Purinergic junctional transmission and propagation of calcium waves in cultured spinal cord microglial networks In order to elucidate the mechanisms of purinergic transmission of calcium -LRB- Ca2 + -RRB- waves between microglial cells , we have employed micro-photolithographic methods to form discrete patterns of microglia that allow quantitative measurements of Ca2 + wave propagation . Microglia were confined to lanes 20 -- 100 wide and Ca2 + waves propagated from a point of mechanical stimulation , with a diminution in amplitude , for about 120 . The number of cells participating in propagation also decreased over this distance . Ca2 + waves could propagate across a cell-free lane from one microglia lane to another if this distance of separation was less than about 60 , indicating that propagation involved diffusion of a chemical transmitter . This transmitter was identified as ATP since all Ca2 + wave propagation was blocked by the purinoceptor antagonist suramin , which blocks P2Y2 and P2Y12 at relatively low concentrations . Antibodies to P2Y12 showed these at very high density compared with P2Y2 , indicating a role for P2Y12 receptors . These observations were quantitatively accounted for by a model in which the main determinants are the diffusion of ATP released from a stimulated microglial cell and differences in the dissociation constant of the purinoceptors on the microglial cells . Introduction Microglia give a calcium -LRB- Ca2 + -RRB- transient in mixed cultures of microglia and astrocytes , following mechanical stimulation of a single astrocyte -LSB- 1 , 2 -RSB- . This transient in the microglia is dependent on the release of ATP by the astrocytes -LSB- 1 -RSB- . However , nothing is known about the mechanism of transmission of a Ca2 + signal between microglial cells that allows for propagation of a Ca2 + wave in populations of microglia -LSB- 3 -RSB- . In the absence of neurons , a Ca2 + wave in astrocytes propagates for hundreds of microns from a point of mechanical stimulation -LRB- e.g. , -LSB- 4 , 5 -RSB- -RRB- . It remains to be seen whether a Ca2 + signal can be transmitted between microglia in such a way that there is propagation of a Ca2 + wave . The Ca2 + waves in astrocytes , when initiated at a point in a culture , can jump cell-free gaps of different widths formed by scraping away cells -LSB- 4 , 6 -RSB- . Such Ca2 + waves propagate across these gaps with a delay that increases with gap width until this width reaches about 150 , when such propagation fails . More recent research has used micropatterned arrays of astrocytes in which lanes of cells about 110 wide alternate with cell-free lanes about 40 wide -LSB- 4 , 5 -RSB- . If a Ca2 + wave is initiated by mechanical stimulation in an astrocyte in just one lane it propagates both along the lane as well as transversely across the cell-free lanes into the adjacent astrocyte lanes with a delay of about 10 s . These observations support the idea that the transmission of Ca2 + waves between astrocytes involves the release of a diffusible substance . The question of whether this wave is transmitted between microglia by a diffusible substance has not been addressed . It is known that microglia collecting in the vicinity of a stab wound express connexin CX43 and that these cells are coupled by such connexins in vitro under the control of certain cytokines -LSB- 7 -RSB- . However , given the relative low density of resting microglia in the brain compared to that of astrocytes -LSB- 8 -- 10 -RSB- , it seems unlikely that connexins are used by microglia in normal circumstances to transmit Ca2 + waves . We have therefore used micropatterned arrays of microglia to ascertain if the Ca2 + wave can propagate across cell-free regions , thus indicating that a diffusible substance is involved in transmission . As shown below , this turns out to be the case . Microglia possess P2X7 , P2Y1 , P2Y2/4 , P2Y6 , P2Y12 , P2Y13 and P2Y14 purinoceptors -LSB- 11 -- 14 -RSB- with P2Y12 receptors and P2X7 receptors unique to microglia , at least in the hippocampus -LSB- 15 -RSB- . There is evidence that some microglia cells possess predominantly P2X receptors and others predominantly P2Y receptors -LSB- 16 -RSB- . P2X7 receptors on microglia are involved in apoptosis , transcription and microvesicle shedding -LSB- 17 -RSB- . Activation of P2X7 receptors can lead to the release of pro-inflammatory cytokines , such as TNFα -LSB- 18 , 19 -RSB- . P2Y1 and P2Y2/4 receptors are involved in the release of the cytokine IL-10 that acts to markedly reduce the release of the pro-inflammatory cytokines -LSB- 20 -RSB- . Most -LRB- 85 % -RRB- resting microglia respond to ATP with a Ca2 + transient -LSB- 21 -RSB- as a consequence of an action of ATP on P2X and P2Y receptors -LSB- 11 , 14 -RSB- . The activation of P2X receptors leads to an influx of calcium ions whereas the activation of P2Y receptors releases calcium from internal stores -LSB- 21 -- 26 -RSB- . Microglia can also release ATP using in part ATP-binding cassette -LRB- ABC -RRB- proteins , such as P-glycoproteins -LRB- mdr 1a and mdr 1b -RRB- and multi-drug resistant associated proteins -LRB- mrp1 and mrp4 ; -LSB- 27 -RSB- -RRB- . Taken together , these observations on the action of ATP on purinergic receptors possessed by resting microglia suggest the hypothesis that the transmission of Ca2 + waves between microglia is due to ATP , and this we have investigated . The experimental work is supplemented by calculations using a theoretical model of extracellular communication in cellular networks , originally developed for astrocytes -LSB- 28 -RSB- , and here modified for microglia . Methods The experimental methods for immunohistochemistry , mechanical stimulation of leading to Ca2 + waves in cells , application of drugs , recording Ca2 + waves and seeding cells into lanes were the same as that previously described -LSB- 5 -RSB- . The purification of microglia started when plated mixed glia culture from Sprague-Dawley rat pup spinal cord formed a confluent monolayer -LRB- usually between 1 and 2 weeks -RRB- . The culture was shaken at 200 rpm for 1 h at 37 °C using rotating shaker -LRB- IKA-Vibrax-VXR -RRB- . During shaking , the astrocytes remained adhered to the poly-D-lysine coating whereas the microglia and oligodendrocytes detached from the astrocyte monolayer . Immediately after shaking , the medium containing the detached cells was transferred to a 15-ml centrifuge tube and centrifuged for 5 min at 500 rpm . The supernatant was discarded and the pellet was resuspended in 1 ml DMEM and triturated . Cell density was adjusted by adding fresh DMEM -LRB- typically 1-2 ml -RRB- after cell trituration and 300 μL of the cell suspension was pipetted onto each coverslip containing previously prepared microchannels and then incubated for 15 min . Incubating DMEM was replaced with fresh DMEM containing 50 % of spinal cord astrocytic conditioned medium -LRB- ACM -RRB- . After 15 min , microglia selectively adhere to the poly-D-lysine coating whereas other cell types that may be present , such as any remaining astrocytes and oligodendrocytes , take a longer period of time to adhere -LSB- 29 -RSB- . The purity of the microglial cultures was greater than 98 % according to live staining of the cells with the microglial marker FITC-IB4 -LRB- Invitrogen -RRB- . Microglia-plated microchannels were incubated in ACM supplemented DMEM for 48 h before use in experiments -LRB- Fig. 1 -RRB- . The medium was removed and replaced daily . Fig. 1The distribution of microglia , immunostained with anti-CSF-1R , in parallel lanes of width 20 and separation 45 ; the calibration bar is 45 For Ca2 + recording , the relative fluorescence amplitude -LRB- ΔF/F -RRB- , was calculated using the formula where F is the fluorescent intensity during the Ca2 + transient , is the intensity averaged over the interval immediately before the calcium transient and is the average fluorescence intensity measured in several cell-free areas . Ca2 + transients with a maximum ΔF/F value less than 0.3 -LRB- being 15 % of the largest Ca2 + transient observed in a lane of microglia -RRB- were discounted as being too close to the noise level to be reliable . All experiments were repeated at least three times and values are presented as mean ± s.d. Statistical significance was determined with the use of unpaired t-tests and ANOVA , and P < 0.05 was considered significant . All P2Y receptor antagonists were obtained from TOCRIS . Mathematical model A detailed description of a mathematical model of purinergic transmission in glial networks was given in -LSB- 28 -RSB- and subsequently applied to experimental results on such transmission between astrocytes -LSB- 5 -RSB- . This model has been adapted to the present case of microglial networks . The basic model is the same , so here we give only a brief summary of the main features , highlighting the changes that have been made . Communication between the model microglia is mediated by ATP diffusing in the extracellular space . This ATP binds reversibly to metabotropic receptors -LRB- P2Y -RRB- on the surface of cells so that the ratio of bound to total receptors is given by where -LSB- ATP -RSB- is the extracellular ATP concentration and KR is the dissociation constant for ATP binding . The usual meaning of KR is the concentration of ATP at which half the total receptors are bound ; however , in the present context KR , as well as being a measure of the affinity of receptor types , also reflects additional variables such as spatial variations in receptor density , since ρ is a measure of the effective activity of ATP as a function of space and time . Thus KR is to be interpreted as an effective , rather than an actual , dissociation constant -LRB- see the section `` Receptors '' in -LSB- 5 -RSB- -RRB- . Each microglial cell is represented by a cube of side 8.3 , and these cubes are arranged in 2D arrays with their centres 25 apart . As explained in -LSB- 28 -RSB- , this simplified geometry does not model the spatial complexity of a real cell , but is a lumped approximation . The Ca2 + wave can be initiated either by increasing the IP3 concentration in a single cell , or by applying ATP extracellularly . In the present calculations , a fixed concentration of ATP -LRB- typically 20 μM -RRB- is applied for an extended time -LRB- typically 5 s -RRB- to the surface of one model microglial cell . The parameters used are those given in Table 1 of -LSB- 28 -RSB- , except that the parameter governing the ATP release rate , VATP , has been reduced from 2 × 10 − 11 to 5 × 10 − 12μmol − 2 s − 1 in order to obtain agreement with the experimental observations . Results Quantitative characteristics of Ca2 + wave propagation The amplitude and velocity of propagating Ca2 + waves in microglia at different positions along microglia lanes , from a point of mechanical initiation in a microglia , were determined . Figures 2A and B show the extent of propagation from the point of stimulation in two lanes , ~ 80 and ~ 40 wide , respectively . The Ca2 + wave propagates with diminution in amplitude -LRB- Fig. 2C -RRB- , at a velocity of about 5 s − 1 , over a distance of at most 120 before becoming undetectable . This velocity is about one-quarter of that for Ca2 + wave propagation in astrocyte lanes -LSB- 5 -RSB- . Quantitation of these observations for four different sets of microglia lanes in four cultures is shown in Fig. 3 . Figure 3a shows that there is a continual decrease in amplitude of the Ca2 + wave over 120 at which distance the amplitude falls below 15 % to 20 % of the initial amplitude and could no longer be reliably detected . The percentage of microglia cells across a lane that gives a Ca2 + peak amplitude change that is greater than 15 % of the peak amplitude at the site of initiation remains high for about the first 80 and then declines rapidly over the succeeding 40 -LRB- Fig. 3b -RRB- . On the other hand , the peak Ca2 + amplitude increases rapidly with an increase in the local density of cells that give an observable Ca2 + transient response -LRB- Fig. 3c -RRB- . Fig. 2The Ca2 + wave propagates along a lane of microglia with decreasing amplitude from the point of initiation . A and B show results for two different lanes of microglia , of widths 82 ± 5 and 38 ± 4 , respectively . In each case , -LRB- a -RRB- shows the site of Ca2 + initiation by mechanical stimulation with a micropipette and -LRB- b -RRB- shows the microglia that responded with a Ca2 + transient -LRB- indicated by open circles -RRB- after stimulation at the site indicated by the arrow ; the calibration bar is 45 . C shows the timecourse of the Ca2 + wave at different distances along a lane from the site of initiation , as indicated . The mechanical stimulus was applied for 1 s starting at time zeroFig . 3Quantitative characteristics of the Ca2 + wave for a number of microglia lanes . a shows the average peak amplitude of Ca2 + deliveries along the lanes before becoming undetectable at about 120 -LRB- values normalized to the peak Ca2 + concentration at the stimulating electrode -RRB- . b shows the number of microglia that give a Ca2 + response at different positions along the length of a lane , expressed as a percentage of the total number of Ca2 + indicator-labeled microglia at that position ; this remains high for about 80 and then declines . c shows that the average amplitude of the peak Ca2 + wave in equal-width segments of a microglia lane increases with the number of microglia that propagate Ca2 + in the segment . Results in a , b and c are for three different lanes in three different cultures . In a and b the distance is from equal-width segments along a lane -LRB- for which the average peak Ca2 + was calculated for all microglia in the segment -RRB- to the site of mechanical initiation of the Ca2 + wave The restricted local propagation of the Ca2 + wave from the point of initiation is emphasized by experiments in which different sites of initiation along a single lane of microglia are determined . As Fig. 2 shows , in each case the Ca2 + wave propagation is restricted to regions of about 100 diameter around the site of initiation . Evidence for release and diffusion of ATP during Ca2 + wave propagation In order to test for the possibility that mechanically stimulated microglial cells release a diffusible substance , parallel lanes of microglia were constructed , separated by cell-free lanes -LRB- see Fig. 1 -RRB- . Tests were then made of the extent to which Ca2 + waves could propagate across these cell-free lanes of widths 70 or more , independent of the width of the microglia lanes -LRB- Figs. 4A and B -RRB- . No such propagation was observed in 20 experiments . On the other hand , if the cell-free lanes were less than ~ 60 wide there was always successful propagation of the Ca2 + wave across the cell-free lanes -LRB- Fig. 4C -RRB- . It appears then that a diffusible substance is released by the excited microglia and is able to initiate Ca2 + transients in them . Fig. 4Propagation of a Ca2 + wave occurs between microglial lanes if these are not separated by distances greater than about 60 . A and B show parallel lanes of microglia in which the lane widths are 75 ± 3 and 25 ± 3 , respectively , separated by cell-free lanes of 165 ± 3 and 70 ± 4 , respectively ; the open circles indicate the microglia that gave a Ca2 + response following mechanical excitation of the microglial cell indicated by the arrow ; there is no propagation of the Ca2 + wave across these lanes . C shows parallel lanes of microglia in which lane widths are 50 ± 6 and the cell-free lane 46 ± 10 ; the open circles indicate that a Ca2 + wave response was able to propagate across cell-free lanes as well as along the lanes . The calibration bar is 45 in A , B and C . The position of the micropipette in C -LRB- a -RRB- is not evident as it is out of focus Since astrocytes use ATP as a chemical transmitter , and it is known that microglia initiate Ca2 + transients in response to ATP , we determined if ATP was likely to be the diffusible substance released by microglia in order to promote Ca2 + wave propagation . First , Ca2 + wave propagation was blocked by the ATP-degrading enzyme apyrase -LRB- grade III , 60 units per mℓ ; Fig. 5 -RRB- . Second , the effects of antagonists to the P2Y class of purinergic receptors on Ca2 + wave propagation were tested . It is known that the pharmacological profile of P2Y receptor activation on spinal cord microglia and the expression of their mRNA clearly favours P2Y12 receptors , followed by P2Y6 and P2Y1 according to -LSB- 12 -RSB- and P2Y2 , P2Y6 , P2Y12 and P2Y14 according to -LSB- 14 -RSB- . Suramin -LRB- 100 μM -RRB- completely blocked all propagation of the Ca2 + wave along microglial lanes -LRB- compare Fig. 6B with 6A -RRB- , indicating that P2Y6 and P2Y14 are not involved , and this was confirmed for P2Y6 using the specific P2Y6 antagonist MRS 2578 -LRB- 30 μM -RRB- . The specific P2Y1 antagonist MRS 2500 -LRB- 100 μM -RRB- did not block the Ca2 + wave . On the other hand , the P2Y12-specific antagonist 2-MeSAMP -LRB- 300 μM -RRB- blocked Ca2 + wave propagation . Any contribution of P2X receptors known to be present on microglial cells , such as P2X4 and P2X7 -LSB- 11 -RSB- , to Ca2 + wave propagation is likely to be minimal given the blocking effects of the P2Y12-specific antagonist 2-MeSAMP . We conclude that Ca2 + wave propagation between microglial cells involves the release of ATP onto at least P2Y12 receptors . Visentin et al. -LSB- 14 -RSB- have also placed emphasis on the role of P2Y12 receptors in calcium signalling . Fig. 5Propagation of Ca2 + waves is blocked by the ATP-degrading enzyme apyrase . A and B show the extent of propagation of Ca2 + , from the point of mechanical stimulation of a microglial cell , to other microglial cells in a lane . In each case , the top panel -LRB- a -RRB- shows the position of the mechanically stimulating micropipette and the lower panel -LRB- b -RRB- shows the microglial cells that gave a Ca2 + signal -LRB- open circles -RRB- in response to mechanical stimulation at the arrow . A is the control and in B apyrase -LRB- 60 units/mℓ ; grade III , Sigma -RRB- was present with only the stimulated cell now giving a Ca2 + transient . The calibration bar is 45Fig . 6Transmission of the Ca2 + wave between microglial cells is chemical due to the release of ATP . A and B show the extent of propagation of Ca2 + from the point of mechanical stimulation of a microglial cell to other microglial cells in a lane . In each case , the top panel -LRB- -LRB- a -RRB- -RRB- shows the position of the mechanically stimulating micropipette and the lower panel -LRB- -LRB- b -RRB- -RRB- the microglial cell -LRB- s -RRB- that gave a Ca2 + signal -LRB- open circles -RRB- in response to mechanical stimulation at the arrow . A is the control and in B suramin -LRB- 100 μM -RRB- was present blocking P2Y receptors and only the stimulated cell responded with a Ca2 + transient . The calibration bar is 40 Density of P2Y receptors on microglia The theoretical requirement that KR take values from 25 to 45 μM may reflect different densities of P2Y receptors on the microglia , since in the present theory KR depends on this density as well as on the dissociation of ATP from the receptors -LRB- see Theory section above , and also the following section -RRB- . Polyclonal antibody labelling of P2Y12 -LRB- Fig. 7a -RRB- receptors indicated that these are localized in clusters of average diameter 0.45 , as are P2Y receptors on astrocytes -LSB- 5 -RSB- and smooth-muscle cells -LSB- 30 -RSB- . The density of P2Y12 receptors , measured over nine microglial cells , varied about four-fold -LRB- Fig. 7b -RRB- . We suggest that P2Y12 receptors mediate the Ca2 + wave propagation . Since in the present model KR values need to vary over at least a two-fold range , it may be that this variability is partly due to differences in P2Y12 receptor density . Fig. 7Density of P2Y receptors on microglia . a Distribution of anti-P2Y12 receptor immunofluorescence on single spinal-cord microglia in lanes ; the shape of the cell is given by the borders of immunohisto chemical staining . b Histogram of the density of and anti-P2Y12 labelled receptors for different microglia . The error bars indicate ± SEM . At least ten areas on each cell were used to determine the P2Y receptor density Modelling the quantitative characteristics of Ca2 + wave propagation amongst microglia The model of purinergic transmission of the Ca2 + wave given in the Methods was used to give a quantitative description for comparison with the experimental results . A lane of microglia five cells wide and 528 long was considered in which the centre-to-centre distance between the microglia was 25 . The whole lane of microglia was placed on a 2D surface 528 by 528 -LRB- Fig. 8 -RRB- . Each row of five cells possessed KR values assigned by random permutations of the values 25 , 30 , 35 , 40 and 45 μM . Note that these are effective KR values that take into account other properties besides dissociation of ATP from P2Y receptors . Activation of a microglial cell in the centre of the lane , by increasing the ATP concentration about the cell to 20 μM for 5 s , generated a Ca2 + wave that propagated with diminution as shown in Fig. 8 . The Ca2 + wave varied in amplitude and velocity , both across the width of the lane as well as along its length -LRB- Fig. 8 -RRB- . Fig. 8Diagrammatic representation of the theoretical spatial and temporal changes in a Ca2 + wave in a lane of microglia five cells wide following excitation of the central microglia , according to the mathematical model . The Ca2 + wave is initiated by a 5-s pulse of ATP of concen tration 20 μM . The vertical bars give Ca2 + in μM at times t = 7.5 , 15 , 22.5 and 30 s , as indicated . KR values range from 25 to 45 μM for different microglia across each lane A quantitative analysis of Ca2 + wave propagation in a lane , such as that shown in Fig. 8 , gives the results summarised in Fig. 9 . The peak amplitude of Ca2 + in each microglial cell of the lane varied significantly both along the length and across the width of the lane -LRB- Fig. 9a -RRB- . Normalizing the Ca2 + to the largest amplitude observed at the site of initiation shows that many of the cells give a Ca2 + amplitude that is less than 15 % of the largest one -LRB- Fig. 9a -RRB- . Using this as a cut-off for the ΔF/F value that would be observed experimentally -LRB- see Methods -RRB- gives a rate of decline of Ca2 + with distance similar to that observed , from 100 % to 20 % over about 120 -LRB- compare Fig. 9a with Fig. 3a -RRB- . The percentage of cells that gives a Ca2 + amplitude greater than 15 % of the largest amplitude at the site of initiation , for different rows of five cells along the length of the lane , remains high for the first 75 and then declines to about 40 % of maximum at 120 . This is a similar pattern of Ca2 + changes to that observed experimentally along a lane of microglia -LRB- compare Fig. 9b with Fig. 3b -RRB- . The average amplitude of the peak Ca2 + across rows of cells in a lane increases with the number of cells that are activated in a row -LRB- Fig. 9c -RRB- . This is also observed experimentally -LRB- compare Fig. 9c with Fig. 3c -RRB- . Fig. 9Theoretical characteristics of a Ca2 + wave in a microglia lane , according to the mathematical model as in Fig. 8 , for quantitative comparison with the observed characteristics -LRB- see Fig. 3 -RRB- . a shows that the predicted peak amplitude of Ca2 + for all cells in a lane , normalized to that at the site of stimulation , declines with distance along the length of the lane until it becomes undetectable at about 120 -LRB- compare to Fig. 3a -RRB- ; the horizontal line indicates the values of the Ca2 + amplitude below which experimental detection is in the noise level -LRB- set at 15 % -RRB- . b shows the predicted number of microglia that give a Ca2 + response greater than 15 % of the maximum value at different positions along the length of a lane expressed as a percentage of the total number of cells at that position ; this is maintained for about 70 and then declines rapidly -LRB- compare to Fig. 3b -RRB- . c shows that the predicted amplitude of the average peak Ca2 + in rows of a microglia lane increases with the number of microglia that propagate Ca2 + in the row -LRB- compare to Fig. 3c -RRB- These theoretical results highlight the fact that Ca2 + wave propagation amongst microglial cells is very limited compared with that amongst astrocytes -LSB- 5 -RSB- . Experimentally , this was highlighted by mechanical stimulation of microglial cells at different well-separated sites along a single microglial lane , showing that Ca2 + wave propagation was restricted to within about 90 of the stimulating micropipette -LRB- see Fig. 10 -RRB- . Such restricted propagation was also observed along the model lane following stimulation at well-separated sites -LRB- compare Fig. 11 with Fig. 10 -RRB- . The clustering of activated cells near the site of initiation of the Ca2 + wave is , in the model , due to the large amount of ATP released in this region . Fig. 10Stimulation of microglia at different sites in a lane leads to Ca2 + wave propagation confined to the vicinity of the stimulating electrode . Shown in -LRB- a -RRB- , -LRB- b -RRB- and -LRB- c -RRB- are three different sites , greater than 140 apart , of mechanical stimulation by a micropipette of a single cell in a single lane of microglia . In -LRB- d -RRB- is shown that the Ca2 + waves initiated in each case -LRB- -LRB- a -RRB- to -LRB- c -RRB- -RRB- are confined to a region within 90 of the stimulating micropipette , with each set of symbols indicating the extent of Ca2 + wave propagation . The calibration bar is 45 . The position of the micropipette in C -LRB- a -RRB- is not evident as it is out of focusFig . 11Theoretical predictions of the distribution of microglia in a lane five cells wide that gave a Ca2 + response , with an amplitude greater than 15 % of maximum , following excitation of a single microglial cell at three different sites along the length of the lane , indicated by filled circles -LRB- ∙ -RRB- . The dots indicate the positions of microglia in the lane . The diamonds -LRB- ⋄ -RRB- indicate microglia that gave a response following stimulation of the microglia near the left-hand end of the lane -LRB- sixth cell from the left at − 250 -RRB- , the open circles -LRB- ∘ -RRB- responses following stimulation of the microglia near the right-hand end of the lane -LRB- sixth cell from the right at 250 -RRB- and the plusses -LRB- + -RRB- microglia in the centre of the lane -LRB- at 0 -RRB- . -LRB- A longer lane -LRB- 800 -RRB- has been used for this calculation . -RRB- The KR values range from 25 to 45 μM for different microglia across each lane . Note that there is no overlap in the Ca2 + wave domains of each stimulated microglial cell , the diamonds , crosses and circles designating discrete regions The model of purinergic transmission of Ca2 + waves was used to see if it could account for Ca2 + propagation along and between lanes of microglia , such as those shown in Fig. 4 . Figure 12 shows the propagation of Ca2 + waves in three such parallel lanes of cells , separated by cell-free lanes 42 wide , following initiation of the Ca2 + wave in the middle row of the middle lane by applying 20 μM of ATP for 5 s at a central cell . There is propagation of the Ca2 + wave over about six cells of the middle lane before the side lanes are engaged , at about 7 s after application of the initiating stimulus -LRB- Fig. 12 -RRB- . Both side lanes first generate a Ca2 + wave that is in cells in a row opposite or nearly opposite the row containing the initiating cell in the middle lane . By 13 s the crest of the Ca2 + wave has reached the limits of Ca2 + propagation at about 100 from the site of initiation , by which time it has travelled less than 75 % of that distance along adjacent lanes -LRB- Fig. 12 -RRB- . Very few cells are engaged in Ca2 + wave propagation in these adjacent lanes and propagation fails over distances of about 70 along the lane and 50 across it . Fig. 12A diagrammatic representation of the theoretical spatial and temporal changes in Ca2 + in three lanes of microglia each five cells wide , separated by cell-free lanes 42 wide , following excitation of a single microglial cell in the middle row of the middle lane . The Ca2 + wave is initiated by a 5-s pulse of ATP of concentration 20 μM on the central microglial cell at time t = 0 . The vertical bars give Ca2 + in μM at times t = 7.5 , 15 , 22.5 and 30 s , as indicated . KR values range from 25 to 45 μM for different microglia across each lane . Note the limited propagation of the Ca2 + wave in both the middle and side lanes The question arises as to whether regeneration of ATP in each cell is necessary . Repeating calculations with regenerative release switched off , and thus only pure diffusion of ATP from its initial release site , gave travel distances along lanes reduced by about 20 % . We investigated whether this reduced distance could be compensated for by increasing the initial release of ATP , but this then recruited cells in adjacent lanes in a way not observed experimentally . Thus the theoretical calculations indicate a role for intracellular regeneration of ATP , but at a much lower rate than in astrocytes . Discussion Although activation of P2X7 receptors by ATP leads to an influx of calcium ions in microglial cells -LSB- 16 , 31 -- 33 -RSB- it does not seem that these receptors mediate the Ca2 + wave propagation in these cells as this is blocked by suramin which does not block P2X7 receptors . It seems likely that P2X7 receptors are activated at higher concentrations of ATP than is required for P2Y receptor activation -LSB- 33 -RSB- , suggesting that the concentration of ATP reached at the receptors after its release from microglia is insufficient to excite P2X7 receptors . ATP acts on microglial cells to both release calcium from internal stores and to evoke an influx of calcium -LSB- 34 , 35 -RSB- . P2Y receptors mediate Ca2 + release from internal stores in microglial cells -LSB- 14 , 21 , 32 , 33 , 36 , 37 -RSB- , the extent of this release being under the modulatory control of P2X receptors -LSB- 25 , 32 -RSB- and of toll-like receptors -LSB- 38 -RSB- . ATP released following stimulation of astrocytes can generate Ca2 + transients in nearby microglial cells -LSB- 2 -RSB- . Repeated stimulation of the astrocytes releases sufficient ATP to activate P2X7 receptors on the microglial cells , greatly increasing membrane permeability in the microglial cells -LSB- 1 -RSB- and leading to the release of inflammatory cytokines , such as IL-2 , from the cells -LSB- 39 -RSB- . The present work suggests that in these experiments the ATP released in moderate amounts from singly stimulated astrocytes most likely acts on P2Y12 receptors on microglial cells . We could block the ATP-dependent Ca2 + propagation in these cells with suramin , which blocks P2Y1 , P2Y2 and P2Y12 receptors , but could not be blocked by P2Y1 receptor antagonists . Given that the predominant receptor on microglial spinal cord cells shows the pharmacological profile of P2Y12 -LSB- 12 -RSB- -LRB- see also -LSB- 14 -RSB- -RRB- , we conclude that this receptor , which we found in relatively high density , is most likely to be mediating the effect of ATP . Thus there is no evidence that P2X7 receptors are engaged in Ca2 + propagation in microglia . It is interesting to note in this regard that microglial cells rapidly re-orientate their processes towards a site of ATP release in vivo as a consequence of the action of the released ATP on P2Y receptors , an action that is blocked by apyrase -LSB- 40 -RSB- and that nucleotides acting on P2Y12 receptors of microglia exert a chemotactic effect -LSB- 41 -RSB- . We have used soft lithography techniques of micro-fabrication to allow controlled and discrete patterning of microglia so that quantitative investigations can be carried out -LSB- 42 -RSB- . Such an approach avoids the difficulties inherent in the random seeding of microglial cells on a homogeneous substrate for carrying out quantitative measurements of the properties of propagating calcium waves -LSB- 43 -RSB- . The technique allows lanes of microglial cells with controlled widths of from 15 to over 150 , separated by cell-free regions with the same range of widths -LSB- 44 -RSB- . It is unlikely that this fabrication method affects the seeded microglial cells , since when applied to astrocytes the rates of propagation of the Ca2 + waves remain about the same as determined in random seeded cultures -LSB- 4 , 5 -RSB- . The amplitude of the Ca2 + wave , and the percentage of microglial cells excited to give a Ca2 + response , were correlated along the length of a microglial lane , both decreasing from near the site of initiation over a distance of about 120 before the wave ceased to be detectable . Decreases in amplitude of Ca2 + waves from a site of mechanical initiation have often been observed in randomly seeded astrocytes over a homogeneous substrate , but in this case over several hundred microns -LSB- 45 -- 47 -RSB- . The purinergic gliotransmission model suggests that the decline in amplitude of the Ca2 + wave is due to the relatively high level of ATP released from the stimulated microglial cell , which then dominates the concentration profile of ATP within approximately 100 . The decline in amplitude of the wave approximately follows this concentration gradient of ATP . The decline in the percentage of microglial cells excited to give a Ca2 + response within this 100 range is then attributed to a failure of microglial cells possessing a relatively high KR to generate a Ca2 + response as the concentration of ATP declines over the same range . The more than two-fold range in the KR values used in our model is comparable to the range of P2Y12 receptor densities found in microglial cells using immunohistochemistry . Our work shows that there can be propagation of Ca2 + waves between parallel lanes of microglial cells when these are about 30 wide and separated by cell-free lanes about 40 wide , but this does not occur until many microglial cells in the initiating lane undergo a Ca2 + response . It seems likely that a certain minimum amount of ATP must be released from the initiating lane , involving a certain minimum number of microglial cells undergoing a Ca2 + response , before nearby microglial lanes are excited . Since ATP can diffuse across cell-free lanes as wide as 150 from astrocyte-seeded lanes -LSB- 5 -RSB- , it seems likely that the ATP released from microglial cells is much less than that from astrocytes since the P2Y12 receptors in the model for microglia possessed lower KR values -LRB- 25 − 45 μM -RRB- than in the model for astrocytes -LRB- 25 − 125 μM ; -LSB- 5 -RSB- -RRB- . The purinergic transmission model can account for the observed extent of the Ca2 + wave provided pure diffusion of ATP from the stimulation site is supplemented by regenerative release of ATP from the microglia . Fluctuations in the density of excited microglia along a lane leads to local fluctuations in the amplitude of the Ca2 + wave due to changes in the local ATP concentration , consequential on the changes in the local number of microglial cells excited . This is modelled by assigning a range of KR values to the P2Y receptors on the microglia , and this also reflects the density of receptors on individual microglial cells . Stimulation of three different microglial cells some hundreds of microns apart gave a Ca2 + response in microglia at highest density closest to the site of stimulation . Our model quantitatively explains these observations as arising from the large amount of ATP released by the stimulated microglial cell diffusing to activate microglia within about 100 .

O O B O B O B I O O O O O O O O O O O O O O B O O O O O O O O O O O O O O O O O O O O O B O O O O O O O O B O B O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B O O O O O O O O O O O O O O O O O B O O O O O O O O O O O O O O O O B O O O O O O O O O O O O O O O O O O B O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B O O O O O O O O O O O O B O O O O O O O O O O O O O O O O O O O O B O O O O O O O O O O O O O O O O O O O O O O O B O O O O O O O O O O O B O O O O O O O O B O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B O O O O O O O B O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B O O O O O O O O O O O O O O O O O O O O O O O B O O O O O O O O O O O O O B O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B O O O O O O O O O O O O O O O O O O O O O O B O O O O O O O O O O O O O O O O B O O O O O O O O O O O O O O O O O O O O O O B O O O O O O O O O O O O O B O O O O O O O O O O O O O O O O O O O O O O O O O O O O B O O O O O O O O O O O O O O O B O O O O O O O O O O O O O O O O O B O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B O O O O O B O O O O O B O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B O O O O O O O O O O O O O O O O O B O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B O O O O O O O O O O B O O O O B O O O O O O O O O O B O O O O O O O O O O O O B O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B O O O O O O O O O O O O O O O O B O O O O O O O O O O O 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O O O O O O O B O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B O O O O O O O O O O O B O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B O O O O O O O O O O B O O O O O O O O B O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B O O O O O O O O O O O O O O O O O O O O O O B O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B O O O O O O O O O O O O O O O B O O O O O B O O O O O O O O O O O O O B O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B 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O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B O O O O O O O B O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O B O O O O

calcium waves
microglia
propagation
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