A vacuum system for purification of and extraction of at least one substance from a liquid sample, characterized in that the vacuum system comprises: a rotatable base for rotation between at least a first position and a second position; at least one assay column assembly having at least a first assay column and at least one second assay column; at least one Stage 1 solvent reservoir having at least one easy connect fitting adapted for fluidic and reversible connection to the first assay column; at least one Stage 1 manifold mounted on a Stage 1 portion of the rotatable base having at least one easy connect fitting adapted for fluidic and reversible connection with the second assay column; at least one Stage 2 reservoir having at least one easy connect fitting adapted for fluidic and reversible connection with the second assay column; at least one Stage 2 manifold mounted on a Stage 2 portion of the rotatable base and having at least one easy connect fitting adapted for fluidic and reversible connection with the second assay column; wherein the first assay column has an easy connect fitting disposed at a first entry which is adapted for fluidic and reversible connection to the easy connect fitting of the Stage 1 solvent reservoir; wherein the first assay column has an easy connect fitting disposed at a first exit opposing the first entry of the first assay column which is adapted for fluidic and reversible connection to the second assay column; wherein the second assay column has an easy connect fitting disposed at a first entry of the second assay column which is adapted for fluidic and reversible selective alternate connection with the first assay column and the Stage 2 manifold; wherein the second assay column has an easy connect fitting disposed at a first exit opposing the first entry of the first assay column which is adapted for fluidic and reversible selective alternate connection with the Stage 1 manifold and the Stage 2 solvent reservoir, and optionally with a third assay column; and a vacuum pump adapted for selective fluidic coupling with the Stage 1 manifold and the Stage 2 manifold. a rotatable base for rotation between at least a first position and a second position; at least one assay column assembly having at least a first assay column and at least one second assay column; at least one Stage 1 solvent reservoir having at least one easy connect fitting adapted for fluidic and reversible connection to the first assay column; at least one Stage 1 manifold mounted on a Stage 1 portion of the rotatable base having at least one easy connect fitting adapted for fluidic and reversible connection with the second assay column; at least one Stage 2 reservoir having at least one easy connect fitting adapted for fluidic and reversible connection with the second assay column; at least one Stage 2 manifold mounted on a Stage 2 portion of the rotatable base and having at least one easy connect fitting adapted for fluidic and reversible connection with the second assay column; wherein the first assay column has an easy connect fitting disposed at a first entry which is adapted for fluidic and reversible connection to the easy connect fitting of the Stage 1 solvent reservoir; wherein the first assay column has an easy connect fitting disposed at a first exit opposing the first entry of the first assay column which is adapted for fluidic and reversible connection to the second assay column; wherein the second assay column has an easy connect fitting disposed at a first entry of the second assay column which is adapted for fluidic and reversible selective alternate connection with the first assay column and the Stage 2 manifold; wherein the second assay column has an easy connect fitting disposed at a first exit opposing the first entry of the first assay column which is adapted for fluidic and reversible selective alternate connection with the Stage 1 manifold and the Stage 2 solvent reservoir, and optionally with a third assay column; and a vacuum pump adapted for selective fluidic coupling with the Stage 1 manifold and the Stage 2 manifold.