Microfluidic cell culture chip that contains a central module comprising: - a central unit , which contains -- a support consisting of a non-absorbable membrane , including an upper face and a lower face , perforated by at least one perforation , said support being composed in particular of bioprinted plastic, polycarbonate, tissue culture plastic, glass or SU8, -- a 3D nanostructured porous membrane , including an upper face and a lower face , and including at least one protrusion , said at least one protrusion being hollow and including an outer face and an inner face and forming a structure in relief on the upper face side of the 3D nanostructured membrane , said protrusion being in particular in the shape of a hollow dome having a circular base , oo said lower face of said 3D nanostructured porous membrane being positioned in a rigidly connected manner on said upper face of said support , oo or said upper face of the 3D nanostructured membrane being positioned in a rigidly connected manner on said lower face of said support , oo and said 3D nanostructured porous membrane and the at least one protrusion being composed of materials suitable for the culture of two types of distinct cells; - a base , said central unit being integrated into said base , and forming a whole with said base . - a central unit , which contains -- a support consisting of a non-absorbable membrane , including an upper face and a lower face , perforated by at least one perforation , said support being composed in particular of bioprinted plastic, polycarbonate, tissue culture plastic, glass or SU8, -- a 3D nanostructured porous membrane , including an upper face and a lower face , and including at least one protrusion , said at least one protrusion being hollow and including an outer face and an inner face and forming a structure in relief on the upper face side of the 3D nanostructured membrane , said protrusion being in particular in the shape of a hollow dome having a circular base , oo said lower face of said 3D nanostructured porous membrane being positioned in a rigidly connected manner on said upper face of said support , oo or said upper face of the 3D nanostructured membrane being positioned in a rigidly connected manner on said lower face of said support , oo and said 3D nanostructured porous membrane and the at least one protrusion being composed of materials suitable for the culture of two types of distinct cells; -- a support consisting of a non-absorbable membrane , including an upper face and a lower face , perforated by at least one perforation , said support being composed in particular of bioprinted plastic, polycarbonate, tissue culture plastic, glass or SU8, -- a 3D nanostructured porous membrane , including an upper face and a lower face , and including at least one protrusion , said at least one protrusion being hollow and including an outer face and an inner face and forming a structure in relief on the upper face side of the 3D nanostructured membrane , said protrusion being in particular in the shape of a hollow dome having a circular base , oo said lower face of said 3D nanostructured porous membrane being positioned in a rigidly connected manner on said upper face of said support , oo or said upper face of the 3D nanostructured membrane being positioned in a rigidly connected manner on said lower face of said support , oo and said 3D nanostructured porous membrane and the at least one protrusion being composed of materials suitable for the culture of two types of distinct cells; - a base , said central unit being integrated into said base , and forming a whole with said base . <EOS>