# Impact of Forage Fertilization with Urea and Composted Cattle Manure on Soil Fertility in Sandy Soils of South-Central Vietnam

Authors: Keenan C. McRoberts; Quirine M. Ketterings; David Parsons; Tran Thanh Hai; Nguyen Hai Quan; Nguyen Xuan Ba; Charles F. Nicholson; Debbie J. R.  Cherney
Journal: International Journal of Agronomy
 (2016)

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## Abstract

Increased production in smallholder beef systems requires improved forage management. Our objective was to evaluate the effects of composted cattle manure and mineral nitrogen (urea) application on soil fertility and partial nutrient balances in plots established toBrachiaria cv. Mulato II in south-central coastal Vietnam from 2010 to 2013. A randomized complete block design was implemented on six farms (blocks), with five rates of composted cattle manure (0, 4, 8, 12, and 24 Mg DM/ha per yr) and three urea rates (0, 60, and 120 kg N/ha per yr) in a factorial design. Soil was analyzed before and after the experiment. Compost increased soil pH, organic matter, Ca, Mg, and Mn. The effect of compost and urea applications on postexperiment soil fertility depended on preexperiment soil fertility for K, P, S, Mg, Zn, Mn, Cu, and organic matter, suggesting that the ability to maintain soil fertility depends on the interaction between soil organic and inorganic amendments and existing soil fertility. Highest farm yields were also achieved on farms with higher preexperiment soil fertility levels. Negative partial nutrient balances for N, P, and K suggest that yields will not be sustainable over time even for the highest fertilization inputs used in this experiment.


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## Body

## 1. Introduction

Smallholder crop-livestock farms (≤5 head of cattle/household) in south-central coastal Vietnam contribute to beef supply for urban areas [1]. Semi-intensive (grazing and stall feeding) and extensive (grazing of communal land) cattle management systems are dominant in the region. Supplementary stall feeding, with a basal diet of rice straw, peanut straw, and cultivated forage (e.g.,Pennisetum purpureum) or cut-and-carry native grasses and legumes from private and communal land, complements supervised or unsupervised grazing on communal (and private) lands [1]. Progressive farmers have transitioned toward semi-intensive management of backyard plots to increase animal productivity (daily gain) using supplementary forages (native grasses and legumes and cultivated improved forages) and concentrates and reducing energy expenditures incurred from walking to open grazing areas. Key to this transition is cultivation of high-yielding, high nutritive value forages that are well adapted to local climatic and soil conditions [1–4]. Farmers also benefit from reduced labor requirements for cattle production when forages are cultivated near the household [3, 5].Adoption ofBrachiaria spp. grasses in tropical Latin America has been important to productivity and economic gains in dairy and beef systems [6] and can be managed under grazing or cut-and-carry systems. Similar benefits are emerging in smallholder crop-livestock systems in Vietnam and other southeast Asian countries due to adoption ofBrachiaria spp. hybrids such as Mulato (B. brizantha × B. ruziziensis) and Mulato II (B. ruziziensis ×B. decumbens ×B. brizantha) and other improved forages [2, 4, 7].Brachiaria spp. hybrids are popular among farmers in Vietnam due to high palatability, high leaf to stem ratios, easy establishment, and good yield potential relative to other forages [2, 4].Sandy soils in Vietnam, similar to other tropical systems, pose a unique set of fertility constraints [8], including nutrient deficiencies and low soil organic matter (OM) concentrations [9]. Low organic matter contributes to low cation exchange capacity (CEC), low water holding capacity, and high potential nutrient losses due to runoff, leaching, volatilization, and denitrification. Data supporting forage management decisions in sandy, tropical, rain-fed systems such as those located in the south-central Vietnam coast are inadequate [7, 10]. Vietnamese farmers identify fertilization management of forages as a critical decision impacting productivity and soil fertility over time. Palm et al. [11], Zingore et al. [12], Goyal et al. [13], and Kaur et al. [14] suggest that a combination of locally available organic nutrient sources and fertilizer might be needed to enhance productivity in the tropics. Farmers in south-central coastal Vietnam typically use composted cattle manure combined with occasional inorganic fertilizer applications (urea and/or NPK blends) and generally follow a “more is better” approach to composted manure applications based on plant color and vigor.The objective of this study was to assess the effects of urea and composted cattle manure on soil fertility and partial nutrient balances in a multiyear (29 mo) on-farm field experiment. We report on soil fertility and nutrient balances under various nutrient management scenarios.


## 2. Methods

### 2.1. Cát Trinh Commune Characteristics

The experiment was undertaken from 2010 to 2013 in Cát Trinh Commune, Phù Cát District, Bình Định Province in south-central coastal Vietnam. The district capital of Ngô Mây (14°0′2′′N, 109°2′38′′E) borders Cát Trinh Commune to the southwest. Sandy soils in the commune belong to the Arenosols Group [21]. Annual rainfall in the region is about 1,200 mm, with over 70% falling during the rainy season (September to December). The range in average monthly temperature is from 23 to 31°C. Farms were selected in regions where the Australian Centre for International Agricultural Research-funded Project SMCN/2007/109, entitled “Sustainable and Profitable Crop and Livestock Systems for South-Central Coastal Vietnam,” was taking place. Project presence simplified access to commune farms (including permission requirements with local and regional authorities) and permitted adequate experiment tracking due to partnerships with rural development professionals and extension educators operating in the commune. This experiment responded directly to problems identified by local farmers and revealed during baseline project surveys [1, 22].


### 2.2. Farm Selection and Experiment Preparation

Recent field histories for the six farms participating in the experiment included (1)Pennisetum purpureum Schumach. cultivation, (2) vacant with household waste and ash accumulation, (3) cassava and eggplant production, (4) cassava production, (5) peanut production, and (6) vacant lot. A range of backyard sandy soils in Vietnam and potentially in other tropical regions were represented in initial soil fertility conditions (Table 1). Preexperiment (2010) soil samples at a depth of 15 to 25 cm indicated low organic matter, low nutrient concentrations, and acidic soils (Table 1). Soil textural classes consisted of sands and loamy sands with very low cation exchange capacity (Table 1).Table 1
Preexperiment soil fertility levels at 15 to 25 cm depth on six farms in Cát Trinh Commune.
Parameterc
Overall mean
Overall SD
Farm means (n=16)
(n=96)
Farm 1
Farm 2
Farm 3
Farm 4
Farm 5
Farm 6
CEC  (cmol+/kg)a
1.3
0.60
2.4
1.4
1.3
0.9
0.9
1.0
pH (1 : 1 soil : water)
5.67
0.658
5.98
5.53
6.23
5.76
4.63
5.88
OM (g/kg)b
3.5
2.1
5.2
4.5
2.1
1.6
5.1
2.5
S (mg/kg)
9.1
4.47
10.1
9.9
6.3
5.6
17.0
5.6
P (mg/kg)
37
27.3
67
41
68
22
14
12
Ca (mg/kg)
176
68.3
269
204
190
126
116
151
Mg (mg/kg)
29
23.3
71
19
28
17
21
16
K (mg/kg)
36
36.2
91
41
32
11
31
12
Na (mg/kg)
23
13.1
43
20
19
19
21
17
B (mg/kg)
0.18
0.19
0.25
0.38
0.24
0.01
0.15
0.04
Fe (mg/kg)
116
75.2
180
142
153
28
169
27
Mn (mg/kg)
7
7.5
14
7
16
1
2
5
Cu (mg/kg)
1.6
1.29
3.3
2.3
1.6
0.6
1.1
0.6
Zn (mg/kg)
2.7
5.43
4.4
7.1
1.5
0.4
1.0
1.6
Al (mg/kg)d
280
179.7
375
346
235
116
540
72
NO3-N (mg/kg)
3
2.6
5
5
1
1
5
2
NH4-N (mg/kg)
8
4.1
5
15
7
6
8
7
Ca : Mg
7.8
3.79
4.2
12.5
7.2
7.7
5.6
9.4
aCEC: cation exchange capacity determined by summation of cations Ca, Mg, K, Na, and H, according to Ross [15].
bSoil organic matter (OM) was determined by loss-on-ignition at 360°C [16].
cElements are Mehlich-3 extractable nutrients [17].
dAluminum toxicity might be an issue due to low pH (<5) on farm 5.Experimental areas were prepared using animal traction and handheld hoes in July 2010 according to farmer practice. Weeding was done using handheld hoes as needed throughout the experiment (typically one to two times per month). A woven wire fence (1.2 m high) was installed around each area to inhibit animal entry. No pesticides were applied during the experiment.Starter fertilizer (20-20-15; N-P2O5-K2O) was applied at 175 kg/ha before transplantingBrachiaria cv. Mulato II (B. ruziziensis × B. decumbens × B. brizantha) seedlings into experimental plots in September 2010 and a second time after transplanting in October 2010. Mulato II plant establishment density in each 2 × 2 m plot was 20 plants, derived from 50 cm row spacing and 40 cm plant spacing. Replanting was completed during the first two months after establishment (September and October 2010) to obtain desired density (50,000 plants/ha). One-meter buffer strips and outer borders separated plots and contained single row of Mulato II with 40 cm spacing between plants.


### 2.3. Composted Cattle Manure Treatment Preparation, Sampling, and Analyses

Preexperiment compost samples contained 50% DM and 1 g/kg N, which were used to define treatment application rates. Farmer practice in the region dictated compost preparation. Experimental compost treatments were supplied entirely by farm 3 to ensure consistent composition. Compost preparation included daily removal of cattle manure and rice straw refusals from cattle pens into an uncovered pile. Compost was turned at least twice prior to use during the approximately 45 d process. Experimental treatment composition was determined by collecting three representative subsamples (150 g each) of the composted cattle manure during each treatment application period (Table2). The subsamples were dried to stable weight at 60°C in a forced-air oven and ground to pass a 2 mm screen in a Retsch cutting mill (Germany). Brookside Laboratories Inc. (New Bremen, OH) analyzed samples as described in Peters et al. [18]. Total N was determined using an Elementar Vario Max (Elementar Analysensysteme, Hanau, Germany), and minerals were determined using a CEM Mars Express microwave (CEM Corporation, Matthews, NC) with digest analyzed in a Thermo Scientific iCAP 6500 inductively coupled plasma-atomic emission spectrometer (Thermo Electron Corp., Waltman, MA). Ammonium-N and NO3-N were determined using a Lachat QuickChem 8000 flow injection calorimetric analyzer (Lachat Instruments, Loveland, CO). Organic matter (OM) was estimated by loss-on-ignition at 550°C for 2 h [18] and mineral matter was calculated as 100 – %OM. Average compost treatment composition was 50% DM, 261 g OM/kg DM, 12 g N/kg DM, 2733 mg P/kg DM, and 6750 mg K/kg DM (Table 2).Table 2
Means and standard deviations for composition of composted cattle manure from the supplying farm in Cát Trinh Commune on a dry matter basis.
Parameter
Meanc
SDc
Dry matter (%)
50
8.4
Organic matter (g/kg)a
261
99
Mineral matter (g/kg)a
739
99
C : Nb
12.8
2.3
Total N (g/kg)
12
3
Organic N (g/kg)
11
3
P (mg/kg)
2733
1302
K (mg/kg)
6750
4105
Ca (mg/kg)
9988
3581
Mg (mg/kg)
3088
1094
Na (mg/kg)
955
661
S (mg/kg)
2090
516
B (mg/kg)
14
4
Fe (mg/kg)
4266
2196
Mn (mg/kg)
791
129
Cu (mg/kg)
27
25
Zn (mg/kg)
110
25
aOrganic matter was estimated by loss-on-ignition at 550°C for 2 h [18] and mineral matter was calculated as 100 − %OM.
b1.724 factor was applied to convert organic matter (OM) to C, based on the assumption that loss-by-ignition = OM and OM contains 58% organic carbon (Factor of Wolff, as cited in Pribyl  [19]).
cMeans and standard deviations (SD) were calculated from 14 treatment applications, and each treatment application was an average of 3 subsamples.


### 2.4. Experimental Design

Fifteen treatments were derived from the factorial combination of five compost rates (0, 4, 8, 12, and 24 Mg DM/ha per yr) and three urea rates (0, 60, and 120 kg N/ha per yr). Treatments were implemented in a randomized complete block design on six farms (blocks). A sixteenth treatment added 80 kg K2O/ha per yr to the highest compost rate in 2011 and also included the highest urea rate from January 2012 onward. Buffer strips received 4 Mg DM/ha per yr compost.Preexperiment compost samples determined field application rates to achieve desired compost N rates (0, 40, 80, 120, and 240 kg N/ha per yr). Actual N rates (Table3) differed due to variation in composted manure DM and N concentrations over time. Treatments were selected to balance for Mulato II N requirement (240 kg N/ha per yr) with the highest compost treatment. Annual treatments were divided into six parts and applied at approximately 2 mo intervals immediately following each harvest. During the first year, treatments were surface-applied, while second-year treatments were incorporated in the top 15 cm of soil using handheld hoes to reduce potential for nutrient transfer across plots and to reduce nutrient loss. Initial compost and urea treatments were applied in November 2010 (two months after transplanting) after all Mulato II plants were cut uniformly at 25 cm above ground level to initiate the experiment.Table 3
Actual nitrogen (N) treatment rates were combinations of urea N and N in composted cattle manure.
Compost ratea (Mg DM/ha per yr)
Urea ratea
(kg N/ha per yr)
0
60
120
0
0
60
120
4 ± 0.7
46 ± 12.4
106 ± 12.4
166 ± 12.4
8 ± 1.3
92 ± 24.9
152 ± 24.9
212 ± 24.9
12 ± 2.0
138 ± 37.3
198 ± 37.3
258 ± 37.3
24 ± 4.0
276 ± 74.7
336 ± 74.7
396 ± 74.7
aError is standard deviation and exists due to variation in compost composition over time during the experiment.


### 2.5. Sampling Protocols

#### 2.5.1. Soil Samples

Preexperiment soil samples (n=96) were collected in August 2010 using a handheld trowel at a depth of 15 to 25 cm below ground level (Table 1) and postexperiment samples in May 2013 at two depths (0 to 15 cm and 15 to 25 cm). In each plot, three samples were gathered on a diagonal line across the plot and combined into a single composite sample for each depth. Samples were air-dried, litter was removed, and soil was passed through a 2 mm sieve. Brookside Laboratories Inc. (New Bremen, OH) determined soil pH (1 : 1 H2O) [23], using an AS-3000 Dual pH Analyzer, soil OM by loss-on-ignition at 360°C [16], Mehlich-3 [17] extractable P, K, Ca, Mg, S, Na, B, Mn, Cu, Zn, and Al in a Thermo Scientific iCAP 6500 inductively coupled plasma-atomic emission spectrometer (Thermo Electron Corp., Waltman, MA), inorganic N (NH4-N and NO3-N via 1 N KCl cadmium reduction) using a Flow Injection Analyzer (FIAlab Instruments Inc., Bellevue, WA) [24], and cation exchange capacity by summation of cations Ca, Mg, K, Na, and H [15]. Values below the limit of detection were replaced bylimit of detection/2 to enable statistical analysis.


#### 2.5.2. Forage Sampling

Forage plots were harvested every 36 d with SD ± 8.6 d (24 total harvest events) during the experiment, starting in January 2011. Farms were harvested in the same order and within two to four days for each harvest event. All fresh forage biomass was weighed for each plot and small subsamples (20 to 50 g) were collected to determine dry matter concentration. Every second harvest, larger subsamples (200 to 300 g) were collected instead of small subsamples to evaluate forage nutritive value. Samples were dried to stable weight at 60°C in a forced-air oven to determine dry matter concentration. Samples were ground to pass a 4 mm screen in a Retsch cutting mill (Haan, Germany), and those that had not incurred damage from heat or water during the subsequent transportation and storage process were reground to pass a 1 mm screen in a UDY Cyclone Mill (UDY Corp., Fort Collins, CO). September 2011 and December 2012 samples were analyzed by Dairyland Laboratories Inc. (Arcadia, WI) to determine nutritive value using near infrared spectroscopy [25] with a Foss model 5000 (Foss-NIR System, Silver Spring, MD). Measured parameters included N, P, K, Mg, and Ca. Nutrient uptake in September 2011 and December 2012 was used to calculate partial nutrient balances.


#### 2.5.3. Partial Nutrient Balance and Nitrogen Recovery Calculations

Partial nutrient balances for N, P, and K were calculated asinputs-outputs, whereinputs included nutrients applied in fertilizer and compost treatments andoutputs were nutrients accumulated in harvested forage. Nutrient outputs were calculated by multiplying back-transformed mean treatment yields (geometric means) by nutrient concentration for each plot in September 2011 samples and December 2012 samples. Nutrient outputs for farm partial nutrient balances were determined using plot yields for September 2011 and December 2012 (in lieu of overall treatment yields) to capture farm-to-farm yield variation. Nitrogen recovery was calculated according to the difference method of Jokela and Randall [20] as % N recovery = [(N uptake in treatmenti − N uptake in zero N control)/total N applied in treatmenti] × 100. Mean treatment yields for the experiment and September 2011 and December 2012 plot nutrient concentration were used to calculate N uptake.


### 2.6. Composted Manure Incubation Test

An incubation study was conducted with a representative composted manure sample to test if compost addition resulted in a release of nitrate to the soil or a reduction in nitrate due to immobilization. A New York State fine-loamy soil with a relatively high soil nitrate content (pH = 7.6, OM = 64 g/kg, and Morgan NO3-N = 17 mg/kg) (100 g) was amended with 1 g or 10 g representative compost (4 replicates) in 450 mL plastic incubation cups with perforated lids. Cups were arranged in a completely randomized design and incubated at 23°C in the dark. Soil moisture was maintained between 70% and 75% of field capacity during the incubation. After incubation, samples were dried to stable weight at 50°C in a forced-air oven. Subsamples were analyzed for KCl extractable nitrate [26] in an EasyChem Analyzer (Systea Scientific, Oak Brook, IL).


### 2.7. Statistical Methods

The effect of compost and urea treatments on soil fertility factors was evaluated in PROC MIXED [27], with fixed effects of compost, urea, compost × urea, and preexperiment soil fertility, and block as a random effect. Interactions between preexperiment soil fertility factors and main effects were also evaluated to determine if soil fertility responses depended on initial soil fertility levels. Mean differences among treatments were declared at P ≤ 0.05 using Tukey’s statistic to control the familywise error rate for multiple comparisons. One-tailed t-tests were used to assess paired mean differences between preexperiment and postexperiment soil fertility data, with Bonferroni correction used to identify significant mean differences.Partial nutrient balance means for the compost × urea interaction were extracted from mixed models with fixed effects of compost, urea, compost × urea, and harvest period (September 2011 or December 2012), and included block as a random effect. Models estimating farm partial nutrient balances contained fixed effects of compost, urea, and harvest period (September 2011 or December 2012) and block (farm). Nitrogen recovery means for the compost × urea interaction for each harvest period were extracted from mixed models with fixed effects of compost, urea, and compost × urea, and included block as a random effect.


## 2.1. Cát Trinh Commune Characteristics

The experiment was undertaken from 2010 to 2013 in Cát Trinh Commune, Phù Cát District, Bình Định Province in south-central coastal Vietnam. The district capital of Ngô Mây (14°0′2′′N, 109°2′38′′E) borders Cát Trinh Commune to the southwest. Sandy soils in the commune belong to the Arenosols Group [21]. Annual rainfall in the region is about 1,200 mm, with over 70% falling during the rainy season (September to December). The range in average monthly temperature is from 23 to 31°C. Farms were selected in regions where the Australian Centre for International Agricultural Research-funded Project SMCN/2007/109, entitled “Sustainable and Profitable Crop and Livestock Systems for South-Central Coastal Vietnam,” was taking place. Project presence simplified access to commune farms (including permission requirements with local and regional authorities) and permitted adequate experiment tracking due to partnerships with rural development professionals and extension educators operating in the commune. This experiment responded directly to problems identified by local farmers and revealed during baseline project surveys [1, 22].


## 2.2. Farm Selection and Experiment Preparation

Recent field histories for the six farms participating in the experiment included (1)Pennisetum purpureum Schumach. cultivation, (2) vacant with household waste and ash accumulation, (3) cassava and eggplant production, (4) cassava production, (5) peanut production, and (6) vacant lot. A range of backyard sandy soils in Vietnam and potentially in other tropical regions were represented in initial soil fertility conditions (Table 1). Preexperiment (2010) soil samples at a depth of 15 to 25 cm indicated low organic matter, low nutrient concentrations, and acidic soils (Table 1). Soil textural classes consisted of sands and loamy sands with very low cation exchange capacity (Table 1).Table 1
Preexperiment soil fertility levels at 15 to 25 cm depth on six farms in Cát Trinh Commune.
Parameterc
Overall mean
Overall SD
Farm means (n=16)
(n=96)
Farm 1
Farm 2
Farm 3
Farm 4
Farm 5
Farm 6
CEC  (cmol+/kg)a
1.3
0.60
2.4
1.4
1.3
0.9
0.9
1.0
pH (1 : 1 soil : water)
5.67
0.658
5.98
5.53
6.23
5.76
4.63
5.88
OM (g/kg)b
3.5
2.1
5.2
4.5
2.1
1.6
5.1
2.5
S (mg/kg)
9.1
4.47
10.1
9.9
6.3
5.6
17.0
5.6
P (mg/kg)
37
27.3
67
41
68
22
14
12
Ca (mg/kg)
176
68.3
269
204
190
126
116
151
Mg (mg/kg)
29
23.3
71
19
28
17
21
16
K (mg/kg)
36
36.2
91
41
32
11
31
12
Na (mg/kg)
23
13.1
43
20
19
19
21
17
B (mg/kg)
0.18
0.19
0.25
0.38
0.24
0.01
0.15
0.04
Fe (mg/kg)
116
75.2
180
142
153
28
169
27
Mn (mg/kg)
7
7.5
14
7
16
1
2
5
Cu (mg/kg)
1.6
1.29
3.3
2.3
1.6
0.6
1.1
0.6
Zn (mg/kg)
2.7
5.43
4.4
7.1
1.5
0.4
1.0
1.6
Al (mg/kg)d
280
179.7
375
346
235
116
540
72
NO3-N (mg/kg)
3
2.6
5
5
1
1
5
2
NH4-N (mg/kg)
8
4.1
5
15
7
6
8
7
Ca : Mg
7.8
3.79
4.2
12.5
7.2
7.7
5.6
9.4
aCEC: cation exchange capacity determined by summation of cations Ca, Mg, K, Na, and H, according to Ross [15].
bSoil organic matter (OM) was determined by loss-on-ignition at 360°C [16].
cElements are Mehlich-3 extractable nutrients [17].
dAluminum toxicity might be an issue due to low pH (<5) on farm 5.Experimental areas were prepared using animal traction and handheld hoes in July 2010 according to farmer practice. Weeding was done using handheld hoes as needed throughout the experiment (typically one to two times per month). A woven wire fence (1.2 m high) was installed around each area to inhibit animal entry. No pesticides were applied during the experiment.Starter fertilizer (20-20-15; N-P2O5-K2O) was applied at 175 kg/ha before transplantingBrachiaria cv. Mulato II (B. ruziziensis × B. decumbens × B. brizantha) seedlings into experimental plots in September 2010 and a second time after transplanting in October 2010. Mulato II plant establishment density in each 2 × 2 m plot was 20 plants, derived from 50 cm row spacing and 40 cm plant spacing. Replanting was completed during the first two months after establishment (September and October 2010) to obtain desired density (50,000 plants/ha). One-meter buffer strips and outer borders separated plots and contained single row of Mulato II with 40 cm spacing between plants.


## 2.3. Composted Cattle Manure Treatment Preparation, Sampling, and Analyses

Preexperiment compost samples contained 50% DM and 1 g/kg N, which were used to define treatment application rates. Farmer practice in the region dictated compost preparation. Experimental compost treatments were supplied entirely by farm 3 to ensure consistent composition. Compost preparation included daily removal of cattle manure and rice straw refusals from cattle pens into an uncovered pile. Compost was turned at least twice prior to use during the approximately 45 d process. Experimental treatment composition was determined by collecting three representative subsamples (150 g each) of the composted cattle manure during each treatment application period (Table2). The subsamples were dried to stable weight at 60°C in a forced-air oven and ground to pass a 2 mm screen in a Retsch cutting mill (Germany). Brookside Laboratories Inc. (New Bremen, OH) analyzed samples as described in Peters et al. [18]. Total N was determined using an Elementar Vario Max (Elementar Analysensysteme, Hanau, Germany), and minerals were determined using a CEM Mars Express microwave (CEM Corporation, Matthews, NC) with digest analyzed in a Thermo Scientific iCAP 6500 inductively coupled plasma-atomic emission spectrometer (Thermo Electron Corp., Waltman, MA). Ammonium-N and NO3-N were determined using a Lachat QuickChem 8000 flow injection calorimetric analyzer (Lachat Instruments, Loveland, CO). Organic matter (OM) was estimated by loss-on-ignition at 550°C for 2 h [18] and mineral matter was calculated as 100 – %OM. Average compost treatment composition was 50% DM, 261 g OM/kg DM, 12 g N/kg DM, 2733 mg P/kg DM, and 6750 mg K/kg DM (Table 2).Table 2
Means and standard deviations for composition of composted cattle manure from the supplying farm in Cát Trinh Commune on a dry matter basis.
Parameter
Meanc
SDc
Dry matter (%)
50
8.4
Organic matter (g/kg)a
261
99
Mineral matter (g/kg)a
739
99
C : Nb
12.8
2.3
Total N (g/kg)
12
3
Organic N (g/kg)
11
3
P (mg/kg)
2733
1302
K (mg/kg)
6750
4105
Ca (mg/kg)
9988
3581
Mg (mg/kg)
3088
1094
Na (mg/kg)
955
661
S (mg/kg)
2090
516
B (mg/kg)
14
4
Fe (mg/kg)
4266
2196
Mn (mg/kg)
791
129
Cu (mg/kg)
27
25
Zn (mg/kg)
110
25
aOrganic matter was estimated by loss-on-ignition at 550°C for 2 h [18] and mineral matter was calculated as 100 − %OM.
b1.724 factor was applied to convert organic matter (OM) to C, based on the assumption that loss-by-ignition = OM and OM contains 58% organic carbon (Factor of Wolff, as cited in Pribyl  [19]).
cMeans and standard deviations (SD) were calculated from 14 treatment applications, and each treatment application was an average of 3 subsamples.


## 2.4. Experimental Design

Fifteen treatments were derived from the factorial combination of five compost rates (0, 4, 8, 12, and 24 Mg DM/ha per yr) and three urea rates (0, 60, and 120 kg N/ha per yr). Treatments were implemented in a randomized complete block design on six farms (blocks). A sixteenth treatment added 80 kg K2O/ha per yr to the highest compost rate in 2011 and also included the highest urea rate from January 2012 onward. Buffer strips received 4 Mg DM/ha per yr compost.Preexperiment compost samples determined field application rates to achieve desired compost N rates (0, 40, 80, 120, and 240 kg N/ha per yr). Actual N rates (Table3) differed due to variation in composted manure DM and N concentrations over time. Treatments were selected to balance for Mulato II N requirement (240 kg N/ha per yr) with the highest compost treatment. Annual treatments were divided into six parts and applied at approximately 2 mo intervals immediately following each harvest. During the first year, treatments were surface-applied, while second-year treatments were incorporated in the top 15 cm of soil using handheld hoes to reduce potential for nutrient transfer across plots and to reduce nutrient loss. Initial compost and urea treatments were applied in November 2010 (two months after transplanting) after all Mulato II plants were cut uniformly at 25 cm above ground level to initiate the experiment.Table 3
Actual nitrogen (N) treatment rates were combinations of urea N and N in composted cattle manure.
Compost ratea (Mg DM/ha per yr)
Urea ratea
(kg N/ha per yr)
0
60
120
0
0
60
120
4 ± 0.7
46 ± 12.4
106 ± 12.4
166 ± 12.4
8 ± 1.3
92 ± 24.9
152 ± 24.9
212 ± 24.9
12 ± 2.0
138 ± 37.3
198 ± 37.3
258 ± 37.3
24 ± 4.0
276 ± 74.7
336 ± 74.7
396 ± 74.7
aError is standard deviation and exists due to variation in compost composition over time during the experiment.


## 2.5. Sampling Protocols

### 2.5.1. Soil Samples

Preexperiment soil samples (n=96) were collected in August 2010 using a handheld trowel at a depth of 15 to 25 cm below ground level (Table 1) and postexperiment samples in May 2013 at two depths (0 to 15 cm and 15 to 25 cm). In each plot, three samples were gathered on a diagonal line across the plot and combined into a single composite sample for each depth. Samples were air-dried, litter was removed, and soil was passed through a 2 mm sieve. Brookside Laboratories Inc. (New Bremen, OH) determined soil pH (1 : 1 H2O) [23], using an AS-3000 Dual pH Analyzer, soil OM by loss-on-ignition at 360°C [16], Mehlich-3 [17] extractable P, K, Ca, Mg, S, Na, B, Mn, Cu, Zn, and Al in a Thermo Scientific iCAP 6500 inductively coupled plasma-atomic emission spectrometer (Thermo Electron Corp., Waltman, MA), inorganic N (NH4-N and NO3-N via 1 N KCl cadmium reduction) using a Flow Injection Analyzer (FIAlab Instruments Inc., Bellevue, WA) [24], and cation exchange capacity by summation of cations Ca, Mg, K, Na, and H [15]. Values below the limit of detection were replaced bylimit of detection/2 to enable statistical analysis.


### 2.5.2. Forage Sampling

Forage plots were harvested every 36 d with SD ± 8.6 d (24 total harvest events) during the experiment, starting in January 2011. Farms were harvested in the same order and within two to four days for each harvest event. All fresh forage biomass was weighed for each plot and small subsamples (20 to 50 g) were collected to determine dry matter concentration. Every second harvest, larger subsamples (200 to 300 g) were collected instead of small subsamples to evaluate forage nutritive value. Samples were dried to stable weight at 60°C in a forced-air oven to determine dry matter concentration. Samples were ground to pass a 4 mm screen in a Retsch cutting mill (Haan, Germany), and those that had not incurred damage from heat or water during the subsequent transportation and storage process were reground to pass a 1 mm screen in a UDY Cyclone Mill (UDY Corp., Fort Collins, CO). September 2011 and December 2012 samples were analyzed by Dairyland Laboratories Inc. (Arcadia, WI) to determine nutritive value using near infrared spectroscopy [25] with a Foss model 5000 (Foss-NIR System, Silver Spring, MD). Measured parameters included N, P, K, Mg, and Ca. Nutrient uptake in September 2011 and December 2012 was used to calculate partial nutrient balances.


### 2.5.3. Partial Nutrient Balance and Nitrogen Recovery Calculations

Partial nutrient balances for N, P, and K were calculated asinputs-outputs, whereinputs included nutrients applied in fertilizer and compost treatments andoutputs were nutrients accumulated in harvested forage. Nutrient outputs were calculated by multiplying back-transformed mean treatment yields (geometric means) by nutrient concentration for each plot in September 2011 samples and December 2012 samples. Nutrient outputs for farm partial nutrient balances were determined using plot yields for September 2011 and December 2012 (in lieu of overall treatment yields) to capture farm-to-farm yield variation. Nitrogen recovery was calculated according to the difference method of Jokela and Randall [20] as % N recovery = [(N uptake in treatmenti − N uptake in zero N control)/total N applied in treatmenti] × 100. Mean treatment yields for the experiment and September 2011 and December 2012 plot nutrient concentration were used to calculate N uptake.


## 2.5.1. Soil Samples

Preexperiment soil samples (n=96) were collected in August 2010 using a handheld trowel at a depth of 15 to 25 cm below ground level (Table 1) and postexperiment samples in May 2013 at two depths (0 to 15 cm and 15 to 25 cm). In each plot, three samples were gathered on a diagonal line across the plot and combined into a single composite sample for each depth. Samples were air-dried, litter was removed, and soil was passed through a 2 mm sieve. Brookside Laboratories Inc. (New Bremen, OH) determined soil pH (1 : 1 H2O) [23], using an AS-3000 Dual pH Analyzer, soil OM by loss-on-ignition at 360°C [16], Mehlich-3 [17] extractable P, K, Ca, Mg, S, Na, B, Mn, Cu, Zn, and Al in a Thermo Scientific iCAP 6500 inductively coupled plasma-atomic emission spectrometer (Thermo Electron Corp., Waltman, MA), inorganic N (NH4-N and NO3-N via 1 N KCl cadmium reduction) using a Flow Injection Analyzer (FIAlab Instruments Inc., Bellevue, WA) [24], and cation exchange capacity by summation of cations Ca, Mg, K, Na, and H [15]. Values below the limit of detection were replaced bylimit of detection/2 to enable statistical analysis.


## 2.5.2. Forage Sampling

Forage plots were harvested every 36 d with SD ± 8.6 d (24 total harvest events) during the experiment, starting in January 2011. Farms were harvested in the same order and within two to four days for each harvest event. All fresh forage biomass was weighed for each plot and small subsamples (20 to 50 g) were collected to determine dry matter concentration. Every second harvest, larger subsamples (200 to 300 g) were collected instead of small subsamples to evaluate forage nutritive value. Samples were dried to stable weight at 60°C in a forced-air oven to determine dry matter concentration. Samples were ground to pass a 4 mm screen in a Retsch cutting mill (Haan, Germany), and those that had not incurred damage from heat or water during the subsequent transportation and storage process were reground to pass a 1 mm screen in a UDY Cyclone Mill (UDY Corp., Fort Collins, CO). September 2011 and December 2012 samples were analyzed by Dairyland Laboratories Inc. (Arcadia, WI) to determine nutritive value using near infrared spectroscopy [25] with a Foss model 5000 (Foss-NIR System, Silver Spring, MD). Measured parameters included N, P, K, Mg, and Ca. Nutrient uptake in September 2011 and December 2012 was used to calculate partial nutrient balances.


## 2.5.3. Partial Nutrient Balance and Nitrogen Recovery Calculations

Partial nutrient balances for N, P, and K were calculated asinputs-outputs, whereinputs included nutrients applied in fertilizer and compost treatments andoutputs were nutrients accumulated in harvested forage. Nutrient outputs were calculated by multiplying back-transformed mean treatment yields (geometric means) by nutrient concentration for each plot in September 2011 samples and December 2012 samples. Nutrient outputs for farm partial nutrient balances were determined using plot yields for September 2011 and December 2012 (in lieu of overall treatment yields) to capture farm-to-farm yield variation. Nitrogen recovery was calculated according to the difference method of Jokela and Randall [20] as % N recovery = [(N uptake in treatmenti − N uptake in zero N control)/total N applied in treatmenti] × 100. Mean treatment yields for the experiment and September 2011 and December 2012 plot nutrient concentration were used to calculate N uptake.


## 2.6. Composted Manure Incubation Test

An incubation study was conducted with a representative composted manure sample to test if compost addition resulted in a release of nitrate to the soil or a reduction in nitrate due to immobilization. A New York State fine-loamy soil with a relatively high soil nitrate content (pH = 7.6, OM = 64 g/kg, and Morgan NO3-N = 17 mg/kg) (100 g) was amended with 1 g or 10 g representative compost (4 replicates) in 450 mL plastic incubation cups with perforated lids. Cups were arranged in a completely randomized design and incubated at 23°C in the dark. Soil moisture was maintained between 70% and 75% of field capacity during the incubation. After incubation, samples were dried to stable weight at 50°C in a forced-air oven. Subsamples were analyzed for KCl extractable nitrate [26] in an EasyChem Analyzer (Systea Scientific, Oak Brook, IL).


## 2.7. Statistical Methods

The effect of compost and urea treatments on soil fertility factors was evaluated in PROC MIXED [27], with fixed effects of compost, urea, compost × urea, and preexperiment soil fertility, and block as a random effect. Interactions between preexperiment soil fertility factors and main effects were also evaluated to determine if soil fertility responses depended on initial soil fertility levels. Mean differences among treatments were declared at P ≤ 0.05 using Tukey’s statistic to control the familywise error rate for multiple comparisons. One-tailed t-tests were used to assess paired mean differences between preexperiment and postexperiment soil fertility data, with Bonferroni correction used to identify significant mean differences.Partial nutrient balance means for the compost × urea interaction were extracted from mixed models with fixed effects of compost, urea, compost × urea, and harvest period (September 2011 or December 2012), and included block as a random effect. Models estimating farm partial nutrient balances contained fixed effects of compost, urea, and harvest period (September 2011 or December 2012) and block (farm). Nitrogen recovery means for the compost × urea interaction for each harvest period were extracted from mixed models with fixed effects of compost, urea, and compost × urea, and included block as a random effect.


## 3. Results

### 3.1. Soil Fertility

#### 3.1.1. Preexperiment and Postexperiment Soil Fertility

Preexperiment farm soil fertility levels were consistent with yield differences observed in the experiment (i.e., preexperiment soil nutrient concentrations were higher for high-yielding farms). All treatment means (n=6 for each compost × urea interaction rate) were numerically higher in postexperiment samples in the 15 to 25 cm stratum than preexperiment samples at the same depth for OM and NH4-N, and most means were numerically higher after the experiment for Na and Fe (Table 8). More than half of postexperiment treatment means were also numerically higher than preexperiment for Cu and Mn. All treatment means were numerically lower in postexperiment samples at the 15 to 25 cm stratum than preexperiment samples at the same depth for S, P, K, and NO3-N, and most were lower postexperiment for pH, Mg, Al, Ca, and CEC. Significant mean difference (Bonferroni correction) was detected only in soil Na for compost applied at 12 Mg DM/ha per yr without urea, which was higher after the experiment (Table 8).Models estimating soil fertility parameters in the 15 to 25 cm stratum (postexperiment level –preexperiment level) indicated that compost impacted differences for soil Al (P=0.0189, ordered 12, 24, 4, 8, and 0, with 12 > 8 and 0) (Table 9). Urea application decreased soil OM (P=0.0036, ordered 0, 60, and 120, with 0 and 60 > 120) and tended to affect Mg (P=0.0898, ordered 0, 60, and 120, no significant differences) (Table 4). A compost × urea interaction was detected for K (P=0.0413, no significant differences), with a tendency to impact pH (P=0.0595), Zn (P=0.0816), and NH4+ (P=0.0985).Table 4
Soil chemical models in 0 to 15 cm and 15 to 25 cm strata selected from candidate fixed effects of compost (C), urea (U), compost × urea (C × U), preexperiment soil fertility for each parameter sampled at 15 to 25 cm depth (PSF), and significant interactions, with block included as a random effect.
Parameterb
0 to 15 cm depth
15 to 25 cm depth
Model effect
Farm (%)
Model effect
Farm (%)c
S (mg/kg)
C × U × PSFa
82
C × U × PSFa
85
Mg (mg/kg)
C × U × PSFa
87
C × U × PSFa
89
Zn (mg/kg)
C × U × PSFa
28
C × U × PSFa
16
Mn (mg/kg)
C × PSFa
87
C × U × PSFa
49
OM (g/kg)
C × U × PSFa
65
U × PSFa
77
K (mg/kg)
C × PSFa
51
C × PSFa
60
Cu (mg/kg)
C × U × PSFa
62
PSF
53
CEC (cmol+/kg)
PSF
31
C × U × PSFa
46
P (mg/kg)
PSF
52
C × U × PSFa
28
NH4-N (mg/kg)
PSF
66
PSF C × Ua
42
pH (1 : 1 soil : water)
C PSF
88
PSF
75
Fe (mg/kg)
PSF
88
PSF
70
Al (mg/kg)
PSF
62
PSF
58
NO3-N (mg/kg)
PSF
43
PSF
46
Ca (mg/kg)
C × Ua
75
PSF
49
Na (mg/kg)
PSF
47
C × Ua
49
B (mg/kg)
NS
—
NS
—
aWhere interactions were significant, only highest level interactive effect or effects are shown, although lower level terms were also in the model.
bEach row represents a single model for each soil depth.
cPercentage of residual variance explained by farm in each model is indicated.Preexperiment soil factors (PSF) most highly associated with yield were Mn (r=0.48), P (r=0.44), Fe (r=0.33), exchange capacity (r=0.32), and Ca (r=0.32). Many preexperiment soil fertility factors were significant in multivariate models with compost and urea main effects (Table 10). Models selected from main effects, PSFs, and significant interactions indicated the importance of preexperiment soil fertility on treatment responses for some soil fertility parameters (Table 4). The effect of compost and urea (main effects) on postexperiment soil S, K, Mg, Zn, Mn, and OM depended on their respective PSFs for both soil depths. Compost and urea effects on soil Cu depended on Cu PSF in the 0 to 15 cm stratum, while only Cu PSF emerged from the 15 to 25 cm stratum. The effect of compost and urea on soil CEC and P depended on PSF in the 15 to 25 cm stratum, while only PSF mattered in the 0 to 15 cm stratum. The effect of compost and urea on soil pH at 0 to 15 cm and NH4-N at 15 to 25 cm did not depend on PSF, although it was in the model. Only PSF emerged from models estimating Fe, Al, and NO3-N at both depths, CEC, P, NH4-N, and Na at 0 to 15 cm, and Ca, pH, and Cu at 15 to 25 cm. Preexperiment soil fertility was not significant for Ca at 0 to 15 cm or for Na at 15 to 25 cm. Farm explained greater than 40% of residual variation in soil fertility models at both depths for all factors except P, Zn, and CEC.Compost addition increased soil pH in both soil strata (Figure1, Table 10), although pH for the 12 Mg DM/ha per yr compost rate was lower than the 24 Mg DM/ha per yr rate. Postexperiment soil pH was highest where compost had been applied, although pH decreased numerically in most plots relative to preexperiment levels (Table 8). Preexperiment soil pH (1 : 1 soil : water) was higher than postexperiment pH for all treatments in the 15 to 25 cm stratum, which suggests minor soil acidification during the experiment. The difference between post- and preexperiment pH was less for the highest compost treatment than 12 Mg DM/ha per yr. Postexperiment pH was impacted by compost treatments. Soil pH in the 0 to 15 cm stratum for the highest compost rate (pH = 5.58) was higher than without compost (pH = 5.37) and 12 Mg DM/ha per yr (pH = 5.33) (Figure 1). Response to compost in the 15 to 25 cm stratum indicated that pH for the highest compost rate (pH = 5.53) was higher than for the 12 Mg DM/ha per yr (pH = 5.24) treatment. Overall, the deeper layer was slightly more acidic than the topsoil layer (Figure 1).Figure 1
Postexperiment pH least squares means for composted manure rates in the 0 to 15 cm ((a),P=0.0071) and 15 to 25 cm strata ((b), P=0.0460) and soil organic matter (OM) least square means for compost treatments in the 0 to 15 cm stratum ((c), P=0.0087) and urea treatments in the 15 to 25 cm stratum ((d), P=0.0035). pH models contained preexperiment soil pH and OM models contained preexperiment OM (Table 10). Error bars are ±1 SE of the mean. Bars connected by the same letter are not significantly different (P ≤ 0.05).
(a)
0 to 15 cm
(b)
15 to 25 cm
(c)
0 to 15 cm
(d)
15 to 25 cmCompost increased soil OM in the 0 to 15 cm stratum (Figure1, Table 10). Urea application decreased OM in the 15 to 25 cm stratum (Figure 1, Table 10). Organic matter for the highest urea rate was lower than the intermediate rate and plots not receiving urea, possibly explained by high mineralization rates in this stratum combined with limited OM addition to the soil in the form of plant litter. Organic matter in the 15 to 25 cm stratum for treatment plots increased by 0.8 g/kg to 3.8 g/kg from preexperiment to postexperiment levels (Table 8).Consistent with the OM effect, postexperiment Ca in the 0 to 15 cm stratum increased steadily with added compost (Figure2) and likely contributed to buffering capacity (Figure 2, Table 10). Calcium was higher for the highest compost rate than all other compost treatments. Compost × urea interaction was also observed (results not shown), but the interaction response was inconsistent. Calcium also increased numerically with applied compost in the 15 to 25 cm stratum.Figure 2
Postexperiment soil Ca least squares means for composted manure rates in the 0 to 15 cm ((a),P<0.0001) and 15 to 25 cm strata ((b), P=0.1873), Mg least squares means in the 0 to 15 cm ((c), P=0.0015) and 15 to 25 cm ((d), P=0.0542) strata, and Mn least squares means for compost treatments in the 0 to 15 cm stratum ((e), P=0.0002) and for urea treatments in the 15 to 25 cm stratum ((f), P=0.2015). Error bars are ±1 SE of the mean. Bars connected by the same letter are not significantly different (P ≤ 0.05). Models contain preexperiment soil fertility factors (Table 10).
(a)
0 to 15 cm
(b)
15 to 25 cm
(c)
0 to 15 cm
(d)
15 to 25 cm
(e)
0 to 15 cm
(f)
15 to 25 cmSoil Mg increased with compost treatments in the 0 to 15 cm stratum, and a trend was observed in the 15 to 25 cm stratum (Figure2, Table 10). Similar to soil pH, Mg for the highest compost rate was numerically higher than all other treatments and soil Mg tended to increase with compost application (excepting the 12 Mg DM/ha per yr plots).Soil Mn in the 0 to 15 cm stratum increased with compost addition, and Mn for the highest compost rate was higher than 4 Mg DM/ha per yr and plots not receiving compost (Figure2, Table 10). Similar to OM, Mn at 15 to 25 cm depth decreased with added urea, although the effect was not significant (P=0.2015).Soil Na responses in the 15 to 25 cm stratum were probably due to an outlying treatment response. The compost × urea interaction effects for soil Na and soil NH4-N in this stratum were inconsistent (results not shown). Preexperiment soil Al levels tended to be higher than postexperiment levels in the 15 to 25 cm stratum (Table 8). Soil Al for compost applied at 12 Mg DM/ha per yr was higher (lower mean difference) than 8 Mg DM/ha per yr and without compost (Table 9). This suggests soil Al depletion during the experiment, especially for plots receiving no or a low rate of compost. Compost effect on Al was not detected in statistical analyses of postexperiment data.Observed soil fertility effects could be a function of pre- or postharvest sampling time. Preexperiment samples were collected in August 2010 and postexperiment samples in early May 2013. These soil sampling time frames were both during the dry season but rainfall was slightly higher leading up to August 2010 sampling than May 2013. Soil moisture near sampling times (not measured) could have affected mineralization rates and outcomes.


#### 3.1.2. Incubation Study Results

The compost incubation study confirmed that addition of composted cattle manure at a high rate resulted in rapid N mineralization and nitrification as expected for material with C : N of 13 : 1 (Table5). The incubation study did not provide evidence of N immobilization, consistent with the relatively low C : N ratio of the compost. However, at the lower application rate, no increase in soil nitrate was determined. The latter supports the hypothesis that the lack of a yield response to compost addition in the farm experiments is due to an insufficient amount applied and not due to immobilization.Table 5
Nitrate-N extracted from compost-amended soil, control, and nonincubated soil in a laboratory incubation study.
Treatment
N treatment equivalent
Soil nitrate-Na
SE
(g dry compost added to 100 g dry soil)
(kg N/ha)
(mg/kg)
10
2,735
161.5 a
3.36
1
273.5
15.7 b
3.36
0
0
19.5 b
3.36
Nonincubated soil
—
15.2 b
3.36
aTreatments connected by the same letter are not significantly different (P≤0.05).


### 3.2. Partial Nutrient Balances and Nitrogen Recovery

Nutrient concentration across the two sampling dates averaged 24 g N/kg DM, 3.8 g P/kg DM, 21.2 g K/kg DM, 3.5 g Mg/kg DM, and 6.9 g Ca/kg DM. Average partial N, P, and K balances were negative on five of the six farms for N and P and negative on all six farms for K (Table6). Overall partial N and P balances on lower-yielding farms were positive or less negative (farms 4, 5, and 6) than for higher-yielding farms (Table 6). Farm-to-farm variation explained a large proportion of residual variance in partial nutrient balances (39% in N balance model, 27% in P balance model, and 52% in K balance model).Table 6
Brachiaria cv. Mulato II dry matter yield and N, P, and K balances by farm.
Farm
Yielda
N balancebc
P balancebc
K balancebc
(Mg DM/ha/yr)
(kg N/ha per yr)
(kg P/ha per yr)
(kg K/ha per yr)
3
26.2
−653 e
−113 d
−783 e
1
16.1
−342 d
−71 c
−610 d
2
14.8
−325 d
−57 c
−388 c
5
13.3
−150 c
−21 b
−186 b
6
8.8
−56 b
−9 ab
−107 ab
4
6.6
53 a
7 a
−38 a
aFarm yields are overall experiment means (geometric means).
bFarm N, P, and K balances were calculated using dry matter yield and plot nutrient concentrations for the September 2011 and December 2012 samples.
cMeans not connected by the same letter in each column are significantly different (P ≤ 0.05).Farms with better soil fertility indicators yielded higher, but this also resulted in more negative partial N balances (Table6). Variable farm plot histories were also responsible for differences in farm yields. Partial N balance (Table 7) was most negative for treatments containing low N inputs and progressively became less negative with additional N applications as urea and compost. Only the highest compost rate achieved partial N balances that were close to zero or positive (Table 7). Partial P and K balances by farm followed a similar pattern to N, but all P and K balances were negative (Table 7). Treatments receiving higher rates of compost were less negative or positive in P balance. Potassium balances were very negative, impacted more by yield than by compost rates, reflecting the fact that compost K inputs (0, 26.6, 53.2, 79.9, and 159.7 kg K/ha per yr) were low relative to K removal with harvested forage. Dry season partial nutrient balances were higher due to lower DM yields (not shown).Table 7
Dry matter yield, partial N, P, and K balances, and nitrogen recovery, by composted manure and urea treatment combinations.
Compost rate
Urea rate
Yield
N balanceab
P balanceab
K balanceab
Nitrogen recoverybc
Sept 2011
Dec 2012
(Mg DM/ha per yr)
(kg N/ha per yr)
(Mg DM/ha per yr)
(kg N/ha per yr)
(kg P/ha per yr)
(kg K/ha per yr)
(%)
(%)
24
120
18.3
−67 bc
−5 b
−241 bc
34.0 ab
42.2 ab
24
60
12.5
32 a
17 a
−123 a
3.0 b
−7.8 b
24
0
11.7
−8 ab
20 a
−104 a
−8.5 b
−12.4 b
12
120
15.4
−113 cd
−25 d
−258 cd
22.4 ab
23.0 b
12
60
15.7
−168 def
−25 d
−247 bc
21.4 ab
32.7 ab
12
0
12.2
−153 de
−13 c
−192 b
−10.7 b
−20.4
8
120
15.3
−155 de
−36 e
−263 cd
21.0 ab
30.3 ab
8
60
13.3
−160 de
−28 d
−230 bc
0.5 b
−0.6 b
8
0
12.8
−208 efg
−26 d
−216 bc
−13.1 b
−13.0 b
4
120
14.1
−181 def
−42 fg
−248 bc
23.4 ab
17.9 b
4
60
16.7
−307 hi
−52 h
−316 d
78.5 a
112.2 a
4
0
12.8
−264 ghi
−38 ef
−240 bc
1.1 b
−10.8 b
0
120
13.6
−202 efg
−49 h
−280 cd
10.6 ab
5.0 b
0
60
12.5
−236 fgh
−47 gh
−276 cd
−13.5 b
−42.2 b
0
0
12.6
−312 i
−47 gh
−240 bc
—
—
aBalances were calculated using overall dry matter yield treatment means and plot treatment nutrient concentrations from September 2011 and December 2012 samples.
bMeans not connected by the same letter in each column are significantly different (P≤0.05).
cNitrogen recovery was calculated according to Jokela and Randall [20] as  % N recovery = [(N uptake in treatmenti − N uptake in zero N control)/total N applied in treatmenti] × 100. Nitrogen recovery was based on overall dry matter yield treatment means and application rates in September 2011 and December 2012.Table 8
Soil fertility means before and after the experiment at the 15 to 25 cm depth for compost × urea treatment combinations. Elements are Mehlich-3 extractable nutrients. Data from before the experiment are included as a treatment baseline. Means calculated in each cell are an average of 6 farms. The only pair that differed significantly based on a paired one-tailedt-test, using the Bonferroni-adjusted P value cutoff of 0.003, is in bold.
Parameter
Compost treatment (Mg DM/ha per yr) / urea treatment (kg N/ha per yr)
0
4
8
12
24
0
4
8
12
24
0
4
8
12
24
0
0
0
0
0
60
60
60
60
60
120
120
120
120
120
Before pH (1 : 1 soil : water)
5.37
5.98
5.63
5.87
5.68
5.52
5.27
5.83
5.83
5.63
5.72
5.50
5.82
5.75
5.45
After  pH (1 : 1 soil : water)
5.05
5.30
5.47
5.48
5.67
5.58
5.05
5.48
5.37
5.50
5.18
5.32
5.32
5.10
5.32
Before CEC  (cmol+/kg)
1.19
1.16
1.19
1.22
1.53
1.52
1.20
1.40
1.29
1.43
1.33
1.17
1.35
1.28
1.23
After  CEC  (cmol+/kg)
0.922
1.10
1.13
1.16
1.47
1.69
1.05
1.26
1.26
1.26
1.14
1.04
1.31
1.44
1.25
Before OM (g/kg)
4.07
2.58
2.87
3.05
4.15
4.68
3.28
3.35
3.13
3.87
3.98
3.47
3.15
4.22
3.15
After  OM (g/kg)
6.82
5.5
5.97
6.65
7.23
6.62
7.12
6.13
6.1
7.48
4.8
4.98
5.53
6.32
5.58
Before S (mg/kg)
9.67
8.00
9.33
7.50
9.50
9.67
9.33
8.67
8.67
10.30
9.83
9.17
8.17
8.50
9.00
After  S (mg/kg)
8.67
6.83
7.17
7.33
7.67
6.67
8.50
7.00
7.67
7.50
8.33
7.33
7.67
7.67
7.33
Before P (mg/kg)
36.5
37.7
36.5
27.2
39.0
50.8
33.2
46.2
34.2
39.7
36.2
37.3
42.8
29.3
37.3
After  P (mg/kg)
25.3
18.5
24.2
23.5
36.5
29.0
27.7
26.5
22.0
24.2
23.2
20.7
25.3
17.0
24.2
Before Ca (mg/kg)
165
159
165
178
207
198
170
193
159
201
174
162
179
165
150
After  Ca (mg/kg)
119
156
154
163
220
181
141
168
185
174
136
141
187
176
169
Before Mg (mg/kg)
25.8
22.0
23.5
22.0
30.2
31.8
22.8
31.3
33.2
29.2
34.3
23.5
30.7
27.7
34.2
After  Mg (mg/kg)
20.8
20.2
23.8
24.8
26.7
24.0
21.7
23.5
21.5
26.5
20.0
19.7
25.8
16.2
30.5
Before K (mg/kg)
24.3
35.5
32.7
23.2
47.3
64.8
25.7
29.5
48.3
32.5
26.5
29.5
38.7
33.5
38.2
After  K (mg/kg)
12.3
10.7
10.8
11.8
10.2
16.0
12.2
12.0
11.0
12.5
11.0
11.5
11.8
10.7
12.0
Before Na (mg/kg)
21.3
20.7
20.5
18.8
28.8
21.2
20.3
22.5
21.3
23.7
24.2
20.8
24.5
32.5
22.7
After  Na (mg/kg)
28.2
29.7
29.3
25.0
27.5
42.3
30.5
28.2
28.0
32.0
26.3
32.0
28.8
28.3
27.8
Before B (mg/kg)
0.135
0.170
0.228
0.208
0.243
0.203
0.135
0.148
0.100
0.262
0.230
0.075
0.203
0.127
0.138
After  B (mg/kg)
0.262
0.115
0.125
0.090
0.170
0.290
0.223
0.100
0.207
0.128
0.300
0.235
0.143
0.183
0.313
Before Fe (mg/kg)
132
123
116
97.8
116
153
106
127
115
132
100
128
107
109
114
After  Fe (mg/kg)
138
112
120
132
148
154
125
135
127
125
108
122
152
120
121
Before Mn (mg/kg)
8.50
5.83
5.17
8.17
8.17
10.5
6.17
5.67
8.00
6.00
6.33
6.67
8.83
11.5
5.33
After  Mn (mg/kg)
6.50
7.00
10.0
8.00
13.7
8.00
9.67
7.00
11.0
6.17
6.17
5.33
9.33
7.50
6.17
Before Cu (mg/kg)
1.74
1.11
1.19
1.24
1.76
1.45
1.20
1.50
1.69
1.50
2.05
1.94
1.17
2.39
1.56
After  Cu (mg/kg)
1.81
1.65
2.02
1.67
1.73
1.73
1.74
1.59
1.44
2.05
1.99
1.69
2.24
2.02
1.68
Before Zn (mg/kg)
2.45
1.45
2.06
3.20
3.14
2.72
1.88
1.64
1.76
2.11
3.69
2.09
2.05
10.60
1.61
After  Zn (mg/kg)
1.37
2.12
2.17
5.97
3.53
4.13
1.84
1.59
1.89
2.42
3.57
1.98
3.89
2.25
1.60
Before Al (mg/kg)
293
235
306
244
273
285
289
339
246
267
369
292
249
225
265
After  Al (mg/kg)
250
192
245
257
254
221
267
248
230
202
313
210
245
230
242
Before NO3-N (mg/kg)
3.93
2.40
2.40
2.67
5.10
3.87
3.15
1.90
3.32
2.68
1.67
2.78
3.48
4.57
2.45
After  NO3-N (mg/kg)
1.37
1.33
0.77
1.25
0.400
0.450
1.87
0.533
1.48
0.650
0.850
0.433
1.98
2.57
1.12
Before NH4-N (mg/kg)
8.90
6.13
8.30
6.73
7.60
8.88
8.57
8.65
8.72
8.17
9.73
8.25
7.72
6.70
7.77
After  NH4-N (mg/kg)
13.5
9.52
9.82
10.2
10.1
10.2
12.4
9.92
10.6
10.7
11.0
10.4
13.5
10.8
10.6Table 9
TreatmentPvalues for the difference between postexperiment soil fertility parameters and preexperiment parameters in the 15 to 25 cm stratum. Each row represents a single model. Models include block as a random effect. Elemental analyses are for Mehlich-3 extractable elements. Significant effects (P ≤ 0.05) are in bold.
Parameter
Compost
Urea
Compost × urea
Total exchange capacity (cmol+/kg)
0.9317
0.8727
0.8756
pH (1 : 1 soil : water)
0.0269
0.1749
0.0595
Organic matter (g/kg)
0.1604
0.0036
0.7973
S (mg/kg)
0.3240
0.4647
0.7185
P (mg/kg)
0.4661
0.2771
0.4179
Ca (mg/kg)
0.4523
0.8386
0.8740
Mg (mg/kg)
0.3694
0.0898
0.6977
K (mg/kg)
0.7658
0.4829
0.0413
Na (mg/kg)
0.2887
0.1182
0.4206
B (mg/kg)
0.2849
0.1422
0.5266
Fe (mg/kg)
0.3824
0.6983
0.1219
Mn (mg/kg)
0.2557
0.2068
0.4542
Cu (mg/kg)
0.1078
0.4458
0.1693
Zn (mg/kg)
0.7921
0.2485
0.0816
Al (mg/kg)
0.0189
0.2622
0.1371
NO3-N (mg/kg)
0.4310
0.4914
0.1622
NH4-N (mg/kg)
0.9296
0.3301
0.0985Table 10
P values for treatment effects on soil chemical properties in 0 to 15 cm and 15 to 25 cm strata for models containing fixed effects of compost (C), urea (U), compost × urea (C × U), and preexperiment soil fertility measurement for each parameter sampled at 15 to 25 cm depth (PSF), with block as a random effect. Each row represents a single model for each soil depth. Significant values at P ≤ 0.05 are in bold.
Parameter
C
U
C × U
PSF
C
U
C × U
PSF
0 to 15 cm depth
15 to 25 cm depth
CEC  (cmol+/kg)
0.2145
0.6271
0.3142
0.0797
0.6827
0.5997
0.4332
0.0363
pH (1 : 1 soil : water)
0.0071
0.1196
0.0705
<0.0001
0.0460
0.1071
0.0856
<0.0001
OM (g/kg)
0.0087
0.6440
0.0505
<0.0001
0.2699
0.0035
0.7938
<0.0001
S (mg/kg)
0.2249
0.9163
0.0720
0.2507
0.8146
0.7345
0.1171
0.0547
P (mg/kg)
0.6723
0.5556
0.3621
0.0157
0.6537
0.3633
0.4554
0.0001
Ca (mg/kg)
<0.0001
0.4688
0.0147
0.0718
0.1873
0.9323
0.6124
0.2071
Mg (mg/kg)
0.0015
0.2469
0.2219
0.0540
0.0542
0.4886
0.4991
0.0006
K (mg/kg)
0.1703
0.3593
0.7202
0.0301
0.2833
0.1041
0.3959
0.3572
Na (mg/kg)
0.0788
0.6333
0.8865
0.0446
0.1636
0.0246
0.0201
0.3319
B (mg/kg)
0.0954
0.4529
0.4883
0.9517
0.0788
0.2146
0.8916
0.5975
Fe (mg/kg)
0.9956
0.4733
0.2342
0.0020
0.4978
0.9109
0.1584
<0.0001
Mn (mg/kg)
0.0002
0.4951
0.6742
0.9859
0.5170
0.2015
0.3686
0.0002
Cu (mg/kg)
0.5569
0.3744
0.5829
0.0343
0.3963
0.8292
0.4546
0.0003
Zn (mg/kg)
0.8383
0.6481
0.2006
0.1849
0.9202
0.5418
0.0771
0.0046
Al (mg/kg)
0.6086
0.4611
0.6980
<0.0001
0.1240
0.2865
0.1456
<0.0001
NO3-N (mg/kg)
0.5608
0.2398
0.2026
0.1510
0.1936
0.4103
0.2641
0.0885
NH4-N (mg/kg)
0.1017
0.6409
0.1306
0.0155
0.9530
0.5630
0.0386
0.0068Greatest N recovery occurred in plots receiving the highest rate of urea (ranging from 5% to 42%) (Table7). Highest overall N recovery was observed with 60 kg N/ha per yr urea × 4 Mg DM/ha per yr compost (79% and 112%, depending on nutritive value period) due to high dry matter yield for this treatment. Farm-to-farm variation explained 38% of residual variation in N recovery for September 2011 samples and 65% for December 2012.


## 3.1. Soil Fertility

### 3.1.1. Preexperiment and Postexperiment Soil Fertility

Preexperiment farm soil fertility levels were consistent with yield differences observed in the experiment (i.e., preexperiment soil nutrient concentrations were higher for high-yielding farms). All treatment means (n=6 for each compost × urea interaction rate) were numerically higher in postexperiment samples in the 15 to 25 cm stratum than preexperiment samples at the same depth for OM and NH4-N, and most means were numerically higher after the experiment for Na and Fe (Table 8). More than half of postexperiment treatment means were also numerically higher than preexperiment for Cu and Mn. All treatment means were numerically lower in postexperiment samples at the 15 to 25 cm stratum than preexperiment samples at the same depth for S, P, K, and NO3-N, and most were lower postexperiment for pH, Mg, Al, Ca, and CEC. Significant mean difference (Bonferroni correction) was detected only in soil Na for compost applied at 12 Mg DM/ha per yr without urea, which was higher after the experiment (Table 8).Models estimating soil fertility parameters in the 15 to 25 cm stratum (postexperiment level –preexperiment level) indicated that compost impacted differences for soil Al (P=0.0189, ordered 12, 24, 4, 8, and 0, with 12 > 8 and 0) (Table 9). Urea application decreased soil OM (P=0.0036, ordered 0, 60, and 120, with 0 and 60 > 120) and tended to affect Mg (P=0.0898, ordered 0, 60, and 120, no significant differences) (Table 4). A compost × urea interaction was detected for K (P=0.0413, no significant differences), with a tendency to impact pH (P=0.0595), Zn (P=0.0816), and NH4+ (P=0.0985).Table 4
Soil chemical models in 0 to 15 cm and 15 to 25 cm strata selected from candidate fixed effects of compost (C), urea (U), compost × urea (C × U), preexperiment soil fertility for each parameter sampled at 15 to 25 cm depth (PSF), and significant interactions, with block included as a random effect.
Parameterb
0 to 15 cm depth
15 to 25 cm depth
Model effect
Farm (%)
Model effect
Farm (%)c
S (mg/kg)
C × U × PSFa
82
C × U × PSFa
85
Mg (mg/kg)
C × U × PSFa
87
C × U × PSFa
89
Zn (mg/kg)
C × U × PSFa
28
C × U × PSFa
16
Mn (mg/kg)
C × PSFa
87
C × U × PSFa
49
OM (g/kg)
C × U × PSFa
65
U × PSFa
77
K (mg/kg)
C × PSFa
51
C × PSFa
60
Cu (mg/kg)
C × U × PSFa
62
PSF
53
CEC (cmol+/kg)
PSF
31
C × U × PSFa
46
P (mg/kg)
PSF
52
C × U × PSFa
28
NH4-N (mg/kg)
PSF
66
PSF C × Ua
42
pH (1 : 1 soil : water)
C PSF
88
PSF
75
Fe (mg/kg)
PSF
88
PSF
70
Al (mg/kg)
PSF
62
PSF
58
NO3-N (mg/kg)
PSF
43
PSF
46
Ca (mg/kg)
C × Ua
75
PSF
49
Na (mg/kg)
PSF
47
C × Ua
49
B (mg/kg)
NS
—
NS
—
aWhere interactions were significant, only highest level interactive effect or effects are shown, although lower level terms were also in the model.
bEach row represents a single model for each soil depth.
cPercentage of residual variance explained by farm in each model is indicated.Preexperiment soil factors (PSF) most highly associated with yield were Mn (r=0.48), P (r=0.44), Fe (r=0.33), exchange capacity (r=0.32), and Ca (r=0.32). Many preexperiment soil fertility factors were significant in multivariate models with compost and urea main effects (Table 10). Models selected from main effects, PSFs, and significant interactions indicated the importance of preexperiment soil fertility on treatment responses for some soil fertility parameters (Table 4). The effect of compost and urea (main effects) on postexperiment soil S, K, Mg, Zn, Mn, and OM depended on their respective PSFs for both soil depths. Compost and urea effects on soil Cu depended on Cu PSF in the 0 to 15 cm stratum, while only Cu PSF emerged from the 15 to 25 cm stratum. The effect of compost and urea on soil CEC and P depended on PSF in the 15 to 25 cm stratum, while only PSF mattered in the 0 to 15 cm stratum. The effect of compost and urea on soil pH at 0 to 15 cm and NH4-N at 15 to 25 cm did not depend on PSF, although it was in the model. Only PSF emerged from models estimating Fe, Al, and NO3-N at both depths, CEC, P, NH4-N, and Na at 0 to 15 cm, and Ca, pH, and Cu at 15 to 25 cm. Preexperiment soil fertility was not significant for Ca at 0 to 15 cm or for Na at 15 to 25 cm. Farm explained greater than 40% of residual variation in soil fertility models at both depths for all factors except P, Zn, and CEC.Compost addition increased soil pH in both soil strata (Figure1, Table 10), although pH for the 12 Mg DM/ha per yr compost rate was lower than the 24 Mg DM/ha per yr rate. Postexperiment soil pH was highest where compost had been applied, although pH decreased numerically in most plots relative to preexperiment levels (Table 8). Preexperiment soil pH (1 : 1 soil : water) was higher than postexperiment pH for all treatments in the 15 to 25 cm stratum, which suggests minor soil acidification during the experiment. The difference between post- and preexperiment pH was less for the highest compost treatment than 12 Mg DM/ha per yr. Postexperiment pH was impacted by compost treatments. Soil pH in the 0 to 15 cm stratum for the highest compost rate (pH = 5.58) was higher than without compost (pH = 5.37) and 12 Mg DM/ha per yr (pH = 5.33) (Figure 1). Response to compost in the 15 to 25 cm stratum indicated that pH for the highest compost rate (pH = 5.53) was higher than for the 12 Mg DM/ha per yr (pH = 5.24) treatment. Overall, the deeper layer was slightly more acidic than the topsoil layer (Figure 1).Figure 1
Postexperiment pH least squares means for composted manure rates in the 0 to 15 cm ((a),P=0.0071) and 15 to 25 cm strata ((b), P=0.0460) and soil organic matter (OM) least square means for compost treatments in the 0 to 15 cm stratum ((c), P=0.0087) and urea treatments in the 15 to 25 cm stratum ((d), P=0.0035). pH models contained preexperiment soil pH and OM models contained preexperiment OM (Table 10). Error bars are ±1 SE of the mean. Bars connected by the same letter are not significantly different (P ≤ 0.05).
(a)
0 to 15 cm
(b)
15 to 25 cm
(c)
0 to 15 cm
(d)
15 to 25 cmCompost increased soil OM in the 0 to 15 cm stratum (Figure1, Table 10). Urea application decreased OM in the 15 to 25 cm stratum (Figure 1, Table 10). Organic matter for the highest urea rate was lower than the intermediate rate and plots not receiving urea, possibly explained by high mineralization rates in this stratum combined with limited OM addition to the soil in the form of plant litter. Organic matter in the 15 to 25 cm stratum for treatment plots increased by 0.8 g/kg to 3.8 g/kg from preexperiment to postexperiment levels (Table 8).Consistent with the OM effect, postexperiment Ca in the 0 to 15 cm stratum increased steadily with added compost (Figure2) and likely contributed to buffering capacity (Figure 2, Table 10). Calcium was higher for the highest compost rate than all other compost treatments. Compost × urea interaction was also observed (results not shown), but the interaction response was inconsistent. Calcium also increased numerically with applied compost in the 15 to 25 cm stratum.Figure 2
Postexperiment soil Ca least squares means for composted manure rates in the 0 to 15 cm ((a),P<0.0001) and 15 to 25 cm strata ((b), P=0.1873), Mg least squares means in the 0 to 15 cm ((c), P=0.0015) and 15 to 25 cm ((d), P=0.0542) strata, and Mn least squares means for compost treatments in the 0 to 15 cm stratum ((e), P=0.0002) and for urea treatments in the 15 to 25 cm stratum ((f), P=0.2015). Error bars are ±1 SE of the mean. Bars connected by the same letter are not significantly different (P ≤ 0.05). Models contain preexperiment soil fertility factors (Table 10).
(a)
0 to 15 cm
(b)
15 to 25 cm
(c)
0 to 15 cm
(d)
15 to 25 cm
(e)
0 to 15 cm
(f)
15 to 25 cmSoil Mg increased with compost treatments in the 0 to 15 cm stratum, and a trend was observed in the 15 to 25 cm stratum (Figure2, Table 10). Similar to soil pH, Mg for the highest compost rate was numerically higher than all other treatments and soil Mg tended to increase with compost application (excepting the 12 Mg DM/ha per yr plots).Soil Mn in the 0 to 15 cm stratum increased with compost addition, and Mn for the highest compost rate was higher than 4 Mg DM/ha per yr and plots not receiving compost (Figure2, Table 10). Similar to OM, Mn at 15 to 25 cm depth decreased with added urea, although the effect was not significant (P=0.2015).Soil Na responses in the 15 to 25 cm stratum were probably due to an outlying treatment response. The compost × urea interaction effects for soil Na and soil NH4-N in this stratum were inconsistent (results not shown). Preexperiment soil Al levels tended to be higher than postexperiment levels in the 15 to 25 cm stratum (Table 8). Soil Al for compost applied at 12 Mg DM/ha per yr was higher (lower mean difference) than 8 Mg DM/ha per yr and without compost (Table 9). This suggests soil Al depletion during the experiment, especially for plots receiving no or a low rate of compost. Compost effect on Al was not detected in statistical analyses of postexperiment data.Observed soil fertility effects could be a function of pre- or postharvest sampling time. Preexperiment samples were collected in August 2010 and postexperiment samples in early May 2013. These soil sampling time frames were both during the dry season but rainfall was slightly higher leading up to August 2010 sampling than May 2013. Soil moisture near sampling times (not measured) could have affected mineralization rates and outcomes.


### 3.1.2. Incubation Study Results

The compost incubation study confirmed that addition of composted cattle manure at a high rate resulted in rapid N mineralization and nitrification as expected for material with C : N of 13 : 1 (Table5). The incubation study did not provide evidence of N immobilization, consistent with the relatively low C : N ratio of the compost. However, at the lower application rate, no increase in soil nitrate was determined. The latter supports the hypothesis that the lack of a yield response to compost addition in the farm experiments is due to an insufficient amount applied and not due to immobilization.Table 5
Nitrate-N extracted from compost-amended soil, control, and nonincubated soil in a laboratory incubation study.
Treatment
N treatment equivalent
Soil nitrate-Na
SE
(g dry compost added to 100 g dry soil)
(kg N/ha)
(mg/kg)
10
2,735
161.5 a
3.36
1
273.5
15.7 b
3.36
0
0
19.5 b
3.36
Nonincubated soil
—
15.2 b
3.36
aTreatments connected by the same letter are not significantly different (P≤0.05).


## 3.1.1. Preexperiment and Postexperiment Soil Fertility

Preexperiment farm soil fertility levels were consistent with yield differences observed in the experiment (i.e., preexperiment soil nutrient concentrations were higher for high-yielding farms). All treatment means (n=6 for each compost × urea interaction rate) were numerically higher in postexperiment samples in the 15 to 25 cm stratum than preexperiment samples at the same depth for OM and NH4-N, and most means were numerically higher after the experiment for Na and Fe (Table 8). More than half of postexperiment treatment means were also numerically higher than preexperiment for Cu and Mn. All treatment means were numerically lower in postexperiment samples at the 15 to 25 cm stratum than preexperiment samples at the same depth for S, P, K, and NO3-N, and most were lower postexperiment for pH, Mg, Al, Ca, and CEC. Significant mean difference (Bonferroni correction) was detected only in soil Na for compost applied at 12 Mg DM/ha per yr without urea, which was higher after the experiment (Table 8).Models estimating soil fertility parameters in the 15 to 25 cm stratum (postexperiment level –preexperiment level) indicated that compost impacted differences for soil Al (P=0.0189, ordered 12, 24, 4, 8, and 0, with 12 > 8 and 0) (Table 9). Urea application decreased soil OM (P=0.0036, ordered 0, 60, and 120, with 0 and 60 > 120) and tended to affect Mg (P=0.0898, ordered 0, 60, and 120, no significant differences) (Table 4). A compost × urea interaction was detected for K (P=0.0413, no significant differences), with a tendency to impact pH (P=0.0595), Zn (P=0.0816), and NH4+ (P=0.0985).Table 4
Soil chemical models in 0 to 15 cm and 15 to 25 cm strata selected from candidate fixed effects of compost (C), urea (U), compost × urea (C × U), preexperiment soil fertility for each parameter sampled at 15 to 25 cm depth (PSF), and significant interactions, with block included as a random effect.
Parameterb
0 to 15 cm depth
15 to 25 cm depth
Model effect
Farm (%)
Model effect
Farm (%)c
S (mg/kg)
C × U × PSFa
82
C × U × PSFa
85
Mg (mg/kg)
C × U × PSFa
87
C × U × PSFa
89
Zn (mg/kg)
C × U × PSFa
28
C × U × PSFa
16
Mn (mg/kg)
C × PSFa
87
C × U × PSFa
49
OM (g/kg)
C × U × PSFa
65
U × PSFa
77
K (mg/kg)
C × PSFa
51
C × PSFa
60
Cu (mg/kg)
C × U × PSFa
62
PSF
53
CEC (cmol+/kg)
PSF
31
C × U × PSFa
46
P (mg/kg)
PSF
52
C × U × PSFa
28
NH4-N (mg/kg)
PSF
66
PSF C × Ua
42
pH (1 : 1 soil : water)
C PSF
88
PSF
75
Fe (mg/kg)
PSF
88
PSF
70
Al (mg/kg)
PSF
62
PSF
58
NO3-N (mg/kg)
PSF
43
PSF
46
Ca (mg/kg)
C × Ua
75
PSF
49
Na (mg/kg)
PSF
47
C × Ua
49
B (mg/kg)
NS
—
NS
—
aWhere interactions were significant, only highest level interactive effect or effects are shown, although lower level terms were also in the model.
bEach row represents a single model for each soil depth.
cPercentage of residual variance explained by farm in each model is indicated.Preexperiment soil factors (PSF) most highly associated with yield were Mn (r=0.48), P (r=0.44), Fe (r=0.33), exchange capacity (r=0.32), and Ca (r=0.32). Many preexperiment soil fertility factors were significant in multivariate models with compost and urea main effects (Table 10). Models selected from main effects, PSFs, and significant interactions indicated the importance of preexperiment soil fertility on treatment responses for some soil fertility parameters (Table 4). The effect of compost and urea (main effects) on postexperiment soil S, K, Mg, Zn, Mn, and OM depended on their respective PSFs for both soil depths. Compost and urea effects on soil Cu depended on Cu PSF in the 0 to 15 cm stratum, while only Cu PSF emerged from the 15 to 25 cm stratum. The effect of compost and urea on soil CEC and P depended on PSF in the 15 to 25 cm stratum, while only PSF mattered in the 0 to 15 cm stratum. The effect of compost and urea on soil pH at 0 to 15 cm and NH4-N at 15 to 25 cm did not depend on PSF, although it was in the model. Only PSF emerged from models estimating Fe, Al, and NO3-N at both depths, CEC, P, NH4-N, and Na at 0 to 15 cm, and Ca, pH, and Cu at 15 to 25 cm. Preexperiment soil fertility was not significant for Ca at 0 to 15 cm or for Na at 15 to 25 cm. Farm explained greater than 40% of residual variation in soil fertility models at both depths for all factors except P, Zn, and CEC.Compost addition increased soil pH in both soil strata (Figure1, Table 10), although pH for the 12 Mg DM/ha per yr compost rate was lower than the 24 Mg DM/ha per yr rate. Postexperiment soil pH was highest where compost had been applied, although pH decreased numerically in most plots relative to preexperiment levels (Table 8). Preexperiment soil pH (1 : 1 soil : water) was higher than postexperiment pH for all treatments in the 15 to 25 cm stratum, which suggests minor soil acidification during the experiment. The difference between post- and preexperiment pH was less for the highest compost treatment than 12 Mg DM/ha per yr. Postexperiment pH was impacted by compost treatments. Soil pH in the 0 to 15 cm stratum for the highest compost rate (pH = 5.58) was higher than without compost (pH = 5.37) and 12 Mg DM/ha per yr (pH = 5.33) (Figure 1). Response to compost in the 15 to 25 cm stratum indicated that pH for the highest compost rate (pH = 5.53) was higher than for the 12 Mg DM/ha per yr (pH = 5.24) treatment. Overall, the deeper layer was slightly more acidic than the topsoil layer (Figure 1).Figure 1
Postexperiment pH least squares means for composted manure rates in the 0 to 15 cm ((a),P=0.0071) and 15 to 25 cm strata ((b), P=0.0460) and soil organic matter (OM) least square means for compost treatments in the 0 to 15 cm stratum ((c), P=0.0087) and urea treatments in the 15 to 25 cm stratum ((d), P=0.0035). pH models contained preexperiment soil pH and OM models contained preexperiment OM (Table 10). Error bars are ±1 SE of the mean. Bars connected by the same letter are not significantly different (P ≤ 0.05).
(a)
0 to 15 cm
(b)
15 to 25 cm
(c)
0 to 15 cm
(d)
15 to 25 cmCompost increased soil OM in the 0 to 15 cm stratum (Figure1, Table 10). Urea application decreased OM in the 15 to 25 cm stratum (Figure 1, Table 10). Organic matter for the highest urea rate was lower than the intermediate rate and plots not receiving urea, possibly explained by high mineralization rates in this stratum combined with limited OM addition to the soil in the form of plant litter. Organic matter in the 15 to 25 cm stratum for treatment plots increased by 0.8 g/kg to 3.8 g/kg from preexperiment to postexperiment levels (Table 8).Consistent with the OM effect, postexperiment Ca in the 0 to 15 cm stratum increased steadily with added compost (Figure2) and likely contributed to buffering capacity (Figure 2, Table 10). Calcium was higher for the highest compost rate than all other compost treatments. Compost × urea interaction was also observed (results not shown), but the interaction response was inconsistent. Calcium also increased numerically with applied compost in the 15 to 25 cm stratum.Figure 2
Postexperiment soil Ca least squares means for composted manure rates in the 0 to 15 cm ((a),P<0.0001) and 15 to 25 cm strata ((b), P=0.1873), Mg least squares means in the 0 to 15 cm ((c), P=0.0015) and 15 to 25 cm ((d), P=0.0542) strata, and Mn least squares means for compost treatments in the 0 to 15 cm stratum ((e), P=0.0002) and for urea treatments in the 15 to 25 cm stratum ((f), P=0.2015). Error bars are ±1 SE of the mean. Bars connected by the same letter are not significantly different (P ≤ 0.05). Models contain preexperiment soil fertility factors (Table 10).
(a)
0 to 15 cm
(b)
15 to 25 cm
(c)
0 to 15 cm
(d)
15 to 25 cm
(e)
0 to 15 cm
(f)
15 to 25 cmSoil Mg increased with compost treatments in the 0 to 15 cm stratum, and a trend was observed in the 15 to 25 cm stratum (Figure2, Table 10). Similar to soil pH, Mg for the highest compost rate was numerically higher than all other treatments and soil Mg tended to increase with compost application (excepting the 12 Mg DM/ha per yr plots).Soil Mn in the 0 to 15 cm stratum increased with compost addition, and Mn for the highest compost rate was higher than 4 Mg DM/ha per yr and plots not receiving compost (Figure2, Table 10). Similar to OM, Mn at 15 to 25 cm depth decreased with added urea, although the effect was not significant (P=0.2015).Soil Na responses in the 15 to 25 cm stratum were probably due to an outlying treatment response. The compost × urea interaction effects for soil Na and soil NH4-N in this stratum were inconsistent (results not shown). Preexperiment soil Al levels tended to be higher than postexperiment levels in the 15 to 25 cm stratum (Table 8). Soil Al for compost applied at 12 Mg DM/ha per yr was higher (lower mean difference) than 8 Mg DM/ha per yr and without compost (Table 9). This suggests soil Al depletion during the experiment, especially for plots receiving no or a low rate of compost. Compost effect on Al was not detected in statistical analyses of postexperiment data.Observed soil fertility effects could be a function of pre- or postharvest sampling time. Preexperiment samples were collected in August 2010 and postexperiment samples in early May 2013. These soil sampling time frames were both during the dry season but rainfall was slightly higher leading up to August 2010 sampling than May 2013. Soil moisture near sampling times (not measured) could have affected mineralization rates and outcomes.


## 3.1.2. Incubation Study Results

The compost incubation study confirmed that addition of composted cattle manure at a high rate resulted in rapid N mineralization and nitrification as expected for material with C : N of 13 : 1 (Table5). The incubation study did not provide evidence of N immobilization, consistent with the relatively low C : N ratio of the compost. However, at the lower application rate, no increase in soil nitrate was determined. The latter supports the hypothesis that the lack of a yield response to compost addition in the farm experiments is due to an insufficient amount applied and not due to immobilization.Table 5
Nitrate-N extracted from compost-amended soil, control, and nonincubated soil in a laboratory incubation study.
Treatment
N treatment equivalent
Soil nitrate-Na
SE
(g dry compost added to 100 g dry soil)
(kg N/ha)
(mg/kg)
10
2,735
161.5 a
3.36
1
273.5
15.7 b
3.36
0
0
19.5 b
3.36
Nonincubated soil
—
15.2 b
3.36
aTreatments connected by the same letter are not significantly different (P≤0.05).


## 3.2. Partial Nutrient Balances and Nitrogen Recovery

Nutrient concentration across the two sampling dates averaged 24 g N/kg DM, 3.8 g P/kg DM, 21.2 g K/kg DM, 3.5 g Mg/kg DM, and 6.9 g Ca/kg DM. Average partial N, P, and K balances were negative on five of the six farms for N and P and negative on all six farms for K (Table6). Overall partial N and P balances on lower-yielding farms were positive or less negative (farms 4, 5, and 6) than for higher-yielding farms (Table 6). Farm-to-farm variation explained a large proportion of residual variance in partial nutrient balances (39% in N balance model, 27% in P balance model, and 52% in K balance model).Table 6
Brachiaria cv. Mulato II dry matter yield and N, P, and K balances by farm.
Farm
Yielda
N balancebc
P balancebc
K balancebc
(Mg DM/ha/yr)
(kg N/ha per yr)
(kg P/ha per yr)
(kg K/ha per yr)
3
26.2
−653 e
−113 d
−783 e
1
16.1
−342 d
−71 c
−610 d
2
14.8
−325 d
−57 c
−388 c
5
13.3
−150 c
−21 b
−186 b
6
8.8
−56 b
−9 ab
−107 ab
4
6.6
53 a
7 a
−38 a
aFarm yields are overall experiment means (geometric means).
bFarm N, P, and K balances were calculated using dry matter yield and plot nutrient concentrations for the September 2011 and December 2012 samples.
cMeans not connected by the same letter in each column are significantly different (P ≤ 0.05).Farms with better soil fertility indicators yielded higher, but this also resulted in more negative partial N balances (Table6). Variable farm plot histories were also responsible for differences in farm yields. Partial N balance (Table 7) was most negative for treatments containing low N inputs and progressively became less negative with additional N applications as urea and compost. Only the highest compost rate achieved partial N balances that were close to zero or positive (Table 7). Partial P and K balances by farm followed a similar pattern to N, but all P and K balances were negative (Table 7). Treatments receiving higher rates of compost were less negative or positive in P balance. Potassium balances were very negative, impacted more by yield than by compost rates, reflecting the fact that compost K inputs (0, 26.6, 53.2, 79.9, and 159.7 kg K/ha per yr) were low relative to K removal with harvested forage. Dry season partial nutrient balances were higher due to lower DM yields (not shown).Table 7
Dry matter yield, partial N, P, and K balances, and nitrogen recovery, by composted manure and urea treatment combinations.
Compost rate
Urea rate
Yield
N balanceab
P balanceab
K balanceab
Nitrogen recoverybc
Sept 2011
Dec 2012
(Mg DM/ha per yr)
(kg N/ha per yr)
(Mg DM/ha per yr)
(kg N/ha per yr)
(kg P/ha per yr)
(kg K/ha per yr)
(%)
(%)
24
120
18.3
−67 bc
−5 b
−241 bc
34.0 ab
42.2 ab
24
60
12.5
32 a
17 a
−123 a
3.0 b
−7.8 b
24
0
11.7
−8 ab
20 a
−104 a
−8.5 b
−12.4 b
12
120
15.4
−113 cd
−25 d
−258 cd
22.4 ab
23.0 b
12
60
15.7
−168 def
−25 d
−247 bc
21.4 ab
32.7 ab
12
0
12.2
−153 de
−13 c
−192 b
−10.7 b
−20.4
8
120
15.3
−155 de
−36 e
−263 cd
21.0 ab
30.3 ab
8
60
13.3
−160 de
−28 d
−230 bc
0.5 b
−0.6 b
8
0
12.8
−208 efg
−26 d
−216 bc
−13.1 b
−13.0 b
4
120
14.1
−181 def
−42 fg
−248 bc
23.4 ab
17.9 b
4
60
16.7
−307 hi
−52 h
−316 d
78.5 a
112.2 a
4
0
12.8
−264 ghi
−38 ef
−240 bc
1.1 b
−10.8 b
0
120
13.6
−202 efg
−49 h
−280 cd
10.6 ab
5.0 b
0
60
12.5
−236 fgh
−47 gh
−276 cd
−13.5 b
−42.2 b
0
0
12.6
−312 i
−47 gh
−240 bc
—
—
aBalances were calculated using overall dry matter yield treatment means and plot treatment nutrient concentrations from September 2011 and December 2012 samples.
bMeans not connected by the same letter in each column are significantly different (P≤0.05).
cNitrogen recovery was calculated according to Jokela and Randall [20] as  % N recovery = [(N uptake in treatmenti − N uptake in zero N control)/total N applied in treatmenti] × 100. Nitrogen recovery was based on overall dry matter yield treatment means and application rates in September 2011 and December 2012.Table 8
Soil fertility means before and after the experiment at the 15 to 25 cm depth for compost × urea treatment combinations. Elements are Mehlich-3 extractable nutrients. Data from before the experiment are included as a treatment baseline. Means calculated in each cell are an average of 6 farms. The only pair that differed significantly based on a paired one-tailedt-test, using the Bonferroni-adjusted P value cutoff of 0.003, is in bold.
Parameter
Compost treatment (Mg DM/ha per yr) / urea treatment (kg N/ha per yr)
0
4
8
12
24
0
4
8
12
24
0
4
8
12
24
0
0
0
0
0
60
60
60
60
60
120
120
120
120
120
Before pH (1 : 1 soil : water)
5.37
5.98
5.63
5.87
5.68
5.52
5.27
5.83
5.83
5.63
5.72
5.50
5.82
5.75
5.45
After  pH (1 : 1 soil : water)
5.05
5.30
5.47
5.48
5.67
5.58
5.05
5.48
5.37
5.50
5.18
5.32
5.32
5.10
5.32
Before CEC  (cmol+/kg)
1.19
1.16
1.19
1.22
1.53
1.52
1.20
1.40
1.29
1.43
1.33
1.17
1.35
1.28
1.23
After  CEC  (cmol+/kg)
0.922
1.10
1.13
1.16
1.47
1.69
1.05
1.26
1.26
1.26
1.14
1.04
1.31
1.44
1.25
Before OM (g/kg)
4.07
2.58
2.87
3.05
4.15
4.68
3.28
3.35
3.13
3.87
3.98
3.47
3.15
4.22
3.15
After  OM (g/kg)
6.82
5.5
5.97
6.65
7.23
6.62
7.12
6.13
6.1
7.48
4.8
4.98
5.53
6.32
5.58
Before S (mg/kg)
9.67
8.00
9.33
7.50
9.50
9.67
9.33
8.67
8.67
10.30
9.83
9.17
8.17
8.50
9.00
After  S (mg/kg)
8.67
6.83
7.17
7.33
7.67
6.67
8.50
7.00
7.67
7.50
8.33
7.33
7.67
7.67
7.33
Before P (mg/kg)
36.5
37.7
36.5
27.2
39.0
50.8
33.2
46.2
34.2
39.7
36.2
37.3
42.8
29.3
37.3
After  P (mg/kg)
25.3
18.5
24.2
23.5
36.5
29.0
27.7
26.5
22.0
24.2
23.2
20.7
25.3
17.0
24.2
Before Ca (mg/kg)
165
159
165
178
207
198
170
193
159
201
174
162
179
165
150
After  Ca (mg/kg)
119
156
154
163
220
181
141
168
185
174
136
141
187
176
169
Before Mg (mg/kg)
25.8
22.0
23.5
22.0
30.2
31.8
22.8
31.3
33.2
29.2
34.3
23.5
30.7
27.7
34.2
After  Mg (mg/kg)
20.8
20.2
23.8
24.8
26.7
24.0
21.7
23.5
21.5
26.5
20.0
19.7
25.8
16.2
30.5
Before K (mg/kg)
24.3
35.5
32.7
23.2
47.3
64.8
25.7
29.5
48.3
32.5
26.5
29.5
38.7
33.5
38.2
After  K (mg/kg)
12.3
10.7
10.8
11.8
10.2
16.0
12.2
12.0
11.0
12.5
11.0
11.5
11.8
10.7
12.0
Before Na (mg/kg)
21.3
20.7
20.5
18.8
28.8
21.2
20.3
22.5
21.3
23.7
24.2
20.8
24.5
32.5
22.7
After  Na (mg/kg)
28.2
29.7
29.3
25.0
27.5
42.3
30.5
28.2
28.0
32.0
26.3
32.0
28.8
28.3
27.8
Before B (mg/kg)
0.135
0.170
0.228
0.208
0.243
0.203
0.135
0.148
0.100
0.262
0.230
0.075
0.203
0.127
0.138
After  B (mg/kg)
0.262
0.115
0.125
0.090
0.170
0.290
0.223
0.100
0.207
0.128
0.300
0.235
0.143
0.183
0.313
Before Fe (mg/kg)
132
123
116
97.8
116
153
106
127
115
132
100
128
107
109
114
After  Fe (mg/kg)
138
112
120
132
148
154
125
135
127
125
108
122
152
120
121
Before Mn (mg/kg)
8.50
5.83
5.17
8.17
8.17
10.5
6.17
5.67
8.00
6.00
6.33
6.67
8.83
11.5
5.33
After  Mn (mg/kg)
6.50
7.00
10.0
8.00
13.7
8.00
9.67
7.00
11.0
6.17
6.17
5.33
9.33
7.50
6.17
Before Cu (mg/kg)
1.74
1.11
1.19
1.24
1.76
1.45
1.20
1.50
1.69
1.50
2.05
1.94
1.17
2.39
1.56
After  Cu (mg/kg)
1.81
1.65
2.02
1.67
1.73
1.73
1.74
1.59
1.44
2.05
1.99
1.69
2.24
2.02
1.68
Before Zn (mg/kg)
2.45
1.45
2.06
3.20
3.14
2.72
1.88
1.64
1.76
2.11
3.69
2.09
2.05
10.60
1.61
After  Zn (mg/kg)
1.37
2.12
2.17
5.97
3.53
4.13
1.84
1.59
1.89
2.42
3.57
1.98
3.89
2.25
1.60
Before Al (mg/kg)
293
235
306
244
273
285
289
339
246
267
369
292
249
225
265
After  Al (mg/kg)
250
192
245
257
254
221
267
248
230
202
313
210
245
230
242
Before NO3-N (mg/kg)
3.93
2.40
2.40
2.67
5.10
3.87
3.15
1.90
3.32
2.68
1.67
2.78
3.48
4.57
2.45
After  NO3-N (mg/kg)
1.37
1.33
0.77
1.25
0.400
0.450
1.87
0.533
1.48
0.650
0.850
0.433
1.98
2.57
1.12
Before NH4-N (mg/kg)
8.90
6.13
8.30
6.73
7.60
8.88
8.57
8.65
8.72
8.17
9.73
8.25
7.72
6.70
7.77
After  NH4-N (mg/kg)
13.5
9.52
9.82
10.2
10.1
10.2
12.4
9.92
10.6
10.7
11.0
10.4
13.5
10.8
10.6Table 9
TreatmentPvalues for the difference between postexperiment soil fertility parameters and preexperiment parameters in the 15 to 25 cm stratum. Each row represents a single model. Models include block as a random effect. Elemental analyses are for Mehlich-3 extractable elements. Significant effects (P ≤ 0.05) are in bold.
Parameter
Compost
Urea
Compost × urea
Total exchange capacity (cmol+/kg)
0.9317
0.8727
0.8756
pH (1 : 1 soil : water)
0.0269
0.1749
0.0595
Organic matter (g/kg)
0.1604
0.0036
0.7973
S (mg/kg)
0.3240
0.4647
0.7185
P (mg/kg)
0.4661
0.2771
0.4179
Ca (mg/kg)
0.4523
0.8386
0.8740
Mg (mg/kg)
0.3694
0.0898
0.6977
K (mg/kg)
0.7658
0.4829
0.0413
Na (mg/kg)
0.2887
0.1182
0.4206
B (mg/kg)
0.2849
0.1422
0.5266
Fe (mg/kg)
0.3824
0.6983
0.1219
Mn (mg/kg)
0.2557
0.2068
0.4542
Cu (mg/kg)
0.1078
0.4458
0.1693
Zn (mg/kg)
0.7921
0.2485
0.0816
Al (mg/kg)
0.0189
0.2622
0.1371
NO3-N (mg/kg)
0.4310
0.4914
0.1622
NH4-N (mg/kg)
0.9296
0.3301
0.0985Table 10
P values for treatment effects on soil chemical properties in 0 to 15 cm and 15 to 25 cm strata for models containing fixed effects of compost (C), urea (U), compost × urea (C × U), and preexperiment soil fertility measurement for each parameter sampled at 15 to 25 cm depth (PSF), with block as a random effect. Each row represents a single model for each soil depth. Significant values at P ≤ 0.05 are in bold.
Parameter
C
U
C × U
PSF
C
U
C × U
PSF
0 to 15 cm depth
15 to 25 cm depth
CEC  (cmol+/kg)
0.2145
0.6271
0.3142
0.0797
0.6827
0.5997
0.4332
0.0363
pH (1 : 1 soil : water)
0.0071
0.1196
0.0705
<0.0001
0.0460
0.1071
0.0856
<0.0001
OM (g/kg)
0.0087
0.6440
0.0505
<0.0001
0.2699
0.0035
0.7938
<0.0001
S (mg/kg)
0.2249
0.9163
0.0720
0.2507
0.8146
0.7345
0.1171
0.0547
P (mg/kg)
0.6723
0.5556
0.3621
0.0157
0.6537
0.3633
0.4554
0.0001
Ca (mg/kg)
<0.0001
0.4688
0.0147
0.0718
0.1873
0.9323
0.6124
0.2071
Mg (mg/kg)
0.0015
0.2469
0.2219
0.0540
0.0542
0.4886
0.4991
0.0006
K (mg/kg)
0.1703
0.3593
0.7202
0.0301
0.2833
0.1041
0.3959
0.3572
Na (mg/kg)
0.0788
0.6333
0.8865
0.0446
0.1636
0.0246
0.0201
0.3319
B (mg/kg)
0.0954
0.4529
0.4883
0.9517
0.0788
0.2146
0.8916
0.5975
Fe (mg/kg)
0.9956
0.4733
0.2342
0.0020
0.4978
0.9109
0.1584
<0.0001
Mn (mg/kg)
0.0002
0.4951
0.6742
0.9859
0.5170
0.2015
0.3686
0.0002
Cu (mg/kg)
0.5569
0.3744
0.5829
0.0343
0.3963
0.8292
0.4546
0.0003
Zn (mg/kg)
0.8383
0.6481
0.2006
0.1849
0.9202
0.5418
0.0771
0.0046
Al (mg/kg)
0.6086
0.4611
0.6980
<0.0001
0.1240
0.2865
0.1456
<0.0001
NO3-N (mg/kg)
0.5608
0.2398
0.2026
0.1510
0.1936
0.4103
0.2641
0.0885
NH4-N (mg/kg)
0.1017
0.6409
0.1306
0.0155
0.9530
0.5630
0.0386
0.0068Greatest N recovery occurred in plots receiving the highest rate of urea (ranging from 5% to 42%) (Table7). Highest overall N recovery was observed with 60 kg N/ha per yr urea × 4 Mg DM/ha per yr compost (79% and 112%, depending on nutritive value period) due to high dry matter yield for this treatment. Farm-to-farm variation explained 38% of residual variation in N recovery for September 2011 samples and 65% for December 2012.


## 4. Discussion

### 4.1. Soil Fertility

Mehlich-3 soil test guidelines for agronomic crops in the subtropics (Florida) suggest medium levels at 26 to 40 mg/kg P, 26 to 40 mg/kg K, and 11 to 23 mg/kg Mg [28]. Although local calibration experiments should be conducted, these results suggest the potential for P and K deficiency in our experiment (medium range), while Mg is sufficiently high. Alternative interpretation guidelines for forage crops in temperate US regions suggest that P, K, and Mg levels are below optimum for forage crops (optimum range is 30 to 50 mg/kg for P, 100 to 200 mg/kg for K, and 120 to 180 mg/kg for Mg) [29]. Variability in soil fertility among farms was high as indicated by large standard deviations for most elements; preexperiment soil nutrient concentrations were higher for high-yielding farms. The interaction of preexperiment soil fertility with main compost and urea effects for OM and some macro- and micronutrients in compost (P, K, Mg, S, Zn, Mn, and Cu) suggests that the ability to maintain soil fertility is dependent on the interaction between soil organic and inorganic amendments and existing soil fertility. These observations support the hypothesis that soils are deficient in multiple nutrients and that compost supplies most soil nutrients but insufficient N, while urea application can address the N shortfall.Soil OM increase during the experiment also took place in plots that did not receive compost, indicating that litter from aboveground and belowground plant biomass may be accumulating over time. Combination of OM from compost and from Mulato II leaf and root residues probably contributed to higher topsoil OM. Compost may play an important role in maintaining or perhaps increasing soil OM over time. Soil Mn was most highly correlated with yield and there were possible Mn deficiencies on some farms with low preexperiment Mn levels (plots at or below 1 mg Mn/kg) that were ameliorated by compost mineralization over time, further supporting the hypothesis that compost addition increases soil fertility status.


### 4.2. Partial Nutrient Balances

Farms represented a range in tropical sandy soils in the region. Preexperiment soil fertility and field histories differed substantially among participating farms. These conditions, combined with variation in rainfall and irrigation, resulted in large yield differences among farms. In general, preexperiment soil fertility was better for the three farms (farms 1, 2, and 3) with the highest yields and most negative N, P, and K balances. However, residual soil nutrients are unlikely to entirely account for the observed negative balances in Arenosols with low CEC (1.3 cmol+/kg with SD ± 0.57) and low organic matter (0.35% with SD ± 0.21) (Table 1). Negative balances suggest that yields will not be sustainable over time even for the highest input levels used in this experiment. For example, DM yield of 20 Mg/ha per yr with 24 g N/kg DM removes 480 kg N/ha per yr through forage mass. Supplying 480 kg N/ha per yr with the cheapest commercial N fertilizer available in Vietnam (urea, 46% N, $0.56 USD/kg in 2013) would require 1043 kg urea at a total cost of $584 USD/ha per yr. This calculation assumes no losses and application at the crop removal rate, assuming limited soil N supply. Supply of other nutrients in addition to N could be achieved using composted cattle manure if applied in sufficient amounts.The 24 Mg DM/ha per yr of compost added in the experiment contained approximately 279 kg N, 66 kg P, 162 kg K, 74 kg Mg, and 240 kg Ca. Maximum N release for compost mineralization combined with urea application at removal rate could be sufficient to support a 20 Mg DM/ha per yr yield with assumed losses of 40% of total N applied. Nutrient removal estimates with a 20 Mg/ha per yr yield (75 kg P/yr, 424 kg K/yr, 70 kg Mg/yr, and 137 kg Ca/yr) suggest that only the supplies of Mg and P were adequate while K supply remained insufficient (−262 kg/ha per yr). Yet a response to added K was not observed in this experiment, suggesting some capacity of the soil to supply K despite low postexperiment soil test levels or that availability of other soil nutrients may have been more limiting than K. Grasses exhibit luxury consumption of K, although yield may remain stable with herbage K concentrations as low as 13 g K/kg DM [30].Nutrient recycling from decaying aboveground and belowground Mulato II litter could supply additional nutrients. In a grazing system withBrachiaria humidicola pastures in Brazil, Boddey et al. [31] discovered litter deposits as high as 170 kg N/ha per yr (30 Mg DM/ha per yr) with low stocking rates. Magnitude of litter contributions is unknown in this experiment, but litter could make a large and important contribution to annual nutrient supply. Further experimentation is necessary in sandy tropical soils to obtain more accurate estimates of nutrient balances.Reasons reported for yield persistence (despite negative balances) during postexperiment discussions with participating farmers include nutrient uptake from buffers and neighboring plots and soil nutrient reserves.Brachiaria spp. grasses are deep-rooted, which can help improve soil properties and transfer nutrients from deeper layers [32]. Assessment of the soil fertility status of deeper soil layers was not done for this study. Additionally, rising water tables during the rainy season may bring dissolved nutrients that could be used by actively growing plants and left behind in soil solution after the rainy season [33]. Furthermore,Brachiaria spp. association with N2 fixing bacteria could supply additional N. Biological N fixation capacity forBrachiaria spp. grasses has been identified using 15N labeling and could supply as much as 20 to 40% of total N uptake (30 to 40 kg N/ha per yr) [34, 35]. A more minor contributor of N is OM. A hectare furrow slice (17 cm deep) contains 2,240,702 kg soil and approximately 11 mg/kg organic-bound N. Approximately 25 kg N/ha per yr could be released from topsoil OM in this study, a small amount compared to the 330 kg N/ha per yr in average crop removal across plots. Atmospheric N deposition could make a small additional contribution on a seasonal basis, perhaps as high as 10 to 15 kg N/ha per yr [36]. Magnitude, and in some cases existence, of these additional contributions is unknown but could explain relatively high Mulato II yields despite negative partial nutrient balances.Partial nutrient balances calculated in this experiment did not account for potential nutrient losses that could make balances even more negative. Leaching losses can occur where compost or urea is applied to sandy soils. Water-logged and flooded plots during the rainy season lend favorable conditions for denitrification losses. High rainfall conditions in sandy soils may induce an annual “reset” of nutrients in soil solution, potentially inhibiting treatment response potential in this experiment. Surface applications of urea and compost (as done in experiment year one) furthermore favor losses from volatilization and runoff.Nitrogen recovery was poor for compost application without urea primarily due to relatively low yields for compost-amended plots. In general, N recoveries were better for higher combined rates of compost and urea. In sandy, nutrient-depleted Zimbabwean soils, similar low N recovery was reported for compost (4% recovery rate) applied to corn at 17 Mg DM/ha per yr (7.8 g N/kg DM) [37]. In the study in Zimbabwe, compost combined with 40 kg N/ha per yr ammonium nitrate top-dressed to corn increased N recovery to 8%. Chikowo et al. [37] concluded that low quality composted cattle manure could not supply adequate N and that most N needs would need to be fulfilled by mineral N. Similar relatively low N recoveries were reported by Lynch et al. [38] for perennial forage production with composted dairy manure applied at 12.8 and 25.6 Mg DM/ha per yr in a temperate system (8.9% to 15.1% recovery). These studies are consistent with our findings that compost is not able to supply sufficient N for forage growth and that N recoveries improve when compost is applied together with inorganic N.


## 4.1. Soil Fertility

Mehlich-3 soil test guidelines for agronomic crops in the subtropics (Florida) suggest medium levels at 26 to 40 mg/kg P, 26 to 40 mg/kg K, and 11 to 23 mg/kg Mg [28]. Although local calibration experiments should be conducted, these results suggest the potential for P and K deficiency in our experiment (medium range), while Mg is sufficiently high. Alternative interpretation guidelines for forage crops in temperate US regions suggest that P, K, and Mg levels are below optimum for forage crops (optimum range is 30 to 50 mg/kg for P, 100 to 200 mg/kg for K, and 120 to 180 mg/kg for Mg) [29]. Variability in soil fertility among farms was high as indicated by large standard deviations for most elements; preexperiment soil nutrient concentrations were higher for high-yielding farms. The interaction of preexperiment soil fertility with main compost and urea effects for OM and some macro- and micronutrients in compost (P, K, Mg, S, Zn, Mn, and Cu) suggests that the ability to maintain soil fertility is dependent on the interaction between soil organic and inorganic amendments and existing soil fertility. These observations support the hypothesis that soils are deficient in multiple nutrients and that compost supplies most soil nutrients but insufficient N, while urea application can address the N shortfall.Soil OM increase during the experiment also took place in plots that did not receive compost, indicating that litter from aboveground and belowground plant biomass may be accumulating over time. Combination of OM from compost and from Mulato II leaf and root residues probably contributed to higher topsoil OM. Compost may play an important role in maintaining or perhaps increasing soil OM over time. Soil Mn was most highly correlated with yield and there were possible Mn deficiencies on some farms with low preexperiment Mn levels (plots at or below 1 mg Mn/kg) that were ameliorated by compost mineralization over time, further supporting the hypothesis that compost addition increases soil fertility status.


## 4.2. Partial Nutrient Balances

Farms represented a range in tropical sandy soils in the region. Preexperiment soil fertility and field histories differed substantially among participating farms. These conditions, combined with variation in rainfall and irrigation, resulted in large yield differences among farms. In general, preexperiment soil fertility was better for the three farms (farms 1, 2, and 3) with the highest yields and most negative N, P, and K balances. However, residual soil nutrients are unlikely to entirely account for the observed negative balances in Arenosols with low CEC (1.3 cmol+/kg with SD ± 0.57) and low organic matter (0.35% with SD ± 0.21) (Table 1). Negative balances suggest that yields will not be sustainable over time even for the highest input levels used in this experiment. For example, DM yield of 20 Mg/ha per yr with 24 g N/kg DM removes 480 kg N/ha per yr through forage mass. Supplying 480 kg N/ha per yr with the cheapest commercial N fertilizer available in Vietnam (urea, 46% N, $0.56 USD/kg in 2013) would require 1043 kg urea at a total cost of $584 USD/ha per yr. This calculation assumes no losses and application at the crop removal rate, assuming limited soil N supply. Supply of other nutrients in addition to N could be achieved using composted cattle manure if applied in sufficient amounts.The 24 Mg DM/ha per yr of compost added in the experiment contained approximately 279 kg N, 66 kg P, 162 kg K, 74 kg Mg, and 240 kg Ca. Maximum N release for compost mineralization combined with urea application at removal rate could be sufficient to support a 20 Mg DM/ha per yr yield with assumed losses of 40% of total N applied. Nutrient removal estimates with a 20 Mg/ha per yr yield (75 kg P/yr, 424 kg K/yr, 70 kg Mg/yr, and 137 kg Ca/yr) suggest that only the supplies of Mg and P were adequate while K supply remained insufficient (−262 kg/ha per yr). Yet a response to added K was not observed in this experiment, suggesting some capacity of the soil to supply K despite low postexperiment soil test levels or that availability of other soil nutrients may have been more limiting than K. Grasses exhibit luxury consumption of K, although yield may remain stable with herbage K concentrations as low as 13 g K/kg DM [30].Nutrient recycling from decaying aboveground and belowground Mulato II litter could supply additional nutrients. In a grazing system withBrachiaria humidicola pastures in Brazil, Boddey et al. [31] discovered litter deposits as high as 170 kg N/ha per yr (30 Mg DM/ha per yr) with low stocking rates. Magnitude of litter contributions is unknown in this experiment, but litter could make a large and important contribution to annual nutrient supply. Further experimentation is necessary in sandy tropical soils to obtain more accurate estimates of nutrient balances.Reasons reported for yield persistence (despite negative balances) during postexperiment discussions with participating farmers include nutrient uptake from buffers and neighboring plots and soil nutrient reserves.Brachiaria spp. grasses are deep-rooted, which can help improve soil properties and transfer nutrients from deeper layers [32]. Assessment of the soil fertility status of deeper soil layers was not done for this study. Additionally, rising water tables during the rainy season may bring dissolved nutrients that could be used by actively growing plants and left behind in soil solution after the rainy season [33]. Furthermore,Brachiaria spp. association with N2 fixing bacteria could supply additional N. Biological N fixation capacity forBrachiaria spp. grasses has been identified using 15N labeling and could supply as much as 20 to 40% of total N uptake (30 to 40 kg N/ha per yr) [34, 35]. A more minor contributor of N is OM. A hectare furrow slice (17 cm deep) contains 2,240,702 kg soil and approximately 11 mg/kg organic-bound N. Approximately 25 kg N/ha per yr could be released from topsoil OM in this study, a small amount compared to the 330 kg N/ha per yr in average crop removal across plots. Atmospheric N deposition could make a small additional contribution on a seasonal basis, perhaps as high as 10 to 15 kg N/ha per yr [36]. Magnitude, and in some cases existence, of these additional contributions is unknown but could explain relatively high Mulato II yields despite negative partial nutrient balances.Partial nutrient balances calculated in this experiment did not account for potential nutrient losses that could make balances even more negative. Leaching losses can occur where compost or urea is applied to sandy soils. Water-logged and flooded plots during the rainy season lend favorable conditions for denitrification losses. High rainfall conditions in sandy soils may induce an annual “reset” of nutrients in soil solution, potentially inhibiting treatment response potential in this experiment. Surface applications of urea and compost (as done in experiment year one) furthermore favor losses from volatilization and runoff.Nitrogen recovery was poor for compost application without urea primarily due to relatively low yields for compost-amended plots. In general, N recoveries were better for higher combined rates of compost and urea. In sandy, nutrient-depleted Zimbabwean soils, similar low N recovery was reported for compost (4% recovery rate) applied to corn at 17 Mg DM/ha per yr (7.8 g N/kg DM) [37]. In the study in Zimbabwe, compost combined with 40 kg N/ha per yr ammonium nitrate top-dressed to corn increased N recovery to 8%. Chikowo et al. [37] concluded that low quality composted cattle manure could not supply adequate N and that most N needs would need to be fulfilled by mineral N. Similar relatively low N recoveries were reported by Lynch et al. [38] for perennial forage production with composted dairy manure applied at 12.8 and 25.6 Mg DM/ha per yr in a temperate system (8.9% to 15.1% recovery). These studies are consistent with our findings that compost is not able to supply sufficient N for forage growth and that N recoveries improve when compost is applied together with inorganic N.


## 5. Conclusions

Field experiment results suggest that the ability to sustain soil fertility over time in sandy tropical soils is dependent on the interaction between existing soil fertility and soil organic and inorganic amendments. Negative partial nutrient balances for N, P, and K indicate that, for the compost and urea application rates used in this experiment with actively growing forage grass, soil degradation is occurring and yields are unlikely to be sustainable over time. Overall, compost plays a potentially important role in maintaining OM, preventing acidification, and supplying key macro- and micronutrients for plant uptake. Urea supplies readily available N, which the grass could benefit from once other nutrient limitations had been overcome (with compost application). More broadly, these results suggest that extremely high rates of nutrient additions are required to realize the high yield potential in tropical forage systems. The consequences of high required nutrient inputs have important implications for intensification of smallholder crop-livestock systems and resulting nutrient loss potential.



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*Source: 4709024-2016-03-03.xml*