A method of determining a ratio of glycated hemoglobin to total hemoglobin in a whole blood sample, the method comprising the steps of: applying a whole blood sample on an upper surface of a blood separation membrane of a microfluidics device , the blood separation membrane having a lower surface that is associated with a first waste collection material , whereby red blood cells in the whole blood sample are captured on and/or in the blood separation membrane while plasma passes through the blood separation membrane and is wicked away from the blood separation membrane and into the first waste collection material ; moving a portion of the microfluidics device so that the blood separation membrane is positioned over a negatively charged surface having a second waste collection material positioned therebelow and in association therewith; applying a lysing agent to the blood separation membrane , wherein the contents of the lysed red blood cells pass through the blood separation membrane and are brought into contact with the negatively charged surface , and wherein the negatively charged surface traps hemoglobin thereon and/or therein while the other components of lysed red blood cells are wicked away from the negatively charged surface and into the second waste collection material ; moving a portion of the microfluidics device so that the negatively charged surface is positioned within an optical read chamber that allows for optical interrogation of the negatively charged surface by a spectrometer; spectroscopically measuring an amount of total hemoglobin on and/or in the negatively charged surface and an amount of one of glycated hemoglobin or non-glycated hemoglobin on and/or in the negatively charged surface ; and determining a ratio of glycated hemoglobin to total hemoglobin in the whole blood sample based on the spectroscopic measurements. applying a whole blood sample on an upper surface of a blood separation membrane of a microfluidics device , the blood separation membrane having a lower surface that is associated with a first waste collection material , whereby red blood cells in the whole blood sample are captured on and/or in the blood separation membrane while plasma passes through the blood separation membrane and is wicked away from the blood separation membrane and into the first waste collection material ; moving a portion of the microfluidics device so that the blood separation membrane is positioned over a negatively charged surface having a second waste collection material positioned therebelow and in association therewith; applying a lysing agent to the blood separation membrane , wherein the contents of the lysed red blood cells pass through the blood separation membrane and are brought into contact with the negatively charged surface , and wherein the negatively charged surface traps hemoglobin thereon and/or therein while the other components of lysed red blood cells are wicked away from the negatively charged surface and into the second waste collection material ; moving a portion of the microfluidics device so that the negatively charged surface is positioned within an optical read chamber that allows for optical interrogation of the negatively charged surface by a spectrometer; spectroscopically measuring an amount of total hemoglobin on and/or in the negatively charged surface and an amount of one of glycated hemoglobin or non-glycated hemoglobin on and/or in the negatively charged surface ; and determining a ratio of glycated hemoglobin to total hemoglobin in the whole blood sample based on the spectroscopic measurements. <EOS>