# Antistaphylococcal and Antibiotic Resistance Modulatory Activities of Thirteen Cameroonian Edible Plants against Resistant Phenotypes

Authors: Brice E. N. Wamba; Armelle T. Mbaveng; Paul Nayim; Joachim K. Dzotam; Ornella J. T. Ngalani; Victor Kuete
Journal: International Journal of Microbiology
 (2018)

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## Abstract

Background. In this study, 18 methanol extracts from Cameroonian edible plants were tested for their antibacterial activities against 26 strains of S. aureus; the role of efflux pumps in the resistance of tested bacteria and the antibiotic resistance-modulating activities against selected multidrug-resistant (MDR) phenotypes were also investigated. Methods. Broth microdilution assay was used to evaluate the antibacterial activity, the role of efflux pumps, and the antibiotic resistance-modulating effects of plant extracts. Results. Extracts from Dacryodes edulis seeds (DES) and Dacryodes edulis bark (DEB) were active against all 26 tested bacterial strains, within the minimal inhibitory concentration (MIC) range of 256–1024 µg/mL. MIC values varied from 64 to 1024 µg/mL against 96.2% of the 26 tested bacteria for Phaseolus vulgaris leaves (PVL), 92.3% for Azadirachta indica bark (AIB), Dacryodes edulis leaves (DEL), and Ricinodendron heudelotii leaves (RHL). The lowest MIC value of 64 µg/mL was obtained with the extract from Cucurbita maxima beans (CMB) against MRSA4 strain and from Uapaca guineensis bark (UGB) against MRSA9 strain. Bacterial efflux pump inhibitor (EPI), carbonyl cyanide m-chlorophenyl hydrazone (CCCP), improved the activity of DES and UGB as well as that of extracts from Hibiscus esculentus leaves (HEL) and Uapaca guineensis leaves (UGL) against resistant S. aureus strains. Antibiotic-modulating effects against more than 70% of the S. aureus strains tested were obtained when RHL (at MIC/2) was combined with CIP, ERY, and KAN (88.89%), CHL (88.89%), TET (77.78%), and STR (88.89%). Conclusion. The present study demonstrated that the 13 tested plants had antistaphylococcal effects and that DES, HEL, UGL, and UGB could be used in combination with EPI to combat resistance to Staphylococcus aureus. Also, it demonstrated that some studied extracts and mostly RHL could be used as antibiotic resistance modulators to fight against resistant strains of S. aureus.


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## Body

## 1. Background

Bacterial infections caused byStaphylococcus aureus are globally responsible for 7–10% of deaths annually [1]. It is the most virulent species of the genus Staphylococcus and has emerged as one of the most important human pathogens in the last decades, being one of the main causes of hospital and community infections [2]. This bacterium causes a wide range of clinical infections, ranging from common infections such as skin and soft tissue infections to septicemia, pneumonia, and toxinosis [3, 4]. The fight against S. aureus is hindered by the development of resistance of various strains to antibiotics [5–9]. The multidrug resistance (MDR) observed in Gram-positive bacteria is mostly attributed to overexpression of efflux pumps and antibiotics-degrading enzymes. This MDR of S. aureus propels the search of new antibacterials with more efficiency and low toxicity. Plant kingdom contains a variety of pharmacologically active secondary metabolites, and some of them have been reported for their antibacterial activities [10, 11]. Their use to combat S. aureus antibiotic resistance is an attractive strategy. In regard to the loss of efficacy of several antibiotics and the scarcity of new antibacterial agents, it is also important to search for substances capable of restoring the activity of antibiotics. Antibacterial screenings of African plants have yielded promising results in the past [12–15]. The present study was set up to evaluate the antistaphylococcal potential of 13 Cameroonian food plants: Azadirachta indica A. Juss (Meliaceae), Citrus grandis (L.) Osbeck (Rutaceae), Cucurbita maxima Duch. (Cucurbitaceae), Dacryodes edulis (G. Don) H. J. Lam (Burseraceae), Hibiscus esculentus L. (Malvaceae), Ipomoea batatas (L.) Lam (Convolvulaceae), Irvingia gabonensis (Aubry. Lec. ex O. Rorke) Baill. (Irvingiaceae), Phaseolus vulgaris L. (Fabaceae), Ricinodendron heudelotii (Baill.) Pierre ex Heckel (Euphorbiaceae), Saccharum officinarum L. (Poaceae), Spondias mombin L. (Anacardiaceae), Theobroma cacao L. (Sterculiaceae), and Uapaca guineensis Muell. Arg. (Euphorbiaceae). The study was extended on the role of efflux pumps in resistance to some plant extracts as well as the ability of extracts to potentiate the activity of selected antibiotics.


## 2. Materials and Methods

### 2.1. Plant Materials and Extraction

The thirteen edible plants were collected from different localities of Cameroon, namely, Obala (Centre Region), Muyuka (South-West Region), and Dschang (West Region) from March to April 2016. The collected plant samples included the bark ofAzadirachta indica A. Juss (Meliaceae), leaves of Citrus grandis (L.) Osbeck (Rutaceae), beans of Cucurbita maxima Duch. (Cucurbitaceae), leaves, bark, and seeds of Dacryodes edulis (G. Don) H. J. Lam (Burseraceae), leaves of Hibiscus esculentus L. (Malvaceae), beans of Ipomoea batatas (L.) Lam (Convolvulaceae), leaves of Irvingia gabonensis (Aubry. Lec. ex O. Rorke) Baill. (Irvingiaceae), leaves of Phaseolus vulgaris L. (Fabaceae), bark and leaves of Ricinodendron heudelotii (Baill.) Pierre ex Heckel (Euphorbiaceae), leaves of Saccharum officinarum L. (Poaceae), leaves of Spondias mombin L. (Anacardiaceae), leaves and beans of Theobroma cacao L. (Sterculiaceae), and leaves and bark of Uapaca guineensis Muell. Arg. (Euphorbiaceae). Plants were identified at the National Herbarium in Yaoundé (Cameroon) where the voucher specimens were conserved under the registration numbers (Table 1). The dried and powdered material (100 g) of each plant was macerated in 300 mL of methanol at room temperature for 48 h and then filtered using Whatman filter paper No. 1. The filtrate obtained was concentrated using a rotary evaporator under reduced pressure to obtain the crude methanol extract, which was kept at 4°C until further use.Table 1
Information on the studied plants.
Species (family); voucher number
Traditional uses
Bioactive or potentially bioactive components
Known antimicrobial activities of plants
Azadirachta indica A. Juss (Meliaceae); 4447/SRFK
Antimalarial, anticancer, anti-inflammatory, antidiabetic, antihyperglycaemic, antiulcerous [16]
Alkaloids, glycosides, flavonoids, and saponins [17]
Antibacterial activity of ethanol leaf extract:Ec, Kp, Pm, Sa, Pa, Ef [18]; ethanol and methanol extract of leaves: Ec, Pa, St, Sa, Bp [17]
Citrus grandis (L.) Osbeck (Rutaceae); 25,860/SRFC
Cancer [19, 20]
Alkaloids, cardioglycosides, saponins, tannins, terpenoids, flavonoids, and steroids [21]
Antibacterial activity of petroleum ether, ethyl acetate, chloroform, ethanol, and leaf water:Ec, Sa, Pa, Pm [21]
Cucurbita maxima Duch. (Cucurbitaceae); 42,449/HNC
Diabetes, cancer, antihypertensive, anti-inflammatory, immunomodulating, and bacterial infections [22, 23]
Tannins, saponins, alkaloids [24], cucurbitaxanthin, gibberellin, and α-tocopherol [25]
Antibacterial activity of aqueous seed extract:Ec, Sa, Kp, Ef, Pa [24], ethanol and aqueous extract of flowers: St, Ec, Ef, Bc [26]
Dacryodes edulis (G. Don) H. J. Lam (Burseraceae); 1874/SRFK
Gastrointestinal disorders, toothache, earache [27], dysentery, anemia, leprosy, [28], skin diseases, and sickle cell disease [29]
Ethyl gallate and quercitrin [27]
Antibacterial activity of hydromethanolic, butanol, aqueous, ethanolic extract, ethyl acetate of bark:Ec, Pa, Bc, Sa [27]
Hibiscus esculentus L. (Malvaceae); 42,823/HNC
Inflammation, pain [30], cancer, hypoglycemic [31]
Not reported
Not reported
Ipomoea batatas (L.) Lam (Convolvulaceae); 55,594/HNC
Antidiabetic, anti-inflammatory, reducing risk of cardiovascular disease, anticancer, reducing stomach stress, nausea, diarrhea [32]
Caffeoylquinic acid [33], vitamin E, beta-carotene, lutein, saponins [32], flavonoids, and chitinases [34]
Not reported
Irvingia gabonensis (Aubry. Lec. ex O. Rorke) Baill. (Irvingiaceae); 52,936/HNC
Diarrhea, hernia, yellow fever, dysentery, antipoison [35, 36], gonorrhea, gastrointestinal and hepatic disorders, wound infections, diabetes, analgesic [37]
Saponins, tannins, phenols, and phlobatannins [35], alkaloids, cardiac glycosides, anthraquinones, tannins, flavonoids [36], 3-friedelanone; betulinic acid; oleanolic acid; 3,3,4-tri-O-methylellagic acid; 3,4-di-O-methylellagic acid; hardwickiic acid [38]
Antibacterial activity of aqueous and ethanol extract of leaves and bark:Sa, Ec [36]; S: Bst, Ca, Cf, Ea, Ecl, Mm, Ng, Pa, Pm, Pv, Sa, Sd; Bc, Bm, Bs, Ck, Ec, Kp, Sfl, St, Sf [38]
Phaseolus vulgaris L. (Fabaceae); 42,587/HNC
Antioxidant [39], cancer, estrogenic, antidepressant [40], bacterial infections, tuberculosis [41]
Alkaloids, steroids, and flavonoids [42]
Antibacterial activity of aqueous, alcohol, chloroform, ether extract of seeds:Sa, Pa, Sf, Bs, Kp, Ec [41]
Ricinodendron heudelotii (Baill.) Pierre ex Heckel (Euphorbiaceae); 50,852/HNC
Cough, antidote, intestinal diseases [43], yellow fever, malaria, stomach pain, headache, dysentery [44]
Tannins, polyphenols, alkaloids, glycosides, flavonoids, steroids, and saponins [43], aleuritolic acid, labda-8(17), 13-diem-3β, 15-diol, E-ferulic acid octacosylate [44]
Antibacterial activity of methanol extract of the leaves:Sa, Sf, Pv, Cf, Mm, Ko, Kp, Ec, Pa, St [43], Pf, Bs, Sa, Ec, Ca, Af [44], Ec, Ea, Pa, Ps, Kp, Ecl [18]
Saccharum officinarum L. (Poaceae); 42,958/HNC
Jaundice and liver problems, hemorrhoids, dysentery, menorrhagia [45]
Flavonoids, saponins, tannins, and alkaloids [46]
Antibacterial activity of methanol extract of the stems:Ec, Kp, Sa, Pa [46]
Spondias mombin L. (Anacardiaceae); 21,249/SRFK
Diuretic, febrifuge, diarrheal diseases, dysentery, hemorrhoids, gonorrhea, leucorrhea [47]
Saponins, tannins, flavonoids, alkaloids, and glycosides [48]
Antibacterial activity of ethanol, methanol, water, and acetone extracts of the leaves:Kp, Sa, St, Ea, Sm [48]
Theobroma cacao L. (Sterculiaceae); 60,111/HNC
Cardiovascular, gastrointestinal, and nervous diseases [49]
Alkaloids, anthraquinones, cardiac glycosides, and saponins [50]
Antibacterial activity:Sa, Ec, Sd, Kp, Sm, Pa, Pm [51]
Uapaca guineensis Muell. Arg. (Euphorbiaceae); 53,136/HNC
Leprosy, epilepsy, edema, rheumatism, aphrodisiac, fever, inflammation, absorption [52]
Steroids, alkaloid, terpenoids, and gallic acid [53]
Not reported
HNC: Herbier National du Cameroun; SRFC: Société des Réserves Forestières du Cameroun; SRFK: Société des Réserves forestières du Kamerun; Af:Aspergillus flavus; Bp: Bacillus pumilus; Bc: Bacillus cereus; Bm: Bacillus megaterium; Bs: Bacillus subtilis; Ca: Candida albicans; Cf; Citrobacter freundii; Ck: Candida krusei; Ea: Enterobacter aerogenes; Ec: Escherichia coli; Ecl: Enterobacter cloacae; Ef: Enterococcus faecium; Kp: Klebsiella pneumoniae; Ko: Klebsiella oxytoca; Mm: Morganella morganii; Ng: Neisseria gonorrhoeae; Pa: Pseudomonas aeruginosa; Pf: Pseudomonas fluorescens; Pm: Proteus mirabilis; Ps: Providencia stuartii; Pv: Proteus vulgaris; Sa: Staphylococcus aureus; Sd: Shigella dysenteriae; Sf; Streptococcus faecalis; Sf l: Shigella flexneri; Sm: Serratia marcescens; St: Salmonella typhi.


### 2.2. Chemicals

Eight reference antibiotics (RA) purchased from Sigma-Aldrich (St Quentin Fallavier, France) were tested: ampicillin (AMP), cefepime (CEF), chloramphenicol (CHL), ciprofloxacin (CIP), erythromycin (ERY), kanamycin (KAN), streptomycin (STR), and tetracycline (TET);p-iodonitrotetrazolium chloride (INT) (Sigma-Aldrich) was used as bacterial growth revelator; carbonyl cyanide m-chlorophenyl hydrazone (CCCP) and chlorpromazine (CPZ) (Sigma-Aldrich) were used as efflux pump inhibitors (EPIs); and dimethylsulfoxide (DMSO) was used to dissolve the plant extracts.


### 2.3. Bacteria, Culture Media, and Growth Conditions

The strains ofStaphylococcus aureus used included a reference strain obtained from American Type Culture Collection (ATCC; ATCC 25923), 8 methicillin-resistant S. aureus (MRSA) strains (MSSA1, MRSA3, MRSA4, MRSA6, MRSA8, MRSA9, MRSA11, and MRSA12) (obtained from the culture collection of the Laboratory of Microbiology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Japan, and provided by Dr Jean P. Dzoyem, University of Dschang) [8, 9], and 17 resistant clinical laboratory strains of S. aureus (SA01, SA07, SA18, SA23, SA36, SA39, SA56, SA64, SA68, SA88, SA114, SA116, SA124, SA126, SA127, SA135, and SA139) available in our laboratory collection and previously isolated from patients in Ad-Lucem Hospital in Banka-Bafang (West Region of Cameroon) [54]. Their bacterial features are summarized in Table 2. They were cultured at 37°C overnight on Mueller Hinton agar 24 h prior to any assay. The Mueller Hinton broth (MHB) was used as liquid culture medium for susceptibility tests.Table 2
Bacterial strains and features.
Bacteria
Features
References
ATCC 25923
Reference strain
—
S. aureus MSSA1
Clinical isolate: Met susceptible; Nisr, Chlr
[8, 9]
S. aureus MRSA3
Clinical isolate: Ofxar, Kanr, Tetr, Ermr
[8]
S. aureus MRSA4
Clinical isolate: Ofxar, Kanr, Cypr, Chlr, Genr, Nisr, Ampr
[8, 9]
S. aureus MRSA6
Clinical isolate: Ofxar, Flxr, Kanr, Tetr, Cypr, IM/Csr, Chlr, Genr, Nisr, Ampr
[8, 9]
S. aureus MRSA8
Clinical isolate: Ofxar, Flxr, Kanr, Ermr, Cypr, Im/Csr, Chlr, Genr, Nisr, Ampr
[8, 9]
S. aureus MRSA9
Clinical isolate: Ofxar, Flxr, Tetr, Ermr, Cypr, Im/Csr, Chlr, Genr, Nisr, Ampr
[8, 9]
S. aureus MRSA11
Clinical isolate: Ofxar, Kanr, Ermr, Cypr, Im/Csr, Chlr, Nisr, Ampr
[8, 9]
S. aureus MRSA12
Clinical isolate: Ofxar, Flxr, Kanr, Ermr, Im/Csr, Chlr, Genr, Nisr, Ampr
[8, 9]
SA01
Clinical isolate: Ermr, Ampr
[54]
SA07
Clinical isolate: Ermr, Doxr
[54]
SA18
Clinical isolate: Ampr, Doxr, Vmr
[54]
SA23
Clinical isolate: Imir, Augr
[54]
SA36
Clinical isolate: Doxr, Vmr
[54]
SA39
Clinical isolate: Ampr
[54]
SA56
Clinical isolate: Ampr, Doxr
[54]
SA64
Clinical isolate: Ampr, Doxr
[54]
SA68
Clinical isolate: Ampr, Vmr
[54]
SA88
Clinical isolate: Ermr, Vmr
[54]
SA114
Clinical isolate: Ampr, Doxr
[54]
SA116
Clinical isolate: Ermr
[54]
SA124
Clinical isolate: Ermr
[54]
SA126
Clinical isolate: Ampr, Doxr
[54]
SA127
Clinical isolate: Ampr, Doxr
[54]
SA135
Clinical isolate: Ermr
[54]
SA139
Clinical isolate: Ermr
[54]
Chlr, Cypr, Ermr, Flxr, Im/Csr, Kanr, Metr, Ofxar, Tetr, Vmr, Ampr, Doxr, Augr, Genr, and Nisr resistance to chloramphenicol, ciprofloxacin, erythromycin, flomoxef, imipenem/cilastatin sodium, kanamycin, methicillin, ofloxacin, tetracycline, vancomycin, ampicillin, doxycycline, augmentin, gentamicin, and nisin, respectively, SA: Staphylococcus aureus.


### 2.4. Preliminary Phytochemical Screenings

Potential classes of potential antibacterial phytochemicals such as alkaloids (Dragendorff’s and Mayer’s tests: 5 mg plant extract in 10 mL methanol; a portion of 2 mL extract + 1% HCl + steam, 1 mL filtrate + 6 drops of Mayor’s reagents/Wagner’s reagent/Dragendorff’s reagent; cream precipitate/brownish-red precipitate/orange precipitate indicated the presence of respective alkaloids), saponins (frothing test: 0.5 mL filtrate+ 5 mL distilled water; frothing persistence indicated the presence of saponins), steroids and triterpenoids (Liebermann–Burchard test: 5 mg plant extract in 10 mL chloroform, filtered; a 2 mL filtrate + 2 mL acetic anhydride+ conc. H2SO4; blue-green ring or pink-purple indicated the presence of steroids or triterpenoids), phenolics: anthraquinones (5 mg plant extract in 10 mL methanol; a portion of 2 mL + 2 mL ether-chloroform 1 : 1 v/v + 4 mL NaOH 10% (w/v); red color indicated the presence of anthraquinones), flavonoids (5 mg plant extract in 10 mL methanol; a portion of 2 mL + conc. HCl + magnesium; ribbon pink-tomato red color indicated the presence of flavonoids), polyphenols (ferric chloride test: 5 mg plant material in 10 mL methanol; a portion of 2 mL + 2 mL FeCl3; violet-blue or greenish color indicated the presence of phenols), and tannins (5 mg plant extract in 10 mL distilled water; a portion of 2 mL + 2 mL FeCl3; blue-black precipitate indicated the presence of tannins) (Table 3) were investigated according to the described phytochemical methods [11, 55].Table 3
Extraction yields and phytochemical composition of the plant extracts.
Plant extract and part used
Yields (%)
Alkaloids
Polyphenols
Flavonoids
Anthraquinones
Tannins
Triterpenes
Steroids
Saponins
Azadirachta indica
Bark
10.3
+
+
−
−
+
−
+
+
Citrus grandis
Leaves
2.6
+
+
−
−
+
+
+
−
Cucurbita maxima
Beans
2.6
−
+
−
−
+
+
+
+
Dacryodes edulis
Leaves
6.2
−
+
+
+
+
+
+
+
Bark
9.1
−
+
−
+
+
+
+
+
Seeds
6.9
−
+
+
+
+
+
+
+
Hibiscus esculentus
Leaves
1.9
−
+
−
−
+
−
+
−
Ipomoea batatas
Beans
3.3
+
+
+
+
+
+
−
+
Irvingia gabonensis
Leaves
6.7
−
+
−
−
+
−
+
+
Phaseolus vulgaris
Leaves
1.2
−
+
−
−
+
−
+
+
Ricinodendron heudelotii
Bark
2.9
−
+
+
+
+
+
+
−
Leaves
7.2
−
+
+
+
+
+
+
+
Spondias mombin
Leaves
21.4
−
+
−
−
+
+
+
−
Saccharum officinarum
Leaves
8.4
−
+
−
−
+
−
+
+
Theobroma cacao
Leaves
3.1
−
+
−
−
+
+
+
+
Beans
6.2
+
+
+
+
+
+
+
+
Uapaca guineensis
Leaves
7.3
−
+
−
−
+
+
+
+
Bark
6.1
+
+
−
−
+
+
+
+
−: absent; +: present; yield calculated as the ratio of the mass of the obtained methanol extract/mass of the plant powder.


### 2.5. INT Colorimetric Assay for MIC and Minimal Bactericidal Concentration (MBC) Determinations

The MIC and minimal bactericidal concentration (MBC) determinations on various strains ofS. aureus were performed using the rapid INT colorimetric assay [56] with some modifications as previously described [14, 38]. The samples were dissolved in DMSO/MHB. The final concentration of DMSO was lower than 2.5%. The twofold dilutions of the samples were made in a 96-well microplate, and the tested bacterial concentration was 1.5 × 106 colony-forming unit (CFU)/mL. The microplates were incubated at 37°C for 18 h. All assays were performed in triplicate and repeated thrice. Wells containing MHB, 100 µL of inoculum, and DMSO to a final concentration of 2.5% served as negative control. The MIC of each sample was detected after 18 h incubation at 37°C, following addition (40 µL) of 0.2 mg/mL of INT and incubation at 37°C for 30 minutes as the lowest sample concentration that prevented the color change of the medium and exhibited complete inhibition of microbial growth [56]. The MBC was determined by adding 50 µL aliquots of the preparations, which did not show any growth after incubation during MIC assays, to 150 µL of MHB. These preparations were further incubated at 37°C for 48 h. The MBC was regarded as the lowest concentration of samples, which did not produce a color change after addition of INT as mentioned above [57, 58].


### 2.6. Evaluation of the Role of Efflux Pumps in the Resistance of Selected Bacteria

To evaluate the involvement of efflux pumps in the resistance of selected bacterial strains to some of the active plant extracts,Dacryodes edulis seeds (DES), Hibiscus esculentus leaves (HEL), Uapaca guineensis leaves (UGL), Uapaca guineensis bark (UGB), and CIP (reference drug) were tested in the absence or presence of EPI (CCCP (0.5 µg/mL) or CPZ (25 µg/mL)). MICs of samples alone or in combination with EPI were determined as above, and the increase in activity was determined as the ratio of MIC of sample alone versus sample in combination with EPI. All assays were performed in triplicate and repeated thrice.


### 2.7. Antibiotic Activity Modulation Assays

To evaluate the antibiotic resistance modulation activity of the most active extracts:Azadirachta indica bark (AIB), Dacryodes edulis seeds (DES), Dacryodes edulis bark (DEB), Dacryodes edulis leaves (DEL), Phaseolus vulgaris leaves (PVL), Ricinodendron heudelotii leaves (RHL), and Uapaca guineensis bark (UGB), a preliminary assay was performed in order to assess the MICs of antibiotics in the absence and presence of these extracts using the broth microdilution method as previously described [14, 38, 56]. S. aureus SA88 was used for the preliminary assay, and the samples were tested at various subinhibitory concentrations (MIC/2, MIC/4, MIC/8, and MIC/16). Results allowed to select DEB, DEL, DES, RHL, and UGB to be tested further against S. aureus ATCC 25923, and 8 resistant strains of S. aureus (MRSA3, MRSA4, MRSA9, MRSA11, MRSA12, SA18, SA36, and SA64) at MIC/2 and MIC/4. Briefly, after serial dilutions of antibiotics, extract was added to each well at its subinhibitory concentrations, the bacterial inoculation was done, and the MIC was determined. Rows receiving antibiotic dilutions without extracts were used for the determination of the MICs of the antibiotics. The modulation factor was defined as the ratio of the MIC of antibiotic alone versus that of antibiotic in the presence of extract. Modulation factor ≥2 was set as the cutoff for biologically significance of antibiotic resistance-modulating effects [59].


## 2.1. Plant Materials and Extraction

The thirteen edible plants were collected from different localities of Cameroon, namely, Obala (Centre Region), Muyuka (South-West Region), and Dschang (West Region) from March to April 2016. The collected plant samples included the bark ofAzadirachta indica A. Juss (Meliaceae), leaves of Citrus grandis (L.) Osbeck (Rutaceae), beans of Cucurbita maxima Duch. (Cucurbitaceae), leaves, bark, and seeds of Dacryodes edulis (G. Don) H. J. Lam (Burseraceae), leaves of Hibiscus esculentus L. (Malvaceae), beans of Ipomoea batatas (L.) Lam (Convolvulaceae), leaves of Irvingia gabonensis (Aubry. Lec. ex O. Rorke) Baill. (Irvingiaceae), leaves of Phaseolus vulgaris L. (Fabaceae), bark and leaves of Ricinodendron heudelotii (Baill.) Pierre ex Heckel (Euphorbiaceae), leaves of Saccharum officinarum L. (Poaceae), leaves of Spondias mombin L. (Anacardiaceae), leaves and beans of Theobroma cacao L. (Sterculiaceae), and leaves and bark of Uapaca guineensis Muell. Arg. (Euphorbiaceae). Plants were identified at the National Herbarium in Yaoundé (Cameroon) where the voucher specimens were conserved under the registration numbers (Table 1). The dried and powdered material (100 g) of each plant was macerated in 300 mL of methanol at room temperature for 48 h and then filtered using Whatman filter paper No. 1. The filtrate obtained was concentrated using a rotary evaporator under reduced pressure to obtain the crude methanol extract, which was kept at 4°C until further use.Table 1
Information on the studied plants.
Species (family); voucher number
Traditional uses
Bioactive or potentially bioactive components
Known antimicrobial activities of plants
Azadirachta indica A. Juss (Meliaceae); 4447/SRFK
Antimalarial, anticancer, anti-inflammatory, antidiabetic, antihyperglycaemic, antiulcerous [16]
Alkaloids, glycosides, flavonoids, and saponins [17]
Antibacterial activity of ethanol leaf extract:Ec, Kp, Pm, Sa, Pa, Ef [18]; ethanol and methanol extract of leaves: Ec, Pa, St, Sa, Bp [17]
Citrus grandis (L.) Osbeck (Rutaceae); 25,860/SRFC
Cancer [19, 20]
Alkaloids, cardioglycosides, saponins, tannins, terpenoids, flavonoids, and steroids [21]
Antibacterial activity of petroleum ether, ethyl acetate, chloroform, ethanol, and leaf water:Ec, Sa, Pa, Pm [21]
Cucurbita maxima Duch. (Cucurbitaceae); 42,449/HNC
Diabetes, cancer, antihypertensive, anti-inflammatory, immunomodulating, and bacterial infections [22, 23]
Tannins, saponins, alkaloids [24], cucurbitaxanthin, gibberellin, and α-tocopherol [25]
Antibacterial activity of aqueous seed extract:Ec, Sa, Kp, Ef, Pa [24], ethanol and aqueous extract of flowers: St, Ec, Ef, Bc [26]
Dacryodes edulis (G. Don) H. J. Lam (Burseraceae); 1874/SRFK
Gastrointestinal disorders, toothache, earache [27], dysentery, anemia, leprosy, [28], skin diseases, and sickle cell disease [29]
Ethyl gallate and quercitrin [27]
Antibacterial activity of hydromethanolic, butanol, aqueous, ethanolic extract, ethyl acetate of bark:Ec, Pa, Bc, Sa [27]
Hibiscus esculentus L. (Malvaceae); 42,823/HNC
Inflammation, pain [30], cancer, hypoglycemic [31]
Not reported
Not reported
Ipomoea batatas (L.) Lam (Convolvulaceae); 55,594/HNC
Antidiabetic, anti-inflammatory, reducing risk of cardiovascular disease, anticancer, reducing stomach stress, nausea, diarrhea [32]
Caffeoylquinic acid [33], vitamin E, beta-carotene, lutein, saponins [32], flavonoids, and chitinases [34]
Not reported
Irvingia gabonensis (Aubry. Lec. ex O. Rorke) Baill. (Irvingiaceae); 52,936/HNC
Diarrhea, hernia, yellow fever, dysentery, antipoison [35, 36], gonorrhea, gastrointestinal and hepatic disorders, wound infections, diabetes, analgesic [37]
Saponins, tannins, phenols, and phlobatannins [35], alkaloids, cardiac glycosides, anthraquinones, tannins, flavonoids [36], 3-friedelanone; betulinic acid; oleanolic acid; 3,3,4-tri-O-methylellagic acid; 3,4-di-O-methylellagic acid; hardwickiic acid [38]
Antibacterial activity of aqueous and ethanol extract of leaves and bark:Sa, Ec [36]; S: Bst, Ca, Cf, Ea, Ecl, Mm, Ng, Pa, Pm, Pv, Sa, Sd; Bc, Bm, Bs, Ck, Ec, Kp, Sfl, St, Sf [38]
Phaseolus vulgaris L. (Fabaceae); 42,587/HNC
Antioxidant [39], cancer, estrogenic, antidepressant [40], bacterial infections, tuberculosis [41]
Alkaloids, steroids, and flavonoids [42]
Antibacterial activity of aqueous, alcohol, chloroform, ether extract of seeds:Sa, Pa, Sf, Bs, Kp, Ec [41]
Ricinodendron heudelotii (Baill.) Pierre ex Heckel (Euphorbiaceae); 50,852/HNC
Cough, antidote, intestinal diseases [43], yellow fever, malaria, stomach pain, headache, dysentery [44]
Tannins, polyphenols, alkaloids, glycosides, flavonoids, steroids, and saponins [43], aleuritolic acid, labda-8(17), 13-diem-3β, 15-diol, E-ferulic acid octacosylate [44]
Antibacterial activity of methanol extract of the leaves:Sa, Sf, Pv, Cf, Mm, Ko, Kp, Ec, Pa, St [43], Pf, Bs, Sa, Ec, Ca, Af [44], Ec, Ea, Pa, Ps, Kp, Ecl [18]
Saccharum officinarum L. (Poaceae); 42,958/HNC
Jaundice and liver problems, hemorrhoids, dysentery, menorrhagia [45]
Flavonoids, saponins, tannins, and alkaloids [46]
Antibacterial activity of methanol extract of the stems:Ec, Kp, Sa, Pa [46]
Spondias mombin L. (Anacardiaceae); 21,249/SRFK
Diuretic, febrifuge, diarrheal diseases, dysentery, hemorrhoids, gonorrhea, leucorrhea [47]
Saponins, tannins, flavonoids, alkaloids, and glycosides [48]
Antibacterial activity of ethanol, methanol, water, and acetone extracts of the leaves:Kp, Sa, St, Ea, Sm [48]
Theobroma cacao L. (Sterculiaceae); 60,111/HNC
Cardiovascular, gastrointestinal, and nervous diseases [49]
Alkaloids, anthraquinones, cardiac glycosides, and saponins [50]
Antibacterial activity:Sa, Ec, Sd, Kp, Sm, Pa, Pm [51]
Uapaca guineensis Muell. Arg. (Euphorbiaceae); 53,136/HNC
Leprosy, epilepsy, edema, rheumatism, aphrodisiac, fever, inflammation, absorption [52]
Steroids, alkaloid, terpenoids, and gallic acid [53]
Not reported
HNC: Herbier National du Cameroun; SRFC: Société des Réserves Forestières du Cameroun; SRFK: Société des Réserves forestières du Kamerun; Af:Aspergillus flavus; Bp: Bacillus pumilus; Bc: Bacillus cereus; Bm: Bacillus megaterium; Bs: Bacillus subtilis; Ca: Candida albicans; Cf; Citrobacter freundii; Ck: Candida krusei; Ea: Enterobacter aerogenes; Ec: Escherichia coli; Ecl: Enterobacter cloacae; Ef: Enterococcus faecium; Kp: Klebsiella pneumoniae; Ko: Klebsiella oxytoca; Mm: Morganella morganii; Ng: Neisseria gonorrhoeae; Pa: Pseudomonas aeruginosa; Pf: Pseudomonas fluorescens; Pm: Proteus mirabilis; Ps: Providencia stuartii; Pv: Proteus vulgaris; Sa: Staphylococcus aureus; Sd: Shigella dysenteriae; Sf; Streptococcus faecalis; Sf l: Shigella flexneri; Sm: Serratia marcescens; St: Salmonella typhi.


## 2.2. Chemicals

Eight reference antibiotics (RA) purchased from Sigma-Aldrich (St Quentin Fallavier, France) were tested: ampicillin (AMP), cefepime (CEF), chloramphenicol (CHL), ciprofloxacin (CIP), erythromycin (ERY), kanamycin (KAN), streptomycin (STR), and tetracycline (TET);p-iodonitrotetrazolium chloride (INT) (Sigma-Aldrich) was used as bacterial growth revelator; carbonyl cyanide m-chlorophenyl hydrazone (CCCP) and chlorpromazine (CPZ) (Sigma-Aldrich) were used as efflux pump inhibitors (EPIs); and dimethylsulfoxide (DMSO) was used to dissolve the plant extracts.


## 2.3. Bacteria, Culture Media, and Growth Conditions

The strains ofStaphylococcus aureus used included a reference strain obtained from American Type Culture Collection (ATCC; ATCC 25923), 8 methicillin-resistant S. aureus (MRSA) strains (MSSA1, MRSA3, MRSA4, MRSA6, MRSA8, MRSA9, MRSA11, and MRSA12) (obtained from the culture collection of the Laboratory of Microbiology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Japan, and provided by Dr Jean P. Dzoyem, University of Dschang) [8, 9], and 17 resistant clinical laboratory strains of S. aureus (SA01, SA07, SA18, SA23, SA36, SA39, SA56, SA64, SA68, SA88, SA114, SA116, SA124, SA126, SA127, SA135, and SA139) available in our laboratory collection and previously isolated from patients in Ad-Lucem Hospital in Banka-Bafang (West Region of Cameroon) [54]. Their bacterial features are summarized in Table 2. They were cultured at 37°C overnight on Mueller Hinton agar 24 h prior to any assay. The Mueller Hinton broth (MHB) was used as liquid culture medium for susceptibility tests.Table 2
Bacterial strains and features.
Bacteria
Features
References
ATCC 25923
Reference strain
—
S. aureus MSSA1
Clinical isolate: Met susceptible; Nisr, Chlr
[8, 9]
S. aureus MRSA3
Clinical isolate: Ofxar, Kanr, Tetr, Ermr
[8]
S. aureus MRSA4
Clinical isolate: Ofxar, Kanr, Cypr, Chlr, Genr, Nisr, Ampr
[8, 9]
S. aureus MRSA6
Clinical isolate: Ofxar, Flxr, Kanr, Tetr, Cypr, IM/Csr, Chlr, Genr, Nisr, Ampr
[8, 9]
S. aureus MRSA8
Clinical isolate: Ofxar, Flxr, Kanr, Ermr, Cypr, Im/Csr, Chlr, Genr, Nisr, Ampr
[8, 9]
S. aureus MRSA9
Clinical isolate: Ofxar, Flxr, Tetr, Ermr, Cypr, Im/Csr, Chlr, Genr, Nisr, Ampr
[8, 9]
S. aureus MRSA11
Clinical isolate: Ofxar, Kanr, Ermr, Cypr, Im/Csr, Chlr, Nisr, Ampr
[8, 9]
S. aureus MRSA12
Clinical isolate: Ofxar, Flxr, Kanr, Ermr, Im/Csr, Chlr, Genr, Nisr, Ampr
[8, 9]
SA01
Clinical isolate: Ermr, Ampr
[54]
SA07
Clinical isolate: Ermr, Doxr
[54]
SA18
Clinical isolate: Ampr, Doxr, Vmr
[54]
SA23
Clinical isolate: Imir, Augr
[54]
SA36
Clinical isolate: Doxr, Vmr
[54]
SA39
Clinical isolate: Ampr
[54]
SA56
Clinical isolate: Ampr, Doxr
[54]
SA64
Clinical isolate: Ampr, Doxr
[54]
SA68
Clinical isolate: Ampr, Vmr
[54]
SA88
Clinical isolate: Ermr, Vmr
[54]
SA114
Clinical isolate: Ampr, Doxr
[54]
SA116
Clinical isolate: Ermr
[54]
SA124
Clinical isolate: Ermr
[54]
SA126
Clinical isolate: Ampr, Doxr
[54]
SA127
Clinical isolate: Ampr, Doxr
[54]
SA135
Clinical isolate: Ermr
[54]
SA139
Clinical isolate: Ermr
[54]
Chlr, Cypr, Ermr, Flxr, Im/Csr, Kanr, Metr, Ofxar, Tetr, Vmr, Ampr, Doxr, Augr, Genr, and Nisr resistance to chloramphenicol, ciprofloxacin, erythromycin, flomoxef, imipenem/cilastatin sodium, kanamycin, methicillin, ofloxacin, tetracycline, vancomycin, ampicillin, doxycycline, augmentin, gentamicin, and nisin, respectively, SA: Staphylococcus aureus.


## 2.4. Preliminary Phytochemical Screenings

Potential classes of potential antibacterial phytochemicals such as alkaloids (Dragendorff’s and Mayer’s tests: 5 mg plant extract in 10 mL methanol; a portion of 2 mL extract + 1% HCl + steam, 1 mL filtrate + 6 drops of Mayor’s reagents/Wagner’s reagent/Dragendorff’s reagent; cream precipitate/brownish-red precipitate/orange precipitate indicated the presence of respective alkaloids), saponins (frothing test: 0.5 mL filtrate+ 5 mL distilled water; frothing persistence indicated the presence of saponins), steroids and triterpenoids (Liebermann–Burchard test: 5 mg plant extract in 10 mL chloroform, filtered; a 2 mL filtrate + 2 mL acetic anhydride+ conc. H2SO4; blue-green ring or pink-purple indicated the presence of steroids or triterpenoids), phenolics: anthraquinones (5 mg plant extract in 10 mL methanol; a portion of 2 mL + 2 mL ether-chloroform 1 : 1 v/v + 4 mL NaOH 10% (w/v); red color indicated the presence of anthraquinones), flavonoids (5 mg plant extract in 10 mL methanol; a portion of 2 mL + conc. HCl + magnesium; ribbon pink-tomato red color indicated the presence of flavonoids), polyphenols (ferric chloride test: 5 mg plant material in 10 mL methanol; a portion of 2 mL + 2 mL FeCl3; violet-blue or greenish color indicated the presence of phenols), and tannins (5 mg plant extract in 10 mL distilled water; a portion of 2 mL + 2 mL FeCl3; blue-black precipitate indicated the presence of tannins) (Table 3) were investigated according to the described phytochemical methods [11, 55].Table 3
Extraction yields and phytochemical composition of the plant extracts.
Plant extract and part used
Yields (%)
Alkaloids
Polyphenols
Flavonoids
Anthraquinones
Tannins
Triterpenes
Steroids
Saponins
Azadirachta indica
Bark
10.3
+
+
−
−
+
−
+
+
Citrus grandis
Leaves
2.6
+
+
−
−
+
+
+
−
Cucurbita maxima
Beans
2.6
−
+
−
−
+
+
+
+
Dacryodes edulis
Leaves
6.2
−
+
+
+
+
+
+
+
Bark
9.1
−
+
−
+
+
+
+
+
Seeds
6.9
−
+
+
+
+
+
+
+
Hibiscus esculentus
Leaves
1.9
−
+
−
−
+
−
+
−
Ipomoea batatas
Beans
3.3
+
+
+
+
+
+
−
+
Irvingia gabonensis
Leaves
6.7
−
+
−
−
+
−
+
+
Phaseolus vulgaris
Leaves
1.2
−
+
−
−
+
−
+
+
Ricinodendron heudelotii
Bark
2.9
−
+
+
+
+
+
+
−
Leaves
7.2
−
+
+
+
+
+
+
+
Spondias mombin
Leaves
21.4
−
+
−
−
+
+
+
−
Saccharum officinarum
Leaves
8.4
−
+
−
−
+
−
+
+
Theobroma cacao
Leaves
3.1
−
+
−
−
+
+
+
+
Beans
6.2
+
+
+
+
+
+
+
+
Uapaca guineensis
Leaves
7.3
−
+
−
−
+
+
+
+
Bark
6.1
+
+
−
−
+
+
+
+
−: absent; +: present; yield calculated as the ratio of the mass of the obtained methanol extract/mass of the plant powder.


## 2.5. INT Colorimetric Assay for MIC and Minimal Bactericidal Concentration (MBC) Determinations

The MIC and minimal bactericidal concentration (MBC) determinations on various strains ofS. aureus were performed using the rapid INT colorimetric assay [56] with some modifications as previously described [14, 38]. The samples were dissolved in DMSO/MHB. The final concentration of DMSO was lower than 2.5%. The twofold dilutions of the samples were made in a 96-well microplate, and the tested bacterial concentration was 1.5 × 106 colony-forming unit (CFU)/mL. The microplates were incubated at 37°C for 18 h. All assays were performed in triplicate and repeated thrice. Wells containing MHB, 100 µL of inoculum, and DMSO to a final concentration of 2.5% served as negative control. The MIC of each sample was detected after 18 h incubation at 37°C, following addition (40 µL) of 0.2 mg/mL of INT and incubation at 37°C for 30 minutes as the lowest sample concentration that prevented the color change of the medium and exhibited complete inhibition of microbial growth [56]. The MBC was determined by adding 50 µL aliquots of the preparations, which did not show any growth after incubation during MIC assays, to 150 µL of MHB. These preparations were further incubated at 37°C for 48 h. The MBC was regarded as the lowest concentration of samples, which did not produce a color change after addition of INT as mentioned above [57, 58].


## 2.6. Evaluation of the Role of Efflux Pumps in the Resistance of Selected Bacteria

To evaluate the involvement of efflux pumps in the resistance of selected bacterial strains to some of the active plant extracts,Dacryodes edulis seeds (DES), Hibiscus esculentus leaves (HEL), Uapaca guineensis leaves (UGL), Uapaca guineensis bark (UGB), and CIP (reference drug) were tested in the absence or presence of EPI (CCCP (0.5 µg/mL) or CPZ (25 µg/mL)). MICs of samples alone or in combination with EPI were determined as above, and the increase in activity was determined as the ratio of MIC of sample alone versus sample in combination with EPI. All assays were performed in triplicate and repeated thrice.


## 2.7. Antibiotic Activity Modulation Assays

To evaluate the antibiotic resistance modulation activity of the most active extracts:Azadirachta indica bark (AIB), Dacryodes edulis seeds (DES), Dacryodes edulis bark (DEB), Dacryodes edulis leaves (DEL), Phaseolus vulgaris leaves (PVL), Ricinodendron heudelotii leaves (RHL), and Uapaca guineensis bark (UGB), a preliminary assay was performed in order to assess the MICs of antibiotics in the absence and presence of these extracts using the broth microdilution method as previously described [14, 38, 56]. S. aureus SA88 was used for the preliminary assay, and the samples were tested at various subinhibitory concentrations (MIC/2, MIC/4, MIC/8, and MIC/16). Results allowed to select DEB, DEL, DES, RHL, and UGB to be tested further against S. aureus ATCC 25923, and 8 resistant strains of S. aureus (MRSA3, MRSA4, MRSA9, MRSA11, MRSA12, SA18, SA36, and SA64) at MIC/2 and MIC/4. Briefly, after serial dilutions of antibiotics, extract was added to each well at its subinhibitory concentrations, the bacterial inoculation was done, and the MIC was determined. Rows receiving antibiotic dilutions without extracts were used for the determination of the MICs of the antibiotics. The modulation factor was defined as the ratio of the MIC of antibiotic alone versus that of antibiotic in the presence of extract. Modulation factor ≥2 was set as the cutoff for biologically significance of antibiotic resistance-modulating effects [59].


## 3. Results

### 3.1. Phytochemical Composition of Plant Extracts

The major classes of phytochemicals were screened in the 18 studied plant extracts (Table3). It appears that all extracts contained polyphenols and tannins. Other classes of phytochemicals were selectively present. Only the extract of the beans of Theobroma cacao contained all the investigated classes of secondary metabolites.


### 3.2. Antibacterial Activity

The antibacterial activities of the 18 tested extracts and ciprofloxacin against 26 strains ofS. aureus are summarized in Table 4. It appears that extracts from Dacryodes edulis seeds (DES) and Dacryodes edulis bark (DEB), within a MIC range of 256–1024 µg/mL, and ciprofloxacin (MIC below 4 µg/mL), were active against all 26 tested bacterial strains. Other extracts were selectively active, and MIC values varied from 64 to 1024 µg/mL against 25/26 (96.2%) tested bacteria for Phaseolus vulgaris leaves (PVL), 24/26 (92.3%) for Azadirachta indica bark (AIB), Dacryodes edulis leaves (DEL), and Ricinodendron heudelotii leaves (RHL), 23/26 (88.5%) for Hibiscus esculentus leaves (HEL), 19/26 (73.1%) for Uapaca guineensis leaves (UGL), 18/26 (69.2%) for Ricinodendron heudelotii bark (RHB) and Uapaca guineensis bark (UGB), 17/26 (61.5%) for Saccharum officinarum leaves (SOL), 16/26 (61.5%) for Ipomoea batatas leaves (IBL) and Theobroma cacao leaves (TCL), 15/26 (57.7%) for Citrus grandis leaves (CGL), 14/26 (53.8%) for Theobroma cacao beans (TCB), 12/26 (46.2%) for Cucurbita maxima beans (CMB), 10/26 (38.5%) for Spondias mombin leaves (SML), and 7/26 (26.9%) for Irvingia gabonensis beans (IGB). The lowest MIC value of 64 µg/mL was obtained with CMB against MRSA4 strain and UGB against MRSA9 strain.Table 4
MIC and MBC of the plant extracts and ciprofloxacin againstStaphylococcus aureus strains.
Bacterial strainsa
Samplesb, MIC and MBC in μg/mL (in parentheses)
Plant extracts
Antibiotic
AIB
CGL
CMB
DES
DEB
DEL
HEL
IBL
IGB
PVL
RHB
RHL
SML
SOL
TCB
TCL
UGL
UGB
CIP
ATCC 25923
1024 (–)
–
–
1024 (–)
512 (–)
1024 (–)
–
–
–
512 (–)
1024 (–)
512 (–)
–
1024 (–)
–
–
–
–
<0.5 (16)
SA01
512 (–)
1024 (–)
–
512 (–)
512 (–)
512 (–)
512 (–)
–
–
512 (–)
–
512 (–)
–
1024 (–)
512 (–)
–
512 (–)
512 (–)
<0.5 (4)
SA07
1024 (–)
–
–
512 (–)
512 (–)
512 (–)
512 (–)
1024 (–)
–
1024 (–)
–
512 (–)
–
1024 (–)
–
–
512 (–)
–
<0.5 (1)
SA18
512 (1024)
1024 (–)
–
512 (–)
512 (–)
512 (–)
512 (–)
–
1024 (–)
512 (–)
1024 (–)
512 (–)
–
512 (–)
512 (–)
–
512 (–)
1024 (–)
<0.5 (8)
SA23
1024 (–)
512 (–)
–
512 (–)
512 (–)
1024 (–)
512 (–)
1024 (–)
1024 (–)
512 (–)
1024 (–)
512 (–)
1024 (–)
1024 (–)
512 (–)
512 (–)
512 (–)
512 (–)
<0.5 (<0.5)
SA36
512 (–)
–
1024 (–)
256 (1024)
1024 (–)
1024 (–)
512 (–)
1024 (–)
–
1024 (–)
512 (–)
512 (–)
1024 (–)
1024 (–)
1024 (–)
–
512 (–)
512 (–)
1 (8)
SA39
1024 (–)
1024 (–)
–
512 (–)
1024 (–)
1024 (–)
1024 (–)
–
–
1024 (–)
1024 (–)
–
1024 (–)
–
–
512 (–)
512 (–)
512 (–)
<0.5 (16)
SA56
1024 (–)
–
–
1024 (–)
1024 (–)
1024 (–)
1024 (–)
–
1024 (–)
512 (–)
–
512 (–)
–
1024 (–)
–
–
–
1024 (–)
<0.5 (4)
SA64
512 (–)
512 (–)
1024 (–)
512 (–)
1024 (–)
512 (–)
512 (–)
1024 (–)
–
512 (–)
256 (–)
1024 (–)
1024 (–)
–
–
1024 (–)
512 (–)
1024 (–)
4 (8)
SA68
1024 (–)
–
–
1024 (–)
1024 (–)
1024 (–)
1024 (–)
1024 (–)
–
1024 (–)
1024 (–)
512 (–)
–
–
1024 (–)
–
–
–
<0.5 (<0.5)
SA88
512 (–)
1024 (–)
1024 (–)
512 (–)
1024 (–)
1024 (–)
512 (–)
1024 (–)
–
512 (1024)
–
512 (–)
–
1024 (–)
1024 (–)
1024 (–)
1024 (–)
1024 (–)
<0.5 (2)
SA114
1024 (–)
–
–
1024 (–)
1024 (–)
1024 (–)
256 (–)
512 (–)
–
512 (–)
512 (–)
–
1024 (–)
1024 (–)
–
512 (–)
512 (–)
512 (–)
<0.5 (<0.5)
SA116
–
1024 (–)
–
512 (–)
1024 (–)
512 (–)
512 (–)
–
1024 (–)
1024 (–)
–
1024 (–)
1024 (–)
1024 (–)
–
–
512 (–)
–
<0.5 (<0.5)
SA124
–
1024 (–)
–
512 (–)
1024 (–)
–
–
1024 (–)
–
–
–
1024 (–)
–
–
–
512 (–)
–
–
<0.5 (<0.5)
SA126
1024 (–)
–
1024 (–)
512 (–)
512 (–)
1024 (–)
512 (–)
1024 (–)
–
1024 (–)
1024 (–)
1024 (–)
–
–
–
1024 (–)
1024 (–)
–
<0.5 (<0.5)
SA127
512 (–)
–
–
1024 (–)
1024 (–)
1024 (–)
512 (–)
1024 (–)
–
512 (1024)
–
1024 (–)
1024 (–)
–
–
–
–
–
<0.5 (<0.5)
SA135
1024 (–)
–
–
512 (1024)
1024 (–)
1024 (–)
1024 (–)
–
–
1024 (–)
–
512 (–)
–
1024 (–)
–
–
–
–
<0.5 (1)
SA139
512 (–)
–
–
512 (–)
512 (–)
–
1024 (–)
1024 (–)
–
512 (–)
1024 (–)
512 (–)
–
1024 (–)
–
1024 (–)
–
1024 (–)
<0.5 (<0.5)
MSSA1
512 (–)
–
256 (512)
512 (1024)
1024 (–)
256 (–)
512 (1024)
1024 (–)
–
512 (–)
256 (1024)
512 (1024)
–
1024 (–)
256 (–)
512 (–)
256 (1024)
128 (1024)
2 (16)
MRSA3
512 (1024)
1024 (–)
256 (–)
512 (1024)
1024 (–)
512 (–)
1024 (–)
–
1024 (–)
512 (–)
256 (–)
1024 (–)
512 (–)
–
256 (–)
256 (–)
256 (–)
128 (–)
2 (16)
MRSA4
512 (–)
512 (–)
64 (512)
512 (512)
512 (1024)
256 (–)
1024 (–)
1024 (–)
–
128 (–)
128 (–)
1024 (–)
1024 (–)
1024 (–)
256 (–)
512 (–)
256 (–)
256 (–)
1 (16)
MRSA6
512 (–)
512 (–)
128
512 (–)
1024 (–)
1024 (–)
–
512 (–)
–
1024 (–)
256 (–)
256 (–)
–
–
128 (–)
256 (–)
256 (–)
128 (–)
2 (8)
MRSA8
512 (–)
1024 (–)
128 (1024)
256 (–)
256 (–)
512 (–)
512 (1024)
–
–
512 (–)
256 (–)
512 (–)
–
512 (–)
128 (–)
512 (–)
256 (–)
128 (–)
2 (8)
MRSA9
512 (–)
1024 (–)
128
512 (–)
512 (–)
1024 (–)
512 (–)
256 (–)
1024 (–)
512 (–)
256 (–)
512 (–)
256 (–)
512 (–)
128 (–)
512 (–)
128 (–)
64 (–)
2 (16)
MRSA11
1024 (–)
1024 (–)
256
512 (–)
512 (–)
1024 (–)
512 (–)
1024 (–)
1024 (–)
512 (–)
256 (–)
512 (–)
–
–
128 (–)
512 (–)
256 (–)
128 (–)
2 (16)
MRSA12
512 (–)
1024 (–)
128
512 (–)
256 (–)
256 (–)
512 (–)
–
–
1024 (–)
256 (–)
512 (–)
–
1024 (–)
128 (–)
512 (–)
256 (–)
128 (–)
2 (4)
aBacterial strain (SA: Staphylococcus aureus; MRSA: methicillin-resistant Staphylococcus aureus), bsamples (AIB: Azadirachta indica bark, CGL: Citrus grandis, CMB: Cucurbita maxima beans, DES: Dacryodes edulis seeds, DEB: Dacryodes edulis bark, DEL: Dacryodes edulis leaves, HEL: Hibiscus esculentus leaves, IBL: Ipomoea batatas leaves, IGB: Irvingia gabonensis beans, PVL: Phaseolus vulgaris leaves, RHL: Ricinodendron heudelotii leaves, RHB: Ricinodendron heudelotii bark, SML: Spondias mombin leaves, SOL: Saccharum officinarum leaves, TCB: Theobroma cacao beans, TCL: Theobroma cacao leaves, UGL: Uapaca guineensis leaves, UGB: Uapaca guineensis bark, and CIP: ciprofloxacin); R: MBC/MIC; (–): >1024(MIC); nc, not calculated; MIC: minimal inhibitory concentration; MBC: minimal bactericidal concentration; CIP: ciprofloxacin; –: MIC and MBC at up to 1024 μg/mL; MIC in bold: significant activity [37, 60].


### 3.3. Role of Efflux Pumps in the Resistance of Strains ofS. aureus

Four extracts (DES, HEL, UGL, and UGB) and CIP (reference drug) were tested in the absence or presence of CCCP (0.5µg/mL) and CPZ (25 µg/mL) to evaluate the role of efflux pumps in the resistance of 14 tested S. aureus strains. The results are summarized in Tables 5 and 6. It appears that CCCP significantly improved the activity of the 4 tested extracts against the majority of S. aureus strains (Table 5). The increase of activity in the presence of CCCP ranged from 2-fold to >128-fold. The highest increase of activity (>128-fold) was obtained when DES, UGB, and UGL were tested in the presence of CCCP on at least one S. aureus strain. In contrast, in the presence of CPZ, no improvement in the activity of the four extracts was observed (Table 6). This is clear indication that CCCP was the appropriate EPI of the studied S. aureus strains.Table 5
MIC of extracts and ciprofloxacin in the absence (−) and presence (+) of carbonyl cyanidem-chlorophenyl hydrazone (CCCP) against selected strains of Staphylococcus aureus.
Bacterial strainsa
Samplesb and MIC in µg/mL and fold increase of activity (in parentheses)
DES
HEL
UGB
UGL
CIP
−
+
−
+
−
+
−
+
−
+
ATCC 25923
256
32(32)
256
<8(>32)
–
<8(>128)
1024
<8(>128)
2
1(2)
MRSA3
256
<8(>32)
256
<8(>32)
512
<8(>64)
512
<8(>64)
1
<0.5(>2)
MRSA4
256
16(16)
128
16(8)
1024
128(8)
1024
64(16)
2
<0.5(>4)
MRSA6
128
<8(>16)
256
<8(>32)
1024
<8(>128)
512
<8(>64)
1
<0.5(>2)
MRSA8
256
128(2)
128
128 (1)
–
512(>2)
512
128(>4)
<0.5
<0.5 (nd)
MRSA9
128
<8(>16)
64
<8(>8)
1024
<8(>128)
256
<8(>32)
<0.5
<0.5 (nd)
MRSA11
256
<8(>32)
128
<8(>16)
1024
<8(>128)
256
<8(>32)
1
1(1)
MRSA12
256
<8(>32)
128
<8(>16)
1024
<8(>128)
256
<8(>32)
<0.5
<0.5 (nd)
SA01
128
<8(>16)
128
<8(>16)
1024
<8(>128)
256
<8(>32)
<0.5
<0.5 (nd)
SA07
512
<8(>64)
512
512 (1)
512
512 (1)
256
<8(>32)
<0.5
<0.5 (nd)
SA18
1024
128(8)
256
128(2)
1024
512(2)
512
<8(>64)
1
<0.5(>2)
SA88
512
512 (1)
–
–
1024
1024 (1)
1024
256(>4)
1
<0.5(>2)
SA114
1024
<8(>128)
–
–
1024
128(8)
512
<8(>64)
<0.5
<0.5(>2)
SA135
512
64(8)
–
–
–
128(>8)
512
<8(>64)
1
<0.5(>2)
aBacterial strain (SA: Staphylococcus aureus; MRSA: methicillin-resistant Staphylococcus aureus), bsamples (DES: Dacryodes edulis seeds, HEL: Hibiscus esculentus leaves, UGL: Uapaca guineensis leaves, UGB: Uapaca guineensis bark, and CIP: ciprofloxacin); MIC: minimal inhibitory concentration; CCCP was tested at 0.5 µg/mL; (–): >1024 µg/mL; values in bold represent increase of activity ≥2.Table 6
MIC of extracts and ciprofloxacin in the absence (−) and presence (+) of chlorpromazine (CPZ) against selected strains ofStaphylococcus aureus.
Bacterial strainsa
Samplesb and MIC in µg/mL and fold increase of activity (in parentheses)
DES
HEL
UGB
UGL
CIP
−
+
−
+
−
+
−
+
−
+
ATCC 25923
512
512 (1)
−
−
512
512 (1)
128
1024 (0.13)
2
2 (1)
MRSA3
256
256 (1)
1024
1024 (1)
512
512 (1)
−
−
4
4 (1)
MRSA4
512
512 (1)
−
−
256
256 (1)
1024
1024 (1)
2
2 (1)
MRSA6
256
256 (1)
512
128 (2)
512
512 (1)
256
−(0.25)
2
2 (1)
MRSA8
256
256 (1)
1024
1024 (1)
256
256 (1)
−
−
1
2 (0.5)
MRSA9
256
256 (1)
1024
1024 (1)
512
512 (1)
256
−(0.25)
1
2 (0.5)
MRSA11
512
512 (1)
1024
1024 (1)
512
512 (1)
128
−(<0.13)
4
4 (1)
MRSA12
512
512 (1)
−
−
512
512 (1)
−
−
2
2 (1)
SA01
512
512 (1)
1024
1024 (1)
512
512 (1)
512
512 (1)
1
1 (1)
SA07
512
512 (1)
1024
1024 (1)
512
512 (1)
1024
1024 (1)
4
4 (1)
SA18
512
512 (1)
−
−
1024
1024 (1)
−
−
1
1 (1)
SA88
512
512 (1)
−
−
1024
1024 (1)
−
−
4
4 (1)
SA114
1024
1024 (1)
−
−
1024
1024 (1)
−
−
1
1 (1)
SA135
512
512 (1)
−
−
1024
1024 (1)
−
−
1
1 (1)
aBacterial strain (SA: Staphylococcus aureus; MRSA: methicillin-resistant Staphylococcus aureus); bsamples (DES: Dacryodes edulis seeds, HEL: Hibiscus esculentus leaves, UGL: Uapaca guineensis leaves, UGB: Uapaca guineensis bark, CIP: ciprofloxacin); CPZ: chlorpromazine at 25 µg/mL; CIP: ciprofloxacin; MIC: minimal inhibitory concentration.


### 3.4. Antibiotic Resistance Modulation Activity of Extracts

Seven plant extracts, AIB, DES, DEB, DEL, PVL, RHL, and UGB, at their various subinhibitory concentrations (MIC/2, MIC/4, MIC/8, and MIC/16) were first tested in combination with 8 antibiotics: CHL, TET, CIP, AMP, CEF, ERY, STR, and KAN againstS. aureus SA88 strain. The results summarized in Table S1 (Supplementary Materials) show that better modulation of the activity of antibiotics was obtained with all extracts at MIC/2 and MIC/4. At their MIC/2, 2-fold or more increase of antibiotic activities was obtained with PVL, AIB, DEB, DES, DEL, UGB, and RHL and 2, 4, 4, 6, 6, 7, and 8 of the 8 tested antibiotics, respectively (Table S1; Supplementary Materials). Consequently, the most active extracts, DEB, DEL, DES, RHL, and UGB, were further tested in combination with the above 8 antibiotics against the reference strains (ATCC 25923) and 8 resistant strains of S. aureus at MIC/2 and MIC/4 (Tables S2–S6; Supplementary Materials). Results showed that 2-fold or more antibiotic-modulating effects against more than 70% of the S. aureus strains tested were obtained when DEB was combined with CHL at MIC/2 (77.78%; Table S2; Supplementary Materials), when DEL was combined with CHL and STR at MIC/2 (77.78%; Table S3; Supplementary Materials), when DES was combined with CIP (77.78% at MIC/2), CHL (100% and 88.89 at MIC/2 and MIC/4 resp.), TET (77.78% at MIC/2 and MIC/4), and STR (88.89% and 77.78% at MIC/2 and MIC/4 resp.) (Table S4; Supplementary Materials), when RHL was combined with CIP, ERY, and KAN (88.89% and 77.78% at MIC/2 and MIC/4 resp.), CHL (88.89% at MIC/2 and MIC/4), TET (77.78% at MIC/2 and MIC/4), and STR (88.89% at MIC/2) (Table S5; Supplementary Materials), and when UGB was combined with CHL, KAN, and STR (77.78% at MIC/2 and MIC/4) (Table S6; Supplementary Materials).


## 3.1. Phytochemical Composition of Plant Extracts

The major classes of phytochemicals were screened in the 18 studied plant extracts (Table3). It appears that all extracts contained polyphenols and tannins. Other classes of phytochemicals were selectively present. Only the extract of the beans of Theobroma cacao contained all the investigated classes of secondary metabolites.


## 3.2. Antibacterial Activity

The antibacterial activities of the 18 tested extracts and ciprofloxacin against 26 strains ofS. aureus are summarized in Table 4. It appears that extracts from Dacryodes edulis seeds (DES) and Dacryodes edulis bark (DEB), within a MIC range of 256–1024 µg/mL, and ciprofloxacin (MIC below 4 µg/mL), were active against all 26 tested bacterial strains. Other extracts were selectively active, and MIC values varied from 64 to 1024 µg/mL against 25/26 (96.2%) tested bacteria for Phaseolus vulgaris leaves (PVL), 24/26 (92.3%) for Azadirachta indica bark (AIB), Dacryodes edulis leaves (DEL), and Ricinodendron heudelotii leaves (RHL), 23/26 (88.5%) for Hibiscus esculentus leaves (HEL), 19/26 (73.1%) for Uapaca guineensis leaves (UGL), 18/26 (69.2%) for Ricinodendron heudelotii bark (RHB) and Uapaca guineensis bark (UGB), 17/26 (61.5%) for Saccharum officinarum leaves (SOL), 16/26 (61.5%) for Ipomoea batatas leaves (IBL) and Theobroma cacao leaves (TCL), 15/26 (57.7%) for Citrus grandis leaves (CGL), 14/26 (53.8%) for Theobroma cacao beans (TCB), 12/26 (46.2%) for Cucurbita maxima beans (CMB), 10/26 (38.5%) for Spondias mombin leaves (SML), and 7/26 (26.9%) for Irvingia gabonensis beans (IGB). The lowest MIC value of 64 µg/mL was obtained with CMB against MRSA4 strain and UGB against MRSA9 strain.Table 4
MIC and MBC of the plant extracts and ciprofloxacin againstStaphylococcus aureus strains.
Bacterial strainsa
Samplesb, MIC and MBC in μg/mL (in parentheses)
Plant extracts
Antibiotic
AIB
CGL
CMB
DES
DEB
DEL
HEL
IBL
IGB
PVL
RHB
RHL
SML
SOL
TCB
TCL
UGL
UGB
CIP
ATCC 25923
1024 (–)
–
–
1024 (–)
512 (–)
1024 (–)
–
–
–
512 (–)
1024 (–)
512 (–)
–
1024 (–)
–
–
–
–
<0.5 (16)
SA01
512 (–)
1024 (–)
–
512 (–)
512 (–)
512 (–)
512 (–)
–
–
512 (–)
–
512 (–)
–
1024 (–)
512 (–)
–
512 (–)
512 (–)
<0.5 (4)
SA07
1024 (–)
–
–
512 (–)
512 (–)
512 (–)
512 (–)
1024 (–)
–
1024 (–)
–
512 (–)
–
1024 (–)
–
–
512 (–)
–
<0.5 (1)
SA18
512 (1024)
1024 (–)
–
512 (–)
512 (–)
512 (–)
512 (–)
–
1024 (–)
512 (–)
1024 (–)
512 (–)
–
512 (–)
512 (–)
–
512 (–)
1024 (–)
<0.5 (8)
SA23
1024 (–)
512 (–)
–
512 (–)
512 (–)
1024 (–)
512 (–)
1024 (–)
1024 (–)
512 (–)
1024 (–)
512 (–)
1024 (–)
1024 (–)
512 (–)
512 (–)
512 (–)
512 (–)
<0.5 (<0.5)
SA36
512 (–)
–
1024 (–)
256 (1024)
1024 (–)
1024 (–)
512 (–)
1024 (–)
–
1024 (–)
512 (–)
512 (–)
1024 (–)
1024 (–)
1024 (–)
–
512 (–)
512 (–)
1 (8)
SA39
1024 (–)
1024 (–)
–
512 (–)
1024 (–)
1024 (–)
1024 (–)
–
–
1024 (–)
1024 (–)
–
1024 (–)
–
–
512 (–)
512 (–)
512 (–)
<0.5 (16)
SA56
1024 (–)
–
–
1024 (–)
1024 (–)
1024 (–)
1024 (–)
–
1024 (–)
512 (–)
–
512 (–)
–
1024 (–)
–
–
–
1024 (–)
<0.5 (4)
SA64
512 (–)
512 (–)
1024 (–)
512 (–)
1024 (–)
512 (–)
512 (–)
1024 (–)
–
512 (–)
256 (–)
1024 (–)
1024 (–)
–
–
1024 (–)
512 (–)
1024 (–)
4 (8)
SA68
1024 (–)
–
–
1024 (–)
1024 (–)
1024 (–)
1024 (–)
1024 (–)
–
1024 (–)
1024 (–)
512 (–)
–
–
1024 (–)
–
–
–
<0.5 (<0.5)
SA88
512 (–)
1024 (–)
1024 (–)
512 (–)
1024 (–)
1024 (–)
512 (–)
1024 (–)
–
512 (1024)
–
512 (–)
–
1024 (–)
1024 (–)
1024 (–)
1024 (–)
1024 (–)
<0.5 (2)
SA114
1024 (–)
–
–
1024 (–)
1024 (–)
1024 (–)
256 (–)
512 (–)
–
512 (–)
512 (–)
–
1024 (–)
1024 (–)
–
512 (–)
512 (–)
512 (–)
<0.5 (<0.5)
SA116
–
1024 (–)
–
512 (–)
1024 (–)
512 (–)
512 (–)
–
1024 (–)
1024 (–)
–
1024 (–)
1024 (–)
1024 (–)
–
–
512 (–)
–
<0.5 (<0.5)
SA124
–
1024 (–)
–
512 (–)
1024 (–)
–
–
1024 (–)
–
–
–
1024 (–)
–
–
–
512 (–)
–
–
<0.5 (<0.5)
SA126
1024 (–)
–
1024 (–)
512 (–)
512 (–)
1024 (–)
512 (–)
1024 (–)
–
1024 (–)
1024 (–)
1024 (–)
–
–
–
1024 (–)
1024 (–)
–
<0.5 (<0.5)
SA127
512 (–)
–
–
1024 (–)
1024 (–)
1024 (–)
512 (–)
1024 (–)
–
512 (1024)
–
1024 (–)
1024 (–)
–
–
–
–
–
<0.5 (<0.5)
SA135
1024 (–)
–
–
512 (1024)
1024 (–)
1024 (–)
1024 (–)
–
–
1024 (–)
–
512 (–)
–
1024 (–)
–
–
–
–
<0.5 (1)
SA139
512 (–)
–
–
512 (–)
512 (–)
–
1024 (–)
1024 (–)
–
512 (–)
1024 (–)
512 (–)
–
1024 (–)
–
1024 (–)
–
1024 (–)
<0.5 (<0.5)
MSSA1
512 (–)
–
256 (512)
512 (1024)
1024 (–)
256 (–)
512 (1024)
1024 (–)
–
512 (–)
256 (1024)
512 (1024)
–
1024 (–)
256 (–)
512 (–)
256 (1024)
128 (1024)
2 (16)
MRSA3
512 (1024)
1024 (–)
256 (–)
512 (1024)
1024 (–)
512 (–)
1024 (–)
–
1024 (–)
512 (–)
256 (–)
1024 (–)
512 (–)
–
256 (–)
256 (–)
256 (–)
128 (–)
2 (16)
MRSA4
512 (–)
512 (–)
64 (512)
512 (512)
512 (1024)
256 (–)
1024 (–)
1024 (–)
–
128 (–)
128 (–)
1024 (–)
1024 (–)
1024 (–)
256 (–)
512 (–)
256 (–)
256 (–)
1 (16)
MRSA6
512 (–)
512 (–)
128
512 (–)
1024 (–)
1024 (–)
–
512 (–)
–
1024 (–)
256 (–)
256 (–)
–
–
128 (–)
256 (–)
256 (–)
128 (–)
2 (8)
MRSA8
512 (–)
1024 (–)
128 (1024)
256 (–)
256 (–)
512 (–)
512 (1024)
–
–
512 (–)
256 (–)
512 (–)
–
512 (–)
128 (–)
512 (–)
256 (–)
128 (–)
2 (8)
MRSA9
512 (–)
1024 (–)
128
512 (–)
512 (–)
1024 (–)
512 (–)
256 (–)
1024 (–)
512 (–)
256 (–)
512 (–)
256 (–)
512 (–)
128 (–)
512 (–)
128 (–)
64 (–)
2 (16)
MRSA11
1024 (–)
1024 (–)
256
512 (–)
512 (–)
1024 (–)
512 (–)
1024 (–)
1024 (–)
512 (–)
256 (–)
512 (–)
–
–
128 (–)
512 (–)
256 (–)
128 (–)
2 (16)
MRSA12
512 (–)
1024 (–)
128
512 (–)
256 (–)
256 (–)
512 (–)
–
–
1024 (–)
256 (–)
512 (–)
–
1024 (–)
128 (–)
512 (–)
256 (–)
128 (–)
2 (4)
aBacterial strain (SA: Staphylococcus aureus; MRSA: methicillin-resistant Staphylococcus aureus), bsamples (AIB: Azadirachta indica bark, CGL: Citrus grandis, CMB: Cucurbita maxima beans, DES: Dacryodes edulis seeds, DEB: Dacryodes edulis bark, DEL: Dacryodes edulis leaves, HEL: Hibiscus esculentus leaves, IBL: Ipomoea batatas leaves, IGB: Irvingia gabonensis beans, PVL: Phaseolus vulgaris leaves, RHL: Ricinodendron heudelotii leaves, RHB: Ricinodendron heudelotii bark, SML: Spondias mombin leaves, SOL: Saccharum officinarum leaves, TCB: Theobroma cacao beans, TCL: Theobroma cacao leaves, UGL: Uapaca guineensis leaves, UGB: Uapaca guineensis bark, and CIP: ciprofloxacin); R: MBC/MIC; (–): >1024(MIC); nc, not calculated; MIC: minimal inhibitory concentration; MBC: minimal bactericidal concentration; CIP: ciprofloxacin; –: MIC and MBC at up to 1024 μg/mL; MIC in bold: significant activity [37, 60].


## 3.3. Role of Efflux Pumps in the Resistance of Strains ofS. aureus

Four extracts (DES, HEL, UGL, and UGB) and CIP (reference drug) were tested in the absence or presence of CCCP (0.5µg/mL) and CPZ (25 µg/mL) to evaluate the role of efflux pumps in the resistance of 14 tested S. aureus strains. The results are summarized in Tables 5 and 6. It appears that CCCP significantly improved the activity of the 4 tested extracts against the majority of S. aureus strains (Table 5). The increase of activity in the presence of CCCP ranged from 2-fold to >128-fold. The highest increase of activity (>128-fold) was obtained when DES, UGB, and UGL were tested in the presence of CCCP on at least one S. aureus strain. In contrast, in the presence of CPZ, no improvement in the activity of the four extracts was observed (Table 6). This is clear indication that CCCP was the appropriate EPI of the studied S. aureus strains.Table 5
MIC of extracts and ciprofloxacin in the absence (−) and presence (+) of carbonyl cyanidem-chlorophenyl hydrazone (CCCP) against selected strains of Staphylococcus aureus.
Bacterial strainsa
Samplesb and MIC in µg/mL and fold increase of activity (in parentheses)
DES
HEL
UGB
UGL
CIP
−
+
−
+
−
+
−
+
−
+
ATCC 25923
256
32(32)
256
<8(>32)
–
<8(>128)
1024
<8(>128)
2
1(2)
MRSA3
256
<8(>32)
256
<8(>32)
512
<8(>64)
512
<8(>64)
1
<0.5(>2)
MRSA4
256
16(16)
128
16(8)
1024
128(8)
1024
64(16)
2
<0.5(>4)
MRSA6
128
<8(>16)
256
<8(>32)
1024
<8(>128)
512
<8(>64)
1
<0.5(>2)
MRSA8
256
128(2)
128
128 (1)
–
512(>2)
512
128(>4)
<0.5
<0.5 (nd)
MRSA9
128
<8(>16)
64
<8(>8)
1024
<8(>128)
256
<8(>32)
<0.5
<0.5 (nd)
MRSA11
256
<8(>32)
128
<8(>16)
1024
<8(>128)
256
<8(>32)
1
1(1)
MRSA12
256
<8(>32)
128
<8(>16)
1024
<8(>128)
256
<8(>32)
<0.5
<0.5 (nd)
SA01
128
<8(>16)
128
<8(>16)
1024
<8(>128)
256
<8(>32)
<0.5
<0.5 (nd)
SA07
512
<8(>64)
512
512 (1)
512
512 (1)
256
<8(>32)
<0.5
<0.5 (nd)
SA18
1024
128(8)
256
128(2)
1024
512(2)
512
<8(>64)
1
<0.5(>2)
SA88
512
512 (1)
–
–
1024
1024 (1)
1024
256(>4)
1
<0.5(>2)
SA114
1024
<8(>128)
–
–
1024
128(8)
512
<8(>64)
<0.5
<0.5(>2)
SA135
512
64(8)
–
–
–
128(>8)
512
<8(>64)
1
<0.5(>2)
aBacterial strain (SA: Staphylococcus aureus; MRSA: methicillin-resistant Staphylococcus aureus), bsamples (DES: Dacryodes edulis seeds, HEL: Hibiscus esculentus leaves, UGL: Uapaca guineensis leaves, UGB: Uapaca guineensis bark, and CIP: ciprofloxacin); MIC: minimal inhibitory concentration; CCCP was tested at 0.5 µg/mL; (–): >1024 µg/mL; values in bold represent increase of activity ≥2.Table 6
MIC of extracts and ciprofloxacin in the absence (−) and presence (+) of chlorpromazine (CPZ) against selected strains ofStaphylococcus aureus.
Bacterial strainsa
Samplesb and MIC in µg/mL and fold increase of activity (in parentheses)
DES
HEL
UGB
UGL
CIP
−
+
−
+
−
+
−
+
−
+
ATCC 25923
512
512 (1)
−
−
512
512 (1)
128
1024 (0.13)
2
2 (1)
MRSA3
256
256 (1)
1024
1024 (1)
512
512 (1)
−
−
4
4 (1)
MRSA4
512
512 (1)
−
−
256
256 (1)
1024
1024 (1)
2
2 (1)
MRSA6
256
256 (1)
512
128 (2)
512
512 (1)
256
−(0.25)
2
2 (1)
MRSA8
256
256 (1)
1024
1024 (1)
256
256 (1)
−
−
1
2 (0.5)
MRSA9
256
256 (1)
1024
1024 (1)
512
512 (1)
256
−(0.25)
1
2 (0.5)
MRSA11
512
512 (1)
1024
1024 (1)
512
512 (1)
128
−(<0.13)
4
4 (1)
MRSA12
512
512 (1)
−
−
512
512 (1)
−
−
2
2 (1)
SA01
512
512 (1)
1024
1024 (1)
512
512 (1)
512
512 (1)
1
1 (1)
SA07
512
512 (1)
1024
1024 (1)
512
512 (1)
1024
1024 (1)
4
4 (1)
SA18
512
512 (1)
−
−
1024
1024 (1)
−
−
1
1 (1)
SA88
512
512 (1)
−
−
1024
1024 (1)
−
−
4
4 (1)
SA114
1024
1024 (1)
−
−
1024
1024 (1)
−
−
1
1 (1)
SA135
512
512 (1)
−
−
1024
1024 (1)
−
−
1
1 (1)
aBacterial strain (SA: Staphylococcus aureus; MRSA: methicillin-resistant Staphylococcus aureus); bsamples (DES: Dacryodes edulis seeds, HEL: Hibiscus esculentus leaves, UGL: Uapaca guineensis leaves, UGB: Uapaca guineensis bark, CIP: ciprofloxacin); CPZ: chlorpromazine at 25 µg/mL; CIP: ciprofloxacin; MIC: minimal inhibitory concentration.


## 3.4. Antibiotic Resistance Modulation Activity of Extracts

Seven plant extracts, AIB, DES, DEB, DEL, PVL, RHL, and UGB, at their various subinhibitory concentrations (MIC/2, MIC/4, MIC/8, and MIC/16) were first tested in combination with 8 antibiotics: CHL, TET, CIP, AMP, CEF, ERY, STR, and KAN againstS. aureus SA88 strain. The results summarized in Table S1 (Supplementary Materials) show that better modulation of the activity of antibiotics was obtained with all extracts at MIC/2 and MIC/4. At their MIC/2, 2-fold or more increase of antibiotic activities was obtained with PVL, AIB, DEB, DES, DEL, UGB, and RHL and 2, 4, 4, 6, 6, 7, and 8 of the 8 tested antibiotics, respectively (Table S1; Supplementary Materials). Consequently, the most active extracts, DEB, DEL, DES, RHL, and UGB, were further tested in combination with the above 8 antibiotics against the reference strains (ATCC 25923) and 8 resistant strains of S. aureus at MIC/2 and MIC/4 (Tables S2–S6; Supplementary Materials). Results showed that 2-fold or more antibiotic-modulating effects against more than 70% of the S. aureus strains tested were obtained when DEB was combined with CHL at MIC/2 (77.78%; Table S2; Supplementary Materials), when DEL was combined with CHL and STR at MIC/2 (77.78%; Table S3; Supplementary Materials), when DES was combined with CIP (77.78% at MIC/2), CHL (100% and 88.89 at MIC/2 and MIC/4 resp.), TET (77.78% at MIC/2 and MIC/4), and STR (88.89% and 77.78% at MIC/2 and MIC/4 resp.) (Table S4; Supplementary Materials), when RHL was combined with CIP, ERY, and KAN (88.89% and 77.78% at MIC/2 and MIC/4 resp.), CHL (88.89% at MIC/2 and MIC/4), TET (77.78% at MIC/2 and MIC/4), and STR (88.89% at MIC/2) (Table S5; Supplementary Materials), and when UGB was combined with CHL, KAN, and STR (77.78% at MIC/2 and MIC/4) (Table S6; Supplementary Materials).


## 4. Discussion

### 4.1. Phytochemical Composition of Extracts

Polyphenols and tannins were detected in all extracts. The role of several molecules belonging to polyphenols as antibacterials has been demonstrated [10, 11, 38, 61]. Tannins also belong to a class of polyphenols, and its presence in all extracts could in part explain the fact that all the tested extracts were active in at least one strain of the tested bacteria [10]. However, it should be made clear that the presence of a class of secondary metabolite with reported antibacterial effect is not a guarantee of the good activity of a plant. The antibacterial effect depends on the structure and the amount of a particular phytochemical in the plant or possible interactions with other compounds. This could explain why the extract from the beans of Theobroma cacao that contained all the investigated classes of secondary metabolites (Table 3) was not the most active sample (Table 4).


### 4.2. Antibacterial Potential of Extracts

Resistance of bacteria to antibiotics propels the search of new agents to fight against MDR phenotypes. In the present study, clinical strains ofS. aureus used were previously reported as resistant to at least one commonly used antibiotic [8, 9] (Table 2). Several locally isolated strains of S. aureus [54] were used herein, to better adapt the study to our environment. According to established criteria, MIC values in the range of 100–1000 µg/mL are indication that plant extracts bear antimicrobial activities [62]. Also, the antibacterial activity of botanicals is considered significant if MIC values are below 100 µg/mL, moderate if 100 ≤ MICs ≤ 625 µg/mL, and weak if MICs > 625 µg/mL [37, 60]. On these bases, it can be deduced that all the tested plant extracts had antistaphylococcal activities, except Irvingia gabonensis beans (IGB), with MICs above 1000 µg/mL against all tested strains of S. aureus (Table 4). This activity was significant for CMB against MRSA4 strain and UGB against MRSA9 strain (MIC: 64 µg/mL). Most of the recorded MIC values ranged from 512 to 1024 µg/mL, indicating that extracts rather exhibited moderate to low antistaphylococcal effects. However, this activity could be considered important because the clinical strains of S. aureus used were resistant phenotypes while extracts were from edible plants. In effect, it was suggested that if botanicals are food plants, as they are allegedly nontoxic or less toxic than other medicinal plants, their antibacterial activity is significant in a range of 100 ≤ MIC ≤ 512 µg/mL and moderately active in a range of 512 < MIC ≤ 2048 µg/mL [63].


### 4.3. Role of Efflux Pumps in Susceptibility ofS. aureus Strains to the Extracts

Bacterial efflux systems are associated with major human health concerns as they are involved in the resistance of pathogenic bacteria such asS. aureus [64–66]. Previously, inhibition of efflux pumps by natural products has been found to improve the activity of antibiotics against S. aureus. For example, inhibition of the TetK efflux pump was reported with the essential oil of Chenopodium ambrosioides and its constituent α-terpinene against S. aureus IS-58 strain [66]. In the present study, two well-known EPIs (CCCP and CPZ) were used to assess the implication of efflux pumps in the resistance of the studied S. aureus strains to plant extracts. CCCP is an inhibitor of the proton-motive force of ATP-binding cassette (ABC) transporters of several Gram-negative and Gram-positive bacteria, including S. aureus [5–7]. CPZ is capable of reversing or reducing the antibiotic resistance of bacteria including S. aureus [67], due to its indirect effects on ATPase activity that is dependent upon Ca2+ [68]. In the present study, it was found that CCCP contrary to CPZ improved the activity of the four extracts (DES, HEL, UGL, and UGB) (Tables 5 and 6). This indicates that ABC transporters are involved in the resistance of the studied strains of S. aureus and that combination of extracts such as HEL, UGL, and UGB with an inhibitor of ABC transporters could improve the antistaphylococcal fight.


### 4.4. Antibiotic Modulation Effects of Extracts

The antibiotic resistance-modulating effects of several botanicals and phytochemicals against resistant bacteria have been documented [12–15, 59, 69]. It has been suggested that extracts capable of potentiating the activity of antibiotics on more than 70% of bacteria could be potential efflux pump inhibitors [70]. In this study, antibiotic modulation activity of extracts at their MIC/2 on more than 70% tested strains of S. aureus was obtained with the association of DEB and DEL and with 1/8 (12.5%) tested antibiotics (Tables S2 and S3; Supplementary Materials), UGB with 2/8 (25%) antibiotics (Table S6; Supplementary Materials), DES with 4/8 (50%) antibiotics (Table S4; Supplementary Materials), and RHL with 6/8 (75%) antibiotics (Table S5; Supplementary Materials). Hence, the tested extracts and mostly RHL may act as efflux pump inhibitors [70]. The use of CCCP indicated that ABC transporters were the efflux pumps involved in the resistance of the tested bacteria, suggesting that the above extract could be the inhibitors of such pumps. The potential of the R. heudelotii leaf extract (RHL) to reverse antibiotic resistance in Gram-negative MDR bacteria was previously reported [18]. The present study therefore provides more information about the ability of this plant to modulate the activity of antibiotics against resistant strains of S. aureus.


## 4.1. Phytochemical Composition of Extracts

Polyphenols and tannins were detected in all extracts. The role of several molecules belonging to polyphenols as antibacterials has been demonstrated [10, 11, 38, 61]. Tannins also belong to a class of polyphenols, and its presence in all extracts could in part explain the fact that all the tested extracts were active in at least one strain of the tested bacteria [10]. However, it should be made clear that the presence of a class of secondary metabolite with reported antibacterial effect is not a guarantee of the good activity of a plant. The antibacterial effect depends on the structure and the amount of a particular phytochemical in the plant or possible interactions with other compounds. This could explain why the extract from the beans of Theobroma cacao that contained all the investigated classes of secondary metabolites (Table 3) was not the most active sample (Table 4).


## 4.2. Antibacterial Potential of Extracts

Resistance of bacteria to antibiotics propels the search of new agents to fight against MDR phenotypes. In the present study, clinical strains ofS. aureus used were previously reported as resistant to at least one commonly used antibiotic [8, 9] (Table 2). Several locally isolated strains of S. aureus [54] were used herein, to better adapt the study to our environment. According to established criteria, MIC values in the range of 100–1000 µg/mL are indication that plant extracts bear antimicrobial activities [62]. Also, the antibacterial activity of botanicals is considered significant if MIC values are below 100 µg/mL, moderate if 100 ≤ MICs ≤ 625 µg/mL, and weak if MICs > 625 µg/mL [37, 60]. On these bases, it can be deduced that all the tested plant extracts had antistaphylococcal activities, except Irvingia gabonensis beans (IGB), with MICs above 1000 µg/mL against all tested strains of S. aureus (Table 4). This activity was significant for CMB against MRSA4 strain and UGB against MRSA9 strain (MIC: 64 µg/mL). Most of the recorded MIC values ranged from 512 to 1024 µg/mL, indicating that extracts rather exhibited moderate to low antistaphylococcal effects. However, this activity could be considered important because the clinical strains of S. aureus used were resistant phenotypes while extracts were from edible plants. In effect, it was suggested that if botanicals are food plants, as they are allegedly nontoxic or less toxic than other medicinal plants, their antibacterial activity is significant in a range of 100 ≤ MIC ≤ 512 µg/mL and moderately active in a range of 512 < MIC ≤ 2048 µg/mL [63].


## 4.3. Role of Efflux Pumps in Susceptibility ofS. aureus Strains to the Extracts

Bacterial efflux systems are associated with major human health concerns as they are involved in the resistance of pathogenic bacteria such asS. aureus [64–66]. Previously, inhibition of efflux pumps by natural products has been found to improve the activity of antibiotics against S. aureus. For example, inhibition of the TetK efflux pump was reported with the essential oil of Chenopodium ambrosioides and its constituent α-terpinene against S. aureus IS-58 strain [66]. In the present study, two well-known EPIs (CCCP and CPZ) were used to assess the implication of efflux pumps in the resistance of the studied S. aureus strains to plant extracts. CCCP is an inhibitor of the proton-motive force of ATP-binding cassette (ABC) transporters of several Gram-negative and Gram-positive bacteria, including S. aureus [5–7]. CPZ is capable of reversing or reducing the antibiotic resistance of bacteria including S. aureus [67], due to its indirect effects on ATPase activity that is dependent upon Ca2+ [68]. In the present study, it was found that CCCP contrary to CPZ improved the activity of the four extracts (DES, HEL, UGL, and UGB) (Tables 5 and 6). This indicates that ABC transporters are involved in the resistance of the studied strains of S. aureus and that combination of extracts such as HEL, UGL, and UGB with an inhibitor of ABC transporters could improve the antistaphylococcal fight.


## 4.4. Antibiotic Modulation Effects of Extracts

The antibiotic resistance-modulating effects of several botanicals and phytochemicals against resistant bacteria have been documented [12–15, 59, 69]. It has been suggested that extracts capable of potentiating the activity of antibiotics on more than 70% of bacteria could be potential efflux pump inhibitors [70]. In this study, antibiotic modulation activity of extracts at their MIC/2 on more than 70% tested strains of S. aureus was obtained with the association of DEB and DEL and with 1/8 (12.5%) tested antibiotics (Tables S2 and S3; Supplementary Materials), UGB with 2/8 (25%) antibiotics (Table S6; Supplementary Materials), DES with 4/8 (50%) antibiotics (Table S4; Supplementary Materials), and RHL with 6/8 (75%) antibiotics (Table S5; Supplementary Materials). Hence, the tested extracts and mostly RHL may act as efflux pump inhibitors [70]. The use of CCCP indicated that ABC transporters were the efflux pumps involved in the resistance of the tested bacteria, suggesting that the above extract could be the inhibitors of such pumps. The potential of the R. heudelotii leaf extract (RHL) to reverse antibiotic resistance in Gram-negative MDR bacteria was previously reported [18]. The present study therefore provides more information about the ability of this plant to modulate the activity of antibiotics against resistant strains of S. aureus.


## 5. Conclusion

In conclusion, the present work provides informative data about the antistaphylococcal potential of 13 Cameroonian food plants. It also indicates that some extracts such as DES, HEL, UGL, and UGB could be used in combination with EPI to combat resistance ofStaphylococcus aureus to antibiotics. Finally, this study also demonstrates that some studied extracts and mostly RHL could be used as antibiotic resistance modulators, providing a new weapon against the resistance of S. aureus to antibiotics.



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*Source: 1920198-2018-07-03.xml*