A sterile fluid pathway assembly comprising: a sterile fluid container ; a sterile fluid conduit ; and a sterile fluid connector comprising a first portion configured to connect the sterile fluid conduit, and a second portion comprising a housing configured to mount the sterile fluid container; a connector hub ; a pierceable seal disposed at least partially between the connector hub and a sterile fluid chamber of the sterile fluid container, the pierceable seal in sealing contact with the connector hub to form a sterile cavity between the connector hub and the pierceable seal, wherein the sterile cavity is formed at least partially internal to the sterile fluid container; and a piercing member fixedly disposed within the connector hub capable of providing a sterile fluid communication between the sterile fluid conduit and the sterile fluid chamber of the sterile fluid container, the piercing member being stationary; wherein at least a portion of the pierceable seal is configured to transform from a non-activated state wherein the pierceable seal is intact, to an activated state wherein the pierceable seal is disrupted by the piercing member to create a sterile fluid communication between the sterile fluid conduit and the sterile fluid chamber of the sterile fluid container; and wherein the sterile fluid container comprises a plunger seal and translation of the plunger seal results in relative motion of the pierceable seal towards the piercing member. a sterile fluid container ; a sterile fluid conduit ; and a sterile fluid connector comprising a first portion configured to connect the sterile fluid conduit, and a second portion comprising a housing configured to mount the sterile fluid container; a connector hub ; a pierceable seal disposed at least partially between the connector hub and a sterile fluid chamber of the sterile fluid container, the pierceable seal in sealing contact with the connector hub to form a sterile cavity between the connector hub and the pierceable seal, wherein the sterile cavity is formed at least partially internal to the sterile fluid container; and a piercing member fixedly disposed within the connector hub capable of providing a sterile fluid communication between the sterile fluid conduit and the sterile fluid chamber of the sterile fluid container, the piercing member being stationary; a first portion configured to connect the sterile fluid conduit, and a second portion comprising a housing configured to mount the sterile fluid container; a connector hub ; a pierceable seal disposed at least partially between the connector hub and a sterile fluid chamber of the sterile fluid container, the pierceable seal in sealing contact with the connector hub to form a sterile cavity between the connector hub and the pierceable seal, wherein the sterile cavity is formed at least partially internal to the sterile fluid container; and a piercing member fixedly disposed within the connector hub capable of providing a sterile fluid communication between the sterile fluid conduit and the sterile fluid chamber of the sterile fluid container, the piercing member being stationary; wherein at least a portion of the pierceable seal is configured to transform from a non-activated state wherein the pierceable seal is intact, to an activated state wherein the pierceable seal is disrupted by the piercing member to create a sterile fluid communication between the sterile fluid conduit and the sterile fluid chamber of the sterile fluid container; and wherein the sterile fluid container comprises a plunger seal and translation of the plunger seal results in relative motion of the pierceable seal towards the piercing member. <EOS>