# A Multimode Responsive Aptasensor for Adenosine Detection

Authors: Na Zhao; Dan Zhao; Li-ping Xu; Linfeng Chen; Yongqiang Wen; Xueji Zhang
Journal: Journal of Nanomaterials
 (2014)

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## Abstract

We report a novel multimode detection aptasensor with three signal responses (i.e., fluorescence recovery, enhanced Raman signal, and color change). The presence of adenosine induces the conformational switch of the adenosine aptamer (Apt), forming adenosine-aptamer complex and releasing quantum dots (QDs) from AuNPs, resulting in the recovered fluorescence, the enhanced Raman signal, and color change of the solution. The multimode signal recognition is potentially advantageous in improving the precision and reliability of the detection in complex environments compared to the conventional single-mode sensing system. The multimode detection strategy opens up a new possibility in sensing and quantifying more other target molecules.


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## Body

## 1. Introduction

As an endogenous purine nucleoside occurring in all cells of the human system, adenosine performs extremely important signaling functions in the cardiovascular, nervous, and immune systems. Therefore, the monitoring of adenosine is of great importance in disease diagnosis and clinical medicine [1]. Current approaches including capillary electrophoresis (CE) [2], high-performance liquid chromatography (HPLC) [3], fluorescence resonance-energy transfer (FRET) [4–7], and colorimetry [8, 9] have been demonstrated for quantitative detection of adenosine. For example, assays based on FRET using an adenosine-aptamer (Apt) modified with a fluorophore (donor) and a quencher (acceptor) allow the sensing of adenosine under intracellular or extracellular conditions [4, 6]. Liu and Lu employed a single-strand linker DNA that contained the aptamer sequence to induce the aggregation of AuNPs; the addition of adenosine dissembled the aggregated AuNPs and resulted in a purple-to-red color change [10]. Although these assays have successfully detected adenosine, one of their disadvantages is that only one kind of phenomenon or signal was applied to determine the presence of adenosine. These single-mode techniques are susceptible to the complex environments, which might be interfered in the detection process, even resulting in false results. To develop a multicomponent sensor that can yield diverse signals in response to an analyte in one pot still remains a challenging task.Over the past 20 years, DNA molecules have been utilized to design a variety of nanoscale structures and devices for a variety of applications due to the unique molecular recognition capabilities and structural features [11–13]. In parallel, not only do they function as precise assembly of specific nanostructure, but also specific DNA sequence can do catalytic functions like protein enzymes (DNAzymes) and specific binding functions like antibodies (i.e., aptamers). Aptamers, which have highly specific molecular recognition merits, have played an important role in aptamer-based biosensors of molecular diagnostics and therapeutics [14, 15]. The development of aptamers opens a new field for highly sensitive and simple detection of small molecules due to the unprecedented advantages of aptamers such as high affinity and specificity to a wide range of target molecules, including ions, small organic molecules, proteins, and cells [15–17].Taking advantage of the highly specific binding abilities of aptamers, the characteristics of flexible designability, and the highly precise positioning and control capability of DNA molecules, we herein report a multimode sensing system that can give three signals in one pot in response to adenosine simultaneously, namely, fluorescence recovery, enhanced Raman signal, and color change of the solution. Normally, the triple-mode recognition to one target has the potential to make the experiment results more reliable compared to single-response biosensors. This fundamental concept of multimode signal recognition opens up more opportunities for designing more reliable sensors systems in nanomedicine.


## 2. Materials and Methods

### 2.1. Materials

HAuCl4, trisodium citrate, cadmium oxide, zinc oxide selenium, sulfur, trioctylphosphine oxide (TOPO), tributylphosphine (TBP), 1-octadecene (ODE), oleic acid (OA), 3-mercaptopropionic acid (MPA), N-(3-dimethylaminopropyl)-N-ethylcarbodiimide (EDC), N-hydroxysuccinimide (NHS), and 4-aminothiophenol (PATP) were purchased from Sigma-Aldrich (USA). Adenosine was obtained from Solarbio (Beijing, China). CdSe QDs and AuNPs were synthesized by us according to the literatures [18, 19]. The oligonucleotides with the following sequences 5′-TGGAAGGAGG-CGTTATGAGGGGGTCCAGTCAC-C6-NH2-3′ and 5′-SH-C6-GT-GACTGGACCC-3′ were obtained and were HPLC-purified at Sangon Biotechnology Inc. (Shanghai, China). All other materials were of analytical grade and were used as received. All buffers performed with ultrapure Milli-Q water (resistance >18 MΩ·cm−1) were used in all experiments.


### 2.2. Apparatus

The morphology and the size of AuNPs were obtained by performing transmission electron microscopy (TEM, JEM-2100F). UV-Vis absorption spectra of all samples at every step of the experiments were measured with a UV-1800 UV-Vis spectrometer, in the 500 and 700 nm wavelength range. The fluorescence emission spectra were measured on a F-4500 luminescence spectrometer equipped with a xenon lamp as a light source. Two cuvettes of 2 mm path length quartz were used to measure the fluorescence and absorption spectra separately. Raman measurements were conducted in inVia-Reflex (UK) Raman spectrometer at an excitation laser of 633 nm. All experiments were carried out at room temperature under ambient conditions.


### 2.3. Preparation of Single-Stranded DNA (ssDNA) and Raman Reporter Functionalized AuNPs (Scheme1, I)

Scheme 1
Schematic representation of the multimode aptasensor for adenosine detection.AuNPs, 13 nm in diameter, were synthesized by classical Turkevich-Frens method [19]. Briefly, 100 mL H2O and 2 mL 1% HAuCl4 were added into 250 mL three-neck flask and heated under vigorous agitation. Then, 8 mL freshly prepared 1% sodium citrate solution was rapidly added to the boiling HAuCl4 solution. After 30 minutes of refluxing, the solution was cooled to room temperature. The concentration was estimated by Beer-Lambert law, based on an extinction coefficient of 2.43 × 108 M−1 cm−1 at λ = 520 nm [6]. The as-prepared stabilized 13 nm AuNPs were stored at 4°C. For ssDNA-AuNPs, thiolated DNA oligonucleotides (5′-SH-C6-GTGACTGGA-CCC-3′, 70 μL, 10 μM) were added to 200 μL AuNPs (16 nM) aqueous solution. After overnight incubation, the Raman reporters (PATP ethanol solutions, 10 μL, 0.03 μM) were added into as-prepared AuNPs solutions for 24 h. After that, the AuNPs conjugates labeled by ssDNA and Raman reporters were “aged” in 0.1 M PBS solution (pH 7.4) for 24 h at ambient temperature [4]. Unconjugated oligonucleotides were removed by centrifugation at 12000 rpm for 15 min and the red oily precipitate was washed, recentrifuged, and then dispersed in 200 μL Tris-HCl (20 mM, pH 7.4) at 4°C for future use.


### 2.4. Preparation of Apt Functionalized CdSe/ZnS QDs (Scheme1, II)

CdSe/ZnS QDs were synthesized by the well-established method with MPA modification [18]. The concentration of CdSe/ZnS QDs wascalculated by classical Xiaogang Peng method [20]. The COOH-functionalized QDs (0.04 nmol) were activated with 60 nmol EDC and 60 nmol NHS for 30 min, followed by incubating with Apt (5′-TG-GAAGGAGGCGTTATGAGGGGGTCCAGTCAC-C6-NH2-3′) for 12 h. After that, aqueous NaCl (40 μL, 1 M) and CH3CH2OH (20 μL) were added; the mixture was then purified in a centrifuge at 8000 rpm for 5 min. The Apt-ODs were then dispersed in Tris-HCl buffer (13.6 μL, 20 mM, pH 7.4) [6].


### 2.5. Preparation of QDs-AuNPs Probes (Scheme1, III) and Adenosine Detection

In order to ensure the stability and accuracy of the experiment result, the DNA strands on Apt-QDs (Scheme1, II) and ssDNA-AuNPs (Scheme 1, I) conjugates should form double-strand DNA conjugate as much as possible. We mixed Apt-QDs and ssDNA-AuNPs conjugates in a series of molar ratio and incubated at 70°C for 10 min, and then cooled down to room temperature to ensure the maximization of hybridization. Fluorescence quenching efficiency was used to detect the hybridization efficiency of the two DNA strands. By performing a series of experiments, the optimum molar ratio of Apt-QDs and ssDNA-AuNPs conjugates was determined as 12 : 1. Then, the QDs-AuNPs probes solution was centrifuged and washed once with an equal volume of PBS (pH 7.4) and finally resuspended in PBS (1 mL, pH 7.4). For analyte detection, adenosine of various concentrations was added to the QDs-AuNPs probes solution and incubated for 30 min at room temperature.


## 2.1. Materials

HAuCl4, trisodium citrate, cadmium oxide, zinc oxide selenium, sulfur, trioctylphosphine oxide (TOPO), tributylphosphine (TBP), 1-octadecene (ODE), oleic acid (OA), 3-mercaptopropionic acid (MPA), N-(3-dimethylaminopropyl)-N-ethylcarbodiimide (EDC), N-hydroxysuccinimide (NHS), and 4-aminothiophenol (PATP) were purchased from Sigma-Aldrich (USA). Adenosine was obtained from Solarbio (Beijing, China). CdSe QDs and AuNPs were synthesized by us according to the literatures [18, 19]. The oligonucleotides with the following sequences 5′-TGGAAGGAGG-CGTTATGAGGGGGTCCAGTCAC-C6-NH2-3′ and 5′-SH-C6-GT-GACTGGACCC-3′ were obtained and were HPLC-purified at Sangon Biotechnology Inc. (Shanghai, China). All other materials were of analytical grade and were used as received. All buffers performed with ultrapure Milli-Q water (resistance >18 MΩ·cm−1) were used in all experiments.


## 2.2. Apparatus

The morphology and the size of AuNPs were obtained by performing transmission electron microscopy (TEM, JEM-2100F). UV-Vis absorption spectra of all samples at every step of the experiments were measured with a UV-1800 UV-Vis spectrometer, in the 500 and 700 nm wavelength range. The fluorescence emission spectra were measured on a F-4500 luminescence spectrometer equipped with a xenon lamp as a light source. Two cuvettes of 2 mm path length quartz were used to measure the fluorescence and absorption spectra separately. Raman measurements were conducted in inVia-Reflex (UK) Raman spectrometer at an excitation laser of 633 nm. All experiments were carried out at room temperature under ambient conditions.


## 2.3. Preparation of Single-Stranded DNA (ssDNA) and Raman Reporter Functionalized AuNPs (Scheme1, I)

Scheme 1
Schematic representation of the multimode aptasensor for adenosine detection.AuNPs, 13 nm in diameter, were synthesized by classical Turkevich-Frens method [19]. Briefly, 100 mL H2O and 2 mL 1% HAuCl4 were added into 250 mL three-neck flask and heated under vigorous agitation. Then, 8 mL freshly prepared 1% sodium citrate solution was rapidly added to the boiling HAuCl4 solution. After 30 minutes of refluxing, the solution was cooled to room temperature. The concentration was estimated by Beer-Lambert law, based on an extinction coefficient of 2.43 × 108 M−1 cm−1 at λ = 520 nm [6]. The as-prepared stabilized 13 nm AuNPs were stored at 4°C. For ssDNA-AuNPs, thiolated DNA oligonucleotides (5′-SH-C6-GTGACTGGA-CCC-3′, 70 μL, 10 μM) were added to 200 μL AuNPs (16 nM) aqueous solution. After overnight incubation, the Raman reporters (PATP ethanol solutions, 10 μL, 0.03 μM) were added into as-prepared AuNPs solutions for 24 h. After that, the AuNPs conjugates labeled by ssDNA and Raman reporters were “aged” in 0.1 M PBS solution (pH 7.4) for 24 h at ambient temperature [4]. Unconjugated oligonucleotides were removed by centrifugation at 12000 rpm for 15 min and the red oily precipitate was washed, recentrifuged, and then dispersed in 200 μL Tris-HCl (20 mM, pH 7.4) at 4°C for future use.


## 2.4. Preparation of Apt Functionalized CdSe/ZnS QDs (Scheme1, II)

CdSe/ZnS QDs were synthesized by the well-established method with MPA modification [18]. The concentration of CdSe/ZnS QDs wascalculated by classical Xiaogang Peng method [20]. The COOH-functionalized QDs (0.04 nmol) were activated with 60 nmol EDC and 60 nmol NHS for 30 min, followed by incubating with Apt (5′-TG-GAAGGAGGCGTTATGAGGGGGTCCAGTCAC-C6-NH2-3′) for 12 h. After that, aqueous NaCl (40 μL, 1 M) and CH3CH2OH (20 μL) were added; the mixture was then purified in a centrifuge at 8000 rpm for 5 min. The Apt-ODs were then dispersed in Tris-HCl buffer (13.6 μL, 20 mM, pH 7.4) [6].


## 2.5. Preparation of QDs-AuNPs Probes (Scheme1, III) and Adenosine Detection

In order to ensure the stability and accuracy of the experiment result, the DNA strands on Apt-QDs (Scheme1, II) and ssDNA-AuNPs (Scheme 1, I) conjugates should form double-strand DNA conjugate as much as possible. We mixed Apt-QDs and ssDNA-AuNPs conjugates in a series of molar ratio and incubated at 70°C for 10 min, and then cooled down to room temperature to ensure the maximization of hybridization. Fluorescence quenching efficiency was used to detect the hybridization efficiency of the two DNA strands. By performing a series of experiments, the optimum molar ratio of Apt-QDs and ssDNA-AuNPs conjugates was determined as 12 : 1. Then, the QDs-AuNPs probes solution was centrifuged and washed once with an equal volume of PBS (pH 7.4) and finally resuspended in PBS (1 mL, pH 7.4). For analyte detection, adenosine of various concentrations was added to the QDs-AuNPs probes solution and incubated for 30 min at room temperature.


## 3. Results and Discussion

The principle of FERT biosensor is shown in Scheme1. In the first step, the AuNPs were modified with the thiol-DNA, which could be easily absorbed onto citrate coated AuNPs and stabilize the AuNPs in salt or buffer solution. Then the ssDNA-attached AuNPs (ssDNA-AuNPs), in which the DNA sequence was partially complementary with the Apt sequence, were labeled with Raman reporter through the Au-S bonds. The process was shown in Scheme 1a. Subsequently, the AuNPs conjugates (Scheme 1, I) were hybridized with the Apt-QDs (Scheme 1, II), forming the QDs-AuNPs probes (Scheme 1, III) with red in high salt concentration (Scheme 1b). In this conformation, the nanoprobe only exhibited small background fluorescence due to the fluorescence transferring from the QDs to the AuNPs when they are in close proximity.FRET between QDs as donor and AuNPs as acceptor was used for detection of adenosine. As an alternative developed inorganic fluorescent material, QDs have unique virtues compared to the traditional fluorophores, such as size tunable photoluminescence, high quantum yields, narrow-size tunable spectra, and low photobleaching [21, 22]. Gold nanoparticles have been of great interest because of the superior quenching effect and the color change associated with its states. Thiolated molecules (such as ssDNA and Raman reporter) especially can be easily attached on the AuNPs surface through strong gold-sulphur covalent reaction [23].When the adenosine is present, the aptamer-target binding displaced the Watson-Crick binding between the Apt and the linker DNA sequence on QDs, which induces the conformational switch of the Apt from loose random coil to a G-quadruplex structure, which disassembles the original duplex and the QDs were separated from the AuNPs, affording fluorescence “turn-on” state due to the far distance between donor and acceptor (Scheme1c). The relationship between the increase of fluorescence intensity and the adenosine concentration provides the basis for the quantitation of adenosine. Simultaneously, the ssDNA-AuNPs were aggregated (Scheme 1, IV) because of the different stabilities of ssDNA-AuNPs and double-stranded (ds) DNA-attached AuNPs (dsDNA-AuNPs) in a high salt concentration, leading to a corresponding color change from red to purple (Scheme 1c). This process leads to redistribution of the ssDNA-AuNPs and a great enhancement of the EM field at a specific position around the AuNPs surface (called “hot spots”). The “hot spots” were formed between the junctions of the AuNPs aggregates (Scheme 1, IV), giving rise to enhanced Raman scattering signal under the light excitation at wavelength of 633 nm (Scheme 1c).


### 3.1. Fluorescence Quenching Efficiency by the AuNPs

The fluorescence of the QDs in the QDs-AuNPs probes was highly quenched by the AuNPs through the FRET. Because the quenching efficiency within the conjugates is one of the key factors for the sensitivity of adenosine detection, we first determined the quenching efficiency of our system by comparing the fluorescence signal of both the QDs and QDs-AuNPs probes. Fluorescence spectra of QDs donor in the absence and the presence of the acceptor AuNPs were shown in Figure1, respectively. Quenching efficiency was calculated by [24]
(1)
Quenching
efficiency
=
F
0
-
F
F
0
.
In (1), F
0, F are the fluorescence intensity of QDs at 606 nm before and after addition of ssDNA-AuNPs conjugates, respectively. Maximal quenching efficiency 75% was observed in Figure 1 with 12 : 1 molar ratio of Apt-QDs to ssDNA-AuNPs. The result suggests that FRET indeed occurred and ssDNA-AuNPs are highly effective to quench the QDs fluorescence.Figure 1
Fluorescence spectra of QDs in the absence and the presence of the AuNPs.


### 3.2. Effect of the Ionic Strength to the QDs-AuNPs and ssDNA-AuNPs Systems

AuNPs have been widely applied as colorimetric reporter in biomolecular detection [25–27]. The change in color of the dispersed AuNPs (red) and aggregated AuNPs (purple) can be easily read with the naked eye. Li and Rothberg found that ss-DNA and ds-DNA have different binding affinities to negatively charged AuNPs, thus resulting in the different stabilities of ssDNA-AuNPs and dsDNA-AuNPs in a high salt concentration [28].Based on this principle, several colorimetric assays have been developed. For example, Sato and coworkers demonstrated a colorimetric detection of DNA by using the different stabilities of ssDNA-AuNPs before and after the hybridization with the complementary ssDNA. Surprisingly, they found that fully matched dsDNA-AuNPs had a lower stability than ssDNA-AuNPs [29]. On the basis of these studies, Zhao and coworkers found that dsDNA-AuNPs, which are not completely paired, were much more stable than ssDNA-AuNPs [30]. In order to determine the stabilities of ssDNA-AuNPs and QDs-AuNPs, following experiments were performed to detect the different salt tolerance of the two systems.At a situation of 100 mM NaCl (pH = 7.4), a different amount of MgCl2 was slowly added into the ssDNA-AuNPs and QDs-AuNPs solutions independently. As shown in Figure 2, when the concentration of MgCl2 of the two systems was 3 mM and 8 mM, respectively, the UV absorption redshifts significantly. The redshift of the UV absorption was induced by the aggregation of AuNPs, which confirmed the fact that partially complementary dsDNA-AuNPs possessed higher salt tolerance than ssDNA-AuNPs. In subsequent experiments, the reaction systems were maintained 4 mM MgCl2. At that situation QDs-AuNPs systems were stable whereas ssDNA-AuNPs were not.UV-Vis spectra of ssDNA-AuNPs and QDs-AuNPs solutions in the presence of MgCl2 at different concentrations. The concentrations of MgCl2 were 0, 1, 2, 2.5, and 3 mM for ssDNA-AuNPs probes (a) and 0, 2, 4, 6, and 8 mM for QDs-AuNPs probes (b). Inset: the plot of the wavelength ratio (λ
/
λ
535) versus the increasing concentrations of MgCl2 of the same data, respectively.
(a)
(b)


### 3.3. Adenosine Substance Detection

Surface-enhanced Raman scattering (SERS) is an ultrasensitive vibrational spectroscopic technique that has long been an alternative tool for biosensing because of its unique features, such as hyperfine resolution ability, noninvasive advantage, and relaxed testing conditions [31–33]. It is acknowledged that a remarkable surface enhancement Raman scattering effect can be generated at the nanoscale junctions and interstices in interacting metal nanostructures such as nanoparticles dimmers or aggregates. Usually, an enhanced signal detected at the junctions between nanoparticles of the aggregates is 2–40 times stronger than that obtained at an isolated nanoparticle due to the generation of the “hot spots” [34]. In our work, the “hot spots” were formed between the aggregated AuNPs after the addition of adenosine, leading to the SERS signal enhancement.Under optimized conditions, the analytical performance of the multimode aptasensor for adenosine detection was investigated. In the QDs-AuNPs conformation, the fluorescence was efficiently quenched by AuNPs due to close donor and acceptor distance. Upon the addition of adenosine, the constrained conformation was turned on in response to the adenosine binding with its aptamer; the QDs were separated from the AuNPs, which led to a significant fluorescence increase in the emission at 606 nm. This adenosine binding process resulted in the aggregation of the ssDNA-AuNPs in high salt solution with concomitant red-to-purple color change and enhanced Raman scattering signal. Figure3(a) shows the fluorescence spectra of the QDs-AuNPs probes after adding various concentrations of adenosine. Sequential increases in the fluorescence emission spectrum of QDs were observed when fixed volumes of QDs-AuNPs conjugates (200 μL) were incubated with increasing adenosine concentrations. As shown, the fluorescence intensity was proportionally increased according to the concentrations of adenosine in a range of 0 to 60 μM. Furthermore, a linear range of the fluorescence response versus the F
/
F
0 of the same data was also shown in Figure 3(a). The peaks at 1081 and 1583 cm−1 of the Raman spectra were the characteristics of PATP molecules, which was observed upon the addition of adenosine at various concentrations in Figure 3(c). As shown in Figures 3(c) and 3(d), the intensity of the Raman peaks increased with the increase of the adenosine concentrations. The SERS response became saturated when the adenosine concentration reached 70 μM (data not shown). Therefore, the range of the SERS response was 10 μM to 60 μM as shown in Figure 3(c). On the contrary, no evident PATP Raman peak associated with AuNPs was observed in the absence of adenosine, which was consistent with the result in Figure 3(c)(A). Correspondingly, the color change of the solution further confirmed the aggregation of the AuNPs, as shown in Figure 3(b). Thus, three signals were observed simultaneously within 30 minutes upon the addition of the target molecules. The general concept of multimode detection in one pot will be of significant importance in improving reliability of the analysis.(a) Fluorescence spectra depending on adenosine concentrations from 0 to 60μM. (Inset: the plot of the fluorescence ratio (
F
/
F
0
) versus the increasing concentrations of adenosine of the same data.) (b) Photograph showing colorimetric responses of detection system in the presence of 0 μM and 40 μM adenosine. (c) Raman spectra obtained from the QDs-AuNPs probe with various concentrations of adenosine. (d) Raman intensity at peak of 1081 cm−1 and 1583 cm−1 of the QDs-AuNPs probe incubation with different concentrations of adenosine.
(a)
(b)
(c)
(d)


## 3.1. Fluorescence Quenching Efficiency by the AuNPs

The fluorescence of the QDs in the QDs-AuNPs probes was highly quenched by the AuNPs through the FRET. Because the quenching efficiency within the conjugates is one of the key factors for the sensitivity of adenosine detection, we first determined the quenching efficiency of our system by comparing the fluorescence signal of both the QDs and QDs-AuNPs probes. Fluorescence spectra of QDs donor in the absence and the presence of the acceptor AuNPs were shown in Figure1, respectively. Quenching efficiency was calculated by [24]
(1)
Quenching
efficiency
=
F
0
-
F
F
0
.
In (1), F
0, F are the fluorescence intensity of QDs at 606 nm before and after addition of ssDNA-AuNPs conjugates, respectively. Maximal quenching efficiency 75% was observed in Figure 1 with 12 : 1 molar ratio of Apt-QDs to ssDNA-AuNPs. The result suggests that FRET indeed occurred and ssDNA-AuNPs are highly effective to quench the QDs fluorescence.Figure 1
Fluorescence spectra of QDs in the absence and the presence of the AuNPs.


## 3.2. Effect of the Ionic Strength to the QDs-AuNPs and ssDNA-AuNPs Systems

AuNPs have been widely applied as colorimetric reporter in biomolecular detection [25–27]. The change in color of the dispersed AuNPs (red) and aggregated AuNPs (purple) can be easily read with the naked eye. Li and Rothberg found that ss-DNA and ds-DNA have different binding affinities to negatively charged AuNPs, thus resulting in the different stabilities of ssDNA-AuNPs and dsDNA-AuNPs in a high salt concentration [28].Based on this principle, several colorimetric assays have been developed. For example, Sato and coworkers demonstrated a colorimetric detection of DNA by using the different stabilities of ssDNA-AuNPs before and after the hybridization with the complementary ssDNA. Surprisingly, they found that fully matched dsDNA-AuNPs had a lower stability than ssDNA-AuNPs [29]. On the basis of these studies, Zhao and coworkers found that dsDNA-AuNPs, which are not completely paired, were much more stable than ssDNA-AuNPs [30]. In order to determine the stabilities of ssDNA-AuNPs and QDs-AuNPs, following experiments were performed to detect the different salt tolerance of the two systems.At a situation of 100 mM NaCl (pH = 7.4), a different amount of MgCl2 was slowly added into the ssDNA-AuNPs and QDs-AuNPs solutions independently. As shown in Figure 2, when the concentration of MgCl2 of the two systems was 3 mM and 8 mM, respectively, the UV absorption redshifts significantly. The redshift of the UV absorption was induced by the aggregation of AuNPs, which confirmed the fact that partially complementary dsDNA-AuNPs possessed higher salt tolerance than ssDNA-AuNPs. In subsequent experiments, the reaction systems were maintained 4 mM MgCl2. At that situation QDs-AuNPs systems were stable whereas ssDNA-AuNPs were not.UV-Vis spectra of ssDNA-AuNPs and QDs-AuNPs solutions in the presence of MgCl2 at different concentrations. The concentrations of MgCl2 were 0, 1, 2, 2.5, and 3 mM for ssDNA-AuNPs probes (a) and 0, 2, 4, 6, and 8 mM for QDs-AuNPs probes (b). Inset: the plot of the wavelength ratio (λ
/
λ
535) versus the increasing concentrations of MgCl2 of the same data, respectively.
(a)
(b)


## 3.3. Adenosine Substance Detection

Surface-enhanced Raman scattering (SERS) is an ultrasensitive vibrational spectroscopic technique that has long been an alternative tool for biosensing because of its unique features, such as hyperfine resolution ability, noninvasive advantage, and relaxed testing conditions [31–33]. It is acknowledged that a remarkable surface enhancement Raman scattering effect can be generated at the nanoscale junctions and interstices in interacting metal nanostructures such as nanoparticles dimmers or aggregates. Usually, an enhanced signal detected at the junctions between nanoparticles of the aggregates is 2–40 times stronger than that obtained at an isolated nanoparticle due to the generation of the “hot spots” [34]. In our work, the “hot spots” were formed between the aggregated AuNPs after the addition of adenosine, leading to the SERS signal enhancement.Under optimized conditions, the analytical performance of the multimode aptasensor for adenosine detection was investigated. In the QDs-AuNPs conformation, the fluorescence was efficiently quenched by AuNPs due to close donor and acceptor distance. Upon the addition of adenosine, the constrained conformation was turned on in response to the adenosine binding with its aptamer; the QDs were separated from the AuNPs, which led to a significant fluorescence increase in the emission at 606 nm. This adenosine binding process resulted in the aggregation of the ssDNA-AuNPs in high salt solution with concomitant red-to-purple color change and enhanced Raman scattering signal. Figure3(a) shows the fluorescence spectra of the QDs-AuNPs probes after adding various concentrations of adenosine. Sequential increases in the fluorescence emission spectrum of QDs were observed when fixed volumes of QDs-AuNPs conjugates (200 μL) were incubated with increasing adenosine concentrations. As shown, the fluorescence intensity was proportionally increased according to the concentrations of adenosine in a range of 0 to 60 μM. Furthermore, a linear range of the fluorescence response versus the F
/
F
0 of the same data was also shown in Figure 3(a). The peaks at 1081 and 1583 cm−1 of the Raman spectra were the characteristics of PATP molecules, which was observed upon the addition of adenosine at various concentrations in Figure 3(c). As shown in Figures 3(c) and 3(d), the intensity of the Raman peaks increased with the increase of the adenosine concentrations. The SERS response became saturated when the adenosine concentration reached 70 μM (data not shown). Therefore, the range of the SERS response was 10 μM to 60 μM as shown in Figure 3(c). On the contrary, no evident PATP Raman peak associated with AuNPs was observed in the absence of adenosine, which was consistent with the result in Figure 3(c)(A). Correspondingly, the color change of the solution further confirmed the aggregation of the AuNPs, as shown in Figure 3(b). Thus, three signals were observed simultaneously within 30 minutes upon the addition of the target molecules. The general concept of multimode detection in one pot will be of significant importance in improving reliability of the analysis.(a) Fluorescence spectra depending on adenosine concentrations from 0 to 60μM. (Inset: the plot of the fluorescence ratio (
F
/
F
0
) versus the increasing concentrations of adenosine of the same data.) (b) Photograph showing colorimetric responses of detection system in the presence of 0 μM and 40 μM adenosine. (c) Raman spectra obtained from the QDs-AuNPs probe with various concentrations of adenosine. (d) Raman intensity at peak of 1081 cm−1 and 1583 cm−1 of the QDs-AuNPs probe incubation with different concentrations of adenosine.
(a)
(b)
(c)
(d)


## 4. Conclusions

In summary, taking advantage of the highly specific binding abilities of aptamers, conformational flexibility of the DNA constituents, we established a system that can give three signal responses to adenosine in one pot. The design relies on the structure-switching properties of aptamers upon binding to their target molecules. Using Apt-QDs as the molecular recognition element and the Raman molecule labeled ssDNA-AuNPs as the signaling probe, this triple-mode response aptasensor reported here opens up a new possibility of rapid, easy, and reliable detection of adenosine from more complex environments. Furthermore, our strategy provides a feasible platform for the detection of more other biomolecules owing to the high affinity and specificity of aptamers to a wide range of targets molecules. The innovative approach also holds promising potential for broad applications in the environmental monitoring, food safety, and clinical applications. Further detailed quantitative research is worth being carried out.



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*Source: 360347-2014-12-21.xml*