A flow cell (110, 300, 400, 435, 475, 700, 802, 1110, 1300, 1500) for use in a microfluidic detection system, comprising: a flow cell body having a channel (412, 440, 600, 712, 860, 1010, 1060, 1350, 1410, 1420, 1430, 1440, 1510, 1520, 1530, 1540, 1550, 1560, 1570) extending along the flow cell body, the channel configured to convey a solution therethrough, wherein the flow cell body is configured to be removably held in the detection system; a bottom and a top surface provided on the flow cell body, the bottom or top surface being transparent and permitting light to pass therethrough; fluidic inlet and fluidic outlet ports provided on the flow cell body, the fluidic inlet and outlet ports being in fluid communication with the channel (412, 440, 600, 712, 860, 1010, 1060, 1350, 1410, 1420, 1440, 1450, 1460, 1470) within the flow cell body; a pump cavity (724, 830, 848, 930, 1224) provided in the flow cell body, the pump cavity fluidly communicating with, and being interposed between, an end of the channel and one of the fluidic inlet and outlet ports; and an electroosmotic pump held in the pump cavity (724, 830, 848, 930, 1224) within the flow cell body, the EO pump comprising electrodes (732, 734, 932, 934, 1232, 1234) and a porous membrane core, the porous membrane core extending across the pump cavity and substantially separating the pump cavity into first and second reservoirs, the EO pump (730, 833, 1015, 1015A, 1015B, 1055, 1233, 1333, 1580) inducing flow of the solution through the EO pump and channel (412, 440, 600, 712, 860, 1010, 1060, 1350, 1410, 1420, 1430, 1440, 1510, 1520, 1530, 1540, 1550, 1560, 1570) between the fluidic inlet and outlet ports, the EO pump comprising a vapor permeable membrane that allows gases generated by one of the electrodes (732, 734, 932, 934, 1232, 1234) to pass from the first reservoir to the second reservoir. a flow cell body having a channel (412, 440, 600, 712, 860, 1010, 1060, 1350, 1410, 1420, 1430, 1440, 1510, 1520, 1530, 1540, 1550, 1560, 1570) extending along the flow cell body, the channel configured to convey a solution therethrough, wherein the flow cell body is configured to be removably held in the detection system; a bottom and a top surface provided on the flow cell body, the bottom or top surface being transparent and permitting light to pass therethrough; fluidic inlet and fluidic outlet ports provided on the flow cell body, the fluidic inlet and outlet ports being in fluid communication with the channel (412, 440, 600, 712, 860, 1010, 1060, 1350, 1410, 1420, 1440, 1450, 1460, 1470) within the flow cell body; a pump cavity (724, 830, 848, 930, 1224) provided in the flow cell body, the pump cavity fluidly communicating with, and being interposed between, an end of the channel and one of the fluidic inlet and outlet ports; and an electroosmotic pump held in the pump cavity (724, 830, 848, 930, 1224) within the flow cell body, the EO pump comprising electrodes (732, 734, 932, 934, 1232, 1234) and a porous membrane core, the porous membrane core extending across the pump cavity and substantially separating the pump cavity into first and second reservoirs, the EO pump (730, 833, 1015, 1015A, 1015B, 1055, 1233, 1333, 1580) inducing flow of the solution through the EO pump and channel (412, 440, 600, 712, 860, 1010, 1060, 1350, 1410, 1420, 1430, 1440, 1510, 1520, 1530, 1540, 1550, 1560, 1570) between the fluidic inlet and outlet ports, the EO pump comprising a vapor permeable membrane that allows gases generated by one of the electrodes (732, 734, 932, 934, 1232, 1234) to pass from the first reservoir to the second reservoir.