A chromatographic device for separating analytes in a sample comprising: a sample injector having a sample injection needle for injecting the sample into the mobile phase; a sample reservoir container in fluid communication with the sample injector; a chromatography column downstream of the sample injector, the chromatography column having fluid connectors; and fluid conduits connecting the sample injector and the chromatography column; wherein interior surfaces of the fluid conduits, sample injector, sample reservoir container, and chromatography column form a fluidic flow path having wetted surfaces; and wherein at least a portion of the wetted surfaces of the fluidic flow path are coated with an alkylsilyl coating having the Formula I: wherein each X is independently selected from (C 1 -C 6 )alkoxy, -NH(Ci-C 6 )alkyl, -N((C 1 -C 6 )alkyl) 2 , OH, OR A , R B , R C , R D and halo; R A represents a point of attachment to the interior surfaces of the fluidic system and at least one X is OR A ; R B is absent or represents a hydrophobicity modifier; R C represents a charge modifier and at least one X is R C ; R D is absent, a chelator, or a crown ether; and Y is a bridging moiety selected from (C 1 -C 20 )alkyl, -O[(CH 2 ) 2 O] 1-20 -, - (C 1 -C 10 )[NH(CO)NH(C 1 -C 10 )] 1-20 -, or -(C 1 -C 10 )[alkylphenyl(C 1 -C 10 )alkyl] 1-20 -. a sample injector having a sample injection needle for injecting the sample into the mobile phase; a sample reservoir container in fluid communication with the sample injector; a chromatography column downstream of the sample injector, the chromatography column having fluid connectors; and fluid conduits connecting the sample injector and the chromatography column; wherein interior surfaces of the fluid conduits, sample injector, sample reservoir container, and chromatography column form a fluidic flow path having wetted surfaces; and wherein at least a portion of the wetted surfaces of the fluidic flow path are coated with an alkylsilyl coating having the Formula I: wherein each X is independently selected from (C 1 -C 6 )alkoxy, -NH(Ci-C 6 )alkyl, -N((C 1 -C 6 )alkyl) 2 , OH, OR A , R B , R C , R D and halo; R A represents a point of attachment to the interior surfaces of the fluidic system and at least one X is OR A ; R B is absent or represents a hydrophobicity modifier; R C represents a charge modifier and at least one X is R C ; R D is absent, a chelator, or a crown ether; and Y is a bridging moiety selected from (C 1 -C 20 )alkyl, -O[(CH 2 ) 2 O] 1-20 -, - (C 1 -C 10 )[NH(CO)NH(C 1 -C 10 )] 1-20 -, or -(C 1 -C 10 )[alkylphenyl(C 1 -C 10 )alkyl] 1-20 -. wherein each X is independently selected from (C 1 -C 6 )alkoxy, -NH(Ci-C 6 )alkyl, -N((C 1 -C 6 )alkyl) 2 , OH, OR A , R B , R C , R D and halo; R A represents a point of attachment to the interior surfaces of the fluidic system and at least one X is OR A ; R B is absent or represents a hydrophobicity modifier; R C represents a charge modifier and at least one X is R C ; R D is absent, a chelator, or a crown ether; and Y is a bridging moiety selected from (C 1 -C 20 )alkyl, -O[(CH 2 ) 2 O] 1-20 -, - (C 1 -C 10 )[NH(CO)NH(C 1 -C 10 )] 1-20 -, or -(C 1 -C 10 )[alkylphenyl(C 1 -C 10 )alkyl] 1-20 -. <EOS>