A light sheet microscope , comprising: an object slide configured to hold an object on a slide plane , an optical illumination system including an illumination objective configured to illuminate a first plane of the object with a light sheet, said first object plane being oblique relative to the slide plane , and an optical detection system including a detection objective and an image sensor device configured to define a first image plane which is orthogonal to an optical axis (OD) of the detection objective , wherein the detection objective is configured to image a focal plane onto the first image plane , said focal plane being coincident with the first object plane illuminated with the light sheet, wherein the image sensor device is further configured to define a second image plane which is tilted relative to the first image plane , and the detection objective is configured to image a second plane of the object onto the second image plane , said second object plane being parallel to or coincident with the slide plane , and wherein the image sensor device comprises a first image sensor defining the first image plane and a second image sensor defining the second image plane , or wherein the image sensor device comprises one single image sensor which is tiltable between a first position in which a light receiving layer of the single image sensor is oriented to define the first image plane , and a second position, in which the light receiving layer is oriented to define the second image plane , characterized in that the optical detection system comprises an aperture configured to define a light bundle (LB) for imaging the second object plane onto the second image plane ; and the aperture is located eccentrically with respect to the optical axis (D) of the detection objective . an object slide configured to hold an object on a slide plane , an optical illumination system including an illumination objective configured to illuminate a first plane of the object with a light sheet, said first object plane being oblique relative to the slide plane , and an optical detection system including a detection objective and an image sensor device configured to define a first image plane which is orthogonal to an optical axis (OD) of the detection objective , wherein the detection objective is configured to image a focal plane onto the first image plane , said focal plane being coincident with the first object plane illuminated with the light sheet, wherein the image sensor device is further configured to define a second image plane which is tilted relative to the first image plane , and the detection objective is configured to image a second plane of the object onto the second image plane , said second object plane being parallel to or coincident with the slide plane , and wherein the image sensor device comprises a first image sensor defining the first image plane and a second image sensor defining the second image plane , or wherein the image sensor device comprises one single image sensor which is tiltable between a first position in which a light receiving layer of the single image sensor is oriented to define the first image plane , and a second position, in which the light receiving layer is oriented to define the second image plane , characterized in that the optical detection system comprises an aperture configured to define a light bundle (LB) for imaging the second object plane onto the second image plane ; and the aperture is located eccentrically with respect to the optical axis (D) of the detection objective . <EOS>