Newsgroup: it.scienza.medicina
Titolo: mucca pazza info

scusate la domanda, sono un "estraneo ai lavori": esiste un test per sapere
se si è infettati ? Esiste un periodo finestra ?

 se si è infettati ? Esiste un periodo finestra ?
Che io sappia, non esiste test, al riguardo.
Del resto, la diagnosi ragionevolmente certa la si puo' fare solo
analizzando, al momento, il cervello del paziente, che deve quindi essere
gia' deceduto.
Saluti.
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Da #misteri:
 - perchè son sempre incazzati i "paranormalisti" ?
 - gli cascano i tavolini sui piedi
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                                 ^^SpAwN^^

 > se si č infettati ? Esiste un periodo finestra ?
- da un sito inglese:
Cerebrospinal Fluid
Examination of the cerebrospinal fluid (CSF) in patients with CJD is
sometimes abnormal. Extrapolating from Will and Matthews' data, the
CSF protein was greater than 0.4 grammes per litre in 45% of cases
where it was examined. The protein content rarely exceeds 1.0 gramme
per litre (although one report describes a case with a protein of
5g/l), and a leucocyte response is absent. Three cases with
oligoclonal bands in the CSF have been reported. Of recent interest is
the presence in the CSF of neuron-specific enolase identified by
two-dimensional electrophoresis. The presence of two CSF proteins,
p130 and p131 (which are not prion protein) is claimed to have high
sensitivity and specificity for CJD by a group of scientists from the
National Institute of Health in North America. We are currently
evaluating this test with CSF from suspect cases of CJD in the UK.
Electroencephalography
At present the EEG is the most useful diagnostic test for CJD and in
our experience 1-2 cycle/sec generalised triphasic periodic sharp wave
complexes in the appropriate clinical setting is virtually diagnostic
of CJD. Approximately 60% of our cases had this characteristic
appearance during their illness. The high figure from the previous
studies is perhaps a reflection of the number of EEGs performed in
their cases. Also, given the subjective nature of EEG interpretation,
there may have been some variation in the types of abnormalities
included as characteristic. There is no clear definition of a
`typical' EEG but we have a high threshold for classifying features as
typical. However it should be bourne in mind that the EEG frequently
does not show the characteristic pattern even if performed often up to
the time of death. Additionally, unilateral rather than generalised
periodic complexes occasionally occur in definite cases. The EEG
appearance can be suppressed by the administration of benzodiazepines
and also be produced following its administration. Lack of the typical
EEG appearance is associated with long clinical duration and disease
aetiology; the classical appearance is not seen in the iatrogenic
growth hormone related cases or kuru. Anecdotal reports of false
positive diagnoses have usually arisen from the incorrect assessment
of EEGs, and the inaccurate description of non-specific EEG findings
as "characteristic" of CJD. The differential diagnosis of generalised
triphasic periodic complexes is given below.
- inoltre test Western blot su CSF:
Laboratory diagnosis of variant Creutzfeldt-Jakob disease.
Ironside JW, Head MW, Bell JE, McCardle L, Will RG
Departments of Pathology, Clinical Neurosciences, CJD Surveillance
Unit, University of Edinburgh, Western General Hospital, Edinburgh,
UK. 
J.W.Ir...@ed.ac.uk
The neuropathological and biochemical features of 33 cases of variant
Creutzfeldt-Jakob disease (vCJD) diagnosed up to the end of 1998 are
analysed in relation to the 646 cases of suspected CJD referred to the
CJD Surveillance Unit laboratory from 1990 to 1998. Morphological
studies of the central nervous system, lymphoid tissues and other
organs were accompanied by immunocytochemistry; Western blot analysis
of PrPRES was performed on frozen brain tissue. The findings were
analysed in relation to clinical and genetic data. The pathology of
vCJD showed morphological and immunocytochemical characteristics
distinct from other cases of CJD. PrP accumulation was widespread in
lymphoid tissues in vCJD, but was not identified in other non-neural
tissues. PrPRES accumulation in vCJD brain tissue showed a uniform
glycotype pattern distinct from sporadic CJD. All analysed cases of
vCJD were methionine homozygotes at codon 129 of the PrP gene. No
evidence currently exists to suggest that cases of CJD diagnosed in
individuals who are MV or VV at codon 129 of the PrP gene represent
'human bovine spongiform encaphalopathy (BSE)'. Continued surveillance
is required to further investigate this possibility, with the need to
investigate autopsy tissues from suspected cases by histological and
biochemical techniques.
-infine ho trovato un metodo in citofluorimetria che sembra essere il
piů sensibile (sempre da CSF) ma ancora in fase di sviluppo.
Davide Dr. Stangalini
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www.fleming-research.it