A method for manufacturing an implantable micro-biosensor, comprising the steps of: a) providing a substrate having a first surface, wherein the first surface has a first sensing region to be implanted subcutaneously for measuring a physiological parameter of an analyte in a biological fluid; b) forming on the first surface of the substrate a first working electrode which at least includes a first sensing section, a material of the first sensing section including a first conductive material; c) forming on the first surface of the substrate at least one second working electrode which at least includes a second sensing section disposed proximate to at least one side of the first sensing section, a material of the second sensing section including a second conductive material different from the first conductive material, wherein both the second sensing section and the first sensing section are located at the first sensing region; and d) forming a chemical reagent layer which at least covers the first conductive material of the first sensing section and which reacts with the analyte to produce a product, steps b) and c) being implemented simultaneously by the sub-steps of: b1) applying the second conductive material on the first surface of the substrate; b2) subjecting the second conductive material to patterning so as to divide the second conductive material into a first area and at least one second area separated from each other; and b3) applying the first conductive material at the first sensing region to cover at least a portion of the second conductive material of the first area to form the first sensing section of the first working electrode and to configure the second conductive material at the second area as the at least one second working electrode, the sub-step of b2) permitting the second sensing section to be spaced apart from the at least one side of the first sensing section by a distance of up to 0.2 mm. a) providing a substrate having a first surface, wherein the first surface has a first sensing region to be implanted subcutaneously for measuring a physiological parameter of an analyte in a biological fluid; b) forming on the first surface of the substrate a first working electrode which at least includes a first sensing section, a material of the first sensing section including a first conductive material; c) forming on the first surface of the substrate at least one second working electrode which at least includes a second sensing section disposed proximate to at least one side of the first sensing section, a material of the second sensing section including a second conductive material different from the first conductive material, wherein both the second sensing section and the first sensing section are located at the first sensing region; and d) forming a chemical reagent layer which at least covers the first conductive material of the first sensing section and which reacts with the analyte to produce a product, b1) applying the second conductive material on the first surface of the substrate; b2) subjecting the second conductive material to patterning so as to divide the second conductive material into a first area and at least one second area separated from each other; and b3) applying the first conductive material at the first sensing region to cover at least a portion of the second conductive material of the first area to form the first sensing section of the first working electrode and to configure the second conductive material at the second area as the at least one second working electrode, the sub-step of b2) permitting the second sensing section to be spaced apart from the at least one side of the first sensing section by a distance of up to 0.2 mm.