A method for the multiplex analysis of multiple analytes comprising the steps: a) Use of a support on which at least two different microparticle populations are immobilized, wherein the different microparticle populations differ in their fluorescence coding and at least two of the differently fluorescence-coded microparticle populations contain microparticles respectively, which are occupied by a particular, specific acceptor molecule population, wherein the acceptor molecule populations of the at least two differently fluorescence-coded microparticle populations differ from one another. b) Measurement of the fluorescence of the support from step a) using an optical resolution 1 prior to bringing the support into contact with the sample to be analyzed or prior to the start of the reaction, said resolution 1: - permitting the differentiation of microparticle singlets, doublets, triplets, multiplets and monolayers, and - allowing the determination of the localized position of the individual immobilized microparticles of the at least two different microparticle populations respectively on the support, taking account of the different fluorescence coding respectively of the at least two different microparticle populations. c) Bringing the support from step a) into contact with the sample to be analyzed, wherein the interaction of the respective analyte with the specific acceptor molecule for it produces a modification in fluorescence on the relevant immobilized microparticle. d) Taking of at least one additional measurement of the fluorescence of the support during or after the contact made or the start of the reaction in step c) with a resolution 2. e) Assignment of the fluorescence values measured with resolution 2 for the individual microparticle singlets, doublets, triplets, multiplets and monolayers which have been locally identified on the support in step b) and assigned to a particular acceptor molecule population. f) Determination of the modification in fluorescence by the contact made in step c) for each locally identified microparticle singlet and each microparticle in a doublet, triplet, multiplet and monolayer on the support. a) Use of a support on which at least two different microparticle populations are immobilized, wherein the different microparticle populations differ in their fluorescence coding and at least two of the differently fluorescence-coded microparticle populations contain microparticles respectively, which are occupied by a particular, specific acceptor molecule population, wherein the acceptor molecule populations of the at least two differently fluorescence-coded microparticle populations differ from one another. b) Measurement of the fluorescence of the support from step a) using an optical resolution 1 prior to bringing the support into contact with the sample to be analyzed or prior to the start of the reaction, said resolution 1: - permitting the differentiation of microparticle singlets, doublets, triplets, multiplets and monolayers, and - allowing the determination of the localized position of the individual immobilized microparticles of the at least two different microparticle populations respectively on the support, taking account of the different fluorescence coding respectively of the at least two different microparticle populations. - permitting the differentiation of microparticle singlets, doublets, triplets, multiplets and monolayers, and - allowing the determination of the localized position of the individual immobilized microparticles of the at least two different microparticle populations respectively on the support, taking account of the different fluorescence coding respectively of the at least two different microparticle populations. c) Bringing the support from step a) into contact with the sample to be analyzed, wherein the interaction of the respective analyte with the specific acceptor molecule for it produces a modification in fluorescence on the relevant immobilized microparticle. d) Taking of at least one additional measurement of the fluorescence of the support during or after the contact made or the start of the reaction in step c) with a resolution 2. e) Assignment of the fluorescence values measured with resolution 2 for the individual microparticle singlets, doublets, triplets, multiplets and monolayers which have been locally identified on the support in step b) and assigned to a particular acceptor molecule population. f) Determination of the modification in fluorescence by the contact made in step c) for each locally identified microparticle singlet and each microparticle in a doublet, triplet, multiplet and monolayer on the support.