An integrated microfluidic device comprising: a sample fluid input chamber that provides an input of a sample fluid; a first overspill chamber in fluid communication with the sample fluid input chamber; a metering conduit in fluid communication with the sample fluid input chamber and the first overspill chamber, wherein the metering conduit meters, by capillarity, a first metered volume of fluid from the sample fluid, and the first overspill chamber receives fluid from the sample fluid in excess of the first metered volume of fluid; and a second overspill chamber in fluid communication with the metering conduit via a second overspill conduit , wherein the metering conduit meters, by capillarity, a second metered volume of fluid from the first metered volume of fluid, and the second overspill chamber receives fluid from the first metered volume of fluid in excess of the second metered volume of fluid; wherein the second overspill chamber has a fluid actuated closable valve for controlling the metering of the second metered volume of fluid and the first overspill chamber includes a first absorbent pad for absorbing the fluid in excess of the first metered volume of fluid, and the fluid actuated closable valve comprises a second absorbent pad for absorbing the fluid in excess of the second metered volume of fluid; wherein the second absorbent pad contains an absorbent material and an associated membrane, wherein the absorbent material is adapted to swell upon absorption of fluid thereby closing a hole in the membrane and thereby closing the valve to prevent additional fluid flow through the second overspill conduit . a sample fluid input chamber that provides an input of a sample fluid; a first overspill chamber in fluid communication with the sample fluid input chamber; a metering conduit in fluid communication with the sample fluid input chamber and the first overspill chamber, wherein the metering conduit meters, by capillarity, a first metered volume of fluid from the sample fluid, and the first overspill chamber receives fluid from the sample fluid in excess of the first metered volume of fluid; and a second overspill chamber in fluid communication with the metering conduit via a second overspill conduit , wherein the metering conduit meters, by capillarity, a second metered volume of fluid from the first metered volume of fluid, and the second overspill chamber receives fluid from the first metered volume of fluid in excess of the second metered volume of fluid; wherein the second overspill chamber has a fluid actuated closable valve for controlling the metering of the second metered volume of fluid and the first overspill chamber includes a first absorbent pad for absorbing the fluid in excess of the first metered volume of fluid, and the fluid actuated closable valve comprises a second absorbent pad for absorbing the fluid in excess of the second metered volume of fluid; wherein the second absorbent pad contains an absorbent material and an associated membrane, wherein the absorbent material is adapted to swell upon absorption of fluid thereby closing a hole in the membrane and thereby closing the valve to prevent additional fluid flow through the second overspill conduit . <EOS>