A microscopy method for imaging a specimen (S) comprising two alignment marks along a desired x'-direction of the specimen (S), the desired x'-direction defined by the alignment marks of the specimen (S), the specimen (S) being placed on an XY translation stage and movable by the XY translation stage so as to have portion thereof placed within the field of view of an image sensor , wherein the XY translation stage is movable in an x-direction and a y-direction to move the specimen (S) relative to the image sensor , the image sensor having a multitude of pixels arranged to define pixel rows and pixel columns, the desired x'-direction of the specimen (S) being angularly offset from the x-direction of the XY translation stage so as to define a slope and angle of offset relative thereto, the image sensor viewing only a discrete segment of the specimen (S) at a time, the method comprising the steps of: rotating the image sensor such that the pixel rows are substantially parallel with the desired x'-direction of the specimen (S), without rotating the XY translation stage ; determining the angle of offset of the desired x'-direction as compared to the x-direction of the XY translation stage ; establishing a first position for the specimen (S) relative to the image sensor as rotated in said step of rotating, said first position placing at least a portion of the specimen (S) within the field of view of the image sensor ; and, after said step of determining and said step of establishing, moving the specimen (S) with the XY translation stage to a second position along the desired x'-direction by moving the XY translation stage in the x-direction, wherein the second position places at least a second portion of the specimen (S) within the field of view of the image sensor , and the second position is not substantially shifted in a y'-direction of the specimen (S), the y'-direction being orthogonal to the x'-direction of the specimen, wherein said step of moving is based upon the angle of offset determined in said step of determining. rotating the image sensor such that the pixel rows are substantially parallel with the desired x'-direction of the specimen (S), without rotating the XY translation stage ; determining the angle of offset of the desired x'-direction as compared to the x-direction of the XY translation stage ; establishing a first position for the specimen (S) relative to the image sensor as rotated in said step of rotating, said first position placing at least a portion of the specimen (S) within the field of view of the image sensor ; and, after said step of determining and said step of establishing, moving the specimen (S) with the XY translation stage to a second position along the desired x'-direction by moving the XY translation stage in the x-direction, wherein the second position places at least a second portion of the specimen (S) within the field of view of the image sensor , and the second position is not substantially shifted in a y'-direction of the specimen (S), the y'-direction being orthogonal to the x'-direction of the specimen, wherein said step of moving is based upon the angle of offset determined in said step of determining. <EOS>