A method for preparing an immobilized enzyme material, the method comprising: providing an aqueous suspension or solution of an enzyme, whereby this has been obtained by producing an enzyme from a first biomass material and preparing an aqueous suspension or solution of said enzyme; and providing a second biomass material and at least two polyfunctional materials, wherein the second biomass material is different from the first biomass material, wherein the second biomass material is selected from the group consisting of bacterial cell material, fungal cell material, cotton, exoskeleton and wool, and wherein said material is not chemically treated or chemically processed, and wherein said material may optionally be mechanically processed; and wherein said at least two polyfunctional materials react with the enzyme to form a crosslinked enzyme; and then adding the second biomass material and one of the two polyfunctional materials to the aqueous suspension or solution of the enzyme to form a reaction mixture; and adding the second polyfunctional material, which is a crosslinking material, to the reaction mixture to form a flocculated immobilized enzyme material that comprises the enzyme in crosslinked form, said crosslinked enzyme being attached to a support matrix that includes the second biomass material and crosslinkers, wherein the method further involves isolating, drying, and formulating the flocculated immobilized enzyme material into particles. providing an aqueous suspension or solution of an enzyme, whereby this has been obtained by producing an enzyme from a first biomass material and preparing an aqueous suspension or solution of said enzyme; and providing a second biomass material and at least two polyfunctional materials, wherein the second biomass material is different from the first biomass material, wherein the second biomass material is selected from the group consisting of bacterial cell material, fungal cell material, cotton, exoskeleton and wool, and wherein said material is not chemically treated or chemically processed, and wherein said material may optionally be mechanically processed; and wherein said at least two polyfunctional materials react with the enzyme to form a crosslinked enzyme; and wherein the second biomass material is different from the first biomass material, wherein the second biomass material is selected from the group consisting of bacterial cell material, fungal cell material, cotton, exoskeleton and wool, and wherein said material is not chemically treated or chemically processed, and wherein said material may optionally be mechanically processed; and wherein said at least two polyfunctional materials react with the enzyme to form a crosslinked enzyme; and then adding the second biomass material and one of the two polyfunctional materials to the aqueous suspension or solution of the enzyme to form a reaction mixture; and adding the second polyfunctional material, which is a crosslinking material, to the reaction mixture to form a flocculated immobilized enzyme material that comprises the enzyme in crosslinked form, said crosslinked enzyme being attached to a support matrix that includes the second biomass material and crosslinkers, wherein the method further involves isolating, drying, and formulating the flocculated immobilized enzyme material into particles. <EOS>