A method of controlling a process in a closed cell expansion system, the method comprising: providing a fluid conveyance assembly , wherein the fluid conveyance assembly comprises a bioreactor ; engaging the fluid conveyance assembly; providing an air removal chamber , wherein the air removal chamber is mounted on the fluid conveyance assembly , and wherein the air removal chamber comprises a fluid containment chamber , the fluid containment chamber comprising: a fluid entrance aperture ; a fluid exit aperture , wherein the fluid exit aperture is coupled to a fluid exit tube ; and a vent aperture , wherein the vent aperture is located above the fluid entrance aperture and the fluid exit aperture; providing a sensor to detect the fluid level in the fluid containment chamber ; providing a pump to pump a fluid through the fluid entrance aperture and into the fluid containment chamber of the air removal chamber ; initiating the process, wherein the process comprises loading reagent from a reagent bag into the bioreactor until the reagent bag is empty, wherein the reagent is the fluid, comprising: operating the pump to pump the fluid through the fluid entrance aperture and into the fluid containment chamber, wherein the fluid reaches a fluid level in the fluid containment chamber; allowing the fluid to pass through the fluid exit aperture to enter the fluid exit tube ; detecting the fluid level using the sensor ; providing a stop condition, comprising: meeting the stop condition when the fluid level in the fluid containment chamber reaches a predetermined level as detected by the sensor ; and when the fluid level in the fluid containment chamber is at the predetermined level, stopping the process based on meeting the stop condition. providing a fluid conveyance assembly , wherein the fluid conveyance assembly comprises a bioreactor ; engaging the fluid conveyance assembly; providing an air removal chamber , wherein the air removal chamber is mounted on the fluid conveyance assembly , and wherein the air removal chamber comprises a fluid containment chamber , the fluid containment chamber comprising: a fluid entrance aperture ; a fluid exit aperture , wherein the fluid exit aperture is coupled to a fluid exit tube ; and a vent aperture , wherein the vent aperture is located above the fluid entrance aperture and the fluid exit aperture; a fluid entrance aperture ; a fluid exit aperture , wherein the fluid exit aperture is coupled to a fluid exit tube ; and a vent aperture , wherein the vent aperture is located above the fluid entrance aperture and the fluid exit aperture; providing a sensor to detect the fluid level in the fluid containment chamber ; providing a pump to pump a fluid through the fluid entrance aperture and into the fluid containment chamber of the air removal chamber ; initiating the process, wherein the process comprises loading reagent from a reagent bag into the bioreactor until the reagent bag is empty, wherein the reagent is the fluid, comprising: operating the pump to pump the fluid through the fluid entrance aperture and into the fluid containment chamber, wherein the fluid reaches a fluid level in the fluid containment chamber; allowing the fluid to pass through the fluid exit aperture to enter the fluid exit tube ; detecting the fluid level using the sensor ; operating the pump to pump the fluid through the fluid entrance aperture and into the fluid containment chamber, wherein the fluid reaches a fluid level in the fluid containment chamber; allowing the fluid to pass through the fluid exit aperture to enter the fluid exit tube ; detecting the fluid level using the sensor ; providing a stop condition, comprising: meeting the stop condition when the fluid level in the fluid containment chamber reaches a predetermined level as detected by the sensor ; and when the fluid level in the fluid containment chamber is at the predetermined level, stopping the process based on meeting the stop condition. <EOS>