifuged at 8000 rpm and 25°C for 5 min to collect the sediment. The sediment was freeze-dried and then analyzed by FT-IR.All experiments were performed three times and the mean value was used. For the control, each adsorbent was mixed with 0.01 mol/L of CaCl2 solution and operated under identical conditions as mentioned above. The pseudo-first-order kinetic model Equation (2), the pseudo-second-order kinetic model Equation (3), and the Weber and Morris intraparticle diffusion model Equation (4) were used to calculate the kinetic parameters.
(2)Inqe,exp−qt=Inqe,cal−K1t,(3)tqt=1K2qe,cal2+1qe,calt,where qe,exp and qt (mg/g) are the adsorption amount at equilibrium and time t, respectively. K1 is the rate constant of the pseudo-first-order sorption (min-1); K2 is the rate constant of the pseudo-second-order sorption (g/(mg min)).
(4)qt=Kidt1/2+I,where qt (mg/g) is the adsorption amount (mg/L) at any time t; Kid is the rate constant of the intraparticle diffusion model (mg/g.min1/2). I (mg/g) is the intercept, related to the thickness of the boundary layer.The Boyd’s film-diffusion model shown in Equation (5) was used to determine the real control step related to the adsorption process of TC.
(5)Ft=1−6π2∑n=1∞1n2exp−n2Bt,where Ft=qt/qe is the fraction of TC adsorbed at time t and equilibrium time. Bt is a function of F(t). If F(t) values is higher than 0.85.
(6)Bt=0.4977−ln1−Ft.If F(t) values is lower than 0.85,
(7)Bt=π−π−π2Ft32.Two isotherm models, Freundlich and Dubinin-Radushkevich were employed.
(8)qe=KFCe1/n,lnqe=lnqD−R∙T2E2∙ln1+1Ce2,where Ce is the residual concentration of sorbate at equilibrium (mg/L); qe is the uptake of sorbate on per unit mass of sorbent (mg/g); KF (mg/L(1-1/n)·g) and n are the Freundlich constants, which represent the adsorption capacity and intensity, respectively. qD (mg/g) is the Dubinin–Radushkevich monolayer adsorption capacity. R is the gas constant (8.314 kJ/mol), and E is the mean free energy (kJ/mol).


### 2.4. Analysis

The porous structure of HA, AHA-0.05, and AHA-0.1 was measured by a physisorption analyzer (ASAP-2020, Micromeritics, Beijing) at 77 K. The pore size distribution was calculated by the Barrett-Joyner-Halenda (BJH) methods [21]. Scanning Electron Microscopy (SEM, TM-1000, Hitachi, Japan) was used to observe the detailed surface microstructure of HA, AHA-0.05, and AHA-0.1. X-ray photoelectron spectroscopy (XPS) of samples was recorded through Thermo Fisher ESCALAB Xi + via equipping an Mg Kα X-ray source (1253.6 eV protons). Before measurement, the HA and AHA were vacuum desiccated at -60°C for 24 h. The calibration values of O, N, and S were calibrated according to the calibration value of carbon at 284.6 eV. The functional groups of HA and AHA were characterized by Fourier Transform Infrared Spectroscopy (FT-IR) (Nicolet NEXUS 750, USA) with the KBr pellet method and the scanning spectra ranged from 500 to 4000 cm-1. The resolution was 4 cm-1. The FT-IR spectra were analyzed by two-dimensional correlation spectroscopy (2D-COS) as described by [22].


## 2.1. Soil Sample and Chemicals

The soil samples were collected from the surface of 1-10 cm in an arable field located in the Qinling Mountains (109°15′ 36″ E, 33 °55′ 12″ N), Shaanxi Province, China. The fresh soil samples were dried naturally, sieved through a mesh ( <2 mm), and preserved in a bottle under -4°C. The physiochemical properties of the soil samples were measured in our previous work [19]. Tetracycline hydrochloride (TC, purity>98%) and NaOH (purity>99%) were purchased from Dr. Ehrenstorfer (Germany). CaCl2 (purity>96%) and NaCO3 (purity>98%)were bought from Tianjin TianLi Chemical Reagent Ltd. (China). HCl solution (w/v, 38%) and HF solution (w/v, 40%) were of analytical grade and provided by J&K Scientific Ltd. (Shanghai, China). Ultrapure water was prepared by an ultrapure water instrument (ULUPURE, Sichuan Province, China). N2 (purity>99%) was bought from Xian TaiDa Cryogenic Equipment Ltd. (China).


## 2.2. Preparation of HA and Aged HA

The extraction of humic acid (HA) was operated according to the method provided by the International Humic Substances Society [6]. One hundred grams of the soil samples and 250 mL ultrapure water were mixed in a 500 mL conical flask, and then 1 mol/L of HCl was added until the pH of the mixture reached 1.5. 150 milliliters of HCl with 0.1 mol/L was poured into the 500 mL conical flask. The conical flask was placed in a shaker (Zhicheng, Shanghai, China) at 160 rpm and 25°C for 2 h, and then centrifuged (Xiangyi, Hunan, China) at 3000 rpm for 15 min to remove the supernatant. The pH value of the residual sediment was adjusted to be 7.0 with 1 mol/L of NaOH. Under the protection of nitrogen, 400 ml of NaOH with 0.1 mol/L was added and shaken for 24 hours at 25°C. The flask was centrifuged at 8000 rpm and 25°C for 15 min to collect the supernatant. The pH value of the supernatant was adjusted to be 1.0 with 6 mol/L HCl. Finally, the acidified supernatant was allowed to stand for 24 hours and then centrifuged at 3000 rpm and 25°C for 15 min. After discarding the supernatant, the sediment was freeze-dried at -60°C for 24 hours and named HA (Boyikang, Beijing, China).HA aging was operated based on the method described by An et al. [16] 0.5 g of HA was placed into a 100 mL beaker and mixed with 20 mL of CaCl2 solution with different initial concentrations (0.05 and 0.1 mol/L), respectively. All the beakers were shaken in dark for 24 hours at 25°C and 120 rpm. Twenty milliliters of NaCO3 with 0.05 and 0.1 mol/L were correspondingly added to the beakers and shaken for 48 h to generate CaCO3 precipitation on the surface of HA. The beakers were centrifuged at 8000 rpm and 25°C for 15 min to remove the supernatant. For cleaning the excess calcium carbonate, twenty milliliters of ultrapure water were used, and the cleaning process was repeated five times. Subsequently, the aging samples were obtained and freeze-dried. The two samples were named AHA-0.05 and AHA-0.1, respectively. HA, AHA-0.05, and AHA-0.1 were analyzed by BET, FT-IR, and XPS.


## 2.3. Adsorption Experiments

1.2 g of CaCl2 was mixed with ultrapure water in a 1000 ml volumetric flask to obtain 0.01 mol/L of CaCl2 solution. 0.5 g of TC was dissolved in CaCl2 solution (0.01 mol/L) and then placed in a 500 ml volumetric flask to obtain TC stock solution with 1000 mg/L. The testing TC solutions with different concentrations were prepared by diluting TC stock solution with 0.01 mol/L of CaCl2.Adsorption kinetics: 0.2 g of HA, AHA-0.05, and AHA-0.1 was placed into a 50 mL plastic centrifugal tube, respectively, in which 30 mL of TC testing solutions with 20 mg/L was added. All the tubes were sealed by parafilm and shaken under 120 rpm and 25°C. Samples were withdrawn by a 5 mL pipette periodically and then centrifuged at 8000 rpm and 25°C for 5 min to collect the supernatant. The supernatant was filtered through a 0.45μm membrane (GreenUnion, Jiangsu, China) and analyzed by a UV-Visible spectrophotometer (SP-1915, Spectrum, Shanghai, China) at the wavelength of 355 nm for determining the residual concentration of TC [20]. The calibration curves for TC are as follows: y=0.0324x+0.0307R2=0.997, in which y stands for the absorbing intensity of spectroscopy and x for the concentration of TC. For calculating the adsorption amount qt (mg/g), the equation is as follows:
(1)qt=VC0−C/M,where C0 is the initial concentration of TC (mg/L) in the solution, and C is the residual concentration (mg/L) at time t. V and M are the volumes of TC solution (L) and the mass of sorbent (g), respectively.Adsorption isotherm: 0.2 g of HA, AHA-0.05, and AHA-0.1 was placed into a 50 mL plastic centrifugal tube, respectively, in which 30 mL of TC with different concentrations (5, 10, 20, 30, 40, and 50 mg/L) was added. All the tubes were sealed by parafilm and shaken under 120 rpm and 25°C. Samples were withdrawn by a 5 mL pipette after 24 h and then centrifuged at 8000 rpm and 25°C for 5 min to collect the sediment. The sediment was freeze-dried and then analyzed by FT-IR.All experiments were performed three times and the mean value was used. For the control, each adsorbent was mixed with 0.01 mol/L of CaCl2 solution and operated under identical conditions as mentioned above. The pseudo-first-order kinetic model Equation (2), the pseudo-second-order kinetic model Equation (3), and the Weber and Morris intraparticle diffusion model Equation (4) were used to calculate the kinetic parameters.
(2)Inqe,exp−qt=Inqe,cal−K1t,(3)tqt=1K2qe,cal2+1qe,calt,where qe,exp and qt (mg/g) are the adsorption amount at equilibrium and time t, respectively. K1 is the rate constant of the pseudo-first-order sorption (min-1); K2 is the rate constant of the pseudo-second-order sorption (g/(mg min)).
(4)qt=Kidt1/2+I,where qt (mg/g) is the adsorption amount (mg/L) at any time t; Kid is the rate constant of the intraparticle diffusion model (mg/g.min1/2). I (mg/g) is the intercept, related to the thickness of the boundary layer.The Boyd’s film-diffusion model shown in Equation (5) was used to determine the real control step related to the adsorption process of TC.
(5)Ft=1−6π2∑n=1∞1n2exp−n2Bt,where Ft=qt/qe is the fraction of TC adsorbed at time t and equilibrium time. Bt is a function of F(t). If F(t) values is higher than 0.85.
(6)Bt=0.4977−ln1−Ft.If F(t) values is lower than 0.85,
(7)Bt=π−π−π2Ft32.Two isotherm models, Freundlich and Dubinin-Radushkevich were employed.
(8)qe=KFCe1/n,lnqe=lnqD−R∙T2E2∙ln1+1Ce2,where Ce is the residual concentration of sorbate at equilibrium (mg/L); qe is the uptake of sorbate on per unit mass of sorbent (mg/g); KF (mg/L(1-1/n)·g) and n are the Freundlich constants, which represent the adsorption capacity and intensity, respectively. qD (mg/g) is the Dubinin–Radushkevich monolayer adsorption capacity. R is the gas constant (8.314 kJ/mol), and E is the mean free energy (kJ/mol).


## 2.4. Analysis

The porous structure of HA, AHA-0.05, and AHA-0.1 was measured by a physisorption analyzer (ASAP-2020, Micromeritics, Beijing) at 77 K. The pore size distribution was calculated by the Barrett-Joyner-Halenda (BJH) methods [21]. Scanning Electron Microscopy (SEM, TM-1000, Hitachi, Japan) was used to observe the detailed surface microstructure of HA, AHA-0.05, and AHA-0.1. X-ray photoelectron spectroscopy (XPS) of samples was recorded through Thermo Fisher ESCALAB Xi + via equipping an Mg Kα X-ray source (1253.6 eV protons). Before measurement, the HA and AHA were vacuum desiccated at -60°C for 24 h. The calibration values of O, N, and S were calibrated according to the calibration value of carbon at 284.6 eV. The functional groups of HA and AHA were characterized by Fourier Transform Infrared Spectroscopy (FT-IR) (Nicolet NEXUS 750, USA) with the KBr pellet method and the scanning spectra ranged from 500 to 4000 cm-1. The resolution was 4 cm-1. The FT-IR spectra were analyzed by two-dimensional correlation spectroscopy (2D-COS) as described by [22].


## 3. Results and Discussion

### 3.1. Characterization of HA and AHA

As shown in the inserts of Figures1(a) and 1(b), the extracted HA (named as HA) was black in color, and the HA after being treated with 0.1 mol/L of CaCO3 (named as AHA) was charcoal gray. The reason for the color change might be the formation of white CaCO3 deposits. The SEM images (Figures 1(a) and 1(b)) exhibited that HA was of aggregated and irregular structure (in red frame) while AHA was composed of massive particles. On the surface of AHA, a kind of regular block (in red frame) was observed, presumably attributing to calcium carbonate. EDS analysis demonstrated the weight percentage of Ca on the surface of HA was 5.5% while increased to 8.3% for AHA (Figures 1(a) and 1(b)), respectively. A similar phenomenon was found for oxygen elements. Based on SEM and EDS analysis, it can be concluded that CaCO3 precipitation was successfully coated on the surface of HA to generate AHA.Figure 1
(a) SEM image of HA: insert is the outlook of HA; (b) SEM image of AHA: insert is the outlook of AHA.
(a)
(b)


### 3.2. Adsorption of TC over HA and AHA Samples

#### 3.2.1. Adsorption of TC at Different Contact Times

The effect of contact time on the adsorption of TC by HA, AHA-0.05, and AHA-0.1 was evaluated (Figure2(a)), in which AHA-0.05 and AHA-0.1 indicated the concentration of CaCO3 used for wrapping HA was 0.05 mol/L and 0.1 mol/L, respectively. All the adsorption processes were divided into three stages according to the variation trend of qt along with contact time: stage 1 (0-120 min), stage 2 (120-1320 min), and stage 3 (1320-3000 min). The aforementioned three stages corresponded to a rapid increase in qt value, a slight increase in qt value, and an almost unchanged qt value. The equilibrium times were determined at 1320 min, and the equilibrium adsorption capacities (qe) of HA, AHA-0.05, and AHA-0.1 were 2.82, 2.48, and 2.17 mg/g, respectively. The removal efficiency of TC by HA at equilibrium time was more than 90%, obviously higher than it for AHA-0.05 (81%) and AHA-0.1 (71%) (the insert in Figure 2(a)).Figure 2
(a) Effect of contact time on the adsorption of TC in which the initial concentration was 20 mg/L (insert is the removal efficiency of TC under different contact time); (b) ratio ofqt to qe under different contact time; (c) ratio of qt between two different adsorbents; (d) the parameters of different models (model 1: pseudo-second-order model; model 2: Weber and Morris intraparticle diffusion model; model 3: Freundlich model).
(a)
(b)
(c)
(d)As for stage 1 (0-120 min), the ratio of qt at 30 min toqe for AHA-0.1 and AHA-0.05 was 0.82 and 0.84, respectively, while for HA only 0.55 (Figure 2(b)). When the contact time was prolonged to 120 min, the corresponding ratios (qt,120min/qe) were 0.86, 0.87, and 0.98. Interestingly, the value of qt,120min minus qt,30min equaled 0.43 for HA whereas less than 0.04 for the two AHA samples. These results indicated that for AHA most of the adsorption happened in the first 30 min. The possible reason was that the numbers of adsorption sites on the surface of AHA-0.1 and AHA-0.05 were less than those of HA due to the formation of CaCO3 deposits. TC molecular would occupy most of the adsorption sites on the surfaces of AHA-0.1 and AHA-0.05 at the initial 30 min owing to the limited numbers in adsorption sites. Different from AHA samples, HA provided adequate adsorption sites. Therefore, TC may attach to the sites with higher energy and then the sites with lower energy, possibly resulting in a relatively slower increase in the value of qt in the initial 30 min.The values ofqt at different contact times for the three adsorbents were treated by using a pair-wise comparison, and the results are shown in Figure 2(c). The ratios of qt,AHA-0.05/qt,AHA-0.1 were almost maintained at 1.2 during the whole adsorption process from 10 to 2880 min. The ratios of qt,HA/qt,AHA-0.05 and qt,HA/qt,AHA-0.1 increased quickly from 10 to 240 min, in which the values reached the maximum at 240 min (1.3 for the former and 1.5 for the latter). From 240 to 1320 min, the ratios began to decrease and then kept unchanged from 1320 to 2880 min. As qt,HA/qt,AHA-0.05 multiply by qt,AHA-0.05/qt,AHA-0.1 equals to qt,HA/qt,AHA-0.1, as well as qt,AHA-0.05/qt,AHA-0.1 was almost fixed at 1.2, which means that the values of qt,HA/qt,AHA-0.1 were 1.2 times higher than those of qt,HA/qt,AHA-0.05 during the whole adsorption process from 0 to 2880 min. The difference between qt,HA/qt,AHA and qt,AHA-0.05/qt,AHA-0.1 was the biggest at 10 min and then shrank at 30 min, which further proved that most of the adsorption for AHA samples occurred in the initial stage even at the first 10 minutes.


#### 3.2.2. Adsorption Kinetics and Isotherm

The pseudo-first-order kinetic model and the pseudo-second-order kinetic model were used and all the parameters were listed in Table1 and Figure 3(a). The pseudo-second-order kinetic model presented a better fit for HA, AHA-0.05, and AHA-0.1 based on the values of R2. Furthermore, the calculated qe values (2.841, 2.488, and 2.197 mg/g for HA, AHA-0.05, and AHA-0.1, respectively) were close to the experiment data (2.82, 2.48, and 2.17 mg/g, respectively). From Table 1, it can be seen that the value of K2q2e,cal for HA was larger than those for AHA-0.05 and AHA-0.1, indicating that the initial adsorption rate of TC by the three adsorbents followed the order of HA>AHA−0.05>AHA−0.1.Table 1
Parameters of the kinetic models for the adsorption of TC by HA and AHA samples.
Models
Parameters
HA
AHA-0.05
AHA-0.1
Pseudo-first-order
qe,exp/mg·g-1
2.82
2.48
2.17
qe,cal/mg·g-1
1.383
1.274
1.139
K1/h-1
0.0005
0.001
0.001
R
2
0.852
0.827
0.831
Pseudo-second-order
K
2/g·mg-1·h-1
0.037
0.02
0.02
qe,cal/mg·g-1
2.841
2.488
2.197
R
2
0.999
0.999
0.992
K
2
q
2
e,cal/mg·g-1·h-1
0.299
0.125
0.096
Weber and Morris intraparticle diffusion
Ki
d1/mg·g-1·h-1/2
0.276
0.041
0.039
I
1/mg·g-1
-0.115
1.493
1.742
R
2
0.944
0.923
—
Ki
d2/mg·g-1·h-1/2
0.0004
0.0085
0.0860
I
2/mg·g-1
2.8100
2.0850
1.7830
R
2
0.831
0.650
—
Boyd’s film-diffusion model
Intercept
-0.702
0.906
0.845
R
2
0.955
0.744
0.648Figure 3
(a) The pseudo-second-order kinetic model fitting the adsorption of TC onto HA, AHA-0.05 and AHA-0.1, respectively (insert is the e Boyd’s film-diffusion model fitting the adsorption of TC onto HA, AHA-0.05 and AHA-0.1, respectively); (b) the Weber and Morris intraparticle diffusion model fitting the adsorption of TC onto HA, AHA-0.05, and AHA-0.1, respectively.
(a)
(b)The Weber and Morris intraparticle diffusion model was used to investigate the rate-limiting step, and all the fitting dates are shown in Table1. As shown in Figure 3(b), it was found that the fitted curves can be separated into two parts distinctly. All the first lines did not pass through the origin, suggesting that the rate-controlling step for the adsorption of TC on HA, AHA-0.05, and, AHA-0.1 proceeded by the boundary layer and intraparticle diffusion simultaneously. The diffusion coefficients of Kid1 for HA (0.276 mg/g.h1/2) were higher than those for AHA-0.05 (0.041 mg/g.h1/2) and AHA-0.1(0.039 mg/g.h1/2), indicating that the deposits of CaCO3 existing on the surface of AHA reduced the mass transfer rate of TC from the aqueous liquid to the external surface of the adsorbent. Fan et al. [23] indicated that when the curve fitted by the Weber and Morris internal particle diffusion model is extended to y-axis, the intercept obtained is proportional to the thickness of the boundary layer. The larger the intercept is, the thicker the boundary layer has. In this study, the values of I1 for HA, AHA-0.05, and AHA-0.1 were -0.115, 1.493, and 1.742 mg/g, respectively, indicating that when HA was coated with a higher concentration of calcium carbonate, more calcium carbonate precipitates accumulated on the surface of AHA.To further determined the real control step of the adsorption process of TC. The Boyd’s film diffusion model was used. Table1 showed the results of adsorption kinetics. If the plot was nonlinear or linear but does not passes through the origin, the adsorption rate was controlled by film-diffusion. If the plot of Bt against time was linear and passed through the origin, intraparticle diffusion controls the rate of mass transfer [23]. Based on the results from Table 1 and the insert of Figure 3(a), although the plot of Bt against time was linear but the intercept of the plots of HA, AHA-0.05, and AHA-0.1, adsorption are -0.702, 0.906, and 0.845, which were significantly different from zero. The intercept of the plots of AHA was farther away from zero, and according to the result of the introparticle diffusion plot, it is indicated that the adsorption rate of TC on AHA was more greatly affected by film diffusion.Freundlich and D-R model fitting results are shown in Figure4, and the fitting parameters are shown in Table 2. For the Freundlich model, the value of R2 for HA, AHA-0.05, and AHA-0.1 was 0.960, 0.920, and 0.972, respectively, while for the Dubinin-Radushkevich model the values of R2 were correspondingly 0.920, 0.970 and 0.900. Adsorption free energy E can be determined from the D-R isotherm, which gives information about the adsorption nature. If the magnitude of E is between 8 and 16 kJ/mol, the sorption process is chemical. If the value of E is less than 8 kJ/mol, the sorption process is supposed to proceed via physical sorption [24]. Based on Table 2, it can be concluded that the adsorption nature of the TC on HA, AHA-0.05, and AHA-0.1 was physisorption.Figure 4
(a) Freundlich model fitting the adsorption of TC on the AHA-0.05 and AHA-0.1 HA, and insert is the HA; (b) Dubinin-Radushkevich model fitting the adsorption of TC on the AHA-0.05 and AHA-0.1, and insert is the HA.
(a)
(b)Table 2
Parameters of the isotherm models describing the sorption of TC.
Model
Parameters
HA
AHA-0.05
AHA-0.1
D-R
E/kJ·Mol-1
2.083
1.730
1.740
qD/mg·g-1
33.115
13.197
8.248
R
2
0.920
0.970
0.900
Freundlich
K
F/mg·L-(1-1/n)·g-1
0.016
0.003
0.0005
n
0.296
0.340
0.381
R
2
0.960
0.920
0.972


#### 3.2.3. Adsorption Performance Comparison

Figure2(d) compared the kinetic and isotherm parameters among the three adsorbents. The parameter of K2q2e,cal derived from the pseudo-second-order kinetic model represents the initial adsorption rate [19]. It can be seen that HA possessed the fastest initial adsorption rate then followed by AHA-0.05 and AHA-0.1. The diffusion coefficient of Kid1 from the Weber and Morris intraparticle diffusion model followed the order of HA>AHA−0.05>AHA−0.1. The possible reason is that the formation of CaCO3 deposits on the surface of AHA samples impeded the mass transfer of TC from solution bulk to the outer surface of the adsorbents [20]. The values of Kid1 can also explain why the parameter of K2q2e,cal for HA was significantly bigger than those for AHA-0.05 and AHA-0.1. Based on the values of KF (Freundlich constant), it can be concluded that HA adsorbed more amounts of TC than AHA samples. These results demonstrated that once HA was wrapped by CaCO3, the adsorption capacity towards TC decreased obviously.


### 3.3. Adsorption Mechanism of TC by HA and AHA Samples

#### 3.3.1. Morphology Analysis

As shown in Figure5(a), HA, AHA-0.05, and AHA-0.1 samples exhibited type-III adsorption isothermal curves, in which the BET specific surface areas were calculated to be 5, 3, and 2 m2·g-1, respectively. The correspondingly total pore volumes were 0.08, 0.06 and 0.05 cm3·g-1 (P/P0=0.9933) (Figure 5(b)). The decreasing percentages (SHA−SAHA/SHA) of AHA-0.05 to HA and AHA-0.1 to HA in BET specific surface areas were 44% and 50%, respectively. As can be seen from Figure 5(b), the pore sizes of the three adsorbents are mainly concentrated at 30 nm (HA), 28 nm (AHA-0.05), and 20 nm (AHA-0.1), respectively. These results demonstrated that the formation of CaCO3 deposits on the surface of AHA samples rendered them with lower specific surface areas and smaller pore sizes compared to HA, which explains why the HA samples possessed quicker adsorption kinetics and higher adsorption amount.Figure 5
(a) Nitrogen sorption-desorption isotherm for HA, insert for AHA samples; (b) pore size distribution of HA, AHA-0.05 and AHA-0.1.
(a)
(b)


#### 3.3.2. Chemical Composition Analysis

In addition to the pore analysis, the morphology and the functional groups of fresh HA and aged HA were compared. The adsorption mechanisms of TC on fresh HA and aged HA were explored. The functional groups of the tree adsorbents were also analyzed by XPS technology to explain the adsorption mechanism from the viewpoint of molecular interaction. As shown in Figure6(a), the full scan survey XPS spectra revealed that HA, AHA-0.05, and AHA-0.1 were mainly composed of C and O as well as small amounts of Si and Al. Interestingly, a new peak of Ca 2p emerged for the two AHA samples, suggesting that calcium carbonate covered their surfaces. Figures 6(b)–6(d) are the XPS high-resolution graphs of C 1 s for HA, AHA-0.05, and, AHA-0.1, in which it was found that three states of carbon existed. It is well known that the C element has four kinds of binding forms. The first one is carbon atom links with carbon or hydrogen atom to form C-C or C-H corresponding to relatively lower binding energy (285 eV) [25]. The second one is the carbon atom connects with the carbonyl oxygen atom to form C-O at 286. 5 eV. The third one is carbon atom binds with two oxygen atoms (O-C-O) or connects with one atom of oxygen to form C=O, in which the energy spectrum peak locate between 288~288.5 eV [26]. The last one is a carbon atom that reacts with two oxygen atoms to generate O−C=O under high binding energy of 289~289.5 eV [26]. Therefore, it can be deduced that HA possessed the functional groups of C-C or C-H (284.8 eV), C-O (286.3 eV), and O-C=O bond (289.1 eV) (Figure 6(b)). Similar conclusions can be used for AHA-0.05 and AHA-0.1 (Figures 6(c) and 6(d)). Furthermore, the content of the three functional groups was calculated, and the results were given in Figure 6(e). It was observed that after being wrapped by CaCO3, the percentage of O−C=O for the two AHA samples decreased obviously compared to HA and followed the order of HA>AHA−0.05>AHA−0.1.Figure 6
(a) XPS full scan spectra of HA and AHA samples; high resolution C1s XPS spectra of (b) HA (c) AHA-0.05, and (d) AHA-0.1; (e) the percentage of different functional groups carried by HA, AHA-0.05 and AHA-0.1.
(a)
(b)
(c)
(d)
(e)The FT-IR spectra of HA before and after adsorption of TC are shown in Figure7(a). The peak at 3416 cm-1 was attributed to the stretching vibration of phenolic hydroxyl or alcohol hydroxyl [27]. The peak at 1572 cm-1 was identified as N-H bending or C-N stretching vibration of amide II [19]. The peak at 1401 cm-1 belonged to the symmetric vibration of carboxylate [28]. A sharp band at 1032 cm-1 was assigned to C-O, which indicated the occurrence of carbohydrates [29]. The sharp peak at 912 cm-1 belonged to the vibration of the aliphatic skeleton [27]. 2D-COS was further used to analyze the FT-IR to sequence the binding affinity of HA towards TC. On the diagonals of the synchronization graph (Figure 7(b)), there are five self-intersecting positive peaks appeared at the main diagonal, as well as five cross-peaks were observed on both sides of the diagonal, indicating that the signal of functional groups on the surface of HA changed along with the change in TC concentrations. In the asynchronous map (Figure 7(c)), six cross-peaks located at 912/1032 cm-1, 912/3416 cm-1, 1032/3416 cm-1, 1401/1572 cm-1, 1572/3416 cm-1, and 1401/3416 cm-1 were positive. Four negative peaks were located at 912/1401 cm-1, 912/1572 cm-1, 1032/1401 cm-1, and 1032/1572 cm-1. Table 3 lists the signs of each cross peak in the synchronous (Ф) and asynchronous (Ψ) maps. According to Noda’s rule [30], it can be concluded that the sequence of the binding affinities of TC was −COO>N−H>−C−C>C−O>O−H, which means that -COO was the most sensitive group.Figure 7
(a) FT-IR spectra of HA sample and HA after adsorbed different concentrations of TC; (b) synchronous and (c) asynchronous 2D correlation maps generated from the FT-IR.
(a)
(b)
(c)Table 3
Signs of each cross peak in the synchronous (Ф) and asynchronous (Ψ) maps.
Position (cm-1)
Sign
912
1032
1401
1572
3416
912
+
+
—
—
+
1032
+
—
—
+
1401
+
+
+
1572
+
+
3416
+
(signs were obtained in the maps of 2D-COS map, + positive and - negative).The analysis result from XPS (Figure6(e)) proved that the content of -COO decreased once HA was wrapped by CaCO3 deposits, in which it followed the order of HA>AHA−0.05>AHA−0.1. As FTIR-2D-COS indicates that -COO was the functional group with the highest affinity towards TC, it explains why HA possessed the biggest adsorption amounts and fastest adsorption kinetics and then followed by AHA-0.05 and AHA-0.1 (Figure 2(d)).


## 3.1. Characterization of HA and AHA

As shown in the inserts of Figures1(a) and 1(b), the extracted HA (named as HA) was black in color, and the HA after being treated with 0.1 mol/L of CaCO3 (named as AHA) was charcoal gray. The reason for the color change might be the formation of white CaCO3 deposits. The SEM images (Figures 1(a) and 1(b)) exhibited that HA was of aggregated and irregular structure (in red frame) while AHA was composed of massive particles. On the surface of AHA, a kind of regular block (in red frame) was observed, presumably attributing to calcium carbonate. EDS analysis demonstrated the weight percentage of Ca on the surface of HA was 5.5% while increased to 8.3% for AHA (Figures 1(a) and 1(b)), respectively. A similar phenomenon was found for oxygen elements. Based on SEM and EDS analysis, it can be concluded that CaCO3 precipitation was successfully coated on the surface of HA to generate AHA.Figure 1
(a) SEM image of HA: insert is the outlook of HA; (b) SEM image of AHA: insert is the outlook of AHA.
(a)
(b)


## 3.2. Adsorption of TC over HA and AHA Samples

### 3.2.1. Adsorption of TC at Different Contact Times

The effect of contact time on the adsorption of TC by HA, AHA-0.05, and AHA-0.1 was evaluated (Figure2(a)), in which AHA-0.05 and AHA-0.1 indicated the concentration of CaCO3 used for wrapping HA was 0.05 mol/L and 0.1 mol/L, respectively. All the adsorption processes were divided into three stages according to the variation trend of qt along with contact time: stage 1 (0-120 min), stage 2 (120-1320 min), and stage 3 (1320-3000 min). The aforementioned three stages corresponded to a rapid increase in qt value, a slight increase in qt value, and an almost unchanged qt value. The equilibrium times were determined at 1320 min, and the equilibrium adsorption capacities (qe) of HA, AHA-0.05, and AHA-0.1 were 2.82, 2.48, and 2.17 mg/g, respectively. The removal efficiency of TC by HA at equilibrium time was more than 90%, obviously higher than it for AHA-0.05 (81%) and AHA-0.1 (71%) (the insert in Figure 2(a)).Figure 2
(a) Effect of contact time on the adsorption of TC in which the initial concentration was 20 mg/L (insert is the removal efficiency of TC under different contact time); (b) ratio ofqt to qe under different contact time; (c) ratio of qt between two different adsorbents; (d) the parameters of different models (model 1: pseudo-second-order model; model 2: Weber and Morris intraparticle diffusion model; model 3: Freundlich model).
(a)
(b)
(c)
(d)As for stage 1 (0-120 min), the ratio of qt at 30 min toqe for AHA-0.1 and AHA-0.05 was 0.82 and 0.84, respectively, while for HA only 0.55 (Figure 2(b)). When the contact time was prolonged to 120 min, the corresponding ratios (qt,120min/qe) were 0.86, 0.87, and 0.98. Interestingly, the value of qt,120min minus qt,30min equaled 0.43 for HA whereas less than 0.04 for the two AHA samples. These results indicated that for AHA most of the adsorption happened in the first 30 min. The possible reason was that the numbers of adsorption sites on the surface of AHA-0.1 and AHA-0.05 were less than those of HA due to the formation of CaCO3 deposits. TC molecular would occupy most of the adsorption sites on the surfaces of AHA-0.1 and AHA-0.05 at the initial 30 min owing to the limited numbers in adsorption sites. Different from AHA samples, HA provided adequate adsorption sites. Therefore, TC may attach to the sites with higher energy and then the sites with lower energy, possibly resulting in a relatively slower increase in the value of qt in the initial 30 min.The values ofqt at different contact times for the three adsorbents were treated by using a pair-wise comparison, and the results are shown in Figure 2(c). The ratios of qt,AHA-0.05/qt,AHA-0.1 were almost maintained at 1.2 during the whole adsorption process from 10 to 2880 min. The ratios of qt,HA/qt,AHA-0.05 and qt,HA/qt,AHA-0.1 increased quickly from 10 to 240 min, in which the values reached the maximum at 240 min (1.3 for the former and 1.5 for the latter). From 240 to 1320 min, the ratios began to decrease and then kept unchanged from 1320 to 2880 min. As qt,HA/qt,AHA-0.05 multiply by qt,AHA-0.05/qt,AHA-0.1 equals to qt,HA/qt,AHA-0.1, as well as qt,AHA-0.05/qt,AHA-0.1 was almost fixed at 1.2, which means that the values of qt,HA/qt,AHA-0.1 were 1.2 times higher than those of qt,HA/qt,AHA-0.05 during the whole adsorption process from 0 to 2880 min. The difference between qt,HA/qt,AHA and qt,AHA-0.05/qt,AHA-0.1 was the biggest at 10 min and then shrank at 30 min, which further proved that most of the adsorption for AHA samples occurred in the initial stage even at the first 10 minutes.


### 3.2.2. Adsorption Kinetics and Isotherm

The pseudo-first-order kinetic model and the pseudo-second-order kinetic model were used and all the parameters were listed in Table1 and Figure 3(a). The pseudo-second-order kinetic model presented a better fit for HA, AHA-0.05, and AHA-0.1 based on the values of R2. Furthermore, the calculated qe values (2.841, 2.488, and 2.197 mg/g for HA, AHA-0.05, and AHA-0.1, respectively) were close to the experiment data (2.82, 2.48, and 2.17 mg/g, respectively). From Table 1, it can be seen that the value of K2q2e,cal for HA was larger than those for AHA-0.05 and AHA-0.1, indicating that the initial adsorption rate of TC by the three adsorbents followed the order of HA>AHA−0.05>AHA−0.1.Table 1
Parameters of the kinetic models for the adsorption of TC by HA and AHA samples.
Models
Parameters
HA
AHA-0.05
AHA-0.1
Pseudo-first-order
qe,exp/mg·g-1
2.82
2.48
2.17
qe,cal/mg·g-1
1.383
1.274
1.139
K1/h-1
0.0005
0.001
0.001
R
2
0.852
0.827
0.831
Pseudo-second-order
K
2/g·mg-1·h-1
0.037
0.02
0.02
qe,cal/mg·g-1
2.841
2.488
2.197
R
2
0.999
0.999
0.992
K
2
q
2
e,cal/mg·g-1·h-1
0.299
0.125
0.096
Weber and Morris intraparticle diffusion
Ki
d1/mg·g-1·h-1/2
0.276
0.041
0.039
I
1/mg·g-1
-0.115
1.493
1.742
R
2
0.944
0.923
—
Ki
d2/mg·g-1·h-1/2
0.0004
0.0085
0.0860
I
2/mg·g-1
2.8100
2.0850
1.7830
R
2
0.831
0.650
—
Boyd’s film-diffusion model
Intercept
-0.702
0.906
0.845
R
2
0.955
0.744
0.648Figure 3
(a) The pseudo-second-order kinetic model fitting the adsorption of TC onto HA, AHA-0.05 and AHA-0.1, respectively (insert is the e Boyd’s film-diffusion model fitting the adsorption of TC onto HA, AHA-0.05 and AHA-0.1, respectively); (b) the Weber and Morris intraparticle diffusion model fitting the adsorption of TC onto HA, AHA-0.05, and AHA-0.1, respectively.
(a)
(b)The Weber and Morris intraparticle diffusion model was used to investigate the rate-limiting step, and all the fitting dates are shown in Table1. As shown in Figure 3(b), it was found that the fitted curves can be separated into two parts distinctly. All the first lines did not pass through the origin, suggesting that the rate-controlling step for the adsorption of TC on HA, AHA-0.05, and, AHA-0.1 proceeded by the boundary layer and intraparticle diffusion simultaneously. The diffusion coefficients of Kid1 for HA (0.276 mg/g.h1/2) were higher than those for AHA-0.05 (0.041 mg/g.h1/2) and AHA-0.1(0.039 mg/g.h1/2), indicating that the deposits of CaCO3 existing on the surface of AHA reduced the mass transfer rate of TC from the aqueous liquid to the external surface of the adsorbent. Fan et al. [23] indicated that when the curve fitted by the Weber and Morris internal particle diffusion model is extended to y-axis, the intercept obtained is proportional to the thickness of the boundary layer. The larger the intercept is, the thicker the boundary layer has. In this study, the values of I1 for HA, AHA-0.05, and AHA-0.1 were -0.115, 1.493, and 1.742 mg/g, respectively, indicating that when HA was coated with a higher concentration of calcium carbonate, more calcium carbonate precipitates accumulated on the surface of AHA.To further determined the real control step of the adsorption process of TC. The Boyd’s film diffusion model was used. Table1 showed the results of adsorption kinetics. If the plot was nonlinear or linear but does not passes through the origin, the adsorption rate was controlled by film-diffusion. If the plot of Bt against time was linear and passed through the origin, intraparticle diffusion controls the rate of mass transfer [23]. Based on the results from Table 1 and the insert of Figure 3(a), although the plot of Bt against time was linear but the intercept of the plots of HA, AHA-0.05, and AHA-0.1, adsorption are -0.702, 0.906, and 0.845, which were significantly different from zero. The intercept of the plots of AHA was farther away from zero, and according to the result of the introparticle diffusion plot, it is indicated that the adsorption rate of TC on AHA was more greatly affected by film diffusion.Freundlich and D-R model fitting results are shown in Figure4, and the fitting parameters are shown in Table 2. For the Freundlich model, the value of R2 for HA, AHA-0.05, and AHA-0.1 was 0.960, 0.920, and 0.972, respectively, while for the Dubinin-Radushkevich model the values of R2 were correspondingly 0.920, 0.970 and 0.900. Adsorption free energy E can be determined from the D-R isotherm, which gives information about the adsorption nature. If the magnitude of E is between 8 and 16 kJ/mol, the sorption process is chemical. If the value of E is less than 8 kJ/mol, the sorption process is supposed to proceed via physical sorption [24]. Based on Table 2, it can be concluded that the adsorption nature of the TC on HA, AHA-0.05, and AHA-0.1 was physisorption.Figure 4
(a) Freundlich model fitting the adsorption of TC on the AHA-0.05 and AHA-0.1 HA, and insert is the HA; (b) Dubinin-Radushkevich model fitting the adsorption of TC on the AHA-0.05 and AHA-0.1, and insert is the HA.
(a)
(b)Table 2
Parameters of the isotherm models describing the sorption of TC.
Model
Parameters
HA
AHA-0.05
AHA-0.1
D-R
E/kJ·Mol-1
2.083
1.730
1.740
qD/mg·g-1
33.115
13.197
8.248
R
2
0.920
0.970
0.900
Freundlich
K
F/mg·L-(1-1/n)·g-1
0.016
0.003
0.0005
n
0.296
0.340
0.381
R
2
0.960
0.920
0.972


### 3.2.3. Adsorption Performance Comparison

Figure2(d) compared the kinetic and isotherm parameters among the three adsorbents. The parameter of K2q2e,cal derived from the pseudo-second-order kinetic model represents the initial adsorption rate [19]. It can be seen that HA possessed the fastest initial adsorption rate then followed by AHA-0.05 and AHA-0.1. The diffusion coefficient of Kid1 from the Weber and Morris intraparticle diffusion model followed the order of HA>AHA−0.05>AHA−0.1. The possible reason is that the formation of CaCO3 deposits on the surface of AHA samples impeded the mass transfer of TC from solution bulk to the outer surface of the adsorbents [20]. The values of Kid1 can also explain why the parameter of K2q2e,cal for HA was significantly bigger than those for AHA-0.05 and AHA-0.1. Based on the values of KF (Freundlich constant), it can be concluded that HA adsorbed more amounts of TC than AHA samples. These results demonstrated that once HA was wrapped by CaCO3, the adsorption capacity towards TC decreased obviously.


## 3.2.1. Adsorption of TC at Different Contact Times

The effect of contact time on the adsorption of TC by HA, AHA-0.05, and AHA-0.1 was evaluated (Figure2(a)), in which AHA-0.05 and AHA-0.1 indicated the concentration of CaCO3 used for wrapping HA was 0.05 mol/L and 0.1 mol/L, respectively. All the adsorption processes were divided into three stages according to the variation trend of qt along with contact time: stage 1 (0-120 min), stage 2 (120-1320 min), and stage 3 (1320-3000 min). The aforementioned three stages corresponded to a rapid increase in qt value, a slight increase in qt value, and an almost unchanged qt value. The equilibrium times were determined at 1320 min, and the equilibrium adsorption capacities (qe) of HA, AHA-0.05, and AHA-0.1 were 2.82, 2.48, and 2.17 mg/g, respectively. The removal efficiency of TC by HA at equilibrium time was more than 90%, obviously higher than it for AHA-0.05 (81%) and AHA-0.1 (71%) (the insert in Figure 2(a)).Figure 2
(a) Effect of contact time on the adsorption of TC in which the initial concentration was 20 mg/L (insert is the removal efficiency of TC under different contact time); (b) ratio ofqt to qe under different contact time; (c) ratio of qt between two different adsorbents; (d) the parameters of different models (model 1: pseudo-second-order model; model 2: Weber and Morris intraparticle diffusion model; model 3: Freundlich model).
(a)
(b)
(c)
(d)As for stage 1 (0-120 min), the ratio of qt at 30 min toqe for AHA-0.1 and AHA-0.05 was 0.82 and 0.84, respectively, while for HA only 0.55 (Figure 2(b)). When the contact time was prolonged to 120 min, the corresponding ratios (qt,120min/qe) were 0.86, 0.87, and 0.98. Interestingly, the value of qt,120min minus qt,30min equaled 0.43 for HA whereas less than 0.04 for the two AHA samples. These results indicated that for AHA most of the adsorption happened in the first 30 min. The possible reason was that the numbers of adsorption sites on the surface of AHA-0.1 and AHA-0.05 were less than those of HA due to the formation of CaCO3 deposits. TC molecular would occupy most of the adsorption sites on the surfaces of AHA-0.1 and AHA-0.05 at the initial 30 min owing to the limited numbers in adsorption sites. Different from AHA samples, HA provided adequate adsorption sites. Therefore, TC may attach to the sites with higher energy and then the sites with lower energy, possibly resulting in a relatively slower increase in the value of qt in the initial 30 min.The values ofqt at different contact times for the three adsorbents were treated by using a pair-wise comparison, and the results are shown in Figure 2(c). The ratios of qt,AHA-0.05/qt,AHA-0.1 were almost maintained at 1.2 during the whole adsorption process from 10 to 2880 min. The ratios of qt,HA/qt,AHA-0.05 and qt,HA/qt,AHA-0.1 increased quickly from 10 to 240 min, in which the values reached the maximum at 240 min (1.3 for the former and 1.5 for the latter). From 240 to 1320 min, the ratios began to decrease and then kept unchanged from 1320 to 2880 min. As qt,HA/qt,AHA-0.05 multiply by qt,AHA-0.05/qt,AHA-0.1 equals to qt,HA/qt,AHA-0.1, as well as qt,AHA-0.05/qt,AHA-0.1 was almost fixed at 1.2, which means that the values of qt,HA/qt,AHA-0.1 were 1.2 times higher than those of qt,HA/qt,AHA-0.05 during the whole adsorption process from 0 to 2880 min. The difference between qt,HA/qt,AHA and qt,AHA-0.05/qt,AHA-0.1 was the biggest at 10 min and then shrank at 30 min, which further proved that most of the adsorption for AHA samples occurred in the initial stage even at the first 10 minutes.


## 3.2.2. Adsorption Kinetics and Isotherm

The pseudo-first-order kinetic model and the pseudo-second-order kinetic model were used and all the parameters were listed in Table1 and Figure 3(a). The pseudo-second-order kinetic model presented a better fit for HA, AHA-0.05, and AHA-0.1 based on the values of R2. Furthermore, the calculated qe values (2.841, 2.488, and 2.197 mg/g for HA, AHA-0.05, and AHA-0.1, respectively) were close to the experiment data (2.82, 2.48, and 2.17 mg/g, respectively). From Table 1, it can be seen that the value of K2q2e,cal for HA was larger than those for AHA-0.05 and AHA-0.1, indicating that the initial adsorption rate of TC by the three adsorbents followed the order of HA>AHA−0.05>AHA−0.1.Table 1
Parameters of the kinetic models for the adsorption of TC by HA and AHA samples.
Models
Parameters
HA
AHA-0.05
AHA-0.1
Pseudo-first-order
qe,exp/mg·g-1
2.82
2.48
2.17
qe,cal/mg·g-1
1.383
1.274
1.139
K1/h-1
0.0005
0.001
0.001
R
2
0.852
0.827
0.831
Pseudo-second-order
K
2/g·mg-1·h-1
0.037
0.02
0.02
qe,cal/mg·g-1
2.841
2.488
2.197
R
2
0.999
0.999
0.992
K
2
q
2
e,cal/mg·g-1·h-1
0.299
0.125
0.096
Weber and Morris intraparticle diffusion
Ki
d1/mg·g-1·h-1/2
0.276
0.041
0.039
I
1/mg·g-1
-0.115
1.493
1.742
R
2
0.944
0.923
—
Ki
d2/mg·g-1·h-1/2
0.0004
0.0085
0.0860
I
2/mg·g-1
2.8100
2.0850
1.7830
R
2
0.831
0.650
—
Boyd’s film-diffusion model
Intercept
-0.702
0.906
0.845
R
2
0.955
0.744
0.648Figure 3
(a) The pseudo-second-order kinetic model fitting the adsorption of TC onto HA, AHA-0.05 and AHA-0.1, respectively (insert is the e Boyd’s film-diffusion model fitting the adsorption of TC onto HA, AHA-0.05 and AHA-0.1, respectively); (b) the Weber and Morris intraparticle diffusion model fitting the adsorption of TC onto HA, AHA-0.05, and AHA-0.1, respectively.
(a)
(b)The Weber and Morris intraparticle diffusion model was used to investigate the rate-limiting step, and all the fitting dates are shown in Table1. As shown in Figure 3(b), it was found that the fitted curves can be separated into two parts distinctly. All the first lines did not pass through the origin, suggesting that the rate-controlling step for the adsorption of TC on HA, AHA-0.05, and, AHA-0.1 proceeded by the boundary layer and intraparticle diffusion simultaneously. The diffusion coefficients of Kid1 for HA (0.276 mg/g.h1/2) were higher than those for AHA-0.05 (0.041 mg/g.h1/2) and AHA-0.1(0.039 mg/g.h1/2), indicating that the deposits of CaCO3 existing on the surface of AHA reduced the mass transfer rate of TC from the aqueous liquid to the external surface of the adsorbent. Fan et al. [23] indicated that when the curve fitted by the Weber and Morris internal particle diffusion model is extended to y-axis, the intercept obtained is proportional to the thickness of the boundary layer. The larger the intercept is, the thicker the boundary layer has. In this study, the values of I1 for HA, AHA-0.05, and AHA-0.1 were -0.115, 1.493, and 1.742 mg/g, respectively, indicating that when HA was coated with a higher concentration of calcium carbonate, more calcium carbonate precipitates accumulated on the surface of AHA.To further determined the real control step of the adsorption process of TC. The Boyd’s film diffusion model was used. Table1 showed the results of adsorption kinetics. If the plot was nonlinear or linear but does not passes through the origin, the adsorption rate was controlled by film-diffusion. If the plot of Bt against time was linear and passed through the origin, intraparticle diffusion controls the rate of mass transfer [23]. Based on the results from Table 1 and the insert of Figure 3(a), although the plot of Bt against time was linear but the intercept of the plots of HA, AHA-0.05, and AHA-0.1, adsorption are -0.702, 0.906, and 0.845, which were significantly different from zero. The intercept of the plots of AHA was farther away from zero, and according to the result of the introparticle diffusion plot, it is indicated that the adsorption rate of TC on AHA was more greatly affected by film diffusion.Freundlich and D-R model fitting results are shown in Figure4, and the fitting parameters are shown in Table 2. For the Freundlich model, the value of R2 for HA, AHA-0.05, and AHA-0.1 was 0.960, 0.920, and 0.972, respectively, while for the Dubinin-Radushkevich model the values of R2 were correspondingly 0.920, 0.970 and 0.900. Adsorption free energy E can be determined from the D-R isotherm, which gives information about the adsorption nature. If the magnitude of E is between 8 and 16 kJ/mol, the sorption process is chemical. If the value of E is less than 8 kJ/mol, the sorption process is supposed to proceed via physical sorption [24]. Based on Table 2, it can be concluded that the adsorption nature of the TC on HA, AHA-0.05, and AHA-0.1 was physisorption.Figure 4
(a) Freundlich model fitting the adsorption of TC on the AHA-0.05 and AHA-0.1 HA, and insert is the HA; (b) Dubinin-Radushkevich model fitting the adsorption of TC on the AHA-0.05 and AHA-0.1, and insert is the HA.
(a)
(b)Table 2
Parameters of the isotherm models describing the sorption of TC.
Model
Parameters
HA
AHA-0.05
AHA-0.1
D-R
E/kJ·Mol-1
2.083
1.730
1.740
qD/mg·g-1
33.115
13.197
8.248
R
2
0.920
0.970
0.900
Freundlich
K
F/mg·L-(1-1/n)·g-1
0.016
0.003
0.0005
n
0.296
0.340
0.381
R
2
0.960
0.920
0.972


## 3.2.3. Adsorption Performance Comparison

Figure2(d) compared the kinetic and isotherm parameters among the three adsorbents. The parameter of K2q2e,cal derived from the pseudo-second-order kinetic model represents the initial adsorption rate [19]. It can be seen that HA possessed the fastest initial adsorption rate then followed by AHA-0.05 and AHA-0.1. The diffusion coefficient of Kid1 from the Weber and Morris intraparticle diffusion model followed the order of HA>AHA−0.05>AHA−0.1. The possible reason is that the formation of CaCO3 deposits on the surface of AHA samples impeded the mass transfer of TC from solution bulk to the outer surface of the adsorbents [20]. The values of Kid1 can also explain why the parameter of K2q2e,cal for HA was significantly bigger than those for AHA-0.05 and AHA-0.1. Based on the values of KF (Freundlich constant), it can be concluded that HA adsorbed more amounts of TC than AHA samples. These results demonstrated that once HA was wrapped by CaCO3, the adsorption capacity towards TC decreased obviously.


## 3.3. Adsorption Mechanism of TC by HA and AHA Samples

### 3.3.1. Morphology Analysis

As shown in Figure5(a), HA, AHA-0.05, and AHA-0.1 samples exhibited type-III adsorption isothermal curves, in which the BET specific surface areas were calculated to be 5, 3, and 2 m2·g-1, respectively. The correspondingly total pore volumes were 0.08, 0.06 and 0.05 cm3·g-1 (P/P0=0.9933) (Figure 5(b)). The decreasing percentages (SHA−SAHA/SHA) of AHA-0.05 to HA and AHA-0.1 to HA in BET specific surface areas were 44% and 50%, respectively. As can be seen from Figure 5(b), the pore sizes of the three adsorbents are mainly concentrated at 30 nm (HA), 28 nm (AHA-0.05), and 20 nm (AHA-0.1), respectively. These results demonstrated that the formation of CaCO3 deposits on the surface of AHA samples rendered them with lower specific surface areas and smaller pore sizes compared to HA, which explains why the HA samples possessed quicker adsorption kinetics and higher adsorption amount.Figure 5
(a) Nitrogen sorption-desorption isotherm for HA, insert for AHA samples; (b) pore size distribution of HA, AHA-0.05 and AHA-0.1.
(a)
(b)


### 3.3.2. Chemical Composition Analysis

In addition to the pore analysis, the morphology and the functional groups of fresh HA and aged HA were compared. The adsorption mechanisms of TC on fresh HA and aged HA were explored. The functional groups of the tree adsorbents were also analyzed by XPS technology to explain the adsorption mechanism from the viewpoint of molecular interaction. As shown in Figure6(a), the full scan survey XPS spectra revealed that HA, AHA-0.05, and AHA-0.1 were mainly composed of C and O as well as small amounts of Si and Al. Interestingly, a new peak of Ca 2p emerged for the two AHA samples, suggesting that calcium carbonate covered their surfaces. Figures 6(b)–6(d) are the XPS high-resolution graphs of C 1 s for HA, AHA-0.05, and, AHA-0.1, in which it was found that three states of carbon existed. It is well known that the C element has four kinds of binding forms. The first one is carbon atom links with carbon or hydrogen atom to form C-C or C-H corresponding to relatively lower binding energy (285 eV) [25]. The second one is the carbon atom connects with the carbonyl oxygen atom to form C-O at 286. 5 eV. The third one is carbon atom binds with two oxygen atoms (O-C-O) or connects with one atom of oxygen to form C=O, in which the energy spectrum peak locate between 288~288.5 eV [26]. The last one is a carbon atom that reacts with two oxygen atoms to generate O−C=O under high binding energy of 289~289.5 eV [26]. Therefore, it can be deduced that HA possessed the functional groups of C-C or C-H (284.8 eV), C-O (286.3 eV), and O-C=O bond (289.1 eV) (Figure 6(b)). Similar conclusions can be used for AHA-0.05 and AHA-0.1 (Figures 6(c) and 6(d)). Furthermore, the content of the three functional groups was calculated, and the results were given in Figure 6(e). It was observed that after being wrapped by CaCO3, the percentage of O−C=O for the two AHA samples decreased obviously compared to HA and followed the order of HA>AHA−0.05>AHA−0.1.Figure 6
(a) XPS full scan spectra of HA and AHA samples; high resolution C1s XPS spectra of (b) HA (c) AHA-0.05, and (d) AHA-0.1; (e) the percentage of different functional groups carried by HA, AHA-0.05 and AHA-0.1.
(a)
(b)
(c)
(d)
(e)The FT-IR spectra of HA before and after adsorption of TC are shown in Figure7(a). The peak at 3416 cm-1 was attributed to the stretching vibration of phenolic hydroxyl or alcohol hydroxyl [27]. The peak at 1572 cm-1 was identified as N-H bending or C-N stretching vibration of amide II [19]. The peak at 1401 cm-1 belonged to the symmetric vibration of carboxylate [28]. A sharp band at 1032 cm-1 was assigned to C-O, which indicated the occurrence of carbohydrates [29]. The sharp peak at 912 cm-1 belonged to the vibration of the aliphatic skeleton [27]. 2D-COS was further used to analyze the FT-IR to sequence the binding affinity of HA towards TC. On the diagonals of the synchronization graph (Figure 7(b)), there are five self-intersecting positive peaks appeared at the main diagonal, as well as five cross-peaks were observed on both sides of the diagonal, indicating that the signal of functional groups on the surface of HA changed along with the change in TC concentrations. In the asynchronous map (Figure 7(c)), six cross-peaks located at 912/1032 cm-1, 912/3416 cm-1, 1032/3416 cm-1, 1401/1572 cm-1, 1572/3416 cm-1, and 1401/3416 cm-1 were positive. Four negative peaks were located at 912/1401 cm-1, 912/1572 cm-1, 1032/1401 cm-1, and 1032/1572 cm-1. Table 3 lists the signs of each cross peak in the synchronous (Ф) and asynchronous (Ψ) maps. According to Noda’s rule [30], it can be concluded that the sequence of the binding affinities of TC was −COO>N−H>−C−C>C−O>O−H, which means that -COO was the most sensitive group.Figure 7
(a) FT-IR spectra of HA sample and HA after adsorbed different concentrations of TC; (b) synchronous and (c) asynchronous 2D correlation maps generated from the FT-IR.
(a)
(b)
(c)Table 3
Signs of each cross peak in the synchronous (Ф) and asynchronous (Ψ) maps.
Position (cm-1)
Sign
912
1032
1401
1572
3416
912
+
+
—
—
+
1032
+
—
—
+
1401
+
+
+
1572
+
+
3416
+
(signs were obtained in the maps of 2D-COS map, + positive and - negative).The analysis result from XPS (Figure6(e)) proved that the content of -COO decreased once HA was wrapped by CaCO3 deposits, in which it followed the order of HA>AHA−0.05>AHA−0.1. As FTIR-2D-COS indicates that -COO was the functional group with the highest affinity towards TC, it explains why HA possessed the biggest adsorption amounts and fastest adsorption kinetics and then followed by AHA-0.05 and AHA-0.1 (Figure 2(d)).


## 3.3.1. Morphology Analysis

As shown in Figure5(a), HA, AHA-0.05, and AHA-0.1 samples exhibited type-III adsorption isothermal curves, in which the BET specific surface areas were calculated to be 5, 3, and 2 m2·g-1, respectively. The correspondingly total pore volumes were 0.08, 0.06 and 0.05 cm3·g-1 (P/P0=0.9933) (Figure 5(b)). The decreasing percentages (SHA−SAHA/SHA) of AHA-0.05 to HA and AHA-0.1 to HA in BET specific surface areas were 44% and 50%, respectively. As can be seen from Figure 5(b), the pore sizes of the three adsorbents are mainly concentrated at 30 nm (HA), 28 nm (AHA-0.05), and 20 nm (AHA-0.1), respectively. These results demonstrated that the formation of CaCO3 deposits on the surface of AHA samples rendered them with lower specific surface areas and smaller pore sizes compared to HA, which explains why the HA samples possessed quicker adsorption kinetics and higher adsorption amount.Figure 5
(a) Nitrogen sorption-desorption isotherm for HA, insert for AHA samples; (b) pore size distribution of HA, AHA-0.05 and AHA-0.1.
(a)
(b)


## 3.3.2. Chemical Composition Analysis

In addition to the pore analysis, the morphology and the functional groups of fresh HA and aged HA were compared. The adsorption mechanisms of TC on fresh HA and aged HA were explored. The functional groups of the tree adsorbents were also analyzed by XPS technology to explain the adsorption mechanism from the viewpoint of molecular interaction. As shown in Figure6(a), the full scan survey XPS spectra revealed that HA, AHA-0.05, and AHA-0.1 were mainly composed of C and O as well as small amounts of Si and Al. Interestingly, a new peak of Ca 2p emerged for the two AHA samples, suggesting that calcium carbonate covered their surfaces. Figures 6(b)–6(d) are the XPS high-resolution graphs of C 1 s for HA, AHA-0.05, and, AHA-0.1, in which it was found that three states of carbon existed. It is well known that the C element has four kinds of binding forms. The first one is carbon atom links with carbon or hydrogen atom to form C-C or C-H corresponding to relatively lower binding energy (285 eV) [25]. The second one is the carbon atom connects with the carbonyl oxygen atom to form C-O at 286. 5 eV. The third one is carbon atom binds with two oxygen atoms (O-C-O) or connects with one atom of oxygen to form C=O, in which the energy spectrum peak locate between 288~288.5 eV [26]. The last one is a carbon atom that reacts with two oxygen atoms to generate O−C=O under high binding energy of 289~289.5 eV [26]. Therefore, it can be deduced that HA possessed the functional groups of C-C or C-H (284.8 eV), C-O (286.3 eV), and O-C=O bond (289.1 eV) (Figure 6(b)). Similar conclusions can be used for AHA-0.05 and AHA-0.1 (Figures 6(c) and 6(d)). Furthermore, the content of the three functional groups was calculated, and the results were given in Figure 6(e). It was observed that after being wrapped by CaCO3, the percentage of O−C=O for the two AHA samples decreased obviously compared to HA and followed the order of HA>AHA−0.05>AHA−0.1.Figure 6
(a) XPS full scan spectra of HA and AHA samples; high resolution C1s XPS spectra of (b) HA (c) AHA-0.05, and (d) AHA-0.1; (e) the percentage of different functional groups carried by HA, AHA-0.05 and AHA-0.1.
(a)
(b)
(c)
(d)
(e)The FT-IR spectra of HA before and after adsorption of TC are shown in Figure7(a). The peak at 3416 cm-1 was attributed to the stretching vibration of phenolic hydroxyl or alcohol hydroxyl [27]. The peak at 1572 cm-1 was identified as N-H bending or C-N stretching vibration of amide II [19]. The peak at 1401 cm-1 belonged to the symmetric vibration of carboxylate [28]. A sharp band at 1032 cm-1 was assigned to C-O, which indicated the occurrence of carbohydrates [29]. The sharp peak at 912 cm-1 belonged to the vibration of the aliphatic skeleton [27]. 2D-COS was further used to analyze the FT-IR to sequence the binding affinity of HA towards TC. On the diagonals of the synchronization graph (Figure 7(b)), there are five self-intersecting positive peaks appeared at the main diagonal, as well as five cross-peaks were observed on both sides of the diagonal, indicating that the signal of functional groups on the surface of HA changed along with the change in TC concentrations. In the asynchronous map (Figure 7(c)), six cross-peaks located at 912/1032 cm-1, 912/3416 cm-1, 1032/3416 cm-1, 1401/1572 cm-1, 1572/3416 cm-1, and 1401/3416 cm-1 were positive. Four negative peaks were located at 912/1401 cm-1, 912/1572 cm-1, 1032/1401 cm-1, and 1032/1572 cm-1. Table 3 lists the signs of each cross peak in the synchronous (Ф) and asynchronous (Ψ) maps. According to Noda’s rule [30], it can be concluded that the sequence of the binding affinities of TC was −COO>N−H>−C−C>C−O>O−H, which means that -COO was the most sensitive group.Figure 7
(a) FT-IR spectra of HA sample and HA after adsorbed different concentrations of TC; (b) synchronous and (c) asynchronous 2D correlation maps generated from the FT-IR.
(a)
(b)
(c)Table 3
Signs of each cross peak in the synchronous (Ф) and asynchronous (Ψ) maps.
Position (cm-1)
Sign
912
1032
1401
1572
3416
912
+
+
—
—
+
1032
+
—
—
+
1401
+
+
+
1572
+
+
3416
+
(signs were obtained in the maps of 2D-COS map, + positive and - negative).The analysis result from XPS (Figure6(e)) proved that the content of -COO decreased once HA was wrapped by CaCO3 deposits, in which it followed the order of HA>AHA−0.05>AHA−0.1. As FTIR-2D-COS indicates that -COO was the functional group with the highest affinity towards TC, it explains why HA possessed the biggest adsorption amounts and fastest adsorption kinetics and then followed by AHA-0.05 and AHA-0.1 (Figure 2(d)).


## 4. Conclusions

The adsorption capacities of TC by HA, AHA-0.05, and AHA-0.1 were evaluated, in which AHA-0.05 and AHA-0.1 indicated that HA was wrapped by 0.05 mol/L and 0.1 mol/L of CaCO3, respectively. The initial adsorption rate (K2q2e,cal) for HA was calculated to be 0.299 mg/(g h), while for AHA-0.05 and AHA-0.1 were 0.125 and 0.096 mg/(g h). The values of diffusion coefficient Kid1 were 0.276, 0.041, and 0.039 mg/(g·h1/2) corresponding to HA, AHA-0.05, and AHA-0.1. Freundlich constant KF for HA, AHA-0.05, and AHA-0.1 was 0.016, 0.003, and 0.0005 mg/(L1-1/n·g), respectively. These results suggested that HA adsorbed more amount of TC at a quicker speed than AHA samples did, demonstrating HA exhibited better adsorption performance towards TC. To explain the aforementioned phenomenon, technologies of BET, XPS, FT-IR, and 2D-COS were adopted. It was found that the specific surface areas of HA, AHA-0.05, and AHA-0.1 were 5, 3, and 2 m2·g-1, respectively. The functional groups of phenolic or alcohol, amide II, carboxylate group, carbohydrates, and aliphatic skeleton carried by HA were proved to be the adsorption sites for extracting TC from aqueous solution. The binding affinity of these groups towards TC was sequenced as −COO>N−H>−C−C>C−O>O−H, suggesting that the carboxylate group played a significant role in the adsorption of TC. The content of the carboxylate group shrunk once HA was wrapped by CaCO3. As AHA samples simulated the aging process, these results demonstrated that aging will affect the physical morphology and chemical composition of HA itself and consequently lead to the decrease in the retention extent of TC in HA.



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*Source: 5362178-2022-10-10.xml*