# Experimental Study on the Fine-Grained Uranium Tailings Reinforced by MICP

Authors: Rong Gui; Yu-xiang Pan; De-xin Ding; Yong Liu; Zhi-jun Zhang
Journal: Advances in Civil Engineering
 (2018)

---

## Abstract

Sporosarcina Pasteurii was chosen for the experiment to study the effect and mechanism of fine-grained uranium tailings reinforced by MICP. The biochemical characteristics of strains and microbial immobilization in uranium tailings were analyzed. The results showed that the CaCO3 production rate is positively correlated with the physiological activity of Sporosarcina Pasteurii and the concentration of calcium sources, and the higher the solution concentration of CaCl2, the lower the discharge rate of bacterial liquid from the sand column, but high concentration of CaCl2 solution will affect the uniform distribution and migration of bacteria in the uranium tailings. After 16 days, the direct shear was used to test the reinforcement effects of fine-grained uranium tailings by MICP. The cohesive force and the internal friction angle of fine-grained uranium tailings were increased by 140.1% and 46.7%. The production amount of CaCO3 is 138.89 kg/m3. The results showed that the MICP-reinforced technology can effectively improve the shear strength of the uranium tailings, and the experiment provides a new method for the reinforcement of the fine-grained uranium tailings dam.


---

## Body

## 1. Introduction

With the rapid development of nuclear industry in China, the demand for nuclear fuel increased rapidly. However, a large amount of radioactive waste was produced during the mining and metallurgy process of uranium, and many tailings reservoirs have been built to store them. As a special hydraulic structure, the safety of tailings reservoir has always been the focus of social attention. However, there were numerous serious accidents caused by failure of tailings dam at home and abroad for various reasons [1, 2]. On September 8, 2008, a catastrophic failure accident occurred in the tailings reservoir of Xinta Mining Ltd. Co. Xiangfen County, Shanxi Province, China, that killed 281 people and claim a direct economic loss of 96.91 million yuan. On July 19, 1985, a fluorite tailings dam of Prealpi Mineraia failed at Stava, Trento, Italy. 200,000 m3 of tailings flowed 4.2 km downstream at a speed of up to 90 km/h that killed 268 people and destroyed 62 buildings. However, due to the uranium tailings contain radionuclide such as uranium and radium, the catastrophe caused by failure of uranium tailings dam could be even more serious than other tailings dam, which also will cause severe radioactive pollution to the surrounding water and soil. Therefore, in order to ensure the stability of the uranium tailings dam, it is necessary to study the reinforcement technology of uranium tailings.Traditional soil reinforcement techniques use sodium silicate, cement, and other chemical pulp as grouting materials which have some obstacles such as high cost, high-energy consumption, and permanent soil pollution [3–5]. Whiffin was first proposed to apply microbial diagenesis to cement the loose sand particles and achieved good effect in 2004 [6]. This has led to a research boom in the reinforcement of rock and soil by MICP technology. Paassen et al. applied the MICP technology to reinforce 100 m3 in situ sand base, and the unconfined compressive strength and stiffness of the treated sand base have a significant improvement [7]. Paassen applied the MICP technology to reinforce the 3–20 m gravel layer under the surface in the Netherlands, and no collapse happened in the reinforced gravel layer in the horizontal directional drilling and the gas pipeline laying [8]. Burbank et al. use the indigenous diagenetic microorganisms to reinforce the soil in the laboratory. The results show that calcium carbonate production in the soil was 20–48 kg/m3, and the static penetration value of the reinforced soil increased by 2.2 times [9]. Soon et al. [10] applied the MICP technology to reinforce the low-permeability soils and proved that the technology can also improve the mechanical properties of clay or silt. Canakci et al. applied the MICP technology to treat the peat soils with the low-strength and high-compression. After treatment, the shear strength and erosion resistance of reinforced peat soils increased, and calcium carbonate production in peat soil is 16% of the weight of the soil [11]. Mahawish et al. studied the feasibility of applying MICP technology to improve the mechanical properties of coarse sand and applying MICP technology to reinforce the gravel piles, sand piles, and bauxite columns. The uniaxial compressive strength of reinforced piles was up to 8.9 MPa–2.3 GPa [12]. Pusadkar et al. reinforced the sand slop in the laboratory by injecting bacteria (S. Pasteurii) and cement solution in sand, and the bearing capacity of slope footing increased significantly after MICP treatment [13]. Grabiec et al. applied the MICP technology to reinforce the incompletely compacted silty clays, and found that MICP technology can make diagenesis in the silty clays and significantly improve the soil stiffness [14].The biological reinforcement technology aim at improving soil mechanical properties, and erosion resistance precipitate calcium carbonate crystals by the microbial biochemical activities. This technology is often referred to as microbial-induced calcite precipitation (MICP) [15]. At present, MICP technology has been successfully applied to solve geotechnical engineering problems, such as strengthening the bearing capacity of foundation soil, reducing the liquefaction of soils caused by earthquakes, reducing the swelling potential of foundations and roads, and reducing the permeability of tunnel walls and soils [7, 16–20]. These researches have shown that the MICP technology has the advantages over the traditional reinforcement technology which not only reduced the disturbance of chemical grouting reinforcement but also has the advantages of economy, the environmental protection, and large curing radius.Because the uranium tailings contain radionuclides and have different particle gradations from other porous media, so it is necessary to verify the feasibility of using the MICP technology to reinforce the fine-grained uranium tailings. Therefore, it is necessary to verify the feasibility of using the MICP technology to reinforce the fine-grained uranium tailings.


## 2. Materials and Methods

### 2.1. Strains and Culture Medium

TheSporosarcina Pasteurii used in the experiment was from the China General Microbiological Culture Collection Center (No. ATCC 11859). The components of culture medium are shown in Table 1. Firstly, the urea solution was filtered by a steel sterilizing filter with 0.45 μm and 0.22 μm microporous permeable membranes. The urea solution was prepared separately during the sterilization because the urea is easy to decompose under high temperature. Then, the rest of the components were sterilized by pressurized steam sterilization at 121°C for 20 minutes. After preparation, the two solutions were mixed in proportion to get the required solution.Table 1
Composition of culture medium used in the experiment.
Medium name
pH
Components
CASO liquid medium
7.3
Casein 15 g, soy peptone 5 g, sodium chloride 5 g, urea 20 g, nickel chloride 0.0013 g, deionized water 1000 mL
CASO solid medium
7.3
Casein 15 g, soy peptone 5 g, sodium chloride 5 g, urea 20 g, nickel chloride 0.0013 g, 20 g agar powder, deionized water 1000 mL
Mixed medium
7.3
Casein 15 g, soy peptone 5 g, sodium chloride 5 g, urea 30 g (0.5 mol/L), nickel chloride 0.0013 g, calcium chloride 55.5 g (0.5 mol/L), deionized water 1000 mL


### 2.2. Uranium Tailings Samples

The samples were from a uranium tailings reservoir in south China. The screening test showed that the gradation parametersd10 of the samples is 0.067 mm, d30 is 0.117 mm, d60 is 0.208 mm, the nonuniform coefficient Cu is 2.663, the curvature coefficient Cc is 0.893, the relative density GS is 2.67, the density ρ is 1.454 g/cm3, and the void ratio e is 0.752.


### 2.3. Fixative Solution and Cement Solution

The bacterial liquid is easily discharged from uranium tailings because theSporosarcina Pasteurii has a diameter size within 0.5–3.0 μm. In order to reduce the discharge rate (the ratio of the OD600 value of the discharged bacterial liquid to the OD600 value of the inoculated bacterial liquid) of bacterial liquid from the sand column, CaCl2 was chosen as the fixative solution. According to related references [9, 13], the higher the concentration of the fixative solution was, the lower the discharge rate of the bacterial liquid was. But under a certain concentration of CaCl2, the inoculated bacterial liquid will form the floccules, and its diameter sizes enlarged with the increasing concentration of CaCl2 and blocked the pores between the uranium tailings, resulting in nonuniform distribution of bacterial liquid and unstable transmission of cement solution in the sand column. It indicated that the fixation and uniform distribution of the bacterial liquid in the sand column cannot simultaneously reach their optimum value, especially to different sand samples. So it is necessary to conduct the experimental analysis according to actual needs.Therefore, in order to study the effects of different concentrations of the fixative solution on the fixation and migration ofSporosarcina Pasteurii in uranium tailings, five different concentrations of CaCl2 solution (0.005 mol/L, 0.015 mol/L, 0.025 mol/L, 0.035 mol/L, and 0.045 mol/L) were set to in the experiment. Based on the experimental results, the cement solution was determined with 0.5 mol/L urea and 0.5 mol/L CaCl2 solution (equivalent volume mixture).


### 2.4. Physical Model

The physical model of the sand columns were constructed of polyvinyl chloride (PVC) 7 cm height with an inner diameter of 6.18 cm (the same as the diameter size of the sand samples in the direct shear test). The upper part of the experimental device have a grouting port connected to the sealed plastic bottle with a rubber hose and a reserved vent. The grouting pipe and the outlet pipe have a rubber pipe with a water stop valve. In order to prevent the sand from entering the grouting pipe and outlet pipe, two gauze layers were set on both sides of the sand columns. And, two gravel layers (particle size 2 ± 0.5 mm) were laid on both sides of the sand column to avoid scouring the uranium tailings and clogging of the grouting mouth during the experiment. The experimental device is shown in Figure1 and Figure 2.Figure 1
Schematic diagram of the MICP experimental device.Figure 2
Physical model of the MICP experimental device.


## 2.1. Strains and Culture Medium

TheSporosarcina Pasteurii used in the experiment was from the China General Microbiological Culture Collection Center (No. ATCC 11859). The components of culture medium are shown in Table 1. Firstly, the urea solution was filtered by a steel sterilizing filter with 0.45 μm and 0.22 μm microporous permeable membranes. The urea solution was prepared separately during the sterilization because the urea is easy to decompose under high temperature. Then, the rest of the components were sterilized by pressurized steam sterilization at 121°C for 20 minutes. After preparation, the two solutions were mixed in proportion to get the required solution.Table 1
Composition of culture medium used in the experiment.
Medium name
pH
Components
CASO liquid medium
7.3
Casein 15 g, soy peptone 5 g, sodium chloride 5 g, urea 20 g, nickel chloride 0.0013 g, deionized water 1000 mL
CASO solid medium
7.3
Casein 15 g, soy peptone 5 g, sodium chloride 5 g, urea 20 g, nickel chloride 0.0013 g, 20 g agar powder, deionized water 1000 mL
Mixed medium
7.3
Casein 15 g, soy peptone 5 g, sodium chloride 5 g, urea 30 g (0.5 mol/L), nickel chloride 0.0013 g, calcium chloride 55.5 g (0.5 mol/L), deionized water 1000 mL


## 2.2. Uranium Tailings Samples

The samples were from a uranium tailings reservoir in south China. The screening test showed that the gradation parametersd10 of the samples is 0.067 mm, d30 is 0.117 mm, d60 is 0.208 mm, the nonuniform coefficient Cu is 2.663, the curvature coefficient Cc is 0.893, the relative density GS is 2.67, the density ρ is 1.454 g/cm3, and the void ratio e is 0.752.


## 2.3. Fixative Solution and Cement Solution

The bacterial liquid is easily discharged from uranium tailings because theSporosarcina Pasteurii has a diameter size within 0.5–3.0 μm. In order to reduce the discharge rate (the ratio of the OD600 value of the discharged bacterial liquid to the OD600 value of the inoculated bacterial liquid) of bacterial liquid from the sand column, CaCl2 was chosen as the fixative solution. According to related references [9, 13], the higher the concentration of the fixative solution was, the lower the discharge rate of the bacterial liquid was. But under a certain concentration of CaCl2, the inoculated bacterial liquid will form the floccules, and its diameter sizes enlarged with the increasing concentration of CaCl2 and blocked the pores between the uranium tailings, resulting in nonuniform distribution of bacterial liquid and unstable transmission of cement solution in the sand column. It indicated that the fixation and uniform distribution of the bacterial liquid in the sand column cannot simultaneously reach their optimum value, especially to different sand samples. So it is necessary to conduct the experimental analysis according to actual needs.Therefore, in order to study the effects of different concentrations of the fixative solution on the fixation and migration ofSporosarcina Pasteurii in uranium tailings, five different concentrations of CaCl2 solution (0.005 mol/L, 0.015 mol/L, 0.025 mol/L, 0.035 mol/L, and 0.045 mol/L) were set to in the experiment. Based on the experimental results, the cement solution was determined with 0.5 mol/L urea and 0.5 mol/L CaCl2 solution (equivalent volume mixture).


## 2.4. Physical Model

The physical model of the sand columns were constructed of polyvinyl chloride (PVC) 7 cm height with an inner diameter of 6.18 cm (the same as the diameter size of the sand samples in the direct shear test). The upper part of the experimental device have a grouting port connected to the sealed plastic bottle with a rubber hose and a reserved vent. The grouting pipe and the outlet pipe have a rubber pipe with a water stop valve. In order to prevent the sand from entering the grouting pipe and outlet pipe, two gauze layers were set on both sides of the sand columns. And, two gravel layers (particle size 2 ± 0.5 mm) were laid on both sides of the sand column to avoid scouring the uranium tailings and clogging of the grouting mouth during the experiment. The experimental device is shown in Figure1 and Figure 2.Figure 1
Schematic diagram of the MICP experimental device.Figure 2
Physical model of the MICP experimental device.


## 3. Test Procedure and Methods

### 3.1. Activation and Propagation ofSporosarcina Pasteurii Strains

An inoculating loop was used to scrape the strains into a tube which contains 10 mL CASO liquid medium, and the tube was kept in a constant-temperature shaking incubator which was set at 30°C for 2 days. Then, an inoculating loop was used to take the culture supernatant and perform streak inoculation on a solid medium plate. Place the inoculated plate in a constant-temperature incubator which was set at 30°C for 2 days. After 2 days, the milky colonies formed on the solid medium (Figure3). An inoculating loop was used to scrape the strains from the solid medium into a conical flask with 100 mL of CASO liquid medium. Place the conical flask in a constant-temperature shaking incubator which was set at 30°C for 2 days. The rotation speed was controlled at 130 r/min. From Figure 4, obvious turbidness was observed in the inoculated conical flask compared with the blank reference after 48 h.Figure 3
Sporosarcina Pasteurii strains on solid medium.Figure 4
Sporosarcina Pasteurii strains were cultured in liquid medium for 2 days (left side is inoculated conical flask and the right side is blank reference).


### 3.2. Biochemical Characteristics of the Strains under Cement Solution

#### 3.2.1. Experiment Method

200 mL of cement solution was poured into three 250 ml of conical flasks. The OD600 value of bacterial liquid was diluted to 1.0 with CASO liquid medium. Then, 2 mL of the diluted bacterial liquid inoculated in the conical flask (inoculation amount was 1% (v/v)) which was placed in a constant-temperature shaking incubator which was set at 30°C. The rotation speed was controlled at 130 r/min. Then determine the number of bacteria in culture fluid, ammonia concentration, pH value, and CaCO3 production after being cultured for 2 h, 4 h, 8 h, 12 h, 18 h, 24 h, and 36 h.


#### 3.2.2. Detection Method

(1) Number of Bacteria. In this experiment, the protein nucleic acid analyzer was used to determine the OD600 value of the bacterial liquid and substitute the value into formula (1) [21] to calculate the total number of bacteria in the bacterial liquid.(1)Y=8.59×104Z1.3627,Where Z is the value of OD600 and Y is thethe concentration of bacterial liquid (units/μL).However, this formula is valid for the OD600 value between 0.2 and 0.8. Bacterial liquid should be diluted and then converted if it exceeds this range.(2) Ammonia Concentration. Take 40 mL of culture supernatant for different culture periods with a centrifuge tube and place them in a refrigerated centrifuge at a speed of 8000 r/min for 20 minutes. Pipette 10 mL of centrifuge supernatant into the colorimetric tube, and the ammonia concentration was determined by the spectrophotometric method.(3) pH Value. The pH value of the culture solution was measured by precision bench-top pH meter.(4) CaCO3 Production. The acid dissolution method was adopted to determine the calcium carbonate content in the mixed medium as follows: the culture liquid in the conical flask was filtered with filter paper after incubating in a constant-temperature shaking incubator for a period of time, and then put the filtered paper containing residues and the conical flask into a beaker at 70°C for 24 hours. The residues in the conical flask and the filter paper after drying are shown in Figure 5. The total mass of filter paper, beaker, and conical flask is W1.Figure 5
The residues in the conical flask and on the filter paper after drying.100 mL dilute hydrochloric acid (2 mol/L) was slowly added to the conical flask and to the beaker containing the filter paper, and stirred with a glass rod until no gas generated. After drying, the process was repeated once more, and the total mass of filter paper, beaker, and conical flask isW2. According to chemical reaction formula (2), the difference in mass between W2 and W1 before and after the reaction is the difference between CaCO3 and CaCl2. The content of CaCO3 can be calculated by the following formula:(2)mCaCO3=100×W2−W1111−100,(3)CaCO3100+2HCl⟶CaCl2111+H2O+CO2↑.


### 3.3. Domestication ofSporosarcina Pasteurii in the Radioactive Effluent of Uranium Tailings

2 ml bacterial suspension was inoculated into the conical flask which contains 10 mL CASO liquid medium and 100 ml sterilized radioactive effluent of uranium tailings. The flask was placed in the constant-temperature shaking incubator for 24 h which was set at 30°C, and the rotation speed was controlled at 200 r/min. The domesticated culture medium was taken by an inoculating loop and inoculated on a solid medium plate. Place the inoculated plate in a constant-temperature incubator which was set at 30°C for 2 days. After the strains grew up, they were added to the mixed medium again. The strains were domesticated once every 5 days for 8 successive generations.


### 3.4. Fine-Grained Uranium Tailings Bacterial Liquid Fixing Test

Firstly, the deionized water was slowly injected into the sand column to discharge the gas and to saturate the sand samples. The flow rate of the deionized water was controlled to 2 mL/min by adjusting the stop valve at the injection port. The OD600 value of the outflow liquid was detected and reached zero after 12 h. The water content of the saturated sand column is 38.9 mL by calculating the pore volume. According to related references, the inoculated amount of bacterial liquid is 1.2 times of water content of the saturated sand (50 mL). Bacterial liquid and CaCl2 solution were mixed at a ratio of 1 : 1, and the flow rate of mixed liquid was adjusted to 1 mL/min. After 12 h, the outlet water stop valve was opened, and 5 mL of effluent was collected to determine the OD600 value.


### 3.5. Fine-Grained Uranium Tailings Reinforcement Test

25 mL bacterial liquid (OD600 = 1.1) and CaCl2 solution were injected into the sand column with equal volume each day, and the injection flow rate was controlled at 1 mL/min. Eight sand column models inoculated with bacterial liquid were prepared for experiment, and another eight sand column models as a blank reference. Two molds were detached on the 4th, 8th, 12th, and 16th days, respectively; the uranium tailings reinforced by MICP is shown in Figure 6.Figure 6
Uranium tailings reinforced by MICP.After the sand column was vertically and smoothly placed into the geotechnical ring cutter, 0.5 cm × 0.5 cm × 0.5 cm sand samples were takeout with a soil spatula, and it was placed in a thermostatic oven dried for 24 hours at 105°C. The microstructure of the reinforced sand samples was observed by scanning electron microscopy (SEM) to observe the precipitation structure of CaCO3 and to analyze reinforcement mechanism of MICP.The geotechnical tests were conducted to determine the reinforcement effects of fine-grained uranium tailings by MICP, and the reinforced sand samples were loaded into the shear box to test the shear strength. The vertical normal stress is 50 kPa and 100 kPa. The control shear rate is 0.8 mm/min. The direct shear test is shown in Figure7.Figure 7
Direct shear test and shear failure of specimen.The sand samples after the test were ground with a grinder and dried in a thermostatic oven for 24 hours. 50 g of sand samples after grinding and 100 mL hydrochloric acid (2 mol/L) was added slowly into a beaker and stirred with a glass rod until no gas generated. Then, place it in a thermostatic oven for 24 hours, and the process was repeated once more. After drying, the sand samples were weighed inm′.The mass difference betweenm′ and 50 before and after the reaction were the mass difference between CaCO3 and CaCl2. According to the chemical equation, the mass of CaCO3 can be calculated by the following:.(4)mCaCo3=100×m′−50111−100.The calculated mass of CaCO3 subtracted with the original mass of CaCO3 was the mass of CaCO3 generated in each sand column. The volume of sand samples was calculated according to the density of each sand column, so the mass of CaCO3 produced per unit volume can be calculated.


## 3.1. Activation and Propagation ofSporosarcina Pasteurii Strains

An inoculating loop was used to scrape the strains into a tube which contains 10 mL CASO liquid medium, and the tube was kept in a constant-temperature shaking incubator which was set at 30°C for 2 days. Then, an inoculating loop was used to take the culture supernatant and perform streak inoculation on a solid medium plate. Place the inoculated plate in a constant-temperature incubator which was set at 30°C for 2 days. After 2 days, the milky colonies formed on the solid medium (Figure3). An inoculating loop was used to scrape the strains from the solid medium into a conical flask with 100 mL of CASO liquid medium. Place the conical flask in a constant-temperature shaking incubator which was set at 30°C for 2 days. The rotation speed was controlled at 130 r/min. From Figure 4, obvious turbidness was observed in the inoculated conical flask compared with the blank reference after 48 h.Figure 3
Sporosarcina Pasteurii strains on solid medium.Figure 4
Sporosarcina Pasteurii strains were cultured in liquid medium for 2 days (left side is inoculated conical flask and the right side is blank reference).


## 3.2. Biochemical Characteristics of the Strains under Cement Solution

### 3.2.1. Experiment Method

200 mL of cement solution was poured into three 250 ml of conical flasks. The OD600 value of bacterial liquid was diluted to 1.0 with CASO liquid medium. Then, 2 mL of the diluted bacterial liquid inoculated in the conical flask (inoculation amount was 1% (v/v)) which was placed in a constant-temperature shaking incubator which was set at 30°C. The rotation speed was controlled at 130 r/min. Then determine the number of bacteria in culture fluid, ammonia concentration, pH value, and CaCO3 production after being cultured for 2 h, 4 h, 8 h, 12 h, 18 h, 24 h, and 36 h.


### 3.2.2. Detection Method

(1) Number of Bacteria. In this experiment, the protein nucleic acid analyzer was used to determine the OD600 value of the bacterial liquid and substitute the value into formula (1) [21] to calculate the total number of bacteria in the bacterial liquid.(1)Y=8.59×104Z1.3627,Where Z is the value of OD600 and Y is thethe concentration of bacterial liquid (units/μL).However, this formula is valid for the OD600 value between 0.2 and 0.8. Bacterial liquid should be diluted and then converted if it exceeds this range.(2) Ammonia Concentration. Take 40 mL of culture supernatant for different culture periods with a centrifuge tube and place them in a refrigerated centrifuge at a speed of 8000 r/min for 20 minutes. Pipette 10 mL of centrifuge supernatant into the colorimetric tube, and the ammonia concentration was determined by the spectrophotometric method.(3) pH Value. The pH value of the culture solution was measured by precision bench-top pH meter.(4) CaCO3 Production. The acid dissolution method was adopted to determine the calcium carbonate content in the mixed medium as follows: the culture liquid in the conical flask was filtered with filter paper after incubating in a constant-temperature shaking incubator for a period of time, and then put the filtered paper containing residues and the conical flask into a beaker at 70°C for 24 hours. The residues in the conical flask and the filter paper after drying are shown in Figure 5. The total mass of filter paper, beaker, and conical flask is W1.Figure 5
The residues in the conical flask and on the filter paper after drying.100 mL dilute hydrochloric acid (2 mol/L) was slowly added to the conical flask and to the beaker containing the filter paper, and stirred with a glass rod until no gas generated. After drying, the process was repeated once more, and the total mass of filter paper, beaker, and conical flask isW2. According to chemical reaction formula (2), the difference in mass between W2 and W1 before and after the reaction is the difference between CaCO3 and CaCl2. The content of CaCO3 can be calculated by the following formula:(2)mCaCO3=100×W2−W1111−100,(3)CaCO3100+2HCl⟶CaCl2111+H2O+CO2↑.


## 3.2.1. Experiment Method

200 mL of cement solution was poured into three 250 ml of conical flasks. The OD600 value of bacterial liquid was diluted to 1.0 with CASO liquid medium. Then, 2 mL of the diluted bacterial liquid inoculated in the conical flask (inoculation amount was 1% (v/v)) which was placed in a constant-temperature shaking incubator which was set at 30°C. The rotation speed was controlled at 130 r/min. Then determine the number of bacteria in culture fluid, ammonia concentration, pH value, and CaCO3 production after being cultured for 2 h, 4 h, 8 h, 12 h, 18 h, 24 h, and 36 h.


## 3.2.2. Detection Method

(1) Number of Bacteria. In this experiment, the protein nucleic acid analyzer was used to determine the OD600 value of the bacterial liquid and substitute the value into formula (1) [21] to calculate the total number of bacteria in the bacterial liquid.(1)Y=8.59×104Z1.3627,Where Z is the value of OD600 and Y is thethe concentration of bacterial liquid (units/μL).However, this formula is valid for the OD600 value between 0.2 and 0.8. Bacterial liquid should be diluted and then converted if it exceeds this range.(2) Ammonia Concentration. Take 40 mL of culture supernatant for different culture periods with a centrifuge tube and place them in a refrigerated centrifuge at a speed of 8000 r/min for 20 minutes. Pipette 10 mL of centrifuge supernatant into the colorimetric tube, and the ammonia concentration was determined by the spectrophotometric method.(3) pH Value. The pH value of the culture solution was measured by precision bench-top pH meter.(4) CaCO3 Production. The acid dissolution method was adopted to determine the calcium carbonate content in the mixed medium as follows: the culture liquid in the conical flask was filtered with filter paper after incubating in a constant-temperature shaking incubator for a period of time, and then put the filtered paper containing residues and the conical flask into a beaker at 70°C for 24 hours. The residues in the conical flask and the filter paper after drying are shown in Figure 5. The total mass of filter paper, beaker, and conical flask is W1.Figure 5
The residues in the conical flask and on the filter paper after drying.100 mL dilute hydrochloric acid (2 mol/L) was slowly added to the conical flask and to the beaker containing the filter paper, and stirred with a glass rod until no gas generated. After drying, the process was repeated once more, and the total mass of filter paper, beaker, and conical flask isW2. According to chemical reaction formula (2), the difference in mass between W2 and W1 before and after the reaction is the difference between CaCO3 and CaCl2. The content of CaCO3 can be calculated by the following formula:(2)mCaCO3=100×W2−W1111−100,(3)CaCO3100+2HCl⟶CaCl2111+H2O+CO2↑.


## 3.3. Domestication ofSporosarcina Pasteurii in the Radioactive Effluent of Uranium Tailings

2 ml bacterial suspension was inoculated into the conical flask which contains 10 mL CASO liquid medium and 100 ml sterilized radioactive effluent of uranium tailings. The flask was placed in the constant-temperature shaking incubator for 24 h which was set at 30°C, and the rotation speed was controlled at 200 r/min. The domesticated culture medium was taken by an inoculating loop and inoculated on a solid medium plate. Place the inoculated plate in a constant-temperature incubator which was set at 30°C for 2 days. After the strains grew up, they were added to the mixed medium again. The strains were domesticated once every 5 days for 8 successive generations.


## 3.4. Fine-Grained Uranium Tailings Bacterial Liquid Fixing Test

Firstly, the deionized water was slowly injected into the sand column to discharge the gas and to saturate the sand samples. The flow rate of the deionized water was controlled to 2 mL/min by adjusting the stop valve at the injection port. The OD600 value of the outflow liquid was detected and reached zero after 12 h. The water content of the saturated sand column is 38.9 mL by calculating the pore volume. According to related references, the inoculated amount of bacterial liquid is 1.2 times of water content of the saturated sand (50 mL). Bacterial liquid and CaCl2 solution were mixed at a ratio of 1 : 1, and the flow rate of mixed liquid was adjusted to 1 mL/min. After 12 h, the outlet water stop valve was opened, and 5 mL of effluent was collected to determine the OD600 value.


## 3.5. Fine-Grained Uranium Tailings Reinforcement Test

25 mL bacterial liquid (OD600 = 1.1) and CaCl2 solution were injected into the sand column with equal volume each day, and the injection flow rate was controlled at 1 mL/min. Eight sand column models inoculated with bacterial liquid were prepared for experiment, and another eight sand column models as a blank reference. Two molds were detached on the 4th, 8th, 12th, and 16th days, respectively; the uranium tailings reinforced by MICP is shown in Figure 6.Figure 6
Uranium tailings reinforced by MICP.After the sand column was vertically and smoothly placed into the geotechnical ring cutter, 0.5 cm × 0.5 cm × 0.5 cm sand samples were takeout with a soil spatula, and it was placed in a thermostatic oven dried for 24 hours at 105°C. The microstructure of the reinforced sand samples was observed by scanning electron microscopy (SEM) to observe the precipitation structure of CaCO3 and to analyze reinforcement mechanism of MICP.The geotechnical tests were conducted to determine the reinforcement effects of fine-grained uranium tailings by MICP, and the reinforced sand samples were loaded into the shear box to test the shear strength. The vertical normal stress is 50 kPa and 100 kPa. The control shear rate is 0.8 mm/min. The direct shear test is shown in Figure7.Figure 7
Direct shear test and shear failure of specimen.The sand samples after the test were ground with a grinder and dried in a thermostatic oven for 24 hours. 50 g of sand samples after grinding and 100 mL hydrochloric acid (2 mol/L) was added slowly into a beaker and stirred with a glass rod until no gas generated. Then, place it in a thermostatic oven for 24 hours, and the process was repeated once more. After drying, the sand samples were weighed inm′.The mass difference betweenm′ and 50 before and after the reaction were the mass difference between CaCO3 and CaCl2. According to the chemical equation, the mass of CaCO3 can be calculated by the following:.(4)mCaCo3=100×m′−50111−100.The calculated mass of CaCO3 subtracted with the original mass of CaCO3 was the mass of CaCO3 generated in each sand column. The volume of sand samples was calculated according to the density of each sand column, so the mass of CaCO3 produced per unit volume can be calculated.


## 4. Test results

### 4.1. Physiological and Biochemical Characteristics of Strains in the Environment of Cement

#### 4.1.1. The Changes in Bacterial Number and pH Value as Function of Time

The changes in bacterial number and pH value in mixed media as function of time was obtained by culturingSporosarcina Pasteurii for 36 h in a shake flask, as shown in Figure 8. The growth curve of bacterial number basically conforms to the Gompertz–Richards model and could be divided into three stages. The lag stage phase (0–2 h) is not very obvious for the Sporosarcina Pasteurii chosen for the experiment is excellent and has adapted to the test by cycle culturing. In this stage, the pH value of the solution did not change substantially. Almost no increase in the number of Sporosarcina Pasteurii was observed, but its anabolism was active to provide sufficient enzymes, energy, and intermediate metabolites for subsequent growth and reproduction of bacteria.Figure 8
Changes in bacterial density and pH value in mixed media as function of time.In the logarithmic phase (2–18 h), the number of bacteria has a steady geometric growth. The biological morphology was typical, and the biological activity was strong. A large number of urease was produced to decompose the urea into carbonate and ammonium ions, so the pH value of solution rises from 7.3 to about 9.0. Therefore, it is suitable for choosing the strains in this phase for the following inoculation experiment.In the stationary phase (18–24 h), the number of bacteria reached a peak and tended to be stable, but high activity of the strains was maintained. Some adverse effects have been appeared such as the overconsumption of nutrients in the medium, the accumulation of toxic metabolites (such as alcohol and H2O2), the increase of pH causing changes in bacterial morphology and biological activity, decreased bacterial growth rate, and increased relative death of bacteria, but bacterial number attains equilibrium between proliferation and death, and the spores began to form in this phase.In the decline phase (after 24 h), the bacterial number tended to increase slowly and the number of dead bacteria increased significantly. The number of live bacteria was inversely related with the culture time, and the pH value of the solution was slightly declined, and the physiological metabolic activity tends to stagnant.


#### 4.1.2. The Changes in Ammonia Nitrogen Concentration and CaCO3 Production as Function of Time

The changes in ammonia nitrogen concentration and CaCO3 production as function of time was obtained by shaking flask experiments in cement solution, as shown in Figure 9. After inoculating the Sporosarcina Pasteurii into the mixed culture medium for 4 hours, the concentration of ammonia nitrogen rose rapidly, which indicated that Sporosarcina Pasteurii had a high urease activity in the initial state, but the production rate of CaCO3 increase slowly because of the bacteria are still in the lag phase or logarithmic phase which have insufficient bacteria and urease. In the following 14 hours, the bacteria maintained a high level of physiological activity which resulted in a continuous increase of CaCO3 production. And, the ammonia nitrogen production began to decline because ammonia nitrogen volatilize to the outside during the shaking culture.Figure 9
The changes in ammonia nitrogen concentration and CaCO3 production as function of time.At 18 h after inoculation, production rate of the calcium carbonate slows down. On the one hand, the ratio of calcium ions converted into crystals in the solution reached 80%, and calcium ion concentration has become the limiting factor to affect the process of microbial mineralization. On the other hand, the activity of the bacteria decreased because of limited nutrients and deteriorated external conditions in the culture medium. Especially, the amount of calcium carbonate remains stable as the bacteria entered the decline stage after 24 h. The results showed the following: CaCO3 production of Sporosarcina Pasteurii in the cement solution was positively correlated with its physiological activity and calcium concentration.


### 4.2. Test Results of Bacterial Liquid Fixation in Uranium Tailings Sand

Different concentrations of CaCl2 solution and bacterial liquid were injected into the sand column 12 h later, the effluent was collected, its concentrations of CaCl2 and the OD600 value were determined, and the results are shown in Figure 10. We can see that the higher the concentration of CaCl2 solution is, the lower the OD600 value of the effluent is, and the value was followed by a logarithmic decrease. However, considering the low concentration of CaCl2 solution is conducive to the transmission of bacteria liquid, 0.025 mol/L CaCl2 solution was chosen as the fixative solution in the experiment, and the fixation rate of bacterial liquid was 92.14% by experiment (the fixation rate was higher than 90%).Figure 10
Curve of the OD600 value of effluent with the concentration of CaCl2 solution.


### 4.3. Test of Uranium Tailings Reinforced by MICP

#### 4.3.1. Changes of Shear Strength of Reinforced Uranium Tailings

The direct shear was used to test the variation of shear properties of reinforced uranium tailings over time. The results are shown in Figure11; we can see the internal friction angle, cohesive force, and shear strength of uranium tailings reinforced by MICP were significantly increased. And, the shear strength growth rate of grouting reinforcement from 8 to 12 days is the highest due to the calcium carbonate crystals binds sand particles together and reinforces the structure strength of the sand. After 16 days, the cohesion of reinforced uranium tailings increased from 9.59 kPa to 23.03 kPa, the growth rate was 140.1%, the internal friction angle of reinforced uranium tailings increased from 29.1° to 42.7°, and the growth rate was 46.7%. Under the normal stress of 50 kPa, the shear strength increased from 37.42 kPa to 69.02 kPa, and the growth rate was 84.6%. Under the normal stress of 100 kPa, the shear strength increased from 65.25 kPa to 115.14 kPa, and the growth rate was 76.5%. However, the shear strength of the blank reference group did not change substantially. The results showed that MICP technology can increase shear strength of uranium tailings effectively.Figure 11
The changes in shear strength of uranium tailings reinforced by MICP as function of time.


#### 4.3.2. The Change in CaCO3 Production in Uranium Tailings as Function of Time

The change of CaCO3 production was determined by the acid dissolution method, the changes in CaCO3 production as a function of time are shown in Figure 12, and utilization rate of calcium ion of MICP was calculated after experiment; the results are shown in Table 2, about 65%. We can see that the inoculated group has more CaCO3 production and higher calcium ion utilization rate than blank reference group. However, the utilization rate should be improved compared with the 90% utilization rate of shaking flask experiment in the cement liquid in 4.1.Figure 12
The changes in CaCO3 production of uranium tailings reinforced by MICP as function of time.Table 2
Table of calcium ion utilization.
Curing time
4 d
8 d
12 d
16 d
Calcium ion utilization rate of MICP
65.56%
67.34%
64.31%
62.46%
Calcium ion utilization rate of blank reference
9.55%
2.93%
2.78%
1.78%To further research the differences in CaCO3 production of MICP between the uranium tailings environment and the cement liquid environment, detaching the molds and observing the cementation of different layers of sand column after grouting (Figure 13), a lot of CaCO3 crystal deposits were found in the buffer gravel layer in the grouting hole and in the gauze layer. Therefore, the reason for the low utilization rate of calcium ion in uranium tailings is that CaCO3 particles were deposited in the gravel layer and gauze layer, or the bacteria were washed away during the grouting process, resulting in insufficient nucleus to form the CaCO3.Figure 13
Deposition of CaCO3 in gravel layers and gauze layers: (a) buffer gravel layer; (b) grouting mouth and gauze layer.
(a)
(b)


#### 4.3.3. SEM Results and Analysis of Reinforcement Mechanism

To research the reinforcement mechanism of uranium tailings reinforced by MICP, SEM images were observed for sand samples after the experiment. The scanning electron micrographs are presented in Figure14; it can be observed that, between the uranium tailings, particles were filled with a large number of white calcite crystals which are irregular particles, and there are overlapping phenomena. These crystals bind uranium tailings together and form bio-sandstones, which reduces the porosity of sand samples, and greatly improve their shear strength.Figure 14
SEM images of uranium tailings reinforced by MICP at the end of experiment (200x : a; 400x : b).
(a)
(b)


## 4.1. Physiological and Biochemical Characteristics of Strains in the Environment of Cement

### 4.1.1. The Changes in Bacterial Number and pH Value as Function of Time

The changes in bacterial number and pH value in mixed media as function of time was obtained by culturingSporosarcina Pasteurii for 36 h in a shake flask, as shown in Figure 8. The growth curve of bacterial number basically conforms to the Gompertz–Richards model and could be divided into three stages. The lag stage phase (0–2 h) is not very obvious for the Sporosarcina Pasteurii chosen for the experiment is excellent and has adapted to the test by cycle culturing. In this stage, the pH value of the solution did not change substantially. Almost no increase in the number of Sporosarcina Pasteurii was observed, but its anabolism was active to provide sufficient enzymes, energy, and intermediate metabolites for subsequent growth and reproduction of bacteria.Figure 8
Changes in bacterial density and pH value in mixed media as function of time.In the logarithmic phase (2–18 h), the number of bacteria has a steady geometric growth. The biological morphology was typical, and the biological activity was strong. A large number of urease was produced to decompose the urea into carbonate and ammonium ions, so the pH value of solution rises from 7.3 to about 9.0. Therefore, it is suitable for choosing the strains in this phase for the following inoculation experiment.In the stationary phase (18–24 h), the number of bacteria reached a peak and tended to be stable, but high activity of the strains was maintained. Some adverse effects have been appeared such as the overconsumption of nutrients in the medium, the accumulation of toxic metabolites (such as alcohol and H2O2), the increase of pH causing changes in bacterial morphology and biological activity, decreased bacterial growth rate, and increased relative death of bacteria, but bacterial number attains equilibrium between proliferation and death, and the spores began to form in this phase.In the decline phase (after 24 h), the bacterial number tended to increase slowly and the number of dead bacteria increased significantly. The number of live bacteria was inversely related with the culture time, and the pH value of the solution was slightly declined, and the physiological metabolic activity tends to stagnant.


### 4.1.2. The Changes in Ammonia Nitrogen Concentration and CaCO3 Production as Function of Time

The changes in ammonia nitrogen concentration and CaCO3 production as function of time was obtained by shaking flask experiments in cement solution, as shown in Figure 9. After inoculating the Sporosarcina Pasteurii into the mixed culture medium for 4 hours, the concentration of ammonia nitrogen rose rapidly, which indicated that Sporosarcina Pasteurii had a high urease activity in the initial state, but the production rate of CaCO3 increase slowly because of the bacteria are still in the lag phase or logarithmic phase which have insufficient bacteria and urease. In the following 14 hours, the bacteria maintained a high level of physiological activity which resulted in a continuous increase of CaCO3 production. And, the ammonia nitrogen production began to decline because ammonia nitrogen volatilize to the outside during the shaking culture.Figure 9
The changes in ammonia nitrogen concentration and CaCO3 production as function of time.At 18 h after inoculation, production rate of the calcium carbonate slows down. On the one hand, the ratio of calcium ions converted into crystals in the solution reached 80%, and calcium ion concentration has become the limiting factor to affect the process of microbial mineralization. On the other hand, the activity of the bacteria decreased because of limited nutrients and deteriorated external conditions in the culture medium. Especially, the amount of calcium carbonate remains stable as the bacteria entered the decline stage after 24 h. The results showed the following: CaCO3 production of Sporosarcina Pasteurii in the cement solution was positively correlated with its physiological activity and calcium concentration.


## 4.1.1. The Changes in Bacterial Number and pH Value as Function of Time

The changes in bacterial number and pH value in mixed media as function of time was obtained by culturingSporosarcina Pasteurii for 36 h in a shake flask, as shown in Figure 8. The growth curve of bacterial number basically conforms to the Gompertz–Richards model and could be divided into three stages. The lag stage phase (0–2 h) is not very obvious for the Sporosarcina Pasteurii chosen for the experiment is excellent and has adapted to the test by cycle culturing. In this stage, the pH value of the solution did not change substantially. Almost no increase in the number of Sporosarcina Pasteurii was observed, but its anabolism was active to provide sufficient enzymes, energy, and intermediate metabolites for subsequent growth and reproduction of bacteria.Figure 8
Changes in bacterial density and pH value in mixed media as function of time.In the logarithmic phase (2–18 h), the number of bacteria has a steady geometric growth. The biological morphology was typical, and the biological activity was strong. A large number of urease was produced to decompose the urea into carbonate and ammonium ions, so the pH value of solution rises from 7.3 to about 9.0. Therefore, it is suitable for choosing the strains in this phase for the following inoculation experiment.In the stationary phase (18–24 h), the number of bacteria reached a peak and tended to be stable, but high activity of the strains was maintained. Some adverse effects have been appeared such as the overconsumption of nutrients in the medium, the accumulation of toxic metabolites (such as alcohol and H2O2), the increase of pH causing changes in bacterial morphology and biological activity, decreased bacterial growth rate, and increased relative death of bacteria, but bacterial number attains equilibrium between proliferation and death, and the spores began to form in this phase.In the decline phase (after 24 h), the bacterial number tended to increase slowly and the number of dead bacteria increased significantly. The number of live bacteria was inversely related with the culture time, and the pH value of the solution was slightly declined, and the physiological metabolic activity tends to stagnant.


## 4.1.2. The Changes in Ammonia Nitrogen Concentration and CaCO3 Production as Function of Time

The changes in ammonia nitrogen concentration and CaCO3 production as function of time was obtained by shaking flask experiments in cement solution, as shown in Figure 9. After inoculating the Sporosarcina Pasteurii into the mixed culture medium for 4 hours, the concentration of ammonia nitrogen rose rapidly, which indicated that Sporosarcina Pasteurii had a high urease activity in the initial state, but the production rate of CaCO3 increase slowly because of the bacteria are still in the lag phase or logarithmic phase which have insufficient bacteria and urease. In the following 14 hours, the bacteria maintained a high level of physiological activity which resulted in a continuous increase of CaCO3 production. And, the ammonia nitrogen production began to decline because ammonia nitrogen volatilize to the outside during the shaking culture.Figure 9
The changes in ammonia nitrogen concentration and CaCO3 production as function of time.At 18 h after inoculation, production rate of the calcium carbonate slows down. On the one hand, the ratio of calcium ions converted into crystals in the solution reached 80%, and calcium ion concentration has become the limiting factor to affect the process of microbial mineralization. On the other hand, the activity of the bacteria decreased because of limited nutrients and deteriorated external conditions in the culture medium. Especially, the amount of calcium carbonate remains stable as the bacteria entered the decline stage after 24 h. The results showed the following: CaCO3 production of Sporosarcina Pasteurii in the cement solution was positively correlated with its physiological activity and calcium concentration.


## 4.2. Test Results of Bacterial Liquid Fixation in Uranium Tailings Sand

Different concentrations of CaCl2 solution and bacterial liquid were injected into the sand column 12 h later, the effluent was collected, its concentrations of CaCl2 and the OD600 value were determined, and the results are shown in Figure 10. We can see that the higher the concentration of CaCl2 solution is, the lower the OD600 value of the effluent is, and the value was followed by a logarithmic decrease. However, considering the low concentration of CaCl2 solution is conducive to the transmission of bacteria liquid, 0.025 mol/L CaCl2 solution was chosen as the fixative solution in the experiment, and the fixation rate of bacterial liquid was 92.14% by experiment (the fixation rate was higher than 90%).Figure 10
Curve of the OD600 value of effluent with the concentration of CaCl2 solution.


## 4.3. Test of Uranium Tailings Reinforced by MICP

### 4.3.1. Changes of Shear Strength of Reinforced Uranium Tailings

The direct shear was used to test the variation of shear properties of reinforced uranium tailings over time. The results are shown in Figure11; we can see the internal friction angle, cohesive force, and shear strength of uranium tailings reinforced by MICP were significantly increased. And, the shear strength growth rate of grouting reinforcement from 8 to 12 days is the highest due to the calcium carbonate crystals binds sand particles together and reinforces the structure strength of the sand. After 16 days, the cohesion of reinforced uranium tailings increased from 9.59 kPa to 23.03 kPa, the growth rate was 140.1%, the internal friction angle of reinforced uranium tailings increased from 29.1° to 42.7°, and the growth rate was 46.7%. Under the normal stress of 50 kPa, the shear strength increased from 37.42 kPa to 69.02 kPa, and the growth rate was 84.6%. Under the normal stress of 100 kPa, the shear strength increased from 65.25 kPa to 115.14 kPa, and the growth rate was 76.5%. However, the shear strength of the blank reference group did not change substantially. The results showed that MICP technology can increase shear strength of uranium tailings effectively.Figure 11
The changes in shear strength of uranium tailings reinforced by MICP as function of time.


### 4.3.2. The Change in CaCO3 Production in Uranium Tailings as Function of Time

The change of CaCO3 production was determined by the acid dissolution method, the changes in CaCO3 production as a function of time are shown in Figure 12, and utilization rate of calcium ion of MICP was calculated after experiment; the results are shown in Table 2, about 65%. We can see that the inoculated group has more CaCO3 production and higher calcium ion utilization rate than blank reference group. However, the utilization rate should be improved compared with the 90% utilization rate of shaking flask experiment in the cement liquid in 4.1.Figure 12
The changes in CaCO3 production of uranium tailings reinforced by MICP as function of time.Table 2
Table of calcium ion utilization.
Curing time
4 d
8 d
12 d
16 d
Calcium ion utilization rate of MICP
65.56%
67.34%
64.31%
62.46%
Calcium ion utilization rate of blank reference
9.55%
2.93%
2.78%
1.78%To further research the differences in CaCO3 production of MICP between the uranium tailings environment and the cement liquid environment, detaching the molds and observing the cementation of different layers of sand column after grouting (Figure 13), a lot of CaCO3 crystal deposits were found in the buffer gravel layer in the grouting hole and in the gauze layer. Therefore, the reason for the low utilization rate of calcium ion in uranium tailings is that CaCO3 particles were deposited in the gravel layer and gauze layer, or the bacteria were washed away during the grouting process, resulting in insufficient nucleus to form the CaCO3.Figure 13
Deposition of CaCO3 in gravel layers and gauze layers: (a) buffer gravel layer; (b) grouting mouth and gauze layer.
(a)
(b)


### 4.3.3. SEM Results and Analysis of Reinforcement Mechanism

To research the reinforcement mechanism of uranium tailings reinforced by MICP, SEM images were observed for sand samples after the experiment. The scanning electron micrographs are presented in Figure14; it can be observed that, between the uranium tailings, particles were filled with a large number of white calcite crystals which are irregular particles, and there are overlapping phenomena. These crystals bind uranium tailings together and form bio-sandstones, which reduces the porosity of sand samples, and greatly improve their shear strength.Figure 14
SEM images of uranium tailings reinforced by MICP at the end of experiment (200x : a; 400x : b).
(a)
(b)


## 4.3.1. Changes of Shear Strength of Reinforced Uranium Tailings

The direct shear was used to test the variation of shear properties of reinforced uranium tailings over time. The results are shown in Figure11; we can see the internal friction angle, cohesive force, and shear strength of uranium tailings reinforced by MICP were significantly increased. And, the shear strength growth rate of grouting reinforcement from 8 to 12 days is the highest due to the calcium carbonate crystals binds sand particles together and reinforces the structure strength of the sand. After 16 days, the cohesion of reinforced uranium tailings increased from 9.59 kPa to 23.03 kPa, the growth rate was 140.1%, the internal friction angle of reinforced uranium tailings increased from 29.1° to 42.7°, and the growth rate was 46.7%. Under the normal stress of 50 kPa, the shear strength increased from 37.42 kPa to 69.02 kPa, and the growth rate was 84.6%. Under the normal stress of 100 kPa, the shear strength increased from 65.25 kPa to 115.14 kPa, and the growth rate was 76.5%. However, the shear strength of the blank reference group did not change substantially. The results showed that MICP technology can increase shear strength of uranium tailings effectively.Figure 11
The changes in shear strength of uranium tailings reinforced by MICP as function of time.


## 4.3.2. The Change in CaCO3 Production in Uranium Tailings as Function of Time

The change of CaCO3 production was determined by the acid dissolution method, the changes in CaCO3 production as a function of time are shown in Figure 12, and utilization rate of calcium ion of MICP was calculated after experiment; the results are shown in Table 2, about 65%. We can see that the inoculated group has more CaCO3 production and higher calcium ion utilization rate than blank reference group. However, the utilization rate should be improved compared with the 90% utilization rate of shaking flask experiment in the cement liquid in 4.1.Figure 12
The changes in CaCO3 production of uranium tailings reinforced by MICP as function of time.Table 2
Table of calcium ion utilization.
Curing time
4 d
8 d
12 d
16 d
Calcium ion utilization rate of MICP
65.56%
67.34%
64.31%
62.46%
Calcium ion utilization rate of blank reference
9.55%
2.93%
2.78%
1.78%To further research the differences in CaCO3 production of MICP between the uranium tailings environment and the cement liquid environment, detaching the molds and observing the cementation of different layers of sand column after grouting (Figure 13), a lot of CaCO3 crystal deposits were found in the buffer gravel layer in the grouting hole and in the gauze layer. Therefore, the reason for the low utilization rate of calcium ion in uranium tailings is that CaCO3 particles were deposited in the gravel layer and gauze layer, or the bacteria were washed away during the grouting process, resulting in insufficient nucleus to form the CaCO3.Figure 13
Deposition of CaCO3 in gravel layers and gauze layers: (a) buffer gravel layer; (b) grouting mouth and gauze layer.
(a)
(b)


## 4.3.3. SEM Results and Analysis of Reinforcement Mechanism

To research the reinforcement mechanism of uranium tailings reinforced by MICP, SEM images were observed for sand samples after the experiment. The scanning electron micrographs are presented in Figure14; it can be observed that, between the uranium tailings, particles were filled with a large number of white calcite crystals which are irregular particles, and there are overlapping phenomena. These crystals bind uranium tailings together and form bio-sandstones, which reduces the porosity of sand samples, and greatly improve their shear strength.Figure 14
SEM images of uranium tailings reinforced by MICP at the end of experiment (200x : a; 400x : b).
(a)
(b)


## 5. Conclusion

In this paper,Sporosarcina Pasteurii was chosen for the experiment to study the effect and mechanism of fine-grained uranium tailings reinforced by MICP. The experiments were performed in shaking cement solution and in sand column, and biochemical properties and mineralization efficiency were analyzed. The effect and mechanism of uranium tailings reinforced by MICP were discussed by direct shear test and SEM. The results obtained are as follows:(1)
The growth curve of bacterial number basically conforms with the Gompertz–Richards model. The lag stage phase of curve is not very obvious which indicated that theSporosarcina Pasteurii chosen for the experiment is excellent. Through shaking flask experiments in cement solution, it indicated that the CaCO3 production of Sporosarcina Pasteurii in the cement solution was positively correlated with its physiological activity and the concentration of calcium sources, and it showed an increase at the first and then tended to be stable.(2)
The following can be found from the microbial immobilization experiments: the higher the solution concentration of CaCl2, the lower the discharge rate of bacterial liquid from the sand column, but high concentration of CaCl2 solution will affect the uniform distribution and migration of bacteria in the uranium tailings.(3)
Sporosarcina Pasteurii was chosen for MICP experiment for 16 d, and the shear strength of uranium tailings increased by 84.6% and 76.5%, respectively, under 50 kPa and 100 kPa normal press. The results show that the MICP technology can increase the shear strength of fine-grained uranium tailings effectively. Through the scanning electron micrographs, the mechanism of uranium tailings reinforced by MICP was that calcite crystals produced by Sporosarcina Pasteurii bind the uranium tailings particles together and form biological sandstones.(4)
The physical model and grouting mode should be further optimized to improve the production rate of CaCO3. And, the external factors (e.g., temperature and pressure) that influence the reinforced effect of uranium tailings by MICP should be considered, and the reinforcement depth should be further studied in the following experiments.



---

*Source: 2928985-2018-10-17.xml*