A substrate for sample analysis, which involves transportation of a liquid through rotational motion, the substrate for sample analysis comprising: a substrate including a rotation axis; a first chamber , which is positioned in the substrate and includes a first space configured to retain the liquid; a second chamber , which is positioned in the substrate and includes a second space configured to retain the liquid discharged from the first chamber ; and a first flow passage , which is positioned in the substrate , includes a path configured to connect the first chamber and the second chamber to each other, and is capable of being filled with the liquid retained in the first space through a capillary phenomenon, wherein the first flow passage has a first opening and a second opening , the first opening and the second opening are connected to the first chamber and the second chamber , respectively, and the first opening is positioned on a side closer to the rotation axis than the second opening , wherein the first space includes a first region , which is connected to the first opening and includes a portion extending from the first opening toward a side farther from the rotation axis, wherein the first space of the first chamber has a capacity larger than a capacity of the first flow passage , wherein the first flow passage includes a first portion having the first opening and a second portion having the second opening , wherein the second portion has a capillary force larger than a capillary force of the first portion , characterized in that the first flow passage further includes a space which is adjacent to the first portion , and which is positioned on the rotation axis side from the first portion and an opening communicating to the space , and wherein the space is free from being a capillary path. a substrate including a rotation axis; a first chamber , which is positioned in the substrate and includes a first space configured to retain the liquid; a second chamber , which is positioned in the substrate and includes a second space configured to retain the liquid discharged from the first chamber ; and a first flow passage , which is positioned in the substrate , includes a path configured to connect the first chamber and the second chamber to each other, and is capable of being filled with the liquid retained in the first space through a capillary phenomenon, wherein the first flow passage has a first opening and a second opening , the first opening and the second opening are connected to the first chamber and the second chamber , respectively, and the first opening is positioned on a side closer to the rotation axis than the second opening , wherein the first space includes a first region , which is connected to the first opening and includes a portion extending from the first opening toward a side farther from the rotation axis, wherein the first space of the first chamber has a capacity larger than a capacity of the first flow passage , wherein the first flow passage includes a first portion having the first opening and a second portion having the second opening , wherein the second portion has a capillary force larger than a capillary force of the first portion , characterized in that the first flow passage further includes a space which is adjacent to the first portion , and which is positioned on the rotation axis side from the first portion and an opening communicating to the space , and wherein the space is free from being a capillary path. <EOS>