# Comparison of the Chromosome Banding Pattern in the2n=56 Cytotypes of Nannospalax leucodon and N. xanthodon from Turkey

Authors: Atilla Arslan; Apdil Arısoy; Jan Zima
Journal: The Scientific World Journal
 (2014)

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## Abstract

We present the karyotype characteristics of five cytotypes of mole rats (Nannospalax) with 56 chromosomes revealed by the C-banding and AgNOR staining analyses. We attempt to investigate if the specific distinction between the populations from Thrace (N. leucodon) and Anatolia (N. xanthodon) is reflected also in their karyotypic differentiation. The specimens from each of the five populations studied revealed a distinct karyotype which was different from those found in other populations. The fundamental number of autosomal arms varied from 68 to 72. The amount of C-heterochromatin was larger in the Thrace sample of N. leucodon compared to the Anatolian population of N. xanthodon. The active NOR sites were recorded on five autosomal pairs in N. leucodon, whereas only three or four pairs bearing NOR were observed in N. xanthodon. Differences between the studied populations were quantified in the analysis of the distribution pattern of the C-positive bands and the AgNOR sites in individual chromosomes which indicated a basal position of the Thrace sample of N. leucodon and its divergence from other studied populations. The karyotypes of the 56-chromosome populations of N. leucodon and N. xanthodon are thus distinctly different.


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## Body

## 1. Introduction

The mole rats (Muridae, Spalacinae) [1] represent an attractive model for various biological and evolutionary studies [2, 3]. The attraction of mole rats is due to several reasons, related particularly to the unusual subterranean way of life and various unique adaptive features in their behaviour, sensory biology, and population ecology. Another remarkable character of mole rats is their exceptionally extensive karyotypic variation which has been recorded between populations. Many geographic populations were shown to possess a distinct chromosomal complement differing from other populations. A population or a group of populations with a characteristic karyotype is commonly called the cytotype or the chromosomal race. Some authors [4] proposed that individual cytotypes may be recognized as presumptively good biological species.The amazing chromosomal variation in the mole rats is not reflected in the extent of morphological differentiation. The external phenotype of mole rats is quite uniform as a consequence of selection constraints resulting apparently from their strictly underground habits. The morphological uniformity is obviously responsible for frequently changing and complicated systematics of this group. Currently, the mole rats are classified in two distinct genera,Spalax and Nannospalax [5]. The extensive intraspecific variation in the karyotype is typical for the latter genus which is usually divided into three species with nearly parapatric distribution. Southeastern Europe is inhabited by N. leucodon, Anatolia and Transcaucasia by N. xanthodon, and other parts of the Middle East and northeastern edges of Africa by N. ehrenbergi[1, 6, 7]. The borders between the distribution ranges of individual species are not clear in details and their morphological discrimination is difficult.Turkey can be considered a core area of differentiation processes in chromosomal evolution within mole rats [8–10] and all the three Nannospalax species were recorded in this country. N. leucodon is presumably restricted to the European part of the country called Thrace and its occurrence in the western parts of Anatolia (the Asiatic part of Turkey) is not sure [7, 11]. The karyotype of Turkish populations of N. leucodoncomprises 56 chromosomes (e.g., [10, 12, 13]), but the same diploid number was recorded also in various populations of N. xanthodon from Anatolia (e.g., [12–16]). In these studies the conventionally chromosomes were mostly studied and the banding data are largely lacking.In the present study we aim to improve karyotypic characteristics of various cytotypes with 56 chromosomes included either inN. leucodon or N. xanthodonby the C-banding and AgNOR staining analyses. We attempt to investigate if the specific distinction between the populations from Thrace and Anatolia is reflected also in their karyotypic differentiation. To answer this question, we compare the results with other reports of chromosomal banding patterns found in Turkish populations of Nannospalax [17–22].


## 2. Material and Methods

Cytogenetic analyses were performed in 15 specimens ofN. leucodonand N. xanthodonfrom five Turkish populations. The specimens were caught with a metal pipe-type trap [23]. This trapping method enables to obtain living individuals without any injury. The number of specimens analyzed and location of the collection sites of mole rats are shown in Figure 1 and Table 1.  The study was done and the specimens were obtained with the permission of Republic of Turkey, Ministry of Forest and Water Works (Permission no. 7468).Table 1
Studied localities ofNannospalax leucodon and N. xanthodon in Turkey. The numbering of the sampling sites corresponds to data in Figure 1.
No.
Species
Locality/province
Latitude/longitude
2n
No. of specimens
NF
NFa
X
Y
Male
Female
1
N. leucodon
Babaeski/Kırklareli
41°25′N, 27°07′E
56
1
1
76
72
M
A
2
N. xanthodon
Kula/Manisa
38°30′N, 28°36′E
56
2
—
72
68
Sm
A
3
N. xanthodon
Alaşehir/Manisa
38°20′N, 28°34′E
56
3
2
74
70
Sm
A
4
N. xanthodon
Yılanlı/Isparta
37°47′N, 30°59′E
56
3
—
72
68
Sm
Sm
5
N. xanthodon
Gülek/Mersin
37°15′N, 34°45′E
56
3
—
72
68
Sm
AFigure 1
Collecting sites ofNannospalax leucodon (▲) and N. xanthodon (■) in Turkey. The numbering of sampling localities corresponds to data in Table 1.Standard voucher specimens (skins and skulls) are deposited at Selçuk University, Biology Department, Faculty of Science, Konya, Turkey.Karyotype preparations were obtained in the field from bone marrow after colchicine treatment [24]. Air-dried preparations were stained conventionally by Giemsa. Constitutive heterochromatin and nucleolus organizer regions (NORs) were detected by the techniques of C-banding [25] and silver staining of nucleolar organizer regions [26], respectively. From each specimen, 10 to 20 slides were prepared, and at least 20 well-spread metaphase plates were analysed. The system of classification of chromosomes according to the centromere position was adopted after Hsu and Benirschke [27], and the biarmed (metacentric, M, submetacentric, SM, subtelocentric, ST) and uniarmed (acrocentric, A) chromosomes were distinguished. The fundamental number of autosomal arms (NFa) and the number of all chromosomal arms in the female complement (NF) were calculated.  We followed the arrangement of chromosomes in the karyotype applied in some previous papers [17, 18, 20]. The two large acrocentric autosomal pairs which can be reliably recognized were arranged as the first and second pairs in the complement. The other biarmed and acrocentric autosomes were arranged according to their size, respectively.The distribution of the C-positive bands and AgNOR sites on individual chromosomes was summarized in the presence/absence matrix and a Neighbour Joining Clustering analysis was performed based on the character dataset using the PAST  programme [28].


## 3. Results

### 3.1. Thrace, Kırklareli Province, Babaeski Population

The karyotype of a male and a female consisted of 56 chromosomes including a single large acrocentric (no. 1), two large subtelocentric (2, 3), seven medium-sized submetacentric or subtelocentric (4–10), and 16 acrocentric autosomal pairs of gradually diminishing size (11–27) (NFa = 72). The X chromosome was a small metacentric, and the Y chromosome a small acrocentric (NF = 76) (Figure2(1)). The dark C-bands were observed in pericentromeric areas of all the biarmed and most of the acrocentric autosomes. Three acrocentric autosomal pairs were C-negatively stained (nos. 19, 26, and 27). Slight telomeric dark C-bands were observed in the short arms of the three largest biarmed autosomal pairs (2, 3, and 4).  The X chromosome had a centromeric C-positive area and the Y chromosome possessed a distinct dark pericentromeric C-positive band extending over the proxima third of the chromosome (Figure 3(1)). The NORs were observed in the telomeric regions of the short arms of the autosomes 2, 3, 4, 6, and 7. In some cells, only one homologue of the pairs 4 and 6 bore the positive silver signal (Figure 4(1)).Figure 2
Standard karyotypes of specimens ofNannospalax leucodon from Babaeski (1) and N. xanthodon from Kula (2), Alaşehir (3), Yılanlı (4), and Gülek (5).Figure 3
C-banded karyotypes of specimens ofNannospalax leucodon from Babaeski (1) and N. xanthodon from Kula (2), Alaşehir (3), Yılanlı (4), and Gülek (5).Figure 4
Silver stained karyotypes of specimens ofNannospalax leucodon from Babaeski (1) and N. xanthodon from Kula (2), Alaşehir (3), Yılanlı (4), and Gülek (5).


### 3.2. Anatolia, Manisa Province, Kula Population

The karyotype of the two males consisted of 56 chromosomes including a large acrocentric (no. 1), a large and a medium-sized metacentric (2, 3), five medium-sized submetacentric or subtelocentric (4–8), and 19 acrocentric autosomal pairs of gradually diminishing size (nos. 9–27) (NFa = 68). The X chromosome was a large submetacentric, and the Y a small acrocentric (NF = 72) (Figure2(2)). The dark C-bands were observed in pericentromeric areas of five biarmed (4–8) and four acrocentric autosomes (9, 19, 25, and 26). The X and Y chromosomes had distinct centromeric C-positive bands (Figure 3(2)). The NORs were observed in the telomeric regions of the short arms of the autosomes nos. 4, 5, and 7. In the pairs 5 and 7 the positive signal was observed in only one homologue of the pair in some cells (Figure 4(2)).


### 3.3. Anatolia, Manisa Province, Alaşehir Population

The karyotype of the five specimens examined consisted of 56 chromosomes including two large acrocentric (nos. 1, 2), two large metacentric (3, 4), six medium-sized to small submetacentric or subtelocentric (5–10), and 17 acrocentric autosomal pairs  of gradually diminishing size (11–27) (NFa = 70). The X chromosome was a large submetacentric, and the Y chromosome was a small acrocentric (NF = 74) (Figure2(3)). The dark C-bands were observed in the pericentromeric areas of five biarmed (5–7, 9, and 10) and four acrocentric autosomes (13, 19, 22, 25). The sex chromosomes had dark centromeric C-positive bands (Figure 3(3)). The NORs were observed in the telomeric regions of the short arms of the autosomes 6, 7, and 8, with an occasionally heterozygous signal on the autosome 8 (Figure 4(3)).


### 3.4. Anatolia, Isparta Province, Yılanlı Population

The karyotype of the three males consisted of 56 chromosomes including a large acrocentric (no. 1), a large submetacentric and a large metacentric (2, 3), five submetacentric or subtelocentric (4–8), and 19 acrocentric autosomal pairs of gradually diminishing size (9–27) (NFa = 68). The X and Y chromosomes were small submetacentrics (NF = 72) (Figure2(4)). The dark C-bands were observed in the pericentromeric areas of seven biarmed (2–8) and four acrocentric autosomes (10, 12, 15, and 22). The X chromosome has a distinct pericentromeric C-positive band and the Y chromosome has a slight centromeric C-positive band (Figure 3(4)). The NORs were observed in the telomeric regions of the short arms of the autosomes  4–7, with an occasionally heterozygous signal on the autosomes 5, 6, and 7 (Figure 4(4)).


### 3.5. Anatolia, Mersin Province, Gülek Population

The karyotype of the three males consisted of 56 chromosomes including two large acrocentric (nos. 1, 2), a metacentric (4), six submetacentric or subtelocentric (3, 5–9), and 18 acrocentric autosomal pairs of gradually diminishing size (10–27) (NFa = 68). The X chromosome was a medium-sized submetacentric, and the Y chromosome a small acrocentric (NF = 72) (Figure2(5)). The dark C-bands were observed in the pericentromeric areas of seven biarmed (3–9) and a single acrocentric autosomal pair (18). The X chromosome had a pericentromeric C-positive band and the Y chromosome was entirely C-negative (Figure 3(5)). The NORs were observed in the telomeric regions of the short arms of the subtelocentric autosomes nos. 3 and 8 (Figure 4(5)).


### 3.6. Karyotypic Relationships between the Populations

The distribution pattern of the C-positive band and the AgNOR sites in individual chromosomes is summarized in Tables2 and 3. The resulting neighbour-joining tree derived from the presence or absence of the characters is shown in Figure 5. The Thrace individuals of N. leucodon appeared as the basal branch which was sister to the populations of N. xanthodonfrom Anatolia.Table 2
The character distribution of the C-positive bands on individual chromosome pairs according to the presence (1) and absence (0). For population numbers see Table1.
Species/cytotypes
Chromosome no.
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
X
Y
N. leucodon/Babaeski
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
0
1
1
1
1
1
1
0
0
1
1
N. xanthodon/Kula
0
0
0
1
1
1
1
1
1
0
0
0
0
0
0
0
0
0
1
0
0
0
0
0
1
1
0
1
1
N. xanthodon/Alaşehir
0
0
0
0
1
1
1
0
1
1
0
0
1
0
0
0
0
0
1
0
0
1
0
0
1
0
0
1
1
N. xanthodon/Yılanlı
0
1
1
1
1
1
1
1
0
1
1
1
0
0
1
0
0
0
0
0
0
1
0
0
0
0
0
1
1
N. xanthodon/Gülek
0
0
1
1
1
1
1
1
1
0
0
0
0
0
0
0
0
1
0
1
0
0
0
0
0
0
0
1
0Table 3
The character distribution of the AgNOR sites on individual chromosome pairs according to the presence (1) and absence (0). For population numbers see Table1.
Species/cytotypes
Chromosome no.
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
X
Y
N. leucodon/Babaeski
0
1
1
1
0
1
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
N. xanthodon/Kula
0
0
0
1
1
0
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
N. xanthodon/Alaşehir
0
0
0
0
0
1
1
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
N. xanthodon/Yılanlı
0
0
0
1
1
1
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
N. xanthodon/Gülek
0
0
1
0
0
0
0
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0Figure 5
The neighbour-joining tree of the relationships among the studied populations based on the distribution of the C-positive bands and AgNOR sites on individual chromosome pairs.


## 3.1. Thrace, Kırklareli Province, Babaeski Population

The karyotype of a male and a female consisted of 56 chromosomes including a single large acrocentric (no. 1), two large subtelocentric (2, 3), seven medium-sized submetacentric or subtelocentric (4–10), and 16 acrocentric autosomal pairs of gradually diminishing size (11–27) (NFa = 72). The X chromosome was a small metacentric, and the Y chromosome a small acrocentric (NF = 76) (Figure2(1)). The dark C-bands were observed in pericentromeric areas of all the biarmed and most of the acrocentric autosomes. Three acrocentric autosomal pairs were C-negatively stained (nos. 19, 26, and 27). Slight telomeric dark C-bands were observed in the short arms of the three largest biarmed autosomal pairs (2, 3, and 4).  The X chromosome had a centromeric C-positive area and the Y chromosome possessed a distinct dark pericentromeric C-positive band extending over the proxima third of the chromosome (Figure 3(1)). The NORs were observed in the telomeric regions of the short arms of the autosomes 2, 3, 4, 6, and 7. In some cells, only one homologue of the pairs 4 and 6 bore the positive silver signal (Figure 4(1)).Figure 2
Standard karyotypes of specimens ofNannospalax leucodon from Babaeski (1) and N. xanthodon from Kula (2), Alaşehir (3), Yılanlı (4), and Gülek (5).Figure 3
C-banded karyotypes of specimens ofNannospalax leucodon from Babaeski (1) and N. xanthodon from Kula (2), Alaşehir (3), Yılanlı (4), and Gülek (5).Figure 4
Silver stained karyotypes of specimens ofNannospalax leucodon from Babaeski (1) and N. xanthodon from Kula (2), Alaşehir (3), Yılanlı (4), and Gülek (5).


## 3.2. Anatolia, Manisa Province, Kula Population

The karyotype of the two males consisted of 56 chromosomes including a large acrocentric (no. 1), a large and a medium-sized metacentric (2, 3), five medium-sized submetacentric or subtelocentric (4–8), and 19 acrocentric autosomal pairs of gradually diminishing size (nos. 9–27) (NFa = 68). The X chromosome was a large submetacentric, and the Y a small acrocentric (NF = 72) (Figure2(2)). The dark C-bands were observed in pericentromeric areas of five biarmed (4–8) and four acrocentric autosomes (9, 19, 25, and 26). The X and Y chromosomes had distinct centromeric C-positive bands (Figure 3(2)). The NORs were observed in the telomeric regions of the short arms of the autosomes nos. 4, 5, and 7. In the pairs 5 and 7 the positive signal was observed in only one homologue of the pair in some cells (Figure 4(2)).


## 3.3. Anatolia, Manisa Province, Alaşehir Population

The karyotype of the five specimens examined consisted of 56 chromosomes including two large acrocentric (nos. 1, 2), two large metacentric (3, 4), six medium-sized to small submetacentric or subtelocentric (5–10), and 17 acrocentric autosomal pairs  of gradually diminishing size (11–27) (NFa = 70). The X chromosome was a large submetacentric, and the Y chromosome was a small acrocentric (NF = 74) (Figure2(3)). The dark C-bands were observed in the pericentromeric areas of five biarmed (5–7, 9, and 10) and four acrocentric autosomes (13, 19, 22, 25). The sex chromosomes had dark centromeric C-positive bands (Figure 3(3)). The NORs were observed in the telomeric regions of the short arms of the autosomes 6, 7, and 8, with an occasionally heterozygous signal on the autosome 8 (Figure 4(3)).


## 3.4. Anatolia, Isparta Province, Yılanlı Population

The karyotype of the three males consisted of 56 chromosomes including a large acrocentric (no. 1), a large submetacentric and a large metacentric (2, 3), five submetacentric or subtelocentric (4–8), and 19 acrocentric autosomal pairs of gradually diminishing size (9–27) (NFa = 68). The X and Y chromosomes were small submetacentrics (NF = 72) (Figure2(4)). The dark C-bands were observed in the pericentromeric areas of seven biarmed (2–8) and four acrocentric autosomes (10, 12, 15, and 22). The X chromosome has a distinct pericentromeric C-positive band and the Y chromosome has a slight centromeric C-positive band (Figure 3(4)). The NORs were observed in the telomeric regions of the short arms of the autosomes  4–7, with an occasionally heterozygous signal on the autosomes 5, 6, and 7 (Figure 4(4)).


## 3.5. Anatolia, Mersin Province, Gülek Population

The karyotype of the three males consisted of 56 chromosomes including two large acrocentric (nos. 1, 2), a metacentric (4), six submetacentric or subtelocentric (3, 5–9), and 18 acrocentric autosomal pairs of gradually diminishing size (10–27) (NFa = 68). The X chromosome was a medium-sized submetacentric, and the Y chromosome a small acrocentric (NF = 72) (Figure2(5)). The dark C-bands were observed in the pericentromeric areas of seven biarmed (3–9) and a single acrocentric autosomal pair (18). The X chromosome had a pericentromeric C-positive band and the Y chromosome was entirely C-negative (Figure 3(5)). The NORs were observed in the telomeric regions of the short arms of the subtelocentric autosomes nos. 3 and 8 (Figure 4(5)).


## 3.6. Karyotypic Relationships between the Populations

The distribution pattern of the C-positive band and the AgNOR sites in individual chromosomes is summarized in Tables2 and 3. The resulting neighbour-joining tree derived from the presence or absence of the characters is shown in Figure 5. The Thrace individuals of N. leucodon appeared as the basal branch which was sister to the populations of N. xanthodonfrom Anatolia.Table 2
The character distribution of the C-positive bands on individual chromosome pairs according to the presence (1) and absence (0). For population numbers see Table1.
Species/cytotypes
Chromosome no.
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
X
Y
N. leucodon/Babaeski
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
0
1
1
1
1
1
1
0
0
1
1
N. xanthodon/Kula
0
0
0
1
1
1
1
1
1
0
0
0
0
0
0
0
0
0
1
0
0
0
0
0
1
1
0
1
1
N. xanthodon/Alaşehir
0
0
0
0
1
1
1
0
1
1
0
0
1
0
0
0
0
0
1
0
0
1
0
0
1
0
0
1
1
N. xanthodon/Yılanlı
0
1
1
1
1
1
1
1
0
1
1
1
0
0
1
0
0
0
0
0
0
1
0
0
0
0
0
1
1
N. xanthodon/Gülek
0
0
1
1
1
1
1
1
1
0
0
0
0
0
0
0
0
1
0
1
0
0
0
0
0
0
0
1
0Table 3
The character distribution of the AgNOR sites on individual chromosome pairs according to the presence (1) and absence (0). For population numbers see Table1.
Species/cytotypes
Chromosome no.
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
X
Y
N. leucodon/Babaeski
0
1
1
1
0
1
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
N. xanthodon/Kula
0
0
0
1
1
0
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
N. xanthodon/Alaşehir
0
0
0
0
0
1
1
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
N. xanthodon/Yılanlı
0
0
0
1
1
1
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
N. xanthodon/Gülek
0
0
1
0
0
0
0
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0Figure 5
The neighbour-joining tree of the relationships among the studied populations based on the distribution of the C-positive bands and AgNOR sites on individual chromosome pairs.


## 4. Discussion

The samples of specimens from each of the populations studied revealed a distinct karyotype which was different from those found in other populations. At the same time, no variation between specimens originating from a single population was recorded, except for the occasionally heterozygous NOR signal observed in some autosomal pairs. The karyotypes found seem identical or similar to those reported by conventional staining in mole rats from the same areas (e.g., [10, 12–16]).It is difficult to assess exactly the nature of chromosomal variation found between populations without the use of structural banding or chromosome painting. Nevertheless, all the karyotypes examined contained the same number of chromosomes and this uniformity indicates that the Robertsonian changes, proposed as a main mechanism of karyotype divergence in mole rats [13], could hardly participate extensively in the process of chromosomal differentiation among the studied populations. The overall extent of observed variation between the 2n=56 karyotypes from individual geographic populations is rather wide and indicates considerable chromosomal differentiation resulting from varied rearrangements. The role of C-heterochromatin changes and centromeric shifts and inversions can be assumed. The karyotypes with the same diploid number may apparently have evolved separately as a consequence of convergent events.From the phenetic point of view which can be derived from the banding patterns obtained, the most distinct complement is that of specimens from Thrace. The karyotypes of other populations with 56 chromosomes studied in Anatolia differ from that found in the Thrace individuals in several aspects. The karyotype of the specimens from Thrace includes two large subtelocentric autosomal pairs that obviously do not occur in complements of specimens from the other populations. The large subtelocentric autosomes with the active NOR sites at the short arms were found quite exceptionally in other cytotypes classified asN. xanthodon in Anatolia. Similar autosomal pairs were reported only in the 2n=36 and 38 cytotypes from western Anatolia but the short arms were completely C-heterochromatic in the 2n=38 karyotype [22]. On the other hand, similar large subtelocentric autosomal pairs were frequently recorded in karyotypes of the populations classified as N. leucodon from southeastern Europe (e.g., [29, 30]).The number of biarmed autosomes and, consequently, the number of autosomal arms are the highest in the Thrace sample, and also the amount of C-heterochromatin is distinctly larger in this population. In the Anatolian populations, the amount of C-heterochromatin is generally lower, particularly in specimens from the easternmost site in Gülek. The low amount of C-heterochromatin has also been reported in other studied cytotypes ofN. xanthodon[19–22]. Three large subtelocentric autosomal pairs from the Thrace karyotype possess slight dark C-bands localized in the telomeric region of the short arms and similar C-positive telomeric bands were not recorded in any karyotype of specimens of the Anatolian origin.The active NOR sites were found on five autosomal pairs in specimens from Thrace and this number was higher than in the other2n=56 karyotypes where two, three, or four NOR bearing pairs were observed. The lower number of NORs not exceeding three of four pairs was recorded in various cytotypes of N. xanthodon in Anatolia (e.g., [19–22, 31]). However, the active NOR sites were recorded in five pairs of subtelocentric autosomes in various populations of the 2n=60 cytotype of N. xanthodon from Anatolia [18].The small size of the X chromosome in the karyotype of specimens from Thrace is quite exceptional among the populations of mole rats studied so far. The X chromosome belonged among the smallest elements in the complement and its relative length was distinctly lower than 6-7% of the haploid set, which is the value usually reported in mammals [32]. The small size of the X chromosome in the Thrace individuals is quite unique. Such a small X chromosome has not been reported from any other N. leucodon population in southeastern Europe (e.g., [30]) and it is unknown also in karyotypes of populations belonging to other mole rat species. Moderate variation in the size of the X was recorded also in the 56-chromosome complements of  samples studied in Anatolia. The size of the X chromosome in specimens from Yılanlı is only slightly larger than that recorded in the Thrace sample. The X chromosome of the specimens originating Gülek is medium-sized, whereas the X chromosomes of the specimens from Kula and Alaşehir were relatively large.The large acrocentric autosomes are represented by one or two pairs in the complements of the studied populations. We have not recorded additions of heterochromatic short arms on this chromosome reported in other populations ofN. xanthodon (e.g., [20]). Surprisingly, we have not found any whole-heterochromatin short arms in the karyotypes examined. The complements of specimens from Kula and Alaşehir in western Anatolia contain two pairs of distinctly large metacentric autosomes. Similar autosomal pairs were found also in the karyotype of the specimens from Yılanlı but the centromere position in the larger pair was submetacentric.The differences between the studied populations were quantified in the analysis of the distribution pattern of the C-positive bands and the AgNOR sites which indicated a possible divergence pattern with the Thrace individuals ofN. leucodon appearing as a basal branch of the tree. We are aware that this analysis is only preliminary because of the tentative identification of the individual autosomal pairs; however, the results are congruent with the overall phenetic assessment of the observed chromosomal variation as well as with the results of recent studies of molecular phylogeny [33–35]. The Thrace population, classified as N. leucodon, actually appears as a sister group of the Anatolian populations with the same diploid number recognized as N. xanthodon.


## 5. Conclusions

We can conclude that the karyotypes of the 56-chromosome populations ofN. leucodon and N. xanthodon are distinctly different from each other but it is difficult to relate directly this difference to considerations of the species status because of the overall extensive karyotypic variation found within the species and the genus. The reproductive isolation, the occurrence of gene flow, and the genetic distance between presumptive species of mole rats should be studied in this respect with the use of molecular approach. Further genetic studies focused on appropriate molecular markers are thus needed to elucidate the problem.



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