# Evaluation of Aromatic Plants and Compounds Used to  Fight Multidrug Resistant Infections

Authors: Ramar Perumal Samy; Jayapal Manikandan; Mohammed Al Qahtani
Journal: Evidence-Based Complementary and Alternative Medicine
 (2013)

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## Abstract

Traditional medicine plays a vital role for primary health care in India, where it is widely practiced to treat various ailments. Among those obtained from the healers, 78 medicinal plants were scientifically evaluated for antibacterial activity. Methanol extract of plants (100μg of residue) was tested against the multidrug resistant (MDR) Gram-negative and Gram-positive bacteria. Forty-seven plants showed strong activity against Burkholderia pseudomallei (strain TES and KHW) and Staphylococcus aureus, of which Tragia involucrata L., Citrus acida Roxb. Hook.f., and Aegle marmelos (L.) Correa ex Roxb. showed powerful inhibition of bacteria. Eighteen plants displayed only a moderate effect, while six plants failed to provide any evidence of inhibition against the tested bacteria. Purified compounds showed higher antimicrobial activity than crude extracts. The compounds showed less toxic effect to the human skin fibroblasts (HEPK) cells than their corresponding aromatic fractions. Phytochemical screening indicates that the presence of various secondary metabolites may be responsible for this activity. Most of the plant extracts contained high levels of phenolic or polyphenolic compounds and exhibited activity against MDR pathogens. In conclusion, plants are promising agents that deserve further exploration. Lead molecules available from such extracts may serve as potential antimicrobial agents for future drug development to combat diseases caused by the MDR bacterial strains as reported in this study.


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## Body

## 1. Introduction

Treatment of infections is compromised worldwide by the emergence of bacteria that are resistant to multiple antibiotics [1]. New and emerging drug resistance bacteria strains, particularly methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant enterococci (VRE), Mycobacterium tuberculosis (MTB), and multidrug resistance (MDR) Gram-negative bacteria, are increasing worldwide and add to the gravity of the situation [2]. S. aureus cause a variety of syndromes such as food poisoning, toxic shock syndrome, skin lesions [3], hyperproliferative skin disease [4], and atopic dermatitis [5, 6]. Community-acquired pneumonia caused by Streptococcus pneumoniae, Klebsiella pneumonia,andS. aureus accounts for significant mortality in Southeast Asia [7]. Melioidosis has been recognized as an important human infection caused by Burkholderia pseudomallei in Singapore, Malaysia, Thailand, and Northern Australia [8, 9]. Cases have also been reported from some other tropical and subtropical regions like Africa and America, and a number of cases in man has recently been reported to increase in China, Taiwan, and South India [10, 11]. Infection with antibiotic resistant bacteria negatively impacts on public health, due to an increased incidence of treatment failure and severity of diseases. Development of resistant bacteria due to the chromosomal mutations is more commonly associated with the horizontal transfer of resistance determinants borne on mobile genetic elements [12]. B. pseudomallei is intrinsically resistant to many antibiotics [13, 14]. Considering the higher cost for producing synthetic drugs and the various side effects associated with their use, the need to search for alternative agents from medicinal plants and essential oils used in folklore medicine is further justified to overcome these issues. In India, there are about 550 tribal communities covered under 227 ethnic groups residing in about 5000 villages throughout different forest and vegetation regions [15]. India is one of the world’s 12 megabiodiversity countries [16, 17]. Plant derived medicines have played a major role in human societies throughout the history and prehistory of mankind [18]. The traditional healers (traditional physicians) or medicinemen have a long history of their own diagnostic and treatment system, which they have acquired from their ancestors [19]. Approximately 80% of the world population still relies on traditional medicine for the treatment of common diseases [20–22]. Medicinal plants thus offer significant potential for the development of novel antibacterial therapies and adjunct treatments [23]. Plant derived drugs serve as a prototype to develop more effective and less toxic medicines. In previous studies, few attempts were made to confirm the antimicrobial activity of some indigenous medicinal plants [24, 25]. Not only extracts of various medicinal plants but also essential oils and their constituents have been investigated for their antimicrobial properties against bacteria and fungi [26–28].The principal compounds from the leaves showed a better antibacterial activity againstP. aeruginosa and B. subtilis bacteria and a significant antifungal activity on C. albicans[29]. The essential oil from R. officinalis (alpha pinene/verbenone/bornyl acetate) was found to be more sensitive to the Gram-positive bacteria (MIC 2.5–4 mg/mL) than to the Gram-negative bacteria [30]. Several essential oils kill bacteria by damaging the cell membrane structure and inhibiting their membrane function [31]. Because of the antimicrobial potency of plant extracts and oils, they become a rich source of raw materials for many biotechnological and pharmaceutical industries for the development of therapeutic drugs. The increasing trend in the use of aromatic plants and essential oils in food, cosmetic, and pharmaceutical industries suggests that a systematic study of traditional medicinal plants is very important in order to find active compounds from such sources [32–34]. The purpose of this study is to survey and investigate popular medicinal aromatic plants and their essential oils with a view to fight against multidrug resistant human pathogens. In the present study, 71 plant species were selected on the basis of the available medicinal information and screened for their in vitro antimicrobial efficacy against bacteria.


## 2. Materials and Methods

### 2.1. Ethnomedicinal Survey and Collection of Plants

Ethnomedicinal surveys were conducted during March 1998 and July 2001 from various tribal localities (Kolli hills, Kalrayan hills, Pachamalai, Javadi hills, Mundanthurai) of Eastern and Western Ghats, Tamil Nadu, India. For ethnobotanical studies, questioners were used to collect the general information on the tribes, and the key information on medicinal details was collected through interviews. The medicinal plants were identified by a taxonomist using the standard Flora of Tamil Nadu Carnatic [35], and the voucher specimens were deposited in the department’s herbarium at the Entomology Research Institute, Loyola College, Chennai, India.


### 2.2. Preparation of Plant Extracts

Using a Soxhlet apparatus, the shade-dried and powdered plant materials (200 g of each) were extracted with 1000 mL of methanol (CH3OH) for 10 h. The collected methanol extracts were filtered (Whatman no. 1 filter paper) and evaporated with a rotary evaporator and freeze dryer (lyophilized) to obtain the crude extracts (Buchi, Labortechnik AG, Switzerland). The dried crude extracts were stored at 4°C for antimicrobial assays [34].


### 2.3. Culture of Microorganisms

The following Gram-negative:Burkholderia pseudomallei (TES21), Burkholderia pseudomallei (KHW22), Klebsiella pneumoniae (ATCC15380), Klebsiella pneumoniae, Pseudomonas aeruginosa (ATCC27853), Vibrio damsela, and Salmonella typhi (ATCC51812) and Gram-positive: Staphylococcus aureus (ATCC 29213), Streptococcus pyogenes, and Streptococcus pneumoniae (ATCC49619) microorganisms were used for cultures. B. pseudomallei bacterial strains such as KHW and TES were isolated from the patient samples obtained from the Department of Microbiology, NUS, Singapore. The strains were subcultured on 20 mL Tryptic Soy (TS) and Mueller Hinton (MH) agar plates (pH 7.4) and incubated overnight at 37°C before use.


### 2.4. Antimicrobial Activity

The standard bacterial cultures were stored at −70°C, subcultured on 20 mL MH and TS agar plates (pH 7.4), and incubated overnight at 37°C prior to use. The antimicrobial property was tested using the disc-diffusion method [36]. Five young colonies of each strain of bacteria taken from their respective cultures grown overnight on TS agar plates (Oxoid limited, Wode Road, Basingstoke, Hants, England, UK) were suspended in 5 mL of sterile saline (0.9%), and the density of the suspension was adjusted to approximately 3×108 colony forming unit (CFU). The swab was used to inoculate the dried surface of TS agar plate by streaking four times over the surface of the agar and rotating the plate approximately 90°C to ensure an even distribution of the inoculums. The medium was allowed to dry for about 3 min before adding a 6 millimeter in diameter (mm) sterile paper disc (Becton Dickinson, USA) on the surface. Each disc was tapped gently down onto the agar to provide a uniform contact. Lyophilized residue (100 μg/mL) of each plant extracts and purified fractions was weighed and dissolved in 1 mL of water, and 20 μL of the extracts and oils (containing 100 μg of residue) were applied on each disc (3 replicates), while the sterile blank disc served as a normal control. The antimicrobial effect of the extracts on the clinical isolates was determined in comparison with the reference antibiotics (chloramphenicol 30 μg/disc and ceftazidime 30 μg/disc), which were used as positive controls. The plates were incubated at 37°C for 24 h, and the inhibition zones were measured and calculated.


### 2.5. Minimum Inhibitory Concentrations (MICs) Assay

MICs were evaluated based on thein vitro screening of 16 purified fractions that were found to have potent antimicrobial activity. Broth dilution method was used for the MIC assay with some slight modifications as recommend by the NCCLS [37]. Two-fold serial dilutions of all the fractionated compounds were made with MH and TS broth in microtiter plate wells to adjust the final concentration from 7.8 to 250 μg/mL, while wells containing the broth alone without any sample served as a control. Three replicates (n=3) were used for each dilution and culture containing approximately 1×105 CFU/mL. The plates were incubated at 37°C for 24 h, and the absorbance was measured at 560 nm.


### 2.6. Cytotoxicity Assay

The cytotoxic effects of various extracts were tested by MTT assay [38] using human skin fibroblast HEPK cells. The toxic effect of plant extracts and essential oils was assayed on human skin fibroblast (HEPK) cell proliferation in 96-well microtitre plates. Confluent cells (5×106 cells per well) were incubated with extracts and oils for 24 h, and the percentage inhibitory concentration (IC50) was determined.


### 2.7. Phytochemical Analysis of Plant Extracts

The most active extracts were used for purification of antimicrobial compounds [39]. 100 g each of the plant powder was percolated with 500 mL of 4% aqueous HCl (adjusted to pH 2) and heated at 50°C for 3 h. The extract was washed with 2×250 mL of diethyl ether, and the organic phase evaporated to dryness using vacuum rotary evaporator. The dark brown gummy residues obtained by acid hydrolysis were chromatographed on silica gel column 60×3.2 cm (60–120 mesh, pH 7, Merck) by gradually eluting with n-hexane/ethyl acetate (8 : 2; 6 : 4; 3 : 7 and 1 : 1) and chloroform/methanol (3 : 2). The aliquots of each fraction were subjected to thin layer chromatography (TLC) on silica gel coated TLC plate (1 mm Merck) using the solvent system consisting of 20% (v/v) n-hexane/ethyl acetate. The chromatograms were detected using 50% H2SO4 solution as a spray reagent [34]. The individual fractions were collected and concentrated by vacuum rotary evaporator at 40°C. All the purified compounds recovered from the silica gel column were monitored by reading the absorbance at 190–350 nm (UV spectrophotometer, Hitachi, Japan). The active fractions were further purified, and the final yields of the compounds were recorded. The lyophilized pooled concentrated compounds were then assayed (100 μg/mL) against bacteria. The phytochemical screening was done on the pure compounds using the chemical method previously reported for the detection of secondary metabolites [40]. The different chemical constituents tested include alkaloids, flavonoids, glycosides, polyphenols, saponin, sterols, triterpenes, tannins, reducing sugars, gallic acid, catechol, and aglycones.


### 2.8. Statistical Analysis

The bacterial growth inhibitory activity (inhibition zones millimeter in diameter) was compared for significant differences within the bacterial strains. One way analysis of variance was performed (mean ± SD,n=3 replicates) using GraphPad Prism 4, USA. *P<0.01 was considered statistically significant (inhibition zones of extracts/fractions versus antibiotic drugs).


## 2.1. Ethnomedicinal Survey and Collection of Plants

Ethnomedicinal surveys were conducted during March 1998 and July 2001 from various tribal localities (Kolli hills, Kalrayan hills, Pachamalai, Javadi hills, Mundanthurai) of Eastern and Western Ghats, Tamil Nadu, India. For ethnobotanical studies, questioners were used to collect the general information on the tribes, and the key information on medicinal details was collected through interviews. The medicinal plants were identified by a taxonomist using the standard Flora of Tamil Nadu Carnatic [35], and the voucher specimens were deposited in the department’s herbarium at the Entomology Research Institute, Loyola College, Chennai, India.


## 2.2. Preparation of Plant Extracts

Using a Soxhlet apparatus, the shade-dried and powdered plant materials (200 g of each) were extracted with 1000 mL of methanol (CH3OH) for 10 h. The collected methanol extracts were filtered (Whatman no. 1 filter paper) and evaporated with a rotary evaporator and freeze dryer (lyophilized) to obtain the crude extracts (Buchi, Labortechnik AG, Switzerland). The dried crude extracts were stored at 4°C for antimicrobial assays [34].


## 2.3. Culture of Microorganisms

The following Gram-negative:Burkholderia pseudomallei (TES21), Burkholderia pseudomallei (KHW22), Klebsiella pneumoniae (ATCC15380), Klebsiella pneumoniae, Pseudomonas aeruginosa (ATCC27853), Vibrio damsela, and Salmonella typhi (ATCC51812) and Gram-positive: Staphylococcus aureus (ATCC 29213), Streptococcus pyogenes, and Streptococcus pneumoniae (ATCC49619) microorganisms were used for cultures. B. pseudomallei bacterial strains such as KHW and TES were isolated from the patient samples obtained from the Department of Microbiology, NUS, Singapore. The strains were subcultured on 20 mL Tryptic Soy (TS) and Mueller Hinton (MH) agar plates (pH 7.4) and incubated overnight at 37°C before use.


## 2.4. Antimicrobial Activity

The standard bacterial cultures were stored at −70°C, subcultured on 20 mL MH and TS agar plates (pH 7.4), and incubated overnight at 37°C prior to use. The antimicrobial property was tested using the disc-diffusion method [36]. Five young colonies of each strain of bacteria taken from their respective cultures grown overnight on TS agar plates (Oxoid limited, Wode Road, Basingstoke, Hants, England, UK) were suspended in 5 mL of sterile saline (0.9%), and the density of the suspension was adjusted to approximately 3×108 colony forming unit (CFU). The swab was used to inoculate the dried surface of TS agar plate by streaking four times over the surface of the agar and rotating the plate approximately 90°C to ensure an even distribution of the inoculums. The medium was allowed to dry for about 3 min before adding a 6 millimeter in diameter (mm) sterile paper disc (Becton Dickinson, USA) on the surface. Each disc was tapped gently down onto the agar to provide a uniform contact. Lyophilized residue (100 μg/mL) of each plant extracts and purified fractions was weighed and dissolved in 1 mL of water, and 20 μL of the extracts and oils (containing 100 μg of residue) were applied on each disc (3 replicates), while the sterile blank disc served as a normal control. The antimicrobial effect of the extracts on the clinical isolates was determined in comparison with the reference antibiotics (chloramphenicol 30 μg/disc and ceftazidime 30 μg/disc), which were used as positive controls. The plates were incubated at 37°C for 24 h, and the inhibition zones were measured and calculated.


## 2.5. Minimum Inhibitory Concentrations (MICs) Assay

MICs were evaluated based on thein vitro screening of 16 purified fractions that were found to have potent antimicrobial activity. Broth dilution method was used for the MIC assay with some slight modifications as recommend by the NCCLS [37]. Two-fold serial dilutions of all the fractionated compounds were made with MH and TS broth in microtiter plate wells to adjust the final concentration from 7.8 to 250 μg/mL, while wells containing the broth alone without any sample served as a control. Three replicates (n=3) were used for each dilution and culture containing approximately 1×105 CFU/mL. The plates were incubated at 37°C for 24 h, and the absorbance was measured at 560 nm.


## 2.6. Cytotoxicity Assay

The cytotoxic effects of various extracts were tested by MTT assay [38] using human skin fibroblast HEPK cells. The toxic effect of plant extracts and essential oils was assayed on human skin fibroblast (HEPK) cell proliferation in 96-well microtitre plates. Confluent cells (5×106 cells per well) were incubated with extracts and oils for 24 h, and the percentage inhibitory concentration (IC50) was determined.


## 2.7. Phytochemical Analysis of Plant Extracts

The most active extracts were used for purification of antimicrobial compounds [39]. 100 g each of the plant powder was percolated with 500 mL of 4% aqueous HCl (adjusted to pH 2) and heated at 50°C for 3 h. The extract was washed with 2×250 mL of diethyl ether, and the organic phase evaporated to dryness using vacuum rotary evaporator. The dark brown gummy residues obtained by acid hydrolysis were chromatographed on silica gel column 60×3.2 cm (60–120 mesh, pH 7, Merck) by gradually eluting with n-hexane/ethyl acetate (8 : 2; 6 : 4; 3 : 7 and 1 : 1) and chloroform/methanol (3 : 2). The aliquots of each fraction were subjected to thin layer chromatography (TLC) on silica gel coated TLC plate (1 mm Merck) using the solvent system consisting of 20% (v/v) n-hexane/ethyl acetate. The chromatograms were detected using 50% H2SO4 solution as a spray reagent [34]. The individual fractions were collected and concentrated by vacuum rotary evaporator at 40°C. All the purified compounds recovered from the silica gel column were monitored by reading the absorbance at 190–350 nm (UV spectrophotometer, Hitachi, Japan). The active fractions were further purified, and the final yields of the compounds were recorded. The lyophilized pooled concentrated compounds were then assayed (100 μg/mL) against bacteria. The phytochemical screening was done on the pure compounds using the chemical method previously reported for the detection of secondary metabolites [40]. The different chemical constituents tested include alkaloids, flavonoids, glycosides, polyphenols, saponin, sterols, triterpenes, tannins, reducing sugars, gallic acid, catechol, and aglycones.


## 2.8. Statistical Analysis

The bacterial growth inhibitory activity (inhibition zones millimeter in diameter) was compared for significant differences within the bacterial strains. One way analysis of variance was performed (mean ± SD,n=3 replicates) using GraphPad Prism 4, USA. *P<0.01 was considered statistically significant (inhibition zones of extracts/fractions versus antibiotic drugs).


## 3. Results

### 3.1. Study Area for the Collection of Aromatic Medicinal Plants

The Western and Eastern Ghats were selected for the present study with the cite map showing the landmarks (Figures1(a)-1(b)). Kalrayan hills are situated north of Attur taluk (Salem district), one of the major range of hills in the Eastern Ghats of Tamil Nadu (Figure 1(c)). Pachamalai hills are situated to the north of Thuraiyur taluk of Tiruchirappalli district. The rich biodiversity part of Eastern Ghats lies between latitudes 11°09′ 00′′ to 11° 27′ 00′′ N and longitudes 78°28′ 00′′ to 78° 49′ 00′′ E, and occupies an area of about 527.61 square km. It is located near 11° 11′N 78°21E/11.18°N 78.35°E/11.18; 78.35 (Figure 1(d)). Mundanthurai is located nearly 45 km west of Tirunelveli district, TN, between latitude 8° 25′ and 8° 53′ N and longitude 77° 10′ and 77° 35′ E. This is the only area of Western Ghats that has the longest raining period of about 8 months and forms the catchment area for 14 rivers and streams (Figure 1(e)). Kolli Malai is a small mountain range located in Namakkal district. The mountains are about 1000–1300 m in height and cover an area of approximately 280 km. The Kolli hills are part of the Eastern Ghats, which is a mountain range that runs mostly parallel to the east coast of Tamil Nadu in South India (Figure 1(f)). Javadi hills are one of the largest in the Eastern Ghats in Vellore district in the northern part of the state of Tamil Nadu. They consist of bluish gray hills, with peaks averaging 3600–3800 feet or 1100–1150 meter (Figure 1(g)). Based on the vegetation type (Figures 2(a)–2(d)), the study area consists of (i) dry, deciduous, (ii) moist deciduous, and (iii) rain forests and diverse proportion of plant parts in abundance (Figures 2(e)-2(f)). Three different types of tribes (i.e., Kani, Malayali and Paliyan tribes) inhabit in the hill ranges. The Kani tribes, located at Mundanthurai, raise different types of vegetables in their own fields, while the Malayali tribes cultivate rice. They all engage not only in the agricultural work but also are involved in silvicultural work assigned by the forest department, Government of TN, India.Figure 1
(a) The site for collection of medicinal plants in Western and Eastern Ghats of Tamil Nadu. (b) The landmark (map) of traditional medicine distribution and collection of different types of plants. (c) District map showing the collection site of plants from Kalrayan hills (Salem), (d) Pachamalai hills (Thiruchirappalli), and (e) Mundanthurai (Tirunelveli) rich biodiversity hot-spot of the Western Ghats. (f) Kolli hills (Namakkal), (g) Javadi hills (Vellore), part of the Eastern Ghats, which is a mountain range that runs mostly parallel to the east coast  of South India.Figure 2
Diverse biodiversity richness of medicinal plants in Western and Eastern Ghats. (a) Topography of plant covering area in Kolli hills (Namakkal district, Tamil Nadu). (b) Aerial view of occurrence of medicinal plants in Mundanthurai hills (Tirunelveli district, TN). (c) Pachamalai hills (Trichy district) and its natural vegetation inhabitants for Malaiyali tribes. (d) Deforestation of natural herbal resources due to urbanization in Kalrayan hills (Salem district) in the Eastern Ghats of TN. (e) Medicinal plants and its various parts used by the natives (traditional healers) for the treatment of diverse human illness with a very high percentage of leaves and whole plants often used for herbal drug preparation by the local practitioners. (f) Various category of plants like shrub, herb, climbers and tree, and the  parts used in medicine.


### 3.2. Medicinal Plants Glory

Western Ghats (Mundanthurai) and Eastern Ghats (Kolli hills, Javadi hills, Kalrayan hills, Pachamalai hills) possess a rich diversity of medicinal plants that are used as food and drug by different groups of tribal communities. Urbanization, habitat degradation, and fragmentation of these forests have resulted in the depletion of natural resources on which these tribes used to depend for their livelihoods. It has become increasingly difficult for them to live in their traditional way. In addition, the impact of modernization and urbanization has encroached in and around tribal settlements, thus changing their lifestyles.


### 3.3. Plants Valued as Edibles

Various types of plant parts are collected during different seasons, cooked, and eaten along with boiled rice (Table1). For example, Solanum nigrum leaf is most commonly used in all the four regions. There are a large number of wild edible fruits, including yielding plants such as Citrus acida, Ficus benghalensis, Ficus microcarpa, Ficus racemosa, Phyllanthus emblica, Solanum trilobatum, and Syzygium cuminiare popularly used by the tribes.Table 1
Some of the important traditional medicinal plant species, families, voucher specimens, parts used, yield of extracts, phytochemical screening, and toxicity on human macrophage cells.
Scientific name
Family
Voucher specimen
Plant parts
Yield (gm)
Phytochemical analysis
Adhatoda vasica Nees
Acanthaceae
D2020
Leaf
6.4
Vasicine
Aegle marmelos (L.) Correa ex Roxb.
Rutaceae
D2018
Root-bark
5.8
Alkaloids
Alangium salvifolium (L.) f. Wangerin.
Alangiaceae
0140
Leaf
6.3
Phenolic
Andrographis paniculata Wallich ex Nees
Acanthaceae
0061
Leaf
6.8
Andrographolide
Andrographis echioides Nees
Acanthaceae
0116
Leaf
7.0
Terpenoids,
Acalypha indica L.
Euphorbiaceae
29644
Leaf
6.1
Acalyphe
Acalypha lanceolata L.
Euphorbiaceae
15791
Leaf
7.1
Alkaloids
Achyranthes aspera L.
Amaranthaceae
2666
Leaf
2.7
Betaine
Ageratum conyzoides L.
Asteraceae
4812
Leaf
3.7
Essential oils
Asteracantha longifolia L.
Acanthaceae
0234
Stem
7.4
Glycosides
Azadirachta indica A. Juss.
Meliaceae
D0204
Leaf, bark
6.7
Tannins
Borassus flabellifer L.
Arecaceae
D0202
Root
0.8
Flavonoids, phenolics
Boerhavia erecta L.
Nyctaginaceae
10897
Whole plant
2.8
Phenolics
Calotropis procera (Ait.) Ait. f.
Asclepiadaceae
D073
Root-bark
1.3
Terpenoids
Calotropis gigantea (L.) R.Br.ex Ait
Asclepiadaceae
D070
Milky latex
4.8
Alkaloids
Cassia auriculata L.
Caesalpiniaceae
0141
Leaf
4.9
Saponins
Cassia occidentalis L.
Caesalpiniaceae
0111
Root
9.5
Flavonoids, saponins
Cassia tora L.
Caesalpiniaceae
0100
Stem, bark
6.9
Saponins
Cassia fistula L.
Caesalpiniaceae
037
Whole plant
7.9
Saponins
Cardiospermum halicacabum L.
Sapindaceae
0125
Whole plant
5.8
Flavonoids
Catharanthus roseus (L.) G.Don.
Apocynaceae
0029
Leaf, root
1.40.7
Alkaloids
Cinnamomum zeylanicum Garcin ex Blume
Lauraceae
00209
Bark
3.1
Essential oil, Tannin
Cinnamomum iners Reinw. ex Blume
Lauraceae
043-c
Leaf, bark
Alkaloids
Cissus quadrangularis Roxb.
Vitaceae
D02023
Leaf
6.8
Glycosides
Citrus acida Roxb. Hook.f.
Rutaceae
0213
Leaf
5.2
Saponins, Terpenoids
Centella asiatica (L.)
Umbelliferae
0138
Whole plant
8.9
Flavonoids, Alkaloids
Clerodendrum inerme (L.) Gaertn.
Verbenaceae
D02043
Stem
7.8
Sterols, diterpenes
Clitoria ternatea L.
Papilionaceae
D02026
Seed
9.8
Protein
Cleistanthus collinus
(Roxb.) Benth. and Hook.f.
Euphorbiaceae
0011
Whole plant
0.03
Cleistanthin, collinusin
Cleome gynandropsis L.
Capparidaceae
12247
Leaf
6.2
Glycosides
Cleome viscose L.
Capparidaceae
29999
Leaf
2.7
Phenolics
Coccinia grandis W & A
Cucurbitaceae
D02030
Leaf, root
0.9
Glycosides
Cymbopogon citratus (DC.)
Gramineae
D012
Root
0.25
Essential oil
Datura metel L.
Solanaceae
D02038
Leaf, stem
3.9
Steroids
Eucalyptus globulusLabill.
Myrtaceae
D0220
Leaf
1.2
Terpenoids
Eclipta alba (L.) Hassk
Asteraceae
D028
Whole plant
0.7
Phenolic
Euphorbia hirta Linn
Euphorbiaceae
0018-c
Whole plant
0.12
—
Eclipta prostrata (L.)
Asteraceae
D210
Leaf
1.10
Triterpenoid, saponin
Eugenia caryophyllus(Sprengel) Bullock & Harrison
Myrtaceae
0025
Flower buds
1.16
Essential oils
Elettaria cardamomum White et Mason
Zingiberaceae
0009
Fruit pods
3.17
Essential oils
Gloriosa superba L.
Liliaceae
020-S
Tuber
1.08
Alkaloids, phenol
Jatropha curcas L.
Euphorbiaceae
015
Whole plant
5.3
Alkaloids, flavonoids
Hyptis suaveolens (L.) Poit.
Lamiaceae
24688
Leaf
6.3
Essential oil
Hemidesmus indicus L.
Asclepiadaceae
D-009
Roots
Coumarins
Ichnocarpus  frutescens (L.) R.Br.
Apocynaceae
0110
Root, flower
7.3
Terpenoids
Leucas aspera (Willd.) Link
Labiatae
0114
Leaf
8.3
Triterpenes
Lawsonia inermis L.
Lythraceae
T261
Leaf
0.9
Glycosides, phenolic
Madhuca longifolia (L.) JF Macbr
Sapotaceae
D01415
Nut
9.3
Sitosterol
Merremia hastate L.(Desr.) Hallier.f.
Convolvulaceae
10894
Whole plant
4.0
Alkaloids
Mentha piperita L.
Lamiaceae
0217-c
Whole plant
0.7
Essential oils
Morinda tinctoria Roxb
Rubiaceae
0122
Leaf
1.4
Glycosides
Mimosa pudica L.
Mimosaceae
0071
Whole plant
0.6
—
Oldenlandia umbellata L.
Rubiaceae
D02047
Leaf
4.4
Alkaloids
Ocimum sanctum L.
Lamiaceae
0016
Whole plant
3.0
Alkaloids
Piper attenuatum Buch. Hamex Miq.
Piperaceae
007
Flower
4.6
Alkaloids
Plumbago zeylanica (L.) Cav
Plumbaginaceae
0121
Root
4.8
Plumbagin
Plectranthus amboinicus (L.) Spreng.
Lamiaceae
0410
Whole plant
1.2
Essential oils, terpenoids
Phyllanthus debilisL. (Klein ex Willd)
Euphorbiaceae
0120
Whole plant
4.9
Polyphenol
Phyllanthus madraspatensis L.
Euphorbiaceae
0117
Whole plant
5.0
Polyphenol
Premna tomentosa Willd.
Verbenaceae
0129
Leaf
5.3
Diterpenes
Rosmarinus officinalis L.
Lamiaceae
0017
Root
0.23
Essential oils
Rauwolfia serpentine L.
Apocynaceae
020-S
Root
1.15
Alkaloid
Sebastiania chamaelea (L.) Muell Arg.
Euphorbiaceae
0034
Leaf
1.1
Polyphenol
Solanum trilobatum L.
Solanaceae
D02054
Leaf, flower
4.0
Tannins
Sphaeranthus indicus L.
Asteraceae
D02060
Whole plant
1.0
Essential oil
Swertia chirata (L.) Ham.
Gentianaceae
D0540
Whole plant
1.6
Glycodises
Strychnos nux-vomica L.
Loganiaceae
S-22
Nuts
0.36
Alkaloids
Tragia involucrata L.
Euphorbiaceae
D068
Leaves
1.6
Shellsol
Tinospora cordifolia (Willd.) Miers ex Hoof.f & Thoms
Menispermaceae
0118
Leaf,root, stem
5.0
Glycosides, tannins
Tridax procumbens L.
Compositae
10649
Leaf
1.8
Flavonoids
Terminalia arjuna (DC) W & A
Combretaceae
033-c
Bark
8.0
Phenolics
Tephrosia purpurea (L.) Pers
Fabaceae
S-43
Whole plant
0.8
Isoflavone
Vitex negundo L.
Verbenaceae
0031
Leaf
2.4
Terpineol
Vetiveria zizanioides L.
Gramineae
0051
Root
1.03
Essential oil
Withania somnifera (L.) Dunal
Solanaceae
D02063
Root
2.1
Alkaloids
Wedelia calendulacea Less
Asteraceae
S-24
Leaves
Flavonoids
Zingiber officinale Rosc.
Zingiberaceae
0327
Rhizome
2.3
Tannins
Zanthoxylum limonella (Dennst.) Alston
Rutaceae
009
Bark
1.9
Alkaloids, essential oil
Class of chemical compounds: A: alkaloids, S: saponins, T: tannins, St: steroids, G: glycosides, T: terpenoids, P: polyphenol, P: phenolics, Sh: shellsol, H: hydrocarbon esters.


### 3.4. Plants Used for Snakebite Treatment

Thirty-four plants used for snakebite treatment are documented (Table1). Snakebite is a major health hazard that leads to high mortality in tribal settlements. The majority of the antidotes are prepared freshly from plant materials frequently collected from the leaves of A. paniculata, A. echioides, Aristolochia indica, E. alba, E. prostrata, M. pudica, O. sanctum, T. involucrata, and Cleistanthus collinus (Oduvanthalai); the whole plants of Achyranthes asperaandWedelia calendulacea; the stem-barks and nuts of Strychnos nux-vomica; the roots ofHemidesmus indicus, Tephrosia purpurea, Rauwolfia serpentina,  C. roseus,and so forth, and the tubers of Gloriosa superba. The tuber paste is usually applied externally on the site of snakebite, and decoction is given orally for treatment by indigenous people. Besides, these tribes rely on the medicinal plants as ingredients for fabricating a kind of medicated stone for health management. “Vishakallu” (poison stone) is used by the indigenous groups called Kani in Kerala, India, to treat a snakebite. When the stone is placed directly on the bitten area, it sticks to the body to absorb the poison and then become detached when absorption seems to be complete. The ingredients of Vishakallu stones are made with leaves of Ocimum sanctum, Anisomeles malabarica, Leucas aspera, Piper betle, Santalum album, and the pebbles collected from the river bank.


### 3.5. Survey of Medicinal Plants and Their Health Care Values

The present study is an attempt to provide scientific basis and obtain justification for the traditional beliefs of reliance on a rich diversity of ethnomedicinal plants, along with the rich heritage of traditional medicine practices related to health care system made available by the primitive tribal communities located at different settlements. The native traditional practitioners called “vaidyars” have a good knowledge about the traditional plants locally available for treatment of various diseases (Figures3(a)–3(p)). Such traditional medical knowledge is used for preparing home remedies, ill health prevention, and routine health maintenance. This knowledge is also applicable to cover other sectors of social life. During the ethnobotanical survey, the wealth of 78 medicinal plant species used by the indigenous tribal community for various types of health treatment was documented. The botanical names, family names, parts used, chemical constituents, and their application are provided (Table 1).Figure 3
Medicinal aromatic herbs, spices, and toxic plants were collected from the tribal areas of the Western and Eastern Ghats region in Tamil Nadu, India. (a)A. vasica  Nees (leaf), (b) Eclipta alba (L.) Hassk. (whole plant), (c) Mimosa pudica L. (whole plant), (d) P. amboinicus  (L.)Spreng. (whole plant), (e) T. procumbens  (L.) (whole plant), (f) Euphorbia hirta Linn (whole plant), (g) A. paniculata Wallich ex Nees, (H) C. roseus (L.) G.Don. (whole plant) used for therapy. (i) Cinnamomum inersReinw. ex Blume (leaf) (j) E. globulus Labill. (leaf and bark), (k) Z. officinale Rosc. (Rhizome), (l) E. caryophyllus (Sprengel) Bullock and Harrison(flower buds), (m) M. piperita L. (whole plant), (n) C. citratus(DC.) Clitoria ternatea  L. (whole plant), (o) C. zeylanicum Garcin ex Blume (bark), (p) Elettaria cardamomum White et Mason  (fruit pod) used for medicine and food preparation.


### 3.6. Antimicrobial Activity of Crude Extracts

In this study, we reported the antimicrobial screening of methanolic crude extracts of 78 medicinal plants (Table2). Results revealed that 68 plant extracts displayed potent activities against one or more Gram-positive and -negative bacteria. Of which, Tragia involucrata, Citrus acida, Aegle marmelos, Adhatoda vasica, Calotropis procera, Andrographis paniculata and Mentha piperita, Azadirachta indica, Sphaeranthus indicus, and Elettaria cardamomum showed the highest antibacterial activity against the multidrug resistant B. pseudomallei (KHW and TES) and S. aureus at 100 μg/mL concentration. The extracts showed pronounced antibacterial activity with their inhibitory zones ranging from 20 to 31 mm in diameter as compared to the standard drugs chloramphenicol and ceftazidime (29–33 mm). The majority of the plants demonstrated a powerful antimicrobial potency against the multidrug resistant strains of B. pseudomallei (KHW and TES), K. pneumonia,and S. aureus. Approximately, twenty-one plant extracts exerted only a weak or moderate effect against the tested bacteria, while the crude extract of 13 plants failed to show any effect at all. Except for the plant extracts of T. involucrata, A. lanceolata, A. vasica, and S. indicus extracts, the majority of the plant extracts were ineffective against the V. damselainfection, fascinatingly, only 11 plants exhibited activity against P. aeruginosa,of whichS. indicus, M. piperita,and C. procerawere found to have very strong inhibition of bacteria at the tested concentrations. Interestingly, sixteen plants such as Andrographis echioides, C. auriculata, C. viscose, C. gigantea, T. arjuna, Oldenlandia umbellata, Boerhavia erecta,andE. hirtaexerted a strong activity against the Gram-positive S. aureus  bacteria.Table 2
Antimicrobial activity of methanol extract of aromatic medicinal plants and essential oils evaluated against multidrug resistant (MDR) human pathogens at 100 µg/mL concentration.
Scientific name
Microorganisms; growth inhibition zones (6 millimeter in diameters)
KHW
TES
K.p
K.pr
P.a
S.a
St.p
S.p
V.d
V.d
Adhatoda vasica Nees
28
19
22
18
12
—
10
17
16
—
Aegle marmelos (L.) Correa ex Roxb.
29
17
9
10
—
15
—
—
—
—
Alangium salvifolium (L.) f. Wangerin.
15
10
8
8
—
12
9
10
—
—
Andrographis echioides L.
12
9
—
—
—
17
8
9
—
—
Andrographis paniculata Wallich ex Nees
26
21
19
13
—
25
8
16
12
—
Acalypha indica L.
—
—
—
—
—
18
—
—
—
—
Acalypha lanceolata L.
—
—
12
13
—
10
—
—
21
—
Achyranthes aspera L.
—
—
7
14
—
15
12
11
—
—
Ageratum conyzoides L.
10
8
—
—
—
—
—
—
—
—
Asteracantha longifolia L.
16
12
—
—
—
15
—
—
—
—
Azadirachta indica A. Juss.
15
17
21
16
14
23
12
14
—
—
Borassus flabellifer L.
9
10
17
8
—
—
9
10
—
—
Boerhavia erecta L.
—
—
8
9
7
16
—
—
—
—
Calotropis procera (L.)
—
—
15
—
18
28
9
—
—
—
Calotropis gigantea (L.) R.Br.ex Ait
11
9
—
—
—
20
—
—
9
8
Cardiospermum halicacabum L.
23
—
—
14
—
9
19
—
—
—
Catharanthus roseus (L.) G.Don.
13
7
12
9
—
15
11
8
—
—
Cassia auriculata L.
17
13
12
—
—
19
13
—
—
—
Cassia occidentalis L.
18
—
—
—
—
—
—
—
—
—
Cassia tora L.
—
—
—
—
—
—
—
—
—
—
Cassia fistula L.
—
—
—
—
—
—
—
—
—
—
Citrus acida Roxb. Hook.f.
26
22
—
12
29
9
13
8
—
Cissus quadrangularis L.
—
—
—
—
—
—
—
—
—
—
Cinnamomum zeylanicum Garcin ex Blume
14
16
7
20
—
22
19
7
—
—
Cinnamomum iners Reinw. ex Blume
20
16
—
15
—
16
12
—
—
—
Rosmarinus officinalis L.
—
9
—
10
—
7
—
8
—
—
Centella asiatica (L.)
9
8
—
—
—
11
—
—
—
—
Clerodendrum inerme (L.) Gaertn.
13
7
12
—
—
15
11
8
—
—
Clitoria ternatea L.
—
—
—
—
—
—
—
—
—
—
Clitoria ternatea L.
16
12
—
13
—
8
10
—
—
—
Cleome gynandropsis L.
—
—
12
19
11
—
9
15
12
—
Cleome viscose L.
—
—
8
10
14
20
—
—
—
—
Coccinia grandis W & A
9
10
17
8
—
—
9
10
—
—
Cymbopogon citratus (DC.)
16
18
—
17
—
14
—
—
—
—
Datura metel L.
—
—
—
—
—
—
—
—
—
—
Eclipta alba (L.) Hassk
20
—
—
9
—
—
10
—
—
—
Euphorbia hirta Linn
11
—
—
—
—
16
—
—
—
—
Eucalyptus globulus Labill.
—
—
—
7
—
—
—
7
—
—
Eugenia caryophyllus Bullock & Harrison
—
7
—
11
—
9
—
8
—
—
Elettaria cardamomum White et Mason
21
20
7
14
—
22
12
17
—
—
Hyptis suaveolens (L.) Poit.
8
10
8
7
—
—
—
—
—
—
Ichnocarpus  frutescens (L.) W.J. Aiton
—
—
—
—
—
—
—
—
—
—
Jatropha curcas L.
—
—
—
8
—
11
—
12
—
7
Leucas aspera (Willd.) Link
9
10
17
8
—
12
9
10
—
—
Lawsonia inermis L.
—
—
—
—
—
—
—
—
—
—
Madhuca longifolia (L.) JF Macbr
18
16
14
19
12
—
8
7
9
—
Merremia hastate L. (Desr.) Hallier.f.
—
—
—
—
—
—
—
—
—
—
Morinda tinctoria Roxb
—
—
—
—
—
—
—
—
—
—
Mentha piperita L.
23
17
26
12
20
25
19
—
—
—
Ocimum sanctum L.
12
9
11
7
—
15
7
8
—
—
Oldenlandia umbellata L.
—
—
—
—
—
17
—
—
—
—
Piper attenuatum Buch. Hamex Miq.
13
7
12
21
—
17
11
8
—
—
Plumbago zeylanica (L.) Cav
9
10
17
8
—
12
9
10
—
—
Plectranthus amboinicus (L.) Spreng.
—
—
—
8
11
15
—
—
—
—
Phyllanthus debilis L. (Klein ex Willd)
7
8
—
9
—
9
18
7
—
—
Phyllanthus maderaspatensis L.
17
—
—
—
—
—
—
—
—
—
Premna tomentosa Willd.
13
10
—
15
—
9
10
—
—
—
Gloriosa superba L.
17
16
7
8
—
15
—
—
—
8
Sebastiania chamaelea (L.) Muell Arg.
19
12
—
17
—
13
19
8
—
—
Solanum trilobatum L.
—
—
13
9
—
8
—
—
—
—
Sphaeranthus indicus L.
20
18
21
7
22
—
11
9
—
16
Swertia chirata (L.) Ham.
—
—
—
—
—
—
—
—
—
—
Terminalia arjuna (W. & A)
—
—
—
—
—
16
—
—
—
—
Tinospora cordifolia (Willd.) Miers ex Hoof.f & Thoms
—
—
23
16
—
12
15
—
—
—
Tridax procumbens L.
9
8
7
—
—
14
—
—
—
—
Tragia involucrata L.
25
23
20
—
—
31
28
22
19
—
Vitex negundo L.
—
—
—
—
—
14
—
—
—
—
Vetiveria zizanioides (L.)
11
9
7
7
—
16
—
—
8
Withania somnifera (L.) Dunal
12
20
—
—
—
15
12
—
—
—
Zingiber officinale Rosc.
14
11
7
15
—
7
12
7
—
—
Zanthoxylum limonella (Dennst.) Alston
13
7
12
9
—
—
11
8
—
—
Chloramphenicol (30 µg/disc)
21
12
15
17
29
16
15
18
13
11
Ceftazidime (30 µg/disc)
33
16
22
19
16
25
21
20
12
15
*Bacteria (+/−). Results obtained in the disc diffusion assay; antibacterial activity is expressed as the mean ± SD (n=3), of the inhibition by the extract and its diameter around the discs. One way analysis of variance was performed (mean ± SD, n=3 replicates).  Size of inhibition zones were including the sterile blank discs 6 millimeter (mm) in diameters. Absence of bacterial inhibition indicates (—), antibiotic disc (30 µg/disc).


### 3.7. Phytochemical Screening of Plants

The results obtained from the phytochemical screening as shown in Table1 indicate the presence of various types of secondary metabolites such as polyphenols, tannins, saponins, alkaloids, and glycosides/polysaccharides. Most of the plant extracts relatively rich in alkaloids, phenols, flavonoids, polyphenols, tannins, sterols, and terpenoids were found to inhibit the growth of organisms.


### 3.8. Antimicrobial Activity of Fractioned Compounds

Active components were purified from the most active extracts for further testing. The compound shellsol ofT. involucrataand C. acidaexhibited the most potent action against the antibiotic resistant strains of B. pseudomallei (KHW),S. aureus, B. pseudomallei  (TES), and K. pneumoniae. A. marmelos was also found to inhibit the growth ofB. pseudomallei (KHW) more effectively than other tested bacteria.A. vasica showed the broad spectrum growth inhibitory activity on B. pseudomallei (KHW), K. pneumoniae, K. pneumoniae,  resistant B. pseudomallei (TES), S. pyogenes, and V. damsela.However, E. cardamomumdisplayed antimicrobial activity on some of the B. pseudomallei (KHW), S. pyogenes,  B. pseudomallei(TES), and S. typhi strains. Similarly, A. indica exerted the growth inhibition on K. pneumoniaeand S. aureus.Remarkably, Sebastiania chamaeleawas more active againstK. pneumoniae, K. pneumoniae,and S. pyogenes.The compound from S. indicusinhibited the growth ofK. pneumoniae, K. pneumoniae, B. pseudomallei (KHW and TES), and S. typhistrains, as compared to the activity shown by the crude extracts (Figure 4). The antimicrobial efficacy of fractions collected from the oil yielding plants was also compared with that of the tested compounds. C. zeylanicumand R. officinaliswere the most sensitive in controlling the growth of B. pseudomallei (KHW), S. aureus, K. pneumonia, andS. pneumoniae. Fascinatingly, all the compounds obtained from aromatic plants, except those from E. globules,were found to be very effective against the multidrug resistant human pathogen B. pseudomallei (KHW) that causes melioidosis. On the other hand, compounds from C citrates, O. sanctum, E. caryophyllus, and Z. zizanioide,showed some promising effect only against S. aureus (Figure 5). The activity of the compounds were  pronounced more than that of the oil yielding plant fractions.In vitro antimicrobial activity of purified fractions from the most active plant extracts tested against bacteria. Growth inhibition zones were measured and analyzed with mean ± standard deviation (SD), (n=3) using one way analysis of variance. Level of significance at  (*P>0.01). Most of the fractions exerted a potent inhibitory effect against multidrug resistant Gram-negative bacteria (B. pseudomallei strains KHW and TES), K. pneumonia, and Gram-positive bacteria S. aureus.
(a)
(b)
(c)
(d)
(e)
(f)
(g)
(h)Comparison of antimicrobial effect of plant compounds obtained from the most popularly used sources of essential oils as assayed by the disc-diffusion methodin vitro.  It displayed a powerful activity against B. pseudomallei and S. aureus than the other bacteria strains. Other compounds showed only a moderate or weak action against the tested bacteria. Values for zone of bacterial growth inhibition were presented as mean ± SD, (n=3) with level of significance at (*P>0.01).
(a)
(b)
(c)
(d)
(e)
(f)
(g)
(h)


### 3.9. Minimum Inhibitory Concentrations (MICs)

The antibiotic potential of the purified fractions was obtained from the MIC determination. The hydrocarbon ester shellsol (T. involucrata) and C. acida showed an interesting inhibitory potential against S. aureus(MIC of 7.8 μg/mL) andB. pseudomallei strain of KHW (MIC of 15.6 μg/mL). A. vasica showed an MIC of 15.6 μg/mL against B. pseudomallei (KHW) and an MIC of 31.25 μg/mL against K. pneumoniae, K. pneumoniae, S. pyogenes,and V. damselastrains. Fractions from A. marmelos and terpenoid from A. indicaexerted bacteriostatic effect with MIC values of 31.25 μg/mL on some selected bacteria including B. pseudomallei of KHW,S. aureus,and B. pseudomallei of TES. The MIC of 31.25 μg/mL was found forE. cardamomumagainst S. aureus, K. pneumonia,and S. pyogenes. S. indicusdisplayed a very strong inhibition against MDRK. pneumoniae(MIC of 15.6 μg/mL), and against B. pseudomallei (KHW and TES) at MIC of 31.25 μg/mL. When the antimicrobial efficacies of purified fractions from aromatic plants were compared, the C. zeylanicum fraction displayed an important antimicrobial effect against S. aureus(MIC of 7.8 μg/mL), MDR B. pseudomallei of KHW (MIC of 15.6 μg/mL), and S. pneumoniae(MIC of 31.25 μg/mL). The essential oil from M. piperatea showed MIC value of 31.25 μg/mL against K. pneumoniae, S. aureus, and B. pseudomallei (KHW), respectively. O. sanctumand C. citratusfractions also showed antimicrobial activity (MICs of 31.25–125 μg/mL) only at higher concentrations against the tested bacteria. In addition to that, higher concentrations (>250 μg/mL) (of Vetiveria fractions) were required to inhibit Vibro species, and others (including E. globulus fractions) failed to show any effect at tested concentrations (7.8–125 μg/mL). However, the purified fractions (from most active medicinal plants) showed strong bacteriostatic inhibition against the tested organisms (Table 3).Table 3
Minimum inhibitory concentrations (MICs) of purified plant fractions and essential oils against antibiotic resistant bacteria.
Botanical name
Family
Parts used
Gram-positive and -negative bacteria (MICs µg/mL)
KHW
TES
K.p
K.Pr
S.a
St.p
S.p
V.d
S.t
A. indica Juss.
Meliaceae
Seed (fraction)
31.25
62.5
125
<250
31.25
—
—
—
—
A. marmelos (L.)
Rutaceae
Root-bark (F)
31.25
31.25
—
—
31.25
62.5
125
250
—
A. paniculata Nees
Acanthaceae
Leaf (fraction)
250
—
125
—
62.5
—
—
125
—
A. vasica  Nees
Acanthaceae
Fraction (Stem)
15.6
31.25
31.25
62.5
—
<250
31.25
31.25
—
C. acida Roxb.
Rutaceae
Leaf (fraction)
15.6
62.5
—
—
7.8
—
31.25
—
—
E. cardamomum White et Mason
Acanthaceae
Fraction (WP)
62.5
—
31.25
—
31.25
250
62.5
—
—
S. indicus (L.)
Euphorbiaceae
Whole plant (F)
31.25
125
15.6
—
62.5
—
—
<250
—
T. involucrata (L.)
Euphorbiaceae
Shellosol (leaf)
15.6
31.25
—
—
7.8
62.5
—
—
—
Cinnamomum zeylanicum (L.)
Lauraceae
Bark (fraction)
15.6
62.5
—
—
7.8
31.25
—
—
125
Cymbopogon citratus (L.)
Graminae
Leaf (fraction)
250
<250
62.5
—
62.5
125
—
—
—
Eugenia caryophyllus (L.)
Myrtaceae
Flower buds (F)
62.5
125
—
—
62.5
—
—
—
—
Eucalyptus globulus (L.)
Myrtaceae
Fraction (leaf)
—
—
—
—
—
—
<250
—
<250
Mentha piperita (L.)
Labiatae
Fraction (WP)
—
62.5
31.25
—
31.25
—
<250
—
—
Ocimum sanctum (L.)
Labiatae
Leaf (fraction)
62.5
125
—
31.25
62.5
—
125
<250
—
Rosmarinus officinalis (L.)
Labiatae
Rosemary oil
31.25
—
—
—
31.25
—
—
—
—
Vetiveria zizanioides (L.)
Graminae
Root (fraction)
—
125
125
—
—
—
>250
>250
The bacterial growth inhibitory activity was compared for significant differences within the bacterial strains by broth-dilution method at 250, 125, 62.5, 31.25, 15.6, and 7.8 µg/mL. F: fractions.


### 3.10. Cytotoxic Effects of Plants

When the components were assayed for cytotoxicity against the normal human skin fibroblasts (HEPK) cells, the compounds obtained fromE. cardamomum, T. involucrata, S. indicus, C. acida, A. vasica, A. marmelos, A. indica, and A. paniculata did not show toxicity up to 1000 μg/mL (see Figures S1 and S2 in Supplementary Material available online at  http://dx.doi.org/10.1155/2013/525613). A slight reduction of cell proliferation was noted only at higher doses (2000 μg/mL). In contrast, cell proliferation was markedly reduced after exposure of HEPK cells to O. sanctum, E. globulus, V. zizanioides, C. citratus, and E. globulus compounds. There was no gradual reduction in skin cell proliferation seen after exposure to C. zeylanicum, R. officinalis, and M. piperita (see Figures S3 and S4)  compounds. The toxicity was found to be concentration-dependent when the skin fibroblasts (HEPK) cells were exposed to various compounds. The cell proliferation was increased by the influence of the plant components at the lower concentrations. Whereas the oil yielding plant compounds  showed inhibition of cell proliferation and toxicity at higher doses 250–1000 μg/mL.


## 3.1. Study Area for the Collection of Aromatic Medicinal Plants

The Western and Eastern Ghats were selected for the present study with the cite map showing the landmarks (Figures1(a)-1(b)). Kalrayan hills are situated north of Attur taluk (Salem district), one of the major range of hills in the Eastern Ghats of Tamil Nadu (Figure 1(c)). Pachamalai hills are situated to the north of Thuraiyur taluk of Tiruchirappalli district. The rich biodiversity part of Eastern Ghats lies between latitudes 11°09′ 00′′ to 11° 27′ 00′′ N and longitudes 78°28′ 00′′ to 78° 49′ 00′′ E, and occupies an area of about 527.61 square km. It is located near 11° 11′N 78°21E/11.18°N 78.35°E/11.18; 78.35 (Figure 1(d)). Mundanthurai is located nearly 45 km west of Tirunelveli district, TN, between latitude 8° 25′ and 8° 53′ N and longitude 77° 10′ and 77° 35′ E. This is the only area of Western Ghats that has the longest raining period of about 8 months and forms the catchment area for 14 rivers and streams (Figure 1(e)). Kolli Malai is a small mountain range located in Namakkal district. The mountains are about 1000–1300 m in height and cover an area of approximately 280 km. The Kolli hills are part of the Eastern Ghats, which is a mountain range that runs mostly parallel to the east coast of Tamil Nadu in South India (Figure 1(f)). Javadi hills are one of the largest in the Eastern Ghats in Vellore district in the northern part of the state of Tamil Nadu. They consist of bluish gray hills, with peaks averaging 3600–3800 feet or 1100–1150 meter (Figure 1(g)). Based on the vegetation type (Figures 2(a)–2(d)), the study area consists of (i) dry, deciduous, (ii) moist deciduous, and (iii) rain forests and diverse proportion of plant parts in abundance (Figures 2(e)-2(f)). Three different types of tribes (i.e., Kani, Malayali and Paliyan tribes) inhabit in the hill ranges. The Kani tribes, located at Mundanthurai, raise different types of vegetables in their own fields, while the Malayali tribes cultivate rice. They all engage not only in the agricultural work but also are involved in silvicultural work assigned by the forest department, Government of TN, India.Figure 1
(a) The site for collection of medicinal plants in Western and Eastern Ghats of Tamil Nadu. (b) The landmark (map) of traditional medicine distribution and collection of different types of plants. (c) District map showing the collection site of plants from Kalrayan hills (Salem), (d) Pachamalai hills (Thiruchirappalli), and (e) Mundanthurai (Tirunelveli) rich biodiversity hot-spot of the Western Ghats. (f) Kolli hills (Namakkal), (g) Javadi hills (Vellore), part of the Eastern Ghats, which is a mountain range that runs mostly parallel to the east coast  of South India.Figure 2
Diverse biodiversity richness of medicinal plants in Western and Eastern Ghats. (a) Topography of plant covering area in Kolli hills (Namakkal district, Tamil Nadu). (b) Aerial view of occurrence of medicinal plants in Mundanthurai hills (Tirunelveli district, TN). (c) Pachamalai hills (Trichy district) and its natural vegetation inhabitants for Malaiyali tribes. (d) Deforestation of natural herbal resources due to urbanization in Kalrayan hills (Salem district) in the Eastern Ghats of TN. (e) Medicinal plants and its various parts used by the natives (traditional healers) for the treatment of diverse human illness with a very high percentage of leaves and whole plants often used for herbal drug preparation by the local practitioners. (f) Various category of plants like shrub, herb, climbers and tree, and the  parts used in medicine.


## 3.2. Medicinal Plants Glory

Western Ghats (Mundanthurai) and Eastern Ghats (Kolli hills, Javadi hills, Kalrayan hills, Pachamalai hills) possess a rich diversity of medicinal plants that are used as food and drug by different groups of tribal communities. Urbanization, habitat degradation, and fragmentation of these forests have resulted in the depletion of natural resources on which these tribes used to depend for their livelihoods. It has become increasingly difficult for them to live in their traditional way. In addition, the impact of modernization and urbanization has encroached in and around tribal settlements, thus changing their lifestyles.


## 3.3. Plants Valued as Edibles

Various types of plant parts are collected during different seasons, cooked, and eaten along with boiled rice (Table1). For example, Solanum nigrum leaf is most commonly used in all the four regions. There are a large number of wild edible fruits, including yielding plants such as Citrus acida, Ficus benghalensis, Ficus microcarpa, Ficus racemosa, Phyllanthus emblica, Solanum trilobatum, and Syzygium cuminiare popularly used by the tribes.Table 1
Some of the important traditional medicinal plant species, families, voucher specimens, parts used, yield of extracts, phytochemical screening, and toxicity on human macrophage cells.
Scientific name
Family
Voucher specimen
Plant parts
Yield (gm)
Phytochemical analysis
Adhatoda vasica Nees
Acanthaceae
D2020
Leaf
6.4
Vasicine
Aegle marmelos (L.) Correa ex Roxb.
Rutaceae
D2018
Root-bark
5.8
Alkaloids
Alangium salvifolium (L.) f. Wangerin.
Alangiaceae
0140
Leaf
6.3
Phenolic
Andrographis paniculata Wallich ex Nees
Acanthaceae
0061
Leaf
6.8
Andrographolide
Andrographis echioides Nees
Acanthaceae
0116
Leaf
7.0
Terpenoids,
Acalypha indica L.
Euphorbiaceae
29644
Leaf
6.1
Acalyphe
Acalypha lanceolata L.
Euphorbiaceae
15791
Leaf
7.1
Alkaloids
Achyranthes aspera L.
Amaranthaceae
2666
Leaf
2.7
Betaine
Ageratum conyzoides L.
Asteraceae
4812
Leaf
3.7
Essential oils
Asteracantha longifolia L.
Acanthaceae
0234
Stem
7.4
Glycosides
Azadirachta indica A. Juss.
Meliaceae
D0204
Leaf, bark
6.7
Tannins
Borassus flabellifer L.
Arecaceae
D0202
Root
0.8
Flavonoids, phenolics
Boerhavia erecta L.
Nyctaginaceae
10897
Whole plant
2.8
Phenolics
Calotropis procera (Ait.) Ait. f.
Asclepiadaceae
D073
Root-bark
1.3
Terpenoids
Calotropis gigantea (L.) R.Br.ex Ait
Asclepiadaceae
D070
Milky latex
4.8
Alkaloids
Cassia auriculata L.
Caesalpiniaceae
0141
Leaf
4.9
Saponins
Cassia occidentalis L.
Caesalpiniaceae
0111
Root
9.5
Flavonoids, saponins
Cassia tora L.
Caesalpiniaceae
0100
Stem, bark
6.9
Saponins
Cassia fistula L.
Caesalpiniaceae
037
Whole plant
7.9
Saponins
Cardiospermum halicacabum L.
Sapindaceae
0125
Whole plant
5.8
Flavonoids
Catharanthus roseus (L.) G.Don.
Apocynaceae
0029
Leaf, root
1.40.7
Alkaloids
Cinnamomum zeylanicum Garcin ex Blume
Lauraceae
00209
Bark
3.1
Essential oil, Tannin
Cinnamomum iners Reinw. ex Blume
Lauraceae
043-c
Leaf, bark
Alkaloids
Cissus quadrangularis Roxb.
Vitaceae
D02023
Leaf
6.8
Glycosides
Citrus acida Roxb. Hook.f.
Rutaceae
0213
Leaf
5.2
Saponins, Terpenoids
Centella asiatica (L.)
Umbelliferae
0138
Whole plant
8.9
Flavonoids, Alkaloids
Clerodendrum inerme (L.) Gaertn.
Verbenaceae
D02043
Stem
7.8
Sterols, diterpenes
Clitoria ternatea L.
Papilionaceae
D02026
Seed
9.8
Protein
Cleistanthus collinus
(Roxb.) Benth. and Hook.f.
Euphorbiaceae
0011
Whole plant
0.03
Cleistanthin, collinusin
Cleome gynandropsis L.
Capparidaceae
12247
Leaf
6.2
Glycosides
Cleome viscose L.
Capparidaceae
29999
Leaf
2.7
Phenolics
Coccinia grandis W & A
Cucurbitaceae
D02030
Leaf, root
0.9
Glycosides
Cymbopogon citratus (DC.)
Gramineae
D012
Root
0.25
Essential oil
Datura metel L.
Solanaceae
D02038
Leaf, stem
3.9
Steroids
Eucalyptus globulusLabill.
Myrtaceae
D0220
Leaf
1.2
Terpenoids
Eclipta alba (L.) Hassk
Asteraceae
D028
Whole plant
0.7
Phenolic
Euphorbia hirta Linn
Euphorbiaceae
0018-c
Whole plant
0.12
—
Eclipta prostrata (L.)
Asteraceae
D210
Leaf
1.10
Triterpenoid, saponin
Eugenia caryophyllus(Sprengel) Bullock & Harrison
Myrtaceae
0025
Flower buds
1.16
Essential oils
Elettaria cardamomum White et Mason
Zingiberaceae
0009
Fruit pods
3.17
Essential oils
Gloriosa superba L.
Liliaceae
020-S
Tuber
1.08
Alkaloids, phenol
Jatropha curcas L.
Euphorbiaceae
015
Whole plant
5.3
Alkaloids, flavonoids
Hyptis suaveolens (L.) Poit.
Lamiaceae
24688
Leaf
6.3
Essential oil
Hemidesmus indicus L.
Asclepiadaceae
D-009
Roots
Coumarins
Ichnocarpus  frutescens (L.) R.Br.
Apocynaceae
0110
Root, flower
7.3
Terpenoids
Leucas aspera (Willd.) Link
Labiatae
0114
Leaf
8.3
Triterpenes
Lawsonia inermis L.
Lythraceae
T261
Leaf
0.9
Glycosides, phenolic
Madhuca longifolia (L.) JF Macbr
Sapotaceae
D01415
Nut
9.3
Sitosterol
Merremia hastate L.(Desr.) Hallier.f.
Convolvulaceae
10894
Whole plant
4.0
Alkaloids
Mentha piperita L.
Lamiaceae
0217-c
Whole plant
0.7
Essential oils
Morinda tinctoria Roxb
Rubiaceae
0122
Leaf
1.4
Glycosides
Mimosa pudica L.
Mimosaceae
0071
Whole plant
0.6
—
Oldenlandia umbellata L.
Rubiaceae
D02047
Leaf
4.4
Alkaloids
Ocimum sanctum L.
Lamiaceae
0016
Whole plant
3.0
Alkaloids
Piper attenuatum Buch. Hamex Miq.
Piperaceae
007
Flower
4.6
Alkaloids
Plumbago zeylanica (L.) Cav
Plumbaginaceae
0121
Root
4.8
Plumbagin
Plectranthus amboinicus (L.) Spreng.
Lamiaceae
0410
Whole plant
1.2
Essential oils, terpenoids
Phyllanthus debilisL. (Klein ex Willd)
Euphorbiaceae
0120
Whole plant
4.9
Polyphenol
Phyllanthus madraspatensis L.
Euphorbiaceae
0117
Whole plant
5.0
Polyphenol
Premna tomentosa Willd.
Verbenaceae
0129
Leaf
5.3
Diterpenes
Rosmarinus officinalis L.
Lamiaceae
0017
Root
0.23
Essential oils
Rauwolfia serpentine L.
Apocynaceae
020-S
Root
1.15
Alkaloid
Sebastiania chamaelea (L.) Muell Arg.
Euphorbiaceae
0034
Leaf
1.1
Polyphenol
Solanum trilobatum L.
Solanaceae
D02054
Leaf, flower
4.0
Tannins
Sphaeranthus indicus L.
Asteraceae
D02060
Whole plant
1.0
Essential oil
Swertia chirata (L.) Ham.
Gentianaceae
D0540
Whole plant
1.6
Glycodises
Strychnos nux-vomica L.
Loganiaceae
S-22
Nuts
0.36
Alkaloids
Tragia involucrata L.
Euphorbiaceae
D068
Leaves
1.6
Shellsol
Tinospora cordifolia (Willd.) Miers ex Hoof.f & Thoms
Menispermaceae
0118
Leaf,root, stem
5.0
Glycosides, tannins
Tridax procumbens L.
Compositae
10649
Leaf
1.8
Flavonoids
Terminalia arjuna (DC) W & A
Combretaceae
033-c
Bark
8.0
Phenolics
Tephrosia purpurea (L.) Pers
Fabaceae
S-43
Whole plant
0.8
Isoflavone
Vitex negundo L.
Verbenaceae
0031
Leaf
2.4
Terpineol
Vetiveria zizanioides L.
Gramineae
0051
Root
1.03
Essential oil
Withania somnifera (L.) Dunal
Solanaceae
D02063
Root
2.1
Alkaloids
Wedelia calendulacea Less
Asteraceae
S-24
Leaves
Flavonoids
Zingiber officinale Rosc.
Zingiberaceae
0327
Rhizome
2.3
Tannins
Zanthoxylum limonella (Dennst.) Alston
Rutaceae
009
Bark
1.9
Alkaloids, essential oil
Class of chemical compounds: A: alkaloids, S: saponins, T: tannins, St: steroids, G: glycosides, T: terpenoids, P: polyphenol, P: phenolics, Sh: shellsol, H: hydrocarbon esters.


## 3.4. Plants Used for Snakebite Treatment

Thirty-four plants used for snakebite treatment are documented (Table1). Snakebite is a major health hazard that leads to high mortality in tribal settlements. The majority of the antidotes are prepared freshly from plant materials frequently collected from the leaves of A. paniculata, A. echioides, Aristolochia indica, E. alba, E. prostrata, M. pudica, O. sanctum, T. involucrata, and Cleistanthus collinus (Oduvanthalai); the whole plants of Achyranthes asperaandWedelia calendulacea; the stem-barks and nuts of Strychnos nux-vomica; the roots ofHemidesmus indicus, Tephrosia purpurea, Rauwolfia serpentina,  C. roseus,and so forth, and the tubers of Gloriosa superba. The tuber paste is usually applied externally on the site of snakebite, and decoction is given orally for treatment by indigenous people. Besides, these tribes rely on the medicinal plants as ingredients for fabricating a kind of medicated stone for health management. “Vishakallu” (poison stone) is used by the indigenous groups called Kani in Kerala, India, to treat a snakebite. When the stone is placed directly on the bitten area, it sticks to the body to absorb the poison and then become detached when absorption seems to be complete. The ingredients of Vishakallu stones are made with leaves of Ocimum sanctum, Anisomeles malabarica, Leucas aspera, Piper betle, Santalum album, and the pebbles collected from the river bank.


## 3.5. Survey of Medicinal Plants and Their Health Care Values

The present study is an attempt to provide scientific basis and obtain justification for the traditional beliefs of reliance on a rich diversity of ethnomedicinal plants, along with the rich heritage of traditional medicine practices related to health care system made available by the primitive tribal communities located at different settlements. The native traditional practitioners called “vaidyars” have a good knowledge about the traditional plants locally available for treatment of various diseases (Figures3(a)–3(p)). Such traditional medical knowledge is used for preparing home remedies, ill health prevention, and routine health maintenance. This knowledge is also applicable to cover other sectors of social life. During the ethnobotanical survey, the wealth of 78 medicinal plant species used by the indigenous tribal community for various types of health treatment was documented. The botanical names, family names, parts used, chemical constituents, and their application are provided (Table 1).Figure 3
Medicinal aromatic herbs, spices, and toxic plants were collected from the tribal areas of the Western and Eastern Ghats region in Tamil Nadu, India. (a)A. vasica  Nees (leaf), (b) Eclipta alba (L.) Hassk. (whole plant), (c) Mimosa pudica L. (whole plant), (d) P. amboinicus  (L.)Spreng. (whole plant), (e) T. procumbens  (L.) (whole plant), (f) Euphorbia hirta Linn (whole plant), (g) A. paniculata Wallich ex Nees, (H) C. roseus (L.) G.Don. (whole plant) used for therapy. (i) Cinnamomum inersReinw. ex Blume (leaf) (j) E. globulus Labill. (leaf and bark), (k) Z. officinale Rosc. (Rhizome), (l) E. caryophyllus (Sprengel) Bullock and Harrison(flower buds), (m) M. piperita L. (whole plant), (n) C. citratus(DC.) Clitoria ternatea  L. (whole plant), (o) C. zeylanicum Garcin ex Blume (bark), (p) Elettaria cardamomum White et Mason  (fruit pod) used for medicine and food preparation.


## 3.6. Antimicrobial Activity of Crude Extracts

In this study, we reported the antimicrobial screening of methanolic crude extracts of 78 medicinal plants (Table2). Results revealed that 68 plant extracts displayed potent activities against one or more Gram-positive and -negative bacteria. Of which, Tragia involucrata, Citrus acida, Aegle marmelos, Adhatoda vasica, Calotropis procera, Andrographis paniculata and Mentha piperita, Azadirachta indica, Sphaeranthus indicus, and Elettaria cardamomum showed the highest antibacterial activity against the multidrug resistant B. pseudomallei (KHW and TES) and S. aureus at 100 μg/mL concentration. The extracts showed pronounced antibacterial activity with their inhibitory zones ranging from 20 to 31 mm in diameter as compared to the standard drugs chloramphenicol and ceftazidime (29–33 mm). The majority of the plants demonstrated a powerful antimicrobial potency against the multidrug resistant strains of B. pseudomallei (KHW and TES), K. pneumonia,and S. aureus. Approximately, twenty-one plant extracts exerted only a weak or moderate effect against the tested bacteria, while the crude extract of 13 plants failed to show any effect at all. Except for the plant extracts of T. involucrata, A. lanceolata, A. vasica, and S. indicus extracts, the majority of the plant extracts were ineffective against the V. damselainfection, fascinatingly, only 11 plants exhibited activity against P. aeruginosa,of whichS. indicus, M. piperita,and C. procerawere found to have very strong inhibition of bacteria at the tested concentrations. Interestingly, sixteen plants such as Andrographis echioides, C. auriculata, C. viscose, C. gigantea, T. arjuna, Oldenlandia umbellata, Boerhavia erecta,andE. hirtaexerted a strong activity against the Gram-positive S. aureus  bacteria.Table 2
Antimicrobial activity of methanol extract of aromatic medicinal plants and essential oils evaluated against multidrug resistant (MDR) human pathogens at 100 µg/mL concentration.
Scientific name
Microorganisms; growth inhibition zones (6 millimeter in diameters)
KHW
TES
K.p
K.pr
P.a
S.a
St.p
S.p
V.d
V.d
Adhatoda vasica Nees
28
19
22
18
12
—
10
17
16
—
Aegle marmelos (L.) Correa ex Roxb.
29
17
9
10
—
15
—
—
—
—
Alangium salvifolium (L.) f. Wangerin.
15
10
8
8
—
12
9
10
—
—
Andrographis echioides L.
12
9
—
—
—
17
8
9
—
—
Andrographis paniculata Wallich ex Nees
26
21
19
13
—
25
8
16
12
—
Acalypha indica L.
—
—
—
—
—
18
—
—
—
—
Acalypha lanceolata L.
—
—
12
13
—
10
—
—
21
—
Achyranthes aspera L.
—
—
7
14
—
15
12
11
—
—
Ageratum conyzoides L.
10
8
—
—
—
—
—
—
—
—
Asteracantha longifolia L.
16
12
—
—
—
15
—
—
—
—
Azadirachta indica A. Juss.
15
17
21
16
14
23
12
14
—
—
Borassus flabellifer L.
9
10
17
8
—
—
9
10
—
—
Boerhavia erecta L.
—
—
8
9
7
16
—
—
—
—
Calotropis procera (L.)
—
—
15
—
18
28
9
—
—
—
Calotropis gigantea (L.) R.Br.ex Ait
11
9
—
—
—
20
—
—
9
8
Cardiospermum halicacabum L.
23
—
—
14
—
9
19
—
—
—
Catharanthus roseus (L.) G.Don.
13
7
12
9
—
15
11
8
—
—
Cassia auriculata L.
17
13
12
—
—
19
13
—
—
—
Cassia occidentalis L.
18
—
—
—
—
—
—
—
—
—
Cassia tora L.
—
—
—
—
—
—
—
—
—
—
Cassia fistula L.
—
—
—
—
—
—
—
—
—
—
Citrus acida Roxb. Hook.f.
26
22
—
12
29
9
13
8
—
Cissus quadrangularis L.
—
—
—
—
—
—
—
—
—
—
Cinnamomum zeylanicum Garcin ex Blume
14
16
7
20
—
22
19
7
—
—
Cinnamomum iners Reinw. ex Blume
20
16
—
15
—
16
12
—
—
—
Rosmarinus officinalis L.
—
9
—
10
—
7
—
8
—
—
Centella asiatica (L.)
9
8
—
—
—
11
—
—
—
—
Clerodendrum inerme (L.) Gaertn.
13
7
12
—
—
15
11
8
—
—
Clitoria ternatea L.
—
—
—
—
—
—
—
—
—
—
Clitoria ternatea L.
16
12
—
13
—
8
10
—
—
—
Cleome gynandropsis L.
—
—
12
19
11
—
9
15
12
—
Cleome viscose L.
—
—
8
10
14
20
—
—
—
—
Coccinia grandis W & A
9
10
17
8
—
—
9
10
—
—
Cymbopogon citratus (DC.)
16
18
—
17
—
14
—
—
—
—
Datura metel L.
—
—
—
—
—
—
—
—
—
—
Eclipta alba (L.) Hassk
20
—
—
9
—
—
10
—
—
—
Euphorbia hirta Linn
11
—
—
—
—
16
—
—
—
—
Eucalyptus globulus Labill.
—
—
—
7
—
—
—
7
—
—
Eugenia caryophyllus Bullock & Harrison
—
7
—
11
—
9
—
8
—
—
Elettaria cardamomum White et Mason
21
20
7
14
—
22
12
17
—
—
Hyptis suaveolens (L.) Poit.
8
10
8
7
—
—
—
—
—
—
Ichnocarpus  frutescens (L.) W.J. Aiton
—
—
—
—
—
—
—
—
—
—
Jatropha curcas L.
—
—
—
8
—
11
—
12
—
7
Leucas aspera (Willd.) Link
9
10
17
8
—
12
9
10
—
—
Lawsonia inermis L.
—
—
—
—
—
—
—
—
—
—
Madhuca longifolia (L.) JF Macbr
18
16
14
19
12
—
8
7
9
—
Merremia hastate L. (Desr.) Hallier.f.
—
—
—
—
—
—
—
—
—
—
Morinda tinctoria Roxb
—
—
—
—
—
—
—
—
—
—
Mentha piperita L.
23
17
26
12
20
25
19
—
—
—
Ocimum sanctum L.
12
9
11
7
—
15
7
8
—
—
Oldenlandia umbellata L.
—
—
—
—
—
17
—
—
—
—
Piper attenuatum Buch. Hamex Miq.
13
7
12
21
—
17
11
8
—
—
Plumbago zeylanica (L.) Cav
9
10
17
8
—
12
9
10
—
—
Plectranthus amboinicus (L.) Spreng.
—
—
—
8
11
15
—
—
—
—
Phyllanthus debilis L. (Klein ex Willd)
7
8
—
9
—
9
18
7
—
—
Phyllanthus maderaspatensis L.
17
—
—
—
—
—
—
—
—
—
Premna tomentosa Willd.
13
10
—
15
—
9
10
—
—
—
Gloriosa superba L.
17
16
7
8
—
15
—
—
—
8
Sebastiania chamaelea (L.) Muell Arg.
19
12
—
17
—
13
19
8
—
—
Solanum trilobatum L.
—
—
13
9
—
8
—
—
—
—
Sphaeranthus indicus L.
20
18
21
7
22
—
11
9
—
16
Swertia chirata (L.) Ham.
—
—
—
—
—
—
—
—
—
—
Terminalia arjuna (W. & A)
—
—
—
—
—
16
—
—
—
—
Tinospora cordifolia (Willd.) Miers ex Hoof.f & Thoms
—
—
23
16
—
12
15
—
—
—
Tridax procumbens L.
9
8
7
—
—
14
—
—
—
—
Tragia involucrata L.
25
23
20
—
—
31
28
22
19
—
Vitex negundo L.
—
—
—
—
—
14
—
—
—
—
Vetiveria zizanioides (L.)
11
9
7
7
—
16
—
—
8
Withania somnifera (L.) Dunal
12
20
—
—
—
15
12
—
—
—
Zingiber officinale Rosc.
14
11
7
15
—
7
12
7
—
—
Zanthoxylum limonella (Dennst.) Alston
13
7
12
9
—
—
11
8
—
—
Chloramphenicol (30 µg/disc)
21
12
15
17
29
16
15
18
13
11
Ceftazidime (30 µg/disc)
33
16
22
19
16
25
21
20
12
15
*Bacteria (+/−). Results obtained in the disc diffusion assay; antibacterial activity is expressed as the mean ± SD (n=3), of the inhibition by the extract and its diameter around the discs. One way analysis of variance was performed (mean ± SD, n=3 replicates).  Size of inhibition zones were including the sterile blank discs 6 millimeter (mm) in diameters. Absence of bacterial inhibition indicates (—), antibiotic disc (30 µg/disc).


## 3.7. Phytochemical Screening of Plants

The results obtained from the phytochemical screening as shown in Table1 indicate the presence of various types of secondary metabolites such as polyphenols, tannins, saponins, alkaloids, and glycosides/polysaccharides. Most of the plant extracts relatively rich in alkaloids, phenols, flavonoids, polyphenols, tannins, sterols, and terpenoids were found to inhibit the growth of organisms.


## 3.8. Antimicrobial Activity of Fractioned Compounds

Active components were purified from the most active extracts for further testing. The compound shellsol ofT. involucrataand C. acidaexhibited the most potent action against the antibiotic resistant strains of B. pseudomallei (KHW),S. aureus, B. pseudomallei  (TES), and K. pneumoniae. A. marmelos was also found to inhibit the growth ofB. pseudomallei (KHW) more effectively than other tested bacteria.A. vasica showed the broad spectrum growth inhibitory activity on B. pseudomallei (KHW), K. pneumoniae, K. pneumoniae,  resistant B. pseudomallei (TES), S. pyogenes, and V. damsela.However, E. cardamomumdisplayed antimicrobial activity on some of the B. pseudomallei (KHW), S. pyogenes,  B. pseudomallei(TES), and S. typhi strains. Similarly, A. indica exerted the growth inhibition on K. pneumoniaeand S. aureus.Remarkably, Sebastiania chamaeleawas more active againstK. pneumoniae, K. pneumoniae,and S. pyogenes.The compound from S. indicusinhibited the growth ofK. pneumoniae, K. pneumoniae, B. pseudomallei (KHW and TES), and S. typhistrains, as compared to the activity shown by the crude extracts (Figure 4). The antimicrobial efficacy of fractions collected from the oil yielding plants was also compared with that of the tested compounds. C. zeylanicumand R. officinaliswere the most sensitive in controlling the growth of B. pseudomallei (KHW), S. aureus, K. pneumonia, andS. pneumoniae. Fascinatingly, all the compounds obtained from aromatic plants, except those from E. globules,were found to be very effective against the multidrug resistant human pathogen B. pseudomallei (KHW) that causes melioidosis. On the other hand, compounds from C citrates, O. sanctum, E. caryophyllus, and Z. zizanioide,showed some promising effect only against S. aureus (Figure 5). The activity of the compounds were  pronounced more than that of the oil yielding plant fractions.In vitro antimicrobial activity of purified fractions from the most active plant extracts tested against bacteria. Growth inhibition zones were measured and analyzed with mean ± standard deviation (SD), (n=3) using one way analysis of variance. Level of significance at  (*P>0.01). Most of the fractions exerted a potent inhibitory effect against multidrug resistant Gram-negative bacteria (B. pseudomallei strains KHW and TES), K. pneumonia, and Gram-positive bacteria S. aureus.
(a)
(b)
(c)
(d)
(e)
(f)
(g)
(h)Comparison of antimicrobial effect of plant compounds obtained from the most popularly used sources of essential oils as assayed by the disc-diffusion methodin vitro.  It displayed a powerful activity against B. pseudomallei and S. aureus than the other bacteria strains. Other compounds showed only a moderate or weak action against the tested bacteria. Values for zone of bacterial growth inhibition were presented as mean ± SD, (n=3) with level of significance at (*P>0.01).
(a)
(b)
(c)
(d)
(e)
(f)
(g)
(h)


## 3.9. Minimum Inhibitory Concentrations (MICs)

The antibiotic potential of the purified fractions was obtained from the MIC determination. The hydrocarbon ester shellsol (T. involucrata) and C. acida showed an interesting inhibitory potential against S. aureus(MIC of 7.8 μg/mL) andB. pseudomallei strain of KHW (MIC of 15.6 μg/mL). A. vasica showed an MIC of 15.6 μg/mL against B. pseudomallei (KHW) and an MIC of 31.25 μg/mL against K. pneumoniae, K. pneumoniae, S. pyogenes,and V. damselastrains. Fractions from A. marmelos and terpenoid from A. indicaexerted bacteriostatic effect with MIC values of 31.25 μg/mL on some selected bacteria including B. pseudomallei of KHW,S. aureus,and B. pseudomallei of TES. The MIC of 31.25 μg/mL was found forE. cardamomumagainst S. aureus, K. pneumonia,and S. pyogenes. S. indicusdisplayed a very strong inhibition against MDRK. pneumoniae(MIC of 15.6 μg/mL), and against B. pseudomallei (KHW and TES) at MIC of 31.25 μg/mL. When the antimicrobial efficacies of purified fractions from aromatic plants were compared, the C. zeylanicum fraction displayed an important antimicrobial effect against S. aureus(MIC of 7.8 μg/mL), MDR B. pseudomallei of KHW (MIC of 15.6 μg/mL), and S. pneumoniae(MIC of 31.25 μg/mL). The essential oil from M. piperatea showed MIC value of 31.25 μg/mL against K. pneumoniae, S. aureus, and B. pseudomallei (KHW), respectively. O. sanctumand C. citratusfractions also showed antimicrobial activity (MICs of 31.25–125 μg/mL) only at higher concentrations against the tested bacteria. In addition to that, higher concentrations (>250 μg/mL) (of Vetiveria fractions) were required to inhibit Vibro species, and others (including E. globulus fractions) failed to show any effect at tested concentrations (7.8–125 μg/mL). However, the purified fractions (from most active medicinal plants) showed strong bacteriostatic inhibition against the tested organisms (Table 3).Table 3
Minimum inhibitory concentrations (MICs) of purified plant fractions and essential oils against antibiotic resistant bacteria.
Botanical name
Family
Parts used
Gram-positive and -negative bacteria (MICs µg/mL)
KHW
TES
K.p
K.Pr
S.a
St.p
S.p
V.d
S.t
A. indica Juss.
Meliaceae
Seed (fraction)
31.25
62.5
125
<250
31.25
—
—
—
—
A. marmelos (L.)
Rutaceae
Root-bark (F)
31.25
31.25
—
—
31.25
62.5
125
250
—
A. paniculata Nees
Acanthaceae
Leaf (fraction)
250
—
125
—
62.5
—
—
125
—
A. vasica  Nees
Acanthaceae
Fraction (Stem)
15.6
31.25
31.25
62.5
—
<250
31.25
31.25
—
C. acida Roxb.
Rutaceae
Leaf (fraction)
15.6
62.5
—
—
7.8
—
31.25
—
—
E. cardamomum White et Mason
Acanthaceae
Fraction (WP)
62.5
—
31.25
—
31.25
250
62.5
—
—
S. indicus (L.)
Euphorbiaceae
Whole plant (F)
31.25
125
15.6
—
62.5
—
—
<250
—
T. involucrata (L.)
Euphorbiaceae
Shellosol (leaf)
15.6
31.25
—
—
7.8
62.5
—
—
—
Cinnamomum zeylanicum (L.)
Lauraceae
Bark (fraction)
15.6
62.5
—
—
7.8
31.25
—
—
125
Cymbopogon citratus (L.)
Graminae
Leaf (fraction)
250
<250
62.5
—
62.5
125
—
—
—
Eugenia caryophyllus (L.)
Myrtaceae
Flower buds (F)
62.5
125
—
—
62.5
—
—
—
—
Eucalyptus globulus (L.)
Myrtaceae
Fraction (leaf)
—
—
—
—
—
—
<250
—
<250
Mentha piperita (L.)
Labiatae
Fraction (WP)
—
62.5
31.25
—
31.25
—
<250
—
—
Ocimum sanctum (L.)
Labiatae
Leaf (fraction)
62.5
125
—
31.25
62.5
—
125
<250
—
Rosmarinus officinalis (L.)
Labiatae
Rosemary oil
31.25
—
—
—
31.25
—
—
—
—
Vetiveria zizanioides (L.)
Graminae
Root (fraction)
—
125
125
—
—
—
>250
>250
The bacterial growth inhibitory activity was compared for significant differences within the bacterial strains by broth-dilution method at 250, 125, 62.5, 31.25, 15.6, and 7.8 µg/mL. F: fractions.


## 3.10. Cytotoxic Effects of Plants

When the components were assayed for cytotoxicity against the normal human skin fibroblasts (HEPK) cells, the compounds obtained fromE. cardamomum, T. involucrata, S. indicus, C. acida, A. vasica, A. marmelos, A. indica, and A. paniculata did not show toxicity up to 1000 μg/mL (see Figures S1 and S2 in Supplementary Material available online at  http://dx.doi.org/10.1155/2013/525613). A slight reduction of cell proliferation was noted only at higher doses (2000 μg/mL). In contrast, cell proliferation was markedly reduced after exposure of HEPK cells to O. sanctum, E. globulus, V. zizanioides, C. citratus, and E. globulus compounds. There was no gradual reduction in skin cell proliferation seen after exposure to C. zeylanicum, R. officinalis, and M. piperita (see Figures S3 and S4)  compounds. The toxicity was found to be concentration-dependent when the skin fibroblasts (HEPK) cells were exposed to various compounds. The cell proliferation was increased by the influence of the plant components at the lower concentrations. Whereas the oil yielding plant compounds  showed inhibition of cell proliferation and toxicity at higher doses 250–1000 μg/mL.


## 4. Discussion

The Western Ghats is considered as one of the richest biodiversity hotspots in the world [41]. In this survey, we collected nearly 78 medicinal plants from Western and Eastern Ghats that are edible and popularly used for curing various ailments including snakebite. Traditional remedies have a long-standing history in many tribal settlements in TN, India, and they continue to provide useful and applicable tools for treating ailments [42]. The ingredients that make up the “Vishakallu” stone, which is used as an antidote for snakebite, are different herbs and pebbles available from the river banks. Likewise, aqueous paste and decoction obtained from the leaves of A. paniculata are widely used for snakebite treatment by indigenous people [43]. Previous studies have reported that ethnomedicine plays major roles in conserving the disappearing knowledge of tribal communities [44–47]. The traditional beliefs of reliance on a rich diversity of ethnomedicinal plants located at different settlements have also been confirmed in another study [48, 49]. Herein, we explored the various types of traditional practices reported by the primitive tribal communities with a view to gain further knowledge from such studies.In the present investigation, potentially rich sources of tribal medicine (71 plants) were scientifically evaluated for their antibacterial activity against the MDR bacteria, and the accumulated data was disseminated for the first time to the scientific community. Out of the 71 medicinal plants screened for the antibacterial activity, 10 of them (T. involucrata, C. acida, A. marmelos, A. vasica, C. procera, A. paniculata and M. piperita, A. indica, S. indicus,and E. cardamomum) displayed the highest antibacterial activity against the multidrug resistant B. pseudomallei (KHW and TES) and S. aureus strains. The antibacterial activity of those crude plant extracts was as equally effective as that of the standard drugs. Our findings corroborated with the previous reports made on the antistaphylococcal activity of tribal medicinal plants [34, 50, 51]. On the other hand, isolated components from the most active extracts of T. involucrata, shellsol, and C. acidaexhibited the most potent action against the antibiotic resistant B. pseudomallei (KHW),K. pneumoniae, and S. aureusstrains. These results further confirmed our previous findings on the leaves of T. involucrata and its compounds hydrocarbon ester-like shellsol, which displayed a high antibacterial effect against the different bacterial strains, especially that of S. aureus [34]. Eugenol and caryophyllene are the active agents contained in the M. piperita [52] and O. sanctumplants, which are believed to be mainly responsible for the antimicrobial properties of these plants [53]. Interestingly, the compounds obtained from the aromatic plants such as C. zeylanicumand R. officinalis were also found to be very effective against the multidrug resistant human pathogen K. pneumoniae, S. aureus,S. typhi,and B. pseudomallei (KHW) that causes melioidosis.The inhibitory potential determined for the shellsol (T. involucrata), vaseline (A. vasica), C. acida,andC. zeylanicum  indicates  that  the  MIC of 7.8–31.25 μg/mL found against theB. pseudomallei of KHW, K. pneumoniae, K. pneumoniae, S. pyogenes,andV. damsel,and S. pneumoniaewas quite low.Similarly, lower MIC values were found for M. piperita (MIC of 1.13–2.25 mg/mL) against the above bacterial strains [52]. MIC was found for the most active alcohol extracts of A. salvifolium(MIC 0.034–0.263 mg/mL) on S. aureus [54], and the MIC for S. trilobatumaqueous extracts determined against the tested organisms ranged from 0.06 to 0.5 mg/mL [55]. C. zeylanicum was found to have an effective antibacterial activity (MIC 64 μg/mL) against P. aeruginosa, E. coli, B. subtilis,andS. aureus[56]. Previously, several investigators have demonstrated that active agents exert interesting activity against bacteria even at lower concentrations tested [57, 58]. The bacteriostatic mechanism involves damage to the cell walls of bacteria, followed by inhibition of protein synthesis that ultimately leads to bacterial death [59]. The most active plants are widely used by various tribes as traditional treatment (i.e., cut wounds, skin infection, and scabies), thus indicating the potential for further development into promising drugs. In order to ascertain the safety and efficacy of the most active compounds, their effect on human skin fibroblast cells was evaluated. Chemical constituents of E. cardamomum, T. involucrata, S. indicus, C. acida, A. vasica, A. marmelos, A. indica,and A. paniculata plants failed to produce any noticeable toxicity up to 1000 μg/mL. Although some of the tested compounds exhibited a slight reduction of cell proliferation and some minor morphological changes, such changes were insignificant at lower doses and became evident only at higher doses. However, certain aromatic compounds of O. sanctum, E. globulus, V. zizanioides, C. citratus,andE. globulus plants showed reduction of cell proliferation against HEPK cells.Our phytochemical screening also provides evidence of the presence of several types of compounds that are mainly responsible for the remarkable antibacterial effect of these plants. The differences noted for the bactericidal activity of various plant extracts in this study appears to be directly related to the diversity of compounds (shown in parentheses) that are accumulated in the following plants (e.g.,A. marmelos and O. umbellate) [60–62]. Compounds like tannins, phenol, and polyphenols can bind the Gram-negative bacteria to form a heavy soluble complex on the cell surface, which subsequently disturbs the availability of receptor on cells and kills the bacteria [63]. Several species having wide spectra of antimicrobial activity mainly due to the active constituents such as essential oil, phenolic compounds like thymol, carvacrol in oregano and thyme, eugenol in clove, and cinnamon were also identified previously [64, 65]. Essential oils degrade the cell wall, interact with the cell components, and then disrupt the cytoplasmic membrane [66]. The antimicrobial effect of phenolic compounds may involve multiple modes of action, including damage to the membrane protein, interference with membrane integrated enzymes [67], causing leakage of cellular components, coagulation of cytoplasm, depletion of the proton motive force, alteration of fatty acid and phospholipid constituents, impairment of enzymatic mechanisms for production and metabolism, alteration of nutrient uptake and electron transport [68], influencing the synthesis of DNA and RNA, and destroying protein translation and the function of the mitochondrion in eukaryotes [69]. The mode of action of antimicrobial agents depends on the type of microorganism and is mainly related to their cell wall structure and the outer membrane arrangement. Most of the plant spices and herbs contain complex phenolics (i.e., phenolic acids, flavonoids, tannins, lignans, coumarins, quinines). In addition, the mechanisms of action of each phenolic compound against various bacteria are also very complicated [26, 70]. Further investigation is therefore required to understand the relationship between the antimicrobial action and the chemical structure of every phenolic compound in the tested extracts. The information available from previous pharmacological sources combined with the findings herein reported on the medicinal plant extracts may serve as essential data for future drug development to combat diseases caused by the MDR bacterial strains.



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*Source: 525613-2013-10-08.xml*