# Chemical and Functional Characterization of Sarcoplasmic Proteins from Giant Squid (Dosidicus gigas) Mantle

Authors: Rosa Linda Lopez-Enriquez; Victor Manuel Ocano-Higuera; Wilfrido Torres-Arreola; Josafat Marina Ezquerra-Brauer; Enrique Marquez-Rios
Journal: Journal of Chemistry
 (2015)

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## Abstract

Modification of pH and NaCl concentration changed the physicochemical properties of sarcoplasmic proteins (SP) from jumbo squid mantle and consequently their functional properties. Better results of emulsifying activity index (EAI) and foam capacity (FC) were exhibited at pH 11 in NaCl absence due to higher solubility. But better emulsifying stability index (ESI) was obtained at pH 11 in 0.5 M NaCl, while, foaming stability (FS) was better at pH near to isoelectric point (pI). These results suggest that SP from jumbo squid may be a promising ingredient, whose functional properties can be manipulated by changing pH and NaCl concentration.


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## Body

## 1. Introduction

The giant squid (Dosidicus gigas) represents one of the most important fisheries in Mexico in terms of catch volumes. However, it is an underutilized and undervalued resource despite its abundance and its nutritional value [1]. In order to increase its value and promote its consumption, several researches have been carried out focused on obtaining protein concentrates from its mantle. Nonetheless, the conventional process for production thereof generates high volumes of effluent with high content of soluble protein called sarcoplasmic proteins, which can cause contamination problems if they are not treated before disposal [2].Sarcoplasmic proteins represent 20 to 40% of the total muscle proteins. These proteins are globular and exhibit solubility in water and diluted salt solutions and are constituted mainly by enzymes involved in cellular metabolism [3]. There are few studies about the protein composition, structure, and functionality of the sarcoplasmic proteins, in comparison to myofibrillar proteins. Therefore, their study could provide information for use as food ingredients. The above would not only reduce pollution problems, it could also make the use of marine resources more efficient, which is the current trend, not only in fisheries but throughout the food industry.The effect of the sarcoplasmic proteins has been studied on the textural quality of gels made from myofibrillar proteins of various species of fish, obtaining favorable results [4, 5]. Additionally, other functional properties have been explored, such as emulsifying and foaming property [6, 7], demonstrating that sarcoplasmic proteins have potential to be used as ingredients in the food industry.In recent years, various methods have been studied to induce improvement of functional properties of proteins, due to the fact that alternative processes based on extrinsic factors such as pH shift, ionic strength, and temperature may induce conformational changes in proteins, which in turn can improve their functional properties [6]. In this sense, Hemung et al. [8] demonstrated that the sarcoplasmic proteins of sea bream (Nemipterus hexodon), treated in acid and alkaline conditions, followed by neutralization, exhibited conformational changes which improved the emulsifying activity. In another study, Hemung et al. [8] studied the effect of pH, without subsequent neutralization of the sarcoplasmic proteins from the same species, and found that, in acidic conditions, proteins exhibited conformational changes which in turn induced the increased emulsifying activity.Nowadays there are no researches focused on the study of sarcoplasmic proteins from giant squid mantle. Consequently, due to the importance of generating new knowledge about these proteins, which would allow their use as a food ingredient, the objective of this research is to recover sarcoplasmic proteins from giant squid mantle and evaluate the pH and ionic strength effects on the physicochemical and functional properties.


## 2. Materials and Methods

### 2.1. Raw Material

Jumbo squid (D. gigas) was harvested off the coast of Kino Bay, Sonora, Mexico, in June, 2014. Ten specimens were decapitated and gutted on site and were then washed with freshwater at room temperature (25°C). The mantles (experimental samples) were bagged and placed in alternating layers of ice-squid-ice in a portable cooler and transported to the laboratory. Squid mantles exhibited a length of 37.85±4.1 cm and a total weight of 827.5±82.75 g. The elapsed time between capture and reaching the laboratory did not exceed 12 h.


### 2.2. Sarcoplasmic Proteins Preparation

Frozen squid mantle was thawed at 4-5°C for 12 h, minced, and mixed with cold distilled water (≤4°C) at a ratio of 1 : 3 (mince : water). Mixture was then homogenized at 1000 rpm for 1 min using a tissue homogenizer (Wisd; WiseTis HG-15D; Witeg, Germany). The homogenate was centrifuged in a refrigerated centrifuge at 12000 ×g for 20 min at 4°C (Thermo Scientific; Sorvall Biofuge Stratos; MA, USA). The supernatant obtained was considered as the sarcoplasmic proteins (SP) fraction and was analyzed for protein concentration according to the Lowry method [9], using bovine serum albumin as standard.


### 2.3. Modification of pH and NaCl Concentration

Twenty mL aliquots of SP were added to NaCl (0, 0.2, and 0.5 M) and the pH was adjusted to 3.0, 5.0, 7.0, 9.0, or 11.0. Then, the final volume of the homogenates was brought to 25 mL (using cold distilled water) and stirred for 30 min at 4°C. The adjusted solutions were used to determine protein solubility, emulsifying, and foam properties.


### 2.4. Protein Solubility

In order to determine the effect of different pH levels and NaCl concentrations on the solubility of SP, all solutions previously prepared were centrifuged at 12000 ×g for 20 min at 4°C (Thermo Scientific; Sorvall Biofuge Stratos; MA, USA). Protein concentration of the supernatants was analyzed by Lowry method [9]. Protein solubility was expressed as the percentage of protein remaining soluble after centrifugation in relation to the total protein present in SP. Supernatants obtained were used for the determination of protein patterns by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), surface hydrophobicity (S0-PRODAN and S0-ANS), and total sulfhydryl (SH) content.


### 2.5. Electrophoretic Profile (SDS-PAGE)

The electrophoretic profile of each protein system was analyzed by means of polyacrylamide gel electrophoresis (PAGE) using a dissociating sodium dodecyl sulfate (SDS) buffer system in a discontinuous gel (4% stacking gel and 10% separating gel) according to the method of Laemmli [10]. A Mini-PROTEAN 3 Cell Multi-Casting Chamber (Bio-Rad Laboratories, Hercules, CA) was used. Electrophoretic runs were performed at room temperature (25°C) at 80 V. Thirty μg of protein was loaded into each lane of the gels, and a broad range of molecular weight protein standard solutions (Bio-Rad Laboratories, Richmond, CA) containing myosin (200 kDa), beta-galactosidase (116 kDa), phosphorylase b (97 kDa), bovine serum albumin (66 kDa), ovalbumin (45 kDa), and carbonic anhydrase (31 kDa) were used. After electrophoresis, the gel was stained with 0.125% (w/v) Coomassie brilliant blue R-250 in 40% (v/v) methanol and 7% (v/v) acetic acid and the gel was destained with 50% (v/v) methanol and 10% (v/v) acetic acid.


### 2.6. Surface Hydrophobicity (S0)

Surface hydrophobicity (S0) was determined by the method reported by Alizadeh-Pasdar and Li-Chan [11] with slight modifications. Protein solutions were diluted in order to obtain protein concentrations of 0, 0.125, 0.25, 0.5, and 0.1 mg mL−1. Four mL aliquot from each diluted solution was mixed with 20 μL of ANS solution (10 mM phosphate buffer, pH 7) or 10 μL of PRODAN solution (1.4 mM in methanol). ANS samples were set for 10 min and PRODAN samples were set in the dark for 15 min. Subsequently, the fluorescence intensity of the samples was measured using a spectrofluorometer (Agilent Technologies; Cary Eclipse; CA, USA) at excitation/emission wavelength of 390/470 nm and 365/465 for ANS and PRODAN, respectively. The surface hydrophobicity index (S0) was calculated from the regression slope or net relative fluorescence intensity versus protein concentration (%, w/v).


### 2.7. Determination of Total Sulfhydryl (TSH)

Total sulfhydryl (TSH) content, at different pH levels and NaCl concentrations, was determined by the method reported by Tadpitchayangkoon et al. [12] with slight modifications. Protein content of each protein solution was adjusted to 1 mg mL−1. 0.5 mL aliquot of each treatment was mixed with 2 mL of solubilizing buffer (0.2 M Tris-HCl, 8 M urea, 10 mM EDTA, pH 7.0) and 50 μL of Ellman’s reagent (10 mM 5,5′-dinitrobis [2-nitrobenzoic acid], 0.1 M sodium phosphate buffer, pH 7.0). All samples were incubated at 40°C for 25 min. The absorbance was measured at 412 nm using a UV-Vis spectrophotometer (Cary 50; Varian; CA, USA), and SH content was calculated using the extinction coefficient of 13,600 M−1cm−1.


### 2.8. Emulsifying Properties

The emulsifying activity index (EAI) and emulsifying stability index (ESI), at different pH levels and NaCl concentrations, were determined by turbidimetric technique of Pearce and Kinsella [13] with some modifications. Each protein solution was adjusted to 0.5 mg mL−1. Protein solutions were mixed with canola oil at a ratio of 1 : 1 and homogenized at 13500 rpm for 1 min using a tissue homogenizer (Wisd; WiseTis HG-15D; Witeg, Germany). To determine EAI, the emulsions phases were allowed to separate during 1 min and the lower layer was obtained (100 μL) and diluted 40 times with 0.1% of sodium dodecyl sulphate (SDS) and the absorbance was recorded at 500 nm (A500) using a UV-Vis spectrophotometer (Cary 50; Varian; CA, USA). The EAI was calculated using the following equation:(1)τ=2.303×A500×Fl,EAIm2g2=2τϕC,where τ is the turbidity, A500 is the sample absorbance, F is the sample dilution factor (40), and l (0.01 m) is the light path length. The oil volume fractions for emulsions (ϕ) were 0.5, and the protein emulsifier concentration (C) was 0.5 mg mL−1.To determine emulsifying stability index (ESI), aliquots of prepared emulsions (10 mL) were placed inside of 10 mL containers immediately after preparation. At 0 and 10 min following emulsion preparation, 100μL samples were removed and diluted 40-fold prior to turbidity measurements. ESI was calculated from(2)ESImin⁡=τ×ΔtΔτ,where Δτ is the change in turbidity after a time interval Δt (10 min).


### 2.9. Foaming Properties

Foaming capacity (FC) and foam stability (FS) at different pH levels and NaCl concentrations were determined according to the Wild and Clark method [14] with some modifications. Each protein solution was adjusted to 2 mg mL−1. Twenty milliliters of diluted protein solutions was homogenized at 18000 rpm for 1 min using a tissue homogenizer (Wisd; WiseTis HG-15D; Witeg, Germany). The solutions were transferred to a measuring cylinder. The volume of foam at 30 s was calculated, and the volume increase was expressed as percent foaming capacity (FC) and was calculated from(3)FC%=Volume  of  foam  (mL)Initial  liquid  volume  (mL)×100.The foam stability (FS) was calculated as the percentage of foam remaining after 30 min at 25°C, using the following equation:(4)FS%=Volume  of  foam  (mL)  retained  after30min⁡Volume  of  foam  soon  after  whipping  (mL)×100.


### 2.10. Experimental Design and Statistical Analysis

A factorial experiment (5 × 3) was performed to determine the effects of individual factors and their interactions: ph with 5 levels and NaCl concentration with 3. The response variables were protein solubility (%), total sulfhydryl content (TSH, mole 105 g−1), surface hydrophobicity (S0-ANS and S0-PRODAN), emulsifying activity index (EAI, m2g−1), emulsifying stability index (ESI, min), foaming capacity (FC, %), and foam stability (FS, %). All measurements were performed in triplicate, and data were presented as the mean ± standard deviation. In order to determine the statistical differences among treatments, a two-way analysis of variance (ANOVA) was used to compare means with a significance level of P<0.05. Data were analyzed using the JMP statistical software version 10.0.0.


## 2.1. Raw Material

Jumbo squid (D. gigas) was harvested off the coast of Kino Bay, Sonora, Mexico, in June, 2014. Ten specimens were decapitated and gutted on site and were then washed with freshwater at room temperature (25°C). The mantles (experimental samples) were bagged and placed in alternating layers of ice-squid-ice in a portable cooler and transported to the laboratory. Squid mantles exhibited a length of 37.85±4.1 cm and a total weight of 827.5±82.75 g. The elapsed time between capture and reaching the laboratory did not exceed 12 h.


## 2.2. Sarcoplasmic Proteins Preparation

Frozen squid mantle was thawed at 4-5°C for 12 h, minced, and mixed with cold distilled water (≤4°C) at a ratio of 1 : 3 (mince : water). Mixture was then homogenized at 1000 rpm for 1 min using a tissue homogenizer (Wisd; WiseTis HG-15D; Witeg, Germany). The homogenate was centrifuged in a refrigerated centrifuge at 12000 ×g for 20 min at 4°C (Thermo Scientific; Sorvall Biofuge Stratos; MA, USA). The supernatant obtained was considered as the sarcoplasmic proteins (SP) fraction and was analyzed for protein concentration according to the Lowry method [9], using bovine serum albumin as standard.


## 2.3. Modification of pH and NaCl Concentration

Twenty mL aliquots of SP were added to NaCl (0, 0.2, and 0.5 M) and the pH was adjusted to 3.0, 5.0, 7.0, 9.0, or 11.0. Then, the final volume of the homogenates was brought to 25 mL (using cold distilled water) and stirred for 30 min at 4°C. The adjusted solutions were used to determine protein solubility, emulsifying, and foam properties.


## 2.4. Protein Solubility

In order to determine the effect of different pH levels and NaCl concentrations on the solubility of SP, all solutions previously prepared were centrifuged at 12000 ×g for 20 min at 4°C (Thermo Scientific; Sorvall Biofuge Stratos; MA, USA). Protein concentration of the supernatants was analyzed by Lowry method [9]. Protein solubility was expressed as the percentage of protein remaining soluble after centrifugation in relation to the total protein present in SP. Supernatants obtained were used for the determination of protein patterns by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), surface hydrophobicity (S0-PRODAN and S0-ANS), and total sulfhydryl (SH) content.


## 2.5. Electrophoretic Profile (SDS-PAGE)

The electrophoretic profile of each protein system was analyzed by means of polyacrylamide gel electrophoresis (PAGE) using a dissociating sodium dodecyl sulfate (SDS) buffer system in a discontinuous gel (4% stacking gel and 10% separating gel) according to the method of Laemmli [10]. A Mini-PROTEAN 3 Cell Multi-Casting Chamber (Bio-Rad Laboratories, Hercules, CA) was used. Electrophoretic runs were performed at room temperature (25°C) at 80 V. Thirty μg of protein was loaded into each lane of the gels, and a broad range of molecular weight protein standard solutions (Bio-Rad Laboratories, Richmond, CA) containing myosin (200 kDa), beta-galactosidase (116 kDa), phosphorylase b (97 kDa), bovine serum albumin (66 kDa), ovalbumin (45 kDa), and carbonic anhydrase (31 kDa) were used. After electrophoresis, the gel was stained with 0.125% (w/v) Coomassie brilliant blue R-250 in 40% (v/v) methanol and 7% (v/v) acetic acid and the gel was destained with 50% (v/v) methanol and 10% (v/v) acetic acid.


## 2.6. Surface Hydrophobicity (S0)

Surface hydrophobicity (S0) was determined by the method reported by Alizadeh-Pasdar and Li-Chan [11] with slight modifications. Protein solutions were diluted in order to obtain protein concentrations of 0, 0.125, 0.25, 0.5, and 0.1 mg mL−1. Four mL aliquot from each diluted solution was mixed with 20 μL of ANS solution (10 mM phosphate buffer, pH 7) or 10 μL of PRODAN solution (1.4 mM in methanol). ANS samples were set for 10 min and PRODAN samples were set in the dark for 15 min. Subsequently, the fluorescence intensity of the samples was measured using a spectrofluorometer (Agilent Technologies; Cary Eclipse; CA, USA) at excitation/emission wavelength of 390/470 nm and 365/465 for ANS and PRODAN, respectively. The surface hydrophobicity index (S0) was calculated from the regression slope or net relative fluorescence intensity versus protein concentration (%, w/v).


## 2.7. Determination of Total Sulfhydryl (TSH)

Total sulfhydryl (TSH) content, at different pH levels and NaCl concentrations, was determined by the method reported by Tadpitchayangkoon et al. [12] with slight modifications. Protein content of each protein solution was adjusted to 1 mg mL−1. 0.5 mL aliquot of each treatment was mixed with 2 mL of solubilizing buffer (0.2 M Tris-HCl, 8 M urea, 10 mM EDTA, pH 7.0) and 50 μL of Ellman’s reagent (10 mM 5,5′-dinitrobis [2-nitrobenzoic acid], 0.1 M sodium phosphate buffer, pH 7.0). All samples were incubated at 40°C for 25 min. The absorbance was measured at 412 nm using a UV-Vis spectrophotometer (Cary 50; Varian; CA, USA), and SH content was calculated using the extinction coefficient of 13,600 M−1cm−1.


## 2.8. Emulsifying Properties

The emulsifying activity index (EAI) and emulsifying stability index (ESI), at different pH levels and NaCl concentrations, were determined by turbidimetric technique of Pearce and Kinsella [13] with some modifications. Each protein solution was adjusted to 0.5 mg mL−1. Protein solutions were mixed with canola oil at a ratio of 1 : 1 and homogenized at 13500 rpm for 1 min using a tissue homogenizer (Wisd; WiseTis HG-15D; Witeg, Germany). To determine EAI, the emulsions phases were allowed to separate during 1 min and the lower layer was obtained (100 μL) and diluted 40 times with 0.1% of sodium dodecyl sulphate (SDS) and the absorbance was recorded at 500 nm (A500) using a UV-Vis spectrophotometer (Cary 50; Varian; CA, USA). The EAI was calculated using the following equation:(1)τ=2.303×A500×Fl,EAIm2g2=2τϕC,where τ is the turbidity, A500 is the sample absorbance, F is the sample dilution factor (40), and l (0.01 m) is the light path length. The oil volume fractions for emulsions (ϕ) were 0.5, and the protein emulsifier concentration (C) was 0.5 mg mL−1.To determine emulsifying stability index (ESI), aliquots of prepared emulsions (10 mL) were placed inside of 10 mL containers immediately after preparation. At 0 and 10 min following emulsion preparation, 100μL samples were removed and diluted 40-fold prior to turbidity measurements. ESI was calculated from(2)ESImin⁡=τ×ΔtΔτ,where Δτ is the change in turbidity after a time interval Δt (10 min).


## 2.9. Foaming Properties

Foaming capacity (FC) and foam stability (FS) at different pH levels and NaCl concentrations were determined according to the Wild and Clark method [14] with some modifications. Each protein solution was adjusted to 2 mg mL−1. Twenty milliliters of diluted protein solutions was homogenized at 18000 rpm for 1 min using a tissue homogenizer (Wisd; WiseTis HG-15D; Witeg, Germany). The solutions were transferred to a measuring cylinder. The volume of foam at 30 s was calculated, and the volume increase was expressed as percent foaming capacity (FC) and was calculated from(3)FC%=Volume  of  foam  (mL)Initial  liquid  volume  (mL)×100.The foam stability (FS) was calculated as the percentage of foam remaining after 30 min at 25°C, using the following equation:(4)FS%=Volume  of  foam  (mL)  retained  after30min⁡Volume  of  foam  soon  after  whipping  (mL)×100.


## 2.10. Experimental Design and Statistical Analysis

A factorial experiment (5 × 3) was performed to determine the effects of individual factors and their interactions: ph with 5 levels and NaCl concentration with 3. The response variables were protein solubility (%), total sulfhydryl content (TSH, mole 105 g−1), surface hydrophobicity (S0-ANS and S0-PRODAN), emulsifying activity index (EAI, m2g−1), emulsifying stability index (ESI, min), foaming capacity (FC, %), and foam stability (FS, %). All measurements were performed in triplicate, and data were presented as the mean ± standard deviation. In order to determine the statistical differences among treatments, a two-way analysis of variance (ANOVA) was used to compare means with a significance level of P<0.05. Data were analyzed using the JMP statistical software version 10.0.0.


## 3. Results and Discussion

### 3.1. Sarcoplasmic Protein Recovery

The SP solution exhibited a protein concentration of6.59±1.42 mg mL−1 and represented the 14.02±4.21% of total protein content from the mantle. The protein concentration of the SP extract was in the range obtained for Pacific whiting (Merluccius productus) surimi wash-water (5.4–23.4 mg mL−1) [15, 16]. This latter is one of the most utilized species for the manufacture of surimi (Rocha-Estrada et al., 2010). Regarding protein recovery, the SP fraction obtained in this study was lower than that obtained by Sánchez-Alonso et al. [17], who used a 1 : 5 (mantle : water) ratio for the extraction of sarcoplasmic proteins from jumbo squid mantle, reporting a protein recovery of 25–27%, while De la Fuente-Betancourt et al. [18], who used a 1 : 10 (mantle : water) ratio, reported a recovery of 9.61%, which is lesser than that obtained in this study. The recovery of SP fraction from jumbo squid mantle might be affected by factors related to protein extraction as the muscle/water ratio and the centrifugation conditions used [19]. Also, it might be affected by intrinsic (size and chemical composition) and extrinsic aspects (harvest season, temperature, and food availability) of the species [20].


### 3.2. Protein Solubility

Solubility is a critical functional property of proteins, because it is considered as prerequisite for other functionalities, including emulsifying and foaming properties. The effect of pH and NaCl concentration on the solubility of SP is shown in Figure1. The solubility of SP in absence of NaCl showed the typical U-shaped curve as most food proteins, including marine sources [12]. The minimum and the maximum solubility of SP were presented at pH of 5 and 11, respectively. Hence, the isoelectric point (pI) of SP could be close to pH 5.0. The higher solubility of SP at alkaline pH in comparison to acid pH might be due to the amino acid ionization, unfolding, and hydration of protein. Most food proteins are acidic, since the sum of their acid residues (Asp and Glu) is higher than their basic residues (Lys, Arg, and His); therefore, proteins exhibit the minimal solubility at pH 4-5 (isoelectric pH) and maximum at alkaline pH values [4]. In this sense, the presence of higher content of acid than basic residues in proteins from jumbo squid (Dosidicus gigas) mantle has been reported, and this fact matches with the high solubility of SP obtained in this study at pH [21, 22].Figure 1
Effect of pH and NaCl concentration on protein solubility of SP from jumbo squid mantle. Data represent the mean ± standard deviation (n=3).At alkaline pH the solubility was similar. However, at pH 3.0 the solubility was significantly different (P<0.05). It was noted that the solubility was reduced when salt was added, obtaining the minimum with 0.5 M of NaCl. This behavior could be attributed to the interaction of negatively charged chloride ions (Cl−) with positively charged groups (NH3+, from Lys, Arg, and His) of SP, leading to a decrease in electrostatic repulsion and subsequently leading to the aggregation and precipitation [23, 24]. A similar phenomenon has been reported for myofibrillar proteins from Pacific whiting (Merluccius productus) and sarcoplasmic proteins from rockfish (Sebastes flavidus) [5, 24].


### 3.3. Electrophoretic Profile (SDS-PAGE)

In order to evaluate the effect of pH and NaCl concentration on the molecular weights (MW) of proteins present in sarcoplasmic fraction from squid mantle, electrophoretic profiles were determined. SDS-PAGE of the SP is shown in Figure2. Protein bands with MW ranging from 30 to 63.7 were detected, and the corresponding bands 37.5, 38.2, and 44.3 kDa were the most intense. When the NaCl was added to SP system, supernatants obtained at pH 5–11 at all NaCl concentrations (0, 0.2, and 0.5) did not show qualitative differences. The presence of weak bands at pH 3 in NaCl 0.5 M reaffirms the low protein solubility of SP at this condition. Based on solubility results and SDS-PAGE patterns, NaCl concentration appeared to have a negative effect on solubility of SP from jumbo squid mantle at extreme pH. But SP from jumbo squid mantle became more soluble and stable at neutral and at alkaline values than at acid pH values.Figure 2
Protein patterns of SP from jumbo squid in (a) 0 M NaCl, (b) 0.2 M NaCl, and (c) 0.5 M NaCl. The numbers indicate pH while S denotes molecular weight standard.
(a)
(b)
(c)The electrophoretic pattern obtained in this experiment was similar to that obtained by Ezquerra-Brauer et al. [20] for water-soluble proteins (20–66 kDa) from jumbo squid mantle. However, De la Fuente-Betancourt et al. [18] obtained higher MW of SP from mantle of same species (20–97 kDa). As described earlier, the recovery of SP fraction might be affected by intrinsic and extrinsic factors of jumbo squid, as well as by extraction conditions used [19, 25].The proteins present in sarcoplasmic fraction from fish species are related to aerobic metabolism of the fish cells and have exhibited MW of 34, 40, 43, 50, and 94 kDa, which were attributed to glyceraldehyde phosphate dehydrogenase, aldolase, creatine kinase, enolase, and phosphorylase, respectively [3, 12, 26, 27]. Nevertheless, since jumbo squid is a mollusk, it has a quite different SP profile compared to fish species; activity of arginine kinase (40–43 kDa) and octopine dehydrogenase (37–45 kDa) has been reported [28–31]. These are enzymes related to the anaerobic metabolism of mollusks and exhibit MW similar to that obtained in this study for SP fraction from jumbo squid mantle. However, in order to know more about the protein components of SP, further research related to protein characterization is required.


### 3.4. Total Sulfhydryl (SH) Content

Conformational changes of proteins can lead to the exchange of SH/S-S. Thus, total SH content is an indicator of changes in protein conformation and flexibility [20, 21]. Total sulfhydryl (TSH) content of SP varied significantly with pH and NaCl concentration (P<0.05) (Figure 3). The TSH content decreased when pH was changed from 5 to 11 and from 5 to 3; the effect was more pronounced as the NaCl concentration increased. The unfolding of SP induced by pH shift (at both acidic and alkaline pH values) might have induced the exposition of buried SH groups and the consequent S-S formation. It was reported that exchange reactions SH/S-S are favored at alkaline pH [32, 33]. The results of SH content demonstrated that at extreme pH (pH 3 and pH 11) the SH oxidation of SP was induced. Similar findings for myofibrillar proteins from Pacific whiting (Merluccius productus) and sarcoplasmic proteins from striped catfish (Pangasius hypophthalmus) have been reported [11, 34]. On the other hand, the decrease of TSH content by NaCl effect can be attributed to a better protein unfolding, with the consequent exposition of intern functional groups, including the SH groups [22, 35]. The major exposition of SH groups might have allowed the exchange of SH to S-S.Figure 3
Effect of pH and NaCl concentration on total sulfhydryl (TSH) content in SP from jumbo squid mantle. Data represent the mean ± standard deviation (n=3).


### 3.5. Surface Hydrophobicity (S0)

Variations on surface hydrophobicity (S0) denote changes in protein structure/conformation. In this study, surface hydrophobicity of SP was determined at different pH levels and NaCl concentrations, based on ANS and PRODAN probes. Surface hydrophobicity based on the ANS probe (S0-ANS) was significantly affected by pH and NaCl concentration (P<0.05) (Figure 4(a)). Extremely high values of S0-ANS were obtained at pH 3 compared to S0-PRODAN values. It has been reported that at acidic pH values (minor to pH 3.8) S0-ANS reflects not only the hydrophobic interaction between ANS and proteins, but also electrostatic interactions between anionic sulfonate groups of ANS and cationic groups (Lys, Arg, and His) of proteins [10]. Other research works reported the same behavior of ANS at acidic pH values [11, 34]. Therefore, in this experiment, the change of surface hydrophobicity is discussed with more detail in function of PRODAN reagent.Figure 4
Effect of pH and NaCl concentration on the surface hydrophobicity (S0) in SP from jumbo squid mantle, using the probes (a) ANS and (b) PRODAN. Data represent the mean ± standard deviation (n=3).
(a)
(b)S0-PRODAN was also significantly affected by pH and NaCl concentrations (P<0.05) (Figure 4(b)). The S0-PRODAN values of SP in absence of NaCl increased as the pH increased in the range from 5 to 11. The high S0-PRODAN values at alkaline pH (11) were attributed to a higher exposure of buried hydrophobic groups due to the electrostatic repulsion of negative charged residues. In contrast, at pH 5, the minimum S0-PRODAN value was showed, and this might be due to the protein aggregation, because at this pH the minimum solubility was detected, leading to a lower surface hydrophobicity of SP. These results agree with those obtained for myofibrillar proteins from Pacific whiting (Merluccius productus), sarcoplasmic proteins from striped catfish (Pangasius hypophthalmus), and threadfin bream (Nemipterus sp.) [6, 11, 34].It was proved that a positive correlation exists between solubility and surface hydrophobicity of SP, being highly significant withr2 of 0.82, 0.91, and 0.96 for 0.0, 0.2, and 0.5 M of NaCl, respectively. This could be explained as a function of the unfolding of SP induced by pH and NaCl modification and the consequent increase of both solubility and surface hydrophobicity. The effect of NaCl concentration at pH 3 was opposite in comparison to other pH values; however, this behavior was the same as the solubility, and it could be due to the decrease of hydrophobic groups exposed for the SP aggregation, as already explained for SP solubility.


### 3.6. Emulsifying Properties

The effects of pH and NaCl concentration on emulsifying activity index (EAI) and emulsifying stability index (ESI) are shown in Figure5. The EAI value of crude extract of SP (pH 7, 0 M NaCl) obtained in this study (83.32±1.59 m2g−1) was lower than that obtained for sarcoplasmic proteins from threadfin bream (Nemipterus sp.) (~210 m2g−1) [6]. However, this was higher than that reported for lyophilized sarcoplasmic proteins from threadfin bream (Nemipterus hexodon), because an EAI of 46 m2g−1 has been reported [6].Figure 5
Effect of pH and NaCl concentration on (a) emulsifying activity index (EAI) and (b) emulsifying stability index (ESI) of SP from jumbo squid mantle. Data represent the mean ± standard deviation (n=3).
(a)
(b)Figure5(a) shows the EAI for SP as a function of pH and NaCl concentration. EAI of SP was significantly affected by the pH and NaCl concentration (P<0.05), showing the minimum value at pH 5 and the higher at pH 11. Since pH 5 is closer to the isoelectric point, the low EAI was presented due to aggregation and loss of solubility of SP, as a result of the minimum electrostatic repulsion [24]. Yongsawatdigul and Hemung [26] also found that EAI of emulsion stabilized by sarcoplasmic proteins of threadfin bream (Nemipterus sp.) was lowest at its pI (pH 5).At pH 5, the increase in EAI could be due to increase in solubility, caused by the addition of NaCl. At pH lower or higher than 5 the EAI increase can be due to a major solubility. It was noted that, at pH lower or higher than 5, the EAI changes very little; it remains practically unchanged, with pH 11 in absence of NaCl as exception. Kristinsson and Hultin [16] also found that EAI of emulsion stabilized by myofibrillar proteins from cod (Gadus morhua) was higher at pH 11, and this result was well correlated with the increase of surface hydrophobicity and interfacial activity. In contrast, Hemung et al. [8] found that EAI of emulsion stabilized by sarcoplasmic proteins from threadfin bream (Nemipterus sp.) was higher at pH 3 (610 m2g−1) than at pH 12 (363 m2g−1), and these results were not correlated with S0-PRODAN values. Hence, it can be mentioned that the EAI of proteins is species dependent.The addition of NaCl to the SP system at pH 11 decreases the EAI, which could be related to the increase of hydrophobic groups exposed (S0-PRODAN) and the decrease in solubility with the increase of NaCl concentration, indicating that the hydrophobic/hydrophilic balance of SP was affected. This balance is necessary because proteins have to interact with both oil and water; thus, the high exposition of hydrophobic groups and the possible major rigidity of SP by the disulfide bonds (S-S) formation could have induced the decrease of EAI of emulsion at pH 11. Yuliana et al. [36] also found that EAI of emulsion stabilized by proteins isolated from cashew nut shell (Cashew variety Venguria-4) was decreasing with the increase of NaCl concentration (0–2 M). In contrast, Zhang et al. [37] found that EAI of emulsion stabilized by protein concentrate from chickpea (Cicer arietinum L.) was increased with the increase of NaCl concentration (0-1 M), which was correlated with the increase of hydrophobicity.The ESI value of crude extract of SP (pH 7) obtained in this study was73.12±14.57 min. This value was higher than those reported for sarcoplasmic proteins from rohu (Labeo rohita, 52 min) and from threadfin bream (Nemipterus hexodon, 63 min) [8, 38]. Figure 5(b) shows ESI values for SP as a function of pH and NaCl concentration. ESI was significantly affected by the pH and NaCl concentration (P<0.05). The graph indicates that as pH increases ESI increased independently of the NaCl concentration, being significantly higher at pH 11. The minimum ESI value was at pH 3 in the absence of NaCl, whereas the maximum value was reached at pH 11 in presence of 0.5 M NaCl. The increase of ESI at pH 11 regarding SP at pH 7 was 243.31%. This might be due to the large repulsion, which prevents the coalesce [39]. At pH 11 and 0.5 M of NaCl the highest surface hydrophobicity and the lowest SH content were present, the latter indicating that the major disulfide (S–S) bonds formation occurred under these conditions. Therefore, the SP, once absorbed, might stabilize interactions between proteins that form the interface [15, 40].


### 3.7. Foaming Properties

The effects of pH and NaCl concentration on foaming capacity (FC) and foam stability (FS) are shown in Figures6(a) and 6(b), respectively. The FC value of crude extract of SP (pH 7, 0 M NaCl) obtained in this study was 57.5±2.5%. This value was higher than that reported for sarcoplasmic proteins from rohu (Labeo rohita, 41.33%) using a solution with a protein concentration of 2.5 mg mL−1, similar to the one used in this study (2 mg mL−1) [38], and was lower than that reported for sarcoplasmic proteins from threadfin bream (Nemipterus hexodon, 165%) [6].Figure 6
Effect of pH and NaCl concentration on (a) foaming capacity (FC) and (b) foam stability (FS) of SP from jumbo squid mantle. Data represent the mean ± standard deviation (n=3).
(a)
(b)The FC of SP was significantly affected by the pH and NaCl concentration (P<0.05) (Figure 6(a)). The behavior of FC as a function of pH showed a similar tendency as EAI. In absence of NaCl, the FC was found to be the lowest at pH 5 (24.11±3.65%) and the highest at pH 11 (87.84±1.25%). At pH 5, the low FC could be related to low solubility due to the fact that at this pH low electrostatic repulsion occurs causing formation of aggregates and precipitation, affecting its interfacial activity [5, 12]. The highest FC at pH 11 could be related, as well as EAI, to the high protein solubility and hydrophobicity of SP. Krasaechol et al. [6] reported that the FC and FS are related to a high hydrophobicity. Yet, the results obtained for FC, in function of pH, did not correlate well with surface hydrophobicity and solubility of SP.As well as the emulsifying properties, foaming properties of food proteins also are better at pH values away from their isoelectric point (pI), especially at alkaline pH values. Similar results have been found by Rocha-Estrada et al. [41] and Galvez-Rongel et al. [42]; this research found better FC values for proteins from jumbo squid (Dosidicus gigas) mantle treated at alkaline pH values [3].The increment of NaCl concentration decreased the FC values to pH 11, possible due to the higher exposition of hydrophobic groups and decrease of solubility, with the consequent change of hydrophobicity/hydrophobicity balance of the SP, as occurred in EAI. Rocha-Estrada et al. [41] found that the FC of foams stabilized by proteins from jumbo squid (Dosidicus gigas) decreased with the increase of NaCl concentration (0–0.4 M). Yuliana et al. [36] also found that FC stabilized by proteins isolated from cashew nut shell (Cashew variety Venguria-4) decreased with the increase of NaCl concentration (0–2 M).Figure6(b) shows the results of FS (P<0.05). It was detected that FS was affected by the pH and did not follow the obtained tendency of the other functional properties (EAI, ESI, and FC). Furthermore, all pH values presented irregular FS values in function of NaCl concentration. In NaCl absence, FS was found to be the lowest at pH 3 (33.2±5.02%) and the highest at pH 7 (97.76±0.85%). No significant differences (P<0.05) were found at pH 5 by effect of NaCl concentrations. The best results were obtained at pH 5 and pH 7; at higher or lower pH values the tendency was to decrease. This indicates that pH 7 could be the best option to make foams. Our results differ from those obtained by Rocha-Estrada et al. [41] who working with myofibrillar proteins from jumbo squid (Dosidicus gigas) reported that alkaline pH values (10–12) presented the highest FS values, when NaCl was added (0–0.4 M). Nonetheless, many studies have shown that stability is better at pH close to pI. With pH values near to pI, the absence of repulsive interactions between proteins promotes attractive interactions. Thus, it promotes the formation of a viscoelastic layer in the interface air/water [15].


## 3.1. Sarcoplasmic Protein Recovery

The SP solution exhibited a protein concentration of6.59±1.42 mg mL−1 and represented the 14.02±4.21% of total protein content from the mantle. The protein concentration of the SP extract was in the range obtained for Pacific whiting (Merluccius productus) surimi wash-water (5.4–23.4 mg mL−1) [15, 16]. This latter is one of the most utilized species for the manufacture of surimi (Rocha-Estrada et al., 2010). Regarding protein recovery, the SP fraction obtained in this study was lower than that obtained by Sánchez-Alonso et al. [17], who used a 1 : 5 (mantle : water) ratio for the extraction of sarcoplasmic proteins from jumbo squid mantle, reporting a protein recovery of 25–27%, while De la Fuente-Betancourt et al. [18], who used a 1 : 10 (mantle : water) ratio, reported a recovery of 9.61%, which is lesser than that obtained in this study. The recovery of SP fraction from jumbo squid mantle might be affected by factors related to protein extraction as the muscle/water ratio and the centrifugation conditions used [19]. Also, it might be affected by intrinsic (size and chemical composition) and extrinsic aspects (harvest season, temperature, and food availability) of the species [20].


## 3.2. Protein Solubility

Solubility is a critical functional property of proteins, because it is considered as prerequisite for other functionalities, including emulsifying and foaming properties. The effect of pH and NaCl concentration on the solubility of SP is shown in Figure1. The solubility of SP in absence of NaCl showed the typical U-shaped curve as most food proteins, including marine sources [12]. The minimum and the maximum solubility of SP were presented at pH of 5 and 11, respectively. Hence, the isoelectric point (pI) of SP could be close to pH 5.0. The higher solubility of SP at alkaline pH in comparison to acid pH might be due to the amino acid ionization, unfolding, and hydration of protein. Most food proteins are acidic, since the sum of their acid residues (Asp and Glu) is higher than their basic residues (Lys, Arg, and His); therefore, proteins exhibit the minimal solubility at pH 4-5 (isoelectric pH) and maximum at alkaline pH values [4]. In this sense, the presence of higher content of acid than basic residues in proteins from jumbo squid (Dosidicus gigas) mantle has been reported, and this fact matches with the high solubility of SP obtained in this study at pH [21, 22].Figure 1
Effect of pH and NaCl concentration on protein solubility of SP from jumbo squid mantle. Data represent the mean ± standard deviation (n=3).At alkaline pH the solubility was similar. However, at pH 3.0 the solubility was significantly different (P<0.05). It was noted that the solubility was reduced when salt was added, obtaining the minimum with 0.5 M of NaCl. This behavior could be attributed to the interaction of negatively charged chloride ions (Cl−) with positively charged groups (NH3+, from Lys, Arg, and His) of SP, leading to a decrease in electrostatic repulsion and subsequently leading to the aggregation and precipitation [23, 24]. A similar phenomenon has been reported for myofibrillar proteins from Pacific whiting (Merluccius productus) and sarcoplasmic proteins from rockfish (Sebastes flavidus) [5, 24].


## 3.3. Electrophoretic Profile (SDS-PAGE)

In order to evaluate the effect of pH and NaCl concentration on the molecular weights (MW) of proteins present in sarcoplasmic fraction from squid mantle, electrophoretic profiles were determined. SDS-PAGE of the SP is shown in Figure2. Protein bands with MW ranging from 30 to 63.7 were detected, and the corresponding bands 37.5, 38.2, and 44.3 kDa were the most intense. When the NaCl was added to SP system, supernatants obtained at pH 5–11 at all NaCl concentrations (0, 0.2, and 0.5) did not show qualitative differences. The presence of weak bands at pH 3 in NaCl 0.5 M reaffirms the low protein solubility of SP at this condition. Based on solubility results and SDS-PAGE patterns, NaCl concentration appeared to have a negative effect on solubility of SP from jumbo squid mantle at extreme pH. But SP from jumbo squid mantle became more soluble and stable at neutral and at alkaline values than at acid pH values.Figure 2
Protein patterns of SP from jumbo squid in (a) 0 M NaCl, (b) 0.2 M NaCl, and (c) 0.5 M NaCl. The numbers indicate pH while S denotes molecular weight standard.
(a)
(b)
(c)The electrophoretic pattern obtained in this experiment was similar to that obtained by Ezquerra-Brauer et al. [20] for water-soluble proteins (20–66 kDa) from jumbo squid mantle. However, De la Fuente-Betancourt et al. [18] obtained higher MW of SP from mantle of same species (20–97 kDa). As described earlier, the recovery of SP fraction might be affected by intrinsic and extrinsic factors of jumbo squid, as well as by extraction conditions used [19, 25].The proteins present in sarcoplasmic fraction from fish species are related to aerobic metabolism of the fish cells and have exhibited MW of 34, 40, 43, 50, and 94 kDa, which were attributed to glyceraldehyde phosphate dehydrogenase, aldolase, creatine kinase, enolase, and phosphorylase, respectively [3, 12, 26, 27]. Nevertheless, since jumbo squid is a mollusk, it has a quite different SP profile compared to fish species; activity of arginine kinase (40–43 kDa) and octopine dehydrogenase (37–45 kDa) has been reported [28–31]. These are enzymes related to the anaerobic metabolism of mollusks and exhibit MW similar to that obtained in this study for SP fraction from jumbo squid mantle. However, in order to know more about the protein components of SP, further research related to protein characterization is required.


## 3.4. Total Sulfhydryl (SH) Content

Conformational changes of proteins can lead to the exchange of SH/S-S. Thus, total SH content is an indicator of changes in protein conformation and flexibility [20, 21]. Total sulfhydryl (TSH) content of SP varied significantly with pH and NaCl concentration (P<0.05) (Figure 3). The TSH content decreased when pH was changed from 5 to 11 and from 5 to 3; the effect was more pronounced as the NaCl concentration increased. The unfolding of SP induced by pH shift (at both acidic and alkaline pH values) might have induced the exposition of buried SH groups and the consequent S-S formation. It was reported that exchange reactions SH/S-S are favored at alkaline pH [32, 33]. The results of SH content demonstrated that at extreme pH (pH 3 and pH 11) the SH oxidation of SP was induced. Similar findings for myofibrillar proteins from Pacific whiting (Merluccius productus) and sarcoplasmic proteins from striped catfish (Pangasius hypophthalmus) have been reported [11, 34]. On the other hand, the decrease of TSH content by NaCl effect can be attributed to a better protein unfolding, with the consequent exposition of intern functional groups, including the SH groups [22, 35]. The major exposition of SH groups might have allowed the exchange of SH to S-S.Figure 3
Effect of pH and NaCl concentration on total sulfhydryl (TSH) content in SP from jumbo squid mantle. Data represent the mean ± standard deviation (n=3).


## 3.5. Surface Hydrophobicity (S0)

Variations on surface hydrophobicity (S0) denote changes in protein structure/conformation. In this study, surface hydrophobicity of SP was determined at different pH levels and NaCl concentrations, based on ANS and PRODAN probes. Surface hydrophobicity based on the ANS probe (S0-ANS) was significantly affected by pH and NaCl concentration (P<0.05) (Figure 4(a)). Extremely high values of S0-ANS were obtained at pH 3 compared to S0-PRODAN values. It has been reported that at acidic pH values (minor to pH 3.8) S0-ANS reflects not only the hydrophobic interaction between ANS and proteins, but also electrostatic interactions between anionic sulfonate groups of ANS and cationic groups (Lys, Arg, and His) of proteins [10]. Other research works reported the same behavior of ANS at acidic pH values [11, 34]. Therefore, in this experiment, the change of surface hydrophobicity is discussed with more detail in function of PRODAN reagent.Figure 4
Effect of pH and NaCl concentration on the surface hydrophobicity (S0) in SP from jumbo squid mantle, using the probes (a) ANS and (b) PRODAN. Data represent the mean ± standard deviation (n=3).
(a)
(b)S0-PRODAN was also significantly affected by pH and NaCl concentrations (P<0.05) (Figure 4(b)). The S0-PRODAN values of SP in absence of NaCl increased as the pH increased in the range from 5 to 11. The high S0-PRODAN values at alkaline pH (11) were attributed to a higher exposure of buried hydrophobic groups due to the electrostatic repulsion of negative charged residues. In contrast, at pH 5, the minimum S0-PRODAN value was showed, and this might be due to the protein aggregation, because at this pH the minimum solubility was detected, leading to a lower surface hydrophobicity of SP. These results agree with those obtained for myofibrillar proteins from Pacific whiting (Merluccius productus), sarcoplasmic proteins from striped catfish (Pangasius hypophthalmus), and threadfin bream (Nemipterus sp.) [6, 11, 34].It was proved that a positive correlation exists between solubility and surface hydrophobicity of SP, being highly significant withr2 of 0.82, 0.91, and 0.96 for 0.0, 0.2, and 0.5 M of NaCl, respectively. This could be explained as a function of the unfolding of SP induced by pH and NaCl modification and the consequent increase of both solubility and surface hydrophobicity. The effect of NaCl concentration at pH 3 was opposite in comparison to other pH values; however, this behavior was the same as the solubility, and it could be due to the decrease of hydrophobic groups exposed for the SP aggregation, as already explained for SP solubility.


## 3.6. Emulsifying Properties

The effects of pH and NaCl concentration on emulsifying activity index (EAI) and emulsifying stability index (ESI) are shown in Figure5. The EAI value of crude extract of SP (pH 7, 0 M NaCl) obtained in this study (83.32±1.59 m2g−1) was lower than that obtained for sarcoplasmic proteins from threadfin bream (Nemipterus sp.) (~210 m2g−1) [6]. However, this was higher than that reported for lyophilized sarcoplasmic proteins from threadfin bream (Nemipterus hexodon), because an EAI of 46 m2g−1 has been reported [6].Figure 5
Effect of pH and NaCl concentration on (a) emulsifying activity index (EAI) and (b) emulsifying stability index (ESI) of SP from jumbo squid mantle. Data represent the mean ± standard deviation (n=3).
(a)
(b)Figure5(a) shows the EAI for SP as a function of pH and NaCl concentration. EAI of SP was significantly affected by the pH and NaCl concentration (P<0.05), showing the minimum value at pH 5 and the higher at pH 11. Since pH 5 is closer to the isoelectric point, the low EAI was presented due to aggregation and loss of solubility of SP, as a result of the minimum electrostatic repulsion [24]. Yongsawatdigul and Hemung [26] also found that EAI of emulsion stabilized by sarcoplasmic proteins of threadfin bream (Nemipterus sp.) was lowest at its pI (pH 5).At pH 5, the increase in EAI could be due to increase in solubility, caused by the addition of NaCl. At pH lower or higher than 5 the EAI increase can be due to a major solubility. It was noted that, at pH lower or higher than 5, the EAI changes very little; it remains practically unchanged, with pH 11 in absence of NaCl as exception. Kristinsson and Hultin [16] also found that EAI of emulsion stabilized by myofibrillar proteins from cod (Gadus morhua) was higher at pH 11, and this result was well correlated with the increase of surface hydrophobicity and interfacial activity. In contrast, Hemung et al. [8] found that EAI of emulsion stabilized by sarcoplasmic proteins from threadfin bream (Nemipterus sp.) was higher at pH 3 (610 m2g−1) than at pH 12 (363 m2g−1), and these results were not correlated with S0-PRODAN values. Hence, it can be mentioned that the EAI of proteins is species dependent.The addition of NaCl to the SP system at pH 11 decreases the EAI, which could be related to the increase of hydrophobic groups exposed (S0-PRODAN) and the decrease in solubility with the increase of NaCl concentration, indicating that the hydrophobic/hydrophilic balance of SP was affected. This balance is necessary because proteins have to interact with both oil and water; thus, the high exposition of hydrophobic groups and the possible major rigidity of SP by the disulfide bonds (S-S) formation could have induced the decrease of EAI of emulsion at pH 11. Yuliana et al. [36] also found that EAI of emulsion stabilized by proteins isolated from cashew nut shell (Cashew variety Venguria-4) was decreasing with the increase of NaCl concentration (0–2 M). In contrast, Zhang et al. [37] found that EAI of emulsion stabilized by protein concentrate from chickpea (Cicer arietinum L.) was increased with the increase of NaCl concentration (0-1 M), which was correlated with the increase of hydrophobicity.The ESI value of crude extract of SP (pH 7) obtained in this study was73.12±14.57 min. This value was higher than those reported for sarcoplasmic proteins from rohu (Labeo rohita, 52 min) and from threadfin bream (Nemipterus hexodon, 63 min) [8, 38]. Figure 5(b) shows ESI values for SP as a function of pH and NaCl concentration. ESI was significantly affected by the pH and NaCl concentration (P<0.05). The graph indicates that as pH increases ESI increased independently of the NaCl concentration, being significantly higher at pH 11. The minimum ESI value was at pH 3 in the absence of NaCl, whereas the maximum value was reached at pH 11 in presence of 0.5 M NaCl. The increase of ESI at pH 11 regarding SP at pH 7 was 243.31%. This might be due to the large repulsion, which prevents the coalesce [39]. At pH 11 and 0.5 M of NaCl the highest surface hydrophobicity and the lowest SH content were present, the latter indicating that the major disulfide (S–S) bonds formation occurred under these conditions. Therefore, the SP, once absorbed, might stabilize interactions between proteins that form the interface [15, 40].


## 3.7. Foaming Properties

The effects of pH and NaCl concentration on foaming capacity (FC) and foam stability (FS) are shown in Figures6(a) and 6(b), respectively. The FC value of crude extract of SP (pH 7, 0 M NaCl) obtained in this study was 57.5±2.5%. This value was higher than that reported for sarcoplasmic proteins from rohu (Labeo rohita, 41.33%) using a solution with a protein concentration of 2.5 mg mL−1, similar to the one used in this study (2 mg mL−1) [38], and was lower than that reported for sarcoplasmic proteins from threadfin bream (Nemipterus hexodon, 165%) [6].Figure 6
Effect of pH and NaCl concentration on (a) foaming capacity (FC) and (b) foam stability (FS) of SP from jumbo squid mantle. Data represent the mean ± standard deviation (n=3).
(a)
(b)The FC of SP was significantly affected by the pH and NaCl concentration (P<0.05) (Figure 6(a)). The behavior of FC as a function of pH showed a similar tendency as EAI. In absence of NaCl, the FC was found to be the lowest at pH 5 (24.11±3.65%) and the highest at pH 11 (87.84±1.25%). At pH 5, the low FC could be related to low solubility due to the fact that at this pH low electrostatic repulsion occurs causing formation of aggregates and precipitation, affecting its interfacial activity [5, 12]. The highest FC at pH 11 could be related, as well as EAI, to the high protein solubility and hydrophobicity of SP. Krasaechol et al. [6] reported that the FC and FS are related to a high hydrophobicity. Yet, the results obtained for FC, in function of pH, did not correlate well with surface hydrophobicity and solubility of SP.As well as the emulsifying properties, foaming properties of food proteins also are better at pH values away from their isoelectric point (pI), especially at alkaline pH values. Similar results have been found by Rocha-Estrada et al. [41] and Galvez-Rongel et al. [42]; this research found better FC values for proteins from jumbo squid (Dosidicus gigas) mantle treated at alkaline pH values [3].The increment of NaCl concentration decreased the FC values to pH 11, possible due to the higher exposition of hydrophobic groups and decrease of solubility, with the consequent change of hydrophobicity/hydrophobicity balance of the SP, as occurred in EAI. Rocha-Estrada et al. [41] found that the FC of foams stabilized by proteins from jumbo squid (Dosidicus gigas) decreased with the increase of NaCl concentration (0–0.4 M). Yuliana et al. [36] also found that FC stabilized by proteins isolated from cashew nut shell (Cashew variety Venguria-4) decreased with the increase of NaCl concentration (0–2 M).Figure6(b) shows the results of FS (P<0.05). It was detected that FS was affected by the pH and did not follow the obtained tendency of the other functional properties (EAI, ESI, and FC). Furthermore, all pH values presented irregular FS values in function of NaCl concentration. In NaCl absence, FS was found to be the lowest at pH 3 (33.2±5.02%) and the highest at pH 7 (97.76±0.85%). No significant differences (P<0.05) were found at pH 5 by effect of NaCl concentrations. The best results were obtained at pH 5 and pH 7; at higher or lower pH values the tendency was to decrease. This indicates that pH 7 could be the best option to make foams. Our results differ from those obtained by Rocha-Estrada et al. [41] who working with myofibrillar proteins from jumbo squid (Dosidicus gigas) reported that alkaline pH values (10–12) presented the highest FS values, when NaCl was added (0–0.4 M). Nonetheless, many studies have shown that stability is better at pH close to pI. With pH values near to pI, the absence of repulsive interactions between proteins promotes attractive interactions. Thus, it promotes the formation of a viscoelastic layer in the interface air/water [15].


## 4. Conclusions

Conformational changes of sarcoplasmic proteins from jumbo squid mantle were influenced by pH and NaCl concentration and, consequently, their functional properties. Proteins presented the highest solubility and hydrophobicity in absence of NaCl at pH 11 resulting in an increase of emulsifying and foaming capacity. The emulsifying stability was better at pH 11 and was enhanced by the increase of NaCl, due to the hydrophobic interactions on interface oil/water. However, foaming stability was enhanced by the absence of electrostatic repulsions at pH near to pI. These proteins have shown that they could be used as food ingredients and their functional properties can be improved by changing pH and/or NaCl concentration, thus contributing to a better use of this fishery resource.



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*Source: 538721-2015-08-26.xml*