An autofocusing system for a microscope , the autofocusing system being adapted to perform an autofocusing procedure , the autofocusing procedure comprising - predefining a set of target points for autofocusing, wherein at least one static rule (211-217) or at least one dynamic rule (211-217) exchangeable by a different rule (211-217) is associated to each of the predefined target points or to a subset thereof, - the at least one rule (211-217) defining at least one initial setting and at least one adaptation instruction for at least one parameter influencing how an autofocusing operation is performed for the target point , - the at least one adaptation instruction comprising at least one of an adaptation of the at least one parameter, an exchange of the at least one parameter, a substitution of the at least one rule (211-217) by at least one different rule, and a deactivation of the at least one rule (211-217), and - the at least one parameter including at least one of a position or position change of the target point , an activation or deactivation pattern of the autofocusing operation for the target point , a frequency in which the target point is subjected to the autofocusing operation , at least one light parameter used in the autofocusing operation , and the autofocusing method used in the autofocusing operation , - initiating for each of the predefined set of target points or a subset thereof an initial autofocusing operation comprising the at least one initial setting as defined by the at least one rule (211-217) associated to the target point , - initiating for each of the predefined set of target points or a subset thereof an adapted autofocusing operation based on the at least one adaptation instruction as defined by the at least one rule (211-217) associated to the target point , and - further processing an autofocus value for at least some of the target points , the autofocus value being obtained by the initial autofocusing operation or by the adapted autofocusing operation . - predefining a set of target points for autofocusing, wherein at least one static rule (211-217) or at least one dynamic rule (211-217) exchangeable by a different rule (211-217) is associated to each of the predefined target points or to a subset thereof, - the at least one rule (211-217) defining at least one initial setting and at least one adaptation instruction for at least one parameter influencing how an autofocusing operation is performed for the target point , - the at least one adaptation instruction comprising at least one of an adaptation of the at least one parameter, an exchange of the at least one parameter, a substitution of the at least one rule (211-217) by at least one different rule, and a deactivation of the at least one rule (211-217), and - the at least one parameter including at least one of a position or position change of the target point , an activation or deactivation pattern of the autofocusing operation for the target point , a frequency in which the target point is subjected to the autofocusing operation , at least one light parameter used in the autofocusing operation , and the autofocusing method used in the autofocusing operation , - initiating for each of the predefined set of target points or a subset thereof an initial autofocusing operation comprising the at least one initial setting as defined by the at least one rule (211-217) associated to the target point , - initiating for each of the predefined set of target points or a subset thereof an adapted autofocusing operation based on the at least one adaptation instruction as defined by the at least one rule (211-217) associated to the target point , and - further processing an autofocus value for at least some of the target points , the autofocus value being obtained by the initial autofocusing operation or by the adapted autofocusing operation . <EOS>