Method of detecting particles , comprising the steps of - providing a sample substance including particles to be detected on a source side of a separation path , - subjecting the sample substance to a separation force, so that the particles move along the separation path toward a downstream target side thereof, wherein the particles are separated from each other along the separation path and the particles have specific arrival times at the target side, wherein the arrival times depend on particle features and the separation force, and - detecting the particles in a detection volume , wherein - the separating step includes separating the particles of one particle type from particles of at least one other particle type and/or from remaining components of the sample substance by the effect of the separation force, wherein the arrival times are determined by different particle velocities along the separation path, characterized in that - the detecting step comprises an interferometric scattering detection of a series of single light scattering events created by the individual particles in the detection volume , wherein - the particles are bound to a detection surface of the detection volume or moved through the detection volume during the detecting step, and - scattering light signals obtained from the interferometric scattering detection are analysed for obtaining a particle mass or volume of the particles from a contrast of spots of scattering sources in the scattering light signals, and a particle size, charge, mobility measure, chemical affinity and/or concentration of the particles from arrival times of scattering sources in the scattering light signals. - providing a sample substance including particles to be detected on a source side of a separation path , - subjecting the sample substance to a separation force, so that the particles move along the separation path toward a downstream target side thereof, wherein the particles are separated from each other along the separation path and the particles have specific arrival times at the target side, wherein the arrival times depend on particle features and the separation force, and - detecting the particles in a detection volume , wherein - the separating step includes separating the particles of one particle type from particles of at least one other particle type and/or from remaining components of the sample substance by the effect of the separation force, wherein the arrival times are determined by different particle velocities along the separation path, - the detecting step comprises an interferometric scattering detection of a series of single light scattering events created by the individual particles in the detection volume , wherein - the particles are bound to a detection surface of the detection volume or moved through the detection volume during the detecting step, and - scattering light signals obtained from the interferometric scattering detection are analysed for obtaining a particle mass or volume of the particles from a contrast of spots of scattering sources in the scattering light signals, and a particle size, charge, mobility measure, chemical affinity and/or concentration of the particles from arrival times of scattering sources in the scattering light signals. <EOS>