Method for electrophoretic separation using a separation gel arrangement with a gel member and one or more sample wells for receiving sample liquid to be separated, the sample wells being in fluidic contact with the gel member, the method comprising the steps: adding a pre-labelled sample liquid to be separated in one or more of the sample wells, applying an electric field over the gel member to drive an electrophoretic separation process, whereby sample constituents are drawn from the sample liquid in the sample well(s) into the gel member for separation, monitoring the progress of the sample loading by optical detection of the pre-labelled sample, and when a removal criteria is met: discontinuing loading of sample constituents into the separation gel, wherein the removal criteria is determined based on the optical detection, the method being chartacterised in that the removal criteria is determined as a predefined loading ratio as optically detected, in turn determined by the steps of measuring the total label intensity in the sample well at start of the electrophoretic separation process, registering the label intensity in the sample well during the electrophoretic separation process, or registering the label intensity from the sample constituents drawn from the sample liquid in the sample well(s) into the gel member during the electrophoretic separation process, and calculating the loading ratio from the above intensities. adding a pre-labelled sample liquid to be separated in one or more of the sample wells, applying an electric field over the gel member to drive an electrophoretic separation process, whereby sample constituents are drawn from the sample liquid in the sample well(s) into the gel member for separation, monitoring the progress of the sample loading by optical detection of the pre-labelled sample, and when a removal criteria is met: discontinuing loading of sample constituents into the separation gel, wherein the removal criteria is determined based on the optical detection, the method being chartacterised in that the removal criteria is determined as a predefined loading ratio as optically detected, in turn determined by the steps of measuring the total label intensity in the sample well at start of the electrophoretic separation process, registering the label intensity in the sample well during the electrophoretic separation process, or registering the label intensity from the sample constituents drawn from the sample liquid in the sample well(s) into the gel member during the electrophoretic separation process, and calculating the loading ratio from the above intensities. monitoring the progress of the sample loading by optical detection of the pre-labelled sample, and when a removal criteria is met: discontinuing loading of sample constituents into the separation gel, wherein the removal criteria is determined based on the optical detection, the method being chartacterised in that the removal criteria is determined as a predefined loading ratio as optically detected, in turn determined by the steps of measuring the total label intensity in the sample well at start of the electrophoretic separation process, registering the label intensity in the sample well during the electrophoretic separation process, or registering the label intensity from the sample constituents drawn from the sample liquid in the sample well(s) into the gel member during the electrophoretic separation process, and calculating the loading ratio from the above intensities. <EOS>