Method for quantifying the activity of the proteins or enzymes involved in the conjugation of the SUMO/Ubiquitin/Nedd8 proteins in a cell of a biological sample, said method comprising: a) a step of contacting a cellular extract of a cell with each protein of a subgroup of at least 3 proteins chosen among a group of 10 proteins, said group of 10 proteins belonging to a set of 46 proteins; said at least 3 proteins being immobilised on a support, said at least 3 proteins being substantially not conjugated by SUMO/ Ubiquitin/Nedd8 proteins before their contact with said cellular extract, wherein said at least 3 proteins corresponds to the proteins consisting essentially or consisting of the sequences SEQ ID NO: 1 to 3, wherein said 10 proteins corresponds to the proteins consisting essentially or consisting of the sequences SEQ ID NO: 1 to 10, and wherein said group of 46 proteins corresponds to the proteins consisting essentially or consisting of the sequences SEQ ID NO: 1 to 46, wherein SEQ ID NO: 1 corresponds to ZMYM5 protein; SEQ ID NO: 2 corresponds to BEAN protein and SEQ ID NO: 3 corresponds to OTUD6B, and b) a step of simultaneously measuring ubiquitination, SUMOylation and NEDDylation level of each of said at least 3 proteins to obtain a first value for ubiquitin, SUMO and Nedd8. a) a step of contacting a cellular extract of a cell with each protein of a subgroup of at least 3 proteins chosen among a group of 10 proteins, said group of 10 proteins belonging to a set of 46 proteins; said at least 3 proteins being immobilised on a support, said at least 3 proteins being substantially not conjugated by SUMO/ Ubiquitin/Nedd8 proteins before their contact with said cellular extract, wherein said at least 3 proteins corresponds to the proteins consisting essentially or consisting of the sequences SEQ ID NO: 1 to 3, wherein said 10 proteins corresponds to the proteins consisting essentially or consisting of the sequences SEQ ID NO: 1 to 10, and wherein said group of 46 proteins corresponds to the proteins consisting essentially or consisting of the sequences SEQ ID NO: 1 to 46, wherein SEQ ID NO: 1 corresponds to ZMYM5 protein; SEQ ID NO: 2 corresponds to BEAN protein and SEQ ID NO: 3 corresponds to OTUD6B, and said group of 10 proteins belonging to a set of 46 proteins; said at least 3 proteins being immobilised on a support, said at least 3 proteins being substantially not conjugated by SUMO/ Ubiquitin/Nedd8 proteins before their contact with said cellular extract, wherein said at least 3 proteins corresponds to the proteins consisting essentially or consisting of the sequences SEQ ID NO: 1 to 3, wherein said 10 proteins corresponds to the proteins consisting essentially or consisting of the sequences SEQ ID NO: 1 to 10, and wherein said group of 46 proteins corresponds to the proteins consisting essentially or consisting of the sequences SEQ ID NO: 1 to 46, wherein SEQ ID NO: 1 corresponds to ZMYM5 protein; SEQ ID NO: 2 corresponds to BEAN protein and SEQ ID NO: 3 corresponds to OTUD6B, and b) a step of simultaneously measuring ubiquitination, SUMOylation and NEDDylation level of each of said at least 3 proteins to obtain a first value for ubiquitin, SUMO and Nedd8. <EOS>