A disposable cartridge comprising: a cartridge body with an inner cylindrical surface; a cylindrical insert disposed rotatably mounted within the inner cylindrical surface of the cartridge body, the cylindrical insert comprising: a disrupting chamber fluidly connected to a first port; at least a second chamber fluidly connected to a second port; first and second elongated channels disposed on opposite radial positions of a bottom surface of the cylindrical insert, each traversing at least a portion of the bottom surface of the cylindrical insert, the first elongated channel being fluidly isolated from the second elongated channel, the first elongated channel being fluidly connected to a first pair of channel ports disposed at a first radial position on the bottom surface of the cylindrical insert and the second elongated channel being fluidly connected to a second pair of channel ports disposed at a second radial position, opposite to the first radial position, on the bottom surface of the cylindrical insert; and an elongated washing channel that traverses at least a portion of the bottom surface of the cylindrical insert, the elongated washing channel comprising a first portion with a first width and a second portion with a second width that is greater than the first width, the second portion providing a location to concentrate magnetic nanoparticles during a washing step. a cartridge body with an inner cylindrical surface; a cylindrical insert disposed rotatably mounted within the inner cylindrical surface of the cartridge body, the cylindrical insert comprising: a disrupting chamber fluidly connected to a first port; at least a second chamber fluidly connected to a second port; first and second elongated channels disposed on opposite radial positions of a bottom surface of the cylindrical insert, each traversing at least a portion of the bottom surface of the cylindrical insert, the first elongated channel being fluidly isolated from the second elongated channel, the first elongated channel being fluidly connected to a first pair of channel ports disposed at a first radial position on the bottom surface of the cylindrical insert and the second elongated channel being fluidly connected to a second pair of channel ports disposed at a second radial position, opposite to the first radial position, on the bottom surface of the cylindrical insert; and an elongated washing channel that traverses at least a portion of the bottom surface of the cylindrical insert, the elongated washing channel comprising a first portion with a first width and a second portion with a second width that is greater than the first width, the second portion providing a location to concentrate magnetic nanoparticles during a washing step. a disrupting chamber fluidly connected to a first port; at least a second chamber fluidly connected to a second port; first and second elongated channels disposed on opposite radial positions of a bottom surface of the cylindrical insert, each traversing at least a portion of the bottom surface of the cylindrical insert, the first elongated channel being fluidly isolated from the second elongated channel, the first elongated channel being fluidly connected to a first pair of channel ports disposed at a first radial position on the bottom surface of the cylindrical insert and the second elongated channel being fluidly connected to a second pair of channel ports disposed at a second radial position, opposite to the first radial position, on the bottom surface of the cylindrical insert; and an elongated washing channel that traverses at least a portion of the bottom surface of the cylindrical insert, the elongated washing channel comprising a first portion with a first width and a second portion with a second width that is greater than the first width, the second portion providing a location to concentrate magnetic nanoparticles during a washing step.