A method for correction of relative object-detector motion between successive views in an optical projection tomographic microscopy system that includes a microcapillary tube having a tube axis, wherein the successive views comprise a current view and a previous view, wherein the current view and the previous view are each taken from a different perspective, the method comprising the steps of: illuminating an object of interest contained within the microcapillary tube to produce acquired images including a current image from the current view and a previous image from the previous view; determining a lateral offset correction value for each of the current and previous images, where the lateral offset is a translation along the image dimension perpendicular to the tube axis, by thresholding each image and cross-correlating each thresholded image with a template image (22,23,24,25,26,27) along the rows of each of the thresholded image and the template image, wherein the template image is created by creating two bright lines separated by a known amount found between the walls of the microcapillary tube in one of the acquired images to form a preliminary template image, expanding the preliminary template image to provide zero-padding in two dimensions resulting in an expanded template image, and performing a two-dimensional FFT on the expanded template image to create the final template image; determining an axial offset correction value for the current image where the axial offset is a translation along the image dimension parallel to the tube axis, by thresholding a copy of the current image to produce a thresholded version of the current image, cross-correlating the thresholded version of the current image with a thresholded version of the previous image and determining an axial offset as a maximum in the cross-correlation function along the line that corresponds to the difference in lateral offset correction values of the current and previous images; and applying the lateral offset correction value and the axial offset correction value for the current image to the current image to produce a corrected file image. illuminating an object of interest contained within the microcapillary tube to produce acquired images including a current image from the current view and a previous image from the previous view; determining a lateral offset correction value for each of the current and previous images, where the lateral offset is a translation along the image dimension perpendicular to the tube axis, by thresholding each image and cross-correlating each thresholded image with a template image (22,23,24,25,26,27) along the rows of each of the thresholded image and the template image, wherein the template image is created by creating two bright lines separated by a known amount found between the walls of the microcapillary tube in one of the acquired images to form a preliminary template image, expanding the preliminary template image to provide zero-padding in two dimensions resulting in an expanded template image, and performing a two-dimensional FFT on the expanded template image to create the final template image; determining an axial offset correction value for the current image where the axial offset is a translation along the image dimension parallel to the tube axis, by thresholding a copy of the current image to produce a thresholded version of the current image, cross-correlating the thresholded version of the current image with a thresholded version of the previous image and determining an axial offset as a maximum in the cross-correlation function along the line that corresponds to the difference in lateral offset correction values of the current and previous images; and applying the lateral offset correction value and the axial offset correction value for the current image to the current image to produce a corrected file image.