mhurhangee/patent-ind-claim-en huggingface dataset:
A method for generating a rat embryonic stem (ES) cell line, comprising: (a) culturing in vitro a first feeder cell layer that is not modified to express leukemia inhibitory factor (LIF) and a morula or a blastocyst stage rat embryo, wherein the zona pellucida of the morula or blastocyst stage rat embryo has been removed, and wherein the culture conditions maintain pluripotency of a rat ES cell and comprise a medium comprising about 50 U/mL to about 150 U/mL LIF, and a combination of inhibitors consisting of a MEK inhibitor and a GSK3 inhibitor; and (b) transferring an outgrowth of an amorphous undifferentiated mass of rat ES cells to an in vitro culture well comprising a second feeder cell layer that is not modified to express LIF; (c) culturing the outgrowth under conditions comprising the medium comprising about 50 U/mL to about 150 U/mL LIF, and a combination of inhibitors consisting of a MEK inhibitor and a GSK3 inhibitor, thereby maintaining pluripotency of the rat ES cells; and (d) modifying the rat ES cells to comprise a targeted genetic modification comprising at least one insertion of a heterologous polynucleotide into the genome of the rat ES cells, wherein the rat ES cells are capable of transmitting the targeted genetic modification through the germline. (a) culturing in vitro a first feeder cell layer that is not modified to express leukemia inhibitory factor (LIF) and a morula or a blastocyst stage rat embryo, wherein the zona pellucida of the morula or blastocyst stage rat embryo has been removed, and wherein the culture conditions maintain pluripotency of a rat ES cell and comprise a medium comprising about 50 U/mL to about 150 U/mL LIF, and a combination of inhibitors consisting of a MEK inhibitor and a GSK3 inhibitor; and (b) transferring an outgrowth of an amorphous undifferentiated mass of rat ES cells to an in vitro culture well comprising a second feeder cell layer that is not modified to express LIF; (c) culturing the outgrowth under conditions comprising the medium comprising about 50 U/mL to about 150 U/mL LIF, and a combination of inhibitors consisting of a MEK inhibitor and a GSK3 inhibitor, thereby maintaining pluripotency of the rat ES cells; and (d) modifying the rat ES cells to comprise a targeted genetic modification comprising at least one insertion of a heterologous polynucleotide into the genome of the rat ES cells, wherein the rat ES cells are capable of transmitting the targeted genetic modification through the germline.
Transport system for wave winding mats having a transport support , on which a wave winding mat can be placed and transported in a transport direction (P1), as well as having lateral wave winding mat guides which extend along the transport direction (P1), adjoin the transport support on both sides and prevent the wave winding mat from breaking out sideways during transport along the transport direction (P1), characterised in that at least a first of the wave winding mat guides has a through opening which extends in the transport direction along the wave winding mat guide and is permanently open or is to open at least in some sections, for passing through the wire end sections which are present on the wave winding mat .
A method for making a medical foam device, comprising: (a) solubilizing dehydrated extracellular matrix material obtained from a mammalian tissue in a solution comprising a pH less than 4.0 or a pH greater than 9.0; (b) foaming said acidified (pH< 4) or basic (pH> 9) solubilized extracellular matrix material in an industrial blender at speeds in the range of about 500 RPM to about 2500 RPM to form a foamy extracellular matrix material; (c) neutralizing said foamy extracellular matrix material in a solution to about pH 7; (d) mixing said foamy extracellular matrix material in the solution in step (c) to aid in neutralization of said foamy extracellular matrix material to a pH of about 7; (e) introducing said foamy extracellular matrix material of step (d) into a mold; (f) lyophilizing said neutralized molded foamy extracellular matrix material by pre-cooled lyophilizer shelves at a temperature range between 25°C to -40°C; followed by (g) stabilizing said material in step (f) by introducing ice crystals into said molded extracellular matrix material of step (f) at a temperature range between 0°C to -40°C at a rate of 0.01°C/min, to 1°C/min, change in temperature for periods of time ranging between 0 minutes to 240 minutes; followed by (h) sublimating said ice crystals introduced in step (g) at a vacuum pressure in the range of 100-120mmHg to produce a porous ECM medical foam device. (a) solubilizing dehydrated extracellular matrix material obtained from a mammalian tissue in a solution comprising a pH less than 4.0 or a pH greater than 9.0; (b) foaming said acidified (pH< 4) or basic (pH> 9) solubilized extracellular matrix material in an industrial blender at speeds in the range of about 500 RPM to about 2500 RPM to form a foamy extracellular matrix material; (c) neutralizing said foamy extracellular matrix material in a solution to about pH 7; (d) mixing said foamy extracellular matrix material in the solution in step (c) to aid in neutralization of said foamy extracellular matrix material to a pH of about 7; (e) introducing said foamy extracellular matrix material of step (d) into a mold; (f) lyophilizing said neutralized molded foamy extracellular matrix material by pre-cooled lyophilizer shelves at a temperature range between 25°C to -40°C; followed by (g) stabilizing said material in step (f) by introducing ice crystals into said molded extracellular matrix material of step (f) at a temperature range between 0°C to -40°C at a rate of 0.01°C/min, to 1°C/min, change in temperature for periods of time ranging between 0 minutes to 240 minutes; followed by (h) sublimating said ice crystals introduced in step (g) at a vacuum pressure in the range of 100-120mmHg to produce a porous ECM medical foam device.
A power tool comprising: an electric brushless direct current (BLDC) motor having a rotor and a stator defining a plurality of phases (PV, PU, PW); a first power supply and a second power supply , both comprising direct current (DC) power supplies; a power unit including: a first switch circuit connected electrically between the first power supply via a first DC bus line and the motor , and a second switch circuit connected electrically between the second power supply via a second DC bus line and the motor , to effectively provide a parallel connection between the first power supply and the second power supply ; and a controller configured to control a switching operation of the first switch circuit and the second switch circuit to regulate a supply of power from the first power supply and the second power supply (372 to the motor ; wherein the stator comprises a plurality of windings associated with the plurality of phases (PV, PU, PW), the first switch circuit being electrically connected to a first set of stator windings of the plurality of windings, and the second switch circuit is electrically connected to a second set of stator windings of the plurality of windings; characterized in that there is further provided a battery selector switch disposed between the first power supply and the second switch circuit to selectively couple the first power supply to the second switch circuit when the second power supply is not present. an electric brushless direct current (BLDC) motor having a rotor and a stator defining a plurality of phases (PV, PU, PW); a first power supply and a second power supply , both comprising direct current (DC) power supplies; a power unit including: a first switch circuit connected electrically between the first power supply via a first DC bus line and the motor , and a second switch circuit connected electrically between the second power supply via a second DC bus line and the motor , to effectively provide a parallel connection between the first power supply and the second power supply ; and a controller configured to control a switching operation of the first switch circuit and the second switch circuit to regulate a supply of power from the first power supply and the second power supply (372 to the motor ; wherein the stator comprises a plurality of windings associated with the plurality of phases (PV, PU, PW), the first switch circuit being electrically connected to a first set of stator windings of the plurality of windings, and the second switch circuit is electrically connected to a second set of stator windings of the plurality of windings; characterized in that there is further provided a battery selector switch disposed between the first power supply and the second switch circuit to selectively couple the first power supply to the second switch circuit when the second power supply is not present.
A method for determining a synchronization accuracy of a time synchronization of a first communication unit , the method comprising: sending a time request via a communication channel at a corresponding transmission time (TS1) from the first communication unit to a second communication unit ; receiving a time response , wherein the time response comprises a synchronization time specification of the second communication unit at a corresponding reception time (TS2) at the first communication unit ; determining the synchronization accuracy based on the transmission time (TS1) and the reception time (TS2); and using the synchronization time specification of the second communication unit for executing a function of the first communication unit depending on the synchronization accuracy, wherein a wired network is used as the communication channel and comprises at least one component having a functional reliability level that lies below a functional reliability level required by the function of the first communication unit so that it can be determined whether a required predetermined synchronization accuracy is present during time synchronization via the wired network, characterized in that a synchronization error determined based on a round trip time between the transmission time (TS1) and the reception time (TS2) is taken into account for the time synchronization depending on the function to be executed. sending a time request via a communication channel at a corresponding transmission time (TS1) from the first communication unit to a second communication unit ; receiving a time response , wherein the time response comprises a synchronization time specification of the second communication unit at a corresponding reception time (TS2) at the first communication unit ; determining the synchronization accuracy based on the transmission time (TS1) and the reception time (TS2); and using the synchronization time specification of the second communication unit for executing a function of the first communication unit depending on the synchronization accuracy, wherein a wired network is used as the communication channel and comprises at least one component having a functional reliability level that lies below a functional reliability level required by the function of the first communication unit so that it can be determined whether a required predetermined synchronization accuracy is present during time synchronization via the wired network, characterized in that a synchronization error determined based on a round trip time between the transmission time (TS1) and the reception time (TS2) is taken into account for the time synchronization depending on the function to be executed.
A method of consolidating a wound body, the method comprising: onto an elongated member , winding (S1) a film of a thermoplastic material for forming a wound body comprising a plurality of wound layers of the film around said elongated member; and during said winding, applying (S2) a high-frequency, HF, electrical field formed between a first and a second electrode at a nip where a web of the film is pressed onto a previously wound layer of the body, to heat said body at said nip; wherein the second electrode is grounded and comprised in the elongated member ; wherein the first electrode is comprised in a roll (3/4/5) forming the nip ; and wherein an adhesive is applied between the layers of the film, and wherein the body at the nip is heated to a temperature which is above a softening or melting temperature of the adhesive, but below the melting temperature, Tm, of the thermoplastic material. onto an elongated member , winding (S1) a film of a thermoplastic material for forming a wound body comprising a plurality of wound layers of the film around said elongated member; and during said winding, applying (S2) a high-frequency, HF, electrical field formed between a first and a second electrode at a nip where a web of the film is pressed onto a previously wound layer of the body, to heat said body at said nip; wherein the second electrode is grounded and comprised in the elongated member ; wherein the first electrode is comprised in a roll (3/4/5) forming the nip ; and wherein an adhesive is applied between the layers of the film, and wherein the body at the nip is heated to a temperature which is above a softening or melting temperature of the adhesive, but below the melting temperature, Tm, of the thermoplastic material.
An electromagnetic device comprising: - a conductive coil comprising a first and a second ends, and extending along a main axis XX', - a main magnet , having a symmetry of revolution about an axis YY' perpendicular to the main axis XX', held by holding means in an internal volume V formed by the conductive coil , the holding means allowing rotational movement of said main magnet about the axis YY' between two positions of stable equilibrium called, respectively, first position of equilibrium and second position of equilibrium, - a first and a second actuator magnets disposed in a slide, respectively, facing the first end and the second end , each having a magnetic polarity in a plane perpendicular to the axis YY', the first and second actuator magnets being arranged to be driven in translation simultaneously in the same way and parallel to the main axis XX' once a force is exerted on either of the first and second magnets, the sliding of the first and second magnets according to either of the ways defined by the direction of the main axis XX' being adapted to force the main magnet to adopt, respectively, the first position of equilibrium or the second position of equilibrium. - a conductive coil comprising a first and a second ends, and extending along a main axis XX', - a main magnet , having a symmetry of revolution about an axis YY' perpendicular to the main axis XX', held by holding means in an internal volume V formed by the conductive coil , the holding means allowing rotational movement of said main magnet about the axis YY' between two positions of stable equilibrium called, respectively, first position of equilibrium and second position of equilibrium, - a first and a second actuator magnets disposed in a slide, respectively, facing the first end and the second end , each having a magnetic polarity in a plane perpendicular to the axis YY', the first and second actuator magnets being arranged to be driven in translation simultaneously in the same way and parallel to the main axis XX' once a force is exerted on either of the first and second magnets, the sliding of the first and second magnets according to either of the ways defined by the direction of the main axis XX' being adapted to force the main magnet to adopt, respectively, the first position of equilibrium or the second position of equilibrium.
Breast pump device , comprising: - a fluid pressure arrangement configured to interact with a breast from which milk is to be extracted and to realize a pressure cycle at a position where the fluid pressure arrangement is to face the breast, the pressure cycle involving increase and decrease of a level of underpressure by the fluid pressure arrangement , - a controller configured to execute an operation software program for controlling operation of the breast pump device , the operation software program including instructions to cause the fluid pressure arrangement to realize the pressure cycle, the breast pump device being characterized in - a monitoring arrangement configured to monitor functioning of the operation software program and to cause release of underpressure in case of detection of malfunctioning of the operation software program related to sustained underpressure. - a fluid pressure arrangement configured to interact with a breast from which milk is to be extracted and to realize a pressure cycle at a position where the fluid pressure arrangement is to face the breast, the pressure cycle involving increase and decrease of a level of underpressure by the fluid pressure arrangement , - a controller configured to execute an operation software program for controlling operation of the breast pump device , the operation software program including instructions to cause the fluid pressure arrangement to realize the pressure cycle, the breast pump device being characterized in - a monitoring arrangement configured to monitor functioning of the operation software program and to cause release of underpressure in case of detection of malfunctioning of the operation software program related to sustained underpressure.
A stable liquid pharmaceutical formulation of a recombinant von Willebrand Factor (rVWF) comprising: (a) a rVWF; (b) a buffering agent; (c) one or more salts; (d) a stabilizing agent; and (e) a optionally a surfactant; wherein the rVWF is capable of causing agglutination of stabilized platelets in the presence of ristocetin; wherein said rVWF comprises a polypeptide selected from the group consisting of: a) the amino acid sequence set out in SEQ ID NO: 3; b) a biologically active analog, fragment or variant of a); c) a polypeptide encoded by the polynucleotide set out in SEQ ID NO: 1; d) a biologically active analog, fragment or variant of c); and e) a polypeptide encoded by a polynucleotide that hybridizes to the polynucleotide set out in SEQ ID NO: 1 under moderately stringent hybridization conditions; wherein said buffer is comprised of a pH buffering agent in a range of 0.1 mM to 500 mM and wherein the pH is in a range of 2.0 to 12.0; wherein said salt is at a concentration of 1 to 500 mM; wherein said stabilizing agent is at a concentration of 0.1 to 1000 mM and is selected from the group consisting of mannitol, lactose, sorbitol, xylitol, sucrose, trehalose, mannose, maltose, lactose, glucose, raffinose, cellobiose, gentiobiose, isomaltose, arabinose, glucosamine, fructose, and combinations of these stabilizing agents; and wherein said surfactant is at a concentration of 0.01 g/L to 0.5 g/L. a) the amino acid sequence set out in SEQ ID NO: 3; b) a biologically active analog, fragment or variant of a); c) a polypeptide encoded by the polynucleotide set out in SEQ ID NO: 1; d) a biologically active analog, fragment or variant of c); and e) a polypeptide encoded by a polynucleotide that hybridizes to the polynucleotide set out in SEQ ID NO: 1 under moderately stringent hybridization conditions;
A method for characterizing extracellular vesicles at an individual level, the method comprising - providing an obtained sample comprising extracellular vesicles to be characterized, - functionalizing the extracellular vesicles with plasmonic material by providing a coating of plasmonic material on the extracellular vesicles or applying at least one of plasmonic nanoparticles to the membrane, in a phospholipid layer or in the lumen of the extracellular vesicles to obtain functionalized individual extracellular vesicles, and - irradiating the functionalized individual extracellular vesicles with a laser beam and detecting a surface enhanced Raman spectroscopy signal from said functionalized individual extracellular vesicles. - providing an obtained sample comprising extracellular vesicles to be characterized, - functionalizing the extracellular vesicles with plasmonic material by providing a coating of plasmonic material on the extracellular vesicles or applying at least one of plasmonic nanoparticles to the membrane, in a phospholipid layer or in the lumen of the extracellular vesicles to obtain functionalized individual extracellular vesicles, and - irradiating the functionalized individual extracellular vesicles with a laser beam and detecting a surface enhanced Raman spectroscopy signal from said functionalized individual extracellular vesicles.
Device for controlling the quality of a textile seam for a sewing machine comprising: a rear presser foot with compensation comprising two forks for pressing the workpiece being sewn, a fork for each side of the seam, after it passes through the needle seam area, wherein said forks are vertically and independently oscillating; characterized by further comprising a sensor configured to detect the vertical displacement of a first fork; a signal processor configured to detect if the vertical displacement detected by the sensor when the first fork is pressing the workpiece is above a predetermined value of excessive thickness. a rear presser foot with compensation comprising two forks for pressing the workpiece being sewn, a fork for each side of the seam, after it passes through the needle seam area, wherein said forks are vertically and independently oscillating; characterized by further comprising a sensor configured to detect the vertical displacement of a first fork; a signal processor configured to detect if the vertical displacement detected by the sensor when the first fork is pressing the workpiece is above a predetermined value of excessive thickness.
A pressure-sensitive adhesive polymer (PSA) polymerized from monomers including a skin-mimicking monomer having a skin-mimicking polar group, wherein the skin-mimicking monomer is selected from the group consisting of monomers having a glucosyl group, monomers having two or more hydroxyl groups and an n-(hydroxymethyl) group, wherein n is an integer from 2-6, and combinations of any two or more thereof.
An agricultural implement comprising an agricultural toolbar and multiple row units mounted to the agricultural toolbar , the agricultural implement comprising a respective downforce controller for each row unit , each downforce controller comprising a double-acting hydraulic cylinder having a first chamber and a second chamber , said cylinder coupled to the agricultural row unit and the agricultural toolbar for transmitting a net downforce between said agricultural toolbar and said agricultural row unit, a first pressure in said first chamber and a second pressure in said second chamber having counteracting effects on said net downforce; characterised in that a manifold is coupled to and supported by said cylinder , said manifold being in fluid communication with said first chamber ; and a pressure control valve is coupled to and supported by said manifold , said pressure control valve being in fluid communication with said manifold and with said first chamber , wherein said pressure control valve is configured to maintain any one of a continuous range of pressures in said first chamber , and wherein said manifold includes a counteracting pressure control passage in fluid communication with said second chamber .