# Green Production of Indolylquinones, Derivatives of Perezone, and Related Molecules, Promising Antineoplastic Compounds

Authors: René Gerardo Escobedo-González; Héctor Pérez Martínez; Ma. Inés Nicolás-Vázquez; Joel Martínez; Gabriela Gómez; Juan Nava Serrano; Vladimir Carranza Téllez; C. L. Vargas-Requena; René Miranda Ruvalcaba
Journal: Journal of Chemistry
 (2016)

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## Abstract

A green approach to produce the indolyl derivatives from four natural quinones (perezone, isoperezone, menadione, and plumbagin) was performed; in this regard, a comparative study was accomplished among the typical mantle heating and three nonconventional activating modes of reaction (microwave, near-infrared, and high speed ball milling or tribochemical), under solventless conditions and using bentonitic clay as a catalyst. In addition, the tribochemical production of isoperezone from perezone is also commented on. It is also worth noting that the cytotoxicity of the synthesized indolylquinones in human breast cancer cell was tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, with the 3-indolylisoperezone being the most active. The structural attribution of the target molecules was performed by typical spectroscopic procedures; moreover, the experimental and computed1H and 13C NMR chemical shifts data, with previous acquisition of the corresponding minimum energetic structures, were in good agreement.


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## Body

## 1. Introduction

Green Chemistry, with a protocol of a cohesive set of twelve principles [1], is an actual discipline that encourages working at the molecular level, in order to achieve sustainability; since the early 1990s, this paradigm has acquired its up-to-date position as a scientific discipline, preventing pollution via the implementation of reactions without damaging conditions to the environment [2]. Under such a protocol, an ideal synthesis would be that by which a target molecule is produced quantitatively in one step from available and inexpensive starting compounds in an ecologically sustainable process; hence, the request of novel sustainable processes is among the major challenges in organic synthesis. In this regard, researchers at the vanguard of innovation must have the knowledge to design chemicals processes with little or no risk to the environment. However, no activity can be completely risk-free or waste-free or have lower harmful emissions. Consequently, the “Twelve Principles of Green Chemistry” should be viewed and applied as a scientific reflection [3].Many quinones are secondary metabolites generally isolated from plants [4]. In particular, perezone or pipitzaoic acid, a sesquiterpene quinone, is recognized as the first secondary metabolite isolated in the New World by Río de la Loza [5], from the roots ofPerezia (currentlyAcourtia) specimens; this important molecule has been the target of many chemical, structural, and biological studies, for example, its transformation into pipitzols [6–8] and isoperezone [9], its structural elucidation [10, 11], several green contributions [12], its use as a pigment [13], and highlighting several pharmacological effects [14–21]. By the way, plumbagin, a naphthoquinone from the genusPlumbago [22, 23], has been ascribed to several biological properties, for example, its antineoplasic activity [24].In recent years, cancer prevention by means of natural products has received considerable attention; consequently, the chemistry of secondary metabolites is a powerful source for novel drug candidates. It is also convenient to underline the notion that the design and implementation of synthetic processes moving toward the Green Chemistry Protocol [2, 25] are one of the major challenges in modern organic synthesis.The indolylquinone moiety is present in many natural products with interesting biological activities [26, 27]; consequently, the promising pharmaceutical use of the indolylquinones has attracted attention for the synthesis of new molecules of this class, mainly under a green approach. In this sense, the most common mode for the preparation of 3-indolylquinones is the direct coupling of indole with several quinones, in the presence of a catalyst (HCl, H2SO4, AcOH, Bi(OTf)3, I2, or InBr3) in the presence of solvent, using the mantle heating procedure in order to activate the reactions [28].As part of our ongoing research program, we are interested in the implementation of green synthetic strategies, in order to synergize or modify the pharmacological activities of single known compounds throughout the construction of novel hybrid molecules, mainly using nonconventional activating sources such as microwave (MW) and near-infrared (NIR) irradiation, ultrasound (US), and high speed ball milling (HSBM) in the absence of solvent or using innocuous solvents [29–34] and when required employing Tonsil Actisil FF (TAFF), commercial bentonitic clay, as a green catalyst [35].Taking into account the aforementioned commentaries, the goals of this work are, in a first stage, to offer a green contribution for the production of four pharmacologic candidates, the indolyl derivatives of perezone (1), isoperezone (2), menadione (3), and plumbagin (4), by comparison of typical mantle heating conditions (MH) with uncommon activating modes, MW and NIR irradiation, in addition to the mechanical procedure of HSBM, all them in the absence of solvent and using TAFF as a catalyst. Additionally, we make an evaluation of the cytotoxic effect of the title molecules, in breast cancer cells as complement to theoretical-computation characterization of them. And finally, novel and convenient transformation of perezone into isoperezone, using for the first time HSBM, is also recommended.


## 2. Materials and Methods

General. The reagents indole and menadione were purchased from Sigma-Aldrich Chemical Co. and were used without further preparation. The secondary metabolites, perezone and plumbagin, were isolated from the vegetal specimensAcourtia adnata andPlumbago pulchella Boiss., respectively, according to literature procedures [36–39]. The reactions were monitored by thin layer chromatography (TLC) inn-hexane/ethyl acetate (80 : 20) using silica gel 60-F254 coated aluminum sheets; the corresponding visualization was achieved using a 254 nm UV lamp. The catalyst, Actisil FF (TAFF), bentonitic clay, was purchased from Tonsil Mexicana S.A. de C.V., Km 7 High Road, Puebla-Tlaxcala, Puebla, Mexico.1H and 13C NMR spectra were performed using a Varian Mercury-300 spectrometer at 300 MHz and 75 MHz for hydrogen and carbon, respectively, employing CDCl3 as a solvent and TMS as an internal reference. The multiplicities are reported as singlet (s), broad singlet (bs), doublet (d), triplet (t), and multiplet (m). The EIMS and HRMS data were determined using JEOL JMS-700 MStation mass spectrometer. The elemental composition was calculated within a mass range of ±10 ppm from the accurate measured mass. The melting points were determined in a Fisher-Johns apparatus and are uncorrected. The microwave-assisted production of the target compounds was performed using CEM Focused Microwave™ Synthesis System. The near-infrared irradiation was generated using a commercial device “Flavor-Wave®” (1300 W/110 V/120 V-60 Hz|220 V/240 V-60 Hz) [45]. The high speed ball milling was generated using Ball Mill PM 100 Retch, with 6 carbon steel balls (weight: 23.85 g, 0.39′′ diameter). The temperature was determined, for NIR, employing infrared thermometer (Infrared + Type K Thermometer, Extech Instruments, Sigma-Aldrich 2509388-1 EA); herein, the laser pointer was directed to the reaction center.The human MDA-MB-231 breast cancer and dermal fibroblast normal cells lines were obtained from ATTC; NaH2PO4 and Na2HPO4 for phosphate buffer solution (PBS), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), and dimethyl sulfoxide (DMSO) were obtained from Sigma-Aldrich without further purification. The cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin G, and 100 μg/L streptomycin in humidified atmosphere with 5% CO2 at 37°C. The culture medium was replaced every two days and the cells were used at passages between two and five. Statistical analysis of IC50 was executed by PRISMA statistical program.The theoretical calculations were performed employing GAUSSIAN09 program [46].


### 2.1. Typical Experiments Using Thermal Conditions

A mixture of perezone1 (120 mg, 0.4839 mmol), isoperezone2 (120 mg, 0.4839 mmol), menadione3 (90 mg, 0.5233 mmol) or plumbagin4 (140 mg, 0.5000 mmol), indole5 (60 mg, 0.5128 mmol), and 50 mg of TAFF as catalyst was placed in a micro scale glass vessel. The mixtures were treated using mantle heating during a period of 60 min at 95°C. The reactions were monitored by TLC using silica gel/n-hexane/ethyl acetate (80 : 20). The corresponding pure molecules were obtained by column chromatography using the same mobile phase as TLC.


### 2.2. Typical Experiments Using Nonconventional Activating Sources

A mixture of perezone1 (120 mg, 0.4839 mmol), isoperezone2 (120 mg, 0.4839 mmol), menadione3 (90 mg, 0.5233 mmol) or plumbagin4 (140 mg, 0.5000 mmol), indole5 (60 mg, 0.5128 mmol), and 50 mg of TAFF as catalyst was placed in a micro scale glass vessel. The mixtures were treated using and comparing different activation modes: near-infrared irradiation during a period of 10 min at 121°C; microwave irradiation during a period of 10 min at 100°C and 250 W power, and high speed ball milling during a period of 60 min with 500 rpm and 27% power; all reactions were carried out in open vessel. The reactions were monitored by TLC using silica gel/n-hexane/ethyl acetate (80 : 20). The corresponding products were purified by column chromatography using the same mobile phase as TLC.2-Hydroxy-5-(1H-indol-3-yl)-6-methyl-3-(6-methylhept-5-en-2-yl)cyclohexa-2,5-diene-1,4-dione (
6
). Purple solid; mp 65–67°C. 1H NMR (CDCl3/TMS) (δ/ppm): 8.905 (bs, 1H, NH), 8.250 (s, 1H, OH), 7.430 (d, 1H, H-7′), 7.297 (d, 1H, H-4′), 7.225–7.162 (m, 2H, H-5′,6′), 7.162 (s, 1H, H-2′), 5.104 (bs, 1H, H-12), 3.100–3.220 (m, 1H, H-8), 2.181 (s, 3H, H-7), 1.853–1.770 (m, 4H, H-10,11), 1.645 (s, 3H, H-14), 1.548 (s, 3H, H-15), 1.263 (d, 3H, H-9). 13C NMR (CDCl3/TMS) (δ/ppm): 187.00 (C-1), 184.70 (C-4), 151.10 (C-3), 140.30 (C-5), 135.90 (C-6), 135.2 (C-9′), 131.60 (C-8′), 128.20 (C-2′), 126.70 (C-13), 124.90 (C-12), 124.20 (C-2), 122.70 (C-6′), 120.90 (C-5′), 120.80 (C-4′), 111.90 (C-7′), 108.90 (C-3′), 34.50 (C-10), 30.10 (C-8), 27.00 (C-11), 25.90 (C-14), 18.70 (C-9), 17.90 (C-15), 14.60 (C-7). EIMS (70 eV) m
/
z (%): 363 (69) M
+
•, 348 (6) [M-15]+, 334 (7) [M-29]+, 281 (100) M-82
+
•, 246 (6) M-
112
+
•. HRMS-EI m
/
z 363 M
+
•, calculated for C23H25NO3: 363.1834 Da; found for C23H25NO3 363.1816 Da (−5 ppm error).2-Hydroxy-5-(1H-indol-3-yl)-3-methyl-6-(6-methylhept-5-en-2-yl)cyclohexa-2,5-diene-1,4-dione (
7
). Purple solid; mp 62–65°C. 1H NMR (CDCl3/TMS) (δ/ppm): 8.476 (bs, 1H, NH), 8.100 (s, 1H, OH), 7.424 (d, 1H, H-7′), 7.350 (d, 1H, H-4′), 7.26–7.128 (m, 2H, H-5′,6′), 6.797 (s, 1H, H-2′), 4.830 (bs, 1H, H-12), 2.810–2.920 (m, 1H, H-8), 1.988 (s, 3H, H-7), 1.742–1.627 (m, 4H, H-10,11), 1.497 (s, 3H, H-14), 1.430 (s, 3H, H-15), 1.260 (d, 3H, H-9). 13C NMR (CDCl3/TMS) (δ/ppm): 188.00 (C-1), 183.70 (C-4), 151.40 (C-6), 149.60 (C-2), 135.70 (C-9′), 132.30 (C-13), 130.90 (C-8′), 128.20 (C-3), 123.80 (C-12), 122.80 (C-2′), 122.60 (C-6′), 120.90 (C-5′), 120.40 (C-4′), 116.50 (C-5), 114.10 (C-7′), 108.60 (C-3′), 38.70 (C-10), 30.36 (C-8), 29.00 (C-11), 23.98 (C-14), 23.01 (C-9), 14.09 (C-15), 11.12 (C-7). EIMS (70 eV) m
/
z (%): 363 (25) M
+
•, 348 (2) [M-15]+, 279 (28) M-
84
+
•, 261 (100) M-102
+
•, 149 (90) M-214
+
•, 167 (60) M-196
+
•. HRMS-IE: m
/
z 363 M
+
•, calculated for C23H25NO3: 363.1834 Da; found for C23H25NO3: 363.1829 Da (−1.5 ppm error).2-(1H-Indol-3-yl)-3-methylnaphthalene-1,4-dione (
8
). Red dark solid, mp 123°C. 1H NMR (CDCl3/TMS) (δ/ppm): 8.800 (bs, 1H, NH), 8.220–8.120 (m, 2H, H-5,8), 7.800–7.740 (m, 2H, H-6,7), 7.456 (d, 1H, H-7′), 7.430 (s, 1H, H-2′), 7.319 (d, 1H, H-4′), 7.220–7.104 (m, 2H, H-5′, 6′), 2.213 (s, 3H, H-9). 13C NMR (CDCl3/TMS) (δ/ppm): 186.00 (C-1), 184.80 (C-4), 143.30 (C-2), 140.40 (C-3), 135.80 (C-9′), 133.50 (C-7), 133.40 (C-6), 132.52 (C-10), 132.48 (C-11), 127.20 (C-8′), 126.70 (C-5), 126.20 (C-8), 122.30 (C-2′), 120.40 (C-6′), 120.30 (C-5′), 111.70 (C-4′), 111.60 (C-7′), 108.30 (C-3′), 15.80 (C-9). EIMS (70 eV) m
/
z (%): 287 (30) M
+
•, 270 (100) [M-17]+, 269 (12) M-
18
+
•, 258 (8) [M-29]+, 246 (40) [M-41]+. HRMS-EI: m
/
z 287 M
+
•, calculated for C19H13NO2: 287.0946 Da; found for C19H13NO2: 287.0974 Da (+9.8 ppm error).5-Hydroxy-3-(1H-indol-3-yl)-2-methylnaphthalene-1,4-dione (
9
). Dark solid, mp 134–137°C. 1H NMR (CDCl3/TMS) (δ/ppm): 12.250 (s, 1H, OH), 8.600 (bs, 1H, NH), 7.709 (d, 1H, H-8), 7.600–7.520 (m, 1H, H-7), 7.450 (d, 1H, H-7′), 7.414 (s, 1H, H-2′), 7.300 (d, 1H, H-4′), 7.260–7.190 (m, 2H, H-5′,6′), 7.200 (d, 1H, H-6), 2.196 (s, 3H, H-9). 13C NMR (CDCl3/TMS) (δ/ppm): 189.87 (C-4), 185.00 (C-1), 161.50 (C-5), 145.05 (C-2), 140.10 (C-3), 136.10 (C-9′), 135.70 (C-7), 132.50 (C-10), 126.90 (C-2′), 126.50 (C-8′), 123.90 (C-6), 122.70 (C-6′), 120.70 (C-5′), 120.30 (C-4′), 118.90 (C-8), 115.20 (C-11), 111.70 (C-7′), 107.90 (C-3′), 15.90 (C-9). EIMS (70 eV) m
/
z (%): 303 (100) M
+
•, 286 (44) [M-17]+, 274 (20) [M-29]+, 186 (8) M-117
+
•. HRMS-EI: m
/
z 303 M
+
•, calculated for C19H13NO2: 303.0895 Da; found for C19H13NO2: 303.0873 Da (−7.4 ppm error).


### 2.3. The 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl Tetrazolium Bromide Assay (MTT Assay)

This test was performed with MDA-MB231 breast cancer cell line according to Mosmann [47]. Cells were incubated at different indolylquinone concentrations. To derive viability curves of6–9 treated cells, the cells were seeded onto 96-well culture plate at density of 5000 cells/well and grown in DMEM supplemented with 10% FBS. Cells viability was measured using MTT reagent dissolved in PBS (0.5 mg/mL). On the day of measurement, the medium was carefully replaced by fresh DMEM + 10% FBS with diluted MTT (1 : 10, 10% MTT) and incubated for 1 h at 37°C in a CO2 incubator to allow the transformation of MTT dye to formazan salt. After removing incubation medium, formazan crystals were dissolved in 100 μL solution of DMSO. It is also important to highlight the notion that MTT reduction was quantified by measuring the light absorbance at 570 nm using the Benchmark Plus absorbance microplate reader (Bio-Rad). MTT test was repeated nine times. Percentage of proliferation (% viability) was calculated according to(1)
%
viability
=
B
A
×
100
,where A is the absorbance value of the control group and B is the absorbance value of the cells treated with indolyl-derivatives.


### 2.4. IC50 Determination

IC50 was extrapolated from the dose-response graph (Figure 1). The drug concentration that reduced the viability of cells by 50% (IC50) was determined by plotting triplicate data points over a concentration range and calculating values using regression analysis.Figure 1
Dose-response graphs at 24, 48, and 72 h of6,7,8, and9.


### 2.5. Computational Calculations

The reported calculations were carried out using Density Functional Theory (DFT) [48], employing Becke’s three-parameter exchange and Lee-Yang-Parr correlation hybrid functional (B3LYP) [49, 50] with the 6-311++G(d,p) basis set including the split-valance and diffuse functions [51–53]. The corresponding quantum-mechanical calculations to determine the 1H and 13C chemical shifts were performed by Gauge-Invariant-Atomic Orbital method (GIAO method) [54].


## 2.1. Typical Experiments Using Thermal Conditions

A mixture of perezone1 (120 mg, 0.4839 mmol), isoperezone2 (120 mg, 0.4839 mmol), menadione3 (90 mg, 0.5233 mmol) or plumbagin4 (140 mg, 0.5000 mmol), indole5 (60 mg, 0.5128 mmol), and 50 mg of TAFF as catalyst was placed in a micro scale glass vessel. The mixtures were treated using mantle heating during a period of 60 min at 95°C. The reactions were monitored by TLC using silica gel/n-hexane/ethyl acetate (80 : 20). The corresponding pure molecules were obtained by column chromatography using the same mobile phase as TLC.


## 2.2. Typical Experiments Using Nonconventional Activating Sources

A mixture of perezone1 (120 mg, 0.4839 mmol), isoperezone2 (120 mg, 0.4839 mmol), menadione3 (90 mg, 0.5233 mmol) or plumbagin4 (140 mg, 0.5000 mmol), indole5 (60 mg, 0.5128 mmol), and 50 mg of TAFF as catalyst was placed in a micro scale glass vessel. The mixtures were treated using and comparing different activation modes: near-infrared irradiation during a period of 10 min at 121°C; microwave irradiation during a period of 10 min at 100°C and 250 W power, and high speed ball milling during a period of 60 min with 500 rpm and 27% power; all reactions were carried out in open vessel. The reactions were monitored by TLC using silica gel/n-hexane/ethyl acetate (80 : 20). The corresponding products were purified by column chromatography using the same mobile phase as TLC.2-Hydroxy-5-(1H-indol-3-yl)-6-methyl-3-(6-methylhept-5-en-2-yl)cyclohexa-2,5-diene-1,4-dione (
6
). Purple solid; mp 65–67°C. 1H NMR (CDCl3/TMS) (δ/ppm): 8.905 (bs, 1H, NH), 8.250 (s, 1H, OH), 7.430 (d, 1H, H-7′), 7.297 (d, 1H, H-4′), 7.225–7.162 (m, 2H, H-5′,6′), 7.162 (s, 1H, H-2′), 5.104 (bs, 1H, H-12), 3.100–3.220 (m, 1H, H-8), 2.181 (s, 3H, H-7), 1.853–1.770 (m, 4H, H-10,11), 1.645 (s, 3H, H-14), 1.548 (s, 3H, H-15), 1.263 (d, 3H, H-9). 13C NMR (CDCl3/TMS) (δ/ppm): 187.00 (C-1), 184.70 (C-4), 151.10 (C-3), 140.30 (C-5), 135.90 (C-6), 135.2 (C-9′), 131.60 (C-8′), 128.20 (C-2′), 126.70 (C-13), 124.90 (C-12), 124.20 (C-2), 122.70 (C-6′), 120.90 (C-5′), 120.80 (C-4′), 111.90 (C-7′), 108.90 (C-3′), 34.50 (C-10), 30.10 (C-8), 27.00 (C-11), 25.90 (C-14), 18.70 (C-9), 17.90 (C-15), 14.60 (C-7). EIMS (70 eV) m
/
z (%): 363 (69) M
+
•, 348 (6) [M-15]+, 334 (7) [M-29]+, 281 (100) M-82
+
•, 246 (6) M-
112
+
•. HRMS-EI m
/
z 363 M
+
•, calculated for C23H25NO3: 363.1834 Da; found for C23H25NO3 363.1816 Da (−5 ppm error).2-Hydroxy-5-(1H-indol-3-yl)-3-methyl-6-(6-methylhept-5-en-2-yl)cyclohexa-2,5-diene-1,4-dione (
7
). Purple solid; mp 62–65°C. 1H NMR (CDCl3/TMS) (δ/ppm): 8.476 (bs, 1H, NH), 8.100 (s, 1H, OH), 7.424 (d, 1H, H-7′), 7.350 (d, 1H, H-4′), 7.26–7.128 (m, 2H, H-5′,6′), 6.797 (s, 1H, H-2′), 4.830 (bs, 1H, H-12), 2.810–2.920 (m, 1H, H-8), 1.988 (s, 3H, H-7), 1.742–1.627 (m, 4H, H-10,11), 1.497 (s, 3H, H-14), 1.430 (s, 3H, H-15), 1.260 (d, 3H, H-9). 13C NMR (CDCl3/TMS) (δ/ppm): 188.00 (C-1), 183.70 (C-4), 151.40 (C-6), 149.60 (C-2), 135.70 (C-9′), 132.30 (C-13), 130.90 (C-8′), 128.20 (C-3), 123.80 (C-12), 122.80 (C-2′), 122.60 (C-6′), 120.90 (C-5′), 120.40 (C-4′), 116.50 (C-5), 114.10 (C-7′), 108.60 (C-3′), 38.70 (C-10), 30.36 (C-8), 29.00 (C-11), 23.98 (C-14), 23.01 (C-9), 14.09 (C-15), 11.12 (C-7). EIMS (70 eV) m
/
z (%): 363 (25) M
+
•, 348 (2) [M-15]+, 279 (28) M-
84
+
•, 261 (100) M-102
+
•, 149 (90) M-214
+
•, 167 (60) M-196
+
•. HRMS-IE: m
/
z 363 M
+
•, calculated for C23H25NO3: 363.1834 Da; found for C23H25NO3: 363.1829 Da (−1.5 ppm error).2-(1H-Indol-3-yl)-3-methylnaphthalene-1,4-dione (
8
). Red dark solid, mp 123°C. 1H NMR (CDCl3/TMS) (δ/ppm): 8.800 (bs, 1H, NH), 8.220–8.120 (m, 2H, H-5,8), 7.800–7.740 (m, 2H, H-6,7), 7.456 (d, 1H, H-7′), 7.430 (s, 1H, H-2′), 7.319 (d, 1H, H-4′), 7.220–7.104 (m, 2H, H-5′, 6′), 2.213 (s, 3H, H-9). 13C NMR (CDCl3/TMS) (δ/ppm): 186.00 (C-1), 184.80 (C-4), 143.30 (C-2), 140.40 (C-3), 135.80 (C-9′), 133.50 (C-7), 133.40 (C-6), 132.52 (C-10), 132.48 (C-11), 127.20 (C-8′), 126.70 (C-5), 126.20 (C-8), 122.30 (C-2′), 120.40 (C-6′), 120.30 (C-5′), 111.70 (C-4′), 111.60 (C-7′), 108.30 (C-3′), 15.80 (C-9). EIMS (70 eV) m
/
z (%): 287 (30) M
+
•, 270 (100) [M-17]+, 269 (12) M-
18
+
•, 258 (8) [M-29]+, 246 (40) [M-41]+. HRMS-EI: m
/
z 287 M
+
•, calculated for C19H13NO2: 287.0946 Da; found for C19H13NO2: 287.0974 Da (+9.8 ppm error).5-Hydroxy-3-(1H-indol-3-yl)-2-methylnaphthalene-1,4-dione (
9
). Dark solid, mp 134–137°C. 1H NMR (CDCl3/TMS) (δ/ppm): 12.250 (s, 1H, OH), 8.600 (bs, 1H, NH), 7.709 (d, 1H, H-8), 7.600–7.520 (m, 1H, H-7), 7.450 (d, 1H, H-7′), 7.414 (s, 1H, H-2′), 7.300 (d, 1H, H-4′), 7.260–7.190 (m, 2H, H-5′,6′), 7.200 (d, 1H, H-6), 2.196 (s, 3H, H-9). 13C NMR (CDCl3/TMS) (δ/ppm): 189.87 (C-4), 185.00 (C-1), 161.50 (C-5), 145.05 (C-2), 140.10 (C-3), 136.10 (C-9′), 135.70 (C-7), 132.50 (C-10), 126.90 (C-2′), 126.50 (C-8′), 123.90 (C-6), 122.70 (C-6′), 120.70 (C-5′), 120.30 (C-4′), 118.90 (C-8), 115.20 (C-11), 111.70 (C-7′), 107.90 (C-3′), 15.90 (C-9). EIMS (70 eV) m
/
z (%): 303 (100) M
+
•, 286 (44) [M-17]+, 274 (20) [M-29]+, 186 (8) M-117
+
•. HRMS-EI: m
/
z 303 M
+
•, calculated for C19H13NO2: 303.0895 Da; found for C19H13NO2: 303.0873 Da (−7.4 ppm error).


## 2.3. The 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl Tetrazolium Bromide Assay (MTT Assay)

This test was performed with MDA-MB231 breast cancer cell line according to Mosmann [47]. Cells were incubated at different indolylquinone concentrations. To derive viability curves of6–9 treated cells, the cells were seeded onto 96-well culture plate at density of 5000 cells/well and grown in DMEM supplemented with 10% FBS. Cells viability was measured using MTT reagent dissolved in PBS (0.5 mg/mL). On the day of measurement, the medium was carefully replaced by fresh DMEM + 10% FBS with diluted MTT (1 : 10, 10% MTT) and incubated for 1 h at 37°C in a CO2 incubator to allow the transformation of MTT dye to formazan salt. After removing incubation medium, formazan crystals were dissolved in 100 μL solution of DMSO. It is also important to highlight the notion that MTT reduction was quantified by measuring the light absorbance at 570 nm using the Benchmark Plus absorbance microplate reader (Bio-Rad). MTT test was repeated nine times. Percentage of proliferation (% viability) was calculated according to(1)
%
viability
=
B
A
×
100
,where A is the absorbance value of the control group and B is the absorbance value of the cells treated with indolyl-derivatives.


## 2.4. IC50 Determination

IC50 was extrapolated from the dose-response graph (Figure 1). The drug concentration that reduced the viability of cells by 50% (IC50) was determined by plotting triplicate data points over a concentration range and calculating values using regression analysis.Figure 1
Dose-response graphs at 24, 48, and 72 h of6,7,8, and9.


## 2.5. Computational Calculations

The reported calculations were carried out using Density Functional Theory (DFT) [48], employing Becke’s three-parameter exchange and Lee-Yang-Parr correlation hybrid functional (B3LYP) [49, 50] with the 6-311++G(d,p) basis set including the split-valance and diffuse functions [51–53]. The corresponding quantum-mechanical calculations to determine the 1H and 13C chemical shifts were performed by Gauge-Invariant-Atomic Orbital method (GIAO method) [54].


## 3. Results and Discussion

### 3.1. Synthesis

The reactions of1–4 with indole (5), under solventless conditions and with TAFF as a catalyst, were performed using the following three nonconventional modes of activation: microwave and near-infrared irradiation and high speed ball milling, affording the respective indole derivatives6–9 (Scheme 1); in addition, the target molecules were comparatively obtained using mantle heating conditions; the corresponding results are summarized in Table 1.Table 1
Production of indolylquinones derivatives, using different activating modes.
Product
Yields %
MH
MW
NIR
HSBM
6
24
79
62
24
7
12
35
31
18
8
46
42
51
30
9
25
41
64
35
Best reaction conditions: MH, 95°C and 60 min; MW, 250 W, 100°C, and 10 min; NIR, 121°C and 10 min; HSBM, 500 rpm and 60 min.Scheme 1
Synthesis of indole-quinone derivatives.In general, the pathway must occur by classical Michael addition by the indol to the corresponding quinonic substrates, assisted by an interaction of the oxygen of a carbonylic group with the Lewis acidic sites of TAFF, followed by in situ oxidation, producing6–9 in moderated yields at low times. It is appropriate to note that6,7, and9 are novel molecules, and according to our knowledge8 has been previously produced by MH and US in the presence of solvent with the employment of a noninnocuous catalyst [28, 55, 56]. Moreover, literature research shows that this seems to be the first report for the green production of indolylquinones using MW, NIR, and HSBM as the activating modes without a solvent and in the presence of a natural ecofriendly catalyst.A complementary and interesting result was the transformation of perezone into isoperezone, using for the first time HSBM (Scheme2), in comparative yields and reaction times, with values previously reported [9, 12].Scheme 2
Transformation of perezone into isoperezone.For the structural attribution of2 and6–9, in a first instance, isoperezone was spectroscopically correlated with literature data [9] as well as its melting point (108-109°C). Considering the notion that6,7, and9 are novel products and that they are structurally related to8, appropriate spectroscopic characterizations of the target molecules were performed. Thus, in a first place, the corresponding accurate molecular ions were acquired by HRMS-EI to achieve the elemental compositions; this was obtained in agreement with the expected products.In regard to the1H NMR, it is worth noting the disappearance of the corresponding signals assigned to H-3 of indole and reagents2–4, in addition to the signal assigned to H-6 of perezone; moreover, the signal due to the proton linked to the nitrogen of the indole, δ 7.81 ppm, was displaced, for6–9 to lower fields 8.4–8.9 ppm, unequivocally due to deprotection generated by the high degree of conjugation between the indolic-quinonic moieties of the products. In this sense, the obtained 13C NMR data complemented the structural characterization of molecules6–9, and the signals of C-3 and C-2 of indole at 102.22 ppm and 111.10 ppm were, respectively, displaced to lower fields, 107.9–108.9 and 122.3–128.2 ppm, respectively. This change in the chemical shift was also attributed to deprotection by the quinonic system. In general, the 1H and 13C NMR experimental data correlated appropriately with those values predicted by computational models.


### 3.2. Cytotoxic Activity

Indolylquinones cytotoxicities were evaluated in human MDA-MB-231 breast cancer cells using the MTT assay, employing as control normal fibroblast cells. The studied compounds were assayed in concentrations of 0 to 100μg/mL for6, 0 to 150 μg/mL for7, 0 to 110 for8, and finally 0 to 130 μg/mL for9. According to Figure 1, the decrease of viable cells starts at 24 hours of incubation of the studied compounds; moreover molecule7 began to decrease at a lower concenttration (15 mg/mL); on the other hand, for the rest of the molecules, the decrease of the viability began at concentrations higher than 20 μg/mL. The same behavior was exhibited at 48 h with cancer cell viability in the range from 10 to 20% when the highest concentrations of 100, 150, 110, and 130 μg/mL to6,7,8, and9, respectively, were used. The results after 72 h showed in7 and9 similar trends to those at 48 h; however,6 and8 increased the cellular viability in the highest concentrations.The IC50 values were obtained through extrapolation from the dose-response graphs (Figure 1). The results of the viability in human breast cancer cells against the four molecules tested recognize7 as the most active molecule with IC50 of 25 μg/mL. In addition, for6,8, and9, the values of IC50 were 40.6, 41.6, and 32.3 μg/mL, respectively. These concentrations were tested against healthy dermal fibroblast. With this result, it is important to point out that only9 developed a cytotoxic effect with 15% mortality; on the other hand,6–8, instead of promoting cells death, caused fibroblast proliferation. The effect in healthy cells is very interesting and is currently under study by our research group, as the cytotoxic pathway presented for these compounds.


### 3.3. Computational Study

The molecules obtained were also studied through computational chemistry methods. In the optimization of the target molecules, a conformer distribution and the selection of the most stable conformer were achieved. Additionally, the selected geometries were appropriately optimized by DFT employing B3LYP functional and the 6-311++G(d,p) basis set. The structures obtained in phase gas are depicted in Figure2.Figure 2
Optimized geometry of the indolylquinones through B3LYP/6-311++G(d,p).Molecular Geometries. The calculated geometrical parameters of6–9 are displayed in Tables 2 and 3. Since the target molecules have no experimental X-rays partners and considering the important motif in the biological activities, the experimental data of the starter substrates and similar indol derivative present in the literature [40–42] was compared with the predicted value for the target molecules.Table 2
Theoretical and experimental selected bond length (in angstrom) of6 and 7.
Bond
6
7
Theoretical length
Experimental length
Theoretical length
Experimental length
C1-O1
1.223
1.226a
1.228
1.240c
C1-C2
1.480
1.469a
1.489
1.450c
C2-C3
1.351
1.334a
1.359
1.350c
C3-C4
1.494
1.498a
1.523
1.490c
C1-C6
1.527
1.469a
1.491
1.430c
C3/C6-O
1.343
1.347a
1.340
1.250c
C4-O4
1.231
1.220a
1.221
1.180c
C2-C8
1.518
1.499a
1.527
1.500c
C4-C5
1.467
1.472a
1.474
1.430c
C5-C7
1.503
1.507a
1.499
1.580c
C=O⋯H
1.965
2.340a
1.985
2.650c
C5-C6
1.362
1.332a
1.348
1.390c
C6/C3-C3′
1.461
1.448b
1.475
1.448b
C2′-C3′
1.382
1.375b
1.373
1.375b
C2′-N
1.368
1.363b
1.377
1.363b
C8′-N
1.389
1.370b
1.382
1.370b
aObtained from Soriano-García et al., 1986 [40]; bobtained from Noltemeyer et al., 1982 [41]; cobtained from Enríquez et al., 1998 [42].Table 3
Theoretical and experimental selected bond length (in Å) of8 and 9.
Bond
8
9
Theoretical length
Experimental length
Theoretical length
Experimental length
C1=O1
1.223
1.229a
1.223
1.228c
C1-C2
1.494
1.453a
1.491
1.451c
C2-C3
1.363
1.366a
1.365
1.306c
C3-C4
1.503
1.484a
1.498
1.470c
C3-C3′
1.471
1.448a
1.467
1.448c
C4=O4
1.219
1.231a
1.240
1.229c
C2-C11
1.503
1.491a
1.501
1.525c
C4-C10
1.492
1.467a
1.465
1.460c
C10-C5
1.397
1.392a
1.415
1.460c
C10-C9
1.400
1.401a
1.405
1.386c
C5-C6
1.391
1.398a
1.384
1.318c
C6-C7
1.398
1.387a
1.400
1.405c
C7-C8
1.391
1.389a
1.387
1.400c
C8-C9
1.397
1.398a
1.410
1.364c
C9-C1
1.488
1.479a
1.49
1.492c
C5-HO5
—
—
1.339
1.320c
C=O⋯H
—
—
1.696
1.870c
C2′-C3′
1.376
1.375b
1.379
1.375b
C2′-N
1.374
1.363b
1.370
1.363b
C8′-N
1.383
1.370b
1.383
1.370b
aObtained from Nowell and Attfield, 2004 [43]; bobtained from Noltemeyer et al., 1982 [41]; cobtained from Vijayalakshmi et al., 1987 [44].Table2 shows the bond length of6 and7 by similar structure in both molecules. In the case of6 compared with perezone quinonic ring, the predicted values are very similar to1. However, the C1-C6 bond in6 is longer in comparison with the perezone. Additionally, the hydrogen bond present between the O3⋯H hydroxyl group and the O4 carbonyl group decreases the distance, signaling a more stronger bond (intramolecular hydrogen bond); the O3⋯O4 and H3⋯O4 distances are 2.583 and 1.965 Å beside O3-H3⋯O4 angle of 118.839°; these data agree with those of Soriano-García et al. [40]. Considering the indolyl moiety, the values were compared with the experimental values obtained for the X-ray diffraction of 2-ethyl-5-(3-indolyl)oxazole [41].The contrast of the indolyl fragment of6 with the reference shows very similar values in both molecules; however, the predicted bonds are longer in6.The predicted values of7 compared with the isoperezone show a similar trend in the quinonic ring; however, the bonds C3-C4 and C1-C6 are longer than in the experimental case. In the same sense, the intramolecular hydrogen bond is shorter, indicating the increase of the strength in this bond.Regarding the indolyl moiety in7, the estimated values are in good agreement with the experimental values considering the structural difference between7 and the 2-ethyl-5-(3-indolyl)oxazole.Table3 summarizes some theoretical values of bond length for8 and9 contrasted with the X-rays values of3 and4 [43, 44] and the indolyl structure with 5-(3-indolyl) oxazole [41]. The calculated values for the bond C3-C4 of8 and9 are larger and the hydrogen bond of9 is shorter following the same trends as the previous compounds (3 and4). The rest of the theoretical values are coherent and near the experimental values, appropriate with the theory level used.The enlargement of the bonds C1-C6 (for6 and7) and C3-C4 (for7,8, and9) in the quinonic rings and shortening in the intramolecular hydrogen bond (6,7, and9) can be explained by the resonance effect promoted by the addition of the indolic structure. The electrons of indole moiety can produce greater localization of electrons in the carbonyl group oxygen (C4=O4 in6 and C1=O1 in7,8, and9) in contrast to1–4 in which the double bonds of the quinone ring can be delocalized to both carbonyl groups. The consequence of this effect in6–9 is the major negative charge in the oxygen atom in the previous carbonyl group signaled and therefore greater attractive force to hydrogen and minor electronic delocalization to the bonds C1-C6 and C3-C4, provoking enlargement of these bonds.NMR Parameters. The experimental values corresponding to the chemical shifts of 1H and 13C were correlated with the calculated values by DFT at the B3LYP and using the 6-311++G(d,p) basis set and the GIAO method.The calculated GIAO1H NMR chemical shifts were plotted versus the experimental data obtained for the indolylquinones (Figure 3(a)).Figure 3
Linear regression between experimental and calculated B3LYP/6-311++G(d,p): (a)1H NMR chemical shifts and (b) 13C NMR chemical shifts for the indolylquinones obtained in this work.
(a)
(b)Linear regression analysis of the data set of1H NMR shifts provided the following results and presented a regression coefficient of 0.9806 and standard deviation of 0.3930 ppm; the equation to describe the fit is δ
T = 0.9875δ
e
x  +  0.1661 ppm, where δ
T is a chemical shift predicted in the base of the experimental values δ
e
x, with the slope and intercept having a standard deviation of 0.0196 and 0.1261, respectively.The same analysis was made for the theoretical and experimental chemical shifts values obtained for13C NMR; the corresponding plot is shown in Figure 3(b); in this regard, the linear regression analysis for 13C chemical shifts yielded a regression coefficient of 0.9955 and standard deviation of 3.35 ppm; the equation to describe the fit is δ
T = 1.0297δ
e
x  +  3.138 ppm, where the slope and intercept showed a standard deviation of 0.0076 and 0.937, respectively.In other words, the values of the regression coefficients in both analyses reflected a good description of the experimental chemical shifts of1H and 13C NMR by the selected method, theory level, and basis set.


## 3.1. Synthesis

The reactions of1–4 with indole (5), under solventless conditions and with TAFF as a catalyst, were performed using the following three nonconventional modes of activation: microwave and near-infrared irradiation and high speed ball milling, affording the respective indole derivatives6–9 (Scheme 1); in addition, the target molecules were comparatively obtained using mantle heating conditions; the corresponding results are summarized in Table 1.Table 1
Production of indolylquinones derivatives, using different activating modes.
Product
Yields %
MH
MW
NIR
HSBM
6
24
79
62
24
7
12
35
31
18
8
46
42
51
30
9
25
41
64
35
Best reaction conditions: MH, 95°C and 60 min; MW, 250 W, 100°C, and 10 min; NIR, 121°C and 10 min; HSBM, 500 rpm and 60 min.Scheme 1
Synthesis of indole-quinone derivatives.In general, the pathway must occur by classical Michael addition by the indol to the corresponding quinonic substrates, assisted by an interaction of the oxygen of a carbonylic group with the Lewis acidic sites of TAFF, followed by in situ oxidation, producing6–9 in moderated yields at low times. It is appropriate to note that6,7, and9 are novel molecules, and according to our knowledge8 has been previously produced by MH and US in the presence of solvent with the employment of a noninnocuous catalyst [28, 55, 56]. Moreover, literature research shows that this seems to be the first report for the green production of indolylquinones using MW, NIR, and HSBM as the activating modes without a solvent and in the presence of a natural ecofriendly catalyst.A complementary and interesting result was the transformation of perezone into isoperezone, using for the first time HSBM (Scheme2), in comparative yields and reaction times, with values previously reported [9, 12].Scheme 2
Transformation of perezone into isoperezone.For the structural attribution of2 and6–9, in a first instance, isoperezone was spectroscopically correlated with literature data [9] as well as its melting point (108-109°C). Considering the notion that6,7, and9 are novel products and that they are structurally related to8, appropriate spectroscopic characterizations of the target molecules were performed. Thus, in a first place, the corresponding accurate molecular ions were acquired by HRMS-EI to achieve the elemental compositions; this was obtained in agreement with the expected products.In regard to the1H NMR, it is worth noting the disappearance of the corresponding signals assigned to H-3 of indole and reagents2–4, in addition to the signal assigned to H-6 of perezone; moreover, the signal due to the proton linked to the nitrogen of the indole, δ 7.81 ppm, was displaced, for6–9 to lower fields 8.4–8.9 ppm, unequivocally due to deprotection generated by the high degree of conjugation between the indolic-quinonic moieties of the products. In this sense, the obtained 13C NMR data complemented the structural characterization of molecules6–9, and the signals of C-3 and C-2 of indole at 102.22 ppm and 111.10 ppm were, respectively, displaced to lower fields, 107.9–108.9 and 122.3–128.2 ppm, respectively. This change in the chemical shift was also attributed to deprotection by the quinonic system. In general, the 1H and 13C NMR experimental data correlated appropriately with those values predicted by computational models.


## 3.2. Cytotoxic Activity

Indolylquinones cytotoxicities were evaluated in human MDA-MB-231 breast cancer cells using the MTT assay, employing as control normal fibroblast cells. The studied compounds were assayed in concentrations of 0 to 100μg/mL for6, 0 to 150 μg/mL for7, 0 to 110 for8, and finally 0 to 130 μg/mL for9. According to Figure 1, the decrease of viable cells starts at 24 hours of incubation of the studied compounds; moreover molecule7 began to decrease at a lower concenttration (15 mg/mL); on the other hand, for the rest of the molecules, the decrease of the viability began at concentrations higher than 20 μg/mL. The same behavior was exhibited at 48 h with cancer cell viability in the range from 10 to 20% when the highest concentrations of 100, 150, 110, and 130 μg/mL to6,7,8, and9, respectively, were used. The results after 72 h showed in7 and9 similar trends to those at 48 h; however,6 and8 increased the cellular viability in the highest concentrations.The IC50 values were obtained through extrapolation from the dose-response graphs (Figure 1). The results of the viability in human breast cancer cells against the four molecules tested recognize7 as the most active molecule with IC50 of 25 μg/mL. In addition, for6,8, and9, the values of IC50 were 40.6, 41.6, and 32.3 μg/mL, respectively. These concentrations were tested against healthy dermal fibroblast. With this result, it is important to point out that only9 developed a cytotoxic effect with 15% mortality; on the other hand,6–8, instead of promoting cells death, caused fibroblast proliferation. The effect in healthy cells is very interesting and is currently under study by our research group, as the cytotoxic pathway presented for these compounds.


## 3.3. Computational Study

The molecules obtained were also studied through computational chemistry methods. In the optimization of the target molecules, a conformer distribution and the selection of the most stable conformer were achieved. Additionally, the selected geometries were appropriately optimized by DFT employing B3LYP functional and the 6-311++G(d,p) basis set. The structures obtained in phase gas are depicted in Figure2.Figure 2
Optimized geometry of the indolylquinones through B3LYP/6-311++G(d,p).Molecular Geometries. The calculated geometrical parameters of6–9 are displayed in Tables 2 and 3. Since the target molecules have no experimental X-rays partners and considering the important motif in the biological activities, the experimental data of the starter substrates and similar indol derivative present in the literature [40–42] was compared with the predicted value for the target molecules.Table 2
Theoretical and experimental selected bond length (in angstrom) of6 and 7.
Bond
6
7
Theoretical length
Experimental length
Theoretical length
Experimental length
C1-O1
1.223
1.226a
1.228
1.240c
C1-C2
1.480
1.469a
1.489
1.450c
C2-C3
1.351
1.334a
1.359
1.350c
C3-C4
1.494
1.498a
1.523
1.490c
C1-C6
1.527
1.469a
1.491
1.430c
C3/C6-O
1.343
1.347a
1.340
1.250c
C4-O4
1.231
1.220a
1.221
1.180c
C2-C8
1.518
1.499a
1.527
1.500c
C4-C5
1.467
1.472a
1.474
1.430c
C5-C7
1.503
1.507a
1.499
1.580c
C=O⋯H
1.965
2.340a
1.985
2.650c
C5-C6
1.362
1.332a
1.348
1.390c
C6/C3-C3′
1.461
1.448b
1.475
1.448b
C2′-C3′
1.382
1.375b
1.373
1.375b
C2′-N
1.368
1.363b
1.377
1.363b
C8′-N
1.389
1.370b
1.382
1.370b
aObtained from Soriano-García et al., 1986 [40]; bobtained from Noltemeyer et al., 1982 [41]; cobtained from Enríquez et al., 1998 [42].Table 3
Theoretical and experimental selected bond length (in Å) of8 and 9.
Bond
8
9
Theoretical length
Experimental length
Theoretical length
Experimental length
C1=O1
1.223
1.229a
1.223
1.228c
C1-C2
1.494
1.453a
1.491
1.451c
C2-C3
1.363
1.366a
1.365
1.306c
C3-C4
1.503
1.484a
1.498
1.470c
C3-C3′
1.471
1.448a
1.467
1.448c
C4=O4
1.219
1.231a
1.240
1.229c
C2-C11
1.503
1.491a
1.501
1.525c
C4-C10
1.492
1.467a
1.465
1.460c
C10-C5
1.397
1.392a
1.415
1.460c
C10-C9
1.400
1.401a
1.405
1.386c
C5-C6
1.391
1.398a
1.384
1.318c
C6-C7
1.398
1.387a
1.400
1.405c
C7-C8
1.391
1.389a
1.387
1.400c
C8-C9
1.397
1.398a
1.410
1.364c
C9-C1
1.488
1.479a
1.49
1.492c
C5-HO5
—
—
1.339
1.320c
C=O⋯H
—
—
1.696
1.870c
C2′-C3′
1.376
1.375b
1.379
1.375b
C2′-N
1.374
1.363b
1.370
1.363b
C8′-N
1.383
1.370b
1.383
1.370b
aObtained from Nowell and Attfield, 2004 [43]; bobtained from Noltemeyer et al., 1982 [41]; cobtained from Vijayalakshmi et al., 1987 [44].Table2 shows the bond length of6 and7 by similar structure in both molecules. In the case of6 compared with perezone quinonic ring, the predicted values are very similar to1. However, the C1-C6 bond in6 is longer in comparison with the perezone. Additionally, the hydrogen bond present between the O3⋯H hydroxyl group and the O4 carbonyl group decreases the distance, signaling a more stronger bond (intramolecular hydrogen bond); the O3⋯O4 and H3⋯O4 distances are 2.583 and 1.965 Å beside O3-H3⋯O4 angle of 118.839°; these data agree with those of Soriano-García et al. [40]. Considering the indolyl moiety, the values were compared with the experimental values obtained for the X-ray diffraction of 2-ethyl-5-(3-indolyl)oxazole [41].The contrast of the indolyl fragment of6 with the reference shows very similar values in both molecules; however, the predicted bonds are longer in6.The predicted values of7 compared with the isoperezone show a similar trend in the quinonic ring; however, the bonds C3-C4 and C1-C6 are longer than in the experimental case. In the same sense, the intramolecular hydrogen bond is shorter, indicating the increase of the strength in this bond.Regarding the indolyl moiety in7, the estimated values are in good agreement with the experimental values considering the structural difference between7 and the 2-ethyl-5-(3-indolyl)oxazole.Table3 summarizes some theoretical values of bond length for8 and9 contrasted with the X-rays values of3 and4 [43, 44] and the indolyl structure with 5-(3-indolyl) oxazole [41]. The calculated values for the bond C3-C4 of8 and9 are larger and the hydrogen bond of9 is shorter following the same trends as the previous compounds (3 and4). The rest of the theoretical values are coherent and near the experimental values, appropriate with the theory level used.The enlargement of the bonds C1-C6 (for6 and7) and C3-C4 (for7,8, and9) in the quinonic rings and shortening in the intramolecular hydrogen bond (6,7, and9) can be explained by the resonance effect promoted by the addition of the indolic structure. The electrons of indole moiety can produce greater localization of electrons in the carbonyl group oxygen (C4=O4 in6 and C1=O1 in7,8, and9) in contrast to1–4 in which the double bonds of the quinone ring can be delocalized to both carbonyl groups. The consequence of this effect in6–9 is the major negative charge in the oxygen atom in the previous carbonyl group signaled and therefore greater attractive force to hydrogen and minor electronic delocalization to the bonds C1-C6 and C3-C4, provoking enlargement of these bonds.NMR Parameters. The experimental values corresponding to the chemical shifts of 1H and 13C were correlated with the calculated values by DFT at the B3LYP and using the 6-311++G(d,p) basis set and the GIAO method.The calculated GIAO1H NMR chemical shifts were plotted versus the experimental data obtained for the indolylquinones (Figure 3(a)).Figure 3
Linear regression between experimental and calculated B3LYP/6-311++G(d,p): (a)1H NMR chemical shifts and (b) 13C NMR chemical shifts for the indolylquinones obtained in this work.
(a)
(b)Linear regression analysis of the data set of1H NMR shifts provided the following results and presented a regression coefficient of 0.9806 and standard deviation of 0.3930 ppm; the equation to describe the fit is δ
T = 0.9875δ
e
x  +  0.1661 ppm, where δ
T is a chemical shift predicted in the base of the experimental values δ
e
x, with the slope and intercept having a standard deviation of 0.0196 and 0.1261, respectively.The same analysis was made for the theoretical and experimental chemical shifts values obtained for13C NMR; the corresponding plot is shown in Figure 3(b); in this regard, the linear regression analysis for 13C chemical shifts yielded a regression coefficient of 0.9955 and standard deviation of 3.35 ppm; the equation to describe the fit is δ
T = 1.0297δ
e
x  +  3.138 ppm, where the slope and intercept showed a standard deviation of 0.0076 and 0.937, respectively.In other words, the values of the regression coefficients in both analyses reflected a good description of the experimental chemical shifts of1H and 13C NMR by the selected method, theory level, and basis set.


## 4. Conclusions

The methodology employed proceeded with a good green approach [57]; it is in agreement with the followingGreen Chemistry Principles: prevention (principle 1), atom economy (principle 2), less hazardous chemical synthesis (principle 3), safer solvents and auxiliaries (principle 5), design for energy efficiency (principle 6), catalysis (principle 9),andinherently safer chemistry for accident prevention (principle 12). In addition, it is convenient to note that the obtained molecules have a cytotoxic effect in breast cancer cells without apparently toxic effect in healthy cells, and the most cytotoxic molecule (7) corresponds to the minor gap energy (theoretical calculations); and it is also important to mention that the experimental activity will be explained on the base of theoretical studies under the course by our research group.Finally, taking into account the fact that three of the molecules are new, appropriate spectroscopic characterizations were performed by means of1H NMR, 13C NMR, EIMS, and HRMS, and it is important to highlight that the experimental NMR spectroscopic data of the target compounds showed good agreement with theoretical characterization.



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*Source: 3870529-2016-07-20.xml*