An apparatus for a controlled combination of at least one reagent with a culture media, the apparatus comprising: a bottle (400, 800) having a narrow upper portion (420, 620) and a lower chamber for receiving a biological sample wherein the lower chamber of the bottle (400, 800) contains a culture media; a tubular-shaped sealed chamber insert (320, 810) for receiving at least one reagent, the at least one reagent is selected from the group consisting of adsorption resin, lytic reagent, and a combination thereof, wherein the culture media is adapted to grow one of the group selected from aerobic microorganisms, anaerobic microorganisms, mycobacteria, fungi, or yeast and wherein the culture media is in liquid or lyophilized form; and a cap element (200, 760) threaded onto the narrow upper portion (420, 600) of the bottle wherein the cap element (200, 760) secures the tubular-shaped sealed chamber insert (320, 810) in the narrow upper portion of the bottle (400, 800), wherein the sealed chamber insert is configured to be disrupted by a tapered rod (110 700) having a proximal and distal end and fitted into the cap element (200, 700) when the cap element (200, 760) is twisted onto the bottle (400, 800), thereby advancing the tapered rod (110, 700) towards the sealed chamber insert, wherein the disruption of the sealed chamber insert (320, 810), by means of the distal end of the tapered rod piercing the seal chamber insert, allows the contents of the sealed chamber insert (320, 810) to flow into the lower chamber of the bottle (400, 800), wherein the lower chamber of the bottle comprises one or more sensors for monitoring conditions in the culture media or the headspace gas in the bottle. a bottle (400, 800) having a narrow upper portion (420, 620) and a lower chamber for receiving a biological sample wherein the lower chamber of the bottle (400, 800) contains a culture media; a tubular-shaped sealed chamber insert (320, 810) for receiving at least one reagent, the at least one reagent is selected from the group consisting of adsorption resin, lytic reagent, and a combination thereof, wherein the culture media is adapted to grow one of the group selected from aerobic microorganisms, anaerobic microorganisms, mycobacteria, fungi, or yeast and wherein the culture media is in liquid or lyophilized form; and a cap element (200, 760) threaded onto the narrow upper portion (420, 600) of the bottle wherein the cap element (200, 760) secures the tubular-shaped sealed chamber insert (320, 810) in the narrow upper portion of the bottle (400, 800), wherein the sealed chamber insert is configured to be disrupted by a tapered rod (110 700) having a proximal and distal end and fitted into the cap element (200, 700) when the cap element (200, 760) is twisted onto the bottle (400, 800), thereby advancing the tapered rod (110, 700) towards the sealed chamber insert, wherein the disruption of the sealed chamber insert (320, 810), by means of the distal end of the tapered rod piercing the seal chamber insert, allows the contents of the sealed chamber insert (320, 810) to flow into the lower chamber of the bottle (400, 800), wherein the lower chamber of the bottle comprises one or more sensors for monitoring conditions in the culture media or the headspace gas in the bottle.