A method comprising the steps of: generating a plurality of sinograms, each being generated by sampling a plurality of transducers acoustically coupled with a surface of a volume for a predetermined period of time after a pulse of light having a predominant wavelength selected from at least two different predominant wavelengths, each transducer being associated with a channel in an optoacoustic imaging system; processing at least two sinograms, each corresponding to a different one of the at least two different predominant wavelengths to create at least two processed sinograms, the processing comprising one or more of: mitigating anomalous channels, mitigating common mode stripes, bandpass filtering, mitigating the system transfer function, normalization of dynamic range, normalization of energy, removal of interframe persistent artifacts; compensating for hardware time gain compensation; performing sub-band acoustic compensation and applying a transform operator; performing image reconstruction based upon the at least two processed sinograms to generate at least two optoacoustic images, the image reconstruction comprising one or more of: extract quadrature, sub-band acoustic compensation, and reconstruction; performing image post processing on the at least two optoacoustic images to generate at least two envelope images, the image post processing comprising one or more of: remove interframe persistent artifact, fluence compensation and complex magnitude; generating an oxygenation map based upon the information contained in the at least two envelope images; generating a hemoglobin map based upon the information contained in each of the at least two envelope images; and generating a transparency masked oxygenation map, the transparency masked oxygenation map reflective of a combination of information in the oxygenation map and the hemoglobin map. generating a plurality of sinograms, each being generated by sampling a plurality of transducers acoustically coupled with a surface of a volume for a predetermined period of time after a pulse of light having a predominant wavelength selected from at least two different predominant wavelengths, each transducer being associated with a channel in an optoacoustic imaging system; processing at least two sinograms, each corresponding to a different one of the at least two different predominant wavelengths to create at least two processed sinograms, the processing comprising one or more of: mitigating anomalous channels, mitigating common mode stripes, bandpass filtering, mitigating the system transfer function, normalization of dynamic range, normalization of energy, removal of interframe persistent artifacts; compensating for hardware time gain compensation; performing sub-band acoustic compensation and applying a transform operator; performing image reconstruction based upon the at least two processed sinograms to generate at least two optoacoustic images, the image reconstruction comprising one or more of: extract quadrature, sub-band acoustic compensation, and reconstruction; performing image post processing on the at least two optoacoustic images to generate at least two envelope images, the image post processing comprising one or more of: remove interframe persistent artifact, fluence compensation and complex magnitude; generating an oxygenation map based upon the information contained in the at least two envelope images; generating a hemoglobin map based upon the information contained in each of the at least two envelope images; and generating a transparency masked oxygenation map, the transparency masked oxygenation map reflective of a combination of information in the oxygenation map and the hemoglobin map. <EOS>