# Prevalence and Risk Factors of Childhood Hypertension in Urban-Rural Areas of China: A Cross-Sectional Study

Authors: Xiaohua Liang; Lun Xiao; Yetao Luo; Jiapei Xu
Journal: International Journal of Hypertension
 (2020)

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## Abstract

Objective. The increased blood pressure level in children and adolescents is recognized as an essential predictor of adulthood cardiovascular disease. This study aimed to ascertain the prevalence and the urban-rural disparity of childhood hypertension in the southwest of China. Methods. Using stratified cluster sampling in urban and rural areas, a total of 13597 primary school children aged 6∼12 years living in the Southwest of China were included. The prevalence of hypertension was analyzed. The risk factors were collected by questionnaires, and the risk factors of childhood hypertension were analyzed by the logistic regression model. Results. The prevalence of hypertension was 13.75%, 9.02%, and 17.47% in total, urban, and rural children, respectively, and the urban-rural difference was 8.44% (95%CI: 7.32%, 9.56%). Children with obesity, maternal gestational hypertension, >10 months of breastfeeding, or low family income had a significantly increased prevalence of hypertension (29.4%, 20.00%, 16.31%, and 16.25%, respectively). Rural residence, intake of more pickle (in rural), maternal gestational hypertension (in urban), low birth weight (in rural), obesity, increased heart rate, and red blood cell counts were the risk factors of childhood hypertension. The adjusted R2 values were 13.61%, 23.25%, 10.88%, 11.12%, 12.23%, and 25.04% in the full models excluding and including serum indexes for total, urban, and rural children, respectively. Conclusions. The prevalence of childhood hypertension is significant in the Southwest of China and alarming in rural areas, which requires community intervention. Children living in rural areas combined with obesity, low social economic status, dietary imbalance, and abnormal lipid metabolism were associated with an increased risk of hypertension, and routine care programs should be conducted to prevent childhood hypertension.


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## Body

## 1. Introduction

Elevated blood pressure level during childhood and adolescence is a recognized predictor of adulthood BP, leading to increased CVD disease in adults [1, 2]. It is imperative to study the prevalence and risk factors for childhood and adolescents’ hypertension, especially in low social economic status (SES) population. Despite the urban-rural differentials in BP have decreased in China, the BP level of rural children is constantly higher than that of urban children in China [3]. As well known, obesity is a stronger risk factor of childhood hypertension [4], and the urban-rural disparity of hypertension prevalence could not be explained by obesity alone [3], as the prevalence of childhood obesity in urban areas was higher than that of rural counterparts [5]. Obviously, it indicates that the risk factors of hypertension between urban and rural areas are different and other potential factors could provide further explanation. Previous studies have shown that higher BP levels were associated with a sedentary lifestyle [6], high dietary sodium intake [7], elevated low-density lipoprotein cholesterol (LDL-C), and high total cholesterol (TC) than those of their counterparts [8, 9]. These influencing factors are different in urban and rural areas. The dietary pattern of malnutrition could increase the risk of hypertension [10], and the dietary pattern was different between urban and rural children. In addition, children living in the city reported higher levels of physical activity than those living in rural areas [11]. Moreover, children living in urban areas usually have higher education levels and SES compared to those living in rural areas in China.The difference between urban and rural children in obesity, SES, dietary patterns, and physical activity was observed, and the prevalence of hypertension and the risk factors may vary between urban and rural areas. There are limited studies explored this relationship, and the present study will illustrate the prevalence of childhood hypertension and analyze the difference between urban and rural areas. This cross-sectional study with measures of anthropometric variables, dietary intake, physical activity, glycolipid indexes and blood cell composition measures, and perinatal factors in children provides an excellent opportunity to illustrate the differences of prevalence and the risk factors of hypertension between rural and urban areas in a large urban-rural population in Southwest of China. Furthermore, this study assesses to what extent these variables account for the level of variability in BP among rural and urban children aged 6–12 years.


## 2. Methods

### 2.1. Subjects

Two stages stratified (county and community) cluster sampling was used to choose participates. First, one urban county and one rural county were randomly selected from all urban and rural counties in Chongqing in Southwest of China, which represent urban and rural areas, respectively; second, two communities per county were randomly selected from all the communities of the two counties; and finally, all children living in the selected communities and studied in the local primary schools were included in the present study. A cross-sectional study was designed to assess the risk factors of childhood hypertension and their differences between urban and rural regions, with evaluations conducted in 2014. Inclusion criteria were as follows: (1) 6∼12 years old in 2014, (2) living in the survey areas for more than 6 months, (3) without serious diseases such as nephropathy, cardiovascular disease, and cancer, and (4) informed consent from both parents and children. This cross-sectional study planned to include 15 000 subjects, the rate of participate was 90.71%, and 13 597 samples were included in this study (in Supplementary Figure1). The approval for this study was agreed by the Institutional Review Board at the Children’s Hospital of Chongqing Medical University. Both children and parents provided informed consent. The teachers in the school submitted and collected the questionnaires.


### 2.2. Demographic Variables, Dietary Intake, and Physical Examination

Demographic variables, SES variables, prenatal variables, and dietary intake variables were collected, which were described in detail in previous publication [12]. Family history of parental obesity and hypertension was surveyed by questionnaires. In addition, physical activity (PA) was assessed by the self-reported activity categories (very heavy PA, heavy PA, medium PA, light PA, and sedentary lifestyle) and the duration of PA time per day, at weekday or weekend, spent on PA that was sufficient to “work up a sweat”. The average time per day of PA at weekday or weekend and overall PA time were used to analyze the children’s PA status. Moreover, dietary intake was surveyed by the self-reported food intake frequency questionnaire, and the details of dietary intake can be found in a previous study [13].Details of physical examination were introduced in a previous study [12]. A mobile medical ultrasonic machine (model WS-H300D) was used to measure height and weight, and BMI was calculated as weight/height2 (kg/m2). BP levels and heart rate were measured on three separate occasions with an OMRON arm-type electronic sphygmomanometer (HEM-7051). Children were diagnosed with hypertension if all three BP measurements met the criteria for hypertension, according the diagnose criteria of Chinese children [14]. Physical examinations and medical-history interviews were implemented by a pediatrician to exclude secondary hypertensive subjects.


### 2.3. Biochemical Indexes and Blood Cell Composition

A total of 5971 samples had biochemical index variables. Details of venous blood sample collection and measurement can be found in a previous study [12]. The serum glycolipid index was measured by an automatic biochemical analyzer (Mindray BS-800), and blood-cell composition was measured by automated hematology analyzers (Sysmex K-4500).


### 2.4. Diagnostic Criteria

The hypertension diagnostic criteria described by Mi Jie [15] were used in the present study, which were suitable for the growth characteristics of children in China. Hypertension was defined as mean clinic-measured SBP and/or DBP ≥95th percentile based on age, gender, and height percentiles. BP was measured by an electronic sphygmomanometer in this study. According to the sex-specific CDC BMI-for-age growth charts, overweight was diagnosed as 85th percentile ≤ BMI and BMI ≤ 95th percentile, and obesity was diagnosed as BMI ≥95th percentile [16].


### 2.5. Statistical Analyses

Normal distribution continuous variables of anthropometric variables, serum glycolipid indexes, and blood cell composition variables were reported as mean and standard deviation (SD), and Student'st-test was used to assess the differences between urban and rural areas. Skewed distribution continuous variables of dietary were expressed as median (interquartile range), and the Wilcoxon rank sum test was used to compare the difference between urban and rural areas. The prevalence of hypertension by categorical variables was reported as percentages of prevalence (%) and 95% confidence interval (CI), and the chi-squared test was used to test the difference between urban and rural areas. Moreover, the univariate logistic regression model was performed to analyze the relationship between hypertension and SES, perinatal measures, anthropometric measures, serum glycolipid indexes, blood cell composition, and dietary intake after adjusted age, gender, and height. Next, all of the significant variables were added to the multivariable logistic regression model to estimate their effects on childhood hypertension. Finally, all significant variables in the previous models were entered simultaneously to full model 1, and full model 2 added glycolipid and blood cell composition indexes based on full model 1.Statistical analysis was performed using SAS 9.4 software (Copyright © 2016 SAS Institute Inc., Cary, NC, USA). Ap value <0.05 was considered significant.


## 2.1. Subjects

Two stages stratified (county and community) cluster sampling was used to choose participates. First, one urban county and one rural county were randomly selected from all urban and rural counties in Chongqing in Southwest of China, which represent urban and rural areas, respectively; second, two communities per county were randomly selected from all the communities of the two counties; and finally, all children living in the selected communities and studied in the local primary schools were included in the present study. A cross-sectional study was designed to assess the risk factors of childhood hypertension and their differences between urban and rural regions, with evaluations conducted in 2014. Inclusion criteria were as follows: (1) 6∼12 years old in 2014, (2) living in the survey areas for more than 6 months, (3) without serious diseases such as nephropathy, cardiovascular disease, and cancer, and (4) informed consent from both parents and children. This cross-sectional study planned to include 15 000 subjects, the rate of participate was 90.71%, and 13 597 samples were included in this study (in Supplementary Figure1). The approval for this study was agreed by the Institutional Review Board at the Children’s Hospital of Chongqing Medical University. Both children and parents provided informed consent. The teachers in the school submitted and collected the questionnaires.


## 2.2. Demographic Variables, Dietary Intake, and Physical Examination

Demographic variables, SES variables, prenatal variables, and dietary intake variables were collected, which were described in detail in previous publication [12]. Family history of parental obesity and hypertension was surveyed by questionnaires. In addition, physical activity (PA) was assessed by the self-reported activity categories (very heavy PA, heavy PA, medium PA, light PA, and sedentary lifestyle) and the duration of PA time per day, at weekday or weekend, spent on PA that was sufficient to “work up a sweat”. The average time per day of PA at weekday or weekend and overall PA time were used to analyze the children’s PA status. Moreover, dietary intake was surveyed by the self-reported food intake frequency questionnaire, and the details of dietary intake can be found in a previous study [13].Details of physical examination were introduced in a previous study [12]. A mobile medical ultrasonic machine (model WS-H300D) was used to measure height and weight, and BMI was calculated as weight/height2 (kg/m2). BP levels and heart rate were measured on three separate occasions with an OMRON arm-type electronic sphygmomanometer (HEM-7051). Children were diagnosed with hypertension if all three BP measurements met the criteria for hypertension, according the diagnose criteria of Chinese children [14]. Physical examinations and medical-history interviews were implemented by a pediatrician to exclude secondary hypertensive subjects.


## 2.3. Biochemical Indexes and Blood Cell Composition

A total of 5971 samples had biochemical index variables. Details of venous blood sample collection and measurement can be found in a previous study [12]. The serum glycolipid index was measured by an automatic biochemical analyzer (Mindray BS-800), and blood-cell composition was measured by automated hematology analyzers (Sysmex K-4500).


## 2.4. Diagnostic Criteria

The hypertension diagnostic criteria described by Mi Jie [15] were used in the present study, which were suitable for the growth characteristics of children in China. Hypertension was defined as mean clinic-measured SBP and/or DBP ≥95th percentile based on age, gender, and height percentiles. BP was measured by an electronic sphygmomanometer in this study. According to the sex-specific CDC BMI-for-age growth charts, overweight was diagnosed as 85th percentile ≤ BMI and BMI ≤ 95th percentile, and obesity was diagnosed as BMI ≥95th percentile [16].


## 2.5. Statistical Analyses

Normal distribution continuous variables of anthropometric variables, serum glycolipid indexes, and blood cell composition variables were reported as mean and standard deviation (SD), and Student'st-test was used to assess the differences between urban and rural areas. Skewed distribution continuous variables of dietary were expressed as median (interquartile range), and the Wilcoxon rank sum test was used to compare the difference between urban and rural areas. The prevalence of hypertension by categorical variables was reported as percentages of prevalence (%) and 95% confidence interval (CI), and the chi-squared test was used to test the difference between urban and rural areas. Moreover, the univariate logistic regression model was performed to analyze the relationship between hypertension and SES, perinatal measures, anthropometric measures, serum glycolipid indexes, blood cell composition, and dietary intake after adjusted age, gender, and height. Next, all of the significant variables were added to the multivariable logistic regression model to estimate their effects on childhood hypertension. Finally, all significant variables in the previous models were entered simultaneously to full model 1, and full model 2 added glycolipid and blood cell composition indexes based on full model 1.Statistical analysis was performed using SAS 9.4 software (Copyright © 2016 SAS Institute Inc., Cary, NC, USA). Ap value <0.05 was considered significant.


## 3. Results

### 3.1. General Characteristics

The general characteristics of the subjects are presented in Table1. The mean age was 9.23 ± 1.76 years, and 51.92% (7060/13597) were males. Compared with children from urban areas, children living in rural areas had significantly elevated age, heart rate, FGB, TC, TG, HDL-C, LDL-C, white blood cell (WBC), red blood cell (RBC), hemoglobin (HGB), hematocrit (HCT), mean corpuscular volume (MCV), WBC small cell ratio (WSCR), WBC small cell count or lymphocyte count (WSCC), red blood cell distribution width (RDWSD), mean platelet volume (MPV), and the percent of living with grandparents, but significantly reduced BMI, weight, waist circumference, MCHC, PLT, WMCR, WMCC, WLCC, PA, and father with obesity (all P<0.05, Table 1). There was significant difference in the percent of father’s education, parental occupation, income, people live with child, birth weight, and breastfeeding between the urban and rural groups. In addition, in Supplementary Table 1, the average intake amount of fruit, fish, eggs, milk, bean food, nuts, mushrooms and algae food, and vegetables was lower for children living in rural areas than those living in urban areas (all P<0.05), and the average intake of cereals and potatoes, pickle, and nutritional supplements was higher for children living in rural areas than its counterparts (P<0.05).Table 1
General characteristics of children between urban and rural areas.
Variables
Total
Urban vs rural
Urban (n = 5995)
Rural (n = 7602)
P
Anthropometric measures
Gender, male (n (%))
7060 (51.92%)
3098 (51.68%)
3962 (52.12%)
0.609
Age, y
9.24 ± 1.76
9.05 ± 1.76
9.39 ± 1.74
<0.001
BMI, kg/m2
17.35 ± 3.05
17.53 ± 3.02
17.22 ± 3.07
<0.001
Height, cm
134.51 ± 11.5
134.66 ± 11.44
134.39 ± 11.56
0.182
Weight, kg
32.01 ± 9.49
32.40 ± 9.53
31.7 ± 9.45
<0.001
Waist circumference, cm
58.12 ± 8.49
59.17 ± 8.73
57.3 ± 8.21
<0.001
Heart rate, n/min
95.28 ± 12.72
94.95 ± 12.71
95.54 ± 12.72
0.007
Serum biochemical index
FBG, mmol/L
4.14 ± 0.62
3.94 ± 0.50
4.90 ± 0.46
<0.001
TC, mmol/L
3.49 ± 0.66
3.34 ± 0.57
4.10 ± 0.63
<0.001
TG, mmol/L
0.97 ± 0.58
0.96 ± 0.58
1.03 ± 0.57
<0.001
HDL-C, mmol/L
1.22 ± 0.27
1.19 ± 0.26
1.35 ± 0.28
<0.001
LDL-C, mmol/L
1.72 ± 0.54
1.56 ± 0.39
2.35 ± 0.60
<0.001
Blood cell composition measures
WBC,n × 10∧9/L
7.12 ± 1.79
7.12 ± 1.77
7.16 ± 1.85
0.480
RBC,n × 10∧12/L
4.59 ± 0.39
4.52 ± 0.35
4.90 ± 0.40
<0.001
HGB, g/L
126.42 ± 9.98
124.31 ± 8.56
134.73 ± 10.82
<0.001
HCT, %
37.66 ± 3.11
36.74 ± 2.43
41.29 ± 2.78
<0.001
MCV, fL
81.91 ± 6.44
81.37 ± 5.98
84.01 ± 7.66
<0.001
MCH, pg
27.65 ± 1.95
27.64 ± 1.94
27.69 ± 1.96
0.390
MCHC, g/L
336.58 ± 14.98
338.99 ± 15.08
327.11 ± 10.06
<0.001
PLT,n × 10∧9/L
287.48 ± 67.49
288.53 ± 67.42
283.35 ± 67.63
0.020
WSCR, %
36.11 ± 8.25
35.59 ± 8.03
38.15 ± 8.80
<0.001
WMCR, %
7.05 ± 2.75
7.41 ± 2.87
5.64 ± 1.60
<0.001
WSCC,n × 10∧9/L
2.52 ± 0.66
2.47 ± 0.63
2.70 ± 0.73
<0.001
WMCC,n × 10∧9/L
0.51 ± 0.34
0.53 ± 0.28
0.44 ± 0.48
<0.001
WLCC,n × 10∧9/L
4.08 ± 1.49
4.12 ± 1.48
3.90 ± 1.50
<0.001
RDWSD, %
34.59 ± 4.82
33.40 ± 4.23
39.27 ± 4.09
<0.001
PDW, fL
13.47 ± 2.37
13.48 ± 2.32
13.39 ± 2.57
0.207
MPV, fL
10.68 ± 1.30
10.57 ± 1.32
11.11 ± 1.15
<0.001
P-LCR, %
32.67 ± 8.65
32.66 ± 8.86
32.69 ± 7.76
0.910
Socioeconomic index
Father’s education level, y
∼9
6856 (51.75%)
2190 (37.80%)
4666 (62.58%)
<0.001
∼12
4915 (37.10%)
2787 (48.11%)
2128 (28.54%)
∼15
1390 (10.49%)
755 (13.03%)
635 (8.52%)
>15
88 (0.66%)
61 (1.05%)
27 (0.36%)
Father’s occupation
Manager
933 (7.02%)
389 (6.70%)
544 (7.26%)
<0.001
Worker
4016 (30.21%)
1733 (29.84%)
2283 (30.49%)
Technicist/researcher
571 (4.29%)
380 (6.54%)
191 (2.55%)
Farmer
4593 (34.55%)
1686 (29.03%)
2907 (38.82%)
Others
3182 (23.93%)
1619 (27.88%)
1563 (20.87%)
Mother’s occupation
Manager
605 (4.55%)
241 (4.12%)
364 (4.88%)
<0.001
Worker
3538 (26.58%)
1666 (28.45%)
1872 (25.11%)
Technicist/researcher
239 (1.80%)
132 (2.25%)
107 (1.44%)
Farmer
4179 (31.40%)
2111 (36.05%)
2068 (27.74%)
Others
4750 (35.68%)
1705 (29.12%)
3045 (40.84%)
Income, RMB
∼500
738 (6.25%)
92 (2.05%)
646 (8.83%)
<0.001
∼1000
1395 (11.82%)
235 (5.23%)
1160 (15.86%)
∼2000
2327 (19.72%)
627 (13.96%)
1700 (23.25%)
∼3000
2750 (23.30%)
1051 (23.41%)
1699 (23.24%)
>3000
4592 (38.91%)
2485 (55.35%)
2107 (28.82%)
Living with grandparents
No
10060 (84.76%)
3992 (88.42%)
6068 (82.51%)
<0.001
Yes
1809 (15.24%)
523 (11.58%)
1286 (17.49%)
People live with child
1
583 (4.98%)
154 (3.43%)
429 (5.94%)
<0.001
2∼3
6114 (52.22%)
2595 (57.81%)
3519 (48.74%)
4
5012 (42.80%)
1740 (38.76%)
3272 (45.32%)
Medical insurance
No
1790 (15.14%)
383 (8.50%)
1407 (19.24%)
<0.001
Yes
10030 (84.86%)
4124 (91.50%)
5906 (80.76%)
Perinatal measures
Gestational hypertension
No
11299 (98.47%)
4366 (98.76%)
6933 (98.30%)
0.052
Yes
175 (1.53%)
55 (1.24%)
120 (1.70%)
Birth weight, g
∼3000
3181 (26.86%)
1133 (25.16%)
2048 (27.91%)
<0.001
∼3600
5051 (42.65%)
2058 (45.70%)
2993 (40.78%)
>3600
3610 (30.48%)
1312 (29.14%)
2298 (31.31%)
Breastfeeding, month
0∼3
2845 (24.40%)
1420 (31.77%)
1425 (19.81%)
<0.001
4∼10
5555 (47.64%)
2223 (49.74%)
3332 (46.33%)
>10
3261 (27.97%)
826 (18.48%)
2435 (33.86%)
Father with obesity
No
9839 (83.89%)
3703 (82.79%)
6136 (84.56%)
0.011
Yes
1890 (16.11%)
770 (17.21%)
1120 (15.44%)
Mother with obesity
No
10505 (89.57%)
4017 (89.73%)
6488 (89.48%)
0.670
Yes
1223 (10.43%)
460 (10.27%)
763 (10.52%)
Physical activity
Weekday, min/day
∼10
6321 (53.41%)
2246 (49.87%)
4075 (55.59%)
<0.001
>10
5514 (46.59%)
2258 (50.13%)
3256 (44.41%)
Weekend, min/day
∼55
7782 (65.77%)
2795 (62.11%)
4987 (68.01%)
<0.001
>55
4051 (34.23%)
1705 (37.89%)
2346 (31.99%)
Total, min/week
∼150
6210 (52.59%)
2170 (48.32%)
4040 (55.21%)
<0.001
>150
5599 (47.41%)
2321 (51.68%)
3278 (44.79%)
BMI, body mass index; FBG, fasting blood glucose; TC, total cholesterol; TG, triglyceride; HDL-C, high-density lipoprotein cholesterol; LDL-C, low-density lipoprotein cholesterol; WBC, white blood cell; RBC, red blood cell; HGB, hemoglobin; HCT, hematocrit; MCV, mean corpuscular volume; MCH, mean corpuscular hemoglobin; MCHC, mean corpuscular hemoglobin concentration; PLT, platelet; WSCR, WBC small cell ratio; WMCR, WBC middle cell ratio or mixed cell percent; WSCC, WBC small cell count or lymphocyte count; WMCC, WBC middle cell count or mixed cell count; WLCC, WBC large cell count or neutrophil count; RDWSD, red blood cell distribution width; PDW, platelet distribution width; MPV, mean platelet volume; P-LCR, platelet-large cell ratio.


### 3.2. Prevalence of Childhood Hypertension

Overall, the prevalence of hypertension in 6∼12-year-old children was 13.75%, and there was significant difference between urban and rural areas (8.44% (7.32%, 9.56%),P<0.001) (Table 2). The prevalence was elevated in children with the characteristics of living in rural areas, younger age group, obesity, low family income, living with grandparents, no medical insurance, maternal gestational hypertension, low birth weight (LBW), >10 months of breastfeeding, parental obesity, and low PA (all P<0.05) (Table 2). In the subgroup analyses by areas, the prevalence of hypertension in children with maternal gestational hypertension was markedly elevated in urban areas (P<0.05) but not in rural areas (Table 2). The prevalence was higher for children with the characteristics of younger age group and overweight/obesity, in both urban and rural areas. In the subgroup analyses of age, obesity, income, living with grandparents, people live with child, medical insurance, birth weight, breast feeding, father with obesity, mother with obesity, and PA, the prevalence of hypertension in rural children was higher than those of urban children (all P<0.05) (Table 2).Table 2
Prevalence of hypertension of children aged 6–12 years.
Variables
Total
Urban
Rural
Difference (rural vs urban)
P
Prevalence
P
Prevalence
P
Prevalence
P
Total
13.75% (13.17%, 14.34%)
9.02% (8.31%, 9.78%)
17.47% (16.62%, 18.34%)
8.44% (7.32%, 9.56%)
<0.001
Anthropometric measures
Gender
Male
13.50% (12.71%, 14.32%)
0.385
8.72% (7.75%, 9.76%)
0.388
17.24% (16.07%, 18.45%)
0.581
8.52% (6.98%, 10.06%)
<0.001
Female
14.01% (13.18%, 14.88%)
9.35% (8.32%, 10.47%)
17.72% (16.49%, 19.00%)
8.37% (6.73%, 10.00%)
<0.001
Age, y
6
16.73% (14.92%, 18.66%)
<0.001
14.15% (11.83%, 16.72%)
<0.001
19.50% (16.75%, 22.49%)
<0.001
5.36% (1.67%, 9.04%)
0.004
7
15.66% (14.26%, 17.15%)
10.91% (9.23%, 12.79%)
20.35% (18.15%, 22.69%)
9.44% (6.61%, 12.26%)
<0.001
8
13.70% (12.32%, 15.17%)
7.30% (5.79%, 9.05%)
18.97% (16.85%, 21.24%)
11.67% (8.99%, 14.35%)
<0.001
9
11.68% (10.34%, 13.13%)
8.16% (6.47%, 10.12%)
14.41% (12.46%, 16.53%)
6.25% (3.58%, 8.91%)
<0.001
10
12.25% (10.90%, 13.70%)
7.31% (5.65%, 9.28%)
15.39% (13.49%, 17.44%)
8.08% (5.47%, 10.69%)
<0.001
11
13.28% (11.89%, 14.77%)
6.37% (4.84%,8.22%)
17.72% (15.71%, 19.87%)
11.35%(8.74%,13.96%)
<0.001
12
12.69% (10.35%, 15.33%)
8.24% (5.24%, 12.21%)
15.28% (12.11%, 18.91%)
7.04% (2.38%, 11.71%)
0.003
Obesity
Normal
11.33% (10.70%, 11.99%)
<0.001
7.01% (6.24%, 7.84%)
<0.001
14.59% (13.65%, 15.56%)
<0.001
7.57% (6.35%, 8.80%)
<0.001
Overweight
15.50% (14.24%, 16.83%)
10.16% (8.63%, 11.85%)
20.04%(18.14%,22.04%)
9.88%(7.39%,12.37%)
<0.001
Obesity
29.40% (26.73%, 32.17%)
21.23% (17.84%, 24.93%)
37.06% (33.09%, 41.17%)
15.83% (10.58%, 21.09%)
<0.001
Socioeconomic index
Father’s education level, ya
∼9
14.22% (13.40%, 15.07%)
0.256
8.36% (7.23%, 9.59%)
0.098
16.97% (15.91%, 18.08%)
0.465
8.62% (7.04%, 10.20%)
<0.001
∼12
13.06% (12.13%, 14.04%)
8.97% (7.93%, 10.09%)
18.42% (16.79%, 20.13%)
9.45% (7.49%, 11.41%)
<0.001
∼15
13.81% (12.04%, 15.74%)
10.73% (8.61%, 13.16%)
17.48% (14.60%, 20.66%)
6.75% (3.06%, 10.44%)
<0.001
>15
17.05% (9.87%, 26.55%)
14.75% (6.98%, 26.17%)
22.22% (8.62%, 42.26%)
7.47% (−10.56%, 25.5%)
0.417
Father’s occupationb
Manager
13.29% (11.18%, 15.64%)
0.110
11.31% (8.34%, 14.89%)
0.007
14.71% (11.84%, 17.97%)
0.006
3.39% (−0.94%, 7.73%)
0.125
Worker
14.82% (13.73%, 15.95%)
8.25% (7.00%, 9.65%)
19.80% (18.18%, 21.49%)
11.55% (9.46%, 13.63%)
<0.001
Technicist/researcher
13.84% (11.11%, 16.94%)
13.42% (10.16%, 17.27%)
14.66% (9.97%, 20.49%)
1.24% (−4.84%, 7.31%)
0.689
Farmer
13.89% (12.90%, 14.92%)
8.24% (6.98%, 9.66%)
17.17% (15.81%, 18.59%)
8.92% (7.02%, 10.82%)
<0.001
Others
12.60% (11.47%, 13.81%)
9.20% (7.84%, 10.72%)
16.12% (14.33%, 18.04%)
6.92% (4.62%, 9.22%)
<0.001
Mother’s occupationc
Manager
13.76% (11.11%, 16.77%)
0.096
10.79% (7.17%, 15.41%)
0.104
15.75% (12.15%, 19.91%)
0.268
4.87% (−0.54%, 10.28%)
0.078
Worker
14.32% (13.18%, 15.52%)
8.84% (7.51%, 10.31%)
19.16% (17.40%, 21.01%)
10.24% (8.00%, 12.49%)
<0.001
Technicist/researcher
16.74% (12.23%, 22.09%)
15.15% (9.51%, 22.43%)
18.69% (11.81%, 27.38%)
3.54% (−6.05%, 13.13%)
0.469
Farmer
12.74% (11.74%, 13.79%)
8.79% (7.61%, 10.09%)
16.73% (15.15%, 18.41%)
8.06% (6.06%, 10.07%)
<0.001
Others
14.26% (13.27%, 15.29%)
8.62% (7.33%, 10.06%)
17.40% (16.06%, 18.79%)
8.78% (6.89%, 10.68%)
<0.001
Income, RMBd
∼500
16.26% (13.67%, 19.12%)
0.009
9.78% (4.57%, 17.76%)
0.880
17.18% (14.35%, 20.32%)
0.988
7.40% (0.67%, 14.13%)
0.031
∼1000
16.06% (14.17%, 18.09%)
8.51% (5.28%, 12.84%)
17.59% (15.44%, 19.90%)
9.08% (4.89%, 13.26%)
<0.001
∼2000
15.13% (13.69%, 16.65%)
8.29% (6.26%, 10.73%)
17.65% (15.86%, 19.54%)
9.35% (6.54%, 12.17%)
<0.001
∼3000
14.33% (13.04%, 15.69%)
8.85% (7.20%, 10.73%)
17.72% (15.93%, 19.62%)
8.87% (6.37%, 11.37%)
<0.001
>3000
13.00% (12.04%, 14.01%)
9.50% (8.37%, 10.72%)
17.13% (15.55%, 18.81%)
7.64% (5.66%, 9.62%)
<0.001
Living with grandparentse
No
13.97% (13.29%, 14.66%)
0.039
9.27% (8.39%, 10.21%)
0.355
17.06% (16.12%, 18.03%)
0.099
7.79% (6.48%, 9.09%)
<0.001
Yes
15.81% (14.16%, 17.57%)
8.03% (5.85%, 10.70%)
18.97% (16.86%, 21.23%)
10.94% (7.78%, 14.11%)
<0.001
People live with childf
1
15.44% (12.60%, 18.63%)
0.106
9.09% (5.06%, 14.78%)
0.687
17.72% (14.22%, 21.66%)
0.802
8.62% (2.82%, 14.43%)
0.004
2∼3
13.58% (12.73%, 14.46%)
8.82% (7.76%, 9.98%)
17.08% (15.85%, 18.36%)
8.25% (6.60%, 9.91%)
<0.001
4
14.86% (13.89%, 15.88%)
9.60% (8.25%, 11.08%)
17.67% (16.37%, 19.02%)
8.07% (6.16%, 9.97%)
<0.001
Medical insuranceg
No
13.83% (13.16%, 14.52%)
0.004
9.26% (8.39%, 10.19%)
0.209
17.01% (16.06%, 17.99%)
0.096
11.59% (8.28%, 14.91%)
<0.001
Yes
16.41% (14.72%, 18.20%)
7.31% (4.91%, 10.39%)
18.88% (16.87%, 21.02%)
7.79% (6.49%, 9.10%)
<0.001
Perinatal measures
Gestational hypertensionh
No
14.02% (13.38%, 14.67%)
0.024
8.96% (8.12%, 9.84%)
0.018
17.21% (16.33%, 18.12%)
0.297
8.25% (7.02%, 9.48%)
<0.001
Yes
20.00% (14.34%, 26.70%)
18.18% (9.08%, 30.90%)
20.83% (13.96%, 29.20%)
2.65% (−9.87%, 15.17%)
0.678
Birth weight, gi
∼3000
15.59% (14.35%, 16.90%)
0.026
9.89% (8.21%, 11.77%)
0.110
18.75% (17.08%, 20.51%)
0.149
8.86% (6.44%, 11.29%)
<0.001
∼3600
14.04% (13.09%, 15.03%)
9.52% (8.29%, 10.87%)
17.14% (15.81%, 18.54%)
7.62% (5.76%, 9.47%)
<0.001
>3600
13.35% (12.26%, 14.50%)
7.70% (6.31%, 9.28%)
16.58% (15.08%, 18.16%)
8.88% (6.79%, 10.98%)
<0.001
Breastfeeding, monthj
0∼3
13.74% (12.50%, 15.06%)
<0.001
10.42% (8.88%, 12.13%)
0.090
17.05% (15.13%, 19.11%)
0.084
6.63% (4.11%, 9.15%)
<0.001
4∼10
13.21% (12.33%, 14.13%)
8.28% (7.16%, 9.50%)
16.51% (15.26%, 17.81%)
8.23% (6.53%, 9.93%)
<0.001
>10
16.31% (15.06%, 17.63%)
9.20% (7.32%, 11.38%)
18.73% (17.20%, 20.33%)
9.53% (7.02%, 12.03%)
<0.001
Father with obesityk
No
14.02% (13.36%, 14.70%)
0.149
8.99% (8.12%, 9.91%)
0.126
17.14% (16.23%, 18.08%)
0.263
8.42% (7.10%, 9.73%)
<0.001
Yes
16.19% (14.17%, 18.38%)
10.00% (7.41%, 13.11%)
19.92% (17.14%, 22.93%)
8.05% (4.91%, 11.20%)
<0.001
Mother with obesityl
No
14.03% (13.35%, 14.73%)
0.040
8.78% (7.88%, 9.73%)
0.474
17.19% (16.26%, 18.16%)
0.055
8.15% (6.88%, 9.43%)
<0.001
Yes
15.29% (13.70%, 16.99%)
10.52% (8.44%, 12.90%)
18.57% (16.33%, 20.98%)
9.92% (5.98%, 13.86%)
<0.001
Physical activity
Weekday, min/daym
∼10
14.89% (14.02%, 15.79%)
0.028
9.26% (8.09%, 10.54%)
0.637
17.99% (16.82%, 19.20%)
0.141
8.73% (7.05%, 10.41%)
<0.001
>10
13.47% (12.58%, 14.40%)
8.86% (7.72%, 10.11%)
16.68% (15.41%, 18.00%)
7.82% (6.08%, 9.56%)
<0.001
Weekend, min/dayn
∼55
14.37% (13.59%, 15.17%)
0.560
8.91% (7.88%, 10.03%)
0.590
17.43% (16.38%, 18.51%)
0.900
8.52% (7.03%, 10.01%)
<0.001
>55
13.97% (12.92%, 15.08%)
9.38% (8.04%, 10.87%)
17.31% (15.80%, 18.90%)
7.92% (5.86%, 9.99%)
<0.001
Total, min/weeko
∼150
14.83% (13.96%, 15.74%)
0.050
9.17% (7.99%, 10.46%)
0.808
17.87% (16.70%, 19.09%)
0.247
8.70% (7.01%, 10.39%)
<0.001
>150
13.57% (12.69%, 14.50%)
8.96% (7.83%, 10.20%)
16.84% (15.57%, 18.17%)
7.88% (6.15%, 9.61%)
<0.001
aA total of 348 subjects having missing values in father’s education level; bA total of 302 subjects having missing values in father’s occupation. cA total of 286 subjects having missing values in mother’s occupation. dA total of 1795 subjects having missing values in parents’ income. eA total of 1728 subjects having missing values in living with grandparents. fA total of 1888 subjects having missing values in people live with child. gA total of 1777 subjects having missing values in medical insurance. hA total of 2123 subjects having missing values in gestational hypertension. iA total of 1755 subjects having missing values in birth weight. jA total of 1936 subjects having missing values in breastfeeding. kA total of 1868 subjects having missing values in father with obesity. lA total of 1869 subjects having missing values in mother with obesity. mA total of 1762 subjects having missing values in weekday activity. nA total of 1764 subjects having missing values in weekend activity. oA total of 1788 subjects having missing values in total activityThe prevalence of childhood hypertension was also analyzed in relation to pairs of the following variables: birth weight, duration of breastfeeding, family income, obesity, and living with grandparents. In Figure1 and Figure 2, combinations of the above risk factors led to significantly higher risks for HTN in both urban and rural areas, it is shown in Figures 1 and 2 and Supplementary Tables 2–5. In Figure 1(a), children with >10 months of breastfeeding duration and living with grandparents had a significantly higher prevalence of hypertension than other groups (16.98%, P<0.05) in urban regions but not in rural regions. In addition, obese children with 0∼3 months of breastfeeding duration or LBW (<3000 g) have the highest prevalence of hypertension in urban regions (25.19%, P<0.001; 22.37%, P<0.001) (Figures 1(c) and 2(c)), while obese children with >10 months of breastfeeding duration or LBW (<3000 g) have the highest prevalence of hypertension in rural regions (39.80%, P<0.001; 43.80%, P<0.001) (in Figure 1(d) and Figure 2(d)).Figure 1
Prevalence of hypertension for children having different durations of breastfeeding combined with other characteristics. (a). Prevalence of hypertension for children combined with duration of breastfeeding and living with grandparents in urban regions. (b). Prevalence of hypertension for children combined with duration of breastfeeding and living with grandparents in rural regions. (c). Prevalence of hypertension for children combined with duration of breastfeeding and obesity in urban regions. (d). Prevalence of hypertension for children combined with duration of breastfeeding and obesity in rural regions. (e). Prevalence of hypertension for children combined with duration of breastfeeding and birth weight in urban regions. (f) Prevalence of hypertension for children combined with duration of breastfeeding and birth weight in rural regions.
(a)
(b)
(c)
(d)
(e)
(f)Figure 2
Prevalence of hypertension for children combined with two characteristics. (a). Prevalence of hypertension for children combined with present weight and living with grandparents in urban regions. (b). Prevalence of hypertension for children combined with present weight and living with grandparents in rural regions. (c). Prevalence of hypertension for children combined with birth weight and present weight in urban regions. (d). Prevalence of hypertension for children combined with birth weight and present weight in rural regions. (e). Prevalence of hypertension for children combined with income and present weight in urban regions. (f). Prevalence of hypertension for children combined with income and present weight in rural regions.
(a)
(b)
(c)
(d)
(e)
(f)


### 3.3. Using Univariate Model to Analyze the Risk Factors of Childhood Hypertension

The results of the univariate logistic regression model, adjusting age, sex, and height, illustrated that overweight/obesity, maternal gestational hypertension, >10 months of breastfeeding, maternal obesity, less weekday PA, and more pickle intake were risk factors for childhood hypertensive (allP<0.05), whereas a paternal education level of 9∼12 years (ref. ≤ 9 y), high income, and increased birth weight were protective factors against childhood hypertensive (all P<0.05) (Table 3). In the subgroup analyses, maternal gestational hypertension was a predictor of hypertension for urban children, while >10 months of breastfeeding and pickle intake effect the BP level only in rural areas (P<0.05).Table 3
Results of the univariate logistic model of the risk factors for childhood hypertension.
Variablesa
Total
Urban
Rural
β
OR (95% CI)
P
β
OR (95% CI)
P
β
OR (95% CI)
P
Region (urban vs. rural)
−0.209
0.811 (0.707, 0.931)
0.003
Obesity, ref. normal
Overweight
0.404
1.497 (1.330, 1.686)
<0.001
0.446
1.563 (1.260, 1.938)
<0.001
0.395
1.484 (1.285, 1.714)
<0.001
Obesity
1.283
3.608 (3.095, 4.206)
<0.001
1.354
3.872 (2.988, 5.017)
<0.001
1.276
3.584 (2.946, 4.359)
<0.001
Education, ref. ≤9 y
∼12
−0.137
0.872 (0.782, 0.972)
0.014
−0.003
0.997 (0.815, 1.221)
0.981
0.068
1.070 (0.936, 1.225)
0.322
∼15
−0.087
0.917 (0.775, 1.085)
0.313
0.166
1.181 (0.893, 1.562)
0.244
−0.017
0.983 (0.789, 1.225)
0.877
>15
0.156
1.169 (0.667, 2.049)
0.585
0.523
1.687 (0.814, 3.497)
0.159
0.306
1.358 (0.545, 3.383)
0.511
Father’s occupation, ref. worker
Manager
−0.141
0.869 (0.705, 1.071)
0.187
0.326
1.386 (0.965, 1.990)
0.077
−0.384
0.681 (0.525, 0.883)
0.004
Technicist/researcher
−0.108
0.898 (0.697, 1.157)
0.404
0.498
1.646 (1.168, 2.318)
0.004
−0.377
0.686 (0.453, 1.039)
0.075
Farmer
−0.078
0.925 (0.819, 1.045)
0.209
0.003
1.003 (0.785, 1.282)
0.981
−0.179
0.836 (0.725, 0.963)
0.013
Others
−0.194
0.824 (0.719, 0.944)
0.005
0.106
1.112 (0.874, 1.414)
0.390
−0.251
0.778 (0.657, 0.923)
0.004
Mother’s occupation, ref. worker
Manager
−0.051
0.950 (0.740, 1.220)
0.688
0.219
1.244 (0.799, 1.937)
0.333
−0.268
0.765 (0.563, 1.038)
0.086
Technicist/researcher
0.153
1.165 (0.819, 1.659)
0.396
0.531
1.701 (1.024, 2.825)
0.040
−0.061
0.940 (0.570, 1.551)
0.810
Farmer
−0.141
0.868 (0.761, 0.990)
0.035
−0.035
0.965 (0.769, 1.212)
0.762
−0.155
0.856 (0.727, 1.008)
0.063
Others
−0.002
0.998 (0.881, 1.131)
0.977
−0.015
0.985 (0.774, 1.254)
0.905
−0.117
0.890 (0.767, 1.033)
0.125
Income, ref. ≤500 RMB
∼1000
−0.012
0.988 (0.775, 1.259)
0.920
−0.131
0.877 (0.383, 2.009)
0.757
0.026
1.027 (0.796, 1.325)
0.839
∼2000
−0.092
0.912 (0.727, 1.144)
0.428
−0.180
0.835 (0.396, 1.763)
0.637
0.028
1.029 (0.809, 1.308)
0.819
∼3000
−0.164
0.849 (0.679, 1.062)
0.151
−0.113
0.893 (0.433, 1.841)
0.759
0.019
1.019 (0.801, 1.296)
0.878
>3000
−0.297
0.743 (0.599, 0.921)
0.007
−0.081
0.922 (0.456, 1.866)
0.822
−0.027
0.973 (0.770, 1.231)
0.822
Living with grandparents (yes vs. no)
0.130
1.139 (0.991, 1.309)
0.066
−0.228
0.796 (0.568, 1.116)
0.186
0.120
1.127 (0.965, 1.316)
0.131
People live with child, ref. 2∼3
1
0.165
1.179 (0.931, 1.494)
0.172
0.067
1.069 (0.606, 1.886)
0.817
0.051
1.052 (0.809, 1.370)
0.704
4
0.099
1.104 (0.992, 1.229)
0.070
0.069
1.071 (0.868, 1.322)
0.522
0.035
1.035 (0.913, 1.175)
0.589
Medical insurance (yes vs. no)
−0.209
0.811 (0.707, 0.931)
0.003
0.270
1.310 (0.878, 1.954)
0.187
−0.131
0.877 (0.755, 1.019)
0.087
Gestational hypertension (yes vs. no)
0.422
1.525 (1.049, 2.218)
0.027
0.820
2.270 (1.132, 4.550)
0.021
0.232
1.261 (0.808, 1.970)
0.308
Birth weight, 100g
−0.013
0.987 (0.976, 0.998)
0.018
−0.020
0.980 (0.959, 1.002)
0.076
−0.010
0.990 (0.978, 1.003)
0.137
Breastfeeding, ref. 4∼10 months
0∼3
0.025
1.025 (0.898, 1.170)
0.714
0.223
1.250 (0.995, 1.572)
0.056
0.019
1.020 (0.863, 1.204)
0.819
>10
0.271
1.312 (1.161, 1.481)
<0.001
0.157
1.170 (0.883, 1.551)
0.275
0.175
1.191 (1.038, 1.367)
0.013
Father with obesity (yes vs. no)
0.088
1.092 (0.951, 1.254)
0.211
0.167
1.182 (0.913, 1.531)
0.205
0.072
1.075 (0.911, 1.268)
0.394
Mother with obesity (yes vs. no)
0.170
1.185 (1.007, 1.394)
0.041
0.087
1.091 (0.788, 1.510)
0.602
0.185
1.203 (0.995, 1.454)
0.057
Physical activity
Weekday, min/day
−0.055
0.947 (0.899, 0.998)
0.040
−0.027
0.973 (0.879, 1.078)
0.603
−0.037
0.964 (0.906, 1.025)
0.235
Weekend, min/day
−0.023
0.977 (0.876, 1.091)
0.682
0.081
1.084 (0.879, 1.338)
0.450
−0.001
0.999 (0.877, 1.139)
0.993
Total, min/week
−0.096
0.908 (0.819, 1.008)
0.071
−0.023
0.977 (0.796, 1.200)
0.827
−0.058
0.944 (0.835, 1.067)
0.355
Dietary intake
Pickle, g
0.002
1.002 (1.001, 1.004)
<0.001
0.001
1.001 (0.997, 1.004)
0.963
0.002
1.002 (1.000, 1.003)
0.011
aAdjusted age, gender, and height. The values in bold indicate that the variables have significant correlation with childhood hypertension only in one region, either urban or rural.


### 3.4. Effects of SES, Perinatal, Anthropometric, Biochemical, and Blood Cell Composition Measures on BP

A multivariable logistic regression model was used to analyze the relationship of SES, perinatal, anthropometric, glycolipid, and blood cell composition indexes with childhood hypertension (in Table4). In the SES model, we found that income and medical insurance had a significant impact on BP (P<0.05), explaining 0.58%, 1.13% and 0.57% of the variance of childhood hypertension in total, urban and rural areas, respectively.Table 4
Model of SES, perinatal, anthropometric, serum biochemical and blood cell composition for childhood hypertension.
Variables
Total
Urban
Rural
OR (95% CI)
P
R2
OR (95% CI)
P
R2
OR (95% CI)
P
R2
SES model
a
0.58
1.13
0.57
Education, ref. ≤9y
∼12
0.983 (0.866, 1.115)
0.787
1.039 (0.800, 1.348)
0.776
1.137 (0.981, 1.317)
0.088
∼15
0.981 (0.794, 1.212)
0.860
1.096 (0.757, 1.588)
0.627
1.087 (0.829, 1.426)
0.544
>15
1.498 (0.824, 2.724)
0.185
1.755 (0.777, 3.968)
0.176
1.466 (0.583, 3.682)
0.416
Father’s occupation, ref. worker
Manager
1.001 (0.754, 1.327)
0.999
1.574 (0.941, 2.633)
0.084
0.753 (0.533, 1.064)
0.108
Technicist/researcher
1.103 (0.922, 1.319)
0.284
1.095 (0.744, 1.611)
0.646
1.088 (0.886, 1.335)
0.420
Farmer
1.080 (0.789, 1.477)
0.631
1.996 (1.226, 3.248)
0.005
0.719 (0.451, 1.148)
0.167
Others
0.952 (0.768, 1.179)
0.650
1.268 (0.835, 1.927)
0.265
0.894 (0.692, 1.155)
0.391
Mother’s occupation, ref. worker
Manager
1.097 (0.806, 1.492)
0.557
1.062 (0.623, 1.809)
0.826
1.033 (0.704, 1.514)
0.869
Technicist/researcher
1.062 (0.893, 1.262)
0.498
1.093 (0.795, 1.502)
0.586
1.055 (0.856, 1.302)
0.614
Farmer
1.246 (0.825, 1.880)
0.296
1.147 (0.608, 2.163)
0.672
1.296 (0.748, 2.247)
0.355
Others
1.022 (0.840, 1.244)
0.825
1.110 (0.751, 1.641)
0.600
0.966 (0.765, 1.219)
0.770
Income, ref. ≤500 RMB
∼1000
1.252 (0.995, 1.575)
0.055
1.146 (0.540, 2.430)
0.723
0.993(0.774,1.274)
0.957
∼2000
1.272 (1.063, 1.523)
0.009
1.021 (0.616, 1.691)
0.936
1.046(0.856,1.278)
0.660
∼3000
1.171 (1.007, 1.362)
0.041
0.948 (0.677, 1.327)
0.755
1.036(0.868,1.235)
0.696
>3000
1.123 (0.974, 1.294)
0.111
0.991 (0.761, 1.291)
0.948
1.059 (0.891, 1.260)
0.515
Living with grandparents (yes vs. no)
1.097 (0.949, 1.267)
0.211
0.777 (0.544, 1.109)
0.165
1.119 (0.952, 1.315)
0.172
People live with child, ref. 2∼3
1
1.094 (0.855, 1.399)
0.475
1.014 (0.550, 1.868)
0.965
1.028 (0.783, 1.348)
0.845
4
1.107 (0.992, 1.235)
0.068
1.103 (0.888, 1.370)
0.377
1.054 (0.927, 1.198)
0.424
Medical insurance (yes vs. no)
1.244 (1.080, 1.433)
0.002
0.882 (0.589, 1.320)
0.541
1.147 (0.983, 1.338)
0.081
Perinatal measure model
b
0.48
0.64
0.28
Gestational hypertension (yes vs. no)
1.475 (1.008, 2.160)
0.045
2.210 (1.102, 4.432)
0.025
1.209 (0.766, 1.907)
0.414
Birth weight, ref. 3000∼3600 g
∼3000
1.142 (1.005, 1.298)
0.041
1.057 (0.825, 1.355)
0.661
1.122 (0.965, 1.304)
0.134
>3600
0.923 (0.810, 1.052)
0.231
0.796 (0.615, 1.031)
0.084
0.948 (0.813, 1.104)
0.490
Breastfeeding, ref. 4∼10 months
0∼3
1.016 (0.887, 1.164)
0.820
1.253 (0.993, 1.582)
0.057
1.017 (0.857, 1.206)
0.851
>10
1.265 (1.118, 1.433)
<0.001
1.119 (0.840, 1.489)
0.442
1.149 (0.999, 1.323)
0.052
Mother with obesity (yes vs. no)
1.140 (0.962, 1.350)
0.130
1.040 (0.742, 1.458)
0.820
1.187 (0.974, 1.447)
0.089
Anthropometric measure model
c
10.89
11.09
11.73
Weight, kg
1.040 (1.035, 1.044)
<0.001
1.046 (1.038, 1.055)
<0.001
1.038 (1.033, 1.043)
<0.001
Height, cm
0.940 (0.932, 0.948)
<0.001
0.937 (0.921, 0.952)
<0.001
0.938 (0.929, 0.947)
<0.001
Heart rate, n/min
1.089 (1.079, 1.099)
<0.001
1.087 (1.068, 1.106)
<0.001
1.096 (1.084, 1.108)
<0.001
Total PA, ref. <150 min/week
0.902 (0.810, 1.005)
0.063
1.001 (0.810, 1.236)
0.998
0.925 (0.814, 1.051)
0.230
Serum biochemical index model
d
8.86
1.19
4.09
FBG, mmol/L
1.771 (1.560, 2.012)
<0.001
1.256 (1.037, 1.521)
0.020
1.103 (0.840, 1.447)
0.480
TC, mmol/L
0.803 (0.578, 1.114)
0.189
1.123 (0.775, 1.627)
0.539
1.318(0.684,2.537)
0.409
TG, mmol/L
1.326 (1.166, 1.507)
<0.001
1.304 (1.114, 1.527)
0.001
1.504 (1.187, 1.905)
0.001
HDL-C, mmol/L
1.114 (0.725, 1.711)
0.623
0.844 (0.502, 1.419)
0.523
0.420 (0.179, 0.986)
0.046
LDL-C, mmol/L
2.262 (1.629, 3.140)
<0.001
1.124 (0.693, 1.823)
0.636
0.940 (0.507, 1.744)
0.845
Blood cell composition model
e
12.04
3.76
7.76
RBC,n × 10^12/L
3.000 (1.716, 5.243)
<0.001
1.692 (0.790, 3.622)
0.176
2.657 (1.240, 5.692)
0.012
HGB, g/L
1.012 (0.989, 1.035)
0.319
1.025 (0.958, 1.097)
0.468
1.007 (0.986, 1.029)
0.508
HCT, %
1.051 (0.964, 1.147)
0.260
1.021 (0.812, 1.283)
0.862
1.017 (0.931, 1.112)
0.702
MCV, fL
1.007 (0.980, 1.035)
0.611
0.998 (0.967, 1.030)
0.909
1.021 (0.976, 1.067)
0.370
MCHC, g/L
1.010 (0.997, 1.023)
0.117
1.003 (0.976, 1.031)
0.834
0.999 (0.978, 1.019)
0.888
PLT,n×10^9/L
1.004 (1.002, 1.005)
<0.001
1.002 (0.999, 1.004)
0.056
1.004 (1.001, 1.006)
0.004
WSCR, %
0.997 (0.971, 1.024)
0.846
1.014 (0.971, 1.059)
0.523
0.988 (0.954, 1.022)
0.476
WMCR, %
1.016 (0.951, 1.086)
0.638
1.046 (0.966, 1.133)
0.269
1.060 (0.911, 1.233)
0.450
WSCC,n×10^9/L
1.261 (0.993, 1.601)
0.057
1.153 (0.784, 1.698)
0.469
1.249 (0.917, 1.702)
0.159
WMCC,n×10^9/L
0.588 (0.259, 1.337)
0.205
0.572 (0.208, 1.570)
0.278
0.392 (0.067, 2.301)
0.300
WLCC,n×10^9/L
1.057 (0.923, 1.209)
0.424
1.173 (0.965, 1.424)
0.108
1.073 (0.874, 1.317)
0.503
RDWSD, %
1.056 (1.018, 1.096)
0.003
0.976 (0.924, 1.031)
0.388
1.015 (0.963, 1.069)
0.583
MPV, fL
1.201 (1.111, 1.298)
<0.001
1.028 (0.922, 1.147)
0.618
1.140 (0.992, 1.308)
0.064
SES, socioeconomic status; PA, physical activity; FBG, fasting blood glucose; TC, total cholesterol; TG, triglyceride; HDL-C, high-density lipoprotein cholesterol; LDL-C, low-density lipoprotein cholesterol; WBC, white blood cell; RBC, red blood cell; HGB, hemoglobin; HCT, hematocrit; MCV, mean corpuscular volume; MCHC, mean corpuscular hemoglobin concentration; PLT, platelet; WSCR, WBC small cell ratio; WMCR, WBC middle cell ratio or mixed cell percent; WSCC, WBC small cell count or lymphocyte count; WMCC, WBC middle cell count or mixed cell count; WLCC, WBC large cell count or neutrophil count; RDWSD, red blood cell distribution width; MPV, mean platelet volume.aA total of 11265, 4294, 6971 subjects being included in SES model 1 of total, urban and rural, respectively. bA total of 11171, 4328, and 6843 subjects being included in SES model 1 of total, urban, and rural areas, respectively. cA total of 11795, 4491, and 7304 subjects being included in SES model 1 of total, urban, and rural areas, respectively. dA total of 5955,4743,1212 subjects being included in SES model 1 of total, urban, and rural areas, respectively. eA total of 5662, 4521, and 1141 subjects being included in SES model 1 of total, urban, and rural areas, respectively.In the perinatal measurement model, maternal gestational hypertension was a risk factor of childhood hypertension in urban regions but not in rural regions, while >10 months of breastfeeding was a predictor of hypertension (P<0.001), especially for children in rural areas (P=0.052). The perinatal measurement model explained 0.64% and 0.28% of the variance of childhood hypertension in urban and rural areas, respectively.Weight and heart rate were risk factors of elevated BP, whereas height was a protective factor against increased BP in both regions in the anthropometric measure model. Overall, the variance of BP explained in this model was almost equal between urban and rural areas (11.09% vs 11.73%).In the serum glycolipid index model, FBG was a risk factor for BP in urban regions (P=0.02) but not in rural regions, while HDL-C was a protective factor of hypertension only in rural regions (P<0.05). In addition, TG was an important impact factor of hypertension both in urban and rural areas (all P=0.001). This model explained a total of 1.19% and 4.09% of the variance of BP in urban and rural regions, respectively.In the blood-cell composition model, RBC, PLT, RDWSD, and MPV were risk factors for high BP in total population, and RBC and PLT impact the BP level of children living in rural areas (P<0.05). The variance of BP explained in the blood-cell composition model in rural areas was higher than that in urban areas (7.76% vs 3.76%).


### 3.5. Full Model to Analyze the Risk Factors of BP

The significant variables among the SES, perinatal, anthropometric, biochemical, and blood cell composition measure models and dietary variables were included in full model 1(in Table5). Children living in rural areas have the increased odds ratio of hypertension compared with those in urban areas (2.21(1.93, 2.53), P<0.001). Pickle intake and low birth weight (<3000 g) were significantly and positively associated with hypertension only in rural regions, but maternal gestational hypertension had significant increasing effects on the BP level only in urban regions. In addition, increased BMI and heart rate were risk factors for childhood hypertension both in urban and rural regions. Full model 1 could explain 13.61%, 10.88%, and 12.23% of the variance of childhood hypertension in total, urban, and rural areas, respectively.Table 5
Full model of risk factors for childhood hypertension.
Variables
Total
Urban
Rural
OR (95% CI)
P
R2
OR (95% CI)
P
R2
OR (95% CI)
P
R2 (%)
Full model 1
a
13.61
10.88
12.23
Region (urban vs. rural)
2.208 (1.926, 2.531)
<0.001
Income, ref. ≤500 RMB
∼1000
1.052 (0.804, 1.377)
0.710
1.128 (0.424, 3.001)
0.809
1.037 (0.783, 1.373)
0.799
∼2000
1.035 (0.804, 1.332)
0.790
1.029 (0.416, 2.542)
0.951
1.040 (0.798, 1.356)
0.771
∼3000
1.004 (0.781, 1.290)
0.974
1.024 (0.422, 2.482)
0.958
1.004 (0.769, 1.310)
0.976
>3000
0.950 (0.744, 1.213)
0.682
1.016 (0.426, 2.420)
0.972
0.920 (0.709, 1.194)
0.532
Medical insurance (yes vs. no)
0.901 (0.774, 1.048)
0.177
1.173 (0.771, 1.784)
0.457
0.861 (0.730, 1.015)
0.075
Total PA, ref. <150 min/week
0.929 (0.829, 1.041)
0.202
0.927 (0.746, 1.153)
0.497
0.920 (0.805, 1.053)
0.226
Pickle, g
1.002 (1.001, 1.003)
0.025
1.001 (0.996, 1.004)
0.877
1.002 (1.001, 1.004)
0.010
Perinatal measures
Gestational hypertension (yes vs. no)
1.615 (1.083, 2.407)
0.019
2.461 (1.203, 5.036)
0.014
1.380 (0.857, 2.222)
0.186
Birth weight, ref. 3000∼3600 g
∼3000
1.152 (1.005, 1.321)
0.042
1.060 (0.814, 1.381)
0.664
1.188 (1.013, 1.394)
0.035
>3600
0.932 (0.812, 1.070)
0.315
0.839 (0.642, 1.097)
0.201
0.966 (0.821, 1.135)
0.671
Breastfeeding, ref. 4∼10 months
0∼3
1.067 (0.923, 1.232)
0.382
1.206 (0.945, 1.538)
0.133
0.991 (0.827, 1.187)
0.918
>10
1.124 (0.984, 1.284)
0.085
1.043 (0.770, 1.413)
0.786
1.134 (0.977, 1.317)
0.097
Anthropometric measures
BMI
1.184 (1.161, 1.208)
<0.001
1.151 (1.109, 1.194)
<0.001
1.199 (1.171, 1.227)
<0.001
Height, cm
0.992 (0.983, 1.002)
0.107
0.994 (0.976, 1.012)
0.514
0.992 (0.981, 1.003)
0.148
Heart rate, n/min
1.040 (1.036, 1.045)
<0.001
1.047 (1.038, 1.056)
<0.001
1.037 (1.032, 1.043)
<0.001
Full model 2
b
23.25
11.12
25.04
Region (urban vs. rural)
2.310 (1.667, 3.205)
<0.001
Income, ref. ≤500 RMB
∼1000
1.610 (0.898, 2.886)
0.328
1.021 (0.314, 3.325)
0.970
1.723 (0.873, 3.401)
0.374
∼2000
1.544 (0.896, 2.662)
0.385
1.077 (0.362, 3.209)
0.814
1.619 (0.848, 3.090)
0.537
∼3000
1.574 (0.921, 2.691)
0.261
1.105 (0.378, 3.233)
0.676
1.658(0.876,3.136)
0.423
>3000
1.373 (0.811, 2.322)
0.836
0.960(0.334,2.760)
0.625
1.492 (0.799, 2.789)
0.921
Medical insurance (yes vs. no)
0.841 (0.636, 1.112)
0.224
1.009 (0.632,1.613)
0.969
0.734 (0.509, 1.059)
0.098
Total PA, ref. <150 min/week
0.989 (0.814, 1.200)
0.908
0.907(0.703, 1.170)
0.451
1.102 (0.811, 1.498)
0.534
Pickle, g/day
1.114 (0.965, 1.287)
0.140
1.038 (0.830, 1.298)
0.742
1.177 (0.965, 1.435)
0.108
Perinatal measures
Gestational hypertension (yes vs. no)
1.939 (0.966, 3.891)
0.062
2.535 (1.117, 5.753)
0.026
1.055 (0.326, 3.421)
0.929
Birth weight, ref. 3000∼3600 g
∼3000
1.008 (0.794, 1.280)
0.759
1.057 (0.777, 1.437)
0.425
0.960 (0.657, 1.405)
0.607
>3600
0.948 (0.753, 1.195)
0.604
0.880 (0.644, 1.203)
0.303
1.106 (0.772, 1.583)
0.469
Breastfeeding, ref. 4∼10 months
0∼3
1.255 (0.993, 1.586)
0.416
1.144 (0.857, 1.526)
0.615
1.436 (0.951, 2.167)
0.425
>10
1.312 (1.035, 1.663)
0.166
1.133 (0.806, 1.595)
0.723
1.514 (1.075, 2.134)
0.156
Anthropometric measures
BMI, kg/m2
1.146 (1.112, 1.181)
<0.001
1.155 (1.102, 1.211)
<0.001
1.138 (1.092, 1.185)
<0.001
Height, cm
0.987 (0.978, 0.997)
0.009
0.975 (0.962, 0.988)
<0.001
1.006 (0.991, 1.021)
0.448
Heart rate, n/min
1.046 (1.039, 1.054)
<0.001
1.043 (1.033, 1.054)
<0.001
1.051 (1.038, 1.064)
<0.001
Biochemical index measures
FBG, mmol/L
1.080 (0.890, 1.311)
0.434
1.048 (0.820, 1.341)
0.706
1.207 (0.862, 1.689)
0.273
TC, mmol/L
0.940 (0.781, 1.132)
0.516
0.966 (0.753, 1.239)
0.785
0.859 (0.647, 1.141)
0.294
HDL-C, mmol/L
1.196 (0.783, 1.828)
0.408
1.185 (0.670, 2.096)
0.560
1.157 (0.606, 2.210)
0.658
RBC,n×10^12/L
1.946 (1.409, 2.687)
<0.001
1.742 (1.139, 2.664)
0.010
2.095 (1.239, 3.544)
0.006
HGB, g/L
1.006 (0.994, 1.018)
0.348
1.010 (0.992, 1.028)
0.274
0.999 (0.983, 1.016)
0.942
WSCR, %
1.005 (0.994, 1.017)
0.366
1.012 (0.996, 1.028)
0.128
0.996 (0.978, 1.014)
0.668
MPV, fL
1.017 (0.939, 1.101)
0.679
1.043 (0.945, 1.151)
0.408
0.954 (0.803, 1.096)
0.502
PA, physical activity; BMI, body mass index; FBG, fasting blood glucose; TC, total cholesterol; HDL-C, high-density lipoprotein cholesterol; RBC, red blood cell; HGB, hemoglobin; WSCR, white blood cell small cell ratio; MPV, mean platelet volume.aA total of 11014, 4265, and 6749 subjects being included in full model 1 of total, urban, and rural areas, respectively, and adjusted age and gender. bA total of 4265, 3257, and 1008 subjects being included in full model 2 of total, urban, and rural areas, respectively, and adjusted age and gender. The values in bold indicate that the variables have significant correlation with childhood hypertension.Glycolipid index and blood cell compositions were added in full model 2 (in Table5). In full model 2, rural residence, BMI, height, heart rate, and RBC were significant impact factors of childhood hypertension. In addition, maternal gestational hypertension was positively associated with hypertension, while height was negatively associated with BP in urban children (all P<0.05). Moreover, BMI, heart rate, and RBC were positively associated with hypertension both in urban and rural areas (all P<0.05). This model explained 23.25%, 11.12%, and 25.04% of the variance of hypertension in total, urban, and rural areas, respectively.


## 3.1. General Characteristics

The general characteristics of the subjects are presented in Table1. The mean age was 9.23 ± 1.76 years, and 51.92% (7060/13597) were males. Compared with children from urban areas, children living in rural areas had significantly elevated age, heart rate, FGB, TC, TG, HDL-C, LDL-C, white blood cell (WBC), red blood cell (RBC), hemoglobin (HGB), hematocrit (HCT), mean corpuscular volume (MCV), WBC small cell ratio (WSCR), WBC small cell count or lymphocyte count (WSCC), red blood cell distribution width (RDWSD), mean platelet volume (MPV), and the percent of living with grandparents, but significantly reduced BMI, weight, waist circumference, MCHC, PLT, WMCR, WMCC, WLCC, PA, and father with obesity (all P<0.05, Table 1). There was significant difference in the percent of father’s education, parental occupation, income, people live with child, birth weight, and breastfeeding between the urban and rural groups. In addition, in Supplementary Table 1, the average intake amount of fruit, fish, eggs, milk, bean food, nuts, mushrooms and algae food, and vegetables was lower for children living in rural areas than those living in urban areas (all P<0.05), and the average intake of cereals and potatoes, pickle, and nutritional supplements was higher for children living in rural areas than its counterparts (P<0.05).Table 1
General characteristics of children between urban and rural areas.
Variables
Total
Urban vs rural
Urban (n = 5995)
Rural (n = 7602)
P
Anthropometric measures
Gender, male (n (%))
7060 (51.92%)
3098 (51.68%)
3962 (52.12%)
0.609
Age, y
9.24 ± 1.76
9.05 ± 1.76
9.39 ± 1.74
<0.001
BMI, kg/m2
17.35 ± 3.05
17.53 ± 3.02
17.22 ± 3.07
<0.001
Height, cm
134.51 ± 11.5
134.66 ± 11.44
134.39 ± 11.56
0.182
Weight, kg
32.01 ± 9.49
32.40 ± 9.53
31.7 ± 9.45
<0.001
Waist circumference, cm
58.12 ± 8.49
59.17 ± 8.73
57.3 ± 8.21
<0.001
Heart rate, n/min
95.28 ± 12.72
94.95 ± 12.71
95.54 ± 12.72
0.007
Serum biochemical index
FBG, mmol/L
4.14 ± 0.62
3.94 ± 0.50
4.90 ± 0.46
<0.001
TC, mmol/L
3.49 ± 0.66
3.34 ± 0.57
4.10 ± 0.63
<0.001
TG, mmol/L
0.97 ± 0.58
0.96 ± 0.58
1.03 ± 0.57
<0.001
HDL-C, mmol/L
1.22 ± 0.27
1.19 ± 0.26
1.35 ± 0.28
<0.001
LDL-C, mmol/L
1.72 ± 0.54
1.56 ± 0.39
2.35 ± 0.60
<0.001
Blood cell composition measures
WBC,n × 10∧9/L
7.12 ± 1.79
7.12 ± 1.77
7.16 ± 1.85
0.480
RBC,n × 10∧12/L
4.59 ± 0.39
4.52 ± 0.35
4.90 ± 0.40
<0.001
HGB, g/L
126.42 ± 9.98
124.31 ± 8.56
134.73 ± 10.82
<0.001
HCT, %
37.66 ± 3.11
36.74 ± 2.43
41.29 ± 2.78
<0.001
MCV, fL
81.91 ± 6.44
81.37 ± 5.98
84.01 ± 7.66
<0.001
MCH, pg
27.65 ± 1.95
27.64 ± 1.94
27.69 ± 1.96
0.390
MCHC, g/L
336.58 ± 14.98
338.99 ± 15.08
327.11 ± 10.06
<0.001
PLT,n × 10∧9/L
287.48 ± 67.49
288.53 ± 67.42
283.35 ± 67.63
0.020
WSCR, %
36.11 ± 8.25
35.59 ± 8.03
38.15 ± 8.80
<0.001
WMCR, %
7.05 ± 2.75
7.41 ± 2.87
5.64 ± 1.60
<0.001
WSCC,n × 10∧9/L
2.52 ± 0.66
2.47 ± 0.63
2.70 ± 0.73
<0.001
WMCC,n × 10∧9/L
0.51 ± 0.34
0.53 ± 0.28
0.44 ± 0.48
<0.001
WLCC,n × 10∧9/L
4.08 ± 1.49
4.12 ± 1.48
3.90 ± 1.50
<0.001
RDWSD, %
34.59 ± 4.82
33.40 ± 4.23
39.27 ± 4.09
<0.001
PDW, fL
13.47 ± 2.37
13.48 ± 2.32
13.39 ± 2.57
0.207
MPV, fL
10.68 ± 1.30
10.57 ± 1.32
11.11 ± 1.15
<0.001
P-LCR, %
32.67 ± 8.65
32.66 ± 8.86
32.69 ± 7.76
0.910
Socioeconomic index
Father’s education level, y
∼9
6856 (51.75%)
2190 (37.80%)
4666 (62.58%)
<0.001
∼12
4915 (37.10%)
2787 (48.11%)
2128 (28.54%)
∼15
1390 (10.49%)
755 (13.03%)
635 (8.52%)
>15
88 (0.66%)
61 (1.05%)
27 (0.36%)
Father’s occupation
Manager
933 (7.02%)
389 (6.70%)
544 (7.26%)
<0.001
Worker
4016 (30.21%)
1733 (29.84%)
2283 (30.49%)
Technicist/researcher
571 (4.29%)
380 (6.54%)
191 (2.55%)
Farmer
4593 (34.55%)
1686 (29.03%)
2907 (38.82%)
Others
3182 (23.93%)
1619 (27.88%)
1563 (20.87%)
Mother’s occupation
Manager
605 (4.55%)
241 (4.12%)
364 (4.88%)
<0.001
Worker
3538 (26.58%)
1666 (28.45%)
1872 (25.11%)
Technicist/researcher
239 (1.80%)
132 (2.25%)
107 (1.44%)
Farmer
4179 (31.40%)
2111 (36.05%)
2068 (27.74%)
Others
4750 (35.68%)
1705 (29.12%)
3045 (40.84%)
Income, RMB
∼500
738 (6.25%)
92 (2.05%)
646 (8.83%)
<0.001
∼1000
1395 (11.82%)
235 (5.23%)
1160 (15.86%)
∼2000
2327 (19.72%)
627 (13.96%)
1700 (23.25%)
∼3000
2750 (23.30%)
1051 (23.41%)
1699 (23.24%)
>3000
4592 (38.91%)
2485 (55.35%)
2107 (28.82%)
Living with grandparents
No
10060 (84.76%)
3992 (88.42%)
6068 (82.51%)
<0.001
Yes
1809 (15.24%)
523 (11.58%)
1286 (17.49%)
People live with child
1
583 (4.98%)
154 (3.43%)
429 (5.94%)
<0.001
2∼3
6114 (52.22%)
2595 (57.81%)
3519 (48.74%)
4
5012 (42.80%)
1740 (38.76%)
3272 (45.32%)
Medical insurance
No
1790 (15.14%)
383 (8.50%)
1407 (19.24%)
<0.001
Yes
10030 (84.86%)
4124 (91.50%)
5906 (80.76%)
Perinatal measures
Gestational hypertension
No
11299 (98.47%)
4366 (98.76%)
6933 (98.30%)
0.052
Yes
175 (1.53%)
55 (1.24%)
120 (1.70%)
Birth weight, g
∼3000
3181 (26.86%)
1133 (25.16%)
2048 (27.91%)
<0.001
∼3600
5051 (42.65%)
2058 (45.70%)
2993 (40.78%)
>3600
3610 (30.48%)
1312 (29.14%)
2298 (31.31%)
Breastfeeding, month
0∼3
2845 (24.40%)
1420 (31.77%)
1425 (19.81%)
<0.001
4∼10
5555 (47.64%)
2223 (49.74%)
3332 (46.33%)
>10
3261 (27.97%)
826 (18.48%)
2435 (33.86%)
Father with obesity
No
9839 (83.89%)
3703 (82.79%)
6136 (84.56%)
0.011
Yes
1890 (16.11%)
770 (17.21%)
1120 (15.44%)
Mother with obesity
No
10505 (89.57%)
4017 (89.73%)
6488 (89.48%)
0.670
Yes
1223 (10.43%)
460 (10.27%)
763 (10.52%)
Physical activity
Weekday, min/day
∼10
6321 (53.41%)
2246 (49.87%)
4075 (55.59%)
<0.001
>10
5514 (46.59%)
2258 (50.13%)
3256 (44.41%)
Weekend, min/day
∼55
7782 (65.77%)
2795 (62.11%)
4987 (68.01%)
<0.001
>55
4051 (34.23%)
1705 (37.89%)
2346 (31.99%)
Total, min/week
∼150
6210 (52.59%)
2170 (48.32%)
4040 (55.21%)
<0.001
>150
5599 (47.41%)
2321 (51.68%)
3278 (44.79%)
BMI, body mass index; FBG, fasting blood glucose; TC, total cholesterol; TG, triglyceride; HDL-C, high-density lipoprotein cholesterol; LDL-C, low-density lipoprotein cholesterol; WBC, white blood cell; RBC, red blood cell; HGB, hemoglobin; HCT, hematocrit; MCV, mean corpuscular volume; MCH, mean corpuscular hemoglobin; MCHC, mean corpuscular hemoglobin concentration; PLT, platelet; WSCR, WBC small cell ratio; WMCR, WBC middle cell ratio or mixed cell percent; WSCC, WBC small cell count or lymphocyte count; WMCC, WBC middle cell count or mixed cell count; WLCC, WBC large cell count or neutrophil count; RDWSD, red blood cell distribution width; PDW, platelet distribution width; MPV, mean platelet volume; P-LCR, platelet-large cell ratio.


## 3.2. Prevalence of Childhood Hypertension

Overall, the prevalence of hypertension in 6∼12-year-old children was 13.75%, and there was significant difference between urban and rural areas (8.44% (7.32%, 9.56%),P<0.001) (Table 2). The prevalence was elevated in children with the characteristics of living in rural areas, younger age group, obesity, low family income, living with grandparents, no medical insurance, maternal gestational hypertension, low birth weight (LBW), >10 months of breastfeeding, parental obesity, and low PA (all P<0.05) (Table 2). In the subgroup analyses by areas, the prevalence of hypertension in children with maternal gestational hypertension was markedly elevated in urban areas (P<0.05) but not in rural areas (Table 2). The prevalence was higher for children with the characteristics of younger age group and overweight/obesity, in both urban and rural areas. In the subgroup analyses of age, obesity, income, living with grandparents, people live with child, medical insurance, birth weight, breast feeding, father with obesity, mother with obesity, and PA, the prevalence of hypertension in rural children was higher than those of urban children (all P<0.05) (Table 2).Table 2
Prevalence of hypertension of children aged 6–12 years.
Variables
Total
Urban
Rural
Difference (rural vs urban)
P
Prevalence
P
Prevalence
P
Prevalence
P
Total
13.75% (13.17%, 14.34%)
9.02% (8.31%, 9.78%)
17.47% (16.62%, 18.34%)
8.44% (7.32%, 9.56%)
<0.001
Anthropometric measures
Gender
Male
13.50% (12.71%, 14.32%)
0.385
8.72% (7.75%, 9.76%)
0.388
17.24% (16.07%, 18.45%)
0.581
8.52% (6.98%, 10.06%)
<0.001
Female
14.01% (13.18%, 14.88%)
9.35% (8.32%, 10.47%)
17.72% (16.49%, 19.00%)
8.37% (6.73%, 10.00%)
<0.001
Age, y
6
16.73% (14.92%, 18.66%)
<0.001
14.15% (11.83%, 16.72%)
<0.001
19.50% (16.75%, 22.49%)
<0.001
5.36% (1.67%, 9.04%)
0.004
7
15.66% (14.26%, 17.15%)
10.91% (9.23%, 12.79%)
20.35% (18.15%, 22.69%)
9.44% (6.61%, 12.26%)
<0.001
8
13.70% (12.32%, 15.17%)
7.30% (5.79%, 9.05%)
18.97% (16.85%, 21.24%)
11.67% (8.99%, 14.35%)
<0.001
9
11.68% (10.34%, 13.13%)
8.16% (6.47%, 10.12%)
14.41% (12.46%, 16.53%)
6.25% (3.58%, 8.91%)
<0.001
10
12.25% (10.90%, 13.70%)
7.31% (5.65%, 9.28%)
15.39% (13.49%, 17.44%)
8.08% (5.47%, 10.69%)
<0.001
11
13.28% (11.89%, 14.77%)
6.37% (4.84%,8.22%)
17.72% (15.71%, 19.87%)
11.35%(8.74%,13.96%)
<0.001
12
12.69% (10.35%, 15.33%)
8.24% (5.24%, 12.21%)
15.28% (12.11%, 18.91%)
7.04% (2.38%, 11.71%)
0.003
Obesity
Normal
11.33% (10.70%, 11.99%)
<0.001
7.01% (6.24%, 7.84%)
<0.001
14.59% (13.65%, 15.56%)
<0.001
7.57% (6.35%, 8.80%)
<0.001
Overweight
15.50% (14.24%, 16.83%)
10.16% (8.63%, 11.85%)
20.04%(18.14%,22.04%)
9.88%(7.39%,12.37%)
<0.001
Obesity
29.40% (26.73%, 32.17%)
21.23% (17.84%, 24.93%)
37.06% (33.09%, 41.17%)
15.83% (10.58%, 21.09%)
<0.001
Socioeconomic index
Father’s education level, ya
∼9
14.22% (13.40%, 15.07%)
0.256
8.36% (7.23%, 9.59%)
0.098
16.97% (15.91%, 18.08%)
0.465
8.62% (7.04%, 10.20%)
<0.001
∼12
13.06% (12.13%, 14.04%)
8.97% (7.93%, 10.09%)
18.42% (16.79%, 20.13%)
9.45% (7.49%, 11.41%)
<0.001
∼15
13.81% (12.04%, 15.74%)
10.73% (8.61%, 13.16%)
17.48% (14.60%, 20.66%)
6.75% (3.06%, 10.44%)
<0.001
>15
17.05% (9.87%, 26.55%)
14.75% (6.98%, 26.17%)
22.22% (8.62%, 42.26%)
7.47% (−10.56%, 25.5%)
0.417
Father’s occupationb
Manager
13.29% (11.18%, 15.64%)
0.110
11.31% (8.34%, 14.89%)
0.007
14.71% (11.84%, 17.97%)
0.006
3.39% (−0.94%, 7.73%)
0.125
Worker
14.82% (13.73%, 15.95%)
8.25% (7.00%, 9.65%)
19.80% (18.18%, 21.49%)
11.55% (9.46%, 13.63%)
<0.001
Technicist/researcher
13.84% (11.11%, 16.94%)
13.42% (10.16%, 17.27%)
14.66% (9.97%, 20.49%)
1.24% (−4.84%, 7.31%)
0.689
Farmer
13.89% (12.90%, 14.92%)
8.24% (6.98%, 9.66%)
17.17% (15.81%, 18.59%)
8.92% (7.02%, 10.82%)
<0.001
Others
12.60% (11.47%, 13.81%)
9.20% (7.84%, 10.72%)
16.12% (14.33%, 18.04%)
6.92% (4.62%, 9.22%)
<0.001
Mother’s occupationc
Manager
13.76% (11.11%, 16.77%)
0.096
10.79% (7.17%, 15.41%)
0.104
15.75% (12.15%, 19.91%)
0.268
4.87% (−0.54%, 10.28%)
0.078
Worker
14.32% (13.18%, 15.52%)
8.84% (7.51%, 10.31%)
19.16% (17.40%, 21.01%)
10.24% (8.00%, 12.49%)
<0.001
Technicist/researcher
16.74% (12.23%, 22.09%)
15.15% (9.51%, 22.43%)
18.69% (11.81%, 27.38%)
3.54% (−6.05%, 13.13%)
0.469
Farmer
12.74% (11.74%, 13.79%)
8.79% (7.61%, 10.09%)
16.73% (15.15%, 18.41%)
8.06% (6.06%, 10.07%)
<0.001
Others
14.26% (13.27%, 15.29%)
8.62% (7.33%, 10.06%)
17.40% (16.06%, 18.79%)
8.78% (6.89%, 10.68%)
<0.001
Income, RMBd
∼500
16.26% (13.67%, 19.12%)
0.009
9.78% (4.57%, 17.76%)
0.880
17.18% (14.35%, 20.32%)
0.988
7.40% (0.67%, 14.13%)
0.031
∼1000
16.06% (14.17%, 18.09%)
8.51% (5.28%, 12.84%)
17.59% (15.44%, 19.90%)
9.08% (4.89%, 13.26%)
<0.001
∼2000
15.13% (13.69%, 16.65%)
8.29% (6.26%, 10.73%)
17.65% (15.86%, 19.54%)
9.35% (6.54%, 12.17%)
<0.001
∼3000
14.33% (13.04%, 15.69%)
8.85% (7.20%, 10.73%)
17.72% (15.93%, 19.62%)
8.87% (6.37%, 11.37%)
<0.001
>3000
13.00% (12.04%, 14.01%)
9.50% (8.37%, 10.72%)
17.13% (15.55%, 18.81%)
7.64% (5.66%, 9.62%)
<0.001
Living with grandparentse
No
13.97% (13.29%, 14.66%)
0.039
9.27% (8.39%, 10.21%)
0.355
17.06% (16.12%, 18.03%)
0.099
7.79% (6.48%, 9.09%)
<0.001
Yes
15.81% (14.16%, 17.57%)
8.03% (5.85%, 10.70%)
18.97% (16.86%, 21.23%)
10.94% (7.78%, 14.11%)
<0.001
People live with childf
1
15.44% (12.60%, 18.63%)
0.106
9.09% (5.06%, 14.78%)
0.687
17.72% (14.22%, 21.66%)
0.802
8.62% (2.82%, 14.43%)
0.004
2∼3
13.58% (12.73%, 14.46%)
8.82% (7.76%, 9.98%)
17.08% (15.85%, 18.36%)
8.25% (6.60%, 9.91%)
<0.001
4
14.86% (13.89%, 15.88%)
9.60% (8.25%, 11.08%)
17.67% (16.37%, 19.02%)
8.07% (6.16%, 9.97%)
<0.001
Medical insuranceg
No
13.83% (13.16%, 14.52%)
0.004
9.26% (8.39%, 10.19%)
0.209
17.01% (16.06%, 17.99%)
0.096
11.59% (8.28%, 14.91%)
<0.001
Yes
16.41% (14.72%, 18.20%)
7.31% (4.91%, 10.39%)
18.88% (16.87%, 21.02%)
7.79% (6.49%, 9.10%)
<0.001
Perinatal measures
Gestational hypertensionh
No
14.02% (13.38%, 14.67%)
0.024
8.96% (8.12%, 9.84%)
0.018
17.21% (16.33%, 18.12%)
0.297
8.25% (7.02%, 9.48%)
<0.001
Yes
20.00% (14.34%, 26.70%)
18.18% (9.08%, 30.90%)
20.83% (13.96%, 29.20%)
2.65% (−9.87%, 15.17%)
0.678
Birth weight, gi
∼3000
15.59% (14.35%, 16.90%)
0.026
9.89% (8.21%, 11.77%)
0.110
18.75% (17.08%, 20.51%)
0.149
8.86% (6.44%, 11.29%)
<0.001
∼3600
14.04% (13.09%, 15.03%)
9.52% (8.29%, 10.87%)
17.14% (15.81%, 18.54%)
7.62% (5.76%, 9.47%)
<0.001
>3600
13.35% (12.26%, 14.50%)
7.70% (6.31%, 9.28%)
16.58% (15.08%, 18.16%)
8.88% (6.79%, 10.98%)
<0.001
Breastfeeding, monthj
0∼3
13.74% (12.50%, 15.06%)
<0.001
10.42% (8.88%, 12.13%)
0.090
17.05% (15.13%, 19.11%)
0.084
6.63% (4.11%, 9.15%)
<0.001
4∼10
13.21% (12.33%, 14.13%)
8.28% (7.16%, 9.50%)
16.51% (15.26%, 17.81%)
8.23% (6.53%, 9.93%)
<0.001
>10
16.31% (15.06%, 17.63%)
9.20% (7.32%, 11.38%)
18.73% (17.20%, 20.33%)
9.53% (7.02%, 12.03%)
<0.001
Father with obesityk
No
14.02% (13.36%, 14.70%)
0.149
8.99% (8.12%, 9.91%)
0.126
17.14% (16.23%, 18.08%)
0.263
8.42% (7.10%, 9.73%)
<0.001
Yes
16.19% (14.17%, 18.38%)
10.00% (7.41%, 13.11%)
19.92% (17.14%, 22.93%)
8.05% (4.91%, 11.20%)
<0.001
Mother with obesityl
No
14.03% (13.35%, 14.73%)
0.040
8.78% (7.88%, 9.73%)
0.474
17.19% (16.26%, 18.16%)
0.055
8.15% (6.88%, 9.43%)
<0.001
Yes
15.29% (13.70%, 16.99%)
10.52% (8.44%, 12.90%)
18.57% (16.33%, 20.98%)
9.92% (5.98%, 13.86%)
<0.001
Physical activity
Weekday, min/daym
∼10
14.89% (14.02%, 15.79%)
0.028
9.26% (8.09%, 10.54%)
0.637
17.99% (16.82%, 19.20%)
0.141
8.73% (7.05%, 10.41%)
<0.001
>10
13.47% (12.58%, 14.40%)
8.86% (7.72%, 10.11%)
16.68% (15.41%, 18.00%)
7.82% (6.08%, 9.56%)
<0.001
Weekend, min/dayn
∼55
14.37% (13.59%, 15.17%)
0.560
8.91% (7.88%, 10.03%)
0.590
17.43% (16.38%, 18.51%)
0.900
8.52% (7.03%, 10.01%)
<0.001
>55
13.97% (12.92%, 15.08%)
9.38% (8.04%, 10.87%)
17.31% (15.80%, 18.90%)
7.92% (5.86%, 9.99%)
<0.001
Total, min/weeko
∼150
14.83% (13.96%, 15.74%)
0.050
9.17% (7.99%, 10.46%)
0.808
17.87% (16.70%, 19.09%)
0.247
8.70% (7.01%, 10.39%)
<0.001
>150
13.57% (12.69%, 14.50%)
8.96% (7.83%, 10.20%)
16.84% (15.57%, 18.17%)
7.88% (6.15%, 9.61%)
<0.001
aA total of 348 subjects having missing values in father’s education level; bA total of 302 subjects having missing values in father’s occupation. cA total of 286 subjects having missing values in mother’s occupation. dA total of 1795 subjects having missing values in parents’ income. eA total of 1728 subjects having missing values in living with grandparents. fA total of 1888 subjects having missing values in people live with child. gA total of 1777 subjects having missing values in medical insurance. hA total of 2123 subjects having missing values in gestational hypertension. iA total of 1755 subjects having missing values in birth weight. jA total of 1936 subjects having missing values in breastfeeding. kA total of 1868 subjects having missing values in father with obesity. lA total of 1869 subjects having missing values in mother with obesity. mA total of 1762 subjects having missing values in weekday activity. nA total of 1764 subjects having missing values in weekend activity. oA total of 1788 subjects having missing values in total activityThe prevalence of childhood hypertension was also analyzed in relation to pairs of the following variables: birth weight, duration of breastfeeding, family income, obesity, and living with grandparents. In Figure1 and Figure 2, combinations of the above risk factors led to significantly higher risks for HTN in both urban and rural areas, it is shown in Figures 1 and 2 and Supplementary Tables 2–5. In Figure 1(a), children with >10 months of breastfeeding duration and living with grandparents had a significantly higher prevalence of hypertension than other groups (16.98%, P<0.05) in urban regions but not in rural regions. In addition, obese children with 0∼3 months of breastfeeding duration or LBW (<3000 g) have the highest prevalence of hypertension in urban regions (25.19%, P<0.001; 22.37%, P<0.001) (Figures 1(c) and 2(c)), while obese children with >10 months of breastfeeding duration or LBW (<3000 g) have the highest prevalence of hypertension in rural regions (39.80%, P<0.001; 43.80%, P<0.001) (in Figure 1(d) and Figure 2(d)).Figure 1
Prevalence of hypertension for children having different durations of breastfeeding combined with other characteristics. (a). Prevalence of hypertension for children combined with duration of breastfeeding and living with grandparents in urban regions. (b). Prevalence of hypertension for children combined with duration of breastfeeding and living with grandparents in rural regions. (c). Prevalence of hypertension for children combined with duration of breastfeeding and obesity in urban regions. (d). Prevalence of hypertension for children combined with duration of breastfeeding and obesity in rural regions. (e). Prevalence of hypertension for children combined with duration of breastfeeding and birth weight in urban regions. (f) Prevalence of hypertension for children combined with duration of breastfeeding and birth weight in rural regions.
(a)
(b)
(c)
(d)
(e)
(f)Figure 2
Prevalence of hypertension for children combined with two characteristics. (a). Prevalence of hypertension for children combined with present weight and living with grandparents in urban regions. (b). Prevalence of hypertension for children combined with present weight and living with grandparents in rural regions. (c). Prevalence of hypertension for children combined with birth weight and present weight in urban regions. (d). Prevalence of hypertension for children combined with birth weight and present weight in rural regions. (e). Prevalence of hypertension for children combined with income and present weight in urban regions. (f). Prevalence of hypertension for children combined with income and present weight in rural regions.
(a)
(b)
(c)
(d)
(e)
(f)


## 3.3. Using Univariate Model to Analyze the Risk Factors of Childhood Hypertension

The results of the univariate logistic regression model, adjusting age, sex, and height, illustrated that overweight/obesity, maternal gestational hypertension, >10 months of breastfeeding, maternal obesity, less weekday PA, and more pickle intake were risk factors for childhood hypertensive (allP<0.05), whereas a paternal education level of 9∼12 years (ref. ≤ 9 y), high income, and increased birth weight were protective factors against childhood hypertensive (all P<0.05) (Table 3). In the subgroup analyses, maternal gestational hypertension was a predictor of hypertension for urban children, while >10 months of breastfeeding and pickle intake effect the BP level only in rural areas (P<0.05).Table 3
Results of the univariate logistic model of the risk factors for childhood hypertension.
Variablesa
Total
Urban
Rural
β
OR (95% CI)
P
β
OR (95% CI)
P
β
OR (95% CI)
P
Region (urban vs. rural)
−0.209
0.811 (0.707, 0.931)
0.003
Obesity, ref. normal
Overweight
0.404
1.497 (1.330, 1.686)
<0.001
0.446
1.563 (1.260, 1.938)
<0.001
0.395
1.484 (1.285, 1.714)
<0.001
Obesity
1.283
3.608 (3.095, 4.206)
<0.001
1.354
3.872 (2.988, 5.017)
<0.001
1.276
3.584 (2.946, 4.359)
<0.001
Education, ref. ≤9 y
∼12
−0.137
0.872 (0.782, 0.972)
0.014
−0.003
0.997 (0.815, 1.221)
0.981
0.068
1.070 (0.936, 1.225)
0.322
∼15
−0.087
0.917 (0.775, 1.085)
0.313
0.166
1.181 (0.893, 1.562)
0.244
−0.017
0.983 (0.789, 1.225)
0.877
>15
0.156
1.169 (0.667, 2.049)
0.585
0.523
1.687 (0.814, 3.497)
0.159
0.306
1.358 (0.545, 3.383)
0.511
Father’s occupation, ref. worker
Manager
−0.141
0.869 (0.705, 1.071)
0.187
0.326
1.386 (0.965, 1.990)
0.077
−0.384
0.681 (0.525, 0.883)
0.004
Technicist/researcher
−0.108
0.898 (0.697, 1.157)
0.404
0.498
1.646 (1.168, 2.318)
0.004
−0.377
0.686 (0.453, 1.039)
0.075
Farmer
−0.078
0.925 (0.819, 1.045)
0.209
0.003
1.003 (0.785, 1.282)
0.981
−0.179
0.836 (0.725, 0.963)
0.013
Others
−0.194
0.824 (0.719, 0.944)
0.005
0.106
1.112 (0.874, 1.414)
0.390
−0.251
0.778 (0.657, 0.923)
0.004
Mother’s occupation, ref. worker
Manager
−0.051
0.950 (0.740, 1.220)
0.688
0.219
1.244 (0.799, 1.937)
0.333
−0.268
0.765 (0.563, 1.038)
0.086
Technicist/researcher
0.153
1.165 (0.819, 1.659)
0.396
0.531
1.701 (1.024, 2.825)
0.040
−0.061
0.940 (0.570, 1.551)
0.810
Farmer
−0.141
0.868 (0.761, 0.990)
0.035
−0.035
0.965 (0.769, 1.212)
0.762
−0.155
0.856 (0.727, 1.008)
0.063
Others
−0.002
0.998 (0.881, 1.131)
0.977
−0.015
0.985 (0.774, 1.254)
0.905
−0.117
0.890 (0.767, 1.033)
0.125
Income, ref. ≤500 RMB
∼1000
−0.012
0.988 (0.775, 1.259)
0.920
−0.131
0.877 (0.383, 2.009)
0.757
0.026
1.027 (0.796, 1.325)
0.839
∼2000
−0.092
0.912 (0.727, 1.144)
0.428
−0.180
0.835 (0.396, 1.763)
0.637
0.028
1.029 (0.809, 1.308)
0.819
∼3000
−0.164
0.849 (0.679, 1.062)
0.151
−0.113
0.893 (0.433, 1.841)
0.759
0.019
1.019 (0.801, 1.296)
0.878
>3000
−0.297
0.743 (0.599, 0.921)
0.007
−0.081
0.922 (0.456, 1.866)
0.822
−0.027
0.973 (0.770, 1.231)
0.822
Living with grandparents (yes vs. no)
0.130
1.139 (0.991, 1.309)
0.066
−0.228
0.796 (0.568, 1.116)
0.186
0.120
1.127 (0.965, 1.316)
0.131
People live with child, ref. 2∼3
1
0.165
1.179 (0.931, 1.494)
0.172
0.067
1.069 (0.606, 1.886)
0.817
0.051
1.052 (0.809, 1.370)
0.704
4
0.099
1.104 (0.992, 1.229)
0.070
0.069
1.071 (0.868, 1.322)
0.522
0.035
1.035 (0.913, 1.175)
0.589
Medical insurance (yes vs. no)
−0.209
0.811 (0.707, 0.931)
0.003
0.270
1.310 (0.878, 1.954)
0.187
−0.131
0.877 (0.755, 1.019)
0.087
Gestational hypertension (yes vs. no)
0.422
1.525 (1.049, 2.218)
0.027
0.820
2.270 (1.132, 4.550)
0.021
0.232
1.261 (0.808, 1.970)
0.308
Birth weight, 100g
−0.013
0.987 (0.976, 0.998)
0.018
−0.020
0.980 (0.959, 1.002)
0.076
−0.010
0.990 (0.978, 1.003)
0.137
Breastfeeding, ref. 4∼10 months
0∼3
0.025
1.025 (0.898, 1.170)
0.714
0.223
1.250 (0.995, 1.572)
0.056
0.019
1.020 (0.863, 1.204)
0.819
>10
0.271
1.312 (1.161, 1.481)
<0.001
0.157
1.170 (0.883, 1.551)
0.275
0.175
1.191 (1.038, 1.367)
0.013
Father with obesity (yes vs. no)
0.088
1.092 (0.951, 1.254)
0.211
0.167
1.182 (0.913, 1.531)
0.205
0.072
1.075 (0.911, 1.268)
0.394
Mother with obesity (yes vs. no)
0.170
1.185 (1.007, 1.394)
0.041
0.087
1.091 (0.788, 1.510)
0.602
0.185
1.203 (0.995, 1.454)
0.057
Physical activity
Weekday, min/day
−0.055
0.947 (0.899, 0.998)
0.040
−0.027
0.973 (0.879, 1.078)
0.603
−0.037
0.964 (0.906, 1.025)
0.235
Weekend, min/day
−0.023
0.977 (0.876, 1.091)
0.682
0.081
1.084 (0.879, 1.338)
0.450
−0.001
0.999 (0.877, 1.139)
0.993
Total, min/week
−0.096
0.908 (0.819, 1.008)
0.071
−0.023
0.977 (0.796, 1.200)
0.827
−0.058
0.944 (0.835, 1.067)
0.355
Dietary intake
Pickle, g
0.002
1.002 (1.001, 1.004)
<0.001
0.001
1.001 (0.997, 1.004)
0.963
0.002
1.002 (1.000, 1.003)
0.011
aAdjusted age, gender, and height. The values in bold indicate that the variables have significant correlation with childhood hypertension only in one region, either urban or rural.


## 3.4. Effects of SES, Perinatal, Anthropometric, Biochemical, and Blood Cell Composition Measures on BP

A multivariable logistic regression model was used to analyze the relationship of SES, perinatal, anthropometric, glycolipid, and blood cell composition indexes with childhood hypertension (in Table4). In the SES model, we found that income and medical insurance had a significant impact on BP (P<0.05), explaining 0.58%, 1.13% and 0.57% of the variance of childhood hypertension in total, urban and rural areas, respectively.Table 4
Model of SES, perinatal, anthropometric, serum biochemical and blood cell composition for childhood hypertension.
Variables
Total
Urban
Rural
OR (95% CI)
P
R2
OR (95% CI)
P
R2
OR (95% CI)
P
R2
SES model
a
0.58
1.13
0.57
Education, ref. ≤9y
∼12
0.983 (0.866, 1.115)
0.787
1.039 (0.800, 1.348)
0.776
1.137 (0.981, 1.317)
0.088
∼15
0.981 (0.794, 1.212)
0.860
1.096 (0.757, 1.588)
0.627
1.087 (0.829, 1.426)
0.544
>15
1.498 (0.824, 2.724)
0.185
1.755 (0.777, 3.968)
0.176
1.466 (0.583, 3.682)
0.416
Father’s occupation, ref. worker
Manager
1.001 (0.754, 1.327)
0.999
1.574 (0.941, 2.633)
0.084
0.753 (0.533, 1.064)
0.108
Technicist/researcher
1.103 (0.922, 1.319)
0.284
1.095 (0.744, 1.611)
0.646
1.088 (0.886, 1.335)
0.420
Farmer
1.080 (0.789, 1.477)
0.631
1.996 (1.226, 3.248)
0.005
0.719 (0.451, 1.148)
0.167
Others
0.952 (0.768, 1.179)
0.650
1.268 (0.835, 1.927)
0.265
0.894 (0.692, 1.155)
0.391
Mother’s occupation, ref. worker
Manager
1.097 (0.806, 1.492)
0.557
1.062 (0.623, 1.809)
0.826
1.033 (0.704, 1.514)
0.869
Technicist/researcher
1.062 (0.893, 1.262)
0.498
1.093 (0.795, 1.502)
0.586
1.055 (0.856, 1.302)
0.614
Farmer
1.246 (0.825, 1.880)
0.296
1.147 (0.608, 2.163)
0.672
1.296 (0.748, 2.247)
0.355
Others
1.022 (0.840, 1.244)
0.825
1.110 (0.751, 1.641)
0.600
0.966 (0.765, 1.219)
0.770
Income, ref. ≤500 RMB
∼1000
1.252 (0.995, 1.575)
0.055
1.146 (0.540, 2.430)
0.723
0.993(0.774,1.274)
0.957
∼2000
1.272 (1.063, 1.523)
0.009
1.021 (0.616, 1.691)
0.936
1.046(0.856,1.278)
0.660
∼3000
1.171 (1.007, 1.362)
0.041
0.948 (0.677, 1.327)
0.755
1.036(0.868,1.235)
0.696
>3000
1.123 (0.974, 1.294)
0.111
0.991 (0.761, 1.291)
0.948
1.059 (0.891, 1.260)
0.515
Living with grandparents (yes vs. no)
1.097 (0.949, 1.267)
0.211
0.777 (0.544, 1.109)
0.165
1.119 (0.952, 1.315)
0.172
People live with child, ref. 2∼3
1
1.094 (0.855, 1.399)
0.475
1.014 (0.550, 1.868)
0.965
1.028 (0.783, 1.348)
0.845
4
1.107 (0.992, 1.235)
0.068
1.103 (0.888, 1.370)
0.377
1.054 (0.927, 1.198)
0.424
Medical insurance (yes vs. no)
1.244 (1.080, 1.433)
0.002
0.882 (0.589, 1.320)
0.541
1.147 (0.983, 1.338)
0.081
Perinatal measure model
b
0.48
0.64
0.28
Gestational hypertension (yes vs. no)
1.475 (1.008, 2.160)
0.045
2.210 (1.102, 4.432)
0.025
1.209 (0.766, 1.907)
0.414
Birth weight, ref. 3000∼3600 g
∼3000
1.142 (1.005, 1.298)
0.041
1.057 (0.825, 1.355)
0.661
1.122 (0.965, 1.304)
0.134
>3600
0.923 (0.810, 1.052)
0.231
0.796 (0.615, 1.031)
0.084
0.948 (0.813, 1.104)
0.490
Breastfeeding, ref. 4∼10 months
0∼3
1.016 (0.887, 1.164)
0.820
1.253 (0.993, 1.582)
0.057
1.017 (0.857, 1.206)
0.851
>10
1.265 (1.118, 1.433)
<0.001
1.119 (0.840, 1.489)
0.442
1.149 (0.999, 1.323)
0.052
Mother with obesity (yes vs. no)
1.140 (0.962, 1.350)
0.130
1.040 (0.742, 1.458)
0.820
1.187 (0.974, 1.447)
0.089
Anthropometric measure model
c
10.89
11.09
11.73
Weight, kg
1.040 (1.035, 1.044)
<0.001
1.046 (1.038, 1.055)
<0.001
1.038 (1.033, 1.043)
<0.001
Height, cm
0.940 (0.932, 0.948)
<0.001
0.937 (0.921, 0.952)
<0.001
0.938 (0.929, 0.947)
<0.001
Heart rate, n/min
1.089 (1.079, 1.099)
<0.001
1.087 (1.068, 1.106)
<0.001
1.096 (1.084, 1.108)
<0.001
Total PA, ref. <150 min/week
0.902 (0.810, 1.005)
0.063
1.001 (0.810, 1.236)
0.998
0.925 (0.814, 1.051)
0.230
Serum biochemical index model
d
8.86
1.19
4.09
FBG, mmol/L
1.771 (1.560, 2.012)
<0.001
1.256 (1.037, 1.521)
0.020
1.103 (0.840, 1.447)
0.480
TC, mmol/L
0.803 (0.578, 1.114)
0.189
1.123 (0.775, 1.627)
0.539
1.318(0.684,2.537)
0.409
TG, mmol/L
1.326 (1.166, 1.507)
<0.001
1.304 (1.114, 1.527)
0.001
1.504 (1.187, 1.905)
0.001
HDL-C, mmol/L
1.114 (0.725, 1.711)
0.623
0.844 (0.502, 1.419)
0.523
0.420 (0.179, 0.986)
0.046
LDL-C, mmol/L
2.262 (1.629, 3.140)
<0.001
1.124 (0.693, 1.823)
0.636
0.940 (0.507, 1.744)
0.845
Blood cell composition model
e
12.04
3.76
7.76
RBC,n × 10^12/L
3.000 (1.716, 5.243)
<0.001
1.692 (0.790, 3.622)
0.176
2.657 (1.240, 5.692)
0.012
HGB, g/L
1.012 (0.989, 1.035)
0.319
1.025 (0.958, 1.097)
0.468
1.007 (0.986, 1.029)
0.508
HCT, %
1.051 (0.964, 1.147)
0.260
1.021 (0.812, 1.283)
0.862
1.017 (0.931, 1.112)
0.702
MCV, fL
1.007 (0.980, 1.035)
0.611
0.998 (0.967, 1.030)
0.909
1.021 (0.976, 1.067)
0.370
MCHC, g/L
1.010 (0.997, 1.023)
0.117
1.003 (0.976, 1.031)
0.834
0.999 (0.978, 1.019)
0.888
PLT,n×10^9/L
1.004 (1.002, 1.005)
<0.001
1.002 (0.999, 1.004)
0.056
1.004 (1.001, 1.006)
0.004
WSCR, %
0.997 (0.971, 1.024)
0.846
1.014 (0.971, 1.059)
0.523
0.988 (0.954, 1.022)
0.476
WMCR, %
1.016 (0.951, 1.086)
0.638
1.046 (0.966, 1.133)
0.269
1.060 (0.911, 1.233)
0.450
WSCC,n×10^9/L
1.261 (0.993, 1.601)
0.057
1.153 (0.784, 1.698)
0.469
1.249 (0.917, 1.702)
0.159
WMCC,n×10^9/L
0.588 (0.259, 1.337)
0.205
0.572 (0.208, 1.570)
0.278
0.392 (0.067, 2.301)
0.300
WLCC,n×10^9/L
1.057 (0.923, 1.209)
0.424
1.173 (0.965, 1.424)
0.108
1.073 (0.874, 1.317)
0.503
RDWSD, %
1.056 (1.018, 1.096)
0.003
0.976 (0.924, 1.031)
0.388
1.015 (0.963, 1.069)
0.583
MPV, fL
1.201 (1.111, 1.298)
<0.001
1.028 (0.922, 1.147)
0.618
1.140 (0.992, 1.308)
0.064
SES, socioeconomic status; PA, physical activity; FBG, fasting blood glucose; TC, total cholesterol; TG, triglyceride; HDL-C, high-density lipoprotein cholesterol; LDL-C, low-density lipoprotein cholesterol; WBC, white blood cell; RBC, red blood cell; HGB, hemoglobin; HCT, hematocrit; MCV, mean corpuscular volume; MCHC, mean corpuscular hemoglobin concentration; PLT, platelet; WSCR, WBC small cell ratio; WMCR, WBC middle cell ratio or mixed cell percent; WSCC, WBC small cell count or lymphocyte count; WMCC, WBC middle cell count or mixed cell count; WLCC, WBC large cell count or neutrophil count; RDWSD, red blood cell distribution width; MPV, mean platelet volume.aA total of 11265, 4294, 6971 subjects being included in SES model 1 of total, urban and rural, respectively. bA total of 11171, 4328, and 6843 subjects being included in SES model 1 of total, urban, and rural areas, respectively. cA total of 11795, 4491, and 7304 subjects being included in SES model 1 of total, urban, and rural areas, respectively. dA total of 5955,4743,1212 subjects being included in SES model 1 of total, urban, and rural areas, respectively. eA total of 5662, 4521, and 1141 subjects being included in SES model 1 of total, urban, and rural areas, respectively.In the perinatal measurement model, maternal gestational hypertension was a risk factor of childhood hypertension in urban regions but not in rural regions, while >10 months of breastfeeding was a predictor of hypertension (P<0.001), especially for children in rural areas (P=0.052). The perinatal measurement model explained 0.64% and 0.28% of the variance of childhood hypertension in urban and rural areas, respectively.Weight and heart rate were risk factors of elevated BP, whereas height was a protective factor against increased BP in both regions in the anthropometric measure model. Overall, the variance of BP explained in this model was almost equal between urban and rural areas (11.09% vs 11.73%).In the serum glycolipid index model, FBG was a risk factor for BP in urban regions (P=0.02) but not in rural regions, while HDL-C was a protective factor of hypertension only in rural regions (P<0.05). In addition, TG was an important impact factor of hypertension both in urban and rural areas (all P=0.001). This model explained a total of 1.19% and 4.09% of the variance of BP in urban and rural regions, respectively.In the blood-cell composition model, RBC, PLT, RDWSD, and MPV were risk factors for high BP in total population, and RBC and PLT impact the BP level of children living in rural areas (P<0.05). The variance of BP explained in the blood-cell composition model in rural areas was higher than that in urban areas (7.76% vs 3.76%).


## 3.5. Full Model to Analyze the Risk Factors of BP

The significant variables among the SES, perinatal, anthropometric, biochemical, and blood cell composition measure models and dietary variables were included in full model 1(in Table5). Children living in rural areas have the increased odds ratio of hypertension compared with those in urban areas (2.21(1.93, 2.53), P<0.001). Pickle intake and low birth weight (<3000 g) were significantly and positively associated with hypertension only in rural regions, but maternal gestational hypertension had significant increasing effects on the BP level only in urban regions. In addition, increased BMI and heart rate were risk factors for childhood hypertension both in urban and rural regions. Full model 1 could explain 13.61%, 10.88%, and 12.23% of the variance of childhood hypertension in total, urban, and rural areas, respectively.Table 5
Full model of risk factors for childhood hypertension.
Variables
Total
Urban
Rural
OR (95% CI)
P
R2
OR (95% CI)
P
R2
OR (95% CI)
P
R2 (%)
Full model 1
a
13.61
10.88
12.23
Region (urban vs. rural)
2.208 (1.926, 2.531)
<0.001
Income, ref. ≤500 RMB
∼1000
1.052 (0.804, 1.377)
0.710
1.128 (0.424, 3.001)
0.809
1.037 (0.783, 1.373)
0.799
∼2000
1.035 (0.804, 1.332)
0.790
1.029 (0.416, 2.542)
0.951
1.040 (0.798, 1.356)
0.771
∼3000
1.004 (0.781, 1.290)
0.974
1.024 (0.422, 2.482)
0.958
1.004 (0.769, 1.310)
0.976
>3000
0.950 (0.744, 1.213)
0.682
1.016 (0.426, 2.420)
0.972
0.920 (0.709, 1.194)
0.532
Medical insurance (yes vs. no)
0.901 (0.774, 1.048)
0.177
1.173 (0.771, 1.784)
0.457
0.861 (0.730, 1.015)
0.075
Total PA, ref. <150 min/week
0.929 (0.829, 1.041)
0.202
0.927 (0.746, 1.153)
0.497
0.920 (0.805, 1.053)
0.226
Pickle, g
1.002 (1.001, 1.003)
0.025
1.001 (0.996, 1.004)
0.877
1.002 (1.001, 1.004)
0.010
Perinatal measures
Gestational hypertension (yes vs. no)
1.615 (1.083, 2.407)
0.019
2.461 (1.203, 5.036)
0.014
1.380 (0.857, 2.222)
0.186
Birth weight, ref. 3000∼3600 g
∼3000
1.152 (1.005, 1.321)
0.042
1.060 (0.814, 1.381)
0.664
1.188 (1.013, 1.394)
0.035
>3600
0.932 (0.812, 1.070)
0.315
0.839 (0.642, 1.097)
0.201
0.966 (0.821, 1.135)
0.671
Breastfeeding, ref. 4∼10 months
0∼3
1.067 (0.923, 1.232)
0.382
1.206 (0.945, 1.538)
0.133
0.991 (0.827, 1.187)
0.918
>10
1.124 (0.984, 1.284)
0.085
1.043 (0.770, 1.413)
0.786
1.134 (0.977, 1.317)
0.097
Anthropometric measures
BMI
1.184 (1.161, 1.208)
<0.001
1.151 (1.109, 1.194)
<0.001
1.199 (1.171, 1.227)
<0.001
Height, cm
0.992 (0.983, 1.002)
0.107
0.994 (0.976, 1.012)
0.514
0.992 (0.981, 1.003)
0.148
Heart rate, n/min
1.040 (1.036, 1.045)
<0.001
1.047 (1.038, 1.056)
<0.001
1.037 (1.032, 1.043)
<0.001
Full model 2
b
23.25
11.12
25.04
Region (urban vs. rural)
2.310 (1.667, 3.205)
<0.001
Income, ref. ≤500 RMB
∼1000
1.610 (0.898, 2.886)
0.328
1.021 (0.314, 3.325)
0.970
1.723 (0.873, 3.401)
0.374
∼2000
1.544 (0.896, 2.662)
0.385
1.077 (0.362, 3.209)
0.814
1.619 (0.848, 3.090)
0.537
∼3000
1.574 (0.921, 2.691)
0.261
1.105 (0.378, 3.233)
0.676
1.658(0.876,3.136)
0.423
>3000
1.373 (0.811, 2.322)
0.836
0.960(0.334,2.760)
0.625
1.492 (0.799, 2.789)
0.921
Medical insurance (yes vs. no)
0.841 (0.636, 1.112)
0.224
1.009 (0.632,1.613)
0.969
0.734 (0.509, 1.059)
0.098
Total PA, ref. <150 min/week
0.989 (0.814, 1.200)
0.908
0.907(0.703, 1.170)
0.451
1.102 (0.811, 1.498)
0.534
Pickle, g/day
1.114 (0.965, 1.287)
0.140
1.038 (0.830, 1.298)
0.742
1.177 (0.965, 1.435)
0.108
Perinatal measures
Gestational hypertension (yes vs. no)
1.939 (0.966, 3.891)
0.062
2.535 (1.117, 5.753)
0.026
1.055 (0.326, 3.421)
0.929
Birth weight, ref. 3000∼3600 g
∼3000
1.008 (0.794, 1.280)
0.759
1.057 (0.777, 1.437)
0.425
0.960 (0.657, 1.405)
0.607
>3600
0.948 (0.753, 1.195)
0.604
0.880 (0.644, 1.203)
0.303
1.106 (0.772, 1.583)
0.469
Breastfeeding, ref. 4∼10 months
0∼3
1.255 (0.993, 1.586)
0.416
1.144 (0.857, 1.526)
0.615
1.436 (0.951, 2.167)
0.425
>10
1.312 (1.035, 1.663)
0.166
1.133 (0.806, 1.595)
0.723
1.514 (1.075, 2.134)
0.156
Anthropometric measures
BMI, kg/m2
1.146 (1.112, 1.181)
<0.001
1.155 (1.102, 1.211)
<0.001
1.138 (1.092, 1.185)
<0.001
Height, cm
0.987 (0.978, 0.997)
0.009
0.975 (0.962, 0.988)
<0.001
1.006 (0.991, 1.021)
0.448
Heart rate, n/min
1.046 (1.039, 1.054)
<0.001
1.043 (1.033, 1.054)
<0.001
1.051 (1.038, 1.064)
<0.001
Biochemical index measures
FBG, mmol/L
1.080 (0.890, 1.311)
0.434
1.048 (0.820, 1.341)
0.706
1.207 (0.862, 1.689)
0.273
TC, mmol/L
0.940 (0.781, 1.132)
0.516
0.966 (0.753, 1.239)
0.785
0.859 (0.647, 1.141)
0.294
HDL-C, mmol/L
1.196 (0.783, 1.828)
0.408
1.185 (0.670, 2.096)
0.560
1.157 (0.606, 2.210)
0.658
RBC,n×10^12/L
1.946 (1.409, 2.687)
<0.001
1.742 (1.139, 2.664)
0.010
2.095 (1.239, 3.544)
0.006
HGB, g/L
1.006 (0.994, 1.018)
0.348
1.010 (0.992, 1.028)
0.274
0.999 (0.983, 1.016)
0.942
WSCR, %
1.005 (0.994, 1.017)
0.366
1.012 (0.996, 1.028)
0.128
0.996 (0.978, 1.014)
0.668
MPV, fL
1.017 (0.939, 1.101)
0.679
1.043 (0.945, 1.151)
0.408
0.954 (0.803, 1.096)
0.502
PA, physical activity; BMI, body mass index; FBG, fasting blood glucose; TC, total cholesterol; HDL-C, high-density lipoprotein cholesterol; RBC, red blood cell; HGB, hemoglobin; WSCR, white blood cell small cell ratio; MPV, mean platelet volume.aA total of 11014, 4265, and 6749 subjects being included in full model 1 of total, urban, and rural areas, respectively, and adjusted age and gender. bA total of 4265, 3257, and 1008 subjects being included in full model 2 of total, urban, and rural areas, respectively, and adjusted age and gender. The values in bold indicate that the variables have significant correlation with childhood hypertension.Glycolipid index and blood cell compositions were added in full model 2 (in Table5). In full model 2, rural residence, BMI, height, heart rate, and RBC were significant impact factors of childhood hypertension. In addition, maternal gestational hypertension was positively associated with hypertension, while height was negatively associated with BP in urban children (all P<0.05). Moreover, BMI, heart rate, and RBC were positively associated with hypertension both in urban and rural areas (all P<0.05). This model explained 23.25%, 11.12%, and 25.04% of the variance of hypertension in total, urban, and rural areas, respectively.


## 4. Discussion

The prevalence and the risk factors of childhood hypertension were assessed in this study. Our study included a variety of variables, such as perinatal, SES, lipid metabolism, and BCC, besides anthropometric variables, dietary variables, and physical activity in Southwest of China. Moreover, this study analyses the difference of the prevalence and risk factors between urban and rural areas.The prevalence of childhood hypertension was 13.75%, and the prevalence in rural areas was nearly two times higher than that in urban areas, which is consistent with the results of other studies [3, 4]. However, the urban-rural disparity of the prevalence of childhood hypertension was controversial [3, 17]. Our study found the odds ratio (OR) of hypertension was 2.31 for residence in rural areas, which was lower than the results of 4.4 from Iranian [4], whereas the differential in BP cannot be explained only by obesity, as urban children have greater BMI and waist circumference than rural children in the present study, and the urban-rural disparity existed even after adjustment of BMI and other covariates, which suggested that the urban-rural disparity was an independent risk factor of childhood hypertension. Moreover, other potential factors may provide further opportunity to illustrate this urban-rural gap. Children in rural areas have increased heart rate, glycolipid levels, and breastfeeding, and decreased SES status and physical activities compared with those in urban areas. Besides, the dietary intakes and BCC measures were different between children in urban and rural areas in the present study. The high prevalence of childhood hypertension is alarming in rural areas and requires urgent intervention to focus on the main risk factors.In this study, we found the prevalence was markedly higher in children of the age group 6∼7 compared with those in the middle age group in the present study, which was comparable with the results of another study, which found the prevalence in males of the age group 7 was higher than that in the age group 8∼10 [18], whereas most of the literature reported that the older age was a risk factor of hypertension in children [19, 20]. The results in this study may be attributed to two reasons: first, the diagnose criteria used in this study were made based on the data of 11 surveys from 2001 to 2009, and the modern lifestyle may impact the BP level, especially in the younger age group, as we found that the median BP levels of children aged 6∼7 years old in this study were significantly higher than those of the samples made the diagnose criteria (Supplementary Table 6); second, BP was measured by an OMRON arm-type electronic sphygmomanometer (HEM7051) in the present study, while mercury sphygmomanometers were used to measure BP for the reference samples, and it is more difficult to correctly hear phase 1 and phase 5 of Korotkoff sounds in younger age children than the middle age group using mercury sphygmomanometers.In this study, we also found that children with overweight/obesity have higher prevalence of hypertension, which was in consistent with other research results [21, 22]. The odds ratio of developing hypertension in overweight and obese children were 1.5 and 3.6 times more than those with a normal weight and is consistent with the results of other studies [23]. The prevalence was 37.06% in children with obesity in rural areas, which suggested that overweight/obesity is one of the strongest predictors of hypertension in childhood [9, 21, 24], and health care intervention should be conducted for children who have obesity and live in rural areas.Adverse perinatal measurements are associated with cardiovascular disease in adults, and increased BP might be one of the potential mechanisms [25, 26]. In this study, maternal gestational hypertension was a strong predictor of childhood hypertension in urban areas, which is consistent with other studies, indicating that genes and in-utero environment may account for the association. According to the literature [26–29], the present study found LBW was a major predictor of hypertension in rural children. However, some studies have reported that high birth weight is associated with an elevated risk of hypertension [30–32], while our study did not find such a correlation [33, 34]; this inconsistency may be induced by the different criteria for defining LBW and different characteristics of included samples. The correlation of breastfeeding with childhood hypertension was controversial [34–37]. The present study revealed that children with >10 months of breastfeeding had an elevated risk of hypertension, especially in rural children, whereas the literature [38, 39] reported that breastfeeding was not a predictor of blood pressure and cardiovascular disease in adults. The U-shaped relationship between duration of breastfeeding and hypertension was found [39], which agreed with our results that children with 4∼10 months of breastfeeding had the lowest prevalence of hypertension. There are 33.86% children with >10 months of breastfeeding, which may partly explain the high prevalence of hypertension in rural areas.Low SES may be a predictor of hypertension. This study confirmed this concept in rural children. In the present study, children living in rural areas have low SES, which indicated as lower education of father and income, living with grandparents, more people live together, and uncovered by medical insurance. A long-term cohort study [3]observed that children whose fathers have a low education level was a predictor of increased blood pressure in young adults, which was consistent with our results. The study from D. Rose Ewald et al. [40] found that adolescents from low-income population have significantly higher prevalence of hypertension than those from high-income population. In addition, there was higher proportion of children in rural areas live with their grandparents compared with that of children in urban areas, which is another reason to cause the urban-rural disparity of the prevalence of hypertension, which agreed with a previous study [41] from Japanese children.According to previous studies, unhealthy dietary intake [10] and micronutrient deficiency [12] are associated with childhood hypertension. Sodium intake is positively associated with increased BP and cardiovascular disease [10, 42] and is difficult to measure accurately in adulthood. Our study in children further confirmed this concept; we found that children in rural areas consumed more pickle than those living in urban and that the effect existed even after adjusting for other covariates. PA was inversely associated with the risk of hypertension [43], and Karatzi K. et al. [44] found that hypertension was positively associated with sedentary behaviors, and we found that this association existed specifically in weekday PA, suggesting that increase in the PA time for primary school children will control the blood pressure level of them.In this study, we found that the relationship between blood-cell composition and hypertension was significant. Although sufficient data are not yet available on the association between red blood cell count and hypertension, hypertensive patients have been found to have elevated RBC and high uric acid [45], and we also found that children with hypertensive had increased RBC. Hypertension may trigger the activation of platelets, for which reason most antihypertensive agents also have antiplatelet effects, but the relationship between platelet count and the degree of hypertension remains unclear [46]. Accordingly, we found that increased PLT was associated with a higher risk of hypertension in childhood in the blood-cell composition model, suggesting that hypertension may promote the presence of spontaneous platelet aggregation and consequently predict vascular occlusions even in childhood.The present study has some limitations. First, the prenatal variables were collected by a retrospective survey and the recall bias could not be eliminated. However, we checked perinatal medical records of mothers and reviewed the birth certificate of children to reduce the recall bias. Second, self-reported physical activities and dietary intake were used, which tends to be imprecise. Nevertheless, self-reported physical activities and dietary intake have been advocated in medical research. In addition, serum was drawn in only a subgroup of children. As the funding was limited, random sampling was used to control the sampling bias.In conclusion, the prevalence of hypertension was serious in children living in rural areas, especially in those with the features of overweight/obesity, living with grandparents, maternal gestational hypertension, and more pickle diet. Our study emphasizes the importance of prevention in the impact of high blood pressure at the childhood stage, and it is of great important to screen and track hypertension from childhood and adolescents, especially in rural areas. It is essential to measure the blood pressure level of children at regular time intervals in primary and middle schools, which could improve the health of children and prevent the cardiovascular disease in adults.



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*Source: 2374231-2020-05-12.xml*