# Nutraceutical Profiling, Bioactive Composition, and Biological Applications ofLepidium sativum L.

Authors: Sakshi Painuli; Cristina Quispe; Jesús Herrera-Bravo; Prabhakar Semwal; Miquel Martorell; Zainab M. Almarhoon; Ainur Seilkhan; Alibek Ydyrys; Javad Sharifi Rad; Mohammed M. Alshehri; Sevgi Durna Daştan; Yasaman Taheri; Daniela Calina; William C. Cho
Journal: Oxidative Medicine and Cellular Longevity
 (2022)

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## Abstract

The roots, leaves, and seeds ofLepidium sativum L., popularly known as Garden cress in different regions, have high economic importance; although, the crop is particularly cultivated for the seeds. In traditional medicine, this plant has been reported to possess various biological activities. This review is aimed at providing updated and critical scientific information about the traditional, nutritional, phytochemical, and biological activities of L. sativum. In addition, the geographic distribution is also reviewed. The comprehensive literature search was carried out with the help of different search engines PubMed, Web of Science, and Science Direct. This review highlighted the importance of L. sativum as an edible herb that possesses a wide range of therapeutic properties along with high nutritional values. Preclinical studies (in vitro and in vivo) displayed anticancer, hepatoprotective, antidiabetic, hypoglycemic, antioxidant, antimicrobial, gastrointestinal, and fracture/bone healing activities of L. sativum and support the clinical importance of plant-derived bioactive compounds for the treatment of different diseases. Screening of literature revealed that L. sativum species and their bioactive compounds may be a significant source for new drug compounds and also could be used against malnutrition. Further clinical trials are needed to effectively assess the actual potential of the species and its bioactive compounds.


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## Body

## 1. Introduction

A large number of people or community from developed and developing countries depend on medicinal plants for treatments, skin care, cultural progress, and economic growth [1, 2]. The World Health Organization (WHO) projected that 80% of the world’s population relies on traditional medicines, and around 19.4 billion global revenue were recorded for herbal remedies in 2010 [3, 4]. The market demand for medicinal plants is increasing continuously and according to WHO the demand will be more than the US $ 5 trillion in 2050 [5].Lepidium sativum L. popularly known as garden cress in different regions of the world is an edible annual and fast-growing herb belongs to the family Brassicaceae and genus Lepidium [6]. The genus consists of more than 175 species around the world; among them, several species are known for their nutritional and therapeutic properties [7, 8].From prehistoric time,L. sativum has been consumed by ancient Egyptians and Romans for several health-promoting benefits [9]. Traditionally, L. sativum is used for the treatment of various diseases like asthma, tumors of the uterus, ulcers, hemorrhoidal haemorrhage, coughing, wounds, dermatomycosis, dysmenorrhea, sciatica, and nasal polyps. The seeds of this species have been utilized as a galactagogues and abortive agent and are also used to treat sore throat, headache, cough, asthma, malaria, syphilis, and impotence [10]. A seed paste prepared in water is used for skin problems and sunburns while the mucus of seeds is used against diarrhoea and irritation of the intestines in dysentery, and germinating seeds are used for constipation [11, 12]. The leaves of L. sativum are diuretic, mildly stimulant, and also used in liver problems and scorbutic diseases [13].People consume it in the form of salad, sprouts, and spicy seasoning, and the oil extracted from their seed is used for seasoning [14, 15]. Different parts such as roots, leaves, and seeds of this plant species have immense economic importance; although, the crop is particularly cultivated for the seeds [16].L. sativum has been reported to possess various biological activities such as antimicrobial, bronchodilator, hypotensive, allopathic, hypoglycemic, hepatoprotective, antioxidant, and against hiccup [17–21]. Its mucilage possesses various characteristics such as gelling, binding, and disintegrating, which help in the development of desirable pharmaceutical dosage forms [16]. The phytochemical profiling of L. sativum showed the presence of flavonoids, phenols, cardiotonic glycosides, cardiac glycosides, alkaloids, coumarins, proteins, and amino acids [10].The purpose of the present review is to provide updated and quantified scientific information about the traditional, nutritional, phytochemical, and biological activities ofL. sativum.


## 2. Methodology

For this review, we collected literature published in English from scientific databases such as PubMed, Web of Science, and ScienceDirect, before July 2021 on phytochemistry, nutritional profile, and pharmacology of Lepidium sativum species. The following MESH terms were used for searching: “Lepidium sativum/chemistry,” Seeds/chemistry, Oxidative Stress/drug effects, “Plant Extracts/chemistry,” “Plant Extracts/pharmacology,” “Antineoplastic Agents,” “Antioxidants/chemistry,” “Antioxidants/pharmacology,” “Apoptosis/drug effects,” “Cell Line, Tumor,” “DNA Damage/drug effects,” “Flavonoids/chemistry,” “Flavonoids/pharmacology,” “Blood Glucose,” “Diabetes Mellitus,” “Experimental/drug therapy,” “Hyperglycemia/drug therapy,” “Hypoglycemic Agents/pharmacology,” “Animals,” and “Humans.”All selected papers were analyzed and summarized to prepare this comprehensive review.The plant taxonomy was verified by the database “The PlantList,” and the chemical formulas were validated with Chemspider [22, 23].


## 3. Bioactive Compounds

Active compounds or secondary metabolites are produced in plants as a byproduct of various metabolic reactions; although they do not play a primary role in plant reactions, they are important in many plant defence mechanisms and are also known for their biological or therapeutic activities [24–26]. The most important class of secondary metabolites are phenols, flavonoids, terpenoids, alkaloids, saponins, and glycosides [27–29].Phytochemically, the seeds, leaves, roots, and seed oil ofL. sativum are a rich source of alkaloids, glucosinolates, saponins, terpenes, saturated, and essential fatty acids [13, 30–33].Glucosinolates are a wide group of secondary metabolites consisting of sulphur and nitrogen molecules and are mainly known for their nutritional effects and other therapeutic properties like antimicrobial, antioxidant, anticancer, and anti-inflammatory [34, 35].Total phenolic and flavonoid content ofL. sativum leaves of two cultivars (Dadas and Izmir from Turkey) was measured to be 0.573 mg gallic acid equivalent (GAE)/g fresh weight (FW) and 6.332 mg GAE/g DW for Dadas cultivar and 0.774 mg GAE/g FW and 7.401 mg GAE/g DW for Izmir cultivar, respectively [36]. The ascorbic acid content for L. sativum leaves was measured to be 54 mg/100 g FW and 74 mg/100 g FW for Dadas and Izmir cultivars [36]. However, the methanolic extract of seeds showed the presence of 0.5% and 0.375% of phenolic and flavonoid content [32].Malar et al. [37] reported the ascorbic acid content in stem (11.74±0.83mg), leaves (7.4±0.38mg), whole plant (12.5±0.60mg), and seeds (9.68±0.72mg) of L. sativum.Chatoui et al. [38] showed the presence of tannin in the ethanolic and methanolic seed extract of L. sativum collected from different regions of Morocco. The maximum tannin acid (31.50±0.11 mg catechin/g extract) was observed in methanolic seed extract of L. sativum of Ben-Ahmed region, Morocco, whereas the minimum (8.33±0.11 mg catechin/g extract) amount of tannin was measured in the ethanolic extract of L. sativum of Rommani region, Morocco [38]. Other studies from different regions also showed that L. sativum has a significant amount of phenolic and flavonoid content (Table 1).Table 1
Total phenolic and flavonoid content inLepidium sativum.
Country
Plant part and solvents
Total phenolic content (mg gallic acid equivalent/g extract)
Total flavonoid content (mg quercetin equivalent/g extract)
Ref.
India
Ethanolic seed extract
4.46
±
0.14
3.57
±
1.2
[39]
Ethanolic seed extract
11.03
±
0.75
4.79
±
0.24
[40]
Pakistan
Methanolic seed extract
120.26
±
1.52
∗
—
[41]
Egypt
Aqueous seed extract
126.24
007.21
[42]
Ethanolic seed extract
88.08
00.65
[42]
Ethanolic seed extract
46.00
±
0.86
82.00
±
0.93
[43]
Aqueous seed extract
34.00
±
0.67
53.00
±
0.58
[43]
Turkey
Methanolic extract of aerial part
184.14
±
2.5
∗
∗
12.63
±
1.5
∗
∗
∗
[44]
Morocco
Tafraout region
Methanolic seed extract
94.48
±
1.82
37.63
±
2.14
[38]
Ethanolic seed extract
86.48
±
0.22
32.51
±
0.81
[38]
El-Haouz region
Methanolic seed extract
83.36
±
0.98
33.58
±
0.33
[38]
Ethanolic seed extract
80.28
±
0.28
29.24
±
0.47
[38]
Ben-Ahmed region
Methanolic seed extract
69.46
±
0.09
24.85
±
0.48
[38]
Ethanolic seed extract
65.15
±
1.07
23.92
±
0.64
[38]
Rommani region
Methanolic seed extract
59.40
±
0.62
21.09
±
0.21
[38]
Ethanolic seed extract
52.79
±
0.30
20.04
±
0.04
[38]
∗mg catechin equivalent/g extract; ∗∗μg gallic acid equivalent/mg extract; ∗∗∗μg quercetin equivalent/mg extract.Regarding the essential oil composition, Afsharypuor and Hadi [45] identified the presence of 1,8-cineole, benzyl isothiocyanate, α-pinene, and phenyl acetonitrile in seeds, benzyl isothiocyanate, α-pinene, palmitic acid, and linoleic acid in roots, and benzyl isothiocyanate, α-pinene, palmitic acid, phenyl acetonitrile, sabinene, and limonene, β-thujone in the aerial part of L. sativum by gas chromatography-mass spectrometry (GC-MS) analysis [45].The seeds ofL. sativum are comprised of 24% oil which contains linoleic acid and α-linoleic acid. It is reactively more stable due to the presence of phytosterols and antioxidant content [46, 47].Singh et al. [48] reported the presence of 2-pentanoic acid, penta-decadienoic acid, pentanoic acid, succinic acid, butyric acid, acetic acid, oxalic acid, carbonic acid, propanoic acid, and cyclohexane carboxylic acid in the seed oil of L. sativum. The chemical structures of bioactive compounds present in the essential oil of the species are shown in Figures 1(a) and 1(b) while a detail description of essential oil composition has been presented in Table 2.Figure 1
(a) Chemical structure of several bioactive compounds present in essential oil ofL. sativum. (b) Chemical structure of several bioactive compounds present in the essential oil of L. sativum.
(a)
(b)Table 2
The chemical composition of essential oils isolated from different parts ofL. sativum.
Plant part used
Bioactive compounds
Regions/ country
References
Aerial part
Alpha-pinene; sabinene; limonene; 1,8-cineole;β-thujone; phenylacetonitrile; benzyl isothiocyanate; hexadecanoic acid; linoleic acid.
Iran
[45]
Seeds
Alpha-pinene; 1,8-cineole; phenylacetonitrile; benzyl isothiocyanate.
Iran
[45]
Alpha-pinene; sabinene; alpha-phellandrene; eucalyptol; gamma-terpinene; linanool; terpinen-4-ol; alpha-terpineol; propanoate; alpha-terpinyl acetate; E-nerolidol.
Greece
[49]
Docosatrienoic acid; linoleic acid; eicosenoic acid; palmitic acid; arachidonoic acid; eruic acid; stearic acid; arachidic acid.
Ethiopia
[50]
Alpha-tocopherol; gamma-tocopherol; cholesterol; campesterol; stigmasterol; sitosterol; avenasterol.
USA
[47]
7,10-Hexadecadienoic acid, methyl ester; 11-octadecenoic acid, methyl ester; behenic acid, methyl ester; 7,10,13-hexadecatrienoic acid, methyl ester; stearic acid, methyl ester; hexadecanoic acid, 15-methyl-, methyl ester; 15-tetracosenoic acid, methyl ester; 10-octadecenoic acid, methyl ester; heneicosanoic acid, methyl ester.
Saudi Arabia
[51]
Myristic acid; palmitic acid; palmitoleic acid; stearic acid; oleic acid; linoleic acid; linolenic acid; arachidic acid; gadoleic acid; cholesterol acid; stigmasterol; campesterol; beta-sitosterol; 5-avenasterol; alpha-tocopherol; beta-tocopherol; gamma-tocopherol.
Morocco
[38]
Toluene, octane, (E,Z)-1,3,5-octatriene, ethylbenzene, 2-furanmethanol, styrene, methional, benzaldehyde, benzyl alcohol, benzaldehyde, benzyl alcohol, 1-isocyano-2-methylbenzene, benzyl isothiocyanate, benzylurea, 1-benzyl-2(1 H)-pyridone, (Z)-8-heptadecene, palmitic acid, cyclic octaatomic sulfur, oleic acid, linoleic acid, nonadecanamide, arachidic acid, etc.
India
[20]
Linolenic acid, oleic acid, arachidic acid, palmitic acid, stearic acid.
India
[52]
Myristic acid; palmitoleic acid; palmitic acid; alpha-linolenic acid; octadecenoic acid; stearic acid; 9-octadecen-12-ynoic acid; paullinic acid; arachidic acid; erucic acid; behenic acid; nervonic acid; lignoceric acid.
Saudi Arabia
[53]
Alpha-linolenic acid; oleic acid; linoleic acid; eicosanoic acid; palmitic acid; erucic acid; arachidic acid; stearic acids.
India
[46]
Beta-amyrin; 9,12,15-octadecatrienoic acid methyl ester; 9-octadecenoic acid methyl ester; alpha-amyrin; 11-eicosenoic acid methyl ester; 9,12-octadecadienoic acid; hexadecanoic acid methyl ester.
Saudi Arabia
[54]
Geraniol; citronellol; nerol; triacontane; palmitic acid; 1,6-octadien-3-ol, 3,7-dimethyl.
Egypt
[55]
Roots
Alpha-pinene; benzyl isothiocyanate; hexadecanoic acid; linoleic acid.
Iran
[45]The analyses of the chemical composition ofL. sativum extract revealed the presence of five glucosinolates in seeds (glucotropaeolin and 2-phenyl ethyl glucosinolate) and fresh herb (glucotropaeolin, methyl glucosinolate, 2-ethyl butyl glucosinolate, and butyl glucosinolate) [56]. Williams et al. (2009) reported the presence of glucotropaeolin as a principal glucosinolate and gluconasturtiin in the seeds of L. sativum [57]. Hussain et al. (2011) [58] reported the presence of 19 phytochemicals in the methanolic leaves to extract L. sativum including campesterol, cis-vaccenic acid, 2-naphthalenol, 1-nitro-2-propanol,1-deoxy-d-mannitol, allyl isothiocyanate, and paromomycin, among others.Maier et al. [59] identified the imidazole alkaloid lepidine along with five new dimeric (lepidines B, C, D, E, and F) and two monomeric (semilepidinosides A and B) imidazole alkaloids in seeds of L. sativum [59], while the presence of 10 major compounds includes benzyl nitrile, 2,3,4-tri-methoxycinna-mic acid, 5-hydroxy-methyl furfural, and furfural was reported by El-Gendy [60].A complete screening of phytochemicals present inL. sativum seeds was evaluated by ultrahigh-performance liquid chromatography (UHPLC)/photodiode array detection (PDA)/electrospray ionization-mass spectroscopy (ESI-MS) method as well as head space solid-phase microextraction (SPME)-GC/MS methods [61]. A total of 32 metabolites from flavonoid, glucosinolate, phenolic acid, sugar, coumarin, lignan, glycoalkaloid, steroid, and fatty acid classes were identified via UHPLC/PDA/ESI-MS, and 66 metabolites from alcohol, acid, ester, aromatic, ketone, aldehyde, monoterpene hydrocarbon, and among other classes were identified by (SPME)-GC/MS [61]. All the above studies are reported from different regions including Saudi Arabia, India, Egypt, and Iraq, which indicates that the leaves, seed, or seed oil of L. sativum could be a valuable source of important active compounds with significant biological activity.The chemical structure of bioactive compounds present in the extracts ofL. sativum has been displayed in Figure 2 while a detailed description of bioactive compounds present in different parts of the species has been presented in Table 3.Figure 2
Chemical structure of bioactive compounds present inL. sativum extracts.Table 3
The chemical composition ofLepidium sativum extracts.
Plant part used
Bioactive compounds
Regions/country
References
Leaves
Benzyl nitrilen,n-Dimethylaminoethanol2-Hydroxy-1-(1′-pyrrolidiyl)-1-buten-3-oned-ProlineButyrolactone
Iraq
[62]
ApigeninQuercetinKaempferolLuteolin7-Hydroxy-4′,5,6-trimethoxyisoflavone;Sinapic acidChlorogenic acidp-coumaric acidAscorbic acidα-Tocopherol6-prenylnaringenin.
Egypt
[63]
Seeds
Glucotropaeolin; sinapineK di-hexose rhamnoseSinapoyl di-glucose; sinapoyl malateK hexose rhamnose 1K rhamnose (benzo) di-hexose 1
Algeria
[64]
Benzyl nitrileBenzene-isothiocyanatomethyl3′,5′-dimethoxyacetophenoneHexadecanoic acid methyl estercis-Vaccenic acidcs-11-Eicosenoic acid-methyl ester7,8-Epoxylanostan-11-ol, 3-acetoxyeergosta-14,22-dien-3-ol- acetate -3 beta-5 alpha
India
[65]
Benzyl cyanideBenzyl thiocyanateBenzyl isothiocyanateBenzaldehydeBenzonitrileBenzyl thiocyanateBenzyl isothiocyanate
Poland
[66]
Aerial part
Stigmast-5-en-3β27-Diol 27-benzoate
India
[67]


## 4. Nutritional Profile

L. sativum is considered a valuable source of nutrition with significant therapeutic properties. In the last few years, several researchers from different regions have investigated the nutritional profiling of the leaves, seed, and seed oil of L. sativum (Tables 4 and 5).Table 4
Nutritional composition of leaves ofLepidium sativum.
(a)
Proximate composition
Component
Nigeria (g/100 gDW±SD) [68]
Bangladesh (g/100 gDW±SD) [71]
Nigeria (%) [72]
Moisture
91.05
±
1.41
87.13
±
0.088
81.85
Ash
15.38
±
0.21
1.80
±
0.015
3.25
Crude fiber
9.31
±
0.13
2.38
±
0.015
8.69
Crude protein
18.25
±
0.1
2.53
±
0.041
1.01
Total carbohydrate
55.34
±
0.20
5.47
±
0.025
5.82
Total lipid
1.72
±
0.18
—
8.08
Total fat
—
0.70
±
0.029
—
(b)
Minerals
Principal component
Nigeria (mg/100 gDW±SD) [68]
Potassium
1850.00
±
43.30
Phosphorus
4.10
±
0.44
Magnesium
160.60
±
6.56
Calcium
829.13
±
20.70
Iron
63.47
±
5.27
Sodium
141.13
±
38.19
Copper
0.39
±
0.02
Chromium
0.36
±
0.27
Zinc
2.28
±
0.07
Manganese
5.74
±
0.11
(c)
Aminoacids
Principal component
Nigeria (g/100 g proteinDW±SD) [68]
Isoleucine (Ile)∗
3.26
±
1.05
Leucine (Leu)∗
6.84
±
1.02
Lysine (Lys)∗
3.5
±
0.21
Methionine (Met)∗
1.11
±
0.1
Cysteine (Cys)
0.42
±
0.21
Phenylalanine (Phe)∗
4.77
±
2.02
Tyrosine (Tyr)
2.59
±
1.20
Threonine (Thr)∗
2.61
±
1.04
Valine (Val)∗
3.85
±
0.25
Alanine (Ala)
4.31
±
0.90
Arginine (Arg)∗
4.32
±
1.78
Aspartic acid (Asp)
7.73
±
2.77
Glutamic acid (Glu)
9.36
±
0.06
Glycine (Gly)
1.24
±
0.24
Histidine (His)∗
2.09
±
1.00
Proline (Pro)
2.16
±
0.16
Serine (Ser)
2.31
±
0.01
∗Essential amino acids. DW: dry weight; SD: standard deviation.Table 5
Nutritional composition of seed ofLepidium sativum.
(a)
Proximate content
Component
India (g/100 g) [70]
Indian (g/100 g DW) [73]
Saudi Arabia (%) [69]
Pakistan (%) [41]
Egypt (%) [74]
Moisture
4.14
±
0.05
4.82
±
0.09
4.89
±
0.050
3.92
±
1.06
7.05
±
0.45
Ash
4.65
±
0.09
4.95
±
0.00
5.83
±
0.389
4.25
±
0.13
4.8
±
0.88
Crude fiber
7.01
±
0.08
9.72
±
0.32
6.80
±
0.080
6.75
±
1.02
18.79
±
0.79
Crude protein
22.47
±
0.78
26.31
±
0.03
19.82
±
0.205
24.18
±
1.5
19.73
±
1.03
Total carbohydrate
34.24
±
0.92
29.25
±
0.27
34.24
±
0.092
32.87
±
0.29
35.45
±
1.65
Total lipid
—
—
—
28.03
±
1.05
—
Total fat
27.48
±
0.14
24.96
±
0.02
—
—
14.18
±
0.94
(b)
Mineral composition
Principal component
India (mg/100g±SD) [70]
Saudi Arabia (mg/100g±SD) [69]
Pakistan (mg/100gofseed±SD) [41]
Potassium
1193.95
±
10.51
785.0
±
7.51
1236.51
±
1.67
Phosphorus
514.59
±
10.67
616.50
±
9.67
608.63
±
1.39
Magnesium
315.25
±
3.63
—
339.23
±
2.13
Calcium
296.60
±
1.04
253.0
±
1.04
266.35
±
1.44
Iron
7.62
±
0.04
53.81
±
0.04
8.31
±
0.36
Sodium
24.64
±
0.02
—
19.65
±
0.98
Copper
5.53
±
0.09
1.90
±
0.09
5.73
±
2.11
Zinc
5.05
±
0.07
4.10
±
0.07
6.99
±
0.54
Manganese
2.57
±
0.04
—
2.00
±
1.08
Sulphur
293.02
±
14.27
—
—
Aluminum
2.82
±
0.13
—
—
Boron
1.41
±
0.03
—
—
Molybdenum
0.43
±
0.08
—
—
(c)
Fatty acid profile
Fatty acid
India (%) [70]
Saudi Arabia (%) [69]
Pakistan
%
g
/
100
g
of
L
.
s
a
t
i
v
u
m
±
SD [41]
Palmitic acid
8.7
8.80
10.30
±
0.12
Oleic acid
19.9
23.49
30.50
±
0.16
Palmitoleic acid
—
—
0.70
±
0.30
Stearic acid
3.2
3.49
1.90
±
0.19
Myristic acid
1.9
1.50
—
Linolenic acid
12.1
30.07
—
Linoleic acid
30.2
11.35
8.60
±
0.38
Eicosenoic acid
10.3
12.60
—
Erucic acid
—
4.64
—
Arachidic acid
3.2
4.06
—
(d)
Amino acid composition
Principal component
Saudi Arabia (g/100 gprotein±SD) [69]
Pakistan (g/100 gprotein±SD) [70]
Isoleucine (Ile)∗
5.21
±
0.014
5.11
±
0.03
Leucine (Leu)∗
9.03
±
0.007
8.21
±
0.01
Lysine (Lys)∗
2.26
±
0.390
6.26
±
0.39
Methionine (Met)∗
1.86
±
0.000
0.97
±
0.02
Cysteine (Cys)
0.80
±
0.000
—
Phenylalanine (Phe)∗
5.80
±
0.004
5.65
±
0.03
Tyrosine (Tyr)
3.82
±
0.000
2.69
±
0.09
Threonine (Thr)∗
5.39
±
0.019
2.66
±
0.09
Valine (Val)∗
6.24
±
0.007
8.04
±
0.03
Alanine (Ala)
—
4.83
±
0.02
Arginine (Arg)∗
—
4.51
±
0.03
Aspartic acid (Asp)
—
9.76
±
0.03
Glutamic acid (Glu)
—
19.33
±
0.19
Glycine (Gly)
—
5.51
±
0.07
Histidine (His)∗
3.51
±
0.007
3.87
±
0.14
Proline (Pro)
—
5.84
±
0.38
Serine (Ser)
—
4.96
±
0.09
Phenylalanine + tyrosine (Phe + Tyr)
9.62
±
0.000
—
Methionine + cysteine (Met + Cys)
1.86
±
0.000
—
∗Essential amino acids. DW: dry weight; SD: standard deviation.Hassan et al. [68] evaluated that in L. sativum leaves, the highest amount of mineral value was observed for potassium (1850.00±43.30 mg/100 g dry weight (DW)) followed by calcium (829.13±20.70 mg/100 g DW), and the minimum value was observed for chromium (0.36±0.27 mg/100 g DW); however, the maximum amino acid content in leaves was calculated for glutamic acid (9.36±0.06 g/100 g protein DW), and minimum value was shown by cysteine (0.42±0.20g/100 g protein DW) [58].In three studies from different regions (Nigeria, Saudi Arabia, and Pakistan), the highest mineral value ofL. sativum seed was calculated for potassium (1193.95±10.51; 785.0±7.51; 1236.5±1.67 mg/100 g) followed by phosphorus (514.59±10.67; 616.50±9.67; 608.63±1.39 mg/100 g) [41, 69, 70], and the minimum mineral value was observed for molybdenum (0.43±0.08 mg/100 g) [70].The amino acid analyses showed different results in terms of the maximum and minimum amino acid value, and it was recorded for glutamic acid (19.33±0.19g/100 g protein) and methionine (0.97±0.02g/100 g protein) [70]; however, in another study, the highest amino acid value was measured for leucine (9.03±0.007g/100 g protein), and lowest amino acid values were measured for cysteine (0.80±0.00g/100 g protein) [69].The estimation of fatty acid was done for three seed oil extracts ofL. sativum prepared from the cold press extraction method, Soxhlet extraction method, and supercritical carbon dioxide extraction method. The study findings showed that in all the seed oil extracts, the maximum fatty acid content was measured for linoleic acid (~34-35%), and the minimum was observed in oleic acid (~2.8%) [46].The nutritional profiling showed that the leaves, seeds, and seed oil ofL. sativum possess appropriate nutritional content which can help in combating anemia, malnutrition, and several micronutrient deficiencies (Figure 3).Figure 3
The most representative nutritional compounds ofLepidum sativum and the correlation with their beneficial effects for human health.


## 5. Pharmacological Properties

The major role of food is to fulfil the requirement of necessary nutrients in the body and to satisfy hunger; however, nowadays, food from edible plants also plays a significant role in preventing and curing several diseases and disorders due to the presence of different bioactive compounds [75]. The species comprise a variety of bioactive compounds along with strong nutraceutical potential and showed several biological activities [76]. In this section, we discussed different biological applications of the species including anticancer, hepatoprotective, antidiabetic and hypoglycemic, antioxidant, antimicrobial, gastrointestinal, and fracture/bone healing activities.The most relevant pharmacological properties and their mechanisms of action are summarized in Figure4.Figure 4
Summarized diagram with pharmacological properties ofLepidium sativum and its potential mechanism of actions. Abbreviations and symbols: ↑: increase; ↓: decrease; Bcl-2: B-cell lymphoma 2; GPx: glutathione peroxidase; LDH: lactate dehydrogenase; ROS: reactive oxygen species.


### 5.1. Anticancer

Globally, cancer is the second leading cause of death and modern drugs and techniques used to treat cancer possess several toxicities and side effects [77–79]. Easily available traditional medicines and natural remedies for cancer have less or no side effects relative to modern drugs [80, 81]. Many plant extracts and plant-derived secondary metabolites are presently used to treat cancer and to eliminate the side effects of chemotherapy [82].In the anticancer activity ofL. sativum leave extract (aqueous) against CAL-27, a human tongue squamous carcinoma was evaluated a dose-dependent manner (70, 100, and 150 μg/mL). The best result was shown at 100 and 150 μg/mL of concentrations where the aqueous leaves extract of L. sativum caused significant damage to DNA and increase the apoptosis up to 30% and 60%. The results also showed the increase in reactive oxygen species (ROS) level in the mitochondria of CAL-27 [83]. The hydroalcoholic leave extract of L. sativum showed optimum antiproliferative and apoptotic activity against cervical cancer cell lines (HeLa) cell lines at 100 μg/mL [84].The combination of shoots stems and leave hydroalcoholic extracts before and after flowering was tested for cytotoxic effect against leukemia cell line (K562) at different concentrations ranging between 12.5 and 100μg/mL [85]. The hydroalcoholic extracts before and after flowering exhibit cytotoxic effect against K562 cell lines and the best results are shown at 25 μg/mL of concentration.The methanolic extract ofL. sativum shows cytotoxic effect against lymphocyte cells and colon and endometrium cancer cell lines (DLD-1 and ECC-1) through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay [44]. The necrotic effect, apoptotic activity, and genotoxic activity of plant extract were also investigated by lactate dehydrogenase (LDH), DNA ladder fragmentation, enzyme-linked immunosorbent assay (ELISA), ethidium bromide staining, and comet assay. The extract showed cytotoxic activity in a concentration-dependent manner against colon and endometrium cancer cells; however, the maximum apoptotic and genotoxic activity was seen at 200 μg/mL of a concentration [44].Kassie et al. [86] reported the chemoprotective effect of L. sativum seed extract and their compounds (glucotropaeolin and benzylisothiocyanate) on 2-amino-3-methyl-imidazo [4,5-f] quinoline- (IQ-) induced genotoxic effects and colonic preneoplastic lesions in male Fischer 344 rats. The pretreatment of the experimental model with L. sativum extracts (0.8 mL) and their compounds (GT: 150 mg/kg, BITC: 70 mg/kg) up to three days (consecutive) triggered a significant reduction in IQ-induced DNA damage in colon and liver cells ranging between 75 and 92% [86]. The aqueous seed extract of L. sativum showed cytotoxic effect against breast cancer cell lines (MCF-7) by sulforhodamine B and tryphan blue dye exclusion assay at concentration and time-dependent manner (25%, 50%, and 75%) [87].The cytotoxic activity of seed extracts (chloroform, n-hexane, methanol, and ethyl acetate) ofL. sativum was tested against human neuroblastoma (IMR-32), colon cancer (HT-15 and 29), and lung cancer (A-549) cell lines [88]. The study showed that all the extracts exhibited significant in vitro cytotoxicity against all the cell lines; however, methanolic seed extract shows the highest per cent of growth inhibition activity (90±0.88, 95±0.24, 91±0.20, and 87±0.65) for all the cell lines (IMR-32, HT-15, HT-29, and A-549) [88].The aqueous seed extract ofL. sativum with the lowest and highest concentration of 200 and 400 mg/kg was evaluated for anticancer activity against dextran sulfate sodium/azoxymethane-induced colon cancer in the albino mice model [89]. The result showed that at 400 mg/kg of concentration, the seed extract exhibits higher apoptosis and higher anticancer activity against colon cancer with a decrease in colon tumor/polyp size and incidence and tissue disorder [89]. The seed extract of L. sativum alone and with silver nanoparticles showed significant anticancer activity against HT-29 colon cancer cell lines by inducing apoptosis and mitotic cell arrest. They also increase the p53 expression and prevent cell division of HT-29 colon cancer cells [90].Ait-Yahia et al. [91] studied the cytotoxic effect of aglycones (flavones/flavonoids), C-glycosides, and O-glycosides, isolated from the seed and leave extract of L. sativum against human laryngeal carcinoma cells (HEp2). The findings showed that all the compounds possess cytotoxic activity, whereas the highest cytotoxic effect was observed for the O-glycosylate rich acetate ethyl extract at 57 μg/mL of concentration [91].


### 5.2. Hepatoprotective

The liver is a crucial part of the body that play a fundamental role in different physiological processes and functions including secretion, metabolism, and storage [92]. Numerous studies proved its important role in the detoxification and excretion of endogenous waste metabolites and exogenous toxic compounds from the body [93, 94].The liver is also involved in various biochemical processes of nutrient and energy supply, growth, etc. Additionally, it helps in carbohydrate and fat metabolism, bile secretion, and vitamin storage [95, 96]. However, biological factors, genetic factors, environmental factors, autoimmune diseases, toxic compounds, and chemicals result in damage of the cell, structure, tissues, and functioning of the liver and cause hepatic diseases. Modern drugs can also cause an adverse effect on liver as they possess numerous side effects [97]. Thus, there is a need to identify the alternative treatment of hepatic diseases to discover more effective and less toxic natural agents [98–100].Hepatoprotective activity of the seed and herb extracts (petroleum ether and alcohol) ofL. sativum was evaluated against carbon tetrachloride- (CCl4-) induced toxicity in hepatocytes at different concentrations, and the results showed that both the extracts of seed and herb at a minimum concentration of 50 μg/mL possess a hepatoprotective effect on the hepatocytes against CCl4 cytotoxicity; however, the concentration that prevents the growth of half of the cells was 150 μg/mL and 200 μg/mL, respectively [56]. The results also showed that the alcoholic extract is safer than petroleum ether extract [56].L. sativum seed show in vivo hepatoprotective activity for the prevention of CCl4-induced liver damage in Wistar albino rats at different concentrations ranging from 100 mg/kg to 400 mg/kg body weight [53, 101, 102]. The total alkaloid fraction of seeds of L. sativum was screened for the hepatoprotective activity against CCl4 at 50, 150, and 250 mg/kg (i.p.) of concentrations, and the finding showed that in all concentrations, the extract showed hepatoprotective activity, and the maximum activity was observed at 250 mg/kg [103].Sakran et al. [104] reported in vivo hepatoprotective activity of a new isoflavonoid (5,6-dimethoxy-2′,3′-methylenedioxy-7-C-β-D-gluco-pyranosyl isoflavone) isolated from the seeds of L. sativum against paracetamol-induced hepatotoxicity in Sprague Dawley male rats at 100 mg/kg of dose. Al-Sheddi et al. [105] reported the hepatoprotective effect of chloroform extract of seed of L. sativum at 5, 10, and 25 mg/mL of concentrations against hepatotoxicity induced by hydrogen peroxide in HepG2 cell lines [105].Hepatoprotective activity ofL. sativum seed extract (ethanolic) was evaluated at 150 and 300 mg/kg of doses against D-galactosamine/lipopolysaccharide-induced hepatotoxicity in the Wistar rat model. The result revealed the hepatoprotective activity of the L. sativum seed ethanolic extract and showed that the pretreatment of the extract upregulates Bcl-2 protein expression and downregulated caspase-3 in mice [21].


### 5.3. Antidiabetic

In the last few decades, the global prevalence of diabetes has risen faster not in developed but also in developing countries. Diabetes also causes dysfunction, damage, and failure of a various organ systems which can lead to premature death. Existing synthetic antidiabetic drugs show several limitations and therefore, the search for new antidiabetic agents from natural resources continues [106].The hypoglycemic activity of aqueous seed extract ofL. sativum was evaluated in vivo in streptozotocin-induced diabetic Wistar rats at 20 mg/kg of concentration [19, 107]. The result showed significant hypoglycemic activity in the rat model without showing any effect in basal plasma insulin concentration [19, 107].Mishra et al. [108] also investigated the hypoglycemic activity of seeds of L. sativum on streptozotocin-induced diabetic Wistar rat and showed the reduction in glucose, alkaline phosphate, and creatinine levels at 20 mg/kg of dose [108]. The total alkaloid fraction of L. sativum seed was investigated for antidiabetic activity in alloxan-induced diabetic Wistar rat model at different (50, 150, and 250 mg/kg, i.p.) concentrations [109].Kamani et al. [110] reported that the methanolic seed extract of L. sativum at 200 and 400 mg/kg of doses showed antidiabetic activity against streptozotocin-induced diabetic in albino rats. The fraction suppresses blood glucose, cholesterol, triglyceride, and urea level and showed the best antidiabetic results at 250 mg/kg of concentration [110]. The methanolic seed extract of L. sativum also showed the highest antidiabetic activity against alloxan-induced albino rat at 300 mg/kg of dose [111].


### 5.4. Antioxidant

Plants are the major source of natural antioxidants, which function as free radical scavengers and reducing agents against reactive oxygen species and free radicals [112, 113].The antioxidants present in the plant are found in the form of vitamins, phenols, terpenoids, flavonoids, coumarins, alkaloids, etc.Researchers reported the antioxidant potential ofL. sativum using different important antioxidants like gallic acid, coumarin acid, caffeic acid, quercetin, tocopherol (α, β, γ, δ), and among others [40, 41]. The ethanolic extract of stem, leaves, whole plant, and seeds of L. sativum was tested for antioxidant activity by several methods including 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging assay, reduced glutathione assay, reducing power assay, and ascorbic acid content determination [37]. The result from the study suggests that all the parts (stem, leaves, whole plant, seeds) of L. sativum possess scavenging activity; however, the maximum per cent (12.19%±0.2) was noted for the whole plant, and the minimum per cent (2.69%±0.5) was noted for stem part. In reduced glutathione assay, all the extracts showed enhanced antioxidant activity; however, the highest value was measured in ethanolic leaf extract, i.e., 9 μg/mL. Reducing power or Fe3+-Fe2+ transformation ability assay showed that all the plant parts possess the significant reducing ability [37].Sat, Yildirim, Turan, and Demirbas [36] reported the antioxidant potential of species using DPPH assay in terms of EC50 value (EC50: 330.99 μg/mL (Dadas, Turkey) and 346.65 μg/mL (Dadas, Turkey) for FW and 128.08 and 85.97 μg/mL for DW). However, Al-Saad and Al-Saadi [62] reported the IC50 value of 149.541 μg/mL for the leaves of L. sativum by DPPH assay.The DPPH, ABTS (2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid)), FRAP (ferric [Fe(III)] ion–reducing antioxidant power), andβ-carotene bleaching assays were performed to investigate the antioxidant activity of ethanolic and aqueous seed extract of L. sativum. The results showed 31.15% and 18.07% of DPPH activity and 24.61% and 21.14% ABTS activity for ethanolic and aqueous seed extracts, respectively. The FRAP and β-carotene/linoleic bleaching assay also confirm the antioxidant potential of the ethanolic and aqueous extract of L. sativum [42].Kadam, Palamthodi, and Lele [40] also determined that the ethanolic seed extract of L. sativum possesses significant antioxidant activity using DPPH (IC50: 162.4±2.3μg/mL), ABTS (IC50: 35.29±1.02 μg/mL), superoxide scavenging activity (IC50: 187.12 ± 3.4 μg/mL), and metal chelating property (IC50: 119.32±1.5 μg/mL) assays [40].Chatoui, Harhar, El Kamli, and Tabyaoui [38] evaluated the methanolic and ethanolic seed extracts from Tafraout, Rommani, Ben-Ahmed, and El-Haouz regions in Morocco for the estimation of antioxidant activity. The results showed that the highest DPPH (IC50: 119.3 μg/mL), ABTS (IC50: 187.8 μg/mL), and FRAP (EC50: 777.0 μg/mL) activities in the methanolic seed extract of L. sativum are collected from Tafraout region [38]. Nitric oxide assay, total antioxidant capacity assay, reducing power assay, and hydrogen peroxide scavenging assay of aqueous and ethanolic seed extract of L. sativum showed the presence of significant antioxidant activity [43]. Few more studies from different regions confirm that the seed extract of L. sativum possesses significant amount of antioxidants and antioxidant activity [32, 39, 44, 74, 114, 115].


### 5.5. Antimicrobial

Presently, due to several environmental, biological, physical, chemical, and anthropogenic factors, the incidences of pathogenic microorganisms are increasing constantly, and this became a major concern among several scientific communities [116, 117]. The plant serves as a source of secondary metabolites which possess low or no side effects with other nutritional benefits. The antimicrobial activity of numerous medicinal plants has been studied against a range of microorganisms including bacteria, yeast, fungi, and virus, and many research groups are working continuously to discover novel antimicrobial compounds.Hussain, Khattak, Muhammad, Khan, Khan, Ullah, and Haider [58] studied the antimicrobial activity of aqueous and chloroform plant extracts of L. sativum against a few bacterial strains including Bacillus subtilis, Proteus vulgaris, Pseudomonas aeruginosa, Salmonella typhi, Staphylococcus aureus, Escherichia coli, and two fungal strains, Aspergillus niger and Candida albicans by the agar well diffusion method [58]. The result showed that both the extracts possess antimicrobial activity against all the bacterial and fungal strains; however, the maximum and minimum zone of inhibition (ZI) for chloroform extract was shown by P. vulgaris (13 mm) and S. typhi (1 mm) and for aqueous extract, the maximum and minimum ZI was observed in P. vulgaris (16 mm) and E. coli (2 mm) [58].The root, stem, and leaves were extracted with methanol, ethyl acetate, chloroform, and aqueous solvents and evaluated for antimicrobial activity. All extracts showed antimicrobial activity against bacterial strains (E. coli, S. aureus, Klebsiella pneumoniae, and Salmonella typhimurium) and fungal strains (Aspergillus flavus, Aspergillus fumigatus, A. niger, and Fusarium solani) [118]. Plant extract of L. sativum (ethanolic and aqueous) also showed antimicrobial activity against Proteus spp., S. aureus, and Streptococcus mutants by well diffusion method, whereas by minimum inhibitory concentrations (MIC), all the strains (K. pneumoniae, Proteus spp., S. mutans, P. aeruginosa, and Staphylococcus aureus) found to be sensitive to all concentrations (12.5%, 25%, 50%, 75%, and 100%) of the aqueous and ethanolic extracts of L. sativum [119].The sprouts (dried and freeze dried) extract of the plant (L. sativum) is also examined for their antimicrobial activity against K. pneumoniae, Proteus mirabilis, S. aureus, Salmonella infantis, S. epidermidis, E.coli, and P. aeruginosa through well diffusion method [66]. Among dried and freeze-dried sprout extracts, the best result was observed in freeze-dried sprout extract showing maximum activity for S. aureus (21.5 mm), no activity was seen against K. pneumoniae and E. coli, and the MIC value for freeze-dried extract ranges between 0.5 and 1 mg/mL [66].Ibrahim and Kebede [120] evaluated the antibacterial activities of aqueous and methanolic extracts of leaves of L. sativum against human pathogenic bacteria (S. aureus, S. typhi, Streptococcus agalactiae, Shigella boydii) [120]. Along with leaves, different seed extracts of L. sativum showed potential antimicrobial activity against a series of microbial strains (Table 6).Table 6
Antimicrobial activities of different extracts ofL. sativum.
Extract/concentration
Control drug used
Microorganisms
Agar well diffusion method/agar disc diffusion method ZI (mm); control drug (ZI)
MIC/MBC (mg/mL)
Regions
References
Chloroform extract (100 mg/mL)
Gentamicin
Escherichia coli
Salmonella typhi
Pseudomonas aeruginosa
Staphylococcus aureus
Bacillus cereus
Micrococcus luteus
NZ; 22NZ; 24NZ; 2110; 25NZ; 2811; 27
NT
India
[128]
Ethyl acetate extract (100 mg/mL)
Gentamicin
Escherichia coli
Salmonella typhi
Pseudomonas aeruginosa
Staphylococcus aureus
Bacillus cereus
Micrococcus luteus
14; 22NZ; 24NZ; 2118; 25NZ; 2816; 27
NT
Methanol extract (100 mg/mL)
Gentamicin
Escherichia coli
Salmonella typhi
Pseudomonas aeruginosa
Staphylococcus aureus
Bacillus cereus
Micrococcus luteus
14; 2213; 2414; 2122; 2516; 2816; 27
6.25/2525/ND6.25/251.56/6.256.25/25.012.5/ND
Dichloromethane extract (100 mg/mL)
Gentamicin
Escherichia coli
Salmonella typhi
Pseudomonas aeruginosa
Staphylococcus aureus
Bacillus cereus
Micrococcus luteus
NZ; 22NZ; 24NZ; 21NZ; 25NZ; 2810; 27
NT
Petroleum ether extracts (2.5%)
Gentamicin/ Ketoconzol
Staphylococcus aureus
Escherichia coli
Klebsiella pneumoniae
Proteus vulgaris
Pseudomonas aeruginosa
Candida albicans
25; 3225; 3226; 3521; 3418; 3232; 33
NT
Sudan
[129]
Methanolic extract (2.5%)
Gentamicin/Ketoconzol
Staphylococcus aureus
Escherichia coli
Klebsiella pneumoniae
Proteus vulgaris
Pseudomonas aeruginosa
Candida albicans
15; 3217; 3216; 3518; 3417; 329; 33
Aqueous extract (10%)
Gentamicin/ Ketoconzol
Staphylococcus aureus
Escherichia coli
Klebsiella pneumoniae
Proteus vulgaris
Pseudomonas aeruginosa
Candida albicans
NZ; 3219; 3217; 3519; 3416; 3221; 33
Ethanolic extract (1 mg/mL)
Not mentioned
Staphylococcus aureus
Listeria monocytogenes
Salmonella Enteritidis
Escherichia coli
Serratia marcescens
10∗10∗20∗12∗7
NT
Egypt
[54]
Methanol extract (1 mg/mL)
Not mentioned
Salmonella Enteritidis
Serratia marcescens
159
Methanol extract (100 mg/mL)
Gentamicin/ nystatin
Escherichia coli
Staphylococcus aureus
Bacillus subtilis
Candida albicans
Aspergillus niger
14; 3213; 3513; 2914; 1720; 20
NT
Sudan
[130]
Ethanol extract (50 mg/mL)
Vancomycin
Escherichia coli
Pseudomonas aeruginosa
22.63; 1810; NA
NT
Ethiopia
[131]
Methanol extract (50 mg/mL)
Vancomycin
Escherichia coli
Pseudomonas aeruginosa
22.37; 189; NA
Chloroform extract (50 mg/mL)
Vancomycin
Escherichia coli
Pseudomonas aeruginosa
10.67; 1811.33; NA
Ethyl acetate extract
Erythromycin
Rhodococcus equi
15.5; 23
NT
Morocco
[18]
Methanolic extract
Chlorophénicol
Rhodococcus equi
13.15; 34
Petroleum ether extract
Ciprofloxacine
Rhodococcus equi
10.35; 30
Ethanol extract
Not mentioned
Pseudomonas aeruginosa
Klebsiella pneumonia
Escherichia coli
Staphylococcus aureus
Streptococcus pyogenes
MβL, P. aeruginosaESβL, E. coliMRSAMDR S. pyogenes
NT
12.5/256.25/12.53.13/3.136.25/6.2550/5025/2512.5/12.512.5/25100/50
Egypt
[132]
Acetone extract
Not mentioned
Pseudomonas aeruginosa
Klebsiella pneumonia
Escherichia coli
Staphylococcus aureus
MβL, P. aeruginosaESβL, Klebsiella pneumoniaESβL, E. coliMRSA
25/2512.5/256.25/12.53.13/6.2525/5012.5/12.512.5/2512.5/25
Aqueous extract
Not mentioned
Pseudomonas aeruginosa
Escherichia coli
Staphylococcus aureus
E. coli MRSA
25/256.25/6.256.25/12.512.5/25
n-Butanol extract
Not mentioned
Escherichia coli
Pseudomonas aeruginosa
Staphylococcus aureus (methicillin-sen.)Staphylococcus aureus (methicillin-res.)Enterococcus faecalis
NT
54443.5
Algeria
[64]
Abbreviations: NZ: no zone of inhibition; NA: not applicable; NT: not tested; MBC: minimum bactericidal concentration; MIC: minimum inhibitory concentration.Gacemi et al. [121] reported the antifungal activity of lepidines B and E and compounds present in seeds of L. sativum against of C. albicans. The seed oil of L. sativum possesses antifungal and antibacterial activity against S. aureus, B. subtilis, P.aeruginosa, E. coli, Salmonella enterica, and C. albicans. The essential oil extracted by clevenger type apparatus from seeds of L. sativum showed the best activity at 1 mg/mL of concentration against S. aureus (15.57±0.46 mm ZI), B. cereus (13.12±1.16 mm ZI), E. coli (9.78±065 mm ZI), and K. pneumoniae (8.17±0.32 mm ZI) by disc diffusion assay [121].


### 5.6. Gastroprotective

Gastrointestinal infections are one of the most common problems in tropical countries. They involve various parts of the gastrointestinal tract and organs like the pancreas, liver and gallbladder [122]. They are responsible for causing problems like diarrhoea, abdominal distention, intestinal obstruction, abdominal pain, and gastrointestinal bleeding [123]. Gastrointestinal diseases directly or indirectly have an economic impact and also alter the quality of life Natural active compounds possess preventive and healing activity against gastrointestinal diseases [122, 124].The methanolic extract of seed ofL. sativum at 50, 100, and 200 mg/kg p.o. concentration was investigated for antidiarrheal activity against castor oil-induced diarrhoea in Swiss albino and Wistar rat models [125]. The highest antidiarrheal activity was observed in 200 mg/kg of concentration.Rehman et al. [126] investigated the antidiarrheal and antispasmodic activities of seed extract of L. sativum against castor oil-induced diarrhoea in Sprague Dawley rat model at 100-300 mg/kg of doses [126]. The crude extract of seed was found to possess significant antidiarrheal and antispasmodic activity.Another study by Mehmood et al. [127] investigated the aqueous-methanolic seed extract of L. sativum for indigestion and constipation at 30 and 100 mg/kg of doses in BALB/c mice, guinea pigs, and rabbits. The study showed the laxative and prokinetic effects of L. sativum seeds in the mice model [127].


### 5.7. Fracture/Bone Healing

Fracture healing or bone healing is a complicated physiological process that requires the participation of hematopoietic and immune cells in the bone marrow. Medicinal plants have important properties to reduce inflammation and pain of fractures and also help in fracture fast recovery [133, 134].The impact ofL. sativum seeds on fracture induced bone healing in rabbit (Oryctolagus cuniculus) model was evaluated. The test group had a statistically significant increase in the healing of fractures compared with the control group. The results showed the significant effect of L. sativum seeds in fracture induced bone healing [135]. Yadav et al. [136] reported the effect of ethanol seed extract (400 mg/kg p.o.) of L. sativum on fracture healing in the Wistar rat model.The osteoprotective effect ofL. sativum seeds (doses: 50-100 mg/kg) was studied in an ovary ectomized Wistar rat model [61]. Results revealed the antiosteoporotic actions of L. sativum with improved perpendicular and longitudinal femur compression strength.Extract also enhanced the osteocalcin levels, and serum bone formation biomarkers lactate dehydrogenase (LDH) activity and inhibit the glutathione peroxidase (GPx) activity and deposition of lipid peroxides in bone tissues [61].L. sativum showed a promising protective effect with no side effects against glucocorticoid-induced bone resorption in guinea pigs [137] and accelerates the alveolar bone healing and improves the formation of bone in periodontal diseases [138]. Alharbi et al. [139] investigated the in vivo effect of L. sativum seeds in osteogenic enhancement in bone fractures induced in O. cuniculus and concluded that the seeds can be used in the treatment of bone fractures [139].


## 5.1. Anticancer

Globally, cancer is the second leading cause of death and modern drugs and techniques used to treat cancer possess several toxicities and side effects [77–79]. Easily available traditional medicines and natural remedies for cancer have less or no side effects relative to modern drugs [80, 81]. Many plant extracts and plant-derived secondary metabolites are presently used to treat cancer and to eliminate the side effects of chemotherapy [82].In the anticancer activity ofL. sativum leave extract (aqueous) against CAL-27, a human tongue squamous carcinoma was evaluated a dose-dependent manner (70, 100, and 150 μg/mL). The best result was shown at 100 and 150 μg/mL of concentrations where the aqueous leaves extract of L. sativum caused significant damage to DNA and increase the apoptosis up to 30% and 60%. The results also showed the increase in reactive oxygen species (ROS) level in the mitochondria of CAL-27 [83]. The hydroalcoholic leave extract of L. sativum showed optimum antiproliferative and apoptotic activity against cervical cancer cell lines (HeLa) cell lines at 100 μg/mL [84].The combination of shoots stems and leave hydroalcoholic extracts before and after flowering was tested for cytotoxic effect against leukemia cell line (K562) at different concentrations ranging between 12.5 and 100μg/mL [85]. The hydroalcoholic extracts before and after flowering exhibit cytotoxic effect against K562 cell lines and the best results are shown at 25 μg/mL of concentration.The methanolic extract ofL. sativum shows cytotoxic effect against lymphocyte cells and colon and endometrium cancer cell lines (DLD-1 and ECC-1) through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay [44]. The necrotic effect, apoptotic activity, and genotoxic activity of plant extract were also investigated by lactate dehydrogenase (LDH), DNA ladder fragmentation, enzyme-linked immunosorbent assay (ELISA), ethidium bromide staining, and comet assay. The extract showed cytotoxic activity in a concentration-dependent manner against colon and endometrium cancer cells; however, the maximum apoptotic and genotoxic activity was seen at 200 μg/mL of a concentration [44].Kassie et al. [86] reported the chemoprotective effect of L. sativum seed extract and their compounds (glucotropaeolin and benzylisothiocyanate) on 2-amino-3-methyl-imidazo [4,5-f] quinoline- (IQ-) induced genotoxic effects and colonic preneoplastic lesions in male Fischer 344 rats. The pretreatment of the experimental model with L. sativum extracts (0.8 mL) and their compounds (GT: 150 mg/kg, BITC: 70 mg/kg) up to three days (consecutive) triggered a significant reduction in IQ-induced DNA damage in colon and liver cells ranging between 75 and 92% [86]. The aqueous seed extract of L. sativum showed cytotoxic effect against breast cancer cell lines (MCF-7) by sulforhodamine B and tryphan blue dye exclusion assay at concentration and time-dependent manner (25%, 50%, and 75%) [87].The cytotoxic activity of seed extracts (chloroform, n-hexane, methanol, and ethyl acetate) ofL. sativum was tested against human neuroblastoma (IMR-32), colon cancer (HT-15 and 29), and lung cancer (A-549) cell lines [88]. The study showed that all the extracts exhibited significant in vitro cytotoxicity against all the cell lines; however, methanolic seed extract shows the highest per cent of growth inhibition activity (90±0.88, 95±0.24, 91±0.20, and 87±0.65) for all the cell lines (IMR-32, HT-15, HT-29, and A-549) [88].The aqueous seed extract ofL. sativum with the lowest and highest concentration of 200 and 400 mg/kg was evaluated for anticancer activity against dextran sulfate sodium/azoxymethane-induced colon cancer in the albino mice model [89]. The result showed that at 400 mg/kg of concentration, the seed extract exhibits higher apoptosis and higher anticancer activity against colon cancer with a decrease in colon tumor/polyp size and incidence and tissue disorder [89]. The seed extract of L. sativum alone and with silver nanoparticles showed significant anticancer activity against HT-29 colon cancer cell lines by inducing apoptosis and mitotic cell arrest. They also increase the p53 expression and prevent cell division of HT-29 colon cancer cells [90].Ait-Yahia et al. [91] studied the cytotoxic effect of aglycones (flavones/flavonoids), C-glycosides, and O-glycosides, isolated from the seed and leave extract of L. sativum against human laryngeal carcinoma cells (HEp2). The findings showed that all the compounds possess cytotoxic activity, whereas the highest cytotoxic effect was observed for the O-glycosylate rich acetate ethyl extract at 57 μg/mL of concentration [91].


## 5.2. Hepatoprotective

The liver is a crucial part of the body that play a fundamental role in different physiological processes and functions including secretion, metabolism, and storage [92]. Numerous studies proved its important role in the detoxification and excretion of endogenous waste metabolites and exogenous toxic compounds from the body [93, 94].The liver is also involved in various biochemical processes of nutrient and energy supply, growth, etc. Additionally, it helps in carbohydrate and fat metabolism, bile secretion, and vitamin storage [95, 96]. However, biological factors, genetic factors, environmental factors, autoimmune diseases, toxic compounds, and chemicals result in damage of the cell, structure, tissues, and functioning of the liver and cause hepatic diseases. Modern drugs can also cause an adverse effect on liver as they possess numerous side effects [97]. Thus, there is a need to identify the alternative treatment of hepatic diseases to discover more effective and less toxic natural agents [98–100].Hepatoprotective activity of the seed and herb extracts (petroleum ether and alcohol) ofL. sativum was evaluated against carbon tetrachloride- (CCl4-) induced toxicity in hepatocytes at different concentrations, and the results showed that both the extracts of seed and herb at a minimum concentration of 50 μg/mL possess a hepatoprotective effect on the hepatocytes against CCl4 cytotoxicity; however, the concentration that prevents the growth of half of the cells was 150 μg/mL and 200 μg/mL, respectively [56]. The results also showed that the alcoholic extract is safer than petroleum ether extract [56].L. sativum seed show in vivo hepatoprotective activity for the prevention of CCl4-induced liver damage in Wistar albino rats at different concentrations ranging from 100 mg/kg to 400 mg/kg body weight [53, 101, 102]. The total alkaloid fraction of seeds of L. sativum was screened for the hepatoprotective activity against CCl4 at 50, 150, and 250 mg/kg (i.p.) of concentrations, and the finding showed that in all concentrations, the extract showed hepatoprotective activity, and the maximum activity was observed at 250 mg/kg [103].Sakran et al. [104] reported in vivo hepatoprotective activity of a new isoflavonoid (5,6-dimethoxy-2′,3′-methylenedioxy-7-C-β-D-gluco-pyranosyl isoflavone) isolated from the seeds of L. sativum against paracetamol-induced hepatotoxicity in Sprague Dawley male rats at 100 mg/kg of dose. Al-Sheddi et al. [105] reported the hepatoprotective effect of chloroform extract of seed of L. sativum at 5, 10, and 25 mg/mL of concentrations against hepatotoxicity induced by hydrogen peroxide in HepG2 cell lines [105].Hepatoprotective activity ofL. sativum seed extract (ethanolic) was evaluated at 150 and 300 mg/kg of doses against D-galactosamine/lipopolysaccharide-induced hepatotoxicity in the Wistar rat model. The result revealed the hepatoprotective activity of the L. sativum seed ethanolic extract and showed that the pretreatment of the extract upregulates Bcl-2 protein expression and downregulated caspase-3 in mice [21].


## 5.3. Antidiabetic

In the last few decades, the global prevalence of diabetes has risen faster not in developed but also in developing countries. Diabetes also causes dysfunction, damage, and failure of a various organ systems which can lead to premature death. Existing synthetic antidiabetic drugs show several limitations and therefore, the search for new antidiabetic agents from natural resources continues [106].The hypoglycemic activity of aqueous seed extract ofL. sativum was evaluated in vivo in streptozotocin-induced diabetic Wistar rats at 20 mg/kg of concentration [19, 107]. The result showed significant hypoglycemic activity in the rat model without showing any effect in basal plasma insulin concentration [19, 107].Mishra et al. [108] also investigated the hypoglycemic activity of seeds of L. sativum on streptozotocin-induced diabetic Wistar rat and showed the reduction in glucose, alkaline phosphate, and creatinine levels at 20 mg/kg of dose [108]. The total alkaloid fraction of L. sativum seed was investigated for antidiabetic activity in alloxan-induced diabetic Wistar rat model at different (50, 150, and 250 mg/kg, i.p.) concentrations [109].Kamani et al. [110] reported that the methanolic seed extract of L. sativum at 200 and 400 mg/kg of doses showed antidiabetic activity against streptozotocin-induced diabetic in albino rats. The fraction suppresses blood glucose, cholesterol, triglyceride, and urea level and showed the best antidiabetic results at 250 mg/kg of concentration [110]. The methanolic seed extract of L. sativum also showed the highest antidiabetic activity against alloxan-induced albino rat at 300 mg/kg of dose [111].


## 5.4. Antioxidant

Plants are the major source of natural antioxidants, which function as free radical scavengers and reducing agents against reactive oxygen species and free radicals [112, 113].The antioxidants present in the plant are found in the form of vitamins, phenols, terpenoids, flavonoids, coumarins, alkaloids, etc.Researchers reported the antioxidant potential ofL. sativum using different important antioxidants like gallic acid, coumarin acid, caffeic acid, quercetin, tocopherol (α, β, γ, δ), and among others [40, 41]. The ethanolic extract of stem, leaves, whole plant, and seeds of L. sativum was tested for antioxidant activity by several methods including 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging assay, reduced glutathione assay, reducing power assay, and ascorbic acid content determination [37]. The result from the study suggests that all the parts (stem, leaves, whole plant, seeds) of L. sativum possess scavenging activity; however, the maximum per cent (12.19%±0.2) was noted for the whole plant, and the minimum per cent (2.69%±0.5) was noted for stem part. In reduced glutathione assay, all the extracts showed enhanced antioxidant activity; however, the highest value was measured in ethanolic leaf extract, i.e., 9 μg/mL. Reducing power or Fe3+-Fe2+ transformation ability assay showed that all the plant parts possess the significant reducing ability [37].Sat, Yildirim, Turan, and Demirbas [36] reported the antioxidant potential of species using DPPH assay in terms of EC50 value (EC50: 330.99 μg/mL (Dadas, Turkey) and 346.65 μg/mL (Dadas, Turkey) for FW and 128.08 and 85.97 μg/mL for DW). However, Al-Saad and Al-Saadi [62] reported the IC50 value of 149.541 μg/mL for the leaves of L. sativum by DPPH assay.The DPPH, ABTS (2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid)), FRAP (ferric [Fe(III)] ion–reducing antioxidant power), andβ-carotene bleaching assays were performed to investigate the antioxidant activity of ethanolic and aqueous seed extract of L. sativum. The results showed 31.15% and 18.07% of DPPH activity and 24.61% and 21.14% ABTS activity for ethanolic and aqueous seed extracts, respectively. The FRAP and β-carotene/linoleic bleaching assay also confirm the antioxidant potential of the ethanolic and aqueous extract of L. sativum [42].Kadam, Palamthodi, and Lele [40] also determined that the ethanolic seed extract of L. sativum possesses significant antioxidant activity using DPPH (IC50: 162.4±2.3μg/mL), ABTS (IC50: 35.29±1.02 μg/mL), superoxide scavenging activity (IC50: 187.12 ± 3.4 μg/mL), and metal chelating property (IC50: 119.32±1.5 μg/mL) assays [40].Chatoui, Harhar, El Kamli, and Tabyaoui [38] evaluated the methanolic and ethanolic seed extracts from Tafraout, Rommani, Ben-Ahmed, and El-Haouz regions in Morocco for the estimation of antioxidant activity. The results showed that the highest DPPH (IC50: 119.3 μg/mL), ABTS (IC50: 187.8 μg/mL), and FRAP (EC50: 777.0 μg/mL) activities in the methanolic seed extract of L. sativum are collected from Tafraout region [38]. Nitric oxide assay, total antioxidant capacity assay, reducing power assay, and hydrogen peroxide scavenging assay of aqueous and ethanolic seed extract of L. sativum showed the presence of significant antioxidant activity [43]. Few more studies from different regions confirm that the seed extract of L. sativum possesses significant amount of antioxidants and antioxidant activity [32, 39, 44, 74, 114, 115].


## 5.5. Antimicrobial

Presently, due to several environmental, biological, physical, chemical, and anthropogenic factors, the incidences of pathogenic microorganisms are increasing constantly, and this became a major concern among several scientific communities [116, 117]. The plant serves as a source of secondary metabolites which possess low or no side effects with other nutritional benefits. The antimicrobial activity of numerous medicinal plants has been studied against a range of microorganisms including bacteria, yeast, fungi, and virus, and many research groups are working continuously to discover novel antimicrobial compounds.Hussain, Khattak, Muhammad, Khan, Khan, Ullah, and Haider [58] studied the antimicrobial activity of aqueous and chloroform plant extracts of L. sativum against a few bacterial strains including Bacillus subtilis, Proteus vulgaris, Pseudomonas aeruginosa, Salmonella typhi, Staphylococcus aureus, Escherichia coli, and two fungal strains, Aspergillus niger and Candida albicans by the agar well diffusion method [58]. The result showed that both the extracts possess antimicrobial activity against all the bacterial and fungal strains; however, the maximum and minimum zone of inhibition (ZI) for chloroform extract was shown by P. vulgaris (13 mm) and S. typhi (1 mm) and for aqueous extract, the maximum and minimum ZI was observed in P. vulgaris (16 mm) and E. coli (2 mm) [58].The root, stem, and leaves were extracted with methanol, ethyl acetate, chloroform, and aqueous solvents and evaluated for antimicrobial activity. All extracts showed antimicrobial activity against bacterial strains (E. coli, S. aureus, Klebsiella pneumoniae, and Salmonella typhimurium) and fungal strains (Aspergillus flavus, Aspergillus fumigatus, A. niger, and Fusarium solani) [118]. Plant extract of L. sativum (ethanolic and aqueous) also showed antimicrobial activity against Proteus spp., S. aureus, and Streptococcus mutants by well diffusion method, whereas by minimum inhibitory concentrations (MIC), all the strains (K. pneumoniae, Proteus spp., S. mutans, P. aeruginosa, and Staphylococcus aureus) found to be sensitive to all concentrations (12.5%, 25%, 50%, 75%, and 100%) of the aqueous and ethanolic extracts of L. sativum [119].The sprouts (dried and freeze dried) extract of the plant (L. sativum) is also examined for their antimicrobial activity against K. pneumoniae, Proteus mirabilis, S. aureus, Salmonella infantis, S. epidermidis, E.coli, and P. aeruginosa through well diffusion method [66]. Among dried and freeze-dried sprout extracts, the best result was observed in freeze-dried sprout extract showing maximum activity for S. aureus (21.5 mm), no activity was seen against K. pneumoniae and E. coli, and the MIC value for freeze-dried extract ranges between 0.5 and 1 mg/mL [66].Ibrahim and Kebede [120] evaluated the antibacterial activities of aqueous and methanolic extracts of leaves of L. sativum against human pathogenic bacteria (S. aureus, S. typhi, Streptococcus agalactiae, Shigella boydii) [120]. Along with leaves, different seed extracts of L. sativum showed potential antimicrobial activity against a series of microbial strains (Table 6).Table 6
Antimicrobial activities of different extracts ofL. sativum.
Extract/concentration
Control drug used
Microorganisms
Agar well diffusion method/agar disc diffusion method ZI (mm); control drug (ZI)
MIC/MBC (mg/mL)
Regions
References
Chloroform extract (100 mg/mL)
Gentamicin
Escherichia coli
Salmonella typhi
Pseudomonas aeruginosa
Staphylococcus aureus
Bacillus cereus
Micrococcus luteus
NZ; 22NZ; 24NZ; 2110; 25NZ; 2811; 27
NT
India
[128]
Ethyl acetate extract (100 mg/mL)
Gentamicin
Escherichia coli
Salmonella typhi
Pseudomonas aeruginosa
Staphylococcus aureus
Bacillus cereus
Micrococcus luteus
14; 22NZ; 24NZ; 2118; 25NZ; 2816; 27
NT
Methanol extract (100 mg/mL)
Gentamicin
Escherichia coli
Salmonella typhi
Pseudomonas aeruginosa
Staphylococcus aureus
Bacillus cereus
Micrococcus luteus
14; 2213; 2414; 2122; 2516; 2816; 27
6.25/2525/ND6.25/251.56/6.256.25/25.012.5/ND
Dichloromethane extract (100 mg/mL)
Gentamicin
Escherichia coli
Salmonella typhi
Pseudomonas aeruginosa
Staphylococcus aureus
Bacillus cereus
Micrococcus luteus
NZ; 22NZ; 24NZ; 21NZ; 25NZ; 2810; 27
NT
Petroleum ether extracts (2.5%)
Gentamicin/ Ketoconzol
Staphylococcus aureus
Escherichia coli
Klebsiella pneumoniae
Proteus vulgaris
Pseudomonas aeruginosa
Candida albicans
25; 3225; 3226; 3521; 3418; 3232; 33
NT
Sudan
[129]
Methanolic extract (2.5%)
Gentamicin/Ketoconzol
Staphylococcus aureus
Escherichia coli
Klebsiella pneumoniae
Proteus vulgaris
Pseudomonas aeruginosa
Candida albicans
15; 3217; 3216; 3518; 3417; 329; 33
Aqueous extract (10%)
Gentamicin/ Ketoconzol
Staphylococcus aureus
Escherichia coli
Klebsiella pneumoniae
Proteus vulgaris
Pseudomonas aeruginosa
Candida albicans
NZ; 3219; 3217; 3519; 3416; 3221; 33
Ethanolic extract (1 mg/mL)
Not mentioned
Staphylococcus aureus
Listeria monocytogenes
Salmonella Enteritidis
Escherichia coli
Serratia marcescens
10∗10∗20∗12∗7
NT
Egypt
[54]
Methanol extract (1 mg/mL)
Not mentioned
Salmonella Enteritidis
Serratia marcescens
159
Methanol extract (100 mg/mL)
Gentamicin/ nystatin
Escherichia coli
Staphylococcus aureus
Bacillus subtilis
Candida albicans
Aspergillus niger
14; 3213; 3513; 2914; 1720; 20
NT
Sudan
[130]
Ethanol extract (50 mg/mL)
Vancomycin
Escherichia coli
Pseudomonas aeruginosa
22.63; 1810; NA
NT
Ethiopia
[131]
Methanol extract (50 mg/mL)
Vancomycin
Escherichia coli
Pseudomonas aeruginosa
22.37; 189; NA
Chloroform extract (50 mg/mL)
Vancomycin
Escherichia coli
Pseudomonas aeruginosa
10.67; 1811.33; NA
Ethyl acetate extract
Erythromycin
Rhodococcus equi
15.5; 23
NT
Morocco
[18]
Methanolic extract
Chlorophénicol
Rhodococcus equi
13.15; 34
Petroleum ether extract
Ciprofloxacine
Rhodococcus equi
10.35; 30
Ethanol extract
Not mentioned
Pseudomonas aeruginosa
Klebsiella pneumonia
Escherichia coli
Staphylococcus aureus
Streptococcus pyogenes
MβL, P. aeruginosaESβL, E. coliMRSAMDR S. pyogenes
NT
12.5/256.25/12.53.13/3.136.25/6.2550/5025/2512.5/12.512.5/25100/50
Egypt
[132]
Acetone extract
Not mentioned
Pseudomonas aeruginosa
Klebsiella pneumonia
Escherichia coli
Staphylococcus aureus
MβL, P. aeruginosaESβL, Klebsiella pneumoniaESβL, E. coliMRSA
25/2512.5/256.25/12.53.13/6.2525/5012.5/12.512.5/2512.5/25
Aqueous extract
Not mentioned
Pseudomonas aeruginosa
Escherichia coli
Staphylococcus aureus
E. coli MRSA
25/256.25/6.256.25/12.512.5/25
n-Butanol extract
Not mentioned
Escherichia coli
Pseudomonas aeruginosa
Staphylococcus aureus (methicillin-sen.)Staphylococcus aureus (methicillin-res.)Enterococcus faecalis
NT
54443.5
Algeria
[64]
Abbreviations: NZ: no zone of inhibition; NA: not applicable; NT: not tested; MBC: minimum bactericidal concentration; MIC: minimum inhibitory concentration.Gacemi et al. [121] reported the antifungal activity of lepidines B and E and compounds present in seeds of L. sativum against of C. albicans. The seed oil of L. sativum possesses antifungal and antibacterial activity against S. aureus, B. subtilis, P.aeruginosa, E. coli, Salmonella enterica, and C. albicans. The essential oil extracted by clevenger type apparatus from seeds of L. sativum showed the best activity at 1 mg/mL of concentration against S. aureus (15.57±0.46 mm ZI), B. cereus (13.12±1.16 mm ZI), E. coli (9.78±065 mm ZI), and K. pneumoniae (8.17±0.32 mm ZI) by disc diffusion assay [121].


## 5.6. Gastroprotective

Gastrointestinal infections are one of the most common problems in tropical countries. They involve various parts of the gastrointestinal tract and organs like the pancreas, liver and gallbladder [122]. They are responsible for causing problems like diarrhoea, abdominal distention, intestinal obstruction, abdominal pain, and gastrointestinal bleeding [123]. Gastrointestinal diseases directly or indirectly have an economic impact and also alter the quality of life Natural active compounds possess preventive and healing activity against gastrointestinal diseases [122, 124].The methanolic extract of seed ofL. sativum at 50, 100, and 200 mg/kg p.o. concentration was investigated for antidiarrheal activity against castor oil-induced diarrhoea in Swiss albino and Wistar rat models [125]. The highest antidiarrheal activity was observed in 200 mg/kg of concentration.Rehman et al. [126] investigated the antidiarrheal and antispasmodic activities of seed extract of L. sativum against castor oil-induced diarrhoea in Sprague Dawley rat model at 100-300 mg/kg of doses [126]. The crude extract of seed was found to possess significant antidiarrheal and antispasmodic activity.Another study by Mehmood et al. [127] investigated the aqueous-methanolic seed extract of L. sativum for indigestion and constipation at 30 and 100 mg/kg of doses in BALB/c mice, guinea pigs, and rabbits. The study showed the laxative and prokinetic effects of L. sativum seeds in the mice model [127].


## 5.7. Fracture/Bone Healing

Fracture healing or bone healing is a complicated physiological process that requires the participation of hematopoietic and immune cells in the bone marrow. Medicinal plants have important properties to reduce inflammation and pain of fractures and also help in fracture fast recovery [133, 134].The impact ofL. sativum seeds on fracture induced bone healing in rabbit (Oryctolagus cuniculus) model was evaluated. The test group had a statistically significant increase in the healing of fractures compared with the control group. The results showed the significant effect of L. sativum seeds in fracture induced bone healing [135]. Yadav et al. [136] reported the effect of ethanol seed extract (400 mg/kg p.o.) of L. sativum on fracture healing in the Wistar rat model.The osteoprotective effect ofL. sativum seeds (doses: 50-100 mg/kg) was studied in an ovary ectomized Wistar rat model [61]. Results revealed the antiosteoporotic actions of L. sativum with improved perpendicular and longitudinal femur compression strength.Extract also enhanced the osteocalcin levels, and serum bone formation biomarkers lactate dehydrogenase (LDH) activity and inhibit the glutathione peroxidase (GPx) activity and deposition of lipid peroxides in bone tissues [61].L. sativum showed a promising protective effect with no side effects against glucocorticoid-induced bone resorption in guinea pigs [137] and accelerates the alveolar bone healing and improves the formation of bone in periodontal diseases [138]. Alharbi et al. [139] investigated the in vivo effect of L. sativum seeds in osteogenic enhancement in bone fractures induced in O. cuniculus and concluded that the seeds can be used in the treatment of bone fractures [139].


## 6. The Challenge of Standardizing Extract, Toxicity, and Bioavailability of the Extract

Medicinal plants have shown immense pharmacological activities like fungicidal, bactericidal, virucidal, analgesic, anticancer, anti-inflammatory, neuroprotective, sedative, and antioxidant, due to the presence of significant phytochemicals or active compounds including flavonoids, phenols, terpenoids alkaloids, tannins, saponins, and glycosides [140–143].Presently, excessive use of synthetic drugs and antibiotics has developed serious side effects, toxicity, and resistance against pathogenic microorganisms, which has limited their use in many countries; therefore, researchers are now paying more attention to traditional herbal medicines and their active compounds to fight against diseases and disorders [144–146].One of the main clinical challenge is the reduced bioavailability and absorption of bioactive compounds from plants. As a result, their inclusion in nanoformulations with increased absorption, bioavailability, and transport to the target was the optimal therapeutic solution.Bloukh et al. (2021) evaluate the antimicrobial potential ofLepidium sativum silver nanoparticles against a series of microbes by using agar well and disk diffusion assays. Pure extract and Lepidium sativum silver nanoparticle formulations displayed a significant antimicrobial activity (very good to intermediate) against 10 microbial strains (S. pneumoniae, S. aureus, S. pyogenes, E. faecalis, B. subtilis, P. mirabilis, P. aeruginosa, E. coli, K. pneumoniae, C. albicans) at the concentrations of 1.08 μg/mL, 0.54 μg/mL, and 0.27 μg/mL [147].Yasin et al. [148] evaluated the cytotoxicity of nanocapsulated lectin isolated from L. sativum against hepatocellular carcinoma cells (HepG2). The methanolic seed extract of L. sativum showed anticancer activity against in vivo Ehrlich ascite carcinoma (EAC) cell lines in Swiss albino mice at 500 mg/kg body weight of concentration [149].L. sativum seed acetone extract and its combination with biogenic silver nanoparticles were found to be nontoxic to splenic cells [90].


## 7. Concluding Remarks

The current review discussed the traditional uses, nutritional values, chemical composition, and biological activity ofL. sativum. Under this study, we summarized the presence of important minerals (potassium, calcium, phosphorus, iron, etc), amino acids (glutamic acid, leucine, etc.), fatty acid and essential oils (oleic acid, linoleic acid, linolenic acid, alpha-pinene, gamma-terpinene, alpha-terpineol, sabinene, alpha-phellandrene, etc.), and other secondary metabolites like campesterol, glucosinolates, napthalenol, furfural, coumarin, flavonoid, and phenolic acid in different extracts of L. sativum. The study also shows that it is an important edible herb that possesses wide range of therapeutic properties and high nutraceutical potential and can be used against malnutrition. However, most of the studies are restricted to in vitro studies and very few in vivo. Therefore, further research is needed to develop new phytopharmaceuticals based on L. sativum, and well-designed clinical studies are necessary to validate the biological activities reported in preclinical models mentioned in this review. Other than these scientific perspectives, people participation is needed regarding the planting, conservation, and sustainable use of L. sativum as a source of nutritionally rich food. Based on the scientific evidence, it can be concluded that L. sativum is a rich source of nutritional components along with bioactive compounds and could be used as a functional food.



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