# Uncoupling Protein 2 and Peroxisome Proliferator-Activated Receptorγ Gene Polymorphisms in Association with Diabetes Susceptibility in Chinese Han Population with Variant Glucose Tolerance

Authors: Meicen Zhou; Shuli He; Fan Ping; Wei Li; Lixin Zhu; Xiangli Cui; Linbo Feng; Xuefeng Zhao; Huabing Zhang; Yuxiu Li; Qi Sun
Journal: International Journal of Endocrinology
 (2018)

---

## Abstract

Objective. To investigate the association of polymorphisms in uncoupling protein 2 (UCP2) and peroxisome proliferator-activated receptor (PPARγ) with glucolipid metabolism in Chinese Han population. Methods. Five hundred eighty-nine subjects were divided into normal glucose tolerance (NGT) group (n=198) and abnormal glucose tolerance group (n=358). HbA1c, blood lipid profile, plasma glucose, and insulin were determined. Insulin sensitivity (HOMA-IR and Matsuda index (ISIM)) and insulin secretion indexes (HOMA-β, early and total phase disposition index) were evaluated. Eight potential functional SNPs in UCP2 and 7 in PPARγ were selected. SNPs were genotyped on Sequenom MassARRAY platform. Results. The GG genotype of rs2920502 in PPARγ was associated with decreased risk of impaired glucose tolerance (G allele: OR: 0.818, 95%CI: 0.526–0.969, P=0.042; GG: OR: 0.715, 95%CI: 0.527–0.97, P=0.031). The TT genotype of rs3856806 in PPARγ was associated with increased risk of impaired glucose tolerance (T allele: OR: 1.46, 95%CI: 1.055–2.017, P=0.022; TT: OR: 1.58, 95%CI: 1.104–2.761, P=0.032). The GG genotype of rs2920502 in PPARγ had better blood glucose and increased insulin secretion and had lower HOMA-IR than GC/CC genotypes. Conclusion. It probably could prevent insulin resistance in early stage by classifying the genotype of rs649446 and rs7109266 in UCP2. The GG genotype of rs2920502 in PPARγ had a decreased risk for diabetes. The TT genotype of rs3856806 in PPARγ had an increased risk for diabetes.


---

## Body

## 1. Introduction

Uncoupling protein 2 (UCP2), which is widely expressed in human tissues and serves as an uncoupler of oxidative phosphorylation, is involved in the regulation of glucolipid metabolism and ATP production [1, 2]. The association of the polymorphisms in UCP2 with diabetes and obesity have been widely evaluated, most studies focused on Ala55Val (rs660339) in exon 4, 45 bp insertion/deletion in exon 8, and -866G/A (rs659336) in the promoter region [3, 4]. The polymorphisms in UCP2 regulate the expression of mRNA and protein, which have vital effects on islet β-cell function and insulin sensitivity [5, 6]. The -866AA genotype carriers have decreased glucose-stimulated insulin secretion and have increased risk of diabetes than those GG genotype carriers [7]. Although a variant allele of the Ala55Val polymorphism was reported to be associated with lower energy expenditure and the 45 bp insertion/deletion polymorphisms were found to be functional on mRNA expression, the association of Ala55Val (rs660339) in exon 4 with diabetes remain controversial [8–10].Peroxisome proliferator-activated receptor (PPARs) play pivotal roles in the control of the transcription of UCP2 [11, 12]. PPARs have three isoforms, including Pparα, Pparδ, and PPARγ. PPARγ is a regulator of lipid and glucose metabolism and therefore its synthetic ligands such as glitazone—the derivative of thiazolidinediones (e.g., troglitazone, rosiglitazone, and pioglitazone)—improve insulin and glucose parameters and increase whole body insulin sensitivity [13]. These PPARγ synthetic ligands could indirectly increase insulin-stimulated glucose uptake in adipocytes, skeletal muscle cells, and hepatocytes [13]. Our previous study found that the UCP2-deficient mice fed with a long-term high-fat diet had better insulin sensitivity, improved lipid metabolism, and upregulated expression of PPARγ in PPAR signaling pathway, which suggested the ameliorated lipid metabolism and insulin sensitivity in UCP2-deficient mice probably via PPARγ. It was most likely that among Ppar isoforms, PPARγ was the major regulator of UCP2 in high-fat diet [14]. One study based on Chinese Han population showed that functional SNPs of PPARγ were associated with MetS [15]. The relationship between potential functional SNPs and diabetes remains unknown.The inflammation pathway is involved in the pathophysiology of diabetes and obesity. Previous study showed that PPAR polymorphisms were independently associated with CRP levels in Chinese Han population; PPARs polymorphisms interact with overweight/obesity to set CRP levels [16]. In healthy children and adolescents, UCP2 -866G>A modified low-grade inflammatory state [17]. Whether UCP2 and PPARγ polymorphisms have an effect on inflammation state in diabetes remains unknown.In this study, we built a Chinese Han population cohort with variant glucose tolerance and aimed to further investigate the association of polymorphisms in the functional region of UCP2 and PPARγ with glucolipid metabolism.


## 2. Subjects and Methods

### 2.1. Subjects

All subjects were recruited from a type 2 diabetes project in a Beijing suburb in China between March 2014 and January 2015. Five hundred eighty-nine subjects without a history of diabetes underwent a 75 g OGTT. The 75 g OGTT was conducted after an overnight fast (>10 hours). Blood samples were collected at 0 minutes, 30 minutes, 60 minutes, and 120 minutes following the OGTT. The glucose tolerance status of each subject was classified based on the 1999 criteria of the WHO: a normal glucose tolerance (NGT), indicated by fasting plasma glucose (FPG) < 6.1 mmol/l and 2 h postprandial glucose (2 h PG) < 7.8 mmol/l; prediabetes, indicated by impaired fasting glucose (IFT): 6.1 mmol/l ≤ FPG < 7.0 mmol/l and 2 h PG < 7.8 mmol/l; impaired glucose tolerance (IGT), indicated by FPG < 6.1 mmol/l and 7.8 ≤ 2 h PG < 11.1 mmol/l; or IFT + IGT, with T2DM indicated by FPG ≥ 7.0 mmol/l or 2 h PG ≥ 11.1 mmol/l.The subjects who have a current history of cigarette smoking and alcohol drinking were excluded, and subjects with serious diseases such as heart disease, stroke, kidney disease, liver disease, and inflammatory disease were also excluded. Subjects who were on steroids or who were taking drugs interfering with lipid metabolism such as lipid-lowering agents, diuretics,β-blockers, and fish oil were excluded. On the basis of the 75 g OGTT results, subjects were divided into normal glucose tolerance (NGT) group (n=198) and abnormal glucose tolerance group (n=358). The study protocol was approved by the Ethics Committee of Peking Union Medical College Hospital. The subjects voluntarily signed informed consent forms.


### 2.2. Clinical Measurement

A standardized medical history and accurate physical examination were undertaken in all of the subjects before a 75 g OGTT was administered. Measurements of waist circumference (WC) (midway between the iliac crest and the costal margin) and hip circumference (HC) (at the level of the trochanters) were performed twice by the same observer, and the mean value was recorded. Weight and height were measured without shoes in light clothing, and body mass index (BMI) was calculated by dividing the body weight in kilograms by the square of the height in meters. Blood pressure measurements were obtained twice with a standard mercury sphygmomanometer with the subjects at rest, and the mean value was calculated.


### 2.3. Biochemical Measurements

Plasma glucose was measured by glucose oxidase assay. TC, TG, HDL-C, and LDL-C were determined using an automated analyzer. Serum insulin and C peptide were measured by chemiluminescent enzyme immunoassay. HbA1c analysis was performed by high-performance liquid chromatography (intra-assay CV < 3%, interassay CV < 10%).


### 2.4. Assessment of IR

Homeostatic model assessment of insulin resistance (HOMA-IR) was calculated to evaluate the IR [18].


### 2.5. Assessment ofβ-Cell Function

The homeostasis model assessment of insulin secretion (HOMA-β) was calculated as basal insulin release [18]. Early-phase insulin release was calculated as the total insulin area under the curve divided by the total glucose area under the curve during the first 30 min of the OGTT (InsAUC30/GluAUC30), which was shown to have a strong correlation with first-phase insulin secretion [19]. Insulin secretion relative to insulin sensitivity (ISIM: Matsuda insulin sensitivity index) was expressed as the disposition index (DI), calculated as early-phase DI30 = [InsAUC30/GluACU30] × ISIM, (ΔIns30/ΔGlu30)/HOMA-IR and total-phase DI120 = [InsAUC120/GluACU120] × ISIM. Another formula for assessing early-phase insulin release was (ΔIns30/ΔGlu30)/HOMA-IR.


### 2.6. Measurement of Tumor Necrosis Factor-α (TNF-α) and Interleukine-6 (IL-6)

Serums were from fasting blood samples. The levels of TNF-α and IL-6 were performed as per the manufacturer’s instructions (Cloud-Clone Corp., Houston, USA), and absorbance kinetics was measured through an ELISA reader.


### 2.7. SNP Selection, Genotyping, and Genotype Quality Control

Genomic DNA was extracted from peripheral blood samples using the QIAamp DNA blood mid kit (Qiagen, Hilden, Germany); purified DNA samples were diluted and quantified using a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific, Wilmington, DE, USA). We selected 8 potential functional SNPs of UCP2 and 7 potential functional SNPs of PPARγ, including promoter, exon, 5′ untranslated region and 3′ untranslated region based on the screening standards (the minor allele frequencies (MAF) are more than 20% in Han Chinese according to the HapMap Han Chinese in Beijing (CHB) group). Further, we reviewed the documents about the selected SNPs and forecasted their function according to NIH SNPinfo Web Server (http://snpinfo.niehs.nih.gov/) (Table 1). The MAFs of the selected SNPs in the study were listed in Table 2. All candidate SNPs were genotyped on Sequenom MassARRAY platform.Table 1
The selected functional SNPs of UCP2 and PPARγ.
Gene name
SNP number
Function
Minor allele frequency
Data sources
Relevant documents
Function forecast
UCP2
rs660339
Missense
0.422
HapMap
Investigation of variants in UCP2 in Chinese type 2 diabetes and diabetic retinopathy
Splicing (ESE or ESS)/nsSNP
rs659366
Promoter
0.442
HapMap
The common -866G/A polymorphism in the promoter region of the UCP-2 gene is associated with reduced risk of type 2 diabetes in Caucasians from Italy
TFBS
rs649446
Promoter
0.35
HapMap
No report
TFBS
rs586773
5′ near
0.476
1000 Genomes
No report
TFBS
rs34408426
5′ near
0.476
1000 Genomes
No report
TFBS
rs7109266
5′ near
0.349
HapMap
No report
TFBS
rs3019463
5′ near
0.476
1000 Genomes
No report
TFBS
rs591758
5′ near
0.422
HapMap
Genetic variants in the UCP2-UCP3 gene cluster and risk of diabetes in the Women’s Health Initiative Observational Study
TFBS
PPARγ
rs3856806
Cds-synon
0.233
HapMap
Gene-gene interactions among PPARα/δ/γ polymorphisms for hypertriglyceridemia in Chinese Han population
—
rs2920502
Promoter
0.244
HapMap
Genetic variants in peroxisome proliferator-activated receptor-γ and retinoid X receptor-α gene and type 2 diabetes risk: a case-control study of a Chinese Han population
TFBS
rs17029007
5′ UTR
0.102
1000 Genomes
No report
TFBS/splicing
rs73021485
Promoter
0.374
1000 Genomes
No report
—
rs73813168
5′ near
0.103
1000 Genomes
No report
—
rs2920503
5′ near
0.32
1000 Genomes
No report
—
rs79310821
5′ near
0.371
1000 Genomes
No report
TFBS: transcription factor binding site.Table 2
The MAFs of the selected SNPs in the study.
Chromosome
Gene name
SNP number
Alleles
Detection rate (%)
MAF in CHB
MAF in the study
11
UCP2
rs660339
A/G
99.8
0.42
0.47
rs659366
T/C
99.8
0.44
0.48
rs649446
T/C
99.8
0.35
0.34
rs586773
T/A
98.9
0.48
0.48
rs34408426
G/A
99.3
0.48
0.48
rs7109266
A/G
99.8
0.35
0.33
rs3019463
T/C
97.4
0.48
0.48
rs591758
C/G
99.8
0.42
0.48
3
PPARγ
rs2920503
A/G
97.7
0.32
0.31
rs73813168
G/A
98.5
0.10
0.12
rs79310821
A/G
99.2
0.37
0.35
rs73021485
T/G
99.7
0.37
0.35
rs2920502
G/C
99.3
0.24
0.31
rs17029007
A/G
98.9
0.10
0.13
rs3856806
T/C
99.5
0.23
0.20
MAF: minor allele frequency; CHB: Han Chinese in Beijing.


### 2.8. Statistical Analysis

Continuous variables were expressed as mean ± standard deviations (SD). Statistical significances for continuous variables were assessed using Student’st-test and for categorical variables using chi-square test. Hardy-Weinberg equilibrium tests were performed using Pearson’s chi-square for each SNP among control subjects. One-way ANOVA was used to compare different genotypes of every SNP site for continuous variables. All the statistical analyses were performed using SPSS 19.0 for windows and SAS 9.2 (SAS Institute) and a P value of <0.05 was considered statistically significant.


## 2.1. Subjects

All subjects were recruited from a type 2 diabetes project in a Beijing suburb in China between March 2014 and January 2015. Five hundred eighty-nine subjects without a history of diabetes underwent a 75 g OGTT. The 75 g OGTT was conducted after an overnight fast (>10 hours). Blood samples were collected at 0 minutes, 30 minutes, 60 minutes, and 120 minutes following the OGTT. The glucose tolerance status of each subject was classified based on the 1999 criteria of the WHO: a normal glucose tolerance (NGT), indicated by fasting plasma glucose (FPG) < 6.1 mmol/l and 2 h postprandial glucose (2 h PG) < 7.8 mmol/l; prediabetes, indicated by impaired fasting glucose (IFT): 6.1 mmol/l ≤ FPG < 7.0 mmol/l and 2 h PG < 7.8 mmol/l; impaired glucose tolerance (IGT), indicated by FPG < 6.1 mmol/l and 7.8 ≤ 2 h PG < 11.1 mmol/l; or IFT + IGT, with T2DM indicated by FPG ≥ 7.0 mmol/l or 2 h PG ≥ 11.1 mmol/l.The subjects who have a current history of cigarette smoking and alcohol drinking were excluded, and subjects with serious diseases such as heart disease, stroke, kidney disease, liver disease, and inflammatory disease were also excluded. Subjects who were on steroids or who were taking drugs interfering with lipid metabolism such as lipid-lowering agents, diuretics,β-blockers, and fish oil were excluded. On the basis of the 75 g OGTT results, subjects were divided into normal glucose tolerance (NGT) group (n=198) and abnormal glucose tolerance group (n=358). The study protocol was approved by the Ethics Committee of Peking Union Medical College Hospital. The subjects voluntarily signed informed consent forms.


## 2.2. Clinical Measurement

A standardized medical history and accurate physical examination were undertaken in all of the subjects before a 75 g OGTT was administered. Measurements of waist circumference (WC) (midway between the iliac crest and the costal margin) and hip circumference (HC) (at the level of the trochanters) were performed twice by the same observer, and the mean value was recorded. Weight and height were measured without shoes in light clothing, and body mass index (BMI) was calculated by dividing the body weight in kilograms by the square of the height in meters. Blood pressure measurements were obtained twice with a standard mercury sphygmomanometer with the subjects at rest, and the mean value was calculated.


## 2.3. Biochemical Measurements

Plasma glucose was measured by glucose oxidase assay. TC, TG, HDL-C, and LDL-C were determined using an automated analyzer. Serum insulin and C peptide were measured by chemiluminescent enzyme immunoassay. HbA1c analysis was performed by high-performance liquid chromatography (intra-assay CV < 3%, interassay CV < 10%).


## 2.4. Assessment of IR

Homeostatic model assessment of insulin resistance (HOMA-IR) was calculated to evaluate the IR [18].


## 2.5. Assessment ofβ-Cell Function

The homeostasis model assessment of insulin secretion (HOMA-β) was calculated as basal insulin release [18]. Early-phase insulin release was calculated as the total insulin area under the curve divided by the total glucose area under the curve during the first 30 min of the OGTT (InsAUC30/GluAUC30), which was shown to have a strong correlation with first-phase insulin secretion [19]. Insulin secretion relative to insulin sensitivity (ISIM: Matsuda insulin sensitivity index) was expressed as the disposition index (DI), calculated as early-phase DI30 = [InsAUC30/GluACU30] × ISIM, (ΔIns30/ΔGlu30)/HOMA-IR and total-phase DI120 = [InsAUC120/GluACU120] × ISIM. Another formula for assessing early-phase insulin release was (ΔIns30/ΔGlu30)/HOMA-IR.


## 2.6. Measurement of Tumor Necrosis Factor-α (TNF-α) and Interleukine-6 (IL-6)

Serums were from fasting blood samples. The levels of TNF-α and IL-6 were performed as per the manufacturer’s instructions (Cloud-Clone Corp., Houston, USA), and absorbance kinetics was measured through an ELISA reader.


## 2.7. SNP Selection, Genotyping, and Genotype Quality Control

Genomic DNA was extracted from peripheral blood samples using the QIAamp DNA blood mid kit (Qiagen, Hilden, Germany); purified DNA samples were diluted and quantified using a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific, Wilmington, DE, USA). We selected 8 potential functional SNPs of UCP2 and 7 potential functional SNPs of PPARγ, including promoter, exon, 5′ untranslated region and 3′ untranslated region based on the screening standards (the minor allele frequencies (MAF) are more than 20% in Han Chinese according to the HapMap Han Chinese in Beijing (CHB) group). Further, we reviewed the documents about the selected SNPs and forecasted their function according to NIH SNPinfo Web Server (http://snpinfo.niehs.nih.gov/) (Table 1). The MAFs of the selected SNPs in the study were listed in Table 2. All candidate SNPs were genotyped on Sequenom MassARRAY platform.Table 1
The selected functional SNPs of UCP2 and PPARγ.
Gene name
SNP number
Function
Minor allele frequency
Data sources
Relevant documents
Function forecast
UCP2
rs660339
Missense
0.422
HapMap
Investigation of variants in UCP2 in Chinese type 2 diabetes and diabetic retinopathy
Splicing (ESE or ESS)/nsSNP
rs659366
Promoter
0.442
HapMap
The common -866G/A polymorphism in the promoter region of the UCP-2 gene is associated with reduced risk of type 2 diabetes in Caucasians from Italy
TFBS
rs649446
Promoter
0.35
HapMap
No report
TFBS
rs586773
5′ near
0.476
1000 Genomes
No report
TFBS
rs34408426
5′ near
0.476
1000 Genomes
No report
TFBS
rs7109266
5′ near
0.349
HapMap
No report
TFBS
rs3019463
5′ near
0.476
1000 Genomes
No report
TFBS
rs591758
5′ near
0.422
HapMap
Genetic variants in the UCP2-UCP3 gene cluster and risk of diabetes in the Women’s Health Initiative Observational Study
TFBS
PPARγ
rs3856806
Cds-synon
0.233
HapMap
Gene-gene interactions among PPARα/δ/γ polymorphisms for hypertriglyceridemia in Chinese Han population
—
rs2920502
Promoter
0.244
HapMap
Genetic variants in peroxisome proliferator-activated receptor-γ and retinoid X receptor-α gene and type 2 diabetes risk: a case-control study of a Chinese Han population
TFBS
rs17029007
5′ UTR
0.102
1000 Genomes
No report
TFBS/splicing
rs73021485
Promoter
0.374
1000 Genomes
No report
—
rs73813168
5′ near
0.103
1000 Genomes
No report
—
rs2920503
5′ near
0.32
1000 Genomes
No report
—
rs79310821
5′ near
0.371
1000 Genomes
No report
TFBS: transcription factor binding site.Table 2
The MAFs of the selected SNPs in the study.
Chromosome
Gene name
SNP number
Alleles
Detection rate (%)
MAF in CHB
MAF in the study
11
UCP2
rs660339
A/G
99.8
0.42
0.47
rs659366
T/C
99.8
0.44
0.48
rs649446
T/C
99.8
0.35
0.34
rs586773
T/A
98.9
0.48
0.48
rs34408426
G/A
99.3
0.48
0.48
rs7109266
A/G
99.8
0.35
0.33
rs3019463
T/C
97.4
0.48
0.48
rs591758
C/G
99.8
0.42
0.48
3
PPARγ
rs2920503
A/G
97.7
0.32
0.31
rs73813168
G/A
98.5
0.10
0.12
rs79310821
A/G
99.2
0.37
0.35
rs73021485
T/G
99.7
0.37
0.35
rs2920502
G/C
99.3
0.24
0.31
rs17029007
A/G
98.9
0.10
0.13
rs3856806
T/C
99.5
0.23
0.20
MAF: minor allele frequency; CHB: Han Chinese in Beijing.


## 2.8. Statistical Analysis

Continuous variables were expressed as mean ± standard deviations (SD). Statistical significances for continuous variables were assessed using Student’st-test and for categorical variables using chi-square test. Hardy-Weinberg equilibrium tests were performed using Pearson’s chi-square for each SNP among control subjects. One-way ANOVA was used to compare different genotypes of every SNP site for continuous variables. All the statistical analyses were performed using SPSS 19.0 for windows and SAS 9.2 (SAS Institute) and a P value of <0.05 was considered statistically significant.


## 3. Results

### 3.1. Allele Frequency Analysis

All loci conformed to Hardy-Weinberg equilibrium as shown in Supplementary Table1. There was no significant difference in allele frequency of each SNP in UCP2 between prediabetes/diabetes group and normal glucose tolerance group (Table 3). In PPARγ, the G allele in rs2920502 decreased the risk of diabetes (OR: 0.818, 95%CI: 0.526–0.969, P=0.042), the T allele in rs3856806 increased the risk of diabetes (OR: 1.46, 95%CI: 1.055–2.017, P=0.022) (Table 3). In UCP2, there was no significant difference between alleles in each SNP.Table 3
Allele frequency analysis between prediabetes/diabetes group and normal blood glucose group.
Gene name
SNP number
Allele
Prediabetes/diabetes group
Normal blood glucose group
Odd ratio (OR)
95% confidence interval (CI)
P value
(N)
(N)
Low
High
UCP2
rs660339
A
346
187
0.997
0.775
1.281
0.9778
G
388
209
rs659366
T
351
192
0.973
0.758
1.248
0.828
C
383
204
rs649446
T
244
136
0.949
0.728
1.238
0.700
C
490
260
rs586773
T
351
192
0.959
0.750
1.227
0.741
A
381
200
rs34408426
G
350
193
0.959
0.750
1.227
0.738
A
380
201
rs7109266
A
240
135
0.935
0.715
1.222
0.624
G
494
261
rs3019463
T
343
188
0.978
0.763
1.253
0.861
C
373
200
rs591758
C
352
194
0.959
0.750
1.227
0.738
G
382
202
PPARγ
rs2920503
A
213
126
0.918
0.708
1.191
0.519
G
499
270
rs73813168
G
80
58
0.693
0.474
1.012
0.057
A
646
334
rs79310821
A
265
126
1.2
0.924
1.560
0.172
G
467
266
rs73021485
T
271
127
1.241
0.957
1.609
0.104
G
463
269
rs2920502
G
214
132
0.818
0.526
0.969
0.042∗
C
516
262
rs17029007
A
81
60
0.699
0.486
1.005
0.053
G
645
336
rs3856806
T
163
66
1.460
1.055
2.017
0.022∗
C
567
330
∗P<0.05.


### 3.2. Genotype Analysis

The association of SNPs with prediabetes/diabetes was assessed by crosstab test and logistic regression after adjustment for age and sex. In PPARγ, the frequency of GG genotype in rs2920502 was significantly lower in prediabetes/diabetes subjects (6.85%) than in the normal glucose tolerance subjects (12.69%); logistic regression analysis revealed that subjects with GG genotype of rs2920502 in PPARγ had less risk for prediabetes/diabetes compared to CC genotype (odd ratio (OR): 0.715; 95% confidence interval (CI): 0.527–0.97, P=0.031). The frequency of TT genotype in rs3856806 was significantly higher in prediabetes/diabetes subjects than in the normal glucose tolerance subjects; logistic regression analysis showed that subjects with TT genotype of rs3856806 in PPARγ had higher risk for diabetes compared to CC (OR: 1.58, 95%CI: 1.104–2.761, P=0.032). Furthermore, we, respectively, performed a logistic regression analysis under a recessive inheritance model (GG versus GC + CC) in rs2920502 and a dominant inheritance model (TT + TC/CC) in rs3856806; the regression showed that the odd ratio for GG versus GC + CC in rs2920502 was 0.506 (95%CI: 0.282–0.906, P=0.022) and the odd ratio for TT + TC/CC in rs3856806 was 1.479 (95%CI: 1.026–2.133, P=0.036). These were in accordance with the allele frequency analysis, which implied that G allele carriers in rs2920502 were less susceptible to develop diabetes and T allele carriers in rs3856806 were more susceptible to develop diabetes. No significant difference was found at other loci in PPARγ (Table 4). There was no significant difference in the genotype of each SNPs in UCP2 (Table 4).Table 4
Genotype analysis between prediabetes/diabetes group and normal blood glucose group.
Gene name
SNP number
Genotype
Prediabetes/diabetes group
Normal blood glucose group
Odd ratio (OR)
95% confidence interval (CI)
P value
Low
High
UCP2
rs660339
AA
77
42
0.996
0.774
1.28
0.9781
AG
192
103
GG
98
53
rs659366
TT
80
45
0.972
0.757
1.247
0.968
TC
191
102
CC
96
51
rs649446
TT
38
19
1.003
0.739
1.362
0.699
TC
168
98
CC
161
81
rs586773
TT
83
47
0.959
0.749
1.226
0.9377
TA
185
98
AA
98
51
rs34408426
GG
83
47
0.959
0.749
1.226
0.9444
GA
184
99
AA
98
51
rs7109266
AA
36
18
0.997
0.729
1.363
0.631
AG
168
99
GG
163
81
rs3019463
TT
82
45
0.979
0.763
1.255
0.9793
TC
179
98
CC
97
51
rs591758
CC
83
47
0.959
0.749
1.226
0.9443
CG
186
100
GG
98
51
PPARγ
rs2920503
AA
34
23
0.891
0.665
1.193
0.438
AG
145
80
GG
177
95
rs73813168
GG
2
2
0.701
0.262
1.877
0.480
AA
285
140
AG
76
54
rs79310821
AA
44
23
1.108
0.834
1.471
0.480
AG
177
80
GG
145
93
rs73021485
TT
47
23
1.159
0.875
1.535
0.304
GG
143
94
GT
177
81
rs2920502
GG
25
25
0.715
0.527
0.97
0.031∗
CC
176
90
CG
164
82
rs17029007
AA
5
3
0.905
0.439
1.864
0.786
AG
71
54
GG
287
141
rs3856806
TT
16
3
1.58
1.104
2.761
0.032∗
CC
218
136
CT
131
58
∗P<0.05.


### 3.3. Haplotype Analysis

There was a linkage disequilibrium in PPARγ and UCP2, respectively. The haplotype frequency distribution of each gene between prediabetes/diabetes and normal glucose tolerance was summarized in Table 5; however, haplotype frequency was not significantly different between prediabetes/diabetes and control.Table 5
The haplotype frequency distribution between prediabetes/diabetes and normal glucose tolerance.
Gene name
Haplotype
Prediabetes/diabetes, normal glucose tolerance frequency
χ2
P value
UCP2
GCCAAGCG
0.511, 0.513
0.003
0.953
ATTTGATC
0.318, 0.329
0.133
0.715
ATCTGGTC
0.146, 0.143
0.021
0.884
PPARγ
CAATCG
0.362, 0.316
2.473
0.1158
TAGGCG
0.307, 0.318
0.149
0.6992
CAGGGG
0.182, 0.184
0.008
0.9304
CGGGGA
0.109, 0.146
3.268
0.0706
CAGGCG
0.029, 0.025
0.147
0.7015


### 3.4. Association of Genotype with Demographic Characteristics

In UCP2, the waist-to-hip ratio in subjects with AA genotype of rs7109266 were higher than that in subjects with GG or GA genotype, but age, BMI, and blood pressure were not different among genotypes of other SNPs (Table6). Age, BMI, blood pressure, and waist-to-hip ratio were not different among genotypes of selected SNPs in PPARγ (Table 7).Table 6
Association of genotype and demographic characteristics in UCP2.
Genotype
Age (year)
BMI (kg/m2)
Waist-to-hip ratio
Systolic blood pressure (mmHg)
Diastolic blood pressure (mmHg)
rs660339
GG
52.87 ± 1.03
25.62 ± 0.29
0.94 ± 0.00
129.51 ± 1.61
75.28 ± 0.78
AA
54.22 ± 1.00
25.94 ± 0.35
0.95 ± 0.02
128.74 ± 1.78
76.42 ± 0.96
GA
54.00 ± 0.66
26.07 ± 0.23
0.94 ± 0.00
126.78 ± 1.03
76.62 ± 0.59
P value
0.557
0.51
0.492
0.3
0.405
rs659366
CC
53.26 ± 1.03
25.55 ± 0.28
0.94 ± 0.00
129.69 ± 1.65
75.39 ± 0.79
TT
54.48 ± 1.00
25.91 ± 0.34
0.95 ± 0.02
128.60 ± 1.76
76.15 ± 0.93
TC
53.68 ± 0.67
26.11 ± 0.23
0.94 ± 0.00
126.75 ± 1.02
76.67 ± 0.59
P value
0.683
0.353
0.503
0.269
0.452
rs649446
CC
52.47 ± 0.78
25.63 ± 0.23
0.94 ± 0.00
128.65 ± 1.18
75.85 ± 0.64
TT
56.44 ± 1.51
26.71 ± 0.48
0.97 ± 0.04
128.98 ± 2.59
74.18 ± 1.41
TC
54.29 ± 0.68
26.02 ± 0.25
0.94 ± 0.00
126.87 ± 1.15
76.91 ± 0.61
P value
0.058
0.132
0.055
0.5
0.138
rs7109266
GG
52.61 ± 0.78
25.64 ± 0.23
0.93 ± 0.00
128.65 ± 1.17
75.78 ± 0.64
AA
56.28 ± 1.57
26.65 ± 0.50
0.98 ± 0.04
129.26 ± 2.70
74.69 ± 1.44
GA
54.24 ± 0.68
26.03 ± 0.25
0.94 ± 0.00
126.82 ± 1.14
76.84 ± 0.61
P value
0.068
0.177
0.017∗
0.456
0.254
rs591758
GG
53.24 ± 1.03
25.59 ± 0.28
0.94 ± 0.00
129.77 ± 1.63
75.56 ± 0.78
CC
54.71 ± 0.99
25.95 ± 0.34
0.95 ± 0.02
128.62 ± 1.76
76.05 ± 0.90
CG
53.57 ± 0.67
26.08 ± 0.24
0.94 ± 0.00
126.64 ± 1.03
76.64 ± 0.60
P value
0.539
0.45
0.43
0.224
0.558
rs586773
AA
53.24 ± 1.03
25.59 ± 0.28
0.94 ± 0.00
129.77 ± 1.63
75.56 ± 0.78
TT
54.71 ± 0.99
25.95 ± 0.34
0.95 ± 0.02
128.62 ± 1.76
76.05 ± 0.90
AT
53.64 ± 0.68
26.07 ± 0.24
0.94 ± 0.00
126.67 ± 1.03
76.66 ± 0.61
P value
0.553
0.459
0.436
0.235
0.548
rs34408426
AA
53.24 ± 1.03
25.59 ± 0.28
0.94 ± 0.00
129.77 ± 1.63
75.56 ± 0.78
GG
54.71 ± 0.99
25.95 ± 0.34
0.95 ± 0.02
128.62 ± 1.76
76.05 ± 0.90
AG
53.64 ± 0.67
26.06 ± 0.24
0.94 ± 0.00
126.77 ± 1.03
76.74 ± 0.60
P value
0.552
0.478
0.43
0.26
0.495
rs3019463
CC
53.24 ± 1.04
25.60 ± 0.28
0.94 ± 0.00
129.84 ± 1.64
75.53 ± 0.79
TT
54.71 ± 1.01
25.98 ± 0.34
0.95 ± 0.02
128.83 ± 1.79
76.04 ± 0.92
TC
53.58 ± 0.68
26.03 ± 0.24
0.94 ± 0.00
126.29 ± 1.03
76.38 ± 0.61
P value
0.551
0.519
0.429
0.138
0.709
∗P<0.05.Table 7
Association of genotype and demographic characteristics in PPARγ.
Genotype
Age (year)
BMI (kg/m2)
Waist-to-hip ratio
Systolic blood pressure (mmHg)
Diastolic blood pressure (mmHg)
rs2920503
CC
53.99 ± 0.71
26.09 ± 0.22
0.94 ± 0.01
128.06 ± 1.10
76.26 ± 0.57
CT
54.25 ± 0.74
25.69 ± 0.27
0.94 ± 0.00
128.02 ± 1.29
75.99 ± 0.71
TT
50.03 ± 1.54
25.78 ± 0.47
0.94 ± 0.00
126.33 ± 2.44
75.91 ± 1.45
P value
0.430
0.502
0.583
0.806
0.946
rs73813168
AA
53.79 ± 0.57
25.92 ± 0.19
0.94 ± 0.01
128.15 ± 0.87
76.04 ± 0.48
GA
53.41 ± 1.00
25.89 ± 0.32
0.94 ± 0.01
127.27 ± 1.82
77.02 ± 0.93
GG
52.25 ± 6.13
28.91 ± 1.87
0.94 ± 0.01
124.75 ± 15.30
69.00 ± 4.65
P value
0.92
0.29
0.995
0.845
0.215
rs79310821
GA
54.33 ± 0.67
25.73 ± 0.23
0.94 ± 0.00
127.97 ± 1.06
76.23 ± 0.61
GG
52.11 ± 0.77
26.19 ± 0.25
0.94 ± 0.00
127.57 ± 1.30
76.31 ± 0.69
AA
53.66 ± 1.55
25.77 ± 0.52
0.93 ± 0.01
128.56 ± 2.43
75.95 ± 1.11
P value
0.051
0.378
0.735
0.924
0.969
rs73021485
GT
55.29 ± 0.67
25.73 ± 0.23
0.95 ± 0.01
128.21 ± 1.06
76.30 ± 0.61
GG
53.87 ± 0.78
26.17 ± 0.25
0.94 ± 0.00
127.35 ± 1.30
76.17 ± 0.69
TT
54.29 ± 1.53
25.81 ± 0.50
0.93 ± 0.01
128.13 ± 2.38
75.75 ± 1.08
P value
0.058
0.425
0.577
0.867
0.923
rs2920502
GC
54.15 ± 0.71
25.93 ± 0.22
0.95 ± 0.01
128.24 ± 1.19
76.62 ± 0.64
CC
53.97 ± 0.72
25.77 ± 0.24
0.93 ± 0.00
127.89 ± 1.12
75.98 ± 0.61
GG
50.36 ± 1.85
26.72 ± 0.61
0.94 ± 0.00
126.33 ± 2.78
75.10 ± 1.43
P value
0.098
0.274
0.352
0.803
0.561
rs17029007
GG
53.76 ± 0.57
25.92 ± 0.19
0.94 ± 0.01
128.17 ± 0.86
76.07 ± 0.47
GA
53.74 ± 1.03
25.87 ± 0.34
0.94 ± 0.01
126.59 ± 1.86
77.01 ± 0.96
AA
50.75 ± 4.20
27.74 ± 1.04
0.94 ± 0.01
128.88 ± 10.20
68.75 ± 2.56
P value
0.768
0.399
0.992
0.698
0.069
rs3856806
CC
52.84 ± 0.62
25.84 ± 0.20
0.94 ± 0.00
128.17 ± 1.01
76.17 ± 0.53
TC
54.77 ± 0.84
26.03 ± 0.30
0.95 ± 0.01
127.02 ± 1.26
76.35 ± 0.73
TT
55.45 ± 2.47
26.33 ± 0.71
0.93 ± 0.01
130.75 ± 5.31
75.50 ± 2.73
P value
0.052
0.765
0.613
0.616
0.932


### 3.5. Association of Genotype with Insulin Secretion Function, Blood Glucose, and Lipid Profiles

Subjects with TT genotype of rs649446 or with AA genotype of rs7109266 in UCP2 had higher fasting insulin, HOMA-IR, and HOMA-β than subjects with other genotypes, but blood glucose profiles including fasting and 2 hr postprandial glucose were not significantly different among genotypes (Table 8). There was no significant difference in glucose profiles and insulin secretion in other loci of UCP2 (Table 8). The serum lipid TC, TG, HDL-C, and LDL-C were not significantly different among genotypes of selected SNPs in UCP2 (Table 9).Table 8
Association of genotype with insulin secretion function and blood glucose in UCP2.
Genotype
HbA1c%
FPG (mmol/l)
Glu30′ (mmol/l)
Glu60′ (mmol/l)
Glu120′ (mmol/l)
Ins0′ (μIU/ml)
Ins30′ (μIU/ml)
Ins60′ (μIU/ml)
Ins120′ (μIU/ml)
HOMA-IR
HOMA-β
DI30
DI120
rs660339
GG
6.06 ± 0.11
6.89 ± 0.21
11.37 ± 0.31
11.26 ± 0.42
9.66 ± 0.46
11.79 ± 0.77
78.32 ± 5.24
78.70 ± 4.76
51.97 ± 3.60
3.43 ± 0.23
88.43 ± 5.73
414.74 ± 23.47
536.01 ± 24.80
AA
5.99 ± 0.11
6.80 ± 0.23
11.23 ± 0.32
11.38 ± 0.44
9.55 ± 0.48
12.48 ± 1.16
69.12 ± 4.91
74.85 ± 4.53
57.01 ± 3.97
3.92 ± 0.43
90.93 ± 6.62
383.99 ± 27.42
514.93 ± 28.03
GA
6.01 ± 0.07
6.68 ± 0.13
11.01 ± 0.21
10.92 ± 0.29
8.83 ± 0.28
12.29 ± 0.61
71.72 ± 3.28
78.81 ± 3.47
58.45 ± 3.07
3.97 ± 0.33
89.23 ± 3.38
420.51 ± 17.56
587.96 ± 34.53
P value
0.894
0.677
0.592
0.633
0.187
0.849
0.379
0.803
0.412
0.524
0.95
0.518
0.294
rs659366
CC
6.05 ± 0.11
6.88 ± 0.22
11.37 ± 0.32
11.28 ± 0.43
9.68 ± 0.47
11.80 ± 0.79
77.38 ± 5.27
78.68 ± 4.82
52.52 ± 3.68
3.43 ± 0.24
88.80 ± 5.86
410.35 ± 23.43
535.63 ± 25.18
TT
5.93 ± 0.11
6.74 ± 0.22
11.09 ± 0.31
11.07 ± 0.43
9.31 ± 0.46
12.56 ± 1.12
71.34 ± 5.05
75.55 ± 4.77
58.35 ± 4.31
3.88 ± 0.42
92.55 ± 6.54
395.63 ± 27.34
526.68 ± 27.42
TC
6.04 ± 0.07
6.71 ± 0.14
11.07 ± 0.21
11.04 ± 0.29
8.92 ± 0.28
12.24 ± 0.60
71.39 ± 3.25
78.60 ± 3.41
57.57 ± 2.98
3.99 ± 0.33
88.32 ± 3.34
418.18 ± 17.59
583.49 ± 34.57
P value
0.677
0.796
0.706
0.889
0.327
0.84
0.56
0.871
0.526
0.518
0.821
0.777
0.43
rs649446
CC
6.06 ± 0.09
6.84 ± 0.16
11.24 ± 0.24
11.20 ± 0.33
9.46 ± 0.34
12.05 ± 0.55
75.14 ± 3.99
79.96 ± 3.95
56.72 ± 3.32
3.61 ± 0.18
90.91 ± 4.38
404.52 ± 18.16
535.54 ± 20.23
TT
6.06 ± 0.16
6.90 ± 0.32
11.50 ± 0.47
11.65 ± 0.65
10.06 ± 0.73
16.71 ± 2.96
74.87 ± 7.74
83.49 ± 7.86
67.33 ± 7.15
5.78 ± 1.53
108.66 ± 12.90
335.15 ± 26.95
466.10 ± 31.42
TC
5.98 ± 0.07
6.67 ± 0.15
11.01 ± 0.21
10.93 ± 0.29
8.81 ± 0.30
11.38 ± 0.50
70.59 ± 3.32
75.06 ± 3.24
54.06 ± 2.76
3.59 ± 0.23
83.77 ± 3.10
433.37 ± 19.96
599.24 ± 37.91
P value
0.765
0.676
0.581
0.567
0.156
0.003∗
0.655
0.469
0.176
0.006∗
0.026∗
0.066
0.096
rs7109266
GG
6.07 ± 0.09
6.86 ± 0.16
11.24 ± 0.24
11.24 ± 0.33
9.48 ± 0.34
12.03 ± 0.55
74.80 ± 3.96
79.68 ± 3.92
56.77 ± 3.29
3.62 ± 0.18
90.40 ± 4.35
402.30 ± 18.07
533.33 ± 20.14
AA
6.07 ± 0.17
6.96 ± 0.33
11.58 ± 0.50
11.79 ± 0.67
10.16 ± 0.77
17.11 ± 3.12
76.19 ± 8.10
84.59 ± 8.20
68.44 ± 7.51
5.98 ± 1.61
109.45 ± 13.52
334.04 ± 28.21
461.60 ± 33.00
GA
5.97 ± 0.07
6.64 ± 0.15
10.99 ± 0.21
10.87 ± 0.29
8.78 ± 0.30
11.37 ± 0.49
70.64 ± 3.31
75.15 ± 3.23
53.93 ± 2.75
3.57 ± 0.23
84.30 ± 3.09
434.69 ± 19.84
601.13 ± 37.71
P value
0.601
0.502
0.49
0.401
0.107
0.001∗
0.656
0.449
0.137
0.003∗
0.031∗
0.06
0.078
rs591758
GG
6.05 ± 0.11
6.87 ± 0.21
11.33 ± 0.31
11.24 ± 0.42
9.66 ± 0.46
11.91 ± 0.79
77.66 ± 5.22
78.40 ± 4.75
52.65 ± 3.63
3.46 ± 0.23
89.39 ± 5.7
410.86 ± 23.20
534.33 ± 24.89
CC
5.94 ± 0.11
6.78 ± 0.23
11.11 ± 0.30
11.11 ± 0.41
9.32 ± 0.45
13.43 ± 1.36
73.30 ± 5.06
78.32 ± 4.83
59.57 ± 4.33
4.45 ± 0.70
94.35 ± 6.42
392.34 ± 26.39
525.85 ± 26.71
CG
6.04 ± 0.07
6.70 ± 0.14
11.07 ± 0.21
11.04 ± 0.29
8.91 ± 0.29
11.79 ± 0.48
70.32 ± 3.25
77.56 ± 3.43
56.98 ± 3.00
3.71 ± 0.21
87.12 ± 3.36
419.88 ± 17.95
585.93 ± 35.37
P value
0.73
0.775
0.77
0.92
0.343
0.322
0.457
0.987
0.485
0.209
0.57
0.682
0.381
rs586773
AA
6.05 ± 0.11
6.87 ± 0.21
11.33 ± 0.31
11.24 ± 0.42
9.66 ± 0.46
11.91 ± 0.79
77.66 ± 5.22
78.40 ± 4.75
52.65 ± 3.63
3.46 ± 0.23
89.39 ± 5.79
410.86 ± 23.20
534.33 ± 24.89
TT
5.94 ± 0.11
6.78 ± 0.23
11.11 ± 0.30
11.11 ± 0.41
9.32 ± 0.45
13.43 ± 1.36
73.30 ± 5.06
78.32 ± 4.83
59.57 ± 4.33
4.45 ± 0.70
94.35 ± 6.42
392.34 ± 26.39
525.85 ± 26.71
AT
6.04 ± 0.07
6.71 ± 0.14
11.10 ± 0.21
11.09 ± 0.30
8.93 ± 0.29
11.80 ± 0.49
70.25 ± 3.29
77.60 ± 3.46
57.04 ± 3.03
3.73 ± 0.22
86.95 ± 3.39
418.26 ± 18.06
583.80 ± 35.73
P value
0.711
0.804
0.798
0.952
0.365
0.33
0.453
0.988
0.484
0.214
0.557
0.713
0.413
rs34408426
AA
6.05 ± 0.11
6.87 ± 0.21
11.33 ± 0.31
11.24 ± 0.42
9.66 ± 0.46
11.91 ± 0.79
77.66 ± 5.22
78.40 ± 4.75
52.65 ± 3.63
3.46 ± 0.23
89.39 ± 5.79
410.86 ± 23.20
534.33 ± 24.89
GG
5.94 ± 0.11
6.78 ± 0.23
11.11 ± 0.30
11.11 ± 0.41
9.32 ± 0.45
13.43 ± 1.36
73.30 ± 5.06
78.32 ± 4.83
59.57 ± 4.33
4.45 ± 0.70
94.35 ± 6.42
392.34 ± 26.39
525.85 ± 26.71
AG
6.03 ± 0.07
6.67 ± 0.13
11.02 ± 0.21
11.00 ± 0.29
8.90 ± 0.29
11.81 ± 0.49
70.71 ± 3.28
77.99 ± 3.46
57.37 ± 3.02
3.71 ± 0.21
87.48 ± 3.38
421.79 ± 18.08
588.64 ± 35.70
P value
0.747
0.698
0.694
0.882
0.331
0.332
0.499
0.997
0.471
0.209
0.602
0.646
0.35
rs3019463
CC
6.03 ± 0.11
6.85 ± 0.21
11.30 ± 0.31
11.19 ± 0.42
9.60 ± 0.46
11.91 ± 0.79
77.96 ± 5.25
78.59 ± 4.78
52.63 ± 3.66
3.45 ± 0.24
89.77 ± 5.82
413.23 ± 23.25
537.30 ± 24.89
TT
5.96 ± 0.11
6.82 ± 0.23
11.15 ± 0.31
11.23 ± 0.42
9.40 ± 0.46
13.54 ± 1.39
72.78 ± 5.11
79.20 ± 4.90
60.35 ± 4.41
4.51 ± 0.72
94.20 ± 6.53
381.46 ± 25.95
518.95 ± 26.89
TC
6.02 ± 0.07
6.67 ± 0.14
11.08 ± 0.22
11.00 ± 0.30
8.91 ± 0.29
11.75 ± 0.50
70.74 ± 3.30
78.34 ± 3.50
57.65 ± 3.08
3.70 ± 0.22
87.13 ± 3.38
425.19 ± 18.44
593.35 ± 36.28
P value
0.864
0.74
0.847
0.883
0.369
0.278
0.473
0.99
0.41
0.172
0.59
0.39
0.275
∗P<0.05. DI30 (early-phase disposition index of insulin secretion) = [InsAUC30/GluAUC30] × ISIM, DI120 (total-phase disposition of insulin secretion) = [InsAUC120/GluAUC120] × ISIM.Table 9
Association of genotype with lipid profiles in UCP2.
Genotype
TC (mmol/l)
TG (mmol/l)
HDL-C (mmol/l)
LDL-C (mmol/l)
TG/HDL-C
rs660339
GG
5.44 ± 0.09
1.81 ± 0.16
1.35 ± 0.04
2.82 ± 0.06
1.45 ± 0.12
AA
5.49 ± 0.10
1.72 ± 0.10
1.30 ± 0.03
2.85 ± 0.07
1.45 ± 0.10
GA
5.39 ± 0.06
2.16 ± 0.41
1.29 ± 0.02
2.83 ± 0.04
1.79 ± 0.32
P value
0.702
0.678
0.359
0.93
0.627
rs659366
CC
5.44 ± 0.09
1.73 ± 0.14
1.34 ± 0.05
2.83 ± 0.06
1.41 ± 0.11
TT
5.49 ± 0.10
1.69 ± 0.10
1.30 ± 0.03
2.86 ± 0.07
1.43 ± 0.09
TC
5.39 ± 0.06
2.22 ± 0.42
1.29 ± 0.02
2.82 ± 0.04
1.82 ± 0.32
P value
0.672
0.536
0.405
0.919
0.519
rs649446
CC
5.35 ± 0.07
2.34 ± 0.53
1.34 ± 0.03
2.77 ± 0.05
1.85 ± 0.40
TT
5.61 ± 0.16
1.66 ± 0.12
1.29 ± 0.04
2.95 ± 0.12
1.40 ± 0.12
TC
5.44 ± 0.06
1.72 ± 0.07
1.28 ± 0.02
2.86 ± 0.05
1.48 ± 0.07
P value
0.254
0.39
0.257
0.157
0.544
rs7109266
GG
5.35 ± 0.07
2.33 ± 0.52
1.34 ± 0.03
2.77 ± 0.05
1.84 ± 0.40
AA
5.56 ± 0.17
1.63 ± 0.12
1.28 ± 0.04
2.93 ± 0.12
1.39 ± 0.13
GA
5.45 ± 0.06
1.73 ± 0.07
1.28 ± 0.02
2.87 ± 0.04
1.48 ± 0.07
P value
0.351
0.404
0.249
0.206
0.563
rs591758
GG
5.44 ± 0.09
1.72 ± 0.13
1.34 ± 0.04
2.84 ± 0.06
1.41 ± 0.11
CC
5.49 ± 0.10
1.69 ± 0.10
1.30 ± 0.03
2.86 ± 0.07
1.43 ± 0.09
CG
5.38 ± 0.06
2.23 ± 0.43
1.29 ± 0.02
2.82 ± 0.04
1.83 ± 0.33
P value
0.633
0.509
0.497
0.846
0.501
rs586773
AA
5.44 ± 0.09
1.72 ± 0.13
1.34 ± 0.04
2.84 ± 0.06
1.41 ± 0.11
TT
5.49 ± 0.10
1.69 ± 0.10
1.30 ± 0.03
2.86 ± 0.07
1.43 ± 0.09
AT
5.38 ± 0.06
2.23 ± 0.43
1.30 ± 0.02
2.82 ± 0.04
1.82 ± 0.33
P value
0.635
0.515
0.515
0.855
0.508
rs34408426
AA
5.44 ± 0.09
1.72 ± 0.13
1.34 ± 0.04
2.84 ± 0.06
1.41 ± 0.11
GG
5.49 ± 0.10
1.69 ± 0.10
1.30 ± 0.03
2.86 ± 0.07
1.43 ± 0.09
AG
5.37 ± 0.06
2.20 ± 0.43
1.29 ± 0.02
2.81 ± 0.04
1.82 ± 0.33
P value
0.536
0.554
0.449
0.829
0.521
rs3019463
CC
5.43 ± 0.09
1.72 ± 0.14
1.34 ± 0.05
2.83 ± 0.06
1.41 ± 0.11
TT
5.51 ± 0.10
1.69 ± 0.10
1.30 ± 0.03
2.87 ± 0.07
1.43 ± 0.09
TC
5.38 ± 0.07
2.23 ± 0.44
1.30 ± 0.02
2.81 ± 0.04
1.82 ± 0.34
P value
0.538
0.522
0.561
0.744
0.527Subjects with GG genotype of rs2920502 in PPARγ had better HbA1c, 0 min, 30 min, and 120 min blood glucose, increased 60 min and 120 min insulin secretion after taking 75 g glucose, and lower serum TC, TG, and LDL-C compared to GC/CC genotypes (Table 10); the HOMA-IR in GG genotype was lower than GC/CC genotypes. Subjects with TT genotype of rs2920503 in PPARγ had better HbA1c, 0 min, 30 min, 60 min, and 120 min blood glucose and had increased serum insulin in 120 min after taking 75 g glucose compared to TC/CC genotypes (Table 10). Subjects with TT genotypes of rs3856806 had higher fasting blood glucose than TC/CC genotypes, and postprandial blood glucose and insulin secretion were not significantly different among genotypes. The blood glucose at 0 min, 30 min, 60 min, and 120 min after taking 75 g glucose in subjects with AA/GG genotype of rs79310821 were better than subjects with GA genotype. The blood glucose at 0 min, 30 min, 60 min, and 120 min after taking 75 g glucose in subjects with TT/GG genotype of rs79310821 was better than that in subjects with TG genotype, and index of insulin secretion-HOMA-β, DI30, and DI120 were higher in TT/GG genotype than in TG genotype. The serum lipid profiles were not significantly different in other loci in PPARγ (Table 11).Table 10
Association of genotype with insulin secretion function and blood glucose in PPARγ.
Genotype
HbA1c%
FPG (mmol/l)
Glu30′ (mmol/l)
Glu60′ (mmol/l)
Glu120′ (mmol/l)
Ins0′ (μIU/ml)
Ins30′ (μIU/ml)
Ins60′ (μIU/ml)
Ins120′ (μIU/ml)
HOMA-IR
HOMA-β
DI30
DI120
rs2920503
CC
6.06 ± 0.08
6.84 ± 0.16
11.34 ± 0.23
11.16 ± 0.32
9.17 ± 0.33
11.59 ± 0.57
73.21 ± 3.49
74.50 ± 3.44
50.88 ± 2.74
3.69 ± 0.33
85.26 ± 3.25
423.09 ± 18.98
577.78 ± 37.56
CT
6.10 ± 0.08
6.87 ± 0.16
11.32 ± 0.25
11.55 ± 0.33
9.69 ± 0.35
13.10 ± 0.86
72.05 ± 3.91
82.18 ± 3.95
53.29 ± 6.52
4.15 ± 0.32
92.27 ± 5.27
387.24 ± 19.31
527.33 ± 20.78
TT
5.59 ± 0.11
5.98 ± 0.12
9.82 ± 0.29
9.33 ± 0.44
7.72 ± 0.35
12.05 ± 0.92
78.26 ± 8.06
78.79 ± 6.95
62.76 ± 3.49
3.31 ± 0.29
101.01 ± 6.49
460.64 ± 35.70
592.80 ± 31.89
P value
0.019∗
0.039∗
0.012∗
0.01∗
0.034∗
0.295
0.77
0.332
0.024∗
0.405
0.186
0.178
0.434
rs73813168
AA
6.02 ± 0.06
6.77 ± 0.12
11.16 ± 0.18
11.14 ± 0.24
9.32 ± 0.25
11.90 ± 0.41
71.68 ± 2.77
78.64 ± 2.76
57.65 ± 2.39
3.67 ± 0.17
87.72 ± 2.87
402.67 ± 13.84
556.93 ± 24.92
GA
6.04 ± 0.11
6.78 ± 0.23
11.21 ± 0.32
11.13 ± 0.46
8.87 ± 0.46
12.14 ± 1.04
75.88 ± 5.27
76.82 ± 5.22
52.35 ± 4.13
3.99 ± 0.63
89.11 ± 5.46
439.29 ± 29.79
570.00 ± 31.05
GG
5.85 ± 0.12
5.99 ± 0.19
11.09 ± 0.80
10.54 ± 1.91
6.21 ± 1.12
12.59 ± 3.34
99.09 ± 51.54
73.55 ± 20.46
43.04 ± 15.77
3.35 ± 0.86
102.59 ± 30.85
414.77 ± 154.51
513.87 ± 66.05
P value
0.947
0.814
0.99
0.97
0.336
0.96
0.506
0.939
0.479
0.777
0.864
0.477
0.946
rs79310821
GA
6.16 ± 0.08
7.08 ± 0.18
11.59 ± 0.24
11.76 ± 0.33
9.92 ± 0.35
12.81 ± 0.87
68.22 ± 3.24
76.64 ± 3.47
56.29 ± 3.03
3.92 ± 0.36
83.64 ± 3.79
412.36 ± 20.56
522.64 ± 21.28
GG
5.91 ± 0.08
6.52 ± 0.13
10.80 ± 0.22
10.68 ± 0.31
8.71 ± 0.31
12.14 ± 0.48
76.31 ± 3.96
78.00 ± 3.63
56.77 ± 3.09
3.80 ± 0.28
91.79 ± 4.26
403.67 ± 16.44
586.01 ± 36.88
AA
5.89 ± 0.11
6.39 ± 0.17
10.67 ± 0.37
10.13 ± 0.51
8.30 ± 0.52
10.14 ± 0.62
78.61 ± 8.39
80.96 ± 8.19
56.84 ± 6.70
3.50 ± 0.33
105.43 ± 8.54
442.89 ± 46.25
583.89 ± 43.95
P value
0.055
0.015∗
0.029∗
0.012∗
0.009∗
0.192
0.21
0.856
0.993
0.855
0.069
0.695
0.301
rs73021485
GT
6.14 ± 0.08
7.06 ± 0.18
11.59 ± 0.24
11.74 ± 0.32
9.86 ± 0.34
12.32 ± 0.82
68.11 ± 3.22
76.83 ± 3.47
56.48 ± 3.03
4.14 ± 0.40
82.09 ± 3.90
373.32 ± 17.21
509.27 ± 19.15
GG
5.91 ± 0.08
6.51 ± 0.13
10.80 ± 0.22
10.67 ± 0.31
8.67 ± 0.31
12.17 ± 0.48
76.73 ± 3.97
78.64 ± 3.66
56.42 ± 3.09
3.56 ± 0.17
96.67 ± 4.06
438.29 ± 20.28
564.71 ± 19.91
TT
5.97 ± 0.12
6.51 ± 0.19
10.74 ± 0.39
10.30 ± 0.55
8.59 ± 0.57
11.87 ± 1.25
78.18 ± 8.14
80.13 ± 7.86
56.92 ± 6.44
3.52 ± 0.48
91.36 ± 9.05
460.55 ± 37.27
724.86 ± 133.17I
P value
0.112
0.028∗
0.031∗
0.019∗
0.021∗
0.949
0.186
0.89
0.997
0.363
0.041∗
0.018∗
0.004∗
rs2920502
GC
6.20 ± 0.10
7.11 ± 0.19
11.58 ± 0.26
11.67 ± 0.35
8.98 ± 0.28
11.75 ± 0.64
71.26 ± 3.88
73.99 ± 3.59
54.91 ± 5.56
3.56 ± 0.17
84.54 ± 4.31
436.29 ± 20.24
561.96 ± 19.83
CC
6.02 ± 0.06
7.15 ± 0.11
11.85 ± 0.19
11.76 ± 0.27
9.76 ± 0.38
12.55 ± 0.73
73.30 ± 3.47
70.05 ± 3.57
50.38 ± 2.98
4.33 ± 0.42
96.17 ± 4.05
373.56 ± 17.36
507.65 ± 19.37
GG
5.74 ± 0.11
6.25 ± 0.24
10.72 ± 0.46
10.43 ± 0.64
7.82 ± 0.52
12.68 ± 1.00
80.64 ± 7.31
85.24 ± 7.56
62.44 ± 3.16
2.81 ± 0.19
83.97 ± 6.40
470.32 ± 37.73
743.78 ± 136.80
P value
0.009∗
0.01∗
0.049∗
0.068
0.032∗
0.663
0.579
0.015∗
0.019∗
0.041∗
0.104
0.015∗
0.002∗
rs17029007
GG
6.01 ± 0.06
6.77 ± 0.12
11.14 ± 0.18
11.12 ± 0.24
9.30 ± 0.25
12.18 ± 0.50
71.74 ± 2.75
78.71 ± 2.74
57.73 ± 2.40
3.76 ± 0.19
89.10 ± 3.15
402.92 ± 13.76
557.25 ± 24.78
GA
6.00 ± 0.11
6.76 ± 0.24
11.23 ± 0.33
11.16 ± 0.48
8.98 ± 0.49
12.35 ± 1.09
75.56 ± 5.32
76.13 ± 5.39
52.52 ± 4.26
4.05 ± 0.65
90.98 ± 5.74
437.31 ± 30.27
563.96 ± 31.32
AA
6.26 ± 0.50
6.01 ± 0.16
10.68 ± 0.62
9.78 ± 1.21
6.35 ± 0.79
10.79 ± 1.81
111.10 ± 30.97
79.93 ± 13.81
49.28 ± 10.87
2.87 ± 0.47
89.40 ± 17.08
514.60 ± 108.50
642.35 ± 83.52
P value
0.837
0.684
0.903
0.741
0.231
0.92
0.138
0.904
0.532
0.725
0.96
0.319
0.873
rs3856806
CC
5.96 ± 0.06
6.57 ± 0.11
10.92 ± 0.18
10.78 ± 0.25
8.93 ± 0.26
11.87 ± 0.41
75.61 ± 3.14
77.37 ± 3.07
56.12 ± 2.58
3.51 ± 0.14
91.40 ± 3.05
432.97 ± 16.46
582.14 ± 29.37
TC
6.12 ± 0.10
7.11 ± 0.22
11.58 ± 0.29
11.68 ± 0.39
9.69 ± 0.40
12.75 ± 1.08
69.35 ± 4.21
78.76 ± 4.16
56.36 ± 3.54
4.33 ± 0.53
86.40 ± 5.67
377.68 ± 20.08
518.92 ± 22.77
TT
6.08 ± 0.21
7.88 ± 0.47
11.33 ± 0.82
11.60 ± 1.24
9.27 ± 1.42
12.59 ± 2.01
62.15 ± 8.81
77.54 ± 11.07
58.24 ± 12.15
4.36 ± 1.22
82.71 ± 11.35
358.63 ± 57.56
506.31 ± 62.29
P value
0.326
0.047∗
0.127
0.121
0.257
0.65
0.338
0.964
0.981
0.155
0.619
0.085
0.302
∗P<0.05. DI30 (early-phase disposition index of insulin secretion) = [InsAUC30/GluAUC30] × ISIM, DI120 (total-phase disposition of insulin secretion) = [InsAUC120/GluAUC120] × ISIM.Table 11
Association of genotype with lipid profiles in PPARγ.
Genotype
TC (mmol/l)
TG (mmol/l)
HDL-C (mmol/l)
LDL-C (mmol/l)
TG/HDL-C
rs2920503
CC
5.51 ± 0.07
2.25 ± 0.46
1.32 ± 0.03
2.87 ± 0.05
1.81 ± 0.35
CT
5.36 ± 0.08
1.72 ± 0.10
1.28 ± 0.02
2.83 ± 0.05
1.44 ± 0.08
TT
5.26 ± 0.11
1.78 ± 0.16
1.33 ± 0.08
2.66 ± 0.08
1.56 ± 0.17
P value
0.154
0.53
0.457
0.146
0.594
rs73813168
AA
5.37 ± 0.05
1.78 ± 0.08
1.29 ± 0.02
2.80 ± 0.03
1.49 ± 0.06
GA
5.44 ± 0.11
2.67 ± 0.94
1.37 ± 0.05
2.97 ± 0.08
2.11 ± 0.72
GG
5.26 ± 0.59
1.36 ± 0.23
1.27 ± 0.13
2.73 ± 0.44
1.06 ± 0.12
P value
0.054
0.245
0.164
0.057
0.313
rs79310821
GA
5.49 ± 0.06
1.70 ± 0.08
1.30 ± 0.02
2.88 ± 0.04
1.41 ± 0.07
GG
5.42 ± 0.08
1.81 ± 0.11
1.33 ± 0.03
2.83 ± 0.05
1.49 ± 0.09
AA
5.20 ± 0.12
1.88 ± 0.24
1.25 ± 0.03
2.67 ± 0.09
1.61 ± 0.18
P value
0.147
0.629
0.283
0.109
0.484
rs73021485
GT
5.49 ± 0.06
1.70 ± 0.08
1.29 ± 0.02
2.88 ± 0.04
1.41 ± 0.07
GG
5.41 ± 0.08
2.30 ± 0.52
1.33 ± 0.03
2.83 ± 0.05
1.87 ± 0.40
TT
5.19 ± 0.12
1.85 ± 0.23
1.26 ± 0.03
2.67 ± 0.09
1.58 ± 0.18
P value
0.144
0.452
0.313
0.109
0.464
rs2920502
GC
5.48 ± 0.07
2.39 ± 0.08
1.31 ± 0.02
2.90 ± 0.05
1.82 ± 0.08
CC
5.72 ± 0.20
4.35 ± 2.43
1.28 ± 0.02
2.98 ± 0.15
3.40 ± 0.07
GG
5.32 ± 0.06
1.73 ± 0.11
1.42 ± 0.12
2.75 ± 0.04
1.22 ± 0.26
P value
0.034∗
0.004∗∗
0.07
0.031∗
0.006∗∗
rs17029007
GG
5.37 ± 0.05
1.78 ± 0.07
1.29 ± 0.02
2.80 ± 0.03
1.49 ± 0.06
GA
5.58 ± 0.10
2.70 ± 0.98
1.38 ± 0.05
2.91 ± 0.07
2.13 ± 0.75
AA
5.55 ± 0.45
1.46 ± 0.23
1.29 ± 0.09
2.94 ± 0.31
1.15 ± 0.17
P value
0.165
0.233
0.071
0.334
0.301
rs3856806
CC
5.42 ± 0.06
2.04 ± 0.35
1.30 ± 0.02
2.84 ± 0.04
1.71 ± 0.27
TC
5.44 ± 0.08
1.91 ± 0.16
1.32 ± 0.02
2.81 ± 0.05
1.52 ± 0.11
TT
5.39 ± 0.27
1.48 ± 0.17
1.25 ± 0.06
2.88 ± 0.16
1.27 ± 0.16
P value
0.971
0.882
0.741
0.846
0.811
∗P<0.05 and ∗∗P<0.01.


### 3.6. Association of Genotype with Inflammation

There was no significant difference in TNF-α among genotypes in UCP2. The serum IL-6 was higher in subjects with TT genotype of rs660339 than in GG/GA genotype, and IL-6 was higher in subjects with TT genotype of rs649446 than in CC/TC genotype (Table 12). There was no significant difference in inflammation indicators among genotypes in PPARγ (Table 13).Table 12
Association of genotype with inflammation in UCP2.
Genotype
TNF-α (fmol/ml)
IL-6 (pg/ml)
rs660339
GG
23.44 ± 0.86
1.62 ± 0.08
AA
22.25 ± 0.95
1.42 ± 0.10
GA
22.40 ± 0.57
1.70 ± 0.05
P value
0.527
0.034∗
rs659366
CC
23.31 ± 0.87
1.64 ± 0.08
TT
22.10 ± 0.94
1.46 ± 0.10
TC
22.55 ± 0.57
1.67 ± 0.05
P value
0.598
0.111
rs649446
CC
23.28 ± 0.66
1.68 ± 0.06
TT
20.97 ± 1.48
1.95 ± 0.16
TC
22.37 ± 0.59
1.65 ± 0.06
P value
0.257
0.001∗∗
rs7109266
GG
23.22 ± 0.66
1.68 ± 0.06
AA
20.78 ± 1.50
1.52 ± 0.17
GA
22.45 ± 0.60
1.65 ± 0.06
P value
0.257
0.063
rs591758
GG
23.37 ± 0.86
1.64 ± 0.08
CC
21.81 ± 0.89
1.46 ± 0.10
CG
22.65 ± 0.59
1.68 ± 0.06
P value
0.439
0.111
rs586773
AA
23.37 ± 0.86
1.64 ± 0.08
TT
21.81 ± 0.89
1.46 ± 0.10
AT
22.61 ± 0.59
1.67 ± 0.06
P value
0.437
0.12
rs34408426
AA
23.37 ± 0.86
1.64 ± 0.08
GG
21.81 ± 0.89
1.46 ± 0.10
AG
22.56 ± 0.59
1.67 ± 0.06
P value
0.434
0.124
rs3019463
CC
23.34 ± 0.86
1.64 ± 0.08
TT
22.15 ± 0.89
1.47 ± 0.10
TC
22.58 ± 0.60
1.66 ± 0.06
P value
0.606
0.17
∗P<0.05 and ∗∗P<0.01. TNF-α: tumor necrosis factor-α; IL-6: interleukine-6. IL-6 has been nature logarithm transformed.Table 13
Association of genotype with inflammation in PPARγ.
Genotype
TNF-α (fmol/ml)
IL-6 (pg/ml)
rs2920503
CC
22.60 ± 0.64
57.77 ± 0.96
CT
22.42 ± 0.62
60.82 ± 1.02
TT
23.51 ± 1.51
60.76 ± 2.40
P value
0.778
0.081
rs73813168
AA
23.11 ± 0.49
1.63 ± 0.05
GA
21.68 ± 0.88
1.56 ± 0.09
GG
16.44 ± 4.27
1.76 ± 0.27
P value
0.164
0.742
rs79310821
GA
23.02 ± 0.63
1.64 ± 0.06
GG
22.34 ± 0.66
1.59 ± 0.07
AA
22.29 ± 1.25
1.64 ± 0.11
P value
0.723
0.844
rs73021485
GT
22.99 ± 0.63
1.64 ± 0.06
GG
22.32 ± 0.67
1.59 ± 0.07
TT
22.28 ± 1.22
1.60 ± 0.11
P value
0.735
0.81
rs2920502
GC
22.78 ± 0.67
1.57 ± 0.06
CC
22.70 ± 0.61
1.68 ± 0.06
GG
21.41 ± 1.41
1.45 ± 0.14
P value
0.665
0.231
rs17029007
GG
23.03 ± 0.49
1.63 ± 0.05
GA
21.70 ± 0.87
1.55 ± 0.10
AA
17.39 ± 3.82
1.80 ± 0.22
P value
0.137
0.634
rs3856806
CC
22.62 ± 0.52
1.58 ± 0.05
TC
22.63 ± 0.79
1.69 ± 0.07
TT
21.87 ± 2.18
1.58 ± 0.24
P value
0.949
0.419
TNF-α: tumor necrosis factor-α; IL-6: interleukine-6. IL-6 has been nature logarithm transformed.


## 3.1. Allele Frequency Analysis

All loci conformed to Hardy-Weinberg equilibrium as shown in Supplementary Table1. There was no significant difference in allele frequency of each SNP in UCP2 between prediabetes/diabetes group and normal glucose tolerance group (Table 3). In PPARγ, the G allele in rs2920502 decreased the risk of diabetes (OR: 0.818, 95%CI: 0.526–0.969, P=0.042), the T allele in rs3856806 increased the risk of diabetes (OR: 1.46, 95%CI: 1.055–2.017, P=0.022) (Table 3). In UCP2, there was no significant difference between alleles in each SNP.Table 3
Allele frequency analysis between prediabetes/diabetes group and normal blood glucose group.
Gene name
SNP number
Allele
Prediabetes/diabetes group
Normal blood glucose group
Odd ratio (OR)
95% confidence interval (CI)
P value
(N)
(N)
Low
High
UCP2
rs660339
A
346
187
0.997
0.775
1.281
0.9778
G
388
209
rs659366
T
351
192
0.973
0.758
1.248
0.828
C
383
204
rs649446
T
244
136
0.949
0.728
1.238
0.700
C
490
260
rs586773
T
351
192
0.959
0.750
1.227
0.741
A
381
200
rs34408426
G
350
193
0.959
0.750
1.227
0.738
A
380
201
rs7109266
A
240
135
0.935
0.715
1.222
0.624
G
494
261
rs3019463
T
343
188
0.978
0.763
1.253
0.861
C
373
200
rs591758
C
352
194
0.959
0.750
1.227
0.738
G
382
202
PPARγ
rs2920503
A
213
126
0.918
0.708
1.191
0.519
G
499
270
rs73813168
G
80
58
0.693
0.474
1.012
0.057
A
646
334
rs79310821
A
265
126
1.2
0.924
1.560
0.172
G
467
266
rs73021485
T
271
127
1.241
0.957
1.609
0.104
G
463
269
rs2920502
G
214
132
0.818
0.526
0.969
0.042∗
C
516
262
rs17029007
A
81
60
0.699
0.486
1.005
0.053
G
645
336
rs3856806
T
163
66
1.460
1.055
2.017
0.022∗
C
567
330
∗P<0.05.


## 3.2. Genotype Analysis

The association of SNPs with prediabetes/diabetes was assessed by crosstab test and logistic regression after adjustment for age and sex. In PPARγ, the frequency of GG genotype in rs2920502 was significantly lower in prediabetes/diabetes subjects (6.85%) than in the normal glucose tolerance subjects (12.69%); logistic regression analysis revealed that subjects with GG genotype of rs2920502 in PPARγ had less risk for prediabetes/diabetes compared to CC genotype (odd ratio (OR): 0.715; 95% confidence interval (CI): 0.527–0.97, P=0.031). The frequency of TT genotype in rs3856806 was significantly higher in prediabetes/diabetes subjects than in the normal glucose tolerance subjects; logistic regression analysis showed that subjects with TT genotype of rs3856806 in PPARγ had higher risk for diabetes compared to CC (OR: 1.58, 95%CI: 1.104–2.761, P=0.032). Furthermore, we, respectively, performed a logistic regression analysis under a recessive inheritance model (GG versus GC + CC) in rs2920502 and a dominant inheritance model (TT + TC/CC) in rs3856806; the regression showed that the odd ratio for GG versus GC + CC in rs2920502 was 0.506 (95%CI: 0.282–0.906, P=0.022) and the odd ratio for TT + TC/CC in rs3856806 was 1.479 (95%CI: 1.026–2.133, P=0.036). These were in accordance with the allele frequency analysis, which implied that G allele carriers in rs2920502 were less susceptible to develop diabetes and T allele carriers in rs3856806 were more susceptible to develop diabetes. No significant difference was found at other loci in PPARγ (Table 4). There was no significant difference in the genotype of each SNPs in UCP2 (Table 4).Table 4
Genotype analysis between prediabetes/diabetes group and normal blood glucose group.
Gene name
SNP number
Genotype
Prediabetes/diabetes group
Normal blood glucose group
Odd ratio (OR)
95% confidence interval (CI)
P value
Low
High
UCP2
rs660339
AA
77
42
0.996
0.774
1.28
0.9781
AG
192
103
GG
98
53
rs659366
TT
80
45
0.972
0.757
1.247
0.968
TC
191
102
CC
96
51
rs649446
TT
38
19
1.003
0.739
1.362
0.699
TC
168
98
CC
161
81
rs586773
TT
83
47
0.959
0.749
1.226
0.9377
TA
185
98
AA
98
51
rs34408426
GG
83
47
0.959
0.749
1.226
0.9444
GA
184
99
AA
98
51
rs7109266
AA
36
18
0.997
0.729
1.363
0.631
AG
168
99
GG
163
81
rs3019463
TT
82
45
0.979
0.763
1.255
0.9793
TC
179
98
CC
97
51
rs591758
CC
83
47
0.959
0.749
1.226
0.9443
CG
186
100
GG
98
51
PPARγ
rs2920503
AA
34
23
0.891
0.665
1.193
0.438
AG
145
80
GG
177
95
rs73813168
GG
2
2
0.701
0.262
1.877
0.480
AA
285
140
AG
76
54
rs79310821
AA
44
23
1.108
0.834
1.471
0.480
AG
177
80
GG
145
93
rs73021485
TT
47
23
1.159
0.875
1.535
0.304
GG
143
94
GT
177
81
rs2920502
GG
25
25
0.715
0.527
0.97
0.031∗
CC
176
90
CG
164
82
rs17029007
AA
5
3
0.905
0.439
1.864
0.786
AG
71
54
GG
287
141
rs3856806
TT
16
3
1.58
1.104
2.761
0.032∗
CC
218
136
CT
131
58
∗P<0.05.


## 3.3. Haplotype Analysis

There was a linkage disequilibrium in PPARγ and UCP2, respectively. The haplotype frequency distribution of each gene between prediabetes/diabetes and normal glucose tolerance was summarized in Table 5; however, haplotype frequency was not significantly different between prediabetes/diabetes and control.Table 5
The haplotype frequency distribution between prediabetes/diabetes and normal glucose tolerance.
Gene name
Haplotype
Prediabetes/diabetes, normal glucose tolerance frequency
χ2
P value
UCP2
GCCAAGCG
0.511, 0.513
0.003
0.953
ATTTGATC
0.318, 0.329
0.133
0.715
ATCTGGTC
0.146, 0.143
0.021
0.884
PPARγ
CAATCG
0.362, 0.316
2.473
0.1158
TAGGCG
0.307, 0.318
0.149
0.6992
CAGGGG
0.182, 0.184
0.008
0.9304
CGGGGA
0.109, 0.146
3.268
0.0706
CAGGCG
0.029, 0.025
0.147
0.7015


## 3.4. Association of Genotype with Demographic Characteristics

In UCP2, the waist-to-hip ratio in subjects with AA genotype of rs7109266 were higher than that in subjects with GG or GA genotype, but age, BMI, and blood pressure were not different among genotypes of other SNPs (Table6). Age, BMI, blood pressure, and waist-to-hip ratio were not different among genotypes of selected SNPs in PPARγ (Table 7).Table 6
Association of genotype and demographic characteristics in UCP2.
Genotype
Age (year)
BMI (kg/m2)
Waist-to-hip ratio
Systolic blood pressure (mmHg)
Diastolic blood pressure (mmHg)
rs660339
GG
52.87 ± 1.03
25.62 ± 0.29
0.94 ± 0.00
129.51 ± 1.61
75.28 ± 0.78
AA
54.22 ± 1.00
25.94 ± 0.35
0.95 ± 0.02
128.74 ± 1.78
76.42 ± 0.96
GA
54.00 ± 0.66
26.07 ± 0.23
0.94 ± 0.00
126.78 ± 1.03
76.62 ± 0.59
P value
0.557
0.51
0.492
0.3
0.405
rs659366
CC
53.26 ± 1.03
25.55 ± 0.28
0.94 ± 0.00
129.69 ± 1.65
75.39 ± 0.79
TT
54.48 ± 1.00
25.91 ± 0.34
0.95 ± 0.02
128.60 ± 1.76
76.15 ± 0.93
TC
53.68 ± 0.67
26.11 ± 0.23
0.94 ± 0.00
126.75 ± 1.02
76.67 ± 0.59
P value
0.683
0.353
0.503
0.269
0.452
rs649446
CC
52.47 ± 0.78
25.63 ± 0.23
0.94 ± 0.00
128.65 ± 1.18
75.85 ± 0.64
TT
56.44 ± 1.51
26.71 ± 0.48
0.97 ± 0.04
128.98 ± 2.59
74.18 ± 1.41
TC
54.29 ± 0.68
26.02 ± 0.25
0.94 ± 0.00
126.87 ± 1.15
76.91 ± 0.61
P value
0.058
0.132
0.055
0.5
0.138
rs7109266
GG
52.61 ± 0.78
25.64 ± 0.23
0.93 ± 0.00
128.65 ± 1.17
75.78 ± 0.64
AA
56.28 ± 1.57
26.65 ± 0.50
0.98 ± 0.04
129.26 ± 2.70
74.69 ± 1.44
GA
54.24 ± 0.68
26.03 ± 0.25
0.94 ± 0.00
126.82 ± 1.14
76.84 ± 0.61
P value
0.068
0.177
0.017∗
0.456
0.254
rs591758
GG
53.24 ± 1.03
25.59 ± 0.28
0.94 ± 0.00
129.77 ± 1.63
75.56 ± 0.78
CC
54.71 ± 0.99
25.95 ± 0.34
0.95 ± 0.02
128.62 ± 1.76
76.05 ± 0.90
CG
53.57 ± 0.67
26.08 ± 0.24
0.94 ± 0.00
126.64 ± 1.03
76.64 ± 0.60
P value
0.539
0.45
0.43
0.224
0.558
rs586773
AA
53.24 ± 1.03
25.59 ± 0.28
0.94 ± 0.00
129.77 ± 1.63
75.56 ± 0.78
TT
54.71 ± 0.99
25.95 ± 0.34
0.95 ± 0.02
128.62 ± 1.76
76.05 ± 0.90
AT
53.64 ± 0.68
26.07 ± 0.24
0.94 ± 0.00
126.67 ± 1.03
76.66 ± 0.61
P value
0.553
0.459
0.436
0.235
0.548
rs34408426
AA
53.24 ± 1.03
25.59 ± 0.28
0.94 ± 0.00
129.77 ± 1.63
75.56 ± 0.78
GG
54.71 ± 0.99
25.95 ± 0.34
0.95 ± 0.02
128.62 ± 1.76
76.05 ± 0.90
AG
53.64 ± 0.67
26.06 ± 0.24
0.94 ± 0.00
126.77 ± 1.03
76.74 ± 0.60
P value
0.552
0.478
0.43
0.26
0.495
rs3019463
CC
53.24 ± 1.04
25.60 ± 0.28
0.94 ± 0.00
129.84 ± 1.64
75.53 ± 0.79
TT
54.71 ± 1.01
25.98 ± 0.34
0.95 ± 0.02
128.83 ± 1.79
76.04 ± 0.92
TC
53.58 ± 0.68
26.03 ± 0.24
0.94 ± 0.00
126.29 ± 1.03
76.38 ± 0.61
P value
0.551
0.519
0.429
0.138
0.709
∗P<0.05.Table 7
Association of genotype and demographic characteristics in PPARγ.
Genotype
Age (year)
BMI (kg/m2)
Waist-to-hip ratio
Systolic blood pressure (mmHg)
Diastolic blood pressure (mmHg)
rs2920503
CC
53.99 ± 0.71
26.09 ± 0.22
0.94 ± 0.01
128.06 ± 1.10
76.26 ± 0.57
CT
54.25 ± 0.74
25.69 ± 0.27
0.94 ± 0.00
128.02 ± 1.29
75.99 ± 0.71
TT
50.03 ± 1.54
25.78 ± 0.47
0.94 ± 0.00
126.33 ± 2.44
75.91 ± 1.45
P value
0.430
0.502
0.583
0.806
0.946
rs73813168
AA
53.79 ± 0.57
25.92 ± 0.19
0.94 ± 0.01
128.15 ± 0.87
76.04 ± 0.48
GA
53.41 ± 1.00
25.89 ± 0.32
0.94 ± 0.01
127.27 ± 1.82
77.02 ± 0.93
GG
52.25 ± 6.13
28.91 ± 1.87
0.94 ± 0.01
124.75 ± 15.30
69.00 ± 4.65
P value
0.92
0.29
0.995
0.845
0.215
rs79310821
GA
54.33 ± 0.67
25.73 ± 0.23
0.94 ± 0.00
127.97 ± 1.06
76.23 ± 0.61
GG
52.11 ± 0.77
26.19 ± 0.25
0.94 ± 0.00
127.57 ± 1.30
76.31 ± 0.69
AA
53.66 ± 1.55
25.77 ± 0.52
0.93 ± 0.01
128.56 ± 2.43
75.95 ± 1.11
P value
0.051
0.378
0.735
0.924
0.969
rs73021485
GT
55.29 ± 0.67
25.73 ± 0.23
0.95 ± 0.01
128.21 ± 1.06
76.30 ± 0.61
GG
53.87 ± 0.78
26.17 ± 0.25
0.94 ± 0.00
127.35 ± 1.30
76.17 ± 0.69
TT
54.29 ± 1.53
25.81 ± 0.50
0.93 ± 0.01
128.13 ± 2.38
75.75 ± 1.08
P value
0.058
0.425
0.577
0.867
0.923
rs2920502
GC
54.15 ± 0.71
25.93 ± 0.22
0.95 ± 0.01
128.24 ± 1.19
76.62 ± 0.64
CC
53.97 ± 0.72
25.77 ± 0.24
0.93 ± 0.00
127.89 ± 1.12
75.98 ± 0.61
GG
50.36 ± 1.85
26.72 ± 0.61
0.94 ± 0.00
126.33 ± 2.78
75.10 ± 1.43
P value
0.098
0.274
0.352
0.803
0.561
rs17029007
GG
53.76 ± 0.57
25.92 ± 0.19
0.94 ± 0.01
128.17 ± 0.86
76.07 ± 0.47
GA
53.74 ± 1.03
25.87 ± 0.34
0.94 ± 0.01
126.59 ± 1.86
77.01 ± 0.96
AA
50.75 ± 4.20
27.74 ± 1.04
0.94 ± 0.01
128.88 ± 10.20
68.75 ± 2.56
P value
0.768
0.399
0.992
0.698
0.069
rs3856806
CC
52.84 ± 0.62
25.84 ± 0.20
0.94 ± 0.00
128.17 ± 1.01
76.17 ± 0.53
TC
54.77 ± 0.84
26.03 ± 0.30
0.95 ± 0.01
127.02 ± 1.26
76.35 ± 0.73
TT
55.45 ± 2.47
26.33 ± 0.71
0.93 ± 0.01
130.75 ± 5.31
75.50 ± 2.73
P value
0.052
0.765
0.613
0.616
0.932


## 3.5. Association of Genotype with Insulin Secretion Function, Blood Glucose, and Lipid Profiles

Subjects with TT genotype of rs649446 or with AA genotype of rs7109266 in UCP2 had higher fasting insulin, HOMA-IR, and HOMA-β than subjects with other genotypes, but blood glucose profiles including fasting and 2 hr postprandial glucose were not significantly different among genotypes (Table 8). There was no significant difference in glucose profiles and insulin secretion in other loci of UCP2 (Table 8). The serum lipid TC, TG, HDL-C, and LDL-C were not significantly different among genotypes of selected SNPs in UCP2 (Table 9).Table 8
Association of genotype with insulin secretion function and blood glucose in UCP2.
Genotype
HbA1c%
FPG (mmol/l)
Glu30′ (mmol/l)
Glu60′ (mmol/l)
Glu120′ (mmol/l)
Ins0′ (μIU/ml)
Ins30′ (μIU/ml)
Ins60′ (μIU/ml)
Ins120′ (μIU/ml)
HOMA-IR
HOMA-β
DI30
DI120
rs660339
GG
6.06 ± 0.11
6.89 ± 0.21
11.37 ± 0.31
11.26 ± 0.42
9.66 ± 0.46
11.79 ± 0.77
78.32 ± 5.24
78.70 ± 4.76
51.97 ± 3.60
3.43 ± 0.23
88.43 ± 5.73
414.74 ± 23.47
536.01 ± 24.80
AA
5.99 ± 0.11
6.80 ± 0.23
11.23 ± 0.32
11.38 ± 0.44
9.55 ± 0.48
12.48 ± 1.16
69.12 ± 4.91
74.85 ± 4.53
57.01 ± 3.97
3.92 ± 0.43
90.93 ± 6.62
383.99 ± 27.42
514.93 ± 28.03
GA
6.01 ± 0.07
6.68 ± 0.13
11.01 ± 0.21
10.92 ± 0.29
8.83 ± 0.28
12.29 ± 0.61
71.72 ± 3.28
78.81 ± 3.47
58.45 ± 3.07
3.97 ± 0.33
89.23 ± 3.38
420.51 ± 17.56
587.96 ± 34.53
P value
0.894
0.677
0.592
0.633
0.187
0.849
0.379
0.803
0.412
0.524
0.95
0.518
0.294
rs659366
CC
6.05 ± 0.11
6.88 ± 0.22
11.37 ± 0.32
11.28 ± 0.43
9.68 ± 0.47
11.80 ± 0.79
77.38 ± 5.27
78.68 ± 4.82
52.52 ± 3.68
3.43 ± 0.24
88.80 ± 5.86
410.35 ± 23.43
535.63 ± 25.18
TT
5.93 ± 0.11
6.74 ± 0.22
11.09 ± 0.31
11.07 ± 0.43
9.31 ± 0.46
12.56 ± 1.12
71.34 ± 5.05
75.55 ± 4.77
58.35 ± 4.31
3.88 ± 0.42
92.55 ± 6.54
395.63 ± 27.34
526.68 ± 27.42
TC
6.04 ± 0.07
6.71 ± 0.14
11.07 ± 0.21
11.04 ± 0.29
8.92 ± 0.28
12.24 ± 0.60
71.39 ± 3.25
78.60 ± 3.41
57.57 ± 2.98
3.99 ± 0.33
88.32 ± 3.34
418.18 ± 17.59
583.49 ± 34.57
P value
0.677
0.796
0.706
0.889
0.327
0.84
0.56
0.871
0.526
0.518
0.821
0.777
0.43
rs649446
CC
6.06 ± 0.09
6.84 ± 0.16
11.24 ± 0.24
11.20 ± 0.33
9.46 ± 0.34
12.05 ± 0.55
75.14 ± 3.99
79.96 ± 3.95
56.72 ± 3.32
3.61 ± 0.18
90.91 ± 4.38
404.52 ± 18.16
535.54 ± 20.23
TT
6.06 ± 0.16
6.90 ± 0.32
11.50 ± 0.47
11.65 ± 0.65
10.06 ± 0.73
16.71 ± 2.96
74.87 ± 7.74
83.49 ± 7.86
67.33 ± 7.15
5.78 ± 1.53
108.66 ± 12.90
335.15 ± 26.95
466.10 ± 31.42
TC
5.98 ± 0.07
6.67 ± 0.15
11.01 ± 0.21
10.93 ± 0.29
8.81 ± 0.30
11.38 ± 0.50
70.59 ± 3.32
75.06 ± 3.24
54.06 ± 2.76
3.59 ± 0.23
83.77 ± 3.10
433.37 ± 19.96
599.24 ± 37.91
P value
0.765
0.676
0.581
0.567
0.156
0.003∗
0.655
0.469
0.176
0.006∗
0.026∗
0.066
0.096
rs7109266
GG
6.07 ± 0.09
6.86 ± 0.16
11.24 ± 0.24
11.24 ± 0.33
9.48 ± 0.34
12.03 ± 0.55
74.80 ± 3.96
79.68 ± 3.92
56.77 ± 3.29
3.62 ± 0.18
90.40 ± 4.35
402.30 ± 18.07
533.33 ± 20.14
AA
6.07 ± 0.17
6.96 ± 0.33
11.58 ± 0.50
11.79 ± 0.67
10.16 ± 0.77
17.11 ± 3.12
76.19 ± 8.10
84.59 ± 8.20
68.44 ± 7.51
5.98 ± 1.61
109.45 ± 13.52
334.04 ± 28.21
461.60 ± 33.00
GA
5.97 ± 0.07
6.64 ± 0.15
10.99 ± 0.21
10.87 ± 0.29
8.78 ± 0.30
11.37 ± 0.49
70.64 ± 3.31
75.15 ± 3.23
53.93 ± 2.75
3.57 ± 0.23
84.30 ± 3.09
434.69 ± 19.84
601.13 ± 37.71
P value
0.601
0.502
0.49
0.401
0.107
0.001∗
0.656
0.449
0.137
0.003∗
0.031∗
0.06
0.078
rs591758
GG
6.05 ± 0.11
6.87 ± 0.21
11.33 ± 0.31
11.24 ± 0.42
9.66 ± 0.46
11.91 ± 0.79
77.66 ± 5.22
78.40 ± 4.75
52.65 ± 3.63
3.46 ± 0.23
89.39 ± 5.7
410.86 ± 23.20
534.33 ± 24.89
CC
5.94 ± 0.11
6.78 ± 0.23
11.11 ± 0.30
11.11 ± 0.41
9.32 ± 0.45
13.43 ± 1.36
73.30 ± 5.06
78.32 ± 4.83
59.57 ± 4.33
4.45 ± 0.70
94.35 ± 6.42
392.34 ± 26.39
525.85 ± 26.71
CG
6.04 ± 0.07
6.70 ± 0.14
11.07 ± 0.21
11.04 ± 0.29
8.91 ± 0.29
11.79 ± 0.48
70.32 ± 3.25
77.56 ± 3.43
56.98 ± 3.00
3.71 ± 0.21
87.12 ± 3.36
419.88 ± 17.95
585.93 ± 35.37
P value
0.73
0.775
0.77
0.92
0.343
0.322
0.457
0.987
0.485
0.209
0.57
0.682
0.381
rs586773
AA
6.05 ± 0.11
6.87 ± 0.21
11.33 ± 0.31
11.24 ± 0.42
9.66 ± 0.46
11.91 ± 0.79
77.66 ± 5.22
78.40 ± 4.75
52.65 ± 3.63
3.46 ± 0.23
89.39 ± 5.79
410.86 ± 23.20
534.33 ± 24.89
TT
5.94 ± 0.11
6.78 ± 0.23
11.11 ± 0.30
11.11 ± 0.41
9.32 ± 0.45
13.43 ± 1.36
73.30 ± 5.06
78.32 ± 4.83
59.57 ± 4.33
4.45 ± 0.70
94.35 ± 6.42
392.34 ± 26.39
525.85 ± 26.71
AT
6.04 ± 0.07
6.71 ± 0.14
11.10 ± 0.21
11.09 ± 0.30
8.93 ± 0.29
11.80 ± 0.49
70.25 ± 3.29
77.60 ± 3.46
57.04 ± 3.03
3.73 ± 0.22
86.95 ± 3.39
418.26 ± 18.06
583.80 ± 35.73
P value
0.711
0.804
0.798
0.952
0.365
0.33
0.453
0.988
0.484
0.214
0.557
0.713
0.413
rs34408426
AA
6.05 ± 0.11
6.87 ± 0.21
11.33 ± 0.31
11.24 ± 0.42
9.66 ± 0.46
11.91 ± 0.79
77.66 ± 5.22
78.40 ± 4.75
52.65 ± 3.63
3.46 ± 0.23
89.39 ± 5.79
410.86 ± 23.20
534.33 ± 24.89
GG
5.94 ± 0.11
6.78 ± 0.23
11.11 ± 0.30
11.11 ± 0.41
9.32 ± 0.45
13.43 ± 1.36
73.30 ± 5.06
78.32 ± 4.83
59.57 ± 4.33
4.45 ± 0.70
94.35 ± 6.42
392.34 ± 26.39
525.85 ± 26.71
AG
6.03 ± 0.07
6.67 ± 0.13
11.02 ± 0.21
11.00 ± 0.29
8.90 ± 0.29
11.81 ± 0.49
70.71 ± 3.28
77.99 ± 3.46
57.37 ± 3.02
3.71 ± 0.21
87.48 ± 3.38
421.79 ± 18.08
588.64 ± 35.70
P value
0.747
0.698
0.694
0.882
0.331
0.332
0.499
0.997
0.471
0.209
0.602
0.646
0.35
rs3019463
CC
6.03 ± 0.11
6.85 ± 0.21
11.30 ± 0.31
11.19 ± 0.42
9.60 ± 0.46
11.91 ± 0.79
77.96 ± 5.25
78.59 ± 4.78
52.63 ± 3.66
3.45 ± 0.24
89.77 ± 5.82
413.23 ± 23.25
537.30 ± 24.89
TT
5.96 ± 0.11
6.82 ± 0.23
11.15 ± 0.31
11.23 ± 0.42
9.40 ± 0.46
13.54 ± 1.39
72.78 ± 5.11
79.20 ± 4.90
60.35 ± 4.41
4.51 ± 0.72
94.20 ± 6.53
381.46 ± 25.95
518.95 ± 26.89
TC
6.02 ± 0.07
6.67 ± 0.14
11.08 ± 0.22
11.00 ± 0.30
8.91 ± 0.29
11.75 ± 0.50
70.74 ± 3.30
78.34 ± 3.50
57.65 ± 3.08
3.70 ± 0.22
87.13 ± 3.38
425.19 ± 18.44
593.35 ± 36.28
P value
0.864
0.74
0.847
0.883
0.369
0.278
0.473
0.99
0.41
0.172
0.59
0.39
0.275
∗P<0.05. DI30 (early-phase disposition index of insulin secretion) = [InsAUC30/GluAUC30] × ISIM, DI120 (total-phase disposition of insulin secretion) = [InsAUC120/GluAUC120] × ISIM.Table 9
Association of genotype with lipid profiles in UCP2.
Genotype
TC (mmol/l)
TG (mmol/l)
HDL-C (mmol/l)
LDL-C (mmol/l)
TG/HDL-C
rs660339
GG
5.44 ± 0.09
1.81 ± 0.16
1.35 ± 0.04
2.82 ± 0.06
1.45 ± 0.12
AA
5.49 ± 0.10
1.72 ± 0.10
1.30 ± 0.03
2.85 ± 0.07
1.45 ± 0.10
GA
5.39 ± 0.06
2.16 ± 0.41
1.29 ± 0.02
2.83 ± 0.04
1.79 ± 0.32
P value
0.702
0.678
0.359
0.93
0.627
rs659366
CC
5.44 ± 0.09
1.73 ± 0.14
1.34 ± 0.05
2.83 ± 0.06
1.41 ± 0.11
TT
5.49 ± 0.10
1.69 ± 0.10
1.30 ± 0.03
2.86 ± 0.07
1.43 ± 0.09
TC
5.39 ± 0.06
2.22 ± 0.42
1.29 ± 0.02
2.82 ± 0.04
1.82 ± 0.32
P value
0.672
0.536
0.405
0.919
0.519
rs649446
CC
5.35 ± 0.07
2.34 ± 0.53
1.34 ± 0.03
2.77 ± 0.05
1.85 ± 0.40
TT
5.61 ± 0.16
1.66 ± 0.12
1.29 ± 0.04
2.95 ± 0.12
1.40 ± 0.12
TC
5.44 ± 0.06
1.72 ± 0.07
1.28 ± 0.02
2.86 ± 0.05
1.48 ± 0.07
P value
0.254
0.39
0.257
0.157
0.544
rs7109266
GG
5.35 ± 0.07
2.33 ± 0.52
1.34 ± 0.03
2.77 ± 0.05
1.84 ± 0.40
AA
5.56 ± 0.17
1.63 ± 0.12
1.28 ± 0.04
2.93 ± 0.12
1.39 ± 0.13
GA
5.45 ± 0.06
1.73 ± 0.07
1.28 ± 0.02
2.87 ± 0.04
1.48 ± 0.07
P value
0.351
0.404
0.249
0.206
0.563
rs591758
GG
5.44 ± 0.09
1.72 ± 0.13
1.34 ± 0.04
2.84 ± 0.06
1.41 ± 0.11
CC
5.49 ± 0.10
1.69 ± 0.10
1.30 ± 0.03
2.86 ± 0.07
1.43 ± 0.09
CG
5.38 ± 0.06
2.23 ± 0.43
1.29 ± 0.02
2.82 ± 0.04
1.83 ± 0.33
P value
0.633
0.509
0.497
0.846
0.501
rs586773
AA
5.44 ± 0.09
1.72 ± 0.13
1.34 ± 0.04
2.84 ± 0.06
1.41 ± 0.11
TT
5.49 ± 0.10
1.69 ± 0.10
1.30 ± 0.03
2.86 ± 0.07
1.43 ± 0.09
AT
5.38 ± 0.06
2.23 ± 0.43
1.30 ± 0.02
2.82 ± 0.04
1.82 ± 0.33
P value
0.635
0.515
0.515
0.855
0.508
rs34408426
AA
5.44 ± 0.09
1.72 ± 0.13
1.34 ± 0.04
2.84 ± 0.06
1.41 ± 0.11
GG
5.49 ± 0.10
1.69 ± 0.10
1.30 ± 0.03
2.86 ± 0.07
1.43 ± 0.09
AG
5.37 ± 0.06
2.20 ± 0.43
1.29 ± 0.02
2.81 ± 0.04
1.82 ± 0.33
P value
0.536
0.554
0.449
0.829
0.521
rs3019463
CC
5.43 ± 0.09
1.72 ± 0.14
1.34 ± 0.05
2.83 ± 0.06
1.41 ± 0.11
TT
5.51 ± 0.10
1.69 ± 0.10
1.30 ± 0.03
2.87 ± 0.07
1.43 ± 0.09
TC
5.38 ± 0.07
2.23 ± 0.44
1.30 ± 0.02
2.81 ± 0.04
1.82 ± 0.34
P value
0.538
0.522
0.561
0.744
0.527Subjects with GG genotype of rs2920502 in PPARγ had better HbA1c, 0 min, 30 min, and 120 min blood glucose, increased 60 min and 120 min insulin secretion after taking 75 g glucose, and lower serum TC, TG, and LDL-C compared to GC/CC genotypes (Table 10); the HOMA-IR in GG genotype was lower than GC/CC genotypes. Subjects with TT genotype of rs2920503 in PPARγ had better HbA1c, 0 min, 30 min, 60 min, and 120 min blood glucose and had increased serum insulin in 120 min after taking 75 g glucose compared to TC/CC genotypes (Table 10). Subjects with TT genotypes of rs3856806 had higher fasting blood glucose than TC/CC genotypes, and postprandial blood glucose and insulin secretion were not significantly different among genotypes. The blood glucose at 0 min, 30 min, 60 min, and 120 min after taking 75 g glucose in subjects with AA/GG genotype of rs79310821 were better than subjects with GA genotype. The blood glucose at 0 min, 30 min, 60 min, and 120 min after taking 75 g glucose in subjects with TT/GG genotype of rs79310821 was better than that in subjects with TG genotype, and index of insulin secretion-HOMA-β, DI30, and DI120 were higher in TT/GG genotype than in TG genotype. The serum lipid profiles were not significantly different in other loci in PPARγ (Table 11).Table 10
Association of genotype with insulin secretion function and blood glucose in PPARγ.
Genotype
HbA1c%
FPG (mmol/l)
Glu30′ (mmol/l)
Glu60′ (mmol/l)
Glu120′ (mmol/l)
Ins0′ (μIU/ml)
Ins30′ (μIU/ml)
Ins60′ (μIU/ml)
Ins120′ (μIU/ml)
HOMA-IR
HOMA-β
DI30
DI120
rs2920503
CC
6.06 ± 0.08
6.84 ± 0.16
11.34 ± 0.23
11.16 ± 0.32
9.17 ± 0.33
11.59 ± 0.57
73.21 ± 3.49
74.50 ± 3.44
50.88 ± 2.74
3.69 ± 0.33
85.26 ± 3.25
423.09 ± 18.98
577.78 ± 37.56
CT
6.10 ± 0.08
6.87 ± 0.16
11.32 ± 0.25
11.55 ± 0.33
9.69 ± 0.35
13.10 ± 0.86
72.05 ± 3.91
82.18 ± 3.95
53.29 ± 6.52
4.15 ± 0.32
92.27 ± 5.27
387.24 ± 19.31
527.33 ± 20.78
TT
5.59 ± 0.11
5.98 ± 0.12
9.82 ± 0.29
9.33 ± 0.44
7.72 ± 0.35
12.05 ± 0.92
78.26 ± 8.06
78.79 ± 6.95
62.76 ± 3.49
3.31 ± 0.29
101.01 ± 6.49
460.64 ± 35.70
592.80 ± 31.89
P value
0.019∗
0.039∗
0.012∗
0.01∗
0.034∗
0.295
0.77
0.332
0.024∗
0.405
0.186
0.178
0.434
rs73813168
AA
6.02 ± 0.06
6.77 ± 0.12
11.16 ± 0.18
11.14 ± 0.24
9.32 ± 0.25
11.90 ± 0.41
71.68 ± 2.77
78.64 ± 2.76
57.65 ± 2.39
3.67 ± 0.17
87.72 ± 2.87
402.67 ± 13.84
556.93 ± 24.92
GA
6.04 ± 0.11
6.78 ± 0.23
11.21 ± 0.32
11.13 ± 0.46
8.87 ± 0.46
12.14 ± 1.04
75.88 ± 5.27
76.82 ± 5.22
52.35 ± 4.13
3.99 ± 0.63
89.11 ± 5.46
439.29 ± 29.79
570.00 ± 31.05
GG
5.85 ± 0.12
5.99 ± 0.19
11.09 ± 0.80
10.54 ± 1.91
6.21 ± 1.12
12.59 ± 3.34
99.09 ± 51.54
73.55 ± 20.46
43.04 ± 15.77
3.35 ± 0.86
102.59 ± 30.85
414.77 ± 154.51
513.87 ± 66.05
P value
0.947
0.814
0.99
0.97
0.336
0.96
0.506
0.939
0.479
0.777
0.864
0.477
0.946
rs79310821
GA
6.16 ± 0.08
7.08 ± 0.18
11.59 ± 0.24
11.76 ± 0.33
9.92 ± 0.35
12.81 ± 0.87
68.22 ± 3.24
76.64 ± 3.47
56.29 ± 3.03
3.92 ± 0.36
83.64 ± 3.79
412.36 ± 20.56
522.64 ± 21.28
GG
5.91 ± 0.08
6.52 ± 0.13
10.80 ± 0.22
10.68 ± 0.31
8.71 ± 0.31
12.14 ± 0.48
76.31 ± 3.96
78.00 ± 3.63
56.77 ± 3.09
3.80 ± 0.28
91.79 ± 4.26
403.67 ± 16.44
586.01 ± 36.88
AA
5.89 ± 0.11
6.39 ± 0.17
10.67 ± 0.37
10.13 ± 0.51
8.30 ± 0.52
10.14 ± 0.62
78.61 ± 8.39
80.96 ± 8.19
56.84 ± 6.70
3.50 ± 0.33
105.43 ± 8.54
442.89 ± 46.25
583.89 ± 43.95
P value
0.055
0.015∗
0.029∗
0.012∗
0.009∗
0.192
0.21
0.856
0.993
0.855
0.069
0.695
0.301
rs73021485
GT
6.14 ± 0.08
7.06 ± 0.18
11.59 ± 0.24
11.74 ± 0.32
9.86 ± 0.34
12.32 ± 0.82
68.11 ± 3.22
76.83 ± 3.47
56.48 ± 3.03
4.14 ± 0.40
82.09 ± 3.90
373.32 ± 17.21
509.27 ± 19.15
GG
5.91 ± 0.08
6.51 ± 0.13
10.80 ± 0.22
10.67 ± 0.31
8.67 ± 0.31
12.17 ± 0.48
76.73 ± 3.97
78.64 ± 3.66
56.42 ± 3.09
3.56 ± 0.17
96.67 ± 4.06
438.29 ± 20.28
564.71 ± 19.91
TT
5.97 ± 0.12
6.51 ± 0.19
10.74 ± 0.39
10.30 ± 0.55
8.59 ± 0.57
11.87 ± 1.25
78.18 ± 8.14
80.13 ± 7.86
56.92 ± 6.44
3.52 ± 0.48
91.36 ± 9.05
460.55 ± 37.27
724.86 ± 133.17I
P value
0.112
0.028∗
0.031∗
0.019∗
0.021∗
0.949
0.186
0.89
0.997
0.363
0.041∗
0.018∗
0.004∗
rs2920502
GC
6.20 ± 0.10
7.11 ± 0.19
11.58 ± 0.26
11.67 ± 0.35
8.98 ± 0.28
11.75 ± 0.64
71.26 ± 3.88
73.99 ± 3.59
54.91 ± 5.56
3.56 ± 0.17
84.54 ± 4.31
436.29 ± 20.24
561.96 ± 19.83
CC
6.02 ± 0.06
7.15 ± 0.11
11.85 ± 0.19
11.76 ± 0.27
9.76 ± 0.38
12.55 ± 0.73
73.30 ± 3.47
70.05 ± 3.57
50.38 ± 2.98
4.33 ± 0.42
96.17 ± 4.05
373.56 ± 17.36
507.65 ± 19.37
GG
5.74 ± 0.11
6.25 ± 0.24
10.72 ± 0.46
10.43 ± 0.64
7.82 ± 0.52
12.68 ± 1.00
80.64 ± 7.31
85.24 ± 7.56
62.44 ± 3.16
2.81 ± 0.19
83.97 ± 6.40
470.32 ± 37.73
743.78 ± 136.80
P value
0.009∗
0.01∗
0.049∗
0.068
0.032∗
0.663
0.579
0.015∗
0.019∗
0.041∗
0.104
0.015∗
0.002∗
rs17029007
GG
6.01 ± 0.06
6.77 ± 0.12
11.14 ± 0.18
11.12 ± 0.24
9.30 ± 0.25
12.18 ± 0.50
71.74 ± 2.75
78.71 ± 2.74
57.73 ± 2.40
3.76 ± 0.19
89.10 ± 3.15
402.92 ± 13.76
557.25 ± 24.78
GA
6.00 ± 0.11
6.76 ± 0.24
11.23 ± 0.33
11.16 ± 0.48
8.98 ± 0.49
12.35 ± 1.09
75.56 ± 5.32
76.13 ± 5.39
52.52 ± 4.26
4.05 ± 0.65
90.98 ± 5.74
437.31 ± 30.27
563.96 ± 31.32
AA
6.26 ± 0.50
6.01 ± 0.16
10.68 ± 0.62
9.78 ± 1.21
6.35 ± 0.79
10.79 ± 1.81
111.10 ± 30.97
79.93 ± 13.81
49.28 ± 10.87
2.87 ± 0.47
89.40 ± 17.08
514.60 ± 108.50
642.35 ± 83.52
P value
0.837
0.684
0.903
0.741
0.231
0.92
0.138
0.904
0.532
0.725
0.96
0.319
0.873
rs3856806
CC
5.96 ± 0.06
6.57 ± 0.11
10.92 ± 0.18
10.78 ± 0.25
8.93 ± 0.26
11.87 ± 0.41
75.61 ± 3.14
77.37 ± 3.07
56.12 ± 2.58
3.51 ± 0.14
91.40 ± 3.05
432.97 ± 16.46
582.14 ± 29.37
TC
6.12 ± 0.10
7.11 ± 0.22
11.58 ± 0.29
11.68 ± 0.39
9.69 ± 0.40
12.75 ± 1.08
69.35 ± 4.21
78.76 ± 4.16
56.36 ± 3.54
4.33 ± 0.53
86.40 ± 5.67
377.68 ± 20.08
518.92 ± 22.77
TT
6.08 ± 0.21
7.88 ± 0.47
11.33 ± 0.82
11.60 ± 1.24
9.27 ± 1.42
12.59 ± 2.01
62.15 ± 8.81
77.54 ± 11.07
58.24 ± 12.15
4.36 ± 1.22
82.71 ± 11.35
358.63 ± 57.56
506.31 ± 62.29
P value
0.326
0.047∗
0.127
0.121
0.257
0.65
0.338
0.964
0.981
0.155
0.619
0.085
0.302
∗P<0.05. DI30 (early-phase disposition index of insulin secretion) = [InsAUC30/GluAUC30] × ISIM, DI120 (total-phase disposition of insulin secretion) = [InsAUC120/GluAUC120] × ISIM.Table 11
Association of genotype with lipid profiles in PPARγ.
Genotype
TC (mmol/l)
TG (mmol/l)
HDL-C (mmol/l)
LDL-C (mmol/l)
TG/HDL-C
rs2920503
CC
5.51 ± 0.07
2.25 ± 0.46
1.32 ± 0.03
2.87 ± 0.05
1.81 ± 0.35
CT
5.36 ± 0.08
1.72 ± 0.10
1.28 ± 0.02
2.83 ± 0.05
1.44 ± 0.08
TT
5.26 ± 0.11
1.78 ± 0.16
1.33 ± 0.08
2.66 ± 0.08
1.56 ± 0.17
P value
0.154
0.53
0.457
0.146
0.594
rs73813168
AA
5.37 ± 0.05
1.78 ± 0.08
1.29 ± 0.02
2.80 ± 0.03
1.49 ± 0.06
GA
5.44 ± 0.11
2.67 ± 0.94
1.37 ± 0.05
2.97 ± 0.08
2.11 ± 0.72
GG
5.26 ± 0.59
1.36 ± 0.23
1.27 ± 0.13
2.73 ± 0.44
1.06 ± 0.12
P value
0.054
0.245
0.164
0.057
0.313
rs79310821
GA
5.49 ± 0.06
1.70 ± 0.08
1.30 ± 0.02
2.88 ± 0.04
1.41 ± 0.07
GG
5.42 ± 0.08
1.81 ± 0.11
1.33 ± 0.03
2.83 ± 0.05
1.49 ± 0.09
AA
5.20 ± 0.12
1.88 ± 0.24
1.25 ± 0.03
2.67 ± 0.09
1.61 ± 0.18
P value
0.147
0.629
0.283
0.109
0.484
rs73021485
GT
5.49 ± 0.06
1.70 ± 0.08
1.29 ± 0.02
2.88 ± 0.04
1.41 ± 0.07
GG
5.41 ± 0.08
2.30 ± 0.52
1.33 ± 0.03
2.83 ± 0.05
1.87 ± 0.40
TT
5.19 ± 0.12
1.85 ± 0.23
1.26 ± 0.03
2.67 ± 0.09
1.58 ± 0.18
P value
0.144
0.452
0.313
0.109
0.464
rs2920502
GC
5.48 ± 0.07
2.39 ± 0.08
1.31 ± 0.02
2.90 ± 0.05
1.82 ± 0.08
CC
5.72 ± 0.20
4.35 ± 2.43
1.28 ± 0.02
2.98 ± 0.15
3.40 ± 0.07
GG
5.32 ± 0.06
1.73 ± 0.11
1.42 ± 0.12
2.75 ± 0.04
1.22 ± 0.26
P value
0.034∗
0.004∗∗
0.07
0.031∗
0.006∗∗
rs17029007
GG
5.37 ± 0.05
1.78 ± 0.07
1.29 ± 0.02
2.80 ± 0.03
1.49 ± 0.06
GA
5.58 ± 0.10
2.70 ± 0.98
1.38 ± 0.05
2.91 ± 0.07
2.13 ± 0.75
AA
5.55 ± 0.45
1.46 ± 0.23
1.29 ± 0.09
2.94 ± 0.31
1.15 ± 0.17
P value
0.165
0.233
0.071
0.334
0.301
rs3856806
CC
5.42 ± 0.06
2.04 ± 0.35
1.30 ± 0.02
2.84 ± 0.04
1.71 ± 0.27
TC
5.44 ± 0.08
1.91 ± 0.16
1.32 ± 0.02
2.81 ± 0.05
1.52 ± 0.11
TT
5.39 ± 0.27
1.48 ± 0.17
1.25 ± 0.06
2.88 ± 0.16
1.27 ± 0.16
P value
0.971
0.882
0.741
0.846
0.811
∗P<0.05 and ∗∗P<0.01.


## 3.6. Association of Genotype with Inflammation

There was no significant difference in TNF-α among genotypes in UCP2. The serum IL-6 was higher in subjects with TT genotype of rs660339 than in GG/GA genotype, and IL-6 was higher in subjects with TT genotype of rs649446 than in CC/TC genotype (Table 12). There was no significant difference in inflammation indicators among genotypes in PPARγ (Table 13).Table 12
Association of genotype with inflammation in UCP2.
Genotype
TNF-α (fmol/ml)
IL-6 (pg/ml)
rs660339
GG
23.44 ± 0.86
1.62 ± 0.08
AA
22.25 ± 0.95
1.42 ± 0.10
GA
22.40 ± 0.57
1.70 ± 0.05
P value
0.527
0.034∗
rs659366
CC
23.31 ± 0.87
1.64 ± 0.08
TT
22.10 ± 0.94
1.46 ± 0.10
TC
22.55 ± 0.57
1.67 ± 0.05
P value
0.598
0.111
rs649446
CC
23.28 ± 0.66
1.68 ± 0.06
TT
20.97 ± 1.48
1.95 ± 0.16
TC
22.37 ± 0.59
1.65 ± 0.06
P value
0.257
0.001∗∗
rs7109266
GG
23.22 ± 0.66
1.68 ± 0.06
AA
20.78 ± 1.50
1.52 ± 0.17
GA
22.45 ± 0.60
1.65 ± 0.06
P value
0.257
0.063
rs591758
GG
23.37 ± 0.86
1.64 ± 0.08
CC
21.81 ± 0.89
1.46 ± 0.10
CG
22.65 ± 0.59
1.68 ± 0.06
P value
0.439
0.111
rs586773
AA
23.37 ± 0.86
1.64 ± 0.08
TT
21.81 ± 0.89
1.46 ± 0.10
AT
22.61 ± 0.59
1.67 ± 0.06
P value
0.437
0.12
rs34408426
AA
23.37 ± 0.86
1.64 ± 0.08
GG
21.81 ± 0.89
1.46 ± 0.10
AG
22.56 ± 0.59
1.67 ± 0.06
P value
0.434
0.124
rs3019463
CC
23.34 ± 0.86
1.64 ± 0.08
TT
22.15 ± 0.89
1.47 ± 0.10
TC
22.58 ± 0.60
1.66 ± 0.06
P value
0.606
0.17
∗P<0.05 and ∗∗P<0.01. TNF-α: tumor necrosis factor-α; IL-6: interleukine-6. IL-6 has been nature logarithm transformed.Table 13
Association of genotype with inflammation in PPARγ.
Genotype
TNF-α (fmol/ml)
IL-6 (pg/ml)
rs2920503
CC
22.60 ± 0.64
57.77 ± 0.96
CT
22.42 ± 0.62
60.82 ± 1.02
TT
23.51 ± 1.51
60.76 ± 2.40
P value
0.778
0.081
rs73813168
AA
23.11 ± 0.49
1.63 ± 0.05
GA
21.68 ± 0.88
1.56 ± 0.09
GG
16.44 ± 4.27
1.76 ± 0.27
P value
0.164
0.742
rs79310821
GA
23.02 ± 0.63
1.64 ± 0.06
GG
22.34 ± 0.66
1.59 ± 0.07
AA
22.29 ± 1.25
1.64 ± 0.11
P value
0.723
0.844
rs73021485
GT
22.99 ± 0.63
1.64 ± 0.06
GG
22.32 ± 0.67
1.59 ± 0.07
TT
22.28 ± 1.22
1.60 ± 0.11
P value
0.735
0.81
rs2920502
GC
22.78 ± 0.67
1.57 ± 0.06
CC
22.70 ± 0.61
1.68 ± 0.06
GG
21.41 ± 1.41
1.45 ± 0.14
P value
0.665
0.231
rs17029007
GG
23.03 ± 0.49
1.63 ± 0.05
GA
21.70 ± 0.87
1.55 ± 0.10
AA
17.39 ± 3.82
1.80 ± 0.22
P value
0.137
0.634
rs3856806
CC
22.62 ± 0.52
1.58 ± 0.05
TC
22.63 ± 0.79
1.69 ± 0.07
TT
21.87 ± 2.18
1.58 ± 0.24
P value
0.949
0.419
TNF-α: tumor necrosis factor-α; IL-6: interleukine-6. IL-6 has been nature logarithm transformed.


## 4. Discussion

The effects of UCP2 on proton leakage and the decline in ATP synthesis inβ-cells show that this protein is a negative regulator of insulin secretion. Increased expression of UCP2 results in decreased ATP synthesis, which inhibits ATP-sensitive potassium (K-ATP) channels, leading to the decline of glucose-stimulated insulin secretion [1]. Our previous study showed that UCP2 deficiency led to the amelioration of lipid metabolism and improved blood glucose by simultaneously promoting insulin sensitivity and β-cell function [1, 2]. Obesity and T2DM closely associated with SNPs in UCP2, including rs660339 (Ala55Val), rs659366 (-866G/A), and rs591758 [7]. In this study based on Chinese Han population in Beijing district, we selected 8 SNPs in the functional region of UCP2, and the results indicated that the alleles and genotypes were not significantly different between prediabetes/diabetes and control. Further genotype and clinical features analysis showed that subjects with TT genotype of rs649446 or subjects with AA genotype of rs7109266 in UCP2 had higher HOMA-IR and HOMA-β, subjects with AA genotype of rs7109266 also had higher waist-to-hip ratio, which suggested that subjects with TT genotype of rs649446 or subjects with AA genotype of rs7109266 were more susceptible to develop insulin resistance. Previous study showed that human islets with GA genotype of UCP2-866 polymorphism have decreased glucose-stimulated insulin secretion compared to GG genotype islets [3]. However, the pathway between UCP2 polymorphism and HOMA index has not been elaborated clearly. The study was the first one to investigate the association of the above SNPs with insulin resistance in Chinese Han population in Beijing district, it probably could give certain suggestion to prevent insulin resistance in early stage by classifying the genotype of the above SNPs inUCP2.The inflammation pathway is involved in the pathophysiology of diabetes and obesity. The study indicated that subjects with GG/GA genotype of rs660339 in UCP2 had higher serum IL-6 levels than those with AA genotype, and subjects with TT genotype of rs649446 had higher IL-6 than those with CC/TC genotypes. IL-6 is a central player in the regulation of inflammation, leading to insulin resistance. Its quantitative release from adipose tissue results in a subclinical and systemic elevation of IL-6 plasma levels with increasing body fat content, which may be implicated in the proinflammatory state leading to insulin resistance [20]. On the other hand, IL-6 produced in the working muscle during physical activity could act as an energy sensor by activating AMP-activated kinase and enhancing glucose disposal, lipolysis, and fat oxidation. In addition, both impaired IL-6 secretion and action are risk factors for weight gain [21]. Previous study suggested that people with GG/GA genotype of rs660339 in UCP2 had an increased risk for diabetes, obesity, and metabolic syndrome; the elevated IL-6 in the subjects with GG/GA genotype suggested that these kinds of SNP was closely related to inflammation, which play an important role in the mechanism of diabetes and its complications.PPARγ, which is a central nuclear receptor, is involved in fatty acid and glucose metabolism and is closely associated with insulin sensitivity. In clinical work, PPARγ agonist glitazone—the derivative of thiazolidinediones—could improve insulin resistance by indirectly increasing insulin-stimulated glucose uptake in adipocytes, skeletal muscle cells, and hepatocytes and inhibiting proinflammation cytokines produced from mononuclear macrophages [22]. Our previous study showed that UCP2 deficiency could improve insulin sensitivity and β-cell function by PPAR signaling pathway. PPARγ regulates UCP2 in the condition of a high-fat diet [14]. Among the selected 7 SNPs of PPARγ in our study, two loci (rs2920502 and rs3856806) were reported to be related to glucolipid metabolism [22]. This study suggested that subjects with GG genotype of rs2920502 in PPARγ, who had better early- and total-stage insulin secretion function and better serum lipid condition, had a decreased risk for diabetes in Chinese Han population of Beijing district. Prakash et al. reported that in Nanjing and Southwest district of China, GG genotype of rs2920502 was a protective factor for metabolism syndrome, GG carriers had elevated serum adiponectin, which is a kind of anti-inflammatory and antiatherosclerosis cytokine that could prevent metabolism syndrome; therefore, GG genotype of rs2920502 probably improved glucolipid metabolism by regulating the secretion of adiponectin [22]. In our study, subjects with TT genotype of rs3856806 in PPARγ had an increased risk for diabetes, and the result was in accordance with a previous study based on Chinese Han population; however, studies based on Indians and Singaporeans showed that TT genotype of rs3856806 could decrease the risk for diabetes. Evidence also showed that rs3856806 in PPARγ had a close relationship with metabolic syndrome, subjects with TT genotype had higher BMI in males, and those with TT/TC genotypes had higher systolic blood pressure, HOMA-IR, and larger body fat percentage, which were all related to insulin sensitivity. For that reason, rs3856806 was considered as the vital regulation loci of insulin sensitivity.In our study based on Chinese Han population in Beijing district, the sample size was limited; we found that the alleles and genotypes of rs2920503, rs73813168, rs79310821, rs73021485, and rs1702907 in PPARγ had no significant difference between prediabetes/diabetes and normal glucose tolerance, but the genotype-phenotype analysis suggested that subjects with TT genotype of rs2920503 had better insulin secretion function and blood glucose status and subjects with AA/GG genotypes of rs79310821 or with TT/GG genotypes of rs73021485 had better blood glucose status. Studies with a larger sample size are needed to confirm the association of SNPs in PPARγ with diabetes.In summary, this study investigated the association of polymorphism of UCP2 and PPARγ with glucolipid metabolism based on Chinese Han population in Beijing district; it probably could give certain suggestions to prevent insulin resistance in the early stage by classifying the genotype of rs649446 and rs7109266 in UCP2. The polymorphism of PPARγ closely associated with glucolipid metabolism. Subjects with GG genotype of rs2920502 in PPARγ, who had better early- and total-stage insulin secretion function and better serum lipid condition, had a decreased risk for diabetes. Subjects with TT genotype of rs3856806 in PPARγ had an increased risk for diabetes.



---

*Source: 4636783-2018-04-05.xml*