# Applicability of Isolates and Fractions of Plant Extracts in Murine Models in Type II Diabetes: A Systematic Review

Authors: Gabriela Diniz Pinto Coelho; Vanessa Soares Martins; Laura Vieira do Amaral; Rômulo Dias Novaes; Mariáurea Matias Sarandy; Reggiani Vilela Gonçalves
Journal: Evidence-Based Complementary and Alternative Medicine
 (2016)

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## Abstract

Type II diabetes mellitus is one of the most common public health problems worldwide. Its increasing prevalence in several countries and the difficult metabolic control of individuals with the disease justify studying strategies for primary prevention. The population has sought alternative and cheaper ways to treat the disease, including the use of plants considered medicinal by the population. In this study, we carried out a systematic review on the applicability of isolates and fractions of plant extracts in animal models in type II diabetes. A literature search was performed in MEDLINE/PubMed and Scopus databases. Studies using other experimental animals (horses, rabbits, and monkeys) and humans as well as articles in Chinese, German, and Russian were excluded. We assessed the quality of the studies included by using the criteria described in the ARRIVE guidelines. In general, the animals that received fractions or isolates presented reduced blood glucose levels, normalization of body weight and plasma insulin levels, and reduced total triglycerides and cholesterol. In addition, we observed wide variation among the analyzed parameters, which hindered comparison between the studies found. In further studies, standardized reports and experimental design would help to establish comparable study groups and advance the overall knowledge, thus facilitating translatability from animal data to human clinical conditions.


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## Body

## 1. Introduction

Diabetes mellitus (DM) is a syndrome caused by changes in the metabolism of carbohydrates, lipids, and proteins and may occur in two different forms: type I (10%) and type II (90%) [1]. Type I diabetes results from body inability to produce insulin [2], while type II may be caused by a failure in the production and secretion of insulin by the pancreas, due to insufficient production or a problem in the beta-cell receptors, thus decreasing the sensitivity of the target tissue to the metabolic effect of this hormone. This decreasing sensitivity is known as insulin resistance [3]. Patients with this disease present polydipsia (excessive thirst), polyuria (urine production over 2.5 liters/day), polyphagia (excessive avidity for food), and delayed wound healing [4]. Besides, poor glucose metabolism, reduced insulin signaling, excessive release of free fatty acids, and interleukin-6 are changes also considered important for analysis in clinical and preclinical studies [5].According to the World Health Organization (2012), diabetes mellitus accounts for 3.5% of the noncommunicable diseases in the world. The International Diabetes Federation (IDF) [6] estimates that, by 2013, more than 382 million people around the world had been affected by the disease, and the figures are increasing in all countries. Portugal, for example, is the second country in Europe with the highest prevalence of diabetes mellitus: 12.7 people with DM per 100 inhabitants in 2011 [7].In Brazil, according to estimates, there are more than 12 million people with type II diabetes mellitus (DM2) [8], mainly people who are over 40 years of age and obese. However, recent studies have shown a considerable increase in the number of children and teenagers with the disease, which may be associated with bad eating habits and lack of physical activity. This leads to increasing rates of obesity, which is considered a risk condition for the development of type II diabetes [9].The population has sought alternative and cheaper treatments for the disease, including the use of plants considered medicinal by the population. In several countries, such as Japan, China, and India, the use of medicinal plants and their derivatives is increasing, since they are considered simple, cheap, and effective treatment alternatives [10, 11]. There are some reports in the literature about the benefits of different herbal treatments on several metabolic changes caused by type II diabetes. The studies generally show positive effects of plant extracts on the normalization of body weight and decrease of glucose levels, total cholesterol, and triglycerides [12].Therefore, the use of phytotherapy has opened a new perspective for the management and treatment of metabolic diseases, such type II diabetes, since it is an affordable treatment [13]. However, the use of most of these plants has not been investigated [14, 15]. Research on plant extracts should be conducted to ensure that they are effective and safe for the population [16]. Due to these factors and the high number of type II diabetes carriers, the demand for less expensive therapies may significantly benefit population health [11].In vivo andin vitro studies are commonly used in biomonitoring research of plant extracts, aiming to identify their biological activity. The fractions are metabolites obtained by fractioning plant extracts, which provides a more specific analysis of the plant active principle [17]. These studies are important for the treatment of chronic diseases, including diabetes, since the treatment or control of these diseases is expensive and the number of affected people has increased considerably. Besides the fractions, the isolates obtained from plants have also played an important role in the treatment of metabolic disorders. After isolating a particular component of the crude extract, it is possible to ensure that the effects on the tissue are caused only by a specific constituent of the extract. This makes the molecule more attractive to the drug market, which aims to develop drugs from plants or other materials.A systematic review is based on predetermined criteria and consistent scientific evidence. It aims to collaborate with research selection and/or tools for the development of products based on original information [18], with well-defined criteria selection, to ensure the quality of the summarized studies and their reproducibility. Moreover, a conclusion providing new information based on filtered content is necessary [19]. Generally, due to its rigorous methods to identify, select, collect, and analyze data, this kind of study provides the highest level of scientific evidence. Therefore, our study aimed to make a descriptive and critical analysis of studies on the activity of plant fractions and isolates in the treatment of type II diabetes in animal models.


## 2. Material and Methods

### 2.1. Selection of Papers

The papers analyzed in this review were selected from two electronic databases, PubMed and Scopus, accessed on September 3, 2015, using the search filters: “animal model”, “plant extract”, and “diabetes mellitus type II”. These filters have been developed for the search on PubMed, according to the Medical Subject Headings (MeSH terms), used for a more efficient indexing of publications on the subject under study [20]. In order to expand the search, MeSH terms were combined with the title and abstract (TIAB). A standard filter was used [21] to identify all studies with animals in PubMed. The terms used to search on PubMed were adapted for the selection of Scopus publications, and the “animal model” filter was provided by the site itself (Supplemental Data 1 in Supplementary Material available online at http://dx.doi.org/10.1155/2016/3537163).The Prism guideline was used to develop this review [22]. After the papers were collected from the two electronic databases, the duplicates were excluded by comparing the title, author, year, and country. A screening was performed for title and abstract, guided by the eligibility criteria:in vivo experimental studies; studies using rats or diabetic mice; use of fractions or isolates of noncommercial plants; treatment of the main symptoms of type II diabetes; studies written in English or Portuguese.Next, all selected papers were obtained in full for a second screening, when all of them were examined to select those that met the criteria for the inclusion in the systematic review. Those unavailable on the internet were requested from their respective authors. When they did not respond, the studies were excluded. The entire search process, exclusion, and the number of selected papers were described in detail in the PRISMA Guideline (Figure1).Figure 1
Results obtained after the advanced search in the databases. After literature review, 39 papers were selected from Moher D, Liberati A, Tetzlaff J, Altman DG, and the PRISMA Group (2009). Preferred reporting items for systematic reviews and meta-analyses: the PRISMA Statement. PLoS Med 6(6): e1000097. doi: 10.1371/journal.pmed1000097. For more information, visitwww.prisma-statement.org. Prism: systematic review.


### 2.2. Qualitative Characteristics of Publications

After screening, the papers were reviewed. Table2 shows the description of the main characteristics of the studies. The following parameters were assessed: (1) publication features: author, year, and country; (2) experimental features: animal model, species, sample number, sex, weight, age, type of caging used, number of animals per cage, number of experimental groups and number of animals in each group, if randomization was made, and control groups; (3) treatment features: plant species used, name of the fraction or isolate, dose, route of administration, and treatment duration; (4) diabetes induction: drug used, dose, route of administration, and testing to prove diabetes occurrence (Table 1).Table 1
Table of qualitative descriptors of the studies of the effects of fractions and isolates from the plants on the treatment of the type II diabetes.
(a) Title Author/publication year Country Isolates [25] A Polysaccharide Extract of Mulberry Leaf Ameliorates Hepatic Glucose Metabolism and Insulin Signaling In Rats with Type 2 Diabetes Induced by High Fat-Diet and Streptozotocin Ren et al. (2015) China [26] Antidiabetic Activity of a Xanthone Compound, Mangiferin Miura et al. (2001) Japan [27] Anti-Diabetic Effect of a Novel N-Trisaccharide Isolated from Cucumis prophetarumon Streptozotocin-Nicotinamide Induced Type 2 Diabetic Rats Kavishankar and Lakshmidevi (2014) India [28] Antidiabetic Effects of the Cimicifuga racemosa Extract Ze 450 In Vitro and In Vivo in ob/ob Mice Moser et al. (2014) Switzerland [29] Borapetoside C from Tinospora crispa Improves Insulin Sensitivity in Diabetic Mice Ruan et al. (2012) Taiwan [30] Dehydrotrametenolic Acid Induces Preadipocyte Differentiation and Sensitizes Animal Models of Noninsulin-Dependent Diabetes Mellitus to Insulin Sato et al. (2002) Japan [31] Effects of Ingested Fruiting Bodies, Submerged Culture Biomass, and Acidic Polysaccharide Glucuronoxylomannan of Tremella mesenterica Retz.:Fr. on Glycemic Responses in Normal and Diabetic Rats Lo et al. (2006) Taiwan [32] Inhibition of Glycogen Synthase Kinase-3β by Falcarindiol Isolated from Japanese Parsley (Oenanthe javanica) Yoshida et al. (2013) Japan [33] Isolation and Antihyperglycemic Activity of Bakuchiol from Otholobiumpubescens (Fabaceae), a Peruvian Medicinal Pant Used for the Treatment of Diabetes Krenisky et al. (1999) USA [34] LBP-4a Improves Insulin Resistance via Translocation and Activation of GLUT4 in OLETF Rats Zhao et al. (2014) China [35] Meliacinolin: A Potent Α-Glucosidase and Α-Amylase Inhibitor Isolated from Azadirachta indica Leaves and In Vivo Antidiabetic Property in Streptozotocin-Nicotinamide-Induced Type 2 Diabetes in Mice Perez-Gutierrez and Damian-Guzman (2012) Mexico [36] Novel Terpenoid-Type Quinones Isolated from Pycnanthus angolensis of Potential Utility in the Treatment of Type 2 Diabetes Luo et al. (1999) USA [37] Platyconic Acid, A Saponin from Platycodi radix, Improves Glucose Homeostasis by Enhancing Insulin Sensitivity In Vitro and In Vivo Kwon et al. (2012) South Korea [38] Rhaponticin from rhubarb Rhizomes Alleviates Liver Steatosis and Improves Blood Glucose and Lipid Profiles in KK/Ay Diabetic Mice Chen et al. (2009) China [39] Rutin Potentiates Insulin Receptor Kinase to Enhance Insulin-Dependent Glucose Transporter 4 Translocation Hsu et al. (2014) Taiwan [40] Type 2 Antidiabetic Activity of Bergenin from the Roots of Caesalpinia digynaRottler Kumar et al. (2012) India  Fractions [41] A Study on Hypoglycaemic Health Care Function of Stigma maydis Polysaccharides Zhang et al. (2013) China [1] Antidiabetic Activities of Extract from Malva verticillataSeed via the Activation of AMP-Activated Protein Kinase Jeong and Song (2011) Korea [42] Antidiabetic Activity of Alkaloids of Aerva lanata Roots on Streptozotocin-Nicotinamide Induced Type-II Diabetes in Rats Agrawal et al. (2013) India [43] Antidiabetic Activity of Pterospermum acerifolium Flowers and Glucose Uptake Potential of Bioactive Fraction in L6 Muscle Cell Lines with Its HPLC Fingerprint Paramaguru et al. (2014) India [44] Antidiabetic Activity of Caesalpinia bonducella F. in Chronic Type 2 Diabetic Model in Long-Evans Rats and Evaluation of Insulin Secretagogue Property of Its Fractions on Isolated Islets Chakrabarti et al. (2005) India [45] Antidiabetic Effect of an Acidic Polysaccharide (TAP) from Tremella aurantiaand Its Degradation Product (TAP-H) Kiho et al. (2001) Japan [46] Antidiabetic Effect of Orally Administered Conophylline-Containing Plant Extract on Streptozotocin-Treated and Goto-Kakizaki Rats Fujii et al. (2009) Japan [47] Antidiabetic Effect of Total Flavonoids from Sanguisd raxonis  in Type 2 Diabetic Rats Chen et al. (2013) China [48] Antidiabetic Effects of Bitter Gourd Extracts in Insulin-Resistant db/db Mice Klomann et al. (2010) Germany [49] Anti-Diabetic Effects of Centratherum anthelminticum Seeds Methanolic Fraction on Pancreatic Cells, Β-TC6 and Its Alleviating Role in Type 2 Diabetic Rats Arya et al. (2012) Malaysia [17] Anti-Diabetic Effects of Polysaccharides from Talinum triangulare in Streptozotocin (STZ)-Induced Type 2 Diabetic Male Mice Xu et al. (2015) China [50] Anti-Diabetic Effects of the Acetone Fraction of Senna singueana Stem Bark in a Type 2 Diabetes Rat Model Ibrahim and Islam (2014) South Africa [51] Antidiabetic Potential of Polysaccharides from the White Oyster Culinary-Medicinal Mushroom Pleurotus florida (Higher Basidiomycetes) Ganeshpurkar et al. (2014) India [52] Antihyperglycemic Effects of Total Flavonoids from Polygonatum odoratum in STZ and Alloxan-Induced Diabetic Rats Shu et al. (2009) China [53] Antioxidant and Anti-Inflammatory Effects of a Hypoglycemic Fraction from Cucurbita ficifolia  Bouché in Streptozotocin-Induced Diabetic Mice Roman-Ramos et al. (2012) Mexico [54] Effect of Lycium barbarumPolysaccharide on the Improvement of Insulin Resistance in NIDDM Rats Zhao et al. (2005) China [55] Effects of Grape Seed Extract and Its Ethylacetate/Ethanol Fraction on Blood Glucose Levels in a Model of Type 2 Diabetes Hwang et al. (2009) South Korea [56] Hypoglycemic Effect of Astragaluspolysaccharide and Its Effect on PTP1B1 Wu et al. (2005) China [57] Hypoglycemic Effects of MDG-1, a Polysaccharide Derived from Ophiopogonjaponicus, in the ob/ob Mouse Model of Type 2 Diabetes Mellitus Xu et al. (2011) China [58] Isolation and Pharmacological Activities of the Tecoma stans Alkaloids Costantino et al. (2003) Italy [58] Potent Effects of the Total Saponins From Dioscorea nipponica Makino against Streptozotocin-Induced Type 2 Diabetes Mellitus in Rats Yu et al. (2015) China [59] Tocopherol from Seeds of Cucurbita pepo against Diabetes: Validation by In Vivo Experiments Supported by Computational Docking Bharti et al. (2013) India [60] Trapa natans L. Root Extract Suppresses Hyperglycemic and Hepatotoxic Effects in STZ-Induced Diabetic Rat Model Kharbanda et al. (2014) India  (b) Title Animal model/strain Number of animals Sex Age Weight Housing of animals Animals per cage Groups and number of animals in each group Randomization Isolates [25] A Polysaccharide Extract of Mulberry Leaf Ameliorates Hepatic Glucose Metabolism and Insulin Signaling in Rats with Type 2 Diabetes Induced by High Fat-Diet and Streptozotocin Wistar rats 18 M 7 w 200 g Cages 5 3 groups (6 in each group) Yes [26] Antidiabetic Activity of a Xanthone Compound, Mangiferin Mice ? M 12 w ? ? ? ? ? [27] Anti-Diabetic Effect of a Novel N-Trisaccharide Isolated from Cucumis prophetarumon Streptozotocin-Nicotinamide Induced Type 2 Diabetic Rats Wistar rats 36 M&F ? 150–180 g Cages ? 6 groups (6 animals in each) ? [28] Antidiabetic Effects of the Cimicifuga racemosa Extract Ze 450 In Vitro and In Vivo in ob/ob Mice ob/ob mice 68 M 7-8 w ? Cages 1 8 groups (8 animals in each) ? [29] Borapetoside C from Tinospora crispa Improves Insulin Sensitivity in Diabetic Mice Mice ? M 8–10 w ? ? ? ? ? [30] Dehydrotrametenolic Acid Induces Preadipocyte Differentiation and Sensitizes Animal Models of Noninsulin-Dependent Diabetes Mellitus to Insulin C57BLK mice ? M 7 w ? ? ? ? ? [31] Effects of Ingested Fruiting Bodies, Submerged Culture Biomass, and Acidic Polysaccharide Glucuronoxylomannan of Tremella mesenterica Retz.:Fr. on Glycemic Responses in Normal and Diabetic Rats Wistar rats 102 M ? 200 g Cages 1 6 groups (12 animals in each group) and 3 groups (10 animals in each group) Yes [32] Inhibition of Glycogen Synthase Kinase-3β by Falcarindiol Isolated from Japanese Parsley (Oenanthe javanica) GK rats 12 M ? ? Cages 1 2 groups (6 animals in each group) ? [33] Isolation and Antihyperglycemic Activity of Bakuchiol from Otholobiumpubescens (Fabaceae), a Peruvian Medicinal Plant Used for the Treatment of Diabetes C57BL/K mice & Sprague- Dawley rats ? M 8 w ? ? 4 ? Groups (8 animals in each group) ? [34] LBP-4a Improves Insulin Resistance via Translocation and Activation of GLUT4 in OLETF Rats Otsuka Long-Evans Tokushima Fatty (OLETF) rats 18 M 26 w ? ? ? 3 groups (6 animals in each group) Yes [35] Meliacinolin: A Potent Α-Glucosidase and Α-Amylase Inhibitor Isolated from Azadirachta indica Leaves and In Vivo Antidiabetic Property in Streptozotocin-Nicotinamide-Induced Type 2 Diabetes in Mice ?/mice 42 M ? 20–25 g Cages 2 7 groups (6 animals in each group) ? [36] Novel Terpenoid-Type Quinones Isolated from Pycnanthus angolensis of Potential Utility in the Treatment of Type 2 Diabetes C57BLK mice ? M 7-8 w ? Cages 4 ? groups (5–8 animals in each group) ? [37] Platyconic Acid, a Saponin from Platycodi radix, Improves Glucose Homeostasis by Enhancing Insulin Sensitivity In Vitro and In Vivo C57BLK mice 90 M 8–10 w ? ? 1 6 groups (15 animals in each group) Yes [38] Rhaponticin from rhubarb Rhizomes Alleviates Liver Steatosis and Improves Blood Glucose and Lipid Profiles in KK/Ay Diabetic Mice Mice 30 M&F 8–12 w ? Cages 1 3 groups (10 animals in each group) Yes [39] Rutin Potentiates Insulin Receptor Kinase to Enhance Insulin-Dependent Glucose Transporter 4 Translocation Mice ? M 8 w ? ? ? ? ? [40] Type 2 Antidiabetic Activity of Bergenin from the Roots of  Caesalpinia digyna Rottler Charles Foster Albino rats 30 M ? ? ? ? 5 groups (6 animals in each group) ?  Fractions [41] A Study on Hypoglycaemic Health Care Function of Stigma maydis Polysaccharides SPF km mice ? M ? 20 ± 2 g ? ? 7 groups (animals in each group?) Yes [1] Antidiabetic Activities of Extract from Malva verticillataSeed via the Activation of AMP-Activated Protein Kinase. C57BLK mice ? ? 5 w ? Cages 5 5 groups (animals in each group?) ? [42] Antidiabetic Activity of Alkaloids of Aerva lanata Roots on Streptozotocin-Nicotinamide Induced Type-II Diabetes in Rats Wistar rats ? M ? 200–250 g ? ? ? ? [43] Antidiabetic Activity of Pterospermum acerifoliumFlowers and Glucose Uptake Potential of Bioactive Fraction in L6 Muscle Cell Lines with Its HPLC Fingerprint Albino Wistar rats 66 M ? 180–200 g Cages ? 11 groups (6 animals in each group) ? [44] Antidiabetic Activity of Caesalpinia bonducella F. in Chronic Type 2 Diabetic Model in Long-Evans Rats and Evaluation of Insulin Secretagogue Property of Its Fractions on Isolated Islets Long-Evans rats ? M&F 12–14 w 150 g ? ? 5 groups (6–9 animals in each group) ? [45] Antidiabetic Effect of an Acidic Polysaccharide (TAP) from Tremella aurantiaand Its Degradation Product (TAP-H) KK- Ay-TA mice ? M 5 w 25–30 g ? 1 3 groups (animals in each group?) ? [46] Antidiabetic Effect of Orally Administered Conophylline-Containing Plant Extract on Streptozotocin-Treated and Goto-Kakizaki Rats Goto-Kakizaki rats 14 M 5 w ? ? ? 3 groups (animals in each group?) Yes [47] Antidiabetic Effect of Total Flavonoids from Sanguis draxonis  in Type 2 Diabetic Rats Sprague-Dawley rats 72 M ? 180–220 g ? ? 6 groups (6 animals in each group) for SD; 6 groups (6 animals in each group) for SDF ? [48] Antidiabetic Effects of Bitter Gourd Extracts in Insulin-Resistant db/db Mice db/db mice 45 M 5 w ? Cages 1 5 groups (9 animals in each group) ? [49] Anti-Diabetic Effects of Centratherum anthelminticum Seeds Methanolic Fraction on Pancreatic Cells, Β-TC6 and Its Alleviating Role in Type 2 Diabetic Rats Sprague-Dawley rats 72 M&F ? 180–200 g ? ? 12 groups (6 animals in each group) ? [17] Anti-Diabetic Effects of Polysaccharides from Talinum triangulare in Streptozotocin (STZ)-Induced Type 2 Diabetic Male Mice SPF km mice 50 M ? 20 ± 2 g ? ? 5 groups (10 animals in each group) Yes [50] Anti-Diabetic Effects of the Acetone Fraction of Senna singueana Stem Bark in a Type 2 Diabetes Rat Model Sprague-Dawley rats 48 M 6 w 207.60 ± 4.27 g Cage 2 6 groups (8 animals in each group) Yes [51] Antidiabetic Potential of Polysaccharides from the White Oyster Culinary-Medicinal Mushroom Pleurotus florida (Higher Basidiomycetes) Wistar rats 20 M ? 150–200 g ? ? 4 groups (5 animals in each group) ? [52] Antihyperglycemic Effects of Total Flavonoids from Polygonatum odoratum in STZ and Alloxan-Induced Diabetic Rats Sprague-Dawley rats ? ? ? 220 ± 4.5 g Special animal house ? ? ? [53] Antioxidant and Anti-Inflammatory Effects of a Hypoglycemic Fraction from Cucurbita ficifolia  Bouché in Streptozotocin-Induced Diabetic Mice CD-1 mice ? M ? 30–35 g ? ? ? ? [54] Effect of Lycium barbarumPolysaccharide on the Improvement of Insulin Resistance in NIDDM Rats Wistar rats 40 M ? 230–250 g Cages ? ? Yes [55] Effects of Grape Seed Extract and Its Ethylacetate/Ethanol Fraction on Blood Glucose Levels in a Model of Type 2 Diabetes C57BL/KsJ-leprdb/leprdb mice 98 M 3 w 9.7–14.2 g Conventional state ? 7 groups (14 in each group) ? [56] Hypoglycemic Effect of Astragaluspolysaccharide and Its Effect on PTP1B1 Sprague-Dawley rat 34 M 8 w 200 g ? 5 4 groups (2 groups with 10 animals and 2 groups with 12 animals in each) ? [57] Hypoglycemic Effects of MDG-1, a Polysaccharide Derived from Ophiopogonj aponicus, in the ob/ob Mouse Model of Type 2 Diabetes Mellitus ob/ob mice ?  6-7 w ? ? ? 4 groups (8 animals in each group) ? [58] Isolation and Pharmacological Activities of the Tecoma stans Alkaloids C57BL/KsJ db/db mice ? M 8 w ? ? ? ? ? [61] Potent Effects of the Total Saponins from Dioscorea nipponica Makino against Streptozotocin-Induced Type 2 Diabetes Mellitus in Rats Wistar rats 70 M ? 190–200 g Cages 1 7 groups (8 animals in each group) Yes [59] Tocopherol from Seeds of Cucurbita pepo against Diabetes: Validation by In Vivo Experiments Supported by Computational Docking Albino Wistar rats 24 M ? 150–160 g ? ? 4 groups (6 animals in each group) ? [60] Trapa natans L. Root Extract Suppresses Hyperglycemic and Hepatotoxic Effects in STZ-Induced Diabetic Rat Model Albino Wistar rats 90 ? ? 150–200 g Cages ? 15 groups (6 animals in each group) ?  (c) Title Plant species Isolate/fraction Administration Doses used Duration of treatment Isolates [25] A Polysaccharide Extract of Mulberry Leaf Ameliorates Hepatic Glucose Metabolism and Insulin Signaling in Rats with Type 2 Diabetes Induced by High Fat-Diet and Streptozotocin Mulberry Mulberry leaf polysaccharide Gavage 200 mg/kg 6 days of treatment [26] Antidiabetic Activity of a Xanthone Compound, Mangiferin Anemarrhena asphodeloidesBunge Mangiferin Orally 30 mg/kg ? [27] Anti-Diabetic Effect of a Novel N-Trisaccharide Isolated from Cucumis prophetarum on Streptozotocin-Nicotinamide Induced Type 2 Diabetic Rats Cucumis prophetarum N-Trisaccharide Gavage 50, 5 mg/kg 28 days of treatment [28] Antidiabetic Effects of the Cimicifuga racemosa Extract Ze 450 In Vitro and In Vivo in ob/ob Mice Cimicifuga racemosa Ze 450 Gavage 10, 30, 90 mg/kg 7 days of treatment [29] Borapetoside C from Tinospora crispa Improves Insulin Sensitivity in Diabetic Mice Tinospora crispa Borapetoside C Orally 5 mg/kg 4 weeks of treatment [30] Dehydrotrametenolic Acid Induces Preadipocyte Differentiation and Sensitizes Animal Models of Noninsulin-Dependent Diabetes Mellitus to Insulin Poria cocosWolf Dehydrotrametenolic acid Gavage 110 mg/kg 14 days of treatment [31] Effects of Ingested Fruiting Bodies, Submerged Culture Biomass, and Acidic Polysaccharide Glucuronoxylomannan of Tremella mesenterica Retz.:Fr. on Glycemic Responses in Normal and Diabetic Rats Tremella mesenterica Acidic polysaccharide glucuronoxylomannan (GXM) Gavage 1 g/kg 15 days of treatment [32] Inhibition of Glycogen Synthase Kinase-3β by Falcarindiol Isolated from Japanese Parsley (Oenanthe javanica) Oenanthe javanica falcarindiol Orally 15 mg/kg ? [33] Isolation and Antihyperglycemic Activity of Bakuchiol from Otholobiumpubescens (Fabaceae), a Peruvian Medicinal Plant Used for the Treatment of Diabetes Otholobium pubescens Bakuchiol Gavage 1, 150, 250 mg/kg 2 weeks of treatment [34] LBP-4a Improves Insulin Resistance via Translocation and Activation of GLUT4 in OLETF Rats Lycium barbarum Lycium barbarum polysaccharide (LBP-4a) Orally 10 mg/kg 4 weeks of treatment [35] Meliacinolin: A Potent Α-Glucosidase and Α-Amylase Inhibitor Isolated from Azadirachta indica Leaves and In Vivo Antidiabetic Property in Streptozotocin-Nicotinamide-Induced Type 2 Diabetes in Mice Azadirachta indica Meliacinolin Orally by gastric intubations 20 mg/kg 28 days of treatment [36] Novel Terpenoid-Type Quinones Isolated from Pycnanthus angolensis of Potential Utility in the Treatment of Type 2 Diabetes Pycnanthus angolensis Novel terpenoid-type quinones (SP-18904 and SP-18905) Gavage 100 mg/kg 4 days of treatment [37] Platyconic Acid, a Saponin from Platycodi radix, Improves Glucose Homeostasis by Enhancing Insulin Sensitivity In Vitro and In Vivo Platycodi radix Platyconic acid (PA), platycodin D (PD), platycoside E (PE), and saponin with low activity (DPE) Gavage 20 mg/kg 8 weeks of treatment [38] Rhaponticin from rhubarb Rhizomes Alleviates Liver Steatosis and Improves Blood Glucose and Lipid Profiles in KK/Ay Diabetic Mice Rheum franzenbachiiMunt Rhaponticin Orally 125 mg/kg 4 weeks of treatment [39] Rutin Potentiates Insulin Receptor Kinase to Enhance Insulin-Dependent Glucose Transporter 4 Translocation Toona sinensis Flavonoid rutin Gavage 25 mg/kg ? [40] Type 2 Antidiabetic Activity of Bergenin from the Roots of Caesalpinia digyna Rottler Caesalpinia digynaRottler Bergenin Orally 2.5, 5, and 10 mg/kg 14 days of treatment  Fractions [41] A Study on Hypoglycaemic Health Care Function of Stigma maydis Polysaccharides Stigma maydis Stigma maydis polysaccharides ? 20 mg/kg 4 weeks of treatment [1] Antidiabetic Activities of Extract from Malva verticillata Seed via the Activation of AMP-Activated Protein Kinase Malva verticillata Ethanol extract ofM. verticillata and N-hexane (MVE-H) Orally 3 different concentrations of MVE-H (10, 20, or 40 mg/kg) 4 weeks of treatment [42] Antidiabetic Activity of Alkaloids of Aerva lanata Roots on Streptozotocin-Nicotinamide Induced Type-II Diabetes in Rats Aerva lanataLinn. The partially purified alkaloid basified toluene fraction (PPABTF) Orally 10, 20 mg/kg 2 weeks of treatment [43] Antidiabetic Activity of Pterospermum acerifolium Flowers and Glucose Uptake Potential of Bioactive Fraction in L6 Muscle Cell Lines with Its HPLC Fingerprint Pterospermum acerifolium Ethyl acetate fraction (PAFEF) and subfractions PAFE1, PAFE2, and e PAFE3 Intragastric tube 200, 400 mg/kg, and 15, 30 mg/kg 30 days of treatment [44] Antidiabetic Activity of Caesalpinia bonducella F. in Chronic Type 2 Diabetic Model in Long-Evans Rats and Evaluation of Insulin Secretagogue Property of Its Fractions on Isolated Islets Caesalpinia bonducellaF. Caesalpinia bonducellaaqueous and alcoholic extracts (BM-170 and BM-171) Orally 250 mg/kg 28 days of treatment [45] Antidiabetic Effect of an Acidic Polysaccharide (TAP) from Tremella aurantiaand Its Degradation Product (TAP-H) Tremella aurantia Acidic polysaccharide (TAP) and the degradation product (TAP-H) Orally 0.5 g/L, 1.5 g/L 10 weeks of treatment [46] Antidiabetic Effect of Orally Administered Conophylline-Containing Plant Extract on Streptozotocin-Treated and Goto-Kakizaki Rats Tabernaemontana divaricata The Crude Conophylline Preparation I (CCP-I) Orally 200, 50 g/kg 15 days of treatment [47] Antidiabetic Effect of Total Flavonoids from Sanguis draxonis  in Type 2 Diabetic Rats Dracaena cochinchinensis Sanguis  draxonis (SD) and total flavonoids from SD (SDF) Gavage ? 21 days of treatment [48] Antidiabetic Effects of Bitter Gourd Extracts in Insulin-Resistant db/db Mice Momordica charantia The lipid fraction, the saponin fraction, or the hydrophilic residue of bitter gourd Orally 150 mg/kg 5 weeks of treatment [49] Anti-Diabetic Effects of Centratherum anthelminticum Seeds Methanolic Fraction on Pancreatic Cells, Β-TC6 and Its Alleviating Role in Type 2 Diabetic Rats Centratherum anthelminticum Crude methanolic fraction (CAMF) Injected intraperitoneally (IP) or orally 50 and 100 mg/kg 4 weeks of treatment [17] Anti-Diabetic Effects of Polysaccharides from Talinum triangulare in Streptozotocin (STZ)-Induced Type 2 Diabetic Male Mice Talinum triangulare Polysaccharides obtained fromTalinum triangulare (TTP) Orally 150 and 300 mg/kg 2 weeks of treatment [50] Anti-Diabetic Effects of the Acetone Fraction of Senna singueana Stem Bark in a Type 2 Diabetes Rat Model Senna singueana Senna singueana acetone fraction (SSAF) Orally 150 mg/kg and 300 mg/kg 4 weeks of treatment [51] Antidiabetic Potential of Polysaccharides from the White Oyster Culinary-Medicinal Mushroom Pleurotus florida (Higher Basidiomycetes) Pleurotus florida P. florida polysaccharides (PFPs) Orally 200 and 400 mg/kg 21 days of treatment [52] Antihyperglycemic Effects of Total Flavonoids from Polygonatum odoratum in STZ and Alloxan-Induced Diabetic Rats Polygonatum odoratum Total flavonoids ofPolygonatum (P) odoratum (TFP) Orally 50, 100, and 200 mg/kg 30 days of treatment [53] Antioxidant and Anti-Inflammatory Effects of a Hypoglycemic Fraction from Cucurbita ficifolia  Bouché in Streptozotocin-Induced Diabetic Mice Cucurbita ficifolia Aqueous-precipitate fraction (AP-fraction) Gavage 200 mg/kg 15 days of treatment [54] Effect of Lycium barbarumPolysaccharide on the Improvement of Insulin Resistance in NIDDM Rats Lycium barbarum Lycium barbarum polysaccharide (LBP) Orally 10 mg/kg 3 weeks of treatment [55] Effects of Grape Seed Extract and Its Ethylacetate/Ethanol Fraction on Blood Glucose Levels in a Model of Type 2 Diabetes Vitis viniferagrape Grape seed extract (GSE), ethylacetate (e), and ethylacetate/ethanol (ee) Orally 50 and 30 mg/kg 8 weeks of treatment [56] Hypoglycemic Effect of Astragaluspolysaccharide and Its Effect on PTP1B1 Astragalus membranaceus Astragalus polysaccharide (APS) Orally 400 mg/kg 5 weeks of treatment [57] Hypoglycemic Effects of MDG-1, a Polysaccharide Derived from Ophiopogon japonicus, in the ob/ob Mouse Model of Type 2 Diabetes Mellitus Ophiopogon japonicus Water-solubleβ-d-fructan (MDG-1) Intragastrically 150 and 300 mg/kg 23 days of treatment [58] Isolation and Pharmacological Activities of the Tecoma stans Alkaloids Tecoma stans Tecoma stans alkaloids Gavage 50 mg/kg and 63.4 mg/kg 7 days of treatment [61] Potent Effects of the Total Saponins from Dioscorea nipponica Makino against Streptozotocin-Induced Type 2 Diabetes Mellitus in Rats Dioscorea nipponica Saponins fromD. nipponicaMakino (TSDN) Orally 200, 100, and 50 mg/kg 12 weeks of treatment [59] Tocopherol from Seeds of Cucurbita pepo against Diabetes: Validation by In Vivo Experiments Supported by Computational Docking Cucurbita pepo Tocopherol ? 2, 5 g/kg 6 weeks of treatment [60] Trapa natans L. Root Extract Suppresses Hyperglycemic and Hepatotoxic Effects in STZ-Induced Diabetic Rat Model Trapa natansL. Methanol fraction, chloroform fraction, and petroleum ether fraction Orally 50, 100, and 200 mg/kg 15 days of treatment  (d) Title Drug for diabetes induction Route of induction Hyperglycemia Control of glycemia Insulin tolerance test Isolates [25] A Polysaccharide Extract of Mulberry Leaf Ameliorates Hepatic Glucose Metabolism and Insulin Signaling in Rats with Type 2 Diabetes Induced by High Fat-Diet and Streptozotocin Diet with 41.2% fat and a low-dose STZ (35 mg/kg body weight) Intraperitoneal Glucose ≥ 7.8 mmol/L ? Yes [26] Antidiabetic Activity of a Xanthone Compound, Mangiferin Genetically modified — — ? Yes [27] Anti-Diabetic Effect of a Novel N-Trisaccharide Isolated from Cucumis prophetarum on Streptozotocin-Nicotinamide Induced Type 2 Diabetic Rats Nicotinamide (NA) at 230 mg/kg and STZ at 65 mg/kg Intraperitoneal Glucose ≥ 250 mg/dL Yes Yes [28] Antidiabetic Effects of the Cimicifuga racemosa Extract Ze 450 In Vitro and In Vivo in ob/ob Mice Genetically modified — — ? ? [29] Borapetoside C from Tinospora crispa Improves Insulin Sensitivity in Diabetic Mice STZ 150 mg/kg Intraperitoneal Glucose ≥ 150 mg/dL Yes Yes [30] Dehydrotrametenolic Acid Induces Preadipocyte Differentiation and Sensitizes Animal Models of Noninsulin-Dependent Diabetes Mellitus to Insulin Genetically modified — — ? ? [31] Effects of Ingested Fruiting Bodies, Submerged Culture Biomass, and Acidic Polysaccharide Glucuronoxylomannan of Tremella mesenterica Retz.:Fr. on Glycemic Responses in Normal and Diabetic Rats STZ (65 mg/kg) and nicotinamide (200 mg/kg) Intraperitoneal Glucose > 250 mg/100 mL Yes Yes [32] Inhibition of Glycogen Synthase Kinase-3β by Falcarindiol Isolated from Japanese Parsley (Oenanthe javanica) Genetically modified — — ? Yes [33] Isolation and Antihyperglycemic Activity of Bakuchiol from Otholobiumpubescens (Fabaceae), a Peruvian Medicinal Plant Used for the Treatment of Diabetes STZ 50 mg/kg Intravenous Glucose 300–600 mg/dL Yes ? [34] LBP-4a Improves Insulin Resistance via Translocation and Activation of GLUT4 in OLETF Rats Genetically modified — — ? Yes [35] Meliacinolin: A Potent Α-Glucosidase and Α-Amylase Inhibitor Isolated from Azadirachta indica Leaves and In Vivo Antidiabetic Property in Streptozotocin-Nicotinamide-Induced Type 2 Diabetes in Mice Nicotinamide (120 mg/kg) and STZ (60 mg/kg) Intraperitoneal Glucose > 250 mg/dL Yes ? [36] Novel Terpenoid-Type Quinones Isolated from Pycnanthus angolensis of Potential Utility in the Treatment of Type 2 Diabetes STZ 150 mg/kg Intravenous Glucose 300–600 mg/dL Yes Yes [37] Platyconic Acid, a Saponin from Platycodi radix, Improves Glucose Homeostasis by Enhancing Insulin Sensitivity In Vitro and In Vivo STZ 20 mg/kg ? ? Yes Yes [38] Rhaponticin from rhubarb Rhizomes Alleviates Liver Steatosis and Improves Blood Glucose and Lipid Profiles in KK/Ay Diabetic Mice STZ/? ? ? Yes ? [39] Rutin Potentiates Insulin Receptor Kinase to Enhance Insulin-Dependent Glucose Transporter 4 Translocation Insulin receptor antagonist S960 (50 nmol/kg) Intravenous injection ? Yes ? [40] Type 2 Antidiabetic Activity of Bergenin from the Roots of Caesalpinia digyna Rottler STZ (65 mg/kg) and nicotinamide (110 mg/kg) Intraperitoneal injection Glucose 200 mg/dL Yes Yes  Fractions [41] A Study on Hypoglycaemic Health Care Function of Stigma maydis Polysaccharides High fat-diet and low-dose alloxan (90 mg/kg) Intraperitoneal Glucose ≥ 10 mmol/L Yes ? [1] Antidiabetic Activities of Extract from Malva verticillata Seed via the Activation of AMP-Activated Protein Kinase Genetically modified — — Yes ? [42] Antidiabetic Activity of Alkaloids of Aerva lanata Roots on Streptozotocin-Nicotinamide Induced Type-II Diabetes in Rats STZ and nicotinamide Intraperitoneal ? Yes ? [43] Antidiabetic Activity of Pterospermum acerifolium Flowers and Glucose Uptake Potential of Bioactive Fraction in L6 Muscle Cell Lines with Its HPLC Fingerprint STZ (60 mg/kg) and nicotinamide (120 mg/kg) Intraperitoneal Glucose > 250 mg/dL Yes ? [44] Antidiabetic Activity of Caesalpinia bonducella F. in Chronic Type 2 Diabetic Model in Long-Evans Rats and Evaluation of Insulin Secretagogue Property of Its Fractions on Isolated Islets STZ 90 mg/kg Intraperitoneal injection Glucose 7–12 mmol/L Yes Yes [45] Antidiabetic Effect of an Acidic Polysaccharide (TAP) from Tremella aurantiaand Its Degradation Product (TAP-H) Genetically modified — — ? Yes [46] Antidiabetic Effect of Orally Administered Conophylline-Containing Plant Extract on Streptozotocin-Treated and Goto-Kakizaki Rats Streptozotocin 60 mg/kg Intraperitoneal Glucose > 250 mg/dL Yes ? [47] Antidiabetic Effect of Total Flavonoids from Sanguis draxonis  in Type 2 Diabetic Rats High fat-diet and a singular injection of streptozotocin (STZ) (35 mg/kg) Intraperitoneal Glucose ≥ 11.1 mmol/L Yes Yes [48] Antidiabetic Effects of Bitter Gourd Extracts in Insulin-Resistant db/db Mice Genetically modified — — ? ? [49] Anti-Diabetic Effects of Centratherum anthelminticum Seeds Methanolic Fraction on Pancreatic Cells, Β-TC6 and Its Alleviating Role in Type 2 Diabetic Rats Streptozotocin (STZ) (65 mg/kg) Intraperitoneal Glucose 22–26 mmol/L Yes ? [17] Anti-Diabetic Effects of Polysaccharides from Talinum triangulare in Streptozotocin (STZ)-Induced Type 2 Diabetic Male Mice Streptozotocin (STZ) (70 mg/kg) Intraperitoneal ? Yes ? [50] Anti-Diabetic Effects of the Acetone Fraction of Senna singueana Stem Bark in a Type 2 Diabetes Rat Model STZ 40 mg/kg Intraperitoneal injection Glucose > 18 mmol/L Yes ? [51] Antidiabetic Potential of Polysaccharides from the White Oyster Culinary-Medicinal Mushroom Pleurotus florida (Higher Basidiomycetes) STZ 50 mg/kg Intraperitoneal Glucose > 200 mg/100 mL Yes ? [52] Antihyperglycemic Effects of Total Flavonoids from Polygonatum odoratum in STZ and Alloxan-Induced Diabetic Rats Alloxan (100 and 120 mg/kg) Intraperitoneal Glucose > 11.0 mmol/L Yes ? [53] Antioxidant and Anti-Inflammatory Effects of a Hypoglycemic Fraction from Cucurbita ficifoliaBouché in Streptozotocin-Induced Diabetic Mice STZ 137 mg/kg Intraperitoneal ? ? ? [54] Effect of Lycium barbarumPolysaccharide on the Improvement of Insulin Resistance in NIDDM Rats STZ 50 mg/kg Intraperitoneal Glucose > 16 mmol/L Yes Yes [55] Effects of Grape Seed Extract and Its Ethylacetate/Ethanol Fraction on Blood Glucose Levels in a Model of Type 2 Diabetes Genetically modified — — Yes ? [56] Hypoglycemic Effect of Astragalus Polysaccharide and Its Effect on PTP1B1 STZ 30 mg/kg Intravenous injection Glucose > 6.7 mmol/L Yes Yes [57] Hypoglycemic Effects of MDG-1, a Polysaccharide Derived from Ophiopogonjaponicus, in the ob/ob Mouse Model of Type 2 Diabetes Mellitus Genetically diabetic model — — Yes Yes [58] Isolation and Pharmacological Activities of the Tecoma stans Alkaloids Genetically diabetic model — — ? Yes [61] Potent Effects of the Total Saponins from Dioscorea nipponica Makino against Streptozotocin-Induced Type 2 Diabetes Mellitus in Rats STZ 30 mg/kg Intraperitoneal Glucose > 16.7 mmol/L Yes Yes [59] Tocopherol from Seeds of Cucurbita pepo against Diabetes: Validation by In Vivo Experiments Supported by Computational Docking PX-407 solution (10 mg/kg) ? Blood glucose level of 200 mg/dL or higher Yes Yes [60] Trapa natans L. Root Extract Suppresses Hyperglycemic and Hepatotoxic Effects in STZ-Induced Diabetic Rat Model STZ (45 mg/kg) Intraperitoneal Glucose > 200 mg/dL Yes ?Table 2
Biases analyses (ARRIVE) of the studies of the effects of fractions and isolates from the plants in the treatment of the type II diabetes.
Isolates Fractions References Miura, et al., 2001 [26] Kavishankar and Lakshmidevi, 2014 [27] Moser et al., 2014 [28] Chen et al., 2009 [38] Ruan et al., 2012 [29] Sato et al., 2002 [30] Lo et al., 2006 [31] Yoshida et al., 2013 [32] Zhao et al., 2014 [34] Kumar et al., 2012 [40] Luo et al., 1999 [36] Perez-Gutierrez and Damian-Guzman, 2012 [35] Kwon et al., 2012 [37] Krenisky et al., 1999 [33] Hsu  et al., 2014 [39] Ren et al., 2015 [25] Agrawal et al., 2013 [42] Zhang et al., 2013 [41] Jeong and Song, 2011 [1] Chakrabarti et al., 2005 [44] Paramaguru et al., 2014 [43] Kiho et al., 2001 [45] Fujii et al., 2009 [46] Chen et al., 2013 [47] Arya et al., 2012 [49] Klomann et al., 2010 [48] Arya et al., 2012 [49] Xu et al., 2015 [17] Ibrahim and Islam, 2014 [50] Ganeshpurkar et al., 2014 [51] Shu et al., 2009 [52] Zhao et al., 2005 [54] Hwang et al., 2009 [55] Wu et al., 2005 [56] Xu et al., 2011 [57] Costantino et al., 2003 [58] Yu et al., 2015 [61] Kharbanda et al., 2014 [60] Roman-Ramos et al., 2012 [53] Accurate and concise description of the content of the article  X X X X X X X X X X  X X  X X   X X  X X X X X X X X X X X X X X X X X 33 84.62%  Abstract Summary of the background, research objectives, methods, main findings, and conclusions X X X X X  X X X X X X X X X X X X X   X X X X X X X X X X X X X X X X X X 36 92.30%  Introduction Sufficient scientific background X X X X X  X X X X  X X X X X X X X X X X X X X X X X X X X X X X X X X X X 37 94.87% Explanation of the experimental approach and rationale  X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X  X 37 94.87% Objectives Clear primary and second objectives X X X X   X X X X X  X X X X X X  X X X X   X X X X X X X X X  X X X X 32 82.05%  Material and methods Ethical statement Nature of the ethical review permissions and institutional guidelines for the care and use of animals  X X X X X X X X X X X X X X X X X X  X X  X X X X X X X X X X X X X X X X 36 92.3% Study design Number of animals per group  X X X   X X X X X X X X  X    X X   X X X X X X X  X X X X X X X X 29 74.35% Information on whether the experiment was performed as a blind controlled study     X   X               X    X      X    X   6 15.38% Experimental procedures Treatment X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X 39 100.00% Dosage of treatment X X X X X X X X X X X X  X X X X X X X X X X  X X X X X X X X X X X X X X X 37 94.87% Route of administration X X X X X X X X X X X X X X X X X  X X X X X X X X X X X X X X X X X X X X X 38 97.44% Duration of treatment X X X X  X X   X X X  X X X X X X X X X X X X X X X X X X X X X X X X X X 35 89.74% Time of day for treatment administration  X          X      X X X   X X       X     X    9 23.07% Location used for administration of treatment                            X            1 2.56% Rationale for choice of specific dosage     X  X  X   X X  X     X X  X          X   X   X 12 30.76% Rationale for choice of specific route of administration     X  X  X                           X  X X 6 15.38% Experimental animals Information on animal species X X X X X X X X X X X X X X  X X X X X X X X X X X X X X X X X X X   X X  35 89.74% Strain of the animals  X   X X X X X X X  X   X X X X X X X X X X X   X X X X X X   X X X 29 74.36% Sex of the animals X X X X X X X X X X X X X X X X X X  X X X X X X X X X X X  X X X  X X X X 36 92.30% Animals weight range  X     X     X  X  X X X  X X X  X X  X X X  X X X X   X X X 22 56.41% Age of the animals X  X X X X   X  X  X X X X   X X  X X  X X   X    X X X  X   22 56.41% Description of genetic modification status (knock-out, transgenic, and SPF) X  X X     X X X    X   X X   X X     X     X X  X X X X 18 46.15% Information related to previous procedures performed on the animals  X X X X X X X   X X X X X   X X X X X X X X  X       X   X X X 25 64.10% Housing and husbandry Housing of experimental animals (type of facility, type of cage or housing, material, and number of cage companions)   X    X X   X X X   X   X  X     X X X X        X   14 35.89% Husbandry conditions (breeding programme, light/dark cycle, and temperature of water)  X X X X  X X  X X X X X X X X  X X X X X X X X X X X X X X X X X  X  X 33 84.62% Welfare-related assessments and interventions that were carried out before, during, or after the experiment       X             X X  X              X   5 12.82% Sample size Total number of animals used in each experiment and the number of animals in each experimental group  X X X   X X X X  X X   X     X  X X X X X X X X  X   X  X   22 56.41% Explanation regarding the decision of the number of animals and details of sample size calculation       X                          X       2 5.12% Allocation of animals into experimental groups Full details of how animals were allocated to experimental groups (including randomization or matching)    X   X  X    X     X     X  X   X X   X     X   11 28.20% Order in which the animals in the different experimental groups were treated and assessed X X X X X  X X X  X X      X X X X X X X X X X X  X   X X X  X X X 28 71.79% Experimental outcomes Clear experimental outcomes assessed  X X X X  X X X X X X  X       X X X X X X X X X X X X X X   X X X 28 71.79% Statistical methods Statistical methods used for each analysis X X X X X  X X X X   X X X X X X X X X X X X X X X X X  X X X X X  X X X 34 87.18% Specification of the unit of analysis for each dataset    X X  X X X X  X X X X X X X X X X X X X X X X X X X X X X X X  X X X 33 84.61% Methods used to assess whether the data met the assumptions of the statistical approach X X X X X  X X X X  X X X  X X X X X X X X X X X X X X X X X X X   X X X 34 87.18%  Results Baseline data Description of the animals health status, for each experimental group, before treatment X X X X     X X        X X X X X X X   X  X   X  X   X   18 46.15% Number analyzed Number or animals in each group included in each analysis (absolute numbers)       X X  X      X          X X  X   X X X   X  X 12 30.76% Animals or data not included in the analysis (and explanation for the exclusion)                                  X      1 2.56% Outcomes and estimation Information (mean = standard deviation) X X X X X       X X     X X X X X X X X        X X   X X X 20 51.28% Information on quantification of inflammatory cells (mean = standard deviation)                        X                1 2.56% Adverse events Information on mortality of experimental animal (mean = standard deviation)   X                  X           X        3 7.69% Modifications to the experimental protocols made to reduce adverse events   X                                  X   2 5.12% Discussion Interpretation/scientific implications Interpretation of the results, taking into account the study objectives and hypotheses X X X X  X X X X X X X X X X  X X X X X X X X X X X X X X X X X X   X X X 35 89.74% Comments on the study limitations (sources of bias and imprecision associated with the results)  X X   X X  X X          X             X X   X X X 12 30.76% Generalizability /translation Comments on how the findings are likely to translate to other species or systems (relevance to human biology)   X X X X X X X X  X X  X X X X     X   X X      X X   X X X 22 56.41% Funding List of funding sources and the role of the funder(s) in the study  X X X      X   X X X X X  X X X  X X X X X X X X X X X X  X X X X 28 71.79%  Results 17 28 31 29 24 15 33 26 28 27 23 25 27 23 20 24 21 24 22 27 29 25 32 27 27 27 29 27 28 22 21 25 32 32 16 17 38 26 30


### 2.3. ARRIVE (Bias Analyses)

The detailed reports of experiments are crucial in the review process, so that they can be validated and used as a source of information for further research. However, many studies do not bring relevant or concise information, which leads to the realization of redundant and duplicated experiments [23]. Therefore, guidelines were developed for animal research reports, such as the ARRIVE guideline, based on the CONSORT Statement. The ARRIVE guidance is a list of 20 items that describe the minimum information that all scientific publications reporting research using animals must include, aiming at high quality reports and critical and accurate review of what was performed and found [24]. Thus, based on these fundamentals and the objective of this study, a table displaying the most relevant and applicable items from the ARRIVE was developed for a critical evaluation of the studies included in this review (Table 2). The authors assessed the quality, integrity, and transparency of each publication. Divergent opinions were resolved by consensus.


## 2.1. Selection of Papers

The papers analyzed in this review were selected from two electronic databases, PubMed and Scopus, accessed on September 3, 2015, using the search filters: “animal model”, “plant extract”, and “diabetes mellitus type II”. These filters have been developed for the search on PubMed, according to the Medical Subject Headings (MeSH terms), used for a more efficient indexing of publications on the subject under study [20]. In order to expand the search, MeSH terms were combined with the title and abstract (TIAB). A standard filter was used [21] to identify all studies with animals in PubMed. The terms used to search on PubMed were adapted for the selection of Scopus publications, and the “animal model” filter was provided by the site itself (Supplemental Data 1 in Supplementary Material available online at http://dx.doi.org/10.1155/2016/3537163).The Prism guideline was used to develop this review [22]. After the papers were collected from the two electronic databases, the duplicates were excluded by comparing the title, author, year, and country. A screening was performed for title and abstract, guided by the eligibility criteria:in vivo experimental studies; studies using rats or diabetic mice; use of fractions or isolates of noncommercial plants; treatment of the main symptoms of type II diabetes; studies written in English or Portuguese.Next, all selected papers were obtained in full for a second screening, when all of them were examined to select those that met the criteria for the inclusion in the systematic review. Those unavailable on the internet were requested from their respective authors. When they did not respond, the studies were excluded. The entire search process, exclusion, and the number of selected papers were described in detail in the PRISMA Guideline (Figure1).Figure 1
Results obtained after the advanced search in the databases. After literature review, 39 papers were selected from Moher D, Liberati A, Tetzlaff J, Altman DG, and the PRISMA Group (2009). Preferred reporting items for systematic reviews and meta-analyses: the PRISMA Statement. PLoS Med 6(6): e1000097. doi: 10.1371/journal.pmed1000097. For more information, visitwww.prisma-statement.org. Prism: systematic review.


## 2.2. Qualitative Characteristics of Publications

After screening, the papers were reviewed. Table2 shows the description of the main characteristics of the studies. The following parameters were assessed: (1) publication features: author, year, and country; (2) experimental features: animal model, species, sample number, sex, weight, age, type of caging used, number of animals per cage, number of experimental groups and number of animals in each group, if randomization was made, and control groups; (3) treatment features: plant species used, name of the fraction or isolate, dose, route of administration, and treatment duration; (4) diabetes induction: drug used, dose, route of administration, and testing to prove diabetes occurrence (Table 1).Table 1
Table of qualitative descriptors of the studies of the effects of fractions and isolates from the plants on the treatment of the type II diabetes.
(a) Title Author/publication year Country Isolates [25] A Polysaccharide Extract of Mulberry Leaf Ameliorates Hepatic Glucose Metabolism and Insulin Signaling In Rats with Type 2 Diabetes Induced by High Fat-Diet and Streptozotocin Ren et al. (2015) China [26] Antidiabetic Activity of a Xanthone Compound, Mangiferin Miura et al. (2001) Japan [27] Anti-Diabetic Effect of a Novel N-Trisaccharide Isolated from Cucumis prophetarumon Streptozotocin-Nicotinamide Induced Type 2 Diabetic Rats Kavishankar and Lakshmidevi (2014) India [28] Antidiabetic Effects of the Cimicifuga racemosa Extract Ze 450 In Vitro and In Vivo in ob/ob Mice Moser et al. (2014) Switzerland [29] Borapetoside C from Tinospora crispa Improves Insulin Sensitivity in Diabetic Mice Ruan et al. (2012) Taiwan [30] Dehydrotrametenolic Acid Induces Preadipocyte Differentiation and Sensitizes Animal Models of Noninsulin-Dependent Diabetes Mellitus to Insulin Sato et al. (2002) Japan [31] Effects of Ingested Fruiting Bodies, Submerged Culture Biomass, and Acidic Polysaccharide Glucuronoxylomannan of Tremella mesenterica Retz.:Fr. on Glycemic Responses in Normal and Diabetic Rats Lo et al. (2006) Taiwan [32] Inhibition of Glycogen Synthase Kinase-3β by Falcarindiol Isolated from Japanese Parsley (Oenanthe javanica) Yoshida et al. (2013) Japan [33] Isolation and Antihyperglycemic Activity of Bakuchiol from Otholobiumpubescens (Fabaceae), a Peruvian Medicinal Pant Used for the Treatment of Diabetes Krenisky et al. (1999) USA [34] LBP-4a Improves Insulin Resistance via Translocation and Activation of GLUT4 in OLETF Rats Zhao et al. (2014) China [35] Meliacinolin: A Potent Α-Glucosidase and Α-Amylase Inhibitor Isolated from Azadirachta indica Leaves and In Vivo Antidiabetic Property in Streptozotocin-Nicotinamide-Induced Type 2 Diabetes in Mice Perez-Gutierrez and Damian-Guzman (2012) Mexico [36] Novel Terpenoid-Type Quinones Isolated from Pycnanthus angolensis of Potential Utility in the Treatment of Type 2 Diabetes Luo et al. (1999) USA [37] Platyconic Acid, A Saponin from Platycodi radix, Improves Glucose Homeostasis by Enhancing Insulin Sensitivity In Vitro and In Vivo Kwon et al. (2012) South Korea [38] Rhaponticin from rhubarb Rhizomes Alleviates Liver Steatosis and Improves Blood Glucose and Lipid Profiles in KK/Ay Diabetic Mice Chen et al. (2009) China [39] Rutin Potentiates Insulin Receptor Kinase to Enhance Insulin-Dependent Glucose Transporter 4 Translocation Hsu et al. (2014) Taiwan [40] Type 2 Antidiabetic Activity of Bergenin from the Roots of Caesalpinia digynaRottler Kumar et al. (2012) India  Fractions [41] A Study on Hypoglycaemic Health Care Function of Stigma maydis Polysaccharides Zhang et al. (2013) China [1] Antidiabetic Activities of Extract from Malva verticillataSeed via the Activation of AMP-Activated Protein Kinase Jeong and Song (2011) Korea [42] Antidiabetic Activity of Alkaloids of Aerva lanata Roots on Streptozotocin-Nicotinamide Induced Type-II Diabetes in Rats Agrawal et al. (2013) India [43] Antidiabetic Activity of Pterospermum acerifolium Flowers and Glucose Uptake Potential of Bioactive Fraction in L6 Muscle Cell Lines with Its HPLC Fingerprint Paramaguru et al. (2014) India [44] Antidiabetic Activity of Caesalpinia bonducella F. in Chronic Type 2 Diabetic Model in Long-Evans Rats and Evaluation of Insulin Secretagogue Property of Its Fractions on Isolated Islets Chakrabarti et al. (2005) India [45] Antidiabetic Effect of an Acidic Polysaccharide (TAP) from Tremella aurantiaand Its Degradation Product (TAP-H) Kiho et al. (2001) Japan [46] Antidiabetic Effect of Orally Administered Conophylline-Containing Plant Extract on Streptozotocin-Treated and Goto-Kakizaki Rats Fujii et al. (2009) Japan [47] Antidiabetic Effect of Total Flavonoids from Sanguisd raxonis  in Type 2 Diabetic Rats Chen et al. (2013) China [48] Antidiabetic Effects of Bitter Gourd Extracts in Insulin-Resistant db/db Mice Klomann et al. (2010) Germany [49] Anti-Diabetic Effects of Centratherum anthelminticum Seeds Methanolic Fraction on Pancreatic Cells, Β-TC6 and Its Alleviating Role in Type 2 Diabetic Rats Arya et al. (2012) Malaysia [17] Anti-Diabetic Effects of Polysaccharides from Talinum triangulare in Streptozotocin (STZ)-Induced Type 2 Diabetic Male Mice Xu et al. (2015) China [50] Anti-Diabetic Effects of the Acetone Fraction of Senna singueana Stem Bark in a Type 2 Diabetes Rat Model Ibrahim and Islam (2014) South Africa [51] Antidiabetic Potential of Polysaccharides from the White Oyster Culinary-Medicinal Mushroom Pleurotus florida (Higher Basidiomycetes) Ganeshpurkar et al. (2014) India [52] Antihyperglycemic Effects of Total Flavonoids from Polygonatum odoratum in STZ and Alloxan-Induced Diabetic Rats Shu et al. (2009) China [53] Antioxidant and Anti-Inflammatory Effects of a Hypoglycemic Fraction from Cucurbita ficifolia  Bouché in Streptozotocin-Induced Diabetic Mice Roman-Ramos et al. (2012) Mexico [54] Effect of Lycium barbarumPolysaccharide on the Improvement of Insulin Resistance in NIDDM Rats Zhao et al. (2005) China [55] Effects of Grape Seed Extract and Its Ethylacetate/Ethanol Fraction on Blood Glucose Levels in a Model of Type 2 Diabetes Hwang et al. (2009) South Korea [56] Hypoglycemic Effect of Astragaluspolysaccharide and Its Effect on PTP1B1 Wu et al. (2005) China [57] Hypoglycemic Effects of MDG-1, a Polysaccharide Derived from Ophiopogonjaponicus, in the ob/ob Mouse Model of Type 2 Diabetes Mellitus Xu et al. (2011) China [58] Isolation and Pharmacological Activities of the Tecoma stans Alkaloids Costantino et al. (2003) Italy [58] Potent Effects of the Total Saponins From Dioscorea nipponica Makino against Streptozotocin-Induced Type 2 Diabetes Mellitus in Rats Yu et al. (2015) China [59] Tocopherol from Seeds of Cucurbita pepo against Diabetes: Validation by In Vivo Experiments Supported by Computational Docking Bharti et al. (2013) India [60] Trapa natans L. Root Extract Suppresses Hyperglycemic and Hepatotoxic Effects in STZ-Induced Diabetic Rat Model Kharbanda et al. (2014) India  (b) Title Animal model/strain Number of animals Sex Age Weight Housing of animals Animals per cage Groups and number of animals in each group Randomization Isolates [25] A Polysaccharide Extract of Mulberry Leaf Ameliorates Hepatic Glucose Metabolism and Insulin Signaling in Rats with Type 2 Diabetes Induced by High Fat-Diet and Streptozotocin Wistar rats 18 M 7 w 200 g Cages 5 3 groups (6 in each group) Yes [26] Antidiabetic Activity of a Xanthone Compound, Mangiferin Mice ? M 12 w ? ? ? ? ? [27] Anti-Diabetic Effect of a Novel N-Trisaccharide Isolated from Cucumis prophetarumon Streptozotocin-Nicotinamide Induced Type 2 Diabetic Rats Wistar rats 36 M&F ? 150–180 g Cages ? 6 groups (6 animals in each) ? [28] Antidiabetic Effects of the Cimicifuga racemosa Extract Ze 450 In Vitro and In Vivo in ob/ob Mice ob/ob mice 68 M 7-8 w ? Cages 1 8 groups (8 animals in each) ? [29] Borapetoside C from Tinospora crispa Improves Insulin Sensitivity in Diabetic Mice Mice ? M 8–10 w ? ? ? ? ? [30] Dehydrotrametenolic Acid Induces Preadipocyte Differentiation and Sensitizes Animal Models of Noninsulin-Dependent Diabetes Mellitus to Insulin C57BLK mice ? M 7 w ? ? ? ? ? [31] Effects of Ingested Fruiting Bodies, Submerged Culture Biomass, and Acidic Polysaccharide Glucuronoxylomannan of Tremella mesenterica Retz.:Fr. on Glycemic Responses in Normal and Diabetic Rats Wistar rats 102 M ? 200 g Cages 1 6 groups (12 animals in each group) and 3 groups (10 animals in each group) Yes [32] Inhibition of Glycogen Synthase Kinase-3β by Falcarindiol Isolated from Japanese Parsley (Oenanthe javanica) GK rats 12 M ? ? Cages 1 2 groups (6 animals in each group) ? [33] Isolation and Antihyperglycemic Activity of Bakuchiol from Otholobiumpubescens (Fabaceae), a Peruvian Medicinal Plant Used for the Treatment of Diabetes C57BL/K mice & Sprague- Dawley rats ? M 8 w ? ? 4 ? Groups (8 animals in each group) ? [34] LBP-4a Improves Insulin Resistance via Translocation and Activation of GLUT4 in OLETF Rats Otsuka Long-Evans Tokushima Fatty (OLETF) rats 18 M 26 w ? ? ? 3 groups (6 animals in each group) Yes [35] Meliacinolin: A Potent Α-Glucosidase and Α-Amylase Inhibitor Isolated from Azadirachta indica Leaves and In Vivo Antidiabetic Property in Streptozotocin-Nicotinamide-Induced Type 2 Diabetes in Mice ?/mice 42 M ? 20–25 g Cages 2 7 groups (6 animals in each group) ? [36] Novel Terpenoid-Type Quinones Isolated from Pycnanthus angolensis of Potential Utility in the Treatment of Type 2 Diabetes C57BLK mice ? M 7-8 w ? Cages 4 ? groups (5–8 animals in each group) ? [37] Platyconic Acid, a Saponin from Platycodi radix, Improves Glucose Homeostasis by Enhancing Insulin Sensitivity In Vitro and In Vivo C57BLK mice 90 M 8–10 w ? ? 1 6 groups (15 animals in each group) Yes [38] Rhaponticin from rhubarb Rhizomes Alleviates Liver Steatosis and Improves Blood Glucose and Lipid Profiles in KK/Ay Diabetic Mice Mice 30 M&F 8–12 w ? Cages 1 3 groups (10 animals in each group) Yes [39] Rutin Potentiates Insulin Receptor Kinase to Enhance Insulin-Dependent Glucose Transporter 4 Translocation Mice ? M 8 w ? ? ? ? ? [40] Type 2 Antidiabetic Activity of Bergenin from the Roots of  Caesalpinia digyna Rottler Charles Foster Albino rats 30 M ? ? ? ? 5 groups (6 animals in each group) ?  Fractions [41] A Study on Hypoglycaemic Health Care Function of Stigma maydis Polysaccharides SPF km mice ? M ? 20 ± 2 g ? ? 7 groups (animals in each group?) Yes [1] Antidiabetic Activities of Extract from Malva verticillataSeed via the Activation of AMP-Activated Protein Kinase. C57BLK mice ? ? 5 w ? Cages 5 5 groups (animals in each group?) ? [42] Antidiabetic Activity of Alkaloids of Aerva lanata Roots on Streptozotocin-Nicotinamide Induced Type-II Diabetes in Rats Wistar rats ? M ? 200–250 g ? ? ? ? [43] Antidiabetic Activity of Pterospermum acerifoliumFlowers and Glucose Uptake Potential of Bioactive Fraction in L6 Muscle Cell Lines with Its HPLC Fingerprint Albino Wistar rats 66 M ? 180–200 g Cages ? 11 groups (6 animals in each group) ? [44] Antidiabetic Activity of Caesalpinia bonducella F. in Chronic Type 2 Diabetic Model in Long-Evans Rats and Evaluation of Insulin Secretagogue Property of Its Fractions on Isolated Islets Long-Evans rats ? M&F 12–14 w 150 g ? ? 5 groups (6–9 animals in each group) ? [45] Antidiabetic Effect of an Acidic Polysaccharide (TAP) from Tremella aurantiaand Its Degradation Product (TAP-H) KK- Ay-TA mice ? M 5 w 25–30 g ? 1 3 groups (animals in each group?) ? [46] Antidiabetic Effect of Orally Administered Conophylline-Containing Plant Extract on Streptozotocin-Treated and Goto-Kakizaki Rats Goto-Kakizaki rats 14 M 5 w ? ? ? 3 groups (animals in each group?) Yes [47] Antidiabetic Effect of Total Flavonoids from Sanguis draxonis  in Type 2 Diabetic Rats Sprague-Dawley rats 72 M ? 180–220 g ? ? 6 groups (6 animals in each group) for SD; 6 groups (6 animals in each group) for SDF ? [48] Antidiabetic Effects of Bitter Gourd Extracts in Insulin-Resistant db/db Mice db/db mice 45 M 5 w ? Cages 1 5 groups (9 animals in each group) ? [49] Anti-Diabetic Effects of Centratherum anthelminticum Seeds Methanolic Fraction on Pancreatic Cells, Β-TC6 and Its Alleviating Role in Type 2 Diabetic Rats Sprague-Dawley rats 72 M&F ? 180–200 g ? ? 12 groups (6 animals in each group) ? [17] Anti-Diabetic Effects of Polysaccharides from Talinum triangulare in Streptozotocin (STZ)-Induced Type 2 Diabetic Male Mice SPF km mice 50 M ? 20 ± 2 g ? ? 5 groups (10 animals in each group) Yes [50] Anti-Diabetic Effects of the Acetone Fraction of Senna singueana Stem Bark in a Type 2 Diabetes Rat Model Sprague-Dawley rats 48 M 6 w 207.60 ± 4.27 g Cage 2 6 groups (8 animals in each group) Yes [51] Antidiabetic Potential of Polysaccharides from the White Oyster Culinary-Medicinal Mushroom Pleurotus florida (Higher Basidiomycetes) Wistar rats 20 M ? 150–200 g ? ? 4 groups (5 animals in each group) ? [52] Antihyperglycemic Effects of Total Flavonoids from Polygonatum odoratum in STZ and Alloxan-Induced Diabetic Rats Sprague-Dawley rats ? ? ? 220 ± 4.5 g Special animal house ? ? ? [53] Antioxidant and Anti-Inflammatory Effects of a Hypoglycemic Fraction from Cucurbita ficifolia  Bouché in Streptozotocin-Induced Diabetic Mice CD-1 mice ? M ? 30–35 g ? ? ? ? [54] Effect of Lycium barbarumPolysaccharide on the Improvement of Insulin Resistance in NIDDM Rats Wistar rats 40 M ? 230–250 g Cages ? ? Yes [55] Effects of Grape Seed Extract and Its Ethylacetate/Ethanol Fraction on Blood Glucose Levels in a Model of Type 2 Diabetes C57BL/KsJ-leprdb/leprdb mice 98 M 3 w 9.7–14.2 g Conventional state ? 7 groups (14 in each group) ? [56] Hypoglycemic Effect of Astragaluspolysaccharide and Its Effect on PTP1B1 Sprague-Dawley rat 34 M 8 w 200 g ? 5 4 groups (2 groups with 10 animals and 2 groups with 12 animals in each) ? [57] Hypoglycemic Effects of MDG-1, a Polysaccharide Derived from Ophiopogonj aponicus, in the ob/ob Mouse Model of Type 2 Diabetes Mellitus ob/ob mice ?  6-7 w ? ? ? 4 groups (8 animals in each group) ? [58] Isolation and Pharmacological Activities of the Tecoma stans Alkaloids C57BL/KsJ db/db mice ? M 8 w ? ? ? ? ? [61] Potent Effects of the Total Saponins from Dioscorea nipponica Makino against Streptozotocin-Induced Type 2 Diabetes Mellitus in Rats Wistar rats 70 M ? 190–200 g Cages 1 7 groups (8 animals in each group) Yes [59] Tocopherol from Seeds of Cucurbita pepo against Diabetes: Validation by In Vivo Experiments Supported by Computational Docking Albino Wistar rats 24 M ? 150–160 g ? ? 4 groups (6 animals in each group) ? [60] Trapa natans L. Root Extract Suppresses Hyperglycemic and Hepatotoxic Effects in STZ-Induced Diabetic Rat Model Albino Wistar rats 90 ? ? 150–200 g Cages ? 15 groups (6 animals in each group) ?  (c) Title Plant species Isolate/fraction Administration Doses used Duration of treatment Isolates [25] A Polysaccharide Extract of Mulberry Leaf Ameliorates Hepatic Glucose Metabolism and Insulin Signaling in Rats with Type 2 Diabetes Induced by High Fat-Diet and Streptozotocin Mulberry Mulberry leaf polysaccharide Gavage 200 mg/kg 6 days of treatment [26] Antidiabetic Activity of a Xanthone Compound, Mangiferin Anemarrhena asphodeloidesBunge Mangiferin Orally 30 mg/kg ? [27] Anti-Diabetic Effect of a Novel N-Trisaccharide Isolated from Cucumis prophetarum on Streptozotocin-Nicotinamide Induced Type 2 Diabetic Rats Cucumis prophetarum N-Trisaccharide Gavage 50, 5 mg/kg 28 days of treatment [28] Antidiabetic Effects of the Cimicifuga racemosa Extract Ze 450 In Vitro and In Vivo in ob/ob Mice Cimicifuga racemosa Ze 450 Gavage 10, 30, 90 mg/kg 7 days of treatment [29] Borapetoside C from Tinospora crispa Improves Insulin Sensitivity in Diabetic Mice Tinospora crispa Borapetoside C Orally 5 mg/kg 4 weeks of treatment [30] Dehydrotrametenolic Acid Induces Preadipocyte Differentiation and Sensitizes Animal Models of Noninsulin-Dependent Diabetes Mellitus to Insulin Poria cocosWolf Dehydrotrametenolic acid Gavage 110 mg/kg 14 days of treatment [31] Effects of Ingested Fruiting Bodies, Submerged Culture Biomass, and Acidic Polysaccharide Glucuronoxylomannan of Tremella mesenterica Retz.:Fr. on Glycemic Responses in Normal and Diabetic Rats Tremella mesenterica Acidic polysaccharide glucuronoxylomannan (GXM) Gavage 1 g/kg 15 days of treatment [32] Inhibition of Glycogen Synthase Kinase-3β by Falcarindiol Isolated from Japanese Parsley (Oenanthe javanica) Oenanthe javanica falcarindiol Orally 15 mg/kg ? [33] Isolation and Antihyperglycemic Activity of Bakuchiol from Otholobiumpubescens (Fabaceae), a Peruvian Medicinal Plant Used for the Treatment of Diabetes Otholobium pubescens Bakuchiol Gavage 1, 150, 250 mg/kg 2 weeks of treatment [34] LBP-4a Improves Insulin Resistance via Translocation and Activation of GLUT4 in OLETF Rats Lycium barbarum Lycium barbarum polysaccharide (LBP-4a) Orally 10 mg/kg 4 weeks of treatment [35] Meliacinolin: A Potent Α-Glucosidase and Α-Amylase Inhibitor Isolated from Azadirachta indica Leaves and In Vivo Antidiabetic Property in Streptozotocin-Nicotinamide-Induced Type 2 Diabetes in Mice Azadirachta indica Meliacinolin Orally by gastric intubations 20 mg/kg 28 days of treatment [36] Novel Terpenoid-Type Quinones Isolated from Pycnanthus angolensis of Potential Utility in the Treatment of Type 2 Diabetes Pycnanthus angolensis Novel terpenoid-type quinones (SP-18904 and SP-18905) Gavage 100 mg/kg 4 days of treatment [37] Platyconic Acid, a Saponin from Platycodi radix, Improves Glucose Homeostasis by Enhancing Insulin Sensitivity In Vitro and In Vivo Platycodi radix Platyconic acid (PA), platycodin D (PD), platycoside E (PE), and saponin with low activity (DPE) Gavage 20 mg/kg 8 weeks of treatment [38] Rhaponticin from rhubarb Rhizomes Alleviates Liver Steatosis and Improves Blood Glucose and Lipid Profiles in KK/Ay Diabetic Mice Rheum franzenbachiiMunt Rhaponticin Orally 125 mg/kg 4 weeks of treatment [39] Rutin Potentiates Insulin Receptor Kinase to Enhance Insulin-Dependent Glucose Transporter 4 Translocation Toona sinensis Flavonoid rutin Gavage 25 mg/kg ? [40] Type 2 Antidiabetic Activity of Bergenin from the Roots of Caesalpinia digyna Rottler Caesalpinia digynaRottler Bergenin Orally 2.5, 5, and 10 mg/kg 14 days of treatment  Fractions [41] A Study on Hypoglycaemic Health Care Function of Stigma maydis Polysaccharides Stigma maydis Stigma maydis polysaccharides ? 20 mg/kg 4 weeks of treatment [1] Antidiabetic Activities of Extract from Malva verticillata Seed via the Activation of AMP-Activated Protein Kinase Malva verticillata Ethanol extract ofM. verticillata and N-hexane (MVE-H) Orally 3 different concentrations of MVE-H (10, 20, or 40 mg/kg) 4 weeks of treatment [42] Antidiabetic Activity of Alkaloids of Aerva lanata Roots on Streptozotocin-Nicotinamide Induced Type-II Diabetes in Rats Aerva lanataLinn. The partially purified alkaloid basified toluene fraction (PPABTF) Orally 10, 20 mg/kg 2 weeks of treatment [43] Antidiabetic Activity of Pterospermum acerifolium Flowers and Glucose Uptake Potential of Bioactive Fraction in L6 Muscle Cell Lines with Its HPLC Fingerprint Pterospermum acerifolium Ethyl acetate fraction (PAFEF) and subfractions PAFE1, PAFE2, and e PAFE3 Intragastric tube 200, 400 mg/kg, and 15, 30 mg/kg 30 days of treatment [44] Antidiabetic Activity of Caesalpinia bonducella F. in Chronic Type 2 Diabetic Model in Long-Evans Rats and Evaluation of Insulin Secretagogue Property of Its Fractions on Isolated Islets Caesalpinia bonducellaF. Caesalpinia bonducellaaqueous and alcoholic extracts (BM-170 and BM-171) Orally 250 mg/kg 28 days of treatment [45] Antidiabetic Effect of an Acidic Polysaccharide (TAP) from Tremella aurantiaand Its Degradation Product (TAP-H) Tremella aurantia Acidic polysaccharide (TAP) and the degradation product (TAP-H) Orally 0.5 g/L, 1.5 g/L 10 weeks of treatment [46] Antidiabetic Effect of Orally Administered Conophylline-Containing Plant Extract on Streptozotocin-Treated and Goto-Kakizaki Rats Tabernaemontana divaricata The Crude Conophylline Preparation I (CCP-I) Orally 200, 50 g/kg 15 days of treatment [47] Antidiabetic Effect of Total Flavonoids from Sanguis draxonis  in Type 2 Diabetic Rats Dracaena cochinchinensis Sanguis  draxonis (SD) and total flavonoids from SD (SDF) Gavage ? 21 days of treatment [48] Antidiabetic Effects of Bitter Gourd Extracts in Insulin-Resistant db/db Mice Momordica charantia The lipid fraction, the saponin fraction, or the hydrophilic residue of bitter gourd Orally 150 mg/kg 5 weeks of treatment [49] Anti-Diabetic Effects of Centratherum anthelminticum Seeds Methanolic Fraction on Pancreatic Cells, Β-TC6 and Its Alleviating Role in Type 2 Diabetic Rats Centratherum anthelminticum Crude methanolic fraction (CAMF) Injected intraperitoneally (IP) or orally 50 and 100 mg/kg 4 weeks of treatment [17] Anti-Diabetic Effects of Polysaccharides from Talinum triangulare in Streptozotocin (STZ)-Induced Type 2 Diabetic Male Mice Talinum triangulare Polysaccharides obtained fromTalinum triangulare (TTP) Orally 150 and 300 mg/kg 2 weeks of treatment [50] Anti-Diabetic Effects of the Acetone Fraction of Senna singueana Stem Bark in a Type 2 Diabetes Rat Model Senna singueana Senna singueana acetone fraction (SSAF) Orally 150 mg/kg and 300 mg/kg 4 weeks of treatment [51] Antidiabetic Potential of Polysaccharides from the White Oyster Culinary-Medicinal Mushroom Pleurotus florida (Higher Basidiomycetes) Pleurotus florida P. florida polysaccharides (PFPs) Orally 200 and 400 mg/kg 21 days of treatment [52] Antihyperglycemic Effects of Total Flavonoids from Polygonatum odoratum in STZ and Alloxan-Induced Diabetic Rats Polygonatum odoratum Total flavonoids ofPolygonatum (P) odoratum (TFP) Orally 50, 100, and 200 mg/kg 30 days of treatment [53] Antioxidant and Anti-Inflammatory Effects of a Hypoglycemic Fraction from Cucurbita ficifolia  Bouché in Streptozotocin-Induced Diabetic Mice Cucurbita ficifolia Aqueous-precipitate fraction (AP-fraction) Gavage 200 mg/kg 15 days of treatment [54] Effect of Lycium barbarumPolysaccharide on the Improvement of Insulin Resistance in NIDDM Rats Lycium barbarum Lycium barbarum polysaccharide (LBP) Orally 10 mg/kg 3 weeks of treatment [55] Effects of Grape Seed Extract and Its Ethylacetate/Ethanol Fraction on Blood Glucose Levels in a Model of Type 2 Diabetes Vitis viniferagrape Grape seed extract (GSE), ethylacetate (e), and ethylacetate/ethanol (ee) Orally 50 and 30 mg/kg 8 weeks of treatment [56] Hypoglycemic Effect of Astragaluspolysaccharide and Its Effect on PTP1B1 Astragalus membranaceus Astragalus polysaccharide (APS) Orally 400 mg/kg 5 weeks of treatment [57] Hypoglycemic Effects of MDG-1, a Polysaccharide Derived from Ophiopogon japonicus, in the ob/ob Mouse Model of Type 2 Diabetes Mellitus Ophiopogon japonicus Water-solubleβ-d-fructan (MDG-1) Intragastrically 150 and 300 mg/kg 23 days of treatment [58] Isolation and Pharmacological Activities of the Tecoma stans Alkaloids Tecoma stans Tecoma stans alkaloids Gavage 50 mg/kg and 63.4 mg/kg 7 days of treatment [61] Potent Effects of the Total Saponins from Dioscorea nipponica Makino against Streptozotocin-Induced Type 2 Diabetes Mellitus in Rats Dioscorea nipponica Saponins fromD. nipponicaMakino (TSDN) Orally 200, 100, and 50 mg/kg 12 weeks of treatment [59] Tocopherol from Seeds of Cucurbita pepo against Diabetes: Validation by In Vivo Experiments Supported by Computational Docking Cucurbita pepo Tocopherol ? 2, 5 g/kg 6 weeks of treatment [60] Trapa natans L. Root Extract Suppresses Hyperglycemic and Hepatotoxic Effects in STZ-Induced Diabetic Rat Model Trapa natansL. Methanol fraction, chloroform fraction, and petroleum ether fraction Orally 50, 100, and 200 mg/kg 15 days of treatment  (d) Title Drug for diabetes induction Route of induction Hyperglycemia Control of glycemia Insulin tolerance test Isolates [25] A Polysaccharide Extract of Mulberry Leaf Ameliorates Hepatic Glucose Metabolism and Insulin Signaling in Rats with Type 2 Diabetes Induced by High Fat-Diet and Streptozotocin Diet with 41.2% fat and a low-dose STZ (35 mg/kg body weight) Intraperitoneal Glucose ≥ 7.8 mmol/L ? Yes [26] Antidiabetic Activity of a Xanthone Compound, Mangiferin Genetically modified — — ? Yes [27] Anti-Diabetic Effect of a Novel N-Trisaccharide Isolated from Cucumis prophetarum on Streptozotocin-Nicotinamide Induced Type 2 Diabetic Rats Nicotinamide (NA) at 230 mg/kg and STZ at 65 mg/kg Intraperitoneal Glucose ≥ 250 mg/dL Yes Yes [28] Antidiabetic Effects of the Cimicifuga racemosa Extract Ze 450 In Vitro and In Vivo in ob/ob Mice Genetically modified — — ? ? [29] Borapetoside C from Tinospora crispa Improves Insulin Sensitivity in Diabetic Mice STZ 150 mg/kg Intraperitoneal Glucose ≥ 150 mg/dL Yes Yes [30] Dehydrotrametenolic Acid Induces Preadipocyte Differentiation and Sensitizes Animal Models of Noninsulin-Dependent Diabetes Mellitus to Insulin Genetically modified — — ? ? [31] Effects of Ingested Fruiting Bodies, Submerged Culture Biomass, and Acidic Polysaccharide Glucuronoxylomannan of Tremella mesenterica Retz.:Fr. on Glycemic Responses in Normal and Diabetic Rats STZ (65 mg/kg) and nicotinamide (200 mg/kg) Intraperitoneal Glucose > 250 mg/100 mL Yes Yes [32] Inhibition of Glycogen Synthase Kinase-3β by Falcarindiol Isolated from Japanese Parsley (Oenanthe javanica) Genetically modified — — ? Yes [33] Isolation and Antihyperglycemic Activity of Bakuchiol from Otholobiumpubescens (Fabaceae), a Peruvian Medicinal Plant Used for the Treatment of Diabetes STZ 50 mg/kg Intravenous Glucose 300–600 mg/dL Yes ? [34] LBP-4a Improves Insulin Resistance via Translocation and Activation of GLUT4 in OLETF Rats Genetically modified — — ? Yes [35] Meliacinolin: A Potent Α-Glucosidase and Α-Amylase Inhibitor Isolated from Azadirachta indica Leaves and In Vivo Antidiabetic Property in Streptozotocin-Nicotinamide-Induced Type 2 Diabetes in Mice Nicotinamide (120 mg/kg) and STZ (60 mg/kg) Intraperitoneal Glucose > 250 mg/dL Yes ? [36] Novel Terpenoid-Type Quinones Isolated from Pycnanthus angolensis of Potential Utility in the Treatment of Type 2 Diabetes STZ 150 mg/kg Intravenous Glucose 300–600 mg/dL Yes Yes [37] Platyconic Acid, a Saponin from Platycodi radix, Improves Glucose Homeostasis by Enhancing Insulin Sensitivity In Vitro and In Vivo STZ 20 mg/kg ? ? Yes Yes [38] Rhaponticin from rhubarb Rhizomes Alleviates Liver Steatosis and Improves Blood Glucose and Lipid Profiles in KK/Ay Diabetic Mice STZ/? ? ? Yes ? [39] Rutin Potentiates Insulin Receptor Kinase to Enhance Insulin-Dependent Glucose Transporter 4 Translocation Insulin receptor antagonist S960 (50 nmol/kg) Intravenous injection ? Yes ? [40] Type 2 Antidiabetic Activity of Bergenin from the Roots of Caesalpinia digyna Rottler STZ (65 mg/kg) and nicotinamide (110 mg/kg) Intraperitoneal injection Glucose 200 mg/dL Yes Yes  Fractions [41] A Study on Hypoglycaemic Health Care Function of Stigma maydis Polysaccharides High fat-diet and low-dose alloxan (90 mg/kg) Intraperitoneal Glucose ≥ 10 mmol/L Yes ? [1] Antidiabetic Activities of Extract from Malva verticillata Seed via the Activation of AMP-Activated Protein Kinase Genetically modified — — Yes ? [42] Antidiabetic Activity of Alkaloids of Aerva lanata Roots on Streptozotocin-Nicotinamide Induced Type-II Diabetes in Rats STZ and nicotinamide Intraperitoneal ? Yes ? [43] Antidiabetic Activity of Pterospermum acerifolium Flowers and Glucose Uptake Potential of Bioactive Fraction in L6 Muscle Cell Lines with Its HPLC Fingerprint STZ (60 mg/kg) and nicotinamide (120 mg/kg) Intraperitoneal Glucose > 250 mg/dL Yes ? [44] Antidiabetic Activity of Caesalpinia bonducella F. in Chronic Type 2 Diabetic Model in Long-Evans Rats and Evaluation of Insulin Secretagogue Property of Its Fractions on Isolated Islets STZ 90 mg/kg Intraperitoneal injection Glucose 7–12 mmol/L Yes Yes [45] Antidiabetic Effect of an Acidic Polysaccharide (TAP) from Tremella aurantiaand Its Degradation Product (TAP-H) Genetically modified — — ? Yes [46] Antidiabetic Effect of Orally Administered Conophylline-Containing Plant Extract on Streptozotocin-Treated and Goto-Kakizaki Rats Streptozotocin 60 mg/kg Intraperitoneal Glucose > 250 mg/dL Yes ? [47] Antidiabetic Effect of Total Flavonoids from Sanguis draxonis  in Type 2 Diabetic Rats High fat-diet and a singular injection of streptozotocin (STZ) (35 mg/kg) Intraperitoneal Glucose ≥ 11.1 mmol/L Yes Yes [48] Antidiabetic Effects of Bitter Gourd Extracts in Insulin-Resistant db/db Mice Genetically modified — — ? ? [49] Anti-Diabetic Effects of Centratherum anthelminticum Seeds Methanolic Fraction on Pancreatic Cells, Β-TC6 and Its Alleviating Role in Type 2 Diabetic Rats Streptozotocin (STZ) (65 mg/kg) Intraperitoneal Glucose 22–26 mmol/L Yes ? [17] Anti-Diabetic Effects of Polysaccharides from Talinum triangulare in Streptozotocin (STZ)-Induced Type 2 Diabetic Male Mice Streptozotocin (STZ) (70 mg/kg) Intraperitoneal ? Yes ? [50] Anti-Diabetic Effects of the Acetone Fraction of Senna singueana Stem Bark in a Type 2 Diabetes Rat Model STZ 40 mg/kg Intraperitoneal injection Glucose > 18 mmol/L Yes ? [51] Antidiabetic Potential of Polysaccharides from the White Oyster Culinary-Medicinal Mushroom Pleurotus florida (Higher Basidiomycetes) STZ 50 mg/kg Intraperitoneal Glucose > 200 mg/100 mL Yes ? [52] Antihyperglycemic Effects of Total Flavonoids from Polygonatum odoratum in STZ and Alloxan-Induced Diabetic Rats Alloxan (100 and 120 mg/kg) Intraperitoneal Glucose > 11.0 mmol/L Yes ? [53] Antioxidant and Anti-Inflammatory Effects of a Hypoglycemic Fraction from Cucurbita ficifoliaBouché in Streptozotocin-Induced Diabetic Mice STZ 137 mg/kg Intraperitoneal ? ? ? [54] Effect of Lycium barbarumPolysaccharide on the Improvement of Insulin Resistance in NIDDM Rats STZ 50 mg/kg Intraperitoneal Glucose > 16 mmol/L Yes Yes [55] Effects of Grape Seed Extract and Its Ethylacetate/Ethanol Fraction on Blood Glucose Levels in a Model of Type 2 Diabetes Genetically modified — — Yes ? [56] Hypoglycemic Effect of Astragalus Polysaccharide and Its Effect on PTP1B1 STZ 30 mg/kg Intravenous injection Glucose > 6.7 mmol/L Yes Yes [57] Hypoglycemic Effects of MDG-1, a Polysaccharide Derived from Ophiopogonjaponicus, in the ob/ob Mouse Model of Type 2 Diabetes Mellitus Genetically diabetic model — — Yes Yes [58] Isolation and Pharmacological Activities of the Tecoma stans Alkaloids Genetically diabetic model — — ? Yes [61] Potent Effects of the Total Saponins from Dioscorea nipponica Makino against Streptozotocin-Induced Type 2 Diabetes Mellitus in Rats STZ 30 mg/kg Intraperitoneal Glucose > 16.7 mmol/L Yes Yes [59] Tocopherol from Seeds of Cucurbita pepo against Diabetes: Validation by In Vivo Experiments Supported by Computational Docking PX-407 solution (10 mg/kg) ? Blood glucose level of 200 mg/dL or higher Yes Yes [60] Trapa natans L. Root Extract Suppresses Hyperglycemic and Hepatotoxic Effects in STZ-Induced Diabetic Rat Model STZ (45 mg/kg) Intraperitoneal Glucose > 200 mg/dL Yes ?Table 2
Biases analyses (ARRIVE) of the studies of the effects of fractions and isolates from the plants in the treatment of the type II diabetes.
Isolates Fractions References Miura, et al., 2001 [26] Kavishankar and Lakshmidevi, 2014 [27] Moser et al., 2014 [28] Chen et al., 2009 [38] Ruan et al., 2012 [29] Sato et al., 2002 [30] Lo et al., 2006 [31] Yoshida et al., 2013 [32] Zhao et al., 2014 [34] Kumar et al., 2012 [40] Luo et al., 1999 [36] Perez-Gutierrez and Damian-Guzman, 2012 [35] Kwon et al., 2012 [37] Krenisky et al., 1999 [33] Hsu  et al., 2014 [39] Ren et al., 2015 [25] Agrawal et al., 2013 [42] Zhang et al., 2013 [41] Jeong and Song, 2011 [1] Chakrabarti et al., 2005 [44] Paramaguru et al., 2014 [43] Kiho et al., 2001 [45] Fujii et al., 2009 [46] Chen et al., 2013 [47] Arya et al., 2012 [49] Klomann et al., 2010 [48] Arya et al., 2012 [49] Xu et al., 2015 [17] Ibrahim and Islam, 2014 [50] Ganeshpurkar et al., 2014 [51] Shu et al., 2009 [52] Zhao et al., 2005 [54] Hwang et al., 2009 [55] Wu et al., 2005 [56] Xu et al., 2011 [57] Costantino et al., 2003 [58] Yu et al., 2015 [61] Kharbanda et al., 2014 [60] Roman-Ramos et al., 2012 [53] Accurate and concise description of the content of the article  X X X X X X X X X X  X X  X X   X X  X X X X X X X X X X X X X X X X X 33 84.62%  Abstract Summary of the background, research objectives, methods, main findings, and conclusions X X X X X  X X X X X X X X X X X X X   X X X X X X X X X X X X X X X X X X 36 92.30%  Introduction Sufficient scientific background X X X X X  X X X X  X X X X X X X X X X X X X X X X X X X X X X X X X X X X 37 94.87% Explanation of the experimental approach and rationale  X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X  X 37 94.87% Objectives Clear primary and second objectives X X X X   X X X X X  X X X X X X  X X X X   X X X X X X X X X  X X X X 32 82.05%  Material and methods Ethical statement Nature of the ethical review permissions and institutional guidelines for the care and use of animals  X X X X X X X X X X X X X X X X X X  X X  X X X X X X X X X X X X X X X X 36 92.3% Study design Number of animals per group  X X X   X X X X X X X X  X    X X   X X X X X X X  X X X X X X X X 29 74.35% Information on whether the experiment was performed as a blind controlled study     X   X               X    X      X    X   6 15.38% Experimental procedures Treatment X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X 39 100.00% Dosage of treatment X X X X X X X X X X X X  X X X X X X X X X X  X X X X X X X X X X X X X X X 37 94.87% Route of administration X X X X X X X X X X X X X X X X X  X X X X X X X X X X X X X X X X X X X X X 38 97.44% Duration of treatment X X X X  X X   X X X  X X X X X X X X X X X X X X X X X X X X X X X X X X 35 89.74% Time of day for treatment administration  X          X      X X X   X X       X     X    9 23.07% Location used for administration of treatment                            X            1 2.56% Rationale for choice of specific dosage     X  X  X   X X  X     X X  X          X   X   X 12 30.76% Rationale for choice of specific route of administration     X  X  X                           X  X X 6 15.38% Experimental animals Information on animal species X X X X X X X X X X X X X X  X X X X X X X X X X X X X X X X X X X   X X  35 89.74% Strain of the animals  X   X X X X X X X  X   X X X X X X X X X X X   X X X X X X   X X X 29 74.36% Sex of the animals X X X X X X X X X X X X X X X X X X  X X X X X X X X X X X  X X X  X X X X 36 92.30% Animals weight range  X     X     X  X  X X X  X X X  X X  X X X  X X X X   X X X 22 56.41% Age of the animals X  X X X X   X  X  X X X X   X X  X X  X X   X    X X X  X   22 56.41% Description of genetic modification status (knock-out, transgenic, and SPF) X  X X     X X X    X   X X   X X     X     X X  X X X X 18 46.15% Information related to previous procedures performed on the animals  X X X X X X X   X X X X X   X X X X X X X X  X       X   X X X 25 64.10% Housing and husbandry Housing of experimental animals (type of facility, type of cage or housing, material, and number of cage companions)   X    X X   X X X   X   X  X     X X X X        X   14 35.89% Husbandry conditions (breeding programme, light/dark cycle, and temperature of water)  X X X X  X X  X X X X X X X X  X X X X X X X X X X X X X X X X X  X  X 33 84.62% Welfare-related assessments and interventions that were carried out before, during, or after the experiment       X             X X  X              X   5 12.82% Sample size Total number of animals used in each experiment and the number of animals in each experimental group  X X X   X X X X  X X   X     X  X X X X X X X X  X   X  X   22 56.41% Explanation regarding the decision of the number of animals and details of sample size calculation       X                          X       2 5.12% Allocation of animals into experimental groups Full details of how animals were allocated to experimental groups (including randomization or matching)    X   X  X    X     X     X  X   X X   X     X   11 28.20% Order in which the animals in the different experimental groups were treated and assessed X X X X X  X X X  X X      X X X X X X X X X X X  X   X X X  X X X 28 71.79% Experimental outcomes Clear experimental outcomes assessed  X X X X  X X X X X X  X       X X X X X X X X X X X X X X   X X X 28 71.79% Statistical methods Statistical methods used for each analysis X X X X X  X X X X   X X X X X X X X X X X X X X X X X  X X X X X  X X X 34 87.18% Specification of the unit of analysis for each dataset    X X  X X X X  X X X X X X X X X X X X X X X X X X X X X X X X  X X X 33 84.61% Methods used to assess whether the data met the assumptions of the statistical approach X X X X X  X X X X  X X X  X X X X X X X X X X X X X X X X X X X   X X X 34 87.18%  Results Baseline data Description of the animals health status, for each experimental group, before treatment X X X X     X X        X X X X X X X   X  X   X  X   X   18 46.15% Number analyzed Number or animals in each group included in each analysis (absolute numbers)       X X  X      X          X X  X   X X X   X  X 12 30.76% Animals or data not included in the analysis (and explanation for the exclusion)                                  X      1 2.56% Outcomes and estimation Information (mean = standard deviation) X X X X X       X X     X X X X X X X X        X X   X X X 20 51.28% Information on quantification of inflammatory cells (mean = standard deviation)                        X                1 2.56% Adverse events Information on mortality of experimental animal (mean = standard deviation)   X                  X           X        3 7.69% Modifications to the experimental protocols made to reduce adverse events   X                                  X   2 5.12% Discussion Interpretation/scientific implications Interpretation of the results, taking into account the study objectives and hypotheses X X X X  X X X X X X X X X X  X X X X X X X X X X X X X X X X X X   X X X 35 89.74% Comments on the study limitations (sources of bias and imprecision associated with the results)  X X   X X  X X          X             X X   X X X 12 30.76% Generalizability /translation Comments on how the findings are likely to translate to other species or systems (relevance to human biology)   X X X X X X X X  X X  X X X X     X   X X      X X   X X X 22 56.41% Funding List of funding sources and the role of the funder(s) in the study  X X X      X   X X X X X  X X X  X X X X X X X X X X X X  X X X X 28 71.79%  Results 17 28 31 29 24 15 33 26 28 27 23 25 27 23 20 24 21 24 22 27 29 25 32 27 27 27 29 27 28 22 21 25 32 32 16 17 38 26 30


## 2.3. ARRIVE (Bias Analyses)

The detailed reports of experiments are crucial in the review process, so that they can be validated and used as a source of information for further research. However, many studies do not bring relevant or concise information, which leads to the realization of redundant and duplicated experiments [23]. Therefore, guidelines were developed for animal research reports, such as the ARRIVE guideline, based on the CONSORT Statement. The ARRIVE guidance is a list of 20 items that describe the minimum information that all scientific publications reporting research using animals must include, aiming at high quality reports and critical and accurate review of what was performed and found [24]. Thus, based on these fundamentals and the objective of this study, a table displaying the most relevant and applicable items from the ARRIVE was developed for a critical evaluation of the studies included in this review (Table 2). The authors assessed the quality, integrity, and transparency of each publication. Divergent opinions were resolved by consensus.


## 3. Results

### 3.1. Prism

The search conducted in this study found a total of 1,067 papers, out of which 571 were found in PubMed and 495 in Scopus. Out of this total, 449 papers were duplicates; thus 618 studies remained. Then, a title and abstract screening was performed, guided by the eligibility criteria listed above. In this respect, 574 studies were excluded due to inadequate research topic. Among the excluded studies, we can highlight those on the crude extract of the plant (200), studies in languages other than English and Portuguese (63), secondary studies, literature reviews, editorials, comments (60),in vitro studies (54), and studies in which alcohol was administered in the diet (35). Next, 39 studies were selected and their reference lists were screened to identify additional relevant studies missed in the initial search strategy. Thus, all the studies that met the eligibility criteria were included in the review, taking into account the use of fractions and isolates from noncommercial plants in the treatment of type II diabetes in animal models of rats and mice. All search process is shown in Figure 1.


### 3.2. Qualitative Results

With respect to papers reporting treatments with plant fractions (n​​=23), the years of publication ranged from 2001 to 2015. Most studies used rats (60.9%) and mice (39.1%). The sample size varied greatly; some studies used 14 animals and others, 98 animals, while 36.4% of the publications did not report such data. Most studies used male animals, but 2 papers reported the use of both sexes, and 21.7% of the studies did not provide this information. The age of the animals ranged from 3 to 14 weeks and 56.5% of the studies did not report these data. The weight of the animals was not reported in 21.74% of the studies. Only 26.1% of the papers reported if randomization was applied in the experimental groups. Fractions of the extracts were administered orally in 91.29% of the studies and the treatment duration ranged from 7 days to 10 weeks. Regarding the drug used to induce type II diabetes, 60.86% of the studies used streptozotocin; 8.69%, alloxan; and 4.35%, PX-407 (Table 1). China (34.9%) and India (26%) are the countries with the largest number of publications on this subject. Around 47.8% of the studies used a control group. Metformin (30.5%) and glibenclamide (21.7%) were the most commonly used drugs (Figure 2).Figure 2
Summary of the articles describing the main fractions of plants, their species, families, used control groups, and the main countries where researches on this topic have been developed. Data obtained from the qualitative and ethnobotanic analysis. Flowchart fractions.The studies that used plant isolates in the treatment of diabetes (n=16) were carried out from 1998 to 2014. Mice (56.25%), rats (37.50%), and both (6.25%) were the species used in the experiments. The sample size ranged from 12 to 102 animals, and 37.50% of the studies did not report such information. Most studies used male animals (87.50%) and 12.50% used both sexes. The age of the animals ranged from 7 to 26 weeks. The weight of the animals was not reported in 75% of the papers and the strains used were not provided in 31.25% of the studies, while 62.50% of the papers did not report animal randomization. The treatment was administered orally in all analyzed studies (100%). Diabetes was induced with the use of streptozotocin (56.3% of the studies), PEG300 (6.25%), and insulin solution (12.5%) (Table 2). Japan (23.5%) stands out among the countries that have developed studies in the area, followed by India and Taiwan (17.64%) (Figure 3).Figure 3
Summary of the articles describing the main isolates of plants, their species, families, used control groups, and the main countries where a research on this topic has been developed. Data obtained from the qualitative and ethnobotanic analysis. Flowchart isolates.∗The popular name of the plant because the scientific name was not found.The main results for plant fractions and isolates in the treatment of type II diabetes are shown in Figure4. The main findings were (A) reduced blood glucose levels in isolate treatments (25, 27, 30, 31, 32, 33, 34, 35, 36, 37, 39, and 40) and fractions (17, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 56, 57, 58, 59, 60, 61, and 62); (B) normalization of body weight in studies using fractions (17, 43, 44, 49, 50, 52, 55, 57, and 59) while only two studies evaluated this parameter in isolates (27 and 34); (C) normalization of plasma insulin levels during treatment with fractions (17, 45, 47, 48, 49, 50, 51, 53, 55, 58, 60, and 61) and treatment with isolates (25, 26, 27, 28, 29, 30, 34, 36, and 38); (D) reduced total triglycerides and cholesterol in studies using fractions (17, 42, 45, 44, 46, 48, 52, 54, and 59) and isolates (31, 35, 38, and 40); (E) increased glycogen synthesis in studies using fractions (45, 51, and 60) and isolates (27, 29, and 35). Decreased water and food intake has been described in only one isolate (35) and four fractions (41, 51, 54, and 60). The normalization of glycosylated hemoglobin was observed in seven fractions (44, 49, 52, 54, 56, 60, and 61) and one isolate (27) (Figure 4).Figure 4
Main results demonstrating the action of fractions and isolates from plants on the treatment of type II diabetes. Flowchart: isolates and fractions.


### 3.3. ARRIVE (Bias Analysis)

The ARRIVE guidelines were used to assess the quality of the papers under analysis (Table1). After reading and performing critical analyses, the researchers observed that 84.61% of the studies had exact title and concise description. Abstracts describing the purpose, methods, main results, and conclusions were found in 92.30% of the studies. Primary and secondary objectives were clearly stated by 82.05% of the studies, while 92.30% reported in the methodology description that they had obtained permission from the ethics committee for performing the research; on the other hand, experimental information about controlled or blind study was observed in only 15.38%. The animal species were cited in 89.74% of the papers, while weight and sex were described in only 56.41% of the studies. It was observed that 46.15% of the publications reported genetic changes in animals, while lodgment and environmental conditions (light/dark cycle, temperature, and water) were reported in 35.89% and 84.61% of the studies, respectively. Regarding the sample size, 56,41% reported the total number of used animals, but only 5.12% explained the reason for choosing such numbers, and 28.20% of the authors reported the use of randomization. It was observed that 87.17% of the studies specified each statistical analysis method. Only 7.69% of the papers reported the occurrence of animal mortality during the experiment. Among the evaluated discussions, 89.74% interpreted the results taking into account the objectives and hypotheses of the study, current theory, and relevant publications. Only 30.76% commented about the limitations of the studies. Comments on the importance of applying the results to human biology were found in 56.41% of the studies.


## 3.1. Prism

The search conducted in this study found a total of 1,067 papers, out of which 571 were found in PubMed and 495 in Scopus. Out of this total, 449 papers were duplicates; thus 618 studies remained. Then, a title and abstract screening was performed, guided by the eligibility criteria listed above. In this respect, 574 studies were excluded due to inadequate research topic. Among the excluded studies, we can highlight those on the crude extract of the plant (200), studies in languages other than English and Portuguese (63), secondary studies, literature reviews, editorials, comments (60),in vitro studies (54), and studies in which alcohol was administered in the diet (35). Next, 39 studies were selected and their reference lists were screened to identify additional relevant studies missed in the initial search strategy. Thus, all the studies that met the eligibility criteria were included in the review, taking into account the use of fractions and isolates from noncommercial plants in the treatment of type II diabetes in animal models of rats and mice. All search process is shown in Figure 1.


## 3.2. Qualitative Results

With respect to papers reporting treatments with plant fractions (n​​=23), the years of publication ranged from 2001 to 2015. Most studies used rats (60.9%) and mice (39.1%). The sample size varied greatly; some studies used 14 animals and others, 98 animals, while 36.4% of the publications did not report such data. Most studies used male animals, but 2 papers reported the use of both sexes, and 21.7% of the studies did not provide this information. The age of the animals ranged from 3 to 14 weeks and 56.5% of the studies did not report these data. The weight of the animals was not reported in 21.74% of the studies. Only 26.1% of the papers reported if randomization was applied in the experimental groups. Fractions of the extracts were administered orally in 91.29% of the studies and the treatment duration ranged from 7 days to 10 weeks. Regarding the drug used to induce type II diabetes, 60.86% of the studies used streptozotocin; 8.69%, alloxan; and 4.35%, PX-407 (Table 1). China (34.9%) and India (26%) are the countries with the largest number of publications on this subject. Around 47.8% of the studies used a control group. Metformin (30.5%) and glibenclamide (21.7%) were the most commonly used drugs (Figure 2).Figure 2
Summary of the articles describing the main fractions of plants, their species, families, used control groups, and the main countries where researches on this topic have been developed. Data obtained from the qualitative and ethnobotanic analysis. Flowchart fractions.The studies that used plant isolates in the treatment of diabetes (n=16) were carried out from 1998 to 2014. Mice (56.25%), rats (37.50%), and both (6.25%) were the species used in the experiments. The sample size ranged from 12 to 102 animals, and 37.50% of the studies did not report such information. Most studies used male animals (87.50%) and 12.50% used both sexes. The age of the animals ranged from 7 to 26 weeks. The weight of the animals was not reported in 75% of the papers and the strains used were not provided in 31.25% of the studies, while 62.50% of the papers did not report animal randomization. The treatment was administered orally in all analyzed studies (100%). Diabetes was induced with the use of streptozotocin (56.3% of the studies), PEG300 (6.25%), and insulin solution (12.5%) (Table 2). Japan (23.5%) stands out among the countries that have developed studies in the area, followed by India and Taiwan (17.64%) (Figure 3).Figure 3
Summary of the articles describing the main isolates of plants, their species, families, used control groups, and the main countries where a research on this topic has been developed. Data obtained from the qualitative and ethnobotanic analysis. Flowchart isolates.∗The popular name of the plant because the scientific name was not found.The main results for plant fractions and isolates in the treatment of type II diabetes are shown in Figure4. The main findings were (A) reduced blood glucose levels in isolate treatments (25, 27, 30, 31, 32, 33, 34, 35, 36, 37, 39, and 40) and fractions (17, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 56, 57, 58, 59, 60, 61, and 62); (B) normalization of body weight in studies using fractions (17, 43, 44, 49, 50, 52, 55, 57, and 59) while only two studies evaluated this parameter in isolates (27 and 34); (C) normalization of plasma insulin levels during treatment with fractions (17, 45, 47, 48, 49, 50, 51, 53, 55, 58, 60, and 61) and treatment with isolates (25, 26, 27, 28, 29, 30, 34, 36, and 38); (D) reduced total triglycerides and cholesterol in studies using fractions (17, 42, 45, 44, 46, 48, 52, 54, and 59) and isolates (31, 35, 38, and 40); (E) increased glycogen synthesis in studies using fractions (45, 51, and 60) and isolates (27, 29, and 35). Decreased water and food intake has been described in only one isolate (35) and four fractions (41, 51, 54, and 60). The normalization of glycosylated hemoglobin was observed in seven fractions (44, 49, 52, 54, 56, 60, and 61) and one isolate (27) (Figure 4).Figure 4
Main results demonstrating the action of fractions and isolates from plants on the treatment of type II diabetes. Flowchart: isolates and fractions.


## 3.3. ARRIVE (Bias Analysis)

The ARRIVE guidelines were used to assess the quality of the papers under analysis (Table1). After reading and performing critical analyses, the researchers observed that 84.61% of the studies had exact title and concise description. Abstracts describing the purpose, methods, main results, and conclusions were found in 92.30% of the studies. Primary and secondary objectives were clearly stated by 82.05% of the studies, while 92.30% reported in the methodology description that they had obtained permission from the ethics committee for performing the research; on the other hand, experimental information about controlled or blind study was observed in only 15.38%. The animal species were cited in 89.74% of the papers, while weight and sex were described in only 56.41% of the studies. It was observed that 46.15% of the publications reported genetic changes in animals, while lodgment and environmental conditions (light/dark cycle, temperature, and water) were reported in 35.89% and 84.61% of the studies, respectively. Regarding the sample size, 56,41% reported the total number of used animals, but only 5.12% explained the reason for choosing such numbers, and 28.20% of the authors reported the use of randomization. It was observed that 87.17% of the studies specified each statistical analysis method. Only 7.69% of the papers reported the occurrence of animal mortality during the experiment. Among the evaluated discussions, 89.74% interpreted the results taking into account the objectives and hypotheses of the study, current theory, and relevant publications. Only 30.76% commented about the limitations of the studies. Comments on the importance of applying the results to human biology were found in 56.41% of the studies.


## 4. Discussion

This review aimed to describe the main findings in literature on the effects of fractions and isolates obtained from plant extracts on the treatment of type II diabetes in murine models. We believe that the information obtained may help and provide guidance to researchers about the best animal models, drugs, and most used doses in disease induction. Besides, it will guide further research on the most common and important parameters to describe the best results for controlling the metabolic changes caused by the disease. Studies that tested crude plant extracts were not included in this review, due to their wide variability. Studies that obtained fractions and isolates commercially were also excluded. Although species differences prevent the direct extrapolation to clinical applications in humans, the current findings strongly point to the need for a more controlled preclinical research in animals and then in humans, mainly in relation to the doses of fractions and isolates and the most used plant species.The present review showed that isolates or fractions of plants had positive effects on diabetes treatment and reduced various animal blood and tissue parameters that had been changed by the disease. This study also highlights important issues related to the quality of the models and protocols, drugs and doses used in the study for inducing the disease, the most commonly used administration routes, and main tests used for disorder confirmation. Systematic review studies are focused on the assessment of the quality of the reviewed studies, using acknowledged scales and protocols. Although these scales have not been formally developed for experimental model studies, the assessment of the quality of the reviewed studies considered the items normally included in scales for randomized clinical studies. Therefore, we used the ARRIVE platform for work quality analysis and observed that most studies did not provide many details about the materials and methods used, which prevents the replication of some studies. There were no reports of the number of animals used, age, weight, and even the presence of randomization to reduce bias in the selection of the animals and assessment of the results in many studies. These findings corroborate the need for guidelines to describe the required information for all scientific publications that use animals as experimental models [24]. In this study, out of the 618 articles analyzed, 39 were selected according to the eligibility criteria and the proposed objective. The PRISMA recommendations were used to guide the development of this systematic review and improve the visualization of the steps of an effective search [22].Most animals studied were male, since males suffer less hormonal fluctuation and hence less change in behavior compared to females [62]. The number of studies with rats (n=20) and mice (n=19) was very close. However, is it possible to detect the increasing use of mice in preclinical experiments, due to the genetic similarities between this species and humans. According to Machado and Zatti [63], about 99% of human genes have been mapped in mouse, which allows the association between them. Moreover, it must be taken into account that the small size of these animals reduces the costs of the experiment and makes it easy to handle and perform a great number of procedures. There was wide variation in the age of the animals. The youngest animals were 3 weeks old and the oldest, 26 weeks. In addition, many studies did not provide such information (n=18). The weight of the animals ranged on average from 25.5 g in mice to 61 g in rats. The authors attribute this great variability to the discrepancies in the age of the animals. Besides, the variable weight was not reported in 38.4% of the studies. The number of studies that did not describe variables such as age and weight is worrying, since these characteristics are important for further replication of the studies and elaboration of extensive reports on the procedures adopted [64].Streptozotocin, either combined or not with another drug, was the main drug selected for type II diabetes induction in animals. Streptozotocin is a large spectrum antibiotic, used as a diabetogenic agent in experimental animals [65]. This action is mediated by the destruction of beta cells in the pancreas, which leads to insulin deficiency and also occurs in human type II diabetes in relation to metabolic characteristics [66]. Wide variation was observed in the streptozotocin dose used to induce diabetes, from 20 mg/kg to 137 mg/kg. The analysis of works with drug-induced diabetes mainly requires the establishment of the most appropriate dose and the correct administration time, since these variables may reduce the time and costs of the experiment. In this review, after the analysis of the work, no consensus was found for the best dose and timing for drug application. Metformin was the main drug selected for the control group, due to its relevant clinical use, favorable toxicity profile, and safety. Besides, it is well tolerated during treatment [67].Although many drugs have been used for diabetes treatment, some of them are expensive and inefficient and cause severe side effects. Thus, there is a growing interest from researchers and pharmaceutical companies in the development of alternative drugs, such as medicinal plants, for diabetes treatment [68–70]. However, further studies should be conducted, since some plants associated with diabetes mellitus treatment are considered toxic and may cause various tissue lesions [71]. The present study reports several plant species that have been used to obtain both fractions and isolates. Most studies were carried out in China, India, and Japan. These countries have shown great interest in the development of drugs from plant extracts, due to their great flora diversity. Japan has always had an interest in the development of new technologies. Phytotherapy is promising for health care in many ways. The analysis of the results of the studies revealed that most authors reported decreased blood glucose in treated diabetic animals (n=33) and normalized plasma insulin levels (n=21). Postprandial hyperglycemia is a common pathogenesis of type II diabetes induced by insulin resistance, as well as the partial destruction of pancreas β cells [72–75]. The effective control of blood glucose and insulin level is a key step in preventing or reversing diabetic complications and improving the life quality of patients [76]. Hyperlipidemia is another complication caused by diabetes, characterized by high cholesterol and triglyceride levels and lipoprotein composition changes [77]. These data were analyzed in this study, due to their relevance. Thirteen studies reported decreased total cholesterol and triglyceride levels in animals treated with plant derived medicine.Besides, polydipsia, polyphagia, and changes in weight (weight loss or gain) are common occurrences in patients with diabetes [78]. Weight loss is usually observed when the disease is acquired and can be associated with dehydration and catabolism of fat tissue or protein degradation and consequent muscle mass loss. According to this review, many studies reported increased animal water and food intake, as well as weight normalization. These data can be justified by the increased glucose and insulin uptake and decreased secretion of blood glucose, which indicate improved animal glycemic control [49]. These improved results in the body weight of diabetic animals are consistent and were reported by some studies using medicinal plants with potential antidiabetic effects [79, 80]. The increased glycogen synthesis was also analyzed, since the liver metabolism of this substance regulates glucose blood level [50]. In addition, some studies reported the normalization of glycated hemoglobin levels, which is important to assess diabetes control levels, since its dosage directly reflects the average blood glucose levels, from two to three months prior to the collection of the biological material [81].


## 5. Conclusion

The results of this study demonstrate that plant fractions and isolates improve the main physiological and morphological changes caused by type II diabetes and decrease food and water intake, total cholesterol, triglycerides, and glucose, thus normalizing body weight and blood insulin levels. However, serious methodological problems were found in many studies, including errors in the details of the procedures performed, which prevents the understanding of some studies and hinders the use of the data found in animals for studies on human clinical condition. Therefore, the improvements in research reports on preclinical studies require a collective effort from authors, journal editors, reviewers, and funding agencies to ensure that the papers will allow other researchers to reproduce the study.



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