A method for assaying a presence of a target nucleic acid molecule in a sample, comprising: (a) providing a chip comprising an integrated sensor adjacent to a sample chamber, wherein said sample chamber is configured to retain said sample having said target nucleic acid molecule, and wherein said integrated sensor (i) has a surface including a probe that selectively couples to said target nucleic acid molecule and a control probe that does not couple to said target nucleic acid molecule, wherein said probe and said control probe comprise an energy donor and wherein said target nucleic acid molecule comprises an energy acceptor, and (ii) detects a control signal from said control probe; (b) providing said sample in said sample chamber under conditions that permit said probe to selectively couple to said target nucleic acid molecule; (c) subjecting said surface to a temperature change, wherein said target nucleic acid molecule dissociates from said probe during said temperature change; (d) using said integrated sensor to detect at least one signal from said probe and said control signal from said control probe in real-time while subjecting said surface to said temperature change, wherein said at least one signal increases as said target analyte dissociates from said probe during said temperature change and is generated upon an energy transfer between said energy donor and said energy acceptor; and (e) generating signal versus temperature data using said detected at least one signal, thereby determining said presence of said target nucleic acid molecule in said sample, wherein said signal versus temperature data are calibrated using said control signal. (a) providing a chip comprising an integrated sensor adjacent to a sample chamber, wherein said sample chamber is configured to retain said sample having said target nucleic acid molecule, and wherein said integrated sensor (i) has a surface including a probe that selectively couples to said target nucleic acid molecule and a control probe that does not couple to said target nucleic acid molecule, wherein said probe and said control probe comprise an energy donor and wherein said target nucleic acid molecule comprises an energy acceptor, and (ii) detects a control signal from said control probe; (b) providing said sample in said sample chamber under conditions that permit said probe to selectively couple to said target nucleic acid molecule; (c) subjecting said surface to a temperature change, wherein said target nucleic acid molecule dissociates from said probe during said temperature change; (d) using said integrated sensor to detect at least one signal from said probe and said control signal from said control probe in real-time while subjecting said surface to said temperature change, wherein said at least one signal increases as said target analyte dissociates from said probe during said temperature change and is generated upon an energy transfer between said energy donor and said energy acceptor; and (e) generating signal versus temperature data using said detected at least one signal, thereby determining said presence of said target nucleic acid molecule in said sample, wherein said signal versus temperature data are calibrated using said control signal.