A method of determining an analyte concentration from a fluid sample with a biosensor having at least two electrodes and a reagent disposed on at least one electrode of the electrodes, the method comprising: a) depositing a fluid sample on any one of the at least two electrodes to start an analyte test sequence; b) applying a first signal to the sample to derive a physical characteristic of the sample; c) obtaining a physical characteristic of the sample; d) specifying a sampling time based on the obtained physical characteristic; e) driving a second signal to the sample, wherein the second signal is applied from the start of the analyte test sequence; and f) measuring an output signal at the specified sampling time from at least one electrode of the at least two electrodes; g) calculating an analyte concentration based on the measured output signal, wherein i) the biosensor is a test strip and the specified sampling time is calculated using an equation of the form: SpecifiedSamplingTime = x 1 H x 2 + x 3 where "SpecifiedSamplingTime" is designated as a time point (in seconds) from the start of the test sequence at which to sample the output signal of the test strip, H represents the physical characteristic of the sample; x 1 is 4.3e5 (s) +/- 10%; x 2 is -3.9 +/- 10%; and x 3 is 4.8 (s) +/- 10%, or ii) the specified sampling time is looked up in look-up Table 1, wherein the specified sampling time is designated as a time point (in seconds) from the start of the test sequence at which to sample the output signal of the test strip: Table 1 Physical Characteristic Sampling Time T (seconds) 30 5.56 31 5.46 32 5.38 33 5.32 34 5.26 35 5.2 36 5.16 37 5.12 38 5.08 39 5.06 40 5.02 41 5 42 5 43 4.98 44 4.96 45 4.96 46 4.94 47 4.92 48 4.92 49 4.9 50 4.9 51 4.9 52 4.88 53 4.88 54 4.88 55 4.86 and wherein the physical characteristic comprises hematocrit and the analyte is glucose. a) depositing a fluid sample on any one of the at least two electrodes to start an analyte test sequence; b) applying a first signal to the sample to derive a physical characteristic of the sample; c) obtaining a physical characteristic of the sample; d) specifying a sampling time based on the obtained physical characteristic; e) driving a second signal to the sample, wherein the second signal is applied from the start of the analyte test sequence; and f) measuring an output signal at the specified sampling time from at least one electrode of the at least two electrodes; g) calculating an analyte concentration based on the measured output signal, wherein i) the biosensor is a test strip and the specified sampling time is calculated using an equation of the form: SpecifiedSamplingTime = x 1 H x 2 + x 3 where "SpecifiedSamplingTime" is designated as a time point (in seconds) from the start of the test sequence at which to sample the output signal of the test strip, H represents the physical characteristic of the sample; x 1 is 4.3e5 (s) +/- 10%; x 2 is -3.9 +/- 10%; and x 3 is 4.8 (s) +/- 10%, or ii) the specified sampling time is looked up in look-up Table 1, i) the biosensor is a test strip and the specified sampling time is calculated using an equation of the form: SpecifiedSamplingTime = x 1 H x 2 + x 3 where "SpecifiedSamplingTime" is designated as a time point (in seconds) from the start of the test sequence at which to sample the output signal of the test strip, H represents the physical characteristic of the sample; x 1 is 4.3e5 (s) +/- 10%; x 2 is -3.9 +/- 10%; and x 3 is 4.8 (s) +/- 10%, or "SpecifiedSamplingTime" is designated as a time point (in seconds) from the start of the test sequence at which to sample the output signal of the test strip, H represents the physical characteristic of the sample; x 1 is 4.3e5 (s) +/- 10%; x 2 is -3.9 +/- 10%; and x 3 is 4.8 (s) +/- 10%, or ii) the specified sampling time is looked up in look-up Table 1, <EOS>