A method for preparing lipoprotein complexes, comprising: (a) cooling a starting suspension comprising a lipid component and a protein component from a temperature in a first temperature range to a temperature in a second temperature range, wherein: (i) the lipid component consists essentially of particles of lipids; (ii) the protein component consists essentially of apolipoproteins and/or apolipoprotein mimetic peptides and, optionally, a lipid in an amount that is 10% or less by weight of the total amount of lipid in the starting suspension; (iii) the lipid component contains at least one neutral phospholipid; (iv) the lipid protein molar ratio in said starting suspension is from about 2:1 to about 200:1, the moles of apolipoproteins and/or apolipoprotein mimetic peptides defined in ApoA-I equivalents; and (v) the first temperature range includes temperatures from 12°C below to 12°C above the phase transition temperature of the protein component and the second temperature range includes temperatures at least about 20°C lower to about 40°C lower than the first temperature range; (b) heating the cooled suspension of (a) from a temperature in said second temperature range to a temperature in said first temperature range; (c) cooling said heated suspension of (b) from a temperature in said first temperature range to a temperature in said second temperature range; and (d) repeating steps (b) and (c) until at least 80% of the protein component is incorporated into lipoprotein complexes, thereby forming lipoprotein complexes. (a) cooling a starting suspension comprising a lipid component and a protein component from a temperature in a first temperature range to a temperature in a second temperature range, wherein: (i) the lipid component consists essentially of particles of lipids; (ii) the protein component consists essentially of apolipoproteins and/or apolipoprotein mimetic peptides and, optionally, a lipid in an amount that is 10% or less by weight of the total amount of lipid in the starting suspension; (iii) the lipid component contains at least one neutral phospholipid; (iv) the lipid protein molar ratio in said starting suspension is from about 2:1 to about 200:1, the moles of apolipoproteins and/or apolipoprotein mimetic peptides defined in ApoA-I equivalents; and (v) the first temperature range includes temperatures from 12°C below to 12°C above the phase transition temperature of the protein component and the second temperature range includes temperatures at least about 20°C lower to about 40°C lower than the first temperature range; (i) the lipid component consists essentially of particles of lipids; (ii) the protein component consists essentially of apolipoproteins and/or apolipoprotein mimetic peptides and, optionally, a lipid in an amount that is 10% or less by weight of the total amount of lipid in the starting suspension; (iii) the lipid component contains at least one neutral phospholipid; (iv) the lipid protein molar ratio in said starting suspension is from about 2:1 to about 200:1, the moles of apolipoproteins and/or apolipoprotein mimetic peptides defined in ApoA-I equivalents; and (v) the first temperature range includes temperatures from 12°C below to 12°C above the phase transition temperature of the protein component and the second temperature range includes temperatures at least about 20°C lower to about 40°C lower than the first temperature range; (b) heating the cooled suspension of (a) from a temperature in said second temperature range to a temperature in said first temperature range; (c) cooling said heated suspension of (b) from a temperature in said first temperature range to a temperature in said second temperature range; and (d) repeating steps (b) and (c) until at least 80% of the protein component is incorporated into lipoprotein complexes, <EOS>