# Effect of Extraction Methods on Antioxidant Activity of Papery Skin Extracts and Fractions of Maja Cipanas Onion (Allium cepa L. var. ascalonicum)

Authors: Nyi M. Saptarini; Yulia Wardati
Journal: The Scientific World Journal
 (2020)

---

## Abstract

Extraction can be carried out at ambient temperature or high temperature to accelerate the extraction process of secondary metabolites from simplicia. This study aimed to determine the effectiveness of extraction methods on antioxidant activity of secondary metabolites of papery skin extracts and fractions of Maja Cipanas onion (Allium cepa L. var. ascalonicum). Extraction methods were maceration, percolation, reflux, and Soxhlet method, and then, concentrated extracts were fractionated by liquid-liquid extraction based on the polarity of secondary metabolites. Antioxidant activity was determined by the DPPH (2,2-diphenyl-1-picrylhydrazyl) method. The phytochemical screening showed that onion papery skin contained alkaloids, polyphenols, flavonoids, and tannins. The IC50 value of the extract, ethyl acetate fraction, and water fraction of the four extraction methods in the concentration range 25–400  μg/mL were in the range of 55.62–107.08, 31.31–84.06, and 126.05–139.82  μg/mL, respectively, while the IAA value was in the ranges of 0.25–0.49, 0.32–0.86, and 0.19–0.21, respectively. Variation in IC50 and IAA values indicate that the extraction method affects antioxidant activity, due to extracted secondary metabolites from simplicia. The highest antioxidant activity was an ethyl acetate fraction by the reflux method, while the lowest was water fraction by the percolation method.


---

## Body

## 1. Introduction

The production of onion (Allium cepa L., Liliaceae) in Indonesia was 1,470,155 tons in 2017 and increased to 1,503,436 tons in 2018, about 2.26% [1]. Onion production is fluctuating because of the imbalance of production between in-season and off-season harvest [2]. This is due to the high intensity of pest and disease if planted in out of season [3, 4].The used part of the onion is the bulbs, while the papery skin is thrown away. The onion papery skin has not been utilized, although it contains anthocyanins [5]. Previous studies showed that Maja Cipanas onion had higher anthocyanin content than Bima Brebes onion [6] and the production of Maja Cipanas onion (10.9 tons/ha) was higher than Bima Brebes onion (9.9 tons/ha) in 2015 [1]. Anthocyanins are flavonoid derivate, which have antioxidant activity [7]. This study aimed to determine the effectiveness of extraction methods on antioxidant activity of secondary metabolites of papery skin extracts and fractions of Maja Cipanas onion (Allium cepa L. var. ascalonicum).


## 2. Materials and Methods

### 2.1. Materials

Maja Cipanas Onion (Allium cepa L. var. ascalonicum) bulbs were collected from the Gede Bage market, West Java Province, Indonesia in July 2016. Bulbs were identified by the School of Biological Sciences and Technology, Bandung Institute of Technology, Indonesia, with No. 912/II.CO2.2/PL/2016. All chemical reagents are of analytical grade (Merck, Germany), i.e., ethanol, hydrochloride acid, sodium hydroxide, n-buthanol, acetic acid, potassium chloride, potassium chloride, and sodium acetate.


### 2.2. Water Determination

Onion papery skin was peeled, washed, and dried at 40  C. Two grams of onion papery skin were dried on 105°C at an atmospheric pressure for 5  h and then weighed. Drying and weighing continued, every 1  h, until a constant weight [8].


### 2.3. Extraction

Each of 30  g onion papery skin was extracted with 300  mL of a mixture of 70% ethanol and 2 N hydrochloride acid, with a pH of 1.0 in a macerator, percolator, and reflux and Soxhlet apparatus. The extraction was repeated for three cycles with solvent replacement. Each cycle was 24  h for maceration, 8  h for percolation, and 2  h for reflux and Soxhlet method [6]. All extracts were collected and concentrated with a rotary evaporator at 40°C, and then, the yield was calculated.


### 2.4. Fractionation

The concentrated extract was dissolved in 40  mL of 60°C water and then put into a separating funnel, and 20  mL ofn-hexane was added. The separating funnel was closed and shaken and then allowed to separate. The two fractions were separated into two different containers. The water fraction was put back into the separating funnel, and then, another 20  mL of n-hexane was added and carried out as above. Repeat the fractionation until uncolored n-hexane fraction is obtained. The n-hexane fraction is then held in the same container. The water fraction was placed into a separating funnel and fractioned using ethyl acetate in the same way as fractioned with n-hexane. Ethyl acetate and water fraction were placed in different containers and then concentrated, and the yield was calculated [9].


### 2.5. Phytochemical Screening

Fransworth method was conducted for phytochemical screening to simplicia, extracts, and fraction [10].


### 2.6. Antioxidant Activity Assay

About 40  μg/mL DPPH solution was added to 96% ethanol and allowed to stand for 20  min in a dark place. The absorbance was measured in 400–700  nm. Ascorbic acid standard, extract, and fraction were dissolved in 96% ethanol and diluted into five concentrations. Each sample (1  mL) was added to 2  ml of DPPH solution and incubated for 20  min. Absorbance was measured at maximum wavelength, and % inhibition was calculated using equation (1). The IC50 value was calculated from linear regression between % inhibition and concentration. The antioxidant activity category based on the IC50 value is very strong when IC50 < 50  μg/mL, strong when IC50 is between 50 and 100  μg/mL, moderate when IC50 is between 100 and 150  μg/mL, and low when IC50 > 150  μg/mL [11]. The antioxidant activity index was calculated using equation (2). The antioxidant activity category based on the AAI value is low when AAI <0.5, moderate when AAI is between 0.5 and 1.0, strong when AAI is between 1.0 and 2.0, and very strong when AAI >2.0 [12]:(1)%inhibition=A DPPH−A sampleA DPPH×100%,(2)antioxidant activity indexAAI=final DPPH concentrationμg/mLIC50μg/mL.


### 2.7. Statistical Analysis

Results are presented as the mean ± standard deviation (SD). One-way ANOVA followed by Student’st-test was used to analyze the data comparisons between groups. Values were considered statistically significant at p<0.05.


## 2.1. Materials

Maja Cipanas Onion (Allium cepa L. var. ascalonicum) bulbs were collected from the Gede Bage market, West Java Province, Indonesia in July 2016. Bulbs were identified by the School of Biological Sciences and Technology, Bandung Institute of Technology, Indonesia, with No. 912/II.CO2.2/PL/2016. All chemical reagents are of analytical grade (Merck, Germany), i.e., ethanol, hydrochloride acid, sodium hydroxide, n-buthanol, acetic acid, potassium chloride, potassium chloride, and sodium acetate.


## 2.2. Water Determination

Onion papery skin was peeled, washed, and dried at 40  C. Two grams of onion papery skin were dried on 105°C at an atmospheric pressure for 5  h and then weighed. Drying and weighing continued, every 1  h, until a constant weight [8].


## 2.3. Extraction

Each of 30  g onion papery skin was extracted with 300  mL of a mixture of 70% ethanol and 2 N hydrochloride acid, with a pH of 1.0 in a macerator, percolator, and reflux and Soxhlet apparatus. The extraction was repeated for three cycles with solvent replacement. Each cycle was 24  h for maceration, 8  h for percolation, and 2  h for reflux and Soxhlet method [6]. All extracts were collected and concentrated with a rotary evaporator at 40°C, and then, the yield was calculated.


## 2.4. Fractionation

The concentrated extract was dissolved in 40  mL of 60°C water and then put into a separating funnel, and 20  mL ofn-hexane was added. The separating funnel was closed and shaken and then allowed to separate. The two fractions were separated into two different containers. The water fraction was put back into the separating funnel, and then, another 20  mL of n-hexane was added and carried out as above. Repeat the fractionation until uncolored n-hexane fraction is obtained. The n-hexane fraction is then held in the same container. The water fraction was placed into a separating funnel and fractioned using ethyl acetate in the same way as fractioned with n-hexane. Ethyl acetate and water fraction were placed in different containers and then concentrated, and the yield was calculated [9].


## 2.5. Phytochemical Screening

Fransworth method was conducted for phytochemical screening to simplicia, extracts, and fraction [10].


## 2.6. Antioxidant Activity Assay

About 40  μg/mL DPPH solution was added to 96% ethanol and allowed to stand for 20  min in a dark place. The absorbance was measured in 400–700  nm. Ascorbic acid standard, extract, and fraction were dissolved in 96% ethanol and diluted into five concentrations. Each sample (1  mL) was added to 2  ml of DPPH solution and incubated for 20  min. Absorbance was measured at maximum wavelength, and % inhibition was calculated using equation (1). The IC50 value was calculated from linear regression between % inhibition and concentration. The antioxidant activity category based on the IC50 value is very strong when IC50 < 50  μg/mL, strong when IC50 is between 50 and 100  μg/mL, moderate when IC50 is between 100 and 150  μg/mL, and low when IC50 > 150  μg/mL [11]. The antioxidant activity index was calculated using equation (2). The antioxidant activity category based on the AAI value is low when AAI <0.5, moderate when AAI is between 0.5 and 1.0, strong when AAI is between 1.0 and 2.0, and very strong when AAI >2.0 [12]:(1)%inhibition=A DPPH−A sampleA DPPH×100%,(2)antioxidant activity indexAAI=final DPPH concentrationμg/mLIC50μg/mL.


## 2.7. Statistical Analysis

Results are presented as the mean ± standard deviation (SD). One-way ANOVA followed by Student’st-test was used to analyze the data comparisons between groups. Values were considered statistically significant at p<0.05.


## 3. Results and Discussion

### 3.1. Water Determination

Maja Cipanas onion is resistant to tuber blight due toBotrytis allii and potential for growing in Indonesia. A total of 5  kg of Maja Cipanas onion was produced from 36  g of dry papery skin onion with 0.72% of yield. The simplicia moisture content was 4.2 ± 0.5%, which met the requirement, i.e., less than 8%. High water content causes secondary metabolite degradation due to microorganism growth [13]. Maja Cipanas onion has pinky red papery skin [1], which suggested anthocyanins [14]. The best solvent for anthocyanin extraction is acetic acid or hydrochloric acid-contained solvent [15]. Our previous study showed that the mixture of 70% ethanol and HCl with pH 1.0 was best solvent for anthocyanin extraction [6]. All extracts of papery skin Maja Cipanas onion were red, due to an oxonium form of anthocyanin [14], with various yields and intensity because of the different extraction methods (Table 1).Table 1
The yield of extraction.
Method
Simplicia (g)
Extract (g)
Yield (%)
Color
Maceration
30
9.58
31.92
Red
Percolation
30
11.88
39.6
Dark red
Reflux method
30
9.70
32.34
Red
Soxhlet method
30
8.81
34.05
Light redThe concentrated extracts were produced from cold extraction (maceration and percolation) and hot extraction (reflux and Soxhlet extraction). The efficiency of maceration and reflux extraction, i.e., the soaking method, depends on the effective diffusion and solubility of secondary metabolites [16]. The yield of maceration was lower than reflux extraction, because heating in reflux extraction increases diffusion [17]. The efficiency of percolation and Soxhlet extraction, i.e., the flowing solvent method, depends on repeated cycle extraction with a fresh solvent until all the solutes are dissolved in simplicia [18]. The yield of Soxhlet extraction was lower than percolation, due to anthocyanin instability to temperature [14]. Percolation was the extraction method with the highest yield (Table 1). This result showed that secondary metabolites can be completely extracted using flowing acidified solvents at ambient temperature.The yield of water fractions was higher than of the ethyl acetate fractions (Table2), due to the polarity of acidified ethanol as the extraction solvent, in which polar secondary metabolites extracted higher than nonpolar ones. The n-hexane fractions were not obtained because n-hexane is a nonpolar solvent, while the extraction solvent is polar acidified ethanol. It is suggested that no nonpolar secondary metabolites were extracted.Table 2
The yield of fractionation.
Method
Water fraction
Ethyl acetate fraction
Maceration
67.88
23.69
Percolation
66.67
23.08
Reflux method
66.82
22.47
Soxhlet method
57.27
26.36Alkaloids in sample solution were reacted with Dragendorff's reagent to produce an orange to orange-red precipitate, which accorded with the literature [19]. Polyphenols in sample solution were reacted with iron III chloride (FeCl3) to produce the green solution. These results were accorded with the literature, in which complexes of polyphenols-FeCl3 are blue, green, red, or purple color [20]. Tannins in sample solution were reacted with a gelatin solution containing sodium chloride to produce white precipitate, which accorded with the literature [20]. Flavonoids in sample solution were reacted with magnesium powder and concentrated hydrochloric acid to produce pink to red color. These results were accorded with the literature, in which flavonoids produce pink or red color with this reagent [21]. The phytochemical screening showed that simplicia, extracts, ethyl acetate fractions, and water fractions from the maceration, percolation, reflux, and Soxhlet method contain alkaloids, polyphenols, flavonoids, and tannins. These results show that all extraction methods can dissolve the same secondary metabolites in different quantities, which can be observed from the extract color (Table 1).


### 3.2. Antioxidant Activity Assay

DPPH free radicals are organic compounds with unstable nitrogen, due to unpaired electrons [22]. DPPH solution is dark purple with a maximum wavelength of 518  nm (Figure 1). The oxidant compounds give the hydrogen atom to the DPPH free radical, which reduced to DPPH-H (1,1-diphenyl-2-picrylhydrazine) [22]. The absorbances of extract and fraction of Maja Cipanas onion were able to donate its hydrogen atoms in sufficient quantities to scavenging the DPPH free radicals (Table 3).Figure 1
Maximum wavelength of DPPH solution. The purple DPPH solution has a maximum wavelength at 518  nm.Table 3
Antioxidant activity of sample to DPPH solution (n = 3).
Extraction method
Sample (n = 3)
Concentration (μg/mL)
% Scavenging
Linear equation
IC50 value (μg/mL)
AAI
None
Ascorbic acid
2
45.51 ± 0.03
y = 6.52x + 33.00 R2 = 0.998
2.60
10.38
4
59.12 ± 0.08
6
72.90 ± 0.04
8
85.74 ± 0.06
10
97.45 ± 0.07
Maceration
Extract
25
43.86 ± 0.04
y = 0.083x + 42.86R2 = 0.9966
86.28
0.31
50
47.86 ± 0.05
100
51.17 ± 0.03
200
59.86 ± 0.08
400
75.72 ± 0.06
Ethyl acetate fraction
25
48.28 ± 0.06
y = 0.083x + 47.11 R2 = 0.9975
34.92
0.77
50
52.00 ± 0.03
100
55.31 ± 0.04
200
64.00 ± 0.05
400
79.87 ± 0.07
Water fraction
25
37.10 ± 0.08
y = 0.106x + 36.04  R2 = 0.9919
131.69
0.21
50
40.97 ± 0.04
100
47.86 ± 0.06
200
59.03 ± 0.03
400
77.38 ± 0.05
Percolation
Extract
25
47.12 ± 0.05
y = 0.067x + 46.12 R2 = 0.9907
55.62
0.49
50
50.85 ± 0.03
100
53.05 ± 0.06
200
58.47 ± 0.04
400
77.73 ± 0.07
Ethyl acetate fraction
25
49.15 ± 0.04
y = 0.069x + 47.08 R2 = 0.992
41.91
0.64
50
50.85 ± 0.05
100
54.41 ± 0.08
200
59.32 ± 0.03
400
75.59 ± 0.06
Water fraction
25
34.07 ± 0.05
y = 0.129x + 31.95 R2 = 0.993
139.82
0.19
50
37.46 ± 0.04
100
45.76 ± 0.08
200
60.17 ± 0.03
400
82.37 ± 0.07
Reflux method
Extract
25
46.63 ± 0.03
y = 0.064x + 45.02 R2 = 0.9934
78.07
0.35
50
49.19 ± 0.05
100
50.93 ± 0.04
200
56.74 ± 0.07
400
71.05 ± 0.08
Ethyl acetate fraction
25
73.84 ± 0.05
y = 0.066x + 47.95 R2 = 0.9923
31.31
0.86
50
61.98 ± 0.03
100
53.84 ± 0.04
200
52.21 ± 0.08
400
48.72 ± 0.06
Water fraction
25
38.84 ± 0.08
y = 0.089x + 37.94 R2 = 0.9919
134.88
0.20
50
42.09 ± 0.07
100
47.91 ± 0.04
200
57.33 ± 0.05
400
72.79 ± 0.03
Soxhlet method
Extract
25
38.62 ± 0.09
y = 0.116x + 37.64 R2 = 0.9929
107.08
0.25
50
45.75 ± 0.06
100
49.31 ± 0.03
200
60.57 ± 0.04
400
84.25 ± 0.05
Ethyl acetate fraction
25
45.50 ± 0.09
y = 0.081x + 43.40  R2 = 0.9955
84.06
0.32
50
48.51 ± 0.05
100
51.26 ± 0.07
200
58.51 ± 0.04
400
76.44 ± 0.03
50
48.51 ± 0.05
Water fraction
25
40.69 ± 0.07
y = 0.086x + 39.13 R2 = 0.9948
126.05
0.21
50
42.76 ± 0.04
100
48.51 ± 0.03
200
57.82 ± 0.05
400
73.10 ± 0.06Three of the extracts were strong antioxidant (55.62–86.28μg/mL), except the Soxhlet extract was moderate antioxidant (107.08  μg/mL). All ethyl acetate fractions were strong antioxidant (31.31–84.06  μg/mL) and all water fractions were moderate antioxidant (126.05–139.82  μg/mL). The AAI value was calculated with equation (2) [12], and the final DPPH concentration was 30.75  μg/mL, resulting in a constant for each extract and fraction. In this work, we considered that all extracts and water fractions from all extraction methods showed low antioxidant (0.19–0.49), while all ethyl acetate fractions, except for the reflux method, were moderate antioxidant (0.64–0.86). The difference in the strength of antioxidant activity was due to differences in the content and quantity of extracting secondary metabolites in extracts and fractions. Antioxidant activity calculated by AAI is lower than the IC50 value, but the AAI value is more accurate, due to independent on DPPH and sample concentrations [12].Anthocyanins have antioxidant activity [7]. In this study, total anthocyanin contents were not compared with antioxidant activity (Table 4). The total anthocyanin content of the extract was significantly different in various extraction methods (p=3.62×10−6). This showed that the antioxidant activity was given not only by anthocyanins, but mixed secondary metabolites in papery skin onion, i.e., alkaloids, polyphenols, flavonoids, and tannins. Alkaloids and polyphenols, including flavonoids and tannins, are the major antioxidants with antioxidant activities in natural products [23, 24].Table 4
The IC50 value and anthocyanin content (n = 3).
Extraction method
IC50 value (μg/mL)
Total anthocyanin content [6]
Extract
Ethyl acetate fraction
Water fraction
Maceration
86.28
34.92
131.69
1.181 ± 0.008
Percolation
55.62
41.91
139.82
0.597 ± 0.015
Reflux method
78.07
31.31
134.88
1.449 ± 0.013
Soxhlet method
107.08
84.06
126.05
0.342 ± 0.022The antioxidant activity of extracts and fractions was significantly different (p=9.77×10−3). The best antioxidant activity was extract from percolation (p=3.55×10−9), ethyl acetate fraction from the reflux method (p=4.78×10−10), and the water fraction from the Soxhlet method (p=2.41×10−7). This showed that the extraction method affects the extracted secondary metabolites from simplification, thus affecting the extracted secondary metabolites into the ethyl acetate and water fractions.


## 3.1. Water Determination

Maja Cipanas onion is resistant to tuber blight due toBotrytis allii and potential for growing in Indonesia. A total of 5  kg of Maja Cipanas onion was produced from 36  g of dry papery skin onion with 0.72% of yield. The simplicia moisture content was 4.2 ± 0.5%, which met the requirement, i.e., less than 8%. High water content causes secondary metabolite degradation due to microorganism growth [13]. Maja Cipanas onion has pinky red papery skin [1], which suggested anthocyanins [14]. The best solvent for anthocyanin extraction is acetic acid or hydrochloric acid-contained solvent [15]. Our previous study showed that the mixture of 70% ethanol and HCl with pH 1.0 was best solvent for anthocyanin extraction [6]. All extracts of papery skin Maja Cipanas onion were red, due to an oxonium form of anthocyanin [14], with various yields and intensity because of the different extraction methods (Table 1).Table 1
The yield of extraction.
Method
Simplicia (g)
Extract (g)
Yield (%)
Color
Maceration
30
9.58
31.92
Red
Percolation
30
11.88
39.6
Dark red
Reflux method
30
9.70
32.34
Red
Soxhlet method
30
8.81
34.05
Light redThe concentrated extracts were produced from cold extraction (maceration and percolation) and hot extraction (reflux and Soxhlet extraction). The efficiency of maceration and reflux extraction, i.e., the soaking method, depends on the effective diffusion and solubility of secondary metabolites [16]. The yield of maceration was lower than reflux extraction, because heating in reflux extraction increases diffusion [17]. The efficiency of percolation and Soxhlet extraction, i.e., the flowing solvent method, depends on repeated cycle extraction with a fresh solvent until all the solutes are dissolved in simplicia [18]. The yield of Soxhlet extraction was lower than percolation, due to anthocyanin instability to temperature [14]. Percolation was the extraction method with the highest yield (Table 1). This result showed that secondary metabolites can be completely extracted using flowing acidified solvents at ambient temperature.The yield of water fractions was higher than of the ethyl acetate fractions (Table2), due to the polarity of acidified ethanol as the extraction solvent, in which polar secondary metabolites extracted higher than nonpolar ones. The n-hexane fractions were not obtained because n-hexane is a nonpolar solvent, while the extraction solvent is polar acidified ethanol. It is suggested that no nonpolar secondary metabolites were extracted.Table 2
The yield of fractionation.
Method
Water fraction
Ethyl acetate fraction
Maceration
67.88
23.69
Percolation
66.67
23.08
Reflux method
66.82
22.47
Soxhlet method
57.27
26.36Alkaloids in sample solution were reacted with Dragendorff's reagent to produce an orange to orange-red precipitate, which accorded with the literature [19]. Polyphenols in sample solution were reacted with iron III chloride (FeCl3) to produce the green solution. These results were accorded with the literature, in which complexes of polyphenols-FeCl3 are blue, green, red, or purple color [20]. Tannins in sample solution were reacted with a gelatin solution containing sodium chloride to produce white precipitate, which accorded with the literature [20]. Flavonoids in sample solution were reacted with magnesium powder and concentrated hydrochloric acid to produce pink to red color. These results were accorded with the literature, in which flavonoids produce pink or red color with this reagent [21]. The phytochemical screening showed that simplicia, extracts, ethyl acetate fractions, and water fractions from the maceration, percolation, reflux, and Soxhlet method contain alkaloids, polyphenols, flavonoids, and tannins. These results show that all extraction methods can dissolve the same secondary metabolites in different quantities, which can be observed from the extract color (Table 1).


## 3.2. Antioxidant Activity Assay

DPPH free radicals are organic compounds with unstable nitrogen, due to unpaired electrons [22]. DPPH solution is dark purple with a maximum wavelength of 518  nm (Figure 1). The oxidant compounds give the hydrogen atom to the DPPH free radical, which reduced to DPPH-H (1,1-diphenyl-2-picrylhydrazine) [22]. The absorbances of extract and fraction of Maja Cipanas onion were able to donate its hydrogen atoms in sufficient quantities to scavenging the DPPH free radicals (Table 3).Figure 1
Maximum wavelength of DPPH solution. The purple DPPH solution has a maximum wavelength at 518  nm.Table 3
Antioxidant activity of sample to DPPH solution (n = 3).
Extraction method
Sample (n = 3)
Concentration (μg/mL)
% Scavenging
Linear equation
IC50 value (μg/mL)
AAI
None
Ascorbic acid
2
45.51 ± 0.03
y = 6.52x + 33.00 R2 = 0.998
2.60
10.38
4
59.12 ± 0.08
6
72.90 ± 0.04
8
85.74 ± 0.06
10
97.45 ± 0.07
Maceration
Extract
25
43.86 ± 0.04
y = 0.083x + 42.86R2 = 0.9966
86.28
0.31
50
47.86 ± 0.05
100
51.17 ± 0.03
200
59.86 ± 0.08
400
75.72 ± 0.06
Ethyl acetate fraction
25
48.28 ± 0.06
y = 0.083x + 47.11 R2 = 0.9975
34.92
0.77
50
52.00 ± 0.03
100
55.31 ± 0.04
200
64.00 ± 0.05
400
79.87 ± 0.07
Water fraction
25
37.10 ± 0.08
y = 0.106x + 36.04  R2 = 0.9919
131.69
0.21
50
40.97 ± 0.04
100
47.86 ± 0.06
200
59.03 ± 0.03
400
77.38 ± 0.05
Percolation
Extract
25
47.12 ± 0.05
y = 0.067x + 46.12 R2 = 0.9907
55.62
0.49
50
50.85 ± 0.03
100
53.05 ± 0.06
200
58.47 ± 0.04
400
77.73 ± 0.07
Ethyl acetate fraction
25
49.15 ± 0.04
y = 0.069x + 47.08 R2 = 0.992
41.91
0.64
50
50.85 ± 0.05
100
54.41 ± 0.08
200
59.32 ± 0.03
400
75.59 ± 0.06
Water fraction
25
34.07 ± 0.05
y = 0.129x + 31.95 R2 = 0.993
139.82
0.19
50
37.46 ± 0.04
100
45.76 ± 0.08
200
60.17 ± 0.03
400
82.37 ± 0.07
Reflux method
Extract
25
46.63 ± 0.03
y = 0.064x + 45.02 R2 = 0.9934
78.07
0.35
50
49.19 ± 0.05
100
50.93 ± 0.04
200
56.74 ± 0.07
400
71.05 ± 0.08
Ethyl acetate fraction
25
73.84 ± 0.05
y = 0.066x + 47.95 R2 = 0.9923
31.31
0.86
50
61.98 ± 0.03
100
53.84 ± 0.04
200
52.21 ± 0.08
400
48.72 ± 0.06
Water fraction
25
38.84 ± 0.08
y = 0.089x + 37.94 R2 = 0.9919
134.88
0.20
50
42.09 ± 0.07
100
47.91 ± 0.04
200
57.33 ± 0.05
400
72.79 ± 0.03
Soxhlet method
Extract
25
38.62 ± 0.09
y = 0.116x + 37.64 R2 = 0.9929
107.08
0.25
50
45.75 ± 0.06
100
49.31 ± 0.03
200
60.57 ± 0.04
400
84.25 ± 0.05
Ethyl acetate fraction
25
45.50 ± 0.09
y = 0.081x + 43.40  R2 = 0.9955
84.06
0.32
50
48.51 ± 0.05
100
51.26 ± 0.07
200
58.51 ± 0.04
400
76.44 ± 0.03
50
48.51 ± 0.05
Water fraction
25
40.69 ± 0.07
y = 0.086x + 39.13 R2 = 0.9948
126.05
0.21
50
42.76 ± 0.04
100
48.51 ± 0.03
200
57.82 ± 0.05
400
73.10 ± 0.06Three of the extracts were strong antioxidant (55.62–86.28μg/mL), except the Soxhlet extract was moderate antioxidant (107.08  μg/mL). All ethyl acetate fractions were strong antioxidant (31.31–84.06  μg/mL) and all water fractions were moderate antioxidant (126.05–139.82  μg/mL). The AAI value was calculated with equation (2) [12], and the final DPPH concentration was 30.75  μg/mL, resulting in a constant for each extract and fraction. In this work, we considered that all extracts and water fractions from all extraction methods showed low antioxidant (0.19–0.49), while all ethyl acetate fractions, except for the reflux method, were moderate antioxidant (0.64–0.86). The difference in the strength of antioxidant activity was due to differences in the content and quantity of extracting secondary metabolites in extracts and fractions. Antioxidant activity calculated by AAI is lower than the IC50 value, but the AAI value is more accurate, due to independent on DPPH and sample concentrations [12].Anthocyanins have antioxidant activity [7]. In this study, total anthocyanin contents were not compared with antioxidant activity (Table 4). The total anthocyanin content of the extract was significantly different in various extraction methods (p=3.62×10−6). This showed that the antioxidant activity was given not only by anthocyanins, but mixed secondary metabolites in papery skin onion, i.e., alkaloids, polyphenols, flavonoids, and tannins. Alkaloids and polyphenols, including flavonoids and tannins, are the major antioxidants with antioxidant activities in natural products [23, 24].Table 4
The IC50 value and anthocyanin content (n = 3).
Extraction method
IC50 value (μg/mL)
Total anthocyanin content [6]
Extract
Ethyl acetate fraction
Water fraction
Maceration
86.28
34.92
131.69
1.181 ± 0.008
Percolation
55.62
41.91
139.82
0.597 ± 0.015
Reflux method
78.07
31.31
134.88
1.449 ± 0.013
Soxhlet method
107.08
84.06
126.05
0.342 ± 0.022The antioxidant activity of extracts and fractions was significantly different (p=9.77×10−3). The best antioxidant activity was extract from percolation (p=3.55×10−9), ethyl acetate fraction from the reflux method (p=4.78×10−10), and the water fraction from the Soxhlet method (p=2.41×10−7). This showed that the extraction method affects the extracted secondary metabolites from simplification, thus affecting the extracted secondary metabolites into the ethyl acetate and water fractions.


## 4. Conclusions

The extraction method affects extracted secondary metabolites, due to antioxidant activity.



---

*Source: 3280534-2020-04-16.xml*