A microscope apparatus , comprising: a distribution measurement apparatus that is configured to obtain a fluorescent picture image by conducting irradiation with an exciting light with respect to an observation region and that is configured to measure a fluorescent intensity distribution of the observation region, the observation region being a region where a sample, which comprises a fluorescent material that is activated when irradiated with an activating light of a prescribed wavelength and which emits fluorescence when irradiated in an activated condition with the exciting light of a wavelength that differs from that of the activating light, is arranged; an irradiation intensity setting apparatus that is configured to set different irradiation intensities of activating light that irradiates the observation region based on the fluorescent intensity distribution; and a picture image formation apparatus that is configured to generate a sample picture image based on a plurality of fluorescent picture images, the plurality of fluorescent picture images being obtained by repeatedly obtaining a fluorescent picture image, the fluorescent picture image being obtained by irradiating the observation region with the activating light, set at the different irradiation intensities, and with the exciting light, characterized in that the irradiation intensities of the activating light, which irradiates the sample, is set to a relatively low intensity in first regions where the fluorescent intensity is relatively high in the fluorescent intensity distribution, and is set to a relatively high intensity in second regions where the fluorescent intensity is relatively low in the fluorescent intensity distribution, the irradiation intensities of the activating light is set lower in the first regions than in the second regions and fluorescent intensity is higher in the first regions than in the second regions. a distribution measurement apparatus that is configured to obtain a fluorescent picture image by conducting irradiation with an exciting light with respect to an observation region and that is configured to measure a fluorescent intensity distribution of the observation region, the observation region being a region where a sample, which comprises a fluorescent material that is activated when irradiated with an activating light of a prescribed wavelength and which emits fluorescence when irradiated in an activated condition with the exciting light of a wavelength that differs from that of the activating light, is arranged; an irradiation intensity setting apparatus that is configured to set different irradiation intensities of activating light that irradiates the observation region based on the fluorescent intensity distribution; and a picture image formation apparatus that is configured to generate a sample picture image based on a plurality of fluorescent picture images, the plurality of fluorescent picture images being obtained by repeatedly obtaining a fluorescent picture image, the fluorescent picture image being obtained by irradiating the observation region with the activating light, set at the different irradiation intensities, and with the exciting light, characterized in that the irradiation intensities of the activating light, which irradiates the sample, is set to a relatively low intensity in first regions where the fluorescent intensity is relatively high in the fluorescent intensity distribution, and is set to a relatively high intensity in second regions where the fluorescent intensity is relatively low in the fluorescent intensity distribution, the irradiation intensities of the activating light is set lower in the first regions than in the second regions and fluorescent intensity is higher in the first regions than in the second regions. <EOS>