# Development and Validation of Selective High-Performance Liquid Chromatographic Method Using Photodiode Array Detection for Estimation of Aconitine in Polyherbal Ayurvedic Taila Preparations

Authors: Nitin Dubey; Nidhi Dubey; Rajendra Mehta
Journal: Chromatography Research International
 (2012)

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## Abstract

A simple, sensitive, and selective high-performance liquid chromatographic (HPLC) method has been developed and validated for the analysis of aconitine in marketed ayurvedictaila (oil) formulations containing roots of Aconitum chasmanthum. Chromatography of methanolic extracts of these formulations was performed on C18 (5 μm × 25 cm × 4.6 mm i.d.) column using isocratic mobile phase consisting of (65 : 35% v/v) acetonitrile and buffer solution (aqueous 0.01 M ammonium bicarbonate buffer, adjusted to pH 9.6 using 30% ammonia solution) at a flow rate of 1 mL/min and SPD-10 AVP photodiode array (PDA) UV-Visible detector. The analytical reference, aconitine, was quantified at 238 nm. The retention time of aconitine was about 42.54 min. The linear regression analysis data for the calibration plot showed a good linear relationship with correlation coefficient of 0.9989 in the concentration range of 15 to 90 μg/mL for aconitine with respect to peak area. The limit of detection and limit of quantitation values were found to be 0.03 μg/mL and 0.1 μg/mL respectively. Repeatability of the method was found to be 0.551–1.689 RSD. Recovery values from 97.75 to 99.91% indicate excellent accuracy of the method. The developed HPLC method is accurate and precise and it can be successfully applied for the determination of aconitine in marketed ayurvedic oil formulations containing Aconitum chasmanthum.


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## Body

## 1. Introduction

Aconitum chasmanthum(Family, Ranunculaceae) is a most valuable medicinal plant, widely used in the traditional and folk medicines of a number of countries of south east Asia. The chief chemical constituents of Aconitum chasmanthum root are aconitine, mesaconitine, and hypaconitine, and their respective hydrolyzed analogs are called monoester alkaloids, that is, benzoylaconine, benzoylmesaconine, and benzoylhypaconine [1–3]. Aconitine being the major diterpenoid responsible for the biopotency of Aconitum chasmanthum is recognized as the reference compound [3, 4]. Many polyherbal oil formulations in Indian and Chinese traditional systems of medicine used for control of skin diseases contain aconitum root as major active ingredient [5, 6]. Polyherbal oil formulations are made with the main objective of incorporating the fat-soluble fraction of the component of herbal drugs to a suitable oil base. Standardization of these formulations in terms of composition is important to ensure quality and safety. Aconitine can be used as analytical reference in the quality control of polyherbal oil formulations containing Aconitum chasmanthum[7–10].There are reports on the application of various analytical methods for isolation and quantitation of aconitine present inAconitum chasmanthum, biological fluids and other botanical sources [10–14]. Its estimation in polyherbal traditional medicines especially oil formulation is challenging. But no reported method deals with estimation of aconitine in complex matrix of ayurvedic taila formulations. High-performance liquid chromatography has emerged as an efficient tool for the phytochemical evaluation of herbal drugs because of its simplicity, sensitivity, accuracy, suitability for high-throughput screening, and so forth [15–21]. Hence it was thought worthwhile to develop a simple chromatographic method for determination of aconitine in ayurvedic taila formulations. The method was validated and found to be sensitive and reproducible [22, 23].


## 2. Material and Methods

### 2.1. Reference Compound and Reagents

Reference aconitine (95% w/v purity) was purchased from Sigma-Aldrich (Germany). All the chemicals used in analysis were of AR grade except those used for HPLC analyses which were of HPLC grade. All A.R. grade chemicals were procured from S.D. fine chemicals, Mumbai and all HPLC grade solvents were procured from Merck, Mumbai.


### 2.2. Polyherbal Oil Formulations

Commercial marketed ayurvedic polyherbal oil formulations “Varnaraksasa taila” and “Vipritmalla taila” which contain Aconitum chasmanthumas one of the components were selected for studies. Vranaraksasa  taila is a polyherbomineral oil containing mercury, sulphur, cinnabar (HgS), realgar (As4S4), fine copper powder,orpiment (As2S3), Allium sativum bulb (family, Alliaceae), and Aconitum chasmanthumroot (family, Ranunculaceae) digested in mustard seed oil (Brassica nigra. Family, Brassicaceae) as per classical Ayurvedic text [5, 6]. Vipritmalla taila contains cinnabar (HgO), Saussurea lapparoot (family, Asteraceae), Aconitum chasmanthum root (family, Ranunculaceae), Ferula  asafetidaresin (family, Apiaceae), Allium sativumbulb (family, Alliaceae), Plumbago zylenicumroot (family, Plumbaginaceae), Valeriana wallichiiroot (family, Valerianaceae), and Gloriosa superbaroot (family, Liliaceae) digested in mustard seed oil as per classical Ayurvedic text [5, 6].Samples of the same formulations in triplicate, manufactured by three different reputed ayurvedic drug manufacturers were collected from retail pharmacies in Indore, Madhya Pradesh, India.


### 2.3. Preparation of Standard Solutions

The stock solution of 1 mg/mL in methanol was prepared after keeping the purity of reference aconitine into consideration. Solution was filtered through Whatman filter paper (no. 1). Aliquots of stock solution were diluted to 5 mL using methanol to obtain working standards in concentration range from 15 to 90μg/mL.


### 2.4. Chromatographic Conditions

The mobile phase consisted of acetonitrile: aqueous 0.01 M ammonium bicarbonate buffer, adjusted to pH 9.6 using ammonia solution (65 : 35% v/v) at a flow rate of 1 mL/min. Before use, the mobile phase was degassed by an ultrasonic bath and filtered using 0.4μm membrane filter. Separation was performed at room temperature on HPLC system having a pump (Shimadzu LC 10ATVP) with 20 μL Rheodyne injector, Phenomenex Luna C18 (5 μm × 25 cm × 4.6 mm i.d) column, and SPD-10 AVP photodiode array (PDA) UV-Visible detector set at 238 nm and equipped with CLASS-VP software (Shimadzu. Kyoto, Japan).


### 2.5. HPLC Analysis of Ayurvedic Taila Preparations

Oil formulations (10 gm) were homogenated using homogenizer (Scientific instruments ltd, Indore) using methanol (50 mL) in proportion of 1 : 5, w/v at 50°C for 20 min. The mixture was centrifuged at 2000 rpm for 20 min at 4°C and the supernatant was collected. The residue was resuspended in methanol and the extraction was repeated five more times similarly. The supernatants were pooled and concentrated under vacuum at room temperature and made up to a volume of 20 mL using methanol. The extracts were filtered through 0.45μm filter and HPLC was performed under the conditions optimized for the reference compound. The amount of aconitine was quantified using calibration curves plotted with the reference compound.


### 2.6. Validation of Method

(a) Calibration Graph (Linearity of the HPLC Method)
The calibration curve was obtained at 6 concentration levels of aconitine standard solutions (15–90μg/mL). The solutions (20 μL) were injected into the HPLC system (n=6) with the chromatographic conditions previously given. The linearity was evaluated by the least-squares regression method.(b) Limits of Detection and Quantification
For determination of the limit of detection (LOD) and the limit of quantification (LOQ) different dilutions of the standard solution of aconitine were analyzed using mobile phase as the blank. The LOD and LOQ were determined on the basis of signal-to-noise ratio until the average responses were approximately 3 and 10 times the responses of the blank, respectively.(c) Accuracy (Recovery)
The accuracy of the methods was determined by calculating recovery of aconitine by the standard addition method. Known amounts of standard solution of aconitine (at three levels 50%, 100%, 150%) were added to prequantitated sample solutions. The amount of aconitine was estimated by applying values of peak area to the regression equations of the calibration graph. Five replicate samples of each concentration level were prepared.(d) Method Precision (Repeatability)
The precision of the instruments was checked by repeatedly injecting and analyzing (n=6) standard solutions of aconitine (45 μg/mL). The results are reported in terms of relative standard deviation (RSD).(e) Intermediate Precision (Reproducibility)
The intraday and interday precision of the proposed method were determined by analyzing standard solution of aconitine at 3 different concentrations (15, 45, and 90μg/mL) three times on the same day and on three different days. The results are reported in terms of RSD.(f) Solution Stability and Mobile Phase Stability
Solution stability in the assay method was evaluated by leaving test solutions of sample and reference standard in tightly capped volumetric flasks at room temperature in the dark for 24 h. The same sample solutions were assayed every 6 h interval in the study period. Mobile phase stability was studied by assaying the freshly prepared sample solutions against freshly prepared reference standard solutions at 6 h intervals up to 24 h. Mobile phase was prepared and kept constant during the study period. The relative standard deviation (RSD) of the assay of aconitine was calculated for the study period during mobile phase and solution stability experiments.


### 2.7. Statistical Analysis

The statistical analysis was performed using Microsoft Excel 2003.


## 2.1. Reference Compound and Reagents

Reference aconitine (95% w/v purity) was purchased from Sigma-Aldrich (Germany). All the chemicals used in analysis were of AR grade except those used for HPLC analyses which were of HPLC grade. All A.R. grade chemicals were procured from S.D. fine chemicals, Mumbai and all HPLC grade solvents were procured from Merck, Mumbai.


## 2.2. Polyherbal Oil Formulations

Commercial marketed ayurvedic polyherbal oil formulations “Varnaraksasa taila” and “Vipritmalla taila” which contain Aconitum chasmanthumas one of the components were selected for studies. Vranaraksasa  taila is a polyherbomineral oil containing mercury, sulphur, cinnabar (HgS), realgar (As4S4), fine copper powder,orpiment (As2S3), Allium sativum bulb (family, Alliaceae), and Aconitum chasmanthumroot (family, Ranunculaceae) digested in mustard seed oil (Brassica nigra. Family, Brassicaceae) as per classical Ayurvedic text [5, 6]. Vipritmalla taila contains cinnabar (HgO), Saussurea lapparoot (family, Asteraceae), Aconitum chasmanthum root (family, Ranunculaceae), Ferula  asafetidaresin (family, Apiaceae), Allium sativumbulb (family, Alliaceae), Plumbago zylenicumroot (family, Plumbaginaceae), Valeriana wallichiiroot (family, Valerianaceae), and Gloriosa superbaroot (family, Liliaceae) digested in mustard seed oil as per classical Ayurvedic text [5, 6].Samples of the same formulations in triplicate, manufactured by three different reputed ayurvedic drug manufacturers were collected from retail pharmacies in Indore, Madhya Pradesh, India.


## 2.3. Preparation of Standard Solutions

The stock solution of 1 mg/mL in methanol was prepared after keeping the purity of reference aconitine into consideration. Solution was filtered through Whatman filter paper (no. 1). Aliquots of stock solution were diluted to 5 mL using methanol to obtain working standards in concentration range from 15 to 90μg/mL.


## 2.4. Chromatographic Conditions

The mobile phase consisted of acetonitrile: aqueous 0.01 M ammonium bicarbonate buffer, adjusted to pH 9.6 using ammonia solution (65 : 35% v/v) at a flow rate of 1 mL/min. Before use, the mobile phase was degassed by an ultrasonic bath and filtered using 0.4μm membrane filter. Separation was performed at room temperature on HPLC system having a pump (Shimadzu LC 10ATVP) with 20 μL Rheodyne injector, Phenomenex Luna C18 (5 μm × 25 cm × 4.6 mm i.d) column, and SPD-10 AVP photodiode array (PDA) UV-Visible detector set at 238 nm and equipped with CLASS-VP software (Shimadzu. Kyoto, Japan).


## 2.5. HPLC Analysis of Ayurvedic Taila Preparations

Oil formulations (10 gm) were homogenated using homogenizer (Scientific instruments ltd, Indore) using methanol (50 mL) in proportion of 1 : 5, w/v at 50°C for 20 min. The mixture was centrifuged at 2000 rpm for 20 min at 4°C and the supernatant was collected. The residue was resuspended in methanol and the extraction was repeated five more times similarly. The supernatants were pooled and concentrated under vacuum at room temperature and made up to a volume of 20 mL using methanol. The extracts were filtered through 0.45μm filter and HPLC was performed under the conditions optimized for the reference compound. The amount of aconitine was quantified using calibration curves plotted with the reference compound.


## 2.6. Validation of Method

(a) Calibration Graph (Linearity of the HPLC Method)
The calibration curve was obtained at 6 concentration levels of aconitine standard solutions (15–90μg/mL). The solutions (20 μL) were injected into the HPLC system (n=6) with the chromatographic conditions previously given. The linearity was evaluated by the least-squares regression method.(b) Limits of Detection and Quantification
For determination of the limit of detection (LOD) and the limit of quantification (LOQ) different dilutions of the standard solution of aconitine were analyzed using mobile phase as the blank. The LOD and LOQ were determined on the basis of signal-to-noise ratio until the average responses were approximately 3 and 10 times the responses of the blank, respectively.(c) Accuracy (Recovery)
The accuracy of the methods was determined by calculating recovery of aconitine by the standard addition method. Known amounts of standard solution of aconitine (at three levels 50%, 100%, 150%) were added to prequantitated sample solutions. The amount of aconitine was estimated by applying values of peak area to the regression equations of the calibration graph. Five replicate samples of each concentration level were prepared.(d) Method Precision (Repeatability)
The precision of the instruments was checked by repeatedly injecting and analyzing (n=6) standard solutions of aconitine (45 μg/mL). The results are reported in terms of relative standard deviation (RSD).(e) Intermediate Precision (Reproducibility)
The intraday and interday precision of the proposed method were determined by analyzing standard solution of aconitine at 3 different concentrations (15, 45, and 90μg/mL) three times on the same day and on three different days. The results are reported in terms of RSD.(f) Solution Stability and Mobile Phase Stability
Solution stability in the assay method was evaluated by leaving test solutions of sample and reference standard in tightly capped volumetric flasks at room temperature in the dark for 24 h. The same sample solutions were assayed every 6 h interval in the study period. Mobile phase stability was studied by assaying the freshly prepared sample solutions against freshly prepared reference standard solutions at 6 h intervals up to 24 h. Mobile phase was prepared and kept constant during the study period. The relative standard deviation (RSD) of the assay of aconitine was calculated for the study period during mobile phase and solution stability experiments.


## 2.7. Statistical Analysis

The statistical analysis was performed using Microsoft Excel 2003.


## 3. Result and Discussion

The literature revealed that methanol is preferred for extraction of aconitine fromAconitum chasmanthum[10–14]. The same was used for extraction of aconitine from oil formulations. It is advantageous as the base oil in all the selected ayurvedic oil formulations was immiscible in this solvent. The immiscibility of oil in solvent will help in reducing number of interfering components in further chromatographic development. Multiple extractions were carried out to ensure complete extraction.


### 3.1. Development of the HPLC Method

The method development and selection of a suitable mobile phase involved several trials because of the complexity of the chemical composition of the herbals and the affinities of the components towards various solvents. The proportions of the organic and aqueous phases were adjusted to obtain a simple assay method with a reasonable run time and suitable retention time. Further optimization of mobile phase was performed based on resolution, asymmetric factor, and theoretical plates obtained for aconitine. Different mobile phases were tried like methanol: acetonitrile: water (45 : 45 : 10) which gave broad peak for aconitine. Combination of acetonitrile: methanol (10 : 90) was tried which gave broad and tailed peak. A mixture of methanol: water (60 : 40) was tried, which gave unresolved peak at retention time 48.9 min. Under optimized conditions HPLC with C18 column and UV detector at 238 nm using mobile phase (acetonitrile: aqueous 0.01 M ammonium bicarbonate buffer, adjusted to pH 9.6 using 30% ammonia solution (65 : 35%v/v)) gave well-resolved symmetric band for aconitine from its oil formulation (Figure1). The resolution was found to be 1.7. Retention time was found to be around 42 minutes and aconitine appeared on chromatogram at 42.54 minutes. Retention time of aconitine was found to be of 42.54 minutes.Figure 1
HPLC chromatogram of a 20μL injection of 40 μg/mL reference aconitine at 238 nm.The method consumes less volume of HPLC solvents. When the same drug solution was injected 6 times, the retention time of the drug was found to be the same (Figure2).Figure 2
HPLC chromatogram of a 20μL injection of the sample of taila preparation at 238 nm.


### 3.2. Validation of Method

The calibration curve was prepared by plotting the peak area against aconitine concentration; it was found linear in the range of 15–90μg/mL. The regression equation was found as y=71.5x-24.5 (r2=0.9989), showing excellent linearity (Figure 3). The method was validated in terms of precision, repeatability, accuracy, and other validation parameters (Table 1). The repeatability of the HPLC method and the intermediate precisions for intraday and interday variations are given in Table 2. The LOD value was found to be 0.03 μg/mL, which is the concentration that yields a signal-to-noise (S/N) ratio of 3/1. The LOQ value under the described conditions was 0.1 μg/mL with an S/N ratio of 10 : 1. This confirmed the sensitivity for quantitation of aconitine in taila preparations. Recovery values from 97.7 to 99.9% indicate excellent accuracy of the method (Table 3). The RSD values of assay of aconitine during solution stability, mobile phase stability and robustness studies (Table 4) were within 2.0%. The data obtained in both experiments proves that the sample solutions and mobile phase used during assay were stable up to 24 h.Table 1
Summary of validation parameters and system suitability parameters.
ParametersObservations  ±  % RSD(n=06)Retention time (min)42.54±0.33Tailing factor1.1±0.1Resolution1.7±0.23Theoretical plates3213±0.42Linearity range (μg/mL)15–90Correlation coefficient(r2)0.9989Regression equationy=71.5x-24.5Limit of detection (μg/mL)0.03Limit of quantification (μg/mL)0.1Repeatability (% RSD,n=6)0.55–1.68Table 2
Precision studies data for aconitine.
Concentration (μg/mL)IntradayInterday*Peak area ± SD% RSD*Peak area ± SD% RSD*151101.6±16.71.521112.0±6.30.57453059.9±29.50.963063.0±29.50.96755282.5±42.30.805292.6±47.80.90*Relative standard deviation (% RSD,n=3).Table 3
Accuracy study of the proposed method for aconitine.
Amount detected in sample (μg/mL)Amount of standard drug added (μg/mL)Amount of drug recovered (μg/mL)*% Recovery*Varnaraksasa taila28.3027.6±0.497.7±1.628.31441.7±0.298.6±0.528.32856.2±0.399.9±0.628.34269.2±0.498.5±0.5Vipritmalla taila32.2031.8±0.298.7±0.632.21647.9±0.499.4±0.232.23265.4±0.3101.8±0.532.24880.4±0.5100.2±0.7*Mean value ± relative standard deviation (% RSD,n=3).Table 4
Results of robustness studies.
Parameter% RSD peak areaMobile phase composition1.22Amount of mobile phase0.83Temperature0.92Chamber saturation time0.71Chamber dimensions0.43Figure 3
Calibration curve of reference aconitine in blank oil sample matrix at 238 nm.


### 3.3. HPLC Analysis of Ayurvedic Taila Preparations

Quantitative estimation of aconitine in polyherbal oil formulations given in Table5 revealed variation in its content in different brands, which indicates the need of standardization of raw material used and uniformity in method of manufacturing to be followed by different ayurvedic manufacturers. The method developed here does not require separation of unsaponifiable matter for quantification as reported for some active ingredients in oil formulation. Oil extract can be directly used for analysis. Avoidance of long and tedious step therefore makes this method more amendable to the high-throughput screening.Table 5
Aconitine content found in varioustaila preparations.
SampleBrandAconitine content in oil (mg/100 gm)Varnaraksasa taila10.27±0.00320.28±0.00530.26±0.004Vipritmalla taila10.31±0.0520.32±0.0730.32±0.08*Mean ± relative standard deviation (RSD,n=3).


## 3.1. Development of the HPLC Method

The method development and selection of a suitable mobile phase involved several trials because of the complexity of the chemical composition of the herbals and the affinities of the components towards various solvents. The proportions of the organic and aqueous phases were adjusted to obtain a simple assay method with a reasonable run time and suitable retention time. Further optimization of mobile phase was performed based on resolution, asymmetric factor, and theoretical plates obtained for aconitine. Different mobile phases were tried like methanol: acetonitrile: water (45 : 45 : 10) which gave broad peak for aconitine. Combination of acetonitrile: methanol (10 : 90) was tried which gave broad and tailed peak. A mixture of methanol: water (60 : 40) was tried, which gave unresolved peak at retention time 48.9 min. Under optimized conditions HPLC with C18 column and UV detector at 238 nm using mobile phase (acetonitrile: aqueous 0.01 M ammonium bicarbonate buffer, adjusted to pH 9.6 using 30% ammonia solution (65 : 35%v/v)) gave well-resolved symmetric band for aconitine from its oil formulation (Figure1). The resolution was found to be 1.7. Retention time was found to be around 42 minutes and aconitine appeared on chromatogram at 42.54 minutes. Retention time of aconitine was found to be of 42.54 minutes.Figure 1
HPLC chromatogram of a 20μL injection of 40 μg/mL reference aconitine at 238 nm.The method consumes less volume of HPLC solvents. When the same drug solution was injected 6 times, the retention time of the drug was found to be the same (Figure2).Figure 2
HPLC chromatogram of a 20μL injection of the sample of taila preparation at 238 nm.


## 3.2. Validation of Method

The calibration curve was prepared by plotting the peak area against aconitine concentration; it was found linear in the range of 15–90μg/mL. The regression equation was found as y=71.5x-24.5 (r2=0.9989), showing excellent linearity (Figure 3). The method was validated in terms of precision, repeatability, accuracy, and other validation parameters (Table 1). The repeatability of the HPLC method and the intermediate precisions for intraday and interday variations are given in Table 2. The LOD value was found to be 0.03 μg/mL, which is the concentration that yields a signal-to-noise (S/N) ratio of 3/1. The LOQ value under the described conditions was 0.1 μg/mL with an S/N ratio of 10 : 1. This confirmed the sensitivity for quantitation of aconitine in taila preparations. Recovery values from 97.7 to 99.9% indicate excellent accuracy of the method (Table 3). The RSD values of assay of aconitine during solution stability, mobile phase stability and robustness studies (Table 4) were within 2.0%. The data obtained in both experiments proves that the sample solutions and mobile phase used during assay were stable up to 24 h.Table 1
Summary of validation parameters and system suitability parameters.
ParametersObservations  ±  % RSD(n=06)Retention time (min)42.54±0.33Tailing factor1.1±0.1Resolution1.7±0.23Theoretical plates3213±0.42Linearity range (μg/mL)15–90Correlation coefficient(r2)0.9989Regression equationy=71.5x-24.5Limit of detection (μg/mL)0.03Limit of quantification (μg/mL)0.1Repeatability (% RSD,n=6)0.55–1.68Table 2
Precision studies data for aconitine.
Concentration (μg/mL)IntradayInterday*Peak area ± SD% RSD*Peak area ± SD% RSD*151101.6±16.71.521112.0±6.30.57453059.9±29.50.963063.0±29.50.96755282.5±42.30.805292.6±47.80.90*Relative standard deviation (% RSD,n=3).Table 3
Accuracy study of the proposed method for aconitine.
Amount detected in sample (μg/mL)Amount of standard drug added (μg/mL)Amount of drug recovered (μg/mL)*% Recovery*Varnaraksasa taila28.3027.6±0.497.7±1.628.31441.7±0.298.6±0.528.32856.2±0.399.9±0.628.34269.2±0.498.5±0.5Vipritmalla taila32.2031.8±0.298.7±0.632.21647.9±0.499.4±0.232.23265.4±0.3101.8±0.532.24880.4±0.5100.2±0.7*Mean value ± relative standard deviation (% RSD,n=3).Table 4
Results of robustness studies.
Parameter% RSD peak areaMobile phase composition1.22Amount of mobile phase0.83Temperature0.92Chamber saturation time0.71Chamber dimensions0.43Figure 3
Calibration curve of reference aconitine in blank oil sample matrix at 238 nm.


## 3.3. HPLC Analysis of Ayurvedic Taila Preparations

Quantitative estimation of aconitine in polyherbal oil formulations given in Table5 revealed variation in its content in different brands, which indicates the need of standardization of raw material used and uniformity in method of manufacturing to be followed by different ayurvedic manufacturers. The method developed here does not require separation of unsaponifiable matter for quantification as reported for some active ingredients in oil formulation. Oil extract can be directly used for analysis. Avoidance of long and tedious step therefore makes this method more amendable to the high-throughput screening.Table 5
Aconitine content found in varioustaila preparations.
SampleBrandAconitine content in oil (mg/100 gm)Varnaraksasa taila10.27±0.00320.28±0.00530.26±0.004Vipritmalla taila10.31±0.0520.32±0.0730.32±0.08*Mean ± relative standard deviation (RSD,n=3).


## 4. Conclusion

A method for analysis ofAconitum chasmanthumusing aconitine as analytical reference in polyherbal oil formulation was developed. Proposed method does not require tedious steps of saponification for separation of fatty acid which are the major interfering component in analysis of oils. Further the method does not require any chemical transformation of active moiety aconitine and it is analyzed as such. The method was found to be simple, precise, specific, sensitive, and accurate. It can be used for routine quality control of polyherbal oil formulations containing Aconitum chasmanthum.



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*Source: 157916-2012-04-10.xml*