An endoscope sheath for use in removing bacterial biofilm from a target site of a human patient, the sheath comprising: an elongated, flexible outer sleeve adapted to receive an insertion tube of an endoscope, the outer sleeve defining a distal end maintaining a viewing window; an irrigation duct formed as an elongated tube having a distal end maintaining a nozzle, the nozzle being secured adjacent the viewing window and oriented to direct a pressurized stream of fluid away from the viewing window and directly against a layer of bacterial biofilm to mechanically remove the bacterial biofilm without substantially damaging an underlying structure of the target site; an aspiration duct formed as an elongated tube having a distal end defining an inlet to remove the fluid as well as biofilm that has been removed from the target site; characterized by: a manifold adapted to be releasably connected to the endoscope and a handle, the manifold comprising: an endoscope connector that is adapted to be releasably secured to a connector flange of the endoscope, and a handle interface, the handle interface comprising: a first coupling head in fluid communication with the aspiration duct, the first coupling head including sealing means for forming a seal with a first receptacle on the handle; a second coupling head in fluid communication with the irrigation duct, the second coupling head including sealing means for forming a seal with a second receptacle in the handle. an elongated, flexible outer sleeve adapted to receive an insertion tube of an endoscope, the outer sleeve defining a distal end maintaining a viewing window; an irrigation duct formed as an elongated tube having a distal end maintaining a nozzle, the nozzle being secured adjacent the viewing window and oriented to direct a pressurized stream of fluid away from the viewing window and directly against a layer of bacterial biofilm to mechanically remove the bacterial biofilm without substantially damaging an underlying structure of the target site; an aspiration duct formed as an elongated tube having a distal end defining an inlet to remove the fluid as well as biofilm that has been removed from the target site; characterized by: a manifold adapted to be releasably connected to the endoscope and a handle, the manifold comprising: an endoscope connector that is adapted to be releasably secured to a connector flange of the endoscope, and a handle interface, the handle interface comprising: a first coupling head in fluid communication with the aspiration duct, the first coupling head including sealing means for forming a seal with a first receptacle on the handle; a second coupling head in fluid communication with the irrigation duct, the second coupling head including sealing means for forming a seal with a second receptacle in the handle. a manifold adapted to be releasably connected to the endoscope and a handle, the manifold comprising: an endoscope connector that is adapted to be releasably secured to a connector flange of the endoscope, and a handle interface, the handle interface comprising: a first coupling head in fluid communication with the aspiration duct, the first coupling head including sealing means for forming a seal with a first receptacle on the handle; a second coupling head in fluid communication with the irrigation duct, the second coupling head including sealing means for forming a seal with a second receptacle in the handle. an endoscope connector that is adapted to be releasably secured to a connector flange of the endoscope, and a handle interface, the handle interface comprising: a first coupling head in fluid communication with the aspiration duct, the first coupling head including sealing means for forming a seal with a first receptacle on the handle; a second coupling head in fluid communication with the irrigation duct, the second coupling head including sealing means for forming a seal with a second receptacle in the handle. a first coupling head in fluid communication with the aspiration duct, the first coupling head including sealing means for forming a seal with a first receptacle on the handle; a second coupling head in fluid communication with the irrigation duct, the second coupling head including sealing means for forming a seal with a second receptacle in the handle.