# Identification of NF-κB Modulation Capabilities within Human Intestinal Commensal Bacteria

Authors: Omar Lakhdari; Julien Tap; Fabienne Béguet-Crespel; Karine Le Roux; Tomas de Wouters; Antonietta Cultrone; Malgorzata Nepelska; Fabrice Lefèvre; Joël Doré; Hervé M. Blottière
Journal: Journal of Biomedicine and Biotechnology
 (2011)

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## Abstract

The intestinal microbiota plays an important role in modulation of mucosal immune responses. To seek interactions between intestinal epithelial cells (IEC) and commensal bacteria, we screened 49 commensal strains for their capacity to modulate NF-κB. We used HT-29/kb-seap-25 and Caco-2/kb-seap-7 intestinal epithelial cells and monocyte-like THP-1 blue reporter cells to measure effects of commensal bacteria on cellular expression of a reporter system for NF-κB. Bacteria conditioned media (CM) were tested alone or together with an activator of NF-κB to explore its inhibitory potentials. CM from 8 or 10 different commensal species activated NF-κB expression on HT-29 and Caco-2 cells, respectively. On THP-1, CM from all but 5 commensal strains stimulated NF-κB. Upon challenge with TNF-α or IL-1β, some CM prevented induced NF-κB activation, whereas others enhanced it. Interestingly, the enhancing effect of some CM was correlated with the presence of butyrate and propionate. Characterization of the effects of the identified bacteria and their implications in human health awaits further investigations.


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## Body

## 1. Introduction

The adult human gut is populated with a large number of commensal bacteria known to influence many aspects of the host gut physiology, including immunity, development, and homeostasis. There is considerable clinical and experimental evidence showing that commensal gut bacteria contribute to immune homeostasis by altering microbial balance or by interacting with the gut immune system [1]. However, cellular and molecular mechanisms by which individual members of the commensal microbiota contribute to immune homeostasis have not been completely elucidated.Intestinal epithelial cells (IEC) are the first point of contact for bacteria within the gut lumen, and they interact with the gut immune system. Consequently, IEC have a pivotal function in bacteria-host communication. Bacterial signatures generally activate signaling cascades that can trigger proinflammatory gene transcription through specific receptors (e.g., Toll-like receptors) expressed on apical and/or basolateral surface of epithelial cells. This mechanism is largely controlled by the transcriptional factor NF-κB [2]. NF-κB is a dimeric DNA binding protein whose major form is represented by the association of p65 and p50 proteins. In steady state, NF-κB is locked in the cytoplasm by an inhibitory protein of the IκB family. Upon receptor activation, IκB is phosphorylated by the IκB kinase complex (IKK) before undergoing degradation by the proteasome. Then, free NF-κB translocate to the nucleus to turn on a large number of genes involved in proinflammatory processes at the site of infection or tissue damage.Obviously, the intestinal epithelium does not trigger inflammatory responses against commensal bacteria. Interestingly, the mechanisms allowing commensal micro-organisms to be tolerated by the intestinal mucosa are far from being completely understood. Numerous studies have suggested an involvement of active processes causing a functional downregulation of the inflammatory response that is generally obtained by interference with the NF-κB pathway. Indeed, previous reports showed the suppression of inflammatory responses in epithelial cells by commensal bacteria either through direct interaction leading to alteration in Toll-like receptors (TLRs) signaling to NF-κB    [3, 4] or through direct inhibition of NF-κB transcriptional activity    [5]. In addition, some secreted bacterial factors resulting from commensals or probiotics activity have been found to exert anti-inflammatory effects on IEC    [6–10].Furthermore, recent work has ascribed a critical role for NF-κB signaling in maintenance of homeostatic immuno-inflammatory function in the gut [11]. Indeed epithelial NF-κB preserves integrity of the gut epithelial barrier and coordinates antimicrobial actions of the innate and adaptive immune systems. Accordingly, both deficiency in, and hyperactivation of, this transcription factor are underlying factors in chronic inflammatory bowel diseases    [12–16].Finally, determining the microbial factors that positively or negatively regulate this key pathway is of great clinical and scientific interest. Thus, in the present study, we examined the effect of 49 commensal bacteria on the modulation of NF-κB pathway in human IEC models bearing a stable NF-κB-SEAP reporter system. Conditioned media (CM) from these bacteria were tested either alone or in combination with an activator of NF-κB signaling (TNF-α or IL-1β) to identify its inhibitory and enhancing potentials. To compare response profiles between IEC and immune cells, all CM were also tested on human monocyte cell line THP-1 bearing the same NF-κB reporter system.


## 2. Material and Methods

### 2.1. Cell Culture and Reagents

HT-29 cells were grown in DMEM (Sigma) with 2 mM L-glutamine, 50 IU/mL penicillin, 50μg/mL streptomycin and 10% heat-inactivated fetal calf serum (FCS-Lonza) in a humidified 5% CO2 atmosphere at 37°C. Caco-2 were cultured in DMEM (Sigma) supplemented with the same amounts of glutamine and antibiotics, 20% heat-inactivated FCS and 1x nonessential amino acids (Invitrogen). The THP-1 blue CD14+ NF-κB reporter clone was obtained from Invivogen and used according to the manufacturer’s instruction.


### 2.2. Commensal Strains and Preparation of Conditioned Media

The 49 commensal strains were grown in anaerobic condition at 37°C using the Hungate culture method [17]. Screened strains and corresponding growth media are listed in Table 1.Table 1
Bacteria strains, references, and growth media.
DesignationCollection referenceMediumPhylumAtopobium parvulumDSM 20649TM104ABifidobacterium adolescentisATCC 15703M58ABifidobacterium angulatumATCC 27535-CIP 104167M58ABifidobacterium animalis ssp. animalisDSM 20104M58ABifidobacterium bifidumDSM20082/JCM1254M58ABifidobacterium breve 1DSMZ 20091M104ABifidobacterium breve 2ATCC 15701-89/23M104ABifidobacterium catenulatumATCC27539-CIP104175M58ABifidobacterium choerinumDSM 20434M58ABifidobacterium dentium 1ATCC 15423M104ABifidobacterium dentium 2ATCC 27534-89/20M104ABifidobacterium gallicumATCC 49850-CIP 103417M58ABifidobacterium infantisDSM20088/ATCC15697M58ABifidobacterium longumATCC 15707-NCTC 11818M58ABifidobacterium pseudocatenulatumDSM 20438-ATCC 27919M58ABifidobacterium ruminantiumATCC 49390M58ACollinsella aerofaciensATCC 25986BHI**APropionibacterium acnesB15M104ABacteroides caecaeATCC 43185-CIP 104201TBHIBBacteroides doreiDSM 17855M104BBacteroides fragilisB6-AL 2553BHIBBacteroides ovatusATCC 8483-CIP103756M104BBacteroides thetaiotaomicronATCC 29148-VPI5482M104BBacteroides uniformisATCC  8492M104BBacteroides vulgatusATCC 8482-CIP 103714BHIBParabacteroides distasonisCIP104284-ATCC8503BHIBParabacteroides johnsoniiDSM 18315M104BPrevotella copriDSM 18205M104BBlautia/Clostridium coccoidesATCC 2936BHIFBlautia producta/Ruminococcus productusDSMZ 2950-92/46M104FBlautia/Ruminococcus hanseniiDSM 20583M104FButyrivibrio fibrisolvensDSM3071BHIFClostridium leptumATCC29065-DSM753BHIFClostridium nexile 96/2ATCC 27757-DSM 1787BHIFClostridium paraputrificumG12PR-X73445BHIFClostridium sardiniensisI11PRM78FClostridium sordelliiATCC 9714BHIFClostridium sporosphaeroidesATCC 25781M78FDorea/Eubacterium formicigeneransATCC 27755L-DON*FEubacterium rectaleATCC 33656-CIP105953BHIFFaecalibacterium prausnitziiL2-6 (Duncan)BHIFRoseburia faecisDSM 16840BHIFRoseburia hominisDSM 16839M58FRoseburia intestinalisDSM 14610M58FRuminococcus gnavusFRE1L-DONFRuminococcus lactarisATCC 29176L-DONFRuminococcus obeumATCC 29174L-DONFRuminococcus torquesATCC 27756M104FSelenomonas ruminantiumATCC19205-DSM2872L-DONF*Yeast extract-CaCl2—sodium thioglycholate—pyruvic acid.**Brain heart infusion + yeast extract and hemine.A: Actinobacteria; B: Bacteroidetes; F: Firmicutes.At the end of the incubation period, bacterial cultures were centrifuged at 5,000 xg for 10 minutes. Bacteria conditioned media (CM) were then collected and filtered on 0.2μm PES filters. Noninoculated bacteria culture medium served as control.


### 2.3. Analyses of NF-κB Activation—SEAP Reporter Assay

Construction and validation of the NF-κB reporter clones HT-29/kb-seap-25 and Caco-2/kb-seap-7 have been described previously [18]. For each experiment, Caco-2/kb-seap-7 and HT-29/kb-seap-25 reporter clones were seeded at 50,000 cells per well, into 96-wells plates and incubated 24 hours. Then, cells were stimulated for 24 hours with 10 μL of each tested bacteria CM, for a final volume of 100 μL per well (i.e., 10% vol/vol), in the presence or absence of TNF-α or IL-1β (10 ng/mL final, for HT-29 and Caco-2, resp.).THP-1 reporter cells were seeded at 100,000 cells per well, into 96-wells plates and stimulated with 10% (vol/vol) of each tested bacteria CM. Cells were then incubated 24 hours prior to quantification of alkaline phosphatase (SEAP).SEAP in the supernatant was revealed using the Quanti-Blue reagent (Invivogen) according to the manufacturer’s protocol and quantified at 655 nm OD. All measurements were performed using a microplate reader (Infinite 200, Tecan).


### 2.4. Statistical Analysis

Results are expressed as mean ± SD. Data were analyzed using Student’st test.


## 2.1. Cell Culture and Reagents

HT-29 cells were grown in DMEM (Sigma) with 2 mM L-glutamine, 50 IU/mL penicillin, 50μg/mL streptomycin and 10% heat-inactivated fetal calf serum (FCS-Lonza) in a humidified 5% CO2 atmosphere at 37°C. Caco-2 were cultured in DMEM (Sigma) supplemented with the same amounts of glutamine and antibiotics, 20% heat-inactivated FCS and 1x nonessential amino acids (Invitrogen). The THP-1 blue CD14+ NF-κB reporter clone was obtained from Invivogen and used according to the manufacturer’s instruction.


## 2.2. Commensal Strains and Preparation of Conditioned Media

The 49 commensal strains were grown in anaerobic condition at 37°C using the Hungate culture method [17]. Screened strains and corresponding growth media are listed in Table 1.Table 1
Bacteria strains, references, and growth media.
DesignationCollection referenceMediumPhylumAtopobium parvulumDSM 20649TM104ABifidobacterium adolescentisATCC 15703M58ABifidobacterium angulatumATCC 27535-CIP 104167M58ABifidobacterium animalis ssp. animalisDSM 20104M58ABifidobacterium bifidumDSM20082/JCM1254M58ABifidobacterium breve 1DSMZ 20091M104ABifidobacterium breve 2ATCC 15701-89/23M104ABifidobacterium catenulatumATCC27539-CIP104175M58ABifidobacterium choerinumDSM 20434M58ABifidobacterium dentium 1ATCC 15423M104ABifidobacterium dentium 2ATCC 27534-89/20M104ABifidobacterium gallicumATCC 49850-CIP 103417M58ABifidobacterium infantisDSM20088/ATCC15697M58ABifidobacterium longumATCC 15707-NCTC 11818M58ABifidobacterium pseudocatenulatumDSM 20438-ATCC 27919M58ABifidobacterium ruminantiumATCC 49390M58ACollinsella aerofaciensATCC 25986BHI**APropionibacterium acnesB15M104ABacteroides caecaeATCC 43185-CIP 104201TBHIBBacteroides doreiDSM 17855M104BBacteroides fragilisB6-AL 2553BHIBBacteroides ovatusATCC 8483-CIP103756M104BBacteroides thetaiotaomicronATCC 29148-VPI5482M104BBacteroides uniformisATCC  8492M104BBacteroides vulgatusATCC 8482-CIP 103714BHIBParabacteroides distasonisCIP104284-ATCC8503BHIBParabacteroides johnsoniiDSM 18315M104BPrevotella copriDSM 18205M104BBlautia/Clostridium coccoidesATCC 2936BHIFBlautia producta/Ruminococcus productusDSMZ 2950-92/46M104FBlautia/Ruminococcus hanseniiDSM 20583M104FButyrivibrio fibrisolvensDSM3071BHIFClostridium leptumATCC29065-DSM753BHIFClostridium nexile 96/2ATCC 27757-DSM 1787BHIFClostridium paraputrificumG12PR-X73445BHIFClostridium sardiniensisI11PRM78FClostridium sordelliiATCC 9714BHIFClostridium sporosphaeroidesATCC 25781M78FDorea/Eubacterium formicigeneransATCC 27755L-DON*FEubacterium rectaleATCC 33656-CIP105953BHIFFaecalibacterium prausnitziiL2-6 (Duncan)BHIFRoseburia faecisDSM 16840BHIFRoseburia hominisDSM 16839M58FRoseburia intestinalisDSM 14610M58FRuminococcus gnavusFRE1L-DONFRuminococcus lactarisATCC 29176L-DONFRuminococcus obeumATCC 29174L-DONFRuminococcus torquesATCC 27756M104FSelenomonas ruminantiumATCC19205-DSM2872L-DONF*Yeast extract-CaCl2—sodium thioglycholate—pyruvic acid.**Brain heart infusion + yeast extract and hemine.A: Actinobacteria; B: Bacteroidetes; F: Firmicutes.At the end of the incubation period, bacterial cultures were centrifuged at 5,000 xg for 10 minutes. Bacteria conditioned media (CM) were then collected and filtered on 0.2μm PES filters. Noninoculated bacteria culture medium served as control.


## 2.3. Analyses of NF-κB Activation—SEAP Reporter Assay

Construction and validation of the NF-κB reporter clones HT-29/kb-seap-25 and Caco-2/kb-seap-7 have been described previously [18]. For each experiment, Caco-2/kb-seap-7 and HT-29/kb-seap-25 reporter clones were seeded at 50,000 cells per well, into 96-wells plates and incubated 24 hours. Then, cells were stimulated for 24 hours with 10 μL of each tested bacteria CM, for a final volume of 100 μL per well (i.e., 10% vol/vol), in the presence or absence of TNF-α or IL-1β (10 ng/mL final, for HT-29 and Caco-2, resp.).THP-1 reporter cells were seeded at 100,000 cells per well, into 96-wells plates and stimulated with 10% (vol/vol) of each tested bacteria CM. Cells were then incubated 24 hours prior to quantification of alkaline phosphatase (SEAP).SEAP in the supernatant was revealed using the Quanti-Blue reagent (Invivogen) according to the manufacturer’s protocol and quantified at 655 nm OD. All measurements were performed using a microplate reader (Infinite 200, Tecan).


## 2.4. Statistical Analysis

Results are expressed as mean ± SD. Data were analyzed using Student’st test.


## 3. Results

### 3.1. Effect of Bacteria CM on NF-κB Activation in IEC and Monocyte Models

Out of 49 bacteria CM, 8 and 10 CM significantly activated the NF-κB reporter system on HT-29 and Caco-2 reporter cells, respectively (Figures 1(a) and 1(b)). In fact, the 2 cell lines were largely unresponsive to the vast majority of the tested bacteria CM. Active bacteria CM identified on both epithelial cells belonged to Clostridium sardiniensis, Selenomonas ruminantium, Roseburia hominis, Roseburia intestinalis, Butyrivibrio fibrisolvens, Roseburia faecis, and Faecalibacterium prausnitzii. Bacteroides uniformis activated NF-κB only on HT-29/kb-seap-25, while a small, nonsignificant stimulation was observed on Caco-2 (P>.05). Clostridium paraputrificum and Parabacteroides distasonis induced NF-κB activation specifically in Caco-2/kb-seap-7. Although statistically significant, these observed stimulations were lower than the one observed with IL-1β on Caco-2/kb-seap-7 cells (mean 1.03 ± 0.17) or with TNF-α on HT-29/kb-seap-25 (mean 0.92±0.18).Comparison of the stimulatory effect of 49 commensal bacteria conditioned media on HT-29/kb-seap-25, Caco-2/kb-seap-7, and THP-1-blue. NF-κB activity is expressed as OD 655 nm.  Data are presented as mean +/− SD of 3 independent experiments performed in HT-29 (a) and Caco-2 (b) and of 2 independent experiments performed in THP-1 (c). Control is noninoculated bacteria medium and its activity was normalized to 0 (represented by the X-axis). *P<.05 compared to control. For THP-1 only: ns: not significant. All other values are significantly different from control (P<.05).
(a)(b)(c)Conversely, almost all bacteria CM activated NF-κB on THP-1 blue reporter cells except those from Ruminococcus gnavus, Ruminococcus obeum, andRuminococcus lactaris(Figure 1(c)). Stimulation levels measured in HT-29 and Caco-2 were weak in comparison to those obtained in THP-1. Lipopolysaccharide (LPS) used as control in THP-1 cells showed a lower stimulatory effect than most activating CM (mean 0.38 ± 0.13).


### 3.2. Effect of Bacteria CM on Activated NF-κB in IEC

In order to explore the inhibitory and/or enhancing potentials of commensal bacteria CM on NF-κB activation, cotreatment with the proinflammatory cytokines TNF-α (10 ng/mL) or IL-1β (10 ng/mL) was performed on HT-29 and Caco-2, respectively (Figure 2). The positive control of NF-κB activation was noninoculated bacteria culture medium combined with the stimulatory cytokine. The value of NF-κB activity measured for the positive control was normalized to 0.  The values obtained for each strain were expressed as percentage of activation relative to that of the positive control. For example, Selenomonas ruminantium CM combined with TNF-α induced NF-κB activity 21.6% higher than the one obtained with the positive control (i.e., noninoculated bacteria medium combined with TNF-α).(a) Effect of bacteria CM on activated NF-κB in HT-29 and Caco-2 reporter cells. NF-κB activity is expressed as a relative percentage compared to the positive control (normalized to 0). Positive controls are cells treated with noninoculated bacteria culture medium and the stimulatory cytokine. The corresponding P value is indicated in parenthesis and inhibitory and stimulatory strains are identified following the color code shown in (b).
(a)(b)A different behavior of the 2 epithelial models in response to the bacteria CM could also be observed (Figure2). Indeed on HT-29, CM overall enhanced NF-κB activity more than in Caco-2. Interestingly, a large majority of Bifidobacteria CM were stimulatory on HT-29 while inhibitory on Caco-2.Some bacteria CM had a similar effect on the 2 reporter cells (Figure2). Induced NF-κB activation was restrained in the 2 cell lines only by Colinsella aerofasciens, with inhibition rates of 14.1% (P=.081) and 17.1% (P=.027) for HT-29 and Caco-2, respectively. Furthermore, the 3 species of Roseburiaas well as Clostridium sardiniensis, Clostridium sporosphareoides, and Bacteroides uniformis enhanced NF-κB activation significantly in both cell lines.We also observed that some CM had effects only on one reporter cell line such as CM fromSelenomonas ruminantium, Faecalibacterium prauznitzii, Bacteroides fragilis, Parabacteroides distasonis, and Bifidobacterium breve 1. These CM increased NF-κB activity in HT-29/kb-seap-25 but exhibited nonsignificant effects on Caco-2/kb-seap-7. Similarly, Eubacterium rectale, Clostridium nexile 96/2, Clostridium  leptum, Blautia  coccoides, andBacteroides vulgatusexerted significant inhibitory effects only on Caco-2.


### 3.3. Effect of Short Chain Fatty and Organic Acids on NF-κB Activation in IEC

Commensal bacteria are known to produce a panel of acids during their metabolic activity, especially short chain fatty acid (SCFA), which could interfere with NF-κB response.Therefore, we evaluated the effects of acetic, butyric, propionic, lactic, succinic, and formic acids on NF-κB in IEC, either alone or on cytokine-activated cells (Figure 3). The results presented were obtained on HT-29 although similar observations were performed on Caco-2 (data not shown).Effect of SCFA and organic acids on NF-κB activation. HT-29 cells were treated with acetic, butyric, propionic, lactic, succinic, and formic acids either alone or in combination with TNF-α (10 ng/mL) for 24 hours. Results are expressed as OD 655 nm. Data is representative of 1 experiment out of 3 performed. *P<.05 compared to TNF control.
(a)(b)None of the acids had an effect on HT-29 or Caco-2 when used alone. However, butyrate and propionate produced a dose-dependent hyperactivation of NF-κB on TNF-α activated cell. The other acids induced a small but significant stimulatory effect only at the highest concentration (6–8 mM final) and a very small but yet significant inhibitory effect of NF-κB at the lowest concentrations. Since butyrate and propionate are likely to act on activated NF-κB signaling, we quantified SCFA in the CM by HPLC and examined their associations with NF-κB activity. We found that out of 49 bacteria CM, 19 contained butyrate, propionate, or both (Figure 4).Correlation between butyrate or propionate concentration of the bacteria CM and NF-κB activity. NF-κB activity measured in HT-29 in response to cotreatment with bacteria CM and TNF-α (10 ng/mL) based on the amount of butyrate (a) and propionate (b) present in each bacteria CM. NF-κB activity is expressed as a relative percentage (values from Figure 2). Butyrate and propionate amounts were determined by HPLC and expressed in mM. A Spearman correlation test was performed: Butyrate versus NF-κB activity: r=0.76 (P=.036). Propionate versus NF-κB activity: r=0.49 (P=.075). Propionate (Bacteroidetes only) versus NF-κB activity: r=0.81 (P=.005).
(a)(b)Figure4 represents a plot of the amount of butyrate (Figure 4(a)) or propionate (Figure 4(b)) present in each bacteria CM (X-axis) with the NF-κB activity measured in HT-29 in response to cotreatment with bacteria CM and TNF-α (see Figure 2).  A Spearman correlation test was performed by taking into account butyrate and propionate concentrations greater than 1 mM. A significant positive correlation (r=0.76, P=.036) was observed between butyrate concentration and NF-κB activity, suggesting that the butyrate-producing strains might have exerted their stimulatory effect through the butyrate released during growth. Similarly, most of the propionate-producing bacteria were also stimulatory on HT-29, but the correlation between propionate concentration and NF-κB activity was not significant (r=0.49, P=.075). However, the correlation became highly significant when only strains from the Bacteroidetes phylum (r=0.81, P=.005) were included in the analyses. This suggests that the effect of the other propionate-producing strains might be due to other active metabolites that are different from propionate.


## 3.1. Effect of Bacteria CM on NF-κB Activation in IEC and Monocyte Models

Out of 49 bacteria CM, 8 and 10 CM significantly activated the NF-κB reporter system on HT-29 and Caco-2 reporter cells, respectively (Figures 1(a) and 1(b)). In fact, the 2 cell lines were largely unresponsive to the vast majority of the tested bacteria CM. Active bacteria CM identified on both epithelial cells belonged to Clostridium sardiniensis, Selenomonas ruminantium, Roseburia hominis, Roseburia intestinalis, Butyrivibrio fibrisolvens, Roseburia faecis, and Faecalibacterium prausnitzii. Bacteroides uniformis activated NF-κB only on HT-29/kb-seap-25, while a small, nonsignificant stimulation was observed on Caco-2 (P>.05). Clostridium paraputrificum and Parabacteroides distasonis induced NF-κB activation specifically in Caco-2/kb-seap-7. Although statistically significant, these observed stimulations were lower than the one observed with IL-1β on Caco-2/kb-seap-7 cells (mean 1.03 ± 0.17) or with TNF-α on HT-29/kb-seap-25 (mean 0.92±0.18).Comparison of the stimulatory effect of 49 commensal bacteria conditioned media on HT-29/kb-seap-25, Caco-2/kb-seap-7, and THP-1-blue. NF-κB activity is expressed as OD 655 nm.  Data are presented as mean +/− SD of 3 independent experiments performed in HT-29 (a) and Caco-2 (b) and of 2 independent experiments performed in THP-1 (c). Control is noninoculated bacteria medium and its activity was normalized to 0 (represented by the X-axis). *P<.05 compared to control. For THP-1 only: ns: not significant. All other values are significantly different from control (P<.05).
(a)(b)(c)Conversely, almost all bacteria CM activated NF-κB on THP-1 blue reporter cells except those from Ruminococcus gnavus, Ruminococcus obeum, andRuminococcus lactaris(Figure 1(c)). Stimulation levels measured in HT-29 and Caco-2 were weak in comparison to those obtained in THP-1. Lipopolysaccharide (LPS) used as control in THP-1 cells showed a lower stimulatory effect than most activating CM (mean 0.38 ± 0.13).


## 3.2. Effect of Bacteria CM on Activated NF-κB in IEC

In order to explore the inhibitory and/or enhancing potentials of commensal bacteria CM on NF-κB activation, cotreatment with the proinflammatory cytokines TNF-α (10 ng/mL) or IL-1β (10 ng/mL) was performed on HT-29 and Caco-2, respectively (Figure 2). The positive control of NF-κB activation was noninoculated bacteria culture medium combined with the stimulatory cytokine. The value of NF-κB activity measured for the positive control was normalized to 0.  The values obtained for each strain were expressed as percentage of activation relative to that of the positive control. For example, Selenomonas ruminantium CM combined with TNF-α induced NF-κB activity 21.6% higher than the one obtained with the positive control (i.e., noninoculated bacteria medium combined with TNF-α).(a) Effect of bacteria CM on activated NF-κB in HT-29 and Caco-2 reporter cells. NF-κB activity is expressed as a relative percentage compared to the positive control (normalized to 0). Positive controls are cells treated with noninoculated bacteria culture medium and the stimulatory cytokine. The corresponding P value is indicated in parenthesis and inhibitory and stimulatory strains are identified following the color code shown in (b).
(a)(b)A different behavior of the 2 epithelial models in response to the bacteria CM could also be observed (Figure2). Indeed on HT-29, CM overall enhanced NF-κB activity more than in Caco-2. Interestingly, a large majority of Bifidobacteria CM were stimulatory on HT-29 while inhibitory on Caco-2.Some bacteria CM had a similar effect on the 2 reporter cells (Figure2). Induced NF-κB activation was restrained in the 2 cell lines only by Colinsella aerofasciens, with inhibition rates of 14.1% (P=.081) and 17.1% (P=.027) for HT-29 and Caco-2, respectively. Furthermore, the 3 species of Roseburiaas well as Clostridium sardiniensis, Clostridium sporosphareoides, and Bacteroides uniformis enhanced NF-κB activation significantly in both cell lines.We also observed that some CM had effects only on one reporter cell line such as CM fromSelenomonas ruminantium, Faecalibacterium prauznitzii, Bacteroides fragilis, Parabacteroides distasonis, and Bifidobacterium breve 1. These CM increased NF-κB activity in HT-29/kb-seap-25 but exhibited nonsignificant effects on Caco-2/kb-seap-7. Similarly, Eubacterium rectale, Clostridium nexile 96/2, Clostridium  leptum, Blautia  coccoides, andBacteroides vulgatusexerted significant inhibitory effects only on Caco-2.


## 3.3. Effect of Short Chain Fatty and Organic Acids on NF-κB Activation in IEC

Commensal bacteria are known to produce a panel of acids during their metabolic activity, especially short chain fatty acid (SCFA), which could interfere with NF-κB response.Therefore, we evaluated the effects of acetic, butyric, propionic, lactic, succinic, and formic acids on NF-κB in IEC, either alone or on cytokine-activated cells (Figure 3). The results presented were obtained on HT-29 although similar observations were performed on Caco-2 (data not shown).Effect of SCFA and organic acids on NF-κB activation. HT-29 cells were treated with acetic, butyric, propionic, lactic, succinic, and formic acids either alone or in combination with TNF-α (10 ng/mL) for 24 hours. Results are expressed as OD 655 nm. Data is representative of 1 experiment out of 3 performed. *P<.05 compared to TNF control.
(a)(b)None of the acids had an effect on HT-29 or Caco-2 when used alone. However, butyrate and propionate produced a dose-dependent hyperactivation of NF-κB on TNF-α activated cell. The other acids induced a small but significant stimulatory effect only at the highest concentration (6–8 mM final) and a very small but yet significant inhibitory effect of NF-κB at the lowest concentrations. Since butyrate and propionate are likely to act on activated NF-κB signaling, we quantified SCFA in the CM by HPLC and examined their associations with NF-κB activity. We found that out of 49 bacteria CM, 19 contained butyrate, propionate, or both (Figure 4).Correlation between butyrate or propionate concentration of the bacteria CM and NF-κB activity. NF-κB activity measured in HT-29 in response to cotreatment with bacteria CM and TNF-α (10 ng/mL) based on the amount of butyrate (a) and propionate (b) present in each bacteria CM. NF-κB activity is expressed as a relative percentage (values from Figure 2). Butyrate and propionate amounts were determined by HPLC and expressed in mM. A Spearman correlation test was performed: Butyrate versus NF-κB activity: r=0.76 (P=.036). Propionate versus NF-κB activity: r=0.49 (P=.075). Propionate (Bacteroidetes only) versus NF-κB activity: r=0.81 (P=.005).
(a)(b)Figure4 represents a plot of the amount of butyrate (Figure 4(a)) or propionate (Figure 4(b)) present in each bacteria CM (X-axis) with the NF-κB activity measured in HT-29 in response to cotreatment with bacteria CM and TNF-α (see Figure 2).  A Spearman correlation test was performed by taking into account butyrate and propionate concentrations greater than 1 mM. A significant positive correlation (r=0.76, P=.036) was observed between butyrate concentration and NF-κB activity, suggesting that the butyrate-producing strains might have exerted their stimulatory effect through the butyrate released during growth. Similarly, most of the propionate-producing bacteria were also stimulatory on HT-29, but the correlation between propionate concentration and NF-κB activity was not significant (r=0.49, P=.075). However, the correlation became highly significant when only strains from the Bacteroidetes phylum (r=0.81, P=.005) were included in the analyses. This suggests that the effect of the other propionate-producing strains might be due to other active metabolites that are different from propionate.


## 4. Discussion

In this study, we aimed at identifying commensal strains deemed capable of regulating eukaryotic cell signaling focusing on the NF-κB signaling pathway, which is largely involved in immune and inflammatory responses.In the IEC models HT-29 and Caco-2, the majority of bacteria CM had no effect on NF-κB, contrasting with the results obtained with the monocyte cell line THP-1. This observation may be explained by the differences in the expression of receptors specialized in recognition of microbial structures, especially TLRs. Indeed, THP-1 reporter clone as well as the parental cell line express a complete range of TLRs and all five surface TLRs (TLR1/2, TLR2, TLR6/2, TLR4, and TLR5) are present and functional    [18–20], resulting in increased sensitivity to a wide range of microbe-associated molecular patterns. Conversely, HT-29 and Caco-2 do not express all TLRs. Parental HT-29 cell line as well as the reporter clone HT-29/kb-seap-25 mainly express a functional TLR5, which is responsible for detection of flagellin    [21]. Caco-2 parental cell line was also described to be responsive to TLR5 stimulation    [22, 23], but the reporter clone Caco-2/kb-seap-7 that was obtained and used throughout our study is poorly sensitive [18]. In addition, unresponsiveness toward stimulation of other TLRs such as TLR2 and TLR4 was reported for parental intestinal epithelial cell lines    [24–26] and was also observed with our IEC reporter clones [18].Furthermore, the fact that HT-29/kb-seap-25 is sensitive to flagellin explains the basal stimulatory effect displayed by the CM from flagellated commensal strains such asRoseburia spp., Selenomonas ruminantium and Butyrivibrio fibrisolvens    [27–29]. These flagellated commensals also stimulated NF-κB in Caco-2/kb-seap-7, but these cells are less sensitive to flagellin than HT-29 which explains the weak response obtained. Stimulation by Bacteroides uniformis and Faecalibacterium prausnitzii CM on both cell lines was not likely due to a putative flagellin expression since these bacteria are not motile    [30, 31], which is not the case with Clostridium sardiniensis    [32].Clostridium paraputrificum, Parabacteroides distasonis, and Bifidobacterium breve 1 CM stimulated Caco-2 but not HT-29, supporting  the assertion that the effect is independent of TLR5 recognition. Since Caco-2 reporter cells are not sensitive to TLRs stimulation [18], the specific stimulatory effect of these 3 bacteria CM need further evaluation. In addition, the lack of response of THP-1 toward CM from 3 different strains of Ruminococcus is an intriguing observation that also deserves attention.The 2 reporter cells used in this study were stimulated by the commensal bacteria CM combined with a known NF-κB activator. This challenge was performed in order to identify both the stimulatory as well as the inhibitory contributors of NF-κB signaling activity. The 2 proinflammatory cytokines TNF-α and IL-1β were used to activate NF-κB on HT-29 and Caco-2, respectively, [18]. Under these conditions, some CM remarkably dampened NF-κB activation in these cells with the inhibition occurring mainly in IL-1β-activated Caco-2.SeveralBifidobacterium strains were described for their capacity to dampen NF-κB activation in vitro in different cell models, including Caco-2 and HT-29    [33, 34]. Consistent with this,   few Bifidobacterium CM reduced NF-κB activation on Caco-2, but were ineffective on HT-29. In contrast, in the present study, CM from Bifidobacteriumstrains enhanced NF-κB activation by TNF on HT-29. However, in our study, we measured the transcriptional activity of NF-κB instead of the product of one gene controlled by NF-κB. It is well known that NF-κB controls the activation of several genes not only involved in inflammatory processes, but also in tissue protection and homeostasis. For example, production of human β-defensin in IEC, which is NF-κB-dependent    [35], is stimulated by probiotics, including the Bifidobacterium-containing mixture VSL#3    [36]. Obviously, this dual effect of Bifidobacterium strains on NF-κB signaling requires further examination.Butyrate (or butyric acid) and propionate, 2 products of bacterial fermentation, enhanced NF-κB activation induced by TNF-α or IL-1β in our reporter cells. As such, butyrate-producing bacteria stimulated NF-κB activity and a strong correlation has been found between bacteria CM butyrate concentration and NF-κB activity.However some propionate-producing bacteria, such asEubacterium rectale and Clostridium leptum did not enhance NF-κB, suggesting the existence of other metabolites that may counter the stimulatory effect of propionate on activated epithelial cells.It is noteworthy that although butyrate is classically known for preventing NF-κB activation in IEC    [37–39], some recent studies suggest that butyrate also promotes NF-κB transcriptional activity in IEC    [40, 41]. In addition, butyrate has been shown to promote human β-defensin expression, whose gene transcription is controlled by NF-κB    [42].


## 5. Conclusion

The mechanisms underlying the inhibitory and stimulatory effects of NF-κB signaling in monocyte and IEC models by nonbutyrate-producing and nonflagellated bacteria strains remain to be explored. The cell-based screening method employed in the present study provides a rapid identification of potentially interesting commensal species; however, their effects require further confirmation and characterization using other techniques of NF-κB detection. Moreover, the potential implication of these commensal bacteria and their host cells regulating properties in human health and disease may need to be evaluated.



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*Source: 282356-2011-06-14.xml*