Chromatography device, comprising at least: - a functional substrate , including a channel extending between a first (3-1) and a second (3-2) orifice opening onto a first face of the functional substrate, the channel comprising an inner wall covered with a functionalisation layer, - said first face of the functional substrate being disposed against a first face of a connection substrate , including a third (7-1) and a fourth (7-2) orifice opening onto said first face of the connection substrate, so that the first (3-1) and second (3-2) orifice are disposed respectively facing the third (7-1) and fourth (7-2) orifice, - the connection substrate being connected to a first and a second capillary respectively glued in a first (9-1) and a second (9-2) connection zone, the first (9-1) and second (9-2) connection zone opening onto an edge of the connection substrate , the connection substrate including a first fluid circuit extending between the first capillary and the third orifice and including a second fluid circuit extending between the second capillary and the fourth orifice, the connection substrate including a hood or cover assembled onto a part of the connection substrate in which the fluid circuits are made to close in a sealed manner the fluid connection zones (9-1, 9-2) and the fluid circuits ; and - a maintaining means adapted to maintain in a temporary manner said first face of the functional substrate bearing against said first face of the connection substrate, so as to allow the sealed passage of a fluid between the first and second capillaries, through the first fluid circuit, the channel of the functional substrate and the second fluid circuit. - a functional substrate , including a channel extending between a first (3-1) and a second (3-2) orifice opening onto a first face of the functional substrate, the channel comprising an inner wall covered with a functionalisation layer, - said first face of the functional substrate being disposed against a first face of a connection substrate , including a third (7-1) and a fourth (7-2) orifice opening onto said first face of the connection substrate, so that the first (3-1) and second (3-2) orifice are disposed respectively facing the third (7-1) and fourth (7-2) orifice, - the connection substrate being connected to a first and a second capillary respectively glued in a first (9-1) and a second (9-2) connection zone, the first (9-1) and second (9-2) connection zone opening onto an edge of the connection substrate , the connection substrate including a first fluid circuit extending between the first capillary and the third orifice and including a second fluid circuit extending between the second capillary and the fourth orifice, the connection substrate including a hood or cover assembled onto a part of the connection substrate in which the fluid circuits are made to close in a sealed manner the fluid connection zones (9-1, 9-2) and the fluid circuits ; and - a maintaining means adapted to maintain in a temporary manner said first face of the functional substrate bearing against said first face of the connection substrate, so as to allow the sealed passage of a fluid between the first and second capillaries, through the first fluid circuit, the channel of the functional substrate and the second fluid circuit. <EOS>