A composition comprising the following proteins in an amount of at least about 2 times (fold change) higher than the same protein present in the whole tachyzoite extract, as calculated by quantitative label-free liquid chromatography-tandem mass spectrometry (LC-MS/MS), wherein the term "about" means the indicated value ± 30% of its value, preferably wherein the term "about" means the indicated value ± 20% of its value: Accession number 1 SEQ ID NO: Description 2 NCLIV_018120 177 conserved hypothetical protein NCLIV_003410 886 putative HECT-domain (ubiquitin-transferase) containing protein NCLIV_058550 347 conserved hypothetical protein NCLIV_042610 897 conserved hypothetical protein NCLIV_032910 382 hypothetical protein NCLIV_024830 519 conserved hypothetical protein NCLIV_015180 323 ATP synthase, related NCLIV_066970 453 putative enoyl-acyl carrier reductase NCLIV_025730 560 conserved hypothetical protein NCLIV_006640 289 hypothetical protein NCLIV_003470 887 putative thrombospondin type 1 domain-containing protein NCLIV_019000 390 putative adenosine transporter NCLIV_054510 785 putative heat shock protein 90 NCLIV_066350 577 Os06g0732000 protein, related NCLIV_057020 902 conserved hypothetical protein NCLIV_056680 246 hypothetical protein NCLIV_044290 463 pyruvate dehydrogenase E2 component, related NCLIV_032030 680 conserved hypothetical protein NCLIV_052240 362 putative saccharopine dehydrogenase NCLIV_008730 318 hypothetical protein NCLIV_061830 120 60S acidic ribosomal protein P0 NCLIV_002770 440 putative MORN repeat-containing protein NCLIV_014760 636 conserved hypothetical protein NCLIV_054520 322 hypothetical protein NCLIV_033810 716 hypothetical protein NCLIV_043330 121 hypothetical protein NCLIV_000430 486 conserved hypothetical protein NCLIV_030860 176 conserved hypothetical protein NCLIV_048570 58 conserved hypothetical protein NCLIV_004190 76 putative thioredoxin NCLIV_019450 459 hypothetical protein NCLIV_027160 185 conserved hypothetical protein NCLIV_044600 633 conserved hypothetical protein NCLIV_000300 307 conserved hypothetical protein NCLIV_046830 540 putative ATP synthase NCLIV_004280 315 hypothetical protein NCLIV_006720 229 conserved hypothetical protein NCLIV_046800 188 putative AGC kinase NCLIV_051960 769 conserved hypothetical protein NCLIV_010600 45 putative microneme protein MIC3 NCLIV_015920 87 Histone H4, related NCLIV_012830 285 putative MORN repeat-containing protein NCLIV_0376 1022 elongation factor Tu GTP-binding domain-containing protein NCLIV_024420 140 hypothetical protein NCLIV_036130 570 CBR-RSP-4 protein, related NCLIV_036400 156 unspecified product NCLIV_006780 397 conserved hypothetical protein NCLIV_046940 899 putative PWWP domain-containing protein NCLIV_001370 491 putative DEAD/DEAH box helicase NCLIV_001300 60 putative calmodulin NCLIV_015380 80 conserved hypothetical protein NCLIV_038990 895 conserved hypothetical protein NCLIV_043110 247 putative interferon gamma-inducible protein 30 1 Accession number for the identified protein in ToxoDB database (ToxoDB-13.0_NcaninumLIV_AnnotatedProteins; 7122 sequences; Date 09 February 2015). 2 Protein description in ToxoDB database (ToxoDB-13.0_NcaninumLIV_AnnotatedProteins; 7122 sequences; Date 09 February 2015). wherein the whole tachyzoite extract is prepared by direct resuspension of 10 8 frozen tachyzoites of the Nc-Spain 7 isolate of N. caninum, with deposit number CCAP 2051/1, in 100 μl of 1% Triton-X 100 (v/v). <EOS>