A method for producing a human cell aggregate comprising adenohypophysis or a precursor tissue thereof, which comprises the steps of: rapidly aggregating dispersed human pluripotent stem cells to form one aggregate in one culture compartment and culturing the aggregate of human pluripotent stem cells in suspension in a serum-free medium supplemented with a bone morphogenetic protein signal transduction pathway activating substance and a Shh signal pathway enhancer to obtain a human cell aggregate comprising a hypothalamus neuroepithelial tissue and a surface ectoderm that may contain a thickened epidermal placode in a part thereof; and further culturing the obtained human cell aggregate comprising the hypothalamus neuroepithelial tissue and the surface ectoderm in suspension in a serum-free medium supplemented with a bone morphogenetic protein signal transduction pathway activating substance and a Shh signal pathway enhancer to induce formation of hypophysial placode and/or Rathke's pouch in the surface ectoderm, thereby obtaining a human cell aggregate comprising i) hypothalamus neuroepithelial tissue, and ii) hypophysial placode and/or Rathke's pouch; and culturing the obtained human cell aggregate comprising i) hypothalamus neuroepithelial tissue, and ii) hypophysial placode and/or Rathke's pouch in suspension in a serum-free medium comprising a Shh signal pathway enhancer to induce differentiation of hypophysial placode and/or Rathke's pouch into pituitary hormone-producing cells, thereby obtaining a human cell aggregate comprising adenohypophysis; wherein the bone morphogenetic protein signal transduction pathway activating substance is selected from the group consisting of BMP2, BMP4, BMP7 and GDF5; and wherein the Shh signal pathway enhancer is selected from the group consisting of proteins belonging to the Hedgehog family, Shh, Shh receptor, Shh receptor agonist, Purmorphamine and Smoothened Agonist (SAG) (3-Chloro-N-[trans-4-(methylamino)cyclohexyl]-N-[[3-(4-pyridinyl)phenyl]methyl]- benzo[b]thiophene-2-carboxamide). rapidly aggregating dispersed human pluripotent stem cells to form one aggregate in one culture compartment and culturing the aggregate of human pluripotent stem cells in suspension in a serum-free medium supplemented with a bone morphogenetic protein signal transduction pathway activating substance and a Shh signal pathway enhancer to obtain a human cell aggregate comprising a hypothalamus neuroepithelial tissue and a surface ectoderm that may contain a thickened epidermal placode in a part thereof; and further culturing the obtained human cell aggregate comprising the hypothalamus neuroepithelial tissue and the surface ectoderm in suspension in a serum-free medium supplemented with a bone morphogenetic protein signal transduction pathway activating substance and a Shh signal pathway enhancer to induce formation of hypophysial placode and/or Rathke's pouch in the surface ectoderm, thereby obtaining a human cell aggregate comprising i) hypothalamus neuroepithelial tissue, and ii) hypophysial placode and/or Rathke's pouch; and culturing the obtained human cell aggregate comprising i) hypothalamus neuroepithelial tissue, and ii) hypophysial placode and/or Rathke's pouch in suspension in a serum-free medium comprising a Shh signal pathway enhancer to induce differentiation of hypophysial placode and/or Rathke's pouch into pituitary hormone-producing cells, thereby obtaining a human cell aggregate comprising adenohypophysis; <EOS>