# Epithelium-Specific Ets-Like Transcription Factor 1, ESE-1, Regulates ICAM-1 Expression in Cultured Lung Epithelial Cell Lines

Authors: Zhiqi Yu; Jun Xu; Jinbao Liu; Jing Wu; Chan Mi Lee; Li Yu; Jim Hu
Journal: Mediators of Inflammation
 (2015)

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## Abstract

Cystic fibrosis (CF) patients suffer from chronic airway inflammation with excessive neutrophil infiltration. Migration of neutrophils to the lung requires chemokine and cytokine signaling as well as cell adhesion molecules, such as intercellular adhesion molecule-1 (ICAM-1), which plays an important role in mediating adhesive interactions between effector and target cells in the immune system. In this study, we investigated the relationship between ICAM-1 and epithelium-specific ETS-like transcription factor 1 (ESE-1) and found that ICAM-1 expression is upregulated in cell lines of CF (IB3-1) as well as non-CF (BEAS-2B and A549) epithelial origin in response to inflammatory cytokine stimulation. Since ESE-1 is highly expressed in A549 cells without stimulation, we examined the effect of ESE-1 knockdown on ICAM-1 expression in these cells. We found that ICAM-1 expression was downregulated when ESE-1 was knocked down in A549 cells. We also tested the effect of ESE-1 knockdown on cell-cell interactions and demonstrate that the knocking down ESE-1 in A549 cells reduce their interactions with HL-60 cells (human promyelocytic leukemia cell line). These results suggest that ESE-1 may play a role in regulating airway inflammation by regulating ICAM-1 expression.


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## Body

## 1. Introduction

Airway inflammation is a hallmark of the cystic fibrosis (CF) lung disease. The airways of CF patients are initially colonized by viruses, fungi, or bacteria, includingStaphylococcus aureus,Haemophilus influenzae, andKlebsiella pneumonia [1]. Most patients later become infected with mucoid strains ofPseudomonas aeruginosa and some withBurkholderia cepacia [2].In CF patients, the number of neutrophils and the levels of cytokines such as tumor necrosis factor-α (TNF-α), interleukin- (IL-) 6, and IL-8 in the airways are increased compared to non-CF individuals [3, 4]. Cultured CF lung epithelial cells (IB3-1) show downregulation of the anti-inflammatory cytokine IL-10 and an exaggerated upregulation of IL-8 in response to a variety of external stimuli, such as TNF-α and bacterial products [5, 6]. Overproduction of IL-8 is likely a major cause of excessive neutrophil infiltration, since IL-8 is a potent chemoattractant for neutrophils [7].Neutrophil migration in response to inflammatory stimuli requires cell adhesion molecules, such as intercellular adhesion molecule-1 (ICAM-1, also known as CD54) [8, 9]. Migration of neutrophils out of the vascular system occurs in distinct phases: rolling, firm adhesion, and transmigration [10]. Four types of cell adhesion molecules are involved in this process, namely, E-selectin,ICAM-1, vascular cell adhesion molecule-1 (VCAM-1), and platelet-endothelial cell adhesion molecule-1 (PECAM-1). Neutrophil rolling is the first step of the migration process and E-selectin is the key molecule involved in slowing down the circulating neutrophils. This step is critical to ensure firm adhesion of neutrophils to the endothelial cell layer. Firm adhesion is mediated throughICAM-1 expressed on endothelial cells, which interacts with CD11a/CD18 (LFA1) or CD11b/CD18 (Mac-1) as counter-receptors on neutrophils [11]. The final phase of transmigration of neutrophils through the endothelium is triggered by PECAM-1 and VCAM-1 [10]. Currently, the mechanism by which neutrophils migrate to the airway lumen is unclear, but they are thought to travel through the intercellular space [12, 13]. Other cell adhesion molecules such as ICAM-2 and ICAM-3 are also involved in the migration of monocytes [14] or dendritic cells [15].ICAM-1 is a 114 kD inducible surface glycoprotein that belongs to the immunoglobulin superfamily [9] and it plays an important role in innate and adaptive immune responses [16]. Although the role ofICAM-1 in endothelial cells as well as in adaptive immunity [17–20] is well established, the function of epithelialICAM-1 during inflammation is not fully understood. Since epithelialICAM-1 is expressed on the airway lumen [21–24], a role for leukocyte transmigration is not expected. On the other hand, cell adhesion studies [25, 26] indicate that epithelialICAM-1 is important for leukocyte homing. Because neutrophils and macrophages are enriched at the sites of injury or inflammation, it is possible that homing of these cells is part of the resolution of inflammation.Among the adhesion molecules,ICAM-1 may play a more important role in the infiltration of leukocytes during airway inflammation. For example, Hubeau et al. performed quantitative analysis of inflammatory cells infiltrating the CF airway mucosa in lung tissues collected at the time of transplantation and found thatICAM-1, but not VCAM-1 or E-selectin, was overexpressed on the epithelium surface [27]. In addition, a recentin vitro study also showed thatICAM-1 is expressed in a higher percentage of cultured airway epithelial cell lines (IB3-1, C38 and BEAS-2B) than other cell adhesion molecules, such as VCAM-1 or E-selectin [28].ICAM-1 is expressed at a very low level in airway epithelial cells. Interestingly, CF-deficient airway epithelial cells have a slightly higher basal level ofICAM-1 expression [28]. Upon stimulation with proinflammatory cytokine (e.g., TNF-α or IL-1β) [29, 30] or other stimulatory substances (e.g., LPS) [31],ICAM-1 expression is significantly induced in both human primary bronchial epithelial cultures and epithelial cell lines. This induction is mediated by activation of nuclear factor-kappa B (NF-κB) signaling transduction pathway. In addition,ICAM-1 induction can also be mediated through the STAT signaling pathway since IFN-gamma can significantly elevate its expression in epithelial cells [16]. CF airways have chronic inflammation, which contributes to the overexpression ofICAM-1 [27]. Since epithelialICAM-1 may be critical for neutrophil homing and epithelial killing, it is important to understand its regulation and function in airway epithelial cells in order to identify potential drug targets for the CF lung disease.The E26 transformation-specific (ETS) family of transcription factors is characterized by a highly conserved 85 amino acid DNA binding domain, which is known as the ETS domain [32]. It is comprised of 27 and 26 members in humans and mice, respectively. The ETS domain is usually located in the carboxyl-terminal region of the protein as a winged helix-turn-helix structural motif and binds to purine-rich DNA that has a core consensus sequence of GGAA/T- within the promoter and enhancer regions of target genes [33]. ETS transcriptional factors act as both positive and negative regulators of gene expression in various biological processes, such as cellular proliferation, differentiation, apoptosis, metastasis, hematopoiesis, and angiogenesis [34]. Although many of ETS family members are expressed in nonepithelial cells, such as hematopoietic and endothelial cells,ESE-1 is mainly expressed in epithelial-rich tissues, such as lung, kidney, stomach, small intestine, colon, pancreas, trachea, salivary gland, prostate gland, mammary gland, uterus, and skin [35], but it can be upregulated in nonepithelial cells by proinflammatory cytokines such as TNF-α and IL-1β. Our previous work has shown thatESE-1 can be highly induced in epithelial cells by inflammatory cytokines [36].In this study, we investigated the regulation ofICAM-1 expression byESE-1. Here we demonstrate that the expression of bothICAM-1 andESE-1 is upregulated in human bronchial epithelial cells (BEAS2B), CF cells (IB3-1), and lung cancer cells (A549) by inflammatory cytokines. We also show thatICAM-1 expression is downregulated uponESE-1 knockdown in A549 cells and thatESE-1 regulates theICAM-1 expression at the transcriptional level. Finally, we demonstrate that the downregulation ofICAM-1 by knocking downESE-1 in A549 cells results in a reduced capacity of A549 cells to interact with HL-60 cells.


## 2. Materials and Methods

### 2.1. Cell Culture and Reagents

Human bronchial epithelial cells (BEAS-2B), human lung carcinoma cells (A549), and CF bronchi epithelial cells (IB3-1) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) medium (Invitrogen) supplemented with 10% fetal bovine serum (FBS). Recombinant human tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) were obtained from R&D Systems (Minneapolis, MN) and reconstituted in phosphate buffered saline (PBS) containing 0.1% bovine serum albumin (BSA). For cytokines stimulation, TNF-α and IL-1β were used at 10 ng/mL each.


### 2.2. Viral Transduction and shRNA-Mediated Gene Knocking Down

ESE-1 gene was knocked down in A549 cells using a shRNA helper-dependent adenoviral vector expressing two shRNAs from murine U6 gene promoter as described previously [36]. A C4HSU empty vector was used as control. For viral transduction, A549 cells were seeded at 2 × 105 cells per well in 6-well plates overnight, and then cells were transduced at 40–60% confluency with viral vector at 2500 particles/cell (or 50 moi) under serum-free conditions for two hours, followed by addition of media to a final concentration of 10% FBS. We usually achieved near 100% transduction at this vector concentration. Cells were then collected for protein extraction or RNA isolation at desired time points.


### 2.3. Transient Transfection and Luciferase Reporter Assay

For cell transfection and cotransfection experiments, BEAS-2B cells were seeded in 6-well plates and transfected at 50–60% confluency by using PolyFect transfection reagent (Qiagen) according to the manufacturer’s protocol. After 24 h, cells were harvested and luciferase activity was measured using a Dual Lucifcerase kit (Promega) and a luminometer (EG&G Berthold, BadWildbad, Germany) as described previously [37].


### 2.4. RNA Analysis

Cells were lysated and RNAs were extracted by using RNA spin Mini kit (GE Healthcare) according to manufacturer’s instructions. Total RNA (1μg) was reverse transcribed using random hexamers and SuperScript II reverse transcriptase (Invitrogen), and then the resulting template (20 ng cDNA) was used for each real-time PCR reaction (ABI Prism 7700, Applied Biosystems). Primers for humanESE-1 andICAM-1 were purchased from ACGT, Toronto. For relative quantification, PCR signals were compared between groups after normalization with GAPDH as an internal reference. Fold changes were calculated according to Livak and Schmittgen [38]. Primers used were hICAM1-F: GGAAGGTGTATGAACTGAGCAA, hICAM1-R: GGAGTCCAGTACACGGTGA, hese1-F: GGCGTCTTCAAGTTCCTGCG, hese1-R: CTCCCGTTTGTAGTAGTACCT, hGAPDH-F: GAAGGTGAAGGTCGGAGTC, hGAPDH-R: GAAGATGGTGATGGGATTTC.


### 2.5. Western Blotting Analysis

Cell lysates were run on an 8% SDS-polyacrylamide gel at 10μg each lane and transferred to nitrocellulose membrane (Bio-Rad) following electrophoresis. The membrane was then blocked with Tris-buffered saline with Tween-20 (TBST) containing 5% milk and probed with goat anti-humanICAM-1 antibody (R&D System, Minneapolis, USA) at 1 : 1000 dilution and rabbit anti-humanESE-1 antibody (R&D System, Minneapolis, USA) at 1 : 5000 dilution. Rabbit anti-human GAPDH antibodies (Trevigen, Gaithersburg, USA) were used at 1 : 3000 as a protein loading control. The horseradish peroxidase-conjugated secondary antibodies for rabbit anti-goat IgG were from Kirkegaard & Perry Laboratories and for goat anti-rabbit IgG were from Bio-Rad Laboratories. Detection of proteins was performed with enhanced chemiluminescence reagents (Amersham Pharmacia Biotech, Baie-d’Urfe, CA).


### 2.6. Cell Adhesion Assay

At day 0, 1 × 106 A549 cells were seeded on a 10 cm dish and virus vector particles were added at 100 moi (5000 particles per cell) for 1 hour in serum-free media. Fetal bovine serum was then added to final concentration of 10%. At day 5, the treated cells were harvested and seeded at 250,000 to each well of a-24-well plate. The reason for waiting for 5 days was to minimize the influence of inflammatory cytokines induced by the viral transduction. For visualization of cell-cell interactions, HL-60 cells were labeled with carboxyfluorescein succinimide ester (CFSE) at 5 μM in the cell culture for 5 min at 37°C and then washed 3 times with RPMI 1640. The labeled HL-60 cells were put back in the cell culture condition for 24 hours. The A549 cells were then stimulated with IL-1β and TNF-α each at 10 ng/mL for 6 hours and labeled HL-60 cells were activated with 12-O-tetradecanoylphorbol-13-acetate (TPA) at 5 ng/mL for 4 hours. After washing both cells, 1 million HL-60 cells in 0.5 mL of RPMI 1640 were added to each well of the treated A549 cells under cell culture conditions. Following adhesion for 1 hour, nonattached HL-60 cells were washed away with 1x PBS for 3 times at room temperature. Cells were fixed with 1% Paraformaldehyde in 1x PBS for 10 min and washed 3 times with 1x PBS. Cells were photographed using a Leica Fluorescence Microscope (model, DMIRE2).


### 2.7. Statistical Analysis

Data were analyzed by using Mann-WhitneyU test (Kruskal-Wallis test for more than two groups). P
<
0.05 was considered statistically significant.


## 2.1. Cell Culture and Reagents

Human bronchial epithelial cells (BEAS-2B), human lung carcinoma cells (A549), and CF bronchi epithelial cells (IB3-1) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) medium (Invitrogen) supplemented with 10% fetal bovine serum (FBS). Recombinant human tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) were obtained from R&D Systems (Minneapolis, MN) and reconstituted in phosphate buffered saline (PBS) containing 0.1% bovine serum albumin (BSA). For cytokines stimulation, TNF-α and IL-1β were used at 10 ng/mL each.


## 2.2. Viral Transduction and shRNA-Mediated Gene Knocking Down

ESE-1 gene was knocked down in A549 cells using a shRNA helper-dependent adenoviral vector expressing two shRNAs from murine U6 gene promoter as described previously [36]. A C4HSU empty vector was used as control. For viral transduction, A549 cells were seeded at 2 × 105 cells per well in 6-well plates overnight, and then cells were transduced at 40–60% confluency with viral vector at 2500 particles/cell (or 50 moi) under serum-free conditions for two hours, followed by addition of media to a final concentration of 10% FBS. We usually achieved near 100% transduction at this vector concentration. Cells were then collected for protein extraction or RNA isolation at desired time points.


## 2.3. Transient Transfection and Luciferase Reporter Assay

For cell transfection and cotransfection experiments, BEAS-2B cells were seeded in 6-well plates and transfected at 50–60% confluency by using PolyFect transfection reagent (Qiagen) according to the manufacturer’s protocol. After 24 h, cells were harvested and luciferase activity was measured using a Dual Lucifcerase kit (Promega) and a luminometer (EG&G Berthold, BadWildbad, Germany) as described previously [37].


## 2.4. RNA Analysis

Cells were lysated and RNAs were extracted by using RNA spin Mini kit (GE Healthcare) according to manufacturer’s instructions. Total RNA (1μg) was reverse transcribed using random hexamers and SuperScript II reverse transcriptase (Invitrogen), and then the resulting template (20 ng cDNA) was used for each real-time PCR reaction (ABI Prism 7700, Applied Biosystems). Primers for humanESE-1 andICAM-1 were purchased from ACGT, Toronto. For relative quantification, PCR signals were compared between groups after normalization with GAPDH as an internal reference. Fold changes were calculated according to Livak and Schmittgen [38]. Primers used were hICAM1-F: GGAAGGTGTATGAACTGAGCAA, hICAM1-R: GGAGTCCAGTACACGGTGA, hese1-F: GGCGTCTTCAAGTTCCTGCG, hese1-R: CTCCCGTTTGTAGTAGTACCT, hGAPDH-F: GAAGGTGAAGGTCGGAGTC, hGAPDH-R: GAAGATGGTGATGGGATTTC.


## 2.5. Western Blotting Analysis

Cell lysates were run on an 8% SDS-polyacrylamide gel at 10μg each lane and transferred to nitrocellulose membrane (Bio-Rad) following electrophoresis. The membrane was then blocked with Tris-buffered saline with Tween-20 (TBST) containing 5% milk and probed with goat anti-humanICAM-1 antibody (R&D System, Minneapolis, USA) at 1 : 1000 dilution and rabbit anti-humanESE-1 antibody (R&D System, Minneapolis, USA) at 1 : 5000 dilution. Rabbit anti-human GAPDH antibodies (Trevigen, Gaithersburg, USA) were used at 1 : 3000 as a protein loading control. The horseradish peroxidase-conjugated secondary antibodies for rabbit anti-goat IgG were from Kirkegaard & Perry Laboratories and for goat anti-rabbit IgG were from Bio-Rad Laboratories. Detection of proteins was performed with enhanced chemiluminescence reagents (Amersham Pharmacia Biotech, Baie-d’Urfe, CA).


## 2.6. Cell Adhesion Assay

At day 0, 1 × 106 A549 cells were seeded on a 10 cm dish and virus vector particles were added at 100 moi (5000 particles per cell) for 1 hour in serum-free media. Fetal bovine serum was then added to final concentration of 10%. At day 5, the treated cells were harvested and seeded at 250,000 to each well of a-24-well plate. The reason for waiting for 5 days was to minimize the influence of inflammatory cytokines induced by the viral transduction. For visualization of cell-cell interactions, HL-60 cells were labeled with carboxyfluorescein succinimide ester (CFSE) at 5 μM in the cell culture for 5 min at 37°C and then washed 3 times with RPMI 1640. The labeled HL-60 cells were put back in the cell culture condition for 24 hours. The A549 cells were then stimulated with IL-1β and TNF-α each at 10 ng/mL for 6 hours and labeled HL-60 cells were activated with 12-O-tetradecanoylphorbol-13-acetate (TPA) at 5 ng/mL for 4 hours. After washing both cells, 1 million HL-60 cells in 0.5 mL of RPMI 1640 were added to each well of the treated A549 cells under cell culture conditions. Following adhesion for 1 hour, nonattached HL-60 cells were washed away with 1x PBS for 3 times at room temperature. Cells were fixed with 1% Paraformaldehyde in 1x PBS for 10 min and washed 3 times with 1x PBS. Cells were photographed using a Leica Fluorescence Microscope (model, DMIRE2).


## 2.7. Statistical Analysis

Data were analyzed by using Mann-WhitneyU test (Kruskal-Wallis test for more than two groups). P
<
0.05 was considered statistically significant.


## 3. Results

### 3.1.ESE-1 andICAM-1 Expression Are Upregulated in BEAS2B, IB3-1, and A549 Cells in Response to Inflammatory Cytokines

To examine whether bothESE-1 andICAM-1 are upregulated under proinflammatory conditions, BEAS-2B, IB3-1, and A549 cells were stimulated with a combination of proinflammatory cytokines TNF-α and IL-1β (10 ng/mL each) for 2 hours, and their mRNA levels were assessed by qRT-PCR. As shown in Figures 1(a) and 1(b), levels of mRNA for bothESE-1 andICAM-1 were increased significantly (except forESE-1 expression in A549 cells). We also observed a higher level ofESE-1 mRNA expression in A549 cells than BEAS-2B and IB3-1 cells. To further investigate at which time pointESE-1 andICAM-1 start responding to cytokine stimulation, we completed a time course study. As shown in Figures 1(c) and 1(d), theESE-1 mRNA expression peaked at 2 hours after stimulation in A549 cells whileICAM-1 mRNA expression reached a plateau at 4 hours after stimulation.Figure 1
Induction ofESE-1 andICAM-1 mRNA expression by TNF-α and IL-1β in different cell lines. (a, b)ESE-1 andICAM-1 mRNA expression in BEAS2B, IB3-1, and A549 cells after stimulation with TNF-α and IL-1β (10 ng/mL each) for 2 hours. The mRNA expression levels were determined by real-time quantitative RT-PCR and the fold of change was based on the mRNA level of non-induced cells. Data were normalized to GAPDH and values were shown in 2
-
Δ
Δ
CT as the mean ± SD, n
=
3, P
*
<
0.05. Statistics was performed as described in Materials and Methods. (c, d)ESE-1 andICAM-1 mRNA expression in A549 cells at different time points following cytokine stimulation. Cells were lysed at time points as indicated after TNF-α and IL-1β (10 ng/mL each) stimulation. The fold of change was based on the expression level at time 0 hour. Data were collected and analyzed as described in (a) and (b).
(a)
(b)
(c)
(d)


### 3.2.ICAM-1 Expression Is Downregulated by theESE-1 Knockdown in A549 Cells

Since bothESE-1 andICAM-1 mRNA expression were upregulated when stimulated by proinflammatory cytokines and given thatESE-1 is a transcription factor, we questioned whetherICAM-1 expression is transcriptionally regulated byESE-1. We used a helper-dependent adenovirus (HD-Ad) vector to knockdownESE-1 gene expression in A549 cells and examined the changes inICAM-1 expression. We selected A549 cells for the knockdown experiment, as these cells constitutively express relatively high levels ofESE-1, at both mRNA and protein levels. As expected, we found a significant reduction ofESE-1 mRNA following HD-Ad vector transduction (Figure 2(b)). Interestingly,ICAM-1 expression was also downregulated compared to the empty vector (C4HSU) control group (Figure 2(c)). We examinedESE-1 andICAM-1 expression at days 4 and 5 after transduction because viral vectors alone can induce the production of proinflammatory cytokines that can last for more than two days after viral transduction. We also used longer time points for cytokine stimulation in protein analysis (16 h) than in mRNA analysis (2 h), as changes in protein expression lag that of the mRNA.Figure 2
Effects ofESE-1 knockdown in A549 cells on the expression ofICAM-1. (a) Schematic diagrams of the helper-dependent adenovirus vectors (HD-Ad) that were used for theESE-1 knockdown experiments. ESE-1-RNAi expresses two shRNAs from the murine U6 gene promoter, and C4HSU is used as an empty vector control which does not express any transgene. ITR: inverted terminal repeat; Ψ: packing signal. (b, c)ESE-1 andICAM-1 mRNA expression in A549 cells 4 and 5 days after transduction with ESE-1-RNAi vector compared to that of C4HSU. Both groups were stimulated with TNF-α and IL-1β at 10 ng/mL each for 2 hours before cell lysis. The mRNA expression levels were normalized to GAPDH and the values were presented in 2
-
Δ
Δ
CT as the mean ± SD, n
=
3, P
*
<
0.05. Statistics was performed as described in Materials and Methods. (d) A representative western blot analysis ofICAM-1 expression with (+) and without (−) ESE-1-RNAi, compared to that of the C4HSU vector control group on day 4 and day 5 after transduction. SinceICAM-1 levels were very low, subgroup of cells were stimulated with TNF-α and IL-1β (10 ng/mL each) for 16 hours to visualize ICMA-1 protein expression before cell lysis.
(a)
(b)
(c)
(d)


### 3.3.ESE-1 Gene RegulatesICAM-1 Expression at the Transcriptional Level

To further investigate the regulatory mechanism, we developed anICAM-1 promoter reporter assay to examine the role ofESE-1 in the regulation ofICAM-1 expression. As shown in Figure 3(a), we made the reporter construct by inserting a 1.4 kb humanICAM-1 promoter in front of the luciferase reporter gene in pGL-3-basic vector (Promega). BEAS-2B cells were cotransfected with theICAM-1 promoter luciferase reporter plasmid and pcDNA3-ESE-1 or pcDNA3-empty as an empty vector control. BEAS-2B cells are noncancerous human bronchial epithelial cells which express minimal basal levels ofESE-1, allowing us to specifically overexpress the protein by transfection. Twenty-four hours after cotransfection, the luciferase activity was measured and as shown in Figure 3(b), the level of luciferase activity was increased in theESE-1 overexpression group compared to empty vector control group, suggesting thatESE-1 regulatesICAM-1 expression at the transcriptional level.Figure 3
Luciferase assay on BEAS2B cells after cotransfection with pcDNA3-ESE-1 and PGL3-ICAM-1 vector. (a) Schematic diagram of pGL3-ICAM-1 promoter reporter plasmid that contains anICAM-1 promoter sequence expressing the luciferase gene in the pGL3-Basic vector (Promega). (b) Luciferase activity assay. BEAS-2B cells were cotransfected with pGL3-ICAM-1 luciferase reporter plasmid and pcDNA3-ESE-1 or pcDNA3-empty vector [37]. The luciferase activity was measured 24 h after transfection.
(a)
(b)


### 3.4. Knockdown ofESE-1 Expression in A549 Cells Reduces Binding of HL-60 Cells

SinceICAM-1 is involved in cell-cell interactions, we performed a cell adhesion assay to investigate whether knockdown ofESE-1 gene could affect cellular adhesion. We found that, following transduction with ESE-1-RNAi viral vector, A549 cells had less binding of HL-60 cells compared with C4HSU empty vector transduced group (Figure 4).Figure 4
Cell adhesion assay of the effect ofESE-1 knocking down on HL-60 binding to A549 cells. (a) Images showing HL-60 cells attached to A549 cells at 40x magnification. (b) Quantification of cell binding. The average number of HL-60 cells per field was compared among groups withESE-1 knocking down or C4HSU empty vector transfection or nontransfected groups with or without cytokine stimulation (TNF-α and IL-1β at 10 ng/mL each). Cell numbers were counted under a microscope and data from six wells were presented as mean ± SD, P
*
<
0.05.
(a)
(b)


## 3.1.ESE-1 andICAM-1 Expression Are Upregulated in BEAS2B, IB3-1, and A549 Cells in Response to Inflammatory Cytokines

To examine whether bothESE-1 andICAM-1 are upregulated under proinflammatory conditions, BEAS-2B, IB3-1, and A549 cells were stimulated with a combination of proinflammatory cytokines TNF-α and IL-1β (10 ng/mL each) for 2 hours, and their mRNA levels were assessed by qRT-PCR. As shown in Figures 1(a) and 1(b), levels of mRNA for bothESE-1 andICAM-1 were increased significantly (except forESE-1 expression in A549 cells). We also observed a higher level ofESE-1 mRNA expression in A549 cells than BEAS-2B and IB3-1 cells. To further investigate at which time pointESE-1 andICAM-1 start responding to cytokine stimulation, we completed a time course study. As shown in Figures 1(c) and 1(d), theESE-1 mRNA expression peaked at 2 hours after stimulation in A549 cells whileICAM-1 mRNA expression reached a plateau at 4 hours after stimulation.Figure 1
Induction ofESE-1 andICAM-1 mRNA expression by TNF-α and IL-1β in different cell lines. (a, b)ESE-1 andICAM-1 mRNA expression in BEAS2B, IB3-1, and A549 cells after stimulation with TNF-α and IL-1β (10 ng/mL each) for 2 hours. The mRNA expression levels were determined by real-time quantitative RT-PCR and the fold of change was based on the mRNA level of non-induced cells. Data were normalized to GAPDH and values were shown in 2
-
Δ
Δ
CT as the mean ± SD, n
=
3, P
*
<
0.05. Statistics was performed as described in Materials and Methods. (c, d)ESE-1 andICAM-1 mRNA expression in A549 cells at different time points following cytokine stimulation. Cells were lysed at time points as indicated after TNF-α and IL-1β (10 ng/mL each) stimulation. The fold of change was based on the expression level at time 0 hour. Data were collected and analyzed as described in (a) and (b).
(a)
(b)
(c)
(d)


## 3.2.ICAM-1 Expression Is Downregulated by theESE-1 Knockdown in A549 Cells

Since bothESE-1 andICAM-1 mRNA expression were upregulated when stimulated by proinflammatory cytokines and given thatESE-1 is a transcription factor, we questioned whetherICAM-1 expression is transcriptionally regulated byESE-1. We used a helper-dependent adenovirus (HD-Ad) vector to knockdownESE-1 gene expression in A549 cells and examined the changes inICAM-1 expression. We selected A549 cells for the knockdown experiment, as these cells constitutively express relatively high levels ofESE-1, at both mRNA and protein levels. As expected, we found a significant reduction ofESE-1 mRNA following HD-Ad vector transduction (Figure 2(b)). Interestingly,ICAM-1 expression was also downregulated compared to the empty vector (C4HSU) control group (Figure 2(c)). We examinedESE-1 andICAM-1 expression at days 4 and 5 after transduction because viral vectors alone can induce the production of proinflammatory cytokines that can last for more than two days after viral transduction. We also used longer time points for cytokine stimulation in protein analysis (16 h) than in mRNA analysis (2 h), as changes in protein expression lag that of the mRNA.Figure 2
Effects ofESE-1 knockdown in A549 cells on the expression ofICAM-1. (a) Schematic diagrams of the helper-dependent adenovirus vectors (HD-Ad) that were used for theESE-1 knockdown experiments. ESE-1-RNAi expresses two shRNAs from the murine U6 gene promoter, and C4HSU is used as an empty vector control which does not express any transgene. ITR: inverted terminal repeat; Ψ: packing signal. (b, c)ESE-1 andICAM-1 mRNA expression in A549 cells 4 and 5 days after transduction with ESE-1-RNAi vector compared to that of C4HSU. Both groups were stimulated with TNF-α and IL-1β at 10 ng/mL each for 2 hours before cell lysis. The mRNA expression levels were normalized to GAPDH and the values were presented in 2
-
Δ
Δ
CT as the mean ± SD, n
=
3, P
*
<
0.05. Statistics was performed as described in Materials and Methods. (d) A representative western blot analysis ofICAM-1 expression with (+) and without (−) ESE-1-RNAi, compared to that of the C4HSU vector control group on day 4 and day 5 after transduction. SinceICAM-1 levels were very low, subgroup of cells were stimulated with TNF-α and IL-1β (10 ng/mL each) for 16 hours to visualize ICMA-1 protein expression before cell lysis.
(a)
(b)
(c)
(d)


## 3.3.ESE-1 Gene RegulatesICAM-1 Expression at the Transcriptional Level

To further investigate the regulatory mechanism, we developed anICAM-1 promoter reporter assay to examine the role ofESE-1 in the regulation ofICAM-1 expression. As shown in Figure 3(a), we made the reporter construct by inserting a 1.4 kb humanICAM-1 promoter in front of the luciferase reporter gene in pGL-3-basic vector (Promega). BEAS-2B cells were cotransfected with theICAM-1 promoter luciferase reporter plasmid and pcDNA3-ESE-1 or pcDNA3-empty as an empty vector control. BEAS-2B cells are noncancerous human bronchial epithelial cells which express minimal basal levels ofESE-1, allowing us to specifically overexpress the protein by transfection. Twenty-four hours after cotransfection, the luciferase activity was measured and as shown in Figure 3(b), the level of luciferase activity was increased in theESE-1 overexpression group compared to empty vector control group, suggesting thatESE-1 regulatesICAM-1 expression at the transcriptional level.Figure 3
Luciferase assay on BEAS2B cells after cotransfection with pcDNA3-ESE-1 and PGL3-ICAM-1 vector. (a) Schematic diagram of pGL3-ICAM-1 promoter reporter plasmid that contains anICAM-1 promoter sequence expressing the luciferase gene in the pGL3-Basic vector (Promega). (b) Luciferase activity assay. BEAS-2B cells were cotransfected with pGL3-ICAM-1 luciferase reporter plasmid and pcDNA3-ESE-1 or pcDNA3-empty vector [37]. The luciferase activity was measured 24 h after transfection.
(a)
(b)


## 3.4. Knockdown ofESE-1 Expression in A549 Cells Reduces Binding of HL-60 Cells

SinceICAM-1 is involved in cell-cell interactions, we performed a cell adhesion assay to investigate whether knockdown ofESE-1 gene could affect cellular adhesion. We found that, following transduction with ESE-1-RNAi viral vector, A549 cells had less binding of HL-60 cells compared with C4HSU empty vector transduced group (Figure 4).Figure 4
Cell adhesion assay of the effect ofESE-1 knocking down on HL-60 binding to A549 cells. (a) Images showing HL-60 cells attached to A549 cells at 40x magnification. (b) Quantification of cell binding. The average number of HL-60 cells per field was compared among groups withESE-1 knocking down or C4HSU empty vector transfection or nontransfected groups with or without cytokine stimulation (TNF-α and IL-1β at 10 ng/mL each). Cell numbers were counted under a microscope and data from six wells were presented as mean ± SD, P
*
<
0.05.
(a)
(b)


## 4. Discussion

In response to environmental perturbations, airway epithelia produce and release a variety of inflammatory cytokines, including TNF-α, IL-1, IL-6, and IL-8 [39]. A number of studies have shown that the epithelium of patients who have airway inflammatory diseases is structurally and functionally altered, and that bronchial epithelial cells that are isolated from patients with asthma or cystic fibrosis (CF) express increased levels of cytokines (IL-8, IL-25) [40, 41]. It is thus important to fully understand the gene regulation in airway epithelium in order to alleviate airway inflammatory diseases.In this study, we show that bothESE-1 andICAM-1 mRNAs are upregulated in lung epithelial cell lines after treatment with TNF-α and IL-1β. However, while both genes can be activated by the NF-κB [42], we questioned whether there was any regulatory relationship between the two. The regulation ofICAM-1 gene expression is primarily at the transcriptional level by several signaling pathways including protein kinase C (PKC), mitogen activated protein (MAP) kinase (JNK, ERK, and P38), and NF-κB. The humanICAM-1 gene promoter contains binding sites for many transcription factors, including AP-1, C/EBP, Ets, NF-κB, STAT, and Sp1 [42]. SinceESE-1 is a member of Ets family transcription factors which shares DNA binding sites, we decided to examine whetherESE-1 regulatedICAM-1 expression. To investigate the relationship between these two genes, we used helper-dependent adenovirus vector containing shRNA-ESE-1 to knockdownESE-1. We observed thatICAM-1 expression in A549 cells was indeed decreased after knocking downESE-1.To further investigate on the mechanism of this relationship betweenESE-1 andICAM-1, we usedICAM-1 promoter luciferase reporter assays to determine whether this upregulation was at the transcription level. Upon cotransfecting the luciferase reporter plasmid with anESE-1 gene expressing plasmid into BEAS-2B cells, we observed an increase in luciferase activity whenESE-1 gene was overexpressed compared to the cells cotransfected with an empty vector plasmid. This result suggests thatESE-1 regulatesICAM-1 expression at the transcriptional level. However, we noted that the level of upregulation was not dramatic, but under chronic inflammatory conditions, a small change inICAM-1 levels may have a significant impact on disease progression.We also performed a cell adhesion assay to investigate whether knocking downESE-1 could affect cell adhesion of epithelial cells. We show that, following transduction with ESE-1-RNAi vector, A549 cells exhibited less binding of HL-60 cells compared to groups of transduced with C4HSU empty vector or untransduced. This suggests that knocking downESE-1 in A549 cells causes downregulation ofICAM-1 gene expression which in turn results in a reduced binding interaction between HL-60 and A549 cells.ICAM-1 is a key molecule that contributes to the control of inflammatory process. Although our finding ofESE-1 regulatingICAM-1 expression in this work is interesting, there are limitations to its clinical applications. First,ESE-1 knocking down was carried out only in A549 cells, not in primary CF lung epithelial cells. Second, similar experiments have not been performed in any CF animal model. Future studies on this regulation in other model systems will be important for the development of effective anti-inflammatory strategies suitable for therapeutic intervention of inflammatory conditions such as CF. SinceESE-1 is an transcription factor, it could be a potential drug target for screening small molecules that inhibit its expression.



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*Source: 547928-2015-06-21.xml*