A cyclone separation system for separating live insects carried by an air stream, comprising: a main cyclone chamber having a top chamber part and a conical shaped bottom chamber part , wherein the top chamber part is connected to one or more intake channels (C) each of which is arranged for connection to a primary air source providing a primary air stream (A1) carrying live insects; a discharge nozzle having an intake end and a discharge end , the intake end being connected to the bottom chamber part , and wherein the discharge end comprises a discharge channel for discharging the live insects from the cyclone separation system , wherein the discharge end comprises an air injection member for connection to a secondary air source providing a secondary air stream (A2) to the air injection member , wherein the air injection member comprises an air chamber for connection to the secondary air source, and a plurality of primary air injection channels fluidly connecting the air chamber and the discharge channel , wherein each of the primary air injection channels is arranged to provide an injected upstream air flow (F) in an upstream direction (U) back into the discharge nozzle for stopping the discharge of the separated live insects, and wherein the discharge nozzle comprises an inner wall part extending between the intake end and the discharge channel , and wherein the inner wall part comprises a plurality of elongated secondary air injection channels fluidly connected to the air chamber , characterised in that each of said plurality of elongated secondary air injection channels is extending from the discharge channel in the upstream direction (U), and is arranged to provide an injected lateral air flow (V) along the inner wall part . a main cyclone chamber having a top chamber part and a conical shaped bottom chamber part , wherein the top chamber part is connected to one or more intake channels (C) each of which is arranged for connection to a primary air source providing a primary air stream (A1) carrying live insects; a discharge nozzle having an intake end and a discharge end , the intake end being connected to the bottom chamber part , and wherein the discharge end comprises a discharge channel for discharging the live insects from the cyclone separation system , wherein the discharge end comprises an air injection member for connection to a secondary air source providing a secondary air stream (A2) to the air injection member , wherein the air injection member comprises an air chamber for connection to the secondary air source, and a plurality of primary air injection channels fluidly connecting the air chamber and the discharge channel , wherein each of the primary air injection channels is arranged to provide an injected upstream air flow (F) in an upstream direction (U) back into the discharge nozzle for stopping the discharge of the separated live insects, and wherein the discharge nozzle comprises an inner wall part extending between the intake end and the discharge channel , and wherein the inner wall part comprises a plurality of elongated secondary air injection channels fluidly connected to the air chamber , characterised in that each of said plurality of elongated secondary air injection channels is extending from the discharge channel in the upstream direction (U), and is arranged to provide an injected lateral air flow (V) along the inner wall part . <EOS>