A method for the isolation of at least one first particle of a given type by means of a microfluidic system , which comprises a feeding assembly and a microfluidic circuit having: at least one inlet ; at least one first outlet ; and at least one microfluidic channel , which is designed to fluidically connect the inlet and the first outlet ; the microfluidic channel comprises at least one first segment , at least one second segment , which is arranged downstream of the first segment , and at least one third segment , which is arranged downstream of the second segment ; the method comprises a feeding step, during which the feeding assembly feeds a sample, comprising at least one first particle and at least one second particle of a type different from the first particle , from the inlet to the first outlet along the microfluidic channel ; and a trapping step, during which the first particle is trapped in and prevented from escaping from the second segment , while letting the second particle , which reaches at least the third segment , pass through the second segment ; the microfluidic system comprises a movement device , which is configured to directly exert a selective force upon said first particle of a given type; the method further comprises a selection step, which is subsequent to the trapping step and during which the movement device directly exerts a force upon the first particle so as to substantially selectively move the first particle , relative to at least part of a further content of the microfluidic channel , along at least one part of a given path (P) from the second segment downstream of the second segment itself; the given path (P) extends inside said microfluidic circuit ; wherein the microfluidic system comprises a collection area , which is fluidically connected to the microfluidic channel ; during the selection step, the movement device moves the first particle substantially selectively from the microfluidic channel , from the second segment , to the collection area . the microfluidic channel comprises at least one first segment , at least one second segment , which is arranged downstream of the first segment , and at least one third segment , which is arranged downstream of the second segment ; the method comprises a feeding step, during which the feeding assembly feeds a sample, comprising at least one first particle and at least one second particle of a type different from the first particle , from the inlet to the first outlet along the microfluidic channel ; and a trapping step, during which the first particle is trapped in and prevented from escaping from the second segment , while letting the second particle , which reaches at least the third segment , pass through the second segment ; the microfluidic system comprises a movement device , which is configured to directly exert a selective force upon said first particle of a given type; the method further comprises a selection step, which is subsequent to the trapping step and during which the movement device directly exerts a force upon the first particle so as to substantially selectively move the first particle , relative to at least part of a further content of the microfluidic channel , along at least one part of a given path (P) from the second segment downstream of the second segment itself; the given path (P) extends inside said microfluidic circuit ; wherein the microfluidic system comprises a collection area , which is fluidically connected to the microfluidic channel ; during the selection step, the movement device moves the first particle substantially selectively from the microfluidic channel , from the second segment , to the collection area . <EOS>