A device for delivery of therapeutic moieties to cells in a treatment zone of a tissue, said device comprising: a) a central probe defining at least a central lumen and extending from a proximal end to a distal end , at least a portion of said central probe defining a channel for delivery of said therapeutic moieties to said tissue, said portion of said central probe having at least one ejection port , wherein said proximal end of said central probe is open and fluidly connects said first central lumen with a lumen of an injector through which said therapeutic agent is delivered to said central probe , and wherein said distal end of said central probe is open to define an opening for delivery of said therapeutic moieties into said tissue at said treatment zone and has a shape configured to pierce said tissue; b) an applicator housing said central probe at least in part, said applicator having a distal end through which said portion of said central probe is configured to extend to an outside of said applicator to contact said tissue and to retract back into said applicator ; and c) an electroporation system comprising at least two electroporation electrodes configured to be oppositely charged and positioned surrounding said treatment zone , said electrodes being adapted to extend from proximal to distal ends, tips of said distal ends having a needle shape configured to pierce said tissue, wherein said electrodes are adapted to be coupled to an electrode power supply, receive at least one electrical waveform from said power supply, and supply a pulsed electric field sufficient for electroporation to said treatment zone , characterized in that the at least two electroporation electrodes are positioned around the central probe. a) a central probe defining at least a central lumen and extending from a proximal end to a distal end , at least a portion of said central probe defining a channel for delivery of said therapeutic moieties to said tissue, said portion of said central probe having at least one ejection port , wherein said proximal end of said central probe is open and fluidly connects said first central lumen with a lumen of an injector through which said therapeutic agent is delivered to said central probe , and wherein said distal end of said central probe is open to define an opening for delivery of said therapeutic moieties into said tissue at said treatment zone and has a shape configured to pierce said tissue; b) an applicator housing said central probe at least in part, said applicator having a distal end through which said portion of said central probe is configured to extend to an outside of said applicator to contact said tissue and to retract back into said applicator ; and c) an electroporation system comprising at least two electroporation electrodes configured to be oppositely charged and positioned surrounding said treatment zone , said electrodes being adapted to extend from proximal to distal ends, tips of said distal ends having a needle shape configured to pierce said tissue, wherein said electrodes are adapted to be coupled to an electrode power supply, receive at least one electrical waveform from said power supply, and supply a pulsed electric field sufficient for electroporation to said treatment zone , characterized in that the at least two electroporation electrodes are positioned around the central probe. <EOS>