A microfluidic device for concentrating and detecting particles contained in a fluid sample (FS), comprising a substrate configured for being set in rotation with respect to a centre of rotation , the substrate having a surface at which at least one microfluidic arrangement is defined, which extends substantially according to a plane identified by the substrate , wherein the at least one microfluidic arrangement comprises a plurality of microchannels each having a first end and a second end , wherein: - each microchannel of the plurality comprises, in a region thereof intermediate to its first end and its second end , at least one detection area that is at a first distance (R1) in a radial direction (R) with respect to the centre of rotation of the substrate ; - the first end and the second end of each microchannel of the plurality are at second distances (R2) in a radial direction (R) with respect to the centre of rotation of the substrate , said second distances (R2) being preferably the same as one another; - the first distance (R1) in a radial direction (R) is greater than the second distances (R2) in a radial direction (R), wherein: - the detection area is an area for particle accumulation, - the at least one microfluidic arrangement comprises at least one first duct or chamber and a second duct or chamber , the plurality of microchannels having the respective first ends and second ends connected in parallel to the first duct or chamber and to the second duct or chamber , respectively, one of the first duct or chamber and second duct or chamber being pre-arranged for receiving the fluid sample (FS), , - in the at least one detection area for particle accumulation, each microchannel of the plurality is substantially V-shaped or U-shaped, or comprises two substantially convergent microchannel branches , and - each microchannel of the plurality is open only at its first end and its second end , in such a way that particles (P) contained in a volume of fluid of the fluid sample (FS) that penetrates into at least one microchannel of the plurality concentrate in the at least one detection area as a result of the centrifugal force caused by a rotation of the substrate about the centre of rotation . - each microchannel of the plurality comprises, in a region thereof intermediate to its first end and its second end , at least one detection area that is at a first distance (R1) in a radial direction (R) with respect to the centre of rotation of the substrate ; - the first end and the second end of each microchannel of the plurality are at second distances (R2) in a radial direction (R) with respect to the centre of rotation of the substrate , said second distances (R2) being preferably the same as one another; - the first distance (R1) in a radial direction (R) is greater than the second distances (R2) in a radial direction (R), - the detection area is an area for particle accumulation, - the at least one microfluidic arrangement comprises at least one first duct or chamber and a second duct or chamber , the plurality of microchannels having the respective first ends and second ends connected in parallel to the first duct or chamber and to the second duct or chamber , respectively, one of the first duct or chamber and second duct or chamber being pre-arranged for receiving the fluid sample (FS), , - in the at least one detection area for particle accumulation, each microchannel of the plurality is substantially V-shaped or U-shaped, or comprises two substantially convergent microchannel branches , and - each microchannel of the plurality is open only at its first end and its second end , in such a way that particles (P) contained in a volume of fluid of the fluid sample (FS) that penetrates into at least one microchannel of the plurality concentrate in the at least one detection area as a result of the centrifugal force caused by a rotation of the substrate about the centre of rotation . <EOS>