# Nanogravimetric and Optical Characterizations of Thrombin Interaction with a Self-Assembled Thiolated Aptamer

Authors: Jane Politi; Ilaria Rea; Fabrizia Nici; Principia Dardano; Monica Terracciano; Giorgia Oliviero; Nicola Borbone; Gennaro Piccialli; Luca De Stefano
Journal: Journal of Sensors
 (2016)

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## Abstract

Efficient biorecognition of thrombin (TB), a serine protease with crucial role in physiological and pathological blood coagulation, is a hot topic in medical diagnostics. In this work, we investigate the ability of synthetic thrombin aptamer (TBA), immobilized on a gold substrate, to bind thrombin by two different label-free techniques: the quartz crystal microbalance (QCM) and the spectroscopic ellipsometry (SE). By QCM characterization in the range from 20 to 110 nM, we demonstrate high specificity of TBA-TB interaction and determine affinity constant (Kd) of 17.7±0.3 nM, system sensitivity of 0.42±0.03 Hz nM−1, and limit of detection (LOD) of 240±20 pM. The interaction between TBA and TB is also investigated by SE, an all-optical method, by quantifying the thickness increase of the TBA film assembled on gold substrate. AFM characterization of TBA and TB molecules deposited on flat silicon surface is also supplied.


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## Body

## 1. Introduction

Thrombin (TB) is a strong vasoconstrictor involved as main agent in vasospasm [1]. Beyond its key role in the dynamic process of thrombus formation, TB has a pronounced proinflammatory character, which may influence the development and progression of atherosclerosis [2]. Concentration levels of TB in human blood around 5 ÷ 10 nM have been correlated to growth process of tumor cells [3]. It is well known that TB molecules expose two electropositive exosites that specifically interact with substrates: fibrinogen recognition exosite, at the base of the active-site cleft, and heparin recognition exosite [4, 5]; both substrates, fibrinogen and heparin, are molecules of crucial importance in coagulation/anticoagulation mechanisms [6]. Therefore, the specific recognition and quantitative detection of TB blood concentration in the nM range play a crucial role in fundamental research as well as to clinical practice [7].These are the reasons justifying the design and realization of a real-time, highly specific biosensor for thrombin detection in early diagnosis of cardiovascular diseases. The demand of biomolecular probes, alternative to peptides and antibodies in biochemical sensing applications, is nowadays growing in order to avoid time-consuming steps required by labeling and offline analysis.Aptamers are oligonucleotides sequences of DNA or RNA of variable length, generally between 15 and 80 bases that bind a specific target molecule, selected in vitro by systematic evolution of ligands (SELEX) [8]. The SELEX process, a multisteps procedure, selects the oligonucleotide sequences, which will form the aptamer, on the basis of their specific binding affinity to a target analyte, chosen from a library of sequences. By this kind of approach, a large number of aptamers have been discovered for diagnostics, therapeutics, and other biotechnical applications [9]. Aptamers are known for their specificity: on the basis of slight differences, such as the presence or the absence of hydroxyl or methyl groups in chemical structure [10–13], they are able to discriminate between D-enantiomer and L-enantiomer of the target molecules [14]. The central feature of aptamers is the high affinity to targets, which was found comparable to corresponding monoclonal antibodies and characterized by affinity constant values in the picomolar range [10]. Aptamers affinity and specificity properties highlight their huge potential in diagnostic, therapeutic, and (bio)analytical applications [15–17]. The thrombin binding aptamer (TBA) is a classic example of a nucleic acid with potential therapeutic and/or diagnostic ability. TBA adopts a monomolecular chair-like G-quadruplex structure consisting of two G-tetrads connected by two laterals TT and central TGT loops [18]. In presence of thrombin, TBA folds into a specific three-dimensional structure that ensures high thrombin binding affinity, depending on ionic strength, pH, and coadsorbent molecules [19–21].In this work, we studied the interaction between TBA, once immobilized on a gold substrate, and TB by two different label-free methods: quartz crystal microbalance (QCM) and spectroscopic ellipsometry (SE). Quartz crystal microbalance (QCM) based biosensors have received an increasing interest in recent years due to their high sensibility and the ability to perform a real-time monitoring [22–24]. Nanogravimetry exploits the piezoelectric quartz resonator (QR) properties as transducer in quantifying the resonance frequency shift Δf when a mass m is adsorbed to/desorbed from its surface [25]. The results of gravimetric analysis were completed by spectroscopic ellipsometry, a powerful optical technique to investigate optical properties (complex refractive index) and thickness of thin films. Both analytical techniques demonstrated the ability of TBA to selectively bind TB, even if it is covalently bound on a modified gold surface.


## 2. Experimental Section

### 2.1. Aptamer Synthesis and Purification

The thiolated-thrombin binding aptamer (TBA-SH) (4726 Da) was synthesized by Expedite 8909 DNA synthesizer (Applied Biosystems, USA) using standard solid phaseβ-cyanoethylphosphoramidite chemistry on a Controlled Pore Glass (CPG) resin, as previously reported [26]. Last coupling step was performed with a proper thiol-modified phosphoramidite (dithiothreitol group, DTT). All the reagents and the CPG support were purchased from Glen Research. The oligomers were detached from the resin and deprotected by treatment with aqueous ammonia at 55°C, overnight. The combined filtrates and washing were purified through reverse phase HPLC (Purospher STAR, 5 μm, 250 × 10 mm, Merck), to allow the identification of our product as the most retained peak, by virtue of the presence of the trityl group protecting the thiol function. We used a linear gradient from 0% to 100% B for 30′ and flow rate 1.2 mL/min, where A is 0.1 M triethylammonium bicarbonate (TEAB) buffer, pH 7, and B is acetonitrile (ACN).After the purification of our product, the deprotection from the trityl group was performed. To this purpose, the lyophilized oligomer was dissolved in 0.1 M triethylammonium acetate (TEAA) buffer, to have a final concentration of 7 × 10−4M, and treated with 0.15 volumes of the final volume of 1 M aqueous silver nitrate solution. After 30 min of thoroughly mixing, 0.2 volumes of 1 M DTT solution were added. The solution was stirred for 5 min and then centrifuged to remove the silver DTT complex. The supernatant was collected and the precipitate was washed with TEAA 0.1 M and centrifuged for the second time. The combined supernatants were purified by Sep-Pak cartridges (C-18) to remove the excess of DTT. The obtained fractions were collected and dried under vacuum. Samples concentrations were determined in water by measuring the absorbance at 260 nm at 90°C using the nearest-neighbor calculated molar extinction coefficient of d[5′GGTTGGTGTGGTTGG3′] ε = 143,000 M−1cm−1.Finally, the sample was dissolved in PBS buffer (Sigma-Aldrich; 10 mM phosphate buffer, 2.7 mM KCl, 137 mM NaCl, pH 7.4), and the corresponding quadruplex was formed by heating the sample at 90°C and slowly cooling at room temperature.


### 2.2. Thiolated-TBA Self-Assembling

Self-assembling TBA-SH (4904.72 Da) on gold-coated quartz resonator was performed flowing 1μM TBA-SH solution in phosphate buffer 20 mM pH 7.4 for 100 min at room temperature. Three flowing cycles were performed in order to refresh the solution in contact with gold quartz surface.


### 2.3. Quartz Crystal Microbalance and Thrombin Monitoring

The mass variations were measured by a QCM instrument (Novaetech S.r.l., Italy), connected to a computer by a producer-released software. The quartz resonators (QRs) (IEV S.r.l., Italy) are characterized by a fundamental nominal frequency of 10 MHz, a blank diameter of 8 mm, and a gold lamina diameter of 4.5 mm. The theoretical (in vacuum) declared sensitivity is of the order of 1 ng/(cm2Hz). The fluidic apparatus consists of a GILSON peristaltic pump, two Tygon silicone tubes with diameters of 0.76 mm for the in and out flows, and a 30 μL cell containing the QR.After TBA-SH self-assembling, the interaction monitoring of humanα-thrombin (TB) (36700 Da) purchased from Haematologic Technologies Inc. was performed as described below. TB aliquots were solubilized in phosphate buffer 20 mM pH 7.4 in order to obtain 20, 50, and 100 nM solution concentrations. Each solution was injected into QCM flow chamber. A control experiment was performed monitoring the interaction between TBA and 5 μM Human Serum Albumin (HSA) purchased from Sigma-Aldrich.


### 2.4. Circular Dichroism

Circular Dichroism (CD) spectra were recorded at 25°C on a Jasco J-715 spectropolarimeter equipped with a Jasco JPT423S Peltier, using a 0.1 cm path length quartz cuvette, with a reaction volume of 500μL. The TBA-SH sample was prepared at 20 μM concentration in PBS buffer. The spectra were averaged over three scans. The buffer baseline was subtracted from each spectrum and the spectra were normalized to have zero at 320 nm. CD melting curve was registered as a function of temperature (range 20–90°C) for the quadruplex at its maximum Cotton effect wavelength.


### 2.5. Atomic Force Microscopy

20μL of TBA (2.7 mM in deionized water) and TB (2 μM in deionized water) was deposited on two pieces of crystalline silicon (Virginia Semiconductors, Inc.; p+ doped, 100 oriented, and 400 μm thick, with 0.003 Ωcm resistivity) and dried at room temperature. Samples were washed with deionized water and dried in a stream of nitrogen gas. Morphology of biomolecules deposited on silicon was investigated by Atomic Force Microscopy (AFM) using a XE-100 microscope from Park Systems. Surface imaging was obtained in noncontact mode using silicon/aluminium coated cantilevers (PPP-NCHR 10 M, Park Systems) 125 mm long with a resonance frequency between 200 and 400 kHz and a nominal force constant of 42 N/m. The scan frequency was typically 1 Hz per line. The scan area in surface analysis was 2 μm × 2 μm.


### 2.6. Spectroscopic Ellipsometry

Spectroscopic ellipsometry (SE) measurements were performed by a Jobin Yvon UVISEL-NIR phase modulated spectroscopic ellipsometer apparatus, at an incidence angle of 65° over the range 500–1600 nm with a resolution of 5 nm. The instrument measures the spectral variation of parametersIs and Ic related to the ellipsometric angles Ψ and Δ through the relation:(1)Is=sin⁡2Ψsin⁡ΔIc=sin⁡2Ψcos⁡Δ,where Ψ and Δ are defined from the fundamental equation of ellipsometry:(2)rprs=tan⁡ΨeiΔwith rp and rs being the complex reflection coefficients of the light polarized parallel and perpendicular to the incidence plane. Refractive indexes of TBA and TB and the thicknesses of biomaterials self-assembled on gold substrate were determined from the ellipsometric data analysis using Delta Psi software [27].


## 2.1. Aptamer Synthesis and Purification

The thiolated-thrombin binding aptamer (TBA-SH) (4726 Da) was synthesized by Expedite 8909 DNA synthesizer (Applied Biosystems, USA) using standard solid phaseβ-cyanoethylphosphoramidite chemistry on a Controlled Pore Glass (CPG) resin, as previously reported [26]. Last coupling step was performed with a proper thiol-modified phosphoramidite (dithiothreitol group, DTT). All the reagents and the CPG support were purchased from Glen Research. The oligomers were detached from the resin and deprotected by treatment with aqueous ammonia at 55°C, overnight. The combined filtrates and washing were purified through reverse phase HPLC (Purospher STAR, 5 μm, 250 × 10 mm, Merck), to allow the identification of our product as the most retained peak, by virtue of the presence of the trityl group protecting the thiol function. We used a linear gradient from 0% to 100% B for 30′ and flow rate 1.2 mL/min, where A is 0.1 M triethylammonium bicarbonate (TEAB) buffer, pH 7, and B is acetonitrile (ACN).After the purification of our product, the deprotection from the trityl group was performed. To this purpose, the lyophilized oligomer was dissolved in 0.1 M triethylammonium acetate (TEAA) buffer, to have a final concentration of 7 × 10−4M, and treated with 0.15 volumes of the final volume of 1 M aqueous silver nitrate solution. After 30 min of thoroughly mixing, 0.2 volumes of 1 M DTT solution were added. The solution was stirred for 5 min and then centrifuged to remove the silver DTT complex. The supernatant was collected and the precipitate was washed with TEAA 0.1 M and centrifuged for the second time. The combined supernatants were purified by Sep-Pak cartridges (C-18) to remove the excess of DTT. The obtained fractions were collected and dried under vacuum. Samples concentrations were determined in water by measuring the absorbance at 260 nm at 90°C using the nearest-neighbor calculated molar extinction coefficient of d[5′GGTTGGTGTGGTTGG3′] ε = 143,000 M−1cm−1.Finally, the sample was dissolved in PBS buffer (Sigma-Aldrich; 10 mM phosphate buffer, 2.7 mM KCl, 137 mM NaCl, pH 7.4), and the corresponding quadruplex was formed by heating the sample at 90°C and slowly cooling at room temperature.


## 2.2. Thiolated-TBA Self-Assembling

Self-assembling TBA-SH (4904.72 Da) on gold-coated quartz resonator was performed flowing 1μM TBA-SH solution in phosphate buffer 20 mM pH 7.4 for 100 min at room temperature. Three flowing cycles were performed in order to refresh the solution in contact with gold quartz surface.


## 2.3. Quartz Crystal Microbalance and Thrombin Monitoring

The mass variations were measured by a QCM instrument (Novaetech S.r.l., Italy), connected to a computer by a producer-released software. The quartz resonators (QRs) (IEV S.r.l., Italy) are characterized by a fundamental nominal frequency of 10 MHz, a blank diameter of 8 mm, and a gold lamina diameter of 4.5 mm. The theoretical (in vacuum) declared sensitivity is of the order of 1 ng/(cm2Hz). The fluidic apparatus consists of a GILSON peristaltic pump, two Tygon silicone tubes with diameters of 0.76 mm for the in and out flows, and a 30 μL cell containing the QR.After TBA-SH self-assembling, the interaction monitoring of humanα-thrombin (TB) (36700 Da) purchased from Haematologic Technologies Inc. was performed as described below. TB aliquots were solubilized in phosphate buffer 20 mM pH 7.4 in order to obtain 20, 50, and 100 nM solution concentrations. Each solution was injected into QCM flow chamber. A control experiment was performed monitoring the interaction between TBA and 5 μM Human Serum Albumin (HSA) purchased from Sigma-Aldrich.


## 2.4. Circular Dichroism

Circular Dichroism (CD) spectra were recorded at 25°C on a Jasco J-715 spectropolarimeter equipped with a Jasco JPT423S Peltier, using a 0.1 cm path length quartz cuvette, with a reaction volume of 500μL. The TBA-SH sample was prepared at 20 μM concentration in PBS buffer. The spectra were averaged over three scans. The buffer baseline was subtracted from each spectrum and the spectra were normalized to have zero at 320 nm. CD melting curve was registered as a function of temperature (range 20–90°C) for the quadruplex at its maximum Cotton effect wavelength.


## 2.5. Atomic Force Microscopy

20μL of TBA (2.7 mM in deionized water) and TB (2 μM in deionized water) was deposited on two pieces of crystalline silicon (Virginia Semiconductors, Inc.; p+ doped, 100 oriented, and 400 μm thick, with 0.003 Ωcm resistivity) and dried at room temperature. Samples were washed with deionized water and dried in a stream of nitrogen gas. Morphology of biomolecules deposited on silicon was investigated by Atomic Force Microscopy (AFM) using a XE-100 microscope from Park Systems. Surface imaging was obtained in noncontact mode using silicon/aluminium coated cantilevers (PPP-NCHR 10 M, Park Systems) 125 mm long with a resonance frequency between 200 and 400 kHz and a nominal force constant of 42 N/m. The scan frequency was typically 1 Hz per line. The scan area in surface analysis was 2 μm × 2 μm.


## 2.6. Spectroscopic Ellipsometry

Spectroscopic ellipsometry (SE) measurements were performed by a Jobin Yvon UVISEL-NIR phase modulated spectroscopic ellipsometer apparatus, at an incidence angle of 65° over the range 500–1600 nm with a resolution of 5 nm. The instrument measures the spectral variation of parametersIs and Ic related to the ellipsometric angles Ψ and Δ through the relation:(1)Is=sin⁡2Ψsin⁡ΔIc=sin⁡2Ψcos⁡Δ,where Ψ and Δ are defined from the fundamental equation of ellipsometry:(2)rprs=tan⁡ΨeiΔwith rp and rs being the complex reflection coefficients of the light polarized parallel and perpendicular to the incidence plane. Refractive indexes of TBA and TB and the thicknesses of biomaterials self-assembled on gold substrate were determined from the ellipsometric data analysis using Delta Psi software [27].


## 3. Results and Discussion

The synthetized sequence TBA-SH, namely, HS-(CH2)6-5′GGTTGGTGTGGTTGG-3′, was characterized by Circular Dichroism (CD) and CD melting in order to attest its ability to fold into monomolecular antiparallel chair-like G-quadruplex structure, adopted by the unmodified TBA in Na+- and K+-containing aqueous buffer. The results showed that TBA-SH folded into G-quadruplex structure in PBS buffer (the same used for all experiments on QCM sensor) despite the presence of thiol-modified alkyl chain at its 5′ terminus. The CD spectrum showed a positive maximum at 294 nm and a negative minimum at around 260 nm, which are indicative for a TBA-like antiparallel quadruplex arrangement [15, 28]. Moreover, the CD melting curve of TBA-SH recorded at 260 nm disclosed Tm of 33°C for the quadruplex to random coil transition, very close to Tm of the pure TBA in the same buffer (Figure 1) [16, 29].Figure 1
CD spectrum (a) and CD melting profile (b) of TBA-SH in PBS buffer.
(a)
(b)A preliminary characterization of TBA and TB molecule structures was performed by AFM measurements. To this aim, TBA and TB were independently adsorbed on flat silicon supports (average roughness < 2 nm), as described in Section2.5, and studied separately. The results of morphologic characterization at nanoscopic level are shown in Figure 2. In case of TBA (Figure 2(a)), large and irregular aggregates constituted by small features of few nanometers (5–10) could be detected. The biofilm thickness ranged between 20 and 140 nm. Quite differently, TB (Figure 2(b)) aggregated in a biolayer of average width of about 10 nm, but exposing some much larger structures, up to 800 nm, and with height included between 60 and 160 nm.Figure 2
AFM topography images and cross profile (first column) of TBA (a) and TB (b) deposited on silicon surfaces. Corresponding phase (second column) and 3D (third column) images.
(a)
(b)Real-time monitoring of TBA-SH self-assembling on gold and its interaction with TB was then quantified by QCM measurements, which is a well-established technique in quantification of TBA-TB interaction [30, 31]. In Figure 3, the oscillation frequency f of the quartz resonator (QR) used in our experiments is reported as function of time. The starting frequency, stabilized in phosphate buffer solutions, resulted in an absolute variation Δf of 160±10 Hz. According to Sauebrey’s equation and QCM/QR geometric features, reported in Section 2.3, the measured Δf corresponded to 0.112±0.007 μg of TBA-SH adsorbed onto QR. Three flowing cycles of TBA-SH solution were performed during self-assembling study, in order to refresh the solution in contact with QR: it can be noted that a stable self-assembling of TBA-SH on gold was achieved already after the first flowing cycle.Figure 3
Real-time monitoring of TBA-SH self-assembling onto QR surfaces.Extensive rinsing washes were performed to remove TBA-SH molecules not strongly bound to QR surface, and then real-time interaction monitoring with TB molecules was studied.Figure4 reports the frequency change Δf due to three binding cycles of TB solutions at increasing concentrations, compared to a control experiment performed by flowing Human Serum Albumin at 5 μM concentration. TB interaction caused Δf of 17, 5, and 9 Hz after injection of TB solutions with concentrations of 20, 50, and 100 nM, respectively. Each TB Δf corresponded to 11.9, 3.5, and 6.3 ng of TB adsorbed onto QR (Table 1), reaching a final TB adsorbed mass of 22 ng.Table 1
Comparison between TB mass injected and adsorbed onto QR surface.
TB (1st cycle)
TB (2nd cycle)
TB (3rd cycle)
Mass injected
22 ng
50 ng
110 ng
Δ
f
17 Hz
5 Hz
9 Hz
Mass adsorbed
11.9 ng
3.5 ng
6.3 ngFigure 4
Real-time monitoring of different concentrations (20, 50, and 100 nM) of TB by a TBA modified gold substrate (black line). Real-time monitoring of 5μM HSA by a TBA modified gold substrate (red line) as control.In order to exclude aspecific binding of TB on a bare gold quartz surface, 100 nM solution of TB was flowed in the microfluidic circuit right through the QR surface. Experimental data (see Figure S1 in Supplementary Material available online athttp://dx.doi.org/10.1155/2016/3561863) highlighted that TB injection produced Δf of only 3 Hz, which means that really few TB molecules stopped on QR surface, probably due to weak electrostatic aspecific interactions.TBA-TB strong binding was proved by stability of QR frequency values even after extensive rinsing steps succeeding TB injection, but this biomolecular interaction did not led to an irreversible complex: it is well known that TBA denatures at high temperature, thus releasing TB molecules [32, 33]. After TB binding experiment, the QR surface was rinsed at 55°C for 1 h and then used again in QCM set up in order to prove complete reversibility of QR signal (data reported in Supplementary Materials): on exposure to 20 nM and 50 nM injection of TB, Δf change of about 20 Hz resulted, confirming reversibility of the biosystem.The plot ofΔf as a function of TB concentration could be fitted by OriginLab Software™ (see Figure 5) Hill equation [34]:(3)y=ΔfmaxTBαKdα+TBα,where Δfmax is the saturation point; Kd is the affinity constant; [TB] is TB concentration; α is Hill coefficient [35, 36]. From the fit, we get an Hill coefficient equal to 1, which means that just one site of reaction was present, and the affinity of the biomolecule for its ligand was not dependent on whether or not other ligand molecules were already bound; therefore, the Hill equation was formally equivalent to a Langmuir equation [37]. Furthermore, we estimated a system affinity constant of 17.7±0.3 nM, a sensitivity of 0.42±0.03 Hz nM−1 (calculated in the range of linearity of system response), and a limit of detection (LOD) of 240±20 pM. Kd was found comparable to previous studies [38–42]. LOD value was found lower than other well-known very sensitive assays [43–45], but higher than QCM modified one [46].Figure 5
Quartz frequency variation versus TB concentration. Experimental data (black symbols) were fitted using Hill kinetic model.A parallel investigation of TBA-TB binding on gold surface was performed by using an all-optical, label-free characterization technique: the spectroscopic ellipsometry, generally useful for refractive indexes and biofilm thicknesses estimation [47–49]. The experimental data were fitted using the fixed index model for transparent media (data not shown here). Refractive indexes of 1.674 and 1.558 were obtained for TBA and for TB, respectively. Figure 6 shows Is and Ic experimental spectra in the case of thiolated-TBA self-assembled on gold substrate before and after the interaction with 110 nM thrombin, together with calculated spectra and material models used to fit them. TBA and TB were modeled by a simple homogeneous layer using the previously calculated refractive index. The fitting parameters are the film thicknesses. A TBA thickness of 4 (1) nm (χ2=3) was estimated analyzing the sample before the interaction with TB. After TB recognition, ellipsometry reveals that the presence of a second film of 13 (1) nm (χ2=5) was observed due to the presence of thrombin bound to TBA molecules. The results of this analysis were the average of three different measurements on the same sample, in order to take into account film inhomogeneities. A negative control performed on another sample of TBA assembled on gold-coated quartz after exposure to 5 μM HSA did not show any variation in biofilm thickness, demonstrating high selectivity of TBA-TB interaction.Figure 6
Measured and calculatedIs and Ic spectra of thiolated-TBA self-assembled on a gold substrate before (a) and after (b) interaction with TB.
(a)
(b)The surface concentration Γ (μg/cm2) of TBA biofilm self-assembled on gold substrate can be also calculated from ellipsometric data by using the Cuypers formula [50]:(4)Γ=0.1dMAnf2-1nf2+2,where A is the molar refractivity (cm3/mol), M is the molecular weight, and d and nf are the thickness and the refractive index of the biofilm (determined by SE).In Table2, we reported the parameters used to calculate Γ and the value of Γ, revealing a good surface coverage comparable to previous study [51]. The molar refractivity of TBA was obtained by adding the known values of its constituent atoms [50].Table 2
Surface concentration value of TBA.
Molecule
n
f
d (cm)
M (g/mol)
A (cm3/mol)
Γ (μg/cm2)
TBA-SH
1.674
(4 ± 1) × 10−7
4904.72
1022
0.07 ± 0.02


## 4. Conclusions

In this work, quartz crystal microbalance and spectroscopic ellipsometry are used to characterize the recognition of thrombin by thiolated-thrombin binding aptamer self-assembled on gold substrate. A selective and specific biomolecular interaction, characterized by low detection limit (240±20 pM) and good sensitivity (0.42±0.03 Hz nM−1), is demonstrated. The work provides a good starting point for the development of a nanostructured biosensor in order to realize a future Lab-on-Chip (LoC) device for vascular diseases early diagnosis.



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*Source: 3561863-2016-05-09.xml*