Method for the enzymatic synthesis of an oligopeptide, comprising coupling i) an oligopeptide ester comprising 4 or more amino acid residues, - comprising at least two amino acid residues each with a side-chain functionality that is protected with a protecting group, and comprising an activated C-terminal ester represented by the formula C(=O)-O-CX 2 -C(=O)N-R 1 R 2, wherein each X independently represents a hydrogen atom or an alkyl group or an aryl group and R 1 represents a hydrogen atom or an alkyl group or an aryl group and R 2 represents a hydrogen atom or an alkyl group or an aryl group or an amino acid or a peptide residue with a C-terminal carboxyamide or carboxylic acid functionality, optionally protected on the side-chain functionality of the amino acid residue or on one or more of the side-chain functionalities of the peptide residue, - and wherein the oligopeptide ester optionally comprises N -terminal protection, with ii) an oligopeptide nucleophile comprising 4 or more amino acid residues, - comprising an N -terminal amine group, and at least two amino acid residues each with a side-chain functionality, that is protected with a protecting group and - wherein the oligopeptide nucleophile optionally comprises C-terminal protection, which coupling is carried out in an organic solvent or an organic solvent mixture comprising 0.1 vol% or less water relative to the total amount of liquids in which the coupling reaction predominantly takes place, in the presence of a subtilisin and wherein water that is released by the enzyme during the coupling reaction is removed. i) an oligopeptide ester comprising 4 or more amino acid residues, - comprising at least two amino acid residues each with a side-chain functionality that is protected with a protecting group, and comprising an activated C-terminal ester represented by the formula C(=O)-O-CX 2 -C(=O)N-R 1 R 2, wherein each X independently represents a hydrogen atom or an alkyl group or an aryl group and R 1 represents a hydrogen atom or an alkyl group or an aryl group and R 2 represents a hydrogen atom or an alkyl group or an aryl group or an amino acid or a peptide residue with a C-terminal carboxyamide or carboxylic acid functionality, optionally protected on the side-chain functionality of the amino acid residue or on one or more of the side-chain functionalities of the peptide residue, - and wherein the oligopeptide ester optionally comprises N -terminal protection, with - comprising at least two amino acid residues each with a side-chain functionality that is protected with a protecting group, and comprising an activated C-terminal ester represented by the formula C(=O)-O-CX 2 -C(=O)N-R 1 R 2, wherein each X independently represents a hydrogen atom or an alkyl group or an aryl group and R 1 represents a hydrogen atom or an alkyl group or an aryl group and R 2 represents a hydrogen atom or an alkyl group or an aryl group or an amino acid or a peptide residue with a C-terminal carboxyamide or carboxylic acid functionality, optionally protected on the side-chain functionality of the amino acid residue or on one or more of the side-chain functionalities of the peptide residue, - and wherein the oligopeptide ester optionally comprises N -terminal protection, ii) an oligopeptide nucleophile comprising 4 or more amino acid residues, - comprising an N -terminal amine group, and at least two amino acid residues each with a side-chain functionality, that is protected with a protecting group and - wherein the oligopeptide nucleophile optionally comprises C-terminal protection, which coupling is carried out in an organic solvent or an organic solvent mixture comprising 0.1 vol% or less water relative to the total amount of liquids in which the coupling reaction predominantly takes place, in the presence of a subtilisin and wherein water that is released by the enzyme during the coupling reaction is removed. - comprising an N -terminal amine group, and at least two amino acid residues each with a side-chain functionality, that is protected with a protecting group and - wherein the oligopeptide nucleophile optionally comprises C-terminal protection,