# Quantification of Benazepril Hydrochloride and Hydrochlorothiazide in Tablet Dosage Form by Simultaneous Equation Spectrophotometric Method

Authors: Varsha Parmar; Usmangani Chhalotiya; Dimal Shah; Kashyap Bhatt; Sunil Baldania
Journal: Journal of Applied Chemistry
 (2013)

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## Abstract

Simple, accurate, precise, reproducible, requiring no prior separation, and economical procedures for simultaneous estimation of benazepril hydrochloride (BEN) and Hydrochlorothiazide (HCT)  in tablet dosage form have been developed. Simultaneous equation method employs for estimation of both drugs in methanol at 240 nm and 270 nm as two analytical wavelengths. BEN and HCT at their respectiveλmax (240 nm and 270 nm) show linearity in a concentration range of 2–12 μg/mL and 4–14 μg/mL. Recovery studies for BEN are 100.0–100.6% and 99.8–100.0% for HCT in the case of simultaneous equation method confirming the accuracy of the proposed method. The proposed method is recommended for routine analysis since it is rapid, simple, accurate and also sensitive and specific.


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## Body

## 1. Introduction

Benazepril hydrochloride (BEN) is chemically 3-[[1-(ethoxycarbonyl)-3-phenyl-(1S)-propyl]amino]-2,3,4,5-tetrahydro-2-oxo-1H-1-(3S)-benzazepine-1-acetic acid monohydrochloride (Figure1(a)). The empirical formula of BEN is C24H28N2O5·HCl with a molecular weight of 460.96 g/mole [1]. It is an angiotensin converting enzyme. It is used as antihypertensive agent.(a) Structure of hydrochlorothiazide. (b) Structure of benazepril hydrochloride.
(a)
(b)Hydrochlorothiazide (HCT) is chemically 6-chloro-3,4-dihydro-2H-1,2,4-benzothiadiazine-7-sulfonamide 1,1-dioxide (Figure1(b)). The empirical formula is C7H8ClN3O4S2 with a molecular weight 297.73 g/mole [2]. It is a diuretic agent.BEN and HCT are official in the british Pharmacopeia and the Indian Pharmacopeia. Many methods have been reported in the literature for both determination of BEN, individually and with other drugs in combination [3–17]. However, there is no UV spectrophotometric method reported for the simultaneous estimation of BEN and HCT in pharmaceutical preparations in the literature survey. The objective of the present work is to develop and validate new analytical methods for simultaneous determination of BEN and HCT in tablet dosage form.


## 2.    Experimental

### 2.1. Materials and Methods

A double-beam Shimadzu UV-visible spectrophotometer 1700 (Pharma spec), with wavelength accuracy of ±0.5 nm and a pair of 1 cm matched quartz cells, was used to measure absorbance of the resulting solution. All weighing was done on an electronic balance (Shimadzu BL-220H). All statistical calculations were carried out using Microsoft Excel 2007 analytical tool.Analytically pure BEN and HCT were procured as gift sample from Dishman Pharmaceuticals Ltd. and Cadila Pharmaceutical Pvt. Ltd. (Ahmedabad, India). Methanol (E. Merck, Mumbai, India) analytical grade was used as a diluent. Tablet formulation (Lotensin HCT, Novartis Pharmaceutical Pvt. Ltd.) containing labelled amount of 10 mg of benazepril hydrochloride and 12.5 mg of hydrochlorothiazide was purchased from local market.


### 2.2. Preparation of Solutions

Accurately weighed 100 mg of BEN and HCT standards was transferred to separate 100 mL volumetric flasks and dissolved in 50 mL Methanol. The flasks were shaken, and the volume was made up to the mark with methanol to give solutions containing 1000μg/mL BEN and 1000 μg/mL HCT, respectively.


### 2.3. Methodology

Selection of analytical wavelengths was done by taking pure samples of BEN and HCT, which were separately dissolved in methanol, to give two solutions of 10μg/mL, respectively. They were scanned in the wavelength range of 200–400 nm. From the overlain spectra (Figure 2), wavelengths of  240 and 270 nm were selected for the formation of simultaneous equations. For constructing a calibration curve, two series of different concentrations in range of 2–12 μg/mL for BEN and 4–14 μg/mL for HCT were prepared from stock solutions. The calibration curves were plotted at 240 and 270 nm. The absorptivities (A1%, 1 cm) of both drugs at both wavelengths were determined. These calculated values were the mean of five independent determinations. Concentrations in the sample were obtained by using the following equations:
(1)Cx=A1ay2-A2ay1ax1ay2-ax2ay1Cy=A1ax2-A2ax1ay1ax2-ay2ax1,
where A1 and A2 are absorbance of mixtures at 240 nm and 270 nm, respectively; ax1 and ax2 are absorptivities of BEN at λ1 and λ2, respectively, ay1 and ay2 are absorptivities of HCT at λ1 and λ2 respectively. Cx and Cy are concentrations of BEN and HCT, respectively.Figure 2
Overlain spectrum of BEN and HCT in Methanol.


### 2.4. Method Validation

The proposed method was validated according to the  International Conference on Harmonization (ICH) guidelines [18].


### 2.5. Linearity and Range

Developed analytical method shows linearity response over the range of 2–12μg/mL for BEN and 4–14 μg/mL for HCT at 240 nm and 270 nm respectively.


### 2.6. Precision

The intraday and interday precision study of the proposed simultaneous equation spectrophotometric method was carried out by estimating responses three times on the same day and on three different days (first, second, and  third days) for three different concentrations of BEN (6, 8, and 10μg/mL) and HCT (8, 10, 12 μg/mL), and the results are reported in terms of percentage relative standard deviation (%RSD).


### 2.7. Accuracy

The accuracy of the method was determined by calculating recoveries of BEN and HCT by the  method of standard additions. Known amount of BEN (50%, 100%, and 150%) and HCT (50%, 100%, and 150%) was added to a pre quantified sample solutions, and the amounts of BEN and HCT were estimated by measuring the  response at the appropriate wavelengths. The recovery was verified by estimation of drugs in triplicate preparations at each specified concentration level.


### 2.8. LOD and LOQ

Calibration curve was repeated 5 times, and the standard deviation (SD) of the intercepts was calculated. Then LOD and LOQ were measured as follows:(2)LOD=3.3×σS,LOQ=10×σS,
where  σ  is the standard deviation of the y-intercept S is the  slope of the calibration curve.


### 2.9. Solution Stability

Solution stability of the method was studied by observing the stability of both drug solutions at25±2°C for 24 h.


### 2.10. Analysis of Marketed Formulation

The pharmaceutical dosage form used in this study was Lotensin HCT tablets with a content of 10 mg BEN and 12.5 mg HCT (as per USP) per tablet. Twenty tablets of brand Lotencin HCT tablets were weighed and finely powdered. Accurately weighed tablet powder equivalent to 10 mg was taken in 100 mL volumetric flask. Few mL of methanol was added and sonicated for 5 min. The volume was made up to the mark with methanol. Aliquot portion of this solution was further diluted to achieve final concentration of 10μg/mL for BEN and HCT. The absorbances were noted at respective wavelengths. The concentration of each drug in tablet formulation was determined using the above methods.


## 2.1. Materials and Methods

A double-beam Shimadzu UV-visible spectrophotometer 1700 (Pharma spec), with wavelength accuracy of ±0.5 nm and a pair of 1 cm matched quartz cells, was used to measure absorbance of the resulting solution. All weighing was done on an electronic balance (Shimadzu BL-220H). All statistical calculations were carried out using Microsoft Excel 2007 analytical tool.Analytically pure BEN and HCT were procured as gift sample from Dishman Pharmaceuticals Ltd. and Cadila Pharmaceutical Pvt. Ltd. (Ahmedabad, India). Methanol (E. Merck, Mumbai, India) analytical grade was used as a diluent. Tablet formulation (Lotensin HCT, Novartis Pharmaceutical Pvt. Ltd.) containing labelled amount of 10 mg of benazepril hydrochloride and 12.5 mg of hydrochlorothiazide was purchased from local market.


## 2.2. Preparation of Solutions

Accurately weighed 100 mg of BEN and HCT standards was transferred to separate 100 mL volumetric flasks and dissolved in 50 mL Methanol. The flasks were shaken, and the volume was made up to the mark with methanol to give solutions containing 1000μg/mL BEN and 1000 μg/mL HCT, respectively.


## 2.3. Methodology

Selection of analytical wavelengths was done by taking pure samples of BEN and HCT, which were separately dissolved in methanol, to give two solutions of 10μg/mL, respectively. They were scanned in the wavelength range of 200–400 nm. From the overlain spectra (Figure 2), wavelengths of  240 and 270 nm were selected for the formation of simultaneous equations. For constructing a calibration curve, two series of different concentrations in range of 2–12 μg/mL for BEN and 4–14 μg/mL for HCT were prepared from stock solutions. The calibration curves were plotted at 240 and 270 nm. The absorptivities (A1%, 1 cm) of both drugs at both wavelengths were determined. These calculated values were the mean of five independent determinations. Concentrations in the sample were obtained by using the following equations:
(1)Cx=A1ay2-A2ay1ax1ay2-ax2ay1Cy=A1ax2-A2ax1ay1ax2-ay2ax1,
where A1 and A2 are absorbance of mixtures at 240 nm and 270 nm, respectively; ax1 and ax2 are absorptivities of BEN at λ1 and λ2, respectively, ay1 and ay2 are absorptivities of HCT at λ1 and λ2 respectively. Cx and Cy are concentrations of BEN and HCT, respectively.Figure 2
Overlain spectrum of BEN and HCT in Methanol.


## 2.4. Method Validation

The proposed method was validated according to the  International Conference on Harmonization (ICH) guidelines [18].


## 2.5. Linearity and Range

Developed analytical method shows linearity response over the range of 2–12μg/mL for BEN and 4–14 μg/mL for HCT at 240 nm and 270 nm respectively.


## 2.6. Precision

The intraday and interday precision study of the proposed simultaneous equation spectrophotometric method was carried out by estimating responses three times on the same day and on three different days (first, second, and  third days) for three different concentrations of BEN (6, 8, and 10μg/mL) and HCT (8, 10, 12 μg/mL), and the results are reported in terms of percentage relative standard deviation (%RSD).


## 2.7. Accuracy

The accuracy of the method was determined by calculating recoveries of BEN and HCT by the  method of standard additions. Known amount of BEN (50%, 100%, and 150%) and HCT (50%, 100%, and 150%) was added to a pre quantified sample solutions, and the amounts of BEN and HCT were estimated by measuring the  response at the appropriate wavelengths. The recovery was verified by estimation of drugs in triplicate preparations at each specified concentration level.


## 2.8. LOD and LOQ

Calibration curve was repeated 5 times, and the standard deviation (SD) of the intercepts was calculated. Then LOD and LOQ were measured as follows:(2)LOD=3.3×σS,LOQ=10×σS,
where  σ  is the standard deviation of the y-intercept S is the  slope of the calibration curve.


## 2.9. Solution Stability

Solution stability of the method was studied by observing the stability of both drug solutions at25±2°C for 24 h.


## 2.10. Analysis of Marketed Formulation

The pharmaceutical dosage form used in this study was Lotensin HCT tablets with a content of 10 mg BEN and 12.5 mg HCT (as per USP) per tablet. Twenty tablets of brand Lotencin HCT tablets were weighed and finely powdered. Accurately weighed tablet powder equivalent to 10 mg was taken in 100 mL volumetric flask. Few mL of methanol was added and sonicated for 5 min. The volume was made up to the mark with methanol. Aliquot portion of this solution was further diluted to achieve final concentration of 10μg/mL for BEN and HCT. The absorbances were noted at respective wavelengths. The concentration of each drug in tablet formulation was determined using the above methods.


## 3. Result and Discussion

A simultaneous equation spectrophotometric method was successfully developed for the  determination of BEN and HCT from their combined dosage form.The proposed simultaneous equation method shows good linearity in the concentration range of 2–12μg/mL for BEN and 4–14 μg/mL for HCT with correlation coefficient 0.9997 of  BEN and 0.9988 for HCT, respectively (Table 2).The %RSD values for BEN and HCT were found to be 1.32% and 1.96%, respectively. The low values of relative standard deviation (less than 2%) indicate that the proposed method is repeatable. The %RSD values for intraday study were found to be 0.47–0.74% and 0.13–0.24% for BEN and HCT, respectively. The %RSD values for interday study were found to be 0.30–0.81% and 0.18–0.40% for BEN and HCT, respectively. The low RSD value indicates that the proposed method is precise (Tables3 and 4). The detection limit of BEN and HCT were 0.103 and 0.025 μg/mL, while quantitation limits of BEN and HCT were 0.312 and 0.075 μg/mL, respectively. The above data shows that a nanogram quantity of both the drugs can be accurately and precisely determined. The validation parameters are summarized in Table 1.Table 1
Summary of validation parameters.
Parameters
BEN
HCT
Limit of detection
0.103
0.025
Limit of quantitation
0.312
0.075
Accuracy (%)
100.0–100.6
99.8–100.2
Precision
Intraday (n=3)
0.47–0.74%
0.13–0.24%
Interday (n=3)
0.30–0.81%
0.18–0.40%
Repeatability (RSD,n=6)
1.32%
1.96%
Specificity
Specific
Specific
Robustness
Robust
Robust
Solvent suitability
Suitable for 24 hrs.
Suitable for 24 hrs.Table 2
Statistical data of BEN and HCT.
Parameters
BEN at 240 nm
HCT at 270 nm
Linear range
2–12 µg/mL
4–14 µg/mL
Slope
0.0181
0.05
Intercept
0.0129
0.035
Standard deviation of intercept
0.0031
0.0010
Standard deviation of slope
0.0005
0.0019
Regression coefficient (R2)
0.9997
0.9988Table 3
Determination of precision for BEN.
BEN
Conc. (μg/mL)
Intraday mean ± SD (n=3)
%RSD
Interday mean ± SD (n=3)
%RSD
At 240 nm
6
0.12267
±
0.00058
0.47
0.124
±
0.001
0.81
8
0.15533
±
0.00115
0.74
0.15533
±
0.00058
0.37
10
0.196
±
0.001
0.51
0.19533
±
0.00058
0.30
At 270 nm
6
0.03067
±
0.00058
1.88
0.03067
±
0.00058
1.88
8
0.3633
±
0.00058
1.59
0.03667
±
0.00058
1.58
10
0.04367
±
0.00058
1.32
0.04767
±
0.00058
1.21Table 4
Determination of precision for HCT.
HCT
Conc. (μg/mL)
Intraday mean ± SD (n=3)
%RSD
Interday mean ± SD (n=3)
%RSD
At 240 nm
8
0.03467
±
0.00058
0.017
0.03433
±
0.00058
1.68
10
0.042
±
0.001
0.024
0.04267
±
0.00058
1.35
12
0.217
±
0.28232
0.18
0.05433
±
0.00058
1.06
At 270 nm
8
0.44333
±
0.00058
0.13
0.44467
±
0.00153
0.34
10
0.526
±
0.001
0.19
0.52567
±
0.00208
0.40
12
0.63367
±
0.00153
0.24
0.63333
±
0.00115
0.18The accuracy of the method was determined by calculating recoveries of BEN and HCT by the method of standard additions. The percent recovery was found to be 100.0%–100.6% for BEN and 99.8%–100.2% for HCT (Table5). The results of recovery studies indicate that the proposed method is accurate.Table 5
Determination of accuracy.
% level
Amount of drug added (μg/mL)
Recovered amount of drug (μg/mL)
% recovery
BEN (μg/mL)
HCT (μg/mL)
BEN (μg/mL)
HCT (μg/mL)
BEN
HCT
50
6
6
6.04
6.00
100.6
100.2
100
8
8
8.01
7.98
100.1
99.89
150
10
10
10.00
9.98
100.0
99.9The proposed validated method was successfully applied to determine BEN and HCT in tablet dosage form. The results obtained for BEN and HCT were comparable with the corresponding labelled amounts (Table6). No interference of the excipients with the absorbance of interest appeared; hence the proposed method is applicable for the routine simultaneous estimation of BEN and HCT in pharmaceutical dosage forms.Table 6
Assay results of marketed formulation.
Formulation
Actual concentration (μg/mL)
Amount obtained (μg/mL)
% BEN
% HCT
BEN
HCT
BEN
HCT
Tablet
4.0
5.0
3.90
4.97
98.25%
99.32%


## 4. Conclusions

Sensitive, precise, and accurate simultaneous UV spectroscopic method was developed and validated. The proposed method is accurate, precise, reproducible, and economic and can be successfully used for routine analysis of simultaneous estimations of BEN and HCT. The method was validated as per the  ICH guidelines in terms of specificity, linearity, accuracy, precision, limits of detection (LOD) and limits of quantification (LOQ), and robustness. The proposed method can be used for quality control assay of BEN and HCT in their pharmaceutical dosage form.



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*Source: 316137-2013-09-16.xml*