A method for amplification in a microfluidic assay for analyte detection, comprising binding an analyte to a transport element, wherein the transport element comprises a particle and a first antibody element and the analyte is bound to the first antibody element ; moving the transport element with the analyte bound thereto towards a tracer storage site comprising tracer elements, wherein a tracer element comprises a first binder element , a label element and a second antibody element , wherein the tracer elements are attached to the tracer storage site using attachment elements having a low affinity that allows detachment of the tracer elements from the tracer storage site ; binding the analyte to the second antibody element ; moving the transport element with the analyte and the tracer element bound therewith towards a tracer transport site comprising second binder elements on the surface thereof, wherein the second binder elements are attached to the tracer transport site ; binding the first binder element with a second binder element ; moving the transport element with the analyte bound thereto towards the tracer storage site so that the analyte is detached from the second antibody element of the tracer element while the first binder element remains bound to the second binder element ; and repeating the steps subsequent to binding the analyte to the transport element a predetermined number of times; wherein the first antibody element has a higher affinity for the analyte than the second antibody element ; the second antibody element has a higher affinity for the analyte than for the tracer storage site ; and the affinity of the second antibody element towards the analyte is smaller than that between the first and second binder elements and that between the second antibody element and the first binder element . binding an analyte to a transport element, wherein the transport element comprises a particle and a first antibody element and the analyte is bound to the first antibody element ; moving the transport element with the analyte bound thereto towards a tracer storage site comprising tracer elements, wherein a tracer element comprises a first binder element , a label element and a second antibody element , wherein the tracer elements are attached to the tracer storage site using attachment elements having a low affinity that allows detachment of the tracer elements from the tracer storage site ; binding the analyte to the second antibody element ; moving the transport element with the analyte and the tracer element bound therewith towards a tracer transport site comprising second binder elements on the surface thereof, wherein the second binder elements are attached to the tracer transport site ; binding the first binder element with a second binder element ; moving the transport element with the analyte bound thereto towards the tracer storage site so that the analyte is detached from the second antibody element of the tracer element while the first binder element remains bound to the second binder element ; and repeating the steps subsequent to binding the analyte to the transport element a predetermined number of times; wherein the first antibody element has a higher affinity for the analyte than the second antibody element ; the second antibody element has a higher affinity for the analyte than for the tracer storage site ; and the affinity of the second antibody element towards the analyte is smaller than that between the first and second binder elements and that between the second antibody element and the first binder element . <EOS>