A system for seeding cells comprising: a cellular isolate fluid container ; a flow channel disposed between the cellular isolate fluid container , a collection fluid container , an elution fluid container , and a seeding container , wherein the flow channel comprises an inlet, an outlet and a filter therebetween, the collection fluid container comprises an inlet port and an outlet port adapted for the sterile filling and/or dispensing of fluid, the cellular isolate fluid container comprises at least one port adapted for the sterile filling and/or dispensing of fluid, the elution fluid container comprises at least one port adapted for the sterile filling and/or dispensing of fluid, and the seeding container comprises at least one port adapted for the sterile filling and/or dispensing of fluid; wherein the filter is adapted to allow flow in at least two directions, and wherein the flow channel is selectively in fluid communication with each of the cellular isolate fluid container , the collection fluid container , the elution fluid container , and the seeding container , respectively; and wherein the seeding container comprises a cell seeding assembly , wherein the cell seeding assembly comprises a porous tube and a scaffold ; wherein the cellular isolate fluid container is in fluid communication with the collection fluid container via the flow channel , fluid lines , and at least one valve ; wherein the collection fluid container is in fluid communication with the seeding container via fluid lines and at least one valve ; and wherein the elution fluid container is in fluid communication with the seeding container via fluid lines , the flow channel , and at least one valve . a cellular isolate fluid container ; a flow channel disposed between the cellular isolate fluid container , a collection fluid container , an elution fluid container , and a seeding container , wherein the flow channel comprises an inlet, an outlet and a filter therebetween, the collection fluid container comprises an inlet port and an outlet port adapted for the sterile filling and/or dispensing of fluid, the cellular isolate fluid container comprises at least one port adapted for the sterile filling and/or dispensing of fluid, the elution fluid container comprises at least one port adapted for the sterile filling and/or dispensing of fluid, and the seeding container comprises at least one port adapted for the sterile filling and/or dispensing of fluid; wherein the filter is adapted to allow flow in at least two directions, and wherein the flow channel is selectively in fluid communication with each of the cellular isolate fluid container , the collection fluid container , the elution fluid container , and the seeding container , respectively; and wherein the seeding container comprises a cell seeding assembly , wherein the cell seeding assembly comprises a porous tube and a scaffold ; wherein the cellular isolate fluid container is in fluid communication with the collection fluid container via the flow channel , fluid lines , and at least one valve ; wherein the collection fluid container is in fluid communication with the seeding container via fluid lines and at least one valve ; and wherein the elution fluid container is in fluid communication with the seeding container via fluid lines , the flow channel , and at least one valve . <EOS>