# Enhanced Degradation of Lactide-co-Glycolide Polymer with Basic Nucleophilic Drugs

Authors: Susan D’Souza; Jabar A. Faraj; Rossella Dorati; Patrick P. DeLuca
Journal: Advances in Pharmaceutics
 (2015)

---

## Abstract

The purpose of this study was to examine the degradative effect of weakly basic nucleophilic drugs on a lactide-co-glycolide (PLGA) polymer in a microsphere formulation. Biodegradable PLGA microspheres of two second-generation atypical antipsychotics, Risperidone and Olanzapine, were manufactured using a solvent extraction/evaporation technique. The effect of drug content, buffer pH and temperature on polymer molecular weight and degradation, were examined via a series of experiments and compared against a control (Placebo PLGA microspheres). In comparison to Placebo microspheres, significant polymer molecular weight reduction was observed upon encapsulation of varying levels of either Risperidone or Olanzapine. There was excellent correlation between the extent of molecular weight reduction during manufacture and the amount of encapsulated drug in the microspheres. Subsequent studies on polymer degradation showed: the following (a) the Placebo and Olanzapine microspheres followed pseudo first order kinetics, (b) Risperidone microspheres exhibited biphasic degradation profiles, and (c) polymer degradation was dependent on temperature, not pH. The findings of these studies show that encapsulation of weakly basic nucleophile type drugs into PLGA can accelerate the biodegradation of the PLGA and have major implications on the design of polymeric microsphere drug delivery systems.


---

## Body

## 1. Introduction

Considerable advances in the areas of research involving biodegradable polymers as implantable reservoirs for sustained-release drug delivery have been realized over the past few decades [1–4]. A key feature that makes use of biodegradable polymers attractive in the medical sciences is the fact that they do not require surgical removal after completion of drug release. Among the most popular biodegradable polymers are polylactide (PLA) and poly(lactic-co-glycolide) (PLGA) polymers, which are known to be nontoxic, biocompatible, and nonimmunogenic.Generally, degradation of poly(esters) like PLA and PLGA occurs in four major stages:(a)
polymer hydration causing disruption of the primary and secondary structure due to hydrogen bonding and van der Waals forces,(b)
loss of mechanical strength caused by the rupture of covalent bonds of the polymer backbone to form oligomers with acidic end-groups,(c)
polymer mass loss due to diffusion of acidic oligomers resulting in accelerated water absorption,(d)
polymer dissolution and/or phagocytosis [5].As would be expected, polymer hydration of PLA or PLGA causes ester hydrolysis that has been shown to proceed via pseudo first order kinetics and is described as follows:(1)d[COOH]dt=kCOOH,
where [COOH] refers to the concentration of the carboxylic acid end group in the PLA or PLGA polymer and “k” is the rate constant. Eventually, hydrolytic degradation of the poly(ester) produces lactic and glycolic acids, both moieties clearedin vivoby the Krebs cycle.In addition to their use in surgical sutures and prosthetic devices, biodegradable poly(ester) polymers are also used in commercially available injectable products, for example, Risperdal Consta (Risperidone in PLGA) and Lupron Depot (Leuprolide acetate in PLGA) [6, 7]. Literature also cites examples of a number of therapeutic agents like small molecules, peptides, and proteins that have been successfully encapsulated into these polymers [8, 9]. Depending on the polymer type and the incorporated therapeutic agent, the polymeric dosage form can provide sustained drug levels for varying durationsin vivo[10, 11]. Indeed, research has shown that these therapeutic agents have varying physicochemical properties that could alter polymer degradation and thereby alter drug release profiles [12–16]. This highlights the need to investigate the effect of a drug on polymer degradation as it can be critical with respect to product performance, as cited by several authors [17–19].While the chemical composition of PLGA and PLA is essential for biodegradability and biocompatibility, a notable effect on the drug release profile is detected especially if the therapeutic agent is a basic drug. Such types of drugs are expected to interact with the acidic polymer thereby accelerating polymer degradation and influencing drug release from the polymer matrix. For instance, researchers have reported that tertiary amine drugs or nucleophilic compounds like local anesthetics accelerate the hydrolytic degradation of poly(D,L-lactide) polymer [20–22]. These aspects illustrate that studies involving design and development of a biodegradable microsphere dosage form must include studies on possible degradative effects of the drug on the polymer. Such studies will not only aid in the fundamental understanding of the dosage form but also enable advancements in the area of polymeric delivery systems.Towards this goal, the research presented in this publication explores the degradation in microsphere formulations containing basic drugs. The drugs chosen for the study are two popular second-generation atypical antipsychotics, Risperidone and Olanzapine. Both molecules are commonly prescribed for the treatment of schizophrenia and other psychotic disorders [23–26]. The structures of Risperidone and Olanzapine are shown in Figure 1. Both drugs are weak bases having pKa values of 8.24 and 7.37 for Risperidone and Olanzapine, respectively. Risperidone contains two tertiary amines while Olanzapine contains two tertiary amines and one secondary amine suggesting that the latter drug is a stronger base and nucleophile. The work reported in this publication details the approach taken during the development of microsphere formulations of Risperidone and Olanzapine and showcases the effects of drug loading, temperature, and pH on the degradation of a 65 : 35 PLGA polymer.Chemical structures of Risperidone and Olanzapine.
(a)
(b)The objectives of this study include:(i)
identification of any possible effects of Risperidone and Olanzapine on the PLGA polymer,(ii)
assessment of the influence of drug loading on polymer degradation,(iii)
determination of the impact of buffer and temperature on the polymer degradation process.The study also highlights the similarities and differences in behavior between Olanzapine and Risperidone and provides a framework for researchers investigating sustained dosage formulations that utilize biodegradable polymers.


## 2. Materials and Methods

### 2.1. Materials

Hydrochloride salts of Risperidone and Olanzapine were purchased from Cipla Ltd., Bombay, India, DL65 : 35 PLGA (MWinitial=75 kDa) from Medisorb, Dupont, Wilmington, DE, and Polyvinyl alcohol (PVA, MW 30–70 kDa) from Sigma, St. Louis, MO, USA. All other chemicals were obtained commercially as analytical grade reagents.


### 2.2. Preparation of Microspheres

Placebo, Risperidone, and Olanzapine PLGA microspheres were prepared by dispersing a homogenous solution of polymer and drug into an aqueous solution containing 0.35% Polyvinyl alcohol followed by solvent extraction/evaporation for 2 hours [27, 28]. The solidified microspheres were recovered by filtration and dried under vacuum at room temperature for 48 hours.


### 2.3. Drug Content

A 10 mg amount of microspheres was dissolved in 8 mL Acetonitrile followed by the addition of 32 mL of 0.1 M acetate buffer, pH 4.0. The contents were diluted with 80 : 20 Acetate buffer : acetonitrile mixture and filtered using 0.45μm Teflon filter, prior to analysis. The two drugs were assayed by reverse phase HPLC method using a Nucleosil 5 μ C18 column (250 × 4.6 mm; Phenomenex, Torrance, CA, USA), mobile phase: 70 : 30 water : acetonitrile with 0.1% TFA, flow rate = 1 mL/min at 275 nm.


### 2.4. Particle Size Distribution

Particles were sized by laser diffractometry using a Malvern 2600 laser sizer (Malvern 2600 particle sizer, Malvern, UK). The average particle size was expressed as the volume mean diameter (Vmd) in microns.


### 2.5. Degradation of Microspheres

50 mg of drug loaded microspheres or Placebo microspheres was transferred to a bottle containing 50 mL of 0.02 M phosphate buffer saline (PBS) pH 7.2 containing 0.05% Tween 80, at 37°C. At predetermined intervals, the remaining microspheres were filtered using 0.65μm PVDF filters (Millipore, USA). The microspheres were dried in a vacuum drier for at least 3 days to ensure low moisture content followed by determination of molecular weight.


### 2.6. Determination of Molecular Weight

The molecular weight distribution of Placebo and drug loaded microspheres was determined by gel permeation chromatography (GPC). The GPC system consisted of two Ultrastyragel columns connected in series (7.8 × 300 mm each, one with 104 Å pores and one with 103 Å pores), a delivery device (Shimadzu LC-6A, Japan), UV detector set at λ = 210 nm (Shimadzu, Japan), and software to compute molecular weight distribution (Waters, Maxima 820, Milford, USA). Sample solutions in tetrahydrofuran (THF) at a concentration of 5 mg/mL were filtered through a 0.45 μm filter (Millipore, USA) before injection into the GPC system and were eluted with THF at 0.4 mL/min. The weight-average molecular weight was calculated using monodisperse polystyrene standard where(i)
MWinitial is the initial polymer molecular weight (75 kDa),(ii)
MW0 represents the molecular weight after manufacture of Placebo or drug loaded microspheres (t-zero),(iii)
MWt at time “t=x days” is the molecular weight of a sample (Placebo or drug loaded microspheres).


## 2.1. Materials

Hydrochloride salts of Risperidone and Olanzapine were purchased from Cipla Ltd., Bombay, India, DL65 : 35 PLGA (MWinitial=75 kDa) from Medisorb, Dupont, Wilmington, DE, and Polyvinyl alcohol (PVA, MW 30–70 kDa) from Sigma, St. Louis, MO, USA. All other chemicals were obtained commercially as analytical grade reagents.


## 2.2. Preparation of Microspheres

Placebo, Risperidone, and Olanzapine PLGA microspheres were prepared by dispersing a homogenous solution of polymer and drug into an aqueous solution containing 0.35% Polyvinyl alcohol followed by solvent extraction/evaporation for 2 hours [27, 28]. The solidified microspheres were recovered by filtration and dried under vacuum at room temperature for 48 hours.


## 2.3. Drug Content

A 10 mg amount of microspheres was dissolved in 8 mL Acetonitrile followed by the addition of 32 mL of 0.1 M acetate buffer, pH 4.0. The contents were diluted with 80 : 20 Acetate buffer : acetonitrile mixture and filtered using 0.45μm Teflon filter, prior to analysis. The two drugs were assayed by reverse phase HPLC method using a Nucleosil 5 μ C18 column (250 × 4.6 mm; Phenomenex, Torrance, CA, USA), mobile phase: 70 : 30 water : acetonitrile with 0.1% TFA, flow rate = 1 mL/min at 275 nm.


## 2.4. Particle Size Distribution

Particles were sized by laser diffractometry using a Malvern 2600 laser sizer (Malvern 2600 particle sizer, Malvern, UK). The average particle size was expressed as the volume mean diameter (Vmd) in microns.


## 2.5. Degradation of Microspheres

50 mg of drug loaded microspheres or Placebo microspheres was transferred to a bottle containing 50 mL of 0.02 M phosphate buffer saline (PBS) pH 7.2 containing 0.05% Tween 80, at 37°C. At predetermined intervals, the remaining microspheres were filtered using 0.65μm PVDF filters (Millipore, USA). The microspheres were dried in a vacuum drier for at least 3 days to ensure low moisture content followed by determination of molecular weight.


## 2.6. Determination of Molecular Weight

The molecular weight distribution of Placebo and drug loaded microspheres was determined by gel permeation chromatography (GPC). The GPC system consisted of two Ultrastyragel columns connected in series (7.8 × 300 mm each, one with 104 Å pores and one with 103 Å pores), a delivery device (Shimadzu LC-6A, Japan), UV detector set at λ = 210 nm (Shimadzu, Japan), and software to compute molecular weight distribution (Waters, Maxima 820, Milford, USA). Sample solutions in tetrahydrofuran (THF) at a concentration of 5 mg/mL were filtered through a 0.45 μm filter (Millipore, USA) before injection into the GPC system and were eluted with THF at 0.4 mL/min. The weight-average molecular weight was calculated using monodisperse polystyrene standard where(i)
MWinitial is the initial polymer molecular weight (75 kDa),(ii)
MW0 represents the molecular weight after manufacture of Placebo or drug loaded microspheres (t-zero),(iii)
MWt at time “t=x days” is the molecular weight of a sample (Placebo or drug loaded microspheres).


## 3. Results and Discussion

### 3.1. Drug Content and Particle Size Distribution

Drug content for all the batches of Risperidone and Olanzapine microspheres was assessed by HPLC and the results are described in Table1. Both drugs were easily encapsulated into the PLGA polymer at a range of 1–15% loading for Olanzapine and 1–35% for Risperidone. Thus, at lower loadings, the microsphere formulations had a low drug to polymer ratio, while at higher loadings the drug to polymer ratio was high. Intuitively, this would suggest a greater influence of drug on any drug-polymer interaction at higher loadings.Table 1
Mean particle size of Risperidone and Olanzapine microspheres.
Drug loading (%)
Risperidone microspheres (μm)
Olanzapine microspheres (μm)
1
8.80
10.4
5
12.7
9.80
10
15.4
12.3
15
23.4
10.0
35
20.8
—Results of particle size analysis for Risperidone and Olanzapine microspheres, as measured by laser diffractometry, are listed in Table1. Mean particle size for Olanzapine microspheres remained unchanged, irrespective of drug loading level. In contrast, there is a threefold increase in mean particle size at higher loadings of Risperidone (8.8–23.4 μm). Since the particle size of Risperidone and Olanzapine microspheres at different loadings was small (<25 μm), particle size was not expected to significantly contribute to polymer degradation, especially at particle sizes <15 μm.


### 3.2. Polymer Degradation in Placebo and Drug Loaded Microspheres

In vitro polymer degradation studies were carried out on the Placebo, Risperidone, and Olanzapine microspheres in 0.02 M PBS, pH 7.2 containing 0.05% Tween 80, at 37°C, over 15 days. Using GPC, polymer molecular weight of samples was analyzed at t-zero (MW0), as well as predetermined time-points, for example, t=2,5,10, and 15 days (MWt), for all the microspheres.


#### 3.2.1. Effect of Risperidone on Polymer Degradation

The effect of 1–35% Risperidone loading on degradation of the 65 : 35 PLGA polymer in 0.02 M phospahte buffer saline (PBS) pH 7.2 containing 0.05% Tween 80, at 37°C, is presented as a semilogarithmic plot in Figure2. Unlike the Placebo microspheres where molecular weight was unchanged at t-zero (MWinitial=MW0), with the Risperidone loaded microspheres, a substantial reduction in molecular weight was observed within 2 days (MW2), after which degradation rates slowed considerably. Thus, the degradation profile of the Risperidone microspheres was biphasic. The rapid initial decrease (2 days) in polymer molecular weight was ascribed to an attack from the weakly basic drug to the acidic polymer, followed by a plateau possibly due to neutralization of the weakly basic drug with acids formed during polymer hydration. Thus, after 2 days, the degradation rate for the 1% loaded microspheres was similar to the Placebo microspheres, while the 5% and 10% loading showed only slightly faster degradation rates. At higher loadings of Risperidone, ratio of Risperidone to polymer was high; the loss in molecular weight was ascribed to catalytic effects of the drug on polymer degradation, even though some degree of neutralization of the basic drug was expected. For the 15 and 35% loaded microspheres, the molecular weight reduction after 2 days was the highest (~50%) and the degradation continued to about 80–85% reduction after 15 days. As such, the degradation profiles with the 15 and 35% were quite close, with 15% loading causing slightly greater degradation. These findings suggest that the catalytic effects caused by the tertiary amine groups in Risperidone reach a plateau at high loadings (15–35%).Figure 2
Effect of Risperidone loading on polymer degradation.Results from this study indicate that Risperidone loading had a profound effect on polymer molecular weight reduction at two distinct stages:(a)
after manufacture of the microspheres (MW0<MWinitial),(b)
within 2 days of incubation in PBS (MW2<MW0), followed by less pronounced effects after day 2 of thein vitrodegradation study.


#### 3.2.2. Effect of Olanzapine on Polymer Degradation

The impact of Olanzapine on degradation of the 65 : 35 PLGA polymer in 0.02 M phosphate buffer saline (PBS) pH 7.2 containing 0.05% Tween 80, at 37°C, is shown as a semilogarithmic plot in Figure3. Rapid polymer degradation was observed at all the loadings (1–15%). From Figure 3, it is evident that the degradation rate with Olanzapine followed pseudo first order kinetics, similar to that of the Placebo, albeit much faster. In contrast with Risperidone, where some drug neutralization effects were observed after day 2 with 1% loaded microspheres (lowest loading), the presence of a reactive secondary amine in Olanzapine ensured continual degradation of the PLGA polymer even at the lowest drug load. Notably, the degradation rate for all Olanzapine loaded microspheres was high, and greater than that observed with Risperidone as well as Placebo microspheres. Thus, it is reasonable to assert that, due to the nature and type of chemical functionalities in a molecule such as Olanzapine, it would be difficult to control the degradation of a poly(ester) type polymer like PLGA, even when low amounts of drug levels are incorporated in the microspheres. Overall, the polymer degradation profile observed with Olanzapine microspheres was much faster than that observed for Risperidone. Indeed, at loadings between 5 and 15% Olanzapine, the molecular weight reduction at 15 days was approximately 81–88% of MWinitial.Figure 3
Effect of Olanzapine loading on polymer degradation.Results from this study confirm that, for Olanzapine, substantial molecular weight reduction was noted at two distinct phases:(a)
after manufacture of the microspheres (MW0<MWinitial), similar to Risperidone,(b)
throughout thein vitro degradation study.


#### 3.2.3. Kinetics of Polymer Degradation

As mentioned in the introduction (refer to Section1), PLGA hydrolysis follows pseudo first order kinetics. However, data from Figures 2 and 3 reveal a biphasic degradation profile for the Risperidone microspheres in 0.02 M phosphate buffer saline (PBS) pH 7.2 containing 0.05% Tween 80, at 37°C, but not the Olanzapine and Placebo microspheres. The biphasic degradation profile indicates two varying polymer degradation rates after incorporation of Risperidone into the biodegradable PLGA microsphere (Figure 2). Indeed, a marked difference was observed between the degradation inRegion 1 (0 to 2 days) andRegion 2 (after 2 days till day 15), whereRegion 1 depicts a much faster degradation rate in comparison toRegion 2. This contrasting behavior is ascribed to the effect of Risperidone on the PLGA polymer. Hence, biphasic polymer degradation constants (k1, k2) and half-life (t1/2(1), t1/2(2)) values forRegion 1 andRegion 2 were calculated and are shown in Table 2.Table 2
Rate constant and half-life for Placebo, Olanzapine, and Risperidone microspheres.
% drug loading
Parameter measured
0
1
5
10
15
35
Placebo
Risp.
Olanz.
Risp.
Olanz.
Risp.
Olanz.
Risp.
Olanz.
Risp.
k or k1 (day−1)
0.003
0.148
0.047
0.141
0.077
0.184
0.083
0.359
0.080
0.306
t
1
/
2 or t1/2(1) (day)
213
4.68
14.6
4.91
8.97
3.77
8.35
1.93
8.63
2.267
k
2 (day−1)
—
0.007
—
0.014
—
0.014
—
0.060
—
0.053
t
1
/
2
(
2
) (day)
—
102.3
—
48.1
—
49.5
—
11.5
—
13.2InRegion 1, rate constants for Risperidone appeared relatively constant (between 0.141 and 0.148/day) through 5% loading, after which higher loadings led to higher k1 values, with a plateau in degradation rate at the 15–35% loading range (Table 2). Higher k1 values at higher loadings are not surprising as there is a direct relationship between the increased degradation rate and reduced polymer to drug ratio in the Risperidone microspheres. In contrast, k2 values for Risperidone were significantly lower than k1 values, with a low value of 0.007/day at 1% loading, doubling to 0.014/day for the 5 and 10% loadings to reach a plateau around 0.05-0.06/day at loadings greater than 15% Risperidone (R2>0.95 obtained for k2 at all drug loadings). Given that the sampling time points forRegions 1 and2were predetermined, calculations of rate constants inRegion 1 (Table 2) are reasonable estimates. It should be noted that even if additional data were collected inRegion 1, the overall profile of polymer degradation used to measure the rate constant k1 would not be markedly different than what was observed in Figure 2.Using thek values in Table 2, the half-life values forRegions 1 and2 for both Risperidone loaded microspheres were calculated from the corresponding rate constants. From Table 2, it is evident that half-life values inRegion 1 are faster than those inRegion 2, in some cases by a few orders of magnitude. For Risperidone, t1/2(1) values (1–15% loadings) inRegion 1 ranged from 1.93 to 4.91 days and increased to t1/2(2) values from 11.5 to 102.3 days forRegion 2.In contrast to Risperidone, polymer degradation in Placebo and Olanzapine microspheres followed first order kinetics (Figure3, Table 2). The rate of polymer degradation for the Placebo was low, with a k value of 0.003/day. Incorporation of 1% Olanzapine into the PLGA polymer led to a greater than 10-fold increase in degradation rate (0.047/day) after which the rate nearly doubled to reach 0.077/day with 5% loading and remained relatively constant between 5 and 15% loading. An excellent correlation, R2>0.95, was obtained for the first order rate constant, k, at all drug loadings of Olanzapine. Similar to the trend noted with the rate constant, a half-life value or approximately 14 days was calculated for 1% drug load and dropped to around 8 days for loadings between 5 and 15% of Olanzapine.


#### 3.2.4. Effect of Drug Loading on Polymer Molecular Weight during Manufacture

Unlike Risperidone and Olanzapine, no lowering of polymer molecular weight was observed in the Placebo microsphere during manufacture; there was no difference between MWinitial and MW0 (Figures 2-3). In contrast, Risperidone and Olanzapine had a noticeable influence on the polymer during the manufacturing process. Olanzapine, a secondary amine, being more basic and nucleophilic when compared to Risperidone, caused a greater lowering in polymer molecular weight during manufacture. From an initial molecular weight of 75 kDa (MWinitial), a decrease in polymer molecular weight postmanufacture (MW0) was observed with all the drug loaded microspheres (Figures 2-3). This decrease in polymer molecular weight postmanufacture (MW0) could be fitted to a first order kinetic model (Figure 4).Figure 4
Relationship between drug loading and molecular weight reduction in PLGA microspheres after encapsulation of Risperidone and Olanzapine.A closer inspection of Figure4 reveals that, at 1% loading, MW0 was similar for Olanzapine and Risperidone. However, at higher loadings, incorporation of Olanzapine caused a steeper drop in the molecular weight of PLGA; that is, MW0(Olanzapine)>MW0(Risperidone) (Figure 4). Given that Olanzapine is a stronger base and nucleophile, the reaction with an acidic polymer like PLGA was not unexpected. As such it was noted that, over a 1–15% drug loading range, the value of the slope for Olanzapine was more than three times that observed with Risperidone. The semilogarithmic plot of MW0 values for Risperidone and Olanzapine as a function of loading had a goodness of fit R2>0.9 between 1 and 15% loading for both drugs, indicating that there is a strong correlation between the amount of drug encapsulated in the microspheres and molecular weight loss during manufacture. Though the intercept values are similar, the slopes for Olanzapine and Risperidone seen in Figure 4 are vastly different. These data affirm that the impact of Olanzapine on the degradation of the 65 : 35 PLGA polymer is at least three times higher than Risperidone and were attributed to the catalytic activity of two tertiary amines and a secondary amine in Olanzapine. In comparison, Risperidone does not contain a secondary amine but has two tertiary amines. Since secondary amines are known to be more reactive than tertiary amines, the presence of this functional group in Olanzapine reacted more strongly with the PLGA polymer.Interestingly, a linear relationship was also observed when the % change in molecular weight during manufacture was plotted against % drug loading (Figure5, (2)):
(2)%changeinmol.wt.duringmanufacture=MWinitial-MW0MWinitial×100.Figure 5
Effect of Risperidone and Olanzapine on % change in molecular weight during manufacture.Similar to the plot described in Figure4, the % change in molecular weight with Olanzapine was nearly three times greater than with Risperidone (Figure 5). As such, the % change in molecular weight ranged between 20 and 36% at 1–15% loadings of Risperidone and 20–59% at similar loadings of Olanzapine. The large values of % change in molecular weight observed with Olanzapine suggest that it had a much more debilitating effect on the PLGA polymer. Data analysis of Figure 5 revealed a strong correlation (R2>0.9) between 1 and 15% loading for Risperidone as well as Olanzapine, indicating that there was a good relationship between the amount of drug encapsulated in the microspheres and the % change in molecular weight loss during manufacture.


#### 3.2.5. Extent of Polymer Degradation due to Risperidone and Olanzapine

To gain a better understanding of the extent to which Risperidone and Olanzapine degrade the 65 : 35 PLGA polymer when incubated in 0.02 M phosphate buffer saline pH 7.2 containing 0.05% Tween 80, at 37°C, the molecular weight at day 15 (MW15) was compared with the polymer molecular weight of the microspheres (MW0) (equation (3), Figure 6), as well as the initial polymer molecular weight (MWinitial) (equation (4), Figure 7):
(3)%changeinmol.wt.after15days=MW0-MW15MW0×100.Figure 6
Effect of Risperidone and Olanzapine on % change in molecular weight after 15 days.Figure 7
Extent of polymer degradation due to Risperidone and Olanzapine.Since the molecular weight of the polymer in the microspheres (MW0) varied based on the drug loading (Figures 2-3), the % change in molecular weight that occurred as a result ofin vitro degradation experiments through day 15 was calculated as described in (3) and plotted (Figure 6). At first glance, the high values of % change in molecular weight with Olanzapine are clearly obvious. In fact, even at the low loading of 1% Olanzapine, nearly 50% of the polymer was hydrolyzed through day 15 of thein vitro study. For 5–15% loadings, the % change in molecular weight after 15 days of degradation had reached a plateau between 71 and 73% and was attributed to the similarity in rate constants at higher loadings of Olanzapine (Table 2). On the other hand, a slower drop in molecular weight was noted with Risperidone microspheres. At the low and moderate loadings of 1, 5, and 10%, the % change in molecular weight ranged between 32 and 41%. However, at the highest loading (15%), the % change increased significantly to 78%, slightly more than that noted with the corresponding strength of Olanzapine.Another parameter, that is, the extent of polymer degradation, was calculated to provide an insight to the extent which Risperidone and Olanzapine degrade the polymer. In essence, it is a comparison of the raw polymer molecular weight (MWinitial) against the molecular weight after the 15-day degradation study (MW15) and calculated as described in (4) below
(4)Extentofpolymerdegradation=MWinitial-MW15MWinitial×100.From Figure7, a 61% drop in molecular weight at day 15 was observed with 1% Olanzapine loading, and values reached a plateau at drug loads of 10–15%; that is, extent of polymer degradation was around 86–88%. For Risperidone microspheres, the drop appeared more gradual, increasing from 45% (1% drug load) to 60% (10% drug load) after which a sudden spike in degradation was noted at 15% Risperidone (~86%). A comparison of the data in Figure 7 revealed that, at similar loadings of Risperidone and Olanzapine, the extent of polymer degradation was nearly 15–30% greater with Olanzapine. For instance, at 1% loading of Risperidone, the molecular weight at day 15 (MW15) had dropped to 45% of its initial value (MWinitial), whereas the drop with 1% Olanzapine was much steeper (~61%), that is, a difference of around 15%. At loadings between 5 and 10%, the difference was more marked and ranged around 25–30%; a greater drop in MW15 was observed with Olanzapine. Interestingly, the extent of degradation at 10% loading of Risperidone was quite similar to that observed with 1% loading of Olanzapine. However, the MW15 values for Risperidone and Olanzapine were almost equal (Figures 6-7). Upon closer scrutiny of the data at day 15, it was clear that MW15 values at 10 and 15% Olanzapine had reached a plateau due to extensive breakdown of the polymer.In general, the findings presented in Sections3.2.1 to 3.2.5 of this study are the result of a concerted effort to enable a systematic and efficient formulation development of these molecules in a biodegradable polymer and, consequently, select an appropriate sustained release injectable microsphere dosage form that could provide drug release for 2 or more weeks.The rationale for implementing such a systematic formulation development approach is twofold and offers several advantages.(i) Ensuring That the Right Amount of Drug Loading Is Present in the Delivery Matrix throughout the Intended Duration of Action. The amount of drug loading in a delivery matrix is known to be one of the key modulators of the rate and duration of drug release [29]. For example, dosage forms that contain high drug loading release drug faster due to low polymer content in the dosage form (Figures 2-3). Conversely, a higher polymer to drug ratio should have the opposite effect. For drugs like Risperidone and Olanzapine, using a systematic formulation development strategy would enhance formulation development efforts by: (a) ensuring that sufficient levels of the atypical antipsychotic are present in the delivery matrix and available throughout the duration of actionin vivo[27, 28] and (b) eliminating the need for daily oral dosing and improving patient compliance [6]. Hence, biodegradable microspheres of Risperidone and Olanzapine were evaluated at a range of drug loadings.(ii) Determining the Suitability of the Polymer as a Delivery Matrix. Both Risperidone and Olanzapine are weakly basic nucleophiles that will be encapsulated in a polyester based polymer containing carboxylic end groups [COOH]. Due to the known reactivity of other basic drugs with the PLGA polymer [12, 19], it is critical to understand the impact of Risperidone and Olanzapine on degradation of the 65 : 35 PLGA polymer, as it influences the dosage form performancein vivo.Ensuring that polymer properties like molecular weight are suitable for incorporating a basic drug while retaining sustained delivery characteristics are some important aspects that should be assessed in parallel with dosage form design. This allows for the selection of a suitable polymer for encapsulating moieties like basic drugs that are known to react with poly(ester) polymers like PLA or PLGA. Hence, assessing the impact of varying drug loadings on polymer behavior is a rational approach in the design of polymeric formulations for sustained delivery of atypical antipsychotics and was implemented in this study.As per the formulation rationale presented above, drug loadings between 1 and 15% provided a critical insight about the drug : polymer ratio required for dosage form development. Additionally, since the commercially available Risperidone microspheres contain 38% drug load, a 35% loading of drug for Risperidone was also assessed [30]. Based on these molecular weight results in this study, it would be unwise to neglect the impact of a basic drug on the PLGA polymer during the manufacturing process, thereby reinforcing the importance of understanding chemical properties of drugs and their impact on the polymer.


### 3.3. Effect of pH and Temperature on Polymer Degradation

To obtain a better understanding of the mechanism in which these atypical antipsychotics catalyze polymer hydrolysis, pH and temperature dependency of the degradation process was assessed. In comparing the effect of Risperidone and Olanzapine loadings on polymer degradation, the former has a lower effect on degradation. Hence, for the remainder of the experimental study, Risperidone was chosen as the preferred candidate to evaluate the mechanism by which basic nucleophiles cause degradation of the PLGA polymer and was selected as the model drug for additional studies.


#### 3.3.1. Effect of pH on Degradation of Risperidone PLGA Microspheres

To gain insight into the manner in which Risperidone influences polymer degradation, degradation studies were performed at acidic, neutral, and alkaline pH (pH 5.8, 7.2, and 9.6, resp., at 37°C in 0.02 M PBS containing 0.05% Tween 80) and compared against a control (Placebo microspheres).Figure8 illustrates the effect of pH on the degradation of Placebo and Risperidone microspheres. Since the degradation rate reached a plateau between 15 and 35% loading (Table 2), the higher loading of Risperidone (35%) was selected to ensure greater sensitivity at the 3 pH values used in the degradation study. From the semilogarithmic plot, it is evident that the Placebo microspheres degrade slightly faster under acidic and alkaline pH values but continue to follow pseudo first order kinetics. Interestingly, the profiles at pH 5.8 and 9.6 are almost superimposable, with the extent of degradation (3) around 30% at both conditions, but 2% at neutral pH. As such, the degradation profiles at the acidic and alkaline conditions can be explained using the general acid-base catalysis where ester hydrolysis is enhanced by the presence of H+ or OH− ions in the aqueous media. Thus, the mechanism of PLGA hydrolysis in acidic or alkaline media is different from that observed in neutral media [31].Figure 8
Effect of pH on degradation of Risperidone microspheres at 37°C.When compared to the Placebo microspheres, the presence of Risperidone impacted the reaction profile and increased the rate of degradation of the polymer at all the pH values studied. A biphasic degradation profile was observed with the Risperidone microspheres, similar to that presented in Figure2, at the neutral and alkaline conditions in that a rapid initial decrease in polymer molecular weight was observed at day 2. In contrast, the sharp dip in molecular weight at day 2 was not noted at the acidic pH and was attributed to the neutralization of the weakly basic drug. After day 2, the profiles at the acidic and alkaline pH values were almost superimposable, similar to that observed with the Placebo microspheres. However, the reaction rate slowed down slightly when compared to that at neutral pH, with the extent of degradation (3) around 75% at the acidic and alkaline pH values (~82% at neutral pH). The results at pH values 5.8 and 9.6 implied that degradation of the polymer occurred at a similar rate with the free base and the salt form of Risperidone, indicating that the reaction did not proceed via general acid-base catalysis, but a combination of specific base and/or nucleophilic catalysis.


#### 3.3.2. Effect of Temperature on Degradation of Risperidone Microspheres

The effect of temperature on polymer degradation of Risperidone microspheres was investigated at 0 (Placebo), 1, and 5% loadings at 37°C and 55°C in 0.02 M PBS pH 7.2, and a comparison of the % decrease in polymer molecular weight after 2 days is shown (Figure8). For the Placebo microspheres (0% loading), the rate of polymer degradation increased and followed pseudo first order kinetics. Indeed, the % decrease in molecular weight was well over 30% after just 2 days at 55°C, compared to meager 1% at 37°C. Likewise, the degradation profiles for Risperidone microspheres at 55°C were biphasic, similar to what was observed at 37°C, albeit at a much faster rate (data not shown). After two days of incubation, the extent of reduction in the molecular weight was much higher at 55°C, 46% for the 1% loading, and 76% for the 5% loading. In comparison, the 2-day molecular weight reduction at 37°C was nearly 25% for 1 and 5% Risperidone loadings, indicating that temperature has a pronounced effect on polymer degradation, especially at increased loadings. Overall, polymer degradation was accelerated at the higher temperature with a greater extent in molecular weight reduction observed with increased drug loading (Figure 9).Figure 9
Effect of temperature on the % decrease in molecular weight of Risperidone microspheres after 2 days of incubation at 37 and 55°C.It has been well established that the rate of a chemical reaction is influenced by temperature. With biodegradable polymers like PLGA or PLA, the relationship between temperature and polymer degradation has been explored by a few authors. For example, Buchholz examined the temperature dependency of neat polymer degradation at 37 and 80°C [32]. Another report commented on the polymer degradation profile of neat PLA at a temperature of 90°C [33]. From these studies, it was clear that PLGA and PLA degradation rate was enhanced at higher temperatures and is beneficial to researchers in this area as it allows a substantial reduction in the time and labor needed to assess polymer degradation [34]. Additionally, these studies indicated that increasing the temperature of degradation experiment did not alter the mechanism of polymer degradation. The elevated temperature experiments (accelerated tests) were later extrapolated for their use as a rapid evaluation technique for drug loaded polymeric dosage forms. The success of the devised technique, as noted by the interest in accelerated testing of biodegradable microspheres, is evidenced by a growing number of publications utilizing this methodology [31, 35, 36]. Additionally, accelerated tests have proven to be a useful tool to predict real-timein vitrobehavior of biodegradable microspheres.In the current study, since temperature effects on polymer degradation were expected to be profound, lower loadings of Risperidone microspheres (1 and 5%) were selected for evaluation and the results were compared with a control (Placebo microspheres). The results obtained from Figure9 suggest that the elevated temperatures can be used to accelerate polymer degradation and can be used to evaluate the effect of drug loaded microspheres encapsulated with a basic drug like Risperidone in a rapid manner. These findings are similar to previous reports on neat PLA and PLGA polymers [32–34]. Additionally, no change in the biphasic degradation profile was observed with Risperidone microspheres, further corroborating previous findings from earlier experiments (Figure 2).


## 3.1. Drug Content and Particle Size Distribution

Drug content for all the batches of Risperidone and Olanzapine microspheres was assessed by HPLC and the results are described in Table1. Both drugs were easily encapsulated into the PLGA polymer at a range of 1–15% loading for Olanzapine and 1–35% for Risperidone. Thus, at lower loadings, the microsphere formulations had a low drug to polymer ratio, while at higher loadings the drug to polymer ratio was high. Intuitively, this would suggest a greater influence of drug on any drug-polymer interaction at higher loadings.Table 1
Mean particle size of Risperidone and Olanzapine microspheres.
Drug loading (%)
Risperidone microspheres (μm)
Olanzapine microspheres (μm)
1
8.80
10.4
5
12.7
9.80
10
15.4
12.3
15
23.4
10.0
35
20.8
—Results of particle size analysis for Risperidone and Olanzapine microspheres, as measured by laser diffractometry, are listed in Table1. Mean particle size for Olanzapine microspheres remained unchanged, irrespective of drug loading level. In contrast, there is a threefold increase in mean particle size at higher loadings of Risperidone (8.8–23.4 μm). Since the particle size of Risperidone and Olanzapine microspheres at different loadings was small (<25 μm), particle size was not expected to significantly contribute to polymer degradation, especially at particle sizes <15 μm.


## 3.2. Polymer Degradation in Placebo and Drug Loaded Microspheres

In vitro polymer degradation studies were carried out on the Placebo, Risperidone, and Olanzapine microspheres in 0.02 M PBS, pH 7.2 containing 0.05% Tween 80, at 37°C, over 15 days. Using GPC, polymer molecular weight of samples was analyzed at t-zero (MW0), as well as predetermined time-points, for example, t=2,5,10, and 15 days (MWt), for all the microspheres.


### 3.2.1. Effect of Risperidone on Polymer Degradation

The effect of 1–35% Risperidone loading on degradation of the 65 : 35 PLGA polymer in 0.02 M phospahte buffer saline (PBS) pH 7.2 containing 0.05% Tween 80, at 37°C, is presented as a semilogarithmic plot in Figure2. Unlike the Placebo microspheres where molecular weight was unchanged at t-zero (MWinitial=MW0), with the Risperidone loaded microspheres, a substantial reduction in molecular weight was observed within 2 days (MW2), after which degradation rates slowed considerably. Thus, the degradation profile of the Risperidone microspheres was biphasic. The rapid initial decrease (2 days) in polymer molecular weight was ascribed to an attack from the weakly basic drug to the acidic polymer, followed by a plateau possibly due to neutralization of the weakly basic drug with acids formed during polymer hydration. Thus, after 2 days, the degradation rate for the 1% loaded microspheres was similar to the Placebo microspheres, while the 5% and 10% loading showed only slightly faster degradation rates. At higher loadings of Risperidone, ratio of Risperidone to polymer was high; the loss in molecular weight was ascribed to catalytic effects of the drug on polymer degradation, even though some degree of neutralization of the basic drug was expected. For the 15 and 35% loaded microspheres, the molecular weight reduction after 2 days was the highest (~50%) and the degradation continued to about 80–85% reduction after 15 days. As such, the degradation profiles with the 15 and 35% were quite close, with 15% loading causing slightly greater degradation. These findings suggest that the catalytic effects caused by the tertiary amine groups in Risperidone reach a plateau at high loadings (15–35%).Figure 2
Effect of Risperidone loading on polymer degradation.Results from this study indicate that Risperidone loading had a profound effect on polymer molecular weight reduction at two distinct stages:(a)
after manufacture of the microspheres (MW0<MWinitial),(b)
within 2 days of incubation in PBS (MW2<MW0), followed by less pronounced effects after day 2 of thein vitrodegradation study.


### 3.2.2. Effect of Olanzapine on Polymer Degradation

The impact of Olanzapine on degradation of the 65 : 35 PLGA polymer in 0.02 M phosphate buffer saline (PBS) pH 7.2 containing 0.05% Tween 80, at 37°C, is shown as a semilogarithmic plot in Figure3. Rapid polymer degradation was observed at all the loadings (1–15%). From Figure 3, it is evident that the degradation rate with Olanzapine followed pseudo first order kinetics, similar to that of the Placebo, albeit much faster. In contrast with Risperidone, where some drug neutralization effects were observed after day 2 with 1% loaded microspheres (lowest loading), the presence of a reactive secondary amine in Olanzapine ensured continual degradation of the PLGA polymer even at the lowest drug load. Notably, the degradation rate for all Olanzapine loaded microspheres was high, and greater than that observed with Risperidone as well as Placebo microspheres. Thus, it is reasonable to assert that, due to the nature and type of chemical functionalities in a molecule such as Olanzapine, it would be difficult to control the degradation of a poly(ester) type polymer like PLGA, even when low amounts of drug levels are incorporated in the microspheres. Overall, the polymer degradation profile observed with Olanzapine microspheres was much faster than that observed for Risperidone. Indeed, at loadings between 5 and 15% Olanzapine, the molecular weight reduction at 15 days was approximately 81–88% of MWinitial.Figure 3
Effect of Olanzapine loading on polymer degradation.Results from this study confirm that, for Olanzapine, substantial molecular weight reduction was noted at two distinct phases:(a)
after manufacture of the microspheres (MW0<MWinitial), similar to Risperidone,(b)
throughout thein vitro degradation study.


### 3.2.3. Kinetics of Polymer Degradation

As mentioned in the introduction (refer to Section1), PLGA hydrolysis follows pseudo first order kinetics. However, data from Figures 2 and 3 reveal a biphasic degradation profile for the Risperidone microspheres in 0.02 M phosphate buffer saline (PBS) pH 7.2 containing 0.05% Tween 80, at 37°C, but not the Olanzapine and Placebo microspheres. The biphasic degradation profile indicates two varying polymer degradation rates after incorporation of Risperidone into the biodegradable PLGA microsphere (Figure 2). Indeed, a marked difference was observed between the degradation inRegion 1 (0 to 2 days) andRegion 2 (after 2 days till day 15), whereRegion 1 depicts a much faster degradation rate in comparison toRegion 2. This contrasting behavior is ascribed to the effect of Risperidone on the PLGA polymer. Hence, biphasic polymer degradation constants (k1, k2) and half-life (t1/2(1), t1/2(2)) values forRegion 1 andRegion 2 were calculated and are shown in Table 2.Table 2
Rate constant and half-life for Placebo, Olanzapine, and Risperidone microspheres.
% drug loading
Parameter measured
0
1
5
10
15
35
Placebo
Risp.
Olanz.
Risp.
Olanz.
Risp.
Olanz.
Risp.
Olanz.
Risp.
k or k1 (day−1)
0.003
0.148
0.047
0.141
0.077
0.184
0.083
0.359
0.080
0.306
t
1
/
2 or t1/2(1) (day)
213
4.68
14.6
4.91
8.97
3.77
8.35
1.93
8.63
2.267
k
2 (day−1)
—
0.007
—
0.014
—
0.014
—
0.060
—
0.053
t
1
/
2
(
2
) (day)
—
102.3
—
48.1
—
49.5
—
11.5
—
13.2InRegion 1, rate constants for Risperidone appeared relatively constant (between 0.141 and 0.148/day) through 5% loading, after which higher loadings led to higher k1 values, with a plateau in degradation rate at the 15–35% loading range (Table 2). Higher k1 values at higher loadings are not surprising as there is a direct relationship between the increased degradation rate and reduced polymer to drug ratio in the Risperidone microspheres. In contrast, k2 values for Risperidone were significantly lower than k1 values, with a low value of 0.007/day at 1% loading, doubling to 0.014/day for the 5 and 10% loadings to reach a plateau around 0.05-0.06/day at loadings greater than 15% Risperidone (R2>0.95 obtained for k2 at all drug loadings). Given that the sampling time points forRegions 1 and2were predetermined, calculations of rate constants inRegion 1 (Table 2) are reasonable estimates. It should be noted that even if additional data were collected inRegion 1, the overall profile of polymer degradation used to measure the rate constant k1 would not be markedly different than what was observed in Figure 2.Using thek values in Table 2, the half-life values forRegions 1 and2 for both Risperidone loaded microspheres were calculated from the corresponding rate constants. From Table 2, it is evident that half-life values inRegion 1 are faster than those inRegion 2, in some cases by a few orders of magnitude. For Risperidone, t1/2(1) values (1–15% loadings) inRegion 1 ranged from 1.93 to 4.91 days and increased to t1/2(2) values from 11.5 to 102.3 days forRegion 2.In contrast to Risperidone, polymer degradation in Placebo and Olanzapine microspheres followed first order kinetics (Figure3, Table 2). The rate of polymer degradation for the Placebo was low, with a k value of 0.003/day. Incorporation of 1% Olanzapine into the PLGA polymer led to a greater than 10-fold increase in degradation rate (0.047/day) after which the rate nearly doubled to reach 0.077/day with 5% loading and remained relatively constant between 5 and 15% loading. An excellent correlation, R2>0.95, was obtained for the first order rate constant, k, at all drug loadings of Olanzapine. Similar to the trend noted with the rate constant, a half-life value or approximately 14 days was calculated for 1% drug load and dropped to around 8 days for loadings between 5 and 15% of Olanzapine.


### 3.2.4. Effect of Drug Loading on Polymer Molecular Weight during Manufacture

Unlike Risperidone and Olanzapine, no lowering of polymer molecular weight was observed in the Placebo microsphere during manufacture; there was no difference between MWinitial and MW0 (Figures 2-3). In contrast, Risperidone and Olanzapine had a noticeable influence on the polymer during the manufacturing process. Olanzapine, a secondary amine, being more basic and nucleophilic when compared to Risperidone, caused a greater lowering in polymer molecular weight during manufacture. From an initial molecular weight of 75 kDa (MWinitial), a decrease in polymer molecular weight postmanufacture (MW0) was observed with all the drug loaded microspheres (Figures 2-3). This decrease in polymer molecular weight postmanufacture (MW0) could be fitted to a first order kinetic model (Figure 4).Figure 4
Relationship between drug loading and molecular weight reduction in PLGA microspheres after encapsulation of Risperidone and Olanzapine.A closer inspection of Figure4 reveals that, at 1% loading, MW0 was similar for Olanzapine and Risperidone. However, at higher loadings, incorporation of Olanzapine caused a steeper drop in the molecular weight of PLGA; that is, MW0(Olanzapine)>MW0(Risperidone) (Figure 4). Given that Olanzapine is a stronger base and nucleophile, the reaction with an acidic polymer like PLGA was not unexpected. As such it was noted that, over a 1–15% drug loading range, the value of the slope for Olanzapine was more than three times that observed with Risperidone. The semilogarithmic plot of MW0 values for Risperidone and Olanzapine as a function of loading had a goodness of fit R2>0.9 between 1 and 15% loading for both drugs, indicating that there is a strong correlation between the amount of drug encapsulated in the microspheres and molecular weight loss during manufacture. Though the intercept values are similar, the slopes for Olanzapine and Risperidone seen in Figure 4 are vastly different. These data affirm that the impact of Olanzapine on the degradation of the 65 : 35 PLGA polymer is at least three times higher than Risperidone and were attributed to the catalytic activity of two tertiary amines and a secondary amine in Olanzapine. In comparison, Risperidone does not contain a secondary amine but has two tertiary amines. Since secondary amines are known to be more reactive than tertiary amines, the presence of this functional group in Olanzapine reacted more strongly with the PLGA polymer.Interestingly, a linear relationship was also observed when the % change in molecular weight during manufacture was plotted against % drug loading (Figure5, (2)):
(2)%changeinmol.wt.duringmanufacture=MWinitial-MW0MWinitial×100.Figure 5
Effect of Risperidone and Olanzapine on % change in molecular weight during manufacture.Similar to the plot described in Figure4, the % change in molecular weight with Olanzapine was nearly three times greater than with Risperidone (Figure 5). As such, the % change in molecular weight ranged between 20 and 36% at 1–15% loadings of Risperidone and 20–59% at similar loadings of Olanzapine. The large values of % change in molecular weight observed with Olanzapine suggest that it had a much more debilitating effect on the PLGA polymer. Data analysis of Figure 5 revealed a strong correlation (R2>0.9) between 1 and 15% loading for Risperidone as well as Olanzapine, indicating that there was a good relationship between the amount of drug encapsulated in the microspheres and the % change in molecular weight loss during manufacture.


### 3.2.5. Extent of Polymer Degradation due to Risperidone and Olanzapine

To gain a better understanding of the extent to which Risperidone and Olanzapine degrade the 65 : 35 PLGA polymer when incubated in 0.02 M phosphate buffer saline pH 7.2 containing 0.05% Tween 80, at 37°C, the molecular weight at day 15 (MW15) was compared with the polymer molecular weight of the microspheres (MW0) (equation (3), Figure 6), as well as the initial polymer molecular weight (MWinitial) (equation (4), Figure 7):
(3)%changeinmol.wt.after15days=MW0-MW15MW0×100.Figure 6
Effect of Risperidone and Olanzapine on % change in molecular weight after 15 days.Figure 7
Extent of polymer degradation due to Risperidone and Olanzapine.Since the molecular weight of the polymer in the microspheres (MW0) varied based on the drug loading (Figures 2-3), the % change in molecular weight that occurred as a result ofin vitro degradation experiments through day 15 was calculated as described in (3) and plotted (Figure 6). At first glance, the high values of % change in molecular weight with Olanzapine are clearly obvious. In fact, even at the low loading of 1% Olanzapine, nearly 50% of the polymer was hydrolyzed through day 15 of thein vitro study. For 5–15% loadings, the % change in molecular weight after 15 days of degradation had reached a plateau between 71 and 73% and was attributed to the similarity in rate constants at higher loadings of Olanzapine (Table 2). On the other hand, a slower drop in molecular weight was noted with Risperidone microspheres. At the low and moderate loadings of 1, 5, and 10%, the % change in molecular weight ranged between 32 and 41%. However, at the highest loading (15%), the % change increased significantly to 78%, slightly more than that noted with the corresponding strength of Olanzapine.Another parameter, that is, the extent of polymer degradation, was calculated to provide an insight to the extent which Risperidone and Olanzapine degrade the polymer. In essence, it is a comparison of the raw polymer molecular weight (MWinitial) against the molecular weight after the 15-day degradation study (MW15) and calculated as described in (4) below
(4)Extentofpolymerdegradation=MWinitial-MW15MWinitial×100.From Figure7, a 61% drop in molecular weight at day 15 was observed with 1% Olanzapine loading, and values reached a plateau at drug loads of 10–15%; that is, extent of polymer degradation was around 86–88%. For Risperidone microspheres, the drop appeared more gradual, increasing from 45% (1% drug load) to 60% (10% drug load) after which a sudden spike in degradation was noted at 15% Risperidone (~86%). A comparison of the data in Figure 7 revealed that, at similar loadings of Risperidone and Olanzapine, the extent of polymer degradation was nearly 15–30% greater with Olanzapine. For instance, at 1% loading of Risperidone, the molecular weight at day 15 (MW15) had dropped to 45% of its initial value (MWinitial), whereas the drop with 1% Olanzapine was much steeper (~61%), that is, a difference of around 15%. At loadings between 5 and 10%, the difference was more marked and ranged around 25–30%; a greater drop in MW15 was observed with Olanzapine. Interestingly, the extent of degradation at 10% loading of Risperidone was quite similar to that observed with 1% loading of Olanzapine. However, the MW15 values for Risperidone and Olanzapine were almost equal (Figures 6-7). Upon closer scrutiny of the data at day 15, it was clear that MW15 values at 10 and 15% Olanzapine had reached a plateau due to extensive breakdown of the polymer.In general, the findings presented in Sections3.2.1 to 3.2.5 of this study are the result of a concerted effort to enable a systematic and efficient formulation development of these molecules in a biodegradable polymer and, consequently, select an appropriate sustained release injectable microsphere dosage form that could provide drug release for 2 or more weeks.The rationale for implementing such a systematic formulation development approach is twofold and offers several advantages.(i) Ensuring That the Right Amount of Drug Loading Is Present in the Delivery Matrix throughout the Intended Duration of Action. The amount of drug loading in a delivery matrix is known to be one of the key modulators of the rate and duration of drug release [29]. For example, dosage forms that contain high drug loading release drug faster due to low polymer content in the dosage form (Figures 2-3). Conversely, a higher polymer to drug ratio should have the opposite effect. For drugs like Risperidone and Olanzapine, using a systematic formulation development strategy would enhance formulation development efforts by: (a) ensuring that sufficient levels of the atypical antipsychotic are present in the delivery matrix and available throughout the duration of actionin vivo[27, 28] and (b) eliminating the need for daily oral dosing and improving patient compliance [6]. Hence, biodegradable microspheres of Risperidone and Olanzapine were evaluated at a range of drug loadings.(ii) Determining the Suitability of the Polymer as a Delivery Matrix. Both Risperidone and Olanzapine are weakly basic nucleophiles that will be encapsulated in a polyester based polymer containing carboxylic end groups [COOH]. Due to the known reactivity of other basic drugs with the PLGA polymer [12, 19], it is critical to understand the impact of Risperidone and Olanzapine on degradation of the 65 : 35 PLGA polymer, as it influences the dosage form performancein vivo.Ensuring that polymer properties like molecular weight are suitable for incorporating a basic drug while retaining sustained delivery characteristics are some important aspects that should be assessed in parallel with dosage form design. This allows for the selection of a suitable polymer for encapsulating moieties like basic drugs that are known to react with poly(ester) polymers like PLA or PLGA. Hence, assessing the impact of varying drug loadings on polymer behavior is a rational approach in the design of polymeric formulations for sustained delivery of atypical antipsychotics and was implemented in this study.As per the formulation rationale presented above, drug loadings between 1 and 15% provided a critical insight about the drug : polymer ratio required for dosage form development. Additionally, since the commercially available Risperidone microspheres contain 38% drug load, a 35% loading of drug for Risperidone was also assessed [30]. Based on these molecular weight results in this study, it would be unwise to neglect the impact of a basic drug on the PLGA polymer during the manufacturing process, thereby reinforcing the importance of understanding chemical properties of drugs and their impact on the polymer.


## 3.2.1. Effect of Risperidone on Polymer Degradation

The effect of 1–35% Risperidone loading on degradation of the 65 : 35 PLGA polymer in 0.02 M phospahte buffer saline (PBS) pH 7.2 containing 0.05% Tween 80, at 37°C, is presented as a semilogarithmic plot in Figure2. Unlike the Placebo microspheres where molecular weight was unchanged at t-zero (MWinitial=MW0), with the Risperidone loaded microspheres, a substantial reduction in molecular weight was observed within 2 days (MW2), after which degradation rates slowed considerably. Thus, the degradation profile of the Risperidone microspheres was biphasic. The rapid initial decrease (2 days) in polymer molecular weight was ascribed to an attack from the weakly basic drug to the acidic polymer, followed by a plateau possibly due to neutralization of the weakly basic drug with acids formed during polymer hydration. Thus, after 2 days, the degradation rate for the 1% loaded microspheres was similar to the Placebo microspheres, while the 5% and 10% loading showed only slightly faster degradation rates. At higher loadings of Risperidone, ratio of Risperidone to polymer was high; the loss in molecular weight was ascribed to catalytic effects of the drug on polymer degradation, even though some degree of neutralization of the basic drug was expected. For the 15 and 35% loaded microspheres, the molecular weight reduction after 2 days was the highest (~50%) and the degradation continued to about 80–85% reduction after 15 days. As such, the degradation profiles with the 15 and 35% were quite close, with 15% loading causing slightly greater degradation. These findings suggest that the catalytic effects caused by the tertiary amine groups in Risperidone reach a plateau at high loadings (15–35%).Figure 2
Effect of Risperidone loading on polymer degradation.Results from this study indicate that Risperidone loading had a profound effect on polymer molecular weight reduction at two distinct stages:(a)
after manufacture of the microspheres (MW0<MWinitial),(b)
within 2 days of incubation in PBS (MW2<MW0), followed by less pronounced effects after day 2 of thein vitrodegradation study.


## 3.2.2. Effect of Olanzapine on Polymer Degradation

The impact of Olanzapine on degradation of the 65 : 35 PLGA polymer in 0.02 M phosphate buffer saline (PBS) pH 7.2 containing 0.05% Tween 80, at 37°C, is shown as a semilogarithmic plot in Figure3. Rapid polymer degradation was observed at all the loadings (1–15%). From Figure 3, it is evident that the degradation rate with Olanzapine followed pseudo first order kinetics, similar to that of the Placebo, albeit much faster. In contrast with Risperidone, where some drug neutralization effects were observed after day 2 with 1% loaded microspheres (lowest loading), the presence of a reactive secondary amine in Olanzapine ensured continual degradation of the PLGA polymer even at the lowest drug load. Notably, the degradation rate for all Olanzapine loaded microspheres was high, and greater than that observed with Risperidone as well as Placebo microspheres. Thus, it is reasonable to assert that, due to the nature and type of chemical functionalities in a molecule such as Olanzapine, it would be difficult to control the degradation of a poly(ester) type polymer like PLGA, even when low amounts of drug levels are incorporated in the microspheres. Overall, the polymer degradation profile observed with Olanzapine microspheres was much faster than that observed for Risperidone. Indeed, at loadings between 5 and 15% Olanzapine, the molecular weight reduction at 15 days was approximately 81–88% of MWinitial.Figure 3
Effect of Olanzapine loading on polymer degradation.Results from this study confirm that, for Olanzapine, substantial molecular weight reduction was noted at two distinct phases:(a)
after manufacture of the microspheres (MW0<MWinitial), similar to Risperidone,(b)
throughout thein vitro degradation study.


## 3.2.3. Kinetics of Polymer Degradation

As mentioned in the introduction (refer to Section1), PLGA hydrolysis follows pseudo first order kinetics. However, data from Figures 2 and 3 reveal a biphasic degradation profile for the Risperidone microspheres in 0.02 M phosphate buffer saline (PBS) pH 7.2 containing 0.05% Tween 80, at 37°C, but not the Olanzapine and Placebo microspheres. The biphasic degradation profile indicates two varying polymer degradation rates after incorporation of Risperidone into the biodegradable PLGA microsphere (Figure 2). Indeed, a marked difference was observed between the degradation inRegion 1 (0 to 2 days) andRegion 2 (after 2 days till day 15), whereRegion 1 depicts a much faster degradation rate in comparison toRegion 2. This contrasting behavior is ascribed to the effect of Risperidone on the PLGA polymer. Hence, biphasic polymer degradation constants (k1, k2) and half-life (t1/2(1), t1/2(2)) values forRegion 1 andRegion 2 were calculated and are shown in Table 2.Table 2
Rate constant and half-life for Placebo, Olanzapine, and Risperidone microspheres.
% drug loading
Parameter measured
0
1
5
10
15
35
Placebo
Risp.
Olanz.
Risp.
Olanz.
Risp.
Olanz.
Risp.
Olanz.
Risp.
k or k1 (day−1)
0.003
0.148
0.047
0.141
0.077
0.184
0.083
0.359
0.080
0.306
t
1
/
2 or t1/2(1) (day)
213
4.68
14.6
4.91
8.97
3.77
8.35
1.93
8.63
2.267
k
2 (day−1)
—
0.007
—
0.014
—
0.014
—
0.060
—
0.053
t
1
/
2
(
2
) (day)
—
102.3
—
48.1
—
49.5
—
11.5
—
13.2InRegion 1, rate constants for Risperidone appeared relatively constant (between 0.141 and 0.148/day) through 5% loading, after which higher loadings led to higher k1 values, with a plateau in degradation rate at the 15–35% loading range (Table 2). Higher k1 values at higher loadings are not surprising as there is a direct relationship between the increased degradation rate and reduced polymer to drug ratio in the Risperidone microspheres. In contrast, k2 values for Risperidone were significantly lower than k1 values, with a low value of 0.007/day at 1% loading, doubling to 0.014/day for the 5 and 10% loadings to reach a plateau around 0.05-0.06/day at loadings greater than 15% Risperidone (R2>0.95 obtained for k2 at all drug loadings). Given that the sampling time points forRegions 1 and2were predetermined, calculations of rate constants inRegion 1 (Table 2) are reasonable estimates. It should be noted that even if additional data were collected inRegion 1, the overall profile of polymer degradation used to measure the rate constant k1 would not be markedly different than what was observed in Figure 2.Using thek values in Table 2, the half-life values forRegions 1 and2 for both Risperidone loaded microspheres were calculated from the corresponding rate constants. From Table 2, it is evident that half-life values inRegion 1 are faster than those inRegion 2, in some cases by a few orders of magnitude. For Risperidone, t1/2(1) values (1–15% loadings) inRegion 1 ranged from 1.93 to 4.91 days and increased to t1/2(2) values from 11.5 to 102.3 days forRegion 2.In contrast to Risperidone, polymer degradation in Placebo and Olanzapine microspheres followed first order kinetics (Figure3, Table 2). The rate of polymer degradation for the Placebo was low, with a k value of 0.003/day. Incorporation of 1% Olanzapine into the PLGA polymer led to a greater than 10-fold increase in degradation rate (0.047/day) after which the rate nearly doubled to reach 0.077/day with 5% loading and remained relatively constant between 5 and 15% loading. An excellent correlation, R2>0.95, was obtained for the first order rate constant, k, at all drug loadings of Olanzapine. Similar to the trend noted with the rate constant, a half-life value or approximately 14 days was calculated for 1% drug load and dropped to around 8 days for loadings between 5 and 15% of Olanzapine.


## 3.2.4. Effect of Drug Loading on Polymer Molecular Weight during Manufacture

Unlike Risperidone and Olanzapine, no lowering of polymer molecular weight was observed in the Placebo microsphere during manufacture; there was no difference between MWinitial and MW0 (Figures 2-3). In contrast, Risperidone and Olanzapine had a noticeable influence on the polymer during the manufacturing process. Olanzapine, a secondary amine, being more basic and nucleophilic when compared to Risperidone, caused a greater lowering in polymer molecular weight during manufacture. From an initial molecular weight of 75 kDa (MWinitial), a decrease in polymer molecular weight postmanufacture (MW0) was observed with all the drug loaded microspheres (Figures 2-3). This decrease in polymer molecular weight postmanufacture (MW0) could be fitted to a first order kinetic model (Figure 4).Figure 4
Relationship between drug loading and molecular weight reduction in PLGA microspheres after encapsulation of Risperidone and Olanzapine.A closer inspection of Figure4 reveals that, at 1% loading, MW0 was similar for Olanzapine and Risperidone. However, at higher loadings, incorporation of Olanzapine caused a steeper drop in the molecular weight of PLGA; that is, MW0(Olanzapine)>MW0(Risperidone) (Figure 4). Given that Olanzapine is a stronger base and nucleophile, the reaction with an acidic polymer like PLGA was not unexpected. As such it was noted that, over a 1–15% drug loading range, the value of the slope for Olanzapine was more than three times that observed with Risperidone. The semilogarithmic plot of MW0 values for Risperidone and Olanzapine as a function of loading had a goodness of fit R2>0.9 between 1 and 15% loading for both drugs, indicating that there is a strong correlation between the amount of drug encapsulated in the microspheres and molecular weight loss during manufacture. Though the intercept values are similar, the slopes for Olanzapine and Risperidone seen in Figure 4 are vastly different. These data affirm that the impact of Olanzapine on the degradation of the 65 : 35 PLGA polymer is at least three times higher than Risperidone and were attributed to the catalytic activity of two tertiary amines and a secondary amine in Olanzapine. In comparison, Risperidone does not contain a secondary amine but has two tertiary amines. Since secondary amines are known to be more reactive than tertiary amines, the presence of this functional group in Olanzapine reacted more strongly with the PLGA polymer.Interestingly, a linear relationship was also observed when the % change in molecular weight during manufacture was plotted against % drug loading (Figure5, (2)):
(2)%changeinmol.wt.duringmanufacture=MWinitial-MW0MWinitial×100.Figure 5
Effect of Risperidone and Olanzapine on % change in molecular weight during manufacture.Similar to the plot described in Figure4, the % change in molecular weight with Olanzapine was nearly three times greater than with Risperidone (Figure 5). As such, the % change in molecular weight ranged between 20 and 36% at 1–15% loadings of Risperidone and 20–59% at similar loadings of Olanzapine. The large values of % change in molecular weight observed with Olanzapine suggest that it had a much more debilitating effect on the PLGA polymer. Data analysis of Figure 5 revealed a strong correlation (R2>0.9) between 1 and 15% loading for Risperidone as well as Olanzapine, indicating that there was a good relationship between the amount of drug encapsulated in the microspheres and the % change in molecular weight loss during manufacture.


## 3.2.5. Extent of Polymer Degradation due to Risperidone and Olanzapine

To gain a better understanding of the extent to which Risperidone and Olanzapine degrade the 65 : 35 PLGA polymer when incubated in 0.02 M phosphate buffer saline pH 7.2 containing 0.05% Tween 80, at 37°C, the molecular weight at day 15 (MW15) was compared with the polymer molecular weight of the microspheres (MW0) (equation (3), Figure 6), as well as the initial polymer molecular weight (MWinitial) (equation (4), Figure 7):
(3)%changeinmol.wt.after15days=MW0-MW15MW0×100.Figure 6
Effect of Risperidone and Olanzapine on % change in molecular weight after 15 days.Figure 7
Extent of polymer degradation due to Risperidone and Olanzapine.Since the molecular weight of the polymer in the microspheres (MW0) varied based on the drug loading (Figures 2-3), the % change in molecular weight that occurred as a result ofin vitro degradation experiments through day 15 was calculated as described in (3) and plotted (Figure 6). At first glance, the high values of % change in molecular weight with Olanzapine are clearly obvious. In fact, even at the low loading of 1% Olanzapine, nearly 50% of the polymer was hydrolyzed through day 15 of thein vitro study. For 5–15% loadings, the % change in molecular weight after 15 days of degradation had reached a plateau between 71 and 73% and was attributed to the similarity in rate constants at higher loadings of Olanzapine (Table 2). On the other hand, a slower drop in molecular weight was noted with Risperidone microspheres. At the low and moderate loadings of 1, 5, and 10%, the % change in molecular weight ranged between 32 and 41%. However, at the highest loading (15%), the % change increased significantly to 78%, slightly more than that noted with the corresponding strength of Olanzapine.Another parameter, that is, the extent of polymer degradation, was calculated to provide an insight to the extent which Risperidone and Olanzapine degrade the polymer. In essence, it is a comparison of the raw polymer molecular weight (MWinitial) against the molecular weight after the 15-day degradation study (MW15) and calculated as described in (4) below
(4)Extentofpolymerdegradation=MWinitial-MW15MWinitial×100.From Figure7, a 61% drop in molecular weight at day 15 was observed with 1% Olanzapine loading, and values reached a plateau at drug loads of 10–15%; that is, extent of polymer degradation was around 86–88%. For Risperidone microspheres, the drop appeared more gradual, increasing from 45% (1% drug load) to 60% (10% drug load) after which a sudden spike in degradation was noted at 15% Risperidone (~86%). A comparison of the data in Figure 7 revealed that, at similar loadings of Risperidone and Olanzapine, the extent of polymer degradation was nearly 15–30% greater with Olanzapine. For instance, at 1% loading of Risperidone, the molecular weight at day 15 (MW15) had dropped to 45% of its initial value (MWinitial), whereas the drop with 1% Olanzapine was much steeper (~61%), that is, a difference of around 15%. At loadings between 5 and 10%, the difference was more marked and ranged around 25–30%; a greater drop in MW15 was observed with Olanzapine. Interestingly, the extent of degradation at 10% loading of Risperidone was quite similar to that observed with 1% loading of Olanzapine. However, the MW15 values for Risperidone and Olanzapine were almost equal (Figures 6-7). Upon closer scrutiny of the data at day 15, it was clear that MW15 values at 10 and 15% Olanzapine had reached a plateau due to extensive breakdown of the polymer.In general, the findings presented in Sections3.2.1 to 3.2.5 of this study are the result of a concerted effort to enable a systematic and efficient formulation development of these molecules in a biodegradable polymer and, consequently, select an appropriate sustained release injectable microsphere dosage form that could provide drug release for 2 or more weeks.The rationale for implementing such a systematic formulation development approach is twofold and offers several advantages.(i) Ensuring That the Right Amount of Drug Loading Is Present in the Delivery Matrix throughout the Intended Duration of Action. The amount of drug loading in a delivery matrix is known to be one of the key modulators of the rate and duration of drug release [29]. For example, dosage forms that contain high drug loading release drug faster due to low polymer content in the dosage form (Figures 2-3). Conversely, a higher polymer to drug ratio should have the opposite effect. For drugs like Risperidone and Olanzapine, using a systematic formulation development strategy would enhance formulation development efforts by: (a) ensuring that sufficient levels of the atypical antipsychotic are present in the delivery matrix and available throughout the duration of actionin vivo[27, 28] and (b) eliminating the need for daily oral dosing and improving patient compliance [6]. Hence, biodegradable microspheres of Risperidone and Olanzapine were evaluated at a range of drug loadings.(ii) Determining the Suitability of the Polymer as a Delivery Matrix. Both Risperidone and Olanzapine are weakly basic nucleophiles that will be encapsulated in a polyester based polymer containing carboxylic end groups [COOH]. Due to the known reactivity of other basic drugs with the PLGA polymer [12, 19], it is critical to understand the impact of Risperidone and Olanzapine on degradation of the 65 : 35 PLGA polymer, as it influences the dosage form performancein vivo.Ensuring that polymer properties like molecular weight are suitable for incorporating a basic drug while retaining sustained delivery characteristics are some important aspects that should be assessed in parallel with dosage form design. This allows for the selection of a suitable polymer for encapsulating moieties like basic drugs that are known to react with poly(ester) polymers like PLA or PLGA. Hence, assessing the impact of varying drug loadings on polymer behavior is a rational approach in the design of polymeric formulations for sustained delivery of atypical antipsychotics and was implemented in this study.As per the formulation rationale presented above, drug loadings between 1 and 15% provided a critical insight about the drug : polymer ratio required for dosage form development. Additionally, since the commercially available Risperidone microspheres contain 38% drug load, a 35% loading of drug for Risperidone was also assessed [30]. Based on these molecular weight results in this study, it would be unwise to neglect the impact of a basic drug on the PLGA polymer during the manufacturing process, thereby reinforcing the importance of understanding chemical properties of drugs and their impact on the polymer.


## 3.3. Effect of pH and Temperature on Polymer Degradation

To obtain a better understanding of the mechanism in which these atypical antipsychotics catalyze polymer hydrolysis, pH and temperature dependency of the degradation process was assessed. In comparing the effect of Risperidone and Olanzapine loadings on polymer degradation, the former has a lower effect on degradation. Hence, for the remainder of the experimental study, Risperidone was chosen as the preferred candidate to evaluate the mechanism by which basic nucleophiles cause degradation of the PLGA polymer and was selected as the model drug for additional studies.


### 3.3.1. Effect of pH on Degradation of Risperidone PLGA Microspheres

To gain insight into the manner in which Risperidone influences polymer degradation, degradation studies were performed at acidic, neutral, and alkaline pH (pH 5.8, 7.2, and 9.6, resp., at 37°C in 0.02 M PBS containing 0.05% Tween 80) and compared against a control (Placebo microspheres).Figure8 illustrates the effect of pH on the degradation of Placebo and Risperidone microspheres. Since the degradation rate reached a plateau between 15 and 35% loading (Table 2), the higher loading of Risperidone (35%) was selected to ensure greater sensitivity at the 3 pH values used in the degradation study. From the semilogarithmic plot, it is evident that the Placebo microspheres degrade slightly faster under acidic and alkaline pH values but continue to follow pseudo first order kinetics. Interestingly, the profiles at pH 5.8 and 9.6 are almost superimposable, with the extent of degradation (3) around 30% at both conditions, but 2% at neutral pH. As such, the degradation profiles at the acidic and alkaline conditions can be explained using the general acid-base catalysis where ester hydrolysis is enhanced by the presence of H+ or OH− ions in the aqueous media. Thus, the mechanism of PLGA hydrolysis in acidic or alkaline media is different from that observed in neutral media [31].Figure 8
Effect of pH on degradation of Risperidone microspheres at 37°C.When compared to the Placebo microspheres, the presence of Risperidone impacted the reaction profile and increased the rate of degradation of the polymer at all the pH values studied. A biphasic degradation profile was observed with the Risperidone microspheres, similar to that presented in Figure2, at the neutral and alkaline conditions in that a rapid initial decrease in polymer molecular weight was observed at day 2. In contrast, the sharp dip in molecular weight at day 2 was not noted at the acidic pH and was attributed to the neutralization of the weakly basic drug. After day 2, the profiles at the acidic and alkaline pH values were almost superimposable, similar to that observed with the Placebo microspheres. However, the reaction rate slowed down slightly when compared to that at neutral pH, with the extent of degradation (3) around 75% at the acidic and alkaline pH values (~82% at neutral pH). The results at pH values 5.8 and 9.6 implied that degradation of the polymer occurred at a similar rate with the free base and the salt form of Risperidone, indicating that the reaction did not proceed via general acid-base catalysis, but a combination of specific base and/or nucleophilic catalysis.


### 3.3.2. Effect of Temperature on Degradation of Risperidone Microspheres

The effect of temperature on polymer degradation of Risperidone microspheres was investigated at 0 (Placebo), 1, and 5% loadings at 37°C and 55°C in 0.02 M PBS pH 7.2, and a comparison of the % decrease in polymer molecular weight after 2 days is shown (Figure8). For the Placebo microspheres (0% loading), the rate of polymer degradation increased and followed pseudo first order kinetics. Indeed, the % decrease in molecular weight was well over 30% after just 2 days at 55°C, compared to meager 1% at 37°C. Likewise, the degradation profiles for Risperidone microspheres at 55°C were biphasic, similar to what was observed at 37°C, albeit at a much faster rate (data not shown). After two days of incubation, the extent of reduction in the molecular weight was much higher at 55°C, 46% for the 1% loading, and 76% for the 5% loading. In comparison, the 2-day molecular weight reduction at 37°C was nearly 25% for 1 and 5% Risperidone loadings, indicating that temperature has a pronounced effect on polymer degradation, especially at increased loadings. Overall, polymer degradation was accelerated at the higher temperature with a greater extent in molecular weight reduction observed with increased drug loading (Figure 9).Figure 9
Effect of temperature on the % decrease in molecular weight of Risperidone microspheres after 2 days of incubation at 37 and 55°C.It has been well established that the rate of a chemical reaction is influenced by temperature. With biodegradable polymers like PLGA or PLA, the relationship between temperature and polymer degradation has been explored by a few authors. For example, Buchholz examined the temperature dependency of neat polymer degradation at 37 and 80°C [32]. Another report commented on the polymer degradation profile of neat PLA at a temperature of 90°C [33]. From these studies, it was clear that PLGA and PLA degradation rate was enhanced at higher temperatures and is beneficial to researchers in this area as it allows a substantial reduction in the time and labor needed to assess polymer degradation [34]. Additionally, these studies indicated that increasing the temperature of degradation experiment did not alter the mechanism of polymer degradation. The elevated temperature experiments (accelerated tests) were later extrapolated for their use as a rapid evaluation technique for drug loaded polymeric dosage forms. The success of the devised technique, as noted by the interest in accelerated testing of biodegradable microspheres, is evidenced by a growing number of publications utilizing this methodology [31, 35, 36]. Additionally, accelerated tests have proven to be a useful tool to predict real-timein vitrobehavior of biodegradable microspheres.In the current study, since temperature effects on polymer degradation were expected to be profound, lower loadings of Risperidone microspheres (1 and 5%) were selected for evaluation and the results were compared with a control (Placebo microspheres). The results obtained from Figure9 suggest that the elevated temperatures can be used to accelerate polymer degradation and can be used to evaluate the effect of drug loaded microspheres encapsulated with a basic drug like Risperidone in a rapid manner. These findings are similar to previous reports on neat PLA and PLGA polymers [32–34]. Additionally, no change in the biphasic degradation profile was observed with Risperidone microspheres, further corroborating previous findings from earlier experiments (Figure 2).


## 3.3.1. Effect of pH on Degradation of Risperidone PLGA Microspheres

To gain insight into the manner in which Risperidone influences polymer degradation, degradation studies were performed at acidic, neutral, and alkaline pH (pH 5.8, 7.2, and 9.6, resp., at 37°C in 0.02 M PBS containing 0.05% Tween 80) and compared against a control (Placebo microspheres).Figure8 illustrates the effect of pH on the degradation of Placebo and Risperidone microspheres. Since the degradation rate reached a plateau between 15 and 35% loading (Table 2), the higher loading of Risperidone (35%) was selected to ensure greater sensitivity at the 3 pH values used in the degradation study. From the semilogarithmic plot, it is evident that the Placebo microspheres degrade slightly faster under acidic and alkaline pH values but continue to follow pseudo first order kinetics. Interestingly, the profiles at pH 5.8 and 9.6 are almost superimposable, with the extent of degradation (3) around 30% at both conditions, but 2% at neutral pH. As such, the degradation profiles at the acidic and alkaline conditions can be explained using the general acid-base catalysis where ester hydrolysis is enhanced by the presence of H+ or OH− ions in the aqueous media. Thus, the mechanism of PLGA hydrolysis in acidic or alkaline media is different from that observed in neutral media [31].Figure 8
Effect of pH on degradation of Risperidone microspheres at 37°C.When compared to the Placebo microspheres, the presence of Risperidone impacted the reaction profile and increased the rate of degradation of the polymer at all the pH values studied. A biphasic degradation profile was observed with the Risperidone microspheres, similar to that presented in Figure2, at the neutral and alkaline conditions in that a rapid initial decrease in polymer molecular weight was observed at day 2. In contrast, the sharp dip in molecular weight at day 2 was not noted at the acidic pH and was attributed to the neutralization of the weakly basic drug. After day 2, the profiles at the acidic and alkaline pH values were almost superimposable, similar to that observed with the Placebo microspheres. However, the reaction rate slowed down slightly when compared to that at neutral pH, with the extent of degradation (3) around 75% at the acidic and alkaline pH values (~82% at neutral pH). The results at pH values 5.8 and 9.6 implied that degradation of the polymer occurred at a similar rate with the free base and the salt form of Risperidone, indicating that the reaction did not proceed via general acid-base catalysis, but a combination of specific base and/or nucleophilic catalysis.


## 3.3.2. Effect of Temperature on Degradation of Risperidone Microspheres

The effect of temperature on polymer degradation of Risperidone microspheres was investigated at 0 (Placebo), 1, and 5% loadings at 37°C and 55°C in 0.02 M PBS pH 7.2, and a comparison of the % decrease in polymer molecular weight after 2 days is shown (Figure8). For the Placebo microspheres (0% loading), the rate of polymer degradation increased and followed pseudo first order kinetics. Indeed, the % decrease in molecular weight was well over 30% after just 2 days at 55°C, compared to meager 1% at 37°C. Likewise, the degradation profiles for Risperidone microspheres at 55°C were biphasic, similar to what was observed at 37°C, albeit at a much faster rate (data not shown). After two days of incubation, the extent of reduction in the molecular weight was much higher at 55°C, 46% for the 1% loading, and 76% for the 5% loading. In comparison, the 2-day molecular weight reduction at 37°C was nearly 25% for 1 and 5% Risperidone loadings, indicating that temperature has a pronounced effect on polymer degradation, especially at increased loadings. Overall, polymer degradation was accelerated at the higher temperature with a greater extent in molecular weight reduction observed with increased drug loading (Figure 9).Figure 9
Effect of temperature on the % decrease in molecular weight of Risperidone microspheres after 2 days of incubation at 37 and 55°C.It has been well established that the rate of a chemical reaction is influenced by temperature. With biodegradable polymers like PLGA or PLA, the relationship between temperature and polymer degradation has been explored by a few authors. For example, Buchholz examined the temperature dependency of neat polymer degradation at 37 and 80°C [32]. Another report commented on the polymer degradation profile of neat PLA at a temperature of 90°C [33]. From these studies, it was clear that PLGA and PLA degradation rate was enhanced at higher temperatures and is beneficial to researchers in this area as it allows a substantial reduction in the time and labor needed to assess polymer degradation [34]. Additionally, these studies indicated that increasing the temperature of degradation experiment did not alter the mechanism of polymer degradation. The elevated temperature experiments (accelerated tests) were later extrapolated for their use as a rapid evaluation technique for drug loaded polymeric dosage forms. The success of the devised technique, as noted by the interest in accelerated testing of biodegradable microspheres, is evidenced by a growing number of publications utilizing this methodology [31, 35, 36]. Additionally, accelerated tests have proven to be a useful tool to predict real-timein vitrobehavior of biodegradable microspheres.In the current study, since temperature effects on polymer degradation were expected to be profound, lower loadings of Risperidone microspheres (1 and 5%) were selected for evaluation and the results were compared with a control (Placebo microspheres). The results obtained from Figure9 suggest that the elevated temperatures can be used to accelerate polymer degradation and can be used to evaluate the effect of drug loaded microspheres encapsulated with a basic drug like Risperidone in a rapid manner. These findings are similar to previous reports on neat PLA and PLGA polymers [32–34]. Additionally, no change in the biphasic degradation profile was observed with Risperidone microspheres, further corroborating previous findings from earlier experiments (Figure 2).


## 4. Conclusions

While the biodegradability, biocompatibility, and nonimmunogenicity of PLGA polymers have rendered them suitable for a variety of surgical and medical applications, including dosage forms, the degradative effects of basic and nucleophilic drugs Risperidone and Olanzapine on the polymer diminish their utility with respect to providing sustained levels of encapsulated therapeutic agents over extended intervals. Incorporation of such candidates into PLGA, even at low drug loadings, will greatly enhance polymer degradation and thereby influence formulation performance,in vitro orin vivo. Further, the effect of buffer pH and temperature on polymer molecular weight and degradation should also be considered. All these factors need to be considered while developing PLGA formulations containing basic and nucleophilic drugs for sustained drug delivery.



---

*Source: 154239-2015-01-26.xml*