# Indole Alkaloids from Marine Sources as Potential Leads against Infectious Diseases

Authors: Paulo H. B. França; Daniel P. Barbosa; Daniel L. da Silva; Êurica A. N. Ribeiro; Antônio E. G. Santana; Bárbara V. O. Santos; José M. Barbosa-Filho; Jullyana S. S. Quintans; Rosana S. S. Barreto; Lucindo J. Quintans-Júnior; João X. de Araújo-Júnior
Journal: BioMed Research International
 (2014)

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## Abstract

Indole alkaloids comprise a large and complex class of natural products found in a variety of marine sources. Infectious diseases remain a major threat to public health, and in the absence of long-term protective vaccines, the control of these infectious diseases is based on a small number of chemotherapeutic agents. Furthermore, the emerging resistance against these drugs makes it urgently necessary to discover and develop new, safe and, effective anti-infective agents. In this regard, the aim of this review is to highlight indole alkaloids from marine sources which have been shown to demonstrate activity against infectious diseases.


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## Body

## 1. Introduction

Seas and oceans occupy more than 75% of the Earth’s surface and contain nearly all groups of organisms, including representatives of 34 out of 36 phyla described. Thus, marine ecosystems can be considered as having the greatest phyletic biodiversity with virtually unlimited biotechnological potential [1]. The marine environment is massively complex, consisting of extreme variations in pressure, salinity, temperature, and biological habitats that have led to the production of several novel structures with unique biological properties, which may not be found in terrestrial natural products [2]. In the past 30–40 years, marine plants and animals have been the focus of a worldwide effort to define the natural products of the marine environment. A small number of marine plants, animals, and microorganisms have already yielded more than 12,000 novel chemicals, with hundreds of new compounds still being discovered every year. These discovery efforts have yielded several bioactive metabolites that have been successfully developed by the pharmaceutical industry [3]. A variety of marine sources including sponges, tunicates, red algae, acorn worms, and symbiotic bacteria have been shown to generate indole alkaloids, which represent the largest number and most complicated of the marine alkaloids [4–7]. The alkaloids obtained from marine organisms frequently possess novel frameworks which cannot be found in terrestrially related organisms. Marine metabolites often possess complexities such as halogen substituents [8]. In addition, bearing in mind that several species of organisms are associated with cyanobacteria and bacteria, it is considered that several natural products originating in microorganisms can be isolated from marine animals [9]. Indole alkaloids have been shown to exhibit a wide array of biological activities such as opioid receptor agonistic [10], antibacterial [11, 12], antifungal [13, 14], anti-inflammatory [15], antileishmanial [16, 17], antiplasmodial [18, 19], anti-HIV [20], cytotoxic [21], glucose uptake stimulatory [22], larvicidal [23], trypanocidal [24], and vasodilator [25, 26] and inhibition of cholinesterase [27], indoleamine-2, 3-dioxygenase [28], calmodulin [29], and CB1 cannabinoid receptor [30]. Infectious diseases caused by bacteria, fungi, viruses, and parasites are still a major threat to public health, despite the tremendous progress in human medicine. The high prevalence of these diseases and the emergence of widespread drug resistance developed by these parasites to current treatments, leading to reduction of their efficacy and consequent increase in the cost of conventional treatments, highlight the need for novel and effective therapeutic alternatives with fewer or no side-effects. Their impact is particularly large in developing countries due to the relative unavailability of medicines [31, 32].Therefore, in continuation of our research on bioactive molecules from natural origin [33–53] and plant extracts [54–63] we offer this compilation of the indole alkaloids from marine sources.In this review, literature was covered in order to highlight alkaloidal compounds with an indole moiety which have been shown to demonstrate activity against infectious diseases.


## 2. Indole Alkaloids from Marine Sources

Manadomanzamines A and B (Figure1), β-carbolines with a novel rearrangement of the manzamine framework, were isolated from an Indonesian spongeAcanthostrongylophora sp. These compounds showed significant activities againstMycobacterium tuberculosis, with MIC values of 1.9 and 1.5 μg/mL, respectively. Manadomanzamines A and B were also active against human immunodeficiency virus (HIV-1) with EC50 values of 7.0 and 16.5 μg/mL. Furthermore, manadomanzamine A was active against the fungusCandida albicans with an IC50 of 20 μg/mL, whilst manadomanzamine B exhibited activity against the fungusCryptococcus neoformans with IC50 value of 3.5 μg/mL [64].Figure 1
Structures ofβ-carbolines with manzamine-type frameworks.Manzamine A, aβ-carboline alkaloid present in several marine sponge species, has been shown to inhibit the growth of the rodent malaria parasitePlasmodium berghei in vivo. More than 90% of the asexual erythrocytic stages ofP. berghei were inhibited after a single intraperitoneal injection of manzamine A into infected mice. Moreover, it was demonstrated that immunostimulatory effects caused by the compound play an important role in preventing mouse death due to fulminating recurrent parasitemia in animals treated with 100 mmol per kg of manzamine A [65].Several manzamine-type alkaloids were also isolated from an Indonesian sponge of the genusAcanthostrongylophora. The β-carbolines (+)-8-hydroxymanzamine A and 6-hydroxymanzamine E (Figure 1) showed strong activity againstM. tuberculosis H37Rv with MIC values of 0.9 and 0.4 μg/mL, respectively. Manzamine A and (+)-8-hydroxymanzamine were strongly active when tested againstPlasmodium falciparum chloroquine-sensitive D6 clone and chloroquine-resistant W2 clone, showing, respectively, IC50 values of 4.5 and 6.0 ng/mL toward the former and equally 8.0 ng/mL toward the latter. Moreover, manzamine A, manzamine Y, and 6-hydroxymanzamine E (Figure 1) have shown significant activity againstLeishmania donovani, with IC50 values of 0.9, 1.6, and 2.5 μg/mL, respectively [66].Investigation of bioactivity of manzamine alkaloids isolated from an Okinawan spongeAmphimedon sp. revealed active compounds againstTrypanosoma brucei brucei andP. falciparum.Zamamidine C (Figure 1), 3,4-dihydromanzamine JN-oxide, and manzamine A showed inhibitory activitiesin vitro againstT. b. brucei with IC50 values of 0.27, 4.44, and 0.04 μg/mL, respectively, andP. falciparum with IC50 values 0.58, 7.02, and 0.97 μg/mL, respectively [67].Sevenβ-carboline-based metabolites, designated as eudistomins Y1–Y7 (Figure 2), were isolated from a tunicate of the genusEudistoma collected in South Korea. These new metabolites differ from previously isolated marine metabolites due to the presence of a benzoyl group attached to the β-carboline nucleus at C-1. Eudistomin Y6 exhibited moderate antibacterial activity against Gram-positive bacteriaStaphylococcus epidermidis ATCC12228 andB. subtilis ATCC 6633 with MIC values of 12.5 and 25 μg/mL, respectively, but showed no inhibitory activity toward the other two strains of Gram-positive bacteria,S. aureus ATCC 6538 andM. lutes ATCC 9341, and the Gram-negative bacteria includingE. coli ATCC 11775,Salmonella typhimuriumATCC 14028, andKlebsiella pneumoniae ATCC 4352. Eudistomins Y1 and Y4 also displayed the same selectivity as eudistomin Y6 but demonstrated weak antibacterial activity against the two strains of bacteriaS. epidermidis ATCC12228 andB. subtilis ATCC 6633 with MICs of 50 and 200 μg/mL, respectively [68].Figure 2
Structures ofβ-carbolines with eudistomin-derived scaffold and simple β-carbolines.Investigation of theCH2Cl2 extract from the bryozoanPterocella vesiculosa, collected in New Zealand, has led to the isolation of 5-bromo-8-methoxy-1-methyl-β-carboline (Figure 2). This alkaloid was evaluated for antibacterial and antifungal activities and showed inhibitory action toward the Gram-positive bacteriumB. subtilis and the fungiC. albicans andTrichophyton mentagrophytes with MID ranges of 2–4, 4-5 and 4-5 μg/mL, respectively [69].An indole spermidine alkaloid, didemnidine B (Figure3), was described in the New Zealand ascidianDidemnum sp. Evaluation of the compound againstTrypanosoma brucei rhodesiense,Trypanosoma cruzi,L. donovani, andPlasmodium falciparum K1 chloroquine-resistant strain indicated didemnidine B to be mildly active toward the malaria parasite with IC50 value of 15 μM [70].Figure 3
Structures of simple indole alkaloids.In addition, chemical investigations of the tropical marine spongeHyrtios sp. have resulted in the isolation of several alkaloids, which were evaluated asC. albicans isocitrate lyase inhibitors. Out of the compounds tested, the bis-indole alkaloid hyrtiosin B (Figure 4) showed the most potent inhibitory activity with an IC50 value of 50.7 μM. Other compounds comprised simple indole alkaloids, namely, 1-carboxy-6-hydroxy-3,4-dihydro-β-carboline, 5-hydroxy-1H-indole-3-carboxylic acid methyl ester, serotonin, hyrtiosin A, and 5-hydroxyindole-3-carbaldehyde (Figure 3) revealed only moderate to weak activity againstC. albicans isocitrate lyase, with MIC values ranging between 39.8 and 152.9 μM. By comparing their chemical structures, it was found that the enzyme inhibitory activities of these 5-hydroxyindole-type alkaloids are markedly affected by a substitution of functional group at the C-3 position. A substitution by a hydrophilic group at the C-3 position results in an increase of the isocitrate lyase inhibitory activity [71].Figure 4
Structures of bis-indole alkaloids.Tryptophol (Figure3), a simple indole alkaloid from spongeIrcinia spinulosa, was screened for antitrypanosomal activity. The compound was active againstT. b. rhodesiense with an IC50 value of 5.89 μg/mL, but it was 8-fold less active againstT. cruzi, with IC50 value of 49.37 μg/mL, than againstT. b. rhodesiense. TowardL. donovani, tryptophol displayed an IC50 value of 9.60 μg/mL, compared with 0.20 μg/mL of the standard drug, miltefosine. ForP. falciparum, it was shown that tryptophol presented an IC50 value of 5.08 μg/mL, compared with 0.056 μg/mL of chloroquine [72].A bioactive bromine-containing oxindole alkaloid, matemone (Figure3), was isolated from the Indian Ocean spongeIotrochota purpurea. Matemone showed marginal antimicrobial activity against the bacteriumStaphylococcus aureus at 50, 100, and 200 μg/disk, affording inhibitory zones of 7, 9, and 11 mm, respectively [73].Dendridine A (Figure4), a C2-symmetrical 4,4′-bis(7-hydroxy) indole alkaloid, was reported in extracts of an Okinawan spongeDictyodendrilla sp. Dendridine A exhibited inhibitory activities against Gram-positive bacteriaBacillus subtilis andMicrococcus luteus with MIC values of 8.3 and 4.2 μg/mL, respectively, and the fungusC. neoformans with MIC of 8.3 μg/mL [74].In order to discover active compounds with inhibitory activity against methicillin-resistantS. aureus pyruvate kinase (MRSA-PK), screening of an extract library of marine invertebrates resulted in the identification of bis-indole alkaloids from theTopsentia pachastrelloides. The most active compounds,cis-3,4-dihydrohamacanthin B and bromodeoxytopsentin (Figure 4), were identified as highly potent MRSA-PK inhibitors with IC50 values of 16–60 nM and with at least 166-fold selectivity over human PK isoforms. These novel anti-PK natural compounds exhibited significant antibacterial activities against MRSA with MIC values of 12.5 and 6.25 μg/mL, respectively, and selectivity indices (CC50/MIC) > 4 [75].The bis-indole alkaloid nortopsentin A (Figure4) present in enriched fractions of marine sponges from genusSpongosorites exhibited potent inhibition ofPlasmodium falciparum growth. Assays were performed in chloroquine-sensitive (3D7) and chloroquine-resistant (Dd2) strains, and IC50 values obtained were 460 nM against the former and 560 nM against the latter [76].The bisindole alkaloid (R)-6′-debromohamacanthin B (Figure 4) was isolated from the MeOH extract of a marine spongeSpongosorites sp. and showed weak antibacterial activity against clinically isolated methicillin-resistant strains, with MIC values of 6.3 μg/mL forS. pyogenes 308A and 12.5 μg/mL forS. pyogenes 77A,S. aureus SG 511;S. aureus 285;S. aureus 503 [77].Bis-indole alkaloids isolated from the Jamaican spongeSmenospongia aureawere tested against the D6 clone ofP. falciparum for theirin vitro antimalarial activity. The compound 6-bromoaplysinopsin (Figure 4) exhibited activity at endpoints of 0.087 and 0.34 μg/mL with a selectivity index of 55 and 14, respectively. Other compounds such as isoplysin A and 6-bromo-2′-de-N-methylaplysinopsin (Figure 3) showed moderate activity at 0.97, 1.1, and 0.94 μg/mL with a selectivity index of >4.9, >4.3, and >5.1, respectively. Moreover, 6-bromo-2′-de-N-methylaplysinopsin inhibited the antimalarial target plasmepsin II enzyme with IC50 53 μM in fluorescence resonance energy transfer (FRET) and 66 μM in fluorescence polarization (FP) assays [78].Bioassay-guided fractionation of the 7 : 3 MeOH/water extract of a cultured cyanobacterium strain identified asFischerella sp. yielded nine isonitrile-containing alkaloids. Ambiguine H isonitrile and ambiguine I (Figure 5) isonitrile exhibited strong activity against the bacteriaStaphylococcus albus(MIC values of 0.625 and 0.078 μg/mL) andB. subtilis (MIC values of 1.25 and 0.312 μg/mL). Ambiguine I isonitrile was strongly active against the fungiSaccharomyces cerevisiae (MIC value of 0.312 μg/mL) andC. albicans ATCC 90028 (MIC value of 0.39 μg/mL) [79].Figure 5
Structures of ambiguine isonitriles.A new investigation on ambiguines highlighted that ambiguine A isonitrile showed MIC of 1.0μM againstB. anthracis, while ambiguine K and M isonitriles (Figure 5) were more potent towardsM. tuberculosis with MIC values of 6.6 μM and 7.5 μM, respectively [80].From the CH2Cl2 extract of the spongeHyrtios cf.erecta, collected in Fiji, β-carbolines homofascaplysin A and fascaplysin (Figure 6) were isolated. Evaluation of the biological activity of the compounds towardP. falciparum revealed that homofascaplysin and fascaplysin are both potently activein vitro against the parasite. Homofascaplysin A also inhibited the growth ofEscherichia coli(50 μg/9 mm) andBacillus megaterium(50 μg/11 mm). Fascaplysin inhibited the growth ofE. coli (50 μg/6 mm) andB. megaterium (50 μg/10 mm). Further biological activity for fascaplysin was found againstTrypanosoma b. rhodesiense, displaying moderate activity with IC50 value of 0.17 μg/mL compared with melarsoprol, which showed IC50 value of 2 ng/mL. The evaluation of antiviral activity of fascaplysin revealed an increased cytopathogenic effect at noncytotoxic concentrations of 0.038 μg/mL toward fetal Rhesus monkey kidney cells (FRhK-4-cells) infected with the HAV-variant HAVcytHB1.1. Persistently infected FRhK-4-cells (HAV/7) showed cytopathogenicity at 0.038 μg/mL of fascaplysin, while untreated FRhK-4-cells remained unchanged. Homofascaplysin A and fascaplysin were shown to be potentin vitro inhibitors of chloroquine-susceptible (NF54) and chloroquine-resistantP. falciparumstrains. Positive control substances were chloroquine and artemisinin. The potency against the K1 strain of homofascaplysin A was stronger than that of chloroquine. Compared with artemisinin (K1 strain) and with both positive control substances (NF54 strain), homofascaplysin was approximately 10-fold less active [81].Figure 6
Structures of fascaplysin and homofascaplysin A.Alkaloids obtained from the fermentation broth ofMarinactinospora thermotolerans SCSIO 0652 were tested for their antiplasmodial activities againstP. falciparum line 3D7, a drug-sensitive strain, and Dd2, a multi-drug-resistant strain. The results of the antiplasmodial assays revealed that marinacarboline A (Figure 7) and methylpendolmycin-14-O-α-glucoside (Figure 6) inhibitedP. falciparum line Dd2 with IC50 values of 1.92 and 5.03 μM, respectively, and marinacarbolines C and D inhibitedP. falciparum lines 3D7 and Dd2 with IC50 values between 3.09 and 5.39 μM [82].Figure 7
Structures of marinacarbolines and pendolmycin derivative.The cultivation ofStreptomyces sp. strains associated with the Mediterranean spongesAplysina aerophoba,Axinella polypoides,Tedania sp., andTethya sp., collected in Croatia, has yielded the indolocarbazole alkaloid staurosporine (Figure 8). The compound was screened for anti-infective activities and showed significant antiparasitic activity againstLeishmania major with IC50 5.30 μM andT. b. brucei with IC50 0.022 μM [83]. Staurosporine was first isolated in 1977 fromStreptomyces staurisporeus and later also from other actinomycetes as well as cyanobacteria. In the meantime several staurosporine analogues were isolated from actinomycetes and also from marine invertebrate, including among others sponges, mollusks, and tunicates. Interestingly, in several cases staurosporine and related derivatives were isolated from Streptomyces sp. The occurrence of such compound may suggest the presence of associated microorganisms responsible for the biosynthesis of staurosporine [84].Figure 8
Structure of indolocarbazole alkaloid staurosporine.A novel indole alkaloid containing pyrazinoquinazoline-derivative framework, (14S)-oxoglyantrypine (Figure 9), isolated from the culture of the mangrove-derived fungusCladosporium sp. PJX-41 exhibited activity against influenza virus H1N1 with IC50 value of 85 μM [85].Figure 9
Structure of pyrazinoquinazoline-derived alkaloid oxoglyantrypine.From marineAspergillus sp., a prenylated indole alkaloid was isolated and its antibacterial activity was assayed. (−)-Stephacidin A (Figure 10) showed MIC value of 21.5 μM againstS. epidermidis[86].Figure 10
Structure of prenylated indole alkaloids (−)-stephacidin A.Nakijinamines A, B, and C (Figure11) have been shown to present antibacterial and antifungal activity. These compounds present a heteroaromatic aaptamine-type framework and a halogenated indole moiety. Nakijinamine A was active againstC. albicans(IC50 0.25 μg/mL),C. neoformans(IC50 0.5 μg/mL),Trichophyton mentagrophytesIC50 (0.25 μg/mL),S. aureus(MIC 16 μg/mL),B. subtilis(MIC 16 μg/mL), andMicrococus luteus (MIC 2 μg/mL). Nakijinamines B and C were only active againstC. albicans with IC50 value of 8 μg/mL each [87].Figure 11
Structures of heteroaromatic aaptamine-type indole alkaloids.Alkaloids with hyrtimomine-type framework (Figure12) were isolated fromHyrtios spp. Hyrtimomines F, G, and I exhibited inhibitory effects againstAspergillus niger (IC50 8.0 μg/mL each), while hyrtimomine I showed inhibitory effect againstC. neoformans (IC50 4.0 μg/mL). Hyrtimomines A and B also showed antifungal activities againstC. albicans (IC50 1.0 μg/mL each) andC. neoformans (IC50 2.0 and 4.0 μg/mL, resp.). Hyrtimomine A exhibited an inhibitory activity againstA. niger (IC50 4.0 μg/mL) [88].Figure 12
Structures of hyrtimomine-type alkaloids.Meridianins A-G (Figure13) comprise indole alkaloids substituted at the C-3 position by a 2-aminopyridine ring and had been previously isolated from the tunicateAplidium meridianum. Investigations on the antimalarial and antileishmanial activity of meridianin C and G were carried out and showed that these two compounds inhibitedP. falciparum D6 and W2 clones with IC50 values in the range of 4.4 to 14.4 μM. Meridianin C was also active againstL. donovani promastigotes showing an IC50 value of 64 μM [89].Figure 13
Structures of meridianins C and G.Simple indole-derived alkaloids were isolated from the marine bacteriumBacillus pumilus. Compounds 3-hydroxyacetylindole and N-acetyl-β-oxotryptamine (Figure 3) promoted inhibition on the growth of amastigotes fromT. cruzi with IC50 values of 20.6 and 19.4 μM, respectively [90].Hyrtioerectines D–F (Figure14) were isolated fromHyrtios species and had their antimicrobial activities screened. These β-carboline-derived compounds showed inhibition zones of 17, 9, and 14 mm againstC. albicans compared to 35 mm illustrated by clotrimazole at the same concentration. In addition, they caused inhibition zones of 20, 10, and 16 mm againstS. aureus, respectively, compared to 30 mm illustrated by ampicillin, as well as inhibition zones of 7–9 mm againstPseudomonas aeruginosa compared to 30 mm illustrated by imipenem [91].Figure 14
Structures of hyrtioerectines D–F.As noted in the study above, the main source of marine indole alkaloids are sponges, whilst pharmacological assays focus on activity toward parasites from the genusPlasmodium andTrypanosoma. Although few indole alkaloids from marine sources have been tested for anti-infective activity, there are some promising compounds which demonstrate high level of activity against several infectious diseases agents including bacteria, fungi, and protozoa. These active alkaloids show simple as well as complex frameworks and unlike terrestrial organisms, they frequently contain halogenated moieties. Therefore this review highlighted some marine indole alkaloids which may be considered as potential starting points for the development of novel agents for the treatment of infectious diseases.



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