A wireless communication system, comprising: a downlink base transceiver station interface configured to receive downlink electrical radio frequency signals from at least one base transceiver station; at least one optical interface module configured to: receive and convert the downlink electrical radio frequency signals from the downlink base transceiver station interface into downlink Radio-over-Fiber signals on at least one communication downlink; and receive and convert uplink Radio-over-Fiber signals from at least one remote antenna unit into uplink radio frequency electrical signals on at least one communication uplink; an uplink base transceiver station interface configured to receive and communicate the uplink electrical radio frequency signals from the at least one communication uplink to the at least one base transceiver station; and a controller configured to: cause at least one downlink calibration signal to be communicated over the at least one communication downlink and the at least one communication uplink; determine a total downlink loss for the at least one communication downlink; determine a downlink base transceiver station loss from the total downlink loss; calibrate a downlink base transceiver station calibration gain in the downlink base transceiver station interface based on the downlink base transceiver station loss, calibrate at least one remote antenna unit calibration gain in the at least one remote antenna unit as the total downlink loss minus the downlink base transceiver station loss; and calibrate the at least one uplink gain in the at least one communication uplink based on loss incurred in the at least one calibration signal in the at least one communication uplink. a downlink base transceiver station interface configured to receive downlink electrical radio frequency signals from at least one base transceiver station; at least one optical interface module configured to: receive and convert the downlink electrical radio frequency signals from the downlink base transceiver station interface into downlink Radio-over-Fiber signals on at least one communication downlink; and receive and convert uplink Radio-over-Fiber signals from at least one remote antenna unit into uplink radio frequency electrical signals on at least one communication uplink; receive and convert the downlink electrical radio frequency signals from the downlink base transceiver station interface into downlink Radio-over-Fiber signals on at least one communication downlink; and receive and convert uplink Radio-over-Fiber signals from at least one remote antenna unit into uplink radio frequency electrical signals on at least one communication uplink; an uplink base transceiver station interface configured to receive and communicate the uplink electrical radio frequency signals from the at least one communication uplink to the at least one base transceiver station; and a controller configured to: cause at least one downlink calibration signal to be communicated over the at least one communication downlink and the at least one communication uplink; determine a total downlink loss for the at least one communication downlink; determine a downlink base transceiver station loss from the total downlink loss; calibrate a downlink base transceiver station calibration gain in the downlink base transceiver station interface based on the downlink base transceiver station loss, calibrate at least one remote antenna unit calibration gain in the at least one remote antenna unit as the total downlink loss minus the downlink base transceiver station loss; and cause at least one downlink calibration signal to be communicated over the at least one communication downlink and the at least one communication uplink; determine a total downlink loss for the at least one communication downlink; determine a downlink base transceiver station loss from the total downlink loss; calibrate a downlink base transceiver station calibration gain in the downlink base transceiver station interface based on the downlink base transceiver station loss, calibrate at least one remote antenna unit calibration gain in the at least one remote antenna unit as the total downlink loss minus the downlink base transceiver station loss; and