A method to select a particular voltage to cause a particular entrance blockage level of a nanopore by a protein comprising: flowing an ionic current through an open nanopore; measuring the ionic current through the open nanopore (I 0 ); applying one of a plurality of bias voltages across the open nanopore, causing a protein molecule to translocate through the open nanopore for each one of the plurality of the bias voltages; detecting a current blockage event by measuring the ionic current flow through the open nanopore while the protein molecule translocates through the open nanopore (I t ) for each one of the plurality of bias voltages; measuring duration of the current blockage event for each one of the plurality of bias voltages; classifying time-dependent current blockage signature for the plurality of bias voltages into permanent event signatures and transient event signatures; generating a protein dynamics landscape (PDL) for the transient event signatures for each of the plurality of bias voltages, the PDL comprising a collection of current blockage spectra for a set of increasing specified time values (τ trans ), wherein each current blockage spectrum comprises a histogram of the time spent at each current blockage level for the current blockage events that last less the specified time value (τ trans ), and wherein the PDL reveals peaks corresponding to discrete current blockage levels; identifying an entrance current blockage level based on the permanent event signatures, wherein the entrance current blockage level comprises an intermediate blockage level of variable duration prior to a transition to a permanent current blockage level; selecting one of the plurality of bias voltages that corresponds to a PDL for the transient event signatures where the PDL reveals peaks at current blockage levels that are substantially equal to the entrance current blockage level. flowing an ionic current through an open nanopore; measuring the ionic current through the open nanopore (I 0 ); applying one of a plurality of bias voltages across the open nanopore, causing a protein molecule to translocate through the open nanopore for each one of the plurality of the bias voltages; detecting a current blockage event by measuring the ionic current flow through the open nanopore while the protein molecule translocates through the open nanopore (I t ) for each one of the plurality of bias voltages; measuring duration of the current blockage event for each one of the plurality of bias voltages; classifying time-dependent current blockage signature for the plurality of bias voltages into permanent event signatures and transient event signatures; generating a protein dynamics landscape (PDL) for the transient event signatures for each of the plurality of bias voltages, the PDL comprising a collection of current blockage spectra for a set of increasing specified time values (τ trans ), wherein each current blockage spectrum comprises a histogram of the time spent at each current blockage level for the current blockage events that last less the specified time value (τ trans ), and wherein the PDL reveals peaks corresponding to discrete current blockage levels; identifying an entrance current blockage level based on the permanent event signatures, wherein the entrance current blockage level comprises an intermediate blockage level of variable duration prior to a transition to a permanent current blockage level; selecting one of the plurality of bias voltages that corresponds to a PDL for the transient event signatures where the PDL reveals peaks at current blockage levels that are substantially equal to the entrance current blockage level. <EOS>