A method for detecting an underfill condition in a biosensor, comprising: providing a biosensor system in form of an analytical instrument including a sensor strip having a sample interface on a base, the base forming a reservoir and a channel with an opening, the base further having a surface on which at least one working electrode and at least one counter electrode are disposed, the sample interface having conductors connected to the working electrode and the counter electrode, a biological fluid sample received in the opening flowing through the channel to fill at least in part the reservoir, and a measuring device in electrical or optical communication with the reservoir, the measurement device having electrical circuitry including a processor and a storage medium, the electrical circuitry being communicatively coupled to a signal generator and a sensor interface, the processor having instructions and data stored in the storage medium; detecting, by the biosensor, when a sample of a biological fluid is available for analysis, applying, via the processor, a pulse sequence to the biosensor system, the pulse sequence comprising applying a plurality of pulses including a first pulse and a second pulse having a different amplitude than the first pulse, measuring, for each pulse of the plurality of pulses, a series of sensor currents in response to the respective pulse, a first series of sensor currents being measured in response to the first pulse, a second series of sensor currents being measured in response to the second pulse; adjusting, via the processor, a slope value and an intercept value of a calibration for the biosensor system for a temperature effect; calculating, via the processor, a glucose concentration of the biological fluid sample using the adjusted calibration; and checking, via the processor, for underfill of the sample in the biosensor system, the checking including setting an error mode to "underfill" if a last measured current in the second series is less than a current threshold for underfill, or setting an error mode to "underfill" if the difference of a last measured current in the first series and a first measured current in the second series is greater than a current delta threshold for underfill. providing a biosensor system in form of an analytical instrument including a sensor strip having a sample interface on a base, the base forming a reservoir and a channel with an opening, the base further having a surface on which at least one working electrode and at least one counter electrode are disposed, the sample interface having conductors connected to the working electrode and the counter electrode, a biological fluid sample received in the opening flowing through the channel to fill at least in part the reservoir, and a measuring device in electrical or optical communication with the reservoir, the measurement device having electrical circuitry including a processor and a storage medium, the electrical circuitry being communicatively coupled to a signal generator and a sensor interface, the processor having instructions and data stored in the storage medium; detecting, by the biosensor, when a sample of a biological fluid is available for analysis, applying, via the processor, a pulse sequence to the biosensor system, the pulse sequence comprising applying a plurality of pulses including a first pulse and a second pulse having a different amplitude than the first pulse, measuring, for each pulse of the plurality of pulses, a series of sensor currents in response to the respective pulse, a first series of sensor currents being measured in response to the first pulse, a second series of sensor currents being measured in response to the second pulse; adjusting, via the processor, a slope value and an intercept value of a calibration for the biosensor system for a temperature effect; calculating, via the processor, a glucose concentration of the biological fluid sample using the adjusted calibration; and checking, via the processor, for underfill of the sample in the biosensor system, the checking including setting an error mode to "underfill" if a last measured current in the second series is less than a current threshold for underfill, or setting an error mode to "underfill" if the difference of a last measured current in the first series and a first measured current in the second series is greater than a current delta threshold for underfill.