Method for calibrating an opto-electronic device for measuring the concentration of a biological compound, in particular bilirubin , the method comprising the steps of: a) performing a plurality of reflectance measurements for each reference strip of a plurality of reference strips (110-1, 110-2, ... 110-8) corresponding to predefined concentration values of the biological compound; b) calculating , for each reference strip, a respective value of a statistical reflectance indicator as a function of the plurality of reflectance measurements, generating a plurality of values of the statistical reflectance indicator corresponding to said plurality of reference strips, said plurality of values of the statistical reflectance indicator being selected from a plurality of mean reflectance values, or from a plurality of mode reflectance values, or from a plurality of median reflectance values; c) subdividing the plurality of values of the statistical reflectance indicator into at least two subsets (I, II, III), each one including consecutive values; d) interpolating the values of the statistical reflectance indicator of each subset with the corresponding predefined concentration value of the biological compound to generate an interpolation curve for each subset; e) calculating , for each pair of interpolation curves relating to two adjacent subsets (I, II), a threshold reflectance value (D, E) for which the difference of the reflectance values of the pair of interpolation curves is minimum; f) selecting, for each interpolation curve, a portion delimited at least in part by the respective threshold reflectance values, said portion being associated with the respective plurality of values of the statistical reflectance indicator; g) generating a calibration curve of the electronic device by combining the selected portions of the interpolation curves. a) performing a plurality of reflectance measurements for each reference strip of a plurality of reference strips (110-1, 110-2, ... 110-8) corresponding to predefined concentration values of the biological compound; b) calculating , for each reference strip, a respective value of a statistical reflectance indicator as a function of the plurality of reflectance measurements, generating a plurality of values of the statistical reflectance indicator corresponding to said plurality of reference strips, said plurality of values of the statistical reflectance indicator being selected from a plurality of mean reflectance values, or from a plurality of mode reflectance values, or from a plurality of median reflectance values; c) subdividing the plurality of values of the statistical reflectance indicator into at least two subsets (I, II, III), each one including consecutive values; d) interpolating the values of the statistical reflectance indicator of each subset with the corresponding predefined concentration value of the biological compound to generate an interpolation curve for each subset; e) calculating , for each pair of interpolation curves relating to two adjacent subsets (I, II), a threshold reflectance value (D, E) for which the difference of the reflectance values of the pair of interpolation curves is minimum; f) selecting, for each interpolation curve, a portion delimited at least in part by the respective threshold reflectance values, said portion being associated with the respective plurality of values of the statistical reflectance indicator; g) generating a calibration curve of the electronic device by combining the selected portions of the interpolation curves. <EOS>