# 18F-Labeling Using Click Cycloadditions

Authors: Kathrin Kettenbach; Hanno Schieferstein; Tobias L. Ross
Journal: BioMed Research International
 (2014)

---

## Abstract

Due to expanding applications of positron emission tomography (PET) there is a demand for developing new techniques to introduce fluorine-18 (t
1
/
2
=
109.8 min). Considering that most novel PET tracers are sensitive biomolecules and that direct introduction of fluorine-18 often needs harsh conditions, the insertion of 18F in those molecules poses an exceeding challenge. Two major challenges during 18F-labeling are a regioselective introduction and a fast and high yielding way under mild conditions. Furthermore, attention has to be paid to functionalities, which are usually present in complex structures of the target molecule. The Cu-catalyzed azide-alkyne cycloaddition (CuAAC) and several copper-free click reactions represent such methods for radiolabeling of sensitive molecules under the above-mentioned criteria. This minireview will provide a quick overview about the development of novel 18F-labeled prosthetic groups for click cycloadditions and will summarize recent trends in copper-catalyzed and copper-free click 18F-cycloadditions.


---

## Body

## 1. Introduction

For the application in positron emission tomography (PET) [1], fluorine-18 provides ideal nuclear physical characteristics forin vivo imaging. Fluorine-18 offers a half-life of 110 min, a β
+-branch of 97%, and especially a low β
+-energy of 635 keV, which is responsible for a very high spatial resolution [2]. The challenges for researchers are to develop convenient 18F-labeling strategies, which include short reaction times and applicability for sensitive biomolecules. Especially the harsh conditions during direct 18F-labeling pose an exceeding challenge [3, 4]. Therefore, most of the radiolabeling strategies focus on 18F-containing prosthetic groups, which allow a sensitive and bioorthogonal 18F-labeling to treat the multitude of functional groups in those bioactive compounds with respect.The most established method, which fulfills all mentioned criteria, is given by click reactions. Especially the Cu(I)-catalyzed variant of the Huisgen 1,3-dipolar cycloaddition of terminal alkynes and azides offers a very powerful reaction with high specificity and excellent yields under mild conditions [5]. As a result, numerous PET tracers have been synthesized using CuAAC in a widespread spectrum of structural varieties of the prosthetic group within the last decade. One of the latest investigations deals with a polar clickable amino acid-based prosthetic group to further improve the pharmacokinetic properties of radiotracers, particularly suitable for peptides and proteins [6].However, the need of cytotoxic copper during CuAAC has led to the necessity of alternative fast and copper-free click reaction strategies for radiofluorination and additionally enabling pretargeting approaches in living systems. Those so-called strain-promoted click reactions can be carried out between cyclooctyne derivatives and azides (strain-promoted azide-alkyne cycloaddition, SPAAC) [7–13] or tetrazines (tetrazine-trans-cyclooctyne (TTCO) ligation) [14–17] as well as between norbornene derivatives and tetrazines [18]. Especially, the TTCO ligation showed promising reaction rates, which makes this click reaction concept very suitable for 18F-labeling and also forin vivo application in living systems. Very recently, new versions of 18F-click cycloadditions are added to the range of reactions [19–25]. In this line, the first 18F-labeled β-lactame became available via a newradio-Kinugasa reaction [21].As a consequence, click cycloaddition is one of the most frequently applied methods for18F-labeling of new bioactive compounds, with or without a catalytic system. This can be impressively illustrated by the fact that over 50 original papers have been published in this research area within the last eight years.Tables1–3 give an overview of the 18F-prosthetic groups, the reaction conditions and reaction partners applied for copper-catalyzed, copper-free and other kinds of 18F-click cycloadditions, respectively. The most important structures of those prosthetic groups are shown in Figures 1, 3, and 5.Table 1
Summary of the prosthetic groups, reaction conditions, and reaction partners applied for copper-catalyzed click18F-fluorination.
18F-prosthetic group
Steps/reaction time1
RCY2
Reacting agent
Catalytic system
Overall reaction time1 (CCA)
RCY2 CCA
Literature
[18
F]
fluoroalkynes
1 step, 10 min
36–81%
N-(3-azidopropionyl) peptides
CuI/NaAsc/DIPEA
30 min
54–99%
[26]
4-[
18F]fluoro-1-butyne
1 step, 15 min (estimated)
n.d.
Glucopyranosyl azide
75–80 min
30%
[27]
4-[
18F]Fluoro-1-butyne
1 step, 15 min
45
±
3
%
2,3,4,6-tetra-O-acetyl-b-D-glucopyranosyl azide
Cu(I)/Asc/2,6-lutidine
30 min
27
±
6
%
[28]
5-[
18F]fluoro-1-pentyne
1 step, 15 min
59
±
6
%
52
±
5
%
1 step, 22 min
86
±
2
%
α
V
β
6
specific peptide A20FMDV2 azide
CuI/Asc
66 min
8.7
±
2.3
%
[29]
6-[
18F]fluoro-1-hexyne
1 step, 12 min
70–85%
γ-(4-azido-butyl)-folic acid amide
CuI
1.5 h
25–35%
[30]
[18
F] fluoroethyl azide
([
18
F]
FEA)
1 step, 15 min
55%
Terminal alkynes
Excess of Cu2+/Asc or copper powder
1 h
61–98% respectively15–98% with copper powder
[31][32]
n.d.
Caspase3
/
7 Selective Isatin
CuSO4/Asc
n.d.
65
±
6
%
[33]
RGD peptides
Cu2+/Asc
47
±
8
%
[34]
3-Cyanoquinoline core
3 h
37
±
3.6
%
[35]
Apoptosis marker ICMT11
CuSO4/Asc/BPDS
n.d.
1–3.4% n.d.c.
[36]
5-Ethynyl-2′-deoxyuridine
CuI/ascorbic acid/DIPEA
75
±
10
%
[37]
[Tyr3
]octreotate analogues
CuSO4/Asc/BPDS
30 min (estimated)
40–64%
[38]
ICMT-11 (automated synthesis)
90 min
3
±
2.6
% n.d.c.
[39]
Nucleosides
CuSO4/Asc
n.d.
8–12% n.d.c.
[40]
4-(prop-2-ynyloxy)Benzaldehyde
35 min
90%
[41]
Haloethylsulfoxides
CuI/ascorbate/DIPEA
n.d.
28.5
±
2.5
%
[42]
50% n.d.c.
Nitroaromatic substrates
CuSO4/Asc
1 h
[43]
71
±
4
%
RGDfK
60 min
60
±
2
%
[44]
55%
Alkyne-func.6-halopurines
One-pot BPDS-copper(I) (CuSO4/NaAsc.)
1 h
55–75%
[45]
n.d.
tert-butyl ester of N-Boc-(S)-propargyl glycine
CuSO4, NaAsc
2.5 h
58
±
4
%
[46]
Precursor: 2 steps
[
18F]FEA: 15 min.
n.d.
3-Butynyl triphenyl phosphonium bromide
1 h
n.d.
[47]
1 step, 5–10 min
68–75%
Alkynes of benzene rings
30 min
25–87%
[48]
[18F] FEA from a polyflourinated sulfonate precursor
n.d.
n.d.
FtRGD
70–75 min
10–30% n.d.c.
[49]
18
F-Fluoro-PEG-Alkyne
1 step, 20 min
85–94%
Various azides
CuSO4/Asc
10–30 min
71–99%
[50]
1 step, 15 min
65
±
1.9
%
E(RGDyK)2 azide
110 min (estimated)
52
±
8.3
%
[51]
57%
Nanoparticle azide
1 h (estimated)
58%
[52]
[18F]PEG3-azide
1 step, 40 min
62
±
4
%
N-alkynylated peptide
CuSO4/Asc/BPDS
2 h (estimated)
31
±
6
%
[53]
n.d.
ZnO nanoparticle alkynes
n.d.
>95%
[54]
[18F]PEG-azide
Precursor: 2 stepslabeling: 1 step
labeling: 58%
γ-(11-azido-3,6,9-trioxaundecanyl)folic acid amide
CuAcetate, NaAsc
2.5 h
8.5%
[55]
4-
[18F]
fluoro-N-methyl-N-(prop-2-ynyl)-
benzenesulfonamide (p
[18F]
F-SA)
Precursor: 3 steps,labeling: 1 step, 80 min
32
±
5
%
Azide-functionalized neurotensin
Cu(I)-TBTA
n.d.
66%
[56]
Azide-functionalized human serum albumin (HSA)
100 min
55–60%
[57]
n.d.
Azide-functionalized phosphopeptide, protein (HAS), oligonucleotide (L-RNA)
CuSO4/Asc
2 h
77%/55–60%/25%
[58]
[18F]
FPy5yne
1 step, 15 min
42%
N3–(CH2)4–CO–YKRI–OH (BG142)
Tetrakis(acetonitrilo) copper(I) hexa fluorophosphates/TBTA
160 min
18.7%
[59]
Azide-functionalized DNA
CuBr/TBTA and 2,6-lutidine
276 min
24.6
±
0.5
%
2-[
18F]fluoro-3-pent-4-yn-1-yloxypyridine ([
18F]FPyKYNE)
20–25 min
20–35%
Azide-functionalized RGD peptide
CuSO4/Asc
125 min
12–18%
[60]
6-[
18F]fluoro-2-etynylpyridine
1 step, 10 min
27.5
±
6.6
%
D-amino acid analogue of WT-pHLIP azide
Cu-Acetate/NaAsc
85 min
5–20%
[61]
propargyl 4-
[18F]
fluorobenzoate
([18F]
PFB)
Precursor: 2 steps,labeling: 1 steps, 15 min
58
±
31
%
Benzyl azide, two lysine derivatives, transglutaminase-reactive peptide
CuSO4/Asc
1 h (estimated)
88
±
4
%,
79
±
33
% and 75
±
5
%
37
±
31
%
[62]
4-[
18F]fluoro-3-nitro-N-2-propyn-1-yl-benzamide([18F] FNPB)
1 step, 40 min
58%
Azido-peptides cRGDfK and D4 peptide
1 h
87–93%
[63]
1-(azidomethyl)-4-
[18F]
-fluorobenzene
4 steps, 75 min
34%
4-Ethynyl-L-phenylalanine-peptide
CuI/NaAsc/DIEA
90 min
90%
[64]
4 steps, 75 min
41%
siRNA alkyne
CuSO4/Asc/TBTA
120 min
15
±
5
%
[65]
1 step, 45 min
84%
siRNA-linker (two new alkyne-bearing linkers)
CuSO4/Asc
120 min
12%
[66]
1-Azido-4-(3-[18F] fluoropropoxy)benzene
4 steps, 75 min
35%
120 min
15
±
5
%
[65]
[18F] (azidomethyl)fluorobenzene
1 step, 94–188 s
around 40%
siRNA alkyne
n.d.
n.d.
[67]
4-[
18F]Fluorophenylazide
around 15%
3,4,6-tri-O-acetyl-2-deoxy-2-
[18F]
fluorogluco-pyranosyl azide
1 step, 30 min
71
±
10
%
Fmoc-L-propargylglycine
CuSO4/Asc
1.5 h (estimated)
60%
[68]
2 step, 7.5 min
n.d.
Alkyne-functionalized peptides (RDG, neurotensin peptoid)
75 min
17–20% n.d.c.
[69]
52%
folate alkyne
Cu-Acetate/NaAsc
3 h
5–25%
[70]
1 step, 10 min
84%
RGD-peptide alkyne
CuSO4/Asc
70–75 min
16–24%
[71]
1 step
1.3–4.7%
Alkyne-bearing protein
CuBr/TTMA
80–100 min
4.1%
[72]
n.d.
ETAR ligand alkyne
CuSO4/Asc
70 min
20–25% n.d.c.
[73]
cyanoquinoline (EGFR) alkyne
90 min
8.6
±
2.3
% n.d.c.
[74]
[18F]
A
r
B
F
3
-
1 step, 20 min
n.d.
Alkyne-functionalized RGD
CuI/Asc
1 h
n.d.
[75]
Alkyne-functionalized bombesin (BBN)
20
±
10
% n.d.c.
[76]
2 steps,
Alkyne-functionalized RGD-boronate
30 min
15–30%
[77]
piperazine-based
[18F]
AFP
[18F]
BFP
AFP: 4 steps, 54 hBFP: 4 steps, 72 h
[
18F]AFP: 1 step, 40 min
[
18F]BFP: 1 step, 40 min
[
18F]AFP: 29
±
5
%
[
18F]BFP: 31
±
9
%
N-Fmoc-e-azido-Lnorleucine (amino acid), SNEW peptide
CuSO4, Asc
2 h
Amino acid: 59–79%SNEW peptide: 17–25%
[78]
[18F]
serine
2 steps, 125 min
28
±
5
%
cRDG-azide
CuSO4, Asc
145 min
75%
[6]
1Calculated as sum from all steps, for the 18F-prosthetic group, respectively, for the overall reaction yielding the click product, starting from fluorine-18.
2Radiochemical yields for the 18F-prosthetic group starting from fluorine-18 for the click reaction, respectively; decay corrected, as long as not noted elsewise.
CCA: click cycloaddition; (n.)d.c.: (not) decay corrected; Asc: ascorbate; DIPEA: diisopropylethylamin; TBTA: tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine; n.d.: no data.Table 2
Summary of the prosthetic groups, reaction conditions, and reaction partners applied for copper-free click fluorination.
18F-prosthetic group
Steps/reaction time1
RCY2
Reacting agent
Reaction type/catalytic system
Overall reaction time1 (CCA)
RCY2 CCA
Literature
[18F]COT
1 step, 15 min
71%
3,6-diaryl-s-tetrazine
inverse electron-demand DA cyclo-addition
30 min (without HPLC)
>98%
[14]
[18F]FB-DBCO
1 step, 60 min
85%
Various azides
Strain-promoted click 1,3-dipolar cycloaddition
2 h
69–98%
[7]
TCO-derivative:Aza-DBCO-BN(bombesin)
9 steps, —
17%
Three different[
18F]azides
30 min (without HPLC)
19–37%(depending on azide)
[8]
[18F]DBCO
1 step, 1 h
21%
Tyr3-octreotide-N3(TATE)
1.5 h
95%
[9]
[18F]TCO
[14]
[14]
Tetrazine-RGD
Inverse electron-demand DA cyclo-addition
30 min
90%
[15]
[18F]bifunctional azadibenzocyclo-octyne
1 step, 30 min
24.5%
Alkyl azide
Strain-promoted click 1,3-dipolar cycloaddition
202
±
34 min
74
±
4.8
%
[10]
[18F]PEG4 azide
1 step, 45 min
63%
cRGD-DBCO
80 min
92%
[11]
[18F]cyclooctyne
6–11 steps,30–80 h (depending on the derivative)
20–57% (depending on the derivative)
[
18F]2-fluoro-ethylazide
30 min.
9.6–97% (depending on COT and solvent)
[12][79]
[18F]trans-cyclooctene ([
18F]TCO)
1 step, 102 min
46.1
±
12.2
%
Tetrazine modified exendin-4
Inverse electron-demand DA cycloaddition
3 h
46.7
±
17.3
%
[16]
Polymer modified tetrazine
89.2%in vivo
[80]
[18F]amine-functionalised norbornene
1 step, 52 min
60
±
17
%
Tetrazine(peptide-/bombesin-derivatives)
82 min (without preparation of[
18F]SFB)
46–97% (depending on the tetrazine)
[18]
[18F]FBA-C6-DBCO
[10]
[10]
α
V
β
6-specific peptide
Strain-promoted click 1,3-dipolar cycloaddition
click:40
±
4 min
11.9
±
3.2
%
[13]
1Calculated as sum from all steps, for the 18F-prosthetic group, respectively, for the overall reaction leading to the click product, starting from fluorine-18.
2Radiochemical yields for the 18F-prosthetic group starting from fluorine-18 for the click reaction, respectively; decay corrected, as long as not noted elsewise.
CCA: click cycloaddition; DA: Diels Alder; DBCO:aza-dibenzocyclooctyne; TCO: trans-cyclooctyne.Table 3
New developments in18F-click [3+2]cycloadditions, showing the 1,3-dipolar 18F-prosthetic groups, reaction type, and conditions.
18F-prosthetic group
Steps/reaction time
RCY
Reacting agent
Reaction type/catalytic system
Overall reaction time1 (CCA)
RCYCCA
Literature
C-(4-[
18F]fluoro-phenyl)-N-phenyl-nitrone
2 steps/20 min, (labeling of[
18F]FB-CHO: 1 step, 50 min)
22–37%1 ([
18F]FB-CHO: 30–50%)(18F-nitrone: 74%)
Various maleimides
80 min(10 min)
87–91%
[19]
4-[
18F]fluoro-benzonitrile oxide
3 steps/20 min (labeling of[
18F]FB-CHO: 1 step, 50 min)
28–46%1 ([
18F]FB-CHO: 30–50%)(18F-nitro oxide: 92%)
Various dipolarophiles
1,3-dipolar[3+2]cycloaddition, no catalyst
80 min(10 min)
36–99%
[20]
Cyclononyne-indomethacins(COX-2 inhibitor)
81%
Maleimide-indomethacins(COX-2 inhibitor)
55%
Propyne-indomethacins(COX-2 inhibitor)
35%
N-hydroxy-4-[
18F]fluorobenz-imidoyl chloride
4 steps/20 min (labeling of[
18F]FB-CHO: 1 step, 50 min)
27–45%1 ([
18F]FB-CHO: 30–50%)(18F-nitro oxide: 92%)(18F-benzimidoyl Cl: 99%)
Cyclononyne-β-Ala-Phe-OMe(dipeptide)
85 min(10 min)
88%2
Norbornene-β-Ala-Phe-OMe(dipeptide)
82%2
C-(4-[
18F]fluoro-phenyl)-N-phenyl-nitrone
2 steps/20 min, (labeling of[
18F]FB-CHO: 1 step, 50 min)
22–37%1 ([
18F]FB-CHO: 30–50%)(18F-nitrone: 74%)
Terminal alkynesmethyl propiolate
radio-Kinugasa, CuSO4, AscONa (L-histidine)
80 min(10 min)
89%(trans/cis = 2 : 3)
[21]
Terminal alkynepropargyl alcohol
radio-Kinugasa, CuI (CuI-stabilizing ligands or pyridine)
100 min(30 min)
82%(trans/cis = 1 : 5)60%(trans/cis = 1 : 5)
Terminal alkyne1-propargyl uracyl(nucleobase chimera)
65%(trans/cis = 4 : 1)
propiolyl-β-Ala-Phe-OMe(dipeptide)
radio-Kinugasa, CuSO4, AscONa(L-histidine)
80 min(10 min)
85%(trans/cis = 1 : 3)
propiolated protein(BSA)
32%
o-/p-[
18F]fluoro-phenyl acetylene
n.d.
n.d.
3,6-dihydro-2H-1,4-oxazine-4-oxide
radio-Kinugasa, CuI (1,10-phenanthroline)
(10 min)
52% (ortho)41% (para)
1Calculated as sum from all steps.
2Best RCY, obtained only with high precursor amounts.
FB-CHO: 4-fluorobenzaldehyde; CCA: click cycloaddition; PHA:N-phenylhydroxylamine; AscONa: sodium ascorbate’; BSA: bovine serum albumin; n.d.: no data.Figure 1
Lead structures of the most important18F-prosthetic groups applied for copper-catalyzed click 18F-fluorination.


## 2. Copper-Catalyzed18
F-Click Cycloadditions

In the last decade, the copper-catalyzed azide alkyne cycloaddition (CuAAC), which has first been reported independently by Rostovtsev et al. [81] and Tornøe et al. [82] in 2002, has spread over almost all fields of chemistry [83–87], biology [88–90], and material science [91, 92]. The great advantage of this method is given by its outstanding efficiency, its regiospecificity, and fast formation of 1,4-disubstituted 1,2,3-triazoles at ambient temperatures, which is particularly suitable for 18F-labeling of sensitive biomolecules. In particular, the CuAAC enables incorporation of fluorine-18 via a prosthetic group under mild and bioorthogonal conditions [22–25]. 1,2,3-triazoles were first introduced by Michael, who described the formation of a 1,2,3-triazole from a phenylazide in 1893 [93]. Following this pioneering work, Dimroth, Fester, and Huisgen described this type of reaction as a 1,3-dipolar cycloaddition for the first time in 1963 [5].In 2006, Marik and Sutcliffe published the application of the CuAAC as an18F-labeling strategy for the first time [26]. They radiolabeled three different alkyne precursors in radiochemical yields (RCY) of 36–81%. Afterwards they were reacted them with azido-functionalized peptides in RCY of 54–99% and an overall reaction time of 30 min. Thus, they could show a new, very fast, efficient, and mild 18F-labeling strategy for complex compounds, especially appropriate for sensitive biomolecules. Only two years later, the suitability of this approach was demonstrated for the 18F-labeling of a folate derivative forin vivo tumor imaging with the same prosthetic group, 6-[18F]fluoro-1-hexyne [30]. The radiofolate was obtained in RCY of 25–35% and was applied to KB-tumor bearing mice. A specific tumor accumulation could be observed by using the folate receptor (FR) targeting concept. Furthermore, Kim et al. used 18F-labeled alkynes as prosthetic groups for the 18F-labeling of 2,3,4,6-tetra-O-acetyl-β-D-glucopyranosyl azide [27], which in turn was employed to label the α
V
β
6 specific peptide A20FMDV2 [28].Considering all known clickable prosthetic groups for18F-labeling, [18F]fluoroethyl azide ([18F]FEA) is certainly one of the most investigated clickable 18F-prosthetic groups. Until today, about twenty different manuscripts deal with [18F]FEA to radiolabel a broad variety of biomolecules and compounds. In 2007, Glaser and Årstad [31] mentioned for the first time the preparation of [18F]FEA with a RCY of 55% using 2-azidoethyl-4-toluenesulfonate as precursor. As a proof of concept, they reacted [18F]FEA with different terminal alkynes in very good to excellent RCY of 61–98%. With respect to the catalytic system copper sulfate in combination with ascorbic acid or sodium ascorbate has mainly been used, whereas only in a few approaches copper(I) iodide was used [37, 42]. It has been shown that addition of bathophenanthroline disulfonate (Cu
I stabilizing agent) accelerates the 1,3-dipolar cycloaddition [36, 38, 45]. The very good access to [18F]FEA led to the development of a variety of radiotracers labeled with this prosthetic group, like 18F-deoxyuridine [37], 18F-fluoro-oxothymidine (18F-FOT), or 18F-fluoro-thiothymidine (18F-FTT) [40] as well as apoptosis markers [36] and several peptide systems [34, 44, 49]. In 2012, Smith et al. [40] described the reduction of [18F]FEA using copper wire under acidic conditions, which is a possible explanation of the poor yields during some click reactions.In 2007, Sirion et al. [50] reported for the first time [18F]fluoro-PEGx-derivatives (x = various polyethylene glycol (PEG) ratios) as new 18F-labeled prosthetic click groups. These compounds showed a reduced volatility and increased polarity compared with other 18F-labeled prosthetic groups like [18F]FEA or [18F]fluoroalkynes. These properties ease their handling as well as improving thein vivo behavior of the labeled compounds. The compounds showed a longer circulation time and a reduced renal clearance making them very suitable forin vivo application. Sirion et al. described the preparation of different aliphatic and aromatic 18F-PEG-azides and 18F-labeled alkynes in RCY of 85–94%. As a proof of concept, they carried out cycloadditions with the 18F-labeled prosthetic groups and the corresponding alkynes, respectively, azides in high RCY of 71–99%. Several other groups continued this work by using the 18F-labeled PEGylated prosthetic groups for labeling cRGD derivatives [51] and other peptides [53], nanoparticles [52, 54], or folates [55].To increase the lipophilicity and metabolic stability of radiotracers, [18F]fluoro-aryl-based prosthetic groups have been developed and investigated. In 2007, Ramenda et al. [56] published for the first time a 4-[18F]fluoro-N-methyl-N-(prop-2-ynyl)-benzenesulfonamide (p-[18F]F-SA), which was obtained in RCY of 32 ± 5%. Subsequently, this prosthetic group was used for radiolabeling an azido-functionalized neurotensin giving a RCY of 66%. Furthermore, the same group used the 18F-aryl prosthetic group for the labeling of human serum albumin (HSA) [57] and other proteins, phosphopeptides, andL-RNA [58] in good RCY. A pyridine-based 18F-prosthetic group was first introduced by Inkster et al. [59] in 2008 by reacting [18F]FPy5yne with a model peptide in RCY of 18.7% and an overall reaction time of 160 min. They started from either 2-nitro- or 2-trimethylammonium pyridine to synthesize [18F]FPy5yne with a RCY of 42%. Furthermore, [18F]pyridine derivatives have been used to radiolabel cRGDs [60] and theD-amino acid analog of WT-pHLIP [61].In 2009, Vaidyanathan et al. [62] presented a prosthetic group based on a 4-[18F]fluorobenzoate. Propargyl-4-[18F]fluorobenzoate ([18F]PFB), which could be obtained in RCY of 58
±
31% within 15 min. To investigate the labeling properties of this new prosthetic group, numerous compounds have been 18F-labeled using [18F]PFB with RCY from 37% to 88% and overall reaction times of about 1 h. Another approach was published by Li et al. in 2012 [63], who synthesized 4-[18F]fluoro-3-nitro-N-2-propyn-1-yl-benzamide ([18F]FNPB) for 18F-labeling of cRGDfK and a D4 peptide, which was identified as an EGFR targeting ligand. This approach was followed by the synthesis of 1-(azidomethyl)-4-[18F]fluorobenzene by Thonon et al. [64]. They did a multistep radiosynthesis (4 steps), where the fluorine-18 was introduced in the first step. The desired radiolabeled product could be obtained in a RCY of 34% within 75 min and was used itself to label a 4-ethynyl-L-phenylalanine-containing peptide. The same prosthetic group was also employed by Mercier et al. [65] and Flagothier et al. [66] for 18F-labeling ofsiRNA. Other structural analog prosthetic groups have also been developed by Mercier et al. [65] and Chun and Pike [67].To improve thein vivobehavior of peptides with respect to blood clearance and stability, Maschauer and Prante developed 18F-gluco-derivatives for CuAAC-radiolabeling of Fmoc-L-propargylglycine with a RCY of 60% [68]. They showed that the 18F-click labeling reaction was more convenient by using the β-anomeric derivative of the azides, respectively, alkynes, giving very high RCY of 71
±
10%. One year later, they published the firstin vivo evaluation of an 18F-labeled RGD peptide labeled with [18F]FDG-β-Az in U87MG-tumor bearing mice showing an improved blood clearance and stability [65, 66]. Likewise, Fischer et al. demonstrated in 2012 that a [18F]fluorodeoxyglycosyl folate could be obtained in RCY of 5–25% and subsequent biodistribution and PET-imaging studies showed a high and specific uptake of the radiotracer in FR-positive tumors [70]. The variety of new 18F-labeling strategies using 18F-Fluoroglycosylation is the focus of a review article as a part of this special issue provided by Maschauer and Prante [94].As another promising approach, Li et al. presented in 2013 an alkyne-functionalized aryltri-[18F]fluoroborate for radiolabeling azido-bombesin and azido-RGD. The major advantage of this method is the two-step, one-pot procedure providing a water-soluble and noncoordinating aryltri-[18F]fluoroborate anion, which provided specific activities up to 555 GBq/μmol [75, 76, 95].Two new piperazine-based prosthetic groups, 1-(but-3-ynyl)-4-(3-[18F]fluoropropyl)piperazine ([18F]BFP) and 1-(3-azidopropyl)-4-(3-[18F]fluoropropyl)piperazine ([18F]AFP), have recently been developed by Pretze and Mamat [78]. Spiro salts were used as precursors, facilitating purification by using solid phase extractions (RP-18 or SiO2-cartridges). Both prosthetic groups could be obtained in RCY of about 30% using an automated synthesis module. To avoid Glaser coupling, which has been observed by using [18F]BFP for radiolabeling of peptides, [18F]AFP was used instead. An important observation was the fact that the applied peptide formed very strong complexes with the copper catalyst, which required the use of bispidine as a strong chelating agent to remove cytotoxic copper species.One of the latest developments describes the synthesis of an18F-labeled alanine derivative as a new prosthetic click group, reported by Schieferstein and Ross [6]. In this case, an amino acid-based prosthetic group has been developed to improve the pharmacokinetic profile of 18F-click-labeled biomolecules. The prosthetic group was obtained in good RCY of 28
±
5% from a two-step reaction as described in Figure 2. The final 18F-labeled prosthetic group was subsequently reacted with an azido-RGD as model system in RCY of 75% within 20 min.Figure 2
Radiosynthesis of a new amino-acid based18F-prosthetic group (N-propargyl-2-amino-3-[18F]fluoro-propionic acid, “[18F]serine”) for 18F-CuAAC-labeling of complex biomolecules. (i) [K
⊂
2.2
.
2]+/F
18
-, DMSO, 140°C, 10 min; (ii) hydrochloric acid (3.3 M), 100°C, 15 min; for analytical purposes (sequential deprotection): (iii) sodium hydroxide (3.3 M), 60°C, 5 min; (iv) hydrochloric acid (3.3 M), 100°C, 15 min.Figure 3
Lead structures of the most important18F-prosthetic groups applied for copper-free click 18F-fluorination.Considering the above-mentioned prosthetic groups for radiolabeling with fluorine-18, Table1 summarizes important properties of those components. It has been shown that the integration of an 18F-propyl, 18F-ethyl, or 18F-aryl moiety can provide an improved metabolic profile and that the glycosylation or PEGylation can further improve thein vivo behavior. Furthermore, forin vivo application a total removal of the copper catalyst is essential. This could be very challenging in the case where peptides or proteins are able to complex copper species from the catalytic system.


## 3. Copper-Free18F-Click Cycloadditions

Even though a large number of novel radiotracers using click chemistry have been developed, none of them has entered clinical routine to date, apart from18F-RGD-K5, which is already used in clinical trials in US. This can be explained by the need of cytotoxic copper during radiotracer syntheses by using copper-catalyzed 1,3-dipolar Huisgen cycloadditions [96]. Thus, there is still a demand for facile (metal-free) and robust 18F-labeling reactions for the syntheses of radiotracers for imaging of malignanciesin vivo. This leads to the development of catalyst-free click-labeling approaches, which spare copper species during labeling steps and even enablein vivo pretargeting concept. Recent developments deal with biocompatible strain-promoted copper-free versions of the alkyne-azide cycloaddition (SPAAC), where the focus has been set on derivatives of cyclooctynes and dibenzocyclooctynes. First approaches focus on the reaction of 18F-labeled cyclooctynes with azide-bearing biomolecules. On the other hand, in further approaches cyclooctyne-carrying bioactive compounds are used, which can be labeled with different 18F-labeled azides. In the beginning, only a few studies have been reported due to the complex and low yielding syntheses of strained cyclooctynes [10, 12, 14]. However, nowadays lots of cyclooctyne derivatives are commercially available, which facilitates the precursor syntheses and opens a wide range of applications.In 2011 Bouvet et al. [7] published the first example of a SPAAC with 18F-labeledaza-dibenzocyclooctyne, [18F]FB-DBCO, and a plethora of azides. The 18F-labeled building block was synthesized via acylation of commercially availableN-(3-aminopropionyl)-5,6-dihydro-11,12-didehydrodibenzo[b,f]azocine withN-succinimidyl-4-[18F]fluorobenzoate ([18F]SFB), which can be easily prepared in an automated synthesis module [97]. The 18F-labeled cyclooctyne could be obtained in a RCY of 85% and a purity >95% within 60 min. The evaluation of this building block in healthy Balb/C mice showed 60% of intact compound at 60 min p.i. and had a blood clearance half-life of 53 s. Besides, the compound was stable in methanol and phosphate buffer over 60 min. Subsequently, [18F]FB-DBCO was reacted with various azides as proof of principle showing different structural complexities. In all reactions, the formation of two regioisomers (1,4- and 1,5-triazole) has been observed and in some cases a separation of the regioisomers by HPLC was impossible. All 18F-labeled radiotracers were obtained in good to excellent RCY of 69–98% within an overall reaction time of about 2 h. However, the reaction rates in these cases were much slower compared to other examples of bioorthogonal reactions, limiting this new approach forin vivo pretargeting applications.A cyclooctyne derivative has been conjugated to bombesin (aza-DBCO-BN, 9 steps) with an overall yield of 17% by Campbell-Verduyn et al.   [8]. Theaza-DBCO-BN was reacted with various 18F-azides giving RCY of 19–37% within 30 min. In 2011, Arumugam et al. [9] investigated the direct 18F-labeling of azadibenzocyclooctyne (DBCO) yielding the 18F-labeled prosthetic group (RCY = 36%). The radiolabeling was followed by a click reaction with anazido-octreotide leading to the 18F-labeled octreotide in a RCY of 95% within a total reaction time of 1.5 h. In contrast, other working groups used 18F-cyclooctynes for labeling RDG-derivatives [11] as well as further integrin-specific peptides [10, 13].Another possibility to perform copper-free click reactions is given by the inverse electron demand of the Diels Alder cycloaddition between a cyclooctene and a tetrazine under the release of nitrogen. The so-called tetrazine-trans
-cyclooctene ligation (TTCO ligation) was first published by Li et al. in 2010 [14]. Concerning the instability of the tetrazines, it is more practical to functionalize the biomolecule with a tetrazine followed by the reaction with an 18F-labeled cyclooctene. The latter are much more suitable for direct 18F-labeling than tetrazines. For this purpose a nosylate precursor was used for 18F-labeling of the cyclooctene providing RCY of 71% within 15 min. To investigate the suitability of the 18F-prosthetic group in click reactions, the 18F-cyclooctene was reacted with a 3,6-di(2-pyridyl)-S-tetrazine in an excellent RCY of 98% within 10 s, showing its outstanding feasibility forin vivo pretargeting approaches. These fast reaction rates made this approach very attractive that even 11C-labeling reaction was explored using the inverse electron demand Diels Alder cycloaddition between a cyclooctene and a tetrazine [98]. In 2011, 18F-labeled cyclooctene was linked to a tetrazine-RGD derivative by Selvaraj et al. [15] with a RCY of 90% within 5 min at room temperature. The resulting 18F-labeled tracer was tested inin vivo experiments showing a high tumor accumulation, which could selectively be blocked. In 2012, the group of Devaraj et al. [80] published for the first time thein vivo click reaction of [18F]trans-cyclooctene and a polymer-modified tetrazine (PMT). The radiolabeled peptide 18F-PMT10 could be obtained in a RCY of 89.2%. Whole body animal PET scans were carried out 3 h p.i., showing renal clearance and a widespread tissue distribution as can be seen in Figure 4. Previously, the same group described the synthesis of an 18F-labeled cyclooctene with a RCY of 46.1
±
12.2%. Subsequently, this prosthetic group was clicked with a tetrazine-modified exendin-4 in RCY of 46.7
±
17.3% [16].PET and autoradiography using18F-tetrazine agents. (a) PET/CT fusion of LS174T tumor xenograft labeled using eithertrans-cyclooctene (TCO) monoclonal antibodies (mAb TCO) or control unlabeled antibodies (mAb) followed by 18F-PMT10 (polymer-modified tetrazine). Arrows indicate location of the tumor xenograft. The bladder was omitted for clarity. (b) Imaging using autoradiography (left side) and fluorescence slices after targeting with fluorescence TCO monoclonal antibody and 18F-PMT10. (c) PET/CT fusion of mouse bearing A431 and LS174T tumors after targeting with anti-A33 TCO monoclonal antibodies followed by 18F-PMT10. Arrows indicate location of tumors and the liver was omitted for clarity. (d) Autoradiography of representative 1 mm LS174T and A431 tumor slices after multistep targeting (reprinted with permission from [80]; Copyright 2012 National Academy of Sciences of the United States of America).
(a)
(b)
(c)
(d)Figure 5
Lead structures of new18F-prosthetic groups applied for click 18F-fluorination.A similar strategy was published by Knight et al. in 2013, where an18F-labeled amino-functionalized norbornene was reacted with a tetrazine-modified peptide [18]. The 18F-labeled norbornene was obtained using N-succinimicyl-4-[18F]fluorobenzoate ([18F]SFB) in RCY of 60
±
17% within 52 min. As a proof of concept, two different tetrazines, an asymmetric dipyridyl tetrazine, and a tetrazine-modified bombesin peptide were labeled with 18F-labeled norbornene derivative ([18F]NFB) in 46–97% RCY within 82 min.Considering the copper-free click labeling of bioactive compounds with fluorine-18, both the strain-promoted alkyne-azide cycloaddition (SPAAC) and the tetrazine-trans
-cyclooctyne ligation (TTCO ligation) show promising results. Regardingin vivo pretargeting approaches, only the TTCO ligation showed favorable results and reaction rates, which are suitable for this application [80]. Table 2 summarizes reaction conditions, radiochemical yields, and reaction partners of those components.


## 4. New Developments in18F-Click Cycloadditions

The latest developments in metal-free18F-click cycloadditions have been reported by Zlatopolskiy et al. [19–21] (Table 3). In a first approach, the 18F-labeled building block C-(4-[18F]fluorophenyl)-N-phenyl nitrone was developed to form 18F-isoxazolidines via high-yielding [3+2]cycloadditions with various maleimides [19]. C-(4-[18F]fluorophenyl)-N-phenyl nitrone was obtained from the reaction of 4-[18F]fluorobenzaldehyde and N-phenylhydroxylamine in high RCY of 74% with 10 min. In the subsequent click cycloaddition step, differently substituted maleimides as model dipolarophiles were used to form the corresponding isoxazolidines as endo-/exoisomers in high yields of up to >90% within 10 min. A one-pot strategy within situ generation of C-(4-[18F]fluorophenyl)-N-phenyl nitrone provided the desired 18F-isoxazolidines only in moderate yields of 25% and only after heating to 110°C. Under optimized conditions, 18F-isoxazolidines were obtained from fast 18F-click [3+2]cycloadditions.In further studies, the same group used 4-[18F]fluorobenzonitrile oxide instead of C-(4-[18F]fluorophenyl)-N-phenyl nitrone as 1,3-dipol for milder reaction conditions [20] (Table 3). 4-[18F]fluorobenzonitrile oxide was obtained in 92% RCY within 10 min from the reaction of 4-[18F]fluorobenzaldehyde (RCY: 30–50%, 50 min [99]) with hydroxylamine and subsequent treatment with phenyl iodine bis(trifluoroacetate).After the click [3+2]cycloaddition to various18F-labeled model 2-isoxazolines and isoxazoles was successfully tested, the novel method was applied to three different COX-2 inhibitors (indomethacin conjugates) carrying dipolarophilic moieties of cyclononyne, maleimide, and propyne. The resulting products were obtained in moderate to excellent RCY of 81%, 55%, and 35%, respectively. It is noteworthy that, for the propyne derivative, the milder oxidant [bis(acetoxy)iodo]benzene was used to avoid decomposition. Finally, the method was successfully adapted for 18F-labeling of two model dipeptide conjugates, cyclononyne- and norbornene-β-Ala-Phe-OMe. However, the original cycloaddition using 4-[18F]fluorobenzonitrile oxide did only provide traces of the desired products. Consequently, 4-[18F]fluorobenzonitrile oxide was further treated with chloramine T (CAT)in situ forming the more stable building block N-hydroxy-4-[18F]fluorobenzimidoyl chloride. With the use of high precursor (peptides) amounts, the latter enabled excellent RCY of the 18F-labeled dipeptides of up to 88% within 10 min at room temperature [20]. Under optimized conditions low precursor amounts of 5 nmol (cyclononyne) and 50 nmol (norbornene-β-Ala-Phe-OMe) still allowed RCY of 56% and 47%, respectively.In a very recent report, Zlatopolskiy and coworkers applied their18F-labeled nitrone, C-(4-[18F]fluorophenyl)-N-phenyl nitrone, for the first formation of 18F-labeled β-lactames via the CuI-catalyzed Kinugasa reaction [21] (Table 3). The optimized reactions went smooth under very mild conditions to give the 18F-labeled model β-lactames in high RCY and various isomeric mixtures of thetrans- andcis-product. In dependency on the reactivity of the terminal alkynes, the reaction parameters needed (individual) optimization regarding catalyst system, solvent, temperature, and CuI-stabilizing ligands. As a biologically relevant molecule the 18Flabeled nucleobase chimera was synthesized as potential PET-imaging agent for bacterial infections.Moreover, the dipeptideβ-Ala-Phe-OMe was propiolated and used in this radio-Kinugasa reaction to give excellent RCY of 85% of the 18F-labeled dipeptide under very mild conditions (aqueous solution, room temperature) [21]. Similarly, this new method was successfully transferred to the 18F-labeling of proteins. Bovine serum albumin (BSA) was conjugated with 3-propiolamidopropyl chloroformate. This propiolated BSA was successfully radiolabeled with fluorine-18 in the radio-Kinugasa reaction.


## 5. Conclusions

The field of click cycloadditions had and still has a major impact in18F-labeling chemistry. The very mild reaction conditions mostly applicable and the excellent efficiency of all types of these reactions are particularly suitable for 18F-labeling. Especially, complex and sensitive biomolecules benefit from this methodology. No protection group chemistry is needed and the 18F-click cycloaddition step provides the final radiotracer.Besides several new18F-labeled radiotracers are available via click cycloadditions, and the metal-free versions even enabled pretargeting concepts byin vivo click. The latest development of a radio-Kinugasa reaction towards the first 18F-β-lactames demonstrates the highly active field and the broad applicability of 18F-click cycloadditions.



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*Source: 361329-2014-05-27.xml*