# Peroxisome Proliferator-Activated Receptor Delta:  A Conserved Director of Lipid Homeostasis through Regulation of the Oxidative Capacity of Muscle

Authors: Pieter de Lange; Assunta Lombardi; Elena Silvestri; Fernando Goglia; Antonia Lanni; Maria Moreno
Journal: PPAR Research
 (2008)

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## Abstract

The peroxisome proliferator-activated receptors (PPARs), which are ligand-inducible transcription factors expressed in a variety of tissues, have been shown to perform key roles in lipid homeostasis. In physiological situations such as fasting and physical exercise, one PPAR subtype, PPARδ, triggers a transcriptional program in skeletal muscle leading to a switch in fuel usage from glucose/fatty acids to solely fatty acids, thereby drastically increasing its oxidative capacity. The metabolic action of PPARδ has also been verified in humans. In addition, it has become clear that the action of PPARδ is not restricted to skeletal muscle. Indeed, PPARδ has been shown to play a crucial role in whole-body lipid homeostasis as well as in insulin sensitivity, and it is active not only in skeletal muscle (as an activator of fat burning) but also in the liver (where it can activate glycolysis/lipogenesis, with the produced fat being oxidized in muscle) and in the adipose tissue (by incrementing lipolysis). The main aim of this review is to highlight the central role for activated PPARδ in the reversal of any tendency toward the development of insulin resistance.


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## Body

## 1. Introduction

The modern  Western lifestyle is
characterized by excessive food intake and lack of physical exercise. This has led to obesity caused by a
disturbance of lipid  homeostasis, becoming one of the most prevalent and
serious global chronic disorders. In lean organisms, fatty acids derived either
from food or from hepatic lipogenesis are utilized as energy substrates by the
heart and skeletal muscles. A strict physiological equilibrium between lipid
availability and lipid consumption needs to be maintained to prevent development
of both an impairment of insulin responsiveness and a metabolic dysfunction. In
adult obesity and obesity-associated metabolic disorders (which have strict
associations with type 2 diabetes, hypertension, and hyperlipidemia, and are often
referred to as metabolic syndrome [1]), a disturbance of lipid
homeostasis causes excess fat accumulation in various tissues (predominantly in
adipose tissues, but also in other insulin-responsive organs, such as skeletal
muscle and liver).Skeletal muscle is
quantitatively the largest organ in the body, and it contributes 30–40% of the
resting metabolic rate in adults. It is a major site for the oxidation of fatty
acids and glucose (accounting for approximately 80% of insulin-stimulated
glucose uptake), and it exhibits a remarkable flexibility in its usage of fuel.
One notable aspect of skeletal muscle plasticity is the specificity of its structural, biochemical, and functional adaptations to a given
stimulus [2]. In view of the above features, it is perhaps not surprising that
the predominant feature of type 2 diabetes is insulin resistance in skeletal
muscle [3, 4].The peroxisome proliferator-activated
receptors (PPARs), which are ligand-inducible transcription factors expressed in
a variety of tissues, have been shown to perform key roles in lipid
homeostasis. In physiological situations such as fasting [5] and physical
exercise [6], one PPAR subtype, PPARδ, triggers a transcriptional program in skeletal muscle leading to a switch
in fuel usage from glucose/fatty acids to solely fatty acids, and thereby
drastically increasing this tissue’s oxidative capacity. In addition, recent
evidence has highlighted the possibility that activating PPARδ in human subjects could increase skeletal muscle’s oxidative capacity
and so reverse metabolic abnormalities [7]. In mouse models, PPARδ has been shown to play a crucial role in whole-body lipid homeostasis
as well as in insulin sensitivity, and to be predominantly active in skeletal
muscle (as an activator of fat burning [8, 9]) but also in the liver (where it
can activate glycolysis/lipogenesis, with the produced fat being oxidized in
muscle [10]) and in the adipose tissue (by incrementing lipolysis [11]). Thus PPARδ activation provides a multiorgan “energy substrate-switching” phenotype
that triggers tissue-specific transcriptional programs, and in which skeletal
muscle plays a crucial role by reducing fat content.From the current
data presented in the literature, a central role for activated PPARδ can be deduced in skeletal muscle, liver, and white adipose
tissue (increased fat oxidation in muscle, increased carbohydrate catabolism
and fat synthesis in the liver, and increased lipolysis in white adipose
tissue) in the reversal of any tendency toward the development of insulin
resistance. Following a brief description of the structure, the mode of
activation and the action of the PPARs, this review aims to highlight the
multiorgan energy
switching role of PPARδ and its ultimate impact on insulin resistance. Figure 1 represents  an
overview of the central role played by PPARδin the use of fatty acids as
fuel by enhancing: (a) their oxidation in skeletal muscle (described in Section  3), (b) their synthesis from glucose in
the liver and their subsequent release (described in Section 4), and (c) their
release from the white adipose tissue (described in Section 5).Figure 1
Central, fuel-switching
mechanisms by which PPARδ increases the use of fatty acids in skeletal muscle without
provoking insulin resistance. Dotted arrow: indirect effect. For abbreviations,
the reader is referred to the text.


## 2. PPARs, Nuclear Receptors Activated by Fatty Acids

PPARs
are nuclear receptors that act as ligand-inducible transcription factors. The
three known isoforms PPARα, β(also termed
δ) , and γdisplay tissue-specific
expressions and possess different gene-regulatory profiles. PPARγ is a key regulator of adipose development and adipose insulin
sensitivity [12], whereas PPARα-regulated genes are involved in hepatic lipid oxidation [13]. The PPARδ isoform, the function of which has only recently been elucidated and
will be discussed in detail in this review, is predominantly expressed in
skeletal muscle (where it induces fatty acid oxidation), but it is also
expressed in brain, heart, liver, adipose tissue, and small intestine [14, 15].It is generally known that PPARs heterodimerize
with the retinoid X-receptor (RXR) and bind to a specific DNA sequence, (a
sequence termed peroxisome proliferators response element (PPRE), that is found
in a variety of genes involved in lipid and carbohydrate metabolism,
inflammation, and cell proliferation and differentiation) [16, 17]. However,
alternative mechanisms exist. Recently, an interplay has been reported between
PPARδ and thyroid hormone receptor β(TRβ) in the activation of the gene encoding uncoupling protein 3 (UCP3) [18]
in rat skeletal muscle as well as in cotransfection experiments in rat L6
myoblasts containing a reporter construct driven by the rat UCP3 promoter.
Activation of UCP3 gene transcription in vivo by thyroid hormone (T3) requires
the presence of fatty acids, while in the absence of fatty acids this
transcription can be restored by the PPARδ agonist L165,041 [18]. The UCP3 gene promoter has been shown to contain
a noncanonical thyroid hormone response element (TRE) termed TRE1 that is conserved
from rodents to humans [18, 19], and this response element is also recognized
by PPARs [19]. Interestingly, fatty acid responsiveness after T3 treatment was
only observed in cells transfected with the rat UCP3 promoter, which suggests a
species-specific regulation [18].Dietary fatty acids and fatty acid derivatives are
the natural ligands of PPARs, which display the greatest preference for
monounsaturated and polyunsaturated fatty acids (MUFAs and PUFAs, resp.), as
demonstrated by means of various ligand-binding assays [20, 21]. The fact that
each PPAR activates a different gene program, despite their overlapping
expressions, would seem to suggest ligand-specificity for each PPAR. Indeed, the structure of the ligand-binding pocket differs considerably among
the various PPARs as revealed by X-ray crystal-structure analysis [21, 22]. Nevertheless,
natural fatty acids can be ligands of all three PPAR isoforms. It is well known
that binding of the ligand promotes a conformational change that is permissive
for interactions with tissue-specific coactivator proteins, allowing nucleosome
remodelling and activation of the transcription of cell type-specific target
genes [21, 23]. It is, therefore, conceivable that a given fatty acid induces
different conformational changes when binding to the ligand-binding pockets of
the various PPAR subtypes. Given that the transcriptional activity induced by
each PPAR subtype is cell type-specific [24], the different conformations induced
following ligand binding may determine the cell-specificity of the different
PPARs (through heterodimerization with different receptors and binding to
cell-type specific cofactors). However, further research is needed to establish
which natural ligands might activate each PPAR in a given cellular
context.


## 3. Role of PPARδ in Skeletal Muscle: A Switch to Fat Oxidation

### 3.1. PPARδ-Induced Fuel Switching Evidenced by Its Overexpression or Ablation and by the Use of Synthetic Ligands in Rodents

In skeletal muscle, the relatively high expression of PPARδ (at 10- and 50-fold higher levels than PPARα and PPARγ, resp.; [15, 25]) as well as its preferential expression in
oxidative rather than glycolytic myofibers [9], has led to the suggestion that
this receptor isoform may be involved in promoting the utilization of fatty
acids as fuel. Indeed, treatment of rat L6 cells with the highly specific PPARδ agonist GW0742 has been shown to increase fatty acid
oxidation and induce expression of several lipid regulatory genes [26]. In
addition, treatment of C2C12 cells with GW0742 [27] or with another the PPARδ-specific agonist
(GW1516 [28]) induced expressions of genes involved in lipid catabolism and
energy uncoupling in skeletal muscle cells [29]. However, although an in vivo
magnetic resonance spectroscopy (MRS) study of rats treated with the latter
agonist revealed increased lipid metabolism, no change in mitochondrial energy coupling was detected efficiency despite an
increased UCP3 expression [29]. Skeletal muscle has the capacity to adapt its structure to
metabolic changes, as indicated by changes in the myosin type and a subsequent
shift in the number and type of muscle fibers. An increased demand for fat
metabolism is reflected by a shift in fiber-type away from the fast, glycolytic
form (type II) toward the slow-oxidative form (type I) [2]. PPARδ has emerged as an important stimulus in the induction of this fiber shift.
Indeed, studies
on transgenic mice harboring a constitutively activated form of PPARδ(VP16-PPARδ) have clearly shown an increase in skeletal muscle lipid
metabolism as well as the formation of type I fibers [9]. Mice overexpressing
peroxisome proliferator-activated receptor γcoactivator 1 α(PGC-1α), which activates PPARδ via a direct protein-protein interaction [30], have been
shown to possess a high content of type I fibers [31]. However, experiments on mice overexpressing
VP16-PPARδ have revealed that type I fiber formation can
be directly stimulated by PPARδ without an induction of PGC-1α [9]. Nevertheless, in physiological situations, an upregulation of
PGC-1α is likely to be involved in the early events leading to muscle-fiber
shifts, since PGC-1α is a coactivator of the PPARs. Indeed, PGC-1α-null mice show clear muscle dysfunction [32], and evidence that PPARδ induces an expression of PGC-1αhas come
from treatment of mice
with GW1516 [8] as well as from a study in which skeletal muscle C2C12 myocytes
transfected with a PGC-1α promoter-driven reporter construct were treated with the same ligand [33]. A wild-type PPARδ transgene (specifically overexpressed in mouse skeletal
muscle, but not constitutively activated) has been shown to promote a net increase in the number of
fibers with an oxidative metabolic capability through an increase in fiber
numbers in soleus and tibialis anterior muscle, while in plantaris muscle the
increase was more closely related to a shift from glycolytic to more oxidative
fibers [34].
Interestingly, this nonactivated PPARδtransgene failed to
induce the formation of type I fibers in skeletal muscle [34]. In this case,
despite its high expression, actual activation of the transgene would depend on
the presence of natural ligands, such as fatty acids. As the fatty acid concentrations fluctuate,
fatty regulation of PPARδ transcription is likely to be highly regulated.This raises the
question as to whether, instead of overexpression, increasing the availability
of ligand (natural or artificial) would induce fuel switching as well as fiber switching
toward type I fibers. Indeed, a fuel-switching role of PPARδ activation has been demonstrated by incubating rat isolated
skeletal muscle strips with the agonist GW1516 for 24 hours [35]. This led to
an increased use of fatty acids over glucose, as reflected by increased fatty
acid oxidation and reductions in glucose oxidation, glycogen synthesis, lactate
release, and glucose transport. Interestingly, this switch was independent of
insulin stimulation or fiber type [35]. The PPARδ agonist GW610742 also induced this fuel switch and in
addition triggered a genetic program toward initiation of muscular atrophy
without compromising mitochondrial activity in rat skeletal muscle [36]. Furthermore,
a somatic gene transfer of PPARδ into adult rat fibers has recently been shown to lead
to activation of fuel switching as well
as to a shift in the existing fiber profile toward the “slow,” oxidative
phenotype, an effect that caused the number of type I fibers in the extensor
digitorum longus (EDL) muscle to be tripled after 14 days [37]. From these
experiments, it is clear that PPARδ rapidly programs all fiber types in skeletal muscle to
oxidize fatty acids.


### 3.2. Evidence Supporting a Role for Activated PPARδ in Mediating Fuel Switching in Human Skeletal Muscle: Data from in vitro and Clinical Studies

Recent studies have revealed clear roles for PPARδ in the regulation of lipid and glucose metabolism in human
skeletal muscle [7, 38]. PPARδ stimulates the
expression of genes involved in (a)
increasing of lipid oxidation (fatty acid binding protein 3 (FABP3) and CPT1)
and (b) reducing carbohydrate oxidation
(pyruvate dehydrogenase kinase 4 (PDK4)) in human skeletal muscle [38], as it
does in rodents [5, 8–10]. These in vitro human skeletal muscle data
with GW1516
support the long-known observation that an increase in fatty acid oxidation
reduces the glucose utilization of isolated muscle reflecting the mutual
inhibition in the metabolism of substrates involved in the glucose-fatty acid
cycle [35, 36, 39]. Recent data from a clinical study indicate that this situation
holds true in human subjects. A statistically significant reduction in fasting
insulin levels was observed in a study performed on moderately obese men given
a dose of 10 mg o.d. of GW1516 was given for 2 weeks [7]. These subjects also
showed decrease fasting plasma nonesterified fatty acid (NEFA) concentrations
and increased expression of CPT1b in muscle. Interestingly, GW1516 treatment
slightly reduced the expression of PPARδ, showing that overexpression of the receptor itself is not necessary for the induction of
PPARδ-mediated effects, the resident levels of the receptor
protein being sufficient. In contrast, similar treatment with a PPARα agonist (GW590735) did not result in any change in plasma
insulin levels, nor did it cause a significant increase in CPT1b mRNA [7].


### 3.3. PPARδ, a Regulator of Fuel Use in Skeletal Muscle under Physiological Conditions

Since
fasting promotes increased utilization of fatty acids, fasting might also
regulate PPARδ expression. However, the initial reports appearing in the literature did not seem to
confirm this. For instance, a 24-hour fasting period was found not to
upregulate PPARδ in rat skeletal muscle [40], and our group even reported reduced
levels of PPARδ (and PPARα) in gastrocnemius muscle from 48-hour-fasted rats [25].
Decreased expressions of PPARδ and PPARα upon 48 hours of fasting have recently been reported in
humans, too [41]. In contrast, in mice, skeletal muscle PPARδ levels were found to be upregulated after a 24-hour fast [42].
One explanation is that studying the responses to physiological stimuli by making
single time point measurements may give misleading results, due to possible
transient modifications. Indeed, time course studies have shown that PPARδ actually is upregulated in fasted rat skeletal muscle, but
within the first 6 hours [43]. This upregulation of PPARδ (and of PGC-1α) during fasting is, however, transient [43], downregulation (after
the initial upregulation at 6 hours to around or below the control levels being
evident at 48 hours). Data showing that rapid nuclear accumulations of both
PGC-1α and PPARδupon food deprivation [43],
and their physical interaction within the nucleus [30], occur concomitantly
with increases in fatty acid levels and an increase in the expression of myosin heavy
chain Ib (MHC Ib), underline the role of PPARδ as a key regulator of fatty acid metabolism and muscle fiber
switching (in concert with its coactivator, PGC-1α).Following the
nuclear accumulation of PPARδ, its target genes (such as carnitine palmitoyl transferase 1b
(CPT1b), mitochondrial thioesterase I (MTE I), and UCP3) are upregulated
simultaneously, and so is the rate of mitochondrial fatty acid oxidation [43]. In starved mice, the mRNA for a
member of the FOXO family, FKHR (forkhead homolog in rhabdomyosarcoma), is
upregulated rapidly and transiently (starting within 6 hours, peaking at 12 hours,
and decreasing at 24 hours), and this is followed by an upregulation of FKHR
protein and a nuclear accumulation of nonphosphorylated FKHR levels with a
consequent upregulation of its target gene pyruvate dehydrogenase kinase 4
(PDK4) [44]. In mouse skeletal muscle, PDK4 has been shown to be a target of
PPARδ, since it is activated in vivo by GW1516 [8]. This kinase plays an
important role in the switching from glucose usage to fat usage since it
phosphorylates the E1 component of the pyruvate dehydrogenase (PDH) complex,
thereby downregulating carbohydrate (CH) oxidation [45]. Similarly, in human
skeletal muscle, PDK4 mRNA and protein levels have been found to be elevated at
both 24 hours and 48 hours of fasting, whereas at these time points the FOXO1
mRNA/protein levels were unchanged [41]. However, it is possible that
regulation of the mRNA/protein levels of FOXO1 (a target of PGC-1α [46], which are transiently regulated during fasting in rats [40]) is a
transient event preceding upregulation of PDK4 mRNA and protein in humans, too.
If so, such transient regulatory changes may have been missed by measuring only
at 24 hours and 48 hours of starvation in humans. At the 48-hour time point of
starvation, PPARδ mRNA levels in the human vastus lateralis muscle were decreased [41], a
finding in line with the decreased levels of PPARδmRNA [25] and nuclear protein [43]
at this time point in fasting rat gastrocnemius muscle. In the rat, this
decline was preceded by a rapid rise in the mRNA and protein levels of this
transcription factor, elevated levels being detected at 6 hours and 12 hours of
food deprivation [43]. Unfortunately, these time points were not investigated
in the human study [41]. Thus kinetic studies in the fasting situation, in
rodents as well as in humans, have made it clear that the expression of PPARδ, and that of its target genes, is tightly regulated. These results
offer an explanation for the rapid structural and metabolic changes favoring an
increased use of lipids as fuel that occur in this condition.The reported kinetics of the PPARδ upregulation occurring during the recovery period after exercise-imposed
energy stress in humans are in line with those seen in the fasting rat; a
single exhaustive bout of cycling increasing PPARδ mRNA and protein expression within 3 hours after completion
of the exercise [47, 48]. Regular physical training, which elevates the levels
of PPARδ and PGC-1α, leads to increases in both mitochondrial capacity and
insulin sensitivity [34, 49–51], effects
similar to those seen after PPARδ activation. The observations made in exercise and fasting experiments showing that PPARδ-expression kinetics are rapid, and the changes almost
immediate, are supported by PPARδ acutely bringing about the fuel switching effects inpre-existing
adult rat muscle fibers (as demonstrated by short-term agonist treatment [35, 36]
and somatic PPARδ gene transfer [36]). Recently, a direct role for PPARδ in the suppression of glucose oxidation in fasting skeletal
muscle has been shown by Nahlé et al. [52]. First, the authors demonstrated
that deficiency of the fatty acid translocase CD36 (mediating muscle fatty acid
uptake during fasting, [5]) blunts fasting induction of FOXO1 and PDK4 and the
associated suppression of glucose oxidation in mouse skeletal muscle [52]. Next, they demonstrated that loss of
PPARδ abolishes the fasting induction of muscle FOXO1 and PDK4 in vivo [52]. As
the authors identified several PPRE sites in the FOXO1 promoter, these results
suggest that CD36-dependent
activation of PPARδ results in the trascriptional regulation of FOXO1 as well as
PDK4 in fasted mice.Taken together, the
control of fuel use by activated PPARδ has emerged to be crucial for the rapid metabolic adaptation
of skeletal muscle to energy stress.


## 3.1. PPARδ-Induced Fuel Switching Evidenced by Its Overexpression or Ablation and by the Use of Synthetic Ligands in Rodents

In skeletal muscle, the relatively high expression of PPARδ (at 10- and 50-fold higher levels than PPARα and PPARγ, resp.; [15, 25]) as well as its preferential expression in
oxidative rather than glycolytic myofibers [9], has led to the suggestion that
this receptor isoform may be involved in promoting the utilization of fatty
acids as fuel. Indeed, treatment of rat L6 cells with the highly specific PPARδ agonist GW0742 has been shown to increase fatty acid
oxidation and induce expression of several lipid regulatory genes [26]. In
addition, treatment of C2C12 cells with GW0742 [27] or with another the PPARδ-specific agonist
(GW1516 [28]) induced expressions of genes involved in lipid catabolism and
energy uncoupling in skeletal muscle cells [29]. However, although an in vivo
magnetic resonance spectroscopy (MRS) study of rats treated with the latter
agonist revealed increased lipid metabolism, no change in mitochondrial energy coupling was detected efficiency despite an
increased UCP3 expression [29]. Skeletal muscle has the capacity to adapt its structure to
metabolic changes, as indicated by changes in the myosin type and a subsequent
shift in the number and type of muscle fibers. An increased demand for fat
metabolism is reflected by a shift in fiber-type away from the fast, glycolytic
form (type II) toward the slow-oxidative form (type I) [2]. PPARδ has emerged as an important stimulus in the induction of this fiber shift.
Indeed, studies
on transgenic mice harboring a constitutively activated form of PPARδ(VP16-PPARδ) have clearly shown an increase in skeletal muscle lipid
metabolism as well as the formation of type I fibers [9]. Mice overexpressing
peroxisome proliferator-activated receptor γcoactivator 1 α(PGC-1α), which activates PPARδ via a direct protein-protein interaction [30], have been
shown to possess a high content of type I fibers [31]. However, experiments on mice overexpressing
VP16-PPARδ have revealed that type I fiber formation can
be directly stimulated by PPARδ without an induction of PGC-1α [9]. Nevertheless, in physiological situations, an upregulation of
PGC-1α is likely to be involved in the early events leading to muscle-fiber
shifts, since PGC-1α is a coactivator of the PPARs. Indeed, PGC-1α-null mice show clear muscle dysfunction [32], and evidence that PPARδ induces an expression of PGC-1αhas come
from treatment of mice
with GW1516 [8] as well as from a study in which skeletal muscle C2C12 myocytes
transfected with a PGC-1α promoter-driven reporter construct were treated with the same ligand [33]. A wild-type PPARδ transgene (specifically overexpressed in mouse skeletal
muscle, but not constitutively activated) has been shown to promote a net increase in the number of
fibers with an oxidative metabolic capability through an increase in fiber
numbers in soleus and tibialis anterior muscle, while in plantaris muscle the
increase was more closely related to a shift from glycolytic to more oxidative
fibers [34].
Interestingly, this nonactivated PPARδtransgene failed to
induce the formation of type I fibers in skeletal muscle [34]. In this case,
despite its high expression, actual activation of the transgene would depend on
the presence of natural ligands, such as fatty acids. As the fatty acid concentrations fluctuate,
fatty regulation of PPARδ transcription is likely to be highly regulated.This raises the
question as to whether, instead of overexpression, increasing the availability
of ligand (natural or artificial) would induce fuel switching as well as fiber switching
toward type I fibers. Indeed, a fuel-switching role of PPARδ activation has been demonstrated by incubating rat isolated
skeletal muscle strips with the agonist GW1516 for 24 hours [35]. This led to
an increased use of fatty acids over glucose, as reflected by increased fatty
acid oxidation and reductions in glucose oxidation, glycogen synthesis, lactate
release, and glucose transport. Interestingly, this switch was independent of
insulin stimulation or fiber type [35]. The PPARδ agonist GW610742 also induced this fuel switch and in
addition triggered a genetic program toward initiation of muscular atrophy
without compromising mitochondrial activity in rat skeletal muscle [36]. Furthermore,
a somatic gene transfer of PPARδ into adult rat fibers has recently been shown to lead
to activation of fuel switching as well
as to a shift in the existing fiber profile toward the “slow,” oxidative
phenotype, an effect that caused the number of type I fibers in the extensor
digitorum longus (EDL) muscle to be tripled after 14 days [37]. From these
experiments, it is clear that PPARδ rapidly programs all fiber types in skeletal muscle to
oxidize fatty acids.


## 3.2. Evidence Supporting a Role for Activated PPARδ in Mediating Fuel Switching in Human Skeletal Muscle: Data from in vitro and Clinical Studies

Recent studies have revealed clear roles for PPARδ in the regulation of lipid and glucose metabolism in human
skeletal muscle [7, 38]. PPARδ stimulates the
expression of genes involved in (a)
increasing of lipid oxidation (fatty acid binding protein 3 (FABP3) and CPT1)
and (b) reducing carbohydrate oxidation
(pyruvate dehydrogenase kinase 4 (PDK4)) in human skeletal muscle [38], as it
does in rodents [5, 8–10]. These in vitro human skeletal muscle data
with GW1516
support the long-known observation that an increase in fatty acid oxidation
reduces the glucose utilization of isolated muscle reflecting the mutual
inhibition in the metabolism of substrates involved in the glucose-fatty acid
cycle [35, 36, 39]. Recent data from a clinical study indicate that this situation
holds true in human subjects. A statistically significant reduction in fasting
insulin levels was observed in a study performed on moderately obese men given
a dose of 10 mg o.d. of GW1516 was given for 2 weeks [7]. These subjects also
showed decrease fasting plasma nonesterified fatty acid (NEFA) concentrations
and increased expression of CPT1b in muscle. Interestingly, GW1516 treatment
slightly reduced the expression of PPARδ, showing that overexpression of the receptor itself is not necessary for the induction of
PPARδ-mediated effects, the resident levels of the receptor
protein being sufficient. In contrast, similar treatment with a PPARα agonist (GW590735) did not result in any change in plasma
insulin levels, nor did it cause a significant increase in CPT1b mRNA [7].


## 3.3. PPARδ, a Regulator of Fuel Use in Skeletal Muscle under Physiological Conditions

Since
fasting promotes increased utilization of fatty acids, fasting might also
regulate PPARδ expression. However, the initial reports appearing in the literature did not seem to
confirm this. For instance, a 24-hour fasting period was found not to
upregulate PPARδ in rat skeletal muscle [40], and our group even reported reduced
levels of PPARδ (and PPARα) in gastrocnemius muscle from 48-hour-fasted rats [25].
Decreased expressions of PPARδ and PPARα upon 48 hours of fasting have recently been reported in
humans, too [41]. In contrast, in mice, skeletal muscle PPARδ levels were found to be upregulated after a 24-hour fast [42].
One explanation is that studying the responses to physiological stimuli by making
single time point measurements may give misleading results, due to possible
transient modifications. Indeed, time course studies have shown that PPARδ actually is upregulated in fasted rat skeletal muscle, but
within the first 6 hours [43]. This upregulation of PPARδ (and of PGC-1α) during fasting is, however, transient [43], downregulation (after
the initial upregulation at 6 hours to around or below the control levels being
evident at 48 hours). Data showing that rapid nuclear accumulations of both
PGC-1α and PPARδupon food deprivation [43],
and their physical interaction within the nucleus [30], occur concomitantly
with increases in fatty acid levels and an increase in the expression of myosin heavy
chain Ib (MHC Ib), underline the role of PPARδ as a key regulator of fatty acid metabolism and muscle fiber
switching (in concert with its coactivator, PGC-1α).Following the
nuclear accumulation of PPARδ, its target genes (such as carnitine palmitoyl transferase 1b
(CPT1b), mitochondrial thioesterase I (MTE I), and UCP3) are upregulated
simultaneously, and so is the rate of mitochondrial fatty acid oxidation [43]. In starved mice, the mRNA for a
member of the FOXO family, FKHR (forkhead homolog in rhabdomyosarcoma), is
upregulated rapidly and transiently (starting within 6 hours, peaking at 12 hours,
and decreasing at 24 hours), and this is followed by an upregulation of FKHR
protein and a nuclear accumulation of nonphosphorylated FKHR levels with a
consequent upregulation of its target gene pyruvate dehydrogenase kinase 4
(PDK4) [44]. In mouse skeletal muscle, PDK4 has been shown to be a target of
PPARδ, since it is activated in vivo by GW1516 [8]. This kinase plays an
important role in the switching from glucose usage to fat usage since it
phosphorylates the E1 component of the pyruvate dehydrogenase (PDH) complex,
thereby downregulating carbohydrate (CH) oxidation [45]. Similarly, in human
skeletal muscle, PDK4 mRNA and protein levels have been found to be elevated at
both 24 hours and 48 hours of fasting, whereas at these time points the FOXO1
mRNA/protein levels were unchanged [41]. However, it is possible that
regulation of the mRNA/protein levels of FOXO1 (a target of PGC-1α [46], which are transiently regulated during fasting in rats [40]) is a
transient event preceding upregulation of PDK4 mRNA and protein in humans, too.
If so, such transient regulatory changes may have been missed by measuring only
at 24 hours and 48 hours of starvation in humans. At the 48-hour time point of
starvation, PPARδ mRNA levels in the human vastus lateralis muscle were decreased [41], a
finding in line with the decreased levels of PPARδmRNA [25] and nuclear protein [43]
at this time point in fasting rat gastrocnemius muscle. In the rat, this
decline was preceded by a rapid rise in the mRNA and protein levels of this
transcription factor, elevated levels being detected at 6 hours and 12 hours of
food deprivation [43]. Unfortunately, these time points were not investigated
in the human study [41]. Thus kinetic studies in the fasting situation, in
rodents as well as in humans, have made it clear that the expression of PPARδ, and that of its target genes, is tightly regulated. These results
offer an explanation for the rapid structural and metabolic changes favoring an
increased use of lipids as fuel that occur in this condition.The reported kinetics of the PPARδ upregulation occurring during the recovery period after exercise-imposed
energy stress in humans are in line with those seen in the fasting rat; a
single exhaustive bout of cycling increasing PPARδ mRNA and protein expression within 3 hours after completion
of the exercise [47, 48]. Regular physical training, which elevates the levels
of PPARδ and PGC-1α, leads to increases in both mitochondrial capacity and
insulin sensitivity [34, 49–51], effects
similar to those seen after PPARδ activation. The observations made in exercise and fasting experiments showing that PPARδ-expression kinetics are rapid, and the changes almost
immediate, are supported by PPARδ acutely bringing about the fuel switching effects inpre-existing
adult rat muscle fibers (as demonstrated by short-term agonist treatment [35, 36]
and somatic PPARδ gene transfer [36]). Recently, a direct role for PPARδ in the suppression of glucose oxidation in fasting skeletal
muscle has been shown by Nahlé et al. [52]. First, the authors demonstrated
that deficiency of the fatty acid translocase CD36 (mediating muscle fatty acid
uptake during fasting, [5]) blunts fasting induction of FOXO1 and PDK4 and the
associated suppression of glucose oxidation in mouse skeletal muscle [52]. Next, they demonstrated that loss of
PPARδ abolishes the fasting induction of muscle FOXO1 and PDK4 in vivo [52]. As
the authors identified several PPRE sites in the FOXO1 promoter, these results
suggest that CD36-dependent
activation of PPARδ results in the trascriptional regulation of FOXO1 as well as
PDK4 in fasted mice.Taken together, the
control of fuel use by activated PPARδ has emerged to be crucial for the rapid metabolic adaptation
of skeletal muscle to energy stress.


## 4. Antiglycemic Action of PPARδ in Liver:
Converting Glucose into Fat

At first glance, the above
described PPARδ-mediated fuel switching in skeletal muscle would be expected
to give rise to insulin resistance, since a preferential uptake and oxidation
of fatty acids (FFAs) over glucose (mediated by PPARδ stimulation in skeletal muscle) would lead to an accumulation
of blood glucose, muscle being a major player in blood-glucose homeostasis [1, 2].
However, as well as stimulating skeletal muscle fatty acid oxidation, PPARδ plays a surprising role in ameliorating hyperglycemia. It
does this by increasing hepatic glucose flux through the pentose phosphate
pathway and by enhancing fatty acid synthesis, the fatty acids being destined
for oxidization in muscle [10]. Whereas PPARδ-deprived mice are metabolically less active and glucose intolerant,
db/db mice treated with GW1516 show increased levels of CPT in muscle [10]
(similar effects of this agonist being reported in [8, 9]). This effect on CPT
is indicative of increased fatty acid oxidation, so it was a surprising finding
that gene array analysis revealed increased expression of gene clusters
involved in fatty acid synthesis and the pentose phosphate cycle in the liver
[7], both pathways using glucose or its metabolites as substrates. The authors
[10] suggested that PPARδ may increase glucose catabolism through these processes,
with the result that peripheral insulin
sensitivity may be improved [10]. Indeed, GW1516-treated db/db mice showed an
improved performance in the glucose tolerance test, and also showed a tendency
toward lowered fasting serum insulin levels [10].


## 5. Role for PPARδ in White Adipose Tissue: Increasing Lipolysis

Interestingly,
adipocyte hypertrophy induced by high-fat diet was accompanied by increased cannabinoid receptor type 1 (CB1R) expression and by a decrease
in PPARδ expression in adipose tissue [11]. Exercise attenuates adipocyte
hypertrophy and normalizes expression of CB1R and PPARδ. Functional cross-talk between CB1R and PPARδ is established by RNA interference experiments in 3T3-L1 preadipocytes.
For example, selective silencing of PPARδ by RNA interference significantly increased CB1R and increased adipocyte
differentiation and adenovirus-mediated
overexpression of PPARδ reduced CB1R expression [11]. The role of CB1R in obesity is well
established [53].
Depletion of CB1R in knockout mice is known (in animals fed a high-fat diet) to
reduce obesity through
increased lipolysis [53].The simultaneous regulation of skeletal muscle fatty
acid oxidation and adipose proliferation by PPARδ underlines the powerful role this receptor may play in preventing
weight gain in physiological situations.


## 6. Conclusions

Recent evidence shows that the fuel-switching
role of PPARδ in skeletal muscle is conserved from rodents to humans. This receptor
is crucial for a modulation of skeletal muscle flexibility that directs this
tissue toward the use of fatty acids as fuel, an effect with rapid kinetics. It
is becoming increasingly clear that even though the fat oxidation-inducing effect of
PPARδ is exerted in skeletal muscle, this receptor also activates transcriptional
programs in other tissues (such as adipose tissue, in which PPARδ directly inhibits proliferation and induces lipolysis), thereby channelling
the fat consumed in the diet directly to muscle for oxidation. In addition, in
an action that serves to prevent insulin resistance developing as a consequence
of a reduction of carbohydrate oxidation in muscle, the liver is activated to
form fat from glucose, thereby both allowing skeletal muscle to oxidize more
fat and concomitantly lowering blood glucose levels. PPARδ is emerging as a crucial nuclear receptor for the multiorgan regulation
of whole body fuel turnover, with skeletal muscle as the central organ in the
burning of fat and the consequent prevention or reduction of obesity (see Figure 1). These features make PPARδ a good candidate as a central target for the future treatment of
metabolic disturbances linked to obesity and insulin resistance.



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*Source: 172676-2008-09-22.xml*