# Analysis of Anti-Infective Treatment of 9 Neonates withRaoultella ornithinolytica Sepsis

Authors: Jing Li; Yan Zhuang; Dingliang Xiao; Haixia Zhang; Fangmei Luo; Jinhua He
Journal: Evidence-Based Complementary and Alternative Medicine
 (2022)

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## Abstract

Raoul ornithine-releasing bacteria widely exist in water, plants, and soil, and colonize the digestive tract and upper respiratory tract of the human body. They are aerobic, unpowered, and capsular opportunistic pathogens. The infectivity of this bacterium is still uncertain, but the possibility of nosocomial infection has been mentioned in the literature. Studies have pointed out that the bacterium should be diagnosed in time and sensitive antibiotics should be used early. Once complicated with sepsis, it can cause multiple organ failure with a poor prognosis. In this study, we retrospectively analyzed the clinical data of nine cases of neonatal L. ornithine septicemia, to explore the clinical characteristics of neonatal L. ornithine septicemia and anti-infection therapy.


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## Body

## 1. Introduction

In 1989, Sękowska [1] first proposed that Raoule ornithinolytica was an aerobic, amotile, and encapsulated opportunistic pathogen. Raoulella ornitholytica was first classified as Klebsiella in the 1980s, but was reclassified as Klebsiella in 2001 because 16SrRNA and rpoB gene analysis showed that it was not consistent with Klebsiella [2]. In 2009, Morais et al. [3] reported cases of human infection with Raoulia ornitholytica. In recent years, the infection of L. ornithine-releasing bacteria is mostly reported in adults, the infection cases in children are less reported, and the infection cases in neonates are even less reported. [4–7]. In order to explore the clinical features and anti-infective treatment plan of neonatal Raul Ornithinolytica sepsis, 9 cases of neonatal Raul Ornithinolytica sepsis in our hospital were retrospectively analyzed.


## 2. Objects and Methods

### 2.1. Research Objects

The subjects of this study were children diagnosed with Raoultia ornithine septicemia in the department of neonatology of our hospital from July 2020 to December 2021.The diagnostic criteria were positive blood bacterial culture, clinical symptoms and signs of bacterial infection, and abnormal laboratory test results (blood routine, C-reactive protein (CRP), procalcitonin (PCT), interleukin-6 (IL-6) and other infection indicators) [8].


### 2.2. Research Methods

In this study, a retrospective analysis was performed. Electronic medical records were consulted to record children’s age, gender, maternal and pregnancy status, clinical manifestations, medication history, hospitalization time, hospitalization diagnosis, previous diseases, laboratory tests, auxiliary examinations, treatment, medication status, statistical analysis of the data, and prognosis.


## 2.1. Research Objects

The subjects of this study were children diagnosed with Raoultia ornithine septicemia in the department of neonatology of our hospital from July 2020 to December 2021.The diagnostic criteria were positive blood bacterial culture, clinical symptoms and signs of bacterial infection, and abnormal laboratory test results (blood routine, C-reactive protein (CRP), procalcitonin (PCT), interleukin-6 (IL-6) and other infection indicators) [8].


## 2.2. Research Methods

In this study, a retrospective analysis was performed. Electronic medical records were consulted to record children’s age, gender, maternal and pregnancy status, clinical manifestations, medication history, hospitalization time, hospitalization diagnosis, previous diseases, laboratory tests, auxiliary examinations, treatment, medication status, statistical analysis of the data, and prognosis.


## 3. Results

### 3.1. Basic Information

From July 2020 to December 2021, a total of 9 cases of Raoulia ornithinolyticum sepsis were diagnosed in the department of neonatology of our hospital, from 3 neonatal wards, including 2 cases in the NICU ward, 3 cases in the surgical ward, and 4 cases in the general ward. There were 7 boys and 2 girls; only 1 was a full-term neonate (37 weeks of gestation), and the remaining 8 were premature infants, the basic situation of children is shown in Table1.Table 1
Basic information of children with Raoulia ornithine solution sepsis.
CaseGenderGestational week (W)Cause of premature birthCesarean sectionBirth weight (kg)History of suffocationWhether it is a twin or multiple birthIs it a test tube babyAge at admission (d)Maternal pregnancy history1Male30 + 1Labor initiationYes1.4YesNoNo80G2P2, pregnancy-induced hypertension2Female27Onset of labor, massive bleeding from placenta previa, and premature rupture of membranesYes0.9YesNoNo68G5P2, hypothyroidism, GDM3Male32 + 6Premature rupture of membranesNo2.7NoNoNo3G2P24Female28 + 2Premature rupture of membranesNo0.9NoTwinsYes84G1P25Male35 + 2Placental abruptionYes2.4NoNoYes<1 (2 h)G5P16Male36 + 1Labor initiationNo3.3NoNoNo3G2P27Male37-No2.4UnknownNoNo15G2P28Male28 + 6Maternal cervical insufficiency and premature rupture of membranesYes1.45NoTripletsNo31G5P49Male33 + 3Onset of labor and premature rupture of membranesNo2.1YesNoNo<1 (6 h)G6P2Respiratory patterns before infection occurred in 9 patients: case 6 was ventilated by using a noninvasive ventilator, the case 4 was given high-flow oxygen, in case 8 was given oxygen by nasal cannula, and the remaining cases did not need oxygen therapy. The use of antibiotics before infection occurred in 9 children: 3 cases did not use antibiotics; the remaining 6 cases all using broad-spectrum antibiotics, including cefoperazone-sulbactam, meropenem, vancomycin, imipenem, cilastatin sodium, and linezolid from birth to the time of the infection and other antibiotics. The other 9 children all required intravenous nutrition; cases 1 and 6 had PICC intubation.


### 3.2. Blood Routine and Infection Index Monitoring

All the 9 patients had at least one or more abnormal indicators, and all the children had reduced platelets. In case 7, IL-6 was significantly elevated under normal conditions of other indicators. With effective anti-infective treatment, the levels of CRP and PCT in 7 children returned to normal, and the platelet count also gradually returned to normal. However, in case 4 and case 7, the inflammatory indicators did not decrease significantly or were at a continuous high value, and the platelet count gradually decreased or did not return to normal, as shown in Table2.Table 2
Changes of blood routine and inflammatory indexes in children with Raoultia.
CasesDays of infectionMonitoring timeWBC (×109•L−1)PLT (×109•L−1)Hb (g•L−1)NLCRP (g•L−1)PCT (μg•L−1)IL-6 (ng•L−1)1The same day9.1923.0681070.7370.23513.842.11>5000Day 29.208.22131110.5240.43394.1653.6—Day 39.2117.142730.6680.25397.1615.6826.66Day 49.227.6527950.3290.55431.897.5332.23Day 69.2416.2880950.590.28618.111.4922.48Day 99.2717.661061390.6790.2764.460.4410.53Day 119.3015.521481020.5190.40535.590.3613.172The same day8.238.8311990.610.25249.8519.82266Day 28.245.84720.6191.66245.6136.283356Day 38.256.226890.6920.236213.35——Day 48.266.121020.7360.22117.928.1771.34Day 68.2811.5614870.5870.22847.593.121.29Day 98.3118.17281250.6760.2075.890.415.63Day 169.78.15150910.510.28810.270.17.883The same day9.1412.463361250.780.71.9636.33Day 39.162.831121380.3530.6250.926.87>5000Day 49.1730.57171090.880.061103.43>100>5000Day 69.195628800.8640.0582.343.579.65Day 89.2147.38111460.6770.13215.7——Day 129.2515.97801130.6180.2366.460.343.064The same day9.23.872001270.5160.3986.431.75>5000Day 29.38.62151090.6550.21108.1935.421232Day 49.58.844650.7350.243170.7320.6730815The same day8.1515.32971130.8020.136106.7847.133948Day 28.1619.09811100.6570.19850.3212.51—Day 48.186.72146950.3910.32714.4——Day 98.237.68544990.2430.5471.760.14—Day 148.306.39390780.230.4980.94——Day 209.57.48315970.2850.5081.7——6The same day8.319.05303980.8680.06449.412.001191Day 39.25223850.4760.43270.261.81164.6Day 79.65.418740.6940.277116.4616.45311.9Day 89.713.9220950.5590.32881.446.232.52Day 109.911.921691030.5030.43113.30.61<1.5Day 149.136.77344970.3750.5161.80.11—7The same day9.78.58508920.5070.3941.890.223122Day 29.84.09208830.6950.262109.768.4542.2Day 39.93.84113870.4350.484163.66——Day 49.105.6932750.3820.476178.988.39Day 59.114.45191190.2570.639146.18——Day 69.129.2969970.3920.463100.8234.8523.16Day 79.1314.27820.7510.182101.6243.6140.7Day 89.1425.5472930.6850.20191.31——8The same day8.346.56135980.9590.01437.5810.57>5000Day 28.448.9922850.8250.101164.612.14842.5Day 38.535.19301450.7970.12184.664.4341.49Day 68.810.17891240.5980.24821.220.3825.93Day 18.147.322371010.4210.36620.111.959The same day9.166.782091260.8090.18952.84.53—Day 29.1724.36381040.6670.23615.9246.9634.04Day 39.1826.741001060.6130.28379.0525.183.42Day 59.2015.11230990.4180.48614.972<1.5Day 89.2311.22631930.3010.5851.590.223.54Day 139.2811.21578770.5820.2972.70.113.89


### 3.3. Clinical Features

Among the 9 children with Raoultia ornitholyticum septicemia, 7 had intestinal diseases, including 2 intestinal malformations and 5 neonatal necrotizing enterocolitis (NEC), of which 4 were had a history of intestinal surgery before Raoulia acidic infection. Among the other 9 cases, 2 cases had PICC catheter-related bloodstream infection, 2 cases had abnormal cerebrospinal fluid results and intracranial infection was considered, and 4 cases had different degrees of infection complications. In terms of clinical manifestations, 8 children had fever, of which 7 children showed repeated fever, and the remaining children showed changes in breathing, blood oxygen, and reaction. The length of hospital stay at the time of infection varies from 4 to 50 days, as shown in Table3 . In addition, after the occurrence of sepsis, 4 patients required invasive tracheal intubation for respiratory support, and 4 patients required oxygen therapy.Table 3
Clinical characteristics of 9 neonates with Raoulia.
CasesPrimary diseaseSurgery situationNumber of days of surgery at the time of infectionDays in the hospital at the time of infectionBacterial identificationClinical manifestationsWhether combined with intracranial infectionInfection complications1Ileal scarring strictures after NEC and premature infantsAdhesion bowel release, stricture bowel, and ileocecal resection819Blood and PICC catheter tipFever, shortness of breath, and nasal flaringNoLiver damage2NEC、BPD、 premature babyNo—7BloodDecreased blood oxygen and heart rateNoDIC3Congenital jejunal atresia (diaphragmatic type), enteric nerve dysplasia, and premature infantsEnteroplasty26BloodFever, poor response, frequent apnea, and decreased blood oxygenNoKidney damage4Premature infants and chronic lung diseaseNo—50BloodFever, poor mental response, visible markings all over the body, and vomiting of white mucus-like fluidYesSeptic shock, multiple organ dysfunction: Liver, kidney, myocardial damage, abnormal coagulation function, and ascites5Necrotizing enterocolitis in premature infants and neonatesNo—7BloodFeverYesNo6Congenital malrotation with midgut volvulus and intestinal necrosis and left testicular torsion with necrosisNecrotic bowel resection3132Blood and PICC lateral bloodFever and slightly poor mental responseNoNo7Neonatal necrotizing enterocolitisNo—4BloodRepeated fever for 9 daysNoNo8Neonatal necrotizing enterocolitis, premature infants, and BPDJejunostomy1819BloodFever and occasional transient oxygen desaturationNoNo9Aspiration pneumonia and premature infantsNo—27BloodFever with shallow and irregular breathingNoNo


### 3.4. Drug Susceptibility Results

14 strains were cocultured from 9 neonates with Raoultia ornitholyticum septicemia, 11 strains were carbapenem-resistant strains, of which 6 strains were resistant to levofloxacin, tigecycline, amikacin, and Compound sulfamethoxazole. 5 strains were only sensitive to tigecycline. The remaining 3 strains were sensitive strains, as shown in Table4.Table 4
Statistics of drug susceptibility results of 9 cases of neonatal Raoulia ornithinolytica sepsis.
CasesSpecimenPositive report time (h)Amoxicillin-clavulanate potassiumCefepimeCefoperazone-sulbactamCefoxitinCeftazidimeCeftriaxoneImipenemLevofloxacinPiperacillin-tazobactam sodiumTigecyclineAmikacinCotrimoxazole1Blood9RRRRRRRSRSSSBlood15RRRRRRRSRSSSPICC catheter tip—RRRRRRRSRSSSBlood17RRRRRRRSRSSS2Blood16RRRRRRRSRSSS3Blood16RRRRRRRSRSSS4Blood12RRRRRRRRRSRR5Blood17RRRRRRRRRSRRBlood53RRRRRRRRRSRR6Blood20RRRRRRRRRSRRPICC tube side blood12RRRRRRRRRSRR7Blood13SSSSSSSSSSSS8Blood15SSSSSSSSSSSS9Blood13SSSSSSSSSSSS


### 3.5. Anti-Infective Treatment and Outcome

Cases 1–6 are children with carbapenem-resistant bacteria infection. Among them, cases 1–3 were selected according to drug susceptibility to two sensitive drugs: the infection was effectively controlled by levofloxacin combined with amikacin treatment. In cases 5 and 6, the infection was also effectively controlled by removing the PICC catheter, increasing the dose of carbapenem, and prolonging the infusion time. Case 4 died of infection. Cases 7–9 were infected with susceptible strains, and cases 8 and 9 were effectively controlled by selecting sensitive drugs. In case 7, although a sensitive drug was selected for treatment, the effect was not good, and the parents of the child requested to be discharged from the hospital, as shown in Table5.Table 5
Anti-infective treatment and outcome of 9 cases of Raoulia ornitholyticum sepsis.
CasesUse anti-infective drugs and time of use (d)Other drug treatmentsTreatment outcome1Meropenem (1d), levofloxacin + amikacin (14 d)Removal of PICC tube, immunoglobulin, platelets, furosemide, packed red blood cells, frozen plasma, and human albuminCure2Meropenem (1d), levofloxacin + amikacin (14 d)Frozen plasma, furosemide, platelets, immunoglobulin, methylphenidate, packed red blood cells, and human serum albuminCure3Meropenem (2d), levofloxacin + amikacin + (14 d)Furosemide, human albumin, dopamine, platelets, and packed red blood cellsCure4Meropenem + amikacin (3 d)Immune globulin, platelets, packed red blood cells, frozen plasma, and furosemideDeath5Meropenem (40 mg/kg/time Q8H extended infusion time to 3 h, 21 d)ImmunoglobulinCure6Imipenem cilastatin sodium (25 mg/kg/time Q6H prolonged infusion time 2 h, 14 d)Remove the PICC tubeCure7Meropenem (40 mg/kg/time Q8H 8 d)Human immunoglobulin, furosemide, leukocyte-depleted suspended red blood cells, and plateletsUnknown (request for discharge)8Meropenem (14 d)NoCure9Meropenem + amikacin (14 d)NoCure


## 3.1. Basic Information

From July 2020 to December 2021, a total of 9 cases of Raoulia ornithinolyticum sepsis were diagnosed in the department of neonatology of our hospital, from 3 neonatal wards, including 2 cases in the NICU ward, 3 cases in the surgical ward, and 4 cases in the general ward. There were 7 boys and 2 girls; only 1 was a full-term neonate (37 weeks of gestation), and the remaining 8 were premature infants, the basic situation of children is shown in Table1.Table 1
Basic information of children with Raoulia ornithine solution sepsis.
CaseGenderGestational week (W)Cause of premature birthCesarean sectionBirth weight (kg)History of suffocationWhether it is a twin or multiple birthIs it a test tube babyAge at admission (d)Maternal pregnancy history1Male30 + 1Labor initiationYes1.4YesNoNo80G2P2, pregnancy-induced hypertension2Female27Onset of labor, massive bleeding from placenta previa, and premature rupture of membranesYes0.9YesNoNo68G5P2, hypothyroidism, GDM3Male32 + 6Premature rupture of membranesNo2.7NoNoNo3G2P24Female28 + 2Premature rupture of membranesNo0.9NoTwinsYes84G1P25Male35 + 2Placental abruptionYes2.4NoNoYes<1 (2 h)G5P16Male36 + 1Labor initiationNo3.3NoNoNo3G2P27Male37-No2.4UnknownNoNo15G2P28Male28 + 6Maternal cervical insufficiency and premature rupture of membranesYes1.45NoTripletsNo31G5P49Male33 + 3Onset of labor and premature rupture of membranesNo2.1YesNoNo<1 (6 h)G6P2Respiratory patterns before infection occurred in 9 patients: case 6 was ventilated by using a noninvasive ventilator, the case 4 was given high-flow oxygen, in case 8 was given oxygen by nasal cannula, and the remaining cases did not need oxygen therapy. The use of antibiotics before infection occurred in 9 children: 3 cases did not use antibiotics; the remaining 6 cases all using broad-spectrum antibiotics, including cefoperazone-sulbactam, meropenem, vancomycin, imipenem, cilastatin sodium, and linezolid from birth to the time of the infection and other antibiotics. The other 9 children all required intravenous nutrition; cases 1 and 6 had PICC intubation.


## 3.2. Blood Routine and Infection Index Monitoring

All the 9 patients had at least one or more abnormal indicators, and all the children had reduced platelets. In case 7, IL-6 was significantly elevated under normal conditions of other indicators. With effective anti-infective treatment, the levels of CRP and PCT in 7 children returned to normal, and the platelet count also gradually returned to normal. However, in case 4 and case 7, the inflammatory indicators did not decrease significantly or were at a continuous high value, and the platelet count gradually decreased or did not return to normal, as shown in Table2.Table 2
Changes of blood routine and inflammatory indexes in children with Raoultia.
CasesDays of infectionMonitoring timeWBC (×109•L−1)PLT (×109•L−1)Hb (g•L−1)NLCRP (g•L−1)PCT (μg•L−1)IL-6 (ng•L−1)1The same day9.1923.0681070.7370.23513.842.11>5000Day 29.208.22131110.5240.43394.1653.6—Day 39.2117.142730.6680.25397.1615.6826.66Day 49.227.6527950.3290.55431.897.5332.23Day 69.2416.2880950.590.28618.111.4922.48Day 99.2717.661061390.6790.2764.460.4410.53Day 119.3015.521481020.5190.40535.590.3613.172The same day8.238.8311990.610.25249.8519.82266Day 28.245.84720.6191.66245.6136.283356Day 38.256.226890.6920.236213.35——Day 48.266.121020.7360.22117.928.1771.34Day 68.2811.5614870.5870.22847.593.121.29Day 98.3118.17281250.6760.2075.890.415.63Day 169.78.15150910.510.28810.270.17.883The same day9.1412.463361250.780.71.9636.33Day 39.162.831121380.3530.6250.926.87>5000Day 49.1730.57171090.880.061103.43>100>5000Day 69.195628800.8640.0582.343.579.65Day 89.2147.38111460.6770.13215.7——Day 129.2515.97801130.6180.2366.460.343.064The same day9.23.872001270.5160.3986.431.75>5000Day 29.38.62151090.6550.21108.1935.421232Day 49.58.844650.7350.243170.7320.6730815The same day8.1515.32971130.8020.136106.7847.133948Day 28.1619.09811100.6570.19850.3212.51—Day 48.186.72146950.3910.32714.4——Day 98.237.68544990.2430.5471.760.14—Day 148.306.39390780.230.4980.94——Day 209.57.48315970.2850.5081.7——6The same day8.319.05303980.8680.06449.412.001191Day 39.25223850.4760.43270.261.81164.6Day 79.65.418740.6940.277116.4616.45311.9Day 89.713.9220950.5590.32881.446.232.52Day 109.911.921691030.5030.43113.30.61<1.5Day 149.136.77344970.3750.5161.80.11—7The same day9.78.58508920.5070.3941.890.223122Day 29.84.09208830.6950.262109.768.4542.2Day 39.93.84113870.4350.484163.66——Day 49.105.6932750.3820.476178.988.39Day 59.114.45191190.2570.639146.18——Day 69.129.2969970.3920.463100.8234.8523.16Day 79.1314.27820.7510.182101.6243.6140.7Day 89.1425.5472930.6850.20191.31——8The same day8.346.56135980.9590.01437.5810.57>5000Day 28.448.9922850.8250.101164.612.14842.5Day 38.535.19301450.7970.12184.664.4341.49Day 68.810.17891240.5980.24821.220.3825.93Day 18.147.322371010.4210.36620.111.959The same day9.166.782091260.8090.18952.84.53—Day 29.1724.36381040.6670.23615.9246.9634.04Day 39.1826.741001060.6130.28379.0525.183.42Day 59.2015.11230990.4180.48614.972<1.5Day 89.2311.22631930.3010.5851.590.223.54Day 139.2811.21578770.5820.2972.70.113.89


## 3.3. Clinical Features

Among the 9 children with Raoultia ornitholyticum septicemia, 7 had intestinal diseases, including 2 intestinal malformations and 5 neonatal necrotizing enterocolitis (NEC), of which 4 were had a history of intestinal surgery before Raoulia acidic infection. Among the other 9 cases, 2 cases had PICC catheter-related bloodstream infection, 2 cases had abnormal cerebrospinal fluid results and intracranial infection was considered, and 4 cases had different degrees of infection complications. In terms of clinical manifestations, 8 children had fever, of which 7 children showed repeated fever, and the remaining children showed changes in breathing, blood oxygen, and reaction. The length of hospital stay at the time of infection varies from 4 to 50 days, as shown in Table3 . In addition, after the occurrence of sepsis, 4 patients required invasive tracheal intubation for respiratory support, and 4 patients required oxygen therapy.Table 3
Clinical characteristics of 9 neonates with Raoulia.
CasesPrimary diseaseSurgery situationNumber of days of surgery at the time of infectionDays in the hospital at the time of infectionBacterial identificationClinical manifestationsWhether combined with intracranial infectionInfection complications1Ileal scarring strictures after NEC and premature infantsAdhesion bowel release, stricture bowel, and ileocecal resection819Blood and PICC catheter tipFever, shortness of breath, and nasal flaringNoLiver damage2NEC、BPD、 premature babyNo—7BloodDecreased blood oxygen and heart rateNoDIC3Congenital jejunal atresia (diaphragmatic type), enteric nerve dysplasia, and premature infantsEnteroplasty26BloodFever, poor response, frequent apnea, and decreased blood oxygenNoKidney damage4Premature infants and chronic lung diseaseNo—50BloodFever, poor mental response, visible markings all over the body, and vomiting of white mucus-like fluidYesSeptic shock, multiple organ dysfunction: Liver, kidney, myocardial damage, abnormal coagulation function, and ascites5Necrotizing enterocolitis in premature infants and neonatesNo—7BloodFeverYesNo6Congenital malrotation with midgut volvulus and intestinal necrosis and left testicular torsion with necrosisNecrotic bowel resection3132Blood and PICC lateral bloodFever and slightly poor mental responseNoNo7Neonatal necrotizing enterocolitisNo—4BloodRepeated fever for 9 daysNoNo8Neonatal necrotizing enterocolitis, premature infants, and BPDJejunostomy1819BloodFever and occasional transient oxygen desaturationNoNo9Aspiration pneumonia and premature infantsNo—27BloodFever with shallow and irregular breathingNoNo


## 3.4. Drug Susceptibility Results

14 strains were cocultured from 9 neonates with Raoultia ornitholyticum septicemia, 11 strains were carbapenem-resistant strains, of which 6 strains were resistant to levofloxacin, tigecycline, amikacin, and Compound sulfamethoxazole. 5 strains were only sensitive to tigecycline. The remaining 3 strains were sensitive strains, as shown in Table4.Table 4
Statistics of drug susceptibility results of 9 cases of neonatal Raoulia ornithinolytica sepsis.
CasesSpecimenPositive report time (h)Amoxicillin-clavulanate potassiumCefepimeCefoperazone-sulbactamCefoxitinCeftazidimeCeftriaxoneImipenemLevofloxacinPiperacillin-tazobactam sodiumTigecyclineAmikacinCotrimoxazole1Blood9RRRRRRRSRSSSBlood15RRRRRRRSRSSSPICC catheter tip—RRRRRRRSRSSSBlood17RRRRRRRSRSSS2Blood16RRRRRRRSRSSS3Blood16RRRRRRRSRSSS4Blood12RRRRRRRRRSRR5Blood17RRRRRRRRRSRRBlood53RRRRRRRRRSRR6Blood20RRRRRRRRRSRRPICC tube side blood12RRRRRRRRRSRR7Blood13SSSSSSSSSSSS8Blood15SSSSSSSSSSSS9Blood13SSSSSSSSSSSS


## 3.5. Anti-Infective Treatment and Outcome

Cases 1–6 are children with carbapenem-resistant bacteria infection. Among them, cases 1–3 were selected according to drug susceptibility to two sensitive drugs: the infection was effectively controlled by levofloxacin combined with amikacin treatment. In cases 5 and 6, the infection was also effectively controlled by removing the PICC catheter, increasing the dose of carbapenem, and prolonging the infusion time. Case 4 died of infection. Cases 7–9 were infected with susceptible strains, and cases 8 and 9 were effectively controlled by selecting sensitive drugs. In case 7, although a sensitive drug was selected for treatment, the effect was not good, and the parents of the child requested to be discharged from the hospital, as shown in Table5.Table 5
Anti-infective treatment and outcome of 9 cases of Raoulia ornitholyticum sepsis.
CasesUse anti-infective drugs and time of use (d)Other drug treatmentsTreatment outcome1Meropenem (1d), levofloxacin + amikacin (14 d)Removal of PICC tube, immunoglobulin, platelets, furosemide, packed red blood cells, frozen plasma, and human albuminCure2Meropenem (1d), levofloxacin + amikacin (14 d)Frozen plasma, furosemide, platelets, immunoglobulin, methylphenidate, packed red blood cells, and human serum albuminCure3Meropenem (2d), levofloxacin + amikacin + (14 d)Furosemide, human albumin, dopamine, platelets, and packed red blood cellsCure4Meropenem + amikacin (3 d)Immune globulin, platelets, packed red blood cells, frozen plasma, and furosemideDeath5Meropenem (40 mg/kg/time Q8H extended infusion time to 3 h, 21 d)ImmunoglobulinCure6Imipenem cilastatin sodium (25 mg/kg/time Q6H prolonged infusion time 2 h, 14 d)Remove the PICC tubeCure7Meropenem (40 mg/kg/time Q8H 8 d)Human immunoglobulin, furosemide, leukocyte-depleted suspended red blood cells, and plateletsUnknown (request for discharge)8Meropenem (14 d)NoCure9Meropenem + amikacin (14 d)NoCure


## 4. Discussions

Raoultia ornithinolytica is an aerobic, nonmotile, rod-shaped Gram-negative bacterium classified as Enterobacteriaceae of the genus Raoultella. This genus of bacteria also includes cytopathic Raoulia and Raoulia Tulsa. Raoultia is widely present in water, plants, soil and other environments, and mostly colonizes the digestive tract and upper respiratory tract in the human body, and is an opportunistic pathogen [9]. Invasive human infection of Raoulella ornithyloliticus is still rare. In recent years, the reports of Raoulella ornithyloliticus infection are more common in adults, and the reports of children infection, especially neonatal infection, are relatively rare [10]. Recently, Yaprak et al. [11] reported 14 cases of children infected with Raoulella ornithine, including 5 clinical cases, 3 of which were newborns, including 2 premature infants, and the results showed that all of them were bloodstream infections. Of the 9 infants enrolled in this study, 8 were premature infants. It shows that in the neonatal population, premature infants are at high risk of infection by Raoulia ornithine. Perhaps compared with the term infants, in addition to their low birth weight and less mature immune function, preterm infants often have multiple risk factors such as central venous catheterization, tracheal intubation, use of broad-spectrum antibiotics, parenteral nutrition, and nosocomial infection [12],which are all more likely to occur. In addition to preterm birth, among the 9 neonates with Raul Ornitholyticum septicemia analyzed in this paper, 7 neonates had intestinal diseases, and 4 of them had undergone gastrointestinal surgery, suggesting that neonates with intestinal problems or surgery may be more susceptible to infection with Raoulia ornithinolytica. This may be related to the fact that the bacteria are mainly localized in the digestive tract in the human body, and children with intestinal problems, such as NEC, often have impaired digestive gastrointestinal barrier function, which is easy to cause bacterial translocation and lead to infection.Neonatal sepsis is often subtle and nonspecific in clinical manifestations, and it is not easy to be detected, especially in very low birth weight (VLBW), which is more nonspecific and more difficult to identify early, which is also the anti-infective treatment for neonates [13]. The clinical manifestations of the 9 cases of neonatal Raoulia ornithinolytica septicemia in the author’s analysis were mostly only changes in respiration, blood oxygen, reaction, etc., and there was no specificity. This is mainly due to the production of histamine-like substances by L. ornithine, resulting in dyspnea and hypoxemia. However, it is worth noting that, in terms of systemic manifestations, 8 children had fever, suggesting that fever may be one of the clinical features of neonatal Raoulia ornithinolytica infection. In addition to close observation of clinical symptoms in children, early recognition of infection clinically can also be facilitated by assessing risk factors for infection in children and monitoring routine blood tests and infection markers [14, 15]. The combination of IL-6, PCT, and CRP is used to continuously and dynamically monitor high-risk groups of sepsis, which is of great significance for early detection and early treatment. Among them, IL-6 is the first elevated serum marker, and it often occurs when elevations occur before overt clinical symptoms [16, 17]. For example, the IL-6 of the child in case 7 was significantly elevated before clinical symptoms appeared and other infection markers were normal. Therefore, for children at high risk of infection, dynamic monitoring of CRP and PCT combined with IL-6 can help us identify, thereby winning an earlier treatment opportunity for anti-infective treatment. In addition, after initiating anti-infective treatment, dynamic monitoring of these infection markers will help us evaluate the efficacy and adjust the treatment plan in time. Maseda et al. [18] reported that PCT levels can be rapidly reduced after infection control, and septic patients can be reduced by 50% within 24 hours after effective treatment.Due to its special physiological characteristics and the toxic and side effects of drugs, neonates have very few drugs to choose from when facing CRE-resistant infection, which is another major difficulty in neonatal anti-infection treatment. In this study, 14 strains of Raoultia ornithinolytica isolated in this paper were highly resistant to the third and fourth generation cephalosporins, enzyme inhibitor compound preparations, and carbapenems. Eleven of them were carbapenem-resistant Enterobacteriaceae (CRE), which were only sensitive to aminoglycosides, quinolones, and tigecycline. In terms of anti-infective treatment, an anti-infective treatment plan should be formulated based on the basic situation of the child, the severity of infection, and drug susceptibility to achieve individualized treatment. For example, cases 1, 2, and 3 in this article showed that the PCT did not decrease significantly after 24–48 hours of meropenem treatment, suggesting that the curative effect may be poor. By changing the treatment plan in time, the infection of the three children was controlled. At the same time, case 5 had a large gestational age and birth weight, did not need oxygen therapy, and only had fever in clinical manifestations without other infection complications. Drug sensitivity results showed that the MIC value of imipenem and cilastatin sodium was 8ug/ml. According to relevant literature reports [19], in the treatment of CRE infection, when carbapenem MIC is 4–16 μg/ml in the treatment of CRE infection, carbapenem antibiotics should be used to increase the frequency or dose and prolong the infusion time. When carbapenems MIC> 16 dou g/ml, carbapenem antibiotics should be avoided. Taking meropenem into consideration, we chose meropenem for anti-infective treatment, increasing the drug dose to 40 mg/kg/time Q8H, optimizing the dosing schedule, and extending the infusion time of meropenem to 3 hours. In the end, the infection of the child was well controlled. In addition, case 6 was a PICC catheter-related infection. Through timely removal of the PICC catheter, increasing the dose of imipenem and cilastatin sodium (100 mg/kg/day, Q6H), and prolonging the drug infusion time to 2 hours, the child also achieved a good anti-infective treatment effect. In case 1, case 2, and case 3, meropenem was selected at the beginning, and then the anti-infective treatment regimen (levofloxacin and amikacin combined therapy) was adjusted promptly in combination with drug sensitivity. After 24–48 hours of treatment, the therapeutic effect was evaluated by strict monitoring of infection indicators. The results showed that all the three children achieved a good therapeutic effect, and no adverse drug reactions were detected. We know that aminoglycosides have ear and kidney toxicity, fluoroquinolones may cause joint and cartilage damage, and tigecycline may cause untoward reactions such as permanent tooth stain, enamel dysplasia, and bone growth inhibition, all of which limit the use of these drugs in the pediatric population [20]. However, when faced with a fatal infection, it should be used with caution after fully weighing the benefits and risks, and the adverse drug reactions should be closely monitored. Regrettably, in case 4, the child eventually developed septic shock and multiple organ dysfunction and died. The child was born very early, with an ultra-low birth weight, and had a variety of underlying diseases such as long-term need for oxygen therapy and extrauterine growth retardation, In addition, the child received multiple antibiotics from birth until infection. For such children, a nosocomial infection is fatal, so hand hygiene, rational use of antibiotics, protective isolation, and other nosocomial infection prevention and control measures are more important.In conclusion, Raoultella ornithinolytica sepsis in neonates occurred mainly through nosocomial infections and carbapenem-resistant strains were more common. Preterm birth, intestinal disease, and a history of surgery increase the risk of infection; for carbapenem-resistant Raoultella ornithinolytica infection, anti-infection treatment regimens should be formulated based on the basic situation, infection severity, and drug sensitivity of the children, so as to achieve individualized treatment. In addition, dynamic monitoring of infection markers has an important clinical significance for early identification of infection, evaluation of a curative effect, and timely adjustment of anti-infection treatment.



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