# High-Energy X-Ray Microprobe System with Submicron Resolution for X-Ray Fluorescence Analysis of Uranium in Biological Specimens

Authors: Yasuko Terada; Shino Homma-Takeda; Akihisa Takeuchi; Yoshio Suzuki
Journal: X-Ray Optics and Instrumentation
 (2010)

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## Abstract

Total-external-reflection Kirkpatrick-Baez mirror optics for high-energy X-rays have been applied to the X-ray microprobe at beamline 37XU of SPring-8.  A focused beam size of 1.0μm (V)×0.83 μm (H) has been achieved at an X-ray energy of 30 keV, and a total photon flux of the focused beam was about 5×109 photons/s.  Micro-X-ray fluorescence (μ-XRF) analysis of the uranium distribution in rat kidneys has been performed with the mirror-focused beam.  The sensitivity of uranium was evaluated from the XRF intensity of thin standard samples, and the minimum detection limit was estimated at 10 μg/g.  The high-spatial-resolution analysis revealed that uranium was concentrated in the epithelium of the proximal tubules in the inner cortex.  The maximum concentration of uranium in the tubule was estimated to be 503 μg/g using a semiquantitative evaluation.


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## Body

## 1. Introduction

X-ray  microscopy with a total-external-reflection mirror optics is an attractive technique for X-ray fluorescence (XRF) analysis using synchrotron radiation because mirrors are achromatic and give relatively high-brilliance beams compared to other focusing elements.  Suzuki et al. have successfully developed a total-external-reflection mirror system for microfocusing in the X-ray energy range of 30–100 keV at SPring-8 [1].  They have obtained a focused spot of less than 1 μm below X-ray energies of 90 keV using a 200 m long beamline.  Further focusing tests have proved that the mirror system had excellent performance in the high-energy X-ray region and enables not only microscopy but also spectroscopic analysis with sub-micron resolution for high-Z elements.The need for heavy element analysis has been greatly increasing by the importance of heavy metal accumulation in living organisms.  High-energy X-rays (>20 keV) enable direct detection of K lines from high-Z elements.  Nakai et al. have carried out XRF analysis of trace rare-earth and heavy elements using 116 keV X-rays at SPring-8 [2].  They pointed out the advantage of high-energy X-rays for bulk analysis and confirmed that the minimum detection limit (MDL) of the heavy elements was at the sub-ppm level.  More recently, microbeam analysis is required for sub-cellular-level measurements of elemental distributions with high-energy X-rays.  Among the problems of the greatest interest are health effects of uranium on populations. For example, environmental contamination in uranium-polluted areas is continuing to rise and uranium can have serious health impacts, such as renal toxicity.  Total-external-reflection mirror optics developed by Suzuki et al. [1] is one of the most appropriate focusing systems to enable the analysis of trace heavy elements.  In this study, the application of a high-energy scanning X-ray microprobe to evaluate the uranium distribution in rat kidneys is described.


## 2. Experimental

### 2.1. X-Ray Focusing Optics

The basic design of elliptic Kirkpatrick-Baez optics is essentially the same as that described in our previous study [1].  Parabolic mirrors were fabricated by the bent-polished method at Cannon Co. Ltd., Japan [3, 4].  The mirror material was fused quartz with a thickness of 13 mm.  The reflective surface was coated by platinum with a thickness of 1000 Å, and the average glancing angle was 0.8 mrad.  The focal length was 250 mm for the vertical focusing mirror and 100 mm for the horizontal focusing mirror.The experiments were carried out on undulator beamline 37XU of SPring-8 [5].  A schematic diagram of microfocusing optics is illustrated in Figure 1.  X-rays from an undulator were monochromatized by a Si 111 double-crystal monochromator with a rotated-inclined crystal geometry [6].  The first crystal employed a pin-post design to enhance the water cooling efficiency.  The focusing optics was located 57 m from the light source.  The effective length of each mirror was about 80% of full length for focal length of 250 mm and about 65% for focal length of 100 mm, respectively.  It is thought that these effective lengths were limited by the relatively large figure errors at the either end of the mirrors.  The illuminating beam size was determined by an aperture size of an XY slit in front of the mirror (slit size: 64 μm × 52 μm), and only the center area of the mirror was illuminated.Figure 1
Schematic diagram of experimental setup at BL37XU of SPring-8.


### 2.2. Scanning X-Ray Microprobe and Application for Biological Specimen

The K-B microfocusing optics was used for a scanning fluorescence X-ray microprobe system.  Samples were mounted on an X-Y translation stage, and a takeoff angle of 10° was usually used for detecting fluorescence X-rays from the samples.  The fluorescence X-rays were measured by Si(Li) solid state detector which was placed perpendicular to the incident beams to minimize the scattered X-rays.  Figure1 also shows this geometry.Calibration standards with various known concentrations of uranium (20–100μg/g) were used to determine sensitivities and to calibrate intensity levels.  These standards were prepared by mixing standard solutions with a polyvinyl alcohol solution.  The mixed sample solutions were frozen and then were cut into 20 μm sections with a cryomicrotome.  Thin sections of kidneys of adult rats were prepared by using the procedure described in [7].  The rats were exposed to uranium (1.4 mg/kg) by subcutaneous injection.  The animals were treated and handled in accordance with the guidelines of the “Guide for the Care and Use of Laboratory Animals at the National Institute of Radiological Sciences”.  Two days after the administration of uranium, the kidneys were removed and then embedded in an optimal cutting temperature compound to prepare dried sections with 20 μm thickness.  All sections placed on polypropylene film were fixed on an acrylic holder.


## 2.1. X-Ray Focusing Optics

The basic design of elliptic Kirkpatrick-Baez optics is essentially the same as that described in our previous study [1].  Parabolic mirrors were fabricated by the bent-polished method at Cannon Co. Ltd., Japan [3, 4].  The mirror material was fused quartz with a thickness of 13 mm.  The reflective surface was coated by platinum with a thickness of 1000 Å, and the average glancing angle was 0.8 mrad.  The focal length was 250 mm for the vertical focusing mirror and 100 mm for the horizontal focusing mirror.The experiments were carried out on undulator beamline 37XU of SPring-8 [5].  A schematic diagram of microfocusing optics is illustrated in Figure 1.  X-rays from an undulator were monochromatized by a Si 111 double-crystal monochromator with a rotated-inclined crystal geometry [6].  The first crystal employed a pin-post design to enhance the water cooling efficiency.  The focusing optics was located 57 m from the light source.  The effective length of each mirror was about 80% of full length for focal length of 250 mm and about 65% for focal length of 100 mm, respectively.  It is thought that these effective lengths were limited by the relatively large figure errors at the either end of the mirrors.  The illuminating beam size was determined by an aperture size of an XY slit in front of the mirror (slit size: 64 μm × 52 μm), and only the center area of the mirror was illuminated.Figure 1
Schematic diagram of experimental setup at BL37XU of SPring-8.


## 2.2. Scanning X-Ray Microprobe and Application for Biological Specimen

The K-B microfocusing optics was used for a scanning fluorescence X-ray microprobe system.  Samples were mounted on an X-Y translation stage, and a takeoff angle of 10° was usually used for detecting fluorescence X-rays from the samples.  The fluorescence X-rays were measured by Si(Li) solid state detector which was placed perpendicular to the incident beams to minimize the scattered X-rays.  Figure1 also shows this geometry.Calibration standards with various known concentrations of uranium (20–100μg/g) were used to determine sensitivities and to calibrate intensity levels.  These standards were prepared by mixing standard solutions with a polyvinyl alcohol solution.  The mixed sample solutions were frozen and then were cut into 20 μm sections with a cryomicrotome.  Thin sections of kidneys of adult rats were prepared by using the procedure described in [7].  The rats were exposed to uranium (1.4 mg/kg) by subcutaneous injection.  The animals were treated and handled in accordance with the guidelines of the “Guide for the Care and Use of Laboratory Animals at the National Institute of Radiological Sciences”.  Two days after the administration of uranium, the kidneys were removed and then embedded in an optimal cutting temperature compound to prepare dried sections with 20 μm thickness.  All sections placed on polypropylene film were fixed on an acrylic holder.


## 3. Results and Discussion

### 3.1. Performance Test of X-Ray Focusing Elements

The evaluation of focused beam profiles was out with a dark-field knife-edge scan [8] to obtain more accurate beam profiles than with a conventional knife-edge scan.  The intensity of the incident and transmitted X-rays were measured by ionization chambers.  A tantalum blade with a thickness of 3 mm was used as the knife edge.  To satisfy the dark-field condition, a blade placed between focal point and the detector blocks the direct beam.  The detector can feel only the scattered X-rays from the knife edge.  The focused profiles at an X-ray energy of 30 keV are shown in Figure 2.  The focused beam size of 1.0 μm (V) × 0.83 μm (H) full width at half maximum (FWHM) is obtained.  Here, it is useful to consider the theoretical focused beam size to evaluate the experimentally measured beam size.  The actual source size in vertical direction can assume same size of electron beam, which is 15.8 μm.  The horizontal source size is defined by an XY slit placed at 34 m from the light source, and the slit width is set to be 200 μm.  Using the focal length of the mirrors, the distance between the light source and the mirrors, and the actual source size, the geometrical focused beam size is calculated to be 0.096 μm (V) × 0.87 μm (H).  On the other hand, according to the literature [1], the diffraction-limited spot size was 0.13 μm (V) × 0.04 μm (H) at 30 keV.  The measured beam size in the horizontal direction is near the geometrical beam size (0.87 μm).  However, the beam size in the vertical direction is significantly larger than the geometrical beam size (0.096 μm).  This difference is believed to arise from the spread of the beam size in the vertical direction due mainly to the vibration and distortion of the monochrometer crystals.  The total photon flux of the focused beam is about 5 × 109 photons/s at 30 keV.Measured profiles of focused beam at an X-ray energy of 30 keV using dark-field knife-edge scan. (a) Beam profile in the vertical direction (focal length = 250 mm) and (b) beam profile in the horizontal direction (focal length = 100 mm).
(a)(b)


### 3.2. Uranium Distribution of a Rat Kidney

Using the focused beam described in the previous section, the quantitative determination of uranium is considered by a calibration curve technique.  The obtained calibration curve measured at 30 keV X-rays is shown in Figure3.  The data shows good linearity between a uranium concentration of 100 and 20 μg/g.  This technique using thin standards has been discussed and validated as the quantitative analysis with microbeam for biological specimens [9].  The MDL is expressed as(1)MDL=3*(CIN)*(IBt)1/2,
where C is the concentration of uranium (μg/g), IN is the net intensity (number of counts), IB is the background intensity (number of counts) and t is the counting time (10 s) [10].  To quantitatively evaluate the uranium concentration, 5×5 raster scans of the each standards were performed.  For example, relative standard deviation, which is calculated by dividing the standard deviation by the mean value, of the total XRF intensity of uranium was 12% at concentration of 20 μg/g.  According to (1), the estimated MDL value at the lowest end is 10 μg/g.  These results suggest that the system allows us to quantitatively analyze heavy trace elements in biological samples even with microbeam resolution.Figure 3
Calibration curve for the determination of uranium.  The XRF intensity was plotted against the uranium concentration.Prior to theμ-XRF imaging of the samples, XRF imaging with beam size of 200 μm × 200 μm was performed to determine the overall elemental distribution in the renal sections at X-ray energy of 30 keV.  Figure 4 shows the distribution of iron, zinc, and uranium in the kidney.  The intensity of the XRF images is shown as the color scale from blue to white, which corresponds to the XRF intensity from lowest to highest.  Although an XRF image of uranium is usually measured by detecting an ULα line (13.6 keV), here, ULβ lines (Lβ1: 17.2 keV, Lβ2: 16.4 keV) are measured to avoid overlapping by rubidium Kα line (13.4 keV) because of the existence of rubidium in the rat kidney [11].  Iron was localized to the inner stripe of the outer medulla (Figure 4(b)), while the zinc was distributed equally in the cortex and in the outer stripe of the outer medulla than the inner stripe of the outer medulla (Figure 4(c)).   In contrast to the normal elements, uranium shows a completely different distribution.  The uranium was localized only in the outer stripe of the outer medulla (Figure 4(d)).  To get more detailed information about uranium distributions, high resolution μ-XRF imaging in the highly localizing area marked in Figure 4(d) was carried out at the X-ray energy of 37 keV.  The two-dimensional distribution of uranium is shown in Figure 5(a).  The shape of the proximal tubule is obviously visualized by the uranium distribution.  Figure 5(b) represents an XRF spectrum measured at the point indicated by an arrow head in Figure 5(a).  It is observed that the uranium Lβ lines can be clearly detected without interference from other elements.  Uranium concentrations for the image were calculated using the calibration curve showed in Figure 3.  However, a corrected calibration curve was used for estimating the uranium concentration because of different excitation energy between Figure 3 (30 keV) and Figure 5 (37 keV).  Assuming the matrix density and thickness between the samples and standards have approximately the same value, we considered only the correction for the linear absorption coefficients of 30 and 37 keV X-rays.  The model linear absorption coefficients of uranium were 763 cm−1 at 30 keV, and 442 cm−1 at 37 keV, respectively.  These coefficients were provided from the NIST physical reference data [12].  Though this correction is only approximately, it is expected that the calculated values give a semiquantitative map of the uranium concentration.  The maximum concentration of uranium in the tubule was estimated to be 503 μg/g by using the assumptions above.  The quantified value is about 23 times higher than the mean concentration in the kidney (22.3 μg/g wet weight at 2 days measured by inductively-coupled plasma mass spectrometry).  This intense localization is in good agreement with a previous study [7].  The elemental imaging indicated that uranium is selectively distributed in the proximal tubules and highly concentrated in the epithelium of the tubules.XRF imaging of a renal cross section.  (a) photograph of a serial section showing the imaging area.  The two-dimensional distribution of (b) iron, (c) zinc, and (d) uranium.  Beam size: 200μm (V) × 200 μm (H), imaging area: 4.5 mm (V) × 4 mm (H), pixel size: 100 μm, and dwell time: 5 s.
(a)(b)(c)(d)High resolutionμ-XRF image of the marked area in Figure 4(d) measured at 37 keV X-rays.  (a) The two-dimensional distribution of uranium.  The XRF intensities are estimated to the concentration of uranium.  Beam size: 1.2 μm (V) × 1.4 μm (H), imaging area: 75 μm (V) × 57 μm (H), pixel size: 0.5 μm, and dwell time: 5 s.  (b) X-ray fluorescence spectrum at arrow head in Figure 5(a).  Measurement time is 600 s.
(a)(b)


## 3.1. Performance Test of X-Ray Focusing Elements

The evaluation of focused beam profiles was out with a dark-field knife-edge scan [8] to obtain more accurate beam profiles than with a conventional knife-edge scan.  The intensity of the incident and transmitted X-rays were measured by ionization chambers.  A tantalum blade with a thickness of 3 mm was used as the knife edge.  To satisfy the dark-field condition, a blade placed between focal point and the detector blocks the direct beam.  The detector can feel only the scattered X-rays from the knife edge.  The focused profiles at an X-ray energy of 30 keV are shown in Figure 2.  The focused beam size of 1.0 μm (V) × 0.83 μm (H) full width at half maximum (FWHM) is obtained.  Here, it is useful to consider the theoretical focused beam size to evaluate the experimentally measured beam size.  The actual source size in vertical direction can assume same size of electron beam, which is 15.8 μm.  The horizontal source size is defined by an XY slit placed at 34 m from the light source, and the slit width is set to be 200 μm.  Using the focal length of the mirrors, the distance between the light source and the mirrors, and the actual source size, the geometrical focused beam size is calculated to be 0.096 μm (V) × 0.87 μm (H).  On the other hand, according to the literature [1], the diffraction-limited spot size was 0.13 μm (V) × 0.04 μm (H) at 30 keV.  The measured beam size in the horizontal direction is near the geometrical beam size (0.87 μm).  However, the beam size in the vertical direction is significantly larger than the geometrical beam size (0.096 μm).  This difference is believed to arise from the spread of the beam size in the vertical direction due mainly to the vibration and distortion of the monochrometer crystals.  The total photon flux of the focused beam is about 5 × 109 photons/s at 30 keV.Measured profiles of focused beam at an X-ray energy of 30 keV using dark-field knife-edge scan. (a) Beam profile in the vertical direction (focal length = 250 mm) and (b) beam profile in the horizontal direction (focal length = 100 mm).
(a)(b)


## 3.2. Uranium Distribution of a Rat Kidney

Using the focused beam described in the previous section, the quantitative determination of uranium is considered by a calibration curve technique.  The obtained calibration curve measured at 30 keV X-rays is shown in Figure3.  The data shows good linearity between a uranium concentration of 100 and 20 μg/g.  This technique using thin standards has been discussed and validated as the quantitative analysis with microbeam for biological specimens [9].  The MDL is expressed as(1)MDL=3*(CIN)*(IBt)1/2,
where C is the concentration of uranium (μg/g), IN is the net intensity (number of counts), IB is the background intensity (number of counts) and t is the counting time (10 s) [10].  To quantitatively evaluate the uranium concentration, 5×5 raster scans of the each standards were performed.  For example, relative standard deviation, which is calculated by dividing the standard deviation by the mean value, of the total XRF intensity of uranium was 12% at concentration of 20 μg/g.  According to (1), the estimated MDL value at the lowest end is 10 μg/g.  These results suggest that the system allows us to quantitatively analyze heavy trace elements in biological samples even with microbeam resolution.Figure 3
Calibration curve for the determination of uranium.  The XRF intensity was plotted against the uranium concentration.Prior to theμ-XRF imaging of the samples, XRF imaging with beam size of 200 μm × 200 μm was performed to determine the overall elemental distribution in the renal sections at X-ray energy of 30 keV.  Figure 4 shows the distribution of iron, zinc, and uranium in the kidney.  The intensity of the XRF images is shown as the color scale from blue to white, which corresponds to the XRF intensity from lowest to highest.  Although an XRF image of uranium is usually measured by detecting an ULα line (13.6 keV), here, ULβ lines (Lβ1: 17.2 keV, Lβ2: 16.4 keV) are measured to avoid overlapping by rubidium Kα line (13.4 keV) because of the existence of rubidium in the rat kidney [11].  Iron was localized to the inner stripe of the outer medulla (Figure 4(b)), while the zinc was distributed equally in the cortex and in the outer stripe of the outer medulla than the inner stripe of the outer medulla (Figure 4(c)).   In contrast to the normal elements, uranium shows a completely different distribution.  The uranium was localized only in the outer stripe of the outer medulla (Figure 4(d)).  To get more detailed information about uranium distributions, high resolution μ-XRF imaging in the highly localizing area marked in Figure 4(d) was carried out at the X-ray energy of 37 keV.  The two-dimensional distribution of uranium is shown in Figure 5(a).  The shape of the proximal tubule is obviously visualized by the uranium distribution.  Figure 5(b) represents an XRF spectrum measured at the point indicated by an arrow head in Figure 5(a).  It is observed that the uranium Lβ lines can be clearly detected without interference from other elements.  Uranium concentrations for the image were calculated using the calibration curve showed in Figure 3.  However, a corrected calibration curve was used for estimating the uranium concentration because of different excitation energy between Figure 3 (30 keV) and Figure 5 (37 keV).  Assuming the matrix density and thickness between the samples and standards have approximately the same value, we considered only the correction for the linear absorption coefficients of 30 and 37 keV X-rays.  The model linear absorption coefficients of uranium were 763 cm−1 at 30 keV, and 442 cm−1 at 37 keV, respectively.  These coefficients were provided from the NIST physical reference data [12].  Though this correction is only approximately, it is expected that the calculated values give a semiquantitative map of the uranium concentration.  The maximum concentration of uranium in the tubule was estimated to be 503 μg/g by using the assumptions above.  The quantified value is about 23 times higher than the mean concentration in the kidney (22.3 μg/g wet weight at 2 days measured by inductively-coupled plasma mass spectrometry).  This intense localization is in good agreement with a previous study [7].  The elemental imaging indicated that uranium is selectively distributed in the proximal tubules and highly concentrated in the epithelium of the tubules.XRF imaging of a renal cross section.  (a) photograph of a serial section showing the imaging area.  The two-dimensional distribution of (b) iron, (c) zinc, and (d) uranium.  Beam size: 200μm (V) × 200 μm (H), imaging area: 4.5 mm (V) × 4 mm (H), pixel size: 100 μm, and dwell time: 5 s.
(a)(b)(c)(d)High resolutionμ-XRF image of the marked area in Figure 4(d) measured at 37 keV X-rays.  (a) The two-dimensional distribution of uranium.  The XRF intensities are estimated to the concentration of uranium.  Beam size: 1.2 μm (V) × 1.4 μm (H), imaging area: 75 μm (V) × 57 μm (H), pixel size: 0.5 μm, and dwell time: 5 s.  (b) X-ray fluorescence spectrum at arrow head in Figure 5(a).  Measurement time is 600 s.
(a)(b)


## 4. Conclusion

A scanning fluorescence X-ray microprobe with sub-micron transverse spatial resolution was achieved at beamline 37XU of SPring-8 using total-external-reflection K-B mirror optics.  A focused beam size of 1.0μm (V) × 0.83 μm (H) FWHM at the X-ray energy of 30 keV was achieved.  The mirror optics provides sufficient spatial resolution to examine μ-XRF analysis of high-Z elements even with high-energy X-rays.  The quantitative evaluation of μ-XRF analysis was performed by a calibration-curve method.  The MDL with a beam size of around 1 μm2 was 10 μg/g.  The X-ray microprobe was also utilized to map the uranium distribution in the kidneys of rats exposed to uranium acetate.  It was revealed that uranium localized in the proximal tubule with a 23 times higher concentration compared to the whole kidney.  These results provide useful information to understanding the chemical and radiological toxicity of uranium in the kidney.  This technique will contribute greatly to understand dynamic mechanism of trace high-Z elements with sub-micron resolution and will open new application for a variety of fields using XRF analysis.



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*Source: 317909-2010-07-05.xml*