# The Contribution of the Airway Epithelial Cell to Host Defense

Authors: Frauke Stanke
Journal: Mediators of Inflammation
 (2015)

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## Abstract

In the context of cystic fibrosis, the epithelial cell has been characterized in terms of its ion transport capabilities. The ability of an epithelial cell to initiate CFTR-mediated chloride and bicarbonate transport has been recognized early as a means to regulate the thickness of the epithelial lining fluid and recently as a means to regulate the pH, thereby determining critically whether or not host defense proteins such as mucins are able to fold appropriately. This review describes how the epithelial cell senses the presence of pathogens and inflammatory conditions, which, in turn, facilitates the activation of CFTR and thus directly promotes pathogens clearance and innate immune defense on the surface of the epithelial cell. This paper summarizes functional data that describes the effect of cytokines, chemokines, infectious agents, and inflammatory conditions on the ion transport properties of the epithelial cell and relates these key properties to the molecular pathology of cystic fibrosis. Recent findings on the role of cystic fibrosis modifier genes that underscore the role of the epithelial ion transport in host defense and inflammation are discussed.


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## Body

## 1. Introduction into the Defense Repertoire of the Epithelial Cell

The chloride and bicarbonate transporter CFTR, in healthy individuals as well as in F508del homozygous CF patients, is expressed on the apical surface of epithelial cells [1]. CFTR colocalizes with other ion channels such as the amiloride-sensitive epithelial sodium channel [2, 3]. The amount of liquid that covers the apical side of the epithelium is tightly regulated as the net ion transport through the apical membrane, driven by epithelial sodium and chloride channels, paralleled by water transport [3]. In turn, the amount and composition of the epithelial lining fluid determine the efficacy of mucociliary clearance [4], a mechanism by which the epithelium can detoxify pathogens and pollutants [4]. Host defense is mediated by resident macrophages that are localized on the epithelial surface [4] as well as by antimicrobial proteins that are secreted into the epithelial lining fluid [4]. The efficacy of mucociliary clearance depends on the beating of the epithelial cell’s cilia [4] and on the viscoelastic properties of the fluid that covers the epithelium [4, 5]. Thereby, CFTR directly influences the extent to which the fluid on the airway’s surface can be moved: firstly, chloride, and, in consequence, water, secreted via CFTR at the apical side of epithelial cells. Secondly, CFTR secretes bicarbonate, whereby the regulation of the pH determines whether secreted components such as mucins can unfold properly [6].Apart from ion channels, the apical membrane of epithelial cells is equipped with a variety of receptors that sense the presence of pathogens or the inflammatory state: toll-like receptors that directly interact with bacterial or viral components [7, 8] as well as receptors for macrophage-derived cytokines such as TNFα [9] and IFNγ [10] are expressed by epithelial cells [7, 8, 11, 12]. Hence, the epithelial cell is well equipped to detect the presence of pathogens as well as the activity of macrophages that reside in the epithelial surface fluid. In other words, the airway epithelial cell is in a unique position to recognize the need for host defense as well as providing it via an activation of the chloride and bicarbonate channel CFTR.


## 2. Cytokines Alter the Ion Conductance Capabilities of Airway Epithelial Cells

In order to recognize that cytokines can alter the ion secretion properties of epithelial cells, two fields that are traditionally not well linked, that is, experimental immunology and electrophysiology, need to interact. Fortunately, several experiments wherein airway epithelial cells have been exposed to cytokines and the expression or function of ion channels such as CFTR, the amiloride-sensitive epithelial sodium channel ENaC, and calcium-activated chloride channels is monitored by comparative RT-PCR, western blot, or electrophysiology have been provided since the turn of the century ([15–25]; Table 1). Roughly summarized, the uptake of sodium by airway epithelial cells through ENaC is inhibited by TGFβ [15, 25], IL13 [16, 22], IL1β [18], TNFα [19], IL4 [16], and IFNγ [17]. In contrast, the secretion of chloride and/or bicarbonate via CFTR is increased by IL17 [21], IL13 [16, 20], IL4 [16], and TNFα [17] and via the chloride transporter SLC26A9 by IL13 [23]. In conclusion, the data generated by independent researchers paints a highly coherent picture of the cross-talk between immunologically relevant cells and airway epithelial cells; cytokines, being released by immunologically active cells, will be interpreted by the epithelial cell as a signal to increase the epithelial surface fluid, thereby promoting mucociliary clearance and decreasing the amount of pathogens and inflammatory substances within the lung.Table 1
Effect of cytokines on ion transport in airway epithelial cells.
Model system
Cytokine
Ion channel
Techniques
Principle findings
Reference
EPub
Rabbit
TGFβ
ENaC
22Na+ efflux, electrophysiology, flow cytometry, surface biotinylation, and RT-PCR
TGFβ decreases ENaC-mediated sodium and fluid uptake
[25]
2014
Air liquid epithelial cell culture with human CFBE41o− and transfected cells
IL6, IL8, and CXCL1/2
CFTR, TMEM16A
Electrophysiology, immunocytochemistry, human cytokine antibody array, and cell-surface ELISA
CFTR and TMEM16A decrease secretion of IL6, IL8, and CXCL1/2
[24]
2012
Slc26a9-deficient mice
IL13
Slc26a9
Electrophysiology
IL13 increases Slc26a9-mediated Cl− secretion
[23]
2012
BALB/c mice
IL13
ENaC
Electrophysiology, RT-PCR
IL13 decreases ENaC expression
[22]
2010
Human bronchial epithelial cells
IL17A
Bicarbonate transport
Electrophysiology, measurement of surface and intracellular pH
IL17A increasesHC
O
3
- secretion
[21]
2009
Human nasal epithelial cells
IL13
CFTR
Immunocytochemistry, western blot
IL13 increases CFTR expression
[20]
2007
Primary culture of rat alveolar epithelial type II cells, primary culture of  human alveolar epithelial type II cells
Il1β
ENaC
22Na+ efflux, electrophysiology, western blot, and RT-PCR
IL1β decreases ENaC expression
[18]
2005
Rat alveolar epithelial cells
TNFα
ENaC
Electrophysiology, northern blot, and western blot
TNFα decreases ENaC expression
[19]
2004
Primary culture of rat alveolar epithelial type II cells, primary culture of  human alveolar epithelial type II cells
TGFβ
ENaC
22Na+ efflux, electrophysiology, western blot, and RT-PCR
TGFβ decreases ENaC expression
[15]
2003
Primary culture of human bronchial epithelial cells
IL4, IL13
CFTR, ENaC
Electrophysiology, western blot
IL4 and IL13 increase CFTR expressionIL4 and IL13 decrease ENaC expression
[16]
2002
Primary culture of human bronchial epithelial cells
IFNγ, TNFα
ENaC, CFTR
Electrophysiology, western blot, and transepithelial fluid transport
IFNγ decreases CFTR expressionIFNγ decreases ENaC-mediated sodium transportTNFα increases CFTR expression
[17]
2000The clinical importance of these findings is underlined (a) by the susceptibility of CFTR-deficient individuals to nosocomial pathogens, as observed in cystic fibrosis, (b) by the susceptibility of ENaC-deficient patients who suffer from pseudohypoaldosteronism type I [26] toP. aeruginosa [27, 28], and (c) by the elevated susceptibility of patients with CF-like disease carrying partially dysfunctional CFTR and/or ENaC gene variants to respiratory disease [29]. Furthermore, the impaired regulation of lung fluid balance by the cytokine TGFβ has now been recognized as a direct cause for acute respiratory distress syndrome [30].


## 3. Cystic Fibrosis Modifying Genes That Determine Immunology and Inflammation Alter the CFTR-Mediated Basic Defect

Modifier genes of cystic fibrosis disease severity have now been studied for a decade [31, 32]. Many studies are candidate genes based; that is, the investigators rely on a hypothesis of which of the 22.000 protein-coding human genes [33] is likely to influence CF disease. Several researchers have selected genes encoding for cytokines such asTNFA,IL1B, andTGFB1 as candidate genes because of their known role in infection, immunology, and inflammation [13, 34–38]. Among these immunologically relevant candidate genes,IL1B has been replicated in two truly independent studies [35, 39], albeit the molecular variant has not been mapped by the base yet. The cytokine receptorsTNFR1 andIFNGR1 have been studied as modifier genes inEuropean CF Twin and Sibling Study [13, 39, 40]. Until now, the CF basic defect that can be assessed by nasal potential difference measurement in vivo has only been used by theEuropean CF Twin and Sibling Study for an association study. Strikingly, the TNFα receptor 1 geneTNFR1 was observed as a modifier of CF disease severity as well as of CFTR-mediated residual chloride secretion in the nasal epithelium, whereby the risk and the benign allele were identified consistently for both traits ([13, 40], Figure 1). Furthermore, several immunologically relevant genes were identified as modifiers of the CFTR-mediated basic defect ([13], Table 2). This observation parallels the aforementioned observed capabilities of cytokines to activate fluid secretion by airway epithelial cells in order to promote clearance: as functional experiments using human airway epithelial cell lines, primary airway epithelial cells, and animal models have demonstrated that cytokines can alter ion and fluid transport in the respiratory epithelium effectively [15–25], it must be expected that genetic variants in cytokines and their receptors show genetic association with the manifestation of the basic defect among cystic fibrosis patients [13].Table 2
Immunologically relevant genes and transcription factors that were identified as modifiers of the CFTR-mediated basic defect among F508del-CFTR homozygous CF patients.
Functional category
Gene
Association observed with
Reference
Ligands
CD14
CFTR activity/intestinal tissue [C]Non-CFTR mediated residual chloride secretion/intestinal tissue [D]
[13]
TNFA
CFTR activity/nasal epithelium [B]
[13]
IL1B
CFTR activity/intestinal tissue [C]
[13, 39]
TGFB1
Non-CFTR mediated residual chloride secretion/intestinal tissue [D]
[13]
IL8
CFTR activity/nasal epithelium [B]
[13]
Membrane-bound receptors
TLR4
ENaC activity/nasal epithelium [A]CFTR activity/nasal epithelium [B]CFTR activity/intestinal tissue [C]
[13]
TNFR1
ENaC activity/nasal epithelium [A]CFTR activity/nasal epithelium [B]CFTR activity/intestinal tissue [C]Non-CFTR mediated residual chloride secretion/intestinal tissue [D]
[13]
CD95
ENaC activity/nasal epithelium [A]CFTR activity/nasal epithelium [B]CFTR activity/intestinal tissue [C]Non-CFTR mediated residual chloride secretion/intestinal tissue [D]
[13]
IFNGR1
CFTR activity/nasal epithelium [B]CFTR activity/intestinal tissue [C]
[13, 39]
TLR5
CFTR activity/nasal epithelium [B]Non-CFTR mediated residual chloride secretion/intestinal tissue [D]
[13]
TLR9
CFTR activity/intestinal tissue [C]Non-CFTR mediated residual chloride secretion/intestinal tissue [D]
[13]
Transcription factors
STAT3
CFTR activity/intestinal tissue [C]
[13, 39]
EHF
ENaC activity/nasal epithelium [A]CFTR activity/intestinal tissue [C]
[45]
[A]: the association was observed in a case-reference study comparing F508del-CFTR homozygotes with high versus low change of the potential upon superfusion of the nasal epithelium with amiloride as assessed by nasal potential difference measurement. Please see [13] for details.
[B]: the association was observed in a case-reference study comparing F508del-CFTR homozygotes with presence versus absence of a change of the potential upon superfusion of the nasal epithelium with chloride-free solution and isoproterenol as assessed by nasal potential difference measurement. Please see [13] for details.
[C]: the association was observed in a case-reference study comparing F508del-CFTR homozygotes with presence versus absence of DIDS-insensitive residual chloride secretion as assessed by intestinal current measurement of rectal suction biopsies. Please see [13] for details.
[D]: the association was observed in a case-reference study comparing F508del-CFTR homozygotes with presence versus absence of DIDS-sensitive residual chloride secretion of the intestinal tissue as assessed by intestinal current measurement of rectal suction biopsies. Please see [13] for details.Figure 1
Association ofTNFR1 variants with CF disease severity and manifestation of CFTR-mediated residual chloride secretion in respiratory tissue among F508del-CFTR homozygous CF patients. (a) Definition ofTNFR1 variants. Two contrasting haplotypes, designated haplotype 1 and haplotype 2 within this figure, were described by typing the five markers rs4149576, D12S889, rs4149577, rs4149580, and rs4149581 among 101 families with a total of 171 F508del-CFTR homozygous CF patients [13]. Haplotypes were reconstructed using the software FAMHAP [14]. Association was judged by case-reference association whereby cases and references were defined based on the disease severity of the siblings (b) or the manifestation of a change in potential upon superfusion of the nasal epithelium with chloride-free solution and isoproterenol (c). All P values reported within this figure were calculated by FAMHAP and are corrected for sibling dependence and testing of multiple markers [14]. Please note that the case and control subpopulations compared within (b) and (c) were defined independently and were nonoverlapping (for details, please see [13]). Please also note that haplotype 2, depicted in green within this figure, is overrepresented among sib pairs with mild CF disease and among patients who display CFTR-mediated residual chloride secretion by NPD. As a causal interpretation, this might reflect the crosstalk between the cytokine pathway and the ion secretory properties of the epithelium [15–25], indicating that the mildTNFR1 haplotype 2 is more susceptible to translating the action of the host defense modifier geneTNFR1 into CFTR-mediated residual function. Alternatively, as CFTR-mediated residual chloride secretion causes a mild disease phenotype, the observedTNFR1 association with the manifestation of the basic defect in NPD might reflect an overrepresentation of mild modifier alleles among patients with residual CFTR-mediated chloride secretion, which is equivalent to a replication study with confirmatory outcome.
(a)
(b)
(c)


## 4. Outlook

Functional data and genetic evidence indicate that the epithelial cell and the immune system interact to regulate ion and fluid secretion. It remains to be clarified by which molecular mechanism this is accomplished within the epithelial cell. Likely, part of the effect of cytokines on ion transport will be mediated by the signal transduction cascade that is set into motion by the contact between the soluble ligand and the membrane-bound receptor. While a target of such regulatory networks might be the CFTR gene itself, it is equally plausible that the configuration of the epithelial cell is altered to promote more efficient trafficking of the CFTR ion channel, known for its short half-life [41] and its prolonged residence in a subapical compartment [42–44], to the apical membrane. So far, an association with the CF basic defect has been described for genes encoding the two transcription factorsSTAT3 [39] and the epithelial-specific transcription factorEHF [45], the latter being a positional candidate that has been selected for replication based on a genome-wide study undertaken to identify modifiers of CF lung disease severity [46]. However, in order to effectively select therapeutic targets in the future, the central molecular pathways that are used by host defense modifier genes which have an impact on CFTR-mediated residual function or ENaC activity in epithelial cells need to be identified. Ultimately, a drug that interferes with the activity of key regulatory elements—such as regulatory microRNAs and transcription factors—which translate the action of host defense modifier genes into CFTR-mediated residual function or ENaC activity will be an attractive instrument to counterbalance the susceptibility of CF patients to infection and inflammation.



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