# Antifungal Activity of New Eugenol-Benzoxazole Hybrids againstCandida spp.

Authors: Larissa Incerti Santos de Carvalho; Dalila Junqueira Alvarenga; Letícia Cruz Ferreira do Carmo; Lucas Gomes de Oliveira; Naiara Chaves Silva; Amanda Latércia Tranches Dias; Luiz Felipe Leomil Coelho; Thiago Belarmino de Souza; Danielle Ferreira Dias; Diogo Teixeira Carvalho
Journal: Journal of Chemistry
 (2017)

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## Abstract

Eugenol is a natural allylphenol responsible for a wide range of biological activities, especially antimicrobial. Benzoxazoles are heterocycles with recognized antimicrobial activities. This paper describes the design, synthesis, and the biological results for benzoxazole type derivatives of eugenol as antifungal agents. The products were obtained in good yields by a four-step synthetic sequence involving aromatic nitration, nitroreduction, amide formation, and cycle condensation. They were evaluated against species ofCandida spp. in microdilution assays, and four products (5a, 5b′, 5c, and 5d′) were about five times more active than eugenol againstC. albicans and C. glabrata. Two of them (5b′ and 5d′) showed good activity againstC. krusei, a species which is naturally resistant to fluconazole. Furthermore, the active products were more selective than eugenol against human blood cells, showing that they are interesting substances for further optimization.


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## Body

## 1. Introduction

Infectious diseases are among the leading causes of deaths annually, mainly in low income countries [1]. In this context, opportunistic fungal infections, for example, candidiasis, represent a serious medical problem, since they may affect immunocompromised patients.Candida spp. infections are usually controlled by azoles or amphotericin B-based therapies, but the increasing fungal resistance findings limit the therapeutic success and new drugs have been constantly sought [2].Heterocyclic compounds are widely known for their distinct biological activities and many of them constitute pharmacophores in important drug molecules as macrolides, aminoglycosides andβ-lactam antibiotics, azole antifungals, HIV protease and integrase inhibitors, and natural anticancer agents [3]. Nitrogen-based heterocycles are among the most frequently used ring systems in drug discovery [4]. Benzoxazoles are nitrogen-oxygen containing heterocycles that occur in a number of natural products and are easily obtained by total synthesis. Many substances containing this cyclic moiety were described in view of their wide range of biological activities. Ertan et al. (2004) have synthesized a set of very active benzoxazoles againstBacillus subtilis, which also showed relevant antimicrobial activities againstEscherichia coli andCandida albicans [5]. Potent benzoxazoles showing HIV reverse transcriptase inhibition were reported by Medebielle and colleagues and these products presented antifungal activities againstAspergillus niger andA. flavus[6]. In 2008, Jauhari and colleagues described the cytotoxic activity of synthetic benzoxazoles against cervical, colon, and breast adenocarcinoma cell lines [7], while Arisoy and contributors described the relevant activities of 2,5-disubstituted benzoxazoles againstCandida krusei and gentamicin-resistant isolates ofPseudomonas aeruginosa [8]. Kim et al. (2010) have prepared benzoxazole amides, which presented good activity againstMalassezia furfur, a pathogenic fungus, involved in fatal sepsis cases [9]. Recently, Kuroyanagi and colleagues showed the outstanding potential of some 1,3-benzoxazol-4-carbonitriles againstCandida strains [10].At the same time, eugenol (1), a natural allylphenol found mainly in cloves [Eugenia caryophyllataL. Merr. & Perry (Myrtaceae)], shows relevant pharmacological activities, especially the antibacterial and antifungal actions [11]. Some authors have described the synthesis of eugenol derivatives in order to optimize its activities. The antimicrobial actions of eugenol seem to be related to cellular membranes interferences [12–14].Following our interest in obtaining bioactive eugenol derivatives and in face of the potential of benzoxazole derivatives as antimicrobial agents, we present herein the synthesis and anti-Candida activity of eugenol-benzoxazole based hybrids. Besides eugenol, we also employed dihydroeugenol as a starting material, in order to evaluate the influence of the allylic chain on antifungal activity.


## 2. Materials and Methods

### 2.1. Chemistry

Thin-layer chromatography (TLC) on silica gel-G plates (Macherey-Nagel, DC-Fertigfolien ALUGRAM® Xtra Sil G/UV254) was used to monitor reactions courses. Determination of the Rf values for the final products was done with the same TLC plates employing hexane/ethyl acetate (80 : 20, v/v) for all compounds. For column chromatography, column grade silica gel (Sorbiline; 0.040–0.063 mm mesh size) was employed. Melting points of the compounds were obtained on Microquímica MOAs 301 melting-point apparatus and are uncorrected. IR spectra were recorded on a Shimadzu FTIR-Affinity-1 spectrometer. NMR spectra were recorded on a Bruker AC-300 spectrometer (Rheinstetten, Germany) (300 MHz for 1H-NMR and 75 MHz for 13C-NMR spectra) in deuterated chloroform. Chemical shifts (δ) were reported in parts per million (ppm) with reference to tetramethylsilane (TMS) as internal standard and coupling constants (J) were reported in Hertz (Hz). High-resolution mass (HRMS) spectra were obtained for the final products on a quadrupole time-of-flight instrument (micrOTOF-QII, Bruker Daltonics, Billerica, MA, USA), equipped with an ESI positive and negative ion source.Synthesis of Nitroderivatives2and2′. To a solution of eugenol (1, 32.53 mmol) or dihydroeugenol (1′, 31.26 mmol) in dichloromethane (50 mL) were added equimolar amounts of sodium bisulfate and potassium nitrate, followed by addition of wet silica gel (1 : 1 w/w; 6.5 g for eugenol reaction and 6.3 g for dihydroeugenol reaction). The reaction was kept under vigorous magnetic stirring at room temperature for 48 hours and monitored by TLC (chloroform/methanol, 9.5 : 0.5, v/v). After completion of the reaction, the mixture was filtered off; the filtrate was dried over anhydrous sodium sulfate and concentrated under reduced pressure. The nitrocompounds2 and2′ were purified by column chromatography (hexane/ethyl acetate, 9 : 1, v/v) or by multiple extractions with hexane.4-Allyl-2-methoxy-6-nitrophenol (
2
). Yellow oil; 37% yield; IR (υ-máx, in cm−1): 3520 (O-H), 1600, 1550 (C=C), 1515, 1440 (NO2), 1270 (C-O-C); 1H NMR (δ; CDCl3; 300 MHz): 10.6 (s, 1H, O-H), 7.4 (d, 1H, H5, J = 1.8 Hz), 6.9 (d, 1H, H3, J = 1.8 Hz), 5.9–5.8 (m, 1H, CH2=CH-CH2), 5.1–5.0 (m, 2H, CH2=CH-CH2), 3.9 (s, 3H, OCH3), 3.3 (d, 2H, CH2=CH-CH2, J = 6.6 Hz); 13C NMR (δ; CDCl3; 75 MHz): 149.7 (C6), 144.7 (C1), 135.8 (CH2=CH-CH2), 133.5 (C2), 131.1 (C4), 118.5 (C5), 117.0 (CH2=CH-CH2), 114.9 (C3), 56.6 (OCH3), 39.3 (CH2=CH-CH2).2-Methoxy-6-nitro-4-propylphenol (
2′). Yellow oil; 62% yield; IR (υ-máx, in cm−1): 3250 (O-H), 1591 (C=C), 1541, 1448 (NO2), 1260 (C-O-C); 1H NMR (δ; CDCl3; 300 MHz): 10.6 (s, 1H, O-H), 7.4 (d, 1H, H5, J = 1.8 Hz), 6.9 (d, 1H, H3, J = 1.8 Hz), 3.9 (s, 3H, OCH3), 2.5 (t, 2H, CH3-CH2-CH2, J = 7.2 Hz), 1.7–1.5 (m, 2H, CH3-CH2-CH2), 0.9 (t, 3H, CH3-CH2-CH2, J = 7.2 Hz); 13C NMR (δ; CDCl3; 75 MHz): 149.6 (C6), 144.5 (C1), 133.7 (C4), 133.5 (C2), 118.6 (C5), 114.7 (C3), 56.6 (OCH3), 37.3 (CH3-CH2-CH2), 24.1 (CH3-CH2-CH2), 13.5 (CH3-CH2-CH2).Synthesis of Amino Derivatives3and3′. The nitrocompounds2 (11.87 mmol) or2′ (19.43 mmol) were solubilized in ethanol (100 mL) and to these solutions was added dihydrate stannous chloride (5 eq.). The mixture was kept under magnetic stirring at 70°C for 3 hours and the consumption of starting material was followed by TLC (chloroform/methanol, 9.5 : 0.5, v/v). The mixture was poured into ice water and aqueous NaHCO3 adjusted the pH to 7. The mixture was then extracted with ethyl acetate (10 × 20 mL); the organic layer was dried with anhydrous sodium sulfate and filtered, and the filtrate was concentrated under reduced pressure, which provided the pure products.4-Allyl-2-amino-6-methoxyphenol (
3
). Yellow crystals; 61% yield; m.p.: 95–98°C; IR (υ-máx, in cm−1): 3371, 3311 (NH2), 3074 (O-H), 1606, 1456 (C=C), 1512 (N-H), 1230 (C-O-C); 1H NMR (δ; CDCl3; 300 MHz): 6.2 (d, 1H, H5, J = 1.8 Hz), 6.1 (d, 1H, H3, J = 1.8 Hz), 6.0–5.8 (m, 1H, CH2=CH-CH2), 5.1–5.0 (m, 2H, CH2=CH-CH2), 3.8 (s, 3H, OCH3), 3,2 (d, 2H, CH2=CH-CH2, J = 6.9 Hz); 13C NMR (δ; CDCl3; 75 MHz): 146.6 (C6), 137.8 (CH2=CH-CH2), 133.8 (C2), 131.6 (C4), 131.2 (C1), 115.3 (CH2=CH-CH2), 109.4 (C5), 101.9 (C3), 55.9 (OCH3), 40.0 (CH2=CH-CH2).2-Amino-6-methoxy-4-propylphenol (
3′). Yellow crystals; 46% yield; m.p.: 63–66°C; IR (υ-máx, in cm−1): 3370, 3307 (NH2), 3012 (O-H), 1513 (N-H), 1212 (C-O-C); 1H NMR (δ; CDCl3; 300 MHz): 6.2 (d, 1H, H5, J = 1.8 Hz); 6.1 (d, 1H, H3, J = 1.8 Hz), 3.8 (s, 3H, OCH3), 2.4 (t, 2H, CH3-CH2-CH2, J = 7.2 Hz), 1.6–1.5 (m, 2H, CH3-CH2-CH2), 0.9 (t, 3H, CH3-CH2-CH2, J = 7.5 Hz); 13C NMR (δ; CDCl3; 75 MHz): 146.4 (C6), 134.3 (C2), 133.7 (C4), 130.8 (C1), 109.3 (C5), 101.7 (C3), 55,92 (OCH3), 37,99 (CH3-CH2-CH2), 24,74 (CH3-CH2-CH2), 13,82 (CH3-CH2-CH2).Synthesis of Amides
4a–4dand4a′–4d′. To a solution of3 or3′ (1 eq.) in diethyl ether (5 mL) was added 5 mL of aqueous NaHCO3 (1 eq.) and this mixture was stirred at 0°C for 5 min. Then, a solution of the corresponding acyl chloride (1 eq.) in 5 mL of ethyl ether was added dropwise over 30 minutes to the reaction mixture. Then the mixture was stirred at room temperature for 2 hours and the progress of the reaction was monitored by TLC (hexane/ethyl acetate, 7 : 3, v/v). The compound 4a was isolated by filtration and the others were isolated by extraction with dichloromethane, after evaporation of the diethyl ether. WhenO-acylation byproducts were detected, the crude product was treated with NaHCO3 (1 eq.) in aqueous methanol for 24 hours at room temperature. After deacylation (checked by TLC, hexane/ethyl acetate, 7 : 3, v/v), water was removed with anhydrous sodium sulfate, the mixture was filtered, and the solvent was removed under reduced pressure.N-(5-Allyl-2-hydroxy-3-methoxyphenyl)benzamide (
4a
). Light brown semi-solid; 40% yield; 1H NMR (δ; CDCl3; 300 MHz): 8.3 (s; 1H; N-H), 7.9–7.8 (m; 2H; H2′), 7.7 (d; 1H; H6; J = 1.8 Hz); 7.5–7.4 (m; 3H, H3′ e H4′), 6.5 (d; 1H; H4; J = 1.8 Hz), 6.0–5.8 (m; 1H; CH2=CH-CH2-), 5.1–5.0 (m; 2H; CH2=CH-CH2), 3.8 (s; 3H; OCH3), 3.3 (d; 2H; CH2=CH-CH2; J = 6.6 Hz). 13C NMR (δ; CDCl3; 75 MHz): 165.6 (C=O), 146.8 (C3), 137.5 (CH2=CH-CH2), 134.6 (C1′), 133.6 (C2), 131.9 (C5), 131.8 (C4′), 128.7 (C3′), 127.1 (C2′), 125.8 (C1), 115.7 (CH2=CH-CH2), 113.3 (C6), 107.2 (C4), 56.1 (OCH3), 40.2 (CH2=CH-CH2).N-(5-Allyl-2-hydroxy-3-methoxyphenyl)-4-chlorobenzamide (
4b
). Pink crystals; 64% yield; 1H NMR (δ; CDCl3; 300 MHz): 8.3 (s; 1H; N-H), 7.8 (d; 2H; H2′; J = 8.1 Hz); 7.7 (s; 1H; H6); 7.4 (d; 2H; H3′; J = 8.1 Hz), 6.5 (s; 1H; H4), 6.0–5.8 (m; 1H; CH2=CH-CH2-), 5.1–5.0 (m; 2H; CH2=CH-CH2), 3.8 (s; 3H; OCH3), 3.3 (d; 2H; CH2=CH-CH2; J = 6.6 Hz). 13C NMR (δ; CDCl3; 75 MHz): 164.4 (C=O), 146.6 (C3), 138.1 (C4′), 137.4 (CH2=CH-CH2), 133.5 (C2), 133.1 (C1′), 132.0 (C5), 128.9 (C2′), 128.5 (C3′), 125.5 (C1), 115.8 (CH2=CH-CH2), 113.2 (C6), 107.3 (C4), 56.1 (OCH3), 40.2 (CH2=CH-CH2).N-(5-Allyl-2-hydroxy-3-methoxyphenyl)-4-methoxybenzamide (
4c
). Orange crystals; 67% yield; 1H NMR (δ; CDCl3; 300 MHz): 7.9–7.8 (m; 2H; H2′), 7.6 (d; 1H; H6; J = 1.8 Hz), 7.0–6.9 (m; 2H; H3′), 6.5 (d; 1H; H4; J = 1.8 Hz), 6.0–5.8 (m; 1H; CH2=CH-CH2-), 5.1–5.0 (m; 2H; CH2=CH-CH2), 3.8 (s; 3H; C3-OCH3), 3.8 (s; 3H; C4′-OCH3), 3.3 (d; 2H; CH2=CH-CH2; J = 6.6 Hz). 13C NMR (δ; CDCl3; 75 MHz): 165.1 (C=O), 162.5 (C4′), 146.9 (C3), 137.5 (CH2=CH-CH2), 133.7 (C2), 131.9 (C5), 129.0 (C2′), 126.8 (C1), 125.9 (C1′), 115.7 (CH2=CH-CH2), 113.9 (C3′), 113.3 (C6), 107.1 (C4), 56.1 (C3-OCH3), 55.4 (C4′-OCH3), 40.2 (CH2=CH-CH2).N-(5-Allyl-2-hydroxy-3-methoxyphenyl)-4-nitrobenzamide (
4d
). Yellow crystals; 86% yield; 1H NMR (δ; CDCl3; 300 MHz): 8.3 (s; 1H; N-H), 8.3–8.2 (m; 2H; H3′), 8.1–8.0 (m; 2H; H2′), 7.7 (s; 1H, O-H), 6.5 (d; 1H; H6; J = 1.8 Hz), 6.0 (d; 1H; H4; J = 1.8 Hz), 6.0–5.8 (m; 1H; CH2=CH-CH2-), 5.1–5.0 (m; 2H; CH2=CH-CH2), 3.8 (s; 3H; OCH3), 3.3 (d; 2H; CH2=CH-CH2; J = 6.9 Hz). 13C NMR (δ; CDCl3; 75 MHz): 163.2 (C=O), 149.6 (C4′), 146.4 (C3), 140.3 (C1′), 137.3 (CH2=CH-CH2), 133.4 (C2), 132.2 (C5), 128.2 (C2′), 125.1 (C1), 123.9 (C3′), 115.9 (CH2=CH-CH2), 113.2 (C6), 107.6 (C4), 56.1 (OCH3), 40.1 (CH2=CH-CH2).N-(2-Hydroxy-3-methoxy-5-propylphenyl)benzamide (
4a′). Orange oil; 39% yield; 1H NMR (δ; CDCl3; 300 MHz): 8.3 (s; 1H; N-H), 7.9–7,8 (m; 2H; H2′), 7.7 (d; 1H; H6; J = 1.5 Hz), 7.5–7.4 (m; 2H; H3′ e H4′), 6.5 (d; 1H; H4; J = 1.8 Hz), 6.1 (s; 1H; O-H), 3.8 (s; 3H; OCH3), 2.5 (t; 2H; CH3-CH2-CH2-; J = 7.5 Hz), 1.7–1.5 (m; 2H; CH3-CH2-CH2-), 0.9 (t; 3H; CH3-CH2-CH2-; J = 7.2 Hz). 13C NMR (δ; CDCl3; 75 MHz): 165.5 (C=O), 146.5 (C3), 134.7 (C2), 133.6 (C1′), 133.2 (C5), 131.8 (C4′), 128.7 (C3′), 127.1 (C2′), 125.6 (C1), 113.0 (C6), 107.2 (C4), 56.1 (OCH3), 38.1 (CH3-CH2-CH2-), 24.8 (CH3-CH2-CH2-), 13.8 (CH3-CH2-CH2-).N-(2-Hydroxy-3-methoxy-5-propylphenyl)-4-chlorobenzamide (
4b′). White crystals; 37% yield; 1H NMR (δ; CDCl3; 300 MHz): 8.2 (s; 1H; N-H), 7.9–7.8 (m; 2H; H2′), 7.7 (d; 1H; H6; J = 1.8 Hz), 7.5–7.4 (m; 2H; H3′), 6.5 (d; 1H; H4; J = 1.8 Hz), 5.9 (s; 1H; O-H), 3.8 (s; 3H; OCH3), 2.5 (t; 2H; CH3-CH2-CH2-; J = 7.5 Hz), 1.7–1.5 (m; 2H; CH3-CH2-CH2-), 0.9 (t; 3H; CH3-CH2-CH2-; J = 7.2 Hz). 13C NMR (δ; CDCl3; 75 MHz): 164.3 (C=O), 146.3 (C3), 138.1 (C4′), 134.8 (C2), 133.2 (C1), 133.0 (C5), 129.0 (C2′), 128.5 (C3′), 125.4 (C1), 113.0 (C6), 107.2 (C4), 56.1 (OCH3), 38.1 (CH3-CH2-CH2-), 24.8 (CH3-CH2-CH2-), 13.7 (CH3-CH2-CH2-).N-(2-Hydroxy-3-methoxy-5-propylphenyl)-4-methoxybenzamide (
4c′). Brown crystals; 95% yield; 1H NMR (δ; CDCl3; 300 MHz): 8.2 (s; 1H; N-H), 7.9–7.8 (m; 2H; H2′), 7.6 (d; 1H; H6; J = 1.5 Hz), 7.0–6.9 (m; 2H; H3′), 6.5 (d; 1H; H4; J = 1.8 Hz), 3.9 (s; 3H; C3-OCH3), 3.8 (s; 3H; C4′-OCH3), 2.5 (t; 2H; CH3-CH2-CH2-; J = 7.5 Hz), 1.6–1.5 (m; 2H; CH3-CH2-CH2-), 0.9 (t; 3H; CH3-CH2-CH2-; J = 7.2 Hz). 13C NMR (δ; CDCl3; 75 MHz): 165.1 (C=O), 162.5 (C4′), 146.8 (C3), 134.6 (C2), 133.4 (C5), 129.0 (C2′), 126.9 (C1), 125.8 (C1′), 113.9 (C3′), 113.1 (C6), 107.1 (C4), 56.1 (C3-OCH3), 55.4 (C4′-OCH3), 38.1 (CH3-CH2-CH2-), 24.7 (CH3-CH2-CH2-), 13.7 (CH3-CH2-CH2-).N-(2-Hydroxy-3-methoxy-5-propylphenyl)-4-nitrobenzamide (
4d′). Orange crystals; 41% yield; 1H NMR (δ; CDCl3; 300 MHz): 8.4–8.3 (m; 2H; H3′), 8.1–8.0 (m; 2H; H2′), 7.8 (s; 1H; H6), 6.5 (d; 1H; H4; J = 1.8 Hz), 5.8 (s; 1H; O-H), 3.9 (s; 3H; OCH3), 2.5 (t; 2H; CH3-CH2-CH2-; J = 7.2 Hz), 1.7–1.5 (m; 2H; CH3-CH2-CH2-), 0.9 (t; 3H; CH3-CH2-CH2-; J = 7.2 Hz). 13C NMR (δ; CDCl3; 75 MHz): 163.1 (C=O), 149.7 (C4′), 146.1 (C3), 140.5 (C1′), 134.9 (C2), 132.9 (C5), 128.2 (C2′), 124.9 (C1), 124.0 (C3′), 112.9 (C6), 107.4 (C4), 56.1 (OCH3), 38.1 (CH3-CH2-CH2-), 24.8 (CH3-CH2-CH2-), 13.7 (CH3-CH2-CH2-).Synthesis of Benzoxazoles
5a–5dand5a′–5d′. To a solution of the corresponding amides (4a–4d,4a′–4d′, 1 eq.) in toluene (25 mL) was addedp-toluenesulfonic acid (3 eq.) and the mixture was stirred under reflux. The evolution of the reaction was monitored by TLC (hexane/ethyl acetate, 7 : 3, v/v). After the end of reaction, toluene was removed under reduced pressure and the crude product obtained was purified by filtration over silica gel with dichloromethane (compounds 4a, 4d, 4a′, 4b′, and 4d′) or by column chromatography (compounds 4b, 4c, 4c′, hexane/ethyl acetate, 9 : 1, v/v).5-Allyl-7-methoxy-2-phenylbenzo[d]oxazol (
5a
). Yellow semi-solid; 36% yield; Rf 0.8; IR (υ-máx, in cm−1): 1626 (C=N), 1603, 1585, 1449 (C=C), 1321, 1202 (C-N), 1132 (C-O-C); 1H NMR (δ; CDCl3; 300 MHz): 8.2-8.2 (m, 2H, H2′), 7.5-7.5 (m, 2H, H3′), 7.5 (m, 1H, H4′), 7.3–7.15 (m, 1H, H4), 6.7 (d, 1H, H6; J = 1.5 Hz), 6.0–5.9 (m, 1H, CH2=CH-CH2), 5.1–5.0 (m, 2H, CH2=CH-CH2), 4.0 (s, 3H, OCH3), 3.2 (d, 2H, CH2=CH-CH2; J = 6.6 Hz); 13C NMR (δ; CDCl3; 75 MHz): 144.3 (C2), 143.9 (C7), 137.5 (C3a), 137.3 (CH2=CH-CH2), 131.4 (C4′), 128.8 (C3′), 127.6 (C2′), 127.1 (C5), 125.7 (C7a), 125.6 (C1′), 116.0 (CH2=CH-CH2), 112.0 (C6), 108.8 (C4), 56.3 (OCH3), 40.5 (CH2=CH-CH2). HRMS-ESI:m/z calcd for C17H15O2N (M+H)+: 266.1176; found: 266.1184.5-Allyl-2-(4-chlorophenyl)-7-methoxy-benzo[d]oxazol (
5b
). Beige crystals; 23% yield; m.p.: 103.8–105.5°C; Rf 0.83; IR (υ-máx, in cm−1): 1624 (C=N), 1596, 1578, 1495 (C=C), 1317, 1199 (C-N), 1131 (C-O-C); 1H NMR (δ; CDCl3; 300 MHz): 8.2–8.1 (m, 2H, H2′), 7.4-7.4 (m, 2H, H3′), 7.2–6.09 (m, 1H, H4), 6.7 (d, 1H, H6; J = 1.2 Hz), 6.0–5.9 (m, 1H, CH2=CH-CH2), 5.1–5.0 (m, 2H, CH2=CH-CH2), 4.0 (s, 3H, OCH3), 3.4 (d, 2H, CH2=CH-CH2; J = 6.6 Hz); 13C NMR (δ; CDCl3; 75 MHz): 162.0 (C2), 144.3 (C7), 143.8 (C3a), 138.5 (C5), 137.7 (C4′), 137.6 (C7a), 137.2 (CH2=CH-CH2), 129.1 (C3′), 128.8 (C2′), 125.6 (C1′), 116.1 (CH2=CH-CH2), 112.0 (C6), 108.9 (C4), 56.3 (OCH3), 40.5 (CH2=CH-CH2). HRMS-ESI:m/z calcd for C17H14O2NCl (M+H)+: 300.0785; found: 300.0794.5-Allyl-7-methoxy-2-(4-methoxyphenyl)benzo[d]oxazol (
5c
). Yellow crystals; 20% yield; m.p.: 81.3–84.1°C; Rf 0.66; IR (υ-máx, in cm−1): 1622 (C=N), 1603, 1582, 1492 (C=C), 1322, 1248 (C-N), 1131 (C-O-C); 1H NMR (δ; CDCl3; 300 MHz): 8.2–8.1 (m, 2H, H2′), 7.2–7.0 (m, 1H, H4), 7.0–6.9 (m, 2H, H3′), 6.6 (d, 1H, H6; J = 1.2 Hz), 6.0–5.9 (m, 1H, CH2=CH-CH2), 5.1–5.0 (m, 2H, CH2=CH-CH2), 4.0 (s, 3H, C7-OCH3), 3.8 (s, 3H, C4-OCH3), 3.4 (d, 2H, CH2=CH-CH2; J = 6.6 Hz); 13C NMR (δ; CDCl3; 75 MHz): 163.2 (C2), 162.2 (C4′), 144.2 (C7), 144.0 (C3a), 137.4 (CH2=CH-CH2), 137.3 (C5), 129.3 (C2′), 119.6 (C1′), 115.9 (CH2=CH-CH2), 114.2 (C3′), 111.7 (C6), 108.4 (C4), 56.3 (C7-OCH3), 55.4 (C4′-OCH3), 40.5 (CH2=CH-CH2). HRMS-ESI:m/z calcd for C18H17O3N (M+H)+: 296.1281; found: 296.1291.5-Allyl-7-methoxy-2-(4-nitrophenyl)benzo[d]oxazol (
5d
). Yellow crystals; 81% yield; m.p.: 161.2–164°C; Rf 0.76; IR (υ-máx, in cm−1): 1618 (C=N), 1604, 1459 (C=C), 1518, 1342 (NO2), 1319, 1202 (C-N), 1126 (C-O-C); 1H NMR (δ; CDCl3; 300 MHz): 8.4–8.3 (m, 4H, H2′ e H3′), 7.2 (s, 1H, H4), 6.7 (s, 1H, H6), 6,0–5.9 (m, 1H, CH2=CH-CH2), 5.1-5.1 (m, 2H, CH2=CH-CH2), 4.0 (s, 3H, OCH3), 3.5 (d, 2H, CH2=CH-CH2; J = 6.6 Hz); 13C NMR (δ; CDCl3; 75 MHz): 160.6 (C2), 149.2 (C4′), 144.5 (C7), 143.6 (C3a), 138.9 (C5), 138.3 (C1′), 137.0 (CH2=CH-CH2), 132.6 (C7a), 128.3 (C3′), 124.0 (C2′), 116.3 (CH2=CH-CH2), 112.3 (C4), 109.5 (C6), 56.3 (OCH3), 40.4 (CH2=CH-CH2). HRMS-ESI:m/z calcd for C17H14O4N2 (M+H)+: 311.1026; found: 311.1024.7-Methoxy-2-phenyl-5-propylbenzo[d]oxazol (
5a′). Yellow semi-solid; 27% yield; Rf 0.79; IR (υ-máx, in cm−1): 1623 (C=N), 1601, 1496, 1465 (C=C), 1309, 1199 (C-N), 1129 (C-O-C); 1H NMR (δ; CDCl3; 300 MHz): 8.2-8.2 (m, 2H, H2′), 7.5–7.4 (m, 3H, H3′ e H4′), 7.21–7.02 (m, 1H, H4), 6.7 (d, 1H, H6; J = 1.2 Hz), 4.0 (s, 3H, OCH3), 2.6 (t, 2H, CH3-CH2-CH2; J = 7.2 Hz), 1.7–1.6 (m, 2H, CH3-CH2-CH2), 0.9 (t, 3H, CH3-CH2-CH2; J = 7.2 Hz); 13C NMR (δ; CDCl3; 75 MHz): 162.9 (C2), 144.1 (C7), 143.8 (C3a), 140.2 (C5), 131.3 (C4′), 128.7 (C3′), 128.4 (C7a), 127.5 (C2′), 127.1 (C1′), 111.6 (C6), 108.8 (C4), 56.3 (OCH3), 38.4 (CH3-CH2-CH2), 24.9 (CH3-CH2-CH2), 13.7 (CH3-CH2-CH2). HRMS-ESI:m/z calcd for C17H17O2N (M+H)+: 268.1332; found: 268.1338.2-(4-Chlorophenyl)-7-methoxy-5-propylbenzo[d]oxazol (
5b′). Light brown crystals; 26% yield; m.p.: 81.5–84.5°C; Rf 0.81; IR (υ-máx, in cm−1): 1626 (C=N), 1596, 1577, 1493, 1456 (C=C), 1319, 1201 (C-N), 1132 (C-O-C); 1H NMR (δ; CDCl3; 300 MHz): 8.2–8.1 (m, 2H, H2′), 7.5–7.4 (m, 2H, H3′), 7.2–7.0 (m, 1H, H4), 6.7 (d, 1H, H6; J = 1.2 Hz), 4.0 (s, 3H, OCH3), 2.6 (t, 2H, CH3-CH2-CH2; J = 7.2 Hz), 1.7–1.6 (m, 2H, CH3-CH2-CH2), 0.9 (t, 3H, CH3-CH2-CH2; J = 7.2 Hz); 13C NMR (δ; CDCl3; 75 MHz): 161,9 (C2), 144.2 (C7), 143.6 (C3a), 140.4 (C5), 137.5 (C7a), 129.1 (C3′), 128.8 (C2′), 125.6 (C4′), 121.7 (C1′), 111.7 (C6), 108.9 (C4), 56.3 (OCH3), 38.4 (CH3-CH2-CH2), 24.9 (CH3-CH2-CH2), 13.7 (CH3-CH2-CH2). HRMS-ESI:m/z calcd for C17H16O2NCl (M+H)+: 302.0942; found: 302.0944.7-Methoxy-2-(4-methoxyphenyl)-5-propylbenzo[d]oxazol (
5c′). Brown crystals; 30% yield; m.p.: 89.4–92.5°C; Rf 0.59; IR (υ-máx, in cm−1): 1622 (C=N), 1605, 1583, 1496 (C=C), 1324, 1255 (C-N), 1132 (C-O-C); 1H NMR (δ; CDCl3; 300 MHz): 8.2–8.1 (m, 2H, H2′), 7.22–7.1 (m, 1H, H4), 7.0–6.9 (m, 2H, H3′), 6.6 (d, 1H, H6; J = 1.2 Hz), 4.0 (s, 3H, C7-OCH3), 3.8 (s, 3H, C4′-OCH3), 2.6 (t, 2H, CH3-CH2-CH2; J = 7.5 Hz), 1.7–1.6 (m, 2H, CH3-CH2-CH2), 0.9 (t, 3H, CH3-CH2-CH2; J = 7.5 Hz); 13C NMR (δ; CDCl3; 75 MHz): 163.0 (C2), 162.1 (C4′), 144.0 (C7), 143.9 (C3a), 140.0 (C5), 138.0 (C7a), 129.3 (C2′), 119.7 (C1′), 114.2 (C3′), 111.4 (C6), 108.4 (C4), 56.3 (C7-OCH3), 55.4 (C4′-OCH3), 38.4 (CH3-CH2-CH2), 24.9 (CH3-CH2-CH2), 13.7 (CH3-CH2-CH2). HRMS-ESI:m/z calcd for C18H19O3N (M+H)+: 298.1438; found: 298.1450.7-Methoxy-2-(4-nitrophenyl)-5-propylbenzo[d]oxazol (
5d′). Yellow crystals; 33% yield; m.p.: 136.5–140°C; Rf 0.72; IR (υ-máx, in cm−1): 1625 (C=N), 1602 (C=C), 1518, 1339 (NO2), 1309, 1200 (C-N), 1132 (C-O-C); 1H NMR (δ; CDCl3; 300 MHz): 8.4–8.3 (m, 4H, H2′ e H3′), 7.2 (s, 1H, H4), 6.7 (s, 1H, H6), 4.0 (s, 3H, OCH3), 2.7 (t, 2H, CH3-CH2-CH2; J = 7.5 Hz), 1.7–1.6 (m, 2H, CH3-CH2-CH2), 0.9 (t, 3H, CH3-CH2-CH2; J = 7.5 Hz); 13C NMR (δ; CDCl3; 75 MHz): 160.5 (C2), 149.2 (C4′), 144.3 (C7), 143.5 (C3a), 141.0 (C1′), 138.7 (C5), 132.7 (C7a), 128.3 (C2′), 124.1 (C3′), 112.3 (C6), 109.6 (C4), 56.3 (OCH3), 38.4 (CH3-CH2-CH2), 24.8 (CH3-CH2-CH2), 13.7 (CH3-CH2-CH2). HRMS-ESI:m/z calcd for C17H16O4N2 (M+H)+: 313.1183; found: 313.1182.


### 2.2. Antifungal Activity Evaluation

The antifungal activity evaluation was performed through a Mueller-Hinton broth microdilution method and with the methodology and interpretative criteria proposed by document M27A3 [15]. The stock solutions of all the compounds were prepared in DMSO 1% at final concentration and tested at concentrations (µg/mL) 100; 60; 30; 15; 7.5; 3.75; 1.875; 0.468; 0.23; 0.06. The standard drug fluconazole was applied as control of fungistatic action. Results were visualized and analyzed at 530 nm in an Anthos Zenyth 200rt Microplate Reader. The inhibitory concentrations of microbial growth were determined at 50% (IC50) and 90% (IC90) in µmol/mL and compared among the microorganisms. The tests were all done in duplicate.


### 2.3. Cytotoxicity Assay

The cytotoxicity of the compounds (200–1.5μg/mL) to peripheral blood mononuclear cells (PBMCs) was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) method. The PBMCs were obtained from healthy volunteers by Ficoll-Hypaque density gradient centrifugation. These cells were grown in RMPI medium (Cultilab, Brazil) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS) (Cultilab, Brazil), 100 mg/mL penicillin, and 100 mg/mL streptomycin in 5% CO2 atmosphere at 37°C. The cell suspension of PBMCs at a concentration of 2.4 × 106 cells/mL was distributed in a 96-well plate, 90 μL in each well with 10 μL of test compounds at different concentrations, and incubated at 37°C in an incubator at 5% CO2 for 48 h. After that, 10 μL of MTT dye was added (5 mg/mL) and the cells were incubated again for an additional 4 h period. Then, the medium was carefully removed and 100 μL of DMSO was added for solubilization of formazan crystals. The plates were shaken for 5 min, and absorbance for each sample was measured in a spectrophotometric microplate reader at 560 nm. The percentage of cytotoxicity was calculated as [(A-B)/A×100], whereA andB are the absorbance of control and treated cells, respectively. Data were analyzed using linear regression to obtain values for CC50 and CC90 (cytotoxic concentration for 50% and 90% of cells, resp.). Selectivity indexes were expressed as the ratio CC50/IC50. The tests were all done in duplicate.


## 2.1. Chemistry

Thin-layer chromatography (TLC) on silica gel-G plates (Macherey-Nagel, DC-Fertigfolien ALUGRAM® Xtra Sil G/UV254) was used to monitor reactions courses. Determination of the Rf values for the final products was done with the same TLC plates employing hexane/ethyl acetate (80 : 20, v/v) for all compounds. For column chromatography, column grade silica gel (Sorbiline; 0.040–0.063 mm mesh size) was employed. Melting points of the compounds were obtained on Microquímica MOAs 301 melting-point apparatus and are uncorrected. IR spectra were recorded on a Shimadzu FTIR-Affinity-1 spectrometer. NMR spectra were recorded on a Bruker AC-300 spectrometer (Rheinstetten, Germany) (300 MHz for 1H-NMR and 75 MHz for 13C-NMR spectra) in deuterated chloroform. Chemical shifts (δ) were reported in parts per million (ppm) with reference to tetramethylsilane (TMS) as internal standard and coupling constants (J) were reported in Hertz (Hz). High-resolution mass (HRMS) spectra were obtained for the final products on a quadrupole time-of-flight instrument (micrOTOF-QII, Bruker Daltonics, Billerica, MA, USA), equipped with an ESI positive and negative ion source.Synthesis of Nitroderivatives2and2′. To a solution of eugenol (1, 32.53 mmol) or dihydroeugenol (1′, 31.26 mmol) in dichloromethane (50 mL) were added equimolar amounts of sodium bisulfate and potassium nitrate, followed by addition of wet silica gel (1 : 1 w/w; 6.5 g for eugenol reaction and 6.3 g for dihydroeugenol reaction). The reaction was kept under vigorous magnetic stirring at room temperature for 48 hours and monitored by TLC (chloroform/methanol, 9.5 : 0.5, v/v). After completion of the reaction, the mixture was filtered off; the filtrate was dried over anhydrous sodium sulfate and concentrated under reduced pressure. The nitrocompounds2 and2′ were purified by column chromatography (hexane/ethyl acetate, 9 : 1, v/v) or by multiple extractions with hexane.4-Allyl-2-methoxy-6-nitrophenol (
2
). Yellow oil; 37% yield; IR (υ-máx, in cm−1): 3520 (O-H), 1600, 1550 (C=C), 1515, 1440 (NO2), 1270 (C-O-C); 1H NMR (δ; CDCl3; 300 MHz): 10.6 (s, 1H, O-H), 7.4 (d, 1H, H5, J = 1.8 Hz), 6.9 (d, 1H, H3, J = 1.8 Hz), 5.9–5.8 (m, 1H, CH2=CH-CH2), 5.1–5.0 (m, 2H, CH2=CH-CH2), 3.9 (s, 3H, OCH3), 3.3 (d, 2H, CH2=CH-CH2, J = 6.6 Hz); 13C NMR (δ; CDCl3; 75 MHz): 149.7 (C6), 144.7 (C1), 135.8 (CH2=CH-CH2), 133.5 (C2), 131.1 (C4), 118.5 (C5), 117.0 (CH2=CH-CH2), 114.9 (C3), 56.6 (OCH3), 39.3 (CH2=CH-CH2).2-Methoxy-6-nitro-4-propylphenol (
2′). Yellow oil; 62% yield; IR (υ-máx, in cm−1): 3250 (O-H), 1591 (C=C), 1541, 1448 (NO2), 1260 (C-O-C); 1H NMR (δ; CDCl3; 300 MHz): 10.6 (s, 1H, O-H), 7.4 (d, 1H, H5, J = 1.8 Hz), 6.9 (d, 1H, H3, J = 1.8 Hz), 3.9 (s, 3H, OCH3), 2.5 (t, 2H, CH3-CH2-CH2, J = 7.2 Hz), 1.7–1.5 (m, 2H, CH3-CH2-CH2), 0.9 (t, 3H, CH3-CH2-CH2, J = 7.2 Hz); 13C NMR (δ; CDCl3; 75 MHz): 149.6 (C6), 144.5 (C1), 133.7 (C4), 133.5 (C2), 118.6 (C5), 114.7 (C3), 56.6 (OCH3), 37.3 (CH3-CH2-CH2), 24.1 (CH3-CH2-CH2), 13.5 (CH3-CH2-CH2).Synthesis of Amino Derivatives3and3′. The nitrocompounds2 (11.87 mmol) or2′ (19.43 mmol) were solubilized in ethanol (100 mL) and to these solutions was added dihydrate stannous chloride (5 eq.). The mixture was kept under magnetic stirring at 70°C for 3 hours and the consumption of starting material was followed by TLC (chloroform/methanol, 9.5 : 0.5, v/v). The mixture was poured into ice water and aqueous NaHCO3 adjusted the pH to 7. The mixture was then extracted with ethyl acetate (10 × 20 mL); the organic layer was dried with anhydrous sodium sulfate and filtered, and the filtrate was concentrated under reduced pressure, which provided the pure products.4-Allyl-2-amino-6-methoxyphenol (
3
). Yellow crystals; 61% yield; m.p.: 95–98°C; IR (υ-máx, in cm−1): 3371, 3311 (NH2), 3074 (O-H), 1606, 1456 (C=C), 1512 (N-H), 1230 (C-O-C); 1H NMR (δ; CDCl3; 300 MHz): 6.2 (d, 1H, H5, J = 1.8 Hz), 6.1 (d, 1H, H3, J = 1.8 Hz), 6.0–5.8 (m, 1H, CH2=CH-CH2), 5.1–5.0 (m, 2H, CH2=CH-CH2), 3.8 (s, 3H, OCH3), 3,2 (d, 2H, CH2=CH-CH2, J = 6.9 Hz); 13C NMR (δ; CDCl3; 75 MHz): 146.6 (C6), 137.8 (CH2=CH-CH2), 133.8 (C2), 131.6 (C4), 131.2 (C1), 115.3 (CH2=CH-CH2), 109.4 (C5), 101.9 (C3), 55.9 (OCH3), 40.0 (CH2=CH-CH2).2-Amino-6-methoxy-4-propylphenol (
3′). Yellow crystals; 46% yield; m.p.: 63–66°C; IR (υ-máx, in cm−1): 3370, 3307 (NH2), 3012 (O-H), 1513 (N-H), 1212 (C-O-C); 1H NMR (δ; CDCl3; 300 MHz): 6.2 (d, 1H, H5, J = 1.8 Hz); 6.1 (d, 1H, H3, J = 1.8 Hz), 3.8 (s, 3H, OCH3), 2.4 (t, 2H, CH3-CH2-CH2, J = 7.2 Hz), 1.6–1.5 (m, 2H, CH3-CH2-CH2), 0.9 (t, 3H, CH3-CH2-CH2, J = 7.5 Hz); 13C NMR (δ; CDCl3; 75 MHz): 146.4 (C6), 134.3 (C2), 133.7 (C4), 130.8 (C1), 109.3 (C5), 101.7 (C3), 55,92 (OCH3), 37,99 (CH3-CH2-CH2), 24,74 (CH3-CH2-CH2), 13,82 (CH3-CH2-CH2).Synthesis of Amides
4a–4dand4a′–4d′. To a solution of3 or3′ (1 eq.) in diethyl ether (5 mL) was added 5 mL of aqueous NaHCO3 (1 eq.) and this mixture was stirred at 0°C for 5 min. Then, a solution of the corresponding acyl chloride (1 eq.) in 5 mL of ethyl ether was added dropwise over 30 minutes to the reaction mixture. Then the mixture was stirred at room temperature for 2 hours and the progress of the reaction was monitored by TLC (hexane/ethyl acetate, 7 : 3, v/v). The compound 4a was isolated by filtration and the others were isolated by extraction with dichloromethane, after evaporation of the diethyl ether. WhenO-acylation byproducts were detected, the crude product was treated with NaHCO3 (1 eq.) in aqueous methanol for 24 hours at room temperature. After deacylation (checked by TLC, hexane/ethyl acetate, 7 : 3, v/v), water was removed with anhydrous sodium sulfate, the mixture was filtered, and the solvent was removed under reduced pressure.N-(5-Allyl-2-hydroxy-3-methoxyphenyl)benzamide (
4a
). Light brown semi-solid; 40% yield; 1H NMR (δ; CDCl3; 300 MHz): 8.3 (s; 1H; N-H), 7.9–7.8 (m; 2H; H2′), 7.7 (d; 1H; H6; J = 1.8 Hz); 7.5–7.4 (m; 3H, H3′ e H4′), 6.5 (d; 1H; H4; J = 1.8 Hz), 6.0–5.8 (m; 1H; CH2=CH-CH2-), 5.1–5.0 (m; 2H; CH2=CH-CH2), 3.8 (s; 3H; OCH3), 3.3 (d; 2H; CH2=CH-CH2; J = 6.6 Hz). 13C NMR (δ; CDCl3; 75 MHz): 165.6 (C=O), 146.8 (C3), 137.5 (CH2=CH-CH2), 134.6 (C1′), 133.6 (C2), 131.9 (C5), 131.8 (C4′), 128.7 (C3′), 127.1 (C2′), 125.8 (C1), 115.7 (CH2=CH-CH2), 113.3 (C6), 107.2 (C4), 56.1 (OCH3), 40.2 (CH2=CH-CH2).N-(5-Allyl-2-hydroxy-3-methoxyphenyl)-4-chlorobenzamide (
4b
). Pink crystals; 64% yield; 1H NMR (δ; CDCl3; 300 MHz): 8.3 (s; 1H; N-H), 7.8 (d; 2H; H2′; J = 8.1 Hz); 7.7 (s; 1H; H6); 7.4 (d; 2H; H3′; J = 8.1 Hz), 6.5 (s; 1H; H4), 6.0–5.8 (m; 1H; CH2=CH-CH2-), 5.1–5.0 (m; 2H; CH2=CH-CH2), 3.8 (s; 3H; OCH3), 3.3 (d; 2H; CH2=CH-CH2; J = 6.6 Hz). 13C NMR (δ; CDCl3; 75 MHz): 164.4 (C=O), 146.6 (C3), 138.1 (C4′), 137.4 (CH2=CH-CH2), 133.5 (C2), 133.1 (C1′), 132.0 (C5), 128.9 (C2′), 128.5 (C3′), 125.5 (C1), 115.8 (CH2=CH-CH2), 113.2 (C6), 107.3 (C4), 56.1 (OCH3), 40.2 (CH2=CH-CH2).N-(5-Allyl-2-hydroxy-3-methoxyphenyl)-4-methoxybenzamide (
4c
). Orange crystals; 67% yield; 1H NMR (δ; CDCl3; 300 MHz): 7.9–7.8 (m; 2H; H2′), 7.6 (d; 1H; H6; J = 1.8 Hz), 7.0–6.9 (m; 2H; H3′), 6.5 (d; 1H; H4; J = 1.8 Hz), 6.0–5.8 (m; 1H; CH2=CH-CH2-), 5.1–5.0 (m; 2H; CH2=CH-CH2), 3.8 (s; 3H; C3-OCH3), 3.8 (s; 3H; C4′-OCH3), 3.3 (d; 2H; CH2=CH-CH2; J = 6.6 Hz). 13C NMR (δ; CDCl3; 75 MHz): 165.1 (C=O), 162.5 (C4′), 146.9 (C3), 137.5 (CH2=CH-CH2), 133.7 (C2), 131.9 (C5), 129.0 (C2′), 126.8 (C1), 125.9 (C1′), 115.7 (CH2=CH-CH2), 113.9 (C3′), 113.3 (C6), 107.1 (C4), 56.1 (C3-OCH3), 55.4 (C4′-OCH3), 40.2 (CH2=CH-CH2).N-(5-Allyl-2-hydroxy-3-methoxyphenyl)-4-nitrobenzamide (
4d
). Yellow crystals; 86% yield; 1H NMR (δ; CDCl3; 300 MHz): 8.3 (s; 1H; N-H), 8.3–8.2 (m; 2H; H3′), 8.1–8.0 (m; 2H; H2′), 7.7 (s; 1H, O-H), 6.5 (d; 1H; H6; J = 1.8 Hz), 6.0 (d; 1H; H4; J = 1.8 Hz), 6.0–5.8 (m; 1H; CH2=CH-CH2-), 5.1–5.0 (m; 2H; CH2=CH-CH2), 3.8 (s; 3H; OCH3), 3.3 (d; 2H; CH2=CH-CH2; J = 6.9 Hz). 13C NMR (δ; CDCl3; 75 MHz): 163.2 (C=O), 149.6 (C4′), 146.4 (C3), 140.3 (C1′), 137.3 (CH2=CH-CH2), 133.4 (C2), 132.2 (C5), 128.2 (C2′), 125.1 (C1), 123.9 (C3′), 115.9 (CH2=CH-CH2), 113.2 (C6), 107.6 (C4), 56.1 (OCH3), 40.1 (CH2=CH-CH2).N-(2-Hydroxy-3-methoxy-5-propylphenyl)benzamide (
4a′). Orange oil; 39% yield; 1H NMR (δ; CDCl3; 300 MHz): 8.3 (s; 1H; N-H), 7.9–7,8 (m; 2H; H2′), 7.7 (d; 1H; H6; J = 1.5 Hz), 7.5–7.4 (m; 2H; H3′ e H4′), 6.5 (d; 1H; H4; J = 1.8 Hz), 6.1 (s; 1H; O-H), 3.8 (s; 3H; OCH3), 2.5 (t; 2H; CH3-CH2-CH2-; J = 7.5 Hz), 1.7–1.5 (m; 2H; CH3-CH2-CH2-), 0.9 (t; 3H; CH3-CH2-CH2-; J = 7.2 Hz). 13C NMR (δ; CDCl3; 75 MHz): 165.5 (C=O), 146.5 (C3), 134.7 (C2), 133.6 (C1′), 133.2 (C5), 131.8 (C4′), 128.7 (C3′), 127.1 (C2′), 125.6 (C1), 113.0 (C6), 107.2 (C4), 56.1 (OCH3), 38.1 (CH3-CH2-CH2-), 24.8 (CH3-CH2-CH2-), 13.8 (CH3-CH2-CH2-).N-(2-Hydroxy-3-methoxy-5-propylphenyl)-4-chlorobenzamide (
4b′). White crystals; 37% yield; 1H NMR (δ; CDCl3; 300 MHz): 8.2 (s; 1H; N-H), 7.9–7.8 (m; 2H; H2′), 7.7 (d; 1H; H6; J = 1.8 Hz), 7.5–7.4 (m; 2H; H3′), 6.5 (d; 1H; H4; J = 1.8 Hz), 5.9 (s; 1H; O-H), 3.8 (s; 3H; OCH3), 2.5 (t; 2H; CH3-CH2-CH2-; J = 7.5 Hz), 1.7–1.5 (m; 2H; CH3-CH2-CH2-), 0.9 (t; 3H; CH3-CH2-CH2-; J = 7.2 Hz). 13C NMR (δ; CDCl3; 75 MHz): 164.3 (C=O), 146.3 (C3), 138.1 (C4′), 134.8 (C2), 133.2 (C1), 133.0 (C5), 129.0 (C2′), 128.5 (C3′), 125.4 (C1), 113.0 (C6), 107.2 (C4), 56.1 (OCH3), 38.1 (CH3-CH2-CH2-), 24.8 (CH3-CH2-CH2-), 13.7 (CH3-CH2-CH2-).N-(2-Hydroxy-3-methoxy-5-propylphenyl)-4-methoxybenzamide (
4c′). Brown crystals; 95% yield; 1H NMR (δ; CDCl3; 300 MHz): 8.2 (s; 1H; N-H), 7.9–7.8 (m; 2H; H2′), 7.6 (d; 1H; H6; J = 1.5 Hz), 7.0–6.9 (m; 2H; H3′), 6.5 (d; 1H; H4; J = 1.8 Hz), 3.9 (s; 3H; C3-OCH3), 3.8 (s; 3H; C4′-OCH3), 2.5 (t; 2H; CH3-CH2-CH2-; J = 7.5 Hz), 1.6–1.5 (m; 2H; CH3-CH2-CH2-), 0.9 (t; 3H; CH3-CH2-CH2-; J = 7.2 Hz). 13C NMR (δ; CDCl3; 75 MHz): 165.1 (C=O), 162.5 (C4′), 146.8 (C3), 134.6 (C2), 133.4 (C5), 129.0 (C2′), 126.9 (C1), 125.8 (C1′), 113.9 (C3′), 113.1 (C6), 107.1 (C4), 56.1 (C3-OCH3), 55.4 (C4′-OCH3), 38.1 (CH3-CH2-CH2-), 24.7 (CH3-CH2-CH2-), 13.7 (CH3-CH2-CH2-).N-(2-Hydroxy-3-methoxy-5-propylphenyl)-4-nitrobenzamide (
4d′). Orange crystals; 41% yield; 1H NMR (δ; CDCl3; 300 MHz): 8.4–8.3 (m; 2H; H3′), 8.1–8.0 (m; 2H; H2′), 7.8 (s; 1H; H6), 6.5 (d; 1H; H4; J = 1.8 Hz), 5.8 (s; 1H; O-H), 3.9 (s; 3H; OCH3), 2.5 (t; 2H; CH3-CH2-CH2-; J = 7.2 Hz), 1.7–1.5 (m; 2H; CH3-CH2-CH2-), 0.9 (t; 3H; CH3-CH2-CH2-; J = 7.2 Hz). 13C NMR (δ; CDCl3; 75 MHz): 163.1 (C=O), 149.7 (C4′), 146.1 (C3), 140.5 (C1′), 134.9 (C2), 132.9 (C5), 128.2 (C2′), 124.9 (C1), 124.0 (C3′), 112.9 (C6), 107.4 (C4), 56.1 (OCH3), 38.1 (CH3-CH2-CH2-), 24.8 (CH3-CH2-CH2-), 13.7 (CH3-CH2-CH2-).Synthesis of Benzoxazoles
5a–5dand5a′–5d′. To a solution of the corresponding amides (4a–4d,4a′–4d′, 1 eq.) in toluene (25 mL) was addedp-toluenesulfonic acid (3 eq.) and the mixture was stirred under reflux. The evolution of the reaction was monitored by TLC (hexane/ethyl acetate, 7 : 3, v/v). After the end of reaction, toluene was removed under reduced pressure and the crude product obtained was purified by filtration over silica gel with dichloromethane (compounds 4a, 4d, 4a′, 4b′, and 4d′) or by column chromatography (compounds 4b, 4c, 4c′, hexane/ethyl acetate, 9 : 1, v/v).5-Allyl-7-methoxy-2-phenylbenzo[d]oxazol (
5a
). Yellow semi-solid; 36% yield; Rf 0.8; IR (υ-máx, in cm−1): 1626 (C=N), 1603, 1585, 1449 (C=C), 1321, 1202 (C-N), 1132 (C-O-C); 1H NMR (δ; CDCl3; 300 MHz): 8.2-8.2 (m, 2H, H2′), 7.5-7.5 (m, 2H, H3′), 7.5 (m, 1H, H4′), 7.3–7.15 (m, 1H, H4), 6.7 (d, 1H, H6; J = 1.5 Hz), 6.0–5.9 (m, 1H, CH2=CH-CH2), 5.1–5.0 (m, 2H, CH2=CH-CH2), 4.0 (s, 3H, OCH3), 3.2 (d, 2H, CH2=CH-CH2; J = 6.6 Hz); 13C NMR (δ; CDCl3; 75 MHz): 144.3 (C2), 143.9 (C7), 137.5 (C3a), 137.3 (CH2=CH-CH2), 131.4 (C4′), 128.8 (C3′), 127.6 (C2′), 127.1 (C5), 125.7 (C7a), 125.6 (C1′), 116.0 (CH2=CH-CH2), 112.0 (C6), 108.8 (C4), 56.3 (OCH3), 40.5 (CH2=CH-CH2). HRMS-ESI:m/z calcd for C17H15O2N (M+H)+: 266.1176; found: 266.1184.5-Allyl-2-(4-chlorophenyl)-7-methoxy-benzo[d]oxazol (
5b
). Beige crystals; 23% yield; m.p.: 103.8–105.5°C; Rf 0.83; IR (υ-máx, in cm−1): 1624 (C=N), 1596, 1578, 1495 (C=C), 1317, 1199 (C-N), 1131 (C-O-C); 1H NMR (δ; CDCl3; 300 MHz): 8.2–8.1 (m, 2H, H2′), 7.4-7.4 (m, 2H, H3′), 7.2–6.09 (m, 1H, H4), 6.7 (d, 1H, H6; J = 1.2 Hz), 6.0–5.9 (m, 1H, CH2=CH-CH2), 5.1–5.0 (m, 2H, CH2=CH-CH2), 4.0 (s, 3H, OCH3), 3.4 (d, 2H, CH2=CH-CH2; J = 6.6 Hz); 13C NMR (δ; CDCl3; 75 MHz): 162.0 (C2), 144.3 (C7), 143.8 (C3a), 138.5 (C5), 137.7 (C4′), 137.6 (C7a), 137.2 (CH2=CH-CH2), 129.1 (C3′), 128.8 (C2′), 125.6 (C1′), 116.1 (CH2=CH-CH2), 112.0 (C6), 108.9 (C4), 56.3 (OCH3), 40.5 (CH2=CH-CH2). HRMS-ESI:m/z calcd for C17H14O2NCl (M+H)+: 300.0785; found: 300.0794.5-Allyl-7-methoxy-2-(4-methoxyphenyl)benzo[d]oxazol (
5c
). Yellow crystals; 20% yield; m.p.: 81.3–84.1°C; Rf 0.66; IR (υ-máx, in cm−1): 1622 (C=N), 1603, 1582, 1492 (C=C), 1322, 1248 (C-N), 1131 (C-O-C); 1H NMR (δ; CDCl3; 300 MHz): 8.2–8.1 (m, 2H, H2′), 7.2–7.0 (m, 1H, H4), 7.0–6.9 (m, 2H, H3′), 6.6 (d, 1H, H6; J = 1.2 Hz), 6.0–5.9 (m, 1H, CH2=CH-CH2), 5.1–5.0 (m, 2H, CH2=CH-CH2), 4.0 (s, 3H, C7-OCH3), 3.8 (s, 3H, C4-OCH3), 3.4 (d, 2H, CH2=CH-CH2; J = 6.6 Hz); 13C NMR (δ; CDCl3; 75 MHz): 163.2 (C2), 162.2 (C4′), 144.2 (C7), 144.0 (C3a), 137.4 (CH2=CH-CH2), 137.3 (C5), 129.3 (C2′), 119.6 (C1′), 115.9 (CH2=CH-CH2), 114.2 (C3′), 111.7 (C6), 108.4 (C4), 56.3 (C7-OCH3), 55.4 (C4′-OCH3), 40.5 (CH2=CH-CH2). HRMS-ESI:m/z calcd for C18H17O3N (M+H)+: 296.1281; found: 296.1291.5-Allyl-7-methoxy-2-(4-nitrophenyl)benzo[d]oxazol (
5d
). Yellow crystals; 81% yield; m.p.: 161.2–164°C; Rf 0.76; IR (υ-máx, in cm−1): 1618 (C=N), 1604, 1459 (C=C), 1518, 1342 (NO2), 1319, 1202 (C-N), 1126 (C-O-C); 1H NMR (δ; CDCl3; 300 MHz): 8.4–8.3 (m, 4H, H2′ e H3′), 7.2 (s, 1H, H4), 6.7 (s, 1H, H6), 6,0–5.9 (m, 1H, CH2=CH-CH2), 5.1-5.1 (m, 2H, CH2=CH-CH2), 4.0 (s, 3H, OCH3), 3.5 (d, 2H, CH2=CH-CH2; J = 6.6 Hz); 13C NMR (δ; CDCl3; 75 MHz): 160.6 (C2), 149.2 (C4′), 144.5 (C7), 143.6 (C3a), 138.9 (C5), 138.3 (C1′), 137.0 (CH2=CH-CH2), 132.6 (C7a), 128.3 (C3′), 124.0 (C2′), 116.3 (CH2=CH-CH2), 112.3 (C4), 109.5 (C6), 56.3 (OCH3), 40.4 (CH2=CH-CH2). HRMS-ESI:m/z calcd for C17H14O4N2 (M+H)+: 311.1026; found: 311.1024.7-Methoxy-2-phenyl-5-propylbenzo[d]oxazol (
5a′). Yellow semi-solid; 27% yield; Rf 0.79; IR (υ-máx, in cm−1): 1623 (C=N), 1601, 1496, 1465 (C=C), 1309, 1199 (C-N), 1129 (C-O-C); 1H NMR (δ; CDCl3; 300 MHz): 8.2-8.2 (m, 2H, H2′), 7.5–7.4 (m, 3H, H3′ e H4′), 7.21–7.02 (m, 1H, H4), 6.7 (d, 1H, H6; J = 1.2 Hz), 4.0 (s, 3H, OCH3), 2.6 (t, 2H, CH3-CH2-CH2; J = 7.2 Hz), 1.7–1.6 (m, 2H, CH3-CH2-CH2), 0.9 (t, 3H, CH3-CH2-CH2; J = 7.2 Hz); 13C NMR (δ; CDCl3; 75 MHz): 162.9 (C2), 144.1 (C7), 143.8 (C3a), 140.2 (C5), 131.3 (C4′), 128.7 (C3′), 128.4 (C7a), 127.5 (C2′), 127.1 (C1′), 111.6 (C6), 108.8 (C4), 56.3 (OCH3), 38.4 (CH3-CH2-CH2), 24.9 (CH3-CH2-CH2), 13.7 (CH3-CH2-CH2). HRMS-ESI:m/z calcd for C17H17O2N (M+H)+: 268.1332; found: 268.1338.2-(4-Chlorophenyl)-7-methoxy-5-propylbenzo[d]oxazol (
5b′). Light brown crystals; 26% yield; m.p.: 81.5–84.5°C; Rf 0.81; IR (υ-máx, in cm−1): 1626 (C=N), 1596, 1577, 1493, 1456 (C=C), 1319, 1201 (C-N), 1132 (C-O-C); 1H NMR (δ; CDCl3; 300 MHz): 8.2–8.1 (m, 2H, H2′), 7.5–7.4 (m, 2H, H3′), 7.2–7.0 (m, 1H, H4), 6.7 (d, 1H, H6; J = 1.2 Hz), 4.0 (s, 3H, OCH3), 2.6 (t, 2H, CH3-CH2-CH2; J = 7.2 Hz), 1.7–1.6 (m, 2H, CH3-CH2-CH2), 0.9 (t, 3H, CH3-CH2-CH2; J = 7.2 Hz); 13C NMR (δ; CDCl3; 75 MHz): 161,9 (C2), 144.2 (C7), 143.6 (C3a), 140.4 (C5), 137.5 (C7a), 129.1 (C3′), 128.8 (C2′), 125.6 (C4′), 121.7 (C1′), 111.7 (C6), 108.9 (C4), 56.3 (OCH3), 38.4 (CH3-CH2-CH2), 24.9 (CH3-CH2-CH2), 13.7 (CH3-CH2-CH2). HRMS-ESI:m/z calcd for C17H16O2NCl (M+H)+: 302.0942; found: 302.0944.7-Methoxy-2-(4-methoxyphenyl)-5-propylbenzo[d]oxazol (
5c′). Brown crystals; 30% yield; m.p.: 89.4–92.5°C; Rf 0.59; IR (υ-máx, in cm−1): 1622 (C=N), 1605, 1583, 1496 (C=C), 1324, 1255 (C-N), 1132 (C-O-C); 1H NMR (δ; CDCl3; 300 MHz): 8.2–8.1 (m, 2H, H2′), 7.22–7.1 (m, 1H, H4), 7.0–6.9 (m, 2H, H3′), 6.6 (d, 1H, H6; J = 1.2 Hz), 4.0 (s, 3H, C7-OCH3), 3.8 (s, 3H, C4′-OCH3), 2.6 (t, 2H, CH3-CH2-CH2; J = 7.5 Hz), 1.7–1.6 (m, 2H, CH3-CH2-CH2), 0.9 (t, 3H, CH3-CH2-CH2; J = 7.5 Hz); 13C NMR (δ; CDCl3; 75 MHz): 163.0 (C2), 162.1 (C4′), 144.0 (C7), 143.9 (C3a), 140.0 (C5), 138.0 (C7a), 129.3 (C2′), 119.7 (C1′), 114.2 (C3′), 111.4 (C6), 108.4 (C4), 56.3 (C7-OCH3), 55.4 (C4′-OCH3), 38.4 (CH3-CH2-CH2), 24.9 (CH3-CH2-CH2), 13.7 (CH3-CH2-CH2). HRMS-ESI:m/z calcd for C18H19O3N (M+H)+: 298.1438; found: 298.1450.7-Methoxy-2-(4-nitrophenyl)-5-propylbenzo[d]oxazol (
5d′). Yellow crystals; 33% yield; m.p.: 136.5–140°C; Rf 0.72; IR (υ-máx, in cm−1): 1625 (C=N), 1602 (C=C), 1518, 1339 (NO2), 1309, 1200 (C-N), 1132 (C-O-C); 1H NMR (δ; CDCl3; 300 MHz): 8.4–8.3 (m, 4H, H2′ e H3′), 7.2 (s, 1H, H4), 6.7 (s, 1H, H6), 4.0 (s, 3H, OCH3), 2.7 (t, 2H, CH3-CH2-CH2; J = 7.5 Hz), 1.7–1.6 (m, 2H, CH3-CH2-CH2), 0.9 (t, 3H, CH3-CH2-CH2; J = 7.5 Hz); 13C NMR (δ; CDCl3; 75 MHz): 160.5 (C2), 149.2 (C4′), 144.3 (C7), 143.5 (C3a), 141.0 (C1′), 138.7 (C5), 132.7 (C7a), 128.3 (C2′), 124.1 (C3′), 112.3 (C6), 109.6 (C4), 56.3 (OCH3), 38.4 (CH3-CH2-CH2), 24.8 (CH3-CH2-CH2), 13.7 (CH3-CH2-CH2). HRMS-ESI:m/z calcd for C17H16O4N2 (M+H)+: 313.1183; found: 313.1182.


## 2.2. Antifungal Activity Evaluation

The antifungal activity evaluation was performed through a Mueller-Hinton broth microdilution method and with the methodology and interpretative criteria proposed by document M27A3 [15]. The stock solutions of all the compounds were prepared in DMSO 1% at final concentration and tested at concentrations (µg/mL) 100; 60; 30; 15; 7.5; 3.75; 1.875; 0.468; 0.23; 0.06. The standard drug fluconazole was applied as control of fungistatic action. Results were visualized and analyzed at 530 nm in an Anthos Zenyth 200rt Microplate Reader. The inhibitory concentrations of microbial growth were determined at 50% (IC50) and 90% (IC90) in µmol/mL and compared among the microorganisms. The tests were all done in duplicate.


## 2.3. Cytotoxicity Assay

The cytotoxicity of the compounds (200–1.5μg/mL) to peripheral blood mononuclear cells (PBMCs) was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) method. The PBMCs were obtained from healthy volunteers by Ficoll-Hypaque density gradient centrifugation. These cells were grown in RMPI medium (Cultilab, Brazil) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS) (Cultilab, Brazil), 100 mg/mL penicillin, and 100 mg/mL streptomycin in 5% CO2 atmosphere at 37°C. The cell suspension of PBMCs at a concentration of 2.4 × 106 cells/mL was distributed in a 96-well plate, 90 μL in each well with 10 μL of test compounds at different concentrations, and incubated at 37°C in an incubator at 5% CO2 for 48 h. After that, 10 μL of MTT dye was added (5 mg/mL) and the cells were incubated again for an additional 4 h period. Then, the medium was carefully removed and 100 μL of DMSO was added for solubilization of formazan crystals. The plates were shaken for 5 min, and absorbance for each sample was measured in a spectrophotometric microplate reader at 560 nm. The percentage of cytotoxicity was calculated as [(A-B)/A×100], whereA andB are the absorbance of control and treated cells, respectively. Data were analyzed using linear regression to obtain values for CC50 and CC90 (cytotoxic concentration for 50% and 90% of cells, resp.). Selectivity indexes were expressed as the ratio CC50/IC50. The tests were all done in duplicate.


## 3. Results and Discussion

### 3.1. Chemistry

The synthetic route used to obtain the molecular hybrids is depicted in Scheme1.Scheme 1
Synthetic route to derivatives5a–5d and5a′–5d′. (i) NaNO3, KHSO4, silica/water (1 : 1, w/w), dichloromethane, r.t.; (ii) SnCl2·2H2O, ethanol, 70°C; (iii) benzoyl chlorides, NaHCO3, ethyl ether/water (9 : 1, v/v), 0°C; (iv) PTSA, toluene, reflux.The synthesis of hybrid compounds was accomplished initially byorto-nitration of eugenol (1) and dihydroeugenol (1′) using sodium nitrate, potassium bisulfate, and wet silica gel in dichloromethane, as reported by Zolfigol and coworkers [16]. These nitroderivatives (2 and2′) were obtained in good yields as dark orange oils after successive extractions of the crude material with hexane and solvent evaporation. The next step consisted in reducing these nitrated derivatives to the known aminophenols derivatives3 and3′. This objective was achieved by employing the classical and mild method of reduction of nitrocompounds with stannous chloride in ethanol [17] and the amphoteric intermediates were obtained as pale yellow solids after careful acid-base extractions. The data from spectrometric analysis of intermediates2,2′,3, and3′ showed perfect agreement with those found in the literature [18]. Following, these aminophenols were converted toN-(o-hydroxyphenyl)benzamides by acylation in mild conditions with appropriate carboxylic acid chlorides in an ice-cooled mixture of ether-water and sodium bicarbonate [19]. The benzamides4a–4d and4a′–4d′ were obtained in excellent yields after filtration or solvent extraction. NMR spectra of these intermediates clearly showed the identity of the products and the maintenance of free phenolic hydroxyl, necessary to the next reaction step. Finally, benzoxazoles5a–5d and5a′–5d′ were synthesized by adaptation of the methods described by Nakamura et al. [20] and Yang et al. [21] in which the amides previously obtained were heated under reflux in toluene withp-toluenesulfonic acid as a catalyst and dehydrating agent. The products were obtained in high yields as single products after filtering the reaction mixture through a short pad of silica gel with dichloromethane and removing the solvent in rotavapor. The eight benzoxazoles are new compounds and were fully characterized by IR, NMR, and HRMS analysis.


### 3.2. Biological Evaluations

The final eight compounds were screened for their potential antifungal activity against five fungi species:Candida albicans (C. albicans),C. tropicalis,C. krusei,C. parapsilosis, andC. glabrata (Table 1). The results were calculated using the inhibitory concentration of 50% microbial growth and the interpretative criteria were those proposed by the document M27A3 from the Clinical and Laboratory Standards Institute [15]. Fluconazole was used as the positive control. The assays were performed in triplicate and the results from these replicates were identical.Table 1
In vitro antifungal activity (IC50, μM), cytotoxic activity (CC50, μM), and selectivity index for eugenol (1) and related benzoxazole derivatives (5a–5d′).
Compound
IC50 [μM]∗
CC50 [μM]∗
SI
C. albicans ATCC 10231
C. tropicalis ATCC 750
C. krusei ATCC 6258
C. parapsilosis ATCC 22019
C. glabrata ATCC 90030
1
1524
—
a
1524
—
a
1524
360
0.2
5a
380
—
a
—
a
—
a
—
a
1111
2.9
5a′
—
a
—
a
—
a
—
a
—
a
489
—
b
5b
—
a
—
a
—
a
—
a
—
a
558
—
b
5b′
—
a
—
a
332
—
a
332
629
1.9
5c
331
—
a
—
a
—
a
338
888
2.6
5c′
—
a
—
a
—
a
—
a
—
a
1216
—
b
5d
—
a
—
a
—
a
—
a
—
a
1208
—
b
5d′
321
—
a
321
—
a
—
a
613
1.9
FLC
1.6
3.3
104.3
3.3
52.2
—
b
—
b
∗The experiments were done in triplicate and the results were identical; ano significant activity; bnot determined; SI: selectivity indexes, expressed as the ratio CC50/IC50; FLC: fluconazole.Our expectation was that the fusion of two pharmacophores, that is, eugenol and benzoxazole ring, would enhance eugenol antimicrobial profiles against those fungi. In fact, four products showed fungistatic properties in the range 321–380μM. Moreover, it is interesting to note that these derivatives were about five times more potent than eugenol, which in turn could only inhibit three strains growing up to the highest concentration used. Benzoxazoles5a,5c, and5d′ showed good fungistatic activities againstC. albicans (IC50 values of 380, 331, and 321 μM, resp.), while compounds5b′ and5c could also inhibitC. glabrata growing at 332 and 338 μM, respectively. Derivatives5b′ and5d′ inhibited alsoC. krusei growing at IC50 332 and 321 μM, sequentially. Notably, this shows that the potential of this new kind of derivative against this strain sinceC. krusei is naturally resistant to many azole drugs, for example, fluconazole [22]. Our study corroborates the findings of Carrasco et al. [23], which showed that the phenolic hydroxyl was not necessary in antifungal action againstCandida sp. An influence of the substituent group on thepara-position of phenyl ring was observed for products5c and5d since the presence of the electron-donating group in5c led to an activated benzoxazole, while the nitrosubstituted product5dis among the inactive compounds. However, it is not possible to use this observation as a rule, since, in propyl derivatives5c′ and5d′, one can see the opposite.The selective toxicity of these benzoxazoles, expressed as the selectivity index (SI), was then assessed on peripheral human blood mononuclear cells. This parameter reflects the quantity of compound that is active against the pathogen but is not toxic towards the host cell. Therefore, compounds with a higher SI are very promising because the concentration of the compound to induce an antimicrobial activity is lower than the dose that induces cytotoxicity in host cells. The analysis of SI values is very important to establish if the chemical modifications in each of the compounds can increase the antifungal activity and decrease the cytotoxicity to host cells.The results from cytotoxicity assays showed that all hybrids had higher CC50 values than eugenol (Table 1). Compounds5a,5b′,5c, and5d′ presented selectivity index (SI) values 9 to 15 times higher than eugenol. The blockade of the phenolic hydroxyl may be related to the decreased toxicity of eugenol in mammalian cells, while the nature ofpara-substituents in phenyl ring showed no clear relationship with the observed cytotoxicity profile, as noted in the results of antifungal evaluation. Meantime, it could be noted that derivatives5aand5d were less toxic than their propyl analogs5a′ and5d′. In vivo, the allyl group can lead to toxic metabolic products [24], but it is not always a toxicophore, since this will depend on other structural factors associated with the whole molecule.In short, these are new antifungal prototypes, which may be used for future chemical modifications to improve activity and reduce cytotoxicity.


## 3.1. Chemistry

The synthetic route used to obtain the molecular hybrids is depicted in Scheme1.Scheme 1
Synthetic route to derivatives5a–5d and5a′–5d′. (i) NaNO3, KHSO4, silica/water (1 : 1, w/w), dichloromethane, r.t.; (ii) SnCl2·2H2O, ethanol, 70°C; (iii) benzoyl chlorides, NaHCO3, ethyl ether/water (9 : 1, v/v), 0°C; (iv) PTSA, toluene, reflux.The synthesis of hybrid compounds was accomplished initially byorto-nitration of eugenol (1) and dihydroeugenol (1′) using sodium nitrate, potassium bisulfate, and wet silica gel in dichloromethane, as reported by Zolfigol and coworkers [16]. These nitroderivatives (2 and2′) were obtained in good yields as dark orange oils after successive extractions of the crude material with hexane and solvent evaporation. The next step consisted in reducing these nitrated derivatives to the known aminophenols derivatives3 and3′. This objective was achieved by employing the classical and mild method of reduction of nitrocompounds with stannous chloride in ethanol [17] and the amphoteric intermediates were obtained as pale yellow solids after careful acid-base extractions. The data from spectrometric analysis of intermediates2,2′,3, and3′ showed perfect agreement with those found in the literature [18]. Following, these aminophenols were converted toN-(o-hydroxyphenyl)benzamides by acylation in mild conditions with appropriate carboxylic acid chlorides in an ice-cooled mixture of ether-water and sodium bicarbonate [19]. The benzamides4a–4d and4a′–4d′ were obtained in excellent yields after filtration or solvent extraction. NMR spectra of these intermediates clearly showed the identity of the products and the maintenance of free phenolic hydroxyl, necessary to the next reaction step. Finally, benzoxazoles5a–5d and5a′–5d′ were synthesized by adaptation of the methods described by Nakamura et al. [20] and Yang et al. [21] in which the amides previously obtained were heated under reflux in toluene withp-toluenesulfonic acid as a catalyst and dehydrating agent. The products were obtained in high yields as single products after filtering the reaction mixture through a short pad of silica gel with dichloromethane and removing the solvent in rotavapor. The eight benzoxazoles are new compounds and were fully characterized by IR, NMR, and HRMS analysis.


## 3.2. Biological Evaluations

The final eight compounds were screened for their potential antifungal activity against five fungi species:Candida albicans (C. albicans),C. tropicalis,C. krusei,C. parapsilosis, andC. glabrata (Table 1). The results were calculated using the inhibitory concentration of 50% microbial growth and the interpretative criteria were those proposed by the document M27A3 from the Clinical and Laboratory Standards Institute [15]. Fluconazole was used as the positive control. The assays were performed in triplicate and the results from these replicates were identical.Table 1
In vitro antifungal activity (IC50, μM), cytotoxic activity (CC50, μM), and selectivity index for eugenol (1) and related benzoxazole derivatives (5a–5d′).
Compound
IC50 [μM]∗
CC50 [μM]∗
SI
C. albicans ATCC 10231
C. tropicalis ATCC 750
C. krusei ATCC 6258
C. parapsilosis ATCC 22019
C. glabrata ATCC 90030
1
1524
—
a
1524
—
a
1524
360
0.2
5a
380
—
a
—
a
—
a
—
a
1111
2.9
5a′
—
a
—
a
—
a
—
a
—
a
489
—
b
5b
—
a
—
a
—
a
—
a
—
a
558
—
b
5b′
—
a
—
a
332
—
a
332
629
1.9
5c
331
—
a
—
a
—
a
338
888
2.6
5c′
—
a
—
a
—
a
—
a
—
a
1216
—
b
5d
—
a
—
a
—
a
—
a
—
a
1208
—
b
5d′
321
—
a
321
—
a
—
a
613
1.9
FLC
1.6
3.3
104.3
3.3
52.2
—
b
—
b
∗The experiments were done in triplicate and the results were identical; ano significant activity; bnot determined; SI: selectivity indexes, expressed as the ratio CC50/IC50; FLC: fluconazole.Our expectation was that the fusion of two pharmacophores, that is, eugenol and benzoxazole ring, would enhance eugenol antimicrobial profiles against those fungi. In fact, four products showed fungistatic properties in the range 321–380μM. Moreover, it is interesting to note that these derivatives were about five times more potent than eugenol, which in turn could only inhibit three strains growing up to the highest concentration used. Benzoxazoles5a,5c, and5d′ showed good fungistatic activities againstC. albicans (IC50 values of 380, 331, and 321 μM, resp.), while compounds5b′ and5c could also inhibitC. glabrata growing at 332 and 338 μM, respectively. Derivatives5b′ and5d′ inhibited alsoC. krusei growing at IC50 332 and 321 μM, sequentially. Notably, this shows that the potential of this new kind of derivative against this strain sinceC. krusei is naturally resistant to many azole drugs, for example, fluconazole [22]. Our study corroborates the findings of Carrasco et al. [23], which showed that the phenolic hydroxyl was not necessary in antifungal action againstCandida sp. An influence of the substituent group on thepara-position of phenyl ring was observed for products5c and5d since the presence of the electron-donating group in5c led to an activated benzoxazole, while the nitrosubstituted product5dis among the inactive compounds. However, it is not possible to use this observation as a rule, since, in propyl derivatives5c′ and5d′, one can see the opposite.The selective toxicity of these benzoxazoles, expressed as the selectivity index (SI), was then assessed on peripheral human blood mononuclear cells. This parameter reflects the quantity of compound that is active against the pathogen but is not toxic towards the host cell. Therefore, compounds with a higher SI are very promising because the concentration of the compound to induce an antimicrobial activity is lower than the dose that induces cytotoxicity in host cells. The analysis of SI values is very important to establish if the chemical modifications in each of the compounds can increase the antifungal activity and decrease the cytotoxicity to host cells.The results from cytotoxicity assays showed that all hybrids had higher CC50 values than eugenol (Table 1). Compounds5a,5b′,5c, and5d′ presented selectivity index (SI) values 9 to 15 times higher than eugenol. The blockade of the phenolic hydroxyl may be related to the decreased toxicity of eugenol in mammalian cells, while the nature ofpara-substituents in phenyl ring showed no clear relationship with the observed cytotoxicity profile, as noted in the results of antifungal evaluation. Meantime, it could be noted that derivatives5aand5d were less toxic than their propyl analogs5a′ and5d′. In vivo, the allyl group can lead to toxic metabolic products [24], but it is not always a toxicophore, since this will depend on other structural factors associated with the whole molecule.In short, these are new antifungal prototypes, which may be used for future chemical modifications to improve activity and reduce cytotoxicity.


## 4. Conclusion

The present study described the synthesis and antifungal evaluation of benzoxazoles devised by molecular hybridization with eugenol or dihydroeugenol. Four of them (5a,5b′,5c, and5c′) showed activity against pathogenic and opportunistic species ofCandida spp. against which eugenol had only modest activity. Furthermore, they were 9–15 times more selective than eugenol in cytotoxicity test. The ongoing investigation of the mechanism by which these substances play antifungal effects can lead to a new class of antimicrobial candidates and these benzoxazoles can be submitted to structural variation for optimization of antifungal activity.



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*Source: 5207439-2017-07-24.xml*