A method of using a nanopore sequencer, the nanopore sequencer comprising: a cis well ; a trans well ; a nanopore fluidically connecting the cis well and the trans well ; and a modified electrolyte in the trans well , wherein the modified electrolyte includes a potassium cation and an associated anion, a sodium cation and the associated anion, or combinations thereof; characterized in that the modified electrolyte further includes a cation complexing agent, the cation complexing agent is selected from the group consisting of a crown ether, a calixarene and valinomycin, wherein the crown ether is selected from 15-crown-5, 18-crown-6, 21-crown-7, benzo-15-crown-5, dibenzo-15-crown-5, benzo-18-crown-6, dibenzo-18-crown-6, benzo-21-crown-7, dibenzo-21-crown-7, dicyclohexano-18-crown-6, dicyclohexano-21-crown-7, azacrown and thiacrown; and wherein the cis well comprises the electrolyte without the cation complexing agent; the method comprising: controlling depletion of a redox reagent of the electrolyte in the nanopore sequencer by applying a voltage bias ranging from about -1 V to about 1 V between the cis well of the nanopore sequencer and the trans well of the nanopore sequencer . the nanopore sequencer comprising: a cis well ; a trans well ; a nanopore fluidically connecting the cis well and the trans well ; and a modified electrolyte in the trans well , wherein the modified electrolyte includes a potassium cation and an associated anion, a sodium cation and the associated anion, or combinations thereof; characterized in that the modified electrolyte further includes a cation complexing agent, the cation complexing agent is selected from the group consisting of a crown ether, a calixarene and valinomycin, wherein the crown ether is selected from 15-crown-5, 18-crown-6, 21-crown-7, benzo-15-crown-5, dibenzo-15-crown-5, benzo-18-crown-6, dibenzo-18-crown-6, benzo-21-crown-7, dibenzo-21-crown-7, dicyclohexano-18-crown-6, dicyclohexano-21-crown-7, azacrown and thiacrown; and wherein the cis well comprises the electrolyte without the cation complexing agent; a cis well ; a trans well ; a nanopore fluidically connecting the cis well and the trans well ; and a modified electrolyte in the trans well , wherein the modified electrolyte includes a potassium cation and an associated anion, a sodium cation and the associated anion, or combinations thereof; characterized in that the modified electrolyte further includes a cation complexing agent, the cation complexing agent is selected from the group consisting of a crown ether, a calixarene and valinomycin, wherein the crown ether is selected from 15-crown-5, 18-crown-6, 21-crown-7, benzo-15-crown-5, dibenzo-15-crown-5, benzo-18-crown-6, dibenzo-18-crown-6, benzo-21-crown-7, dibenzo-21-crown-7, dicyclohexano-18-crown-6, dicyclohexano-21-crown-7, azacrown and thiacrown; and wherein the cis well comprises the electrolyte without the cation complexing agent; the method comprising: controlling depletion of a redox reagent of the electrolyte in the nanopore sequencer by applying a voltage bias ranging from about -1 V to about 1 V between the cis well of the nanopore sequencer and the trans well of the nanopore sequencer . <EOS>