A method of manufacturing an electrophoretic display module with double-layer microstructure, comprising the following steps: step 1: preparing a plasma barrier array on a pixel electrode in advance; step 2: embedding the pixel electrode in a TFT glass substrate , and placing the TFT glass substrate on a dispensing platform; step 3: applying a sealing adhesive on the pixel electrode to form a liner frame which surrounds the pixel electrode ; step 4: silk screening a display plasma comprising electrophoretic particles and spraying spacer particles within the space surrounded by the liner frame , wherein the relatively light spacer particles float on the display plasma ; step 5: coating a conductive silver paste in the liner frame , wherein the conductive silver paste is electrically connected to the pixel electrode ; step 6: firstly, laminating a display area protective layer on the whole liner frame , secondly, laminating a transparent electrode on the liner frame and the display plasma , wherein, in the process, most of the spacer particles are squeezed, transferred, and adsorbed on the plasma barrier frame of the plasma barrier array to form the spacer particle layer , and then solidifying; step 7: cutting off edges of the transparent electrode and the display area protective layer to expose a position predetermined to bind an IC integrated circuit module and a flexible circuit board on the pixel electrode step 8: adhering the IC integrated circuit module and the flexible circuit board to an edge of the pixel electrode through a conductive adhesive tape; and step 9: firmly sealing peripheries of the IC integrated circuit module , the flexible circuit board and the conductive adhesive tape on the pixel electrode by a blue adhesive , thus completing manufacturing of the electronic ink display screen. step 1: preparing a plasma barrier array on a pixel electrode in advance; step 2: embedding the pixel electrode in a TFT glass substrate , and placing the TFT glass substrate on a dispensing platform; step 3: applying a sealing adhesive on the pixel electrode to form a liner frame which surrounds the pixel electrode ; step 4: silk screening a display plasma comprising electrophoretic particles and spraying spacer particles within the space surrounded by the liner frame , wherein the relatively light spacer particles float on the display plasma ; step 5: coating a conductive silver paste in the liner frame , wherein the conductive silver paste is electrically connected to the pixel electrode ; step 6: firstly, laminating a display area protective layer on the whole liner frame , secondly, laminating a transparent electrode on the liner frame and the display plasma , wherein, in the process, most of the spacer particles are squeezed, transferred, and adsorbed on the plasma barrier frame of the plasma barrier array to form the spacer particle layer , and then solidifying; step 7: cutting off edges of the transparent electrode and the display area protective layer to expose a position predetermined to bind an IC integrated circuit module and a flexible circuit board on the pixel electrode step 8: adhering the IC integrated circuit module and the flexible circuit board to an edge of the pixel electrode through a conductive adhesive tape; and step 9: firmly sealing peripheries of the IC integrated circuit module , the flexible circuit board and the conductive adhesive tape on the pixel electrode by a blue adhesive , thus completing manufacturing of the electronic ink display screen. <EOS>