An immunoassay cartridge comprising: a reaction chamber in which a plurality of antibodies or antigens are attached to an inner surface including a bottom surface closest to a fluorescence sensor assembly ; and a fluorescence sensor assembly disposed on a bottom surface of the reaction chamber , wherein the fluorescence sensor assembly comprises: an emission filter disposed below the reaction chamber , the emission filter having an optical characteristic capable of filtering an excitation light regardless of an incident angle of the excitation light and transmitting a radiation light having a wavelength larger than that of the excitation light; characterized in that the emission filter comprises: a base medium arranged in a flat shape and including a material which is transparent and which does not generate fluorescence or phosphorescence by excitation light; a semi-cured photoresist disposed in the base medium and fixed in a solid state by at least one method selected from the group consisting of thermosetting, photocuring and drying; and a pigment disposed in the base medium and absorbing light of a predetermined wavelength. a reaction chamber in which a plurality of antibodies or antigens are attached to an inner surface including a bottom surface closest to a fluorescence sensor assembly ; and a fluorescence sensor assembly disposed on a bottom surface of the reaction chamber , wherein the fluorescence sensor assembly comprises: an emission filter disposed below the reaction chamber , the emission filter having an optical characteristic capable of filtering an excitation light regardless of an incident angle of the excitation light and transmitting a radiation light having a wavelength larger than that of the excitation light; characterized in that the emission filter comprises: a base medium arranged in a flat shape and including a material which is transparent and which does not generate fluorescence or phosphorescence by excitation light; a semi-cured photoresist disposed in the base medium and fixed in a solid state by at least one method selected from the group consisting of thermosetting, photocuring and drying; and a pigment disposed in the base medium and absorbing light of a predetermined wavelength. an emission filter disposed below the reaction chamber , the emission filter having an optical characteristic capable of filtering an excitation light regardless of an incident angle of the excitation light and transmitting a radiation light having a wavelength larger than that of the excitation light; characterized in that the emission filter comprises: a base medium arranged in a flat shape and including a material which is transparent and which does not generate fluorescence or phosphorescence by excitation light; a semi-cured photoresist disposed in the base medium and fixed in a solid state by at least one method selected from the group consisting of thermosetting, photocuring and drying; and a pigment disposed in the base medium and absorbing light of a predetermined wavelength. a base medium arranged in a flat shape and including a material which is transparent and which does not generate fluorescence or phosphorescence by excitation light; a semi-cured photoresist disposed in the base medium and fixed in a solid state by at least one method selected from the group consisting of thermosetting, photocuring and drying; and a pigment disposed in the base medium and absorbing light of a predetermined wavelength. <EOS>