A system, comprising: first and second stains, wherein the first and second stains are counterstains that are configured to bind to a sample without molecular specificity, and which have similar spectral absorption and emission profiles, wherein absorption profiles are similar if, at the peak absorption wavelength of the first stain, the absorption value of the second stain is at least 30% or more of the peak absorption value of the second stain, and wherein emission profiles are similar if, at the peak emission wavelength of the first stain, the emission value of the second stain is at least 30% or more of the peak emission value of the second stain; a detector configured to obtain multiple images of a sample stained with the first and second stains; and an electronic processor configured to: decompose the multiple images into an unmixed image set, wherein the unmixed image set comprises a first unmixed image corresponding to the first stain and a second unmixed image corresponding to the second stain; and identify nuclear regions in the sample based on the first unmixed image and identify cytoplasm regions in the sample based on the second unmixed image. first and second stains, wherein the first and second stains are counterstains that are configured to bind to a sample without molecular specificity, and which have similar spectral absorption and emission profiles, wherein absorption profiles are similar if, at the peak absorption wavelength of the first stain, the absorption value of the second stain is at least 30% or more of the peak absorption value of the second stain, and wherein emission profiles are similar if, at the peak emission wavelength of the first stain, the emission value of the second stain is at least 30% or more of the peak emission value of the second stain; wherein the first and second stains are counterstains that are configured to bind to a sample without molecular specificity, and which have similar spectral absorption and emission profiles, wherein absorption profiles are similar if, at the peak absorption wavelength of the first stain, the absorption value of the second stain is at least 30% or more of the peak absorption value of the second stain, and wherein emission profiles are similar if, at the peak emission wavelength of the first stain, the emission value of the second stain is at least 30% or more of the peak emission value of the second stain; a detector configured to obtain multiple images of a sample stained with the first and second stains; and an electronic processor configured to: decompose the multiple images into an unmixed image set, wherein the unmixed image set comprises a first unmixed image corresponding to the first stain and a second unmixed image corresponding to the second stain; and identify nuclear regions in the sample based on the first unmixed image and identify cytoplasm regions in the sample based on the second unmixed image. decompose the multiple images into an unmixed image set, wherein the unmixed image set comprises a first unmixed image corresponding to the first stain and a second unmixed image corresponding to the second stain; and identify nuclear regions in the sample based on the first unmixed image and identify cytoplasm regions in the sample based on the second unmixed image.