Arrangement for light sheet microscopy, comprising - a specimen plane (2.1) for arranging a specimen , - an illumination apparatus having a light source and an illumination optical unit, configured for producing a first light sheet (LB1) which extends in non-parallel fashion in relation to the specimen plane (2.1), for illuminating a first stripe of the specimen and for exciting fluorescence radiation in this first stripe of the specimen , and - a detection apparatus having a sensor for detecting the fluorescence radiation, having an imaging optical unit for imaging the fluorescence radiation from the first stripe of the specimen into a detection plane of the sensor , and having a detection axis which forms an angle with the first light sheet from an angle range of 70° to 110°, in particular having a detection axis that is perpendicular to the first light sheet, characterized - in that the illumination apparatus is configured to produce at least one further light sheet (LB2, LB3) that is arranged parallel to the first light sheet (LB1) but displaced in relation to the first light sheet (LB1) in the detection direction, i.e. along the detection axis , and in the illumination direction , for illuminating a further stripe of the specimen and for exciting fluorescence radiation in this further stripe of the specimen , and - in that the detection apparatus is configured to detect simultaneously the fluorescence radiation excited in the first stripe of the specimen by the first light sheet (LB1) and the fluorescence radiation excited in the further stripe of the specimen by the further light sheet (LB2, LB3). - a specimen plane (2.1) for arranging a specimen , - an illumination apparatus having a light source and an illumination optical unit, configured for producing a first light sheet (LB1) which extends in non-parallel fashion in relation to the specimen plane (2.1), for illuminating a first stripe of the specimen and for exciting fluorescence radiation in this first stripe of the specimen , and - a detection apparatus having a sensor for detecting the fluorescence radiation, having an imaging optical unit for imaging the fluorescence radiation from the first stripe of the specimen into a detection plane of the sensor , and having a detection axis which forms an angle with the first light sheet from an angle range of 70° to 110°, in particular having a detection axis that is perpendicular to the first light sheet, - in that the illumination apparatus is configured to produce at least one further light sheet (LB2, LB3) that is arranged parallel to the first light sheet (LB1) but displaced in relation to the first light sheet (LB1) in the detection direction, i.e. along the detection axis , and in the illumination direction , for illuminating a further stripe of the specimen and for exciting fluorescence radiation in this further stripe of the specimen , and - in that the detection apparatus is configured to detect simultaneously the fluorescence radiation excited in the first stripe of the specimen by the first light sheet (LB1) and the fluorescence radiation excited in the further stripe of the specimen by the further light sheet (LB2, LB3). <EOS>