A microscope device comprising: a microscope objective , a first light source for transmitted light illumination of the sample with light within a first spectral range and a second light source for transmitted light illumination of the sample with light within a second spectral range different from the first spectral range, a tube lens for forming a sample-image from the light collected by the microscope objective, a first camera detector , and an analyzer unit , wherein the analyzer unit is configured to combine a first image of the sample and a second image of the sample so as to generate a total sample image with enhanced contrast, characterised in that : the microscope comprises a second camera detector , and a dichroic beam splitter in the imaging beam path between the tube lens and the camera detectors , wherein the dichroic beam splitter reflects light within the first spectral range onto the first camera detector and transmits light within the second spectral range onto the second camera detector , so that the first camera detector detects the first image of the sample from light within the first spectral range and the second camera detector detects the second image of the sample from light within the second spectral range, wherein the angle (a/2) of the surface normal of the dichroic beam splitter with regard to the incident beam lies between 12° and 18°. a microscope objective , a first light source for transmitted light illumination of the sample with light within a first spectral range and a second light source for transmitted light illumination of the sample with light within a second spectral range different from the first spectral range, a tube lens for forming a sample-image from the light collected by the microscope objective, a first camera detector , and an analyzer unit , wherein the analyzer unit is configured to combine a first image of the sample and a second image of the sample so as to generate a total sample image with enhanced contrast, characterised in that : the microscope comprises a second camera detector , and a dichroic beam splitter in the imaging beam path between the tube lens and the camera detectors , wherein the dichroic beam splitter reflects light within the first spectral range onto the first camera detector and transmits light within the second spectral range onto the second camera detector , so that the first camera detector detects the first image of the sample from light within the first spectral range and the second camera detector detects the second image of the sample from light within the second spectral range, wherein the angle (a/2) of the surface normal of the dichroic beam splitter with regard to the incident beam lies between 12° and 18°. the microscope comprises a second camera detector , and a dichroic beam splitter in the imaging beam path between the tube lens and the camera detectors , wherein the dichroic beam splitter reflects light within the first spectral range onto the first camera detector and transmits light within the second spectral range onto the second camera detector , so that the first camera detector detects the first image of the sample from light within the first spectral range and the second camera detector detects the second image of the sample from light within the second spectral range, wherein the angle (a/2) of the surface normal of the dichroic beam splitter with regard to the incident beam lies between 12° and 18°. <EOS>