A production method for preparing the most nature identical cartilage tissue for use in cartilage tissue defects and cartilage tissue regeneration, characterized by the steps of - cultivating the mesenchymal stem cells (MSC) extracted from rat bone marrow in a sterile environment in cell culture dishes, - seeding the cultivated cells to cell culture dishes after being passaged, - in the meantime, amplifying the psPAX2 (lentiviral packaging plasmid), pMD2.G (lentiviral envelope plasmid) and pTGM2(V2) (expression plasmid) plasmids using E.coli bacteria, - confirming the sequence of hTGM2 (V2) gene in pTGM2(V2), - producing lentiviruses containing pTGM2 gene by delivering psPAX2, pMD2.G, pTGM2(V2) plasmids to HEK293T cells in triplets, - when the mesenchymal stem cells (MSC) are at about 70% confluency, performing transduction process of the mesenchymal stem cells by adding lentiviruses onto the mesenchymal stem cells (MSC) in the culture dishes, - incubating the mesenchymal stem cells (MSC) in the CO 2 incubator, - at the end of the incubation, performing PBS washing and then seeding in a normal medium and proceeding with the usual cell culture, - incubating the transduced cells in a medium containing Blasticidin antibiotic and eliminating the cells that has not taken up the transgene from the medium, - proving that living cells have successfully acquired the gene, - preparing poly(1,4-butylene succinate)/ poly-L-lactide (PLLA) (PBSu/PLLA) polymer tissue scaffolds by using salt leaching technique, - seeding the cells onto the prepared PBSu/PLLA polymer tissue scaffolds, - after waiting for adhesion of the cells to the tissue scaffolds, adding medium to the culture dishes, - obtaining the nature identical cartilage tissue. - cultivating the mesenchymal stem cells (MSC) extracted from rat bone marrow in a sterile environment in cell culture dishes, - seeding the cultivated cells to cell culture dishes after being passaged, - in the meantime, amplifying the psPAX2 (lentiviral packaging plasmid), pMD2.G (lentiviral envelope plasmid) and pTGM2(V2) (expression plasmid) plasmids using E.coli bacteria, - confirming the sequence of hTGM2 (V2) gene in pTGM2(V2), - producing lentiviruses containing pTGM2 gene by delivering psPAX2, pMD2.G, pTGM2(V2) plasmids to HEK293T cells in triplets, - when the mesenchymal stem cells (MSC) are at about 70% confluency, performing transduction process of the mesenchymal stem cells by adding lentiviruses onto the mesenchymal stem cells (MSC) in the culture dishes, - incubating the mesenchymal stem cells (MSC) in the CO 2 incubator, - at the end of the incubation, performing PBS washing and then seeding in a normal medium and proceeding with the usual cell culture, - incubating the transduced cells in a medium containing Blasticidin antibiotic and eliminating the cells that has not taken up the transgene from the medium, - proving that living cells have successfully acquired the gene, - preparing poly(1,4-butylene succinate)/ poly-L-lactide (PLLA) (PBSu/PLLA) polymer tissue scaffolds by using salt leaching technique, - seeding the cells onto the prepared PBSu/PLLA polymer tissue scaffolds, - after waiting for adhesion of the cells to the tissue scaffolds, adding medium to the culture dishes, - obtaining the nature identical cartilage tissue. <EOS>