A method for manufacturing a cell suspension, the method comprising (A), (B) and (C) described below: (A) culturing adherent cells in a cell suspension containing the adherent cells, a microcarrier and a medium, and having a volume of at least 0.3 L, (B) culturing the adherent cells in a cell suspension containing the adherent cells obtained through (A), a microcarrier and a medium, and having a volume of at least 5 L, and (C) culturing the adherent cells in a cell suspension containing the adherent cells obtained through (B), a microcarrier and a medium, and having a volume of at least 10 L, wherein a volume of the cell suspension in (B) is greater than a volume of the cell suspension in (A), and a volume of the cell suspension in (C) is greater than a volume of the cell suspension in (B), characterized in that at least one selected from the group consisting of (A), (B) and (C) includes conducting intermittent agitation of the cell suspension, the intermittent agitation refers to alternately conducting agitation for a prescribed period and then not conducting agitation for a prescribed period, the time for which agitation is conducted is within a range from 0.5 to 60 minutes, and the time for which agitation is not conducted is within a range from 0.1 to 10 hours, (A) includes obtaining a cell suspension containing the adherent cells, a fresh microcarrier and a medium and having a volume of at least 0.3 L, and culturing the adherent cells, (B) includes obtaining a cell suspension containing the adherent cells obtained through (A), a fresh microcarrier and a medium and having a volume of at least 5 L, and culturing the adherent cells, and (C) includes obtaining a cell suspension containing the adherent cells obtained through (B), a fresh microcarrier and a medium and having a volume of at least 10 L, and culturing the adherent cells. (A) culturing adherent cells in a cell suspension containing the adherent cells, a microcarrier and a medium, and having a volume of at least 0.3 L, (B) culturing the adherent cells in a cell suspension containing the adherent cells obtained through (A), a microcarrier and a medium, and having a volume of at least 5 L, and (C) culturing the adherent cells in a cell suspension containing the adherent cells obtained through (B), a microcarrier and a medium, and having a volume of at least 10 L, wherein a volume of the cell suspension in (B) is greater than a volume of the cell suspension in (A), and a volume of the cell suspension in (C) is greater than a volume of the cell suspension in (B), characterized in that at least one selected from the group consisting of (A), (B) and (C) includes conducting intermittent agitation of the cell suspension, the intermittent agitation refers to alternately conducting agitation for a prescribed period and then not conducting agitation for a prescribed period, the time for which agitation is conducted is within a range from 0.5 to 60 minutes, and the time for which agitation is not conducted is within a range from 0.1 to 10 hours, (A) includes obtaining a cell suspension containing the adherent cells, a fresh microcarrier and a medium and having a volume of at least 0.3 L, and culturing the adherent cells, (B) includes obtaining a cell suspension containing the adherent cells obtained through (A), a fresh microcarrier and a medium and having a volume of at least 5 L, and culturing the adherent cells, and (C) includes obtaining a cell suspension containing the adherent cells obtained through (B), a fresh microcarrier and a medium and having a volume of at least 10 L, and culturing the adherent cells. <EOS>