An in vitro method of profiling the energetic metabolism profile of a population of cells comprising: i) providing four samples [S1], [S2], [S3] and [S4] of said population of cells ii) measuring the protein synthesis level [LCo] in sample [S1] in absence of any inhibitor; iii) contacting the sample [S2] with an inhibitor [A] of the production of the energy resulting from glycolysis and the oxidative phosphorylation of glucose-derived pyruvate and measuring the protein synthesis level [LA] in said sample; iv) contacting the sample [S3] with an inhibitor [B] of the production of the energy resulting from TCA cycle and oxidative phosphorylation comprising pyruvate oxidation, oxidation of fatty acids and oxidation of amino acids and measuring the protein synthesis level [LB] in said sample; v) contacting the sample [S4] cells with both inhibitors [A] and [B] and measuring the protein synthesis level [L(A+B)] in said sample vi) assessing the glucose dependency of the population of cells; wherein the glucose dependency of the cells is assessed by calculating the formula (I): Glucose dependency = LCo − LA / LCo − L A + B × 100 vii) assessing the mitochondrial dependency of the population of cells; wherein the mitochondrial dependency of the cells is assessed by calculating the formula (II): Mitochondrial dependency = LCo − LB / LCo − L A + B × 100 viii) assessing the glycolytic capacity of the population of cells; wherein the glycolytic capacity of the cells is assessed by calculating the formula (III): Glycolytic capacity = (1 - ([LCo]-[LB]) / ([LCo]-[L(A+B)])) × 100 ix) assessing the capacity for oxidation of fatty acids and oxidation of amino acids of the population of cells, wherein the oxidation of fatty acids and oxidation of amino acids capacity is assessed by calculating the formula (IV): Oxidation of fatty acids & oxidation of amino acids capacity = (1 - ([LCo]-[LA]) / ([LCo]-[L(A+B)])) × 100 and x) finally determining the energetic metabolism profile of the population of cells. i) providing four samples [S1], [S2], [S3] and [S4] of said population of cells ii) measuring the protein synthesis level [LCo] in sample [S1] in absence of any inhibitor; iii) contacting the sample [S2] with an inhibitor [A] of the production of the energy resulting from glycolysis and the oxidative phosphorylation of glucose-derived pyruvate and measuring the protein synthesis level [LA] in said sample; iv) contacting the sample [S3] with an inhibitor [B] of the production of the energy resulting from TCA cycle and oxidative phosphorylation comprising pyruvate oxidation, oxidation of fatty acids and oxidation of amino acids and measuring the protein synthesis level [LB] in said sample; v) contacting the sample [S4] cells with both inhibitors [A] and [B] and measuring the protein synthesis level [L(A+B)] in said sample vi) assessing the glucose dependency of the population of cells; wherein the glucose dependency of the cells is assessed by calculating the formula (I): Glucose dependency = LCo − LA / LCo − L A + B × 100 vii) assessing the mitochondrial dependency of the population of cells; wherein the mitochondrial dependency of the cells is assessed by calculating the formula (II): Mitochondrial dependency = LCo − LB / LCo − L A + B × 100 viii) assessing the glycolytic capacity of the population of cells; wherein the glycolytic capacity of the cells is assessed by calculating the formula (III): Glycolytic capacity = (1 - ([LCo]-[LB]) / ([LCo]-[L(A+B)])) × 100 ix) assessing the capacity for oxidation of fatty acids and oxidation of amino acids of the population of cells, wherein the oxidation of fatty acids and oxidation of amino acids capacity is assessed by calculating the formula (IV): Oxidation of fatty acids & oxidation of amino acids capacity = (1 - ([LCo]-[LA]) / ([LCo]-[L(A+B)])) × 100 and x) finally determining the energetic metabolism profile of the population of cells. <EOS>