# Suitability of Aquatic Plant Fibers for Handmade Papermaking

Authors: Nordiah Bidin; Muta Harah Zakaria; Japar Sidik Bujang; Nur Aznadia Abdul Aziz
Journal: International Journal of Polymer Science
 (2015)

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## Abstract

Increasing concerns for future fiber supplies in pulp and paper industries has shifted interest in nonwood sources from agriculture residues and aquatic plants. Aquatic plants with short growth cycles, in abundance, and with low lignin are a potential fiber source. Five aquatic plant species,Cyperus digitatus, Cyperus halpan, Cyperus rotundus, Scirpus grossus, and Typha angustifolia, were examined for fiber dimensions and chemical composition (cellulose, lignin) and compared with other nonwood plants. All aquatic plants possessed short (length, 0.71–0.83 mm) and thin (diameter, 9.13–12.11 µm) fibers, narrow lumen (diameter, 4.32–7.30 µm), and thin cell wall (thickness, 2.25–2.83 µm) compared with most other nonwood plants. Slenderness ratio ranged from 73.77 to 89.34 with Typha angustifolia having the highest ratio. Except for Scirpus grossus, the flexibility coefficient ranged from 52.91 to 58.08. Scirpus grossus has low Runkel ratio, 0.84 ± 0.17. Fiber characteristics, short and thin fibers, Slenderness ratio >60, flexibility coefficient within 50–75, and Runkel ratio <1, are suitable for papermaking. Cellulose content of Cyperus rotundus (42.58 ± 1.32%), Scirpus grossus (36.21 ± 2.81%), and Typha angustifolia (44.05 ± 0.49%) >34% is suitable for pulp and papermaking. Lignin content in aquatic plants in the present study ranged 9.54–20.04% and below the wood lignin content of <23–30% encountered in pulp and papermaking. Handmade paper sheets produced for paperboard, craft, and decorative purposes are with permissible tensile strength, breaking length, and low moisture content.


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## Body

## 1. Introduction

Population growth, better literacy, and development of communication and industrialization in developing countries increase worldwide paper products demand continuously [1]. In 2005, 17.4 million metric tons (9.27%) of nonwood fibers from 187.6 million metric tons global production of virgin pulp for paper and paperboard are produced [2]. In many countries, quantities of available wood are insufficient to meet the requirements and demands of pulp and paper especially in Mediterranean countries like Spain, Italy, and Greece [3–5]. In Malaysia, over one million tons of papers were produced in 2005 [6]. This would mean that more tropical trees need to be felled to sustain papermaking industry to meet the paper requirement and demand. To reduce the loss of rainforests, an attempt was made to find the alternative source of fiber for paper. Some alternatives have been used to replace the wood fiber with nonwood derived fibers [1] from agriculture residues such as wheat and rice straw, sorghum stalks, jute, and hemp for paper production [7, 8].Another alternative source of fibers for paper production is from aquatic plants found in lakes, ditches, rivers, ponds, and estuaries. They have short life cycle, grow massively, and due to their abundance can cause problems in irrigation channels water bodies [9, 10]. Besides, excessive growth of these aquatic plants can influence water management and ecosystem in ways such as affecting drainage, aesthetics, fishing activities, flood control, irrigation, and recreational and land values [11]. One way to control these plants is to use them as an alternative source for papermaking. Aquatic plants are nonfood plants and they have large differences in their physical and chemical characteristics [12, 13]. According to Ververis et al. [5], fiber dimension, lignin, and cellulose content of nonwood plants determine their suitability for paper production. Under certain conditions, tearing resistance of the paper is highly dependent on fiber dimension, that is, fiber length [14]. Although the major source of fiber for paper production is from wood plant, nonwood aquatic plants with less lignin content have potentials to be used as an alternative fiber source [13].Handmade paper industry is an environmental friendly and very promising industry for local entrepreneurship. Papers made from aquatic plants have multiple uses. They can be used for writing, food wrapping, tissue paper, and book mark and can be commercialized as value added materials for handmade crafts. Hence, the objectives of this study was to examine the fiber characteristics, derived values, and chemical composition of five aquatic plant species and compare them with other nonwood plant species that have been used to produce pulp and paper to determine their suitability for handmade papermaking. Additionally, handmade paper sheets produced of selected aquatic plants were tested for their quality with respect to tensile strength, breaking length, and moisture content.


## 2. Materials and Methods

### 2.1. Sample Collection

Aquatic plants,Cyperus digitatus,Cyperus halpan,Cyperus rotundus,Scirpus grossus andTypha angustifolia, found in abundance in ponds (N 02° 59.109′, E 101° 41.432′) at Universiti Putra Malaysia and wetland areas (N 02° 59.163′, E 101° 39.000′) around Selangor, Malaysia, were chosen and collected for this study. Plants were cleaned, leaves were removed, and stems were kept for determination of fiber dimension and derived values. Fresh stems were dried for determination of chemical composition and papermaking as described in detail below.


### 2.2. Fiber Dimension

Stems of aquatic plant were chopped into small pieces of 1 to 3 cm in length using a knife. One gram (1 g) of stems was placed in a test tube and macerated with 10 mL 33.5% nitric acid (HNO3) and boiled in water bath at 80°C for 2 hours. Macerated stems containing fibers were rinsed with distilled water to remove yellow stains of HNO3 and placed in a small flask containing 50 mL distilled water. A drop of macerated fiber suspension was placed on microscope slide and a drop of Safranin was used to enhance the cell wall visibility. Fiber diameter, fiber length, cell wall thickness, and lumen diameter were viewed and measured, and image was captured and recorded under calibrated Axioskop NIKON compound microscope. The fiber dimensions determination was following the method of Ververis et al. [5]. All fiber dimensions were used to determine the derived values, slenderness ratio, flexibility coefficient, and Runkel ratio, following Tamolang [15] and calculated as shown below.Slenderness ratio = fiber length (μm)/fiber diameter (μm).Flexibility coefficient = fiber lumen diameter (μm)/fiber diameter (μm) × 100.Runkel ratio = cell wall thickness (μm)/fiber lumen diameter (μm) × 2.


### 2.3. Chemical Composition

Of the five aquatic plant species,Cyperus rotundus,Scirpus grossus, andTypha angustifolia were chosen based on their fiber dimensions and derived values (lower and higher values as determined from Section 2.2 above) for evaluation of their chemical composition. The stems were ground and sieved with 250 μm sieve. Two grams (2 g) of sample was weighted and placed in cellulose thimble and the cellulose was extracted by using Soxhlet apparatus with 2 : 1 alcohol acetone solution. After 4 hours of extraction, sample was placed in desiccators until obtaining the constant weight. The cellulose content determination was performed in triplicates. The cellulose, hemicelluloses, and lignin contents were calculated based on the formula described by Moubasher et al. [16] as follows.Cellulose content =(Y-Z)/W×100.Hemicelluloses content =(X-Y)/W×100.Lignin content =(Z-V)/W×100.V = weight of glass thimble (g), W = weight of extractive-free sample (g), Y = weight of glass thimble with sample after being treated with KOH (g), and Z = weight of the glass thimble with sample after being hydrolyzed with H2SO4 (g).


### 2.4. Pulping and Papermaking

Hundred gram (100 g) of dried stems ofCyperus rotundus,Scirpus grossus, andTypha angustifolia was cooked with 20 g sodium carbonate (Na2CO3) and 2 L of water at 140°C for 2 hours by using induction cooker. After 2 hours, the cooked samples were cleaned under water flow to remove the chemical and blended by using electronic blender. 50 mL of starch solution (1 g of starch diluted in 1000 mL of distilled water) was then added to the pulp in an electronic mixture. Mould and deckle pouring method and couching technique was used in the papermaking process according to Hiebert [17].


### 2.5. Paper Quality

Moisture content of the produced paper sheet was determined by placing one gram (1 g) of paper sheet on AD-4715 Infrared Moisture Determination Balance. The tensile strength and breaking length were tested for the paper sheet strips by using modified TAPPI (Technical Association of the Pulp and Paper Industry) 494 om-06 [18] standard method of tensile properties for paper and paperboard. Tensile strength and breaking length were calculated as shown below.Tensile strength (kN/m) = Maximum breaking force (kN)/width of paper strip (m).Breaking length (km) = 102 000 × (Tensile strength (kN/m)/grammage (g/m2).


### 2.6. Statistical Analysis

One way analysis of variance (ANOVA) followed by post hoc Duncan’s multiple range test (p<0.05) were conducted using SPSS program to compare aquatic plants species fiber dimensions, derived values, chemical composition, tensile strength, and breaking length. Principle Component Analysis (PCA) based on Bray Curtis similarity index was carried out using XLSTAT software (Windows version 2013) to obtain the relationship between fiber dimensions, derived values, and chemical composition of aquatic plant species in this study with other nonwood plant species that have been used to produce different type of papers.


## 2.1. Sample Collection

Aquatic plants,Cyperus digitatus,Cyperus halpan,Cyperus rotundus,Scirpus grossus andTypha angustifolia, found in abundance in ponds (N 02° 59.109′, E 101° 41.432′) at Universiti Putra Malaysia and wetland areas (N 02° 59.163′, E 101° 39.000′) around Selangor, Malaysia, were chosen and collected for this study. Plants were cleaned, leaves were removed, and stems were kept for determination of fiber dimension and derived values. Fresh stems were dried for determination of chemical composition and papermaking as described in detail below.


## 2.2. Fiber Dimension

Stems of aquatic plant were chopped into small pieces of 1 to 3 cm in length using a knife. One gram (1 g) of stems was placed in a test tube and macerated with 10 mL 33.5% nitric acid (HNO3) and boiled in water bath at 80°C for 2 hours. Macerated stems containing fibers were rinsed with distilled water to remove yellow stains of HNO3 and placed in a small flask containing 50 mL distilled water. A drop of macerated fiber suspension was placed on microscope slide and a drop of Safranin was used to enhance the cell wall visibility. Fiber diameter, fiber length, cell wall thickness, and lumen diameter were viewed and measured, and image was captured and recorded under calibrated Axioskop NIKON compound microscope. The fiber dimensions determination was following the method of Ververis et al. [5]. All fiber dimensions were used to determine the derived values, slenderness ratio, flexibility coefficient, and Runkel ratio, following Tamolang [15] and calculated as shown below.Slenderness ratio = fiber length (μm)/fiber diameter (μm).Flexibility coefficient = fiber lumen diameter (μm)/fiber diameter (μm) × 100.Runkel ratio = cell wall thickness (μm)/fiber lumen diameter (μm) × 2.


## 2.3. Chemical Composition

Of the five aquatic plant species,Cyperus rotundus,Scirpus grossus, andTypha angustifolia were chosen based on their fiber dimensions and derived values (lower and higher values as determined from Section 2.2 above) for evaluation of their chemical composition. The stems were ground and sieved with 250 μm sieve. Two grams (2 g) of sample was weighted and placed in cellulose thimble and the cellulose was extracted by using Soxhlet apparatus with 2 : 1 alcohol acetone solution. After 4 hours of extraction, sample was placed in desiccators until obtaining the constant weight. The cellulose content determination was performed in triplicates. The cellulose, hemicelluloses, and lignin contents were calculated based on the formula described by Moubasher et al. [16] as follows.Cellulose content =(Y-Z)/W×100.Hemicelluloses content =(X-Y)/W×100.Lignin content =(Z-V)/W×100.V = weight of glass thimble (g), W = weight of extractive-free sample (g), Y = weight of glass thimble with sample after being treated with KOH (g), and Z = weight of the glass thimble with sample after being hydrolyzed with H2SO4 (g).


## 2.4. Pulping and Papermaking

Hundred gram (100 g) of dried stems ofCyperus rotundus,Scirpus grossus, andTypha angustifolia was cooked with 20 g sodium carbonate (Na2CO3) and 2 L of water at 140°C for 2 hours by using induction cooker. After 2 hours, the cooked samples were cleaned under water flow to remove the chemical and blended by using electronic blender. 50 mL of starch solution (1 g of starch diluted in 1000 mL of distilled water) was then added to the pulp in an electronic mixture. Mould and deckle pouring method and couching technique was used in the papermaking process according to Hiebert [17].


## 2.5. Paper Quality

Moisture content of the produced paper sheet was determined by placing one gram (1 g) of paper sheet on AD-4715 Infrared Moisture Determination Balance. The tensile strength and breaking length were tested for the paper sheet strips by using modified TAPPI (Technical Association of the Pulp and Paper Industry) 494 om-06 [18] standard method of tensile properties for paper and paperboard. Tensile strength and breaking length were calculated as shown below.Tensile strength (kN/m) = Maximum breaking force (kN)/width of paper strip (m).Breaking length (km) = 102 000 × (Tensile strength (kN/m)/grammage (g/m2).


## 2.6. Statistical Analysis

One way analysis of variance (ANOVA) followed by post hoc Duncan’s multiple range test (p<0.05) were conducted using SPSS program to compare aquatic plants species fiber dimensions, derived values, chemical composition, tensile strength, and breaking length. Principle Component Analysis (PCA) based on Bray Curtis similarity index was carried out using XLSTAT software (Windows version 2013) to obtain the relationship between fiber dimensions, derived values, and chemical composition of aquatic plant species in this study with other nonwood plant species that have been used to produce different type of papers.


## 3. Results and Discussion

### 3.1. Fiber Dimensions and Derived Values

The fiber lengths of the aquatic plants ranged from 0.71 to 0.83 mm and are relatively shorter than those of other nonwood plants (Table1). Of the five species,Scirpus grossus possessed longer fiber length (0.83±0.02 mm), wide fiber diameter (12.11±0.98 μm), lumen diameter (7.30±0.89 μm), higher flexibility coefficient (58.08±4.07), and low Runkel ratio (0.84±0.17). Fiber length ofScirpus grossus is comparable with crop plants,Zea mays (0.88 mm). Besides fiber length, fiber diameter, lumen diameter, and cell wall thickness of nonwood plants also varied depending on the plant species and the parts (leaves, stems) from which the fibers are derived (Table 1) and this supported the observation made by Ilvessalo-Pfaffli [19]. As a comparison with hard-wood plant such asPopulous tremuloides for kraft pulp, the fiber lengths are longer, 1.0–1.3 mm, and are reported to be suitable for coated paper production [20]. However, longer fiber length tends to give less fine of sheet structure [21, 22].Table 1
Comparison of fiber dimension and derived values of aquatic and other nonwood plant species.
Species
Part
Fiber dimension
Derived value
Reference (s)
Fiber length (mm)
Fiber diameter (µm)
Lumen diameter (µm)
Cell wall thickness (µm)
Slenderness ratio
Flexibility coefficient
Runkel ratio
(1)Cyperus digitatus
S
0.72 ± 0.03b
9.64 ± 0.39bc
5.15 ± 0.40bc
2.25 ± 0.14b
76.85 ± 4.31ab
52.91 ± 2.85ab
1.06 ± 0.14ab
Present study
(2)Cyperus rotundus
S
0.71 ± 0.02b
9.13 ± 0.47c
4.32 ± 0.37c
2.41 ± 016ab
81.57 ± 4.95ab
46.63 ± 2.56bc
1.28 ± 0.13ab
Present study
(3)Cyperus halpan
S
0.73 ± 0.04b
11.08 ± 0.55ab
6.02 ± 0.53ab
2.53 ± 0.18ab
69.01 ± 4.52b
53.54 ± 3.11ab
1.02 ± 0.15a
Present study
(4)Scirpus grossus
S
0.83 ± 0.02a
12.11 ± 0.98a
7.30 ± 0.89a
2.41 ± 0.16ab
73.77 ± 7.32ab
58.08 ± 4.07a
0.84 ± 0.17a
Present study
(5)Typha angustifolia
S
0.83 ± 0.02a
10.01 ± 0.66bc
4.35 ± 0.42c
2.83 ± 0.18a
89.34 ± 5.62a
42.52 ± 2.19c
1.52 ± 0.18b
Present study
(6)Eichhornia crassipes
Lf
1.60
5.50
9.00
2.50
290.90
163.64
0.56
Goswami and Saikia [23]
(7)Arundo donax
In
1.22
17.30
8.50
4.40
70.50
49.20
1.00
Ververis et al. [5]
(8)Musa paradisiaca
S
1.55
22.00
14.20
5.50
70.50
64.55
0.77
Goswami et al. [24]
(9)Saccharum sp.
Bg
1.51
21.40
6.27
7.74
70.56
29.29
2.46
Agnihotri et al. [25]
(10)Zea mays
Rs
0.88
20.12
10.92
4.59
44.08
54.27
0.84
Kiaei et al. [26]
(11)Bambusa tulda
St
1.89
17.00
3.45
6.78
111.20
20.29
3.93
Sharma et al. [27]
(12)Brassica napus
St
1.20
13.10
8.60
2.25
91.00
64.00
0.58
Tofanica et al. [22]
All values are given as mean ± S.E. Alphabets in the same column indicate significant difference atp < 0.05 (DMRT), a > b > c. Aquatic plants (no. 1–7); crop plants (no. 8–10); commercial plants (no. 11); vegetable plants (no. 12). S: stem; Lf: leaf; In: internode; Bg: bagasse; Rs: residue; St: stalk.Derived values (slenderness ratio, flexibility coefficient, and Runkel ratio) measure the ability of fibers to bind each other in the paper sheet. Slenderness ratio for aquatic plant species studied ranged from 77 to 89.34, a ratio >60 which is attributed to the thin fibers (cf. with other nonwood plants, Table1) suitable for producing high quality paper [5]. A combination of short and thin fibers usually will produce a good slenderness ratio, which is related to tearing resistance, paper sheet density, and pulp digestibility [28]. Comparatively, these values are close to slenderness ratio of 69.17–81.07 ofHibiscus cannabinus used to produce quality paper [29]. The trend of flexibility coefficient categorically placedScirpus grossus (58.08) as the highest followed byCyperus halpan (53.54) andCyperus digitatus (52.91) and these values are relatively high compared with other aquatic plant,Arundo donax internode (49.20), commercial plantBambusa tulda stalk (20.29), and crops plant ofSaccharum sp. baggase (29.29) and are comparable withZea mays residue (54.27) [5, 25–27]. Except forScirpus grossus,Cyperus halpan, andCyperus digitatus the flexibility coefficient (52.91–58.08) of aquatic plant species is within the preferable flexibility coefficient range of 50–75 [30]. Runkel ratio is good inScirpus grossus (0.84±0.17) andCyperus digitatus (1.06±0.14) compared with crop plants,Saccharum sp. (2.46) and commercial plant,Bambusa tulda (3.93) [25, 27]. The Runkel ratio >1 (e.g., 1.52±0.18 as inTypha angustifolia) indicated that it is less flexible and stiffer and that it forms bulkier paper [5]. Low Runkel ratio and high fiber length resulted in good pulp strength properties [31]. Runkel ratio <1 is related to high flexibility coefficient [32] and gives good mechanical strength properties to the paper produced [28].The principal component analysis (PCA) was performed to assess similarity in the fiber characteristics and derived values (slenderness ratio, flexibility coefficient, and Runkel ratio) of the aquatic plant species with other nonwood plants (Table1) that have been tested for paper production (Table 2). The obtained results based on Bray-Curtis similarity index at 50% similarity showed the total variance of the first two components is 74.50% (PC1 has a total variance of 45.16% and PC2 29.34%) and the species were clustered into five distinct groups (A, B, C, D, and E, Figure 1). Aquatic plant species,Cyperus digitatus,Cyperus halpan,Cyperus rotundus,Scirpus grossus, andTypha angustifolia, are clustered in only one group, C, independent of four other nonwood plant species, group A (Musa paradisiaca,Bambusa tulda,Saccharum sp., andArundo donax), group B (Zea mays), group D (Brassica napus), and group E (Eichhornia crassipes). Species in group B, D and E are suitable for production of writing or printing paper [5], composites and paperboard [22] and fiber plate, rigid cardboard and cardboard paper [26] (Table 2). According to Enayati et al. [1] and Kasmani et al. [33], a combination of nonwood/softwood and hardwood fibers can be promising and can have potential in papermaking.Table 2
Types of paper produced from aquatic and other nonwood plant species.
Species
Part
Parameter tested
Paper properties tested
Paper types
Reference (s)
(1)Eichhornia crassipes
Physical properties
Fiber dimension, derived value
Greaseproof paper
Goswami and Saikia [23]
Lf
Chemical properties
Cellulose, hemicelluloses, pentosan
Paper properties
Degree of freeness, burst index, tear index, tensile index
(2)Arundo donax
Physical properties
Fiber dimension, derived value
Printing and writing paper
In
Chemical properties
Cellulose, lignin, ash
Ververis et al. [5]
Paper properties
Slenderness ratio, tearing resistance
(3)Musa paradisiaca
S
Physical properties
Fiber dimension, derived value
Greaseproof paper
Goswami et al. [24]
Chemical properties
Cellulose, hemicelluloses, lignin, ash, silica
Paper properties
Burst index, tear index, tensile index, blister, double-fold number, degree of freeness
(4)Saccharum sp.
Physical properties
Fiber dimension, derived value
Writing and printing paper
Agnihotri et al. [25]
Bg
Chemical properties
Holocellulose, cellulose, hemicelluloses, ash, silica
Paper properties
Tensile index, tear index, burst index, double-fold number
(5)Zea mays
Rs
Physical properties
Fiber dimension, derived value
Fiber plate, rigid cardboard and cardboard paper
Kiaei et al. [26]
Chemical properties
Cellulose, lignin, ash
(6)Bambusa tulda
St
Physical properties
Fiber dimension, derived value
Writing and printing paper
Sharma et al. [27]
Chemical properties
Cellulose, holocellulose, lignin, ash
(7)Brassica napus
St
Physical properties
Fiber dimension, derived value
Composites, paper and paperboard
Tofanica et al. [22]
Chemical properties
Cellulose, holocellulose, pentosan, lignin, ash, silica
Aquatic plants (no. 1-2); crop plants (no. 3–5); commercial plants (no. 6); vegetable plants (no. 7). Lf: leaf;  In: internode;  S: stem;  Bg: bagasse;  Rs: residue;  St: stalk.Figure 1
Principal component analysis (PC1 and PC2) of five aquatic plant species compared with other nonwood plant species based on their fiber dimensions and derived values.


### 3.2. Chemical Composition

Among the species studied,Typha angustifolia possessed comparatively higher percentages of cellulose and hemicelluloses content, 44.05±0.49% and 54.84±4.27%, respectively. The cellulose content of all aquatic plant species was high and comparable with vegetable plants,Brassica napus (34.50%) and other aquatic plants,Arundo donax (36.70%), andTypha (pati) (36.80%) (Table 3). In addition, the percentage of cellulose content >40% was comparable withHibiscus cannabinus [5], used to produce quality paper [29]. The holocellulose, a combination of cellulose and hemicellulose amounts to >65–70% of reported plant dry weight [34]. The cellulose content >34% indicates the plants are suitable for pulp and paper manufacturing. Cellulose content affects the strength and makes the fiber strand liable to natural and synthetic dye binding while hemicelluloses is responsible for the water absorption by plant fibers and reduces internal fiber stress.Table 3
Chemical composition of aquatic and other nonwood plant species.
Species
Part
Chemical composition (%)
Types of paper produced
References
Cellulose
Hemicellulose
Lignin
(1)Cyperus  rotundus
S
42.58 ± 1.32a
45.64 ± 1.12a
9.54 ± 1.08b
Cardboard, paper and paperboard
Present study
(2)Scirpus grossus
S
36.21 ± 2.81b
49.88 ± 0.71a
13.44 ± 3.90a
Cardboard, paper and paperboard
Present study
(3)Typha angustifolia
S
44.05 ± 0.49a
54.84 ± 4.27a
20.04 ± 3.37a
Cardboard, paper and paperboard
Present study
(4)Typha (pati)
Wh
36.80
n/a
16.20
Cottage industry
Jahan et al. [35]
(5)Arundo donax
In
36.70
n/a
18.50
Printing and writing paper
Ververis et al. [5]
(6)Musa paradisiaca
S
59.18
n/a
18.21
Greaseproof paper
Goswami et al. [24]
(7)Zea mays
Rs
47.33
n/a
21.33
Fiber plate, rigid cardboard and cardboard paper
Kiaei et al. [26]
(8)Bambusa tulda
St
47.00
n/a
25.70
Printing and writing paper
Sharma et al. [27]
(9)Brassica napus
St
34.50
n/a
20.60
Composites, paper and paperboard
Tofanica et al. [22]
Mean in column with the different superscript (a > b > c) is significantly different (DMRT,p < 0.05) for present study. Aquatic plants (no. 1–5); crop plants (no. 6-7), commercial plants (no. 8); vegetable plants (no. 9). n/a: not available; S: stem; Wh: whole; In: internode; Rs: residue; St: stalk.Lignin content was higher inTypha angustifolia (20.04±3.37%) followed byScirpus grossus (13.44±3.90%) and it was lowest inCyperus rotundus (9.54±1.08%).Cyperus rotundus has the lower lignin content compared withTypha (pati) (16.20%) [35],Arundo donax (18.50%) [5],Musa paradisiaca (18.21%) [24], andZea mays (21.33%) [26]. Moreover, lignin content inTypha angustifolia was similar withBrassica napus (19.21–20%) [22, 36]. The lignin content for these studied species was lower than wood fiber lignin content of 23–30% for pulp and papermaking [13]. Dutt and Tyagi [28] reported that lignin content inEucalyptus sp. was >25%. However, all three species can be pulped in one-third of the time needed for hardwoods and softwood due to the lower lignin content [5]. Lignin was considered undesirable component during pulping and papermaking due to its unstable color and for being relatively dark and its hydrophobic surface caused unfavorable interfiber bond formation of hemicelluloses and cellulose [37].Comparison of chemical composition of aquatic plants and other nonwood plants (vegetables, crops, and commercial plants) with their type of paper is shown in Table3. The present study data and available data on nonwood plants were ordinated with PCA using lignin and cellulose composition. The biplot generated four main clusters (Figure 2). Aquatic plants are in two clusters:Typha angustifolia is in group B withZea mays andBambusa tulda whileCyperus rotundus andScirpus grossus are in group D. Based on fiber characteristics, cellulose and lignin content, plants in group B, can be utilized for production of fiber plate, rigid cardboard, cardboard paper, writing and content of printing paper (Table 3, [25, 27]). In group C, paper sheets derived from fibers and cellulose from these plants had been tested and were suitable for handmade paper in the cottage industry, composites, paperboard, and writing and printing paper (Table 3, [5, 22, 35]) for decorative purposes.Figure 2
Principal component analysis (PC1 and PC2) of three aquatic plant species compared with other nonwood plant species based on their cellulose and lignin composition.


### 3.3. Paper Quality

Cyperus rotundus has the highest tensile strength (1.69±0.18 kN/m) and breaking length (731.68±72.75 m) (Table 4). The tensile strength of paper sheets produced from aquatic plants,Cyperus rotundus,Scirpus grossus, andTypha angustifolia, in this present study is in the range of 0.94–1.69 kN/m and this reflected the intimate structure of paper [38]. Its individual fibers, their arrangement, and the extent to which they are bonded to each other are key factors which contribute to tensile strength. Long fibers generally produced paper with higher tensile strength properties than paper from short fiber. However, interfiber bonding is considered as the most important factor contributing to the paper tensile strength. Jeyasingam [39] mentioned that breaking length forHibiscus cannabinus was 4000 m ten times higher than the present study range of 410.11–731.68 m. Jahan et al. [40] also found that the breaking length of nonwood raw materials such as jute, cotton stalks, corn stalks, bagasse, saccharum, rice straw, and wheat straw varies in the range of 5511–7550 m. In addition, the breaking length values are in the range of 3650–5300 m for different types of paper, that is, offset, rag bond, and news print papers [38]. Hierarchically, paper moisture content wasTypha angustifolia (13.13±0.11%) >Scirpus grossus (13.08±0.41%) >Cyperus rotundus (10.11±0.042%). Moisture in paper varies from 2% to 12% depending on relative humidity, type of pulp used, degree of refining, and chemical used. Ideally a good quality paper possessed properties of comparatively high tensile strength and breaking length and lower moisture content. Other than being used for craft, wrapping, or decorative purposes, fibers derived from aquatic plant species as in this study may be suitable for newsprint production as their tensile strength is in the range of newsprint paper (0.90–1.79 kN/m) as reported by Caulfield and Gunderson [38].Table 4
Measurement for determination of paper quality of selected aquatic plant species.
Species
Tensile strength (kN/m)
Breaking length (m)
Moisture content (%)
Cyperus rotundus
1.69 ± 0.18a
731.68 ± 72.75a
10.11 ± 0.04b
Scirpus grossus
1.52 ± 0.21a
612.39 ± 34.05a
13.08 ± 0.41a
Typha angustifolia
0.94 ± 0.20b
410.11 ± 82.85b
13.13 ± 0.11a
All values are given as mean ± S.E. Different alphabets in the same column of parameter indicate significant difference atp < 0.05; that is, a > b.


## 3.1. Fiber Dimensions and Derived Values

The fiber lengths of the aquatic plants ranged from 0.71 to 0.83 mm and are relatively shorter than those of other nonwood plants (Table1). Of the five species,Scirpus grossus possessed longer fiber length (0.83±0.02 mm), wide fiber diameter (12.11±0.98 μm), lumen diameter (7.30±0.89 μm), higher flexibility coefficient (58.08±4.07), and low Runkel ratio (0.84±0.17). Fiber length ofScirpus grossus is comparable with crop plants,Zea mays (0.88 mm). Besides fiber length, fiber diameter, lumen diameter, and cell wall thickness of nonwood plants also varied depending on the plant species and the parts (leaves, stems) from which the fibers are derived (Table 1) and this supported the observation made by Ilvessalo-Pfaffli [19]. As a comparison with hard-wood plant such asPopulous tremuloides for kraft pulp, the fiber lengths are longer, 1.0–1.3 mm, and are reported to be suitable for coated paper production [20]. However, longer fiber length tends to give less fine of sheet structure [21, 22].Table 1
Comparison of fiber dimension and derived values of aquatic and other nonwood plant species.
Species
Part
Fiber dimension
Derived value
Reference (s)
Fiber length (mm)
Fiber diameter (µm)
Lumen diameter (µm)
Cell wall thickness (µm)
Slenderness ratio
Flexibility coefficient
Runkel ratio
(1)Cyperus digitatus
S
0.72 ± 0.03b
9.64 ± 0.39bc
5.15 ± 0.40bc
2.25 ± 0.14b
76.85 ± 4.31ab
52.91 ± 2.85ab
1.06 ± 0.14ab
Present study
(2)Cyperus rotundus
S
0.71 ± 0.02b
9.13 ± 0.47c
4.32 ± 0.37c
2.41 ± 016ab
81.57 ± 4.95ab
46.63 ± 2.56bc
1.28 ± 0.13ab
Present study
(3)Cyperus halpan
S
0.73 ± 0.04b
11.08 ± 0.55ab
6.02 ± 0.53ab
2.53 ± 0.18ab
69.01 ± 4.52b
53.54 ± 3.11ab
1.02 ± 0.15a
Present study
(4)Scirpus grossus
S
0.83 ± 0.02a
12.11 ± 0.98a
7.30 ± 0.89a
2.41 ± 0.16ab
73.77 ± 7.32ab
58.08 ± 4.07a
0.84 ± 0.17a
Present study
(5)Typha angustifolia
S
0.83 ± 0.02a
10.01 ± 0.66bc
4.35 ± 0.42c
2.83 ± 0.18a
89.34 ± 5.62a
42.52 ± 2.19c
1.52 ± 0.18b
Present study
(6)Eichhornia crassipes
Lf
1.60
5.50
9.00
2.50
290.90
163.64
0.56
Goswami and Saikia [23]
(7)Arundo donax
In
1.22
17.30
8.50
4.40
70.50
49.20
1.00
Ververis et al. [5]
(8)Musa paradisiaca
S
1.55
22.00
14.20
5.50
70.50
64.55
0.77
Goswami et al. [24]
(9)Saccharum sp.
Bg
1.51
21.40
6.27
7.74
70.56
29.29
2.46
Agnihotri et al. [25]
(10)Zea mays
Rs
0.88
20.12
10.92
4.59
44.08
54.27
0.84
Kiaei et al. [26]
(11)Bambusa tulda
St
1.89
17.00
3.45
6.78
111.20
20.29
3.93
Sharma et al. [27]
(12)Brassica napus
St
1.20
13.10
8.60
2.25
91.00
64.00
0.58
Tofanica et al. [22]
All values are given as mean ± S.E. Alphabets in the same column indicate significant difference atp < 0.05 (DMRT), a > b > c. Aquatic plants (no. 1–7); crop plants (no. 8–10); commercial plants (no. 11); vegetable plants (no. 12). S: stem; Lf: leaf; In: internode; Bg: bagasse; Rs: residue; St: stalk.Derived values (slenderness ratio, flexibility coefficient, and Runkel ratio) measure the ability of fibers to bind each other in the paper sheet. Slenderness ratio for aquatic plant species studied ranged from 77 to 89.34, a ratio >60 which is attributed to the thin fibers (cf. with other nonwood plants, Table1) suitable for producing high quality paper [5]. A combination of short and thin fibers usually will produce a good slenderness ratio, which is related to tearing resistance, paper sheet density, and pulp digestibility [28]. Comparatively, these values are close to slenderness ratio of 69.17–81.07 ofHibiscus cannabinus used to produce quality paper [29]. The trend of flexibility coefficient categorically placedScirpus grossus (58.08) as the highest followed byCyperus halpan (53.54) andCyperus digitatus (52.91) and these values are relatively high compared with other aquatic plant,Arundo donax internode (49.20), commercial plantBambusa tulda stalk (20.29), and crops plant ofSaccharum sp. baggase (29.29) and are comparable withZea mays residue (54.27) [5, 25–27]. Except forScirpus grossus,Cyperus halpan, andCyperus digitatus the flexibility coefficient (52.91–58.08) of aquatic plant species is within the preferable flexibility coefficient range of 50–75 [30]. Runkel ratio is good inScirpus grossus (0.84±0.17) andCyperus digitatus (1.06±0.14) compared with crop plants,Saccharum sp. (2.46) and commercial plant,Bambusa tulda (3.93) [25, 27]. The Runkel ratio >1 (e.g., 1.52±0.18 as inTypha angustifolia) indicated that it is less flexible and stiffer and that it forms bulkier paper [5]. Low Runkel ratio and high fiber length resulted in good pulp strength properties [31]. Runkel ratio <1 is related to high flexibility coefficient [32] and gives good mechanical strength properties to the paper produced [28].The principal component analysis (PCA) was performed to assess similarity in the fiber characteristics and derived values (slenderness ratio, flexibility coefficient, and Runkel ratio) of the aquatic plant species with other nonwood plants (Table1) that have been tested for paper production (Table 2). The obtained results based on Bray-Curtis similarity index at 50% similarity showed the total variance of the first two components is 74.50% (PC1 has a total variance of 45.16% and PC2 29.34%) and the species were clustered into five distinct groups (A, B, C, D, and E, Figure 1). Aquatic plant species,Cyperus digitatus,Cyperus halpan,Cyperus rotundus,Scirpus grossus, andTypha angustifolia, are clustered in only one group, C, independent of four other nonwood plant species, group A (Musa paradisiaca,Bambusa tulda,Saccharum sp., andArundo donax), group B (Zea mays), group D (Brassica napus), and group E (Eichhornia crassipes). Species in group B, D and E are suitable for production of writing or printing paper [5], composites and paperboard [22] and fiber plate, rigid cardboard and cardboard paper [26] (Table 2). According to Enayati et al. [1] and Kasmani et al. [33], a combination of nonwood/softwood and hardwood fibers can be promising and can have potential in papermaking.Table 2
Types of paper produced from aquatic and other nonwood plant species.
Species
Part
Parameter tested
Paper properties tested
Paper types
Reference (s)
(1)Eichhornia crassipes
Physical properties
Fiber dimension, derived value
Greaseproof paper
Goswami and Saikia [23]
Lf
Chemical properties
Cellulose, hemicelluloses, pentosan
Paper properties
Degree of freeness, burst index, tear index, tensile index
(2)Arundo donax
Physical properties
Fiber dimension, derived value
Printing and writing paper
In
Chemical properties
Cellulose, lignin, ash
Ververis et al. [5]
Paper properties
Slenderness ratio, tearing resistance
(3)Musa paradisiaca
S
Physical properties
Fiber dimension, derived value
Greaseproof paper
Goswami et al. [24]
Chemical properties
Cellulose, hemicelluloses, lignin, ash, silica
Paper properties
Burst index, tear index, tensile index, blister, double-fold number, degree of freeness
(4)Saccharum sp.
Physical properties
Fiber dimension, derived value
Writing and printing paper
Agnihotri et al. [25]
Bg
Chemical properties
Holocellulose, cellulose, hemicelluloses, ash, silica
Paper properties
Tensile index, tear index, burst index, double-fold number
(5)Zea mays
Rs
Physical properties
Fiber dimension, derived value
Fiber plate, rigid cardboard and cardboard paper
Kiaei et al. [26]
Chemical properties
Cellulose, lignin, ash
(6)Bambusa tulda
St
Physical properties
Fiber dimension, derived value
Writing and printing paper
Sharma et al. [27]
Chemical properties
Cellulose, holocellulose, lignin, ash
(7)Brassica napus
St
Physical properties
Fiber dimension, derived value
Composites, paper and paperboard
Tofanica et al. [22]
Chemical properties
Cellulose, holocellulose, pentosan, lignin, ash, silica
Aquatic plants (no. 1-2); crop plants (no. 3–5); commercial plants (no. 6); vegetable plants (no. 7). Lf: leaf;  In: internode;  S: stem;  Bg: bagasse;  Rs: residue;  St: stalk.Figure 1
Principal component analysis (PC1 and PC2) of five aquatic plant species compared with other nonwood plant species based on their fiber dimensions and derived values.


## 3.2. Chemical Composition

Among the species studied,Typha angustifolia possessed comparatively higher percentages of cellulose and hemicelluloses content, 44.05±0.49% and 54.84±4.27%, respectively. The cellulose content of all aquatic plant species was high and comparable with vegetable plants,Brassica napus (34.50%) and other aquatic plants,Arundo donax (36.70%), andTypha (pati) (36.80%) (Table 3). In addition, the percentage of cellulose content >40% was comparable withHibiscus cannabinus [5], used to produce quality paper [29]. The holocellulose, a combination of cellulose and hemicellulose amounts to >65–70% of reported plant dry weight [34]. The cellulose content >34% indicates the plants are suitable for pulp and paper manufacturing. Cellulose content affects the strength and makes the fiber strand liable to natural and synthetic dye binding while hemicelluloses is responsible for the water absorption by plant fibers and reduces internal fiber stress.Table 3
Chemical composition of aquatic and other nonwood plant species.
Species
Part
Chemical composition (%)
Types of paper produced
References
Cellulose
Hemicellulose
Lignin
(1)Cyperus  rotundus
S
42.58 ± 1.32a
45.64 ± 1.12a
9.54 ± 1.08b
Cardboard, paper and paperboard
Present study
(2)Scirpus grossus
S
36.21 ± 2.81b
49.88 ± 0.71a
13.44 ± 3.90a
Cardboard, paper and paperboard
Present study
(3)Typha angustifolia
S
44.05 ± 0.49a
54.84 ± 4.27a
20.04 ± 3.37a
Cardboard, paper and paperboard
Present study
(4)Typha (pati)
Wh
36.80
n/a
16.20
Cottage industry
Jahan et al. [35]
(5)Arundo donax
In
36.70
n/a
18.50
Printing and writing paper
Ververis et al. [5]
(6)Musa paradisiaca
S
59.18
n/a
18.21
Greaseproof paper
Goswami et al. [24]
(7)Zea mays
Rs
47.33
n/a
21.33
Fiber plate, rigid cardboard and cardboard paper
Kiaei et al. [26]
(8)Bambusa tulda
St
47.00
n/a
25.70
Printing and writing paper
Sharma et al. [27]
(9)Brassica napus
St
34.50
n/a
20.60
Composites, paper and paperboard
Tofanica et al. [22]
Mean in column with the different superscript (a > b > c) is significantly different (DMRT,p < 0.05) for present study. Aquatic plants (no. 1–5); crop plants (no. 6-7), commercial plants (no. 8); vegetable plants (no. 9). n/a: not available; S: stem; Wh: whole; In: internode; Rs: residue; St: stalk.Lignin content was higher inTypha angustifolia (20.04±3.37%) followed byScirpus grossus (13.44±3.90%) and it was lowest inCyperus rotundus (9.54±1.08%).Cyperus rotundus has the lower lignin content compared withTypha (pati) (16.20%) [35],Arundo donax (18.50%) [5],Musa paradisiaca (18.21%) [24], andZea mays (21.33%) [26]. Moreover, lignin content inTypha angustifolia was similar withBrassica napus (19.21–20%) [22, 36]. The lignin content for these studied species was lower than wood fiber lignin content of 23–30% for pulp and papermaking [13]. Dutt and Tyagi [28] reported that lignin content inEucalyptus sp. was >25%. However, all three species can be pulped in one-third of the time needed for hardwoods and softwood due to the lower lignin content [5]. Lignin was considered undesirable component during pulping and papermaking due to its unstable color and for being relatively dark and its hydrophobic surface caused unfavorable interfiber bond formation of hemicelluloses and cellulose [37].Comparison of chemical composition of aquatic plants and other nonwood plants (vegetables, crops, and commercial plants) with their type of paper is shown in Table3. The present study data and available data on nonwood plants were ordinated with PCA using lignin and cellulose composition. The biplot generated four main clusters (Figure 2). Aquatic plants are in two clusters:Typha angustifolia is in group B withZea mays andBambusa tulda whileCyperus rotundus andScirpus grossus are in group D. Based on fiber characteristics, cellulose and lignin content, plants in group B, can be utilized for production of fiber plate, rigid cardboard, cardboard paper, writing and content of printing paper (Table 3, [25, 27]). In group C, paper sheets derived from fibers and cellulose from these plants had been tested and were suitable for handmade paper in the cottage industry, composites, paperboard, and writing and printing paper (Table 3, [5, 22, 35]) for decorative purposes.Figure 2
Principal component analysis (PC1 and PC2) of three aquatic plant species compared with other nonwood plant species based on their cellulose and lignin composition.


## 3.3. Paper Quality

Cyperus rotundus has the highest tensile strength (1.69±0.18 kN/m) and breaking length (731.68±72.75 m) (Table 4). The tensile strength of paper sheets produced from aquatic plants,Cyperus rotundus,Scirpus grossus, andTypha angustifolia, in this present study is in the range of 0.94–1.69 kN/m and this reflected the intimate structure of paper [38]. Its individual fibers, their arrangement, and the extent to which they are bonded to each other are key factors which contribute to tensile strength. Long fibers generally produced paper with higher tensile strength properties than paper from short fiber. However, interfiber bonding is considered as the most important factor contributing to the paper tensile strength. Jeyasingam [39] mentioned that breaking length forHibiscus cannabinus was 4000 m ten times higher than the present study range of 410.11–731.68 m. Jahan et al. [40] also found that the breaking length of nonwood raw materials such as jute, cotton stalks, corn stalks, bagasse, saccharum, rice straw, and wheat straw varies in the range of 5511–7550 m. In addition, the breaking length values are in the range of 3650–5300 m for different types of paper, that is, offset, rag bond, and news print papers [38]. Hierarchically, paper moisture content wasTypha angustifolia (13.13±0.11%) >Scirpus grossus (13.08±0.41%) >Cyperus rotundus (10.11±0.042%). Moisture in paper varies from 2% to 12% depending on relative humidity, type of pulp used, degree of refining, and chemical used. Ideally a good quality paper possessed properties of comparatively high tensile strength and breaking length and lower moisture content. Other than being used for craft, wrapping, or decorative purposes, fibers derived from aquatic plant species as in this study may be suitable for newsprint production as their tensile strength is in the range of newsprint paper (0.90–1.79 kN/m) as reported by Caulfield and Gunderson [38].Table 4
Measurement for determination of paper quality of selected aquatic plant species.
Species
Tensile strength (kN/m)
Breaking length (m)
Moisture content (%)
Cyperus rotundus
1.69 ± 0.18a
731.68 ± 72.75a
10.11 ± 0.04b
Scirpus grossus
1.52 ± 0.21a
612.39 ± 34.05a
13.08 ± 0.41a
Typha angustifolia
0.94 ± 0.20b
410.11 ± 82.85b
13.13 ± 0.11a
All values are given as mean ± S.E. Different alphabets in the same column of parameter indicate significant difference atp < 0.05; that is, a > b.


## 4. Conclusion

Scirpus grossus,Cyperus rotundus, andTypha angustifolia are suitable aquatic plants species for papermaking based on their fiber characteristics, chemical composition, and physical properties. An abundance and availability of these plants can provide sustainable large biomass as raw fibers for pulp and paper production. Handmade paper sheets produced for paperboard, writing, and printing paper used for craft, wrapping, and decorative purposes are with permissible tensile strength, breaking length, and low moisture content.



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*Source: 165868-2015-10-25.xml*