Method for estimating fluorescent species in a fluorescent sample (C) capable of emitting a fluorescent signal (S), wherein said fluorescent signal (S) can be represented through phasors (F), comprising the following steps: a. generating a semi circumference , associable to said fluorescent signal (S) emitted by said fluorescent sample (C), on a Cartesian plane of coordinates (g, s) in the phasor space (F); b. defining a maximum number of primary components (N) in which said fluorescent signal (S) is to be divided; c. defining, for a first number of components N=2 and for the first harmonic H=1, a plurality of points (P) of coordinates (g , s ) on said semi circumference ; d. for each of said components (N), generating a plurality of possible polygons (K) whose vertices (V) coincide with a number of points of said plurality of points (P) equal to the number of components (N) defined in said step c.; e. calculating the coordinates (g',s') of the barycenter (B P ) of each polygon of said plurality of possible polygons (K); f. saving the vertices (V) and the barycenters (B P ) of all polygons of said plurality of possible polygons (K) generated in said step d.; g. repeating said steps d. -f. , for each harmonic following said first harmonic H = 1; h. repeating said step g., for each number of components (N) following said first number of components N=2; i. acquiring said fluorescent signal (S); j. defining the number of components (N) in which said fluorescent signal (S) is to be divided; k. calculating the barycenter (B) of said fluorescent signal (S) in coordinates (g, s); l . selecting the polygon of said plurality of polygons (K) whose barycenter (B P ) is at the minimum distance from said barycenter (B) calculated in said step k.; m. calculating the lifetimes (τ) of the (N) primary components from the coordinates (g, s) of the vertices (V) of said polygon selected in said step 1.; n. sending the values of the lifetimes (τ), calculated in said step m., to said interface (I). a. generating a semi circumference , associable to said fluorescent signal (S) emitted by said fluorescent sample (C), on a Cartesian plane of coordinates (g, s) in the phasor space (F); b. defining a maximum number of primary components (N) in which said fluorescent signal (S) is to be divided; c. defining, for a first number of components N=2 and for the first harmonic H=1, a plurality of points (P) of coordinates (g , s ) on said semi circumference ; d. for each of said components (N), generating a plurality of possible polygons (K) whose vertices (V) coincide with a number of points of said plurality of points (P) equal to the number of components (N) defined in said step c.; e. calculating the coordinates (g',s') of the barycenter (B P ) of each polygon of said plurality of possible polygons (K); f. saving the vertices (V) and the barycenters (B P ) of all polygons of said plurality of possible polygons (K) generated in said step d.; g. repeating said steps d. -f. , for each harmonic following said first harmonic H = 1; h. repeating said step g., for each number of components (N) following said first number of components N=2; i. acquiring said fluorescent signal (S); j. defining the number of components (N) in which said fluorescent signal (S) is to be divided; k. calculating the barycenter (B) of said fluorescent signal (S) in coordinates (g, s); l . selecting the polygon of said plurality of polygons (K) whose barycenter (B P ) is at the minimum distance from said barycenter (B) calculated in said step k.; m. calculating the lifetimes (τ) of the (N) primary components from the coordinates (g, s) of the vertices (V) of said polygon selected in said step 1.; n. sending the values of the lifetimes (τ), calculated in said step m., to said interface (I). <EOS>