Arrangement for light sheet microscopy, comprising - a sample vessel for holding a sample located in a medium , wherein the sample vessel has a covering and is oriented with respect to a planar reference surface, - an illumination optical unit having an illumination objective , which is configured to illuminate the sample with a light sheet, wherein the optical axis of the illumination objective and the light sheet are situated in a plane that encloses an illumination angle β that differs from zero with the normal of the reference surface, - a detection optical unit having a detection objective , the optical axis of which encloses a detection angle δ that differs from zero with the normal of the reference surface, - at least one protrusion, which is transparent for illumination and detection light and has an inner boundary surface and an outer boundary surface for holding a sample in said protrusion, is formed on the cover, - the shape of the at least one protrusion, the position thereof upon observation and the position of the optical axes of the illumination objective and the detection objective are adapted to one another by virtue of the fact that the optical axes enclose with the normals of the boundary surfaces an angle of 0° to 5° at least in the region where the optical axes penetrate the boundary surfaces, characterized in that - the at least one protrusion comprises two plate-type elements which protrude from the cover and from the sample vessel, have parallel boundary surfaces and are in contact in at least one point at the location where the protrusion has the greatest distance from the remaining sample vessel , wherein the normals of the boundary surfaces of a first plate-type element coincide with the optical axis of the illumination objective and the normals of the boundary surfaces of a second plate-type element coincide with the optical axis of the detection objective , and wherein the sum of the illumination angle β and the detection angle δ is 90°. - a sample vessel for holding a sample located in a medium , wherein the sample vessel has a covering and is oriented with respect to a planar reference surface, - an illumination optical unit having an illumination objective , which is configured to illuminate the sample with a light sheet, wherein the optical axis of the illumination objective and the light sheet are situated in a plane that encloses an illumination angle β that differs from zero with the normal of the reference surface, - a detection optical unit having a detection objective , the optical axis of which encloses a detection angle δ that differs from zero with the normal of the reference surface, - at least one protrusion, which is transparent for illumination and detection light and has an inner boundary surface and an outer boundary surface for holding a sample in said protrusion, is formed on the cover, - the shape of the at least one protrusion, the position thereof upon observation and the position of the optical axes of the illumination objective and the detection objective are adapted to one another by virtue of the fact that the optical axes enclose with the normals of the boundary surfaces an angle of 0° to 5° at least in the region where the optical axes penetrate the boundary surfaces, characterized in that - the at least one protrusion comprises two plate-type elements which protrude from the cover and from the sample vessel, have parallel boundary surfaces and are in contact in at least one point at the location where the protrusion has the greatest distance from the remaining sample vessel , wherein the normals of the boundary surfaces of a first plate-type element coincide with the optical axis of the illumination objective and the normals of the boundary surfaces of a second plate-type element coincide with the optical axis of the detection objective , and wherein the sum of the illumination angle β and the detection angle δ is 90°. <EOS>