# Functional Evaluation of Upper Urinary Tract with Diuretic Mercaptoacetyltriglycine Renal Scans in Patients with Benign Prostatic Obstruction before and after Surgical Intervention: A Pilot Study

Authors: Sung Yong Cho; Kyungtae Ko; Kyo Chul Koo; Hyung Joon Kim; Woo Jin Bang; Min Soo Choo; Sang Hyub Lee; Young Eun Yoon; Wonho Jung; Jae Young Choi; Dong Sup Lee
Journal: BioMed Research International
 (2020)

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## Abstract

Introduction. We investigated which benign prostatic hyperplasia-related lower urinary parameters are related to upper urinary tract obstruction and whether transurethral prostatectomy could improve upper urinary tract obstruction. Materials and Methods. Patients with prostate size over 30 g and urodynamically proven bladder outlet obstruction were enrolled in this prospective observational study. Bladder wall thickness and prostate size were measured by ultrasonography. A urodynamic study with laboratory tests including serum creatinine, prostate-specific antigen, and urinalysis was performed. Finally, a diuretic scintigraphy using mercaptoacetyltriglycine was performed. Tests except the urodynamic evaluation were repeated after transurethral prostatectomy. Results. In total, 24 patients were enrolled, and 19 patients completed the present study. The mean values of age (yrs), prostate size (mL), bladder thickness (mm), bladder compliance (ΔmL/Δpr), and the bladder outlet obstruction index were 68.42±8.25, 72.29±32.78, 4.42±1.14, 50.17±32.15, and 82.11±34.68, respectively. The mean T1/2 (min) was 17.51±16.34 on the left side and 15.30±11.96 on the right side. Statistical analysis showed that bladder compliance and bladder thickness were preoperatively related to upper urinary tract obstruction (p=0.001 and p=0.007, respectively). Diuretic mercaptoacetyltriglycine scan in 19 patients showed improvement 6 months after prostate surgery. Clinically significant proteinuria was associated with upper urinary tract obstruction, and proteinuria was also improved after prostate surgery. Conclusion. Storage-phase bladder dysfunction could be a reliable urodynamic factor for the indication of upper urinary tract obstruction in patients with benign prostatic hyperplasia, and upper urinary tract obstruction with subsequent kidney damage could be improved by surgical decompression of benign prostatic obstruction.


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## Body

## 1. Introduction

Benign prostatic hyperplasia (BPH) is a prevalent disease in which approximately 50% of men aged 60 have lower urinary tract symptoms [1]. More importantly, BPH is a progressive disease [2] that may result in chronic renal insufficiency as one of the final stages of BPH progression [3]. Nevertheless, it is still unclear whether BPH is a risk factor for chronic kidney disease (CKD) regarding clinical evidence [4, 5]. If BPH causes chronic obstructive uropathy and eventually leads to renal insufficiency, there may be causative anatomical factors or urodynamic factors that could delay urinary drainage from the kidney to the urinary bladder.With respect to the functional evaluation of upper urinary tract obstruction, the diuretic mercaptoacetyltriglycine (99mTc-MAG3) renal scan (hereafter referred to as MAG3) is a feasible imaging tool to evaluate upper urinary tract obstruction [6]. To our knowledge, no clinical study has investigated the relationship of BPH-related lower urinary tract factors and upper urinary tract parameters with MAG3 in patients with BPH. Therefore, the authors conducted a pilot study to investigate whether benign prostatic obstruction (BPO)-related lower urinary tract parameters are related to upper urinary tract obstruction and whether the decompression of BPO could ameliorate upper urinary tract obstruction.


## 2. Materials and Methods

### 2.1. Study Design and the Enrollment of Patients

Patients who complained of lower urinary tract symptoms despite alpha blocker medication were prospectively enrolled from July 2016 to June 2019. Before enrollment, ultrasound (for kidney, bladder, and prostate; bladder thickness was measured at the bladder dome when patients felt the desire to voiding desire), a urodynamic study, and laboratory tests including PSA, serum creatinine with estimated glomerular filtration rate (eGFR) [7], and urinalysis were performed.Patients with a PSA value of 4.0 (ng/mL) or higher underwent ultrasound-guided transrectal prostate biopsy to exclude prostate cancer. When eGFR was <60 ml/min/1.73 m2 or when clinically significant proteinuria was observed, the tests were repeated 3 months later to confirm CKD according to the universal protocol [8], and then, the better result was employed. Laboratory tests were repeated at 6 months after transurethral prostate surgery.International Prostate Symptom Score (IPSS) questionnaire scores evaluated the following seven items were assessed: bladder emptying, frequency, intermittency, urgency, weak stream, straining, and nocturia [9]. The score from each item ranged from 0 to 5. The IPSS questionnaire was repeated 6 months after transurethral prostate surgery.For noninvasive uroflowmetry, patients voided freely without catheterization. At that time, we measured and recorded the maximum flow rate (Qmax) and postvoid residual urine volume (PVR). The PVR was measured using a Biocon 500 ultrasound scanner (Medline Industries, Inc., Mundelein, Illinois, USA). Filling and voiding cystometry was performed, including parameters such as bladder compliance (ΔmL/Δpr), the presence of involuntary detrusor contraction (IDC), the bladder outlet obstruction index (BOOI), Schäfer grades, and the bladder contractility index (BCI) [10]. A urodynamic study was performed only before surgery. Instead, patients were followed up with uroflowmetry 6 months postoperatively.Patients who met all the following inclusion criteria (suggesting BPO) were enrolled in the present study: (1) those with evidence of BOO (BOOI ≥40 or Schäfer grade ≥2); and (2) those with a prostate total volume ≥30 mL in ultrasonography [11–13]. Patients who had any one of the following were excluded from the present study: (1) acute urinary retention during the screening period, (2) urinary tract infection during the screening period, (3) ongoing malignancy, (4) uncontrolled diabetes mellitus (HbA1c >8.0), (5) uncontrolled hypertension (systolic blood pressure >160 mmHg), (6) moderate or severe liver cirrhosis, (7) moderate or severe heart failure (NYHA class ≥ II), or (8) spinal cord disease such as myelopathy.All patients enrolled in the study underwent diuretic MAG3. According to a previous investigation, 5 minutes was normal value of T1/2 in men aged 50~60 years [14]. The authors defined T1/2=10minutes (time point when half of the 99mTc substance is excreted) as the cut-off to distinguish whether the result of the renal scan was negative (<10 minutes) or positive (≥10 minutes), because (1) BPO patients with T1/2 >20 minutes would be very rare unless the obstruction was very severe and (2) 10 minutes is close to the median T1/2 value preoperatively. If the result was positive, the authors suggested it as “potential obstruction.” The MAG3 was postoperatively reevaluated at 6 months. We had also presented the perioperative change with the analysis of the continuous value of MAG3 to compensate the limitation of using the arbitrary cut-off level. Comparison between the mean preoperative MAG3 value and the mean postoperative MAG3 value and correlation between the change of MAG3 value and the other continuous values were performed.To the best of our knowledge, the effect of transurethral prostate surgery for BPO on upper urinary tract drainage has never been reported. The authors analyzed the effect of the transurethral prostate surgery for BPO on the drainage condition as follows. If a 25% or more decrease (at least 1 kidney) was observed in the T1/2 of the postoperative result compared to the T1/2 of the preoperative result, the authors assumed that the upper urinary tract drainage was “relatively” enhanced; furthermore, if a 50% or more decrease (at least 1 kidney) was identified in the T1/2 of MAG3, the authors assumed the upper urinary tract drainage was “definitely” enhanced.


### 2.2. Transurethral Prostate Surgery

Transurethral prostate surgery was performed by surgeons with experience in over 50 cases of holmium laser enucleation of the prostate. Laser energy for enucleation of an adenoma was generally 2.0 J with 20 Hz. The other techniques were similar to those in previously reported methods [15].


### 2.3. Statistics

The authors hypothesized that the upper urinary tract drainage measured by MAG3 could be enhanced 6 months after transurethral surgery in BPO. As a pilot study, two-tailed sign test (binominal test) was used with a beta error of 0.2, an alpha error of 0.05, and an effect size as 0.3 to determine that the target sample size was 20 patients. Assuming a drop-out rate of approximately 20%, we enrolled 24 patients in the present study.The Mann–WhitneyU test and Wilcoxon test were used to check the difference before and 6 months after operation. The chi-squared test or Fisher’s exact test was used to analyze binominal relationships between the groups. Spearman’s test was used to investigate the association among the parameters. All statistical analyses were performed with the statistical package IBM-SPSS for Windows (Version 23.0; IBM Crop, Armonk, NY, USA). p<0.05 was regarded as statistically significant.


## 2.1. Study Design and the Enrollment of Patients

Patients who complained of lower urinary tract symptoms despite alpha blocker medication were prospectively enrolled from July 2016 to June 2019. Before enrollment, ultrasound (for kidney, bladder, and prostate; bladder thickness was measured at the bladder dome when patients felt the desire to voiding desire), a urodynamic study, and laboratory tests including PSA, serum creatinine with estimated glomerular filtration rate (eGFR) [7], and urinalysis were performed.Patients with a PSA value of 4.0 (ng/mL) or higher underwent ultrasound-guided transrectal prostate biopsy to exclude prostate cancer. When eGFR was <60 ml/min/1.73 m2 or when clinically significant proteinuria was observed, the tests were repeated 3 months later to confirm CKD according to the universal protocol [8], and then, the better result was employed. Laboratory tests were repeated at 6 months after transurethral prostate surgery.International Prostate Symptom Score (IPSS) questionnaire scores evaluated the following seven items were assessed: bladder emptying, frequency, intermittency, urgency, weak stream, straining, and nocturia [9]. The score from each item ranged from 0 to 5. The IPSS questionnaire was repeated 6 months after transurethral prostate surgery.For noninvasive uroflowmetry, patients voided freely without catheterization. At that time, we measured and recorded the maximum flow rate (Qmax) and postvoid residual urine volume (PVR). The PVR was measured using a Biocon 500 ultrasound scanner (Medline Industries, Inc., Mundelein, Illinois, USA). Filling and voiding cystometry was performed, including parameters such as bladder compliance (ΔmL/Δpr), the presence of involuntary detrusor contraction (IDC), the bladder outlet obstruction index (BOOI), Schäfer grades, and the bladder contractility index (BCI) [10]. A urodynamic study was performed only before surgery. Instead, patients were followed up with uroflowmetry 6 months postoperatively.Patients who met all the following inclusion criteria (suggesting BPO) were enrolled in the present study: (1) those with evidence of BOO (BOOI ≥40 or Schäfer grade ≥2); and (2) those with a prostate total volume ≥30 mL in ultrasonography [11–13]. Patients who had any one of the following were excluded from the present study: (1) acute urinary retention during the screening period, (2) urinary tract infection during the screening period, (3) ongoing malignancy, (4) uncontrolled diabetes mellitus (HbA1c >8.0), (5) uncontrolled hypertension (systolic blood pressure >160 mmHg), (6) moderate or severe liver cirrhosis, (7) moderate or severe heart failure (NYHA class ≥ II), or (8) spinal cord disease such as myelopathy.All patients enrolled in the study underwent diuretic MAG3. According to a previous investigation, 5 minutes was normal value of T1/2 in men aged 50~60 years [14]. The authors defined T1/2=10minutes (time point when half of the 99mTc substance is excreted) as the cut-off to distinguish whether the result of the renal scan was negative (<10 minutes) or positive (≥10 minutes), because (1) BPO patients with T1/2 >20 minutes would be very rare unless the obstruction was very severe and (2) 10 minutes is close to the median T1/2 value preoperatively. If the result was positive, the authors suggested it as “potential obstruction.” The MAG3 was postoperatively reevaluated at 6 months. We had also presented the perioperative change with the analysis of the continuous value of MAG3 to compensate the limitation of using the arbitrary cut-off level. Comparison between the mean preoperative MAG3 value and the mean postoperative MAG3 value and correlation between the change of MAG3 value and the other continuous values were performed.To the best of our knowledge, the effect of transurethral prostate surgery for BPO on upper urinary tract drainage has never been reported. The authors analyzed the effect of the transurethral prostate surgery for BPO on the drainage condition as follows. If a 25% or more decrease (at least 1 kidney) was observed in the T1/2 of the postoperative result compared to the T1/2 of the preoperative result, the authors assumed that the upper urinary tract drainage was “relatively” enhanced; furthermore, if a 50% or more decrease (at least 1 kidney) was identified in the T1/2 of MAG3, the authors assumed the upper urinary tract drainage was “definitely” enhanced.


## 2.2. Transurethral Prostate Surgery

Transurethral prostate surgery was performed by surgeons with experience in over 50 cases of holmium laser enucleation of the prostate. Laser energy for enucleation of an adenoma was generally 2.0 J with 20 Hz. The other techniques were similar to those in previously reported methods [15].


## 2.3. Statistics

The authors hypothesized that the upper urinary tract drainage measured by MAG3 could be enhanced 6 months after transurethral surgery in BPO. As a pilot study, two-tailed sign test (binominal test) was used with a beta error of 0.2, an alpha error of 0.05, and an effect size as 0.3 to determine that the target sample size was 20 patients. Assuming a drop-out rate of approximately 20%, we enrolled 24 patients in the present study.The Mann–WhitneyU test and Wilcoxon test were used to check the difference before and 6 months after operation. The chi-squared test or Fisher’s exact test was used to analyze binominal relationships between the groups. Spearman’s test was used to investigate the association among the parameters. All statistical analyses were performed with the statistical package IBM-SPSS for Windows (Version 23.0; IBM Crop, Armonk, NY, USA). p<0.05 was regarded as statistically significant.


## 3. Results

In total, 24 patients were enrolled in the present study. Five patients were excluded due to the postoperative confirmation of pathologically-proven prostate cancer in 3 patients, loss to follow-up in one patient, and cervical myelopathy discovered during follow-up in 1 patient. In total, 19 patients completed the study.The mean age was68.42±8.25 years, and the mean BMI was 23.83±1.66kg/m2. The baseline patient characteristics are shown in Table 1.Table 1
Patients’ baseline characteristics (n=19).
Age (years)68.42±8.25Body mass index (kg/m2)23.83±1.66Prostate-specific antigen (ng/mL)6.67±4.52Serum creatinine (mg/dL)1.25±0.90eGFR (mL/min/1.73m2)71.68±19.43ProteinuriaTrace or more15/19 (78.9%)+1 or more3/19 (15.8%)Uroflowmetry parametersQmax (mL/sec)8.51±4.91Voiding volume (mL)181.11±96.74Postvoid residual volume (mL)98.53±89.92Invasive urodynamic parametersBladder compliance (ΔmL/Δpr)50.17±32.15Involuntary detrusor contraction2/19 (10.5%)Bladder outlet obstruction index82.11±34.68Schäfer grades3.95±1.47Bladder contractility index114.45±40.24Anatomical factorsProstate size (mL)72.29±32.78Bladder thickness (mm)4.42±1.14IPSS total score22.44±7.51Storage symptom score8.72±3.88Obstructive symptom score13.72±4.03Quality of life score4.78±0.73T1/2 of 99mTc-MAG3Left side17.51±16.34Right side15.30±11.96T1/2 >10 min at least one kidney13/19 (68.4%)Underlying conditionsDiabetes mellitus4/19 (21.1%)Hypertension7/19 (36.8%)Smoking5/19 (26.3%)Under 20 p∗yrs2/19 (10.5%)Over 20 p∗yrs3/19 (15.8%)Education (high school or more)13/19 (68.4%)Recent symptom aggravation (year)1.33±1.09Previous history of acute urinary retention3/19 (15.8%)Parameters were expressed by themean±standarddeviation or frequency (%). eGFR: estimated glomerular filtration rate; Qmax: maximal flow rate; IPSS: international prostate symptom score; MAG-3: diuretic mercaptoacetyltriglycine.Among 19 patients with BPO, a preoperative T1/2 of MAG3 over 10 minutes (potential obstruction) was observed in 13 patients (21 kidneys). Preoperative potential obstruction in at least 1 kidney was associated with clinically significant proteinuria (Spearman’srho=0.482, p=0.036). The associations between preoperative potential obstruction in at least 1 kidney and other factors are shown in Table 2.Table 2
Association between potential obstruction1 and other factors in preoperative data.
Upper tract factorClinically significant proteinuria0.482,p=0.036∗eGFR0.083,p=0.736Lower tract factorBladder thickness0.614,p=0.007∗Prostate size-0.269,p=0.266Urodynamic factorStorage factorsVoiding volume-0.310,p=0.196Bladder compliance-0.704,p=0.001∗Obstructive factorsQmax-0.021,p=0.933Postvoid residual volume-0.145,p=0.554Bladder outlet obstruction index0.041,p=0.866Other factorBladder contractility index-0.031,p=0.583General factorsAge-0.031,p=0.899Body mass index-0.165,p=0.499Diabetes mellitus-0.205,p=0.401Hypertension-0.049,p=0.841Data were expressed by Spearman’s rho withp value. ∗p<0.05. 1: potential obstruction means preoperative T1/2 of 99mTc-MAG3 >10 minutes at least 1 kidney.At 6 months after transurethral prostate surgery, potential obstruction remained in 6 patients (9 kidneys). Based on the aforementioned criteria, “relative” improvement of upper urinary tract drainage was observed in 16 patients (26 kidneys), and “definite” improvement was seen in 4 patients (6 kidneys).All laboratory tests, including prostate-specific antigen, eGFR, and urinary proteinuria, were improved after transurethral prostate surgery. The mean prostate-specific antigen level decreased from6.67±4.52 to 1.55±1.44. The mean QoL scores in the IPSS questionnaire improved from 4.78±0.73 to 1.11±0.76. The other results from the comparison between preoperative values and postoperative values are shown in Figure 1. Although voiding volume did not increase significantly, Qmax was remarkably improved, and postvoid residual volume decreased obviously.Figure 1
Changes in parameters before and at 6 months after the decompression of BPO. (a, b) Renal function was improved. (c, d) The T1/2 of diuretic 99mTc-MAG3 was improved. (e–g) The mean values of Qmax, voiding volume, and PVR seemed to be enhanced, but voiding volume did not significantly increase. (h) The IPSS score was markedly improved. BPO: benign prostatic obstruction; PVR: postvoid residual urine volume; IPSS: international prostate symptom score. Asterisk (∗) means p<0.05.
(a)(b)(c)(d)(e)(f)(g)(h)As lower urinary factors such as bladder compliance and bladder thickness were related to the T1/2 of MAG3, the authors investigated the likelihood of improvement in the T1/2 of MAG3 based on bladder compliance and bladder thickness. Because most patients with preoperatively good compliance (less thickened bladder) had a T1/2 of MAG3 under 10 minutes, the value did not change very much at 6 months after operation, whereas BPO patients who had preoperatively low compliance with thickened bladder and a tendency for delay in T1/2 had much room for improvement in the T1/2 of MAG3 (Figures 2 and 3). This observation means that transurethral prostate surgery in BPO patients with low compliance and/or thickened bladder may be beneficial to improve upper urinary tract drainage.Figure 2
Changes in the T1/2 of the diuretic 99mTc-MAG3 scan according to preoperative bladder compliance and bladder thickness. (a, b) The preoperative bladder compliance was lower, and the change in the T1/2 of the diuretic 99mTc-MAG3 scan was greater. (c, d) The preoperative bladder was thicker, and the change in the diuretic 99mTc-MAG3 scan was greater.
(a)(b)(c)(d)Figure 3
Changes in the T1/2 of the diuretic 99mTc-MAG3 scan according to the preoperative T1/2 of the diuretic 99mTc-MAG3 scan results. (a, b) Preoperative values of the T1/2 of the diuretic 99mTc-MAG3 scan were 7.0 in the left kidney and 9.3 in the right kidney, which means no delay in the T1/2 of the upper urinary tract (a). The values were improved to 4.7 in the left kidney and 5.3 in the right kidney postoperatively (b). (c, d) Preoperative values of the T1/2 of the diuretic 99mTc-MAG3 scan were 73.3 in the left kidney and 44.0 in the right kidney, which means obvious upper urinary tract obstruction (c). The values were markedly improved to 11.6 in the left kidney and 11.6 in the right kidney postoperatively (d).
(a)(b)(c)(d)Clinically, significant proteinuria remained in 4 patients (preoperative proteinuria was found in 15 patients), and potential obstruction was still observed in 6 patients (preoperative potential obstruction was observed in 13 patients) at 6 months after the operation. In the correlation test, postoperative proteinuria was related not to upper urinary tract obstruction but to underlying diabetes mellitus (Table3).Table 3
Association between postoperative significant proteinuria and other factors in postoperative data.
Upper tract factorPotential obstruction10.233,p=0.338eGFR-0.313,p=0.191Lower tract factorUroflowmetryQmax-0.093,p=0.706Voiding volume-0.054,p=0.825Postvoid residual volume-0.440,p=0.059General factorsAge0.118,p=0.630Body mass index-0.163,p=0.505Diabetes mellitus0.696,p=0.001∗Hypertension0.420,p=0.073Data were expressed by Spearman’s rho withp value. ∗p<0.05. 1: potential obstruction means postoperative T1/2 of 99mTc-MAG3 >10 minutes at least 1 kidney.


## 4. Discussion

The interesting finding of this study is that filling-phase bladder dysfunction, such as low compliance, could explain the occurrence of obstruction in the MAG3 in the present cohort. A similar study was conducted with pediatric neurogenic bladders, where the authors emphasized low bladder compliance and bladder wall thickness as a predictor of renal damage [16]. Many clinical studies support the likelihood of an association between bladder wall thickness and BPO [17], describing that the measurement of bladder wall thickness has a median sensitivity of 82% and a specificity of 92% for the prediction of BOO. De Nunzio et al. found that detrusor overactivity was significantly associated with bladder wall thickness even in non-BPO patients [18]. Therefore, the thickened bladder is not an exclusive property of BOO (or BPO); rather, it may be more related to detrusor condition regardless of BPO. Consequently, chronic BOO secondary to BPH can be a nonneurogenic factor among various lower urinary tract conditions that thicken the urinary bladder wall [19].The BOOI was not significantly associated with the status of the upper urinary tract drainage measured by the T1/2 of MAG3. Instead, only bladder compliance was responsible for upper urinary tract drainage in the present study. Two cases of IDC also showed a delay in the T1/2 of MAG3. These findings indicated that BOO itself would not induce obstruction in the upper urinary tract, but the long-standing high intravesical pressure induced by storage-phase bladder dysfunction might be the reason for upper urinary tract obstruction. Although the number of patients included is small, the authors suggest that BPH might not be the direct cause of renal insufficiency and BPH may be one of the factors that may induce bladder dysfunction, such as low compliance, which can affect urine drainage. This may also mean that the transient elevation of intravesical pressure during voiding in patients with BPH or a high BOOI without abnormalities in the filling phase would not induce renal insufficiency. This finding is consistent with previous investigations with neurogenic bladder [20, 21].Considering that PdetQmax is incorporated in the BCI and the BOOI, it is possible that detrusor contraction increased to overcome the large size of the prostate. Nevertheless, the BCI was also not related to the T1/2 of MAG3. Because evidence of BOO (with a BOOI of 40 or more) was a criterion for inclusion in the present study, the bladder contractility in the majority of patients was intact or relatively preserved. According to several suggested criteria for detrusor underactivity (DU) [22, 23], PdetQmax should be under 30 cmH2O. Therefore, no one could have had DU in this cohort. However, researchers should be aware that either BPO without DU or impaired detrusor activity can cause chronic urinary retention (CUR) [24], and CUR can be associated with hydronephrosis [25]. CUR is a clinical definition (not by urodynamic study), and most of the literature indicates CUR as PVR over 300 mL [25]. In the present study, only 1 case of CUR was shown in a patient with a prostate size of 58.5 mL, BOOI of 48, BCI of 55, bladder compliance of 33, and T1/2 of MAG3 over 10 minutes in the left kidney. It indicates that the patient had high-pressure CUR (BPO without DU) [26]. Therefore, one of the limitations of the present study is that the authors cannot determine whether DU in BPH patients (BPH with low-pressure CUR) could also indicate a connection between bladder compliance and a delay in the T1/2 of MAG3 in terms of CUR.Prostate size was not associated with the T1/2 of MAG3, as the BOOI and BCI were not related to the T1/2 of MAG3. Previous studies have already suggested that prostate size might not be related to the decrease in renal function [5, 27]. Consequently, the authors believe that prostate size and relevant functional parameters, such as the BOOI and/or BCI, could not directly influence the delay in urinary drainage from the upper urinary tract because the urinary bladder acts as a “buffer.” In the case of a loss of flexibility of the buffer (low compliance) or of a fullness of the buffer (CUR), the end-organ (BPO) effect could develop. Therefore, preoperative urodynamic evaluation in BPH is very important.It was very interesting that preoperative proteinuria was associated with a delay in the T1/2 of MAG3. A recent study showed that clinically significant proteinuria was more frequently identified in BPO patients with low bladder compliance [28], and the authors found that transurethral prostate surgery for those patients mitigated proteinuria and that the phenomenon was obvious in patients without underlying medical disease. Considering that proteinuria is a marker of ongoing renal damage [29], the present study revealed the probability that BPO with functionally low bladder compliance and an anatomically thickened bladder wall may delay the T1/2 of MAG3, resulting in renal damage (proteinuria) even in patients without CUR.The postoperative T1/2 of MAG3 was not related to the remaining proteinuria at 6 months postoperatively, which indicated that underlying hypertension and/or DM might be more responsible for the remaining proteinuria (Table 3). Conversely, BPO could be involved in the pathophysiology of developing CKD. Considering CKD can also be multifactorial, we could not definitely rule out intrinsic renal causes presenting proteinuria because we did not perform or recommend renal biopsy in the present cohort, which could be a limitation of the study. Nevertheless, in the present study, proteinuria, eGFR, and the T1/2 of MAG3 were significantly improved after surgery. The authors think that the preoperative discordance between eGFR and T1/2 of MAG3 might be due to the following reasons: (1) the baseline eGFRs were close-set one another; (2) individual baseline eGFR could be determined not only by the urinary drainage condition but also by various factors such as age and underlying diseases including hypertension and DM; and (3) the decrease in eGFR is a quite late response compared to the development of significant proteinuria [30].Nomura et al. showed urodynamic results in patients who underwent transurethral prostate surgery. Half of those patients with detrusor overactivity improved 6 months after operation [31]. Furthermore, an interesting result was reported by Tkocz et al. that transurethral resection or incision of the prostate could sufficiently improve bladder compliance as well as remarkably decrease the incidence of detrusor overactivity unless the detrusor structure had irreversibly changed [32]. The absence of postoperative urodynamic analysis was a major limitation of the present study, but the preoperative relationship between the T1/2 of MAG3 and bladder compliance and the improvement in the T1/2 of MAG3 could support our assumption for the improvement in postoperative bladder compliance.Meanwhile, 5 alpha reductase inhibitors (5ARIs) can be used for pharmacological deobstruction for BPO. According to the early research conducted by Stoner who used finasteride for BPH treatment for 36 months, he found that the prostate volume was reduced by 27% compared to baseline (over 45 mL), and Qmax was improved by 2.3 mL/s [33]. Similarly, in CombaT study, even with 24 months of combination therapy (dutasteride plus tamsulosin), Qmax improved by 2.2~3.0 mL/s [34]. Although pharmacological deobstruction using 5ARIs (with or without alpha 1 selective adrenoreceptor antagonist) could be beneficial to relieve the BPO, (1) it takes too much time to observe the results, and (2) the change of parameters such as increase in Qmax and decrease in prostate volume may be tiny, compared to surgical decompression. Therefore, the pharmacological deobstruction seems to be inappropriate as a control group of a study evaluating the MAG3 parameter.Finally, the present study contains severe forms of BPH, with a mean bladder compliance, BOOI, prostate size, and IPSS score of 50.17, 82.11, 72.29, and 22.44, respectively, which cautions that the data of this cohort cannot be generalized for all BPH patients. However, the present study showed the importance of bladder function, which was central to the explanation of the probability of CKD progression in BPO patients, and provided a message of the importance of decompression of BPO in the prevention of kidney damage due to the delay in urinary drainage.


## 5. Conclusions

In conclusion, storage-phase bladder dysfunction (low compliance and/or detrusor overactivity) may be a key factor for kidney damage resulting from delayed urinary drainage in patients with BPO. Such a delay could be improved by the decompression of BPO. Based on the results of the present study with supporting literature, the authors suggest that the role of BPH progression in the development of CKD is as follows: “BPH ➔ BOO ➔ storage-phase bladder dysfunction with bladder wall thickness ➔ delay in upper urinary tract drainage ➔ kidney damage (proteinuria) ➔ CKD progression”. Therefore, when surgeons encounter patients with severe BPH, they should seriously consider preoperative urodynamic studies to evaluate storage-phase bladder function. Given the cost of diuretic renal scans, surgeons should also perform urinalysis in those patients to detect proteinuria before and after surgery.



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