A three-dimensional bioprinter comprising: a case in which a working space is provided; a stage unit provided with a printing plate installed in the case and configured to slidingly move in vertical and lateral directions; a first nozzle installed in the case and configured to discharge a solid state biomaterial to the printing plate using a thermoplastic resin extrusion lamination method; a second nozzle installed in the case and configured to discharge a fluid state biomaterial to the printing plate ; a controller configured to control discharge of the first nozzle and the second nozzle , wherein the first nozzle and the second nozzle are used to stack the solid state biomaterial and the fluid state biomaterial to be printed in one structure; and a temperature adjusting part connected to the case to adjust a temperature of at least one of the printing plate and the second nozzle , wherein the controller controls the temperature adjusting part , characterized by the controller is further configured to independently control the temperature of each of the printing plate and the second nozzle through the temperature adjusting part and to control the second nozzle to gradationally discharge the fluid state biomaterial; wherein the gradational discharge is performed by a step motor connected to the second nozzle , the 3D bioprinter comprising an insulation cover configured to surround an exterior of the second nozzle and insulate the second nozzle , and in that the temperature adjusting part includes: a water tank configured to store cooling water; a Peltier member in which, when a temperature of one surface rises, a temperature of the other surface falls; and a heating member configured to increase the temperature of each of the printing plate and the second nozzle . a case in which a working space is provided; a stage unit provided with a printing plate installed in the case and configured to slidingly move in vertical and lateral directions; a first nozzle installed in the case and configured to discharge a solid state biomaterial to the printing plate using a thermoplastic resin extrusion lamination method; a second nozzle installed in the case and configured to discharge a fluid state biomaterial to the printing plate ; a controller configured to control discharge of the first nozzle and the second nozzle , wherein the first nozzle and the second nozzle are used to stack the solid state biomaterial and the fluid state biomaterial to be printed in one structure; and a temperature adjusting part connected to the case to adjust a temperature of at least one of the printing plate and the second nozzle , wherein the controller controls the temperature adjusting part , characterized by the controller is further configured to independently control the temperature of each of the printing plate and the second nozzle through the temperature adjusting part and to control the second nozzle to gradationally discharge the fluid state biomaterial; wherein the gradational discharge is performed by a step motor connected to the second nozzle , the 3D bioprinter comprising an insulation cover configured to surround an exterior of the second nozzle and insulate the second nozzle , and in that the temperature adjusting part includes: a water tank configured to store cooling water; a Peltier member in which, when a temperature of one surface rises, a temperature of the other surface falls; and a heating member configured to increase the temperature of each of the printing plate and the second nozzle . a water tank configured to store cooling water; a Peltier member in which, when a temperature of one surface rises, a temperature of the other surface falls; and a heating member configured to increase the temperature of each of the printing plate and the second nozzle . <EOS>