A multi-spectral fundus imaging system using dynamic visual stimulation, comprising: a multi-spectral light source being a light source capable of emitting lights of multiple different wavelengths; a mid-pass mirror being a reflecting mirror with a central hole penetrating the reflecting mirror; an imaging focusing lens group ; an image acquisition device ; and a controller, configured to control the multi-spectral light source and the image acquisition device to work synchronously; wherein a monochromatic excitation light emitted by the multi-spectral light source is reflected into the fundus through the central hole of the mid-pass mirror ; a reflected imaging light passes through the central hole of the mid-pass mirror and the imaging focusing lens group in sequence; and the image acquisition device acquire an image and completes a multi-spectral fundus image acquisition; and the multi-spectral light source changes a wavelength of the monochromatic excitation light, and the image acquisition device simultaneously acquires corresponding multi-spectral fundus images of different wavelengths until all the wavelengths are acquired to form a multi-spectral static image; wherein the multi-spectral fundus imaging system further comprises an optical stimulation device ; and the controller controls the optical stimulation device and the image acquisition device to work synchronously, and a pattern sent by the optical stimulation device is transmitted to the fundus through the image focusing lens group and the central hole of the mid-pass mirror in sequence; the imaging light reflected from the fundus passes through the central hole of the mid-pass mirror and the imaging focusing lens group in sequence; the image acquisition device acquires the image to complete a multi-spectral fundus image acquisition. a multi-spectral light source being a light source capable of emitting lights of multiple different wavelengths; a mid-pass mirror being a reflecting mirror with a central hole penetrating the reflecting mirror; an imaging focusing lens group ; an image acquisition device ; and a controller, configured to control the multi-spectral light source and the image acquisition device to work synchronously; wherein a monochromatic excitation light emitted by the multi-spectral light source is reflected into the fundus through the central hole of the mid-pass mirror ; a reflected imaging light passes through the central hole of the mid-pass mirror and the imaging focusing lens group in sequence; and the image acquisition device acquire an image and completes a multi-spectral fundus image acquisition; and the multi-spectral light source changes a wavelength of the monochromatic excitation light, and the image acquisition device simultaneously acquires corresponding multi-spectral fundus images of different wavelengths until all the wavelengths are acquired to form a multi-spectral static image; wherein the multi-spectral fundus imaging system further comprises an optical stimulation device ; and the controller controls the optical stimulation device and the image acquisition device to work synchronously, and a pattern sent by the optical stimulation device is transmitted to the fundus through the image focusing lens group and the central hole of the mid-pass mirror in sequence; the imaging light reflected from the fundus passes through the central hole of the mid-pass mirror and the imaging focusing lens group in sequence; the image acquisition device acquires the image to complete a multi-spectral fundus image acquisition. <EOS>