An electrochemical analyte sensor comprising: a base layer; a working electrode disposed on the base layer and a multilayer analyte sensor stack disposed upon the working electrode comprising: (a) an analyte sensing layer disposed directly on the working electrode, wherein the analyte sensing layer detectable alters the electrical current at the working electrode in the presence of an analyte, wherein the analyte sensing layer comprises glucose oxidase disposed in the layer so that the analyte sensor senses glucose; (b) a high-density amine layer disposed over the analyte sensing layer, wherein the high-density amine layer comprises polymers having repeating amine groups; and (c) an analyte modulating layer disposed over the high-density amine layer, wherein the analyte modulating layer modulates the diffusion of analyte from an external environment to the working electrode, wherein the high-density amine layer comprises a first side in direct contact with the analyte sensing layer, and a second side in direct contact with the analyte modulating layer and functions as an adhesive layer that binds the analyte sensing layer to the analyte modulating layer, and wherein the analyte modulating layer is a glucose limiting membrane that comprises a polyurethane/polyurea polymer formed from a mixture comprising a diisocyanate, a hydrophilic polymer comprising a hydrophilic diol or hydrophilic diamine, and a siloxane having an amino, hydroxyl or carboxylic acid functional group at a terminus. a base layer; a working electrode disposed on the base layer and a multilayer analyte sensor stack disposed upon the working electrode comprising: (a) an analyte sensing layer disposed directly on the working electrode, wherein the analyte sensing layer detectable alters the electrical current at the working electrode in the presence of an analyte, wherein the analyte sensing layer comprises glucose oxidase disposed in the layer so that the analyte sensor senses glucose; (b) a high-density amine layer disposed over the analyte sensing layer, wherein the high-density amine layer comprises polymers having repeating amine groups; and (c) an analyte modulating layer disposed over the high-density amine layer, wherein the analyte modulating layer modulates the diffusion of analyte from an external environment to the working electrode, (a) an analyte sensing layer disposed directly on the working electrode, wherein the analyte sensing layer detectable alters the electrical current at the working electrode in the presence of an analyte, wherein the analyte sensing layer comprises glucose oxidase disposed in the layer so that the analyte sensor senses glucose; (b) a high-density amine layer disposed over the analyte sensing layer, wherein the high-density amine layer comprises polymers having repeating amine groups; and (c) an analyte modulating layer disposed over the high-density amine layer, wherein the analyte modulating layer modulates the diffusion of analyte from an external environment to the working electrode, wherein the high-density amine layer comprises a first side in direct contact with the analyte sensing layer, and a second side in direct contact with the analyte modulating layer and functions as an adhesive layer that binds the analyte sensing layer to the analyte modulating layer, and wherein the analyte modulating layer is a glucose limiting membrane that comprises a polyurethane/polyurea polymer formed from a mixture comprising a diisocyanate, a hydrophilic polymer comprising a hydrophilic diol or hydrophilic diamine, and a siloxane having an amino, hydroxyl or carboxylic acid functional group at a terminus. <EOS>