A method for observing a light emission (14, 15) of a sample in a medium with a refractive index n L, the sample being arranged on a surface of a transparent support of refractive index n s, which is greater than n L, the light emission comprising luminous components of a given amplitude and phase, oriented toward the support and forming an angle θ with a direction perpendicular to the surface, among which, on the one hand, supercritical luminous components, for which the angle θ is strictly greater than a critical angle θ c = arcsin(n L /n s) and on the other hand, critical or subcritical luminous components, for which the angle θ is less than or equal to the critical angle θ c, the method implementing an observation device capable of: - capturing at least part of the light emission from a region of interest of the sample and obtaining a captured luminous signal comprising luminous components arising from the supercritical luminous components of the light emission; - applying filters to the captured luminous signal in order to selectively decrease the amplitude and/or change the phase of some of the luminous components of the captured luminous signal to obtain a filtered light signal; and - transforming the filtered luminous signal into an image zone of the region of interest of the sample; the method being characterized in that: - it realizes a modulation of the filtered luminous signal, in which luminous components arising from the critical or subcritical luminous components of the light emission are allowed to pass through in order to obtain image zones (6a, 6b) of one and the same region of interest of the sample, the modulation being applied to all or some of the luminous components of the captured luminous signal arising from the supercritical luminous components of the light emission; and - at least one useful image zone of the sample is produced by combining the image zones (6a, 6b), the combination evidencing differences between the image zones (6a, 6b) associated with the modulation. - capturing at least part of the light emission from a region of interest of the sample and obtaining a captured luminous signal comprising luminous components arising from the supercritical luminous components of the light emission; - applying filters to the captured luminous signal in order to selectively decrease the amplitude and/or change the phase of some of the luminous components of the captured luminous signal to obtain a filtered light signal; and - transforming the filtered luminous signal into an image zone of the region of interest of the sample; - it realizes a modulation of the filtered luminous signal, in which luminous components arising from the critical or subcritical luminous components of the light emission are allowed to pass through in order to obtain image zones (6a, 6b) of one and the same region of interest of the sample, the modulation being applied to all or some of the luminous components of the captured luminous signal arising from the supercritical luminous components of the light emission; and - at least one useful image zone of the sample is produced by combining the image zones (6a, 6b), the combination evidencing differences between the image zones (6a, 6b) associated with the modulation.