Removable cryotransfer system for an electron microscope comprising at least one cartridge comprising at least one recess for holding a sample carrier optionally comprising a flexible element , at least one slit providing with respect to the cartridge parallel loading of the sample carrier, the sample carrier being provided on a support, and a fixing element , preferably a preloaded fixing element , a cartridge block (CB) for incorporating the at least one cartridge, a cartridge block transporter (CBT) comprising a cartridge block mover , and a cartridge block connector , and preferably at least one of an O-ring , a cartridge mover , a pushing aid , a fastener , and a vacuum coupler , a cryo vacuum transfer chamber comprising a tube adapted to receive a microscope sample holder , a valve arranged to receive the cartridge block transporter , and an aligner provided inside tube and arranged for receiving the cartridge block thereon in order to position the cartridge into the tip of a microscope sample holder , a cooling element connected to the aligner in operation in thermal contact with a cooling liquid to keep the aligner at a temperature of < 100K, and optionally at least one of a viewing window , a vacuum pump connector , a further valve , wherein the cooling element preferably comprises a cooling rod , a tube , a vacuum tight connector , and a metal braid , and the microscope sample holder with a tip , a cooling rod , a cooling element in operation adapted to maintain the sample in thermal contact with the cooling rod and the tip to keep the sample at a temperature of < 100K, and optionally at least one of an O-ring , a connector , a foldable protection , a counter grip , a dock , and a passage . at least one cartridge comprising at least one recess for holding a sample carrier optionally comprising a flexible element , at least one slit providing with respect to the cartridge parallel loading of the sample carrier, the sample carrier being provided on a support, and a fixing element , preferably a preloaded fixing element , a cartridge block (CB) for incorporating the at least one cartridge, a cartridge block transporter (CBT) comprising a cartridge block mover , and a cartridge block connector , and preferably at least one of an O-ring , a cartridge mover , a pushing aid , a fastener , and a vacuum coupler , a cryo vacuum transfer chamber comprising a tube adapted to receive a microscope sample holder , a valve arranged to receive the cartridge block transporter , and an aligner provided inside tube and arranged for receiving the cartridge block thereon in order to position the cartridge into the tip of a microscope sample holder , a cooling element connected to the aligner in operation in thermal contact with a cooling liquid to keep the aligner at a temperature of < 100K, and optionally at least one of a viewing window , a vacuum pump connector , a further valve , wherein the cooling element preferably comprises a cooling rod , a tube , a vacuum tight connector , and a metal braid , and the microscope sample holder with a tip , a cooling rod , a cooling element in operation adapted to maintain the sample in thermal contact with the cooling rod and the tip to keep the sample at a temperature of < 100K, and optionally at least one of an O-ring , a connector , a foldable protection , a counter grip , a dock , and a passage . <EOS>