Patent ID: 12209344

DETAILED DESCRIPTION

The present invention will be described in detail through the following embodiments/examples. It should be understood that the specific embodiments are provided for an illustrative purpose only, and should not be interpreted in a limiting manner.

EXAMPLES

Example 1

Table 1 below provides the formulation for incorporating bactericide and virucide into non-woven fabric.

TABLE 1Formulation on non-woven fabric:MaterialsAmountSodium (beta-cyclodextrin)propylsulphonate25 gZinc acetate dihydrate12.5 gPolyvinyl alcohol25 gCitric acid monohydrate25 gDistilled water1162.5 g
Preparation Procedure:1. Polyvinyl alcohol (2% wt against water) was first dissolved in water at 80 degrees Celsius with stirring until it was fully dissolved. The solution was then cooled down to 25 degrees Celsius and citric acid monohydrate (2% wt against water), sodium (beta-cyclodextrin)propylsulphonate (2% wt against water) and zine acetate dihydrate (1% wt against water) were added into the solution at 25 degrees Celsius. The mixture was then stirred at 25 degrees Celsius until all materials were dissolved;2. Non-woven fabric (e.g., PE or PP) was dipped into the mixture obtained from 1 and completely immersed therein for 5 minutes at 25 degrees Celsius under stirring to make the fabric soaking wet;3. The wet fabric was dried at 120 degrees Celsius for 10 minutes.
A coating GSM of about 13.0 to 20 g/m2was resulted in the fabric after drying.

Below summarizes the bactericidal activity (Table 2) and virucidal activity (Table 3) of the non-woven fabric incorporated with the formulation 1 and the procedure described in this example, using the test standards ISO 20743 and ISO 18184, respectively, with methods that are known in the state-of-the-art

TABLE 2Bactericidal Activity:BactericidalTest StrainContact TimeActivity (Log10)Example 1Staphylococcus2 h>4.79aureusKlebsiella>5.71pneumonia

From Table 2, the bactericidal activity of the PP non-woven fabric of the Example 1 is reflected by the bactericidal activity (Log10) over a 24-hour test.

TABLE 3Virucidal Performance:Virucidal ActivityTest StrainContact Time(Log10TCID50/mL)Example 1H1N1 A/PR8/345min3.732h6.56H3N25min3.112h4.89SARS-CoV-15min1.25

From Table 3, it shows that the PP non-woven fabric incorporated with the formulation 1 is effective in reducing the viral titer of different strains (herein include H1N1 A/PR8/34, H3N2 and SARS-CoV-1) within 2-hour test. When comparing the two viral strains, it seems that the PP non-woven fabric incorporated with formulation 1 is more effective in reducing the titer of H1N1 A/PR8/34 strain than that of H3N2, but it is not conclusive until more viral strains are tested. Overall, the fabric incorporated with the formulation 1 has virucidal activity against certain commonly known viruses, including coronavirus SARS-CoV-1.

Example 2

Table 4 below provides the formulation for incorporating bactericide and virucide into non-woven fabric.

TABLE 4Formulation on non-woven fabric:MaterialsAmountSodium (beta-cyclodextrin)propylsulphonate25gZinc acetate dihydrate12.5gCopper(II) acetate hydrate12.5gPolyvinyl alcohol25gCitric acid monohydrate25gDistilled water1150g
Preparation Procedure:1. Polyvinyl alcohol (2% wt against water) was first dissolved in water at 80 degrees Celsius with stirring until it was fully dissolved. The solution was then cooled down to 25 degrees Celsius and citric acid monohydrate (2% wt against water), sodium (beta-cyclodextrin)propylsulphonate (2% wt against water) and zine acetate dihydrate (1% wt against water) and copper(II) acetate hydrate (1% wt against water) were added into the solution at 25 degrees Celsius. The mixture was then stirred at 25 degrees Celsius until all materials were dissolved;2. Non-woven fabric (e.g., PE or PP) was dipped into the mixture obtained from 1 and completely immersed therein for 5 minutes at 25 degrees Celsius under stirring to make the fabric soaking wet;3. The wet fabric was dried at 120 degrees Celsius for 10 minutes.
A coating GSM of about 13.0 to 20 g/m2was resulted in the fabric after drying.

Below summarizes the virucidal activity (Table 5) of the non-woven fabric incorporated with the formulation and the procedure described in this example against coronaviruses, using the test standards ISO 18184, respectively, with methods that are known in the state-of-the-art

TABLE 5Virucidal Performance against Coronaviruses:Virucidal ActivityTest StrainContact Time(Log10TCID50/mL)Example 2HCoV-229E5 min2.50SARS-CoV-15 min2.73

From Table 5, it shows that the PP non-woven fabric incorporated with the formulation is effective in reducing the viral titer of different strains of coronaviruses HCoV-229E (Human Coronavirus) and of SARS-CoV-1 (responsible for the pandemic outbreak in Asia in 2003) within contact time of 5 minutes.

Example 3

Below illustrates the formulation and procedure in two parts for incorporating bactericide into a cotton woven fabric

TABLE 6Formulation for Cotton Fabric - Part I:MaterialsAmountBeta-cyclodextrin-containing compound associated22gwith zinc ions and ethylenediaminetetraacetic aciddescribed in the third embodimentCitric acid100gSodium dihydrogen phosphate30gDistilled water1L
Procedure I:1. All of the solid reagents were dissolved in water at 60 degrees Celsius to obtain a first solution;2. A piece of cotton fabric was dipped into the first solution for 5 minutes at 60 degrees Celsius;3. The fabric was padded, and dried in an oven at 90 degrees Celsius for 10-20 minutes, then at 120 degrees Celsius for 20 minutes;4. The dried fabric was rinsed with water, padded dry again.

TABLE 7Formulation for Cotton Fabric - Part II:MaterialsAmountSodium hydrogen carbonate50gZinc acetate60gDistilled water1L
Procedure II1. Sodium hydrogen carbonate was dissolved in 1 liter distilled water at room temperature to obtain a second solution;2. Cotton fabric from Part I was immersed into the second solution for 15 to 20 minutes;3. The wet fabric was rinsed with distilled water, padded dry;4. Zinc acetate was dissolved in 1 liter distilled water in another container at room temperature to obtain a third solution;5. Cotton fabric from step 3 was immersed into the third solution for 10 minutes;6. The fabric was rinsed with water, padded dry again, and dried in oven at 90 degrees Celsius for 10-20 minutes;
A coating GSM of approximately 15 g/m2was resulted from the cotton fabric incorporated with the formulation in Example 2.

Below (Table 6) illustrates the durability of the bactericidal performance of cotton fabric incorporated with the formulation described in Example 2 in terms of the difference in bacterial count reduction before and after 30 cycles of laundry washing and tumble dry, tested using the methods described in AATCC 100.

TABLE 8Bactericidal Performance:EffectiveEffective Bacterial CountBacterial CountReduction After 30 HomeContactReductionLaundry Washing andTest StrainTime(Log10)Tumble Dry Cycles (Log10)Staphylococcus24 h3.03.0aureusKlebsiella3.23.2pneumoniaEscherichia coli3.33.3

From Table 8, it shows that the effective bacterial count reduction in three different bacterial strains after 30 cycles of home laundry washing and tumble dry remains unchanged. It can be concluded that the bactericidal activity of the woven fabric prepared according to the formulation and procedures in this example is durable even it is subjected to repeated washing and tumble dry, mimicking the situation of daily use of the products made of this kind of fabric.

INDUSTRIAL APPLICABILITY

The present invention is applicable in textile and disposable wearables including but not limited to specialized wearables and gears, medical device and equipment, which require antibacterial and/or antiviral functions.