mhurhangee/patent-ind-claim-en huggingface dataset:
A method of extracting viral particles of a recombinant adeno-associated virus (AAV) or an adenovirus (AdV) from a sample comprising cells enclosing the viral particles, comprising: lysing the cells by the addition of an alkaline lysis solution, comprising a detergent and one or more reagents, to the sample such that the sample comprises 0.1% to 1% of the detergent and has a pH of 9.0 to 11.5 for AAV, or 9.0 to 10.5 for AdV, wherein the detergent is selected from the group consisting of deoxycholate, octyl thioglucoside, octyl glucoside, dodecyl maltoside, alkyl Sulfates, Polysorbate 20 (Tween-20), Tergitol-type NP-40 and the combinations thereof, and wherein the one or more reagents is selected from the group consisting of N-cyclohexyl-3-aminopropanesulfonic acid (CAPS), glycine/sodium, glycine/potassium, sodium carbonate, potassium carbonate, sodium phosphate, potassium-phosphate and combinations thereof; precipitating host cell proteins released from the lysed cells by (a) adding a salting solution to the sample such that the sample comprises 0.5 M to 2.0 M of a salt and has a pH of 4.0 to 7.0, wherein the salt is selected from the group consisting of sodium acetate, potassium acetate, sodium phosphate and combinations thereof and (b) removing the precipitated host cell proteins to obtain a supernatant comprising the viral particles; precipitating the viral particles from the supernatant with a precipitation solution, wherein upon addition of the precipitation solution to the sample, the sample comprises 5% to 12% of a polyethylene glycol (PEG); and further purifying the precipitated viral particles with chromatography. lysing the cells by the addition of an alkaline lysis solution, comprising a detergent and one or more reagents, to the sample such that the sample comprises 0.1% to 1% of the detergent and has a pH of 9.0 to 11.5 for AAV, or 9.0 to 10.5 for AdV, wherein the detergent is selected from the group consisting of deoxycholate, octyl thioglucoside, octyl glucoside, dodecyl maltoside, alkyl Sulfates, Polysorbate 20 (Tween-20), Tergitol-type NP-40 and the combinations thereof, and wherein the one or more reagents is selected from the group consisting of N-cyclohexyl-3-aminopropanesulfonic acid (CAPS), glycine/sodium, glycine/potassium, sodium carbonate, potassium carbonate, sodium phosphate, potassium-phosphate and combinations thereof; precipitating host cell proteins released from the lysed cells by (a) adding a salting solution to the sample such that the sample comprises 0.5 M to 2.0 M of a salt and has a pH of 4.0 to 7.0, wherein the salt is selected from the group consisting of sodium acetate, potassium acetate, sodium phosphate and combinations thereof and (b) removing the precipitated host cell proteins to obtain a supernatant comprising the viral particles; precipitating the viral particles from the supernatant with a precipitation solution, wherein upon addition of the precipitation solution to the sample, the sample comprises 5% to 12% of a polyethylene glycol (PEG); and further purifying the precipitated viral particles with chromatography.
A method for purification of a triacylglyceride oil, wherein the quantity of organic chlorinated precursors of monochloropropandiol esters (MCPDEs) is reduced in the purified triacylglyceride oil compared to the starting triacylglyceride oil, comprising the steps: (a) concentrating insoluble components in liquid triacylglyceride oil, for example microparticles, segregated droplets and sediments by (i). applying a centrifugational force on the triacylglyceride oil whilst maintaining the triacylglyceride oil above its melting temperature; and (ii). allowing the insoluble components to settle for at least 4 hours by gravitational force whilst maintaining the triacylglyceride oil above its melting temperature; (b) separating the triacylglyceride oil from the insoluble components; wherein step a(ii) is performed and then step a(i) is performed. (a) concentrating insoluble components in liquid triacylglyceride oil, for example microparticles, segregated droplets and sediments by (i). applying a centrifugational force on the triacylglyceride oil whilst maintaining the triacylglyceride oil above its melting temperature; and (ii). allowing the insoluble components to settle for at least 4 hours by gravitational force whilst maintaining the triacylglyceride oil above its melting temperature; (i). applying a centrifugational force on the triacylglyceride oil whilst maintaining the triacylglyceride oil above its melting temperature; and (ii). allowing the insoluble components to settle for at least 4 hours by gravitational force whilst maintaining the triacylglyceride oil above its melting temperature; (b) separating the triacylglyceride oil from the insoluble components;
A method for detecting an audio adversarial attack with respect to a voice command (VC) processed by an automatic speech recognition system (ASR), the method being implemented by a detection device connected to the automatic speech recognition system, wherein the method comprises: obtaining an audio signal (AS) associated with the voice command; performing a phonetic transcription of the audio signal, according to a phonetic transcription scheme, delivering a first character string (CS1); obtaining a transcript (T) resulting from the processing, by the automatic speech recognition system, of the audio signal; performing a phonetic transcription of the transcript, according to the phonetic transcription scheme, delivering a second character string (CS2); computing a similarity score (SS) between the first character string (CS1) and the second character string (CS2); and delivering a piece of data representative of a detection of an audio adversarial attack, as a function of a result of a comparison between the similarity score (SS) and a predetermined threshold. obtaining an audio signal (AS) associated with the voice command; performing a phonetic transcription of the audio signal, according to a phonetic transcription scheme, delivering a first character string (CS1); obtaining a transcript (T) resulting from the processing, by the automatic speech recognition system, of the audio signal; performing a phonetic transcription of the transcript, according to the phonetic transcription scheme, delivering a second character string (CS2); computing a similarity score (SS) between the first character string (CS1) and the second character string (CS2); and delivering a piece of data representative of a detection of an audio adversarial attack, as a function of a result of a comparison between the similarity score (SS) and a predetermined threshold.
A pulsed-light detection and ranging apparatus comprising: an optical detector arranged to generate, when in use, time-series data in response to an optical pulse incident thereupon; a processing resource arranged to support a pulse analyser , the pulse analyser being arranged to identify an inflection point of a pulse described by the time-series data; characterised in that the pulse analyser is further arranged to calculate a distance based upon the determined inflection point relative to a time axis associated with the time-series data; the pulse analyser is arranged to calculate a tangent line in respect of the pulse at the inflection point and to determine an intersection of the tangent line with the time axis, thereby providing a point of reference to determine a time of arrival of the pulse ; and the pulse analyser is further arranged to use the determined intersection of the tangent line with the time axis to calculate the distance. an optical detector arranged to generate, when in use, time-series data in response to an optical pulse incident thereupon; a processing resource arranged to support a pulse analyser , the pulse analyser being arranged to identify an inflection point of a pulse described by the time-series data; characterised in that the pulse analyser is further arranged to calculate a distance based upon the determined inflection point relative to a time axis associated with the time-series data; the pulse analyser is arranged to calculate a tangent line in respect of the pulse at the inflection point and to determine an intersection of the tangent line with the time axis, thereby providing a point of reference to determine a time of arrival of the pulse ; and the pulse analyser is further arranged to use the determined intersection of the tangent line with the time axis to calculate the distance.
A device for delivering nicotine to a subject, the device comprising a housing , the housing comprising: a) an inlet and an outlet in communication with each other and adapted so that a gaseous carrier may pass into the housing through the inlet , through the housing and out of the housing through the outlet , the device comprising from inlet to outlet : b) a first internal area in communication with the inlet , the first internal area comprising either a source of a compound for forming particles comprising nicotine , or a natural product nicotine source , c) a second internal area in communication with the first internal area, the second internal area comprising the source not selected for step b), and d) optionally, a third internal area in communication with the second internal area and the outlet , wherein the natural product nicotine source comprises tobacco and an added alkaline substance. a) an inlet and an outlet in communication with each other and adapted so that a gaseous carrier may pass into the housing through the inlet , through the housing and out of the housing through the outlet , the device comprising from inlet to outlet : b) a first internal area in communication with the inlet , the first internal area comprising either a source of a compound for forming particles comprising nicotine , or a natural product nicotine source , c) a second internal area in communication with the first internal area, the second internal area comprising the source not selected for step b), and d) optionally, a third internal area in communication with the second internal area and the outlet ,
A gas turbine engine component comprising: a heat exchange structure having an upstream end and a downstream end; a diffusing duct directly mounted to the upstream end; and a throttle member to control air flow through the heat exchange structure , wherein the throttle member has a non-circular cross section, wherein the diffusing duct includes a duct portion that increases in cross-sectional flow area from an upstream duct end to a downstream duct end , characterised in that : the diffusing duct comprises a first stage and a second stage downstream of the first stage , wherein the first stage comprises an axial duct portion of generally constant cross-sectional flow area, and the second stage comprises the duct portion that extends from the upstream duct end to the downstream duct end ; and the throttle member is mounted to the downstream end of the heat exchange structure . a heat exchange structure having an upstream end and a downstream end; a diffusing duct directly mounted to the upstream end; and a throttle member to control air flow through the heat exchange structure , wherein the throttle member has a non-circular cross section, wherein the diffusing duct includes a duct portion that increases in cross-sectional flow area from an upstream duct end to a downstream duct end , characterised in that : the diffusing duct comprises a first stage and a second stage downstream of the first stage , wherein the first stage comprises an axial duct portion of generally constant cross-sectional flow area, and the second stage comprises the duct portion that extends from the upstream duct end to the downstream duct end ; and the throttle member is mounted to the downstream end of the heat exchange structure . the diffusing duct comprises a first stage and a second stage downstream of the first stage , wherein the first stage comprises an axial duct portion of generally constant cross-sectional flow area, and the second stage comprises the duct portion that extends from the upstream duct end to the downstream duct end ; and the throttle member is mounted to the downstream end of the heat exchange structure .
A user equipment apparatus , comprising: means for receiving , while in active mode, a first probabilistic distribution factor relating to using a first access; means for determining , using a probabilistic procedure, based at least in part on the first probabilistic distribution factor, whether the user equipment apparatus is to use the first access, and means for selecting , based on a result of the determination, an access and for causing the apparatus to use to the selected access, the means for determining are configured to determine whether the user equipment apparatus is to use the first access responsive to receiving the first probabilistic distribution factor upon receipt of the first probabilistic distribution factor, characterized in that the means for determining are further configured such that, when using the probabilistic procedure, a subscription type of a subscriber of the user equipment affects how the first probabilistic distribution factor is used, in a manner that for premium-class subscribers distribution factors are ignored and always the access with a highest quality of service available is used and/or for budget-class subscribers distribution factors are employed skewed to cause attachment to an access with lower quality of service with a higher likelihood than with normal-class subscribers. means for receiving , while in active mode, a first probabilistic distribution factor relating to using a first access; means for determining , using a probabilistic procedure, based at least in part on the first probabilistic distribution factor, whether the user equipment apparatus is to use the first access, and means for selecting , based on a result of the determination, an access and for causing the apparatus to use to the selected access, the means for determining are configured to determine whether the user equipment apparatus is to use the first access responsive to receiving the first probabilistic distribution factor upon receipt of the first probabilistic distribution factor, characterized in that the means for determining are further configured such that, when using the probabilistic procedure, a subscription type of a subscriber of the user equipment affects how the first probabilistic distribution factor is used, in a manner that for premium-class subscribers distribution factors are ignored and always the access with a highest quality of service available is used and/or for budget-class subscribers distribution factors are employed skewed to cause attachment to an access with lower quality of service with a higher likelihood than with normal-class subscribers.
Decontamination device with a light source for a hollow object (OB) having a cavity and an outer surface (SE), comprising a light source (SL), an exposure substrate (SP) and a positioning member (OP), said light source (SL) comprising an oblong lamp having a radiating body (RX) protruding with respect to a cap (CL), said exposure substrate (SP) being provided for positioning and holding said hollow object (OB), said positioning member (OP) comprising axial positioning means for producing a relative movement of said light source (SL) with respect to said exposure substrate (SP) along the common axis thereof between a rest position and a working position so that in the working position the radiating body (RX) enters the cavity of the hollow object (OB), characterized in that the light source also comprises a reflector (RF) fixed on said cap (CL) so as to allow, in the working position, the illumination of at least a part of the outer surface (SE) of the object (OB) by reflection on said reflector (RF), of the light from a zone of the radiating body (RX) protruding with respect to the object (OB).
Guide device for a moveable furniture part, in particular a drawer, comprising a catch which is moveable along a guide track and which is pretensioned by an energy store and which is moveable at least between a parked position with a tensioned energy store and an end position with a less tensioned energy store, wherein the energy store can be coupled to an activator between the parked position and the end position in order to transmit a braking or acceleration force to the moveable furniture part, wherein the activator has a metal support part , characterized in that the metal support part , at least in an area of contact with the catch , is provided with a cover made of plastic, whereby the cover comprises a protrusion which is formed with a rounded surface and which engages in a U-shaped receptacle on the catch .
Optical guiding device for guiding a light beam (1.3) transporting an image, comprising: - a material forming a guide (1.5) equipped with two parallel planar faces (2.3, 2.4), the image-transporting light beam then being collimated; - an injection section (1.4) allowing the entry of the light beam into the guide (1.5), the beam (1.6) propagating through the guide via successive reflections from the two faces (2.3, 2.4) of the guide; - an extraction section (1.7) allowing the exit of the beam; in which the extraction section comprises a plurality of microstructures located on the surface of one of the two faces (3.2) of the guide (1.5), each microstructure comprising a reflective planar surface (3.4) that causes the exit, via the opposite face of the guide, of the light rays (3.5) of the beam that strike said reflective planar surface (3.4), said reflective planar surfaces (3.4) being parallel to one another, and characterized in that the extraction section comprises at least one transparent interstitial region that is formed from a planar section parallel to the surface of the guide and that is placed between said microstructures. - a material forming a guide (1.5) equipped with two parallel planar faces (2.3, 2.4), the image-transporting light beam then being collimated; - an injection section (1.4) allowing the entry of the light beam into the guide (1.5), the beam (1.6) propagating through the guide via successive reflections from the two faces (2.3, 2.4) of the guide; - an extraction section (1.7) allowing the exit of the beam;
A method for analyzing the expression of a survival motor neuron (SMN) protein nuclear body, comprising the steps of: labeling one or more surface antigen markers of blood-derived nucleated cells in a sample containing the nucleated cells with one or more label antibodies, wherein the one or more surface antigen markers comprise CD33; labeling SMN protein in the nucleated cells with a first fluorescent dye; labeling nuclei of the nucleated cells; selecting one cell population containing monocytes from a plurality of cell populations in which the nuclei and the SMN protein in the nucleated cells have been labeled and which have been classified based on the one or more surface antigen markers comprising CD33 labeled with the one or more label antibodies; and analyzing the expression of an SMN protein nuclear body of the selected cell population by measuring a fluorescence intensity emitted by the first fluorescent dye, wherein the step of selecting a cell population and the step of analyzing the expression of an SMN protein nuclear body are performed by imaging flow cytometry which uses an objective lens with a magnification of 40 times or more. labeling one or more surface antigen markers of blood-derived nucleated cells in a sample containing the nucleated cells with one or more label antibodies, wherein the one or more surface antigen markers comprise CD33; labeling SMN protein in the nucleated cells with a first fluorescent dye; labeling nuclei of the nucleated cells; selecting one cell population containing monocytes from a plurality of cell populations in which the nuclei and the SMN protein in the nucleated cells have been labeled and which have been classified based on the one or more surface antigen markers comprising CD33 labeled with the one or more label antibodies; and analyzing the expression of an SMN protein nuclear body of the selected cell population by measuring a fluorescence intensity emitted by the first fluorescent dye, wherein the step of selecting a cell population and the step of analyzing the expression of an SMN protein nuclear body are performed by imaging flow cytometry which uses an objective lens with a magnification of 40 times or more.
Method for detecting the alignment of at least one drill sleeve in a drill template , in particular relative to the jaw of a person, comprising the following steps: a. creating an impression element with a negative impression of the jaw structure of a person, b. creating an X-ray template from the impression element by introducing at least three X-ray visible reference bodies , spaced apart from one another, into the impression element , c. carrying out a first three-dimensional radiological detection, in which the jaw of the person, together with the X-ray template secured to the jaw with form-fit engagement, is detected, in particular by dental volume tomography or computer tomography, d. creating a drill template from the X-ray template by securing at least one drill sleeve in/on the X-ray template , in particular according to planning data for achieving a planned guide channel profile, e. inserting a reference object , having at least three spaced-apart X-ray visible reference bodies , into the at least one drill sleeve with form-fit engagement, f. carrying out a second three-dimensional radiological detection, in which only the drill template , located outside the jaw with its reference bodies , and the at least one reference object inserted therein, with its reference bodies , is detected. a. creating an impression element with a negative impression of the jaw structure of a person, b. creating an X-ray template from the impression element by introducing at least three X-ray visible reference bodies , spaced apart from one another, into the impression element , c. carrying out a first three-dimensional radiological detection, in which the jaw of the person, together with the X-ray template secured to the jaw with form-fit engagement, is detected, in particular by dental volume tomography or computer tomography, d. creating a drill template from the X-ray template by securing at least one drill sleeve in/on the X-ray template , in particular according to planning data for achieving a planned guide channel profile, e. inserting a reference object , having at least three spaced-apart X-ray visible reference bodies , into the at least one drill sleeve with form-fit engagement, f. carrying out a second three-dimensional radiological detection, in which only the drill template , located outside the jaw with its reference bodies , and the at least one reference object inserted therein, with its reference bodies , is detected.