An automated bioreactor system for decellularizing an organ, comprising: a main chamber configured to contain the organ, the main chamber having at least one reagent inlet , at least one reagent outlet , at least one perfusion inlet separate from the reagent inlet, and two waste outlets ; at least one reagent chamber configured to hold a liquid phase reagent; at least one reagent conduit configured to deliver the liquid phase reagent from the at least one reagent chamber to the main chamber through the at least one reagent inlet; at least one perfusion conduit configured to deliver the liquid phase reagent from the at least one reagent outlet into the organ contained in the main chamber through the at least one perfusion inlet; at least one perfusion pump configured to drive the flow of the liquid phase reagent through the at least one perfusion conduit; at least one perfusion pressure sensor configured to detect a pressure of the liquid phase reagent flowing through the at least one perfusion conduit; at least one drain conduit configured to deliver waste fluid from the main chamber to a drain ; and a control system configured to: receive an input representative of a desired pressure of the liquid phase reagent flowing through the at least one perfusion conduit; receive an input of the pressure detected by the at least one perfusion pressure sensor; and output a signal to control the at least one perfusion pump to drive the flow of the liquid phase reagent based on the received input representative of the desired pressure and the received input of the detected pressure. a main chamber configured to contain the organ, the main chamber having at least one reagent inlet , at least one reagent outlet , at least one perfusion inlet separate from the reagent inlet, and two waste outlets ; at least one reagent chamber configured to hold a liquid phase reagent; at least one reagent conduit configured to deliver the liquid phase reagent from the at least one reagent chamber to the main chamber through the at least one reagent inlet; at least one perfusion conduit configured to deliver the liquid phase reagent from the at least one reagent outlet into the organ contained in the main chamber through the at least one perfusion inlet; at least one perfusion pump configured to drive the flow of the liquid phase reagent through the at least one perfusion conduit; at least one perfusion pressure sensor configured to detect a pressure of the liquid phase reagent flowing through the at least one perfusion conduit; at least one drain conduit configured to deliver waste fluid from the main chamber to a drain ; and a control system configured to: receive an input representative of a desired pressure of the liquid phase reagent flowing through the at least one perfusion conduit; receive an input of the pressure detected by the at least one perfusion pressure sensor; and output a signal to control the at least one perfusion pump to drive the flow of the liquid phase reagent based on the received input representative of the desired pressure and the received input of the detected pressure. receive an input representative of a desired pressure of the liquid phase reagent flowing through the at least one perfusion conduit; receive an input of the pressure detected by the at least one perfusion pressure sensor; and output a signal to control the at least one perfusion pump to drive the flow of the liquid phase reagent based on the received input representative of the desired pressure and the received input of the detected pressure. <EOS>