A method for detecting one or more target nucleic acids of Hepatitis B Virus RNA in a sample, the method comprising: (a) using a nucleic acid sample for (b) performing an amplification step comprising contacting the nucleic acid sample with one or more competitive blocking oligonucleotide and one or more set of primers and a nucleic acid polymerase, wherein the one or more set of primers comprises one or more forward primer and one or more reverse transcription primer that also functions as a reverse primer, to produce an amplification product, if the one or more target nucleic acids of HBV RNA is present in the sample; (c) performing a hybridization step, comprising contacting the amplification product, if the one or more target nucleic acids of HBV RNA is present in the sample, with one or more probe; and (d) performing a detection step, comprising detecting the presence or absence of the amplification product, wherein the presence of the amplification product is indicative of the presence of the one or more target nucleic acids of HBV RNA in the sample, and wherein the absence of the amplification product is indicative of the absence of the one or more target nucleic acids of HBV RNA in the sample, wherein the one or more target nucleic acids of HBV RNA comprises a poly-A tail; wherein the one or more reverse transcription primer that also functions as a reverse primer comprises an HBV-specific sequence and further comprises a poly-T section to bind to the poly-A tail of the one or more target nucleic acids of HBV RNA; wherein the one or more competitive blocking oligonucleotide is non-extensible and hybridizes to any HBV DNA homologous to the HBV-specific sequence of the one or more reverse transcription primer that may be present in the sample, thereby preventing the binding of the one or more reverse transcription primer to any homologous HBV DNA that may be present in the sample. (a) using a nucleic acid sample for (b) performing an amplification step comprising contacting the nucleic acid sample with one or more competitive blocking oligonucleotide and one or more set of primers and a nucleic acid polymerase, wherein the one or more set of primers comprises one or more forward primer and one or more reverse transcription primer that also functions as a reverse primer, to produce an amplification product, if the one or more target nucleic acids of HBV RNA is present in the sample; (c) performing a hybridization step, comprising contacting the amplification product, if the one or more target nucleic acids of HBV RNA is present in the sample, with one or more probe; and (d) performing a detection step, comprising detecting the presence or absence of the amplification product, wherein the presence of the amplification product is indicative of the presence of the one or more target nucleic acids of HBV RNA in the sample, and wherein the absence of the amplification product is indicative of the absence of the one or more target nucleic acids of HBV RNA in the sample, wherein the one or more target nucleic acids of HBV RNA comprises a poly-A tail; wherein the one or more reverse transcription primer that also functions as a reverse primer comprises an HBV-specific sequence and further comprises a poly-T section to bind to the poly-A tail of the one or more target nucleic acids of HBV RNA; wherein the one or more competitive blocking oligonucleotide is non-extensible and hybridizes to any HBV DNA homologous to the HBV-specific sequence of the one or more reverse transcription primer that may be present in the sample, thereby preventing the binding of the one or more reverse transcription primer to any homologous HBV DNA that may be present in the sample.