mhurhangee/ep-ind-claims huggingface dataset:
An in vitro method of identifying an epitope on a protein that can be bound by an antibody, said method comprising: (i) performing limited or restricted proteolysis on said protein using a single first protease or a combination of first proteases; (ii) performing non-limited proteolysis or performing limited or restricted proteolysis on said protein using a single second protease or a combination of second proteases, wherein the specificity(ies) of said second protease(s) are all different from the specificity(ies) of the protease(s) used in step (i); (iii) analysing peptides which are released from said protein in step (ii) to identify peptides in which one end has been cut by a said first protease and the other end has been cut by a said second protease; (iv) probing one or more epitopes in a region of the protein containing or flanking a cut site for a said first protease as identified in step (iii) with one or more antibodies directed to said epitopes, thereby identifying one or more epitopes on the protein that can be bound by an antibody; wherein said method further comprises a step prior to step (iv) of generating one or more isolated epitopes having sequences that correspond to one or more epitopes on said protein that are in a region of the protein containing or flanking a cut site for a said first protease as identified in step (iii), and generating antibodies that are directed to said isolated epitopes, and using said antibodies in step (iv) for probing one or more epitopes on said protein. (i) performing limited or restricted proteolysis on said protein using a single first protease or a combination of first proteases; (ii) performing non-limited proteolysis or performing limited or restricted proteolysis on said protein using a single second protease or a combination of second proteases, wherein the specificity(ies) of said second protease(s) are all different from the specificity(ies) of the protease(s) used in step (i); (iii) analysing peptides which are released from said protein in step (ii) to identify peptides in which one end has been cut by a said first protease and the other end has been cut by a said second protease; (iv) probing one or more epitopes in a region of the protein containing or flanking a cut site for a said first protease as identified in step (iii) with one or more antibodies directed to said epitopes, thereby identifying one or more epitopes on the protein that can be bound by an antibody; wherein said method further comprises a step prior to step (iv) of generating one or more isolated epitopes having sequences that correspond to one or more epitopes on said protein that are in a region of the protein containing or flanking a cut site for a said first protease as identified in step (iii), and generating antibodies that are directed to said isolated epitopes, and using said antibodies in step (iv) for probing one or more epitopes on said protein.
Actuating unit for actuating a flushing device, comprising a bearing plate, at least one actuating element that is movable relative to the bearing plate, and at least one bearing element that is formed in a sprung manner and made of a spring-elastic material, said bearing element being connected to the bearing plate by a first end and to the at least one actuating element by a second end, wherein the bearing element is connected to the bearing plate and/or the actuating element by at least one of the two ends (5, 6) via a form-fitting and force-fitting connection, characterized in that the bearing element is connectable to the bearing plate and the actuating element in a plug-in direction, wherein the bearing element and/or the bearing plate and the actuating element, respectively, have at least one stop element which is formed in a sprung manner and prevents any movement counter to the plug-in direction, wherein the plug-in direction is oriented at right angles to the rear surface of the bearing plate.
A replication incompetent recombinant retroviral particle comprising a retroviral genome comprising one or more nucleic acid sequences operatively linked to a promoter active in T cells, wherein: A. a first nucleic acid sequence of the one or more nucleic acid sequences encodes two or more inhibitory RNA molecules directed against one or more RNA targets, and B. a second nucleic acid sequence of the one or more nucleic acid sequences encodes a chimeric antigen receptor comprising an antigen-specific targeting region (ASTR), a transmembrane domain, and an intracellular activating domain, wherein the replication incompetent recombinant retroviral particle comprises on its surface one or more pseudotyping elements, wherein the replication incompetent recombinant retroviral particle further comprises: (i) an activation element on its surface, wherein the activation element is fused to a membrane attachment sequence, and wherein the activation element is a polypeptide capable of binding to CD3, and/or (ii) a third nucleic acid sequence of the one or more nucleic acid sequences, wherein the third nucleic acid sequence encodes at least one lymphoproliferative element that is not an inhibitory RNA molecule. A. a first nucleic acid sequence of the one or more nucleic acid sequences encodes two or more inhibitory RNA molecules directed against one or more RNA targets, and B. a second nucleic acid sequence of the one or more nucleic acid sequences encodes a chimeric antigen receptor comprising an antigen-specific targeting region (ASTR), a transmembrane domain, and an intracellular activating domain, wherein the replication incompetent recombinant retroviral particle comprises on its surface one or more pseudotyping elements,
A method for controlling a communications system, the method being implemented in a base station, the base station comprising a General Packet Multiplexer that is configured for coordinating an overall delivery of voice over internet protocol packets and data packets, the base station further comprising a voice over internet protocol, VoIP, packet multiplexer that is configured to for controlling VoIP traffic, and, the base station further comprising a data user scheduler that is configured for providing a data scheduling function for general packet data services, the method comprising: receiving, at the VoIP packet multiplexer, a plurality of voice over internet protocol packets; accumulating, at the VoIP packet multiplexer the plurality of the voice over internet protocol packets; providing, by the VoIP packet multiplexer, the accumulated plurality of the voice over internet protocol packets to the General Packet Multiplexer; combining, at the General Packet Multiplexer, the VoIP packets and data packets into a format suitable for physical layer transmission, the combination of the packets to be transmitted by a Physical Packet Transmitter; and transmitting, by the Physical Packet Transmitter, the accumulated plurality of voice over internet protocol packets within a single time slot. receiving, at the VoIP packet multiplexer, a plurality of voice over internet protocol packets; accumulating, at the VoIP packet multiplexer the plurality of the voice over internet protocol packets; providing, by the VoIP packet multiplexer, the accumulated plurality of the voice over internet protocol packets to the General Packet Multiplexer; combining, at the General Packet Multiplexer, the VoIP packets and data packets into a format suitable for physical layer transmission, the combination of the packets to be transmitted by a Physical Packet Transmitter; and transmitting, by the Physical Packet Transmitter, the accumulated plurality of voice over internet protocol packets within a single time slot.
An antibody-drug conjugate directed against uPARAP comprising: a) an antibody which binds to the amino acid sequence of SEQ ID NO: 32 or 33 (the Fibronectin type II (FN-II) domain of uPARAP), b) an active agent, wherein the active agent is selected from a therapeutic agent, a cytotoxic agent, a radioisotope and a detectable label, and c) a linker which links a) to b). a) an antibody which binds to the amino acid sequence of SEQ ID NO: 32 or 33 (the Fibronectin type II (FN-II) domain of uPARAP), b) an active agent, wherein the active agent is selected from a therapeutic agent, a cytotoxic agent, a radioisotope and a detectable label, and c) a linker which links a) to b).
A flame-retardant sheet or film having a thickness of 0.01 to 0.22 mm, which is molded from a polycarbonate resin composition containing, relative to 100 parts by mass of a polycarbonate resin, 7 to 30 parts by mass of a flame retardant comprising a phosphorus-containing flame retardant represented by the following general formula or or, wherein the thickness distribution at the time of sheet or film molding in the direction perpendicular to the direction of resin flow is controlled to be within ± 10% of the average thickness: [in formula, R 1, R 2, R 3 and R 4 each represent an alkyl group containing 1 to 6 carbon atoms or an aryl group containing 6 to 20 carbon atoms which may be substituted with an alkyl group, p, q, r and s are each 0 or 1, k is an integer of 1 to 5, and X 1 represents an arylene group]; [in formula, t is an integer of 3 to 25, and R 5 and R 6, which may be the same or different, each represent an aryl group or an alkylaryl group]; or [in formula, u is an integer of 3 to 10,000, R 9 represents at least one selected from a -N=P(OR 7) 3 group, a -N=P(OR 8) 3 group, a -N=P OR 7 group and a -N=P OR 8 group, and R 10 represents at least one selected from a -P(OR 7) 4 group, a -P(OR 8) 4 group, a -P (OR 7) 2 group and a -P (OR 8) 2 group, wherein R 7 and R 8, which may be the same or different, each represent an aryl group or an alkylaryl group].
An electrodischarge machining method which uses an electrodischarge machine which is provided with a columnar or cylindrical shaped electrode so as to form a through hole which has a straight part and an inclined part connected with the straight part in a workpiece, comprising: an inclined part machining process which arranges the electrode so that an axis of the electrode extends in a depth direction of the through hole while making the electrode move relative to the workpiece and uses electrodischarge machining to form the inclined part; and a straight part machining process which arranges the electrode so that the axis extends in the depth direction of the through hole while making the electrode move relative to the workpiece and uses electrodischarge machining to form the straight part; and characterized by further comprising: an inside wall surface machining process which arranges the electrode so that the axis of the electrode becomes vertical to an inside wall surface of the inclined part which is formed by an inclined part machining process while making the electrode move relative to the workpiece to machine the inside wall surface of the inclined part by electrodischarge machining. an inclined part machining process which arranges the electrode so that an axis of the electrode extends in a depth direction of the through hole while making the electrode move relative to the workpiece and uses electrodischarge machining to form the inclined part; and a straight part machining process which arranges the electrode so that the axis extends in the depth direction of the through hole while making the electrode move relative to the workpiece and uses electrodischarge machining to form the straight part; and characterized by further comprising: an inside wall surface machining process which arranges the electrode so that the axis of the electrode becomes vertical to an inside wall surface of the inclined part which is formed by an inclined part machining process while making the electrode move relative to the workpiece to machine the inside wall surface of the inclined part by electrodischarge machining. an inside wall surface machining process which arranges the electrode so that the axis of the electrode becomes vertical to an inside wall surface of the inclined part which is formed by an inclined part machining process while making the electrode move relative to the workpiece to machine the inside wall surface of the inclined part by electrodischarge machining.
Mixture composition of an installation material for a vegetation and substrate layer for natural sports turfs, consisting of a loose mixture of a) organic substances such as topsoil and/or peat, and b) sand having the following characteristics: lava sand is selected as the sand, this being as a 0/3 mm screen fraction broken or unbroken and then rounded off; the percentage of lava sand by volume is approximately 20-45%; c) artificial turf fibres in a length of at least 12 cm to at most 23 cm are added loose to the mixture composition. a) organic substances such as topsoil and/or peat, and b) sand having the following characteristics: lava sand is selected as the sand, this being as a 0/3 mm screen fraction broken or unbroken and then rounded off; the percentage of lava sand by volume is approximately 20-45%; c) artificial turf fibres in a length of at least 12 cm to at most 23 cm are added loose to the mixture composition.
A mobile computing device to display content and to communicate messages of different types, the mobile computing device comprising: a processor; a memory communicatively coupled to the processor, the memory storing a content sharing interface (150 or 202) that when executed by the processor is operative to prompt a user to select a recipient for a message and receive from the user the selection of the recipient for the message, prompt the user to select a transport mechanism for the message and receive from the user the selection of the transport mechanism for the message from a generated list of at least one transport mechanism associated with the selected recipient, format the content of the message according to the selected transport mechanism, the formatting including changing the content from a first format to a second format compatible with the transport mechanism, and display the formatted content in a compose view of the mobile computing device, the compose view including a message composition area operative to edit the formatted content, receive additional message content, and insert a text message string, emoticon, or media object; and a wireless transceiver communicatively coupled to the processor, the wireless transceiver operative to send the formatted content to the selected recipient. a processor; a memory communicatively coupled to the processor, the memory storing a content sharing interface (150 or 202) that when executed by the processor is operative to prompt a user to select a recipient for a message and receive from the user the selection of the recipient for the message, prompt the user to select a transport mechanism for the message and receive from the user the selection of the transport mechanism for the message from a generated list of at least one transport mechanism associated with the selected recipient, format the content of the message according to the selected transport mechanism, the formatting including changing the content from a first format to a second format compatible with the transport mechanism, and display the formatted content in a compose view of the mobile computing device, the compose view including a message composition area operative to edit the formatted content, receive additional message content, and insert a text message string, emoticon, or media object; and a wireless transceiver communicatively coupled to the processor, the wireless transceiver operative to send the formatted content to the selected recipient.
A method for distributive coordination of a plurality of agents to affect at least one target, said method comprising, at a first agent, the steps of: composing a target list, said target list comprising a plurality of targets; ranking targets within said target list; receiving a communication from a second agent that a target has been reserved by the second agent; removing the target that has been reserved by the second agent from said target list; selecting a target for engagement from the target list using target selection criteria and a behaviour-based control system comprising a subsumption architecture, wherein target selection criteria includes a target valuation based on a scoring function comprising a linear combination of a plurality of metrics, and on a prioritization scheme, and wherein the first agent predicts the scores the second agent will obtain for each target, and said first agent chooses a target whose predicted score of said second agent is not the highest of said scores; communicating a reservation of the selected target to at least one other agent; and affecting the reserved target. composing a target list, said target list comprising a plurality of targets; ranking targets within said target list; receiving a communication from a second agent that a target has been reserved by the second agent; removing the target that has been reserved by the second agent from said target list; selecting a target for engagement from the target list using target selection criteria and a behaviour-based control system comprising a subsumption architecture, wherein target selection criteria includes a target valuation based on a scoring function comprising a linear combination of a plurality of metrics, and on a prioritization scheme, and wherein the first agent predicts the scores the second agent will obtain for each target, and said first agent chooses a target whose predicted score of said second agent is not the highest of said scores; communicating a reservation of the selected target to at least one other agent; and affecting the reserved target.
A system for synthesizing microstructures comprising: a light source; a mask having or generating at least one mask feature and being positioned to convey light from the light source; an array of optical elements positioned such that each optical element corresponds to a mask feature projected from the light source via the mask; and, a cylinder having a surface with wells formed therein to align with the optical elements, the surface configured to carry precursor material in which microstructures are formed based on light projected from the light source onto the precursor material. a light source; a mask having or generating at least one mask feature and being positioned to convey light from the light source; an array of optical elements positioned such that each optical element corresponds to a mask feature projected from the light source via the mask; and, a cylinder having a surface with wells formed therein to align with the optical elements, the surface configured to carry precursor material in which microstructures are formed based on light projected from the light source onto the precursor material.
A weapon launch system for a submersible vessel, comprising: - at least one electric actuator capable of performing a series of mechanical actions on a weapon launch tube; - a first box comprising a power module capable of supplying electrical power; - a second box comprising a control module capable of controlling the operation of the electric actuator; - a first security module electrically connecting the power module to the control module; - a second security module electrically connecting the control module to the electric actuator; the boxes (31, 32) being separated from one another and from the electric actuator. - at least one electric actuator capable of performing a series of mechanical actions on a weapon launch tube; - a first box comprising a power module capable of supplying electrical power; - a second box comprising a control module capable of controlling the operation of the electric actuator; - a first security module electrically connecting the power module to the control module; - a second security module electrically connecting the control module to the electric actuator;
Method for determining the fill level of a process medium (4, 4a, 4b) arranged in a container using a fill level meter, wherein the fill level meter is designed as a TDR fill level meter, wherein the fill level meter has at least one signal line for guiding an electromagnetic signal, a signal line head and an electronics unit, wherein the signal line extends into the process medium (4, 4a, 4b) and wherein the method comprises at least the following steps: - generating a pulsed transmitter signal with an amplitude As by the electronics unit, wherein the transmission signal propagates along the signal line as measuring signal with changing amplitude, - detecting and evaluating at least one reflection signal with an amplitude A refl by the electronics unit, wherein the reflection signal is a reflection of the measuring signal on an interface between a first and a second medium, wherein the first medium has a first impedance z 0 and the second medium has a second impedance z 1, wherein the relative permittivity ε r of the second medium is determined at least in dependence on the amplitude of the measuring signal transmitted through the first medium and the amplitude A refl of the reflection signal characterized in that the signal line head is connected to the container via a flange-like process connection, that the second medium is a gaseous medium that is arranged above the process medium (4, 4a, 4b) and that the reflection of the measuring signal takes place on the interface of the preferably flange-like process connection and the interior of the container and that the determination of the relative permittivity ε r, gas of the gaseous medium takes place using the following formula: ε r, gas = z 1 _ gas z 0 SK ⋅ α 0 − A fl A S α 0 + A fl A S 2, wherein A fl is the amplitude of the reflection signal of a reflection on the interface of the preferably flange-like process connection and the interior of the container, wherein As is the amplitude of the generated transmission signal, wherein α 0 characterizes the attenuation of the measuring signal within the signal line head, wherein z 1 _ gas is the impedance of the gaseous phase above the process medium (4, 4a, 4b) and wherein z 0_SK is the impedance of the signal line head. - generating a pulsed transmitter signal with an amplitude As by the electronics unit, wherein the transmission signal propagates along the signal line as measuring signal with changing amplitude, - detecting and evaluating at least one reflection signal with an amplitude A refl by the electronics unit, wherein the reflection signal is a reflection of the measuring signal on an interface between a first and a second medium, wherein the first medium has a first impedance z 0 and the second medium has a second impedance z 1,
A method of monitoring nitric oxide administration to an ex vivo fluid for biological material preservation comprising: administering nitric oxide to an oxygenated ex vivo fluid comprising red blood cells by contacting the ex vivo fluid with a gas comprising a nitric oxide concentration in the range from 0.1 ppm to 300 ppm; monitoring methemoglobin in the ex vivo fluid; adjusting the nitric oxide administration if the methemoglobin levels meet or exceed a threshold of 12% as a percentage of methemoglobin relative to the red blood cells.