mhurhangee/patent-ind-claim-en huggingface dataset:
A method of enriching a region of interest, wherein the region of interest is double stranded DNA, and wherein the method comprises: (a) contacting a sample comprising double stranded DNA sequences with a plurality of Cas9 nickase-gRNA complexes, wherein the gRNAs are complementary to targeted sites of interest on one side of the region of interest in a subset of the double stranded DNA sequences, and wherein Cas9 nickase-gRNA complexes are targeted to proximal sites on opposite strands of the subset of the double stranded DNA sequences, thereby generating a plurality of nicked DNA at sites on one side of the region of interest; (b) heating the sample to 65°C to cause nicks in close proximity on opposite strands to generate double stranded breaks; (c) contacting the double stranded breaks with a thermostable ligase and ligating adapter sequences at these sites only; and (d) repeating steps a-c to place a second adapter on the other side of the region of interest, thus allowing enrichment of the region of interest, wherein the gRNAs are complementary to targeted sites of interest on the other side of the region of interest in a subset of the double stranded DNA sequences. (a) contacting a sample comprising double stranded DNA sequences with a plurality of Cas9 nickase-gRNA complexes, wherein the gRNAs are complementary to targeted sites of interest on one side of the region of interest in a subset of the double stranded DNA sequences, and wherein Cas9 nickase-gRNA complexes are targeted to proximal sites on opposite strands of the subset of the double stranded DNA sequences, thereby generating a plurality of nicked DNA at sites on one side of the region of interest; (b) heating the sample to 65°C to cause nicks in close proximity on opposite strands to generate double stranded breaks; (c) contacting the double stranded breaks with a thermostable ligase and ligating adapter sequences at these sites only; and (d) repeating steps a-c to place a second adapter on the other side of the region of interest, thus allowing enrichment of the region of interest, wherein the gRNAs are complementary to targeted sites of interest on the other side of the region of interest in a subset of the double stranded DNA sequences.
A shock absorber comprising: a main damper cylinder having a variable volume portion with a fluid therein; a first reservoir and a second reservoir and a first fluid flow path between the variable volume portion and the first reservoir ; and a second fluid flow path between the first and second reservoirs; characterized in that said first and second fluid flow paths, and said first and second reservoirs, define a first fluid transfer path from the main damper cylinder to the second reservoir that includes the first reservoir and the second reservoir in series, wherein in use fluid flows along said first fluid transfer path both in extension and compression of the shock absorber and whereby pressure in the first and second reservoirs remain relatively equalized during operation; at least one valve for permitting selective communication between the main damper cylinder and the first and second reservoirs; and further including a first sequential operational mode utilizing said first fluid transfer path from the main damper cylinder , to the at least one valve , to the first reservoir and thereafter, to the second reservoir . a main damper cylinder having a variable volume portion with a fluid therein; a first reservoir and a second reservoir and a first fluid flow path between the variable volume portion and the first reservoir ; and a second fluid flow path between the first and second reservoirs; characterized in that said first and second fluid flow paths, and said first and second reservoirs, define a first fluid transfer path from the main damper cylinder to the second reservoir that includes the first reservoir and the second reservoir in series, wherein in use fluid flows along said first fluid transfer path both in extension and compression of the shock absorber and whereby pressure in the first and second reservoirs remain relatively equalized during operation; at least one valve for permitting selective communication between the main damper cylinder and the first and second reservoirs; and further including a first sequential operational mode utilizing said first fluid transfer path from the main damper cylinder , to the at least one valve , to the first reservoir and thereafter, to the second reservoir .
Tensioning device for tensioning traction means of a traction drive, comprising: a base member ; a first tensioning arm , pivotably supported on the base member and carrying a first tensioning roller ; a second tensioning arm , pivotably supported on the base member and carrying a second tensioning roller ; a spring arrangement with a first spring and a second spring , arranged to be effective between the first and the second tensioning arm ; wherein the two tensioning arms , when being pivoted relative to each other within a first tensioning angle range, are force-loaded against each other by the first spring , characterised in that the second spring within the first tensioning angle range is force-free relative to at least one of the two tensioning arms ; and that the two tensioning arms , when being pivoted relative to each other within a second tensioning angle range comprising larger tensioning angles than the first tensioning angle range, are force-loaded against each other by the first spring and additionally by the second spring . a base member ; a first tensioning arm , pivotably supported on the base member and carrying a first tensioning roller ; a second tensioning arm , pivotably supported on the base member and carrying a second tensioning roller ; a spring arrangement with a first spring and a second spring , arranged to be effective between the first and the second tensioning arm ; wherein the two tensioning arms , when being pivoted relative to each other within a first tensioning angle range, are force-loaded against each other by the first spring , characterised in that the second spring within the first tensioning angle range is force-free relative to at least one of the two tensioning arms ; and that the two tensioning arms , when being pivoted relative to each other within a second tensioning angle range comprising larger tensioning angles than the first tensioning angle range, are force-loaded against each other by the first spring and additionally by the second spring .
A method of forming an infrared detector (10a-10b) comprising the steps of: - definition of an optical window in a first substrate, called cover substrate ; - anodic bonding of a glass spacer or a glass insert bonded to a silicon spacer onto a lower surface of said cover substrate at the periphery of said optical window to form the lateral walls of a package; - transfer of said cover substrate provided with said spacer onto a second substrate, called base substrate , having at least one microbolometer suspended thereon; and - hermetic bonding of said spacer onto said base substrate to form a hermetic package around said at least one microbolometer ; wherein the step of definition of the optical window comprises forming a multilayer interference filter or a periodic diffraction grating on an upper surface of said optical window , and a periodic diffraction grating on the lower surface of said optical window ; the multilayer interference filter or the periodic diffraction grating of the upper surface being configured to attenuate the incident radiation in a first wavelength interval in the range from 2 to x micrometers; the periodic diffraction grating of the lower surface being configured to attenuate the incident radiation in a second wavelength interval in the range from y to 8 micrometers; with x in the range from 2 to 8 micrometers and y smaller than or equal to x. - definition of an optical window in a first substrate, called cover substrate ; - anodic bonding of a glass spacer or a glass insert bonded to a silicon spacer onto a lower surface of said cover substrate at the periphery of said optical window to form the lateral walls of a package; - transfer of said cover substrate provided with said spacer onto a second substrate, called base substrate , having at least one microbolometer suspended thereon; and - hermetic bonding of said spacer onto said base substrate to form a hermetic package around said at least one microbolometer ; wherein the step of definition of the optical window comprises forming a multilayer interference filter or a periodic diffraction grating on an upper surface of said optical window , and a periodic diffraction grating on the lower surface of said optical window ; the multilayer interference filter or the periodic diffraction grating of the upper surface being configured to attenuate the incident radiation in a first wavelength interval in the range from 2 to x micrometers; the periodic diffraction grating of the lower surface being configured to attenuate the incident radiation in a second wavelength interval in the range from y to 8 micrometers; with x in the range from 2 to 8 micrometers and y smaller than or equal to x.
A method for wireless communication, the method comprising: receiving, at an access point during a first transmit opportunity, TX_OP, used by a first device and a second device , at least a first data packet from the first device and a second data packet from the second device, the first data packet including a first data fragment and the second data packet including a second data fragment ; generating, at the access point, a block acknowledgement frame (700; 900) including at least a first block acknowledgement bitmap (706; 906) and a second block acknowledgement bitmap, wherein the first block acknowledgement bitmap indicates at least the first data fragment received from the first device, and wherein the second block acknowledgement bitmap indicates at least the second data fragment received from the second device and wherein the block acknowledgement frame includes one or more bits indicating whether the bitmaps relate to one or more data fragments or to all data fragments from the respective device ; and transmitting the block acknowledgement frame to the first device. receiving, at an access point during a first transmit opportunity, TX_OP, used by a first device and a second device , at least a first data packet from the first device and a second data packet from the second device, the first data packet including a first data fragment and the second data packet including a second data fragment ; generating, at the access point, a block acknowledgement frame (700; 900) including at least a first block acknowledgement bitmap (706; 906) and a second block acknowledgement bitmap, wherein the first block acknowledgement bitmap indicates at least the first data fragment received from the first device, and wherein the second block acknowledgement bitmap indicates at least the second data fragment received from the second device and wherein the block acknowledgement frame includes one or more bits indicating whether the bitmaps relate to one or more data fragments or to all data fragments from the respective device ; and transmitting the block acknowledgement frame to the first device.
A cable tray assembly for installing one or more cable harnesses in a rail vehicle body , comprising a main tray having at least a first cable channel extending in a longitudinal direction of the cable tray assembly and a second cable channel at a distance from and parallel to the first cable channel , wherein the first cable channel has a flat bottom and at least one side wall extending parallel to the longitudinal axis, the second cable channel has a flat bottom and at least one side wall extending parallel to the longitudinal axis, and the cable tray assembly further comprises an auxiliary tray provided with an auxiliary tray plate for holding one or more pieces of electrical equipment , the auxiliary tray plate facing the main tray at a distance from the main tray and legs extending from the auxiliary tray plate towards the main tray , the legs being attached to attachment portions of the main tray in a releasable manner, characterised in that the main tray has plastically deformable bridges connecting the first channel and second channel , the bridges being bent, so that the flat bottom of the first channel and the flat bottom of the second channel are in different planes.
A biopsy needle device comprising: - a biopsy needle attachment mechanism arranged to mechanically couple a biopsy needle to the biopsy needle device ; - an actuator mechanism comprising a transducer configured to interconnect electrical signals at one port to mechanical motion at another port, the actuator mechanism configured to transmit flexural vibration to the biopsy needle when the biopsy needle is coupled to the biopsy needle device , characterized by - a sensor device configured to measure a power of the flexural vibration transmitted to the biopsy needle via the transducer and a reflected power of flexural vibration received by the biopsy needle device from the biopsy needle , and - circuitry configured to determine a difference between the power of the flexural vibration transmitted to the biopsy needle and the reflected power of flexural vibration received by the biopsy needle device from the biopsy needle . - a biopsy needle attachment mechanism arranged to mechanically couple a biopsy needle to the biopsy needle device ; - an actuator mechanism comprising a transducer configured to interconnect electrical signals at one port to mechanical motion at another port, the actuator mechanism configured to transmit flexural vibration to the biopsy needle when the biopsy needle is coupled to the biopsy needle device , characterized by - a sensor device configured to measure a power of the flexural vibration transmitted to the biopsy needle via the transducer and a reflected power of flexural vibration received by the biopsy needle device from the biopsy needle , and - circuitry configured to determine a difference between the power of the flexural vibration transmitted to the biopsy needle and the reflected power of flexural vibration received by the biopsy needle device from the biopsy needle .
A gas turbine engine comprising a component, the component comprising: an airfoil having a leading edge area ; a first circuit to cool a first section of the leading edge area ; and a second circuit to cool a second section of the leading edge area , wherein the first circuit is separate and distinct from the second circuit within the airfoil , wherein flow across the airfoil has an incidence angle relative to the airfoil , characterised in that : the leading edge area represents an area of the airfoil traversed by a maximum stagnation point of flow across the airfoil through one-hundred-eighty degrees of incident angle shift. an airfoil having a leading edge area ; a first circuit to cool a first section of the leading edge area ; and a second circuit to cool a second section of the leading edge area , wherein the first circuit is separate and distinct from the second circuit within the airfoil , wherein flow across the airfoil has an incidence angle relative to the airfoil , characterised in that : the leading edge area represents an area of the airfoil traversed by a maximum stagnation point of flow across the airfoil through one-hundred-eighty degrees of incident angle shift.
A resin-made syringe comprising a syringe body made of polyolefin and a printing layer printed on at least a part of an outer surface of the syringe body , characterised in that the printing layer has components including chlorinated polypropylene having a degree of chlorination of 15 to 30%, a colorant, and a filler, the outer surface on which the printing layer is printed has a plurality of recessed portions , and each of the plurality of recessed portions has a depth (d1) of 50 nm or more and less than 1 μm and a width (d2) of 50 nm or more and less than 1 μm and is filled with at least one kind of the components of the printing layer . the printing layer has components including chlorinated polypropylene having a degree of chlorination of 15 to 30%, a colorant, and a filler, the outer surface on which the printing layer is printed has a plurality of recessed portions , and each of the plurality of recessed portions has a depth (d1) of 50 nm or more and less than 1 μm and a width (d2) of 50 nm or more and less than 1 μm and is filled with at least one kind of the components of the printing layer .
A crystalline form of -8-hydroxy-7,9-dioxo-N-(2,4,6-trifluorobenzyl)-2,3,4,5,7,9,13,13a-octahydro-2,5-methanopyrido[1',2':4,5]pyrazino[2,1-b] oxazepine-10-carboxamide, wherein the crystalline form is Form III, characterized by an x-ray powder diffraction (XRPD) pattern having peaks at 9.6°, 14.0°, and 18.5° 2-θ ± 0.2° 2-θ.
A flexographic printing plate precursor having a photosensitive layer , wherein the photosensitive layer comprises A) a water-dispersed latex, B) a millable rubber, C) a surfactant, D) photopolymerizable compounds, and E) a photopolymerization initiator, and the D) comprises the following d1) to d3), and d1) is a compound represented by the following formula , d1) a photopolymerizable compound of a chain structure having no cyclic structure, d2) a monofunctional photopolymerizable compound having a cyclic structure, and d3) a bifunctional photopolymerizable compound having a cyclic structure, in the formula , R 1 represents a hydrogen group or a methyl group, R 2 represents a hydrogen group or a methyl group, and R 3 represents a linear or branched hydrocarbon group having a carbon number of 4 to 12, wherein a total amount of the d1) to d3) is within a range of 7.5 to 19 mass% with respect to an entire amount of the photosensitive layer, a content of the d1) is within a range of 38 to 67 mass% with respect to the total amount of the d1) to d3), a content of the d2) is within a range of 12 to 46 mass% with respect to the total amount of the d1) to d3), and a content of the d3) is within a range of 9 to 46 mass% with respect to the total amount of the d1) to d3). the photosensitive layer comprises A) a water-dispersed latex, B) a millable rubber, C) a surfactant, D) photopolymerizable compounds, and E) a photopolymerization initiator, and the D) comprises the following d1) to d3), and d1) is a compound represented by the following formula , d1) a photopolymerizable compound of a chain structure having no cyclic structure, d2) a monofunctional photopolymerizable compound having a cyclic structure, and d3) a bifunctional photopolymerizable compound having a cyclic structure, in the formula , R 1 represents a hydrogen group or a methyl group, R 2 represents a hydrogen group or a methyl group, and R 3 represents a linear or branched hydrocarbon group having a carbon number of 4 to 12, wherein a total amount of the d1) to d3) is within a range of 7.5 to 19 mass% with respect to an entire amount of the photosensitive layer, a content of the d1) is within a range of 38 to 67 mass% with respect to the total amount of the d1) to d3), a content of the d2) is within a range of 12 to 46 mass% with respect to the total amount of the d1) to d3), and a content of the d3) is within a range of 9 to 46 mass% with respect to the total amount of the d1) to d3).
A gas turbine plant transition duct comprising: a tubular body having an upstream end configured to be coupled to a combustor of a gas turbine plant and a downstream end configured to be coupled to a turbine of the gas turbine plant ; the tubular body comprising: an outer wall ; an inner wall defining a transition channel ; at least one first convective cooling channel arranged between the inner wall and the outer wall ; the at least one first convective cooling channel having at least one first inlet (46; 50) made in the outer wall between the upstream end and the downstream end in communication with a source of cooling fluid ; the at least one first convective cooling channel comprising at least one inlet portion (43; 48) comprising the at least one first inlet (46; 50), a main portion and at least one bent portion (45; 49) arranged between the at least one inlet portion (43; 48) and the main portion ; wherein in the at least one inlet portion (43; 48) the cooling fluid flows in a first direction (D1) and in the main portion the cooling fluid flows in a second direction (D2) different from the first direction (D1);wherein the first direction (D1) is from the upstream end towards the downstream end and the second direction (D2) is from the downstream end towards the upstream end ; characterised in that the bent portion (45; 49) is arranged at the downstream end . an outer wall ; an inner wall defining a transition channel ; at least one first convective cooling channel arranged between the inner wall and the outer wall ; the at least one first convective cooling channel having at least one first inlet (46; 50) made in the outer wall between the upstream end and the downstream end in communication with a source of cooling fluid ; the at least one first convective cooling channel comprising at least one inlet portion (43; 48) comprising the at least one first inlet (46; 50), a main portion and at least one bent portion (45; 49) arranged between the at least one inlet portion (43; 48) and the main portion ; wherein in the at least one inlet portion (43; 48) the cooling fluid flows in a first direction (D1) and in the main portion the cooling fluid flows in a second direction (D2) different from the first direction (D1);wherein the first direction (D1) is from the upstream end towards the downstream end and the second direction (D2) is from the downstream end towards the upstream end ; characterised in that the bent portion (45; 49) is arranged at the downstream end .
A fuel composition comprising: a base fuel comprising gasoline or a gasoline-based fuel; a lubricity additive package; and wherein the lubricity additive package comprises Dodecanoic acid and 1-Lauroyl-rac-glycerol, wherein the lubricity additive package is blended with the base fuel at a concentration of 5 ppm to 100 ppm by weight, based on the total weight of the fuel composition, and wherein the treat rate of 1 -lauroyl-rac-glycerol in ppm weight by volume is at least double the treat rate of Dodecanoic acid in ppm weight by volume. a base fuel comprising gasoline or a gasoline-based fuel; a lubricity additive package; and wherein the lubricity additive package comprises Dodecanoic acid and 1-Lauroyl-rac-glycerol, wherein the lubricity additive package is blended with the base fuel at a concentration of 5 ppm to 100 ppm by weight, based on the total weight of the fuel composition, and wherein the treat rate of 1 -lauroyl-rac-glycerol in ppm weight by volume is at least double the treat rate of Dodecanoic acid in ppm weight by volume.