# Vibrational Investigations of Silver-Doped Hydroxyapatite with Antibacterial Properties

Authors: Carmen Steluta Ciobanu; Simona Liliana Iconaru; Phillippe Le Coustumer; Daniela Predoi
Journal: Journal of Spectroscopy
 (2013)

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## Abstract

Silver-doped hydroxyapatite (Ag:HAp) was obtained by coprecipitation method. Transmission electron microscopy (TEM), infrared, and Raman analysis confirmed the development of Ag:HAp with good crystal structure. Transmission electron microscopy analysis showed an uniform ellipsoidal morphology with particles from 5 nm to 15 nm. The main vibrational bands characteristic to HAp were identified. The bands assigned to  phosphate vibrational group were highlighted in infrared and Raman spectra. The most intense peak   Raman spectrum is the narrow band observed at 960 cm−1. In this article Ag:HAp-NPs were also evaluated for their antimicrobial activities against gram-positive, gram-negative, and fungal strains. The specific antimicrobial activity revealed by the qualitative assay demonstrates that our compounds are interacting differently with the microbial targets.


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## Body

## 1. Introduction

Nanotechnology is an emerging field which reaches out to develop a bridge between the macroscopic and the atomic or molecular levels of matter. Nowadays the research community looks for answers in the field of nanomaterials for the most pressing problems in medicine, electronics, optics, and environment. The most studied materials are the ones showing similarities and good compatibility with the living tissues due to the large medical applications they qualify for [1–3]. Biomaterials, especially bioceramics belonging to calcium phosphate class, are widely investigated because of their unique properties and high similarities with human osseous tissue. In the last decade a lot of attention was directed towards hydroxyapatite (HAp) from the family of apatites, which is known to be in its natural form a major inorganic constituent of human bones and teeth [4–6]. Having the general formula, Ca10 (PO4)6 (OH)2, HAp has been widely studied for dental and orthopedic applications due to its outstanding properties of osteoconductivity and biocompatibility. In order to be able to meet the high demands that are nowadays required in the field of medical implants, dental implants, or bone tissue reconstruction, biomaterials such as HAp are often improved with other significant elements [7–9]. The HAp structure allows the substitution of Ca2+ ions with other metal ions such as Zn2+, Cu2+, and Ag+ [10]. As we all know, silver has been widely used since ancient times as an antimicrobial agent. Due to the outbreak of diseases and apparition of drug resistant bacterial strains, in the last years, there have been made extensive studies in order to develop new antibacterial compounds based on the effects of silver against bacterial strains. The antibacterial action of silver nanoparticles is not completely understood and in pure state it has been reported to be toxic towards human organism [11].The result of substituting Ca2+ ions with Ag+ ions in the structure of HAp is a new compound which presents high biocompatibility, osteoconductivity, bone regeneration properties, and above all good antibacterial properties [12].This work presents the results of infrared spectroscopic analysis and Raman investigations of silver-doped hydroxyapatite withxAg = 0, 0.4, and 0.5. This study represents an extension of results reported in our previous work [13]. The aim of this study was the evaluation of the antibacterial activity of Ag:HAp nanoparticles when the silver concentration in the samples was increased at 0.4 and 0.5. The structure and morphology of the obtained samples were characterized by transmission electron microscopy (TEM). In vitro qualitative antimicrobial activity of silver-doped hydroxyapatite samples was also investigated.


## 2. Experimental Section

The synthesis of (Ca10-xAgx)(PO4)6(OH)2, with xAg = 0, xAg = 0.4 and xAg = 0.5 was carried out as reported in other papers [13]. Transmission electron microscopy (TEM) studies were carried out using an FEI Tecnai 12 equipped with a low-dose digital camera from Gatan. The specimen for TEM imaging was prepared by ultramicrotomy in order to obtain thin section of about 60 nm. The powder is embedded in an epoxy resin (polaron 612) before microtomy. TEM modes used were bright field (BF) and selected area diffraction (SAD). The functional groups present in the prepared nanoparticles and thin films were identified by FTIR using a spectrum BX spectrometer. To obtain the nanoparticles spectra, 1% of the nanopowder was mixed and grounded with 99% KBr. Tablets of 10 mm diameter were prepared by pressing the powder mixture at a load of 5 tons for 2 min. The spectrum was recorded in the range of 500 to 4000 cm−1 with 4 cm−1 resolution. Micro-Raman spectra on powders were performed in a backscattering geometry at room temperature and in ambient air, under a laser excitation wavelength of 514 nm, using a Jobin Yvon T64000 Raman spectrophotometer under a microscope.The microbial strains identification was confirmed by aid of VITEK II automatic system. VITEK cards for identification and susceptibility testing were inoculated and incubated according to the manufacturer’s recommendations. The results were interpreted using the software version AMS R09.1. Microbial suspensions of1.5×108 CFU/mL corresponding to 0.5 McFarland density obtained from 15–18 h bacterial cultures developed on solid media were used in our experiments. The tested substances were solubilised in DMSO and the starting stock solution was of 5000 μg/mL concentration. The qualitative screening was performed by an adapted disk diffusion method [14–18].


## 3. Results and Discussions

Figure1 exhibits the TEM images of pure HAp (xAg = 0) and Ag:HAp (xAg = 0.4, and xAg = 0.5) with low resolution. As shown in Figure 1, pure HAp and Ag:HAp exhibit an ellipsometric morphology, which is consistent with the SEM results [13].Figure 1
The TEM images of pure HAp (xAg = 0) and Ag:HAp (xAg = 0.4 and xAg = 0.5) with low resolution.It can be seen from the HRTEM image of Ag:HAp (Figure2(a)) that the crystalline phase of hydroxyapatite with well-resolved lattice fringes can be observed. The distances between the adjacent lattice fringes (2.72 Å) agree well with the d300 spacing of the literature values (2.872 Å) (ICDD-PDF no. 9-432). All 3 samples exhibit a uniform ellipsoidal morphology with particles from 5 nm to 15 nm. The Figure 2(b) shows a selected area electron diffraction (SAED) pattern recorded from an area containing a large number of ellipsometric nanoparticles. The rings in the SAED pattern can be indexed as (002), (210), (211), (310), (222), (213), (004) and (304) reflections of the hexagonal HAp, in agreement with the XRD results. No extra reflections are observed and we can therefore conclude that the product consists of pure HAp ellipsometric nanoparticles.HRTEM image and SAED analysis of Ag:HAp withxAg = 0.5.
(a)
(b)These results are well consistent with the XRD results [13], revealing that the doping components have little influence on the surface morphology of the samples. The morphology identifications indicated that the nanoparticles with good crystal structure could be made by coprecipitation method at low temperature.FT-IR spectroscopy was performed in order to investigate the functional groups present in nanohydroxyapatite,Ca10-xAgx(PO4)6(OH)2, (xAg = 0, 0.4, and 0.5) synthesized at 100°C by coprecipitation method. Figure 3 shows the FT-IR results obtained from Ag:HAp-NPs when the xAg increases from 0 to 0.5. The absorption peak in the region of 1600–1700 cm−1, ascribed to O–H bending mode, is evidence of the presence of absorbed water in the synthesis products [19–21].Figure 3
Transmittance infrared spectra of the Ag:HAp samples synthesized withxAg = 0, 0.4, and 0.5.These data clearly revealed the presence of various vibrational modes corresponding to phosphate and hydroxyl groups. For all the samples the presence of strong OH vibration peak could be noticed. The peak observed at 634 cm−1 is attributed to the characteristic stretching and vibrational modes of structural OH groups [22, 23]. The band at 1630 cm−1 corresponds to the adsorbed H2O [23].Bands characteristics ofPO43-  tetrahedral apatite’s structure are clearly observed at 473 cm−1, 563 cm−1, 603 cm−1, 960 cm-1, and 1095–1033 cm−1 [24, 25]. The peak at 473 cm−1 is attributed to PO4ν2. The peaks at 563 cm−1 and 603 cm−1 belong to PO4ν4. The peak at 960 cm−1 is attributed to PO4ν1 and the peaks at 1095–1033 cm−1,  are attributed to  PO4ν3. A CO32- band occurred in the spectra at 1452 and 1408 cm−1 [26, 27]. The bands at 875 cm−1 are attributed to HPO42- ions [28–30].Complementary information can be obtained from Raman spectroscopy. Raman spectra of Ag:HAp from 1200 cm−1 to 400 cm−1 is shown in Figure 4. The OH- vibrational bands expected in the region of 630 cm−1 are not clearly detected. This behavior is in good accord with the previous studies [31].Figure 4
Raman spectra of the Ag:HAp samples synthesized withxAg = 0, 0.4, and 0.5.We assigned the bands present at 1026 cm−1 (ν3), 1047 cm−1 (ν3), and 1073 cm−1 (ν3) to the asymmetric ν3 (P–O) stretching. The internal modes of the PO43- tetrahedral ν1 frequency (960 cm−1) corresponds to the symmetric stretching of P–O bonds. The 616 cm−1, 590 cm−1, and 576 cm−1 bands arise from ν4 PO4 [28]. The vibrational bands at 446 cm−1 (ν2) and 431 cm−1 (ν2) are attributed to the O–P–O bending modes. The band at 1070 cm−1 (ν1) attributed to CO32- impurity is by the strong intensity PO4 band at 1073 cm−1. The other CO3 modes ν3, ν4, and ν2 have band positions not obscured by the PO4 bands, but they have weak intensities and were not detected [32]. Water vibrational modes give rise to weak intensity stretching and bending bands in Raman spectra. These water bands, expected at about the same wave number in FTIR spectra, were not observed in Raman spectra.The qualitative antimicrobial activity of the tested compounds performed using stock solutions of 5 mg/mL obtained in dimethylsulfoxide (DMSO) allowed the selection of the active compounds, by the occurrence of high inhibition zones around the spotted compound. The results of the qualitative screening of the antimicrobial activity of Ag:HAp compounds against differentgram-positive and gram-negative bacteria, as well as fungal strains are presented in Table 1. The tested compounds proved to be very active on C. kruseistrain, irrespective of the Ag concentration. On the other hand, our tests proved them to be also active on P. aeruginosa1397, S. aureus0364, K. pneumoniae2968, and E. cloacae61R. The tested compounds, at certain concentrations, were also active against E. coli 714, E. coli ATCC 25922, and B. subtilis.Table 1
The results of the qualitative screening of the antimicrobial activity of Ag:HAp compounds against differentgram-positive and gram-negative bacteria, as well as fungal strains.
Microbial strains
x
Ag = 0
x
Ag = 0.4
x
Ag = 0.5
Gram-negative strains
Enterobacteriaceae
family
E.  coli ATCC 25922
−
+
±
E.  coli 714
−
−
±
K.  pneumoniae 2968
−
+
+
E.  cloacae 61R
−
+
+
Pseudomonadaceae
family
P.  aeruginosa 1397
±
+
±
Gram-positive strains
Bacillaceaefamily
B.  subtilis
−
±
±
Streptococcaceaefamily
E.  faecalis ATCC 29212
−
−
−
Micrococcaceaefamily
S.  aureus 0364
−
+
+
Fungal strain
C.  krusei 963
+
+
+For the samples withxAg=0 the antibacterial activity was not present in the case of E. faecalis ATCC 29212, B. subtilis, E. coli ATCC 25922, K. Pneumoniae 2968, E. cloacae 61R,and S. aureus 0364 bacterial strains.On the other hand it showed an inhibitory effect on the fungal strainC. krusei 963and thegram-negative strain P. aeruginosa 1397.ForxAg=0.4, the tested samples presented a good antibacterial activity both ongram-positive, gram-negative,and fungal strains,exceptingE. coli 714 and E. faecalis ATCC 29212.The samples withxAg=0.5 had a good antibacterial effect for all the tested microbial strains, except for E. faecalis ATCC29212.


## 4. Conclusions

Ag:HAp nanoparticles withxAg = 0, 0.4, and 0.5 were prepared by coprecipitation method at low temperature and characterized by TEM, FT-IR, and FT-Raman. TEM investigations confirmed the nanocrystalline nature of the synthesized powder and the ellipsometric morphology of the Ag:HAp particles. All 3 samples exhibit a uniform ellipsoidal morphology with particles from 5 nm to 15 nm. Bands characteristics of PO43- tetrahedral apatite’s structure are clearly observed by infrared and Raman analysis. The results of the qualitative screening of the antimicrobial activity of Ag:HAp compounds against different gram-positive and gram-negative bacterial strains proved to be very good irrespective of the Ag concentration. The specific antimicrobial activity revealed by the qualitative assay demonstrates that our compounds interacted differently with the microbial targets.



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*Source: 471061-2012-08-15.xml*