# Nuclear Medicine in Diagnosis of Prosthetic Valve Endocarditis: An Update

Authors: Maria Musso; Nicola Petrosillo
Journal: BioMed Research International
 (2015)

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## Abstract

Over the past decades cardiovascular disease management has been substantially improved by the increasing introduction of medical devices as prosthetic valves. The yearly rate of infective endocarditis (IE) in patient with a prosthetic valve is approximately 3 cases per 1,000 patients. The fatality rate of prosthetic valve endocarditis (PVE) remains stable over the years, in part due to the aging of the population. The diagnostic value of echocardiography in diagnosis is operator-dependent and its sensitivity can decrease in presence of intracardiac devices and valvular prosthesis. The modified Duke criteria are considered the gold standard for diagnosing IE; their sensibility is 80%, but in clinical practice their diagnostic accuracy in PVE is lower, resulting inconclusively in nearly 30% of cases. In the last years, these new imaging modalities have gained an increasing attention because they make it possible to diagnose an IE earlier than the structural alterations occurring. Several studies have been conducted in order to assess the diagnostic accuracy of various nuclear medicine techniques in diagnosis of PVE. We performed a review of the literature to assess the available evidence on the role of nuclear medicine techniques in the diagnosis of PVE.


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## Body

## 1. Introduction

Endovascular infections, including infective endocarditis (IE) and prosthetic vascular infections, are uncommon pathologies associated with a poor prognosis. Over the past decades cardiovascular disease management has been substantially improved by the increasing introduction of medical devices as mechanical or biologic heart valves, pacemakers, defibrillators, coronary artery stents, and artificial arteries.The patients’ age has increased over the last years, leading to an increase in the number of cardiovascular surgical interventions and also in the infective risk of patients. Between 1990 and 1999 incidence of prosthetic valve endocarditis (PVE) has increased 50% [1, 2]; patients with heart valve prosthesis are exposed to a yearly rate of approximately 3 cases of IE per 1,000 patients [3].These changes in the epidemiology also explain why, despite important improvements in IE management, the fatality rate of PVE has not decreased in the last forty years, with in-hospital fatality rate of about 20% [4].PVE are mostly healthcare associated and are distinct in early onset (EO) and late onset (LO), according to the time spent from the intervention to the onset of symptoms; usually a cutoff of one year is adopted [2]. Rates of infection in PVE range from 1–6% to 15%, with higher rates in revision surgery [5].PVE diagnosis may still be problematic. Imaging investigations traditionally used for diagnosis of PVE are transthoracic echocardiography (TTE) and transesophageal echocardiography (TEE), with a sensitivity of 70% and 90%, respectively [3]; however, the diagnostic value of echocardiography in IE diagnosis is operator-dependent and its sensitivity can decrease in presence of intracardiac devices, valvular prosthesis, severe preexisting lesions, and very small or no vegetation [4].Over the past years nuclear medicine procedures gave an important contribution to the diagnostic assessment of endovascular infections, through the evaluation of the metabolic activity of the prosthetic material presenting morphological alterations or before development of structural changes.We performed a review of the literature to summarize the available evidence on the role of nuclear medicine techniques in the diagnosis of PVE.


## 2. Methods

Published papers from January 1980 to June 2014, represented by clinical studies, reviews, and case reports reporting data about use of nuclear medicine techniques to investigate prosthetic valve infections, were found through computerized literature searches using MEDLINE (National Library of Medicine, Bethesda, MD) and by reviewing the references of retrieved articles. Index search terms included the Medical Subjects Heading “nuclear medicine,” “prosthetic,” “valve,” and “endocarditis.” Only articles written in English, German, Spanish, and Italian were included.Infective endocarditis in patients with an implanted cardiac device and vascular graft was out of the scope of this paper.


## 3. Results and Discussion

### 3.1. Prosthetic Valve Endocarditis Diagnosis

#### 3.1.1. Current Practice in the Management of Endocarditis

The epidemiological characteristics of patients with IE differ between developed and developing countries; in the latter case the patient is younger and is affected by predisposing diseases, such as congenital heart disease or rheumatic fever. In developed countries medical progress has caused an increase in the age of patients with endocarditis and in the incidence of health care associated endocarditis, including PVE [4].Infection of the cardiac prosthetic valve can occur by contamination at the time of its placement or as a result of hematogenous dissemination or by contiguous infection [2].Staphylococcus aureus is the most common cause of PVE, followed by coagulase-negative staphylococci;Streptococcus viridans group,Enterococcus spp., and yeasts are more common in LO than in EO PVE. Culture-negative endocarditis represents approximately 13% of PVE, with reported rates of 31% [4] and they are more frequent in LO than in EO PVE. Chronic haemodialysis, diabetes, and intravascular devices, including cardiac prosthetic valve, are all predisposing factors toS. aureus endocarditis [24].From a clinical point of view, the presentation of PVE does not substantially differ from the other forms of IE. Indeed, its nonspecific clinical presentation makes IE a cause of fever of unknown origin, with a high clinical suspicion needed to make diagnosis.The modified Duke criteria are considered the gold standard for diagnosing IE [6] (Tables 1, 2, and 3). In epidemiological studies the sensitivity of Duke criteria is 80%, but in clinical practice their diagnostic accuracy is lower, especially in PVE; prosthetic material in fact compromises the sensitivity of echocardiography that results inconclusively in nearly 30% of cases [4].Table 1
Modified Duke criteria for the diagnosis of prosthetic valve endocarditis (PVE) [6].
Definite PVE
Clinical criteria
(1) 2 major criteria
Or
(2) 1 major criterion and 3 minor criteria
Or
(3) 5 minor criteria
Pathological criteria
(1) Microorganisms demonstrated by culture or histological examination of a vegetation, a vegetation that has embolized
Or
(2) An intracardiac abscess specimen or pathological lesions; vegetation or intracardiac abscess confirmed by histological examination showing active endocarditis
Possible PVE
(1) 1 major criterion and 1 minor criterion
Or
(2) 3 minor criteria
Rejected PVE
(1) Firm alternate diagnosis explaining evidence of infective endocarditis
Or
(2) Resolution of infective endocarditis syndrome with antibiotic therapy for ≤4 days
Or
(3) No pathological evidence of infective endocarditis at surgery or autopsy, with antibiotic therapy for ≤4 daysOr
(4) Does not meet criteria for possible infective endocarditis, as aboveTable 2
Major criteria according to Li et al. [6] and new major criteria according to Saby et al. [7] for the diagnosis of prosthetic valve endocarditis (PVE).
Li et al. [6]
Saby et al. [7]
Blood culture positive for infective endocarditis(i) Typical microorganisms consistent with infective endocarditis from 2 separate blood cultures.Viridans streptococci, Streptococcus bovis, HACEK group, Staphylococcus aureus,or community-acquired enterococci, in the absence of a primary focus (ii) Microorganisms consistent with infective endocarditis from persistently positive blood cultures, defined as follows.≥2 positive cultures of blood samples drawn >12 hapart or all of 3 or a majority of ≥4 separate cultures of blood (with first and last sample drawn at least1 hapart) (iii) Single positive blood culture for Coxiella burnetii or antiphase I IgG antibody titer >1 : 800Evidence of endocardial involvement (i) Echocardiogram (TTE and/or TEE) positive for infective endocarditis defined as follows.Oscillating intracardiac mass on valve or supporting structures, in the path of regurgitant jets, or on implanted material in the absence of an alternative anatomic explanation or abscess or new partial dehiscence of prosthetic valve (ii) New valvular regurgitation (worsening or changing of preexisting murmur not sufficient).
Duke major criteriaandpositive 18F-FDG PET/CT: abnormal FDG uptake at the site of prosthetic valve
18F-FDG: 18F-fluorodeoxyglucose; HACEK:Haemophilus species, Actinobacillus actinomycetemcomitans, Cardiobacterium hominis, Eikenella corrodens, and Kingella species; IgG: immunoglobulin G; PET/CT: positron emission tomography/computed tomography; TEE: transesophageal echocardiography; TTE: transthoracic echocardiography.Table 3
Minor criteria for the diagnosis of prosthetic valve endocarditis (PVE) [6].
(i) Predisposition, predisposing heart condition, or injection drug use(ii) Fever, temperature ≥38°C(iii) Vascular phenomena: major arterial emboli, septic pulmonary infarcts, mycotic aneurysm, intracranial hemorrhage, and conjunctival hemorrhages(iv) Janeway lesions(v) Immunological phenomena: glomerulonephritis, Osler nodes, Roth spots, and rheumatoid factor(vi) Microbiological evidence: positive blood culture but does not meet a major criterion as noted above or serological evidence of active infection with organism consistent with infective endocarditisPVE represents up to 30% of IE in developed countries, and the in-hospital mortality in patients with PVE can reach 47%, especially inS. aureus bacteremia; these data confirm that it is important to increase the ability to early diagnose this clinical condition [8].


#### 3.1.2. The Contribution of Nuclear Medicine in the Diagnosis of PVE

Several nuclear medicine techniques, characterized by different kind of tracers and technologies, have been implemented in order to offer precious information about the metabolic activity of infected tissue. In the last years, these new imaging modalities have gained an increasing attention because they make it possible to diagnose an IE earlier than the structural alterations occurring.Since the eighties to nowadays several papers have reported the experiences of nuclear medicine (NM) specialists in utilization of NM imaging modalities for PVE diagnosis. Our research provided 29 articles; seven were excluded because they were inadequate to our purpose.Table4 summarizes all techniques mentioned in papers reviewed [7–23]; we did not include 6 commentary articles to the prospective study of Saby et al. that we have already included in Table 4 [25–30].Table 4
Nuclear medicine (NM) in the diagnosis of prosthetic valve endocarditis (PVE): review of the literature.
NM imaging modalities
Number of articles/number of patients
Type of paper/reference/year
18F-FDG-PET/TC
1/not valuable
Review/[8]/2013
1/72
Prospective study/[7]/2013
2/2
Case report/[9, 10]/2013, 2009
1/6
Case series/[11]/2014
CT angiography and18F-FDG-PET
1/7
Case series/[12]/2013
111Indium labeled leukocytes scintigraphy
2/2
Case report/[13, 14]/1990, 1988
1/2
Case series/[15]/1989
67Gallium citrate-SPECT and SPECT/TC
4/4
Case report/[16–19]/2005, 2009, 2002, 1991
99mTechnetium-HMPAO-WBC-SPECT/TC
1/131
Prospective study/[20]/2012
1/42
Prospective study/[21]/2013
99mTechnetium anti-G mAb-SPECT
1/1
Case report/[22]/1991
1/38
Prospective study/[23]/1995
FDG: fluorodeoxyglucose; PET/TC: positron emission tomography/computed tomography; CT: computed tomography; SPECT/TC: single photon emission computed tomography/computed tomography; HMPAO: hexamethylpropylene amine oxime; WBC: white blood cells; anti-G mAb: anti-human granulocytes antibodies.Ten articles reported experiences regarding labeled leukocytes scintigraphy in PVE diagnosis [13–23]; in some of them this technique was associated with single photon emission computed tomography (SPECT) acquisition of images [16–19], because SPECT can provide accurate morphologic details.Six papers regard18F-fluorodeoxyglucose positron emission tomography in association with computed tomography (18F-FDG PET/CT) or with computed tomography angiography (CTA) in the diagnostic assessment of PVE or perivalvular infective complications in patient affected by PVE [12–21].(1)
18
F-FDG PET/CT. 18F-FDG PET/CT combines the assessment of metabolic activity of tissues (namely, the glycolytic activity) with the spatial resolution of CT.A review published in 2013 has extensively collected the available data, mainly from case reports and case series, on the efficacy and usefulness of this technique and has stressed that there is currently no evidence from case-control studies comparing PET/CT with other diagnostic tools currently available [8].To date there has been only one published prospective study that aims to investigate the diagnostic power of this technique [7]. Seventy-two patients with suspected PVE, initially assessed by using the modified Duke criteria, underwent PET/CT. Although the diagnosis of endocarditis was not always confirmed by histological examination of the prosthetic valve, the authors point out that all those cases of endocarditis undergoing surgery and confirmed by pathologists were identified by 18F-FDG PET/CT [7].Sensitivity and specificity reported in this study (73% and 80%, resp.) did not differ greatly from the values found in a recent study on the use of18F-FDG PET/CT for the diagnosis of endocarditis on pacemakers and implantable defibrillators where sensitivity and specificity were 63% and 86, respectively [31].However, this study provides interesting data on the possible inclusion of18F-FDG PET/CT among the major criteria of Duke, as already proposed by Millar et al. [7, 8] (Table 2). In fact, the sensitivity of these “new” modified Duke criteria rises up to 97%, which would increase the rate of the so-called “defined” endocarditis [7].New diagnostic algorithms have been proposed in order to ensure an early diagnosis of endocarditis in those cases in which the Duke criteria are inconclusive; this happens in case of culture-negative endocarditis or in those cases in which transthoracic echocardiography (TTE) and transesophageal echocardiography (TEE) do not visualize lesions of the valve [7, 8].In this regard, in a case report, Gouriet et al. describe the representative contribution of PET/CT to the diagnosis of PVE in case of “possible endocarditis,” according to the Duke criteria. In this study, PET/CT was performed in course of antibiotic therapy for a knee prostheses infection, after 3 TEEs repeated 7 days apart from each other because of a strong suspicion of IE. The new findings of mitral regurgitation and prolapse of one of the mitral cusps at the third TEE have led to the diagnosis of possible endocarditis; the PET TC subsequently performed made it possible to diagnose a definitive PVE [9].New imaging modalities, as computed tomography angiography (CTA), have been proposed as a supplement to PET, as Tanis et al. reported in their case series [12]. The authors describe 7 cases of PVE, where the integration of the two techniques allowed diagnosing PVE unidentified by TEE, and periannular complication, that is, mycotic aneurism of coronary arteries and myocardial abscesses [12].There are still some controversies about various issues related to the PET/CT methodology.First, the resolving power of this technique permits the detection of lesions of 5 mm and over. However, according to some authors, this is not a limit to the diagnostic power as even smaller lesions, if there is a sufficient uptake, can be displayed. In this regard, Bertagna and colleagues observed that the standard protocol that is used in most nuclear medicine centers is the oncological one that consists of imaging acquisition performed 1 hour after glucose injection [26]. Therefore, they suggest acquiring images even at 2-3 hours after the injection of glucose in order to detect uptake by monocytes and macrophages at the level of the lesion and to increase the sensitivity of the PET/CT methodology [26].Moreover, there is concern about the specificity of the method in the early period after prosthesis placement. A functional glucose uptake persists in fact from 1 month up to 1 year after surgery. This variability between individuals is influenced by various factors, such as age, clinical status, and overall cardiac condition of the patient. Some authors have also expressed concern about the lack of controls in the prospective study published by Saby et al. [7], because, in order to correctly interpret the values of uptake and the validity of the exam, it is necessary to compare with a normal pattern of glucose uptake, in the absence of infection [30].(2) Labeled Leukocytes Scintigraphy. 111Indium is one of the first radiopharmaceuticals used in detecting cardiovascular infections (Table 4) [12–14]. Articles collected [12–14] describe scintigraphy with 111Indium employed in diagnosing prosthetic aortic perivalvular abscesses that is a severe endocarditis complication undetectable with traditional radiological tools. This complication is common; it complicates PVE in approximately 50% of cases and needs an urgent surgical approach [20].111Indium seems more efficacious to detect perivalvular complications than valvular surface infections, but its time-consuming images’ acquisition limits its utilization.67Gallium citrate has been used more often than 111Indium as tracer for scintigraphy in cardiovascular infection field, and it has always been associated with SPECT views [16–19]. One of the articles regarding 67Gallium citrate reports its capability to detect endocarditis in absence of any morphological alteration at echocardiography [18].A retrospective study assessed the value of 99mTc-HMPAO- (hexamethylpropylene amine oxime-) WBC (white blood cells) scintigraphy including SPECT/CT acquisitions in a series of 131 consecutive patients with suspected IE, 68% of whom with prosthetic valves [20].99mTc-HMPAO-WBC scintigraphy results were correlated with transthoracic or transesophageal echocardiography, blood cultures, and the Duke criteria.Technetium99m-HMPAO-SPECT/CT was positive in all cases of IE in which at least one major Duke criterion was present; moreover, it was positive in IE cases, ascertained by microbiological or clinical diagnosis, with a negative echocardiography [20].Technetium
99
m labelled monoclonal anti-human granulocytes antibodies (Anti-G mAb) may be employed to label leukocytes in vivo to detect inflammation and infection, but literature regarding its contribution to PVE diagnosis is scarce. Our literature search provided two papers reporting, respectively, a case report and a small prospective case-control study [22, 23]. In this latter article sensitivity and specificity of scintigraphy, in combination with echocardiography, were 100% and 80%, respectively, but the clinical sample was limited, that is, 18 cases and 20 controls [23]. Currently there are no sufficient evidences to suggest this technique as a reliable tool in PVE diagnosis.


#### 3.1.3. The Contribution of Nuclear Medicine to the Diagnosis of Complications in IE, Including PVE

Complications of PVE are rare but worrying because they often require an urgent surgical intervention. They include septic embolism, perivalvular infection, myocardial abscesses, and prosthetic valve dehiscence.In this regard a retrospective study provided interesting data about the usefulness ofTechnetium99m-HMPAO-SPECT/CT in assessment of cardiac surgery indications in patients with a suspect PVE [21].With this technique the authors diagnosed extensive perivalvular infections and abscesses in patients with confirmed endocarditis and no perivalvular extension of the infection detected by TEE; surgery confirmed the presence of an abscess in those patients with positive scintigraphy who underwent surgical intervention, with a positive predictive value for the presence of an abscess of 100% [21].It is well known that nuclear medicine contributes to the diagnosis of septic metastatic foci in cases of systemic infections, as reported by Vos et al. in a prospective study regarding 115 patients affected by bacteremia [32].Embolization and/or metastatic infection occurs in more than half of patients with IE and most commonly involves the spleen, the kidney, the liver, and the iliac or mesenteric arteries [33].Embolic events have been reported in 22–43% of patients with IE, mainly in the first 2 weeks after initiation of antibiotic therapy. A misdiagnosed septic embolism can cause a subsequent seeding of the infection at the metastatic site, a persistent bacteremia, and colonization of newly placed prosthetic valve [34].Metastatic infection can occur with different form, for example, septic arthritis, spondylodiscitis, pericarditis, and abscess [33, 35]. Their presence influences the appropriate choice of antibiotic therapy and surgical management, the duration of followup, and the outcome of IE [34]. The contribution of 18F-FDG PET/CT to the early diagnosis of embolism and metastatic infection in patients with IE has been reported in recent prospective studies [34, 36]; peripheral embolism and/or metastatic infection were detected in 44% of IE episodes, 40% of which were PVE [34].Moreover, PET/CT detected unexpected extracardiac sites of infection, that is, asymptomatic cases, in 24%–28% of IE [34, 36].Finally, a retrospective study by Erba et al. on theTechnetium99m-HMPAO-SPECT/CT contribution to the assessment of suspected IE reported the usefulness of this technique for the identification of septic emboli, detected in 41% of patients, even in the absence of the typical echocardiographic predictors of systemic embolism [20].


## 3.1. Prosthetic Valve Endocarditis Diagnosis

### 3.1.1. Current Practice in the Management of Endocarditis

The epidemiological characteristics of patients with IE differ between developed and developing countries; in the latter case the patient is younger and is affected by predisposing diseases, such as congenital heart disease or rheumatic fever. In developed countries medical progress has caused an increase in the age of patients with endocarditis and in the incidence of health care associated endocarditis, including PVE [4].Infection of the cardiac prosthetic valve can occur by contamination at the time of its placement or as a result of hematogenous dissemination or by contiguous infection [2].Staphylococcus aureus is the most common cause of PVE, followed by coagulase-negative staphylococci;Streptococcus viridans group,Enterococcus spp., and yeasts are more common in LO than in EO PVE. Culture-negative endocarditis represents approximately 13% of PVE, with reported rates of 31% [4] and they are more frequent in LO than in EO PVE. Chronic haemodialysis, diabetes, and intravascular devices, including cardiac prosthetic valve, are all predisposing factors toS. aureus endocarditis [24].From a clinical point of view, the presentation of PVE does not substantially differ from the other forms of IE. Indeed, its nonspecific clinical presentation makes IE a cause of fever of unknown origin, with a high clinical suspicion needed to make diagnosis.The modified Duke criteria are considered the gold standard for diagnosing IE [6] (Tables 1, 2, and 3). In epidemiological studies the sensitivity of Duke criteria is 80%, but in clinical practice their diagnostic accuracy is lower, especially in PVE; prosthetic material in fact compromises the sensitivity of echocardiography that results inconclusively in nearly 30% of cases [4].Table 1
Modified Duke criteria for the diagnosis of prosthetic valve endocarditis (PVE) [6].
Definite PVE
Clinical criteria
(1) 2 major criteria
Or
(2) 1 major criterion and 3 minor criteria
Or
(3) 5 minor criteria
Pathological criteria
(1) Microorganisms demonstrated by culture or histological examination of a vegetation, a vegetation that has embolized
Or
(2) An intracardiac abscess specimen or pathological lesions; vegetation or intracardiac abscess confirmed by histological examination showing active endocarditis
Possible PVE
(1) 1 major criterion and 1 minor criterion
Or
(2) 3 minor criteria
Rejected PVE
(1) Firm alternate diagnosis explaining evidence of infective endocarditis
Or
(2) Resolution of infective endocarditis syndrome with antibiotic therapy for ≤4 days
Or
(3) No pathological evidence of infective endocarditis at surgery or autopsy, with antibiotic therapy for ≤4 daysOr
(4) Does not meet criteria for possible infective endocarditis, as aboveTable 2
Major criteria according to Li et al. [6] and new major criteria according to Saby et al. [7] for the diagnosis of prosthetic valve endocarditis (PVE).
Li et al. [6]
Saby et al. [7]
Blood culture positive for infective endocarditis(i) Typical microorganisms consistent with infective endocarditis from 2 separate blood cultures.Viridans streptococci, Streptococcus bovis, HACEK group, Staphylococcus aureus,or community-acquired enterococci, in the absence of a primary focus (ii) Microorganisms consistent with infective endocarditis from persistently positive blood cultures, defined as follows.≥2 positive cultures of blood samples drawn >12 hapart or all of 3 or a majority of ≥4 separate cultures of blood (with first and last sample drawn at least1 hapart) (iii) Single positive blood culture for Coxiella burnetii or antiphase I IgG antibody titer >1 : 800Evidence of endocardial involvement (i) Echocardiogram (TTE and/or TEE) positive for infective endocarditis defined as follows.Oscillating intracardiac mass on valve or supporting structures, in the path of regurgitant jets, or on implanted material in the absence of an alternative anatomic explanation or abscess or new partial dehiscence of prosthetic valve (ii) New valvular regurgitation (worsening or changing of preexisting murmur not sufficient).
Duke major criteriaandpositive 18F-FDG PET/CT: abnormal FDG uptake at the site of prosthetic valve
18F-FDG: 18F-fluorodeoxyglucose; HACEK:Haemophilus species, Actinobacillus actinomycetemcomitans, Cardiobacterium hominis, Eikenella corrodens, and Kingella species; IgG: immunoglobulin G; PET/CT: positron emission tomography/computed tomography; TEE: transesophageal echocardiography; TTE: transthoracic echocardiography.Table 3
Minor criteria for the diagnosis of prosthetic valve endocarditis (PVE) [6].
(i) Predisposition, predisposing heart condition, or injection drug use(ii) Fever, temperature ≥38°C(iii) Vascular phenomena: major arterial emboli, septic pulmonary infarcts, mycotic aneurysm, intracranial hemorrhage, and conjunctival hemorrhages(iv) Janeway lesions(v) Immunological phenomena: glomerulonephritis, Osler nodes, Roth spots, and rheumatoid factor(vi) Microbiological evidence: positive blood culture but does not meet a major criterion as noted above or serological evidence of active infection with organism consistent with infective endocarditisPVE represents up to 30% of IE in developed countries, and the in-hospital mortality in patients with PVE can reach 47%, especially inS. aureus bacteremia; these data confirm that it is important to increase the ability to early diagnose this clinical condition [8].


### 3.1.2. The Contribution of Nuclear Medicine in the Diagnosis of PVE

Several nuclear medicine techniques, characterized by different kind of tracers and technologies, have been implemented in order to offer precious information about the metabolic activity of infected tissue. In the last years, these new imaging modalities have gained an increasing attention because they make it possible to diagnose an IE earlier than the structural alterations occurring.Since the eighties to nowadays several papers have reported the experiences of nuclear medicine (NM) specialists in utilization of NM imaging modalities for PVE diagnosis. Our research provided 29 articles; seven were excluded because they were inadequate to our purpose.Table4 summarizes all techniques mentioned in papers reviewed [7–23]; we did not include 6 commentary articles to the prospective study of Saby et al. that we have already included in Table 4 [25–30].Table 4
Nuclear medicine (NM) in the diagnosis of prosthetic valve endocarditis (PVE): review of the literature.
NM imaging modalities
Number of articles/number of patients
Type of paper/reference/year
18F-FDG-PET/TC
1/not valuable
Review/[8]/2013
1/72
Prospective study/[7]/2013
2/2
Case report/[9, 10]/2013, 2009
1/6
Case series/[11]/2014
CT angiography and18F-FDG-PET
1/7
Case series/[12]/2013
111Indium labeled leukocytes scintigraphy
2/2
Case report/[13, 14]/1990, 1988
1/2
Case series/[15]/1989
67Gallium citrate-SPECT and SPECT/TC
4/4
Case report/[16–19]/2005, 2009, 2002, 1991
99mTechnetium-HMPAO-WBC-SPECT/TC
1/131
Prospective study/[20]/2012
1/42
Prospective study/[21]/2013
99mTechnetium anti-G mAb-SPECT
1/1
Case report/[22]/1991
1/38
Prospective study/[23]/1995
FDG: fluorodeoxyglucose; PET/TC: positron emission tomography/computed tomography; CT: computed tomography; SPECT/TC: single photon emission computed tomography/computed tomography; HMPAO: hexamethylpropylene amine oxime; WBC: white blood cells; anti-G mAb: anti-human granulocytes antibodies.Ten articles reported experiences regarding labeled leukocytes scintigraphy in PVE diagnosis [13–23]; in some of them this technique was associated with single photon emission computed tomography (SPECT) acquisition of images [16–19], because SPECT can provide accurate morphologic details.Six papers regard18F-fluorodeoxyglucose positron emission tomography in association with computed tomography (18F-FDG PET/CT) or with computed tomography angiography (CTA) in the diagnostic assessment of PVE or perivalvular infective complications in patient affected by PVE [12–21].(1)
18
F-FDG PET/CT. 18F-FDG PET/CT combines the assessment of metabolic activity of tissues (namely, the glycolytic activity) with the spatial resolution of CT.A review published in 2013 has extensively collected the available data, mainly from case reports and case series, on the efficacy and usefulness of this technique and has stressed that there is currently no evidence from case-control studies comparing PET/CT with other diagnostic tools currently available [8].To date there has been only one published prospective study that aims to investigate the diagnostic power of this technique [7]. Seventy-two patients with suspected PVE, initially assessed by using the modified Duke criteria, underwent PET/CT. Although the diagnosis of endocarditis was not always confirmed by histological examination of the prosthetic valve, the authors point out that all those cases of endocarditis undergoing surgery and confirmed by pathologists were identified by 18F-FDG PET/CT [7].Sensitivity and specificity reported in this study (73% and 80%, resp.) did not differ greatly from the values found in a recent study on the use of18F-FDG PET/CT for the diagnosis of endocarditis on pacemakers and implantable defibrillators where sensitivity and specificity were 63% and 86, respectively [31].However, this study provides interesting data on the possible inclusion of18F-FDG PET/CT among the major criteria of Duke, as already proposed by Millar et al. [7, 8] (Table 2). In fact, the sensitivity of these “new” modified Duke criteria rises up to 97%, which would increase the rate of the so-called “defined” endocarditis [7].New diagnostic algorithms have been proposed in order to ensure an early diagnosis of endocarditis in those cases in which the Duke criteria are inconclusive; this happens in case of culture-negative endocarditis or in those cases in which transthoracic echocardiography (TTE) and transesophageal echocardiography (TEE) do not visualize lesions of the valve [7, 8].In this regard, in a case report, Gouriet et al. describe the representative contribution of PET/CT to the diagnosis of PVE in case of “possible endocarditis,” according to the Duke criteria. In this study, PET/CT was performed in course of antibiotic therapy for a knee prostheses infection, after 3 TEEs repeated 7 days apart from each other because of a strong suspicion of IE. The new findings of mitral regurgitation and prolapse of one of the mitral cusps at the third TEE have led to the diagnosis of possible endocarditis; the PET TC subsequently performed made it possible to diagnose a definitive PVE [9].New imaging modalities, as computed tomography angiography (CTA), have been proposed as a supplement to PET, as Tanis et al. reported in their case series [12]. The authors describe 7 cases of PVE, where the integration of the two techniques allowed diagnosing PVE unidentified by TEE, and periannular complication, that is, mycotic aneurism of coronary arteries and myocardial abscesses [12].There are still some controversies about various issues related to the PET/CT methodology.First, the resolving power of this technique permits the detection of lesions of 5 mm and over. However, according to some authors, this is not a limit to the diagnostic power as even smaller lesions, if there is a sufficient uptake, can be displayed. In this regard, Bertagna and colleagues observed that the standard protocol that is used in most nuclear medicine centers is the oncological one that consists of imaging acquisition performed 1 hour after glucose injection [26]. Therefore, they suggest acquiring images even at 2-3 hours after the injection of glucose in order to detect uptake by monocytes and macrophages at the level of the lesion and to increase the sensitivity of the PET/CT methodology [26].Moreover, there is concern about the specificity of the method in the early period after prosthesis placement. A functional glucose uptake persists in fact from 1 month up to 1 year after surgery. This variability between individuals is influenced by various factors, such as age, clinical status, and overall cardiac condition of the patient. Some authors have also expressed concern about the lack of controls in the prospective study published by Saby et al. [7], because, in order to correctly interpret the values of uptake and the validity of the exam, it is necessary to compare with a normal pattern of glucose uptake, in the absence of infection [30].(2) Labeled Leukocytes Scintigraphy. 111Indium is one of the first radiopharmaceuticals used in detecting cardiovascular infections (Table 4) [12–14]. Articles collected [12–14] describe scintigraphy with 111Indium employed in diagnosing prosthetic aortic perivalvular abscesses that is a severe endocarditis complication undetectable with traditional radiological tools. This complication is common; it complicates PVE in approximately 50% of cases and needs an urgent surgical approach [20].111Indium seems more efficacious to detect perivalvular complications than valvular surface infections, but its time-consuming images’ acquisition limits its utilization.67Gallium citrate has been used more often than 111Indium as tracer for scintigraphy in cardiovascular infection field, and it has always been associated with SPECT views [16–19]. One of the articles regarding 67Gallium citrate reports its capability to detect endocarditis in absence of any morphological alteration at echocardiography [18].A retrospective study assessed the value of 99mTc-HMPAO- (hexamethylpropylene amine oxime-) WBC (white blood cells) scintigraphy including SPECT/CT acquisitions in a series of 131 consecutive patients with suspected IE, 68% of whom with prosthetic valves [20].99mTc-HMPAO-WBC scintigraphy results were correlated with transthoracic or transesophageal echocardiography, blood cultures, and the Duke criteria.Technetium99m-HMPAO-SPECT/CT was positive in all cases of IE in which at least one major Duke criterion was present; moreover, it was positive in IE cases, ascertained by microbiological or clinical diagnosis, with a negative echocardiography [20].Technetium
99
m labelled monoclonal anti-human granulocytes antibodies (Anti-G mAb) may be employed to label leukocytes in vivo to detect inflammation and infection, but literature regarding its contribution to PVE diagnosis is scarce. Our literature search provided two papers reporting, respectively, a case report and a small prospective case-control study [22, 23]. In this latter article sensitivity and specificity of scintigraphy, in combination with echocardiography, were 100% and 80%, respectively, but the clinical sample was limited, that is, 18 cases and 20 controls [23]. Currently there are no sufficient evidences to suggest this technique as a reliable tool in PVE diagnosis.


### 3.1.3. The Contribution of Nuclear Medicine to the Diagnosis of Complications in IE, Including PVE

Complications of PVE are rare but worrying because they often require an urgent surgical intervention. They include septic embolism, perivalvular infection, myocardial abscesses, and prosthetic valve dehiscence.In this regard a retrospective study provided interesting data about the usefulness ofTechnetium99m-HMPAO-SPECT/CT in assessment of cardiac surgery indications in patients with a suspect PVE [21].With this technique the authors diagnosed extensive perivalvular infections and abscesses in patients with confirmed endocarditis and no perivalvular extension of the infection detected by TEE; surgery confirmed the presence of an abscess in those patients with positive scintigraphy who underwent surgical intervention, with a positive predictive value for the presence of an abscess of 100% [21].It is well known that nuclear medicine contributes to the diagnosis of septic metastatic foci in cases of systemic infections, as reported by Vos et al. in a prospective study regarding 115 patients affected by bacteremia [32].Embolization and/or metastatic infection occurs in more than half of patients with IE and most commonly involves the spleen, the kidney, the liver, and the iliac or mesenteric arteries [33].Embolic events have been reported in 22–43% of patients with IE, mainly in the first 2 weeks after initiation of antibiotic therapy. A misdiagnosed septic embolism can cause a subsequent seeding of the infection at the metastatic site, a persistent bacteremia, and colonization of newly placed prosthetic valve [34].Metastatic infection can occur with different form, for example, septic arthritis, spondylodiscitis, pericarditis, and abscess [33, 35]. Their presence influences the appropriate choice of antibiotic therapy and surgical management, the duration of followup, and the outcome of IE [34]. The contribution of 18F-FDG PET/CT to the early diagnosis of embolism and metastatic infection in patients with IE has been reported in recent prospective studies [34, 36]; peripheral embolism and/or metastatic infection were detected in 44% of IE episodes, 40% of which were PVE [34].Moreover, PET/CT detected unexpected extracardiac sites of infection, that is, asymptomatic cases, in 24%–28% of IE [34, 36].Finally, a retrospective study by Erba et al. on theTechnetium99m-HMPAO-SPECT/CT contribution to the assessment of suspected IE reported the usefulness of this technique for the identification of septic emboli, detected in 41% of patients, even in the absence of the typical echocardiographic predictors of systemic embolism [20].


## 3.1.1. Current Practice in the Management of Endocarditis

The epidemiological characteristics of patients with IE differ between developed and developing countries; in the latter case the patient is younger and is affected by predisposing diseases, such as congenital heart disease or rheumatic fever. In developed countries medical progress has caused an increase in the age of patients with endocarditis and in the incidence of health care associated endocarditis, including PVE [4].Infection of the cardiac prosthetic valve can occur by contamination at the time of its placement or as a result of hematogenous dissemination or by contiguous infection [2].Staphylococcus aureus is the most common cause of PVE, followed by coagulase-negative staphylococci;Streptococcus viridans group,Enterococcus spp., and yeasts are more common in LO than in EO PVE. Culture-negative endocarditis represents approximately 13% of PVE, with reported rates of 31% [4] and they are more frequent in LO than in EO PVE. Chronic haemodialysis, diabetes, and intravascular devices, including cardiac prosthetic valve, are all predisposing factors toS. aureus endocarditis [24].From a clinical point of view, the presentation of PVE does not substantially differ from the other forms of IE. Indeed, its nonspecific clinical presentation makes IE a cause of fever of unknown origin, with a high clinical suspicion needed to make diagnosis.The modified Duke criteria are considered the gold standard for diagnosing IE [6] (Tables 1, 2, and 3). In epidemiological studies the sensitivity of Duke criteria is 80%, but in clinical practice their diagnostic accuracy is lower, especially in PVE; prosthetic material in fact compromises the sensitivity of echocardiography that results inconclusively in nearly 30% of cases [4].Table 1
Modified Duke criteria for the diagnosis of prosthetic valve endocarditis (PVE) [6].
Definite PVE
Clinical criteria
(1) 2 major criteria
Or
(2) 1 major criterion and 3 minor criteria
Or
(3) 5 minor criteria
Pathological criteria
(1) Microorganisms demonstrated by culture or histological examination of a vegetation, a vegetation that has embolized
Or
(2) An intracardiac abscess specimen or pathological lesions; vegetation or intracardiac abscess confirmed by histological examination showing active endocarditis
Possible PVE
(1) 1 major criterion and 1 minor criterion
Or
(2) 3 minor criteria
Rejected PVE
(1) Firm alternate diagnosis explaining evidence of infective endocarditis
Or
(2) Resolution of infective endocarditis syndrome with antibiotic therapy for ≤4 days
Or
(3) No pathological evidence of infective endocarditis at surgery or autopsy, with antibiotic therapy for ≤4 daysOr
(4) Does not meet criteria for possible infective endocarditis, as aboveTable 2
Major criteria according to Li et al. [6] and new major criteria according to Saby et al. [7] for the diagnosis of prosthetic valve endocarditis (PVE).
Li et al. [6]
Saby et al. [7]
Blood culture positive for infective endocarditis(i) Typical microorganisms consistent with infective endocarditis from 2 separate blood cultures.Viridans streptococci, Streptococcus bovis, HACEK group, Staphylococcus aureus,or community-acquired enterococci, in the absence of a primary focus (ii) Microorganisms consistent with infective endocarditis from persistently positive blood cultures, defined as follows.≥2 positive cultures of blood samples drawn >12 hapart or all of 3 or a majority of ≥4 separate cultures of blood (with first and last sample drawn at least1 hapart) (iii) Single positive blood culture for Coxiella burnetii or antiphase I IgG antibody titer >1 : 800Evidence of endocardial involvement (i) Echocardiogram (TTE and/or TEE) positive for infective endocarditis defined as follows.Oscillating intracardiac mass on valve or supporting structures, in the path of regurgitant jets, or on implanted material in the absence of an alternative anatomic explanation or abscess or new partial dehiscence of prosthetic valve (ii) New valvular regurgitation (worsening or changing of preexisting murmur not sufficient).
Duke major criteriaandpositive 18F-FDG PET/CT: abnormal FDG uptake at the site of prosthetic valve
18F-FDG: 18F-fluorodeoxyglucose; HACEK:Haemophilus species, Actinobacillus actinomycetemcomitans, Cardiobacterium hominis, Eikenella corrodens, and Kingella species; IgG: immunoglobulin G; PET/CT: positron emission tomography/computed tomography; TEE: transesophageal echocardiography; TTE: transthoracic echocardiography.Table 3
Minor criteria for the diagnosis of prosthetic valve endocarditis (PVE) [6].
(i) Predisposition, predisposing heart condition, or injection drug use(ii) Fever, temperature ≥38°C(iii) Vascular phenomena: major arterial emboli, septic pulmonary infarcts, mycotic aneurysm, intracranial hemorrhage, and conjunctival hemorrhages(iv) Janeway lesions(v) Immunological phenomena: glomerulonephritis, Osler nodes, Roth spots, and rheumatoid factor(vi) Microbiological evidence: positive blood culture but does not meet a major criterion as noted above or serological evidence of active infection with organism consistent with infective endocarditisPVE represents up to 30% of IE in developed countries, and the in-hospital mortality in patients with PVE can reach 47%, especially inS. aureus bacteremia; these data confirm that it is important to increase the ability to early diagnose this clinical condition [8].


## 3.1.2. The Contribution of Nuclear Medicine in the Diagnosis of PVE

Several nuclear medicine techniques, characterized by different kind of tracers and technologies, have been implemented in order to offer precious information about the metabolic activity of infected tissue. In the last years, these new imaging modalities have gained an increasing attention because they make it possible to diagnose an IE earlier than the structural alterations occurring.Since the eighties to nowadays several papers have reported the experiences of nuclear medicine (NM) specialists in utilization of NM imaging modalities for PVE diagnosis. Our research provided 29 articles; seven were excluded because they were inadequate to our purpose.Table4 summarizes all techniques mentioned in papers reviewed [7–23]; we did not include 6 commentary articles to the prospective study of Saby et al. that we have already included in Table 4 [25–30].Table 4
Nuclear medicine (NM) in the diagnosis of prosthetic valve endocarditis (PVE): review of the literature.
NM imaging modalities
Number of articles/number of patients
Type of paper/reference/year
18F-FDG-PET/TC
1/not valuable
Review/[8]/2013
1/72
Prospective study/[7]/2013
2/2
Case report/[9, 10]/2013, 2009
1/6
Case series/[11]/2014
CT angiography and18F-FDG-PET
1/7
Case series/[12]/2013
111Indium labeled leukocytes scintigraphy
2/2
Case report/[13, 14]/1990, 1988
1/2
Case series/[15]/1989
67Gallium citrate-SPECT and SPECT/TC
4/4
Case report/[16–19]/2005, 2009, 2002, 1991
99mTechnetium-HMPAO-WBC-SPECT/TC
1/131
Prospective study/[20]/2012
1/42
Prospective study/[21]/2013
99mTechnetium anti-G mAb-SPECT
1/1
Case report/[22]/1991
1/38
Prospective study/[23]/1995
FDG: fluorodeoxyglucose; PET/TC: positron emission tomography/computed tomography; CT: computed tomography; SPECT/TC: single photon emission computed tomography/computed tomography; HMPAO: hexamethylpropylene amine oxime; WBC: white blood cells; anti-G mAb: anti-human granulocytes antibodies.Ten articles reported experiences regarding labeled leukocytes scintigraphy in PVE diagnosis [13–23]; in some of them this technique was associated with single photon emission computed tomography (SPECT) acquisition of images [16–19], because SPECT can provide accurate morphologic details.Six papers regard18F-fluorodeoxyglucose positron emission tomography in association with computed tomography (18F-FDG PET/CT) or with computed tomography angiography (CTA) in the diagnostic assessment of PVE or perivalvular infective complications in patient affected by PVE [12–21].(1)
18
F-FDG PET/CT. 18F-FDG PET/CT combines the assessment of metabolic activity of tissues (namely, the glycolytic activity) with the spatial resolution of CT.A review published in 2013 has extensively collected the available data, mainly from case reports and case series, on the efficacy and usefulness of this technique and has stressed that there is currently no evidence from case-control studies comparing PET/CT with other diagnostic tools currently available [8].To date there has been only one published prospective study that aims to investigate the diagnostic power of this technique [7]. Seventy-two patients with suspected PVE, initially assessed by using the modified Duke criteria, underwent PET/CT. Although the diagnosis of endocarditis was not always confirmed by histological examination of the prosthetic valve, the authors point out that all those cases of endocarditis undergoing surgery and confirmed by pathologists were identified by 18F-FDG PET/CT [7].Sensitivity and specificity reported in this study (73% and 80%, resp.) did not differ greatly from the values found in a recent study on the use of18F-FDG PET/CT for the diagnosis of endocarditis on pacemakers and implantable defibrillators where sensitivity and specificity were 63% and 86, respectively [31].However, this study provides interesting data on the possible inclusion of18F-FDG PET/CT among the major criteria of Duke, as already proposed by Millar et al. [7, 8] (Table 2). In fact, the sensitivity of these “new” modified Duke criteria rises up to 97%, which would increase the rate of the so-called “defined” endocarditis [7].New diagnostic algorithms have been proposed in order to ensure an early diagnosis of endocarditis in those cases in which the Duke criteria are inconclusive; this happens in case of culture-negative endocarditis or in those cases in which transthoracic echocardiography (TTE) and transesophageal echocardiography (TEE) do not visualize lesions of the valve [7, 8].In this regard, in a case report, Gouriet et al. describe the representative contribution of PET/CT to the diagnosis of PVE in case of “possible endocarditis,” according to the Duke criteria. In this study, PET/CT was performed in course of antibiotic therapy for a knee prostheses infection, after 3 TEEs repeated 7 days apart from each other because of a strong suspicion of IE. The new findings of mitral regurgitation and prolapse of one of the mitral cusps at the third TEE have led to the diagnosis of possible endocarditis; the PET TC subsequently performed made it possible to diagnose a definitive PVE [9].New imaging modalities, as computed tomography angiography (CTA), have been proposed as a supplement to PET, as Tanis et al. reported in their case series [12]. The authors describe 7 cases of PVE, where the integration of the two techniques allowed diagnosing PVE unidentified by TEE, and periannular complication, that is, mycotic aneurism of coronary arteries and myocardial abscesses [12].There are still some controversies about various issues related to the PET/CT methodology.First, the resolving power of this technique permits the detection of lesions of 5 mm and over. However, according to some authors, this is not a limit to the diagnostic power as even smaller lesions, if there is a sufficient uptake, can be displayed. In this regard, Bertagna and colleagues observed that the standard protocol that is used in most nuclear medicine centers is the oncological one that consists of imaging acquisition performed 1 hour after glucose injection [26]. Therefore, they suggest acquiring images even at 2-3 hours after the injection of glucose in order to detect uptake by monocytes and macrophages at the level of the lesion and to increase the sensitivity of the PET/CT methodology [26].Moreover, there is concern about the specificity of the method in the early period after prosthesis placement. A functional glucose uptake persists in fact from 1 month up to 1 year after surgery. This variability between individuals is influenced by various factors, such as age, clinical status, and overall cardiac condition of the patient. Some authors have also expressed concern about the lack of controls in the prospective study published by Saby et al. [7], because, in order to correctly interpret the values of uptake and the validity of the exam, it is necessary to compare with a normal pattern of glucose uptake, in the absence of infection [30].(2) Labeled Leukocytes Scintigraphy. 111Indium is one of the first radiopharmaceuticals used in detecting cardiovascular infections (Table 4) [12–14]. Articles collected [12–14] describe scintigraphy with 111Indium employed in diagnosing prosthetic aortic perivalvular abscesses that is a severe endocarditis complication undetectable with traditional radiological tools. This complication is common; it complicates PVE in approximately 50% of cases and needs an urgent surgical approach [20].111Indium seems more efficacious to detect perivalvular complications than valvular surface infections, but its time-consuming images’ acquisition limits its utilization.67Gallium citrate has been used more often than 111Indium as tracer for scintigraphy in cardiovascular infection field, and it has always been associated with SPECT views [16–19]. One of the articles regarding 67Gallium citrate reports its capability to detect endocarditis in absence of any morphological alteration at echocardiography [18].A retrospective study assessed the value of 99mTc-HMPAO- (hexamethylpropylene amine oxime-) WBC (white blood cells) scintigraphy including SPECT/CT acquisitions in a series of 131 consecutive patients with suspected IE, 68% of whom with prosthetic valves [20].99mTc-HMPAO-WBC scintigraphy results were correlated with transthoracic or transesophageal echocardiography, blood cultures, and the Duke criteria.Technetium99m-HMPAO-SPECT/CT was positive in all cases of IE in which at least one major Duke criterion was present; moreover, it was positive in IE cases, ascertained by microbiological or clinical diagnosis, with a negative echocardiography [20].Technetium
99
m labelled monoclonal anti-human granulocytes antibodies (Anti-G mAb) may be employed to label leukocytes in vivo to detect inflammation and infection, but literature regarding its contribution to PVE diagnosis is scarce. Our literature search provided two papers reporting, respectively, a case report and a small prospective case-control study [22, 23]. In this latter article sensitivity and specificity of scintigraphy, in combination with echocardiography, were 100% and 80%, respectively, but the clinical sample was limited, that is, 18 cases and 20 controls [23]. Currently there are no sufficient evidences to suggest this technique as a reliable tool in PVE diagnosis.


## 3.1.3. The Contribution of Nuclear Medicine to the Diagnosis of Complications in IE, Including PVE

Complications of PVE are rare but worrying because they often require an urgent surgical intervention. They include septic embolism, perivalvular infection, myocardial abscesses, and prosthetic valve dehiscence.In this regard a retrospective study provided interesting data about the usefulness ofTechnetium99m-HMPAO-SPECT/CT in assessment of cardiac surgery indications in patients with a suspect PVE [21].With this technique the authors diagnosed extensive perivalvular infections and abscesses in patients with confirmed endocarditis and no perivalvular extension of the infection detected by TEE; surgery confirmed the presence of an abscess in those patients with positive scintigraphy who underwent surgical intervention, with a positive predictive value for the presence of an abscess of 100% [21].It is well known that nuclear medicine contributes to the diagnosis of septic metastatic foci in cases of systemic infections, as reported by Vos et al. in a prospective study regarding 115 patients affected by bacteremia [32].Embolization and/or metastatic infection occurs in more than half of patients with IE and most commonly involves the spleen, the kidney, the liver, and the iliac or mesenteric arteries [33].Embolic events have been reported in 22–43% of patients with IE, mainly in the first 2 weeks after initiation of antibiotic therapy. A misdiagnosed septic embolism can cause a subsequent seeding of the infection at the metastatic site, a persistent bacteremia, and colonization of newly placed prosthetic valve [34].Metastatic infection can occur with different form, for example, septic arthritis, spondylodiscitis, pericarditis, and abscess [33, 35]. Their presence influences the appropriate choice of antibiotic therapy and surgical management, the duration of followup, and the outcome of IE [34]. The contribution of 18F-FDG PET/CT to the early diagnosis of embolism and metastatic infection in patients with IE has been reported in recent prospective studies [34, 36]; peripheral embolism and/or metastatic infection were detected in 44% of IE episodes, 40% of which were PVE [34].Moreover, PET/CT detected unexpected extracardiac sites of infection, that is, asymptomatic cases, in 24%–28% of IE [34, 36].Finally, a retrospective study by Erba et al. on theTechnetium99m-HMPAO-SPECT/CT contribution to the assessment of suspected IE reported the usefulness of this technique for the identification of septic emboli, detected in 41% of patients, even in the absence of the typical echocardiographic predictors of systemic embolism [20].


## 4. Conclusions

The early diagnosis of PVE represents a challenge for clinicians involved in its management, including infectious diseases specialist, cardiologist, cardiosurgeons, and imaging physicians, because a delay in antibiotic therapy and cardiac surgery has negative effects on clinical outcomes.Promising NM techniques, as18F-FDG PET/CT and Technetium99m-HMPAO-SPECT/CT, have been demonstrated to be effective imaging options in the management of PVE. PET has been proposed as a method to increase the sensitivity of the Duke criteria for PVE, and Technetium99m-HMPAO-SPECT/CT is a promising technique that requires further studies. Finally, NM techniques can identify important complications in the course of PVE, including perivalvular abscesses and metastatic embolization, even in asymptomatic patients.



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