A fitting assembly (200, 400, 500, 600, 700, 800) for use in a fluid transfer assembly having a fluid conduit, the fitting assembly comprising: (a) a first fitting (204, 404, 504, 604, 704, 804) having first and second ends and a bore extending between the first and second ends for receiving the fluid conduit, the first end configured to sealingly dispose a ferrule assembly (40, 640, 840) within a second fitting in an engaged position; wherein the first end of the first fitting is configured to bias the ferrule assembly into releasable locking engagement with the fluid conduit in the engaged position, wherein the ferrule assembly comprises: (i) a frusto-conical seal portion (48, 648, 848) having a tapered end, an enlarged end, and a first bore sized and configured to receive the fluid conduit, the seal portion formed from a first material; and (ii) a frusto-conical collet portion (52, 652, 852) having a tapered end and an enlarged end, the enlarged end of the collet portion mated with the enlarged end of the seal portion, the collet portion having a second bore in substantial alignment with the first bore, the collet portion including at least one axial finger extending along the tapered end that is configured to be biased into locking engagement with the fluid conduit, wherein the collet portion is formed from a second material; (b) a third fitting (212, 412, 512, 612, 712, 812) having first and second ends and a bore extending between the first and second ends for receiving the fluid conduit, the first end configured to sealingly dispose a collet assembly (216, 40, 518, 816) within the first fitting in an engaged position. (a) a first fitting (204, 404, 504, 604, 704, 804) having first and second ends and a bore extending between the first and second ends for receiving the fluid conduit, the first end configured to sealingly dispose a ferrule assembly (40, 640, 840) within a second fitting in an engaged position; wherein the first end of the first fitting is configured to bias the ferrule assembly into releasable locking engagement with the fluid conduit in the engaged position, wherein the ferrule assembly comprises: (i) a frusto-conical seal portion (48, 648, 848) having a tapered end, an enlarged end, and a first bore sized and configured to receive the fluid conduit, the seal portion formed from a first material; and (ii) a frusto-conical collet portion (52, 652, 852) having a tapered end and an enlarged end, the enlarged end of the collet portion mated with the enlarged end of the seal portion, the collet portion having a second bore in substantial alignment with the first bore, the collet portion including at least one axial finger extending along the tapered end that is configured to be biased into locking engagement with the fluid conduit, wherein the collet portion is formed from a second material; (i) a frusto-conical seal portion (48, 648, 848) having a tapered end, an enlarged end, and a first bore sized and configured to receive the fluid conduit, the seal portion formed from a first material; and (ii) a frusto-conical collet portion (52, 652, 852) having a tapered end and an enlarged end, the enlarged end of the collet portion mated with the enlarged end of the seal portion, the collet portion having a second bore in substantial alignment with the first bore, the collet portion including at least one axial finger extending along the tapered end that is configured to be biased into locking engagement with the fluid conduit, wherein the collet portion is formed from a second material; (b) a third fitting (212, 412, 512, 612, 712, 812) having first and second ends and a bore extending between the first and second ends for receiving the fluid conduit, the first end configured to sealingly dispose a collet assembly (216, 40, 518, 816) within the first fitting in an engaged position.