# A Study on Herbal Finish to Prevent Bed Sore UsingMangifera indica and Triphala Dried Fruit

Authors: Kiruthika Deivasigamani; Siva Kumar Kolandaivel; Kavitha Krishnamoorthi
Journal: Journal of Textiles
 (2014)

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## Abstract

“Bed sores” owe their name to the observation that patients who were bedridden and not properly repositioned would often develop ulcerations or sores on their skin, typically over bony prominences. These bed sores, which result from prolonged pressure, are also called “decubitus ulcers,” “pressure sores,” “skin breakdown,” and “pressure ulcers.” They are associated with adverse health outcomes and high treatment costs. This study focuses on developing herbal finish to prevent bed sores. For this purpose, functional finishing was utilized to produce a bedsore preventing fabric. Plain weave cotton fabric was dyed by using herbal extracts, dried, and then cured. Two samples in different ratios (1 : 2 and 2 : 1) of two herbal extracts (Mangifera indica and Triphala dried fruits) were prepared with and without binder (chitosan). Air permeability, wicking ability, Drop Test for absorbency, and antibacterial properties were examined for those samples. Based on the results, fabric dyed with Mangifera indica and Triphala dried fruits in the ratio of 2 : 1 with chitosan is determined as the most promising combination for the final product.


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## Body

## 1. Introduction

The skin is a large barrier organ that protects the human body from environmental hazards (heat, cold, chemicals, mechanical forces, etc.) and maintains the integrity of the body, whereas the clothing system provides extra layer(s) of barrier to enhance the aesthetic, thermophysiological, and sensorial comfort of the wearer [1]. However, direct contact and interactions between textiles and skin may cause reactions, even damage or diseases [2] (see Figure 1).Figure 1
Time versus pressure curve for tissue breakdown in a canine model [2].Bed sores can result from one period of sustained pressure on the skin; however, most bed sores probably occur as a result of repeated incidents of blood flow interruption without adequate time for recovery. In fact, low pressure endured for long periods of time is believed to be more significant in producing pressure ulcers than higher pressure for shorter durations. The role that textiles play in the prevention and formation of these injuries is examined.


## 2. Materials and Methodology

### 2.1. Fabric Particulars

Plain weave cotton fabric with 60s yarn count, EPI: 88, and PPI: 86.


### 2.2. Herb Particulars

See Table1.Table 1
SI number
Botanical name
Common name
Chemical constituents
(1)
Mangifera indica [3]
Mango (leaf)
C-Glucoside xanthone mangiferin, tannins, alkaloids, glycosides, terpenoids, flavonoids, amino acids, proteins, euxanthin acid, and euxanthon.
(2)
Triphala dried fruits
(a)
Terminalia chebula [4]
Chebulic myrobalan
Tannins, chebulagic acid, chebulinic acid, ellagic acid, a tannin terchebin, an ellagitannin terchebulin, syringic acid, and gallic acid.
(b)
Terminalia bellerica [5]
Belleric myrobalan
Hexahydroxydiphenic acid ester,β-sitosterol, gallic acid, ellagic acid, ethyl gallate, galloyl glucose, chebulagic acid, mannitol, glucose, galactose, and rhamnose.
(c)
Emblica officinalis [6]
Indian gooseberry/amla
Organic acids—ascorbic acid, vitamin, tannin; bioflavonoids—quercetin, kaempferol; polyphenols—gallic acid; cytokinins—zeatin.


### 2.3. Methodology

The selected herbs were dried in sunshade and grinded. Methanol extract of the herbs was obtained and tested for antibacterial and wound healing property. Chitosan was then diluted with citric acid. Scoured and bleached fabric was padded, dried, and cured with and without binder. Fabric was then tested for its antibacterial and wound healing property, washing fastness, air permeability, wicking ability, and water absorbency. Finally its results were discussed (see Figure2).Figure 2
Herbal extract along with binder.


### 2.4. Finishing Agents

Methanol extract ofMangifera indica and Triphala dried fruits (Terminalia chebula,Terminalia bellerica, andEmblica officinalis) has the antibacterial and wound healing property. 25 grams of herbal powder is dissolved in 100 mL of methanol and then it is concentrated into 50 mL using Soxhlet apparatus to obtain the extract.


#### 2.4.1. Recipe

See Table2.Table 2
Samples
M1
M2
C1
C2
Mangifera indica extract
2%
1%
2%
1%
Triphala dried fruits extract
1%
2%
1%
2%
Chitosan diluted with citric acid (fabric dipped for 30 min)
*
*
6%
6%
MLR ratio (fabric dipped for 2 hrs)
1 : 20
1 : 20
1 : 20
1 : 20
Drying (for 5 min)
80°C
80°C
80°C
80°C
Curing (for 2 min)
150–180°C
150–180°C
150–180°C
150–180°C
* denotes that samples M1 and M2 are not treated with Chitosan.


## 2.1. Fabric Particulars

Plain weave cotton fabric with 60s yarn count, EPI: 88, and PPI: 86.


## 2.2. Herb Particulars

See Table1.Table 1
SI number
Botanical name
Common name
Chemical constituents
(1)
Mangifera indica [3]
Mango (leaf)
C-Glucoside xanthone mangiferin, tannins, alkaloids, glycosides, terpenoids, flavonoids, amino acids, proteins, euxanthin acid, and euxanthon.
(2)
Triphala dried fruits
(a)
Terminalia chebula [4]
Chebulic myrobalan
Tannins, chebulagic acid, chebulinic acid, ellagic acid, a tannin terchebin, an ellagitannin terchebulin, syringic acid, and gallic acid.
(b)
Terminalia bellerica [5]
Belleric myrobalan
Hexahydroxydiphenic acid ester,β-sitosterol, gallic acid, ellagic acid, ethyl gallate, galloyl glucose, chebulagic acid, mannitol, glucose, galactose, and rhamnose.
(c)
Emblica officinalis [6]
Indian gooseberry/amla
Organic acids—ascorbic acid, vitamin, tannin; bioflavonoids—quercetin, kaempferol; polyphenols—gallic acid; cytokinins—zeatin.


## 2.3. Methodology

The selected herbs were dried in sunshade and grinded. Methanol extract of the herbs was obtained and tested for antibacterial and wound healing property. Chitosan was then diluted with citric acid. Scoured and bleached fabric was padded, dried, and cured with and without binder. Fabric was then tested for its antibacterial and wound healing property, washing fastness, air permeability, wicking ability, and water absorbency. Finally its results were discussed (see Figure2).Figure 2
Herbal extract along with binder.


## 2.4. Finishing Agents

Methanol extract ofMangifera indica and Triphala dried fruits (Terminalia chebula,Terminalia bellerica, andEmblica officinalis) has the antibacterial and wound healing property. 25 grams of herbal powder is dissolved in 100 mL of methanol and then it is concentrated into 50 mL using Soxhlet apparatus to obtain the extract.


### 2.4.1. Recipe

See Table2.Table 2
Samples
M1
M2
C1
C2
Mangifera indica extract
2%
1%
2%
1%
Triphala dried fruits extract
1%
2%
1%
2%
Chitosan diluted with citric acid (fabric dipped for 30 min)
*
*
6%
6%
MLR ratio (fabric dipped for 2 hrs)
1 : 20
1 : 20
1 : 20
1 : 20
Drying (for 5 min)
80°C
80°C
80°C
80°C
Curing (for 2 min)
150–180°C
150–180°C
150–180°C
150–180°C
* denotes that samples M1 and M2 are not treated with Chitosan.


## 2.4.1. Recipe

See Table2.Table 2
Samples
M1
M2
C1
C2
Mangifera indica extract
2%
1%
2%
1%
Triphala dried fruits extract
1%
2%
1%
2%
Chitosan diluted with citric acid (fabric dipped for 30 min)
*
*
6%
6%
MLR ratio (fabric dipped for 2 hrs)
1 : 20
1 : 20
1 : 20
1 : 20
Drying (for 5 min)
80°C
80°C
80°C
80°C
Curing (for 2 min)
150–180°C
150–180°C
150–180°C
150–180°C
* denotes that samples M1 and M2 are not treated with Chitosan.


## 3. Results and Discussion

### 3.1. Wickability

Fabric specimen is cut into the size of12′′×1′′ and hung over the stand. A beaker is filled with tinted distilled water. The lower end of the fabric strip is made to touch the water in such a way that half an inch height of the fabric is kept immersed inside the water. To keep the fabric strip in straight configuration a load of 10 g of weight is applied to the bottom of it. The rise of the water level in the fabric is measured after 10 minutes.The wickability of treated and untreated plain weave cotton fabric samples was assessed by time for wetting. The vertical wicking test results are shown the table.


#### 3.1.1. Wickability Test Results

The wicking property of the finished fabric has been improved for all the samples when compared with the bleached fabric. Wickability of the samples treated with chitosan is highly increased (see Table3 and Figure 3).Table 3
Wickability result of the treated and untreated fabric samples.
SI number
Type of the sample
Wicking height in centimeters
1
Bleached fabric
5
2
Sample 1-M1
7.5
3
Sample 2-M2
7.5
4
Sample 3-C1
9
5
Sample 4-C2
9.5Figure 3
Comparison of wickability results of the treated and untreated samples.


### 3.2. Drop Test

It is used to measure the time taken for the fabric to absorb a drop of water. The absorption rate of fabric changes as per the thickness of the fabric, weave structure, and finishes applied to the fabric.


#### 3.2.1. Drop Test Results

The time taken to absorb a drop of water is decreased in the finished samples when compared with the bleached sample. This shows that the absorbency is increased after finishing. Comparing to all the four samples sample 3-C1 showed good absorbency (see Table4 and Figure 4).Table 4
Drop Test result for the treated and untreated fabric samples.
SI number
Name of the samples
Time taken to absorb a drop of water in seconds
Trial 1
Trial 2
Trial 3
AVG
1
Bleached fabric
11.36
10.91
10.84
11.03
2
Sample 1-M1
11.25
11.29
12.50
11.68
3
Sample 2-M2
12.62
12.89
12.08
12.53
4
Sample 3-C1
9.56
9.03
9.33
9.30
5
Sample 4-C2
10.36
10.37
10.67
10.46Figure 4
Comparison of Drop Test results of the treated and untreated fabric samples.


### 3.3. Air Permeability

It is the volume of air measured in cubic cm passed per second through 1 cm2 for the fabric at a pressure one cm head of water. It is measured by using air permeability tester. The air permeability of a fabric is a measure of how well it allows the passage of air through it.


#### 3.3.1. Air Permeability Test to Various Fabrics

Air permeability is an important factor in the performance of textile materials used to provide an indication of the breathability of coated fabrics. The air permeability of treated and untreated plain weave of cotton fabric samples was assessed. Consider(1)Airpermeability=1000*r3600*A,
where r = rotameter reading and A=4 cm2 (testing area).


#### 3.3.2. Air Permeability Test Results

Air permeability of the plain weave cotton fabric is high in all the treated samples compared to the untreated samples (see Table5 and Figure 5).Table 5
Average value evaluation of air permeability.
SI number
Name of the samples
Rotameter reading
Air permeability
Air permeability
Trial 1
Trial 2
AVG
(cc/sec/cm)2
(cc/sec/cm)2/60
1
Bleached fabric
242
304
273
18.9
0.315
2
Sample 1-M1
376
344
360
25
0.416
3
Sample 2-M2
450
410
430
29.8
0.496
4
Sample 3-C1
360
370
365
25.3
0.422
5
Sample 4-C2
480
490
485
33.6
0.561Figure 5
Average value evaluation of air permeability.


### 3.4. Antibacterial Test

#### 3.4.1. Results of Antibacterial Activity of the Herbal Extracts (Kirby-Bauer Disk Diffusion Susceptibility Test)

Mangifera indica and Triphala dried fruits have antibacterial activity againstStaphylococcus aureus (see Table 6 and Figure 6).Table 6
Antibacterial activity of the herbal extract.
Plant extract
Staphylococcus aureus
Pseudomonas aeruginosa
Concentration
25μL
50μL
75μL
100μL
25μL
50μL
75μL
100μL
Mangifera indica
Nil
Nil
6 mm
11 mm
Nil
Nil
8 mm
12 mm
Triphala dried fruits
11 mm
13 mm
15 mm
21 mm
10 mm
12 mm
14 mm
16 mmFigure 6
Antibacterial activity of the herbal extract.


#### 3.4.2. Results of Antibacterial Activity of Textile Fabric

Only sample 1-M1 and sample 3-C1 have antibacterial activity againstStaphylococcus aureus (see Table 7 and Figure 7).Table 7
Antibacterial activity of textile fabric coated with the herbal extract.
SI number
Name of the samples
Staphylococcus aureus
Pseudomonas aeruginosa
1
Sample 1-M1
3 mm
Nil
2
Sample 2-M2
Nil
Nil
3
Sample 3-C1
11 mm
Nil
4
Sample 4-C2
Nil
Nil
Control (chloramphenicol)
24 mm
10 mmFigure 7
Antibacterial test of the treated samples.


### 3.5. Wound Healing Test: Angiogenic Activity of the Herbal Extract and Herbal Based Textile Fabric

The herbal extract of Triphala dried fruits containing compounds with angiogenesis modulating properties showed strong angiogenic activity in CAM (chorioallantoic membrane) treated with herbal extract, by increasing the size and number of blood vessels as compared to the control.The herbal based treated cloth C1 showed better angiogenic activity in CAM. The size and number of blood vessels were high compared to control egg treated with saline (see Table8 and Figure 8).Table 8
Angiogenic activity of herbal extract and herbal based textile fabric.
Sample name
Number of blood vessel formation
Triphala dried fruits
12
M1
Nil
M2
Nil
C1
16
C2
Nil
Control
8Figure 8
Wound healing test of the treated sample C1.


### 3.6. Washing Fastness Test

#### 3.6.1. Results of Washing Fastness Test for Its Antibacterial Property

Only sample C1 has the washing fastness of antibacterial property for up to 3 washes, which shows that chitosan acts as a binder up to certain level (see Table9).Table 9
Antibacterial activity of fabric coated with the herbal extract (for washing fastness).
SI number
Name of the sample
Staphylococcus aureus
1
C1, up to 3 washes
3 mm
2
C1, up to 5 washes
Nil
Control (chloramphenicol)
24 mm


#### 3.6.2. Results of Washing Fastness Test for Its Wound Healing Property

Only sample C1 has the washing fastness for wound healing property for more than 10 washes, which shows that chitosan acts as a binder up to certain level (see Table10).Table 10
Wound healing activity of fabric coated with the herbal extract (for washing fastness).
Sample name
Number of blood vessel formation
C1, up to 3 washes
14
C1, up to 5 washes
13
C1, up to 10 washes
10
Control
8


## 3.1. Wickability

Fabric specimen is cut into the size of12′′×1′′ and hung over the stand. A beaker is filled with tinted distilled water. The lower end of the fabric strip is made to touch the water in such a way that half an inch height of the fabric is kept immersed inside the water. To keep the fabric strip in straight configuration a load of 10 g of weight is applied to the bottom of it. The rise of the water level in the fabric is measured after 10 minutes.The wickability of treated and untreated plain weave cotton fabric samples was assessed by time for wetting. The vertical wicking test results are shown the table.


### 3.1.1. Wickability Test Results

The wicking property of the finished fabric has been improved for all the samples when compared with the bleached fabric. Wickability of the samples treated with chitosan is highly increased (see Table3 and Figure 3).Table 3
Wickability result of the treated and untreated fabric samples.
SI number
Type of the sample
Wicking height in centimeters
1
Bleached fabric
5
2
Sample 1-M1
7.5
3
Sample 2-M2
7.5
4
Sample 3-C1
9
5
Sample 4-C2
9.5Figure 3
Comparison of wickability results of the treated and untreated samples.


## 3.1.1. Wickability Test Results

The wicking property of the finished fabric has been improved for all the samples when compared with the bleached fabric. Wickability of the samples treated with chitosan is highly increased (see Table3 and Figure 3).Table 3
Wickability result of the treated and untreated fabric samples.
SI number
Type of the sample
Wicking height in centimeters
1
Bleached fabric
5
2
Sample 1-M1
7.5
3
Sample 2-M2
7.5
4
Sample 3-C1
9
5
Sample 4-C2
9.5Figure 3
Comparison of wickability results of the treated and untreated samples.


## 3.2. Drop Test

It is used to measure the time taken for the fabric to absorb a drop of water. The absorption rate of fabric changes as per the thickness of the fabric, weave structure, and finishes applied to the fabric.


### 3.2.1. Drop Test Results

The time taken to absorb a drop of water is decreased in the finished samples when compared with the bleached sample. This shows that the absorbency is increased after finishing. Comparing to all the four samples sample 3-C1 showed good absorbency (see Table4 and Figure 4).Table 4
Drop Test result for the treated and untreated fabric samples.
SI number
Name of the samples
Time taken to absorb a drop of water in seconds
Trial 1
Trial 2
Trial 3
AVG
1
Bleached fabric
11.36
10.91
10.84
11.03
2
Sample 1-M1
11.25
11.29
12.50
11.68
3
Sample 2-M2
12.62
12.89
12.08
12.53
4
Sample 3-C1
9.56
9.03
9.33
9.30
5
Sample 4-C2
10.36
10.37
10.67
10.46Figure 4
Comparison of Drop Test results of the treated and untreated fabric samples.


## 3.2.1. Drop Test Results

The time taken to absorb a drop of water is decreased in the finished samples when compared with the bleached sample. This shows that the absorbency is increased after finishing. Comparing to all the four samples sample 3-C1 showed good absorbency (see Table4 and Figure 4).Table 4
Drop Test result for the treated and untreated fabric samples.
SI number
Name of the samples
Time taken to absorb a drop of water in seconds
Trial 1
Trial 2
Trial 3
AVG
1
Bleached fabric
11.36
10.91
10.84
11.03
2
Sample 1-M1
11.25
11.29
12.50
11.68
3
Sample 2-M2
12.62
12.89
12.08
12.53
4
Sample 3-C1
9.56
9.03
9.33
9.30
5
Sample 4-C2
10.36
10.37
10.67
10.46Figure 4
Comparison of Drop Test results of the treated and untreated fabric samples.


## 3.3. Air Permeability

It is the volume of air measured in cubic cm passed per second through 1 cm2 for the fabric at a pressure one cm head of water. It is measured by using air permeability tester. The air permeability of a fabric is a measure of how well it allows the passage of air through it.


### 3.3.1. Air Permeability Test to Various Fabrics

Air permeability is an important factor in the performance of textile materials used to provide an indication of the breathability of coated fabrics. The air permeability of treated and untreated plain weave of cotton fabric samples was assessed. Consider(1)Airpermeability=1000*r3600*A,
where r = rotameter reading and A=4 cm2 (testing area).


### 3.3.2. Air Permeability Test Results

Air permeability of the plain weave cotton fabric is high in all the treated samples compared to the untreated samples (see Table5 and Figure 5).Table 5
Average value evaluation of air permeability.
SI number
Name of the samples
Rotameter reading
Air permeability
Air permeability
Trial 1
Trial 2
AVG
(cc/sec/cm)2
(cc/sec/cm)2/60
1
Bleached fabric
242
304
273
18.9
0.315
2
Sample 1-M1
376
344
360
25
0.416
3
Sample 2-M2
450
410
430
29.8
0.496
4
Sample 3-C1
360
370
365
25.3
0.422
5
Sample 4-C2
480
490
485
33.6
0.561Figure 5
Average value evaluation of air permeability.


## 3.3.1. Air Permeability Test to Various Fabrics

Air permeability is an important factor in the performance of textile materials used to provide an indication of the breathability of coated fabrics. The air permeability of treated and untreated plain weave of cotton fabric samples was assessed. Consider(1)Airpermeability=1000*r3600*A,
where r = rotameter reading and A=4 cm2 (testing area).


## 3.3.2. Air Permeability Test Results

Air permeability of the plain weave cotton fabric is high in all the treated samples compared to the untreated samples (see Table5 and Figure 5).Table 5
Average value evaluation of air permeability.
SI number
Name of the samples
Rotameter reading
Air permeability
Air permeability
Trial 1
Trial 2
AVG
(cc/sec/cm)2
(cc/sec/cm)2/60
1
Bleached fabric
242
304
273
18.9
0.315
2
Sample 1-M1
376
344
360
25
0.416
3
Sample 2-M2
450
410
430
29.8
0.496
4
Sample 3-C1
360
370
365
25.3
0.422
5
Sample 4-C2
480
490
485
33.6
0.561Figure 5
Average value evaluation of air permeability.


## 3.4. Antibacterial Test

### 3.4.1. Results of Antibacterial Activity of the Herbal Extracts (Kirby-Bauer Disk Diffusion Susceptibility Test)

Mangifera indica and Triphala dried fruits have antibacterial activity againstStaphylococcus aureus (see Table 6 and Figure 6).Table 6
Antibacterial activity of the herbal extract.
Plant extract
Staphylococcus aureus
Pseudomonas aeruginosa
Concentration
25μL
50μL
75μL
100μL
25μL
50μL
75μL
100μL
Mangifera indica
Nil
Nil
6 mm
11 mm
Nil
Nil
8 mm
12 mm
Triphala dried fruits
11 mm
13 mm
15 mm
21 mm
10 mm
12 mm
14 mm
16 mmFigure 6
Antibacterial activity of the herbal extract.


### 3.4.2. Results of Antibacterial Activity of Textile Fabric

Only sample 1-M1 and sample 3-C1 have antibacterial activity againstStaphylococcus aureus (see Table 7 and Figure 7).Table 7
Antibacterial activity of textile fabric coated with the herbal extract.
SI number
Name of the samples
Staphylococcus aureus
Pseudomonas aeruginosa
1
Sample 1-M1
3 mm
Nil
2
Sample 2-M2
Nil
Nil
3
Sample 3-C1
11 mm
Nil
4
Sample 4-C2
Nil
Nil
Control (chloramphenicol)
24 mm
10 mmFigure 7
Antibacterial test of the treated samples.


## 3.4.1. Results of Antibacterial Activity of the Herbal Extracts (Kirby-Bauer Disk Diffusion Susceptibility Test)

Mangifera indica and Triphala dried fruits have antibacterial activity againstStaphylococcus aureus (see Table 6 and Figure 6).Table 6
Antibacterial activity of the herbal extract.
Plant extract
Staphylococcus aureus
Pseudomonas aeruginosa
Concentration
25μL
50μL
75μL
100μL
25μL
50μL
75μL
100μL
Mangifera indica
Nil
Nil
6 mm
11 mm
Nil
Nil
8 mm
12 mm
Triphala dried fruits
11 mm
13 mm
15 mm
21 mm
10 mm
12 mm
14 mm
16 mmFigure 6
Antibacterial activity of the herbal extract.


## 3.4.2. Results of Antibacterial Activity of Textile Fabric

Only sample 1-M1 and sample 3-C1 have antibacterial activity againstStaphylococcus aureus (see Table 7 and Figure 7).Table 7
Antibacterial activity of textile fabric coated with the herbal extract.
SI number
Name of the samples
Staphylococcus aureus
Pseudomonas aeruginosa
1
Sample 1-M1
3 mm
Nil
2
Sample 2-M2
Nil
Nil
3
Sample 3-C1
11 mm
Nil
4
Sample 4-C2
Nil
Nil
Control (chloramphenicol)
24 mm
10 mmFigure 7
Antibacterial test of the treated samples.


## 3.5. Wound Healing Test: Angiogenic Activity of the Herbal Extract and Herbal Based Textile Fabric

The herbal extract of Triphala dried fruits containing compounds with angiogenesis modulating properties showed strong angiogenic activity in CAM (chorioallantoic membrane) treated with herbal extract, by increasing the size and number of blood vessels as compared to the control.The herbal based treated cloth C1 showed better angiogenic activity in CAM. The size and number of blood vessels were high compared to control egg treated with saline (see Table8 and Figure 8).Table 8
Angiogenic activity of herbal extract and herbal based textile fabric.
Sample name
Number of blood vessel formation
Triphala dried fruits
12
M1
Nil
M2
Nil
C1
16
C2
Nil
Control
8Figure 8
Wound healing test of the treated sample C1.


## 3.6. Washing Fastness Test

### 3.6.1. Results of Washing Fastness Test for Its Antibacterial Property

Only sample C1 has the washing fastness of antibacterial property for up to 3 washes, which shows that chitosan acts as a binder up to certain level (see Table9).Table 9
Antibacterial activity of fabric coated with the herbal extract (for washing fastness).
SI number
Name of the sample
Staphylococcus aureus
1
C1, up to 3 washes
3 mm
2
C1, up to 5 washes
Nil
Control (chloramphenicol)
24 mm


### 3.6.2. Results of Washing Fastness Test for Its Wound Healing Property

Only sample C1 has the washing fastness for wound healing property for more than 10 washes, which shows that chitosan acts as a binder up to certain level (see Table10).Table 10
Wound healing activity of fabric coated with the herbal extract (for washing fastness).
Sample name
Number of blood vessel formation
C1, up to 3 washes
14
C1, up to 5 washes
13
C1, up to 10 washes
10
Control
8


## 3.6.1. Results of Washing Fastness Test for Its Antibacterial Property

Only sample C1 has the washing fastness of antibacterial property for up to 3 washes, which shows that chitosan acts as a binder up to certain level (see Table9).Table 9
Antibacterial activity of fabric coated with the herbal extract (for washing fastness).
SI number
Name of the sample
Staphylococcus aureus
1
C1, up to 3 washes
3 mm
2
C1, up to 5 washes
Nil
Control (chloramphenicol)
24 mm


## 3.6.2. Results of Washing Fastness Test for Its Wound Healing Property

Only sample C1 has the washing fastness for wound healing property for more than 10 washes, which shows that chitosan acts as a binder up to certain level (see Table10).Table 10
Wound healing activity of fabric coated with the herbal extract (for washing fastness).
Sample name
Number of blood vessel formation
C1, up to 3 washes
14
C1, up to 5 washes
13
C1, up to 10 washes
10
Control
8


## 4. Conclusion

In this work, herbal based antibacterial finish was applied to the cotton fabric. Extracts ofMangifera indica and Triphala dried fruit were applied to the fabric in two different ratios with and without the binder chitosan.Based on the tests conducted, the obtained results were shown in the tables and figures. According to the results, the treated samples M1 and C1 showed good antibacterial property and sample C1 showed excellent wound healing property. The samples treated with chitosan have high wickability and water absorbency properties. Air permeability in the bleached fabric is low and it has been increased in all the treated samples. Washing fastness result shows that the fabric treated with chitosan shows antibacterial property for up to 3 washes and wound healing property for more than 10 washes.So it is concluded that sample C1 has much better properties for preventing bed sore. The herbal extract ofMangifera indica shows good antibacterial property when applied to the fabric compared to the Triphala dried fruits and chitosan has good binding property. And it is suggested that this fabric could be used for preventing bed sore and also for other applications where antibacterial finish is required. And also it gives cost-wise benefit to people. In addition, it is environment friendly.



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*Source: 368929-2014-12-01.xml*