Method for acquisition of fluorescence images and reflection images of an object comprising the steps of alternatingly illuminating the object with at least a first light and a second light, wherein the first light is spectrally shaped such that the first light has several spectral regions of high light intensity separated by spectral regions of low light intensity, wherein the ratio of the light intensity between regions of high intensity at shorter wavelength and regions of low light intensity of longer wavelength for the first light is ≥ 1 × 10 2 , wherein the second light is spectrally shaped such, that the second light has at least one spectral region of high intensity and at least one region of low intensity, that is of longer wavelength to the neighboring region of high light intensity, wherein the ratio of the light intensity between regions of high intensity at shorter wavelength and regions of low light intensity of longer wavelength for the second light is ≥ 1 × 10 2 , wherein the spectral regions of the first light with high intensity and the second light with high intensity at least partially do not overlap, and wherein the first light has at least one region of low light intensity that is of longer wavelength to the neighboring region of high light intensity, and recording at least a first image of the object and a second image of the object while illuminating the object with the first light, recording at least a first image of the object and a second image of the object while illuminating the object with the second light, wherein the light to be recorded as the first images is attenuated in at least one spectral region of high intensity of the second light with an attenuation ratio ≥ 1 × 10 2 , and wherein the light to be recorded as the second images is attenuated in at least one spectral region of high intensity of the first light with an attenuation ratio ≥ 1 × 10 2 . alternatingly illuminating the object with at least a first light and a second light, wherein the first light is spectrally shaped such that the first light has several spectral regions of high light intensity separated by spectral regions of low light intensity, wherein the ratio of the light intensity between regions of high intensity at shorter wavelength and regions of low light intensity of longer wavelength for the first light is ≥ 1 × 10 2 , wherein the second light is spectrally shaped such, that the second light has at least one spectral region of high intensity and at least one region of low intensity, that is of longer wavelength to the neighboring region of high light intensity, wherein the ratio of the light intensity between regions of high intensity at shorter wavelength and regions of low light intensity of longer wavelength for the second light is ≥ 1 × 10 2 , wherein the spectral regions of the first light with high intensity and the second light with high intensity at least partially do not overlap, and wherein the first light has at least one region of low light intensity that is of longer wavelength to the neighboring region of high light intensity, and recording at least a first image of the object and a second image of the object while illuminating the object with the first light, recording at least a first image of the object and a second image of the object while illuminating the object with the second light, wherein the light to be recorded as the first images is attenuated in at least one spectral region of high intensity of the second light with an attenuation ratio ≥ 1 × 10 2 , and wherein the light to be recorded as the second images is attenuated in at least one spectral region of high intensity of the first light with an attenuation ratio ≥ 1 × 10 2 . <EOS>