# The GenusLuehea (Malvaceae-Tiliaceae): Review about Chemical and Pharmacological Aspects

Authors: João Tavares Calixto-Júnior; Selene Maia de Morais; Aracélio Viana Colares; Henrique Douglas Melo Coutinho
Journal: Journal of Pharmaceutics
 (2016)

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## Abstract

Popularly known as “açoita-cavalo” (whips-horse),Luehea species (Malvaceae-Tilioideae) are native to America and are used in folk medicine as anti-inflammatory, antidiarrheal, antiseptic, expectorant, and depurative and against skin infections. Although there are studies showing the chemical constituents of some species, the active substances have not been properly identified. A systematic study was carried out through a computer search of data on CAPES journals, SciELO, ISI Bireme, PubMed, ScienceDirect, ScienceDomain Medline, and Google Scholar from published articles using key words:Luehea, açoita-cavalo, and Malvaceae.Luehea divaricata was the species with the highest number of studies observed. Triterpenes (9), flavonoids (6), and steroids (4), including saponins, organic acids (4), and one lignan, are the main types of secondary metabolites registered and the most cited flavonoids were rutin and quercetin and among triterpenes there was maslinic acid, which might be associated with the popular indication of its anti-inflammatory action. The vitexin, a C-glycosylated flavone, isolated from three different species, is cited as a possible taxonomic marker of the genus. Studies confirm in part the medicinal uses of plants named as “açoita-cavalo” species. Some pharmacological activities, not assigned to the species of the genusLuehea by populations, were observed in laboratory experiments.


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## Body

## 1. Introduction

Malvaceae is a family consisting of herbs, subshrubs, shrubs, lianas, and small and large trees, with about 250 genera and 4,200 species, of which, in Brazil, about 80 genera and 400 species are found [1]. According to the List of Species of Flora of Brazil, 69 genera and 754 species are indicated, with 30 genera distributed in 393 taxa of the subfamily Malvoideae [2, 3].Luehea genus, which belongs to Malvaceae family, is essentially neotropical, existing in southern Mexico, including the West Indies to Uruguay and Argentina. Currently there are about 25 species and 3 varieties, of which 12 species and one variety exist in Brazil, with its highest concentration in the southeast and midwest regions [4]. The genusLuehea is present in Brazilian Cerrado and thirteen species are registered in Brazilian herbariums:Luehea altheaeflora Spruce ex Benth.,Luehea fiebrigii Burret,Luehea candicans Mart.,Luehea candida (Moc. & Sessé ex DC.) Mart.,Luehea crispa Krapov.,Luehea cymulosa Spruce ex Benth.,Luehea divaricata Martius et Zuccarini,Luehea grandiflora Mart. & Zucc.,Luehea paniculata Mart. & Zucc.,Luehea ochrophylla Mart.,Luehea rosea Ducke andLuehea speciosa Willd., andL. conwentzii K. Schum.The lectotype ofL. conwentzii was selected and a new synonym was proposed—L. eichler Schum [5]. To Rizzini and Mors [6], the various species are very similar to each other, getting the same common names (horse-whips) and having identical uses.Among the species of this genus, the most cited areL. grandiflora andL. divaricate, popularly known as “açoita-cavalo” (whips-horse) [7]. The closest species ofL. divaricata isL. paniculata, which is a tree a little lower, existing in Bolivia, Paraguay, Peru, and several states of Brazil [8]. The herbarium specimens ofL. grandiflora showed close similarities to those ofL. speciosa because they are both very polymorphic. However,L. speciosa staminodia are profoundly fimbriated, while those ofL. grandiflora are slightly fimbriated in the apex. SoL. grandiflora was considered a synonym ofL. speciosa but was later rehabilitated [9].ALuehea herbal product, composed of dried leaves, is used against dysentery, leucorrhoea, rheumatism, gonorrhea, and tumors; infusion of the flowers is used against bronchitis and the root is depurative [10]. Aerial parts ofL. divaricata are used popularly for skin wounds and for intimate hygiene [11]. Backes and Irgang [12] reported the use of the bark of this plant as antirheumatic, antidiarrheal, antiseptic, expectorant, and cleanser.The current state of knowledge of the chemistry and pharmacology of genusLuehea indicates its potential for developing anti-inflammatory drugs and antibiotics; however, few studies with species of this genus were reported.Due to the importance of the genusLuehea, as a source of new medicinal agents, a review is worthwhile. The data may support future multidisciplinary studies involving phytochemical studies of the genus species to perform a critical analysis of their use by populations with a view to the preservation of plant species in their respective biomes and promoting rational use of “açoita-cavalo” as a therapeutic resource. A systematic study was carried out through a computer search of data on CAPES journals, SciELO, ISI Bireme, PubMed, ScienceDirect, ScienceDomain Medline, and Google Scholar from published articles using key words:Luehea, açoita-cavalo, and Malvaceae. Data was also obtained from theses, proceedings, and book of abstracts and reviews indexed in the databases, used for this work, as well as abstracts and full papers published in scientific events. Dissertations and doctoral theses of Brazilian and international students with relevant data were also examined. Data presented include scientific name and activities of the plants.


## 2. Development

### 2.1. Chemical Aspects

#### 2.1.1.Luehea divaricata

Little information is known about the chemical constituents present in the genusLuehea [8]. The phytochemical analysis of the leaves of this species showed mainly the presence of flavonoids, saponins, and catechin tannins (condensed tannins) [13]. To a lesser extent, the authors cited the presence of alkaloids, fixed oils, anthocyanins, carotenoids, and polysaccharides. The species presents tannins, essential oil, resin, and mucilage [14].Portal et al. [15], conducting a phytochemical screening on the extract ofL. divaricata collected in Belém, Pará State, Northern Brazil, noted the presence in the leaves of reducing sugars, proteins, amino acids, tannins, catechins, flavonoids, carotenoids, steroids, triterpenoids, and saponins. Several authors performed a phytochemical screening with ethanol extracts of leaf and stem and also observed the presence of flavonoids, tannins, saponins, and triterpenes/steroids [16–18] and Bertucci et al. [19] screening native species of the Uruguay River observed in the hydroalcoholic extract of leaves ofL. divaricata the presence of these same constituents.Lopes [18], through phytochemical study ofL. divaricata collected in Southern Brazil, identified from the alcoholic extract of leaves, tannins, saponins, and flavonoids and isolated by two-dimensional thin-layer chromatography the flavonoids quercetin (1), rutin (2), and vitexin (3) (Figure 1).Figure 1
Phytocompounds isolated fromLuehea divaricata.Vargas et al. [20] also point out the presence of tannins, flavonoids, and saponins in leaves and bark ofL. divaricata and also the presence of quercetin (1) and kaempferol (4) in the extracts. However, Maraschin-Silva and Aqüila [21], investigating the allelopathic potential of this species, only infer about the presence of tannins and saponins in aqueous leaf extract.Arantes [17] analyzed the ethanol extract of leaves collected in Southern Brazil by HPLC and reported the presence of the flavonoids quercetin (1), rutin (2), and kaempferol (4) and aromatic acids gallic acid (5), chlorogenic acid (6), and caffeic acid (7) (Figure 1).Tanaka et al. [22] isolated from the methanol extract of leaves ofL. divaricata, collected in Maringá, Paraná State, Southern Brazil, a new triterpene (basic skeleton of ursene): 3β-p-hydroxybenzoyloxytormentic acid [3β-(p-hydroxybenzoyloxy)-2α-hydroxyurs-12-en-28-oic acid] (8), a mixture of tormentic acid ester glucoside (9), tormentic acid (10), and the maslinic acid (olean-12-ene-2α,3β-diol) (11) (basic skeleton of oleanane) (Figure 1).Tanaka et al. [10], in continuation of previous work, isolated from stem bark and leaves, besides the mentioned triterpenes, the steroid glucopyranosylsitosterol (12), (−)-epicatechin (13), a flavonoid which belongs to a class of flavan-3-ol, and vitexin (3), as already indicated in the same species isolated in previous work [18] (Figure 1).The presence of flavonoids such as vitexin (3) [23, 24] and triterpenes as maslinic acid (11) [25, 26] may be associated with the popular indication of its anti-inflammatory action [27].Besides polyphenols (flavonoids, catechins, anthocyanins, and tannins) the phytochemical screening of the hydroalcoholic extract of bark ofL. divaricata collected in Leme, São Paulo State, Southeastern Brazil, identified the presence of saponins, triterpenes, steroids, and anthracenes [28]. Walker et al. [29], in histochemical and morphoanatomical study of leaves ofL. divaricata Mart., reported about the presence of mucilage and calcium oxalate in the form of a prism and drusen in idioblasts.Luehea ochrophylla. Two papers that focus on the phytochemical analysis of this species are listed in this review. The phytochemical study of skins ofL. ochrophylla collected in Grão Mogol, Minas Gerais State, Southeastern Brazil, resulted in the isolation of hydrocarbons, aliphatic esters of steroid β-sitosterol (14), and pentacyclic triterpenes friedelin (15) and β-friedelinol (16) [30] (Figure 2).Figure 2
Phytocompounds isolated fromLuehea ochrophylla.


#### 2.1.2.Luehea grandiflora

The works of da Silva et al. [30] that indicate polyphenols in the cortex ofL. grandiflora and Rosa et al. [31, 32] were the only works with focus on the phytochemistry of this species. Rosa et al. [31, 32] working with crude ethanol extract of leaves collected in Goiás State, Brazil, observed, from fractionation by column chromatography isolation, in chloroform fraction, the lupeol triterpene (16) and the mixture of steroids β-sitosterol (14), stigmasterol (17), and campesterol (18) (Figure 3).Figure 3
Phytocompounds isolated fromLuehea grandiflora.


#### 2.1.3.Luehea candida

The anticancer potential ofL. candida extracts was evaluated against many cell lines and lupeol (16), betulin (19), (−)-epicatechin (13), vitexin (3), and a lignan liriodendrin (20) were isolated from the active fractions [8] (Figure 4).Figure 4
Phytocompounds isolated fromLuehea candida.Saénz and Nassar [33] established the presence, in small quantities, of alkaloids in this species. Alkaloids were also pointed out in qualitative studies onLuehea seemannii collected in Eastern Nicaragua [34] and leaves and bark in this species collected in Villa Neily, Costa Rica [35, 36].


#### 2.1.4.Luehea paniculata

Barbosa and Reed [35, 36] analyzing ethanol extracts ofL. grandiflora andL. paniculata collected in Goiania, Brazil, inferred about the presence of tannins and flavonoids in leaves and bark of both species. The existence of rutin (2) inL. paniculata was identified by means of thin-layer chromatography.Calixto Júnior et al. [37] observed by HPLC-DAD fingerprinting inL. paniculata ethanolic extracts (leaves and sapwood) the presence of the gallic acid (5), chlorogenic acid (6), and rosmarinic acid (21) and flavonoids rutin (2), vitexin (3), and luteolin (22) (Figure 5).Figure 5
Phytocompounds isolated fromLuehea paniculata.Alves et al. [38] reported the isolation of triterpenes maslinic acid (11) from the chloroform fraction and oleanolic acid (23) and lupenone (24) from the ethyl acetate fraction, as well as evaluating the antiproliferative activity of the crude extract fractions of ethyl acetate and hydromethanol obtained from the leaves ofL. paniculata collected in the Cerrado, Central Brazil (Figure 5).


#### 2.1.5.Luehea candicans

Silva [39], in a study that involved extraction, isolation, and identification of the chemical constituents of the branches and leaves ofL. candicans in the Puerto Rico region, Paraná State, Southern Brazil, got the isolation, the crude methanol extract of the stems of two triterpenes belonging to the class of lupins, the lup-20(29)-en-3β-ol (lupeol) (16) and the betulin—3β,28-diidroxilup-20(29)-ene (19). The works of Silva [39] and Da Silva et al. [8] were the only ones found on chemical research in this species.The presence of vitexin in the leaves ofL. candicans,L. divaricata, andL. paniculata species can suggest the hypothesis that this flavone may be a possible taxonomic marker of the genusLuehea.


### 2.2. Ethnopharmacological and Pharmacological Aspects

The popular knowledge about the medicinal uses of horse-whips is reported by several authors [40]; however, there are few studies available on the pharmacological potential of the species [12]. The highest number of citations on ethnopharmacological information is assigned to the speciesL. divaricata; however, some other species appear in other surveys.Luehea seemannii is popularly used against bites and stings (snake, scorpion, and insects) and also as an astringent in Eastern Nicaragua [34].In a survey conducted about ethnopharmacology of medicinal plants of the Pantanal (Mato Grosso State, Brazil),L. divaricata is among the species with the highest relative importance value (1.50) between values ranging from 0.17 to 1.87, among the 261 species mentioned in the work [27]. The authors report citations, by respondents, about the use ofL. divaricata in the treatment of lung and upper respiratory disease. However, they stress that no scientific evidence exist on the activity of the species in the regulation of cough, while its antibiotic properties also vary. Bessa et al. [41] reported a quote from “açoita-cavalo” as being of use to the treatment of ulcer in rural community of Green Vale Settlement, Tocantins State, Central Brazil.Several other reports of uses of this species were found: treatment of rheumatism; arthritis; dysentery; internal cleaning of wounds and ulcers; depurative; sore throat; painkiller for toothache; astringent; evils of the bladder; sleep balance; melena (intestinal colic followed by diarrhea with painful bowel movements and presence of blood in the stool); leucorrhea; gonorrhea; bleeding; cough; laryngitis; bronchitis; deworming; gastritis; poor digestion; diarrhea; and cancer and tumors [9, 42–44].Degen et al. [45] reported medicinal plants traded by their common names and cited “Francisco Álvarez” asL. divaricata being employed against diabetes in Argentina and in Paraguay, where the same common name matchesthe Banara arguta Brig. (Flacourtiaceae). The term “Francisco Álvarez” as reference to “açoita-cavalos” in Paraguay is also cited by Carvalho [46], which emphasizes the use of other common names abroad: azota caballo and árbol de San Francisco, Argentina; Francisco Álvarez, Uruguay; and ka’a oveti, Paraguay. In various regions of Brazil the author highlights açoita: açoita-cavalo-do-miúdo, açoita-cavalo-branco, ivantingui, and vatinga in the São Paulo State; açoita-cavalo in Paraná State; in the states of Rio de Janeiro and São Paulo, biatingui, erviteira-do-campo, estibeiro, and estriveira; in Bahia State and São Paulo State, guaxima-do-campo, ibatingui, and ivatingui; in Minas Gerais State, ivitinga; in Bahia State, ivitingui, luitingui, mutamba, and pau-de-canga; in Santa Catarina State, salta-cavalo; in Paraná State and São Paulo State, soita and soita-cavalo; and, in Paraná State, ubatinga.In folk medicine the “açoita-cavalo” is used in cases of dysentery, bleeding, arthritis, leucorrhoea, rheumatism, and tumors [41]. In ethnodirected work at Quilombo Sangrador, Maranhão State, Northeastern Brazil, the “açoita-cavalo” was one of the species with more valuable importance, with indications for the treatment of diseases of the genitourinary system [47]. Basualdo and Soria [48] includedL. divaricata Mart. in the list of medicinal plants of Paraguay used in fighting respiratory infections.The leaves ofLuehea are marketed as herbal against leucorrhoea, rheumatism, gonorrhea, and tumors; infusion of the flowers is used against bronchitis and the root is depurative [10]. Rai [11] reports that the aerial parts of “açoita-cavalo” (L. divaricata Martius et Zuccarini) are used in traditional medicines for skin wounds, grain cleaning, and vaginal washings. Maffei [49] in work on medicinal plants of Uruguay and Chiriani [50] in study about phytotherapy in Argentina inform that the barks are used as antipyretic and antianemic agents, in addition to antidiarrheal, astringent, and antitumor. Quotes on the leaves as herbal against rheumatism, dysentery, gonorrhea, soothing, and antispasmodic are observed in several works [10, 49, 51–53]. The root is pointed out as anti-inflammatory and cleansing by Alice et al. [54], and the flowers are cited, along with bark and leaves, with diuretic, antiarthritic, antileucorrhoea, and wound healing of the skin and vaginal washings [22, 40, 49, 55, 56]. The bath of the stem bark was quoted in ethnobotanical survey in the Upper Rio Grande, Minas Gerais State, Southeastern Brazil, [57] as being of use for dysentery, as antirheumatic and antihemorrhagic agent.According to Alice et al. [58], the bark of theL. divaricata is also used to combat the fever and is also suitable for gastrointestinal and liver disorders. The leaves are used as anti-inflammatory and employed in disorders of the respiratory tract and bronchitis. In popular phytotherapy the cortex of stems ofL. divaricata is used in decoctions and infusions by tonic and antidiarrheal characteristics [59]. The resin of the fruit is applied as antiodontalgic; 1% flowers infusion is used as sedative and the infusion of the leaves as anti-inflammatory [60, 61].


### 2.3. Microbiological Activities

Five studies have reported effects against microorganisms in this species. The antifungal activity ofL. divaricata was evaluated by Zacchino et al. [62]. As a result, the authors pointed out the moderate action of dichloromethane extract in inhibiting the growth of hyphae of some species of dermatophytes; however, this inhibitory effect is not observed for other fungal species [10, 40]. However, the extract ofL. divaricata showed strong inhibition of the growth ofStaphylococcus aureus,Staphylococcus epidermidis,Klebsiella pneumonia, andEscherichia coli in one study [40] but showed only moderate inhibition in another study and another collection site [10].Montovani et al. [63] also investigated antimicrobial action in extract ofL. divaricata. Collected in São Pedro do Iguaçu, Paraná State, Southern Brazil, the authors obtained extracts of leaves from three solvents (hexane, ethyl acetate, and ethanol) and diagnosed positive effects in inhibitingS. aureus,Bacillus cereus,E. coli, andSalmonella typhi. However, for the fungusAspergillus niger, they obtained negative result; Coelho De Souza et al. [64], as a result of ethnopharmacological study, evaluated the antimicrobial potential of some plants in common use in the Rio Grande do Sul State (Southern Brazil) using the agar diffusion method; the authors found that the methanol extract ofL. divaricata showed activity against theMicrococcus luteus bacteria. The extract showed no activity against other bacteria such asS. aureus,S. epidermidis, andE. coli, even against yeastCandida albicans.Marques et al. [65] reported the biological activities study ofL. paniculata of the Brazilian Cerrado, which showed inhibitory effect of ethanol extract ofL. paniculata leaves onS. aureus bacteria.Calixto Júnior et al. [37] pointed out as irrelevant the activity ofL. paniculata leaf ethanolic extract (LEELP) tested against six strains ofCandida (MIC ≥ 1024). This work is the first recorded piece of research on the potentialization of Fluconazole using extracts ofL. paniculata against three strains ofCandida, in particular,C. tropicalis andC. albicans, and there was, therefore, a synergism when the extracts were combined with Fluconazole.


### 2.4. Anti-Inflammation and Toxicity

Lopes [18] investigated the anti-inflammatory action ofL. divaricata. From an experiment using the aqueous extract obtained from the dried leaves of the plant, the authors used the test of rat paw edema induced by carrageenan. The extract was administered intraperitoneally at doses of 100 and 150 mg/kg and orally, using a stomach tube at a dose of 300 mg/kg. According to the authors, the dose of 150 mg/kg administered intraperitoneally obtained the peak reduction of the edema. Siqueira [28] claims to have the hydroalcoholic extract ofL. divaricata that reduced the rate of ulcerative lesions produced by indomethacin and ethanol. The author indicates that the mechanism of action antiulcerogenic is partly related to the activity of sulfhydryl radicals and by the precipitation of proteins produced by the presence of polyphenols present in the plant.Bianchi [66] conducted studies of acute and subacute toxicity with extracts of this species. In both tests Swiss mice, males, were used. The alcoholic extract administered intraperitoneally at doses of 250 mg/kg and 500 mg/kg triggered diarrhea and bristling fur, within 72 hours, that were observed in the acute toxicity test. 50% of deaths of the animals were seen using a dose of 500 mg/kg for 48 hours. In the subacute toxicity test, the authors administered a dose of 25 mg/kg of aqueous and alcoholic extract by means intraperitoneally, once a week for 8 days. The aqueous extract triggered the death of an animal after the 8th dose, unlike the alcoholic extract that at this dose did not cause any deaths [67]. Vargas et al. [20] showed genotoxic (mutagenic) activity in aqueous leaf extract ofL. divaricata in the Ames test (Salmonella/microsome) with microsomal activation. This fact can be explained by the presence of tannins and flavones in the leaves of the species that present patterns of hydroxylation, which could possibly cause damage to the DNA structure [54].The phytotoxic activity ofL. divaricata was shown clearly by Souza et al. [68]. The authors point out the test with aqueous extract of leaves collected in Pelotas, Southern Brazil, which showed an inhibitory effect on the germination ofLactuca sativa. Nevertheless,L. divaricata extracts demonstratein vivo lack of toxicity or mutagenicity [69, 70].


### 2.5. Antioxidant Potential

Good antioxidant activity and analgesic properties in leaves ofL. divaricata were pointed out by Müller [40]. By DPPH method, the author observed the crude extract and ethyl acetate and butanol fractions with similar antioxidant with the quercetin. Arantes [17] showed antioxidant activity at low concentrations of ethanol extract of the leaves of this species, observed by reducing basal levels of lipid peroxidation by the extract and its protective effect against lipid peroxidation and decreased cell viability induced by nitroprusside sodium in rat brainin vitro.Antioxidant effect of the ethanol extract ofLuehea paniculata roots was evaluated by the method of scavenging free radical DPPH [37]. This extract demonstrated an antioxidant potential almost similar to the value associated with the flavonoid quercetin (EC50 = 0.85 and 0.01 mg/mL, resp.). Calixto Júnior et al. [37] showed good results in antioxidant test of leaf extract and bark with IC50 values: 0.32 and 0.24 mg/mL, respectively.


### 2.6. Antiproliferative Activity

Alves et al. [38] analyzed the antiproliferative activity of the crude extract and ethyl acetate and hydromethanol fractions ofL. paniculata leaves. The evaluation was performed at concentrations from 0.25 to 250 mg/mL against three human tumor cell lines, breast (MCF-7), lung (NCI-H460), and glioma (U251), by the colorimetric method with sulforhodamine B. The results showed moderate antiproliferative potential for those fractions.The crude methanolic extracts of the branches and leaves ofLuehea candicans were evaluated using the following cancer cell lines: MCF-7 (breast), NCI-ADR (breast expressing the multidrug resistance phenotype), NCI-460 (lung), UACC-62 (melanoma), 786-0 (kidney), OVCAR (ovarian), PCO-3 (prostate), HT-29 (colon), and K-562 (leukaemia). The crude methanolic extracts from the branches (B) and leaves (L) were able to inhibit the growth of the K-562 and 786-0 cell lines in a dose-dependent manner, with GI50 values of 8.1 and 5.4 µg /mL, respectively. The hexane (L1), chloroform (L2), and methanol (L4) fractions derived from extract L showed a high selectivity and pronounced cytostatic activity against 786-0 (G
I
50
~40 µg /mL). A significant amount of lupeol was isolated from fraction L2. The chloroform (B2) and methanol (B3) fractions derived from extract (B) exhibited less selectivity, showing the highest cytostatic activity against K-562, NCI-ADR, OVCAR, MCF-7, and NCI-460 cells, with GI50 values between 27 and 40 µg/mL [8].Silva [39] studiedL. candicans in the region of Puerto Rico, Paraná State, Southern Brazil, and assessed, by biological tests, the antibacterial, antifungal, and antiproliferative crude extracts, fractions, and isolated compounds. None of the extracts, however, showed significant antibacterial activity; however, the crude extract of the leaves showed antifungal activity againstCandida krusei strain with a fungicide at a concentration of 125 mg/mL (CMF) and fungistatic concentration of 62.5 mg/mL (CIM). The crude extract ofL. candicans still showed antiproliferative activity with cytostatic effect of 85% (25 μg/mL) and cytocidal effect by 25%, the highest concentration used for cell kidney cancer. The hexane, CHCl3, and MeOH fractions demonstrated a cytostatic effect of 100% against kidney cancer cells. The crude extract of the stems showed cytostatic and antiproliferative activity with little selectivity among the studied strains (25% cell death at the highest concentration) effects, since the crude extract ofL. candicans and hexane, CHCl3 and MeOH fractions were selective for cells of kidney cancer exhibiting antiproliferative activity with cytostatic effect (growth inhibition) by 85% at a concentration of 25 mg/mL for the crude extract and 100% (250 μg/mL) for fractions. The antifungal activity of triterpenes, steroids, and flavonoids isolated was also tested, but without any substances presenting activity.


## 2.1. Chemical Aspects

### 2.1.1.Luehea divaricata

Little information is known about the chemical constituents present in the genusLuehea [8]. The phytochemical analysis of the leaves of this species showed mainly the presence of flavonoids, saponins, and catechin tannins (condensed tannins) [13]. To a lesser extent, the authors cited the presence of alkaloids, fixed oils, anthocyanins, carotenoids, and polysaccharides. The species presents tannins, essential oil, resin, and mucilage [14].Portal et al. [15], conducting a phytochemical screening on the extract ofL. divaricata collected in Belém, Pará State, Northern Brazil, noted the presence in the leaves of reducing sugars, proteins, amino acids, tannins, catechins, flavonoids, carotenoids, steroids, triterpenoids, and saponins. Several authors performed a phytochemical screening with ethanol extracts of leaf and stem and also observed the presence of flavonoids, tannins, saponins, and triterpenes/steroids [16–18] and Bertucci et al. [19] screening native species of the Uruguay River observed in the hydroalcoholic extract of leaves ofL. divaricata the presence of these same constituents.Lopes [18], through phytochemical study ofL. divaricata collected in Southern Brazil, identified from the alcoholic extract of leaves, tannins, saponins, and flavonoids and isolated by two-dimensional thin-layer chromatography the flavonoids quercetin (1), rutin (2), and vitexin (3) (Figure 1).Figure 1
Phytocompounds isolated fromLuehea divaricata.Vargas et al. [20] also point out the presence of tannins, flavonoids, and saponins in leaves and bark ofL. divaricata and also the presence of quercetin (1) and kaempferol (4) in the extracts. However, Maraschin-Silva and Aqüila [21], investigating the allelopathic potential of this species, only infer about the presence of tannins and saponins in aqueous leaf extract.Arantes [17] analyzed the ethanol extract of leaves collected in Southern Brazil by HPLC and reported the presence of the flavonoids quercetin (1), rutin (2), and kaempferol (4) and aromatic acids gallic acid (5), chlorogenic acid (6), and caffeic acid (7) (Figure 1).Tanaka et al. [22] isolated from the methanol extract of leaves ofL. divaricata, collected in Maringá, Paraná State, Southern Brazil, a new triterpene (basic skeleton of ursene): 3β-p-hydroxybenzoyloxytormentic acid [3β-(p-hydroxybenzoyloxy)-2α-hydroxyurs-12-en-28-oic acid] (8), a mixture of tormentic acid ester glucoside (9), tormentic acid (10), and the maslinic acid (olean-12-ene-2α,3β-diol) (11) (basic skeleton of oleanane) (Figure 1).Tanaka et al. [10], in continuation of previous work, isolated from stem bark and leaves, besides the mentioned triterpenes, the steroid glucopyranosylsitosterol (12), (−)-epicatechin (13), a flavonoid which belongs to a class of flavan-3-ol, and vitexin (3), as already indicated in the same species isolated in previous work [18] (Figure 1).The presence of flavonoids such as vitexin (3) [23, 24] and triterpenes as maslinic acid (11) [25, 26] may be associated with the popular indication of its anti-inflammatory action [27].Besides polyphenols (flavonoids, catechins, anthocyanins, and tannins) the phytochemical screening of the hydroalcoholic extract of bark ofL. divaricata collected in Leme, São Paulo State, Southeastern Brazil, identified the presence of saponins, triterpenes, steroids, and anthracenes [28]. Walker et al. [29], in histochemical and morphoanatomical study of leaves ofL. divaricata Mart., reported about the presence of mucilage and calcium oxalate in the form of a prism and drusen in idioblasts.Luehea ochrophylla. Two papers that focus on the phytochemical analysis of this species are listed in this review. The phytochemical study of skins ofL. ochrophylla collected in Grão Mogol, Minas Gerais State, Southeastern Brazil, resulted in the isolation of hydrocarbons, aliphatic esters of steroid β-sitosterol (14), and pentacyclic triterpenes friedelin (15) and β-friedelinol (16) [30] (Figure 2).Figure 2
Phytocompounds isolated fromLuehea ochrophylla.


### 2.1.2.Luehea grandiflora

The works of da Silva et al. [30] that indicate polyphenols in the cortex ofL. grandiflora and Rosa et al. [31, 32] were the only works with focus on the phytochemistry of this species. Rosa et al. [31, 32] working with crude ethanol extract of leaves collected in Goiás State, Brazil, observed, from fractionation by column chromatography isolation, in chloroform fraction, the lupeol triterpene (16) and the mixture of steroids β-sitosterol (14), stigmasterol (17), and campesterol (18) (Figure 3).Figure 3
Phytocompounds isolated fromLuehea grandiflora.


### 2.1.3.Luehea candida

The anticancer potential ofL. candida extracts was evaluated against many cell lines and lupeol (16), betulin (19), (−)-epicatechin (13), vitexin (3), and a lignan liriodendrin (20) were isolated from the active fractions [8] (Figure 4).Figure 4
Phytocompounds isolated fromLuehea candida.Saénz and Nassar [33] established the presence, in small quantities, of alkaloids in this species. Alkaloids were also pointed out in qualitative studies onLuehea seemannii collected in Eastern Nicaragua [34] and leaves and bark in this species collected in Villa Neily, Costa Rica [35, 36].


### 2.1.4.Luehea paniculata

Barbosa and Reed [35, 36] analyzing ethanol extracts ofL. grandiflora andL. paniculata collected in Goiania, Brazil, inferred about the presence of tannins and flavonoids in leaves and bark of both species. The existence of rutin (2) inL. paniculata was identified by means of thin-layer chromatography.Calixto Júnior et al. [37] observed by HPLC-DAD fingerprinting inL. paniculata ethanolic extracts (leaves and sapwood) the presence of the gallic acid (5), chlorogenic acid (6), and rosmarinic acid (21) and flavonoids rutin (2), vitexin (3), and luteolin (22) (Figure 5).Figure 5
Phytocompounds isolated fromLuehea paniculata.Alves et al. [38] reported the isolation of triterpenes maslinic acid (11) from the chloroform fraction and oleanolic acid (23) and lupenone (24) from the ethyl acetate fraction, as well as evaluating the antiproliferative activity of the crude extract fractions of ethyl acetate and hydromethanol obtained from the leaves ofL. paniculata collected in the Cerrado, Central Brazil (Figure 5).


### 2.1.5.Luehea candicans

Silva [39], in a study that involved extraction, isolation, and identification of the chemical constituents of the branches and leaves ofL. candicans in the Puerto Rico region, Paraná State, Southern Brazil, got the isolation, the crude methanol extract of the stems of two triterpenes belonging to the class of lupins, the lup-20(29)-en-3β-ol (lupeol) (16) and the betulin—3β,28-diidroxilup-20(29)-ene (19). The works of Silva [39] and Da Silva et al. [8] were the only ones found on chemical research in this species.The presence of vitexin in the leaves ofL. candicans,L. divaricata, andL. paniculata species can suggest the hypothesis that this flavone may be a possible taxonomic marker of the genusLuehea.


## 2.1.1.Luehea divaricata

Little information is known about the chemical constituents present in the genusLuehea [8]. The phytochemical analysis of the leaves of this species showed mainly the presence of flavonoids, saponins, and catechin tannins (condensed tannins) [13]. To a lesser extent, the authors cited the presence of alkaloids, fixed oils, anthocyanins, carotenoids, and polysaccharides. The species presents tannins, essential oil, resin, and mucilage [14].Portal et al. [15], conducting a phytochemical screening on the extract ofL. divaricata collected in Belém, Pará State, Northern Brazil, noted the presence in the leaves of reducing sugars, proteins, amino acids, tannins, catechins, flavonoids, carotenoids, steroids, triterpenoids, and saponins. Several authors performed a phytochemical screening with ethanol extracts of leaf and stem and also observed the presence of flavonoids, tannins, saponins, and triterpenes/steroids [16–18] and Bertucci et al. [19] screening native species of the Uruguay River observed in the hydroalcoholic extract of leaves ofL. divaricata the presence of these same constituents.Lopes [18], through phytochemical study ofL. divaricata collected in Southern Brazil, identified from the alcoholic extract of leaves, tannins, saponins, and flavonoids and isolated by two-dimensional thin-layer chromatography the flavonoids quercetin (1), rutin (2), and vitexin (3) (Figure 1).Figure 1
Phytocompounds isolated fromLuehea divaricata.Vargas et al. [20] also point out the presence of tannins, flavonoids, and saponins in leaves and bark ofL. divaricata and also the presence of quercetin (1) and kaempferol (4) in the extracts. However, Maraschin-Silva and Aqüila [21], investigating the allelopathic potential of this species, only infer about the presence of tannins and saponins in aqueous leaf extract.Arantes [17] analyzed the ethanol extract of leaves collected in Southern Brazil by HPLC and reported the presence of the flavonoids quercetin (1), rutin (2), and kaempferol (4) and aromatic acids gallic acid (5), chlorogenic acid (6), and caffeic acid (7) (Figure 1).Tanaka et al. [22] isolated from the methanol extract of leaves ofL. divaricata, collected in Maringá, Paraná State, Southern Brazil, a new triterpene (basic skeleton of ursene): 3β-p-hydroxybenzoyloxytormentic acid [3β-(p-hydroxybenzoyloxy)-2α-hydroxyurs-12-en-28-oic acid] (8), a mixture of tormentic acid ester glucoside (9), tormentic acid (10), and the maslinic acid (olean-12-ene-2α,3β-diol) (11) (basic skeleton of oleanane) (Figure 1).Tanaka et al. [10], in continuation of previous work, isolated from stem bark and leaves, besides the mentioned triterpenes, the steroid glucopyranosylsitosterol (12), (−)-epicatechin (13), a flavonoid which belongs to a class of flavan-3-ol, and vitexin (3), as already indicated in the same species isolated in previous work [18] (Figure 1).The presence of flavonoids such as vitexin (3) [23, 24] and triterpenes as maslinic acid (11) [25, 26] may be associated with the popular indication of its anti-inflammatory action [27].Besides polyphenols (flavonoids, catechins, anthocyanins, and tannins) the phytochemical screening of the hydroalcoholic extract of bark ofL. divaricata collected in Leme, São Paulo State, Southeastern Brazil, identified the presence of saponins, triterpenes, steroids, and anthracenes [28]. Walker et al. [29], in histochemical and morphoanatomical study of leaves ofL. divaricata Mart., reported about the presence of mucilage and calcium oxalate in the form of a prism and drusen in idioblasts.Luehea ochrophylla. Two papers that focus on the phytochemical analysis of this species are listed in this review. The phytochemical study of skins ofL. ochrophylla collected in Grão Mogol, Minas Gerais State, Southeastern Brazil, resulted in the isolation of hydrocarbons, aliphatic esters of steroid β-sitosterol (14), and pentacyclic triterpenes friedelin (15) and β-friedelinol (16) [30] (Figure 2).Figure 2
Phytocompounds isolated fromLuehea ochrophylla.


## 2.1.2.Luehea grandiflora

The works of da Silva et al. [30] that indicate polyphenols in the cortex ofL. grandiflora and Rosa et al. [31, 32] were the only works with focus on the phytochemistry of this species. Rosa et al. [31, 32] working with crude ethanol extract of leaves collected in Goiás State, Brazil, observed, from fractionation by column chromatography isolation, in chloroform fraction, the lupeol triterpene (16) and the mixture of steroids β-sitosterol (14), stigmasterol (17), and campesterol (18) (Figure 3).Figure 3
Phytocompounds isolated fromLuehea grandiflora.


## 2.1.3.Luehea candida

The anticancer potential ofL. candida extracts was evaluated against many cell lines and lupeol (16), betulin (19), (−)-epicatechin (13), vitexin (3), and a lignan liriodendrin (20) were isolated from the active fractions [8] (Figure 4).Figure 4
Phytocompounds isolated fromLuehea candida.Saénz and Nassar [33] established the presence, in small quantities, of alkaloids in this species. Alkaloids were also pointed out in qualitative studies onLuehea seemannii collected in Eastern Nicaragua [34] and leaves and bark in this species collected in Villa Neily, Costa Rica [35, 36].


## 2.1.4.Luehea paniculata

Barbosa and Reed [35, 36] analyzing ethanol extracts ofL. grandiflora andL. paniculata collected in Goiania, Brazil, inferred about the presence of tannins and flavonoids in leaves and bark of both species. The existence of rutin (2) inL. paniculata was identified by means of thin-layer chromatography.Calixto Júnior et al. [37] observed by HPLC-DAD fingerprinting inL. paniculata ethanolic extracts (leaves and sapwood) the presence of the gallic acid (5), chlorogenic acid (6), and rosmarinic acid (21) and flavonoids rutin (2), vitexin (3), and luteolin (22) (Figure 5).Figure 5
Phytocompounds isolated fromLuehea paniculata.Alves et al. [38] reported the isolation of triterpenes maslinic acid (11) from the chloroform fraction and oleanolic acid (23) and lupenone (24) from the ethyl acetate fraction, as well as evaluating the antiproliferative activity of the crude extract fractions of ethyl acetate and hydromethanol obtained from the leaves ofL. paniculata collected in the Cerrado, Central Brazil (Figure 5).


## 2.1.5.Luehea candicans

Silva [39], in a study that involved extraction, isolation, and identification of the chemical constituents of the branches and leaves ofL. candicans in the Puerto Rico region, Paraná State, Southern Brazil, got the isolation, the crude methanol extract of the stems of two triterpenes belonging to the class of lupins, the lup-20(29)-en-3β-ol (lupeol) (16) and the betulin—3β,28-diidroxilup-20(29)-ene (19). The works of Silva [39] and Da Silva et al. [8] were the only ones found on chemical research in this species.The presence of vitexin in the leaves ofL. candicans,L. divaricata, andL. paniculata species can suggest the hypothesis that this flavone may be a possible taxonomic marker of the genusLuehea.


## 2.2. Ethnopharmacological and Pharmacological Aspects

The popular knowledge about the medicinal uses of horse-whips is reported by several authors [40]; however, there are few studies available on the pharmacological potential of the species [12]. The highest number of citations on ethnopharmacological information is assigned to the speciesL. divaricata; however, some other species appear in other surveys.Luehea seemannii is popularly used against bites and stings (snake, scorpion, and insects) and also as an astringent in Eastern Nicaragua [34].In a survey conducted about ethnopharmacology of medicinal plants of the Pantanal (Mato Grosso State, Brazil),L. divaricata is among the species with the highest relative importance value (1.50) between values ranging from 0.17 to 1.87, among the 261 species mentioned in the work [27]. The authors report citations, by respondents, about the use ofL. divaricata in the treatment of lung and upper respiratory disease. However, they stress that no scientific evidence exist on the activity of the species in the regulation of cough, while its antibiotic properties also vary. Bessa et al. [41] reported a quote from “açoita-cavalo” as being of use to the treatment of ulcer in rural community of Green Vale Settlement, Tocantins State, Central Brazil.Several other reports of uses of this species were found: treatment of rheumatism; arthritis; dysentery; internal cleaning of wounds and ulcers; depurative; sore throat; painkiller for toothache; astringent; evils of the bladder; sleep balance; melena (intestinal colic followed by diarrhea with painful bowel movements and presence of blood in the stool); leucorrhea; gonorrhea; bleeding; cough; laryngitis; bronchitis; deworming; gastritis; poor digestion; diarrhea; and cancer and tumors [9, 42–44].Degen et al. [45] reported medicinal plants traded by their common names and cited “Francisco Álvarez” asL. divaricata being employed against diabetes in Argentina and in Paraguay, where the same common name matchesthe Banara arguta Brig. (Flacourtiaceae). The term “Francisco Álvarez” as reference to “açoita-cavalos” in Paraguay is also cited by Carvalho [46], which emphasizes the use of other common names abroad: azota caballo and árbol de San Francisco, Argentina; Francisco Álvarez, Uruguay; and ka’a oveti, Paraguay. In various regions of Brazil the author highlights açoita: açoita-cavalo-do-miúdo, açoita-cavalo-branco, ivantingui, and vatinga in the São Paulo State; açoita-cavalo in Paraná State; in the states of Rio de Janeiro and São Paulo, biatingui, erviteira-do-campo, estibeiro, and estriveira; in Bahia State and São Paulo State, guaxima-do-campo, ibatingui, and ivatingui; in Minas Gerais State, ivitinga; in Bahia State, ivitingui, luitingui, mutamba, and pau-de-canga; in Santa Catarina State, salta-cavalo; in Paraná State and São Paulo State, soita and soita-cavalo; and, in Paraná State, ubatinga.In folk medicine the “açoita-cavalo” is used in cases of dysentery, bleeding, arthritis, leucorrhoea, rheumatism, and tumors [41]. In ethnodirected work at Quilombo Sangrador, Maranhão State, Northeastern Brazil, the “açoita-cavalo” was one of the species with more valuable importance, with indications for the treatment of diseases of the genitourinary system [47]. Basualdo and Soria [48] includedL. divaricata Mart. in the list of medicinal plants of Paraguay used in fighting respiratory infections.The leaves ofLuehea are marketed as herbal against leucorrhoea, rheumatism, gonorrhea, and tumors; infusion of the flowers is used against bronchitis and the root is depurative [10]. Rai [11] reports that the aerial parts of “açoita-cavalo” (L. divaricata Martius et Zuccarini) are used in traditional medicines for skin wounds, grain cleaning, and vaginal washings. Maffei [49] in work on medicinal plants of Uruguay and Chiriani [50] in study about phytotherapy in Argentina inform that the barks are used as antipyretic and antianemic agents, in addition to antidiarrheal, astringent, and antitumor. Quotes on the leaves as herbal against rheumatism, dysentery, gonorrhea, soothing, and antispasmodic are observed in several works [10, 49, 51–53]. The root is pointed out as anti-inflammatory and cleansing by Alice et al. [54], and the flowers are cited, along with bark and leaves, with diuretic, antiarthritic, antileucorrhoea, and wound healing of the skin and vaginal washings [22, 40, 49, 55, 56]. The bath of the stem bark was quoted in ethnobotanical survey in the Upper Rio Grande, Minas Gerais State, Southeastern Brazil, [57] as being of use for dysentery, as antirheumatic and antihemorrhagic agent.According to Alice et al. [58], the bark of theL. divaricata is also used to combat the fever and is also suitable for gastrointestinal and liver disorders. The leaves are used as anti-inflammatory and employed in disorders of the respiratory tract and bronchitis. In popular phytotherapy the cortex of stems ofL. divaricata is used in decoctions and infusions by tonic and antidiarrheal characteristics [59]. The resin of the fruit is applied as antiodontalgic; 1% flowers infusion is used as sedative and the infusion of the leaves as anti-inflammatory [60, 61].


## 2.3. Microbiological Activities

Five studies have reported effects against microorganisms in this species. The antifungal activity ofL. divaricata was evaluated by Zacchino et al. [62]. As a result, the authors pointed out the moderate action of dichloromethane extract in inhibiting the growth of hyphae of some species of dermatophytes; however, this inhibitory effect is not observed for other fungal species [10, 40]. However, the extract ofL. divaricata showed strong inhibition of the growth ofStaphylococcus aureus,Staphylococcus epidermidis,Klebsiella pneumonia, andEscherichia coli in one study [40] but showed only moderate inhibition in another study and another collection site [10].Montovani et al. [63] also investigated antimicrobial action in extract ofL. divaricata. Collected in São Pedro do Iguaçu, Paraná State, Southern Brazil, the authors obtained extracts of leaves from three solvents (hexane, ethyl acetate, and ethanol) and diagnosed positive effects in inhibitingS. aureus,Bacillus cereus,E. coli, andSalmonella typhi. However, for the fungusAspergillus niger, they obtained negative result; Coelho De Souza et al. [64], as a result of ethnopharmacological study, evaluated the antimicrobial potential of some plants in common use in the Rio Grande do Sul State (Southern Brazil) using the agar diffusion method; the authors found that the methanol extract ofL. divaricata showed activity against theMicrococcus luteus bacteria. The extract showed no activity against other bacteria such asS. aureus,S. epidermidis, andE. coli, even against yeastCandida albicans.Marques et al. [65] reported the biological activities study ofL. paniculata of the Brazilian Cerrado, which showed inhibitory effect of ethanol extract ofL. paniculata leaves onS. aureus bacteria.Calixto Júnior et al. [37] pointed out as irrelevant the activity ofL. paniculata leaf ethanolic extract (LEELP) tested against six strains ofCandida (MIC ≥ 1024). This work is the first recorded piece of research on the potentialization of Fluconazole using extracts ofL. paniculata against three strains ofCandida, in particular,C. tropicalis andC. albicans, and there was, therefore, a synergism when the extracts were combined with Fluconazole.


## 2.4. Anti-Inflammation and Toxicity

Lopes [18] investigated the anti-inflammatory action ofL. divaricata. From an experiment using the aqueous extract obtained from the dried leaves of the plant, the authors used the test of rat paw edema induced by carrageenan. The extract was administered intraperitoneally at doses of 100 and 150 mg/kg and orally, using a stomach tube at a dose of 300 mg/kg. According to the authors, the dose of 150 mg/kg administered intraperitoneally obtained the peak reduction of the edema. Siqueira [28] claims to have the hydroalcoholic extract ofL. divaricata that reduced the rate of ulcerative lesions produced by indomethacin and ethanol. The author indicates that the mechanism of action antiulcerogenic is partly related to the activity of sulfhydryl radicals and by the precipitation of proteins produced by the presence of polyphenols present in the plant.Bianchi [66] conducted studies of acute and subacute toxicity with extracts of this species. In both tests Swiss mice, males, were used. The alcoholic extract administered intraperitoneally at doses of 250 mg/kg and 500 mg/kg triggered diarrhea and bristling fur, within 72 hours, that were observed in the acute toxicity test. 50% of deaths of the animals were seen using a dose of 500 mg/kg for 48 hours. In the subacute toxicity test, the authors administered a dose of 25 mg/kg of aqueous and alcoholic extract by means intraperitoneally, once a week for 8 days. The aqueous extract triggered the death of an animal after the 8th dose, unlike the alcoholic extract that at this dose did not cause any deaths [67]. Vargas et al. [20] showed genotoxic (mutagenic) activity in aqueous leaf extract ofL. divaricata in the Ames test (Salmonella/microsome) with microsomal activation. This fact can be explained by the presence of tannins and flavones in the leaves of the species that present patterns of hydroxylation, which could possibly cause damage to the DNA structure [54].The phytotoxic activity ofL. divaricata was shown clearly by Souza et al. [68]. The authors point out the test with aqueous extract of leaves collected in Pelotas, Southern Brazil, which showed an inhibitory effect on the germination ofLactuca sativa. Nevertheless,L. divaricata extracts demonstratein vivo lack of toxicity or mutagenicity [69, 70].


## 2.5. Antioxidant Potential

Good antioxidant activity and analgesic properties in leaves ofL. divaricata were pointed out by Müller [40]. By DPPH method, the author observed the crude extract and ethyl acetate and butanol fractions with similar antioxidant with the quercetin. Arantes [17] showed antioxidant activity at low concentrations of ethanol extract of the leaves of this species, observed by reducing basal levels of lipid peroxidation by the extract and its protective effect against lipid peroxidation and decreased cell viability induced by nitroprusside sodium in rat brainin vitro.Antioxidant effect of the ethanol extract ofLuehea paniculata roots was evaluated by the method of scavenging free radical DPPH [37]. This extract demonstrated an antioxidant potential almost similar to the value associated with the flavonoid quercetin (EC50 = 0.85 and 0.01 mg/mL, resp.). Calixto Júnior et al. [37] showed good results in antioxidant test of leaf extract and bark with IC50 values: 0.32 and 0.24 mg/mL, respectively.


## 2.6. Antiproliferative Activity

Alves et al. [38] analyzed the antiproliferative activity of the crude extract and ethyl acetate and hydromethanol fractions ofL. paniculata leaves. The evaluation was performed at concentrations from 0.25 to 250 mg/mL against three human tumor cell lines, breast (MCF-7), lung (NCI-H460), and glioma (U251), by the colorimetric method with sulforhodamine B. The results showed moderate antiproliferative potential for those fractions.The crude methanolic extracts of the branches and leaves ofLuehea candicans were evaluated using the following cancer cell lines: MCF-7 (breast), NCI-ADR (breast expressing the multidrug resistance phenotype), NCI-460 (lung), UACC-62 (melanoma), 786-0 (kidney), OVCAR (ovarian), PCO-3 (prostate), HT-29 (colon), and K-562 (leukaemia). The crude methanolic extracts from the branches (B) and leaves (L) were able to inhibit the growth of the K-562 and 786-0 cell lines in a dose-dependent manner, with GI50 values of 8.1 and 5.4 µg /mL, respectively. The hexane (L1), chloroform (L2), and methanol (L4) fractions derived from extract L showed a high selectivity and pronounced cytostatic activity against 786-0 (G
I
50
~40 µg /mL). A significant amount of lupeol was isolated from fraction L2. The chloroform (B2) and methanol (B3) fractions derived from extract (B) exhibited less selectivity, showing the highest cytostatic activity against K-562, NCI-ADR, OVCAR, MCF-7, and NCI-460 cells, with GI50 values between 27 and 40 µg/mL [8].Silva [39] studiedL. candicans in the region of Puerto Rico, Paraná State, Southern Brazil, and assessed, by biological tests, the antibacterial, antifungal, and antiproliferative crude extracts, fractions, and isolated compounds. None of the extracts, however, showed significant antibacterial activity; however, the crude extract of the leaves showed antifungal activity againstCandida krusei strain with a fungicide at a concentration of 125 mg/mL (CMF) and fungistatic concentration of 62.5 mg/mL (CIM). The crude extract ofL. candicans still showed antiproliferative activity with cytostatic effect of 85% (25 μg/mL) and cytocidal effect by 25%, the highest concentration used for cell kidney cancer. The hexane, CHCl3, and MeOH fractions demonstrated a cytostatic effect of 100% against kidney cancer cells. The crude extract of the stems showed cytostatic and antiproliferative activity with little selectivity among the studied strains (25% cell death at the highest concentration) effects, since the crude extract ofL. candicans and hexane, CHCl3 and MeOH fractions were selective for cells of kidney cancer exhibiting antiproliferative activity with cytostatic effect (growth inhibition) by 85% at a concentration of 25 mg/mL for the crude extract and 100% (250 μg/mL) for fractions. The antifungal activity of triterpenes, steroids, and flavonoids isolated was also tested, but without any substances presenting activity.


## 3. Conclusion

Studies confirm in part the medicinal uses of plants named as “açoita-cavalo” species. Some pharmacological activities not assigned to the species of the genusLuehea by populations were observed in laboratory experiments.28 papers focusing on phytochemical studies with species of the genus were observed. The most studied in this regard areL. divaricata,L. paniculata, andL. candicans. The chemical composition of the leaf and stem is similar in different species, leading to the assertion that similar actions relate to common components in different representatives of the genus. Several secondary metabolites were found as triterpenes, steroids, flavonoids, lignans, organic acids, and flavone vitexin.The vitexin, a C-glycosylated flavone, isolated from three different species ofLuehea is cited as a possible marker of taxonomic genus and to prove this hypothesis further chemical research studies are required to find it in other species. The flavone vitexin and triterpene maslinic acid may be associated with anti-inflammatory action, which is considered the main popular indication for the genus.



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*Source: 1368971-2016-10-13.xml*