A non-naturally occurring microbial organism having: a methanol metabolic pathway, wherein said non-naturally occurring microbial organism comprises at least one exogenous nucleic acid encoding a MMP enzyme to enhance the availability of reducing equivalents in the presence of methanol, wherein said MMP comprises: (i) (a) a methanol dehydrogenase, a methylenetetrahydrofolate dehydrogenase, a methenyltetrahydrofolate cyclohydrolase and a formyltetrahydrofolate deformylase; (b) an EM9, an EM3, an EM4 and a formyltetrahydrofolate synthetase; (c) an EM9 and a formaldehyde dehydrogenase; (d) an EM9, a S-(hydroxymethyl)glutathione synthase, a glutathione-dependent formaldehyde dehydrogenase and a S-formylglutathione hydrolase; or (e) an EM9, an EM13 and an EM14; (ii) an EM9 and a formaldehyde activating enzyme; or (iii) an EM9, wherein said non-naturally occurring microbial organism further comprises a formaldehyde assimilation pathway, wherein said non-naturally occurring microbial organism comprises at least one exogenous nucleic acid encoding a FAP enzyme to produce an intermediate of glycolysis and/or a metabolic pathway that can be used in the formation of biomass, and wherein said FAP comprises (a) a hexulose-6-phosphate synthase and a 6-phospho-3-hexuloisomerase; (b) a dihydroxyacetone synthase and a dihydroxyacetone kinase; and a 1,4-butanediol pathway, wherein said non-naturally occurring microbial organism comprises at least one exogenous nucleic acid encoding a BDO pathway (BDOP) enzyme to produce BDO, and wherein the methanol dehydrogenase is selected from the group consisting of enzymes represented by GenBankIDs EIJ77596.1, EIJ83020.1, EIJ80770.1, ZP_10132907.1, ZP_10132325.1, ZP_10131932.1, ZP_07048751.1, YP_001699778.1, ZP_10819291.1, YP_004681552.1, NP_00659.2, YP_004758576.1, ZP_09352758.1, ZP_10129817.1, YP_001139613.1, NP_014555.1, WP_007139094.1, NP_343875.1, YP_006863258, NP_394301.1, ZP_10750164.1, YP_023929.1, ZP_08977641.1, ZP_10117398.1, YP_004108045.1, ZP_09753449.1, NP_561852, YP_002138168, YP_001447544, YP_359772, ZP_01220157.1, ZP_07335453.1, YP_001337153, YP_694908, NP_717107, AAC45651, ZP_11313277.1, ZP_16224338.1, YP_001113612, YP_004860127, YP_003310546, YP_001343716, NP_717107, YP_002434746, ZP_10241531.1, YP_005052855, NP_617528, YP_237055, and WP_003897664.1; and wherein the formaldehyde activating enzyme is selected from the group consisting of enzymes represented by GenBank IDs Q9FA38.3, YP_001414948.1 and YP_003755607.1. a methanol metabolic pathway, wherein said non-naturally occurring microbial organism comprises at least one exogenous nucleic acid encoding a MMP enzyme to enhance the availability of reducing equivalents in the presence of methanol, wherein said MMP comprises: (i) (a) a methanol dehydrogenase, a methylenetetrahydrofolate dehydrogenase, a methenyltetrahydrofolate cyclohydrolase and a formyltetrahydrofolate deformylase; (b) an EM9, an EM3, an EM4 and a formyltetrahydrofolate synthetase; (c) an EM9 and a formaldehyde dehydrogenase; (d) an EM9, a S-(hydroxymethyl)glutathione synthase, a glutathione-dependent formaldehyde dehydrogenase and a S-formylglutathione hydrolase; or (e) an EM9, an EM13 and an EM14; (ii) an EM9 and a formaldehyde activating enzyme; or (iii) an EM9, wherein said non-naturally occurring microbial organism further comprises a formaldehyde assimilation pathway, wherein said non-naturally occurring microbial organism comprises at least one exogenous nucleic acid encoding a FAP enzyme to produce an intermediate of glycolysis and/or a metabolic pathway that can be used in the formation of biomass, and wherein said FAP comprises (a) a hexulose-6-phosphate synthase and a 6-phospho-3-hexuloisomerase; (b) a dihydroxyacetone synthase and a dihydroxyacetone kinase; and (i) (a) a methanol dehydrogenase, a methylenetetrahydrofolate dehydrogenase, a methenyltetrahydrofolate cyclohydrolase and a formyltetrahydrofolate deformylase; (b) an EM9, an EM3, an EM4 and a formyltetrahydrofolate synthetase; (c) an EM9 and a formaldehyde dehydrogenase; (d) an EM9, a S-(hydroxymethyl)glutathione synthase, a glutathione-dependent formaldehyde dehydrogenase and a S-formylglutathione hydrolase; or (e) an EM9, an EM13 and an EM14; (a) a methanol dehydrogenase, a methylenetetrahydrofolate dehydrogenase, a methenyltetrahydrofolate cyclohydrolase and a formyltetrahydrofolate deformylase; (b) an EM9, an EM3, an EM4 and a formyltetrahydrofolate synthetase; (c) an EM9 and a formaldehyde dehydrogenase; (d) an EM9, a S-(hydroxymethyl)glutathione synthase, a glutathione-dependent formaldehyde dehydrogenase and a S-formylglutathione hydrolase; or (e) an EM9, an EM13 and an EM14; (ii) an EM9 and a formaldehyde activating enzyme; or (iii) an EM9, wherein said non-naturally occurring microbial organism further comprises a formaldehyde assimilation pathway, wherein said non-naturally occurring microbial organism comprises at least one exogenous nucleic acid encoding a FAP enzyme to produce an intermediate of glycolysis and/or a metabolic pathway that can be used in the formation of biomass, and wherein said FAP comprises (a) a hexulose-6-phosphate synthase and a 6-phospho-3-hexuloisomerase; (b) a dihydroxyacetone synthase and a dihydroxyacetone kinase; and (a) a hexulose-6-phosphate synthase and a 6-phospho-3-hexuloisomerase; (b) a dihydroxyacetone synthase and a dihydroxyacetone kinase; and a 1,4-butanediol pathway, wherein said non-naturally occurring microbial organism comprises at least one exogenous nucleic acid encoding a BDO pathway (BDOP) enzyme to produce BDO, and