A method for simultaneous determination of microparticle concentration and their morphological and physiological traits in a sample, said method comprising the steps of: - Providing said sample in a sensing chamber with a detection system; - Exposing said sample to predefined excitation wavelengths in said sensing chamber; - Simultaneously detecting emitted signals of said sample in reaction of said exposing step, wherein an area of detection comprises an area of maximum sensitivity of a detector; - Calculating an average signal intensity on the basis of the detected signals, wherein said average signal intensity corresponds to a microparticle concentration; and - Determining the microparticle concentration of said sample and their morphological and physiological traits in a simultaneous manner on the basis of said average signal intensity wherein said determining step comprises determining the microparticle concentration of said sample on the basis of said average signal intensity, wherein the detected signals bear the information about the composition, size, morphological and physiological characteristics of the micro-particles currently located in the area of maximum sensitivity of the detector wherein said providing step comprises obtaining said sample by delivering it in said sensing chamber adapted to create a uniform distribution of particles maintaining them in suspension and forcing them in an unidirectional flow that passes said detection system, said uniform distribution and said unidirectional flow being provided by means of a mixing device of said system that mixes the sample in the sensing chamber thereby providing that the sample passes the area of detection at a constant adjustable speed. - Providing said sample in a sensing chamber with a detection system; - Exposing said sample to predefined excitation wavelengths in said sensing chamber; - Simultaneously detecting emitted signals of said sample in reaction of said exposing step, wherein an area of detection comprises an area of maximum sensitivity of a detector; - Calculating an average signal intensity on the basis of the detected signals, wherein said average signal intensity corresponds to a microparticle concentration; and - Determining the microparticle concentration of said sample and their morphological and physiological traits in a simultaneous manner on the basis of said average signal intensity wherein said determining step comprises determining the microparticle concentration of said sample on the basis of said average signal intensity, wherein the detected signals bear the information about the composition, size, morphological and physiological characteristics of the micro-particles currently located in the area of maximum sensitivity of the detector wherein said providing step comprises obtaining said sample by delivering it in said sensing chamber adapted to create a uniform distribution of particles maintaining them in suspension and forcing them in an unidirectional flow that passes said detection system, said uniform distribution and said unidirectional flow being provided by means of a mixing device of said system that mixes the sample in the sensing chamber thereby providing that the sample passes the area of detection at a constant adjustable speed. <EOS>