# Graphene Oxide Reinforced Polycarbonate Nanocomposite Films with Antibacterial Properties

Authors: R. Mahendran; D. Sridharan; K. Santhakumar; T. A. Selvakumar; P. Rajasekar; J.-H. Jang
Journal: Indian Journal of Materials Science
 (2016)

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## Abstract

The incorporation of carbonaceous nanofillers into polymers can result in significant materials with improved physicochemical properties and novel composite functionalities. In this study, we have fabricated antibacterial, lightweight, transparent, and flexible graphene oxide (GO) reinforced polycarbonate thin films by a facile and low-cost methodology. Solution blending is employed to get a homogeneous mixture of PC-GO composites at various loading of GO, and the thin films are prepared by dry-wet phase inversion technique. Thermal studies and micrographs of the films revealed the incorporation of GO in PC matrix. Microstructure of the thin films showed the homogeneous dispersion of GO at micro- and nanoscales; however, at higher loading of GO (0.7%), significant agglomeration is observed. More importantly, PC-GO composite films exhibited excellent antibacterial activities againstE. coli andS. aureus, owing to the antibacterial nature of GO nanoparticles.


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## Body

## 1. Introduction

Antibacterial nanomaterials possess the ability to inhibit/destroy the growth of the bacteria; hence, they are effectively used in biomedical devices, biomechanics, and tissue engineering applications [1]. Notably, the antibacterial nanomaterials are applied to inhibit bacterial infection in implanted medical devices, since the bacterial infection can cause device failure and tissue destruction [2]. Conventionally, silver nanoparticles are being used as antibacterial materials in biomedical devices; however, they have certain drawbacks such as high cost, scalability, toxicity to the environment, and problems in disposal of the wastes [3]. Recently, graphene and its derivatives have attracted increasing attention in making antibacterial materials owing to their excellent antibacterial activities against bacteria [4, 5]. However, large surface area and strong van der Waals force among the graphene sheets make significant agglomeration in the polymer matrix and the powdery form protects their practical applications [6, 7]. Furthermore, the aromatic nature of the C-C bond of the graphene makes them chemically stable and inert [8]. These drawbacks could be overcome by using functionalized graphenes, particularly graphene oxide (GO), which possesses oxygen functionalities (carboxyl, hydroxyl, and epoxy groups) and is well dispersed in water and some organic solvents [9, 10].Recently, the GOs have been validated as a potential candidate for killing Gram-negative (E. coli) and Gram-positive (S. aureus) bacteria [4, 7, 11–15]. Owing to their excellent antibacterial nature, the reinforcement of the GOs in a polymer matrix not only enhances the antibacterial nature of the pristine polymer but also improves the mechanical, thermal, and chemical stabilities significantly [16, 17]. In addition, the hydrophilic functional groups (hydroxyl, carboxylic, and epoxy groups) of GO could be used to make homogeneous dispersion and proper alignment of GO in the polymer matrix [17]. All of these outstanding traits make the GO an ideal candidate for blending with polymers to improve the antibacterial nature of the host polymers.Polycarbonate (PC), an engineered thermoplastic polymer, possesses excellent temperature resistance, optical transparency, high impact strength, good optical properties, and dimensional stability [18]. Hence, it is widely used in automotive industry, components of aircraft, electronics, data storage, and biomedical devices such as hemodialyzers, drug delivery carriers, blood oxygenators, and arterial filters [19]. However, PCs have certain drawbacks such as less antibacterial activities, stress cracking, low scratch resistance, high sensitivity to abrasive cleaners, and ability to deteriorate in many organic solvents [18, 19]. Generally, the PC’s antibacterial activity is being enhanced by functionalizing reactive groups/blending with other antibacterial materials [20–23].In this study, we report a facile, low-cost, and scalable technique for the fabrication of PC-GO nanocomposites to achieve homogeneous dispersion of GO in the polymer matrix and make strong interactions between PC and GO at the molecular level. In addition, weak hydrogen bonding between the oxygen-containing functional groups of GO and carbonyl groups of the PC could make strong interaction between them. The incorporation of GO in the PC matrix was substantiated by thermal analysis and SEM micrographs. Furthermore, we have investigated the antibacterial activity of PC-GO, PC, and GO againstS. aureusandE. coli. To the best of our knowledge, the antibacterial studies of GO reinforced PC nanocomposite thin films have not yet been reported for tissue engineering applications.


## 2. Materials and Methods

### 2.1. Materials

Bisphenol A polycarbonate (PC) was supplied by GE Plastic Ltd., India, and was dried at 100°C in an oven for 6 h prior to use. Graphene oxide was prepared from graphite flakesvia modified Hummer’s method [24]. Dimethyl formamide (DMF) was purchased from Sigma-Aldrich, India, and used as received and distilled water was used as coagulant.


### 2.2. Fabrication of PC and PC-GO Thin Films

The PC and PC-GO thin films were prepared by dry-wet phase inversion technique [25–27]. Figure 1 shows the schematic representation of the fabrication of PC-GO nanocomposites. Briefly, the PC (20 wt%) was dissolved in DMF and stirred for 5 hrs at 60°C by magnetic stirring to obtain a homogeneous solution. Consequently, the GO was dispersed in DMF by ultrasonication for 2 min, followed by mechanical agitation for 5 hrs. The GO dispersion was mixed with the PC solution and stirred for 7 hrs at 50°C to obtain a homogenous solution. For fabricating thin films, the PC-GO solution was poured on a glass plate and cast by a casting knife (thickness: 50 μm) at room temperature. The nascent film was kept on a hot plate (80°C) to evaporate the solvent for 90 s and dipped in a coagulation (deionized water) bath to leach out the film from the glass plate. The film was placed in a coagulation bath for 3 hrs and then dried in an oven at 80°C for 5 hrs to remove the residual water.Figure 1
Schematic representation for fabrication of PC-GO thin films.


### 2.3. Characterizations

Fourier Transform-Infrared Spectroscopy (FT-IR, Perkin Elmer, Spectrum RX I) was carried out for GO, PC, and PC-GO 3 nanocomposite over the wave number range of 4000–500 cm−1. Thermogravimetric analysis (TGA/SDTA851e, Mettler Toledo) of the GO, PC, and PC-GO 3 nanocomposite was carried out from room temperature to 1000°C at 10°C/min under nitrogen atmosphere, to conclude the incorporation of GO in the PC matrix. The transparency of the pristine PC and PC-GO composites was measured using UV-Vis-NIR spectrophotometer (UV-Vis-NIR, Cary 5000). Raman Spectrometer (model: T64000, HORIBA Jobin Yvon, France) was employed to characterize the GO, PC, and PC-GO 3 samples over the wave number range of 500–3500 cm−1.


### 2.4. Morphological Studies

The surface and cross-sectional microstructures of the PC and PC-GO 1, PC-GO 2, PC-GO 3, and PC-GO 4 nanocomposite films were examined by Field Emission-Scanning Electron Microscopy (FE-SEM, Hitachi, S-4700) to analyze the dispersion and agglomeration of GO in the PC matrix. For the analysis, the thin film was dipped in liquid nitrogen for 20–25 s and then fractured. A thin layer of gold was coated on the thin film’s top surface and cross section using a gold sputter coating device.


### 2.5. Evaluation of Antibacterial Activities

The antibacterial activities of the PC, GO (10 mg, powdery form), and PC-GO 3 nanocomposite were assessed usingE. coli (Gram-negative) andS. aureus(Gram-positive) by Disc Diffusion method (Kirby Bauer test) [28, 29]. For the analysis, the medium was sterilized at 120°C (15 lb/in2). About 30 mL of the Müller Hinton Agar (MHA) medium was transferred aseptically into sterilized Petri plates and kept at 37°C for solidification. The bacterial strains were spread over the Petri plates using L-rod. The powdery forms of GO, PC, and PC-GO films were placed on the media containingE. coli andS. aureus cultures, individually, and incubated for 24 h at 37°C. The inhibition zones of the samples were identified and captured.


## 2.1. Materials

Bisphenol A polycarbonate (PC) was supplied by GE Plastic Ltd., India, and was dried at 100°C in an oven for 6 h prior to use. Graphene oxide was prepared from graphite flakesvia modified Hummer’s method [24]. Dimethyl formamide (DMF) was purchased from Sigma-Aldrich, India, and used as received and distilled water was used as coagulant.


## 2.2. Fabrication of PC and PC-GO Thin Films

The PC and PC-GO thin films were prepared by dry-wet phase inversion technique [25–27]. Figure 1 shows the schematic representation of the fabrication of PC-GO nanocomposites. Briefly, the PC (20 wt%) was dissolved in DMF and stirred for 5 hrs at 60°C by magnetic stirring to obtain a homogeneous solution. Consequently, the GO was dispersed in DMF by ultrasonication for 2 min, followed by mechanical agitation for 5 hrs. The GO dispersion was mixed with the PC solution and stirred for 7 hrs at 50°C to obtain a homogenous solution. For fabricating thin films, the PC-GO solution was poured on a glass plate and cast by a casting knife (thickness: 50 μm) at room temperature. The nascent film was kept on a hot plate (80°C) to evaporate the solvent for 90 s and dipped in a coagulation (deionized water) bath to leach out the film from the glass plate. The film was placed in a coagulation bath for 3 hrs and then dried in an oven at 80°C for 5 hrs to remove the residual water.Figure 1
Schematic representation for fabrication of PC-GO thin films.


## 2.3. Characterizations

Fourier Transform-Infrared Spectroscopy (FT-IR, Perkin Elmer, Spectrum RX I) was carried out for GO, PC, and PC-GO 3 nanocomposite over the wave number range of 4000–500 cm−1. Thermogravimetric analysis (TGA/SDTA851e, Mettler Toledo) of the GO, PC, and PC-GO 3 nanocomposite was carried out from room temperature to 1000°C at 10°C/min under nitrogen atmosphere, to conclude the incorporation of GO in the PC matrix. The transparency of the pristine PC and PC-GO composites was measured using UV-Vis-NIR spectrophotometer (UV-Vis-NIR, Cary 5000). Raman Spectrometer (model: T64000, HORIBA Jobin Yvon, France) was employed to characterize the GO, PC, and PC-GO 3 samples over the wave number range of 500–3500 cm−1.


## 2.4. Morphological Studies

The surface and cross-sectional microstructures of the PC and PC-GO 1, PC-GO 2, PC-GO 3, and PC-GO 4 nanocomposite films were examined by Field Emission-Scanning Electron Microscopy (FE-SEM, Hitachi, S-4700) to analyze the dispersion and agglomeration of GO in the PC matrix. For the analysis, the thin film was dipped in liquid nitrogen for 20–25 s and then fractured. A thin layer of gold was coated on the thin film’s top surface and cross section using a gold sputter coating device.


## 2.5. Evaluation of Antibacterial Activities

The antibacterial activities of the PC, GO (10 mg, powdery form), and PC-GO 3 nanocomposite were assessed usingE. coli (Gram-negative) andS. aureus(Gram-positive) by Disc Diffusion method (Kirby Bauer test) [28, 29]. For the analysis, the medium was sterilized at 120°C (15 lb/in2). About 30 mL of the Müller Hinton Agar (MHA) medium was transferred aseptically into sterilized Petri plates and kept at 37°C for solidification. The bacterial strains were spread over the Petri plates using L-rod. The powdery forms of GO, PC, and PC-GO films were placed on the media containingE. coli andS. aureus cultures, individually, and incubated for 24 h at 37°C. The inhibition zones of the samples were identified and captured.


## 3. Results and Discussion

### 3.1. Transparency of PC and PC-GO Thin Films

Figure2 shows the images of the PC and PC-GO 1 (0.1 wt%), PC-GO 2 (0.3 wt%), PC-GO 3 (0.5 wt%), and PC-GO 4 (0.7 wt%) (for convenience, nanocomposites thin film compositions are denoted by PC-GO 1, PC-GO 2, PC-GO 3, and PC-GO 4, resp.) nanocomposite transparent films, which changed in color from lighter to darker (black color) with respect to the loading of GO in the PC matrix; at higher loading of GO (0.7 wt%), the darkest and more agglomerated film was obtained (Figure 2(e)). This might be due to the visible region absorption of C=C bonds in the GO, owing to the average number of C=C double bonds being increased by increasing the concentration of GO [30]. Table 1 shows the composition of PC and PC-GO 1, PC-GO 2, PC-GO 3, and PC-GO 4 nanocomposites and thickness of the as-prepared thin films. The thicknesses of the PC and PC-GO thin films were clearly measured by using Digimatic Micrometer (Mitutoyo, Series 293 MDC-MX Lite) and the FE-SEM images. The thickness of the PC-GO 1, PC-GO 2, PC-GO 3, and PC-GO 4 thin films minutely increased with respect to the content of GO in the polymer matrix.Table 1
Composition of PC and PC-GO 1, PC-GO 2, PC-GO 3, and PC-GO 4 nanocomposites.
Thin film code
PC (wt%)
GO (wt%)
Transparency (%)
Thickness (µm)
PC
20
0
92
17
PC-GO 1
20
0.1
89
19
PC-GO 2
20
0.3
88
25
PC-GO 3
20
0.5
85
25
PC-GO 4
20
0.7
81
28Figure 2
Photographs of PC (a) and PC-GO 1, PC-GO 2, PC-GO 3, and PC-GO 4 (b–e) nanocomposite thin films.
(a)
(b)
(c)
(d)
(e)


### 3.2. Thermal Studies

Thermogravimetric analysis of the PC, GO, and PC-GO composite is represented in Figure3. In the GO, the weight loss around 100°C was caused by the elimination of water molecules and gradual reduction from 130°C to 210°C. Further, the weight loss of the GO extended to 600°C. The major mass reduction was observed at 210°C due to the decomposition of the oxygen-containing functional groups [31]. The PC was thermally stable up to 280°C and its maximum degradation temperature was observed at 380°C (decomposition of PC backbone) [32]. The PC-GO composite was degraded by two stages: the initial weight loss started at 210°C due to the loss of oxygen functionalities of the GO. In the second stage, the weight loss started at 380°C, due to the decomposition temperature of the PC backbone. The weight loss around 210°C concludes the confinement of the GO in the polymer matrix. Hence, the reinforcement of GO in the PC matrix was clearly validated by thermogravimetric analysis.Figure 3
Thermogravimetric analysis of PC, GO, and PC-GO 3 composite.


### 3.3. Fourier Transform-Infrared (FT-IR) and Raman Spectroscopy

FT-IR spectra of the PC, GO, and PC-GO composite are shown in Figure4. In the GO, the peaks appeared at 1720, 1630, 3400, and 1220 cm−1 attributed to C=O, C=C, O-H, and C-O (epoxy), respectively [33]. In pristine PC, the strong absorption bands due to the C=O stretching vibration of the carbonate group were observed at 1,780 cm−1 for diphenyl dicarbonates [34]. The absorption bands of the C-O-C stretching vibrations were also identified at the range 1150–1270 cm−1. Further, C=O and C-H stretching of methyl groups peaks were observed at ~1770 cm−1 and ~3100 cm−1, respectively.Figure 4
FT-IR spectra of GO, PC, and PC-GO 3 composite.Figure5 shows the Raman spectra of the GO, PC, and PC-GO composite. In the PC spectra, C-H stretching vibration appears in the range of 3000–2843 cm−1, C-O symmetrical stretching at 1608 cm−1, C-O-C vibration at 1248 cm−1, and C-H bending vibration at 830 cm−1. In the GO spectra, G band was observed at ~1590 cm−1 and D band appeared at ~1350 cm−1. In the PC-GO spectra, slight shifting of the characteristics of G and D bands was observed at ~1600 cm−1 and ~1390 cm−1, respectively. Further, the intensity decreased due to low concentration of the GO in the polymer matrix [35, 36].Figure 5
Raman spectra of GO, PC, and PC-GO 3 composite.


### 3.4. Morphological Analysis

FE-SEM was carried out to explore the agglomeration and dispersion of GO in the PC matrix. Figures6(a) and 6(b) show the as-prepared GO nanoflakes at low and high magnifications. The micrographs also indicate that the GO has ultrathin lamellar structure at micro- and nanolevels, and the GO has well defined porous network and interlinked three-dimensional structure [37].Figure 6
SEM micrographs of as-prepared graphene oxides at different magnifications (a and b).
(a)
(b)Figures7(a)–7(e) depict the SEM images of the surface of pristine PC and PC-GO 1, PC-GO 2, PC-GO 3, and PC-GO 4 thin films. The thicknesses of the PC and PC-GO 4 thin films were 17 μm and 28 μm, respectively. The surface roughness of the membranes slightly increased with respect to the GO content (0 to 0.7 wt%), which was clearly identified from Figures 7(a)–7(e). Notably, the agglomeration of GO in the PC matrix can be clearly observed from the large and irregular grains on the surface of the thin films by increasing the concentration of the GO. These grains may have formed because of the aggregation of GO molecules on the surface of the PC films, which might be due to the weak intermolecular hydrogen bonding between the oxygen-containing functional groups (-OH and -COOH) of GO and the carbonyl (-C=O) group of PC.Figure 7
SEM micrographs: surface morphology of PC (a) and PC-GO 1, PC-GO 2, PC-GO 3, and PC-GO 4 (b–e) thin films.
(a)
(b)
(c)
(d)
(e)The cross-sectional morphologies of the PC and PC-GO 1, PC-GO 2, PC-GO 3, and PC-GO 4 thin films are shown in Figures8(a)–8(e). It was observed that the cross section of the PC matrix exhibits negligible roughness compared with the PC-GO 1, PC-GO 2, PC-GO 3, and PC-GO 4 nanocomposites. Furthermore, the PC-GO 1, PC-GO 2, PC-GO 3, and PC-GO 4 thin films show a rough fracture with irregular protuberances, owing to the aggregation of GO sheets in the PC matrix. The layered structure of the GO was lucidly identified at the cross section of the PC-GO 1, PC-GO 2, PC-GO 3, and PC-GO 4 thin films. In addition, the agglomeration of GO in the polymer matrix gradually increased along with the increasing weight ratio of GO in the PC matrix. Notably, the GO nanoflakes in the PC matrix were observed as crumpled and folded due to higher content of the GO.Figure 8
SEM micrographs: cross-sectional images of PC (a) and PC-GO 1, PC-GO 2, PC-GO 3, and PC-GO 4 (b–e) thin films.
(a)
(b)
(c)
(d)
(e)


### 3.5. Antibacterial Activities

Figures9(a)–9(f) show the antibacterial activities of the GO, PC, and PC-GO samples against Gram-positiveS. aureus (Figures 9(a), 9(c), and 9(e)) and Gram-negativeE. coli (Figures 9(b), 9(d), and 9(f)). It was observed that the GO and PC-GO samples possessed growth inhibition zones forS. aureusandE. coli, owing to the antibacterial nature of GO. The inhibition zone was clearly identified at the edges of the samples, where the bacteria have not grown enough to be visible. A possible mechanism could be the following: the GO came into contact with the bacteria; it initiates the oxidation of glutathione (prominent cellular antioxidant) and the GO also acts as conducting bridge for extracting electrons from the glutathione and delivering them to the environment [4, 11, 38]. Notably, the pristine PC sample did not show any inhibition zones, due to the absence of GO. The antibacterial activities of the PC, GO, and PC-GO 3 samples againstS. aureus andE. coliare shown in Figure 10. ForS. aureus, the PC, GO, and PC-GO 3 exhibited inhibition zones of ~0, ~3.4, and ~2.2 mm, respectively. ForE. coli, the inhibition zones were observed for the PC, GO, and PC-GO 3 around ~0, ~3.3, and ~4.8 mm, respectively.Figure 9
Antibacterial activity of GO (a, b), PC-GO 3 (c, d), and PC (e, f) againstS. aureus andE. coli.Figure 10
Antibacterial activities of PC, GO, and PC-GO 3 samples againstE. coli andS. aureus.


## 3.1. Transparency of PC and PC-GO Thin Films

Figure2 shows the images of the PC and PC-GO 1 (0.1 wt%), PC-GO 2 (0.3 wt%), PC-GO 3 (0.5 wt%), and PC-GO 4 (0.7 wt%) (for convenience, nanocomposites thin film compositions are denoted by PC-GO 1, PC-GO 2, PC-GO 3, and PC-GO 4, resp.) nanocomposite transparent films, which changed in color from lighter to darker (black color) with respect to the loading of GO in the PC matrix; at higher loading of GO (0.7 wt%), the darkest and more agglomerated film was obtained (Figure 2(e)). This might be due to the visible region absorption of C=C bonds in the GO, owing to the average number of C=C double bonds being increased by increasing the concentration of GO [30]. Table 1 shows the composition of PC and PC-GO 1, PC-GO 2, PC-GO 3, and PC-GO 4 nanocomposites and thickness of the as-prepared thin films. The thicknesses of the PC and PC-GO thin films were clearly measured by using Digimatic Micrometer (Mitutoyo, Series 293 MDC-MX Lite) and the FE-SEM images. The thickness of the PC-GO 1, PC-GO 2, PC-GO 3, and PC-GO 4 thin films minutely increased with respect to the content of GO in the polymer matrix.Table 1
Composition of PC and PC-GO 1, PC-GO 2, PC-GO 3, and PC-GO 4 nanocomposites.
Thin film code
PC (wt%)
GO (wt%)
Transparency (%)
Thickness (µm)
PC
20
0
92
17
PC-GO 1
20
0.1
89
19
PC-GO 2
20
0.3
88
25
PC-GO 3
20
0.5
85
25
PC-GO 4
20
0.7
81
28Figure 2
Photographs of PC (a) and PC-GO 1, PC-GO 2, PC-GO 3, and PC-GO 4 (b–e) nanocomposite thin films.
(a)
(b)
(c)
(d)
(e)


## 3.2. Thermal Studies

Thermogravimetric analysis of the PC, GO, and PC-GO composite is represented in Figure3. In the GO, the weight loss around 100°C was caused by the elimination of water molecules and gradual reduction from 130°C to 210°C. Further, the weight loss of the GO extended to 600°C. The major mass reduction was observed at 210°C due to the decomposition of the oxygen-containing functional groups [31]. The PC was thermally stable up to 280°C and its maximum degradation temperature was observed at 380°C (decomposition of PC backbone) [32]. The PC-GO composite was degraded by two stages: the initial weight loss started at 210°C due to the loss of oxygen functionalities of the GO. In the second stage, the weight loss started at 380°C, due to the decomposition temperature of the PC backbone. The weight loss around 210°C concludes the confinement of the GO in the polymer matrix. Hence, the reinforcement of GO in the PC matrix was clearly validated by thermogravimetric analysis.Figure 3
Thermogravimetric analysis of PC, GO, and PC-GO 3 composite.


## 3.3. Fourier Transform-Infrared (FT-IR) and Raman Spectroscopy

FT-IR spectra of the PC, GO, and PC-GO composite are shown in Figure4. In the GO, the peaks appeared at 1720, 1630, 3400, and 1220 cm−1 attributed to C=O, C=C, O-H, and C-O (epoxy), respectively [33]. In pristine PC, the strong absorption bands due to the C=O stretching vibration of the carbonate group were observed at 1,780 cm−1 for diphenyl dicarbonates [34]. The absorption bands of the C-O-C stretching vibrations were also identified at the range 1150–1270 cm−1. Further, C=O and C-H stretching of methyl groups peaks were observed at ~1770 cm−1 and ~3100 cm−1, respectively.Figure 4
FT-IR spectra of GO, PC, and PC-GO 3 composite.Figure5 shows the Raman spectra of the GO, PC, and PC-GO composite. In the PC spectra, C-H stretching vibration appears in the range of 3000–2843 cm−1, C-O symmetrical stretching at 1608 cm−1, C-O-C vibration at 1248 cm−1, and C-H bending vibration at 830 cm−1. In the GO spectra, G band was observed at ~1590 cm−1 and D band appeared at ~1350 cm−1. In the PC-GO spectra, slight shifting of the characteristics of G and D bands was observed at ~1600 cm−1 and ~1390 cm−1, respectively. Further, the intensity decreased due to low concentration of the GO in the polymer matrix [35, 36].Figure 5
Raman spectra of GO, PC, and PC-GO 3 composite.


## 3.4. Morphological Analysis

FE-SEM was carried out to explore the agglomeration and dispersion of GO in the PC matrix. Figures6(a) and 6(b) show the as-prepared GO nanoflakes at low and high magnifications. The micrographs also indicate that the GO has ultrathin lamellar structure at micro- and nanolevels, and the GO has well defined porous network and interlinked three-dimensional structure [37].Figure 6
SEM micrographs of as-prepared graphene oxides at different magnifications (a and b).
(a)
(b)Figures7(a)–7(e) depict the SEM images of the surface of pristine PC and PC-GO 1, PC-GO 2, PC-GO 3, and PC-GO 4 thin films. The thicknesses of the PC and PC-GO 4 thin films were 17 μm and 28 μm, respectively. The surface roughness of the membranes slightly increased with respect to the GO content (0 to 0.7 wt%), which was clearly identified from Figures 7(a)–7(e). Notably, the agglomeration of GO in the PC matrix can be clearly observed from the large and irregular grains on the surface of the thin films by increasing the concentration of the GO. These grains may have formed because of the aggregation of GO molecules on the surface of the PC films, which might be due to the weak intermolecular hydrogen bonding between the oxygen-containing functional groups (-OH and -COOH) of GO and the carbonyl (-C=O) group of PC.Figure 7
SEM micrographs: surface morphology of PC (a) and PC-GO 1, PC-GO 2, PC-GO 3, and PC-GO 4 (b–e) thin films.
(a)
(b)
(c)
(d)
(e)The cross-sectional morphologies of the PC and PC-GO 1, PC-GO 2, PC-GO 3, and PC-GO 4 thin films are shown in Figures8(a)–8(e). It was observed that the cross section of the PC matrix exhibits negligible roughness compared with the PC-GO 1, PC-GO 2, PC-GO 3, and PC-GO 4 nanocomposites. Furthermore, the PC-GO 1, PC-GO 2, PC-GO 3, and PC-GO 4 thin films show a rough fracture with irregular protuberances, owing to the aggregation of GO sheets in the PC matrix. The layered structure of the GO was lucidly identified at the cross section of the PC-GO 1, PC-GO 2, PC-GO 3, and PC-GO 4 thin films. In addition, the agglomeration of GO in the polymer matrix gradually increased along with the increasing weight ratio of GO in the PC matrix. Notably, the GO nanoflakes in the PC matrix were observed as crumpled and folded due to higher content of the GO.Figure 8
SEM micrographs: cross-sectional images of PC (a) and PC-GO 1, PC-GO 2, PC-GO 3, and PC-GO 4 (b–e) thin films.
(a)
(b)
(c)
(d)
(e)


## 3.5. Antibacterial Activities

Figures9(a)–9(f) show the antibacterial activities of the GO, PC, and PC-GO samples against Gram-positiveS. aureus (Figures 9(a), 9(c), and 9(e)) and Gram-negativeE. coli (Figures 9(b), 9(d), and 9(f)). It was observed that the GO and PC-GO samples possessed growth inhibition zones forS. aureusandE. coli, owing to the antibacterial nature of GO. The inhibition zone was clearly identified at the edges of the samples, where the bacteria have not grown enough to be visible. A possible mechanism could be the following: the GO came into contact with the bacteria; it initiates the oxidation of glutathione (prominent cellular antioxidant) and the GO also acts as conducting bridge for extracting electrons from the glutathione and delivering them to the environment [4, 11, 38]. Notably, the pristine PC sample did not show any inhibition zones, due to the absence of GO. The antibacterial activities of the PC, GO, and PC-GO 3 samples againstS. aureus andE. coliare shown in Figure 10. ForS. aureus, the PC, GO, and PC-GO 3 exhibited inhibition zones of ~0, ~3.4, and ~2.2 mm, respectively. ForE. coli, the inhibition zones were observed for the PC, GO, and PC-GO 3 around ~0, ~3.3, and ~4.8 mm, respectively.Figure 9
Antibacterial activity of GO (a, b), PC-GO 3 (c, d), and PC (e, f) againstS. aureus andE. coli.Figure 10
Antibacterial activities of PC, GO, and PC-GO 3 samples againstE. coli andS. aureus.


## 4. Conclusion

In this work, graphene oxide reinforced polycarbonate based antibacterial and optically transparent thin films were fabricated by a facile technique. Thermal analysis and SEM micrographs confirmed the reinforcement of GO in the PC matrix. The agglomeration of GOs increased by increasing their concentration and the threshold limit of GO content in the PC matrix was 0.5%. The GO and PC-GO films exhibited excellent bacteriostatic effect againstE. coli andS. aureus. The antibacterial nature of the PC-GO films makes these materials promising candidates for use in biomedical devices and food packaging materials.



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