A reaction device , comprising: a rotating disk configured to incubate, wash, and measure a reactant mixed with a sample in a reactor ; a washing assembly located above the rotating disk , and capable of dispensing a washing solution into the reactor and drawing unbound ingredients in the reactor , and the washing assembly comprising a signal reagent dispensing portion configured to dispense a signal reagent into the reactor subjected to a BF operation; a measuring device connected to the rotating disk , and configured to measure an optical signal of the reactant in the reactor after adding the signal reagent; and a transferring assembly configured to transfer the reactor ; wherein the rotating disk comprises an incubation ring , a washing ring , and a measuring ring that are arranged around a rotation center thereof, the incubation ring is provided with incubation positions that are arranged at intervals along a circumferential direction thereof and configured to incubate the reactant in the reactor , the washing ring is provided with washing positions that are arranged at intervals along a circumferential direction thereof and configured to wash the reactant in the reactor , the measuring ring is provided with measuring positions that are arranged at intervals along a circumferential direction thereof and configured to measure a signal of the reactant in the reactor , characterized in that the device further comprises: - a signal reagent mixing unit configured to mix the signal reagent in the reactor after adding the signal reagent; and wherein the transferring assembly is configured to transfer the reactor between the rotating disc and the signal reagent mixing unit , and to transfer the reactor between the signal reagent mixing unit , the incubation position , the washing position , and the measuring position , wherein a moving trajectory of the transferring assembly is on the same straight line. a rotating disk configured to incubate, wash, and measure a reactant mixed with a sample in a reactor ; a washing assembly located above the rotating disk , and capable of dispensing a washing solution into the reactor and drawing unbound ingredients in the reactor , and the washing assembly comprising a signal reagent dispensing portion configured to dispense a signal reagent into the reactor subjected to a BF operation; a measuring device connected to the rotating disk , and configured to measure an optical signal of the reactant in the reactor after adding the signal reagent; and a transferring assembly configured to transfer the reactor ; wherein the rotating disk comprises an incubation ring , a washing ring , and a measuring ring that are arranged around a rotation center thereof, the incubation ring is provided with incubation positions that are arranged at intervals along a circumferential direction thereof and configured to incubate the reactant in the reactor , the washing ring is provided with washing positions that are arranged at intervals along a circumferential direction thereof and configured to wash the reactant in the reactor , the measuring ring is provided with measuring positions that are arranged at intervals along a circumferential direction thereof and configured to measure a signal of the reactant in the reactor , characterized in that the device further comprises: - a signal reagent mixing unit configured to mix the signal reagent in the reactor after adding the signal reagent; - a signal reagent mixing unit configured to mix the signal reagent in the reactor after adding the signal reagent; and wherein the transferring assembly is configured to transfer the reactor between the rotating disc and the signal reagent mixing unit , and to transfer the reactor between the signal reagent mixing unit , the incubation position , the washing position , and the measuring position , wherein a moving trajectory of the transferring assembly is on the same straight line. <EOS>