A multiple unit enabled system for processing tissue in a closed pathway for maintaining sterility comprising: a centrifuge tube having an essentially cylindrical body having a top, an inverse conical apex and an interior volume and further comprising: at least two swabable valves ; an extraction tube releasably affixed to each of the at least two swabable valves that extends a distance within the interior volume towards the inverse conical apex ; a screen disposed within the interior volume of the body , the screen including a plurality of pores , the screen configured to support tissue introduced into the centrifuge tube ; and a filter being positioned below the screen in the inverse conical apex of the centrifuge tube , the filter including a plurality of pores, the plurality of pores for the filter being smaller than the plurality of pores for the screen ; and a container having at least one swabable valve, wherein the swabable valve can be releasably connected to one of the at least two swabable valves of the centrifuge tube for a sterile transfer of material contained with a closed system; wherein an end of the tubing is positioned in a section of the body between the screen and the filter ; wherein the plurality of pores of the screen are sized such that upon centrifugation, viscous tissue of the biological substance is broken down into smaller particles; and wherein the plurality of pores of the filter are sized such that upon centrifugation, the micronized tissue is squeezed through the screen and collected on top of a surface of the filter while a liquid fraction is moved downward below the filter . a centrifuge tube having an essentially cylindrical body having a top, an inverse conical apex and an interior volume and further comprising: at least two swabable valves ; an extraction tube releasably affixed to each of the at least two swabable valves that extends a distance within the interior volume towards the inverse conical apex ; a screen disposed within the interior volume of the body , the screen including a plurality of pores , the screen configured to support tissue introduced into the centrifuge tube ; and a filter being positioned below the screen in the inverse conical apex of the centrifuge tube , the filter including a plurality of pores, the plurality of pores for the filter being smaller than the plurality of pores for the screen ; and a container having at least one swabable valve, at least two swabable valves ; an extraction tube releasably affixed to each of the at least two swabable valves that extends a distance within the interior volume towards the inverse conical apex ; a screen disposed within the interior volume of the body , the screen including a plurality of pores , the screen configured to support tissue introduced into the centrifuge tube ; and a filter being positioned below the screen in the inverse conical apex of the centrifuge tube , the filter including a plurality of pores, the plurality of pores for the filter being smaller than the plurality of pores for the screen ; and a container having at least one swabable valve, wherein the swabable valve can be releasably connected to one of the at least two swabable valves of the centrifuge tube for a sterile transfer of material contained with a closed system; wherein an end of the tubing is positioned in a section of the body between the screen and the filter ; wherein the plurality of pores of the screen are sized such that upon centrifugation, viscous tissue of the biological substance is broken down into smaller particles; and wherein the plurality of pores of the filter are sized such that upon centrifugation, the micronized tissue is squeezed through the screen and collected on top of a surface of the filter while a liquid fraction is moved downward below the filter . <EOS>