# A New Generation of Glycoconjugated Azo Dyes Based on Aminosugars

Authors: Lorenzo Guazzelli; Giorgio Catelani; Felicia D’Andrea
Journal: International Journal of Carbohydrate Chemistry
 (2015)

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## Abstract

The third generation of glycoconjugated azo dyes (GADs) was prepared linking monoazo dyes to 6-amino-6-deoxy-d-galactose or 6′amino-6′-deoxylactose through mixed amido-ester connections. The complementary conjugation reactions were studied using the succinyl derivative of either the acetal protected aminosugar or the azo dye. Target “naturalized” GADs were obtained after acid hydrolysis of the acetal protecting groups present on the sugar moiety.


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## Body

## 1. Introduction

The most commonly employed class of industrial textile dyes are the so-called “disperse dyes” family, characterised by their extremely low solubility in water. These, in a finely dispersed state, are used in dyeing processes of synthetic fibres, such as polyester, polyacetate, and polyamide. Owing to the absence of ionic groups in these synthetic fibres, only apolar dyes can be utilized [1–3]. Among the various troublesome aspects related to the use of disperse dyes and their dyeing processes, the highest concerns are raised by (a) the use of substantial amounts of dispersing agents, which are needed to bring the insoluble dyes in a stable colloidal dispersion throughout the application process, and (b) the use of high temperatures, typically around 130°C, which demand large amounts of energy and require appropriate dyeing machines able to operate under pressure [1–3]. Azo and anthraquinone dyes represent the two most relevant types of disperse dyes. Although disperse dyes can differ considerably in molecular weight, only those with a low mass (typically between 200 and 500 Da) [1] are effectively used in the dyeing of textile apolar synthetic fibres.Recently, an innovative class of azo dyes [4–6] has been proposed. These have been obtained through the glycoconjugation of common mono- and disaccharides, such asd-glucose,d-galactose, and lactose, with some model synthetic monoazo dyes. Glycoconjugated azo dyes (GADs) have been prepared by the so-called “naturalisation” procedure, which is to link the azo dye component and the sugar component through difunctional spacers by means of either two consecutive esterification reactions or two consecutive alkylation reactions. Glycoconjugated azo dyes (GADs, Figure 1) type1 [4, 5] and type2 [6] characterised, respectively, by diester and diether bonds have been evaluated for their tinctorial properties, giving unexpected results. In fact, GADs dye synthetic fibres quickly in boiling water, at ambient pressure, and without the need of dispersant, thanks to the increased hydrophilic characteristics. Uniquely, GADs can also be used effectively to dye natural fibres, such as wool and silk, allowing the study of solutions to novel dyeing technologies. Moreover, biodegradation and detoxification of a selected di-estereal GAD byFusarium oxysporum gave promising results [7] for the treatment of the aqueous waste, representing a step further towards environmentally friendly tinctorial processes.Figure 1
General structure of glycoconjugated azo dyes (GADs).In a more recent investigation [8] high molecular weight anthraquinone or bis-azo disperse dyes only become water-soluble after conjugation with two lactose units. The double-conjugation procedure required a preliminary insertion of a malonate spacer followed by condensation with two 6′-amino-6′-deoxylactose units [8]. Also, glutamic acid was used as the additional spacer in order to allow the double-conjugation [9]. Presented here is an extension of our previous results, namely, the synthesis of the third generation of naturalized GADs by conjugation of low molecular weight monoazo dyes with either 6-amino-6-deoxy-d-galactose or 6′-amino-6′-deoxylactose through mixed amido-ester connections (3).


## 2. Experimental

Optical rotations were measured with a Perkin-Elmer 241 polarimeter at the sodium D-line (589 nm) at20±2°C using a 1 dm cell. 1H NMR and 13C NMR spectra were recorded with a Bruker AC 200 operating at 200.13 (1H) and 50.3 MHz (13C) or with a Bruker Avance II 250 spectrometer operating at 250.12 (1H) and 62.9 MHz (13C). Spin resonances were reported as chemical shifts (δ) in part per million (ppm) and the references to the residual peak of the solvent employed as follows: CDCl3 7.27 ppm (1H NMR) and 77.0 ppm (13C NMR, central band), CD3CN 1.94 ppm (1H NMR, central band) and 1.28 ppm (13C NMR, central band), and CD3OD 3.31 ppm (1H NMR, central band) and 49.0 ppm (13C NMR, central band). Coupling constants J were reported in Hertz (Hz). The assignments were made, when possible, with the aid of DEPT, HETCOR, COSY, and HSQC experiments and in the case of anomeric mixtures, referring to the differences in the peak intensities and comparison with values for known analogous compounds. UV-Vis spectra were recorded on a Cary-300 Agilent Technologies Cary Series UV-Vis Spectrophotometer. Elemental analyses were performed by Carlo Erba elemental analyzer MOD 1106 instrument. All reactions were followed by TLC on Kieselgel 60 F254 with detection by UV light and/or with ethanolic 10% phosphomolybdic or sulfuric acid and heating. Kieselgel 60 (E. Merck, 70–230 and 230–400 mesh, resp.) was used for column and flash chromatography. Solvents were dried and purified by distillation according to standard procedure [10] and stored over 4 Å molecular sieves activated for at least 24 h at 200°C. Na2SO4 or MgSO4 was used as the drying agent for solutions. 6-Amino-6-deoxy-1,2:3,4-di-O-isopropylidene-α-d-galactopyranose (4) [11], 6-amino-6-deoxy-3,4-O-isopropylidene-β-d-galactopyranosyl-(1→4)-2,3:5,6-di-O-isopropylidene-aldehydo-d-glucose dimethyl acetal (5) [8], 2-[ethyl-(4-phenyldiazenyl)-phenyl-amino]-ethanol (8) [12], and 4-[2-ethyl-(4-[(4-nitrophenyl)diazenyl]phenylamino)ethoxy]-4-oxobutanoic acid (10) [4] were prepared through literature methods.


### 2.1. 6-N-(3-Carboxy-propanoyl)-6-deoxy-1,2:3,4-di-O-isopropylidene-α-d-galactopyranose (6)

A solution of4 [11] (2.60 g, 10.0 mmol) in MeOH (70 mL) was treated with succinic anhydride (1.20 g, 12.0 mmol, 1.2 eq) and stirred at room temperature until the starting material was completely disappeared (TLC, 9:1 EtOAc-PrOHi). After 2 h, Et3N (3 mL) was added to the reaction mixture, which was stirred for 10 min at room temperature and then was repeatedly coevaporated with toluene (5 × 20 mL) at diminished pressure. The resulting residue was dissolved into CH2Cl2 (50 mL), neutralized with AcOH (6 mL), and washed with water (25 mL) and the aqueous phase was extracted with CH2Cl2 (4 × 30 mL). The combined organic layers were dried (MgSO4), filtered, and concentrated under diminished pressure. The crude residue (3.42 g) was subjected to flash chromatographic purification on silica gel (EtOAc) to give pure6 as white foam (2.75 g, 76% yield); αD  −9.8 (c 1.1, CHCl3); Rf 0.50 (9:1 EtOAc-PrOHi); 1H NMR (200.13 MHz, CDCl3): δ 1.29, 1.31, 1.38, 1.43 [4s, each 3H, 2 × C(CH3)3]; 2.41–2.55 [m, 4H, (CH2)2], 3.16 (ddd, 1H, J6a,6b 13.7 Hz, J6a,NH 5.3 Hz, J5,6a 8.0 Hz, H-6a), 3.42 (ddd, 1H, J6b,NH 6.4 Hz, J5,6b 4.7 Hz, H-6b), 3.86 (ddd, 1H, H-5), 4.19 (dd, 1H, J3,4 7.9 Hz, J4,5 1.8 Hz, H-4), 4.31 (dd, 1H, J1,2 5.0 Hz, J2,3 2.4 Hz, H-2), 4.59 (dd, 1H, H-3), 5.43 (d, 1H, H-1), 6.80 (bt, 1H, NH); 13C NMR (50.3 MHz, CDCl3): δ 24.6, 25.2, 26.2, 26.3 [2 × C(CH3)3]; 30.2, 31.0 [(CH2)2], 40.8 (C-6), 66.9 (C-5), 71.4, 71.6, 72.2, (C-2, C-3, C-4), 97.1 (C-1), 109.4, 109.8 [2 × C(CH3)3], 173.8, 174.6 (2 × C=O), Anal. Calcd for C16H25NO8 (359.37): C, 53.47; H, 7.01; N, 3.90. Found: C, 53.44; H, 6.99; N, 3.88.


### 2.2. 6-N-(3-Carboxy-propanoyl)-6-deoxy-3,4-O-isopropylidene-β-d-galactopyranosyl-(1→4)-2,3:5,6-di-O-isopropylidene-aldehydo-d-glucose Dimethyl Acetal (7)

A solution of amine5 [8] (1.00 g, 1.97 mmol) and succinic anhydride (216 mg, 2.16 mmol, 1.2 eq) in MeOH (40 mL) was allowed to react in the reaction conditions described for the preparation of6, leading to the residue (1.17 g) constituted exclusively (13C NMR) by7 (98% yield). An analytical sample of7 was obtained through flash chromatography over silica gel eluting with 9:1 EtOAc-PrOHi. Compound7 was a white foam; αD  +53.2 (c 1.1, CHCl3); Rf 0.51 (9:1 EtOAc-PrOHi); 1H NMR (250.12 MHz, CD3CN): δ 1.29, 1.34 [2s, each 6H, 2 × C(CH3)3], 1.39, 1.43 [2s, each 3H, C(CH3)3], 2.51 [m, 4H, (CH2)2], 3.44, 3.49 (2s, each 3H, 2 × OCH3), 3.13 (ddd, 1H, J5′,6′a 6.8 Hz, J6′a,NH 4.9 Hz, J6′a,6′b 13.8 Hz, H-6′a), 3.33 (dd, 1H, J1′,2′ 8.1 Hz, J2′,3′ 7.3 Hz, H-2′), 3.67–3.80 (m, 2H, H-5′, H-6′b), 3.84 (dd, 1H, J3,4 1.3 Hz, J4,5 5.4 Hz, H-4), 3.99 (dd, 1H, J3′,4′ 3.2 Hz, H-3′), 4.06 (m, 3H, H-3, H-6a, OH-2′), 4.09 (dd, 1H, J4′,5′ 5.1 Hz, H-4′), 4.22 (m, 2H, H-5, H-6b), 4.33 (d, 1H, H-1′), 4.36 (d, 1H, J1,2 6.4 Hz, H-1), 4.41 (dd, 1H, J2,3 7.1 Hz, H-2), 6.85 (bt, 1H, NH); 13C NMR (62.9 MHz, CD3CN): δ 25.2, 26.5, 26.6, 26.9, 27.4, 28.4 [3 × C(CH3)3], 30.1, 31.0 [(CH2)2], 58.1, 56.0 (2 × OCH3), 41.1 (C-6′), 66.1 (C-6), 74.6 (C-2′), 72.5 (C-5′), 75.0 (C-4′), 77.4 (C-2), 77.6 (C-5), 77.7 (C-4), 78.4 (C-3), 80.0 (C-3′), 104.0 (C-1′), 108.2 (C-1), 109.3, 110.4, 110.7 [3 × C(CH3)3], 173.6, 174.7 (2 × C=O). Anal. Calcd for C27H45NO14 (607.28): C, 53.37; H, 7.46; N, 2.31. Found: C, 53.35; H, 7.43; N, 2.29.


### 2.3. Preparation of Protected GAD11

A solution of6 (1.50 g, 4.17 mmol) and azo dye9 (1.81 g, 5.17 mmol, 1.24 eq) in dry THF (50 mL) was treated withN,N′-dicyclohexylcarbodiimide (DDC) (1.03 g, 5.0 mmol, 1.2 eq) and 4-dimethylaminopyridine (DMAP) (145 mg, 1.19 mmol) and the solution was stirred at room temperature until the starting material was completely disappeared (4 h, TLC, EtOAc). The reaction mixture was concentrated under diminished pressure and the crude residue was dissolved into CH2Cl2 (70 mL) and washed in the order with saturated aqueous NaHCO3 (3 × 30 mL) and brine (30 mL). The organic phase was dried (Na2SO4), filtered, and concentrated under diminished pressure. Purification of the crude residue (3.10 g) by flash chromatography over silica gel (2:3 hexane-EtOAc) gave pure11 as a red foam (2.14 g, 74% yield); Rf 0.22 (2:3 hexane-EtOAc); 1H NMR (200.13 MHz, CDCl3): δ 1.27 (t, 3H,J 7.2 Hz, CH3), 1.32, 1.33, 1.45, 1.48, [4s, each 3H, 2 × C(CH3)3], 2.65, 2.48 [2m, each 2H, (CH2)2], 3.20 (ddd, 1H, J6a,6b 13.9 Hz, J5,6a 8.9 Hz, J6a,NH 3.7 Hz, H-6a), 3.55 (q, 2H,J 7.2 Hz, CH3CH2N), 3.70 (t, 2H,J 6.2 Hz, OCH2CH2N), 3.72 (ddd, 1H, J5,6b 3.5 Hz, J6b,NH 7.2 Hz, H-6b), 3.87 (ddd, 1H, H-5), 4.19 (dd, 1H, J4,5 1.7 Hz, J3,4 7.9 Hz, H-4), 4.31 (dd, 1H, J1,2 5.0 Hz, J2,3 2.5 Hz, H-2), 4.32 (t, 2H,J 6.2 Hz, CH2OCO), 4.59 (dd, 1H, H-3), 5.51 (d, 1H, H-1), 5.98 (dd, 1H, NH), 7.79 (d, 1H, Ar-H-6), 6.87, 7.96 (AA′XX′ system, 4H, Ar-H-8, Ar-H-9, Ar-H-11, Ar-H-12), 8.16 (dd, 1H, J5,6 9.0 Hz, Ar-H-5), 8.40 (d, 1H, J3,5 2.4 Hz, Ar-H-3); 13C NMR (50.3 MHz, CDCl3): δ 12.2 (CH3), 24.3, 24.9, 25.9, 26.0 [2 × C(CH3)3], 29.3, 30.6 [(CH2)2], 40.0 (C-6), 45.8 (CH3CH2N), 48.8 (OCH2CH2N), 61.3 (CH2OCO), 66.3 (C-5), 70.5 (C-2), 70.6 (C-3), 71.6 (C-4), 96.1 (C-1), 108.7, 109.4 [2 × C(CH3)3], 111.5, 111.6, 117.9, 122.5, 125.9, 126.9, 126.9 (7 × Ar-CH), 133.9 (Ar-C-2), 144.4 (Ar-C-7), 147.1 (Ar-C-4), 151.6, 153.0 (Ar-C-1, Ar-C-10), 171.3, 172.7 (2 × C=O). Anal. Calcd for C32H40ClN5O10 (690.14): C, 55.69; H, 5.84; N, 10.15. Found: C, 55.66; H, 5.83; N, 10.12.


### 2.4. Preparation of Protected GAD12

The condensation of7 (1.50 g, 2.47 mmol) and azo dye9 (1.06 g, 3.05 mmol, 1.24 eq) withN,N′-dicyclohexylcarbodiimide (DDC) (630 mg, 3.05 mmol) and 4-dimethylaminopyridine (DMAP) (90 mg, 0.73 mmol) in dry THF (30 mL) was performed according to the procedure described above for11. The flash chromatography over silica gel of the crude product (1:4 hexane-EtOAc) led to pure12 (1.90 g, 82% yield) as a red foam; Rf 0.27 (1:4 hexane-EtOAc); λmax 493.00 nm (MeOH); 1H NMR (250.12 MHz, CD3CN): δ 1.20 (t, 3H,J 7.1 Hz, CH3), 1.28, 1.33 [2s, each 6H, 2 × C(CH3)3], 1.37, 1.42 [2s, each 3H, C(CH3)3], 2.44, 2.55 [2m, each 2H, (CH2)2], 3.43, 3.47 (2s, each 3H, 2 × OCH3), 3.09 (ddd, 1H, J5′,6′a 8.1 Hz, J6′a,NH 5.5 Hz, J6′a,6′b 13.8 Hz, H-6′a), 3.30–3.41 (m, 2H, H-2′, OH-2′), 3.52 (q, 2H,J 7.1 Hz, CH3CH2N), 3.60–3.78 (m, 4H, OCH2CH2N, H-5′, H-6′b), 3.82 (dd, 1H, J3,4 1.2 Hz, J4,5 4.8 Hz, H-4), 3.90–4.12 (m, 5H, H-3′, H-4′, H-3, H-6a, H-6b), 4.18 (m, 1H, H-5), 4.25 (t, 2H,J 6.1 Hz, CH2OCO), 4.30 (d, 1H, J1′,2′ 8.2 Hz, H-1′), 4.34 (d, 1H, J1,2 6.5 Hz, H-1), 4.40 (dd, 1H, J2,3 7.1 Hz, H-2), 6.67 (bt, 1H, NH), 7.73 (d, 1H, Ar-H-6), 6.86, 7.85 (AA′XX′ system, 4H, Ar-H-8, Ar-H-9, Ar-H-11, Ar-H-12), 8.15 (dd, 1H, J5,6 9.0 Hz, Ar-H-5), 8.37 (d, 1H, J3,5 2.4 Hz, Ar-H-3); 13C NMR (62.9 MHz, CD3CN): δ 12.4 (CH3), 25.1, 26.5, 26.6, 26.9, 27.4, 28.4 [3 × C(CH3)3], 29.1, 30.9 [(CH2)2], 40.9 (C-6′), 46.4 (CH3CH2N), 49.5 (OCH2CH2N), 56.0, 58.0 (2 × OCH3), 66.1 (C-6), 62.3 (CH2OCO), 74.6 (C-2′), 72.7 (C-5′), 75.0 (C-4′), 77.4 (C-2), 77.6 (C-5), 77.8 (C-4), 78.4 (C-3), 80.1 (C-3′), 104.0 (C-1′), 108.2 (C-1), 109.4, 110.6, 110.7 [3 × C(CH3)3], 112.7, 112.7, 118.8, 123.9, 126.8, 127.6, 127.7 (7 × Ar-CH), 134.1 (Ar-C-2), 144.8 (Ar-C-7), 148.2 (Ar-C-4), 153.3, 153.8 (Ar-C-1, Ar-C-10), 172.3, 173.7 (2 × C=O). Anal. Calcd for C43H60ClN5O16 (938.41): C, 55.04; H, 6.44; N, 7.46. Found: C, 55.02; H, 6.42; N, 7.43.


### 2.5. Preparation of Protected GAD13

A solution of6 (1.0 g, 2.78 mmol), azo dye8 (950 mg, 3.52 mmol, 1.25 eq),N,N′-dicyclohexylcarbodiimide (DDC) (688 mg, 3.34 mmol), and 4-dimethylaminopyridine (DMAP) (102 mg, 0.83 mmol) in dry THF (30 mL) was allowed to react in the reaction conditions described for the preparation of11. The flash chromatography over silica gel (1:1 hexane-EtOAc) afforded pure13 (1.47 g, 86% yield) as a yellow foam; Rf 0.31 (4:6 hexane-EtOAc); λmax 407.00 nm (MeOH); 1H NMR (200.13 MHz, CDCl3): δ 1.22 (t, 3H,J 7.0 Hz, CH3), 1.30, 1.33, 1.44, 1.48 [4s, each 3H, 2 × C(CH3)3], 2.47, 2.66 [2m, each 2H, (CH2)2], 3.20 (ddd, 1H, J6a,6b 14.0 Hz, J5,6a 9.0 Hz, J6a,NH 3.8 Hz, H-6a), 3.49 (q, 2H,J 7.0 Hz, CH3CH2N), 3.64 (t, 2H,J 6.2 Hz, OCH2CH2N), 3.71 (ddd, 1H, J5,6b 3.5 Hz, J6b,NH 7.7 Hz, H-6b), 3.87 (ddd, 1H, H-5), 4.18 (dd, 1H, J3,4 7.9 Hz, J4,5 1.8 Hz, H-4), 4.30 (dd, 1H, J1,2 5.0 Hz, J2,3 2.4 Hz, H-2), 4.28 (t, 2H,J 6.2 Hz, CH2OCO), 4.57 (dd, 1H, H-3), 5.50 (d, 1H, H-1), 6.00 (dd, 1H, NH), 6.78 (m, 2H, Ar-H-9, Ar-H-11), 7.37–7.51 (m, 3H, Ar-H-3, Ar-H-4, Ar-H-5), 7.81–7.90 (m, 4H, Ar-H-2, Ar-H-6, Ar-H-8, Ar-H-12); 13C NMR (50.3 MHz, CDCl3): δ 12.2 (CH3), 24.3, 24.9, 25.8, 25.9 [2 × C(CH3)3], 29.3, 30.7 [(CH2)2], 40.0 (C-6), 45.5 (CH3CH2N), 48.7 (OCH2CH2N), 61.5 (CH2OCO), 66.3 (C-5), 70.5 (C-2), 70.7 (C-3), 71.6 (C-4), 96.2 (C-1), 108.7, 109.4 [2 × C(CH3)3], 111.3, 122.1, 125.2, 126.4, 128.9 (Ar-CH), 143.6 (Ar-C-7), 149.9, 153.0, (Ar-C-1, Ar-C-10), 171.4, 172.7 (2 × C=O). Anal. Calcd for C32H42N4O8 (610.70): C, 62.94; H, 6.93; N, 9.17. Found: C, 62.91; H, 6.90; N, 9.15.


### 2.6. Preparation of Protected GAD14

A solution of azo dye10 (152 mg, 0.367 mmol) and amine4 (114 mg, 0.441 mmol, 1.2 eq) in dry THF (4 mL) was stirred at room temperature (10 min). 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM) [13] (122 mg, 0.441 mmol, 1.2 eq) was added and the mixture was stirred at room temperature until azo dye10 was completely disappeared (20 h, TLC, EtOAc). The reaction mixture was concentrated under diminished pressure and the crude residue was partitioned between water (10 mL) and Et2O (10 mL) and the aqueous phase extracted with Et2O (3 × 10 mL). The collected organic phases were dried (Na2SO4), filtered, and concentrated under diminished pressure. Purification of the crude residue (3.10 g) by flash chromatography over silica gel (1:4 hexane-EtOAc) gave pure14 as a red foam solid (230 mg, 95% yield calculated from10); Rf 0.45 (EtOAc); 1H NMR (250.12 MHz, CD3CN): δ 1.19 (t, 3H,J 7.0 Hz, CH3), 1.26, 1.29, 1.37, 1.41 [4s, each 3H, 2 × C(CH3)3], 2.38, 2.52 [2m, each 2H, (CH2)2], 3.12 (ddd, 1H, J6a,6b 13.7 Hz, J5,6a 8.1 Hz, J6a,NH 5.5 Hz, H-6a), 3.36 (ddd, 1H, J5,6b 5.2 Hz, J6b,NH 6.4 Hz, H-6b), 3.54 (q, 2H,J 7.0 Hz, CH3CH2N), 3.68 (t, 2H,J 5.9 Hz, CH2CH2N), 3.84 (ddd, 1H, H-5), 4.17 (dd, 1H, J3,4 7.9 Hz, J4,5 1.9 Hz, H-4), 4.29 (dd, 1H, J1,2 4.9 Hz, J2,3 2.5 Hz, H-2), 4.26 (t, 2H,J 5.9 Hz, CH2OCO), 4.57 (dd, 1H, H-3), 5.41 (d, 1H, H-1), 6.52 (bt, 1H, NH), 6.89, 7.86 (AA′XX′ system, 4H, Ar-H-8, Ar-H-9, Ar-H-11, Ar-H-12), 7.91, 8.31 (AA′XX′ system, 4H, Ar-H-2, Ar-H-3, Ar-H-5, Ar-H-6); 13C NMR (62.9 MHz, CD3CN): δ 12.4 (CH3), 24.5, 25.2, 26.2, 26.3 [2 × C(CH3)3], 30.0, 30.9 [(CH2)2], 40.6 (C-6), 46.3 (CH3CH2N), 49.4 (CH2CH2N), 62.3 (CH2OCO), 66.9 (C-5), 71.4 (C-2), 71.5 (C-3), 72.2 (C-4), 96.1 (C-1), 109.3, 109.8 [2 × C(CH3)3], 112.6, 123.3, 125.7, 126.9 (8 × Ar-CH), 144.3 (Ar-C-7), 148.4 (Ar-C-4), 152.7, 157.7 (Ar-C-1, Ar-C-10), 172.4, 173.6 (2 × C=O). Anal. Calcd for C32H41N5O10 (655.70): C, 58.62; H, 6.30; N, 10.68. Found: C, 58.60; H, 6.28; N, 10.65.


### 2.7. Preparation of Protected GAD15

The condensation of azo dye10 (151 mg, 0.367 mmol) and amine5 (224 mg, 0.441 mmol, 1.2 eq) with 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM) [13] (122 mg, 0441 mmol, 1.2 eq) in dry THF (4 mL) was performed according to the procedure described above for the preparation of14. The flash chromatography over silica gel (1:9 hexane-EtOAc) of the crude reaction product led to pure15 (252 mg, 76% yield calculated from10) as a red foam; Rf 0.32 (EtOAc); 1H NMR (250.12 MHz, CD3CN): δ 1.18 (t, 3H,J 7.0 Hz, CH3), 1.27, 1.33 [2s, each 6H, 2 × C(CH3)3], 1.37, 1.42 [2s, each 3H, C(CH3)3], 2.44, 2.55 [2m, each 2H, (CH2)2], 3.43, 3.46 (2s, each 3H, 2 × OCH3), 3.11 (ddd, 1H, J5′,6′a 8.8 Hz, J6′a,NH 5.2 Hz, J6′a,6′b 13.4 Hz, H-6′a), 3.32 (dd, 1H, J1′,2′ 8.0 Hz, J2′,3′ 7.3 Hz, H-2′), 3.51 (q, 2H,J 7.0 Hz, CH3CH2N), 3.67 (m, 2H,J 6.0 Hz, CH2CH2N), 3.70–3.75 (m, 3H, H-5′, H-6′b, OH-2′), 3.82 (dd, 1H, J4,5 5.2 Hz, J3,4 1.3 Hz, H-4), 3.94 (dd, 1H, J3′,4′ 3.4 Hz, H-3′), 4.00 (dd, 1H, J6a,6b 8.1 Hz, J5,6a 6.3 Hz, H-6a), 4.03 (dd, 1H, J2,3 7.2 Hz, H-3), 4.04–4.10 (m, 2H, H-4′, H-6b), 4.22 (dt, 1H, J5,6a=J5,6b 6.3 Hz, H-5), 4.27 (t, 2H,J 6.0 Hz, CH2OCO), 4.31 (d, 1H, H-1′), 4.35 (d, 1H, J1,2 6.4 Hz, H-1), 4.41 (dd, 1H, H-2), 6.68 (bt, 1H, NH), 6.85, 7.68 (AA′XX′ system, 4H, Ar-H-8, Ar-H-9, Ar-H-11, Ar-H-12), 7.88, 8.29 (AA′XX′ system, 4H, Ar-H-2, Ar-H-3, Ar-H-5, Ar-H-6); 13C NMR (62.9 MHz, CD3CN): δ 12.4 (CH3), 25.1, 26.4, 26.5, 26.9, 27.4, 28.4 [3 × C(CH3)3], 30.9, 29.9 [(CH2)2], 40.9 (C-6′), 46.2 (CH3CH2N), 49.5 (OCH2CH2N), 56.0, 58.0 (2 × OCH3), 62.3 (CH2OCO), 66.0 (C-6), 74.6 (C-2′), 72.6 (C-5′), 75.0 (C-4′), 77.4 (C-2), 77.6 (C-5), 77.7 (C-4), 78.4 (C-3), 80.0 (C-3′), 104.1 (C-1′), 108.2 (C-1), 109.2, 110.4, 110.6 [3 × C(CH3)3], 112.6, 123.3, 125.7, 126.9 (8 × Ar-CH), 144.2 (Ar-C-7), 148.3 (Ar-C-4), 152.7, 157.6 (Ar-C-1, Ar-C-10), 172.3, 173.7 (2 × C=O). Anal. Calcd for C43H61N5O16 (903.37): C, 57.13; H, 6.80; N, 7.75. Found: C, 57.11; H, 6.77; N, 7.72.


### 2.8. Preparation of Deprotected GAD16

A solution of11 (1.07 g, 1.55 mmol) in 90% aqueous CF3COOH (70 mL) was stirred at room temperature until the starting material was completely disappeared (3 h, TLC, EtOAc). The red-violet solution was concentrated under diminished pressure and repeatedly coevaporated with toluene (5 × 30 mL). The crude residue was dissolved in EtOAc (100 mL) and neutralized with saturated aqueous NaHCO3 (20 mL). The aqueous phase was extracted with EtOAc (3 × 50 mL) and the collected layers were dried, filtered, and concentrated under diminished pressure. The residue was triturated with Et2O and the red residue was constituted (NMR) only by the azo dye16 (640 mg, 98% yield) as a mixture of α- and β-pyranose anomers (NMR) in a ratio of 3 : 2, calculated on the basis of the relative C-1 signal intensities. Compound16 was a red solid foam, Rf 0.21 (9:1 EtOAc-MeOH); Selected 1H NMR (200.13 MHz, CD3OD) signals for both anomers: δ 1.06 (t, 3H,J 6.7 Hz, CH3), 2.48–2.30 [m, 4H, (CH2)2], 6.73, 7.64, (AA′XX′ system, 4H, Ar-H-8, Ar-H-9, Ar-H-11, Ar-H-12), 7.54 (bd, 1H, Ar-H-6), 8.00 (dd, 1H, J5,6 9.0 Hz, Ar-H-5), 8.40 (d, 1H, J3,5 2.2 Hz, Ar-H-3); 13C NMR (50.3 MHz, CD3OD): δα-pyranose 40.8 (C-6), 68.8 (C-5), 69.2 (C-4), 69.7 (C-2, C-3), 93.8 (C-1), β-pyranose 40.7 (C-6), 69.1 (C-4), 72.5 (C-3), 73.0, 73.7 (C-2, C-5), 97.8 (C-1), cluster of signals for both anomers: δ 12.5 (CH3), 29.4–30.9 [(CH2)2], 45.9 (CH3CH2N), 48.9 (OCH2CH2N), 62.0 (CH2OCO), 112.2–127.2 (Ar-CH), 133.0 (Ar-C-2), 143.9 (Ar-C-7), 147.0 (Ar-C-4), 152.6, 152.8 (Ar-C-1, Ar-C-10), 172.4, 173.2 (2 × C=O). Calcd for C26H32ClN5O10 (610.01): C, 51.19; H, 5.29; N, 11.48. Found: C, 51.17; H, 5.28; N, 11.45.


### 2.9. Preparation of Deprotected GAD17

Hydrolysis of12 (640 mg, 0.682 mmol) with 90% aqueous CF3COOH (8 mL) was performed as described above for the preparation of16. After 2 h of stirring, the red-violet solution was concentrated under diminished pressure, neutralized, and extracted as described above to give a red foam constituted (NMR) only by the azo dye17 (485 mg, 92% yield) as a mixture of α- and β-pyranose anomers (NMR) in the ratio of 3 : 2, calculated on the basis of the relative C-1 signal intensities. Compound17 was a red solid foam, Rf 0.18 (8:2 EtOAc-MeOH); λmax 493.25 nm (MeOH); Selected 1H NMR (200.13 MHz, CD3OD): δ 1.20 (t, 3H,J 7.1 Hz, CH3, α- and β-pyranose), 2.48, 2.57 [2m, each 2H, (CH2)2, α- and β-pyranose], 3.18 (dd, 1H, J1,2 7.8 Hz, J2,3 9.0 Hz, H-2, β-pyranose), 4.18 (d, 1H, J1′,2′ 8.0 Hz, H-1′, α-pyranose), 4.20 (d, 1H, J1′,2′ 7.8 Hz, H-1′, β-pyranose), 4.25 (bt, 2H,J 6.4 Hz, CH2OCO, α- and β-pyranose), 4.31 (bt, 2H,J 6.6 Hz, CH2OCO, α- and β-pyranose), 4.48 (d, 1H, H-1, β-pyranose), 4.49–4.61 (m, H-2′, α- and β-pyranose), 5.06 (d, 1H, J1,2 3.7 Hz, H-1, α-pyranose), 6.85, 7.83 (AA′XX′ system, 4H, Ar-H-8, Ar-H-9, Ar-H-11, Ar-H-12, α- and β-pyranose), 7.72 (bd, 1H, Ar-H-6, α- and β-pyranose), 8.13 (bd, 1H,J 9.1 Hz, Ar-H-5, α- and β-pyranose), 8.32 (bd, 1H, Ar-H-3, α- and β-pyranose); 13C NMR (62.9 MHz, CD3OD): δα-pyranose 41.3 (C-6′), 62.0 (C-6), 70.5 (C-4′), 71.4 (C-5), 72.1 (C-2′), 72.4 (C-2), 73.0 (C-3), 73.4 (C-3′), 76.0 (C-5′), 81.1 (C-4), 93.7 (C-1′), 105.0 (C-1), β-pyranose 41.2 (C-6′), 62.8 (C-6), 70.4 (C-4′), 72.3 (C-2′), 73.1 (C-3′), 74.5, 74.6 (C-3, C-2), 74.7 (C-5), 76.2 (C-5′), 80.5 (C-4), 98.1 (C-1′), 105.3 (C-1), cluster of signals for both anomers: δ 12.5 (CH3), 30.3, 31.3 [(CH2)2], 46.7 (CH3CH2N), 50.0 (OCH2CH2N), 62.9 (CH2OCO), 112.9–129.9 (7 × Ar-CH), 134.7 (Ar-C-2), 145.6 (Ar-C-7), 148.6 (Ar-C-4), 153.6, 154.3 (Ar-C-1, Ar-C-10), 174.4, 174.9 (2 × C=O). Anal. Calcd for C32H42ClN5O15 (772.15): C, 49.78; H, 5.48; N, 9.07. Found: C, 49.75; H, 5.46; N, 9.05.


### 2.10. Preparation of Deprotected GAD18

The hydrolysis of13 (193 mg, 0.316 mmol) with 90% aqueous CF3COOH (3 mL) was performed as described above for the preparation of16. After 2 h of stirring, the red-violet solution was concentrated under diminished pressure, neutralized (saturated aqueous NaHCO3) until the red-violet colour turned to yellow-orange, and extracted as described above to give a yellow foam constituted (NMR) only by the azo dye18 (160 mg, 95% yield). Compound18 was a mixture of α- and β-pyranose anomers (NMR) in the ratio of 1 : 1, calculated on the basis of the relative C-1 signal intensities; Rf 0.19 (9:1 EtOAc-MeOH); λmax 411.25 nm (MeOH); Selected 1H NMR (200.13 MHz, CD3OD) signals for both anomers: δ 1.21 (t, 3H,J 6.1 Hz, CH3), 2.58 [m, 4H, (CH2)2], 6.83 (m, 2H, Ar-H-9, Ar-H-11), 7.35–7.50 (m, 3H, Ar-H-3, Ar-H-4, Ar-H-5), 7.75–7.83 (m, 4H, Ar-H-2, Ar-H-6, Ar-H-8, Ar-H-12); 13C NMR (62.9 MHz, CD3OD): δα-pyranose 40.1 (C-6), 67.5 (C-5), 69.4, 69.5, 69.7 (C-2, C-3, C-4), 93.6 (C-1), β-pyranose 40.5 (C-6), 70.3 (C-4), 72.1 (C-3), 73.1, 74.1 (C-2, C-5), 98.2 (C-1), cluster of signals for both anomers: δ 11.9 (CH3), 28.5, 28.6 [(CH2)2], 46.2 (CH3CH2N), 52.9 (OCH2CH2N), 59.8 (CH2OCO), 111.1, 122.5, 125.7, 129.5, 129.9 (Ar-CH), 143.2 (Ar-C-7), 150.1, 153.0 (Ar-C-1, Ar-C-10), 174.0, 174.2 (2 × C=O). Anal. Calcd for C26H34N4O8 (530.57): C, 58.86; H, 6.46; N, 10.56. Found: C, 58.83; H, 6.42; N, 10.53.


## 2.1. 6-N-(3-Carboxy-propanoyl)-6-deoxy-1,2:3,4-di-O-isopropylidene-α-d-galactopyranose (6)

A solution of4 [11] (2.60 g, 10.0 mmol) in MeOH (70 mL) was treated with succinic anhydride (1.20 g, 12.0 mmol, 1.2 eq) and stirred at room temperature until the starting material was completely disappeared (TLC, 9:1 EtOAc-PrOHi). After 2 h, Et3N (3 mL) was added to the reaction mixture, which was stirred for 10 min at room temperature and then was repeatedly coevaporated with toluene (5 × 20 mL) at diminished pressure. The resulting residue was dissolved into CH2Cl2 (50 mL), neutralized with AcOH (6 mL), and washed with water (25 mL) and the aqueous phase was extracted with CH2Cl2 (4 × 30 mL). The combined organic layers were dried (MgSO4), filtered, and concentrated under diminished pressure. The crude residue (3.42 g) was subjected to flash chromatographic purification on silica gel (EtOAc) to give pure6 as white foam (2.75 g, 76% yield); αD  −9.8 (c 1.1, CHCl3); Rf 0.50 (9:1 EtOAc-PrOHi); 1H NMR (200.13 MHz, CDCl3): δ 1.29, 1.31, 1.38, 1.43 [4s, each 3H, 2 × C(CH3)3]; 2.41–2.55 [m, 4H, (CH2)2], 3.16 (ddd, 1H, J6a,6b 13.7 Hz, J6a,NH 5.3 Hz, J5,6a 8.0 Hz, H-6a), 3.42 (ddd, 1H, J6b,NH 6.4 Hz, J5,6b 4.7 Hz, H-6b), 3.86 (ddd, 1H, H-5), 4.19 (dd, 1H, J3,4 7.9 Hz, J4,5 1.8 Hz, H-4), 4.31 (dd, 1H, J1,2 5.0 Hz, J2,3 2.4 Hz, H-2), 4.59 (dd, 1H, H-3), 5.43 (d, 1H, H-1), 6.80 (bt, 1H, NH); 13C NMR (50.3 MHz, CDCl3): δ 24.6, 25.2, 26.2, 26.3 [2 × C(CH3)3]; 30.2, 31.0 [(CH2)2], 40.8 (C-6), 66.9 (C-5), 71.4, 71.6, 72.2, (C-2, C-3, C-4), 97.1 (C-1), 109.4, 109.8 [2 × C(CH3)3], 173.8, 174.6 (2 × C=O), Anal. Calcd for C16H25NO8 (359.37): C, 53.47; H, 7.01; N, 3.90. Found: C, 53.44; H, 6.99; N, 3.88.


## 2.2. 6-N-(3-Carboxy-propanoyl)-6-deoxy-3,4-O-isopropylidene-β-d-galactopyranosyl-(1→4)-2,3:5,6-di-O-isopropylidene-aldehydo-d-glucose Dimethyl Acetal (7)

A solution of amine5 [8] (1.00 g, 1.97 mmol) and succinic anhydride (216 mg, 2.16 mmol, 1.2 eq) in MeOH (40 mL) was allowed to react in the reaction conditions described for the preparation of6, leading to the residue (1.17 g) constituted exclusively (13C NMR) by7 (98% yield). An analytical sample of7 was obtained through flash chromatography over silica gel eluting with 9:1 EtOAc-PrOHi. Compound7 was a white foam; αD  +53.2 (c 1.1, CHCl3); Rf 0.51 (9:1 EtOAc-PrOHi); 1H NMR (250.12 MHz, CD3CN): δ 1.29, 1.34 [2s, each 6H, 2 × C(CH3)3], 1.39, 1.43 [2s, each 3H, C(CH3)3], 2.51 [m, 4H, (CH2)2], 3.44, 3.49 (2s, each 3H, 2 × OCH3), 3.13 (ddd, 1H, J5′,6′a 6.8 Hz, J6′a,NH 4.9 Hz, J6′a,6′b 13.8 Hz, H-6′a), 3.33 (dd, 1H, J1′,2′ 8.1 Hz, J2′,3′ 7.3 Hz, H-2′), 3.67–3.80 (m, 2H, H-5′, H-6′b), 3.84 (dd, 1H, J3,4 1.3 Hz, J4,5 5.4 Hz, H-4), 3.99 (dd, 1H, J3′,4′ 3.2 Hz, H-3′), 4.06 (m, 3H, H-3, H-6a, OH-2′), 4.09 (dd, 1H, J4′,5′ 5.1 Hz, H-4′), 4.22 (m, 2H, H-5, H-6b), 4.33 (d, 1H, H-1′), 4.36 (d, 1H, J1,2 6.4 Hz, H-1), 4.41 (dd, 1H, J2,3 7.1 Hz, H-2), 6.85 (bt, 1H, NH); 13C NMR (62.9 MHz, CD3CN): δ 25.2, 26.5, 26.6, 26.9, 27.4, 28.4 [3 × C(CH3)3], 30.1, 31.0 [(CH2)2], 58.1, 56.0 (2 × OCH3), 41.1 (C-6′), 66.1 (C-6), 74.6 (C-2′), 72.5 (C-5′), 75.0 (C-4′), 77.4 (C-2), 77.6 (C-5), 77.7 (C-4), 78.4 (C-3), 80.0 (C-3′), 104.0 (C-1′), 108.2 (C-1), 109.3, 110.4, 110.7 [3 × C(CH3)3], 173.6, 174.7 (2 × C=O). Anal. Calcd for C27H45NO14 (607.28): C, 53.37; H, 7.46; N, 2.31. Found: C, 53.35; H, 7.43; N, 2.29.


## 2.3. Preparation of Protected GAD11

A solution of6 (1.50 g, 4.17 mmol) and azo dye9 (1.81 g, 5.17 mmol, 1.24 eq) in dry THF (50 mL) was treated withN,N′-dicyclohexylcarbodiimide (DDC) (1.03 g, 5.0 mmol, 1.2 eq) and 4-dimethylaminopyridine (DMAP) (145 mg, 1.19 mmol) and the solution was stirred at room temperature until the starting material was completely disappeared (4 h, TLC, EtOAc). The reaction mixture was concentrated under diminished pressure and the crude residue was dissolved into CH2Cl2 (70 mL) and washed in the order with saturated aqueous NaHCO3 (3 × 30 mL) and brine (30 mL). The organic phase was dried (Na2SO4), filtered, and concentrated under diminished pressure. Purification of the crude residue (3.10 g) by flash chromatography over silica gel (2:3 hexane-EtOAc) gave pure11 as a red foam (2.14 g, 74% yield); Rf 0.22 (2:3 hexane-EtOAc); 1H NMR (200.13 MHz, CDCl3): δ 1.27 (t, 3H,J 7.2 Hz, CH3), 1.32, 1.33, 1.45, 1.48, [4s, each 3H, 2 × C(CH3)3], 2.65, 2.48 [2m, each 2H, (CH2)2], 3.20 (ddd, 1H, J6a,6b 13.9 Hz, J5,6a 8.9 Hz, J6a,NH 3.7 Hz, H-6a), 3.55 (q, 2H,J 7.2 Hz, CH3CH2N), 3.70 (t, 2H,J 6.2 Hz, OCH2CH2N), 3.72 (ddd, 1H, J5,6b 3.5 Hz, J6b,NH 7.2 Hz, H-6b), 3.87 (ddd, 1H, H-5), 4.19 (dd, 1H, J4,5 1.7 Hz, J3,4 7.9 Hz, H-4), 4.31 (dd, 1H, J1,2 5.0 Hz, J2,3 2.5 Hz, H-2), 4.32 (t, 2H,J 6.2 Hz, CH2OCO), 4.59 (dd, 1H, H-3), 5.51 (d, 1H, H-1), 5.98 (dd, 1H, NH), 7.79 (d, 1H, Ar-H-6), 6.87, 7.96 (AA′XX′ system, 4H, Ar-H-8, Ar-H-9, Ar-H-11, Ar-H-12), 8.16 (dd, 1H, J5,6 9.0 Hz, Ar-H-5), 8.40 (d, 1H, J3,5 2.4 Hz, Ar-H-3); 13C NMR (50.3 MHz, CDCl3): δ 12.2 (CH3), 24.3, 24.9, 25.9, 26.0 [2 × C(CH3)3], 29.3, 30.6 [(CH2)2], 40.0 (C-6), 45.8 (CH3CH2N), 48.8 (OCH2CH2N), 61.3 (CH2OCO), 66.3 (C-5), 70.5 (C-2), 70.6 (C-3), 71.6 (C-4), 96.1 (C-1), 108.7, 109.4 [2 × C(CH3)3], 111.5, 111.6, 117.9, 122.5, 125.9, 126.9, 126.9 (7 × Ar-CH), 133.9 (Ar-C-2), 144.4 (Ar-C-7), 147.1 (Ar-C-4), 151.6, 153.0 (Ar-C-1, Ar-C-10), 171.3, 172.7 (2 × C=O). Anal. Calcd for C32H40ClN5O10 (690.14): C, 55.69; H, 5.84; N, 10.15. Found: C, 55.66; H, 5.83; N, 10.12.


## 2.4. Preparation of Protected GAD12

The condensation of7 (1.50 g, 2.47 mmol) and azo dye9 (1.06 g, 3.05 mmol, 1.24 eq) withN,N′-dicyclohexylcarbodiimide (DDC) (630 mg, 3.05 mmol) and 4-dimethylaminopyridine (DMAP) (90 mg, 0.73 mmol) in dry THF (30 mL) was performed according to the procedure described above for11. The flash chromatography over silica gel of the crude product (1:4 hexane-EtOAc) led to pure12 (1.90 g, 82% yield) as a red foam; Rf 0.27 (1:4 hexane-EtOAc); λmax 493.00 nm (MeOH); 1H NMR (250.12 MHz, CD3CN): δ 1.20 (t, 3H,J 7.1 Hz, CH3), 1.28, 1.33 [2s, each 6H, 2 × C(CH3)3], 1.37, 1.42 [2s, each 3H, C(CH3)3], 2.44, 2.55 [2m, each 2H, (CH2)2], 3.43, 3.47 (2s, each 3H, 2 × OCH3), 3.09 (ddd, 1H, J5′,6′a 8.1 Hz, J6′a,NH 5.5 Hz, J6′a,6′b 13.8 Hz, H-6′a), 3.30–3.41 (m, 2H, H-2′, OH-2′), 3.52 (q, 2H,J 7.1 Hz, CH3CH2N), 3.60–3.78 (m, 4H, OCH2CH2N, H-5′, H-6′b), 3.82 (dd, 1H, J3,4 1.2 Hz, J4,5 4.8 Hz, H-4), 3.90–4.12 (m, 5H, H-3′, H-4′, H-3, H-6a, H-6b), 4.18 (m, 1H, H-5), 4.25 (t, 2H,J 6.1 Hz, CH2OCO), 4.30 (d, 1H, J1′,2′ 8.2 Hz, H-1′), 4.34 (d, 1H, J1,2 6.5 Hz, H-1), 4.40 (dd, 1H, J2,3 7.1 Hz, H-2), 6.67 (bt, 1H, NH), 7.73 (d, 1H, Ar-H-6), 6.86, 7.85 (AA′XX′ system, 4H, Ar-H-8, Ar-H-9, Ar-H-11, Ar-H-12), 8.15 (dd, 1H, J5,6 9.0 Hz, Ar-H-5), 8.37 (d, 1H, J3,5 2.4 Hz, Ar-H-3); 13C NMR (62.9 MHz, CD3CN): δ 12.4 (CH3), 25.1, 26.5, 26.6, 26.9, 27.4, 28.4 [3 × C(CH3)3], 29.1, 30.9 [(CH2)2], 40.9 (C-6′), 46.4 (CH3CH2N), 49.5 (OCH2CH2N), 56.0, 58.0 (2 × OCH3), 66.1 (C-6), 62.3 (CH2OCO), 74.6 (C-2′), 72.7 (C-5′), 75.0 (C-4′), 77.4 (C-2), 77.6 (C-5), 77.8 (C-4), 78.4 (C-3), 80.1 (C-3′), 104.0 (C-1′), 108.2 (C-1), 109.4, 110.6, 110.7 [3 × C(CH3)3], 112.7, 112.7, 118.8, 123.9, 126.8, 127.6, 127.7 (7 × Ar-CH), 134.1 (Ar-C-2), 144.8 (Ar-C-7), 148.2 (Ar-C-4), 153.3, 153.8 (Ar-C-1, Ar-C-10), 172.3, 173.7 (2 × C=O). Anal. Calcd for C43H60ClN5O16 (938.41): C, 55.04; H, 6.44; N, 7.46. Found: C, 55.02; H, 6.42; N, 7.43.


## 2.5. Preparation of Protected GAD13

A solution of6 (1.0 g, 2.78 mmol), azo dye8 (950 mg, 3.52 mmol, 1.25 eq),N,N′-dicyclohexylcarbodiimide (DDC) (688 mg, 3.34 mmol), and 4-dimethylaminopyridine (DMAP) (102 mg, 0.83 mmol) in dry THF (30 mL) was allowed to react in the reaction conditions described for the preparation of11. The flash chromatography over silica gel (1:1 hexane-EtOAc) afforded pure13 (1.47 g, 86% yield) as a yellow foam; Rf 0.31 (4:6 hexane-EtOAc); λmax 407.00 nm (MeOH); 1H NMR (200.13 MHz, CDCl3): δ 1.22 (t, 3H,J 7.0 Hz, CH3), 1.30, 1.33, 1.44, 1.48 [4s, each 3H, 2 × C(CH3)3], 2.47, 2.66 [2m, each 2H, (CH2)2], 3.20 (ddd, 1H, J6a,6b 14.0 Hz, J5,6a 9.0 Hz, J6a,NH 3.8 Hz, H-6a), 3.49 (q, 2H,J 7.0 Hz, CH3CH2N), 3.64 (t, 2H,J 6.2 Hz, OCH2CH2N), 3.71 (ddd, 1H, J5,6b 3.5 Hz, J6b,NH 7.7 Hz, H-6b), 3.87 (ddd, 1H, H-5), 4.18 (dd, 1H, J3,4 7.9 Hz, J4,5 1.8 Hz, H-4), 4.30 (dd, 1H, J1,2 5.0 Hz, J2,3 2.4 Hz, H-2), 4.28 (t, 2H,J 6.2 Hz, CH2OCO), 4.57 (dd, 1H, H-3), 5.50 (d, 1H, H-1), 6.00 (dd, 1H, NH), 6.78 (m, 2H, Ar-H-9, Ar-H-11), 7.37–7.51 (m, 3H, Ar-H-3, Ar-H-4, Ar-H-5), 7.81–7.90 (m, 4H, Ar-H-2, Ar-H-6, Ar-H-8, Ar-H-12); 13C NMR (50.3 MHz, CDCl3): δ 12.2 (CH3), 24.3, 24.9, 25.8, 25.9 [2 × C(CH3)3], 29.3, 30.7 [(CH2)2], 40.0 (C-6), 45.5 (CH3CH2N), 48.7 (OCH2CH2N), 61.5 (CH2OCO), 66.3 (C-5), 70.5 (C-2), 70.7 (C-3), 71.6 (C-4), 96.2 (C-1), 108.7, 109.4 [2 × C(CH3)3], 111.3, 122.1, 125.2, 126.4, 128.9 (Ar-CH), 143.6 (Ar-C-7), 149.9, 153.0, (Ar-C-1, Ar-C-10), 171.4, 172.7 (2 × C=O). Anal. Calcd for C32H42N4O8 (610.70): C, 62.94; H, 6.93; N, 9.17. Found: C, 62.91; H, 6.90; N, 9.15.


## 2.6. Preparation of Protected GAD14

A solution of azo dye10 (152 mg, 0.367 mmol) and amine4 (114 mg, 0.441 mmol, 1.2 eq) in dry THF (4 mL) was stirred at room temperature (10 min). 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM) [13] (122 mg, 0.441 mmol, 1.2 eq) was added and the mixture was stirred at room temperature until azo dye10 was completely disappeared (20 h, TLC, EtOAc). The reaction mixture was concentrated under diminished pressure and the crude residue was partitioned between water (10 mL) and Et2O (10 mL) and the aqueous phase extracted with Et2O (3 × 10 mL). The collected organic phases were dried (Na2SO4), filtered, and concentrated under diminished pressure. Purification of the crude residue (3.10 g) by flash chromatography over silica gel (1:4 hexane-EtOAc) gave pure14 as a red foam solid (230 mg, 95% yield calculated from10); Rf 0.45 (EtOAc); 1H NMR (250.12 MHz, CD3CN): δ 1.19 (t, 3H,J 7.0 Hz, CH3), 1.26, 1.29, 1.37, 1.41 [4s, each 3H, 2 × C(CH3)3], 2.38, 2.52 [2m, each 2H, (CH2)2], 3.12 (ddd, 1H, J6a,6b 13.7 Hz, J5,6a 8.1 Hz, J6a,NH 5.5 Hz, H-6a), 3.36 (ddd, 1H, J5,6b 5.2 Hz, J6b,NH 6.4 Hz, H-6b), 3.54 (q, 2H,J 7.0 Hz, CH3CH2N), 3.68 (t, 2H,J 5.9 Hz, CH2CH2N), 3.84 (ddd, 1H, H-5), 4.17 (dd, 1H, J3,4 7.9 Hz, J4,5 1.9 Hz, H-4), 4.29 (dd, 1H, J1,2 4.9 Hz, J2,3 2.5 Hz, H-2), 4.26 (t, 2H,J 5.9 Hz, CH2OCO), 4.57 (dd, 1H, H-3), 5.41 (d, 1H, H-1), 6.52 (bt, 1H, NH), 6.89, 7.86 (AA′XX′ system, 4H, Ar-H-8, Ar-H-9, Ar-H-11, Ar-H-12), 7.91, 8.31 (AA′XX′ system, 4H, Ar-H-2, Ar-H-3, Ar-H-5, Ar-H-6); 13C NMR (62.9 MHz, CD3CN): δ 12.4 (CH3), 24.5, 25.2, 26.2, 26.3 [2 × C(CH3)3], 30.0, 30.9 [(CH2)2], 40.6 (C-6), 46.3 (CH3CH2N), 49.4 (CH2CH2N), 62.3 (CH2OCO), 66.9 (C-5), 71.4 (C-2), 71.5 (C-3), 72.2 (C-4), 96.1 (C-1), 109.3, 109.8 [2 × C(CH3)3], 112.6, 123.3, 125.7, 126.9 (8 × Ar-CH), 144.3 (Ar-C-7), 148.4 (Ar-C-4), 152.7, 157.7 (Ar-C-1, Ar-C-10), 172.4, 173.6 (2 × C=O). Anal. Calcd for C32H41N5O10 (655.70): C, 58.62; H, 6.30; N, 10.68. Found: C, 58.60; H, 6.28; N, 10.65.


## 2.7. Preparation of Protected GAD15

The condensation of azo dye10 (151 mg, 0.367 mmol) and amine5 (224 mg, 0.441 mmol, 1.2 eq) with 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM) [13] (122 mg, 0441 mmol, 1.2 eq) in dry THF (4 mL) was performed according to the procedure described above for the preparation of14. The flash chromatography over silica gel (1:9 hexane-EtOAc) of the crude reaction product led to pure15 (252 mg, 76% yield calculated from10) as a red foam; Rf 0.32 (EtOAc); 1H NMR (250.12 MHz, CD3CN): δ 1.18 (t, 3H,J 7.0 Hz, CH3), 1.27, 1.33 [2s, each 6H, 2 × C(CH3)3], 1.37, 1.42 [2s, each 3H, C(CH3)3], 2.44, 2.55 [2m, each 2H, (CH2)2], 3.43, 3.46 (2s, each 3H, 2 × OCH3), 3.11 (ddd, 1H, J5′,6′a 8.8 Hz, J6′a,NH 5.2 Hz, J6′a,6′b 13.4 Hz, H-6′a), 3.32 (dd, 1H, J1′,2′ 8.0 Hz, J2′,3′ 7.3 Hz, H-2′), 3.51 (q, 2H,J 7.0 Hz, CH3CH2N), 3.67 (m, 2H,J 6.0 Hz, CH2CH2N), 3.70–3.75 (m, 3H, H-5′, H-6′b, OH-2′), 3.82 (dd, 1H, J4,5 5.2 Hz, J3,4 1.3 Hz, H-4), 3.94 (dd, 1H, J3′,4′ 3.4 Hz, H-3′), 4.00 (dd, 1H, J6a,6b 8.1 Hz, J5,6a 6.3 Hz, H-6a), 4.03 (dd, 1H, J2,3 7.2 Hz, H-3), 4.04–4.10 (m, 2H, H-4′, H-6b), 4.22 (dt, 1H, J5,6a=J5,6b 6.3 Hz, H-5), 4.27 (t, 2H,J 6.0 Hz, CH2OCO), 4.31 (d, 1H, H-1′), 4.35 (d, 1H, J1,2 6.4 Hz, H-1), 4.41 (dd, 1H, H-2), 6.68 (bt, 1H, NH), 6.85, 7.68 (AA′XX′ system, 4H, Ar-H-8, Ar-H-9, Ar-H-11, Ar-H-12), 7.88, 8.29 (AA′XX′ system, 4H, Ar-H-2, Ar-H-3, Ar-H-5, Ar-H-6); 13C NMR (62.9 MHz, CD3CN): δ 12.4 (CH3), 25.1, 26.4, 26.5, 26.9, 27.4, 28.4 [3 × C(CH3)3], 30.9, 29.9 [(CH2)2], 40.9 (C-6′), 46.2 (CH3CH2N), 49.5 (OCH2CH2N), 56.0, 58.0 (2 × OCH3), 62.3 (CH2OCO), 66.0 (C-6), 74.6 (C-2′), 72.6 (C-5′), 75.0 (C-4′), 77.4 (C-2), 77.6 (C-5), 77.7 (C-4), 78.4 (C-3), 80.0 (C-3′), 104.1 (C-1′), 108.2 (C-1), 109.2, 110.4, 110.6 [3 × C(CH3)3], 112.6, 123.3, 125.7, 126.9 (8 × Ar-CH), 144.2 (Ar-C-7), 148.3 (Ar-C-4), 152.7, 157.6 (Ar-C-1, Ar-C-10), 172.3, 173.7 (2 × C=O). Anal. Calcd for C43H61N5O16 (903.37): C, 57.13; H, 6.80; N, 7.75. Found: C, 57.11; H, 6.77; N, 7.72.


## 2.8. Preparation of Deprotected GAD16

A solution of11 (1.07 g, 1.55 mmol) in 90% aqueous CF3COOH (70 mL) was stirred at room temperature until the starting material was completely disappeared (3 h, TLC, EtOAc). The red-violet solution was concentrated under diminished pressure and repeatedly coevaporated with toluene (5 × 30 mL). The crude residue was dissolved in EtOAc (100 mL) and neutralized with saturated aqueous NaHCO3 (20 mL). The aqueous phase was extracted with EtOAc (3 × 50 mL) and the collected layers were dried, filtered, and concentrated under diminished pressure. The residue was triturated with Et2O and the red residue was constituted (NMR) only by the azo dye16 (640 mg, 98% yield) as a mixture of α- and β-pyranose anomers (NMR) in a ratio of 3 : 2, calculated on the basis of the relative C-1 signal intensities. Compound16 was a red solid foam, Rf 0.21 (9:1 EtOAc-MeOH); Selected 1H NMR (200.13 MHz, CD3OD) signals for both anomers: δ 1.06 (t, 3H,J 6.7 Hz, CH3), 2.48–2.30 [m, 4H, (CH2)2], 6.73, 7.64, (AA′XX′ system, 4H, Ar-H-8, Ar-H-9, Ar-H-11, Ar-H-12), 7.54 (bd, 1H, Ar-H-6), 8.00 (dd, 1H, J5,6 9.0 Hz, Ar-H-5), 8.40 (d, 1H, J3,5 2.2 Hz, Ar-H-3); 13C NMR (50.3 MHz, CD3OD): δα-pyranose 40.8 (C-6), 68.8 (C-5), 69.2 (C-4), 69.7 (C-2, C-3), 93.8 (C-1), β-pyranose 40.7 (C-6), 69.1 (C-4), 72.5 (C-3), 73.0, 73.7 (C-2, C-5), 97.8 (C-1), cluster of signals for both anomers: δ 12.5 (CH3), 29.4–30.9 [(CH2)2], 45.9 (CH3CH2N), 48.9 (OCH2CH2N), 62.0 (CH2OCO), 112.2–127.2 (Ar-CH), 133.0 (Ar-C-2), 143.9 (Ar-C-7), 147.0 (Ar-C-4), 152.6, 152.8 (Ar-C-1, Ar-C-10), 172.4, 173.2 (2 × C=O). Calcd for C26H32ClN5O10 (610.01): C, 51.19; H, 5.29; N, 11.48. Found: C, 51.17; H, 5.28; N, 11.45.


## 2.9. Preparation of Deprotected GAD17

Hydrolysis of12 (640 mg, 0.682 mmol) with 90% aqueous CF3COOH (8 mL) was performed as described above for the preparation of16. After 2 h of stirring, the red-violet solution was concentrated under diminished pressure, neutralized, and extracted as described above to give a red foam constituted (NMR) only by the azo dye17 (485 mg, 92% yield) as a mixture of α- and β-pyranose anomers (NMR) in the ratio of 3 : 2, calculated on the basis of the relative C-1 signal intensities. Compound17 was a red solid foam, Rf 0.18 (8:2 EtOAc-MeOH); λmax 493.25 nm (MeOH); Selected 1H NMR (200.13 MHz, CD3OD): δ 1.20 (t, 3H,J 7.1 Hz, CH3, α- and β-pyranose), 2.48, 2.57 [2m, each 2H, (CH2)2, α- and β-pyranose], 3.18 (dd, 1H, J1,2 7.8 Hz, J2,3 9.0 Hz, H-2, β-pyranose), 4.18 (d, 1H, J1′,2′ 8.0 Hz, H-1′, α-pyranose), 4.20 (d, 1H, J1′,2′ 7.8 Hz, H-1′, β-pyranose), 4.25 (bt, 2H,J 6.4 Hz, CH2OCO, α- and β-pyranose), 4.31 (bt, 2H,J 6.6 Hz, CH2OCO, α- and β-pyranose), 4.48 (d, 1H, H-1, β-pyranose), 4.49–4.61 (m, H-2′, α- and β-pyranose), 5.06 (d, 1H, J1,2 3.7 Hz, H-1, α-pyranose), 6.85, 7.83 (AA′XX′ system, 4H, Ar-H-8, Ar-H-9, Ar-H-11, Ar-H-12, α- and β-pyranose), 7.72 (bd, 1H, Ar-H-6, α- and β-pyranose), 8.13 (bd, 1H,J 9.1 Hz, Ar-H-5, α- and β-pyranose), 8.32 (bd, 1H, Ar-H-3, α- and β-pyranose); 13C NMR (62.9 MHz, CD3OD): δα-pyranose 41.3 (C-6′), 62.0 (C-6), 70.5 (C-4′), 71.4 (C-5), 72.1 (C-2′), 72.4 (C-2), 73.0 (C-3), 73.4 (C-3′), 76.0 (C-5′), 81.1 (C-4), 93.7 (C-1′), 105.0 (C-1), β-pyranose 41.2 (C-6′), 62.8 (C-6), 70.4 (C-4′), 72.3 (C-2′), 73.1 (C-3′), 74.5, 74.6 (C-3, C-2), 74.7 (C-5), 76.2 (C-5′), 80.5 (C-4), 98.1 (C-1′), 105.3 (C-1), cluster of signals for both anomers: δ 12.5 (CH3), 30.3, 31.3 [(CH2)2], 46.7 (CH3CH2N), 50.0 (OCH2CH2N), 62.9 (CH2OCO), 112.9–129.9 (7 × Ar-CH), 134.7 (Ar-C-2), 145.6 (Ar-C-7), 148.6 (Ar-C-4), 153.6, 154.3 (Ar-C-1, Ar-C-10), 174.4, 174.9 (2 × C=O). Anal. Calcd for C32H42ClN5O15 (772.15): C, 49.78; H, 5.48; N, 9.07. Found: C, 49.75; H, 5.46; N, 9.05.


## 2.10. Preparation of Deprotected GAD18

The hydrolysis of13 (193 mg, 0.316 mmol) with 90% aqueous CF3COOH (3 mL) was performed as described above for the preparation of16. After 2 h of stirring, the red-violet solution was concentrated under diminished pressure, neutralized (saturated aqueous NaHCO3) until the red-violet colour turned to yellow-orange, and extracted as described above to give a yellow foam constituted (NMR) only by the azo dye18 (160 mg, 95% yield). Compound18 was a mixture of α- and β-pyranose anomers (NMR) in the ratio of 1 : 1, calculated on the basis of the relative C-1 signal intensities; Rf 0.19 (9:1 EtOAc-MeOH); λmax 411.25 nm (MeOH); Selected 1H NMR (200.13 MHz, CD3OD) signals for both anomers: δ 1.21 (t, 3H,J 6.1 Hz, CH3), 2.58 [m, 4H, (CH2)2], 6.83 (m, 2H, Ar-H-9, Ar-H-11), 7.35–7.50 (m, 3H, Ar-H-3, Ar-H-4, Ar-H-5), 7.75–7.83 (m, 4H, Ar-H-2, Ar-H-6, Ar-H-8, Ar-H-12); 13C NMR (62.9 MHz, CD3OD): δα-pyranose 40.1 (C-6), 67.5 (C-5), 69.4, 69.5, 69.7 (C-2, C-3, C-4), 93.6 (C-1), β-pyranose 40.5 (C-6), 70.3 (C-4), 72.1 (C-3), 73.1, 74.1 (C-2, C-5), 98.2 (C-1), cluster of signals for both anomers: δ 11.9 (CH3), 28.5, 28.6 [(CH2)2], 46.2 (CH3CH2N), 52.9 (OCH2CH2N), 59.8 (CH2OCO), 111.1, 122.5, 125.7, 129.5, 129.9 (Ar-CH), 143.2 (Ar-C-7), 150.1, 153.0 (Ar-C-1, Ar-C-10), 174.0, 174.2 (2 × C=O). Anal. Calcd for C26H34N4O8 (530.57): C, 58.86; H, 6.46; N, 10.56. Found: C, 58.83; H, 6.42; N, 10.53.


## 3. Results and Discussion

In the search of new derivatives to be tested as new GADs, we followed the same strategy used for the synthesis of the di-estereal [4, 5] (1) and diethereal [6] (2) compounds. We were interested in preparing structures with different functional groups at the attachment point with the linker in order to evaluate the synthetic accessibility and, at a later stage, the effect of these changes both on the stability during the dyeing process and on the tinctorial properties. To construct the amido-estereal mixed GADs, we used appropriately protected 6-amino-galactose derivatives in either the condensation reaction with monosuccinyl-azo dye or after their conversion into monosuccinyl derivatives. This would allow us to study the two complementary strategies and to identify the best pathway.Treatment of4 [10] and5 [8] with succinic anhydride in methanol (Scheme 1) afforded, in good yields, the corresponding monosuccinyl derivatives6 (76% yield) and7 (98% yield) that were utilized in the GAD synthesisvia route A (Scheme 2). Azo dyes8–10 (Figure 2) were employed as counterpart in the condensation reaction.Scheme 1
Protected aminosugars (4 and5) and their monosuccinylamides (6 and7).Scheme 2
Protected amido-ester GADs prepared through either method A or method B.Figure 2
Monoazo dyes8-9 and monosuccinyl-azo dye10.In particular, the known yellow azo dye (8) [12] and the commercial Disperse Red 13 (9) were used in the preparation of GADs following route A (Scheme 2) while derivative10, prepared by succinylation of commercial alcoholic azo dye Disperse Red 1, [4] was employed in the condensation reaction with the aminosugar derivatives4 [10] and5 [8] (route B).The coupling reactions were performed in THF withN,N′-dicyclohexylcarbodiimide (DCC, method a) or 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM, method b), prepared according to Kunishima et al. [13] from 2-chloro-4,6-dimethoxy-1,3,5-triazine (CDMT) andN-methylmorpholine (NMM) at room temperature. The results obtained in the preparation of protected GADs11–15 are summarised in Table 1. The two possible approaches gave comparable results and the desired products were isolated in good to excellent yields.Table 1
Preparation of amido-ester protected GADs.
Entry
Reactants
Condensation system
Condensation product (yield%)
Sugar moiety
Dye moiety
1
6
9
Method A
11 (74)
2
7
9
Method A
12 (82)
3
6
8
Method A
13 (86)
4
4
10
Method B
14 (95)
5
5
10
Method B
15 (76)
Method A: DCC, DMAP, and THF; method B: DMTMM and THF.It is noteworthy that the succinyl derivatives7 and10 could be used as crudes without affecting the outcome of the condensation reaction. In the case of protected compounds11–13 the final deprotected GADs16–18 (Figure 3) were obtained by simply removing the acetal protecting groups by means of acid hydrolysis as we previously reported [4–6] for type1 and type2 derivatives (90% aqueous CF3COOH, room temperature).Figure 3
Conjugated deprotected GADs16–18.Deprotected GADs were obtained in good yields (92–98%) as red (16-17) or yellow-orange (18) amorphous residues constituted by mixtures of anomers (NMR analysis). Isomeric forms of the16–18 were identified by comparison of their NMR data with those reported for analogous compounds (types1-2) [4–6]. The UV-Vis absorption spectra for a deprotected red GAD (17) and for the deprotected yellow GAD (18) were recorded in MeOH. As for the previous two generations of GADs, [4, 5] the glycoconjugation process did not affect the absorption properties of the dyes and17 and18 were characterised by almost the same λmax values of the parent not conjugated dyes (λmax around 495 nm for the red compounds9,12, and17 and around 410 nm for the yellow-orange compounds8,13, and18).


## 4. Conclusions

In conclusions, we presented the access to the third generation of GADs exploring two complementary routes. The obtained compounds are characterized by different attachment points between the chromophore or the sugar and the linker. The presence of the amido bond could permit a novel synthetic pathway to GADs, for example, by using enzymes [14] for the condensation reaction. Detailed analysis of the stability and tinctorial properties of the third generation of GADs are currently under investigation, the results of which will be published in due course.



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*Source: 235763-2015-07-29.xml*