A micro-fluidic chip, comprising a chip main body, a sample inlet , an air inlet , a liquid driving force inlet , an air subchannel , a main fluid channel and multiple functional chambers provided on the chip main body; the main fluid channel communicates with the multiple functional chambers, the liquid driving force inlet connects to a liquid driving device to facilitate liquid flow in the main fluid channel and the multiple functional chambers; at least one of the multiple functional chambers is a liquid quantification chamber; the liquid quantification chamber has a predetermined volume, and a liquid identification site is provided at a liquid outlet of the liquid quantification chamber, and liquid to be quantified flows into the liquid quantification chamber from a liquid inlet of the liquid quantification chamber and reaches the liquid outlet after filling the liquid quantification chamber; wherein a surface of the liquid quantification chamber is formed by hydrophilic surface modification; a width of the liquid inlet of the liquid quantification chamber is 0.3-5 mm, and a height of the liquid inlet of the liquid quantification chamber is 0.3-3 mm; the liquid outlet of the liquid quantification chamber has a width of 0.3-5 mm and a height of 0.3-3 mm; at least one of the liquid quantification chamber is a sample quantification chamber , and a liquid sample flows into the sample quantification chamber through the sample inlet for quantification; one end of the air subchannel communicates with the air inlet , and the other end communicates with the main fluid channel between the sample quantification chamber and the sample inlet , and a junction point of the other end of the air subchannel and the main fluid channel is 0.5-10 mm away from a liquid inlet of the sample quantification chamber . the main fluid channel communicates with the multiple functional chambers, the liquid driving force inlet connects to a liquid driving device to facilitate liquid flow in the main fluid channel and the multiple functional chambers; at least one of the multiple functional chambers is a liquid quantification chamber; the liquid quantification chamber has a predetermined volume, and a liquid identification site is provided at a liquid outlet of the liquid quantification chamber, and liquid to be quantified flows into the liquid quantification chamber from a liquid inlet of the liquid quantification chamber and reaches the liquid outlet after filling the liquid quantification chamber; wherein a surface of the liquid quantification chamber is formed by hydrophilic surface modification; a width of the liquid inlet of the liquid quantification chamber is 0.3-5 mm, and a height of the liquid inlet of the liquid quantification chamber is 0.3-3 mm; the liquid outlet of the liquid quantification chamber has a width of 0.3-5 mm and a height of 0.3-3 mm; at least one of the liquid quantification chamber is a sample quantification chamber , and a liquid sample flows into the sample quantification chamber through the sample inlet for quantification; one end of the air subchannel communicates with the air inlet , and the other end communicates with the main fluid channel between the sample quantification chamber and the sample inlet , and a junction point of the other end of the air subchannel and the main fluid channel is 0.5-10 mm away from a liquid inlet of the sample quantification chamber . <EOS>