A method, comprising: linearly moving a lift plate and a system plunger assembly carried by the lift plate toward a reagent cartridge plunger assembly of a reagent cartridge , the system plunger assembly including at least one system plunger , the reagent plunger assembly including at least one reagent cartridge plunger ; characterised by actuating the at least one reagent cartridge plunger a first predetermined distance to contact a gasket assembly of a flow cell assembly responsive to the at least one system plunger contacting the at least one reagent cartridge plunger , the flow cell assembly including a flow cell including at least one channel , a flow cell inlet , and a flow cell outlet , a first fluidic coupling fluidically coupled to the flow cell inlet and a second fluidic coupling fluidically coupled to the flow cell outlet , and the gasket assembly fluidically coupled to the first and second fluidic couplings and having an inlet gasket having a through bore and being fluidically coupled to the flow cell inlet via the first fluidic coupling , and an outlet gasket having a through bore and being fluidically coupled to the flow cell outlet via the second fluidic coupling ; and fluidically coupling the inlet gasket to a first reagent cartridge port of the reagent cartridge and fluidically coupling the outlet gasket to a second reagent cartridge port of the reagent cartridge responsive to the at least one system plunger actuating the at least one reagent cartridge plunger a second predetermined distance to allow fluid communication between the reagent cartridge ports and the flow cell via the flow cell inlet and the flow cell outlet . linearly moving a lift plate and a system plunger assembly carried by the lift plate toward a reagent cartridge plunger assembly of a reagent cartridge , the system plunger assembly including at least one system plunger , the reagent plunger assembly including at least one reagent cartridge plunger ; characterised by actuating the at least one reagent cartridge plunger a first predetermined distance to contact a gasket assembly of a flow cell assembly responsive to the at least one system plunger contacting the at least one reagent cartridge plunger , the flow cell assembly including a flow cell including at least one channel , a flow cell inlet , and a flow cell outlet , a first fluidic coupling fluidically coupled to the flow cell inlet and a second fluidic coupling fluidically coupled to the flow cell outlet , and the gasket assembly fluidically coupled to the first and second fluidic couplings and having an inlet gasket having a through bore and being fluidically coupled to the flow cell inlet via the first fluidic coupling , and an outlet gasket having a through bore and being fluidically coupled to the flow cell outlet via the second fluidic coupling ; and fluidically coupling the inlet gasket to a first reagent cartridge port of the reagent cartridge and fluidically coupling the outlet gasket to a second reagent cartridge port of the reagent cartridge responsive to the at least one system plunger actuating the at least one reagent cartridge plunger a second predetermined distance to allow fluid communication between the reagent cartridge ports and the flow cell via the flow cell inlet and the flow cell outlet . <EOS>