Patent ID: 12256707

DETAILED DESCRIPTION OF THE EMBODIMENTS

Example

A breeding method of a superior long-staple cotton variety allowing spinning 360 N ultra-fine and high-grade combed yarns was provided, including the following steps:G. barbadenseXinhai 21 was treated for 154 h under a negative vacuum pressure of −0.32 MPa and then sown in a field, M1 and M2 populations were subjected to sealing-flower selfing, and a high-quality and high-verticillium wilt-resistance individual BMC1563 was screened out; upland. cotton Xinluzhong 14 was sown in an artificial blight and verticillium wilt mixed severe disease nursery, a disease-resistant individual was screened out, a stem apex of the disease-resistant individual was cultivated on aF. oxysporum-containing modified MS medium in a greenhouse, and after seedling establishment, a seedling was transplanted in a field, and a high-blight and verticillium wilt-resistance individual Xinluzhong 14 was screened out; the BMC1563 as a female parent was crossed with the high-blight and verticillium wilt-resistance individual Xinluzhong 14 as a male parent to obtain an F1hybrid, and the F1hybrid as a female parent was backcrossed with the BMC1563 as a male parent for three generations to obtain BC3F1; the BC3F1was selfed for 4 generations to obtain BMC18394; and Xinhai 40 as a female parent was crossed with the BMC18394 as a male parent to obtain a first-generation hybrid, and the first-generation hybrid was subjected to sealing-flower selling for 6 generations to finally obtain a long-staple cotton variety BMC79. During the selective breeding, F2, F4, and F6-generation populations were subjected to weighted balancing selection of fiber quality, that is, an individual or line BMC79 with a fiber length and a fiber strength greater than an average fiber length and an average fiber strength of a population and with a fiber micronaire value and a fiber diameter smaller than an average fiber micronaire value and an average fiber diameter of the population was screened out. The variety had a seed cotton yield per unit of 362.00 kg/mu, and a fiber of the long-staple cotton variety had an average upper-half length of 38.88 mm, a uniformity index of 90.35%, a specific strength at break of 58.50 cN/tex, a micronaire value of 3.23, and a diameter of 9.97 μm.

Application Example

In the winter of 2003, in Sanya of Hainan, a high-quality and high-verticillium wilt-resistance long-staple cotton variety BMC1563 as a female parent was crossed with an upland cotton variety Xinluzhong 14 as a male parent.

In 2004, in Korla of Xinjiang, F1(BMC1563X Xinluzhong 14) as a female parent was backcrossed with BMC1563 as a male parent, and seeds of the backcrossing were harvested together.

In the winter of 2004, in Sanya of Hainan, BC1F1(BMC1563X Xinluzhong 14) as a female parent was backcrossed with BMC1563 as a male parent, and seeds of the backcrossing were collected separately and subjected to fiber detection; and individuals with an upper-half fiber length of less than or equal to 37.00 mm, a specific strength at break of less than or equal to 50.00 cN/tex, a micronaire value of less than 3.00, or a micronaire value of more than 3.60 were eliminated.

In 2005, in Korla of Xinjiang, BC2F1(BMC1563X Xinluzhong 14) as a female parent was backcrossed with BMC1563 as a male parent, and seeds of the backcrossing were harvested. separately and subjected to fiber detection; and individuals were compared in terms of traits such as plant type, fiber quality, single-plant boll formation, single-boll weight, lint percentage, and fruit-bearing shoot nodes to obtain a BMC3F1seed (BMC1563X Xinluzhong 14) with excellent comprehensive traits.

In the winter of 2005, in Sanya of Hainan, the BMC3F 1 (BMC1563X Xinluzhong 14) seed was sown and subjected to sealing-flower selfing; and seeds of the selfing were collected separately and subjected to fiber detection; and individuals with an upper-half fiber length of less than or equal to 37.00 mm, a specific strength at break of less than or equal to 50.00 cN/tex, a micronaire value of less than 3.00, or a micronaire value of more than 3.60 were eliminated.

In 2006, in Korla of Xinjiang, the BMC3F2(BMC1563X Xinluzhong 14) seed was sown, non-disease-resistant individuals were eliminated, and disease-resistant individuals were subjected to sealing-flower selfing; seeds of the selfing were collected separately and subjected. to fiber detection; and individuals with an upper-half fiber length of less than or equal to 37.00 mm, a specific strength at break of less than or equal to 50.00 cN/tex, a micronaire value of less than 3.00, or a micronaire value of more than 3.60 were eliminated.

In 2007, in Korla of Xinjiang, the BMC3F3(BMC1563X Xinluzhong, 14) seed was sown, non-disease-resistant individuals were eliminated, and disease-resistant individuals were subjected to sealing-flower selfing; seeds of the selfing were collected separately and subjected to fiber detection; and individuals with an upper-half fiber length of less than or equal to 37.00 mm, a specific strength at break of less than or equal to 50.00 cN/tex, a micronaire value of less than 3.00, or a micronaire value of more than 3.60 were eliminated.

In 2008, in Aksu of Xinjiang, the BMC3F4(BMC1563X Xinluzhong, 14) seed was sown, non-disease-resistant individuals were eliminated, and disease-resistant individuals were subjected to sealing-flower selling; seeds of the selling were collected separately and subjected to fiber detection; and individuals with an upper-half fiber length of less than or equal to 37.00 mm, a specific strength at break of less than or equal to 50,00 cN/tex, a micronaire value of less than 3.00, or a micronaire value of more than 3.60 were eliminated.

In 2009, in Aksu of Xinjiang, the BMC3F5, (BMC1563X Xinluzhong 14) seed was sown, non-disease-resistant individuals were eliminated, and disease-resistant individuals were subjected to sealing-flower selfing; seeds of the selfing were collected separately and subjected. to fiber detection; and individuals with an upper-half fiber length of less than or equal to 37,00 mm, a specific strength at break of less than or equal to 50.00 cN/tex, a micronaire value of less than 3.00, or a micronaire value of more than 3.60 were eliminated, and an individual with blight and verticillium wilt resistance and high fiber quality was screened out and named BMC18394.

In 2010, in Aksu of Xinjiang, a long-staple cotton variety Xinhai 40 with high quality and verticillium wilt resistance as a female parent was crossed with the long-staple cotton variety BMC18394 with blight and verticillium wilt resistance and high fiber quality as a male parent.

In the winter of 2010, in Sanya of Hainan, F1(Xinhai 40XBMC18394) was sown and subjected to sealing-flower selfing; and seeds of the selfing were collected together.

In 2011, in Aksu, Aral, Korla, or the like of Xinjiang, F2(Xinhai 40XBMC18394) was sown and subjected to sealing-flower selfing, low-generation large populations were subjected to multi-ecological selection, and seeds were harvested separately and subjected to fiber detection and weighted balancing selection of fiber quality; and individuals with an upper-half fiber length of less than or equal to 37.00 mm, a specific strength at break of less than or equal to 50.00 cN/tex, a micronaire value of less than 3.00, or a micronaire value of more than 3.60 were eliminated.

In the winter of 2011, in Sanya of Hainan, F3(Xinhai 40XBMC18394) was sown and subjected to sealing-flower selfing; seeds of the selfing were collected separately and subjected to fiber detection; and individuals with an upper-half fiber length of less than or equal to 37.00 mm, a specific strength at break of less than or equal to 50.00 cN/tex, a micronaire value of less than 3.00, or a micronaire value of more than 3.60 were eliminated.

In 2012, in Aksu, Aral, Korla, or the like of Xinjiang, F4(Xinhai 40XBMC18394) was sown and subjected to sealing-flower selfing, low-generation large populations were subjected to multi-ecological selection and weighted balancing selection of fiber quality, and seeds were harvested separately and subjected to fiber detection; and individuals with an upper-half fiber length of less than or equal to 37.00 mm, a specific strength at break of less than or equal to 50.00 cN/tex, a micronaire value of less than 3.00, or a micronaire value of more than 3.60 were eliminated.

In 2013, in Aksu of Xinjiang, F5(Xinhai 40XBMC18394) was sown in a blight and verticillium wilt mixed disease nursery; disease-resistant lines were screened out, and non-disease-resistant lines were eliminated; 20 individuals were randomly selected from each disease-resistant line and subjected to fiber detection; and lines with an upper-half fiber length of less than or equal to 37.00 mm, a specific strength at break of less than or equal to 50.00 cN/tex, a micronaire value of less than 3.00, or a micronaire value of more than 3.60 were eliminated.

In 2014, in Aksu of Xinjiang, F6(Xinhai 40XBMC18394) was sown in a blight and verticillium wilt mixed disease nursery; disease-resistant lines were screened out, and non-disease-resistant lines were eliminated; 20 individuals were randomly selected from each disease-resistant line and subjected to fiber detection; and lines with an upper-half fiber length of less than or equal to 37.00 mm, a specific strength at break of less than or equal to 50.00 cN/tex, a micronaire value of less than 3.10, or a micronaire value of more than 3.60 were eliminated to obtain an excellent line.

In 2015, in Aksu of Xinjiang, F7(Xinhai 40XBMC18394) was sown, excellent lines were subjected to multi-repeat and line-comparison tests; non-disease-resistant lines were eliminated, and a disease-resistant line was subjected to sealing-flower selfing; and 20 individuals were randomly selected from each line and subjected to fiber detection.

In 2016, in Aksu of Xinjiang, selected lines were sown and subjected to multi-repeat and line-comparison tests. The lines were compared in terms of traits such as plant type, fiber quality, single-plant boll formation, lint percentage, single-boll weight, and disease resistance to obtain a genetically-stable line with prominent comprehensive traits of Xinhai 40 X [(high-quality and high-verticillium wilt-resistance variety BMC 1563 X high-blight and verticillium wilt-resistance variety Xinluzhong 14) BMC18394], which was named BMC79. A fiber quality of the BMC79 was detected by the Cotton Quality Supervision, Inspection, and Test Center of the Ministry of Agriculture according to the GB/T 20392-2006“Test Method of Properties of Cotton Fibers by High Volume Instruments”, and results were as follows (Table 1): the fiber had an average upper-half length of 38.88 mm, a uniformity index of 90.35%, a specific strength at break of 58.50 cN/tex, a micronaire value of 3.23, and a diameter of 9.97 μm. According to trial spinning results of China Resources Textile Co., Ltd., the fiber could be used to spin 300 N to 360 N extra-high-count yarns, and a strength of COMNE300 produced by the fiber was 14.5% higher than a strength of the optimal Egyptian long-staple cotton Giza 45 (G45) worldwide.

TABLE 1Test results of cotton fibers by the Cotton Quality Supervision,Inspection, and Test Center of the Ministry of Agriculture of ChinaTest time: January 2018AverageSpinningVarietySamplingupper-halfUniformitySpecific strengthMicronaireuniformitynamesitelength/mmindex/%at break/cN · tex−1valueDiameter/μmindexBMC79Korla Aral38.8890.3558.503.239.97251.17Giza 45Korla Aral34.6085.6042.803.2410.20184.00Notes:1. Testing basis: GB/T20392-2006 “Test Method of Properties of Cotton Fibers by High Volume Instruments”.2. Main instrument: large-capacity fiber detector (ZXYQ 09-1).

TABLE 2The genetic resource BMC18394 has the following depositoryinformation at the China Center for Type Culture Collection(CCTCC) Depository.Genetic resourceDespot informationBMC18394CCTCC Accession No .: P202406Date of deposit: Mar. 6, 2024Culture name: G. barbadense L. seed BMC18394;Gossypium barbadense Linn.Depository: China Center for Type CultureCollection (CCTCC) Wuhan University, No. 299Bayi Road, Wuchang District, Wuhan City, HubeiProvince, China.

TABLE 3The genetic resource BMC18394, CCTCC Accession No.: P202406,has the following morphological and physiological traits:Species:GossypiumbarbadenseL.:G.barbadenseL. seed BMC18394;Gossypium barbadense Linn.(CCTCC Accession No.: P202406)Areas of AdaptationXinjiang, China and similar areasof the world suitable for islandcotton cultivation.ApplicantHualan Road, Xinxiang, Henan,China Henan Institute Science andTechnologyGeneralPlant HabitIntermediate. Plant type tube type,Compact, Thick stalks.FoliageDense. Cotyledon: kidney shaped.Stem LodgingStems erect.Fruiting BranchNo branchesGrowthIntermediateLeaf ColorDark GreenBoll ShapeLength more than width. Boll ovoid,sharp.Boll BreadthBroadest at baseMaturityDate of 50% open bolls140daysPlant1st Fruiting Branch (cm from7.3cotyledonary node)No. of Nodes to 1st Fruiting2.5Branch (excluding cotyledonarynode)Mature Plant Height in cm (from95.0cmcotyledonary node to terminal)Leaf (Upper most,fully expanded leaf)TypeNORMAL Leaves 5-lobed, Leaveslarger, deep crack, The leaves aredark green.PubescenceMedium. (The leaf blade has fluff)NectariesPresentGlandsLeafPresentStemPresentCalyx LobePresentFlowerPetalsYellowPollenGolden yellowPetal SpotPresent. The petals have erythemaat the base.Seed12.2gSeed Index (g/100, fuzzy basis)BollLint percent (%) Picked32.5%~34.0%Number of Seeds per Boll20.3Boll weight3.4gNumber of Locules per Boll3 To 4 (Many with 3)Boll TypeOPEN (Bell ovoid)Fiber PropertiesSpecify Method (HVI or other)HVICC standard (HVICC)Length (mm/in, 2.5 SL)1.48 in/37.5 mmUniformity (%)89.6Strength Tl (cN/tex)52.3 cN/texElongation E1 (%)6.9%Micronaire (x)3.5Diseases and PestsFusarium WiltHigh resistance to fusarium wilt,the disease index was 3.7.Verticillium WiltHigh resistance to verticillium wilt.The disease index was 5.5.Bacterial BlightGood resistanceRoot-Knot NematodeUnknownBoll WeevilUnknownBollwormModerately resistantReniform NematodeUnknownLygusModerately resistantPink BollwormResistantTobacco Bud WormResistant