# Anti-Inflammatory Activity of Babassu Oil and Development of a Microemulsion System for Topical Delivery

Authors: Mysrayn Y. F. A. Reis; Simone M. dos Santos; Danielle R. Silva; Márcia V. Silva; Maria Tereza S. Correia; Daniela M. A. Ferraz Navarro; Geanne K. N. Santos; Fernando Hallwass; Otávio Bianchi; Alexandre G. Silva; Janaína V. Melo; Alessandra B. Mattos; Rafael M. Ximenes; Giovanna Machado; Karina L. A. Saraiva
Journal: Evidence-Based Complementary and Alternative Medicine
 (2017)

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## Abstract

Babassu oil extraction is the main income source in nut breakers communities in northeast of Brazil. Among these communities, babassu oil is used for cooking but also medically to treat skin wounds and inflammation, and vulvovaginitis. This study aimed to evaluate the anti-inflammatory activity of babassu oil and develop a microemulsion system with babassu oil for topical delivery. Topical anti-inflammatory activity was evaluated in mice ear edema using PMA, arachidonic acid, ethyl phenylpropiolate, phenol, and capsaicin as phlogistic agents. A microemulsion system was successfully developed using a Span® 80/Kolliphor® EL ratio of 6 : 4 as the surfactant system (S), propylene glycol and water (3 : 1) as the aqueous phase (A), and babassu oil as the oil phase (O), and analyzed through conductivity, SAXS, DSC, TEM, and rheological assays. Babassu oil and lauric acid showed anti-inflammatory activity in mice ear edema, through inhibition of eicosanoid pathway and bioactive amines. The developed formulation (39% A, 12.2% O, and 48.8% S) was classified as a bicontinuous to o/w transition microemulsion that showed a Newtonian profile. The topical anti-inflammatory activity of microemulsified babassu oil was markedly increased. A new delivery system of babassu microemulsion droplet clusters was designed to enhance the therapeutic efficacy of vegetable oil.


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## Body

## 1. Introduction

Babassu palm tree (Attalea speciosa Mart. ex Spreng, Arecaceae) is commonly found in North and Northeast regions of Brazil, mainly in the phytogeographic domain of“Mata dos Cocais” (Port. Lit. Palm trees forest). In its areas of occurrence, it represents the main income source for about 300.000 people in nut breakers communities in northeast region of Brazil, most of them in state of Maranhão, but also in Piauí and in Chapada do Araripe/CE. Gathering of fruits to produce oil or to sell the nuts is activities predominantly made by women, who are called“quebradeiras de coco babaçu” (Por. Lit. Bassabu nut breaker women) [1–3]. These communities developed autochthonous knowledge about babassu uses as source of human and animal food, utensils and tools, fuel, construction, and soil fertilizers and as cosmetic and medicines [3–6].Among the parts used for medicinal purposes, leaves, roots, and fruits should be highlighted. Leaves and roots are used as tea for pain and wound healing, while fruits are used in a much bigger scale: mesocarp floor and milk are used for the treatment of gastritis, hepatitis, osteoporosis, skin wounds, and leukorrhea; liquid albumen is used as eyedrops to treat conjunctivitis; and the seed oil is used as laxative, vermifuge, and anti-inflammatory and for the treatment of myiasis, mycosis, skin wounds, hemorrhoids, leukorrhea and female genital inflammation, and spider bites [3–5, 7].Vegetable oils such as olive, palm, and coconut oils are well known for their anti-inflammatory properties. Olive oil is rich in anti-inflammatory phenolics like oleocanthal and oleuropein glycosides [8, 9], while the effect of palm oil is attributed to its high tocotrienol content [10]. Like babassu oil, coconut oil is poor in phenolics and tocopherols. Its anti-inflammatory effect is assigned to the presence of lauric acid and glyceryl laurates [11].The availability of babassu oil and its easy handling have enabled development of several types of formulations to enhance the therapeutic efficacy of the biologically active components. Targeted delivery can be achieved by applying pharmaceutical nanotechnology, which is based on the synthesis, application, and characterization of nanoscale therapeutic systems to provide a more controlled drug release. In this context, nanostructured systems, for example, microemulsions containing babassu oil, may act as new and potentially efficient therapies for benign prostatic hyperplasia due to their antiproliferative and apoptotic effects [12] and improve human immune system function by increasing superoxide anion release, phagocytosis of mononuclear phagocytes, and antimicrobial activities [13].Microemulsion is a system of two immiscible fluids that is stabilized by an interfacial film of surfactants. It offers the advantages of spontaneous formation, thermodynamic stability, manufacturing simplicity, solubilization capacity of lipophilic and/or hydrophilic solutes, a large area per volume ratio for mass transfer, and the potential for permeation enhancement [14–17]. Thus, the aim of this work was to evaluate the topical anti-inflammatory activity of babassu oil obtained from Chapada do Araripe in Ceará (Brazil) and synthesize and characterize a microemulsion system aiming to enhance babassu oil topical anti-inflammatory activity.


## 2. Material and Methods

### 2.1. Chemicals

Phorbol 12-myristate 13-acetate (PMA), arachidonic acid (AA), ethyl phenylpropiolate (EPP), phenol, and capsaicin were purchased from Sigma Chemical Co. (St. Louis, MO, USA). The surfactants, Span 80 (sorbitan mono-oleate) and Tween® 80 (polyoxyethylene sorbitan monooleate), ethanol, 99.9% deuterated CDCl3, and Supelco® 37 Component FAME Mix were also obtained from Sigma Chemical Co. Kolliphor EL (polyoxyl castor oil) was from BASF SE (Ludwigshafen, Germany) and propylene glycol PA was purchased from Neon Comercial Ltda (São Paulo, Brazil).


### 2.2. Plant Material and Oil Extraction

Babassu fruits were collected from one palm tree in Araripe, located in the State of Ceará (Brazil), in July 2013. Botanical authentication was conducted by Olívia O. Cano, and a voucher specimen was deposited at the Herbarium of the Agronomic Institute of Pernambuco under number 90,472.The oil was extracted by a well-established method that is practiced by farmers in forested areas. After harvesting, the seeds were ground with a stone grinder, and the obtained paste was mixed well and allowed to stand overnight. On the next day, cold water was added to the paste, and the upper part was collected and placed on a fire to heat it until it started boiling. Subsequently, the liquid phase was separated from the oil meal using a tissue and heated until the water completely evaporated. Finally, the oil was filtered and used [18].


### 2.3. Physicochemical Characterization and Fatty Acid Profile

Traditionally extracted babassu oil was immediately analysed for some physicochemical properties as described by Adolfo Lutz Institute [19] as follows: relative density was determined in a 10 mL glass hydrometer (pycnometer) at 25°C; refractive index at 40°C was performed using a Abbé refractometer; peroxide values were calculated from the iodine release from potassium iodide and expressed as milliequivalents of active oxygen/kg of oil; rancidity (lipid oxidation) was determined by Kreis reaction using phloroglucinol in acid medium; acid values were expressed as milligrams of KOH required to neutralize the free acids of 1 g of oil, which was determined by titration in methanolic solution.The transesterification procedure of the oil was realized in accord with Metcalfe et al. [20]. The oil (150 mg) was mixed with 4.0 mL of NaOH in 0.50 M methanol and incubated at 100°C for 5 minutes. Next, 5.0 mL solution of BF3 (12%) in methanol was added in the mixture and was heated for 2 minutes. After cooling to room temperature, 5.0 mL of a saturated sodium chloride solution was added. The mixture was transferred to a separation funnel containing 20.0 mL of petroleum ether and vigorously stirred. After a period of rest, the aqueous phase was discarded and the ether phase was filtered. The solvent was evaporated in a rotary evaporator at 60°C and the residual solvent was removed with nitrogen flow. Methyl esters were solubilized in n-heptane before injection into the gas chromatographer [21].The samples were analyzed using an Agilent Technologies (Palo Alto, CA, USA) 5975C single quadrupole GC-MS equipped with a nonpolar HP-5MS (Agilent) fused silica capillary column (30 m × 0.25 mm i.d.; film thickness 0.25 mm). The oven was initially held at 150°C for 2 min, increased to 230°C by 5°C/min (held for 7 min), and finally increased to 260°C by 4°C/min. The final temperature was maintained for 7.5 min. The carrier gas was helium supplied at a constant flow of 1 mL/min and the split/splitless injector was maintained at 230°C. The applied ionization potential was 70 eV; the scan range was from 35 to 450m/z with scan rate of 0.5 scans/s. A standard fatty acid methyl ester mixture (Supelco 37 Component FAME Mix, USA) was used to identify the fatty acid methyl esters. Fatty acid data were expressed as percentage of total peak area.


### 2.4. NMR Analysis

The extracted oil (approximately 50 mg) was dissolved in 0.6 mL of CDCl3 and placed in a 5 mm NMR tube. The NMR analyses were performed on a Varian 400-VNMRS (Agilent Technologies, CA, USA) at 26°C, operating at the frequencies of 399.74 and 100.51 MHz for 1H and 13C, respectively. 1H NMR spectra were recorded with 2.5 s acquisition times, sweep widths of 6.4 kHz, 45° pulse angles, and 1 s delay times. C13{H1} NMR spectra were recorded with 3.04 s acquisition times, sweep widths of 21.5 kHz, 45° pulse angles, and 1 s delay times. For processing 13C spectra, zero filling and line broadening of 1 Hz were applied prior to Fourier transformation. The chemical shift scale was indirectly referred to tetramethylsilane (TMS) by the residual CHCl3 signal at 7.26 ppm for 1H spectra and the signal at 77.0 ppm for 13C spectra.


### 2.5. Topical Anti-Inflammatory Activity

#### 2.5.1. Animals

Male Swiss and BALB/c mice (25–30 g,n=6) were provided by the Animal Facility of Federal University of Pernambuco. The animals were housed and kept in a room with controlled temperature (23±2°C) under a 12/12 h light/dark cycle with food and water ad libitum. Experiments were conducted according to the Guide for the Care and Use of Laboratory Animals from the US Department of Health and Human Services. The project had been previously approved by the Animal’s Ethics Committee of Federal University of Pernambuco (number 23076.039887/2014-51).


#### 2.5.2. Ear Edema Measurement

For evaluation of ear weight, mice were euthanized and 6 mm diameter samples were taken from both ears using a biopsy punch (Richter®, Brazil). Each biopsy was weighed on a semi-micro analytical balance (AUW-D 220, Shimadzu, Japan). Ear edema (EE) was expressed as the increase in ear sample weight, using the following formula:(1)EEmg=wRE-wLE,where wRE is the weight obtained from the right ear sample (inflamed ear) and wLE is the weight obtained from the left ear sample (noninflamed ear).For all treatments, animals were anesthetized with 1% halothane. Right ears were then challenged with different phlogistic agents diluted in acetone (20μL). Babassu oil (1, 3, and 10 μL/ear) was applied topically in 20 μL acetone, while microemulsion was applied with no dilution. Dexamethasone or indomethacin (0.1 and 0.5 mg/ear, resp.) was used topically as a positive control. Ruthenium red (3 mg/kg, s.c.) was used as a positive control for capsaicin-induced ear edema.


#### 2.5.3. PMA-Induced Ear Edema

Ear edema was induced by topical application of 20μL of PMA (2.5 μg/ear) in acetone on both sides of the right ear. Left ears received only acetone as a control. After 15 min, 10 μL of babassu oil (100 and 12.2%), babassu microemulsion, microemulsion vehicle (48.8% surfactants, 39% aqueous phase, and 12.2% water), 0.5% dexamethasone, or acetone were applied to both sides of the right and left ears. Six hours after PMA administration, mice were euthanized for ear edema measurement [22, 23]. To avoid misinterpretation of the results due to a possible barrier effect of babassu oil on ear surfaces, an oral treatment with babassu oil was also evaluated against PMA-induced ear edema. Shortly, mice were treated with babassu oil (100, 300, and 1000 mg/kg) or indomethacin (10 mg/kg, p.o.) 60 min before PMA administration [23].


#### 2.5.4. Investigation of Babassu Oil Mode of Action on Ear Edema

For elucidation of the mechanisms underlying the topical anti-inflammatory activity of babassu oil on PMA-induced mice ear edema, different phlogistic agents were used to induce ear edema: arachidonic acid (2 mg/ear), ethyl phenylpropiolate 5% (20μL/ear), phenol 10% (20 μL/ear), and capsaicin (0.25 mg/ear). Immediately after acetone evaporation, babassu oil (10 μL/ear) or indomethacin (0.5 mg/ear)/dexamethasone (0.1 mg/ear) were topically applied on both sides of the right ear, while the left ear received 20 μL of the vehicle. Ruthenium red (3 mg/kg, s.c.) was used as positive control for capsaicin-induced ear edema and it was administered 30 min before capsaicin.After 1 h, mice were euthanized for ear edema measurement. Mice challenged with capsaicin were euthanized after 30 min of exposure. AA-induced ear edema was performed in BALB/c mice [23–25].


### 2.6. Preparation of Emulsions

A series of oil-in-water emulsions with HLB values ranging from 4.5 to 15 was prepared with the surfactants Span 80 and Tween 80 at a 2% total blend concentration w/v, 93% water, and 5% babassu oil using the Griffin equation [26]. The aqueous phase (water plus Tween 80) and the oil phase (oil plus Span 80) were heated to 70±5°C separately. Both phases were mixed by the inversion method with mechanical stirring (9.000 rpm) for 5 min in ULTRA-TURRAX homogenizer equipment (Unique/DES500, São Paulo, Brazil). The samples were characterized 24 h after preparation.


### 2.7. Droplet Size Analysis by Dynamic Light Scattering (DLS)

The droplet size distribution of the dispersed phase of the emulsions was determined by DLS using the Nanotrac Wave equipment (Microtrac Inc., Montgomeryville, PA, USA) with measurement capability from 0.8 to 6500 nanometers. The data were calculated using the manufacturer’s software. Each emulsion was diluted with aqueous propylene glycol (1 : 100) and analyzed in triplicate.


### 2.8. Turbidimetric Method

The emulsions were diluted 1 : 25 with aqueous propylene glycol, and the percentage of transmission (%T) was measured at 600 nm using a spectrophotometer (UV-mini 1240, Shimadzu, Japan). With the blank control set at 100% transmission, the turbidity of the diluted emulsion was calculated as follows: turbidity = 100 − %T [27]. The reported results were the average of three determinations.


### 2.9. Construction of the Pseudo-Ternary Phase Diagram

Babassu oil, Span 80, and Kolliphor EL were selected as the oil phase and surfactants, respectively. Surfactant mixtures were tested at a ratio of 6 : 4 (w/w), as defined by the Griffin equation. The pseudo-ternary phase diagrams were constructed using the water titration method at room temperature, and the results were plotted using Software Origin® Pro 8.0. For each phase diagram, mixtures of surfactants and oil were prepared at weight ratios of 1 : 9, 2 : 8, 3 : 7, 4 : 6, 5 : 5, 6 : 4, 7 : 3, 8 : 2, and 9 : 1 (%w/w). These mixtures were titrated dropwise with water-propylene glycol (1 : 3) under mechanic stirring. After equilibration, the systems were visually characterized. Single-phase, transparent mixtures were designated as microemulsions [28].After data analyses of the pseudo-ternary phase diagrams, the microemulsion system was prepared by mixing babassu oil (12.2%) with the surfactants (48.8%) before adding the aqueous phase (39%) under magnetic stirring. After two days, the system was evaluated by complementary techniques, as described below.


### 2.10. Characterization of the Microemulsion

#### 2.10.1. Electrical Conductivity (EC)

EC was evaluated using a digital conductivity meter (mCA 150P, Tecnopon, São Paulo, Brazil) previously calibrated with a calibration solution (146.9μS/cm). The electrode was inserted directly into 10 mL of the formulation. The analysis was performed in triplicate at a temperature of 25±0.5°C.


#### 2.10.2. TEM

The sample was dropped onto a 300 mesh carbon-coated cooper grid and negatively stained with 2% phosphotungstic acid. The grid was analyzed in an FEI Tecnai Spirit Biotwin G2 microscope (Hillsboro, Oregon, USA) operated with 80 KV of accelerating voltage.


#### 2.10.3. SAXS

SAXS experiments were performed on the SAXS1 beamline of the Brazilian Synchrotron Light Laboratory (LNLS, São Paulo, Brazil), monitored with a photomultiplier, and detected on a Pilatus detector (300k Dectris) positioned at 836 mm that generated scattering wave vectors (q) from 0.13 to 2.5 nm−1. The wavelength of the incident X-ray beam (λ) was 0.155 nm−1. Background and parasitic scattering were determined by separate measurements on an empty holder and subtracted from the scattering data of the sample. The X-ray scattering was experimentally determined as a function of the scattering vector, q, whose modulus is given as follows:(2)q=4πsenθλ,where θ is half of the scattering angle (2θ).


#### 2.10.4. DSC

DSC analysis was performed using a Shimadzu DSC 50 (Kyoto, Japan) with 9-10 mg of sample under a nitrogen atmosphere with a flow rate of 50 mL/min. The melting temperature and enthalpy were calibrated with indium and zinc standards. The samples were analyzed from −50 to 60°C with a heating rate of 10°C/min.


#### 2.10.5. Rheological Analysis

The rheological behavior of the formulation was investigated on an Anton Paar Physica MCR502 oscillatory rheometer (Ashland, VA, USA) with cone and plate geometry (50 mm diameter). The gap between the cone and plate was set at 0.05 mm. The measurements were taken at 25°C over a shear rate (γ) range from 0.1 to 1000/s.


### 2.11. Statistical Analysis

Results were expressed as mean ± SEM and normal distributions were checked by Shapiro-Wilk test. Then, data were analyzed by one-way ANOVA followed by Tukey’s test using GraphPad Prism® 5.0 with significance set atp<0.05.


## 2.1. Chemicals

Phorbol 12-myristate 13-acetate (PMA), arachidonic acid (AA), ethyl phenylpropiolate (EPP), phenol, and capsaicin were purchased from Sigma Chemical Co. (St. Louis, MO, USA). The surfactants, Span 80 (sorbitan mono-oleate) and Tween® 80 (polyoxyethylene sorbitan monooleate), ethanol, 99.9% deuterated CDCl3, and Supelco® 37 Component FAME Mix were also obtained from Sigma Chemical Co. Kolliphor EL (polyoxyl castor oil) was from BASF SE (Ludwigshafen, Germany) and propylene glycol PA was purchased from Neon Comercial Ltda (São Paulo, Brazil).


## 2.2. Plant Material and Oil Extraction

Babassu fruits were collected from one palm tree in Araripe, located in the State of Ceará (Brazil), in July 2013. Botanical authentication was conducted by Olívia O. Cano, and a voucher specimen was deposited at the Herbarium of the Agronomic Institute of Pernambuco under number 90,472.The oil was extracted by a well-established method that is practiced by farmers in forested areas. After harvesting, the seeds were ground with a stone grinder, and the obtained paste was mixed well and allowed to stand overnight. On the next day, cold water was added to the paste, and the upper part was collected and placed on a fire to heat it until it started boiling. Subsequently, the liquid phase was separated from the oil meal using a tissue and heated until the water completely evaporated. Finally, the oil was filtered and used [18].


## 2.3. Physicochemical Characterization and Fatty Acid Profile

Traditionally extracted babassu oil was immediately analysed for some physicochemical properties as described by Adolfo Lutz Institute [19] as follows: relative density was determined in a 10 mL glass hydrometer (pycnometer) at 25°C; refractive index at 40°C was performed using a Abbé refractometer; peroxide values were calculated from the iodine release from potassium iodide and expressed as milliequivalents of active oxygen/kg of oil; rancidity (lipid oxidation) was determined by Kreis reaction using phloroglucinol in acid medium; acid values were expressed as milligrams of KOH required to neutralize the free acids of 1 g of oil, which was determined by titration in methanolic solution.The transesterification procedure of the oil was realized in accord with Metcalfe et al. [20]. The oil (150 mg) was mixed with 4.0 mL of NaOH in 0.50 M methanol and incubated at 100°C for 5 minutes. Next, 5.0 mL solution of BF3 (12%) in methanol was added in the mixture and was heated for 2 minutes. After cooling to room temperature, 5.0 mL of a saturated sodium chloride solution was added. The mixture was transferred to a separation funnel containing 20.0 mL of petroleum ether and vigorously stirred. After a period of rest, the aqueous phase was discarded and the ether phase was filtered. The solvent was evaporated in a rotary evaporator at 60°C and the residual solvent was removed with nitrogen flow. Methyl esters were solubilized in n-heptane before injection into the gas chromatographer [21].The samples were analyzed using an Agilent Technologies (Palo Alto, CA, USA) 5975C single quadrupole GC-MS equipped with a nonpolar HP-5MS (Agilent) fused silica capillary column (30 m × 0.25 mm i.d.; film thickness 0.25 mm). The oven was initially held at 150°C for 2 min, increased to 230°C by 5°C/min (held for 7 min), and finally increased to 260°C by 4°C/min. The final temperature was maintained for 7.5 min. The carrier gas was helium supplied at a constant flow of 1 mL/min and the split/splitless injector was maintained at 230°C. The applied ionization potential was 70 eV; the scan range was from 35 to 450m/z with scan rate of 0.5 scans/s. A standard fatty acid methyl ester mixture (Supelco 37 Component FAME Mix, USA) was used to identify the fatty acid methyl esters. Fatty acid data were expressed as percentage of total peak area.


## 2.4. NMR Analysis

The extracted oil (approximately 50 mg) was dissolved in 0.6 mL of CDCl3 and placed in a 5 mm NMR tube. The NMR analyses were performed on a Varian 400-VNMRS (Agilent Technologies, CA, USA) at 26°C, operating at the frequencies of 399.74 and 100.51 MHz for 1H and 13C, respectively. 1H NMR spectra were recorded with 2.5 s acquisition times, sweep widths of 6.4 kHz, 45° pulse angles, and 1 s delay times. C13{H1} NMR spectra were recorded with 3.04 s acquisition times, sweep widths of 21.5 kHz, 45° pulse angles, and 1 s delay times. For processing 13C spectra, zero filling and line broadening of 1 Hz were applied prior to Fourier transformation. The chemical shift scale was indirectly referred to tetramethylsilane (TMS) by the residual CHCl3 signal at 7.26 ppm for 1H spectra and the signal at 77.0 ppm for 13C spectra.


## 2.5. Topical Anti-Inflammatory Activity

### 2.5.1. Animals

Male Swiss and BALB/c mice (25–30 g,n=6) were provided by the Animal Facility of Federal University of Pernambuco. The animals were housed and kept in a room with controlled temperature (23±2°C) under a 12/12 h light/dark cycle with food and water ad libitum. Experiments were conducted according to the Guide for the Care and Use of Laboratory Animals from the US Department of Health and Human Services. The project had been previously approved by the Animal’s Ethics Committee of Federal University of Pernambuco (number 23076.039887/2014-51).


### 2.5.2. Ear Edema Measurement

For evaluation of ear weight, mice were euthanized and 6 mm diameter samples were taken from both ears using a biopsy punch (Richter®, Brazil). Each biopsy was weighed on a semi-micro analytical balance (AUW-D 220, Shimadzu, Japan). Ear edema (EE) was expressed as the increase in ear sample weight, using the following formula:(1)EEmg=wRE-wLE,where wRE is the weight obtained from the right ear sample (inflamed ear) and wLE is the weight obtained from the left ear sample (noninflamed ear).For all treatments, animals were anesthetized with 1% halothane. Right ears were then challenged with different phlogistic agents diluted in acetone (20μL). Babassu oil (1, 3, and 10 μL/ear) was applied topically in 20 μL acetone, while microemulsion was applied with no dilution. Dexamethasone or indomethacin (0.1 and 0.5 mg/ear, resp.) was used topically as a positive control. Ruthenium red (3 mg/kg, s.c.) was used as a positive control for capsaicin-induced ear edema.


### 2.5.3. PMA-Induced Ear Edema

Ear edema was induced by topical application of 20μL of PMA (2.5 μg/ear) in acetone on both sides of the right ear. Left ears received only acetone as a control. After 15 min, 10 μL of babassu oil (100 and 12.2%), babassu microemulsion, microemulsion vehicle (48.8% surfactants, 39% aqueous phase, and 12.2% water), 0.5% dexamethasone, or acetone were applied to both sides of the right and left ears. Six hours after PMA administration, mice were euthanized for ear edema measurement [22, 23]. To avoid misinterpretation of the results due to a possible barrier effect of babassu oil on ear surfaces, an oral treatment with babassu oil was also evaluated against PMA-induced ear edema. Shortly, mice were treated with babassu oil (100, 300, and 1000 mg/kg) or indomethacin (10 mg/kg, p.o.) 60 min before PMA administration [23].


### 2.5.4. Investigation of Babassu Oil Mode of Action on Ear Edema

For elucidation of the mechanisms underlying the topical anti-inflammatory activity of babassu oil on PMA-induced mice ear edema, different phlogistic agents were used to induce ear edema: arachidonic acid (2 mg/ear), ethyl phenylpropiolate 5% (20μL/ear), phenol 10% (20 μL/ear), and capsaicin (0.25 mg/ear). Immediately after acetone evaporation, babassu oil (10 μL/ear) or indomethacin (0.5 mg/ear)/dexamethasone (0.1 mg/ear) were topically applied on both sides of the right ear, while the left ear received 20 μL of the vehicle. Ruthenium red (3 mg/kg, s.c.) was used as positive control for capsaicin-induced ear edema and it was administered 30 min before capsaicin.After 1 h, mice were euthanized for ear edema measurement. Mice challenged with capsaicin were euthanized after 30 min of exposure. AA-induced ear edema was performed in BALB/c mice [23–25].


## 2.5.1. Animals

Male Swiss and BALB/c mice (25–30 g,n=6) were provided by the Animal Facility of Federal University of Pernambuco. The animals were housed and kept in a room with controlled temperature (23±2°C) under a 12/12 h light/dark cycle with food and water ad libitum. Experiments were conducted according to the Guide for the Care and Use of Laboratory Animals from the US Department of Health and Human Services. The project had been previously approved by the Animal’s Ethics Committee of Federal University of Pernambuco (number 23076.039887/2014-51).


## 2.5.2. Ear Edema Measurement

For evaluation of ear weight, mice were euthanized and 6 mm diameter samples were taken from both ears using a biopsy punch (Richter®, Brazil). Each biopsy was weighed on a semi-micro analytical balance (AUW-D 220, Shimadzu, Japan). Ear edema (EE) was expressed as the increase in ear sample weight, using the following formula:(1)EEmg=wRE-wLE,where wRE is the weight obtained from the right ear sample (inflamed ear) and wLE is the weight obtained from the left ear sample (noninflamed ear).For all treatments, animals were anesthetized with 1% halothane. Right ears were then challenged with different phlogistic agents diluted in acetone (20μL). Babassu oil (1, 3, and 10 μL/ear) was applied topically in 20 μL acetone, while microemulsion was applied with no dilution. Dexamethasone or indomethacin (0.1 and 0.5 mg/ear, resp.) was used topically as a positive control. Ruthenium red (3 mg/kg, s.c.) was used as a positive control for capsaicin-induced ear edema.


## 2.5.3. PMA-Induced Ear Edema

Ear edema was induced by topical application of 20μL of PMA (2.5 μg/ear) in acetone on both sides of the right ear. Left ears received only acetone as a control. After 15 min, 10 μL of babassu oil (100 and 12.2%), babassu microemulsion, microemulsion vehicle (48.8% surfactants, 39% aqueous phase, and 12.2% water), 0.5% dexamethasone, or acetone were applied to both sides of the right and left ears. Six hours after PMA administration, mice were euthanized for ear edema measurement [22, 23]. To avoid misinterpretation of the results due to a possible barrier effect of babassu oil on ear surfaces, an oral treatment with babassu oil was also evaluated against PMA-induced ear edema. Shortly, mice were treated with babassu oil (100, 300, and 1000 mg/kg) or indomethacin (10 mg/kg, p.o.) 60 min before PMA administration [23].


## 2.5.4. Investigation of Babassu Oil Mode of Action on Ear Edema

For elucidation of the mechanisms underlying the topical anti-inflammatory activity of babassu oil on PMA-induced mice ear edema, different phlogistic agents were used to induce ear edema: arachidonic acid (2 mg/ear), ethyl phenylpropiolate 5% (20μL/ear), phenol 10% (20 μL/ear), and capsaicin (0.25 mg/ear). Immediately after acetone evaporation, babassu oil (10 μL/ear) or indomethacin (0.5 mg/ear)/dexamethasone (0.1 mg/ear) were topically applied on both sides of the right ear, while the left ear received 20 μL of the vehicle. Ruthenium red (3 mg/kg, s.c.) was used as positive control for capsaicin-induced ear edema and it was administered 30 min before capsaicin.After 1 h, mice were euthanized for ear edema measurement. Mice challenged with capsaicin were euthanized after 30 min of exposure. AA-induced ear edema was performed in BALB/c mice [23–25].


## 2.6. Preparation of Emulsions

A series of oil-in-water emulsions with HLB values ranging from 4.5 to 15 was prepared with the surfactants Span 80 and Tween 80 at a 2% total blend concentration w/v, 93% water, and 5% babassu oil using the Griffin equation [26]. The aqueous phase (water plus Tween 80) and the oil phase (oil plus Span 80) were heated to 70±5°C separately. Both phases were mixed by the inversion method with mechanical stirring (9.000 rpm) for 5 min in ULTRA-TURRAX homogenizer equipment (Unique/DES500, São Paulo, Brazil). The samples were characterized 24 h after preparation.


## 2.7. Droplet Size Analysis by Dynamic Light Scattering (DLS)

The droplet size distribution of the dispersed phase of the emulsions was determined by DLS using the Nanotrac Wave equipment (Microtrac Inc., Montgomeryville, PA, USA) with measurement capability from 0.8 to 6500 nanometers. The data were calculated using the manufacturer’s software. Each emulsion was diluted with aqueous propylene glycol (1 : 100) and analyzed in triplicate.


## 2.8. Turbidimetric Method

The emulsions were diluted 1 : 25 with aqueous propylene glycol, and the percentage of transmission (%T) was measured at 600 nm using a spectrophotometer (UV-mini 1240, Shimadzu, Japan). With the blank control set at 100% transmission, the turbidity of the diluted emulsion was calculated as follows: turbidity = 100 − %T [27]. The reported results were the average of three determinations.


## 2.9. Construction of the Pseudo-Ternary Phase Diagram

Babassu oil, Span 80, and Kolliphor EL were selected as the oil phase and surfactants, respectively. Surfactant mixtures were tested at a ratio of 6 : 4 (w/w), as defined by the Griffin equation. The pseudo-ternary phase diagrams were constructed using the water titration method at room temperature, and the results were plotted using Software Origin® Pro 8.0. For each phase diagram, mixtures of surfactants and oil were prepared at weight ratios of 1 : 9, 2 : 8, 3 : 7, 4 : 6, 5 : 5, 6 : 4, 7 : 3, 8 : 2, and 9 : 1 (%w/w). These mixtures were titrated dropwise with water-propylene glycol (1 : 3) under mechanic stirring. After equilibration, the systems were visually characterized. Single-phase, transparent mixtures were designated as microemulsions [28].After data analyses of the pseudo-ternary phase diagrams, the microemulsion system was prepared by mixing babassu oil (12.2%) with the surfactants (48.8%) before adding the aqueous phase (39%) under magnetic stirring. After two days, the system was evaluated by complementary techniques, as described below.


## 2.10. Characterization of the Microemulsion

### 2.10.1. Electrical Conductivity (EC)

EC was evaluated using a digital conductivity meter (mCA 150P, Tecnopon, São Paulo, Brazil) previously calibrated with a calibration solution (146.9μS/cm). The electrode was inserted directly into 10 mL of the formulation. The analysis was performed in triplicate at a temperature of 25±0.5°C.


### 2.10.2. TEM

The sample was dropped onto a 300 mesh carbon-coated cooper grid and negatively stained with 2% phosphotungstic acid. The grid was analyzed in an FEI Tecnai Spirit Biotwin G2 microscope (Hillsboro, Oregon, USA) operated with 80 KV of accelerating voltage.


### 2.10.3. SAXS

SAXS experiments were performed on the SAXS1 beamline of the Brazilian Synchrotron Light Laboratory (LNLS, São Paulo, Brazil), monitored with a photomultiplier, and detected on a Pilatus detector (300k Dectris) positioned at 836 mm that generated scattering wave vectors (q) from 0.13 to 2.5 nm−1. The wavelength of the incident X-ray beam (λ) was 0.155 nm−1. Background and parasitic scattering were determined by separate measurements on an empty holder and subtracted from the scattering data of the sample. The X-ray scattering was experimentally determined as a function of the scattering vector, q, whose modulus is given as follows:(2)q=4πsenθλ,where θ is half of the scattering angle (2θ).


### 2.10.4. DSC

DSC analysis was performed using a Shimadzu DSC 50 (Kyoto, Japan) with 9-10 mg of sample under a nitrogen atmosphere with a flow rate of 50 mL/min. The melting temperature and enthalpy were calibrated with indium and zinc standards. The samples were analyzed from −50 to 60°C with a heating rate of 10°C/min.


### 2.10.5. Rheological Analysis

The rheological behavior of the formulation was investigated on an Anton Paar Physica MCR502 oscillatory rheometer (Ashland, VA, USA) with cone and plate geometry (50 mm diameter). The gap between the cone and plate was set at 0.05 mm. The measurements were taken at 25°C over a shear rate (γ) range from 0.1 to 1000/s.


## 2.10.1. Electrical Conductivity (EC)

EC was evaluated using a digital conductivity meter (mCA 150P, Tecnopon, São Paulo, Brazil) previously calibrated with a calibration solution (146.9μS/cm). The electrode was inserted directly into 10 mL of the formulation. The analysis was performed in triplicate at a temperature of 25±0.5°C.


## 2.10.2. TEM

The sample was dropped onto a 300 mesh carbon-coated cooper grid and negatively stained with 2% phosphotungstic acid. The grid was analyzed in an FEI Tecnai Spirit Biotwin G2 microscope (Hillsboro, Oregon, USA) operated with 80 KV of accelerating voltage.


## 2.10.3. SAXS

SAXS experiments were performed on the SAXS1 beamline of the Brazilian Synchrotron Light Laboratory (LNLS, São Paulo, Brazil), monitored with a photomultiplier, and detected on a Pilatus detector (300k Dectris) positioned at 836 mm that generated scattering wave vectors (q) from 0.13 to 2.5 nm−1. The wavelength of the incident X-ray beam (λ) was 0.155 nm−1. Background and parasitic scattering were determined by separate measurements on an empty holder and subtracted from the scattering data of the sample. The X-ray scattering was experimentally determined as a function of the scattering vector, q, whose modulus is given as follows:(2)q=4πsenθλ,where θ is half of the scattering angle (2θ).


## 2.10.4. DSC

DSC analysis was performed using a Shimadzu DSC 50 (Kyoto, Japan) with 9-10 mg of sample under a nitrogen atmosphere with a flow rate of 50 mL/min. The melting temperature and enthalpy were calibrated with indium and zinc standards. The samples were analyzed from −50 to 60°C with a heating rate of 10°C/min.


## 2.10.5. Rheological Analysis

The rheological behavior of the formulation was investigated on an Anton Paar Physica MCR502 oscillatory rheometer (Ashland, VA, USA) with cone and plate geometry (50 mm diameter). The gap between the cone and plate was set at 0.05 mm. The measurements were taken at 25°C over a shear rate (γ) range from 0.1 to 1000/s.


## 2.11. Statistical Analysis

Results were expressed as mean ± SEM and normal distributions were checked by Shapiro-Wilk test. Then, data were analyzed by one-way ANOVA followed by Tukey’s test using GraphPad Prism® 5.0 with significance set atp<0.05.


## 3. Results and Discussion

### 3.1. Physicochemical Characterization, GC-MS, and NMR Analyses of Babassu Oil

Physicochemical parameters of babassu oil and reference values are placed in Table1, while fatty acid composition obtained by GC-MS is showed in Table 2. The main fatty acids found were dodecanoic acid (lauric acid) (40.78%), 9-octadecenoic acid (oleic acid) (21.35%), tetradecanoic acid (myristic acid) (20.05%), and hexadecanoic acid (palmitic acid) (12.26%) (see SM Figure 2). These data are in accordance with previous published data and with reference standards of Brazilian regulatory agencies [29–31]. Differences in relative density and refractive index from reference values can be explained since reference values are derived from refined oils. Nevertheless, those parameters indicative of oil degradation, as acidity, rancidity, and peroxide value, are well below the established limits of quality.Table 1
Physicochemical parameters of babassu oil from Chapada do Araripe, Brazil.
Physicochemical parameters
Babassu oil (unrefined)
Reference value (refined oil)
Relative density (g/mL)
0.9210
0.9140–0.9170
Refractive index at 40°C
1.458
1.448–1.451
Acid value (mgKOH/g)
0.13
Max. 4
Peroxide value (meq/kg)
nd
Max. 15
Rancidity
Absent
Absent
nd: not detected.Table 2
Fatty acid composition of babassu oil from Chapada do Araripe, Brazil.
Skeleton
Compound
Area (%) ± St Dev
C12:0
Dodecanoic acid
40.78 ± 1.56
C13:0
Tridecanoic acid
0.03 ± 0.01
C14:0
Tetradecanoic acid
20.05 ± 0.27
C16:0
Hexadecanoic acid
12.26 ± 0.59
C18:2n6c
(Z,Z)-9,12-Octadecadienoic acid
2.39 ± 0.29
C18:1n9c
(Z)-9-Octadecenoic acid
21.35 ± 0.36
C18:0
Octadecanoic acid
2.64 ± 0.091H NMR and 13C NMR analyses were performed to characterize the components of the sample and determine the distribution of fatty acids in the glycerol backbone of babassu oil (Table 3) (see SM Figures 3 and 4). NMR signals were assigned according to the literature [31–35]. There are some advantages to using NMR spectroscopy to study fatty acid compositions: (a) the ability to directly operate on the oil sample without any chemical manipulation, and (b) NMR is the only direct instrumental method by which the positional distribution of fatty acids on glycerol can be specifically identified [36].Table 3
1H and 13C NMR chemical shift (δ) data of babassu oil in CDCl3.
Hydrogen
δ (ppm)
Carbon
δ (ppm)
CH=CH
5.33
C=O
173.2 and 172.8
CH-O
5.22
CH=CH
129.9 and 129.6
CH2-O
4.29
CH2-O
62.1
CH2-O
4.15
CH-O
68.8
C=C-CH2-C=C
2.74
C=C-CH2-C=C
27.2
CH2-C=O
2.30
CH2-C=O
34.1
CH2-C=C
2.01
CH2-C=C
31.8
CH2-CH2-C=O
1.61
CH2-CH2-C=O
24.8
C
H
_
2
n
1.28
CH2-CH3
22.6
CH3
0.87
C
_
H
2
n
29.7–28.8
CH3
14.0The signals at 5.33, 2.01, and 2.74 ppm were assigned to the olefinic hydrogens, the protons attached to the allylic carbons, and the protons attached to thebis-allylic carbons, respectively (Table 3). These signals revealed the presence of unsaturated fatty acids, such as oleic acid in the oil mixture. Moreover, the last signal indicated that a small amount of linoleic acid was present. Linolenic acid was not detected in this mixture because no signal was observed at 0.98 ppm, which corresponds to the terminal methyl group of the fatty acid [32]. These results agree with those obtained in the GC-MS spectra (Table 2). The full identification of every fatty acid component was not possible due to signal overlap in the spectra.13C NMR spectra provide information regarding the positional isomerism of fatty acids in the glycerol backbones of triacylglycerols (TAG), diacylglycerols (DAG), and monoacylglycerols (MAG) [35]. When analyzing the spectral region of the carbonyl group, only two signals at 173.2 and 172.8 ppm were observed (Table 3). This pattern indicated that the oil mixture includes only TAG, a fatty acid distributed at the sn-1,3 and sn-2 positions. Moreover, the spectral region of the acyl carbons between 60 and 73 ppm shows only two peaks (68.85 and 62.07 ppm) (Table 3), supporting that the sample only contains TAG isomers. The presence of unsaturated fatty acids that was observed in the 1H spectrum was confirmed in the 13C spectrum by the signals between 128.00 and 130.00 ppm. This region is characteristic of ethylenic carbons. The chemical shifts at 129.66 and 129.99 ppm indicated that the unsaturated fatty acids in the oil mixture were majority oleic acid, in agreement with the GC-MS results.


### 3.2. Topical Anti-Inflammatory Activity

PMA-induced ear edema is a useful model for screening topical anti-inflammatory compounds and/or plant extracts that act at a variety of levels. Skin inflammation induced by topical PMA administration is mediated trough protein kinase C (PKC) activation of NF-κB, with production of tumor necrosis factor-α (TNF-α), cyclooxygenase-2 (COX-2), and prostaglandin E2 (PGE2) [37–39]. PMA given to mouse ears induces mast cell infiltration with release of mediators that increase vascular permeability and promote neutrophil influx [40, 41]. Anti-inflammatory efficacy of evaluated compounds implies mainly interference of arachidonic acid metabolism, since phospholipase A2 (PLA2), COX, and lipoxygenase (LOX) inhibitors have been shown to inhibit PMA-induced inflammation [23, 42].Babassu oil (3 and 10μL/ear) was able to inhibit PMA-induced ear edema in 19.1 (p<0.05) and 54.1% (p<0.001), respectively. Lauric acid (4 mg/ear), the major fatty acid of babassu oil, showed 90.3% of inhibition (p<0.001), while dexamethasone inhibited the ear edema in 79.7% (p<0.001) (Figure 1(a)). To avoid misinterpretation of the results due to a possible barrier effect of babassu oil on ear surfaces, an oral treatment with babassu oil was also evaluated against PMA-induced ear edema. When given orally, babassu oil (100, 300, and 1000 mg/kg) showed 23.5 (p<0.05), 39.7, and 51.9% of edema inhibition (p<0.001), while indomethacin (10 mg/kg) inhibited the edema in 62.0% (p<0.001) (Figure 1(b)).Figure 1
(a) Topical and (b) systemic anti-inflammatory activity of babassu oil in PMA-induced ear edema. Results are expressed as the mean ± SEM and analyzed by ANOVA followed by Tukey’s test withp set at 0.05. Different letters show statistical differences between groups (p<0.05). (−) Negative control (acetone); babassu oil (μL/ear in (a)) and (mg/kg, p.o. in (b)); (LA) lauric acid (mg/ear); (Dexa) dexamethasone (mg/ear), and (Indo) indomethacin (mg/kg, p.o.).
(a)
(b)It is worth to comment that the high content of lauric acid in babassu oil defines the importance of this study, since its antibacterial and anti-inflammatory activities are described in the literature [43–45], through inhibition of MAPK pathway and subsequent NF-κB activation, which are prominent contributors to the production of proinflammatory cytokines and chronic inflammatory responses [46], including in PMA-induced ear edema model. After topical administration, lauric acid penetrates the skin and accumulates in the dermis [47].To further elucidate the topical anti-inflammatory activity of babassu oil, different phlogistic agents-induced ear edema was performed. Arachidonic acid-induced ear edema is a useful tool to identify compounds that interfere in eicosanoid pathway, such as COX and LOX inhibitors. However, this model is not sensitive to PLA2 inhibitors such as glucocorticoids [23, 48–50]. Here, both babassu oil (10 μL/ear) and lauric acid (4 mg/ear) inhibited AA-induced ear edema by 78.5 and 61.6%, respectively (p<0.001). Indomethacin (0.5 mg/ear), a nonselective inhibitor of COX-1/2, decreased the ear edema by 65.4% (p<0.001) (Figure 2(a)). Short-chain fatty acids (C10–C12), which include lauric acid, are reported as mid-range inhibitors of COX-1 (about 50–60% of inhibition) and COX-2 (about 25–30% inhibition) in vitro [51].Figure 2
Topical anti-inflammatory activity of babassu oil and lauric acid in (a) arachidonic acid; (b) ethyl phenylpropiolate; and (c) phenol-induced ear edema. Results are expressed as the mean ± SEM and analyzed by ANOVA followed by Tukey’s test withp set at 0.05. Different letters show statistical differences between groups (p<0.05). (−) Negative control (acetone); (BA) babassu oil (μL/ear); (LA) lauric acid (mg/ear); (Indo) indomethacin (mg/ear); and (Dexa) dexamethasone (mg/ear).
(a)
(b)
(c)Barbosa et al. [52] demonstrated that babassu oil decreased ischemia-induced macromolecular leakage in postcapillary venules and inhibited histamine-induced microvascular permeability increase in hamster cheek pouch. To verify if the same occurs in skin, instead of mucosa, ethyl phenylpropiolate-induced ear edema was performed. EPP administration immediately increases vascular permeability, with blood flow increasing somewhat slowly. Histamine, serotonin (5-HT), kinins, and prostaglandins combined actions on vascular permeability seemed to be involved in early EPP response [53]. Babassu oil (10 μL/ear) and lauric acid (4 mg/ear) inhibited EPP-induced ear edema by 82.0 and 71.8% (p<0.001), respectively, in accordance with the results of Barbosa et al. [52]. Dexamethasone (0.1 mg/ear), used as positive control, inhibited edema formation by 65.6% (p<0.001) (Figure 2(b)).Phenol-induced skin inflammation is very like human contact dermatitis processes [54]. Phenol disrupts the plasma membranes of keratinocytes in the skin, resulting in release of preformed cytokines (IL-1α, IL-8, and TNF-α). These cytokines stimulate the production of reactive oxygen species (ROS) and AA metabolites amplifying the inflammatory process [54–56]. Herein, babassu oil (10 μL/ear) and lauric acid (4 mg/ear) inhibited phenol-induced ear edema by 89.8 and 90.0% (p<0.001), respectively. Dexamethasone (0.1 mg/ear), used as positive control due to its membrane stabilizing effect, inhibited edema formation by 91.0% (p<0.001) (Figure 2(c)).Topical administration of capsaicin releases proinflammatory mediators such as substance P and histamine, due to TRPV-1 activation, which result in an immediate vasodilation and erythema followed by edema. Maximum edema is achieved within 30 minutes after capsaicin administration [57, 58]. Neither babassu oil (10 μL/ear) nor lauric acid (4 mg/ear) was able to inhibit capsaicin-induced ear edema. Ruthenium red (3 mg/kg, s.c.), given 30 min before capsaicin administration, reduced the ear edema by 75.3% (p<0.01) (data not shown).


### 3.3. HLB Value and Microemulsion Formulation

The HLB number is a semiempirical scale for selecting surfactants [26]. The corrected HLB of the selected surfactant or blend of surfactants that match the HLB of the selected oil provides the lowest interface tension between the oil and water phases and gives the system stability [28]. In this context, the most stable emulsion is obtained in system with the smallest droplet size. Analysis by DLS showed the smallest mean droplet diameters in babassu oil emulsions at an HLB of 8.0 (2.3882 μm).The maximum turbidity values are the same HLB value at which the mean droplet diameter is minimal [27]. Here, we found that the highest mean turbidity values for babassu oil emulsions were obtained at HLB values from 8.0 to 10.0 (0.8–1.06). The correlation coefficient (Pearson r) between the turbidity values and the mean droplet size for the emulsion with an HLB value of 8.0 was 0.275 (r2=0.075624), showing a positive correlation (p<0.05, ANOVA followed by Tukey’s test).Using the HLB value required for the babassu oil (HLB 8.0), Span 80 and Kolliphor EL were selected as the surfactants at a ratio of 6 : 4, respectively, because this blend had an HLB value equal to that determined for the oil. The aqueous phase was composed of water and propylene glycol (1 : 3) and the oil phase was babassu oil. In most cases, single-chain surfactants alone are unable to reduce the interfacial tension sufficiently to form a microemulsion. Propylene glycol was added as a cosurfactant to decrease the interfacial tension and increase the fluidity of the interface. In this case, part of the propylene glycol content was incorporated into the surfactant layer, and the other part decreased the polarity of the water by dissolving in the water. However, a higher amount of propylene glycol molecules favors formation of a bicontinuous microemulsion and avoids rigid structures, such as gels and liquid-crystals [59].The transparent liquid systems formed by the pseudo-ternary phase diagram can be used to obtain concentration ranges of babassu oil, emulsifiers, and the aqueous phase for microemulsion formulations. A system composed of 39% aqueous phase, 12.2% oil phase, and 48.8% surfactants (Figure3, white dot) was selected for physical and morphological characterization. The selected system exhibited the typical characteristics of a microemulsion system, such as slight viscosity, transparency, and stability. In the diagram, this formulation is found near the borderline of o/w and bicontinuous microemulsions, which may indicate a transitional phase structure. In addition, the ratio of system components favors the formation of oil droplets surrounded by water, suitable for biological applications.Figure 3
Pseudo-ternary phase diagram (8 : 2) of the babassu oil microemulsion. The black region includes the microemulsion systems (ME) and the selected formulation (white dot). OLE, opaque liquid emulsions; MLE, milk liquid emulsions.


### 3.4. Electrical Conductivity

Electrical conductivity is commonly used to characterize the microstructure transitions that occur in microemulsions, that is, transformation from water-entrapped systems to intermediate structures and then to water continuous microstructures. Conductivity is low for reverse structures in nonconducting oil media that have little interactions with each other. When more water is added to the system, the conductive droplets begin to contact one another and form other structures, resulting in increased EC [60]. This phenomenon may have occurred in the babassu microemulsion because its conductivity value was 20.96 μS/cm.


### 3.5. Morphological Analysis

The ultrastructure of the babassu microemulsion was investigated using transmission electron microscopy (Figure4). We observed clusters of nanodroplets filled with oil that were surrounded by an electron dense material composed of the surfactant interface bonded to the aqueous phase. Most of the oil droplets were connected side by side, but some of them were merging, indicating a transition to an o/w microemulsion. In addition, thick peripheral layers surrounding the nanodroplet network were sometimes observed, which could be related to displacement of the water and emulsifiers from the inner phase during system transformation.Figure 4
The ultrastructure of the babassu microemulsion that shows clusters of nanodroplets filled with oil, some of which have merged with one another and are surrounded by the surfactant interface and aqueous phase.The microemulsion composition and individual characteristics of components define the phase morphology of the system. As the concentration of water increases, the droplets increase in size and eventually form a cluster that is considered infinite. At this stage, the microemulsion possesses a bicontinuous structure. Further addition of the water phase transforms the bicontinuous system into an o/w microemulsion, where the droplets of the organic phase are surrounded by the water bath, and the interface is composed of surfactant species. During each of these transition steps, morphological phases in intermediate regions may be formed, and the stability of the system depends on thermodynamic conditions [60–62].


### 3.6. SAXS Measurements

SAXS is a well-established technique used to investigate the morphology, shape, and size of a multiphase sample, namely, aggregates dispersed in liquids, to obtain structural information regarding inhomogeneities based on the difference of electron density in the samples. This technique provides them with a characteristic length on the order of tens to hundreds of Angstroms (Å) [63, 64].Figure5(a) shows the experimental data simulated according to (3) (see SM). The proposed model fits the experimental data, which present droplets sizes from 5 to 15 nm with the majority size of approximately 8.7 nm.Figure 5
(a) Scattering intensityI of the SAXS measurements as a function of the scattering vector q for the babassu oil microemulsion. The solid line is the fit according to supplementary file procedure (Equation (S1)). The spherical shell had an average outer radius of R=8.7 nm and inner radius of vR=3.48±0.02 nm. The normalized size distribution P(r) as a function of the overall radius (R) is also shown. (b) Structure factor S(q) for a hard sphere interaction potential of the babassu microemulsion with an average correlation distance (RHS) of 12±0.01 nm and a volume fraction of 0.14±0.001.
(a)
(b)The scattering length density difference between the shell and matrix (Δη) for the microemulsion was 8.5±0.03 nm, and the ν value was 0.4±0.01. The SAXS results showed that the structure had a thin transition layer between the oil and aqueous phases. In this case, the surfactant hydrophobic chains interacted with babassu oil, which was observed as a small transition region that surrounded the oil droplets in the TEM micrograph (see Figure 4). S(q) is the Fourier transform of the pair correlation function, which in turn depends on the pair potential between micelles. However, the Percus-Yevick approximation provides an analytical expression for S(q) for monodisperse particles acting as hard spheres [65]. The structure factor was estimated using the monodisperse approach. This method only multiplies the size averaged form factor with the structure factor. The interaction potentials between the particles are assumed to be spherical, symmetric, and independent of the particle size [66, 67]. Figure 5(b) shows the average structure factor for the microemulsion sample. The peak at approximately q~0.25 nm−1 in the S(q) curve indicates the correlation distance between the oil droplets. Besides, the other peaks at around q~0.50 and 0.78 nm−1 show that the average interaction potential distance between the oil droplets was approximately 12±0.01 nm.


### 3.7. DSC Analysis

The thermal behaviors of water in the microemulsion system were investigated by DSC and compared with that of pure water (Figure6). Initially, the samples were cooled to −50°C and then heated to 60°C at a rate of 10°C/min. The thermal transition for the propylene glycol sample was not observed in the temperature range. Pure water showed an endothermic transition near 0°C and enthalpy of fusion of approximately 330 J/g [68]. Pure babassu oil showed two endothermic peaks at 7 and 25°C that were related to the melting temperatures of fatty acid components [69]. The multiple peaks that were observed for the oil sample are due to the polymorphism of different fatty acid. Most vegetable oils can be found in at least three crystalline forms, designated as α, β′, and β [70, 71]. The enthalpy of fusion of pure oil is approximately 100±0.02 J/g, whereas the microemulsion sample showed a broad endothermic melt (fusion) transition in two temperature regions at 11 and 18°C (Figure 6, inset). It is essential to note that the presence of a nearby surface alters the thermodynamic properties, such as freezing point, melting point, and enthalpy [72, 73]. The endothermic transition was not observed at the same position as pure water. This effect is related to the partial miscibility of the surfactants and propylene glycol with water [74]. The literature has shown that the melting temperature deviation in relation to pure water depends on the nature of the interaction between the surfactant and water [72, 73, 75]. When the surfactant concentration is low, the transition of pure water is observed at approximately 0°C. Therefore, when we are dealing with systems in which the interactions depend on the concentrations, the surfactant properties can strongly contribute to the melting temperature and fusion enthalpy. For this reason, the microemulsion enthalpy decreased by 9.5±0.2 J/g when compared with water and pure oil.Figure 6
DSC thermographs for the babassu microemulsion, showing a broad endothermic melt transition in two temperature regions (inset).The presence of two melting peaks is a characteristic behavior of a bicontinuous microemulsion in situations where the oil phase is composed of a single fatty acid [60, 76–78]. However, when the oil phase consists of a triglyceride molecule, which is composed of various fatty acids, this behavior depends on the interactions of each fatty acid with the surfactants species. The first peak in the DSC thermogram of the microemulsion (~11°C) is related to the amount of free water. The second peak (~18°C) refers to the fusion of the oil phase in microemulsion. This thermal transition is shifted to lower temperatures because the fatty acids interact with the surfactant through the nonpolar head.


### 3.8. Rheological Behavior of the System

The rheological behavior of the babassu microemulsion is demonstrated in SM (FigureS5). The shear stress is proportional to increased shear rate, which is characteristic of Newtonian behavior. The profile of shear stress versus shear rate for Newtonian liquids yields a straight line, which is the expected behavior of microemulsions [79–81]. The average viscosity value for the babassu microemulsion was approximately 0.29±0.1 Pas.


### 3.9. Topical Anti-Inflammatory Activity of Babassu Microemulsion

The topical anti-inflammatory activity of the babassu microemulsion compared with pure babassu oil is shown in Figure7. Pure babassu oil and 12.2% babassu oil diluted in acetone inhibited the PMA-induced ear edema by 58.0% (p<0.001) and 17.5% (not significant), respectively. The babassu microemulsion inhibited ear edema by 66.2% (p<0.001), showing an enhanced activity promoted by the microemulsification of babassu oil, since babassu oil at 12.2% (final concentration in the microemulsion) did not have a significant effect on this experimental model. Dexamethasone (0.1 mg/ear) inhibited PMA-induced ear edema by 78.4% (p<0.001).Figure 7
Topical anti-inflammatory activity of babassu oil and microemulsion in PMA-induced ear edema. Results are expressed as the mean ± SEM and analyzed by ANOVA followed by Tukey’s test withp set at 0.05. Different letters show statistical differences between groups (p<0.05). (−) Negative control (acetone); (V) microemulsion vehicle (48.8% surfactants, 39% aqueous phase, and 12.2% water); babassu oil (μL/ear); (ME) babassu microemulsion (μL/ear); and (Dexa) dexamethasone (mg/ear).Skin permeation enhancement by microemulsions has been widely studied for several anti-inflammatory drugs, for example, indomethacin, aspirin, and rofecoxib [82–84]. Microemulsification of babassu oil seems to enhance skin permeation of anti-inflammatory active compounds found in the oil, reaching the same percentages of edema inhibition as pure babassu oil with a much lower oil concentration.


## 3.1. Physicochemical Characterization, GC-MS, and NMR Analyses of Babassu Oil

Physicochemical parameters of babassu oil and reference values are placed in Table1, while fatty acid composition obtained by GC-MS is showed in Table 2. The main fatty acids found were dodecanoic acid (lauric acid) (40.78%), 9-octadecenoic acid (oleic acid) (21.35%), tetradecanoic acid (myristic acid) (20.05%), and hexadecanoic acid (palmitic acid) (12.26%) (see SM Figure 2). These data are in accordance with previous published data and with reference standards of Brazilian regulatory agencies [29–31]. Differences in relative density and refractive index from reference values can be explained since reference values are derived from refined oils. Nevertheless, those parameters indicative of oil degradation, as acidity, rancidity, and peroxide value, are well below the established limits of quality.Table 1
Physicochemical parameters of babassu oil from Chapada do Araripe, Brazil.
Physicochemical parameters
Babassu oil (unrefined)
Reference value (refined oil)
Relative density (g/mL)
0.9210
0.9140–0.9170
Refractive index at 40°C
1.458
1.448–1.451
Acid value (mgKOH/g)
0.13
Max. 4
Peroxide value (meq/kg)
nd
Max. 15
Rancidity
Absent
Absent
nd: not detected.Table 2
Fatty acid composition of babassu oil from Chapada do Araripe, Brazil.
Skeleton
Compound
Area (%) ± St Dev
C12:0
Dodecanoic acid
40.78 ± 1.56
C13:0
Tridecanoic acid
0.03 ± 0.01
C14:0
Tetradecanoic acid
20.05 ± 0.27
C16:0
Hexadecanoic acid
12.26 ± 0.59
C18:2n6c
(Z,Z)-9,12-Octadecadienoic acid
2.39 ± 0.29
C18:1n9c
(Z)-9-Octadecenoic acid
21.35 ± 0.36
C18:0
Octadecanoic acid
2.64 ± 0.091H NMR and 13C NMR analyses were performed to characterize the components of the sample and determine the distribution of fatty acids in the glycerol backbone of babassu oil (Table 3) (see SM Figures 3 and 4). NMR signals were assigned according to the literature [31–35]. There are some advantages to using NMR spectroscopy to study fatty acid compositions: (a) the ability to directly operate on the oil sample without any chemical manipulation, and (b) NMR is the only direct instrumental method by which the positional distribution of fatty acids on glycerol can be specifically identified [36].Table 3
1H and 13C NMR chemical shift (δ) data of babassu oil in CDCl3.
Hydrogen
δ (ppm)
Carbon
δ (ppm)
CH=CH
5.33
C=O
173.2 and 172.8
CH-O
5.22
CH=CH
129.9 and 129.6
CH2-O
4.29
CH2-O
62.1
CH2-O
4.15
CH-O
68.8
C=C-CH2-C=C
2.74
C=C-CH2-C=C
27.2
CH2-C=O
2.30
CH2-C=O
34.1
CH2-C=C
2.01
CH2-C=C
31.8
CH2-CH2-C=O
1.61
CH2-CH2-C=O
24.8
C
H
_
2
n
1.28
CH2-CH3
22.6
CH3
0.87
C
_
H
2
n
29.7–28.8
CH3
14.0The signals at 5.33, 2.01, and 2.74 ppm were assigned to the olefinic hydrogens, the protons attached to the allylic carbons, and the protons attached to thebis-allylic carbons, respectively (Table 3). These signals revealed the presence of unsaturated fatty acids, such as oleic acid in the oil mixture. Moreover, the last signal indicated that a small amount of linoleic acid was present. Linolenic acid was not detected in this mixture because no signal was observed at 0.98 ppm, which corresponds to the terminal methyl group of the fatty acid [32]. These results agree with those obtained in the GC-MS spectra (Table 2). The full identification of every fatty acid component was not possible due to signal overlap in the spectra.13C NMR spectra provide information regarding the positional isomerism of fatty acids in the glycerol backbones of triacylglycerols (TAG), diacylglycerols (DAG), and monoacylglycerols (MAG) [35]. When analyzing the spectral region of the carbonyl group, only two signals at 173.2 and 172.8 ppm were observed (Table 3). This pattern indicated that the oil mixture includes only TAG, a fatty acid distributed at the sn-1,3 and sn-2 positions. Moreover, the spectral region of the acyl carbons between 60 and 73 ppm shows only two peaks (68.85 and 62.07 ppm) (Table 3), supporting that the sample only contains TAG isomers. The presence of unsaturated fatty acids that was observed in the 1H spectrum was confirmed in the 13C spectrum by the signals between 128.00 and 130.00 ppm. This region is characteristic of ethylenic carbons. The chemical shifts at 129.66 and 129.99 ppm indicated that the unsaturated fatty acids in the oil mixture were majority oleic acid, in agreement with the GC-MS results.


## 3.2. Topical Anti-Inflammatory Activity

PMA-induced ear edema is a useful model for screening topical anti-inflammatory compounds and/or plant extracts that act at a variety of levels. Skin inflammation induced by topical PMA administration is mediated trough protein kinase C (PKC) activation of NF-κB, with production of tumor necrosis factor-α (TNF-α), cyclooxygenase-2 (COX-2), and prostaglandin E2 (PGE2) [37–39]. PMA given to mouse ears induces mast cell infiltration with release of mediators that increase vascular permeability and promote neutrophil influx [40, 41]. Anti-inflammatory efficacy of evaluated compounds implies mainly interference of arachidonic acid metabolism, since phospholipase A2 (PLA2), COX, and lipoxygenase (LOX) inhibitors have been shown to inhibit PMA-induced inflammation [23, 42].Babassu oil (3 and 10μL/ear) was able to inhibit PMA-induced ear edema in 19.1 (p<0.05) and 54.1% (p<0.001), respectively. Lauric acid (4 mg/ear), the major fatty acid of babassu oil, showed 90.3% of inhibition (p<0.001), while dexamethasone inhibited the ear edema in 79.7% (p<0.001) (Figure 1(a)). To avoid misinterpretation of the results due to a possible barrier effect of babassu oil on ear surfaces, an oral treatment with babassu oil was also evaluated against PMA-induced ear edema. When given orally, babassu oil (100, 300, and 1000 mg/kg) showed 23.5 (p<0.05), 39.7, and 51.9% of edema inhibition (p<0.001), while indomethacin (10 mg/kg) inhibited the edema in 62.0% (p<0.001) (Figure 1(b)).Figure 1
(a) Topical and (b) systemic anti-inflammatory activity of babassu oil in PMA-induced ear edema. Results are expressed as the mean ± SEM and analyzed by ANOVA followed by Tukey’s test withp set at 0.05. Different letters show statistical differences between groups (p<0.05). (−) Negative control (acetone); babassu oil (μL/ear in (a)) and (mg/kg, p.o. in (b)); (LA) lauric acid (mg/ear); (Dexa) dexamethasone (mg/ear), and (Indo) indomethacin (mg/kg, p.o.).
(a)
(b)It is worth to comment that the high content of lauric acid in babassu oil defines the importance of this study, since its antibacterial and anti-inflammatory activities are described in the literature [43–45], through inhibition of MAPK pathway and subsequent NF-κB activation, which are prominent contributors to the production of proinflammatory cytokines and chronic inflammatory responses [46], including in PMA-induced ear edema model. After topical administration, lauric acid penetrates the skin and accumulates in the dermis [47].To further elucidate the topical anti-inflammatory activity of babassu oil, different phlogistic agents-induced ear edema was performed. Arachidonic acid-induced ear edema is a useful tool to identify compounds that interfere in eicosanoid pathway, such as COX and LOX inhibitors. However, this model is not sensitive to PLA2 inhibitors such as glucocorticoids [23, 48–50]. Here, both babassu oil (10 μL/ear) and lauric acid (4 mg/ear) inhibited AA-induced ear edema by 78.5 and 61.6%, respectively (p<0.001). Indomethacin (0.5 mg/ear), a nonselective inhibitor of COX-1/2, decreased the ear edema by 65.4% (p<0.001) (Figure 2(a)). Short-chain fatty acids (C10–C12), which include lauric acid, are reported as mid-range inhibitors of COX-1 (about 50–60% of inhibition) and COX-2 (about 25–30% inhibition) in vitro [51].Figure 2
Topical anti-inflammatory activity of babassu oil and lauric acid in (a) arachidonic acid; (b) ethyl phenylpropiolate; and (c) phenol-induced ear edema. Results are expressed as the mean ± SEM and analyzed by ANOVA followed by Tukey’s test withp set at 0.05. Different letters show statistical differences between groups (p<0.05). (−) Negative control (acetone); (BA) babassu oil (μL/ear); (LA) lauric acid (mg/ear); (Indo) indomethacin (mg/ear); and (Dexa) dexamethasone (mg/ear).
(a)
(b)
(c)Barbosa et al. [52] demonstrated that babassu oil decreased ischemia-induced macromolecular leakage in postcapillary venules and inhibited histamine-induced microvascular permeability increase in hamster cheek pouch. To verify if the same occurs in skin, instead of mucosa, ethyl phenylpropiolate-induced ear edema was performed. EPP administration immediately increases vascular permeability, with blood flow increasing somewhat slowly. Histamine, serotonin (5-HT), kinins, and prostaglandins combined actions on vascular permeability seemed to be involved in early EPP response [53]. Babassu oil (10 μL/ear) and lauric acid (4 mg/ear) inhibited EPP-induced ear edema by 82.0 and 71.8% (p<0.001), respectively, in accordance with the results of Barbosa et al. [52]. Dexamethasone (0.1 mg/ear), used as positive control, inhibited edema formation by 65.6% (p<0.001) (Figure 2(b)).Phenol-induced skin inflammation is very like human contact dermatitis processes [54]. Phenol disrupts the plasma membranes of keratinocytes in the skin, resulting in release of preformed cytokines (IL-1α, IL-8, and TNF-α). These cytokines stimulate the production of reactive oxygen species (ROS) and AA metabolites amplifying the inflammatory process [54–56]. Herein, babassu oil (10 μL/ear) and lauric acid (4 mg/ear) inhibited phenol-induced ear edema by 89.8 and 90.0% (p<0.001), respectively. Dexamethasone (0.1 mg/ear), used as positive control due to its membrane stabilizing effect, inhibited edema formation by 91.0% (p<0.001) (Figure 2(c)).Topical administration of capsaicin releases proinflammatory mediators such as substance P and histamine, due to TRPV-1 activation, which result in an immediate vasodilation and erythema followed by edema. Maximum edema is achieved within 30 minutes after capsaicin administration [57, 58]. Neither babassu oil (10 μL/ear) nor lauric acid (4 mg/ear) was able to inhibit capsaicin-induced ear edema. Ruthenium red (3 mg/kg, s.c.), given 30 min before capsaicin administration, reduced the ear edema by 75.3% (p<0.01) (data not shown).


## 3.3. HLB Value and Microemulsion Formulation

The HLB number is a semiempirical scale for selecting surfactants [26]. The corrected HLB of the selected surfactant or blend of surfactants that match the HLB of the selected oil provides the lowest interface tension between the oil and water phases and gives the system stability [28]. In this context, the most stable emulsion is obtained in system with the smallest droplet size. Analysis by DLS showed the smallest mean droplet diameters in babassu oil emulsions at an HLB of 8.0 (2.3882 μm).The maximum turbidity values are the same HLB value at which the mean droplet diameter is minimal [27]. Here, we found that the highest mean turbidity values for babassu oil emulsions were obtained at HLB values from 8.0 to 10.0 (0.8–1.06). The correlation coefficient (Pearson r) between the turbidity values and the mean droplet size for the emulsion with an HLB value of 8.0 was 0.275 (r2=0.075624), showing a positive correlation (p<0.05, ANOVA followed by Tukey’s test).Using the HLB value required for the babassu oil (HLB 8.0), Span 80 and Kolliphor EL were selected as the surfactants at a ratio of 6 : 4, respectively, because this blend had an HLB value equal to that determined for the oil. The aqueous phase was composed of water and propylene glycol (1 : 3) and the oil phase was babassu oil. In most cases, single-chain surfactants alone are unable to reduce the interfacial tension sufficiently to form a microemulsion. Propylene glycol was added as a cosurfactant to decrease the interfacial tension and increase the fluidity of the interface. In this case, part of the propylene glycol content was incorporated into the surfactant layer, and the other part decreased the polarity of the water by dissolving in the water. However, a higher amount of propylene glycol molecules favors formation of a bicontinuous microemulsion and avoids rigid structures, such as gels and liquid-crystals [59].The transparent liquid systems formed by the pseudo-ternary phase diagram can be used to obtain concentration ranges of babassu oil, emulsifiers, and the aqueous phase for microemulsion formulations. A system composed of 39% aqueous phase, 12.2% oil phase, and 48.8% surfactants (Figure3, white dot) was selected for physical and morphological characterization. The selected system exhibited the typical characteristics of a microemulsion system, such as slight viscosity, transparency, and stability. In the diagram, this formulation is found near the borderline of o/w and bicontinuous microemulsions, which may indicate a transitional phase structure. In addition, the ratio of system components favors the formation of oil droplets surrounded by water, suitable for biological applications.Figure 3
Pseudo-ternary phase diagram (8 : 2) of the babassu oil microemulsion. The black region includes the microemulsion systems (ME) and the selected formulation (white dot). OLE, opaque liquid emulsions; MLE, milk liquid emulsions.


## 3.4. Electrical Conductivity

Electrical conductivity is commonly used to characterize the microstructure transitions that occur in microemulsions, that is, transformation from water-entrapped systems to intermediate structures and then to water continuous microstructures. Conductivity is low for reverse structures in nonconducting oil media that have little interactions with each other. When more water is added to the system, the conductive droplets begin to contact one another and form other structures, resulting in increased EC [60]. This phenomenon may have occurred in the babassu microemulsion because its conductivity value was 20.96 μS/cm.


## 3.5. Morphological Analysis

The ultrastructure of the babassu microemulsion was investigated using transmission electron microscopy (Figure4). We observed clusters of nanodroplets filled with oil that were surrounded by an electron dense material composed of the surfactant interface bonded to the aqueous phase. Most of the oil droplets were connected side by side, but some of them were merging, indicating a transition to an o/w microemulsion. In addition, thick peripheral layers surrounding the nanodroplet network were sometimes observed, which could be related to displacement of the water and emulsifiers from the inner phase during system transformation.Figure 4
The ultrastructure of the babassu microemulsion that shows clusters of nanodroplets filled with oil, some of which have merged with one another and are surrounded by the surfactant interface and aqueous phase.The microemulsion composition and individual characteristics of components define the phase morphology of the system. As the concentration of water increases, the droplets increase in size and eventually form a cluster that is considered infinite. At this stage, the microemulsion possesses a bicontinuous structure. Further addition of the water phase transforms the bicontinuous system into an o/w microemulsion, where the droplets of the organic phase are surrounded by the water bath, and the interface is composed of surfactant species. During each of these transition steps, morphological phases in intermediate regions may be formed, and the stability of the system depends on thermodynamic conditions [60–62].


## 3.6. SAXS Measurements

SAXS is a well-established technique used to investigate the morphology, shape, and size of a multiphase sample, namely, aggregates dispersed in liquids, to obtain structural information regarding inhomogeneities based on the difference of electron density in the samples. This technique provides them with a characteristic length on the order of tens to hundreds of Angstroms (Å) [63, 64].Figure5(a) shows the experimental data simulated according to (3) (see SM). The proposed model fits the experimental data, which present droplets sizes from 5 to 15 nm with the majority size of approximately 8.7 nm.Figure 5
(a) Scattering intensityI of the SAXS measurements as a function of the scattering vector q for the babassu oil microemulsion. The solid line is the fit according to supplementary file procedure (Equation (S1)). The spherical shell had an average outer radius of R=8.7 nm and inner radius of vR=3.48±0.02 nm. The normalized size distribution P(r) as a function of the overall radius (R) is also shown. (b) Structure factor S(q) for a hard sphere interaction potential of the babassu microemulsion with an average correlation distance (RHS) of 12±0.01 nm and a volume fraction of 0.14±0.001.
(a)
(b)The scattering length density difference between the shell and matrix (Δη) for the microemulsion was 8.5±0.03 nm, and the ν value was 0.4±0.01. The SAXS results showed that the structure had a thin transition layer between the oil and aqueous phases. In this case, the surfactant hydrophobic chains interacted with babassu oil, which was observed as a small transition region that surrounded the oil droplets in the TEM micrograph (see Figure 4). S(q) is the Fourier transform of the pair correlation function, which in turn depends on the pair potential between micelles. However, the Percus-Yevick approximation provides an analytical expression for S(q) for monodisperse particles acting as hard spheres [65]. The structure factor was estimated using the monodisperse approach. This method only multiplies the size averaged form factor with the structure factor. The interaction potentials between the particles are assumed to be spherical, symmetric, and independent of the particle size [66, 67]. Figure 5(b) shows the average structure factor for the microemulsion sample. The peak at approximately q~0.25 nm−1 in the S(q) curve indicates the correlation distance between the oil droplets. Besides, the other peaks at around q~0.50 and 0.78 nm−1 show that the average interaction potential distance between the oil droplets was approximately 12±0.01 nm.


## 3.7. DSC Analysis

The thermal behaviors of water in the microemulsion system were investigated by DSC and compared with that of pure water (Figure6). Initially, the samples were cooled to −50°C and then heated to 60°C at a rate of 10°C/min. The thermal transition for the propylene glycol sample was not observed in the temperature range. Pure water showed an endothermic transition near 0°C and enthalpy of fusion of approximately 330 J/g [68]. Pure babassu oil showed two endothermic peaks at 7 and 25°C that were related to the melting temperatures of fatty acid components [69]. The multiple peaks that were observed for the oil sample are due to the polymorphism of different fatty acid. Most vegetable oils can be found in at least three crystalline forms, designated as α, β′, and β [70, 71]. The enthalpy of fusion of pure oil is approximately 100±0.02 J/g, whereas the microemulsion sample showed a broad endothermic melt (fusion) transition in two temperature regions at 11 and 18°C (Figure 6, inset). It is essential to note that the presence of a nearby surface alters the thermodynamic properties, such as freezing point, melting point, and enthalpy [72, 73]. The endothermic transition was not observed at the same position as pure water. This effect is related to the partial miscibility of the surfactants and propylene glycol with water [74]. The literature has shown that the melting temperature deviation in relation to pure water depends on the nature of the interaction between the surfactant and water [72, 73, 75]. When the surfactant concentration is low, the transition of pure water is observed at approximately 0°C. Therefore, when we are dealing with systems in which the interactions depend on the concentrations, the surfactant properties can strongly contribute to the melting temperature and fusion enthalpy. For this reason, the microemulsion enthalpy decreased by 9.5±0.2 J/g when compared with water and pure oil.Figure 6
DSC thermographs for the babassu microemulsion, showing a broad endothermic melt transition in two temperature regions (inset).The presence of two melting peaks is a characteristic behavior of a bicontinuous microemulsion in situations where the oil phase is composed of a single fatty acid [60, 76–78]. However, when the oil phase consists of a triglyceride molecule, which is composed of various fatty acids, this behavior depends on the interactions of each fatty acid with the surfactants species. The first peak in the DSC thermogram of the microemulsion (~11°C) is related to the amount of free water. The second peak (~18°C) refers to the fusion of the oil phase in microemulsion. This thermal transition is shifted to lower temperatures because the fatty acids interact with the surfactant through the nonpolar head.


## 3.8. Rheological Behavior of the System

The rheological behavior of the babassu microemulsion is demonstrated in SM (FigureS5). The shear stress is proportional to increased shear rate, which is characteristic of Newtonian behavior. The profile of shear stress versus shear rate for Newtonian liquids yields a straight line, which is the expected behavior of microemulsions [79–81]. The average viscosity value for the babassu microemulsion was approximately 0.29±0.1 Pas.


## 3.9. Topical Anti-Inflammatory Activity of Babassu Microemulsion

The topical anti-inflammatory activity of the babassu microemulsion compared with pure babassu oil is shown in Figure7. Pure babassu oil and 12.2% babassu oil diluted in acetone inhibited the PMA-induced ear edema by 58.0% (p<0.001) and 17.5% (not significant), respectively. The babassu microemulsion inhibited ear edema by 66.2% (p<0.001), showing an enhanced activity promoted by the microemulsification of babassu oil, since babassu oil at 12.2% (final concentration in the microemulsion) did not have a significant effect on this experimental model. Dexamethasone (0.1 mg/ear) inhibited PMA-induced ear edema by 78.4% (p<0.001).Figure 7
Topical anti-inflammatory activity of babassu oil and microemulsion in PMA-induced ear edema. Results are expressed as the mean ± SEM and analyzed by ANOVA followed by Tukey’s test withp set at 0.05. Different letters show statistical differences between groups (p<0.05). (−) Negative control (acetone); (V) microemulsion vehicle (48.8% surfactants, 39% aqueous phase, and 12.2% water); babassu oil (μL/ear); (ME) babassu microemulsion (μL/ear); and (Dexa) dexamethasone (mg/ear).Skin permeation enhancement by microemulsions has been widely studied for several anti-inflammatory drugs, for example, indomethacin, aspirin, and rofecoxib [82–84]. Microemulsification of babassu oil seems to enhance skin permeation of anti-inflammatory active compounds found in the oil, reaching the same percentages of edema inhibition as pure babassu oil with a much lower oil concentration.


## 4. Conclusions

In this work, we determined the chemical composition of the main fatty acids of babassu oil using GC-MS and the results were confirmed by1H and 13C NMR spectra. Moreover, 13C NMR provided additional information, showing that triacylglycerol was the only positional fatty acid isomer in the glycerol backbone.Babassu oil and lauric showed topical anti-inflammatory activity in different phlogistic agents-induced ear edema in mice, probably due to inhibition of AA metabolism and prostaglandin biosynthesis and/or action, release of histamine and serotonin, and inhibition of preformed cytokine release.The developed babassu nanosystem was characterized as a phase transition microemulsion, which can be considered similarly bicontinuous as an o/w phase in contrast with classical microemulsion systems. Addition of more aqueous phase should form a well-defined o/w microemulsion.The babassu oil microemulsion obtained here displayed advantages due to the combined features of bicontinuous and o/w microemulsions, including very low interfacial tension, high fluctuating interface, and high solubilizing properties. This system may have the ability to incorporate hydrophilic and/or lipophilic drugs that could be released faster than globular microemulsions with superior stability against the aqueous biological environment.Topical anti-inflammatory activity of babassu oil was enhanced by microemulsification, reaching the same ear edema inhibition as pure babassu oil at a much lower concentration. This synthesized nanocarrier represents a new promising strategy for diseases treatment because babassu oil contains fatty acids with important biological properties, such as antioxidant, anti-inflammatory, antitumor, and antimicrobial.



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*Source: 3647801-2017-12-21.xml*