# 1H and 13C NMR Assignments of Cytotoxic 3S-1,2,3,4-Tetrahydro-β-carboline-3-carboxylic Acid from the Leaves of Cichorium endivia

Authors: Fu-Xin Wang; An-Jun Deng; Jin-Feng Wei; Hai-Lin Qin; Ai-Ping Wang
Journal: Journal of Analytical Methods in Chemistry
 (2012)

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## Abstract

An amino acid, 3S-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid, was isolated for the first time from the leaves of Cichorium endivia. The complete assignment of its 1H and 13C NMR spectroscopic data was carried out also for the first time based on extensive 1D and 2D NMR experiments. Cytotoxicity of this isolated compound against HCT-8 and HepG2 human cancer cell lines was evaluated for the first time, with moderate activities being found.


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## Body

## 1. Introduction

Cichorium endivia L. is a popular vegetable from the family of Compositae and is widely cultivated and consumed all over the world. Its popularity is also attributed to the healthy properties mainly due to supply of antioxidant activity [1, 2]. However, phytochemical investigation on this plant is very rare up to now, to the best of our knowledge, only a few papers had reported a few compounds, including five ones by our group [3]. The ongoing research aims at confirming the bioactive compounds from this popular vegetable, and a prevailing and known amino acid, 3S-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid (1), was isolated for the first time. By way of the literature survey, it can be learned that the complete assignment of the NMR data of 1 was very deficient up to now, with no practical conducting being obtained due to the poor solubility of 1 in most prevalent solvents and causing the citation of literatures an obvious state of chaos [4, 5]. In this paper, we describe the complete assignment of the 1H and 13C NMR spectroscopic data of 1 based on the determining of optimized solvent and extensive 1D and 2D NMR experiments. An investigation focusing on the cytotoxicity of compound 1 against HCT-8 and HepG2 human cancer cell lines showed that 1 inhibits the cells growth by a moderate reduction in viability of subjects.


## 2. Results and Discussion

Compound1 was isolated as an amorphous pale-yellow powder (MeOH/H2O). Its positive-ion ESI-MS spectrum showed the quasimolecular ion peaks at m/z 217.1 [M+H]+and 239.1 [M+Na]+, and its molecular formula was established to be C12H12N2O2 by the quasimolecular ion peak in the positive mode HRESI-MS experiment at m/z 217.0967 [M+H]+. The IR spectrum showed strong absorptions at υmax 3284, 3019, 1642, 1598, 1452, 1409, and 740 cm−1, indicating the presence of 1,2-disubstitued benzene moiety and labile hydrogen. It was preliminarily identified as 3S-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid by comparison of the 1H NMR spectroscopic data obtained in DMSO-d6 with the literature values [4, 5], but some obvious errors or inconsistency were evident, including the coupling constants and data ownership (Table 1). Whereas the obtainment of 13C NMR spectrum in the NMR solvent of DMSO-d6 was very difficult due to the above-mentioned poor solubility, compound 1 was then recorded the 1D and 2D NMR spectra within D2O + drops of F3CCOOD, which proved to be a good solvent for 1. The 1H NMR spectroscopic data also clearly revealed the existence of 1,2-disubstitued benzene moiety, with four diagnostic signals from an aromatic ABCD spin system resonating at δH 7.13 (1H, t, J=7.6 Hz, H-6), 7.22 (1H, t, J=8.0 Hz, H-7), 7.43 (1H, d, J=8.0 Hz, H-8), and 7.53 (1H, d, J=8.0 Hz, H-5), which correlated to the aromatic carbon signals at δC 120.0 (C-6), 122.9 (C-7), 111.9 (C-8), and 118.2 (C-5), respectively, in the HSQC spectrum. In addition, five well-resolved and characteristic signals at δH 4.38 (1H, d, J=15.6 Hz, H-1a), 4.54 (1H, d, J=15.6 Hz, H-1b), 4.27 (1H, dd, J=10.4, 5.6 Hz, H-3), 3.13 (1H, dd, J=10.8, 16.4 Hz, H-4a), and 3.38 (1H, dd, J=5.6, 16.4 Hz, H-4b) were also examined in the 1H NMR spectrum, which were, conveniently according to their coupling constants and with the aid of 1H, 1H-COSY spectrum, assigned to one AB spin system from an isolated methylene group and one ABX system from one methylene and one methine correlated together by sp3 hybridized bond. These three functional groups were obviously deshielded and were correlated to their corresponding carbon signals at δC 40.5 (C-1), 55.0 (C-3), and 21.7 (C-4), respectively, in the HSQC spectrum. Simply, these three groups were arranged to either sp2 hybridized carbons or nitrogen atoms when considering the 1H and 13C NMR chemical shifts and examining the 13C and DEPT NMR data which exhibited twelve carbon signals with two aliphatic methylenes, one aliphatic methine, four sp2 hybridized methines, and five sp2 hybridized quaternary carbons being categorized (Table 1). The above NMR data were compatible with a benzene moiety, a tetrasubstituted ethylene, and a carbonyl except for the above-mentioned three aliphatic carbons. The long range 1H,13C-correlations from δH 7.53 to δC 104.9, 125.6, 136.7, and 122.9, from δH 7.43 to δC 122.9, 120.0, and 125.6, from δH 7.22 to δC 111.9, 136.7, and 118.2, from δH 7.13 to δC 118.2, 125.6, 122.9, and 111.9, from δH 4.38 and 4.54 to δC 125.4, 104.9, and 55.0, from δH 3.13 and 3.20 to δC 104.9, 55.0, 171.3, and 125.6/125.4, and from δH 4.27 to δC 171.3, 40.5, 21.7, and 104.9 established the constitutional formula of 1as indicated by Figure 1. The complete assignment of the NMR data is listed in Table 1.Table 1
1H and 13C NMR spectroscopic data for compound 1.
Number
δ
H
ab
δ
C
ac
δ
H
de
δ
H
d [4]
δ
H
d [5]
δ
C
d [5]
1a
4.38 d (15.6)
40.5 t
4.15 d  (15.3)
—
—
40.3
1b
4.54 d  (15.6)
4.23 d  (15.9)
4.22 d  (4.8)
—
3
4.27 dd  (10.4, 5.6)
55.0 d
3.60 m
3.14
—
55.3
4a
3.13 dd  (10.8, 16.4)
21.7 t
2.81 dd-like
2.83 ddd  (10.5, 5.0,  2.4)
—
18.0
4b
3.38 dd  (5.6, 16.4)
3.13 br d-like
3.69 dd  (10.5, 5.0)
—
4a′
104.9 s
104.3
4b′
125.6f s
128.5
5
7.53 d  (8.0)
118.2 d
7.32 d  (7.5)
7.33 d  (8.0)
7.38 d  (8.2)
118.5
6
7.13 t  (7.6)
120.0 d
7.06 t  (7.5)
7.08 t  (8.0)
7.06 t  (8.2)
117.5
7
7.22 t  (8.0)
122.9 d
6.97 t  (7.8)
6.99 t  (7.5)
6.96 t  (8.2)
121.1
8
7.43 d  (8.0)
111.9 d
7.43 d  (7.5)
7.44 d  (7.5)
7.44 d  (8.2)
111.8
8a
136.7 s
136.1
9a
125.4f s
—
COOH
171.3 s
10.91 s
10.93 s
—
165.6
9-NH
—
10.66 s
aD2O + drops of F3CCOOD; b400 MHz. c100 MHz. din DMSO-d6; e300 MHz. fAssignments may be interchanged.Figure 1
The structure of compound1 and the key HMBC (→ : H→C) correlations.On evaluation of compound1 for its cytotoxic effects on two human cancer cell lines, cell growth was measured using a sulforhodamine B (SRB) assay. Results of means of three replicates are expressed as the percentage of viability compared to negative control. Compound 1 exhibited moderate cytotoxicities against HCT-8 and HepG2 cell-lines in the evaluation, with viability of HepG2 and HCT-8 cells being 80.42% and 80.22% after treatment for 48 hours, and 76.14% and 71.48% after 72 hours, respectively, when using a concentration of 140 μg/mL. The viabilities at other time points were relatively lower.


## 3. Experimental

### 3.1. General Experimental Procedures

IR spectra were obtained on a Nicolet 5700 spectrometer. 1D and 2D NMR spectra were recorded on a Mercury-400 or a MERCURY-300 NMR spectrometer. Chemical shifts (δ) were given in ppm using tetramethylsilane (TMS) as internal standard (δ 0.00). ESI-MS and HRESI-MS were measured on an Agilent 1100 series LC-MSD-Trap-SL spectrometer. RP-18 (YMC-GEL, ODS-A, 12 nm, S-50 mm; YMC Co., Kyoto, Japan) were used for column chromatography. Solvents were of analytical grade and were purchased from Beijing Chemical Company (Beijing, China).


### 3.2. Plant Material

Cichorium endivia was purchased from Beijing Xinfadi agricultural products wholesale market on July 2009 and authenticated by Associate Professor Ma Lin (Institute of Material Medica, Chinese Academy of Medical Sciences and Peking Union Medical College). A voucher specimen was deposited in New Drug Safety Evaluation Center, Institute of Materia Medica, Peking Union Medical College & Chinese Academy of Medical Sciences, China.


### 3.3. Extraction and Isolation

The air-dried and pulverizedC. endivia (5.8 kg) was extracted three times under reflux conditions with 95% EtOH (80 L, 2 h; 70 L, 1 h; 68 L, 1 h). The combined EtOH extracts were evaporated in vacuum to yield a dark-green residue (1233 g, crude EtOH extract), which was suspended in 80% aq. EtOH. The resulting suspension was extracted with petroleum ether (60–90°C, 2 L, 1.5 L, 1.5 L, and 1.5 L). Evaporation of the aq. layers in vacuum yielded also a dark-green residue (940 g, 80% EtOH extract). The residue was redissolved in water and subsequently partitioned with EtOAc in separatory funnel exhaustively. The rest of water soluble fraction was loaded on a column filled with Daion HP-20 and eluted with H2O, 60% EtOH, and 95% EtOH, respectively. The eluates of 60% EtOH were evaporated in vacuum which yielded a black residue (40 g).The 60% EtOH fraction was redissolved in solvent ofn-BuOH and washed with aq. 5% NaHCO3 then H2O, respectively. Evaporation of n-BuOH under reduced pressure gave 5.5 g of brown-green residue, which was submitted to an ODS-A column eluted with MeOH-H2O of decreasing polarity (40%–100%) to yield compound 1 (108 mg) as pale-yellow powder.


#### 3.3.1.  3S-1,2,3,4-Tetrahydro-β-carboline-3-carboxylic Acid (1)

Pale-yellow powder. IR (KBr)υmax (cm−1): 3284, 3019, 2849, 1642, 1598, 1452, 1409, 1271, 1221, and 740; 1H and13C NMR spectroscopic data are listed in Table 1. ESI-MS (positive mode) m/z: 217.1 [M+H]+and 239.1 [M+Na]+; HRESI-MS (positive mode) m/z: 217.0967 [M+H]+(calcd for C12H12N2O2, 217.0972).


### 3.4. Cytotoxicity Assay

HCT-8 and HepG2 cells were cultivated in RPMI1640 medium containing 0.22% sodium bicarbonate, 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100μg/mL streptomycin. The cells were incubated in 5% CO2-air at 37°C. Compound 1 was dissolved in phosphate-buffered saline (PBS) at a concentration of 1.4 mg/mL and was diluted to the required concentration with RPMI1640 medium immediately before use.The cell viability was measured by using sulforhodamine B (SRB) assay. Briefly, the cells were seeded in 96-well plates (1×104 cells/well) and routinely cultured for 24 h. Compound 1 was added to in-serial concentrations (from 14 μg/mL to 140 μg/mL), while PBS was added alone to control wells as a negative control, and incubation was continued for an additional 48 h. SRB (1 mg/mL) was added to each well after the plates were fixed using TCA (0.4% m/v). After 20 minutes of incubation, each well was washed by acid (1% v/v) three times. Then wells were added into Tris (100 mmol/L), respectively. The absorbance of each well was recorded on a microplate spectrophotometer at 515 nm.


## 3.1. General Experimental Procedures

IR spectra were obtained on a Nicolet 5700 spectrometer. 1D and 2D NMR spectra were recorded on a Mercury-400 or a MERCURY-300 NMR spectrometer. Chemical shifts (δ) were given in ppm using tetramethylsilane (TMS) as internal standard (δ 0.00). ESI-MS and HRESI-MS were measured on an Agilent 1100 series LC-MSD-Trap-SL spectrometer. RP-18 (YMC-GEL, ODS-A, 12 nm, S-50 mm; YMC Co., Kyoto, Japan) were used for column chromatography. Solvents were of analytical grade and were purchased from Beijing Chemical Company (Beijing, China).


## 3.2. Plant Material

Cichorium endivia was purchased from Beijing Xinfadi agricultural products wholesale market on July 2009 and authenticated by Associate Professor Ma Lin (Institute of Material Medica, Chinese Academy of Medical Sciences and Peking Union Medical College). A voucher specimen was deposited in New Drug Safety Evaluation Center, Institute of Materia Medica, Peking Union Medical College & Chinese Academy of Medical Sciences, China.


## 3.3. Extraction and Isolation

The air-dried and pulverizedC. endivia (5.8 kg) was extracted three times under reflux conditions with 95% EtOH (80 L, 2 h; 70 L, 1 h; 68 L, 1 h). The combined EtOH extracts were evaporated in vacuum to yield a dark-green residue (1233 g, crude EtOH extract), which was suspended in 80% aq. EtOH. The resulting suspension was extracted with petroleum ether (60–90°C, 2 L, 1.5 L, 1.5 L, and 1.5 L). Evaporation of the aq. layers in vacuum yielded also a dark-green residue (940 g, 80% EtOH extract). The residue was redissolved in water and subsequently partitioned with EtOAc in separatory funnel exhaustively. The rest of water soluble fraction was loaded on a column filled with Daion HP-20 and eluted with H2O, 60% EtOH, and 95% EtOH, respectively. The eluates of 60% EtOH were evaporated in vacuum which yielded a black residue (40 g).The 60% EtOH fraction was redissolved in solvent ofn-BuOH and washed with aq. 5% NaHCO3 then H2O, respectively. Evaporation of n-BuOH under reduced pressure gave 5.5 g of brown-green residue, which was submitted to an ODS-A column eluted with MeOH-H2O of decreasing polarity (40%–100%) to yield compound 1 (108 mg) as pale-yellow powder.


### 3.3.1.  3S-1,2,3,4-Tetrahydro-β-carboline-3-carboxylic Acid (1)

Pale-yellow powder. IR (KBr)υmax (cm−1): 3284, 3019, 2849, 1642, 1598, 1452, 1409, 1271, 1221, and 740; 1H and13C NMR spectroscopic data are listed in Table 1. ESI-MS (positive mode) m/z: 217.1 [M+H]+and 239.1 [M+Na]+; HRESI-MS (positive mode) m/z: 217.0967 [M+H]+(calcd for C12H12N2O2, 217.0972).


## 3.3.1.  3S-1,2,3,4-Tetrahydro-β-carboline-3-carboxylic Acid (1)

Pale-yellow powder. IR (KBr)υmax (cm−1): 3284, 3019, 2849, 1642, 1598, 1452, 1409, 1271, 1221, and 740; 1H and13C NMR spectroscopic data are listed in Table 1. ESI-MS (positive mode) m/z: 217.1 [M+H]+and 239.1 [M+Na]+; HRESI-MS (positive mode) m/z: 217.0967 [M+H]+(calcd for C12H12N2O2, 217.0972).


## 3.4. Cytotoxicity Assay

HCT-8 and HepG2 cells were cultivated in RPMI1640 medium containing 0.22% sodium bicarbonate, 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100μg/mL streptomycin. The cells were incubated in 5% CO2-air at 37°C. Compound 1 was dissolved in phosphate-buffered saline (PBS) at a concentration of 1.4 mg/mL and was diluted to the required concentration with RPMI1640 medium immediately before use.The cell viability was measured by using sulforhodamine B (SRB) assay. Briefly, the cells were seeded in 96-well plates (1×104 cells/well) and routinely cultured for 24 h. Compound 1 was added to in-serial concentrations (from 14 μg/mL to 140 μg/mL), while PBS was added alone to control wells as a negative control, and incubation was continued for an additional 48 h. SRB (1 mg/mL) was added to each well after the plates were fixed using TCA (0.4% m/v). After 20 minutes of incubation, each well was washed by acid (1% v/v) three times. Then wells were added into Tris (100 mmol/L), respectively. The absorbance of each well was recorded on a microplate spectrophotometer at 515 nm.



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*Source: 254391-2012-12-16.xml*