Patent ID: 12216264

In the accompanying drawings:1—laser;2—half-wave plate;3—quarter-wave plate;4—one-dimensional scanning galvanometer;5—scanning lens;6—tube lens;7—array vortex wave plate;8—non-polarizing beam splitter;9—objective lens;10—sample to be detected;11three-dimensional moving stage;12—aperture diaphragm array;13—focusing lens;14—single-mode optical fiber; and15—PMT detector.

DETAILED DESCRIPTIONS OF THE EMBODIMENTS

Exemplary embodiments of the present disclosure will be described below in conjunction with the accompanying drawings. For the sake of clarity and conciseness, not all features of the actual implement are described in the description. However, it should be understood that many implementation-specific decisions must be made in the process of developing any such practical embodiment, so as to achieve specific objectives of the developers, such as meeting those restrictions associated with the system and the business, and those restrictions vary with the implementation. It should be also understood that although the development work is likely to be very complex and time-consuming, such development work is merely a routine task for those skilled in the art who benefit from the disclosure of the present disclosure.

It should be further noted that, in order to avoid obscuring the present disclosure due to unnecessary details, only the structure and/or processing steps closely related to the solution according to the present disclosure are shown in the accompanying drawings, while other details that have little to do with the present disclosure are omitted.

Embodiment 1

As shown inFIG.1, the present embodiment provide a dark-field confocal microscopic measurement apparatus based on vortex dichroism, which is configured for realizing a dark-field chiral measurement function of samples.

The apparatus includes an array vortex light generation module, an array vortex light illumination module and an array dark-field confocal detection module;the array vortex light generation module generates annular light illumination, and includes the following components in sequence according to a direction of light propagation: a laser1, a half-wave plate2, a quarter-wave plate3, a one-dimensional scanning galvanometer4, a scanning lens5, a tube lens6and an array vortex wave plate7, a vortex illumination light beam is generated and irradiated to the array vortex light illumination module via a non-polarizing beam splitter8;the laser1emits linearly polarized laser, a state of polarization is regulated by the half-wave plate2and the quarter-wave plate3, and the one-dimensional scanning galvanometer4, the scanning lens5and the tube lens6make the light beam pass through centers of different-order vortex phases of the array vortex wave plate7to generate an annular vortex illumination beam;the array vortex light illumination module includes the following components in sequence according to a direction of light propagation: the non-polarizing beam splitter8, an objective lens9, a sample to be detected10and a three-dimensional moving stage11, where the objective lens9focuses a light beam on the sample to be detected10placed on the three-dimensional moving stage11, and feeds back reflected light and scattered light to the array dark-field confocal detection module;the array dark-field confocal detection module includes the following components in sequence according to a direction of light propagation: an aperture diaphragm array12, a focusing lens13, a single-mode optical fiber14and a PMT detector15, and the feedback scattered light is collected to obtain vortex dichroic scattering signals; andthe objective lens9collects the reflected light and the scattered light of the sample, blocks the annular reflected light through the aperture diaphragm array12, and the focusing lens13focuses the scattered light to the single-mode optical fiber14and records the scattered light by the PMT detector15.

More specifically, the laser1emits the linearly polarized laser, and a polarization direction of a light beam is regulated to circularly polarized light by the half-wave plate2and the quarter-wave plate3.

More specifically, the light beam is changed in a propagation direction by the one-dimensional scanning galvanometer4, irradiates to the array vortex wave plate7after passing through the scanning lens5and the tube lens6, the array vortex wave plate7includes a vortex phase array, each vortex phase distribution exp (imφ) corresponds to an order m, the light beam irradiates on the vortex phase array to generate m-order vortex illumination light beams, and the light beam is incident on centers of ±m-order vortex phase diagrams, where m=0, 1, 2 . . . and 10.

More specifically, after passing through the non-polarizing beam splitter8, vortex illumination light beams at various orders passing through the array vortex wave plate7are focused on the same focal point on the sample to be detected10by the objective lens9, and reflected light and scattered light collected by the objective lens9are refracted to the aperture diaphragm array12through the non-polarizing beam splitter8.

More specifically, a center position of each clear aperture on the aperture diaphragm array12is the same as that of a vortex phase of the array vortex wave plate7, clear apertures are the same, and the specific aperture size is matched with a central dark spot of the annular vortex light reflected under the illumination of 1-order vortex illumination beams.

More specifically, the scattered light passing through the aperture diaphragm array12enters the focusing lens13, and couples into the single-mode optical fiber14, and the PMT detector15collects scatted light signals and records signal strength.

More specifically, differences between PMT signals collected by the PMT detector15under the illumination of a +m-order vortex illumination light beam and PMT signals collected by the PMT detector15under the illumination of a −m-order vortex illumination light beam are identified to obtain the vortex dichroic scattering signals.

Embodiment 2

The present embodiment provide a dark-field confocal microscopic measurement method based on vortex dichroism, which is adopted for realizing dark-field confocal detection and chiral detection functions of samples. The method includes the following specific steps:step a, emitting linearly polarized laser from the laser1, and regulating the polarization direction of the light beam to circularly polarized light by the half-wave plate2and the quarter-wave plate3;step b. changing the propagation direction of the circularly polarized light through the one-dimensional scanning galvanometer4, and loading 21 fixed voltages on the one-dimensional scanning galvanometer4, so that the deflected light beam, after passing through the scanning lens5and the tube lens6, is separately incident on centers of ±m-order vortex phase diagrams, where m=0, 1, 2 . . . and 10, and vortex illumination beams are generated;step c. having the vortex illumination light beams that pass through the array vortex wave plate7after passing through the non-polarizing beam splitter8focused on the same focal point on the sample to be detected10by the objective lens9;step d. filtering out random-order vortex reflected light through the aperture diaphragm array12after reflected light and scattered light collected by the objective lens9pass through the non-polarizing beam splitter8, and retaining the scattered light in a center of the light beam;step e, focusing the scattered light to the single-mode optical fiber14by the focusing lens13, and collecting the scattered light by the PMT detector15;step f. identifying differences between PMT signals recorded by the PMT detector15under the illumination of ±m-order vortex illumination light beams to obtain vortex dichroic scattering signals; andstep g. moving the sample to be detected10by using the three-dimensional moving stage11, changing the position of a focused light spot, and returning to the step c to obtain vortex dichroic scattering signals of the position of the next focused light spot.

More specifically, the laser beams emitted by the laser1have a wavelength of 400 nm-620 nm.

Although the implementation disclosed in the present disclosure is described as above, the contents thereof are only the implementation adopted to facilitate the understanding of the technical solutions of the present disclosure, and are not intended to limit the present disclosure. Those skilled in the art to which the present disclosure belongs may make any modifications and changes in the form and details of the implementation without departing from the core technical solution disclosed in the present disclosure, but the scope of protection limited by the present disclosure shall still be subject to the scope limited by the appended claims.