mhurhangee/patent-ind-claim-en huggingface dataset:
A method of generating a library of variants of a sequence of interest, said method comprising the steps of: (a) providing a template DNA molecule comprising said sequence of interest; (b) providing a pair of oligonucleotides wherein said oligonucleotides hybridize to said sequence of interest, wherein one of said oligonucleotides is protected, the other oligonucleotide is non-protected, and said oligonucleotides flank said sequence of interest; (c) performing an amplification reaction to induce sequence changes relative to said template DNA molecule using said oligonucleotides, thereby generating a population of dsDNA variants; (d) incubating said population of dsDNA variants with an enzyme capable of selectively degrading the non-protected strand over the protected strand of said dsDNA variants, thereby producing a population of ssDNA variants; (e) hybridizing said population of ssDNA variants to ssDNA intermediaries, wherein said ssDNA intermediaries comprise a sequence substantially identical to said sequence of interest or a fragment thereof, generating heteroduplex DNA, wherein said ssDNA intermediaries are circular and are uracilated ssDNA intermediaries or methylated ssDNA intermediaries; and (f) transforming said heteroduplex DNA into cells, wherein said cells are capable of selectively degrading uracilated DNA or methylated DNA, thereby generating a library of variants of said sequence of interest. (a) providing a template DNA molecule comprising said sequence of interest; (b) providing a pair of oligonucleotides wherein said oligonucleotides hybridize to said sequence of interest, wherein one of said oligonucleotides is protected, the other oligonucleotide is non-protected, and said oligonucleotides flank said sequence of interest; (c) performing an amplification reaction to induce sequence changes relative to said template DNA molecule using said oligonucleotides, thereby generating a population of dsDNA variants; (d) incubating said population of dsDNA variants with an enzyme capable of selectively degrading the non-protected strand over the protected strand of said dsDNA variants, thereby producing a population of ssDNA variants; (e) hybridizing said population of ssDNA variants to ssDNA intermediaries, wherein said ssDNA intermediaries comprise a sequence substantially identical to said sequence of interest or a fragment thereof, generating heteroduplex DNA, wherein said ssDNA intermediaries are circular and are uracilated ssDNA intermediaries or methylated ssDNA intermediaries; and (f) transforming said heteroduplex DNA into cells, wherein said cells are capable of selectively degrading uracilated DNA or methylated DNA, thereby generating a library of variants of said sequence of interest.
Method for controlling a battery-operated welding device , the accumulator voltage (U in ) and the accumulator current (I in ) provided by an accumulator being regulated to a welding current (I out ) and welding voltage (U out ), supplied to a welding torch , by means of a welding controller that contains a step-up converter having at least one switch and a step-down converter having at least one switch , characterised in that the step-up converter is bridged with the aid of a switch controlled by a switch controller when the intermediate circuit voltage (U zw ) between the step-up converter and the step-down converter is less than or equal to the accumulator voltage (U in ), and the switch for bridging the step-up converter is opened when the step-up converter is activated and the welding current (I out ) is less than the maximum current (I b,max ) through the step-up converter , and the step-down converter is deactivated.
A method of managing a neighbor table in a communication apparatus , the method comprising the steps of the communication apparatus maintaining a neighbor table comprising a plurality of neighboring table entries corresponding to respective neighboring nodes being communication nodes (10a-j) in direct communication range of the communication apparatus , the method charaterized in that: said maintaining including, upon determination (SI03) that the neighbor table capacity has reached a threshold, replacing (SI07) a neighbor table entry corresponding to a neighboring node from the neighbor table by a new neighbor table entry corresponding to a new neighboring node based on the determination (SI05) that the new neighboring node is a communication partner, wherein a communication node (10a-j) is defined as a communication partner if at least one the following conditions is met: condition a a message including the communication partner as source address has been received by the communication apparatus , or condition b a message including the communication partner as destination address has been transmitted by the communication apparatus , or condition c the new neighboring node is defined as a communication partner by configuration at the communication apparatus , wherein the neighboring node is either not a communication partner of the communication apparatus or is a communication partner with a link quality between the communication apparatus and the neighboring node being lower than a link quality between the communication apparatus and the new neighboring node. condition a a message including the communication partner as source address has been received by the communication apparatus , or condition b a message including the communication partner as destination address has been transmitted by the communication apparatus , or condition c the new neighboring node is defined as a communication partner by configuration at the communication apparatus , wherein the neighboring node is either not a communication partner of the communication apparatus or is a communication partner with a link quality between the communication apparatus and the neighboring node being lower than a link quality between the communication apparatus and the new neighboring node.
Method for detecting a failure of an operation of an accelerator pedal (PA) of a vehicle (V) further comprising a brake pedal (PF) and a clutch pedal (PE), characterized in that it comprises a step in which, in the event of detection of simultaneous use of said brake pedal (PF) and accelerator pedal (PA), it is checked whether said clutch pedal (PE) is used , and in the negative one deduces from this check that said accelerator pedal (PA) is the subject of a malfunction, and operating a powertrain of said vehicle (V) in a degraded mode, said method being further characterized in that before operating said powertrain in degraded mode, a second check of the use of said is carried out clutch pedal (PE) so as to order the introduction of said degraded mode only if said clutch pedal (PE) is still not used.
A detection system for determining pyruvate dehydrogenase (PDH) levels in a bodily sample, comprising: at least one reaction solution for generating NAD + upon combination with PDH in the bodily sample, the reaction solution including pyruvate and NADH; and a biosensor for determining the level of generated NAD + , the biosensor including a working electrode formed on a surface of a substrate, a counter electrode formed on the surface of the substrate, and a measuring device for applying voltage potentials to the working electrode and counter electrode and measuring the current flow between the working electrode and counter electrode, wherein the reaction solution does not include additional reagents that contribute to background oxidation current of the biosensor and impair detection and quantification of the NAD + generated.
A method for manufacturing an oxymethylene copolymer, comprising: a step 1 of polymerizing a polymerization raw material containing trioxane and a comonomer in the presence of 1.0 × 10 -8 moles or more and 5.0 × 10 -6 moles or less of an acid catalyst per 1 mole of trioxane; a step 2 of adding and mixing a hydroxylamine compound represented by General formula below: where R 1 and R 2 each independently represent a hydrogen atom or a C1-C20 organic group, with the product obtained in the step 1, wherein the hydroxylamine compound is in an amount of 50-5000 molar times the amount of the acid catalyst; and a step 3 of further melt-kneading the mixture of the product and the hydroxylamine compound obtained in the step 2. a step 1 of polymerizing a polymerization raw material containing trioxane and a comonomer in the presence of 1.0 × 10 -8 moles or more and 5.0 × 10 -6 moles or less of an acid catalyst per 1 mole of trioxane; a step 2 of adding and mixing a hydroxylamine compound represented by General formula below: where R 1 and R 2 each independently represent a hydrogen atom or a C1-C20 organic group, with the product obtained in the step 1, wherein the hydroxylamine compound is in an amount of 50-5000 molar times the amount of the acid catalyst; and a step 3 of further melt-kneading the mixture of the product and the hydroxylamine compound obtained in the step 2.
A method for upgrading a software package performed by an apparatus for controlling a secure boot of a board, the method comprising: • obtaining a new software package of the board (S 201); • using a software package public key contained in the new software package to check an original signature of the new software package, wherein the original signature is contained in a secure world of a central processing unit on the board, wherein the secure world of the central processing unit is physically isolated from a normal world of the central processing unit (S 202); • after the original signature of the new software package passes the check, re-signing the new software package by using a board private key of the board in the secure world, thereby arriving at a re-signature of the new software package, wherein the re-signature is used to check security of the software package when the board boots (S 203); • storing the new software package and the re-signature of the new software package in the board, and subsequently replacing, in the board, a corresponding software package of an earlier version and a re-signature corresponding to the software package of the earlier version with the new software package and the re-signature of the new software package, wherein the software package of the earlier version comprises a software package public key of the earlier version. • obtaining a new software package of the board (S 201); • using a software package public key contained in the new software package to check an original signature of the new software package, wherein the original signature is contained in a secure world of a central processing unit on the board, wherein the secure world of the central processing unit is physically isolated from a normal world of the central processing unit (S 202); • after the original signature of the new software package passes the check, re-signing the new software package by using a board private key of the board in the secure world, thereby arriving at a re-signature of the new software package, wherein the re-signature is used to check security of the software package when the board boots (S 203); • storing the new software package and the re-signature of the new software package in the board, and subsequently replacing, in the board, a corresponding software package of an earlier version and a re-signature corresponding to the software package of the earlier version with the new software package and the re-signature of the new software package, wherein the software package of the earlier version comprises a software package public key of the earlier version.
Coulter arrangement for a seed drill, having - a sowing coulter for depositing seed, wherein the sowing coulter is fastened to a movable sowing coulter mount ; and - a fertilizer coulter for depositing fertilizer, wherein the fertilizer coulter is fastened to a movable support arm ; wherein the sowing coulter mount and the support arm are fastened to a common crossbeam ; and wherein the movement of the sowing coulter mount and the movement of the support arm are coupled together via a variable-length coupling device , characterized in that the variable-length coupling device comprises a spring element . - a sowing coulter for depositing seed, wherein the sowing coulter is fastened to a movable sowing coulter mount ; and - a fertilizer coulter for depositing fertilizer, wherein the fertilizer coulter is fastened to a movable support arm ;
A method comprising: forming an active layer for an integrated circuit chip , the active layer including an active device layer and a metal interconnect layer above an insulator layer , the active device layer including a gate region and a channel region of a transistor , wherein the active layer is formed in a semiconductor wafer ; forming a trap rich layer for the integrated circuit chip , wherein the trap rich layer is formed in a handle wafer above and after the formation of the active layer and degrades carrier lifetimes of charge carriers, wherein the gate region is between the channel region and the trap rich layer ; forming a bonding layer between the trap rich layer and the active layer , wherein the bonding layer is thicker than the insulator layer ; and bonding the handle wafer to the semiconductor wafer . forming an active layer for an integrated circuit chip , the active layer including an active device layer and a metal interconnect layer above an insulator layer , the active device layer including a gate region and a channel region of a transistor , wherein the active layer is formed in a semiconductor wafer ; forming a trap rich layer for the integrated circuit chip , wherein the trap rich layer is formed in a handle wafer above and after the formation of the active layer and degrades carrier lifetimes of charge carriers, wherein the gate region is between the channel region and the trap rich layer ; forming a bonding layer between the trap rich layer and the active layer , wherein the bonding layer is thicker than the insulator layer ; and bonding the handle wafer to the semiconductor wafer .
A surgical stapler comprising: a cartridge assembly having a proximal end and a distal end; and a jaw assembly at the distal end of elongate shaft assembly; the jaw assembly comprising: a first jaw having an anvil surface ; a second jaw ; the first jaw being movable relative to the second jaw and having a closed position in which the anvil surface is adjacent to the second jaw and a gap is defined between the second jaw and the anvil surface ; the second jaw comprising a staple cartridge receiving portion ; and a staple cartridge positionable in the staple cartridge receiving portion , characterized in that the staple cartridge comprises: a first plate , a second plate , and a third plate connected together, the first plate having an inner surface and an outer surface opposite the inner surface , the first plate comprising a plurality of staple holding locations formed therein, each staple holding location defined by a recess formed in the inner surface of the first plate ; and a plurality of staples positioned in the plurality of staple holding locations . a cartridge assembly having a proximal end and a distal end; and a jaw assembly at the distal end of elongate shaft assembly; a first jaw having an anvil surface ; a second jaw ; the first jaw being movable relative to the second jaw and having a closed position in which the anvil surface is adjacent to the second jaw and a gap is defined between the second jaw and the anvil surface ; the second jaw comprising a staple cartridge receiving portion ; and a staple cartridge positionable in the staple cartridge receiving portion , characterized in that the staple cartridge comprises: a first plate , a second plate , and a third plate connected together, the first plate having an inner surface and an outer surface opposite the inner surface , the first plate comprising a plurality of staple holding locations formed therein, each staple holding location defined by a recess formed in the inner surface of the first plate ; and a plurality of staples positioned in the plurality of staple holding locations . a first plate , a second plate , and a third plate connected together, the first plate having an inner surface and an outer surface opposite the inner surface , the first plate comprising a plurality of staple holding locations formed therein, each staple holding location defined by a recess formed in the inner surface of the first plate ; and a plurality of staples positioned in the plurality of staple holding locations .
A synchronizer device for a manual transmission, comprising a sliding sleeve which includes an internal toothing having a multitude of sliding sleeve teeth and is rotatable about a transmission axis (A), a clutch body of a speed change gear, the clutch body having an external toothing having a multitude of clutch body teeth for the internal toothing of the sliding sleeve to engage therein, and a synchronizer unit which is adapted to block an axial movement of the sliding sleeve , characterized in that the clutch body teeth at the axial tooth ends thereof adjacent to the sliding sleeve and/or at least some of the sliding sleeve teeth at the axial tooth ends thereof adjacent to the clutch body each include one single convex end face or each include a plurality of convex end faces, wherein the single convex end face or one of the plurality of convex end faces each extends in the circumferential direction from a first root point adjacent to a tooth flank of the respective tooth via an axially protruding apex portion as far as to a second root point , the second root point adjoining an opposite tooth flank of the tooth when there is one single convex end face and being a valley point of an indentation between neighboring convex end faces when there is a plurality of convex end faces, and wherein an axial distance (a) between the apex portion and each of the root points amounts to a maximum of 18%, in particular a maximum of 10%, of a tooth width (b) of the tooth .
An actuator comprising: an actuator body having a through hole that is formed in an axial direction and an output piston portion disposed so as to be slidable in the through hole , the actuator being configured for causing a predetermined force to act on an object by causing the output piston portion to protrude from an output surface of the actuator body , and the actuator further comprising: an ignition device for combusting gunpowder , the ignition device applying, to the output piston portion , driving energy for causing the output piston portion to slide using gunpowder combustion in the ignition device ; and a sealing member that is formed of a metal plate-like member, that separates a space in the actuator body into a first space in which the ignition device is disposed and a second space in which the output piston portion is disposed, and that seals a combustion product generated by the ignition device in the first space , wherein the sealing member is configured to be deformed by the gunpowder combustion in the ignition device such that a central portion of the sealing member is displaced to a side of the output piston portion , to thereby press the output piston portion using the central portion and cause the output piston portion to slide in the through hole , the ignition device includes a cup that defines a space for disposing gunpowder , and the central portion is positioned so as to face the cup , characterised in that a peripheral edge portion of the sealing member is fixed to an inner wall that defines the space in the actuator body an ignition device for combusting gunpowder , the ignition device applying, to the output piston portion , driving energy for causing the output piston portion to slide using gunpowder combustion in the ignition device ; and a sealing member that is formed of a metal plate-like member, that separates a space in the actuator body into a first space in which the ignition device is disposed and a second space in which the output piston portion is disposed, and that seals a combustion product generated by the ignition device in the first space , wherein the sealing member is configured to be deformed by the gunpowder combustion in the ignition device such that a central portion of the sealing member is displaced to a side of the output piston portion , to thereby press the output piston portion using the central portion and cause the output piston portion to slide in the through hole , the ignition device includes a cup that defines a space for disposing gunpowder , and the central portion is positioned so as to face the cup , characterised in that a peripheral edge portion of the sealing member is fixed to an inner wall that defines the space in the actuator body
A composite component comprising a plurality of layers , characterised in that the composite component further comprises: a fracture region including at least one fracture inducing layer (76; 76' 76") in overlapping relationship with at least one of the plurality of layers , wherein: at least one of the plurality of layers is configured to remain substantially intact when the at least one fracture inducing layer (76; 76' 76") fails; the fracture inducing layer (76; 76") includes a discontinuity at the fracture region , the discontinuity including a plurality of perforations in the at least one fracture inducing layer ; and the plurality of layers are configured to withstand a first loading and the at least one fracture inducing layer (76; 76' 76") is configured to withstand a second loading, the first loading is greater than the second loading. at least one of the plurality of layers is configured to remain substantially intact when the at least one fracture inducing layer (76; 76' 76") fails; the fracture inducing layer (76; 76") includes a discontinuity at the fracture region , the discontinuity including a plurality of perforations in the at least one fracture inducing layer ; and the plurality of layers are configured to withstand a first loading and the at least one fracture inducing layer (76; 76' 76") is configured to withstand a second loading, the first loading is greater than the second loading.
A live virus composition comprising a herpes simplex virus, a protein, at least one sugar, sodium chloride, and sodium phosphate, wherein the composition is at pH 7.4, wherein the composition is frozen, the protein is partially hydrolyzed gelatin at a concentration of 0.5% (w/v), and the at least one sugar is sorbitol at a concentration of 2% (w/v). wherein the composition is frozen, the protein is partially hydrolyzed gelatin at a concentration of 0.5% (w/v), and the at least one sugar is sorbitol at a concentration of 2% (w/v).