A method for nucleic acid sequencing, comprising: (a) providing a plurality of droplets, wherein a droplet of said plurality of droplets comprises (i) a ribonucleic acid (RNA) molecule from a cell comprising a nucleic acid sequence corresponding to an immunoglobulin variable region of a genome of said cell, (ii) a bead comprising a nucleic acid barcode molecule coupled thereto, wherein said nucleic acid barcode molecule comprises a barcode sequence and a template switching sequence, and (iii) a primer; (b) in said droplet, generating a barcoded nucleic acid molecule comprising, from a 5'end to a 3'end, a sequence corresponding to said nucleic acid sequence of said RNA molecule and a complement of said barcode sequence by (i) hybridizing said primer to a region at a 3' end of said RNA molecule, (ii) extending said primer to generate a nucleic acid product comprising a sequence at a 3' end that is complementary to the template switching sequence of said nucleic acid barcode molecule, (iii) hybridizing said nucleic acid product to said nucleic acid barcode molecule, and (iv) using said nucleic acid barcode molecule as a template to extend said nucleic acid product; (c) pooling said barcoded nucleic acid molecule from said droplet with other barcoded nucleic acid molecules from other droplets to provide a plurality of pooled barcoded nucleic acid molecules; (d) from a portion of said pooled barcoded nucleic acid molecules, amplifying said nucleic acid sequence corresponding to an immunoglobulin variable region to provide an enriched subset of barcoded nucleic acid molecules or derivatives thereof; and (e) sequencing said enriched subset of barcoded nucleic acid molecules or derivatives thereof and an unenriched subset of barcoded nucleic acid molecules from said plurality of pooled barcoded nucleic acid molecules. (a) providing a plurality of droplets, wherein a droplet of said plurality of droplets comprises (i) a ribonucleic acid (RNA) molecule from a cell comprising a nucleic acid sequence corresponding to an immunoglobulin variable region of a genome of said cell, (ii) a bead comprising a nucleic acid barcode molecule coupled thereto, wherein said nucleic acid barcode molecule comprises a barcode sequence and a template switching sequence, and (iii) a primer; (i) a ribonucleic acid (RNA) molecule from a cell comprising a nucleic acid sequence corresponding to an immunoglobulin variable region of a genome of said cell, (ii) a bead comprising a nucleic acid barcode molecule coupled thereto, wherein said nucleic acid barcode molecule comprises a barcode sequence and a template switching sequence, and (iii) a primer; (b) in said droplet, generating a barcoded nucleic acid molecule comprising, from a 5'end to a 3'end, a sequence corresponding to said nucleic acid sequence of said RNA molecule and a complement of said barcode sequence by (i) hybridizing said primer to a region at a 3' end of said RNA molecule, (ii) extending said primer to generate a nucleic acid product comprising a sequence at a 3' end that is complementary to the template switching sequence of said nucleic acid barcode molecule, (iii) hybridizing said nucleic acid product to said nucleic acid barcode molecule, and (iv) using said nucleic acid barcode molecule as a template to extend said nucleic acid product; (c) pooling said barcoded nucleic acid molecule from said droplet with other barcoded nucleic acid molecules from other droplets to provide a plurality of pooled barcoded nucleic acid molecules; (d) from a portion of said pooled barcoded nucleic acid molecules, amplifying said nucleic acid sequence corresponding to an immunoglobulin variable region to provide an enriched subset of barcoded nucleic acid molecules or derivatives thereof; and (e) sequencing said enriched subset of barcoded nucleic acid molecules or derivatives thereof and an unenriched subset of barcoded nucleic acid molecules from said plurality of pooled barcoded nucleic acid molecules. <EOS>