# Syringocystadenocarcinoma Papilliferum In Situ: Case Report with Immunohistochemical Observations

Authors: Christian F. Lindboe; Hilde Brekke; Irina Schønhardt; Unn Houge
Journal: Scholarly Research Exchange
 (2009)

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## Abstract

A 23-year-old man was operated for a skin tumor in the right axilla. Histological examination revealed findings consistent with a syringocystadenoma papilliferum. However, most of the papillary structures showed a disorganized proliferation of atypical cells without invasive growth, that is, a syringocystadenocarcinoma papilliferum in situ. Immunohistochemistry disclosed a different staining profile between the basal cells of the papillary structures and those of the columnar and atypical cells which reacted similarly. However, the atypical cells showed a stronger and more extensive expression of S-100 protein and CD56 than the columnar epithelium and a few of them also reacted with CEA. Ki-67 staining highlighted the increased proliferative activity of the atypical cells. The best antibody for depicting the basal cells was p63. In the present case immunohistochemistry was of little help to support the diagnosis of malignant transformation since the differences between the atypical and regular columnar cells were small and of a quantitative character only.


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## Body

## 1. Introduction

Syringocystadenoma
papilliferum (SP) is a benign  skin adnexal tumor of apocrine or eccrine
derivation. The tumor is mainly seen in the head and neck  region and it may be
present at birth [1]. About 40% are associated with nevus sebaceous in the same
area, some of them developing a basal cell carcinoma as well [1]. There are
only few reports of transformation of an SP into an infiltrating
syringocystadenocarcinoma papilliferum (SPCA) [2–10] and even fewer cases
showing an SPCA in situ (SPCIS) [4, 5, 11–13].
The latter tumors are characterized by a disorganized proliferation of atypical
cells in the papillary structures of an SP but without tumor infiltration of the
surrounding dermis. Some of the reported cases with SPCA have revealed findings
consistent with SPCIS in the noninfiltrating cystic component indicating that
this change probably precedes infiltrative tumor growth [2–5, 7, 14]. There are
only few reports of immunohistochemical findings in SP and its malignant forms.
In this paper we report a case of SPCIS with application of a broad panel of
antibodies relevant for classification of epithelial tumors. In particular, we
focus on the different staining profiles between the two cell layers of the ordinary
papillary structures and those of the proliferating atypical cells.


## 2. Case Report and Histopathological Findings

The reported patient is a man
born in 1984 with a negative family history regarding inheritable and skin
diseases. In 1994 a granulation polyp was removed from his right external ear. Since childhood he
has had a skin tumor in the right axilla which increased somewhat in size and
became irritating during puberty. The lesion was removed in October 2008. The
specimen was described as a pale skin nodule measuring 11 × 8 mm with a central
pore. Microscopical examination revealed a well-demarcated cystic tumor opening
to the skin surface (Figure1(a)). In the superficial part the cyst was lined by a
hyperkeratotic squamous epithelium, in the deeper part by one layer of cuboidal
or columnar cells with multiple papillary projections into the cyst lumen. Some
of these papillae showed the typical picture of a benign syringocystadenoma
papilliferum with a two-layered epithelium resting upon a connective tissue
core heavily infiltrated with plasma cells. The inner basal cell layer was
cuboidal, the outer columnar (Figure 1(b), inset). However, most of the papillae
displayed a disorganized proliferation of cells with eosinophilic cytoplasm and
enlarged nuclei and nucleoli as well as scattered mitoses (Figures 1(b) and 1(c)). The
surrounding dermis showed neither tumor infiltration nor changes of a nevus
sebaceous. The resection margins were free.(a) Low magnification showing well-demarcated cystic lesion opening to the skin surface (hematoxylin and eosin stain). (b) Medium magnification displaying widespread cell proliferation of the papillae. Inset: papillary structure with typical two-layered epithelium (hematoxylin and eosin stain). (c) High magnification showing disorganized proliferation of cells with enlarged nuclei and nucleoli as well as scattered mitoses (hematoxylin and eosin stain). (d) Anti-p63 stain showing positive nuclear staining of basal cells. (e) Anti-CD56 stain with strong staining reaction of proliferating cells. Inset: weak and focal staining of columnar cells in an ordinary papilla. (f) Anti-Ki-67 stain showing high proliferative activity in atypical cells. Inset: low proliferative activity in ordinary two-layered epithelium.
(a)(b)(c)(d)(e)(f)The tumor was
immunohistochemically stained with a panel of antibodies to demonstrate
differences in staining properties between the ordinary two-layered papillary
epithelium and the atypical cells. The antibodies applied and the staining
characteristics of the various cell types are displayed in Table1. As can be
seen, the basal cells had a staining profile different from the columnar and
atypical cells which, on the other hand, showed a similar reaction. Thus, the
basal cells stained positively for smooth muscle actin and p63 (Figure 1(d));
negatively for Ber-Ep4, CK8, EMA, and CD56 in contrast to the columnar and
atypical cells. Furthermore, the basal cells gave a stronger staining reaction
for CK5/6 than the other two cell types. Generally, the atypical cells revealed
a stronger and more extensive staining for CD56 (Figure 1(e)) and S-100 protein than
the columnar cells and a few of them also expressed CEA. Ki-67 staining confirmed
the high proliferation activity of the atypical cells (≈50%) in contrast
to the much lower activity in the basal and columnar cells (Figure 1(f)).Table 1
Immunohistochemical
staining reactions of the various types of epithelia in the reported case of syringocystadenocarcinoma
papilliferum in situ.
AntibodySourceTypical two-layered
epitheliumAtypical cellsBasal cellsColumnar cellsAE1/AE3Dako+++++++++BCCKDako+++++++++Ber-Ep4Dako−+/+++/++CK5/6Dako+++++++CK7Dako+++++CK8Zymed−++++++EMADako−++++P63Dako+++−−CEADako−−+CD56Nordic biosite−++++Chr.ADako−−−SYNNovocastra−−−S-100Dako−−/++/++TTF-1Novocastra−−−SMADako++−−VIMDako++++ERDako−−−PGRDako−−−Ki-67Dako≈10%≈3%≈50%+++ =
strong staining reaction, ++ = moderate staining reaction, + = weak or focal staining reaction, BCCK =
basal cell cytokeratin, EMA = epithelial membrane antigen, CEA =
carcinoembryonic antigen, Chr.A =
chromogranin A, SYN = synaptophysin, S-100 =  S-100 protein, TTF-1 = thyroid transcription factor-1, SMA = smooth muscle actin, VIM = vimentin, ER = estrogen receptor, PGR = progesterone receptor.


## 3. Discussion

The reported tumor fulfils all
criteria for a syringocystadenoma papilliferum (SP). However, it also revealed
an intracystic proliferation of atypical cells without infiltration of the
dermis in conformity with lesions previously reported as “syringocystadenocarcinoma
papilliferum in situ” (SPCIS) [5, 12, 13].
There is probably a continuum from a benign SP via various grades of atypia to
an infiltrating syringocystadenocarcinoma papilliferum (SPCA), but criteria for
grading of these rare tumors have to our knowledge not been determined. Whereas
an SPCA may metastasize to regional lymph nodes [2, 14], this is not the case for
SPCIS which is cured by total surgical resection.The tumor  of our patient was
located at the  axilla which apparently is a rare location for SPs. In a survey
of 145 cases with benign SPs, Mammino and Vidmar [1] found no tumor at this
site whereas one previously reported case of SPCIS had an axillary location
like ours [4]. However, the number of such lesions is too small to suggest that
the axilla represents a predilection site for malignant transformation of SPs.There are only few reports on
immunohistochemical findings in SPs and their malignant forms, and little
attention has been paid to demonstrate staining differences between the various
cell types. Benign SPs have been reported to be positive for CEA [11, 12, 15, 16],
CK AE1 [12], and EMA [11], but variable for S-100 protein [11, 12]. Ansai et al.
[8] employed a large panel of antibodies in a case of SPCA comparing the staining
properties of the upper noninfiltrating and the deeper infiltrating parts of
the tumor. They found that both components reacted positively for CEA, S-100
protein and several cytokeratins including CK8. However, only the infiltrating
part stained positively for EMA. Thus, their findings are in discrepancy to
ours. In cases of SPCIS, Arai et al. [12] found positive staining for CK AE1,
variable for CEA, and negative for S-100 protein whereas Ishida-Yamamoto et
al. case [11] was positive for EMA, partly positive for S-100 protein and
negative for CEA in accordance with our findings. However, classification of
the latter tumor as an SPCIS must be questioned since the authors state that
“double-layered cuboidal and columnar epithelia typical of syringocystadenoma
papilliferum were not seen in this tumor.” Furthermore, the somewhat divergent
immunohistochemical findings between the various studies may partly be
explained by the use of different staining procedures and different antibody
clones.In conclusion, the immunohistochemical
staining panel applied in the present study was of little help to support the
diagnosis of malignant transformation in our case of SP since the differences
in staining properties between the ordinary columnar and the atypical cells
were of a quantitative character only. Further studies are necessary before
this matter can be definitely settled. A Ki-67 stain will, however, highlight
the increased proliferation activity of the atypical cells. Furthermore, p63 proved
to be the best antibody for visualizing the basal cells.  In case of an SPCIS, we will recommend to
examine sections cut at several levels of the blocks to rule out an
infiltrating tumor component.



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*Source: 176837-2009-04-05.xml*