# Sustainable Algae Biodiesel Production in Cold Climates

Authors: Rudras Baliga; Susan E. Powers
Journal: International Journal of Chemical Engineering
 (2010)

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## Abstract

This life cycle assessment aims to determine the most suitable operating conditions for algae biodiesel production in cold climates to minimize energy consumption and environmental impacts. Two hypothetical photobioreactor algae production and biodiesel plants located in Upstate New York (USA) are modeled. The photobioreactor is assumed to be housed within a greenhouse that is located adjacent to a fossil fuel or biomass power plant that can supply waste heat and flue gas containingCO2 as a primary source of carbon. Model results show that the biodiesel areal productivity is high (19 to 25 L of BD/m2/yr). The total life cycle energy consumption was between 15 and 23 MJ/L of algae BD and 20 MJ/L of soy BD. Energy consumption and air emissions for algae biodiesel are  substantially lower than soy biodiesel when waste heat was utilized. Algae's most substantial contribution is a significant decrease in the petroleum consumed to make the fuel.


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## Body

## 1. Introduction

In 1998, an amendment to the U.S. Energy Policy Act (EP Act) of 1992 triggered the rapid expansion of the US biodiesel industry. This act required that a fraction of new vehicles purchased by federal and state governments be alternative fuel vehicles. The U.S. Energy Independence and Security Act (EISA) of 2007 further mandated the production of renewable fuels to 36 billion gallons (136 billion liters) per year by 2022, including biodiesel. Crops such as soybeans and canola account for more than three quarters of all biodiesel feedstocks in the U.S. [1].About 14% of U.S. soybean production and 4% of global soybean production were used by the U.S. biodiesel industry to produce fuel in 2007 [1]. The use of oil crops for fuel has been criticized because the expansion of biodiesel production in the United States and Europe has coincided with a sharp increase in prices for food grains and vegetable oils [2]. The production of biodiesel from feedstocks that do not use arable land can be accomplished either by using biomass that is currently treated as waste or by introducing a new technology that allows for the development of new feedstocks for biodiesel that utilize land that is unsuitable for food production.Microalgae have the potential to displace other feedstocks for biodiesel owing to its high vegetable oil content and biomass production rates [3]. The vegetable oil content of algae can vary with growing conditions and species, but has been known to exceed 70% of the dry weight of algae biomass [4]. Microalgae could have significant social and environmental benefits because they do not compete for arable land with food crops and microalgae cultivation consumes less water than other crops [5]. Algae also grow in saline waters that are unsuitable for agricultural practices or consumption. This makes algae well suited for areas where cultivation of other crops is difficult [6, 7]. High biomass productivities may be achieved with indoor or outdoor photobioreactors (PBRs) [8]. In cold climates, PBRs have been used successfully, when housed within greenhouses and provided with artificial lightingMicroalgae biodiesel has received much attention in news media. Considerable progress has been made in the field of algae biomorphology [9–11]. In recent decades, however, little quantitative research has been done on the energy and environmental impacts of microalgae biodiesel production on a life cycle basis. The life cycle concept is a cradle to grave systems approach for the study of feedstocks, production, and use. The objective of this work was to assess the feasibility of algae biodiesel production in New York State (USA) based on life cycle energy and environmental impact parameters. Upstate NY was chosen as a challenging case for algae biodiesel production due to shorter days and cold temperatures during winter months. The productivity, energy consumption, and environmental emissions associated with the algae/BD production lifecycle were quantified in order to identify the best growing conditions and assess its impacts relative to soybean biodiesel.


## 2. Methodology

### 2.1. System Boundary and Scope

The life cycle concept is a cradle to grave systems approach for the study of feedstocks, production, and use. The concept revolves around the recognition of different stages of production starting from upstream use of energy to cultivation of the feedstock, followed by the different processing stages. A life cycle inventory assessment allows for the quantification of mass and energy streams such as energy consumption, material usage, waste production, and generation of coproducts. A summary of the sustainability assessment metrics used for this life cycle inventory of microalgae feedstock for biodiesel production is presented in Table1.Table 1
Life cycle sustainability metrics for biodiesel.
Environmental ImpactSustainability MetricsEnergy and Resource Consumption– Total energy consumed (MJ/L BD)– Fossil fuel energy consumed (MJ/L BD)– Petroleum consumed (MJ/L BD)– Land required (m2/L of BD)– Water required (L water/L BD)Climate Change– Net green house gas emissions (g CO2 equivalents/L of BD)Acidification– Acidification potential (g SO2 eq./L BD)Toxic Emissions– Particulate matter emissions (PM10, PM2.5)– Carbon monoxide emissions– Volatile organic carbon emissionsFigure1 provides an overview of the system boundary used in this analysis, which includes the production of algae and biodiesel via a transesterification reaction. The boundary includes all upstream mass and energy flows that are required to make the chemical and energy resources required for the processing. The production of biodiesel from algae and direct energy consumption is characterized by four distinct stages: cultivation, dewatering/drying, oil extraction, and transesterification (Figure 1). The energy consumed and subsequent emissions for fuel production, electricity generation, and chemical production comprise the upstream energy consumption and emissions. Biodiesel and algae meal are the products leaving the system boundary. The use of these products is not directly included within the analysis.Figure 1
Flowchart depicting system boundary for life cycle inventory of biodiesel from microalgae.The hypothetical algae and biodiesel production processing facilities considered are located in upstate placeStateNew York. The facilities are assumed to be adjacent to a biomass or fossil fuel electricity generation plant for access to the carbon dioxide in their flue gas and waste heat in order to maximize the utilization of waste resources within this system. Waste heat is considered to have no value as an energy product; so it is not counted as part of the total energy resources consumed by the facility.Two different locations were considered for the microalgae biodiesel facility: Syracuse, NY (43°2′ N, 76°8′ W) and Albany, NY (42°7′ N, 73°8′ W). Although these locations are at approximately the same latitude and have very similar hours of daylight, the Syracuse area is colder and cloudier throughout the year due to its proximity to the Great Lakes. Albany offers more intense natural lighting and less severe winter temperatures (Figure 2). Three specific cases were considered for each of these locations:(i)
greenhouse structure to maximize natural lighting; natural gas used to maintain the system temperature;(ii)
greenhouse structure to maximize natural lighting; waste heat used to maintain the system temperature;(iii)
a well-insulated facility that allows for no natural lighting but requires substantially less heat.Monthly average temperature (a) and total monthly solar irradiance (b) for Syracuse, NY, and Albany, NY.
(a)(b)The PBRs are assumed to operate continuously, using artificial lighting when natural lighting is not sufficient. In all cases, it was assumed thatPhaeodactylum tricornutum algae would be grown for biodiesel production. This algae species has a relatively high oil content (about 30% by dry weight), is resistant to contamination, and has been previously utilized to produce biodiesel [12, 13].Estimating the environmental and energy lifecycle impacts requires quantification of the mass and energy flows through this system. A mathematical model for the algae production process was developed in the work presented here. As shown in Figure3, the mass and energy flows estimated with the algae production model were used in conjunction with the Greenhouse gases, Related Emissions, and Energy use in Transportation (GREET) model 1.8a developed at the Argonne National Laboratories [14]. GREET provided the general framework and structure for the lifecycle inventory, especially aspects of the transesterification process and energy and emissions related to the upstream production of chemicals and energy resources. BD production from soybeans, which is used here as a benchmark for comparison, was taken directly from the GREET model. GREET is a widely accepted model and many studies and analyses have been based upon it because of its vast data on energy sources and the associated emissions (e.g., [15–17]). The default values for soybean production, oil extraction, and transesterification were taken as GREET default values [14], which are representative of the Midwestern region of the United States where most soybeans are grown. These were based initially on an LCA completed at the National Renewable Energy Lab [18] and updated to keep the GREET model as current as possible (e.g., [17]). There is only a small production of soybeans in NY State, with yields well below the average yield in the Midwest. Thus, no attempt was made to match the geographic system boundaries for biodiesel from algae to that of soybeans.Figure 3
Overview of microalgae biomass LCA model. The yellow boxes represent the contributions of the work presented here. The soybean LCA results were taken almost entirely from GREET.Uncertainty in the data was addressed by utilizing Monte Carlo simulations to input a range of values for parameters. For a given assumption or variable with a distribution as input, the commercially available software, Crystal Ball was utilized to determine a forecast or range of possible outputs. Standard error bars were created utilizing the mean value of the forecast and 95% certainty.


### 2.2. Algae Production Models

The biomass production model utilizes solar data and a biological growth rate to estimate actual yields for algae biomass for a photobioreactor system [13, 19].


#### 2.2.1. Microalgae Plant Setup

Hypothetical tubular closed photobioreactors (PBRs) were modeled in this case to predict algae production and account for energy consumption and emissions in Syracuse and Albany NY. The PBR plant setup is illustrated in Figure4. It was assumed that processes such as dewatering and transesterification could be carried out on site, thus eliminating the need for transportation.Figure 4
Photobioreactor system layout (not to scale).The various dimensions and parameters for the PBR were taken from recommendations of previous studies in order to depict a realistic setup [12, 13]. The PBR setup was designed for a maximum of 30 hours detention time. The maximum effluent concentration (Ce) was fixed at 3.4 kg/m3 with a recycle ratio of 0.35 [13, 20] and an allowable superficial fluid velocity of 0.3 m/s [21]. Since a long tubular length is required to meet these constraints (32,400 m), the PBR is split up into 6 units each of which is 61 m3 (5,400 m long, 0.12 m diameter). Stacking of tubes reduces the total foot print area of the greenhouse. All tubes are connected and algae broth passes through all six units.The floor area or foot print area of the greenhouse house was determined from the volume of the reactor and type of cultivation (annual/seasonal operation) and the specific processes. The diameter of tubes was set at 0.12 m for all cases since it is a widely reported size for PBRs [3, 13, 22, 23]. The spacing of tubes was set at 0.3 m. This is an important factor since it defines the total floor size, which in turn influences heating and lighting requirements. The various parameters related to plant setup are summarized in Table 2.Table 2
Summary of photobioreactor and greenhouse parameters.
ParameterValueDepends uponReferenceDiameter of tubes0.12 mLarger diameter pipes can cause cell shading[3]Spacing of tubes0.3Greater spacing is desirable to avoid shading[13]Flow rate (Q)3.4×10-3 m3/sAlgae species and tube diameter[21]Recycle Ratio (r)0.35[20]Total volume of reactor (V)366m3Maximum residence time (30 hrs)[12]Influent concentration (Ci)1.2 kg/m3Max. Effluent concentration (Ce)3.4 kg/m3Growth rate, PBR set up[13]


#### 2.2.2. Estimating Biomass Output

Microalgae productivity is estimated from the location, reactor specifications, and microalgae data. It is assumed thatCO2 and nutrients are provided in excess to the microalgae culture through the media, thereby making light the only limiting factor for cell growth and decay [12]. If adequate lighting is available, the specific growth rate μ is determined from the average irradiance available Iavg (μE/m2-s) [19]: (1)μ=μmaxIavgnKIn+Iavgn,
where KI is the half saturation constant (i.e., Iavg for which half of μmax is attained), and the exponent n is a unitless empirical constant. Both KI and n are constant for a given species of algae. Note that decay of algae cells during the hours with light is incorporated into the maximum specific growth rate (μmax) (h-1) since values provided by Molina Grima et al. [22] and Fernandez et al. [23] were determined from the net growth rate. Iavg is determined from the Beer-Lambert equation:(2)Iavg=IφeqKaCi[1-exp(-φeqKaCi)],
where Ci (kg/m3) is the influent biomass concentration. The path length of light within the reactor is given by φeq, which is the ratio of the tube diameter to the cosine of the solar zenith angle. The photosynthetically active irradiance (I) (μEm-2s-1) is a function of various solar angles and the total solar irradiance. Hourly solar data were available from NREL’s solar database [24], and thus, algae cell growth was determined at an interval of an hour. The analysis of the solar data to estimate I and φeq is included in the appendix.The PBR is modeled as a series of plug flow reactors where the effluent concentration of each reactor is the influent concentration for the next. It is assumed that steady-state conditions prevail for each hour since irradiance is taken as a constant over that time period. Utilizing a Monod reaction rate for substrate utilization [22], the resulting steady-state plug flow reactor equation for each segment can be written as(3)udCdz=μmaxIavgnKIn+IavgnC,
where C is the biomass concentration and u is the fluid velocity. Integrating this expression can provide the effluent concentration for each reactor segment “j” that represents one hour of residence time at an average irradiation rate for that hour:(4)ln(Cj+1kCjk)=μmax(Iavg(j+1)k)nKIn+(Iavg(j+1)k)n(Lu),
where j and j+1 indicate the start and end location along the reactor length of the one hour segment. Cj+1 is calculated in series to determine the reactor effluent concentration, Ce, for each hour k. The growth rate during that hour is defined by the average irradiance for that hour of the day. The total biomass produced per day (MBM) (kg d-1) is estimated from the flow rate (Q) (m3hr-1), recycle ratio (r), and the effluent concentration Ce (kg m-3):(5)MBM=∑k=124Q(Cek)(1-r).
The total microalgae biomass produced can be determined from (2), (4), and (5) along with algae growth parameters and solar irradiation data.The temperature requirements for algae differ by species. In general, faster growing algae species favor higher media temperatures of about 20–30°C [10]. The algae-related constants used for P. tricornutum in the model are included in Table 3. This species was selected because it has been used in the past to produce microalgae biodiesel and all relevant data were available [22].Table 3
P. tricornutum growth parameters.
ParameterValueReferenceμmax0.063 (h-1)[22]B0.0018 (h-1)[25]KI3426 (μE m-2s-1)[22]n1–1.34[25]Ef1.74 ± 0.09μE J-1[22]Xp2–4%[25]Oil Content (%dry weight)30%[13]Water Content of algae40%[13]Cultivation temperature25°C[10]


### 2.3. Energy Consumed during Microalgae Cultivation

Cultivating microalgae in closed systems is an energy intensive process, especially in regions with low temperatures and limited natural lighting [13]. The algae growth and harvesting stage involve a large number of intermediate processes for which estimates of the energy consumption were developed here. Energy consumption requirements for extraction and transesterification are already provided in the GREET model [14]. It was assumed that the processes for transesterification of algae oil are identical to that of soy oil. Thus, the chemical (methanol and sodium hydroxide) and energy consumption and the energy and emissions associated with their production were taken directly as the default parameters in GREET.Water Heating
Energy requirements for a natural gas water heating system were determined by using the specific heat of water and the efficiency rating of the heater as provided by the manufacturer (EF = 0.82 [26]). It was assumed that groundwater, at an initial temperature (Tinlet) of 12°C [27], would be heated to a thermostat set point (Tt) of 25°C.Media Circulation
Electric pumps are used to circulate the media through the entire length of the reactor. The input electrical power required to operate the pumps(Pp) (W) can be given by [28]
(6)Pp=3.91×10-6μ13Re2.75ηpd3Aa,whereRe=ρudμ1,
where μ1 (kg m-1s-1) is the dynamic viscosity of water, Re is the Reynolds number, d (m) is the diameter of the pipes, u is the superficial velocity of flow (m/s), ηp and ηelec are the pump efficiency (ηp = 0.7), and Aa (m2)is the tube aperture area. The pumps operate continuously.Artificial Lighting
Natural algae cultivation inherently revolves around the diurnal and seasonal cycles. To compensate for these cycles and to maximize the production of biomass, artificial lighting is used to allow 24-hour cultivation. Lights are turned on from dusk to dawn. Monthly averages of daylight hours are used to define the time the lighting system is in operation each day. The power(Pa) (W) consumed for artificially lighting the greenhouse area is calculated as [29]
(7)Pa=A′(IavgLwCf).
The intensity of the artificial lighting provided was set equal to the naturally available lighting in the month of July (Iavg = 1.7 μE/m2-s) over the entire greenhouse region (A′=3345m2). Specifications for high-efficiency fluorescent GRO lights [29] were used to estimate the power required for artificial lighting. The light intensity of the bulbs is expressed as Lw = 220 Lu/W and the conversion factor (Cf) to convert between micromoles of photons (mE) and lux is 0.29.CO2 Purification
Carbon dioxide acts as the only source of carbon for the biomass. Flue gases from power plants provide an inexhaustible source ofCO2. However, flue gases also contain varying levels of other gases such as SOx and NOx which are detrimental to microalgae culture beyond certain concentrations [30]. The monoethanolamine (MEA) absorption process can be used to separate pure CO2 from flue gas for microalgae production. Kadam [31] determined that if about 18% of the total carbon dioxide consumed is taken directly from flue gases and the rest is purified through the MEA process, then, the toxic flue gases will be sufficiently low concentration for algae growth.
Molina Grima [19] determined that in order to make light the only limiting factor, CO2 must be provided in excess and the ratio of the aqueous CO2 concentration (kg/m3) to influent biomass concentration Ci (kg/m3) should be 0.63. Since growth rates for this system are lower than those in Molina's Grima study [19] due to reduced sunlight, this CO2 represents a conservative estimate. The mass of carbon dioxide required was estimated based on this ratio, the media flow rate, and the influent biomass concentration. Although carbon dioxide has a high solubility in water, and it is likely that all CO2 in the gas bubbled through the reactor would dissolve over the length of the reactor, a factor of safety of 2 was used here as an overestimate of the mass of CO2 that would be required. The MEA CO2 extraction process has been modeled and studied previously in context with algae production. Kadam [31, 32] reports that the process to extract CO2 from flue gas and recover the MEA for reuse consumes 32.65 kWh per ton of CO2 for algae cultivation. Details are not provided in these references to specifically quantify which of the steps in the MEA process consume the most electrical energy.Greenhouse Heating
Temperature control within the greenhouse is essential for algae cultivation in cold weather conditions. The energy consumed for greenhouse heating depends upon the total surface area exposed, insulation material, and temperature inside and outside the greenhouse. For a given greenhouse with surface area(Ag) (m2) the heat loss per second (QL) (J/s) is given by [33]
(8)QL=1.05(1R)(Treq-Tout)Ag,
where R(1.9 m2C° s J-1) is the R-value of the greenhouse insulating material; Treq (25°C) and Tout (°C)are the temperatures required within the greenhouse and outside the greenhouse, respectively. The greenhouse was assumed to be insulated with 10 mm twinwall polycarbonate with an R-value of 1.9. The R-value of insulated and windowless cultivation scenario was set at 30. The outside temperature Tout is taken from monthly averages for Syracuse and Albany and is input as normal distributions for that month [34].Steam Drying and Dewatering
Algae are suspended in a dilute broth from photobioreactors [13]. Dewatering and drying of algae is necessary to reduce the water content to 5% [35] before the hexane oil extraction process. For algae with high vegetable oil content, it is suggested that continuous nozzle discharge centrifuges provide the best reliability and consume the least amount of energy. Centrifugation consumes 3.24 MJ/m3 of effluent media [36]. After centrifugation, algae water content is 70% (by weight).
Steam is utilized to further dry microalgae before oil extraction process. The natural gas consumed to provide the required steam energy was calculated based on the heat of vaporization of water, the mass of water that needed to be vaporized to reduce the water content from 0.70 to 0.05, and the efficiency of the boiler (0.93 [37]) and dryer (0.8 [26]) utilized. In the scenarios that utilize waste heat, it is assumed that because of the colocation of the algae production facility near a power plant, there is sufficient heat to dry the algae.


### 2.4. Water Consumption

The consumption of water for the production of biofuels has recently been identified as a significant limitation to the development of an expanded biofuel economy. Water consumption occurs almost entirely in the feedstock production step for most biofuels. The average U.S. production biodiesel from soybeans requires 6,500 liters of water for evapotranspiration per liter of biodiesel produced [38]. Water consumption for algae biodiesel was calculated by a mass balance. The total water flowrate through the bioreactor is the sum of freshwater, water included in the algae recycle stream (35% recycle), and water recovered through the centrifuge dewatering process to increase the algae concentration from 0.34% to 30%. With this mass balance, 848 m3 make up water is required annually or approximately 4 L water per L of biodiesel for the feedstock production stage. This represents approximately 99% of water recovery and reuse. In the transesterification and biodiesel cleaning processes, 1–3 L of water are required per L of biodiesel produced [39].


### 2.5. Fertilizer Consumption

The microalgae culture media acts as the primary source of nutrients and carbon dioxide and a means of expelling excess oxygen. The minimum amount of nutrients consumed was defined based on the molecular formula of algae—CO0.48H1.83N0.11P0.01 [40]. N and P account for 6.5% and 1.3% of the algae mass. Assuming that maximum possible biomass concentration of algae cells is 4 kg /m3 [13, 22] in a tubular PBR, the N and P consumed from the algae media would be 0.26 kg N/m3 and 0.052 kg P/m3. Excess fertilizer that passes through the bioreactor as part of the broth is assumed to be recovered in the centrifuge dewatering step for reuse. Since nearly all of the water is recycled, it is assumed that nearly all of the nutrients that are not consumed are also recycled.


### 2.6. Utilizing GREET for Life Cycle Analysis

The GREET model was modified and used to calculate the energy use and emissions generated from algae production, oil extraction, and transesterification stages of biodiesel production as well as the upstream chemical and energy production processes. For a given fuel system GREET evaluates natural gas, coal, and petroleum use as well as the emissions of carbon dioxide equivalent greenhouse gases, volatile organic compounds, carbon monoxide, nitrogen oxides, particulates, and sulfur oxides from all lifecycle stages [14]. The GREET results are presented as primary energy consumed and emissions per million BTU fuel produced. The low heating value of the BD was used to convert to the functional unit used here—liters BD produced.The GREET model is written in an MS Excel workbook and includes soy biodiesel production energy consumption and emissions pathways. A new spreadsheet page based on the soy biodiesel calculations was added to the GREET workbook and adapted for algae BD production. Default parameters for transesterification were used directly, but other input parameters including energy consumption for the various processes, biomass yield, nutrient requirements, carbon dioxide consumed were modified for algae biodiesel production based on the mass and energy flows presented above. The mix of electricity generation within New York State was used to define the primary energy consumed to generate electricity [41].The extraction of oil from algae was assumed to be carried out by hexane oil extraction. The procedure is similar to soybean oil extraction, although significantly less hexane is required to recover oil from algae (0.030 kg of hexane/kg of dry algae) [11] than is required for soybeans (1.2 kg hexane/kg dried and flaked soy bean) [18]. During this process, algae meal is produced as a coproduct that can be used as an animal feed in the same manner that soy meal is used as a coproduct from soy biodiesel. GREET uses the displacement method to determine how much of the biomass production and extraction steps can defined as a credit for the biodiesel due to the production of a coproduct. The protein content of soy meal is 48% [42], as compared to 28% in algae meal [13] and 40% in soy beans [42]. Thus, 1 kg of algae meal displaces about 0.7 kg of soybean, whereas 1 kg of soy meal displaces about 1.2 kg of soy bean for animal feed. The credits for not having to produce 0.7 kg soy beans for every kg algae meal produced are subtracted from the total energy use and emissions associated with the algae production, oil extraction, and their associated upstream processes.An additional credit was also attributed to the algae to represent the carbon dioxide sequestered from the power plant flue gas. Algae cell elemental composition was used to estimate the mass of carbon that was consumed by the algae growth within the PBR (0.51 kg ofCO2 consumed/kg algae grown).


## 2.1. System Boundary and Scope

The life cycle concept is a cradle to grave systems approach for the study of feedstocks, production, and use. The concept revolves around the recognition of different stages of production starting from upstream use of energy to cultivation of the feedstock, followed by the different processing stages. A life cycle inventory assessment allows for the quantification of mass and energy streams such as energy consumption, material usage, waste production, and generation of coproducts. A summary of the sustainability assessment metrics used for this life cycle inventory of microalgae feedstock for biodiesel production is presented in Table1.Table 1
Life cycle sustainability metrics for biodiesel.
Environmental ImpactSustainability MetricsEnergy and Resource Consumption– Total energy consumed (MJ/L BD)– Fossil fuel energy consumed (MJ/L BD)– Petroleum consumed (MJ/L BD)– Land required (m2/L of BD)– Water required (L water/L BD)Climate Change– Net green house gas emissions (g CO2 equivalents/L of BD)Acidification– Acidification potential (g SO2 eq./L BD)Toxic Emissions– Particulate matter emissions (PM10, PM2.5)– Carbon monoxide emissions– Volatile organic carbon emissionsFigure1 provides an overview of the system boundary used in this analysis, which includes the production of algae and biodiesel via a transesterification reaction. The boundary includes all upstream mass and energy flows that are required to make the chemical and energy resources required for the processing. The production of biodiesel from algae and direct energy consumption is characterized by four distinct stages: cultivation, dewatering/drying, oil extraction, and transesterification (Figure 1). The energy consumed and subsequent emissions for fuel production, electricity generation, and chemical production comprise the upstream energy consumption and emissions. Biodiesel and algae meal are the products leaving the system boundary. The use of these products is not directly included within the analysis.Figure 1
Flowchart depicting system boundary for life cycle inventory of biodiesel from microalgae.The hypothetical algae and biodiesel production processing facilities considered are located in upstate placeStateNew York. The facilities are assumed to be adjacent to a biomass or fossil fuel electricity generation plant for access to the carbon dioxide in their flue gas and waste heat in order to maximize the utilization of waste resources within this system. Waste heat is considered to have no value as an energy product; so it is not counted as part of the total energy resources consumed by the facility.Two different locations were considered for the microalgae biodiesel facility: Syracuse, NY (43°2′ N, 76°8′ W) and Albany, NY (42°7′ N, 73°8′ W). Although these locations are at approximately the same latitude and have very similar hours of daylight, the Syracuse area is colder and cloudier throughout the year due to its proximity to the Great Lakes. Albany offers more intense natural lighting and less severe winter temperatures (Figure 2). Three specific cases were considered for each of these locations:(i)
greenhouse structure to maximize natural lighting; natural gas used to maintain the system temperature;(ii)
greenhouse structure to maximize natural lighting; waste heat used to maintain the system temperature;(iii)
a well-insulated facility that allows for no natural lighting but requires substantially less heat.Monthly average temperature (a) and total monthly solar irradiance (b) for Syracuse, NY, and Albany, NY.
(a)(b)The PBRs are assumed to operate continuously, using artificial lighting when natural lighting is not sufficient. In all cases, it was assumed thatPhaeodactylum tricornutum algae would be grown for biodiesel production. This algae species has a relatively high oil content (about 30% by dry weight), is resistant to contamination, and has been previously utilized to produce biodiesel [12, 13].Estimating the environmental and energy lifecycle impacts requires quantification of the mass and energy flows through this system. A mathematical model for the algae production process was developed in the work presented here. As shown in Figure3, the mass and energy flows estimated with the algae production model were used in conjunction with the Greenhouse gases, Related Emissions, and Energy use in Transportation (GREET) model 1.8a developed at the Argonne National Laboratories [14]. GREET provided the general framework and structure for the lifecycle inventory, especially aspects of the transesterification process and energy and emissions related to the upstream production of chemicals and energy resources. BD production from soybeans, which is used here as a benchmark for comparison, was taken directly from the GREET model. GREET is a widely accepted model and many studies and analyses have been based upon it because of its vast data on energy sources and the associated emissions (e.g., [15–17]). The default values for soybean production, oil extraction, and transesterification were taken as GREET default values [14], which are representative of the Midwestern region of the United States where most soybeans are grown. These were based initially on an LCA completed at the National Renewable Energy Lab [18] and updated to keep the GREET model as current as possible (e.g., [17]). There is only a small production of soybeans in NY State, with yields well below the average yield in the Midwest. Thus, no attempt was made to match the geographic system boundaries for biodiesel from algae to that of soybeans.Figure 3
Overview of microalgae biomass LCA model. The yellow boxes represent the contributions of the work presented here. The soybean LCA results were taken almost entirely from GREET.Uncertainty in the data was addressed by utilizing Monte Carlo simulations to input a range of values for parameters. For a given assumption or variable with a distribution as input, the commercially available software, Crystal Ball was utilized to determine a forecast or range of possible outputs. Standard error bars were created utilizing the mean value of the forecast and 95% certainty.


## 2.2. Algae Production Models

The biomass production model utilizes solar data and a biological growth rate to estimate actual yields for algae biomass for a photobioreactor system [13, 19].


### 2.2.1. Microalgae Plant Setup

Hypothetical tubular closed photobioreactors (PBRs) were modeled in this case to predict algae production and account for energy consumption and emissions in Syracuse and Albany NY. The PBR plant setup is illustrated in Figure4. It was assumed that processes such as dewatering and transesterification could be carried out on site, thus eliminating the need for transportation.Figure 4
Photobioreactor system layout (not to scale).The various dimensions and parameters for the PBR were taken from recommendations of previous studies in order to depict a realistic setup [12, 13]. The PBR setup was designed for a maximum of 30 hours detention time. The maximum effluent concentration (Ce) was fixed at 3.4 kg/m3 with a recycle ratio of 0.35 [13, 20] and an allowable superficial fluid velocity of 0.3 m/s [21]. Since a long tubular length is required to meet these constraints (32,400 m), the PBR is split up into 6 units each of which is 61 m3 (5,400 m long, 0.12 m diameter). Stacking of tubes reduces the total foot print area of the greenhouse. All tubes are connected and algae broth passes through all six units.The floor area or foot print area of the greenhouse house was determined from the volume of the reactor and type of cultivation (annual/seasonal operation) and the specific processes. The diameter of tubes was set at 0.12 m for all cases since it is a widely reported size for PBRs [3, 13, 22, 23]. The spacing of tubes was set at 0.3 m. This is an important factor since it defines the total floor size, which in turn influences heating and lighting requirements. The various parameters related to plant setup are summarized in Table 2.Table 2
Summary of photobioreactor and greenhouse parameters.
ParameterValueDepends uponReferenceDiameter of tubes0.12 mLarger diameter pipes can cause cell shading[3]Spacing of tubes0.3Greater spacing is desirable to avoid shading[13]Flow rate (Q)3.4×10-3 m3/sAlgae species and tube diameter[21]Recycle Ratio (r)0.35[20]Total volume of reactor (V)366m3Maximum residence time (30 hrs)[12]Influent concentration (Ci)1.2 kg/m3Max. Effluent concentration (Ce)3.4 kg/m3Growth rate, PBR set up[13]


### 2.2.2. Estimating Biomass Output

Microalgae productivity is estimated from the location, reactor specifications, and microalgae data. It is assumed thatCO2 and nutrients are provided in excess to the microalgae culture through the media, thereby making light the only limiting factor for cell growth and decay [12]. If adequate lighting is available, the specific growth rate μ is determined from the average irradiance available Iavg (μE/m2-s) [19]: (1)μ=μmaxIavgnKIn+Iavgn,
where KI is the half saturation constant (i.e., Iavg for which half of μmax is attained), and the exponent n is a unitless empirical constant. Both KI and n are constant for a given species of algae. Note that decay of algae cells during the hours with light is incorporated into the maximum specific growth rate (μmax) (h-1) since values provided by Molina Grima et al. [22] and Fernandez et al. [23] were determined from the net growth rate. Iavg is determined from the Beer-Lambert equation:(2)Iavg=IφeqKaCi[1-exp(-φeqKaCi)],
where Ci (kg/m3) is the influent biomass concentration. The path length of light within the reactor is given by φeq, which is the ratio of the tube diameter to the cosine of the solar zenith angle. The photosynthetically active irradiance (I) (μEm-2s-1) is a function of various solar angles and the total solar irradiance. Hourly solar data were available from NREL’s solar database [24], and thus, algae cell growth was determined at an interval of an hour. The analysis of the solar data to estimate I and φeq is included in the appendix.The PBR is modeled as a series of plug flow reactors where the effluent concentration of each reactor is the influent concentration for the next. It is assumed that steady-state conditions prevail for each hour since irradiance is taken as a constant over that time period. Utilizing a Monod reaction rate for substrate utilization [22], the resulting steady-state plug flow reactor equation for each segment can be written as(3)udCdz=μmaxIavgnKIn+IavgnC,
where C is the biomass concentration and u is the fluid velocity. Integrating this expression can provide the effluent concentration for each reactor segment “j” that represents one hour of residence time at an average irradiation rate for that hour:(4)ln(Cj+1kCjk)=μmax(Iavg(j+1)k)nKIn+(Iavg(j+1)k)n(Lu),
where j and j+1 indicate the start and end location along the reactor length of the one hour segment. Cj+1 is calculated in series to determine the reactor effluent concentration, Ce, for each hour k. The growth rate during that hour is defined by the average irradiance for that hour of the day. The total biomass produced per day (MBM) (kg d-1) is estimated from the flow rate (Q) (m3hr-1), recycle ratio (r), and the effluent concentration Ce (kg m-3):(5)MBM=∑k=124Q(Cek)(1-r).
The total microalgae biomass produced can be determined from (2), (4), and (5) along with algae growth parameters and solar irradiation data.The temperature requirements for algae differ by species. In general, faster growing algae species favor higher media temperatures of about 20–30°C [10]. The algae-related constants used for P. tricornutum in the model are included in Table 3. This species was selected because it has been used in the past to produce microalgae biodiesel and all relevant data were available [22].Table 3
P. tricornutum growth parameters.
ParameterValueReferenceμmax0.063 (h-1)[22]B0.0018 (h-1)[25]KI3426 (μE m-2s-1)[22]n1–1.34[25]Ef1.74 ± 0.09μE J-1[22]Xp2–4%[25]Oil Content (%dry weight)30%[13]Water Content of algae40%[13]Cultivation temperature25°C[10]


## 2.2.1. Microalgae Plant Setup

Hypothetical tubular closed photobioreactors (PBRs) were modeled in this case to predict algae production and account for energy consumption and emissions in Syracuse and Albany NY. The PBR plant setup is illustrated in Figure4. It was assumed that processes such as dewatering and transesterification could be carried out on site, thus eliminating the need for transportation.Figure 4
Photobioreactor system layout (not to scale).The various dimensions and parameters for the PBR were taken from recommendations of previous studies in order to depict a realistic setup [12, 13]. The PBR setup was designed for a maximum of 30 hours detention time. The maximum effluent concentration (Ce) was fixed at 3.4 kg/m3 with a recycle ratio of 0.35 [13, 20] and an allowable superficial fluid velocity of 0.3 m/s [21]. Since a long tubular length is required to meet these constraints (32,400 m), the PBR is split up into 6 units each of which is 61 m3 (5,400 m long, 0.12 m diameter). Stacking of tubes reduces the total foot print area of the greenhouse. All tubes are connected and algae broth passes through all six units.The floor area or foot print area of the greenhouse house was determined from the volume of the reactor and type of cultivation (annual/seasonal operation) and the specific processes. The diameter of tubes was set at 0.12 m for all cases since it is a widely reported size for PBRs [3, 13, 22, 23]. The spacing of tubes was set at 0.3 m. This is an important factor since it defines the total floor size, which in turn influences heating and lighting requirements. The various parameters related to plant setup are summarized in Table 2.Table 2
Summary of photobioreactor and greenhouse parameters.
ParameterValueDepends uponReferenceDiameter of tubes0.12 mLarger diameter pipes can cause cell shading[3]Spacing of tubes0.3Greater spacing is desirable to avoid shading[13]Flow rate (Q)3.4×10-3 m3/sAlgae species and tube diameter[21]Recycle Ratio (r)0.35[20]Total volume of reactor (V)366m3Maximum residence time (30 hrs)[12]Influent concentration (Ci)1.2 kg/m3Max. Effluent concentration (Ce)3.4 kg/m3Growth rate, PBR set up[13]


## 2.2.2. Estimating Biomass Output

Microalgae productivity is estimated from the location, reactor specifications, and microalgae data. It is assumed thatCO2 and nutrients are provided in excess to the microalgae culture through the media, thereby making light the only limiting factor for cell growth and decay [12]. If adequate lighting is available, the specific growth rate μ is determined from the average irradiance available Iavg (μE/m2-s) [19]: (1)μ=μmaxIavgnKIn+Iavgn,
where KI is the half saturation constant (i.e., Iavg for which half of μmax is attained), and the exponent n is a unitless empirical constant. Both KI and n are constant for a given species of algae. Note that decay of algae cells during the hours with light is incorporated into the maximum specific growth rate (μmax) (h-1) since values provided by Molina Grima et al. [22] and Fernandez et al. [23] were determined from the net growth rate. Iavg is determined from the Beer-Lambert equation:(2)Iavg=IφeqKaCi[1-exp(-φeqKaCi)],
where Ci (kg/m3) is the influent biomass concentration. The path length of light within the reactor is given by φeq, which is the ratio of the tube diameter to the cosine of the solar zenith angle. The photosynthetically active irradiance (I) (μEm-2s-1) is a function of various solar angles and the total solar irradiance. Hourly solar data were available from NREL’s solar database [24], and thus, algae cell growth was determined at an interval of an hour. The analysis of the solar data to estimate I and φeq is included in the appendix.The PBR is modeled as a series of plug flow reactors where the effluent concentration of each reactor is the influent concentration for the next. It is assumed that steady-state conditions prevail for each hour since irradiance is taken as a constant over that time period. Utilizing a Monod reaction rate for substrate utilization [22], the resulting steady-state plug flow reactor equation for each segment can be written as(3)udCdz=μmaxIavgnKIn+IavgnC,
where C is the biomass concentration and u is the fluid velocity. Integrating this expression can provide the effluent concentration for each reactor segment “j” that represents one hour of residence time at an average irradiation rate for that hour:(4)ln(Cj+1kCjk)=μmax(Iavg(j+1)k)nKIn+(Iavg(j+1)k)n(Lu),
where j and j+1 indicate the start and end location along the reactor length of the one hour segment. Cj+1 is calculated in series to determine the reactor effluent concentration, Ce, for each hour k. The growth rate during that hour is defined by the average irradiance for that hour of the day. The total biomass produced per day (MBM) (kg d-1) is estimated from the flow rate (Q) (m3hr-1), recycle ratio (r), and the effluent concentration Ce (kg m-3):(5)MBM=∑k=124Q(Cek)(1-r).
The total microalgae biomass produced can be determined from (2), (4), and (5) along with algae growth parameters and solar irradiation data.The temperature requirements for algae differ by species. In general, faster growing algae species favor higher media temperatures of about 20–30°C [10]. The algae-related constants used for P. tricornutum in the model are included in Table 3. This species was selected because it has been used in the past to produce microalgae biodiesel and all relevant data were available [22].Table 3
P. tricornutum growth parameters.
ParameterValueReferenceμmax0.063 (h-1)[22]B0.0018 (h-1)[25]KI3426 (μE m-2s-1)[22]n1–1.34[25]Ef1.74 ± 0.09μE J-1[22]Xp2–4%[25]Oil Content (%dry weight)30%[13]Water Content of algae40%[13]Cultivation temperature25°C[10]


## 2.3. Energy Consumed during Microalgae Cultivation

Cultivating microalgae in closed systems is an energy intensive process, especially in regions with low temperatures and limited natural lighting [13]. The algae growth and harvesting stage involve a large number of intermediate processes for which estimates of the energy consumption were developed here. Energy consumption requirements for extraction and transesterification are already provided in the GREET model [14]. It was assumed that the processes for transesterification of algae oil are identical to that of soy oil. Thus, the chemical (methanol and sodium hydroxide) and energy consumption and the energy and emissions associated with their production were taken directly as the default parameters in GREET.Water Heating
Energy requirements for a natural gas water heating system were determined by using the specific heat of water and the efficiency rating of the heater as provided by the manufacturer (EF = 0.82 [26]). It was assumed that groundwater, at an initial temperature (Tinlet) of 12°C [27], would be heated to a thermostat set point (Tt) of 25°C.Media Circulation
Electric pumps are used to circulate the media through the entire length of the reactor. The input electrical power required to operate the pumps(Pp) (W) can be given by [28]
(6)Pp=3.91×10-6μ13Re2.75ηpd3Aa,whereRe=ρudμ1,
where μ1 (kg m-1s-1) is the dynamic viscosity of water, Re is the Reynolds number, d (m) is the diameter of the pipes, u is the superficial velocity of flow (m/s), ηp and ηelec are the pump efficiency (ηp = 0.7), and Aa (m2)is the tube aperture area. The pumps operate continuously.Artificial Lighting
Natural algae cultivation inherently revolves around the diurnal and seasonal cycles. To compensate for these cycles and to maximize the production of biomass, artificial lighting is used to allow 24-hour cultivation. Lights are turned on from dusk to dawn. Monthly averages of daylight hours are used to define the time the lighting system is in operation each day. The power(Pa) (W) consumed for artificially lighting the greenhouse area is calculated as [29]
(7)Pa=A′(IavgLwCf).
The intensity of the artificial lighting provided was set equal to the naturally available lighting in the month of July (Iavg = 1.7 μE/m2-s) over the entire greenhouse region (A′=3345m2). Specifications for high-efficiency fluorescent GRO lights [29] were used to estimate the power required for artificial lighting. The light intensity of the bulbs is expressed as Lw = 220 Lu/W and the conversion factor (Cf) to convert between micromoles of photons (mE) and lux is 0.29.CO2 Purification
Carbon dioxide acts as the only source of carbon for the biomass. Flue gases from power plants provide an inexhaustible source ofCO2. However, flue gases also contain varying levels of other gases such as SOx and NOx which are detrimental to microalgae culture beyond certain concentrations [30]. The monoethanolamine (MEA) absorption process can be used to separate pure CO2 from flue gas for microalgae production. Kadam [31] determined that if about 18% of the total carbon dioxide consumed is taken directly from flue gases and the rest is purified through the MEA process, then, the toxic flue gases will be sufficiently low concentration for algae growth.
Molina Grima [19] determined that in order to make light the only limiting factor, CO2 must be provided in excess and the ratio of the aqueous CO2 concentration (kg/m3) to influent biomass concentration Ci (kg/m3) should be 0.63. Since growth rates for this system are lower than those in Molina's Grima study [19] due to reduced sunlight, this CO2 represents a conservative estimate. The mass of carbon dioxide required was estimated based on this ratio, the media flow rate, and the influent biomass concentration. Although carbon dioxide has a high solubility in water, and it is likely that all CO2 in the gas bubbled through the reactor would dissolve over the length of the reactor, a factor of safety of 2 was used here as an overestimate of the mass of CO2 that would be required. The MEA CO2 extraction process has been modeled and studied previously in context with algae production. Kadam [31, 32] reports that the process to extract CO2 from flue gas and recover the MEA for reuse consumes 32.65 kWh per ton of CO2 for algae cultivation. Details are not provided in these references to specifically quantify which of the steps in the MEA process consume the most electrical energy.Greenhouse Heating
Temperature control within the greenhouse is essential for algae cultivation in cold weather conditions. The energy consumed for greenhouse heating depends upon the total surface area exposed, insulation material, and temperature inside and outside the greenhouse. For a given greenhouse with surface area(Ag) (m2) the heat loss per second (QL) (J/s) is given by [33]
(8)QL=1.05(1R)(Treq-Tout)Ag,
where R(1.9 m2C° s J-1) is the R-value of the greenhouse insulating material; Treq (25°C) and Tout (°C)are the temperatures required within the greenhouse and outside the greenhouse, respectively. The greenhouse was assumed to be insulated with 10 mm twinwall polycarbonate with an R-value of 1.9. The R-value of insulated and windowless cultivation scenario was set at 30. The outside temperature Tout is taken from monthly averages for Syracuse and Albany and is input as normal distributions for that month [34].Steam Drying and Dewatering
Algae are suspended in a dilute broth from photobioreactors [13]. Dewatering and drying of algae is necessary to reduce the water content to 5% [35] before the hexane oil extraction process. For algae with high vegetable oil content, it is suggested that continuous nozzle discharge centrifuges provide the best reliability and consume the least amount of energy. Centrifugation consumes 3.24 MJ/m3 of effluent media [36]. After centrifugation, algae water content is 70% (by weight).
Steam is utilized to further dry microalgae before oil extraction process. The natural gas consumed to provide the required steam energy was calculated based on the heat of vaporization of water, the mass of water that needed to be vaporized to reduce the water content from 0.70 to 0.05, and the efficiency of the boiler (0.93 [37]) and dryer (0.8 [26]) utilized. In the scenarios that utilize waste heat, it is assumed that because of the colocation of the algae production facility near a power plant, there is sufficient heat to dry the algae.


## 2.4. Water Consumption

The consumption of water for the production of biofuels has recently been identified as a significant limitation to the development of an expanded biofuel economy. Water consumption occurs almost entirely in the feedstock production step for most biofuels. The average U.S. production biodiesel from soybeans requires 6,500 liters of water for evapotranspiration per liter of biodiesel produced [38]. Water consumption for algae biodiesel was calculated by a mass balance. The total water flowrate through the bioreactor is the sum of freshwater, water included in the algae recycle stream (35% recycle), and water recovered through the centrifuge dewatering process to increase the algae concentration from 0.34% to 30%. With this mass balance, 848 m3 make up water is required annually or approximately 4 L water per L of biodiesel for the feedstock production stage. This represents approximately 99% of water recovery and reuse. In the transesterification and biodiesel cleaning processes, 1–3 L of water are required per L of biodiesel produced [39].


## 2.5. Fertilizer Consumption

The microalgae culture media acts as the primary source of nutrients and carbon dioxide and a means of expelling excess oxygen. The minimum amount of nutrients consumed was defined based on the molecular formula of algae—CO0.48H1.83N0.11P0.01 [40]. N and P account for 6.5% and 1.3% of the algae mass. Assuming that maximum possible biomass concentration of algae cells is 4 kg /m3 [13, 22] in a tubular PBR, the N and P consumed from the algae media would be 0.26 kg N/m3 and 0.052 kg P/m3. Excess fertilizer that passes through the bioreactor as part of the broth is assumed to be recovered in the centrifuge dewatering step for reuse. Since nearly all of the water is recycled, it is assumed that nearly all of the nutrients that are not consumed are also recycled.


## 2.6. Utilizing GREET for Life Cycle Analysis

The GREET model was modified and used to calculate the energy use and emissions generated from algae production, oil extraction, and transesterification stages of biodiesel production as well as the upstream chemical and energy production processes. For a given fuel system GREET evaluates natural gas, coal, and petroleum use as well as the emissions of carbon dioxide equivalent greenhouse gases, volatile organic compounds, carbon monoxide, nitrogen oxides, particulates, and sulfur oxides from all lifecycle stages [14]. The GREET results are presented as primary energy consumed and emissions per million BTU fuel produced. The low heating value of the BD was used to convert to the functional unit used here—liters BD produced.The GREET model is written in an MS Excel workbook and includes soy biodiesel production energy consumption and emissions pathways. A new spreadsheet page based on the soy biodiesel calculations was added to the GREET workbook and adapted for algae BD production. Default parameters for transesterification were used directly, but other input parameters including energy consumption for the various processes, biomass yield, nutrient requirements, carbon dioxide consumed were modified for algae biodiesel production based on the mass and energy flows presented above. The mix of electricity generation within New York State was used to define the primary energy consumed to generate electricity [41].The extraction of oil from algae was assumed to be carried out by hexane oil extraction. The procedure is similar to soybean oil extraction, although significantly less hexane is required to recover oil from algae (0.030 kg of hexane/kg of dry algae) [11] than is required for soybeans (1.2 kg hexane/kg dried and flaked soy bean) [18]. During this process, algae meal is produced as a coproduct that can be used as an animal feed in the same manner that soy meal is used as a coproduct from soy biodiesel. GREET uses the displacement method to determine how much of the biomass production and extraction steps can defined as a credit for the biodiesel due to the production of a coproduct. The protein content of soy meal is 48% [42], as compared to 28% in algae meal [13] and 40% in soy beans [42]. Thus, 1 kg of algae meal displaces about 0.7 kg of soybean, whereas 1 kg of soy meal displaces about 1.2 kg of soy bean for animal feed. The credits for not having to produce 0.7 kg soy beans for every kg algae meal produced are subtracted from the total energy use and emissions associated with the algae production, oil extraction, and their associated upstream processes.An additional credit was also attributed to the algae to represent the carbon dioxide sequestered from the power plant flue gas. Algae cell elemental composition was used to estimate the mass of carbon that was consumed by the algae growth within the PBR (0.51 kg ofCO2 consumed/kg algae grown).


## 3. Results

### 3.1. Biomass Production

Biomass output is an important factor for determining life cycle energy analysis of microalgae biodiesel production. When natural lighting is used to minimize electricity consumption for artificial lighting, algae production rises steadily between the months of February and April (Figure5). Biomass production is the highest between the months of May to July and is followed by a gradual decline in the months of August to October. Production is the lowest in the winter months due to low natural irradiance. The uncertainty bars included represent 95% confidence intervals from Monte Carlo simulation outputs.Figure 5
Algae Biomass production for Syracuse and Albany, NY, with natural lighting supplemented by artificial lighting for continuous algae production.The annual biomass productivity in Albany is about 12% greater than that in Syracuse (Table4). These cities are at very similar latitudes, but the actual irradiance in Albany is higher due to less cloud cover. Biomass and subsequent biodiesel production in the windowless (artificial lighting only) scenario is much higher than greenhouse cases because illumination is maintained throughout the year at the highest level achieved naturally (noon in the month of July).Table 4
Comparison of different locations and scenarios by biodiesel production.
LocationBiomass Produced(tonnes/year)Biodiesel produced (Lm-2 y-1)Syracuse NYGreenhouse Base Case20219Greenhouse w/waste heat20219Albany NYWindowless Cultivation26325Greenhouse Base Case22521Greenhouse w/waste heat22521


### 3.2. Energy Consumption for Microalgae Biodiesel Production

The energy consumed for biodiesel production was estimated by modeling individual processes in the algae cultivation stage. Energy required for the transesterification process is accounted directly by the GREET 1.8a model. The energy required for feedstock production through the drying process is illustrated in Figure6. This does not include oil extraction and transesterification processes. Three variables can be assessed with this graph: location (Syracuse versus Albany), use of natural lighting versus solely artificial lighting and algae versus soybean production.Figure 6
Energy consumption for microalgae and soy bean feedstock production. The error bars represent 95% confidence intervals on the total energy consumption for feedstock production.Heating needs consume well over half of the total energy required for algae growth, dewatering, and drying. When no waste heat is available, dewatering and steam drying accounts for the greatest fraction—about 28–32% of the energy required for feedstock production. With the availability of waste heat, this component is reduced to about 13% of the total, which represents the electricity required for centrifugation. Greenhouse heating consumes a similar proportion of the total energy for algae production—about 25–30%. Water heating for cultivation consumes about 7–12% for feedstock production. Both locations have similar water heating requirements because groundwater temperature is assumed to be equal for both cases.When natural lighting is utilized to the extent possible artificial lighting, it consumes about a quarter of the total energy required for algae cultivation. However, in the windowless cultivation case where there is no natural light available, the artificial lighting cost is almost doubled. However, the total energy requirements in this scenario are still less (35%) than the scenarios requiring natural gas to heat a greenhouse.Among the design choices and trade-offs considered here, the growth and drying of algae with the utilization of waste heat is the only scenario that is substantially better than growing soybeans from the perspective of process energy consumed. These results clearly show the value of colocating an algae facility near a source of waste heat.Overall, microalgae cultivation in Albany, NY, consumes about 18–21% less energy than Syracuse, NY, because greenhouse heating energy requirements are lower and higher natural lighting intensity yields about 12% higher biomass output.Figure7 illustrates the total lifecycle energy, which now also includes biodiesel production and credits for CO2 consumption and algae/soy meal produced during the oil extraction phase. For most cases, the energy required for feedstock production is similar to the energy required for oil extraction and transesterification. Thus, the savings associated with the utilization of waste heat in the greenhouse also represent significant savings when the entire lifecycle energy consumption is considered. Greenhouse algae cultivation with waste heat in Albany consumes the least energy on a life cycle basis; however total energy consumption is very similar to that of the corresponding Syracuse case.Figure 7
Total life cycle energy consumption by life cycle stage. The error bars represent 95% confidence intervals on the total lifecycle energy consumed.The importance of the coproduct and carbon dioxide consumption credits are apparent from the data presented in Figure7. Soy meal credits are higher than algae meal credits because of higher protein content and higher fraction of soy meal produced per liter of biodiesel (1 kg of algae meal displaces about 0.7 kg of soybean, whereas 1 kg of soy meal displaces about 1.2 kg of soy bean for animal feed). Adding the higher credits for the soy bean BD case to the energy required for production reduces the net energy for this case to a level below the well-insulated and windowless algae production scenario. The greenhouse scenarios utilizing waste heat are still the best option for minimizing the consumption of energy that has value for other uses.Natural gas accounts for 65–80% of the total energy consumed on a life cycle basis for algae biodiesel production when waste heat is not available (data not shown). The high consumption of natural gas can be attributed to heating processes, the high fraction of natural gas in NY electricity mix (about 22%), and upstream consumption for process fuel and fertilizer production. In contrast, soy biodiesel requires substantially more petroleum (~5x) than microalgae consumes due to the extensive use of tractors and feedstock transportation when BD is made from soybeans. Thus, algae as a BD feedstock has a significant benefit over soybeans in terms of reducing our dependence on imported oil. Algae biodiesel production requires a significant amount of electricity and thus coal accounts for about 6–19% of the total life cycle energy consumption. Insulated cultivation has the highest coal consumption, about 19% of the total life cycle energy consumption, because of increased artificial lighting and electricity consumption. In comparison, for the greenhouse with waste heat case, only 7% the total lifecycle energy is derived from coal.The processing of soybeans to prepare for oil extraction also requires some heating to dry the beans. Arguably, waste heat could be considered to reduce the fossil fuel consumption for soybean biodiesel too. However, whereas the algae feedstock could be grown at the same location where waste heat is available, the soybeans require a much more dispersed geographical region. Soybeans are typically transported 75 miles or less to a soybean crushing facility. Thus, the probability that soybean production and crushing facilities can be colocated with a waste heat source is significantly less than for algae. If this can be achieved, the lifecycle energy production for the feedstock production (green bar for soybean BD, Figure7) would be less.


### 3.3. Global Warming Potential

Global warming potential can be described as the impact of additional units of greenhouse gases to the atmosphere. The global warming potential for the different scenarios and gases is estimated in terms of carbon dioxide equivalents (Figure8). All algae scenarios are allocated the same CO2 credits because the carbon dioxide consumed per unit of algae produced is constant.Figure 8
Global warming potential of microalgae biodiesel—mass emissions normalized by dividing by the corresponding emissions for soy biodiesel for comparison.MostCO2 emissions for algae biodiesel originate from upstream usage of energy use for heating, transportation fuel use, and coal combustion for electricity. The extraction and utilization of natural gas for heating use, electricity generation, and fertilizer production is accompanied by high methane emissions. Natural gas extraction has a very high methane emission factor. Overall, the emission of carbon dioxide is relatively low compared to methane due to the high natural gas use relative to petroleum or coal. Natural gas utilization has a much lower carbon dioxide emission factor than coal.In cold climates, the production of algae biodiesel with the utilization of waste heat rather than natural gas consumption is the only approach that reduces greenhouse gas emissions relative to soy biodiesel.


### 3.4. Other Air Emissions

The exposure of humans to air pollutants is increasingly associated with increased mortality and reduction in life expectancy [43]. Figure 9 presents the lifecycle air emissions for algae biodiesel production normalized to the corresponding air emissions estimated by GREET for soybean biodiesel. The microalgae biodiesel air emissions follow a trend similar to the total life cycle energy consumption. The high NOx emissions can be traced to high emission factors of equipment used to produce natural gas and the flaring of natural gas in refineries. The increased use of artificial lighting for the cultivation of algae in a windowless and well-insulated facility results in high particulate emissions, particularly in comparison to cases where natural lighting is used. These PM emissions originate mainly from coal and residual oil combustion use for electricity production.Figure 9
Toxic air emissions from microalgae biodiesel production—mass emissions normalized by dividing by the corresponding emissions for soy biodiesel for comparison.VOC emissions from microalgae biodiesel production are much lower than soy biodiesel, because of low utilization of petroleum and hexane. The VOC emission factors for transportation fuels like gasoline are far greater than any other source. Thus, since algae is locally produced for biodiesel and transportation minimal, the VOC emissions from algae biodiesel are much less than soy biodiesel, primarily because only a minimal amount of hexane is required for extraction compared with soy beans.Overall, the most important source of air emissions for microalgae is the upstream emissions associated with fuel and electricity generation. Yet, these emissions are still relatively low compared to soy biodiesel. The primary factor contributing to this apparent anomaly is the comparison of algae biodiesel produced in New York State to soy biodiesel produced nationally. NY State has a high percentage of hydroelectric (17%) and nuclear (29%) power production and relatively small amounts of electricity generated from coal (15%) [41]. This difference in upstream electricity generation has significant repercussions throughout the lifecycle energy emission estimates for any electricity-intensive manufacturing system: manufacturing in New York State benefits from relatively clean energy resources.The acidification of soils and water bodies occurs mainly due to the transformation of gaseous pollutants (SOx, NOx) into acids. The acidification potential of the different cases is estimated in SO2 equivalents. All cases of microalgae biodiesel are better than soy biodiesel in terms of acidification emissions. The total SO2 equivalents follow a trend that resembles the total energy usage.


### 3.5. Summary of Results

A summary of the lifecycle sustainability assessment metrics for the various algae biodiesel production scenarios and soy biodiesel production is presented in Table5. The most sustainable biodiesel production for all cases requires the colocation of the algae and BD production facility in the vicinity of a source of waste heat. “Free” heat greatly reduces the fossil fuel consumption and all related green house gas and other air pollutants. At a similar latitude, choosing a location that maximizes sunlight helps somewhat to increase the algae production rate and, therefore, reduce the impacts when the results are compared on a per BD-produced basis. These effects are small, however, compared to the benefits of utilizing waste heat. Similarly, a well-insulated facility can help reduce heating needs, but the consequences of increased electricity use for artificial lighting decrease the benefits of reduced heating fuel required. In most regions of the U.S., where a higher fraction of the electricity mix is generated from fossil fuels, the well insulated windowless scenario would be worse in terms of most sustainability metrics due to the increased dependence on fossil fuels.Table 5
Summary of average sustainability metrics to compare algae and soy BD production.
ScenarioEnvironmental ImpactGreenhouse Nat. Gas, SyracuseGreenhouse Nat. Gas, AlbanyGreenhouse w/waste heat, SyracuseGreenhouse w/waste heat, AlbanyInsulated, no nat. lightSoy biodiesel productionTotal life cycle energy Consumption* (MJ/L of BD)232116152221Land utilization (m2/L of BD/yr)0.0530.0480.0530.0480.04022.2Water Consumption (L water/L BD)5–75–75–75–74–66,500Greenhouse gas emissions (g CO2 equiv/L of BD)13501150740630910925Acidification potential (g SO2 eq./L of BD)4.94.62.82.53.44.0Toxic Emissions (g /L of BD)PM 105.14.62.62.35.75.3PM 2.51.81.60.70.61.82.7VOC0.220.200.060.050.093.4CO2.42.10.60.51.02.8*does not include credits.


## 3.1. Biomass Production

Biomass output is an important factor for determining life cycle energy analysis of microalgae biodiesel production. When natural lighting is used to minimize electricity consumption for artificial lighting, algae production rises steadily between the months of February and April (Figure5). Biomass production is the highest between the months of May to July and is followed by a gradual decline in the months of August to October. Production is the lowest in the winter months due to low natural irradiance. The uncertainty bars included represent 95% confidence intervals from Monte Carlo simulation outputs.Figure 5
Algae Biomass production for Syracuse and Albany, NY, with natural lighting supplemented by artificial lighting for continuous algae production.The annual biomass productivity in Albany is about 12% greater than that in Syracuse (Table4). These cities are at very similar latitudes, but the actual irradiance in Albany is higher due to less cloud cover. Biomass and subsequent biodiesel production in the windowless (artificial lighting only) scenario is much higher than greenhouse cases because illumination is maintained throughout the year at the highest level achieved naturally (noon in the month of July).Table 4
Comparison of different locations and scenarios by biodiesel production.
LocationBiomass Produced(tonnes/year)Biodiesel produced (Lm-2 y-1)Syracuse NYGreenhouse Base Case20219Greenhouse w/waste heat20219Albany NYWindowless Cultivation26325Greenhouse Base Case22521Greenhouse w/waste heat22521


## 3.2. Energy Consumption for Microalgae Biodiesel Production

The energy consumed for biodiesel production was estimated by modeling individual processes in the algae cultivation stage. Energy required for the transesterification process is accounted directly by the GREET 1.8a model. The energy required for feedstock production through the drying process is illustrated in Figure6. This does not include oil extraction and transesterification processes. Three variables can be assessed with this graph: location (Syracuse versus Albany), use of natural lighting versus solely artificial lighting and algae versus soybean production.Figure 6
Energy consumption for microalgae and soy bean feedstock production. The error bars represent 95% confidence intervals on the total energy consumption for feedstock production.Heating needs consume well over half of the total energy required for algae growth, dewatering, and drying. When no waste heat is available, dewatering and steam drying accounts for the greatest fraction—about 28–32% of the energy required for feedstock production. With the availability of waste heat, this component is reduced to about 13% of the total, which represents the electricity required for centrifugation. Greenhouse heating consumes a similar proportion of the total energy for algae production—about 25–30%. Water heating for cultivation consumes about 7–12% for feedstock production. Both locations have similar water heating requirements because groundwater temperature is assumed to be equal for both cases.When natural lighting is utilized to the extent possible artificial lighting, it consumes about a quarter of the total energy required for algae cultivation. However, in the windowless cultivation case where there is no natural light available, the artificial lighting cost is almost doubled. However, the total energy requirements in this scenario are still less (35%) than the scenarios requiring natural gas to heat a greenhouse.Among the design choices and trade-offs considered here, the growth and drying of algae with the utilization of waste heat is the only scenario that is substantially better than growing soybeans from the perspective of process energy consumed. These results clearly show the value of colocating an algae facility near a source of waste heat.Overall, microalgae cultivation in Albany, NY, consumes about 18–21% less energy than Syracuse, NY, because greenhouse heating energy requirements are lower and higher natural lighting intensity yields about 12% higher biomass output.Figure7 illustrates the total lifecycle energy, which now also includes biodiesel production and credits for CO2 consumption and algae/soy meal produced during the oil extraction phase. For most cases, the energy required for feedstock production is similar to the energy required for oil extraction and transesterification. Thus, the savings associated with the utilization of waste heat in the greenhouse also represent significant savings when the entire lifecycle energy consumption is considered. Greenhouse algae cultivation with waste heat in Albany consumes the least energy on a life cycle basis; however total energy consumption is very similar to that of the corresponding Syracuse case.Figure 7
Total life cycle energy consumption by life cycle stage. The error bars represent 95% confidence intervals on the total lifecycle energy consumed.The importance of the coproduct and carbon dioxide consumption credits are apparent from the data presented in Figure7. Soy meal credits are higher than algae meal credits because of higher protein content and higher fraction of soy meal produced per liter of biodiesel (1 kg of algae meal displaces about 0.7 kg of soybean, whereas 1 kg of soy meal displaces about 1.2 kg of soy bean for animal feed). Adding the higher credits for the soy bean BD case to the energy required for production reduces the net energy for this case to a level below the well-insulated and windowless algae production scenario. The greenhouse scenarios utilizing waste heat are still the best option for minimizing the consumption of energy that has value for other uses.Natural gas accounts for 65–80% of the total energy consumed on a life cycle basis for algae biodiesel production when waste heat is not available (data not shown). The high consumption of natural gas can be attributed to heating processes, the high fraction of natural gas in NY electricity mix (about 22%), and upstream consumption for process fuel and fertilizer production. In contrast, soy biodiesel requires substantially more petroleum (~5x) than microalgae consumes due to the extensive use of tractors and feedstock transportation when BD is made from soybeans. Thus, algae as a BD feedstock has a significant benefit over soybeans in terms of reducing our dependence on imported oil. Algae biodiesel production requires a significant amount of electricity and thus coal accounts for about 6–19% of the total life cycle energy consumption. Insulated cultivation has the highest coal consumption, about 19% of the total life cycle energy consumption, because of increased artificial lighting and electricity consumption. In comparison, for the greenhouse with waste heat case, only 7% the total lifecycle energy is derived from coal.The processing of soybeans to prepare for oil extraction also requires some heating to dry the beans. Arguably, waste heat could be considered to reduce the fossil fuel consumption for soybean biodiesel too. However, whereas the algae feedstock could be grown at the same location where waste heat is available, the soybeans require a much more dispersed geographical region. Soybeans are typically transported 75 miles or less to a soybean crushing facility. Thus, the probability that soybean production and crushing facilities can be colocated with a waste heat source is significantly less than for algae. If this can be achieved, the lifecycle energy production for the feedstock production (green bar for soybean BD, Figure7) would be less.


## 3.3. Global Warming Potential

Global warming potential can be described as the impact of additional units of greenhouse gases to the atmosphere. The global warming potential for the different scenarios and gases is estimated in terms of carbon dioxide equivalents (Figure8). All algae scenarios are allocated the same CO2 credits because the carbon dioxide consumed per unit of algae produced is constant.Figure 8
Global warming potential of microalgae biodiesel—mass emissions normalized by dividing by the corresponding emissions for soy biodiesel for comparison.MostCO2 emissions for algae biodiesel originate from upstream usage of energy use for heating, transportation fuel use, and coal combustion for electricity. The extraction and utilization of natural gas for heating use, electricity generation, and fertilizer production is accompanied by high methane emissions. Natural gas extraction has a very high methane emission factor. Overall, the emission of carbon dioxide is relatively low compared to methane due to the high natural gas use relative to petroleum or coal. Natural gas utilization has a much lower carbon dioxide emission factor than coal.In cold climates, the production of algae biodiesel with the utilization of waste heat rather than natural gas consumption is the only approach that reduces greenhouse gas emissions relative to soy biodiesel.


## 3.4. Other Air Emissions

The exposure of humans to air pollutants is increasingly associated with increased mortality and reduction in life expectancy [43]. Figure 9 presents the lifecycle air emissions for algae biodiesel production normalized to the corresponding air emissions estimated by GREET for soybean biodiesel. The microalgae biodiesel air emissions follow a trend similar to the total life cycle energy consumption. The high NOx emissions can be traced to high emission factors of equipment used to produce natural gas and the flaring of natural gas in refineries. The increased use of artificial lighting for the cultivation of algae in a windowless and well-insulated facility results in high particulate emissions, particularly in comparison to cases where natural lighting is used. These PM emissions originate mainly from coal and residual oil combustion use for electricity production.Figure 9
Toxic air emissions from microalgae biodiesel production—mass emissions normalized by dividing by the corresponding emissions for soy biodiesel for comparison.VOC emissions from microalgae biodiesel production are much lower than soy biodiesel, because of low utilization of petroleum and hexane. The VOC emission factors for transportation fuels like gasoline are far greater than any other source. Thus, since algae is locally produced for biodiesel and transportation minimal, the VOC emissions from algae biodiesel are much less than soy biodiesel, primarily because only a minimal amount of hexane is required for extraction compared with soy beans.Overall, the most important source of air emissions for microalgae is the upstream emissions associated with fuel and electricity generation. Yet, these emissions are still relatively low compared to soy biodiesel. The primary factor contributing to this apparent anomaly is the comparison of algae biodiesel produced in New York State to soy biodiesel produced nationally. NY State has a high percentage of hydroelectric (17%) and nuclear (29%) power production and relatively small amounts of electricity generated from coal (15%) [41]. This difference in upstream electricity generation has significant repercussions throughout the lifecycle energy emission estimates for any electricity-intensive manufacturing system: manufacturing in New York State benefits from relatively clean energy resources.The acidification of soils and water bodies occurs mainly due to the transformation of gaseous pollutants (SOx, NOx) into acids. The acidification potential of the different cases is estimated in SO2 equivalents. All cases of microalgae biodiesel are better than soy biodiesel in terms of acidification emissions. The total SO2 equivalents follow a trend that resembles the total energy usage.


## 3.5. Summary of Results

A summary of the lifecycle sustainability assessment metrics for the various algae biodiesel production scenarios and soy biodiesel production is presented in Table5. The most sustainable biodiesel production for all cases requires the colocation of the algae and BD production facility in the vicinity of a source of waste heat. “Free” heat greatly reduces the fossil fuel consumption and all related green house gas and other air pollutants. At a similar latitude, choosing a location that maximizes sunlight helps somewhat to increase the algae production rate and, therefore, reduce the impacts when the results are compared on a per BD-produced basis. These effects are small, however, compared to the benefits of utilizing waste heat. Similarly, a well-insulated facility can help reduce heating needs, but the consequences of increased electricity use for artificial lighting decrease the benefits of reduced heating fuel required. In most regions of the U.S., where a higher fraction of the electricity mix is generated from fossil fuels, the well insulated windowless scenario would be worse in terms of most sustainability metrics due to the increased dependence on fossil fuels.Table 5
Summary of average sustainability metrics to compare algae and soy BD production.
ScenarioEnvironmental ImpactGreenhouse Nat. Gas, SyracuseGreenhouse Nat. Gas, AlbanyGreenhouse w/waste heat, SyracuseGreenhouse w/waste heat, AlbanyInsulated, no nat. lightSoy biodiesel productionTotal life cycle energy Consumption* (MJ/L of BD)232116152221Land utilization (m2/L of BD/yr)0.0530.0480.0530.0480.04022.2Water Consumption (L water/L BD)5–75–75–75–74–66,500Greenhouse gas emissions (g CO2 equiv/L of BD)13501150740630910925Acidification potential (g SO2 eq./L of BD)4.94.62.82.53.44.0Toxic Emissions (g /L of BD)PM 105.14.62.62.35.75.3PM 2.51.81.60.70.61.82.7VOC0.220.200.060.050.093.4CO2.42.10.60.51.02.8*does not include credits.


## 4. Conclusions

Cultivation of microalgae in NY State is an energy intensive process owing to temperature control and steam drying process. Colocating microalgae cultivation with a power plant is highly desirable. Annual production of microalgae requires the utilization of waste heat for steam drying, water heating, and greenhouse heating in order to be substantially better than soy biodiesel in terms of energy consumption and emissions. When waste heat is utilized, microalgae biodiesel production consumes less energy than soy biodiesel.Microalgae consumes less than one third the petroleum fossil fuel required for soy biodiesel and only a small fraction of the water. The feasibility of microalgae biodiesel production at a given location is greatly dependent on availability of waste heat and natural lighting conditions. The availability of either one or both makes algae biodiesel production process cleaner in terms of air emissions and consumes much less energy than soy biodiesel. However if both natural lighting and waste heat are absent, algae biodiesel production consumes more energy than soy biodiesel production and emits an equal or more amount toxic air emissions.Coproducts produced during algae biodiesel production process have less protein content than soy meal and, thus, are less valuable. The production of high value coproducts allows for increased energy allocation for soy biodiesel and thus emissions or energy consumption of both the feedstocks is very close and comparable.Most microalgae biodiesel production scenarios have low or very similar emissions as compared to soy biodiesel. Green house gas emissions for algae biodiesel are generally higher than soy biodiesel except when waste heat is utilized, in which case emissions are equal. The emission of volatile organic compounds for soy biodiesel is much higher than that for algae biodiesel. Emissions from microalgae production originate mainly from upstream fossil fuel energy consumption. Reducing needs for unit processes like greenhouse heating, lighting, and other systems will have significant benefits.



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*Source: 102179-2010-06-09.xml*