An integrated microfluidic system, including a substrate having a microchannel disposed therein and a membrane attached to a surface of the substrate, wherein the system includes: a two-layer microfluidic device (10, 61), including: only a single, non-elastomeric substrate layer (52, 60) having upper and lower surfaces including a microfluidic channel (62, 64) in one of the upper and lower surfaces, and only a single, non-elastomeric membrane layer (50, 66) selectively attached by a weak solvent bonding agent to the one of the upper and lower surface that includes the microfluidic channel to define unbonded regions where the membrane layer (50, 66) is not attached to the substrate layer (52, 60), wherein the membrane has a relaxed state wherein the membrane lies substantially flat against the upper or lower surface that includes the microfluidic channel and an actuated state wherein the membrane is moved away from the upper or lower surface that includes the microfluidic channel, characterized in that the two-layer microfluidic device further includes: an integrated microfluidic bidirectional pumping structure, including: a plurality of reservoirs; a plurality of inlet/outlet valves each connected to a respective reservoir by one of the microfluidic channels; and a shared drive valve operatively connected to each of the plurality of inlet/outlet valves by a respective one of the microfluidic channels, and in that each reservoir, inlet/outlet valve and shared drive valve being configured to move between an open state where its membrane layer is in an actuated state drawing fluid into a volume below its membrane layer and a subsequent closed state where its membrane layer is in a relaxed state forcing fluid out of the volume. a two-layer microfluidic device (10, 61), including: only a single, non-elastomeric substrate layer (52, 60) having upper and lower surfaces including a microfluidic channel (62, 64) in one of the upper and lower surfaces, and only a single, non-elastomeric membrane layer (50, 66) selectively attached by a weak solvent bonding agent to the one of the upper and lower surface that includes the microfluidic channel to define unbonded regions where the membrane layer (50, 66) is not attached to the substrate layer (52, 60), wherein the membrane has a relaxed state wherein the membrane lies substantially flat against the upper or lower surface that includes the microfluidic channel and an actuated state wherein the membrane is moved only a single, non-elastomeric substrate layer (52, 60) having upper and lower surfaces including a microfluidic channel (62, 64) in one of the upper and lower surfaces, and only a single, non-elastomeric membrane layer (50, 66) selectively attached by a weak solvent bonding agent to the one of the upper and lower surface that includes the microfluidic channel to define unbonded regions where the membrane layer (50, 66) is not attached to the substrate layer (52, 60), wherein the membrane has a relaxed state wherein the membrane lies substantially flat against the upper or lower surface that includes the microfluidic channel and an actuated state wherein the membrane is moved away from the upper or lower surface that includes the microfluidic channel, characterized in that the two-layer microfluidic device further includes: an integrated microfluidic bidirectional pumping structure, including: a plurality of reservoirs; a plurality of inlet/outlet valves each connected to a respective reservoir by one of the microfluidic channels; and a shared drive valve operatively connected to each of the plurality of inlet/outlet valves by a respective one of the microfluidic channels, and in that each reservoir, inlet/outlet valve and shared drive valve being configured to move between an open state where its membrane layer is in an actuated state drawing fluid into a volume below its membrane layer and a subsequent closed state where its membrane layer is in a relaxed state forcing fluid out of the volume. an integrated microfluidic bidirectional pumping structure, including: a plurality of reservoirs; a plurality of inlet/outlet valves each connected to a respective reservoir by one of the microfluidic channels; and a shared drive valve operatively connected to each of the plurality of inlet/outlet valves by a respective one of the microfluidic channels, and in that each reservoir, inlet/outlet valve and shared drive valve being configured to move between an open state where its membrane layer is in an actuated state drawing fluid into a volume below its membrane layer and a subsequent closed state where its membrane layer is in a relaxed state forcing fluid out of the volume. a plurality of reservoirs; a plurality of inlet/outlet valves each connected to a respective reservoir by one of the microfluidic channels; and a shared drive valve operatively connected to each of the plurality of inlet/outlet valves by a respective one of the microfluidic channels, and in that each reservoir, inlet/outlet valve and shared drive valve being configured to move between an open state where its membrane layer is in an actuated state drawing fluid into a volume below its membrane layer and a subsequent closed state where its membrane layer is in a relaxed state forcing fluid out of the volume.