# Integrating Traditional Medicine into Modern Inflammatory Diseases Care: Multitargeting byRhus verniciflua Stokes

Authors: Ji Hye Kim; Yong Cheol Shin; Seong-Gyu Ko
Journal: Mediators of Inflammation
 (2014)

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## Abstract

Despite the fact that numerous researches were performed on prevention and treatment of inflammation related diseases, the overall incidence has not changed remarkably. This requires new approaches to overcome inflammation mediated diseases, and thus traditional medicine could be an efficacious source for prevention and treatment of these diseases. In this review, we discuss the contribution of traditional medicine, especiallyRhus verniciflua Stokes, to modern medicine against diverse inflammation mediated diseases. Traditionally, this remedy has been used in Eastern Asia for the treatment of gastric problems, hepatic disorders, infectious diseases, and blood disorders. Modern science has provided the scientific basis for the use of Rhus verniciflua Stokes against such disorders and diseases. Various chemical constituents have been identified from this plant, including phenolic acid, and flavonoids. Cell-based studies have exhibited the potential of this as antibacterial, antioxidant, neuroprotective, anti-inflammatory, growth inhibitory, and anticancer activities. Enormous animal studies have shown the potential of this against proinflammatory diseases, neurodegenerative diseases, diabetes, liver diseases, and chemical insults. At the molecular level, this medicinal plant has been shown to modulate diverse cell-signaling pathways. In clinical studies, Rhus verniciflua Stokes has shown efficacy against various cancer patients such as colorectal, gastric, hepatic, renal, pancreatic, and pulmonary cancers. Thus, this remedy is now exhibiting activities in the clinic.


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## Body

## 1. Introduction

Inflammation is an essential part of the body’s natural responses against harmful stimuli, such as pathogens, toxin, damaged cells, irritants, stress, or injury. Initially, although the symptoms of acute inflammation are unpleasant, they are absolutely necessary for the healing processes. However, sometimes inflammation can cause further inflammation (chronic inflammation), which can last for several months and even years. It can result from failure to eliminate an acute inflammation, an autoimmune response to a self-antigen. Chronic inflammation can eventually cause several diseases and conditions, including some cancers, asthma, rheumatoid arthritis, atherosclerosis, periodontitis, ischemic heart disease, and ulcerative colitis. Therefore, inflammation needs to be well regulated [1].Traditional medicine is a part of traditional East Asian medical systems and has been used for treating various kinds of diseases including cancer for thousands of years, and, recently, increasing emphasis has been focused on the research on traditional medicine. Particularly, many herbs and medicinal plants have been reported to prevent and inhibit various kinds of diseases [2, 3]. Many traditional medicines and their natural products in eastern countries are relatively low priced, are efficacious resources for new drug discovery, and show very little adverse effects identified in clinical research. One of the remedies isToxicodendron vernicifluum,formerlyRhus verniciflua Stokes, which has been used for thousands of years, mostly in Asian countries.Rhus verniciflua Stokes is an Asian tree species of genusToxicodendron, which belongs to Anacardiaceae family, and is cultivated in regions of China, Korea, and Japan [4].Rhus verniciflua Stokes has a long tradition of use in Eastern Asian medical systems. This remedy has been used for enormous purposes since ancient times. In Korea,Rhus verniciflua Stokes has been used as an herbal therapy for the treatment of abdominal masses since the 15th century AD [5]. This was used to relieve stomach problems and liver detoxification and to stop bleeding and cough. It also has been used for digestive problems such as gastritis, helping to break up blood stasis, and purging hardness. It also helps to relieve pain.Rhus verniciflua Stokes has been used as a food additive as well. However, scientific evidence proving these health benefits ofRhus verniciflua Stokes is lacking.In vitro studies of this remedy have shown potential of antibacterial, antimicrobial, antirheumatoid, anti-inflammatory, antioxidant, antigrowth, neuroprotective, antiplatelet aggregation, and anticancer activities (Figure 1). Inin vivo studies, this remedy exhibits activities against inflammatory conditions, neurodegenerative diseases, liver problem, diabetes, arthritis, and atherosclerosis. It has also been shown to protect from numerous chemical insults. Some clinical researches have already evaluated the safety and efficacy ofRhus verniciflua Stokes against cancer patients. In the following sections, we provide the evidence for the biological activities ofRhus verniciflua Stokes from preclinical studies. The common chemical entities isolated fromRhus verniciflua Stokes are also discussed.Figure 1
Schematic representation for the traditional and modern uses ofRhus verniciflua Stokes.


## 2. Preclinical Studies withRhus verniciflua Stokes

Numerous researches from bothin vitro andin vivo studies have indicated the activities ofRhus verniciflua Stokes against numerous diseases. In this section, we provide evidence fromin vitro andin vivo studies for the biological activities ofRhus verniciflua Stokes (Tables 1 and 3).Table 1
Biological activities ofRhus verniciflua Stokes as shown in in vitro studies.
Antibacterial
Exhibited activity againstH. pylori [6].
Anticancer
(i) Exhibited 70% cell death in HeLa and CT-26 tumor cell lines at a minimum concentration of 2.48μM [7].
(ii) Increased DNA fragmentation on the human B and T lymphoma cell lines, BJAB and Jurkat [8].
(iii) Exhibited apoptosisvia caspase-8/PARP cleavage pathway in human osteosarcoma cells [9].
(iv) Exhibited apoptosis induction on SV40-mediated transformed embryonic hepatic cells [10].
(v) Induced apoptosis through an intrinsic pathway in gastric cancer cell lines [11].
(vi) Exhibited caspase-independent death of human osteosarcoma cellsvia p53-mediated mitochondrial stress and nuclear translocation of AIF and endonuclease G [12].
(vii) Enhanced mitochondrial mediated apoptosis by inhibition of the PI3K-Akt/PKB survival pathway in gastric cancer cell lines [13].
(viii) Exhibited potential organ-specific anticancer activity [14].
Antigrowth activity
(i) Inhibited cell proliferation in cultured HeLa and CT-26 tumor cells [7].
(ii) Inhibited the growth of human B, BJAB, and T lymphoma cell lines, Jurkat [8, 15].
(iii) Exhibited sensitive growth inhibition in human osteosarcoma cells [9].
(iv) Exhibited a selective growth inhibition on SV40-mediated transformed embryonic hepatic cells [10].
(v) Exhibited a synergistic inhibitory effect on cell growth in gastric cancer cells at 50μg/mL [11].
(vi) Inhibited the clonogenic growth of small numbers of UACC-812 breast cancer cells cocultured with fibroblastsin vitro [16].
(vii) Suppressed mouse macrophage cell proliferation [17].
Anti-inflammatory
(i) Suppressed proinflammatory mediators NO, PGE2, and TNF-αvia inhibition of NF-κB and JNK pathway in LPS-induced RAW 264.7 macrophages [18].
(ii) Inhibited ROS production and PKC-α translocation, downregulated the expression of NF-κB and AP-1, and inhibited the levels of iNOS and COX-2 expression [19].
(iii) Inhibited the expressions of TNF-α, IL-6, and IL-8 on human mast cells with treatment with PMA and A23187 [20].
(iv) Inhibited LPS-induced NO, PGE2, TNF-α, and IL-1β production via the induction of HO-1 expression in murine macrophages [21].
(v) Suppressed NOSvia the ERK and Akt signaling pathways [22].
(vi) Suppressed iNOS and COX2 mRNA expression induced by LPS and decreased intracellular ROS levels induced by LPS [17].
(vii) Inhibited inflammation-related cytokines and angiogenic factor in rheumatoid arthritic fibroblast-like synovial cells [23].
(viii) Suppressed 2,4-DNFB-induced allergic contact dermatitis [24].
Antioxidative
(i) Exhibited the inhibition of hydroxyl radical-mediated degradation by iron ion chelation [25].
(ii) Exhibited the inhibition of linoleic acid oxidation, protected human LDL from oxidative modification, and protected against plasmid DNA strand breakage induced by peroxyl free radicals [26].
(iii) Exhibited against hydroxyl and peroxyl radicals inin vitro assays [7].
(iv) Inhibited activities of NF-κB and AP-1 induced by G/GO [27].
(v) Reduced intracellular ROS formation caused by H2O2, reduced TBARS formation, and attenuated catalase depletion at concentration of 100 μ/mL [28].
(vi) Prevented cisplatin-induced ROS release against MDCK-I cells [29].
(vii) Protected human keratinocytes against oxidative stress caused by H2O2 [30].
Antiviral
Exhibited antiviral activity against fish pathogenic IHNV and VHSV [31].
Neuroprotection
(i) Protected the murine hippocampal HT22 cells against glutamate-induced neurotoxicity [32].
(ii) Protected dopaminergic neuronal cells in a rotenone model of PD [33].
(iii) Protected against 6-OHDA-induced neuronal cell death of PD [34].
(iv) Protected against rotenone-induced toxicity by preventing the downregulation of BDNF and GDNF in human dopaminergic cells, SH-SY5Y [35, 36].
Other activities
(i) Inhibited platelet aggregationvia inhibition of receptor expression on platelet membranes, including glycoprotein IIb/IIIa (CD41), GPIIb/IIIa-like expression (PAC-1), and P-selectin (CD62), and intracellular calcium mobilization responses and decreased platelet activation were observed for the isomaltol- and pentagalloyl glucose-treated platelets [37].
(ii) Exhibited anti-AKR1B10 activity at 1μM with an IC50 value of 1.47 μM [38].
(iii) Suppressed IL-4 and -10 in BPA-stimulated primary cultured mouse lymphocytes [39].
AIF: apoptosis-inducing factor; AKR1B10: Aldo-keto reductase family 1 B10; AP-1: activator protein-1; BDNF: brain-derived neurotrophic factor; BPA: bisphenol A; COX-2: cyclooxygenase-2; DNA: deoxyribonucleic acid; 2,4-DNFB: 2,4-dinitrofluorobenzene; GDNF: glial cell line-derived neurotrophic factor; G/GO: glucose/glucose oxidase; HO: heme oxygenase;H. pylori: Helicobacter pylori; IC50: the half maximal inhibitory concentration; IHNV: infectious hematopoietic necrosis virus; IL: interleukin; iNOS: inducible nitric oxide synthase; JNK: c-Jun NH(2)-terminal kinase; LDL: low-density lipoprotein; LPS: lipopolysaccharide; NF-κB: nuclear factor kappa B; NO: nitric oxide; NOS: nitric oxide synthase; OHDA: hydroxydopamine; PARP: poly (ADP-ribose) polymerase; PD: Parkinson's disease; PGE2: prostaglandin E2; PI3K: Phosphatidylinositide 3-kinases; PKB: protein kinase B; PKC: protein kinase C; PMA: phorbol 12-myristate 13-acetate; ROS: reactive oxygen species; SV40: Simian virus 40; TBARS: thiobarbituric acid reactive substance; TNF: tumor necrosis factor; VHSV: viral hemorrhagic septicemia virus.


### 2.1. Cell-Based Studies

#### 2.1.1. Antibacterial Activity

Rhus verniciflua Stokes possesses antibacterial activity. In one study, antibacterial activity of the urushiol, major component of the remedy againstHelicobacter pylori (H. pylori), was investigated. All 3 strains,H. pylori NCTC 11637,H. pylori 69, andH. pylori 219, survived within pH 6.0–9.0. The minimal inhibitory concentrations (MIC) of the extract against strains ranged 0.064–0.256 mg/mL [6].


#### 2.1.2. Anticancer Activity

Carcinogenesis is a multistep process involving the transformation, survival, proliferation, angiogenesis, invasion, and metastasis of the tumor and may take over 30 years [40]. Modern science has defined that cancer is a hyperproliferative disorder that involves sustaining proliferative signaling, evading growth suppressors, resisting cell death, inducing angiogenesis, activating invasion and metastasis, and enabling replicative immortality [41]. Extensive research has also demonstrated the biology of cancer. Biological cancer target therapy is those cell signaling pathways, including survival signaling (e.g., phosphatidylinositide 3-kinases- (PI3K-) Akt/protein kinase B (PKB)); cell cycle proteins (e.g., p53, cyclins, and cell cycle dependent kinase inhibitors (CDKIs)); angiogenesis (e.g., vascular endothelial growth factor (VEGF)); and antiapoptosis (e.g., B-cell lymphoma 2 (bcl-2), B-cell lymphoma-extra large (bcl-XL), X-linked inhibitor of apoptosis protein (XIAP), survivin, and FLICE-like inhibitory protein (FLIP)) [2].Rhus verniciflua Stokes has been most widely investigated for its anticancer activity. The most common cancer types in whichRhus verniciflua Stokes has shown potential are those of the liver, blood, breast, bone, and stomach (Table 1). In stomach carcinoma cell model, an ethanol extract ofRhus verniciflua Stokes significantly inhibited G1 cell cycle progressionviap27Kip1 CDKI upregulation and induced mitochondrial apoptosis through the increment of Bax expression, the inhibition of Bcl-2 expression, the release of cytochromec, and the activation of caspase-3 and caspase-9 cascade, and this mechanism byRhus verniciflua Stokes was an enhanced inhibition of the PI3K-Akt/PKB survival pathway [11, 13]. One study concluded that an ethanol extract ofRhus verniciflua Stokes has the potential to induce apoptosis, based on the increase in DNA fragmentation in human B and T lymphoma cell lines, BJAB, and Jurkat [8]. The anticancer activity ofRhus verniciflua Stokes has been shown in human osteosarcoma cells as well. One study investigated the apoptotic effects ofRhus verniciflua Stokes chloroform-methanol fraction from an acetone extract (RCMF) on human osteosarcoma (HOS) cells [9]. PARP cleavage was closely associated with the RCMF-induced apoptosis in HOS cells. Furthermore, the activation of caspase-8 and Bax, the inhibition of Bcl-2 expression, and the release of cytochrome c are shown to be involved in the RCMF-mediated apoptosis. Some other studies using cell line model have also shown the potential ofRhus verniciflua Stokes against various kinds of cancers [14]. Interestingly, among the 4 fractions: diethyl ether, ethyl-acetate (EtOAC), butanol, and water fraction, the EtOAC fraction fromRhus verniciflua Stokes extract contained highly concentrated phenolic compounds, had the most cytotoxic effect in gastric, breast, liver, lung and colon cancer, particularly effective against gastric and breast cancer cells. As well as the EtOAC fraction showed a stronger apoptotic effect on these cells.


#### 2.1.3. Anti-Inflammatory Activity

Chronic inflammation has been associated with numerous human chronic diseases, including cardiovascular, pulmonary, autoimmune, and degenerative diseases, cancer, and diabetes [42]. Many researchers have reported thatRhus verniciflua Stokes possesses an anti-inflammatory effect by modulating the expression of proinflammatory mediators. For example,Rhus verniciflua Stokes exhibited anti-inflammatory activity during endotoxin, lipopolysaccharide (LPS) infection in Raw264.7 macrophage [17–19, 22]. Raw264.7 macrophage is a well-characterized inflammatory model induced by LPS.Rhus verniciflua Stokes showed its activity by suppression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression, which results in inhibiting nitric oxide (NO) and prostaglandin E2 (PGE2) production. All researches studied different kinds of extraction or fractionation methods; for instance, the Jung et al. group used 80% ethanolRhus verniciflua Stokes extract without nonionic compounds, the Oh et al. group used water extract, another Jung group used n-butanol fraction from 80% ethanolRhus verniciflua Stokes extract, and the other group used crude 80% ethanolRhus verniciflua Stokes extract, but the results showed the same effect. Early allergic inflammation is one of the more prominent inflammatory responses and is characterized by the release of histamine and mast cell granule proteins by degranulation, as well as the production of leukotrienes, prostaglandins, and cytokines [43]. One study evaluated the effects ofRhus verniciflua Stokes against phorbol myristate acetate (PMA) and calcium ionophore A23187-induced mast cell activation [20]. The treatment ofRhus verniciflua Stokes significantly modulated the expressions of signal molecules related to allergic inflammatory responsesvia the extracellular signal-regulated kinases (ERK) signaling pathway and inhibited the expressions of inflammation related cytokines—[tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-8] that were stimulated by the treatment with both PMA and A23187.Rhus verniciflua Stokes inhibited the nuclear translocation of nuclear factor- (NF-) κBvia inhibition of the phosphorylation of IkB-α, which are important processes in controlling inflammatory responses as well. Some of the other cancer types in whichRhus verniciflua Stokes has shown anti-inflammatory activities are listed in Table 1. In addition, inflammatory gene products and mechanism regulated byRhus verniciflua Stokes on inflammation are listed in Table 2 specifically.Table 2
Inflammatory gene products and mechanism regulated byRhus verniciflua Stokes.
Model
Inducer
Mechanism (target genes)
[References]
Cell lines
Macrophage
LPS
Inhibited NO, PGE2, and TNF-α production Reduced NF-κB activitySuppressed iNOS and COX-2 protein expression via inactivation of JNK1/2 MAPK kinase pathway
[18]
Macrophage
LPS
Inhibited ROS productionPKC-α translocationDownregulated the expression of NF-κB and AP-1Inhibited the levels of iNOS and COX-2 expression
[19]
Macrophage
LPS
Reduced iNOS at the transcriptional levelDownregulated iNOS protein expression via the ERK and Akt pathway
[22]
HMC-1
PMA, A23187
Inhibited the expressions of TNF-α, IL-6, and IL-8 Suppressed the phosphorylation of ERK and p38 but not JNK Inhibited the nuclear translocation of NF-κB via inhibition of the phosphorylation of IκB-α
[20]
FLS
IL-1β
Decreased TNF-α, IL-6, IL-8, MCP-1, and VEGF Decreased the expression of VEGF via the phosphorylation of p38 MAPK pathway
[23]
Macrophage
LPS
Inhibited NO, PGE2, TNF-α, and IL-1β production via the induction of HO-1 expression
[21]
Animals
Mouse
Carrageenan
Reduced paw edema
[22]
Mouse
Acetic acid
Decreased peritoneal capillary permeability
CMC-Na
Significantly decreased leukocytes migration in peritoneal cavity
Oxazolone
Inhibited ear thickness (DTH)
Collagen
Reduced the incidence and severity of CIA
[21]
Mouse
2,4-DNFB
Reduced ear swelling, hyperplasia of ear tissue
Increased vascular permeability
Decreased numbers of infiltrated mast cells
[24]
AP-1: activator protein-1; CIA: collagen-induced arthritis; COX-2: cyclooxygenase-2; 2,4-DNFB: 2,4-dinitrofluorobenzene; DTH: delayed type hypersensitivity; FLS: rheumatoid arthritic fibroblast-like synovial cells; HMC-1: human mast cells; HO: heme oxygenase; IL: interleukin; iNOS: inducible nitric oxide synthase; JNK: c-Jun NH(2)-terminal kinase; LPS: lipopolysaccharide; MCP-1: monocyte chemoattractant protein; NF-κB: nuclear factor kappa B; NO: nitric oxide; NOS: nitric oxide synthase; oxazolone: 4-ethoxymethylene-2-phenyloxazolone; PGE2: prostaglandin E2; PKC: protein kinase C; PMA: phorbol 12-myristate 13-acetate; ROS: reactive oxygen species; TNF: tumor necrosis factor; VEGF: vascular endothelial growth factor.Table 3
Biological activities ofRhus verniciflua Stokes as shown in in vivo studies.
Model
Effect
Antidiabetic
Rat
Exhibited a decrease in blood glucose levels and blood TBARS concentrations in STZ-induced diabetic rats [44].
Mouse
Decreased in plasma lipid levels (TC, TG, and LDL) and inhibited the activity of HMG-CoA reductase and the levels of TBARS in Triton WR-1339-induced hyperlipidemic mice [45].
Anti-inflammatory
Mouse
Reduced carrageenan-induced mouse paw edema [22].
Mouse
Exhibited activities on vascular permeability, leukocyte migration, and cellular immunity and reduced the incidence and severity of collagen-induced arthritis model [29].
Antioxidative
Mouse
Increased the activities of detoxicant enzymes (CAT, SOD, and GPx) in Triton WR-1339-induced hyperlipidemic mice [45].
Hepatoprotection
Mouse
Suppressed an AFB1-induced increase in serum levels of ALT, ALP, and LDH, prevented MDA formation, and blocked decreases in glutathione levels and SOD [46].
Mouse
Protected from liver damage through inhibited radical scavenging ability, enhanced the activities of antioxidant enzymes, increased the NO production, and decreased the NF-κB and AP-1 activations [47].
Neurodegenerative diseases
Rat
Increased in BDNF and GDNF protein levels in the rat brain [35].
Protection from chemical insults
Mouse
Exhibited potential against cisplatin-induced cytotoxicity and ROS production [29].
Other activities
Mouse
Modulated TPA-induced apoptosis, cytokine production, and T/B cell proliferation in splenocytes [48].
Rat
Exhibited an antifibrogenic activity by inhibition of collagen accumulation and lipid peroxidation and by downregulation of the expression of bothα 1(I) collagen and TIMP-1 mRNA on liver fibrosis induced by CCl4 [49].
AFB-1: aflatoxin B-1; ALP: alkaline phosphatase; ALT: alanine aminotransferase; AP-1: activator protein-1; BDNF: brain-derived neurotrophic factor; CAT: catalase; CCl4: carbon tetrachloride; GDNF: glial cell line-derived neurotrophic factor; GPx: glutathione peroxidase; HMG-CoA: 3-hydroxy-3-methylglutaryl CoA; LDH: lactate dehydrogenase; LDL: low-density lipoprotein; MDA: malondialdehyde; NF-κB: nuclear factor kappa B; NO: nitric oxide; ROS: reactive oxygen species; SOD: superoxide dismutase; STZ: streptozotocin; TBARS: thiobarbituric acid reactive substance; TC: total cholesterol, TG: triglyceride; TIMP-1: tissue inhibitor of metalloproteinases 1; TPA: 12-O-tetradecanoylphorbol 13-acetate.


#### 2.1.4. Growth Inhibitory Effects

Many studies have indicated the growth inhibitory effects ofRhus verniciflua Stokes against numerous cancer cells. For instance,Rhus verniciflua Stokes inhibited the growth of cell proliferation in HeLa (cervical) and CT-26 (colorectal) tumor cells [7]. One study investigated the cytotoxic effects ofRhus verniciflua Stokes in human B, BJAB, and T lymphoma cell lines, Jurkat [8, 15].Rhus verniciflua Stokes was highly cytotoxic to exhibit sensitive growth inhibition in human osteosarcoma cells as well [9]. In another study,Rhus verniciflua Stokes exhibited a selective growth inhibition on SV40-mediated transformed embryonic hepatic cells [10].Rhus verniciflua Stokes showed a synergistic inhibitory effect on cell growth in gastric cancer cells at 50 μg/mL [11]. Another study evaluatedRhus verniciflua Stokes inhibition of the clonogenic growth of small numbers of UACC-812 breast cancer cells cocultured with fibroblastsin vitro[16]. The extract exhibited the potent cytotoxic effects against mouse macrophage cell proliferation [17].


#### 2.1.5. Antioxidant Activity

Rhus verniciflua Stokes acts as a free radical scavenger in a number ofin vitro studies (Table 1). In one study,Rhus verniciflua Stokes exhibited the antioxidant activity in both aqueous and lipidin vitro oxidation reactions using 1,1-diphenyl 2-picrylhydrazyl (DPPH) radical, site-specific Fenton-reaction deoxyribose, and a model lipid emulsion test system against hydroxyl and peroxyl radicals. In the cultured mouse brain, neurons were protected against glucose oxidase-induced hydroxyl radical in the presence of the fractionatedRhus verniciflua Stokes extract (e.g., 58% protection at 4.9 uM) [7]. The antioxidant activity ofRhus verniciflua Stokes is supported by results from otherin vitro assays as well [25, 26]. In macrophage Raw264.7 cell and human keratinocytes model,Rhus verniciflua Stokes prevented the cell cytotoxicity of cells induced by H2O2, respectively, and exhibited antioxidant activities, such as DPPH, superoxide anion, and hydroxyl radical scavenging activities [28, 30].


#### 2.1.6. Antiviral Activity

Rhus verniciflua Stokes has been shown to inhibit the growth of viruses. In one study, the remedy inhibited the growth of fish pathogenic infectious hematopoietic necrosis virus (IHNV) and viral hemorrhagic septicemia virus (VHSV) in flounder spleen (FSP) or chinook salmon embryo- (CHSE-) 214 cells system [31].


#### 2.1.7. Neuroprotection Effects

Additionally, except for the activities discussed above,Rhus verniciflua Stokes exhibited numerous other neuroprotection activities byin vitro studies. For example, in one study,Rhus verniciflua Stokes protected the murine hippocampal HT22 cells against glutamate-induced neurotoxicity [32]. In another study, total extract fromRhus verniciflua Stokes protected dopaminergic neuronal cells in a rotenone model of Parkinson’s disease (PD) [33] and against 6-hydroxydopamine- (OHDA-) induced neuronal cell death of PD. In another study,Rhus verniciflua Stokes protected against rotenone-induced toxicity by preventing the downregulation of brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF) in human dopaminergic cells, SH-SY5Y [35, 36].


#### 2.1.8. Other Activities

In addition to the activities discussed above,Rhus verniciflua Stokes exhibited numerous other activities byin vitro studies. For example, in one study,Rhus verniciflua Stokes showed dose-dependent inhibitory activity towards adenosine diphosphate- (ADP-), collagen-, and arachidonic acid- (AA-) induced aggregation of human platelets [37]. In another study, total extract fromRhus verniciflua Stokes showed Aldo-keto reductase family 1 B10 (AKR1B10), which may be responsible for detoxification of reactive aldehydes, inhibitory activity [38]. Glycoprotein isolated fromRhus verniciflua Stokes (RVS glycoprotein) has an inhibitory activity of T-helper type 2 (Th2) cytokines (IL-4 and -10) in bisphenol A (BPA), one of the estrogen mimic environmental hormones-stimulated primary cultured mouse lymphocytes [39].


### 2.2. Animal-Based Studies

#### 2.2.1. Antidiabetic Activity

Diabetes is a group of metabolic diseases in which a person has high blood sugar, and this disease increases the risk of long-term complications, and, therefore, it should be well regulated like inflammation.Rhus verniciflua Stokes has shown the potential against diabetes in many animal models. For instance, in streptozotocin- (STZ-) induced rat model,Rhus verniciflua Stokes exhibited a decrease in blood glucose levels and blood thiobarbituric acid reactive substance (TBARS) concentrations [44]. Another study examined the modulatory effects ofRhus verniciflua Stokes against hyperlipidemia in WR-1339-induced hyperlipidemic mice model [45]. This remedy decreased plasma lipid levels (total cholesterol (TC), triglyceride (TG), and low-density lipoprotein (LDL)) and inhibited the activity of 3-hydroxy-3-methylglutaryl CoA (HMG-CoA) reductase and the levels of TBARS.


#### 2.2.2. Anti-Inflammatory Effects

Rhus verniciflua Stokes has exhibited the potential against proinflammation in animal models as well. For instance, this remedy reduced carrageenan-induced mouse paw edema [22]. In another study, this extract showed activities on vascular permeability, leukocyte migration, and cellular immunity and reduced the incidence and severity of collagen-induced arthritis model [23].


#### 2.2.3. Antioxidative Effects

In addition to the activities discussed above,Rhus verniciflua Stokes exhibited antioxidative activities byin vivo studies. For example,Rhus verniciflua Stokes increased the activities of detoxicant enzymes (catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx)) in Triton WR-1339-induced hyperlipidemic mice [45].


#### 2.2.4. Hepatoprotection Effects

Rhus verniciflua Stokes has been shown to suppress an aflatoxin B1- (AFB1-) induced increase in serum levels of alanine aminotransferase (ALT), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH), prevent malondialdehyde (MDA) formation, and block decreases in glutathione levels and SOD in mouse model [46]. In another study,Rhus verniciflua Stokes protected from liver damage through inhibition of radical scavenging ability [47].


#### 2.2.5. Protection from Chemical Insults

Rhus verniciflua Stokes has been shown to protect the normal cells, tissues, and organs against the damage caused by external insults. For instance, this remedy exhibited potential against cisplatin-induced cytotoxicity and reactive oxygen species (ROS) production in animal model [29].


#### 2.2.6. Activity against Neurodegenerative Diseases

The most common neurodegenerative disease in whichRhus verniciflua Stokes has shown potential is Parkinson’s disease (PD). Multiple pathways including oxidative stress and mitochondrial damage have been implicated in neurodegeneration during PD. One study evaluated the neuroprotective property ofRhus verniciflua Stokes increased in BDNF and GDNF protein levels, which are critical for the survival and function of developing and adult neurons, learning and memory, and synaptic plasticity, in the rat brain of PD model [35].


#### 2.2.7. Other Activities

In addition to the activities indicated above,Rhus verniciflua Stokes modulated 12-O-tetradecanoylphorbol 13-acetate- (TPA-) induced apoptosis, cytokine production, and T/B cell proliferation in mouse splenocytes [48]. This remedy also exhibited an antifibrogenic activity by inhibition of collagen accumulation and lipid peroxidation and by the downregulation of the expression of both α1(I) collagen and tissue inhibitor of metalloproteinase- (TIMP-) 1 mRNA on liver fibrosis induced by carbon tetrachloride (CCl4) in rat model [49].


## 2.1. Cell-Based Studies

### 2.1.1. Antibacterial Activity

Rhus verniciflua Stokes possesses antibacterial activity. In one study, antibacterial activity of the urushiol, major component of the remedy againstHelicobacter pylori (H. pylori), was investigated. All 3 strains,H. pylori NCTC 11637,H. pylori 69, andH. pylori 219, survived within pH 6.0–9.0. The minimal inhibitory concentrations (MIC) of the extract against strains ranged 0.064–0.256 mg/mL [6].


### 2.1.2. Anticancer Activity

Carcinogenesis is a multistep process involving the transformation, survival, proliferation, angiogenesis, invasion, and metastasis of the tumor and may take over 30 years [40]. Modern science has defined that cancer is a hyperproliferative disorder that involves sustaining proliferative signaling, evading growth suppressors, resisting cell death, inducing angiogenesis, activating invasion and metastasis, and enabling replicative immortality [41]. Extensive research has also demonstrated the biology of cancer. Biological cancer target therapy is those cell signaling pathways, including survival signaling (e.g., phosphatidylinositide 3-kinases- (PI3K-) Akt/protein kinase B (PKB)); cell cycle proteins (e.g., p53, cyclins, and cell cycle dependent kinase inhibitors (CDKIs)); angiogenesis (e.g., vascular endothelial growth factor (VEGF)); and antiapoptosis (e.g., B-cell lymphoma 2 (bcl-2), B-cell lymphoma-extra large (bcl-XL), X-linked inhibitor of apoptosis protein (XIAP), survivin, and FLICE-like inhibitory protein (FLIP)) [2].Rhus verniciflua Stokes has been most widely investigated for its anticancer activity. The most common cancer types in whichRhus verniciflua Stokes has shown potential are those of the liver, blood, breast, bone, and stomach (Table 1). In stomach carcinoma cell model, an ethanol extract ofRhus verniciflua Stokes significantly inhibited G1 cell cycle progressionviap27Kip1 CDKI upregulation and induced mitochondrial apoptosis through the increment of Bax expression, the inhibition of Bcl-2 expression, the release of cytochromec, and the activation of caspase-3 and caspase-9 cascade, and this mechanism byRhus verniciflua Stokes was an enhanced inhibition of the PI3K-Akt/PKB survival pathway [11, 13]. One study concluded that an ethanol extract ofRhus verniciflua Stokes has the potential to induce apoptosis, based on the increase in DNA fragmentation in human B and T lymphoma cell lines, BJAB, and Jurkat [8]. The anticancer activity ofRhus verniciflua Stokes has been shown in human osteosarcoma cells as well. One study investigated the apoptotic effects ofRhus verniciflua Stokes chloroform-methanol fraction from an acetone extract (RCMF) on human osteosarcoma (HOS) cells [9]. PARP cleavage was closely associated with the RCMF-induced apoptosis in HOS cells. Furthermore, the activation of caspase-8 and Bax, the inhibition of Bcl-2 expression, and the release of cytochrome c are shown to be involved in the RCMF-mediated apoptosis. Some other studies using cell line model have also shown the potential ofRhus verniciflua Stokes against various kinds of cancers [14]. Interestingly, among the 4 fractions: diethyl ether, ethyl-acetate (EtOAC), butanol, and water fraction, the EtOAC fraction fromRhus verniciflua Stokes extract contained highly concentrated phenolic compounds, had the most cytotoxic effect in gastric, breast, liver, lung and colon cancer, particularly effective against gastric and breast cancer cells. As well as the EtOAC fraction showed a stronger apoptotic effect on these cells.


### 2.1.3. Anti-Inflammatory Activity

Chronic inflammation has been associated with numerous human chronic diseases, including cardiovascular, pulmonary, autoimmune, and degenerative diseases, cancer, and diabetes [42]. Many researchers have reported thatRhus verniciflua Stokes possesses an anti-inflammatory effect by modulating the expression of proinflammatory mediators. For example,Rhus verniciflua Stokes exhibited anti-inflammatory activity during endotoxin, lipopolysaccharide (LPS) infection in Raw264.7 macrophage [17–19, 22]. Raw264.7 macrophage is a well-characterized inflammatory model induced by LPS.Rhus verniciflua Stokes showed its activity by suppression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression, which results in inhibiting nitric oxide (NO) and prostaglandin E2 (PGE2) production. All researches studied different kinds of extraction or fractionation methods; for instance, the Jung et al. group used 80% ethanolRhus verniciflua Stokes extract without nonionic compounds, the Oh et al. group used water extract, another Jung group used n-butanol fraction from 80% ethanolRhus verniciflua Stokes extract, and the other group used crude 80% ethanolRhus verniciflua Stokes extract, but the results showed the same effect. Early allergic inflammation is one of the more prominent inflammatory responses and is characterized by the release of histamine and mast cell granule proteins by degranulation, as well as the production of leukotrienes, prostaglandins, and cytokines [43]. One study evaluated the effects ofRhus verniciflua Stokes against phorbol myristate acetate (PMA) and calcium ionophore A23187-induced mast cell activation [20]. The treatment ofRhus verniciflua Stokes significantly modulated the expressions of signal molecules related to allergic inflammatory responsesvia the extracellular signal-regulated kinases (ERK) signaling pathway and inhibited the expressions of inflammation related cytokines—[tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-8] that were stimulated by the treatment with both PMA and A23187.Rhus verniciflua Stokes inhibited the nuclear translocation of nuclear factor- (NF-) κBvia inhibition of the phosphorylation of IkB-α, which are important processes in controlling inflammatory responses as well. Some of the other cancer types in whichRhus verniciflua Stokes has shown anti-inflammatory activities are listed in Table 1. In addition, inflammatory gene products and mechanism regulated byRhus verniciflua Stokes on inflammation are listed in Table 2 specifically.Table 2
Inflammatory gene products and mechanism regulated byRhus verniciflua Stokes.
Model
Inducer
Mechanism (target genes)
[References]
Cell lines
Macrophage
LPS
Inhibited NO, PGE2, and TNF-α production Reduced NF-κB activitySuppressed iNOS and COX-2 protein expression via inactivation of JNK1/2 MAPK kinase pathway
[18]
Macrophage
LPS
Inhibited ROS productionPKC-α translocationDownregulated the expression of NF-κB and AP-1Inhibited the levels of iNOS and COX-2 expression
[19]
Macrophage
LPS
Reduced iNOS at the transcriptional levelDownregulated iNOS protein expression via the ERK and Akt pathway
[22]
HMC-1
PMA, A23187
Inhibited the expressions of TNF-α, IL-6, and IL-8 Suppressed the phosphorylation of ERK and p38 but not JNK Inhibited the nuclear translocation of NF-κB via inhibition of the phosphorylation of IκB-α
[20]
FLS
IL-1β
Decreased TNF-α, IL-6, IL-8, MCP-1, and VEGF Decreased the expression of VEGF via the phosphorylation of p38 MAPK pathway
[23]
Macrophage
LPS
Inhibited NO, PGE2, TNF-α, and IL-1β production via the induction of HO-1 expression
[21]
Animals
Mouse
Carrageenan
Reduced paw edema
[22]
Mouse
Acetic acid
Decreased peritoneal capillary permeability
CMC-Na
Significantly decreased leukocytes migration in peritoneal cavity
Oxazolone
Inhibited ear thickness (DTH)
Collagen
Reduced the incidence and severity of CIA
[21]
Mouse
2,4-DNFB
Reduced ear swelling, hyperplasia of ear tissue
Increased vascular permeability
Decreased numbers of infiltrated mast cells
[24]
AP-1: activator protein-1; CIA: collagen-induced arthritis; COX-2: cyclooxygenase-2; 2,4-DNFB: 2,4-dinitrofluorobenzene; DTH: delayed type hypersensitivity; FLS: rheumatoid arthritic fibroblast-like synovial cells; HMC-1: human mast cells; HO: heme oxygenase; IL: interleukin; iNOS: inducible nitric oxide synthase; JNK: c-Jun NH(2)-terminal kinase; LPS: lipopolysaccharide; MCP-1: monocyte chemoattractant protein; NF-κB: nuclear factor kappa B; NO: nitric oxide; NOS: nitric oxide synthase; oxazolone: 4-ethoxymethylene-2-phenyloxazolone; PGE2: prostaglandin E2; PKC: protein kinase C; PMA: phorbol 12-myristate 13-acetate; ROS: reactive oxygen species; TNF: tumor necrosis factor; VEGF: vascular endothelial growth factor.Table 3
Biological activities ofRhus verniciflua Stokes as shown in in vivo studies.
Model
Effect
Antidiabetic
Rat
Exhibited a decrease in blood glucose levels and blood TBARS concentrations in STZ-induced diabetic rats [44].
Mouse
Decreased in plasma lipid levels (TC, TG, and LDL) and inhibited the activity of HMG-CoA reductase and the levels of TBARS in Triton WR-1339-induced hyperlipidemic mice [45].
Anti-inflammatory
Mouse
Reduced carrageenan-induced mouse paw edema [22].
Mouse
Exhibited activities on vascular permeability, leukocyte migration, and cellular immunity and reduced the incidence and severity of collagen-induced arthritis model [29].
Antioxidative
Mouse
Increased the activities of detoxicant enzymes (CAT, SOD, and GPx) in Triton WR-1339-induced hyperlipidemic mice [45].
Hepatoprotection
Mouse
Suppressed an AFB1-induced increase in serum levels of ALT, ALP, and LDH, prevented MDA formation, and blocked decreases in glutathione levels and SOD [46].
Mouse
Protected from liver damage through inhibited radical scavenging ability, enhanced the activities of antioxidant enzymes, increased the NO production, and decreased the NF-κB and AP-1 activations [47].
Neurodegenerative diseases
Rat
Increased in BDNF and GDNF protein levels in the rat brain [35].
Protection from chemical insults
Mouse
Exhibited potential against cisplatin-induced cytotoxicity and ROS production [29].
Other activities
Mouse
Modulated TPA-induced apoptosis, cytokine production, and T/B cell proliferation in splenocytes [48].
Rat
Exhibited an antifibrogenic activity by inhibition of collagen accumulation and lipid peroxidation and by downregulation of the expression of bothα 1(I) collagen and TIMP-1 mRNA on liver fibrosis induced by CCl4 [49].
AFB-1: aflatoxin B-1; ALP: alkaline phosphatase; ALT: alanine aminotransferase; AP-1: activator protein-1; BDNF: brain-derived neurotrophic factor; CAT: catalase; CCl4: carbon tetrachloride; GDNF: glial cell line-derived neurotrophic factor; GPx: glutathione peroxidase; HMG-CoA: 3-hydroxy-3-methylglutaryl CoA; LDH: lactate dehydrogenase; LDL: low-density lipoprotein; MDA: malondialdehyde; NF-κB: nuclear factor kappa B; NO: nitric oxide; ROS: reactive oxygen species; SOD: superoxide dismutase; STZ: streptozotocin; TBARS: thiobarbituric acid reactive substance; TC: total cholesterol, TG: triglyceride; TIMP-1: tissue inhibitor of metalloproteinases 1; TPA: 12-O-tetradecanoylphorbol 13-acetate.


### 2.1.4. Growth Inhibitory Effects

Many studies have indicated the growth inhibitory effects ofRhus verniciflua Stokes against numerous cancer cells. For instance,Rhus verniciflua Stokes inhibited the growth of cell proliferation in HeLa (cervical) and CT-26 (colorectal) tumor cells [7]. One study investigated the cytotoxic effects ofRhus verniciflua Stokes in human B, BJAB, and T lymphoma cell lines, Jurkat [8, 15].Rhus verniciflua Stokes was highly cytotoxic to exhibit sensitive growth inhibition in human osteosarcoma cells as well [9]. In another study,Rhus verniciflua Stokes exhibited a selective growth inhibition on SV40-mediated transformed embryonic hepatic cells [10].Rhus verniciflua Stokes showed a synergistic inhibitory effect on cell growth in gastric cancer cells at 50 μg/mL [11]. Another study evaluatedRhus verniciflua Stokes inhibition of the clonogenic growth of small numbers of UACC-812 breast cancer cells cocultured with fibroblastsin vitro[16]. The extract exhibited the potent cytotoxic effects against mouse macrophage cell proliferation [17].


### 2.1.5. Antioxidant Activity

Rhus verniciflua Stokes acts as a free radical scavenger in a number ofin vitro studies (Table 1). In one study,Rhus verniciflua Stokes exhibited the antioxidant activity in both aqueous and lipidin vitro oxidation reactions using 1,1-diphenyl 2-picrylhydrazyl (DPPH) radical, site-specific Fenton-reaction deoxyribose, and a model lipid emulsion test system against hydroxyl and peroxyl radicals. In the cultured mouse brain, neurons were protected against glucose oxidase-induced hydroxyl radical in the presence of the fractionatedRhus verniciflua Stokes extract (e.g., 58% protection at 4.9 uM) [7]. The antioxidant activity ofRhus verniciflua Stokes is supported by results from otherin vitro assays as well [25, 26]. In macrophage Raw264.7 cell and human keratinocytes model,Rhus verniciflua Stokes prevented the cell cytotoxicity of cells induced by H2O2, respectively, and exhibited antioxidant activities, such as DPPH, superoxide anion, and hydroxyl radical scavenging activities [28, 30].


### 2.1.6. Antiviral Activity

Rhus verniciflua Stokes has been shown to inhibit the growth of viruses. In one study, the remedy inhibited the growth of fish pathogenic infectious hematopoietic necrosis virus (IHNV) and viral hemorrhagic septicemia virus (VHSV) in flounder spleen (FSP) or chinook salmon embryo- (CHSE-) 214 cells system [31].


### 2.1.7. Neuroprotection Effects

Additionally, except for the activities discussed above,Rhus verniciflua Stokes exhibited numerous other neuroprotection activities byin vitro studies. For example, in one study,Rhus verniciflua Stokes protected the murine hippocampal HT22 cells against glutamate-induced neurotoxicity [32]. In another study, total extract fromRhus verniciflua Stokes protected dopaminergic neuronal cells in a rotenone model of Parkinson’s disease (PD) [33] and against 6-hydroxydopamine- (OHDA-) induced neuronal cell death of PD. In another study,Rhus verniciflua Stokes protected against rotenone-induced toxicity by preventing the downregulation of brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF) in human dopaminergic cells, SH-SY5Y [35, 36].


### 2.1.8. Other Activities

In addition to the activities discussed above,Rhus verniciflua Stokes exhibited numerous other activities byin vitro studies. For example, in one study,Rhus verniciflua Stokes showed dose-dependent inhibitory activity towards adenosine diphosphate- (ADP-), collagen-, and arachidonic acid- (AA-) induced aggregation of human platelets [37]. In another study, total extract fromRhus verniciflua Stokes showed Aldo-keto reductase family 1 B10 (AKR1B10), which may be responsible for detoxification of reactive aldehydes, inhibitory activity [38]. Glycoprotein isolated fromRhus verniciflua Stokes (RVS glycoprotein) has an inhibitory activity of T-helper type 2 (Th2) cytokines (IL-4 and -10) in bisphenol A (BPA), one of the estrogen mimic environmental hormones-stimulated primary cultured mouse lymphocytes [39].


## 2.1.1. Antibacterial Activity

Rhus verniciflua Stokes possesses antibacterial activity. In one study, antibacterial activity of the urushiol, major component of the remedy againstHelicobacter pylori (H. pylori), was investigated. All 3 strains,H. pylori NCTC 11637,H. pylori 69, andH. pylori 219, survived within pH 6.0–9.0. The minimal inhibitory concentrations (MIC) of the extract against strains ranged 0.064–0.256 mg/mL [6].


## 2.1.2. Anticancer Activity

Carcinogenesis is a multistep process involving the transformation, survival, proliferation, angiogenesis, invasion, and metastasis of the tumor and may take over 30 years [40]. Modern science has defined that cancer is a hyperproliferative disorder that involves sustaining proliferative signaling, evading growth suppressors, resisting cell death, inducing angiogenesis, activating invasion and metastasis, and enabling replicative immortality [41]. Extensive research has also demonstrated the biology of cancer. Biological cancer target therapy is those cell signaling pathways, including survival signaling (e.g., phosphatidylinositide 3-kinases- (PI3K-) Akt/protein kinase B (PKB)); cell cycle proteins (e.g., p53, cyclins, and cell cycle dependent kinase inhibitors (CDKIs)); angiogenesis (e.g., vascular endothelial growth factor (VEGF)); and antiapoptosis (e.g., B-cell lymphoma 2 (bcl-2), B-cell lymphoma-extra large (bcl-XL), X-linked inhibitor of apoptosis protein (XIAP), survivin, and FLICE-like inhibitory protein (FLIP)) [2].Rhus verniciflua Stokes has been most widely investigated for its anticancer activity. The most common cancer types in whichRhus verniciflua Stokes has shown potential are those of the liver, blood, breast, bone, and stomach (Table 1). In stomach carcinoma cell model, an ethanol extract ofRhus verniciflua Stokes significantly inhibited G1 cell cycle progressionviap27Kip1 CDKI upregulation and induced mitochondrial apoptosis through the increment of Bax expression, the inhibition of Bcl-2 expression, the release of cytochromec, and the activation of caspase-3 and caspase-9 cascade, and this mechanism byRhus verniciflua Stokes was an enhanced inhibition of the PI3K-Akt/PKB survival pathway [11, 13]. One study concluded that an ethanol extract ofRhus verniciflua Stokes has the potential to induce apoptosis, based on the increase in DNA fragmentation in human B and T lymphoma cell lines, BJAB, and Jurkat [8]. The anticancer activity ofRhus verniciflua Stokes has been shown in human osteosarcoma cells as well. One study investigated the apoptotic effects ofRhus verniciflua Stokes chloroform-methanol fraction from an acetone extract (RCMF) on human osteosarcoma (HOS) cells [9]. PARP cleavage was closely associated with the RCMF-induced apoptosis in HOS cells. Furthermore, the activation of caspase-8 and Bax, the inhibition of Bcl-2 expression, and the release of cytochrome c are shown to be involved in the RCMF-mediated apoptosis. Some other studies using cell line model have also shown the potential ofRhus verniciflua Stokes against various kinds of cancers [14]. Interestingly, among the 4 fractions: diethyl ether, ethyl-acetate (EtOAC), butanol, and water fraction, the EtOAC fraction fromRhus verniciflua Stokes extract contained highly concentrated phenolic compounds, had the most cytotoxic effect in gastric, breast, liver, lung and colon cancer, particularly effective against gastric and breast cancer cells. As well as the EtOAC fraction showed a stronger apoptotic effect on these cells.


## 2.1.3. Anti-Inflammatory Activity

Chronic inflammation has been associated with numerous human chronic diseases, including cardiovascular, pulmonary, autoimmune, and degenerative diseases, cancer, and diabetes [42]. Many researchers have reported thatRhus verniciflua Stokes possesses an anti-inflammatory effect by modulating the expression of proinflammatory mediators. For example,Rhus verniciflua Stokes exhibited anti-inflammatory activity during endotoxin, lipopolysaccharide (LPS) infection in Raw264.7 macrophage [17–19, 22]. Raw264.7 macrophage is a well-characterized inflammatory model induced by LPS.Rhus verniciflua Stokes showed its activity by suppression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression, which results in inhibiting nitric oxide (NO) and prostaglandin E2 (PGE2) production. All researches studied different kinds of extraction or fractionation methods; for instance, the Jung et al. group used 80% ethanolRhus verniciflua Stokes extract without nonionic compounds, the Oh et al. group used water extract, another Jung group used n-butanol fraction from 80% ethanolRhus verniciflua Stokes extract, and the other group used crude 80% ethanolRhus verniciflua Stokes extract, but the results showed the same effect. Early allergic inflammation is one of the more prominent inflammatory responses and is characterized by the release of histamine and mast cell granule proteins by degranulation, as well as the production of leukotrienes, prostaglandins, and cytokines [43]. One study evaluated the effects ofRhus verniciflua Stokes against phorbol myristate acetate (PMA) and calcium ionophore A23187-induced mast cell activation [20]. The treatment ofRhus verniciflua Stokes significantly modulated the expressions of signal molecules related to allergic inflammatory responsesvia the extracellular signal-regulated kinases (ERK) signaling pathway and inhibited the expressions of inflammation related cytokines—[tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-8] that were stimulated by the treatment with both PMA and A23187.Rhus verniciflua Stokes inhibited the nuclear translocation of nuclear factor- (NF-) κBvia inhibition of the phosphorylation of IkB-α, which are important processes in controlling inflammatory responses as well. Some of the other cancer types in whichRhus verniciflua Stokes has shown anti-inflammatory activities are listed in Table 1. In addition, inflammatory gene products and mechanism regulated byRhus verniciflua Stokes on inflammation are listed in Table 2 specifically.Table 2
Inflammatory gene products and mechanism regulated byRhus verniciflua Stokes.
Model
Inducer
Mechanism (target genes)
[References]
Cell lines
Macrophage
LPS
Inhibited NO, PGE2, and TNF-α production Reduced NF-κB activitySuppressed iNOS and COX-2 protein expression via inactivation of JNK1/2 MAPK kinase pathway
[18]
Macrophage
LPS
Inhibited ROS productionPKC-α translocationDownregulated the expression of NF-κB and AP-1Inhibited the levels of iNOS and COX-2 expression
[19]
Macrophage
LPS
Reduced iNOS at the transcriptional levelDownregulated iNOS protein expression via the ERK and Akt pathway
[22]
HMC-1
PMA, A23187
Inhibited the expressions of TNF-α, IL-6, and IL-8 Suppressed the phosphorylation of ERK and p38 but not JNK Inhibited the nuclear translocation of NF-κB via inhibition of the phosphorylation of IκB-α
[20]
FLS
IL-1β
Decreased TNF-α, IL-6, IL-8, MCP-1, and VEGF Decreased the expression of VEGF via the phosphorylation of p38 MAPK pathway
[23]
Macrophage
LPS
Inhibited NO, PGE2, TNF-α, and IL-1β production via the induction of HO-1 expression
[21]
Animals
Mouse
Carrageenan
Reduced paw edema
[22]
Mouse
Acetic acid
Decreased peritoneal capillary permeability
CMC-Na
Significantly decreased leukocytes migration in peritoneal cavity
Oxazolone
Inhibited ear thickness (DTH)
Collagen
Reduced the incidence and severity of CIA
[21]
Mouse
2,4-DNFB
Reduced ear swelling, hyperplasia of ear tissue
Increased vascular permeability
Decreased numbers of infiltrated mast cells
[24]
AP-1: activator protein-1; CIA: collagen-induced arthritis; COX-2: cyclooxygenase-2; 2,4-DNFB: 2,4-dinitrofluorobenzene; DTH: delayed type hypersensitivity; FLS: rheumatoid arthritic fibroblast-like synovial cells; HMC-1: human mast cells; HO: heme oxygenase; IL: interleukin; iNOS: inducible nitric oxide synthase; JNK: c-Jun NH(2)-terminal kinase; LPS: lipopolysaccharide; MCP-1: monocyte chemoattractant protein; NF-κB: nuclear factor kappa B; NO: nitric oxide; NOS: nitric oxide synthase; oxazolone: 4-ethoxymethylene-2-phenyloxazolone; PGE2: prostaglandin E2; PKC: protein kinase C; PMA: phorbol 12-myristate 13-acetate; ROS: reactive oxygen species; TNF: tumor necrosis factor; VEGF: vascular endothelial growth factor.Table 3
Biological activities ofRhus verniciflua Stokes as shown in in vivo studies.
Model
Effect
Antidiabetic
Rat
Exhibited a decrease in blood glucose levels and blood TBARS concentrations in STZ-induced diabetic rats [44].
Mouse
Decreased in plasma lipid levels (TC, TG, and LDL) and inhibited the activity of HMG-CoA reductase and the levels of TBARS in Triton WR-1339-induced hyperlipidemic mice [45].
Anti-inflammatory
Mouse
Reduced carrageenan-induced mouse paw edema [22].
Mouse
Exhibited activities on vascular permeability, leukocyte migration, and cellular immunity and reduced the incidence and severity of collagen-induced arthritis model [29].
Antioxidative
Mouse
Increased the activities of detoxicant enzymes (CAT, SOD, and GPx) in Triton WR-1339-induced hyperlipidemic mice [45].
Hepatoprotection
Mouse
Suppressed an AFB1-induced increase in serum levels of ALT, ALP, and LDH, prevented MDA formation, and blocked decreases in glutathione levels and SOD [46].
Mouse
Protected from liver damage through inhibited radical scavenging ability, enhanced the activities of antioxidant enzymes, increased the NO production, and decreased the NF-κB and AP-1 activations [47].
Neurodegenerative diseases
Rat
Increased in BDNF and GDNF protein levels in the rat brain [35].
Protection from chemical insults
Mouse
Exhibited potential against cisplatin-induced cytotoxicity and ROS production [29].
Other activities
Mouse
Modulated TPA-induced apoptosis, cytokine production, and T/B cell proliferation in splenocytes [48].
Rat
Exhibited an antifibrogenic activity by inhibition of collagen accumulation and lipid peroxidation and by downregulation of the expression of bothα 1(I) collagen and TIMP-1 mRNA on liver fibrosis induced by CCl4 [49].
AFB-1: aflatoxin B-1; ALP: alkaline phosphatase; ALT: alanine aminotransferase; AP-1: activator protein-1; BDNF: brain-derived neurotrophic factor; CAT: catalase; CCl4: carbon tetrachloride; GDNF: glial cell line-derived neurotrophic factor; GPx: glutathione peroxidase; HMG-CoA: 3-hydroxy-3-methylglutaryl CoA; LDH: lactate dehydrogenase; LDL: low-density lipoprotein; MDA: malondialdehyde; NF-κB: nuclear factor kappa B; NO: nitric oxide; ROS: reactive oxygen species; SOD: superoxide dismutase; STZ: streptozotocin; TBARS: thiobarbituric acid reactive substance; TC: total cholesterol, TG: triglyceride; TIMP-1: tissue inhibitor of metalloproteinases 1; TPA: 12-O-tetradecanoylphorbol 13-acetate.


## 2.1.4. Growth Inhibitory Effects

Many studies have indicated the growth inhibitory effects ofRhus verniciflua Stokes against numerous cancer cells. For instance,Rhus verniciflua Stokes inhibited the growth of cell proliferation in HeLa (cervical) and CT-26 (colorectal) tumor cells [7]. One study investigated the cytotoxic effects ofRhus verniciflua Stokes in human B, BJAB, and T lymphoma cell lines, Jurkat [8, 15].Rhus verniciflua Stokes was highly cytotoxic to exhibit sensitive growth inhibition in human osteosarcoma cells as well [9]. In another study,Rhus verniciflua Stokes exhibited a selective growth inhibition on SV40-mediated transformed embryonic hepatic cells [10].Rhus verniciflua Stokes showed a synergistic inhibitory effect on cell growth in gastric cancer cells at 50 μg/mL [11]. Another study evaluatedRhus verniciflua Stokes inhibition of the clonogenic growth of small numbers of UACC-812 breast cancer cells cocultured with fibroblastsin vitro[16]. The extract exhibited the potent cytotoxic effects against mouse macrophage cell proliferation [17].


## 2.1.5. Antioxidant Activity

Rhus verniciflua Stokes acts as a free radical scavenger in a number ofin vitro studies (Table 1). In one study,Rhus verniciflua Stokes exhibited the antioxidant activity in both aqueous and lipidin vitro oxidation reactions using 1,1-diphenyl 2-picrylhydrazyl (DPPH) radical, site-specific Fenton-reaction deoxyribose, and a model lipid emulsion test system against hydroxyl and peroxyl radicals. In the cultured mouse brain, neurons were protected against glucose oxidase-induced hydroxyl radical in the presence of the fractionatedRhus verniciflua Stokes extract (e.g., 58% protection at 4.9 uM) [7]. The antioxidant activity ofRhus verniciflua Stokes is supported by results from otherin vitro assays as well [25, 26]. In macrophage Raw264.7 cell and human keratinocytes model,Rhus verniciflua Stokes prevented the cell cytotoxicity of cells induced by H2O2, respectively, and exhibited antioxidant activities, such as DPPH, superoxide anion, and hydroxyl radical scavenging activities [28, 30].


## 2.1.6. Antiviral Activity

Rhus verniciflua Stokes has been shown to inhibit the growth of viruses. In one study, the remedy inhibited the growth of fish pathogenic infectious hematopoietic necrosis virus (IHNV) and viral hemorrhagic septicemia virus (VHSV) in flounder spleen (FSP) or chinook salmon embryo- (CHSE-) 214 cells system [31].


## 2.1.7. Neuroprotection Effects

Additionally, except for the activities discussed above,Rhus verniciflua Stokes exhibited numerous other neuroprotection activities byin vitro studies. For example, in one study,Rhus verniciflua Stokes protected the murine hippocampal HT22 cells against glutamate-induced neurotoxicity [32]. In another study, total extract fromRhus verniciflua Stokes protected dopaminergic neuronal cells in a rotenone model of Parkinson’s disease (PD) [33] and against 6-hydroxydopamine- (OHDA-) induced neuronal cell death of PD. In another study,Rhus verniciflua Stokes protected against rotenone-induced toxicity by preventing the downregulation of brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF) in human dopaminergic cells, SH-SY5Y [35, 36].


## 2.1.8. Other Activities

In addition to the activities discussed above,Rhus verniciflua Stokes exhibited numerous other activities byin vitro studies. For example, in one study,Rhus verniciflua Stokes showed dose-dependent inhibitory activity towards adenosine diphosphate- (ADP-), collagen-, and arachidonic acid- (AA-) induced aggregation of human platelets [37]. In another study, total extract fromRhus verniciflua Stokes showed Aldo-keto reductase family 1 B10 (AKR1B10), which may be responsible for detoxification of reactive aldehydes, inhibitory activity [38]. Glycoprotein isolated fromRhus verniciflua Stokes (RVS glycoprotein) has an inhibitory activity of T-helper type 2 (Th2) cytokines (IL-4 and -10) in bisphenol A (BPA), one of the estrogen mimic environmental hormones-stimulated primary cultured mouse lymphocytes [39].


## 2.2. Animal-Based Studies

### 2.2.1. Antidiabetic Activity

Diabetes is a group of metabolic diseases in which a person has high blood sugar, and this disease increases the risk of long-term complications, and, therefore, it should be well regulated like inflammation.Rhus verniciflua Stokes has shown the potential against diabetes in many animal models. For instance, in streptozotocin- (STZ-) induced rat model,Rhus verniciflua Stokes exhibited a decrease in blood glucose levels and blood thiobarbituric acid reactive substance (TBARS) concentrations [44]. Another study examined the modulatory effects ofRhus verniciflua Stokes against hyperlipidemia in WR-1339-induced hyperlipidemic mice model [45]. This remedy decreased plasma lipid levels (total cholesterol (TC), triglyceride (TG), and low-density lipoprotein (LDL)) and inhibited the activity of 3-hydroxy-3-methylglutaryl CoA (HMG-CoA) reductase and the levels of TBARS.


### 2.2.2. Anti-Inflammatory Effects

Rhus verniciflua Stokes has exhibited the potential against proinflammation in animal models as well. For instance, this remedy reduced carrageenan-induced mouse paw edema [22]. In another study, this extract showed activities on vascular permeability, leukocyte migration, and cellular immunity and reduced the incidence and severity of collagen-induced arthritis model [23].


### 2.2.3. Antioxidative Effects

In addition to the activities discussed above,Rhus verniciflua Stokes exhibited antioxidative activities byin vivo studies. For example,Rhus verniciflua Stokes increased the activities of detoxicant enzymes (catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx)) in Triton WR-1339-induced hyperlipidemic mice [45].


### 2.2.4. Hepatoprotection Effects

Rhus verniciflua Stokes has been shown to suppress an aflatoxin B1- (AFB1-) induced increase in serum levels of alanine aminotransferase (ALT), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH), prevent malondialdehyde (MDA) formation, and block decreases in glutathione levels and SOD in mouse model [46]. In another study,Rhus verniciflua Stokes protected from liver damage through inhibition of radical scavenging ability [47].


### 2.2.5. Protection from Chemical Insults

Rhus verniciflua Stokes has been shown to protect the normal cells, tissues, and organs against the damage caused by external insults. For instance, this remedy exhibited potential against cisplatin-induced cytotoxicity and reactive oxygen species (ROS) production in animal model [29].


### 2.2.6. Activity against Neurodegenerative Diseases

The most common neurodegenerative disease in whichRhus verniciflua Stokes has shown potential is Parkinson’s disease (PD). Multiple pathways including oxidative stress and mitochondrial damage have been implicated in neurodegeneration during PD. One study evaluated the neuroprotective property ofRhus verniciflua Stokes increased in BDNF and GDNF protein levels, which are critical for the survival and function of developing and adult neurons, learning and memory, and synaptic plasticity, in the rat brain of PD model [35].


### 2.2.7. Other Activities

In addition to the activities indicated above,Rhus verniciflua Stokes modulated 12-O-tetradecanoylphorbol 13-acetate- (TPA-) induced apoptosis, cytokine production, and T/B cell proliferation in mouse splenocytes [48]. This remedy also exhibited an antifibrogenic activity by inhibition of collagen accumulation and lipid peroxidation and by the downregulation of the expression of both α1(I) collagen and tissue inhibitor of metalloproteinase- (TIMP-) 1 mRNA on liver fibrosis induced by carbon tetrachloride (CCl4) in rat model [49].


## 2.2.1. Antidiabetic Activity

Diabetes is a group of metabolic diseases in which a person has high blood sugar, and this disease increases the risk of long-term complications, and, therefore, it should be well regulated like inflammation.Rhus verniciflua Stokes has shown the potential against diabetes in many animal models. For instance, in streptozotocin- (STZ-) induced rat model,Rhus verniciflua Stokes exhibited a decrease in blood glucose levels and blood thiobarbituric acid reactive substance (TBARS) concentrations [44]. Another study examined the modulatory effects ofRhus verniciflua Stokes against hyperlipidemia in WR-1339-induced hyperlipidemic mice model [45]. This remedy decreased plasma lipid levels (total cholesterol (TC), triglyceride (TG), and low-density lipoprotein (LDL)) and inhibited the activity of 3-hydroxy-3-methylglutaryl CoA (HMG-CoA) reductase and the levels of TBARS.


## 2.2.2. Anti-Inflammatory Effects

Rhus verniciflua Stokes has exhibited the potential against proinflammation in animal models as well. For instance, this remedy reduced carrageenan-induced mouse paw edema [22]. In another study, this extract showed activities on vascular permeability, leukocyte migration, and cellular immunity and reduced the incidence and severity of collagen-induced arthritis model [23].


## 2.2.3. Antioxidative Effects

In addition to the activities discussed above,Rhus verniciflua Stokes exhibited antioxidative activities byin vivo studies. For example,Rhus verniciflua Stokes increased the activities of detoxicant enzymes (catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx)) in Triton WR-1339-induced hyperlipidemic mice [45].


## 2.2.4. Hepatoprotection Effects

Rhus verniciflua Stokes has been shown to suppress an aflatoxin B1- (AFB1-) induced increase in serum levels of alanine aminotransferase (ALT), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH), prevent malondialdehyde (MDA) formation, and block decreases in glutathione levels and SOD in mouse model [46]. In another study,Rhus verniciflua Stokes protected from liver damage through inhibition of radical scavenging ability [47].


## 2.2.5. Protection from Chemical Insults

Rhus verniciflua Stokes has been shown to protect the normal cells, tissues, and organs against the damage caused by external insults. For instance, this remedy exhibited potential against cisplatin-induced cytotoxicity and reactive oxygen species (ROS) production in animal model [29].


## 2.2.6. Activity against Neurodegenerative Diseases

The most common neurodegenerative disease in whichRhus verniciflua Stokes has shown potential is Parkinson’s disease (PD). Multiple pathways including oxidative stress and mitochondrial damage have been implicated in neurodegeneration during PD. One study evaluated the neuroprotective property ofRhus verniciflua Stokes increased in BDNF and GDNF protein levels, which are critical for the survival and function of developing and adult neurons, learning and memory, and synaptic plasticity, in the rat brain of PD model [35].


## 2.2.7. Other Activities

In addition to the activities indicated above,Rhus verniciflua Stokes modulated 12-O-tetradecanoylphorbol 13-acetate- (TPA-) induced apoptosis, cytokine production, and T/B cell proliferation in mouse splenocytes [48]. This remedy also exhibited an antifibrogenic activity by inhibition of collagen accumulation and lipid peroxidation and by the downregulation of the expression of both α1(I) collagen and tissue inhibitor of metalloproteinase- (TIMP-) 1 mRNA on liver fibrosis induced by carbon tetrachloride (CCl4) in rat model [49].


## 3. Clinical Studies withRhus verniciflua Stokes

Standard modern therapies for cancer treatment include surgery, radiation, chemotherapy, hormone therapy, and palliative care. Cancer surgery still remains the foundation of treatment for cancer patients. However, patients with locally advanced disease such as gastric cancer showed high rates of locoregional or distant recurrence even after potentially curative surgery [104]. Standard chemotherapy and radiation therapy also have clinical limitation in efficacy with severe adverse effects and can act as secondary cancer promoters resulting in chemoresistant cancer cells [105, 106].Rhus verniciflua Stokes has been tested for its potential in human subjects, with about a dozen studies completed to date. Most of these studies have indicated the safety and efficacy ofRhus verniciflua Stokes. The most promising effect ofRhus verniciflua Stokes has been reported against cancer (Table 4). One study demonstrated thatRhus verniciflua Stokes can be administered safely to patients with metastatic colorectal cancer (mCRC) at doses of 450 mg ofRhus verniciflua Stokes that was prescribed [50]. Ten among 36 patients were alive after treatment with 2.7 months (95% confidence interval, 1.9–3.5) median administration period, 10.9 months (95% confidence interval, 5.6–16.1) median overall survival (OS), and 44.4% 1-year survival rate. Although the effects ofRhus verniciflua Stokes continued for several months, hematologic toxicity was not observed and minor adverse effects-mild pruritus and dyspepsia was reported in only 2 of the 36 patients. However, a large scale study is required to further confirm the efficacy and safety ofRhus verniciflua Stokes in mCRC patients and this study has the limitation that 44.4% of patients have chosenRhus verniciflua Stokes as the complementary therapy with conventional treatment including chemotherapy or radiotherapy. In another study,Rhus verniciflua Stokes treatment was well tolerated in advanced pancreatic cancer patients for whom orthodox therapy is unavailable and might prolong overall survival either alone or in combination with chemotherapy [54]. Three out of 42 patients were alive with 3.86 months (95% confidence interval 2.52–5.20) mean administration period, 7.87 months (95% confidence interval 5.14–10.59) median overall survival, and 26.2% 1-year survival rate. Hematologic toxicity related to onlyRhus verniciflua Stokes oral administration was not observed; minor nonhematologic adverse reactions were reported such as mild dyspepsia and pruritus in each patient with toxicity grade 1 pruritus and grade 2 pruritus, respectively. Gemcitabine has emerged as the standard chemotherapy for advanced pancreatic cancer [107]. Many clinical trials have been performed to improve survival by comparing gemcitabine with other agents, either alone or in combination with gemcitabine [108, 109]. In this study, among the patients treated withRhus verniciflua Stokes and concurrent chemotherapy, 19.0% of patients with grade 3 or 4 toxicity were required to discontinue gemcitabine treatment, all patients were not observed a synergistic effect of gemcitabine andRhus vernicifluaStokes compared to alone. AlthoughRhus verniciflua Stokes oral administration was very tolerable, no specific drug interactions betweenRhus verniciflua Stokes and chemotherapy agents were noted, but additional randomized and well-controlled clinical trials with larger number of patients are necessary to confirm its efficacy and safety in the treatment of pancreatic cancer.Table 4
Biological activities ofRhus verniciflua Stokes as shown in clinical studies.
Type
Effect
Anticancer
Colon
Ten among 36 patients were alive after treatment with 2.7 months (95% confidence interval, 1.9–3.5) median administration period, 10.9 months (95% confidence interval, 5.6–16.1) median overall survival, and 44.4% 1-year survival rate [50].
Gastric
Case study: decreased the polypoid mass at the mid body and a slight decrease in the flat elevated lesion at the prepyloric antrum at 5 months after starting daily therapy with 900 mg of orally administered [51].
Liver
Case study: patient with recurrent hepatocellular carcinoma after liver transplantation refractory to doxorubicin exhibited shrinkage of the lung metastasis, nonhematologic toxicity at 5 months after receiving 3 times in a day with 450 mg orally administered [52].
Renal
(i) Case study I: exhibited a complete response in all pulmonary metastases including resolution of right pulmonary artery thrombosis when given at 450 mg capsules with three times a day for 4 months [53].(ii) Case study II: showed reduction in the size of the metastatic masses in both adrenal glands at 9 months after receiving 3 times in a day with 450 mg capsules orally [53].
Pancreatic
Three among 42 patients were alive with 3.86 months (95% confidence interval 2.52–5.20) mean administration period, 7.87 months (95% confidence interval 5.14–10.59) median overall survival, and 26.2% 1-year survival rate [54].
Pulmonary
(i) Case study: maintained good performance status with ECOG performance status of 0 for 2 years after treating daily therapy with 1,350 mg of orally administered remedy without orthodox therapies and no significant adverse effects [55].(ii) Reviewed the medical records of 33 patients with advanced NSCLC, who treated this remedy after completion of four or six cycles of induction chemotherapy for 6 years. A 6- and 12-month PFS rate was 40.6% and 12.9%, respectively. The DCR was 93.9% and the median OS was 34.8 months with 12, 24, and 36 months of overall survival rates which were 84.2%, 76.7%, and 49.9%, respectively. No hematologic toxicity, nephrotoxicity, or hepatotoxicity [56].
DCR: disease control rate; ECOG: European Cooperative Oncology Group; NSCLC: non-small-cell lung carcinoma; OS: overall survival; PFS: progression-free survival.In addition to the clinical research indicated above,Rhus verniciflua Stokes has also been reported to have anticancer activity in various kinds of cancers including gastric, liver, lung, renal, and pulmonary. In the 82-year-old female gastric cancer patient case, orally administeredRhus verniciflua Stokes decreased the polypoid mass at the mid body and a slight decrease in the flat elevated lesion at the prepyloric antrum at 5 months after starting daily therapy with 900 mg [51]. Another 62-year-old Korean male patient with recurrent hepatocellular carcinoma after liver transplantation refractory to doxorubicin exhibited shrinkage of the lung metastasis, nonhematologic toxicity at 5 months after receivingRhus vernicifluaStokes 3 times a day with 450 mg being orally administered [52]. Moreover, two case studies were reported against renal cancer in 2010 as well [53]. Of these, one caseRhus vernicifluaStokes three times in a day with 450 mg capsules for 4 months exhibited a complete response in all pulmonary metastases including resolution of right pulmonary artery thrombosis, and the other case showed reduction in the size of the metastatic masses in both adrenal glands at 9 months after receivingRhus vernicifluaStokes 3 times a day with 450 mg capsules being orally administrated. Another study reported the case of a 52-year-old female who had been diagnosed with pulmonary adenocarcinoma with malignant pleural nodules [55]. The patient maintained good performance status with European Cooperative Oncology Group (ECOG) performance status of 0 for 2 years after daily therapy (1,350 mg of orally administeredRhus vernicifluaStokes remedy without orthodox therapies) and no significant adverse effects. Orally administeredRhus vernicifluaStokes showed an obvious cytostatic effect and could increase the quantity of survival in pulmonary adenocarcinoma; however, further studies with larger populations are required to confirm the claims of the study.


## 4. Main Compounds fromRhus verniciflua Stokes

### 4.1. Chemical Composition ofRhus verniciflua Stokes

Rhus verniciflua Stokes is chemically diverse in composition. To date, around 40 compounds, primarily phenolic acids and flavonoids, have been identified from this remedy (Figure 2) [14, 18, 110]. Of these compounds, 3 are phenolic acid, 4 flavonols, 4 flavanonols, 3 flavones, 1 chalconoid, and 2 tannins. The most common constituents present inRhus verniciflua Stokes are butein, which is a chalconoid having antibacterial, antifungal, antitumor, and anti-inflammatory properties, quercetin, which is a flavonol having antiviral, antiasthma, anticancer, antiprostatitis, and anti-inflammatory properties, and sulfuretin, which is a flavanonol possessing antioxidative, antidiabetes, anticancer, antiviral, and anti-inflammatory properties. The chemical structure and high-pressure liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS) analysis of some other compounds identified fromRhus verniciflua Stokes are shown in Figures 2 and 3 [14, 18].Figure 2
Molecular structure of common constituents ofRhus verniciflua Stokes.High-performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS) analysis of constituents identified fromRhus verniciflua Stokes. (a) HPLC chromatogram of standard compounds (upper) and purifiedRhus verniciflua Stokes extract (lower) at 280 nm and 360 nm: 1: protocatechuic acid, 2:p-hydroxybenzoic acid, 3: caffeic acid, 4: chlorogenic acid, 5:p-coumaric acid, 6: phloretin-2-O-glucoside, 7: fustin, 8: kaempferol-3-O-glucoside, 9: sulfuretin, 10: quercetin, 11: butein, and 12: kaempferol. (b) LC-MS chromatogram of phenolic-rich EtOAC fraction fromRhus verniciflua Stokes extract: 1: gallic acid, 2: protocatechuic acid, 3–9: 8 unknown compounds, 11: fisetin, 12: sulfuretin, and 13: butein.
(a)
(b)


### 4.2. Biological Activities of Main Compounds fromRhus verniciflua Stokes

Many researches have been studied to identify the clinically active ingredient fromRhus verniciflua Stokes like aspirin which was first discovered from the bark of the willow tree in 1763 by Edward Stone of Wadham College, Oxford University. Recently flavonoids fromRhus verniciflua Stokes have been found to have various biological activities, including antiproliferative, anti-inflammatory, and apoptotic activities in human cancer cell lines andin vivo model. For example, butein fromRhus verniciflua Stokes inhibited clonogenic growth of human breast cancer cells cocultured with fibroblasts [16], human colon adenocarcinoma cell proliferation [111], and prostate tumor growthin vitro andin vivo [59]. Moreover, this chalcone exhibited inhibition of NF-κB activation and infiltration reduction of inflammatory cells and apoptosis after spinal cord injury in animal model [60]. In another study, this tetrahydroxychalcone protected the murine hippocampal HT22 cells against glutamate-induced neurotoxicity, attenuated reactive oxygen species (ROS) generations through preserving the activities of SOD, GR, and GSH-Px, showed inhibitory effects on LPS-induced NO production, and suppressed the expression of both iNOS and COX-2 in BV2 cells [32]. Other main compounds, kaempferol, quercetin, and fisetin, have similar biological activities, including antiproliferative, anti-inflammatory, antioxidative, and apoptotic activities in human cancer cell lines andin vivo model. Their activities could come from a similar structure base. Some of the other constituents in whichRhus verniciflua Stokes has shown biological activities are listed in Table 5.Table 5
Selected biological activities of selected main compounds fromRhus verniciflua Stokes.
Type
Effect
Butein
(i) Exhibited aldose reductase and advanced glycation end-products inhibition [57].(ii) Protected pancreatic beta cells (INS-1 cells) against cytokine-induced toxicity mediated by inhibition of NO formation at concentrations of 15–30 μM [58].(iii) Protected the murine hippocampal HT22 cells against glutamate-induced neurotoxicity, attenuated ROS generations through preserving the activities of SOD, GR, and GSH-Px [32].(iv) Inhibited clonogenic growth of human breast cancer cells cocultured with fibroblasts.(v) Inhibited prostate tumor growth in vitro and in vivo [59]. (vi) Inhibited NF-κB activation and reduces infiltration of inflammatory cells and apoptosis after spinal cord injury in rats [60].
Fisetin
(i) Exhibited antibacterial effect [61].(ii) Protected cultured rat liver epithelial-like cells against AFB-1-induced cytotoxicity and inhibited the binding of [3] AFB-1 to cellular DNA [62].(iii) Exhibited a predilection to inhibit histamine release stimulated by IgE-dependent ligands (antigen, anti-IgE, and con A) [63].(iv) Inhibited TPA-caused epidermal ornithine decarboxylase induction and tumor promotion in relation to lipoxygenase inhibition [64].(v) Inhibited PKC, almost 100% inhibition at a concentration of 100 micro-M from rat brain [65].(vi) Suppressed mutagenesis in Salmonella typhimurium strain TA100 NR induced by direct-acting carcinogen N-methyl-N′-nitro-N-nitrosoguanidine [66]. (vii) Showed topoisomerase II dependent DNA cleavage activity [67].(viii) Inhibited platelet aggregation [68]. Attenuated NO production in C6 astrocyte cell [69].(ix) Blocked glucose uptake in myelocytic U937 cells [70].(x) Inhibited corneal neovascularization; corneal blood vessels were induced by intrastromal implantation of pellets containing bFGF [71].(xi) Inhibited the proliferation of HSC-T6 cells, hepatic stellate cells stimulated by serum, MCM, and PDGF [72].(xii) Inducted apoptosis through activation of caspase-3 cascade and alternative expression of p21 protein in hepatocellular carcinoma cells SK-HEP-1 [73].(xiii) Exhibited antiviral activities against IHNV and VHSV [31].(xiv) Inhibited IL-4 and IL-13 synthesis and production by allergen- or anti-IgE-antibody-stimulated basophils [74].(xv) Protected against hepatosteatosis in mice by inhibiting miR-378 [75].(xvi) Protected bone by repressing NF-κB and MKP-1-dependent signaling pathways in osteoclasts [76].(xvii) Enhanced behavioral performances and attenuated reactive gliosis and inflammation during aluminum chloride-induced neurotoxicity [77].(xviii) Recuperated antioxidant status and protected hepatocellular ultrastructure from hyperglycemia mediated oxidative stress in STZ-induced rats diabetes model [78].
Kaempferol
(i) Inhibited estrogen binding to serum alpha-fetoprotein AFP in fetal or neonatal rats [79].(ii) Showed antioxidative activity against metal-induced lipid peroxidation [80]. (iii) Suppressed TNF-α-stimulated E-selectin expression on HUVECs [81]. (iv) Exhibited high inhibitory potencies for the 20alpha-HSD activity on liver cytosol of male mice [82].(v) Inhibited IgE or PMACI-mediated histamine release in RBL-2H3 cells and inhibited elevation of intracellular calcium [83].
Fustin
(i) Exerted inhibition of cell proliferation on Molt-4 cell and normal lymphocyte and enhanced IL-2 level [84].(ii) Suppressed 6-OHDA-induced cell death, blocked 6-OHDA-induced increases in ROS, [Ca(2+)](i), Bax/Bcl-2 ratio, caspase-3 activity, and p38 phosphorylation [34]. (iii) Attenuated Abeta(1–42)-impaired learning [85].(iv) Displayed antiviral activities against IHNV and VHSV [31].
Sulfuretin
(i) Exhibited potent antioxidants in a DPPH free radical scavenging assay [86].(ii) Exhibited aldose reductase and advanced glycation end-products inhibition [57].(iii) Inhibited iNOS and COX-2 protein and mRNA expression and reduced iNOS-derived NO, COX-derived PGE2, TNF-α, and IL-1β production in LPS-stimulated RAW264.7 and murine peritoneal macrophages [21].(iv) Reduced cytokine (IL-1 β- and IFN-γ-) induced NF-κB activation, iNOS expression, and NO production in rat insulinoma RINm5F cells, and prevented STZ-induced hyperglycemia and hypoinsulinemia by suppression of NF-κB activation [87].(v) Inhibited NF-κB pathway, suppressed the production of various cytokines in bronchoalveolar fluid and mucin production, and prevented the development of airway hyperresponsiveness on an ovalbumin-induced airway inflammation model in mice [88].(vi) Induced apoptosis through activation of Fas, caspase-8, and the mitochondrial death pathway in HL-60 human leukemia cells [89].(vii) Blocked NF-κB pathway in rheumatoid joints and reduced inflammatory responses and joint destruction [90].(viii) Inhibited TPA-induced NF-κB activation, MMP-9 expression, and cell invasion in MCF-7 cells [91].(ix)Induced miR-30C, downregulated cyclins D1 and D2, and triggered cell death in human cancer cell [92].
Quercetin
(i) Induced apoptosis in colorectal tumor cellsvia EGF receptor signaling [93].(ii) Showed antioxidative activity against metal-induced lipid peroxidation [80].(iii) Induced glutathione S-transferase and increased the resistance of cells to hydrogen peroxide [94].(iv) Inhibited the proliferation of HSC-T6 cells and hepatic stellate cells stimulated by serum, MCM, and PDGF [72].(v) Inhibited the antigen-IgE-mediated TNF-α and IL-4 production from RBL-2H3 [95].(vi) Decreased the amount of myelin phagocytosed by a macrophage cell line [96].(vii) Induced apoptosis through the activation of caspase-3 and caspase-8 in human leukemia U937 cells [97].(viii) Inhibited Abeta fibril formation on neuronal HT22 murine neuroblastoma cells [98].(ix) Inhibited prokaryotic SssI DNMT- and human DNMT1-mediated DNA methylation [99].(x) Showed inhibitory effects against HSV-1 [100].(xi) Exhibited inhibitory potencies for the 20alpha-HSD activity using liver cytosol of male mice [82].(xii) Inhibited EGF-induced cell transformation of mouse epidermal JB6 Cl 41 cells [101].(xiii) Inhibited tumor invasion via suppressing PKC Δ/ERK/AP-1-dependent MMP-9 activation in breast carcinoma cells [102].(xiv) Inhibited the cell proliferation induced by 17-beta-estradiol in the E-screen assay (the evaluation of antiestrogenicity) [103].
20alpha-HSD: 20alpha-hydroxysteroid dehydrogenase; 6-OHDA: 6-hydroxydopamine; AFB1: aflatoxin B1; Abeta: amyloid-beta; AP-1: activator protein-1; COX-2: cyclooxygenase-2; DCR: disease control rate; DNA: deoxyribonucleic acid; DNMT: DNA methyltransferase; DPPH: 1,1-diphenyl-2-picrylhydrazyl; ECOG: European Cooperative Oncology Group; EGF: epidermal growth factor; bFGF: basic fibroblast growth factor; ERK: extracellular signal-regulated kinases; GHB: gamma-hydroxybutyrate; GR: glutathione reductase; GSH: glutathione; GSH-Px: glutathione peroxidase; HO: heme oxygenase; HSV-1: herpes simplex virus type 1; IkB-alpha: inhibitory kappa B-alpha; IHNV: infectious hematopoietic necrosis virus; IKK beta: I kappaB kinase beta; IL-1 beta: interleukin-1 beta; iNOS: inducible nitric oxide synthase; LPS: lipopolysaccharide; MCM: macrophage conditioned medium; MKP-1: mitogen-activated protein kinase phosphatase-1; MMP: matrix metalloproteinase; NF-kappaB: nuclear factor-kappaB; NO: nitric oxide; nrf2: nuclear factor E2-related factor 2; NSCLC: non-small-cell lung carcinoma; OS: overall survival; PDGF: platelet-derived growth factor; PFS: progression-free survival; PGE2: prostaglandin E2; PKC: protein kinase C; PMACI: phorbol-12-myristate 13-acetate and calcium ionophore A23187; ROS: reactive oxygen species; SOD: superoxide dismutase; STZ: streptozotocin; TNF-alpha: tumor necrosis factor-alpha; TPA: 12-O-tetradecanoylphorbol-13-acetate; VHSV: viral hemorrhagic septicemia virus.


### 4.3. Biological Activities of Urushiol fromRhus verniciflua Stokes

Urushiol is an allergen found in Anacardiaceae family, especiallyToxicodendron spp. In sensitive individuals, urushiol can cause an allergic response [112]. Many researchers concerned about this compound could induce allergy during disease treatment withRhus verniciflua Stokes. All clinical studies indicated in Section 3 used an urushiol-free extract ofRhus verniciflua Stokes and the treatment of this remedy has no severe adverse effect. However, this is still controversial because more detailed confirmative works on safety will be also required to use this remedy clinically in the world.


## 4.1. Chemical Composition ofRhus verniciflua Stokes

Rhus verniciflua Stokes is chemically diverse in composition. To date, around 40 compounds, primarily phenolic acids and flavonoids, have been identified from this remedy (Figure 2) [14, 18, 110]. Of these compounds, 3 are phenolic acid, 4 flavonols, 4 flavanonols, 3 flavones, 1 chalconoid, and 2 tannins. The most common constituents present inRhus verniciflua Stokes are butein, which is a chalconoid having antibacterial, antifungal, antitumor, and anti-inflammatory properties, quercetin, which is a flavonol having antiviral, antiasthma, anticancer, antiprostatitis, and anti-inflammatory properties, and sulfuretin, which is a flavanonol possessing antioxidative, antidiabetes, anticancer, antiviral, and anti-inflammatory properties. The chemical structure and high-pressure liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS) analysis of some other compounds identified fromRhus verniciflua Stokes are shown in Figures 2 and 3 [14, 18].Figure 2
Molecular structure of common constituents ofRhus verniciflua Stokes.High-performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS) analysis of constituents identified fromRhus verniciflua Stokes. (a) HPLC chromatogram of standard compounds (upper) and purifiedRhus verniciflua Stokes extract (lower) at 280 nm and 360 nm: 1: protocatechuic acid, 2:p-hydroxybenzoic acid, 3: caffeic acid, 4: chlorogenic acid, 5:p-coumaric acid, 6: phloretin-2-O-glucoside, 7: fustin, 8: kaempferol-3-O-glucoside, 9: sulfuretin, 10: quercetin, 11: butein, and 12: kaempferol. (b) LC-MS chromatogram of phenolic-rich EtOAC fraction fromRhus verniciflua Stokes extract: 1: gallic acid, 2: protocatechuic acid, 3–9: 8 unknown compounds, 11: fisetin, 12: sulfuretin, and 13: butein.
(a)
(b)


## 4.2. Biological Activities of Main Compounds fromRhus verniciflua Stokes

Many researches have been studied to identify the clinically active ingredient fromRhus verniciflua Stokes like aspirin which was first discovered from the bark of the willow tree in 1763 by Edward Stone of Wadham College, Oxford University. Recently flavonoids fromRhus verniciflua Stokes have been found to have various biological activities, including antiproliferative, anti-inflammatory, and apoptotic activities in human cancer cell lines andin vivo model. For example, butein fromRhus verniciflua Stokes inhibited clonogenic growth of human breast cancer cells cocultured with fibroblasts [16], human colon adenocarcinoma cell proliferation [111], and prostate tumor growthin vitro andin vivo [59]. Moreover, this chalcone exhibited inhibition of NF-κB activation and infiltration reduction of inflammatory cells and apoptosis after spinal cord injury in animal model [60]. In another study, this tetrahydroxychalcone protected the murine hippocampal HT22 cells against glutamate-induced neurotoxicity, attenuated reactive oxygen species (ROS) generations through preserving the activities of SOD, GR, and GSH-Px, showed inhibitory effects on LPS-induced NO production, and suppressed the expression of both iNOS and COX-2 in BV2 cells [32]. Other main compounds, kaempferol, quercetin, and fisetin, have similar biological activities, including antiproliferative, anti-inflammatory, antioxidative, and apoptotic activities in human cancer cell lines andin vivo model. Their activities could come from a similar structure base. Some of the other constituents in whichRhus verniciflua Stokes has shown biological activities are listed in Table 5.Table 5
Selected biological activities of selected main compounds fromRhus verniciflua Stokes.
Type
Effect
Butein
(i) Exhibited aldose reductase and advanced glycation end-products inhibition [57].(ii) Protected pancreatic beta cells (INS-1 cells) against cytokine-induced toxicity mediated by inhibition of NO formation at concentrations of 15–30 μM [58].(iii) Protected the murine hippocampal HT22 cells against glutamate-induced neurotoxicity, attenuated ROS generations through preserving the activities of SOD, GR, and GSH-Px [32].(iv) Inhibited clonogenic growth of human breast cancer cells cocultured with fibroblasts.(v) Inhibited prostate tumor growth in vitro and in vivo [59]. (vi) Inhibited NF-κB activation and reduces infiltration of inflammatory cells and apoptosis after spinal cord injury in rats [60].
Fisetin
(i) Exhibited antibacterial effect [61].(ii) Protected cultured rat liver epithelial-like cells against AFB-1-induced cytotoxicity and inhibited the binding of [3] AFB-1 to cellular DNA [62].(iii) Exhibited a predilection to inhibit histamine release stimulated by IgE-dependent ligands (antigen, anti-IgE, and con A) [63].(iv) Inhibited TPA-caused epidermal ornithine decarboxylase induction and tumor promotion in relation to lipoxygenase inhibition [64].(v) Inhibited PKC, almost 100% inhibition at a concentration of 100 micro-M from rat brain [65].(vi) Suppressed mutagenesis in Salmonella typhimurium strain TA100 NR induced by direct-acting carcinogen N-methyl-N′-nitro-N-nitrosoguanidine [66]. (vii) Showed topoisomerase II dependent DNA cleavage activity [67].(viii) Inhibited platelet aggregation [68]. Attenuated NO production in C6 astrocyte cell [69].(ix) Blocked glucose uptake in myelocytic U937 cells [70].(x) Inhibited corneal neovascularization; corneal blood vessels were induced by intrastromal implantation of pellets containing bFGF [71].(xi) Inhibited the proliferation of HSC-T6 cells, hepatic stellate cells stimulated by serum, MCM, and PDGF [72].(xii) Inducted apoptosis through activation of caspase-3 cascade and alternative expression of p21 protein in hepatocellular carcinoma cells SK-HEP-1 [73].(xiii) Exhibited antiviral activities against IHNV and VHSV [31].(xiv) Inhibited IL-4 and IL-13 synthesis and production by allergen- or anti-IgE-antibody-stimulated basophils [74].(xv) Protected against hepatosteatosis in mice by inhibiting miR-378 [75].(xvi) Protected bone by repressing NF-κB and MKP-1-dependent signaling pathways in osteoclasts [76].(xvii) Enhanced behavioral performances and attenuated reactive gliosis and inflammation during aluminum chloride-induced neurotoxicity [77].(xviii) Recuperated antioxidant status and protected hepatocellular ultrastructure from hyperglycemia mediated oxidative stress in STZ-induced rats diabetes model [78].
Kaempferol
(i) Inhibited estrogen binding to serum alpha-fetoprotein AFP in fetal or neonatal rats [79].(ii) Showed antioxidative activity against metal-induced lipid peroxidation [80]. (iii) Suppressed TNF-α-stimulated E-selectin expression on HUVECs [81]. (iv) Exhibited high inhibitory potencies for the 20alpha-HSD activity on liver cytosol of male mice [82].(v) Inhibited IgE or PMACI-mediated histamine release in RBL-2H3 cells and inhibited elevation of intracellular calcium [83].
Fustin
(i) Exerted inhibition of cell proliferation on Molt-4 cell and normal lymphocyte and enhanced IL-2 level [84].(ii) Suppressed 6-OHDA-induced cell death, blocked 6-OHDA-induced increases in ROS, [Ca(2+)](i), Bax/Bcl-2 ratio, caspase-3 activity, and p38 phosphorylation [34]. (iii) Attenuated Abeta(1–42)-impaired learning [85].(iv) Displayed antiviral activities against IHNV and VHSV [31].
Sulfuretin
(i) Exhibited potent antioxidants in a DPPH free radical scavenging assay [86].(ii) Exhibited aldose reductase and advanced glycation end-products inhibition [57].(iii) Inhibited iNOS and COX-2 protein and mRNA expression and reduced iNOS-derived NO, COX-derived PGE2, TNF-α, and IL-1β production in LPS-stimulated RAW264.7 and murine peritoneal macrophages [21].(iv) Reduced cytokine (IL-1 β- and IFN-γ-) induced NF-κB activation, iNOS expression, and NO production in rat insulinoma RINm5F cells, and prevented STZ-induced hyperglycemia and hypoinsulinemia by suppression of NF-κB activation [87].(v) Inhibited NF-κB pathway, suppressed the production of various cytokines in bronchoalveolar fluid and mucin production, and prevented the development of airway hyperresponsiveness on an ovalbumin-induced airway inflammation model in mice [88].(vi) Induced apoptosis through activation of Fas, caspase-8, and the mitochondrial death pathway in HL-60 human leukemia cells [89].(vii) Blocked NF-κB pathway in rheumatoid joints and reduced inflammatory responses and joint destruction [90].(viii) Inhibited TPA-induced NF-κB activation, MMP-9 expression, and cell invasion in MCF-7 cells [91].(ix)Induced miR-30C, downregulated cyclins D1 and D2, and triggered cell death in human cancer cell [92].
Quercetin
(i) Induced apoptosis in colorectal tumor cellsvia EGF receptor signaling [93].(ii) Showed antioxidative activity against metal-induced lipid peroxidation [80].(iii) Induced glutathione S-transferase and increased the resistance of cells to hydrogen peroxide [94].(iv) Inhibited the proliferation of HSC-T6 cells and hepatic stellate cells stimulated by serum, MCM, and PDGF [72].(v) Inhibited the antigen-IgE-mediated TNF-α and IL-4 production from RBL-2H3 [95].(vi) Decreased the amount of myelin phagocytosed by a macrophage cell line [96].(vii) Induced apoptosis through the activation of caspase-3 and caspase-8 in human leukemia U937 cells [97].(viii) Inhibited Abeta fibril formation on neuronal HT22 murine neuroblastoma cells [98].(ix) Inhibited prokaryotic SssI DNMT- and human DNMT1-mediated DNA methylation [99].(x) Showed inhibitory effects against HSV-1 [100].(xi) Exhibited inhibitory potencies for the 20alpha-HSD activity using liver cytosol of male mice [82].(xii) Inhibited EGF-induced cell transformation of mouse epidermal JB6 Cl 41 cells [101].(xiii) Inhibited tumor invasion via suppressing PKC Δ/ERK/AP-1-dependent MMP-9 activation in breast carcinoma cells [102].(xiv) Inhibited the cell proliferation induced by 17-beta-estradiol in the E-screen assay (the evaluation of antiestrogenicity) [103].
20alpha-HSD: 20alpha-hydroxysteroid dehydrogenase; 6-OHDA: 6-hydroxydopamine; AFB1: aflatoxin B1; Abeta: amyloid-beta; AP-1: activator protein-1; COX-2: cyclooxygenase-2; DCR: disease control rate; DNA: deoxyribonucleic acid; DNMT: DNA methyltransferase; DPPH: 1,1-diphenyl-2-picrylhydrazyl; ECOG: European Cooperative Oncology Group; EGF: epidermal growth factor; bFGF: basic fibroblast growth factor; ERK: extracellular signal-regulated kinases; GHB: gamma-hydroxybutyrate; GR: glutathione reductase; GSH: glutathione; GSH-Px: glutathione peroxidase; HO: heme oxygenase; HSV-1: herpes simplex virus type 1; IkB-alpha: inhibitory kappa B-alpha; IHNV: infectious hematopoietic necrosis virus; IKK beta: I kappaB kinase beta; IL-1 beta: interleukin-1 beta; iNOS: inducible nitric oxide synthase; LPS: lipopolysaccharide; MCM: macrophage conditioned medium; MKP-1: mitogen-activated protein kinase phosphatase-1; MMP: matrix metalloproteinase; NF-kappaB: nuclear factor-kappaB; NO: nitric oxide; nrf2: nuclear factor E2-related factor 2; NSCLC: non-small-cell lung carcinoma; OS: overall survival; PDGF: platelet-derived growth factor; PFS: progression-free survival; PGE2: prostaglandin E2; PKC: protein kinase C; PMACI: phorbol-12-myristate 13-acetate and calcium ionophore A23187; ROS: reactive oxygen species; SOD: superoxide dismutase; STZ: streptozotocin; TNF-alpha: tumor necrosis factor-alpha; TPA: 12-O-tetradecanoylphorbol-13-acetate; VHSV: viral hemorrhagic septicemia virus.


## 4.3. Biological Activities of Urushiol fromRhus verniciflua Stokes

Urushiol is an allergen found in Anacardiaceae family, especiallyToxicodendron spp. In sensitive individuals, urushiol can cause an allergic response [112]. Many researchers concerned about this compound could induce allergy during disease treatment withRhus verniciflua Stokes. All clinical studies indicated in Section 3 used an urushiol-free extract ofRhus verniciflua Stokes and the treatment of this remedy has no severe adverse effect. However, this is still controversial because more detailed confirmative works on safety will be also required to use this remedy clinically in the world.


## 5. Conclusion

Whereas modern medicine has developed chemotherapy drugs for single-targeted agents, the traditional medicine is for multitargeted agents. The usage of entire or part extraction from a plant probably alleviates the adverse effects and drug resistance which are major problems in modern medicine [113].Rhus verniciflua Stokes is an Asian tree species of genusToxicodendron, which belongs to Anacardiaceae family. Traditionally, the remedy has been used for enormous purposes since ancient times. Modern science has provided the molecular basis for the properties ofRhus verniciflua Stokes against human diseases usingin vitro andin vivo model, and the existing human studies have provided a logical basis for further investigation of this remedy for the prevention and treatment of human diseases, especially cancer. In addition to this accomplishment, clinical studies have demonstrated the safety and efficacy ofRhus verniciflua Stokes in human subjects. The absence of any significant adverse effect associated with this remedy has made it superior to others. However, future studies should focus on employing larger, high-quality clinical trials, demonstrating its efficacy in terms of cancer patients’ survival and quality of life, and measuring cost-effectiveness in clinical practice. Additionally,Rhus verniciflua Stokes is a rich source of numerous biologically active constituents such as flavonoids, phenolic compounds which have anti-inflammatory activity, and chalconoids, which have antibacterial, antifungal, antitumor, and anti-inflammatory properties.



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*Source: 154561-2014-06-12.xml*