A method for assessment of a number or density of immune cells in tumoral tissues comprising the steps consisting in: a. providing one or more immunostained slices of tissue section obtained by an automated slide-staining system by using antibodies binding specifically to markers expressed by immune cells, b. proceeding to digitalisation of the slides of step a. by high resolution scan capture, whereby a high definition digital picture of the slide to be analysed is obtained, c. detecting the slice of tissue section on the digital picture d. analyzing the slice of tissue section for defining (i) a tumour and (ii) an invasive margin of the tumour, e. providing a size reference grid with uniformly distributed units having a same surface, said grid being adapted to the size of the tumour to be analyzed, e1. checking the quality of immunostaining, by: - calculating the distribution of the staining intensity of positive cells detected for each unit of the grid and for the total tumour region, representing said distribution, - for each slide, calculating the mean, median, minimum and maximum values of the staining intensity of all positive stained cells detected, - comparing the values and the distribution of the staining intensity to a reference of normal range intensity determined for each marker, and f. detecting and quantifying stained cells of each unit whereby the number or the density of immune cells stained of each unit is assessed. a. providing one or more immunostained slices of tissue section obtained by an automated slide-staining system by using antibodies binding specifically to markers expressed by immune cells, b. proceeding to digitalisation of the slides of step a. by high resolution scan capture, whereby a high definition digital picture of the slide to be analysed is obtained, c. detecting the slice of tissue section on the digital picture d. analyzing the slice of tissue section for defining (i) a tumour and (ii) an invasive margin of the tumour, e. providing a size reference grid with uniformly distributed units having a same surface, said grid being adapted to the size of the tumour to be analyzed, e1. checking the quality of immunostaining, by: - calculating the distribution of the staining intensity of positive cells detected for each unit of the grid and for the total tumour region, representing said distribution, - for each slide, calculating the mean, median, minimum and maximum values of the staining intensity of all positive stained cells detected, - comparing the values and the distribution of the staining intensity to a reference of normal range intensity determined for each marker, and - calculating the distribution of the staining intensity of positive cells detected for each unit of the grid and for the total tumour region, representing said distribution, - for each slide, calculating the mean, median, minimum and maximum values of the staining intensity of all positive stained cells detected, - comparing the values and the distribution of the staining intensity to a reference of normal range intensity determined for each marker, and f. detecting and quantifying stained cells of each unit whereby the number or the density of immune cells stained of each unit is assessed.