A method for separating recombinant AAVrh10 (rAAVrh10) viral particles containing DNA comprising pharmacologically active genomic sequences from genome-deficient (empty) AAVrh10 capsid intermediates, said method comprising: (a) separating rAAVrh10 viral particles and AAVrh10 capsid intermediates from non-AAV contaminants obtained from an AAV producer cell culture which comprises an expression cassette encoding an AAVrh10 capsid protein operably linked to expression control sequences which direct expression thereof in the producer cell, an expression cassette comprising sequences for packaging in the AAVrh10 capsid, and an expression cassette comprising helper viruses necessary for production and packaging of the AAVrh10 capsid into a viral particle, wherein the separation is performed by a high performance affinity resin having an antibody for AAVrh10 to afford purified materials of (a); (b) forming a loading suspension comprising: a suspension which comprises (i) materials of step (a) purified by the high performance affinity resin and (ii) a first buffer comprising 20 mM Bis-Tris propane (BTP) and a pH in the range 9.8 to 10.2 or a pH of 10.0; (c) loading the mixture of step (b) onto a strong anion exchange resin, said resin being in a vessel having an inlet for flow of a suspension and/or solution and an outlet permitting flow of eluate from the vessel; (d) washing the loaded anion exchange resin with a buffer which comprises 10mM NaCl and 20mM BTP with a pH in the range 9.8 to 10.2 or a pH of 10.0; (e) applying an increasing salt concentration gradient to the loaded and washed anion exchange resin and monitoring the eluate for ionic strength; and (f) monitoring the eluate for peak ultraviolet absorbance at A260nm and a peak of A280nm and collecting the rAAV10 viral particles from a fraction which is eluted when the peak for the A260nm crosses over and exceeds the peak for A280nm, said rAAV viral particles being purified away from AAVrh10 capsid intermediates. (a) separating rAAVrh10 viral particles and AAVrh10 capsid intermediates from non-AAV contaminants obtained from an AAV producer cell culture which comprises an expression cassette encoding an AAVrh10 capsid protein operably linked to expression control sequences which direct expression thereof in the producer cell, an expression cassette comprising sequences for packaging in the AAVrh10 capsid, and an expression cassette comprising helper viruses necessary for production and packaging of the AAVrh10 capsid into a viral particle, wherein the separation is performed by a high performance affinity resin having an antibody for AAVrh10 to afford purified materials of (a); (b) forming a loading suspension comprising: a suspension which comprises (i) materials of step (a) purified by the high performance affinity resin and (ii) a first buffer comprising 20 mM Bis-Tris propane (BTP) and a pH in the range 9.8 to 10.2 or a pH of 10.0; (c) loading the mixture of step (b) onto a strong anion exchange resin, said resin being in a vessel having an inlet for flow of a suspension and/or solution and an outlet permitting flow of eluate from the vessel; (d) washing the loaded anion exchange resin with a buffer which comprises 10mM NaCl and 20mM BTP with a pH in the range 9.8 to 10.2 or a pH of 10.0; (e) applying an increasing salt concentration gradient to the loaded and washed anion exchange resin and monitoring the eluate for ionic strength; and (f) monitoring the eluate for peak ultraviolet absorbance at A260nm and a peak of A280nm and collecting the rAAV10 viral particles from a fraction which is eluted when the peak for the A260nm crosses over and exceeds the peak for A280nm, said rAAV viral particles being purified away from AAVrh10 capsid intermediates. <EOS>