# TAFRO Syndrome Associated with EBV and Successful Triple Therapy Treatment: Case Report and Review of the Literature

Authors: Malorie Simons; Emmanuel Apor; James N. Butera; Diana O. Treaba
Journal: Case Reports in Hematology
 (2016)

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## Abstract

TAFRO syndrome is a rare constellation of symptoms: thrombocytopenia, anasarca, reticulin fibrosis of the bone marrow, renal dysfunction, and organomegaly. Its pathogenesis involves an excessive and inappropriate cytokine storm, most notably from IL-6, causing multiorgan failure; however, its etiology is undetermined. Starting in 2012, TAFRO syndrome was first identified in Japan as an atypical variant of Castleman’s disease. Previous reports include various different treatment protocols with inconsistent survival outcomes. Here we report the first known American, EBV positive but HIV and HHV-8 negative, male with TAFRO syndrome. He was successfully treated with an unusual three-drug regimen including tocilizumab, etoposide, and rituximab. We review the literature of TAFRO syndrome, discuss its possible viral etiology, and propose an original treatment regimen.


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## Body

## 1. Introduction

An extremely rare constellation of symptoms, the TAFRO syndrome includes thrombocytopenia, anasarca, reticulin fibrosis of the bone marrow, renal dysfunction, and organomegaly. In 2012, TAFRO syndrome was first identified in Japan as an atypical variant of multicentric Castleman disease (MCD), a group of disorders associated with systemic inflammatory symptoms, reactive proliferation of benign lymphocytes, and multiple organ impairment secondary to proinflammatory cytokines [1]. To date, there have been up to 25 reports of TAFRO syndrome, most of which were in Japan, with different treatment protocols and survival outcomes.The etiology of TAFRO syndrome is unclear, although it is reasonable to propose that there is an insult to the immune system that produces hypercytokinemia. Human herpes virus-8 (HHV-8) is a known cause of this cytokine storm in HIV positive MCD patients and some HIV negative patients [2]. In addition, Epstein-Barr virus has also been associated with Castleman disease [3, 4]. However, to date, there is no known cause of TAFRO syndrome.Due to the rarity of TAFRO syndrome, there are no set guidelines on treatment although leading experts are beginning a multicenter retrospective clinical study to start this process [5]. Previous treatment efforts have centered on anticytokine therapy, namely, Interleukin-6 (IL-6) inhibition, in combination with immunosuppressive therapy with variable success.Here we report a case of an American male with biopsy proven EBV positive TAFRO syndrome, who was successfully treated with therapies focused on cytokine reduction (tocilizumab), immunosuppression (rituximab), and cytotoxicity (etoposide). His case may help elucidate a possible viral etiology for the disease and also demonstrates a novel, successful treatment regimen that dampened the hypercytokinemia response which is uniformly seen in TAFRO patients and ultimately normalized this patient’s symptoms and organ dysfunction.


## 2. Case Presentation

### 2.1. Clinical Presentation

A 22-year-old American male presented to an outside hospital with an initial complaint of pleuritic chest pain, shortness of breath, and lightheadedness. Prior to the admission, he had two weeks of fevers, night sweats, and malaise and one week of constipation, nausea, and abdominal discomfort. Vital signs were significant for fever, tachycardia, tachypnea, and hypoxia. Physical exam was notable for a diaphoretic, well-nourished male with bilateral lower lung crackles, hepatosplenomegaly, and bilateral axillary lymphadenopathy. Laboratory studies were remarkable for acute renal failure (creatinine 2.28 mg/dL, reference range 0.6–1.3 mg/dL, leukocytosis (WBC) 37 × 109/L, and reference range: 3.5–11 × 109/L). Computed tomography demonstrated diffuse axillary lymphadenopathy (1 to 1.2 cm in diameter), hepatomegaly, and anasarca (Figures 1(a) and 1(b)). He was then transferred to Rhode Island Hospital, where he progressed to severe thrombocytopenia (platelets count 2 × 109/L), anemia (hemoglobin 7.7 g/dL), anuric renal failure with hyperuricemia, hyperkalemia, hypocalcemia, and hyperphosphatemia requiring emergent dialysis, and hypoxic respiratory failure necessitating intubation.Figure 1
Computed tomography demonstrating diffuse axillary lymphadenopathy measuring 1 to 1.2 cm in short axis (a) and ascites (b).
(a)
(b)


### 2.2. Diagnostic Course

Biopsy of the left axillary lymph nodes was remarkable for scattered secondary lymphoid follicles variable in size, some with well-developed germinal centers with round to ovoid to slightly irregular contours to a few secondary lymphoid follicles with small atrophic germinal centers (Figures2(a) and 2(b)) depleted of B lymphoid cells (Figure 2(c), PAX-5 antibody) and with very low proliferation rates (Figure 2(d), MIB-1 antibody). There was interfollicular polytypic plasmacytosis, composed mostly of mature-appearing MUM1 positive plasma cells (Figure 3(a)) associated with a high proliferation rate (Figure 3(b), MIB-1 antibody), and there was a subset of lymphoid cells in the interfollicular region with predominant cytoplasmic positivity for EBER in situ hybridization (Figure 3(c)). By flow cytometry immunophenotypic analysis identified a larger CD3 positive T-cell population (77% of the lymphoid cells analyzed) in the lymph node sample examined with a CD4-to-CD8 cell ratio of 4.3 : 1 with partial loss of CD7 in a small subset of the T-cells analyzed. The smaller (21% of the lymphoid cells analyzed) CD19 positive B lymphoid population was polytypic with a surface immunoglobulin kappa to surface immunoglobulin lambda of 1.3 and 1% NK cells were also identified. Polymerase chain reaction (PCR) studies did not detect immunoglobulin heavy chain gene rearrangements or T-cell receptor beta and gamma gene rearrangements but detected the presence of EBV DNA sequences (Figure 4(a)). The above findings are in keeping with an atypical lymphoid hyperplasia with features of a mixed type of Castleman disease.Figure 2
Biopsy of left axillary lymph node demonstrating (a) well-developed germinal centers with round to ovoid to slightly irregular contours, (b) small atrophic germinal centers, (c) depleted of PAX-5 positive B lymphoid cells with very low proliferation (d) rates using MIB-1 antibody.
(a)
(b)
(c)
(d)Figure 3
Biopsy of left axillary lymph node, with increased numbers of MUM1 positive plasma cells in the interfollicular areas ((a) objective 50x) and with a high proliferation rate ((b) MIB-1 antibody, objective 500x). EBER in situ hybridization stain with increased subset of positive cells in the interfollicular area ((c) objective 500x).
(a)
(b)
(c)Figure 4
PCR gel for detection of EBV DNA: samples 1 and 2 are two replicates of patient’s DNA with different amounts of sample; M is the 100 bp ladder molecular weight marker. PC, positive control; NC, negative control; NTC, no template control (a). Hypercellular bone marrow biopsy ((b) hematoxylin and eosin stain 50x, immersion oil) and aspirate ((c) Wright Giemsa stain, 100x, immersion oil) with trilineage hematopoiesis; a small hypolobated megakaryocyte ((c) inset).
(a)
(b)
(c)Patient’s bone marrow examination was remarkable for hypercellularity with trilineage hematopoiesis (Figure4(b)) with mild dyserythropoiesis (Figure 4(c)) and dysmegakaryopoiesis (Figure 4(c) inset) but was associated with reported normal 46, XY karyotype. There was mild increase in reticulin deposition in patient’s bone marrow. Of interest, flow cytometry analysis of the peripheral blood identified a drastic reduction of the CD4 positive T-cell subset with absolute numbers of CD8 positive T-cells, B-cells, and NK cells within the normal reference ranges. The CD4-to-CD8 cell ratio was reversed (0.37 : 1). Flow cytometry analysis of his ascitic fluid identified a predominant CD3+ T-cell population (90% of the analyzed lymphoid cells) with a reversed CD4-to-CD8 cell ratio (0.25 : 1).Overall, based on the constellation of clinical and pathological data the patient was diagnosed with TAFRO syndrome. Additional work-up for TAFRO syndrome included an elevated IL-2R of 8944 (reference range 622–1619 pg/mL), IL-6 596 (reference range 0.31–5 pg/mL), and CRP 30 (reference range 0–10 mg/ml).


### 2.3. Treatment Course and Outcome

Upon the diagnosis of TAFRO syndrome, the patient was initially started on sole anti-IL-6 therapy with tocilizumab, 8 mg/kg every 2 weeks. Due to worsening clinical status he was then treated with weekly rituximab 375 mg/m2 and etoposide 100 mg/m2 (dose reduced 50% due to renal failure). Following this regimen, the patient showed drastic clinical improvement in symptoms, laboratory findings, and imaging. He was followed up as an outpatient where he completed an eight-week, weekly course of tocilizumab, concomitantly with a four-week course of weekly etoposide and rituximab. By the eighth week, his anasarca, organomegaly, and lymphadenopathy resolved and his renal function normalized. In addition, his complete blood count normalized, IL-6 decreased to 123 pg/mL, and CRP normalized to less than 20 mg/ml. He remains symptom-free from the disease after almost 2 years from the diagnosis.


## 2.1. Clinical Presentation

A 22-year-old American male presented to an outside hospital with an initial complaint of pleuritic chest pain, shortness of breath, and lightheadedness. Prior to the admission, he had two weeks of fevers, night sweats, and malaise and one week of constipation, nausea, and abdominal discomfort. Vital signs were significant for fever, tachycardia, tachypnea, and hypoxia. Physical exam was notable for a diaphoretic, well-nourished male with bilateral lower lung crackles, hepatosplenomegaly, and bilateral axillary lymphadenopathy. Laboratory studies were remarkable for acute renal failure (creatinine 2.28 mg/dL, reference range 0.6–1.3 mg/dL, leukocytosis (WBC) 37 × 109/L, and reference range: 3.5–11 × 109/L). Computed tomography demonstrated diffuse axillary lymphadenopathy (1 to 1.2 cm in diameter), hepatomegaly, and anasarca (Figures 1(a) and 1(b)). He was then transferred to Rhode Island Hospital, where he progressed to severe thrombocytopenia (platelets count 2 × 109/L), anemia (hemoglobin 7.7 g/dL), anuric renal failure with hyperuricemia, hyperkalemia, hypocalcemia, and hyperphosphatemia requiring emergent dialysis, and hypoxic respiratory failure necessitating intubation.Figure 1
Computed tomography demonstrating diffuse axillary lymphadenopathy measuring 1 to 1.2 cm in short axis (a) and ascites (b).
(a)
(b)


## 2.2. Diagnostic Course

Biopsy of the left axillary lymph nodes was remarkable for scattered secondary lymphoid follicles variable in size, some with well-developed germinal centers with round to ovoid to slightly irregular contours to a few secondary lymphoid follicles with small atrophic germinal centers (Figures2(a) and 2(b)) depleted of B lymphoid cells (Figure 2(c), PAX-5 antibody) and with very low proliferation rates (Figure 2(d), MIB-1 antibody). There was interfollicular polytypic plasmacytosis, composed mostly of mature-appearing MUM1 positive plasma cells (Figure 3(a)) associated with a high proliferation rate (Figure 3(b), MIB-1 antibody), and there was a subset of lymphoid cells in the interfollicular region with predominant cytoplasmic positivity for EBER in situ hybridization (Figure 3(c)). By flow cytometry immunophenotypic analysis identified a larger CD3 positive T-cell population (77% of the lymphoid cells analyzed) in the lymph node sample examined with a CD4-to-CD8 cell ratio of 4.3 : 1 with partial loss of CD7 in a small subset of the T-cells analyzed. The smaller (21% of the lymphoid cells analyzed) CD19 positive B lymphoid population was polytypic with a surface immunoglobulin kappa to surface immunoglobulin lambda of 1.3 and 1% NK cells were also identified. Polymerase chain reaction (PCR) studies did not detect immunoglobulin heavy chain gene rearrangements or T-cell receptor beta and gamma gene rearrangements but detected the presence of EBV DNA sequences (Figure 4(a)). The above findings are in keeping with an atypical lymphoid hyperplasia with features of a mixed type of Castleman disease.Figure 2
Biopsy of left axillary lymph node demonstrating (a) well-developed germinal centers with round to ovoid to slightly irregular contours, (b) small atrophic germinal centers, (c) depleted of PAX-5 positive B lymphoid cells with very low proliferation (d) rates using MIB-1 antibody.
(a)
(b)
(c)
(d)Figure 3
Biopsy of left axillary lymph node, with increased numbers of MUM1 positive plasma cells in the interfollicular areas ((a) objective 50x) and with a high proliferation rate ((b) MIB-1 antibody, objective 500x). EBER in situ hybridization stain with increased subset of positive cells in the interfollicular area ((c) objective 500x).
(a)
(b)
(c)Figure 4
PCR gel for detection of EBV DNA: samples 1 and 2 are two replicates of patient’s DNA with different amounts of sample; M is the 100 bp ladder molecular weight marker. PC, positive control; NC, negative control; NTC, no template control (a). Hypercellular bone marrow biopsy ((b) hematoxylin and eosin stain 50x, immersion oil) and aspirate ((c) Wright Giemsa stain, 100x, immersion oil) with trilineage hematopoiesis; a small hypolobated megakaryocyte ((c) inset).
(a)
(b)
(c)Patient’s bone marrow examination was remarkable for hypercellularity with trilineage hematopoiesis (Figure4(b)) with mild dyserythropoiesis (Figure 4(c)) and dysmegakaryopoiesis (Figure 4(c) inset) but was associated with reported normal 46, XY karyotype. There was mild increase in reticulin deposition in patient’s bone marrow. Of interest, flow cytometry analysis of the peripheral blood identified a drastic reduction of the CD4 positive T-cell subset with absolute numbers of CD8 positive T-cells, B-cells, and NK cells within the normal reference ranges. The CD4-to-CD8 cell ratio was reversed (0.37 : 1). Flow cytometry analysis of his ascitic fluid identified a predominant CD3+ T-cell population (90% of the analyzed lymphoid cells) with a reversed CD4-to-CD8 cell ratio (0.25 : 1).Overall, based on the constellation of clinical and pathological data the patient was diagnosed with TAFRO syndrome. Additional work-up for TAFRO syndrome included an elevated IL-2R of 8944 (reference range 622–1619 pg/mL), IL-6 596 (reference range 0.31–5 pg/mL), and CRP 30 (reference range 0–10 mg/ml).


## 2.3. Treatment Course and Outcome

Upon the diagnosis of TAFRO syndrome, the patient was initially started on sole anti-IL-6 therapy with tocilizumab, 8 mg/kg every 2 weeks. Due to worsening clinical status he was then treated with weekly rituximab 375 mg/m2 and etoposide 100 mg/m2 (dose reduced 50% due to renal failure). Following this regimen, the patient showed drastic clinical improvement in symptoms, laboratory findings, and imaging. He was followed up as an outpatient where he completed an eight-week, weekly course of tocilizumab, concomitantly with a four-week course of weekly etoposide and rituximab. By the eighth week, his anasarca, organomegaly, and lymphadenopathy resolved and his renal function normalized. In addition, his complete blood count normalized, IL-6 decreased to 123 pg/mL, and CRP normalized to less than 20 mg/ml. He remains symptom-free from the disease after almost 2 years from the diagnosis.


## 3. Discussion

TAFRO syndrome, an atypical variant of Castleman disease, is a rare constellation of symptoms that has only recently been described.


### 3.1. Viral Etiology for TAFRO Syndrome

The patient described in this case report was positive for EBV DNA sequences by PCR in both the biopsied lymph nodes and bone marrow sample and had a subset of positive lymphoid cells by EBER on in situ hybridization. To date, no known virus has been associated with TAFRO syndrome; however, certain viruses are associated with MCD, namely, HHV-8 in HIV positive patients and EBV. This is a novel finding and may be a contributor to the etiology of TAFRO syndrome. In addition, the lymph node findings with a marked discrepancy between the low proliferation rate noted in atrophic germinal centers depleted of B lymphoid cells and the morphologically mature-appearing plasma cell proliferation noted in the interfollicular regions, associated with a high proliferation rate, may underline an extreme immune response leading to exhaustion of the germinal center cells and differentiation toward plasma cells secondary to strong antigenic stimulation. Furthermore, the reversed CD4-to-CD8 T-cell ratio identified in the peripheral blood and ascitic fluid in our patient, which initially raised in the differential diagnosis a possible HIV infection, has been noted in patients with other acute viral infections such as EBV and CMV, supporting also the possibility of an EBV driven TAFRO syndrome [6].


### 3.2. Treatment

Due to the rarity of TAFRO syndrome there are no guidelines to treatment. Also, the ambiguity of presenting symptoms and findings complicates treatment directions. In review of previous TAFRO cases, using PubMed search terms for confirmed TAFRO diagnosis, patterns of treatment can be seen (Table1). Most cases involved treatment with steroids [7–13]. Unfortunately, symptomatic improvement gained from steroids alone has usually been reported as short-lived [2, 14].Table 1
Summary of the most common treatment regimens for those diagnosed with TAFRO syndrome.
Case report
Age (years)/gender
TAFRO-directed treatment
General outcome
1st line
2nd line
3rd line
Simons, 2016
22/M
TCZ
TCZ, rituximab, etoposide
Clinical improvement
Tedesco, 2015
21/F
Prednisone, TCZ
Rituximab, cyclophosphamide, vincristine, prednisone
Clinical improvement
Takai et al., 2010 [17]
39/M
Methylprednisolone, prednisolone
Clinical improvement
38/M
Prednisolone
Clinical improvement
Koduri et al., 2014 [15]
14/M
CHOP× 6
Thalidomide
Persistent lymphadenopathy, no edema or ascites
Kubokawa et al., 2014 [7]
15/M
Methylprednisolone
TCZ, IVIG
Cytokine levels decreased by 4 months
Ozawa et al., 2014 [10]
48/F
IVIG
Steroids
Rituximab
Resolution of thrombocytopenia and marrow fibrosis
Kawabata et al., 2013 [9]
47/F
Prednisone
TCZ, steroids
Improvement of IL-6 levels, no signs of disease recurrence
Inoue et al., 2013 [8]
49/F
Dexamethasone
Cyclosporine A
Resolution of thrombocytopenia and marrow fibrosis
Iwaki et al., 2013 [11]
43/F
Methylprednisolone
Rituximab, TCZ, steroids
Improvement of thrombocytopenia and anasarca
Masaki et al., 2013 [12]
57/F
Methylprednisolone
CHOEP
Died from septic shock
73/M
Prednisolone
Died from multiorgan failure
Takai et al., 2010 [17]
47/F
CHOP, prednisolone
Prednisolone (maintenance)
Clinical improvement
56/M
Methylprednisolone, IVIG
Cyclosporin A
Improved platelet count, edema, ascites
49/M
Methylprednisolone, IVIG
Died from multiorgan failure
53/F
Prednisolone
Prednisolone, cyclophosphamide
Clinical improvement
56/F
Methylprednisolone, cyclosporin A
Clinical improvement
Abdo et al., 2014 [18]
81/M
Prednisone
Clinical improvementIn several cases, immunotherapeutic agents were utilized. Rituximab, a monoclonal antibody directed against the CD20 antigen on B-lymphocytes, has shown durable responses in HIV positive MCD [14]. Two cases of TAFRO syndrome reported clinical improvement with rituximab [8, 10]. In one case, it was used as monotherapy and, in the other, rituximab was used in combination with methylprednisolone and tocilizumab. Cyclosporin A was documented to have resulted in complete remission in four cases [8, 13].Ideally chemotherapy would provide a means to eliminate large portions of hypercytokine-secreting cells; however, its use without immunosuppressive therapy has not had lasting and meaningful results [12, 15]. Only one patient in the literature [16] had been treated with regimen consistent of rituximab, tocilizumab, cyclophosphamide, and etoposide.Since elevations in IL-6 have been thought to contribute to the pathophysiology of multicentric Castleman disease (MCD), targeting this pathway has been one of the primary focuses of treatment. Tocilizumab (TCZ) is a monoclonal antibody targeting the IL-6 receptor. In the three cases documenting its use in TAFRO syndrome, TCZ was combined with steroids resulting in improvement of symptoms and decreased markers of inflammation [7, 9, 11].As with the other TAFRO cases, our patient’s course was rapidly progressive. However, the combination of steroids, etoposide, rituximab, and TCZ was very effective in arresting the disease and reversing its end organ damage. This combination of therapy may be a novel effective approach in the management of this disorder and warrants consideration for this rare disease in future patients, especially if new guidelines are being investigated to treat TAFRO based upon disease severity [5].


## 3.1. Viral Etiology for TAFRO Syndrome

The patient described in this case report was positive for EBV DNA sequences by PCR in both the biopsied lymph nodes and bone marrow sample and had a subset of positive lymphoid cells by EBER on in situ hybridization. To date, no known virus has been associated with TAFRO syndrome; however, certain viruses are associated with MCD, namely, HHV-8 in HIV positive patients and EBV. This is a novel finding and may be a contributor to the etiology of TAFRO syndrome. In addition, the lymph node findings with a marked discrepancy between the low proliferation rate noted in atrophic germinal centers depleted of B lymphoid cells and the morphologically mature-appearing plasma cell proliferation noted in the interfollicular regions, associated with a high proliferation rate, may underline an extreme immune response leading to exhaustion of the germinal center cells and differentiation toward plasma cells secondary to strong antigenic stimulation. Furthermore, the reversed CD4-to-CD8 T-cell ratio identified in the peripheral blood and ascitic fluid in our patient, which initially raised in the differential diagnosis a possible HIV infection, has been noted in patients with other acute viral infections such as EBV and CMV, supporting also the possibility of an EBV driven TAFRO syndrome [6].


## 3.2. Treatment

Due to the rarity of TAFRO syndrome there are no guidelines to treatment. Also, the ambiguity of presenting symptoms and findings complicates treatment directions. In review of previous TAFRO cases, using PubMed search terms for confirmed TAFRO diagnosis, patterns of treatment can be seen (Table1). Most cases involved treatment with steroids [7–13]. Unfortunately, symptomatic improvement gained from steroids alone has usually been reported as short-lived [2, 14].Table 1
Summary of the most common treatment regimens for those diagnosed with TAFRO syndrome.
Case report
Age (years)/gender
TAFRO-directed treatment
General outcome
1st line
2nd line
3rd line
Simons, 2016
22/M
TCZ
TCZ, rituximab, etoposide
Clinical improvement
Tedesco, 2015
21/F
Prednisone, TCZ
Rituximab, cyclophosphamide, vincristine, prednisone
Clinical improvement
Takai et al., 2010 [17]
39/M
Methylprednisolone, prednisolone
Clinical improvement
38/M
Prednisolone
Clinical improvement
Koduri et al., 2014 [15]
14/M
CHOP× 6
Thalidomide
Persistent lymphadenopathy, no edema or ascites
Kubokawa et al., 2014 [7]
15/M
Methylprednisolone
TCZ, IVIG
Cytokine levels decreased by 4 months
Ozawa et al., 2014 [10]
48/F
IVIG
Steroids
Rituximab
Resolution of thrombocytopenia and marrow fibrosis
Kawabata et al., 2013 [9]
47/F
Prednisone
TCZ, steroids
Improvement of IL-6 levels, no signs of disease recurrence
Inoue et al., 2013 [8]
49/F
Dexamethasone
Cyclosporine A
Resolution of thrombocytopenia and marrow fibrosis
Iwaki et al., 2013 [11]
43/F
Methylprednisolone
Rituximab, TCZ, steroids
Improvement of thrombocytopenia and anasarca
Masaki et al., 2013 [12]
57/F
Methylprednisolone
CHOEP
Died from septic shock
73/M
Prednisolone
Died from multiorgan failure
Takai et al., 2010 [17]
47/F
CHOP, prednisolone
Prednisolone (maintenance)
Clinical improvement
56/M
Methylprednisolone, IVIG
Cyclosporin A
Improved platelet count, edema, ascites
49/M
Methylprednisolone, IVIG
Died from multiorgan failure
53/F
Prednisolone
Prednisolone, cyclophosphamide
Clinical improvement
56/F
Methylprednisolone, cyclosporin A
Clinical improvement
Abdo et al., 2014 [18]
81/M
Prednisone
Clinical improvementIn several cases, immunotherapeutic agents were utilized. Rituximab, a monoclonal antibody directed against the CD20 antigen on B-lymphocytes, has shown durable responses in HIV positive MCD [14]. Two cases of TAFRO syndrome reported clinical improvement with rituximab [8, 10]. In one case, it was used as monotherapy and, in the other, rituximab was used in combination with methylprednisolone and tocilizumab. Cyclosporin A was documented to have resulted in complete remission in four cases [8, 13].Ideally chemotherapy would provide a means to eliminate large portions of hypercytokine-secreting cells; however, its use without immunosuppressive therapy has not had lasting and meaningful results [12, 15]. Only one patient in the literature [16] had been treated with regimen consistent of rituximab, tocilizumab, cyclophosphamide, and etoposide.Since elevations in IL-6 have been thought to contribute to the pathophysiology of multicentric Castleman disease (MCD), targeting this pathway has been one of the primary focuses of treatment. Tocilizumab (TCZ) is a monoclonal antibody targeting the IL-6 receptor. In the three cases documenting its use in TAFRO syndrome, TCZ was combined with steroids resulting in improvement of symptoms and decreased markers of inflammation [7, 9, 11].As with the other TAFRO cases, our patient’s course was rapidly progressive. However, the combination of steroids, etoposide, rituximab, and TCZ was very effective in arresting the disease and reversing its end organ damage. This combination of therapy may be a novel effective approach in the management of this disorder and warrants consideration for this rare disease in future patients, especially if new guidelines are being investigated to treat TAFRO based upon disease severity [5].


## 4. Conclusion

TAFRO syndrome can be a rare, aggressive life-threatening disorder associated with multicentric Castleman disease which has only recently been described. The cases reported thus far have been almost exclusively in the Japanese literature. We present a case of a young American male to add to the body of literature of this unusual disorder and propose a possible viral cause and successful treatment regimen for those who have evidence of multiorgan failure.



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