A system for forming a sterile fluid pathway comprising a first aseptic coupling device connected to a second aseptic coupling device , wherein: the first aseptic coupling device comprises an inner member defining a fluid passage therethrough, a first seal member coupled to a front surface of the inner member, a first membrane coupled to the front surface of the inner member to cover the first seal member, and a locking ring positioned to rotate about the inner member; the second aseptic coupling device comprises a main body defining a fluid passage therethrough, a front portion coupled to the main body, the front portion defining a plurality of channels therein, a second seal member coupled to a front surface of the main body, a second membrane coupled to the front surface of the main body to cover the second seal member, and a slot defined in the main body sized to receive a clip; wherein the inner member is sized to be received in the front portion of the second aseptic coupling device to form a pre-coupled state, wherein the front portion is sized to a receive the inner member of the first aseptic coupling device so that the clip engages the first aseptic coupling device to form the pre-coupled state; wherein, when the membrane is removed, the first and second seal members engage each other; and wherein, upon turning of the locking ring of the first aseptic coupling device, barbs of the locking ring are received within the channels of the front portion to compress the first seal member and the second seal member to form a coupled state in which a sterile flow path is created between the first aseptic device and the second aseptic device. the first aseptic coupling device comprises an inner member defining a fluid passage therethrough, a first seal member coupled to a front surface of the inner member, a first membrane coupled to the front surface of the inner member to cover the first seal member, and a locking ring positioned to rotate about the inner member; an inner member defining a fluid passage therethrough, a first seal member coupled to a front surface of the inner member, a first membrane coupled to the front surface of the inner member to cover the first seal member, and a locking ring positioned to rotate about the inner member; the second aseptic coupling device comprises a main body defining a fluid passage therethrough, a front portion coupled to the main body, the front portion defining a plurality of channels therein, a second seal member coupled to a front surface of the main body, a second membrane coupled to the front surface of the main body to cover the second seal member, and a slot defined in the main body sized to receive a clip; a main body defining a fluid passage therethrough, a front portion coupled to the main body, the front portion defining a plurality of channels therein, a second seal member coupled to a front surface of the main body, a second membrane coupled to the front surface of the main body to cover the second seal member, and a slot defined in the main body sized to receive a clip; wherein the inner member is sized to be received in the front portion of the second aseptic coupling device to form a pre-coupled state, wherein the front portion is sized to a receive the inner member of the first aseptic coupling device so that the clip engages the first aseptic coupling device to form the pre-coupled state; wherein, when the membrane is removed, the first and second seal members engage each other; and wherein, upon turning of the locking ring of the first aseptic coupling device, barbs of the locking ring are received within the channels of the front portion to compress the first seal member and the second seal member to form a coupled state in which a sterile flow path is created between the first aseptic device and the second aseptic device. <EOS>