An aseptic coupling arrangement in which a first aseptic coupling device is to be coupled to a second aseptic coupling device for establishing a sterile connection, wherein: the first aseptic coupling device (100, 1210) comprises: a first main body defining a first face and a first fluid passage therethrough; a first sealing member received at least partially within the first main body; a first membrane coupled to the first face and covering the first sealing member; and a locking ring connected to a ring adapter and configured to couple the first aseptic coupling device to the second aseptic coupling device, an indexing feature (150, 152) that indexes a rotational position of the locking ring to an indexed position on the first main body; and the second aseptic coupling device (200, 1220) comprises: a second main body defining a second face and a second fluid passage therethrough; a second sealing member received at least partially within the second main body; a second membrane coupled to the second face to cover the second sealing member, wherein the first and second aseptic coupling devices are configured to be coupled together by rotating the locking ring from the initial indexed position to a secured position; characterized by the locking ring being rotatable about the first main body from i) the initial indexed position in which a portion of the first aseptic device can be inserted in the second aseptic coupling device to ii) the secured position in which a compressive force is created that is sufficient to form a seal of the first sealing member with a mating second sealing member but to still allow the first and second membrane to be removed. the first aseptic coupling device (100, 1210) comprises: a first main body defining a first face and a first fluid passage therethrough; a first sealing member received at least partially within the first main body; a first membrane coupled to the first face and covering the first sealing member; and a locking ring connected to a ring adapter and configured to couple the first aseptic coupling device to the second aseptic coupling device, an indexing feature (150, 152) that indexes a rotational position of the locking ring to an indexed position on the first main body; and a first main body defining a first face and a first fluid passage therethrough; a first sealing member received at least partially within the first main body; a first membrane coupled to the first face and covering the first sealing member; and a locking ring connected to a ring adapter and configured to couple the first aseptic coupling device to the second aseptic coupling device, an indexing feature (150, 152) that indexes a rotational position of the locking ring to an indexed position on the first main body; and the second aseptic coupling device (200, 1220) comprises: a second main body defining a second face and a second fluid passage therethrough; a second sealing member received at least partially within the second main body; a second membrane coupled to the second face to cover the second sealing member, a second main body defining a second face and a second fluid passage therethrough; a second sealing member received at least partially within the second main body; a second membrane coupled to the second face to cover the second sealing member, wherein the first and second aseptic coupling devices are configured to be coupled together by rotating the locking ring from the initial indexed position to a secured position; characterized by the locking ring being rotatable about the first main body from i) the initial indexed position in which a portion of the first aseptic device can be inserted in the second aseptic coupling device to ii) the secured position in which a compressive force is created that is sufficient to form a seal of the first sealing member with a mating second sealing member but to still allow the first and second membrane to be removed.