# Radiation Sterilization of Anthracycline Antibiotics in Solid State

Authors: A. Kaczmarek; J. Cielecka-Piontek; P. Garbacki; K. Lewandowska; W. Bednarski; B. Barszcz; P. Zalewski; W. Kycler; I. Oszczapowicz; A. Jelińska
Journal: The Scientific World Journal
 (2013)

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## Abstract

The impact of ionizing radiation generated by a beam of electrons of 25–400 kGy on the stability of such analogs of anthracycline antibiotics as daunorubicin (DAU), doxorubicin (DOX), and epidoxorubicin (EPI) was studied. Based on EPR results, it was established that unstable free radicals decay exponentially with the half-time of 4 days in DAU and DOX and 7 days in EPI after irradiation. Radiation-induced structural changes were analyzed with the use of spectrophotometric methods (UV-Vis and IR) and electron microscope imaging (SEM). A chromatographic method (HPLC-DAD) was applied to assess changes in the contents of the analogs in the presence of their impurities. The study showed that the structures of the analogs did not demonstrate any significant alterations at the end of the period necessary for the elimination of unstable free radicals. The separation of main substances and related substances (impurities and potential degradation products) allowed determining that no statistically significant changes in the content of particular active substances occurred and that their conversion due to the presence of free radicals resulting from exposure to an irradiation of 25 kGy (prescribed to ensure sterility) was not observed.


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## Body

## 1. Introduction

Anthracycline antibiotics belong to the group of anticancer drugs. They were originally isolated from cultures ofStreptomyces. Anthracyclines are widely used in the treatment of neoplastic diseases such as leukemia, breast cancer, and AIDS-related Kaposi’s sarcoma. They also demonstrate activity against tumors of the ovaries, lung, testes, prostate, cervix, bladder, and Ewing’s sarcoma [1–3]. The most widely used anthracyclines are doxorubicin (DOX), daunorubicin (DAU), and epidoxorubicin (EPI) [4–6] (Figure 1). Their low bioavailability necessitates parenteral administration [7, 8], which requires sterility [9] obtained mainly by filtration. Given the considerable exposure of medical personnel to anthracycline antibiotics and their adsorption to most surfaces, especially those of sterilization filters, there is a need to find new sterilization methods that do not rely on filtration. Although radiation sterilization is an effective alternative, the structure of drugs may alter as a consequence of exposure to irradiation. The aglycone attached to the aminosugar with a glycoside bond may be prone to cleavage resulting from electron transfer within the molecule. Stability studies demonstrated degradation of anthracycline antibiotics in solutions, under the influence of increased temperature and when exposed to light in the solid state as well as a consequence of combining chemotherapy with radiotherapy [10–15]. Regarding the effects of radiation sterilization on anthracycline antibiotics, only DOX was studied in that respect with a focus on the impact of a standard dose of 25 kGy on its stability [16]. As DAU and EPI are widely used in anticancer pharmacotherapy, those antibiotics also require analysis of their vulnerability to ionizing radiation.Chemical structures of daunorubicin, doxorubicin, and epidoxorubicin.
(a)
(b)
(c)The aim of this work was to assess the possibility of applying radiation sterilization to DOX, DAU, and EPI as active substances or conversion-induced impurities.


## 2. Materials and Methods

### 2.1. Samples

DOX, DAU, and EPI were synthesized at the Institute of Biotechnology and Antibiotics, Department of Modified Antibiotics, Warsaw, Poland. They were reddish powders, freely soluble in water and methanol. Sodium lauryl sulfate, phosphoric acid, and all other chemicals were obtained from Merck KGaA (Germany) and were of analytical or high-performance liquid chromatographic grade.


### 2.2. Irradiation

0.025 g samples of each substance were placed in 3 mL colorless glass vials that were closed with plastic stoppers. The samples in the vials were exposed to beta irradiation in a linear electron accelerator LAE 13/9 (9.96 MeV electron beam and 6.2μA current intensity) until they absorbed doses of 25, 50, 100, 200, and 400 kGy.


### 2.3. Electron Paramagnetic Resonance (EPR) Spectroscopy

Detection of free radicals and determination of their concentration were carried out using a Bruker ELEXSYS 500 spectrometer (X-band) at 297 K. EPR spectra were recorded as a first derivative of the absorption signal. The number of free radicals was calculated using the integration procedure described elsewhere [17].


### 2.4. UV-Vis Spectroscopy

Chemical changes in nonirradiated and irradiated samples were analyzed by using a UV-Vis Varian Carry 100 spectrophotometer. 2 mg of each sample was dissolved in 20.0 mL of methanol. 1.0 mL of the so-obtained solution was diluted to 10.0 mL with methanol. The final concentration of the solutions was 0.01 mg/mL. Absorption spectra of the so-prepared solutions were recorded in the wavelength range 190–900 nm.


### 2.5. IR Spectroscopy

IR spectra of nonirradiated and irradiated anthracyclines were taken with the use of a Thermo Scientific Nicolet iS10 spectrophotometer with the Omnic software. The infrared transmittance spectra of the crystalline samples were recorded after a time necessary to achieve plateaus in EPR study, in the frequency range from 400 to 7500 cm−1, at room temperature.


### 2.6. HPLC Analysis

An HPLC Waters Alliance e2695 system was used for chromatographic separation of the degradation products of nonirradiated and irradiated DOX, DAU and EPI samples. All the samples (1 mg/mL) were dissolved in the mobile phase. A Symmetry C18 (250 × 4.6 mm, 5μm) analytical column was employed as a stationary phase. The mobile phase consisted of solution A (acetonitrile) and solution B (2.88 g of sodium lauryl sulfate and 2.25 g of phosphoric acid(V) 85% in 1000 mL) (50 : 50, v : v). UV detection was performed at 254 nm. The flow rate was 1.0 mL/min. The injected volume was 5 μL.


### 2.7. Theoretical Analysis

All the calculations were made by using the Gaussian 03 package [18]. In order to interpret the experimental results of IR absorption scattering, quantum chemical calculations were performed based on a density functional theory (DFT) method with the B3LYP hybrid functional and 6-31G(d,p) basis set.


## 2.1. Samples

DOX, DAU, and EPI were synthesized at the Institute of Biotechnology and Antibiotics, Department of Modified Antibiotics, Warsaw, Poland. They were reddish powders, freely soluble in water and methanol. Sodium lauryl sulfate, phosphoric acid, and all other chemicals were obtained from Merck KGaA (Germany) and were of analytical or high-performance liquid chromatographic grade.


## 2.2. Irradiation

0.025 g samples of each substance were placed in 3 mL colorless glass vials that were closed with plastic stoppers. The samples in the vials were exposed to beta irradiation in a linear electron accelerator LAE 13/9 (9.96 MeV electron beam and 6.2μA current intensity) until they absorbed doses of 25, 50, 100, 200, and 400 kGy.


## 2.3. Electron Paramagnetic Resonance (EPR) Spectroscopy

Detection of free radicals and determination of their concentration were carried out using a Bruker ELEXSYS 500 spectrometer (X-band) at 297 K. EPR spectra were recorded as a first derivative of the absorption signal. The number of free radicals was calculated using the integration procedure described elsewhere [17].


## 2.4. UV-Vis Spectroscopy

Chemical changes in nonirradiated and irradiated samples were analyzed by using a UV-Vis Varian Carry 100 spectrophotometer. 2 mg of each sample was dissolved in 20.0 mL of methanol. 1.0 mL of the so-obtained solution was diluted to 10.0 mL with methanol. The final concentration of the solutions was 0.01 mg/mL. Absorption spectra of the so-prepared solutions were recorded in the wavelength range 190–900 nm.


## 2.5. IR Spectroscopy

IR spectra of nonirradiated and irradiated anthracyclines were taken with the use of a Thermo Scientific Nicolet iS10 spectrophotometer with the Omnic software. The infrared transmittance spectra of the crystalline samples were recorded after a time necessary to achieve plateaus in EPR study, in the frequency range from 400 to 7500 cm−1, at room temperature.


## 2.6. HPLC Analysis

An HPLC Waters Alliance e2695 system was used for chromatographic separation of the degradation products of nonirradiated and irradiated DOX, DAU and EPI samples. All the samples (1 mg/mL) were dissolved in the mobile phase. A Symmetry C18 (250 × 4.6 mm, 5μm) analytical column was employed as a stationary phase. The mobile phase consisted of solution A (acetonitrile) and solution B (2.88 g of sodium lauryl sulfate and 2.25 g of phosphoric acid(V) 85% in 1000 mL) (50 : 50, v : v). UV detection was performed at 254 nm. The flow rate was 1.0 mL/min. The injected volume was 5 μL.


## 2.7. Theoretical Analysis

All the calculations were made by using the Gaussian 03 package [18]. In order to interpret the experimental results of IR absorption scattering, quantum chemical calculations were performed based on a density functional theory (DFT) method with the B3LYP hybrid functional and 6-31G(d,p) basis set.


## 3. Results and Discussions

The first EPR analysis was performed 1 day after irradiation for samples exposed to a dose of 25 kGy. DOX, DAU, and EPI irradiated at 25 kGy contained about 3.94 × 1015 spins/g, 1.37 × 1015 spins/g, and 2.44 × 1015 spins/g, respectively. Exponential decay of unstable free radical was observed with the half-time of 4 days in DAU and DOX and 7 days in EPI after irradiation. The EPR signals of the sterilized anthracycline antibiotics were very weak. The plateaus of free radical concentrations versus time for DOX and DAU appeared after about 10 days, whereas for EPI after 20 days. EPR spectra after these periods consisted approximately of only stable free radicals.The analytical study was conducted during a period when only stable free radicals were detected by EPR. The impact of an irradiation dose size on the structure of DAU, DOX, and EPI was studied at 0, 25, 50, 100, 200, and 400 kGy without the presence  of unstable free radical in EPR spectra. By using UV-Vis spectroscopy the location and the intensity of the absorption maximum were determined, whereas IR spectroscopy was employed to establish the intensity, location, and type of characteristic vibrations. For the DAU, DOX, and EPI samples no significant changes in the location (~289 nm, ~233 nm, and 221 nm) or intensity of the absorption maximum were recorded (Figures2, 3, and 4). The spectra of their nonirradiated and irradiated samples did not show any essential differences in the value of absorbance. All samples exhibited two absorption maxima, at 233 nm and 251 nm. The IR spectra of DOX, DAU, and EPI were compared with the theoretical spectra based on the density functional theory. The main characteristic vibrations obtained from the IR spectra are collected in Table 1. The conformation between the calculated and experimental spectra is quite good. The most significant is the region between 700 and 1800 cm−1, where intense and characteristic bands related to intramolecular vibrations of the molecules are observed, including the deformation of rings as well as stretching of various C–C bonds (Figures 5, 6, and 7). Vibrational spectra of the nonirradiated and irradiated samples of the three samples are very similar. We did not observe any change in the position and shape of the bands. This suggests that the radiation sterilization does not influence the stability of the DOX, DAU, and EPI. Similar results were received by comparing SEM images of the nonirradiated and irradiated samples. Taking into account the biological activity of the most important impurities specified by Ph. Eur. [9], changes in the concentration of the main substances in the presence of those impurities were analyzed (Figures 8, 9, 10, 11, 12, and 13). By separating the compounds to be examined from the impurities, it was possible to assess changes in their content before and after irradiation at 25 kGy. It was found that exposure to such a dose of radiation did not produce any changes in the concentrations of the main substances or the impurities.Table 1
Main characteristic vibrational modes of daunorubicin (DAU), doxorubicin (DOX), and epidoxorubicin (EPI) observed in experimental and calculated spectra.
Calculation (cm−1)
Experimental (cm−1)
Band assignment
DAU
DOX
EPI
DAU
DOX
EPI
723
736
736
764
761
761
C–C–Cb in a ring + def. aglycone group + breathing tetrahydropyran ring in aminosugar group + NH2 w
752
754
749
795
795
795
NH2  w + C–C–C b in aminosugar group + C–H w at d ring and in aminosugar group
776
773
774
816
804
805
C–C–Cb in a ring + def. aglycone group + breathing tetrahydropyran ring in aminosugar group + CH2 r at d ring
931
930
935
940
939
939
C–O–Hb at c ring + C–H w in aminosugar group
959
957
951
955
949
949
C–C–Cb in d ring + NH2  w + CH w at d ring and aminosugar group
984
—
—
CH3 w in COCH3 group
1010
1008
1006
986
990
989
Breathing aglycone group + CH2  r + NH2  r + CH3  w in COCH3 + CH2  w in COCH2OH
1021
1029
1032
1008
1005
1005
Breathinga ring + def. aglycone group + C–O s at a ring + C–O–H b at c ring + NH2  w + CH3  w in COCH3 group
1079
1078
1065
1070
1072
1072
C–Os between tetrahydropyran ring and O–H group in aminosugar group
1105
1104
1104
1085
1089
1089
C–Os in metoxy group at a ring + C–C–C b in a ring + CH2  r
1123
1123
1122
1109
1114
1114
C–Os in tetrahydropyran ring + C–O s in glycosidic bond + C–C s in d ring + C–H w in CH3 in aminosugar group
1137
1139
1136
1109
1114
1114
C–Cs in d ring and in tetrahydropyran ring in aminosugar group + C–H b at d ring and in tetrahydropyran ring in aminosugar group + CH3  w in COCH3 group + C–O s in COCH2OH group
1157
1157
1156
1153
1143
1143
C–Os in glycosidic bond + C–O s between tetrahydropyran ring and O–H group in aminosugar group + C–H r in CH3 in aminosugar group + def. dring
1206
1205
1200
1194
1201
1201
C–Os in glycosidic bond + C–C s I d ring + C–H b at d ring and aminosugar group
1226
1225
1221
1205
1211
1211
CH2  t at d ring + breathing a ring + C–O–H b at c ring
—
1239
1239
1235
1235
C–O–Hb in COCH2OH group
1278
1275
1275
1262
1263
1263
C–O–Hb at c ring + C–C s in aglycone group + CH2  t in COCH2OH group
1291
1290
1293
1289
1284
1285
C–O–Hb at c ring + breathing a and c ring
1317
1317
1318
1289
1284
1285
Breathingc ring + C–O s at c ring + C–O s at a ring + CH2 w at a ring +C–H w at d ring
1329
1329
1330
1317
1318
1318
C–Os at a ring + breathing a and c ring
1367
1367
1366
1374
1374
1374
C–C–Cb in d ring + C–C s in a and b ring + C–O s at c ring + C–O– b at c ring
1404
—
—
CH3  sc in COCH3 group
1425
1427
1423
1404
1413
1413
O–C–Hb + N–C–H b
1476
1476
1478
1474
1471
1472
C–Os at c ring + def. c ring + CH3  umbrella mode at a ring + C–O–H b at c ring
1502
1502
1505
1506
1507
1507
C–O–Hb at c ring + CH3  sc at a ring + C–C s in aglycone group
1524
1524
1527
1525
1524
C–O–Hb at c ring + CH3  sc at a ring + C–C s in aglycone group
1614
1615
1615
1576
1582
1581
C–Cs in c ring + C–O–H b at c ring + C=O s at b ring + C–C s in a ring
1640
1640
1642
1576
1582
1581
C–Cs in a ring + C–O–H b at c ring + CH3  w at a ring
1661
1663
1663
1617
1616
1616
NH2  sc
1688
1688
1688
1617
1616
1616
C=Os at b ring + C–O–H b at c ring
1744
1744
1745
1707
1717
1717
C=Os at b ring
1791
1808
1807
1716
1730
1730
C=Os at d ring
2967–3237
2844–3108
C–Hs
3009
3010
2960
2878
2896
2896
O–Hs at c ring
3488
3488
3485
N–Hs symmetric
3566
3566
3571
N–Hs antisymmetric
3630
3634
3639
3161
3326
3329
O–Hs
3791
3788
3785
3527
3527
O–Hs
3809
3801
—
O–Hs
—
3831
3831
3545
3545
O–Hs in COCH2OH group
Vibrational modes—s: stretching, b: bending, w: wagging, sc: scissoring, r: rocking, and t: twisting.Figure 2
UV spectra of unirradiated and gamma irradiated daunorubicin.Figure 3
UV spectra of unirradiated and gamma irradiated doxorubicin.Figure 4
UV spectra of unirradiated and gamma irradiated epidoxorubicin.Figure 5
FT-IR spectra of unirradiated and gamma irradiated daunorubicin.Figure 6
FT-IR spectra of unirradiated and gamma irradiated doxorubicin.Figure 7
FT-IR spectra of unirradiated and gamma irradiated epidoxorubicin.Figure 8
HPLC profile of unirradiated daunorubicin.Figure 9
HPLC profile of irradiated daunorubicin (25 kGy).Figure 10
HPLC profile of unirradiated doxorubicin.Figure 11
HPLC profile of irradiated doxorubicin (25 kGy).Figure 12
HPLC profile of unirradiated epidoxorubicin.Figure 13
HPLC profile of irradiated epidoxorubicin (25 kGy).Slight alterations were registered when the samples of DAU, DOX, and EPI were exposed to greater doses of radiation. Under such conditions, EPI demonstrated the greatest content change, and the presence of unstable free radicals was noted for the longest period of time. It was also proved, by observing the mass balance, that the main substances did not convert into unknown impurities. Similar studies of some tetracycline analogs showed that the aglycon was stable when irradiated at 25 kGy and that changes occurred when greater radiation doses were applied [19]. It may therefore be proposed that not only a modification of the aglycon structure but also its ability to bind with a sugar moiety of specific stereoisomerism are the factors that stabilize the structures of analogs of anthracycline antibiotics.


## 4. Conclusions

The current study of the impact of radiation sterilization on the stability of DAU, DOX, and EPI demonstrates that this kind of sterilization may be an alternative to filtration recommended for sterilizing analogs of anthracycline antibiotics. The effect of radiation sterilization on the stability of DAU, DOX, and EPI depends on the structure of a particular compound. With regard to those analogs, it is important to assay the postirradiation content of the main substance in the presence of all possible related substances in order to determine whether other degradation products or postirradiation conversion occur.



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*Source: 258758-2013-11-03.xml*