Olfactory receptors on the maxillary palps of small ermine moth larvae : evolutionary history of benzaldehyde sensitivity In lepidopterous larvae the maxillary palps contain a large portion of the sensory equipment of the insect . Yet , knowledge about the sensitivity of these cells is limited . In this paper a morphological , behavioral , and electrophysiological investigation of the maxillary palps of Yponomeuta cagnagellus -LRB- Lepidoptera : Yponomeutidae -RRB- is presented . In addition to thermoreceptors , CO2 receptors , and gustatory receptors , evidence is reported for the existence of two groups of receptor cells sensitive to plant volatiles . Cells that are mainly sensitive to -LRB- E -RRB- -2-hexenal and hexanal or to -LRB- Z -RRB- -3-hexen-1-ol and 1-hexanol were found . Interestingly , a high sensitivity for benzaldehyde was also found . This compound is not known to be present in Euonymus europaeus , the host plant of the monophagous Yponomeuta cagnagellus , but it is a prominent compound in Rosaceae , the presumed hosts of the ancestors of Y. cagnagellus . To elucidate the evolutionary history of this sensitivity , and its possible role in host shifts , feeding responses of three Yponomeuta species to benzaldehyde were investigated . The results confirm the hypothesis that the sensitivity to benzaldehyde evolved during the ancestral shift from Celastraceae to Rosaceae and can be considered an evolutionary relict , retained in the recently backshifted Celastraceae-specialist Y. cagnagellus . Introduction Recently , progress has been made in our understanding of speciation in herbivorous insects , and mathematical models demonstrate that adaptive speciation driven by selection on ecological traits is theoretically plausible -LRB- Kondrashov and Kondrashov 1999 ; Dickman and Doebeli 1999 ; Doebeli 2005 -RRB- . However , as yet not much work has been done on the actual nature of these traits . The theoretical results make clear that host shifts and concomitant changes in host acceptance behaviour are important factors in adaptive speciation , but the underlying changes in the neural system are poorly studied and understood . Only recently work addressing this type of question is starting to appear -LRB- cf. Olsson et al. 2005a , b -RRB- . Although host acceptance in phytophagous insects is usually viewed as solely governed by the adult -LRB- `` mother knows best '' -RRB- , it should be noted that in many species larval host acceptance is equally important . Lepidopterous larvae in particular often display striking food preferences , which are based on a small set of chemoreceptors -LRB- Schoonhoven and van Loon 2002 -RRB- , thus making caterpillars promising models to study the neural basis of host plant recognition , host shifts , and the evolution of insect -- plant relations . However , such an approach requires detailed knowledge about the responses of the involved receptors , information that is almost completely lacking . External chemoreceptors in caterpillars are located on the antennae , on the maxillae , and in the epipharynx . The gustatory sensilla styloconica on the galea are important in host acceptance behaviour , and a large body of data is available about their sensitivity -LRB- see Schoonhoven and van Loon 2002 ; Schoonhoven et al. 2005 for review -RRB- . The other sets of receptors , the epipharyngeal organs on the inside of the labrum , and the sensilla on the antennae and the maxillary palps , are more difficult to study and as a consequence less well known . The maxillary palp receptors in particular have received very little attention . Except for the pioneering studies of Schoonhoven and Dethier -LRB- 1966 -RRB- , recordings from the maxillary nerve of the silkworm by Hirao et al. -LRB- 1976 , and a recent paper on gustatory receptors in the palp of Choristoneura fumiferana -LRB- Albert 2003 -RRB- , no electrophysiological data on the maxillary palps of Lepidoptera appears to be available . However , behavioural work indicates that the influence of the palps on feeding can be as significant as that of the taste sensilla on the galea -LRB- Hanson and Dethier 1973 ; Glendinning et al. 1998 ; de Boer 1993 , 2006 -RRB- . The small ermine moth genus Yponomeuta Latreille -LRB- Lepidoptera : Yponomeutidae -RRB- consists of some 75 species -LRB- Gershenson and Ulenberg 1998 -RRB- . Most are specialised on Celastraceae , and phylogenetic analysis -LRB- Menken 1996 ; Turner H , Lieshout N , van Ginkel W , Menken SBJ , submitted ; Ulenberg , personal communication -RRB- strongly suggest that this association is the ancestral state in the genus . Moreover , mapping host use upon the phylogeny indicates an intriguing evolutionary scenario : the genus most likely originated in the Far East and dispersed to the western Palearctic concomitant with a -LRB- probably single -RRB- shift to Rosaceae and further to Salicaceae -LRB- Menken 1996 -RRB- . Interestingly , one lineage within this clade , Y. cagnagellus -LRB- Hübner -RRB- , shifted back to the ancestral host , so this species is secondarily associated with Celastraceae . Yponomeuta plumbellus on the other hand is present in Western Europe , but this species is more closely related to the clades in the Far East , and retained the ancestral relation with Celastraceae . The feeding of the Western European Yponomeuta species , and in particular Y. cagnagellus , is relatively well studied . Both the behaviour -LRB- Gerrits-Heybroek et al. 1978 ; van Drongelen 1980 ; Kooi and van de Water 1988 ; Peterson et al. 1990 -RRB- and the underlying sensory physiology -LRB- van Drongelen 1979 ; Roessingh et al. 1999 -RRB- have been addressed . The results demonstrate a clade of predominantly monophagous species with well-adapted sensory systems displaying high sensitivity for sugar alcohols characteristic for the host -- plant families , as well as reduced sensitivities for host-specific deterrents . However , this whole body of work is focused on gustatory responses of styloconic sensilla on the galea that may provide only part of the relevant sensory inputs -LRB- de Boer 2006 -RRB- . Here we present a morphological , behavioural , and electrophysiological survey of chemoreceptors on the maxillary palps in the larvae of Yponomeuta cagnagellus . In addition , we performed a comparative study into the effects of benzaldehyde on feeding behaviour of three related Yponomeuta species . Benzaldehyde strongly stimulated sensory cells in the maxillary palp of Y. cagnagellus , but its role in feeding behaviour is unknown . This compound is of particular interest because it is prominent in Rosaceae such as Prunus spinosa -LRB- Humpf and Schreier 1992 -RRB- , the host of Y. padellus -LRB- L -RRB- , but absent from Euonymus europaeus , the host of Y. cagnagellus and Y. plumbellus -LRB- Denis and Schiffermüller -RRB- -LRB- Fig. 1 -RRB- . In this comparison we take advantage of the fact that the phylogenetic tree indicates that Y. plumbellus , although present in Western Europe , belongs to a clade that still displays the original ancestral association with Celastraceae . Yponomeuta padellus is in the middle of the clade that shifted to the benzaldehyde containing Rosaceae . Yponomeuta cagnagellus on the other hand is now feeding on the Celastraceous E. europaeus , that does not contain benzaldehyde , but this species most likely originated from an ancestor that was feeding on Rosaceae . An analysis of the phylogenetic pattern of the response to benzaldehyde in these three species will therefore contribute to our understanding of the flexibility of sensory systems and the role of sensory stimuli in host shifts and adaptive speciation . Fig. 1Phylogeny of Yponomeuta , based on the internal transcribed spacer region -LRB- ITS-1 -RRB- and on 16S rDNA -LRB- 16S -RRB- and cytochrome oxidase -LRB- COII -RRB- mitochondrial genes , using maximum parsimony -LRB- Turner H , Lieshout N , van Ginkel W , Menken SBJ , submitted -RRB- . Host plant use is plotted on the tree and the presence of benzaldehyde in the host and feeding responses to this compound are indicated for the three tested species Materials and methods Insects Egg batches of Y. cagnagellus and Y. padellus were collected in the field in The Netherlands from their host plants : Y. cagnagellus from E. europaeus and Y. padellus from P. spinosa and Crataegus spp. . Yponomeuta plumbellus larvae were collected as fourth instars . Egg batches were kept at 4 °C and 80 % relative humidity until needed . Larvae were reared in 10 cm wide Petri dishes -LRB- at 25 °C , ambient humidity , L18 : D6 h photoperiod -RRB- on host foliage from potted plants grown in the greenhouse -LRB- E. europaeus for Y. cagnagellus and Y. plumbellus ; P. spinosa for Y. padellus -RRB- . During the rearing period larvae were fed ad libitum . For the electrophysiological experiments . 1 - to 4-day-old fifth instars were used that were starved for 2 -- 3 h . In feeding experiments the caterpillars were used during the whole duration of the fifth stadium . Between feeding experiments , caterpillars were kept at 4 °C . Morphology The morphology of the palps was studied using scanning EM . Fixation and drying often induce shrinking and cause collapse of soft tissue parts . We avoided these problems by using Cryo-SEM . Larvae were used directly after moulting into the fifth stadium . The preparation was stabilized by quick immersion in nitrogen slush -LRB- 60 K -RRB- , coated with a 200 Å thick layer of gold and viewed in the frozen state using a Philips 535 SEM equipped with a Hexland CT1000/CP2000 and a Jeol 35C SEM equipped with an EMSCOPE SP2000A cryo preparation assembly . Host plant odour composition One of the problems in determining the function of unknown sensory organs is the choice of the stimuli to be tested . To identify a set of relevant substances , the chemical composition of the headspace of E. europaeus , the host of Y. cagnagellus , was characterized . Branches -LRB- 30 cm long -RRB- of E. europaeus growing in the field in Wageningen , the Netherlands , were cut with sharp garden scissors and transferred to the lab . About 2 kg of branches was placed in a 20-l stainless steel vessel within 30 min after cutting . Care was taken not to damage the leaves . With a membrane pump air was passed through KOH pellets , molecular sieves 5A and 3 × , active charcoal , and a tenax pre-trap . This cleaned air flowed for 7 -- 15 h at a rate of 70 ml/min through the stainless steel vessel and subsequently through the tenax-TA sample trap . The collected volatiles were released from the tenax by the TCT technique -LRB- Thermo-desorption cold trap ; Dicke et al. 1990 -RRB- . The TCT unit -LRB- Chrompack , Middelburg , The Netherlands -RRB- was heated -LRB- 250 °C -RRB- for 3 min and desorbed compounds were collected on a Sil5CB coated , fused silica capillary at − 100 °C . Flash heating of the cold trap provided sharp injection of the compounds into the GC/MS unit -LRB- Chrompack Sil19CB column , 25 m × 0.25 mm , df 0.25 μm -RRB- . Electron impact ionisation was carried out at 70 eV on a VG MM 7070F mass spectrometer . Electrophysiological recording and odour stimuli A diagram of the recording set-up is presented in Fig. 2 . An isolated caterpillar head was mounted on a silver wire loop and connected via a 10-mm connector to the input terminal of a laboratory-built amplifier -LRB- Ri > 109 Ω , Ci < 1 pF , Ib < 1 pA -RRB- . The sensory cells of the sensilla on the maxillary palp are located below the base of the terminal segment -LRB- Schoonhoven and Dethier 1966 ; Albert 1980 -RRB- . To gain access to these cells , the connecting membrane between the two most distal segments was pierced with a sharpened tungsten electrode . The actual single cell recordings were made using a glass capillary electrode filled with 3 M KCl -LRB- impedance about 40 MΩ -RRB- inserted through the hole and connected to ground -LRB- so actually serving as the indifferent electrode -RRB- . The position of the glass electrode was carefully adjusted until action potentials could be recorded . The inside of the palp is probed with the tip until activity is picked up , analogous to the way in which recordings from the CNS are made . It should be noted that although this procedure has the advantage of allowing the recoding from single cells with good S/N ratios , and easy identification of separate cells , the drawback is that the position of the tip -LRB- and therefore the identity of the recorded cell -RRB- is not known , and not reproducible between recordings . Fig. 2Schematic diagram of the electrophysiological set-up used to measure responses to both gustatory and olfactory stimuli . Open arrows indicate airflow , black arrows electrical signals . Note that in contrast to standard tip recording the stimulus pipette is electrically isolated The preparation was placed in a continuous stream of clean , moistened air -LRB- 80 ml/s , 25 cm/s -RRB- . A second -LRB- control -RRB- air stream -LRB- 2 ml/s -RRB- was injected in the main stream through an empty pasteur pipette . Odour stimuli were delivered by switching this second stream for 1 s through a pasteur pipette containing the stimulus . For stimulation with CO2 , a single injected stream of commercial available bottled CO2 -LRB- Hoekloos , the Netherlands -RRB- was switched on . This injected stream could be adjusted to produce CO2 concentrations in the main stream between 0.1 and 10 % . Olfactory stimuli were diluted in paraffin oil -LRB- 10 − 3 , 10 − 2 , and 10 − 1 v/v -RRB- . Twenty-five microlitres of these solutions was applied to filter paper strips -LRB- 6.0 × 0.5 cm -RRB- and was placed in pasteur pipettes . The following compounds -LRB- 99 % pure -RRB- were used : 1-hexanol and benzaldehyde -LRB- Fluka , Switzerland -RRB- ; -LRB- Z -RRB- -3-hexenyl acetate and -LRB- E -RRB- -2-hexenal -LRB- Z -RRB- -3-hexen-1-ol -LRB- Roth , West Germany -RRB- ; hexanal -LRB- 98 % pure -RRB- -LRB- Merck , USA -RRB- ; limonene , citral -LRB- 97 % pure -RRB- , and geraniol -LRB- 98 % pure -RRB- -LRB- Aldrich , Germany -RRB- . All stimuli were applied in random order with at least 30 s between them . Longer intervals were used after strong stimuli . Preparations were discarded after 60 min and were changed after a successful recording . Odour stimuli were stored at 4 °C between recording sessions , and replaced every few days . The AC amplified action potentials from the sensory cells were recorded on tape -LRB- Racal FM taperecorder -RRB- . To facilitate computer-assisted analysis , the valve signal and a pre-pulse 1 s earlier were recorded on an additional channel . Analysis of recordings The recordings contained activity from one to four sensory cells but usually not more than two . Recordings with more than three cells were discarded . Multi-cellular responses were analysed with the help of an interactive computer program based on the methods described in Roessingh et al. -LRB- 1992 -RRB- . Spikes were characterized primarily by amplitude . Amplitude boundaries for different cells were taken from an amplitude versus time plot . The separation of the cells is an interactive process in which spike amplitude and spike interval distributions are used as criteria to judge the separation . After the separation , responses for each cell were calculated as the difference in action potential frequency in the last second before the onset of stimulation and the first reaction second . A cell was assumed to respond to a stimulus if an increase of at least ten spikes could be evoked . Cells with weaker reactions were assumed to be sensitive to untested stimuli and were not further analysed . To test the significance of observed differences in spike frequency , analysis of variance was used on log transformed response data . No-choice feeding assay A new no-choice feeding assay was designed to investigate the effect of benzaldehyde odour on larval feeding . The experimental setup is illustrated in Fig. 3 . A clean air stream was split into two branches , with airflow meters to control the flow . Each branch was connected to a cluster of 18 Petri dishes , each dish containing a feeding caterpillar . In the control group , clean air was passed over the feeding larvae . The air for the experimental group was passed through a small bottle of benzaldehyde diluted in paraffin oil . A bypass with a needle valve allowed mixing in clear air and fine-tuning of the concentration of benzaldehyde -LRB- i.e. 50 % flow through the bypass reduces the final concentration with a factor 2 . Six different dilutions -LRB- v/v -RRB- of benzaldehyde in paraffin oil were used in the experiments , viz. 0.5 × 10 − 4 , 0.5 × 10 − 3 , 0.2 × 10 − 2 , 0.5 × 10 − 2 , 1 × 10 − 2 , and 0.5 × 10 − 1 . The total airflow was approximately 50 ml/s , resulting in a flow of approximately 1.4 ml/s through each Petri dish . Fig. 3Feeding assay air flow diagram . Both the control group and treatment group consisted of 18 Petri dishes A leaf disc with a diameter of 11 mm made from fresh host leaves was placed in each Petri dish on top of a piece of filter paper slightly wetted with distilled water . To reduce baseline palatability and detect feeding stimulation and/or inhibition , the leaf discs were extracted in alcohol and then washed in distilled water for 10 min . The duration of extraction needed to induce a weak feeding response depending upon both the host plants and leaf quality . For Y. cagnagellus , E. europaeus leaf discs were soaked in 96 % EtOH for 24 h. Prunus spinosa leaf discs for Y. padellus were soaked for 2 h in 70 % EtOH . Yponomeuta plumbellus appears later in the season than the other two species and feeds on slightly older E. europaeus foliage . For these older leaves extraction of leaf discs for 1 h in EtOH was sufficient to reduce palatability . The feeding experiments lasted 4 -- 5 h. Usually 1 or 2 h after the start of the experiment , caterpillars started eating . The experiment was terminated after at least two third of the insects had been eating for more than 3 h. To quantify the amount of feeding , the remainders of the leaf discs were scanned with an EPSON perfection 1250 scanner . The surface area was measured on a Macintosh computer using the open source program NIH Image version 1.62 -LRB- developed at the U.S. National Institutes of Health and available on the Internet at http://www.rsb.info.nih.gov/nih-image/ -RRB- . The differences in consumption between treatment and control groups were evaluated using a Mann -- Whitney test in SPSS -LRB- SPSS Inc. 2001 -RRB- . For graphical display a feeding index -LRB- C − T -RRB- / -LRB- C + T -RRB- × 100 % -LRB- ranging from − 100 to 100 % -RRB- , was calculated , in which C and T denote , respectively , the mean of the remaining leaf surface in the control group and in the treatment group . Results Morphology The palps of Y. cagnagellus are more elongated than in most other species of Lepidoptera , however , the sensory equipment does conform to the general pattern of ditrysian species as outlined by Grimes and Neunzig -LRB- 1986 -RRB- . These authors divide the eight sensilla on the apex in a medial group -LRB- M1 , M2 -RRB- , an apical group -LRB- A1 , A2 , A3 -RRB- , and a lateral group -LRB- L1 , L2 , L3 -RRB- . These groups can also be recognised in Y. cagnagellus , although the position of the sensilla is more linear than in the generalised pattern -LRB- 4c -RRB- . On the apex seven sensilla basiconica and one sensillum styloconicum can be distinguished . On the side of the segment , a digitiform sensillum and at least one campaniform sensillum are visible -LRB- 4b -RRB- . With the exception of the styloconic sensillum , which has a prominent base , the sensilla on the apex are simple blunt cones . The lateral and medial sensilla , for which a gustatory function has been suggested , seem to be slightly more tapered -LRB- Fig. 4e , f -RRB- but the difference is only slight . Structural details like grooved surfaces or apical pores were not observed . Fig. 4Cryo-SEM micrographs of Yponomeuta cagnagellus.a Ventral view of the head . b Maxillary palp showing the digitiform sensillum -LRB- leftmost arrow -RRB- and a large campaniform sensillum -LRB- rightmost arrow -RRB- . c Overview of the tip of the most distal segment . Note the linear arrangement of the sensilla . d Detail of the digitiform sensillum . e On the apex of the palp , seven sensilla basiconica and one sensillum styloconicum are present . From left to right , L1 , L2 , L3 , A1 , a styloconic A2 , A3 , a partly hidden M1 , and M2 can be seen -LRB- terminology after Grimes and Neunzig 1986 -RRB- . f High magnification of the lateral side of the apex . The two leftmost sensilla belong to the lateral group . The slightly more blunt sensillum on the right is A1 . Note the absence of surface structures Host plant odour composition The compounds used for olfactory stimulation were chosen on the basis of the headspace analysis of E. europaeus . Whole undamaged plants release only limited amounts of volatiles -LRB- Buttery et al. 1985 ; van Poecke and Dicke 2004 -RRB- , and indeed small quantities were found in the present study . The only compounds that could be detected consistently were C6-based `` green odours '' -LRB- Visser and Avé 1978 -RRB- . Other compounds -LRB- limonene , beta-ocimene , alpha-copaene , beta-bourbonene , benzaldehyde -RRB- could not be detected reliably . Based on this result -LRB- Z -RRB- -3-hexenyl acetate -LRB- E -RRB- -2-hexenal , 1-hexanal -LRB- Z -RRB- -3-hexen-1-ol , hexanol , limonene , citral , geraniol , and benzaldehyde were chosen as olfactory stimuli . Benzaldehyde is not known from E. europaeus , but was nevertheless included in the analysis because it is a major compound in the Rosaceous hosts of some other Yponomeuta species that are closely related to Y. cagnagellus . Electrophysiology In experiments on 69 preparations , a total of 85 single cells were analysed . Twenty-four cells did not respond to any of the applied stimuli . Of the remaining receptors , 22 cells showed no response to odours , but responded to a 100 mM KCl solution . However , these gustatory responses were only weak -LRB- 21 ± 4 spikes maximum -RRB- so the adequate stimulus was most likely not KCl . Gustatory responses will therefore not be considered here any further . Twenty-two other cells responded strongly to C6 compounds and to benzaldehyde -LRB- Fig. 5 -RRB- . For 19 of those cells more or less complete response spectra could be obtained -LRB- Fig. 6 -RRB- . At least two groups could be distinguished . Cells one to five responded to the aldehydes -LRB- E -RRB- -2-hexenal and hexanal . The other cells respond to the leaf alcohol -LRB- Z -RRB- -3-hexen-1-ol , to 1-hexanol , and to benzaldehyde . No cells were found that responded strongly to -LRB- Z -RRB- -3-hexen-1-yl acetate , or to the terpenoids limonene -LRB- 6 cells tested -RRB- , citral -LRB- 3 cells tested -RRB- and geraniol -LRB- 5 cells tested -RRB- . Fig. 5I Responses of a -LRB- E -RRB- -2 hexenal cell in the maxillary palp of Yponomeuta cagnagellus to plant odour compounds . A Hexanal 10 − 1 , B Hexanal 10 − 2 . C -LRB- Z -RRB- -3-hexen-1-ol 10 − 1 . D -LRB- Z -RRB- -3-hexen-1-ol 10 − 2 . The stimulus period -LRB- 1 s -RRB- is indicated by the horizontal bar . II Responses of a -LRB- Z -RRB- -3-hexen-1-ol best cell in the maxillary palp of Yponomeuta cagnagellus to plant odour compounds . A -LRB- Z -RRB- -3-hexen-1-ol 10 − 1 . B -LRB- Z -RRB- -3-hexen-1-ol 10 − 2 , C -LRB- Z -RRB- -3-hexen-1-ol 10 − 3 . D -LRB- E -RRB- -2-hexenal 10 − 2 , E hexanal 10 − 2 , F benzaldehyde 10 − 2 . Note in D and E the presence of an aldehyde-sensitive cell in the noise -LRB- arrow -RRB- . The stimulus duration -LRB- 1 s -RRB- is indicated by the horizontal barFig . 6Response spectra for 19 cells sensitive to plant odour compounds in the maxillary palps of Yponomeuta cagnagellus at a concentration of 10 − 2 . Response intensity is indicated by the diameter of the circles . Empty spots are missing observations Dose response relations for green odours in the two main cell groups are given in Fig. 7 . In Fig. 7a , the responses of the -LRB- E -RRB- -2-hexenal sensitive cells to the other green odours are plotted . The same is done in Fig. 7b for the -LRB- Z -RRB- -3-hexen-1-ol cells . In the alcohol-sensitive group , several cells responded to both -LRB- Z -RRB- -3-hexen-1-ol and 1-hexanol -LRB- cells 6 -- 12 -RRB- but cells that reacted mainly to one of the compounds were also found -LRB- cells 13 -- 19 -RRB- . Fig. 7Dose -- response relations of olfactory receptor cells in the cells of Yponomeuta cagnagellus . a -LRB- E -RRB- -2-hexenal sensitive cells -LRB- data from cell 1 to 5 in Fig. 6 , plus three additional cells -RRB- . b -LRB- Z -RRB- -3-hexen-1-ol sensitive cells -LRB- data from cell 6 to 19 in Fig. 6 -RRB- . c. benzaldehyde sensitive cells -LRB- data from cell 6 to 16 in Fig. 6 -RRB- . The number of sensory cells actually recorded for each datapoint is given between brackets , vertical bars indicate the SE The -LRB- Z -RRB- -3-hexen-1-ol and 1-hexanol cells were often also highly sensitive to benzaldehyde -LRB- Fig. 7c -RRB- . Evidence indicating that this sensitivity could be attributed to another cell -LRB- e.g. superpositions or irregular spike interval histograms -RRB- was not observed . The `` aldehyde '' cells in our sample did not show this sensitivity and only two cells responded weakly to benzaldehyde . Although the sensitivity to benzaldehyde was often associated with that for the leaf alcohols , examples of both benzaldehyde insensitive cells -LRB- cell 14 -- 15 -RRB- and apparent benzaldehyde specialists -LRB- cells 18 -- 19 -RRB- were found . Fourteen cells responded selectively to CO2 -LRB- Fig. 8 -RRB- . The dose -- response curve -LRB- Fig. 9 -RRB- is almost linear from 0.2 to 2.5 % CO2 . No significant response to other stimuli was found for these cells . All tested C6 compounds sometimes inhibited spike activity in CO2 cells , but only at high concentrations -LRB- 10 − 1 v/v -RRB- . The terpenoids limonene , citral , and geraniol never inhibited the CO2 cells -LRB- n = 14 -RRB- . Fig. 8Response of a CO2 sensitive cell in the maxillary palp of Yponomeuta cagnagellus . a to e are responses to , respectively , 0.26 , 1.0 , 1.5 , 2.5 , and 10 % CO2 . The time marker indicates 1 sFig . 9Dose -- response relationship of 12 CO2 sensitive cells in the palp of Yponomeuta cagnagellus stimulated with CO2 or clean air . Vertical bars indicate 95 % confidence interval Three cells did not react to chemical stimuli but responded to changes in temperature induced by a hot or cold metal rod in the vicinity of the palp . A drop in temperature evoked a strong response . An increase in temperature inhibited the cells -LRB- Fig. 10 -RRB- , i.e. these cells functioned as cold receptors . Fig. 10Response of a cold receptor in the maxillary palp of Yponomeuta cagnagellus . a Stimulation with a cold metal rod at an upwind distance of about 10 mm . b Stimulation with a warm metal rod at an upwind distance of about 10 mm . Stimulus bar 2.5 s Feeding behaviour modulated by benzaldehyde Feeding assays were conducted for Y. cagnagellus at 5 different concentrations of benzaldehyde on 72 insects per treatment -LRB- Fig. 11 -RRB- . Benzaldehyde had a positive feeding index at concentrations of 0.5 × 10 − 3 , 0.2 × 10 − 2 , and 0.5 × 10 − 2 . However , the stimulating effect of benzaldehyde was significant only at the concentration of 0.2 × 10 − 2 M , with a feeding index of about 12 % -LRB- percentage leaf disk left on benzaldehyde and control was , respectively , 60.9 ± 5.3 and 76.8 ± 4.4 % , Mann -- Whitney U = 427 , P = 0.013 -RRB- . At higher concentrations , benzaldehyde tended to inhibit the feeding of Y. cagnagellus larvae . For example , the feeding index was − 3 % at the concentration of 0.5 × 10 − 1 but feeding inhibition was not significant compared to the control group . Fig. 11Feeding index of three Yponomeuta species . FI = -LRB- C − T -RRB- / -LRB- C + T -RRB- -LRB- ranging from − 100 to 100 % -RRB- . C and T denote , respectively , the mean of the remaining leaf surface in the control group and in the treatment -LRB- benzaldehyde -RRB- group . Feeding indexes with P < 0.05 are indicated with an asterisk The feeding response of Y. padellus was tested at four concentrations , i.e. 0.5 × 10 − 4 , 0.5 × 10 − 2 , 1 × 10 − 2 , and 0.5 × 10 − 1 -LRB- Fig. 11 -RRB- . Benzaldehyde significantly stimulated the feeding of caterpillars at all concentrations except at the lowest concentration -LRB- percentage leaf disk left on benzaldehyde and control at 0.5 × 10 − 2 was , respectively , 76.5 ± 3.7 and 84.5 ± 3.4 % , Mann -- Whitney U 375 , P = 0.003 , At 1 × 10 − 2 76.6 ± 3.8 and 90.4 ± 1.7 % , Mann -- Whitney U = 321 , P = 0.001 and at 0.5 × 10 − 1 70 ± 3.8 % and 82.8 ± 3.3 % Mann -- Whitney U = 408 , P < 0.007 -RRB- . In Y. plumbellus , no effect of benzaldehyde on food intake was found , and the insects appeared to be fully indifferent to the compound -LRB- Fig. 11 -RRB- . Discussion Morphology A description of the external structure of the maxillary palps of ditrysian larvae is given by Grimes and Neunzig -LRB- 1986 -RRB- . From their results it is clear that location , size , and shape of palpal sensilla are constant in lepidopterous larvae . Eight sensilla are always found on the apex . In addition , a digitiform sensillum and up to four campaniform sensilla or pore plates are present in the wall of the most distal segment . Grimes and Neunzig -LRB- 1986 -RRB- make a distinction between the more primitive , endophagous feeders , and larger , exophagous feeders . In endophagous species , the walls of the A sensilla are smooth , and A2 is often two-tiered -LRB- styloconic -RRB- . In the more advanced , exophagous groups A2 is basiconic and the A sensilla are wrinkled or pockmarked . The Yponomeutidae are regarded as relatively primitive Ditrysia -LRB- Kristensen and Skalski 1999 -RRB- , and indeed a styloconic A2 and smooth A sensilla are found -LRB- Fig. 4 ; see also van Drongelen 1979 -RRB- . On morphological grounds a gustatory function for L and M sensilla has been suggested -LRB- Grimes and Neunzig 1986 -RRB- , and for Choristoneura fumiferana this was confirmed by recordings -LRB- Albert 2003 -RRB- . In Helicoverpa armigera , however , some sensilla show ultrastructural features characteristic for both olfaction and taste -LRB- Keil 1996 -RRB- and it is therefore difficult to assign a function based on mophology alone . Although we recorded responses to gustatory as well as olfacory stimuli , we stimulated all eight sensilla simultaneously and this method precludes assignment of the observed responses to particular sensilla . The wall of the most distal segment of the palp bears at least one campaniform sensillum . This sensillum is probably equivalent to the large plate sensillum described in C. fumiferana -LRB- Albert 2003 -RRB- , Euxoa messoria -LRB- Devitt and Smith 1982 -RRB- , and H. amigera -LRB- Keil 1996 -RRB- . The plate sensilla are multiporous , multiple innervated , and possess branching dendrites . If this is also true of Y. cagnagellus , these sensilla are presumably olfactory organs where some of the neural responses reported in this paper could originate . In the wall of the most distal segment , a digitiform sensillum is present which is normally innervated by one neuron -LRB- Schoonhoven and Dethier 1966 ; Albert 1980 ; Devitt and Smith 1982 ; Keil 1996 -RRB- . In E. messoria and Heliotis amigera this sensillum appears to possess a laminated outer dendritic segment . Such laminated dendrites have been associated with cold receptors -LRB- see Altner and Loftus 1985 -RRB- , as well as with CO2 receptors -LRB- Chu-wang et al. 1975 ; McIver and Siemicki 1984 ; Lee et al. 1985 ; Bogner et al. 1986 -RRB- . In Y. cagnagellus both cold receptors and CO2 receptors were found . The digitiform sensillum of Y. cagnagellus might therefore house a CO2 receptor as suggested by Keil -LRB- 1996 -RRB- but without further electrophysiological evidence cold reception can not be excluded . Carbon dioxide reception The ecological function of the CO2 receptor in Y. cagnagellus is not clear . CO2 reception is well known in soil dwelling organisms that must locate plant roots -LRB- Johnson and Gregory 2006 -RRB- . CO2 sensitivity is also common in adult lepidoptera -LRB- Bogner 1990 ; Stange and Stowe 1999 -RRB- . Helicoverpa armigera for instance is in principle capable of detecting modulation of CO2 concentrations normally occurring in the environment -LRB- Stange 1992 -RRB- . The CO2 receptor of Y. cagnagellus and other lepidopterous larvae is clearly less sensitive than that of adult Lepidoptera , however , CO2 receptors with a similar low sensitivity range have been described in the tick Amblyomma varigatum -LRB- Steullet and Guerin 1992 -RRB- . During the day , CO2 levels near the surface of green leaves will be 0.038 % or lower , while this level may rise to a maximum of 1.2 % during nocturnal respiration -LRB- see Bogner 1990 for references -RRB- . Although the latter concentration is just within the range of the larval receptor , it seems unlikely that the CO2 receptor is used to assess subtle changes in the respiratory status of the leaf -LRB- as a messure of leaf quality -RRB- . Alternative hypotheses are that the receptor is used to monitor CO2 levels in the communal nest during periods of tight clustering , or that it facilitates aggregation behaviour . However , as yet experimental data to support these ideas is lacking . Temperature perception Only qualitative data was collected from the three observed cold receptors . The actual drop in temperature induced by the hot and cold metal rods used , as stimulus is not known . The cold receptors might be used for monitoring the microclimate -LRB- Schoonhoven 1967 -RRB- and in principle could be used to determine smaller temperature variations associated with leaf quality -LRB- Dethier and Schoonhoven 1968 -RRB- . Plant odour responses Stimulation with plant odours yielded cells of at least two spectral types : aldehyde-sensitive cells and alcohol-sensitive cells . The two groups are clearly separated from each other . However , within each group responses to several chemically very different stimuli were found . A single receptor cell that reacts to different types of compounds used to be interpreted as a reflection of the presence of several receptor `` sites '' on the membrane -LRB- Kaissling 1976 ; Selzer 1984 ; Hansen 1978 ; Wiezoreck and Wolff 1989 -RRB- . With the identification of the actual receptor genes and advanced labeling techniques it was suggested that normally only a single receptor protein is expressed in a particular sensory cell , the `` one receptor -- one neuron '' rule -LRB- see Gaillard et al. 2004 for review -RRB- . Recently however , it was shown that in Drosophila olfactory organs this rule does not always hold , and coexpression of different receptor proteins was observed -LRB- Goldman et al. 2005 -RRB- . In C. elegans coexpression must also occur , simply because there are too many receptor genes to express for the limited number of sensory neurons . We observed single cells that responded to structurally different compounds such as -LRB- Z -RRB- -3-hexen-1-ol and benzaldehyde , but also cells that responded to only one of these two compounds . These observations can in each case still be explained by the expression of a single receptor protein per cell , with a different binding specificity -LRB- i.e. a cell that responds to several compounds expresses a receptor with a broad specificity , while a cell that responds to only a single compound expresses a narrowly tuned receptor protein -RRB- . Alternatively , cells might be broadly tuned because they express an assembly of several narrowly tuned receptor proteins with different sensitivity spectra . In either case , following this line of reasoning a simple quantitative shift in the numbers of receptor proteins could in principle result in a qualitative change in host-acceptance behaviour and host shifts , processes that were postulated as major factors in the speciation process in Yponomeuta species -LRB- Menken et al. 1992 ; Menken and Roessingh 1998 -RRB- . From Fig. 6 is clear that although at least two groups of cells with comparable spectral responses can be distinguished -LRB- cells 1 -- 5 , cells 6 -- 19 -RRB- , however , there are no sharp boundaries between these classes -LRB- cf. cell 5 -RRB- , and considerable variation does exist . Since care was taken to record only from cells that were reliable and reproducibly responding , it is likely that this variation does reflect natural variability in the receptor cell population . The separation in aldehyde - and alcohol sensitive cells should therefore at present merely be considered as a convenient way to summarise the data . Until more recordings are made , it can not be excluded that a continuum from more specialised to , more generalised cells exists . In the present study , no cells with a primary response to -LRB- Z -RRB- -3-hexenyl acetate were found in the maxillary palps of Y. cagnagellus , but receptors sensitive to this compound have been found on the antennae of this species -LRB- Roessingh , unpublished results -RRB- as well as on the antennae of Pieris brassicae larvae -LRB- Visser and de Jong 1988 -RRB- . In this respect it is interesting to note that in Manduca sexta fibers from the maxillary nerve travel into a core of neuropile in the suboesophageal ganglion that also receives antennal axons -LRB- Kent and Hildebrand 1987 -RRB- . The maxillary palps of caterpillars contain 15 -- 30 chemoreceptors -LRB- Schoonhoven and Dethier 1966 ; Albert 1980 ; Devitt and Smith 1982 -RRB- . If the 85 cells in the present study represent a random sample from the population in the palps , then the proportion of neurons sensitive to each of the tested stimuli could be estimated . However , it must be stressed that the position of the electrode was not reproducable between recordings . As a consequence , the recordings by no means represent a random sample . In spite of this limitation it can be concluded that receptors for the tested terpenoids and for -LRB- Z -RRB- -3-hexen-1-yl acetate are relatively scarce , since no cells primarily sensitive to these stimuli were encountered . The role of benzaldehyde in feeding behaviour Because benzaldehyde is absent from E. europaeus , Euonymus feeders such as Y. plumbellus and Y. cagnagellus are not expected to be sensitive to this compound , or use it as a repellent . The electrophysiological results clearly show that benzaldehyde is stimulating the sensory cells in the maxillary palp of Y. cagnagellus ; the straightforward interpretation would be that in Y. cagnagellus benzaldehyde signals a non-host compound , and therefore low acceptability . On the other hand , as benzaldehyde is prominently present in Rosaceae , it can be expected to be a feeding stimulant for Rosaceae feeders like Y. padellus , a species that is closely related to Y. cagnagellus . From a phylogenetic perspective a different hypothesis can thus be formulated . Yponomeuta cagnagellus is located within the European clade that feeds mostly on Rosaceae , which do contain benzaldehyde . Considering that the most recent common ancestor of this clade was presumably a Rosaceae feeder , the sensitivity of Y. cagnagellus to benzaldehyde could be viewed as a maintained sensitivity to a feeding stimulant from the ancestral host . Under this hypothesis both Y. cagnagellus and Y. padellus are expected to be stimulated by benzaldehyde . Yponomeuta plumbellus , a Celastraceae feeder outside the European clade , is expected to be unresponsive because it has never been associated with Rosaceae nor have its ancestors . The results from the feeding experiment obtained from the three species are consistent with the latter scenario . Benzaldehyde stimulated feeding in Y. cagnagellus and Y. padellus and increased food intake by 6 -- 12 % . Yponomeuta plumbellus did not respond to benzaldehyde at all . The sensory response to benzaldehyde in Y. cagnagellus is therefore probably an evolutionary relict of the association with Rosaceae of the ancestral lineage of this species . In a previous study -LRB- van Drongelen 1979 -RRB- , dulcitol sensitivity was found in Y. evonymellus and Y. padellus , species feeding on Rosaceae that do contain very little or nodulcitol . In this case it was similarly concluded that the dulcitol sensitivity might be an evolutionary relict from the ancestral host association with Celastraceae . Although feeding in both Y. cagnagellus and Y. padellus was stimulated by benzaldehyde , Y. padellus -LRB- associated with a benzaldehyde-containing hosts -RRB- responded over a much larger concentration range than Y. cagnagellus -LRB- a species that is using a benzaldehyde-free host -RRB- . It should be noted that care must be taken when interpreting these results . It is not clear what the natural concentrations of host plant odours that are experienced by the larvae , since a steep gradient in the boundary layer exists , and concentrations near the leaf surface can be much higher than those obtained from headspace samples -LRB- Schoonhoven et al. 2005 -RRB- . However , since the sensory responses show good dose -- response relations , and the behaviour yields a clear optimum , we feel that the used concentrations are covering the natural range . It will be interesting to investigate the actual genetics underlying the observed sensitivity differences between the species .

O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B I O B O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B I O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B I O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B I O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O 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O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B I O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B I O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B I O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O 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O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B I O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B I O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B I O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B I O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B I O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B I O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B I O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B I O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B I O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B I O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O

sensory physiology
plant odours
yponomeuta cagnagellus
adaptive speciation
lepidoptera


Structure of the Tandem Fibronectin Type 3 Domains of Neural Cell Adhesion Molecule Activation of the fibroblast growth factor receptor -LRB- FGFR -RRB- by neural cell adhesion molecule -LRB- NCAM -RRB- is essential for NCAM-mediated neurite outgrowth . Previous peptide studies have identified two regions in the fibronectin type 3 -LRB- FN3 -RRB- - like domains of NCAM as being important for these activities . Here we report the crystal structure of the NCAM FN3 domain tandem , which reveals an acutely bent domain arrangement . Mutation of a non-conserved surface residue -LRB- M610R -RRB- led to a second crystal form showing a substantially different conformation . Thus , the FN3 domain linker is highly flexible , suggesting that it corresponds to the hinge seen in electron micrographs of NCAM . The two putative FGFR1-binding segments , one in each NCAM FN3 domain , are situated close to the domain interface . They form a contiguous patch in the more severely bent conformation but become separated upon straightening of the FN3 tandem , suggesting that conformational changes within NCAM may modulate FGFR1 activation . Surface plasmon resonance experiments demonstrated only a very weak interaction between the NCAM FN3 tandem and soluble FGFR1 proteins expressed in mammalian cells -LRB- dissociation constant > 100 μM -RRB- . Thus , the NCAM -- FGFR1 interaction at the cell surface is likely to depend upon avidity effects due to receptor clustering . Introduction The neural cell adhesion molecule -LRB- NCAM -RRB- is the prototype and founding member of the immunoglobulin -LRB- Ig -RRB- superfamily cell adhesion molecules -LRB- CAMs -RRB- .1 -- 4 NCAM is present on the cell surface of neurons , astrocytes and oligodendrocytes , where it mediates homophilic and heterophilic cell adhesion . NCAM is involved in neuronal migration , axon growth and guidance , as well as in synaptic plasticity associated with learning and memory .5 -- 7 Alternative splicing of the NCAM1 gene results in isoforms of three size classes that differ in their membrane attachment and cytosolic regions but have in common an extracellular domain consisting of five Igs and two fibronectin type 3 -LRB- FN3 -RRB- domains .8 The two larger isoforms have a transmembrane helix and cytosolic domains of different sizes , while the smallest isoform has a glycophosphatidylinositol membrane anchor . Variable use of alternative exons in the extracellular domain results in small insertions into Ig4 or between the FN3 domains .9 -- 12 NCAM function is further regulated by an unusual posttranslational modification , namely , the addition of polysialic acid to Ig5 .13 The molecular basis of homophilic adhesion by NCAM has been a subject of intense study , and the results from biochemical and biophysical studies have not always been consistent .14,15 A crystal structure of NCAM Ig1 -- Ig3 has led to a zipper model of adhesion , which postulates both cis and trans interactions by the Ig1 -- Ig3 region of NCAM .16 While a conclusive picture has yet to emerge , it appears that there may be multiple modes of homophilic interaction .17 NCAM is also engaged in heterophilic interactions . There is now convincing evidence that NCAM-mediated neurite outgrowth , as well as tumour development and progression , critically involves the activation of fibroblast growth factor receptor 1 -LRB- FGFR1 -RRB- , through a cis interaction of NCAM and FGFR1 .18 -- 22 The four FGFRs and their 23 growth factor ligands control a variety of cellular processes , including development , angiogenesis , hematopoiesis and tumourigenesis .23 -- 26 Alternative splicing of the four FGFR genes results in at least 48 receptor isoforms that vary in their ligand binding profiles and kinase domains . The longest FGFR1 ectodomain variant consists of three Ig domains , D1 -- D3 , with a stretch of acidic amino acids -LRB- the `` acid box '' -RRB- inserted into the D1 -- D2 linker ; shorter forms lack D1 and the acid box . The NCAM -- FGFR1 interaction was originally proposed based on indirect biological evidence ,27 but biochemical evidence has been obtained to suggest that the interaction is mediated by direct binding of the FN3 domains of NCAM to FGFRs -LRB- Fig. 1 -RRB- .20,28,29 We and others identified NCAM-derived peptides capable of stimulating FGFR1 signalling and inducing neurite outgrowth . One peptide -LRB- FRM peptide -RRB- is derived from the first FN3 domain of NCAM -LRB- 1FN3 -RRB- ,30 while another -LRB- FGL peptide -RRB- is derived from the second FN3 domain -LRB- 2FN3 -RRB- .28 The structures of the 1FN3 and 2FN3 domains in isolation have been determined ,28,31 but how the two domains cooperate in FGFR1 activation is unclear . In this study , we determined the crystal structure of the NCAM FN3 tandem -LRB- 1FN3 -- 2FN3 -RRB- in two crystal forms . We report that 1FN3 -- 2FN3 assumes a bent conformation in both forms , with evidence of substantial flexibility of the domain linker . In direct binding experiments with fully glycosylated proteins of mammalian origin , we observed only a very weak interaction -LRB- dissociation constant > 100 μM -RRB- of NCAM 1FN3 -- 2FN3 with two FGFR1 ectodomain constructs . We conclude that the NCAM -- FGFR1 interaction at the cell surface may be transient or stabilised by avidity effects resulting from receptor clustering and that conformational changes within NCAM may have a profound role in FGFR1 activation . Results Crystal structure of the NCAM FN3 tandem To obtain insight into the relative orientation of the two FN3 domains of NCAM , we determined the crystal structure of 1FN3 -- 2FN3 at 2.3-Å resolution -LRB- Table 1 -RRB- . Both 1FN3 and 2FN3 adopt the typical β-sandwich fold of all FN3 domains consisting of seven strands arranged in two antiparallel sheets -LRB- ABE and GFCD -RRB- -LRB- Fig. 2a and b -RRB- . Preceding strand A in both domains are short proline-rich segments that are integrated into the FN3 fold , with the proline tetrahydropyrrole rings pointing into the hydrophobic core -LRB- Pro500 and Pro503 in 1FN3 ; Pro601 and Pro604 in 2FN3 -RRB- . A similar feature has been observed in other FN3 domains -LRB- e.g. , in gp13033 and titin34 -RRB- . 1FN3 contains an unusual α-helix situated between strands D and E , as reported previously .31 1FN3 in our FN3 tandem structure matches the crystal structure of 1FN3 in isolation ,31 with an r.m.s.d. of 0.50 Å for 100 Cα atoms . 2FN3 in our FN3 tandem matches the solution structure of 2FN3 in isolation ,28 with an r.m.s.d. of 1.3 Å for 92 Cα atoms -LRB- the main differences are concentrated in the B -- C and C -- D loops -RRB- . The relative orientation of the two FN3 domains in the 1FN3 -- 2FN3 tandem is characterised by an unusually bent conformation with an interdomain angle of ∼ 80 ° -LRB- calculated between the long axes of the two FN3 domains -RRB- . The interface between 1FN3 and 2FN3 buries 630 Å2 of solvent-accessible surface -LRB- calculated with the CCP4 program AREAIMOL -RRB- , which is in the typical range for rigid FN3 -LRB- and Ig -RRB- domain interfaces . The interface is dominated by polar interactions , between the A -- B loop of 1FN3 on the one hand and the domain linker and B -- C loop of 2FN3 on the other -LRB- Fig. 2c -RRB- The key interface residues -LRB- Tyr511 , Ser513 , Thr514 , Pro601 and Asp625 -RRB- are strictly conserved in all vertebrate NCAM sequences -LRB- Fig. 2d -RRB- , suggesting that the bent conformation may be physiologically relevant . However , residues 511 -- 514 have also been implicated in FGFR1 binding ,30 and the linker region could assume a very different structure when NCAM is bound to FGFR1 -LRB- see below -RRB- . The conformation of multidomain proteins often is influenced by the crystal lattice . In this regard , we noted that the asymmetric unit of our crystals contains two 1FN3 -- 2FN3 molecules arranged in a tightly interlocked dimer -LRB- Fig. 2b -RRB- . There are two main contacts responsible for dimer formation : the α-helix of 1FN3 packs against the GFCD sheet of 2FN3 of the other molecule , and the 2FN3 domains of the two molecules interact via their A and G strands . Altogether , these contacts bury as much as 3040Å2 of solvent-accessible surface . We do not think that the dimer observed in our crystals is physiologically relevant , as dimer interface residues are only poorly conserved -LRB- Fig. 2d -RRB- and NCAM dimers were never observed in electron microscopy studies .35,36 Because we were concerned that the tight association of 1FN3 -- 2FN3 molecules in the dimer may have forced the unusually bent interdomain conformation , we sought to disrupt the dimer by mutagenesis and crystallise a mutant 1FN3 -- 2FN3 protein in a different crystal form . Structure of the M610R mutant We expressed three point mutants of NCAM 1FN3 -- 2FN3 -LRB- M610R , Y672E and R690E -RRB- , all of which should be incompatible with the dimer structure seen in crystals of the wild-type protein . Importantly , all three mutations target surface residues and are unlikely to have an effect on NCAM folding . Whether the mutations are functionally neutral could not be determined due to a lack of suitable assays . When examined by size-exclusion chromatography , all three mutants eluted as a mixture of monomers and dimers , similar to the wild-type protein -LRB- data not shown -RRB- . We obtained crystals of the M610R mutant and determined its structure at 2.7-Å resolution by molecular replacement -LRB- Fig. 3a -RRB- . The asymmetric unit of the crystals contains a hexamer of NCAM 1FN3 -- 2FN3 M610R . The hexamer can be regarded as a trimer of dimers , with the dimers having a completely different mode of association compared with the wild-type structure -LRB- the individual domains are very similar , as expected , with r.m.s.d. values of 0.93 and 1.1 Å for 1FN3 and 2FN3 , respectively -RRB- . The relative orientation of the two FN3 domains in the M610R mutant is less severely bent than in the wild-type structure but still far from fully extended -LRB- interdomain angle ∼ 120 ° ; Fig. 3b -RRB- . The FN3 pair opens up in the M610R mutant , and 1FN3 is additionally twisted about its long axis . The combined hinge opening and 1FN3 twisting amounts to a pure rotation of 73 ° for 1FN3 when the structures are superimposed on their 2FN3 domains . There is no domain interface to speak of in the mutant -LRB- 240 Å2 buried -RRB- , and the conformation appears to be stabilised entirely by the crystal lattice . Thus , crystal lattice forces can have a profound influence on the conformation of the NCAM FN3 tandem , suggesting that the 1FN3 -- 2FN3 linker may act as a flexible hinge in native NCAM . We compared the NCAM FN3 tandem with other FN3 tandems of known structure . Tandems from the extracellular matrix proteins fibronectin and tenascin generally assume an extended conformation .38 -- 40 In contrast , many cytokine and hormone receptors -LRB- e.g. , gp130 -RRB- 33 feature bent FN3 pairs that are superficially similar to the NCAM structures reported here -LRB- data not shown -RRB- . Of particular interest is the structure of the FN3 pair of neuroglian , a Drosophila CAM .37 The neuroglian tandem , which has an extensive domain interface that incorporates a bound sodium ion , adopts a conformation that is intermediate between the two conformations we report for the NCAM tandem . Thus , a similar bend in the FN3 linker -LRB- s -RRB- may be a general feature of other animal CAMs containing FN3 domains in their membrane-proximal region . Location of putative FGFR1 binding site Previous studies have implicated two NCAM 1FN3 -- 2FN3 regions in FGFR1 binding . Kiselyov et al. identified a bioactive peptide from the F -- G loop of 2FN3 -LRB- FGL peptide -RRB- ,28 and we identified a bioactive peptide from the A -- B loop of 1FN3 -LRB- FRM peptide -RRB- .30 In agreement with our earlier prediction ,30 the FRM and FGL loops are indeed located in close proximity on the same face of the wild-type NCAM molecule -LRB- Fig. 4a -RRB- . However , in the M610R mutant , the opening up of the two domains combined with rotation of 1FN3 places the FRM and FGL loops much farther apart . Thus , conformational changes at the NCAM 1FN3 -- 2FN3 hinge may modulate the interaction of NCAM with FGFR1 . Surface plasmon resonance analysis of the NCAM -- FGFR1 interaction We wanted to map the FGFR1 binding site on the NCAM FN3 tandem by structure-based mutagenesis and first sought to establish a suitable binding assay . A solid-phase assay with immobilised NCAM and Fc-tagged FGFR1 proteins did not show any appreciable interaction -LRB- data not shown -RRB- . We therefore used surface plasmon resonance -LRB- SPR -RRB- to analyse the binding of NCAM 1FN3 -- 2FN3 to two FGFR1 ectodomain constructs . The FGFR1 D1 -- D3 construct used -LRB- residues 22 -- 364 -RRB- spans essentially the full ectodomain and contains the acid box situated between domains D1 and D2 . The FGFR1 D2 -- D3 construct used -LRB- residues 151 -- 364 -RRB- lacks D1 and the acid box but retains the binding site for FGFs ; this construct is similar to the construct previously used by Kiselyov et al. in SPR studies .28 Both soluble FGFR1 proteins were produced by the 293-EBNA cells in good yields . Due to the presence of multiple N-linked glycosylation sites in FGFR1 -LRB- see below -RRB- , the purified recombinant proteins migrate as diffuse bands of higher-than-calculated molecular mass on SDS-PAGE -LRB- Fig. 5 -RRB- . In a first set of experiments , the two FGFR1 constructs were immobilised on a CM5 sensor chip -LSB- 8000 resonance units -LRB- RU -RRB- of D1 -- D3 and 3850 RU of D2 -- D3 -RSB- . Recombinant FGF1 injected at a concentration of 100 nM produced sensorgrams characteristic of a high-affinity interaction , confirming that the immobilised proteins are functional -LRB- Fig. 6a and b -RRB- . In contrast , wild-type NCAM 1FN3 -- 2FN3 up to a concentration of 70 μM did not produce a signal on the FGFR1 D1 -- D3 surface and showed only very weak binding to FGFR D2 -- D3 -LRB- Fig. 6c and d -RRB- . In a second set of experiments , the order of proteins was reversed . NCAM 1FN3 -- 2FN3 proteins were immobilised on a CM4 sensor chip -LRB- 1800 RU of wild-type protein and 1900 RU of M610R mutant -RRB- , and the two soluble FGFR1 constructs were used as analytes up to a concentration of 100 μM . We again observed only weak interactions for all pairings -LRB- Fig. 6e -- h -RRB- . Wild-type and M610R NCAM 1FN3 -- 2FN3 behaved almost identically in these experiments , and , as before , it appeared that the affinity of NCAM for FGFR1 D2 -- D3 was higher than that for FGFR1 D1 -- D3 . The fast association and dissociation steps in the sensorgrams prevented the fitting of kinetic constants . We used the plateau values at equilibrium to estimate a dissociation constant of > 100 μM for the interaction of NCAM 1FN3 -- 2FN3 with FGFR1 D2 -- D3 -LRB- not shown -RRB- , but we emphasise that this value is very approximate given the weak resonance signals obtained . In view of the weakness of the NCAM -- FGFR1 interaction in our assay , we were unable to pursue our initial plans of mapping the binding site -LRB- s -RRB- by mutagenesis . Discussion NCAM ectodomain structure The current view of the NCAM ectodomain structure is based on early studies by rotary shadowing electron microscopy of tissue-derived NCAM .35 Electron microscopy visualised the NCAM ectodomain as ∼ 28-nm rods bent at a flexible hinge located ∼ 10 nm from the C-terminus ; the angle between the two arms varied from 50 ° to 140 ° -LRB- average = 100 ° -RRB- . The hinge was attributed to the proline-rich linker between Ig5 and the first FN3 domain , and the long and short arms were described as rigid domain tandems -LRB- Ig1 -- Ig5 and 1FN3 -- 2FN3 , respectively -RRB- in extended conformations .35 A crystal structure of NCAM Ig1 -- Ig3 indeed showed a largely extended structure .16 In sharp contrast , the present crystal structure analysis of the NCAM 1FN3 -- 2FN3 domain pair has revealed a prominent bend between the two domains in two independent crystal forms -LRB- Fig. 3b -RRB- . This finding is difficult to reconcile with the uniformly straight appearance of the short arm in the electron micrographs and suggests that the hinge point may actually lie between 1FN3 and 2FN3 . In this respect , we note that the Ig5 -- 1FN3 linker is actually rather short and may well be rigid -LRB- in the Ig5 -- 1FN3 linker sequence , ILVQADTPSSP , the isoleucine and valine residues are predicted to contribute to the hydrophobic core of Ig5 and the first proline is already part of the 1FN3 fold -RRB- . If the hinge is instead situated in the 1FN3 -- 2FN3 linker , the juxtamembrane domain of NCAM must have contributed to the short arm seen in electron micrographs , as a single FN3 domain would only account for half of the short arm length . The serine/threonine-rich juxtamembrane domain of NCAM -LRB- sequence TSAQPTAIPANGSPTSGLSTGA -RRB- is predicted to be extensively modified by O-linked glycosylation -LRB- NetOGlyc 3.1 server † -RRB- and could easily assume the extended and rigid conformation required to span the remaining ∼ 5 nm .41 Further structural analysis , in particular , of the Ig5 -- 1FN3 pair , is required to conclusively pinpoint the site of articulation within the NCAM ectodomain . NCAM splice variants Interestingly , the flexible hinge linking the two FN3 domains of NCAM 1FN3 -- 2FN3 is known to be modified by alternative splicing . Our structure is of the shortest isoform -LRB- linker sequence TQPVREPSAP -RRB- , whereas the underlined arginine residue is replaced by QG , HSPPPQG or even longer sequences in other variants .9,10,12 The HSPPPQG insertion has been suggested as a potential hinge region .1 The biological relevance of these splicing events is not well understood , but in vitro experiments have demonstrated that the NCAM isoforms differ in their capacity to support cell adhesion and spreading ,42,43 as well as myoblast fusion .44 We prepared NCAM 1FN3 -- 2FN3 proteins with QG or HSPPPQG inserts in the domain linker , but , unfortunately , these proteins were very prone to aggregation in physiological buffers and could not be used for SPR or structural analysis -LRB- data not shown -RRB- . Interaction of NCAM with FGF Hinge bending and alternative splicing at the 1FN3 -- 2FN3 junction could affect NCAM function by modulating either its homophilic binding properties or its heterophilic interactions with other proteins . There is currently no evidence for the former scenario . In contrast , the functional interaction of NCAM with FGFR1 in cis -LRB- i.e. , at the same cell membrane -RRB- is well established1 ,22,30 and would appear to be an attractive candidate for regulation by alternative splicing . Peptides from two regions of the NCAM 1FN3 -- 2FN3 tandem , one in each domain , have been shown to modulate FGFR1-dependent neurite outgrowth .28,30 Intriguingly , the corresponding loop regions are in close proximity in the acutely bent conformation observed in crystals of the wild-type protein , suggesting that they are part of a larger FGFR1 binding site extending over the domain junction . In crystals of the M610R mutant , the two putative FGFR1-binding loops are farther apart and no longer on the same face of the 1FN3 -- 2FN3 structure -LRB- Fig. 4 -RRB- . Thus , changes in the 1FN3 -- 2FN3 conformation , either by biomechanical forces resulting from cell -- cell contact or by alternative splicing , could have a profound effect on the NCAM -- FGFR1 interaction . Other NCAM activities that might be affected by alternative splicing of the 1FN3 -- 2FN3 linker include interactions with polysialyltransferases31 and prion protein ,45 which both bind to the FN3 domains . Previous biochemical studies reported a dissociation constant of ∼ 10 μM for the interaction between NCAM 1FN3 -- 2FN3 and FGFR1 D2 -- D3 .28,29 Another study , using cell-based assays , concluded that the acid box in the FGFR1 D1 -- D2 linker was essential for the NCAM -- FGFR1 interaction .20 We wanted to use SPR binding experiments to identify NCAM residues involved in FGFR1 binding and test the effect of splice inserts in the 1FN3 -- 2FN3 linker . Unfortunately , using our recombinant proteins expressed in mammalian cells -LRB- Fig. 5 -RRB- , we were unable to detect substantial binding between soluble FGFR1 and NCAM proteins , regardless of whether FGFR1 or NCAM was immobilised on the sensor chip . A very weak interaction -LRB- estimated dissociation constant > 100 μM -RRB- was evident between FGFR1 D2 -- D3 and NCAM 1FN3 -- 2FN3 , but we observed no NCAM binding to the FGFR1 D1 -- D3 construct containing the acid box -LRB- Fig. 6 -RRB- . The most likely explanation for the discrepancy between our findings and those of Christensen et al. 29 is the difference in glycosylation of the FGFR1 proteins used . Christensen et al. expressed FGFR1 D2 -- D3 in insect cells , which produce N-linked oligosaccharides of the high-mannose type , whereas our expression system -LRB- human embryonic kidney cells -RRB- produces complex-type oligosaccharides , which more closely resemble the glycan present on mammalian FGFRs . Human and rodent FGFR1 proteins are highly glycosylated , and the glycan is known to influence ligand binding .46 We think that there may be electrostatic repulsion between the acidic NCAM 1FN3 -- 2FN3 protein -LRB- isoelectric point 5.0 -RRB- and the terminal sialic acids present on FGFR1 expressed in human cells . The very weak interaction we observed between soluble NCAM and FGFR1 proteins does not preclude a critical role of this interaction at the cell surface , where avidity effects due to receptor clustering may be substantial . An attractive hypothesis is that the functional state of NCAM -LRB- i.e. , whether it is engaged in a homophilic contact or not -RRB- is linked to FGFR1 binding and activation . It is tempting to speculate that the flexible 1FN3 -- 2FN3 linkage revealed by our structural analysis could provide the molecular means for such a regulatory mechanism . Experimental Procedures Expression vectors NCAM constructs were made by PCR amplification from a bacterial expression vector coding for the FN3 pair of human NCAM . Our NCAM numbering scheme corresponds to SwissProt entry P13591 up to residue 598 but differs by − 1 from P13591 for all subsequent residues due to the replacement of Gln599 -- Gly600 by Arg , a naturally occurring splice variant in the brain and muscle .12 The M610R mutation in NCAM was introduced by strand-overlap-extension PCR . FGFR1 constructs were made by PCR amplification from a complete cDNA clone of human FGFR1 -LRB- IIIc isoform ; our numbering scheme corresponds to SwissProt entry P11362 -RRB- . The PCR products were cloned into a modified pCEP-Pu vector47 coding for proteins with a C-terminal His6 tag . The insert sequences of all expression vectors were verified by DNA sequencing . The domain boundaries of the constructs are as follows : NCAM 1FN3 -- 2FN3 , QADTP ... VFRTS -LRB- 496 -- 692 -RRB- ; FGFR1 D1 -- D3 , RPSPT ... EALEE -LRB- 22 -- 364 -RRB- ; and FGFR1 D2 -- D3 , VAPYW ... EALEE -LRB- 151 -- 364 -RRB- . Vector-derived APLA and AAAHHHHHH sequences are additionally present at the N - and C-terminus , respectively . Protein expression and purification All proteins were purified from the conditioned medium of episomally transfected 293-EBNA cells . Cells were cultured in Dulbecco 's modified Eagle 's medium supplemented with 10 % fetal calf serum -LRB- Invitrogen -RRB- , transfected using Fugene reagent -LRB- Roche Applied Science -RRB- and selected with 1 μg/ml of puromycin -LRB- Sigma -RRB- . Proteins were purified by a combination of affinity and size-exclusion chromatography performed on an Äkta platform -LRB- GE Healthcare -RRB- . Typically , 1.5 l of conditioned serum-free medium was loaded onto a 5-ml HisTrap column -LRB- GE Healthcare -RRB- equilibrated in phosphate-buffered saline -LRB- PBS -RRB- buffer , pH 7.45 -LRB- 140 mM NaCl , 10 mM Na2PO4 and 3 mM KCl -RRB- , and eluted with 500 mM imidazole in PBS . The eluate was concentrated using Vivaspin centrifugal devices -LRB- Sartorius AG -RRB- and further purified on a 24-ml Superdex 200 size-exclusion chromatography column -LRB- GE Healthcare -RRB- with Tris-buffered saline -LRB- TBS -RRB- buffer , pH 7.4 , as the running buffer . Purified proteins were analysed by SDS-PAGE , quantified by measuring their absorption at 280 nm , concentrated to the final desired concentrations and flash-frozen in liquid nitrogen for storage at − 80 °C . Final yields were 10 -- 20 mg of pure protein per litre of cell culture medium . Crystallisation and structure determination NCAM 1FN3 -- 2FN3 was concentrated to 13 mg/ml in TBS , and crystals were obtained by hanging drop vapour diffusion at room temperature using 2.2 M ammonium sulfate , 0.1 M sodium citrate , pH 5.2 , 0.2 M potassium/sodium tartrate and 3 -- 5 % ethanol as precipitant . Crystals grew within 2 days and belong to space group P212121 with unit cell dimensions a = 52.77 Å , b = 71.35 Å and c = 98.22 Å . There are two 1FN3 -- 2FN3 molecules in the asymmetric unit , resulting in a solvent content of ∼ 38 % . Crystals were flash-frozen in liquid nitrogen after brief soaking in mother liquor supplemented with 20 % glycerol . A crystal was soaked in mother liquor supplemented with 300 mM potassium iodide for 30 s before freezing to obtain a heavy atom derivative . Diffraction data from native and KI derivative crystals were collected at 100 K on station 14.1 at the Synchrotron Radiation Source -LRB- SRS -RRB- Daresbury and on station ID29 at the European Synchrotron Radiation Facility Grenoble , respectively . The NCAM 1FN3 -- 2FN3 M610R mutant was concentrated to 14 mg/ml in TBS , and crystals were obtained by sitting drop vapour diffusion at room temperature using 2 M ammonium sulfate and 0.1 M sodium acetate , pH 4.6 , as precipitant . Crystals grew within 3 -- 4 days and belong to space group P212121 with unit cell dimensions a = 92.74 Å , b = 107.49 Å and c = 161.18 Å . There are six copies of mutant 1FN3 -- 2FN3 in the asymmetric unit , resulting in a solvent content of ∼ 42 % . Crystals were flash-frozen in liquid nitrogen after brief soaking in mother liquor supplemented with 20 % glycerol , and diffraction data were collected at 100 K on station 10.1 at the SRS Daresbury . The diffraction data were processed with MOSFLM ‡ and programs of the CCP4 suite .48 The structure of NCAM 1FN3 -- 2FN3 was solved by single-wavelength anomalous dispersion phasing of a KI-soaked crystal using SHARP -LRB- Globalphasing Ltd. , Cambridge -RRB- in full automatic mode . The structure was rebuilt with O49 and refined with Crystallography & NMR System .50 The structure of the NCAM M610R mutant was solved with some difficulty by molecular replacement with PHASER ,51,52 using the isolated FN3 domains of the NCAM 1FN3 -- 2FN3 structure as search models . Data collection , phasing and refinement statistics are summarised in Table 1 . The figures were made with PyMOL § . SPR experiments Binding experiments were performed on a Biacore 3000 instrument -LRB- GE Healthcare -RRB- at 25 °C . Proteins were immobilised on activated CM4 or CM5 chips using standard amine coupling procedures following the manufacturer 's instructions . Briefly , flow cells were activated with 20 μl of a mixture of 0.2 M 1-ethyl-3 - -LRB- 3-dimethylaminopropyl -RRB- carbodiimide and 0.05 M N-hydroxy-sulfosuccinimide at a flow rate of 5 μl/min . The proteins to be immobilised -LRB- 50 -- 100 μg/ml in 10 mM sodium acetate , pH 4.5 -- 5.5 -RRB- were allowed to pass over activated flow cells to reach ∼ 2000 -- 8000 RU , after which unreacted groups were blocked with 20 μl of 1 M ethanolamine , pH 8.5 . Reference flow cells without protein were treated identically . The chips were equilibrated in 10 mM Hepes , pH 7.4 , 150 mM NaCl , 50 μM ethylenediaminetetraacetic acid and 0.005 % surfactant P20 -LRB- HBS-EP buffer -RRB- , and serial dilutions of analyte proteins in PBS -LRB- our recombinant proteins or FGF1 from PeproTech -RRB- were injected at 20 μl/min for 300 s , followed by 200 s of pure buffer to monitor dissociation . Chips were regenerated using HBS-EP with 1 M glycine -- HCl , pH 3.5 . The sensorgrams were analysed with the BiaEvaluation 4.1 software . Protein Data Bank accession codes Coordinates and structure factors for wild-type and M610R NCAM 1FN3 -- 2FN3 have been deposited in the Protein Data Bank with codes 2vkw and 2vkx , respectively .

O O O O O O O O O O B I O O O O O O O O O O O O O B I O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B I O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B I O O O O O O O O O O O O O O O O O B I O O O O O O O O O O O O O O O O O O O O O O O O O O O B I O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O 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O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B I O O O O O O O O O O O O O O O O O O O O O O O B I O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O 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O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O

cam, cell adhesion molecule
ncam, neural cell adhesion molecule
ig, immunoglobulin
fn3, fibronectin type 3
fgfr, fibroblast growth factor receptor
spr, surface plasmon resonance
ru, resonance units
pbs, phosphate-buffered saline
tbs, tris-buffered saline
crystal packing
protein interaction
x-ray crystallography