An acoustic loading system for transporting an analyte in a fluid sample to an analytical instrument, comprising: (a) a reservoir housing a fluid sample containing an analyte, the fluid sample having a fluid surface ; (b) a continuous flow sampling probe spaced apart from the fluid surface , comprising (i) a sampling tip for receiving an ejected droplet of the fluid sample , (ii) a solvent inlet for receiving a solvent from a solvent source , (iii) a solvent transport capillary for transporting the solvent from the solvent inlet to the sampling tip , where the ejected droplet combines with the solvent to form an analyte- solvent dilution, (iv) a sample outlet through which the analyte- solvent dilution is directed away from the sampling probe to an analytical instrument, and (v) a sample transport capillary for transporting the analyte- solvent dilution from the sampling tip to the sample outlet , wherein the sample transport capillary and the solvent transport capillary are in fluid communication at the sampling tip ; and (c) an acoustic droplet ejector for generating acoustic radiation in a manner effective to successively eject discrete fluid sample droplets from the fluid surface into the sampling tip of the sampling probe ; wherein the acoustic droplet ejector comprises an acoustic radiation generator and a focusing means for focusing the acoustic radiation generated at a focal point near the surface of a fluid sample in the reservoir , and further wherein the acoustic droplet ejector is in acoustic coupling relationship with the reservoir . (a) a reservoir housing a fluid sample containing an analyte, the fluid sample having a fluid surface ; (b) a continuous flow sampling probe spaced apart from the fluid surface , comprising (i) a sampling tip for receiving an ejected droplet of the fluid sample , (ii) a solvent inlet for receiving a solvent from a solvent source , (iii) a solvent transport capillary for transporting the solvent from the solvent inlet to the sampling tip , where the ejected droplet combines with the solvent to form an analyte- solvent dilution, (iv) a sample outlet through which the analyte- solvent dilution is directed away from the sampling probe to an analytical instrument, and (v) a sample transport capillary for transporting the analyte- solvent dilution from the sampling tip to the sample outlet , wherein the sample transport capillary and the solvent transport capillary are in fluid communication at the sampling tip ; and (i) a sampling tip for receiving an ejected droplet of the fluid sample , (ii) a solvent inlet for receiving a solvent from a solvent source , (iii) a solvent transport capillary for transporting the solvent from the solvent inlet to the sampling tip , where the ejected droplet combines with the solvent to form an analyte- solvent dilution, (iv) a sample outlet through which the analyte- solvent dilution is directed away from the sampling probe to an analytical instrument, and (v) a sample transport capillary for transporting the analyte- solvent dilution from the sampling tip to the sample outlet , wherein the sample transport capillary and the solvent transport capillary are in fluid communication at the sampling tip ; and (c) an acoustic droplet ejector for generating acoustic radiation in a manner effective to successively eject discrete fluid sample droplets from the fluid surface into the sampling tip of the sampling probe ; <EOS>