Patent ID: 12228503

DETAILED DESCRIPTION

The following description of example methods and apparatus is not intended to limit the scope of the description to the precise form or forms detailed herein. Instead the following description is intended to be illustrative so that others may follow its teachings.

Mid-infrared radiation is resonant to the vibrational excitations of many chemical bonds and can, therefore, its absorption spectra reveal a wealth of information of molecular composition. When MIR spectroscopy is performed in a localized area, identifying, characterizing and tracking microscopic objects become accessible. Databases of MIR spectra enable analysis of complex structures, such as single live cells and individual protein complexes. Since MIR spectroscopy reflects the vibrations of chemical bonds, the unique combination of different chemical bonds in a molecule gives rise to a characteristic spectral “fingerprint.” This circumvents the common procedures of applying stains, fixatives, or exogenous fluorophores for imaging biological samples.

Although MIR spectroscopy is a powerful tool, there are two major inherent drawbacks. First, MIR imaging typically has a limited spatial resolution. According to Abbe diffraction, the minimum spatial resolution that can be achieved is

resolution=λ2*NA(1)
where λ denotes the wavelength of light and NA denotes the numerical aperture of the objective. Thus, MIR radiation typically has a spatial resolution that is around an order-of-magnitude worse than visible light, given the same or similar optics conditions.

In order to solve the problems described above, a technique that uses an affordable and reliable detector and has a superior spatial resolution is needed. Mid-infrared photothermal heterodyne imaging (MIR-PHI) systems overcome the diffraction limit of traditional MIR imaging. Photothermal imaging systems of the present application utilize a combination of a mid-IR “pump” laser and a visible light “probe” laser. Under one theory of operation, irradiating a sample with the pump laser may heat the sample and its surrounding medium, which can cause a thermal “lens” to form.

The photothermal effect is the phenomenon that, when an object absorbs photons, it can release the energy through radiating heat to its surrounding medium. This forms a temperature gradient in the medium around the object. Then by using a separate probe light source with a much shorter wavelength than the MIR wavelengths (i.e., at a visible or near-IR wavelengths), the MIR-PHI can measure mid-IR absorption of the sample with a spatial resolution well below the Abbe diffraction limit of the mid-IR pump beam. The use of visible or near-IR wavelength for the probe beam enables use of visible/near IR photodiodes as detectors disclosed herein that may accomplish using reliable and cost-effective detectors with higher sensitivity than mid-IR detectors. Since the refractive index of the medium is related to its temperature, the temperature gradient creates a refractive index gradient which resembles a lens. This thermal lens can then affect the propagation of a probe beam and thus generate a photothermal signal. Raster scanning in two dimensions—using a movable platform such as a piezo stage—can create a contrast between the presence and absence of the thermal lens, thereby forming a photothermal image.

According to one embodiment, a pump beam is at least partially absorbed by an analyte or sample, which gives rise to localized heating. The heating effect can cause the refractive index of the particle and its surrounding environment to change, creating a refractive index gradient. This refractive index gradient in the medium behaves in a similar way as a lens, and is referred to as a “thermal lens.” The probe beam—which is preferably non-resonant to the analyte's absorption—is then used to detect the thermal lens effect. A shorter wavelength probe can be utilized, so that the Abbe diffraction resolution is much improved. In some embodiments the probe wavelength can be selected in the visible region, where reliable, sensitive and affordable detectors are readily available. Furthermore, the power of the probe can be operated at a higher power levels, in order to minimize the photon noise, as long as the power level is below the sample damage threshold.

Photothermal imaging offer several advantages that solve the problems associated with conventional mid-IR imaging. For the scarcity of detector issue, since the photothermal effect a visible or near-infrared light source, such as a visible or near IR laser can be used as the probe. Detectors for visible or near-infrared light can be faster, cheaper and more sensitive. Also, the spatial resolution of photothermal imaging is determined by the probe wavelength (not the wavelength of the mid-IR heating beam), which implies a significant improvement over the spatial resolution of conventional mid-IR imaging. This can be very useful in many applications, for instance in the medical imaging field, because it can potentially push the limit of mid-IR imaging to a subcellular level or even smaller, while the commonly used scale right now is at the level of imaging tissues. On the other hand, using a mid-IR pump beam in the photothermal technique also remedies one of the shortcomings of conventional photothermal imaging measurements. Since the photothermal effect is based on the absorption of photons, the conventional photothermal imaging measurements using visible or near-IR pump lasers are limited to studying materials such as noble metal nanoparticles and dye molecules that absorb in the visible region. Since almost all compounds absorb mid-IR light and then generate thermal energy, the applicability of photothermal imaging is vastly broadened by the introduction of the mid-IR technique.

In this disclosure, PHI experiments are described that utilize a mid-IR pump laser source. It is shown that MIR-PHI imaging systems can achieve micrometer spatial resolution, which is much smaller than the diffraction limit of the mid-IR light source. The combination of mid-IR and photothermal imaging techniques exploits affords the benefits of improved spatial resolution with robust and inexpensive detectors, while also maintaining the spectroscopic potential associated with mid-IR absorption.

In summary, MIR-PHI systems overcome the diffraction limit typically inherent to MIR imaging, while using fast and accurate visible/near IR photo detectors. With our counter-propagating geometry, it is possible to obtain high contrast images of 1.1 μm polystyrene beads in glycerin and in air. Clear absorption spectra were also acquired locally on the single particles. These improvements to mid-IR imaging have the potential to provide opportunities for researchers to investigate biological systems in media on a spatial scale that was not previously achievable, and thus has broad application potential in biological imaging.

FIG.1is a schematic diagram of an example photothermal imaging system100. In photothermal imaging system100, a probe laser110emits a probe beam111toward a probe mirror112, which reflects the probe beam111onto beam splitter113. Beam splitter113may be a polarizing beamsplitter or similar to allow the probe beam111reflecting off the probe mirror112to pass through to the objective114and onto a first side of sample130. In some implementations, the probe beam111is a visible light laser beam, and/or a near IR laser beam.

The pump laser150emits a pump beam151toward a pump mirror152, which reflects pump beam151toward a reflective objective153The reflective objective153may serve to irradiate the sample130from a second side, which is opposite to the first side. This arrangement—in which the probe beam111and pump beam151illuminate or irradiate the sample130from opposite sides—may be referred to herein as a “counter propagating geometry.” In some implementations, the pump beam151may be a mid-IR laser beam.

During operation, the pump beam151irradiates the sample130, which absorbs the mid-IR light, causing localized heating on the sample130. This localized heating can lead to the thermal lens effect described herein. The probe beam111incident on the sample130interacts with a region of the sample illuminated by the pump beam. which scatters some of the light of probe beam111, for example due to the thermal lens temperature gradient of an absorbing region of the sample Some of the reflected probe beam111then travels back toward the beam splitter113, which directs the reflected probe beam111toward a photodiode122. The photodiode122then collects and measures the probe beam light111, which may be stored on a computing device or the like.

The step of mid-IR irradiation, and probe beam reflection measurement by the photodiode122may be repeated at different locations on sample130. Movable platform140—which may be a base controlled by servos, a piezo stage, or other actuating platform—may move the sample130laterally or longitudinally (with respect to the objective114) in order to position objective114and reflective objective153over a different location on sample130. That new location may then be irradiated by the mid-IR pump beam151, and measured by the reflected probe beam111incident on photodiode122. By repeating this step at various locations of the sample130and storing the read out values from photodiode122, a computing device may then construct a 2D image representing the photothermal intensities of the sample.

The photodiode122may provide a probe signal input indicative of the intensity of the reflected probe beam111to a lock-in amplifier120. The lock-in amplifier120may be configured on the basis of the wavelength of the probe beam111(i.e., referenced to the modulation frequency of the pump beam) and may be capable of detecting minor fluctuations in the properties of the probe beam111caused by the thermal lens effect of the sample130. The lock-in amplifier120may also receive a pump beam modulation signal123from the pump laser150indicative of the modulation frequency of the pump beam151(e.g., 100 kHz, 150 kHz, etc.). In some implementations, the lock-in amplifier120also receives a signal from the probe laser110indicative of the frequency or wavelength of the probe beam111.

An example MIR-PHI system (such as the photothermal imaging system100shown inFIG.1) comprises two laser sources. A tunable MIR pump laser150(e.g., with wavelengths between 2.5-3.7 μm), operating under pulse mode at 150 kHz, is used as the pump laser source. This range covers the C—H, N—H, and O—H stretch absorption peaks, which are present in organic compounds. A tunable pump light source enables the measurement to be made a multiple mid-IR wavelengths, thereby allowing for data to be taken that is representative of an IR absorption spectrum at sub-micron spatial resolution. For some of the experiments described below, the pump laser is tuned to 3030 cm−1to excite 1.1 μm polystyrene beads. A counter-propagating, continuous wave (CW) 532 nm probe laser110is used to detect the thermal lens created by the pump laser150. The pump laser150is focused by a reflective objective153(NA=0.65), and the probe beam111is focused by a long working distance, high NA glass objective114(NA=0.80). This arrangement is different to that used in previous MIR-PHI measurements, where a single reflective objective was used to focus the pump and the probe at the sample. The advantage of this setup is that a tighter focus can be obtained for the probe beam111, yielding higher spatial resolution. A disadvantage is that two counter-propagating beams have to be carefully aligned with each other to maximized the PHI signal. The reflected probe beam is detected by a Si-photodiode, and the signal from the Si-photodiode is sent to a lock-in amplifier (e.g., a Stanford Research SR-844 RF Lock-in Amplifier), which is triggered by pulses from the MIR laser at 150 kHz. Raster scanning with a piezo stage140across an area on the order of 10×10 μm therefore results in a MIR-PHI image.

In terms of the sample preparation procedure, an optical cell with high mid-infrared transmission (e.g., two sapphire windows) can be used. Specimens are applied on a surface of one of the sapphire windows by drop-casting or spin-coating. A small amount of medium can be dropped onto the specimen. Parafilm or a sealant can then be used to seal the peripheral areas of the two windows together, and thus create an optical cell with a thin chamber of the specimen surrounded by the solvent. The thickness of the sapphire window was selected to be small, so that it will be within the working distance of the objective.

The probe beam111, which can be a visible or near-infrared laser, may be operated in the continuous wave (CW) mode. In one embodiment, the two lasers may be designed to be counter-propagating whereby the sample is illuminated from different sides by the two lasers. This design avoids the usage of dichroic mirrors for combining the mid-IR and visible light, which may be desirable due to the dichroic mirrors for these two laser wavelengths vastly attenuating the intensity of the mid-IR beam. This arrangement also allows the use of high magnification microscope objectives for the probe beam111(since objectives that transmit IR light are typically low magnification).

In one embodiment, the probe beam111is directed at a first sample surface while the pump beam151is directed to a second sample surface opposite the first. In other embodiments, the surfaces illuminated by the two beams may not be exactly opposite one another. In one instance, the pump beam151may be directed to an adjacent surface as the one being illuminated by the probe beam111. In another embodiment, the pump beam151may be directed to the same area where the surface illuminated by the probe beam111resides. In one embodiment, the angle of separation between the pump beam151and the probe beam111may be about 180 degrees. In other embodiments, the angle of separation may be some angle less than 180 degrees but sufficient to provide a separate pathway for the pump beam151and probe beam111. The angle of separation provides two pathways, one for the probe beam111and one for the pump beam151to illuminate the same area of the sample under test.

In one embodiment, the reflected probe beam from the sample surface is then collected by the same objective used to focus it, and detected by a photo detector, which can simply be a regular visible light photodiode (e.g., Si, InGaAs, Ge, etc.). The signal from the photo detector122is then processed by a lock-in amplifier120, referenced to the modulation frequency (via signal123) of the pump beam151. This allows the lock-in amplifier120to detect the amplitude and the relative phase of the photothermal signal output from the photodiode122. A raster scan of the sample over a certain area, which can be conducted by a piezo-stage140, then creates a mid-IR photothermal image.

Signal processing devices, such as the lock-in amplifier, may sample or measure one or more property of collected probe light (i.e., reflecting off a sample or a region of a sample). For instance, a signal processing device may—based on parameters of the photothermal imaging system (e.g., a pump beam pulse or modulation frequency, a wavelength of the pump beam, a wavelength of the probe beam, etc.)—determine a frequency, shift in frequency, phase, phase shift, intensity, or any other property of the collected probe beam light. The property or properties measured and/or determined by the signal processing device may serve as a basis for analyzing, imaging, conducting spectroscopic analysis, and/or other operations on a sample or a region of a sample.

In order to obtain good imaging contrast, the selection of the mid-infrared wavelength is important. If the analyte has an absorption peak at a certain wavelength while the solvent doesn't absorb at that same wavelength, the contrast of the image will be high. This can be achieved by using solvents with limited mid-infrared absorption features, such as CS2and CCl4. For water—which has a very broad absorption in the mid-IR region—two strategies can be used to obtain an image with good contrast. First, images can be captured at a wavelength where the absorption of water is limited and the absorption from the analyte is at its maximum. For example, at 2850 cm−1, a polystyrene bead can be clearly imaged with the mid-PHI technique in water (as shown inFIG.2A). Another method involves tuning the mid-IR wavelength to water's absorption peak while the analyte doesn't have any absorption. For example, at 3300 cm−1, a clear image can also be collected for polystyrene beads in water (shown inFIG.2B). The two images of the same object using these two strategies will be complementary with each other, one with a dark background and a bright signal, and the other one with a bright background and a dark signal.

The solvent used in the photothermal imaging system can also affect its performance. Some organic solvents have outstanding photothermal properties, such as CS2and CCl4. Photothermal imaging with these solvents results in a very high signal-to-noise sensitivity. However, they are usually toxic, volatile, and not compatible with biological samples. Glycerin, which is biocompatible and viscous, has a relatively high photothermal performance. Water, as the ideal medium for biological samples, does not have a sensitive photothermal response compared to other solvents. However, water was used to achieve a visualization of one micron polystyrene particles in water at a single particle level using this technique. For example, 1.1 μm polystyrene beads were successfully visualized with super-resolution in water (as shown inFIG.2AandFIG.2B).

The selection of medium is an important factor for PHI measurements. A preferable medium for PHI includes the following characteristics. First, a large refractive index provides for better performance of the thermal lens. Second, a preferable PHI medium has a small heat capacity, with the aim to maximize the change in temperature created by a given amount of heat transferred from the particle. Additionally, the more sensitive the refractive index is to temperature, the shaper the refractive index gradient will be in the thermal lens. A figure-of-merit for the overall photothermal performance of a medium can be expressed with equation (2) below:

ΣPhotothermal=n⁢❘"\[LeftBracketingBar]"δ⁢nδ⁢T❘"\[RightBracketingBar]"⁢(1CT)(2)
where n denotes the refractive index, T denotes the temperature, and CTrepresents the heat capacity under temperature T. Researchers have compared the performance of different common solvents. Among them, carbon disulfide (CS2) was found to have preferable photothermal properties. Additionally, carbon tetrachloride, chloroform, hexane, decane, ethanol, glycerin, and water are other solvents that exhibit preferable photothermal properties.

The thermal diffusion length is another factor that has a large impact on PHI image contrast. Since the particles are heated by the pump beam intermittently at the modulation or pulse frequency, the magnitude of the frequency is directly related to the thermal diffusion length. Lower frequencies give longer thermal diffusion lengths and yield higher photothermal signals, until the thermal diffusion length exceeds the size of the focal spot. Therefore a focal spot size that matches the thermal diffusion length will be optimal. The thermal diffusion length (rth) of the medium can be calculated with the equation (3) below:

rth2=2⁢DthΩ=2⁢kCp⁢Ω(3)

where Dthis the thermal diffusivity, k is the thermal conductivity of the medium, Ω is the modulation frequency, and Cpis the heat capacity per unit volume photothermal medium. At the same time, the lower the modulation frequency, the longer the thermal diffusion length, and the worse the spatial resolution will be. That is why we triggered our measurement at the high pulse frequency of 150 kHz, which is much higher than that in the previous literatures (20 Hz). This made sub-diffraction limit resolution (of the pump beam) practically plausible. The thermal diffusion length (rth) of different media are calculated. Under 150 kHz of modulation, carbon disulfide (CS2) has a diffusion length of 1.3 μm, that of glycerin is 1.2 μm, and that of water is 1.3 μm. A 532 nm probe laser, the size of the focal spot is estimated to be about 0.9 μm for the 0.8 NA objective used in our experiments, which roughly matches the thermal diffusion lengths of different media.

In a MIR-PHI setup, a MIR laser is used as a pump beam to create absorption events on sample objects. A temperature gradient is thereby created around the heated particles. Since the refractive index of the medium is directly related to its temperature, a temperature gradient will result in a refractive index gradient, which can then be detected by another probe beam. The information of the absorption event is therefore measured indirectly by the change in the probe light. Since the light being measured is the probe (usually visible light), it is no longer constrained by the Abbe diffraction limit of the MIR, and only detectors for visible light are needed.

The integration time on the lock-in of the lock-in amplifier120is an important factor in system performance as well. Generally, the longer the integration time, the better the signal-to-noise for the PHI signal. However, extended lock-in integration time substantially deteriorates MIR-PHI's real-time detection capability. In experiments described herein, 300 ms is used under CS2medium, and 1 second is used for glycerin and water.

FIGS.2A and2Bare images of polystyrene beads in water captured by the example photothermal imaging system using a 532 nm probe laser. In image200, the mid-IR pump beam is configured to operate at a wavelength of 2850 cm−1. In image250, the mid-IR pump beam is configured to operate at a wavelength of 3300 cm−1.

These two wavelengths were selected to form the largest contrast between two materials. At 3300 cm−1, water has a high absorption strength, while the absorption from polystyrene should be low. On the other hand, at 2850 cm−1, polystyrene has a local maxima in its mid-IR absorption spectra, while the absorption from water falls to around 20%. Hence, the two chosen wavelengths 3300 cm−1and 2850 cm−1should form complementary images with relatively high contrasts.

The results, as shown inFIGS.2A and2B, confirmed the above prediction. InFIG.2A, the polystyrene beads appear to have a negative photothermal signal (lower than the background). In contrast, inFIG.2B, the polystyrene beads appear positive (higher than the background). The S/N ratio measured at 3300 cm−1is 4, while at 2850 cm−1it becomes around 11.

Post-processing of the resulting images can be done to improve the S/N level. For example, image fusion can be a viable method to enhance image contrast. However, in this example, a specific algorithm could be used to properly combine the two images, in order to retain the appropriate chemical information.

FIG.3Ais a MIR-PHI image300of a polystyrene bead in glycerin captured by the example photothermal imaging system. As shown inFIG.3A, when a polystyrene bead is placed in glycerin, strong MIR-PHI signal was obtained (S/N>100) within a short acquisition time. By fitting the signal intensity profile into a Gaussian beam profile, a Gaussian beam waist of 0.62 μm±0.01 μm was measured, which is much smaller than the Abbe diffraction limit of the MIR light (around 2.33 μm). Thus, super-resolution was experimentally achieved.

FIG.3Bis a line-profile graph350of the MIR-PHI image ofFIG.3Aacross the polystyrene bead. The line-profile graph350may be used to calculate performance factors, such as signal-to-noise ratio (SNR). As shown inFIG.3B, the photothermal imaging system achieved an SNR of 106, with a Gaussian beam waist of approximately 0.62 μm, for a polystyrene bead in glycerin. This image demonstrates for example the ability MIR-PHI embodiments described in this disclosure to achieve spatially resolved measurements of IR absorption with sub-micron spatial resolution.

FIG.4is an image400of polystyrene beads in air on a glass coverslip captured by the example photothermal imaging system. InFIG.4, bare polystyrene beads were not immersed in any liquid media, but simply drop-cast on a glass coverslip. While maintaining at a high contrast, the shape of the MIR-PHI signal is not perfectly spherical, which may be due to a slight misalignment in the optical system. The MIR-PHI signal may be improved by implementing a movable piezo z-axis objective mount, and finer micrometer actuators for positioning.

FIG.5Ais a MIR-PHI spectrum graph500of a polystyrene bead on a glass coverslip captured by the example photothermal imaging system. When both the pump beam and the probe beam rest on one particle, tuning the pump beam (MIR) can provide local absorption spectral information. The MIR-PHI signal can be measured at a plurality of wavelengths of the pump beam, resulting in a clear MIR absorption spectrum of PS beads as shown inFIG.5A.

FIG.5Bis an ensemble Fourier transform infrared measurement (FT-IR)550the polystyrene bead on a glass coverslip fromFIG.5A. The MIR-PHI spectrum graph500ofFIG.5Aroughly matches with the ensemble FT-IR measurement of the polystyrene beads sample inFIG.5B. Slight differences in peak positions are due to the mismatch in scanning spectral step-size (4 cm−1vs 2 cm−1).

Although the peaks inFIG.5AandFIG.5Bare not precisely identical, the two graphs illustrate that a MIR-PHI spectrum can serve to identify the chemical composition of a sample. In this example, the MIR-PHI spectrum peaks inFIG.5Acorrespond with spectrum dips in the FT-IR spectrum inFIG.5B. Given a database of known FT-IR spectra, the MIR-PHI spectrum of some sample may be compared against that database to identify the chemical composition of that sample.

FIGS.6A-6Dare MIR-PHI measurements of a polystyrene bead in carbon disulfide (CS2) at different irradiance configurations, captured by the example photothermal imaging system. InFIG.6A, image600and line-profile graph605are MIR-PHI measurements taken with a radiance level of 0.1 Watts, resulting in a Gaussian beam waist of 0.85 μm and a signal-to-noise ratio (SNR) of 19. InFIG.6B, image610and line-profile graph615are MIR-PHI measurements taken with a radiance level of 0.35 Watts, resulting in a Gaussian beam waist of 0.94 μm and an SNR of 24. InFIG.6C, image620and line-profile graph625are MIR-PHI measurements taken with a radiance level of 0.5 Watts, resulting in a Gaussian beam waist of 1.02 μm and an SNR of 28. InFIG.6D, image630and line-profile graph635are MIR-PHI measurements taken with a radiance level of 0.6 Watts, resulting in a Gaussian beam waist of 1.03 μm and an SNR of 19.

FIGS.6A-6Dwere produced as a part of a probe irradiance dependence study, which was performed to investigate the optimal probe irradiance levels. An area of 15×15 μm is studied (scanning step size: 0.5 μm), with single 1.1 μm polystyrene beads present in the field of view.

Comparison betweenFIGS.6A,6B, and6Cclearly shows an enhancement of the signal level with an increase in the probe irradiance. However, comparing the results betweenFIG.6CandFIG.6Ddisplays an opposite trend, where a radiance level increase caused a decrease in signal level. Possible sample damage may explain the signal degradation betweenFIG.6CandFIG.6D. In this example, probe irradiance around 0.4 W was determined to be preferred for this system.

The line profiles in the graphs ofFIGS.6A-6Dwere used to estimate the spatial resolution. The results show that the beam waist is around 1 μm, very similar to the size of the polystyrene beads. On the other hand, the theoretical Abbe diffraction limit of the pump beam is

λ2*NA=2.33μm.
Thus, super-resolution MIR imaging was achieved.

Although some nonpolar media, such as CS2and CCl4have excellent photothermal properties and minimum infrared absorption, their low boiling points and poor biocompatibility substantially lessen their potential for applications. Bio-compatible media, such as glycerin and water, are of much greater interest. Hence, it is worthwhile to test the photothermal imaging system with the 1.1 μm polystyrene beads in both glycerin and water.

FIG.7Ais a MIR-PHI image700of a polystyrene bead in glycerin, captured by the example photothermal imaging system.FIG.7Bis a MIR-PHI line-profile graph750of the MIR-PHI image700shown inFIG.7A. Glycerin, although not as ideal as CS2and CCl4for a photothermal medium, is still around 5 times better than water for this application. InFIG.7, a single 1.1 μm polystyrene bead is imaged with much smaller step size (0.2 μm). However, the signal-to-noise ratio falls to the order of 11, which makes this measurement more difficult. The reason for the inferior S/N ratio lies in the fact that the mid-IR absorptions of Glycerin and polystyrene are largely overlapped in this region. Therefore the background signal level for the PHI image is much higher than that from CS2, which in turn gives rise to the deteriorated S/N ratio.

This disadvantage was believed to be even more severe for measurements in water, because of the broad absorption band that water has in the mid-IR region. In order to alleviate this issue, the absorption of the polystyrene and water were carefully compared. The tests and results of the MIR-PHI imaging of polystyrene beads in water is described above in relation toFIGS.2A and2B.

In summary, MIR-PHI not only significantly improves the spatial resolution of traditional MIR imaging, but also circumvents the usage of a sophisticated mid-IR detector. It therefore provides a robust super-resolution MIR imaging solution in an inexpensive way. The experimental results show that measurements in CS2imaged 1.1 μm polystyrene beads at a reasonable S/N ratio, with super-resolution capabilities. Results in glycerin and water, although displaying similar resolution, have much poorer S/N ratios. Hyperspectral imaging will be performed in the near future to exploit the wealth of chemical information in the mid-IR region. Also, fine tuning in the z axis of the imaging plane can be a direction to significantly improve the signal-to-noise ratio. Image post-processing with image fusion or other methods can potentially enhance the imaging contrast too.

Although certain example methods and apparatus have been described herein, the scope of coverage of this patent is not limited thereto. On the contrary, this patent covers all methods, apparatus, and articles of manufacture fairly falling within the scope of the appended claims either literally or under the doctrine of equivalents.

It should be understood that arrangements described herein are for purposes of example only. As such, those skilled in the art will appreciate that other arrangements and other elements (e.g., machines, interfaces, operations, orders, and groupings of operations, etc.) can be used instead, and some elements may be omitted altogether according to the desired results. Further, many of the elements that are described are functional entities that may be implemented as discrete or distributed components or in conjunction with other components, in any suitable combination and location, or other structural elements described as independent structures may be combined.

While various aspects and implementations have been disclosed herein, other aspects and implementations will be apparent to those skilled in the art. The various aspects and implementations disclosed herein are for purposes of illustration and are not intended to be limiting, with the true scope being indicated by the following claims, along with the full scope of equivalents to which such claims are entitled. It is also to be understood that the terminology used herein is for the purpose of describing particular implementations only, and is not intended to be limiting.