# Chemical Compounds of Malacca Leaf (Phyllanthus emblica) after Triple Extraction with N-Hexane, Ethyl Acetate, and Ethanol

Authors: Nuzul Asmilia; Yudha Fahrimal; Mahdi Abrar; Rinidar Rinidar
Journal: The Scientific World Journal
 (2020)

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## Abstract

Malacca (Phyllanthus emblica) is one of the plants that is often by the community in the Aceh Besar district of Indonesia as a traditional medicine for the treatment of various diseases such as antimicrobial, antibacterial, antifungals, antivirals, antimutagenic, antimalaria, and antiallergic. This research was conducted to analyze the content of chemical compounds in the ethanol extract of the Malacca leaf (EEDM) using a gas chromatography-mass spectrophotometer (GC-MS). Malacca leaves were extracted by the maceration method using n-hexane, ethyl acetate, and ethanol. The GC-MS analysis showed EEDM contained 22 chemical compounds. The highest chemical content of EEDM is octadecanoic acid reaching 22.93%, 9,12-octadecanoic acid 14.99%, octadecanoic acid 7.59%, 9-hexadecenoic acid 6.17%, octadecanoic acid 5.95%, octadecanal 5.59%, 9,12-octadecanoic acid 5.06%, 3-eicosyne 4.75%, 1-hexadecenoic acid 4.08%, 11-tetradecen-1-ol 2.92%, 2-furanmethanol 2.83%, delta-guaiene 2.43%, cyclohexane 2.13%, hexadecanoic acid 1.99%, sativen 1.87%, octadecanoic acid 1.52%, 1H-cyclopropaanaphthalene 1.40%, tetradecanoic acid 1.40%, 3,7,11-tridecatrienenitrile 1.20%, caryophellene 1.11%, 2H-pyran 1.07%, and trans-caryophellene 1.03%. This study clearly shows the presence of fatty acids which play a major role in the efficacy of these traditional medicines particularly as antioxidant and antimalarial.


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## Body

## 1. Introduction

Malacca (Phyllanthus emblica) is one of the plants that is often used by the community in the Aceh Besar district of Indonesia as a traditional medicine for the treatment of various diseases. Phyllanthus emblica in Indonesia is known as the kimalaka [1]. The plant is also called “bak rem“ (Acehnese), balakka in North Sumatra, metengo in Ternate [2], and kemloko in Java [3]. In English, these plants are referred as gooseberry Indians [4], and India is known by various names such as aonla, nelli, amla, amlika, dhotri, emblica, and usuri [5] In Malaysia, it is called diaper melaka, in German called amla [6], and in Thailand called ma-kham-pom [7].Malacca is widespread in most tropical countries and subtropical countries and is native to South and Southeast Asia [8]. This plant belongs to the family Euphorbiaceae which is widely distributed and grows wild in India, Sri Lanka, Pakistan, Uzbekistan, China, Indonesia, Malaysia, and Thailand. Malacca can grow on the hillside with a height of 200 meters and can be cultivated [9, 10]. The identification of Phyllanthus emblica Linn., has a very large genus of around 550 to 750 species which are divided into 10 to 11 subgenera [11].Numerous studies have been done on the Malacca plant which reported that the Malacca plant has a potency as chemoprotective and antioxidant [6], analgesic, antipyretic [12], antitumor [13, 14], anti-inflammation [15, 16], able to decrease blood sugar level [17], antiviral, antimutagenic, and antiallergic [18], antimicrobial, and antibacterial antifungals [19, 20]. Malar and Mary [21] proved that the fruit of Malacca can appear to be able to repair liver damage, and Asmilia et al. [22] reported that the ethanolic extract of Malacca leaves has a higher inhibition level on Plasmodium falciparum growth compared to ethyl acetate and n-hexane extract of Malacca leaves. Deepak and Gopal [23] found terpenes, phytosterols, and terpenoids in ethyl acetate extracts from the bark of Phyllanthus emblica. So far, there has been no report on the content of the chemical compounds of Malacca leaves triple extracted using n-hexane, ethyl acetate, and ethanol solution. Therefore, analysis and determination of the content of the chemical compounds of Malacca leaves need to be done. This study was conducted to determine the content and identification of the ethanol extract compound of Malacca leaves macerated in three stages using n-hexane, ethyl acetate, and ethanol.


## 2. Materials and Methods

### 2.1. Malacca Leaf Samples

Malacca leaf samples were collected from Malacca plants that grew in the Blang Thutu area, Lambirah Village, Aceh Besar, Aceh, in April 2018. Identification of plants was conducted at the Herbarium Biology of Faculty of Mathematics and Natural Sciences, Syiah Kuala University, and identified as thePhyllanthus emblica Linn.


### 2.2. Malacca Leaf Sample Preparation

The extraction was carried out at Pharmacology Laboratory, Faculty of Veterinary Medicine, Syiah Kuala University. Malacca leaf samples were dried for several days, and then 1.5 kg dried samples were mashed to form simplisia. Simplisia was put into Erlenmeyer and then added with 1 liter of n-hexane. The maceration of simplisia was carried out for three days, and stirring was done infrequently. Subsequently, the maceration solution was filtered using the filter paper, and then the precipitate of simplisia was added with ethyl acetate followed by the maceration process for three days and filtration of the residue. The third maceration was performed using ethanol for three days, filtered, and dried using an evaporator until reaching paste-like texture.


### 2.3. Identification of Chemical Compounds by Using Gas Chromatography-Mass Spectrophotometer

Gas chromatography-mass spectrophotometer (GC-MS) used was GC-MS-QP2010S SHIMADZU consisting of the AOC-20i autosampler and gas chromatography connected to mass spectrometer instruments (GC-MS) using the following conditions: Restek RtxR-5 (30 meters × 0.25 mm) (5% diphenyl and 95% dimethyl polysiloxane) runs in the electron impact mode at 70 eV; helium (99.99%) was used as a carrier gas at a constant flow of 1 ml/minute, and an injection volume of 1.0μl was used (split ratio 10 : 1); and 300°C injector temperature. The oven temperature was programmed from 70°C (isothermal for 5 minutes), with an increase of 6°C/minute to 280°C and then terminated with isothermal for 15 minutes at 280°C. The mass spectrum was taken at 70 eV; 0.5 second scanning interval; and fragments from 40 to 550 Da. The total running time of GC is 60 minutes.The peaks that appear on the GC-MS screen were interpreted using the National Institute of Standard and Technology (NIST) database. The mass spectrum of unknown components was compared to the spectrum of known components stored in the NIST library.


## 2.1. Malacca Leaf Samples

Malacca leaf samples were collected from Malacca plants that grew in the Blang Thutu area, Lambirah Village, Aceh Besar, Aceh, in April 2018. Identification of plants was conducted at the Herbarium Biology of Faculty of Mathematics and Natural Sciences, Syiah Kuala University, and identified as thePhyllanthus emblica Linn.


## 2.2. Malacca Leaf Sample Preparation

The extraction was carried out at Pharmacology Laboratory, Faculty of Veterinary Medicine, Syiah Kuala University. Malacca leaf samples were dried for several days, and then 1.5 kg dried samples were mashed to form simplisia. Simplisia was put into Erlenmeyer and then added with 1 liter of n-hexane. The maceration of simplisia was carried out for three days, and stirring was done infrequently. Subsequently, the maceration solution was filtered using the filter paper, and then the precipitate of simplisia was added with ethyl acetate followed by the maceration process for three days and filtration of the residue. The third maceration was performed using ethanol for three days, filtered, and dried using an evaporator until reaching paste-like texture.


## 2.3. Identification of Chemical Compounds by Using Gas Chromatography-Mass Spectrophotometer

Gas chromatography-mass spectrophotometer (GC-MS) used was GC-MS-QP2010S SHIMADZU consisting of the AOC-20i autosampler and gas chromatography connected to mass spectrometer instruments (GC-MS) using the following conditions: Restek RtxR-5 (30 meters × 0.25 mm) (5% diphenyl and 95% dimethyl polysiloxane) runs in the electron impact mode at 70 eV; helium (99.99%) was used as a carrier gas at a constant flow of 1 ml/minute, and an injection volume of 1.0μl was used (split ratio 10 : 1); and 300°C injector temperature. The oven temperature was programmed from 70°C (isothermal for 5 minutes), with an increase of 6°C/minute to 280°C and then terminated with isothermal for 15 minutes at 280°C. The mass spectrum was taken at 70 eV; 0.5 second scanning interval; and fragments from 40 to 550 Da. The total running time of GC is 60 minutes.The peaks that appear on the GC-MS screen were interpreted using the National Institute of Standard and Technology (NIST) database. The mass spectrum of unknown components was compared to the spectrum of known components stored in the NIST library.


## 3. Results and Discussion

Malacca leaf samples were collected from the Malacca plant which grew in Blang Thutu, Lambirah Village of Aceh Besar district (Figure1).Figure 1
Sampling location.The chemical compounds of the ethanolic extract of the Malacca leaf identified using GC-MS can be seen in Figure2. Structural analysis of organic compounds spectroscopically included using a mass spectrum to determine the molecular formula and to determine the mass of a molecule [24]; in addition to GCMS, there is also an infrared spectrophotometer to determine the functional group of a compound [25]. There were 22 chemical compounds identified, and the most abundant compound in the Malacca ethanolic extract of Malacca leaves was decanoic acid (22.93%).Figure 2
GC-MS chromatogram of the ethanolic extract of the leaves ofPhyllanthus emblica leaves.Octadecanoic acid was detected at 34.4 minutes in the ethanol extract of Malacca leaves and has another name stearic acid which has 18 carbon atom chain and saturated fatty acid that has a carbon backbone and consists of a long chain of carbon atoms bonded together by single bonds and with 2 hydrogen atoms attached to each internal carbon atom. The second largest compound detected at minute 37.9 was 9,12-octadecadinoic acid (14.99%). The third most detected compound at minute 38.4 was octadecanoic acid (7.59%), and the next minute was 9-hexadecenoic acid (6.17%), octadecanoic acid (5.95%), octadecanal (5.59%), 9,12-octadecanoic acid (5.06%), 3-eicosyne (4.75%), 1-hexadecenoic acid (4.08%), 11-tetradecen-1-ol (2.92%), 2-furanmethanol (2.83%), delta-guaiene (2.43%), cyclohexane (2.13%), hexadecenoic acid (1.99%), sativen (1.87%), octadecanoic acid (1.52%), 1H-cyclopropaanaphthalene (1.40%), tetradecanoic acid (1.40%), 3,7,11-tridecatrienenitrile (1.20%), caryophellene (1.11%), 2H-pyran (1.07%), and minute 23.4 found trans-caryophellene (1.03%) (Table1).Table 1
Phytocomponents identified from the ethanol extract ofPhyllanthus emblica leaves.
Peak no.
Retention time
Name of the compound
Molecular formula
Mol weight
Peak area %
1
12,949
2-Furanmethanol
C5 H6 O2
98
2.83
2
21,551
1H-Cyclopropaanaphthalene
C15 H24
204
1.40
3
22,514
Trans-caryophyllene
C15H24
204
1.03
4
22,874
Cyclohexane
C13H20
176
2.13
5
23,402
Caryophyllene
C15H24
204
1.11
6
23,402
Sativen
C15H24
204
1.87
7
23,794
Delta-guaiene
C15H24
204
2.43
8
24,695
Tetradecanoic acid
C15H30O2
242
1.40
9
32,500
Octadecanal
C18H36O
268
5.59
10
33,428
3-Eicosyne
C20H38
278
4.75
11
34,402
Octadecanoic acid
C19H38 O2
298
22.93
12
35,784
Octadecanoic acid
C20H40 O2
312
5.95
13
36,444
Octadecanoic acid
C19H38 O2
298
1.52
14
37,792
9,12-Octadecadienoic acid
C19H34 O2
294
5.06
15
37,921
9,12-Octadecadienoic acid
C19H34 O2
294
14.99
16
38,414
Octadecanoic acid
C19H38 O2
298
7.59
17
39,184
9-Hexadecenoic acid
C16H30 O2
254
6.17
18
39,304
1-Hexadecanol
C16H34O
242
4.08
19
39,668
Hexadecenoic acid
C18H36 O2
284
1.99
20
40,034
11-Tetradecen-1-ol
C16H30 O2
254
2.92
21
49,859
3,7,11-Tridecatrienenitrile
C16H25N
231
1.20
22
54,548
2H-Pyran
C22H40 O2
336
1.07Some types of medicinal plants that contain a lot of fatty acids are very good as antioxidants and antimalarial. The active ingredients detected have very little detailed information available explaining the function of each of them. Another name for tetradecanoic acid is myristic acid, the dodecanoic acid synonym is lauric acid, and the synonym of octadecanoic acid is stearic acid; the whole chemical component is included in the group of saturated fatty acids [26].


## 4. Conclusions

The GC-MS analysis of the Malacca leaf extract (Phyllanthus emblica) clearly shows the presence of fatty acids which are mainly found in the leaf. There were 22 chemical compounds identified, and the most abundant compound in the Malacca ethanolic extract of Malacca leaves was decanoic acid (22.93%). These chemical constituents play a major role in the efficacy of these traditional medicines particularly as antioxidants and antimalarial. The study also showed that many other phytochemical compounds have not been tested for their biological activity. Therefore, further research is needed to be carried out on the application of these phytochemical compounds in pharmaceutical fields.



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*Source: 2739056-2020-04-27.xml*