A system for treatment of biological tissues comprising target tissues and other tissues, comprising: a radiation source configured for generating non-ionizing radiation; a pulse generator configured for generating a plurality of voltage pulses; a plurality of electrodes, coupled to the pulse generator and arranged so that an electric field resulting from the plurality of pulses and between the plurality of electrodes traverses at least a portion of the target tissues; at least one guide device for directing the non-ionizing radiation and the electromagnetic energy to at least the portion of the target tissues; a temperature probe for measuring a temperature of at least the portion of the target tissues; and a processor coupled to the radiation source, the pulse generator, and the temperature probe, the processor configured for causing the radiation source to generate the non-ionizing radiation to elevate and to maintain a temperature of at least the portion of the target tissues at a treatment temperature range which is above a physiological temperature of the biological tissue and at or below about 47° C, and causing the pulse generator to generate a pre-defined sequence of short voltage pulses while the treatment temperature range is maintained, wherein the pre-defined sequence is configured to cause the electric field to deliver electrical energy having a magnitude for inducing electromanipulation in the portion of the target tissues at the treatment temperature range, and wherein the delivered electrical energy is lower than a specific electrical energy sufficient to induce electromanipulation in the portion of the target tissue at or below the physiological temperature. a radiation source configured for generating non-ionizing radiation; a pulse generator configured for generating a plurality of voltage pulses; a plurality of electrodes, coupled to the pulse generator and arranged so that an electric field resulting from the plurality of pulses and between the plurality of electrodes traverses at least a portion of the target tissues; at least one guide device for directing the non-ionizing radiation and the electromagnetic energy to at least the portion of the target tissues; a temperature probe for measuring a temperature of at least the portion of the target tissues; and a processor coupled to the radiation source, the pulse generator, and the temperature probe, the processor configured for causing the radiation source to generate the non-ionizing radiation to elevate and to maintain a temperature of at least the portion of the target tissues at a treatment temperature range which is above a physiological temperature of the biological tissue and at or below about 47° C, and causing the pulse generator to generate a pre-defined sequence of short voltage pulses while the treatment temperature range is maintained, wherein the pre-defined sequence is configured to cause the electric field to deliver electrical energy having a magnitude for inducing electromanipulation in the portion of the target tissues at the treatment temperature range, and wherein the delivered electrical energy is lower than a specific electrical energy sufficient to induce electromanipulation in the portion of the target tissue at or below the physiological temperature. <EOS>