A method to obtain the spatial location and sequence information of a target sequence of at least one m-RNA strand on a tissue sample comprising the steps a. providing a linear probe, containing a) a binding region capable of binding to the at least one m-RNA strand and b) an anchor sequence comprising a UMI region located between a first and a second locator regions and c) a primer region; b. hybridizing the linear probe with its binding region to the m-RNA strand; c. complementing the linear probe using the m-RNA strand as template thereby obtaining a reversed transcribed c-DNA strand d. hybridizing a locator molecule with its 3' and 5' ends to the first and second locator regions thereby creating a gap corresponding to the length of the UMI of the linear probe e. Filling the gap in the locator molecule with nucleotides complementary to the UMI using a non-strand displacement enzyme thereby creating a circular template comprising a copy of the UMI region from the linear probe. f. multiplying the circular template molecule by RCA on the tissue sample, starting from a primer region thereby creating a rolony g. Sequencing at least the UMI portion of the rolonies thereby obtaining the spatial location of the m-RNA on the tissue h. removing the reversed transcribed c-DNA strand from the tissue and dehybridizing the m-RNA strand thereby obtaining a single stranded c-DNA oligomer i. providing the single stranded cDNA oligomer with a first and a second adaptor primer at the 3' and 5' ends obtaining a primed single stranded oligomer; amplification of the primed single stranded oligomer by PCR j. Sequencing the amplified primed single stranded oligomer and linking the spatial information of the rolonies with the sequence information of the amplified primed single stranded oligomer via the UMI sequence a. providing a linear probe, containing a) a binding region capable of binding to the at least one m-RNA strand and b) an anchor sequence comprising a UMI region located between a first and a second locator regions and c) a primer region; b. hybridizing the linear probe with its binding region to the m-RNA strand; c. complementing the linear probe using the m-RNA strand as template thereby obtaining a reversed transcribed c-DNA strand d. hybridizing a locator molecule with its 3' and 5' ends to the first and second locator regions thereby creating a gap corresponding to the length of the UMI of the linear probe e. Filling the gap in the locator molecule with nucleotides complementary to the UMI using a non-strand displacement enzyme thereby creating a circular template comprising a copy of the UMI region from the linear probe. f. multiplying the circular template molecule by RCA on the tissue sample, starting from a primer region thereby creating a rolony g. Sequencing at least the UMI portion of the rolonies thereby obtaining the spatial location of the m-RNA on the tissue h. removing the reversed transcribed c-DNA strand from the tissue and dehybridizing the m-RNA strand thereby obtaining a single stranded c-DNA oligomer i. providing the single stranded cDNA oligomer with a first and a second adaptor primer at the 3' and 5' ends obtaining a primed single stranded oligomer; amplification of the primed single stranded oligomer by PCR j. Sequencing the amplified primed single stranded oligomer and linking the spatial information of the rolonies with the sequence information of the amplified primed single stranded oligomer via the UMI sequence <EOS>