# Prevalence of Genotypes and Subtypes ofGardnerella vaginalis in South African Pregnant Women

Authors: Kayla Pillay; Silondiwe Nzimande; Meleshni Naicker; Veron Ramsuran; Partson Tinarwo; Nathlee Abbai
Journal: Infectious Diseases in Obstetrics and Gynecology
 (2020)

---

## Abstract

Background. Gardnerella vaginalis, a microorganism highly linked to bacterial vaginosis (BV), is understudied in terms of genotypic heterogeneity in South African populations. This study investigated the prevalence of G. vaginalis genotypes in BV-positive, BV-intermediate, and BV-negative South African pregnant women. Methods. The study population included n=354 pregnant women recruited from a public hospital in Durban, South Africa. The women provided self-collected vaginal swabs for BV diagnosis by Nugent scoring. For the genotyping assays, the 16S rRNA and sialidase A genes from BV-negative, BV-intermediate, and BV-positive samples were amplified with G. vaginalis-specific primers. The16S rRNA amplicon was digested with TaqI to generate genotyping profiles, and subtypes were determined by correlating BamHI and HindIII digestion profiles. Phylogenetic analysis was performed on the 16S rRNA and sialidase A sequences. The data analysis was performed with R Statistical Computing software, version 3.6.2. Results. Two different genotypes, GT1 and GT2, were detected. The most prevalent genotype was GT1. Four subtypes (1, 2B, 2AB, and 2C) were shown to be present. The most prevalent subtype was 2B, followed by subtypes 1, 2C, and 2AB. The phylogenetic analysis of the 16S rRNA showed the presence of 5 clusters. The tree displayed clusters which contained sequences from the same BV group with different genotypes and subtypes. Clusters with sequences from across the BV groups carrying the same genotype and subtype were present. Diversity of the sialidase A across BV groups and genotypes was observed. Finally, the study did not find a significant association (p>0.05) between reported symptoms of abnormal vaginal discharge and genotype harboured. Conclusion. This study provided the first report on the diversity of G. vaginalis in South African pregnant women. Diversity assessments of G. vaginalis with respect to genotypes and virulence factors may aid in a greater understanding of the pathogenesis of this microorganism.


---

## Body

## 1. Background

Bacterial vaginosis (BV) is an imbalance of the vaginal microenvironment [1]. The condition is characterized by a lower abundance of “healthy” lactobacilli and overgrowth of diverse anaerobic bacteria such as Gardnerella, Atopobium, Mobiluncus, Prevotella, Bacteroides, Anaerococcus, Peptostreptococcus, Sneathia, and Leptotrichia and members of the class Clostridia [2]. Bacterial vaginosis has been associated with preterm birth and poor perinatal outcomes [3]. A strong association between BV and sexually transmitted infections (STIs) has also been reported [4].Gardnerella vaginalis is found in most women with vaginosis and has been reported to be the main cause of clinical signs and symptoms used to diagnose BV [2, 5]. G. vaginalis was originally discovered by Leopold [6] who described this microorganism as a “Haemophilus-like” species associated with prostatitis and cervicitis. G. vaginalis has the necessary virulence factors including production of sialidase which aids in the adherence to the host vaginal epithelium in order to compete with normal vaginal flora for dominance [7]. Sialidase produced by G. vaginalis degrades mucosal sialoglycans, believed to be important in BV [8]. Additionally, sialidase produced by some strains of G. vaginalis has been shown to interfere with host immune modulation resulting in adverse pregnancy outcomes [9].For over three decades, researchers have been conducting extensive bacterial typing assays, in order to identify different virulence traits amongGardnerella spp. [10]. Phenotypic assays have been used to assess the diversity of Gardnerella spp. based on their biochemical properties such as production of b-galactosidase, lipase, and hippurate hydrolysis. However, the early typing assays had failed to reveal the diversity of G. vaginalis5 [8],. The genetic heterogeneity of G. vaginalis species has been determined using molecular approaches, such as Amplified Ribosomal DNA restriction analysis (ARDRA) [11]. ARDRA is a simple, fast, and reproducible method for microbial molecular epidemiology and taxonomy [12]. The ARDRA genotyping approach developed by Balashov and coworkers was shown to be less error-prone [13]. In the study by Ingianni et al. [14], the ARDRA method allowed for G. vaginalis to be separated into at least 4 genotypes.Despite the availability of useful genotyping techniques forG. vaginalis, it has been documented that there is limited data on the prevalence of G. vaginalis genotypes from across the globe [15]. This study investigated the diversity of G. vaginalis from noncultured vaginal swabs obtained from pregnant women by ARDRA.Past studies have described a link with sialidase production and particularG. vaginalis ARDRA genotypes [9, 15]. A recent study by our research group had found high copy numbers of the sialidase A gene across BV-intermediate and BV-positive women and in women with and without abnormal vaginal discharge (unpublished). However, the association between sialidase and G. vaginalis ARDRA genotypes was not performed. This current study will attempt to fill this gap in evidence. In addition, no clear association between BV and any of the ARDRA genotypes has been reported [11]. Through this study, the distribution of G. vaginalis ARDRA genotypes linked to BV status and clinical symptoms of BV such as abnormal vaginal discharge will be determined.


## 2. Methodology

### 2.1. Ethics Approval

The study was approved by the Biomedical Research Ethics Committee (BREC) of the University of KwaZulu-Natal (BREC/00000093/2019).


### 2.2. Study Population

A population of pregnant women was recruited from the King Edward VIII hospital in Durban, South Africa. The study population was recruited from October 2017 to April 2018. The enrolled women (n=354) provided self-collected vaginal swabs after receiving instructions from the study staff on the method of sample collection. Samples were collected from women from gestational age 12 weeks to 37 weeks. The women were classified as BV-negative, BV-intermediate, and BV-positive using the Nugent scoring criteria on gram-stained vaginal smears. A 100% in-house quality control check on the gram-stained slides was performed. The study was conducted at the School of Clinical Medicine Research Laboratory at the Nelson R. Mandela School of Medicine, University of KwaZulu-Natal.


### 2.3. Laboratory Procedures

#### 2.3.1. Nugent Scoring for Grading of Vaginal Smears

Smears were prepared from vaginal swabs and rolled onto glass slides. The slides were gram-stained and examined under the oil immersion objective. Each slide was then graded as per the standardized quantitative morphological classification method developed by Nugent et al. [16].


#### 2.3.2. DNA Extraction

DNA was extracted from the vaginal swabs using a commercially available kit, PureLink Microbiome DNA purification kit (ThermoFisher Scientific, Massachusetts, United States), according to the manufacturer’s instructions.


#### 2.3.3. Amplification of the16S Ribosomal RNA of G. vaginalis

The16S rRNA gene specific to G. vaginalis was amplified using primers: forward: 5′-TTCGATTCTGGCTCAGG and reverse: 5′-CCATCCC AAAAGGGTTAGGC. The primers were synthesized based on their published sequences described by Pleckaityte et al. [15]. The PCR was performed in a 50 μL final volume and comprised 0.2 μM of each primer, 30 ng of genomic DNA, and 1.5 U of High-Fidelity PCR enzyme mix (ThermoFisher Scientific, Massachusetts, United States). The reaction mixture was subjected to 28 cycles of denaturation at 94°C for 30 seconds, primer annealing at 52°C for 45 seconds, and extension at 72°C for 1 minute 25 seconds. PCR conditions were as per Pleckaityte et al. [15]. All PCR reactions were performed using a T100 thermocycler (BioRad, California, United States). The PCR products were separated on a 1% agarose gel and viewed under a UV transilluminator (Gene Genius, SYNGENE).


#### 2.3.4. Sequence Analysis of the16S Ribosomal RNA

To confirm the identity of the PCR amplicons prior to genotyping, the amplicons were sequenced using the Sanger method [17] at Inqaba Biotechnological Industries in Pretoria, South Africa. The amplicons were sequenced using an ABI3500XL genetic analyser, and the raw sequence data was edited using Chromas software V2.6.5 (Technelysium, Queensland, Australia). The edited forward and reverse sequences were aligned using the DNAMAN software (Lynnon Biosoft, California, United States), and the identity of the edited sequences was confirmed using the National Center for Biotechnology Information (NCBI) Basic Local Alignment Search Tool (BLAST).


#### 2.3.5. Amplified Ribosomal DNA Restriction Analysis (ARDRA)

The PCR products were subjected to restriction analysis withTaqI, BamHI, and HindIII (New England Biolabs, Massachusetts, United States). Digestion with TaqI was performed at 65°C for 3 hours, and digestion with BamHI and HindIII was performed at 37°C for 4 hours. Restriction products were analysed on a 1.5% agarose gel (SeaKem LE Agarose-Lonza, Maine, United States) electrophoresed in Tris-borate-EDTA (TBE) buffer.


#### 2.3.6. Phylogenetic Analysis of Genotypes

A phylogenetic tree was then constructed from the16S rRNA sequence data using the Molecular Evolutionary Genetics Analysis (MEGA) version 10 software (Arizona, United States). A bootstrap consensus tree inferred from 100 replicates using the Neighbour Joining method was generated [18].


#### 2.3.7. Detection of theSialidase A Gene from G. vaginalis

The presence of thesialidase A gene was detected using the following specific primers: forward: 5′-GACGACGGCGAATGGCACGA-3′ and reverse: 5′-TACAAGCGGCTTTACTCTTG-3′. The primers were synthesized based on their published sequences described by Pleckaityte et al. [15]. The PCR conditions were as follows: an initial denaturation at 95°C for 10 minutes was followed by 40 cycles of denaturation at 95°C for 30 seconds, annealing was performed at 58°C for 1 minute and extension at 72°C for 2 minutes, and this was followed by final extension at 72°C for 7 minutes. All PCR reactions were performed using a T100 thermocycler (BioRad, California, United States). PCR products were separated on a 1% agarose gel and viewed under a UV transilluminator (Gene Genius, SYNGENE).


#### 2.3.8. Sequence Analysis ofSialidase A

Amplicons generated using thesialidase A-specific primers were sequenced using the Sanger method as previously described. The sequence data generated for the sialidase A gene was compared across the genotypes.


#### 2.3.9. Data Analysis

The data analysis was performed in R Statistical Computing software, version 3.6.2. To assess the association between the symptoms and the BV status for each genotype, the Chi-squared goodness of fit test for one sample was used. The results were also presented as component bar charts.


## 2.1. Ethics Approval

The study was approved by the Biomedical Research Ethics Committee (BREC) of the University of KwaZulu-Natal (BREC/00000093/2019).


## 2.2. Study Population

A population of pregnant women was recruited from the King Edward VIII hospital in Durban, South Africa. The study population was recruited from October 2017 to April 2018. The enrolled women (n=354) provided self-collected vaginal swabs after receiving instructions from the study staff on the method of sample collection. Samples were collected from women from gestational age 12 weeks to 37 weeks. The women were classified as BV-negative, BV-intermediate, and BV-positive using the Nugent scoring criteria on gram-stained vaginal smears. A 100% in-house quality control check on the gram-stained slides was performed. The study was conducted at the School of Clinical Medicine Research Laboratory at the Nelson R. Mandela School of Medicine, University of KwaZulu-Natal.


## 2.3. Laboratory Procedures

### 2.3.1. Nugent Scoring for Grading of Vaginal Smears

Smears were prepared from vaginal swabs and rolled onto glass slides. The slides were gram-stained and examined under the oil immersion objective. Each slide was then graded as per the standardized quantitative morphological classification method developed by Nugent et al. [16].


### 2.3.2. DNA Extraction

DNA was extracted from the vaginal swabs using a commercially available kit, PureLink Microbiome DNA purification kit (ThermoFisher Scientific, Massachusetts, United States), according to the manufacturer’s instructions.


### 2.3.3. Amplification of the16S Ribosomal RNA of G. vaginalis

The16S rRNA gene specific to G. vaginalis was amplified using primers: forward: 5′-TTCGATTCTGGCTCAGG and reverse: 5′-CCATCCC AAAAGGGTTAGGC. The primers were synthesized based on their published sequences described by Pleckaityte et al. [15]. The PCR was performed in a 50 μL final volume and comprised 0.2 μM of each primer, 30 ng of genomic DNA, and 1.5 U of High-Fidelity PCR enzyme mix (ThermoFisher Scientific, Massachusetts, United States). The reaction mixture was subjected to 28 cycles of denaturation at 94°C for 30 seconds, primer annealing at 52°C for 45 seconds, and extension at 72°C for 1 minute 25 seconds. PCR conditions were as per Pleckaityte et al. [15]. All PCR reactions were performed using a T100 thermocycler (BioRad, California, United States). The PCR products were separated on a 1% agarose gel and viewed under a UV transilluminator (Gene Genius, SYNGENE).


### 2.3.4. Sequence Analysis of the16S Ribosomal RNA

To confirm the identity of the PCR amplicons prior to genotyping, the amplicons were sequenced using the Sanger method [17] at Inqaba Biotechnological Industries in Pretoria, South Africa. The amplicons were sequenced using an ABI3500XL genetic analyser, and the raw sequence data was edited using Chromas software V2.6.5 (Technelysium, Queensland, Australia). The edited forward and reverse sequences were aligned using the DNAMAN software (Lynnon Biosoft, California, United States), and the identity of the edited sequences was confirmed using the National Center for Biotechnology Information (NCBI) Basic Local Alignment Search Tool (BLAST).


### 2.3.5. Amplified Ribosomal DNA Restriction Analysis (ARDRA)

The PCR products were subjected to restriction analysis withTaqI, BamHI, and HindIII (New England Biolabs, Massachusetts, United States). Digestion with TaqI was performed at 65°C for 3 hours, and digestion with BamHI and HindIII was performed at 37°C for 4 hours. Restriction products were analysed on a 1.5% agarose gel (SeaKem LE Agarose-Lonza, Maine, United States) electrophoresed in Tris-borate-EDTA (TBE) buffer.


### 2.3.6. Phylogenetic Analysis of Genotypes

A phylogenetic tree was then constructed from the16S rRNA sequence data using the Molecular Evolutionary Genetics Analysis (MEGA) version 10 software (Arizona, United States). A bootstrap consensus tree inferred from 100 replicates using the Neighbour Joining method was generated [18].


### 2.3.7. Detection of theSialidase A Gene from G. vaginalis

The presence of thesialidase A gene was detected using the following specific primers: forward: 5′-GACGACGGCGAATGGCACGA-3′ and reverse: 5′-TACAAGCGGCTTTACTCTTG-3′. The primers were synthesized based on their published sequences described by Pleckaityte et al. [15]. The PCR conditions were as follows: an initial denaturation at 95°C for 10 minutes was followed by 40 cycles of denaturation at 95°C for 30 seconds, annealing was performed at 58°C for 1 minute and extension at 72°C for 2 minutes, and this was followed by final extension at 72°C for 7 minutes. All PCR reactions were performed using a T100 thermocycler (BioRad, California, United States). PCR products were separated on a 1% agarose gel and viewed under a UV transilluminator (Gene Genius, SYNGENE).


### 2.3.8. Sequence Analysis ofSialidase A

Amplicons generated using thesialidase A-specific primers were sequenced using the Sanger method as previously described. The sequence data generated for the sialidase A gene was compared across the genotypes.


### 2.3.9. Data Analysis

The data analysis was performed in R Statistical Computing software, version 3.6.2. To assess the association between the symptoms and the BV status for each genotype, the Chi-squared goodness of fit test for one sample was used. The results were also presented as component bar charts.


## 2.3.1. Nugent Scoring for Grading of Vaginal Smears

Smears were prepared from vaginal swabs and rolled onto glass slides. The slides were gram-stained and examined under the oil immersion objective. Each slide was then graded as per the standardized quantitative morphological classification method developed by Nugent et al. [16].


## 2.3.2. DNA Extraction

DNA was extracted from the vaginal swabs using a commercially available kit, PureLink Microbiome DNA purification kit (ThermoFisher Scientific, Massachusetts, United States), according to the manufacturer’s instructions.


## 2.3.3. Amplification of the16S Ribosomal RNA of G. vaginalis

The16S rRNA gene specific to G. vaginalis was amplified using primers: forward: 5′-TTCGATTCTGGCTCAGG and reverse: 5′-CCATCCC AAAAGGGTTAGGC. The primers were synthesized based on their published sequences described by Pleckaityte et al. [15]. The PCR was performed in a 50 μL final volume and comprised 0.2 μM of each primer, 30 ng of genomic DNA, and 1.5 U of High-Fidelity PCR enzyme mix (ThermoFisher Scientific, Massachusetts, United States). The reaction mixture was subjected to 28 cycles of denaturation at 94°C for 30 seconds, primer annealing at 52°C for 45 seconds, and extension at 72°C for 1 minute 25 seconds. PCR conditions were as per Pleckaityte et al. [15]. All PCR reactions were performed using a T100 thermocycler (BioRad, California, United States). The PCR products were separated on a 1% agarose gel and viewed under a UV transilluminator (Gene Genius, SYNGENE).


## 2.3.4. Sequence Analysis of the16S Ribosomal RNA

To confirm the identity of the PCR amplicons prior to genotyping, the amplicons were sequenced using the Sanger method [17] at Inqaba Biotechnological Industries in Pretoria, South Africa. The amplicons were sequenced using an ABI3500XL genetic analyser, and the raw sequence data was edited using Chromas software V2.6.5 (Technelysium, Queensland, Australia). The edited forward and reverse sequences were aligned using the DNAMAN software (Lynnon Biosoft, California, United States), and the identity of the edited sequences was confirmed using the National Center for Biotechnology Information (NCBI) Basic Local Alignment Search Tool (BLAST).


## 2.3.5. Amplified Ribosomal DNA Restriction Analysis (ARDRA)

The PCR products were subjected to restriction analysis withTaqI, BamHI, and HindIII (New England Biolabs, Massachusetts, United States). Digestion with TaqI was performed at 65°C for 3 hours, and digestion with BamHI and HindIII was performed at 37°C for 4 hours. Restriction products were analysed on a 1.5% agarose gel (SeaKem LE Agarose-Lonza, Maine, United States) electrophoresed in Tris-borate-EDTA (TBE) buffer.


## 2.3.6. Phylogenetic Analysis of Genotypes

A phylogenetic tree was then constructed from the16S rRNA sequence data using the Molecular Evolutionary Genetics Analysis (MEGA) version 10 software (Arizona, United States). A bootstrap consensus tree inferred from 100 replicates using the Neighbour Joining method was generated [18].


## 2.3.7. Detection of theSialidase A Gene from G. vaginalis

The presence of thesialidase A gene was detected using the following specific primers: forward: 5′-GACGACGGCGAATGGCACGA-3′ and reverse: 5′-TACAAGCGGCTTTACTCTTG-3′. The primers were synthesized based on their published sequences described by Pleckaityte et al. [15]. The PCR conditions were as follows: an initial denaturation at 95°C for 10 minutes was followed by 40 cycles of denaturation at 95°C for 30 seconds, annealing was performed at 58°C for 1 minute and extension at 72°C for 2 minutes, and this was followed by final extension at 72°C for 7 minutes. All PCR reactions were performed using a T100 thermocycler (BioRad, California, United States). PCR products were separated on a 1% agarose gel and viewed under a UV transilluminator (Gene Genius, SYNGENE).


## 2.3.8. Sequence Analysis ofSialidase A

Amplicons generated using thesialidase A-specific primers were sequenced using the Sanger method as previously described. The sequence data generated for the sialidase A gene was compared across the genotypes.


## 2.3.9. Data Analysis

The data analysis was performed in R Statistical Computing software, version 3.6.2. To assess the association between the symptoms and the BV status for each genotype, the Chi-squared goodness of fit test for one sample was used. The results were also presented as component bar charts.


## 3. Results

### 3.1. BV Diagnosis

Of the 354 samples analysed, 124 were BV-positive, 37 were BV-intermediate, and 113 were BV-negative. The remaining slides (100) were unreadable due to poor quality of the slide (inadequate sample material on slide). We randomly selected 50 BV-negative, 37 BV-intermediate, and 50 BV-positive specimens for the genotypic analysis. A total of 137 samples were analysed.


### 3.2. Amplification of the16S Ribosomal RNA of G. vaginalis

The 1300 bp fragment corresponding to the16S rRNA of G. vaginalis was only amplified in 37 of the 137 samples analysed (27.2%) (data not shown). The 16S rRNA gene was not detected in any of the BV-negative samples (0/50). A BV-negative group was therefore not included in any further analysis. From the 50 BV-positive samples based on Nugent scoring, only 23 samples produced the 1300 bp product. In addition, 14/37 BV-intermediate samples were amplifiable. Attempts to generate amplicons for the unsuccessful samples were investigated such as increasing the concentration of template DNA and adjusting primer and amplification conditions. All attempts were unsuccessful. The possibility of sample inhibitors affecting the PCR reactions or failed DNA extractions was ruled out since the same DNA samples were amplifiable for other genes not included in this study. A set of 37 samples were used for further analysis. The DNA sequencing hits of the 16S rRNA showed identity (97%) to G. vaginalis strain GS10234 (MH898659.1) and G. vaginalis strain N153 (98%) (JQ354973.1).


### 3.3. Genotyping Analysis

The distribution of the genotypes based onTaqI digestion for the 37 specimens analysed is shown in Table 1. The subtypes of the genotypes which were determined by combining the banding profiles of BamHI and HindIII digestions are also presented in Table 1.Table 1
Genotypes identified after digestion withTaqI across BV-intermediate and BV-positive women. Subtypes identified after digestion with BamHI and HindIII.
Sample nameTaqI fragment sizesGenotypeBamHI fragment sizesHindIII fragment sizesSubtypeBV positivesV003100 bp, 200 bp, 350 bp, 500 bp2200 bp, 1100 bp1200 bp2BV010350 bp, 400 bp11200 bp1200 bp1V018Bands not visible—Bands not visibleBands not visible—V021100 bp, 350 bp, 400 bp, 500 bp1200 bp, 1100 bp1200 bp2BV027100 bp, 350 bp, 400 bp, 500 bp11200 bp1200 bp1V031100 bp, 350 bp, 400 bp, 500 bp1200 bp, 1100 bp1200 bp2BV032250 bp, 350 bp, 400 bp, 500 bp1200 bp, 1100 bp450 bp, 1000 bp, 1200 bp2ABV035350 bp, 500 bp2200 bp, 1100 bp1200 bp2BV042400 bp, 500 bp1200 bp, 1100 bp1200 bp2BV044400 bp, 500 bp1200 bp, 1100 bp450 bp, 1000 bp2CV050100 bp, 200 bp, 350 bp, 500 bp2200 bp, 1100 bp1200 bp2BV055100 bp, 350 bp, 400 bp, 500 bp1200 bp, 1100 bp450 bp, 1000 bp2CV057100 bp, 350 bp, 400 bp, 500 bp1200 bp, 1100 bp450 bp, 1000 bp2CV069100 bp, 200 bp, 350 bp, 500 bp2200 bp, 1100 bp1200 bp2BV081100 bp, 350 bp, 400 bp, 500 bp1200 bp, 1100 bp1200 bp2BV084100 bp, 200 bp, 350 bp, 500 bp2200 bp, 1100 bp450 bp, 1000 bp, 1200 bp2ABV088350 bp, 500 bp2200 bp, 1100 bp1200 bp2BV092100 bp, 350 bp, 400 bp, 500 bp1200 bp, 1100 bp450 bp, 1000 bp, 1200 bp2ABV098500 bpDifferent patterns200 bp, 1100 bp1200 bp2BV103500 bpDifferent patterns200 bp, 1100 bp450 bp, 1000 bp 1200 bp2ABV121Bands not visible—Bands not visibleBands not visible—V124400 bp, 500 bp1200 bp, 1100 bpBands not visible—V126100 bp, 350 bp, 400 bp, 500 bp1200 bp, 1100 bp1200 bp2BBV intermediatesV056250 bp, 400 bp, 500 bp11200 bp1200 bp1V085Undigested DNA—1200 bp1200 bp1V095250 bp, 350 bp, 400 bp, 500 bp1200 bp, 1100 bp1200 bp1V096250 bp, 400 bp, 500 bp1200 bp, 1100 bp1200 bp2BV102Very faint pattern—1200 bp1200 bp1V184No band visible—1200 bp1200 bp1V202250 bp, 350 bp, 500 bp2200 bp, 1100 bp1200 bp1V206250 bp, 350 bp, 500 bp21200 bp1200 bp1V207250 bp, 350 bp, 500 bp2200 bp, 1100 bp1200 bp2BV239250 bp, 350 bp, 400 bp, 500 bp1200 bp, 1100 bp400 bp, 1000 bp2BV240250 bp, 400 bp, 500 bp1200 bp, 1100 bp1200 bp2BV258250 bp, 400 bp, 500 bp1200 bp, 1100 bp450 bp, 1000 bp2CV269No bands visible—1200 bp1200 bp1V281250 bp, 400 bp, 500 bp11200 bp1200 bp1


#### 3.3.1. Genotypes Based onTaqI Digestion

Restriction digestion withTaqI revealed the presence of two different genotypes, i.e., GT1 and GT2. GT1 was carried by 20/37 specimens (54%), followed by GT2 which was present in 9/37 specimens (24%). Of the 37 specimens analysed, 7 specimens were not ascribed genotypes. Two of the specimens from the BV-positive sample group produced a banding profile (i.e., a single band at 500 bp) that was not described in previously published studies. One specimen from the BV-intermediate group produced a very faint profile which was difficult to interpret. The remaining 3 specimens did not produce any bands; the gel lanes appeared blank for those samples. These specimens were across both BV status groups.Within the BV-positive sample group, 13/23 specimens carried GT1 (57%) and 6 of the 23 specimens (26%) carried GT2. Two specimens produced a differing banding profile (9%), and 2 specimens did not produce any bands (9%) (Figure1).Figure 1
Profiles of BV-positive samples digested withTaqI. Lane M: 100 bp DNA molecular ladder (ThermoFisher Scientific). Genotypes 1 (100 bp, 350 bp, 400 bp, and 500 bp banding patterns) and 2 (100 bp, 200 bp, 350 bp, and 500 bp banding patterns) were distributed across BV-positive samples.A similar profile was observed for the BV-intermediate sample group; a larger number of specimens carried GT1 (7/14, 50%), and 3 out of 14 samples carried GT2 (21%). Three samples did not produce visible bands (21%) (Figure2).Figure 2
Profiles of BV-intermediate samples digested withTaqI. Lane M: 100 bp DNA molecular ladder (ThermoFisher Scientific). Genotypes 1 (100 bp, 350 bp, 400 bp, and 500 bp banding patterns) and 2 (100 bp, 200 bp, 350 bp, and 500 bp banding patterns) were distributed across BV-intermediate samples.


#### 3.3.2. Subtypes Based onBamHI and HindIII Digestions

Within the BV-positive sample group, all 4 subtypes were observed. Subtype 2B was highly prevalent with 11/23 (48%) specimens harbouring this subtype followed by the mixed 2AB subtype (4/23, 17%), subtype 2C (3/23, 13%), and subtype 1 (2/23, 9%) (Figure3). In the sample group that carried GT1 (n=13), 5 specimens harboured subtype 2B (39%), 3 with subtype 2C (23%), 2 with subtype 2AB (15%), and 2 with subtype 1 (15%) (Table 1). Subtype 2B was highly prevalent in the sample group carrying GT2 (5/6, 83%), followed by subtype 2AB (1/6, 17%). Subtypes 1 and 2C were not present in this genotypic group (Table 1).Figure 3
Subtypes assigned to BV-positive samples based onBamHI and HindIII digestion profiles. Lane M: 100 bp DNA molecular ladder (ThermoFisher Scientific). Subtypes 1 (1200 bp band for BamHI and HindIII digestions), 2B (200 bp and 1100 bp for BamHI digestion and 1200 bp for HindIII digestion), 2AB (200 bp and 1100 bp for BamHI digestion and 450 bp, 1000 bp, and 1200 bp for HindIII digestion), and 2C (200 bp and 1100 bp for BamHI digestion and 450 bp and 1000 bp for HindIII digestion) were distributed across the BV-positive samples.Within the BV-intermediate sample group, 3 subtypes were observed (subtypes, 1, 2B, and 2C) (Figure4). The most prevalent subtype in this group was subtype 1 (9/14, 64%) followed by subtype 2B (4/9, 44%) and subtype 2C (1/9, 11%). Subtypes 1 (3/7, 43%) and 2B (3/7, 43%) were most prevalent in GT1 specimens. One specimen in this genotypic group carried subtype 2C (7%) (Table 1). Subtype 1 was also shown to be most prevalent in the GT2 specimens (2/3, 67%) followed by subtype 2B (1/3, 33%). Subtype 2C was not present in this genotypic group (Table 1).Figure 4
Subtypes assigned to BV-intermediate samples based onBamHI and HindIII digestion profiles. Lane M: 100 bp DNA molecular ladder (ThermoFisher Scientific). Subtypes 1 (1200 bp band for BamHI and HindIII digestions), 2B (200 bp and 1100 bp for BamHI digestion and 1200 bp for HindIII digestion), and 2C (200 bp and 1100 bp for BamHI digestion and 450 bp and 1000 bp for HindIII digestion) were distributed across the BV-intermediate samples.


### 3.4. Phylogenetic Analysis of16S rRNA Genotypes and Subtypes

The phylogenetic tree revealed the presence of 5 sequence clusters (Figure5). The tree displayed clusters which contained groups of specimens from a particular BV group (clusters 1, 3, and 5). Within these same BV groups, there were however differences noted for either the genotypes assigned and/or subtypes present. Additionally, there were clusters which contained specimens from across both BV groups such as clusters 2 and 4. Despite the heterogeneity with respect to the BV group, cluster 2 contained specimens of the same genotype (GT1) with the majority carrying the same subtype (S2B). Cluster 4 on the other hand contained specimens of the same genotype (GT1) with a combination of all 4 subtypes (S1, S2B, S2AB, and S2C).Figure 5
Phylogenetic analysis according to distribution of genotypes. The tree was constructed using the Neighbour Joining method. The optimal tree with the sum of branchlength=15.35302543 is shown. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (100 replicates) is shown next to the branches. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the Maximum Composite Likelihood method and are in the units of the number of base substitutions per site. Evolutionary analyses were conducted in MEGA X [18].


### 3.5. Symptoms Associated with BV across Genotypes and BV States

The median age (Q1-Q3) of the women in the BV-intermediate group was 26.5 (21.3-28.8), and the median age (Q1-Q3) of the women in the BV-positive group was 26.0 (24.0-30.5). Among the BV-intermediate and BV-positive groups, a higher percentage of the women did not present with symptoms of abnormal vaginal discharge (i.e., were asymptomatic). For BV-intermediate and BV-positive women harbouring GT1, there was no significant difference in women who reported abnormal vaginal discharge when compared to women who did not report the discharge (p>0.05) (Figure 6(a)).Figure 6
Symptoms of bacterial vaginosis across the intermediate and positive women in relation to genotypes. ns = not significant.
(a)(b)Similarly, for the women harbouring GT2, there was no significant difference in the BV-positive women who reported abnormal vaginal discharge when compared to women who did not report discharge (p>0.05). All BV-intermediate women with GT2 reported no symptoms of abnormal vaginal discharge (Figure 6(b)).


### 3.6. Sequence Diversity ofSialidase A Linked to the G. vaginalis Genotypes

Thesialidase A gene was detected in both BV-intermediate and BV-positive women. A subset of sialidase A-positive amplicons from samples representing the different genotypes across the BV groups was sequenced and analysed. Four sialidase A sequence clusters were observed (Figure 7). Cluster 1 contained sequences from BV-intermediate samples harbouring the same genotype (GT2) but different subtypes. Cluster 2 contained sequences from BV-positive samples harbouring the same genotype (GT1) but different subtypes. Cluster 3 was a heterogeneous group containing sequences from both BV-intermediate and BV-positive groups harbouring different genotypes but the same subtype. Lastly, cluster 4 contained sequences from BV-intermediate samples harbouring the different genotypes but the same subtype. Overall, diversity of the sialidase A in terms of BV group and genotypes harboured was observed.Figure 7
Phylogenetic analysis ofsialidase A in relation to genotype. The tree was constructed using the Neighbour Joining method. The evolutionary history was inferred using the Neighbour Joining method. The optimal tree with the sum of branch length=0.18320599 is shown. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (100 replicates) is shown next to the branches. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using evolutionary analyses which were conducted in MEGA X [18].


## 3.1. BV Diagnosis

Of the 354 samples analysed, 124 were BV-positive, 37 were BV-intermediate, and 113 were BV-negative. The remaining slides (100) were unreadable due to poor quality of the slide (inadequate sample material on slide). We randomly selected 50 BV-negative, 37 BV-intermediate, and 50 BV-positive specimens for the genotypic analysis. A total of 137 samples were analysed.


## 3.2. Amplification of the16S Ribosomal RNA of G. vaginalis

The 1300 bp fragment corresponding to the16S rRNA of G. vaginalis was only amplified in 37 of the 137 samples analysed (27.2%) (data not shown). The 16S rRNA gene was not detected in any of the BV-negative samples (0/50). A BV-negative group was therefore not included in any further analysis. From the 50 BV-positive samples based on Nugent scoring, only 23 samples produced the 1300 bp product. In addition, 14/37 BV-intermediate samples were amplifiable. Attempts to generate amplicons for the unsuccessful samples were investigated such as increasing the concentration of template DNA and adjusting primer and amplification conditions. All attempts were unsuccessful. The possibility of sample inhibitors affecting the PCR reactions or failed DNA extractions was ruled out since the same DNA samples were amplifiable for other genes not included in this study. A set of 37 samples were used for further analysis. The DNA sequencing hits of the 16S rRNA showed identity (97%) to G. vaginalis strain GS10234 (MH898659.1) and G. vaginalis strain N153 (98%) (JQ354973.1).


## 3.3. Genotyping Analysis

The distribution of the genotypes based onTaqI digestion for the 37 specimens analysed is shown in Table 1. The subtypes of the genotypes which were determined by combining the banding profiles of BamHI and HindIII digestions are also presented in Table 1.Table 1
Genotypes identified after digestion withTaqI across BV-intermediate and BV-positive women. Subtypes identified after digestion with BamHI and HindIII.
Sample nameTaqI fragment sizesGenotypeBamHI fragment sizesHindIII fragment sizesSubtypeBV positivesV003100 bp, 200 bp, 350 bp, 500 bp2200 bp, 1100 bp1200 bp2BV010350 bp, 400 bp11200 bp1200 bp1V018Bands not visible—Bands not visibleBands not visible—V021100 bp, 350 bp, 400 bp, 500 bp1200 bp, 1100 bp1200 bp2BV027100 bp, 350 bp, 400 bp, 500 bp11200 bp1200 bp1V031100 bp, 350 bp, 400 bp, 500 bp1200 bp, 1100 bp1200 bp2BV032250 bp, 350 bp, 400 bp, 500 bp1200 bp, 1100 bp450 bp, 1000 bp, 1200 bp2ABV035350 bp, 500 bp2200 bp, 1100 bp1200 bp2BV042400 bp, 500 bp1200 bp, 1100 bp1200 bp2BV044400 bp, 500 bp1200 bp, 1100 bp450 bp, 1000 bp2CV050100 bp, 200 bp, 350 bp, 500 bp2200 bp, 1100 bp1200 bp2BV055100 bp, 350 bp, 400 bp, 500 bp1200 bp, 1100 bp450 bp, 1000 bp2CV057100 bp, 350 bp, 400 bp, 500 bp1200 bp, 1100 bp450 bp, 1000 bp2CV069100 bp, 200 bp, 350 bp, 500 bp2200 bp, 1100 bp1200 bp2BV081100 bp, 350 bp, 400 bp, 500 bp1200 bp, 1100 bp1200 bp2BV084100 bp, 200 bp, 350 bp, 500 bp2200 bp, 1100 bp450 bp, 1000 bp, 1200 bp2ABV088350 bp, 500 bp2200 bp, 1100 bp1200 bp2BV092100 bp, 350 bp, 400 bp, 500 bp1200 bp, 1100 bp450 bp, 1000 bp, 1200 bp2ABV098500 bpDifferent patterns200 bp, 1100 bp1200 bp2BV103500 bpDifferent patterns200 bp, 1100 bp450 bp, 1000 bp 1200 bp2ABV121Bands not visible—Bands not visibleBands not visible—V124400 bp, 500 bp1200 bp, 1100 bpBands not visible—V126100 bp, 350 bp, 400 bp, 500 bp1200 bp, 1100 bp1200 bp2BBV intermediatesV056250 bp, 400 bp, 500 bp11200 bp1200 bp1V085Undigested DNA—1200 bp1200 bp1V095250 bp, 350 bp, 400 bp, 500 bp1200 bp, 1100 bp1200 bp1V096250 bp, 400 bp, 500 bp1200 bp, 1100 bp1200 bp2BV102Very faint pattern—1200 bp1200 bp1V184No band visible—1200 bp1200 bp1V202250 bp, 350 bp, 500 bp2200 bp, 1100 bp1200 bp1V206250 bp, 350 bp, 500 bp21200 bp1200 bp1V207250 bp, 350 bp, 500 bp2200 bp, 1100 bp1200 bp2BV239250 bp, 350 bp, 400 bp, 500 bp1200 bp, 1100 bp400 bp, 1000 bp2BV240250 bp, 400 bp, 500 bp1200 bp, 1100 bp1200 bp2BV258250 bp, 400 bp, 500 bp1200 bp, 1100 bp450 bp, 1000 bp2CV269No bands visible—1200 bp1200 bp1V281250 bp, 400 bp, 500 bp11200 bp1200 bp1


### 3.3.1. Genotypes Based onTaqI Digestion

Restriction digestion withTaqI revealed the presence of two different genotypes, i.e., GT1 and GT2. GT1 was carried by 20/37 specimens (54%), followed by GT2 which was present in 9/37 specimens (24%). Of the 37 specimens analysed, 7 specimens were not ascribed genotypes. Two of the specimens from the BV-positive sample group produced a banding profile (i.e., a single band at 500 bp) that was not described in previously published studies. One specimen from the BV-intermediate group produced a very faint profile which was difficult to interpret. The remaining 3 specimens did not produce any bands; the gel lanes appeared blank for those samples. These specimens were across both BV status groups.Within the BV-positive sample group, 13/23 specimens carried GT1 (57%) and 6 of the 23 specimens (26%) carried GT2. Two specimens produced a differing banding profile (9%), and 2 specimens did not produce any bands (9%) (Figure1).Figure 1
Profiles of BV-positive samples digested withTaqI. Lane M: 100 bp DNA molecular ladder (ThermoFisher Scientific). Genotypes 1 (100 bp, 350 bp, 400 bp, and 500 bp banding patterns) and 2 (100 bp, 200 bp, 350 bp, and 500 bp banding patterns) were distributed across BV-positive samples.A similar profile was observed for the BV-intermediate sample group; a larger number of specimens carried GT1 (7/14, 50%), and 3 out of 14 samples carried GT2 (21%). Three samples did not produce visible bands (21%) (Figure2).Figure 2
Profiles of BV-intermediate samples digested withTaqI. Lane M: 100 bp DNA molecular ladder (ThermoFisher Scientific). Genotypes 1 (100 bp, 350 bp, 400 bp, and 500 bp banding patterns) and 2 (100 bp, 200 bp, 350 bp, and 500 bp banding patterns) were distributed across BV-intermediate samples.


### 3.3.2. Subtypes Based onBamHI and HindIII Digestions

Within the BV-positive sample group, all 4 subtypes were observed. Subtype 2B was highly prevalent with 11/23 (48%) specimens harbouring this subtype followed by the mixed 2AB subtype (4/23, 17%), subtype 2C (3/23, 13%), and subtype 1 (2/23, 9%) (Figure3). In the sample group that carried GT1 (n=13), 5 specimens harboured subtype 2B (39%), 3 with subtype 2C (23%), 2 with subtype 2AB (15%), and 2 with subtype 1 (15%) (Table 1). Subtype 2B was highly prevalent in the sample group carrying GT2 (5/6, 83%), followed by subtype 2AB (1/6, 17%). Subtypes 1 and 2C were not present in this genotypic group (Table 1).Figure 3
Subtypes assigned to BV-positive samples based onBamHI and HindIII digestion profiles. Lane M: 100 bp DNA molecular ladder (ThermoFisher Scientific). Subtypes 1 (1200 bp band for BamHI and HindIII digestions), 2B (200 bp and 1100 bp for BamHI digestion and 1200 bp for HindIII digestion), 2AB (200 bp and 1100 bp for BamHI digestion and 450 bp, 1000 bp, and 1200 bp for HindIII digestion), and 2C (200 bp and 1100 bp for BamHI digestion and 450 bp and 1000 bp for HindIII digestion) were distributed across the BV-positive samples.Within the BV-intermediate sample group, 3 subtypes were observed (subtypes, 1, 2B, and 2C) (Figure4). The most prevalent subtype in this group was subtype 1 (9/14, 64%) followed by subtype 2B (4/9, 44%) and subtype 2C (1/9, 11%). Subtypes 1 (3/7, 43%) and 2B (3/7, 43%) were most prevalent in GT1 specimens. One specimen in this genotypic group carried subtype 2C (7%) (Table 1). Subtype 1 was also shown to be most prevalent in the GT2 specimens (2/3, 67%) followed by subtype 2B (1/3, 33%). Subtype 2C was not present in this genotypic group (Table 1).Figure 4
Subtypes assigned to BV-intermediate samples based onBamHI and HindIII digestion profiles. Lane M: 100 bp DNA molecular ladder (ThermoFisher Scientific). Subtypes 1 (1200 bp band for BamHI and HindIII digestions), 2B (200 bp and 1100 bp for BamHI digestion and 1200 bp for HindIII digestion), and 2C (200 bp and 1100 bp for BamHI digestion and 450 bp and 1000 bp for HindIII digestion) were distributed across the BV-intermediate samples.


## 3.3.1. Genotypes Based onTaqI Digestion

Restriction digestion withTaqI revealed the presence of two different genotypes, i.e., GT1 and GT2. GT1 was carried by 20/37 specimens (54%), followed by GT2 which was present in 9/37 specimens (24%). Of the 37 specimens analysed, 7 specimens were not ascribed genotypes. Two of the specimens from the BV-positive sample group produced a banding profile (i.e., a single band at 500 bp) that was not described in previously published studies. One specimen from the BV-intermediate group produced a very faint profile which was difficult to interpret. The remaining 3 specimens did not produce any bands; the gel lanes appeared blank for those samples. These specimens were across both BV status groups.Within the BV-positive sample group, 13/23 specimens carried GT1 (57%) and 6 of the 23 specimens (26%) carried GT2. Two specimens produced a differing banding profile (9%), and 2 specimens did not produce any bands (9%) (Figure1).Figure 1
Profiles of BV-positive samples digested withTaqI. Lane M: 100 bp DNA molecular ladder (ThermoFisher Scientific). Genotypes 1 (100 bp, 350 bp, 400 bp, and 500 bp banding patterns) and 2 (100 bp, 200 bp, 350 bp, and 500 bp banding patterns) were distributed across BV-positive samples.A similar profile was observed for the BV-intermediate sample group; a larger number of specimens carried GT1 (7/14, 50%), and 3 out of 14 samples carried GT2 (21%). Three samples did not produce visible bands (21%) (Figure2).Figure 2
Profiles of BV-intermediate samples digested withTaqI. Lane M: 100 bp DNA molecular ladder (ThermoFisher Scientific). Genotypes 1 (100 bp, 350 bp, 400 bp, and 500 bp banding patterns) and 2 (100 bp, 200 bp, 350 bp, and 500 bp banding patterns) were distributed across BV-intermediate samples.


## 3.3.2. Subtypes Based onBamHI and HindIII Digestions

Within the BV-positive sample group, all 4 subtypes were observed. Subtype 2B was highly prevalent with 11/23 (48%) specimens harbouring this subtype followed by the mixed 2AB subtype (4/23, 17%), subtype 2C (3/23, 13%), and subtype 1 (2/23, 9%) (Figure3). In the sample group that carried GT1 (n=13), 5 specimens harboured subtype 2B (39%), 3 with subtype 2C (23%), 2 with subtype 2AB (15%), and 2 with subtype 1 (15%) (Table 1). Subtype 2B was highly prevalent in the sample group carrying GT2 (5/6, 83%), followed by subtype 2AB (1/6, 17%). Subtypes 1 and 2C were not present in this genotypic group (Table 1).Figure 3
Subtypes assigned to BV-positive samples based onBamHI and HindIII digestion profiles. Lane M: 100 bp DNA molecular ladder (ThermoFisher Scientific). Subtypes 1 (1200 bp band for BamHI and HindIII digestions), 2B (200 bp and 1100 bp for BamHI digestion and 1200 bp for HindIII digestion), 2AB (200 bp and 1100 bp for BamHI digestion and 450 bp, 1000 bp, and 1200 bp for HindIII digestion), and 2C (200 bp and 1100 bp for BamHI digestion and 450 bp and 1000 bp for HindIII digestion) were distributed across the BV-positive samples.Within the BV-intermediate sample group, 3 subtypes were observed (subtypes, 1, 2B, and 2C) (Figure4). The most prevalent subtype in this group was subtype 1 (9/14, 64%) followed by subtype 2B (4/9, 44%) and subtype 2C (1/9, 11%). Subtypes 1 (3/7, 43%) and 2B (3/7, 43%) were most prevalent in GT1 specimens. One specimen in this genotypic group carried subtype 2C (7%) (Table 1). Subtype 1 was also shown to be most prevalent in the GT2 specimens (2/3, 67%) followed by subtype 2B (1/3, 33%). Subtype 2C was not present in this genotypic group (Table 1).Figure 4
Subtypes assigned to BV-intermediate samples based onBamHI and HindIII digestion profiles. Lane M: 100 bp DNA molecular ladder (ThermoFisher Scientific). Subtypes 1 (1200 bp band for BamHI and HindIII digestions), 2B (200 bp and 1100 bp for BamHI digestion and 1200 bp for HindIII digestion), and 2C (200 bp and 1100 bp for BamHI digestion and 450 bp and 1000 bp for HindIII digestion) were distributed across the BV-intermediate samples.


## 3.4. Phylogenetic Analysis of16S rRNA Genotypes and Subtypes

The phylogenetic tree revealed the presence of 5 sequence clusters (Figure5). The tree displayed clusters which contained groups of specimens from a particular BV group (clusters 1, 3, and 5). Within these same BV groups, there were however differences noted for either the genotypes assigned and/or subtypes present. Additionally, there were clusters which contained specimens from across both BV groups such as clusters 2 and 4. Despite the heterogeneity with respect to the BV group, cluster 2 contained specimens of the same genotype (GT1) with the majority carrying the same subtype (S2B). Cluster 4 on the other hand contained specimens of the same genotype (GT1) with a combination of all 4 subtypes (S1, S2B, S2AB, and S2C).Figure 5
Phylogenetic analysis according to distribution of genotypes. The tree was constructed using the Neighbour Joining method. The optimal tree with the sum of branchlength=15.35302543 is shown. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (100 replicates) is shown next to the branches. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the Maximum Composite Likelihood method and are in the units of the number of base substitutions per site. Evolutionary analyses were conducted in MEGA X [18].


## 3.5. Symptoms Associated with BV across Genotypes and BV States

The median age (Q1-Q3) of the women in the BV-intermediate group was 26.5 (21.3-28.8), and the median age (Q1-Q3) of the women in the BV-positive group was 26.0 (24.0-30.5). Among the BV-intermediate and BV-positive groups, a higher percentage of the women did not present with symptoms of abnormal vaginal discharge (i.e., were asymptomatic). For BV-intermediate and BV-positive women harbouring GT1, there was no significant difference in women who reported abnormal vaginal discharge when compared to women who did not report the discharge (p>0.05) (Figure 6(a)).Figure 6
Symptoms of bacterial vaginosis across the intermediate and positive women in relation to genotypes. ns = not significant.
(a)(b)Similarly, for the women harbouring GT2, there was no significant difference in the BV-positive women who reported abnormal vaginal discharge when compared to women who did not report discharge (p>0.05). All BV-intermediate women with GT2 reported no symptoms of abnormal vaginal discharge (Figure 6(b)).


## 3.6. Sequence Diversity ofSialidase A Linked to the G. vaginalis Genotypes

Thesialidase A gene was detected in both BV-intermediate and BV-positive women. A subset of sialidase A-positive amplicons from samples representing the different genotypes across the BV groups was sequenced and analysed. Four sialidase A sequence clusters were observed (Figure 7). Cluster 1 contained sequences from BV-intermediate samples harbouring the same genotype (GT2) but different subtypes. Cluster 2 contained sequences from BV-positive samples harbouring the same genotype (GT1) but different subtypes. Cluster 3 was a heterogeneous group containing sequences from both BV-intermediate and BV-positive groups harbouring different genotypes but the same subtype. Lastly, cluster 4 contained sequences from BV-intermediate samples harbouring the different genotypes but the same subtype. Overall, diversity of the sialidase A in terms of BV group and genotypes harboured was observed.Figure 7
Phylogenetic analysis ofsialidase A in relation to genotype. The tree was constructed using the Neighbour Joining method. The evolutionary history was inferred using the Neighbour Joining method. The optimal tree with the sum of branch length=0.18320599 is shown. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (100 replicates) is shown next to the branches. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using evolutionary analyses which were conducted in MEGA X [18].


## 4. Discussion

Gardnerella vaginalis is one of the most frequently isolated microorganisms from women who present with symptoms of BV [19]. High microbial loads of G. vaginalis in the vaginal tract have been linked to reproductive health issues such as infertility and preterm labour [20]. The pathogenesis of G. vaginalis in the vaginal tract is not completely understood since this microorganism has been shown to be present across the BV groups (BV-negative, BV-intermediate, and BV-positive). Differentiation of G. vaginalis strains and subgroups according to sequence variations in 16S rRNA and the cpn60 genes has been made possible using molecular biology approaches [13].In this study, the diversity of theG. vaginalis 16S rRNA was analysed across BV-intermediate and BV-positive pregnant women who were diagnosed by the Nugent method. A BV-negative group was not included in the diversity analysis since none of the BV-negative samples produced a positive PCR amplicon for the 16S rRNA specific for G. vaginalis. However, the presence of Lactobacillus crispatus was shown to be present in the negative specimens eliminating the possibility of a failed DNA extraction or PCR amplification for the negative specimens (data not shown). Our failure to amplify the G. vaginalis 16S rRNA in the negative samples differs from previously published works which had shown the presence of G. vaginalis in BV-negative specimens based on PCR detection of the 16S rRNA gene [13, 21]. However, a fairly recent study conducted by our research group was able to detect G. vaginalis in our BV-negative pregnant cohort using the highly sensitive Droplet Digital PCR (ddPCR) System (submitted for publication). This leads to the assumption that ARDRA may not be a very sensitive method for detecting G. vaginalis directly from clinical samples in women classified as BV-negative. Our assumption is validated by an earlier study conducted by Verhelst et al. [22] which used ARDRA in order to assess the diversity of the vaginal microbiome. In that study, ARDRA failed to identify G. vaginalis in women who were classified as BV-negative; however, G. vaginalis was detected in women who were BV-positive. Despite the suggested limitation, ARDRAhas been useful in identifying different G. vaginalis genotypes [9, 14, 23].Based on the ARDRA technique used in this study, restriction digestion withTaqI revealed the presence of two different genotypes, i.e., GT1 and GT2. Similarly, a study by Pleckaityte et al. [15] reported on the presence of GT1 and GT2 in a population of women with BV in Lithuania. However, the Lithuanian study was unable to detect specific subtypes associated with GT1. All the GT1 sequences in their population of women were identical. However, the present study observed different subtypes associated with GT1. This suggests that a level of diversity does exist between G. vaginalis present in different geographical locations as well as across different population groups (pregnant versus nonpregnant). Additionally, the present study observed a difference in the prevalence of the different subtypes across BV-intermediate and BV-positive women. Among the BV-positive women, the most prevalent subtype was 2B whereas in the BV-intermediate women, the most prevalent subtype was subtype 1, thereby hypothesising a level of genetic differences across BV-intermediate and BV-positive women. Our hypothesis was confirmed by the phylogenetic analysis which showed the presence of 5 sequence clusters on the tree indicating genetic differences across the sequences.The study further investigated the link between genotypes and clinical symptoms of abnormal vaginal discharge. Among the BV-intermediate and BV-positive groups, a higher percentage of the women did not present with symptoms of abnormal vaginal discharge (i.e., were asymptomatic). This study found no significant association between genotypes harboured and symptoms of abnormal vaginal discharge. However, a study conducted by Santiago et al. [9] showed GT2 to be the most prevalent genotype associated with symptomatic BV.This study also investigated the diversity of thesialidase A gene, the virulence factor of G. vaginalis in relation to genotypes. In this study, there was a correlation between the DNA sequences of the sialidase A gene and the respective genotypes. Two of the four sequence clusters contained samples of the same genotype. A clear link between genotype and sialidase production was previously reported by Santiago et al. [9]. However, the present study cannot be directly compared to that of Santiago et al. [9] since the present study detected the sialidase A gene directly from noncultured vaginal swabs whereas Santiago et al. [9] evaluated pure cultures for sialidase activity and diversity. The present study also showed that the diversity of the sialidase A gene was based on the BV group. BV-intermediate and BV-positive sequences formed distinct clusters on the phylogenetic tree indicating a level of diversity of sialidase A gene across the two BV groups. Previous studies on the diversity of sialidase A gene were not investigated in women with intermediate BV. The current study now provides additional data on the diversity of the sialidase A gene for this BV group.The limitations of the study are as follows: the sample size used for the analysis was small. However, despite the small sample size, the study was able to provide data on the prevalent genotypes and subtypes ofG. vaginalis in South African pregnant women across BV-intermediate and BV-positive groups, an area of research which has not been previously investigated in our setting. The study lacked a control group of nonpregnant women which would have been useful to draw comparisons regarding the distribution of the genotypes. The study did not attempt to culture G. vaginalis from the vaginal swabs and therefore did not perform the genotyping assays on pure cultures that would have enabled direct comparisons with previously published studies. However, from the noncultured clinical specimens, the diversity assessments performed still provided substantial evidence. Lastly, due to the cross-sectional nature of the study, we did not associate the genotypes with pregnancy outcomes and acquisition of other infections such as HIV and genital infections. All the limitations described here will be addressed in a study that is planned for commencement in 2021.


## 5. Conclusion

This study provides the first report on the most prevalent genotypes and subtypes ofG. vaginalis across BV-intermediate and BV-positive South African pregnant women. Restriction analysis revealed the presence of two different genotypes, i.e., GT1 and GT2, as well as four subtypes (1, 2B, 2AB, and 2C) circulating in our population. In addition, diversity across the BV groups, genotypes, and subtypes for sialidase A was evident in this study. The observed diversity can be used as a foundation for future studies which are aimed at understanding the pathogenesis of G. vaginalis across BV groups in women from different populations.



---

*Source: 3176407-2020-07-02.xml*