An apparatus (100, 140, 300) for collection of human biological material comprising adipose and post-collection processing of collected material to prepare a fat graft comprising adipose, the apparatus (100, 140, 300) being orientable in a collection orientation, as oriented in the collection orientation the apparatus (100, 140, 300) comprising a container having an internal containment volume 330, the apparatus (100, 140, 300) characterized by: a filter (102, 152, 312); the internal containment volume comprising: a tissue retention volume (112, 148, 332) and a filtrate volume (113, 150, 334) separated by the filter (102, 152, 312); a collection volume within the filtrate volume (113, 150, 334), the collection volume having a bottom elevation corresponding to a bottom elevation of the filtrate volume (113, 150, 334) and a top elevation that is lower than the bottom elevation of the tissue retention volume (112, 148, 332); and a tapered portion that tapers in a downward direction with at least a portion of the tapered portion being located above the collection volume; an inlet port (114, 158, 304) in fluid communication with the tissue retention volume (112, 148, 332) and configured for introducing human biological material comprising adipose directly into the tissue retention volume (112, 148, 332); a suction port (118, 162, 302) in fluid communication with the filtrate volume (113, 150, 334) and providing access to the filtrate volume (113, 150, 334) for suctioning from the filtrate volume (113, 150, 334) components passing through the filter (102, 152, 312) from the tissue retention volume (112, 148, 332) to the filtrate volume (113, 150, 334); and a mixing device disposed at least in part within the tissue retention volume (112, 148, 332) for mixing contents within the tissue retention volume (112, 148, 332) and wherein the mixing device comprises: a rotatable shaft extending from outside of the internal containment volume to inside of the internal containment volume; and at least one mixing member disposed in the tissue retention volume (112, 148, 332) and connected with the shaft, wherein the at least one mixing member moves through the tissue retention volume (112, 148, 332) when the shaft is rotated. a filter (102, 152, 312); the internal containment volume comprising: a tissue retention volume (112, 148, 332) and a filtrate volume (113, 150, 334) separated by the filter (102, 152, 312); a collection volume within the filtrate volume (113, 150, 334), the collection volume having a bottom elevation corresponding to a bottom elevation of the filtrate volume (113, 150, 334) and a top elevation that is lower than the bottom elevation of the tissue retention volume (112, 148, 332); and a tapered portion that tapers in a downward direction with at least a portion of the tapered portion being located above the collection volume; a tissue retention volume (112, 148, 332) and a filtrate volume (113, 150, 334) separated by the filter (102, 152, 312); a collection volume within the filtrate volume (113, 150, 334), the collection volume having a bottom elevation corresponding to a bottom elevation of the filtrate volume (113, 150, 334) and a top elevation that is lower than the bottom elevation of the tissue retention volume (112, 148, 332); and a tapered portion that tapers in a downward direction with at least a portion of the tapered portion being located above the collection volume; an inlet port (114, 158, 304) in fluid communication with the tissue retention volume (112, 148, 332) and configured for introducing human biological material comprising adipose directly into the tissue retention volume (112, 148, 332); a suction port (118, 162, 302) in fluid communication with the filtrate volume (113, 150, 334) and providing access to the filtrate volume (113, 150, 334) for suctioning from the filtrate volume (113, 150, 334) components passing through the filter (102, 152, 312) from the tissue retention volume (112, 148, 332) to the filtrate volume (113, 150, 334); and a mixing device disposed at least in part within the tissue retention volume (112, 148, 332) for mixing contents within the tissue retention volume (112, 148, 332) and wherein the mixing device comprises: a rotatable shaft extending from outside of the internal containment volume to inside of the internal containment volume; and at least one mixing member disposed in the tissue retention volume (112, 148, 332) and connected with the shaft, wherein the at least one mixing member moves through the tissue retention volume (112, 148, 332) when the shaft is rotated. a rotatable shaft extending from outside of the internal containment volume to inside of the internal containment volume; and at least one mixing member disposed in the tissue retention volume (112, 148, 332) and connected with the shaft, wherein the at least one mixing member moves through the tissue retention volume (112, 148, 332) when the shaft is rotated.