A device for lysing plasmid-containing bacterial cells in a controlled manner so as to extract cellular components of interest, comprising: a first tank and second tank in fluid communication with a high shear, low-residence time mixing device, wherein the first tank is configured to hold a suspension of cells containing cellular components of interest, the second tank is configured to hold a lysis solution, and the suspension of cells from the first tank and the lysis solution from the second tank are both allowed to flow into the mixing device forming a first fluid, wherein the high shear, low residence-time mixing device has a residence time equal to or less than about 1 second and the device is configured to flow the first fluid from the mixing device into a holding coil, and a bubble-mixer chamber in fluid communication with the holding coil; a first inlet configured to introduce the first fluid into the bubble-mixer chamber; one or more additional inlets configured to introduce an additional fluid into the bubble-mixer chamber; a means for introducing gas bubbles into the bubble-mixer chamber at a rate sufficient to cause substantial mixing of the first fluid with the additional fluid; a vent configured to allow excess gas to escape from the bubble-mixer chamber; and an outlet for the first fluid and the additional fluid after becoming substantially mixed; wherein the residence time of the holding coil is 2 to 10 minutes; wherein the means configured to introduce gas bubbles into the mixing chamber is a third inlet port; wherein the cellular component of interest is plasmid DNA; and the additional fluid comprises a neutralization solution, a precipitation solution, or a combination thereof. a first tank and second tank in fluid communication with a high shear, low-residence time mixing device, wherein the first tank is configured to hold a suspension of cells containing cellular components of interest, the second tank is configured to hold a lysis solution, and the suspension of cells from the first tank and the lysis solution from the second tank are both allowed to flow into the mixing device forming a first fluid, wherein the high shear, low residence-time mixing device has a residence time equal to or less than about 1 second and the device is configured to flow the first fluid from the mixing device into a holding coil, and a bubble-mixer chamber in fluid communication with the holding coil; a first inlet configured to introduce the first fluid into the bubble-mixer chamber; one or more additional inlets configured to introduce an additional fluid into the bubble-mixer chamber; a means for introducing gas bubbles into the bubble-mixer chamber at a rate sufficient to cause substantial mixing of the first fluid with the additional fluid; a vent configured to allow excess gas to escape from the bubble-mixer chamber; and an outlet for the first fluid and the additional fluid after becoming substantially mixed; wherein the residence time of the holding coil is 2 to 10 minutes; wherein the means configured to introduce gas bubbles into the mixing chamber is a third inlet port; wherein the cellular component of interest is plasmid DNA; and wherein the residence time of the holding coil is 2 to 10 minutes; wherein the means configured to introduce gas bubbles into the mixing chamber is a third inlet port; wherein the cellular component of interest is plasmid DNA; and the additional fluid comprises a neutralization solution, a precipitation solution, or a combination thereof. <EOS>