A process for enhanced recovery of a concentrated biological fluid from a crossflow filtration system , said crossflow filtration system comprising a biofluid container , a crossflow filtration filter , a feed flow line operably linked from said biofluid container to said crossflow filtration filter , a retentate flow line operably linked from said crossflow filtration filter to said biofluid container , a permeate flow line operably linked to said crossflow filtration filter , a recirculating pump in said feed flow line , a drain line operably linked to said feed flow line via a drain valve wherein said drain line is located between said recirculating pump and said crossflow filtration filter , a sterile air source , an internal pump operably linked to said retentate flow line and said sterile air source , wherein said internal pump is a positive displacement pump, such as a peristaltic pump, said process, after concentrating the biofluid in the biofluid source container by cycling the biofluid through the crossflow filtration system and collecting waste from the permeate line , comprising: a) discontinuing the pressurized biofluid flow from the recirculating pump , b) closing valves from and to the biofluid source container , c) opening said drain valve , d) inducing an internalized pressurized countercurrent air flow throughout the crossflow filtration system by activating the internal pump , and e) recovering the concentrated biological fluid at the drain valve . a biofluid container , a crossflow filtration filter , a feed flow line operably linked from said biofluid container to said crossflow filtration filter , a retentate flow line operably linked from said crossflow filtration filter to said biofluid container , a permeate flow line operably linked to said crossflow filtration filter , a recirculating pump in said feed flow line , a drain line operably linked to said feed flow line via a drain valve wherein said drain line is located between said recirculating pump and said crossflow filtration filter , a sterile air source , an internal pump operably linked to said retentate flow line and said sterile air source , wherein said internal pump is a positive displacement pump, such as a peristaltic pump, said process, after concentrating the biofluid in the biofluid source container by cycling the biofluid through the crossflow filtration system and collecting waste from the permeate line , comprising: a) discontinuing the pressurized biofluid flow from the recirculating pump , b) closing valves from and to the biofluid source container , c) opening said drain valve , d) inducing an internalized pressurized countercurrent air flow throughout the crossflow filtration system by activating the internal pump , and e) recovering the concentrated biological fluid at the drain valve . a) discontinuing the pressurized biofluid flow from the recirculating pump , b) closing valves from and to the biofluid source container , c) opening said drain valve , d) inducing an internalized pressurized countercurrent air flow throughout the crossflow filtration system by activating the internal pump , and e) recovering the concentrated biological fluid at the drain valve . <EOS>