A simulated moving-bed type chromatographic separation method comprising separating a weakly adsorptive component, a strongly adsorptive component, and an intermediately adsorptive component that has intermediate adsorptive property in relation to both components, with respect to an adsorbent, with two or more kinds of eluents by using a circulation system in which a plurality of unit packed columns packed with the adsorbent are connected in series and in an endless form via pipes, the weakly adsorptive component, the strongly adsorptive component, and the intermediately adsorptive component being contained in a feed solution, wherein a feed solution supply port F, two or more eluent supply ports D corresponding to the two or more kinds of eluents, an extraction port A of a weakly adsorptive fraction containing the weakly adsorptive component, an extraction port B of an intermediately adsorptive fraction containing the intermediately adsorptive component, and an extraction port C of a strongly adsorptive fraction containing the strongly adsorptive component are provided in the pipes of the circulation system, and positions of the feed solution supply port F, the extraction port A, the extraction port B, and the extraction port C are set as described in the following (a) to (c): (a) the extraction port B is provided on the downstream side of the feed solution supply port F with at least one unit packed column interposed therebetween; (b) the extraction port C is provided in the pipe having the feed solution supply port F or the extraction port C is provided on the upstream side of the feed solution supply port F with at least one unit packed column interposed therebetween; and (c) the extraction port A is provided in the pipe having the extraction port B or the extraction port A is provided on the downstream side of the extraction port B with at least one unit packed column interposed therebetween, and wherein the chromatographic separation method comprises sequentially repeating the following steps and: [step] a step of simultaneously or separately supplying the feed solution and the two or more kinds of eluents from the feed solution supply port F and the two or more eluent supply ports D respectively, and simultaneously or separately extracting the weakly adsorptive fraction, the intermediately adsorptive fraction, and the strongly adsorptive fraction from the extraction port A, the extraction port B, and the extraction port C respectively; and [step] a step of shifting the feed solution supply port F, the eluent supply ports D, the extraction port A, the extraction port B, and the extraction port C to the downstream side, while maintaining a relative positional relationship therebetween, after finishing the step. (a) the extraction port B is provided on the downstream side of the feed solution supply port F with at least one unit packed column interposed therebetween; (b) the extraction port C is provided in the pipe having the feed solution supply port F or the extraction port C is provided on the upstream side of the feed solution supply port F with at least one unit packed column interposed therebetween; and (c) the extraction port A is provided in the pipe having the extraction port B or the extraction port A is provided on the downstream side of the extraction port B with at least one unit packed column interposed therebetween, and wherein the chromatographic separation method comprises sequentially repeating the following steps and: [step] a step of simultaneously or separately supplying the feed solution and the two or more kinds of eluents from the feed solution supply port F and the two or more eluent supply ports D respectively, and simultaneously or separately extracting the weakly adsorptive fraction, the intermediately adsorptive fraction, and the strongly adsorptive fraction from the extraction port A, the extraction port B, and the extraction port C respectively; and [step] a step of shifting the feed solution supply port F, the eluent supply ports D, the extraction port A, the extraction port B, and the extraction port C to the downstream side, while maintaining a relative positional relationship therebetween, after finishing the step. [step] a step of simultaneously or separately supplying the feed solution and the two or more kinds of eluents from the feed solution supply port F and the two or more eluent supply ports D respectively, and simultaneously or separately extracting the weakly adsorptive fraction, the intermediately adsorptive fraction, and the strongly adsorptive fraction from the extraction port A, the extraction port B, and the extraction port C respectively; and [step] a step of shifting the feed solution supply port F, the eluent supply ports D, the extraction port A, the extraction port B, and the extraction port C to the downstream side, while maintaining a relative positional relationship therebetween, after finishing the step.