A system for identifying objects of interest in a biological specimen, the objects of interest being identified from normal cells and background areas of the biological specimen, the system comprising: a processor; memory; and computer instructions stored in memory and executable by the processor for performing the functions of: obtaining an image of the biological specimen, the image of the biological specimen comprising pixels, each of the pixels being defined by a first component, a second component, and a third component; forming a complement of the first component, the second component, and the third component for each of the pixels, wherein the complement of the first component, the second component, and the third component for each of the pixels are calculated by subtracting the first component, the second component, and the third component respectively from a maximum component level; transforming the image of the biological specimen to produce a transformed image, the image of the biological specimen being transformed by calculating for each of the pixels a transform value, the transform value being defined by a square root of p 1 2 + p 2 2 + p 3 2 × p 1 ⁢ c 2 + p 2 ⁢ c 2 + p 3 ⁢ c 2 - p 1 × p 1 ⁢ c + p 2 × p 2 ⁢ c + p 3 × p 3 ⁢ c 2, wherein p 1, p 2, and p 3, are a complement of the first component, the second component, and the third component, respectively, of a pixel to be transformed and p 1c, p 2c, and p 3c are the complement of the first component, second component, and third component, respectively, of a representative counterstained pixel, whereby the transformed image assists in identifying the objects of interest, if any, in the biological specimen. a processor; memory; and computer instructions stored in memory and executable by the processor for performing the functions of: obtaining an image of the biological specimen, the image of the biological specimen comprising pixels, each of the pixels being defined by a first component, a second component, and a third component; forming a complement of the first component, the second component, and the third component for each of the pixels, wherein the complement of the first component, the second component, and the third component for each of the pixels are calculated by subtracting the first component, the second component, and the third component respectively from a maximum component level; transforming the image of the biological specimen to produce a transformed image, the image of the biological specimen being transformed by calculating for each of the pixels a transform value, the transform value being defined by a square root of p 1 2 + p 2 2 + p 3 2 × p 1 ⁢ c 2 + p 2 ⁢ c 2 + p 3 ⁢ c 2 - p 1 × p 1 ⁢ c + p 2 × p 2 ⁢ c + p 3 × p 3 ⁢ c 2, wherein p 1, p 2, and p 3, are a complement of the first component, the second component, and the third component, respectively, of a pixel to be transformed and p 1c, p 2c, and p 3c are the complement of the first component, second component, and third component, respectively, of a representative counterstained pixel, whereby the transformed image assists in identifying the objects of interest, if any, in the biological specimen. obtaining an image of the biological specimen, the image of the biological specimen comprising pixels, each of the pixels being defined by a first component, a second component, and a third component; forming a complement of the first component, the second component, and the third component for each of the pixels, wherein the complement of the first component, the second component, and the third component for each of the pixels are calculated by subtracting the first component, the second component, and the third component respectively from a maximum component level; transforming the image of the biological specimen to produce a transformed image, the image of the biological specimen being transformed by calculating for each of the pixels a transform value, the transform value being defined by a square root of p 1 2 + p 2 2 + p 3 2 × p 1 ⁢ c 2 + p 2 ⁢ c 2 + p 3 ⁢ c 2 - p 1 × p 1 ⁢ c + p 2 × p 2 ⁢ c + p 3 × p 3 ⁢ c 2, wherein p 1, p 2, and p 3, are a complement of the first component, the second component, and the third component, respectively, of a pixel to be transformed and p 1c, p 2c, and p 3c are the complement of the first component, second component, and third component, respectively, of a representative counterstained pixel, whereby the transformed image assists in identifying the objects of interest, if any, in the biological specimen.