# Resonance Two-Photon Ionization of Diarylethene in the Presence of Cyclodextrin

Authors: Michihiro Hara; Makoto Miwa; Tatsuya Takeshita; Shouhei Watanabe
Journal: International Journal of Photoenergy
 (2013)

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## Abstract

The transient absorption of the diarylethene 1,2-bis(2,4-dimethyl-5-phenyl-3-thienyl)-3,3,4,4,5,5-hexafluoro-1-cyclopentene (DE) was measured using 266 nm laser flash photolysis in the presence of various cyclodextrins (CDs) in aqueous solvent. Ionization of DE occurred within the laser flash (5 ns) to give the DE radical cation and water-solvated electron. The electron was generated by two-photon excitation through simultaneous irradiation with 266 nm laser light, and the ionization quantum yields of DE in the presence ofα-CD, β-CD, m-β-CD, and γ-CD depended on the properties of the DE/CD complexes. These results suggest that useful two-photon ionization occurred for these complexes. The ionization quantum yield of DE was found to increase in aqueous solution after the addition of m-β-CD (50 mM), in which m-β-CD likely controlled the photochromic reactivity of DE.


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## Body

## 1. Introduction

Research on the phenomenon of photochromism of organic compounds has advanced in recent years. Representative compounds with this property include azobenzene and spiropyrans [1, 2]. The diarylethene, 1,2-bis(2,4-dimethyl-5-phenyl-3-thienyl)-3,3,4,4,5,5-hexafluoro-1-cyclopentene, (DE) (Figure 1) used in our study also exhibits this property. Photochromism is a phenomenon where irradiation with certain wavelengths of light induces a reversible transformation between two structures without a change in molecular weight. Substances with this property are referred to as photochromic materials. Diarylethenes are superior to other photochromic materials in terms of thermal stability, high sensitivity, and durability with respect to repeated transformation. In addition, by introducing various substituents at the aryl position, the absorption spectrum can be changed to obtain different colors upon irradiation with ultraviolet light. These materials are expected to find uses in various applications, such as in memory devices in the IT field, as sensor materials for light modulation, or in the industrial field as well as in textile products (printed shirts). Due to the poor solubility of this DE in water, we used the inclusion effect of cyclodextrins (CDs) to dissolve it in water, after which we investigated its photoionization properties [2–16], which is a type of photolysis. The photochromic behavior of diarylethene derivatives attached to CD and dissolved in water has been reported to influence the quantum yield of the transformation from an open to a closed structure [17, 18]. There are several varieties of CD depending on the number of glucose units, including the α (6 units), β (7 units), and γ (8 units) forms. A characteristic feature of CDs is their ability to form inclusion structures, whose vacant internal spaces display a hydrophobic nature, while the exterior is hydrophilic. Consequently, organic compounds in water can be dissolved by inserting them into the internal CD vacant space.Figure 1
Reaction scheme of DE.While CD inclusion has been used to study the photochromic behavior of DE species in water, the effect of this inclusion on photoionization has not been reported. Because of their different cavity sizes, each of the CD species can accommodate a different portion of the DE molecule, which serves as a way to tune the local environment surrounding the DE. As more of the DE is included, its local environment becomes increasingly hydrophobic, which allows us to elucidate the effect of changing the environment on the ionization process.In the present research, DE was dissolved in water using the inclusion effect of CD, after which we examined its resonant two-photon ionization TPI [3] by laser flash photolysis (LFP), where the sample was irradiated with light at a wavelength of 266 nm. From the observed ΔO.D. of the hydration electrons, we calculated the ionization quantum yield (Φion) and examined the mechanism of TPI. We also conducted a comparative examination of the fluorescence lifetime (τ) since it is known to depend on Φion.


## 2. Experimental

We prepared aqueous solutions of the diarylethene 1,2-bis(2,4-dimethyl-5-phenyl-3-thienyl)-3,3,4,4,5,5-hexafluoro-1-cyclopentene (Tokyo Kasei) with various cyclodextrin varieties. Acetonitrile (spectroscopic grade) was purchased from Wako and benzophenone was purchased from Tokyo Kasei.α-CD and β-CD were purchased from Nacalai Tesque, 6-O-α-D-glucosyl-β-cyclodextrin (m-β-CD) was obtained from Tokyo Kasei, and γ-CD was purchased from Kanto Chemical Co. Each of the DE/CD solutions were prepared in a 9 : 1 (v/v) water/acetonitrile (ACN) mixture. All the commercially available materials were used as received with no further purification. The DE concentration used for each study is indicated throughout the text, and the CD concentration was either 10 or 50 mM; because of its low solubility, we were not able to prepare a 50 mM β-CD solution. The DE solution and CD water solution were mixed simply, and the transparent samples were prepared.The absorption and fluorescence spectra of the prepared samples were measured with a spectrophotometer (Hitachi, U-3310) and a fluorescence spectrophotometer (Perkin Elmer, LS55), respectively, and the fluorescence lifetime was measured with a fluorescence lifetime measurement device (Hamamatsu, C11369). The fluorescence lifetimes of DE were estimated with deconvolution analysis (Hamamatsu, U11487). In addition, single-wavelength irradiation was performed with a quadrupled YAG laser (266 nm, 45 mJ, 5 ns pulse duration; Spectra-Physics GCR-130-10). Benzophenone was used as an actinometer for measuring the photon concentration. To observe the transient absorption (ΔO.D.) of the hydration electrons, the wavelength for observation was set to 720 nm. The obtained hydration electron density ([eaq-]′) was used to calculate Φion as follow:
(1)2Φion=[eaq-]′[photon]=(ΔO.D.720/(ε720×l))/Abs266DE(ΔO.D.525/(ε525×l))/Abs266Bp=[eaq-]/Abs266DE[Bp*3]/Abs266Bp.
Taking the triplet concentration [Bp*3] of benzophenone (Bp) as the denominator and the concentration of hydration electrons [eaq-] as the numerator, ΔO.D.720 denotes ΔO.D. and ε720 denotes ε720 = 1.85 × 104 M−1 cm−1 for the hydration electrons. Abs266DE is the absorbance at 266 nm of the solute sample when using LFP, ΔO.D.525 denotes ΔO.D. of Bp triplets, and ε525 denotes ε525 = 6500 M−1 cm−1 of Bp. Furthermore, Abs266Bp denotes the absorbance of the Bp sample at 266 nm when LFP is used, and the factor 2 in 2Φion accounts for the absorption of two photons.The ionization potential (IP) of DE was calculated on the basis of cyclic voltammetry (CV) measurements with ACN as solvent and DE as the solute. We used 0.1 M tetrabutylammonium as the electrolyte and an Ag/AgNO3 reference electrode (10 mM).To estimate the extent of inclusion, each of the DE/CD complexes were modeled and subjected to geometry optimization to the most energetically stable structure using MM2 (CS Chem3D). Fully included CD structures were used as starting geometries.1H NMR spectra were measured on a 400 MHz spectrometer (JEOL DATUM JNM-ATUM]) with the same concentrations used in the laser flash photolysis studies.


## 3. Results and Discussion

### 3.1. Structure of the DE/CD Complexes

The most stable, minimum-energy structures, formed through inclusion of DE into CD, obtained from the results of MM2 calculations are shown in Figure2, and these results were used to examine the structural differences between each of the CD complexes. The inner diameters of α-CD, β-CD, m-β-CD, and γ-CD are 4.5, 7.0, 7.0, and 8.5 Å, respectively, and the depth of the ring in all cases is 7.0 Å. The length of DE is 16.0 Å along the long axis and 6.4 Å along the short axis, and the length of the photoisomer of DE is 14.0 Å along the long axis and 7.0 Å along the short axis. The calculation results indicate that in the α-CD inclusion complex only one of the DE benzene rings inserts into the CD, while the hexafuluoro-cyclopentene group protrudes into the solution. The calculations indicate that the DE embeds deeper into the vacant space of β-CD and m-β-CD than with α-CD. Furthermore, γ-CD includes DE either fully (all the way into the center) or partially (including only the benzene rings of two DE molecules). With regard to the ionization process, when DE can be easily stabilized by solvent molecules, ionization is more efficient and the ionization quantum yield is likely to increase. Therefore, of all the complexes, the α-CD complex should be the easiest to photoionize because a large portion of the DE protrudes into the solvent.Figure 2
Minimum-energy structures of DE/CD complexes calculated by MM2.The open form structure of DE, which exhibits photoisomerism, exists in a parallel state and an antiparallel state [17, 18]. The 1H NMR spectrum of DE (6.1 × 10−3 M, 20°C, 400 MHz) in deuterated ACN (no CD) showed peaks at δ 2.38 and 2.40 ppm which were assigned to the methyl protons at the 4 positions on the thiophene ring for the DE in the parallel and antiparallel conformations, respectively. The intensity ratio of the doublet peak indicates that the two DE conformations exist in almost equal populations in deuterated ACN. At our experimental solution concentrations, the methyl protons at the 2 positions on the DE thiophene ring expected around δ 2.0 were not observed [19].DE (6.1 × 10−3 M, 20°C) was then suspended in the absence of CD in H2O/ACN (9 : 1). No significant NMR spectral peaks were observed for DE (6.1 × 10−7 M) at the LFP condition at around 2.4 ppm, which is the overlap signal of the methyl protons at the 4 positions on the thiophene ring for the DE and H2O (δ 2.1) in deuterium ACN. This is most likely because of the low DE concentration used in the experiments. Therefore, confirmation of the formation of the DE/CD inclusion complexes was taken from shifts in the peak of the fluorescence spectra from 476 nm for DE solutions with no CD to 320–420 nm for DE/CD solutions (see Figure 3) as well as changes in the fluorescence lifetime profiles (see Figure 5, described later) [18].Figure 3
Absorption (black line) and fluorescence spectra of DE in the absence (red line, no CD, pure ACN solvent) and presence ofα-CD (green), β-CD (blue), m-β-CD (cyan), and γ-CD (magenta). All CD solutions were [10 mM] in H2O/ACN (9 : 1).


### 3.2. Measurement of Absorption and Fluorescence Spectra and Fluorescence Lifetime

We measured the absorption and fluorescence spectra of aqueous solutions of DE (6.1 × 10−7 M) in H2O/ACN (9 : 1) in the presence of CD, shown in Figure 3, for each of the CD species. As an example, we consider the results for the DE/α-CD inclusion complex. The maximum absorption was at 271 nm (Figure 3, black line), and the maximum fluorescence was at 421 nm (Figure 3, green line). Taking the wavelength at the maximum fluorescence, the singlet excitation energy (S1) was calculated to be 3.0 eV. Furthermore, cyclic voltammetry indicated a redox potential E1/2ox of 0.6 eV, and the ionization potential (IP) of DE in aqueous solution was calculated to be 6.7 eV, using the conversion formula [20]. Next, we measured the fluorescence lifetimes (τ) of the CD/DE complexes in the 9 : 1 H2O/ACN solutions. Representative spectra are shown in Figure 4, and a summary of the results for all systems is presented in Table 1. The measured τ consisted of three components τ1, τ2, and τ3, which were assumed to correspond to open ring parallel forms of DE only partially included within the CD (OP); closed ring, antiparallel forms of partially-included DE in CD (CL); and inclusion complexes, respectively. We note that the closed form could be generated by the excitation laser in the lifetime measurement; however, no solution color change was observed. Our assignment of the lifetime components is based on the reports of Irie et al. that the addition of CD to an aqueous solution of the open-ring form DE increased the abundance of the antiparallel conformation [17, 18]. With the addition of CD (from 10 to 50 mM), the fluorescence lifetimes of DE were affected (see Table 1). The A2 factor increased and the A1 factor decreased when increasing the CD concentration. Hence, the A1 and A2 factors should be derived from the parallel and antiparallel conformations. Because it is 100-fold longer than the single-component fluorescence lifetime of pure DE in organic solvent [21], we have assigned is the τ3 component to the inclusion complex.Table 1
Ionization quantum yields and fluorescence lifetimes of DE (6.1 × 10−5 M) in the presence of α-CD (10−3 or 5 × 10−3 M), β-CD (10−3 M), m-β-CD (10−3 or 5 × 10−3 M), or γ-CD (10−3 or 5 × 10−3 M) in H2O/ACN (9 : 1).
CD
mM
τ
1 (ns)
τ
2 (ns)
τ
3 (ns)
A
1 (%)
A
2 (%)
A
3 (%)
CHI
Φ
ion (%)
α-CD
10
0.3
0.5
9.7
80
16
4
1.06
0.8
α-CD
50
0.4
2.8
16
67
26
7
1.03
0.6
β-CD
10
0.7
3.7
32
72
26
2
1.00
0.8
mβ-CD
10
0.5
3.7
19
90
9
1
1.04
0.6
mβ-CD
50
1.0
3.7
20
51
44
5
1.01
1.1
γ-CD
10
0.7
4.4
33
91
8
1
1.01
0.9
γ-CD
50
0.8
3.8
28
89
10
1
1.02
0.7Figure 4
Energy-level diagram for DE in the ground state and excited state.Figure 5
Representative time profiles of fluorescence lifetimes of DE in the absence (black line) and the presence ofα-CD (10 mM, red line; 50 mM, green line), or mβ-CD (10 mM, blue line; 50 mM, cyan line) in ACN or H2O/ACN (9 : 1), respectively.Here,Φion is considered to depend on the existence probability of the second photon, and the fluorescence lifetime is one of the important physical measures in this respect. Looking at the values of the fluorescence lifetime of DE, longer fluorescence lifetimes are considered to be associated with ionization. Comparing τ1, τ2, and τ3 of DE in the presence of each CD, τ1 was longer (1.0 ns) and the ionization quantum yield was higher when 50 mM of m-β-CD was added.


### 3.3. Two-Photon Ionization of DE

Hydration electrons (720 nm) were observed upon irradiation with 266 nm (45 mJ) laser light of the (9 : 1) H2O/ACN DE/CD complex solutions, which indicates ionization of the DE molecule. Since ionization to IP (6.7 eV) is impossible to achieve with the energy of a single photon (266 nm, 4.7 eV), it was implied that ionization is induced by two or more photons (Figure 5).The values ofΦion for the various DE/CD complex solutions are shown in Table 1. The Φion value for α-CD (10 mM) and β-CD (10 mM) was the same (0.8%). However, the fluorescence lifetime values of τ1, τ2, and τ3 were longer for β-CD, changing from 0.3 ns to 0.7 ns, from 0.7 ns to 3.7 ns, and from 9.7 ns to 32 ns, respectively. In the same manner, a comparison for m-β-CD and γ-CD at 10 mM revealed no dependence on the fluorescence lifetime.Regarding the concentration effect of each CD variety,Φion for α-CD and γ-CD decreased by about 20% as the concentration increased. This is considered to be due to the increased difficulty of receiving the solvation stabilization energy.However, in the case of m-β-CD, in addition to τ1 becoming longer (from 0.5 ns to 1.0 ns), Φion also increased from 0.6% to 1.1%. This increase is partially because the amount of DE inclusion in CD increases at high concentrations, but also because when the DE is included on the maltose side (Figure 6), or if the DE and CD form a 1 : 2 complex, DE isomerization immediately after irradiation is blocked. This suppresses the photochromic reaction transforming the open form to closed form, and, as a result, the average lifetime becomes longer and TPI occurs more readily.Figure 6
Minimum-energy structure of the DE/m-β-CD complex calculated by MM2.


## 3.1. Structure of the DE/CD Complexes

The most stable, minimum-energy structures, formed through inclusion of DE into CD, obtained from the results of MM2 calculations are shown in Figure2, and these results were used to examine the structural differences between each of the CD complexes. The inner diameters of α-CD, β-CD, m-β-CD, and γ-CD are 4.5, 7.0, 7.0, and 8.5 Å, respectively, and the depth of the ring in all cases is 7.0 Å. The length of DE is 16.0 Å along the long axis and 6.4 Å along the short axis, and the length of the photoisomer of DE is 14.0 Å along the long axis and 7.0 Å along the short axis. The calculation results indicate that in the α-CD inclusion complex only one of the DE benzene rings inserts into the CD, while the hexafuluoro-cyclopentene group protrudes into the solution. The calculations indicate that the DE embeds deeper into the vacant space of β-CD and m-β-CD than with α-CD. Furthermore, γ-CD includes DE either fully (all the way into the center) or partially (including only the benzene rings of two DE molecules). With regard to the ionization process, when DE can be easily stabilized by solvent molecules, ionization is more efficient and the ionization quantum yield is likely to increase. Therefore, of all the complexes, the α-CD complex should be the easiest to photoionize because a large portion of the DE protrudes into the solvent.Figure 2
Minimum-energy structures of DE/CD complexes calculated by MM2.The open form structure of DE, which exhibits photoisomerism, exists in a parallel state and an antiparallel state [17, 18]. The 1H NMR spectrum of DE (6.1 × 10−3 M, 20°C, 400 MHz) in deuterated ACN (no CD) showed peaks at δ 2.38 and 2.40 ppm which were assigned to the methyl protons at the 4 positions on the thiophene ring for the DE in the parallel and antiparallel conformations, respectively. The intensity ratio of the doublet peak indicates that the two DE conformations exist in almost equal populations in deuterated ACN. At our experimental solution concentrations, the methyl protons at the 2 positions on the DE thiophene ring expected around δ 2.0 were not observed [19].DE (6.1 × 10−3 M, 20°C) was then suspended in the absence of CD in H2O/ACN (9 : 1). No significant NMR spectral peaks were observed for DE (6.1 × 10−7 M) at the LFP condition at around 2.4 ppm, which is the overlap signal of the methyl protons at the 4 positions on the thiophene ring for the DE and H2O (δ 2.1) in deuterium ACN. This is most likely because of the low DE concentration used in the experiments. Therefore, confirmation of the formation of the DE/CD inclusion complexes was taken from shifts in the peak of the fluorescence spectra from 476 nm for DE solutions with no CD to 320–420 nm for DE/CD solutions (see Figure 3) as well as changes in the fluorescence lifetime profiles (see Figure 5, described later) [18].Figure 3
Absorption (black line) and fluorescence spectra of DE in the absence (red line, no CD, pure ACN solvent) and presence ofα-CD (green), β-CD (blue), m-β-CD (cyan), and γ-CD (magenta). All CD solutions were [10 mM] in H2O/ACN (9 : 1).


## 3.2. Measurement of Absorption and Fluorescence Spectra and Fluorescence Lifetime

We measured the absorption and fluorescence spectra of aqueous solutions of DE (6.1 × 10−7 M) in H2O/ACN (9 : 1) in the presence of CD, shown in Figure 3, for each of the CD species. As an example, we consider the results for the DE/α-CD inclusion complex. The maximum absorption was at 271 nm (Figure 3, black line), and the maximum fluorescence was at 421 nm (Figure 3, green line). Taking the wavelength at the maximum fluorescence, the singlet excitation energy (S1) was calculated to be 3.0 eV. Furthermore, cyclic voltammetry indicated a redox potential E1/2ox of 0.6 eV, and the ionization potential (IP) of DE in aqueous solution was calculated to be 6.7 eV, using the conversion formula [20]. Next, we measured the fluorescence lifetimes (τ) of the CD/DE complexes in the 9 : 1 H2O/ACN solutions. Representative spectra are shown in Figure 4, and a summary of the results for all systems is presented in Table 1. The measured τ consisted of three components τ1, τ2, and τ3, which were assumed to correspond to open ring parallel forms of DE only partially included within the CD (OP); closed ring, antiparallel forms of partially-included DE in CD (CL); and inclusion complexes, respectively. We note that the closed form could be generated by the excitation laser in the lifetime measurement; however, no solution color change was observed. Our assignment of the lifetime components is based on the reports of Irie et al. that the addition of CD to an aqueous solution of the open-ring form DE increased the abundance of the antiparallel conformation [17, 18]. With the addition of CD (from 10 to 50 mM), the fluorescence lifetimes of DE were affected (see Table 1). The A2 factor increased and the A1 factor decreased when increasing the CD concentration. Hence, the A1 and A2 factors should be derived from the parallel and antiparallel conformations. Because it is 100-fold longer than the single-component fluorescence lifetime of pure DE in organic solvent [21], we have assigned is the τ3 component to the inclusion complex.Table 1
Ionization quantum yields and fluorescence lifetimes of DE (6.1 × 10−5 M) in the presence of α-CD (10−3 or 5 × 10−3 M), β-CD (10−3 M), m-β-CD (10−3 or 5 × 10−3 M), or γ-CD (10−3 or 5 × 10−3 M) in H2O/ACN (9 : 1).
CD
mM
τ
1 (ns)
τ
2 (ns)
τ
3 (ns)
A
1 (%)
A
2 (%)
A
3 (%)
CHI
Φ
ion (%)
α-CD
10
0.3
0.5
9.7
80
16
4
1.06
0.8
α-CD
50
0.4
2.8
16
67
26
7
1.03
0.6
β-CD
10
0.7
3.7
32
72
26
2
1.00
0.8
mβ-CD
10
0.5
3.7
19
90
9
1
1.04
0.6
mβ-CD
50
1.0
3.7
20
51
44
5
1.01
1.1
γ-CD
10
0.7
4.4
33
91
8
1
1.01
0.9
γ-CD
50
0.8
3.8
28
89
10
1
1.02
0.7Figure 4
Energy-level diagram for DE in the ground state and excited state.Figure 5
Representative time profiles of fluorescence lifetimes of DE in the absence (black line) and the presence ofα-CD (10 mM, red line; 50 mM, green line), or mβ-CD (10 mM, blue line; 50 mM, cyan line) in ACN or H2O/ACN (9 : 1), respectively.Here,Φion is considered to depend on the existence probability of the second photon, and the fluorescence lifetime is one of the important physical measures in this respect. Looking at the values of the fluorescence lifetime of DE, longer fluorescence lifetimes are considered to be associated with ionization. Comparing τ1, τ2, and τ3 of DE in the presence of each CD, τ1 was longer (1.0 ns) and the ionization quantum yield was higher when 50 mM of m-β-CD was added.


## 3.3. Two-Photon Ionization of DE

Hydration electrons (720 nm) were observed upon irradiation with 266 nm (45 mJ) laser light of the (9 : 1) H2O/ACN DE/CD complex solutions, which indicates ionization of the DE molecule. Since ionization to IP (6.7 eV) is impossible to achieve with the energy of a single photon (266 nm, 4.7 eV), it was implied that ionization is induced by two or more photons (Figure 5).The values ofΦion for the various DE/CD complex solutions are shown in Table 1. The Φion value for α-CD (10 mM) and β-CD (10 mM) was the same (0.8%). However, the fluorescence lifetime values of τ1, τ2, and τ3 were longer for β-CD, changing from 0.3 ns to 0.7 ns, from 0.7 ns to 3.7 ns, and from 9.7 ns to 32 ns, respectively. In the same manner, a comparison for m-β-CD and γ-CD at 10 mM revealed no dependence on the fluorescence lifetime.Regarding the concentration effect of each CD variety,Φion for α-CD and γ-CD decreased by about 20% as the concentration increased. This is considered to be due to the increased difficulty of receiving the solvation stabilization energy.However, in the case of m-β-CD, in addition to τ1 becoming longer (from 0.5 ns to 1.0 ns), Φion also increased from 0.6% to 1.1%. This increase is partially because the amount of DE inclusion in CD increases at high concentrations, but also because when the DE is included on the maltose side (Figure 6), or if the DE and CD form a 1 : 2 complex, DE isomerization immediately after irradiation is blocked. This suppresses the photochromic reaction transforming the open form to closed form, and, as a result, the average lifetime becomes longer and TPI occurs more readily.Figure 6
Minimum-energy structure of the DE/m-β-CD complex calculated by MM2.


## 4. Conclusion

In the present research, we used aqueous solutions of DE in H2O/ACN (9 : 1) in the presence of α-CD, β-CD, m-β-CD, and γ-CD and calculated the respective values of Φion upon irradiation with laser light with a wavelength of 266 nm. Subsequently, we conducted a comparative examination of TPI observations and the CD concentration effect. In addition, since it is known that the fluorescence lifetime (τ) depends on Φion, we conducted a comparative investigation of this relation as well. In this regard, TPI of DE was observed upon irradiation with 266 nm light in the presence of all varieties of CD. Among them, the TPI of DE in the presence of m-β-CD suppressed the photochromic reaction by increasing the concentration of CD, resulting in a longer τ1 and an increased value of Φion. Consequently, we determined that the addition of 50 mM m-β-CD is useful with regard to Φion in the presence of CD as well as that the photochromic reaction is affected by the concentration effect of CD, which results in differences in the values of Φion.



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