A method of removing entrapped gas, pressure, or both gas and pressure from a pre-packed chromatography column that comprises a column tube having a column tube inlet and a column tube outlet and first and second flow distributors arranged within the column tube to form a chamber filled with a packing medium, the method comprising: (a) obtaining a tubing and valve set comprising tubing and multiple valves configured to define at least two different fluid paths through the tubing; (b) either: (i) attaching tubing of the tubing and valve set to the column tube inlet and the column tube outlet and then sterilizing the connected pre-packed chromatography column and the tubing and valve set , or (ii) sterilizing the pre-packed chromatography column and the tubing and valve set individually and then attaching the sterile tubing of the tubing and valve set to the sterile column tube inlet and the sterile column tube outlet in a manner that maintains sterility of both the column and the tubing and valve set ; (c) attaching the sterile tubing and valve set to a sterile or aseptic fluid source and to a fluid outlet ; and (d) pumping sterile or aseptic liquid from the sterile or aseptic fluid source along the tubing and valve set , wherein the multiple valves is in a first position, into the column tube inlet and out from the column tube outlet along a first flow path, thereby removing any entrapped gas, pressure, or both gas and pressure from the chamber, the tubing, and the multiple valves along the first flow path without breaching the sterility of the pre-packed chromatography column and the tubing and valve set . (a) obtaining a tubing and valve set comprising tubing and multiple valves configured to define at least two different fluid paths through the tubing; (b) either: (i) attaching tubing of the tubing and valve set to the column tube inlet and the column tube outlet and then sterilizing the connected pre-packed chromatography column and the tubing and valve set , or (ii) sterilizing the pre-packed chromatography column and the tubing and valve set individually and then attaching the sterile tubing of the tubing and valve set to the sterile column tube inlet and the sterile column tube outlet in a manner that maintains sterility of both the column and the tubing and valve set ; (i) attaching tubing of the tubing and valve set to the column tube inlet and the column tube outlet and then sterilizing the connected pre-packed chromatography column and the tubing and valve set , or (ii) sterilizing the pre-packed chromatography column and the tubing and valve set individually and then attaching the sterile tubing of the tubing and valve set to the sterile column tube inlet and the sterile column tube outlet in a manner that maintains sterility of both the column and the tubing and valve set ; (c) attaching the sterile tubing and valve set to a sterile or aseptic fluid source and to a fluid outlet ; and (d) pumping sterile or aseptic liquid from the sterile or aseptic fluid source along the tubing and valve set , wherein the multiple valves is in a first position, into the column tube inlet and out from the column tube outlet along a first flow path, thereby removing any entrapped gas, pressure, or both gas and pressure from the chamber, the tubing, and the multiple valves along the first flow path without breaching the sterility of the pre-packed chromatography column and the tubing and valve set . <EOS>