A kit for making holes of controlled depth and different diameters on chondral and osteochondral surfaces, wherein it comprises an apparatus having: - a reamer member having a cutting head for removing tissue from said chondral and osteochondral surface (A), said member being mobile approaching the chondral and osteochondral surface (A) for arranging the head at said tissue; - guide means for guiding the reamer member that can be firmly associated with the chondral or osteochondral surface (A), said reamer member being slidable along said guide means ; said guide means comprising a pin partially insertable in the chondral and osteochondral surface (A); said reamer member being configured to be fitted onto the free end of the pin projecting from the chondral or osteochondral surface (A) for sliding axially along the longitudinal extension of the pin itself during the removal of the tissue from the chondral or osteochondral surface (A); and - mechanical means for controlling the depth of the hole interposed between the reamer member and the guide means for defining an end stop of the reamer member in the respective movement towards said chondral and osteochondral surface (A), characterised in that the kit further comprises: - an application element for applying the pin , having a body equipped with a through cavity for the hole of the pin ; and - an incision member , having at least one cutting tool for making an incision on the chondral or osteochondral reference surface (A) for positioning the cutting head ; and - a plurality of cylindrical inserts having different thicknesses (S) of the respective central walls corresponding to different hole depths, said cylindrical inserts defining the mechanical means for controlling the depth of the hole. - a reamer member having a cutting head for removing tissue from said chondral and osteochondral surface (A), said member being mobile approaching the chondral and osteochondral surface (A) for arranging the head at said tissue; - guide means for guiding the reamer member that can be firmly associated with the chondral or osteochondral surface (A), said reamer member being slidable along said guide means ; said guide means comprising a pin partially insertable in the chondral and osteochondral surface (A); said reamer member being configured to be fitted onto the free end of the pin projecting from the chondral or osteochondral surface (A) for sliding axially along the longitudinal extension of the pin itself during the removal of the tissue from the chondral or osteochondral surface (A); and - mechanical means for controlling the depth of the hole interposed between the reamer member and the guide means for defining an end stop of the reamer member in the respective movement towards said chondral and osteochondral surface (A), - an application element for applying the pin , having a body equipped with a through cavity for the hole of the pin ; and - an incision member , having at least one cutting tool for making an incision on the chondral or osteochondral reference surface (A) for positioning the cutting head ; and - a plurality of cylindrical inserts having different thicknesses (S) of the respective central walls corresponding to different hole depths, <EOS>