A computerized system for designing and synthesizing nucleic acid sequences for gene expression comprising; a. a server for hosting a database , wherein the database comprises a plurality of entries, each entry comprising a nucleic acid sequence encoding for a harmful gene expression product, wherein the nucleic acid sequence encoding for the harmful gene expression product is from a bacterial genome or a viral genome; b. a network connection c. a computer readable medium , wherein said computerized system is configured for operating in a method of: i.) visualizing biological information in an interactive user interface relating to design instructions; ii.) designing nucleic acid sequences; iii.) automatically flagging when the nucleic acid sequence encoding for the harmful gene expression product is included in the nucleic acid sequence for at least one gene; iv.) automatically modifying the interactive user interface to notify when the nucleic acid sequence encoding for the harmful gene expression product is detected in the nucleic acid sequence for at least one gene; v.) providing an alternative alteration of the designed nucleic acid sequences, wherein the alternative alteration does not encode for the nucleic acid sequence encoding for the harmful gene expression product; and vi.) synthesizing a nucleic acid encoding for the alternative alteration of the designed nucleic acid sequences. a. a server for hosting a database , wherein the database comprises a plurality of entries, each entry comprising a nucleic acid sequence encoding for a harmful gene expression product, wherein the nucleic acid sequence encoding for the harmful gene expression product is from a bacterial genome or a viral genome; b. a network connection c. a computer readable medium , wherein said computerized system is configured for operating in a method of: i.) visualizing biological information in an interactive user interface relating to design instructions; ii.) designing nucleic acid sequences; iii.) automatically flagging when the nucleic acid sequence encoding for the harmful gene expression product is included in the nucleic acid sequence for at least one gene; iv.) automatically modifying the interactive user interface to notify when the nucleic acid sequence encoding for the harmful gene expression product is detected in the nucleic acid sequence for at least one gene; v.) providing an alternative alteration of the designed nucleic acid sequences, wherein the alternative alteration does not encode for the nucleic acid sequence encoding for the harmful gene expression product; and vi.) synthesizing a nucleic acid encoding for the alternative alteration of the designed nucleic acid sequences. i.) visualizing biological information in an interactive user interface relating to design instructions; ii.) designing nucleic acid sequences; iii.) automatically flagging when the nucleic acid sequence encoding for the harmful gene expression product is included in the nucleic acid sequence for at least one gene; iv.) automatically modifying the interactive user interface to notify when the nucleic acid sequence encoding for the harmful gene expression product is detected in the nucleic acid sequence for at least one gene; v.) providing an alternative alteration of the designed nucleic acid sequences, wherein the alternative alteration does not encode for the nucleic acid sequence encoding for the harmful gene expression product; and vi.) synthesizing a nucleic acid encoding for the alternative alteration of the designed nucleic acid sequences. <EOS>