mhurhangee/ep-ind-claims huggingface dataset:
A method of isolating an industrial yeast strain that degrades L-asparagine under non-inducing conditions comprising: a) subculturing a wild-type industrial yeast strain, which expresses or has the capacity to express a cell-wall associated Asparaginase, in the presence of selective media; wherein the selective media contains D-asparagine as the sole nitrogen source; b) continuously subculturing, tracking growth rate and subjecting to physical or chemical mutagenesis weekly; c) selecting cultures of b) when the growth rate is comparable to growth rate on non-selective media; d) continuously subculturing cells in selective media containing methylamine, tracking growth rate, and mutagenizing weekly until growth rate in the presence of methylamine reaches that in selective media without methylamine; e) isolating individual colonies of d) by plating on selective media containing methylamine, growing said colonies and selecting large and fast growing colonies; f) assaying selected colonies of e) for the ability to degrade L-asparagine under non-inducing conditions and selecting at least one colony with high L-asparagine degradation activity, as compared to cells at the start of d); wherein non-inducing conditions are conditions in which extracellular L-asparagine degradation is repressed in wild-type yeast; g) repeating steps d) through f), increasing methylamine concentration each time, until L-asparagine degradation activity reaches a plateau; h) isolating the industrial yeast strain from g) in which L-asparagine degradation activity has reached a plateau. a) subculturing a wild-type industrial yeast strain, which expresses or has the capacity to express a cell-wall associated Asparaginase, in the presence of selective media; wherein the selective media contains D-asparagine as the sole nitrogen source; b) continuously subculturing, tracking growth rate and subjecting to physical or chemical mutagenesis weekly; c) selecting cultures of b) when the growth rate is comparable to growth rate on non-selective media; d) continuously subculturing cells in selective media containing methylamine, tracking growth rate, and mutagenizing weekly until growth rate in the presence of methylamine reaches that in selective media without methylamine; e) isolating individual colonies of d) by plating on selective media containing methylamine, growing said colonies and selecting large and fast growing colonies; f) assaying selected colonies of e) for the ability to degrade L-asparagine under non-inducing conditions and selecting at least one colony with high L-asparagine degradation activity, as compared to cells at the start of d); wherein non-inducing conditions are conditions in which extracellular L-asparagine degradation is repressed in wild-type yeast; g) repeating steps d) through f), increasing methylamine concentration each time, until L-asparagine degradation activity reaches a plateau; h) isolating the industrial yeast strain from g) in which L-asparagine degradation activity has reached a plateau.
A temperature sensitive ink composition comprising: a metal oxide nanoparticle; a binder; a solvent; an optional dispersant; and an optional surfactant; wherein the ink composition is a thermistor ink that exhibits a change in resistance which is dependent on temperature, wherein the binder is polyvinyl butyral, and wherein the solvent is selected from the group consisting of propylene glycol monomethyl ether acetate, 1-phenoxy-2-propanol, and combinations thereof. a metal oxide nanoparticle; a binder; a solvent; an optional dispersant; and an optional surfactant; wherein the ink composition is a thermistor ink that exhibits a change in resistance which is dependent on temperature, wherein the binder is polyvinyl butyral, and wherein the solvent is selected from the group consisting of propylene glycol monomethyl ether acetate, 1-phenoxy-2-propanol, and combinations thereof.
An agricultural combine comprising: a rotor; a concave extending about the rotor and defining a gap therebetween; at least one actuator (122, 124) coupled to the concave, wherein the at least one actuator (122, 124) is configured to change the gap when said at least one actuator (122, 124) is actuated; at least one force sensor configured to provide a force signal indicative of a force applied by the rotor and the concave to a crop mat disposed in the gap; at least one position sensor configured to provide a position signal indicative of a magnitude of the gap; an ECU coupled to the at least one actuator (122, 124), to the at least one force sensor and to the at least one position sensor, characterized in that the ECU contains at least one economic operating curve (154, 156, 158) that correlates a force applied to the crop mat and a magnitude of the gap, wherein the ECU is configured to read the force signal and the position signal, wherein the ECU is configured to actuate the at least one actuator (122, 124) to drive the force signal and the position signal to a point on the at least one economic operating curve (154, 156, 158). a rotor; a concave extending about the rotor and defining a gap therebetween; at least one actuator (122, 124) coupled to the concave, wherein the at least one actuator (122, 124) is configured to change the gap when said at least one actuator (122, 124) is actuated; at least one force sensor configured to provide a force signal indicative of a force applied by the rotor and the concave to a crop mat disposed in the gap; at least one position sensor configured to provide a position signal indicative of a magnitude of the gap; an ECU coupled to the at least one actuator (122, 124), to the at least one force sensor and to the at least one position sensor, characterized in that the ECU contains at least one economic operating curve (154, 156, 158) that correlates a force applied to the crop mat and a magnitude of the gap, wherein the ECU is configured to read the force signal and the position signal, wherein the ECU is configured to actuate the at least one actuator (122, 124) to drive the force signal and the position signal to a point on the at least one economic operating curve (154, 156, 158).
Method for determining the amount of binding of a cell interacting component to a cell surface structure present in a cell sample, comprising a) pre-labelling said cell interacting component with a first dye, b) incubating said component with desired cells to allow said component to bind to cell surface structures, c) washing away labelled cell interacting component not bound to the cells, d) lysing the cells to form a cell lysate, e) electrophoretic separation of proteins in said cell lysate in a gel, preferably a SDS PAGE gel, f) transferring proteins from the gel to a Western blotting membrane, g) Western blotting detection of housekeeping protein h) scanning or imaging of the membrane, and i) measuring signals from the pre-labelled cell interacting component and housekeeping protein. a) pre-labelling said cell interacting component with a first dye, b) incubating said component with desired cells to allow said component to bind to cell surface structures, c) washing away labelled cell interacting component not bound to the cells, d) lysing the cells to form a cell lysate, e) electrophoretic separation of proteins in said cell lysate in a gel, preferably a SDS PAGE gel, f) transferring proteins from the gel to a Western blotting membrane, g) Western blotting detection of housekeeping protein h) scanning or imaging of the membrane, and i) measuring signals from the pre-labelled cell interacting component and housekeeping protein.
A computer implemented method of processing data for use in additive manufacturing processes, the method comprising: receiving data representing a three dimensional object, the data comprising object property data indicative of at least one physical attribute of at least a portion of the three dimensional object; transforming the object property data into a plurality of device independent object property data objects, the object property data objects having a common data structure and each object property data object comprising a plurality of property values, wherein each value of the plurality of values is indicative of each of a predetermined set of object properties; and associating one of the device independent object property data objects with each of a plurality of locations within the object. receiving data representing a three dimensional object, the data comprising object property data indicative of at least one physical attribute of at least a portion of the three dimensional object; transforming the object property data into a plurality of device independent object property data objects, the object property data objects having a common data structure and each object property data object comprising a plurality of property values, wherein each value of the plurality of values is indicative of each of a predetermined set of object properties; and associating one of the device independent object property data objects with each of a plurality of locations within the object.
A radiation curable ink composition comprising: (a) at least one of a dendritic and a hyperbranched polyester acrylate component, a total amount of the dendritic and hyperbranched polyester acrylate consisting of 10-30% by weight of the ink composition; (b) an oligomer component including any of Urethane Acrylate, and Acrylic Oligomers, wherein the oligomer component is 1-10% by weight of the radiation curable ink; (c) a monomer component, wherein the monomer component is 52.8-68.1% by weight of the radiation curable ink composition and is comprised of a combination of mono-functional monomer and remainder di-functional monomer, wherein the mono-functional monomers include any of Tetrahydrofurfuryl Acrylate, Isobornyl Acrylate, and Vinylcaprolactam and comprise 85.2-94.4% of the monomer component, and the di-functional monomer is Propoxylated Neopentyl glycol diacrylate; (d) a photoinitiator component that is 12.5-15% by weight of the radiation curable ink; (e) an additive component including a stabilizer, polyacrylate, and Polyether Modified Polydimethyl Siloxane, wherein the additive component is 0.7% by weight of the radiation curable ink; and (f) a pigment component that is 2-2.9% by weight of the radiation curable ink composition. (a) at least one of a dendritic and a hyperbranched polyester acrylate component, a total amount of the dendritic and hyperbranched polyester acrylate consisting of 10-30% by weight of the ink composition; (b) an oligomer component including any of Urethane Acrylate, and Acrylic Oligomers, wherein the oligomer component is 1-10% by weight of the radiation curable ink; (c) a monomer component, wherein the monomer component is 52.8-68.1% by weight of the radiation curable ink composition and is comprised of a combination of mono-functional monomer and remainder di-functional monomer, wherein the mono-functional monomers include any of Tetrahydrofurfuryl Acrylate, Isobornyl Acrylate, and Vinylcaprolactam and comprise 85.2-94.4% of the monomer component, and the di-functional monomer is Propoxylated Neopentyl glycol diacrylate; (d) a photoinitiator component that is 12.5-15% by weight of the radiation curable ink; (e) an additive component including a stabilizer, polyacrylate, and Polyether Modified Polydimethyl Siloxane, wherein the additive component is 0.7% by weight of the radiation curable ink; and (f) a pigment component that is 2-2.9% by weight of the radiation curable ink composition.
A multi-phase measurement system for a multi-phase fluid, comprising: a reference value measurement assembly, which comprises at least one capillary and a capillary measurement device, wherein the inner cross-sectional area and the length of the capillary are dimensioned such that the phases of the multi-phase fluid separate in the flow direction when flowing through the capillary, and wherein the capillary measurement device is configured for determining at least one physical property of at least one of the phases flowing through the capillary; and a multi-phase measurement device, which is configured for measuring a volume and/or mass fraction of at least one phase of the multi-phase fluid and/or for measuring a volume and/or mass flow of at least one of the phases of the multi-phase fluid taking into account the physical property determined by the capillary measurement device, wherein the at least one physical property determined by the capillary measurement device allows a physical property to be inferred, on the basis of which the multi-phase measurement device determines the volume and/or mass fraction of at least one of the phases or the volume and/or mass flow of at least one of the phases. a reference value measurement assembly, which comprises at least one capillary and a capillary measurement device, wherein the inner cross-sectional area and the length of the capillary are dimensioned such that the phases of the multi-phase fluid separate in the flow direction when flowing through the capillary, and wherein the capillary measurement device is configured for determining at least one physical property of at least one of the phases flowing through the capillary; and a multi-phase measurement device, which is configured for measuring a volume and/or mass fraction of at least one phase of the multi-phase fluid and/or for measuring a volume and/or mass flow of at least one of the phases of the multi-phase fluid taking into account the physical property determined by the capillary measurement device, wherein the at least one physical property determined by the capillary measurement device allows a physical property to be inferred, on the basis of which the multi-phase measurement device determines the volume and/or mass fraction of at least one of the phases or the volume and/or mass flow of at least one of the phases.
A double-pocketed cyclone tube comprising: a cylindrical cyclone chamber, which includes interior chamber walls that slope inwards from a top of the cyclone chamber to a bottom of the cyclone chamber; a plurality of inlets extending from near the top of the cyclone chamber downwards and located on the exterior circumference of the cylindrical cyclone chamber, through which a substance may be introduced into the cylindrical cyclone chamber so as to create a rotational force sufficient to generate a cyclone in the cylindrical cyclone chamber, the double-pocketed cyclone tube characterized by: a plurality of T-shaped pocket separators, located at various angles around the circumference of the interior chamber walls, wherein each T-shaped pocket separator is positioned with a lower side of the T fixed to the interior chamber walls to form a double-pocket with two opposite-facing pocket separators, wherein the plurality of double pockets trap heavier material in the substance when in use, wherein forces generated by the cyclone will cause heavier material in the substance to move towards the interior chamber walls when in use. a cylindrical cyclone chamber, which includes interior chamber walls that slope inwards from a top of the cyclone chamber to a bottom of the cyclone chamber; a plurality of inlets extending from near the top of the cyclone chamber downwards and located on the exterior circumference of the cylindrical cyclone chamber, through which a substance may be introduced into the cylindrical cyclone chamber so as to create a rotational force sufficient to generate a cyclone in the cylindrical cyclone chamber, the double-pocketed cyclone tube characterized by: a plurality of T-shaped pocket separators, located at various angles around the circumference of the interior chamber walls, wherein each T-shaped pocket separator is positioned with a lower side of the T fixed to the interior chamber walls to form a double-pocket with two opposite-facing pocket separators, wherein the plurality of double pockets trap heavier material in the substance when in use, wherein forces generated by the cyclone will cause heavier material in the substance to move towards the interior chamber walls when in use.
Bicycle component for an at least partially muscle-powered bicycle having at least one shock absorber device having at least one damper device that can be controlled by at least one control device, wherein the bicycle component comprises at least one identification device having at least one sensor device and wherein the sensor device comprises at least one receiving unit for non-contact capturing of at least one signal, wherein at least part of the sensor device is pivotable with a steering movement and wherein the part of the sensor device or the entire sensor device may be attached to a stationary part of the bicycle component if transmission of the steering movement to the sensor device respectively to the pivotable part of the sensor device is ensured or wherein the sensor device can be disposed on at least one component of the bicycle which can, or will, pivot along with a steering movement and wherein the identification device is suitable and configured to identify a hazard in dependence on the captured signal and to send a suitable control signal to a central control device through which the at least one shock absorber device can be controlled in dependence on the identified hazard so that at least one damping characteristic of the shock absorber device can be adjusted by a signal from the identification device, characterized in that the sensor device comprises at least one transmitting unit for emitting at least one signal and that the receiving unit is suitable and configured to capture as a signal at least one reflection of the emitted signal returning from the hazard.
System for regulating a liquid in a circuit, with the system comprising: - a regulating valve comprising at least one inlet and one outlet and comprising a movable obturator the position of which makes it possible to adjust the flow rate of the liquid through the valve, - an expansion reservoir in communication with the liquid flowing in the circuit and intended to contain liquid and a compensating gas, the expansion reservoir being connected to the circuit by means of the valve and such that the expansion reservoir communicates with at least one from among the inlet and the outlet of the valve irrespective of the position of the obturator, the position of the obturator being independent of the pressure of the fluid in the expansion reservoir, with the obturator having at least one expansion channel and the system being arranged in such a way that the communication between the expansion reservoir and between one from among the inlet and the outlet of the valve is carried out at least partially by said expansion channel irrespective of the position of the obturator characterized in that the movable obturator is movable with respect to the expansion reservoir. - a regulating valve comprising at least one inlet and one outlet and comprising a movable obturator the position of which makes it possible to adjust the flow rate of the liquid through the valve, - an expansion reservoir in communication with the liquid flowing in the circuit and intended to contain liquid and a compensating gas,
An implant for reducing the size of a heart valve annulus, the implant comprising: a tubular body comprising a frame (920, 922, 1020) defining a proximal diameter and a distal diameter, the tubular body having a first structural configuration in which the proximal diameter is smaller than the distal diameter and a second structural configuration in which the proximal diameter is larger than the distal diameter; characterized by a plurality of helical anchors connected to the tubular body proximate the distal diameter of the frame, the plurality of helical anchors configured to be rotated by a plurality of rotatable drivers to advance the plurality of helical anchors distally relative to the frame (920, 922, 1020) and penetrate the heart valve annulus. a tubular body comprising a frame (920, 922, 1020) defining a proximal diameter and a distal diameter, the tubular body having a first structural configuration in which the proximal diameter is smaller than the distal diameter and a second structural configuration in which the proximal diameter is larger than the distal diameter; characterized by a plurality of helical anchors connected to the tubular body proximate the distal diameter of the frame, the plurality of helical anchors configured to be rotated by a plurality of rotatable drivers to advance the plurality of helical anchors distally relative to the frame (920, 922, 1020) and penetrate the heart valve annulus.
A method of processing user interaction in a medical environment with a medical image for producing measurement data related to graphics on the medical image, wherein the graphics on the medical image comprises at least one manually or automatically created graphic object (40;50,51;60) from which the measurement data is derived, the medical image, the at least one graphic object and the measurement data being displayed on graphics display means (10; 103), said method comprising the step of, responsive to user input comprising at least one position, attaching at least one measurement object (42;52;61,62) to said graphic object, wherein the measurement object comprises a measurement label (42;52;61) for display of a value representing measurement data which is derived from the graphic object, characterized in that the measurement object (42;52;61,62) is moved in response to a user interaction between different graphic objects supporting the same measurement type, and the measurement object is automatically updated (52;61) with the current measurement data of the graphic object that it is currently attached to.
An apparatus for collecting fluid from a tissue site, comprising: an upstream layer having a hydrophilic side and a hydrophobic side; a downstream layer having a hydrophilic side and a hydrophobic side; an absorbent member positioned between the upstream layer and the downstream layer, the hydrophilic side of the upstream layer positioned facing the absorbent member, and the hydrophilic side of the downstream layer positioned facing the absorbent member; a sealing member positioned adjacent to the downstream layer; a non-adherent interface adapted to be positioned between the upstream layer and the tissue site; and wherein the sealing member is bonded to the downstream layer by a first hot-melt web layer, and the non-adherent interface is bonded to the upstream layer by a second hot-melt web layer. an upstream layer having a hydrophilic side and a hydrophobic side; a downstream layer having a hydrophilic side and a hydrophobic side; an absorbent member positioned between the upstream layer and the downstream layer, the hydrophilic side of the upstream layer positioned facing the absorbent member, and the hydrophilic side of the downstream layer positioned facing the absorbent member; a sealing member positioned adjacent to the downstream layer; a non-adherent interface adapted to be positioned between the upstream layer and the tissue site; and wherein the sealing member is bonded to the downstream layer by a first hot-melt web layer, and the non-adherent interface is bonded to the upstream layer by a second hot-melt web layer.