# Evaluating Freshness Loss of Green Tea withQ10 Method and Weibull Hazard Analysis under Accelerated Shelf Life Testing

Authors: Mengxuan Xiao; Sitong Liu; Huozhu Jin; Mingji Xiao; Huiqiang Wang; Haiwei Zhang; Qianying Dai
Journal: Journal of Chemistry
 (2022)

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## Abstract

The important quality of green tea is freshness, but high temperature, light, oxygen, and humidity during storage may reduce the freshness of green tea. Thus, this study investigated the freshness loss of green tea (FLGT) under an accelerated shelf-life testing (ASLT) by sensory evaluation and acceptability test. The FLGTs of the samples stored at 20°C, 30°C, and 40°C, were determined as 67 days, 55 days, and 45 days, respectively by theQ10 method. In addition, they were further determined as 67 days, 57 days, and 42 days, respectively, by the Weibull Hazard Analysis (WHA). The sensory evaluation and acceptability test confirmed the correctness of the above results by 2-alternative choice analysis and chemical analysis. The Q10 method was applied to predict the FLGTs stored at 4°C and −20°C which were 93 days and 150 days, which was further verified by 2-alternative choice analysis and chemical analysis. The results show that it is possible to predict the suitable drinking period of green tea during storage by the Q10 method.


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## Body

## 1. Introduction

Green tea (Camellia sinensis (L.) O. Ktze) is one of the most popular functional beverages in the world because of its antioxidant and anticancer activities and prevention of metabolic disease and neurodegenerative disease [1–4]. Basically, fresh green tea leaves normally were thermally treated to inactivate the endogenous enzymes and dehydration after harvest to yield dried green tea, and such treatments can preserve bioactive compounds and freshness attributes of green tea [5]. While green tea would lose its freshness by high temperature, light, oxygen, and humidity during storage, when green tea loses its freshness, it exhibits dark yellow color with an obviously stale aroma [6], which may decrease pharmacological activity [7]. Therefore, it is significantly important to establish a reliable method on evaluating the freshness loss of green tea (FLGT) during storage.Accelerated shelf-life testing (ASLT) was conducted and temperature sensitivity (Q10) was measured to investigate the freshness of food [8]. Accelerated shelf-life testing (ASLT) is a process of testing a product under accelerated conditions to predict the shelf life of a product under typical storage conditions [9]. Basically, elevated temperature condition is normally applied to the ASLT to expedite the deterioration process, whereas Q10 method is used to estimate the shelf life of a product through the chemical reaction rate alteration in response to every 10-degree rise in temperature [10]. Sensory evaluation plays an important role in the ASLT, and quality-based method and survival analysis are two common methods used in the sensory evaluation [9]. In the quality-based method, the quality difference will be established between the fresh product and products treated through ASLT [9]. For example, a seven-point scale judgement has been established by Hough and Freitas between the fresh and stored commercial ricotta cheese with 0 point as no difference and 6 points as the biggest difference [11].Survival analysis in ASLT refers to a sensory judgement on the acceptance of a product by customers after accelerated treatment, and Weibull Hazard Analysis (WHA) is a normal analytical approach for the sensory judgement [9]. It has been accepted that the quality turning point for a product after accelerated treatment will be determined when its sensory acceptance fails from 50% of the panelists [12]. For example, an investigation on the storage period of fresh-cut apples quality using WHA has indicated that the fresh-cut apples stored at 4°C could have 7.5-day shelf life [13]. It has been reported that Njangsa seed oil after a 21-week shelf-life period will not be accepted by more than 50% of panel judges in terms of its taste under WHA [14].Few studies have been conducted to determine the FLGT with storage process to the best of our knowledge. Therefore, this paper applies ASLT to investigate the FLGT by sensory evaluation, theQ10 method, and WHA, for establishing a reliable approach to predict the FLGT.


## 2. Materials and Methods

### 2.1. Green Tea Samples and Chemicals

Huangshan Maofeng green tea (Camellia sinensis (L.) O. Ktze) is used as the sample in the present study. The green tea samples are provided from West Huangshan Tea Manufacturer (Huangshan, Anhui, China). According to the manufacturer, the fresh green tea leaves after harvest are immediately fixed using the thermal treatment to prevent green tea leaves from fermentation under the standard operating procedure of the green tea production. Afterwards, the green tea leaves are dehydrated to a moisture content below 5.73%, and then the dehydrated green tea leaves (9.0 kg) are immediately packed in 50 g/aluminum foil zip-lock bag with vacuum.These green tea samples are randomly divided into 5 groups and stored at −20°C, 4°C, 20°C, 30°C, and 40°C. The stored green tea samples at −20°C can be considered as fresh tea, and the stored green tea samples at 20°C, 30°C, and 40°C, are prepared for theQ10 method and WHA in ASLT. The freshness duration of the stored green tea samples at 4°C will be evaluated and predicted.Folin-Ciocalteu reagent was purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China). (+)-Catechin (C), (-)-epicatechin (EC), (-)-epigallocatechin (EGC), (-)-gallocatechin (GC), (-)-epicatechin gallate (ECG), (-)-gallocatechin gallate (GCG), EGCG, and GA were purchased from Sigma-Aldrich (St. Louis, MO, USA). HPLC grade methanol and acetonitrile were a product of Tedia Co., Ltd. (Fairfield, OH, USA).


### 2.2. Establishing Nine-Point Scale by Sensory Evaluation

A sensory evaluation is carried out in a panel room with constant temperature (25°C) and humidity (75%) in the State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University. Each tea sample (3.0 g) stored at 20°C, 30°C, and 40°C is extracted with 150 mL of boiling water for 4 min. The tea appearance and the color, aroma, and taste of tea infusion are compared with the fresh control tea leaves (stored at −20°C). A total of 6 profession panelists (a man and five women) are asked to describe their difference, from which the nine-point scale will be established according to a published method [11] with minor modifications. The overall tea sensory quality, including the appearances of tea leaves, the tea infusion color, aroma, and taste with weight coefficients of 30%, 20%, 30%, and 20%, respectively, has been evaluated by weighted sum method.


### 2.3. Evaluating the FLGT by theQ10 Method

Q10 is defined as the temperature sensitivity of a reaction, which describes the increase of reaction (or growth) rate or the decrease of shelf life for a 10°C increase in temperature, and it is usually assumed to be constant over a narrow range of temperature. It has been used to predict quality or nutrient losses for many foods [15]. Here, it is used to determine the FLGT of the green tea samples in ASLT. Q10 of the green tea samples can be calculated using the following equation [16]:(1)Q10ΔT/10=θT1θT2,ΔT=T2−T1,where T1 and T2 represent storage temperatures at 30°C and 40°C, respectively, and θT1 and θT2 are found to be the times when the tea samples stored at 30°C and 40°C lose their freshness according to the weighted score in the sensory evaluation, respectively.


### 2.4. Evaluating the FLGT by WHA

The WHA of these tea samples has been carried out according to a published method with minor modifications using the same panelists in tea sensory evaluation [17]. A total of 10 panelists, 4 males and 6 females with an age range of 20 to 40 years, have the basic knowledge on the sensory evaluation and complete a 2-hour tea sensory evaluation training program. There are two panelists in the beginning of the sensory evaluation. In the process of sensory evaluation, if all the panelists accept the sample to be fresh, just one more panelist is added in the next sensory evaluation. If there are unacceptable attitudes, the number of unacceptable panelists was also extra added except the former one panelist in the following sensory evaluation [18]. The panelists considered that all sensory attributes of the tea sample can be accepted by putting “✓” after the name of the tea sample; otherwise, they were marked with “✕,” and the sensory evaluation would be conducted every five days until more than 50% of the panelists thought the tea sample was unaccepted. Let hx denote a hazard value at time x obtained by(2)hx=1k100,where k is the reverse rank of failed products (rank k and hx are given by columns 1 and 3 in Table 1). For each failure time, xi, the cumulative hazard value is calculated by summing the hazard value and all preceding hazard values. The cumulative hazard can be obtained from the following equation:(3)hT=∑I=iIhti.Table 1
Weibull hazard ranking table for tea samples under the ASLT (20°C, 30°C, and 40°C).
Storage temperature (°C)Reverse rankkAge at termination (days)Hazard 100\kCumulative hazard (%)2012508.338.3311509.0917.42106010.0027.4296511.1138.5486512.5051.0476514.2965.3266716.6781.9956720.00101.9946725.00126.9936733.33160.3226750.00210.32167100.00310.323018405.565.5617405.8811.4416456.2517.6915456.6724.3514507.1431.5013507.6939.1912508.3347.5211509.0956.61105010.0066.6195011.1177.7385512.5090.2375514.29104.5165516.67121.1855520.00141.1845525.00166.1835533.33199.5125550.00249.51155100.00349.514017255.885.8816256.2512.1315306.6718.8014307.1425.9413357.6933.6312358.3341.9711409.0951.06104010.0061.0694011.1172.1784512.5084.6774514.2998.9664516.67115.6254520.00135.6244525.00160.6234533.33193.9624550.00243.96145100.00343.96It should be noted that the cumulative hazard values can be larger than 100%. The cumulative distribution functionF(t) of the Weibull model can be expressed as(4)Ft=1−exp−−tαβ.The relationship betweenH(t) and F(t) can be expressed in the following form:(5)Ht=−ln1−Ft=−tαβ.So, we can perform data analysis based on this formula [19].This probability corresponds to a Weibull percentile of 69.3%. The shape factor (β) is determined as 1/slope. The range of β is 2 < β < 4, which leads to better estimations of shelf life. When the test is extended beyond shelf life, most of samples are judged as unacceptable shifting β outside the optimum range [18].


### 2.5. Verifying the FLGT by 2-Alternative Choice

The comparison between fresh green tea and stale green tea at 20°C, 30°C, and 40°C is carried out using the 2-alternative choice analysis based on a published method [20]. A total of 20 undergraduates majored in Tea Science in Anhui Agricultural University (Hefei, Anhui, China) participated in this experiment. These students were 9 males and 11 females and their ages were between 20 and 30 years. These panelists have basic sensory evaluation knowledge and have been trained specifically on distinguishing the fresh and stale odor from green tea. It has been reported that a minimum number of correct judgements to establish significance at various probability levels for paired difference was 15 out of 20 total participants under a significance level of 0.05 [21]. During the analysis, two tea samples are randomly numbered and given to panelists. The panelists are asked to judge whether the two tea samples are both fresh. The FLGT is determined at regular storage temperature by the Q10 method. The green tea samples stored at −20°C and 4°C for 93 days and 150 days, respectively, have been compared in terms of the appearances of the leaves and the infusion colors, aromas, and tastes under a professional panel. These panelists will be asked to give score according to a nine-point scale.


### 2.6. Verifying the FLGT by Chemical Analysis

Contents of tea polyphenols (TPs) were determined by the Chinese National Standard GB/T 8313-2008. Catechins, including C, EC, EGC, GC, ECG, GCG, and EGCG, in the tea leaves were analyzed on an Agilent 1260 HPLC system. A Venusil C18 column (4.6 × 250 mm, 5 μm) was used to separate these compounds (Agilent Technologies, Santa Clara, CA, USA) under a 1.0 mL/min flow rate. Mobile phase consisted of (A) acetonitrile and (B) 1% acetic acid (v/v) in acetonitrile. A gradient program was as follows: 0 to 7 min, 10% to 30% A; 7 to 10 min, 30% A isocratic; 10 to 12 min, 30% to 60% A; 12 to 15 min, 60% to 10% A; and 15 to 20 min, 10% A isocratic. The column was maintained at 40°C during the gradient program. The injection volume was set at 5 μL and the wavelength of the ultraviolet detector was 278 nm. External standard, catechin, was used for the quantitation of catechins in the tea infusion, respectively. All data were repeated three times and recorded as mean ± standard deviation (SD).


### 2.7. Statistical Analysis

Data have been expressed as the mean ± standard deviation (SD) of triplicate tests. The plot between Weibull percentiles and storage time has been drafted using Origin 9.0 software (Origin Lab Corp., Northampton, MA, USA). Heat map was performed using TBtools software (https://github.com/CJ-Chen/TBtools). The 2-alternative choice data have been programmed into minimum numbers of correct judgements to establish significance at various probability levels according to a published method [21].


## 2.1. Green Tea Samples and Chemicals

Huangshan Maofeng green tea (Camellia sinensis (L.) O. Ktze) is used as the sample in the present study. The green tea samples are provided from West Huangshan Tea Manufacturer (Huangshan, Anhui, China). According to the manufacturer, the fresh green tea leaves after harvest are immediately fixed using the thermal treatment to prevent green tea leaves from fermentation under the standard operating procedure of the green tea production. Afterwards, the green tea leaves are dehydrated to a moisture content below 5.73%, and then the dehydrated green tea leaves (9.0 kg) are immediately packed in 50 g/aluminum foil zip-lock bag with vacuum.These green tea samples are randomly divided into 5 groups and stored at −20°C, 4°C, 20°C, 30°C, and 40°C. The stored green tea samples at −20°C can be considered as fresh tea, and the stored green tea samples at 20°C, 30°C, and 40°C, are prepared for theQ10 method and WHA in ASLT. The freshness duration of the stored green tea samples at 4°C will be evaluated and predicted.Folin-Ciocalteu reagent was purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China). (+)-Catechin (C), (-)-epicatechin (EC), (-)-epigallocatechin (EGC), (-)-gallocatechin (GC), (-)-epicatechin gallate (ECG), (-)-gallocatechin gallate (GCG), EGCG, and GA were purchased from Sigma-Aldrich (St. Louis, MO, USA). HPLC grade methanol and acetonitrile were a product of Tedia Co., Ltd. (Fairfield, OH, USA).


## 2.2. Establishing Nine-Point Scale by Sensory Evaluation

A sensory evaluation is carried out in a panel room with constant temperature (25°C) and humidity (75%) in the State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University. Each tea sample (3.0 g) stored at 20°C, 30°C, and 40°C is extracted with 150 mL of boiling water for 4 min. The tea appearance and the color, aroma, and taste of tea infusion are compared with the fresh control tea leaves (stored at −20°C). A total of 6 profession panelists (a man and five women) are asked to describe their difference, from which the nine-point scale will be established according to a published method [11] with minor modifications. The overall tea sensory quality, including the appearances of tea leaves, the tea infusion color, aroma, and taste with weight coefficients of 30%, 20%, 30%, and 20%, respectively, has been evaluated by weighted sum method.


## 2.3. Evaluating the FLGT by theQ10 Method

Q10 is defined as the temperature sensitivity of a reaction, which describes the increase of reaction (or growth) rate or the decrease of shelf life for a 10°C increase in temperature, and it is usually assumed to be constant over a narrow range of temperature. It has been used to predict quality or nutrient losses for many foods [15]. Here, it is used to determine the FLGT of the green tea samples in ASLT. Q10 of the green tea samples can be calculated using the following equation [16]:(1)Q10ΔT/10=θT1θT2,ΔT=T2−T1,where T1 and T2 represent storage temperatures at 30°C and 40°C, respectively, and θT1 and θT2 are found to be the times when the tea samples stored at 30°C and 40°C lose their freshness according to the weighted score in the sensory evaluation, respectively.


## 2.4. Evaluating the FLGT by WHA

The WHA of these tea samples has been carried out according to a published method with minor modifications using the same panelists in tea sensory evaluation [17]. A total of 10 panelists, 4 males and 6 females with an age range of 20 to 40 years, have the basic knowledge on the sensory evaluation and complete a 2-hour tea sensory evaluation training program. There are two panelists in the beginning of the sensory evaluation. In the process of sensory evaluation, if all the panelists accept the sample to be fresh, just one more panelist is added in the next sensory evaluation. If there are unacceptable attitudes, the number of unacceptable panelists was also extra added except the former one panelist in the following sensory evaluation [18]. The panelists considered that all sensory attributes of the tea sample can be accepted by putting “✓” after the name of the tea sample; otherwise, they were marked with “✕,” and the sensory evaluation would be conducted every five days until more than 50% of the panelists thought the tea sample was unaccepted. Let hx denote a hazard value at time x obtained by(2)hx=1k100,where k is the reverse rank of failed products (rank k and hx are given by columns 1 and 3 in Table 1). For each failure time, xi, the cumulative hazard value is calculated by summing the hazard value and all preceding hazard values. The cumulative hazard can be obtained from the following equation:(3)hT=∑I=iIhti.Table 1
Weibull hazard ranking table for tea samples under the ASLT (20°C, 30°C, and 40°C).
Storage temperature (°C)Reverse rankkAge at termination (days)Hazard 100\kCumulative hazard (%)2012508.338.3311509.0917.42106010.0027.4296511.1138.5486512.5051.0476514.2965.3266716.6781.9956720.00101.9946725.00126.9936733.33160.3226750.00210.32167100.00310.323018405.565.5617405.8811.4416456.2517.6915456.6724.3514507.1431.5013507.6939.1912508.3347.5211509.0956.61105010.0066.6195011.1177.7385512.5090.2375514.29104.5165516.67121.1855520.00141.1845525.00166.1835533.33199.5125550.00249.51155100.00349.514017255.885.8816256.2512.1315306.6718.8014307.1425.9413357.6933.6312358.3341.9711409.0951.06104010.0061.0694011.1172.1784512.5084.6774514.2998.9664516.67115.6254520.00135.6244525.00160.6234533.33193.9624550.00243.96145100.00343.96It should be noted that the cumulative hazard values can be larger than 100%. The cumulative distribution functionF(t) of the Weibull model can be expressed as(4)Ft=1−exp−−tαβ.The relationship betweenH(t) and F(t) can be expressed in the following form:(5)Ht=−ln1−Ft=−tαβ.So, we can perform data analysis based on this formula [19].This probability corresponds to a Weibull percentile of 69.3%. The shape factor (β) is determined as 1/slope. The range of β is 2 < β < 4, which leads to better estimations of shelf life. When the test is extended beyond shelf life, most of samples are judged as unacceptable shifting β outside the optimum range [18].


## 2.5. Verifying the FLGT by 2-Alternative Choice

The comparison between fresh green tea and stale green tea at 20°C, 30°C, and 40°C is carried out using the 2-alternative choice analysis based on a published method [20]. A total of 20 undergraduates majored in Tea Science in Anhui Agricultural University (Hefei, Anhui, China) participated in this experiment. These students were 9 males and 11 females and their ages were between 20 and 30 years. These panelists have basic sensory evaluation knowledge and have been trained specifically on distinguishing the fresh and stale odor from green tea. It has been reported that a minimum number of correct judgements to establish significance at various probability levels for paired difference was 15 out of 20 total participants under a significance level of 0.05 [21]. During the analysis, two tea samples are randomly numbered and given to panelists. The panelists are asked to judge whether the two tea samples are both fresh. The FLGT is determined at regular storage temperature by the Q10 method. The green tea samples stored at −20°C and 4°C for 93 days and 150 days, respectively, have been compared in terms of the appearances of the leaves and the infusion colors, aromas, and tastes under a professional panel. These panelists will be asked to give score according to a nine-point scale.


## 2.6. Verifying the FLGT by Chemical Analysis

Contents of tea polyphenols (TPs) were determined by the Chinese National Standard GB/T 8313-2008. Catechins, including C, EC, EGC, GC, ECG, GCG, and EGCG, in the tea leaves were analyzed on an Agilent 1260 HPLC system. A Venusil C18 column (4.6 × 250 mm, 5 μm) was used to separate these compounds (Agilent Technologies, Santa Clara, CA, USA) under a 1.0 mL/min flow rate. Mobile phase consisted of (A) acetonitrile and (B) 1% acetic acid (v/v) in acetonitrile. A gradient program was as follows: 0 to 7 min, 10% to 30% A; 7 to 10 min, 30% A isocratic; 10 to 12 min, 30% to 60% A; 12 to 15 min, 60% to 10% A; and 15 to 20 min, 10% A isocratic. The column was maintained at 40°C during the gradient program. The injection volume was set at 5 μL and the wavelength of the ultraviolet detector was 278 nm. External standard, catechin, was used for the quantitation of catechins in the tea infusion, respectively. All data were repeated three times and recorded as mean ± standard deviation (SD).


## 2.7. Statistical Analysis

Data have been expressed as the mean ± standard deviation (SD) of triplicate tests. The plot between Weibull percentiles and storage time has been drafted using Origin 9.0 software (Origin Lab Corp., Northampton, MA, USA). Heat map was performed using TBtools software (https://github.com/CJ-Chen/TBtools). The 2-alternative choice data have been programmed into minimum numbers of correct judgements to establish significance at various probability levels according to a published method [21].


## 3. Results

### 3.1. Establishment of Nine-Point Scale

A nine-point scale can be established by sensory evaluation (Table2), in which the factor with positive points means no/slight/some/visible difference with fresh tea, and the factor with negative points means entirely/visibly/slightly/some unacceptable as fresh tea. It is suggested that tea sample with a positive weighted score will be considered as fresh tea, whereas the tea with a negative weighted score will be regarded as nonfresh tea. The tea sample with zero weighted score means both acceptable and slightly unacceptable. The higher the number, the greater the acceptability. For example, a score of 4 points was regarded as no difference with fresh tea and a score of −4 points was entirely unacceptable as fresh tea. The overall tea sensory quality, including the appearances of tea leaves and the tea infusion color, aroma, and taste with weight coefficients of 30%, 20%, 30%, and 20%, respectively, has been evaluated by weighted sum method. The next Q10, WHA, and 2-alternative choice analysis are all based on the nine-point scale.Table 2
Establishment of nine-point scale and sensory attributes description.
Score of differenceDifference from fresh teaDescriptionAppearance (30%)Infusion color (20%)Infusion aroma (30%)Infusion taste (20%)4.0No differenceYellowish green, emerald and vividGreen and brightClean and pure, refreshingMellow and fresh3.0Slight differenceYellowish green, emerald and slightly vividGreen and bright, slightly yellowClean and pure, less refreshingMellow and less fresh2.0Some differenceYellowish green, emerald and some brightYellowish green and brightClean and pure with little refreshingMellow with little fresh1.0Visible differenceYellowish green, emerald part turns yellowish greenYellowish greenClean and pureSome thick feeling, without freshness0.0Both acceptable and slightly unacceptableYellowish green, no emeraldYellowish green and the yellow part is lightLittle clean and pureNeutral, just tea taste−1.0Slightly unacceptableYellowish green or greenish yellow, the main color is undistinguishableGreenish yellow and the yellow part is lightUnscentedA little stale taste−2.0Some unacceptableGreenish yellow or yellow, obviouslyGreenish yellowa little dull odor and staleSome stale taste−3.0Visibly unacceptableYellow and a little auburn or dry yellowYellowSome stale odorMore stale taste−4.0Entirely unacceptableAuburn yellowDeep yellowStale odorStale taste


### 3.2. Evaluating the FLGT by theQ10 Method Based on Nine-Point Scale

Table3 lists the score of the attribute factors and the weighted score of these tea samples stored at 20°C, 30°C, and 40°C, respectively. We can observe that the weighted scores of all tea samples decrease during storage and eventually reach negative points, and the higher storage temperature, the less time to reach the negative points. Tea stored at 20°C, 30°C, and 40°C got negative point and lost its freshness at 70 days, 55 days, and 45 days, respectively, which indicates that the high storage temperature can accelerate freshness loss of green tea.Table 3
Sensory attribute score and weighted score of tea samples under the ASLT (20°C, 30°C, and 40°C).
Storage temperature (°C)Time (days)ScoreWeighted scoreLeaves appearanceInfusion colorInfusion aromaInfusion taste2004.00 ± 0.004.00 ± 0.004.00 ± 0.004.00 ± 0.004.0053.50 ± 0.003.77 ± 0.733.50 ± 0.213.50 ± 0.803.00102.58 ± 0.653.50 ± 1.064.00 ± 1.043.50 ± 1.123.37152.30 ± 0.293.30 ± 0.582.40 ± 0.513.00 ± 0.002.67202.40 ± 0.422.87 ± 0.371.75 ± 0.213.50 ± 0.502.52251.80 ± 0.630.80 ± 0.110.30 ± 0.411.50 ± 0.581.09300.00 ± 0.580.00 ± 0.000.67 ± 0.581.83 ± 0.290.57352.20 ± 0.292.80 ± 0.762.00 ± 0.002.20 ± 0.292.26400.70 ± 0.580.60 ± 0.551.30 ± 0.451.05 ± 0.581.0245−1.50 ± 0.80−0.30 ± 0.001.50 ± 0.531.00 ± 0.200.14500.70 ± 0.52−1.00 ± 1.100.40 ± 1.062.00 ± 0.630.53550.70 ± 1.110.30 ± 1.400.30 ± 0.191.60 ± 1.130.68600.42 ± 0.291.40 ± 0.200.00 ± 0.341.40 ± 0.530.6965−0.16 ± 0.680.50 ± 0.220.75 ± 0.371.50 ± 1.110.5870−1.00 ± 0.18−1.00 ± 0.21−0.50 ± 0.65−1.00 ± 0.55−0.883004.00 ± 0.004.00 ± 0.004.00 ± 0.004.00 ± 0.004.0052.00 ± 0.412.80 ± 0.412.80 ± 0.342.30 ± 0.522.46102.71 ± 0.462.20 ± 0.122.57 ± 1.042.68 ± 0.592.56152.80 ± 0.292.00 ± 1.003.00 ± 0.582.00 ± 1.002.54202.40 ± 0.552.50 ± 0.502.30 ± 0.742.60 ± 0.552.43251.50 ± 0.342.00 ± 0.390.16 ± 0.552.50 ± 1.201.40301.00 ± 0.190.00 ± 0.111.00 ± 0.491.00 ± 1.170.80351.00 ± 0.501.00 ± 0.351.00 ± 0.001.00 ± 0.901.00400.80 ± 1.00−0.30 ± 0.08−1.00 ± 0.29−2.00 ± 0.300.1045−1.30 ± 0.531.00 ± 0.000.00 ± 0.151.00 ± 0.000.0150−0.50 ± 0.611.00 ± 0.460.00 ± 0.142.00 ± 0.160.2555−1.30 ± 0.39−1.60 ± 0.60−1.00 ± 0.71−0.67 ± 0.51−1.1460−0.71 ± 0.76−1.00 ± 0.58−1.67 ± 0.520.14 ± 0.69−0.5965−1.00 ± 0.75−0.60 ± 0.75−2.00 ± 0.390.00 ± 0.33−0.6270−1.60 ± 0.55−2.30 ± 0.29−2.20 ± 0.34−0.20 ± 0.84−1.644004.00 ± 0.004.00 ± 0.004.00 ± 0.004.00 ± 0.004.0052.50 ± 0.552.30 ± 0.522.75 ± 0.672.25 ± 0.322.49102.60 ± 0.763.25 ± 0.362.20 ± 0.571.80 ± 0.192.45151.00 ± 0.002.00 ± 0.001.00 ± 0.562.00 ± 0.001.40201.50 ± 0.501.25 ± 0.391.00 ± 0.451.60 ± 0.551.32250.40 ± 0.67−0.25 ± 0.160.00 ± 0.000.00 ± 0.600.07300.50 ± 0.160.30 ± 0.25−1.00 ± 0.291.00 ± 0.500.11350.75 ± 0.501.00 ± 0.581.30 ± 0.412.00 ± 0.581.22400.00 ± 0.29−0.25 ± 0.230.00 ± 0.570.80 ± 0.000.1145−2.00 ± 0.15−0.67 ± 1.00−1.67 ± 0.66−0.50 ± 0.75−1.3450−1.70 ± 0.28−2.17 ± 0.18−1.80 ± 0.170.40 ± 0.75−1.4055−1.90 ± 0.19−1.90 ± 0.90−2.60 ± 0.60−0.85 ± 0.35−1.9060−2.00 ± 0.58−1.40 ± 0.31−1.60 ± 0.83−0.71 ± 0.65−1.5065−1.80 ± 0.17−1.30 ± 0.32−1.30 ± 0.39−1.00 ± 0.27−1.3970−2.40 ± 0.30−0.67 ± 0.45−1.75 ± 1.14−1.00 ± 0.34−1.58Data were the mean ± standard deviation of triplicate tests for leaves appearance, as well as infusion color, aroma, and taste.It is found that the FLGTs are 55 days and 45 days at 30°C and 40°C, respectively (Table3), which can infer that Q10 of the green tea samples is 11/9 by equation in Methods. Additionally, the FLGT stored at 20°C can be further calculated using the following equation:(6)θ20°C=θ30°C×Q30−20/10.In the above equation, θ30°C is 55 days and Q30−20/10 is 11/9. The FLGT stored at 20°C can be obtained as 67 days according to the Q10 method. In our sensory evaluation result, the weighted score was 0.58 at 65 days and was −0.88 at 70 days, which means that the FLGT stored at 20°C is between 65 and 70 days. The FLGT obtained by the Q10 method agrees well with that of sensory evaluation at 20°C. The tea after the FLGT exhibits a yellowish leaves appearance and its infusion is obviously yellow color with strong stale aroma and nonfresh taste according to our panelists.


### 3.3. Evaluating the FLGT by WHA

In the WHA, the panelists will be asked to judge whether the tea sample after different storage periods will be still considered acceptable as fresh tea in terms of the overall quality of tea (Table4). The overall quality of the tea samples was identified as “not accepted” when more than half of the panelists marked a “✕.” Table 1 shows Weibull hazard ranking for tea samples stored at an accelerated shelf-life condition (20°C, 30°C, and 40°C). According to the WHA, the betas (β) at three temperatures are 3.37, 3.13, and 3.42, respectively, which meet the requirements of Weibull distribution for β and indicate that Figure 1 can accurately predict the FLGT at three temperatures. Probability Weibull plot of time is shown on Figure 1. The FLGT is the time when the Weibull percentile reached 69.3% [18]. The FLGTs are 67 days, 57 days, and 42 days for the samples stored at 20°C, 30°C, and 40°C, respectively. Evaluations of the FLGTs by WHA are almost well consistent with the results from the sensory evaluation and the Q10 method.Table 4
Acceptability test of tea samples under the ASLT (20°C, 30°C, and 40°C).
Storage temperature (°C)Time (days)Individual descriptionOverall description205✓✓Accept10✓✓✓Accept15✓✓✓✓Accept20✓✓✓✓✓Accept25✓✓✓✓✓✓Accept30✓✓✓✓✓✓✓Accept35✓✓✓✓✓✓✓✓Accept40✓✓✓✓✓✓✓✓✓Accept45✓✓✓✓✓✓✓✓✓✓Accept50✓✓✓✓✓✓✓✓✕11✕12Accept55✓✓✓✓✓✓✓✓✓✓Accept60✓✓✓✓✓✓✓✓✓✕10Accept65✓✓✓✓✓✓✓✕7✕8✕9Accept70✓✓✓✓✕1✕2✕3✕4✕5✕6Reject305✓✓Accept10✓✓✓Accept15✓✓✓✓Accept20✓✓✓✓✓Accept25✓✓✓✓✓✓Accept30✓✓✓✓✓✓✓Accept35✓✓✓✓✓✓✓✓Accept40✓✓✓✓✓✓✓✕16✕17Accept45✓✓✓✓✓✓✓✓✕14✕15Accept50✓✓✓✓✓✕9✕10✕11✕12✕13Accept55✓✓✕1✕2✕3✕4✕5✕6✕7✕8Reject405✓✓Accept10✓✓✓Accept15✓✓✓✓Accept20✓✓✓✓✓Accept25✓✓✓✓✕16✕17Accept30✓✓✓✓✓✓✓✕14✕15Accept35✓✓✓✓✓✓✓✓✕12✕13Accept40✓✓✓✓✓✓✓✕9✕10✕11Accept45✓✓✕1✕2✕3✕4✕5✕6✕7✕8RejectA “✓” was checked when the panelists accepted the overall quality of the tea, whereas the tea with its overall quality not accepted by the panelists was given a “✕” in the analysis.Figure 1
Probability Weibull plot of time. (a) Tea samples were stored at 20°C; (b) tea samples were stored at 30°C; (c) tea samples were stored at 40°C.
(a)(b)(c)


### 3.4. Verifying the FLGT by 2-Alternative Choice

We find that a total of 17 panelists can distinguish the freshness difference between the fresh tea sample and the tea sample stored at 40°C for 45 days. Meanwhile, a difference on the freshness between the fresh tea sample and the tea stored at 30°C for 55 days has been also confirmed by 18 panelists. 16 panelists gave a different judgement between the fresh tea and the tea stored at 20°C for 67 days. All the three numbers are greater than 15; therefore, all these stored tea samples exhibit the significant difference compared to the fresh tea sample in terms of their freshness. The analysis of the FLGT using 2-alternative choice approach further verifies the results of the tea samples under the ASLT. This also indicates that the alterations in the quality attributes (leaves appearance, as well as tea infusion color, aroma, and taste) of the tea sample under the ASLT can provide reliable information on the investigation of freshness loss of green tea under a regular temperature condition.


### 3.5. Verifying the FLGT by Chemical Analysis

To further verify the correctness of the sensory results, we tested the content of tea polyphenols and catechins, including C, EC, EGC, GC, ECG, GCG, and EGCG, in the tea samples at each stage of the ASLT. The results showed that the polyphenols and catechins in the tea samples had some changes during the ASLT. Contents of GA, EGC, GCG, and ECG decreased, and contents of TPs, EC, and EGCG first increased and then decreased, while C content increased during the ASLT. Furthermore, the tea samples could be completely divided into two categories by hierarchical cluster analysis (Figure2). The same batch of tea samples was sampled at intervals. In the early stage of storage, although the temperature was different, the compounds content in the tea samples changed little, and they were clustered into one category, which was fresh tea group (blue box in Figure 2). At the same time, the remaining samples were clustered into another category called stale tea group, in which it was worth noting that the four tea samples at 20°C for 67 days, 20°C for 65 days, 30°C for 55 days, and 40°C for 45 days were clustered together alone (red box in Figure 2), which were consistent with the results of FLGT by sensory evaluation.Figure 2
Hierarchical cluster analysis (HCA) for tea samples under the ASLT (20°C, 30°C, and 40°C).


### 3.6. Evaluating the FLGT at Regular Storage Temperature by theQ10 Method

According to the theory of theQ10 method, Q10 of these tea samples in this study is 11/9. Therefore, the freshness duration time for the tea sample stored at 4°C and −20°C can be theoretically predicted to be 93 days and 150 days, respectively, as shown in Table 5.Table 5
Sensory evaluation and acceptability test of FLGT stored at 4°C for 93 days and at −20°C for 150 days.
Store temperature (°C)Time (days)ScoreWeighted scoreAcceptabilityLeaves appearanceInfusion colorInfusion aromaInfusion taste4932.00 ± 0.351.00 ± 0.00−2.00 ± 0.90−1.00 ± 0.25−0.18Reject−201503.50 ± 0.454.00 ± 0.193.00 ± 0.002.75 ± 0.333.30AcceptData were the mean ± standard deviation of triplicate tests for leaves appearance, as well as infusion color, aroma, and taste.In order to verify the evaluated freshness duration for the tea samples stored at 4°C and −20°C, a sensory evaluation has been also carried out using the tea samples stored at 4°C for 93 days and the tea samples stored at −20°C for 150 days with 6 professional panelists (Table5). Appearance of the leaves and infusion color of tea stored at 4°C for 93 days exhibit some difference/visible difference with the fresh tea. However, the aroma and taste exhibit mean with some unacceptable/slightly unacceptable appearances and get a negative overall weighted score (Table 5). The panelists describe the quality of green tea stored at 4°C for 93 days as aged and stale and the tea gets “Reject” in the acceptability test, which also verifies that the method is reliable to evaluate the FLGT by the Q10 method.The appearances of the leaves and the color, aroma, and taste of the tea stored at −20°C for 150 days get positive scores, with no or slight difference compared to the fresh tea (Table5), and the panelists describe the quality of tea as almost fresh, which means that the tea samples stored at −20°C for 150 days also remain fresh and do not reach the FLGT.


## 3.1. Establishment of Nine-Point Scale

A nine-point scale can be established by sensory evaluation (Table2), in which the factor with positive points means no/slight/some/visible difference with fresh tea, and the factor with negative points means entirely/visibly/slightly/some unacceptable as fresh tea. It is suggested that tea sample with a positive weighted score will be considered as fresh tea, whereas the tea with a negative weighted score will be regarded as nonfresh tea. The tea sample with zero weighted score means both acceptable and slightly unacceptable. The higher the number, the greater the acceptability. For example, a score of 4 points was regarded as no difference with fresh tea and a score of −4 points was entirely unacceptable as fresh tea. The overall tea sensory quality, including the appearances of tea leaves and the tea infusion color, aroma, and taste with weight coefficients of 30%, 20%, 30%, and 20%, respectively, has been evaluated by weighted sum method. The next Q10, WHA, and 2-alternative choice analysis are all based on the nine-point scale.Table 2
Establishment of nine-point scale and sensory attributes description.
Score of differenceDifference from fresh teaDescriptionAppearance (30%)Infusion color (20%)Infusion aroma (30%)Infusion taste (20%)4.0No differenceYellowish green, emerald and vividGreen and brightClean and pure, refreshingMellow and fresh3.0Slight differenceYellowish green, emerald and slightly vividGreen and bright, slightly yellowClean and pure, less refreshingMellow and less fresh2.0Some differenceYellowish green, emerald and some brightYellowish green and brightClean and pure with little refreshingMellow with little fresh1.0Visible differenceYellowish green, emerald part turns yellowish greenYellowish greenClean and pureSome thick feeling, without freshness0.0Both acceptable and slightly unacceptableYellowish green, no emeraldYellowish green and the yellow part is lightLittle clean and pureNeutral, just tea taste−1.0Slightly unacceptableYellowish green or greenish yellow, the main color is undistinguishableGreenish yellow and the yellow part is lightUnscentedA little stale taste−2.0Some unacceptableGreenish yellow or yellow, obviouslyGreenish yellowa little dull odor and staleSome stale taste−3.0Visibly unacceptableYellow and a little auburn or dry yellowYellowSome stale odorMore stale taste−4.0Entirely unacceptableAuburn yellowDeep yellowStale odorStale taste


## 3.2. Evaluating the FLGT by theQ10 Method Based on Nine-Point Scale

Table3 lists the score of the attribute factors and the weighted score of these tea samples stored at 20°C, 30°C, and 40°C, respectively. We can observe that the weighted scores of all tea samples decrease during storage and eventually reach negative points, and the higher storage temperature, the less time to reach the negative points. Tea stored at 20°C, 30°C, and 40°C got negative point and lost its freshness at 70 days, 55 days, and 45 days, respectively, which indicates that the high storage temperature can accelerate freshness loss of green tea.Table 3
Sensory attribute score and weighted score of tea samples under the ASLT (20°C, 30°C, and 40°C).
Storage temperature (°C)Time (days)ScoreWeighted scoreLeaves appearanceInfusion colorInfusion aromaInfusion taste2004.00 ± 0.004.00 ± 0.004.00 ± 0.004.00 ± 0.004.0053.50 ± 0.003.77 ± 0.733.50 ± 0.213.50 ± 0.803.00102.58 ± 0.653.50 ± 1.064.00 ± 1.043.50 ± 1.123.37152.30 ± 0.293.30 ± 0.582.40 ± 0.513.00 ± 0.002.67202.40 ± 0.422.87 ± 0.371.75 ± 0.213.50 ± 0.502.52251.80 ± 0.630.80 ± 0.110.30 ± 0.411.50 ± 0.581.09300.00 ± 0.580.00 ± 0.000.67 ± 0.581.83 ± 0.290.57352.20 ± 0.292.80 ± 0.762.00 ± 0.002.20 ± 0.292.26400.70 ± 0.580.60 ± 0.551.30 ± 0.451.05 ± 0.581.0245−1.50 ± 0.80−0.30 ± 0.001.50 ± 0.531.00 ± 0.200.14500.70 ± 0.52−1.00 ± 1.100.40 ± 1.062.00 ± 0.630.53550.70 ± 1.110.30 ± 1.400.30 ± 0.191.60 ± 1.130.68600.42 ± 0.291.40 ± 0.200.00 ± 0.341.40 ± 0.530.6965−0.16 ± 0.680.50 ± 0.220.75 ± 0.371.50 ± 1.110.5870−1.00 ± 0.18−1.00 ± 0.21−0.50 ± 0.65−1.00 ± 0.55−0.883004.00 ± 0.004.00 ± 0.004.00 ± 0.004.00 ± 0.004.0052.00 ± 0.412.80 ± 0.412.80 ± 0.342.30 ± 0.522.46102.71 ± 0.462.20 ± 0.122.57 ± 1.042.68 ± 0.592.56152.80 ± 0.292.00 ± 1.003.00 ± 0.582.00 ± 1.002.54202.40 ± 0.552.50 ± 0.502.30 ± 0.742.60 ± 0.552.43251.50 ± 0.342.00 ± 0.390.16 ± 0.552.50 ± 1.201.40301.00 ± 0.190.00 ± 0.111.00 ± 0.491.00 ± 1.170.80351.00 ± 0.501.00 ± 0.351.00 ± 0.001.00 ± 0.901.00400.80 ± 1.00−0.30 ± 0.08−1.00 ± 0.29−2.00 ± 0.300.1045−1.30 ± 0.531.00 ± 0.000.00 ± 0.151.00 ± 0.000.0150−0.50 ± 0.611.00 ± 0.460.00 ± 0.142.00 ± 0.160.2555−1.30 ± 0.39−1.60 ± 0.60−1.00 ± 0.71−0.67 ± 0.51−1.1460−0.71 ± 0.76−1.00 ± 0.58−1.67 ± 0.520.14 ± 0.69−0.5965−1.00 ± 0.75−0.60 ± 0.75−2.00 ± 0.390.00 ± 0.33−0.6270−1.60 ± 0.55−2.30 ± 0.29−2.20 ± 0.34−0.20 ± 0.84−1.644004.00 ± 0.004.00 ± 0.004.00 ± 0.004.00 ± 0.004.0052.50 ± 0.552.30 ± 0.522.75 ± 0.672.25 ± 0.322.49102.60 ± 0.763.25 ± 0.362.20 ± 0.571.80 ± 0.192.45151.00 ± 0.002.00 ± 0.001.00 ± 0.562.00 ± 0.001.40201.50 ± 0.501.25 ± 0.391.00 ± 0.451.60 ± 0.551.32250.40 ± 0.67−0.25 ± 0.160.00 ± 0.000.00 ± 0.600.07300.50 ± 0.160.30 ± 0.25−1.00 ± 0.291.00 ± 0.500.11350.75 ± 0.501.00 ± 0.581.30 ± 0.412.00 ± 0.581.22400.00 ± 0.29−0.25 ± 0.230.00 ± 0.570.80 ± 0.000.1145−2.00 ± 0.15−0.67 ± 1.00−1.67 ± 0.66−0.50 ± 0.75−1.3450−1.70 ± 0.28−2.17 ± 0.18−1.80 ± 0.170.40 ± 0.75−1.4055−1.90 ± 0.19−1.90 ± 0.90−2.60 ± 0.60−0.85 ± 0.35−1.9060−2.00 ± 0.58−1.40 ± 0.31−1.60 ± 0.83−0.71 ± 0.65−1.5065−1.80 ± 0.17−1.30 ± 0.32−1.30 ± 0.39−1.00 ± 0.27−1.3970−2.40 ± 0.30−0.67 ± 0.45−1.75 ± 1.14−1.00 ± 0.34−1.58Data were the mean ± standard deviation of triplicate tests for leaves appearance, as well as infusion color, aroma, and taste.It is found that the FLGTs are 55 days and 45 days at 30°C and 40°C, respectively (Table3), which can infer that Q10 of the green tea samples is 11/9 by equation in Methods. Additionally, the FLGT stored at 20°C can be further calculated using the following equation:(6)θ20°C=θ30°C×Q30−20/10.In the above equation, θ30°C is 55 days and Q30−20/10 is 11/9. The FLGT stored at 20°C can be obtained as 67 days according to the Q10 method. In our sensory evaluation result, the weighted score was 0.58 at 65 days and was −0.88 at 70 days, which means that the FLGT stored at 20°C is between 65 and 70 days. The FLGT obtained by the Q10 method agrees well with that of sensory evaluation at 20°C. The tea after the FLGT exhibits a yellowish leaves appearance and its infusion is obviously yellow color with strong stale aroma and nonfresh taste according to our panelists.


## 3.3. Evaluating the FLGT by WHA

In the WHA, the panelists will be asked to judge whether the tea sample after different storage periods will be still considered acceptable as fresh tea in terms of the overall quality of tea (Table4). The overall quality of the tea samples was identified as “not accepted” when more than half of the panelists marked a “✕.” Table 1 shows Weibull hazard ranking for tea samples stored at an accelerated shelf-life condition (20°C, 30°C, and 40°C). According to the WHA, the betas (β) at three temperatures are 3.37, 3.13, and 3.42, respectively, which meet the requirements of Weibull distribution for β and indicate that Figure 1 can accurately predict the FLGT at three temperatures. Probability Weibull plot of time is shown on Figure 1. The FLGT is the time when the Weibull percentile reached 69.3% [18]. The FLGTs are 67 days, 57 days, and 42 days for the samples stored at 20°C, 30°C, and 40°C, respectively. Evaluations of the FLGTs by WHA are almost well consistent with the results from the sensory evaluation and the Q10 method.Table 4
Acceptability test of tea samples under the ASLT (20°C, 30°C, and 40°C).
Storage temperature (°C)Time (days)Individual descriptionOverall description205✓✓Accept10✓✓✓Accept15✓✓✓✓Accept20✓✓✓✓✓Accept25✓✓✓✓✓✓Accept30✓✓✓✓✓✓✓Accept35✓✓✓✓✓✓✓✓Accept40✓✓✓✓✓✓✓✓✓Accept45✓✓✓✓✓✓✓✓✓✓Accept50✓✓✓✓✓✓✓✓✕11✕12Accept55✓✓✓✓✓✓✓✓✓✓Accept60✓✓✓✓✓✓✓✓✓✕10Accept65✓✓✓✓✓✓✓✕7✕8✕9Accept70✓✓✓✓✕1✕2✕3✕4✕5✕6Reject305✓✓Accept10✓✓✓Accept15✓✓✓✓Accept20✓✓✓✓✓Accept25✓✓✓✓✓✓Accept30✓✓✓✓✓✓✓Accept35✓✓✓✓✓✓✓✓Accept40✓✓✓✓✓✓✓✕16✕17Accept45✓✓✓✓✓✓✓✓✕14✕15Accept50✓✓✓✓✓✕9✕10✕11✕12✕13Accept55✓✓✕1✕2✕3✕4✕5✕6✕7✕8Reject405✓✓Accept10✓✓✓Accept15✓✓✓✓Accept20✓✓✓✓✓Accept25✓✓✓✓✕16✕17Accept30✓✓✓✓✓✓✓✕14✕15Accept35✓✓✓✓✓✓✓✓✕12✕13Accept40✓✓✓✓✓✓✓✕9✕10✕11Accept45✓✓✕1✕2✕3✕4✕5✕6✕7✕8RejectA “✓” was checked when the panelists accepted the overall quality of the tea, whereas the tea with its overall quality not accepted by the panelists was given a “✕” in the analysis.Figure 1
Probability Weibull plot of time. (a) Tea samples were stored at 20°C; (b) tea samples were stored at 30°C; (c) tea samples were stored at 40°C.
(a)(b)(c)


## 3.4. Verifying the FLGT by 2-Alternative Choice

We find that a total of 17 panelists can distinguish the freshness difference between the fresh tea sample and the tea sample stored at 40°C for 45 days. Meanwhile, a difference on the freshness between the fresh tea sample and the tea stored at 30°C for 55 days has been also confirmed by 18 panelists. 16 panelists gave a different judgement between the fresh tea and the tea stored at 20°C for 67 days. All the three numbers are greater than 15; therefore, all these stored tea samples exhibit the significant difference compared to the fresh tea sample in terms of their freshness. The analysis of the FLGT using 2-alternative choice approach further verifies the results of the tea samples under the ASLT. This also indicates that the alterations in the quality attributes (leaves appearance, as well as tea infusion color, aroma, and taste) of the tea sample under the ASLT can provide reliable information on the investigation of freshness loss of green tea under a regular temperature condition.


## 3.5. Verifying the FLGT by Chemical Analysis

To further verify the correctness of the sensory results, we tested the content of tea polyphenols and catechins, including C, EC, EGC, GC, ECG, GCG, and EGCG, in the tea samples at each stage of the ASLT. The results showed that the polyphenols and catechins in the tea samples had some changes during the ASLT. Contents of GA, EGC, GCG, and ECG decreased, and contents of TPs, EC, and EGCG first increased and then decreased, while C content increased during the ASLT. Furthermore, the tea samples could be completely divided into two categories by hierarchical cluster analysis (Figure2). The same batch of tea samples was sampled at intervals. In the early stage of storage, although the temperature was different, the compounds content in the tea samples changed little, and they were clustered into one category, which was fresh tea group (blue box in Figure 2). At the same time, the remaining samples were clustered into another category called stale tea group, in which it was worth noting that the four tea samples at 20°C for 67 days, 20°C for 65 days, 30°C for 55 days, and 40°C for 45 days were clustered together alone (red box in Figure 2), which were consistent with the results of FLGT by sensory evaluation.Figure 2
Hierarchical cluster analysis (HCA) for tea samples under the ASLT (20°C, 30°C, and 40°C).


## 3.6. Evaluating the FLGT at Regular Storage Temperature by theQ10 Method

According to the theory of theQ10 method, Q10 of these tea samples in this study is 11/9. Therefore, the freshness duration time for the tea sample stored at 4°C and −20°C can be theoretically predicted to be 93 days and 150 days, respectively, as shown in Table 5.Table 5
Sensory evaluation and acceptability test of FLGT stored at 4°C for 93 days and at −20°C for 150 days.
Store temperature (°C)Time (days)ScoreWeighted scoreAcceptabilityLeaves appearanceInfusion colorInfusion aromaInfusion taste4932.00 ± 0.351.00 ± 0.00−2.00 ± 0.90−1.00 ± 0.25−0.18Reject−201503.50 ± 0.454.00 ± 0.193.00 ± 0.002.75 ± 0.333.30AcceptData were the mean ± standard deviation of triplicate tests for leaves appearance, as well as infusion color, aroma, and taste.In order to verify the evaluated freshness duration for the tea samples stored at 4°C and −20°C, a sensory evaluation has been also carried out using the tea samples stored at 4°C for 93 days and the tea samples stored at −20°C for 150 days with 6 professional panelists (Table5). Appearance of the leaves and infusion color of tea stored at 4°C for 93 days exhibit some difference/visible difference with the fresh tea. However, the aroma and taste exhibit mean with some unacceptable/slightly unacceptable appearances and get a negative overall weighted score (Table 5). The panelists describe the quality of green tea stored at 4°C for 93 days as aged and stale and the tea gets “Reject” in the acceptability test, which also verifies that the method is reliable to evaluate the FLGT by the Q10 method.The appearances of the leaves and the color, aroma, and taste of the tea stored at −20°C for 150 days get positive scores, with no or slight difference compared to the fresh tea (Table5), and the panelists describe the quality of tea as almost fresh, which means that the tea samples stored at −20°C for 150 days also remain fresh and do not reach the FLGT.


## 4. Conclusions

The suitable drinking period of green tea is relatively short, so it is important to explore the FLGT. In this paper, the FLGTs of tea samples stored at 20°C, 30°C, and 40°C have been evaluated to be 67 days, 55 days, and 45 days using theQ10 method and WHA in ASLT based on nine-point scale sensory evaluation and acceptability test session. The results have also been verified by 2-alternative choice analysis and chemical analysis. The FLGT of the tea samples stored at 4°C was predicted to be 93 days using the Q10 method, and the FLGT of the tea samples stored at 4°C has also been verified by acceptability test. Therefore, this paper can predict the suitable drinking period of green tea stored at a certain temperature, provide a theoretic instruction to keep fresh green tea, and suggest that researchers should conduct their experiments before the FLGT.



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*Source: 2955839-2022-01-13.xml*