Method for high throughput screening compounds by using a plurality of automated cell based assays assessing skeletal muscle cells contractility, morphology, and metabolism, in order to predict the efficacy of said compound on a panel of applications linked with muscle physiological and pathophysiological processes, comprising: (i) providing an in vitro culture of myotubes, wherein the in vitro myotubes culture is obtained by the following method: - providing a cell culture device allowing the culture of myoblasts or myotubes, - depositing skeletal muscle cells from a human donor or human group of donors, in good health or affected by a muscle related disorder, from primary cells, a cell line, an isogenic cell line or differentiated stem cells recapitulating a muscle disorder, on said culture device by using a method allowing the spatial control of cell culture, - culturing said cells during a determined incubation time so as to promote a spatially controlled myotube culture, (ii) adding at least one compound to said culture, (iii) after a determined incubation time of the myotubes with said compound, carrying out structural and functional readouts of the myotubes to determine the effect of said compound on the myotubes, said functional readouts comprising calcium release, and resulting myotube shortening in a high throughput assay through the quantification of the number of spots corresponding to the detachment and folding of at least one extremity of the myotubes, (iv) based on said determined effect, predicting the ability of said compound to improve or alter healthy muscle features, or to treat, rescue, or cure muscle disorders, said features or said disorders being linked with muscle contraction, muscle morphology or muscle metabolism. (i) providing an in vitro culture of myotubes, wherein the in vitro myotubes culture is obtained by the following method: - providing a cell culture device allowing the culture of myoblasts or myotubes, - depositing skeletal muscle cells from a human donor or human group of donors, in good health or affected by a muscle related disorder, from primary cells, a cell line, an isogenic cell line or differentiated stem cells recapitulating a muscle disorder, on said culture device by using a method allowing the spatial control of cell culture, - culturing said cells during a determined incubation time so as to promote a spatially controlled myotube culture, - providing a cell culture device allowing the culture of myoblasts or myotubes, - depositing skeletal muscle cells from a human donor or human group of donors, in good health or affected by a muscle related disorder, from primary cells, a cell line, an isogenic cell line or differentiated stem cells recapitulating a muscle disorder, on said culture device by using a method allowing the spatial control of cell culture, - culturing said cells during a determined incubation time so as to promote a spatially controlled myotube culture, (ii) adding at least one compound to said culture, (iii) after a determined incubation time of the myotubes with said compound, carrying out structural and functional readouts of the myotubes to determine the effect of said compound on the myotubes, said functional readouts comprising calcium release, and resulting myotube shortening in a high throughput assay through the quantification of the number of spots corresponding to the detachment and folding of at least one extremity of the myotubes, (iv) based on said determined effect, predicting the ability of said compound to improve or alter healthy muscle features, or to treat, rescue, or cure muscle disorders, said features or said disorders being linked with muscle contraction, muscle morphology or muscle metabolism. <EOS>