# New Spectrophotometric and Conductometric Methods for Macrolide Antibiotics Determination in Pure and Pharmaceutical Dosage Forms Using Rose Bengal

Authors: Rania A. Sayed; Wafaa S. Hassan; Magda Y. El-Mammli; Abadalla Shalaby
Journal: Journal of Spectroscopy
 (2013)

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## Abstract

Two Simple, accurate, precise, and rapid spectrophotometric and conductometric methods were developed for the estimation of erythromycin thiocyanate (I), clarithromycin (II), and azithromycin dihydrate (III) in both pure and pharmaceutical dosage forms. The spectrophotometric procedure depends on the reaction of rose bengal and copper with the cited drugs to form stable ternary complexes which are extractable with methylene chloride, and the absorbances were measured at 558, 557, and 560 nm for (I), (II), and (III), respectively. The conductometric method depends on the formation of an ion-pair complex between the studied drug and rose bengal. For the spectrophotometric method, Beer's law was obeyed. The correlation coefficient (r2) for the studied drugs was found to be 0.9999. The molar absorptivity (ε), Sandell’s sensitivity, limit of detection (LOD), and limit of quantification (LOQ) were also calculated. The proposed methods were successfully applied for the determination of certain pharmaceutical dosage forms containing the studied drugs


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## Body

## 1. Introduction

The macrolides are a large group of antibiotics mainly derived from Streptomyces. They have a common macrocyclic lactone ring to which one or more sugars are attached and are all weak bases that are only slightly soluble in water [1]. They are bacteriostatic agents. They inhibit protein synthesis by binding reversibly to 50 S ribosomal subunit of sensitive microorganisms [2]. Macrolides and related drugs have a postantibiotic effect, that is, antibacterial activity persists after concentrations have dropped below the minimum inhibitory concentration [1].Erythromycin is produced by the actinomycete species,Streptomyces erythreus. Erythromycin is a polyhydroxylactone that contains two sugars. The aglycone portion of the molecule, erythranolide, is a 14-membered lactone ring. An amino sugar, desosamine, is attached through a β-glycosidic linkage to the C-5 position of the lactone ring. The tertiary amine of desosamine confers a basic character to erythromycin (pKa 8.8). Through this group, a number of acid salts of the antibiotic have been prepared. A second sugar, cladinose, which is unique to erythromycin, is attached via a β-glycosidic linkage to the C-3 position of the lactone ring [3]. It is commonly used to treat infections caused by gram-positive organisms, Mycoplasma species, and certain susceptible gram-negative and anaerobic bacteria within respiratory tract, skin, soft tissues, and genital tract [4].More recently developed macrolides, including azithromycin and clarithromycin, seem to have essentially similar properties to erythromycin, which is a natural macrolide, though they may differ in their pharmacokinetics. Clarithromycin and, to a lesser extent, azithromycin are more active than erythromycin against opportunistic mycobacteria such asMycobacterium avium complex. Clarithromycin is also used in the treatment of leprosy and in regimens for the eradication of Helicobacter pylori in peptic ulcer disease. Both azithromycin and clarithromycin have activity against protozoa including Toxoplasma gondii. Gastrointestinal disturbances are the most frequent adverse effect but are usually mild and less frequent with clarithromycin and azithromycin than with erythromycin [1].The literature search revealed different techniques for the analysis of the studied macrolides. The British Pharmacopoeia (BP) stated the liquid chromatography method for the assay of erythromycin, clarithromycin and azithromycin [5]. Also, the cited drugs have been analyzed spectrophotometrically in pharmaceutical dosage forms and biological fluids [6–10] and spectrofluorimetrically [11, 12]. Other methods have been published for the cited drugs either in pharmaceutical preparations and biological fluids such as capillary electrophoresis [13, 14], HPLC [15–17], and voltametry [18]. A microbiological method was reported for the assay of erythromycin thiocyanate [19] and azithromycin [20].The majority of the reported methods are HPLC methods which require complex and expensive equipment, intensive sample preparation, and personnel skilled in chromatographic techniques. Also,  Due to weak UV absorbance of macrolide antibiotics, it is difficult to develop a simple method for analysis of the drugs in pharmaceutical applications and biological fluids using conventional UV detection, so there is a need for simple spectrophotometric method for the analysis of the cited drugs in pharmaceutical formulations. So, the aim of this work is to develop simple, sensitive, accurate, and cheap spectrophotometric (through ternary complex formation with rose bengal and copper) and conductometric mehods (through ion pair formation with rose bengal) for the determination of three macrolide drugs in pure form and in the pharmaceutical preparations.Rose bengal has been used as ion-pair-forming agent for determination of chlorphenoxamine hydrcholoride, anhydrous caffeine [21], and oxybuprocaine hydrochloride [22]. It was also used for conductometric determination of clindamycin hydrochloride through ion-pair formation [23], and it was also used for determination of isoniazide, nalidixic acid, and flumequine through formation of ternary complex with Cd(II) [24] and used for determination of barium through formation of ternary complex with 18-crown-6 [25].  Copper was used as a complexing agent for determination of several drugs for example, lincomycin [26], ramipril, perindopril [27] and enalapril maleate [28], lansoprazole and pantoprazole sodium sesquihydrate [29], and risedronate [30].


## 2. Experimental

### 2.1. Instruments

The absorption spectra for all measurements were carried out using shimadzu recording spectrophotometer UV 1201 equipped with 10 mm matched quartz cells. Conductometer model 470 portable conductivity/ TDS meter, 25 DEG.C-C10 dip-type cell was used with a cell constant, K cell, of 1.09. Digital analyzer pH meter (USA) was used.


### 2.2. Reagents and Materials

All chemicals and materials were of analytical grade, and bidistilled water was used throughout the work.(i)
Erythromycin thiocyanate, Erythrocin film tablets labelled to contain erythromycin  stearate equivalent to 500 mg erythromycin per tablet (Kahira Pharm. & Chem.Ind.Co.), and erythrocin pharco powder for oral suspension labelled to contain erythromycin  ethylsuccinate equivalent to 200 mg erythromycin to each 5 mL reconstituted suspension (Pharco Company For Phrmaceuticals, Egypt).(ii)
Clarithromycin, Klarimix tablets labelled to contain 250 mg clarithromycin per tablet, and Klarimix powder for oral suspension labelled to contain 125 mg clarithromycin to each 5 mL reconstituted suspension (SIGMA Pharmaceutical Industries, Egypt).(iii)
Azithromycin dihydrate, Zithrokan capsules, Azrolid tablets (Amriya For Phrmaceutical Industries, Egypt) labelled to contain 524.1 mg azithromycin dihydrate per capsule/tablet, and Zithrokan powder for oral suspension labelled to contain 200 mg azithromycin to each 5 mL reconstituted suspension (Hikma Pharma, 6th of October city, Egypt).(iv)
Rose bengal (Aldrich, Germany) was used as 3 mg/mL in double-distilled water for the spectrophotometric method. For conductometric method, 3 × 10−3 M in double distilled water for (I), in  50% (v/v) ethanol for (II), and in 50% (v/v) methanol for (III).(v)
Copper(II) chloride (El-Nasr chemical pharmaceutical company, Egypt)  solution was used as 3 mg/mL in double-distilled water.(vi)
Glucose, Sucrose, sodium chloride, carboxy methyl cellulose sodium, calcium carbonate, magnesium stearate (El-Nasr chemical pharmaceutical company, Egypt).All drugs whose purity was found to be 100.12% according to the reported method [6] were used as received.


### 2.3. Preparation of Sample Solutions

(i)
For the spectrophotometric method, solution of 0.5 mg/mL for (I) and (II) and of 0.2 mg/mL for (III) was dissolved in low amount of ethanol and completed with double distilled water to the mark.
(ii)
For conductometric method, solution of 1 mg/mL was dissolved in low amount of methanol for (I) and completed with double distilled water to the mark, in 50% (v/v) ethanol for (II) and 50% (v/v) methanol for (III).


### 2.4. Recommended Procedures and Calibration Curves

#### 2.4.1. Spectrophotometric Measurements

Into a series of 60 mL separating funnels, aliquots of drug solutions equivalent to 1.5–6, 0.75–4, and 0.4–2 mg of erythromycin thiocyanate (I), clarithromycin (II), azithromycin dihydrate (III), respectively, were transferred, then specific volumes of Cu (II) chloride solution (3 mg/mL) were added followed by specified amounts of rose bengal (3 mg/mL) and left for 5 minutes in case of Clarithromycin (II) (Table1). The complex was extracted with 3 × 3 mL portions of methylene chloride. The solutions were vigorously shaken for one minute and the organic layer filtered over anhydrous sodium sulphate into 10 mL volumetric flasks, then the volumes were completed to 10 mL with methylene chloride. The absorbances of the colored solutions were measured at 558, 557, and 560 nm for (I), (II), and (III), respectively, against reagent blank treated similarly (Figure 1). All measurements were made at ambient temperature.Table 1
Quantitative parameters for the spectrophotometric determination of erythromycin thiocyanate (I), clarithromycin (II), and azithromycin dihydrate (III) with rose bengal copper.
Parameter
(I)
(II)
(III)
λ max (nm)
558
557
560
Beer's law ranges (μg/mL)
15–60
7.5–40
4–20
Regression equation**
Slope (b)
0.0193
0.0209
0.0617
Intercept (a)
−0.1413
−0.022
−0.1247
Correlation coefficient (r2)
0.9999
0.9999
0.9999
LODμg/mL
1
0.93
0.31
LOQμg/mL
3.04
2.8
0.95
Ringbom optimum concentrationrange (μg/mL)
20–50
10–35
9–16
Sandell's sensitivityμg·cm−2
0.07
0.05
0.02
ε (×104) L·mol−1·cm−1
1.12
1.47
3.78
*Average of three experiments.
**A =a + bc.Figure 1
Absorption spectra for (a) the reaction of 13μg/mL azithromycin dihydrate with 0.5 mL Cu(II) chloride 0.3% w/v and 0.2 mL 0.3% w/v rose bengal, (b) 13 μg/mL azithromycin dihydrate with 0.2 mL rose bengal 0.3% w/v, (c) 13 μg/mL azithromycin dihydrate with 0.5 mL Cu(II) chloride 0.3% w/v, (d) 0.2 mL rose bengal 0.3% w/v and 0.5 mL Cu(II) chloride 0.3% w/v, (e) 0.2 mL rose bengal 0.3% w/v, and (f) 0.5 mL Cu(II) chloride 0.3% w/v.


#### 2.4.2. Conductometric Titrations

Aliquot of sample solution containing 1–15 mg of the cited  drugs was transferred to a 50 mL calibrated flask, and volume was made up to the mark using bidistilled water for (I), 50% (v/v) ethanol for (II), and 50% (v/v) methanol for (III). The contents of the calibrated flask were transferred to a beaker, and the conductivity cell was immersed. 3 × 10−3 M rose bengal was used for titration. The conductance was measured subsequently to each addition of reagent solution and, after thorough stirring for two minutes, corrected for dilution effect [31] by means of the following equation, assuming that conductivity is a linear function of dilution
(1)Ωcorrect-1=Ωobs-1[(v1+v2)v1],
where Ωobs-1 is the observed electrolytic conductivity, v1 is the initial volume, and v2 is the volume of reagent added. A graph of corrected conductivity versus the volume of added titrant was constructed and the endpoint was determined (Figure 7).


### 2.5. Procedure for Pharmaceutical Preparations

#### 2.5.1. For Spectrophotometric Method

The contents of five capsules were removed and weighed accurately. The combined contents were mixed, and a quantity of the powder equivalent to 25 mg for (I) and (II)  and  equivalent to 10 mg for (III) was extracted with low amount of ethanol in 50 mL volumetric flask. The volumes were completed to the mark with double-distilled water filtered, and the assay was completed as described under general procedure. The same procedures were followed with tablets taking an accurately weighed amount of ten combined powdered tablets. The same procedures were followed with oral suspension taking an accurately measured volume of the freshly reconstituted oral suspension equivalent to the specified concentration.


#### 2.5.2. For Conductometric Method

The same procedure was followed as in the spectrophotometric method but the quantity of the powder taken from tablets or capsules or the accurately measured volume of the freshly reconstituted oral suspension is equivalent to 100 mg for all drugs and was extracted by least amount of methanol for (I), 50% (v/v) ethanol for (II), and 50% (v/v) methanol for (III), filtered, and transferred to a 100 mL volumetric flask, completed to the mark using distilled water for (I), 50% (v/v) ethanol for (II), and in 50% (v/v) methanol for (III) and the assay was completed as described under general procedure.


## 2.1. Instruments

The absorption spectra for all measurements were carried out using shimadzu recording spectrophotometer UV 1201 equipped with 10 mm matched quartz cells. Conductometer model 470 portable conductivity/ TDS meter, 25 DEG.C-C10 dip-type cell was used with a cell constant, K cell, of 1.09. Digital analyzer pH meter (USA) was used.


## 2.2. Reagents and Materials

All chemicals and materials were of analytical grade, and bidistilled water was used throughout the work.(i)
Erythromycin thiocyanate, Erythrocin film tablets labelled to contain erythromycin  stearate equivalent to 500 mg erythromycin per tablet (Kahira Pharm. & Chem.Ind.Co.), and erythrocin pharco powder for oral suspension labelled to contain erythromycin  ethylsuccinate equivalent to 200 mg erythromycin to each 5 mL reconstituted suspension (Pharco Company For Phrmaceuticals, Egypt).(ii)
Clarithromycin, Klarimix tablets labelled to contain 250 mg clarithromycin per tablet, and Klarimix powder for oral suspension labelled to contain 125 mg clarithromycin to each 5 mL reconstituted suspension (SIGMA Pharmaceutical Industries, Egypt).(iii)
Azithromycin dihydrate, Zithrokan capsules, Azrolid tablets (Amriya For Phrmaceutical Industries, Egypt) labelled to contain 524.1 mg azithromycin dihydrate per capsule/tablet, and Zithrokan powder for oral suspension labelled to contain 200 mg azithromycin to each 5 mL reconstituted suspension (Hikma Pharma, 6th of October city, Egypt).(iv)
Rose bengal (Aldrich, Germany) was used as 3 mg/mL in double-distilled water for the spectrophotometric method. For conductometric method, 3 × 10−3 M in double distilled water for (I), in  50% (v/v) ethanol for (II), and in 50% (v/v) methanol for (III).(v)
Copper(II) chloride (El-Nasr chemical pharmaceutical company, Egypt)  solution was used as 3 mg/mL in double-distilled water.(vi)
Glucose, Sucrose, sodium chloride, carboxy methyl cellulose sodium, calcium carbonate, magnesium stearate (El-Nasr chemical pharmaceutical company, Egypt).All drugs whose purity was found to be 100.12% according to the reported method [6] were used as received.


## 2.3. Preparation of Sample Solutions

(i)
For the spectrophotometric method, solution of 0.5 mg/mL for (I) and (II) and of 0.2 mg/mL for (III) was dissolved in low amount of ethanol and completed with double distilled water to the mark.
(ii)
For conductometric method, solution of 1 mg/mL was dissolved in low amount of methanol for (I) and completed with double distilled water to the mark, in 50% (v/v) ethanol for (II) and 50% (v/v) methanol for (III).


## 2.4. Recommended Procedures and Calibration Curves

### 2.4.1. Spectrophotometric Measurements

Into a series of 60 mL separating funnels, aliquots of drug solutions equivalent to 1.5–6, 0.75–4, and 0.4–2 mg of erythromycin thiocyanate (I), clarithromycin (II), azithromycin dihydrate (III), respectively, were transferred, then specific volumes of Cu (II) chloride solution (3 mg/mL) were added followed by specified amounts of rose bengal (3 mg/mL) and left for 5 minutes in case of Clarithromycin (II) (Table1). The complex was extracted with 3 × 3 mL portions of methylene chloride. The solutions were vigorously shaken for one minute and the organic layer filtered over anhydrous sodium sulphate into 10 mL volumetric flasks, then the volumes were completed to 10 mL with methylene chloride. The absorbances of the colored solutions were measured at 558, 557, and 560 nm for (I), (II), and (III), respectively, against reagent blank treated similarly (Figure 1). All measurements were made at ambient temperature.Table 1
Quantitative parameters for the spectrophotometric determination of erythromycin thiocyanate (I), clarithromycin (II), and azithromycin dihydrate (III) with rose bengal copper.
Parameter
(I)
(II)
(III)
λ max (nm)
558
557
560
Beer's law ranges (μg/mL)
15–60
7.5–40
4–20
Regression equation**
Slope (b)
0.0193
0.0209
0.0617
Intercept (a)
−0.1413
−0.022
−0.1247
Correlation coefficient (r2)
0.9999
0.9999
0.9999
LODμg/mL
1
0.93
0.31
LOQμg/mL
3.04
2.8
0.95
Ringbom optimum concentrationrange (μg/mL)
20–50
10–35
9–16
Sandell's sensitivityμg·cm−2
0.07
0.05
0.02
ε (×104) L·mol−1·cm−1
1.12
1.47
3.78
*Average of three experiments.
**A =a + bc.Figure 1
Absorption spectra for (a) the reaction of 13μg/mL azithromycin dihydrate with 0.5 mL Cu(II) chloride 0.3% w/v and 0.2 mL 0.3% w/v rose bengal, (b) 13 μg/mL azithromycin dihydrate with 0.2 mL rose bengal 0.3% w/v, (c) 13 μg/mL azithromycin dihydrate with 0.5 mL Cu(II) chloride 0.3% w/v, (d) 0.2 mL rose bengal 0.3% w/v and 0.5 mL Cu(II) chloride 0.3% w/v, (e) 0.2 mL rose bengal 0.3% w/v, and (f) 0.5 mL Cu(II) chloride 0.3% w/v.


### 2.4.2. Conductometric Titrations

Aliquot of sample solution containing 1–15 mg of the cited  drugs was transferred to a 50 mL calibrated flask, and volume was made up to the mark using bidistilled water for (I), 50% (v/v) ethanol for (II), and 50% (v/v) methanol for (III). The contents of the calibrated flask were transferred to a beaker, and the conductivity cell was immersed. 3 × 10−3 M rose bengal was used for titration. The conductance was measured subsequently to each addition of reagent solution and, after thorough stirring for two minutes, corrected for dilution effect [31] by means of the following equation, assuming that conductivity is a linear function of dilution
(1)Ωcorrect-1=Ωobs-1[(v1+v2)v1],
where Ωobs-1 is the observed electrolytic conductivity, v1 is the initial volume, and v2 is the volume of reagent added. A graph of corrected conductivity versus the volume of added titrant was constructed and the endpoint was determined (Figure 7).


## 2.4.1. Spectrophotometric Measurements

Into a series of 60 mL separating funnels, aliquots of drug solutions equivalent to 1.5–6, 0.75–4, and 0.4–2 mg of erythromycin thiocyanate (I), clarithromycin (II), azithromycin dihydrate (III), respectively, were transferred, then specific volumes of Cu (II) chloride solution (3 mg/mL) were added followed by specified amounts of rose bengal (3 mg/mL) and left for 5 minutes in case of Clarithromycin (II) (Table1). The complex was extracted with 3 × 3 mL portions of methylene chloride. The solutions were vigorously shaken for one minute and the organic layer filtered over anhydrous sodium sulphate into 10 mL volumetric flasks, then the volumes were completed to 10 mL with methylene chloride. The absorbances of the colored solutions were measured at 558, 557, and 560 nm for (I), (II), and (III), respectively, against reagent blank treated similarly (Figure 1). All measurements were made at ambient temperature.Table 1
Quantitative parameters for the spectrophotometric determination of erythromycin thiocyanate (I), clarithromycin (II), and azithromycin dihydrate (III) with rose bengal copper.
Parameter
(I)
(II)
(III)
λ max (nm)
558
557
560
Beer's law ranges (μg/mL)
15–60
7.5–40
4–20
Regression equation**
Slope (b)
0.0193
0.0209
0.0617
Intercept (a)
−0.1413
−0.022
−0.1247
Correlation coefficient (r2)
0.9999
0.9999
0.9999
LODμg/mL
1
0.93
0.31
LOQμg/mL
3.04
2.8
0.95
Ringbom optimum concentrationrange (μg/mL)
20–50
10–35
9–16
Sandell's sensitivityμg·cm−2
0.07
0.05
0.02
ε (×104) L·mol−1·cm−1
1.12
1.47
3.78
*Average of three experiments.
**A =a + bc.Figure 1
Absorption spectra for (a) the reaction of 13μg/mL azithromycin dihydrate with 0.5 mL Cu(II) chloride 0.3% w/v and 0.2 mL 0.3% w/v rose bengal, (b) 13 μg/mL azithromycin dihydrate with 0.2 mL rose bengal 0.3% w/v, (c) 13 μg/mL azithromycin dihydrate with 0.5 mL Cu(II) chloride 0.3% w/v, (d) 0.2 mL rose bengal 0.3% w/v and 0.5 mL Cu(II) chloride 0.3% w/v, (e) 0.2 mL rose bengal 0.3% w/v, and (f) 0.5 mL Cu(II) chloride 0.3% w/v.


## 2.4.2. Conductometric Titrations

Aliquot of sample solution containing 1–15 mg of the cited  drugs was transferred to a 50 mL calibrated flask, and volume was made up to the mark using bidistilled water for (I), 50% (v/v) ethanol for (II), and 50% (v/v) methanol for (III). The contents of the calibrated flask were transferred to a beaker, and the conductivity cell was immersed. 3 × 10−3 M rose bengal was used for titration. The conductance was measured subsequently to each addition of reagent solution and, after thorough stirring for two minutes, corrected for dilution effect [31] by means of the following equation, assuming that conductivity is a linear function of dilution
(1)Ωcorrect-1=Ωobs-1[(v1+v2)v1],
where Ωobs-1 is the observed electrolytic conductivity, v1 is the initial volume, and v2 is the volume of reagent added. A graph of corrected conductivity versus the volume of added titrant was constructed and the endpoint was determined (Figure 7).


## 2.5. Procedure for Pharmaceutical Preparations

### 2.5.1. For Spectrophotometric Method

The contents of five capsules were removed and weighed accurately. The combined contents were mixed, and a quantity of the powder equivalent to 25 mg for (I) and (II)  and  equivalent to 10 mg for (III) was extracted with low amount of ethanol in 50 mL volumetric flask. The volumes were completed to the mark with double-distilled water filtered, and the assay was completed as described under general procedure. The same procedures were followed with tablets taking an accurately weighed amount of ten combined powdered tablets. The same procedures were followed with oral suspension taking an accurately measured volume of the freshly reconstituted oral suspension equivalent to the specified concentration.


### 2.5.2. For Conductometric Method

The same procedure was followed as in the spectrophotometric method but the quantity of the powder taken from tablets or capsules or the accurately measured volume of the freshly reconstituted oral suspension is equivalent to 100 mg for all drugs and was extracted by least amount of methanol for (I), 50% (v/v) ethanol for (II), and 50% (v/v) methanol for (III), filtered, and transferred to a 100 mL volumetric flask, completed to the mark using distilled water for (I), 50% (v/v) ethanol for (II), and in 50% (v/v) methanol for (III) and the assay was completed as described under general procedure.


## 2.5.1. For Spectrophotometric Method

The contents of five capsules were removed and weighed accurately. The combined contents were mixed, and a quantity of the powder equivalent to 25 mg for (I) and (II)  and  equivalent to 10 mg for (III) was extracted with low amount of ethanol in 50 mL volumetric flask. The volumes were completed to the mark with double-distilled water filtered, and the assay was completed as described under general procedure. The same procedures were followed with tablets taking an accurately weighed amount of ten combined powdered tablets. The same procedures were followed with oral suspension taking an accurately measured volume of the freshly reconstituted oral suspension equivalent to the specified concentration.


## 2.5.2. For Conductometric Method

The same procedure was followed as in the spectrophotometric method but the quantity of the powder taken from tablets or capsules or the accurately measured volume of the freshly reconstituted oral suspension is equivalent to 100 mg for all drugs and was extracted by least amount of methanol for (I), 50% (v/v) ethanol for (II), and 50% (v/v) methanol for (III), filtered, and transferred to a 100 mL volumetric flask, completed to the mark using distilled water for (I), 50% (v/v) ethanol for (II), and in 50% (v/v) methanol for (III) and the assay was completed as described under general procedure.


## 3. Results and Discussion

Optimization of Variables. Ternary complexes have been widely used in spectrophotometric analysis. The absorption spectrum in aqueous medium of rose bengal shows an absorption peak with λmax 540 nm. Upon addition of any of the three cited drugs to a mixture of Cu(II) and rose bengal solution, it was found that a pink soluble complex was formed, and the absorbance maximum of Cu(II) rose bengal originally at 540 nm was abridged to a shoulder with a new absorption maxima at 558, 557, and 560 nm for (I), (II), and (III), respectively. The absorption spectrum shows that the ternary complexes formed between rose bengal and copper with the cited drugs have higher values of molar extinction coefficient than the binary complexes for the spectrophotometric method (Figure 1). Investigations were carried out to establish the most favorable conditions for the ternary complex formation for the spectrophotometric method of the cited drugs with rose bengal and copper to achieve maximum color development, and for ion-pair formation for the conductometric method to achieve the endpoint.


### 3.1. Conditions for Spectrophotometric Method

#### 3.1.1. Effect of pH

In a trial to elucidate the optimum medium for the quantitative determination of the studied drugs, the effect of different acetate and phosphate buffers of pH of range 2–10 was examined. It was found that buffer had no effect on the reaction.


#### 3.1.2. Effect of Cu(II) Chloride Volume

The effect of Cu(II) chloride volume was also tested by using varying amounts (0.1–2 mL)  of Cu(II) chloride (3 mg/mL). The results showed that 0.2 mL, 1.5 mL, and 0.5 mL of Cu(II) chloride (3 mg/mL) for (I), (II), and (III), respectively, were sufficient for the production of maximum and reproducible colour intensity (Figure2).Figure 2
Effect of volume of 0.3% w/v Cu(II) chloride on the ternary complex formed with 40μg/mL erythromycin thiocyanate and 1.5 mL rose bengal 0.3% w/v; 25 μg/mL clarithromycin and 0.5 mL rose bengal 0.3% w/v; 13 μg/mL azithromycin dihydrate and 0.2 mL rose bengal 0.3% w/v.


#### 3.1.3. Effect of Rose Bengal Volume

Highest constant absorbance was obtained on using 1.5 mL, 0.5 mL, and 0.2 mL of rose bengal (3 mg/mL) for (I), (II), and (III), respectively, (Figure3).Figure 3
Effect of volume of 0.3% w/v rose bengal on the ternary complex formed with 40μg/mL erythromycin thiocyanate and 0.2 mL Cu(II) chloride 0.3% w/v; 25 μg/mL clarithromycin and 1.5 mL Cu(II) chloride 0.3% w/v; 13 μg/mL azithromycin dihydrate and 0.5 mL Cu(II) chloride 0.3% w/v.


#### 3.1.4. Effect of Order of Addition

The most suitable sequence was drug, Cu(II) chloride, and then rose bengal for all drugs for  the production of the highest colour intensity, while the other sequences produced lower absorbance values.


#### 3.1.5. Effect of Reaction Time and Stability

Maximum colour intensity was attained immediately for the studied drugs except for (II); it needs 5 minutes to give complete complexation, and increasing time beyond this had no effect on absorption. The obtained colour was stable for at least 1 hour for all drugs at room temperature (25°C ± 2). The formation constants have been calculated by using the molar ratio method [32]. The formation constants (Kf) of the reaction product were found to be 7.13 × 104, 7.6 × 105, and 1.5 × 106 L·mol−1 for (I), (II), and (III), respectively. This high value indicates a very stable reaction product. The Gibbs free energy change of the reaction (ΔG) was also calculated and was found to be −2.78 × 104, −3.35 × 104, and −3.52 × 104 K·J·mole−1 for (I), (II), and (III), respectively. The negative value of ΔG points out to the spontaneous nature of the reaction [33].


#### 3.1.6. Effect of the Extraction Time and Number of Extractions

Shaking the reaction mixture with the extracting organic solvent is required for complete extraction of the ternary complex formed. It was found that 1 minute is enough time for complete colour extraction. Complete extraction was attained by triple extraction with 3 × 3 mL of methylene chloride for all drugs.


#### 3.1.7. Effect of Solvent

Many organic solvents were tried to extract the ternary complexes formed (chloroform, methylene chloride, ethylene chloride, benzene, toluene, and ethyl acetate). The results obtained indicated that methylene chloride is the appropriate solvent for extraction for all drugs.


#### 3.1.8. Determination of Stoichiometric Balance Using Molar Ratio Method [34]

The reaction stoichiometry between erythromycin thiocyanate (I), clarithromycin (II), azithromycin dihydrate (III)  with Cu(II) chloride and rose bengal was studied by molar ratio method using an equimolar (2.5 × 10−4 M) solution of erythromycin thiocyanate (I), clarithromycin (II), and (1.25 × 10−4 M) solutions of azithromycin dihydrate (III), Cu(II) chloride, and rose bengal of the same molarities. The obtained results showed that the composition of the ternary complexes was 1 : 1 : 2 for erythromycin thiocyanate (I) and 1 : 2 : 2 for clarithromycin (II) and azithromycin dihydrate (III) : Cu(II) chloride: rose bengal (Figures 4, 5, and 6).Figure 4
Molar ratio method plot for 2.5 × 10−4 M erythromycin thiocyanate and 2.5 × 10−4 M Cu(II) chloride in presence of excess rose bengal (1.5 mL 0.3%); 2.5 × 10−4 M clarithromycin and 2.5 × 10−4 M Cu(II) chloride in presence of excess rose bengal (0.5 mL 0.3%); 1.25 × 10−4 M azithromycin dihydrate and 1.25 × 10−4 M Cu(II) chloride in presence of excess rose bengal (0.2 mL 0.3%) VR = Cu(II) chloride and Vd = Drug.Figure 5
Molar ratio method plot for 2.5 × 10−4 M erythromycin thiocyanate with 2.5 × 10−4 M rose bengal in presence of excess Cu(II) chloride (0.2 mL 0.3%); 2.5 × 10−4 M clarithromycin with 2.5 × 10−4 M rose bengal in presence of excess Cu(II) chloride (1.5 mL 0.3%); 1.25 × 10−4 M azithromycin dihydrate with 1.25 × 10−4 M rose bengal in presence of excess Cu(II) chloride (0.5 mL 0.3%). Vr  = rose bengal and Vd = Drug.Figure 6
Molar ratio method plot for 2.5 × 10−4 M rose bengal with 2.5 × 10−4 M Cu(II) chloride in presence of excess erythromycin thiocyanate (30 μg·mL−1); 2.5 × 10−4 M rose bengal with 2.5 × 10−4 M Cu(II) chloride in presence of excess clarithromycin (30 μg·mL−1); 1.25 × 10−4 M rose bengal with 1.25 × 10−4 M Cu(II) chloride in presence of excess azithromycin dihydrate (10 μg·mL−1). Vr = rose bengal and VR = Cu(II) chloride.Figure 7
Conductometric titration curve of 15 mg clarithromycin versus 3 × 103 M rose bengal.Scheme1 illustrates the mechanism of the reaction of clarithromycin, rose bengal and copper(II) chloride. The lactone ring in clarithromycin is substituted with a number of hydroxyl functional groups. These groups are positioned in suitable configuration for interaction with metal ions. So, copper cation forms a chelate with the lone pair of electrons of the two hydroxyl groups at C11 and C12. Also copper can form a chelate with the lone pair of electrons of the tertiary amine group nitrogen and the oxygen of the β-glycosidic linkage. So, the azithromycin-copper chelate bears an overall positive charge of the original two metal ions, that is, four positive charges. This cationic complex associates with two molecules of rose bengal anions to form the ternary complex.Scheme 1
Proposal Mechanism for the reaction between clarithromycin, rose bengal and copper(II) chloride. clarithromycin-Cu-rose bengal complex.


### 3.2. Conditions for Conductometric Method

Investigations were carried out to establish the most suitable conditions for the ion associates formation of the cited drugs with rose bengal to attain sharp endpoint. The optimum conditions for performing the titration in a quantitative manner were elucidated as described later.


#### 3.2.1. Reaction Medium

Titrations in different media were attempted to obtain the best results. Preliminary experiments were in:(i)
aqueous drug solution for (I) only (prepared by dissolving 100 mg drug in 2 mL methanol then completing to 100 mL with bidistilled water) with aqueous reagent solution,(ii)
ethanol drug solution with ethanol reagent solution,(iii)
drug solution with reagent solution, both in ethanol-water (50%, v/v) mixture,(iv)
methanol drug solution with methanol reagent solution (except for (II) as it is insoluble in methanol),(v)
drug solution with reagent solution, both in methanol-water mixture (50% v/v) (except for (II) as it is insoluble in methanol),(vi)
drug solution with reagent solution, both in acetone-water (50% v/v) mixture.Preliminary experiments showed that procedure in aqueous media was the most suitable for successful results for (I), 50% (v/v) ethanol for (II), and 50% (v/v) methanol for (III).


#### 3.2.2. Reagent’s Concentration

Different concentrations of rose bengal solution were tried ranging from 2 × 10−2 to 5 × 10−4 molar solutions. The optimum concentration of the reagent was 3 × 10−3 M in titration of the studied drug to achieve a constant and highly stable conductance reading within 1-2 min of mixing. Concentrations less than these limits led to unstable readings, and more time was needed to obtain constant conductance values.Representative titration curve is shown in (Figure7). Two straight lines are obtained, intersecting at the endpoint; the first branch ascending, and the second has conductance values that would slightly increase after the equivalence point. The increase of conductance may be attributed to the formation of ion pair in solution as a result of the complexation reaction. After the end-point, the titration curves indicate a slightly increased value of conductance, despite the excess of the reagent. This may be due to further ionic condensation, leading to species of lower mobility.The conductometric titrations of different volumes of 3 × 10−3 M rose bengal solution were performed. The results show an obvious maximum in the conductance curve at drug-reagent molar ratio of 2 : 1 for (I) and (II) and 1 : 1 for (III). The reactions may be represented by (for (I))
(2)[RB]-2+2[ErythN(Me)2]→H+[RB]-2[ErythNH(Me)2]2+2
where [RB]−2 rose bengal anion [ErythN(Me)2] erythromycin thiocyanate [ErythNH(Me)2]+2 protonated erythromycin thiocyanate.Erythromycin thiocyanate tertiary amine nitrogen is protonated, so attraction occurs between it and rose bengal anion forming ion-pair complex.The conductance measured before the addition of the titrant (volume of rose bengal equals zero) is mainly due to the hydrogen ions mobility. These hydrogen ions result from two sources:(1)
the active methylene group in the drug structure as in Scheme2 which has the ability to make self protonation to the drug(2)
the protons coming from the alcohols used in solubility, as these alcohols have certain acidity character so can protonate the amino group in drug structure.Scheme 2
*The active methylene group in macrolide structure.So, there is no need for acidic buffer use for drug protonation. It was expected that the conductance values would remain constant or slightly increase after the equivalence point. However, the conductance of the solution decreased. This may be due to interaction of the protons available in the titration medium with the added reagent. The results from the conductometic titrations are summarized in Table4. The data show that accurate results were obtained with good recoveries and low standard deviation values. The optimum concentration ranges for determination of the cited drugs were in the range of 1–15 mg mL−1 for all drugs. At such ranges, sharp inflections (Figure 7) and stable conductance reading were obtained.


### 3.3. Method Validation

#### 3.3.1. For the Spectrophotometric Method

Linearity. Under the optimum conditions described, standard calibration curves for erythromycin thiocyanate (I), clarithromycin (II), and azithromycin dihydrate (III) with rose bengal and copper were constructed by plotting absorbance against concentration. Conformity with Beer’s law was evident in the concentration range of the final dilution cited in Table 1. Beer’s law holds over the concentration ranges of 15–60, 7.5–40, and 4–20 μg·mL−1 for (I), (II), and (III), respectively. The linear regression equation for each drug was listed in Table 1. The correlation coefficient was 0.9999 indicating good linearity over the working concentration range.Sensitivity. The detection limit (LOD) for the proposed method was calculated using the following equation according to the ICH [35]:
(3)LOD=3.3σS,
where σ = the standard deviation of replicate blank responses (under the same conditions as for sample analysis), and  S = the slope of the calibration curve.The limits of quantification, LOQ, is defined as(4)LOQ=10σS.
According to the previous equations, the LODs and LOQs were calculated as in Table 1. Their values confirm the sensitivity of the proposed method.Accuracy and Precision. In order to determine the accuracy and precision of the proposed method, solutions containing one concentration of each drug were prepared and analysed in seven replicates. The relative standard deviation (RSD%) as precision and percentage relative error (Er%) as accuracy of the suggested method were calculated at 95% confidence levels and can be considered satisfactory. The percentage relative error was calculated according the following equation:
(5)Er%=[found-addedadded]×100.The inter- and intraday precisions and accuracy results are shown in Table5. The analytical results for accuracy and precision show that the proposed method has good repeatability and reproducibility.Interference. The effect of presence of common excipients such as glucose, sucrose, sodium chloride, carboxy methyl cellulose sodium, calcium carbonate, and magnesium stearate was studied. Table 6 presents the results of determination of clarithromycin as a representative example using the proposed procedure in the presence of aforementioned ingredients. The tolerance ratio was defined as the concentration which gave an error of ±3.0% in the determination of drugs. Results indicate that the tolerance ratio of drug excipient, (M : M), is 1 : 50 for glucose and 1 : 100 for sucrose. While there were no interference from the presence of sodium chloride, carboxy methyl cellulose sodium, calcium carbonate, and magnesium stearate. Interference due to glucose and sucrose was further investigated by studying their reaction with Cu(II) chloride and rose bengal. The study revealed that no interference could be observed at 557 nm, and an interaction product was obtained at λmax 498 in tolerance ratios 1 : 1 and 1 : 10 for glucose and 1 : 1, 1 : 10, 1 : 50 for sucrose. However, interference starts to appear at λmax 557 upon using 50 times, 100-fold excess of the molar concentration of clarithromycin for glucose and sucrose, respectively, as shown in Figure 8.Figure 8
Absorption spectra of (a) 2.5 × 10−2 M glucose with 1.5 mL Cu(II) chloride 0.3% w/v and 0.5 mL 0.3% w/v rose bengal, (b) 5 × 10−2 M sucrose with 1.5 mL Cu(II) chloride 0.3% w/v and 0.5 mL 0.3% w/v rose bengal.


#### 3.3.2. For the Conductometric Method

In order to address the validity of the proposed method, statistical analysis of the data obtained from its application on the cited drugs in the pure form and in formulations was performed. The results revealed in Tables5 and 6 showed that the proposed method is satisfactorily accurate, precise, and reproducible over a concentration range of 1–15 mg. Results for the determination of the studied drugs using the previously mentioned method were compared with results from reported one [6], Table 7.


### 3.4. Analytical Applications

The proposed methods were applied to determine the studied drugs in their pharmaceutical dosage forms. Satisfactory results were obtained. The recovery of each drug was calculated by comparing the concentration obtained from the spiked mixtures with those of the drug. The results of analysis of the commercial dosage forms and the recovery study are shown in Tables2, 3, and 4. The results obtained were compared with the reported method [6]. No significant differences were found between the proposed methods and reported method. Statistical comparison of the results was performed using Student’s t-test and variance ratio F-test at 95% confidence level (Table 7).Table 2
Determination of erythromycin thiocyanate and clarithromycin in their pharmaceutical dosage forms through complexation with Cu(II) chloride and rose bengal by spectrophotometric procedure*.
Erythrocin tablets
Erythrocin Pharco suspension
Klarimix tablets
Klarimix suspension
Claimed to be takenμg/mL
Authentic addedμg/mL
Found conc.μg/mL
Recovery %
Claimed to be takenμg/mL
Authentic addedμg/mL
Found conc.μg/mL
Recovery %
Claimed to be takenμg/mL
Authentic addedμg/mL
Found conc.μg/mL
Recovery %
Claimed to be takenμg/mL
Authentic addedμg/mL
Found conc.μg/mL
Recovery %
15
14.99
99.93
15
14.94
99.59
7.5
7.56
100.80
10
10.05
100.48
15
14.89
99.24
15
15.04
100.28
7.5
7.46
99.52
10
10.14
101.44
20
19.86
99.30
20
20.02
100.08
10
10.10
100.96
12.5
12.39
99.14
22.5
22.50
100.01
25
25.04
100.17
15
15.12
100.80
15
14.98
99.84
25
25.20
100.79
30
29.91
99.71
17.5
17.27
98.70
17.5
17.46
99.79
27.5
27.74
100.86
32.5
32.61
100.33
20
20.14
100.72
20
20.05
100.24
30
29.76
99.19
35
34.83
99.53
22.5
22.44
99.73
22.5
22.68
100.80
35
35.15
100.41
40
40.12
100.30
25
25.17
100.67
27.5
27.56
100.22
37.5
37.58
100.21
42.5
42.55
100.13
27.5
27.56
100.22
30
30.05
100.16
40
39.65
99.19
30
29.95
99.84
Mean
99.91
100.01
100.20
100.23
Variance
0.44
0.10
0.54
0.42
SD
0.66
0.32
0.73
0.65
SE
0.15
0.11
0.24
0.23
*Average of three experiments.Table 3
Determination of azithromycin dihydrate in its pharmaceutical dosage forms through complexation with Cu(II) chloride and rose bengal by spectrophotometric procedure*.
Azrolid  tablets
Zithrokan capsules
Zithrokan  suspension
Claimed to be takenμg/mL
Authentic addedμg/mL
Found conc.μg/mL
Recovery %
Claimed to be takenμg/mL
Authentic addedμg/mL
Found conc.μg/mL
Recovery %
Claimed to be takenμg/mL
Authentic addedμg/mL
Found conc.μg/mL
Recovery %
4
4.03
100.77
4
4.01
100.36
4
4.05
101.18
4
4.01
100.36
4
4.08
101.99
4
4.05
101.18
7
7.01
100.19
5
4.97
99.42
6
6.06
100.95
8
8.00
100.02
6
6.02
100.41
8
8.02
100.22
9
8.96
99.53
8
7.97
99.62
10
10.06
100.60
10
10.03
100.28
9
9.06
100.61
12
11.99
99.91
11
11.11
101.03
10
10.00
99.95
14
14.01
100.10
12
11.97
99.77
11
10.98
99.85
16
15.89
99.34
14
14.03
100.22
12
12.02
100.18
16
15.89
99.34
13
12.99
99.95
Mean
100.15
100.23
100.43
Variance
0.27
0.51
0.43
SD
0.52
0.72
0.66
SE
0.17
0.24
0.25
*Average of three experiments.Table 4
Application of the proposed conductometric method to the determination of erythromycin thiocyanate in dosage forms*.
Erythrocin tablets
Erythrocin Pharco suspension
Taken (mg)
Found (mg)
Recovery %
Taken (mg)
Found (mg)
Recovery %
3
3.05
101.58
2
2
100
4
4
100
4
4.05
101.19
6
6.07
101.19
7
7.05
100.68
8
8.1
101.19
8
8.05
100.60
10
10
100
12
11.90
99.20
12
11.91
99.21
14
13.95
99.66
14
14.05
100.34
Mean
100.50
100.22
SD
0.85
0.73
SE
0.32
0.30
RSD
0.84
0.73
Variance
0.72
0.54
*Mean of three different experiments.Table 5
The intraday and interday precision and accuracy data for  the studied drugs using the spectrophotometric method.
Intraday
Interday
Drug
Taken (μg mL−1)
Found (μg mL−1)a
Recovery %
Precision RSD %b
Accuracy Er %c
Taken (μg mL−1)
Found (μg mL−1)a
Recovery %
Precision RSD %b
Accuracy Er %c
(I)
50
50.28
100.56
0.29
0.56
50
50.34
100.68
0.39
0.68
(II)
30
30.33
101.09
0.56
1.09
30
30.17
100.57
0.46
0.57
(III)
16
15.99
99.95
0.19
−0.05
16
16.01
100.07
0.25
0.07
aAverage of seven determinations.
bRSD %, percentage relative standard deviation.
cEr %, percentage relative error.Table 6
Analysis of clarithromycin 28μg mL−1 (5 × 10−4 M) by the spectrophotometric method in the presence of some common excipients.
Tolerance molar ratio ( M : M)*
Glucose
Sucrose
Carboxy methyl cellulose sodium
Sodium chloride
Recovery %**
1 : 1
101.24
101.07
100.89
99.82
1 : 10
100.36
101.24
99.11
99.82
1 : 50
115.45
102.13
100.35
99.47
1 : 100
106.57
107.46
98.22
99.29
*Drug: excipient.
**Mean of three determinations.Table 7
Determination of the studied drugs by the proposed spectrophotometric and conductmetric methods compared with the reported method [6].
(I)
(II)
(III)
Spectrophotometric method
Mean ± R.S.D.
100.05
±
0.54
100.01
±
0.47
99.87
±
0.50
Variance
0.29
0.22
0.25
Student’st-test
0.05 (2.17)*
0.4 (1.31)*
0.06 (2.16)*
F-test
2.38 (4.28)*
3.91 (3.60)*
2.68 (3.89)*
n
7
9
9
Conductometric method
Mean ± R.S.D.
100.66
±
0.93
100.65
±
0.63
100.62
±
0.62
Variance
0.87
0.40
0.38
Student’st-test
1.31 (2.17)*
1.13 (2.17)*
2.10 (2.228)*
F-test
1.26 (3.87)*
2.15 (3.97)*
1.76 (8.05)*
n
7
6
6
Reported method [6]
Mean ± R.S.D.
100.04
±
0.83
100.15 ± 0.93
99.89 ± 0.82
Variance
0.69
0.86
0.67
Student’st-test
—
—
—
F-test
—
—
—
n
7
8
6
*The figures in parenthesis are the theoretical values fort and F-tests (P<0.05).


## 3.1. Conditions for Spectrophotometric Method

### 3.1.1. Effect of pH

In a trial to elucidate the optimum medium for the quantitative determination of the studied drugs, the effect of different acetate and phosphate buffers of pH of range 2–10 was examined. It was found that buffer had no effect on the reaction.


### 3.1.2. Effect of Cu(II) Chloride Volume

The effect of Cu(II) chloride volume was also tested by using varying amounts (0.1–2 mL)  of Cu(II) chloride (3 mg/mL). The results showed that 0.2 mL, 1.5 mL, and 0.5 mL of Cu(II) chloride (3 mg/mL) for (I), (II), and (III), respectively, were sufficient for the production of maximum and reproducible colour intensity (Figure2).Figure 2
Effect of volume of 0.3% w/v Cu(II) chloride on the ternary complex formed with 40μg/mL erythromycin thiocyanate and 1.5 mL rose bengal 0.3% w/v; 25 μg/mL clarithromycin and 0.5 mL rose bengal 0.3% w/v; 13 μg/mL azithromycin dihydrate and 0.2 mL rose bengal 0.3% w/v.


### 3.1.3. Effect of Rose Bengal Volume

Highest constant absorbance was obtained on using 1.5 mL, 0.5 mL, and 0.2 mL of rose bengal (3 mg/mL) for (I), (II), and (III), respectively, (Figure3).Figure 3
Effect of volume of 0.3% w/v rose bengal on the ternary complex formed with 40μg/mL erythromycin thiocyanate and 0.2 mL Cu(II) chloride 0.3% w/v; 25 μg/mL clarithromycin and 1.5 mL Cu(II) chloride 0.3% w/v; 13 μg/mL azithromycin dihydrate and 0.5 mL Cu(II) chloride 0.3% w/v.


### 3.1.4. Effect of Order of Addition

The most suitable sequence was drug, Cu(II) chloride, and then rose bengal for all drugs for  the production of the highest colour intensity, while the other sequences produced lower absorbance values.


### 3.1.5. Effect of Reaction Time and Stability

Maximum colour intensity was attained immediately for the studied drugs except for (II); it needs 5 minutes to give complete complexation, and increasing time beyond this had no effect on absorption. The obtained colour was stable for at least 1 hour for all drugs at room temperature (25°C ± 2). The formation constants have been calculated by using the molar ratio method [32]. The formation constants (Kf) of the reaction product were found to be 7.13 × 104, 7.6 × 105, and 1.5 × 106 L·mol−1 for (I), (II), and (III), respectively. This high value indicates a very stable reaction product. The Gibbs free energy change of the reaction (ΔG) was also calculated and was found to be −2.78 × 104, −3.35 × 104, and −3.52 × 104 K·J·mole−1 for (I), (II), and (III), respectively. The negative value of ΔG points out to the spontaneous nature of the reaction [33].


### 3.1.6. Effect of the Extraction Time and Number of Extractions

Shaking the reaction mixture with the extracting organic solvent is required for complete extraction of the ternary complex formed. It was found that 1 minute is enough time for complete colour extraction. Complete extraction was attained by triple extraction with 3 × 3 mL of methylene chloride for all drugs.


### 3.1.7. Effect of Solvent

Many organic solvents were tried to extract the ternary complexes formed (chloroform, methylene chloride, ethylene chloride, benzene, toluene, and ethyl acetate). The results obtained indicated that methylene chloride is the appropriate solvent for extraction for all drugs.


### 3.1.8. Determination of Stoichiometric Balance Using Molar Ratio Method [34]

The reaction stoichiometry between erythromycin thiocyanate (I), clarithromycin (II), azithromycin dihydrate (III)  with Cu(II) chloride and rose bengal was studied by molar ratio method using an equimolar (2.5 × 10−4 M) solution of erythromycin thiocyanate (I), clarithromycin (II), and (1.25 × 10−4 M) solutions of azithromycin dihydrate (III), Cu(II) chloride, and rose bengal of the same molarities. The obtained results showed that the composition of the ternary complexes was 1 : 1 : 2 for erythromycin thiocyanate (I) and 1 : 2 : 2 for clarithromycin (II) and azithromycin dihydrate (III) : Cu(II) chloride: rose bengal (Figures 4, 5, and 6).Figure 4
Molar ratio method plot for 2.5 × 10−4 M erythromycin thiocyanate and 2.5 × 10−4 M Cu(II) chloride in presence of excess rose bengal (1.5 mL 0.3%); 2.5 × 10−4 M clarithromycin and 2.5 × 10−4 M Cu(II) chloride in presence of excess rose bengal (0.5 mL 0.3%); 1.25 × 10−4 M azithromycin dihydrate and 1.25 × 10−4 M Cu(II) chloride in presence of excess rose bengal (0.2 mL 0.3%) VR = Cu(II) chloride and Vd = Drug.Figure 5
Molar ratio method plot for 2.5 × 10−4 M erythromycin thiocyanate with 2.5 × 10−4 M rose bengal in presence of excess Cu(II) chloride (0.2 mL 0.3%); 2.5 × 10−4 M clarithromycin with 2.5 × 10−4 M rose bengal in presence of excess Cu(II) chloride (1.5 mL 0.3%); 1.25 × 10−4 M azithromycin dihydrate with 1.25 × 10−4 M rose bengal in presence of excess Cu(II) chloride (0.5 mL 0.3%). Vr  = rose bengal and Vd = Drug.Figure 6
Molar ratio method plot for 2.5 × 10−4 M rose bengal with 2.5 × 10−4 M Cu(II) chloride in presence of excess erythromycin thiocyanate (30 μg·mL−1); 2.5 × 10−4 M rose bengal with 2.5 × 10−4 M Cu(II) chloride in presence of excess clarithromycin (30 μg·mL−1); 1.25 × 10−4 M rose bengal with 1.25 × 10−4 M Cu(II) chloride in presence of excess azithromycin dihydrate (10 μg·mL−1). Vr = rose bengal and VR = Cu(II) chloride.Figure 7
Conductometric titration curve of 15 mg clarithromycin versus 3 × 103 M rose bengal.Scheme1 illustrates the mechanism of the reaction of clarithromycin, rose bengal and copper(II) chloride. The lactone ring in clarithromycin is substituted with a number of hydroxyl functional groups. These groups are positioned in suitable configuration for interaction with metal ions. So, copper cation forms a chelate with the lone pair of electrons of the two hydroxyl groups at C11 and C12. Also copper can form a chelate with the lone pair of electrons of the tertiary amine group nitrogen and the oxygen of the β-glycosidic linkage. So, the azithromycin-copper chelate bears an overall positive charge of the original two metal ions, that is, four positive charges. This cationic complex associates with two molecules of rose bengal anions to form the ternary complex.Scheme 1
Proposal Mechanism for the reaction between clarithromycin, rose bengal and copper(II) chloride. clarithromycin-Cu-rose bengal complex.


## 3.1.1. Effect of pH

In a trial to elucidate the optimum medium for the quantitative determination of the studied drugs, the effect of different acetate and phosphate buffers of pH of range 2–10 was examined. It was found that buffer had no effect on the reaction.


## 3.1.2. Effect of Cu(II) Chloride Volume

The effect of Cu(II) chloride volume was also tested by using varying amounts (0.1–2 mL)  of Cu(II) chloride (3 mg/mL). The results showed that 0.2 mL, 1.5 mL, and 0.5 mL of Cu(II) chloride (3 mg/mL) for (I), (II), and (III), respectively, were sufficient for the production of maximum and reproducible colour intensity (Figure2).Figure 2
Effect of volume of 0.3% w/v Cu(II) chloride on the ternary complex formed with 40μg/mL erythromycin thiocyanate and 1.5 mL rose bengal 0.3% w/v; 25 μg/mL clarithromycin and 0.5 mL rose bengal 0.3% w/v; 13 μg/mL azithromycin dihydrate and 0.2 mL rose bengal 0.3% w/v.


## 3.1.3. Effect of Rose Bengal Volume

Highest constant absorbance was obtained on using 1.5 mL, 0.5 mL, and 0.2 mL of rose bengal (3 mg/mL) for (I), (II), and (III), respectively, (Figure3).Figure 3
Effect of volume of 0.3% w/v rose bengal on the ternary complex formed with 40μg/mL erythromycin thiocyanate and 0.2 mL Cu(II) chloride 0.3% w/v; 25 μg/mL clarithromycin and 1.5 mL Cu(II) chloride 0.3% w/v; 13 μg/mL azithromycin dihydrate and 0.5 mL Cu(II) chloride 0.3% w/v.


## 3.1.4. Effect of Order of Addition

The most suitable sequence was drug, Cu(II) chloride, and then rose bengal for all drugs for  the production of the highest colour intensity, while the other sequences produced lower absorbance values.


## 3.1.5. Effect of Reaction Time and Stability

Maximum colour intensity was attained immediately for the studied drugs except for (II); it needs 5 minutes to give complete complexation, and increasing time beyond this had no effect on absorption. The obtained colour was stable for at least 1 hour for all drugs at room temperature (25°C ± 2). The formation constants have been calculated by using the molar ratio method [32]. The formation constants (Kf) of the reaction product were found to be 7.13 × 104, 7.6 × 105, and 1.5 × 106 L·mol−1 for (I), (II), and (III), respectively. This high value indicates a very stable reaction product. The Gibbs free energy change of the reaction (ΔG) was also calculated and was found to be −2.78 × 104, −3.35 × 104, and −3.52 × 104 K·J·mole−1 for (I), (II), and (III), respectively. The negative value of ΔG points out to the spontaneous nature of the reaction [33].


## 3.1.6. Effect of the Extraction Time and Number of Extractions

Shaking the reaction mixture with the extracting organic solvent is required for complete extraction of the ternary complex formed. It was found that 1 minute is enough time for complete colour extraction. Complete extraction was attained by triple extraction with 3 × 3 mL of methylene chloride for all drugs.


## 3.1.7. Effect of Solvent

Many organic solvents were tried to extract the ternary complexes formed (chloroform, methylene chloride, ethylene chloride, benzene, toluene, and ethyl acetate). The results obtained indicated that methylene chloride is the appropriate solvent for extraction for all drugs.


## 3.1.8. Determination of Stoichiometric Balance Using Molar Ratio Method [34]

The reaction stoichiometry between erythromycin thiocyanate (I), clarithromycin (II), azithromycin dihydrate (III)  with Cu(II) chloride and rose bengal was studied by molar ratio method using an equimolar (2.5 × 10−4 M) solution of erythromycin thiocyanate (I), clarithromycin (II), and (1.25 × 10−4 M) solutions of azithromycin dihydrate (III), Cu(II) chloride, and rose bengal of the same molarities. The obtained results showed that the composition of the ternary complexes was 1 : 1 : 2 for erythromycin thiocyanate (I) and 1 : 2 : 2 for clarithromycin (II) and azithromycin dihydrate (III) : Cu(II) chloride: rose bengal (Figures 4, 5, and 6).Figure 4
Molar ratio method plot for 2.5 × 10−4 M erythromycin thiocyanate and 2.5 × 10−4 M Cu(II) chloride in presence of excess rose bengal (1.5 mL 0.3%); 2.5 × 10−4 M clarithromycin and 2.5 × 10−4 M Cu(II) chloride in presence of excess rose bengal (0.5 mL 0.3%); 1.25 × 10−4 M azithromycin dihydrate and 1.25 × 10−4 M Cu(II) chloride in presence of excess rose bengal (0.2 mL 0.3%) VR = Cu(II) chloride and Vd = Drug.Figure 5
Molar ratio method plot for 2.5 × 10−4 M erythromycin thiocyanate with 2.5 × 10−4 M rose bengal in presence of excess Cu(II) chloride (0.2 mL 0.3%); 2.5 × 10−4 M clarithromycin with 2.5 × 10−4 M rose bengal in presence of excess Cu(II) chloride (1.5 mL 0.3%); 1.25 × 10−4 M azithromycin dihydrate with 1.25 × 10−4 M rose bengal in presence of excess Cu(II) chloride (0.5 mL 0.3%). Vr  = rose bengal and Vd = Drug.Figure 6
Molar ratio method plot for 2.5 × 10−4 M rose bengal with 2.5 × 10−4 M Cu(II) chloride in presence of excess erythromycin thiocyanate (30 μg·mL−1); 2.5 × 10−4 M rose bengal with 2.5 × 10−4 M Cu(II) chloride in presence of excess clarithromycin (30 μg·mL−1); 1.25 × 10−4 M rose bengal with 1.25 × 10−4 M Cu(II) chloride in presence of excess azithromycin dihydrate (10 μg·mL−1). Vr = rose bengal and VR = Cu(II) chloride.Figure 7
Conductometric titration curve of 15 mg clarithromycin versus 3 × 103 M rose bengal.Scheme1 illustrates the mechanism of the reaction of clarithromycin, rose bengal and copper(II) chloride. The lactone ring in clarithromycin is substituted with a number of hydroxyl functional groups. These groups are positioned in suitable configuration for interaction with metal ions. So, copper cation forms a chelate with the lone pair of electrons of the two hydroxyl groups at C11 and C12. Also copper can form a chelate with the lone pair of electrons of the tertiary amine group nitrogen and the oxygen of the β-glycosidic linkage. So, the azithromycin-copper chelate bears an overall positive charge of the original two metal ions, that is, four positive charges. This cationic complex associates with two molecules of rose bengal anions to form the ternary complex.Scheme 1
Proposal Mechanism for the reaction between clarithromycin, rose bengal and copper(II) chloride. clarithromycin-Cu-rose bengal complex.


## 3.2. Conditions for Conductometric Method

Investigations were carried out to establish the most suitable conditions for the ion associates formation of the cited drugs with rose bengal to attain sharp endpoint. The optimum conditions for performing the titration in a quantitative manner were elucidated as described later.


### 3.2.1. Reaction Medium

Titrations in different media were attempted to obtain the best results. Preliminary experiments were in:(i)
aqueous drug solution for (I) only (prepared by dissolving 100 mg drug in 2 mL methanol then completing to 100 mL with bidistilled water) with aqueous reagent solution,(ii)
ethanol drug solution with ethanol reagent solution,(iii)
drug solution with reagent solution, both in ethanol-water (50%, v/v) mixture,(iv)
methanol drug solution with methanol reagent solution (except for (II) as it is insoluble in methanol),(v)
drug solution with reagent solution, both in methanol-water mixture (50% v/v) (except for (II) as it is insoluble in methanol),(vi)
drug solution with reagent solution, both in acetone-water (50% v/v) mixture.Preliminary experiments showed that procedure in aqueous media was the most suitable for successful results for (I), 50% (v/v) ethanol for (II), and 50% (v/v) methanol for (III).


### 3.2.2. Reagent’s Concentration

Different concentrations of rose bengal solution were tried ranging from 2 × 10−2 to 5 × 10−4 molar solutions. The optimum concentration of the reagent was 3 × 10−3 M in titration of the studied drug to achieve a constant and highly stable conductance reading within 1-2 min of mixing. Concentrations less than these limits led to unstable readings, and more time was needed to obtain constant conductance values.Representative titration curve is shown in (Figure7). Two straight lines are obtained, intersecting at the endpoint; the first branch ascending, and the second has conductance values that would slightly increase after the equivalence point. The increase of conductance may be attributed to the formation of ion pair in solution as a result of the complexation reaction. After the end-point, the titration curves indicate a slightly increased value of conductance, despite the excess of the reagent. This may be due to further ionic condensation, leading to species of lower mobility.The conductometric titrations of different volumes of 3 × 10−3 M rose bengal solution were performed. The results show an obvious maximum in the conductance curve at drug-reagent molar ratio of 2 : 1 for (I) and (II) and 1 : 1 for (III). The reactions may be represented by (for (I))
(2)[RB]-2+2[ErythN(Me)2]→H+[RB]-2[ErythNH(Me)2]2+2
where [RB]−2 rose bengal anion [ErythN(Me)2] erythromycin thiocyanate [ErythNH(Me)2]+2 protonated erythromycin thiocyanate.Erythromycin thiocyanate tertiary amine nitrogen is protonated, so attraction occurs between it and rose bengal anion forming ion-pair complex.The conductance measured before the addition of the titrant (volume of rose bengal equals zero) is mainly due to the hydrogen ions mobility. These hydrogen ions result from two sources:(1)
the active methylene group in the drug structure as in Scheme2 which has the ability to make self protonation to the drug(2)
the protons coming from the alcohols used in solubility, as these alcohols have certain acidity character so can protonate the amino group in drug structure.Scheme 2
*The active methylene group in macrolide structure.So, there is no need for acidic buffer use for drug protonation. It was expected that the conductance values would remain constant or slightly increase after the equivalence point. However, the conductance of the solution decreased. This may be due to interaction of the protons available in the titration medium with the added reagent. The results from the conductometic titrations are summarized in Table4. The data show that accurate results were obtained with good recoveries and low standard deviation values. The optimum concentration ranges for determination of the cited drugs were in the range of 1–15 mg mL−1 for all drugs. At such ranges, sharp inflections (Figure 7) and stable conductance reading were obtained.


## 3.2.1. Reaction Medium

Titrations in different media were attempted to obtain the best results. Preliminary experiments were in:(i)
aqueous drug solution for (I) only (prepared by dissolving 100 mg drug in 2 mL methanol then completing to 100 mL with bidistilled water) with aqueous reagent solution,(ii)
ethanol drug solution with ethanol reagent solution,(iii)
drug solution with reagent solution, both in ethanol-water (50%, v/v) mixture,(iv)
methanol drug solution with methanol reagent solution (except for (II) as it is insoluble in methanol),(v)
drug solution with reagent solution, both in methanol-water mixture (50% v/v) (except for (II) as it is insoluble in methanol),(vi)
drug solution with reagent solution, both in acetone-water (50% v/v) mixture.Preliminary experiments showed that procedure in aqueous media was the most suitable for successful results for (I), 50% (v/v) ethanol for (II), and 50% (v/v) methanol for (III).


## 3.2.2. Reagent’s Concentration

Different concentrations of rose bengal solution were tried ranging from 2 × 10−2 to 5 × 10−4 molar solutions. The optimum concentration of the reagent was 3 × 10−3 M in titration of the studied drug to achieve a constant and highly stable conductance reading within 1-2 min of mixing. Concentrations less than these limits led to unstable readings, and more time was needed to obtain constant conductance values.Representative titration curve is shown in (Figure7). Two straight lines are obtained, intersecting at the endpoint; the first branch ascending, and the second has conductance values that would slightly increase after the equivalence point. The increase of conductance may be attributed to the formation of ion pair in solution as a result of the complexation reaction. After the end-point, the titration curves indicate a slightly increased value of conductance, despite the excess of the reagent. This may be due to further ionic condensation, leading to species of lower mobility.The conductometric titrations of different volumes of 3 × 10−3 M rose bengal solution were performed. The results show an obvious maximum in the conductance curve at drug-reagent molar ratio of 2 : 1 for (I) and (II) and 1 : 1 for (III). The reactions may be represented by (for (I))
(2)[RB]-2+2[ErythN(Me)2]→H+[RB]-2[ErythNH(Me)2]2+2
where [RB]−2 rose bengal anion [ErythN(Me)2] erythromycin thiocyanate [ErythNH(Me)2]+2 protonated erythromycin thiocyanate.Erythromycin thiocyanate tertiary amine nitrogen is protonated, so attraction occurs between it and rose bengal anion forming ion-pair complex.The conductance measured before the addition of the titrant (volume of rose bengal equals zero) is mainly due to the hydrogen ions mobility. These hydrogen ions result from two sources:(1)
the active methylene group in the drug structure as in Scheme2 which has the ability to make self protonation to the drug(2)
the protons coming from the alcohols used in solubility, as these alcohols have certain acidity character so can protonate the amino group in drug structure.Scheme 2
*The active methylene group in macrolide structure.So, there is no need for acidic buffer use for drug protonation. It was expected that the conductance values would remain constant or slightly increase after the equivalence point. However, the conductance of the solution decreased. This may be due to interaction of the protons available in the titration medium with the added reagent. The results from the conductometic titrations are summarized in Table4. The data show that accurate results were obtained with good recoveries and low standard deviation values. The optimum concentration ranges for determination of the cited drugs were in the range of 1–15 mg mL−1 for all drugs. At such ranges, sharp inflections (Figure 7) and stable conductance reading were obtained.


## 3.3. Method Validation

### 3.3.1. For the Spectrophotometric Method

Linearity. Under the optimum conditions described, standard calibration curves for erythromycin thiocyanate (I), clarithromycin (II), and azithromycin dihydrate (III) with rose bengal and copper were constructed by plotting absorbance against concentration. Conformity with Beer’s law was evident in the concentration range of the final dilution cited in Table 1. Beer’s law holds over the concentration ranges of 15–60, 7.5–40, and 4–20 μg·mL−1 for (I), (II), and (III), respectively. The linear regression equation for each drug was listed in Table 1. The correlation coefficient was 0.9999 indicating good linearity over the working concentration range.Sensitivity. The detection limit (LOD) for the proposed method was calculated using the following equation according to the ICH [35]:
(3)LOD=3.3σS,
where σ = the standard deviation of replicate blank responses (under the same conditions as for sample analysis), and  S = the slope of the calibration curve.The limits of quantification, LOQ, is defined as(4)LOQ=10σS.
According to the previous equations, the LODs and LOQs were calculated as in Table 1. Their values confirm the sensitivity of the proposed method.Accuracy and Precision. In order to determine the accuracy and precision of the proposed method, solutions containing one concentration of each drug were prepared and analysed in seven replicates. The relative standard deviation (RSD%) as precision and percentage relative error (Er%) as accuracy of the suggested method were calculated at 95% confidence levels and can be considered satisfactory. The percentage relative error was calculated according the following equation:
(5)Er%=[found-addedadded]×100.The inter- and intraday precisions and accuracy results are shown in Table5. The analytical results for accuracy and precision show that the proposed method has good repeatability and reproducibility.Interference. The effect of presence of common excipients such as glucose, sucrose, sodium chloride, carboxy methyl cellulose sodium, calcium carbonate, and magnesium stearate was studied. Table 6 presents the results of determination of clarithromycin as a representative example using the proposed procedure in the presence of aforementioned ingredients. The tolerance ratio was defined as the concentration which gave an error of ±3.0% in the determination of drugs. Results indicate that the tolerance ratio of drug excipient, (M : M), is 1 : 50 for glucose and 1 : 100 for sucrose. While there were no interference from the presence of sodium chloride, carboxy methyl cellulose sodium, calcium carbonate, and magnesium stearate. Interference due to glucose and sucrose was further investigated by studying their reaction with Cu(II) chloride and rose bengal. The study revealed that no interference could be observed at 557 nm, and an interaction product was obtained at λmax 498 in tolerance ratios 1 : 1 and 1 : 10 for glucose and 1 : 1, 1 : 10, 1 : 50 for sucrose. However, interference starts to appear at λmax 557 upon using 50 times, 100-fold excess of the molar concentration of clarithromycin for glucose and sucrose, respectively, as shown in Figure 8.Figure 8
Absorption spectra of (a) 2.5 × 10−2 M glucose with 1.5 mL Cu(II) chloride 0.3% w/v and 0.5 mL 0.3% w/v rose bengal, (b) 5 × 10−2 M sucrose with 1.5 mL Cu(II) chloride 0.3% w/v and 0.5 mL 0.3% w/v rose bengal.


### 3.3.2. For the Conductometric Method

In order to address the validity of the proposed method, statistical analysis of the data obtained from its application on the cited drugs in the pure form and in formulations was performed. The results revealed in Tables5 and 6 showed that the proposed method is satisfactorily accurate, precise, and reproducible over a concentration range of 1–15 mg. Results for the determination of the studied drugs using the previously mentioned method were compared with results from reported one [6], Table 7.


## 3.3.1. For the Spectrophotometric Method

Linearity. Under the optimum conditions described, standard calibration curves for erythromycin thiocyanate (I), clarithromycin (II), and azithromycin dihydrate (III) with rose bengal and copper were constructed by plotting absorbance against concentration. Conformity with Beer’s law was evident in the concentration range of the final dilution cited in Table 1. Beer’s law holds over the concentration ranges of 15–60, 7.5–40, and 4–20 μg·mL−1 for (I), (II), and (III), respectively. The linear regression equation for each drug was listed in Table 1. The correlation coefficient was 0.9999 indicating good linearity over the working concentration range.Sensitivity. The detection limit (LOD) for the proposed method was calculated using the following equation according to the ICH [35]:
(3)LOD=3.3σS,
where σ = the standard deviation of replicate blank responses (under the same conditions as for sample analysis), and  S = the slope of the calibration curve.The limits of quantification, LOQ, is defined as(4)LOQ=10σS.
According to the previous equations, the LODs and LOQs were calculated as in Table 1. Their values confirm the sensitivity of the proposed method.Accuracy and Precision. In order to determine the accuracy and precision of the proposed method, solutions containing one concentration of each drug were prepared and analysed in seven replicates. The relative standard deviation (RSD%) as precision and percentage relative error (Er%) as accuracy of the suggested method were calculated at 95% confidence levels and can be considered satisfactory. The percentage relative error was calculated according the following equation:
(5)Er%=[found-addedadded]×100.The inter- and intraday precisions and accuracy results are shown in Table5. The analytical results for accuracy and precision show that the proposed method has good repeatability and reproducibility.Interference. The effect of presence of common excipients such as glucose, sucrose, sodium chloride, carboxy methyl cellulose sodium, calcium carbonate, and magnesium stearate was studied. Table 6 presents the results of determination of clarithromycin as a representative example using the proposed procedure in the presence of aforementioned ingredients. The tolerance ratio was defined as the concentration which gave an error of ±3.0% in the determination of drugs. Results indicate that the tolerance ratio of drug excipient, (M : M), is 1 : 50 for glucose and 1 : 100 for sucrose. While there were no interference from the presence of sodium chloride, carboxy methyl cellulose sodium, calcium carbonate, and magnesium stearate. Interference due to glucose and sucrose was further investigated by studying their reaction with Cu(II) chloride and rose bengal. The study revealed that no interference could be observed at 557 nm, and an interaction product was obtained at λmax 498 in tolerance ratios 1 : 1 and 1 : 10 for glucose and 1 : 1, 1 : 10, 1 : 50 for sucrose. However, interference starts to appear at λmax 557 upon using 50 times, 100-fold excess of the molar concentration of clarithromycin for glucose and sucrose, respectively, as shown in Figure 8.Figure 8
Absorption spectra of (a) 2.5 × 10−2 M glucose with 1.5 mL Cu(II) chloride 0.3% w/v and 0.5 mL 0.3% w/v rose bengal, (b) 5 × 10−2 M sucrose with 1.5 mL Cu(II) chloride 0.3% w/v and 0.5 mL 0.3% w/v rose bengal.


## 3.3.2. For the Conductometric Method

In order to address the validity of the proposed method, statistical analysis of the data obtained from its application on the cited drugs in the pure form and in formulations was performed. The results revealed in Tables5 and 6 showed that the proposed method is satisfactorily accurate, precise, and reproducible over a concentration range of 1–15 mg. Results for the determination of the studied drugs using the previously mentioned method were compared with results from reported one [6], Table 7.


## 3.4. Analytical Applications

The proposed methods were applied to determine the studied drugs in their pharmaceutical dosage forms. Satisfactory results were obtained. The recovery of each drug was calculated by comparing the concentration obtained from the spiked mixtures with those of the drug. The results of analysis of the commercial dosage forms and the recovery study are shown in Tables2, 3, and 4. The results obtained were compared with the reported method [6]. No significant differences were found between the proposed methods and reported method. Statistical comparison of the results was performed using Student’s t-test and variance ratio F-test at 95% confidence level (Table 7).Table 2
Determination of erythromycin thiocyanate and clarithromycin in their pharmaceutical dosage forms through complexation with Cu(II) chloride and rose bengal by spectrophotometric procedure*.
Erythrocin tablets
Erythrocin Pharco suspension
Klarimix tablets
Klarimix suspension
Claimed to be takenμg/mL
Authentic addedμg/mL
Found conc.μg/mL
Recovery %
Claimed to be takenμg/mL
Authentic addedμg/mL
Found conc.μg/mL
Recovery %
Claimed to be takenμg/mL
Authentic addedμg/mL
Found conc.μg/mL
Recovery %
Claimed to be takenμg/mL
Authentic addedμg/mL
Found conc.μg/mL
Recovery %
15
14.99
99.93
15
14.94
99.59
7.5
7.56
100.80
10
10.05
100.48
15
14.89
99.24
15
15.04
100.28
7.5
7.46
99.52
10
10.14
101.44
20
19.86
99.30
20
20.02
100.08
10
10.10
100.96
12.5
12.39
99.14
22.5
22.50
100.01
25
25.04
100.17
15
15.12
100.80
15
14.98
99.84
25
25.20
100.79
30
29.91
99.71
17.5
17.27
98.70
17.5
17.46
99.79
27.5
27.74
100.86
32.5
32.61
100.33
20
20.14
100.72
20
20.05
100.24
30
29.76
99.19
35
34.83
99.53
22.5
22.44
99.73
22.5
22.68
100.80
35
35.15
100.41
40
40.12
100.30
25
25.17
100.67
27.5
27.56
100.22
37.5
37.58
100.21
42.5
42.55
100.13
27.5
27.56
100.22
30
30.05
100.16
40
39.65
99.19
30
29.95
99.84
Mean
99.91
100.01
100.20
100.23
Variance
0.44
0.10
0.54
0.42
SD
0.66
0.32
0.73
0.65
SE
0.15
0.11
0.24
0.23
*Average of three experiments.Table 3
Determination of azithromycin dihydrate in its pharmaceutical dosage forms through complexation with Cu(II) chloride and rose bengal by spectrophotometric procedure*.
Azrolid  tablets
Zithrokan capsules
Zithrokan  suspension
Claimed to be takenμg/mL
Authentic addedμg/mL
Found conc.μg/mL
Recovery %
Claimed to be takenμg/mL
Authentic addedμg/mL
Found conc.μg/mL
Recovery %
Claimed to be takenμg/mL
Authentic addedμg/mL
Found conc.μg/mL
Recovery %
4
4.03
100.77
4
4.01
100.36
4
4.05
101.18
4
4.01
100.36
4
4.08
101.99
4
4.05
101.18
7
7.01
100.19
5
4.97
99.42
6
6.06
100.95
8
8.00
100.02
6
6.02
100.41
8
8.02
100.22
9
8.96
99.53
8
7.97
99.62
10
10.06
100.60
10
10.03
100.28
9
9.06
100.61
12
11.99
99.91
11
11.11
101.03
10
10.00
99.95
14
14.01
100.10
12
11.97
99.77
11
10.98
99.85
16
15.89
99.34
14
14.03
100.22
12
12.02
100.18
16
15.89
99.34
13
12.99
99.95
Mean
100.15
100.23
100.43
Variance
0.27
0.51
0.43
SD
0.52
0.72
0.66
SE
0.17
0.24
0.25
*Average of three experiments.Table 4
Application of the proposed conductometric method to the determination of erythromycin thiocyanate in dosage forms*.
Erythrocin tablets
Erythrocin Pharco suspension
Taken (mg)
Found (mg)
Recovery %
Taken (mg)
Found (mg)
Recovery %
3
3.05
101.58
2
2
100
4
4
100
4
4.05
101.19
6
6.07
101.19
7
7.05
100.68
8
8.1
101.19
8
8.05
100.60
10
10
100
12
11.90
99.20
12
11.91
99.21
14
13.95
99.66
14
14.05
100.34
Mean
100.50
100.22
SD
0.85
0.73
SE
0.32
0.30
RSD
0.84
0.73
Variance
0.72
0.54
*Mean of three different experiments.Table 5
The intraday and interday precision and accuracy data for  the studied drugs using the spectrophotometric method.
Intraday
Interday
Drug
Taken (μg mL−1)
Found (μg mL−1)a
Recovery %
Precision RSD %b
Accuracy Er %c
Taken (μg mL−1)
Found (μg mL−1)a
Recovery %
Precision RSD %b
Accuracy Er %c
(I)
50
50.28
100.56
0.29
0.56
50
50.34
100.68
0.39
0.68
(II)
30
30.33
101.09
0.56
1.09
30
30.17
100.57
0.46
0.57
(III)
16
15.99
99.95
0.19
−0.05
16
16.01
100.07
0.25
0.07
aAverage of seven determinations.
bRSD %, percentage relative standard deviation.
cEr %, percentage relative error.Table 6
Analysis of clarithromycin 28μg mL−1 (5 × 10−4 M) by the spectrophotometric method in the presence of some common excipients.
Tolerance molar ratio ( M : M)*
Glucose
Sucrose
Carboxy methyl cellulose sodium
Sodium chloride
Recovery %**
1 : 1
101.24
101.07
100.89
99.82
1 : 10
100.36
101.24
99.11
99.82
1 : 50
115.45
102.13
100.35
99.47
1 : 100
106.57
107.46
98.22
99.29
*Drug: excipient.
**Mean of three determinations.Table 7
Determination of the studied drugs by the proposed spectrophotometric and conductmetric methods compared with the reported method [6].
(I)
(II)
(III)
Spectrophotometric method
Mean ± R.S.D.
100.05
±
0.54
100.01
±
0.47
99.87
±
0.50
Variance
0.29
0.22
0.25
Student’st-test
0.05 (2.17)*
0.4 (1.31)*
0.06 (2.16)*
F-test
2.38 (4.28)*
3.91 (3.60)*
2.68 (3.89)*
n
7
9
9
Conductometric method
Mean ± R.S.D.
100.66
±
0.93
100.65
±
0.63
100.62
±
0.62
Variance
0.87
0.40
0.38
Student’st-test
1.31 (2.17)*
1.13 (2.17)*
2.10 (2.228)*
F-test
1.26 (3.87)*
2.15 (3.97)*
1.76 (8.05)*
n
7
6
6
Reported method [6]
Mean ± R.S.D.
100.04
±
0.83
100.15 ± 0.93
99.89 ± 0.82
Variance
0.69
0.86
0.67
Student’st-test
—
—
—
F-test
—
—
—
n
7
8
6
*The figures in parenthesis are the theoretical values fort and F-tests (P<0.05).


## 4. Conclusions

The proposed methods are advantageous when compared to many of the reported spectrophotometric methods in having higher sensitivity. The data given previously reveal that the proposed methods are simple, accurate, and sensitive with good precision and accuracy. With these methods, one can do the analysis at low cost without losing accuracy. No interference from excipients was encountered. Also, In comparison with the chromatographic methods, the proposed methods are advantageous as HPLC and GLC are not available especially in developing countries, and they require complex and expensive equipment, intensive sample preparation, and personnel skilled in chromatographic techniques. So, the proposed methods can be used as alternative methods to the reported ones for the routine determination of erythromycin thiocyanate (I), clarithromycin (II), azithromycin dihydrate (III) in the pure form and in pharmaceutical formulations depending upon the availability of chemicals and the equipment.



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*Source: 214270-2013-01-20.xml*