A digital holographic microscope, DHM, comprising - A coherent light source for creating a base light beam for illuminating a sample in a first image plane , - A sample holder for holding a sample in the first image plane to be illuminated, - A detector, e.g. a camera , arranged to record images of light transmitted through a sample in the sample holder, - A first beam splitter for dividing the base light beam from the coherent light source into at least a first divided beam and a second divided beam, said first beam splitter for dividing the base light beam into different portions being located upstream or downstream of the sample holder, and - A light beam guiding system for guiding said base light beam or said first divided light beam through the sample and further arranged to guide said first and second divided beam to reunite at a reuniting point before the first and second beams are directed to the detector, wherein said digital holographic microscope further comprises a light reducing arrangement for reducing the intensity of the light in the first divided beam, said light reducing arrangement comprising - at least a first lens for collimating the light in the first divided beam scattered by a particle comprised in the sample and focusing the unscattered light passing through the sample in a focal plane , and - a partially light transparent spatial filter arranged at or in the vicinity of the focal plane of said first lens in order to reduce the intensity of the focused unscattered light passing through the sample located in the first image plane such that the majority, but not all, of unscattered light passing through the sample is filtered off and the majority of the light scattered by a particle in the sample is guided via a light guiding system to reunite with said second divided beam. - A coherent light source for creating a base light beam for illuminating a sample in a first image plane , - A sample holder for holding a sample in the first image plane to be illuminated, - A detector, e.g. a camera , arranged to record images of light transmitted through a sample in the sample holder, - A first beam splitter for dividing the base light beam from the coherent light source into at least a first divided beam and a second divided beam, said first beam splitter for dividing the base light beam into different portions being located upstream or downstream of the sample holder, and - A light beam guiding system for guiding said base light beam or said first divided light beam through the sample and further arranged to guide said first and second divided beam to reunite at a reuniting point before the first and second beams are directed to the detector, wherein said digital holographic microscope further comprises a light reducing arrangement for reducing the intensity of the light in the first divided beam, said light reducing arrangement comprising - at least a first lens for collimating the light in the first divided beam scattered by a particle comprised in the sample and focusing the unscattered light passing through the sample in a focal plane , and - a partially light transparent spatial filter arranged at or in the vicinity of the focal plane of said first lens in order to reduce the intensity of the focused unscattered light passing through the sample located in the first image plane such that the majority, but not all, of unscattered light passing through the sample is filtered off and the majority of the light scattered by a particle in the sample is guided via a light guiding system to reunite with said second divided beam. <EOS>