# Effects of Electrode Reversal on the Distribution of Naproxen in the Electrospun Cellulose Acetate Nanofibers

Authors: Zhuang Li; Hongliang Kang; Ning Che; Zhijing Liu; Pingping Li; Weiwei Li; Chao Zhang; Chun Cao; Ruigang Liu; Yong Huang
Journal: Journal of Nanomaterials
 (2014)

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## Abstract

Naproxen (NAP)/cellulose acetate hybrid nanofibers were prepared by positive and reversed emitting electrodes electrospinning setups. The morphology and structure of the resultant nanofibers were characterized, and the NAP release behaviors were investigated. It was found that NAP dispersed in the CA matrix in molecular level, and no aggregation and dimers of NAP were found in the resultant NAP/CA hybrid nanofibers due to the formation of hydrogen bonds between NAP and CA. The nanofibers obtained by reversed emitting electrode electrospinning setup have a thicker diameter and a faster NAP release rate compared with those obtained by positive emitting electrode electrospinning setup. The faster drug release of NAP from nanofibers prepared by reversed emitting electrode electrospinning is due to the fact that the concentration of NAP molecules near the surface of the nanofibers is relatively higher than that of the nanofibers prepared by positive emitting electrode electrospinning setup. The effects of the electrode polarity on the distribution of drugs in nanofibers can be used to prepare hybrid electrospun fibers of different drug release rates, which may found applications in biomedical materials.


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## Body

## 1. Introduction

Electrospinning is a cost effective and straightforward technique to produce nanofibers with diameters in the range of 50 nm to 5μm [1, 2]. The resultant nanofiber mats are attractive materials for their superior specific surface area and high porosity, which have promising applications in filter [3–6], catalyst [7–9], sensor [10–12], and biomedical materials [13–15]. In the process of electrospinning, a continuous charged jet is initiated when the electric field force exerted on the pendent drop exceeds its own surface tension. The flying jet then undergoes straight movement and whipping motion and finally solidifies on the collector as a nonwoven mat [16, 17]. Due to the complexity of electrohydrodynamics, the process of electrospinning was affected by various factors including solution properties, experimental parameters, and environmental conditions [18, 19]. Among various process parameters, volumetric charge density, distance between nozzle and collector, initial jet radius, relaxation time, and solution viscosity have the most significant effects on the flying jet [20]. The polarity of the emitting electrode, say the one that is in contact with the polymer solution or melt, can be either positive or negative in electrospinning. Generally, the emitting electrode is positive. Meanwhile, electrospinning with negative emitting electrode has also been applied for preparing nanofibers, and the effects of the electric field morphology on the resultant nanofibers have been investigated [21–23]. It was found that the prepared nanofibers usually have larger diameter and pore size in the case of using negative emitting electrode, which is attributed to the relatively small Coulombic repulsion force on the flying jets [24].Due to the similarity to extracellular matrix (ECM), electrospun nanofibers loading various drugs and biomolecules have been used as wound dressing, surgical scaffold, and orthopedic materials [25–27]. The conventional method of producing these composite nanofibers was directly electrospinning the mixed solution of polymers and contents. It is reported that the loaded drugs were embedded in the polymer fibers and diffused out with a burst release in in vitro dissolution test, which is due to the interaction between the drugs and the polymer molecules [28, 29]. Naproxen (NAP) as an effective nonsteroidal anti-inflammatory drug is commonly used to treat the inflammation and pain of various wounds [30]. Owing to gastrointestinal irritancy caused by oral administration, NAP is more appropriate for topical application [31].In this work, cellulose acetated (CA) nanofibers loaded with naproxen were prepared by electrospinning with positive and reversed emitting electrodes setups. CA and NAP mixed solution was used in the electrospinning. The morphology of the resultant nanofibers and the drug distribution were investigated and discussed.


## 2. Materials and Methods

### 2.1. Materials

Cellulose acetate (CA,Mr=29 kDa) was purchased from Sigma Aldrich (USA). Naproxen (NAP) was purchased from Alfa Aesar (USA). Highly purified water was prepared using Milli-Q Water Purification System (Millipore, USA). Dimethyl sulfoxide (DMSO) was supplied by Beijing Chemical Works and distilled before use. Other chemicals including acetone andN, N-dimethylacetamide (DMAc) are all of analytical grade and were used as received.


### 2.2. Preparation of NAP-Loaded Nanofibers

19% (w/v) CA solution was prepared by dissolving CA in a mixed solution of acetone and DMAc (2 : 1, v/v). Then, 10 wt% NAP (based on the weight of CA) was added into the base CA solution to get the mixed solution for electrospinning. The conductivity of the CA solution and the NAP/CA mixed solution was measured using a conductivity meter (Rex DDS-307A, INESA, China).Two setups with inverse emitting electrode polarity, say positive and reversed emitting electrodes, were used for electrospinning (Figure1). The other parameters were the same for the two setups. The inner diameter of the needle was 1.0 mm and the extruding rate was 0.25 mL/h. The applied voltage was 15 kV and the distance between the needle and the collector plate was 12 cm. All the electrospinning experiments were carried out at room temperature with a humidity of about 20–30%. The electric current of both electrospinning setups was determined using a multimeter (Fluke 15B, USA).Schematic illustration of the electrospinning setup with (a) positive and (b) reversed emitting electrodes.
(a)
(b)


### 2.3. Instruments and Characterization

The morphology of the prepared fibers was observed on a field emission scanning electron microscopy (FESEM, JEOL 6700, Japan) operated at an accelerating voltage of 5 kV and current of 10μA. The samples were sputter-coated with a layer of platinum using a sputter-coater (Bal-Tec, SCD 500, Switzerland) before observation. All the images were taken in the secondary electron mode. Elemental analysis was performed on an energy dispersive X-ray spectrometer (EDS, EDAX-TSL, USA). Transmission electron microscopy (TEM) observation of the nanofibers was carried out on a field emission transmission electron microscopy (FETEM, 2200FS, JEOL, Japan). The samples were prepared by fixing copper grids on the collector and collecting fibers during the process of electrospinning. The operation voltage of TEM was set to 200 kV, and the images were recorded in the bright field mode.Differential scanning calorimetry (DSC) experiments were carried out on DSC Q2000 (TA Instruments, USA) in the range of 20–200°C at the heating rate of 10°C/min in nitrogen atmosphere.Wide angle X-ray diffraction (WAXD) experiments were carried out on a D/max 2500 X-ray diffraction meter (Rigaku, Japan). CuKα X-ray (λ= 0.154 nm) was used as the radiation source. Operation parameters were set at 40 kV and 200 mA and the data in the range of 2θ = 5–60° were recorded.Fourier transform infrared spectroscopy (FTIR, EQUINOX55, Bruker, Germany) was used to characterize the fiber mats at a range of 400 cm−1 to 4000 cm−1 and a resolution of 2 cm−1.UV-Vis spectrophotometer (TU-1901, Purkinje General, China) was used to determine the NAP concentration in the buffer solutions. The wavelength of the emitting UV-Vis light was selected as 262 nm.


### 2.4. In Vitro Drug Release

The in vitro drug release experiments of both NAP/CA nanofibers were carried out as follows. A certain amount of nanofibers were placed in a dialysis bag with molecular weight cutoff of 3.50 kDa. The dialysis bag was then immersed in a phosphate buffer solution with a desired volume. The temperature of the phosphate buffer solution (pH= 6.4) was set at 37±0.5°C to mimic the physiological conditions of human skin. The out medium was stirred continuously at 200 rpm and the NAP concentration in the out buffer solution was determined at desired time by UV-Vis absorbance.The accumulative release of NAP from the nanofibers,P(%), was calculated using the following equation [32]:
(1)P(%)=Cn×V0+∑i=1n-1Ci×VW,
where Cn is the NAP concentration determined at number n and Ci is the NAP concentration determined at number i; V0 is the volume of the buffer and V is the volume of the withdrawn sample; and W is the total amount of NAP in the fibers. Three parallel experiments were carried out and the mean value was plotted as a function of time.


## 2.1. Materials

Cellulose acetate (CA,Mr=29 kDa) was purchased from Sigma Aldrich (USA). Naproxen (NAP) was purchased from Alfa Aesar (USA). Highly purified water was prepared using Milli-Q Water Purification System (Millipore, USA). Dimethyl sulfoxide (DMSO) was supplied by Beijing Chemical Works and distilled before use. Other chemicals including acetone andN, N-dimethylacetamide (DMAc) are all of analytical grade and were used as received.


## 2.2. Preparation of NAP-Loaded Nanofibers

19% (w/v) CA solution was prepared by dissolving CA in a mixed solution of acetone and DMAc (2 : 1, v/v). Then, 10 wt% NAP (based on the weight of CA) was added into the base CA solution to get the mixed solution for electrospinning. The conductivity of the CA solution and the NAP/CA mixed solution was measured using a conductivity meter (Rex DDS-307A, INESA, China).Two setups with inverse emitting electrode polarity, say positive and reversed emitting electrodes, were used for electrospinning (Figure1). The other parameters were the same for the two setups. The inner diameter of the needle was 1.0 mm and the extruding rate was 0.25 mL/h. The applied voltage was 15 kV and the distance between the needle and the collector plate was 12 cm. All the electrospinning experiments were carried out at room temperature with a humidity of about 20–30%. The electric current of both electrospinning setups was determined using a multimeter (Fluke 15B, USA).Schematic illustration of the electrospinning setup with (a) positive and (b) reversed emitting electrodes.
(a)
(b)


## 2.3. Instruments and Characterization

The morphology of the prepared fibers was observed on a field emission scanning electron microscopy (FESEM, JEOL 6700, Japan) operated at an accelerating voltage of 5 kV and current of 10μA. The samples were sputter-coated with a layer of platinum using a sputter-coater (Bal-Tec, SCD 500, Switzerland) before observation. All the images were taken in the secondary electron mode. Elemental analysis was performed on an energy dispersive X-ray spectrometer (EDS, EDAX-TSL, USA). Transmission electron microscopy (TEM) observation of the nanofibers was carried out on a field emission transmission electron microscopy (FETEM, 2200FS, JEOL, Japan). The samples were prepared by fixing copper grids on the collector and collecting fibers during the process of electrospinning. The operation voltage of TEM was set to 200 kV, and the images were recorded in the bright field mode.Differential scanning calorimetry (DSC) experiments were carried out on DSC Q2000 (TA Instruments, USA) in the range of 20–200°C at the heating rate of 10°C/min in nitrogen atmosphere.Wide angle X-ray diffraction (WAXD) experiments were carried out on a D/max 2500 X-ray diffraction meter (Rigaku, Japan). CuKα X-ray (λ= 0.154 nm) was used as the radiation source. Operation parameters were set at 40 kV and 200 mA and the data in the range of 2θ = 5–60° were recorded.Fourier transform infrared spectroscopy (FTIR, EQUINOX55, Bruker, Germany) was used to characterize the fiber mats at a range of 400 cm−1 to 4000 cm−1 and a resolution of 2 cm−1.UV-Vis spectrophotometer (TU-1901, Purkinje General, China) was used to determine the NAP concentration in the buffer solutions. The wavelength of the emitting UV-Vis light was selected as 262 nm.


## 2.4. In Vitro Drug Release

The in vitro drug release experiments of both NAP/CA nanofibers were carried out as follows. A certain amount of nanofibers were placed in a dialysis bag with molecular weight cutoff of 3.50 kDa. The dialysis bag was then immersed in a phosphate buffer solution with a desired volume. The temperature of the phosphate buffer solution (pH= 6.4) was set at 37±0.5°C to mimic the physiological conditions of human skin. The out medium was stirred continuously at 200 rpm and the NAP concentration in the out buffer solution was determined at desired time by UV-Vis absorbance.The accumulative release of NAP from the nanofibers,P(%), was calculated using the following equation [32]:
(1)P(%)=Cn×V0+∑i=1n-1Ci×VW,
where Cn is the NAP concentration determined at number n and Ci is the NAP concentration determined at number i; V0 is the volume of the buffer and V is the volume of the withdrawn sample; and W is the total amount of NAP in the fibers. Three parallel experiments were carried out and the mean value was plotted as a function of time.


## 3. Results and Discussion

### 3.1. Preparation of NAP/CA Nanofibers

NAP/CA hybrid nanofiber mats can be successfully prepared by both positive and reversed emitting electrode setups (Figure S1); see Figure S1 in Supplementary Material available online athttp://dx.doi.org/10.1155/2014/360658. Figure 2 shows the typical SEM images of the NAP/CA nanofibers prepared by electrospinning using positive and reversed emitting electrodes. The results indicate that the resultant nanofibers have similar morphology. The nanofibers have smooth surfaces and no particles can be observed, suggesting no NAP aggregations formed outside of the hybrid nanofibers. Statistics of the diameter of the nanofibers indicates that the nanofibers prepared by reversed emitting electrode electrospinning have a larger average diameter and a narrower diameter distribution (594.6±197.2 nm) compared with those nanofibers prepared by using positive emitting electrode setup (574.4±212.2 nm) (Figure 2). The difference could come from the difference in the working electric current for the positive and reversed emitting electrodes electrospinning setups. It was noted that the electric current of the jets for the reversed emitting electrode approach is about 0.3–0.5 μA much lower than that of the positive emitting electrode approach (4.6–5.4 μA). A lower working electric current corresponds to a lower charge density of the jet flow during electrospinning [33–35]. As a result, the electric field force stretching the jet as well as the electrostatic repulsion between the jets is accordingly smaller, leading to the relatively low stretching force on the jet and the thick diameter of the resultant nanofibers. TEM observation indicates that the nanofibers prepared by both positive and reversed emitting electrodes electrospinning setups have uniform structure (Figure 3), which suggests that NAP molecules are homogeneously dispersed in the CA matrixes.Typical SEM images and corresponding diameter distribution of the nanofibers prepared by (a) positive and (b) reversed emitting electrodes electrospinning setups.
(a)
(b)TEM images of nanofibers prepared by (a) positive and (b) reversed emitting electrodes electrospinning setups.
(a)
(b)


### 3.2. Dispersion of the NAP in the CA Matrix

The solubility of hydrophobic drugs, such as NAP, is quite important for the improvement of the drug efficiency, which can be achieved by adding additives to reduce the crystallization of the drugs. Figure4 shows the DSC and XRD curves of the NAP/CA hybrid nanofibers prepared by positive and reversed emitting electrodes electrospinning setups. Data of NAP and CA are also provided for comparison. The DSC results indicate that there is no melting peak of NAP crystal appearing on both NAP/CA nanofibers, suggesting no NAP crystal formed in either of the nanofibers. The XRD results further confirmed that no NAP crystal exists in the nanofibers. These results are consistent with the TEM observations (Figure 3), in which no NAP crystal or aggregation can be observed.DSC traces (a) and XRD curves (b) of the NAP/CA nanofibers and correlated raw materials.
(a)
(b)For clarification of the interactions between the NAP and the CA matrix, FTIR experiments were carried out and the results are shown in Figure5. The well-defined sharp absorption peak of pure NAP is at 1728 cm−1, which comes from the stretching vibration of the carbonyl groups. The absorption peak of carbonyl groups of pure CA locates at 1753 cm−1. For both hybrid nanofibers, the absorption peak of carbonyl groups locates at 1743 cm−1, indicating the interactions between the carbonyl groups from NAP and CA molecules [29, 36, 37]. Moreover, on the FTIR spectrum of NAP, the strong broad absorption peak at around 3150 cm−1 is from the hydroxyl groups of NAP dimers as indicated in Figure 6. This peak disappeared in both hybrid nanofibers. Meanwhile, the absorption peak from the –OH groups in CA at around 3520 cm−1red shifts to around 3470 cm−1 due to the formation of hydrogen bonds between NAP and CA molecules. Above results confirm that NAP mixed with CA in molecular level in the resultant NAP/CA hybrid nanofibers. The interactions between NAP and CA molecules can efficiently prevent the crystallization of NAP, which is helpful for the enhancement of the solubility of NAP.Figure 5
FTIR spectra of NAP-loaded nanofibers and correlated raw materials.Figure 6
The hydrogen bonding of NAP dimer and NAP/CA.


### 3.3. Distribution of NAP in the CA Matrix

NAP contents in the hybrid nanofibers prepared by positive and reversed emitting electrodes electrospinning setups are similar to each other. The actual NAP content is 8.89 wt% and 8.94 wt% for nanofibers prepared by positive and reversed emitting electrodes electrospinning setups, respectively. The data are similar to the feeding NAP content in the NAP/CA solution (9.09 wt%), indicating that there is no NAP loss during the preparation of the nanofibers. The in vitro NAP release profiles of the nanofibers are shown in Figure7. Three parallel experiments were carried out and the average data and the error bar are depicted. The results showed that the NAP release of the nanofibers prepared by reversed emitting electrode electrospinning setup is much faster than that of the nanofibers prepared by the positive emitting electrode electrospinning setup (Figure 2). As reported in the literatures, the release rate of the same drug from electrospun nanofibers of the same matrix depends on the diameter of the nanofibers. The drug releasing rate of the nanofibers with thicker diameter is slower than that of the nanofibers with thinner diameter [29, 36, 37]. However, in the present work, the drug releasing rate of the NAP/CA nanofibers (diameter of 574.4±212.2 nm) prepared from the positive emitting electrode approach is slower than that of the nanofibers (diameter of 594.6±197.2 nm) prepared from the reversed emitting electrode approach (Figure 7), which is different from the literatures in [29, 36, 37]. The difference in the NAP release behaviors of the NAP/CA hybrid nanofibers suggests that the distribution of NAP in the nanofibers prepared by the positive and reversed emitting electrodes electrospinning approaches could be different. The fast NAP release of the nanofibers prepared by reversed emitting electrode electrospinning setup could be attributed to the fact that the NAP molecules were enriched near the surface of the nanofibers during the electrospinning. In order to approve the difference in the content of NAP near the surface of the nanofibers, EDS experiments were carried out and the data are listed in Table 1. It was found that the oxygen content in fibers prepared from the positive emitting electrode is higher than that in the nanofibers prepared from reversed approach. Considering that EDS provides exact element compositions of the nanofiber surface within depth of 5–10 nm and that the oxygen content in CA is higher than that in NAP (Table 1), the EDS results suggested that NAP was enriched near the surface of the nanofibers prepared from the reversed emitting electrode, which is consistent with the conclusion derived from the NAP release profile of both nanofibers.Table 1
Element C and O in different fibers and the raw materials.
Materials
C/wt%
O/wt%
CA*
51.72
4828
NAP*
77.78
22.22
Fibers from positive setup
67.92
32.08
Fibers from reversed setup
80.34
19.66
*The weight fraction was calculated from its molecular formula.Figure 7
In vitro NAP release of the NAP/CA hybrid nanofibers prepared by positive and reversed emitting electrodes electrospinning setups.NAP molecules have carboxyl groups and could be ionized in CA/acetone/DMAc solution (Figure8). The conductivities of the CA and NAP/CA solutions with acetone/DMAc mixed solvent are 0.765 and 0.857 mS/m, respectively, confirming the ionization of NAP molecules in the mixed solution. During the electrospinning process, the electric field enhanced the dissociation and any structure capable of being ionized would experience a separation of charges [38]. With a positive emitting electrode electrospinning setup, the reversed ions will migrate to the inner surface of the needle and vice versa, resulting in the core part of the solution fluid with an excess of mobile charges that can respond to the electrostatic stress of the opposite electrode. In the case of the electrospinning of the NAP/CA solution with positive emitting electrode setup, the electron with smaller mass moves to the needle surface much faster than the proton with bigger mass in the case of reversed emitting electrode electrospinning setup (Figure 9), resulting in the positive flying jet with more net charges than the reversed jet, which is confirmed by the difference in the working electric current during the positive (4.6–5.4 μA) and reversed (0.3–0.5 μA) emitting electrodes electrospinning in the present work. It is widely accepted that the net charge moves to the surface of the flying jet and the drift velocity of ions inside the jet is about 0.1 m/s [33, 39, 40]. Therefore, the negatively charged NAP could move to the surface of the jet in the reversed emitting electrode electrospinning, which leads to the higher NAP content near the surface of the prepared nanofibers. Therefore, the release rate of the NAP is faster from the nanofibers prepared by reversed emitting electrode electrospinning setup than from those nanofibers prepared by positive emitting electrode electrospinning setup. Considering the distribution of the loaded NAP caused by the emitting electrode polarity, drugs of different ionization properties can be embedded in the polymer matrixes of nanofibers with relatively controlled distribution by modulating the emitting electode polarity, which could be used to prepare biomedical materials for various applications, such as wound dressings and surgical scaffolds.Figure 8
The ionization of NAP in the solution.NAP distribution in the jets of (a) positive and (b) reversed emitting electrodes electrospinning setups.
(a)
(b)


## 3.1. Preparation of NAP/CA Nanofibers

NAP/CA hybrid nanofiber mats can be successfully prepared by both positive and reversed emitting electrode setups (Figure S1); see Figure S1 in Supplementary Material available online athttp://dx.doi.org/10.1155/2014/360658. Figure 2 shows the typical SEM images of the NAP/CA nanofibers prepared by electrospinning using positive and reversed emitting electrodes. The results indicate that the resultant nanofibers have similar morphology. The nanofibers have smooth surfaces and no particles can be observed, suggesting no NAP aggregations formed outside of the hybrid nanofibers. Statistics of the diameter of the nanofibers indicates that the nanofibers prepared by reversed emitting electrode electrospinning have a larger average diameter and a narrower diameter distribution (594.6±197.2 nm) compared with those nanofibers prepared by using positive emitting electrode setup (574.4±212.2 nm) (Figure 2). The difference could come from the difference in the working electric current for the positive and reversed emitting electrodes electrospinning setups. It was noted that the electric current of the jets for the reversed emitting electrode approach is about 0.3–0.5 μA much lower than that of the positive emitting electrode approach (4.6–5.4 μA). A lower working electric current corresponds to a lower charge density of the jet flow during electrospinning [33–35]. As a result, the electric field force stretching the jet as well as the electrostatic repulsion between the jets is accordingly smaller, leading to the relatively low stretching force on the jet and the thick diameter of the resultant nanofibers. TEM observation indicates that the nanofibers prepared by both positive and reversed emitting electrodes electrospinning setups have uniform structure (Figure 3), which suggests that NAP molecules are homogeneously dispersed in the CA matrixes.Typical SEM images and corresponding diameter distribution of the nanofibers prepared by (a) positive and (b) reversed emitting electrodes electrospinning setups.
(a)
(b)TEM images of nanofibers prepared by (a) positive and (b) reversed emitting electrodes electrospinning setups.
(a)
(b)


## 3.2. Dispersion of the NAP in the CA Matrix

The solubility of hydrophobic drugs, such as NAP, is quite important for the improvement of the drug efficiency, which can be achieved by adding additives to reduce the crystallization of the drugs. Figure4 shows the DSC and XRD curves of the NAP/CA hybrid nanofibers prepared by positive and reversed emitting electrodes electrospinning setups. Data of NAP and CA are also provided for comparison. The DSC results indicate that there is no melting peak of NAP crystal appearing on both NAP/CA nanofibers, suggesting no NAP crystal formed in either of the nanofibers. The XRD results further confirmed that no NAP crystal exists in the nanofibers. These results are consistent with the TEM observations (Figure 3), in which no NAP crystal or aggregation can be observed.DSC traces (a) and XRD curves (b) of the NAP/CA nanofibers and correlated raw materials.
(a)
(b)For clarification of the interactions between the NAP and the CA matrix, FTIR experiments were carried out and the results are shown in Figure5. The well-defined sharp absorption peak of pure NAP is at 1728 cm−1, which comes from the stretching vibration of the carbonyl groups. The absorption peak of carbonyl groups of pure CA locates at 1753 cm−1. For both hybrid nanofibers, the absorption peak of carbonyl groups locates at 1743 cm−1, indicating the interactions between the carbonyl groups from NAP and CA molecules [29, 36, 37]. Moreover, on the FTIR spectrum of NAP, the strong broad absorption peak at around 3150 cm−1 is from the hydroxyl groups of NAP dimers as indicated in Figure 6. This peak disappeared in both hybrid nanofibers. Meanwhile, the absorption peak from the –OH groups in CA at around 3520 cm−1red shifts to around 3470 cm−1 due to the formation of hydrogen bonds between NAP and CA molecules. Above results confirm that NAP mixed with CA in molecular level in the resultant NAP/CA hybrid nanofibers. The interactions between NAP and CA molecules can efficiently prevent the crystallization of NAP, which is helpful for the enhancement of the solubility of NAP.Figure 5
FTIR spectra of NAP-loaded nanofibers and correlated raw materials.Figure 6
The hydrogen bonding of NAP dimer and NAP/CA.


## 3.3. Distribution of NAP in the CA Matrix

NAP contents in the hybrid nanofibers prepared by positive and reversed emitting electrodes electrospinning setups are similar to each other. The actual NAP content is 8.89 wt% and 8.94 wt% for nanofibers prepared by positive and reversed emitting electrodes electrospinning setups, respectively. The data are similar to the feeding NAP content in the NAP/CA solution (9.09 wt%), indicating that there is no NAP loss during the preparation of the nanofibers. The in vitro NAP release profiles of the nanofibers are shown in Figure7. Three parallel experiments were carried out and the average data and the error bar are depicted. The results showed that the NAP release of the nanofibers prepared by reversed emitting electrode electrospinning setup is much faster than that of the nanofibers prepared by the positive emitting electrode electrospinning setup (Figure 2). As reported in the literatures, the release rate of the same drug from electrospun nanofibers of the same matrix depends on the diameter of the nanofibers. The drug releasing rate of the nanofibers with thicker diameter is slower than that of the nanofibers with thinner diameter [29, 36, 37]. However, in the present work, the drug releasing rate of the NAP/CA nanofibers (diameter of 574.4±212.2 nm) prepared from the positive emitting electrode approach is slower than that of the nanofibers (diameter of 594.6±197.2 nm) prepared from the reversed emitting electrode approach (Figure 7), which is different from the literatures in [29, 36, 37]. The difference in the NAP release behaviors of the NAP/CA hybrid nanofibers suggests that the distribution of NAP in the nanofibers prepared by the positive and reversed emitting electrodes electrospinning approaches could be different. The fast NAP release of the nanofibers prepared by reversed emitting electrode electrospinning setup could be attributed to the fact that the NAP molecules were enriched near the surface of the nanofibers during the electrospinning. In order to approve the difference in the content of NAP near the surface of the nanofibers, EDS experiments were carried out and the data are listed in Table 1. It was found that the oxygen content in fibers prepared from the positive emitting electrode is higher than that in the nanofibers prepared from reversed approach. Considering that EDS provides exact element compositions of the nanofiber surface within depth of 5–10 nm and that the oxygen content in CA is higher than that in NAP (Table 1), the EDS results suggested that NAP was enriched near the surface of the nanofibers prepared from the reversed emitting electrode, which is consistent with the conclusion derived from the NAP release profile of both nanofibers.Table 1
Element C and O in different fibers and the raw materials.
Materials
C/wt%
O/wt%
CA*
51.72
4828
NAP*
77.78
22.22
Fibers from positive setup
67.92
32.08
Fibers from reversed setup
80.34
19.66
*The weight fraction was calculated from its molecular formula.Figure 7
In vitro NAP release of the NAP/CA hybrid nanofibers prepared by positive and reversed emitting electrodes electrospinning setups.NAP molecules have carboxyl groups and could be ionized in CA/acetone/DMAc solution (Figure8). The conductivities of the CA and NAP/CA solutions with acetone/DMAc mixed solvent are 0.765 and 0.857 mS/m, respectively, confirming the ionization of NAP molecules in the mixed solution. During the electrospinning process, the electric field enhanced the dissociation and any structure capable of being ionized would experience a separation of charges [38]. With a positive emitting electrode electrospinning setup, the reversed ions will migrate to the inner surface of the needle and vice versa, resulting in the core part of the solution fluid with an excess of mobile charges that can respond to the electrostatic stress of the opposite electrode. In the case of the electrospinning of the NAP/CA solution with positive emitting electrode setup, the electron with smaller mass moves to the needle surface much faster than the proton with bigger mass in the case of reversed emitting electrode electrospinning setup (Figure 9), resulting in the positive flying jet with more net charges than the reversed jet, which is confirmed by the difference in the working electric current during the positive (4.6–5.4 μA) and reversed (0.3–0.5 μA) emitting electrodes electrospinning in the present work. It is widely accepted that the net charge moves to the surface of the flying jet and the drift velocity of ions inside the jet is about 0.1 m/s [33, 39, 40]. Therefore, the negatively charged NAP could move to the surface of the jet in the reversed emitting electrode electrospinning, which leads to the higher NAP content near the surface of the prepared nanofibers. Therefore, the release rate of the NAP is faster from the nanofibers prepared by reversed emitting electrode electrospinning setup than from those nanofibers prepared by positive emitting electrode electrospinning setup. Considering the distribution of the loaded NAP caused by the emitting electrode polarity, drugs of different ionization properties can be embedded in the polymer matrixes of nanofibers with relatively controlled distribution by modulating the emitting electode polarity, which could be used to prepare biomedical materials for various applications, such as wound dressings and surgical scaffolds.Figure 8
The ionization of NAP in the solution.NAP distribution in the jets of (a) positive and (b) reversed emitting electrodes electrospinning setups.
(a)
(b)


## 4. Conclusions

NAP/CA hybrid nanofibers were prepared by both positive and reversed emitting electrodes electrospinning setups. The nanofibers prepared by reversed emitting electrode electrospinning setup have relatively thicker diameter than those prepared by positive emitting electrode electrospinning setup. NAP is dispersed in the CA matrix in molecular level without formation of NAP aggregation and dimer. Nanofibers prepared by the reversed emitting electrode electrospinning setup have a larger amount of NAP in the surface layer, which resulted in the faster NAP release compared with the nanofibers prepared by positive emitting electrode electrospinning setup. This finding is important in producing hybrid nanofibers with drugs of different ionization property through blend electrospinning, which may have various drug release behaviors and then be used as biomedical materials.



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*Source: 360658-2014-05-04.xml*