# Genetic Deletion and Pharmacological Inhibition of PI3Kγ Reduces Neutrophilic Airway Inflammation and Lung Damage in Mice with Cystic Fibrosis-Like Lung Disease

Authors: Maria Galluzzo; Elisa Ciraolo; Monica Lucattelli; Eriola Hoxha; Martina Ulrich; Carlo Cosimo Campa; Giuseppe Lungarella; Gerd Doring; Zhe Zhou-Suckow; Marcus Mall; Emilio Hirsch; Virginia De Rose
Journal: Mediators of Inflammation
 (2015)

---

## Abstract

Purpose. Neutrophil-dominated airway inflammation is a key feature of progressive lung damage in cystic fibrosis (CF). Thus, reducing airway inflammation is a major goal to prevent lung damage in CF. However, current anti-inflammatory drugs have shown several limits. PI3Kγ plays a pivotal role in leukocyte recruitment and activation; in the present study we determined the effects of genetic deletion and pharmacologic inhibition of PI3Kγ on airway inflammation and structural lung damage in a mouse model of CF lung disease. Methods. βENaC overexpressing mice (βENaC-Tg) were backcrossed with PI3Kγ-deficient (PI
3
K
γ
KO) mice. Tissue damage was assessed by histology and morphometry and inflammatory cell number was evaluated in bronchoalveolar lavage fluid (BALF).  Furthermore, we assessed the effect of a specific PI3Kγ inhibitor  (AS-605240) on  inflammatory cell number in BALF. Results. Genetic deletion of PI3Kγ decreased neutrophil numbers in BALF of PI
3
K
γ
KO/βENaC-Tg mice, and this was associated with reduced emphysematous changes. Treatment with the PI3Kγ inhibitor AS-605240 decreased the number of neutrophils in BALF of βENaC-Tg mice, reproducing the effect observed with genetic deletion of the enzyme. Conclusions. These results demonstrate the biological efficacy of both genetic deletion and pharmacological inhibition of PI3Kγ in reducing chronic neutrophilic inflammation in CF-like lung disease in vivo.


---

## Body

## 1. Introduction

Cystic fibrosis (CF), the most common genetic disease in Caucasian populations, results from mutations in a single gene encoding for 1480 residues transmembrane glycoprotein, the cystic fibrosis transmembrane conductance regulator (CFTR), that regulates cAMP-mediated chloride conductance at the apical surface of secretory epithelia [1, 2]. Impaired CFTR-mediated secretion of Cl- and bicarbonate results in dehydration and acidification of the airway surface liquid, which in turn causes impaired mucociliary clearance and bacterial killing. These defects trigger a progressive lung disease characterized by airway mucus obstruction, chronic neutrophilic inflammation, bacterial infection, and structural lung damage that remains the major cause of morbidity and mortality in patients with CF [3].A growing number ofin vitro andin vivo studies support the notion that chronic neutrophilic inflammation with the release of damaging neutrophil products, such as neutrophil elastase, constitutes a key risk factor in early structural lung damage and lung function decline in CF [4–6]. Neutrophilic airway inflammation is augmented after onset of chronic bacterial infection withPseudomonas aeruginosa and other pathogens. In this context, the inflammatory response in the CF lung is nonresolving and self-perpetuating, and a vicious cycle of neutrophilic inflammation, noxious mediator release, and overwhelmed defenses amplifies inflammation, perpetuates infection and contributes to irreversible lung damage and disease progression [7–9]. Therefore, anti-inflammatory therapy, combined with antibiotic therapy, appears crucial to prevent chronic lung damage. However, traditional therapeutic strategies, as well as more recently studied anti-inflammatory drugs, have shown several limitations and limited clinical benefit [8–10]. Clearly, novel approaches have to be undertaken to provide effective anti-inflammatory therapy to CF patients. One possibility is to interfere with leukocyte trafficking into CF airways. Trafficking of leukocytes is controlled by chemotactic factors which bind to heterotrimeric G-protein-coupled receptors (GPCR) and trigger a complex set of signaling pathways inside the cell involving the generation of second messengers like phosphoinositides. Phosphoinositides are substrates of the phosphoinositide 3-kinases (PI3Ks), enzymes that catalyze the phosphorylation of the phosphatidylinositol at the 3rd position of the inositol ring. PI3Ks modulate a wide number of cellular functions such as proliferation and survival, cytoskeletal remodeling, and membrane trafficking and represent important mediators in the signaling cascade leading to the initiation of the inflammatory response [11–14]. PI3Ks can be divided in three classes (I, II, and III) based on their biochemical properties. Leukocytes express all four known isoforms of class I PI3Ks, namely, PI3K α, β, δ, and γ [14]; nonetheless PI3Kγ plays a fundamental role in leukocyte migration and function by acting as a chemokine sensor and regulating neutrophil oxidative burst, T cell proliferation, and mast degranulation. We therefore hypothesized that PI3Kγ plays a pivotal role in mediating leukocyte recruitment and activation and may thus represent a potential target for anti-inflammatory treatment to reduce neutrophilic airway inflammation and lung damage in CF. To test this hypothesis, we used transgenic mice with airway-specific overexpression of the epithelial Na+ channel (ENaC) and determined the effects of genetic deletion and pharmacologic inhibition of PI3Kγ [15–17].


## 2. Materials and Methods

### 2.1. Mice

PI
3
K
γ
WT/βENaC-Tg (βENaC-Tg) [15–18] and PI3Kγ-deficient (PI
3
K
γ
KO, Harlan, Italy) mice on the C57BL/6 background were intercrossed to generate βENaC-Tg/PI
3
K
γ
KO mice. All experiments were performed in 7- to 8-week-old adult mice. βENaC-Tg, PI
3
K
γ
KO, PI
3
K
γ
KO/βENaC-Tg, and wild-type (PI
3
K
γ
WT) mice were housed in a pathogen-free animal facility at the Istituto per la Ricerca e la Cura del Cancro, University of Turin, in accordance with the Institutional Animal Welfare Guidelines and Italian legislation. The animal study protocols were reviewed and approved by the Institutional Animal Ethics Committee of the Istituto per la Ricerca e la Cura del Cancro, University of Turin, Turin, Italy, and performed according to the Institutional Animal Welfare Guidelines and Italian legislation.


### 2.2. Assessment of Inflammatory Cells in Bronchoalveolar Lavage

Inflammatory cell numbers were assessed in the broncoalveolar fluid (BALF) ofPI
3
K
γ
WT mice and of PI
3
K
γ
WT/βENaC-Tg, PI
3
K
γ
KO, and PI
3
K
γ
KO/βENaC-Tg mice. Briefly, mice from each genotype were sacrificed and BALF was then collected by lavaging lungs in situ with 3 × 1-mL volumes of PBS. After centrifugation of the BALFs, cell pellets, in 500 μL of RPMI medium, were deposited onto glass slides using a Cytospin Cytocentrifuge. Slides were then stained using the Diff-Quick system (MICROPTIC S.L., Spain) and a differential cell count was performed as previously described [19]. In addition, BALF inflammatory cells were also analyzed in mice treated with the PI3Kγ inhibitor AS-605240 [5-(quinoxalin-6-ylmethylidene)-1,3-thiazolidine-2,4-dione] (Sigma, Germany). PI
3
K
γ
WT and PI
3
K
γ
WT/βENaC-Tg mice were treated once daily for 3 days with the AS-605240 by intraperitoneal injection of 10 mg/kg of the drug or vehicle (0.5% carboxymethyl cellulose, 0.25% Tween) alone.


### 2.3. Lung Histology and Morphometry

Animals of each group were sacrificed under anaesthesia with pentobarbital (60 mg/Kg) and the lungs fixed intratracheally with buffered formalin (5%) at a constant pressure of 20 cm H2O. Lung volume (V) was measured by water displacement according to Scherle [20]. Sagittal sections of each pair of lungs were cut and stained with haematoxylin/eosin. The slides were coded to prevent bias. Morphometric evaluations included determination of the average interalveolar distance (mean linear intercept: Lm) [21] and internal surface area (ISA) estimated by the Lm method at postfixation lung volume by the formula 4V/Lm, where V is the postfixation lung volume [22]. For the determination of the Lm for each pair of lungs, 40 histological fields were evaluated both vertically and horizontally. The development of goblet cell metaplasia was evaluated by periodic acid-Schiff reaction (PAS) according to standard histological protocols [23]. The total number of cells, as well as the percentage of PAS-positive cells, was determined. The number of cells in airways that demonstrated PAS staining was determined by examining eight intrapulmonary airways per section and counting at least 3,000 cells/section. Data were reported as the percentage of positive cells per total cells.


### 2.4. Statistical Analysis

Statistical analyses were performed using one-way analysis of variance. Survival curves were compared using Kaplan-Meier log rank analysis.P
<
0.05 was considered statistically significant and “n” represents the number of mice in each experimental group. Data are expressed as mean ± SD.


## 2.1. Mice

PI
3
K
γ
WT/βENaC-Tg (βENaC-Tg) [15–18] and PI3Kγ-deficient (PI
3
K
γ
KO, Harlan, Italy) mice on the C57BL/6 background were intercrossed to generate βENaC-Tg/PI
3
K
γ
KO mice. All experiments were performed in 7- to 8-week-old adult mice. βENaC-Tg, PI
3
K
γ
KO, PI
3
K
γ
KO/βENaC-Tg, and wild-type (PI
3
K
γ
WT) mice were housed in a pathogen-free animal facility at the Istituto per la Ricerca e la Cura del Cancro, University of Turin, in accordance with the Institutional Animal Welfare Guidelines and Italian legislation. The animal study protocols were reviewed and approved by the Institutional Animal Ethics Committee of the Istituto per la Ricerca e la Cura del Cancro, University of Turin, Turin, Italy, and performed according to the Institutional Animal Welfare Guidelines and Italian legislation.


## 2.2. Assessment of Inflammatory Cells in Bronchoalveolar Lavage

Inflammatory cell numbers were assessed in the broncoalveolar fluid (BALF) ofPI
3
K
γ
WT mice and of PI
3
K
γ
WT/βENaC-Tg, PI
3
K
γ
KO, and PI
3
K
γ
KO/βENaC-Tg mice. Briefly, mice from each genotype were sacrificed and BALF was then collected by lavaging lungs in situ with 3 × 1-mL volumes of PBS. After centrifugation of the BALFs, cell pellets, in 500 μL of RPMI medium, were deposited onto glass slides using a Cytospin Cytocentrifuge. Slides were then stained using the Diff-Quick system (MICROPTIC S.L., Spain) and a differential cell count was performed as previously described [19]. In addition, BALF inflammatory cells were also analyzed in mice treated with the PI3Kγ inhibitor AS-605240 [5-(quinoxalin-6-ylmethylidene)-1,3-thiazolidine-2,4-dione] (Sigma, Germany). PI
3
K
γ
WT and PI
3
K
γ
WT/βENaC-Tg mice were treated once daily for 3 days with the AS-605240 by intraperitoneal injection of 10 mg/kg of the drug or vehicle (0.5% carboxymethyl cellulose, 0.25% Tween) alone.


## 2.3. Lung Histology and Morphometry

Animals of each group were sacrificed under anaesthesia with pentobarbital (60 mg/Kg) and the lungs fixed intratracheally with buffered formalin (5%) at a constant pressure of 20 cm H2O. Lung volume (V) was measured by water displacement according to Scherle [20]. Sagittal sections of each pair of lungs were cut and stained with haematoxylin/eosin. The slides were coded to prevent bias. Morphometric evaluations included determination of the average interalveolar distance (mean linear intercept: Lm) [21] and internal surface area (ISA) estimated by the Lm method at postfixation lung volume by the formula 4V/Lm, where V is the postfixation lung volume [22]. For the determination of the Lm for each pair of lungs, 40 histological fields were evaluated both vertically and horizontally. The development of goblet cell metaplasia was evaluated by periodic acid-Schiff reaction (PAS) according to standard histological protocols [23]. The total number of cells, as well as the percentage of PAS-positive cells, was determined. The number of cells in airways that demonstrated PAS staining was determined by examining eight intrapulmonary airways per section and counting at least 3,000 cells/section. Data were reported as the percentage of positive cells per total cells.


## 2.4. Statistical Analysis

Statistical analyses were performed using one-way analysis of variance. Survival curves were compared using Kaplan-Meier log rank analysis.P
<
0.05 was considered statistically significant and “n” represents the number of mice in each experimental group. Data are expressed as mean ± SD.


## 3. Results

### 3.1. Genetic Deletion of PI3Kγ Reduces Neutrophilic Airway Inflammation and Mortality in βENaC-Tg Mice

As observed in previous studies,βENaC-Tg (PI
3
K
γ
WT/βENaC-Tg; Figure 1(a)) mice on the C56BL/6J background exhibited a spontaneous mortality of ~23% [18, 24]. Deletion of PI3Kγ had no effect on survival in wild-type mice; however, in the presence of the βENaC transgene (PI
3
K
γ
KO/βENaC-Tg), PI3Kγ loss significantly reduced the mortality by ~50%, since at 60 days the survival rate is more than 85% (P
<
0.05, Figure 1(a)).Figure 1
Effect of genetic deletion of PI3Kγ on mortality and airway inflammation in βENaC-Tg mice. (a) Survival curves for the different groups of mice studied (P
<
0.05). (b) Neutrophil numbers were assessed in BALF of PI
3
K
γ
WT, PI
3
K
γ
WT/βENaC-Tg, PI
3
K
γ
KO, and PI
3
K
γ
KO/βENaC-Tg mice. Neutrophils are expressed as cell numbers per mL of BALF (n
=
10 mice for each group). Comparison between the different groups was performed by one-way analysis of variance. P
*
*
*
<
0.001
PI
3
K
γ
WT
versus
PI
3
K
γ
WT/βENaC-Tg and P
*
*
*
<
0.001
PI
3
K
γ
WT/βENaC-Tgversus
PI
3
K
γ
KO/βENaC-Tg. (c) Immunofluorescent detection of neutrophils in lung tissues of PI
3
K
γ
WT/βENaC-Tg, PI
3
K
γ
KO, and PI
3
K
γ
KO/βENaC-Tg mice. Neutrophils were stained by using monoclonal rat antibodies to neutrophils (clone 7/4, Acris) and nuclei with DAPI. (d) Lymphocyte numbers were assessed in BALF of PI
3
K
γ
WT, PI
3
K
γ
WT/βENaC-Tg, PI
3
K
γ
KO, and PI
3
K
γ
KO/βENaC-Tg mice. Lymphocytes are expressed as number of cells per mL of BALF (n
=
10 mice for each group) P
*
<
0.05
PI
3
K
γ
WT
versus
PI
3
K
γ
WT/βENaC-Tg and P
*
*
<
0.01
PI
3
K
γ
KO
versus
PI
3
K
γ
KO/βENaC-Tg. (e) Macrophage numbers were assessed in BALF of PI
3
K
γ
WT, PI
3
K
γ
WT/βENaC-Tg, PI
3
K
γ
KO, and PI
3
K
γ
KO/βENaC-Tg mice. Macrophages are expressed as number of cells per mL of BALF (n
=
10 mice for each group). P
*
<
0.05
PI
3
K
γ
WT
versus
PI
3
K
γ
WT/βENaC-Tg and P
*
*
<
0.01
PI
3
K
γ
KO
versus
PI
3
K
γ
KO/βENaC-Tg.
(a)
(b)
(c)
(d)
(e)To determine the effect of genetic deletion of PI3Kγ on airway inflammation, we compared inflammatory cell numbers in BAL fluid from surviving PI
3
K
γ
WT/βENaC-Tg and PI
3
K
γ
KO/βENaC-Tg mice. As expected, in homozygous PI
3
K
γ
WT and PI
3
K
γ
KO control mice, neutrophils were rarely detected in the BALF (Figure 1(b)) as well as in the airways lumen (Figure 1(c)). The number of neutrophils, in BALF and in the airways lumen, was markedly elevated in PI
3
K
γ
WT/βENaC-Tg mice (Figures 1(b) and 1(c)). On the contrary, the absence of PI3Kγ expression in PI
3
K
γ
KO/βENaC-Tg mice led to a large reduction of neutrophil recruitment into the lung if compared to PI
3
K
γ
WT/βENaC-Tg mice (Figure 1(b)). Nonetheless, deletion of PI3Kγ did not affect macrophage and lymphocyte recruitment as no differences were detected between PI
3
K
γ
KO/βENaC-Tg and PI
3
K
γ
WT/βENaC-Tg mice in BALF (Figures 1(d) and 1(e)).


### 3.2. Genetic Deletion of PI3Kγ Reduces Structural Lung Damage in βENaC-Tg Mice

Chronic inflammation, inPI
3
K
γ
WT/βENaC-Tg mice, triggers emphysema with distal airspace enlargement and alveolar destruction resulting in reduced lung tissue density and increased lung compliance [6, 17, 19]. To assess the protective effects of the genetic deletion of PI3Kγ on emphysema-like changes in PI
3
K
γ
KO/βENaC-Tg mice, we determined the averaged interalveolar distance (mean linear intercept, Lm) and the internal surface area (ISA) estimated by the Lm method at postfixation lung volume. ISA and Lm were not altered in the lungs of controls PI
3
K
γ
WT and PI
3
K
γ
KO mice (Figures 2(a) and 2(b)), and morphological analysis showed a well-fixed normal parenchyma with normal airways (data not shown). As expected from previous studies [6, 17, 19], PI
3
K
γ
WT/βENaC-Tg mice lungs showed significant emphysematous changes (Figures 2(a)–2(c)) while the genetic deletion of PI3Kγ in PI
3
K
γ
KO/βENaC-Tg mice resulted in a significant reduction of the degree of emphysema, as assessed by both morphometric analyses (ISA: P
<
0.0002 versus PI
3
K
γ
WT/βENaC-Tg mice; Lm: P
<
0.0003
versus
PI
3
K
γ
WT/βENaC-Tg mice; Figures 2(a) and 2(b)) and morphology (Figure 2(c)).Figure 2
Genetic deletion of PI3Kγ decreases emphysema in βENaC-Tg mice. Mouse lungs were fixed in 4% formalin and embedded in paraffin and 5 µm sections were stained with hematoxylin/eosin; assessment of emphysema included the internal surface area (ISA) at postfixation lung volume and the morphometric assessment of the average inter-alveolar distance (mean linear intercept: Lm). (a) ISA and (b) Lm from 8-week-old wild type (PI
3
K
γ
WT), βENaC-Tg, PI
3
K
γ
KO, and PI
3
K
γ
KO/βENaC-Tg mice (n = 8–10 mice for each group). Comparison among groups was performed using one-way analysis of variance. (a) P
*
<
0.05
PI
3
K
γ
WT
versus
PI
3
K
γ
WT/βENaC-Tg, P
*
*
<
0.01
PI
3
K
γ
KO
versus
PI
3
K
γ
WT/βENaC-Tg, P
*
*
*
<
0.001
PI
3
K
γ
WT/βENaC-Tgversus
PI
3
K
γ
KO/βENaC-Tg; (b) P
*
*
<
0.01
PI
3
K
γ
WT
versus
PI
3
K
γ
WT/βENaC-Tg, and P
<
0.01
PI
3
K
γ
KO
versus
PI
3
K
γ
WT/βENaC-Tg, P
*
*
*
<
0.001
PI
3
K
γ
WT/βENaC-Tgversus
PI
3
K
γ
KO/βENaC-Tg mice. (c) Representative histological sections from the lung of 8-week-old βENaC-Tg mousee (left) showing evident areas of emphysema and PI
3
K
γ
KO/βENaC-Tg (right) mouse showing a focal areas of mild emphysema. Haematoxylin and eosin stain. Original magnification ×40.
(a)
(b)
(c)In addition to neutrophilic inflammation, goblet cell metaplasia and mucus obstruction were a common feature of the airways of adultPI
3
K
γ
WT/βENaC-Tg mice [19]. Since neutrophil products, such as neutrophil elastase, have been implicated in goblet cell metaplasia and mucin hypersecretion in CF [25, 26], we assessed the effects of genetic deletion of PI3Kγ on goblet cell metaplasia. Goblet cells were not observed in PI
3
K
γ
WT and PI
3
K
γ
KO mice; in PI
3
K
γ
KO/βENaC-Tg mice, the goblet cell metaplasia appeared reduced compared to PI
3
K
γ
WT/βENaC-Tg mice; however, this difference was not statistically significant, based on the variability and the number of mice included in our studies (data not shown).


### 3.3. Pharmacological Inhibition of PI3Kγ Reduces Neutrophilic Airway Inflammation in βENaC-Tg Mice

Next we tested effects of pharmacological inhibition of PI3Kγ by using the inhibitor AS-605240 on airway inflammation in βENaC-Tg mice. Treatment of βENaC-Tg mice with AS-605240 but not with vehicle alone reduced neutrophil infiltrates in BALF of βENaC-Tg mice (Figure 3(a)). In contrast, as observed in PI
3
K
γ
KO/βENaC-Tg mice, the PI3Kγ inhibitor had no effect on the recruitment of macrophages or lymphocytes into the lung (Figures 3(b) and 3(c)).Figure 3
Pharmacological inhibition of PI3Kγ decreases neutrophilic airway inflammation in βENaC-Tg mice. Neutrophils (a), lymphocytes (b), and macrophages (c) numbers were determined in BAL fluid of control (WT/Vehicle) and βENaC-Tg mice untreated or treated with the PI3Kγ inhibitor AS-605240 (βENaC-Tg/AS-605240) or with vehicle (βENaC-Tg/Vehicle). Cells are expressed as number per mL of BAL fluid. (a) P
*
*
<
0.01 WT/Vehicleversus βENaC-Tg/Vehicle, P
*
*
<
0.01
βENaC-Tg/Vehicleversus βENaC-Tg/AS-605240 and P
*
<
0.05 WT/Vehicleversus
PI
3
K
γ
WT/βENaC-Tg. (b) P
*
*
<
0.01 WT/Vehicleversus βENaC-Tg/Vehicle and P
*
<
0.05 WT/Vehicleversus
PI
3
K
γ
WT/βENaC-Tg. (c). P
*
*
<
0.01 WT/Vehicleversus βENaC-Tg/Vehicle, P
*
*
<
0.01 WT/Vehicleversus βENaC-Tg/AS-605240, and P
*
<
0.05 WT/Vehicleversus
PI
3
K
γ
WT/βENaC-Tg.
(a)
(b)
(c)


## 3.1. Genetic Deletion of PI3Kγ Reduces Neutrophilic Airway Inflammation and Mortality in βENaC-Tg Mice

As observed in previous studies,βENaC-Tg (PI
3
K
γ
WT/βENaC-Tg; Figure 1(a)) mice on the C56BL/6J background exhibited a spontaneous mortality of ~23% [18, 24]. Deletion of PI3Kγ had no effect on survival in wild-type mice; however, in the presence of the βENaC transgene (PI
3
K
γ
KO/βENaC-Tg), PI3Kγ loss significantly reduced the mortality by ~50%, since at 60 days the survival rate is more than 85% (P
<
0.05, Figure 1(a)).Figure 1
Effect of genetic deletion of PI3Kγ on mortality and airway inflammation in βENaC-Tg mice. (a) Survival curves for the different groups of mice studied (P
<
0.05). (b) Neutrophil numbers were assessed in BALF of PI
3
K
γ
WT, PI
3
K
γ
WT/βENaC-Tg, PI
3
K
γ
KO, and PI
3
K
γ
KO/βENaC-Tg mice. Neutrophils are expressed as cell numbers per mL of BALF (n
=
10 mice for each group). Comparison between the different groups was performed by one-way analysis of variance. P
*
*
*
<
0.001
PI
3
K
γ
WT
versus
PI
3
K
γ
WT/βENaC-Tg and P
*
*
*
<
0.001
PI
3
K
γ
WT/βENaC-Tgversus
PI
3
K
γ
KO/βENaC-Tg. (c) Immunofluorescent detection of neutrophils in lung tissues of PI
3
K
γ
WT/βENaC-Tg, PI
3
K
γ
KO, and PI
3
K
γ
KO/βENaC-Tg mice. Neutrophils were stained by using monoclonal rat antibodies to neutrophils (clone 7/4, Acris) and nuclei with DAPI. (d) Lymphocyte numbers were assessed in BALF of PI
3
K
γ
WT, PI
3
K
γ
WT/βENaC-Tg, PI
3
K
γ
KO, and PI
3
K
γ
KO/βENaC-Tg mice. Lymphocytes are expressed as number of cells per mL of BALF (n
=
10 mice for each group) P
*
<
0.05
PI
3
K
γ
WT
versus
PI
3
K
γ
WT/βENaC-Tg and P
*
*
<
0.01
PI
3
K
γ
KO
versus
PI
3
K
γ
KO/βENaC-Tg. (e) Macrophage numbers were assessed in BALF of PI
3
K
γ
WT, PI
3
K
γ
WT/βENaC-Tg, PI
3
K
γ
KO, and PI
3
K
γ
KO/βENaC-Tg mice. Macrophages are expressed as number of cells per mL of BALF (n
=
10 mice for each group). P
*
<
0.05
PI
3
K
γ
WT
versus
PI
3
K
γ
WT/βENaC-Tg and P
*
*
<
0.01
PI
3
K
γ
KO
versus
PI
3
K
γ
KO/βENaC-Tg.
(a)
(b)
(c)
(d)
(e)To determine the effect of genetic deletion of PI3Kγ on airway inflammation, we compared inflammatory cell numbers in BAL fluid from surviving PI
3
K
γ
WT/βENaC-Tg and PI
3
K
γ
KO/βENaC-Tg mice. As expected, in homozygous PI
3
K
γ
WT and PI
3
K
γ
KO control mice, neutrophils were rarely detected in the BALF (Figure 1(b)) as well as in the airways lumen (Figure 1(c)). The number of neutrophils, in BALF and in the airways lumen, was markedly elevated in PI
3
K
γ
WT/βENaC-Tg mice (Figures 1(b) and 1(c)). On the contrary, the absence of PI3Kγ expression in PI
3
K
γ
KO/βENaC-Tg mice led to a large reduction of neutrophil recruitment into the lung if compared to PI
3
K
γ
WT/βENaC-Tg mice (Figure 1(b)). Nonetheless, deletion of PI3Kγ did not affect macrophage and lymphocyte recruitment as no differences were detected between PI
3
K
γ
KO/βENaC-Tg and PI
3
K
γ
WT/βENaC-Tg mice in BALF (Figures 1(d) and 1(e)).


## 3.2. Genetic Deletion of PI3Kγ Reduces Structural Lung Damage in βENaC-Tg Mice

Chronic inflammation, inPI
3
K
γ
WT/βENaC-Tg mice, triggers emphysema with distal airspace enlargement and alveolar destruction resulting in reduced lung tissue density and increased lung compliance [6, 17, 19]. To assess the protective effects of the genetic deletion of PI3Kγ on emphysema-like changes in PI
3
K
γ
KO/βENaC-Tg mice, we determined the averaged interalveolar distance (mean linear intercept, Lm) and the internal surface area (ISA) estimated by the Lm method at postfixation lung volume. ISA and Lm were not altered in the lungs of controls PI
3
K
γ
WT and PI
3
K
γ
KO mice (Figures 2(a) and 2(b)), and morphological analysis showed a well-fixed normal parenchyma with normal airways (data not shown). As expected from previous studies [6, 17, 19], PI
3
K
γ
WT/βENaC-Tg mice lungs showed significant emphysematous changes (Figures 2(a)–2(c)) while the genetic deletion of PI3Kγ in PI
3
K
γ
KO/βENaC-Tg mice resulted in a significant reduction of the degree of emphysema, as assessed by both morphometric analyses (ISA: P
<
0.0002 versus PI
3
K
γ
WT/βENaC-Tg mice; Lm: P
<
0.0003
versus
PI
3
K
γ
WT/βENaC-Tg mice; Figures 2(a) and 2(b)) and morphology (Figure 2(c)).Figure 2
Genetic deletion of PI3Kγ decreases emphysema in βENaC-Tg mice. Mouse lungs were fixed in 4% formalin and embedded in paraffin and 5 µm sections were stained with hematoxylin/eosin; assessment of emphysema included the internal surface area (ISA) at postfixation lung volume and the morphometric assessment of the average inter-alveolar distance (mean linear intercept: Lm). (a) ISA and (b) Lm from 8-week-old wild type (PI
3
K
γ
WT), βENaC-Tg, PI
3
K
γ
KO, and PI
3
K
γ
KO/βENaC-Tg mice (n = 8–10 mice for each group). Comparison among groups was performed using one-way analysis of variance. (a) P
*
<
0.05
PI
3
K
γ
WT
versus
PI
3
K
γ
WT/βENaC-Tg, P
*
*
<
0.01
PI
3
K
γ
KO
versus
PI
3
K
γ
WT/βENaC-Tg, P
*
*
*
<
0.001
PI
3
K
γ
WT/βENaC-Tgversus
PI
3
K
γ
KO/βENaC-Tg; (b) P
*
*
<
0.01
PI
3
K
γ
WT
versus
PI
3
K
γ
WT/βENaC-Tg, and P
<
0.01
PI
3
K
γ
KO
versus
PI
3
K
γ
WT/βENaC-Tg, P
*
*
*
<
0.001
PI
3
K
γ
WT/βENaC-Tgversus
PI
3
K
γ
KO/βENaC-Tg mice. (c) Representative histological sections from the lung of 8-week-old βENaC-Tg mousee (left) showing evident areas of emphysema and PI
3
K
γ
KO/βENaC-Tg (right) mouse showing a focal areas of mild emphysema. Haematoxylin and eosin stain. Original magnification ×40.
(a)
(b)
(c)In addition to neutrophilic inflammation, goblet cell metaplasia and mucus obstruction were a common feature of the airways of adultPI
3
K
γ
WT/βENaC-Tg mice [19]. Since neutrophil products, such as neutrophil elastase, have been implicated in goblet cell metaplasia and mucin hypersecretion in CF [25, 26], we assessed the effects of genetic deletion of PI3Kγ on goblet cell metaplasia. Goblet cells were not observed in PI
3
K
γ
WT and PI
3
K
γ
KO mice; in PI
3
K
γ
KO/βENaC-Tg mice, the goblet cell metaplasia appeared reduced compared to PI
3
K
γ
WT/βENaC-Tg mice; however, this difference was not statistically significant, based on the variability and the number of mice included in our studies (data not shown).


## 3.3. Pharmacological Inhibition of PI3Kγ Reduces Neutrophilic Airway Inflammation in βENaC-Tg Mice

Next we tested effects of pharmacological inhibition of PI3Kγ by using the inhibitor AS-605240 on airway inflammation in βENaC-Tg mice. Treatment of βENaC-Tg mice with AS-605240 but not with vehicle alone reduced neutrophil infiltrates in BALF of βENaC-Tg mice (Figure 3(a)). In contrast, as observed in PI
3
K
γ
KO/βENaC-Tg mice, the PI3Kγ inhibitor had no effect on the recruitment of macrophages or lymphocytes into the lung (Figures 3(b) and 3(c)).Figure 3
Pharmacological inhibition of PI3Kγ decreases neutrophilic airway inflammation in βENaC-Tg mice. Neutrophils (a), lymphocytes (b), and macrophages (c) numbers were determined in BAL fluid of control (WT/Vehicle) and βENaC-Tg mice untreated or treated with the PI3Kγ inhibitor AS-605240 (βENaC-Tg/AS-605240) or with vehicle (βENaC-Tg/Vehicle). Cells are expressed as number per mL of BAL fluid. (a) P
*
*
<
0.01 WT/Vehicleversus βENaC-Tg/Vehicle, P
*
*
<
0.01
βENaC-Tg/Vehicleversus βENaC-Tg/AS-605240 and P
*
<
0.05 WT/Vehicleversus
PI
3
K
γ
WT/βENaC-Tg. (b) P
*
*
<
0.01 WT/Vehicleversus βENaC-Tg/Vehicle and P
*
<
0.05 WT/Vehicleversus
PI
3
K
γ
WT/βENaC-Tg. (c). P
*
*
<
0.01 WT/Vehicleversus βENaC-Tg/Vehicle, P
*
*
<
0.01 WT/Vehicleversus βENaC-Tg/AS-605240, and P
*
<
0.05 WT/Vehicleversus
PI
3
K
γ
WT/βENaC-Tg.
(a)
(b)
(c)


## 4. Discussion

Progressive lung disease is the major cause of morbidity and mortality in CF and is characterized by chronic airway infection and associated airway inflammation leading to irreversible lung destruction and early death [1–3]. Accumulating evidences suggest that CFTR dysfunction impairs mucociliary clearance and bacterial killing as crucial innate defense mechanisms of the lung leading to chronic bacterial infection and nonresolving inflammation in CF airways [3]. The main feature of airway inflammation in CF is a persistent influx of neutrophils that release a variety of oxidants and granule-associated enzymes, thus contributing to the development of lung injury and to the chronicity of pulmonary infection [7–9]. Repeated episodes of exacerbation of chronic infection and inflammation occur during the natural history of the disease, further increasing the structural damage in the CF lung [27, 28]. Therefore, anti-inflammatory therapy, combined with antibiotic therapy, offers a rational approach to prevent chronic lung damage. However, current anti-inflammatory drugs have shown several limits. The use of oral corticosteroids has been limited by severe adverse effects and studies using inhaled corticosteroids in CF have not been particularly successful [8, 9]. In addition, nonsteroidal anti-inflammatory drugs, such as ibuprofen, although revealing beneficial effects in younger CF patients [29], are difficult to dose and thus are not widely used [30]. Likewise, a phase 3 study of the LTB4 receptor antagonist BIIL 284 had been stopped due to adverse effects in the treatment group [31]. An alternative approach to decrease chronic inflammation is to use a more targeted anti-inflammatory therapy directed at reducing neutrophil trafficking in the CF lung. In this context, class I PI3K member, PI3Kγ, has been demonstrated to play a pivotal role in mediating leukocyte recruitment and activation into sites of inflammation [11]. Therefore PI3Kγ may represent an innovative and appropriate target to interfere with the excessive neutrophil-mediated inflammation and damage in CF. Of note, recently developed small-molecule PI3Kγ inhibitors were shown to be effective in suppressing joint inflammation in mouse models of rheumatoid arthritis [32]. In the present study we evaluated the effects of genetic deletion and pharmacologic inhibition of PI3Kγ in the βENaC-Tg mouse as a model of CF lung disease [15, 16, 33]. Such model phenocopies the airway surface dehydration and mucociliary dysfunction characteristic of CF airways. βENaC-Tg mice develop spontaneous CF-like lung disease with early onset goblet cell metaplasia and airway mucus obstruction, reduced bacterial clearance, and chronic neutrophilic inflammation triggering emphysema-like structural lung damage [15, 17, 34, 35]. Genetic deletion of PI3Kγ resulted in decreased neutrophil numbers in BALF of PI
3
K
γ
KO/βENaC-Tg mice, and reduced neutrophilia was associated with reduced emphysematous changes in these mice. Taken together, these data support an important role of PI3Kγ for transmigration of neutrophils from the blood into the airway lumen and a crucial role of neutrophilic airway inflammation in thein vivo pathogenesis of lung damage. Several leukocyte-derived proteases including neutrophil elastase have been shown to cause emphysema in mice [36–38]. Furthermore, previous studies demonstrated that overexpression of several proinflammatory mediators in genetically modified mice induces an imbalance in the pulmonary protease/antiprotease system and emphysema in these mice [39, 40]. Thus, it is likely that neutrophil-dominated chronic pulmonary inflammation and the disruption of protease/antiprotease balance contribute to the development of emphysema in PI
3
K
γ
WT/βENaC-Tg mice. Neutrophil elastase (NE) is the major product of activated neutrophils and has been implicated in the pathogenesis of key features of CF lung disease, such as chronic airway inflammation, mucus hypersecretion, goblet cell metaplasia, and structural damage [41–47]. We hypothesize that deletion of PI3Kγ decreases lung damage through the reduction of neutrophilic inflammation and neutrophil-associated active elastase. Consistently, a recent study demonstrated that NE activity is increased at the surface of airway neutrophils in PI
3
K
γ
WT/βENaC-Tg mice and patients with CF [6] and that genetic deletion of NE results in a significant reduction of emphysema-like changes in PI
3
K
γ
WT/βENaC-Tg mice, suggesting that NE is implicated in emphysema associated with chronic neutrophilic airway inflammationin vivo.Recently, selective PI3Kγ inhibitors have been developed and investigated in different mouse models of chronic inflammation [48–51]. Therefore, we evaluated the efficacy of the PI3Kγ inhibitor AS-605240 on airway inflammation in βENaC-Tg mice; we decided to use AS-605240 for its well characterizedin vivo profile of efficacy and selectivity, indicated by the so far largest number of reports of pharmacological PI3Kγ inhibition in mice [48–53]. We showed that treatment with the PI3Kγ inhibitor decreased the number of neutrophils in BALF of βENaC-Tg mice, thus reproducing the effect observed with the genetic deletion of PI3Kγ. Several technical problems limit the assessment of the increased PI3Kγ activity in βENaC mice; however, the findings that PI
3
K
γ
WT/βENaC-Tg inflamed lungs have more leukocytes than PI
3
K
γ
KO/βENaC-Tg controls are an indirect indication of increased PI3Kγ activity in these mice. Taken together, our data demonstrate the biological efficacy of both genetic deletion and pharmacological inhibition of PI3Kγ in reducing chronic neutrophilic inflammation in CF-like lung diseasein vivo.Whereas blockade of PI3Kγ activity by small-molecule inhibitors may represent a valid approach to modulate excessive leukocyte accumulation in inflamed tissues where leukocyte recruitment is correlated with disease progression, on the other hand increased susceptibility to infection might be a potential side effect of the use of these molecules. In this context, a previous study [54] showed that either gene deletion or pharmacologic inhibition of PI3Kγ in mice infected withS. pneumoniae caused an impaired exudate macrophage recruitment associated with a reduced lung pneumococcal clearance and an impaired resolution/repair process, leading to progressive pneumococcal pneumonia. Thus, whereas pharmacological inhibition of PI3Kγ, eventually in association with antibacterial treatment, may be a viable strategy to inhibit chronic inflammation and limit lung damage in stable CF lung disease, it might have adverse effects on host defense in acute infections when high bacterial burden occurs. In view of a clinical application of PI3Kγ inhibitors, target validation will be an important future aspect to discriminate between specific effects of the drug and potential side effects.


## 5. Conclusions

Neutrophil-dominated airway inflammation has been implicated as a key feature of progressive lung damage in CF. Thus, reducing airway inflammation is a major goal to prevent lung damage and maintain lung function in CF. Current therapeutic strategies that aim to reduce chronic neutrophilic inflammation in the airways of CF patients have been largely unsuccessful. This study shows that genetic deletion and pharmacological inhibition of PI3Kγ decrease neutrophilic airway inflammation and structural lung damage in a mouse model of CF lung disease. These results provide insight into the molecular mechanisms of chronic airway inflammation and suggest a novel treatment strategy to reduce inflammation and lung damage in patients with CF and potentially other neutrophilic airway diseases. Further studies with emerging PI3Kγ inhibitors [49–51] are required to confirm the efficacy of these molecules and exclude their potentially adverse effects on host defense.



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*Source: 545417-2015-06-21.xml*