# Effect of Shin’iseihaito (Xinyiqingfeitang) on AcuteStreptococcus pneumoniae Murine Sinusitis via Macrophage Activation

Authors: Masaaki Minami; Toru Konishi; Hiroshi Takase; Zhixia Jiang; Tetsuya Arai; Toshiaki Makino
Journal: Evidence-Based Complementary and Alternative Medicine
 (2017)

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## Abstract

Streptococcus pneumoniae (S. pneumoniae) causes sinusitis. The general treatment ofS. pneumoniasinusitis is by using antibiotics; however, one of their serious problems is the attenuation of their effect. Shin’iseihaito (Xinyiqingfeitang), a formula of Japanese traditional Kampo medicine, has been used for the treatment of sinusitis in Japan. In this study, we investigated the efficacy of Shin’iseihaito againstS. pneumoniae-caused sinusitis in mice. Oral administration of Shin’iseihaito extract (SSHT) decreased the nasal colonization ofS. pneumoniaein both prophylactic and therapeutic treatments, respectively, and the former was more effective than the latter. Histopathological analysis revealed that the epithelial tissue fromS. pneumoniae-infected nose under SSHT treatment recovered the tissue destruction in comparison to infected nose. We also confirmed this result by scanning electron microscopic analysis. Murine peritoneal macrophages from SSHT-treated mice had significant phagocytic activity in comparison to those from untreated group. We also found that tumor necrosis factor-α, interleukin-1β, interleukin-6, and monocyte chemotactic protein-1 levels and the migration of macrophages fromS. pneumoniae-infected mice with the treatment with SSHT were increased compared to those from untreated group. Our data suggest that Shin’iseihaito may be useful for the treatment ofS. pneumoniae-induced sinusitis.


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## Body

## 1. Introduction

Streptococcus pneumoniae (S. pneumoniae) is the gram-positive bacterium which is the most common pathogen causing sinusitis, otitis media, pneumonia, meningitis, and sepsis, especially in young children and the elderly [1]. Although antibiotics such as penicillin have been effective againstS. pneumoniae for long time, several serious problems againstS. pneumoniae therapy have occurred. One of them is the attenuation of the antibiotic effect. A number of antimicrobials that were previously useful in the treatment of acute bacterial sinusitis had MIC90s (Minimum Inhibitory Concentrations 90%) against intermediately resistantS. pneumoniae that were not achievable [2]. Furthermore, recently, multidrug-resistantS. pneumoniae has been increasing in Japan and worldwide [3]. AsS. pneumoniae get antibacterial activity gradually, new anti-infective therapy againstS. pneumoniae is desired.Acute sinusitis is a common disease characterized by recurrent or persistent inflammation of the nasal and the paranasal sinus mucosa. It can be defined as an inflammation of paranasal sinus mucosa less than 3 weeks in duration [4]. As most acute cases result from infection, there is evidence that, in acute bacterial and viral sinusitis, proinflammatory cytokines play a dominant role in initiating and maintaining the inflammation, which is characterized by neutrophil tissue infiltration [5].Traditional Chinese medicine (TCM) is one of the most popular alternative, complementary therapies worldwide [6]. It is becoming a popular alternative in otorhinolaryngology, where its use in the treatment of sinusitis, tinnitus, deafness, and Meniere’s disease is growing [7]. In Japan, Kampo medicine, which had been developed from ancient Chinese medicine, is recognized as an effective alternative medicine against several diseases [8, 9]. Shin’iseihaito (Xinyiqingfeitang) is a formula in both traditional Japanese Kampo medicine and Chinese medicine, which is used for the treatment of upper respiratory tract diseases, especially sinusitis, in Japan [10, 11]. In Taiwan, the clinical investigation of the characteristics of adjunctive traditional Chinese medicine use in patients with chronic rhinosinusitis demonstrated that the most common Chinese herbal formula used was Xinyiqingfeitang [12]. In our previous basic studies, we investigated the antimicrobial effect of Shin’iseihaito extract (SSHT) in a pneumococcus-infected model [13], the antibacterial activity of SSHT againstS. pneumoniae in vitro [14], and the preventive effect of SSHT in an ovalbumin-induced allergic rhinitis model [15]. However, experimental evidences on the use of SSHT for the treatment of bacterial sinusitis such asS. pneumoniae infection have not been elucidated yet.In the present study, we evaluated the efficacy of SSHT againstS. pneumoniae using a nasal infection murine model like sinusitis. Furthermore, we also evaluated the phagocytosis and chemotaxis effect of murine macrophage from SSHT-treated mouse.


## 2. Materials and Methods

### 2.1. Bacterial Strains

Streptococcus pneumoniae (S. pneumoniae) (ATCC49619) (American Type Culture Collection, Rockville, MD, USA) was used in this study. ATCC49619 (cps type: 19A) was isolated from sputum of a 75-year-old male. A fresh colony was inoculated overnight on TSAII sheep blood agar (Nihon Becton Dickinson, Tokyo, Japan) and cultured for 16 hours at 37°C under 5% CO2. The bacteria were harvested by centrifugation and resuspended in sterile phosphate-buffered saline (0.15 M, pH 7.2, PBS). Bacterial density was determined by measuring the absorbance at 600 nm (A600). The bacterial suspension was then diluted with PBS to 109 CFU (colony forming unit)/mL using a standard growth curve to relate measured A600 to bacterial concentration.


### 2.2. Crude Drugs

Shin’iseihaito (Xinyiqingfeitang) (daily dose for human) consists of 3.0 g of the rhizome ofAnemarrhena asphodeloides (AA), 1.0 g of the rhizome ofCimicifuga heracleifolia (CH), 2.0 g of the leaf ofEriobotrya japonica(EJ), 5.0 g ofGypsum fibrosum(GF), 3.0 g of the fruit ofGardenia jasminoides(GJ), 3.0 g of the bulb ofLilium lancifolium(LL), 3.0 g of the flower ofMagnolia salicifolia(MS), 5.0 g of the tuber ofOphiopogon japonicas(OJ), and 3.0 g of the root ofScutellaria baicalensis(SB). These crude drugs were purchased from Daikoshoyaku (Nagoya, Japan) or Tsumura (Tokyo, Japan) and were standardized by Japanese Pharmacopoeia 17th Edition [16]. The mixture of above crude drugs was boiled in 20 times the weight of water for 30 min and filtered. The decoction was lyophilized to yield powdered extract (SSHT, 7.1 g for daily human dose). Fingerprint pattern of this SSHT was shown in our previous study [14]. SSHT was suspended in distilled water to prepare the stock solution at a concentration of 0.1 g/mL and kept in −20°C until use.


### 2.3. Murine Model of Nasal Infection

The ability of prophylactic effect ofS. pneumoniae to cause sinusitis in mice after nasal inoculation was assessed using a procedure as follows [17]. In brief,S. pneumoniae was harvested after 16-hour growth on TSAII sheep blood agar and was mixed in 1 mL of PBS and then centrifuged at 2,000 ×g for 2 min. The pellets were diluted in 100 μL PBS to 1 × 108 CFU and then inoculated into the both nostrils of inbred 5-week-old male ddY mice (Japan SLC, Shizuoka, Japan) using a 29-gauge needle. The number of CFU inoculated was verified for each experiment by plating the bacteria on TSAII sheep blood agar and counting CFU. Mice were observed daily. In the SSHT-treated group, mice were gavaged with SSHT (0.69, 1.39, or 2.9 g/kg body weight (bw)) on days −1, 0, 1, 2, and 3 afterS. pneumoniae inoculation (Figure 1(a)). We also performed another protocol to clarify the therapeutic effects of SSHT. In SSHT-treated group, mice were gavaged with SSHT (2.9 g/kg bw) on days 1, 2, and 3 afterS. pneumoniaeinoculation (Figure 1(b)). Mice in SSHT-untreated group for both protocols were given an equal volume of PBS instead of SSHT and were infected using the same method. The mice in control group for prophylactic protocol were given PBS without infection.Figure 1
Protocols for the prophylactic (a) and therapeutic (b) experiments ofS. pneumoniae-induced murine sinusitis model. In infected group, 1 × 107 CFU bacteria were injected into both nostrils of mice using a 29-gauge needle at day 0. In the SSHT-treated group, mice were gavaged with Shin’iseihaito extract (SSHT) (2.9 g/kg body weight (bw))/day.
(a)
(b)


### 2.4. Nasal Lavage Cultures

The procedure of nasal cultures was described elsewhere [17]. In brief, the mice were sacrificed by CO2 inhalation. After that, the external nares, oral cavity, and head were disinfected with a moist alcohol swab and allowed to dry. Nasal lavage was performed with 200 μL of PBS. The recovered fluid was then serially diluted, and 10 μL of each dilution was plated onto TSAII sheep blood agar plates. The plates were incubated for 24 h, and then colonies ofS. pneumoniaewere counted. The results were quantified as the number of CFU/mL.


### 2.5. Histological Preparation

The histological preparation was assessed using a procedure described elsewhere [18]. Briefly, the head of mouse was stripped of eyes, skin, and muscle under low magnification, and the mandibles and tongue were discarded. After the head was then soaked in fixative overnight, it was decalcified and was trimmed with a fresh razor blade. The resulting blocks were embedded in paraffin sectioned anterior to posterior at 5 μm thickness and stained with hematoxylin-eosin (H&E) method.


### 2.6. Scanning Electron Microscopic Analysis

Scanning electron microscopic preparation was performed according to what was described elsewhere [19]. Briefly, the head of mouse was stripped of eyes, skin, and muscle under low magnification, and the mandibles and tongue were discarded. Tissue was taken only if the debridement of the tissue was necessary during the surgical treatment. The tissue samples were immediately placed in 2.5% glutaraldehyde (Nisshin EM, Tokyo, Japan) (prepared in 0.1 M phosphate buffer, pH 7.4) for 24 h at 4°C as a prefixation step. They were then rinsed twice with 0.1 M phosphate buffer (pH 7.4), postfixed using 2% osmium tetroxide (Nisshin EM, Tokyo, Japan) for 2 h at room temperature, and finally rinsed with distilled water. Next, the specimens were dehydrated using graduated concentrations of ethyl alcohol (30%, 50%, 70%, 90%, 95%, and 100%) for 30 min, each followed by absolute alcohol for 30 min. The specimen was dried using the critical point dryer CPD300 (Leica, Wetzlar, Germany). For mounting, carbon conductive paint was used; for specimens, osmium coating with Osmium Coater (NL-OPC-AJ, Filgen, Nagoya, Japan) was used. Finally, each specimen was examined using a scanning electron microscope (SEM) (Hitachi High-Technologies S-4800). Several areas of each sample were systematically scanned.


### 2.7. Ex Vivo Isolation of SSHT-Treated Macrophage Cells

The procedure of ex vivo isolation of macrophage cells collected from SSHT-treated mice was modified as previous report [20]. In the SSHT-treated group, mice were gavaged with SSHT (2.9 g/kg body weight (bw)) for 4 days. Mice in the untreated group were given an equal volume of PBS. Then, peritoneal macrophage cells were harvested by lavage with 6 mL of cold sterilized PBS from the peritoneal cavities. The cells were washed twice and suspended in culture medium (RPMI1640 (Wako Pure Chemical Industry, Osaka, Japan) supplemented with 5% fetal bovine serum (FBS, Sigma-Aldrich, St. Louis, MO, USA)) to give 106 cells/mL. 100 μL of the cell suspension was dispensed into 1.5 mL tube and incubated for 2 h at 37°C in 5% CO2. The culture medium was centrifuged and removed gently by aspiration and changed to fresh culture medium. All cells were suspended in the culture medium, plated onto 24-well culture plate at dose of 1 × 105 cells per well (final volume, 0.1 mL), and cultured in a humidified chamber at 37°C with 5% CO2 atmosphere for 24 h.


### 2.8. Determination of Macrophage Proliferative Response

At 20 h before the end of the macrophage culture,3H-thymidine (2.0 Ci/mmol; PerkinElmer, MA, USA) was added to the medium in the wells. When the culture was finished, the cells were adsorbed on 0.45 μm membrane filters (Advantech Japan, Tokyo, Japan), washed with distilled water, and then dried. The filters were transferred to vials filled with liquid scintillator cocktail, and the radioactivity was measured with a liquid scintillation counter (LSC-6100, Hitachi Aloka Medical, Tokyo, Japan). Results are given as D.P.M. ± SD of triplicate samples.


### 2.9. Macrophage Phagocytic Assay

The procedure of macrophage phagocytic assay was modified as previous report [20]. Mouse macrophage (2 × 106 cells in 100 μL) andS. pneumoniae(104 CFU in 100 μL) that was opsonized with mouse serum for 30 min were incubated at 37°C for 1 h with shaking on a rotator in 5% CO2 atmosphere. Then, the aliquots were plated on TSAII sheep blood agar to determine the number of CFU after 0, 30, 60, 90, and 120 min. Data were expressed as bacterial CFU counts at each time.


### 2.10. Cytokine Production Analysis by ELISA

Peritoneal macrophages were cultured in a 24-well (1 × 105/well) plate in RPMI1640 containing 5% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin (GE Healthcare, Chicago, IL, USA) and incubated for 24 h. Then, the medium was collected, and the concentrations of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), IL-6, and monocyte chemotactic protein-1 (MCP-1) were measured using enzyme-linked immunosorbent assay (ELISA) kits (BioLegend, San Diego, CA, USA).


### 2.11. Macrophage Chemotaxis Assay

Chemotaxis of peritoneal macrophages was assayed using 24-well chemotactic chambers (pore size, 5μm; Costar, ME, USA). The Zymosan-activated serum was prepared by adding 10 mg of Zymosan (Sigma) to 1 mL of FBS and incubation at 37°C for 30 min. After incubation, the serum was centrifuged at 1,000 ×g for 10 min and diluted with PBS to give a 20% solution [21]. The upper chamber compartment was loaded with 100 μL mouse peritoneal macrophage suspension (1 × 106 cells). The lower compartment was filled with 900 μL RPMI1640 medium with both 2% FBS and Zymosan-activated serum. Following 24 h incubation at 37°C with 5% CO2 atmosphere, the lower surface of chambers was stained with Giemsa staining, and the number of cells that had migrated to the lower surface of chamber was counted by fluorescence microscope (Eclipse E800, Nikon Instruments Co., Ltd., Tokyo). Data was determined as the mean of six random high-power fields per well. Each assay was performed at least three times. The chemotactic index was the ratio of the number of cells that had migrated in response to Zymosan-activated serum to the unmigrated number of cells as described before [22].


### 2.12. Statistical Analysis

The statistical analysis was conducted using an unpaired Student’st-test for two groups and Tukey’s multiple comparison test for the differences among multiple groups or two-way ANOVA followed by Tukey’s test for time course study of 3 groups. p values less than 0.05 were considered statistically significant.


## 2.1. Bacterial Strains

Streptococcus pneumoniae (S. pneumoniae) (ATCC49619) (American Type Culture Collection, Rockville, MD, USA) was used in this study. ATCC49619 (cps type: 19A) was isolated from sputum of a 75-year-old male. A fresh colony was inoculated overnight on TSAII sheep blood agar (Nihon Becton Dickinson, Tokyo, Japan) and cultured for 16 hours at 37°C under 5% CO2. The bacteria were harvested by centrifugation and resuspended in sterile phosphate-buffered saline (0.15 M, pH 7.2, PBS). Bacterial density was determined by measuring the absorbance at 600 nm (A600). The bacterial suspension was then diluted with PBS to 109 CFU (colony forming unit)/mL using a standard growth curve to relate measured A600 to bacterial concentration.


## 2.2. Crude Drugs

Shin’iseihaito (Xinyiqingfeitang) (daily dose for human) consists of 3.0 g of the rhizome ofAnemarrhena asphodeloides (AA), 1.0 g of the rhizome ofCimicifuga heracleifolia (CH), 2.0 g of the leaf ofEriobotrya japonica(EJ), 5.0 g ofGypsum fibrosum(GF), 3.0 g of the fruit ofGardenia jasminoides(GJ), 3.0 g of the bulb ofLilium lancifolium(LL), 3.0 g of the flower ofMagnolia salicifolia(MS), 5.0 g of the tuber ofOphiopogon japonicas(OJ), and 3.0 g of the root ofScutellaria baicalensis(SB). These crude drugs were purchased from Daikoshoyaku (Nagoya, Japan) or Tsumura (Tokyo, Japan) and were standardized by Japanese Pharmacopoeia 17th Edition [16]. The mixture of above crude drugs was boiled in 20 times the weight of water for 30 min and filtered. The decoction was lyophilized to yield powdered extract (SSHT, 7.1 g for daily human dose). Fingerprint pattern of this SSHT was shown in our previous study [14]. SSHT was suspended in distilled water to prepare the stock solution at a concentration of 0.1 g/mL and kept in −20°C until use.


## 2.3. Murine Model of Nasal Infection

The ability of prophylactic effect ofS. pneumoniae to cause sinusitis in mice after nasal inoculation was assessed using a procedure as follows [17]. In brief,S. pneumoniae was harvested after 16-hour growth on TSAII sheep blood agar and was mixed in 1 mL of PBS and then centrifuged at 2,000 ×g for 2 min. The pellets were diluted in 100 μL PBS to 1 × 108 CFU and then inoculated into the both nostrils of inbred 5-week-old male ddY mice (Japan SLC, Shizuoka, Japan) using a 29-gauge needle. The number of CFU inoculated was verified for each experiment by plating the bacteria on TSAII sheep blood agar and counting CFU. Mice were observed daily. In the SSHT-treated group, mice were gavaged with SSHT (0.69, 1.39, or 2.9 g/kg body weight (bw)) on days −1, 0, 1, 2, and 3 afterS. pneumoniae inoculation (Figure 1(a)). We also performed another protocol to clarify the therapeutic effects of SSHT. In SSHT-treated group, mice were gavaged with SSHT (2.9 g/kg bw) on days 1, 2, and 3 afterS. pneumoniaeinoculation (Figure 1(b)). Mice in SSHT-untreated group for both protocols were given an equal volume of PBS instead of SSHT and were infected using the same method. The mice in control group for prophylactic protocol were given PBS without infection.Figure 1
Protocols for the prophylactic (a) and therapeutic (b) experiments ofS. pneumoniae-induced murine sinusitis model. In infected group, 1 × 107 CFU bacteria were injected into both nostrils of mice using a 29-gauge needle at day 0. In the SSHT-treated group, mice were gavaged with Shin’iseihaito extract (SSHT) (2.9 g/kg body weight (bw))/day.
(a)
(b)


## 2.4. Nasal Lavage Cultures

The procedure of nasal cultures was described elsewhere [17]. In brief, the mice were sacrificed by CO2 inhalation. After that, the external nares, oral cavity, and head were disinfected with a moist alcohol swab and allowed to dry. Nasal lavage was performed with 200 μL of PBS. The recovered fluid was then serially diluted, and 10 μL of each dilution was plated onto TSAII sheep blood agar plates. The plates were incubated for 24 h, and then colonies ofS. pneumoniaewere counted. The results were quantified as the number of CFU/mL.


## 2.5. Histological Preparation

The histological preparation was assessed using a procedure described elsewhere [18]. Briefly, the head of mouse was stripped of eyes, skin, and muscle under low magnification, and the mandibles and tongue were discarded. After the head was then soaked in fixative overnight, it was decalcified and was trimmed with a fresh razor blade. The resulting blocks were embedded in paraffin sectioned anterior to posterior at 5 μm thickness and stained with hematoxylin-eosin (H&E) method.


## 2.6. Scanning Electron Microscopic Analysis

Scanning electron microscopic preparation was performed according to what was described elsewhere [19]. Briefly, the head of mouse was stripped of eyes, skin, and muscle under low magnification, and the mandibles and tongue were discarded. Tissue was taken only if the debridement of the tissue was necessary during the surgical treatment. The tissue samples were immediately placed in 2.5% glutaraldehyde (Nisshin EM, Tokyo, Japan) (prepared in 0.1 M phosphate buffer, pH 7.4) for 24 h at 4°C as a prefixation step. They were then rinsed twice with 0.1 M phosphate buffer (pH 7.4), postfixed using 2% osmium tetroxide (Nisshin EM, Tokyo, Japan) for 2 h at room temperature, and finally rinsed with distilled water. Next, the specimens were dehydrated using graduated concentrations of ethyl alcohol (30%, 50%, 70%, 90%, 95%, and 100%) for 30 min, each followed by absolute alcohol for 30 min. The specimen was dried using the critical point dryer CPD300 (Leica, Wetzlar, Germany). For mounting, carbon conductive paint was used; for specimens, osmium coating with Osmium Coater (NL-OPC-AJ, Filgen, Nagoya, Japan) was used. Finally, each specimen was examined using a scanning electron microscope (SEM) (Hitachi High-Technologies S-4800). Several areas of each sample were systematically scanned.


## 2.7. Ex Vivo Isolation of SSHT-Treated Macrophage Cells

The procedure of ex vivo isolation of macrophage cells collected from SSHT-treated mice was modified as previous report [20]. In the SSHT-treated group, mice were gavaged with SSHT (2.9 g/kg body weight (bw)) for 4 days. Mice in the untreated group were given an equal volume of PBS. Then, peritoneal macrophage cells were harvested by lavage with 6 mL of cold sterilized PBS from the peritoneal cavities. The cells were washed twice and suspended in culture medium (RPMI1640 (Wako Pure Chemical Industry, Osaka, Japan) supplemented with 5% fetal bovine serum (FBS, Sigma-Aldrich, St. Louis, MO, USA)) to give 106 cells/mL. 100 μL of the cell suspension was dispensed into 1.5 mL tube and incubated for 2 h at 37°C in 5% CO2. The culture medium was centrifuged and removed gently by aspiration and changed to fresh culture medium. All cells were suspended in the culture medium, plated onto 24-well culture plate at dose of 1 × 105 cells per well (final volume, 0.1 mL), and cultured in a humidified chamber at 37°C with 5% CO2 atmosphere for 24 h.


## 2.8. Determination of Macrophage Proliferative Response

At 20 h before the end of the macrophage culture,3H-thymidine (2.0 Ci/mmol; PerkinElmer, MA, USA) was added to the medium in the wells. When the culture was finished, the cells were adsorbed on 0.45 μm membrane filters (Advantech Japan, Tokyo, Japan), washed with distilled water, and then dried. The filters were transferred to vials filled with liquid scintillator cocktail, and the radioactivity was measured with a liquid scintillation counter (LSC-6100, Hitachi Aloka Medical, Tokyo, Japan). Results are given as D.P.M. ± SD of triplicate samples.


## 2.9. Macrophage Phagocytic Assay

The procedure of macrophage phagocytic assay was modified as previous report [20]. Mouse macrophage (2 × 106 cells in 100 μL) andS. pneumoniae(104 CFU in 100 μL) that was opsonized with mouse serum for 30 min were incubated at 37°C for 1 h with shaking on a rotator in 5% CO2 atmosphere. Then, the aliquots were plated on TSAII sheep blood agar to determine the number of CFU after 0, 30, 60, 90, and 120 min. Data were expressed as bacterial CFU counts at each time.


## 2.10. Cytokine Production Analysis by ELISA

Peritoneal macrophages were cultured in a 24-well (1 × 105/well) plate in RPMI1640 containing 5% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin (GE Healthcare, Chicago, IL, USA) and incubated for 24 h. Then, the medium was collected, and the concentrations of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), IL-6, and monocyte chemotactic protein-1 (MCP-1) were measured using enzyme-linked immunosorbent assay (ELISA) kits (BioLegend, San Diego, CA, USA).


## 2.11. Macrophage Chemotaxis Assay

Chemotaxis of peritoneal macrophages was assayed using 24-well chemotactic chambers (pore size, 5μm; Costar, ME, USA). The Zymosan-activated serum was prepared by adding 10 mg of Zymosan (Sigma) to 1 mL of FBS and incubation at 37°C for 30 min. After incubation, the serum was centrifuged at 1,000 ×g for 10 min and diluted with PBS to give a 20% solution [21]. The upper chamber compartment was loaded with 100 μL mouse peritoneal macrophage suspension (1 × 106 cells). The lower compartment was filled with 900 μL RPMI1640 medium with both 2% FBS and Zymosan-activated serum. Following 24 h incubation at 37°C with 5% CO2 atmosphere, the lower surface of chambers was stained with Giemsa staining, and the number of cells that had migrated to the lower surface of chamber was counted by fluorescence microscope (Eclipse E800, Nikon Instruments Co., Ltd., Tokyo). Data was determined as the mean of six random high-power fields per well. Each assay was performed at least three times. The chemotactic index was the ratio of the number of cells that had migrated in response to Zymosan-activated serum to the unmigrated number of cells as described before [22].


## 2.12. Statistical Analysis

The statistical analysis was conducted using an unpaired Student’st-test for two groups and Tukey’s multiple comparison test for the differences among multiple groups or two-way ANOVA followed by Tukey’s test for time course study of 3 groups. p values less than 0.05 were considered statistically significant.


## 3. Results

### 3.1. Murine Nasal Infection Model

First of all, we tried to assess whether SSHT would provide the host with a prophylactic or therapeutic effect againstS. pneumoniae nasal infection. Four days after nostril infection ofS. pneumoniae, we evaluated the bacterial colony counts in murine nose. In prophylactic protocol, the CFU count in the nose of SSHT-treated mouse (2.9 g/kg bw/day, 20 times the human daily dosage) was significantly lower than that in the nose of SSHT-untreated mouse (p<0.01) (Figure 2(a)). In therapeutic protocol, the CFU count in the nose of SSHT-treated mouse was also significantly lower than that in the nose of SSHT-untreated mouse (p<0.05) (Figure 2(b)); however, the prophylactic administration was more effective than the therapeutic administration in SSHT treatment (Figure 2(c)). In prophylactic protocol, SSHT dose-dependently reduced the colony formation ofS. pneumoniaein the nose of mice, and 10 times the human daily dosage of SSHT exhibited statistically significant decrease (Figure 3). Histopathological analysis showed the destruction of epithelial nasal mucosa inS. pneumoniae-infected mice, and we confirmed the improvement of epithelial nasal mucosal disruption in SSHT-treated mice in prophylactic protocol (Figure 4). Scanning electron microscopic (SEM) analysis demonstrated the extensive destruction of ciliated epithelial cells inS. pneumoniae-infected mice, and incomplete recovery of ciliated epithelial cells was found in SSHT-treated mice in prophylactic protocol (Figure 5).Figure 2
The colonies ofS. pneumoniae in SSHT-treated and untreated murine nose in (a) prophylactic and (b) therapeutic experiments. The nasal fluids were inoculated on TSAII sheep blood agar and incubated for 24 h. (c) Comparison of colony count between SSHT-treated and untreated mice in prophylactic (left) and therapeutic (right) model. Dosage of SSHT was 2.9 g/kg bw/day. Data represent the mean ± SD (n=6). p∗<0.05 and p∗∗<0.01 by Student’st-test. SSHT: Shin’iseihaito extract.
(a)
(b)
(c)Figure 3
Dose-dependent effect of SSHT on nasalS. pneumoniae infection. Each dosage of SSHT was treated in the prophylactic protocol. Data represent the mean ± SD (n=6). p∗<0.05 and p∗∗<0.01 versus untreated group by Tukey’s multiple comparison test. SSHT: Shin’iseihaito extract.Figure 4
Histopathological analysis of nose. Histological analysis represented H&E staining. (a) Control; (b)S. pneumoniae-infected SSHT-untreated mice; (c)S. pneumoniae-infected SSHT-treated mice. SSHT (2.9 g/kg bw/day) was treated in the prophylactic protocol. SSHT: Shin’iseihaito extract.
(a)
(b)
(c)Figure 5
Scanning electron microscopic analysis of nose. (a) Control; (b)S. pneumoniae-infected SSHT-untreated mice; (c)S. pneumoniae-infected SSHT-treated mice. SSHT (2.9 g/kg bw/day) was treated in the prophylactic protocol. SSHT: Shin’iseihaito extract.
(a)
(b)
(c)


### 3.2. Phagocytic Activity of Peritoneal Macrophage

We next focused on macrophage activity, because macrophage plays a major role in phagocytic and chemotactic activity in murine bacterial infection model. To determine whether peritoneal macrophages from SSHT-treated mice showed elevated activities, we performed3H-thymidine uptake analysis. As shown in Figure 6, the uptake of 3H-thymidine into peritoneal macrophages collected from SSHT-treated mice was significantly higher than that from untreated mice (p<0.01). As we inferred that SSHT might activate the peritoneal macrophages, we performed their phagocytic assays onS. pneumoniae. As shown in Figure 7, macrophages from untreated mice killed approximately 90% of theS. pneumoniae inoculum after 30-minute incubation at 37°C, and the macrophages from SSHT-treated mice killed more than 99% ofS. pneumoniae inoculum. We did not find any bacterial colony at 60-minute incubation under SSHT treatment. Significant differences were observed inS. pneumoniaeCFU reduction between SSHT-treated and untreated mice (p<0.01).Figure 6
3H-thymidine-uptake assay in peritoneal macrophage. SSHT (2.9 g/kg bw/day) was gavaged for 4 days, and peritoneal macrophages were collected. Data represent the mean ± SD (n=6). p∗∗<0.01 by Student’st-test. SSHT: Shin’iseihaito extract.Figure 7
Macrophage phagocytosis assay in peritoneal macrophage. Black square, control; open circle, macrophages from untreated mice; close circle, macrophages from SSHT-treated mice. SSHT (2.9 g/kg bw/day) was gavaged for 4 days, and peritoneal macrophages were collected. Data represent the mean ± SD(n=6). p∗∗<0.01 versus untreated group by two-way ANOVA followed by Tukey’s test. SSHT: Shin’iseihaito extract.


### 3.3. Cytokine Expression of Peritoneal Macrophage

We examined the cytokine expression from peritoneal macrophages induced by SSHT treatment. After 24 h incubation of macrophages, the levels of TNF-α, IL-1β, and IL-6 in culture medium of peritoneal macrophages were measured by ELISA. Our results showed that the levels of these cytokines were significantly upregulated in mice treated with SSHT compared with those in untreated mice (p<0.01, resp., Figure 8).Figure 8
Cytokine levels in culture medium of macrophage. TNF-α, IL-1β, and IL-6 were measured by ELISA. SSHT (2.9 g/kg bw/day) was gavaged for 4 days, and peritoneal macrophages were collected. Data represent the mean ± SD (n=6). p∗∗<0.01 by Student’st-test. SSHT: Shin’iseihaito extract.


### 3.4. Effect of Chemotaxis Activity by Peritoneal Macrophage

To determine whether peritoneal macrophages from SSHT-treated mice upregulated the chemotaxis activity, we performed the chemotaxis assays onS. pneumoniae. As shown in Figure 9, the peritoneal macrophages from SSHT-treated mice migrated more than those from untreated mice. We also found significant differences of migrating cell number and chemotaxis index between macrophages from SSHT-treated and untreated mice (p<0.01).Figure 9
Ex vivo macrophage chemotaxis analysis.Chemotaxis of peritoneal macrophages was assayed using 24-well chemotactic chambers. (a) Left panel, migrated macrophages from SSHT-untreated mice; middle panel, migrated macrophages from SSHT-treated mice; right panel, unmigrated macrophage. (b) Comparison of chemotaxis assay between SSHT treatment and control. Left panel, the number of chemotaxis macrophages; right panel, chemotaxis index. SSHT (2.9 g/kg bw/day) was gavaged for 4 days, and peritoneal macrophages were collected. Data shown represent the mean ± SD (n=6).p∗∗<0.01 by Student’st-test. SSHT: Shin’iseihaito extract.
(a)
(b)


### 3.5. Migration Associated Cytokine Expression of Peritoneal Macrophage

Finally, we examined the migration associated cytokine expression from peritoneal macrophage induced by SSHT. After 24 h incubation of macrophage, MCP-1 levels in culture medium of peritoneal macrophage were measured by ELISA (Figure10). The result showed that MCP-1 was significantly upregulated under SSHT treatment (p<0.01).Figure 10
Chemotaxis cytokine production from macrophage. SSHT (2.9 g/kg bw/day) was gavaged for 4 days, and peritoneal macrophages were collected. MCP-1 was measured by ELISA. Data represent the mean ± SD(n=6). p∗∗<0.01 by Student’st-test. SSHT: Shin’iseihaito extract.


## 3.1. Murine Nasal Infection Model

First of all, we tried to assess whether SSHT would provide the host with a prophylactic or therapeutic effect againstS. pneumoniae nasal infection. Four days after nostril infection ofS. pneumoniae, we evaluated the bacterial colony counts in murine nose. In prophylactic protocol, the CFU count in the nose of SSHT-treated mouse (2.9 g/kg bw/day, 20 times the human daily dosage) was significantly lower than that in the nose of SSHT-untreated mouse (p<0.01) (Figure 2(a)). In therapeutic protocol, the CFU count in the nose of SSHT-treated mouse was also significantly lower than that in the nose of SSHT-untreated mouse (p<0.05) (Figure 2(b)); however, the prophylactic administration was more effective than the therapeutic administration in SSHT treatment (Figure 2(c)). In prophylactic protocol, SSHT dose-dependently reduced the colony formation ofS. pneumoniaein the nose of mice, and 10 times the human daily dosage of SSHT exhibited statistically significant decrease (Figure 3). Histopathological analysis showed the destruction of epithelial nasal mucosa inS. pneumoniae-infected mice, and we confirmed the improvement of epithelial nasal mucosal disruption in SSHT-treated mice in prophylactic protocol (Figure 4). Scanning electron microscopic (SEM) analysis demonstrated the extensive destruction of ciliated epithelial cells inS. pneumoniae-infected mice, and incomplete recovery of ciliated epithelial cells was found in SSHT-treated mice in prophylactic protocol (Figure 5).Figure 2
The colonies ofS. pneumoniae in SSHT-treated and untreated murine nose in (a) prophylactic and (b) therapeutic experiments. The nasal fluids were inoculated on TSAII sheep blood agar and incubated for 24 h. (c) Comparison of colony count between SSHT-treated and untreated mice in prophylactic (left) and therapeutic (right) model. Dosage of SSHT was 2.9 g/kg bw/day. Data represent the mean ± SD (n=6). p∗<0.05 and p∗∗<0.01 by Student’st-test. SSHT: Shin’iseihaito extract.
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(c)Figure 3
Dose-dependent effect of SSHT on nasalS. pneumoniae infection. Each dosage of SSHT was treated in the prophylactic protocol. Data represent the mean ± SD (n=6). p∗<0.05 and p∗∗<0.01 versus untreated group by Tukey’s multiple comparison test. SSHT: Shin’iseihaito extract.Figure 4
Histopathological analysis of nose. Histological analysis represented H&E staining. (a) Control; (b)S. pneumoniae-infected SSHT-untreated mice; (c)S. pneumoniae-infected SSHT-treated mice. SSHT (2.9 g/kg bw/day) was treated in the prophylactic protocol. SSHT: Shin’iseihaito extract.
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(c)Figure 5
Scanning electron microscopic analysis of nose. (a) Control; (b)S. pneumoniae-infected SSHT-untreated mice; (c)S. pneumoniae-infected SSHT-treated mice. SSHT (2.9 g/kg bw/day) was treated in the prophylactic protocol. SSHT: Shin’iseihaito extract.
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## 3.2. Phagocytic Activity of Peritoneal Macrophage

We next focused on macrophage activity, because macrophage plays a major role in phagocytic and chemotactic activity in murine bacterial infection model. To determine whether peritoneal macrophages from SSHT-treated mice showed elevated activities, we performed3H-thymidine uptake analysis. As shown in Figure 6, the uptake of 3H-thymidine into peritoneal macrophages collected from SSHT-treated mice was significantly higher than that from untreated mice (p<0.01). As we inferred that SSHT might activate the peritoneal macrophages, we performed their phagocytic assays onS. pneumoniae. As shown in Figure 7, macrophages from untreated mice killed approximately 90% of theS. pneumoniae inoculum after 30-minute incubation at 37°C, and the macrophages from SSHT-treated mice killed more than 99% ofS. pneumoniae inoculum. We did not find any bacterial colony at 60-minute incubation under SSHT treatment. Significant differences were observed inS. pneumoniaeCFU reduction between SSHT-treated and untreated mice (p<0.01).Figure 6
3H-thymidine-uptake assay in peritoneal macrophage. SSHT (2.9 g/kg bw/day) was gavaged for 4 days, and peritoneal macrophages were collected. Data represent the mean ± SD (n=6). p∗∗<0.01 by Student’st-test. SSHT: Shin’iseihaito extract.Figure 7
Macrophage phagocytosis assay in peritoneal macrophage. Black square, control; open circle, macrophages from untreated mice; close circle, macrophages from SSHT-treated mice. SSHT (2.9 g/kg bw/day) was gavaged for 4 days, and peritoneal macrophages were collected. Data represent the mean ± SD(n=6). p∗∗<0.01 versus untreated group by two-way ANOVA followed by Tukey’s test. SSHT: Shin’iseihaito extract.


## 3.3. Cytokine Expression of Peritoneal Macrophage

We examined the cytokine expression from peritoneal macrophages induced by SSHT treatment. After 24 h incubation of macrophages, the levels of TNF-α, IL-1β, and IL-6 in culture medium of peritoneal macrophages were measured by ELISA. Our results showed that the levels of these cytokines were significantly upregulated in mice treated with SSHT compared with those in untreated mice (p<0.01, resp., Figure 8).Figure 8
Cytokine levels in culture medium of macrophage. TNF-α, IL-1β, and IL-6 were measured by ELISA. SSHT (2.9 g/kg bw/day) was gavaged for 4 days, and peritoneal macrophages were collected. Data represent the mean ± SD (n=6). p∗∗<0.01 by Student’st-test. SSHT: Shin’iseihaito extract.


## 3.4. Effect of Chemotaxis Activity by Peritoneal Macrophage

To determine whether peritoneal macrophages from SSHT-treated mice upregulated the chemotaxis activity, we performed the chemotaxis assays onS. pneumoniae. As shown in Figure 9, the peritoneal macrophages from SSHT-treated mice migrated more than those from untreated mice. We also found significant differences of migrating cell number and chemotaxis index between macrophages from SSHT-treated and untreated mice (p<0.01).Figure 9
Ex vivo macrophage chemotaxis analysis.Chemotaxis of peritoneal macrophages was assayed using 24-well chemotactic chambers. (a) Left panel, migrated macrophages from SSHT-untreated mice; middle panel, migrated macrophages from SSHT-treated mice; right panel, unmigrated macrophage. (b) Comparison of chemotaxis assay between SSHT treatment and control. Left panel, the number of chemotaxis macrophages; right panel, chemotaxis index. SSHT (2.9 g/kg bw/day) was gavaged for 4 days, and peritoneal macrophages were collected. Data shown represent the mean ± SD (n=6).p∗∗<0.01 by Student’st-test. SSHT: Shin’iseihaito extract.
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## 3.5. Migration Associated Cytokine Expression of Peritoneal Macrophage

Finally, we examined the migration associated cytokine expression from peritoneal macrophage induced by SSHT. After 24 h incubation of macrophage, MCP-1 levels in culture medium of peritoneal macrophage were measured by ELISA (Figure10). The result showed that MCP-1 was significantly upregulated under SSHT treatment (p<0.01).Figure 10
Chemotaxis cytokine production from macrophage. SSHT (2.9 g/kg bw/day) was gavaged for 4 days, and peritoneal macrophages were collected. MCP-1 was measured by ELISA. Data represent the mean ± SD(n=6). p∗∗<0.01 by Student’st-test. SSHT: Shin’iseihaito extract.


## 4. Discussion

To our knowledge, this is the first experimental study in which SSHT would be effective inS. pneumoniae-caused murine sinusitis model.After nasalS. pneumoniae infection, SSHT-treated mice showed reduction ofS. pneumoniae colonization in murine nose, improvement of destruction of epidermal mucosa, macrophage phagocytic activity, upregulation of peritoneal macrophage producing cytokines including TNF-α, IL-1β, IL-6, and MCP-1, and macrophage chemotaxis activity. These results suggest that SSHT can play an important role in protection againstS. pneumoniae in mouse sinusitis model.At first, we tried to clarify the mechanism of this prophylactic effect of SSHT. Although we revealed the direct in vitro antibacterial effect of SSHT onS. pneumoniae in previous study [14], the protective effect provided by SSHT would not be due to direct antibacterial effect of SSHT, because the administration of SSHT was oral and all constituents of SSHT would not be absorbed from the intestine and appeared at nose as their original form. In addition, we next tried to clarify whether SSHT would provide the protection if given only after bacterial infection. Even though we found the significant recovery of colonization rate in direct SSHT administration only afterS. pneumoniae infection, the therapeutic effect was less than the prophylactic effect in SSHT treatment. Our data imply that SSHT may have potent preventive effect on the infection ofS. pneumoniae and may be useful for both prophylactic and therapeutic administration forS. pneumoniae sinusitis.Our histopathological results showed the improvement of epithelial nasal mucosal disruption in SSHT-treated mouse in comparison to the epithelial nasal mucosa inS. pneumoniae-infected mouse. This is consistent with the previous report demonstrating that experimental sinus infection in the rabbit model leads to marked destruction of ciliated epithelial cells [23].The phagocytic activity of murine peritoneal macrophage was enhanced in the SSHT-treated group. As a result of systemic immunomodulatory action by the orally administered SSHT extract, activation of phagocytic ability of macrophages as a local immunomodulatory effect occurs in the nasal cavity. It is common for macrophages circulating in the blood to aggregate with migratory cytokines to inflamed tissue sites [24]. Peritoneal macrophages can also be considered as a type of systemic macrophages infiltrated from capillaries such as the abdominal wall. Therefore, peritoneal macrophages can be used as functional analysis of macrophages in local areas such as nasal cavities. Macrophages collected from SSHT-treated mice showed significantly higher TNF-α, IL-1β, IL-6, and MCP-1 production than those from untreated mice. In this study, we focused on inflammatory cytokines (TNF-α, IL-1β, and IL-6) and migratory cytokines (MCP-1). Those inflammatory cytokines activate the phagocytosis of macrophage. Migratory cytokine also activates the migration of macrophage. Bacterial infection induces inflammatory cytokines and causes inflammation. After migratory cytokines of leukocytes are induced for phagocytosis of bacteria, leukocytes assemble in the lesion area. Although the relationship between inflammatory cytokine andS. pneumoniae-caused sinusitis has been unclear, previous report showed thatS. pneumoniaeinduced inflammatory cytokine from bronchoalveolar lavage fluid sample in mouse pneumonia model [25, 26]. Another report also revealed thatS. pneumoniae induced MCP-1 in human epithelial cells [27]. Our results were also consistent with previous reports. We suggested that SSHT-induced cytokine production augments bactericidal activity including phagocytic activity which in turn causes bacterial elimination. Our results also demonstrated that SSHT upregulated the chemotaxis activity of macrophages which played important roles to prevent bacterial infection to the host. Our data also potentially postulate that SSHT may be useful in the treatment of multidrug-resistantS. pneumoniae, because SSHT is not per se an antibiotic but it is an immunomodulator. Although SSHT has antibacterial effect againstS. pneumoniae [14], the occurrence of SSHT-resistantS. pneumoniae in future seems to be impossible, and we speculate that SSHT may be efficacious when used against new strains of antibiotic-resistantS. pneumoniaestrain. We also investigated the effect of SSHT against other cps-typeS. pneumoniae isolates and confirmed that SSHT had the biofilm inhibitory effect on those isolates in vitro. The relationship between specific cps-type isolates and effects of SSHT may not appear. Our results imply that SSHT may also affect the host’s immune system with regard toS. pneumoniae infection.We investigated the function of murine peritoneal macrophage by ex vivo analysis. The ex vivo analysis has the great advantage to more closely reflect in vivo conditions compared to the results obtained using cell culture systems. Indeed, this ex vivo method will be valuable in examining the effects of SSHT therapeutic strategies on macrophage, as it might better reflect in vivo conditions compared to the experiments using direct treatment to cultured tissue or cells, where artefacts are often observed.Juzentaihoto, one of Kampo formulae, upregulated the macrophage phagocytosis [28] and enhanced IL-12 production by modulating Toll-like receptor 4 signaling pathways in murine peritoneal exudate macrophages [29]. Ingestion of Kakkonto, another Kampo formula, not only increased the body temperature but also enhanced the phagocytic activity of macrophages, which was an in vivo defence mechanism [30]. Although Kampo medicines have immunomodulating effect such as the activation of macrophage [20], these mechanisms in detail have been unknown. Kampo medicines are generally composed of several crude drug components, and the interaction of them may enhance the effect of drugs. Since Shin’iseihaito does not contain the same crude drug components as Juzentaihoto or Kakkonto, we cannot deduce the relevance of the immune activation effect between these three drugs, and the immunomodulating crude drugs might newly be found among the components of Shin’iseihaito. As further investigation from these perspectives is needed, SSHT may have pronounced immunomodulating effects. And further investigations involving human subjects aboutS. pneumoniae and other bacterial infections using SSHT are also desired.In summary, Shin’iseihaito is significantly effective for the treatment ofS. pneumoniae sinusitis in murine model. We suggest Shin’iseihaito as the therapeutic candidate for novel effective therapy on human sinusitis caused byS. pneumoniae.



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*Source: 4293291-2017-07-20.xml*