# The Tissue Distribution of Four Major Coumarins after Oral Administration of Angelicae Pubescentis Radix Extract to Rats Using Ultra-High-Performance Liquid Chromatography

Authors: Yuanyuan Ge; Shujing Chen; Qian Luo; Chun-peng Wang; Jia Hao; Jun He; Xiaopeng Chen; Xuejing Yang; Jin Li; Yan-xu Chang
Journal: Evidence-Based Complementary and Alternative Medicine
 (2019)

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## Abstract

Angelicae pubescentis radix (APR) is widely applied in treating rheumatoid arthritis in China. Coumarins are the major active compounds of APR extract including columbianetin, columbianetin acetate, osthole, and columbianadin. Thein vivo behavior of the four major coumarins of APR has not been systematically reported. A feasible and reliable ultra-performance liquid chromatography (UPLC) method was established and validated for the quantification of the above four coumarins in rat various tissues (including heart, liver, spleen, lung, kidney, uterus, ovary, and muscle) after oral administration of APR extract. The separation was implemented on a Waters ACQUITY BEH C18 column (4.6 mm × 100 mm, 1.7 μm) with gradient mobile phase comprising acetonitrile-water (with 1mM formic acid) at a flow rate of 0.3 mL/min. The tissue homogenate samples were prepared by liquid-liquid extraction with ethyl acetate. The calibration curves were linear in the range of 1.6-20000 ng/mL for four coumarins with the lower limit of quantitation of 1.6 ng/mL in rat tissues. The intraday and interday precisions and recoveries were all within 80-100% with the relative standard deviations (RSDs) which were all less than 10.9%. The method was successfully applied to the tissue distribution research after oral administration of 6.0 g/kg APR extract to rat. The results revealed that the tissues distributions of four coumarins were in the liver, followed by the ovary, uterus, kidney, lung, heart, spleen, and muscle.


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## Body

## 1. Introduction

Traditional Chinese Medicine (TCM) has been crucial to prevent and treat diseases. TCM was applied to treat the illness depending on the theory of Traditional Chinese Medicine, which contains viscera-state theory [1]. According to the cure rules of chemical drugs, the tissues are the functional unit which maintains the homeostasis. In consideration of the function level, the viscera-state theory has similar effect with tissue level restoration in treating diseases. It can be seen that the tissue distribution study might act on the elucidation of healing mechanism of TCM.Angelicae pubescentis radix (APR) originates in the root ofAngelica pubescens Maxim.f. biserrata Shan et Yuan, which is primitively recorded in “Shennong’s Herbal Classic” [2]. APR possesses the efficacy of dispelling wind and removing obstruction in the meridians, activating blood and relaxation tendons, dispersing cold, and relieving pain [3], especially in treating anemofrigid-damp arthralgia. The target tissue distribution of chemical ingredients should be taken into account to find out the pharmacological mechanism of APR.Phytochemical study has demonstrated that coumarins are the main constituents in APR [4]. Previous study demonstrated that the representative coumarins of APR include the columbianetin, columbianetin acetate, osthole, and columbianadin [4, 5]. Columbianetin was found to possess the characteristic of phytoalexin [6], anti-inflammatory [7, 8], inhibitory lipid peroxidation, and platelet aggregation effects [9]. Columbianetin acetate proved to have anti-inflammatory and analgesic activities [10] and antitumor effect [11]. Osthole has the antitumor effect on ovarian cancer cells [12], estrogen-like activity in MCF-7 cells, and stimulatory effect on ALP activity of Saos-2 osteoblast cells [13], is a dual agonist of peroxisome proliferator-activated receptor (PPAR) α
/
γ, and decreases the hepatic lipid accumulation [14]. Columbianadin was certified to show the inhibitory macrophage activation, stabilize the detachment of alveolar macrophages [15], and induce apoptosis and necroptosis in HCT116 colon cancer cells [16]. Therefore, the four representative active coumarins in APR play essential roles in disease treatment. The research ofin vivo behavior of these coumarins is crucial to the understanding of the pharmacological activity of APR extract.Although the pharmacokinetics or tissue distributions of osthole, columbianadin, and columbianetin in other plant extracts have been studied in previous literature [17–20], the tissue distribution of holistic representative coumarins in APR has not been reported. In order to explore the relation of viscera-state theory and tissue contribution of APR for further illuminating the mechanism, a sensitive, precise, and reliable UPLC method was established to test simultaneously the concentration of columbianetin, columbianetin acetate, osthole, and columbianadin in different tissues after oral APR extract to rat tissues.


## 2. Experimental

### 2.1. Chemicals and Reagents

Columbianetin, columbianetin acetate, osthole, and columbianadin were obtained from National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). Acetonitrile (HPLC grade) was purchased from Dikma Technologies Inc. (Dikma, USA). Methanol (HPLC grade) and ethyl acetate (analytical grade) were purchased from Tianjin Concord Science Co. Ltd. (Tianjin, China). Formic acid was purchased from Merck (Darmstadt, Germany). Deionized water was purified with a Milli-Q Academic ultra-pure water system (Millipore, Milford, MA, USA).


### 2.2. Instrument and Analytical Conditions

The liquid chromatography of Waters UPLC (Waters, America) system was used with the photodiode array detection (PDA). Chromatographic separation was performed on an ACQUITY BEH C18 column (4.6 mm × 100 mm, 1.7μm) with a security VanGuard C18 column (2.1 mm × 12.5 mm, 1.7 μm). The column oven temperature was maintained at 25°C. The mobile phase was composed of acetonitrile (A) and aqueous formic acid (1mM) (B) using gradient elution as follows: 40% A at 0-5 min, 40-70% A at 5-10 min, 70-75% A at 10-11 min, 75-90% A at 11-19 min, 90% A at 19-22 min, and 90-40% A at 22-29 min. The flow rate was 0.3 mL/min and the injection volume was 5 μL. The wavelength was set at 320 nm for the four coumarins.


### 2.3. Preparation of APR Extract

For the preparation of the extract, 2 kg APR was cut into pieces and soaked with 75% ethanol (16L) for 2 h. The filtrate was concentrated by using a rotary evaporator under reduced pressure. The dry APR extract (100 mg) was accurately weighed in triplicate. The sample was dissolved with 5 mL of 70% methanol by using an ultrasonic bath for 30min and then cooled at room temperature. The supernatant of 2 mg/mL APR extract was injected into the UPLC-PDA system. The chromatography conditions of UPLC-PDA for separation and determination of four coumarins in APR extract were slightly modified according to previous studies [21]. The mobile phase consisted of acetonitrile (A) and 0.1% formic acid/water (B) set as follows: 0–2 min, 5–8% A; 2–5 min, 8–10% A; 5–8 min, 10–14% A; 8–9 min, 14–16% A; 9–12 min, 16–16% A; 12–13 min, 16–18% A; 13–14 min, 18–20% A; 14–15 min, 20–21% A; 15–18 min, 21–23% A; 18–21 min, 23–30% A; 21–23 min, 30–50% A; 23–28 min, 50–51% A; 28–29 min, 51–95% A; and 29-30 min, 95-5% A. The injection volume was 2 μL. The fingerprint of APR extract was shown in Figure 1. The standard concentrations ranges were 0.4–50 μg/mL for columbianetin, 1.6–200 μg/mL for columbianetin acetate, 4.0–500 μg/mL for osthole, and 1.2–150 μg/mL for columbianadin, respectively. The contents of columbianetin, columbianetin acetate, osthole, and columbianadin were 0.4842 mg/g, 3.559 mg/g, 14.63 mg/g, and 1.059 mg/g in APR extract, respectively. The osthole was the most abundant coumarin in APR extract.Figure 1
The UHPLC-PDA chromatograms with APR extract sample (a) and standard solution of four compounds (b). 1: columbianetin, 2: columbianetin acetate, 3: osthole, 4: columbianadin.
(a)
(b)


### 2.4. Preparation of Stock Solutions and Quality Control Samples

Four stock solutions of columbianetin, columbianetin acetate, osthole, and columbianadin (1 mg/mL) were prepared in methanol and stored in 4°C, individually. The chemical structures of columbianetin, columbianetin acetate, osthole, and columbianadin are shown in Figure2. The stock solutions of above compounds were stepwise diluted with methanol, and then the diluents were added to blank tissues to yield final concentrations of 1.6, 8, 40, 200, 500, 2500, 10000, and 20000 ng/mL. Quality control (QC) samples were prepared with blank tissues at the concentrations of 160, 500, and 2000 ng/mL for osthole and columbianadin; 80, 400, and 2000 ng/mL for columbianetin acetate; 80, 500, and 2000 ng/mL for columbianetin, respectively.Figure 2
Chemical structures of columbianetin, columbianetin acetate, osthole, and columbianadin.


### 2.5. Preparation of Tissue Samples

The tissue samples were homogenized with 2-fold volume of 0.9% normal saline. The tissue homogenate was processed by liquid-liquid extraction. An aliquot of 200μL tissue homogenate was put into 1.5 mL eppendorf tube. The 1000 μL of ethyl acetate was added to extract the four analytes. The tube was vortex-mixed for 1 min and centrifuged at 14000 rpm for 10 min. The supernatant was transferred into another tube and evaporated to dryness under nitrogen at 40°C. The dried residue was reconstituted in 80 μL of methanol. After swirling for 1 min, oscillating ultrasonically for 3 min, and centrifuging at 14000 rpm for 10 min, 5 μL aliquot of the supernatant was injected into UPLC for analysis.


### 2.6. Method Validation

According to the USFDA bioanalytical method validation guidelines [22], the method was validated in terms of specificity, sensitivity, linearity, accuracy, precision, and recovery. The specificity was assessed using blank tissue samples, blank tissue spiked with standard analytes, and real tissue samples after oral administration of APR extract. Lower limit of quantitation (LLOQ) means the concentration of minimum signal to noise ratio was at least more than five for biosamples. Calibration curve samples of four coumarins were handled with blank rat tissue homogenate at the concentrations of 1.6, 8, 40, 200, 500, 2500, 10000, and 20000 ng/mL. The stand curve was gained by plotting the peak area (y) ratio of nominal concentration (x) with least-squares linear regression using 1/x2 as weighing factor. The precision and accuracy were calculated by determining QC samples of low, middle, and high levels. The precision was expressed by the relative standard deviations (RSDs), and the accuracy was represented by the calculated concentration ratio of known concentration. The extraction recovery was described by comparing peak areas of analytes from extracted samples with the postextracted spiked samples. The stability of processed samples was estimated by analyzing the target compounds which were kept in an autosampler for 24 h.


### 2.7. Application to Tissue Distribution Study

Female Sprague-Dawley rats (250~280g) were fed under standard housing conditions and were acclimated at breeding room for at least one week beforehand. The rats were fasted for 12h before the experiment. All rats experiments were realized in strict accordance with the Guidelines for the Care and Use of Laboratory Animals and were approved by the Animal Ethics Committee of Tianjin University of Traditional Chinese Medicine (Tianjin, China). The 18 rats were divided randomly into three groups (n=6 per group). The rats were sacrificed by cervical dislocation at 4, 6, and 8 h after oral administration of 6 g/kg APR extract, respectively. The heart, liver, spleen, lung, kidney, uterus, ovary, and muscle were rapidly excised, rinsed, wiped, and weighed. All tissues were stored at -80°C until analysis.


## 2.1. Chemicals and Reagents

Columbianetin, columbianetin acetate, osthole, and columbianadin were obtained from National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). Acetonitrile (HPLC grade) was purchased from Dikma Technologies Inc. (Dikma, USA). Methanol (HPLC grade) and ethyl acetate (analytical grade) were purchased from Tianjin Concord Science Co. Ltd. (Tianjin, China). Formic acid was purchased from Merck (Darmstadt, Germany). Deionized water was purified with a Milli-Q Academic ultra-pure water system (Millipore, Milford, MA, USA).


## 2.2. Instrument and Analytical Conditions

The liquid chromatography of Waters UPLC (Waters, America) system was used with the photodiode array detection (PDA). Chromatographic separation was performed on an ACQUITY BEH C18 column (4.6 mm × 100 mm, 1.7μm) with a security VanGuard C18 column (2.1 mm × 12.5 mm, 1.7 μm). The column oven temperature was maintained at 25°C. The mobile phase was composed of acetonitrile (A) and aqueous formic acid (1mM) (B) using gradient elution as follows: 40% A at 0-5 min, 40-70% A at 5-10 min, 70-75% A at 10-11 min, 75-90% A at 11-19 min, 90% A at 19-22 min, and 90-40% A at 22-29 min. The flow rate was 0.3 mL/min and the injection volume was 5 μL. The wavelength was set at 320 nm for the four coumarins.


## 2.3. Preparation of APR Extract

For the preparation of the extract, 2 kg APR was cut into pieces and soaked with 75% ethanol (16L) for 2 h. The filtrate was concentrated by using a rotary evaporator under reduced pressure. The dry APR extract (100 mg) was accurately weighed in triplicate. The sample was dissolved with 5 mL of 70% methanol by using an ultrasonic bath for 30min and then cooled at room temperature. The supernatant of 2 mg/mL APR extract was injected into the UPLC-PDA system. The chromatography conditions of UPLC-PDA for separation and determination of four coumarins in APR extract were slightly modified according to previous studies [21]. The mobile phase consisted of acetonitrile (A) and 0.1% formic acid/water (B) set as follows: 0–2 min, 5–8% A; 2–5 min, 8–10% A; 5–8 min, 10–14% A; 8–9 min, 14–16% A; 9–12 min, 16–16% A; 12–13 min, 16–18% A; 13–14 min, 18–20% A; 14–15 min, 20–21% A; 15–18 min, 21–23% A; 18–21 min, 23–30% A; 21–23 min, 30–50% A; 23–28 min, 50–51% A; 28–29 min, 51–95% A; and 29-30 min, 95-5% A. The injection volume was 2 μL. The fingerprint of APR extract was shown in Figure 1. The standard concentrations ranges were 0.4–50 μg/mL for columbianetin, 1.6–200 μg/mL for columbianetin acetate, 4.0–500 μg/mL for osthole, and 1.2–150 μg/mL for columbianadin, respectively. The contents of columbianetin, columbianetin acetate, osthole, and columbianadin were 0.4842 mg/g, 3.559 mg/g, 14.63 mg/g, and 1.059 mg/g in APR extract, respectively. The osthole was the most abundant coumarin in APR extract.Figure 1
The UHPLC-PDA chromatograms with APR extract sample (a) and standard solution of four compounds (b). 1: columbianetin, 2: columbianetin acetate, 3: osthole, 4: columbianadin.
(a)
(b)


## 2.4. Preparation of Stock Solutions and Quality Control Samples

Four stock solutions of columbianetin, columbianetin acetate, osthole, and columbianadin (1 mg/mL) were prepared in methanol and stored in 4°C, individually. The chemical structures of columbianetin, columbianetin acetate, osthole, and columbianadin are shown in Figure2. The stock solutions of above compounds were stepwise diluted with methanol, and then the diluents were added to blank tissues to yield final concentrations of 1.6, 8, 40, 200, 500, 2500, 10000, and 20000 ng/mL. Quality control (QC) samples were prepared with blank tissues at the concentrations of 160, 500, and 2000 ng/mL for osthole and columbianadin; 80, 400, and 2000 ng/mL for columbianetin acetate; 80, 500, and 2000 ng/mL for columbianetin, respectively.Figure 2
Chemical structures of columbianetin, columbianetin acetate, osthole, and columbianadin.


## 2.5. Preparation of Tissue Samples

The tissue samples were homogenized with 2-fold volume of 0.9% normal saline. The tissue homogenate was processed by liquid-liquid extraction. An aliquot of 200μL tissue homogenate was put into 1.5 mL eppendorf tube. The 1000 μL of ethyl acetate was added to extract the four analytes. The tube was vortex-mixed for 1 min and centrifuged at 14000 rpm for 10 min. The supernatant was transferred into another tube and evaporated to dryness under nitrogen at 40°C. The dried residue was reconstituted in 80 μL of methanol. After swirling for 1 min, oscillating ultrasonically for 3 min, and centrifuging at 14000 rpm for 10 min, 5 μL aliquot of the supernatant was injected into UPLC for analysis.


## 2.6. Method Validation

According to the USFDA bioanalytical method validation guidelines [22], the method was validated in terms of specificity, sensitivity, linearity, accuracy, precision, and recovery. The specificity was assessed using blank tissue samples, blank tissue spiked with standard analytes, and real tissue samples after oral administration of APR extract. Lower limit of quantitation (LLOQ) means the concentration of minimum signal to noise ratio was at least more than five for biosamples. Calibration curve samples of four coumarins were handled with blank rat tissue homogenate at the concentrations of 1.6, 8, 40, 200, 500, 2500, 10000, and 20000 ng/mL. The stand curve was gained by plotting the peak area (y) ratio of nominal concentration (x) with least-squares linear regression using 1/x2 as weighing factor. The precision and accuracy were calculated by determining QC samples of low, middle, and high levels. The precision was expressed by the relative standard deviations (RSDs), and the accuracy was represented by the calculated concentration ratio of known concentration. The extraction recovery was described by comparing peak areas of analytes from extracted samples with the postextracted spiked samples. The stability of processed samples was estimated by analyzing the target compounds which were kept in an autosampler for 24 h.


## 2.7. Application to Tissue Distribution Study

Female Sprague-Dawley rats (250~280g) were fed under standard housing conditions and were acclimated at breeding room for at least one week beforehand. The rats were fasted for 12h before the experiment. All rats experiments were realized in strict accordance with the Guidelines for the Care and Use of Laboratory Animals and were approved by the Animal Ethics Committee of Tianjin University of Traditional Chinese Medicine (Tianjin, China). The 18 rats were divided randomly into three groups (n=6 per group). The rats were sacrificed by cervical dislocation at 4, 6, and 8 h after oral administration of 6 g/kg APR extract, respectively. The heart, liver, spleen, lung, kidney, uterus, ovary, and muscle were rapidly excised, rinsed, wiped, and weighed. All tissues were stored at -80°C until analysis.


## 3. Results

### 3.1. Method Validation

#### 3.1.1. Specificity and Selectivity

The representative chromatograms consist of blank tissues homogenate, blank biosample with standard substances, and tissues samples after oral administration of APR extract as shown in Figure3. The results revealed that the retention time of columbianetin, columbianetin acetate, osthole, and columbianadin was 10.9, 19.0, 21.3, and 22.4 min, respectively. As shown in the chromatograms, there are no obvious endogenous substances and metabolite to disturb the quantification of the four coumarins.Figure 3
Representative tissue chromatogram of (a) blank heart tissue, (b) blank heart tissue spiked with the standard compounds at LLOQ, and (c) real samples after administration of APR extract. 1: columbianetin, 2: columbianetin acetate, 3: osthole, 4: columbianadin.
(a)
(b)
(c)


#### 3.1.2. Linearity and LLOQ

The calibration curves were over the range of 1.6-20000 ng/mL, and the correlation coefficients (r) were more than 0.99. These indicated that calibration curves exhibited good linearity to quantify the four coumarins. The lower limit of quantitation (LLOQ) of four analytes was set at 1.6 ng/mL with the S/N value approximating 5.


#### 3.1.3. Precision and Accuracy

The results of the precision and accuracy of the four analytes are summarized in Table1. The intraday precisions in eight tissues ranged from 0.35% to 5.77%, and corresponding accuracies ranged from 89.2% to 97.3%. The interday precisions ranged from 0.85% to 8.71%, and corresponding accuracies ranged from 90.4% to 98.3%. The data revealed that the method was precise and accurate for quantitation of four coumarins in tissue samples.Table 1
The precision and stabilities of columbianetin acetate, columbianetin, osthole, and columbianadin.
Concentration(ng/mL)
Intra-day precision
Inter-day precision
Autosampler for 24 hours stability
Accuracy(%)
RSD(%)
Accuracy(%)
RSD(%)
Accuracy(%)
RSD(%)
Columbianetin
80
95.9
5.20
94.2
8.71
99.8
7.83
500
93.9
0.35
96.2
2.11
97.9
2.89
2000
93.8
0.74
97.3
1.91
96.0
9.48
Columbianetin acetate
80
93.6
3.05
93.5
7.32
97.4
2.55
400
92.6
3.04
95.6
0.85
96.7
7.63
2000
91.8
5.71
90.4
1.89
85.4
3.14
Osthole
160
97.3
5.29
98.3
4.32
99.2
5.28
500
89.2
0.91
91.6
1.67
97.1
12.8
2000
91.9
2.68
93.6
2.64
88.4
5.39
Columbianadin
160
92.6
5.77
93.8
6.54
97.1
5.73
500
93.4
0.51
95.8
2.32
99.1
3.30
2000
93.7
0.74
96.9
1.47
95.6
8.74


#### 3.1.4. Recovery

The results of recovery are listed in Table2. The extraction recoveries of analytes in eight tissues were within the range of 80.2%-99.9%, and corresponding RSDs are less than 15%. The data indicated that the interference could be neglected in rat tissue sample.Table 2
The recoveries of columbianetin, columbianetin acetate, osthole, and columbianadin.
Tissues
columbianetin
columbianetin acetate
Low
Middle
High
Low
Middle
High
Recovery
RSD
Recovery
RSD
Recovery
RSD
Recovery
RSD
Recovery
RSD
Recovery
RSD
(%)
(%)
(%)
(%)
(%)
(%)
(%)
(%)
(%)
(%)
(%)
(%)
Heart
81.8
3.96
94.6
1.72
93.8
0.69
92.8
3.80
82.8
4.74
83.8
2.55
Liver
87.8
2.67
88.7
0.72
94.2
1.57
85.7
7.03
82.1
10.5
87.1
2.97
Spleen
89.5
4.09
99.1
1.55
97.4
1.93
80.8
9.63
80.7
3.92
93.2
1.19
Lung
97.7
2.58
99.4
8.71
97.8
1.46
84.3
10.3
85.8
9.46
86.1
5.15
Kidney
97.4
4.64
95.8
5.80
95.5
0.61
84.6
4.71
81.1
4.75
91.7
3.02
Uterus
93.0
8.54
95.7
8.83
97.3
3.65
97.6
1.02
86.2
4.13
88.4
7.43
Ovary
99.3
5.82
92.6
3.28
97.9
7.40
83.1
10.4
85.7
5.75
89.5
5.21
Muscle
92.1
6.42
93.9
0.35
93.8
0.74
91.3
6.04
98.3
3.22
90.1
5.71
Tissues
osthole
columbianadin
Low
Middle
High
Low
Middle
High
Recovery
RSD
Recovery
RSD
Recovery
RSD
Recovery
RSD
Recovery
RSD
Recovery
RSD
(%)
(%)
(%)
(%)
(%)
(%)
(%)
(%)
(%)
(%)
(%)
(%)
Heart
91.2
7.70
98.6
2.75
94.3
3.55
82.1
1.83
80.2
4.73
88.4
1.13
Liver
93.1
4.42
94.6
5.87
93.1
5.93
81.3
4.84
81.9
1.92
84.3
5.68
Spleen
95.1
6.40
95.7
7.61
90.9
3.33
81.0
3.11
83.1
6.19
91.9
2.00
Lung
94.3
9.37
99.8
1.38
92.3
5.74
96.5
7.32
89.0
1.32
90.0
3.05
Kidney
94.9
10.88
97.1
4.08
95.5
7.42
92.2
1.73
95.3
1.26
91.4
0.81
Uterus
88.2
10.51
96.5
2.33
99.9
5.11
94.3
9.57
99.2
2.72
96.5
7.75
Ovary
93.8
3.76
94.7
6.11
93.9
5.63
96.8
3.41
98.4
4.09
98.4
7.47
Muscle
97.3
5.29
89.2
0.90
91.9
2.68
92.6
5.77
98.2
4.76
97.6
3.20


#### 3.1.5. Stability

The stability of processed samples which were kept in an autosampler for 24 h is shown in Table1. The accuracy ranged from 88.4% to 99.8%, and corresponding RSD ranged from 2.55% to 12.8%. The data were well within the acceptable limit. The results demonstrated that the four compounds were stable under the detected conditions.


### 3.2. Tissue Distribution Studies

The tissue distributions of the four coumarins were investigated at 4, 6, and 8 h after given orally 6.0 g/kg APR extract to female rats. The examined tissues included the heart, liver, spleen, lung, kidney, uterus, ovary, and muscle. The concentrations of four coumarins in tissues are presented in Table3. The mean concentration of four analytes at different tissues and points-in-time are shown in Figure 4. The highest and lowest concentrations of four coumarins were observed at 6 h and at 8 h in different tissues from 4 h to 8 h, respectively. The phenomenon indicated that the distribution tendency of four coumarins rose first then fell in most tissues. The reason that the highest concentration of four coumarins was detected in the liver at 6 h after oral administration APR extract was related to the liver in which the drugs metabolism mainly takes place. The concentration of columbianetin was higher than those of other constituents in muscle, which implied that columbianetin might be the main activity constituent in APR to cure arthrophlogosis. The relative higher distribution in kidney suggested that it might be the primary excretion tissue of the columbianetin. On the whole, the concentration of columbianetin was higher than other compounds, and the concentration order of four coumarins was columbianetin>osthole>columbianetin acetate > columbianadin in eight tissues, successively. The concentration of columbianetin acetate and osthole in uterus and ovary was higher than those in other tissues, which probably implied that both components exert estrogen-like effect in uterus and ovary. This result deeply proved the effect of estrogen-like activity of osthole in MCF-7 cells.Table 3
The concentrations of columbianetin, columbianetin acetate, osthole, and columbianadin in tissues (ng/mL)(n=6).
Tissues
columbianetin
columbianetin acetate
osthole
columbianadin
4h
6h
8h
4h
6h
8h
4h
6h
8h
4h
6h
8h
Heart
735.0±316.5
814.6±192.3
86.9±80.2
77.4±41.2
175.0±50.0
45.3±40.7
183.5±56.0
227.4±39.3
135.5±28.6
0.00±0.00
54.3±36.8
0.0±0.0
Liver
1467.0±913.4
2032±514
325.4±262.5
183.3±22.2
1677.0±42.4
94.0±86.4
669.3±286.5
1352±529.0
257.8±24.3
273.2±185.4
908.4±738.6
46.8±51.3
Spleen
533.1±266.9
529.1±243.6
171.0±9.9
47.4±30.6
180.9±0.0
42.8±53.4
191.2±48.5
206.6±67.6
153.5±42.6
0.00±0.00
0.00±0.00
12.2±24.4
Lung
653.0±306.1
647.4±227.5
95.3±72.0
76.6±46.9
94.5±70.9
16.9±17.7
275.0±101.6
188.3±49.0
125.8±24.7
0.00±0.00
0.00±0.00
0.0±0.0
Kidney
810.3±460.5
933.7±295.8
222.1±41.5
104.7±45.7
180.9±0.0
50.5±40.9
221.2±86.1
264.1±19.6
162.5±38.2
136.3±60.9
0.00±0.00
142.2±47.4
Uterus
565.1±129.8
582.6±130.5
155.1±34.8
140.5±49.2
428.3±47.0
77.7±76.9
261.0±57.0
382.6±83.5
167.0±42.3
133.7±44.6
0.00±0.00
0.00±0.00
Ovary
614.6±341.2
604.9±132.2
91.4±16.3
204.8±42.6
292.9±145.0
98.6±38.8
358.5±78.3
403.5±97.6
224.0±20.2
118.7±68.5
126.0±9.7
0.00±0.00
Muscle
712.2±176.4
663.3±239.3
117.4 ±92.8
55.0±10.7
79.7±49.4
32.9±31.7
154.7±9.7
118.1±82.9
126.4±7.0
0.00±0.00
0.0±0.0
0.00±0.00Figure 4
The tendency graph of columbianetin, columbianetin acetate, osthole, and columbianadin in eight tissues at 4, 6, and 8 h after oral administration of APR extract (n=6).


## 3.1. Method Validation

### 3.1.1. Specificity and Selectivity

The representative chromatograms consist of blank tissues homogenate, blank biosample with standard substances, and tissues samples after oral administration of APR extract as shown in Figure3. The results revealed that the retention time of columbianetin, columbianetin acetate, osthole, and columbianadin was 10.9, 19.0, 21.3, and 22.4 min, respectively. As shown in the chromatograms, there are no obvious endogenous substances and metabolite to disturb the quantification of the four coumarins.Figure 3
Representative tissue chromatogram of (a) blank heart tissue, (b) blank heart tissue spiked with the standard compounds at LLOQ, and (c) real samples after administration of APR extract. 1: columbianetin, 2: columbianetin acetate, 3: osthole, 4: columbianadin.
(a)
(b)
(c)


### 3.1.2. Linearity and LLOQ

The calibration curves were over the range of 1.6-20000 ng/mL, and the correlation coefficients (r) were more than 0.99. These indicated that calibration curves exhibited good linearity to quantify the four coumarins. The lower limit of quantitation (LLOQ) of four analytes was set at 1.6 ng/mL with the S/N value approximating 5.


### 3.1.3. Precision and Accuracy

The results of the precision and accuracy of the four analytes are summarized in Table1. The intraday precisions in eight tissues ranged from 0.35% to 5.77%, and corresponding accuracies ranged from 89.2% to 97.3%. The interday precisions ranged from 0.85% to 8.71%, and corresponding accuracies ranged from 90.4% to 98.3%. The data revealed that the method was precise and accurate for quantitation of four coumarins in tissue samples.Table 1
The precision and stabilities of columbianetin acetate, columbianetin, osthole, and columbianadin.
Concentration(ng/mL)
Intra-day precision
Inter-day precision
Autosampler for 24 hours stability
Accuracy(%)
RSD(%)
Accuracy(%)
RSD(%)
Accuracy(%)
RSD(%)
Columbianetin
80
95.9
5.20
94.2
8.71
99.8
7.83
500
93.9
0.35
96.2
2.11
97.9
2.89
2000
93.8
0.74
97.3
1.91
96.0
9.48
Columbianetin acetate
80
93.6
3.05
93.5
7.32
97.4
2.55
400
92.6
3.04
95.6
0.85
96.7
7.63
2000
91.8
5.71
90.4
1.89
85.4
3.14
Osthole
160
97.3
5.29
98.3
4.32
99.2
5.28
500
89.2
0.91
91.6
1.67
97.1
12.8
2000
91.9
2.68
93.6
2.64
88.4
5.39
Columbianadin
160
92.6
5.77
93.8
6.54
97.1
5.73
500
93.4
0.51
95.8
2.32
99.1
3.30
2000
93.7
0.74
96.9
1.47
95.6
8.74


### 3.1.4. Recovery

The results of recovery are listed in Table2. The extraction recoveries of analytes in eight tissues were within the range of 80.2%-99.9%, and corresponding RSDs are less than 15%. The data indicated that the interference could be neglected in rat tissue sample.Table 2
The recoveries of columbianetin, columbianetin acetate, osthole, and columbianadin.
Tissues
columbianetin
columbianetin acetate
Low
Middle
High
Low
Middle
High
Recovery
RSD
Recovery
RSD
Recovery
RSD
Recovery
RSD
Recovery
RSD
Recovery
RSD
(%)
(%)
(%)
(%)
(%)
(%)
(%)
(%)
(%)
(%)
(%)
(%)
Heart
81.8
3.96
94.6
1.72
93.8
0.69
92.8
3.80
82.8
4.74
83.8
2.55
Liver
87.8
2.67
88.7
0.72
94.2
1.57
85.7
7.03
82.1
10.5
87.1
2.97
Spleen
89.5
4.09
99.1
1.55
97.4
1.93
80.8
9.63
80.7
3.92
93.2
1.19
Lung
97.7
2.58
99.4
8.71
97.8
1.46
84.3
10.3
85.8
9.46
86.1
5.15
Kidney
97.4
4.64
95.8
5.80
95.5
0.61
84.6
4.71
81.1
4.75
91.7
3.02
Uterus
93.0
8.54
95.7
8.83
97.3
3.65
97.6
1.02
86.2
4.13
88.4
7.43
Ovary
99.3
5.82
92.6
3.28
97.9
7.40
83.1
10.4
85.7
5.75
89.5
5.21
Muscle
92.1
6.42
93.9
0.35
93.8
0.74
91.3
6.04
98.3
3.22
90.1
5.71
Tissues
osthole
columbianadin
Low
Middle
High
Low
Middle
High
Recovery
RSD
Recovery
RSD
Recovery
RSD
Recovery
RSD
Recovery
RSD
Recovery
RSD
(%)
(%)
(%)
(%)
(%)
(%)
(%)
(%)
(%)
(%)
(%)
(%)
Heart
91.2
7.70
98.6
2.75
94.3
3.55
82.1
1.83
80.2
4.73
88.4
1.13
Liver
93.1
4.42
94.6
5.87
93.1
5.93
81.3
4.84
81.9
1.92
84.3
5.68
Spleen
95.1
6.40
95.7
7.61
90.9
3.33
81.0
3.11
83.1
6.19
91.9
2.00
Lung
94.3
9.37
99.8
1.38
92.3
5.74
96.5
7.32
89.0
1.32
90.0
3.05
Kidney
94.9
10.88
97.1
4.08
95.5
7.42
92.2
1.73
95.3
1.26
91.4
0.81
Uterus
88.2
10.51
96.5
2.33
99.9
5.11
94.3
9.57
99.2
2.72
96.5
7.75
Ovary
93.8
3.76
94.7
6.11
93.9
5.63
96.8
3.41
98.4
4.09
98.4
7.47
Muscle
97.3
5.29
89.2
0.90
91.9
2.68
92.6
5.77
98.2
4.76
97.6
3.20


### 3.1.5. Stability

The stability of processed samples which were kept in an autosampler for 24 h is shown in Table1. The accuracy ranged from 88.4% to 99.8%, and corresponding RSD ranged from 2.55% to 12.8%. The data were well within the acceptable limit. The results demonstrated that the four compounds were stable under the detected conditions.


## 3.1.1. Specificity and Selectivity

The representative chromatograms consist of blank tissues homogenate, blank biosample with standard substances, and tissues samples after oral administration of APR extract as shown in Figure3. The results revealed that the retention time of columbianetin, columbianetin acetate, osthole, and columbianadin was 10.9, 19.0, 21.3, and 22.4 min, respectively. As shown in the chromatograms, there are no obvious endogenous substances and metabolite to disturb the quantification of the four coumarins.Figure 3
Representative tissue chromatogram of (a) blank heart tissue, (b) blank heart tissue spiked with the standard compounds at LLOQ, and (c) real samples after administration of APR extract. 1: columbianetin, 2: columbianetin acetate, 3: osthole, 4: columbianadin.
(a)
(b)
(c)


## 3.1.2. Linearity and LLOQ

The calibration curves were over the range of 1.6-20000 ng/mL, and the correlation coefficients (r) were more than 0.99. These indicated that calibration curves exhibited good linearity to quantify the four coumarins. The lower limit of quantitation (LLOQ) of four analytes was set at 1.6 ng/mL with the S/N value approximating 5.


## 3.1.3. Precision and Accuracy

The results of the precision and accuracy of the four analytes are summarized in Table1. The intraday precisions in eight tissues ranged from 0.35% to 5.77%, and corresponding accuracies ranged from 89.2% to 97.3%. The interday precisions ranged from 0.85% to 8.71%, and corresponding accuracies ranged from 90.4% to 98.3%. The data revealed that the method was precise and accurate for quantitation of four coumarins in tissue samples.Table 1
The precision and stabilities of columbianetin acetate, columbianetin, osthole, and columbianadin.
Concentration(ng/mL)
Intra-day precision
Inter-day precision
Autosampler for 24 hours stability
Accuracy(%)
RSD(%)
Accuracy(%)
RSD(%)
Accuracy(%)
RSD(%)
Columbianetin
80
95.9
5.20
94.2
8.71
99.8
7.83
500
93.9
0.35
96.2
2.11
97.9
2.89
2000
93.8
0.74
97.3
1.91
96.0
9.48
Columbianetin acetate
80
93.6
3.05
93.5
7.32
97.4
2.55
400
92.6
3.04
95.6
0.85
96.7
7.63
2000
91.8
5.71
90.4
1.89
85.4
3.14
Osthole
160
97.3
5.29
98.3
4.32
99.2
5.28
500
89.2
0.91
91.6
1.67
97.1
12.8
2000
91.9
2.68
93.6
2.64
88.4
5.39
Columbianadin
160
92.6
5.77
93.8
6.54
97.1
5.73
500
93.4
0.51
95.8
2.32
99.1
3.30
2000
93.7
0.74
96.9
1.47
95.6
8.74


## 3.1.4. Recovery

The results of recovery are listed in Table2. The extraction recoveries of analytes in eight tissues were within the range of 80.2%-99.9%, and corresponding RSDs are less than 15%. The data indicated that the interference could be neglected in rat tissue sample.Table 2
The recoveries of columbianetin, columbianetin acetate, osthole, and columbianadin.
Tissues
columbianetin
columbianetin acetate
Low
Middle
High
Low
Middle
High
Recovery
RSD
Recovery
RSD
Recovery
RSD
Recovery
RSD
Recovery
RSD
Recovery
RSD
(%)
(%)
(%)
(%)
(%)
(%)
(%)
(%)
(%)
(%)
(%)
(%)
Heart
81.8
3.96
94.6
1.72
93.8
0.69
92.8
3.80
82.8
4.74
83.8
2.55
Liver
87.8
2.67
88.7
0.72
94.2
1.57
85.7
7.03
82.1
10.5
87.1
2.97
Spleen
89.5
4.09
99.1
1.55
97.4
1.93
80.8
9.63
80.7
3.92
93.2
1.19
Lung
97.7
2.58
99.4
8.71
97.8
1.46
84.3
10.3
85.8
9.46
86.1
5.15
Kidney
97.4
4.64
95.8
5.80
95.5
0.61
84.6
4.71
81.1
4.75
91.7
3.02
Uterus
93.0
8.54
95.7
8.83
97.3
3.65
97.6
1.02
86.2
4.13
88.4
7.43
Ovary
99.3
5.82
92.6
3.28
97.9
7.40
83.1
10.4
85.7
5.75
89.5
5.21
Muscle
92.1
6.42
93.9
0.35
93.8
0.74
91.3
6.04
98.3
3.22
90.1
5.71
Tissues
osthole
columbianadin
Low
Middle
High
Low
Middle
High
Recovery
RSD
Recovery
RSD
Recovery
RSD
Recovery
RSD
Recovery
RSD
Recovery
RSD
(%)
(%)
(%)
(%)
(%)
(%)
(%)
(%)
(%)
(%)
(%)
(%)
Heart
91.2
7.70
98.6
2.75
94.3
3.55
82.1
1.83
80.2
4.73
88.4
1.13
Liver
93.1
4.42
94.6
5.87
93.1
5.93
81.3
4.84
81.9
1.92
84.3
5.68
Spleen
95.1
6.40
95.7
7.61
90.9
3.33
81.0
3.11
83.1
6.19
91.9
2.00
Lung
94.3
9.37
99.8
1.38
92.3
5.74
96.5
7.32
89.0
1.32
90.0
3.05
Kidney
94.9
10.88
97.1
4.08
95.5
7.42
92.2
1.73
95.3
1.26
91.4
0.81
Uterus
88.2
10.51
96.5
2.33
99.9
5.11
94.3
9.57
99.2
2.72
96.5
7.75
Ovary
93.8
3.76
94.7
6.11
93.9
5.63
96.8
3.41
98.4
4.09
98.4
7.47
Muscle
97.3
5.29
89.2
0.90
91.9
2.68
92.6
5.77
98.2
4.76
97.6
3.20


## 3.1.5. Stability

The stability of processed samples which were kept in an autosampler for 24 h is shown in Table1. The accuracy ranged from 88.4% to 99.8%, and corresponding RSD ranged from 2.55% to 12.8%. The data were well within the acceptable limit. The results demonstrated that the four compounds were stable under the detected conditions.


## 3.2. Tissue Distribution Studies

The tissue distributions of the four coumarins were investigated at 4, 6, and 8 h after given orally 6.0 g/kg APR extract to female rats. The examined tissues included the heart, liver, spleen, lung, kidney, uterus, ovary, and muscle. The concentrations of four coumarins in tissues are presented in Table3. The mean concentration of four analytes at different tissues and points-in-time are shown in Figure 4. The highest and lowest concentrations of four coumarins were observed at 6 h and at 8 h in different tissues from 4 h to 8 h, respectively. The phenomenon indicated that the distribution tendency of four coumarins rose first then fell in most tissues. The reason that the highest concentration of four coumarins was detected in the liver at 6 h after oral administration APR extract was related to the liver in which the drugs metabolism mainly takes place. The concentration of columbianetin was higher than those of other constituents in muscle, which implied that columbianetin might be the main activity constituent in APR to cure arthrophlogosis. The relative higher distribution in kidney suggested that it might be the primary excretion tissue of the columbianetin. On the whole, the concentration of columbianetin was higher than other compounds, and the concentration order of four coumarins was columbianetin>osthole>columbianetin acetate > columbianadin in eight tissues, successively. The concentration of columbianetin acetate and osthole in uterus and ovary was higher than those in other tissues, which probably implied that both components exert estrogen-like effect in uterus and ovary. This result deeply proved the effect of estrogen-like activity of osthole in MCF-7 cells.Table 3
The concentrations of columbianetin, columbianetin acetate, osthole, and columbianadin in tissues (ng/mL)(n=6).
Tissues
columbianetin
columbianetin acetate
osthole
columbianadin
4h
6h
8h
4h
6h
8h
4h
6h
8h
4h
6h
8h
Heart
735.0±316.5
814.6±192.3
86.9±80.2
77.4±41.2
175.0±50.0
45.3±40.7
183.5±56.0
227.4±39.3
135.5±28.6
0.00±0.00
54.3±36.8
0.0±0.0
Liver
1467.0±913.4
2032±514
325.4±262.5
183.3±22.2
1677.0±42.4
94.0±86.4
669.3±286.5
1352±529.0
257.8±24.3
273.2±185.4
908.4±738.6
46.8±51.3
Spleen
533.1±266.9
529.1±243.6
171.0±9.9
47.4±30.6
180.9±0.0
42.8±53.4
191.2±48.5
206.6±67.6
153.5±42.6
0.00±0.00
0.00±0.00
12.2±24.4
Lung
653.0±306.1
647.4±227.5
95.3±72.0
76.6±46.9
94.5±70.9
16.9±17.7
275.0±101.6
188.3±49.0
125.8±24.7
0.00±0.00
0.00±0.00
0.0±0.0
Kidney
810.3±460.5
933.7±295.8
222.1±41.5
104.7±45.7
180.9±0.0
50.5±40.9
221.2±86.1
264.1±19.6
162.5±38.2
136.3±60.9
0.00±0.00
142.2±47.4
Uterus
565.1±129.8
582.6±130.5
155.1±34.8
140.5±49.2
428.3±47.0
77.7±76.9
261.0±57.0
382.6±83.5
167.0±42.3
133.7±44.6
0.00±0.00
0.00±0.00
Ovary
614.6±341.2
604.9±132.2
91.4±16.3
204.8±42.6
292.9±145.0
98.6±38.8
358.5±78.3
403.5±97.6
224.0±20.2
118.7±68.5
126.0±9.7
0.00±0.00
Muscle
712.2±176.4
663.3±239.3
117.4 ±92.8
55.0±10.7
79.7±49.4
32.9±31.7
154.7±9.7
118.1±82.9
126.4±7.0
0.00±0.00
0.0±0.0
0.00±0.00Figure 4
The tendency graph of columbianetin, columbianetin acetate, osthole, and columbianadin in eight tissues at 4, 6, and 8 h after oral administration of APR extract (n=6).


## 4. Conclusion

A sensitive, reliable, and fast method was established and validated for the simultaneous tissue distribution of osthole, columbianetin, columbianetin acetate, and columbianadin after oral administration of APR extract in rat. The tissue distribution provides a process to understand the pharmacological activity of APRin vivo. The results showed that the four coumarins were mainly distributed in liver which is the drugs metabolism place. The main distributed ingredients are columbianetin and osthole; next are columbianetin acetate and columbianadin in various tissues. The results potentially imply that columbianetin and osthole are the active constituents in APR for curing rheumatoid arthritis. The tissues distribution of columbianetin, columbianetin acetate, osthole, and columbianadin would be meaningful for study of Angelicae pubescentis radix on the clinical application.



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*Source: 2365697-2019-02-07.xml*