# Benzyl-1,2,4-triazoles as CB1 Cannabinoid Receptor Ligands: Preparation andIn Vitro Pharmacological Evaluation

Authors: Laura  Hernandez-Folgado; Juan  Decara; Fernando  Rodríguez de Fonseca; Pilar  Goya; Nadine  Jagerovic
Journal: International Journal of Medicinal Chemistry
 (2016)

---

## Abstract

In a previous study, we have identified 3-alkyl-1,5-diaryl-1H-1,2,4-triazoles to be a novel class of cannabinoid type 1 receptor (CB1R) antagonists. In order to expand the number of cannabinoid ligands with a central 1,2,4-triazole scaffold, we have synthesized a novel series of 1-benzyl-1H-1,2,4-triazoles, and some of them were evaluated by CB1R radioligand binding assays. Compound12a showed the most interesting pharmacological properties, possessing a CB1R affinity in the nanomolar range.


---

## Body

## 1. Introduction

Due to the potential therapeutic effects of cannabinoids that include antiemetic, analgesic, antiglaucoma, obesity treatment, alcoholism, bronchodilatation, and inflammation, a considerable number of cannabinoid ligands have been reported in recent years [1]. Their effects are mediated through G-protein coupled cannabinoid receptors, which are part of the endocannabinoid system (ECS) [2]. So far, two types of cannabinoid receptors, designated as CB1R and CB2R, have been well characterized, and three putative cannabinoid receptors, GPR55, GPR18, and GPR119, have been also proposed [3]. CB1R has been found in the peripheral and central nervous system, and CB2R is mainly present in the immune system. Cannabinoid ligands belong to families of diverse structural classes such as eicosanoids, classical and nonclassical ligands related to Δ9-tetrahydrocannabinol (THC), and heterocycles. Among the heterocycles family, pyrazoles [4] and aminoalkylindoles [5] are the most representative ligands.In our early research program, it was found that triazole motif was an attractive scaffold for cannabinoid activity [6]. We reported that the CB1R antagonist 5-(4-chlorophenyl)-1-(2,4-dichlorophenyl)-3-hexyl-1H-1,2,4-triazole (LH21) exhibited antiobesity activity inin vivo assays (Figure 1) [7–9]. Pyrazole [10] and pyrrole [11] cannabinoid ligands bearing a benzyl substituent on position N1 have been reported in the literature as CB2R antagonists (Figure 1). This prompted us to extend our previous investigation by synthesizing a series of 3-alkyl-5-aryl-1-benzyl-1H-1,2,4-triazoles in order to establish structure-activity relationships.Figure 1
Structure of the CB1R antagonist LH21 and the CB2R antagonists SR144528 andN-(1S,2R)-myrtanyl-5-(4-chloro-3-methylphenyl)-1-(-4-methylbenzyl)-1H-pyrrole-3-carboxamide.We describe herein the synthesis of new benzyl-1,2,4-triazoles [12] and present initial results from radioligand binding assays as part of our investigation on cannabinoid active compounds.


## 2. Materials and Methods

### 2.1. Chemistry

#### 2.1.1. General

All reagents and solvents were used as commercially received. EtOH was dried over magnesium. TLC was carried out by precoated silica-gel 60 F254 plates (Merck) and detection by UV light (254 nm). Flash-column chromatography was carried out by Kieselgel 60 (230–400 mesh; Merck). Medium pressure chromatography (MPLC) was carried out by Flash Master Personal system with prepacked silica-gel cartridges. The purity of the final compounds was determined by elemental analysis or analytical HPLC. Elemental analysis was performed on a Heraeus CHN-O rapid analyzer. Analyses indicated by the symbols of the elements or functions were within ±0.4% of the theoretical values, except compound6. Analytical HPLC was run on a Waters 6000 with Delta Pak C 18.5 mm, 300 Ǻ (3.9 × 150 mm) column, using an eluent Acetonitrile/H2O (0.05% H3PO4 + 0.04% TEA) in the proportion indicated in each case; flow rate used was 1 mL/min and the UV absorption was detected at a wavelength of 254 nm. HPLC analyses were within ≥90% of purity, except compound11b (81% purity). The mass spectra (electrospray positive mode) were determined on a MSD-Series 1100 Hewlett Packard instrument. Melting points (uncorrected) were determined with a Reichert Jung Thermovar apparatus. 1H and 13C NMR spectra were recorded on a Gemini 200, Varian 300 and 400 unity spectrometers using TMS as the internal standard. All chemical shifts are reported in ppm. For the assignment of the protons and carbons of the aromatic rings Scheme 1 is used.Scheme 1


#### 2.1.2. General Procedure for the Synthesis of1 and2

To a suspension of the corresponding nitrile (10 equiv) in dry EtOH (30–75 mL) NaOMe (1 equiv) was added. It was stirred at room temperature under N2 atmosphere for 48 h. Afterwards, ammonium chloride (10 equiv) was added, and the stirring was maintained for 24 more hours. Then, unreacted ammonium chloride was filtered off and the solvent was evaporated from the liquid layer. The white solid obtained was washed with Et2O, dried, and used in the next step without further purification.4-Chlorobenzimidamide Hydrochloride (
1
). Compound1 was prepared from 4-chlorobenzonitrile (10.00 g, 72.7 mmol), NaOMe (393 mg, 7.3 mmol), and ammonium chloride (3.90 g, 72.7 mmol). Yield: 4.34 g of1 (31%) as a white solid. Mp = 246-247°C (236–240°C (EtOH)). [13] 1H-NMR (CD3OD) δ: 8.02 (d, 2H, J=9.0 Hz, Ho); 7.84 (d, 2H, J=9.0 Hz, Hm). 13C-NMR (CD3OD) δ: 167.6 C=NH; 141.4 Cp; 130.8 and 130.7 Co and Cm; 128.2 Cipso. MS (ES+)m/z: 155 (100%) [M+H]+.4-Amidinopyridinium Hydrochloride (
2
). Compound2 was prepared from 4-cyanopyridine (2.50 g, 24.0 mmol), NaOMe (130 mg, 2.4 mmol), and ammonium chloride (1.28 g, 24.0 mmol). Yield: 3.30 g of2 (87%) as a white solid. Mp = 248-249°C. 1H-NMR (CD3OD) δ: 8.93 (d, 2H, J=6.2 Hz, Hm); 7.87 (d, 2H, J=6.2 Hz, Ho). 13C-NMR (CD3OD) δ: 166.9 C=NH; 151.7 Cm; 138.1 Cipso; 123.2 Co. MS (ES+)m/z: 122 (100%) [M+H]+.


#### 2.1.3. General Procedure for the Synthesis of3–5

To a solution of the corresponding amidinium salt (1.5 equiv) in dry EtOH (10–45 mL), NaOMe (1 equiv) in 10 mL of dry EtOH was added. The suspension was stirred at room temperature for 1 h. Then, the solid formed was filtered on Celite. Octanoic hydrazide (2 equiv) was added to the liquid layer and the mixture was stirred under reflux for 46–49 h. After cooling the reaction mixture, solvent was removedin vacuo. The residue was dissolved in CH2Cl2 and washed with water (3 × 20 mL). The organic layer was dried over anhydrous Na2SO4 and the solvent was removedin vacuo. The obtained residue was purified by MPLC using cyclohexane/EtOAc (3 : 1) as eluent, except for compound5 where cyclohexane/EtOAc (3 : 1 to 4 : 1) was used.3-Heptyl-5-phenyl-1H-1,2,4-triazole (
3
). Compound3 was prepared from benzamidine hydrochloride hydrate (857 mg, 5.5 mmol), octanoic hydrazide (581 mg, 3.6 mmol), and NaOMe (394 mg, 7.3 mmol). Yield: 683 mg of3 (78%) as a transparent oil. Mp = 129–132°C oxalate (to a solution of the free base in Et2O, a solution of oxalic acid in EtOAc was added; the white solid was filtered off, washed with EtOAc, and dried). 1H-NMR (CDCl3) δ: 10.65 (bs, 1H, NH); 7.96 (m, 2H, Ho); 7.34 (m, 3H, Hm and Hp); 2.69 (t, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.65 (p, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.16 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.79 (bt, 3H, J=6.3 Hz, CH3). 13C-NMR (CDCl3) δ: 160.4 and 160.0 C3 and C5; 129.8 Cipso; 129.5 Cp; 128.6 Cm; 126.4 Co; 31.5 CH2CH2CH2CH2CH2CH2CH3; 29.1 CH2CH2CH2CH2CH2CH2CH3; 28.8 CH2CH2CH2CH2CH2CH2CH3; 28.1 CH2CH2CH2CH2CH2CH2CH3; 27.0CH2CH2CH2CH2CH2CH2CH3; 22.5 CH2CH2CH2CH2CH2CH2CH3; 13.9 CH3. MS (ES+)m/z: 244 (100%) [M+H]+. Anal (C15H21N3·C2H2O4) % calculated (% found) C: 61.25 (61.41); H: 6.95 (7.12); N: 12.60 (12.62).5-(4-Chlorophenyl)-3-heptyl-1H-1,2,4-triazole (
4
). Compound4 was prepared from1 (1.00 g, 5.2 mmol), octanoic hydrazide (549 mg, 3.5 mmol), and NaOMe (375 mg, 7.0 mmol). Yield 392 mg of4 (40%) as a white solid. Mp = 108–111°C. 1H-NMR (CDCl3) δ: 7.91 (d, 2H, J=8.6 Hz, Ho); 7.34 (d, 2H, J=8.6 Hz, Hm); 2.72 (t, 2H, J=7.6 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.68 (p, 2H, J=7.6 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.20 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.82 (bt, 3H, J=6.5 Hz, CH3). 13C-NMR (CDCl3) δ: 160.4 and 159.5 C3 and C5; 135.5 Cipso; 128.9 Cm; 128.7 Cp; 127.7 Co; 31.6 CH2CH2CH2CH2CH2CH2CH3; 29.2 CH2CH2CH2CH2CH2CH2CH3; 28.8 CH2CH2CH2CH2CH2CH2CH3; 28.0 CH2CH2CH2CH2CH2CH2CH3; 26.9CH2CH2CH2CH2CH2CH2CH3; 22.5 CH2CH2CH2CH2CH2CH2CH3; 14.0 CH3. MS (ES+)m/z: 278 (100%) [M+H]+. Anal (C15H20ClN3) % calculated (% found) C: 64.85 (65.09); H: 7.26 (7.42); N: 15.13 (15.35).4-(3-Heptyl-1H-1,2,4-triazol-5-yl)pyridine (
5
) and N′-[imino(pyridin-4-yl)methyl]octa-nehydrazide (6). Compound5 was prepared from2 (2.00 g, 12.7 mmol), octanoic hydrazide (1.35 g, 8.5 mmol), and NaOMe (918 mg, 17.0 mmol). Yield: 459 mg of5 (23%) as a white solid and 1.61 g of6 (45%) as a white solid.5: Mp = 109–112°C. 1H-NMR (CDCl3) δ: 8.69 (d, 2H, J=6.1 Hz, Hm); 8.06 (d, 2H, J=6.1 Hz, Ho); 2.85 (t, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.78 (p, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.21 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.81 (bt, 3H, J=6.7 Hz, CH3). 13C-NMR (CDCl3) δ: 159.4 and 159.2 C3 and C5; 149.4 Cm; 139.5 Cipso; 121.0 Co; 31.5 CH2CH2CH2CH2CH2CH2CH3; 29.1 CH2CH2CH2CH2CH2CH2CH3; 28.8 CH2CH2CH2CH2CH2CH2CH3; 28.1 CH2CH2CH2CH2CH2CH2CH3; 26.7CH2CH2CH2CH2CH2CH2CH3; 22.5 CH2CH2CH2CH2CH2CH2CH3; 13.9 CH3. MS (ES+)m/z: 245 (100%) [M+H]+. Anal (C14H20N4) % calculated (% found) C: 68.82 (68.71); H: 8.25 (8.36); N: 22.93 (22.78).6: Mp = 135–138°C. 1H-NMR (CD3OD) δ: 8.67 (d, 2H, J=6.1 Hz, Hm); 7.94 (d, 2H, J=6.1 Hz, Ho); 2.41 (t, 2H, J=7.4 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.76 (m, 2H, CH2CH2CH2CH2CH2CH2CH3); 1.41 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0,99 (bt, 3H, J=6.0 Hz, CH3). 13C-NMR (CD3OD) δ: 172.7 CONH; 151.7 C=NH; 150.4 Cm; 144.1 Cipso; 122.9 Co; 35.7CH2CH2CH2CH2CH2CH2CH3; 32.9 CH2CH2CH2CH2CH2CH2CH3; 30.4 and 30.2 CH2CH2CH2CH2CH2CH2CH3; 27.1 CH2CH2CH2CH2CH2CH2CH3; 23.7 CH2CH2CH2CH2CH2CH2CH3; 14.4 CH3. MS (ES+)m/z: 263 (100%) [M+H]+. Anal (C14H22N4O·1/2HCl) % calculated (% found) C: 59.93 (59.10); H: 8.08 (8.11); N: 19.97 (20.45).Intermediate6 (1.00 g, 3.6 mmol) in dry EtOH (20 mL) reacted by refluxing with NaOMe (1.03 g, 19.0 mmol) for 4 days. Under this procedure,5 was obtained in 78% yield (676 mg).


#### 2.1.4. General Procedure for the Synthesis of7a–15a and7b–15b

To a solution of the 3,5-disubstituted triazole (1 equiv) in 40% NaOH aq solution (3–5 mL) and toluene (7–10 mL) (Bu)4NBr (0.05 equiv) were first added, and later the corresponding alkylating agent (1 equiv) was added. The reaction mixture was stirred at 80–90°C (bath temperature) for the reaction time indicated. Afterwards, organic layer was separated and the aqueous layer was extracted with CH2Cl2 (3 × 25 mL). The combined organic layers were dried over anhydrous Na2SO4 and the solvent was removedin vacuo. The residue was purified by MPLC [cyclohexane/EtOAc (9 : 1)], except for compound13a, which was purified by flash chromatography [CH2Cl2 → CH2Cl2/MeOH (60 : 1)].1-Benzyl-5-heptyl-3-phenyl-1H-1,2,4-triazole (
7a
) and 1-Benzyl-3-heptyl-5-phenyl-1H-1,2,4-triazole (
7b
). Compounds7a and7b were prepared from3 (100 mg, 0.4 mmol), benzyl bromide (52 μL, 0.4 mmol), and (Bu)4NBr (6 mg, 0.02 mmol); reaction time: 1.5 h. Yield: 106 mg of7a (78%) as a transparent oil, and 13 mg of7b (9%) as a transparent oil.7a: 1H-NMR (CDCl3) δ: 8.08 (m, 2H, Ph); 7.40 (m, 3H, Ph); 7.31 (m, 3H, Bn); 7.18 (m, 2H, Bn); 5.34 (s, 2H, CH2Ph); 2.69 (t, 2H, J=7.8 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.64 (p, 2H, J=7.5 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.22 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.85 (bt, 3H, J=6.1 Hz, CH3). 13C-NMR (CDCl3) δ: 160.8 C3; 157.0 C5; 135.7 Cipso Bn; 131.2 Cipso Ph; 128.9 Cm Bn and Cp Ph; 128.5 Cm Ph; 126.9 Co Bn; 126.3 Co Ph; 128.1 Cp Bn; 52.1 (CH2Ph); 31.6 CH2CH2CH2CH2CH2CH2CH3; 29.2 CH2CH2CH2CH2CH2CH2CH3; 28.8 CH2CH2CH2CH2CH2CH2CH3; 27.8 CH2CH2CH2CH2CH2CH2CH3; 26.2CH2CH2CH2CH2CH2CH2CH3; 22.5 CH2CH2CH2CH2CH2CH2CH3; 14.0 CH3. MS (ES+)m/z: 334 (100%) [M+H]+. Anal (C22H27N3) % calculated (% found) C: 79.24 (79.35); H: 8.16 (8.40); N: 12.60 (12.64).7b: 1H-NMR (CDCl3) δ: 7.53 (m, 2H, Ph); 7.41 (m, 3H, Ph); 7.30 (m, 3H, Bn); 7.14 (m, 2H, Bn); 5.35 (s, 2H, CH2Ph); 2.75 (t, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.78 (p, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.23 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.85 (bt, 3H, J=6.4 Hz, CH3). 13C-NMR (CDCl3) δ: 164.1 C3; 155.3 C5; 136.1 Cipso Bn; 130.2 Cipso Ph; 128.9 Cm Bn; 128.8 and 128.7 Co and Cm Ph; 126.7 Co Bn; 127.9 Cp Bn and Cp Ph; 52.4 CH2Ph; 31.8 CH2CH2CH2CH2CH2CH2CH3; 29.3 CH2CH2CH2CH2CH2CH2CH3; 29.0 CH2CH2CH2CH2CH2CH2CH3; 28.5 CH2CH2CH2CH2CH2CH2CH3; 28.3CH2CH2CH2CH2CH2CH2CH3; 22.6 CH2CH2CH2CH2CH2CH2CH3; 14.1 CH3. MS (ES+)m/z: 334 (100%) [M+H]+. Anal (C22H27N3) % calculated (% found) C: 79.24 (79.10); H: 8.16 (7.95); N: 12.60 (12.42).1-(4-Chlorobenzyl)-5-heptyl-3-phenyl-1H-1,2,4-triazole (
8a
) and 1-(4-Chlorobenzyl)-3-heptyl-5-phenyl-1H-1,2,4-triazole (
8b
). Compounds8a and8b were prepared from3 (73 mg, 0.3 mmol), 4-chlorobenzyl chloride (48 mg, 0.3 mmol), and (Bu)4NBr (6 mg, 0.02 mmol); reaction time: 11 h. Yield: 96 mg of8a (87%) as a yellow solid and 10 mg of8b (9%) as a transparent oil.8a: 1H-NMR (CDCl3) δ: 8.07 (m, 2H, Ho Ph); 7.37 (m, 3H, Hm and Hp Ph); 7.29 (d, 2H, J=8.4 Hz, Hm Bn); 7.11 (d, 2H, J=8.4 Hz, Ho Bn); 5.27 (s, 2H, CH2Ar); 2.67 (t, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.69 (p, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.24 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.85 (ta, 3H, J=6.9 Hz, CH3). 13C-NMR (CDCl3) δ: 161.0 C3; 156.9 C5; 134.2 Cipso Bn; 134.0 Cp Bn; 131.1 Cipso Ph; 129.0 and 128.3 Co Bn, Cm Bn and Cp Ph; 128.5 Cm Ph; 126.2 Co Ph; 51.3 CH2Ar; 31.5 CH2CH2CH2CH2CH2CH2CH3; 29.2 CH2CH2CH2CH2CH2CH2CH3; 28.8 CH2CH2CH2CH2CH2CH2CH3; 27.7 CH2CH2CH2CH2CH2CH2CH3; 26.1CH2CH2CH2CH2CH2CH2CH3; 22.5 CH2CH2CH2CH2CH2CH2CH3; 14.0 CH3. MS (ES+)m/z: 368 (100%) [M+H]+. Anal (C22H26ClN3) % calculated (% found) C: 71.82 (71.75); H: 7.12 (6.98); N: 11.42 (11.63).8b: 1H-NMR (CDCl3) δ: 7.50 (m, 2H, H Ph); 7.43 (m, 3H, Ph); 7.29 (d, 2H, J=8.4 Hz, Hm Bn); 7.06 (d, 2H, J=8.4 Hz, Ho Bn); 5.30 (s, 2H, CH2Ar); 2.75 (t, 2H, J=7.5 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.77 (p, 2H, J=7.5 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.23 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.85 (ta, 3H, J=6.2 Hz, CH3). 13C-NMR (CDCl3) δ: 164.6 C3; 155.5 C5; 134.7 Cipso Bn; 133.8 Cp Bn; 130.1 Cp Ph; 129.0 Cm Bn; 128.8 Co Bn; 128.6 Cm Ph; 128.1 Co Ph; 128.0 Cipso Ph; 51.7 CH2Ar; 31.8 CH2CH2CH2CH2CH2CH2CH3; 29.4 CH2CH2CH2CH2CH2CH2CH3; 29.0 CH2CH2CH2CH2CH2CH2CH3; 28.5CH2CH2CH2CH2CH2CH2CH3; 22.6 CH2CH2CH2CH2CH2CH2CH3; 14.1 CH3. MS (ES+)m/z: 368 (100%) [M+H]+. HPLC: Acetonitrile/H2O 95 : 5, tR=28.5 min (99% purity).1-(2,4-Dichlorobenzyl)-5-heptyl-3-phenyl-1H-1,2,4-triazole (
9a
) and 1-(2,4-Dichloro-benzyl)-3-heptyl-5-phenyl-1H-1,2,4-triazole (
9b
). Compounds9a and9b were prepared from3 (100 mg, 0.4 mmol), 2,4-dichlorobenzyl chloride (57 μL, 0.4 mmol), and (Bu)4NBr (6 mg, 0.02 mmol); reaction time: 5 h. Yield: 123 mg of9a (74%) as a white solid and 12 mg of9b (7%) as a transparent oil.9a: Mp = 70–73°C. 1H-NMR (CDCl3) δ: 8.11 (m, 2H, Ho Ph); 7.42 (m, 4H, Hm′ Bn, Hm and Hp Ph); 7.19 (dd, 1H,J = 8.3 Hz and 1.6 Hz, Hm Ph); 6.85 (d, 1H, J=8.3 Hz, Ho Bn); 5.41 (s, 2H, CH2Ar); 2.73 (t, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.73 (p, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.27 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.88 (bt, 3H, J=6.4 Hz, CH3). 13C-NMR (CDCl3) δ: 161.4 C3; 157.4 C5; 134.5 Cipso Bn; 132.8 Cp Bn; 132.2 Co′ Bn; 131.0 Cipso Ph; 129.3 Co Bn; 129.2 Cm′ Bn; 129.1 Cp Ph; 128.5 Cm Ph; 127.7 Cm Bn; 126.3 Co Ph; 48.6 CH2Ar; 31.5 CH2CH2CH2CH2CH2CH2CH3; 29.1 CH2CH2CH2CH2CH2CH2CH3; 28.8 CH2CH2CH2CH2CH2CH2CH3; 27.8 CH2CH2CH2CH2CH2CH2CH3; 26.0CH2CH2CH2CH2CH2CH2CH3; 22.5 CH2CH2CH2CH2CH2CH2CH3; 14.0 CH3. MS (ES+)m/z: 402 (100%) [M+H]+. Anal (C22H25Cl2N3) % calculated (% found) C: 65.67 (65.42); H: 6.26 (6.50); N: 10.44 (10.35).9b: 1H-NMR (CDCl3) δ: 7.50–7.43 (m, 5H, Ph); 7.40 (d, 1H, J=1.7 Hz, Hm′ Bn); 7.20 (dd, 1H, J=8.6 Hz and 1.7 Hz, Hm Bn); 6.84 (d, 1H, J=8.6 Hz, Ho Bn); 5.39 (s, 2H, CH2Ar); 2.76 (t, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.79 (p, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.23 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.85 (bt, 3H, J=6.1 Hz, CH3). 13C-NMR (CDCl3) δ: 165.2 C3; 156.2 C5; 134.6 Cipso Bn; 133.0 Cp Bn; 132.9 Co′ Bn; 130.5 Co Bn; 129.7 Cm′ Bn; 129.2 Cm Ph; 129.0 Cp Ph; 128.6 Co Ph; 127.9 Cm Bn; 127.8 Cipso Ph; 50.1 CH2Ar; 32.0 CH2CH2CH2CH2CH2CH2CH3; 29.6 CH2CH2CH2CH2CH2CH2CH3; 29.2 CH2CH2CH2CH2CH2CH2CH3; 28.7CH2CH2CH2CH2CH2CH2CH3; 22.9 CH2CH2CH2CH2CH2CH2CH3; 14.3 CH3. MS (ES+)m/z: 402 (100%) [M+H]+. Anal (C22H25Cl2N3·C6H12) % calculated (% found) C: 69.12 (69.42); H: 7.67 (7.79); N: 8.64 (8.24).1-Benzyl-3-(4-chlorophenyl)-5-heptyl-1H-1,2,4-triazole (
10a
) and 1-Benzyl-5-(4-chlo-rophenyl)-3-heptyl-1H-1,2,4-triazole (
10b
). Compounds10a and10b were prepared from4 (80 mg, 0.3 mmol), benzyl bromide (34 μL, 0.3 mmol), and (Bu)4NBr (6 mg, 0.02 mmol); reaction time: 20 min. Yield: 94 mg of10a (89%) as a white solid and 9 mg of10b (8%) as a yellow oil.10a: Mp = 47–50°C. 1H-NMR (CDCl3) δ: 8.02 (d, 2H, J=8.8 Hz, Ho Ar); 7.36 (d, 2H, J=8.8 Hz, Hm Ar); 7.29 (m, 3H, Bn); 7.19 (m, 2H, Bn); 5.31 (s, 2H, CH2Ph); 2.67 (t, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.66 (p, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.22 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.85 (bt, 3H, J=6.9 Hz, CH3). 13C-NMR (CDCl3) δ: 159.9 C3; 157.1 C5; 135.5 Cipso Ar; 134.7 Cipso Bn; 129.8 Cp Ar; 128.9 Cm Ar; 128.6 Cm Bn; 128.1 Cp Bn; 127.6 Co Ar; 126.9 Co Bn; 52.1 (CH2Ph); 31.5 CH2CH2CH2CH2CH2CH2CH3; 29.2 CH2CH2CH2CH2CH2CH2CH3, 28.8 CH2CH2CH2CH2CH2CH2CH3; 27.7 CH2CH2CH2CH2CH2CH2CH3; 26.1CH2CH2CH2CH2CH2CH2CH3; 22.5 CH2CH2CH2CH2CH2CH2CH3; 14.0 CH3. MS (ES+)m/z: 368 (100%) [M+H]+. Anal (C22H26ClN3) % calculated (% found) C: 71.82 (72.02); H: 7.12 (6.89); N: 11.42 (11.24).10b: 1H-NMR (CDCl3) δ: 7.47 (d, 2H, J=8.5 Hz, Hm Ar); 7.38 (d, 2H, J=8.5 Hz, Ho Ar); 7.30 (m, 3H, Bn); 7.00 (m, 2H, Bn); 5.33 (s, 2H, CH2Ph); 2.75 (t, 2H, J=7.6 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.78 (p, 2H, J=7.6 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.25 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.85 (bt, 3H, J=6.4 Hz, CH3). 13C-NMR (CDCl3) δ: 164.6 C3; 155.0 C5; 136.3 Cipso Ar; 136.2 Cipso Bn; 130.0 Cm Ar; 129.4 Cp Ar; 129.1 Co Ar; 129.0 Cm Bn; 128.0 Cp Bn; 52.6 (CH2Ph); 31.8 CH2CH2CH2CH2CH2CH2CH3; 29.4 CH2CH2CH2CH2CH2CH2CH3; 29.0 CH2CH2CH2CH2CH2CH2CH3; 28.5CH2CH2CH2CH2CH2CH2CH3; 22.6 CH2CH2CH2CH2CH2CH2CH3; 14.0 CH3. MS (ES+)m/z: 368 (100%) [M+H]+.1-(4-Chlorobenzyl)-3-(4-chlorophenyl)-5-heptyl-1H-1,2,4-triazole (
11a
) and 1-(4-Chlo-robenzyl)-5-(4-chlorophenyl)-3-heptyl-1H-1,2,4-triazole (
11b
). Compounds11a and11b were prepared from4 (100 mg, 0.4 mmol), 4-chlorobenzyl chloride (64 mg, 0.4 mmol), and (Bu)4NBr (6 mg, 0.02 mmol); reaction time: 6 h. Yield: 132 mg of11a (91%) as a white solid and 2 mg of11b (1%) as a transparent oil.11a: Mp = 58–60°C. 1H-NMR (CDCl3) δ: 8.00 (d, 2H, J=8.8 Hz, Ho Ar); 7.35 (d, 2H, J=8.8 Hz, Hm Ar); 7.28 (d, 2H, J=8.4 Hz, Hm CH2Ar); 7.10 (d, 2H, J=8.4 Hz, Ho CH2Ar); 5.26 (s, 2H, CH2Ar); 2.66 (t, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.66 (p, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.23 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.84 (bt, 3H, J=6.3 Hz, CH3). 13C-NMR (CDCl3) δ: 160.1 C3; 157.1 C5; 134.8 Cipso Ar; 134.1 Cipso CH2Ar; 134.0 Cp CH2Ar; 129.6 Cp Ar; 129.0 Cm Ar; 128.6 Cm CH2Ar; 128.3 Co CH2Ar; 127.5 Co Ar; 51.3 (CH2Ar); 31.5 CH2CH2CH2CH2CH2CH2CH3; 29.2 CH2CH2CH2CH2CH2CH2CH3; 28.8 CH2CH2CH2CH2CH2CH2CH3; 27.7 CH2CH2CH2CH2CH2CH2CH3; 26.1CH2CH2CH2CH2CH2CH2CH3; 22.5 CH2CH2CH2CH2CH2CH2CH3; 14.0 CH3. MS (ES+)m/z: 402 (100%) [M+H]+. Anal (C22H25Cl2N3) % calculated (% found) C: 65.67 (65.72); H: 6.26 (6.12); N: 10.44 (10.40).11b: 1H-NMR (CDCl3) δ: 7.45 (d, 2H, J=9.0 Hz, Ho Ar); 7.39 (d, 2H, J=9.0 Hz, Hm Ar); 7.30 (d, 2H, J=8.3 Hz, Hm CH2Ar); 7.05 (d, 2H, J=8.3 Hz, Ho CH2Ar); 5.29 (s, 2H, CH2Ar); 2.70 (t, 2H, J=7.8 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.75 (p, 2H, J=7.8 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.23 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.82 (bt, 3H, J=6.2 Hz, CH3). 13C-NMR (CDCl3) δ: 164.7 C3; 154.4 C5; 136.4 Cipso Ar; 134.4 Cipso CH2Ar; 134.0 Cp CH2Ar; 129.9 Cm and Cp Ar; 129.2 Co Ar and Cm CH2Ar; 128.0 Co CH2Ar; 51.8 CH2Ar; 31.8 CH2CH2CH2CH2CH2CH2CH3; 29.7 CH2CH2CH2CH2CH2CH2CH3; 29.3 CH2CH2CH2CH2CH2CH2CH3; 29.0 CH2CH2CH2CH2CH2CH2CH3; 28.4CH2CH2CH2CH2CH2CH2CH3; 22.6 CH2CH2CH2CH2CH2CH2CH3; 14.1 CH3. MS (ES+)m/z: 402 (100%) [M+H]+. HPLC: Acetonitrile/H2O 95 : 5, tR=25.5 min (81% purity).3-(4-Chlorophenyl)-1-(2,4-dichlorobenzyl)-5-heptyl-1H-1,2,4-triazole (
12a
) and 5-(4-Chlorophenyl)-1-(2,4-dichlorobenzyl)-3-heptyl-1H-1,2,4-triazole (
12b
). Compounds12a and12b were prepared from4 (90 mg, 0.3 mmol), 2,4-dichlorobenzyl chloride (45 μL, 0.3 mmol), and (Bu)4NBr (6 mg, 0.02 mmol); reaction time: 6 h. Yield: 122 mg of12a (86%) as a white solid and 8 mg of12b (6%) as a transparent oil.12a: Mp = 97–99°C. 1H-NMR (CDCl3) δ: 8.00 (d, 2H, J=8.6 Hz, Ho Ar); 7.41 (d, 1H, J=2.0 Hz, Hm′ CH2Ar); 7.37 (d, 2H, J=8.6 Hz, Hm Ar); 7.18 (dd, 1H, J=8.4 Hz and 2.0 Hz, Hm CH2Ar); 6.84 (d, 1H, J=8.4 Hz, Ho CH2Ar); 5.37 (s, 2H, CH2Ar); 2.70 (t, 2H, J=7.6 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.69 (p, 2H, J=7.6 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.22 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.85 (bt, 3H, J=7.1 Hz, CH3). 13C-NMR (CDCl3) δ: 160.5 C3; 157.6 C5; 135.0 Cipso Ar; 134.6 Cipso CH2Ar; 132.9 Cp CH2Ar; 132.0 Co′ CH2Ar; 129.5 Cp Ar; 129.4 Co CH2Ar; 129.3 Cm′ CH2Ar; 128.7 Cm Ar; 127.7 Cm CH2Ar; 127.6 Co Ar; 48.7 CH2Ar; 31.5 CH2CH2CH2CH2CH2CH2CH3; 29.1 CH2CH2CH2CH2CH2CH2CH3; 28.8 CH2CH2CH2CH2CH2CH2CH3; 27.7 CH2CH2CH2CH2CH2CH2CH3; 26.0CH2CH2CH2CH2CH2CH2CH3; 22.5 CH2CH2CH2CH2CH2CH2CH3; 14.0 CH3. MS (ES+)m/z: 436 (100%) [M+H]+. Anal (C22H24Cl3N3) % calculated (% found) C: 60.49 (60.42); H: 5.54 (5.74); N: 9.62 (9.42).12b: 1H-NMR (CDCl3) δ: 7.41 (bs, 5H, Ho Ar, Hm Ar and Hm′ CH2Ar); 7.21 (dd, 1H, J=8.3 Hz and 2.0 Hz, Hm CH2Ar); 6.84 (d, 1H, J=8.3 Hz, Ho CH2Ar); 5.37 (s, 2H, CH2Ar); 2.75 (t, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.77 (p, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1,23 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.85 (bt, 3H, J=6.1 Hz, CH3). 13C-NMR (CDCl3) δ: 165.1 C3; 154.9 C5; 136.6 Cipso Ar; 134.7 Cipso CH2Ar; 132.8 Cp CH2Ar; 132.5 Co′ CH2Ar; 129.7 Cm and Cp Ar; 129.6 Co CH2Ar; 129.3 Co Ar; 129.0 Cm′ CH2Ar; 127.8 Cm CH2Ar; 49.9 CH2Ar; 31.8 CH2CH2CH2CH2CH2CH2CH3; 29.7 CH2CH2CH2CH2CH2CH2CH3; 29.3 CH2CH2CH2CH2CH2CH2CH3; 29.0 CH2CH2CH2CH2CH2CH2CH3; 28.4CH2CH2CH2CH2CH2CH2CH3; 22.6 CH2CH2CH2CH2CH2CH2CH3; 14.0 CH3. MS (ES+)m/z: 436 (99%) [M+H]+. HPLC: Acetonitrile/H2O 90 : 10, tR=66.2 min (99% purity).4-(1-Benzyl-5-heptyl-1H-1,2,4-triazol-3-yl)pyridine (
13a
). Compound13a was prepared from5 (150 mg, 0.6 mmol), benzyl chloride (73 μL, 0.6 mmol), and (Bu)4NBr (6 mg, 0.02 mmol); reaction time: 2.5 h. Yield: 166 mg of13a (81%) as an orange oil. 1H-NMR (CDCl3) δ: 8.63 (d, 2H, J=6.0 Hz, Hm pyr); 7.93 (d, 2H, J=6.0 Hz, Ho pyr); 7.31 (m, 3H, Ph); 7.16 (m, 2H, Ph); 5.33 (s, 2H, CH2Ar); 2.68 (t, 2H, J=7.8 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.65 (p, 2H, J=7.0 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.21 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.83 (bt, 3H, J=7.1 Hz, CH3). 13C-NMR (CDCl3) δ: 158.7 C3; 157.5 C5; 150.1 Cm pyr; 138.5 Cipso pyr; 135.2 Cipso Ph; 128.9 Cm Ph; 128.2 Cp Ph; 127.0 Co Ph; 120.4 Co pyr; 52.3 CH2Ph; 31.5 CH2CH2CH2CH2CH2CH2CH3; 29.1 CH2CH2CH2CH2CH2CH2CH3; 28.8 CH2CH2CH2CH2CH2CH2CH3; 27.6 CH2CH2CH2CH2CH2CH2CH3; 26.1CH2CH2CH2CH2CH2CH2CH3; 22.5 CH2CH2CH2CH2CH2CH2CH3; 14.0 CH3. MS (ES+)m/z: 335 (100%) [M+H]+. Anal (C21H26N4) % calculated (% found) C: 75.41 (75.71); H: 7.84 (7.79); N: 16.75 (16.54).4-[1-(4-Chlorobenzyl)-5-heptyl-1H-1,2,4-triazol-3-yl]pyridine (
14a
) and 4-[1-(4-Chlo-robenzyl)-3-heptyl-1H-1,2,4-triazol-5-yl]pyridine (
14b
). Compounds14a and14b were prepared from5 (180 mg, 0.7 mmol), 4-chlorobenzyl chloride (119 mg, 0.7 mmol), and (Bu)4NBr (12 mg, 0.04 mmol); reaction time: 6 h. Yield: 150 mg of14a (51%) as a brown solid and 10 mg of14b (4%) as a brown oil.14a: Mp = 77–80°C. 1H-NMR (CDCl3) δ: 8.61 (d, 2H, J=6.0 Hz, Hm pyr); 7.90 (d, 2H, J=6.0 Hz, Ho pyr); 7.27 (d, 2H, J=8.6 Hz, Hm Ar); 7.09 (d, 2H, J=8.6 Hz, Ho Ar); 5.27 (s, 2H, CH2Ar); 2.66 (t, 2H, J=7.8 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.65 (p, 2H, J=7.8 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.20 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.82 (bt, 3H, J=6.6 Hz, CH3). 13C-NMR (CDCl3) δ: 158.9 C3; 157.5 C5; 150.2 Cm pyr; 138.4 Cipso pyr; 134.2 Cipso Ar; 133.7 Cp Ar; 129.1 Cm Ar; 128.4 Co Ar; 120.4 Co pyr; 51.8 CH2Ar; 31.5 CH2CH2CH2CH2CH2CH2CH3; 29.1 CH2CH2CH2CH2CH2CH2CH3; 28.8 CH2CH2CH2CH2CH2CH2CH3; 26.0CH2CH2CH2CH2CH2CH2CH3; 22.5 CH2CH2CH2CH2CH2CH2CH3; 14.0 CH3. MS (ES+)m/z: 369 (100%) [M+H]+. Anal (C21H25ClN4) % calculated (% found) C: 68.37 (68.55); H: 6.83 (6.90); N: 15.19 (15.21).14b: 1H-NMR (CDCl3) δ: 8.65 (d, 2H, J=6.2 Hz, Hm pyr); 7.44 (d, 2H, J=6.2 Hz, Ho pyr); 7.31 (d, 2H, J=8.5 Hz, Hm Ar); 7.06 (d, 2H, J=8.5 Hz, Ho pyr); 5.35 (s, 2H, CH2Ar); 2.76 (t, 2H, J=7.6 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.74 (p, 2H, J=7.6 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.27 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.85 (m, 3H, CH3). 13C-NMR (CDCl3) δ: 165.1 C3; 152.8 C5; 150.5 Cm pyr; 135.4 Cipso pyr; 134.2 Cipso Ar; 134.0 Cp Ar; 129.3 Cm Ar; 128.0 Co Ar; 122.5 Co pyr; 52.1 CH2Ar; 31.7 CH2CH2CH2CH2CH2CH2CH3; 29.3 CH2CH2CH2CH2CH2CH2CH3; 29.2 CH2CH2CH2CH2CH2CH2CH3; 28.4 and 29.0CH2CH2CH2CH2CH2CH2CH3; 22.6 CH2CH2CH2CH2CH2CH2CH3; 14.1 CH3. MS (ES+)m/z: 369 (100%) [M+H]+.4-[1-(2,4-Dichlorobenzyl)-5-heptyl-1H-1,2,4-triazol-3-yl]pyridine (
15a
) and 4-[1-(2,4-Dichlorobenzyl)-3-heptyl-1H-1,2,4-triazol-5-yl]pyridine (
15b
). Compounds15a and15b were prepared from5 (200 mg, 0.8 mmol), 2,4-dichlorobenzyl chloride (114 μL, 0.8 mmol), and (Bu)4NBr (12 mg, 0.04 mmol.); reaction time: 2 h. Yield: 118 mg of15a (36%) as a white solid and 7 mg of15b (2%) as a yellow oil.15a: Mp = 104-105°C. 1H-NMR (CDCl3) δ: 8.65 (d, 2H, J=6.1 Hz, Hm pyr); 7.93 (d, 2H, J=6.1 Hz, Ho pyr); 7.42 (d, 1H, J=2.2 Hz, Hm′ Ar); 7.19 (dd, 1H, J=8.4 Hz and 2.2 Hz, Hm Ar); 6.58 (d, 1H, J=8.4 Hz, Ho Ar); 5.39 (s, 2H, CH2Ar); 2.72 (t, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.70 (p, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.23 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.84 (bt, 3H, J=6.5 Hz, CH3). 13C-NMR (CDCl3) δ: 159.3 C3; 158.1 C5; 150.3 Cm pyr; 138.3 Cipso pyr; 134.8 Cipso Ar; 133.0 Cp Ar; 131.7 Co′ Ar; 129.5 Co and Cm′ Ar; 127.8 Cm Ar; 120.4 Co pyr; 48.9 (CH2Ar); 31.6 CH2CH2CH2CH2CH2CH2CH3; 29.1 CH2CH2CH2CH2CH2CH2CH3; 28.8 CH2CH2CH2CH2CH2CH2CH3; 27.7 CH2CH2CH2CH2CH2CH2CH3; 26.0CH2CH2CH2CH2CH2CH2CH3; 22.5 CH2CH2CH2CH2CH2CH2CH3; 14.0 CH3. MS (ES+)m/z: 403 (100%) [M+H]+. Anal (C21H24Cl2N4) % calculated (% found) C: 62.53 (62.28); H: 6.00 (6.30); N: 13.89 (14.04).15b: 1H-NMR (CDCl3) δ: 8.70 (d, 2H, J=6.1 Hz, Hm pyr); 7.43 (d, 1H, J=2.2 Hz, Hm′ Ar); 7.41 (d, 2H, J=6.1 Hz, Ho pyr); 7.19 (dd, 1H, J=8.1 Hz and 2.2 Hz, Hm Ar); 6.85 (d, 1H, J=8.1 Hz, Ho Ar); 5.44 (s, 2H, CH2Ar); 2.77 (t, 2H, J=7.6 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.73 (p, 2H, J=7.6 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.25 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.85 (ta, 3H, J=6.7 Hz, CH3). 13C-NMR (CDCl3) δ: 165.5 C3; 152.8 C5; 150.6 Cm pyr; 138.3 Cipso pyr; 135.1 Cipso Ar; 132.8 Cp Ar; 131.9 (Co′ Ar); 129.7 Co Ar; 128.8 Cm′ Ar; 127.8 Cm Ar; 122.3 Co pyr; 50.1 CH2Ar; 31.6 CH2CH2CH2CH2CH2CH2CH3; 29.3 CH2CH2CH2CH2CH2CH2CH3; 29.0 CH2CH2CH2CH2CH2CH2CH3; 28.3CH2CH2CH2CH2CH2CH2CH3; 22.6 CH2CH2CH2CH2CH2CH2CH3; 14.1 CH3. MS (ES+)m/z: 403 (100%) [M+H]+. HPLC: Acetonitrile/H2O 90 : 10, tR=30.3 min (90% purity).


#### 2.1.5. General Procedure for the Synthesis of16–18

To a solution of the corresponding triazole (1 equiv) in dry CH2Cl2 (4–10 mL), excess of MeI was added. The reaction mixture was stirred at room temperature for the time indicated. Afterwards, solvent was removedin vacuo and the residue was purified by chromatography or recrystallization from Et2O/CH2Cl2.4-(1-Benzyl-5-heptyl-1H-1,2,4-triazol-3-yl)-1-methylpyridinium Iodide (
16
). Compound16 was prepared from13a (15 mg, 0.05 mmol) and MeI (4 μL, 0.07 mmol); reaction time: 16 h. Purification: flash chromatography [CH2Cl2/MeOH (99 : 1) → CH2Cl2/MeOH (9 : 1)]. Yield: 14 mg of16 (66%) as a yellow gummy solid. 1H-NMR (CDCl3) δ: 9.18 (d, 2H, J=6.7 Hz, Hm pyr); 8.54 (d, 2H, J=6.7 Hz, Ho pyr); 7.35 (m, 3H, Ph); 7.19 (m, 2H, Ph); 5.36 (s, 2H, CH2Ar); 4.68 (s, 3H, NMe); 2.71 (t, 2H, J=7.6 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.67 (p, 2H, J=7.6 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.23 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.85 (bt, 3H, J=7.0 Hz, CH3). 13C-NMR (CDCl3) δ: 159.0 C3; 155.3 C5; 146.7 Cipso pyr; 145.6 Cm pyr; 134.3 Cipso Ph; 129.1 Cm Ph; 128.6 Cp Ph; 127.3 Co Ph; 123.7 Co pyr; 53.0 CH2Ar; 49.1 NMe; 31.5 CH2CH2CH2CH2CH2CH2CH3; 29.0 CH2CH2CH2CH2CH2CH2CH3; 28.8 CH2CH2CH2CH2CH2CH2CH3; 27.2 CH2CH2CH2CH2CH2CH2CH3; 26.1CH2CH2CH2CH2CH2CH2CH3; 22.5 CH2CH2CH2CH2CH2CH2CH3; 14.0 CH3. MS (ES+)m/z: 349 (100%), [M]+. Anal (C22H29IN4) % calculated (% found) C: 55.47 (55.42); H: 6.14 (6.30); N: 11.76 (11.57).4-[1-(4-Chlorobenzyl)-5-heptyl-1H-1,2,4-triazol-3-yl]-1-methylpyridinium Iodide (
17
). Compound17 was prepared from14a (70 mg, 0.2 mmol) and MeI (140 μL 2.3 mmol); reaction time: 8 days. Purification: flash chromatography [CH2Cl2/MeOH (95 : 5)]. Yield: 87 mg of17 (90%) as a yellow gummy solid. 1H-NMR (CDCl3) δ: 9.21 (d, 2H, J=6.8 Hz, Hm pyr); 8.54 (d, 2H, J=6.8 Hz, Ho pyr); 7.33 (d, 2H, J=8.5 Hz, Hm Ar); 7.15 (d, 2H, J=8.5 Hz, Ho Ar); 5.33 (s, 2H, CH2Ar); 4.68 (s, 3H, NMe); 2.71 (t, 2H, J=7.5 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.69 (p, 2H, J=7.5 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.23 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.85 (bt, 3H, J=6.5 Hz, CH3). 13C-NMR (CDCl3) δ: 158.9 C3; 155.4 C5; 146.3 Cipso pyr; 145.7 Cm pyr; 134.4 Cipso Ar; 132.7 Cp Ar; 129.1 Cm Ar; 128.7 Co Ar; 123.5 Co pyr; 52.1 CH2Ar; 48.9 NMe; 31.4 CH2CH2CH2CH2CH2CH2CH3; 28.9 CH2CH2CH2CH2CH2CH2CH3; 28.6 CH2CH2CH2CH2CH2CH2CH3; 27.0 CH2CH2CH2CH2CH2CH2CH3; 25.9CH2CH2CH2CH2CH2CH2CH3; 22.4 CH2CH2CH2CH2CH2CH2CH3; 13.9 CH3. MS (ES+)m/z: 383 (100%) [M]+. Anal (C22H28ClIN4) % calculated (% found) C: 51.73 (51.58); H: 5.52 (5.41); N: 10.97 (10.72).4-[1-(2,4-Dichlorobenzyl)-5-heptyl-1H-1,2,4-triazol-3-yl]-1-methylpyridinium Iodide (
18
). Compound18 was prepared from15a (50 mg, 0.1 mmol) and MeI (277 μL 4.45 mmol); reaction time: 8 days. Purification: recrystallization from Et2O/CH2Cl2. Yield: 43 mg of18 (64%) as a yellow solid. Mp = 136–138°C. 1H-NMR (CDCl3) δ: 9.22 (d, 2H, J=6.7 Hz, Hm pyr); 8.53 (d, 2H, J=6.7 Hz, Ho pyr); 7.44 (d, 1H, J=1.9 Hz, Hm′ Ar); 7.25 (dd, 1H, J=8.4 Hz and 1.9 Hz, Hm Ar); 7.00 (d, 1H, J=8.4 Hz, Ho Ar); 5.42 (s, 2H, CH2Ar); 4.69 (s, 3H, NMe); 2.76 (t, 2H, J=7.6 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.72 (p, 2H, J=7.6 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.26 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.86 (bt, 3H, J=7.3 Hz, CH3). 13C-NMR (CDCl3) δ: 159.4 C3; 155.8 C5; 146.5 Cipso pyr; 145.7 Cm pyr; 135.4 Cipso Ar; 133.4 Cp Ar; 130.6 Co′ Ar; 130.3 Co Ar; 129.7 Cm′ Ar; 128,0 (Cm BnAr); 123,7 (Co CAr); 49,5 (CH2Ar); 49,2 (NMe); 31.5 CH2CH2CH2CH2CH2CH2CH3; 29.0 CH2CH2CH2CH2CH2CH2CH3; 28.8 CH2CH2CH2CH2CH2CH2CH3; 27.3 CH2CH2CH2CH2CH2CH2CH3; 25.9CH2CH2CH2CH2CH2CH2CH3; 22.5 CH2CH2CH2CH2CH2CH2CH3; 14.0 CH3. MS (ES+)m/z: 417 (100%) [M]+. Anal (C22H27Cl2IN4) % calculated (% found) C: 48.46 (48.71); H: 4.99 (5.20); N: 10.27 (10.06).


### 2.2. Pharmacology

Radioligand Binding Assays. CB1R binding assays in rat cerebellar membranes were performed using [3H]-SR141716A and [3H]-WIN552122 (NEN-Dupont, Boston, MA, 40–60 Ci/mmol) as radioligands, using the previously described methods [14]. Ki were calculated from the equation of Yung-Chi and Prusoff [15], using fixed Kd values for either [3H]-WIN552122 (8 nM) or [3H]-SR141716A (4 nM) obtained from independent experimental assays.


## 2.1. Chemistry

### 2.1.1. General

All reagents and solvents were used as commercially received. EtOH was dried over magnesium. TLC was carried out by precoated silica-gel 60 F254 plates (Merck) and detection by UV light (254 nm). Flash-column chromatography was carried out by Kieselgel 60 (230–400 mesh; Merck). Medium pressure chromatography (MPLC) was carried out by Flash Master Personal system with prepacked silica-gel cartridges. The purity of the final compounds was determined by elemental analysis or analytical HPLC. Elemental analysis was performed on a Heraeus CHN-O rapid analyzer. Analyses indicated by the symbols of the elements or functions were within ±0.4% of the theoretical values, except compound6. Analytical HPLC was run on a Waters 6000 with Delta Pak C 18.5 mm, 300 Ǻ (3.9 × 150 mm) column, using an eluent Acetonitrile/H2O (0.05% H3PO4 + 0.04% TEA) in the proportion indicated in each case; flow rate used was 1 mL/min and the UV absorption was detected at a wavelength of 254 nm. HPLC analyses were within ≥90% of purity, except compound11b (81% purity). The mass spectra (electrospray positive mode) were determined on a MSD-Series 1100 Hewlett Packard instrument. Melting points (uncorrected) were determined with a Reichert Jung Thermovar apparatus. 1H and 13C NMR spectra were recorded on a Gemini 200, Varian 300 and 400 unity spectrometers using TMS as the internal standard. All chemical shifts are reported in ppm. For the assignment of the protons and carbons of the aromatic rings Scheme 1 is used.Scheme 1


### 2.1.2. General Procedure for the Synthesis of1 and2

To a suspension of the corresponding nitrile (10 equiv) in dry EtOH (30–75 mL) NaOMe (1 equiv) was added. It was stirred at room temperature under N2 atmosphere for 48 h. Afterwards, ammonium chloride (10 equiv) was added, and the stirring was maintained for 24 more hours. Then, unreacted ammonium chloride was filtered off and the solvent was evaporated from the liquid layer. The white solid obtained was washed with Et2O, dried, and used in the next step without further purification.4-Chlorobenzimidamide Hydrochloride (
1
). Compound1 was prepared from 4-chlorobenzonitrile (10.00 g, 72.7 mmol), NaOMe (393 mg, 7.3 mmol), and ammonium chloride (3.90 g, 72.7 mmol). Yield: 4.34 g of1 (31%) as a white solid. Mp = 246-247°C (236–240°C (EtOH)). [13] 1H-NMR (CD3OD) δ: 8.02 (d, 2H, J=9.0 Hz, Ho); 7.84 (d, 2H, J=9.0 Hz, Hm). 13C-NMR (CD3OD) δ: 167.6 C=NH; 141.4 Cp; 130.8 and 130.7 Co and Cm; 128.2 Cipso. MS (ES+)m/z: 155 (100%) [M+H]+.4-Amidinopyridinium Hydrochloride (
2
). Compound2 was prepared from 4-cyanopyridine (2.50 g, 24.0 mmol), NaOMe (130 mg, 2.4 mmol), and ammonium chloride (1.28 g, 24.0 mmol). Yield: 3.30 g of2 (87%) as a white solid. Mp = 248-249°C. 1H-NMR (CD3OD) δ: 8.93 (d, 2H, J=6.2 Hz, Hm); 7.87 (d, 2H, J=6.2 Hz, Ho). 13C-NMR (CD3OD) δ: 166.9 C=NH; 151.7 Cm; 138.1 Cipso; 123.2 Co. MS (ES+)m/z: 122 (100%) [M+H]+.


### 2.1.3. General Procedure for the Synthesis of3–5

To a solution of the corresponding amidinium salt (1.5 equiv) in dry EtOH (10–45 mL), NaOMe (1 equiv) in 10 mL of dry EtOH was added. The suspension was stirred at room temperature for 1 h. Then, the solid formed was filtered on Celite. Octanoic hydrazide (2 equiv) was added to the liquid layer and the mixture was stirred under reflux for 46–49 h. After cooling the reaction mixture, solvent was removedin vacuo. The residue was dissolved in CH2Cl2 and washed with water (3 × 20 mL). The organic layer was dried over anhydrous Na2SO4 and the solvent was removedin vacuo. The obtained residue was purified by MPLC using cyclohexane/EtOAc (3 : 1) as eluent, except for compound5 where cyclohexane/EtOAc (3 : 1 to 4 : 1) was used.3-Heptyl-5-phenyl-1H-1,2,4-triazole (
3
). Compound3 was prepared from benzamidine hydrochloride hydrate (857 mg, 5.5 mmol), octanoic hydrazide (581 mg, 3.6 mmol), and NaOMe (394 mg, 7.3 mmol). Yield: 683 mg of3 (78%) as a transparent oil. Mp = 129–132°C oxalate (to a solution of the free base in Et2O, a solution of oxalic acid in EtOAc was added; the white solid was filtered off, washed with EtOAc, and dried). 1H-NMR (CDCl3) δ: 10.65 (bs, 1H, NH); 7.96 (m, 2H, Ho); 7.34 (m, 3H, Hm and Hp); 2.69 (t, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.65 (p, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.16 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.79 (bt, 3H, J=6.3 Hz, CH3). 13C-NMR (CDCl3) δ: 160.4 and 160.0 C3 and C5; 129.8 Cipso; 129.5 Cp; 128.6 Cm; 126.4 Co; 31.5 CH2CH2CH2CH2CH2CH2CH3; 29.1 CH2CH2CH2CH2CH2CH2CH3; 28.8 CH2CH2CH2CH2CH2CH2CH3; 28.1 CH2CH2CH2CH2CH2CH2CH3; 27.0CH2CH2CH2CH2CH2CH2CH3; 22.5 CH2CH2CH2CH2CH2CH2CH3; 13.9 CH3. MS (ES+)m/z: 244 (100%) [M+H]+. Anal (C15H21N3·C2H2O4) % calculated (% found) C: 61.25 (61.41); H: 6.95 (7.12); N: 12.60 (12.62).5-(4-Chlorophenyl)-3-heptyl-1H-1,2,4-triazole (
4
). Compound4 was prepared from1 (1.00 g, 5.2 mmol), octanoic hydrazide (549 mg, 3.5 mmol), and NaOMe (375 mg, 7.0 mmol). Yield 392 mg of4 (40%) as a white solid. Mp = 108–111°C. 1H-NMR (CDCl3) δ: 7.91 (d, 2H, J=8.6 Hz, Ho); 7.34 (d, 2H, J=8.6 Hz, Hm); 2.72 (t, 2H, J=7.6 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.68 (p, 2H, J=7.6 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.20 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.82 (bt, 3H, J=6.5 Hz, CH3). 13C-NMR (CDCl3) δ: 160.4 and 159.5 C3 and C5; 135.5 Cipso; 128.9 Cm; 128.7 Cp; 127.7 Co; 31.6 CH2CH2CH2CH2CH2CH2CH3; 29.2 CH2CH2CH2CH2CH2CH2CH3; 28.8 CH2CH2CH2CH2CH2CH2CH3; 28.0 CH2CH2CH2CH2CH2CH2CH3; 26.9CH2CH2CH2CH2CH2CH2CH3; 22.5 CH2CH2CH2CH2CH2CH2CH3; 14.0 CH3. MS (ES+)m/z: 278 (100%) [M+H]+. Anal (C15H20ClN3) % calculated (% found) C: 64.85 (65.09); H: 7.26 (7.42); N: 15.13 (15.35).4-(3-Heptyl-1H-1,2,4-triazol-5-yl)pyridine (
5
) and N′-[imino(pyridin-4-yl)methyl]octa-nehydrazide (6). Compound5 was prepared from2 (2.00 g, 12.7 mmol), octanoic hydrazide (1.35 g, 8.5 mmol), and NaOMe (918 mg, 17.0 mmol). Yield: 459 mg of5 (23%) as a white solid and 1.61 g of6 (45%) as a white solid.5: Mp = 109–112°C. 1H-NMR (CDCl3) δ: 8.69 (d, 2H, J=6.1 Hz, Hm); 8.06 (d, 2H, J=6.1 Hz, Ho); 2.85 (t, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.78 (p, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.21 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.81 (bt, 3H, J=6.7 Hz, CH3). 13C-NMR (CDCl3) δ: 159.4 and 159.2 C3 and C5; 149.4 Cm; 139.5 Cipso; 121.0 Co; 31.5 CH2CH2CH2CH2CH2CH2CH3; 29.1 CH2CH2CH2CH2CH2CH2CH3; 28.8 CH2CH2CH2CH2CH2CH2CH3; 28.1 CH2CH2CH2CH2CH2CH2CH3; 26.7CH2CH2CH2CH2CH2CH2CH3; 22.5 CH2CH2CH2CH2CH2CH2CH3; 13.9 CH3. MS (ES+)m/z: 245 (100%) [M+H]+. Anal (C14H20N4) % calculated (% found) C: 68.82 (68.71); H: 8.25 (8.36); N: 22.93 (22.78).6: Mp = 135–138°C. 1H-NMR (CD3OD) δ: 8.67 (d, 2H, J=6.1 Hz, Hm); 7.94 (d, 2H, J=6.1 Hz, Ho); 2.41 (t, 2H, J=7.4 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.76 (m, 2H, CH2CH2CH2CH2CH2CH2CH3); 1.41 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0,99 (bt, 3H, J=6.0 Hz, CH3). 13C-NMR (CD3OD) δ: 172.7 CONH; 151.7 C=NH; 150.4 Cm; 144.1 Cipso; 122.9 Co; 35.7CH2CH2CH2CH2CH2CH2CH3; 32.9 CH2CH2CH2CH2CH2CH2CH3; 30.4 and 30.2 CH2CH2CH2CH2CH2CH2CH3; 27.1 CH2CH2CH2CH2CH2CH2CH3; 23.7 CH2CH2CH2CH2CH2CH2CH3; 14.4 CH3. MS (ES+)m/z: 263 (100%) [M+H]+. Anal (C14H22N4O·1/2HCl) % calculated (% found) C: 59.93 (59.10); H: 8.08 (8.11); N: 19.97 (20.45).Intermediate6 (1.00 g, 3.6 mmol) in dry EtOH (20 mL) reacted by refluxing with NaOMe (1.03 g, 19.0 mmol) for 4 days. Under this procedure,5 was obtained in 78% yield (676 mg).


### 2.1.4. General Procedure for the Synthesis of7a–15a and7b–15b

To a solution of the 3,5-disubstituted triazole (1 equiv) in 40% NaOH aq solution (3–5 mL) and toluene (7–10 mL) (Bu)4NBr (0.05 equiv) were first added, and later the corresponding alkylating agent (1 equiv) was added. The reaction mixture was stirred at 80–90°C (bath temperature) for the reaction time indicated. Afterwards, organic layer was separated and the aqueous layer was extracted with CH2Cl2 (3 × 25 mL). The combined organic layers were dried over anhydrous Na2SO4 and the solvent was removedin vacuo. The residue was purified by MPLC [cyclohexane/EtOAc (9 : 1)], except for compound13a, which was purified by flash chromatography [CH2Cl2 → CH2Cl2/MeOH (60 : 1)].1-Benzyl-5-heptyl-3-phenyl-1H-1,2,4-triazole (
7a
) and 1-Benzyl-3-heptyl-5-phenyl-1H-1,2,4-triazole (
7b
). Compounds7a and7b were prepared from3 (100 mg, 0.4 mmol), benzyl bromide (52 μL, 0.4 mmol), and (Bu)4NBr (6 mg, 0.02 mmol); reaction time: 1.5 h. Yield: 106 mg of7a (78%) as a transparent oil, and 13 mg of7b (9%) as a transparent oil.7a: 1H-NMR (CDCl3) δ: 8.08 (m, 2H, Ph); 7.40 (m, 3H, Ph); 7.31 (m, 3H, Bn); 7.18 (m, 2H, Bn); 5.34 (s, 2H, CH2Ph); 2.69 (t, 2H, J=7.8 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.64 (p, 2H, J=7.5 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.22 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.85 (bt, 3H, J=6.1 Hz, CH3). 13C-NMR (CDCl3) δ: 160.8 C3; 157.0 C5; 135.7 Cipso Bn; 131.2 Cipso Ph; 128.9 Cm Bn and Cp Ph; 128.5 Cm Ph; 126.9 Co Bn; 126.3 Co Ph; 128.1 Cp Bn; 52.1 (CH2Ph); 31.6 CH2CH2CH2CH2CH2CH2CH3; 29.2 CH2CH2CH2CH2CH2CH2CH3; 28.8 CH2CH2CH2CH2CH2CH2CH3; 27.8 CH2CH2CH2CH2CH2CH2CH3; 26.2CH2CH2CH2CH2CH2CH2CH3; 22.5 CH2CH2CH2CH2CH2CH2CH3; 14.0 CH3. MS (ES+)m/z: 334 (100%) [M+H]+. Anal (C22H27N3) % calculated (% found) C: 79.24 (79.35); H: 8.16 (8.40); N: 12.60 (12.64).7b: 1H-NMR (CDCl3) δ: 7.53 (m, 2H, Ph); 7.41 (m, 3H, Ph); 7.30 (m, 3H, Bn); 7.14 (m, 2H, Bn); 5.35 (s, 2H, CH2Ph); 2.75 (t, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.78 (p, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.23 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.85 (bt, 3H, J=6.4 Hz, CH3). 13C-NMR (CDCl3) δ: 164.1 C3; 155.3 C5; 136.1 Cipso Bn; 130.2 Cipso Ph; 128.9 Cm Bn; 128.8 and 128.7 Co and Cm Ph; 126.7 Co Bn; 127.9 Cp Bn and Cp Ph; 52.4 CH2Ph; 31.8 CH2CH2CH2CH2CH2CH2CH3; 29.3 CH2CH2CH2CH2CH2CH2CH3; 29.0 CH2CH2CH2CH2CH2CH2CH3; 28.5 CH2CH2CH2CH2CH2CH2CH3; 28.3CH2CH2CH2CH2CH2CH2CH3; 22.6 CH2CH2CH2CH2CH2CH2CH3; 14.1 CH3. MS (ES+)m/z: 334 (100%) [M+H]+. Anal (C22H27N3) % calculated (% found) C: 79.24 (79.10); H: 8.16 (7.95); N: 12.60 (12.42).1-(4-Chlorobenzyl)-5-heptyl-3-phenyl-1H-1,2,4-triazole (
8a
) and 1-(4-Chlorobenzyl)-3-heptyl-5-phenyl-1H-1,2,4-triazole (
8b
). Compounds8a and8b were prepared from3 (73 mg, 0.3 mmol), 4-chlorobenzyl chloride (48 mg, 0.3 mmol), and (Bu)4NBr (6 mg, 0.02 mmol); reaction time: 11 h. Yield: 96 mg of8a (87%) as a yellow solid and 10 mg of8b (9%) as a transparent oil.8a: 1H-NMR (CDCl3) δ: 8.07 (m, 2H, Ho Ph); 7.37 (m, 3H, Hm and Hp Ph); 7.29 (d, 2H, J=8.4 Hz, Hm Bn); 7.11 (d, 2H, J=8.4 Hz, Ho Bn); 5.27 (s, 2H, CH2Ar); 2.67 (t, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.69 (p, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.24 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.85 (ta, 3H, J=6.9 Hz, CH3). 13C-NMR (CDCl3) δ: 161.0 C3; 156.9 C5; 134.2 Cipso Bn; 134.0 Cp Bn; 131.1 Cipso Ph; 129.0 and 128.3 Co Bn, Cm Bn and Cp Ph; 128.5 Cm Ph; 126.2 Co Ph; 51.3 CH2Ar; 31.5 CH2CH2CH2CH2CH2CH2CH3; 29.2 CH2CH2CH2CH2CH2CH2CH3; 28.8 CH2CH2CH2CH2CH2CH2CH3; 27.7 CH2CH2CH2CH2CH2CH2CH3; 26.1CH2CH2CH2CH2CH2CH2CH3; 22.5 CH2CH2CH2CH2CH2CH2CH3; 14.0 CH3. MS (ES+)m/z: 368 (100%) [M+H]+. Anal (C22H26ClN3) % calculated (% found) C: 71.82 (71.75); H: 7.12 (6.98); N: 11.42 (11.63).8b: 1H-NMR (CDCl3) δ: 7.50 (m, 2H, H Ph); 7.43 (m, 3H, Ph); 7.29 (d, 2H, J=8.4 Hz, Hm Bn); 7.06 (d, 2H, J=8.4 Hz, Ho Bn); 5.30 (s, 2H, CH2Ar); 2.75 (t, 2H, J=7.5 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.77 (p, 2H, J=7.5 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.23 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.85 (ta, 3H, J=6.2 Hz, CH3). 13C-NMR (CDCl3) δ: 164.6 C3; 155.5 C5; 134.7 Cipso Bn; 133.8 Cp Bn; 130.1 Cp Ph; 129.0 Cm Bn; 128.8 Co Bn; 128.6 Cm Ph; 128.1 Co Ph; 128.0 Cipso Ph; 51.7 CH2Ar; 31.8 CH2CH2CH2CH2CH2CH2CH3; 29.4 CH2CH2CH2CH2CH2CH2CH3; 29.0 CH2CH2CH2CH2CH2CH2CH3; 28.5CH2CH2CH2CH2CH2CH2CH3; 22.6 CH2CH2CH2CH2CH2CH2CH3; 14.1 CH3. MS (ES+)m/z: 368 (100%) [M+H]+. HPLC: Acetonitrile/H2O 95 : 5, tR=28.5 min (99% purity).1-(2,4-Dichlorobenzyl)-5-heptyl-3-phenyl-1H-1,2,4-triazole (
9a
) and 1-(2,4-Dichloro-benzyl)-3-heptyl-5-phenyl-1H-1,2,4-triazole (
9b
). Compounds9a and9b were prepared from3 (100 mg, 0.4 mmol), 2,4-dichlorobenzyl chloride (57 μL, 0.4 mmol), and (Bu)4NBr (6 mg, 0.02 mmol); reaction time: 5 h. Yield: 123 mg of9a (74%) as a white solid and 12 mg of9b (7%) as a transparent oil.9a: Mp = 70–73°C. 1H-NMR (CDCl3) δ: 8.11 (m, 2H, Ho Ph); 7.42 (m, 4H, Hm′ Bn, Hm and Hp Ph); 7.19 (dd, 1H,J = 8.3 Hz and 1.6 Hz, Hm Ph); 6.85 (d, 1H, J=8.3 Hz, Ho Bn); 5.41 (s, 2H, CH2Ar); 2.73 (t, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.73 (p, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.27 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.88 (bt, 3H, J=6.4 Hz, CH3). 13C-NMR (CDCl3) δ: 161.4 C3; 157.4 C5; 134.5 Cipso Bn; 132.8 Cp Bn; 132.2 Co′ Bn; 131.0 Cipso Ph; 129.3 Co Bn; 129.2 Cm′ Bn; 129.1 Cp Ph; 128.5 Cm Ph; 127.7 Cm Bn; 126.3 Co Ph; 48.6 CH2Ar; 31.5 CH2CH2CH2CH2CH2CH2CH3; 29.1 CH2CH2CH2CH2CH2CH2CH3; 28.8 CH2CH2CH2CH2CH2CH2CH3; 27.8 CH2CH2CH2CH2CH2CH2CH3; 26.0CH2CH2CH2CH2CH2CH2CH3; 22.5 CH2CH2CH2CH2CH2CH2CH3; 14.0 CH3. MS (ES+)m/z: 402 (100%) [M+H]+. Anal (C22H25Cl2N3) % calculated (% found) C: 65.67 (65.42); H: 6.26 (6.50); N: 10.44 (10.35).9b: 1H-NMR (CDCl3) δ: 7.50–7.43 (m, 5H, Ph); 7.40 (d, 1H, J=1.7 Hz, Hm′ Bn); 7.20 (dd, 1H, J=8.6 Hz and 1.7 Hz, Hm Bn); 6.84 (d, 1H, J=8.6 Hz, Ho Bn); 5.39 (s, 2H, CH2Ar); 2.76 (t, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.79 (p, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.23 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.85 (bt, 3H, J=6.1 Hz, CH3). 13C-NMR (CDCl3) δ: 165.2 C3; 156.2 C5; 134.6 Cipso Bn; 133.0 Cp Bn; 132.9 Co′ Bn; 130.5 Co Bn; 129.7 Cm′ Bn; 129.2 Cm Ph; 129.0 Cp Ph; 128.6 Co Ph; 127.9 Cm Bn; 127.8 Cipso Ph; 50.1 CH2Ar; 32.0 CH2CH2CH2CH2CH2CH2CH3; 29.6 CH2CH2CH2CH2CH2CH2CH3; 29.2 CH2CH2CH2CH2CH2CH2CH3; 28.7CH2CH2CH2CH2CH2CH2CH3; 22.9 CH2CH2CH2CH2CH2CH2CH3; 14.3 CH3. MS (ES+)m/z: 402 (100%) [M+H]+. Anal (C22H25Cl2N3·C6H12) % calculated (% found) C: 69.12 (69.42); H: 7.67 (7.79); N: 8.64 (8.24).1-Benzyl-3-(4-chlorophenyl)-5-heptyl-1H-1,2,4-triazole (
10a
) and 1-Benzyl-5-(4-chlo-rophenyl)-3-heptyl-1H-1,2,4-triazole (
10b
). Compounds10a and10b were prepared from4 (80 mg, 0.3 mmol), benzyl bromide (34 μL, 0.3 mmol), and (Bu)4NBr (6 mg, 0.02 mmol); reaction time: 20 min. Yield: 94 mg of10a (89%) as a white solid and 9 mg of10b (8%) as a yellow oil.10a: Mp = 47–50°C. 1H-NMR (CDCl3) δ: 8.02 (d, 2H, J=8.8 Hz, Ho Ar); 7.36 (d, 2H, J=8.8 Hz, Hm Ar); 7.29 (m, 3H, Bn); 7.19 (m, 2H, Bn); 5.31 (s, 2H, CH2Ph); 2.67 (t, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.66 (p, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.22 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.85 (bt, 3H, J=6.9 Hz, CH3). 13C-NMR (CDCl3) δ: 159.9 C3; 157.1 C5; 135.5 Cipso Ar; 134.7 Cipso Bn; 129.8 Cp Ar; 128.9 Cm Ar; 128.6 Cm Bn; 128.1 Cp Bn; 127.6 Co Ar; 126.9 Co Bn; 52.1 (CH2Ph); 31.5 CH2CH2CH2CH2CH2CH2CH3; 29.2 CH2CH2CH2CH2CH2CH2CH3, 28.8 CH2CH2CH2CH2CH2CH2CH3; 27.7 CH2CH2CH2CH2CH2CH2CH3; 26.1CH2CH2CH2CH2CH2CH2CH3; 22.5 CH2CH2CH2CH2CH2CH2CH3; 14.0 CH3. MS (ES+)m/z: 368 (100%) [M+H]+. Anal (C22H26ClN3) % calculated (% found) C: 71.82 (72.02); H: 7.12 (6.89); N: 11.42 (11.24).10b: 1H-NMR (CDCl3) δ: 7.47 (d, 2H, J=8.5 Hz, Hm Ar); 7.38 (d, 2H, J=8.5 Hz, Ho Ar); 7.30 (m, 3H, Bn); 7.00 (m, 2H, Bn); 5.33 (s, 2H, CH2Ph); 2.75 (t, 2H, J=7.6 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.78 (p, 2H, J=7.6 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.25 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.85 (bt, 3H, J=6.4 Hz, CH3). 13C-NMR (CDCl3) δ: 164.6 C3; 155.0 C5; 136.3 Cipso Ar; 136.2 Cipso Bn; 130.0 Cm Ar; 129.4 Cp Ar; 129.1 Co Ar; 129.0 Cm Bn; 128.0 Cp Bn; 52.6 (CH2Ph); 31.8 CH2CH2CH2CH2CH2CH2CH3; 29.4 CH2CH2CH2CH2CH2CH2CH3; 29.0 CH2CH2CH2CH2CH2CH2CH3; 28.5CH2CH2CH2CH2CH2CH2CH3; 22.6 CH2CH2CH2CH2CH2CH2CH3; 14.0 CH3. MS (ES+)m/z: 368 (100%) [M+H]+.1-(4-Chlorobenzyl)-3-(4-chlorophenyl)-5-heptyl-1H-1,2,4-triazole (
11a
) and 1-(4-Chlo-robenzyl)-5-(4-chlorophenyl)-3-heptyl-1H-1,2,4-triazole (
11b
). Compounds11a and11b were prepared from4 (100 mg, 0.4 mmol), 4-chlorobenzyl chloride (64 mg, 0.4 mmol), and (Bu)4NBr (6 mg, 0.02 mmol); reaction time: 6 h. Yield: 132 mg of11a (91%) as a white solid and 2 mg of11b (1%) as a transparent oil.11a: Mp = 58–60°C. 1H-NMR (CDCl3) δ: 8.00 (d, 2H, J=8.8 Hz, Ho Ar); 7.35 (d, 2H, J=8.8 Hz, Hm Ar); 7.28 (d, 2H, J=8.4 Hz, Hm CH2Ar); 7.10 (d, 2H, J=8.4 Hz, Ho CH2Ar); 5.26 (s, 2H, CH2Ar); 2.66 (t, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.66 (p, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.23 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.84 (bt, 3H, J=6.3 Hz, CH3). 13C-NMR (CDCl3) δ: 160.1 C3; 157.1 C5; 134.8 Cipso Ar; 134.1 Cipso CH2Ar; 134.0 Cp CH2Ar; 129.6 Cp Ar; 129.0 Cm Ar; 128.6 Cm CH2Ar; 128.3 Co CH2Ar; 127.5 Co Ar; 51.3 (CH2Ar); 31.5 CH2CH2CH2CH2CH2CH2CH3; 29.2 CH2CH2CH2CH2CH2CH2CH3; 28.8 CH2CH2CH2CH2CH2CH2CH3; 27.7 CH2CH2CH2CH2CH2CH2CH3; 26.1CH2CH2CH2CH2CH2CH2CH3; 22.5 CH2CH2CH2CH2CH2CH2CH3; 14.0 CH3. MS (ES+)m/z: 402 (100%) [M+H]+. Anal (C22H25Cl2N3) % calculated (% found) C: 65.67 (65.72); H: 6.26 (6.12); N: 10.44 (10.40).11b: 1H-NMR (CDCl3) δ: 7.45 (d, 2H, J=9.0 Hz, Ho Ar); 7.39 (d, 2H, J=9.0 Hz, Hm Ar); 7.30 (d, 2H, J=8.3 Hz, Hm CH2Ar); 7.05 (d, 2H, J=8.3 Hz, Ho CH2Ar); 5.29 (s, 2H, CH2Ar); 2.70 (t, 2H, J=7.8 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.75 (p, 2H, J=7.8 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.23 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.82 (bt, 3H, J=6.2 Hz, CH3). 13C-NMR (CDCl3) δ: 164.7 C3; 154.4 C5; 136.4 Cipso Ar; 134.4 Cipso CH2Ar; 134.0 Cp CH2Ar; 129.9 Cm and Cp Ar; 129.2 Co Ar and Cm CH2Ar; 128.0 Co CH2Ar; 51.8 CH2Ar; 31.8 CH2CH2CH2CH2CH2CH2CH3; 29.7 CH2CH2CH2CH2CH2CH2CH3; 29.3 CH2CH2CH2CH2CH2CH2CH3; 29.0 CH2CH2CH2CH2CH2CH2CH3; 28.4CH2CH2CH2CH2CH2CH2CH3; 22.6 CH2CH2CH2CH2CH2CH2CH3; 14.1 CH3. MS (ES+)m/z: 402 (100%) [M+H]+. HPLC: Acetonitrile/H2O 95 : 5, tR=25.5 min (81% purity).3-(4-Chlorophenyl)-1-(2,4-dichlorobenzyl)-5-heptyl-1H-1,2,4-triazole (
12a
) and 5-(4-Chlorophenyl)-1-(2,4-dichlorobenzyl)-3-heptyl-1H-1,2,4-triazole (
12b
). Compounds12a and12b were prepared from4 (90 mg, 0.3 mmol), 2,4-dichlorobenzyl chloride (45 μL, 0.3 mmol), and (Bu)4NBr (6 mg, 0.02 mmol); reaction time: 6 h. Yield: 122 mg of12a (86%) as a white solid and 8 mg of12b (6%) as a transparent oil.12a: Mp = 97–99°C. 1H-NMR (CDCl3) δ: 8.00 (d, 2H, J=8.6 Hz, Ho Ar); 7.41 (d, 1H, J=2.0 Hz, Hm′ CH2Ar); 7.37 (d, 2H, J=8.6 Hz, Hm Ar); 7.18 (dd, 1H, J=8.4 Hz and 2.0 Hz, Hm CH2Ar); 6.84 (d, 1H, J=8.4 Hz, Ho CH2Ar); 5.37 (s, 2H, CH2Ar); 2.70 (t, 2H, J=7.6 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.69 (p, 2H, J=7.6 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.22 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.85 (bt, 3H, J=7.1 Hz, CH3). 13C-NMR (CDCl3) δ: 160.5 C3; 157.6 C5; 135.0 Cipso Ar; 134.6 Cipso CH2Ar; 132.9 Cp CH2Ar; 132.0 Co′ CH2Ar; 129.5 Cp Ar; 129.4 Co CH2Ar; 129.3 Cm′ CH2Ar; 128.7 Cm Ar; 127.7 Cm CH2Ar; 127.6 Co Ar; 48.7 CH2Ar; 31.5 CH2CH2CH2CH2CH2CH2CH3; 29.1 CH2CH2CH2CH2CH2CH2CH3; 28.8 CH2CH2CH2CH2CH2CH2CH3; 27.7 CH2CH2CH2CH2CH2CH2CH3; 26.0CH2CH2CH2CH2CH2CH2CH3; 22.5 CH2CH2CH2CH2CH2CH2CH3; 14.0 CH3. MS (ES+)m/z: 436 (100%) [M+H]+. Anal (C22H24Cl3N3) % calculated (% found) C: 60.49 (60.42); H: 5.54 (5.74); N: 9.62 (9.42).12b: 1H-NMR (CDCl3) δ: 7.41 (bs, 5H, Ho Ar, Hm Ar and Hm′ CH2Ar); 7.21 (dd, 1H, J=8.3 Hz and 2.0 Hz, Hm CH2Ar); 6.84 (d, 1H, J=8.3 Hz, Ho CH2Ar); 5.37 (s, 2H, CH2Ar); 2.75 (t, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.77 (p, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1,23 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.85 (bt, 3H, J=6.1 Hz, CH3). 13C-NMR (CDCl3) δ: 165.1 C3; 154.9 C5; 136.6 Cipso Ar; 134.7 Cipso CH2Ar; 132.8 Cp CH2Ar; 132.5 Co′ CH2Ar; 129.7 Cm and Cp Ar; 129.6 Co CH2Ar; 129.3 Co Ar; 129.0 Cm′ CH2Ar; 127.8 Cm CH2Ar; 49.9 CH2Ar; 31.8 CH2CH2CH2CH2CH2CH2CH3; 29.7 CH2CH2CH2CH2CH2CH2CH3; 29.3 CH2CH2CH2CH2CH2CH2CH3; 29.0 CH2CH2CH2CH2CH2CH2CH3; 28.4CH2CH2CH2CH2CH2CH2CH3; 22.6 CH2CH2CH2CH2CH2CH2CH3; 14.0 CH3. MS (ES+)m/z: 436 (99%) [M+H]+. HPLC: Acetonitrile/H2O 90 : 10, tR=66.2 min (99% purity).4-(1-Benzyl-5-heptyl-1H-1,2,4-triazol-3-yl)pyridine (
13a
). Compound13a was prepared from5 (150 mg, 0.6 mmol), benzyl chloride (73 μL, 0.6 mmol), and (Bu)4NBr (6 mg, 0.02 mmol); reaction time: 2.5 h. Yield: 166 mg of13a (81%) as an orange oil. 1H-NMR (CDCl3) δ: 8.63 (d, 2H, J=6.0 Hz, Hm pyr); 7.93 (d, 2H, J=6.0 Hz, Ho pyr); 7.31 (m, 3H, Ph); 7.16 (m, 2H, Ph); 5.33 (s, 2H, CH2Ar); 2.68 (t, 2H, J=7.8 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.65 (p, 2H, J=7.0 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.21 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.83 (bt, 3H, J=7.1 Hz, CH3). 13C-NMR (CDCl3) δ: 158.7 C3; 157.5 C5; 150.1 Cm pyr; 138.5 Cipso pyr; 135.2 Cipso Ph; 128.9 Cm Ph; 128.2 Cp Ph; 127.0 Co Ph; 120.4 Co pyr; 52.3 CH2Ph; 31.5 CH2CH2CH2CH2CH2CH2CH3; 29.1 CH2CH2CH2CH2CH2CH2CH3; 28.8 CH2CH2CH2CH2CH2CH2CH3; 27.6 CH2CH2CH2CH2CH2CH2CH3; 26.1CH2CH2CH2CH2CH2CH2CH3; 22.5 CH2CH2CH2CH2CH2CH2CH3; 14.0 CH3. MS (ES+)m/z: 335 (100%) [M+H]+. Anal (C21H26N4) % calculated (% found) C: 75.41 (75.71); H: 7.84 (7.79); N: 16.75 (16.54).4-[1-(4-Chlorobenzyl)-5-heptyl-1H-1,2,4-triazol-3-yl]pyridine (
14a
) and 4-[1-(4-Chlo-robenzyl)-3-heptyl-1H-1,2,4-triazol-5-yl]pyridine (
14b
). Compounds14a and14b were prepared from5 (180 mg, 0.7 mmol), 4-chlorobenzyl chloride (119 mg, 0.7 mmol), and (Bu)4NBr (12 mg, 0.04 mmol); reaction time: 6 h. Yield: 150 mg of14a (51%) as a brown solid and 10 mg of14b (4%) as a brown oil.14a: Mp = 77–80°C. 1H-NMR (CDCl3) δ: 8.61 (d, 2H, J=6.0 Hz, Hm pyr); 7.90 (d, 2H, J=6.0 Hz, Ho pyr); 7.27 (d, 2H, J=8.6 Hz, Hm Ar); 7.09 (d, 2H, J=8.6 Hz, Ho Ar); 5.27 (s, 2H, CH2Ar); 2.66 (t, 2H, J=7.8 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.65 (p, 2H, J=7.8 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.20 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.82 (bt, 3H, J=6.6 Hz, CH3). 13C-NMR (CDCl3) δ: 158.9 C3; 157.5 C5; 150.2 Cm pyr; 138.4 Cipso pyr; 134.2 Cipso Ar; 133.7 Cp Ar; 129.1 Cm Ar; 128.4 Co Ar; 120.4 Co pyr; 51.8 CH2Ar; 31.5 CH2CH2CH2CH2CH2CH2CH3; 29.1 CH2CH2CH2CH2CH2CH2CH3; 28.8 CH2CH2CH2CH2CH2CH2CH3; 26.0CH2CH2CH2CH2CH2CH2CH3; 22.5 CH2CH2CH2CH2CH2CH2CH3; 14.0 CH3. MS (ES+)m/z: 369 (100%) [M+H]+. Anal (C21H25ClN4) % calculated (% found) C: 68.37 (68.55); H: 6.83 (6.90); N: 15.19 (15.21).14b: 1H-NMR (CDCl3) δ: 8.65 (d, 2H, J=6.2 Hz, Hm pyr); 7.44 (d, 2H, J=6.2 Hz, Ho pyr); 7.31 (d, 2H, J=8.5 Hz, Hm Ar); 7.06 (d, 2H, J=8.5 Hz, Ho pyr); 5.35 (s, 2H, CH2Ar); 2.76 (t, 2H, J=7.6 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.74 (p, 2H, J=7.6 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.27 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.85 (m, 3H, CH3). 13C-NMR (CDCl3) δ: 165.1 C3; 152.8 C5; 150.5 Cm pyr; 135.4 Cipso pyr; 134.2 Cipso Ar; 134.0 Cp Ar; 129.3 Cm Ar; 128.0 Co Ar; 122.5 Co pyr; 52.1 CH2Ar; 31.7 CH2CH2CH2CH2CH2CH2CH3; 29.3 CH2CH2CH2CH2CH2CH2CH3; 29.2 CH2CH2CH2CH2CH2CH2CH3; 28.4 and 29.0CH2CH2CH2CH2CH2CH2CH3; 22.6 CH2CH2CH2CH2CH2CH2CH3; 14.1 CH3. MS (ES+)m/z: 369 (100%) [M+H]+.4-[1-(2,4-Dichlorobenzyl)-5-heptyl-1H-1,2,4-triazol-3-yl]pyridine (
15a
) and 4-[1-(2,4-Dichlorobenzyl)-3-heptyl-1H-1,2,4-triazol-5-yl]pyridine (
15b
). Compounds15a and15b were prepared from5 (200 mg, 0.8 mmol), 2,4-dichlorobenzyl chloride (114 μL, 0.8 mmol), and (Bu)4NBr (12 mg, 0.04 mmol.); reaction time: 2 h. Yield: 118 mg of15a (36%) as a white solid and 7 mg of15b (2%) as a yellow oil.15a: Mp = 104-105°C. 1H-NMR (CDCl3) δ: 8.65 (d, 2H, J=6.1 Hz, Hm pyr); 7.93 (d, 2H, J=6.1 Hz, Ho pyr); 7.42 (d, 1H, J=2.2 Hz, Hm′ Ar); 7.19 (dd, 1H, J=8.4 Hz and 2.2 Hz, Hm Ar); 6.58 (d, 1H, J=8.4 Hz, Ho Ar); 5.39 (s, 2H, CH2Ar); 2.72 (t, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.70 (p, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.23 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.84 (bt, 3H, J=6.5 Hz, CH3). 13C-NMR (CDCl3) δ: 159.3 C3; 158.1 C5; 150.3 Cm pyr; 138.3 Cipso pyr; 134.8 Cipso Ar; 133.0 Cp Ar; 131.7 Co′ Ar; 129.5 Co and Cm′ Ar; 127.8 Cm Ar; 120.4 Co pyr; 48.9 (CH2Ar); 31.6 CH2CH2CH2CH2CH2CH2CH3; 29.1 CH2CH2CH2CH2CH2CH2CH3; 28.8 CH2CH2CH2CH2CH2CH2CH3; 27.7 CH2CH2CH2CH2CH2CH2CH3; 26.0CH2CH2CH2CH2CH2CH2CH3; 22.5 CH2CH2CH2CH2CH2CH2CH3; 14.0 CH3. MS (ES+)m/z: 403 (100%) [M+H]+. Anal (C21H24Cl2N4) % calculated (% found) C: 62.53 (62.28); H: 6.00 (6.30); N: 13.89 (14.04).15b: 1H-NMR (CDCl3) δ: 8.70 (d, 2H, J=6.1 Hz, Hm pyr); 7.43 (d, 1H, J=2.2 Hz, Hm′ Ar); 7.41 (d, 2H, J=6.1 Hz, Ho pyr); 7.19 (dd, 1H, J=8.1 Hz and 2.2 Hz, Hm Ar); 6.85 (d, 1H, J=8.1 Hz, Ho Ar); 5.44 (s, 2H, CH2Ar); 2.77 (t, 2H, J=7.6 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.73 (p, 2H, J=7.6 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.25 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.85 (ta, 3H, J=6.7 Hz, CH3). 13C-NMR (CDCl3) δ: 165.5 C3; 152.8 C5; 150.6 Cm pyr; 138.3 Cipso pyr; 135.1 Cipso Ar; 132.8 Cp Ar; 131.9 (Co′ Ar); 129.7 Co Ar; 128.8 Cm′ Ar; 127.8 Cm Ar; 122.3 Co pyr; 50.1 CH2Ar; 31.6 CH2CH2CH2CH2CH2CH2CH3; 29.3 CH2CH2CH2CH2CH2CH2CH3; 29.0 CH2CH2CH2CH2CH2CH2CH3; 28.3CH2CH2CH2CH2CH2CH2CH3; 22.6 CH2CH2CH2CH2CH2CH2CH3; 14.1 CH3. MS (ES+)m/z: 403 (100%) [M+H]+. HPLC: Acetonitrile/H2O 90 : 10, tR=30.3 min (90% purity).


### 2.1.5. General Procedure for the Synthesis of16–18

To a solution of the corresponding triazole (1 equiv) in dry CH2Cl2 (4–10 mL), excess of MeI was added. The reaction mixture was stirred at room temperature for the time indicated. Afterwards, solvent was removedin vacuo and the residue was purified by chromatography or recrystallization from Et2O/CH2Cl2.4-(1-Benzyl-5-heptyl-1H-1,2,4-triazol-3-yl)-1-methylpyridinium Iodide (
16
). Compound16 was prepared from13a (15 mg, 0.05 mmol) and MeI (4 μL, 0.07 mmol); reaction time: 16 h. Purification: flash chromatography [CH2Cl2/MeOH (99 : 1) → CH2Cl2/MeOH (9 : 1)]. Yield: 14 mg of16 (66%) as a yellow gummy solid. 1H-NMR (CDCl3) δ: 9.18 (d, 2H, J=6.7 Hz, Hm pyr); 8.54 (d, 2H, J=6.7 Hz, Ho pyr); 7.35 (m, 3H, Ph); 7.19 (m, 2H, Ph); 5.36 (s, 2H, CH2Ar); 4.68 (s, 3H, NMe); 2.71 (t, 2H, J=7.6 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.67 (p, 2H, J=7.6 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.23 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.85 (bt, 3H, J=7.0 Hz, CH3). 13C-NMR (CDCl3) δ: 159.0 C3; 155.3 C5; 146.7 Cipso pyr; 145.6 Cm pyr; 134.3 Cipso Ph; 129.1 Cm Ph; 128.6 Cp Ph; 127.3 Co Ph; 123.7 Co pyr; 53.0 CH2Ar; 49.1 NMe; 31.5 CH2CH2CH2CH2CH2CH2CH3; 29.0 CH2CH2CH2CH2CH2CH2CH3; 28.8 CH2CH2CH2CH2CH2CH2CH3; 27.2 CH2CH2CH2CH2CH2CH2CH3; 26.1CH2CH2CH2CH2CH2CH2CH3; 22.5 CH2CH2CH2CH2CH2CH2CH3; 14.0 CH3. MS (ES+)m/z: 349 (100%), [M]+. Anal (C22H29IN4) % calculated (% found) C: 55.47 (55.42); H: 6.14 (6.30); N: 11.76 (11.57).4-[1-(4-Chlorobenzyl)-5-heptyl-1H-1,2,4-triazol-3-yl]-1-methylpyridinium Iodide (
17
). Compound17 was prepared from14a (70 mg, 0.2 mmol) and MeI (140 μL 2.3 mmol); reaction time: 8 days. Purification: flash chromatography [CH2Cl2/MeOH (95 : 5)]. Yield: 87 mg of17 (90%) as a yellow gummy solid. 1H-NMR (CDCl3) δ: 9.21 (d, 2H, J=6.8 Hz, Hm pyr); 8.54 (d, 2H, J=6.8 Hz, Ho pyr); 7.33 (d, 2H, J=8.5 Hz, Hm Ar); 7.15 (d, 2H, J=8.5 Hz, Ho Ar); 5.33 (s, 2H, CH2Ar); 4.68 (s, 3H, NMe); 2.71 (t, 2H, J=7.5 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.69 (p, 2H, J=7.5 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.23 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.85 (bt, 3H, J=6.5 Hz, CH3). 13C-NMR (CDCl3) δ: 158.9 C3; 155.4 C5; 146.3 Cipso pyr; 145.7 Cm pyr; 134.4 Cipso Ar; 132.7 Cp Ar; 129.1 Cm Ar; 128.7 Co Ar; 123.5 Co pyr; 52.1 CH2Ar; 48.9 NMe; 31.4 CH2CH2CH2CH2CH2CH2CH3; 28.9 CH2CH2CH2CH2CH2CH2CH3; 28.6 CH2CH2CH2CH2CH2CH2CH3; 27.0 CH2CH2CH2CH2CH2CH2CH3; 25.9CH2CH2CH2CH2CH2CH2CH3; 22.4 CH2CH2CH2CH2CH2CH2CH3; 13.9 CH3. MS (ES+)m/z: 383 (100%) [M]+. Anal (C22H28ClIN4) % calculated (% found) C: 51.73 (51.58); H: 5.52 (5.41); N: 10.97 (10.72).4-[1-(2,4-Dichlorobenzyl)-5-heptyl-1H-1,2,4-triazol-3-yl]-1-methylpyridinium Iodide (
18
). Compound18 was prepared from15a (50 mg, 0.1 mmol) and MeI (277 μL 4.45 mmol); reaction time: 8 days. Purification: recrystallization from Et2O/CH2Cl2. Yield: 43 mg of18 (64%) as a yellow solid. Mp = 136–138°C. 1H-NMR (CDCl3) δ: 9.22 (d, 2H, J=6.7 Hz, Hm pyr); 8.53 (d, 2H, J=6.7 Hz, Ho pyr); 7.44 (d, 1H, J=1.9 Hz, Hm′ Ar); 7.25 (dd, 1H, J=8.4 Hz and 1.9 Hz, Hm Ar); 7.00 (d, 1H, J=8.4 Hz, Ho Ar); 5.42 (s, 2H, CH2Ar); 4.69 (s, 3H, NMe); 2.76 (t, 2H, J=7.6 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.72 (p, 2H, J=7.6 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.26 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.86 (bt, 3H, J=7.3 Hz, CH3). 13C-NMR (CDCl3) δ: 159.4 C3; 155.8 C5; 146.5 Cipso pyr; 145.7 Cm pyr; 135.4 Cipso Ar; 133.4 Cp Ar; 130.6 Co′ Ar; 130.3 Co Ar; 129.7 Cm′ Ar; 128,0 (Cm BnAr); 123,7 (Co CAr); 49,5 (CH2Ar); 49,2 (NMe); 31.5 CH2CH2CH2CH2CH2CH2CH3; 29.0 CH2CH2CH2CH2CH2CH2CH3; 28.8 CH2CH2CH2CH2CH2CH2CH3; 27.3 CH2CH2CH2CH2CH2CH2CH3; 25.9CH2CH2CH2CH2CH2CH2CH3; 22.5 CH2CH2CH2CH2CH2CH2CH3; 14.0 CH3. MS (ES+)m/z: 417 (100%) [M]+. Anal (C22H27Cl2IN4) % calculated (% found) C: 48.46 (48.71); H: 4.99 (5.20); N: 10.27 (10.06).


## 2.1.1. General

All reagents and solvents were used as commercially received. EtOH was dried over magnesium. TLC was carried out by precoated silica-gel 60 F254 plates (Merck) and detection by UV light (254 nm). Flash-column chromatography was carried out by Kieselgel 60 (230–400 mesh; Merck). Medium pressure chromatography (MPLC) was carried out by Flash Master Personal system with prepacked silica-gel cartridges. The purity of the final compounds was determined by elemental analysis or analytical HPLC. Elemental analysis was performed on a Heraeus CHN-O rapid analyzer. Analyses indicated by the symbols of the elements or functions were within ±0.4% of the theoretical values, except compound6. Analytical HPLC was run on a Waters 6000 with Delta Pak C 18.5 mm, 300 Ǻ (3.9 × 150 mm) column, using an eluent Acetonitrile/H2O (0.05% H3PO4 + 0.04% TEA) in the proportion indicated in each case; flow rate used was 1 mL/min and the UV absorption was detected at a wavelength of 254 nm. HPLC analyses were within ≥90% of purity, except compound11b (81% purity). The mass spectra (electrospray positive mode) were determined on a MSD-Series 1100 Hewlett Packard instrument. Melting points (uncorrected) were determined with a Reichert Jung Thermovar apparatus. 1H and 13C NMR spectra were recorded on a Gemini 200, Varian 300 and 400 unity spectrometers using TMS as the internal standard. All chemical shifts are reported in ppm. For the assignment of the protons and carbons of the aromatic rings Scheme 1 is used.Scheme 1


## 2.1.2. General Procedure for the Synthesis of1 and2

To a suspension of the corresponding nitrile (10 equiv) in dry EtOH (30–75 mL) NaOMe (1 equiv) was added. It was stirred at room temperature under N2 atmosphere for 48 h. Afterwards, ammonium chloride (10 equiv) was added, and the stirring was maintained for 24 more hours. Then, unreacted ammonium chloride was filtered off and the solvent was evaporated from the liquid layer. The white solid obtained was washed with Et2O, dried, and used in the next step without further purification.4-Chlorobenzimidamide Hydrochloride (
1
). Compound1 was prepared from 4-chlorobenzonitrile (10.00 g, 72.7 mmol), NaOMe (393 mg, 7.3 mmol), and ammonium chloride (3.90 g, 72.7 mmol). Yield: 4.34 g of1 (31%) as a white solid. Mp = 246-247°C (236–240°C (EtOH)). [13] 1H-NMR (CD3OD) δ: 8.02 (d, 2H, J=9.0 Hz, Ho); 7.84 (d, 2H, J=9.0 Hz, Hm). 13C-NMR (CD3OD) δ: 167.6 C=NH; 141.4 Cp; 130.8 and 130.7 Co and Cm; 128.2 Cipso. MS (ES+)m/z: 155 (100%) [M+H]+.4-Amidinopyridinium Hydrochloride (
2
). Compound2 was prepared from 4-cyanopyridine (2.50 g, 24.0 mmol), NaOMe (130 mg, 2.4 mmol), and ammonium chloride (1.28 g, 24.0 mmol). Yield: 3.30 g of2 (87%) as a white solid. Mp = 248-249°C. 1H-NMR (CD3OD) δ: 8.93 (d, 2H, J=6.2 Hz, Hm); 7.87 (d, 2H, J=6.2 Hz, Ho). 13C-NMR (CD3OD) δ: 166.9 C=NH; 151.7 Cm; 138.1 Cipso; 123.2 Co. MS (ES+)m/z: 122 (100%) [M+H]+.


## 2.1.3. General Procedure for the Synthesis of3–5

To a solution of the corresponding amidinium salt (1.5 equiv) in dry EtOH (10–45 mL), NaOMe (1 equiv) in 10 mL of dry EtOH was added. The suspension was stirred at room temperature for 1 h. Then, the solid formed was filtered on Celite. Octanoic hydrazide (2 equiv) was added to the liquid layer and the mixture was stirred under reflux for 46–49 h. After cooling the reaction mixture, solvent was removedin vacuo. The residue was dissolved in CH2Cl2 and washed with water (3 × 20 mL). The organic layer was dried over anhydrous Na2SO4 and the solvent was removedin vacuo. The obtained residue was purified by MPLC using cyclohexane/EtOAc (3 : 1) as eluent, except for compound5 where cyclohexane/EtOAc (3 : 1 to 4 : 1) was used.3-Heptyl-5-phenyl-1H-1,2,4-triazole (
3
). Compound3 was prepared from benzamidine hydrochloride hydrate (857 mg, 5.5 mmol), octanoic hydrazide (581 mg, 3.6 mmol), and NaOMe (394 mg, 7.3 mmol). Yield: 683 mg of3 (78%) as a transparent oil. Mp = 129–132°C oxalate (to a solution of the free base in Et2O, a solution of oxalic acid in EtOAc was added; the white solid was filtered off, washed with EtOAc, and dried). 1H-NMR (CDCl3) δ: 10.65 (bs, 1H, NH); 7.96 (m, 2H, Ho); 7.34 (m, 3H, Hm and Hp); 2.69 (t, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.65 (p, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.16 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.79 (bt, 3H, J=6.3 Hz, CH3). 13C-NMR (CDCl3) δ: 160.4 and 160.0 C3 and C5; 129.8 Cipso; 129.5 Cp; 128.6 Cm; 126.4 Co; 31.5 CH2CH2CH2CH2CH2CH2CH3; 29.1 CH2CH2CH2CH2CH2CH2CH3; 28.8 CH2CH2CH2CH2CH2CH2CH3; 28.1 CH2CH2CH2CH2CH2CH2CH3; 27.0CH2CH2CH2CH2CH2CH2CH3; 22.5 CH2CH2CH2CH2CH2CH2CH3; 13.9 CH3. MS (ES+)m/z: 244 (100%) [M+H]+. Anal (C15H21N3·C2H2O4) % calculated (% found) C: 61.25 (61.41); H: 6.95 (7.12); N: 12.60 (12.62).5-(4-Chlorophenyl)-3-heptyl-1H-1,2,4-triazole (
4
). Compound4 was prepared from1 (1.00 g, 5.2 mmol), octanoic hydrazide (549 mg, 3.5 mmol), and NaOMe (375 mg, 7.0 mmol). Yield 392 mg of4 (40%) as a white solid. Mp = 108–111°C. 1H-NMR (CDCl3) δ: 7.91 (d, 2H, J=8.6 Hz, Ho); 7.34 (d, 2H, J=8.6 Hz, Hm); 2.72 (t, 2H, J=7.6 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.68 (p, 2H, J=7.6 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.20 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.82 (bt, 3H, J=6.5 Hz, CH3). 13C-NMR (CDCl3) δ: 160.4 and 159.5 C3 and C5; 135.5 Cipso; 128.9 Cm; 128.7 Cp; 127.7 Co; 31.6 CH2CH2CH2CH2CH2CH2CH3; 29.2 CH2CH2CH2CH2CH2CH2CH3; 28.8 CH2CH2CH2CH2CH2CH2CH3; 28.0 CH2CH2CH2CH2CH2CH2CH3; 26.9CH2CH2CH2CH2CH2CH2CH3; 22.5 CH2CH2CH2CH2CH2CH2CH3; 14.0 CH3. MS (ES+)m/z: 278 (100%) [M+H]+. Anal (C15H20ClN3) % calculated (% found) C: 64.85 (65.09); H: 7.26 (7.42); N: 15.13 (15.35).4-(3-Heptyl-1H-1,2,4-triazol-5-yl)pyridine (
5
) and N′-[imino(pyridin-4-yl)methyl]octa-nehydrazide (6). Compound5 was prepared from2 (2.00 g, 12.7 mmol), octanoic hydrazide (1.35 g, 8.5 mmol), and NaOMe (918 mg, 17.0 mmol). Yield: 459 mg of5 (23%) as a white solid and 1.61 g of6 (45%) as a white solid.5: Mp = 109–112°C. 1H-NMR (CDCl3) δ: 8.69 (d, 2H, J=6.1 Hz, Hm); 8.06 (d, 2H, J=6.1 Hz, Ho); 2.85 (t, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.78 (p, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.21 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.81 (bt, 3H, J=6.7 Hz, CH3). 13C-NMR (CDCl3) δ: 159.4 and 159.2 C3 and C5; 149.4 Cm; 139.5 Cipso; 121.0 Co; 31.5 CH2CH2CH2CH2CH2CH2CH3; 29.1 CH2CH2CH2CH2CH2CH2CH3; 28.8 CH2CH2CH2CH2CH2CH2CH3; 28.1 CH2CH2CH2CH2CH2CH2CH3; 26.7CH2CH2CH2CH2CH2CH2CH3; 22.5 CH2CH2CH2CH2CH2CH2CH3; 13.9 CH3. MS (ES+)m/z: 245 (100%) [M+H]+. Anal (C14H20N4) % calculated (% found) C: 68.82 (68.71); H: 8.25 (8.36); N: 22.93 (22.78).6: Mp = 135–138°C. 1H-NMR (CD3OD) δ: 8.67 (d, 2H, J=6.1 Hz, Hm); 7.94 (d, 2H, J=6.1 Hz, Ho); 2.41 (t, 2H, J=7.4 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.76 (m, 2H, CH2CH2CH2CH2CH2CH2CH3); 1.41 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0,99 (bt, 3H, J=6.0 Hz, CH3). 13C-NMR (CD3OD) δ: 172.7 CONH; 151.7 C=NH; 150.4 Cm; 144.1 Cipso; 122.9 Co; 35.7CH2CH2CH2CH2CH2CH2CH3; 32.9 CH2CH2CH2CH2CH2CH2CH3; 30.4 and 30.2 CH2CH2CH2CH2CH2CH2CH3; 27.1 CH2CH2CH2CH2CH2CH2CH3; 23.7 CH2CH2CH2CH2CH2CH2CH3; 14.4 CH3. MS (ES+)m/z: 263 (100%) [M+H]+. Anal (C14H22N4O·1/2HCl) % calculated (% found) C: 59.93 (59.10); H: 8.08 (8.11); N: 19.97 (20.45).Intermediate6 (1.00 g, 3.6 mmol) in dry EtOH (20 mL) reacted by refluxing with NaOMe (1.03 g, 19.0 mmol) for 4 days. Under this procedure,5 was obtained in 78% yield (676 mg).


## 2.1.4. General Procedure for the Synthesis of7a–15a and7b–15b

To a solution of the 3,5-disubstituted triazole (1 equiv) in 40% NaOH aq solution (3–5 mL) and toluene (7–10 mL) (Bu)4NBr (0.05 equiv) were first added, and later the corresponding alkylating agent (1 equiv) was added. The reaction mixture was stirred at 80–90°C (bath temperature) for the reaction time indicated. Afterwards, organic layer was separated and the aqueous layer was extracted with CH2Cl2 (3 × 25 mL). The combined organic layers were dried over anhydrous Na2SO4 and the solvent was removedin vacuo. The residue was purified by MPLC [cyclohexane/EtOAc (9 : 1)], except for compound13a, which was purified by flash chromatography [CH2Cl2 → CH2Cl2/MeOH (60 : 1)].1-Benzyl-5-heptyl-3-phenyl-1H-1,2,4-triazole (
7a
) and 1-Benzyl-3-heptyl-5-phenyl-1H-1,2,4-triazole (
7b
). Compounds7a and7b were prepared from3 (100 mg, 0.4 mmol), benzyl bromide (52 μL, 0.4 mmol), and (Bu)4NBr (6 mg, 0.02 mmol); reaction time: 1.5 h. Yield: 106 mg of7a (78%) as a transparent oil, and 13 mg of7b (9%) as a transparent oil.7a: 1H-NMR (CDCl3) δ: 8.08 (m, 2H, Ph); 7.40 (m, 3H, Ph); 7.31 (m, 3H, Bn); 7.18 (m, 2H, Bn); 5.34 (s, 2H, CH2Ph); 2.69 (t, 2H, J=7.8 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.64 (p, 2H, J=7.5 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.22 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.85 (bt, 3H, J=6.1 Hz, CH3). 13C-NMR (CDCl3) δ: 160.8 C3; 157.0 C5; 135.7 Cipso Bn; 131.2 Cipso Ph; 128.9 Cm Bn and Cp Ph; 128.5 Cm Ph; 126.9 Co Bn; 126.3 Co Ph; 128.1 Cp Bn; 52.1 (CH2Ph); 31.6 CH2CH2CH2CH2CH2CH2CH3; 29.2 CH2CH2CH2CH2CH2CH2CH3; 28.8 CH2CH2CH2CH2CH2CH2CH3; 27.8 CH2CH2CH2CH2CH2CH2CH3; 26.2CH2CH2CH2CH2CH2CH2CH3; 22.5 CH2CH2CH2CH2CH2CH2CH3; 14.0 CH3. MS (ES+)m/z: 334 (100%) [M+H]+. Anal (C22H27N3) % calculated (% found) C: 79.24 (79.35); H: 8.16 (8.40); N: 12.60 (12.64).7b: 1H-NMR (CDCl3) δ: 7.53 (m, 2H, Ph); 7.41 (m, 3H, Ph); 7.30 (m, 3H, Bn); 7.14 (m, 2H, Bn); 5.35 (s, 2H, CH2Ph); 2.75 (t, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.78 (p, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.23 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.85 (bt, 3H, J=6.4 Hz, CH3). 13C-NMR (CDCl3) δ: 164.1 C3; 155.3 C5; 136.1 Cipso Bn; 130.2 Cipso Ph; 128.9 Cm Bn; 128.8 and 128.7 Co and Cm Ph; 126.7 Co Bn; 127.9 Cp Bn and Cp Ph; 52.4 CH2Ph; 31.8 CH2CH2CH2CH2CH2CH2CH3; 29.3 CH2CH2CH2CH2CH2CH2CH3; 29.0 CH2CH2CH2CH2CH2CH2CH3; 28.5 CH2CH2CH2CH2CH2CH2CH3; 28.3CH2CH2CH2CH2CH2CH2CH3; 22.6 CH2CH2CH2CH2CH2CH2CH3; 14.1 CH3. MS (ES+)m/z: 334 (100%) [M+H]+. Anal (C22H27N3) % calculated (% found) C: 79.24 (79.10); H: 8.16 (7.95); N: 12.60 (12.42).1-(4-Chlorobenzyl)-5-heptyl-3-phenyl-1H-1,2,4-triazole (
8a
) and 1-(4-Chlorobenzyl)-3-heptyl-5-phenyl-1H-1,2,4-triazole (
8b
). Compounds8a and8b were prepared from3 (73 mg, 0.3 mmol), 4-chlorobenzyl chloride (48 mg, 0.3 mmol), and (Bu)4NBr (6 mg, 0.02 mmol); reaction time: 11 h. Yield: 96 mg of8a (87%) as a yellow solid and 10 mg of8b (9%) as a transparent oil.8a: 1H-NMR (CDCl3) δ: 8.07 (m, 2H, Ho Ph); 7.37 (m, 3H, Hm and Hp Ph); 7.29 (d, 2H, J=8.4 Hz, Hm Bn); 7.11 (d, 2H, J=8.4 Hz, Ho Bn); 5.27 (s, 2H, CH2Ar); 2.67 (t, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.69 (p, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.24 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.85 (ta, 3H, J=6.9 Hz, CH3). 13C-NMR (CDCl3) δ: 161.0 C3; 156.9 C5; 134.2 Cipso Bn; 134.0 Cp Bn; 131.1 Cipso Ph; 129.0 and 128.3 Co Bn, Cm Bn and Cp Ph; 128.5 Cm Ph; 126.2 Co Ph; 51.3 CH2Ar; 31.5 CH2CH2CH2CH2CH2CH2CH3; 29.2 CH2CH2CH2CH2CH2CH2CH3; 28.8 CH2CH2CH2CH2CH2CH2CH3; 27.7 CH2CH2CH2CH2CH2CH2CH3; 26.1CH2CH2CH2CH2CH2CH2CH3; 22.5 CH2CH2CH2CH2CH2CH2CH3; 14.0 CH3. MS (ES+)m/z: 368 (100%) [M+H]+. Anal (C22H26ClN3) % calculated (% found) C: 71.82 (71.75); H: 7.12 (6.98); N: 11.42 (11.63).8b: 1H-NMR (CDCl3) δ: 7.50 (m, 2H, H Ph); 7.43 (m, 3H, Ph); 7.29 (d, 2H, J=8.4 Hz, Hm Bn); 7.06 (d, 2H, J=8.4 Hz, Ho Bn); 5.30 (s, 2H, CH2Ar); 2.75 (t, 2H, J=7.5 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.77 (p, 2H, J=7.5 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.23 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.85 (ta, 3H, J=6.2 Hz, CH3). 13C-NMR (CDCl3) δ: 164.6 C3; 155.5 C5; 134.7 Cipso Bn; 133.8 Cp Bn; 130.1 Cp Ph; 129.0 Cm Bn; 128.8 Co Bn; 128.6 Cm Ph; 128.1 Co Ph; 128.0 Cipso Ph; 51.7 CH2Ar; 31.8 CH2CH2CH2CH2CH2CH2CH3; 29.4 CH2CH2CH2CH2CH2CH2CH3; 29.0 CH2CH2CH2CH2CH2CH2CH3; 28.5CH2CH2CH2CH2CH2CH2CH3; 22.6 CH2CH2CH2CH2CH2CH2CH3; 14.1 CH3. MS (ES+)m/z: 368 (100%) [M+H]+. HPLC: Acetonitrile/H2O 95 : 5, tR=28.5 min (99% purity).1-(2,4-Dichlorobenzyl)-5-heptyl-3-phenyl-1H-1,2,4-triazole (
9a
) and 1-(2,4-Dichloro-benzyl)-3-heptyl-5-phenyl-1H-1,2,4-triazole (
9b
). Compounds9a and9b were prepared from3 (100 mg, 0.4 mmol), 2,4-dichlorobenzyl chloride (57 μL, 0.4 mmol), and (Bu)4NBr (6 mg, 0.02 mmol); reaction time: 5 h. Yield: 123 mg of9a (74%) as a white solid and 12 mg of9b (7%) as a transparent oil.9a: Mp = 70–73°C. 1H-NMR (CDCl3) δ: 8.11 (m, 2H, Ho Ph); 7.42 (m, 4H, Hm′ Bn, Hm and Hp Ph); 7.19 (dd, 1H,J = 8.3 Hz and 1.6 Hz, Hm Ph); 6.85 (d, 1H, J=8.3 Hz, Ho Bn); 5.41 (s, 2H, CH2Ar); 2.73 (t, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.73 (p, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.27 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.88 (bt, 3H, J=6.4 Hz, CH3). 13C-NMR (CDCl3) δ: 161.4 C3; 157.4 C5; 134.5 Cipso Bn; 132.8 Cp Bn; 132.2 Co′ Bn; 131.0 Cipso Ph; 129.3 Co Bn; 129.2 Cm′ Bn; 129.1 Cp Ph; 128.5 Cm Ph; 127.7 Cm Bn; 126.3 Co Ph; 48.6 CH2Ar; 31.5 CH2CH2CH2CH2CH2CH2CH3; 29.1 CH2CH2CH2CH2CH2CH2CH3; 28.8 CH2CH2CH2CH2CH2CH2CH3; 27.8 CH2CH2CH2CH2CH2CH2CH3; 26.0CH2CH2CH2CH2CH2CH2CH3; 22.5 CH2CH2CH2CH2CH2CH2CH3; 14.0 CH3. MS (ES+)m/z: 402 (100%) [M+H]+. Anal (C22H25Cl2N3) % calculated (% found) C: 65.67 (65.42); H: 6.26 (6.50); N: 10.44 (10.35).9b: 1H-NMR (CDCl3) δ: 7.50–7.43 (m, 5H, Ph); 7.40 (d, 1H, J=1.7 Hz, Hm′ Bn); 7.20 (dd, 1H, J=8.6 Hz and 1.7 Hz, Hm Bn); 6.84 (d, 1H, J=8.6 Hz, Ho Bn); 5.39 (s, 2H, CH2Ar); 2.76 (t, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.79 (p, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.23 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.85 (bt, 3H, J=6.1 Hz, CH3). 13C-NMR (CDCl3) δ: 165.2 C3; 156.2 C5; 134.6 Cipso Bn; 133.0 Cp Bn; 132.9 Co′ Bn; 130.5 Co Bn; 129.7 Cm′ Bn; 129.2 Cm Ph; 129.0 Cp Ph; 128.6 Co Ph; 127.9 Cm Bn; 127.8 Cipso Ph; 50.1 CH2Ar; 32.0 CH2CH2CH2CH2CH2CH2CH3; 29.6 CH2CH2CH2CH2CH2CH2CH3; 29.2 CH2CH2CH2CH2CH2CH2CH3; 28.7CH2CH2CH2CH2CH2CH2CH3; 22.9 CH2CH2CH2CH2CH2CH2CH3; 14.3 CH3. MS (ES+)m/z: 402 (100%) [M+H]+. Anal (C22H25Cl2N3·C6H12) % calculated (% found) C: 69.12 (69.42); H: 7.67 (7.79); N: 8.64 (8.24).1-Benzyl-3-(4-chlorophenyl)-5-heptyl-1H-1,2,4-triazole (
10a
) and 1-Benzyl-5-(4-chlo-rophenyl)-3-heptyl-1H-1,2,4-triazole (
10b
). Compounds10a and10b were prepared from4 (80 mg, 0.3 mmol), benzyl bromide (34 μL, 0.3 mmol), and (Bu)4NBr (6 mg, 0.02 mmol); reaction time: 20 min. Yield: 94 mg of10a (89%) as a white solid and 9 mg of10b (8%) as a yellow oil.10a: Mp = 47–50°C. 1H-NMR (CDCl3) δ: 8.02 (d, 2H, J=8.8 Hz, Ho Ar); 7.36 (d, 2H, J=8.8 Hz, Hm Ar); 7.29 (m, 3H, Bn); 7.19 (m, 2H, Bn); 5.31 (s, 2H, CH2Ph); 2.67 (t, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.66 (p, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.22 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.85 (bt, 3H, J=6.9 Hz, CH3). 13C-NMR (CDCl3) δ: 159.9 C3; 157.1 C5; 135.5 Cipso Ar; 134.7 Cipso Bn; 129.8 Cp Ar; 128.9 Cm Ar; 128.6 Cm Bn; 128.1 Cp Bn; 127.6 Co Ar; 126.9 Co Bn; 52.1 (CH2Ph); 31.5 CH2CH2CH2CH2CH2CH2CH3; 29.2 CH2CH2CH2CH2CH2CH2CH3, 28.8 CH2CH2CH2CH2CH2CH2CH3; 27.7 CH2CH2CH2CH2CH2CH2CH3; 26.1CH2CH2CH2CH2CH2CH2CH3; 22.5 CH2CH2CH2CH2CH2CH2CH3; 14.0 CH3. MS (ES+)m/z: 368 (100%) [M+H]+. Anal (C22H26ClN3) % calculated (% found) C: 71.82 (72.02); H: 7.12 (6.89); N: 11.42 (11.24).10b: 1H-NMR (CDCl3) δ: 7.47 (d, 2H, J=8.5 Hz, Hm Ar); 7.38 (d, 2H, J=8.5 Hz, Ho Ar); 7.30 (m, 3H, Bn); 7.00 (m, 2H, Bn); 5.33 (s, 2H, CH2Ph); 2.75 (t, 2H, J=7.6 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.78 (p, 2H, J=7.6 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.25 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.85 (bt, 3H, J=6.4 Hz, CH3). 13C-NMR (CDCl3) δ: 164.6 C3; 155.0 C5; 136.3 Cipso Ar; 136.2 Cipso Bn; 130.0 Cm Ar; 129.4 Cp Ar; 129.1 Co Ar; 129.0 Cm Bn; 128.0 Cp Bn; 52.6 (CH2Ph); 31.8 CH2CH2CH2CH2CH2CH2CH3; 29.4 CH2CH2CH2CH2CH2CH2CH3; 29.0 CH2CH2CH2CH2CH2CH2CH3; 28.5CH2CH2CH2CH2CH2CH2CH3; 22.6 CH2CH2CH2CH2CH2CH2CH3; 14.0 CH3. MS (ES+)m/z: 368 (100%) [M+H]+.1-(4-Chlorobenzyl)-3-(4-chlorophenyl)-5-heptyl-1H-1,2,4-triazole (
11a
) and 1-(4-Chlo-robenzyl)-5-(4-chlorophenyl)-3-heptyl-1H-1,2,4-triazole (
11b
). Compounds11a and11b were prepared from4 (100 mg, 0.4 mmol), 4-chlorobenzyl chloride (64 mg, 0.4 mmol), and (Bu)4NBr (6 mg, 0.02 mmol); reaction time: 6 h. Yield: 132 mg of11a (91%) as a white solid and 2 mg of11b (1%) as a transparent oil.11a: Mp = 58–60°C. 1H-NMR (CDCl3) δ: 8.00 (d, 2H, J=8.8 Hz, Ho Ar); 7.35 (d, 2H, J=8.8 Hz, Hm Ar); 7.28 (d, 2H, J=8.4 Hz, Hm CH2Ar); 7.10 (d, 2H, J=8.4 Hz, Ho CH2Ar); 5.26 (s, 2H, CH2Ar); 2.66 (t, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.66 (p, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.23 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.84 (bt, 3H, J=6.3 Hz, CH3). 13C-NMR (CDCl3) δ: 160.1 C3; 157.1 C5; 134.8 Cipso Ar; 134.1 Cipso CH2Ar; 134.0 Cp CH2Ar; 129.6 Cp Ar; 129.0 Cm Ar; 128.6 Cm CH2Ar; 128.3 Co CH2Ar; 127.5 Co Ar; 51.3 (CH2Ar); 31.5 CH2CH2CH2CH2CH2CH2CH3; 29.2 CH2CH2CH2CH2CH2CH2CH3; 28.8 CH2CH2CH2CH2CH2CH2CH3; 27.7 CH2CH2CH2CH2CH2CH2CH3; 26.1CH2CH2CH2CH2CH2CH2CH3; 22.5 CH2CH2CH2CH2CH2CH2CH3; 14.0 CH3. MS (ES+)m/z: 402 (100%) [M+H]+. Anal (C22H25Cl2N3) % calculated (% found) C: 65.67 (65.72); H: 6.26 (6.12); N: 10.44 (10.40).11b: 1H-NMR (CDCl3) δ: 7.45 (d, 2H, J=9.0 Hz, Ho Ar); 7.39 (d, 2H, J=9.0 Hz, Hm Ar); 7.30 (d, 2H, J=8.3 Hz, Hm CH2Ar); 7.05 (d, 2H, J=8.3 Hz, Ho CH2Ar); 5.29 (s, 2H, CH2Ar); 2.70 (t, 2H, J=7.8 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.75 (p, 2H, J=7.8 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.23 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.82 (bt, 3H, J=6.2 Hz, CH3). 13C-NMR (CDCl3) δ: 164.7 C3; 154.4 C5; 136.4 Cipso Ar; 134.4 Cipso CH2Ar; 134.0 Cp CH2Ar; 129.9 Cm and Cp Ar; 129.2 Co Ar and Cm CH2Ar; 128.0 Co CH2Ar; 51.8 CH2Ar; 31.8 CH2CH2CH2CH2CH2CH2CH3; 29.7 CH2CH2CH2CH2CH2CH2CH3; 29.3 CH2CH2CH2CH2CH2CH2CH3; 29.0 CH2CH2CH2CH2CH2CH2CH3; 28.4CH2CH2CH2CH2CH2CH2CH3; 22.6 CH2CH2CH2CH2CH2CH2CH3; 14.1 CH3. MS (ES+)m/z: 402 (100%) [M+H]+. HPLC: Acetonitrile/H2O 95 : 5, tR=25.5 min (81% purity).3-(4-Chlorophenyl)-1-(2,4-dichlorobenzyl)-5-heptyl-1H-1,2,4-triazole (
12a
) and 5-(4-Chlorophenyl)-1-(2,4-dichlorobenzyl)-3-heptyl-1H-1,2,4-triazole (
12b
). Compounds12a and12b were prepared from4 (90 mg, 0.3 mmol), 2,4-dichlorobenzyl chloride (45 μL, 0.3 mmol), and (Bu)4NBr (6 mg, 0.02 mmol); reaction time: 6 h. Yield: 122 mg of12a (86%) as a white solid and 8 mg of12b (6%) as a transparent oil.12a: Mp = 97–99°C. 1H-NMR (CDCl3) δ: 8.00 (d, 2H, J=8.6 Hz, Ho Ar); 7.41 (d, 1H, J=2.0 Hz, Hm′ CH2Ar); 7.37 (d, 2H, J=8.6 Hz, Hm Ar); 7.18 (dd, 1H, J=8.4 Hz and 2.0 Hz, Hm CH2Ar); 6.84 (d, 1H, J=8.4 Hz, Ho CH2Ar); 5.37 (s, 2H, CH2Ar); 2.70 (t, 2H, J=7.6 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.69 (p, 2H, J=7.6 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.22 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.85 (bt, 3H, J=7.1 Hz, CH3). 13C-NMR (CDCl3) δ: 160.5 C3; 157.6 C5; 135.0 Cipso Ar; 134.6 Cipso CH2Ar; 132.9 Cp CH2Ar; 132.0 Co′ CH2Ar; 129.5 Cp Ar; 129.4 Co CH2Ar; 129.3 Cm′ CH2Ar; 128.7 Cm Ar; 127.7 Cm CH2Ar; 127.6 Co Ar; 48.7 CH2Ar; 31.5 CH2CH2CH2CH2CH2CH2CH3; 29.1 CH2CH2CH2CH2CH2CH2CH3; 28.8 CH2CH2CH2CH2CH2CH2CH3; 27.7 CH2CH2CH2CH2CH2CH2CH3; 26.0CH2CH2CH2CH2CH2CH2CH3; 22.5 CH2CH2CH2CH2CH2CH2CH3; 14.0 CH3. MS (ES+)m/z: 436 (100%) [M+H]+. Anal (C22H24Cl3N3) % calculated (% found) C: 60.49 (60.42); H: 5.54 (5.74); N: 9.62 (9.42).12b: 1H-NMR (CDCl3) δ: 7.41 (bs, 5H, Ho Ar, Hm Ar and Hm′ CH2Ar); 7.21 (dd, 1H, J=8.3 Hz and 2.0 Hz, Hm CH2Ar); 6.84 (d, 1H, J=8.3 Hz, Ho CH2Ar); 5.37 (s, 2H, CH2Ar); 2.75 (t, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.77 (p, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1,23 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.85 (bt, 3H, J=6.1 Hz, CH3). 13C-NMR (CDCl3) δ: 165.1 C3; 154.9 C5; 136.6 Cipso Ar; 134.7 Cipso CH2Ar; 132.8 Cp CH2Ar; 132.5 Co′ CH2Ar; 129.7 Cm and Cp Ar; 129.6 Co CH2Ar; 129.3 Co Ar; 129.0 Cm′ CH2Ar; 127.8 Cm CH2Ar; 49.9 CH2Ar; 31.8 CH2CH2CH2CH2CH2CH2CH3; 29.7 CH2CH2CH2CH2CH2CH2CH3; 29.3 CH2CH2CH2CH2CH2CH2CH3; 29.0 CH2CH2CH2CH2CH2CH2CH3; 28.4CH2CH2CH2CH2CH2CH2CH3; 22.6 CH2CH2CH2CH2CH2CH2CH3; 14.0 CH3. MS (ES+)m/z: 436 (99%) [M+H]+. HPLC: Acetonitrile/H2O 90 : 10, tR=66.2 min (99% purity).4-(1-Benzyl-5-heptyl-1H-1,2,4-triazol-3-yl)pyridine (
13a
). Compound13a was prepared from5 (150 mg, 0.6 mmol), benzyl chloride (73 μL, 0.6 mmol), and (Bu)4NBr (6 mg, 0.02 mmol); reaction time: 2.5 h. Yield: 166 mg of13a (81%) as an orange oil. 1H-NMR (CDCl3) δ: 8.63 (d, 2H, J=6.0 Hz, Hm pyr); 7.93 (d, 2H, J=6.0 Hz, Ho pyr); 7.31 (m, 3H, Ph); 7.16 (m, 2H, Ph); 5.33 (s, 2H, CH2Ar); 2.68 (t, 2H, J=7.8 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.65 (p, 2H, J=7.0 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.21 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.83 (bt, 3H, J=7.1 Hz, CH3). 13C-NMR (CDCl3) δ: 158.7 C3; 157.5 C5; 150.1 Cm pyr; 138.5 Cipso pyr; 135.2 Cipso Ph; 128.9 Cm Ph; 128.2 Cp Ph; 127.0 Co Ph; 120.4 Co pyr; 52.3 CH2Ph; 31.5 CH2CH2CH2CH2CH2CH2CH3; 29.1 CH2CH2CH2CH2CH2CH2CH3; 28.8 CH2CH2CH2CH2CH2CH2CH3; 27.6 CH2CH2CH2CH2CH2CH2CH3; 26.1CH2CH2CH2CH2CH2CH2CH3; 22.5 CH2CH2CH2CH2CH2CH2CH3; 14.0 CH3. MS (ES+)m/z: 335 (100%) [M+H]+. Anal (C21H26N4) % calculated (% found) C: 75.41 (75.71); H: 7.84 (7.79); N: 16.75 (16.54).4-[1-(4-Chlorobenzyl)-5-heptyl-1H-1,2,4-triazol-3-yl]pyridine (
14a
) and 4-[1-(4-Chlo-robenzyl)-3-heptyl-1H-1,2,4-triazol-5-yl]pyridine (
14b
). Compounds14a and14b were prepared from5 (180 mg, 0.7 mmol), 4-chlorobenzyl chloride (119 mg, 0.7 mmol), and (Bu)4NBr (12 mg, 0.04 mmol); reaction time: 6 h. Yield: 150 mg of14a (51%) as a brown solid and 10 mg of14b (4%) as a brown oil.14a: Mp = 77–80°C. 1H-NMR (CDCl3) δ: 8.61 (d, 2H, J=6.0 Hz, Hm pyr); 7.90 (d, 2H, J=6.0 Hz, Ho pyr); 7.27 (d, 2H, J=8.6 Hz, Hm Ar); 7.09 (d, 2H, J=8.6 Hz, Ho Ar); 5.27 (s, 2H, CH2Ar); 2.66 (t, 2H, J=7.8 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.65 (p, 2H, J=7.8 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.20 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.82 (bt, 3H, J=6.6 Hz, CH3). 13C-NMR (CDCl3) δ: 158.9 C3; 157.5 C5; 150.2 Cm pyr; 138.4 Cipso pyr; 134.2 Cipso Ar; 133.7 Cp Ar; 129.1 Cm Ar; 128.4 Co Ar; 120.4 Co pyr; 51.8 CH2Ar; 31.5 CH2CH2CH2CH2CH2CH2CH3; 29.1 CH2CH2CH2CH2CH2CH2CH3; 28.8 CH2CH2CH2CH2CH2CH2CH3; 26.0CH2CH2CH2CH2CH2CH2CH3; 22.5 CH2CH2CH2CH2CH2CH2CH3; 14.0 CH3. MS (ES+)m/z: 369 (100%) [M+H]+. Anal (C21H25ClN4) % calculated (% found) C: 68.37 (68.55); H: 6.83 (6.90); N: 15.19 (15.21).14b: 1H-NMR (CDCl3) δ: 8.65 (d, 2H, J=6.2 Hz, Hm pyr); 7.44 (d, 2H, J=6.2 Hz, Ho pyr); 7.31 (d, 2H, J=8.5 Hz, Hm Ar); 7.06 (d, 2H, J=8.5 Hz, Ho pyr); 5.35 (s, 2H, CH2Ar); 2.76 (t, 2H, J=7.6 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.74 (p, 2H, J=7.6 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.27 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.85 (m, 3H, CH3). 13C-NMR (CDCl3) δ: 165.1 C3; 152.8 C5; 150.5 Cm pyr; 135.4 Cipso pyr; 134.2 Cipso Ar; 134.0 Cp Ar; 129.3 Cm Ar; 128.0 Co Ar; 122.5 Co pyr; 52.1 CH2Ar; 31.7 CH2CH2CH2CH2CH2CH2CH3; 29.3 CH2CH2CH2CH2CH2CH2CH3; 29.2 CH2CH2CH2CH2CH2CH2CH3; 28.4 and 29.0CH2CH2CH2CH2CH2CH2CH3; 22.6 CH2CH2CH2CH2CH2CH2CH3; 14.1 CH3. MS (ES+)m/z: 369 (100%) [M+H]+.4-[1-(2,4-Dichlorobenzyl)-5-heptyl-1H-1,2,4-triazol-3-yl]pyridine (
15a
) and 4-[1-(2,4-Dichlorobenzyl)-3-heptyl-1H-1,2,4-triazol-5-yl]pyridine (
15b
). Compounds15a and15b were prepared from5 (200 mg, 0.8 mmol), 2,4-dichlorobenzyl chloride (114 μL, 0.8 mmol), and (Bu)4NBr (12 mg, 0.04 mmol.); reaction time: 2 h. Yield: 118 mg of15a (36%) as a white solid and 7 mg of15b (2%) as a yellow oil.15a: Mp = 104-105°C. 1H-NMR (CDCl3) δ: 8.65 (d, 2H, J=6.1 Hz, Hm pyr); 7.93 (d, 2H, J=6.1 Hz, Ho pyr); 7.42 (d, 1H, J=2.2 Hz, Hm′ Ar); 7.19 (dd, 1H, J=8.4 Hz and 2.2 Hz, Hm Ar); 6.58 (d, 1H, J=8.4 Hz, Ho Ar); 5.39 (s, 2H, CH2Ar); 2.72 (t, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.70 (p, 2H, J=7.7 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.23 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.84 (bt, 3H, J=6.5 Hz, CH3). 13C-NMR (CDCl3) δ: 159.3 C3; 158.1 C5; 150.3 Cm pyr; 138.3 Cipso pyr; 134.8 Cipso Ar; 133.0 Cp Ar; 131.7 Co′ Ar; 129.5 Co and Cm′ Ar; 127.8 Cm Ar; 120.4 Co pyr; 48.9 (CH2Ar); 31.6 CH2CH2CH2CH2CH2CH2CH3; 29.1 CH2CH2CH2CH2CH2CH2CH3; 28.8 CH2CH2CH2CH2CH2CH2CH3; 27.7 CH2CH2CH2CH2CH2CH2CH3; 26.0CH2CH2CH2CH2CH2CH2CH3; 22.5 CH2CH2CH2CH2CH2CH2CH3; 14.0 CH3. MS (ES+)m/z: 403 (100%) [M+H]+. Anal (C21H24Cl2N4) % calculated (% found) C: 62.53 (62.28); H: 6.00 (6.30); N: 13.89 (14.04).15b: 1H-NMR (CDCl3) δ: 8.70 (d, 2H, J=6.1 Hz, Hm pyr); 7.43 (d, 1H, J=2.2 Hz, Hm′ Ar); 7.41 (d, 2H, J=6.1 Hz, Ho pyr); 7.19 (dd, 1H, J=8.1 Hz and 2.2 Hz, Hm Ar); 6.85 (d, 1H, J=8.1 Hz, Ho Ar); 5.44 (s, 2H, CH2Ar); 2.77 (t, 2H, J=7.6 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.73 (p, 2H, J=7.6 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.25 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.85 (ta, 3H, J=6.7 Hz, CH3). 13C-NMR (CDCl3) δ: 165.5 C3; 152.8 C5; 150.6 Cm pyr; 138.3 Cipso pyr; 135.1 Cipso Ar; 132.8 Cp Ar; 131.9 (Co′ Ar); 129.7 Co Ar; 128.8 Cm′ Ar; 127.8 Cm Ar; 122.3 Co pyr; 50.1 CH2Ar; 31.6 CH2CH2CH2CH2CH2CH2CH3; 29.3 CH2CH2CH2CH2CH2CH2CH3; 29.0 CH2CH2CH2CH2CH2CH2CH3; 28.3CH2CH2CH2CH2CH2CH2CH3; 22.6 CH2CH2CH2CH2CH2CH2CH3; 14.1 CH3. MS (ES+)m/z: 403 (100%) [M+H]+. HPLC: Acetonitrile/H2O 90 : 10, tR=30.3 min (90% purity).


## 2.1.5. General Procedure for the Synthesis of16–18

To a solution of the corresponding triazole (1 equiv) in dry CH2Cl2 (4–10 mL), excess of MeI was added. The reaction mixture was stirred at room temperature for the time indicated. Afterwards, solvent was removedin vacuo and the residue was purified by chromatography or recrystallization from Et2O/CH2Cl2.4-(1-Benzyl-5-heptyl-1H-1,2,4-triazol-3-yl)-1-methylpyridinium Iodide (
16
). Compound16 was prepared from13a (15 mg, 0.05 mmol) and MeI (4 μL, 0.07 mmol); reaction time: 16 h. Purification: flash chromatography [CH2Cl2/MeOH (99 : 1) → CH2Cl2/MeOH (9 : 1)]. Yield: 14 mg of16 (66%) as a yellow gummy solid. 1H-NMR (CDCl3) δ: 9.18 (d, 2H, J=6.7 Hz, Hm pyr); 8.54 (d, 2H, J=6.7 Hz, Ho pyr); 7.35 (m, 3H, Ph); 7.19 (m, 2H, Ph); 5.36 (s, 2H, CH2Ar); 4.68 (s, 3H, NMe); 2.71 (t, 2H, J=7.6 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.67 (p, 2H, J=7.6 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.23 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.85 (bt, 3H, J=7.0 Hz, CH3). 13C-NMR (CDCl3) δ: 159.0 C3; 155.3 C5; 146.7 Cipso pyr; 145.6 Cm pyr; 134.3 Cipso Ph; 129.1 Cm Ph; 128.6 Cp Ph; 127.3 Co Ph; 123.7 Co pyr; 53.0 CH2Ar; 49.1 NMe; 31.5 CH2CH2CH2CH2CH2CH2CH3; 29.0 CH2CH2CH2CH2CH2CH2CH3; 28.8 CH2CH2CH2CH2CH2CH2CH3; 27.2 CH2CH2CH2CH2CH2CH2CH3; 26.1CH2CH2CH2CH2CH2CH2CH3; 22.5 CH2CH2CH2CH2CH2CH2CH3; 14.0 CH3. MS (ES+)m/z: 349 (100%), [M]+. Anal (C22H29IN4) % calculated (% found) C: 55.47 (55.42); H: 6.14 (6.30); N: 11.76 (11.57).4-[1-(4-Chlorobenzyl)-5-heptyl-1H-1,2,4-triazol-3-yl]-1-methylpyridinium Iodide (
17
). Compound17 was prepared from14a (70 mg, 0.2 mmol) and MeI (140 μL 2.3 mmol); reaction time: 8 days. Purification: flash chromatography [CH2Cl2/MeOH (95 : 5)]. Yield: 87 mg of17 (90%) as a yellow gummy solid. 1H-NMR (CDCl3) δ: 9.21 (d, 2H, J=6.8 Hz, Hm pyr); 8.54 (d, 2H, J=6.8 Hz, Ho pyr); 7.33 (d, 2H, J=8.5 Hz, Hm Ar); 7.15 (d, 2H, J=8.5 Hz, Ho Ar); 5.33 (s, 2H, CH2Ar); 4.68 (s, 3H, NMe); 2.71 (t, 2H, J=7.5 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.69 (p, 2H, J=7.5 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.23 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.85 (bt, 3H, J=6.5 Hz, CH3). 13C-NMR (CDCl3) δ: 158.9 C3; 155.4 C5; 146.3 Cipso pyr; 145.7 Cm pyr; 134.4 Cipso Ar; 132.7 Cp Ar; 129.1 Cm Ar; 128.7 Co Ar; 123.5 Co pyr; 52.1 CH2Ar; 48.9 NMe; 31.4 CH2CH2CH2CH2CH2CH2CH3; 28.9 CH2CH2CH2CH2CH2CH2CH3; 28.6 CH2CH2CH2CH2CH2CH2CH3; 27.0 CH2CH2CH2CH2CH2CH2CH3; 25.9CH2CH2CH2CH2CH2CH2CH3; 22.4 CH2CH2CH2CH2CH2CH2CH3; 13.9 CH3. MS (ES+)m/z: 383 (100%) [M]+. Anal (C22H28ClIN4) % calculated (% found) C: 51.73 (51.58); H: 5.52 (5.41); N: 10.97 (10.72).4-[1-(2,4-Dichlorobenzyl)-5-heptyl-1H-1,2,4-triazol-3-yl]-1-methylpyridinium Iodide (
18
). Compound18 was prepared from15a (50 mg, 0.1 mmol) and MeI (277 μL 4.45 mmol); reaction time: 8 days. Purification: recrystallization from Et2O/CH2Cl2. Yield: 43 mg of18 (64%) as a yellow solid. Mp = 136–138°C. 1H-NMR (CDCl3) δ: 9.22 (d, 2H, J=6.7 Hz, Hm pyr); 8.53 (d, 2H, J=6.7 Hz, Ho pyr); 7.44 (d, 1H, J=1.9 Hz, Hm′ Ar); 7.25 (dd, 1H, J=8.4 Hz and 1.9 Hz, Hm Ar); 7.00 (d, 1H, J=8.4 Hz, Ho Ar); 5.42 (s, 2H, CH2Ar); 4.69 (s, 3H, NMe); 2.76 (t, 2H, J=7.6 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.72 (p, 2H, J=7.6 Hz, CH2CH2CH2CH2CH2CH2CH3); 1.26 (m, 8H, CH2CH2CH2CH2CH2CH2CH3); 0.86 (bt, 3H, J=7.3 Hz, CH3). 13C-NMR (CDCl3) δ: 159.4 C3; 155.8 C5; 146.5 Cipso pyr; 145.7 Cm pyr; 135.4 Cipso Ar; 133.4 Cp Ar; 130.6 Co′ Ar; 130.3 Co Ar; 129.7 Cm′ Ar; 128,0 (Cm BnAr); 123,7 (Co CAr); 49,5 (CH2Ar); 49,2 (NMe); 31.5 CH2CH2CH2CH2CH2CH2CH3; 29.0 CH2CH2CH2CH2CH2CH2CH3; 28.8 CH2CH2CH2CH2CH2CH2CH3; 27.3 CH2CH2CH2CH2CH2CH2CH3; 25.9CH2CH2CH2CH2CH2CH2CH3; 22.5 CH2CH2CH2CH2CH2CH2CH3; 14.0 CH3. MS (ES+)m/z: 417 (100%) [M]+. Anal (C22H27Cl2IN4) % calculated (% found) C: 48.46 (48.71); H: 4.99 (5.20); N: 10.27 (10.06).


## 2.2. Pharmacology

Radioligand Binding Assays. CB1R binding assays in rat cerebellar membranes were performed using [3H]-SR141716A and [3H]-WIN552122 (NEN-Dupont, Boston, MA, 40–60 Ci/mmol) as radioligands, using the previously described methods [14]. Ki were calculated from the equation of Yung-Chi and Prusoff [15], using fixed Kd values for either [3H]-WIN552122 (8 nM) or [3H]-SR141716A (4 nM) obtained from independent experimental assays.


## 3. Results and Discussion

### 3.1. Chemistry

5-Aryl-3-heptyl-1H-1,2,4-triazoles were first synthesized, and then they were alkylated with different benzyl halide reagents. Preparation of disubstituted triazoles3–5 is depicted in Scheme 2. In the first step, 4-chlorobenzonitrile and 4-cyanopyridine reacted successively with sodium methoxide and ammonium chloride under inert conditions to afford amidinium hydrochlorides1 and2, respectively. Triazoles3–5 were obtained from1,2 and the commercially available benzamidine hydrochloride in moderate yields by refluxing them with octanoic hydrazide under basic conditions. Cyclization of 4-amidinopyridinium hydrochloride (2) was incomplete and the addition intermediate6 was allowed to be isolated. Acylamidrazone6 was then cyclized to5 under the same basic conditions (Scheme 2).Scheme 2
Reagents: (i) NaOMe, dry EtOH, N2atm; (ii) NH4Cl, N2atm; (iii) octanoic hydrazide, NaOMe, dry EtOH, Δ; and (iv) NaOMe, dry EtOH, Δ.The second step took place with the alkylation of triazoles3–5 under phase transfer catalysis conditions, using an aqueous sodium hydroxide solution as base and toluene as organic solvent [16]. These conditions were chosen after unsuccessful attempts of alkylation in an organic solvent (tetrahydrofuran) with mild (sodium bicarbonate) or strong (sodium hydride) bases. As depicted in Scheme 3, reaction of3–5 with different benzyl halides in the presence of tetrabutylammonium bromide yielded two products by alkylation on N2 (7a–15a) or N1 (7b–15b) of the triazole. Alkylation on N4 of the triazole was not detected, since its formation is hindered by steric reasons. Both alkylated isomers were easily isolated by chromatography, being the N2-benzyl derivatives obtained in greater proportion (≈10 : 1). The only N1 isomer that could not be isolated and characterized was13b; however it was detected by HPLC during the synthesis of13a. Higher ratio of N2 isomers was obtained by alkylation of5 with 4-chlorobenzyl and 2,4-dichlorobenzyl chlorides that led to a mixture of N2/N1 isomers in proportion of 13 : 1 and 18 : 1, respectively. These results support the fact that alkylation of 1,2,4-triazoles with benzyl halides is governed by steric reasons.Scheme 3
Reagents: (i) benzyl bromide for7a-7b,10a-10b, and13a; 4-chlorobenzyl chloride for8a-8b,11a-11b, and14a-14b; and 2,4-dichlorobenzyl chloride for9a-9b,12a-12b, and15a-15b; 40% NaOH aq, Bu4NBr, and toluene, 80°C.Since compounds7–15 are very lipophilic, pyridinium salts (16–18; Scheme 4) of some of the triazolylpyridines previously obtained were synthesized in order to test if they possessed improved aqueous solubility compared to the parent compounds. Increasing the aqueous solubility was important to perform the radioligand binding assays of the series of benzyl triazoles. Therefore, compounds13a–15a readily reacted with an excess of methyl iodide (1.5 equiv for13a, 11 equiv for14a, and 44 equiv for15a). Achievement of the triazolyl-1-methyl pyridinium salts needed long reaction times (16 h for16 and 8 days for17 and18), but the products were obtained in good yields.Scheme 4
Reagents: (i) MeI (excess), CH2Cl2, rt.Qualitative solubility tests of compounds16–18 did not show any improvement in their solubility in water; therefore they were not assessed by pharmacological assays.


### 3.2. Radioligand Binding Assays

Competitive radioligand binding assays have been used to evaluate the affinity of selected synthetized triazoles to CB1R in rat cerebellar membranes. They have been performed with [3H]-SR141716A and [3H]-WIN552122 as labelled ligands. The results of these preliminary assays are reported in Table 1.Table 1
Affinity of compounds7a-8aand10a–12a and the reference cannabinoids SR141716 and LH21 for CB1R determined using rat cerebellar membranes and [3H]-SR141716 or [3H]-WIN552122 as radioligand. Ki values were obtained from three independent experiments carried out in triplicate and are expressed as mean ± standard error.
Compound
K
i (nM) CB1R versus [3H]-SR141617
K
i (nM) CB1R versus [3H]-WIN552122
SR141716
K
d
=
0.59
4
LH21
855.6
±
296
748
±
193
7a
436
±
120
477
±
94
8a
589
±
136
561
±
125
10a
389.5
±
180
2437
±
888
11a
562
±
183
720
±
165
12a
13.9
±
2.4
323
±
60.5Compound12a showed high CB1R affinity versus [3H]-SR141617 (Ki=13.9 nM) and moderate affinity versus [3H]-WIN552122 (Ki=323 nM). These binding data indicate that12a displaced better SR141617, an inverse agonist of CB1R, than WIN552122, an agonist of CB1R. Since both SR141716 and WIN552122 have been reported in the literature to bind to CB1R in the same binding pocket [17], the results obtained here suggest that12a binds to the inactive state of CB1R, as the inverse agonists do (e.g., SR141716), and not to the active state of the receptor, as the agonists do (e.g., WIN552122) [18].The other tested compounds7a,8a,10a, and11a showed moderate CB1R affinity with affinity constant values in the low micromolar range.In what refers to the binding to CB2R, none of the compounds showed significant affinity using [3H]-CP55940 as radioligand in membranes purified from cells transfected with human CB2R (data not shown).


## 3.1. Chemistry

5-Aryl-3-heptyl-1H-1,2,4-triazoles were first synthesized, and then they were alkylated with different benzyl halide reagents. Preparation of disubstituted triazoles3–5 is depicted in Scheme 2. In the first step, 4-chlorobenzonitrile and 4-cyanopyridine reacted successively with sodium methoxide and ammonium chloride under inert conditions to afford amidinium hydrochlorides1 and2, respectively. Triazoles3–5 were obtained from1,2 and the commercially available benzamidine hydrochloride in moderate yields by refluxing them with octanoic hydrazide under basic conditions. Cyclization of 4-amidinopyridinium hydrochloride (2) was incomplete and the addition intermediate6 was allowed to be isolated. Acylamidrazone6 was then cyclized to5 under the same basic conditions (Scheme 2).Scheme 2
Reagents: (i) NaOMe, dry EtOH, N2atm; (ii) NH4Cl, N2atm; (iii) octanoic hydrazide, NaOMe, dry EtOH, Δ; and (iv) NaOMe, dry EtOH, Δ.The second step took place with the alkylation of triazoles3–5 under phase transfer catalysis conditions, using an aqueous sodium hydroxide solution as base and toluene as organic solvent [16]. These conditions were chosen after unsuccessful attempts of alkylation in an organic solvent (tetrahydrofuran) with mild (sodium bicarbonate) or strong (sodium hydride) bases. As depicted in Scheme 3, reaction of3–5 with different benzyl halides in the presence of tetrabutylammonium bromide yielded two products by alkylation on N2 (7a–15a) or N1 (7b–15b) of the triazole. Alkylation on N4 of the triazole was not detected, since its formation is hindered by steric reasons. Both alkylated isomers were easily isolated by chromatography, being the N2-benzyl derivatives obtained in greater proportion (≈10 : 1). The only N1 isomer that could not be isolated and characterized was13b; however it was detected by HPLC during the synthesis of13a. Higher ratio of N2 isomers was obtained by alkylation of5 with 4-chlorobenzyl and 2,4-dichlorobenzyl chlorides that led to a mixture of N2/N1 isomers in proportion of 13 : 1 and 18 : 1, respectively. These results support the fact that alkylation of 1,2,4-triazoles with benzyl halides is governed by steric reasons.Scheme 3
Reagents: (i) benzyl bromide for7a-7b,10a-10b, and13a; 4-chlorobenzyl chloride for8a-8b,11a-11b, and14a-14b; and 2,4-dichlorobenzyl chloride for9a-9b,12a-12b, and15a-15b; 40% NaOH aq, Bu4NBr, and toluene, 80°C.Since compounds7–15 are very lipophilic, pyridinium salts (16–18; Scheme 4) of some of the triazolylpyridines previously obtained were synthesized in order to test if they possessed improved aqueous solubility compared to the parent compounds. Increasing the aqueous solubility was important to perform the radioligand binding assays of the series of benzyl triazoles. Therefore, compounds13a–15a readily reacted with an excess of methyl iodide (1.5 equiv for13a, 11 equiv for14a, and 44 equiv for15a). Achievement of the triazolyl-1-methyl pyridinium salts needed long reaction times (16 h for16 and 8 days for17 and18), but the products were obtained in good yields.Scheme 4
Reagents: (i) MeI (excess), CH2Cl2, rt.Qualitative solubility tests of compounds16–18 did not show any improvement in their solubility in water; therefore they were not assessed by pharmacological assays.


## 3.2. Radioligand Binding Assays

Competitive radioligand binding assays have been used to evaluate the affinity of selected synthetized triazoles to CB1R in rat cerebellar membranes. They have been performed with [3H]-SR141716A and [3H]-WIN552122 as labelled ligands. The results of these preliminary assays are reported in Table 1.Table 1
Affinity of compounds7a-8aand10a–12a and the reference cannabinoids SR141716 and LH21 for CB1R determined using rat cerebellar membranes and [3H]-SR141716 or [3H]-WIN552122 as radioligand. Ki values were obtained from three independent experiments carried out in triplicate and are expressed as mean ± standard error.
Compound
K
i (nM) CB1R versus [3H]-SR141617
K
i (nM) CB1R versus [3H]-WIN552122
SR141716
K
d
=
0.59
4
LH21
855.6
±
296
748
±
193
7a
436
±
120
477
±
94
8a
589
±
136
561
±
125
10a
389.5
±
180
2437
±
888
11a
562
±
183
720
±
165
12a
13.9
±
2.4
323
±
60.5Compound12a showed high CB1R affinity versus [3H]-SR141617 (Ki=13.9 nM) and moderate affinity versus [3H]-WIN552122 (Ki=323 nM). These binding data indicate that12a displaced better SR141617, an inverse agonist of CB1R, than WIN552122, an agonist of CB1R. Since both SR141716 and WIN552122 have been reported in the literature to bind to CB1R in the same binding pocket [17], the results obtained here suggest that12a binds to the inactive state of CB1R, as the inverse agonists do (e.g., SR141716), and not to the active state of the receptor, as the agonists do (e.g., WIN552122) [18].The other tested compounds7a,8a,10a, and11a showed moderate CB1R affinity with affinity constant values in the low micromolar range.In what refers to the binding to CB2R, none of the compounds showed significant affinity using [3H]-CP55940 as radioligand in membranes purified from cells transfected with human CB2R (data not shown).


## 4. Conclusions

In our ongoing program searching for novel cannabinoid ligands, we reported a CB1R antagonist [5-(4-chlorophenyl)-1-(2,4-dichlorophenyl)-3-hexyl-1H-1,2,4-triazole, LH21], which showed an interestingin vitro andin vivo pharmacological profile and was able to reduce food intake and body weight in obese animals with major peripheral components. In the present study, we have explored structural modifications on this 1,2,4-triazole scaffold. A series of new 3(5)-alkyl-5(3)-aryl-1-benzyl-1H-1,2,4-triazoles were synthesized and competitive binding assays of selected compounds were carried out. One of these triazoles (12a) showed high affinity for CB1R.



---

*Source: 1257098-2016-03-31.xml*