A method of identifying maize event 4588.259, 4588.757 or 4588.652 in a sample comprising plant matter from the transgenic maize plant or part thereof, the method comprises: contacting a sample with a first primer and a second primer; amplifying a nucleic acid in the sample to obtain an amplified product; and detecting the amplified product specific to a target sequence in maize event 4588.259, 4588.757 or 4588.652, wherein the first primer comprises a nucleic acid sequence of SEQ ID NO: 38, the second primer comprises a nucleic acid of SEQ ID NO: 39 and the amplified product comprises a nucleic acid of SEQ ID NO: 51 specific to the target sequence of the event 4588.259; or wherein the first primer comprises a nucleic acid sequence of SEQ ID NO: 41, the second primer comprises a nucleic acid sequence of SEQ ID NO: 42 and the amplified product comprises a nucleic acid sequence of SEQ ID NO: 52 specific to the target sequence of the event 4588.652; or wherein the first primer comprises a nucleic acid sequence of SEQ ID NO: 47, the second primer comprises a nucleic acid sequence of SEQ ID NO: 45 and the amplified product comprises a nucleic acid sequence of SEQ ID NO: 54 specific to the target sequence of the event 4588.757; wherein each one of the maize events 4588.259, 4588.757 and 4588.652 is a transgenic maize plant or part thereof comprising one more synthetic nucleic acids encoding a glucanase and having a sequence with at least 90% identity to a reference sequence of SEQ ID NO: 8, wherein the maize event 4588.259 includes a target sequence comprising at least one polynucleotide of SEQ ID NOS: 22 - 26; the maize event 4588.757 includes a target sequence comprising at least one of polynucleotides of SEQ ID NOS: 27 and 28, and the maize event 4588.652 includes a target sequence comprising at least one polynucleotides of SEQ ID NO: 29 - 31, and wherein the detection of the amplified product indicates the presence of the maize event 4588.259, 4588.757 or 4588.652 in the sample. contacting a sample with a first primer and a second primer; amplifying a nucleic acid in the sample to obtain an amplified product; and detecting the amplified product specific to a target sequence in maize event 4588.259, 4588.757 or 4588.652, wherein the first primer comprises a nucleic acid sequence of SEQ ID NO: 38, the second primer comprises a nucleic acid of SEQ ID NO: 39 and the amplified product comprises a nucleic acid of SEQ ID NO: 51 specific to the target sequence of the event 4588.259; or wherein the first primer comprises a nucleic acid sequence of SEQ ID NO: 41, the second primer comprises a nucleic acid sequence of SEQ ID NO: 42 and the amplified product comprises a nucleic acid sequence of SEQ ID NO: 52 specific to the target sequence of the event 4588.652; or wherein the first primer comprises a nucleic acid sequence of SEQ ID NO: 47, the second primer comprises a nucleic acid sequence of SEQ ID NO: 45 and the amplified product comprises a nucleic acid sequence of SEQ ID NO: 54 specific to the target sequence of the event 4588.757; wherein each one of the maize events 4588.259, 4588.757 and 4588.652 is a transgenic maize plant or part thereof comprising one more synthetic nucleic acids encoding a glucanase and having a sequence with at least 90% identity to a reference sequence of SEQ ID NO: 8, wherein the maize event 4588.259 includes a target sequence comprising at least one polynucleotide of SEQ ID NOS: 22 - 26; the maize event 4588.757 includes a target sequence comprising at least one of polynucleotides of SEQ ID NOS: 27 and 28, and the maize event 4588.652 includes a target sequence comprising at least one polynucleotides of SEQ ID NO: 29 - 31, and wherein the detection of the amplified product indicates the presence of the maize event 4588.259, 4588.757 or 4588.652 in the sample. <EOS>