A method of producing an immunoglobulin G Fc region binding polypeptide, comprising the amino acid sequence: i) X 1 -([spacer1]-KFDKEQQN AFYEILX 17 LPX 20 LTEEQRNAFI QKLKDAPSQS AELLAEAKQL X 51 EAQA-[spacer2]) n -C CTERM-1 X CTERM wherein, independently of each other, X 1 is P; [spacer1] is an amino acid sequence consisting of 1-3 amino acid residues; X 17 is any amino acid residue; X 20 is any amino acid residue; X 51 is any amino acid residue; [spacer2] is an amino acid sequence consisting of 0-20 amino acid residues; X CTERM is D; and n is 1-4; said method comprising: a) producing a multimer polypeptide comprising at least two subunits, each subunit being said immunoglobulin G Fc region binding polypeptide, and an N-terminal leader peptide comprising a D residue as the last residue thereof, by - providing a host cell harboring an expression vector comprising a polynucleotide comprising a nucleotide sequence encoding said multimer polypeptide, - culturing the host cell under conditions that enable the expression of said polynucleotide into a multimer polypeptide, and - isolating the resulting multimer polypeptide, b) cleaving the multimer polypeptide at sites comprising the sequence DP using acid-catalyzed hydrolysis, yielding said immunoglobulin G Fc region binding polypeptides and N-terminal leader polypeptide, and c) isolating said immunoglobulin G Fc region binding polypeptide. i) X 1 -([spacer1]-KFDKEQQN AFYEILX 17 LPX 20 LTEEQRNAFI QKLKDAPSQS AELLAEAKQL X 51 EAQA-[spacer2]) n -C CTERM-1 X CTERM wherein, independently of each other, X 1 is P; [spacer1] is an amino acid sequence consisting of 1-3 amino acid residues; X 17 is any amino acid residue; X 20 is any amino acid residue; X 51 is any amino acid residue; [spacer2] is an amino acid sequence consisting of 0-20 amino acid residues; X CTERM is D; and n is 1-4; said method comprising: a) producing a multimer polypeptide comprising at least two subunits, each subunit being said immunoglobulin G Fc region binding polypeptide, and an N-terminal leader peptide comprising a D residue as the last residue thereof, by - providing a host cell harboring an expression vector comprising a polynucleotide comprising a nucleotide sequence encoding said multimer polypeptide, - culturing the host cell under conditions that enable the expression of said polynucleotide into a multimer polypeptide, and - isolating the resulting multimer polypeptide, b) cleaving the multimer polypeptide at sites comprising the sequence DP using acid-catalyzed hydrolysis, yielding said immunoglobulin G Fc region binding polypeptides and N-terminal leader polypeptide, and c) isolating said immunoglobulin G Fc region binding polypeptide. a) producing a multimer polypeptide comprising at least two subunits, each subunit being said immunoglobulin G Fc region binding polypeptide, and an N-terminal leader peptide comprising a D residue as the last residue thereof, by - providing a host cell harboring an expression vector comprising a polynucleotide comprising a nucleotide sequence encoding said multimer polypeptide, - culturing the host cell under conditions that enable the expression of said polynucleotide into a multimer polypeptide, and - isolating the resulting multimer polypeptide, - providing a host cell harboring an expression vector comprising a polynucleotide comprising a nucleotide sequence encoding said multimer polypeptide, - culturing the host cell under conditions that enable the expression of said polynucleotide into a multimer polypeptide, and - isolating the resulting multimer polypeptide, b) cleaving the multimer polypeptide at sites comprising the sequence DP using acid-catalyzed hydrolysis, yielding said immunoglobulin G Fc region binding polypeptides and N-terminal leader polypeptide, and c) isolating said immunoglobulin G Fc region binding polypeptide.