A method for three-dimensional imaging of a transparent biological object in a biological sample by full-field optical tomography, the three-dimensional imaging method comprising: - positioning the sample in the vicinity of an object focal plane of a microscope lens, said microscope lens comprising a given optical axis (Δ); - illuminating the sample in transmission by means of an illumination beam of spatially incoherent light with a given central wavelength (λ); - relatively displacing said microscope lens relative to said sample, along an axial direction parallel to the optical axis of the microscope lens, to define a plurality of positions of the sample, each position corresponding to a section of said biological object centred on the object focal plane of the microscope lens; and - for each position of the sample, producing at least one first image of an object field of said section comprising: - acquiring, by means of a two-dimensional acquisition device comprising a plurality of elementary detectors arranged in a detection plane, a plurality of two-dimensional interferometric signals resulting from optical interference between the illumination beam incident on the object field and a beam scattered by said object field, wherein said detection plane is optically conjugated with the object focal plane of the microscope lens by an imaging optical system comprising said microscope lens; - calculating, by means of a processing unit, said at least one first image, from said plurality of two-dimensional interferometric signals. - positioning the sample in the vicinity of an object focal plane of a microscope lens, said microscope lens comprising a given optical axis (Δ); - illuminating the sample in transmission by means of an illumination beam of spatially incoherent light with a given central wavelength (λ); - relatively displacing said microscope lens relative to said sample, along an axial direction parallel to the optical axis of the microscope lens, to define a plurality of positions of the sample, each position corresponding to a section of said biological object centred on the object focal plane of the microscope lens; and - for each position of the sample, producing at least one first image of an object field of said section comprising: - acquiring, by means of a two-dimensional acquisition device comprising a plurality of elementary detectors arranged in a detection plane, a plurality of two-dimensional interferometric signals resulting from optical interference between the illumination beam incident on the object field and a beam scattered by said object field, wherein said detection plane is optically conjugated with the object focal plane of the microscope lens by an imaging optical system comprising said microscope lens; - calculating, by means of a processing unit, said at least one first image, from said plurality of two-dimensional interferometric signals. - acquiring, by means of a two-dimensional acquisition device comprising a plurality of elementary detectors arranged in a detection plane, a plurality of two-dimensional interferometric signals resulting from optical interference between the illumination beam incident on the object field and a beam scattered by said object field, wherein said detection plane is optically conjugated with the object focal plane of the microscope lens by an imaging optical system comprising said microscope lens; - calculating, by means of a processing unit, said at least one first image, from said plurality of two-dimensional interferometric signals.