A method for producing psicose, comprising a step of preparing an initial culture medium in which the ratio of the content (g/L) of carbon source to the content (g/L) of organic nitrogen source (C/N ratio) is 10/2 to 1/1, wherein the carbon source of the initial culture medium is at least one selected from the group consisting of psicose and fructose, and wherein the organic nitrogen source is at least one selected from the group consisting of yeast extract, corn steep liquor, soytone, peptone, soybean, soybean meal, cottonseed meal, molasses, casein, beef extract, malt extract and tryptone; a step of culturing a microorganism capable of producing psicose epimerase selected from the group consisting of Microbacterium foliorum, Microbacterium oxydans, Microbacterium phyllosphaerae and Ensifer adhaerens, in the initial culture medium which is performed in batch, in fed-batch or continuously; and a step of converting fructose into psicose, comprising reacting the cultured microorganism, a solution of the cultured microorganism, a lysate of the microorganism comprising psicose epimerase or a solution of the cultured microorganism, with fructose as a substrate, wherein the step of culturing is performed in a medium comprising 0.001 to 5 g/L of psicose as an inducer for increasing enzyme activity or expression of the microorganism under the condition that a pH variation value ΔpH of the medium is 0.05 to 0.5, wherein, in the step of culturing, an additional medium comprising psicose as a carbon source in which additional medium a C/N ratio is 20/1 to 1/1, is supplied intermittently or continuously so that the carbon source concentration of the medium is maintained at 0.001 g/L to 5 g/L, wherein an f-value defined as an increase in carbon source concentration (g/L) in a culture solution when a carbon source is added once in the additional medium is 0.2 to 0.5. a step of preparing an initial culture medium in which the ratio of the content (g/L) of carbon source to the content (g/L) of organic nitrogen source (C/N ratio) is 10/2 to 1/1, wherein the carbon source of the initial culture medium is at least one selected from the group consisting of psicose and fructose, and wherein the organic nitrogen source is at least one selected from the group consisting of yeast extract, corn steep liquor, soytone, peptone, soybean, soybean meal, cottonseed meal, molasses, casein, beef extract, malt extract and tryptone; a step of culturing a microorganism capable of producing psicose epimerase selected from the group consisting of Microbacterium foliorum, Microbacterium oxydans, Microbacterium phyllosphaerae and Ensifer adhaerens, in the initial culture medium which is performed in batch, in fed-batch or continuously; and a step of converting fructose into psicose, comprising reacting the cultured microorganism, a solution of the cultured microorganism, a lysate of the microorganism comprising psicose epimerase or a solution of the cultured microorganism, with fructose as a substrate, wherein the step of culturing is performed in a medium comprising 0.001 to 5 g/L of psicose as an inducer for increasing enzyme activity or expression of the microorganism under the condition that a pH variation value ΔpH of the medium is 0.05 to 0.5, wherein, in the step of culturing, an additional medium comprising psicose as a carbon source in which additional medium a C/N ratio is 20/1 to 1/1, is supplied intermittently or continuously so that the carbon source concentration of the medium is maintained at 0.001 g/L to 5 g/L, wherein an f-value defined as an increase in carbon source concentration (g/L) in a culture solution when a carbon source is added once in the additional medium is 0.2 to 0.5. <EOS>