A composition comprising a Signal Generating Complex, wherein the composition comprises: a pair of target probes (TPs), wherein a first TP of the pair of TPs comprises a nucleic acid sequence comprising two segments, (i) a segment comprising a binding site for a first segment of a target nucleic acid, and (ii) a segment comprising a binding site for a first base pre-pre-amplifier (base PPA); and wherein a second TP of the pair of TPs comprises a nucleic acid sequence comprising two segments, (i) a segment comprising a binding site for a second segment of the target nucleic acid, and (ii) a segment comprising a binding site for a second base PPA; a pair of PPAs comprising the first and second PPAs, wherein the first base PPA comprises a nucleic acid sequence comprising two segments, (i) a segment that binds to the first base PPA binding site of the first TP, and (ii) a segment comprising a plurality of first pre-amplifier binding segment repeats (first PA-BSRs), wherein the first PA-BSRs comprise two segments, (ia) a segment comprising a binding site for a first pre-amplifier (first PA) and (ib) a segment comprising a binding site for a second PA, wherein the segments of the first base PPA are in the order (i), (ia), (ib); and wherein the second base PPA comprises a nucleic acid sequence comprising two segments, (i) a segment that binds to the second base PPA binding site of the second TP, and (ii) a segment comprising a plurality of second PA-BSRs, wherein the second PA-BSRs comprise two segments, (iia) a segment comprising a binding site for the second PA and (iib) a segment comprising a binding site for the first PA, wherein the segments of the second base PPA are in the order (i), (iia), (iib); a plurality of the first PAs and the second PAs, wherein the first PAs comprise a nucleic acid sequence comprising three segments, (i) a segment complementary to the second PA-BSR, (ii) a segment complementary to the first PA-BSR, and (iii) a segment comprising a plurality of amplifier binding segment repeats (AMP-BSRs); and wherein the second PAs comprise a nucleic acid sequence comprising three segments, (i) a segment complementary to the first PA-BSR, (ii) a segment complementary to the second PA-BSR, and (iii) a segment comprising a plurality of amplifier binding segment repeats (AMP-BSRs); a plurality of amplifiers (AMPs), wherein the AMPs comprise a nucleic acid sequence comprising two segments, (i) a segment complementary to the AMP-BSRs, and (ii) a segment comprising a plurality of label probe binding segment repeats (LP-BSRs); and a plurality of label probes (LPs), wherein the LPs comprise a nucleic acid sequence comprising two segments, (i) a segment complementary to the LP-BSRs, and (ii) a segment that is detectable; wherein the respective binding sites and complementary segments are hybridized to form the SGC. a pair of target probes (TPs), wherein a first TP of the pair of TPs comprises a nucleic acid sequence comprising two segments, (i) a segment comprising a binding site for a first segment of a target nucleic acid, and (ii) a segment comprising a binding site for a first base pre-pre-amplifier (base PPA); and wherein a second TP of the pair of TPs comprises a nucleic acid sequence comprising two segments, (i) a segment comprising a binding site for a second segment of the target nucleic acid, and (ii) a segment comprising a binding site for a second base PPA; a pair of PPAs comprising the first and second PPAs, wherein the first base PPA comprises a nucleic acid sequence comprising two segments, (i) a segment that binds to the first base PPA binding site of the first TP, and (ii) a segment comprising a plurality of first pre-amplifier binding segment repeats (first PA-BSRs), wherein the first PA-BSRs comprise two segments, (ia) a segment comprising a binding site for a first pre-amplifier (first PA) and (ib) a segment comprising a binding site for a second PA, wherein the segments of the first base PPA are in the order (i), (ia), (ib); and wherein the second base PPA comprises a nucleic acid sequence comprising two segments, (i) a segment that binds to the second base PPA binding site of the second TP, and (ii) a segment comprising a plurality of second PA-BSRs, wherein the second PA-BSRs comprise two segments, (iia) a segment comprising a binding site for the second PA and (iib) a segment comprising a binding site for the first PA, wherein the segments of the second base PPA are in the order (i), (iia), (iib); a plurality of the first PAs and the second PAs, wherein the first PAs comprise a nucleic acid sequence comprising three segments, (i) a segment complementary to the second PA-BSR, (ii) a segment complementary to the first PA-BSR, and (iii) a segment comprising a plurality of amplifier binding segment repeats (AMP-BSRs); and wherein the second PAs comprise a nucleic acid sequence comprising three segments, (i) a segment complementary to the first PA-BSR, (ii) a segment complementary to the second PA-BSR, and (iii) a segment comprising a plurality of amplifier binding segment repeats (AMP-BSRs); a plurality of amplifiers (AMPs), wherein the AMPs comprise a nucleic acid sequence comprising two segments, (i) a segment complementary to the AMP-BSRs, and (ii) a segment comprising a plurality of label probe binding segment repeats (LP-BSRs); and a plurality of label probes (LPs), wherein the LPs comprise a nucleic acid sequence comprising two segments, (i) a segment complementary to the LP-BSRs, and (ii) a segment that is detectable;