Method for manufacturing a microfluidic device comprising a microfluidic network comprising several microfluidic capsules in series, each microfluidic capsule having a chamber having a non-zero internal volume and which is arranged to control the flow of a fluid between an inlet channel and an outlet channel of the fluidic network, each microfluidic capsule being produced by the following steps: - producing a through-hole through a first card so as to define a cavity , said cavity having an inlet emerging on a first face of the first card, called top face, and an outlet emerging on a second face of the first card; - at least partially filling said cavity by the addition, via the inlet of said cavity, of a determined volume of an element made of deformable material initially taking the form of a liquid in order to delimit on the surface a deformable wall of said chamber and, opposite, a rear face of a command network of the capsule; - controlling said internal volume of the chamber by gauging the element made of deformable material added in the cavity to determine a concave form of said deformable wall and the depth of said wall; - solidifying said element made of deformable material inside the cavity so as to create said deformable wall delimiting said chamber, and in that the method also comprises steps of: - assembling a second card on said first card , so as to cover the inlet of each cavity , said second card comprising said microfluidic network comprising said inlet channel and said outlet channel associated with each capsule and arranged to communicate with the chamber of the capsule, - assembling a third card on said first card , so as to cover the outlet of said cavity, said third card comprising several actuation holes each emerging at a distinct microfluidic capsule. - producing a through-hole through a first card so as to define a cavity , said cavity having an inlet emerging on a first face of the first card, called top face, and an outlet emerging on a second face of the first card; - at least partially filling said cavity by the addition, via the inlet of said cavity, of a determined volume of an element made of deformable material initially taking the form of a liquid in order to delimit on the surface a deformable wall of said chamber and, opposite, a rear face of a command network of the capsule; - controlling said internal volume of the chamber by gauging the element made of deformable material added in the cavity to determine a concave form of said deformable wall and the depth of said wall; - solidifying said element made of deformable material inside the cavity so as to create said deformable wall delimiting said chamber, and in that the method also comprises steps of: - assembling a second card on said first card , so as to cover the inlet of each cavity , said second card comprising said microfluidic network comprising said inlet channel and said outlet channel associated with each capsule and arranged to communicate with the chamber of the capsule, - assembling a third card on said first card , so as to cover the outlet of said cavity, said third card comprising several actuation holes each emerging at a distinct microfluidic capsule. <EOS>