Method of high-resolution imaging a structure of a sample , the structure being marked with fluorescence markers, - wherein the sample is subjected to a light intensity distribution in which an intensity maximum of focused fluorescence excitation light is superimposed with an intensity minimum enclosed by intensity maxima of focused fluorescence inhibition light , - wherein selectively partial areas of interest of the sample are scanned with the intensity minimum of the focused fluorescence inhibition light , wherein fluorescence light emitted out of the sample is registered and allocated to the respective location of the intensity minimum of the focused fluorescence inhibition light in the sample , and - wherein at least the subjection of the sample to the focused fluorescence inhibition light of the light intensity distribution is terminated for the respective location of the intensity minimum of the focused fluorescence inhibition light , - when a predetermined maximum light amount (LM4) of the fluorescence light emitted out of the sample has been registered, and/or - if, within a predetermined first period of time (Δt2), a predetermined minimum light amount (LM2) of the fluorescence light emitted out of the sample has not yet been registered, characterised in - that the sample is scanned with the focused fluorescence excitation light in steps adjusted to the dimensions of the intensity minimum of the focused fluorescence inhibition light , wherein fluorescence light emitted out of the sample is registered, and wherein the fluorescence inhibition light is only switched on in each step, if a predetermined minimum light amount (LM1) of the fluorescence light emitted out of the sample has been registered within a predetermined second trial period of time (Δt1). - wherein the sample is subjected to a light intensity distribution in which an intensity maximum of focused fluorescence excitation light is superimposed with an intensity minimum enclosed by intensity maxima of focused fluorescence inhibition light , - wherein selectively partial areas of interest of the sample are scanned with the intensity minimum of the focused fluorescence inhibition light , wherein fluorescence light emitted out of the sample is registered and allocated to the respective location of the intensity minimum of the focused fluorescence inhibition light in the sample , and - wherein at least the subjection of the sample to the focused fluorescence inhibition light of the light intensity distribution is terminated for the respective location of the intensity minimum of the focused fluorescence inhibition light , - when a predetermined maximum light amount (LM4) of the fluorescence light emitted out of the sample has been registered, and/or - if, within a predetermined first period of time (Δt2), a predetermined minimum light amount (LM2) of the fluorescence light emitted out of the sample has not yet been registered, characterised in - when a predetermined maximum light amount (LM4) of the fluorescence light emitted out of the sample has been registered, and/or - if, within a predetermined first period of time (Δt2), a predetermined minimum light amount (LM2) of the fluorescence light emitted out of the sample has not yet been registered, - that the sample is scanned with the focused fluorescence excitation light in steps adjusted to the dimensions of the intensity minimum of the focused fluorescence inhibition light , wherein fluorescence light emitted out of the sample is registered, and wherein the fluorescence inhibition light is only switched on in each step, if a predetermined minimum light amount (LM1) of the fluorescence light emitted out of the sample has been registered within a predetermined second trial period of time (Δt1). <EOS>