A computer-implemented method, comprising: - analyzing a first image taken of a first set of particles having a uniform concentration of fluorescence material associated therewith to identify one or more pixels within the first image that exhibit an optical parameter value above a first predetermined threshold; - separating the first image into a first array of subsections of the first image, wherein the subsections include respective collections of pixels identified during the step of analyzing the first image; - developing for each respective subsection within the first image a statistic representative of the level of the optical parameter for the collections of pixels categorized thereto; characterized in that the method further comprises - analyzing a second image taken of a second set of particles having a uniform concentration of fluorescence material associated therewith to identify one or more pixels within the second image that exhibit an optical parameter value above the first predetermined threshold, wherein the second image is formed using an illumination source of a different wavelength than the first image; - separating the second image into a second array of subsections, wherein the subsections of the second array include respective collections of pixels identified during the step of analyzing the second image, and wherein dimensions of the subsections in the second array are substantially equal to the dimensions of the subsections of the first array; - developing for each respective subsection within the second image a statistic representative of the level of the optical parameter for the collections of pixels categorized thereto; - analyzing a third image taken for a third set of particles having an unknown concentration of fluorescence-material associated therewith to identify one or more pixels within the third image that exhibit an optical parameter value above the first predetermined threshold; - separating the third image into a third array of subsections of the third image, wherein the subsections include respective collections of pixels identified during the step of analyzing the third image and, wherein dimensions of the subsections in the third image are substantially equal to the dimensions of the subsections of the first image; and - dividing the optical parameter value for each of the pixels identified during the step of analyzing the third image by the statistic developed for the corresponding image subsection of the first and second sets of images to obtain a normalized value for the optical parameter. - analyzing a first image taken of a first set of particles having a uniform concentration of fluorescence material associated therewith to identify one or more pixels within the first image that exhibit an optical parameter value above a first predetermined threshold; - separating the first image into a first array of subsections of the first image, wherein the subsections include respective collections of pixels identified during the step of analyzing the first image; - developing for each respective subsection within the first image a statistic representative of the level of the optical parameter for the collections of pixels categorized thereto; - analyzing a second image taken of a second set of particles having a uniform concentration of fluorescence material associated therewith to identify one or more pixels within the second image that exhibit an optical parameter value above the first predetermined threshold, wherein the second image is formed using an illumination source of a different wavelength than the first image; - separating the second image into a second array of subsections, wherein the subsections of the second array include respective collections of pixels identified during the step of analyzing the second image, and wherein dimensions of the subsections in the second array are substantially equal to the dimensions of the subsections of the first array; - developing for each respective subsection within the second image a statistic representative of the level of the optical parameter for the collections of pixels categorized thereto; - analyzing a third image taken for a third set of particles having an unknown concentration of fluorescence-material associated therewith to identify one or more pixels within the third image that exhibit an optical parameter value above the first predetermined threshold; - separating the third image into a third array of subsections of the third image, wherein the subsections include respective collections of pixels identified during the step of analyzing the third image and, wherein dimensions of the subsections in the third image are substantially equal to the dimensions of the subsections of the first image; and - dividing the optical parameter value for each of the pixels identified during the step of analyzing the third image by the statistic developed for the corresponding image subsection of the first and second sets of images to obtain a normalized value for the optical parameter.