mhurhangee/patent-ind-claim-en huggingface dataset:
A method for separating and culturing umbilical cord mesenchymal stem cells, the method comprising: cutting Wharton's jelly tissue separated from umbilical cord into tissue blocks, adding red blood cell lysis buffer with a volume of 1-3 times the volume of the tissue blocks, and treating the tissue blocks with the buffer at room temperature for 2-5 minutes; and culturing the obtained tissue blocks with serum-free medium for mesenchymal stem cells to obtain primary mesenchymal stem cells; and wherein the red blood cell lysis buffer is an aqueous solution consisting of 1-20 g/L NH 4 Cl and 0.05-0.2 mM Na 2 -EDTA, and the serum-free medium for mesenchymal stem cells comprises 0.05-0.2 parts by volume of β-mercapto ethanol, 0.5-2 parts by volume of an aqueous solution of non-essential amino acids, 8-12 parts by volume of serum substitute, 85-95 parts by volume of a-MEM/DMEM-F12 and recombinant human basic fibroblast growth factor at a final concentration of 5-15 ng/ml, wherein the aqueous solution of non-essential amino acids comprises glycine, alanine, L-asparagine, L-aspartic acid, glutamic acid, proline and serine each at a concentration of 8-12 mM. cutting Wharton's jelly tissue separated from umbilical cord into tissue blocks, adding red blood cell lysis buffer with a volume of 1-3 times the volume of the tissue blocks, and treating the tissue blocks with the buffer at room temperature for 2-5 minutes; and culturing the obtained tissue blocks with serum-free medium for mesenchymal stem cells to obtain primary mesenchymal stem cells; and wherein the red blood cell lysis buffer is an aqueous solution consisting of 1-20 g/L NH 4 Cl and 0.05-0.2 mM Na 2 -EDTA, and the serum-free medium for mesenchymal stem cells comprises 0.05-0.2 parts by volume of β-mercapto ethanol, 0.5-2 parts by volume of an aqueous solution of non-essential amino acids, 8-12 parts by volume of serum substitute, 85-95 parts by volume of a-MEM/DMEM-F12 and recombinant human basic fibroblast growth factor at a final concentration of 5-15 ng/ml, wherein the aqueous solution of non-essential amino acids comprises glycine, alanine, L-asparagine, L-aspartic acid, glutamic acid, proline and serine each at a concentration of 8-12 mM.
A device for closing and opening doors and/or hatches and/or similar components, in particular of electrical household appliances such as washing machines, dish washers, and the like, said device comprising a main unit or body, which houses a closing and opening element adapted to be engaged and disengaged alternatively by a latch or catch adapted to be rigidly fixed to said door, wherein said closing and opening element can be switched by rotation between a first, opening, position and a second, closing, position, wherein the mutual engagement by thrust of said catch against said closing and opening element results first in said closing and opening element being switched from said first, opening, position to said second, closing, position, said device comprising elastic means arranged between said closing and opening element and said main unit or body, wherein said closing and opening element can be switched by rotation between said first, opening, position and said second, closing, position about a rotation pin of said closing and opening element , which is housed at least partially engaging in a housing and engagement seat of said main unit or body, wherein said housing and engagement seat is shaped so as to enable not only the rotation of said rotation pin but also the translation of said rotation pin , wherein said housing and engagement seat is delimited by an inner surface substantially parallel to the direction of longitudinal extension of said rotation pin , characterized in that said housing and engagement seat has an oblong conformation and defines two end portions , and in that, with said closing and opening element in said first opening position, said rotation pin engages a first end portion of said housing and engagement seat , whereas, with said closing and opening element in said second closing position, said rotation pin engages the second end portion of said housing and engagement seat opposite to said first end portion , in that at said first end portion the intersections of the generatrices of said inner surface with a plane perpendicular thereto lie on a first arc of circle with substantially constant radius of curvature, in that in correspondence of said second end portion the intersections of the generatrices of said inner surface with a plane perpendicular thereto lie on a second arc of circle with substantially constant radius of curvature smaller than that of said first arc of circle, in that said rotation pin is delimited by an outer surface substantially parallel to the direction of longitudinal extension of said rotation pin , which comprises a first portion and a second portion connected together, and in that the intersections of the generatrices of said first portion with a plane perpendicular thereto lie on a third arc of circle while the intersections of the generatrices of said second portion with a plane perpendicular thereto lie on a fourth half-line.
A computer-implemented method comprising: receiving a request at a data center of a Program Execution Service, PES, from a computing device to access a virtual desktop instance, the request identifying a user of the computing device; identifying a first virtual desktop instance corresponding to the request; causing a desktop store to attach to the first virtual desktop instance, the desktop store including a desktop profile for the user, wherein a first operating system running within the first virtual desktop instance is personalized according to the desktop profile for the user and one or more operating system folders are mapped to storage in the desktop store for the user; providing, to the computing device, access to the first virtual desktop instance; determining an event related to the first virtual desktop instance, wherein the event related to the first virtual desktop instance prevents the computing device from accessing the first virtual desktop instance; identifying a second virtual desktop instance corresponding to the request; and causing the desktop store to detach from the first virtual desktop instance and to attach to the second virtual desktop instance, wherein a second operating system running within the second virtual desktop instance is personalized according to the desktop profile for the user and to one or more operating system folders mapped to storage in the desktop store for the user; and providing, to the computing device, access to a second virtual desktop instance. receiving a request at a data center of a Program Execution Service, PES, from a computing device to access a virtual desktop instance, the request identifying a user of the computing device; identifying a first virtual desktop instance corresponding to the request; causing a desktop store to attach to the first virtual desktop instance, the desktop store including a desktop profile for the user, wherein a first operating system running within the first virtual desktop instance is personalized according to the desktop profile for the user and one or more operating system folders are mapped to storage in the desktop store for the user; providing, to the computing device, access to the first virtual desktop instance; determining an event related to the first virtual desktop instance, wherein the event related to the first virtual desktop instance prevents the computing device from accessing the first virtual desktop instance; identifying a second virtual desktop instance corresponding to the request; and causing the desktop store to detach from the first virtual desktop instance and to attach to the second virtual desktop instance, wherein a second operating system running within the second virtual desktop instance is personalized according to the desktop profile for the user and to one or more operating system folders mapped to storage in the desktop store for the user; and providing, to the computing device, access to a second virtual desktop instance.
A method for measuring substance quantities ( m 1 , m 2 ) in a substance mixture with two substances( M 1 , M 2 ): o determining a first moisture value ( φ 1 ) for a first of the two substances ( M 1 ), and determining a second moisture value ( φ 2 ) for the second of the two substances ( M 2 ) using a microwave resonator, o measuring two measured values (A, B) for the substance mixture using a microwave resonator, wherein one of the two measured values (A) represents a resonance frequency shift, and another measured value (B) represents a resonance curve width change, o calculating two scalar factors ( θ 1 , θ 2 ) for two moisture vectors ( e 1 , e 2 ) from the two measured values (A, B) for the substance mixture, wherein the moisture vectors ( e 1 , e 2 ) each indicate the direction of a straight line of equal moisture for the first and second substance ( M 1 , M 2 ) in a plane of the measured values (A, B) using the first and second moisture value ( φ 1 , φ 2 ), o determining the substance quantities ( m 1 , m 2 ) in the substance mixture from the scalar factors ( θ 1 , θ 2 ). o determining a first moisture value ( φ 1 ) for a first of the two substances ( M 1 ), and determining a second moisture value ( φ 2 ) for the second of the two substances ( M 2 ) using a microwave resonator, o measuring two measured values (A, B) for the substance mixture using a microwave resonator, wherein one of the two measured values (A) represents a resonance frequency shift, and another measured value (B) represents a resonance curve width change, o calculating two scalar factors ( θ 1 , θ 2 ) for two moisture vectors ( e 1 , e 2 ) from the two measured values (A, B) for the substance mixture, wherein the moisture vectors ( e 1 , e 2 ) each indicate the direction of a straight line of equal moisture for the first and second substance ( M 1 , M 2 ) in a plane of the measured values (A, B) using the first and second moisture value ( φ 1 , φ 2 ), o determining the substance quantities ( m 1 , m 2 ) in the substance mixture from the scalar factors ( θ 1 , θ 2 ).
Fire insulation for use on a ship, comprising • a metal wall which is a deck, a bulkhead or a part of the outer shell of a ship, • insulation pins consisting of metal, which are fastened on the metal wall and are substantially aligned vertically to the metal wall, • plate-shaped insulation material which is placed on the insulation pins so that the insulation material is held on the metal wall, characterized in that • the insulation pins have a diameter of less than or equal to 3 mm, • the insulation pins have reinforcement in the region of the fastening to the metal wall , • wherein the reinforcement is a bushing compressed with the insulation pin , which is arranged at the lower end of the insulation pin . • a metal wall which is a deck, a bulkhead or a part of the outer shell of a ship, • insulation pins consisting of metal, which are fastened on the metal wall and are substantially aligned vertically to the metal wall, • plate-shaped insulation material which is placed on the insulation pins so that the insulation material is held on the metal wall, characterized in that • the insulation pins have a diameter of less than or equal to 3 mm, • the insulation pins have reinforcement in the region of the fastening to the metal wall , • wherein the reinforcement is a bushing compressed with the insulation pin , which is arranged at the lower end of the insulation pin .
A clamping assembly comprising: a longitudinal member having a first end and a second end , the second end defining a first clamp ; a second clamp configured to attach to the first end of the longitudinal member and connect to a rod assembly ; a clamping member including a first end , a second end defining a spherical portion, and a third clamp , wherein the first clamp of the longitudinal member is configured to receive the second end of the clamping member, and characterized in that the spherical portion is configured to expand. a longitudinal member having a first end and a second end , the second end defining a first clamp ; a second clamp configured to attach to the first end of the longitudinal member and connect to a rod assembly ; a clamping member including a first end , a second end defining a spherical portion, and a third clamp , wherein the first clamp of the longitudinal member is configured to receive the second end of the clamping member, and characterized in that the spherical portion is configured to expand.
A rinse-off conditioner composition comprising: - a core-shell microcapsules slurry comprising microcapsules having an oil-based core and a polymeric shell coated with at least one cationic polymer; - up to 4wt% by weight of at least one quaternary ammonium salt; - from 0.25 to 15wt% by weight of at least one non quaternized conditioning ingredient comprising an oil or a wax or a mixture thereof; - less than 2% by weight of at least one water soluble cationic conditioning polymer; based on the total weight of the composition, characterized in that the composition is free of anionic, amphoteric or zwitterionic surfactants. - a core-shell microcapsules slurry comprising microcapsules having an oil-based core and a polymeric shell coated with at least one cationic polymer; - up to 4wt% by weight of at least one quaternary ammonium salt; - from 0.25 to 15wt% by weight of at least one non quaternized conditioning ingredient comprising an oil or a wax or a mixture thereof; - less than 2% by weight of at least one water soluble cationic conditioning polymer; based on the total weight of the composition,
A mycothione reductase variant consisting of an amino acid sequence represented by SEQ ID NO: 1, wherein proline, which is an amino acid corresponding to position 358 of SEQ ID NO: 3, is substituted with serine.
A composite substrate comprising: a supporting substrate comprising quartz; a piezoelectric material substrate comprising a material selected from the group consisting of lithium niobate, lithium tantalate and lithium niobate-lithium tantalate; and an interface layer along a bonding interface between said supporting substrate and said piezoelectric material substrate , said interface layer having amorphous structure and comprising constituent components including silicon, oxygen and at least one of tantalum and niobium, characterised in that said interface layer has a concentration of hydrogen atoms of 1×10 18 atoms/cm 3 or higher and 5×10 21 atoms/cm 3 or lower, a concentration of nitrogen atoms of 1×10 18 atoms/cm 3 or higher and 5×10 21 atoms/cm 3 or lower, and a concentration of fluorine atoms of 1×10 18 atoms/cm 3 or higher and 5×10 21 atoms/cm 3 or lower. a supporting substrate comprising quartz; a piezoelectric material substrate comprising a material selected from the group consisting of lithium niobate, lithium tantalate and lithium niobate-lithium tantalate; and an interface layer along a bonding interface between said supporting substrate and said piezoelectric material substrate , said interface layer having amorphous structure and comprising constituent components including silicon, oxygen and at least one of tantalum and niobium, characterised in that said interface layer has a concentration of hydrogen atoms of 1×10 18 atoms/cm 3 or higher and 5×10 21 atoms/cm 3 or lower, a concentration of nitrogen atoms of 1×10 18 atoms/cm 3 or higher and 5×10 21 atoms/cm 3 or lower, and a concentration of fluorine atoms of 1×10 18 atoms/cm 3 or higher and 5×10 21 atoms/cm 3 or lower.
A method for shaping a timepiece mainspring formed from a monolithic ribbon made of a metallic glass, characterized in that : - a theoretical free shape to be given to this monolithic ribbon made of a metallic glass, so that each segment, once the mainspring is fully wound in the barrel, i.e. subjected to the maximum bending moment, is calculated; - this ribbon is shaped, giving it curvatures characteristic of this theoretical free shape, in order to take account of a reduction in the curvatures once the ribbon is freed; - the ribbon is subjected to relaxation in order to fix its shape, by heating it about the glass transition temperature or in a range between Tx+50K and Tg-50K, with Tg being the glass transition temperature and Tx being the crystallization temperature, - this ribbon is cooled. - a theoretical free shape to be given to this monolithic ribbon made of a metallic glass, so that each segment, once the mainspring is fully wound in the barrel, i.e. subjected to the maximum bending moment, is calculated; - this ribbon is shaped, giving it curvatures characteristic of this theoretical free shape, in order to take account of a reduction in the curvatures once the ribbon is freed; - the ribbon is subjected to relaxation in order to fix its shape, by heating it about the glass transition temperature or in a range between Tx+50K and Tg-50K, with Tg being the glass transition temperature and Tx being the crystallization temperature, - this ribbon is cooled.
A transmission method characterised by the steps of: extracting, by a sequence extractor , a first sequence for a non-coherent receiver and a second sequence for a coherent receiver from a ternary preamble sequence including elements -1, 0, and 1, wherein the first sequence includes elements 0 and 1, and the second sequence includes elements -1 and 1, and wherein the step of extracting comprises, extracting the first sequence by converting the elements of the ternary preamble sequence into absolute values based on a period of the first sequence, and extracting the second sequence by converting the element 0 of the ternary preamble sequence into the element 1 based on a period of the second sequence; and generating, by a sequence generator , a third sequence supported by both the non-coherent receiver and the coherent receiver by mapping the first sequence and the second sequence to bits of a preamble for transmitting the third sequence to at least one among the non-coherent receiver and the coherent receiver , wherein the step of generating comprises generating a base preamble sequence corresponding to the bits of the preamble, and generating the third sequence by repeating the base preamble sequence a certain number of times, and wherein the step of generating comprises generating the base preamble sequence by repetitively expanding the first sequence based on a ratio between a period of the first sequence and a period of the second sequence and multiplying the expanded first sequence by the second sequence. extracting, by a sequence extractor , a first sequence for a non-coherent receiver and a second sequence for a coherent receiver from a ternary preamble sequence including elements -1, 0, and 1, wherein the first sequence includes elements 0 and 1, and the second sequence includes elements -1 and 1, and wherein the step of extracting comprises, extracting the first sequence by converting the elements of the ternary preamble sequence into absolute values based on a period of the first sequence, and extracting the second sequence by converting the element 0 of the ternary preamble sequence into the element 1 based on a period of the second sequence; and generating, by a sequence generator , a third sequence supported by both the non-coherent receiver and the coherent receiver by mapping the first sequence and the second sequence to bits of a preamble for transmitting the third sequence to at least one among the non-coherent receiver and the coherent receiver , wherein the step of generating comprises generating a base preamble sequence corresponding to the bits of the preamble, and generating the third sequence by repeating the base preamble sequence a certain number of times, and wherein the step of generating comprises generating the base preamble sequence by repetitively expanding the first sequence based on a ratio between a period of the first sequence and a period of the second sequence and multiplying the expanded first sequence by the second sequence.
A method of producing a thermoformed article, comprising: (a) positioning a detached releasable separator film comprising an average thickness of from about 37 to 75 microns over a male or female dental mold or model; (b) heating a thermoformable sheet material to a temperature greater than the melting point of the detached releasable separator film; (c) thermoforming the heated sheet material over the detached releasable separator film and mold or model in a thermoforming device; (d) separating the thermoformed article from the mold or model; and (e) removing the detached releasable separator film from the thermoformed article and the mold or model, producing a thermoformed article. (a) positioning a detached releasable separator film comprising an average thickness of from about 37 to 75 microns over a male or female dental mold or model; (b) heating a thermoformable sheet material to a temperature greater than the melting point of the detached releasable separator film; (c) thermoforming the heated sheet material over the detached releasable separator film and mold or model in a thermoforming device; (d) separating the thermoformed article from the mold or model; and (e) removing the detached releasable separator film from the thermoformed article and the mold or model, producing a thermoformed article.
A foldable chair for leisure comprises: coupling holes formed at a set angle on both sides of a support tube such that insert tubes of front support tubes are penetrated and coupled through the coupling holes so as to be exposed, backrest tubes coupled to the insert tubes of the front support tubes coupled through the coupling holes , assembling holes formed at a set angle on the inner sides of the coupling holes on both sides of the support tube such that insert tubes of rear support tubes are penetrated and coupled to the backrest tubes at predetermined interval, insertion holes formed at a set angle on the inner sides of the assembling holes on both sides of the support tube such that insert tubes of base support tubes are penetrated and coupled to the assembling holes at predetermined intervals, and a base of which insertion parts are coupled to the base support tubes coupled through the insertion holes of both sides and to the backrest tubes coupled through the coupling holes of both sides. coupling holes formed at a set angle on both sides of a support tube such that insert tubes of front support tubes are penetrated and coupled through the coupling holes so as to be exposed, backrest tubes coupled to the insert tubes of the front support tubes coupled through the coupling holes , assembling holes formed at a set angle on the inner sides of the coupling holes on both sides of the support tube such that insert tubes of rear support tubes are penetrated and coupled to the backrest tubes at predetermined interval, insertion holes formed at a set angle on the inner sides of the assembling holes on both sides of the support tube such that insert tubes of base support tubes are penetrated and coupled to the assembling holes at predetermined intervals, and a base of which insertion parts are coupled to the base support tubes coupled through the insertion holes of both sides and to the backrest tubes coupled through the coupling holes of both sides.