A method of isolating a target cell, wherein the target cell has a receptor molecule on the target cell surface, the method comprising: - incubating i) a receptor molecule binding reagent, the receptor molecule binding reagent comprising a binding site B and a binding partner C, wherein the binding site B comprised in the receptor molecule binding reagent is capable of specifically binding to the receptor molecule on the target cell surface, and wherein the binding partner C comprised in the receptor molecule binding reagent is capable of reversibly binding to a binding site Z on a multimerization reagent, ii) a multimerization reagent, wherein the multimerization reagent comprises two or more binding sites Z capable of reversibly binding to the binding partner C comprised in the receptor molecule binding reagent, wherein the multimerization reagent further comprises a ligand binding partner LB, the ligand binding partner LB being capable of specifically binding a ligand L, and iii) a sample, the sample comprising the target cell, thereby allowing the receptor molecule binding reagent, the multimerization reagent and the target cell to form a multivalent binding complex comprising the target cell bound to two or more receptor molecule binding reagents that are bound to the multimerization reagent, - contacting the multivalent binding complex of target cell, receptor molecule binding reagent and multimerization reagent with a solid phase, the solid phase comprising the ligand L, wherein the solid phase is suitable for chromatography, and wherein the solid phase is a non-magnetic material or non-magnetisable material, thereby allowing reversible immobilization of the target cell on the solid phase via the binding between the ligand L and the ligand binding partner LB, wherein 1 immobilization of the target cell on the solid phase is reversible upon disruption of at least the binding between the binding partner C comprised in the receptor molecule binding reagent and the binding site Z of the multimerization reagent. - incubating i) a receptor molecule binding reagent, the receptor molecule binding reagent comprising a binding site B and a binding partner C, wherein the binding site B comprised in the receptor molecule binding reagent is capable of specifically binding to the receptor molecule on the target cell surface, and wherein the binding partner C comprised in the receptor molecule binding reagent is capable of reversibly binding to a binding site Z on a multimerization reagent, ii) a multimerization reagent, wherein the multimerization reagent comprises two or more binding sites Z capable of reversibly binding to the binding partner C comprised in the receptor molecule binding reagent, wherein the multimerization reagent further comprises a ligand binding partner LB, the ligand binding partner LB being capable of specifically binding a ligand L, and iii) a sample, the sample comprising the target cell, thereby allowing the receptor molecule binding reagent, the multimerization reagent and the target cell to form a multivalent binding complex comprising the target cell bound to two or more receptor molecule binding reagents that are bound to the multimerization reagent, i) a receptor molecule binding reagent, the receptor molecule binding reagent comprising a binding site B and a binding partner C, wherein the binding site B comprised in the receptor molecule binding reagent is capable of specifically binding to the receptor molecule on the target cell surface, and wherein the binding partner C comprised in the receptor molecule binding reagent is capable of reversibly binding to a binding site Z on a multimerization reagent, ii) a multimerization reagent, wherein the multimerization reagent comprises two or more binding sites Z capable of reversibly binding to the binding partner C comprised in the receptor molecule binding reagent, wherein the multimerization reagent further comprises a ligand binding partner LB, the ligand binding partner LB being capable of specifically binding a ligand L, and iii) a sample, the sample comprising the target cell, thereby allowing the receptor molecule binding reagent, the multimerization reagent and the target cell to form a multivalent binding complex comprising the target cell bound to two or more receptor molecule binding reagents that are bound to the multimerization reagent, - contacting the multivalent binding complex of target cell, receptor molecule binding reagent and multimerization reagent with a solid phase, the solid phase comprising the ligand L, wherein the solid phase is suitable for chromatography, and wherein the solid phase is a non-magnetic material or non-magnetisable material,