# Greener Synthesis, Characterization, and Antimicrobiological Effects ofHelba Silver Nanoparticle-PMMA Nanocomposite

Authors: Manal A. Awad; Awatif A. Hendi; Khalid M. O. Ortashi; Amnah B. Alanazi; Batool A. ALZahrani; Dina A. Soliman
Journal: International Journal of Polymer Science
 (2019)

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## Abstract

Nanocomposites are characterized as a multiphase material where one of the phases has a dimension in the nanoscale. There has been huge enthusiasm for the commercialization of nanocomposites for an assortment of uses including medicinal, electronic, and basic. The general motivation behind this study was on the development of silver nanoparticles, due to the present enthusiasm encompassing these metals due to their exceptional properties which are not quite the same as the relating bulk material. A novel, simple, cost-effective, nontoxic, and environmentally friendly technique was developed for synthesizing silver nanoparticle- (AgNP-) poly(methyl methacrylate) (PMMA) nanocomposite usingTrigonella foenum-graecum (Helba) aqueous extract. UV-visible spectroscopic analysis was carried out to assess the formulation of AgNPs. The particle size distribution of AgNPs was determined by dynamic light scattering (DLS). The average size of green AgNPs was about 83 nm. Images of spherical green nanoparticles were characterized using transmission electron microscopy (TEM). The resultant green AgNPs were added slowly to polymer (PMMA) solution. The AgNPs encapsulated within the polymer chains were characterized by X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). Modification of thermal stabilities of AgNP/PMMA nanocomposites was confirmed using thermogravimetric analysis (TGA). The green AgNP/PMMA nanocomposites showed improved thermal stabilities. The green AgNP/PMMA nanocomposite film proved antimicrobial in water microbiological testing. Thus, the key findings of the work include the use of a safe and simple nanocomposite, which had marked antibacterial activity and potential application in water filtration.


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## Body

## 1. Introduction

Nanomaterials are the most studied materials of the century that gave birth to a new branch of science known as “nanotechnology.” Nanomaterials are prepared from bulk size materials, but the smaller size and shape of these particles differentiate their chemical actions from those of their parent material [1]. The smaller size of nanomaterials helps them to penetrate specific cellular locations, and their additional surface area facilitates increased adsorption and targeted delivery of substances [2]. Nanomaterials exist in volcanic dust, mineral composites, and anthropogenic waste materials like coal combustion, diesel exhaust, and welding fumes (incidental nanomaterials) [3]. Engineered nanomaterials manufactured with nanoscale dimension are generally grouped into four types: carbon, metals, metal oxides, dendrimers, and composites [4].Silver nanoparticles (AgNPs) have unique optical, electrical, and thermal properties and are incorporated into products that range from photovoltaics to biological and chemical sensors. Some examples include conductive inks, pastes, and fillers, which utilize AgNPs for their high electrical conductivity, stability, and low sintering temperature. Additional applications include molecular diagnostics and photonic devices, which take advantage of the novel optical properties of these nanomaterials. An increasingly common application is the use of AgNPs for antimicrobial coatings; textile products, keyboards, wound dressings, and biomedical devices now contain AgNPs that continuously release a low level of silver ions to provide protection against bacteria [5].Polymers are host materials for metal nanoparticles [6]. The polymer acts as surface topping specialist when nanoparticles are implanted in them. The nanocomposites obtained display upgraded optical properties [7]. However, the properties of polymer composites depend on the type of nanoparticles incorporated and their size, shape, concentration, and interaction with the polymer matrix. Poly(methyl methacrylate) (PMMA) is a polymeric glass with a wide range of applications. The use of PMMA offers twofold advantages such as availability of carboxylate functional groups for chemical bonding with metal ions and high solubility of PMMA in solvents like DMF for silver nitrate reduction [8].Owing to their diverse properties of polymer nanocomposites such as unprecedented performance, improved properties compared to the constituent parts, design flexibility, and lower life cycle costs, they have attracted considerable attention. The nanocomposite makes use of organic/inorganic nanoparticles incorporated in polymers that produce new materials with a potential application in catalysis, bioengineering, photonics, electronics, and antibacterial activities [9–11]. AgNPs have been shown to form composites with polymers, such as polyvinyl alcohol, polypyrrole, polyvinylidene fluoride, chitosan, and cellulose. For a silver-polymer nanocomposite, it is important to maintain a controllable size of the nanoparticles in the matrix along with a uniform distribution of the same in the polymer matrix. The synthesis of the silver-polymer nanocomposites includes the mixing of a nanoparticle solution with the polymerization mixture. These silver-polymer nanocomposites can have a wide range of applications such as in biomedicine, textiles, water treatment, food storage containers, home appliances, and medical devices [12, 13].There are various modes of synthesis of nanomaterials which include chemical, physical, and biological. Some chemical and physical methods which have been employed have contributed to environmental contamination, since the chemical procedures involved can generate a large amount of hazardous byproducts. Thus, there is a need to develop new “green” synthesis procedures for nanoparticles which are ecofriendly, safe, and nontoxic procedures to be carried out at low energy and temperature. The biological methods include synthesis of nanomaterials from the extracts of plants, bacteria, and fungal species, among other procedures [14, 15].In this study, stable AgNPs were synthesized by the reduction of silver nitrate withTrigonella foenum-graecum aqueous extract. The AgNPs were characterized by transmission electron microscopy (TEM), UV-Vis spectra, and zetasizer. The AgNP/PMMA nanocomposite was characterized via FTIR and XRD, and its thermal stability was assessed using TGA. In addition, the antimicrobial effect of PMMA nanocomposites was evaluated on tap water. The main objective of the study was to use the AgNPs in PMMA nanocomposites as a biofilter for tap water.


## 2. Materials and Methods

### 2.1. Greener Synthesis of AgNPs

Trigonella foenum-graecum (Helba, 3 g) seeds were purchased from the local market in Riyadh City (Saudi Arabia). The seeds were washed several times to remove the dust on the periphery of the seed and then were dried and soaked in 90 mL boiled distilled water overnight. The extract was passed through Whatmann no. 1 filter paper, and the combined filtrate was immediately used for the preparation of nanoparticles. The resultant aqueous filtrate was treated with aqueous solution of silver nitrate (AgNO3).Silver nitrate (1 mmol/mL, analytical grade, Techno Pharmchem, India) was dissolved in 50 mL distilled water with vigorous stirring at 80°C for 5 min. Then, 5 mL ofHelba extract was added to the silver nitrate solution. The colloidal solution changed in color within an hour, which confirmed the reduction of Ag ions and the formation of greener AgNPs. The change in color of the reaction was noted by visual observation. Then, the green nanoparticle solution was incubated at room temperature until it was used.


### 2.2. Preparation of Green AgNP/PMMA Nanocomposite Film

PMMA (6 g, SABIC Company, Saudi Arabia) was dissolved in 50 mL of N,N-dimethylformamide (DMF, R&M Marketing, Essex, UK) with continuous stirring for 3 hours at 80 C. After that, 3 mL of freshly prepared solution of green AgNPs (previous section) was added with constant stirring at 80°C and 8000 rpm. This mixture was further stirred for 1 h to complete the reaction. The whole previous processes were carried out in a fume hood. A light brown solution was obtained due to the formation of silver colloid. At that point, the solution was cast on a glass plate and DMF was allowed to evaporate at room temperature to create a nanocomposite film. The film was air dried under a fume hood. The film was washed with methanol to remove residual DMF and to promote drying and was removed from the glass plate after drying.


### 2.3. Characterization of Green Nanoparticles and Nanocomposite

X-ray diffraction (XRD) (Bruker D8 Discover) scanning was performed to identify the green AgNP/PMMA nanocomposite and PMMA films. TGA analyses of green AgNP/PMMA nanocomposite and PMMA films were carried out in a thermal system (Mettler Toledo TGA/DSC 1). About 4 mg dried film was used for the TGA experiment. TGA thermograms were obtained in the range 0–800°C under nitrogen flow at a rate of 10°C min-1. Their distinct graphs were plotted with weight (percentage) loss and heat flow against temperature.The size of the synthesized green AgNPs was analyzed through a zetasizer (Nano series, HT Laser, ZEN3600 Molvern Instrument, UK). Transmission electron microscopy (JEM-1011, JEOL, Japan) was employed to characterize the size, shape, and morphology of the formed green synthesized nanoparticles at an accelerating voltage of 100 kV.


### 2.4. Water Microbiological Testing

#### 2.4.1. Plate Count Method

The microbial growth in the treated tap water was assessed using the plate count method. The plate count method relies on bacteria growing a colony on a nutrient medium so that the colony becomes visible to the naked eye and the number of colonies on a plate can be counted. To be effective, the dilution of the original sample must be arranged so that on average colonies of the target bacterium between 30 and 300 are grown. To ensure that an appropriate number of colonies will be generated, several dilutions are normally cultured. This approach is widely utilized for the evaluation of the effectiveness of water treatment by the inactivation of representative microbial contaminants such asE. coli following [16, 17].To treat tap water with the green nanocomposite film, a1×1cm green film was soaked in 50 mL tap water in an Erlenmeyer flask and kept for 48 h.Three different types of media, eosin methylene blue (E.M.B) agar, nutrient agar (N.A) for Gram-negative bacteria such asE. coli, and Muller-Hinton (M.H) agar, have been used to obtain many isolated microorganisms. The water samples evaluated were normal tap water as control (NW) and treated tap water (TW).To prepare 250 g of nutrient agar media, 7 g of the agar media was dissolved in 250 mL of distilled water and autoclaved. To prepare 250 g from MacConkey agar, 12.87 g of the agar medium was dissolved in 250 mL of distilled water and then autoclaved. For Muller-Hinton agar media, 9.5 g of the media was dissolved in 250 mL of distilled water and then autoclave it. After this, 1 mL from both treated and untreated tap water (TW or NW) was added to the assigned petri dishes. Thereafter, the media were thoroughly mixed, autoclaved, and incubated upside down at 37°C for 24-48 hours.


## 2.1. Greener Synthesis of AgNPs

Trigonella foenum-graecum (Helba, 3 g) seeds were purchased from the local market in Riyadh City (Saudi Arabia). The seeds were washed several times to remove the dust on the periphery of the seed and then were dried and soaked in 90 mL boiled distilled water overnight. The extract was passed through Whatmann no. 1 filter paper, and the combined filtrate was immediately used for the preparation of nanoparticles. The resultant aqueous filtrate was treated with aqueous solution of silver nitrate (AgNO3).Silver nitrate (1 mmol/mL, analytical grade, Techno Pharmchem, India) was dissolved in 50 mL distilled water with vigorous stirring at 80°C for 5 min. Then, 5 mL ofHelba extract was added to the silver nitrate solution. The colloidal solution changed in color within an hour, which confirmed the reduction of Ag ions and the formation of greener AgNPs. The change in color of the reaction was noted by visual observation. Then, the green nanoparticle solution was incubated at room temperature until it was used.


## 2.2. Preparation of Green AgNP/PMMA Nanocomposite Film

PMMA (6 g, SABIC Company, Saudi Arabia) was dissolved in 50 mL of N,N-dimethylformamide (DMF, R&M Marketing, Essex, UK) with continuous stirring for 3 hours at 80 C. After that, 3 mL of freshly prepared solution of green AgNPs (previous section) was added with constant stirring at 80°C and 8000 rpm. This mixture was further stirred for 1 h to complete the reaction. The whole previous processes were carried out in a fume hood. A light brown solution was obtained due to the formation of silver colloid. At that point, the solution was cast on a glass plate and DMF was allowed to evaporate at room temperature to create a nanocomposite film. The film was air dried under a fume hood. The film was washed with methanol to remove residual DMF and to promote drying and was removed from the glass plate after drying.


## 2.3. Characterization of Green Nanoparticles and Nanocomposite

X-ray diffraction (XRD) (Bruker D8 Discover) scanning was performed to identify the green AgNP/PMMA nanocomposite and PMMA films. TGA analyses of green AgNP/PMMA nanocomposite and PMMA films were carried out in a thermal system (Mettler Toledo TGA/DSC 1). About 4 mg dried film was used for the TGA experiment. TGA thermograms were obtained in the range 0–800°C under nitrogen flow at a rate of 10°C min-1. Their distinct graphs were plotted with weight (percentage) loss and heat flow against temperature.The size of the synthesized green AgNPs was analyzed through a zetasizer (Nano series, HT Laser, ZEN3600 Molvern Instrument, UK). Transmission electron microscopy (JEM-1011, JEOL, Japan) was employed to characterize the size, shape, and morphology of the formed green synthesized nanoparticles at an accelerating voltage of 100 kV.


## 2.4. Water Microbiological Testing

### 2.4.1. Plate Count Method

The microbial growth in the treated tap water was assessed using the plate count method. The plate count method relies on bacteria growing a colony on a nutrient medium so that the colony becomes visible to the naked eye and the number of colonies on a plate can be counted. To be effective, the dilution of the original sample must be arranged so that on average colonies of the target bacterium between 30 and 300 are grown. To ensure that an appropriate number of colonies will be generated, several dilutions are normally cultured. This approach is widely utilized for the evaluation of the effectiveness of water treatment by the inactivation of representative microbial contaminants such asE. coli following [16, 17].To treat tap water with the green nanocomposite film, a1×1cm green film was soaked in 50 mL tap water in an Erlenmeyer flask and kept for 48 h.Three different types of media, eosin methylene blue (E.M.B) agar, nutrient agar (N.A) for Gram-negative bacteria such asE. coli, and Muller-Hinton (M.H) agar, have been used to obtain many isolated microorganisms. The water samples evaluated were normal tap water as control (NW) and treated tap water (TW).To prepare 250 g of nutrient agar media, 7 g of the agar media was dissolved in 250 mL of distilled water and autoclaved. To prepare 250 g from MacConkey agar, 12.87 g of the agar medium was dissolved in 250 mL of distilled water and then autoclaved. For Muller-Hinton agar media, 9.5 g of the media was dissolved in 250 mL of distilled water and then autoclave it. After this, 1 mL from both treated and untreated tap water (TW or NW) was added to the assigned petri dishes. Thereafter, the media were thoroughly mixed, autoclaved, and incubated upside down at 37°C for 24-48 hours.


## 2.4.1. Plate Count Method

The microbial growth in the treated tap water was assessed using the plate count method. The plate count method relies on bacteria growing a colony on a nutrient medium so that the colony becomes visible to the naked eye and the number of colonies on a plate can be counted. To be effective, the dilution of the original sample must be arranged so that on average colonies of the target bacterium between 30 and 300 are grown. To ensure that an appropriate number of colonies will be generated, several dilutions are normally cultured. This approach is widely utilized for the evaluation of the effectiveness of water treatment by the inactivation of representative microbial contaminants such asE. coli following [16, 17].To treat tap water with the green nanocomposite film, a1×1cm green film was soaked in 50 mL tap water in an Erlenmeyer flask and kept for 48 h.Three different types of media, eosin methylene blue (E.M.B) agar, nutrient agar (N.A) for Gram-negative bacteria such asE. coli, and Muller-Hinton (M.H) agar, have been used to obtain many isolated microorganisms. The water samples evaluated were normal tap water as control (NW) and treated tap water (TW).To prepare 250 g of nutrient agar media, 7 g of the agar media was dissolved in 250 mL of distilled water and autoclaved. To prepare 250 g from MacConkey agar, 12.87 g of the agar medium was dissolved in 250 mL of distilled water and then autoclaved. For Muller-Hinton agar media, 9.5 g of the media was dissolved in 250 mL of distilled water and then autoclave it. After this, 1 mL from both treated and untreated tap water (TW or NW) was added to the assigned petri dishes. Thereafter, the media were thoroughly mixed, autoclaved, and incubated upside down at 37°C for 24-48 hours.


## 3. Results and Discussion

### 3.1. Visual Observation and UV-Vis Spectroscopy Analysis

Noble metals display exceptional optical properties because of surface plasmon resonance (SPR) [18]. The development of AgNPs was first checked for change in color from colorless to brown and UV-Vis spectroscopy. The change in color showed the formation of AgNPs because of the reduction of silver metal particles Ag+ to nanoparticles Ag0 [19]. This color refers to the excitation of SPR. As shown in Figure 1, a characteristic SPR band for AgNPs was observed at around 339 nm.Figure 1
UV-visible absorption spectrum of silver nanoparticles prepared using a greener synthesis process.


### 3.2. TEM and Particle Size Analysis

TEM images presented monodisperse AgNPs with spherical shape, as shown in Figure2(a). The average particle size was determined by dynamic light scattering (DLS) and was found to be 83.01 nm as revealed, in the size distribution graph that showed monodisperse AgNPs (Figure 2(b)). These results agreed with and confirmed the results obtained by TEM. These results are in agreement with Goyal et al. who teach that the size determination studies using DLS revealed the synthesized silver nanoparticles using Trigonella foenum-graecum seed extract size between 95 and 110 nm [20].Figure 2
(a) TEM images of greener silver nanoparticles; the scale bar corresponds to 100 nm. (b) Average particle size distribution curve determined by DLS.
(a)
(b)


### 3.3. X-Ray Diffraction Analysis

The structures of pure PMMA and AgNPs in the polymer matrix were investigated using XRD analysis. Therefore, the XRD patterns of pure PMMA polymer and greener AgNPs/polymer nanocomposites were obtained as shown in Figures3(a) and 3(b).Figure 3
XRD patterns of (a) pure PMMA and (b) greener AgNP/PMMA nanocomposite.
(a)
(b)From Figure3(a), it seems clear that pure PMMA thin film possessed no crystalline structure. Therefore, we can say that it had an amorphous structure. As observed in Figure 3(b), the greener AgNP/PMMA nanocomposites exhibited and confirmed the existence of silver in the polymer matrix. It shows that in all the peaks, the metallic pure silver had spherical structure. Refraction was provided by many peaks at about 2θ=35–70° while the first and second peaks were assigned to the lattice planes (1 1 1) and (2 0 0), respectively [21–23]. In addition to that, the polymer/AgNP composite patterns exhibited a two-phase (crystalline and amorphous) structure. The polymer/AgNP composite exhibited broad reflection and typical amorphous nature of the polymer, as expected, and the typical pattern of the face-centered cubic (FCC) Ag crystalline structure indicated the formation of metallic Ag [24].The XRD pattern of PMMA showed that three broad peaks appeared, which corresponded to a mixture of ordered and disordered structures of the amorphous phase of the polymer [25]. The amorphous halo was caused by the spacing of individual polymer chains. Comparison of the diffraction patterns of PMMA and greener AgNP/PMMA nanocomposite showed that the peaks corresponding to PMMA became broader and seemed to disappear because of small AgNPs embedded in the PMMA chains [26].


### 3.4. FTIR Spectroscopy Analysis

To verify that the resultant composites contained PMMA, we determined the FTIR spectra of pure PMMA and the AgNP/PMMA composite, respectively. The results are shown in Figures4(a) and 4(b). As can be seen in Figure 4(a), the bands between 1550 and 1800 cm-1 in pure PMMA were due to acrylate carboxyl groups or C=O vibrations, which are all typical bands of PMMA. The peak at 3000–3007 cm-1 was assigned to the C–H stretching vibration. For the greener AgNP/PMMA nanocomposite, the appearance of the peak was assigned to C=O vibrations at 1550–1800 cm-1 (Figure 4(b)). From the spectra of Figure 4(a), it can be seen that the band at 1,203 cm-1 originated from the C–O–C group. However, compared to Figure 4(a) for pure PMMA, the peak positions of Figure 4(b) for greener AgNP/PMMA shifted because of strong interaction between PMMA and AgNPs. For the AgNP/PMMA nanocomposite (Figure 4(b)), the peaks at 550–650 cm-1 were from the Ag–C stretching vibration, which further proved the existence and reaction between Ag and long-chain alkyl of PMMA. In addition, shifts in C=O ester carbonyl group, C=C, CH3, and C-C for the AgNP/PMMA nanocomposite thin films were also seen, which indicated the modification of PMMA thin film structures by the AgNPs [23, 27–29].Figure 4
FTIR spectra of (a) pure PMMA and (b) Ag/PMMA nanocomposite.


### 3.5. The Thermal Gravimetric Analysis

TGA estimations were carried out on Ag/PMMA nanocomposite and pure PMMA. The samples of settled weight were heated at the rate 100°C/min from room temperature to 800°C, which was in between the boiling point of the solvent and degradation temperature of the polymer. Figure4 demonstrates two particular phases of the watched weight and underlying weight reduction was figured to be around 20% and 5% for PMMA and Ag/PMMA, respectively, from room temperature to 300°C.The weight reduction up to this temperature was credited to low subatomic weight oligomers, loss of moisture, and leftover solvent. The second weight reduction demonstrated the degradation of pure PMMA (Figure5(a)) at over 360°C, which completely disintegrated at 400°C, while the Ag/PMMA nanocomposite disintegrated at over 800°C (Figure 5(b)). The second significant weight reduction was ascribed to the basic deterioration of the polymer. The weight reductions of Ag/PMMA nanoparticles and pure PMMA are shown in Figures 5(a) and 5(b). The thermogravimetric investigation of the Ag/PMMA nanocomposite indicated a decomposition profile beginning at 400°C and proceeding until over 800°C. This demonstrated that the high thermal stability of the polymer was enhanced by the presence of Ag as a nanofiller [30].Figure 5
(a) TGA thermogram of polymer PMMA. (b) TGA thermogram of greener AgNP/PMMA nanocomposite.


### 3.6. The Microbial Growth Results

In this work, microbiological testing was performed for tap water and treated water with a nanocomposite film utilizing different types of media. The outcomes demonstrated that plates with water treated with the nanocomposite film had no growth of microorganisms unlike plates with ordinary tap water (Figure6). This was because the greener AgNPs in the film in treated water had particularly natural substances like nitrogen and phosphorus, which were fundamental to the response of bacterial metabolism.Figure 6
The results of microbial growth for both (a) untreated tap water and (b) water treated with a nanocomposite film utilizing eosin methylene blue (E.M.B) agar, nutrient agar (N.A), and Muller-Hinton (M.H) agar media.The greener nanocomposite showed phenomenal microorganism activity contingent on the nanoparticle size as this parameter changed the surface area in contact with the bacterial species. The presence of green AgNPs in the nanocomposite clarifies the antimicrobial property found in the synthesized nanocomposite film.The greener nanocomposite demonstrated antibacterial activity depending on nanoparticle size as this parameter changed the surface area in contact with the bacterial species. The presence of green AgNPs in the nanocomposite explained the antimicrobial properties found in the prepared nanocomposite.Morones et al. [31] announced that small sized nanoparticles went through cell membranes, leading to cell malfunction. It can be reasoned that the synthesized greener AgNP/PMM nanocomposite was effective against microbes, considering that nanoparticles were uniformly dispersed in the polymer matrix. Generally, the system of inhibitory impacts of Ag particles on microorganisms is incompletely known. A few investigations have detailed that the positive charge on the Ag particle is pivotal for its antimicrobial activity through electrostatic attraction between the negative charge on the cell membrane of the microorganism and positively charged nanoparticles. The antibacterial mechanism of AgNPs is not clearly defined. There are several theories presented in many literatures. One of these theories is AgNPs can cause damage and leakage of intracellular material by attaching to the cell membrane. The second theory mentioned that the cause of cell death is the formation of free radicals by AgNPs since they have the ability to generate pores in the membrane. The third theory considers that the damages of the integrity and permeability of the membrane are caused by the release of silver ions from nanoparticles and that these ions can react with functional protein molecules and DNA; hence, they interfere with DNA metabolism and replication resulting in cell death [32].Strong antibacterial activity against bacteria has been revealed by nanocomposite film as has been reported in several studies [33] Also, the AgNP nanocomposite antibacterial effects have been reported in numerous studies with different theories; the most probable scenario seems to be that silver ions bind to the bacterial cell membrane and damage it by interfering with membrane receptors and with bacterial electron transport. Another scenario is that the killing effect of the bacteria is due to contact killing [34, 35]. Consequently, this effortless approach for producing greener AgNP/polymer nanocomposite film is valuable in many industrial applications. The technique permits the utilization of a nontoxic, cheap, ecofriendly, bioavailable material [26].


## 3.1. Visual Observation and UV-Vis Spectroscopy Analysis

Noble metals display exceptional optical properties because of surface plasmon resonance (SPR) [18]. The development of AgNPs was first checked for change in color from colorless to brown and UV-Vis spectroscopy. The change in color showed the formation of AgNPs because of the reduction of silver metal particles Ag+ to nanoparticles Ag0 [19]. This color refers to the excitation of SPR. As shown in Figure 1, a characteristic SPR band for AgNPs was observed at around 339 nm.Figure 1
UV-visible absorption spectrum of silver nanoparticles prepared using a greener synthesis process.


## 3.2. TEM and Particle Size Analysis

TEM images presented monodisperse AgNPs with spherical shape, as shown in Figure2(a). The average particle size was determined by dynamic light scattering (DLS) and was found to be 83.01 nm as revealed, in the size distribution graph that showed monodisperse AgNPs (Figure 2(b)). These results agreed with and confirmed the results obtained by TEM. These results are in agreement with Goyal et al. who teach that the size determination studies using DLS revealed the synthesized silver nanoparticles using Trigonella foenum-graecum seed extract size between 95 and 110 nm [20].Figure 2
(a) TEM images of greener silver nanoparticles; the scale bar corresponds to 100 nm. (b) Average particle size distribution curve determined by DLS.
(a)
(b)


## 3.3. X-Ray Diffraction Analysis

The structures of pure PMMA and AgNPs in the polymer matrix were investigated using XRD analysis. Therefore, the XRD patterns of pure PMMA polymer and greener AgNPs/polymer nanocomposites were obtained as shown in Figures3(a) and 3(b).Figure 3
XRD patterns of (a) pure PMMA and (b) greener AgNP/PMMA nanocomposite.
(a)
(b)From Figure3(a), it seems clear that pure PMMA thin film possessed no crystalline structure. Therefore, we can say that it had an amorphous structure. As observed in Figure 3(b), the greener AgNP/PMMA nanocomposites exhibited and confirmed the existence of silver in the polymer matrix. It shows that in all the peaks, the metallic pure silver had spherical structure. Refraction was provided by many peaks at about 2θ=35–70° while the first and second peaks were assigned to the lattice planes (1 1 1) and (2 0 0), respectively [21–23]. In addition to that, the polymer/AgNP composite patterns exhibited a two-phase (crystalline and amorphous) structure. The polymer/AgNP composite exhibited broad reflection and typical amorphous nature of the polymer, as expected, and the typical pattern of the face-centered cubic (FCC) Ag crystalline structure indicated the formation of metallic Ag [24].The XRD pattern of PMMA showed that three broad peaks appeared, which corresponded to a mixture of ordered and disordered structures of the amorphous phase of the polymer [25]. The amorphous halo was caused by the spacing of individual polymer chains. Comparison of the diffraction patterns of PMMA and greener AgNP/PMMA nanocomposite showed that the peaks corresponding to PMMA became broader and seemed to disappear because of small AgNPs embedded in the PMMA chains [26].


## 3.4. FTIR Spectroscopy Analysis

To verify that the resultant composites contained PMMA, we determined the FTIR spectra of pure PMMA and the AgNP/PMMA composite, respectively. The results are shown in Figures4(a) and 4(b). As can be seen in Figure 4(a), the bands between 1550 and 1800 cm-1 in pure PMMA were due to acrylate carboxyl groups or C=O vibrations, which are all typical bands of PMMA. The peak at 3000–3007 cm-1 was assigned to the C–H stretching vibration. For the greener AgNP/PMMA nanocomposite, the appearance of the peak was assigned to C=O vibrations at 1550–1800 cm-1 (Figure 4(b)). From the spectra of Figure 4(a), it can be seen that the band at 1,203 cm-1 originated from the C–O–C group. However, compared to Figure 4(a) for pure PMMA, the peak positions of Figure 4(b) for greener AgNP/PMMA shifted because of strong interaction between PMMA and AgNPs. For the AgNP/PMMA nanocomposite (Figure 4(b)), the peaks at 550–650 cm-1 were from the Ag–C stretching vibration, which further proved the existence and reaction between Ag and long-chain alkyl of PMMA. In addition, shifts in C=O ester carbonyl group, C=C, CH3, and C-C for the AgNP/PMMA nanocomposite thin films were also seen, which indicated the modification of PMMA thin film structures by the AgNPs [23, 27–29].Figure 4
FTIR spectra of (a) pure PMMA and (b) Ag/PMMA nanocomposite.


## 3.5. The Thermal Gravimetric Analysis

TGA estimations were carried out on Ag/PMMA nanocomposite and pure PMMA. The samples of settled weight were heated at the rate 100°C/min from room temperature to 800°C, which was in between the boiling point of the solvent and degradation temperature of the polymer. Figure4 demonstrates two particular phases of the watched weight and underlying weight reduction was figured to be around 20% and 5% for PMMA and Ag/PMMA, respectively, from room temperature to 300°C.The weight reduction up to this temperature was credited to low subatomic weight oligomers, loss of moisture, and leftover solvent. The second weight reduction demonstrated the degradation of pure PMMA (Figure5(a)) at over 360°C, which completely disintegrated at 400°C, while the Ag/PMMA nanocomposite disintegrated at over 800°C (Figure 5(b)). The second significant weight reduction was ascribed to the basic deterioration of the polymer. The weight reductions of Ag/PMMA nanoparticles and pure PMMA are shown in Figures 5(a) and 5(b). The thermogravimetric investigation of the Ag/PMMA nanocomposite indicated a decomposition profile beginning at 400°C and proceeding until over 800°C. This demonstrated that the high thermal stability of the polymer was enhanced by the presence of Ag as a nanofiller [30].Figure 5
(a) TGA thermogram of polymer PMMA. (b) TGA thermogram of greener AgNP/PMMA nanocomposite.


## 3.6. The Microbial Growth Results

In this work, microbiological testing was performed for tap water and treated water with a nanocomposite film utilizing different types of media. The outcomes demonstrated that plates with water treated with the nanocomposite film had no growth of microorganisms unlike plates with ordinary tap water (Figure6). This was because the greener AgNPs in the film in treated water had particularly natural substances like nitrogen and phosphorus, which were fundamental to the response of bacterial metabolism.Figure 6
The results of microbial growth for both (a) untreated tap water and (b) water treated with a nanocomposite film utilizing eosin methylene blue (E.M.B) agar, nutrient agar (N.A), and Muller-Hinton (M.H) agar media.The greener nanocomposite showed phenomenal microorganism activity contingent on the nanoparticle size as this parameter changed the surface area in contact with the bacterial species. The presence of green AgNPs in the nanocomposite clarifies the antimicrobial property found in the synthesized nanocomposite film.The greener nanocomposite demonstrated antibacterial activity depending on nanoparticle size as this parameter changed the surface area in contact with the bacterial species. The presence of green AgNPs in the nanocomposite explained the antimicrobial properties found in the prepared nanocomposite.Morones et al. [31] announced that small sized nanoparticles went through cell membranes, leading to cell malfunction. It can be reasoned that the synthesized greener AgNP/PMM nanocomposite was effective against microbes, considering that nanoparticles were uniformly dispersed in the polymer matrix. Generally, the system of inhibitory impacts of Ag particles on microorganisms is incompletely known. A few investigations have detailed that the positive charge on the Ag particle is pivotal for its antimicrobial activity through electrostatic attraction between the negative charge on the cell membrane of the microorganism and positively charged nanoparticles. The antibacterial mechanism of AgNPs is not clearly defined. There are several theories presented in many literatures. One of these theories is AgNPs can cause damage and leakage of intracellular material by attaching to the cell membrane. The second theory mentioned that the cause of cell death is the formation of free radicals by AgNPs since they have the ability to generate pores in the membrane. The third theory considers that the damages of the integrity and permeability of the membrane are caused by the release of silver ions from nanoparticles and that these ions can react with functional protein molecules and DNA; hence, they interfere with DNA metabolism and replication resulting in cell death [32].Strong antibacterial activity against bacteria has been revealed by nanocomposite film as has been reported in several studies [33] Also, the AgNP nanocomposite antibacterial effects have been reported in numerous studies with different theories; the most probable scenario seems to be that silver ions bind to the bacterial cell membrane and damage it by interfering with membrane receptors and with bacterial electron transport. Another scenario is that the killing effect of the bacteria is due to contact killing [34, 35]. Consequently, this effortless approach for producing greener AgNP/polymer nanocomposite film is valuable in many industrial applications. The technique permits the utilization of a nontoxic, cheap, ecofriendly, bioavailable material [26].


## 4. Conclusions

The introduced work demonstrated the fast greener synthesis of AgNPs utilizingTrigonella foenum-graecum seeds and their composite with the PMMA polymer. The technique here is nontoxic, ecologically cordial, and straightforward and involves minimal effort and has no lethal chemicals. The formation of greener AgNPs was determined by TEM and UV-Vis spectroscopy, where surface plasmon absorption maxima can be seen at 339 nm in the UV-Vis range. Zetasizer demonstrated the average size of the resulting nanoparticles to be 83.01 nm. The nanocomposite was characterized utilizing FTIR spectroscopy and XRD techniques. TGA was used to explore the thermal stability and interfacial connection between AgNPs and the polymer matrix. TGA demonstrated that AgNP/PMMA nanocomposite has higher thermal stability than that of the PMMA polymer. The nanocomposite films showed significant antibacterial activity bacteria that exhibited no growth of microbes in treated water. This guarantees a promising potential utilization of the nanocomposite in water decontamination, channels, and water stockpiling, in addition to water and wastewater treatment.



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*Source: 4379507-2019-04-21.xml*