Method for counting moving particles , the particles being borne by a fluid , the fluid flowing through a fluidic chamber , the particles being entrained by the movement of the fluid, the method comprising the following steps: a) placing the fluidic chamber between a light source and an image sensor , the image sensor lying in a detection plane ( P 0 ); b) illuminating the fluidic chamber with the light source, the light source emitting an incident light wave that propagates along a propagation axis (Z), and acquiring, with the image sensor , a first image ( I , I ( t 1 )) representative of an exposure wave to which the image sensor is exposed, the image sensor comprising various pixels, each pixel being associated with a radial coordinate (x,y) in the detection plane ( P 0 ); c) on the basis of the acquired first image, obtaining three-dimensional coordinates ((x,y,z)(t 1 )) of particles, in the fluidic chamber, at a first time ( t 1 ); d) obtaining three-dimensional coordinates (( x , y , z )( t 2 )) of particles in the fluidic chamber at a second time ( t 2 ), subsequent to the first time; e) on the basis of the coordinates of the particles obtained at the first time and at the second time, determining potential movements (Δ) of the particles between said times; characterized in that the method also comprises the following steps: f) taking into account a model ( mod ) of the movement of the fluid in the fluidic chamber; g) on the basis of the model of the movement of the fluid considered in step f), validating movements among the potential movements (Δ) calculated in step e); h) on the basis of the movements (Δ v ) validated in step g), determining a number ( N ) of particles and/or coordinates of the particles at the first time and/or at the second time. a) placing the fluidic chamber between a light source and an image sensor , the image sensor lying in a detection plane ( P 0 ); b) illuminating the fluidic chamber with the light source, the light source emitting an incident light wave that propagates along a propagation axis (Z), and acquiring, with the image sensor , a first image ( I , I ( t 1 )) representative of an exposure wave to which the image sensor is exposed, the image sensor comprising various pixels, each pixel being associated with a radial coordinate (x,y) in the detection plane ( P 0 ); c) on the basis of the acquired first image, obtaining three-dimensional coordinates ((x,y,z)(t 1 )) of particles, in the fluidic chamber, at a first time ( t 1 ); d) obtaining three-dimensional coordinates (( x , y , z )( t 2 )) of particles in the fluidic chamber at a second time ( t 2 ), subsequent to the first time; e) on the basis of the coordinates of the particles obtained at the first time and at the second time, determining potential movements (Δ) of the particles between said times; f) taking into account a model ( mod ) of the movement of the fluid in the fluidic chamber; g) on the basis of the model of the movement of the fluid considered in step f), validating movements among the potential movements (Δ) calculated in step e); h) on the basis of the movements (Δ v ) validated in step g), determining a number ( N ) of particles and/or coordinates of the particles at the first time and/or at the second time. <EOS>