# Anti-Inflammatory Effects of a Polyphenols-Rich Extract from Tea (Camellia sinensis) Flowers in Acute and Chronic Mice Models

Authors: Bang-Tian Chen; Wei-Xi Li; Rong-Rong He; Yi-Fang Li; Bun Tsoi; Yu-Jia Zhai; Hiroshi Kurihara
Journal: Oxidative Medicine and Cellular Longevity
 (2012)

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## Abstract

While beneficial health properties of tea leaves have been extensively studied, less attention is paid to the flowers of tea. In this study, the anti-inflammatory effects of hot water extract of tea (Camellia sinensis) flowers were investigated. Pharmacological studies found that administration of tea flowers extract (TFE) could effectively inhibit croton oil-induced ear edema and carrageenin-induced paw edema. Furthermore, administration of TFE also protected against Propionibacterium acnes (P. ances) plus lipopolysaccharide-(LPS-) induced liver inflammation by reversing the histologic damage and plasma alanine aminotransferase (ALT) increase. Moreover, the levels of nitric oxide (NO), tumor necrosis factor-(TNF)-α and interleukin-(IL-) 1β mRNA in mouse liver were markedly suppressed after treatment with TFE in mice with immunological liver inflammation. These results indicated that tea flowers had potent anti-inflammatory effects on acute and immunological inflammation in vivo, and may be used as a functional natural food.


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## Body

## 1. Introduction

Tea (Camellia sinensis) is one of the most widely consumed beverages in the world, which contains a large quantity of polyphenols and alkaloids. The unique flavor of tea is contributed by its characteristic polyphenol compounds. Together with phenolic acids such as gallic acid, polyphenols are constituting up to 30% of the dry weight of tea leaves [1]. During the past decades, numerous in vitro and in vivo studies have suggested the biological properties of tea and tea polyphenols in cancer, cardiovascular disease, antioxidant, anti-inflammatory, antiobesity, and neurodegenerative disorders [2–7]. Therefore, polyphenol content has been considered as an important chemical parameter for tea quality regarding its biological property.In our daily life, we enjoy the delightful tea beverages prepared from the leaf buds. There are also several kinds of flower teas in the markets, namely, jasmine, cinnamon, rose, lotus, and chrysanthemum flower teas, which are made from tea leaves and different species of flowers [8]. Interestingly, less attention has been given to flower of tea (Camellia sinensis). In fact, tea flower, as well as tea, has been considered a healthful beverage since ancient times, especially in southern China. Tea flower possess a pleasant bitter taste which persist in the mouth after drinking. It has also been considered and widely used as a folk herb to treat a variety of inflammatory diseases for several centuries. However, less pharmacological studies have been carried out on this herbal medicine. Previous study have demonstrated that tea flower extracts exhibited strong hydroxyl radical scavenging effects in the Fenton reaction system and nitric oxide suppressing effects in LPS-induced Raw 264.7 cells [8]. It was also reported that water extract of tea flowers had potent bioactivity against human breast cancer MCF-7 cells in vitro [9]. Tea flower showed strong antioxidant effects, however, scavenging of free radicals may not be the sole mechanism by which tea-derived polyphenols exert their protective effects. It is noteworthy that most reports on the beneficial effects of tea flowers have been obtained from in vitro studies and more detailed investigations are required to explore in vivo situations. The purpose of this study was to determine the polyphenols content in tea flower. In addition, we also examine the anti-inflammatory effect of a crude tea flower extract on acute inflammation and P. acnes plus LPS-induced liver inflammation in mice.


## 2. Materials and Methods

### 2.1. Materials and Preparation of Tea Flowers Extract (TFE)

Gallic acid (GA), (−)-epicatechin gallate (ECG), (−)-epicatechin (EC), (−)-gallocatechin gallate (GCG), (−)-epigallocatechin (EGC), and (−)-epigallocatechin gallate (EGCG) were purchased from Wako Pure Chemical Ind. (Osaka, Japan). Myricetin, quercetin, kaempferol, croton oil, carrageenin, Evans blue, Cyclosporin A (Cs A), dexamethasone acetate (DEX), and LPS fromEscherichia coli 055:B5 were purchased from Sigma Chemical Co. (St. Louis, Mo, USA). P. acnes (ATCC6919) used in this study had previously been prepared by our laboratory.Tea flowers were procured from Guangdong Tea Experiment Substation, Yingde, Guangdong, China. Dried tea flowers were treated with 20 parts of boiling water for 5 min. After filtration and evaporation of water, the residue was powdered under frozen decompression. The recovery rate was 19.5%. The extract residue was immediately dissolved in water before the experiment.


### 2.2. Polyphenol Compounds Analysis of Tea Flowers

The concentrations of tea catechins in tea flower buds was determined by HPLC under the following conditions: Cosmosil 5PE-MS column (Nakalai Tesuque, Kyoto, Japan, 4.6 mm× 150 mm, 5 μm); mobile phase, eluent A 0.05% trifluoroacetic acid (TFA) in water; eluent B 0.05% TFA in acetonitrile; flow rate, 2 mL/min; column temperature, 40°C; detection wavelength of 280 nm. The analysis was performed with a gradient program of 10% eluent B for 5 min, 21% for 8 min, and 90% for 6 min [10]. The quantification of individual catechins was determined by standard calibration curves calculated from those of authentic reference standards. The total flavonoid content was determined using a colorimetric method as described by Wu et al. [11]. As shown in Table 1, EGCG, ECG, were identified as major polyphenol constituents of tea flower, while GCG and GA were present in smaller quantities. The total catechins and flavonoid contents in tea flowers were 37.46 and 11.61 mg/g, respectively. All the analyses have been performed in triplicate and the results are reported on wet basis.Table 1
Amounts of polyphenols in water extract of tea flowers.
Compounds
mg/g of tea flowers
EGCG
15.45
EC
4.07
GCG
0.43
ECG
11.10
EGC
5.08
GA
1.33
Total flavonoid content
11.61


### 2.3. Experimental Animals

Eight-week-old male KM mice were purchased from the Guangdong Medical Laboratory Animal Center (Guangzhou, China). All mice were kept in a pathogen-free animal room under controlled conditions at23±1°C. A 12 h light-dark cycle was maintained, with lights on from the time of 06:00 to 18:00. The mice were provided with standard laboratory diet and water. The animals were allowed to acclimatize to the environment for 1 week before the experiment. Procedures for animal experiments were conducted in accordance with the Guiding Principles for the Care and Use of Laboratory Animals as adopted and promulgated by the United States National Institutes of Health.


### 2.4. Croton Oil-Induced Edema and Capillary Permeability in Mice Ear

The croton oil ear test was performed according to [12, 13]. A total of 25 μL of 0.3% croton oil (dissolved in acetone) was topically applied to the inner surface of the right ear of each mouse. DEX (1 mg/kg, served as positive control) and TFE (50 mg/kg, 100 mg/kg, and 200 mg/kg) were orally administered to mice 30 min prior to the application of croton oil. The left ear remained untreated as negative control. Three hours after croton oil being applied, 1% Evans blue (dissolved in saline) was injected via the tail vein at a dose of 10 mL/kg body weight. The animals were sacrificed 1 h later and both the treated and untreated ears were collected for further determination. The ears were soaked in 1 mL of 1 M KOH at 37°C for 24 h and 9 mL 0.6 M H3PO4-acetone (5 : 13) was added afterwards. The mixture was then centrifuged at 3000 rpm for 15 min. The absorbance of supernatants was detected at 620 nm [12]. The quantification of Evans blue in mice ear, as a major indicator of capillary permeability was determined by standard calibration curves.


### 2.5. Carrageenan-Induced Paw Edema in Mice

The carrageenan-induced paw edema model in mice was used to study the effect of TFE on acute inflammation [14]. Mice were treated with DEX (1 mg/kg, served as positive control), TFE (50 mg/kg, 100 mg/kg, and 200 mg/kg) or the same volume of saline by oral administration. After 30 min, edema was induced by injecting 10 μL of carrageenan (2.0%, w/v) into subplantar area of the right hind paw. Measurement of paw size was carried out with caliper rule (Digimatic, Mitutoyo 500, Japan) 4 h following carrageenan injection. The extent of the edema was measured by the difference in size between the two hind paws.


### 2.6.P. acnes Plus LPS-Induced Immunological Liver Inflammation

TFE (50 mg/kg, 100 mg/kg, and 200 mg/kg) and Cs A (25 mg/kg, served as positive control) were orally administrated to mice for 7 days, while the normal control group and model group received saline only. Liver inflammation was induced by injecting heat-killedP. acnes in saline (0.2 mg/10 g body weight) via the tail vein 2 d after TFE, Cs A and saline administration. On the seventh day, LPS (5 μg/10 g weight) was injected to all mice intravenously except normal control group. The animals were sacrificed under anesthesia with diethyl ether for liver inflammation analysis 5 h after LPS injection [15]. Blood samples were taken from the hearts of the mice and transferred into a tube containing 2% sodium heparin. The tubes were centrifuged at 5000 rpm for 5 min and supernatants were used for following experiment. Plasma alanine aminotransferase (ALT) activity, which is a marker of hepatocyte injury, was determined by using commercial kits and absorbance was detected with MK3 microplate reader (Labsystems Co., Finland) at 492 nm.


### 2.7. Histological Analysis

For histologic analysis, small sections of fresh liver tissues were immediately fixed in 10% buffered formalin and embedded in paraffin wax. Tissue sections (4μm) were stained with haematoxylin and eosin (H&E) and were examined for liver damage under light microscopy.


### 2.8. Determination of Nitric Oxide (NO) Production

NO concentration in liver tissue was determined by the Griess reaction [16]. Briefly, liver tissues were homogenated in saline, a 40 μL 20% liver homogenate sample was transferred into 96-well microplates, and then 160 μL Griess reagent (1% sulfanilamide in 5% H3PO4 and 0.1% N-(1-naphthyl) ethylenediamine dihydrochloride) was added. The absorbance of the chromophore was read at 540 nm. A standard curve was constructed using known concentrations of sodium nitrite [17].


### 2.9. RNA Extraction and Reverse Transcription-Polymerase Chain Reaction (RT-PCR)

Total RNA was extracted from the liver samples by using Trizol Reagent according to the protocol of the manufacturer (Invitrogen, Carlsbad, CA, USA). The concentration of the extracted RNA was calculated by measuring the optical density at 260 nm. Aliquots of 2μg RNA were transformed to first strand complementary DNA (cDNA) at 42°C for 1 h in a 20 μL reaction mixture containing mouse Moloney leukemia virus reverse transcriptase (Invitrogen, CA, USA) with oligo (dT)15 primers (Tiangen, Beijing, China). PCR amplification was carried out with 1 μL of the resulting cDNA, 12.5 μL of 2× Taq reaction buffer (Tiangen, Beijing, China), 2 μL of dNTP mixture, 1 μM forward primer, 1 μM reverse primer, and 1 μL of Taq polymerase (Tiangen, Beijing) in a total volume of 25 μL. The PCR primers for mouse TNF-α (forward, 5′-GGCGGTGCCTATGTCTC-3′; reverse, 5′-GCAGCCTTGTCCCTTGA-3′), IL-1β (forward, 5′-GCTGGAGAGTGTGGAT-3′; reverse, 5′-CTTGTGAGGTGCTGATG-3′), β-actin (forward, 5′-ACCCAGATCATGTTTGAGACC-3′; reverse, 5′-CATACCCAAGAAGGAAGGCT-3′) were used. The thermocycler conditions were 94°C for 30 s, with an annealing temperature of 60°C for 40 s and an elongation temperature of 72°C for 50 s for the first 30 cycles, followed by an elongation temperature of 72°C for 7 min. After the reaction was completed, the amplified product was removed from the tubes and run on 2% agarose gel and visualized by ethidium bromide staining. The band intensity of ethidium bromide fluorescence was measured using an image analysis system (Bio-Rad, Hercules, CA, USA), then quantified with Quantity One analysis software (Bio-Rad, Hercules, CA, USA), and expressed as the ratio to β-actin.


### 2.10. Statistical Analysis

The data were presented as mean ± S.D. and analyzed statistically by one-way analysis of variance (ANOVA) followed by Dunnett’s significant post-hoc test for pair-wise multiple comparisons. Differences in data of biochemical parameters among the different groups were considered to be statistically significant atP<0.05.


## 2.1. Materials and Preparation of Tea Flowers Extract (TFE)

Gallic acid (GA), (−)-epicatechin gallate (ECG), (−)-epicatechin (EC), (−)-gallocatechin gallate (GCG), (−)-epigallocatechin (EGC), and (−)-epigallocatechin gallate (EGCG) were purchased from Wako Pure Chemical Ind. (Osaka, Japan). Myricetin, quercetin, kaempferol, croton oil, carrageenin, Evans blue, Cyclosporin A (Cs A), dexamethasone acetate (DEX), and LPS fromEscherichia coli 055:B5 were purchased from Sigma Chemical Co. (St. Louis, Mo, USA). P. acnes (ATCC6919) used in this study had previously been prepared by our laboratory.Tea flowers were procured from Guangdong Tea Experiment Substation, Yingde, Guangdong, China. Dried tea flowers were treated with 20 parts of boiling water for 5 min. After filtration and evaporation of water, the residue was powdered under frozen decompression. The recovery rate was 19.5%. The extract residue was immediately dissolved in water before the experiment.


## 2.2. Polyphenol Compounds Analysis of Tea Flowers

The concentrations of tea catechins in tea flower buds was determined by HPLC under the following conditions: Cosmosil 5PE-MS column (Nakalai Tesuque, Kyoto, Japan, 4.6 mm× 150 mm, 5 μm); mobile phase, eluent A 0.05% trifluoroacetic acid (TFA) in water; eluent B 0.05% TFA in acetonitrile; flow rate, 2 mL/min; column temperature, 40°C; detection wavelength of 280 nm. The analysis was performed with a gradient program of 10% eluent B for 5 min, 21% for 8 min, and 90% for 6 min [10]. The quantification of individual catechins was determined by standard calibration curves calculated from those of authentic reference standards. The total flavonoid content was determined using a colorimetric method as described by Wu et al. [11]. As shown in Table 1, EGCG, ECG, were identified as major polyphenol constituents of tea flower, while GCG and GA were present in smaller quantities. The total catechins and flavonoid contents in tea flowers were 37.46 and 11.61 mg/g, respectively. All the analyses have been performed in triplicate and the results are reported on wet basis.Table 1
Amounts of polyphenols in water extract of tea flowers.
Compounds
mg/g of tea flowers
EGCG
15.45
EC
4.07
GCG
0.43
ECG
11.10
EGC
5.08
GA
1.33
Total flavonoid content
11.61


## 2.3. Experimental Animals

Eight-week-old male KM mice were purchased from the Guangdong Medical Laboratory Animal Center (Guangzhou, China). All mice were kept in a pathogen-free animal room under controlled conditions at23±1°C. A 12 h light-dark cycle was maintained, with lights on from the time of 06:00 to 18:00. The mice were provided with standard laboratory diet and water. The animals were allowed to acclimatize to the environment for 1 week before the experiment. Procedures for animal experiments were conducted in accordance with the Guiding Principles for the Care and Use of Laboratory Animals as adopted and promulgated by the United States National Institutes of Health.


## 2.4. Croton Oil-Induced Edema and Capillary Permeability in Mice Ear

The croton oil ear test was performed according to [12, 13]. A total of 25 μL of 0.3% croton oil (dissolved in acetone) was topically applied to the inner surface of the right ear of each mouse. DEX (1 mg/kg, served as positive control) and TFE (50 mg/kg, 100 mg/kg, and 200 mg/kg) were orally administered to mice 30 min prior to the application of croton oil. The left ear remained untreated as negative control. Three hours after croton oil being applied, 1% Evans blue (dissolved in saline) was injected via the tail vein at a dose of 10 mL/kg body weight. The animals were sacrificed 1 h later and both the treated and untreated ears were collected for further determination. The ears were soaked in 1 mL of 1 M KOH at 37°C for 24 h and 9 mL 0.6 M H3PO4-acetone (5 : 13) was added afterwards. The mixture was then centrifuged at 3000 rpm for 15 min. The absorbance of supernatants was detected at 620 nm [12]. The quantification of Evans blue in mice ear, as a major indicator of capillary permeability was determined by standard calibration curves.


## 2.5. Carrageenan-Induced Paw Edema in Mice

The carrageenan-induced paw edema model in mice was used to study the effect of TFE on acute inflammation [14]. Mice were treated with DEX (1 mg/kg, served as positive control), TFE (50 mg/kg, 100 mg/kg, and 200 mg/kg) or the same volume of saline by oral administration. After 30 min, edema was induced by injecting 10 μL of carrageenan (2.0%, w/v) into subplantar area of the right hind paw. Measurement of paw size was carried out with caliper rule (Digimatic, Mitutoyo 500, Japan) 4 h following carrageenan injection. The extent of the edema was measured by the difference in size between the two hind paws.


## 2.6.P. acnes Plus LPS-Induced Immunological Liver Inflammation

TFE (50 mg/kg, 100 mg/kg, and 200 mg/kg) and Cs A (25 mg/kg, served as positive control) were orally administrated to mice for 7 days, while the normal control group and model group received saline only. Liver inflammation was induced by injecting heat-killedP. acnes in saline (0.2 mg/10 g body weight) via the tail vein 2 d after TFE, Cs A and saline administration. On the seventh day, LPS (5 μg/10 g weight) was injected to all mice intravenously except normal control group. The animals were sacrificed under anesthesia with diethyl ether for liver inflammation analysis 5 h after LPS injection [15]. Blood samples were taken from the hearts of the mice and transferred into a tube containing 2% sodium heparin. The tubes were centrifuged at 5000 rpm for 5 min and supernatants were used for following experiment. Plasma alanine aminotransferase (ALT) activity, which is a marker of hepatocyte injury, was determined by using commercial kits and absorbance was detected with MK3 microplate reader (Labsystems Co., Finland) at 492 nm.


## 2.7. Histological Analysis

For histologic analysis, small sections of fresh liver tissues were immediately fixed in 10% buffered formalin and embedded in paraffin wax. Tissue sections (4μm) were stained with haematoxylin and eosin (H&E) and were examined for liver damage under light microscopy.


## 2.8. Determination of Nitric Oxide (NO) Production

NO concentration in liver tissue was determined by the Griess reaction [16]. Briefly, liver tissues were homogenated in saline, a 40 μL 20% liver homogenate sample was transferred into 96-well microplates, and then 160 μL Griess reagent (1% sulfanilamide in 5% H3PO4 and 0.1% N-(1-naphthyl) ethylenediamine dihydrochloride) was added. The absorbance of the chromophore was read at 540 nm. A standard curve was constructed using known concentrations of sodium nitrite [17].


## 2.9. RNA Extraction and Reverse Transcription-Polymerase Chain Reaction (RT-PCR)

Total RNA was extracted from the liver samples by using Trizol Reagent according to the protocol of the manufacturer (Invitrogen, Carlsbad, CA, USA). The concentration of the extracted RNA was calculated by measuring the optical density at 260 nm. Aliquots of 2μg RNA were transformed to first strand complementary DNA (cDNA) at 42°C for 1 h in a 20 μL reaction mixture containing mouse Moloney leukemia virus reverse transcriptase (Invitrogen, CA, USA) with oligo (dT)15 primers (Tiangen, Beijing, China). PCR amplification was carried out with 1 μL of the resulting cDNA, 12.5 μL of 2× Taq reaction buffer (Tiangen, Beijing, China), 2 μL of dNTP mixture, 1 μM forward primer, 1 μM reverse primer, and 1 μL of Taq polymerase (Tiangen, Beijing) in a total volume of 25 μL. The PCR primers for mouse TNF-α (forward, 5′-GGCGGTGCCTATGTCTC-3′; reverse, 5′-GCAGCCTTGTCCCTTGA-3′), IL-1β (forward, 5′-GCTGGAGAGTGTGGAT-3′; reverse, 5′-CTTGTGAGGTGCTGATG-3′), β-actin (forward, 5′-ACCCAGATCATGTTTGAGACC-3′; reverse, 5′-CATACCCAAGAAGGAAGGCT-3′) were used. The thermocycler conditions were 94°C for 30 s, with an annealing temperature of 60°C for 40 s and an elongation temperature of 72°C for 50 s for the first 30 cycles, followed by an elongation temperature of 72°C for 7 min. After the reaction was completed, the amplified product was removed from the tubes and run on 2% agarose gel and visualized by ethidium bromide staining. The band intensity of ethidium bromide fluorescence was measured using an image analysis system (Bio-Rad, Hercules, CA, USA), then quantified with Quantity One analysis software (Bio-Rad, Hercules, CA, USA), and expressed as the ratio to β-actin.


## 2.10. Statistical Analysis

The data were presented as mean ± S.D. and analyzed statistically by one-way analysis of variance (ANOVA) followed by Dunnett’s significant post-hoc test for pair-wise multiple comparisons. Differences in data of biochemical parameters among the different groups were considered to be statistically significant atP<0.05.


## 3. Results

### 3.1. Effects of TFE on Croton Oil-Induced Ear Edema and Capillary Permeability in Mice

Topical application of croton oil-induced edema at the ears of mice and significant increase in the weight of treated right ear when compared with the untreated left ear. As shown in Table2, the oral administration of TFE produced a dose-dependent suppression of ear edema in mice. The edema inhibitory rates of TFE were 5.65%, 13.04%, and 25.40% at the dose of 50, 100, and 200 mg/kg, respectively. Whereas DEX (1 mg/kg) gave rise to a significant inhibition of 90.79% in ear weight compared to control. Croton oil-induced capillary permeability leaded to the increase of Evans blue leakage in the mice ear. TFE at doses of 100 and 200 mg/kg showed significant inhibitory effects on croton oil-induced capillary permeability (Table 2), with 21.76% and 25.13% inhibition of dye leakage at doses of 100 mg/kg and 200 mg/kg, respectively. DEX (1 mg/kg) gave a significant inhibition of dye leakage at 90.16%.Table 2
Effects of TFE on croton oil-induced ear edema and capillary permeability in micea.
Group
Dose (mg/kg)
Edema degree (mg)
Evans blue (μg/ear)
control
—
21.9
3
±
2.3
2
3.86
±
0.37
TFE
50
20
.
69
±
3
.
16
3.72
±
0.34
100
19.07 ± 2.04b
3.02 ± 0.26c
200
16.36 ± 1.42c
2.89 ± 0.29c
DEX
1
2.02 ± 0.57c
0.38 ± 0.24c
V
aalues are mean ± S.D. of differences in weight between right and left ear of mice (n=10). bSignificantly different from the control mice at P<0.05. cSignificantly different from the control mice at P<0.01.


### 3.2. Effect of TFE on Carrageenan-Induced Paw Edema in Mice

In the carrageenan-induced edema test, the paw sizes and percentages of inhibition by TFE and DEX are shown in Table3. A maximum increase of paw thickness, 1.22±0.15 mm, was obtained 4 h following carrageenan injection in control mice. TFE significantly decreased the carrageenan-induced edema at doses of 200 mg/kg and achieved its maximal inhibitory effects of 19.67%. At a dose of 1 mg/kg, DEX produced a greater inhibition of edema by 69.67%.Table 3
Effect of TFE on carrageenan-induced paw edema in micea.
Group
Dose (mg/kg)
Paw thickness (mm)
Inhibitory rate (%)
control
—
1.22
±
0.15
—
TFE
50
1.16
±
0.24
4.92
100
1.07
±
0.23
12.30
200
0.98 ± 0.20b
19.67
DEX
1
0.37 ± 0.13b
69.67
V
aalues are mean ± S.D. of differences in thickness between right and left hind paw of mice (n=10). bSignificantly different from the control mice at P<0.01.


### 3.3. Effects of TFE onP. acnes Plus LPS-Induced Liver Inflammation

The effect of TFE onP. acnes plus LPS-induced liver inflammation was investigated by the histologic analysis and plasma ALT level. As shown in Figure 1, there was no obvious pathological abnormality in normal control group, liver parenchyma was in good morphology and hepatocytes were arranged around the central vein, no congestion and inflammation were observed in the sinusoids (Figure 1(a)). Compared with normal mice, liver tissue sections from P. acnes plus LPS challenged mice showed severe pathological alterations, such as confusion of hepatic lobule structure with inflammatory cell infiltration, serious vacuolation of hepatic cells, necrosis and erythrocyte influx (Figure 1(b)). However, in TFE (50, 100, and 200 mg/kg) treated groups and Cs A group, the area and extent of necrosis were attenuated and the immigration of inflammatory cells was reduced (Figures 1(c)–1(f)). As shown in Figure 2, plasma ALT activity in P. acnes plus LPS-treated mice was significantly higher than the normal group (P<0.01). TFE significantly reversed the ALT activity by 16.49%, 46.05%, and 49.42% at doses of 50 mg/kg, 100 mg/kg, and 200 mg/kg, respectively. These data indicate that TFE attenuates liver inflammation induced by P. acnes plus LPS injection.Effects of TFE on histological manifestation inP. acnes plus LPS-induced liver inflammation. Hematoxylin and eosin staining images of representative liver tissue sections were shown at the same magnification (200×). (a) Normal control, (b) liver inflammation model, (c) TFE 50 mg/kg, (d) TFE 100 mg/kg, (e) TFE 200 mg/kg, and (f) Cs A 25 mg/kg.
(a)
(b)
(c)
(d)
(e)
(f)Figure 2
Effects of TFE on plasma ALT activity in mice treated withP. acnes plus LPS. Mice were treated with TFE, Cs A or saline for 7 days. Liver inflammation was induced by P. acnes and LPS injected intravenously on the 2nd and 7th day of TFE administration. The results represented mean ± S.D. of values obtained from 7 mice in each group, with significance showed as P**<0.01 compared with normal group and P#<0.05 and P##<0.01 compared with model group.


### 3.4. Effects of TFE on NO Production inP. acnes Plus LPS Treated Mice

NO is a common inflammatory mediator involved in the pathogenesis of inflammation. In this study, the effect of TFE onP. acnes plus LPS induced liver inflammation was also investigated by determining the NO production in liver tissues. As shown in Figure 3, compared with the normal control mice, the concentration of NO was markedly increased in model group (P<0.01). However, significant concentration-dependent inhibition of NO production was detected when mice were treated with TFE (100, 200 mg/kg), with inhibitory of 28.6% and 34.9%, respectively.Figure 3
Effects of TFE on NO production in livers of mice treated withP. acnes plus LPS. The results represented mean ± S.D. of values obtained from 7 mice in each group, with significance showed as P**<0.01 compared with normal group and P#<0.05 and P##<0.01 compared with model group.


### 3.5. Effects of TFE on TNF-α and IL-1β mRNA Expression

To further explore the mechanisms of the protective effects of TFE on immunological liver inflammation induced byP. acnes and LPS, mRNA expression of TNF-α and IL-1β mRNA in liver tissue were determined. Compared with the normal control group, treatment with P. acnes and LPS led to significant increases in TNF-α and IL-1β mRNA levels (Figure 4). However, this effect was reversed in mice treated with TFE (50, 100, and 200 mg/kg), with similar result from mice treated with Cs A.Effects of TFE on TNF-α and IL-1β mRNA expression. (a) Agarose gel electrophoresis of RT-PCR amplification of TNF-α, IL-1β and β-actin mRNA. (b) Densitometric analysis of PCR products of TNF-α and IL-1β mRNA. The results represented mean ± S.D. of values obtained from 7 mice in each group, with significance showed as P**<0.01 compared with normal group and P#<0.05 and P##<0.01 compared with model group.
(a)
(b)


## 3.1. Effects of TFE on Croton Oil-Induced Ear Edema and Capillary Permeability in Mice

Topical application of croton oil-induced edema at the ears of mice and significant increase in the weight of treated right ear when compared with the untreated left ear. As shown in Table2, the oral administration of TFE produced a dose-dependent suppression of ear edema in mice. The edema inhibitory rates of TFE were 5.65%, 13.04%, and 25.40% at the dose of 50, 100, and 200 mg/kg, respectively. Whereas DEX (1 mg/kg) gave rise to a significant inhibition of 90.79% in ear weight compared to control. Croton oil-induced capillary permeability leaded to the increase of Evans blue leakage in the mice ear. TFE at doses of 100 and 200 mg/kg showed significant inhibitory effects on croton oil-induced capillary permeability (Table 2), with 21.76% and 25.13% inhibition of dye leakage at doses of 100 mg/kg and 200 mg/kg, respectively. DEX (1 mg/kg) gave a significant inhibition of dye leakage at 90.16%.Table 2
Effects of TFE on croton oil-induced ear edema and capillary permeability in micea.
Group
Dose (mg/kg)
Edema degree (mg)
Evans blue (μg/ear)
control
—
21.9
3
±
2.3
2
3.86
±
0.37
TFE
50
20
.
69
±
3
.
16
3.72
±
0.34
100
19.07 ± 2.04b
3.02 ± 0.26c
200
16.36 ± 1.42c
2.89 ± 0.29c
DEX
1
2.02 ± 0.57c
0.38 ± 0.24c
V
aalues are mean ± S.D. of differences in weight between right and left ear of mice (n=10). bSignificantly different from the control mice at P<0.05. cSignificantly different from the control mice at P<0.01.


## 3.2. Effect of TFE on Carrageenan-Induced Paw Edema in Mice

In the carrageenan-induced edema test, the paw sizes and percentages of inhibition by TFE and DEX are shown in Table3. A maximum increase of paw thickness, 1.22±0.15 mm, was obtained 4 h following carrageenan injection in control mice. TFE significantly decreased the carrageenan-induced edema at doses of 200 mg/kg and achieved its maximal inhibitory effects of 19.67%. At a dose of 1 mg/kg, DEX produced a greater inhibition of edema by 69.67%.Table 3
Effect of TFE on carrageenan-induced paw edema in micea.
Group
Dose (mg/kg)
Paw thickness (mm)
Inhibitory rate (%)
control
—
1.22
±
0.15
—
TFE
50
1.16
±
0.24
4.92
100
1.07
±
0.23
12.30
200
0.98 ± 0.20b
19.67
DEX
1
0.37 ± 0.13b
69.67
V
aalues are mean ± S.D. of differences in thickness between right and left hind paw of mice (n=10). bSignificantly different from the control mice at P<0.01.


## 3.3. Effects of TFE onP. acnes Plus LPS-Induced Liver Inflammation

The effect of TFE onP. acnes plus LPS-induced liver inflammation was investigated by the histologic analysis and plasma ALT level. As shown in Figure 1, there was no obvious pathological abnormality in normal control group, liver parenchyma was in good morphology and hepatocytes were arranged around the central vein, no congestion and inflammation were observed in the sinusoids (Figure 1(a)). Compared with normal mice, liver tissue sections from P. acnes plus LPS challenged mice showed severe pathological alterations, such as confusion of hepatic lobule structure with inflammatory cell infiltration, serious vacuolation of hepatic cells, necrosis and erythrocyte influx (Figure 1(b)). However, in TFE (50, 100, and 200 mg/kg) treated groups and Cs A group, the area and extent of necrosis were attenuated and the immigration of inflammatory cells was reduced (Figures 1(c)–1(f)). As shown in Figure 2, plasma ALT activity in P. acnes plus LPS-treated mice was significantly higher than the normal group (P<0.01). TFE significantly reversed the ALT activity by 16.49%, 46.05%, and 49.42% at doses of 50 mg/kg, 100 mg/kg, and 200 mg/kg, respectively. These data indicate that TFE attenuates liver inflammation induced by P. acnes plus LPS injection.Effects of TFE on histological manifestation inP. acnes plus LPS-induced liver inflammation. Hematoxylin and eosin staining images of representative liver tissue sections were shown at the same magnification (200×). (a) Normal control, (b) liver inflammation model, (c) TFE 50 mg/kg, (d) TFE 100 mg/kg, (e) TFE 200 mg/kg, and (f) Cs A 25 mg/kg.
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(f)Figure 2
Effects of TFE on plasma ALT activity in mice treated withP. acnes plus LPS. Mice were treated with TFE, Cs A or saline for 7 days. Liver inflammation was induced by P. acnes and LPS injected intravenously on the 2nd and 7th day of TFE administration. The results represented mean ± S.D. of values obtained from 7 mice in each group, with significance showed as P**<0.01 compared with normal group and P#<0.05 and P##<0.01 compared with model group.


## 3.4. Effects of TFE on NO Production inP. acnes Plus LPS Treated Mice

NO is a common inflammatory mediator involved in the pathogenesis of inflammation. In this study, the effect of TFE onP. acnes plus LPS induced liver inflammation was also investigated by determining the NO production in liver tissues. As shown in Figure 3, compared with the normal control mice, the concentration of NO was markedly increased in model group (P<0.01). However, significant concentration-dependent inhibition of NO production was detected when mice were treated with TFE (100, 200 mg/kg), with inhibitory of 28.6% and 34.9%, respectively.Figure 3
Effects of TFE on NO production in livers of mice treated withP. acnes plus LPS. The results represented mean ± S.D. of values obtained from 7 mice in each group, with significance showed as P**<0.01 compared with normal group and P#<0.05 and P##<0.01 compared with model group.


## 3.5. Effects of TFE on TNF-α and IL-1β mRNA Expression

To further explore the mechanisms of the protective effects of TFE on immunological liver inflammation induced byP. acnes and LPS, mRNA expression of TNF-α and IL-1β mRNA in liver tissue were determined. Compared with the normal control group, treatment with P. acnes and LPS led to significant increases in TNF-α and IL-1β mRNA levels (Figure 4). However, this effect was reversed in mice treated with TFE (50, 100, and 200 mg/kg), with similar result from mice treated with Cs A.Effects of TFE on TNF-α and IL-1β mRNA expression. (a) Agarose gel electrophoresis of RT-PCR amplification of TNF-α, IL-1β and β-actin mRNA. (b) Densitometric analysis of PCR products of TNF-α and IL-1β mRNA. The results represented mean ± S.D. of values obtained from 7 mice in each group, with significance showed as P**<0.01 compared with normal group and P#<0.05 and P##<0.01 compared with model group.
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## 4. Discussion

Several experimental and epidemiological studies have related tea consumption with various biological and pharmacological functions [18, 19]. It is believed that the health benefit of tea are mainly attributed to its antioxidant properties and the ability of its polyphenolic catechins to scavenge reactive oxygen species [20]. In this study, the contents of six catechins, namely, EGCG, EC, GCG, ECG, EGC, and GA, in tea flowers were detected using HPLC. In addition, total flavonoid content was determined; flavonols were reported to contribute importantly to antioxidant activity and the color of tea [21]. The present study demonstrated that tea flowers contain appreciable amounts of polyphenols. These results indicated that tea flowers might exhibit beneficial health properties and might be suitable for making an alternative to tea beverage.In this study, the anti-inflammatory effects of TFE were evaluated by various approaches in mice model. Ear edema induced by croton oil has been widely accepted as a useful pharmacological model for the investigation of new anti-inflammatory drugs [22]. Croton oil contains 12-O-tetradecanoylphorbol-13-acetate (TPA) and other phorbol esters as main irritant agents [23]. Application of croton oil can induce significant inflammatory responses as characterized by edema, neutrophil infiltration, prostaglandins production, and increases in vascular permeability [24]. It is reported that cyclooxygenase inhibitors, 5-lipoxygenase inhibitors are highly effective against inflammation caused by TPA [25]. Polyphenols have already been recognized as potent inhibitors of cyclooxygenase and lipoxygenase in different studies [26–28]. Thus, results obtained in this research suggested that TFE may have similar pharmacological properties with those of cyclooxygenase and lipoxygenase inhibitors, which might be related to the containing polyphenols. Carrageenan-induced paw edema assay was also carried out to estimate the effect of TFE on acute inflammation induced by a different irritant. According to our results, TFE (200 mg/kg) also showed significant effect against carrageenan-induced paw edema in mice model. From these two results, it is believed that TFE has potent anti-inflammatory effects in mice with different irritate agents.The effect of TFE onP. acnes plus LPS-induced liver inflammation was investigated as well. Liver inflammation evoked by intravenous injection of P. acnes plus LPS had been considered as experimentally induced chronic autoimmune active hepatitis [29, 30]. Which can be indicated by the elevation of plasma ALT level [15]. When the mice were treated with TFE (50, 100, and 200 mg/kg), the elevated plasma ALT activity was significantly decreased. We also found that P. acnes plus LPS injection increased the levels of NO in liver. During inflammation, reactive oxygen species (ROS) produce high levels of NO to exert a defense against pathogens, but excess NO production is believed to act as a toxic radical, which was implicated in the etiology of inflammation and triggering several unfavorable responses [31]. ROS together with NO, a reactive nitrogen species which is known to be involved in oxidative stress, will increase the production of proinflammatory cytokines such as TNF-α and IL-1 [32]. Elevated pro-inflammatory cytokines have been shown to play important pathogenic roles in liver inflammation [33]. In this study, TFE decreased the expressions of TNF-α and IL-1β, these results suggested the protective effect of TFE on immunological liver inflammation by inhibiting the pro-inflammatory cytokines expression.In conclusion, our results clearly indicated that tea flowers are rich in polyphenols and have potent anti-inflammatory effects on acute inflammation and immunological liver inflammationin vivo. Mechanism studies suggested that the anti-inflammatory effects of TFE were associated with the suppression of NO production and the expression of TNF-α and IL-1β mRNA. These findings would be helpful to promote tea flower as a well-accepted healthy tea beverage.



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*Source: 537923-2012-07-30.xml*