# Sepsis-Related

Authors: Sylvia Maria Porto Pereira; Maria Helena Cabral de Almeida Cardoso; Ana Lucia Figuexeds; Haroldo Mattos; Ronaldo Rozembaum; Vanessa Isidoro Ferreira; Maria Antonieta Portinho; Ana Cristina Gonçalves; Elaine Sobral da Costa
Journal: International Journal of Pediatrics
 (2009)

---

## Abstract

The aim of this study
is to identify risk factors for sepsis-related
mortality in low birth weight
(<1500 g) infants. We performed
retrospective cohort study to investigate risk
factors for sepsis-related mortality in all
neonates birth weight <1500 g
admitted to Level III neonatal intensive care
unit, Brazil, April 2001/September 2004. Of the
203 cases, 71 (35%) had sepsis. Of those,
gram-positive was identified in 52/87 blood
cultures (59.8%), the most commonCoagulase-negative
Staphylococcus (31/87; 35.5%).
Gram-negative was present in 29 of the 87
positive blood cultures (33.3%), with
Pseudomonas aeruginosa (8/87;
9.1%), the most frequent agent. Overall 21
of 71 infants with sepsis (29.6%) died. Risk
factors for sepsis-related mortality were
gestational age ≤28 weeks, birth weight
≤1000 g (9.6 times more often
than birth weight >1000 g),
five-minute Apgar ≤7, gram-negative
sepsis, mechanical ventilation (6.7 times higher
than no use), and intravascular catheter. Sepsis-related mortality was due, mainly, to
Pseudomonas aeruginosa; birth
weight ≤1000 g and mechanical
ventilation were strong sepsis-related mortality
predictors.


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## Body

## 1. Introduction

Neonatal sepsis is a frequent complication of very low birth weight (VLBW) infants and it is an important cause of neonatal morbidity and mortality [1, 2]. VLBW infants develop 2.7 times more sepsis than other infants since their immune system and skin barrier are immature and they are exposed to many invasive diagnostic and therapeutic procedures [3]. Morbidity and mortality in a neonatal intensive care unit (NICU) can be reduced by knowledge of the epidemiology of the microbiology, infection rate profile and antibiotic sensitivity, and by introducing practices that are based on clinical evidence [4, 5]. Special attention must be given to infection by Pseudomonas aeruginosa  due to a high mortality rate [6]. The aim of this study is to identify the risk factors involved in the mortality caused by sepsis of a VLBW infants population with sepsis, in particular, the role of Pseudomonas aeruginosa, a very aggressive pathogen. The infants were hospitalized in the NICU of a high-risk maternity in a tertiary-level public hospital specialized in caring for highly complex patients. Secondary objectives are to determine the frequency and distribution of the pathogens that cause infection and to describe the characteristics of this population.


## 2. Methods

### 2.1. Patients

All VLBW infants were born at the maternity of Servidores do Estado Hospital (SHE), Rio de Janeiro, RJ, Brazil and admitted in the NICU of the same hospital, just after birth, between 1 April, 2001 and 30 September, 2004 meeting the following criteria: birth weight (BW)<1500 g, clinical evidence of systemic infection and positive blood culture result for bacterium or yeast on one or more blood cultures obtained at any time while infants were inpatients in the NICU. Infants died of nonsepsis causes, with lethal congenital malformations and chromosomal abnormalities were excluded.


### 2.2. Chart Review

This retrospective cohort study collected data from the medical records of the selected VLBW infants using an investigation protocol. Relevant informations includedAntenatal and Intrapartum History: gestational age (GA) based on the date of the last menstruation, uterus fundus measurement, and ultrasonography performed until 12 weeks of GA; time elapsed between rupture of membranes and birth; mode of birth; Apgar score at the first, fifth, and tenth minute of life; gender; BW; BW and GA relationship according to Usher and McLean’s growth curves [7]. Events  Related to Infection Episodes: incidence of sepsis, defined by the presence of clinical signs and symptoms as apnea, gastrointestinal problems, increased need for oxygen or ventilatory support, and lethargy/hypotonic, and by one or more positive blood cultures for bacterium or yeast obtained at any time during the infant’s stay at the NICU. Any organism, including Coagulase-negative Staphylococcus  (CONS) was considered the caused agent of sepsis and not a contaminant if the criteria of the Vermont Oxford Network Database were present: clinical signs of sepsis, positive blood culture for CONS, and intravenous antibacterial therapy for at least five days after obtaining blood culture or until death, in case it occurs within five days after obtaining blood culture [8]. Whenever CONS and another pathogen were identified in the same blood culture, only the other pathogen was recorded in the database, CONS was considered contaminant and this agent was discharged; age at the time of onset of sepsis considered to be the day on which the first blood culture for the event was positive: early-onset sepsis (EOS) defined as infection occurring before or at 72 hours of life and late-onset sepsis (LOS), after 72 hours of life [9];  organisms cultured and associated with death from sepsis; Neonatal Comorbidities: perinatal asphyxia defined as the presence of five-minute Apgar score below six and signs of encephalopathy as lethargy/stupor, hypotonia, and abnormal reflexes [10]; respiratory distress syndrome (RDS) defined as the need for oxigenotherapy, clinical features of RDS, and ventilation support and abnormal thorax radiograph in the first 24 hours of life [3]; necrotizing enterocolitis (NEC) classified according to the system of Bell et al. [11];  temperature instability, defined as temperature <36.5°C or >37.5°C or a variation of >1°C in a period of 24 hours and blood changes such as neutropenia, defined by a neutrophil count <1.5×103/L and thrombocytopenia, defined as a platelet count <80×103/L [11]. Therapeutic Interventions: use and length of use of intravascular catheter, defined as a peripherally inserted central catheter or by a vascular dissection and arterial or venous umbilical catheter, inserted before the onset of sepsis; use and length of use of mechanical ventilation and of total parenteral nutrition (TPN); time when enteral feeding was initiated and length of stay in the NICU. Clinical Outcome: neonatal death was considered related to infection when clinical signs and symptoms of sepsis  evolved to signs and symptoms of irreversible septic shock as systemic hypotension, acidemia, anuria, and  hypoxia; and/or signs and symptoms of irreversible disseminated intravascular coagulation syndrome as thrombocytopenia, major bleeding episodes as pulmonary hemorrhage, and, ultimately, progressing to death.


### 2.3. Statistical Analysis

Bivariate analysis was used to evaluate the association between potential risk factors and sepsis-related mortality. For statistical inference, the proportions were compared with the chi-square test (χ2) or with Fisher’s exact test with Yates correction when the expected value in any cell of a 2×2 table is <5. P-value of  .05 or less was considered significant. The cut-off for continuous data was obtained with the Receiver Operator Characteristics (ROC) curve. The cut-off was determined for the value with the highest accuracy from the area of the ROC curve, it means, to the highest area under the ROC curve. The odd ratios (ORs) were calculated with confidence intervals of 95% (CI95) for each risk factor. A stepwise backward unconditional multivariate logistic regression was performed to control for confounding and to assess the independence of the identified risk factors. All variables with a P-value of  .05 or less on bivariate analysis were included in the initial model. ORs and CI95 were calculated. The final model included the statistically significant variables in unconditional logistic regression analyzed by P-value of the test Walds. Statistical analysis was performed using the software Epi Info version 3.3.2, SPSS version 12, and Epi Data version 3.1.The study was reviewed and approved by the local Research Ethics Committee (no. 000197).


## 2.1. Patients

All VLBW infants were born at the maternity of Servidores do Estado Hospital (SHE), Rio de Janeiro, RJ, Brazil and admitted in the NICU of the same hospital, just after birth, between 1 April, 2001 and 30 September, 2004 meeting the following criteria: birth weight (BW)<1500 g, clinical evidence of systemic infection and positive blood culture result for bacterium or yeast on one or more blood cultures obtained at any time while infants were inpatients in the NICU. Infants died of nonsepsis causes, with lethal congenital malformations and chromosomal abnormalities were excluded.


## 2.2. Chart Review

This retrospective cohort study collected data from the medical records of the selected VLBW infants using an investigation protocol. Relevant informations includedAntenatal and Intrapartum History: gestational age (GA) based on the date of the last menstruation, uterus fundus measurement, and ultrasonography performed until 12 weeks of GA; time elapsed between rupture of membranes and birth; mode of birth; Apgar score at the first, fifth, and tenth minute of life; gender; BW; BW and GA relationship according to Usher and McLean’s growth curves [7]. Events  Related to Infection Episodes: incidence of sepsis, defined by the presence of clinical signs and symptoms as apnea, gastrointestinal problems, increased need for oxygen or ventilatory support, and lethargy/hypotonic, and by one or more positive blood cultures for bacterium or yeast obtained at any time during the infant’s stay at the NICU. Any organism, including Coagulase-negative Staphylococcus  (CONS) was considered the caused agent of sepsis and not a contaminant if the criteria of the Vermont Oxford Network Database were present: clinical signs of sepsis, positive blood culture for CONS, and intravenous antibacterial therapy for at least five days after obtaining blood culture or until death, in case it occurs within five days after obtaining blood culture [8]. Whenever CONS and another pathogen were identified in the same blood culture, only the other pathogen was recorded in the database, CONS was considered contaminant and this agent was discharged; age at the time of onset of sepsis considered to be the day on which the first blood culture for the event was positive: early-onset sepsis (EOS) defined as infection occurring before or at 72 hours of life and late-onset sepsis (LOS), after 72 hours of life [9];  organisms cultured and associated with death from sepsis; Neonatal Comorbidities: perinatal asphyxia defined as the presence of five-minute Apgar score below six and signs of encephalopathy as lethargy/stupor, hypotonia, and abnormal reflexes [10]; respiratory distress syndrome (RDS) defined as the need for oxigenotherapy, clinical features of RDS, and ventilation support and abnormal thorax radiograph in the first 24 hours of life [3]; necrotizing enterocolitis (NEC) classified according to the system of Bell et al. [11];  temperature instability, defined as temperature <36.5°C or >37.5°C or a variation of >1°C in a period of 24 hours and blood changes such as neutropenia, defined by a neutrophil count <1.5×103/L and thrombocytopenia, defined as a platelet count <80×103/L [11]. Therapeutic Interventions: use and length of use of intravascular catheter, defined as a peripherally inserted central catheter or by a vascular dissection and arterial or venous umbilical catheter, inserted before the onset of sepsis; use and length of use of mechanical ventilation and of total parenteral nutrition (TPN); time when enteral feeding was initiated and length of stay in the NICU. Clinical Outcome: neonatal death was considered related to infection when clinical signs and symptoms of sepsis  evolved to signs and symptoms of irreversible septic shock as systemic hypotension, acidemia, anuria, and  hypoxia; and/or signs and symptoms of irreversible disseminated intravascular coagulation syndrome as thrombocytopenia, major bleeding episodes as pulmonary hemorrhage, and, ultimately, progressing to death.


## 2.3. Statistical Analysis

Bivariate analysis was used to evaluate the association between potential risk factors and sepsis-related mortality. For statistical inference, the proportions were compared with the chi-square test (χ2) or with Fisher’s exact test with Yates correction when the expected value in any cell of a 2×2 table is <5. P-value of  .05 or less was considered significant. The cut-off for continuous data was obtained with the Receiver Operator Characteristics (ROC) curve. The cut-off was determined for the value with the highest accuracy from the area of the ROC curve, it means, to the highest area under the ROC curve. The odd ratios (ORs) were calculated with confidence intervals of 95% (CI95) for each risk factor. A stepwise backward unconditional multivariate logistic regression was performed to control for confounding and to assess the independence of the identified risk factors. All variables with a P-value of  .05 or less on bivariate analysis were included in the initial model. ORs and CI95 were calculated. The final model included the statistically significant variables in unconditional logistic regression analyzed by P-value of the test Walds. Statistical analysis was performed using the software Epi Info version 3.3.2, SPSS version 12, and Epi Data version 3.1.The study was reviewed and approved by the local Research Ethics Committee (no. 000197).


## 3. Results

### 3.1. Incidence of Infection

Between 1 April, 2001 and 30 September, 2004, 203 VLBW infants were admitted to the NICU of SEH, RJ. During their stay in the NICU, 71/203 (35%) had one or more episodes of sepsis. For most VLBW infants, 61/71 (86%), sepsis onset occurred after 72 hours of life. Most of the VLBW infants, 55/71 (77.5%) had one episode; 11/71 (15.5%) two; 4/71 (5.6%) three; 1/71 (1.4%) four, totalling 87 episodes. A great number of the infected neonates, 30/71 (42.3%) were BW≤ 1000 g, classified as extreme low birth weight (ELBW) infants. The mean VLBW infant’s age for all sepsis events was 12.9 (12.1) days, being 9.8 (9.3) for the first and 26 (15.6) for the second episode.


### 3.2. Pathogen Distribution

The majority of infection events, either EOS or LOS, were due to gram-positive organisms, 52/87 (59.8%). CONS was the most common pathogen of all events (31/87; 35.5%) and among gram-positive events (31/52; 59.6%). Gram-negative organisms account for 29/87 (33.3%) episodes, all LOS.P. aeruginosa  was the most common gram-negative pathogen (8/87; 9.1%), followed by Klebsiella sp. and Acinetobacter sp. Yeasts were found in 6/87 (6.9%) episodes and Candida albicans  was always the causing pathogen. The distribution of the organisms is shown in Table 1.Table 1
Number (%) of episodes of total sepsis, EOS and LOS  in 71 VLBW infants testing positive for each organism,Servidores Estado Hospital, April 2001/September 2004.
ORGANISMTotal SepsisEOSLOSN%n%n%Gram-positive organisms5259.81011.64248.2Coagulase-negative Staphylococcus3135.5782427.5Methicillin-sensible S.  aureus1618.311.21517.1Methicillin-resistant S.  aureus11.2——11.2Enterococcus22.4——22.4Streptococcus agalactiae (B)11.211.2——Listeria monocytogenes11.211.2——Gram-negative organisms2933.3——2933.3Pseudomonas aeruginosa89.1——89.1Klebsiella pneumoniae sp66.9——66.9Acinetobacter calcoaceticus66.9——66.9Unidentified gram-negative33.4——33.4Serratia  marcescens33.4——33.4Enterobacter cloacae22.4——22.4Escherichia coli11.2——11.2Fungi66.9——66.9Candida albicans66.9——66.9Total871001011.67788.4EOS: early-onset-sepsis.LOS: late-onset-sepsis.


### 3.3. Antenatal, Intrapartum, and Neonatal History

Antenatal and intrapartum history, neonatal comorbidities, and therapeutic interventions were reviewed. The continuous data are presented as means and standard deviations and the categorical data are presented as percentages (Table2).Table 2
Risk factors for sepsis-related mortality in 71 VLBW infants with sepsis,Servidores EstadoHospital, April 2001/September 2004.
Risk factors: antenatal and intrapartum history,neonatal comorbidities, therapeutic interventionsContinuous variablesn%Rupture of membranes  at birth5577.5<12 hours before birth34.2Caesarian section5374.6Males3650.7Birth weight≤ 1000 g3042.3Small for gestational age4563.4Perinatal asphyxia3752.1Respiratory distress syndrome3954.9Necrotizing enterocolitis1216.9Temperature instability1216.9Neutropenia1419.7Thrombocytopenia4461.9Intravascular catheter4766.2Total Parenteral nutrition6490.1Mechanical ventilation4259.2Enteral feeding onset > 72 hours1825.3Categorical variablesMeanStandard deviationGestational age   (weeks)312.51-minute Apgar score6.22.15-minute Apgar score8.11.410-minute Apgar score7.22Birth weight  (grams)1054232Lenght of intravascular catheter (days)10.378.7Lenght of total parenteral nutrition (days)9.127.52Lenght of mechanical ventilation (days)6.979.46Length of stay (days)53.835


### 3.4. Sepsis-Related Mortality

Overall 21 of the 71 VLBW infants with sepsis (29.6%) died, being 2/10 (20%) from EOS, and 19/61 (32.7%) from LOS.There were significant differences in death rates from sepsis, depending on the organisms isolated from the last positive culture (Table3). Episodes of sepsis with gram-negative organisms were more likely to result in death than episodes with gram-positive or fungus (13/29; 44.8% versus 7/52; 13.4% versus 1/6; 16.7%; P<.05). Within this high-risk group, VLBW infants with P.  aeruginosa  sepsis were most likely to die (6/8; 75% of infants with P.  aeruginosa  infections died; P<.05).Table 3
Number (%) of organisms related to total sepsis, EOS and LOS in 21VLBW infants who died of sepsis,Servidores do Estado Hospital, April 2001/September 2004.
Sepsis-related mortalityOrganismTotal sepsisEOSLOSNn%n%n%Gram-positive organism52713.411.9611.5Coagulase-negative staphylococcus31412.913.239.6Staphylococcus aureus16212.9––-––-212.5Staphylococcus MRSA11100––-––-1100Others4––-––-––-––-––-––-Gram-negative organism291344.813.41241.4Pseudomonas aeruginosa8675––-675Klebsiella pneumoniae sp6466.7––-––-466.7Serratia3266.7133.3133.3Escherichia coli111001100Others11––-––-––-––-––-––-Fungi6116.7––-––-116.7Candida albicans6116.7––-––-116.7Total87*21**219*Total episodes sepsis-causing organisms**Organisms that caused the last sepsis episode right before death of the 21 VLBW infantsEOS: early-onset-sepsis.LOS: late-onset-sepsis.ELBW infants (BW≤ 1000 g) were significantly more likely to die than those with BW  > 1000 g (17/30; 56.7% versus 4/41; 9.7%;  P<.05). The mean GA, BW, and five-minute Apgar score of VLBW infants who died were lower than that of those who survived (29.4 versus 32 weeks; 875 versus 1130 g; 7.4 versus 8.4; P<.05 each one).VLBW infants submitted to intravascular catheter and mechanical ventilation were significantly more likely to die than those not submitted to these procedures (19/47; 40.4% versus 2/24; 8.3%; and 19/42; 45.26% versus 2/29; 6.9%;P<.05 each one). Enteral feeding onset before or at 72 hours of life and length of stay were highly significant predictors of survival (P<.05). The bivariate analysis is shown in Table 4.Table 4
Risk factors associated with sepsis-related mortality and sepsis relate survival of 71 VLBW infants with sepsis,Servidores Estado  Hospital, April 2001/September 2004.
Risk factorORCI 95PSepsis-related mortalityGestational age≤ 28 weeks14.672.12–116.07.000Birth weight≤ 1000 g10.932.76–47.15.0005th minute Apgar≤ 75.410.75–47.61.064 (Fisher)Gram-negative organisms2.911.14–7.45.023Use of mechanical ventilation11.052.34–53.04.003Use of intravascular catheter3.111.08–8.93.003Sepsis-related survivalEnteral feeding onset≤ 72 hours0.100.00–0.79.022OR = odds ratioIC 95 = 95% confidence interval.


### 3.5. Logistic Regression

The predictive model based on a stepwise backward unconditional multivariate logistic regression for 71 VLBW infants showed that BW independently contributed most to the dependent variable death, with a cut-off point of 1000 g. BW ≤1000 g infants presented a sepsis-related mortality rate 9.6 times higher than BW> 1000 g infants (P<.05). The use of mechanical ventilation presented a clinically significant risk (sepsis-related mortality rate 6.7 times higher) but the statistical significance was marginal (P=.05). Length of stay was statistically significant with a negative coefficient, that is, the risk of death from sepsis decreased as length of stay increased (P<.05). The logistic regression analysis is shown in Table 5.Table 5
Risk factors  for mortality and for survival in the 71VLBW infants with sepsis, identified by  predictive model based on a backward step-by-step unconditional multivariate logistic regression.
Risk FactorORCI95PStep  1(a)Length of stay0.9330.900–0.967.000Step  2(b)Length of stay>33 days0.9380.905–0.972.000Birth weight≤1000 g13.2322.603–67.249.002Step  3(c)Mechanical ventilation6.7261.043–43.363.045Length of stay>28 days0.9440.912–0.978.001Birth weight≤1000 g9.6001.702–54.156.010Odds ratio (OR) for the probability of death by sepsisIC95 = 95% confidence interval.(a) variable used in step  1       length of stay>28 days(b) variable used in step  2       birth weight≤1000 g.(c) variable used in step  3       mechanical ventilationR2=0.685.


## 3.1. Incidence of Infection

Between 1 April, 2001 and 30 September, 2004, 203 VLBW infants were admitted to the NICU of SEH, RJ. During their stay in the NICU, 71/203 (35%) had one or more episodes of sepsis. For most VLBW infants, 61/71 (86%), sepsis onset occurred after 72 hours of life. Most of the VLBW infants, 55/71 (77.5%) had one episode; 11/71 (15.5%) two; 4/71 (5.6%) three; 1/71 (1.4%) four, totalling 87 episodes. A great number of the infected neonates, 30/71 (42.3%) were BW≤ 1000 g, classified as extreme low birth weight (ELBW) infants. The mean VLBW infant’s age for all sepsis events was 12.9 (12.1) days, being 9.8 (9.3) for the first and 26 (15.6) for the second episode.


## 3.2. Pathogen Distribution

The majority of infection events, either EOS or LOS, were due to gram-positive organisms, 52/87 (59.8%). CONS was the most common pathogen of all events (31/87; 35.5%) and among gram-positive events (31/52; 59.6%). Gram-negative organisms account for 29/87 (33.3%) episodes, all LOS.P. aeruginosa  was the most common gram-negative pathogen (8/87; 9.1%), followed by Klebsiella sp. and Acinetobacter sp. Yeasts were found in 6/87 (6.9%) episodes and Candida albicans  was always the causing pathogen. The distribution of the organisms is shown in Table 1.Table 1
Number (%) of episodes of total sepsis, EOS and LOS  in 71 VLBW infants testing positive for each organism,Servidores Estado Hospital, April 2001/September 2004.
ORGANISMTotal SepsisEOSLOSN%n%n%Gram-positive organisms5259.81011.64248.2Coagulase-negative Staphylococcus3135.5782427.5Methicillin-sensible S.  aureus1618.311.21517.1Methicillin-resistant S.  aureus11.2——11.2Enterococcus22.4——22.4Streptococcus agalactiae (B)11.211.2——Listeria monocytogenes11.211.2——Gram-negative organisms2933.3——2933.3Pseudomonas aeruginosa89.1——89.1Klebsiella pneumoniae sp66.9——66.9Acinetobacter calcoaceticus66.9——66.9Unidentified gram-negative33.4——33.4Serratia  marcescens33.4——33.4Enterobacter cloacae22.4——22.4Escherichia coli11.2——11.2Fungi66.9——66.9Candida albicans66.9——66.9Total871001011.67788.4EOS: early-onset-sepsis.LOS: late-onset-sepsis.


## 3.3. Antenatal, Intrapartum, and Neonatal History

Antenatal and intrapartum history, neonatal comorbidities, and therapeutic interventions were reviewed. The continuous data are presented as means and standard deviations and the categorical data are presented as percentages (Table2).Table 2
Risk factors for sepsis-related mortality in 71 VLBW infants with sepsis,Servidores EstadoHospital, April 2001/September 2004.
Risk factors: antenatal and intrapartum history,neonatal comorbidities, therapeutic interventionsContinuous variablesn%Rupture of membranes  at birth5577.5<12 hours before birth34.2Caesarian section5374.6Males3650.7Birth weight≤ 1000 g3042.3Small for gestational age4563.4Perinatal asphyxia3752.1Respiratory distress syndrome3954.9Necrotizing enterocolitis1216.9Temperature instability1216.9Neutropenia1419.7Thrombocytopenia4461.9Intravascular catheter4766.2Total Parenteral nutrition6490.1Mechanical ventilation4259.2Enteral feeding onset > 72 hours1825.3Categorical variablesMeanStandard deviationGestational age   (weeks)312.51-minute Apgar score6.22.15-minute Apgar score8.11.410-minute Apgar score7.22Birth weight  (grams)1054232Lenght of intravascular catheter (days)10.378.7Lenght of total parenteral nutrition (days)9.127.52Lenght of mechanical ventilation (days)6.979.46Length of stay (days)53.835


## 3.4. Sepsis-Related Mortality

Overall 21 of the 71 VLBW infants with sepsis (29.6%) died, being 2/10 (20%) from EOS, and 19/61 (32.7%) from LOS.There were significant differences in death rates from sepsis, depending on the organisms isolated from the last positive culture (Table3). Episodes of sepsis with gram-negative organisms were more likely to result in death than episodes with gram-positive or fungus (13/29; 44.8% versus 7/52; 13.4% versus 1/6; 16.7%; P<.05). Within this high-risk group, VLBW infants with P.  aeruginosa  sepsis were most likely to die (6/8; 75% of infants with P.  aeruginosa  infections died; P<.05).Table 3
Number (%) of organisms related to total sepsis, EOS and LOS in 21VLBW infants who died of sepsis,Servidores do Estado Hospital, April 2001/September 2004.
Sepsis-related mortalityOrganismTotal sepsisEOSLOSNn%n%n%Gram-positive organism52713.411.9611.5Coagulase-negative staphylococcus31412.913.239.6Staphylococcus aureus16212.9––-––-212.5Staphylococcus MRSA11100––-––-1100Others4––-––-––-––-––-––-Gram-negative organism291344.813.41241.4Pseudomonas aeruginosa8675––-675Klebsiella pneumoniae sp6466.7––-––-466.7Serratia3266.7133.3133.3Escherichia coli111001100Others11––-––-––-––-––-––-Fungi6116.7––-––-116.7Candida albicans6116.7––-––-116.7Total87*21**219*Total episodes sepsis-causing organisms**Organisms that caused the last sepsis episode right before death of the 21 VLBW infantsEOS: early-onset-sepsis.LOS: late-onset-sepsis.ELBW infants (BW≤ 1000 g) were significantly more likely to die than those with BW  > 1000 g (17/30; 56.7% versus 4/41; 9.7%;  P<.05). The mean GA, BW, and five-minute Apgar score of VLBW infants who died were lower than that of those who survived (29.4 versus 32 weeks; 875 versus 1130 g; 7.4 versus 8.4; P<.05 each one).VLBW infants submitted to intravascular catheter and mechanical ventilation were significantly more likely to die than those not submitted to these procedures (19/47; 40.4% versus 2/24; 8.3%; and 19/42; 45.26% versus 2/29; 6.9%;P<.05 each one). Enteral feeding onset before or at 72 hours of life and length of stay were highly significant predictors of survival (P<.05). The bivariate analysis is shown in Table 4.Table 4
Risk factors associated with sepsis-related mortality and sepsis relate survival of 71 VLBW infants with sepsis,Servidores Estado  Hospital, April 2001/September 2004.
Risk factorORCI 95PSepsis-related mortalityGestational age≤ 28 weeks14.672.12–116.07.000Birth weight≤ 1000 g10.932.76–47.15.0005th minute Apgar≤ 75.410.75–47.61.064 (Fisher)Gram-negative organisms2.911.14–7.45.023Use of mechanical ventilation11.052.34–53.04.003Use of intravascular catheter3.111.08–8.93.003Sepsis-related survivalEnteral feeding onset≤ 72 hours0.100.00–0.79.022OR = odds ratioIC 95 = 95% confidence interval.


## 3.5. Logistic Regression

The predictive model based on a stepwise backward unconditional multivariate logistic regression for 71 VLBW infants showed that BW independently contributed most to the dependent variable death, with a cut-off point of 1000 g. BW ≤1000 g infants presented a sepsis-related mortality rate 9.6 times higher than BW> 1000 g infants (P<.05). The use of mechanical ventilation presented a clinically significant risk (sepsis-related mortality rate 6.7 times higher) but the statistical significance was marginal (P=.05). Length of stay was statistically significant with a negative coefficient, that is, the risk of death from sepsis decreased as length of stay increased (P<.05). The logistic regression analysis is shown in Table 5.Table 5
Risk factors  for mortality and for survival in the 71VLBW infants with sepsis, identified by  predictive model based on a backward step-by-step unconditional multivariate logistic regression.
Risk FactorORCI95PStep  1(a)Length of stay0.9330.900–0.967.000Step  2(b)Length of stay>33 days0.9380.905–0.972.000Birth weight≤1000 g13.2322.603–67.249.002Step  3(c)Mechanical ventilation6.7261.043–43.363.045Length of stay>28 days0.9440.912–0.978.001Birth weight≤1000 g9.6001.702–54.156.010Odds ratio (OR) for the probability of death by sepsisIC95 = 95% confidence interval.(a) variable used in step  1       length of stay>28 days(b) variable used in step  2       birth weight≤1000 g.(c) variable used in step  3       mechanical ventilationR2=0.685.


## 4. Discussion

In our four-year cohort, sepsis was frequent, 35%, with the highest risk of occurrence in the first two weeks of stay. Even thought was caused mainly by gram-positive organisms, represented by CONS, the mortality was higher when caused by gram-negative organisms, particularlyP. aeruginosa. Neonatal sepsis-related mortality rate was high, 29.6%, in most cases involving ELBW infants, 56.7%, and due to gram-negative organisms, 44.8%, especially P. aeruginosa, 75%. The number of VLBW infants studied was low, however, representative of the whole population of neonates with BW  < 1500 g assisted in a NICU of a reference center for high-risk pregnant women, during the selected period. Being a retrospective study, there was a lack of comprehensive information about risk factors. For this reason, the possible risk factors of sepsis-related mortality were reported under Chart Review and included characteristics of the VLBW infants population and events related to infection episodes.There was a predominance of gram-positive organisms in EOS, especially CONS. This finding differs from the reports of Stoll et al. [9] and Rønnestad et al. [12] that, in the recent years, there was a change in the profile of the pathogens causing EOS in NICU, with increase of gram-negative organisms, especially Escherichia coli. The incidence of EOS due to Group B  Streptococcus  (GBS) was low, a single episode, probably because, in most cases the evaluated VLBW infants were product of the interruption of a high-risk pregnancy by caesarian section with ruptured of amniotic membranes at birth. According to Hickman et al. [13], these two factors contribute to the decrease of the vertical transmission of GBS and the lowest occurrence of EOS due to this agent, as in our study.There was a predominance of gram-positive organisms in LOS, 48.2%, mainly CONS, 27.5%. Costa et al. [14] in Portugal, Khashu [15] in Canada, Sarkar et al. [16] in the United State of America, and Richards et al. [17] in Colombia reported similar findings, what demonstrates that these organisms are prevalent agents of LOS in different countries and continents. P. aeruginosa  was the most frequent agent of LOS, 9.1%, among gram-negative organisms. In contrast to our findings, Afroza [1] and Trotman and Bell [5] reported K. pneumoniae  as the most usual infectious agent among gram-negative organisms in LOS. The reason for the high incidence of P. aeruginosa  in our population must be object of future studies.The sepsis-related mortality rate of VLBW infants was high, 29.6%, when compared to the rates reported in the literature, 17.3% to 21% [5, 6, 17]. VLBW infants with gram-negative sepsis were at the greatest risk of death, 44.8%. P. aeruginosa  was the most aggressive agent of sepsis, responsible for the highest sepsis-related mortality rate, 75%, as reported by studies of Gordon and Isaacs [6].BW≤ 1000 g was identified by logistic regression as the strongest independent sepsis-related mortality predictor. ELBW infants, a great vulnerable population to neonatal complications, presented the highest sepsis-related mortality rate, 56.6%. Afroza [1] related that, among many risk factors for infection, the single most important factor is low birth weight, as we observed. This finding suggests that efforts to reduce neonatal sepsis-related mortality must be turned especially to this population.The use of mechanical ventilation was identified by logistic regression as a strong independent sepsis-related mortality predictor, which is in agreement with Flidel-Rimon et al. [18] and Stoll et al. [19]. This may be caused by the fact that neonates who need ventilation are the sickest patients, but also may be in part related to preventable ventilator-associated infection.Enteral feeding initiated before or at 72 hours of life was a predictor of survival. Our findings suggest the practice of early onset of feeding, and of the discerning indication and maintenance of invasive procedures directed to the treatment of VLBW infants to control sepsis-related mortality in this population.In summary, sepsis-related mortality is very high in low birth weight infants, mainly in extreme low birth infants. This is particularly true for patients with positive blood cultures due toP. aeruginosa. Attention to the control of prematurity, knowledge of the neonatal flora and antibiotic sensitivity profile, introduction of early nutritional support with early onset enteral feeding and efforts to decrease ventilator related events may play a role in decreasing the mortality and morbidity of this very serious disease.



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*Source: 427682-2010-02-21.xml*