# Isolation and Characterization of Two New Antimicrobial Acids fromQuercus incana (Bluejack Oak)

Authors: Rizwana Sarwar; Umar Farooq; Sadia Naz; Nadia Riaz; Syed Majid Bukhari; Abdur Rauf; Yahia N. Mabkhot; Salim S. Al-Showiman
Journal: BioMed Research International
 (2018)

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## Abstract

Two new compounds[1-2] were purified from ethyl acetate fraction ofQuercus incana. The structure of these compounds is mainly established by using advanced spectroscopic technique such as UV, IR, one-dimensional (ID) and two-dimensional (2D) NMR techniques, and EI mass. The structural formula was deduced to be 4-hydroxydecanoic acid [1] and 4-hydroxy-3-(hydroxymethyl) pentanoic acid [2]. Both isolated compounds were tested for their antimicrobial potential and showed promising antifungal activity againstAspergillus niger andAspergillus flavus.


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## Body

## 1. Introduction

The family Fagaceae is large family comprising 8 genera and about 800–1100 species.Quercus is the largest genus of family Fagaceae having huge medicinal importance and mostly found in dry conditions [1]. The genusQuercus have long been considered among the clades of woody angiosperms in terms of species diversity, horticultural merit, ecological dominance, and industrial and economic values [2]. TheQuercus roburis the only cultivated species while other 600 known species are found in temperate regions of the Northern Hemisphere, Southward through Central America to Colombia and through Turkey to Pakistan [3].The wood is durable, is attractively grained, and is mostly utilized for timber purposes; it is particularly important in shipbuilding, construction for flooring, furniture, railroad ties, and veneers. The bark ofQuercus spp. has been used for medicinal purposes and is an important source of phenolic compounds like tannins which are used for tanning leather and wine production [4]. The fruit (acorn) ofQuercus has husk coating which is edible and highly nutritious and rich in carbohydrates and protein.Quercus (oak) species are utilized in conventional pharmaceutical, as astringent, antiseptic, and hemostatic and in addition to the treatment of acute diarrhea, hemorrhoid, and oral, genital, and anal mucosa inflammation. Moreover, the decoction plants from this genus can be used against burns and added to ointments for the healing of cuts [5]. Oak seeds are a major source of sugar, amino acids, lipids, and different sterols [6].Quercusspecies have been utilized against problems of skin, wounds and gastrointestinal illnesses [7], astringent, mellow germ-free, small cuts [8], and mouth washes [9] all suggesting their antimicrobial potential.GenusQuercus is characterized by six species found mostly in Northern areas of Pakistan. The most promising timber specie isQuercus incana Roxb. (Blue jack oak or cinnamon oak) locally called Ban shindar, Kharpata serci (Punjabi), Rein (Hindko), and Serie (Pushto) [10]. TheQ. incana has huge medicinal usage; it may be used as astringent [11], diuretic, and antidiarrheal agent and for treatment of asthma. Bark and leaves ofQ. incana may be used as antipyretic, antirheumatism, antidiabetic, and antiarthritic purposes [12]. These medical applications and therapeutic potential ofQuercus incana prompted us to carry out the phytochemical investigation to explore biologically active compounds.


## 2. Material and Methods

### 2.1. Experimental Procedures

The ethyl acetate soluble fraction was selected for isolation of bioactive compounds using column chromatographic analysis having column silica and flash silica gel as an adsorbent material. The column was eluted by usingn-hexane and ethyl acetate with increasing polarity, which yield two new compounds 4-hydroxy decanoic acid [1] and 4-hydroxy-3-(hydroxymethyl) pentanoic acid [2] by increasing polarity. The purity of compounds [1-2] was checked by using precoated TLC plates. The IR spectrum was recorded by using spectrophotometer JASCO-320A. The EI mass was recoded on double focusing Varian MAT-312 Spectrometer. 1H-NMR and 13C-NMR spectra were measured by using advance Bruker AMX-300 spectrometer machine. The chemical shifts in parts per million (δ) relative to tetramethylsilane as an internal standard and scalar(J) were described in Hz.


### 2.2. Plant Extraction and Fractionation

Extraction and fractionation ofQuercus incana were reported in our previous study [13]. Ethyl acetate soluble fraction was subjected to repeated column chromatography which yielded two pure compounds [1-2].


### 2.3. Antibacterial Activity of Compounds [1-2]

Antibacterial activity was performed by agar well diffusion method with some modifications [14]. Three Gram-positive (Staphylococcus aureus, Micrococcus luteus,andBacillus subtilis) and Gram-negative (Escherichia coli, Pseudomonas pickettii, andShigella flexneri) pathogens were used in study. 10 µg of each compounds [1-2] was dissolved in 1 mL DMSO. Standard drug and each sample (20 μL) were poured in 6 mm well. The assay plates were incubated at 37°C for 24 hrs. The zone of inhibition was dignified in mm and DMSO was used as a negative control in the experiment.


### 2.4. Antifungal Assay

Disc diffusion methods were used for determination of antifungal effects by using two selected fungal strains such asAspergillus nigerandAspergillus flavus [15]. DMSO was used as a solvent; before applying compounds on petri plates DMSO was completely evaporated.Characterization of Compound 1. Colorless oil; IR (KBr) υmax 3622 br (OH), 1714 (C=O) cm−1. αD25 + 34.80° (c=0.78, CHCl3). EI-MS m/z: (rel. int.) 188 M+ (15), 176 (9), 157 (35), 128 (5), 115 (9). HR-EI-MS: m/z 188. 1420 (calcd. for 188.1412 for C10H20O3). 1H-NMR (CDCl3, 300 MHz): δ 3.62 (1H, m, H-4), 0.88 (3H, t,J = 7.7 Hz, H-10), 2.08 (2H, m, H-2), 1.49, 1.62 (2H, m, H-3), 1.45 (2H, m, H-5), 1.47, 1.33 (2H, m, H-6), 1.30 (2H, m, H-7), 1.28 (2H, m, H-8), 1.29 (2H, m, H-9). 13C-NMR (CDCl3, 75 MHz): δ 179.6 (C-1), 72.1 (C-4), 13.9 (C-10), 34.6 (C-2), 34.9 (C-3), 37.9 (C-5), 26.1 (C-6), 29.8 (C-7), 32.0 (C-8), 22.1 (C-9).Characterization of Compound 2. Colorless oil: IR (KBr) υmax 3495 (OH), 1708 (C=O) cm−1. αD25 + 53.60° (c=0.97, CHCl3). EI-MS m/z: (rel. int.%) 131 M-OH+ 131 (10), 115 (8), 86 (100), 71 (31). HR-EI-MS: m/zM-OH+ 131.0744 (calcd. 131.0736 for C6H11O3-OH). 1H-NMR (CDCl3, 300 MHz): δ 2.35 (1H, m, H-2), 2.04 (1H, m, H-2), 2.58 (1H, m, H-3), 3.92 (1H, m, H-4), 1.23 (3H, d,J = 6.9 Hz, H-5), 4.31 (2H, m, H-1′).13C-NMR (CDCl3, 75 MHz): δ 177.5 (C-1), 27.3 (C-2), 45.9 (C-3), 68.1 (C-4), 20.9 (C-5), 65.5 (C-1′).


## 2.1. Experimental Procedures

The ethyl acetate soluble fraction was selected for isolation of bioactive compounds using column chromatographic analysis having column silica and flash silica gel as an adsorbent material. The column was eluted by usingn-hexane and ethyl acetate with increasing polarity, which yield two new compounds 4-hydroxy decanoic acid [1] and 4-hydroxy-3-(hydroxymethyl) pentanoic acid [2] by increasing polarity. The purity of compounds [1-2] was checked by using precoated TLC plates. The IR spectrum was recorded by using spectrophotometer JASCO-320A. The EI mass was recoded on double focusing Varian MAT-312 Spectrometer. 1H-NMR and 13C-NMR spectra were measured by using advance Bruker AMX-300 spectrometer machine. The chemical shifts in parts per million (δ) relative to tetramethylsilane as an internal standard and scalar(J) were described in Hz.


## 2.2. Plant Extraction and Fractionation

Extraction and fractionation ofQuercus incana were reported in our previous study [13]. Ethyl acetate soluble fraction was subjected to repeated column chromatography which yielded two pure compounds [1-2].


## 2.3. Antibacterial Activity of Compounds [1-2]

Antibacterial activity was performed by agar well diffusion method with some modifications [14]. Three Gram-positive (Staphylococcus aureus, Micrococcus luteus,andBacillus subtilis) and Gram-negative (Escherichia coli, Pseudomonas pickettii, andShigella flexneri) pathogens were used in study. 10 µg of each compounds [1-2] was dissolved in 1 mL DMSO. Standard drug and each sample (20 μL) were poured in 6 mm well. The assay plates were incubated at 37°C for 24 hrs. The zone of inhibition was dignified in mm and DMSO was used as a negative control in the experiment.


## 2.4. Antifungal Assay

Disc diffusion methods were used for determination of antifungal effects by using two selected fungal strains such asAspergillus nigerandAspergillus flavus [15]. DMSO was used as a solvent; before applying compounds on petri plates DMSO was completely evaporated.Characterization of Compound 1. Colorless oil; IR (KBr) υmax 3622 br (OH), 1714 (C=O) cm−1. αD25 + 34.80° (c=0.78, CHCl3). EI-MS m/z: (rel. int.) 188 M+ (15), 176 (9), 157 (35), 128 (5), 115 (9). HR-EI-MS: m/z 188. 1420 (calcd. for 188.1412 for C10H20O3). 1H-NMR (CDCl3, 300 MHz): δ 3.62 (1H, m, H-4), 0.88 (3H, t,J = 7.7 Hz, H-10), 2.08 (2H, m, H-2), 1.49, 1.62 (2H, m, H-3), 1.45 (2H, m, H-5), 1.47, 1.33 (2H, m, H-6), 1.30 (2H, m, H-7), 1.28 (2H, m, H-8), 1.29 (2H, m, H-9). 13C-NMR (CDCl3, 75 MHz): δ 179.6 (C-1), 72.1 (C-4), 13.9 (C-10), 34.6 (C-2), 34.9 (C-3), 37.9 (C-5), 26.1 (C-6), 29.8 (C-7), 32.0 (C-8), 22.1 (C-9).Characterization of Compound 2. Colorless oil: IR (KBr) υmax 3495 (OH), 1708 (C=O) cm−1. αD25 + 53.60° (c=0.97, CHCl3). EI-MS m/z: (rel. int.%) 131 M-OH+ 131 (10), 115 (8), 86 (100), 71 (31). HR-EI-MS: m/zM-OH+ 131.0744 (calcd. 131.0736 for C6H11O3-OH). 1H-NMR (CDCl3, 300 MHz): δ 2.35 (1H, m, H-2), 2.04 (1H, m, H-2), 2.58 (1H, m, H-3), 3.92 (1H, m, H-4), 1.23 (3H, d,J = 6.9 Hz, H-5), 4.31 (2H, m, H-1′).13C-NMR (CDCl3, 75 MHz): δ 177.5 (C-1), 27.3 (C-2), 45.9 (C-3), 68.1 (C-4), 20.9 (C-5), 65.5 (C-1′).


## 3. Result and Discussion

Ethyl acetate soluble fraction was subjected to repeated column chromatography on silica gel usingn-hexane and ethyl acetate as a solvent with gradual increasing in polarity up to 100% ethyl acetate, which resulted in four subfractions (FractionsA–D). The fractions obtained based on TLC profile were resubjected to pencil column chromatography and eluted withn-hexane : EtOAc, 25 : 75 andn-hexane: EtOAc, 30 : 70 to purify compound1(10.5 mg) and compound2 (9.8 mg) (Figure 1).Figure 1
Structure of compounds [1-2].Compound1 was isolated as a colorless oil and has molecular formula of C10H20O3 as suggested by molecular ion peak at m/z 188 M+ in HR-EIMS. The other fragment peaks were obtained at m/z 176, 157, 128, and 115. The HR-EIMS gave exact mass of compound1 which was at m/z 188.1420 (calcd. m/z 188.1412). The IR spectrum displayed absorption bands for hydroxyl and carbonyl groups at 3622 and 1714 cm−1, respectively. The 1H-NMR spectrum of compound1exhibited typical signal for aliphatic acid skeleton, which was strongly supported by DEPT experiment. The 13C-NMR spectrum revealed the presence of one methyl, one methine, seven methylene, and one quaternary carbon signals. The methine signal appearing at δH 3.62 (1H, m) was assigned to H-4 while methyl group resonated at δH 0.88 (3H, t,J = 7.7 Hz). Typical methylene signals were resonated as multiplet for seven methylene carbons at δH 2.08 (2H, m), δH 1.49 (1H, m), δH 1.45 (2H, m), δH 1.33 (1H, m), δH 1.47 (1H, m), δH 1.30 (2H, m), δH 1.28 (2H, m), and δH 1.29 (2H, m) assigned to H-2, H-3, H-5, H-6, H-7, H-8, and H-9, respectively (Table 1). The 13C-NMR spectrum (BB and DEPT) corroborated the presence of seven methylene carbons, one methine carbon, one terminal methyl carbon, and one quaternary carbon. The carbonyl carbon showed signals at δC 179.6 whereas methine signal centered at δC 72.1. The 13C-NMR chemical shift of CH3-C10 was observed at δC 13.9 and seven methylene carbons appeared at δC 34.6, 34.9, 37.9, 26.1, 29.8, 32.0, and 22.1 for C-2, C-3, C-5, C-6, C-7, C-8, and C-9, respectively (Table 2). The HMBC and COSY spectra were quite helpful for accurate placement of various substituents in the molecule. The HMBC spectrum showed strong correlation of methine proton at δH 3.62 (H-4) with C-2, C-3, C-5, and C-6 [13]. The methyl proton at δH 0.88 showed strong HMBC correlation with C-9 (δC 22.1), C-8 (δC 32.0) which was quite supportive in the establishment of structure. Finally all spectral data confirmed, compound1 as an aliphatic acid having straight chain of -(CH2)7-CH3- moiety [16] and was proposed to be 4-hydroxy decanoic acid.Table 1
1H-NMR (CDCl3, 300 MHz) data of compounds [1-2] in ppm, J in Hz.
Position
1
2
1
-
-
2
2.08, m
2.04, m2.35, m
3
1.62, m1.49, m
2.58, m
4
3.62, m
3.92, m
5
1.45, m
1.23 (1H, d,J = 6.9 Hz)
6
1.47, m1.33, m
-
7
1.30, m
-
8
1.28, m
-
9
1.29, m
-
10
0.88 (t,J= 7.7)
-
1′
-
4.31, mTable 2
13C-NMR (CDCl3, 75 MHz) of compounds [1-2] in ppm.
Position
1
2
1
179.6
177.5
2
34.6
27.3
3
34.9
45.9
4
72.1
68.1
5
37.9
20.9
6
26.1
-
7
29.8
-
8
32.0
-
9
22.1
-
10
13.9
-
1′
-
65.5Compound2was isolated as colorless oil. Its structure was mainly established by 1H-NMR and high resolution mass spectroscopy and supported by 13C-NMR spectrum. Its molecular formula C6H12O4 was concluded from the accurate mass measurement of peak at m/zM-OH+ 131, corresponding to molecular composition C6H11O3-OH. In addition to its molecular ion peak, it showed some characteristic fragments at m/z 115, 86, and 71. The HR-EIMS gave exact mass of compound2 at m/z 131.0744 (calcd. 131.0736 for C6H11O3-OH). The IR spectrum showed absorption bands at 3495 cm−1 and 1708 cm−1 indicating presence of the hydroxyl group and carbonyl carbon, respectively. Similarly, broad absorption centered at 2935 cm−1 suggested the presence of carboxylic acid. The 1H-NMR showed a signal for a secondary methyl group at δ 1.23 (3H, d,J = 6.9 Hz, H-5), connected to a methine group resonated at δH 3.92 (1H, m, H-4) possessing hydroxyl group, while the other methine signal appeared at δH 2.58 (1H, m, H-3). The downfield methylene bearing hydroxyl group appeared at δH 4.31 (2H, m, H-1′) and the other methylene centered at δH 2.04 (1H, m, H-2) and δH 2.35 (1H, m, H-2) was directly connected to carboxylic acid (Table 1). The 13C-NMR spectrum confirmed the presence of one methyl carbon, two methylene groups, two methine carbons, and one quaternary carbon in the structure.In13C-NMR spectrum, signal for secondary methyl appeared at δC 20.9 whereas the methine signal bearing hydroxyl group was observed at δC 68.1 for C-4. The signal for another methine appeared at δC 45.9 for C-3. The side chain methylene having free hydroxyl group resonated at δC 65.5, while the second methylene group at position C-2 appeared at δC 27.3. Similarly, the quaternary carbon in the form of carboxylic acid showed signal at δC 177.5 (Table 2). Based on the HMBC and H-H COSY correlation (Figure 2), the connectivity of the C-1 to C-5 chain was found in agreement with literature [17]. The HMBC spectrum showed H-C correlation of CH3-5 with that of C-4 and C-3. Similarly the position of hydroxyl group at C-1′ was confirmed by strong HMBC correlation of CH2-1′ with C-3, C-2, and C-4 and weak interaction with C-1. The structure of compound2 was mainly established by 1H-NMR, high resolution mass spectrometry and supported by 13C-NMR spectrum. From all spectral data it was evident that compound2was 4-hydroxy-3-(hydroxymethyl) pentanoic acid.Figure 2
HMBC correlation of compounds [1-2].


### 3.1. Antibacterial Activity

The antibacterial activity of isolated compounds [1-2] was determined by agar well diffusion method (Table 3). Compound1 was significantly active againstBacillus subtilis, Staphylococcus aureus, andMicrococcus luteus (Gram-positive). Both compounds [1-2] showed promising antibacterial activity againstStaphylococcus aureuswith 16 mm and 13 mm zone of inhibition. Compound2 was moderately active againstBacillus subtilisandMicrococcus luteuswith 5 mm and 9 mm zone of inhibition. Both compounds were inactive againstEscherichia coliandShigella flexneri.Table 3
Antibacterial activity of isolated compounds [1-2].
S. number
Culture
Zone of inhibition (mm)
1
2
Ciprofloxacin
1
Bacillus subtilis
8
5
8
2
Staphylococcus aureus
16
13
16
3
Micrococcus luteus
11
9
18
4
Pseudomonas pickettii
0
0
0
5
Escherichia coli
0
0
0
6
Shigella flexneri
6
9
14


### 3.2. Antifungal Activity

Antifungal activity of both compounds [1-2] was done againstAspergillus flavus andAspergillus niger. Both compounds [1-2] showed immense activity againstAspergillus niger with 15 mm ± 0.70 and 22 mm ± 0.57 zone of inhibition (Table 4). Moderate activity was observed by compound1 againstAspergillus flavushaving 12 mm ± 0.50 zone of inhibition.Table 4
Antifungal activity of isolated compounds [1-2].
Extract
Pathogenic fungi
Aspergillus flavus
Aspergillus niger
1
12 mm ± 0.50
15 mm ± 0.70
2
17 mm ± 0.28
22 mm ± 0.57
Nystatin (standard)
16 mm ± 0.92
21 mm ± 0.28
Note. Each value in the table was obtained by calculating the average of three experiments.


## 3.1. Antibacterial Activity

The antibacterial activity of isolated compounds [1-2] was determined by agar well diffusion method (Table 3). Compound1 was significantly active againstBacillus subtilis, Staphylococcus aureus, andMicrococcus luteus (Gram-positive). Both compounds [1-2] showed promising antibacterial activity againstStaphylococcus aureuswith 16 mm and 13 mm zone of inhibition. Compound2 was moderately active againstBacillus subtilisandMicrococcus luteuswith 5 mm and 9 mm zone of inhibition. Both compounds were inactive againstEscherichia coliandShigella flexneri.Table 3
Antibacterial activity of isolated compounds [1-2].
S. number
Culture
Zone of inhibition (mm)
1
2
Ciprofloxacin
1
Bacillus subtilis
8
5
8
2
Staphylococcus aureus
16
13
16
3
Micrococcus luteus
11
9
18
4
Pseudomonas pickettii
0
0
0
5
Escherichia coli
0
0
0
6
Shigella flexneri
6
9
14


## 3.2. Antifungal Activity

Antifungal activity of both compounds [1-2] was done againstAspergillus flavus andAspergillus niger. Both compounds [1-2] showed immense activity againstAspergillus niger with 15 mm ± 0.70 and 22 mm ± 0.57 zone of inhibition (Table 4). Moderate activity was observed by compound1 againstAspergillus flavushaving 12 mm ± 0.50 zone of inhibition.Table 4
Antifungal activity of isolated compounds [1-2].
Extract
Pathogenic fungi
Aspergillus flavus
Aspergillus niger
1
12 mm ± 0.50
15 mm ± 0.70
2
17 mm ± 0.28
22 mm ± 0.57
Nystatin (standard)
16 mm ± 0.92
21 mm ± 0.28
Note. Each value in the table was obtained by calculating the average of three experiments.


## 4. Conclusion

The current study describes the isolation, characterization, and antimicrobial activity of isolated compounds from ethyl acetate fraction ofQuercus incana. Both compounds displayed promising antimicrobial activity against human bacterial and fungal strains. Therefore, these isolated compounds may be considered as the lead compounds as an antimicrobial agents.



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*Source: 3798105-2018-01-28.xml*