mhurhangee/patent-ind-claim-en huggingface dataset:
A method for inserting at least one polynucleotide of interest into the genome of a host cell, the method comprising the steps of: a) providing a host cell comprising in its genome: i. a polynucleotide encoding a selectable marker comprising a target sequence flanked by a functional PAM sequence for a Class-II Cas9 protein; ii. at least one polynucleotide encoding a gRNA that is at least 80% complementary to and capable of hybridizing to the target sequence; and iii. a polynucleotide encoding a nuclease-null variant of a Class-II Cas9 protein capable of interaction with the gRNA and binding to the target sequence, whereby expression of the selectable marker is repressed; b) transforming said host cell with at least one polynucleotide of interest and capable of inactivating the at least one polynucleotide encoding the gRNA; c) selecting for the trait conferred by the selectable marker; and d) identifying a transformed host cell, wherein the at least one polynucleotide encoding the gRNA has been inactivated by the at least one polynucleotide of interest. a) providing a host cell comprising in its genome: i. a polynucleotide encoding a selectable marker comprising a target sequence flanked by a functional PAM sequence for a Class-II Cas9 protein; ii. at least one polynucleotide encoding a gRNA that is at least 80% complementary to and capable of hybridizing to the target sequence; and iii. a polynucleotide encoding a nuclease-null variant of a Class-II Cas9 protein capable of interaction with the gRNA and binding to the target sequence, whereby expression of the selectable marker is repressed; i. a polynucleotide encoding a selectable marker comprising a target sequence flanked by a functional PAM sequence for a Class-II Cas9 protein; ii. at least one polynucleotide encoding a gRNA that is at least 80% complementary to and capable of hybridizing to the target sequence; and iii. a polynucleotide encoding a nuclease-null variant of a Class-II Cas9 protein capable of interaction with the gRNA and binding to the target sequence, whereby expression of the selectable marker is repressed; b) transforming said host cell with at least one polynucleotide of interest and capable of inactivating the at least one polynucleotide encoding the gRNA; c) selecting for the trait conferred by the selectable marker; and d) identifying a transformed host cell, wherein the at least one polynucleotide encoding the gRNA has been inactivated by the at least one polynucleotide of interest.
Method for displaying information on a display device , comprising the steps of: - determining an item of information to be displayed, - determining that a user is looking at the display device , and, in response thereto, - displaying a message , representing the information, on the display device , characterized in that the method further comprises - determining that the user is looking at the display device again, and, in response thereto, - displaying the message again. - determining an item of information to be displayed, - determining that a user is looking at the display device , and, in response thereto, - displaying a message , representing the information, on the display device , characterized in that the method further comprises - determining that the user is looking at the display device again, and, in response thereto, - displaying the message again.
For a host computer in a plurality of host computers executing a plurality of virtual machines , the plurality of host computers implementing a plurality of logical networks on a shared network infrastructure of a datacenter, a method of encrypting data messages on a host computer of the plurality of host computers, the method comprising: at the host computer: receiving data messages for the plurality of logical networks, wherein each logical network comprises a logical forwarding element that spans a plurality of host computers to connect a set of machines in the logical network, the logical forwarding element implemented by a plurality of physical forwarding elements executing on the plurality of host computers , each logical forwarding element identified by a logical-forwarding-element identifier; for each particular logical network, using the logical-forwarding-element identifier for the logical forwarding element to identify an encryption parameter for an encryption process; and for each particular logical network, using the identified encryption parameters to encrypt the data messages, wherein the data messages for at least two different logical networks are encrypted differently. receiving data messages for the plurality of logical networks, wherein each logical network comprises a logical forwarding element that spans a plurality of host computers to connect a set of machines in the logical network, the logical forwarding element implemented by a plurality of physical forwarding elements executing on the plurality of host computers , each logical forwarding element identified by a logical-forwarding-element identifier; for each particular logical network, using the logical-forwarding-element identifier for the logical forwarding element to identify an encryption parameter for an encryption process; and for each particular logical network, using the identified encryption parameters to encrypt the data messages, wherein the data messages for at least two different logical networks are encrypted differently.
A medical image processing apparatus comprising: a first image acquirer that acquires a plurality of first images of an object to be treated, each of the plurality of first images being captured by a radiographic imaging apparatus at a respective time of a plurality of times; a second image acquirer that acquires a second image of the object to be treated ,the second image being captured by a radiographic imaging apparatus at a time different from the plurality of times when the plurality of first images are captured; a characteristic processor that determines a plurality of positions of a first characteristic of the object to be treated from the plurality of first images, the characteristic processor that determines the position of a second characteristic corresponding to the first characteristic in the object to be treated from the second image; a calculator that establishes a first closed space including the plurality of positions of the first characteristic; and a determiner that determines whether or not the position of the second characteristic is within the first closed space, and outputs a determination signal, based on a result of the determination. a first image acquirer that acquires a plurality of first images of an object to be treated, each of the plurality of first images being captured by a radiographic imaging apparatus at a respective time of a plurality of times; a second image acquirer that acquires a second image of the object to be treated ,the second image being captured by a radiographic imaging apparatus at a time different from the plurality of times when the plurality of first images are captured; a characteristic processor that determines a plurality of positions of a first characteristic of the object to be treated from the plurality of first images, the characteristic processor that determines the position of a second characteristic corresponding to the first characteristic in the object to be treated from the second image; a calculator that establishes a first closed space including the plurality of positions of the first characteristic; and a determiner that determines whether or not the position of the second characteristic is within the first closed space, and outputs a determination signal, based on a result of the determination.
A communication circuit of an electronic device comprising: a plurality of duplexers including a first duplexer and a second duplexer , wherein each of the plurality of duplexers includes a transmission, 'Tx', port for inputting a Tx signal of a first communication standard and for outputting a reception, Rx, signal of a second communication standard, and a reception, 'Rx' port for outputting a Rx signal of a first communication standard; a first switch configured to connect any one of the plurality of duplexers to an antenna ; and a second switch configured to connect a first port , to which the Tx signal of the first communication standard or a Tx signal of the second communication standard is input, to any one of Tx ports of the first and second duplexers, and to connect a second port , from which the Rx signal of the second communication standard is output, to a Tx port of a duplexer of the first and second duplexers, wherein the first communication standard supports frequency division multiplexing, the second communication standard supports time division multiplexing, wherein a frequency band used by the second communication standard corresponds to a transmission frequency band of the first communication standard. a plurality of duplexers including a first duplexer and a second duplexer , wherein each of the plurality of duplexers includes a transmission, 'Tx', port for inputting a Tx signal of a first communication standard and for outputting a reception, Rx, signal of a second communication standard, and a reception, 'Rx' port for outputting a Rx signal of a first communication standard; a first switch configured to connect any one of the plurality of duplexers to an antenna ; and a second switch configured to connect a first port , to which the Tx signal of the first communication standard or a Tx signal of the second communication standard is input, to any one of Tx ports of the first and second duplexers, and to connect a second port , from which the Rx signal of the second communication standard is output, to a Tx port of a duplexer of the first and second duplexers, wherein the first communication standard supports frequency division multiplexing, the second communication standard supports time division multiplexing, wherein a frequency band used by the second communication standard corresponds to a transmission frequency band of the first communication standard.
A solenoid fluid control valve comprising: a transceiver configured to receive a signal via a controller area network (CAN) bus ; the transceiver further configured to forward the signal received via the CAN bus to a micro controller unit ; the micro controller unit configured to decode the signal received from the transceiver to determine a current temperature, to compare the current temperature to a pre-determined threshold temperature, and to encode and send a signal to the transceiver ; the transceiver configured to broadcast the signal received from the micro controller unit over the CAN bus ; the micro controller unit further configured to send a signal to a driver circuit based on the temperature; the driver circuit configured to send a current through a coil in response to the signal received from the micro controller unit ; and an armature configured to move in response to the current through the coil , wherein the movement of the armature directs a cooling fluid flow . a transceiver configured to receive a signal via a controller area network (CAN) bus ; the transceiver further configured to forward the signal received via the CAN bus to a micro controller unit ; the micro controller unit configured to decode the signal received from the transceiver to determine a current temperature, to compare the current temperature to a pre-determined threshold temperature, and to encode and send a signal to the transceiver ; the transceiver configured to broadcast the signal received from the micro controller unit over the CAN bus ; the micro controller unit further configured to send a signal to a driver circuit based on the temperature; the driver circuit configured to send a current through a coil in response to the signal received from the micro controller unit ; and an armature configured to move in response to the current through the coil , wherein the movement of the armature directs a cooling fluid flow .
A non-aqueous inkjet composition comprising: a pigment containing a diketopyrrolopyrrole red pigment; a vinyl chloride resin; and an organic solvent containing a glycol diether represented by Formula and a glycol monoether represented by Formula : R 1 O-(R 3 O) m -R 2 where, R 1 and R 2 each independently represent an alkyl group having 1 to 7 carbon atoms, R 3 represents an alkylene group having 1 to 3 carbon atoms, and m represents an integer of 1 to 7, OH-(R 5 O) n -R 4 where, R 4 represents an alkyl group having 1 to 7 carbon atoms, R 5 represents an alkylene group having 1 to 3 carbon atoms, and n represents an integer of 1 to 7. a pigment containing a diketopyrrolopyrrole red pigment; a vinyl chloride resin; and an organic solvent containing a glycol diether represented by Formula and a glycol monoether represented by Formula : R 1 O-(R 3 O) m -R 2 where, R 1 and R 2 each independently represent an alkyl group having 1 to 7 carbon atoms, R 3 represents an alkylene group having 1 to 3 carbon atoms, and m represents an integer of 1 to 7, OH-(R 5 O) n -R 4 where, R 4 represents an alkyl group having 1 to 7 carbon atoms, R 5 represents an alkylene group having 1 to 3 carbon atoms, and n represents an integer of 1 to 7.
Method for producing an adhesive bond between at least one first and second, at least partially transparent, plate-like component (9; 12), in which the first component is placed onto a supporting plate , in which adhesive is applied to the first component at specified locations on the side of the first component which faces away from the supporting plate , in which the second component is placed onto the first component and is held parallel to the first component at a predetermined distance (D), namely in such a manner that the second component comes into contact with the adhesive located on the first component , wherein to cure the adhesive , this adhesive is exposed to light from the side of the supporting plate facing away from the components (9; 12) through the at least partially transparent supporting plate and through the first component , characterized in that the first component is placed onto raised structures of the supporting plate .
A device for knotting a thread end such that a loop is formed, in particular a thread end of a retrieval thread for tampons, comprising: a. a knotter assembly for knotting two thread sections of the thread end; b. a driver for feeding the thread sections to the knotter assembly ; c. an orbital path along which the driver is guided around the knotter assembly , and characterized in that the orbital path has a knotting region (V) in which the driver is movable at a first speed , and an envelope region (U) in which the driver is movable at a second speed , and further comprising a holding device , designed as a latch, which is designed to exert a restoring force on a loop end of the thread end to be knotted. a. a knotter assembly for knotting two thread sections of the thread end; b. a driver for feeding the thread sections to the knotter assembly ; c. an orbital path along which the driver is guided around the knotter assembly , and the orbital path has a knotting region (V) in which the driver is movable at a first speed , and an envelope region (U) in which the driver is movable at a second speed , and further comprising a holding device , designed as a latch, which is designed to exert a restoring force on a loop end of the thread end to be knotted.
A method of fabricating a preceramic polymer for making a ceramic material including a metal boride, the method comprising: providing a starting preceramic polymer that includes a silicon-containing backbone chain and first and second reactive side groups extending off of the silicon-containing backbone chain, wherein the first reactive side group is a N-H moiety and the second reactive side group is a Si-H moiety; reacting a boron-containing material with the N-H moiety to bond a boron moiety to the silicon-containing backbone chain; and reacting a metal-containing material with the Si-H moiety to bond a metal moiety to the silicon-containing backbone chain such that the preceramic polymer includes the boron moiety and the metal moiety extending as side groups off of silicon atoms in the silicon-containing backbone chain. providing a starting preceramic polymer that includes a silicon-containing backbone chain and first and second reactive side groups extending off of the silicon-containing backbone chain, wherein the first reactive side group is a N-H moiety and the second reactive side group is a Si-H moiety; reacting a boron-containing material with the N-H moiety to bond a boron moiety to the silicon-containing backbone chain; and reacting a metal-containing material with the Si-H moiety to bond a metal moiety to the silicon-containing backbone chain such that the preceramic polymer includes the boron moiety and the metal moiety extending as side groups off of silicon atoms in the silicon-containing backbone chain.
A method comprising: obtaining, at a controller (102-1) of a software defined network , SDN, at least one security policy from a policy creator; and implementing, via the controller, the security policy in the software defined network based on one or more attributes specifying a characteristic of the security policy, a role of the creator of the security policy, and a security privilege level of the role of the creator of the security policy; wherein the characteristic of the security policy attribute describes whether the security policy is a real-time policy or can be put into operation with some delay; translating the obtained at least one security policy into flow rules reflecting at least the one or more attributes; updating the one or more flow rules in one or more flow tables of the controller; synchronizing the one or more flow rules with related SDN switches in the software defined network depending on the security policy attribute, wherein the synchronization of the one or more flow rules is realized in real-time for the real-time policy and the synchronization of the one or more flow rules is realized periodically for the security policy that can be put into operation with some delay.
A sample extraction system capable of providing sustained delivery of feed solution to a system, the sample extraction system comprising: (a) an extraction solvent source , wherein the extraction solvent comprises a fluid selected from a group consisting of (i) an organic solvent maintained at or above 86.2 bar (1250 psi) and (ii) CO2; (b) a splitter in fluid communication with the extraction solvent source, the splitter for providing two or more fluid paths downstream of the splitter; (c) a first extraction vessel assembly and a second extraction vessel assembly , each extraction vessel assembly comprising a first valve , an inlet port , an outlet port , and a second valve , the first valve being connected to the splitter, the inlet port being connected to the first valve and to an inlet of an extraction vessel, and the outlet port being connected to an outlet of the extraction vessel and being connected to the second valve; (d) a coupler , in fluid communication with the second valve of each of the first extraction vessel assembly and the second valve of the second extraction assembly, the coupler for receiving fluid flow from the two or more fluid paths upstream and delivering combined flow to a single stream downstream of the coupler; and (e) a feed injector in fluid communication with the coupler, the feed injector capable of providing a feed of extraction solvent supplied with sample for use in the system. (a) an extraction solvent source , wherein the extraction solvent comprises a fluid selected from a group consisting of (i) an organic solvent maintained at or above 86.2 bar (1250 psi) and (ii) CO2; (b) a splitter in fluid communication with the extraction solvent source, the splitter for providing two or more fluid paths downstream of the splitter; (c) a first extraction vessel assembly and a second extraction vessel assembly , each extraction vessel assembly comprising a first valve , an inlet port , an outlet port , and a second valve , the first valve being connected to the splitter, the inlet port being connected to the first valve and to an inlet of an extraction vessel, and the outlet port being connected to an outlet of the extraction vessel and being connected to the second valve; (d) a coupler , in fluid communication with the second valve of each of the first extraction vessel assembly and the second valve of the second extraction assembly, the coupler for receiving fluid flow from the two or more fluid paths upstream and delivering combined flow to a single stream downstream of the coupler; and (e) a feed injector in fluid communication with the coupler, the feed injector capable of providing a feed of extraction solvent supplied with sample for use in the system.
A compound, or a pharmaceutically acceptable salt thereof, having a structure of: wherein Y 2 , Y 3 , Y 4 and Y 5 are each independently H, optionally-substituted alkyl, amino, hydroxyl, optionally-substituted alkoxy, optionally-substituted thiol, acyl, or halogen, wherein the substituent if present in the optionally-substituted alkyl, optionally-substituted alkoxy, or optionally-substituted thiol is selected from C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, C 3-8 cycloalkyl, hydroxyl, oxo, C 1-6 alkoxy, aryloxy, C 1-6 alkoxyaryl, halo, C 1-6 alkylhalo, C 1-6 alkoxyhalo, carboxyl, esters, cyano, nitro, amino, substituted amino, disubstituted amino, acyl, ketone, amide, aminoacyl, substituted amide, disubstituted amide, thiol, alkylthio, thioxo, sulfate, sulfonate, sulfinyl, substituted sulfinyl, sulfonyl, substituted sulfonyl, sulfonylamide, substituted sulfonamide, disubstituted sulfonamide, aryl, arC 1-6 alkyl, heterocyclyl or heteroaryl; and wherein -S-R 5 is a derivative of N-acetylcysteine, 2-mercaptoethane sulfonate, or glutathione. wherein Y 2 , Y 3 , Y 4 and Y 5 are each independently H, optionally-substituted alkyl, amino, hydroxyl, optionally-substituted alkoxy, optionally-substituted thiol, acyl, or halogen, wherein the substituent if present in the optionally-substituted alkyl, optionally-substituted alkoxy, or optionally-substituted thiol is selected from C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, C 3-8 cycloalkyl, hydroxyl, oxo, C 1-6 alkoxy, aryloxy, C 1-6 alkoxyaryl, halo, C 1-6 alkylhalo, C 1-6 alkoxyhalo, carboxyl, esters, cyano, nitro, amino, substituted amino, disubstituted amino, acyl, ketone, amide, aminoacyl, substituted amide, disubstituted amide, thiol, alkylthio, thioxo, sulfate, sulfonate, sulfinyl, substituted sulfinyl, sulfonyl, substituted sulfonyl, sulfonylamide, substituted sulfonamide, disubstituted sulfonamide, aryl, arC 1-6 alkyl, heterocyclyl or heteroaryl; and wherein -S-R 5 is a derivative of N-acetylcysteine, 2-mercaptoethane sulfonate, or glutathione.