A method for making a soybean plant comprising a mutation in: one or more FAD2-1A alleles, one or more FAD2-1B alleles, and one or more FAD3A alleles; the method comprising: (a) providing soybean plant parts or plant cells, wherein the plant parts or plant cells comprise a mutation in one or more FAD2-1A alleles and a mutation in one or more FAD2-1B alleles, wherein each FAD2-1A allele has a sequence as set forth in SEQ ID NO: 100, or has a sequence with at least 95% identity to SEQ ID NO: 100; wherein each FAD2-1B allele has a sequence as set forth in SEQ ID NO:101, or has a sequence with at least 95% identity to SEQ ID NO:101; and wherein the plant parts or plant cells comprise at least a functional FAD3A allele, (b) contacting the plant parts or plant cells with one or more TAL effector endonucleases targeted to an endogenous FAD3A sequence, wherein each of the TAL effector endonucleases is targeted to a pair of sequences as set forth in SEQ ID NOS 8 and 9, (c) regenerating the plant parts or plant cells into whole soybean plants, and (d) selecting from the whole soybean plants a soybean plant comprising a mutation in one or more FAD3A alleles, wherein each mutated FAD3A allele comprises a deletion of the cytosine nucleotide at position 515 of SEQ ID NO:24. one or more FAD2-1A alleles, one or more FAD2-1B alleles, and one or more FAD3A alleles; the method comprising: (a) providing soybean plant parts or plant cells, wherein the plant parts or plant cells comprise a mutation in one or more FAD2-1A alleles and a mutation in one or more FAD2-1B alleles, wherein each FAD2-1A allele has a sequence as set forth in SEQ ID NO: 100, or has a sequence with at least 95% identity to SEQ ID NO: 100; wherein each FAD2-1B allele has a sequence as set forth in SEQ ID NO:101, or has a sequence with at least 95% identity to SEQ ID NO:101; and wherein the plant parts or plant cells comprise at least a functional FAD3A allele, (b) contacting the plant parts or plant cells with one or more TAL effector endonucleases targeted to an endogenous FAD3A sequence, wherein each of the TAL effector endonucleases is targeted to a pair of sequences as set forth in SEQ ID NOS 8 and 9, (c) regenerating the plant parts or plant cells into whole soybean plants, and (d) selecting from the whole soybean plants a soybean plant comprising a mutation in one or more FAD3A alleles, (a) providing soybean plant parts or plant cells, wherein the plant parts or plant cells comprise a mutation in one or more FAD2-1A alleles and a mutation in one or more FAD2-1B alleles, wherein each FAD2-1A allele has a sequence as set forth in SEQ ID NO: 100, or has a sequence with at least 95% identity to SEQ ID NO: 100; wherein each FAD2-1B allele has a sequence as set forth in SEQ ID NO:101, or has a sequence with at least 95% identity to SEQ ID NO:101; and wherein the plant parts or plant cells comprise at least a functional FAD3A allele, wherein each FAD2-1A allele has a sequence as set forth in SEQ ID NO: 100, or has a sequence with at least 95% identity to SEQ ID NO: 100; wherein each FAD2-1B allele has a sequence as set forth in SEQ ID NO:101, or has a sequence with at least 95% identity to SEQ ID NO:101; and wherein the plant parts or plant cells comprise at least a functional FAD3A allele, (b) contacting the plant parts or plant cells with one or more TAL effector endonucleases targeted to an endogenous FAD3A sequence, wherein each of the TAL effector endonucleases is targeted to a pair of sequences as set forth in SEQ ID NOS 8 and 9, (c) regenerating the plant parts or plant cells into whole soybean plants, and (d) selecting from the whole soybean plants a soybean plant comprising a mutation in one or more FAD3A alleles, wherein each mutated FAD3A allele comprises a deletion of the cytosine nucleotide at position 515 of SEQ ID NO:24. <EOS>