Patent ID: 12195473

METHODS OF PREPARATION

Compounds of the present invention can be synthesized from commercially available reagents using the synthetic methods described herein. The examples which outline specific synthetic route below are meant to provide guidance to the ordinarily skilled synthetic chemist, who will readily appreciate that the solvent, concentration, reagent, protecting group, order of synthetic steps, time, temperature, and the like can be modified as necessary, well within the skill and judgment of the ordinarily skilled artisan.

The following Examples are provided to better illustrate the present invention. All parts and percentages are by weight and all temperatures are degrees Celsius, unless explicitly stated otherwise. The following abbreviations in Table 1 have been used in the examples:

TABLE 1MeOHMethanolEtOHEthanolDCMDichloromethaneTEATriethylamineTFATrifluoroacetic acidDMFN,N-DimethylformamideDMAN,N-dimethylacetamideTHFTetrahydrofuranMeCN/ACNAcetonitrileHATU2-(7-Azabenzotriazol-1-yl)-N,N,N′,N′-tetramethyluronium hexafluorophosphateEDCI1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochlorideHOBT1-HydroxybenzotriazoleLiHMDSLithium HexamethyldisilazideHunig's base/DIEA/DIPEAN,N-DiisopropylethylamineEAEthyl acetateminMinute(s)hHour(s)Pre-TLCPreparative thin layer chromatographyprep-HPLCPreparative High Performance Liquid ChromatographySFCSupercritical fluid chromatographyPd(dppf)Cl2[1,1′-Bis(diphenylphosphino)ferrocene]dichloropalladium(II)R.T./r.t.Room temperature(20° C.~30° C.)AcOHAcetic acidPd(PPh3)4Tetrakis(triphenylphosphine)palladiumNCSN-ChlorosuccinimideHexn-HexanePPTSPyridinium 4-toluenesulfonateIPAIsopropanolDHP3,4-Dihydro-2H-pyrana.q./aqAqueousAcOK/KOAcPotassium acetateNMPMethyl-2-pyrrolidinone

Example 1

4-(4-acryloylpiperazin-1-yl)-7-(2-amino-3,4,5,6-tetrafluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Compound 1”); (P)-4-(4-acryloylpiperazin-1-yl)-7-(2-amino-3,4,5,6-tetrafluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Compound 1-1”); and (M)-4-(4-acryloylpiperazin-1-yl)-7-(2-amino-3,4,5,6-tetrafluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Compound 1-2”)

Step 1. 4-methyl-2-(prop-1-en-2-yl)pyridin-3-amine

Into a 350-mL sealed tube purged and maintained with an inert atmosphere of nitrogen, was placed 2-bromo-4-methylpyridin-3-amine (BD, APL099) (15.01 g, 80.25 mmol), 4,4,5,5-tetramethyl-2-(prop-1-en-2-yl)-1,3,2-dioxaborolane (BD, AQA827) (13.62 g, 81.05 mmol), Pd(dppf)Cl2(5.95 g, 8.03 mmol), K2CO3(33.52 g, 240 mmol), dioxane (150 mL) and water (20 mL). The reaction mixture was stirred at 100° C. for 8 h. The reaction mixture was filtered and concentrated under vacuum. The residue was applied onto a silica gel column eluted with EA/hexane (v:v=2:3). This resulted in 11.2 g (94%) of 4-methyl-2-(prop-1-en-2-yl)pyridin-3-amine as yellow oil. LCMS: m/z=149 [M+1]+.

Step 2. 2-isopropyl-4-methylpyridin-3-amine (Intermediate A)

Into a 250-mL round-bottom flask purged and maintained with an inert atmosphere of nitrogen, was placed 4-methyl-2-(prop-1-en-2-yl)pyridin-3-amine (11.2 g, 75.67 mmol) and MeOH (100 mL). Palladium on carbon (2.81 g) was added in three portions. The mixture was degassed under vacuum and then purged with H2(gas) for three cycles. The mixture was stirred for 3 h at 25° C. The resulting mixture was filtered, the filtrate was concentrated under vacuum. This resulted in 11 g (crude) of 2-isopropyl-4-methylpyridin-3-amine which was used directly in the next step. LCMS: m/z=151 [M+1]+.

Step 3. 2-cyano-N-(2-isopropyl-4-methylpyridin-3-yl)acetamide

Into a 100-mL round-bottom flask purged and maintained with an inert atmosphere of nitrogen, was placed 2-cyanoacetic acid (3 g, 35.27 mmol) and DCM (40 mL). Oxalyl chloride (6.2 g, 48.85 mmol) was added in dropwise. After the addition, DMF (0.1 mL) was added. The mixture was stirred for 3 h at 25° C. The resulting solution was concentrated under vacuum. This resulted in 3.10 g (crude) of 2-cyanoacetylchloride which was used directly in the next step.

Into a 100-mL round-bottom flask purged and maintained with an inert atmosphere of nitrogen, was placed 2-isopropyl-4-methylpyridin-3-amine (2.00 g, 13.31 mmol), TEA (5.40 g, 53.36 mmol) and DCM (40 mL) and stirred. The mixture was cooled to 0° C. and then 2-cyanoacetylchloride (3.10 g, crude) was added in dropwise. The resulting solution was stirred for further 2 h at room temperature. The reaction was then quenched by the addition of water (100 mL). The resulting solution was extracted with dichloromethane (3×50 mL), the organic layers were combined and washed with brine (50 mL), dried over anhydrous Na2SO4and filtered, the filtrate was concentrated under vacuum and applied onto a silica gel column eluted with EA/hexane (v:v=3:2). This resulted in 1.00 g (34%) of 2-cyano-N-(2-isopropyl-4-methyl pyridin-3-yl)acetamide as yellow solid. LCMS: m/z=218 [M+1]+.

Step 4. 2-cyano-N-(2-isopropyl-4-methylpyridin-3-yl)-3-oxo-3-(2,5,6-trichloropyridin-3-yl) propanamide

Into a 100-mL round-bottom flask purged and maintained with an inert atmosphere of nitrogen, was placed 2,5,6-trichloronicotinic acid (5.01 g, 22.12 mmol) and SOCl2(30 mL). The mixture was heated to 80° C. and stirred for 2 h. The solution was concentrated under vacuum. This resulted in 5.10 g (crude) of 2,5,6-trichloronicotinoyl chloride which was used directly in the next step.

Into a 250-mL round-bottom flask, was placed 2-cyano-N-(2-isopropyl-4-methylpyridin-3-yl)acetamide (3.01 g, 13.85 mmol) and THF (40 mL). The mixture was stirred at 0° C. NaH (1.16 g, 28.99 mmol) was added in three batches. The mixture was stirred at 0° C. for further 40 min. Then 2,5,6-trichloronicotinoyl chloride (3.19 g, 13.03 mmol) in THF (10 mL) was added in dropwise. The reaction mixture was stirred at 25° C. for 2 h. The reaction mixture was concentrated under vacuum. The resulting crude product was further purified by C18column eluted with ACN/H2O (v/v=1/3). This resulted in 5.89 g (crude) of 2-cyano-N-(2-isopropyl-4-methylpyridin-3-yl)-3-oxo-3-(2,5,6-trichloropyridin-3-yl)propanamide as yellow solid. LCMS: m/z=425 [M+1]+.

Step 5. 6,7-dichloro-4-hydroxy-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (Intermediate B)

Into a 250-mL round-bottom flask purged and maintained with an inert atmosphere of nitrogen, was placed 2-cyano-N-(2-isopropyl-4-methylpyridin-3-yl)-3-oxo-3-(2,5,6-trichloropyridin-3-yl)propanamide (5.89 g, 13.83 mmol) and THF (70 mL) and stirred at room temperature. NaH (2.73 g, 68.25 mmol) was added in batch-wise. The mixture was stirred at 50° C. for 2 h. The reaction mixture was concentrated under vacuum. The residue was dissolved in 100 mL water and adjusted pH to 7 with AcOH. The resulting solid was filtered and dried under vacuum to provide 5.85 g (108% in two steps) of 6,7-dichloro-4-hydroxy-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-car bonitrile as yellow solid. LCMS: m/z=389 [M+1]+.

1H NMR (400 MHz, DMSO-d6) δ 8.77 (d, J=5.7 Hz, 1H), 8.38 (s, 1H), 7.89 (d, J=5.4 Hz, 1H), 3.01-2.88 (m, 1H), 2.19 (s, 3H), 1.21 (d, J=6.9 Hz, 3H), 1.14 (d, J=6.9 Hz, 3H).

The mixture of 6,7-dichloro-4-hydroxy-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile atropisomers (10.59 g, “Intermediate B”) was purified by Chiral-Prep-HPLC with the following conditions: Column, CHIRALPAK IC, 3.0×100 mm, 3 μm; mobile phase, IPA/ACN=(v/v=1/1); detection wavelength, UV 210 nm. This resulted in 4.99 g (47%) of 6,7-dichloro-4-hydroxy-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-car bonitrile (the first eluting isomer, “Intermediate B-1”, M or P atropisomer) as a brown solid;

1H NMR (400 MHz, CD3OD) δ 8.48 (s, 1H), 8.44 (d, J=5.0 Hz, 1H), 7.33-7.22 (m, 1H), 2.76-2.61 (m, 1H), 2.03 (s, 3H), 1.19 (d, J=6.8 Hz, 3H), 1.06 (d, J=6.8 Hz, 3H).

And 4.60 g (43%) of 6,7-dichloro-4-hydroxy-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (the second eluting isomer, “Intermediate B-2”, P or M atropisomer) as a brown solid;

1H NMR (400 MHz, CD3OD) δ 8.49 (s, 1H), 8.44 (d, J=5.0 Hz, 1H), 7.28 (d, J=5.0 Hz, 1H), 2.76-2.63 (m, 1H), 2.03 (s, 3H), 1.19 (d, J=6.8 Hz, 3H), 1.08-1.00 (m, 3H).

Step 6. 4,6,7-trichloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile

Into a 50-mL round-bottom flask purged and maintained with an inert atmosphere of nitrogen, was placed 6,7-dichloro-4-hydroxy-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-car bonitrile (Intermediate B, 980 mg, 2.51 mmol), POCl3(1150 mg, 7.50 mmol), DIEA (1.32 g, 10.21 mmol) and acetonitrile (12 mL). The mixture was stirred at 80° C. for 2 h. The reaction mixture was cooled to room temperature and concentrated under vacuum. This resulted in crude 4,6,7-trichloro-1-(2-isopropyl-4-methyl pyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile which was used directly in the next step.

Step 7. tert-butyl 4-(6,7-dichloro-3-cyano-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridin-4-yl)piperazine-1-carboxylate

Into a 100-mL round-bottom flask purged and maintained with an inert atmosphere of nitrogen, was placed 4,6,7-trichloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (1.20 g, crude) and acetonitrile (20 mL). DIEA (660 mg, 5.10 mmol) and tert-butyl piperazine-1-carboxylate (0.57 g, 3.06 mmol) were added. The reaction mixture was stirred for 2 h at room temperature. The reaction was then quenched by the addition of water (50 mL). The resulting solution was extracted with ethyl acetate (3×50 mL), the organic layers were combined and washed with brine (50 mL), dried over anhydrous Na2SO4, filtered and concentrated under vacuum. The residue was purified by silica gel column eluted with EA/hexane (v/v=30%˜70%). This resulted in 0.92 g (65% in two steps) of tert-butyl 4-(6,7-dichloro-3-cyano-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridin-4-yl)piperazine-1-carboxylate as yellow solid. LCMS: m/z=557 [M+1]+.

Step 8. 4-(4-acryloylpiperazin-1-yl)-6,7-dichloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (Intermediate C)

Into a 20-mL sealed tube purged and maintained with an inert atmosphere of nitrogen, was placed tert-butyl 4-(6,7-dichloro-3-cyano-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridin-4-yl)piperazine-1-carboxylate (920 mg, 1.65 mmol), TFA (4 ml) and DCM (15 mL). The reaction mixture was stirred at room temperature for 2 h. The reaction mixture was concentrated under vacuum. The residue was dissolved by DCM (15 mL) in 50-mL round-bottom flask. DIEA (1.02 g, 10.08 mmol) was added. The reaction mixture was cooled to 0° C. and acryloyl chloride (190 mg, 2.09 mmol) was added. The mixture was stirred at room temperature for 2 h. The reaction was then quenched by the addition of water (30 mL). The resulting solution was extracted with ethyl acetate (3×50 mL), the organic layers were combined and washed with brine (30 mL), dried over anhydrous Na2SO4, filtered and concentrated under vacuum. The residue was purified by silica gel column eluted with EA/hexane (v/v=40%˜80%). This resulted in 0.86 g (crude) of 4-(4-acryloylpiperazin-1-yl)-6,7-dichloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile as yellow solid. LCMS: m/z=511 [M+1]+.

Step 9. 2-bromo-3,4,5,6-tetrafluoroaniline

Into a 50-mL round-bottom flask was placed 2,3,4,5-tetrafluoroaniline (1.99 g, 12.05 mmol), Sodium acetate (1.32 g, 16.09 mmol), iron (0.10 g, 1.79 mmol) and AcOH (7 mL). The reaction mixture was heated to 45° C. This was followed by the added of bromine (3.02 g, 18.90 mmol) in AcOH (7 mL). The reaction mixture was heated to 60° C. and stirred for 1.5 h. The reaction mixture was quenched by the addition of saturated aqueous Na2S2O3(30 mL). The resulting solution was extracted with ethyl acetate (2×50 mL), the organic layers were combined and washed with saturated aqueous Na2CO3(50 mL) and brine (50 mL), dried over anhydrous Na2SO4, filtered and concentrated under vacuum. This resulted in 2.28 g (77%) of 2-bromo-3,4,5,6-tetrafluoroaniline as yellow solid. LCMS: m/z=244,246 [M+1]+.

Step 10. 2,3,4,5-tetrafluoro-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)aniline

Into a 150-mL sealed tube purged and maintained with an inert atmosphere of nitrogen, was placed 2-bromo-3,4,5,6-tetrafluoroaniline (2.28 g, 9.35 mmol), bis(pinacolato)diboron (3.96 g, 15.59 mmol), Pd(dppf)Cl2(1.21 g, 1.65 mmol), AcOK (1.84 g, 18.75 mmol) and dioxane (20 mL). The reaction mixture was stirred at 100° C. for 2 h. The reaction mixture was filtered and concentrated under vacuum. The residue was applied onto a silica gel column eluted with EA/hexane (v/v=0%-20%). This resulted in 2.28 g (81% yield) of 2,3,4,5-tetrafluoro-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)aniline as white solid. LCMS: m/z=210 [M+1]+.

Step 11. 4-(4-acryloylpiperazin-1-yl)-7-(2-amino-3,4,5,6-tetrafluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Compound 1”)

Into a 20-mL sealed tube purged and maintained with an inert atmosphere of nitrogen, was placed 4-(4-acryloylpiperazin-1-yl)-6,7-dichloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (221 mg, 0.43 mmol), 2,3,4,5-tetrafluoro-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)aniline (513 mg, 1.76 mmol), Pd(PPh3)4(151 mg, 0.13 mmol), Na2CO3(161 mg, 1.52 mmol), dioxane (5 mL) and water (0.5 mL). The reaction mixture was stirred at 80° C. for 1 h. The reaction mixture was filtered and concentrated under vacuum. The resulting crude product was further purified by C18column eluted with CH3CN/H2O (v/v=40%˜80%). This resulted in 7 mg (2% yield) of 4-(4-acryloylpiperazin-1-yl)-7-(2-amino-3,4,5,6-tetrafluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Compound 1”) as yellow solid. LCMS: m/z=640 [M+1]+.

1H NMR (400 MHz, CD3OD) δ 8.55 (s, 1H), 8.43 (d, J=5.0 Hz, 1H), 7.33-7.20 (m, 1H), 6.87 (dd, J=16.7, 10.6 Hz, 1H), 6.31 (d, J=16.9 Hz, 1H), 5.84 (d, J=10.6 Hz, 1H), 3.98 (d, J=23.5 Hz, 8H), 2.73 (d, J=28.0 Hz, 1H), 2.00 (d, J=31.9 Hz, 3H), 1.32-1.10 (m, 3H), 1.00 (dd, J=40.7, 6.8 Hz, 3H).

The mixture of 4-(4-acryloylpiperazin-1-yl)-7-(2-amino-3,4,5,6-tetrafluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile atropisomers (356 mg, several batches) was purified by Chiral-Prep-HPLC with the following conditions: Column, CHIRALPAK IG, 3 cm×25 cm, 5 um; mobile phase, CO2:EtOH=55:45; Detection wavelength, UV 220 nm. This resulted in 175 mg (49.16%) of 4-(4-acryloylpiperazin-1-yl)-7-(2-amino-3,4,5,6-tetrafluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (the first eluting isomer, “Compound 1-1”) as a yellow solid. LCMS: m/z=640 [M+1]+.

1H NMR (400 MHz, CD3OD) δ 8.45 (d, J=1.9 Hz, 1H), 8.34 (d, J=5.0 Hz, 1H), 7.17 (d, J=5.0 Hz, 1H), 6.78 (dd, J=16.8, 10.6 Hz, 1H), 6.21 (dd, J=16.8, 2.0 Hz, 1H), 5.74 (dd, J=10.6, 2.0 Hz, 1H), 4.02-3.72 (m, 8H), 2.70-2.58 (m, 1H), 1.97-1.88 (m, 3H), 1.07 (dd, J=8.2, 6.7 Hz, 3H), 0.97-0.88 (m, 3H).

And 184 mg (51.69%) of 4-(4-acryloylpiperazin-1-yl)-7-(2-amino-3,4,5,6-tetrafluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (the second eluting isomer, “Compound 1-2”) as a yellow solid. LCMS: m/z=640 [M+1]+.

1H NMR (400 MHz, CD3OD) δ 8.57 (s, 1H), 8.45 (d, J=5.0 Hz, 1H), 7.31 (d, J=4.7 Hz, 1H), 6.88 (dd, J=16.7, 10.7 Hz, 1H), 6.32 (d, J=16.8 Hz, 1H), 5.88 (d, J=10.5 Hz, 1H), 4.08-3.92 (m, 8H), 2.81-2.66 (m, 1H), 2.06-1.98 (m, 3H), 1.18 (t, J=7.4 Hz, 3H), 1.06-0.96 (in, 3H).

About 10 mg Compound 1-2 was taken into a glass vial and dissolved with 0.8 mL ethanol and 0.4 mL n-heptane. The clear solution was evaporated to dryness at room temperature through a small hole to obtain the bulk-like crystals as the sample of the testing of single crystal diffraction using the following instrument and parameters in Table 2:

TABLE 2Instrument and ParametersInstrumentSingle Crystal DiffractometerModelBruker SMART APEX IIDetector Model4K CCDSourcesEnhance Cu radiationLensTemperature293.44 KWavelength1.54 Å

The results are shown in Table 3, Table 4, Table 5, Table 6 and Table 7.

Crystallographic Data

TABLE 3Crystallographic Data and Structure Refinement for Compound 1-2Phase DataFormulaC31H26ClF4N7O2Formula Weight640.04Crystal SystemMonoclinicSpace groupC2Cell Parametersa = 20.6796 (7) Å; b = 11.2352 (4) Å;c = 14.0148 (5) Å; α = γ = 90.00°;β = 91.971 (2)Cell Ratioa/b = 1.8406; b/c = 0.8017; c/a = 0.6777Z4Cell Volume3254.3(2) Å3Calc. density1.306g/cm3Flack0.07(3)R-indices R10.0962R-indices WR20.1665Goodness-of-Fit, S1.015Rsigma0.0799Rint0.0923
Molecular Structure of Compound 1-2

TABLE 4Molecular Structure of Compound 1-2ResultsMolecular AbsolutePlanar chirality (Axis chirality)MConfiguration in singlecrystalMolecular Ball and StickFIG. 1Model in single crystalMolecular EllipsoidFIG. 2Model in single crystalStructure of Absolute  ConfigurationUnit cell StructureFIG. 3Main Hydrogen BondFIG. 4In single crystalProton Transfer in—Single crystal3D Structure-a DirectionNetworkLooking From a DirectionFIG. 53D Structure-b DirectionChained and LaminatedLooking From bFIG. 63D Structure-c DirectionChained and LaminatedLooking From c DirectionFIG. 7
Crystal Structure Solution Data

TABLE 5Atomic Coordinates (×104) and Equivalent Isotropic DisplacementParameters (Å2× 103) for Compound 1-2. U(eq) is Defined as OneThird of the Trace of the Orthogonalized UijTensor.AtomxyzU(eq)C116246.0(6)1711.4(11)4631.9(9)90.2(4)F14785.8(15)2906(3)3954(3)117.0(11)F24111.9(15)1499(4)2704(3)136.7(14)F34659(2)862(4)1040(3)148.9(16)F45807(2)1798(5)582(2)153.1(16)O16947.1(19)8554(3)4282(3)103.3(12)O26947(2)3659(5)9789(3)127.4(16)N16087.9(17)4937(3)3501(3)68.6(9)N26491.6(17)6770(4)3932(2)72.5(9)N37296(3)8781(6)6695(4)133(2)N46971.7(18)5546(4)6708(3)76.7(10)N66493(2)3262(5)1777(3)105.7(15)N75536(3)8178(6)1965(5)148(2)C15043(3)2606(5)3116(4)84.7(13)C24701(3)1929(5)2488(5)93.3(16)C34966(3)1641(6)1637(4)100.9(17)C45555(3)2081(5)1423(4)97.7(17)C55901(2)2829(4)2034(3)79.3(13)C65646(2)3082(4)2919(3)71.5(11)C76001(2)3774(4)3662(3)70.8(12)C86264(2)3232(4)4494(3)69.3(11)C96532(2)3936(4)5196(3)70.3(11)C106571(2)5161(4)5077(3)64.6(10)C116846(2)5977(5)5801(3)75.2(13)C126960(2)7121(4)5505(3)73.5(12)C136820(2)7551(5)4547(4)79.2(13)C146379(2)5597(4)4184(3)68.9(11)C157166(3)8039(6)6156(4)94.6(16)C166309(3)7206(4)2980(4)86.0(15)C176749(4)7054(5)2262(4)110(2)C186535(6)7460(8)1367(5)146(3)C195946(6)8011(11)1271(7)166(4)C205716(3)7763(6)2858(4)98.4(17)C215247(3)7954(6)3613(5)114(2)C234722(4)7012(9)3532(8)173(4)C224967(6)9207(9)3602(9)199(4)C247389(4)6458(7)2421(6)142(3)C256453(5)5039(9)7253(8)79(2)C266758(4)4128(9)7917(7)85(2)N57274(4)4605(7)8506(6)85.7(18)C277753(4)5199(8)7924(6)83.4(19)C287476(6)6148(10)7310(8)84(2)C25′6610(9)4538(16)7194(14)85(3)C26′6480(7)4955(14)8202(9)87(2)N5′7092(6)5244(12)8726(9)88.7(18)C27′7501(7)6051(13)8300(9)88(2)C28′7620(9)5650(16)7238(14)87(3)C297324(3)4417(7)9488(4)109.7(19)C307861(3)4907(7)10040(4)112(2)C318076(4)4358(8)10783(5)128(2)

TABLE 6Hydrogen Coordinates (×104) and Isotropic DisplacementParameters (Å2× 103) for Compound 1-2.AtomxyzU(eq)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

TABLE 7Anisotropic Displacement Parameters (Å2×103) for Compound 1-2. The Anisotropic Displacement Factor Exponent Takesthe Form: −2π2[h2a*2U11+ . . . + 2 h k a* b* U12]AtomU11U22U33U23U13U12C11104.2(9)71.6(7)94.8(9)11.9(6)4.1(7)−1.6(7)F191(2)142(3)120(3)−12(2)35.8(19)−15.5(19)F293(2)156(3)159(3)23(3)−15(2)−45(2)F3178(3)164(3)101(3)13(2)−45(2)−73(3)F4175(3)205(4)80(2)−41(3)9(2)−65(3)O1116(3)81(2)113(3)11(2)−1(2)−27(2)O2126(3)181(5)76(3)27(3)11(2)−32(3)N172(2)63(2)71(2)3.0(17)4.7(19)−2.8(17)N283(2)75(2)60(2)12.0(19)3.0(18)−11(2)N3137(5)136(5)125(5)−46(4)7(4)−27(4)N475(2)96(3)59(2)2.3(18)5.7(19)−17(2)N697(3)155(4)66(3)−17(3)19(2)−41(3)N7140(5)166(6)137(5)70(5)−27(4)−38(4)C180(3)90(3)85(4)2(3)12(3)−4(3)C272(3)93(4)113(4)19(3)−14(3)−25(3)C3107(4)109(4)85(4)15(3)−33(3)−27(4)C4109(4)128(5)55(3)−7(3)−6(3)−19(3)C589(3)87(3)62(3)5(2)0(3)−11(3)C675(3)73(3)66(3)−1(2)5(2)−12(2)C768(3)76(3)70(3)2(2)21(2)0(2)C877(3)60(3)71(3)15(2)7(2)−1(2)C968(3)80(3)62(3)2(2)9(2)−2(2)C1068(3)68(3)58(2)9.1(18)5(2)−3(2)C1164(3)98(4)64(3)−4(2)11(2)−12(2)C1268(3)84(3)70(3)−9(2)9(2)−18(2)C1373(3)83(3)82(3)3(3)6(2)−15(3)C1469(3)65(3)73(3)2(2)20(2)−10(2)C1595(4)102(4)88(4)−12(3)15(3)−10(3)C16111(4)78(3)69(3)15(2)1(3)−22(3)C17155(6)101(4)76(4)20(3)29(4)−26(4)C18219(10)138(6)82(5)28(4)21(6)−29(6)C19186(9)200(10)111(7)70(6)−19(7)−54(8)C2095(4)110(4)90(4)35(3)−6(3)−22(3)C2185(4)111(5)145(6)29(4)2(4)1(3)C23101(5)180(9)239(10)−11(7)35(6)−29(5)C22213(10)132(7)252(12)30(7)−6(9)37(7)C24162(6)114(6)156(7)6(4)67(5)10(5)C2572(5)94(5)71(5)14(4)9(4)−14(4)C2679(5)95(5)81(5)13(4)7(4)−16(4)N592(4)91(5)74(4)12(3)8(3)−7(4)C2788(5)97(5)65(4)0(4)0(4)−16(4)C2887(5)97(6)67(5)−2(4)0(4)−15(4)C25′90(6)93(6)72(6)7(5)12(5)−12(5)C26′92(5)95(5)74(5)7(4)11(4)−8(4)N5′97(4)95(5)75(4)8(4)7(3)−9(4)C27′97(5)93(5)75(5)8(4)5(4)−12(4)C28′95(6)91(6)75(6)7(5)3(5)−16(5)C29107(5)150(6)72(4)10(3)11(3)−23(4)C30138(6)131(5)68(4)3(3)−1(4)−18(4)C31129(6)164(6)90(5)−6(4)9(4)−4(5)

The following conclusions were obtained by crystal structure analysis:

The crystal system of Compound 1-2 is monoclinic and has two symmetries and one centering vector, space group is C2.

The single crystal of Compound 1-2 have four molecules in a unit cell.

Compound 1-2 has one planar chirality (axis chirality). Absolute configuration is M.

Unit cell has two kinds of hydrogen bonds, they are respectively O2-H6A-N6, N3-H6B—N6. The three-dimensional structure of the chain, laminate and network is formed through hydrogen bond and van der Waals force in the crystal.

Example 2

4-(4-acryloylpiperazin-1-yl)-7-(3-amino-2,6-dichloro-4,5-difluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Compound 2”)

Step 1. 2-(3,4-difluoro-5-nitrophenyl)-4,4,5,5-tetramethyl-1,3,2-dioxaborolane

Into a 40-mL sealed tube purged and maintained with an inert atmosphere of nitrogen, was placed 5-bromo-1,2-difluoro-3-nitrobenzene (207 mg, 0.38 mmol), bis(pinacolato)diboron (2.16 g, 8.51 mmol), Pd(dppf)Cl2(0.322 g, 0.44 mmol), KOAc (1.199 g, 12.22 mmol) and dioxane (10 mL). The reaction mixture was stirred at 100° C. for 2 h. The reaction mixture was filtered and concentrated under vacuum. The residue was applied onto a silica gel column eluted with EA/hexane (v/v=0%-5%). This resulted in 1.528 g (crude) of 2-(3,4-difluoro-5-nitrophenyl)-4,4,5,5-tetramethyl-1,3,2-dioxaborolane as off-white solid.

Step 2. 2,3-difluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)aniline

Into a 40-mL round-bottom flask was placed 2-(3,4-difluoro-5-nitrophenyl)-4,4,5,5-tetramethyl-1,3,2-dioxaborolane (1.489 g, 5.22 mmol), iron (1.546 g, 27.68 mmol), NH4Cl (2.654 g, 49.62 mmol), EtOH (15 mL) and H2O (5 mL). The reaction mixture was stirred at 80° C. for 1 h. The reaction mixture was filtered and concentrated under vacuum. The residue was washed with DCM (20 mL), filtered and concentrated under vacuum. This resulted in 0.957 g of 2,3-difluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)aniline as off-white solid. LCMS: m/z=256 [M+1]+.

Step 3. 4-(4-acryloylpiperazin-1-yl)-7-(3-amino-4,5-difluorophenyl)-6-chloro-1-(2-isopropyl-4-methyl pyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile

Into a 20-mL sealed tube purged and maintained with an inert atmosphere of nitrogen, was placed 4-(4-acryloylpiperazin-1-yl)-6,7-dichloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-na phthyridine-3-carbonitrile (0.21 g, 0.41 mmol), 2,3-difluoro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)aniline (0.279 g, 1.09 mmol), Pd(PPh3)4(57 mg, 0.05 mmol), Na2CO3(206 mg, 1.94 mmol), dioxane (5 mL) and water (1 mL). The reaction mixture was stirred at 80° C. for 1 h. The reaction mixture was filtered and concentrated under vacuum. The residue was applied onto a silica gel column eluted with EA/hexane (v/v=50%-100%). This resulted in 125 mg of 4-(4-acryloylpiperazin-1-yl)-7-(3-amino-4,5-difluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile as yellow solid. LCMS: m/z=604 [M+1]+.

Step 4. 4-(4-acryloylpiperazin-1-yl)-7-(3-amino-2,6-dichloro-4,5-difluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Compound 2”)

Into a 50-mL round-bottom flask purged and maintained with an inert atmosphere of nitrogen, was placed 4-(4-acryloylpiperazin-1-yl)-7-(3-amino-4,5-difluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (103 mg, 170.52 μmol), NCS (51 mg, 381.93 μmol) and DMA (1.5 mL). The reaction mixture was stirred for 15 h at room temperature. The reaction was then quenched by the addition of H2O (20 mL). The resulting solution was extracted with ethyl acetate (3×20 mL), the organic layers were combined and concentrated under vacuum. The residues was purified by prep-HPLC eluted with CH3CN/H2O (v/v=7/1). This resulted in 30 mg (26%) of 4-(4-acryloylpiperazin-1-yl)-7-(3-amino-2,6-dichloro-4,5-difluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Compound 2”). LCMS: m/z=672 [M+1]+.

1H NMR (400 MHz, CD3OD) δ 8.56 (s, 1H), 8.40 (d, J=5.0 Hz, 1H), 7.29-7.15 (m, 1H), 6.87 (dd, J=16.8, 10.6 Hz, 1H), 6.30 (d, J=16.8 Hz, 1H), 5.83 (d, J=10.7 Hz, 1H), 4.30-3.77 (m, 8H), 2.85-2.66 (m, 1H), 1.99 (s, 3H), 1.18 (d, J=6.7 Hz, 3H), 1.01 (d, J=6.8 Hz, 3H).

Example 3

4-(4-acryloylpiperazin-1-yl)-7-(2-amino-3-chloro-4,5,6-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Compound 3”)

Step 1. 2-bromo-3,4,5-trifluoroaniline

Into a 100-mL round-bottom flask was placed 2-bromo-3,4,5-trifluoro-1-nitrobenzene (2.64 g, 10.31 mmol), iron (5.71 g, 102.25 mmol), ammonium chloride (5.67 g, 106.00 mmol), ethanol (25 mL) and water (25 mL). The reaction mixture was heated to 55° C. and stirred for 1.5 h. The reaction mixture was filtered and the filtrate was concentrated under vacuum. The residues was dissolved in ethanol (30 mL) and applied onto a C18column eluted with CH3CN/H2O (v:v=9:1). This resulted in 1.33 g (57%) of 2-bromo-3,4,5-trifluoroaniline. LCMS: m/z=226, 228 [M+1]+.

Step 2. 3,4,5-trifluoro-2-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)aniline

Into a 40-mL sealed tube purged and maintained with an inert atmosphere of nitrogen, was placed 2-bromo-3,4,5-trifluoroaniline (2.99 g, 13.23 mmol), bis(pinacolato)diboron (10.20 g, 40.17 mmol), Pd(dppf)Cl2(0.98 g, 1.34 mmol), KOAc (4.18 g, 42.59 mmol) and dioxane (40 mL). The reaction mixture was stirred at 100° C. for 5 h. The reaction mixture was concentrated under vacuum. The residue was applied onto a silica gel column eluted with EA/hexane (v/v=0%-10%). This resulted in 5.34 g (crude) of 3,4,5-trifluoro-2-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)aniline.

Step 3. 4-(4-acryloylpiperazin-1-yl)-7-(6-amino-2,3,4-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile

Into a 20-mL sealed tube purged and maintained with an inert atmosphere of nitrogen, was placed 4-(4-acryloylpiperazin-1-yl)-6,7-dichloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-na phthyridine-3-carbonitrile (0.547 g, 1.08 mmol), 3,4,5-trifluoro-2-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)aniline (0.279 g, 1.09 mmol), Pd(PPh3)4(1.376 g, 5.04 mmol), Na2CO3(0.347 g, 3.27 mmol), dioxane (5 mL) and water (1 mL). The reaction mixture was stirred at 80° C. for 4 h. The reaction mixture was filtered and concentrated under vacuum. The residue was applied onto a silica gel column eluted with EA/hexane (v/v=50%-100%). This resulted in 115 mg of 4-(4-acryloylpiperazin-1-yl)-7-(6-amino-2,3,4-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile as yellow solid. LCMS: m/z=622 [M+1]+.

Step 4. 4-(4-acryloylpiperazin-1-yl)-7-(2-amino-3-chloro-4,5,6-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Compound 3”)

Into a 50-mL round-bottom flask purged and maintained with an inert atmosphere of nitrogen, was placed 4-(4-acryloylpiperazin-1-yl)-7-(6-amino-2,3,4-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (100 mg, 160.76 μmol), NCS (0.035 g, 262.11 μmol) and AcOH (1 mL). The reaction mixture was stirred for 7 h at 35° C. and 15 h at room temperature.

The reaction was then quenched by the addition of saturated aqueous NaHCO3(30 mL). The resulting solution was extracted with ethyl acetate (2×10 mL), the organic layers were combined and concentrated under vacuum. The residues was purified by prep-HPLC eluted with CH3CN/H2O (v/v=6/1). This resulted in 6 mg (5%) of 4-(4-acryloylpiperazin-1-yl)-7-(2-amino-3-chloro-4,5,6-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Com pound 3”) as yellow solid. LCMS: m/z=656 [M+1]+.

1H NMR (400 MHz, CD3OD) δ 8.66-8.51 (m, 1H), 8.43 (d, J=5.1 Hz, 1H), 7.26 (d, J=5.1 Hz, 1H), 6.88 (dd, J=16.8, 10.7 Hz, 1H), 6.31 (d, J=16.7 Hz, 1H), 5.84 (d, J=10.7 Hz, 1H), 4.06-3.92 (m, 8H), 2.84-2.62 (m, 1H), 2.06-1.92 (m, 3H), 1.17 (dd, J=9.6, 7.0 Hz, 3H), 1.06-0.94 (m, 3H).

Example 4

4-(4-acryloylpiperazin-1-yl)-7-(2-amino-3,5-dichloro-4,6-difluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Compound 4”)

Step 1. 3,5-difluoro-2-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)aniline

Into a 100-mL sealed tube purged and maintained with an inert atmosphere of nitrogen, was placed 2-bromo-3,5-difluoroaniline (3.01 g, 14.47 mmol), bis(pinacolato)diboron (6.46 g, 25.43 mmol), Pd(dppf)Cl2(1.03 g, 1.41 mmol), KOAc (4.20 g, 42.83 mmol), dioxane (20 mL). The reaction mixture was stirred at 80° C. for 12 h. The reaction mixture was filtered and concentrated under vacuum. The residue was purified by silica gel column eluted with EA/hexane (v/v=1/19). This resulted in 6.01 g (crude) of 3,5-difluoro-2-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)aniline as yellow oil. LCMS: m/z=256 [M+1]+.

Step 2. tert-butyl 4-(7-(2-amino-4,6-difluorophenyl)-6-chloro-3-cyano-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridin-4-yl)piperazine-1-carboxylate

Into a 100-mL sealed tube purged and maintained with an inert atmosphere of nitrogen, was placed 3,5-difluoro-2-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)aniline (5.86 g, 23.00 mmol), tert-butyl 4-(6,7-dichloro-3-cyano-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridin-4-yl)p iperazine-1-carboxylate (1.29 g, 2.31 mmol), Pd(PPh3)4(0.73 g, 0.63 mmol), Na2CO3(0.84 g, 7.89 mmol), dioxane (15 mL) and water (2 mL). The reaction mixture was stirred at 80° C. for 2 h. The reaction mixture was filtered and concentrated under vacuum. The residue was purified by silica gel column eluted with EA/hexane (v/v=1/1). This resulted in 1.42 g (crude) of tert-butyl 4-(7-(2-amino-4,6-difluorophenyl)-6-chloro-3-cyano-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridin-4-yl)piperazine-1-carboxylate as yellow solid. LCMS: m/z=650 [M+1]+.

Step 3. tert-butyl 4-(7-(2-amino-3,5-dichloro-4,6-difluorophenyl)-6-chloro-3-cyano-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridin-4-yl)piperazine-1-carboxylate

Into a 50-mL round-bottom flask purged and maintained with an inert atmosphere of nitrogen, was placed tert-butyl 4-(7-(2-amino-4,6-difluorophenyl)-6-chloro-3-cyano-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridin-4-yl)piperazine-1-carboxylate (1.21 g, 1.87 mmol), NCS (0.49 g, 3.73 mmol) and AcOH (30 mL). The reaction mixture was stirred for 48 h at room temperature. The reaction was then quenched by the addition of water (100 mL). The resulting solution was extracted with ethyl acetate (2×100 mL), the organic layers were combined and washed with brine (10 mL), dried over anhydrous Na2SO4, filtered and concentrated under vacuum. The residue was purified by silica gel column eluted with EA/hexane (v/v=1/1). This resulted in 0.53 g (crude) of tert-butyl 4-(7-(2-amino-3,5-dichloro-4,6-difluorophenyl)-6-chloro-3-cyano-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridin-4-yl)piperazine-1-carboxylate as yellow solid. LCMS: m/z=718[M+1]+.

Step 4. 4-(4-acryloylpiperazin-1-yl)-7-(2-amino-3,5-dichloro-4,6-difluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Compound 4”)

Into a 20-mL round-bottom flask purged and maintained with an inert atmosphere of nitrogen, was placed tert-butyl 4-(7-(2-amino-3,5-dichloro-4,6-difluorophenyl)-6-chloro-3-cyano-1-(2-isopropyl-4-methyl pyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridin-4-yl)piperazine-1-carboxylate (0.53 g, 0.73 mmol), TFA (5 ml) and DCM (20 mL). The reaction mixture was stirred at room temperature for 2 h. The reaction mixture was concentrated under vacuum. The residue was dissolved by DCM (10 mL) in 25-mL round-bottom flask. DIEA (1.22 g, 9.48 mmol) was added. The reaction mixture was cooled to 0° C. and acryloyl chloride (0.08 g, 0.88 mmol) was added. The mixture was stirred at room temperature for 0.5 h. The reaction mixture was filtered and concentrated under vacuum. The residue was purified by Prep-HPLC (CH3CN/H2O (v/v=7/3)). This resulted in 239 mg (50% in two steps) of 4-(4-acryloylpiperazin-1-yl)-7-(2-amino-3,5-dichloro-4,6-difluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Compound 4”) as yellow solid. LCMS: m/z=672 [M+1]+.

1H NMR (400 MHz, CD3OD) δ 8.55 (s, 1H), 8.43 (s, 1H), 7.26 (s, 1H), 6.87 (dd, J=16.1, 11.1 Hz, 1H), 6.31 (d, J=16.3 Hz, 1H), 5.84 (d, J=10.1 Hz, 1H), 4.25-3.75 (m, 8H), 2.85-2.60 (m, 1H), 2.14-1.88 (m, 3H), 1.25-0.87 (m, 6H).

Example 5

4-((3S,5R)-4-acryloyl-3,5-dimethylpiperazin-1-yl)-7-(2-amino-3,5-dichloro-4,6-difluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Compound 5”)

Step 1. 4-((3R,5S)-4-acryloyl-3,5-dimethylpiperazin-1-yl)-6,7-dichloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (Intermediate D)

Into a 500-mL round-bottom flask purged and maintained with an inert atmosphere of nitrogen, was placed 6,7-dichloro-4-hydroxy-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (6.21 g, 15.95 mmol), POCl3(6.88 g, 48.87 mmol), DIEA (6.80 g, 52.61 mmol) and acetonitrile (100 mL). The mixture was stirred for 2 h at 80° C. The reaction was cooled to room temperature and concentrated under vacuum. This resulted in 4,6,7-trichloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile which was used directly in the next step.

Into a 500-mL round-bottom flask purged and maintained with an inert atmosphere of nitrogen, was placed 4,6,7-trichloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (crude), DIEA (6.80 g, 52.61 mmol) and acetonitrile (100 mL), (2S,6R)-2,6-dimethylpiperazine (2.17 g, 19.00 mmol) was added. The mixture was stirred for 1 h at room temperature. The resulting solution was concentrated under vacuum and applied onto a silica gel column eluted with EA/hexane (v/v=2/1). This resulted in 4.30 g (50% yield) of 4-((3R,5S)-4-acryloyl-3,5-dimethylpiperazin-1-yl)-6,7-dichloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-na phthyridine-3-carbonitrile as yellow solid. LCMS: m/z=539 [M+1]+.

Step 2. 3,5-difluoro-2-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)aniline

Into a 40-mL sealed tube purged and maintained with an inert atmosphere of nitrogen, was placed 2-bromo-3,5-difluoroaniline (2.06 g, 9.91 mmol), bis(pinacolato)diboron (4.95 g, 19.49 mmol), Pd(dppf)Cl2(809 mg, 1.11 mmol), KOAc (2.23 g, 22.69 mmol), dioxane (20 mL). The reaction mixture was stirred at 100° C. for 19 h. The reaction mixture was concentrated under vacuum. The residue was applied onto a C18column eluted with CH3CN/H2O (v/v=5%-100%). This resulted in 1.19 g of 3,5-difluoro-2-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)aniline as off-white solid. LCMS: m/z=256 [M+1]+.

Step 3. 4-((3S,5R)-4-acryloyl-3,5-dimethylpiperazin-1-yl)-7-(2-amino-4,6-difluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydroquinoline-3-carbonitrile

Into a 20-mL sealed tube purged and maintained with an inert atmosphere of nitrogen, was placed 4-((3S,5R)-4-acryloyl-3,5-dimethylpiperazin-1-yl)-6,7-dichloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydroquinoline-3-carbonitrile (0.196 g, 0.36 mmol), 3,5-difluoro-2-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)aniline (0.27 g, 1.06 mmol), Pd(PPh3)4(78 mg, 0.07 mmol), Na2CO3(207 mg, 1.95 mmol), dioxane (5 mL) and water (1 mL). The reaction mixture was stirred at 80° C. for 1 h. The reaction mixture was filtered and concentrated under vacuum. The residues was applied onto a silica gel column eluted with EA/hexane (v/v=50%-100%). This resulted in 223 mg of 4-((3S,5R)-4-acryloyl-3,5-dimethylpiperazin-1-yl)-7-(2-amino-4,6-difluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydroquinoline-3-carbonitrile as yellow solid. LCMS: m/z=632 [M+1]+.

Step 4. 4-((3S,5R)-4-acryloyl-3,5-dimethylpiperazin-1-yl)-7-(2-amino-3,5-dichloro-4,6-difluorophenyl)-6-chl oro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydroquinoline-3-carbonitrile (“Compound 5”)

Into a 20-mL round-bottom flask was placed 4-((3S,5R)-4-acryloyl-3,5-dimethylpiperazin-1-yl)-7-(2-amino-4,6-difluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydroquinoline-3-carbonitrile (223 mg, 0.35 mmol), NCS (98 mg, 0.73 mmol) and HOAc (3 mL). The reaction mixture was stirred at room temperature for 1 day. The reaction was heated to 45° C. and stirred at this temperature for 2 hours. The reaction mixture was concentrated under vacuum. The residues was purified by Prep-HPLC CH3CN/H2O (0.05% NH4HCO3) (v/v=2/1). This resulted in 55 mg (22%) of 4-((3S,5R)-4-acryloyl-3,5-dimethylpiperazin-1-yl)-7-(2-amino-3,5-dichloro-4,6-difluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydroquinoline-3-carbonitrile (“Compound 5”) as yellow solid. LCMS: m/z=700 [M+1]+.

1H NMR (400 MHz, CD3OD) δ 8.83 (s, 1H), 8.43 (d, J=5.0 Hz, 1H), 7.27 (d, J=5.0 Hz, 1H), 6.95-6.82 (m, 1H), 6.33 (d, J=16.5 Hz, 1H), 5.84 (d, J=10.8 Hz, 1H), 4.77 (s, 2H), 4.13-3.92 (m, 2H), 3.84 (d, J=9.4 Hz, 2H), 2.80-2.60 (m, 1H), 2.08-1.93 (m, 3H), 1.72-1.59 (m, 6H), 1.25-1.10 (m, 3H), 1.10-0.89 (m, 3H).

Example 6

4-(4-acryloylpiperazin-1-yl)-7-(3-amino-2,4,5,6-tetrafluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Compound 6”)

Step 1. 4-(4-acryloylpiperazin-1-yl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-7-(tributylstannyl)-1,2-dihydro-1,8-naphthyridine-3-carbonitrile

Into a 30-mL sealed tube purged and maintained with an inert atmosphere of nitrogen, was placed 4-(4-acryloylpiperazin-1-yl)-6,7-dichloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-na phthyridine-3-carbonitrile (1.039 g, 2.04 mmol), 1,1,1,2,2,2-Hexabutyl-distannane (4.124 g, 7.11 mmol), Pd(PPh3)4(0.566 g, 0.49 mmol) and dioxane (10 mL). The reaction mixture was stirred at 100° C. for 1 d. The reaction mixture was filtered and concentrated under vacuum. The residues was purified by silica gel column eluted with EA/hexane (v/v=2/1). This resulted in 273 mg (17%) of 4-(4-acryloylpiperazin-1-yl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-7-(tributylstannyl)-1,2-dihydro-1,8-naphthyridine-3-carbonitrile as yellow solid. LCMS: m/z=767 [M+1]+.

Step 2. 4-(4-acryloylpiperazin-1-yl)-7-(3-amino-2,4,5,6-tetrafluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Compound 6”)

Into a 25-mL round-bottom flask purged and maintained with an inert atmosphere of nitrogen, was placed 4-(4-acryloylpiperazin-1-yl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-7-(tributylstannyl)-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (0.272 g, 0.36 mmol), 2,3,4,6-tetrafluoro-5-iodoaniline (0.630 g, 2.17 mmol), Pd(PPh3)4(0.187 g, 0.16 mmol), cuprous iodide (0.294 g, 1.54 mmol), dioxane (10 mL). The reaction mixture was stirred at 100° C. for 1 d. The reaction mixture was filtered and concentrated under vacuum. The residues was purified by prep-HPLC eluted with ACN/H2O (v/v=1/2). This resulted in 6 mg (3%) of 4-(4-acryloylpiperazin-1-yl)-7-(3-amino-2,4,5,6-tetrafluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Compound 6”) as yellow solid. LCMS: m/z=640 [M+1]+.

1H NMR (400 MHz, CD3OD) δ 8.56 (s, 1H), 8.42 (d, J=4.9 Hz, 1H), 7.31-7.20 (m, 1H), 6.87 (dd, J=16.7, 10.6 Hz, 1H), 6.30 (dd, J=16.8, 1.7 Hz, 1H), 5.83 (dd, J=10.6, 1.8 Hz, 1H), 4.08-3.87 (m, 8H), 2.79-2.62 (m, 1H), 2.00 (s, 3H), 1.17 (d, J=6.8 Hz, 3H), 0.98 (d, J=6.7 Hz, 3H).

Example 7

4-((3S,5R)-4-acryloyl-3,5-dimethylpiperazin-1-yl)-7-(3-amino-2-chloro-4,5,6-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Compound 7”)

Step 1. 4-((3S,5R)-4-acryloyl-3,5-dimethylpiperazin-1-yl)-7-(5-amino-2,3,4-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile

Into a 30-mL sealed tube purged and maintained with an inert atmosphere of nitrogen, was placed 4-((3S,5R)-4-acryloyl-3,5-dimethylpiperazin-1-yl)-6,7-dichloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (0.822 g, 1.52 mmol), (5-amino-2,3,4-trifluorophenyl) boronic acid (0.608 g, 2.23 mmol), Pd(PPh3)4(0.650 g, 0.56 mmol), Na2CO3(0.575 g, 5.43 mmol), dioxane (10 mL) and water (2 mL). The reaction mixture was stirred at 80° C. for 1 h. The reaction mixture was filtered and concentrated under vacuum. The residue was purified by silica gel column eluted with EA/hexane (v/v=2/1). This resulted in 1.079 g (91%) of 4-((3S,5R)-4-acryloyl-3,5-dimethylpiperazin-1-yl)-7-(5-amino-2,3,4-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile as yellow solid. LCMS: m/z=650 [M+1]+.

Step 2. 4-((3S,5R)-4-acryloyl-3,5-dimethylpiperazin-1-yl)-7-(3-amino-2-chloro-4,5,6-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Compound 7”)

Into a 50-mL round-bottom flask purged and maintained with an inert atmosphere of nitrogen, was placed 4-((3S,5R)-4-acryloyl-3,5-dimethylpiperazin-1-yl)-7-(5-amino-2,3,4-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (0.622 g, 0.96 mmol), NCS (0.703 g, 5.27 mmol), and AcOH (5 mL). The mixture was stirred at r.t. for 2 d. The reaction was then quenched by the addition of water (10 mL). The resulting solution was extracted with ethyl acetate (3×10 mL), the organic layers were combined and washed with brine (1×50 mL), dried over anhydrous Na2SO4, filtered and concentrated under vacuum. The residues was purified by Prep-HPLC (CH3CN/H2O (v/v=6/4)). This resulted in 35 mg (5%) of 4-((3S,5R)-4-acryloyl-3,5-dimethylpiperazin-1-yl)-7-(3-amino-2-chloro-4,5,6-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Compound 7”) as yellow solid. LCMS: m/z=684 [M+1]+.

1H NMR (400 MHz, CD3OD) δ 8.83 (s, 1H), 8.42 (d, J=5.0 Hz, 1H), 7.22-7.10 (m, 1H), 6.88 (dd, J=16.7, 10.7 Hz, 1H), 6.32 (d, J=16.5 Hz, 1H), 5.74 (dd, J=10.6, 2.0 Hz, 1H), 4.76 (s, 2H), 4.06-3.73 (m, 4H), 2.82-2.69 (m, 1H), 2.10-1.94 (m, 3H), 1.63-1.35 (m, 6H), 1.17 (d, J=6.5 Hz, 3H), 1.06-0.89 (m, 3H).

Example 8

4-((3S,5R)-4-acryloyl-3,5-dimethylpiperazin-1-yl)-7-(3-amino-2,4,5,6-tetrafluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Compound 8”)

Step 1. 4-((3S,5R)-4-acryloyl-3,5-dimethylpiperazin-1-yl)-6,7-dichloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile

Into a 20-mL round-bottom flask purged and maintained with an inert atmosphere of nitrogen, was placed 6,7-dichloro-4-((3S,5R)-3,5-dimethylpiperazin-1-yl)-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (6.503 g, 13.39 mmol), DIEA (5.21 g, 40.31 mmol), DCM (6 mL) and acryloyl chloride (1.18 g, 13.04 mmol). The reaction mixture was stirred at room temperature for 0.5 h. The reaction mixture was filtered and concentrated under vacuum. The residue was purified by silica gel column eluted with EA/hexane (v/v=1/1). This resulted in 1.80 g (crude) of 4-((3S,5R)-4-acryloyl-3,5-dimethylpiperazin-1-yl)-6,7-dichloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile as yellow solid. LCMS: m/z=539 [M+1]+.

Step 2. 4-((3S,5R)-4-acryloyl-3,5-dimethylpiperazin-1-yl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-7-(tributylstannyl)-1,2-dihydro-1,8-naphthyridine-3-carbonitrile

Into a 20-mL sealed tube purged and maintained with an inert atmosphere of nitrogen, was placed 4-((3S,5R)-4-acryloyl-3,5-dimethylpiperazin-1-yl)-6,7-dichloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (1.77 g, 3.28 mmol), hexabutyldistannane (23.85 g, 6.64 mmol), Pd(PPh3)4(0.26 g, 0.23 mmol) and dioxane (20 mL). The reaction mixture was stirred at 110° C. for 18 h. The reaction mixture was filtered and concentrated under vacuum. The residue was purified by silica gel column eluted with EA/hexane (v/v=2/1). This resulted in 0.52 g (crude) of 4-((3S,5R)-4-acryloyl-3,5-dimethylpiperazin-1-yl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-7-(tributylstannyl)-1,2-dihydro-1,8-naphthyridine-3-carbonitrile as yellow solid. LCMS: m/z=795 [M+1]+.

Step 3. 4-((3S,5R)-4-acryloyl-3,5-dimethylpiperazin-1-yl)-7-(3-amino-2,4,5,6-tetrafluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Compound 8”)

Into a 20-mL round-bottom flask purged and maintained with an inert atmosphere of nitrogen, was placed 4-((3S,5R)-4-acryloyl-3,5-dimethylpiperazin-1-yl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-7-(tributylstannyl)-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (0.40 g, 0.51 mmol), 2,3,4,6-tetrafluoro-5-iodoaniline (0.27 g, 0.93 mmol), Pd(PPh3)4(0.27 g, 0.23 mmol), CuI (0.40 g, 2.11 mmol), DMA (15 mL). The reaction mixture was stirred at 90° C. for 18 h. The reaction mixture was filtered and concentrated under vacuum. The residue was purified by Prep-HPLC (CH3CN/H2O (v/v=7/3)). This resulted in 8 mg (2.3% in two steps) of 4-((3S,5R)-4-acryloyl-3,5-dimethylpiperazin-1-yl)-7-(3-amino-2,4,5,6-tetrafluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Compound 8”) as yellow solid. LCMS: m/z=668 [M+1]+.

1H NMR (400 MHz, CD3OD) δ 8.73 (s, 1H), 8.34 (d, J=5.0 Hz, 1H), 7.23-7.15 (m, 1H), 6.79 (dd, J=16.7, 10.7 Hz, 1H), 6.23 (dd, J=16.7, 2.0 Hz, 1H), 5.74 (dd, J=10.6, 2.0 Hz, 1H), 4.73-4.58 (m, 2H), 3.95-3.70 (m, 4H), 2.66-2.57 (m, 1H), 1.93 (d, J=8.4 Hz, 3H), 1.55-1.57 (m, 6H), 1.07 (d, J=6.8 Hz, 3H), 0.89 (d, J=5.7 Hz, 3H).

Example 9

(P)-4-((R)-4-acryloyl-3-methylpiperazin-1-yl)-7-(2-amino-3,4,5,6-tetrafluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile; or (M)-4-((R)-4-acryloyl-3-methylpiperazin-1-yl)-7-(2-amino-3,4,5,6-tetrafluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile; (“Compound 9”)

Step 1. 6,7-dichloro-4-hydroxy-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile

Into a 250-mL round-bottom flask purged and maintained with an inert atmosphere of nitrogen, was placed 2-cyano-N-(2-isopropyl-4-methylpyridin-3-yl)-3-oxo-3-(2,5,6-trichloropyridin-3-yl)propanamide (5.89 g, 13.83 mmol) and THF (70 mL), the mixture was stirred at room temperature. This was followed by the addition of NaH (2.73 g, 68.25 mmol) in batch-wise. The mixture was stirred at 50° C. for 2 h. The reaction mixture was concentrated under vacuum. The residue was dissolved in 100 mL water and adjusted pH to 7 with AcOH. The resulting solid was filtered and dried under vacuum to provide 5.85 g (108% in two steps) of 6,7-dichloro-4-hydroxy-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile as yellow solid. LCMS: m/z=389 [M+1]+.

1H NMR (400 MHz, DMSO-d6) δ 8.77 (d, J=5.7 Hz, 1H), 8.38 (s, 1H), 7.89 (d, J=5.4 Hz, 1H), 3.01-2.88 (m, 1H), 2.19 (s, 3H), 1.21 (d, J=6.9 Hz, 3H), 1.14 (d, J=6.9 Hz, 3H).

The mixture of 6,7-dichloro-4-hydroxy-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile atropisomers (10.59 g, “Intermediate B”, several batches) was purified by Chiral-Prep-HPLC with the following conditions: Column, CHIRALPAK IC, 3.0×100 mm, 3 μm; mobile phase, IPA/ACN=1/1(V/V); Detection wavelength, UV 210 nm. This resulted in 4.99 g (47%) of 6,7-dichloro-4-hydroxy-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-car bonitrile (the first eluting isomer, “Intermediate B-1”, M or P atropisomer) as a brown solid;

1H NMR (400 MHz, CD3OD) δ 8.48 (s, 1H), 8.44 (d, J=5.0 Hz, 1H), 7.33-7.22 (m, 1H), 2.76-2.61 (m, 1H), 2.03 (s, 3H), 1.19 (d, J=6.8 Hz, 3H), 1.06 (d, J=6.8 Hz, 3H).

And 4.60 g (43%) of 6,7-dichloro-4-hydroxy-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (the second eluting isomer, “Intermediate B-2”, P or M atropisomer) as a brown solid;

1H NMR (400 MHz, CD3OD) δ 8.49 (s, 1H), 8.44 (d, J=5.0 Hz, 1H), 7.28 (d, J=5.0 Hz, 1H), 2.76-2.63 (m, 1H), 2.03 (s, 3H), 1.19 (d, J=6.8 Hz, 3H), 1.079-1.00 (m, 3H).

Step 2. tert-butyl (R)-4-(6,7-dichloro-3-cyano-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridin-4-yl)-2-methylpiperazine-1-carboxylate (single isomer)

Into a 50-mL round-bottom flask purged and maintained with an inert atmosphere of nitrogen, was placed 6,7-dichloro-4-hydroxy-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-car bonitrile (the first eluting isomer in step 1) (1.05 g, 2.71 mmol), POCl3(2.04 g, 13.27 mmol), DIEA (3.09 g, 23.93 mmol) and acetonitrile (20 mL). The mixture was stirred at 80° C. for 2 h. The reaction was cooled to room temperature and concentrated under vacuum. This resulted in 4,6,7-trichloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile which was used directly in the next step.

Into a 100-mL round-bottom flask purged and maintained with an inert atmosphere of nitrogen, was placed 4,6,7-trichloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (crude) and acetonitrile (10 mL), DIEA (3.09 g, 23.93 mmol) and tert-butyl (R)-2-methylpiperazine-1-carboxylate (507 mg, 2.53 mmol) were added. The reaction mixture was stirred for 2 h at room temperature. The reaction was then quenched by the addition of water (50 mL). The resulting solution was extracted with ethyl acetate (3×50 mL), the organic layers were combined and washed with brine (50 mL), dried over anhydrous Na2SO4, filtered and concentrated under vacuum. The residue was purified by silica gel column eluted with EA/hexane (v/v=5/4). This resulted in 0.95 g (65% in two steps) of tert-butyl (R)-4-(6,7-dichloro-3-cyano-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridin-4-yl)-2-methylpiperazine-1-carboxylate (single isomer, P or M) as red solid. LCMS: m/z=571 [M+1]+.

Step 3. (R)-4-(4-acryloyl-3-methylpiperazin-1-yl)-6,7-dichloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile

Into a 50-mL round-bottom flask purged and maintained with an inert atmosphere of nitrogen, was placed tert-butyl (R)-4-(6,7-dichloro-3-cyano-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridin-4-yl)-2-methylpiperazine-1-carboxylate (P or M isomer, 1.01 g, 1.767 mmol), DCM (8 mL) and TFA (4.605 g, 40.386 mmol). The reaction mixture was stirred at room temperature for 1 h. The reaction mixture was concentrated under vacuum. The residue was dissolved by DCM (10 mL) into a 50 mL round-bottom flask, triethylamine (1.30 g, 12.85 mmol) was added. The reaction mixture was cooled to 0° C. and acryloyl chloride (0.23 g, 2.541 mmol) was added. The mixture stirred at room temperature for 0.5 h. The reaction was then quenched by the addition of water (20 mL). The resulting solution was extracted with ethyl acetate (3×20 mL), the organic layers were combined and washed with brine (20 mL), dried over anhydrous Na2SO4, filtered and concentrated under vacuum. The residues was purified by Prep-HPLC CH3CN/H2O (v/v=3/2). This resulted in 1.46 g (crude) of (R)-4-(4-acryloyl-3-methylpiperazin-1-yl)-6,7-dichloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (P or M isomer) as yellow solid. LCMS: m/z=525 [M+1]+.

Step 4. 4-((R)-4-acryloyl-3-methylpiperazin-1-yl)-7-(2-amino-3,4,5,6-tetrafluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (P or M isomer, “Compound 9”)

Into a 100 mL round bottom flask purged and maintained with an inert atmosphere of nitrogen was placed (R)-4-(4-acryloyl-3-methylpiperazin-1-yl)-6,7-dichloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (P or M isomer, 1.64 g, 3.11 mmol), (2-amino-3,4,5,6-tetrafluorophenyl)boronic acid (1.37 g, 6.57 mmol), Pd(PPh3)4(0.37 g, 315.86 mol), Na2CO3(1.11 g, 10.49 mmol), 1,4-Dioxane (15 mL) and Water (2 mL). The resulting mixture was stirred at 80° C. for 30 min. Three batches of (2-amino-3,4,5,6-tetrafluorophenyl)boronic acid (2.84 g, 13.59 mmol) was added in 3 h. After the reaction was completed, the reaction was evaporated under vacuum and applied onto a silica gel column eluted with EA/Hexane (v/v=7/3), the crude was purified by HPLC eluted with CH3CN/H2O (v/v=3/2), this resulted in (784 mg, 38.50%) of 7-(2-amino-3,4,5,6-tetrafluoro-phenyl)-6-chloro-1-(2-isopropyl-4-methyl-3-pyridyl)-4-[(3R)-3-methyl-4-prop-2-enoyl-piperazin-1-yl]-2-oxo-1,8-naphthyridine-3-carbonitrile (P or M isomer, “Compound 9”) as light yellow solid. LCMS: m/z=654 [M+1]+.

1H NMR (400 MHz, CD3OD) δ 8.48 (s, 1H), 8.42-8.31 (m, 1H), 7.23 (d, J=5.0 Hz, 1H), 6.76 (dd, J=16.7, 10.7 Hz, 1H), 6.20 (dd, J=16.7, 1.8 Hz, 1H), 5.73 (dd, J=10.6, 1.9 Hz, 1H), 4.40 (s, 1H), 4.18-4.10 (m, 2H), 3.94-3.83 (m, 2H), 3.65-3.54 (m, 2H), 2.64-2.51 (m, 1H), 2.06-1.92 (m, 3H), 1.44-1.30 (m, 3H), 1.10-1.03 (m, 3H), 0.96-0.81 (m, 3H).

Example 10

4-((R)-4-acryloyl-3-methylpiperazin-1-yl)-7-(2-amino-6-chloro-3,4,5-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Compound 10”); (P)-4-((R)-4-acryloyl-3-methylpiperazin-1-yl)-7-(2-amino-6-chloro-3,4,5-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile; and (M)-4-((R)-4-acryloyl-3-methylpiperazin-1-yl)-7-(2-amino-6-chloro-3,4,5-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile

Step 1. (R)-4-(4-acryloyl-3-methylpiperazin-1-yl)-7-(2-amino-3,4,5-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile

Into a 20-mL round-bottom flask was placed (R)-4-(4-acryloyl-3-methylpiperazin-1-yl)-6,7-dichloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (1.114 g, 2.12 mmol), (2-amino-3,4,5-trifluorophenyl)boronic acid (0.787 g, 4.12 mmol), Pd(PPh3)4(0.527 g, 0.45 mmol), Na2CO3(0.652 g, 6.15 mmol), dioxane (15 mL) and water (3 mL). The reaction mixture was stirred at 80° C. for 1 h. The reaction mixture was filtered and concentrated under vacuum. The residue was purified by Prep-HPLC CH3CN/H2O (0.05% NH4HCO3) (v/v=2/1). This resulted in 1.941 g (69%) of (R)-4-(4-acryloyl-3-methylpiperazin-1-yl)-7-(2-amino-3,4,5-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile as yellow solid. LCMS: m/z=636 [M+1]+.

Step 2. 4-((R)-4-acryloyl-3-methylpiperazin-1-yl)-7-(2-amino-6-chloro-3,4,5-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Compound 10”)

Into a 50-mL round-bottom flask purged and maintained with an inert atmosphere of nitrogen, was placed (R)-4-(4-acryloyl-3-methylpiperazin-1-yl)-7-(2-amino-3,4,5-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (1.904 g, 3.00 mmol), NCS (0.946 g, 6.17 mmol) and AcOH (5 mL). The mixture was stirred at r.t. for 1 d. The residue was purified by Prep-HPLC (CH3CN/H2O)(v/v=6/4). This resulted in 0.497 g (25%) of 4-((R)-4-acryloyl-3-methylpiperazin-1-yl)-7-(2-amino-6-chloro-3,4,5-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Compound 10”) as yellow solid. LCMS: m/z=670 [M+1]+.

Step 3. 4-((R)-4-acryloyl-3-methylpiperazin-1-yl)-7-(2-amino-6-chloro-3,4,5-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (first eluting isomer, “Compound 10-1”) & 4-((R)-4-acryloyl-3-methylpiperazin-1-yl)-7-(2-amino-6-chloro-3,4,5-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (second eluting isomer, “Compound 10-2”)

The mixture of 4-((R)-4-acryloyl-3-methylpiperazin-1-yl)-7-(2-amino-6-chloro-3,4,5-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile atropisomers (495 mg) was purified by Chiral-Prep-HPLC with the following conditions: Column, CHIRAL ART Cellulose-SB, 3 cm×25 cm, 5 um; mobile phase, (Hex:DCM=3:1):EtOH (v/v=90:10); Detection wavelength, UV 220 nm. This resulted in 204 mg (41.21%) of 4-((R)-4-acryloyl-3-methylpiperazin-1-yl)-7-(2-amino-6-chloro-3,4,5-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (the first eluting isomer, “Compound 10-1”) as a yellow solid. LCMS: m/z=670 [M+1]+.

1H NMR (400 MHz, CD3OD) δ 8.58 (s, 1H), 8.41 (dd, J=5.0, 1.4 Hz, 1H), 7.23 (dd, J=8.4, 3.2 Hz, 1H), 6.85 (dd, J=16.7, 10.7 Hz, 1H), 6.29 (dd, J=16.8, 1.8 Hz, 1H), 5.82 (dd, J=10.6, 1.9 Hz, 1H), 4.88 (s, 2H), 4.60 (s, 1H), 4.29-4.22 (m, 1H), 4.09-3.90 (m, 2H), 3.62 (s, 1H), 2.89-2.70 (m, 1H), 1.95 (d, J=12.6 Hz, 3H), 1.45 (d, J=6.5 Hz, 3H), 1.18 (t, J=8.0 Hz, 3H), 1.00 (dd, J=12.5, 6.8 Hz, 3H).

And 170 mg (28.34%) of 4-((R)-4-acryloyl-3-methylpiperazin-1-yl)-7-(2-amino-6-chloro-3,4,5-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (the second eluting isomer, “Compound 10-2”) as a yellow solid. LCMS: m/z=670 [M+1]+.

1H NMR (400 MHz, CD3OD) δ 8.59 (s, 1H), 8.41 (d, J=5.0 Hz, 1H), 7.34-7.17 (m, 1H), 6.85 (dd, J=16.7, 10.7 Hz, 1H), 6.29 (dd, J=16.8, 1.8 Hz, 1H), 5.82 (dd, J=10.6, 1.9 Hz, 1H), 4.88 (s, 2H), 4.60 (s, 1H), 4.27-4.18 (m, 1H), 4.00 (s, 2H), 3.73-3.56 (m, 1H), 2.77-2.49 (m, 1H), 2.05 (d, J=10.4 Hz, 3H), 1.46 (d, J=6.5 Hz, 3H), 1.16 (dd, J=6.8, 1.3 Hz, 3H), 1.06-0.84 (m, 3H).

Example 11

4-((S)-4-acryloyl-2-methylpiperazin-1-yl)-7-(2-amino-3,4,5,6-tetrafluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Compound 11”); (P)-4-((S)-4-acryloyl-2-methylpiperazin-1-yl)-7-(2-amino-3,4,5,6-tetrafluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile; and (M)-4-((S)-4-acryloyl-2-methylpiperazin-1-yl)-7-(2-amino-3,4,5,6-tetrafluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile

Step 1. 4-((S)-4-acryloyl-2-methylpiperazin-1-yl)-7-(2-amino-3,4,5,6-tetrafluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Compound 11”)

Into a 20-mL round-bottom flask was placed (S)-4-(4-acryloyl-2-methylpiperazin-1-yl)-6,7-dichloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (0.311 g, 0.59 mmol), (2-amino-3,4,5,6-tetrafluorophenyl)boronic acid (0.512 g, 1.49 mmol), Pd(PPh3)4(0.085 g, 0.073 mmol), Na2CO3(0.126 g, 1.19 mmol), dioxane (8 mL) and water (2 mL). The reaction mixture was stirred at 80° C. for 1 h. The reaction mixture was filtered and concentrated under vacuum. The residue was purified by Prep-HPLC CH3CN/H2O (0.05% NH4HCO3)(v/v=2/1). This resulted in 135 mg (35%) of 4-((S)-4-acryloyl-2-methylpiperazin-1-yl)-7-(2-amino-3,4,5,6-tetrafluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile as yellow solid (“Compound 11”). LCMS: m/z=654 [M+1]+.

Step 2. 4-((S)-4-acryloyl-2-methylpiperazin-1-yl)-7-(2-amino-3,4,5,6-tetrafluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (first eluting isomer, “Compound 11-1”) & 4-((S)-4-acryloyl-2-methylpiperazin-1-yl)-7-(2-amino-3,4,5,6-tetrafluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (second eluting isomer, “Compound 11-2”)

The mixture of 4-((S)-4-acryloyl-2-methylpiperazin-1-yl)-7-(2-amino-3,4,5,6-tetrafluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile atropisomers (135 mg) was purified by Chiral-Prep-HPLC with the following conditions: Column, CHIRALPAK IF, 2 cm×25 cm, 5 um mobile phase, ((Hex:DCM=3:1):IPA=80:20; Detection wavelength, UV 220 nm. This resulted in 67 mg (49%) of 4-((S)-4-acryloyl-2-methylpiperazin-1-yl)-7-(2-amino-3,4,5,6-tetrafluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (the first eluting isomer, “Compound 11-1”) as a yellow solid. LCMS: m/z=654 [M+1]+.

1H NMR (400 MHz, CD3OD) δ 8.52 (s, 1H), 8.43-8.44 (m, 1H), 7.26 (t, J=5.5 Hz, 1H), 7.02-6.77 (m, 1H), 6.33 (d, J=16.2 Hz, 1H), 5.85 (d, J=10.5 Hz, 1H), 4.67-4.39 (m, 2H), 4.17-4.18 (m, 3H), 3.78-3.56 (m, 2H), 2.81-2.83 (m, 1H), 1.93-1.94 (m, 3H), 1.38-1.40 (m, 3H), 1.18-1.19 (m, 3H), 1.01-1.04 (m, 3H).

And 61 mg (45%) of 4-((S)-4-acryloyl-2-methylpiperazin-1-yl)-7-(2-amino-3,4,5,6-tetrafluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (the second eluting isomer, “Compound 11-2”) as a yellow solid. LCMS: m/z=654 [M+1]+.

1H NMR (400 MHz, CD3OD) δ 8.53 (s, 1H), 8.43-8.44 (m, 1H), 7.29-7.25 (m, 1H), 6.85-6.86 (m, 1H), 6.33 (d, J=15.3 Hz, 1H), 5.85 (d, J=10.7 Hz, 1H), 4.53-4.54 (m, 2H), 4.16-4.17 (m, 3H), 3.65-3.67 (m, 2H), 2.58-2.59 (m, 1H), 2.16-2.02 (m, 3H), 1.38-1.40 (m, 3H), 1.19-1.11 (m, 3H), 0.98-0.99 (m, 3H).

Example 12

4-(4-acryloylpiperazin-1-yl)-7-(3-amino-2-chloro-4,5,6-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Compound 12”); (P)-4-(4-acryloylpiperazin-1-yl)-7-(3-amino-2-chloro-4,5,6-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile; and (M)-4-(4-acryloylpiperazin-1-yl)-7-(3-amino-2-chloro-4,5,6-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile

Step 1. 2,3,4-trifluoro-1-iodo-5-nitrobenzene

Into a 250-mL three-neck bottom flask purged and maintained with an inert atmosphere of nitrogen, was placed 1,2,3-trifluoro-4-nitrobenzene (4.98 g, 28.12 mmol), N-iodosuccinimide (15.99 g, 71.07 mmol), trifluoromethanesulfonic acid (25 mL) and stirred. The resulting solution was stirred overnight at room temperature. The reaction was then quenched by the addition of water (200 mL) slowly. The resulting solution was extracted with EA (2×100 mL), the organic layers were combined, washed with water (100 mL) and brine (100 mL), dried over anhydrous Na2SO4, the residue was concentrated under vacuum. The resulting crude product was further purified by C18column eluted with ACN/H2O (v/v=0%˜100%). This resulted in 4.66 g (54.69% yield) of 2,3,4-trifluoro-1-iodo-5-nitrobenzene.

Step 2. 2,3,4-trifluoro-5-iodoaniline

Into a 250-mL round-bottom flask purged and maintained with an inert atmosphere of nitrogen, was placed 2,3,4-trifluoro-1-iodo-5-nitrobenzene (4.66 g, 15.38 mmol), Fe iron (5.02 g, 89.93 mmol), ammonium chloride (8.57 g, 160.25 mmol), EtOH (30 mL) and water (30 mL). The reaction mixture was stirred at 55° C. for 1 h. The reaction was filtered and the filter cake was washed with EA (2×20 mL). The organic layers were combined and washed with brine (100 mL), dried over anhydrous Na2SO4. The resulting solution was concentrated under vacuum and applied onto a silica gel column eluted with EA/hexane (v/v=1/1). This resulted in 3.69 g (87% yield) of 2,3,4-trifluoro-5-iodoaniline as yellow oil. LCMS: m/z=274 [M+1]+.

Step 3. (5-amino-2,3,4-trifluorophenyl)boronic acid

Into a 250-mL sealed tube purged and maintained with an inert atmosphere of nitrogen, was placed 2,3,4-trifluoro-5-iodoaniline (2.09 g, 7.67 mmol), Bis(pinacolato)diboron (9.46 g, 37.25 mmol), Pd(dppf)Cl2(1.06 g, 1.45 mmol), KOAc (5.10 g, 52.00 mmol) and NMP (80 mL). The reaction mixture was stirred at 95° C. for 2 h. The reaction was then quenched by the addition of saturated sodium carbonate aqueous solution (100 mL). The reaction mixture was stirred at room temperature for 2 h. The resulting solution was extracted with EA (3×100 mL), the organic layers were combined, then washed with brine (100 mL), dried over anhydrous Na2SO4, the residue was concentrated under vacuum. The resulting crude was further purified by C18column eluted with ACN/H2O (v/v=30%˜50%). This resulted in 1.48 g (106% yield) of (5-amino-2,3,4-trifluorophenyl)boronic acid as yellow oil. LCMS: m/z=192 [M+1]+.

Step 4. 4-(4-acryloylpiperazin-1-yl)-7-(5-amino-2,3,4-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methyl pyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile

Into a 40-mL sealed tube purged and maintained with an inert atmosphere of nitrogen, was placed 4-(4-acryloylpiperazin-1-yl)-6,7-dichloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-na phthyridine-3-carbonitrile (0.68 g, 1.34 mmol), (5-amino-2,3,4-trifluorophenyl)boronic acid (0.51 g, 2.67 mmol), Pd(PPh3)4(0.31 g, 0.27 mmol), Na2CO3(0.41 g, 3.86 mmol), dioxane (20 mL) and water (4 mL). The reaction mixture was stirred at 80° C. for 2 h. The reaction was then quenched by the addition of water (50 mL) and ethyl acetate (20 mL). The resulting solution was extracted with ethyl acetate (1×50 mL), the organic layers were combined and dried over anhydrous Na2SO4, filtered and concentrated under vacuum. The resulting crude product was further purified by C18column eluted with ACN/H2O (v/v=0%˜60%). This resulted in 0.15 g (18% yield) of 4-(4-acryloylpiperazin-1-yl)-7-(5-amino-2,3,4-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile as yellow solid. LCMS: m/z=622 [M+1]+.

Step 5. 4-(4-acryloylpiperazin-1-yl)-7-(3-amino-2-chloro-4,5,6-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Compound 12”)

Into a 8-mL sealed tube purged and maintained with an inert atmosphere of nitrogen, was placed 4-(4-acryloylpiperazin-1-yl)-7-(5-amino-2,3,4-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (0.15 g, 0.25 mmol), NCS (0.10 g, 0.77 mmol) and AcOH (4 mL). The mixture was stirred for overnight at room temperature. The reaction was then quenched by the addition of water (50 mL). The resulting solution was extracted with ethyl acetate (2×50 mL), the organic layers were combined and washed with saturated sodium bicarbonate aqueous solution (2×50 mL), dried over anhydrous Na2SO4, filtered and concentrated under vacuum. The resulting crude product was further purified by C18column eluted with ACN/H2O (0.15% NH4HCO3) (v/v=30%˜80%). This resulted in 21 mg (13% yield) of 4-(4-acryloylpiperazin-1-yl)-7-(3-amino-2-chloro-4,5,6-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihyd ro-1,8-naphthyridine-3-carbonitrile (“Compound 12”) as yellow solid. LCMS: m/z=656 [M+1]+.

1H NMR (400 MHz, DMSO-d6) δ 8.57 (s, 1H), 8.44 (d, J=4.7 Hz, 1H), 7.23 (t, J=4.6 Hz, 1H), 6.92 (dd, J=16.7, 10.5 Hz, 1H), 6.20 (dd, J=16.6, 2.3 Hz, 1H), 5.86 (s, 2H), 5.77 (dd, J=10.4, 2.2 Hz, 1H), 4.29-3.50 (m, 8H), 3.72-3.66 (m, 1H), 1.89 (d, J=10.7 Hz, 3H), 1.08 (dd, J=6.6, 2.4 Hz, 3H), 0.97-0.77 (m, 3H).

Step 6. 4-(4-acryloylpiperazin-1-yl)-7-(3-amino-2-chloro-4,5,6-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (first eluting isomer, “Compound 12-1”) & 4-(4-acryloylpiperazin-1-yl)-7-(3-amino-2-chloro-4,5,6-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridin e-3-carbonitrile (second eluting isomer, “Compound 12-2”)

The mixture of 4-(4-acryloylpiperazin-1-yl)-7-(3-amino-2-chloro-4,5,6-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile atropisomers (110 mg, several batches) was purified by Chiral-Prep-HPLC with the following conditions: Column, CHIRAL ART Cellulose-SB, 3 cm×25 cm, 5 um; mobile phase, (Hex:DCM=3:1):EtOH (v/v=95:5); Detection wavelength, UV 220 nm. This resulted in 53 mg (48.18%) of 4-(4-acryloylpiperazin-1-yl)-7-(3-amino-2-chloro-4,5,6-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (the first eluting isomer, “Compound 12-1”) as a yellow solid. LCMS: m/z=656 [M+1]+.

1H NMR (400 MHz, DMSO-d6) δ 8.57 (s, 1H), 8.44 (dd, J=4.9, 1.5 Hz, 1H), 7.24 (t, J=4.8 Hz, 1H), 6.92 (dd, J=16.7, 10.4 Hz, 1H), 6.21 (dd, J=16.6, 2.4 Hz, 1H), 5.88 (s, 2H), 5.77 (dd, J=10.4, 2.4 Hz, 1H), 4.25-2.64 (d, J=27.7 Hz, 8H), 2.78-2.60 (m, 1H), 1.89 (d, J=10.4 Hz, 3H), 1.12-1.01 (m, 3H), 0.97-0.83 (m, 3H).

And 51 mg (46.36%) of 4-(4-acryloylpiperazin-1-yl)-7-(3-amino-2-chloro-4,5,6-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (the second eluting isomer, “Compound 12-2”) as a yellow solid. LCMS: m/z=656 [M+1]+.

1H NMR (400 MHz, DMSO-d6) δ 8.57 (s, 1H), 8.44 (d, J=4.9 Hz, 1H), 7.24 (t, J=4.7 Hz, 1H), 6.92 (dd, J=16.6, 10.4 Hz, 1H), 6.21 (dd, J=16.6, 2.5 Hz, 1H), 5.88 (s, 2H), 5.77 (dd, J=10.3, 2.4 Hz, 1H), 3.89 (d, J=25.8 Hz, 8H), 2.73-2.64 (m, 1H), 1.89 (d, J=10.5 Hz, 3H), 1.08 (dd, J=6.8, 2.6 Hz, 3H), 0.90 (dd, J=19.2, 6.7 Hz, 3H).

Example 13

4-((3S,5R)-4-acryloyl-3,5-dimethylpiperazin-1-yl)-7-(2-amino-3,4,5,6-tetrafluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Compound 13”); (P)-4-((3S,5R)-4-acryloyl-3,5-dimethylpiperazin-1-yl)-7-(2-amino-3,4,5,6-tetrafluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile; and (M)-4-((3S,5R)-4-acryloyl-3,5-dimethylpiperazin-1-yl)-7-(2-amino-3,4,5,6-tetrafluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile

Step 1. 4-((3S,5R)-4-acryloyl-3,5-dimethylpiperazin-1-yl)-7-(2-amino-3,4,5,6-tetrafluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Compound 13”)

Into a 20-mL round-bottom flask was placed 4-((3S,5R)-4-acryloyl-3,5-dimethylpiperazin-1-yl)-6,7-dichloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (0.312 g, 0.58 mmol), 2,3,4,5-tetrafluoro-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)aniline (0.601 g, 2.07 mmol), Pd(pph3)4(0.125 g, 0.11 mmol), Na2CO3(0.189 g, 1.78 mmol), dioxane (8 mL) and water (2 mL).

The reaction mixture was stirred at 80° C. for 1 h. The reaction mixture was filtered and concentrated under vacuum. The residue was purified by Prep-HPLC CH3CN/H2O (0.05% NH4HCO3)(v/v=2/1). This resulted in 0.132 g (34%) of 4-((3S,5R)-4-acryloyl-3,5-dimethylpiperazin-1-yl)-7-(2-amino-3,4,5,6-tetrafluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Compound 13”) as yellow solid. LCMS: m/z=668 [M+1]+.

1H NMR (400 MHz, CD3OD) δ 8.85 (s, 1H), 8.67 (d, J=5.8 Hz, 1H), 7.85 (d, J=5.9 Hz, 1H), 6.94-6.81 (m, 1H), 6.33 (d, J=16.6 Hz, 1H), 5.84 (d, J=10.8 Hz, 1H), 4.78 (s, 2H), 4.09-3.95 (m, 2H), 3.95-3.81 (m, 2H), 3.13-2.99 (m, 1H), 2.33-2.18 (m, 3H), 1.68-1.59 (m, 6H), 1.36-1.24 (m, 3H), 1.23-1.09 (m, 3H).

Step 2. 4-((3S,5R)-4-acryloyl-3,5-dimethylpiperazin-1-yl)-7-(2-amino-3,4,5,6-tetrafluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (first eluting isomer, “Compound 13-1”) & 4-((3S,5R)-4-acryloyl-3,5-dimethylpiperazin-1-yl)-7-(2-amino-3,4,5,6-tetrafluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (second eluting isomer, “Compound 13-2”)

The mixture of 4-((3S,5R)-4-acryloyl-3,5-dimethylpiperazin-1-yl)-7-(2-amino-3,4,5,6-tetrafluoro phenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile atropisomers (930 mg, several batches) was purified by Chiral-Prep-HPLC with the following conditions: Column, CHIRALPAK IA, 3 cm×25 cm, 5 um; mobile phase, Hex:EtOH=80:20; Detection wavelength, UV 220 nm. This resulted in 424 mg (45.59%) of 4-((3S,5R)-4-acryloyl-3,5-dimethylpiperazin-1-yl)-7-(2-amino-3,4,5,6-tetrafluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (the first eluting isomer, “Compound 13-1”) as a yellow solid. LCMS: m/z=668 [M+1]+.

1H NMR (400 MHz, CD3OD) δ 8.83 (s, 1H), 8.52 (d, J=5.3 Hz, 1H), 7.47 (d, J=5.2 Hz, 1H), 6.88 (dd, J=16.7, 10.6 Hz, 1H), 6.33 (dd, J=16.7, 1.9 Hz, 1H), 5.84 (dd, J=10.6, 1.9 Hz, 1H), 4.77 (s, 2H), 4.10-3.94 (m, 2H), 3.91-3.79 (m, 2H), 2.81-2.83 (m, 1H), 2.08-2.10 (m, 3H), 1.65 (t, J=6.0 Hz, 6H), 1.26-1.19 (m, 3H), 1.01-1.02 (m, 3H).

And 372 mg (40.00%) of 4-((3S,5R)-4-acryloyl-3,5-dimethylpiperazin-1-yl)-7-(2-amino-3,4,5,6-tetrafluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (the second eluting isomer, “Compound 13-2”) as a yellow solid. LCMS: m/z=668 [M+1]+.

1H NMR (400 MHz, CD3OD) δ 8.83 (s, 1H), 8.52 (d, J=5.3 Hz, 1H), 7.47 (d, J=5.2 Hz, 1H), 6.88 (dd, J=16.7, 10.6 Hz, 1H), 6.33 (dd, J=16.7, 1.9 Hz, 1H), 5.84 (dd, J=10.6, 1.9 Hz, 1H), 4.77 (s, 2H), 4.10-3.94 (m, 2H), 3.91-3.79 (m, 2H), 2.82-2.83 (m, 1H), 2.09-2.11 (m, 3H), 1.65 (t, J=6.0 Hz, 6H), 1.26-1.19 (m, 3H), 1.04-1.06 (m, 3H).

Example 14

4-((R)-4-acryloyl-3-methylpiperazin-1-yl)-7-(2-amino-3,4,5,6-tetrafluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Compound 14”)

Step 1. 4-((R)-4-acryloyl-3-methylpiperazin-1-yl)-7-(2-amino-3,4,5,6-tetrafluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Compound 14”)

Into a 20-mL sealed tube purged and maintained with an inert atmosphere of nitrogen, was placed (R)-4-(4-acryloyl-3-methylpiperazin-1-yl)-6,7-dichloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (353 mg, 0.67 mmol), 2,3,4,5-tetrafluoro-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)aniline (604 mg, 2.08 mmol), Pd(PPh3)4(185 mg, 0.16 mmol), Na2CO3(235 mg, 2.22 mmol), dioxane (7 mL) and water (0.7 mL). The reaction mixture was stirred at 80° C. for 1 h. The reaction mixture was filtered and concentrated under vacuum. The reaction mixture was concentrated under vacuum. The residue was purified by Prep-HPLC (CH3CN/H2O (v/v=7/3)). This resulted in 29 mg (7% yield) of 4-((R)-4-acryloyl-3-methylpiperazin-1-yl)-7-(2-amino-3,4,5,6-tetrafluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Compound 14”) as yellow solid. LCMS: m/z=654 [M+1]+.

1H NMR (400 MHz, CD3OD) δ 8.50 (d, J=52.4 Hz, 2H), 7.27 (s, 1H), 6.96-6.66 (m, 1H), 6.29 (d, J=16.2 Hz, 1H), 5.83 (d, J=9.6 Hz, 1H), 4.70-4.43 (m, 1H), 4.18-4.08 (m, 4H), 3.70-3.60 (m, 2H), 2.82-2.48 (m, 1H), 2.03 (s, 3H), 1.48-1.40 (m, 3H), 1.24-0.84 (m, 6H).

Example 15

4-((S)-4-acryloyl-2-methylpiperazin-1-yl)-7-(3-amino-2-chloro-4,5,6-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Compound 15”); (P)-4-((S)-4-acryloyl-2-methylpiperazin-1-yl)-7-(3-amino-2-chloro-4,5,6-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile; and (M)-4-((S)-4-acryloyl-2-methylpiperazin-1-yl)-7-(3-amino-2-chloro-4,5,6-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile

Step 1. (S)-4-(4-acryloyl-2-methylpiperazin-1-yl)-7-(5-amino-2,3,4-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile

Into a 20-mL round-bottom flask purged and maintained with an inert atmosphere of nitrogen was placed (S)-4-(4-acryloyl-2-methylpiperazin-1-yl)-6,7-dichloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (0.310 g, 0.59 mmol), (5-amino-2,3,4-trifluorophenyl)boronic acid (0.285 g, 1.49 mmol), Pd(PPh3)4(0.085 g, 0.073 mmol), Na2CO3(0.126 g, 1.19 mmol), dioxane (8 mL) and water (2 mL). The reaction mixture was stirred at 80° C. for 1 h. The reaction mixture was filtered and concentrated under vacuum. The residue was purified by Prep-HPLC CH3CN/H2O (0.05% NH4HCO3)(v/v=2/1). This resulted in 135 mg (35%) of (S)-4-(4-acryloyl-2-methylpiperazin-1-yl)-7-(5-amino-2,3,4-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-m ethylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile as yellow solid. LCMS: m/z=636 [M+1]+.

Step 2. 4-((S)-4-acryloyl-2-methylpiperazin-1-yl)-7-(3-amino-2-chloro-4,5,6-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Compound 15”)

Into a 8-mL sealed tube purged and maintained with an inert atmosphere of nitrogen, was placed (S)-4-(4-acryloyl-2-methylpiperazin-1-yl)-7-(5-amino-2,3,4-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-m ethylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (0.123 g, 193.376 μmol), NCS (0.053 g, 396.906 mol) and AcOH (2 mL). The mixture was stirred for overnight at room temperature. The reaction was then quenched by the addition of saturated sodium bicarbonate aqueous solution (100 mL). The resulting solution was extracted with ethyl acetate (3×100 mL), the organic layers were combined and concentrated under vacuum. The resulting crude product was further purified by C18column eluted with ACN/H2O (0.15% NH4HCO3) (v/v=30%˜80%). This resulted in 55 mg (42% yield) of 4-((S)-4-acryloyl-2-methylpiperazin-1-yl)-7-(3-amino-2-chloro-4,5,6-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Compound 15”) as yellow solid. LCMS: m/z=670 [M+1]+.

1H NMR (400 MHz, CD3OD) δ 8.54 (s, 1H), 8.41 (d, J=4.8 Hz, 1H), 7.24 (dd, J=11.5, 5.9 Hz, 1H), 6.98-6.74 (m, 1H), 6.33 (d, J=16.3 Hz, 1H), 5.84 (d, J=10.5 Hz, 1H), 4.69-3.97 (m, 5H), 3.78-3.52 (m, 2H), 2.86-2.56 (m, 1H), 2.12-1.88 (m, 3H), 1.47-1.30 (m, 3H), 1.25-1.10 (m, 3H), 1.07-0.88 (m, 3H).

Step 2. 4-((S)-4-acryloyl-2-methylpiperazin-1-yl)-7-(3-amino-2-chloro-4,5,6-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (first eluting isomer, “Compound 15-1”) & 4-((S)-4-acryloyl-2-methylpiperazin-1-yl)-7-(3-amino-2-chloro-4,5,6-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (second eluting isomer, “Compound 15-2”)

The mixture of 4-((S)-4-acryloyl-2-methylpiperazin-1-yl)-7-(3-amino-2-chloro-4,5,6-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile atropisomers (53 mg) was purified by Chiral-Prep-HPLC with the following conditions: Column, CHIRALPAK IF, 2 cm×25 cm, 5 um mobile phase, (Hex:DCM=3:1):EtOH (v/v=9:1); Detection wavelength, UV 220 nm. This resulted in 20 mg (49%) of 4-((S)-4-acryloyl-2-methylpiperazin-1-yl)-7-(3-amino-2-chloro-4,5,6-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (the first eluting isomer. “Compound 15-1”) as a yellow solid. LCMS: m/z=670 [M+1]+.

1H NMR (400 MHz, CD3OD) δ 8.56 (s, 1H), 8.43 (d, J=5.0 Hz, 1H), 7.28 (t, J=4.9 Hz, 1H), 6.99-6.79 (m, 1H), 6.35 (d, J=18.0 Hz, 1H), 5.87 (d, J=10.7 Hz, 1H), 4.68-4.06 (m, 5H), 3.78-3.56 (m, 2H), 2.69-2.56 (m, 1H), 2.09 (d, J=9.8 Hz, 3H), 1.42 (s, 3H), 1.17 (d, J=6.8 Hz, 3H), 0.98 (dd, J=18.6, 6.8 Hz, 3H).

And 17 mg (45%) of 4-((S)-4-acryloyl-2-methylpiperazin-1-yl)-7-(3-amino-2-chloro-4,5,6-trifluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (the second eluting isomer, “Compound 15-2”) as a yellow solid. LCMS: m/z=670 [M+1]+.

1H NMR (400 MHz, CD3OD) δ 8.56 (s, 1H), 8.44 (d, J=5.0 Hz, 1H), 7.26 (t, J=5.4 Hz, 1H), 6.87 (d, J=27.5 Hz, 1H), 6.97-6.80 (m, 1H), 5.87 (d, J=11.5 Hz, 1H), 4.65-4.05 (m, 5H), 3.78-3.57 (m, 2H), 2.88-2.78 (m, 1H), 1.95 (d, J=12.5 Hz, 3H), 1.44-1.34 (m, 3H), 1.22 (d, J=6.7 Hz, 3H), 1.03 (dd, J=11.9, 6.8 Hz, 3H).

The following compounds can be synthesized following the similar methods as described in the above-mentioned examples:

EXAMPLE A

4-(4-acryloylpiperazin-1-yl)-7-(2-amino-6-fluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Compound A”)

Step 1. 4,6,7-trichloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile

Into a 50-mL round-bottom flask purged and maintained with an inert atmosphere of nitrogen, was placed 6,7-dichloro-4-hydroxy-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (980 mg, 2.51 mmol), POCl3(1150 mg, 7.50 mmol), DIEA (1.32 g, 10.21 mmol) and acetonitrile (12 mL). The mixture was stirred at 80° C. for 2 h. The reaction was cooled to room temperature and concentrated under vacuum. This resulted in 4,6,7-trichloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile which was used directly in the next step.

Step 2. tert-butyl 4-(6,7-dichloro-3-cyano-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridin-4-yl)piperazine-1-carboxylate

Into a 100-mL round-bottom flask purged and maintained with an inert atmosphere of nitrogen, was placed 4,6,7-trichloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (1.20 g, crude), acetonitrile (20 mL), DIEA (660 mg, 5.10 mmol) and tert-butyl piperazine-1-carboxylate (0.57 g, 3.06 mmol). The reaction mixture was stirred for 2 h at room temperature. The reaction was then quenched by the addition of water (50 mL). The resulting solution was extracted with ethyl acetate (3×50 mL), the organic layers were combined and washed with brine (50 mL), dried over anhydrous Na2SO4, filtered and concentrated under vacuum. The residues was purified by silica gel column eluted with EA/hexane (v/v=30%˜70%). This resulted in 0.92 g (65% in two steps) of tert-butyl 4-(6,7-dichloro-3-cyano-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridin-4-yl)piperazine-1-carboxylate as yellow solid. LCMS: m/z=557 [M+1]+.

Step 3. 4-(4-acryloylpiperazin-1-yl)-6,7-dichloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile

Into a 50-mL sealed tube purged and maintained with an inert atmosphere of nitrogen, was placed tert-butyl 4-(6,7-dichloro-3-cyano-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridin-4-yl)piperazine-1-carboxylate (920 mg, 1.65 mmol), TFA (4 ml) and DCM (15 mL). The reaction mixture was stirred at room temperature for 2 h. The reaction mixture was concentrated under vacuum. The residue was dissolved by DCM (15 mL) in 50-mL round-bottom flask, which was followed by the added of DIEA (1.02 g, 10.08 mmol). The reaction mixture was cooled to 0° C. and acryloyl chloride (190 mg, 2.09 mmol) was added. The mixture stirred at room temperature for 2 h. The reaction was then quenched by the addition of water (30 mL). The resulting solution was extracted with ethyl acetate (3×50 mL), the organic layers were combined and washed with brine (30 mL), dried over anhydrous Na2SO4, filtered and concentrated under vacuum. The residue was purified by silica gel column eluted with EA/hexane (V/V=40%˜80%). This resulted in 0.86 g (crude) of 4-(4-acryloylpiperazin-1-yl)-6,7-dichloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile as yellow solid. LCMS: m/z=511 [M+1]+.

Step 4. 4-(4-acryloylpiperazin-1-yl)-7-(2-amino-6-fluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Compound A”)

Into a 20-mL sealed tube purged and maintained with an inert atmosphere of nitrogen, was placed 4-(4-acryloylpiperazin-1-yl)-6,7-dichloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (99 mg, 0.19 mmol), 3-fluoro-2-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)aniline (78 mg, 0.33 mmol), Pd(PPh3)4(44 mg, 0.03 mmol), Na2CO3(65 mg, 0.61 mmol), dioxane (4 mL) and water (1 mL). The reaction mixture was stirred at 90° C. for 2 h. The reaction mixture was filtered and concentrated under vacuum. The residue was purified by Prep-HPLC CH3CN/H2O (v:v=1/1)). This resulted in 12 mg (10%) of 4-(4-acryloylpiperazin-1-yl)-7-(2-amino-6-fluorophenyl)-6-chloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Compound A”) as yellow solid. LCMS: m/z=586 [M+1]+.

1H NMR (400 MHz, CD3OD) δ 8.52 (s, 1H), 8.46 (d, J=5.0 Hz, 1H), 7.29 (d, J=4.7 Hz, 1H), 7.11 (d, J=6.6 Hz, 1H), 6.89 (dd, J=16.7, 10.6 Hz, 1H), 6.56-6.49 (m, 1H), 6.38-6.30 (m, 2H), 5.90-5.81 (m, 1H), 4.18-3.84 (m, 8H), 2.82-2.70 (m, 1H), 2.08-1.98 (m, 3H), 1.19 (t, J=7.1 Hz, 3H), 1.10-0.93 (m, 3H).

Amgen 6

4-(4-acryloylpiperazin-1-yl)-6-chloro-7-(2-fluorophenyl)-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Amgen 6”)

Step 1. 4-(4-acryloylpiperazin-1-yl)-6-chloro-7-(2-fluorophenyl)-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile

Into a 8-mL sealed tube purged and maintained with an inert atmosphere of nitrogen, was placed 4-(4-acryloylpiperazin-1-yl)-6,7-dichloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-na phthyridine-3-carbonitrile (75 mg, 0.15 mmol), (2-fluorophenyl)boronic acid (56 mg, 0.40 mmol), Pd(dppf)Cl2(25 mg, 34.17 umol), Na2CO3(69 mg, 0.65 mmol), dioxane (1 mL) and water (0.2 mL). The reaction mixture was stirred at 90° C. for 2 h. The reaction mixture was filtered and concentrated under vacuum. The residue was purified by silica gel column eluted with EA/hexane (v/v=7/3). The collecting fluid concentrated under vacuum. The residue was purified by Prep-HPLC CH3CN/H2O (v/v=3/2). This resulted in 22 mg of 4-(4-acryloylpiperazin-1-yl)-6-chloro-7-(2-fluorophenyl)-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Amgen 6”) as yellow solid. LCMS: m/z=571 [M+1]+.

1H NMR (400 MHz, CD3OD) δ 8.52 (s, 1H), 8.43 (d, J=5.0 Hz, 1H), 7.54-7.46 (m, 1H), 7.32-7.13 (m, 4H), 6.89 (dd, J=16.7, 10.6 Hz, 1H), 6.33 (d, J=16.7 Hz, 1H), 5.86 (d, J=10.6 Hz, 1H), 4.15-3.90 (m, 8H), 2.79-2.65 (m, 1H), 2.04 (s, 3H), 1.19 (d, J=6.8 Hz, 3H), 1.00 (d, J=6.7 Hz, 3H).

The mixture of 4-(4-acryloylpiperazin-1-yl)-6-chloro-7-(2-fluorophenyl)-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile atropisomers (1.59 g) was purified by Chiral-Prep-HPLC with the following conditions: Column, CHIRAL Cellulose-SB, 3 cm×25 cm, 5 um; mobile phase, CO2, IPA:ACN=1:1; Detector, UV 254 nm. This resulted in 739 mg (46%) of 4-(4-acryloylpiperazin-1-yl)-6-chloro-7-(2-fluorophenyl)-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (the first eluting isomer, “Amgen 6-1”) as a yellow solid; LCMS: m/z=571 [M+1]+.

And 709 mg (45%) of 4-(4-acryloylpiperazin-1-yl)-6-chloro-7-(2-fluorophenyl)-1-(2-isopropyl-4-methyl pyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (the second eluting isomer, “Amgen 6-2”) as a yellow solid; LCMS: m/z=571 [M+1]+.

1H NMR (400 MHz, CD3OD) δ 8.50 (s, 1H), 8.41 (d, J=5.0 Hz, 1H), 7.52-7.37 (m, 1H), 7.30-7.08 (m, 4H), 6.87 (dd, J=16.8, 10.6 Hz, 1H), 6.38-6.24 (m, 1H), 5.83 (dd, J=10.6, 1.8 Hz, 1H), 4.09-3.82 (m, 8H), 2.78-2.63 (m, 1H), 2.02 (s, 3H), 1.16 (d, J=6.8 Hz, 3H), 0.98 (d, J=6.8 Hz, 3H).

Amgen 6.3

4-(4-acryloylpiperazin-1-yl)-7-(2-amino-6-fluorophenyl)-6-chloro-1-(4,6-diisopropylpyrimidin-5-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Amgen 6.3”)

4-(4-acryloylpiperazin-1-yl)-7-(2-amino-6-fluorophenyl)-6-chloro-1-(4,6-diisopropylpyrimidin-5-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Amgen 6.3”) were prepared according to prior method as yellow solid. LCMS: m/z=615 [M+1]+.

1H NMR (400 MHz, DMSO-d6) δ 9.09 (s, 1H), 8.48 (s, 1H), 7.05 (q, J=7.8 Hz, 1H), 6.93 (dd, J=16.6, 10.4 Hz, 1H), 6.44 (d, J=8.3 Hz, 1H), 6.31 (t, J=8.9 Hz, 1H), 6.21 (dd, J=16.7, 2.4 Hz, 1H), 5.77 (dd, J=10.4, 2.4 Hz, 1H), 5.08 (s, 2H), 3.90 (m, 8H), 2.90-2.74 (m, 1H), 2.70-2.55 (m, 1H), 1.07 (dd, J=12.2, 6.7 Hz, 6H), 1.00 (d, J=6.6 Hz, 3H), 0.86 (d, J=6.7 Hz, 3H).

Amgen 7.3

4-((3S,5R)-4-acryloyl-3,5-dimethylpiperazin-1-yl)-6-chloro-7-(2-fluorophenyl)-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Amgen 7.3”)

Step 1. 4-((3S,5R)-4-acryloyl-3,5-dimethylpiperazin-1-yl)-6-chloro-7-(2-fluorophenyl)-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile

Into a 20-mL sealed tube purged and maintained with an inert atmosphere of nitrogen, was placed 4-((3S,5R)-4-acryloyl-3,5-dimethylpiperazin-1-yl)-6,7-dichloro-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (109 mg, 0.20 mmol), ((2-fluorophenyl)boronic acid (110 mg, 0.79 mmol), Pd(PPh3)4(85 mg, 0.073 mmol), Na2CO3(69 mg, 0.65 mmol), dioxane (6 mL) and water (2 mL). The reaction mixture was stirred at 80° C. for 4 h. The reaction was then quenched by the addition of water (100 mL). The resulting solution was extracted with ethyl acetate (3×100 mL), the organic layers were combined and washed with brine (100 mL), dried over anhydrous Na2SO4, filtered and concentrated under vacuum. The residue was purified by Prep-HPLC (CH3CN/H2O (v/v=1/1). This resulted in 31 mg (26% in two steps) of 4-((3S,5R)-4-acryloyl-3,5-dimethylpiperazin-1-yl)-6-chloro-7-(2-fluorophenyl)-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (“Amgen 7.3”) as yellow solid. LCMS: m/z=599 [M+1]+.

1H NMR (400 MHz, CD3OD) δ 8.80 (s, 1H), 8.44 (d, J=5.0 Hz, 1H), 7.48 (dd, J=8.4, 4.6 Hz, 1H), 7.40-7.22 (m, 4H), 6.98-6.85 (m, 1H), 6.35 (d, J=16.5 Hz, 1H), 5.86 (d, J=10.6 Hz, 1H), 4.79 (s, 2H), 4.14-3.79 (m, 4H), 2.80-2.74 (m, 1H), 2.05 (s, 3H), 1.68 (d, J=7.0 Hz, 6H), 1.19 (d, J=6.8 Hz, 3H), 1.01 (d, J=6.7 Hz, 3H).

The mixture of 4-((3S,5R)-4-acryloyl-3,5-dimethylpiperazin-1-yl)-6-chloro-7-(2-fluorophenyl)-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile atropisomers (1.48 g) was purified by Chiral-Prep-HPLC with the following conditions: Column, CHIRAL Cellulose-SB, 3 cm×25 cm, 5 um; mobile phase, Hex/EtOH=(v/v=50/50); Detection wavelength, UV 254 nm. This resulted in 625 mg (42%) of 4-((3S,5R)-4-acryloyl-3,5-dimethylpiperazin-1-yl)-6-chloro-7-(2-fluorophenyl)-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (the first eluting isomer, “Amgen 7.3-1”) as a yellow solid; LCMS: m/z=599 [M+1]+.

1H NMR (400 MHz, CD3OD) δ 8.68 (s, 1H), 8.32 (d, J=5.0 Hz, 1H), 7.42-7.30 (m, 1H), 7.23-7.01 (m, 4H), 6.78 (dd, J=16.7, 10.6 Hz, 1H), 6.22 (dd, J=16.7, 1.9 Hz, 1H), 5.73 (dd, J=10.6, 1.9 Hz, 1H), 4.66 (s, 2H), 3.99-3.83 (m, 2H), 3.74-3.72 (m, 2H), 2.63-2.61 (m, 1H), 1.93 (s, 3H), 1.55 (d, J=7.0 Hz, 6H), 1.10-1.08 (m, 3H), 0.90-0.89 (m, 3H).

And 669 mg (45%) of 4-((3S,5R)-4-acryloyl-3,5-dimethylpiperazin-1-yl)-6-chloro-7-(2-fluorophenyl)-1-(2-isopropyl-4-methylpyridin-3-yl)-2-oxo-1,2-dihydro-1,8-naphthyridine-3-carbonitrile (the second eluting isomer, “Amgen 7.3-2”) as a yellow solid; LCMS: m/z=599 [M+1]+.

1H NMR (400 MHz, CD3OD) δ 8.80 (s, 1H), 8.43 (d, J=5.0 Hz, 1H), 7.57-7.41 (m, 1H), 7.37-7.07 (m, 4H), 6.90 (dd, J=16.7, 10.6 Hz, 1H), 6.34 (dd, J=16.7, 1.9 Hz, 1H), 5.85 (dd, J=10.6, 1.9 Hz, 1H), 4.78 (s, 2H), 4.07-3.98 (m, 2H), 3.86-3.84 (m, 2H), 2.76-2.74 (m, 1H), 2.05 (s, 3H), 1.68-1.66 (m, 6H), 1.12-1.10 (m, 3H), 0.91-0.89 (m, 3H).

Pharmacological Testing

1. SOS1 Catalyzed Nucleotide Exchange Assay

HIS-KRAS (G12C, aa 2-185, Sino biological) was diluted to 5 μM in EDTA buffer (20 mM HEPES, pH 7.4, 50 mM NaCl, 10 mM EDTA, 0.01% (v/v) Tween-20) and incubated for 30 min at 25° C. The EDTA pretreated HIS-KRAS (G12C) was diluted to 12 nM in assay buffer (25 mM HEPES, pH 7.4, 120 mM NaCl, 5 mM MgCl2, 1 mM DTT, 0.01% (v/v) Tween 20, 0.1% (w/v) BSA) containing 120 nM GDP (Sigma) and MAb Anti 6HIS-Tb cryptate Gold (Cisbio) and incubated for 1 hour at 25° C. to prepare GDP-loaded HIS-KRAS (G12C). The GDP-loaded HIS-KRAS (G12C) was pre-incubation with diluted compounds in a 384-well plate (Greiner) for 1 hour, then purified SOS1 ExD (Flag tag, aa 564-1049) and BODIPY™ FL GTP (Invitrogen) were added to the assay wells (Final concentration: 3 nM HIS-KRAS (G12C), 2 μM SOS1 ExD, 80 nM BODIPY™ FL GTP, 21 ng/mL MAb Anti 6HIS-Tb cryptate Gold) and incubated for 4 hours at 25° C. TR-FRET signals were then read on Tecan Spark multimode microplate reader. The parameters were F486: Excitation 340 nm, Emission 486 nm, Lag time 100 μs, Integration time 200 μs; F515: Excitation 340 nm, Emission 515 nm, Lag time 100 μs, Integration time 200 μs. TR-FRET ratios for each individual wells were calculated by equation: TR-FRET ratio=(Signal F515/Signal F486)*10000. Then the data were analyzed using a 4-parameter logistic model to calculate IC50values. The results of the SOS1 catalyzed nucleotide exchange assay are in the following Table 8:

TABLE 8SOS1 catalyzed nucleotideCompoundexchange IC50(nM)Compound 13.06Compound 1-114.1Compound 1-21.41Compound 26.66Compound 33.67Compound 43.48Compound 55.97Compound 61.51Compound 714.9Compound 86.68Compound 92.30Compound 10-143.9Compound 10-24.16Compound 11-129.6Compound 11-22.11Compound 123.85Compound 12-116.0Compound 12-22.01Compound 136.63Compound 13-12.73Compound 13-211.5Compound 143.56Compound 15-16.87Compound 15-283.9Compound A1.69Amgen 64.40Amgen 6.32.77Amgen 7.310.5

From the Table 8, it can be seen that the representative compounds in the present invention have better activity to inhibit the SOS1 catalyzed nucleotide exchange.

2. Phospho-ERK1/2(THR202/TYR204) HTRF Assay

NCI-H358 cells expressing KRAS G12C were cultured in RPMI 1640 medium (Gibco) containing 10% fetal bovine serum (Gibco). The NCI-H358 cells in culture medium were seeded in 96-well plates at a concentration of 40,000 cells/well and then put in a 37° C./5% CO2cell incubator to incubate overnight. The next day, culture medium was removed and the compound diluted in assay medium (RPMI 1640, 0.1% FBS) was added in each well. After 2 hours incubation in a 37° C./5% CO2cell incubator, the assay medium in 96-well plates was removed, then 50 μL of 1× blocking reagent-supplemented lysis buffer (Cisbio) was added and the plates were incubated at 25° C. for 45 min with shaking. 10 μL of cell lysates from the 96-well plates were transferred to a 384-well plate (Greiner) containing 2.5 μL/well HTRF® pre-mixed antibodies (Cisbio 64AERPEH). Incubate 4 hours at 25° C. and then read HTRF signals on Tecan Spark multimode microplate reader. The data were analyzed using a 4-parameter logistic model to calculate IC50values. The results of the Phospho-ERK1/2(THR202/TYR204) HTRF assay are in the following Table 9:

TABLE 9Compoundp-ERK IC50(nM)Compound 116.4Compound 1-1176Compound 1-210.2Compound 233.6Compound 321.4Compound 440.1Compound 5141Compound 625.1Compound 848.0Compound 926.3Compound 10-1217Compound 10-222.0Compound 11-1258Compound 11-223.6Compound 1239.7Compound 12-1117Compound 12-212.3Compound 1387.0Compound 13-121.8Compound 13-2138Compound 1437.6Compound 15-144.0Compound 15-2327Compound A14.2Amgen 629.9Amgen 6.320.1Amgen 7.344.5

From Table 9, it can be seen that representative compounds in the present invention have better activity to inhibit the phosphorylation of ERK1/2 the NCI-H358 cells.

3. Mouse Pharmacokinetic Study

The purpose of this study was to evaluate the pharmacokinetic properties of compounds in Balb/c mouse (♀) following single dose administration. The day before administration, mice were fasted overnight and free access to water. Six mice were needed for each compound and the six mice were divided into two groups (n=3/group), group A and group B. Mice in group A were treated with a single 3 mg/kg dose of compound (iv). Mice in group B were treated with a single 10 mg/kg dose of compound (po). For each mouse in group A, blood samples were collected at pre-dose, and at the time point of 0.083, 0.25, 0.5, 1, 2, 4, 6, 8 and 24 h post-dose. For each mouse in group B, blood samples were collected at pre-dose, and at the time point of 0.25, 0.5, 1, 2, 3, 4, 6, 8 and 24 h post-dose. Blood samples were placed on ice until centrifugation to obtain plasma samples. The plasma samples were stored at −80° C. until analysis. The concentration of compound in plasma samples were determined using a LC-MS/MS method. The results are in the following Table 10:

TABLE 103 mg/kg, iv10 mg/kg, poCLVssCmaxAUC0-24 hOral BACompound(mL/min/kg)(L/kg)(ng/mL)(ng · h/mL)(F %)Compound 121.41.871397402551.3Compound 117.21.03340958738Compound 139.81.430131276374Compound 14100.940231049863Compound A65.21.132412078.05Amgen 6722.6901103244Amgen 6.3321.41587165832

From Table 10, it can be seen that Compound 1, Compound 11, Compound 13 and Compound 14 have excellent pharmacokinetic properties (such as the higher Cmaxand AUC) in mouse model comparative with the Compound A, Amgen 6 and Amgen 6.3, which make them more suitable for treating cancers with KRAS G12C mutation as an orally therapeutic active ingredient in clinic.

4. Dog Pharmacokinetic Study

The purpose of this study was to evaluate the pharmacokinetic properties of compounds in beagle dog following single dose administration. The day before administration, dogs were fasted overnight and free access to water. Four beagle dogs were needed for each compound and the four dogs were divided into two groups (one male (♂) and one female (♀) in each group). Dogs in group A were treated with a single 1 mg/kg dose of compound (iv). Dogs in group B were treated with a single 10 mg/kg dose of compound (po). For dogs in group A, blood samples were collected at pre-dose, and at the time point of 0.083, 0.25, 0.5, 1, 2, 4, 6, 8 and 24 h. For dogs in group B, blood samples were collected at pre-dose, and at the time point of 0.25, 0.5, 1, 2, 4, 6, 8 and 24 h post-dose. Blood samples were placed on ice until centrifugation to obtain plasma samples. The plasma samples were stored at −80° C. until analysis. The concentration of compound in plasma samples were determined using a LC-MS/MS method. The results are in following Table 11:

TABLE 111 mg/kg, iv10 mg/kg, poCLVSSCmaxAUC0-24 hOral BACompoundSex(mL/min/kg)(L/kg)(ng/mL)(ng · h/mL)(F %)Compound 1-2♂15.90.93420672464.3♀18.11.12980595365.1Compound 9♂17.71.372490778883.2♀20.51.722260632378.5Amgen 6♂11.80.4123690294420.9♀15.50.5432580132612.4Amgen 6.3♂2392.38.05.10.7♀3293.1———

From Table 11, it can be seen that Compound 1-2 and Compound 9 have excellent pharmacokinetic properties (such as the higher Cmaxand AUC) in beagle dog model comparative with the Amgen 6 and 6.3, which make them more suitable for treating cancers with KRAS G12C mutation as an orally therapeutic active ingredient in clinic.

5. Cynomolgus Monkey Pharmacokinetic Study

The purpose of this study was to evaluate the pharmacokinetic properties of compounds in Cynomolgus monkey following single dose administration. The day before administration, monkeys were fasted overnight and free access to water. Four monkeys are needed for each compound and the four monkeys were divided into two groups (one male(♂) and one female (♀) in each group), group A and group B. Monkeys in group A were treated with a single 1 mg/kg dose of compound (iv). Monkeys in group B were treated with a single 3 mg/kg dose of compound (po). For monkeys in group A, blood samples were collected at pre-dose, and at the time point of 0.083, 0.25, 0.5, 1, 2, 4, 6, 8 and 24 h. For monkeys in group B, blood samples were collected at pre-dose, and at the time point of 0.25, 0.5, 1, 2, 4, 6, 8 and 24 h post-dose. Blood samples were placed on ice until centrifugation to obtain plasma samples. The plasma samples were stored at −80° C. until analysis. The concentration of compound in plasma samples were determined using a LC-MS/MS method. The results are in following Table 12:

TABLE 121 mg/kg, iv3 mg/kg, poCLVSSCmaxAUC0-24 hOral BACompoundSex(mL/min/kg)(L/kg)(ng/mL)(ng · h/mL)(F %)Compound 1-2♂5.250.612030793883.5♀7.190.862680685498.9Compound 9♂6.060.6621309697120♀5.730.771060374144.4

From Table 12, it can be seen that Compound 1-2 and Compound 9 have excellent pharmacokinetic properties (such as the higher Cmaxand AUC) in monkey model, which make them more suitable for treating cancers with KRAS G12C mutation as an orally therapeutic active ingredient in clinic.

6. The Efficacy in NCI-H1373 Xenograft Model

NCI-H1373 cells (5.0E+06 cells) were injected subcutaneously into the right flank of female BALB/c nude mice (6-8 weeks) in a mixture with PBS and Matrigel (Corning) (PBS/Matrigel=1:1 (v/v)). Mice were monitored daily and caliper measurements began when tumors became visible. Tumor volume was calculated by measuring two perpendicular diameters using the formula: (L*W2)/2 in which L and W refer to the length and width tumor diameter, respectively. When the average tumor volume reached 150-200 mm3, mice were grouped randomly (n=6/group) and treated with compounds. Tumor volume and mice weight was measured twice a week during treatment (˜ 3 weeks). Tumor growth inhibition rates were calculated by TGI %=(1−(Vt−Vt0)/(Vc−Vc0))*100%, wherein Vc and Vt are the mean tumor volume of control and treated groups at the end of the study respectively, and Vc0and Vt0are the mean tumor volume of control and treated groups at the start respectively. The results are in the following Table 13 andFIG.8:

TABLE 13Tumor volumeTumor volumeat the start,at the endGroupsmm3(Day 21), mm3TGI %Vehicle1931879—Compound 1-2, 10 mg/kg, QD193144103Compound 12-2, 10 mg/kg, QD19363108

From Table 13 andFIG.8, it can be seen that Compound 1-2 and Compound 12-2 have excellent efficacy in vivo.

7. Safety Exploration in MIA PaCa-2 xenograft Model

MIA PaCa-2 cells (1.0E+07 cells) were injected subcutaneously into the right flank of female BALB/c nude mice (6-8 weeks) in a mixture with PBS and Matrigel (Corning) (PBS/Matrigel=1:1 (v/v)). Mice were monitored daily and caliper measurements began when tumors became visible. Tumor volume was calculated by measuring two perpendicular diameters using the following formula: (L*W2)/2 in which L and W refer to the length and width tumor diameter, respectively. After mice were grouped to study the efficacy, the remaining mice (n=6) were used to explore the safety. The mice were treated with 400 mg/kg compound 1-2 (po, QD) for 22 days, and mice body weight was measured twice a week during treatment. The weight of mice varies with the number of days after cell inoculation is shown inFIG.9. FromFIG.9, it can be seen that the Compound 1-2 have good safety.

It is to be understood that, if any prior art publication is referred to herein; such reference does not constitute an admission that the publication forms a part of the common general knowledge in the art in any country. Although the foregoing invention has been described in some detail by way of illustration and example for purposes of clarity of understanding, it is apparent to those skilled in the art that certain minor changes and modifications will be practiced. Therefore, the description and Examples should not be construed as limiting the scope of the invention.