Calibration device for determining the spatial orientation of a capillary held on a holder , the calibration device comprising - two detection beam paths (a, b), - a spatially resolving common detector , - at least one illumination source (2.1, 2.2) per detection beam path (a, b), - the holder for holding the capillary in the form of a cannula, a needle or a pipette tip, - an evaluation unit , wherein - the holder can be illuminated with illumination light from at least two different illumination angles by means of the illumination sources (2.1, 2.2), - the detection beam paths (a, b), along which in each case image data of the holder can be acquired sequentially at different capture angles by the common detector under each illumination at one of the illumination angles, are assigned to the common detector , - the detection beam paths (a, b) are brought together upstream of the common detector by means of beam-combining optical means or a suitable mirror and/or optical lenses to form a common detection beam path (c), and wherein - the evaluation unit is configured in such a way that, based on the image data acquired at the different capture angles, the presence and spatial orientation of a capillary held on the holder can be determined by evaluating the acquired image data. - two detection beam paths (a, b), - a spatially resolving common detector , - at least one illumination source (2.1, 2.2) per detection beam path (a, b), - the holder for holding the capillary in the form of a cannula, a needle or a pipette tip, - an evaluation unit , wherein - the holder can be illuminated with illumination light from at least two different illumination angles by means of the illumination sources (2.1, 2.2), - the detection beam paths (a, b), along which in each case image data of the holder can be acquired sequentially at different capture angles by the common detector under each illumination at one of the illumination angles, are assigned to the common detector , - the detection beam paths (a, b) are brought together upstream of the common detector by means of beam-combining optical means or a suitable mirror and/or optical lenses to form a common detection beam path (c), and wherein - the evaluation unit is configured in such a way that, based on the image data acquired at the different capture angles, the presence and spatial orientation of a capillary held on the holder can be determined by evaluating the acquired image data. - the holder can be illuminated with illumination light from at least two different illumination angles by means of the illumination sources (2.1, 2.2), - the detection beam paths (a, b), along which in each case image data of the holder can be acquired sequentially at different capture angles by the common detector under each illumination at one of the illumination angles, are assigned to the common detector , - the detection beam paths (a, b) are brought together upstream of the common detector by means of beam-combining optical means or a suitable mirror and/or optical lenses to form a common detection beam path (c), and wherein - the evaluation unit is configured in such a way that, based on the image data acquired at the different capture angles, the presence and spatial orientation of a capillary held on the holder can be determined by evaluating the acquired image data. <EOS>