A light sheet fluorescence microscope , comprising: a light source device configured to emit excitation light (E) suitable for inducing fluorescent light (F) emitted from a specimen , a detector device configured to detect the fluorescent light (F) from the specimen , and an optical system configured to illuminate the specimen with a light sheet (LS) formed from the excitation light (E) and to guide the fluorescent light (F) from the illuminated specimen to the detector device , wherein the optical system comprises an objective facing the specimen , the objective being configured to collect the fluorescent light (F) emitted from the specimen , wherein the optical system is configured to illuminate the specimen with the light sheet (LS) through the objective , and wherein the light source device is further configured to emit manipulation light (M) suitable for photomanipulating the specimen , wherein the optical system is further configured to direct the manipulation light (M) through the objective through an entrance pupil (EP) thereof onto the specimen along a light propagation direction which is different from a light propagation direction of the light sheet (LS), and wherein the optical system comprises a scanning mirror device including at least one tilting mirror which is tiltable for reflecting the excitation light (E) to move the light sheet (LS) as a whole through the specimen in a light sheet scanning direction transverse to the light propagation direction of the light sheet (LS), characterized in that the optical system is configured to direct the manipulation light (M) through a spatially limited sub-area of the entrance pupil (EP) of the objective on the specimen ; wherein the at least one scanning mirror is tiltable for reflecting both the excitation light (E) and the manipulation light (M). a light source device configured to emit excitation light (E) suitable for inducing fluorescent light (F) emitted from a specimen , a detector device configured to detect the fluorescent light (F) from the specimen , and an optical system configured to illuminate the specimen with a light sheet (LS) formed from the excitation light (E) and to guide the fluorescent light (F) from the illuminated specimen to the detector device , wherein the optical system comprises an objective facing the specimen , the objective being configured to collect the fluorescent light (F) emitted from the specimen , wherein the optical system is configured to illuminate the specimen with the light sheet (LS) through the objective , and wherein the light source device is further configured to emit manipulation light (M) suitable for photomanipulating the specimen , wherein the optical system is further configured to direct the manipulation light (M) through the objective through an entrance pupil (EP) thereof onto the specimen along a light propagation direction which is different from a light propagation direction of the light sheet (LS), and wherein the optical system comprises a scanning mirror device including at least one tilting mirror which is tiltable for reflecting the excitation light (E) to move the light sheet (LS) as a whole through the specimen in a light sheet scanning direction transverse to the light propagation direction of the light sheet (LS), characterized in that the optical system is configured to direct the manipulation light (M) through a spatially limited sub-area of the entrance pupil (EP) of the objective on the specimen ; wherein the at least one scanning mirror is tiltable for reflecting both the excitation light (E) and the manipulation light (M). <EOS>