A probe set for analyzing a genetic locus of a target polynucleotide sequence, comprising: one or more first probes comprising: a first hybridization sequence that specifically binds to the target polynucleotide sequence upstream of or including the genetic locus, and a first primer sequence upstream of the first hybridization sequence, wherein the first primer sequence does not bind to the target polynucleotide sequence; and one or more second probes comprising: (i) a second hybridization sequence that specifically binds to the target polynucleotide sequence downstream of or starting from the genetic locus, and (ii) a second primer sequence downstream of the second hybridization sequence, wherein the second primer sequence does not bind to the target polynucleotide sequence, wherein the genetic locus comprises 1494C>T in the 12SrRNA gene, IVS7-2A> G in the SLC26A4 gene, 235delC in the GJB2 gene, 176DEL16 in the GJB2 gene, or 299delAT in the GJB2 gene; and the one or more first probes for 1494C>T in the 12SrRNA gene comprises the polynucleotide sequence set forth in SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, and/or SEQ ID NO: 24, and the one or more second probes for 1494C>T in the 12SrRNA gene comprises the polynucleotide sequence set forth in SEQ ID NO: 25; the one or more first probes for IVS7-2A> G in the SLC26A4 gene comprises the polynucleotide sequence set forth in SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, and/or SEQ ID NO: 10, and the one or more second probes for IVS7-2A> G in the SLC26A4 gene comprises the polynucleotide sequence set forth in SEQ ID NO: 11; the one or more first probes for 235delC in the GJB2 gene comprises the polynucleotide sequence set forth in SEQ ID NO: 15 and/or SEQ ID NO:16, and the one or more second probes for 235delC in the GJB2 gene comprises the polynucleotide sequence set forth in SEQ ID NO: 17; the one or more first probes for 176DEL16 in the GJB2 gene comprises the polynucleotide sequence set forth in SEQ ID NO: 12 and/or SEQ ID NO:13, and the one or more second probes for 176DEL16 in the GJB2 gene comprises the polynucleotide sequence set forth in SEQ ID NO: 14; the one or more first probes for 299delAT in the GJB2 gene comprises the polynucleotide sequence set forth in SEQ ID NO: 18 and/or SEQ ID NO:19, and the one or more second probes for 299delAT in the GJB2 gene comprises the polynucleotide sequence set forth in SEQ ID NO: 20; the extension directions of the first and second probes are the same; the first probe is upstream of the second probe; and the first and second probes are adjacent and, when coupled, form a sequence comprising the genetic locus. one or more first probes comprising: a first hybridization sequence that specifically binds to the target polynucleotide sequence upstream of or including the genetic locus, and a first primer sequence upstream of the first hybridization sequence, wherein the first primer sequence does not bind to the target polynucleotide sequence; and one or more second probes comprising: (i) a second hybridization sequence that specifically binds to the target polynucleotide sequence downstream of or starting from the genetic locus, and (ii) a second primer sequence downstream of the second hybridization sequence, wherein the second primer sequence does not bind to the target polynucleotide sequence, wherein the genetic locus comprises 1494C>T in the 12SrRNA gene, IVS7-2A> G in the SLC26A4 gene, 235delC in the GJB2 gene, 176DEL16 in the GJB2 gene, or 299delAT in the GJB2 gene; and the one or more first probes for 1494C>T in the 12SrRNA gene comprises the polynucleotide sequence set forth in SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, and/or SEQ ID NO: 24, and the one or more second probes for 1494C>T in the 12SrRNA gene comprises the polynucleotide sequence set forth in SEQ ID NO: 25; the one or more first probes for IVS7-2A> G in the SLC26A4 gene comprises the polynucleotide sequence set forth in SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, and/or SEQ ID NO: 10, and the one or more second probes for IVS7-2A> G in the SLC26A4 gene comprises the polynucleotide sequence set forth in SEQ ID NO: 11; the one or more first probes for 235delC in the GJB2 gene comprises the polynucleotide sequence set forth in SEQ ID NO: 15 and/or SEQ ID NO:16, and the one or more second probes for 235delC in the GJB2 gene comprises the polynucleotide sequence set forth in SEQ ID NO: 17; the one or more first probes for 176DEL16 in the GJB2 gene comprises the polynucleotide sequence set forth in SEQ ID NO: 12 and/or SEQ ID NO:13, and the one or more second probes for 176DEL16 in the GJB2 gene comprises the polynucleotide sequence set forth in SEQ ID NO: 14; the one or more first probes for 299delAT in the GJB2 gene comprises the polynucleotide sequence set forth in SEQ ID NO: 18 and/or SEQ ID NO:19, and the one or more second probes for 299delAT in the GJB2 gene comprises the polynucleotide sequence set forth in SEQ ID NO: 20; the extension directions of the first and second probes are the same; the first probe is upstream of the second probe; and the first and second probes are adjacent and, when coupled, form a sequence comprising the genetic locus.