A method for the large-scale high-content analysis of biological samples, comprising the following steps a) Providing a reversed open microwell system which includes an array of open microwells , at least one reservoir for reagents and at least one reservoir for one or more biological samples, at least one microchannel , at least one input port for reagents and/or for one or more biological samples and at least one output port for them, said input and output ports being in microfluidic communication with one or more of said microchannels , wherein said microchannel has a cross-section area of micrometric dimensions and provides fluid to said microwells , wherein some of said reservoirs are precharged with said reagents; b) Providing an automated management system of said reversed open microwell system which comprises the following features: incubator at controlled temperature, humidity and CO 2 , fluid dispensing system comprising a tip , phase-contrast and fluorescence image acquisition; c) Placing said reversed open microwell system in said automated system; d) Charging reagents through one or more of said input ports , wherein said reagents comprise: filling buffer and/or washing solution and/or one or more drugs and/or one or more dyes, and/or one or more labeled antibodies and or one or more cell viability markers; e) Charging said one or more biological samples through one or more of said input ports ; wherein said charging of reagents and of the at least one biological sample is done by means of said fluid dispensing systems, said tip taking said reagents and said at least one biological sample from the reservoirs and charging into the input ports ; i) Optionally, dyeing said cells with one or more dyes and/or one or more labeled antibodies and or one or more cell viability markers; j) Acquiring images from one or more of said microwells , wherein said images are defined images TO; p) Classifying the cells displayed, wherein said classification is made with morphological and/or functional parameters detected from the images TO. a) Providing a reversed open microwell system which includes an array of open microwells , at least one reservoir for reagents and at least one reservoir for one or more biological samples, at least one microchannel , at least one input port for reagents and/or for one or more biological samples and at least one output port for them, said input and output ports being in microfluidic communication with one or more of said microchannels , wherein said microchannel has a cross-section area of micrometric dimensions and provides fluid to said microwells , wherein some of said reservoirs are precharged with said reagents; b) Providing an automated management system of said reversed open microwell system which comprises the following features: incubator at controlled temperature, humidity and CO 2 , fluid dispensing system comprising a tip , phase-contrast and fluorescence image acquisition; c) Placing said reversed open microwell system in said automated system; d) Charging reagents through one or more of said input ports , wherein said reagents comprise: filling buffer and/or washing solution and/or one or more drugs and/or one or more dyes, and/or one or more labeled antibodies and or one or more cell viability markers; e) Charging said one or more biological samples through one or more of said input ports ; i) Optionally, dyeing said cells with one or more dyes and/or one or more labeled antibodies and or one or more cell viability markers; j) Acquiring images from one or more of said microwells , wherein said images are defined images TO; p) Classifying the cells displayed, wherein said classification is made with morphological and/or functional parameters detected from the images TO. <EOS>