A method for hybridizing a nucleic acid molecule, comprising i) providing a reaction mixture comprising - at least one first nucleic acid molecule comprising at least partially hybridized against each other - a sense nucleic acid strand having - at least a first nucleotide sequence, and - at least a second nucleotide sequence; - an antisense nucleic acid strand having at least a third nucleotide sequence being at least partially complementary to said a least second nucleotide sequence and being at least partially non-complementary to said at least first nucleotide sequence; - at least one second nucleic acid molecule having a fourth nucleotide sequence being at least partially complementary to said first nucleotide sequence; - hybridization buffer; ii) incubating said reaction mixture under conditions allowing said at least one first nucleic acid molecule to hybridize to said at least one second nucleic acid molecule via a hybridization reaction between said at least first nucleotide sequence and said at least fourth nucleotide sequence, characterized in that said at least one first nucleic acid molecule is provided as follows: - providing said sense nucleic acid strand, - hybridizing at least one primer oligonucleotide to at least a first segment of said second nucleotide sequence, said at least first segment is located upstream and/or downstream of said first nucleotide sequence, - extending said primer oligonucleotide by a nucleotide polymerase to generate said antisense nucleic acid strand, and said least one second nucleic acid molecule is linked to a solid support. i) providing a reaction mixture comprising - at least one first nucleic acid molecule comprising at least partially hybridized against each other - a sense nucleic acid strand having - at least a first nucleotide sequence, and - at least a second nucleotide sequence; - an antisense nucleic acid strand having at least a third nucleotide sequence being at least partially complementary to said a least second nucleotide sequence and being at least partially non-complementary to said at least first nucleotide sequence; - at least one second nucleic acid molecule having a fourth nucleotide sequence being at least partially complementary to said first nucleotide sequence; - hybridization buffer; - at least one first nucleic acid molecule comprising at least partially hybridized against each other - a sense nucleic acid strand having - at least a first nucleotide sequence, and - at least a second nucleotide sequence; - an antisense nucleic acid strand having at least a third nucleotide sequence being at least partially complementary to said a least second nucleotide sequence and being at least partially non-complementary to said at least first nucleotide sequence; - a sense nucleic acid strand having - at least a first nucleotide sequence, and - at least a second nucleotide sequence; - at least a first nucleotide sequence, and - at least a second nucleotide sequence; - an antisense nucleic acid strand having at least a third nucleotide sequence being at least partially complementary to said a least second nucleotide sequence and being at least partially non-complementary to said at least first nucleotide sequence; - at least one second nucleic acid molecule having a fourth nucleotide sequence being at least partially complementary to said first nucleotide sequence; - hybridization buffer; ii) incubating said reaction mixture under conditions allowing said at least one first nucleic acid molecule to hybridize to said at least one second nucleic acid molecule via a hybridization reaction between said at least first nucleotide sequence and said at least fourth nucleotide sequence, characterized in that said at least one first nucleic acid molecule is provided as follows: - providing said sense nucleic acid strand, - hybridizing at least one primer oligonucleotide to at least a first segment of said second nucleotide sequence, said at least first segment is located upstream and/or downstream of said first nucleotide sequence, - extending said primer oligonucleotide by a nucleotide polymerase to generate said antisense nucleic acid strand, and - providing said sense nucleic acid strand, - hybridizing at least one primer oligonucleotide to at least a first segment of said second nucleotide sequence, said at least first segment is located upstream and/or downstream of said first nucleotide sequence, - extending said primer oligonucleotide by a nucleotide polymerase to generate said antisense nucleic acid strand, and