# Antioxidant, Antinociceptive, and Anti-Inflammatory Effects of Carotenoids Extracted from Dried Pepper (Capsicum annuum L.)

Authors: Marcela Hernández-Ortega; Alicia Ortiz-Moreno; María Dolores Hernández-Navarro; Germán Chamorro-Cevallos; Lidia Dorantes-Alvarez; Hugo Necoechea-Mondragón
Journal: Journal of Biomedicine and Biotechnology
 (2012)

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## Abstract

Carotenoids extracted from dried peppers were evaluated for their antioxidant, analgesic, and anti-inflammatory activities. Peppers had a substantial carotenoid content: guajillo3406±4 μg/g, pasilla 2933±1 μg/g, and ancho 1437±6 μg/g of sample in dry weight basis. A complex mixture of carotenoids was discovered in each pepper extract. The TLC analysis revealed the presence of chlorophylls in the pigment extract from pasilla and ancho peppers. Guajillo pepper carotenoid extracts exhibited good antioxidant activity and had the best scavenging capacity for the DPPH+ cation (24.2%). They also exhibited significant peripheral analgesic activity at 5, 20, and 80 mg/kg and induced central analgesia at 80 mg/kg. The results suggest that the carotenoids in dried guajillo peppers have significant analgesic and anti-inflammatory benefits and could be useful for pain and inflammation relief.


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## Body

## 1. Introduction

Recently, fruits and vegetables have been recognized as natural sources of various bioactive compounds [1–3]. The main phytochemical compounds present in fruits and vegetables are flavonoids, anthocyanins, vitamins C and E, phenolic compounds, dietary fiber, and carotenoids [4]. A lower risk of developing various chronic diseases has been attributed to these phytochemicals, in particular to their antioxidant and radical-scavenging activities [3]. Because natural antioxidants often exist together in different combinations in nature, researchers have been investigating the additive and synergistic effects of different antioxidants [5, 6].One such vegetable where a variety of antioxidants can be found is the pepper. Pepper belongs to the genusCapsicum, which is comprised of more than 200 varieties, with Capsicum annuum, Capsicum baccatum, Capsicum chinense, Capsicum frutescens, and Capsicum pubescens being the main five species [7, 8]. Peppers are consumed worldwide and their importance has increased gradually to place them among the most consumed spice crops in the world [9]. They are usually consumed as food and used as additives in the food industry. They also have a significant role in traditional medicine. In fact, in Indian, Native American, and Chinese traditional medicine, Capsicum species have been used for the treatment of arthritis, rheumatism, stomach aches, skin rashes, dog/snake bites, and flesh wounds [10, 11]. These therapeutic applications are related to the capsaicinoid, phenolic compound, and carotenoid content of peppers [7, 12].Carotenoids are the pigments responsible for the yellow, orange, and red color of many types of peppers [13]; however, they are more than mere pigments and play an important role as antioxidants as well. In their capacity as antioxidants, carotenoids protect cells and tissues from harmful radical oxygen species (ROS), acting as scavengers of singlet molecular oxygen [14], peroxyl radicals [15, 16], and reactive nitrogen species (RNS) [17].It is a well-established fact that free radicals play an important role in pain [18–21]. Pain is “an unpleasant sensory and emotional experience associated with actual or potential tissue damage” while nociception is defined as “the neural processes of encoding and processing noxious stimuli” [22]. Cervero and Laird have proposed that neurophysiological mechanisms responsible for pain can be divided into at least three stages: (a) brief nociceptive stimuli, (b) chronic pain (i.e., inflammatory processes or tissue damage, and (c) abnormal nociceptive perception, such as allodynia and central pain syndrome [23]. Since pain is the most common symptom for which patients seek medical attention and can affect people of any age, gender, or ethnicity [24, 25], animal models were created to understand the fundamental biology of pain, including the initiation, continuation, and subsequent termination of the painful signal. In addition, animal tests that use electrical, thermal, mechanical, and chemical stimuli were developed to study nociceptive and anti-inflammatory pain [26, 27].Different botanical compounds gained attention as therapeutic agents that relieve pain and inflammation [28–32]. Among these, carotenoids were assayed for their analgesic and anti-inflammatory effects. Researchers discovered that α- and β-carotene enhanced the antinociceptive activity of morphine [33], lycopene reduced the thermal hyperalgesia in diabetic mice [34], and lycopene and astaxanthin reduced the levels of the molecules implicated in the inflammation process [12, 35, 36].Since carotenoids have potential therapeutic significance in pain and inflammation management and peppers are known to contain carotenoids, we wanted to evaluate the carotenoid content of the guajillo, pasilla, and ancho dried peppers. We also aimed to determine the antioxidant activity of the carotenoids extracted from these peppers and evaluate their analgesic and anti-inflammatory effect using the acetic-induced writhing, hot plate, and carrageenan-induced hind paw oedema tests in mice.


## 2. Materials and Methods

### 2.1. Plant Material

The three dried pepper varieties were acquired at a local supermarket in Mexico City (Figure1) The collected plat material was botanically identified and their authenticity was confirmed at the herbarium of the National Medical Center (Centro Medico Nacional) of the Mexican Social Security Institute (Instituto Mexicano del Seguro Social) by MS Abigail Aguilar. The voucher specimens were Guajillo pepper 15660 (Capsicum annuum L.), pasilla pepper 15661 (Capsicum annuum L.), and ancho pepper 15662 (Capsicum annuum L). All of these collections were deposited in the herbarium of the National Medical Center of the Mexican Social Security Institute for future reference.Capsicum annuumL. fruits employed in this study. Guajillo pepper (a), pasilla pepper (b), and ancho pepper (c).
(a)
(b)
(c)For powder preparation, peppers were sliced, deseeded, and cut into 1 cm2 pieces. Pepper pieces were then powdered using a Krups GVX211 grinder and passed through no. 100 mesh sieve (150 micron). The powder obtained from each pepper was kept in air-tight plastic bags and protected from light at 4°C until pigment extraction.


### 2.2. Chemicals

Analytical grade acetone, petroleum ether, ethanol, and hexane were acquired from J. T. Baker (Baker-Mallinckrodt, Mexico), as well as high performance liquid chromatography (HPLC) grade acetonitrile, methanol, tetrahydrofuran, and acetone. DPPH (2,2-diphenyl-1-picrylhydrazyl),β-carotene, β-cryptoxanthin, and violaxanthin were obtained from Sigma-Aldrich (St. Louis, MO, USA).


### 2.3. Pigment Extraction

Extraction of pigments from pepper powder was conducted using the methodology reported previously [37]. Briefly, triplicate samples of 1 g of pepper powder were ground in a mortar with Hyflo Super Cel (diatomaceous earth) and 20 mL of cold acetone (5°C), and then vacuum filtered through a Büchner funnel using no. 4 filter paper that was 10 cm in diameter (Whatman International Ltd., Maidstone, UK). The filtrate was transferred to a separation funnel together with petroleum ether and water. The two layers were allowed to separate without stirring and the lower phase was discarded. The upper ether layer was washed several times with water to eliminate residual acetone. After the water was removed using anhydrous sodium sulfate, the ether phase was transferred to a suitable flask and its volume was adjusted to 10 mL with petroleum ether. The entire procedure was executed under low light conditions in order to prevent carotenoid degradation.


### 2.4. Spectrophotometric Analysis of Carotenoids

Total carotenoid content was obtained spectrophotometrically using a method described previously [38]. This method is based on the chromophore properties of carotenoids which allows for grouping in two isochromic families: yellow (CY) and red (CR). Briefly, the petroleum ether extract obtained as described in Section 2.3 was concentrated under nitrogen steam and then dissolved in 10 mL of acetone. Then, 2 mL mixture was placed in a 3 cm cuvette to measure the absorbance using a UV-Vis spectrophotometer (Genesys 10, Thermo Scientific, USA) at 472 and 508 nm. Absorbencies were recorded to obtain both isochromic carotenoid fractions and total carotenoid (CT) by using the following equations:
(1)CY=((A472×1724.3)-(A508×2450.1))270.9(μg/mL),CR=((A508×2144.0)-(A472×403.3))270.9(μg/mL),CT=CR+CY(μg/mL).


### 2.5. Pigment Identification by Thin-Layer Chromatography

In order to identify the different pigments contained in the extracts obtained from dried peppers, thin-layer chromatography (TLC) was used [39]. For this analysis, pigments were extracted from 2 g pepper powder as described in Section 2.3. The resulting 10 mL petroleum ether extract was concentrated using nitrogen steam. The concentrate was then dissolved in 250 μL n-hexane. The hexane extract (20 μL) was placed on a TLC Silica gel GF254 plate (20 cm × 20 cm, 0.7 mm, Merck, Darmstadt, Germany) and developed in hexane : petroleum ether : acetone (1 : 2 : 1). The TLC analysis was repeated four times.


### 2.6. HPLC Analysis of Pigments

Carotenoid identification in pepper extracts [40] was performed using the Beckman System Gold Liquid Chromatograph HPLC equipment with a model 168 photodiode array detector and the Gold Nouveau V 1.72 Chromatographic Data System (Beckman Coulter, Fullerton, CA, USA). Pigments were extracted from 5 g of guajillo, pasilla, or ancho pepper powder as described in Section 2.3. The resulting ether phase was concentrated using high purity nitrogen steam and the samples were stored in the freezer protected from light until used for analysis. Each concentrated sample was resuspended in 1 mL acetone, filtered through a 0.20 μm pore nylon membrane, and then injected into a Prodigy 5 ODS column (25 cm × 4.6 mm ID, Phenomenex, Torrance, CA, USA) together with an isocratic solvent system of acetonitrile : methanol : tetrahydrofuran (THF) (58 : 35 : 7) pumped at a flow rate of 1 mL/min for the analysis of violaxanthin, β-cryptoxanthin, and β-carotene. Visible detection was achieved at 450 nm. The quantification was performed according to a standard curve prepared with commercial β-carotene, violaxanthin, and β-cryptoxanthin.


### 2.7. DPPH+ (2,2-Diphenyl-1-Picrylhydrazil) Radical Assay

TheDPPH+ radical assay was carried out as described by Liu et al., 2008, with a few modifications [5]. The pigments extracted from the pepper powders were tested for their radical scavenging capacity. To initiate the reaction, 0.5 mL of 0.3 mM DPPH+ solution in ethanol : hexane (1 : 1) was blended with 1 mL pigment extract at a concentration of 100 μg/mL in ethanol : hexane (1 : 1). The mixture was shaken and incubated at room temperature in the dark for 15 min and absorbance was measured at 540 nm with a UV spectrophotometer (Genesys 10 UV, Thermo Scientific, USA). The antioxidant activity of the tested extracts was expressed as
(2)%Inhibition=100-(((Abssample-Absblank)×100)Abscontrol),
where Abssample is the absorbance value of pigment extract plus DPPH+ solution, Absblank is the absorbance value of pigment extract dissolved in ethanol and hexane, and Abscontrol is the absorbance of the DPPH+ solution in ethanol : hexane.


### 2.8. Animals

Ninety 8–10-week-old male CD-1 mice with an average weight of20±5 g were obtained from the Autonomous University of Hidalgo State (Universidad Autónoma del Estado de Hidalgo). Animals were maintained in a room at 22±2°C with 12 h light/dark cycle and free access to food and water in accordance with the Institutional Animal Research Committee guide for the official Mexican standard [41], regarding the production, care, and use of laboratory animals. Twelve hours before each experiment, animals were fasted, receiving only water, in order to avoid food interference with experimental substance absorption. All studies were approved by the Bioethical Committee of the National School of Biological Sciences.


### 2.9. Acute Oral Toxicity Assay

Acute oral toxicity assay for the carotenoid extract from guajillo peppers was conducted according to the guidelines OECD no. 423 [42] of the Organization for Economic Cooperation and Development.


### 2.10. Acetic Acid-Induced Writhing Test

The writhing test was performed following the procedure described previously [43]. Samples were administered to mice intragastrically. Thirty minutes prior to writhing induction, mice were assigned to five different groups of six mice each: group 1 was treated with the vehicle (isotonic saline solution, 0.9%), group 2 with ibuprofen as drug reference (10 mg/kg body weight), and groups 3–5 with the carotenoids extracted from guajillo peppers at a dose of 5, 20, or 80 mg/kg body weight. Writhing was induced by intraperitoneal administration of 0.4% acetic acid. Five minutes after the administration of acetic acid, the number of writhes was counted for 30 min. The percent inhibition of writhing was calculated by using the following formula [44]: (3)%Inhibition=(Meannumberofwrithes(control)-Meannumberofwrithes(test)Meannumberofwrithes(control))×100.


### 2.11. Hot Plate Test

The hot plate test was performed following the method of Asongalem et al. [43]. Pain reflex in response to the thermal stimulus was measured using a Le7406 hot plate (Panlab S2, Cornella, Barcelona, Spain). Thirty minutes prior to the test, mice were placed in five different groups of six mice each and were intragastrically treated in the following manner: group 1 was treated with vehicle (isotonic saline solution, 0.9%), group 2 with indomethacin as reference drug (7 mg/kg body weight), and groups 3–5 with the carotenoids extracted from guajillo peppers at a dose of 5, 20, or 80 mg/kg body weight. Mice were placed on a 55±1°C hot plate in order to obtain their response to electrical heat-induced nociceptive pain stimulus judged by the presence of behaviors such as licking of the fore and hind paws or jumping. The pain response was measured at 30 min and at every hour thereafter for 5 h. The cut-off time used to prevent skin damage was 25 s.


### 2.12. Carrageenan-Induced Mice Paw Oedema

Paw oedema in mice was induced as described previously [45]. Five groups of mice (n=6) were administered intragastrically with vehicle control (isotonic saline solution), indomethacin as a reference drug (7 mg/kg body weight), and carotenoid extract at doses 5, 20, 80 mg/kg body weight, 30 min after carrageenan injection. Acute inflammation was induced by the subplantar administration (in the right paw of the mouse) of 0.1 mL isotonic saline solution containing 3% of carrageenan and Tween 80. The thickness of injected paws was measured before induction of inflammation and at every hour thereafter for 5 h using a 0.01-scale micrometer. The inhibitory activity was calculated according the following formula [46]:
(4)%Inhibition=((Ct-C0)control-(Ct-C0)treated(Ct-C0)control)×100,
where Ct is the paw thickness at timet, C0 is the paw thickness before carrageenan injection, Ct-C0 is thickness of oedema, and (Ct-C0)control is thickness of oedema after carrageenan injection of control mice at time t.


### 2.13. Data Analysis

Carotenoid content data were reported as mean ± standard deviation and analgesic and anti-inflammation data as mean ± standard error of the mean (S.E.M). Statistical analyses were conducted using Statistical Package for the Social Sciences (SPSS), version 15 (SPSS Inc., Chicago, IL, USA). Data were analyzed by oneway analysis of variance (ANOVA) and differences among the means were compared using Tukey’s test for carotenoid content, antioxidant activity, and the writhing test. The Dunnett test was used as a post-hoc test for hot plate and carrageenan assays with a level of significance set atP<0.05.


## 2.1. Plant Material

The three dried pepper varieties were acquired at a local supermarket in Mexico City (Figure1) The collected plat material was botanically identified and their authenticity was confirmed at the herbarium of the National Medical Center (Centro Medico Nacional) of the Mexican Social Security Institute (Instituto Mexicano del Seguro Social) by MS Abigail Aguilar. The voucher specimens were Guajillo pepper 15660 (Capsicum annuum L.), pasilla pepper 15661 (Capsicum annuum L.), and ancho pepper 15662 (Capsicum annuum L). All of these collections were deposited in the herbarium of the National Medical Center of the Mexican Social Security Institute for future reference.Capsicum annuumL. fruits employed in this study. Guajillo pepper (a), pasilla pepper (b), and ancho pepper (c).
(a)
(b)
(c)For powder preparation, peppers were sliced, deseeded, and cut into 1 cm2 pieces. Pepper pieces were then powdered using a Krups GVX211 grinder and passed through no. 100 mesh sieve (150 micron). The powder obtained from each pepper was kept in air-tight plastic bags and protected from light at 4°C until pigment extraction.


## 2.2. Chemicals

Analytical grade acetone, petroleum ether, ethanol, and hexane were acquired from J. T. Baker (Baker-Mallinckrodt, Mexico), as well as high performance liquid chromatography (HPLC) grade acetonitrile, methanol, tetrahydrofuran, and acetone. DPPH (2,2-diphenyl-1-picrylhydrazyl),β-carotene, β-cryptoxanthin, and violaxanthin were obtained from Sigma-Aldrich (St. Louis, MO, USA).


## 2.3. Pigment Extraction

Extraction of pigments from pepper powder was conducted using the methodology reported previously [37]. Briefly, triplicate samples of 1 g of pepper powder were ground in a mortar with Hyflo Super Cel (diatomaceous earth) and 20 mL of cold acetone (5°C), and then vacuum filtered through a Büchner funnel using no. 4 filter paper that was 10 cm in diameter (Whatman International Ltd., Maidstone, UK). The filtrate was transferred to a separation funnel together with petroleum ether and water. The two layers were allowed to separate without stirring and the lower phase was discarded. The upper ether layer was washed several times with water to eliminate residual acetone. After the water was removed using anhydrous sodium sulfate, the ether phase was transferred to a suitable flask and its volume was adjusted to 10 mL with petroleum ether. The entire procedure was executed under low light conditions in order to prevent carotenoid degradation.


## 2.4. Spectrophotometric Analysis of Carotenoids

Total carotenoid content was obtained spectrophotometrically using a method described previously [38]. This method is based on the chromophore properties of carotenoids which allows for grouping in two isochromic families: yellow (CY) and red (CR). Briefly, the petroleum ether extract obtained as described in Section 2.3 was concentrated under nitrogen steam and then dissolved in 10 mL of acetone. Then, 2 mL mixture was placed in a 3 cm cuvette to measure the absorbance using a UV-Vis spectrophotometer (Genesys 10, Thermo Scientific, USA) at 472 and 508 nm. Absorbencies were recorded to obtain both isochromic carotenoid fractions and total carotenoid (CT) by using the following equations:
(1)CY=((A472×1724.3)-(A508×2450.1))270.9(μg/mL),CR=((A508×2144.0)-(A472×403.3))270.9(μg/mL),CT=CR+CY(μg/mL).


## 2.5. Pigment Identification by Thin-Layer Chromatography

In order to identify the different pigments contained in the extracts obtained from dried peppers, thin-layer chromatography (TLC) was used [39]. For this analysis, pigments were extracted from 2 g pepper powder as described in Section 2.3. The resulting 10 mL petroleum ether extract was concentrated using nitrogen steam. The concentrate was then dissolved in 250 μL n-hexane. The hexane extract (20 μL) was placed on a TLC Silica gel GF254 plate (20 cm × 20 cm, 0.7 mm, Merck, Darmstadt, Germany) and developed in hexane : petroleum ether : acetone (1 : 2 : 1). The TLC analysis was repeated four times.


## 2.6. HPLC Analysis of Pigments

Carotenoid identification in pepper extracts [40] was performed using the Beckman System Gold Liquid Chromatograph HPLC equipment with a model 168 photodiode array detector and the Gold Nouveau V 1.72 Chromatographic Data System (Beckman Coulter, Fullerton, CA, USA). Pigments were extracted from 5 g of guajillo, pasilla, or ancho pepper powder as described in Section 2.3. The resulting ether phase was concentrated using high purity nitrogen steam and the samples were stored in the freezer protected from light until used for analysis. Each concentrated sample was resuspended in 1 mL acetone, filtered through a 0.20 μm pore nylon membrane, and then injected into a Prodigy 5 ODS column (25 cm × 4.6 mm ID, Phenomenex, Torrance, CA, USA) together with an isocratic solvent system of acetonitrile : methanol : tetrahydrofuran (THF) (58 : 35 : 7) pumped at a flow rate of 1 mL/min for the analysis of violaxanthin, β-cryptoxanthin, and β-carotene. Visible detection was achieved at 450 nm. The quantification was performed according to a standard curve prepared with commercial β-carotene, violaxanthin, and β-cryptoxanthin.


## 2.7. DPPH+ (2,2-Diphenyl-1-Picrylhydrazil) Radical Assay

TheDPPH+ radical assay was carried out as described by Liu et al., 2008, with a few modifications [5]. The pigments extracted from the pepper powders were tested for their radical scavenging capacity. To initiate the reaction, 0.5 mL of 0.3 mM DPPH+ solution in ethanol : hexane (1 : 1) was blended with 1 mL pigment extract at a concentration of 100 μg/mL in ethanol : hexane (1 : 1). The mixture was shaken and incubated at room temperature in the dark for 15 min and absorbance was measured at 540 nm with a UV spectrophotometer (Genesys 10 UV, Thermo Scientific, USA). The antioxidant activity of the tested extracts was expressed as
(2)%Inhibition=100-(((Abssample-Absblank)×100)Abscontrol),
where Abssample is the absorbance value of pigment extract plus DPPH+ solution, Absblank is the absorbance value of pigment extract dissolved in ethanol and hexane, and Abscontrol is the absorbance of the DPPH+ solution in ethanol : hexane.


## 2.8. Animals

Ninety 8–10-week-old male CD-1 mice with an average weight of20±5 g were obtained from the Autonomous University of Hidalgo State (Universidad Autónoma del Estado de Hidalgo). Animals were maintained in a room at 22±2°C with 12 h light/dark cycle and free access to food and water in accordance with the Institutional Animal Research Committee guide for the official Mexican standard [41], regarding the production, care, and use of laboratory animals. Twelve hours before each experiment, animals were fasted, receiving only water, in order to avoid food interference with experimental substance absorption. All studies were approved by the Bioethical Committee of the National School of Biological Sciences.


## 2.9. Acute Oral Toxicity Assay

Acute oral toxicity assay for the carotenoid extract from guajillo peppers was conducted according to the guidelines OECD no. 423 [42] of the Organization for Economic Cooperation and Development.


## 2.10. Acetic Acid-Induced Writhing Test

The writhing test was performed following the procedure described previously [43]. Samples were administered to mice intragastrically. Thirty minutes prior to writhing induction, mice were assigned to five different groups of six mice each: group 1 was treated with the vehicle (isotonic saline solution, 0.9%), group 2 with ibuprofen as drug reference (10 mg/kg body weight), and groups 3–5 with the carotenoids extracted from guajillo peppers at a dose of 5, 20, or 80 mg/kg body weight. Writhing was induced by intraperitoneal administration of 0.4% acetic acid. Five minutes after the administration of acetic acid, the number of writhes was counted for 30 min. The percent inhibition of writhing was calculated by using the following formula [44]: (3)%Inhibition=(Meannumberofwrithes(control)-Meannumberofwrithes(test)Meannumberofwrithes(control))×100.


## 2.11. Hot Plate Test

The hot plate test was performed following the method of Asongalem et al. [43]. Pain reflex in response to the thermal stimulus was measured using a Le7406 hot plate (Panlab S2, Cornella, Barcelona, Spain). Thirty minutes prior to the test, mice were placed in five different groups of six mice each and were intragastrically treated in the following manner: group 1 was treated with vehicle (isotonic saline solution, 0.9%), group 2 with indomethacin as reference drug (7 mg/kg body weight), and groups 3–5 with the carotenoids extracted from guajillo peppers at a dose of 5, 20, or 80 mg/kg body weight. Mice were placed on a 55±1°C hot plate in order to obtain their response to electrical heat-induced nociceptive pain stimulus judged by the presence of behaviors such as licking of the fore and hind paws or jumping. The pain response was measured at 30 min and at every hour thereafter for 5 h. The cut-off time used to prevent skin damage was 25 s.


## 2.12. Carrageenan-Induced Mice Paw Oedema

Paw oedema in mice was induced as described previously [45]. Five groups of mice (n=6) were administered intragastrically with vehicle control (isotonic saline solution), indomethacin as a reference drug (7 mg/kg body weight), and carotenoid extract at doses 5, 20, 80 mg/kg body weight, 30 min after carrageenan injection. Acute inflammation was induced by the subplantar administration (in the right paw of the mouse) of 0.1 mL isotonic saline solution containing 3% of carrageenan and Tween 80. The thickness of injected paws was measured before induction of inflammation and at every hour thereafter for 5 h using a 0.01-scale micrometer. The inhibitory activity was calculated according the following formula [46]:
(4)%Inhibition=((Ct-C0)control-(Ct-C0)treated(Ct-C0)control)×100,
where Ct is the paw thickness at timet, C0 is the paw thickness before carrageenan injection, Ct-C0 is thickness of oedema, and (Ct-C0)control is thickness of oedema after carrageenan injection of control mice at time t.


## 2.13. Data Analysis

Carotenoid content data were reported as mean ± standard deviation and analgesic and anti-inflammation data as mean ± standard error of the mean (S.E.M). Statistical analyses were conducted using Statistical Package for the Social Sciences (SPSS), version 15 (SPSS Inc., Chicago, IL, USA). Data were analyzed by oneway analysis of variance (ANOVA) and differences among the means were compared using Tukey’s test for carotenoid content, antioxidant activity, and the writhing test. The Dunnett test was used as a post-hoc test for hot plate and carrageenan assays with a level of significance set atP<0.05.


## 3. Results and Discussion

### 3.1. Guajillo, Pasilla, and Ancho Peppers Have High and Differing Levels of Carotenoids

In order to determine the antioxidant, analgesic, and anti-inflammatory benefits of the guajillo, pasilla, and ancho peppers, we first measured their carotenoid content. A significant difference was observed (P<0.05) in total carotenoid content among the three peppers analyzed (Table 1). Guajillo peppers had the highest carotenoid content followed by pasilla and ancho peppers (3406±4, 2933±1, and 1437±6 μg/g in dry weight basis (DWB), resp.). Previously, researchers had reported a total carotenoid content of 3721 μg/g in guajillo pepper, 6226 μg/g in pasilla pepper, and 3070 μg/g in ancho pepper [47]. Recently, another study reported guajillo pepper’s carotenoid content as 876 μg/g DWB [48]. These differences in carotenoid content among several reports could be due to the differences in genotype, maturity stage, and drying process [47]. According to [49], red paprika pepper has a carotenoid content of 2354 μg/g DWB, which is an amount higher than that of a carrot (1824 μg/g DWB) and almost equal to that of a tomato (2047 μg/g DWB). Therefore, despite the varying carotenoid contents reported in the literature, there is mounting evidence to suggest that peppers are also a good source of carotenoids.Table 1
Isochromic fractions (yellow and red) and total carotenoid contents of extracts from dried peppers (μg/g).
Peppers
Total carotenoid content (CT)
Yellow isochromic fraction (CY)
Red isochromic fraction (CR)
Guajillo
3406
±
4
a
1501
±
8
a
1905
±
12
a
Pasilla
2933
±
1
b
1225
±
7
b
1708
±
6
b
Ancho
1437
±
6
c
543
±
4
c
894
±
10
c
Results expressed as mean ± standard deviation,n=3. Values followed by different letters (a, b, c) within a column are significantly different (one-way ANOVA, Tukey’s post-hoc test, P≤0.05).Guajillo peppers contained the highest yellow fraction at1501±8 μg/g (Table 1). According to [38], the yellow fraction comprised bands with Rf (reference front) from 0.96 to 0.69, including β-carotene, esterified β-cryptoxanthin, violaxanthin, antheraxanthin, cucurbitaxanthin, and esterified zeaxanthin, whereas the red fraction comprised bands with Rf from 0.37 to 0.20 and included esterified capsanthin and capsorubin. Several groups have demonstrated that the yellow fraction of carotenoids exhibits significant antioxidant activity [50–52].The pigment extracts from the three peppers analyzed by TLC and HPLC had a complex mixture of carotenoids (Tables2 and 3). Interestingly, TLC analysis revealed that ancho and pasilla pepper extracts contained green bands associated with chlorophyll content (Figure 2). The carotenoid profile of guajillo peppers identified by TLC was in agreement with a previous report by Zeb and Murkovic [39].Table 2
R
f of pigments from guajillo, pasilla, and ancho peppers separated by TLC.
Carotenoids
Band color
Guajillo
Pasilla
Ancho
R
f
R
f
R
f
β-Carotene
Yellow
0.98
0.98
0.98
Capsanthin
Red
0.91
0.91
0.91
Violaxanthin
Yellow
0.80
0.80
0.80
β-Cryptoxanthin
Yellow
0.60
0.60
0.60
Zeaxanthin
Orange
0.56
0.56
0.56
Lutein epoxide
Yellow
0.47
0.47
0.47
Capsorubin
Yellow
0.34
0.34
0.34
Neoxanthin
Orange
0.22
0.22
0.22Table 3
Carotenoid contents in ancho, guajillo, and pasilla peppers determined by HPLC analysis.
Carotenoid
Retention time (min)
Guajillo pepper
Pasillapepper
Anchopepper
Violaxanthin
4.8
1671.0 ± 0.05a
3667.2 ± 0.02b
746.2 ± 0.05c
β-Cryptoxanthin
10.7
407.2 ± 0.05a
212.5 ± 0.05b
185.6 ± 0.01c
β-Carotene
36.5
344.0 ± 0.05a
47.7 ± 0.07b
220.4 ± 0.05c
Results are expressed inμg/g DWB (mean ± SD, n=3). Values followed by different letters (a, b, c) in the same row are significantly different (P≤0.05, one way ANOVA, Tukey’s post-hoc test).Figure 2
TLC profile of pigments in dried peppers. (a) Ancho pepper, (b) guajillo pepper, (c) pasilla pepper. Ancho and pasilla pepper extracts have green bands, revealing the presence of chlorophylls in these peppers.We found that the contents of violaxanthin,β-carotene, and β-cryptoxanthin were significantly different (P<0.05) among the three peppers studied (Table 3). Guajillo pepper had the highest β-carotene and β-cryptoxanthin content, and pasilla pepper contained the highest amount of violaxanthin. Wall et al. (2001) reported that pasilla peppers contain 539±63 μg/g β-carotene, guajillo peppers, 343±54 μg/g, and ancho peppers, 317±41 μg/g. On the other hand, Hervert-Hernández et al. (2010) reported that guajillo peppers contain 73 μg/g β-carotene and only 20 μg/g β-cryptoxanthin. Differences among the reported carotenoid contents of these peppers may be due to the differences in genotype, maturity stage, and drying process [47, 48].Since the aim of the study was to evaluate the antioxidant, analgesic, and anti-inflammatory activity of carotenoids, ancho and pasilla pepper extracts were not used in further analyses of analgesia and anti-inflammatory properties due to the presence of chlorophylls in their extracts.


### 3.2. Guajillo, Pasilla, and Ancho Peppers Have Significantly Different Antioxidant Capacities

We next determined the antioxidant capacity of guajillo, pasilla, and ancho peppers by using theDPPH+ scavenging assay. Our results demonstrated that guajillo extracts had a 24.2% scavenging capacity, which was significantly higher than that of pasilla peppers (15.6%) and ancho peppers (12.3%, P<0.05). The differences in the antioxidant activity can be attributed to the higher β-carotene and β-cryptoxanthin contents of guajillo peppers, since these carotenoids are known to exert significant antioxidant activity [6, 53, 54].Researchers have proposed that the types of carotenoids, their concentration, and the ratio at which they are mixed are important variables affecting the antioxidant capacity of an extract [52, 55, 56]. Recently Müller et al. observed that sea buckthorn berry juice had the most complex mixture of carotenoids (lutein, zeaxanthin, β-cryptoxanthin, β-carotene, α-carotene, and lycopene), which correlated to its high antioxidant activity [6]. In addition, Zanfini et al. reported that the cherry tomato, which had a higher carotenoid content than other tomato varieties, generated the highest antioxidant activity in a DPPH+ assay [55]. The function of dietary carotenoids and their metabolites in the human body clearly depends on a wide range of factors beyond the basic chemical properties of these molecules per se [52]; however, our results indicate that the carotenoid extracts from guajillo peppers have high antioxidant activity.


### 3.3. Guajillo Pepper Extract Is Not Toxic Even at High Doses

Guajillo pepper carotenoid extract did not cause any mortality, morbidity symptoms, or other deleterious effects even at the highest dose tested (2000 mg/kg, intragastrical administration), which indicated a reasonable margin of safety.


### 3.4. Guajillo Extract Significantly Reduced Acetic Acid-Induced Writhing in Mice

Both the carotenoid extract from guajillo pepper administrated at 5, 20, and 80 mg/kg and 10 mg/kg ibuprofen significantly reduced the number of writhing episodes induced by acetic acid compared to the control treatment (P<0.05, Table 4). The percentage of writhing inhibition was 62.0% for ibuprofen and 56.4, 47.9, and 43.9% for carotenoid extracts at 5, 20, and 80 mg/kg, respectively.Table 4
The effect of pigments extracted from guajillo pepper on acetic acid-induced writhing test in mice.
Treatment
Dose (mg/kg)
Number of writhes per 30 min
Inhibition (%)
Control
0
71 ± 2
—
Ibuprofen
10
27 ± 9a
62
Carotenoidsextracted fromguajillo pepper
5
34 ± 6a
56
20
37 ± 10a
48
80
40 ± 5a
44
Results are expressed as mean ± S.E.M (n=6). Pa≤0.05, statistical significance with respect to control was calculated by one-way ANOVA followed by Tukey’s test. Isotonic saline solution was used as the control and ibuprofen as the reference treatment.Zhu et al. (2011) also reported a similar percentage of inhibition of writhing in mice using 100 and 200 mg/kg ethanol extract ofDesmodium podocarpum, which is a type of shrub used in Chinese folk medicine. Similarly, Huang et al. (2011) reported 50% writhing inhibition in mice treated with 1000 mg/kg of aqueous extract of Xanthii  fructus, which is a Chinese seed used in traditional medicine. In addition, Roome et al. (2011) obtained 64% writhing inhibition with 100 mg/kg ethyl acetate extract and 84% with 10 mg/kg methanol extract from the stems of Aegiceras corniculatum, which is a mangrove plant used in traditional medicine [57–59]. The results of these studies as well as ours suggest that different substances present in seeds, shrubs, and stems of selected plants have analgesic activities similar to the carotenoids extracted with petroleum ether from guajillo peppers.The acetic acid writhing test is often used to test the nonselective antinociceptive effects of a substance. When animals are intraperitoneally injected with acetic acid, a painful reaction together with acute inflammation emerges in the peritoneal area. The resulting stimulation of peritoneal nociceptors is indirectly caused by the release of endogenous factors, such as algogen, bradykinins, and prostanoids, which stimulate nerve ends [60, 61]. The analgesic effect of the guajillo pepper carotenoid extract may be due to inhibition of the local prostaglandins. However, further assays are necessary to conclude that the antinociceptive effect of guajillo peppers is due to peripheral analgesia.


### 3.5. Guajillo Pepper Extract Prolongs Tactile Heat Tolerance of Mice

The results of the analgesic effect of the carotenoid extract of guajillo pepper assayed by a hot plate test are shown in Table5. Guajillo pepper carotenoid extract at the highest dose tested, 80 mg/kg, as well as indomethacin (7 mg/kg), significantly increased the latency of response compared to the control group (P<0.05). Interestingly, guajillo pepper carotenoid extract had a more prolonged effect than indomethacin, increasing the latency of response time even after 5 h posttreatment.Table 5
Antinociceptive effect of the carotenoid extract from guajillo pepper using a hot plate test on mice.
Treatment
Dose (mg/kg)
Latency of response time (s)
Before treatment
After treatment
0 min
30 min
60 min
120 min
180 min
240 min
300 min
360 min
Control
0
5 ± 0.4
7 ± 0.4
8 ± 1
7 ± 1
9 ± 1
8 ± 1
9 ± 1
8 ± 1
Indomethacin
7
5 ± 1
12 ± 2a
12 ± 1a
12 ± 1a
14 ± 2a
13 ± 1a
13 ± 2
12 ± 2
Carotenoids extractedfrom guajillo pepper
5
5 ± 0.2
9 ± 1
9 ± 1
8 ± 1
11 ± 2
10 ± 2
12 ± 1
10 ± 1
20
5 ± 1
6 ± 1
7 ± 1
10 ± 1
9 ± 1
11 ± 1
10 ± 1
12 ± 3
80
5 ± 1
9 ± 2
10 ± 2a
15 ± 4a
15 ± 3a
15 ± 3a
18 ± 3a
18 ± 4a
Results are expressed as mean ± S.E.M (n=6) of response time (s). aStatistical significance with respect to the control was calculated by one-way ANOVA followed by Dunnett’s test (P≤0.05). Isotonic saline solution was used as the control and indomethacin was used as the reference treatment.The hot plate test is usually used in conjunction with the writhing test to distinguish central nervous system effects from peripheral ones [62]. Our results indicated that 80 mg/kg guajillo pepper carotenoid extract significantly prolonged the time that mice could endure heat stimuli, suggesting that the antinociceptive effect of the extract was mainly due to the participation of the central nervous system.There are several recent studies that have demonstrated that pigment extracts from plants and shrubs can exhibit analgesic effects. For example, Mothana et al. (2012) found that 500 mg/kg methanol extract ofLoranthus regularis Steud ex Sprague(a hemiparasitic shrub widely distributed in Yemen and Southern Saudi Arabia) presented a central analgesic effect, which was nearly comparable with that of indomethacin. More recently, Taïwe et al. (2012) reported that a dose of 160 mg/kg aqueous Crassocephalum bauchiense(Hutch, Asteraceae family, leaves used in traditional medicine in Cameroon) extract caused a marked increase in the latency response in mice during hot plate algesiometer tests for nociception. Similarly, Zhu et al. (2011) reported that the petroleum ether fraction from the ethanol extract of Desmodium podocarpumat a dose of 200 mg/kg significantly prolonged the latency period of mice compared to the group given distilled water. Moreover, Roome et al. (2011) demonstrated that the ethyl acetate extract from the stems of Aegiceras corniculatum at doses of 10, 25, and 50 mg/kg caused significant antinociceptive effects. Furthermore, Ashok Kumar et al. (2010) reported that methanol extracts of Amaranthus viridis (A. viridis), A. caudatus, andA. spinosusshowed significant pain management effects in albino mice at a dose of 400 mg/kg (P<0.01), which was attributed to the presence of alkaloids, steroids, flavonoids, terpenoids, and phenolic compounds in these extracts [30, 57, 58, 63, 64].In fact, the antinociceptive effect of many plants has been attributed to their flavonoid [65–67], triterpene [68, 69], or tannin, anthocyanin, alkaloid, and saponin content [70]. Our results suggest that we can add carotenoids of guajillo peppers to the growing list of compounds with antinociceptive activity.


### 3.6. Anti-Inflammatory Effect of Carotenoid Extract

The guajillo pepper carotenoid extract significantly inhibited oedema formation and progression at a dose of 5 mg/kg compared to the control treatment at 1, 3, and 5 hours after carrageenan injection (P<0.05, Table 6). A similar response was obtained with indomethacin compared to the control treatment. Interestingly, at higher doses (20 and 80 mg/kg), the guajillo pepper extract significantly reduced oedema generated by the carrageenan at the 5 h time point (P<0.05).Table 6
The effect of carotenoids extracted from dried guajillo pepper on carrageenan-induced paw oedema.
Treatment
Dose (mg/kg)
Initial paw size (mm)
Paw oedema (mm)
1 h
3 h
5 h
Control
0
3.0 ± 0.1
1.5 ± 0.1
1.7 ± 0.1
1.9 ± 0.8
Indomethacin
7
3.0 ± 0.1
0.9 ± 0.2a
1.1 ± 0.2a
0.8 ± 0.2a
Carotenoids extractedfrom guajillo pepper
5
2.9 ± 0.1
1.1 ± 0.2a
1.1 ± 0.1a
0.9 ± 0.1a
20
2.9 ± 0.1
1.1 ± 0.1
1.6 ± 0.1
1.1 ± 0.1a
80
3.0 ± 0.2
1.2 ± 0.1
1.3 ± 0.1
1.0 ± 0.1a
Results are expressed as mean ± S.E.M (n=6). aStatistical significance with respect to the control treatment was calculated by one-way ANOVA followed by the Dunnett’s test (P≤0.05). Isotonic saline solution was used as the control and indomethacin was used as the reference treatment.The carrageenan-induced paw oedema test is suitable for evaluation of anti-inflammatory drugs and assessment of the antioedematous effects of natural products [71]. There are two phases of carrageenan-induced inflammatory reaction: early phase (from 0 to 2.5 h after injection of carrageenan), which results from the activity of serotonin and bradykinins as well as histamine liberation, and late phase, which is associated with the release of prostaglandins [72].A large number of studies have indicated that analgesic and anti-inflammatory activities of herbs may be attributed to their natural phenolic components, such as caffeic acid and caffeoylquinic acid, which have antioxidant activities [73]. In fact, Zhu et al. (2011) found that the anti-inflammatory activity of Desmodium podocarpumethanol extract was related to the presence of five major families of compounds in this shrub: phenols, phytosterols, arylpropionic acids, saponins, and enols. In addition, Mothana et al. (2012) concluded that the anti-inflammatory effect of the Loranthus regularis Steud ex Spraguemethanol extract was associated with its flavonoid content. Along the same line, Spiller et al. (2008) and Loganayaki et al. (2012) reported that flavonoids and phenolic constituents of Ammannia bacciferaL. (a weed that grows in the rice fields in India) exhibited significant anti-inflammatory and analgesic benefits at oral doses of 100 and 200 mg/kg. Importantly, the analgesic effect of the higher dose of this extract (200 mg/kg) was comparable to standard painkillers, including aspirin and morphine. Moreover, Zimmer et al. (2012) suggested that the anti-inflammatory activity of Capsicum baccatum(a red pepper, the most consumed spice in Brazil) may be induced by capsaicin. Furthermore, Hosseinzadeh and Younesi (2002) reported that Crocus sativusL (Iridaceae, commonly known as saffron, used in traditional medicine) extracts have acute and/or chronic anti-inflammatory activity, which may be due to their content of flavonoids, tannins, anthocyanins, alkaloids, and saponins [7, 28, 57, 63, 70, 74].In summary, many plant compounds have anti-inflammatory effects. Our results indicate that carotenoids present in the petroleum ether extract from guajillo peppers clearly belong to the group of compounds with significant anti-inflammatory activity.


## 3.1. Guajillo, Pasilla, and Ancho Peppers Have High and Differing Levels of Carotenoids

In order to determine the antioxidant, analgesic, and anti-inflammatory benefits of the guajillo, pasilla, and ancho peppers, we first measured their carotenoid content. A significant difference was observed (P<0.05) in total carotenoid content among the three peppers analyzed (Table 1). Guajillo peppers had the highest carotenoid content followed by pasilla and ancho peppers (3406±4, 2933±1, and 1437±6 μg/g in dry weight basis (DWB), resp.). Previously, researchers had reported a total carotenoid content of 3721 μg/g in guajillo pepper, 6226 μg/g in pasilla pepper, and 3070 μg/g in ancho pepper [47]. Recently, another study reported guajillo pepper’s carotenoid content as 876 μg/g DWB [48]. These differences in carotenoid content among several reports could be due to the differences in genotype, maturity stage, and drying process [47]. According to [49], red paprika pepper has a carotenoid content of 2354 μg/g DWB, which is an amount higher than that of a carrot (1824 μg/g DWB) and almost equal to that of a tomato (2047 μg/g DWB). Therefore, despite the varying carotenoid contents reported in the literature, there is mounting evidence to suggest that peppers are also a good source of carotenoids.Table 1
Isochromic fractions (yellow and red) and total carotenoid contents of extracts from dried peppers (μg/g).
Peppers
Total carotenoid content (CT)
Yellow isochromic fraction (CY)
Red isochromic fraction (CR)
Guajillo
3406
±
4
a
1501
±
8
a
1905
±
12
a
Pasilla
2933
±
1
b
1225
±
7
b
1708
±
6
b
Ancho
1437
±
6
c
543
±
4
c
894
±
10
c
Results expressed as mean ± standard deviation,n=3. Values followed by different letters (a, b, c) within a column are significantly different (one-way ANOVA, Tukey’s post-hoc test, P≤0.05).Guajillo peppers contained the highest yellow fraction at1501±8 μg/g (Table 1). According to [38], the yellow fraction comprised bands with Rf (reference front) from 0.96 to 0.69, including β-carotene, esterified β-cryptoxanthin, violaxanthin, antheraxanthin, cucurbitaxanthin, and esterified zeaxanthin, whereas the red fraction comprised bands with Rf from 0.37 to 0.20 and included esterified capsanthin and capsorubin. Several groups have demonstrated that the yellow fraction of carotenoids exhibits significant antioxidant activity [50–52].The pigment extracts from the three peppers analyzed by TLC and HPLC had a complex mixture of carotenoids (Tables2 and 3). Interestingly, TLC analysis revealed that ancho and pasilla pepper extracts contained green bands associated with chlorophyll content (Figure 2). The carotenoid profile of guajillo peppers identified by TLC was in agreement with a previous report by Zeb and Murkovic [39].Table 2
R
f of pigments from guajillo, pasilla, and ancho peppers separated by TLC.
Carotenoids
Band color
Guajillo
Pasilla
Ancho
R
f
R
f
R
f
β-Carotene
Yellow
0.98
0.98
0.98
Capsanthin
Red
0.91
0.91
0.91
Violaxanthin
Yellow
0.80
0.80
0.80
β-Cryptoxanthin
Yellow
0.60
0.60
0.60
Zeaxanthin
Orange
0.56
0.56
0.56
Lutein epoxide
Yellow
0.47
0.47
0.47
Capsorubin
Yellow
0.34
0.34
0.34
Neoxanthin
Orange
0.22
0.22
0.22Table 3
Carotenoid contents in ancho, guajillo, and pasilla peppers determined by HPLC analysis.
Carotenoid
Retention time (min)
Guajillo pepper
Pasillapepper
Anchopepper
Violaxanthin
4.8
1671.0 ± 0.05a
3667.2 ± 0.02b
746.2 ± 0.05c
β-Cryptoxanthin
10.7
407.2 ± 0.05a
212.5 ± 0.05b
185.6 ± 0.01c
β-Carotene
36.5
344.0 ± 0.05a
47.7 ± 0.07b
220.4 ± 0.05c
Results are expressed inμg/g DWB (mean ± SD, n=3). Values followed by different letters (a, b, c) in the same row are significantly different (P≤0.05, one way ANOVA, Tukey’s post-hoc test).Figure 2
TLC profile of pigments in dried peppers. (a) Ancho pepper, (b) guajillo pepper, (c) pasilla pepper. Ancho and pasilla pepper extracts have green bands, revealing the presence of chlorophylls in these peppers.We found that the contents of violaxanthin,β-carotene, and β-cryptoxanthin were significantly different (P<0.05) among the three peppers studied (Table 3). Guajillo pepper had the highest β-carotene and β-cryptoxanthin content, and pasilla pepper contained the highest amount of violaxanthin. Wall et al. (2001) reported that pasilla peppers contain 539±63 μg/g β-carotene, guajillo peppers, 343±54 μg/g, and ancho peppers, 317±41 μg/g. On the other hand, Hervert-Hernández et al. (2010) reported that guajillo peppers contain 73 μg/g β-carotene and only 20 μg/g β-cryptoxanthin. Differences among the reported carotenoid contents of these peppers may be due to the differences in genotype, maturity stage, and drying process [47, 48].Since the aim of the study was to evaluate the antioxidant, analgesic, and anti-inflammatory activity of carotenoids, ancho and pasilla pepper extracts were not used in further analyses of analgesia and anti-inflammatory properties due to the presence of chlorophylls in their extracts.


## 3.2. Guajillo, Pasilla, and Ancho Peppers Have Significantly Different Antioxidant Capacities

We next determined the antioxidant capacity of guajillo, pasilla, and ancho peppers by using theDPPH+ scavenging assay. Our results demonstrated that guajillo extracts had a 24.2% scavenging capacity, which was significantly higher than that of pasilla peppers (15.6%) and ancho peppers (12.3%, P<0.05). The differences in the antioxidant activity can be attributed to the higher β-carotene and β-cryptoxanthin contents of guajillo peppers, since these carotenoids are known to exert significant antioxidant activity [6, 53, 54].Researchers have proposed that the types of carotenoids, their concentration, and the ratio at which they are mixed are important variables affecting the antioxidant capacity of an extract [52, 55, 56]. Recently Müller et al. observed that sea buckthorn berry juice had the most complex mixture of carotenoids (lutein, zeaxanthin, β-cryptoxanthin, β-carotene, α-carotene, and lycopene), which correlated to its high antioxidant activity [6]. In addition, Zanfini et al. reported that the cherry tomato, which had a higher carotenoid content than other tomato varieties, generated the highest antioxidant activity in a DPPH+ assay [55]. The function of dietary carotenoids and their metabolites in the human body clearly depends on a wide range of factors beyond the basic chemical properties of these molecules per se [52]; however, our results indicate that the carotenoid extracts from guajillo peppers have high antioxidant activity.


## 3.3. Guajillo Pepper Extract Is Not Toxic Even at High Doses

Guajillo pepper carotenoid extract did not cause any mortality, morbidity symptoms, or other deleterious effects even at the highest dose tested (2000 mg/kg, intragastrical administration), which indicated a reasonable margin of safety.


## 3.4. Guajillo Extract Significantly Reduced Acetic Acid-Induced Writhing in Mice

Both the carotenoid extract from guajillo pepper administrated at 5, 20, and 80 mg/kg and 10 mg/kg ibuprofen significantly reduced the number of writhing episodes induced by acetic acid compared to the control treatment (P<0.05, Table 4). The percentage of writhing inhibition was 62.0% for ibuprofen and 56.4, 47.9, and 43.9% for carotenoid extracts at 5, 20, and 80 mg/kg, respectively.Table 4
The effect of pigments extracted from guajillo pepper on acetic acid-induced writhing test in mice.
Treatment
Dose (mg/kg)
Number of writhes per 30 min
Inhibition (%)
Control
0
71 ± 2
—
Ibuprofen
10
27 ± 9a
62
Carotenoidsextracted fromguajillo pepper
5
34 ± 6a
56
20
37 ± 10a
48
80
40 ± 5a
44
Results are expressed as mean ± S.E.M (n=6). Pa≤0.05, statistical significance with respect to control was calculated by one-way ANOVA followed by Tukey’s test. Isotonic saline solution was used as the control and ibuprofen as the reference treatment.Zhu et al. (2011) also reported a similar percentage of inhibition of writhing in mice using 100 and 200 mg/kg ethanol extract ofDesmodium podocarpum, which is a type of shrub used in Chinese folk medicine. Similarly, Huang et al. (2011) reported 50% writhing inhibition in mice treated with 1000 mg/kg of aqueous extract of Xanthii  fructus, which is a Chinese seed used in traditional medicine. In addition, Roome et al. (2011) obtained 64% writhing inhibition with 100 mg/kg ethyl acetate extract and 84% with 10 mg/kg methanol extract from the stems of Aegiceras corniculatum, which is a mangrove plant used in traditional medicine [57–59]. The results of these studies as well as ours suggest that different substances present in seeds, shrubs, and stems of selected plants have analgesic activities similar to the carotenoids extracted with petroleum ether from guajillo peppers.The acetic acid writhing test is often used to test the nonselective antinociceptive effects of a substance. When animals are intraperitoneally injected with acetic acid, a painful reaction together with acute inflammation emerges in the peritoneal area. The resulting stimulation of peritoneal nociceptors is indirectly caused by the release of endogenous factors, such as algogen, bradykinins, and prostanoids, which stimulate nerve ends [60, 61]. The analgesic effect of the guajillo pepper carotenoid extract may be due to inhibition of the local prostaglandins. However, further assays are necessary to conclude that the antinociceptive effect of guajillo peppers is due to peripheral analgesia.


## 3.5. Guajillo Pepper Extract Prolongs Tactile Heat Tolerance of Mice

The results of the analgesic effect of the carotenoid extract of guajillo pepper assayed by a hot plate test are shown in Table5. Guajillo pepper carotenoid extract at the highest dose tested, 80 mg/kg, as well as indomethacin (7 mg/kg), significantly increased the latency of response compared to the control group (P<0.05). Interestingly, guajillo pepper carotenoid extract had a more prolonged effect than indomethacin, increasing the latency of response time even after 5 h posttreatment.Table 5
Antinociceptive effect of the carotenoid extract from guajillo pepper using a hot plate test on mice.
Treatment
Dose (mg/kg)
Latency of response time (s)
Before treatment
After treatment
0 min
30 min
60 min
120 min
180 min
240 min
300 min
360 min
Control
0
5 ± 0.4
7 ± 0.4
8 ± 1
7 ± 1
9 ± 1
8 ± 1
9 ± 1
8 ± 1
Indomethacin
7
5 ± 1
12 ± 2a
12 ± 1a
12 ± 1a
14 ± 2a
13 ± 1a
13 ± 2
12 ± 2
Carotenoids extractedfrom guajillo pepper
5
5 ± 0.2
9 ± 1
9 ± 1
8 ± 1
11 ± 2
10 ± 2
12 ± 1
10 ± 1
20
5 ± 1
6 ± 1
7 ± 1
10 ± 1
9 ± 1
11 ± 1
10 ± 1
12 ± 3
80
5 ± 1
9 ± 2
10 ± 2a
15 ± 4a
15 ± 3a
15 ± 3a
18 ± 3a
18 ± 4a
Results are expressed as mean ± S.E.M (n=6) of response time (s). aStatistical significance with respect to the control was calculated by one-way ANOVA followed by Dunnett’s test (P≤0.05). Isotonic saline solution was used as the control and indomethacin was used as the reference treatment.The hot plate test is usually used in conjunction with the writhing test to distinguish central nervous system effects from peripheral ones [62]. Our results indicated that 80 mg/kg guajillo pepper carotenoid extract significantly prolonged the time that mice could endure heat stimuli, suggesting that the antinociceptive effect of the extract was mainly due to the participation of the central nervous system.There are several recent studies that have demonstrated that pigment extracts from plants and shrubs can exhibit analgesic effects. For example, Mothana et al. (2012) found that 500 mg/kg methanol extract ofLoranthus regularis Steud ex Sprague(a hemiparasitic shrub widely distributed in Yemen and Southern Saudi Arabia) presented a central analgesic effect, which was nearly comparable with that of indomethacin. More recently, Taïwe et al. (2012) reported that a dose of 160 mg/kg aqueous Crassocephalum bauchiense(Hutch, Asteraceae family, leaves used in traditional medicine in Cameroon) extract caused a marked increase in the latency response in mice during hot plate algesiometer tests for nociception. Similarly, Zhu et al. (2011) reported that the petroleum ether fraction from the ethanol extract of Desmodium podocarpumat a dose of 200 mg/kg significantly prolonged the latency period of mice compared to the group given distilled water. Moreover, Roome et al. (2011) demonstrated that the ethyl acetate extract from the stems of Aegiceras corniculatum at doses of 10, 25, and 50 mg/kg caused significant antinociceptive effects. Furthermore, Ashok Kumar et al. (2010) reported that methanol extracts of Amaranthus viridis (A. viridis), A. caudatus, andA. spinosusshowed significant pain management effects in albino mice at a dose of 400 mg/kg (P<0.01), which was attributed to the presence of alkaloids, steroids, flavonoids, terpenoids, and phenolic compounds in these extracts [30, 57, 58, 63, 64].In fact, the antinociceptive effect of many plants has been attributed to their flavonoid [65–67], triterpene [68, 69], or tannin, anthocyanin, alkaloid, and saponin content [70]. Our results suggest that we can add carotenoids of guajillo peppers to the growing list of compounds with antinociceptive activity.


## 3.6. Anti-Inflammatory Effect of Carotenoid Extract

The guajillo pepper carotenoid extract significantly inhibited oedema formation and progression at a dose of 5 mg/kg compared to the control treatment at 1, 3, and 5 hours after carrageenan injection (P<0.05, Table 6). A similar response was obtained with indomethacin compared to the control treatment. Interestingly, at higher doses (20 and 80 mg/kg), the guajillo pepper extract significantly reduced oedema generated by the carrageenan at the 5 h time point (P<0.05).Table 6
The effect of carotenoids extracted from dried guajillo pepper on carrageenan-induced paw oedema.
Treatment
Dose (mg/kg)
Initial paw size (mm)
Paw oedema (mm)
1 h
3 h
5 h
Control
0
3.0 ± 0.1
1.5 ± 0.1
1.7 ± 0.1
1.9 ± 0.8
Indomethacin
7
3.0 ± 0.1
0.9 ± 0.2a
1.1 ± 0.2a
0.8 ± 0.2a
Carotenoids extractedfrom guajillo pepper
5
2.9 ± 0.1
1.1 ± 0.2a
1.1 ± 0.1a
0.9 ± 0.1a
20
2.9 ± 0.1
1.1 ± 0.1
1.6 ± 0.1
1.1 ± 0.1a
80
3.0 ± 0.2
1.2 ± 0.1
1.3 ± 0.1
1.0 ± 0.1a
Results are expressed as mean ± S.E.M (n=6). aStatistical significance with respect to the control treatment was calculated by one-way ANOVA followed by the Dunnett’s test (P≤0.05). Isotonic saline solution was used as the control and indomethacin was used as the reference treatment.The carrageenan-induced paw oedema test is suitable for evaluation of anti-inflammatory drugs and assessment of the antioedematous effects of natural products [71]. There are two phases of carrageenan-induced inflammatory reaction: early phase (from 0 to 2.5 h after injection of carrageenan), which results from the activity of serotonin and bradykinins as well as histamine liberation, and late phase, which is associated with the release of prostaglandins [72].A large number of studies have indicated that analgesic and anti-inflammatory activities of herbs may be attributed to their natural phenolic components, such as caffeic acid and caffeoylquinic acid, which have antioxidant activities [73]. In fact, Zhu et al. (2011) found that the anti-inflammatory activity of Desmodium podocarpumethanol extract was related to the presence of five major families of compounds in this shrub: phenols, phytosterols, arylpropionic acids, saponins, and enols. In addition, Mothana et al. (2012) concluded that the anti-inflammatory effect of the Loranthus regularis Steud ex Spraguemethanol extract was associated with its flavonoid content. Along the same line, Spiller et al. (2008) and Loganayaki et al. (2012) reported that flavonoids and phenolic constituents of Ammannia bacciferaL. (a weed that grows in the rice fields in India) exhibited significant anti-inflammatory and analgesic benefits at oral doses of 100 and 200 mg/kg. Importantly, the analgesic effect of the higher dose of this extract (200 mg/kg) was comparable to standard painkillers, including aspirin and morphine. Moreover, Zimmer et al. (2012) suggested that the anti-inflammatory activity of Capsicum baccatum(a red pepper, the most consumed spice in Brazil) may be induced by capsaicin. Furthermore, Hosseinzadeh and Younesi (2002) reported that Crocus sativusL (Iridaceae, commonly known as saffron, used in traditional medicine) extracts have acute and/or chronic anti-inflammatory activity, which may be due to their content of flavonoids, tannins, anthocyanins, alkaloids, and saponins [7, 28, 57, 63, 70, 74].In summary, many plant compounds have anti-inflammatory effects. Our results indicate that carotenoids present in the petroleum ether extract from guajillo peppers clearly belong to the group of compounds with significant anti-inflammatory activity.


## 4. Conclusions

The three peppers evaluated in this study are good sources of carotenoids. The carotenoid extract obtained from guajillo peppers exhibited high antioxidant activity, safe margin of toxicity, and significant analgesic and anti-inflammatory benefits. Although our study revealed some possible and novel benefits of the guajillo pepper, future studies are necessary to thoroughly assess the safety of the guajillo carotenoid extract. In addition, further research is necessary to explore the exact antinociceptive and anti-inflammatory mechanism(s) of the guajillo carotenoid extract.



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*Source: 524019-2012-10-02.xml*