A method of producing an immune mobilizing monoclonal T-cell receptor against cancer (ImmTAC) comprising a T-cell receptor alpha chain and a TCR beta chain in an E. coli host cell, the method comprising: (a) culturing the E. coli host cell to express the TCR alpha chain and the TCR beta chain of the ImmTAC in a culture medium under conditions comprising: a growth phase comprising a growth temperature and a growth agitation rate, and a production phase comprising a production temperature and a production agitation rate, whereby upon expression the TCR alpha chain and the TCR beta chain fold and assemble to form a biologically active ImmTAC in the E. coli host cell; wherein the E. coli host cell comprises a polynucleotide comprising: a first translational unit encoding the TCR alpha chain of the ImmTAC; a second translational unit encoding the TCR beta chain of the ImmTAC; a third translational unit encoding a peptidyl-prolyl isomerase, wherein the peptidyl-prolyl isomerase is an FkpA protein; and a fourth translational unit encoding a protein disulphide oxidoreductase, wherein the protein disulphide oxidoreductase is a DsbC protein; wherein the growth temperature is in the range of 30°C to 34°C during the growth phase, wherein the production temperature is in the range of 25°C to 29°C during the production phase, wherein the growth agitation rate is sufficient to achieve a peak oxygen uptake rate of the host cell during the growth phase in the range of 3.5 to 4.5 mmol/L/min, and wherein the production agitation rate is sufficient to achieve an oxygen uptake rate of the host cell during the production phase in the range of 1.0 to 3.0 mmol/L/min; and (b) recovering the biologically active ImmTAC from the E. coli host cell. (a) culturing the E. coli host cell to express the TCR alpha chain and the TCR beta chain of the ImmTAC in a culture medium under conditions comprising: a growth phase comprising a growth temperature and a growth agitation rate, and a production phase comprising a production temperature and a production agitation rate, whereby upon expression the TCR alpha chain and the TCR beta chain fold and assemble to form a biologically active ImmTAC in the E. coli host cell; wherein the E. coli host cell comprises a polynucleotide comprising: a first translational unit encoding the TCR alpha chain of the ImmTAC; a second translational unit encoding the TCR beta chain of the ImmTAC; a third translational unit encoding a peptidyl-prolyl isomerase, wherein the peptidyl-prolyl isomerase is an FkpA protein; and a fourth translational unit encoding a protein disulphide oxidoreductase, wherein the protein disulphide oxidoreductase is a DsbC protein; wherein the growth temperature is in the range of 30°C to 34°C during the growth phase, wherein the production temperature is in the range of 25°C to 29°C during the production phase, wherein the growth agitation rate is sufficient to achieve a peak oxygen uptake rate of the host cell during the growth phase in the range of 3.5 to 4.5 mmol/L/min, and wherein the production agitation rate is sufficient to achieve an oxygen uptake rate of the host cell during the production phase in the range of 1.0 to 3.0 mmol/L/min; and a growth phase comprising a growth temperature and a growth agitation rate, and a production phase comprising a production temperature and a production agitation rate, whereby upon expression the TCR alpha chain and the TCR beta chain fold and assemble to form a biologically active ImmTAC in the E. coli host cell; wherein the E. coli host cell comprises a polynucleotide comprising: a first translational unit encoding the TCR alpha chain of the ImmTAC; a second translational unit encoding the TCR beta chain of the ImmTAC; a third translational unit encoding a peptidyl-prolyl isomerase, wherein the peptidyl-prolyl isomerase is an FkpA protein; and a fourth translational unit encoding a protein disulphide oxidoreductase, wherein the protein disulphide oxidoreductase is a DsbC protein; wherein the growth temperature is in the range of 30°C to 34°C during the growth phase, wherein the production temperature is in the range of 25°C to 29°C during the production phase, wherein the growth agitation rate is sufficient to achieve a peak oxygen uptake rate of the host cell during the growth phase in the range of 3.5 to 4.5 mmol/L/min, and wherein the production agitation rate is sufficient to achieve an oxygen uptake rate of the host cell during the production phase in the range of 1.0 to 3.0 mmol/L/min; and a first translational unit encoding the TCR alpha chain of the ImmTAC; a second translational unit encoding the TCR beta chain of the ImmTAC; a third translational unit encoding a peptidyl-prolyl isomerase, wherein the peptidyl-prolyl isomerase is an FkpA protein; and a fourth translational unit encoding a protein disulphide oxidoreductase, wherein the protein disulphide oxidoreductase is a DsbC protein; (b) recovering the biologically active ImmTAC from the E. coli host cell.