# Comparing the Yield ofStaphylococcus aureus Recovery with Static versus Agitated Broth Incubation

Authors: Carol E. Muenks; Patrick G. Hogan; Carey-Ann D. Burnham; Stephanie A. Fritz
Journal: Journal of Pathogens
 (2018)

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## Abstract

Given the lack of standardization of methodologies for microbial recovery from built environments, we sought to compare the yield ofStaphylococcus aureus with a broth enrichment method when incubated in agitated versus static conditions. Five unique strains ofS. aureus at five different concentrations were cultured to compare direct plating, agitated broth enrichment, and static broth enrichment culture methods. All samples were incubated at 35° in ambient air. The lowest concentration recovered across three replicates and five strains did not differ between culture methods (Fisher’s exact test, p=0.50); notably, recovery ofS. aureus was equivalent between static and agitated broth incubation. When broth enrichment was used (both static and agitated), the burden ofS. aureus growth was higher (by semiquantitative assessment of 4-quadrant streaking) compared to the direct plating culture method. Optimizing strategies for microbial recovery is essential, particularly in areas of lower biomass, given the paucity of research concerning microbial communities of built environments. The results of this study, in conjunction with other experiments investigating microbiomes of built environments, can help inform protocols for standardizing culturing methods within built environments.


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## Body

## 1. Introduction

There is growing interest in the microbiomes and “resistomes” (the conglomeration of resistance genes) [1] of built environments, such as hospitals, schools, and long-term care facilities [2]. Understanding these microbial communities, their transmission dynamics, and their contribution to person-to-person spread of pathogens could reduce the number of hospital-acquired infections [3]. Standardized methodologies for microbial recovery from the built environment have not been established, making it challenging to compare results between investigations [2]. Current accepted methodologies for cultivatingStaphylococcus aureus in a laboratory setting recommend broth enrichment but are unclear regarding the necessity of agitated incubation [4, 5]. Incubation with agitation requires specialized equipment, which in high volume studies can be cumbersome. The objective of this study was to determine incubation conditions, specifically whether agitated broth incubation is superior to static broth incubation, yielding the highest recovery ofS. aureus.


## 2. Materials and Methods

Using five unique strains ofS. aureus at five specific concentrations, we compared recovery yield with direct plating versus static broth enrichment versus broth enrichment with agitation. Two of the fiveS. aureus strains were reference strains (ATCC 43300: methicillin-resistantS. aureus [MRSA], staphylococcal cassette chromosomemec(SCCmec) Type II and ATCC 25923: methicillin-susceptibleS. aureus [MSSA], SCCmec negative). The remaining three strains ofS. aureus were recovered from community-dwelling individuals (abscess drainage specimen: MRSA, SCCmec type IV [6]; a colonizing anterior nares isolate: MRSA, SCCmec type II [7]; and a colonizing inguinal fold isolate: MSSA, SCCmec type IV [7]). Each strain was suspended in normal saline and prepared to a density of 0.5 McFarland Standard. For each strain, five tenfold dilutions were prepared to achieve concentrations ranging from 0 to 104 colony forming units (CFU)/mL. An ESwab (Becton Dickinson, Franklin Lakes, NJ) was submerged in each of the prepared dilutions, then placed into the ESwab transport medium (eluate), and vortexed for 10 seconds. From the eluate, 100 μL of the eluate was then inoculated onto each of three media: trypticase soy agar with 5% sheep blood (blood agar plate [BAP] BBL; Becton Dickinson) and into two tubes of trypticase soy broth (TSB) with 6.5% NaCl (BBL, BD), which were then vortexed for 10 seconds. The BAP and two TSBs (one static, one agitated at 250 rpm) were incubated in an ambient air incubator at 35°C for 24 hours. Following incubation, 100 μL of each TSB suspension was inoculated onto BAPs using the four quadrant streaking method for semiquantitative analysis [8–10] and incubated for an additional 24 hours. Three independent replications of this experiment were performed. Fisher’s exact test was performed using IBM SPSS for Windows v23 (Chicago, IL) to compare recovery yield between the three culture methods.


## 3. Results

The range of recovery for each of the three culture methods was the same (10-104 CFU/mL). The lowest concentration recovered across three replicates and five strains did not differ between culture methods (Fisher’s exact test, p=0.50); specifically, recovery ofS. aureus was equivalent between static and agitated broth incubation. When broth enrichment was used (both static and agitated), the burden ofS. aureus growth was higher (by semiquantitative assessment of 4-quadrant streaking) compared to the direct plating culture method. Both broth enrichment methods recoveredS. aureus in all four quadrants at 104 CFU/mL, while direct plating only recoveredS. aureus in the first two quadrants at 104 CFU/mL (Table1).Table 1
Recovery ofStaphylococcus aureus by culture method.
S. aureus strain Culture method Replicate, lowest dilution level of detection Lowest dilution that was detected for all replicates, CFU/mL 1 2 3 Reference ATCC 25923MSSA, SCCmec negative Direct Plate 102 102 102 102 Static BE 10 102 103 ≤103 Agitated BE 10 102 103 ≤103 Reference ATCC 43300MRSA, SCCmec type IV Direct Plate 102 102 103 ≤103 Static BE 102 10 102 ≤102 Agitated BE 102 102 103 ≤103 Clinical infecting isolate MRSA, SCCmec Type IV Direct Plate 102 103 102 ≤103 Static BE 102 103 102 ≤103 Agitated BE 102 102 102 102 Clinical colonizing isolate MRSA, SCCmec Type II Direct Plate 103 102 10 ≤103 Static BE 103 102 102 ≤103 Agitated BE 10 102 10 ≤102 Clinical colonizing isolate MSSA, SCCmec Type IV Direct Plate 102 102 102 102 Static BE 10 103 10 ≤103 Agitated BE 10 102 102 ≤102 CFU, colony forming unit; MRSA, methicillin-resistantS. aureus; MSSA, methicillin-susceptible S. aureus;SCCmec,staphylococcal cassette chromosome mec;BE, broth enrichment. Note. S. aureus was recovered at the 104 dilution for all strains and all culture methods at each replicate.The range of recovery for both MRSA and MSSA was 10-104 CFU/mL. The lowest concentration recovered for the two referenceS. aureus strains was 10 CFU/mL, after incubation in static broth. For the two colonizing strains, the lowest concentration recovered was also 10 CFU/mL, occurring six times: once with direct plating, twice with static broth incubation, and three times with agitated broth incubation. The lowest concentration recovered for the infecting isolate was 102 CFU/mL for each of the culture methods.


## 4. Discussion and Conclusions

As interest in the microbial communities of built environments grows, optimal strategies for microbial recovery are essential, particularly in areas of lower biomass. Indeed, the National Academy of Sciences extended a call to close the knowledge gap surrounding how to best investigate the microbiomes of built environments [11]. The present study sought to examine different culturing and incubation methods to best isolateS. aureus from built environments. No significant difference in yield ofS. aureus was observed between static versus agitated broth enrichment, while both broth enrichment methods recovered a higher abundance ofS. aureusthan the direct plating method.The present findings correlate to previous studies which investigated the role of broth enrichment culture methods in recoveringS. aureus. Specifically, Mernelius et al. employed both direct plating and agitated broth enrichment incubation culture methods to elucidate the role of broth enrichment inS. aureus cultivation within throats of newborn infants and found a 46% increase of yield when broth enrichment was used compared to direct plating [12]. Additionally, Tsang et al. used a static broth enrichment culture method to query the potential underestimation ofS. aureuscarriage recovered with standard direct plating techniques, resulting in a 31% increase ofS. aureusyield [13].Although there are limitations to this study including the small sample size and inclusion of only one species (albeit five distinct strains), the insights gathered provide valuable information to the field of study concerning the microbiomes of built environments. This study, in conjunction with the studies outlined above, can contribute to the response to the National Academy of Sciences call [11] to help elucidate protocols for standardized culturing methods within built environments.



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*Source: 1462671-2018-07-26.xml*