A method for producing an optically active nipecotamide derivative or an optically active nipecotin derivative, comprising steps of: stereoselectively hydrolyzing a racemic nipecotamide derivative represented by the following formula: wherein, R 1 represents a hydrogen atom or a benzyl group, by using an enzyme source having an ability to stereoselectively hydrolyze the racemic nipecotamide derivative, to convert the racemic nipecotamide derivative into a mixture of an optically active nipecotamide derivative represented by the following formula: wherein. * indicates an asymmetric carbon atom; and R 1 means the same as the above, and an optically active nipecotin derivative represented by the following formula: wherein, * and R 1 mean the same as the above; and then isolating the optically active nipecotamide derivative or the optically active nipecotin derivative from the mixture; wherein the enzyme source is derived from a microorganism selected from the group consisting of Achromobacter xylosoxidans subsp. xylosoxidans and Cupriavidus sp., and an S-enantiomer in the racemic nipecotamide derivative is stereoselectively hydrolyzed to produce an R-enantiomer of the optically active nipecotamide derivative or an S-enantiomer of the optically active nipecotin derivative, or the enzyme source is derived from a microorganism seletected from the group consisting of Pectobacterium carotovorum subsp. carotovorum and Staphylococcus epidermidis, and an R-enantiomer in the racemic nipecotamide derivative is stereoselectively hydrolyzed to produce an S-enantiomer of the optically active nipecotamide derivative or an R-enantiomer of the optically active nipecotin derivative. stereoselectively hydrolyzing a racemic nipecotamide derivative represented by the following formula: wherein, R 1 represents a hydrogen atom or a benzyl group, by using an enzyme source having an ability to stereoselectively hydrolyze the racemic nipecotamide derivative, to convert the racemic nipecotamide derivative into a mixture of an optically active nipecotamide derivative represented by the following formula: wherein. * indicates an asymmetric carbon atom; and R 1 means the same as the above, and an optically active nipecotin derivative represented by the following formula: wherein, * and R 1 mean the same as the above; and then isolating the optically active nipecotamide derivative or the optically active nipecotin derivative from the mixture; wherein the enzyme source is derived from a microorganism selected from the group consisting of Achromobacter xylosoxidans subsp. xylosoxidans and Cupriavidus sp., and an S-enantiomer in the racemic nipecotamide derivative is stereoselectively hydrolyzed to produce an R-enantiomer of the optically active nipecotamide derivative or an S-enantiomer of the optically active nipecotin derivative, or the enzyme source is derived from a microorganism seletected from the group consisting of Pectobacterium carotovorum subsp. carotovorum and Staphylococcus epidermidis, and an R-enantiomer in the racemic nipecotamide derivative is stereoselectively hydrolyzed to produce an S-enantiomer of the optically active nipecotamide derivative or an R-enantiomer of the optically active nipecotin derivative.