A genotyping probe fluid, comprising: a liquid carrier; and a genotyping oligonucleotide in the liquid carrier, the genotyping oligonucleotide including: a first primer sequence; an index sequence portion directly attached to the first primer sequence; a priming site portion directly attached to the index sequence portion; a probe sequence directly attached to the priming site portion, the probe sequence being representative of a target genotyping locus having a nucleobase of interest, wherein a last base of the probe sequence is representative of a nucleobase of the target genotyping locus that is positioned directly adjacent to the nucleobase of interest; a restriction endonuclease site; and a second primer sequence that is at least partially complementary to a flow cell capture primer, wherein the restriction endonuclease site is positioned between the probe sequence and the second primer sequence, or the probe sequence is directly attached to the second primer sequence and the restriction endonuclease site is integrated into the second primer sequence at a cleavage distance from the last base of the probe sequence. a liquid carrier; and a genotyping oligonucleotide in the liquid carrier, the genotyping oligonucleotide including: a first primer sequence; an index sequence portion directly attached to the first primer sequence; a priming site portion directly attached to the index sequence portion; a probe sequence directly attached to the priming site portion, the probe sequence being representative of a target genotyping locus having a nucleobase of interest, wherein a last base of the probe sequence is representative of a nucleobase of the target genotyping locus that is positioned directly adjacent to the nucleobase of interest; a restriction endonuclease site; and a second primer sequence that is at least partially complementary to a flow cell capture primer, wherein the restriction endonuclease site is positioned between the probe sequence and the second primer sequence, or the probe sequence is directly attached to the second primer sequence and the restriction endonuclease site is integrated into the second primer sequence at a cleavage distance from the last base of the probe sequence. a first primer sequence; an index sequence portion directly attached to the first primer sequence; a priming site portion directly attached to the index sequence portion; a probe sequence directly attached to the priming site portion, the probe sequence being representative of a target genotyping locus having a nucleobase of interest, wherein a last base of the probe sequence is representative of a nucleobase of the target genotyping locus that is positioned directly adjacent to the nucleobase of interest; a restriction endonuclease site; and a second primer sequence that is at least partially complementary to a flow cell capture primer, wherein the restriction endonuclease site is positioned between the probe sequence and the second primer sequence, or the probe sequence is directly attached to the second primer sequence and the restriction endonuclease site is integrated into the second primer sequence at a cleavage distance from the last base of the probe sequence. <EOS>