A cell culture method comprising: culturing cells at 30 to 45°C in a medium in which a human-type recombinant protein is dissolved, wherein the human-type recombinant protein is laminin, collagen, gelatin, or a variant thereof, and the content of the human-type recombinant protein in the medium is 0.01 ng/mL to 100 μg/mL, wherein the human-type recombinant protein includes recombinant gelatin having an amino acid sequence derived from a partial amino acid sequence of collagen, wherein the human-type recombinant protein has a repeating sequence represented by Gly-X-Y characteristic to collagen, X and Y each independently represent any one of amino acid, a plurality of pieces of the Gly-X-Y may be the same as or different from each other, and the human-type recombinant protein includes two or more sequences of cell adhesion signals in one molecule, wherein the cell adhesion signal is an amino acid sequence represented by Arg-Gly-Asp, and wherein the amino acid sequence of the human-type recombinant protein is represented by the following formula, A-[(Gly-X-Y)n]m-B in the formula, A represents any amino acid or an amino acid sequence, B represents any amino acid or an amino acid sequence, n pieces of X each independently represent any amino acid, n pieces of Y each independently represent any amino acid, and n represents an integer of 3 to 100, m represents an integer of 2 to 10, and n pieces of Gly-X-Y may be the same as or different from each other, wherein the cells are mesenchymal stem cells, and wherein the human-type recombinant protein has an amino acid sequence described in SEQ ID No: 1, or an amino acid sequence having 80% or more sequence identity to the amino acid sequence described in SEQ ID No: 1 and has cell adhesiveness. culturing cells at 30 to 45°C in a medium in which a human-type recombinant protein is dissolved, wherein the human-type recombinant protein is laminin, collagen, gelatin, or a variant thereof, and the content of the human-type recombinant protein in the medium is 0.01 ng/mL to 100 μg/mL, wherein the human-type recombinant protein includes recombinant gelatin having an amino acid sequence derived from a partial amino acid sequence of collagen, wherein the human-type recombinant protein has a repeating sequence represented by Gly-X-Y characteristic to collagen, X and Y each independently represent any one of amino acid, a plurality of pieces of the Gly-X-Y may be the same as or different from each other, and the human-type recombinant protein includes two or more sequences of cell adhesion signals in one molecule, wherein the cell adhesion signal is an amino acid sequence represented by Arg-Gly-Asp, and wherein the amino acid sequence of the human-type recombinant protein is represented by the following formula, A-[(Gly-X-Y)n]m-B in the formula, A represents any amino acid or an amino acid sequence, B represents any amino acid or an amino acid sequence, n pieces of X each independently represent any amino acid, n pieces of Y each independently represent any amino acid, and n represents an integer of 3 to 100, m represents an integer of 2 to 10, and n pieces of Gly-X-Y may be the same as or different from each other, wherein the cells are mesenchymal stem cells, and wherein the human-type recombinant protein has an amino acid sequence described in SEQ ID No: 1, or an amino acid sequence having 80% or more sequence identity to the amino acid sequence described in SEQ ID No: 1 and has cell adhesiveness. <EOS>