A biological fluid collection device , comprising: a housing having a receiving port and an inlet port , the inlet port defining a housing flow channel therein, the housing flow channel in fluid communication with the inlet port ; a puncturing element at least partially disposed within the housing and transitionable between a pre-actuated position wherein the puncturing element is retained within the housing and a puncturing position wherein at least a portion of the puncturing element extends through the inlet port of the housing for establishing fluid communication with the housing flow channel ; and a cartridge removably connectable to the receiving port of the housing, the cartridge defining a cartridge flow channel therein, and wherein, with the cartridge connected to the housing , the cartridge flow channel is in fluid communication with the housing flow channel , wherein, with the cartridge disconnected from the housing , the cartridge flow channel is sealed, characterized in that the housing flow channel is adapted to receive a multicomponent blood sample having a cellular portion and a plasma portion , the cartridge includes a cartridge inlet port in fluid communication with the housing flow channel when the cartridge is received within the receiving port of the housing and a transfer port in fluid communication with the cartridge flow channel , and the cartridge includes an actuation member in fluid transfer communication with the transfer port , the actuation member transitionable between an initial position in which a multi-component blood sample is stored within the cartridge and an activated position in which at least a portion of the multi-component blood sample is expelled from the transfer port . a housing having a receiving port and an inlet port , the inlet port defining a housing flow channel therein, the housing flow channel in fluid communication with the inlet port ; a puncturing element at least partially disposed within the housing and transitionable between a pre-actuated position wherein the puncturing element is retained within the housing and a puncturing position wherein at least a portion of the puncturing element extends through the inlet port of the housing for establishing fluid communication with the housing flow channel ; and a cartridge removably connectable to the receiving port of the housing, the cartridge defining a cartridge flow channel therein, and wherein, with the cartridge connected to the housing , the cartridge flow channel is in fluid communication with the housing flow channel , wherein, with the cartridge disconnected from the housing , the cartridge flow channel is sealed, characterized in that the housing flow channel is adapted to receive a multicomponent blood sample having a cellular portion and a plasma portion , the cartridge includes a cartridge inlet port in fluid communication with the housing flow channel when the cartridge is received within the receiving port of the housing and a transfer port in fluid communication with the cartridge flow channel , and the cartridge includes an actuation member in fluid transfer communication with the transfer port , the actuation member transitionable between an initial position in which a multi-component blood sample is stored within the cartridge and an activated position in which at least a portion of the multi-component blood sample is expelled from the transfer port . <EOS>