An integrated biocontainment cell sorter flow cytometer comprising: a main cabinet of said integrated biocontainment cell sorter that is not hermetically sealed; an input sample area disposed in said main cabinet; a moveable partition in said main cabinet that moves in an access opening of said main cabinet, said moveable partition covering a constant amount of area of said access opening as said moveable partition is moved in said access opening which leaves a constant amount of area of said access opening that is not covered by said moveable partition and is open, as said moveable partition is moved in said access opening; a first fan configured to draw air from said main cabinet to create a first low pressure in said main cabinet that is substantially constant as said moveable partition is moved in said access opening, which limits contaminated air in said main cabinet from escaping from said main cabinet; an aerosol management containment area that is not hermetically sealed, which is disposed in said main cabinet, said aerosol management containment area having openings (135, 137, 160) that are connected to said main cabinet so that said aerosol management containment area is disposed in and subject to said first low pressure, said aerosol management containment area having a nozzle configured to create a droplet stream containing sample cells, sort plates configured to separate said droplet stream into deflected streams and collection media configured to collect said deflected streams; a second fan configured to draw air from said aerosol management containment area to create a second low pressure in said aerosol management containment area that is lower than said first low pressure which causes air from said main cabinet to flow from said main cabinet to said aerosol management containment area and limits contaminated air from flowing from said aerosol management containment area into said main cabinet; optical excitation devices located outside of said main cabinet and said aerosol management containment area to allow access to said optical excitement devices without accessing said main cabinet or said aerosol management containment area. a main cabinet of said integrated biocontainment cell sorter that is not hermetically sealed; an input sample area disposed in said main cabinet; a moveable partition in said main cabinet that moves in an access opening of said main cabinet, said moveable partition covering a constant amount of area of said access opening as said moveable partition is moved in said access opening which leaves a constant amount of area of said access opening that is not covered by said moveable partition and is open, as said moveable partition is moved in said access opening; a first fan configured to draw air from said main cabinet to create a first low pressure in said main cabinet that is substantially constant as said moveable partition is moved in said access opening, which limits contaminated air in said main cabinet from escaping from said main cabinet; an aerosol management containment area that is not hermetically sealed, which is disposed in said main cabinet, said aerosol management containment area having openings (135, 137, 160) that are connected to said main cabinet so that said aerosol management containment area is disposed in and subject to said first low pressure, said aerosol management containment area having a nozzle configured to create a droplet stream containing sample cells, sort plates configured to separate said droplet stream into deflected streams and collection media configured to collect said deflected streams; a second fan configured to draw air from said aerosol management containment area to create a second low pressure in said aerosol management containment area that is lower than said first low pressure which causes air from said main cabinet to flow from said main cabinet to said aerosol management containment area and limits contaminated air from flowing from said aerosol management containment area into said main cabinet; optical excitation devices located outside of said main cabinet and said aerosol management containment area to allow access to said optical excitement devices without accessing said main cabinet or said aerosol management containment area.