An imaging system comprising: a microscope objective ; first and second imaging channels ; a beam splitter and filter assembly that directs different wavelengths of light to the first imaging channel and the second imaging channel ; and a sample positioning X-Y stage , the X-Y stage being independently rotatable in a plane normal to the axis of rotational symmetry and independently translatable in X and Y in the plane normal to the axis; wherein the first and the second imaging channel each contain: a time delay integration camera configured to produce an image from scanning of a sample comprising fluorescently tagged biomolecules along a scanning axis normal to an axis of rotational symmetry of the microscope objective; a lateral offset plate along the axis of rotational symmetry , the lateral offset plate configured to independently shift the position of the image in the time delay integration camera in a plane normal to the scanning axis ; a zoom lens system comprising a tube lens and a helper lens configured to change a scale of the image directed to the time delay integration camera along the axis of rotational symmetry ; and a rotational stage that supports the time delay integration camera , the rotational stage being rotatable around the axis of rotational symmetry . a microscope objective ; first and second imaging channels ; a beam splitter and filter assembly that directs different wavelengths of light to the first imaging channel and the second imaging channel ; and a sample positioning X-Y stage , the X-Y stage being independently rotatable in a plane normal to the axis of rotational symmetry and independently translatable in X and Y in the plane normal to the axis; wherein the first and the second imaging channel each contain: a time delay integration camera configured to produce an image from scanning of a sample comprising fluorescently tagged biomolecules along a scanning axis normal to an axis of rotational symmetry of the microscope objective; a lateral offset plate along the axis of rotational symmetry , the lateral offset plate configured to independently shift the position of the image in the time delay integration camera in a plane normal to the scanning axis ; a zoom lens system comprising a tube lens and a helper lens configured to change a scale of the image directed to the time delay integration camera along the axis of rotational symmetry ; and a rotational stage that supports the time delay integration camera , the rotational stage being rotatable around the axis of rotational symmetry . a time delay integration camera configured to produce an image from scanning of a sample comprising fluorescently tagged biomolecules along a scanning axis normal to an axis of rotational symmetry of the microscope objective; a lateral offset plate along the axis of rotational symmetry , the lateral offset plate configured to independently shift the position of the image in the time delay integration camera in a plane normal to the scanning axis ; a zoom lens system comprising a tube lens and a helper lens configured to change a scale of the image directed to the time delay integration camera along the axis of rotational symmetry ; and a rotational stage that supports the time delay integration camera , the rotational stage being rotatable around the axis of rotational symmetry . <EOS>