A process for producing an optically active 2-alkyl-1,1,3-trialkoxycarbonylpropane represented by Formula: wherein, R 1, R 2 and R 3, which may be the same or different, represent a C1-C4 alkyl group, and * represents that the carbon atom is an asymmetrical center, comprising a step of asymmetric hydrolysis of an 2-alkyl-1,1,3-trialkoxycarbonylpropane represented by Formula: wherein, R 1, R 2 and R 3 are as defined above, by using an enzyme capable of selectively hydrolyzing an ester moiety of either one enantiomer of the 2-alkyl-1,1,3-trialkoxycarbonylpropane represented by Formula, wherein the enzyme is 1) a hydrolase originated from a microorganism of Candida or Bacillus, 2) an esterase or a lipase originated from Arthrobacter strain SC-6-98-28 (FERM BP-3658) or Chromobacterium strain SC-YM-1 (FERMBP-6703) or 3) a protein comprising an amino acid sequence selected from the following a) to d): a) an amino acid sequence represented by SEQ ID NO: 1 or 3; b) an amino acid sequence i) which is encoded by a nucleotide sequence of DNA having at least 90% homology to DNA having a nucleotide sequence represented by SEQ ID NO: 2 or 4, and ii) which is an amino acid sequence of a protein capable of selectively hydrolyzing an ester moiety of either one enantiomer of the 2-alkyl-1,1,3-trialkoxycarbonylpropane; c) an amino acid sequence i) in which one amino acid or two amino acids are deleted, replaced or added in the amino acid sequence represented by SEQ ID NO: 1 or 3, and ii) which is an amino acid sequence of a protein capable of selectively hydrolyzing an ester moiety of either one enantiomer of the 2-alkyl-1,1,3-trialkoxycarbonylpropane; and d) an amino acid sequence i) having at least 95% homology to the amino acid sequence represented by SEQ ID NO: 1 or 3, and ii) which is an amino acid sequence of a protein capable of selectively hydrolyzing an ester moiety of either one enantiomer of the 2-alkyl-1,1,3-trialkoxycarbonylpropane. 1) a hydrolase originated from a microorganism of Candida or Bacillus, 2) an esterase or a lipase originated from Arthrobacter strain SC-6-98-28 (FERM BP-3658) or Chromobacterium strain SC-YM-1 (FERMBP-6703) or 3) a protein comprising an amino acid sequence selected from the following a) to d): a) an amino acid sequence represented by SEQ ID NO: 1 or 3; b) an amino acid sequence i) which is encoded by a nucleotide sequence of DNA having at least 90% homology to DNA having a nucleotide sequence represented by SEQ ID NO: 2 or 4, and ii) which is an amino acid sequence of a protein capable of selectively hydrolyzing an ester moiety of either one enantiomer of the 2-alkyl-1,1,3-trialkoxycarbonylpropane; c) an amino acid sequence i) in which one amino acid or two amino acids are deleted, replaced or added in the amino acid sequence represented by SEQ ID NO: 1 or 3, and ii) which is an amino acid sequence of a protein capable of selectively hydrolyzing an ester moiety of either one enantiomer of the 2-alkyl-1,1,3-trialkoxycarbonylpropane; and d) an amino acid sequence i) having at least 95% homology to the amino acid sequence represented by SEQ ID NO: 1 or 3, and ii) which is an amino acid sequence of a protein capable of selectively hydrolyzing an ester moiety of either one enantiomer of the 2-alkyl-1,1,3-trialkoxycarbonylpropane. a) an amino acid sequence represented by SEQ ID NO: 1 or 3; b) an amino acid sequence i) which is encoded by a nucleotide sequence of DNA having at least 90% homology to DNA having a nucleotide sequence represented by SEQ ID NO: 2 or 4, and ii) which is an amino acid sequence of a protein capable of selectively hydrolyzing an ester moiety of either one enantiomer of the 2-alkyl-1,1,3-trialkoxycarbonylpropane; c) an amino acid sequence i) in which one amino acid or two amino acids are deleted, replaced or added in the amino acid sequence represented by SEQ ID NO: 1 or 3, and ii) which is an amino acid sequence of a protein capable of selectively hydrolyzing an ester moiety of either one enantiomer of the 2-alkyl-1,1,3-trialkoxycarbonylpropane; and d) an amino acid sequence i) having at least 95% homology to the amino acid sequence represented by SEQ ID NO: 1 or 3, and ii) which is an amino acid sequence of a protein capable of selectively hydrolyzing an ester moiety of either one enantiomer of the 2-alkyl-1,1,3-trialkoxycarbonylpropane.