A laser microscope comprising: an observation optical system with a laser light source, providing an observation light path for guiding an observation laser beam (L 1) emitted by the laser light source; an optical stimulation optical system with a laser light source, providing an optical stimulation light path for guiding an optical stimulation laser beam (L 2) emitted by the laser light source; a light-path combining unit (4; 30) configured to combine these laser light from the observation optical system and the optical stimulation optical system; and an objective lens for irradiating a specimen with the laser light combined by the light-path combining unit (4; 30) and for collecting light returning from the specimen, wherein the laser microscope further comprises, in at least the optical stimulation light path; at least one of a focal-position adjusting unit configured to adjust a focal position of the laser beam (L 2) in the optical-axis direction and a spot-diameter adjusting unit configured to adjust a spot diameter of the laser beam (L 2) emitted from the laser light source; an optical-axis misalignment detector configured to detect an amount of misalignment of an optical axis between the at least one of the focal-position adjusting unit and the spot-diameter adjusting unit, and the light-path combining unit (4; 30); and an alignment unit configured to adjust the optical axis of the laser beam (L 2) on the basis of the amount of misalignment of the optical axis of the laser beam (L 2) detected by the optical-axis misalignment detector so as to align the irradiation position of the optical stimulation laser beam to be coincident with the irradiation position of the observation laser beam. an observation optical system with a laser light source, providing an observation light path for guiding an observation laser beam (L 1) emitted by the laser light source; an optical stimulation optical system with a laser light source, providing an optical stimulation light path for guiding an optical stimulation laser beam (L 2) emitted by the laser light source; a light-path combining unit (4; 30) configured to combine these laser light from the observation optical system and the optical stimulation optical system; and an objective lens for irradiating a specimen with the laser light combined by the light-path combining unit (4; 30) and for collecting light returning from the specimen, wherein the laser microscope further comprises, in at least the optical stimulation light path; at least one of a focal-position adjusting unit configured to adjust a focal position of the laser beam (L 2) in the optical-axis direction and a spot-diameter adjusting unit configured to adjust a spot diameter of the laser beam (L 2) emitted from the laser light source; an optical-axis misalignment detector configured to detect an amount of misalignment of an optical axis between the at least one of the focal-position adjusting unit and the spot-diameter adjusting unit, and the light-path combining unit (4; 30); and an alignment unit configured to adjust the optical axis of the laser beam (L 2) on the basis of the amount of misalignment of the optical axis of the laser beam (L 2) detected by the optical-axis misalignment detector so as to align the irradiation position of the optical stimulation laser beam to be coincident with the irradiation position of the observation laser beam.