A two-shot tube retention fastener molding method, wherein the tube retention fastener includes a first shot body member having a pair of deflecting wings, with each of the pair of deflecting wings extending angularly toward each other from one of a pair of side walls, and a second shot isolation member bonded along an interior surface of each of the pair of deflecting wings, the method comprising: molding the first shot body member, including the pair of deflecting wings; molding the second shot isolation member while: supporting an exterior wing surface of each of the pair of deflecting wings against a first mold surface; supporting a first interior wing surface portion of each of the pair of deflecting wings against a second mold surface and leaving a second interior wing surface portion of each of the pair of deflecting wings unsupported to define a portion of a mold cavity of the second shot member extending from a distal end of the deflecting wing to the pair of side walls, wherein the isolation member is bonded to a continuous portion of the interior surface of each deflecting wing to its corner or junction with its side wall, wherein the continuous portion of the interior surface of the body member to which the isolation member is bonded further extends from each corner over each of the side walls and over the semi-circular sleeve, wherein each corner defines a hinge point for both the body portion and the isolation member portion bonded at the corner to the body member of the deflecting wing, wherein each of the pair of deflecting wings has a deflected position in which a wing portion of the isolation member that is bonded to each deflecting wing along a first span between a corner and a distal end of the deflecting wing is able to fold flat in a face-to-face relationship against a side wall portion of the isolation member that is bonded to an adjacent side wall along a second span between the corner and the semi-circular sleeve. molding the first shot body member, including the pair of deflecting wings; molding the second shot isolation member while: supporting an exterior wing surface of each of the pair of deflecting wings against a first mold surface; supporting a first interior wing surface portion of each of the pair of deflecting wings against a second mold surface and leaving a second interior wing surface portion of each of the pair of deflecting wings unsupported to define a portion of a mold cavity of the second shot member extending from a distal end of the deflecting wing to the pair of side walls, supporting an exterior wing surface of each of the pair of deflecting wings against a first mold surface; supporting a first interior wing surface portion of each of the pair of deflecting wings against a second mold surface and leaving a second interior wing surface portion of each of the pair of deflecting wings unsupported to define a portion of a mold cavity of the second shot member extending from a distal end of the deflecting wing to the pair of side walls, wherein the isolation member is bonded to a continuous portion of the interior surface of each deflecting wing to its corner or junction with its side wall, wherein the continuous portion of the interior surface of the body member to which the isolation member is bonded further extends from each corner over each of the side walls and over the semi-circular sleeve, wherein each corner defines a hinge point for both the body portion and the isolation member portion bonded at the corner to the body member of the deflecting wing, wherein each of the pair of deflecting wings has a deflected position in which a wing portion of the isolation member that is bonded to each deflecting wing along a first span between a corner and a distal end of the deflecting wing is able to fold flat in a face-to-face relationship against a side wall portion of the isolation member that is bonded to an adjacent side wall along a second span between the corner and the semi-circular sleeve.