# Pathological Scenario with the Mannose-Binding Lectin in Patients with IgA Nephropathy

Authors: Isao Ohsawa; Masaya Ishii; Hiroyuki Ohi; Yasuhiko Tomino
Journal: Journal of Biomedicine and Biotechnology
 (2012)

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## Abstract

A deeper understanding of the mechanism of complement activation may help to elucidate the pathogenesis of IgA nephropathy (IgAN). Traditionally, the activation of an alternative pathway (AP) has been recognized as an enhancer mechanism of glomerular damage. This paper documents contemporary information concerning the possible pathological mechanisms of the lectin pathway (LP) in the circulation and in the glomerulus. The circulating initiator of LP activation is not fully understood. However, ligands for mannose-binding lectin (MBL) which are among the starter molecules of the LP are aberrant glycosylated molecules-containing immune complex.  Recent reports have focused onN-glycans on secretory IgA as a candidate ligand. Mesangial deposits of MBL are seen in 25% of patients with IgAN. Mesangial deposits of MBL and C4 and/or C4 breakdown products are implicated as markers for disease progression of IgAN. On the other hand, patients with MBL deficiency tend to show better clinical presentation and lower levels of urinary protein and serum creatinine than MBL-sufficient patients. It is now recognized that involvement of  AP and  LP constitutes an additional mechanism for explaining the progression of IgAN.


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## Body

## 1. Introduction

IgA nephropathy (IgAN) is the most common form of primary glomerulonephritis. It is generally agreed that up to 30% of patients with IgAN eventually progress to end-stage kidney disease [1]. The diagnosis of IgAN can be confirmed only by renal biopsy that shows mesangial cell proliferation and mesangial IgA deposits. In the circulation, serum levels of IgA are increased in approximately 50% of patients with IgAN. Recently, the key feature has been considered to be the deficiency of galactose in the hinge region of the IgA1 heavy chains and it is thought that these abnormal IgA1 glycosylations reduce clearance of immune complexes which may then be sufficient to trigger glomerular mesangial deposition [2].With respect to complement deposition, C3 is highly detected in glomeruli and the detection rate is found in more than 90% of patients of IgAN [3, 4]. Since other complement molecules, such as properdin and C5, are colocated in the mesangial areas with IgA, but C1q is absent and there is an increase of C3 breakdown products in the serum of patients with IgAN, we had recognized that alternative pathway (AP) activation is involved in the pathogenesis of IgAN [5]. After discovering new molecules which belong to the lectin pathway (LP), we came to believe there is accumulating evidence to support a hypothesis of the occurrence of LP activation in the pathogenesis of IgAN. The issues surrounding these scenarios with LP activation are reviewed to summarize their potential significance in the pathogenesis of IgAN. In order to explain the updated knowledge in plain terms, the main body was separated into three parts, “In circulation,” “ In kidney,” and “Infection and clinical course.”


## 2. In Circulation

### 2.1. Serum Levels of MBL in Patients with IgA Nephropathy

We previously measured the serum concentrations of MBL in 20 healthy controls and in 80 patients with primary glomerular disease (Figure1). The serum concentrations of MBL from the patients presented a wider distribution than in healthy controls, but a comparison of the mean levels from each group showed no significant difference. Since MBL levels are altered by more than 3-4 times baseline levels during severe infection [6], production of MBL might be enhanced under some context, such as inflammation. Our colleague, Onda, had reported that the patients with IgAN showed hypercomplementemia; the mean of CH50 titer and serum levels of C4, factor B and properdin in patients with IgAN were significantly higher than those in normal controls (Table 1) [7]. Although hypercomplementemia is derived from chronic inflammation which accelerates the production of complement components in IgAN, it is interesting to note that serum concentrations of C3 and MBL were relatively lower than in normal controls. Lhotta et al. [8] and Roos et al. [9] also previously described no significant difference of mean levels of serum MBL between healthy controls and IgAN patients. This implies that consumption of C3 may increase by LP activation, thereby adding to AP activation.Table 1
Serum levels of complement components in patients with IgA nephropathy [7].
nCH50C1qC4C3C5BPMBL(U/mL)(mg/dL)(mg/dL)(mg/dL)(%)(%)(mg/mL)(mg/mL)IgA nephropathy5044.0 ± 8.1*13.4 ± 2.828 ± 11*101 ± 26122 ± 28114 ± 32*32.6 ± 27.0*1.8 ± 1.8Healthy controls5033.5 ± 5.412.6 ± 1.721 ± 5106 ± 17112 ± 1795 ± 1821.0 ± 24.02.1 ± 1.8% expresses a percentage of pooled normal human serum, *:P<0.01 IgA nephropathy versus healthy controls.Figure 1
The serum concentrations of MBL were measured in 20 healthy controls and in 80 patients of primary glomerular diseases. The mean levels of MBL showed no statistical difference between each disease. However, some cases of primary glomerulonephritis presented higher concentrations of MBL compared with healthy controls. Abbreviations. MCNS, minimal change nephrotic syndrome (n=16); IgAN, IgA nephropathy (n=19); MPGN, membranoproliferative glomerulonephritis (n=31); others (n=14).


### 2.2. Initiators of Lectin Pathway Activation

From the 1980s, researchers have explored the essential molecules in the pathogenesis of IgAN. Tomino et al. deduced by an immunohistochemistry technique that the mesangial deposition of IgA1 is critical for glomerular damage, and a subsequent study showed that the polymeric form of IgA1 is predominantly eluted from renal tissues of IgAN patients [10, 11]. Conley et al. also confirmed a more predominantly glomerular deposition of IgA1 than that of IgA2 [12]. Recently, unique O-linked glycosylation sites at the hinge region of IgA1 have received much attention. From the analysis of the circulating immune complex, the galactose deficiency (under-O-glycosylated IgA) has been pointed out in the IgA1 molecules of patients with IgAN [13–15], although a high circulating overload alone does not induce the glomerular damage. As demonstrated in the current reviews, synthesis and binding of autoantibodies directed against galactose-deficient IgA1 (GalNAc residues on the hinge region) are required for the formation of pathogenic IgA1-containing immune complexes [2, 16]. Excesses of these relatively large complexes might lodge into the paramesangial area and might activate LP.Since under-O-glycosylated IgA leads to polymerization of IgA [17] and purified polymeric IgA from patients with IgAN can activate LP [18], an important question is whether aberrant IgA can activate LP. Prominent ligands for MBL are mannose and N-acetyl-glucosamine (GlcNAc), and ligands for ficolins are GlcNAc. Because galactose and sialic acid are usually present in mammalian glycoproteins, they cannot be captured by MBL and ficolins [19]. Although such an under-O-glycosylated IgA1 from patients with IgAN could potentially interact with plant lectins [20], MBL and ficolins have no lectin activity for GalNAc residues. To the best of our knowledge, galactose-deficient IgA1 itself is able to activate C3 directly [9] but no direct clinical evidence from human studies is available which has demonstrated that MBL and ficolins can bind to aberrant IgA1 in human studies.


### 2.3. Other Candidate Ligands of MBL

Secretory IgA (SIgA) is the first line of defense in protecting the respiratory and intestinal tract, and the serum concentration of SIgA is very low (2-3μg/mL). Since the N-glycans on the heavy chains of both SIgA1 and SIgA2 present terminal GlcNAc and mannose residues, they could be recognized by MBL and ficolins [21]. Oortwijn et al. clearly demonstrated glomerular staining of SIgA with MBL and C4d in the patients with IgAN [22]. Further extended study is needed to clarify the pathological role of SIgA and complement activation.


### 2.4. MBL Deficiency

Functional MBL deficiency is probably the most common immunodeficiency in humans, involving 10 to 20% of the population. It is characterized by low levels of circulating functional multimers due to a number of genetic polymorphisms within the coding (codon 54 of exon 1 is most common in humans and it determines serum concentration and carbohydrate recognition ability) and promoter regions of theMBL2gene [23]. Our preliminary comparisons of the clinical backgrounds of MBL-sufficient patients of IgAN and MBL deficient patients of IgAN were determined by the analysis of polymorphisms of codon 54 (Table 2). The means of urinary protein and glomerular filtration rate (GFR) in MBL deficient subjects were better than in MBL-sufficient subjects, but lacked statistical significance. Pirulli et al. showed that polymorphism of codon 54 did not appear to have a primary involvement in the susceptibility and severity of IgAN in Italian patients [24]. They also reported a similar distribution of polymorphism frequency between healthy volunteers and IgAN patients. Nevertheless, the Pirulli’s data and ours could have been influenced by the low number of IgAN patients and this might be due to a racial difference. Of great interest is whether the difference can be found between MBL deficiency and MBL sufficiency at the onset and progression of IgAN.Table 2
Clinical background compared with MBL sufficient and MBL deficient of IgA nephropathy.
MBL sufficient(n=55)MBL deficient(n=6)Gender (M : F)22 : 333 : 3Age (y)30.6 ± 8.726.5 ± 6.7Serum creatinine (mg/dL)0.83 ± 0.300.71 ± 0.14Estimated GFR (mL/min/1.73 m2)85.3 ± 30.998.4 ± 13.8Urinary protein (g/g·creatinine)1.45 ± 1.580.69 ± 0.97History of macrohematuria (%)27.333.3IgA (mg/dL)316.9 ± 112.9265.2 ± 69.6C3 (mg/dL)97.8 ± 15.893.2 ± 2.6C4 (mg/dL)21.9 ± 6.119.8 ± 6.6CH50 (Unit/mL)40.1 ± 6.734.4 ± 3.4


## 2.1. Serum Levels of MBL in Patients with IgA Nephropathy

We previously measured the serum concentrations of MBL in 20 healthy controls and in 80 patients with primary glomerular disease (Figure1). The serum concentrations of MBL from the patients presented a wider distribution than in healthy controls, but a comparison of the mean levels from each group showed no significant difference. Since MBL levels are altered by more than 3-4 times baseline levels during severe infection [6], production of MBL might be enhanced under some context, such as inflammation. Our colleague, Onda, had reported that the patients with IgAN showed hypercomplementemia; the mean of CH50 titer and serum levels of C4, factor B and properdin in patients with IgAN were significantly higher than those in normal controls (Table 1) [7]. Although hypercomplementemia is derived from chronic inflammation which accelerates the production of complement components in IgAN, it is interesting to note that serum concentrations of C3 and MBL were relatively lower than in normal controls. Lhotta et al. [8] and Roos et al. [9] also previously described no significant difference of mean levels of serum MBL between healthy controls and IgAN patients. This implies that consumption of C3 may increase by LP activation, thereby adding to AP activation.Table 1
Serum levels of complement components in patients with IgA nephropathy [7].
nCH50C1qC4C3C5BPMBL(U/mL)(mg/dL)(mg/dL)(mg/dL)(%)(%)(mg/mL)(mg/mL)IgA nephropathy5044.0 ± 8.1*13.4 ± 2.828 ± 11*101 ± 26122 ± 28114 ± 32*32.6 ± 27.0*1.8 ± 1.8Healthy controls5033.5 ± 5.412.6 ± 1.721 ± 5106 ± 17112 ± 1795 ± 1821.0 ± 24.02.1 ± 1.8% expresses a percentage of pooled normal human serum, *:P<0.01 IgA nephropathy versus healthy controls.Figure 1
The serum concentrations of MBL were measured in 20 healthy controls and in 80 patients of primary glomerular diseases. The mean levels of MBL showed no statistical difference between each disease. However, some cases of primary glomerulonephritis presented higher concentrations of MBL compared with healthy controls. Abbreviations. MCNS, minimal change nephrotic syndrome (n=16); IgAN, IgA nephropathy (n=19); MPGN, membranoproliferative glomerulonephritis (n=31); others (n=14).


## 2.2. Initiators of Lectin Pathway Activation

From the 1980s, researchers have explored the essential molecules in the pathogenesis of IgAN. Tomino et al. deduced by an immunohistochemistry technique that the mesangial deposition of IgA1 is critical for glomerular damage, and a subsequent study showed that the polymeric form of IgA1 is predominantly eluted from renal tissues of IgAN patients [10, 11]. Conley et al. also confirmed a more predominantly glomerular deposition of IgA1 than that of IgA2 [12]. Recently, unique O-linked glycosylation sites at the hinge region of IgA1 have received much attention. From the analysis of the circulating immune complex, the galactose deficiency (under-O-glycosylated IgA) has been pointed out in the IgA1 molecules of patients with IgAN [13–15], although a high circulating overload alone does not induce the glomerular damage. As demonstrated in the current reviews, synthesis and binding of autoantibodies directed against galactose-deficient IgA1 (GalNAc residues on the hinge region) are required for the formation of pathogenic IgA1-containing immune complexes [2, 16]. Excesses of these relatively large complexes might lodge into the paramesangial area and might activate LP.Since under-O-glycosylated IgA leads to polymerization of IgA [17] and purified polymeric IgA from patients with IgAN can activate LP [18], an important question is whether aberrant IgA can activate LP. Prominent ligands for MBL are mannose and N-acetyl-glucosamine (GlcNAc), and ligands for ficolins are GlcNAc. Because galactose and sialic acid are usually present in mammalian glycoproteins, they cannot be captured by MBL and ficolins [19]. Although such an under-O-glycosylated IgA1 from patients with IgAN could potentially interact with plant lectins [20], MBL and ficolins have no lectin activity for GalNAc residues. To the best of our knowledge, galactose-deficient IgA1 itself is able to activate C3 directly [9] but no direct clinical evidence from human studies is available which has demonstrated that MBL and ficolins can bind to aberrant IgA1 in human studies.


## 2.3. Other Candidate Ligands of MBL

Secretory IgA (SIgA) is the first line of defense in protecting the respiratory and intestinal tract, and the serum concentration of SIgA is very low (2-3μg/mL). Since the N-glycans on the heavy chains of both SIgA1 and SIgA2 present terminal GlcNAc and mannose residues, they could be recognized by MBL and ficolins [21]. Oortwijn et al. clearly demonstrated glomerular staining of SIgA with MBL and C4d in the patients with IgAN [22]. Further extended study is needed to clarify the pathological role of SIgA and complement activation.


## 2.4. MBL Deficiency

Functional MBL deficiency is probably the most common immunodeficiency in humans, involving 10 to 20% of the population. It is characterized by low levels of circulating functional multimers due to a number of genetic polymorphisms within the coding (codon 54 of exon 1 is most common in humans and it determines serum concentration and carbohydrate recognition ability) and promoter regions of theMBL2gene [23]. Our preliminary comparisons of the clinical backgrounds of MBL-sufficient patients of IgAN and MBL deficient patients of IgAN were determined by the analysis of polymorphisms of codon 54 (Table 2). The means of urinary protein and glomerular filtration rate (GFR) in MBL deficient subjects were better than in MBL-sufficient subjects, but lacked statistical significance. Pirulli et al. showed that polymorphism of codon 54 did not appear to have a primary involvement in the susceptibility and severity of IgAN in Italian patients [24]. They also reported a similar distribution of polymorphism frequency between healthy volunteers and IgAN patients. Nevertheless, the Pirulli’s data and ours could have been influenced by the low number of IgAN patients and this might be due to a racial difference. Of great interest is whether the difference can be found between MBL deficiency and MBL sufficiency at the onset and progression of IgAN.Table 2
Clinical background compared with MBL sufficient and MBL deficient of IgA nephropathy.
MBL sufficient(n=55)MBL deficient(n=6)Gender (M : F)22 : 333 : 3Age (y)30.6 ± 8.726.5 ± 6.7Serum creatinine (mg/dL)0.83 ± 0.300.71 ± 0.14Estimated GFR (mL/min/1.73 m2)85.3 ± 30.998.4 ± 13.8Urinary protein (g/g·creatinine)1.45 ± 1.580.69 ± 0.97History of macrohematuria (%)27.333.3IgA (mg/dL)316.9 ± 112.9265.2 ± 69.6C3 (mg/dL)97.8 ± 15.893.2 ± 2.6C4 (mg/dL)21.9 ± 6.119.8 ± 6.6CH50 (Unit/mL)40.1 ± 6.734.4 ± 3.4


## 3. In Kidney

### 3.1. Evidence of Glomerular Deposition of Mannose-Binding Lectin

Endo et al. first demonstrated that glomerular deposition of MBL/MASP-1 occurred in in the 25% of the cases of IgAN and that this was not observed in normal kidney and that the frequency was higher than that found in the other forms of glomerulonephritis [25]. In a subsequent paper, Lhotta et al. also confirmed that 27% of patients with IgAN had mesangial deposits of MBL [8]. Furthermore, the recent study by Roos et al. reported that the most MBL-positive patients presented mesangial deposits of L-ficolin and IgA1 but not IgA2 [9]. These reports suggested that MBL binds to immune complexes which contain aberrant IgA and/or IgG. Contrary to the above reports, Hisano et al. showed that patients with mesangial deposits of IgA1 and C3c showed no deposits of C4, MBL, and MASP-1. Moreover, patients with mesangial deposits of IgA1, IgA2, and C3c showed deposition of C4, MBL, and MASP-1. Furthermore, C1q deposition was not found in any patient. They concluded that AP activation occurs as a result of mesangial deposition of IgA1 and that LP activation is associated with mesangial deposition of IgA2 [26]. It is clear, however, that a determination of the initiator of LP activation in the mesangium has not yet been carried out to complete satisfaction.In IgAN, the protease-damaged surface of glomerular resident cells or apoptotic cells can be recognized by MBL. In regard to patients of lupus nephritis, we recently proposed that positive glomerular staining for annexin V would be seen in the majority of patients who had confirmed glomerular deposits of MBL, L-ficolin, and properdin [27]. Further examinations will also be necessary to elucidate the initial activator of LP in situ.


### 3.2. Clinical Significance of Lectin Pathway Activation in Glomeruli

Endo et al. reported that the patients with glomerular deposits of MBL/MASP-1 deposition were young and that the duration of the disease prior to renal biopsy was short compared with that in patients without MBL/MASP-1 deposition [25]. In considering histological alterations, patients with glomerular deposits of MBL/MASP-1 have a more distinct activity index than chronicity index. Roos et al. compared IgAN patients who had negative glomerular staining for MBL with those who had positive staining for MBL [9]. They concluded that the activation of the LP was associated with more severe renal damage, as demonstrated by proteinuria, decreased renal function, and more severe histological findings, such as mesangial proliferation, crescent formation, glomerular sclerosis, and interstitial fibrosis. Accordingly, it is possible to envisage a scenario in which glomerular activation of LP might play an enhancer of progression of glomerular damage. Thus, we should perform immunofluorescent analysis of C4 and/or C4 breakdown products (C4c, C4d), and this should be a routine procedure in histological work and the presence of mesangial deposits of C4 may help us to identify patients with worse prognosis [28].Contrary to the studies described above, Hisano et al. showed that there were no differences in clinical and pathological severity parameters for the patients with mesangial deposits of MBL [26]. In their paper, patients with mesangial deposits of MBL and IgA2 constituted more than half of the patients examined, which was a higher proportion than those described in the reports of Endo et al. and Roos et al. Because IgAN is chronic disease of long duration and shows a broad spectrum of clinical presentations, it is difficult to plan to compare their clinical course. At any rate, LP activation in the glomerulus can be identified in the context of histological examinations.


## 3.1. Evidence of Glomerular Deposition of Mannose-Binding Lectin

Endo et al. first demonstrated that glomerular deposition of MBL/MASP-1 occurred in in the 25% of the cases of IgAN and that this was not observed in normal kidney and that the frequency was higher than that found in the other forms of glomerulonephritis [25]. In a subsequent paper, Lhotta et al. also confirmed that 27% of patients with IgAN had mesangial deposits of MBL [8]. Furthermore, the recent study by Roos et al. reported that the most MBL-positive patients presented mesangial deposits of L-ficolin and IgA1 but not IgA2 [9]. These reports suggested that MBL binds to immune complexes which contain aberrant IgA and/or IgG. Contrary to the above reports, Hisano et al. showed that patients with mesangial deposits of IgA1 and C3c showed no deposits of C4, MBL, and MASP-1. Moreover, patients with mesangial deposits of IgA1, IgA2, and C3c showed deposition of C4, MBL, and MASP-1. Furthermore, C1q deposition was not found in any patient. They concluded that AP activation occurs as a result of mesangial deposition of IgA1 and that LP activation is associated with mesangial deposition of IgA2 [26]. It is clear, however, that a determination of the initiator of LP activation in the mesangium has not yet been carried out to complete satisfaction.In IgAN, the protease-damaged surface of glomerular resident cells or apoptotic cells can be recognized by MBL. In regard to patients of lupus nephritis, we recently proposed that positive glomerular staining for annexin V would be seen in the majority of patients who had confirmed glomerular deposits of MBL, L-ficolin, and properdin [27]. Further examinations will also be necessary to elucidate the initial activator of LP in situ.


## 3.2. Clinical Significance of Lectin Pathway Activation in Glomeruli

Endo et al. reported that the patients with glomerular deposits of MBL/MASP-1 deposition were young and that the duration of the disease prior to renal biopsy was short compared with that in patients without MBL/MASP-1 deposition [25]. In considering histological alterations, patients with glomerular deposits of MBL/MASP-1 have a more distinct activity index than chronicity index. Roos et al. compared IgAN patients who had negative glomerular staining for MBL with those who had positive staining for MBL [9]. They concluded that the activation of the LP was associated with more severe renal damage, as demonstrated by proteinuria, decreased renal function, and more severe histological findings, such as mesangial proliferation, crescent formation, glomerular sclerosis, and interstitial fibrosis. Accordingly, it is possible to envisage a scenario in which glomerular activation of LP might play an enhancer of progression of glomerular damage. Thus, we should perform immunofluorescent analysis of C4 and/or C4 breakdown products (C4c, C4d), and this should be a routine procedure in histological work and the presence of mesangial deposits of C4 may help us to identify patients with worse prognosis [28].Contrary to the studies described above, Hisano et al. showed that there were no differences in clinical and pathological severity parameters for the patients with mesangial deposits of MBL [26]. In their paper, patients with mesangial deposits of MBL and IgA2 constituted more than half of the patients examined, which was a higher proportion than those described in the reports of Endo et al. and Roos et al. Because IgAN is chronic disease of long duration and shows a broad spectrum of clinical presentations, it is difficult to plan to compare their clinical course. At any rate, LP activation in the glomerulus can be identified in the context of histological examinations.


## 4. Infection and Clinical Course of IgA Nephropathy

Previous reports had indicated that glomerular deposits of MBL were present in the patients of postinfectious diseases and that causative pathogens were variable, such asStreptococcus [29, 30], Propionibacterium [31], Mucor [32], and hepatitis C virus [33]. In IgAN, several pathogenic viral and bacterial antigens have been proposed as being responsible for the formation of mesangial deposits of IgA and infection might trigger the onset and progression of the diseases [34, 35]. Up to now, there has benn only one report which has explored the association between MBL polymorphism and clinical course of IgAN. Gong et al. described the clinical significance of MBL polymorphism of codon 54 in patients with IgAN [36]. In this report, the patients carrying the variant allele (GAC) had episodes of upper respiratory or gastrointestinal infections prior to the onset, or exacerbation of IgAN which are absent in wild homozygotes (GGC/GGC). The association between infection and progression of glomerular damage via LP activation is a promising area of research for obtaining a better understanding of the pathogenesis of IgAN.


## 5. Conclusion

It appears that the activations of AP and LP are very much involved in the pathogenesis of IgAN. The traces of LP activation in the fluid phase and local glomerular tissues have been shown to accumulate in a subpopulation of IgAN. However, further studies will be essential to confirm the ligands for the MBL in the pathogenesis of IgAN.



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