A method for determining interaction between cells comprising: determining or receiving a sequence of images representing manipulating first cells, in a holding space, the holding space including a functionalized wall comprising second cells, the manipulating including settling of the first cells onto the functionalized wall and applying a force on the settled first cells; detecting groups of pixels representing first cells in first images representing the settling of the first cells onto the functionalized wall; and, tracking locations of detected first cells in the first images; and, determining settling events, a settling event being determined if a cell is no longer distinguishable from the background, wherein a cell is no longer distinguishable from the background when the classification of a group of pixels that is classified as a trackable cell changes into non-trackable, the location in the first image at which a cell settling event is detected defining a cell settling location; detecting groups of pixels representing first cells in second images captured during the application of the force and tracking locations of detected first cells, wherein tracked locations of a detected first cell in the second images form a tracking path, the first location of the tracking path defining a pop-up event, the location in a second image at which a pop-up event is detected defining a pop-up location; and, determining detachment events based on the settling locations and based on the pop-up locations, a detachment event defining a first cell being detached from a second cell due to application of the force on the first cell, and determining information about the interaction between first and second cells based on the force applied to the first cells. determining or receiving a sequence of images representing manipulating first cells, in a holding space, the holding space including a functionalized wall comprising second cells, the manipulating including settling of the first cells onto the functionalized wall and applying a force on the settled first cells; detecting groups of pixels representing first cells in first images representing the settling of the first cells onto the functionalized wall; and, tracking locations of detected first cells in the first images; and, determining settling events, a settling event being determined if a cell is no longer distinguishable from the background, wherein a cell is no longer distinguishable from the background when the classification of a group of pixels that is classified as a trackable cell changes into non-trackable, the location in the first image at which a cell settling event is detected defining a cell settling location; detecting groups of pixels representing first cells in second images captured during the application of the force and tracking locations of detected first cells, wherein tracked locations of a detected first cell in the second images form a tracking path, the first location of the tracking path defining a pop-up event, the location in a second image at which a pop-up event is detected defining a pop-up location; and, determining detachment events based on the settling locations and based on the pop-up locations, a detachment event defining a first cell being detached from a second cell due to application of the force on the first cell, and determining information about the interaction between first and second cells based on the force applied to the first cells.