A control method for a microfluidic detection system, the microfluidic detection system comprising a microfluidic biochip, a sample liquid driving apparatus and a detection mechanism, the microfluidic biochip being provided with a sample inlet for receiving a sample liquid, a communication port communicated with the sample liquid driving apparatus and a detection pool formed inside the microfluidic biochip, and the sample inlet, the detection pool and the communication port being communicated in sequence; wherein the microfluidic biochip is further provided with a reaction pool connected between the detection pool and the sample inlet, and the sample inlet, the reaction pool, the detection pool and the communication port are sequentially communicated through a micro-channel; the control method comprising: starting the sample liquid driving apparatus in a state where the sample inlet is in contact with the sample liquid, so as to drive the sample liquid to flow into the reaction pool by the sample liquid driving apparatus; switching the microfluidic biochip to a state where the sample inlet thereof is separated from the sample liquid; controlling the sample liquid driving apparatus to execute a third liquid drawing action for promoting the sample liquid to flow towards the reaction pool, such that at least part of the sample liquid in the micro-channel connected between the reaction pool and the sample inlet flows into the reaction pool, thus forming a preset space margin in the micro-channel, the preset space margin being used for accommodating the sample liquid pushed out by the sample liquid driving apparatus when the sample liquid driving apparatus executes a second liquid pushing action; controlling the sample liquid driving apparatus to periodically and repeatedly execute the second liquid pushing and drawing operation until the number of the second liquid pushing and drawing operations reaches a second preset number; the second liquid pushing and drawing operation comprising a second liquid pushing action for promoting the sample liquid to flow towards the sample inlet and a second liquid drawing action for promoting the sample liquid to flow towards the reaction pool; driving the sample liquid with the sample liquid driving apparatus to flow into the detection pool; detecting the detection pool with the detection mechanism; and controlling the sample liquid driving apparatus to periodically and repeatedly execute a first liquid pushing and drawing operation, the first liquid pushing and drawing operation comprising a first liquid pushing action used for prompting the sample liquid to flow in the direction of the sample inlet, and a first liquid drawing action used for prompting the sample liquid to flow in the direction of the detection pool. starting the sample liquid driving apparatus in a state where the sample inlet is in contact with the sample liquid, so as to drive the sample liquid to flow into the reaction pool by the sample liquid driving apparatus; switching the microfluidic biochip to a state where the sample inlet thereof is separated from the sample liquid; controlling the sample liquid driving apparatus to execute a third liquid drawing action for promoting the sample liquid to flow towards the reaction pool, such that at least part of the sample liquid in the micro-channel connected between the reaction pool and the sample inlet flows into the reaction pool, thus forming a preset space margin in the micro-channel, the preset space margin being used for accommodating the sample liquid pushed out by the sample liquid driving apparatus when the sample liquid driving apparatus executes a second liquid pushing action; controlling the sample liquid driving apparatus to periodically and repeatedly execute the second liquid pushing and drawing operation until the number of the second liquid pushing and drawing operations reaches a second preset number; the second liquid pushing and drawing operation comprising a second liquid pushing action for promoting the sample liquid to flow towards the sample inlet and a second liquid drawing action for promoting the sample liquid to flow towards the reaction pool; driving the sample liquid with the sample liquid driving apparatus to flow into the detection pool; detecting the detection pool with the detection mechanism; and controlling the sample liquid driving apparatus to periodically and repeatedly execute a first liquid pushing and drawing operation, the first liquid pushing and drawing operation comprising a first liquid pushing action used for prompting the sample liquid to flow in the direction of the sample inlet, and a first liquid drawing action used for prompting the sample liquid to flow in the direction of the detection pool.