A positioning tag operation method used in a positioning tag comprised in a positioning system, which operates according to positioning periods, wherein the positioning tag operation method comprises: setting the positioning tag in an initializing state, selecting an available positioning time interval within each of the positioning periods as a selected positioning time interval and broadcasting a connection request signal, ER, in the selected positioning time interval; receiving a time synchronization signal, CSF, from at least one base station (110A-110F) of the positioning system by the positioning tag; determining that the time synchronization signal, CSF, comprises information of an assigned positioning time interval within each of the positioning periods and the assigned positioning time interval corresponds to the positioning tag by the positioning tag, setting the positioning tag in a positioning state according to the assigned positioning time interval and stop receiving the time synchronization signal, CSF, and broadcasting a positioning signal, PS, in the assigned positioning time interval within each of the positioning periods by the positioning tag in the positioning state, and stopping broadcasting the positioning signal, PS, in the time other than the assigned positioning time interval within the positioning period, setting the positioning tag in the initializing state when a transmission number of the positioning signal exceeds a number threshold or when a time that the positioning tag is in the positioning state exceeds a time threshold, setting the positioning tag in the positioning state when the positioning tag determines that the time synchronization signal comprises device identification information of the positioning tag and the assigned positioning time interval corresponding to the device identification information, wherein the assigned positioning time interval is the same as the selected positioning time interval and selecting the assigned positioning time interval as the selected positioning time interval to broadcast the connection request signal again according to the selected positioning time interval by the positioning tag when the assigned positioning time interval is different from the selected positioning time interval. setting the positioning tag in an initializing state, selecting an available positioning time interval within each of the positioning periods as a selected positioning time interval and broadcasting a connection request signal, ER, in the selected positioning time interval; receiving a time synchronization signal, CSF, from at least one base station (110A-110F) of the positioning system by the positioning tag; determining that the time synchronization signal, CSF, comprises information of an assigned positioning time interval within each of the positioning periods and the assigned positioning time interval corresponds to the positioning tag by the positioning tag, setting the positioning tag in a positioning state according to the assigned positioning time interval and stop receiving the time synchronization signal, CSF, and broadcasting a positioning signal, PS, in the assigned positioning time interval within each of the positioning periods by the positioning tag in the positioning state, and stopping broadcasting the positioning signal, PS, in the time other than the assigned positioning time interval within the positioning period, setting the positioning tag in the initializing state when a transmission number of the positioning signal exceeds a number threshold or when a time that the positioning tag is in the positioning state exceeds a time threshold, setting the positioning tag in the positioning state when the positioning tag determines that the time synchronization signal comprises device identification information of the positioning tag and the assigned positioning time interval corresponding to the device identification information, wherein the assigned positioning time interval is the same as the selected positioning time interval and selecting the assigned positioning time interval as the selected positioning time interval to broadcast the connection request signal again according to the selected positioning time interval by the positioning tag when the assigned positioning time interval is different from the selected positioning time interval.