A device (2, 102, 202, 302, 402, 502, 602, 702) for separation of a biological sample (10, 12, 110, 210, 310, 410, 510, 610, 710) into a first phase (14, 114, 214, 314, 414, 514, 614, 714) and a second phase (16, 116, 216, 316, 416, 516, 616, 716), said device comprising: a container (4, 104, 205, 352) including an inlet (6, 106, 206) and an outlet (8, 108, 208), said inlet (6, 106, 206) configured for receiving the biological sample (10, 12, 110, 210, 310, 410, 510, 610, 710); a separator (30, 130, 230, 330, 430, 530, 630, 730) located within said container (4, 104, 205, 352) configured for separating the biological sample (10, 12, 110, 210, 310, 410, 510, 610, 710) into the first phase (14, 114, 214, 314, 414, 514, 614, 714) and the second phase (16, 116, 216, 316, 416, 516, 616, 716), said separator (30, 130, 230, 330, 430, 530, 630, 730) comprising a series of filters (32, 132) of variable pore sizes or multiple grades of fibrous filters (32, 132) configured to progressively filter out different components of the biological sample (10, 12, 110, 210, 310, 410, 510, 610, 710) to yield the first phase (14, 114, 214, 314, 414, 514, 614, 714); a member configured for creating a pressure gradient (40, 140, 204) across the separator (30, 130, 230, 330, 430, 530, 630, 730) to increase a rate of movement of the biological sample (10, 12, 110, 210, 310, 410, 510, 610, 710) through the separator (30, 130, 230, 330, 430, 530, 630, 730) such that the first phase (14, 114, 214, 314, 414, 514, 614, 714) exits the separator (30, 130, 230, 330, 430, 530, 630, 730) prior to the second phase (16, 116, 216, 316, 416, 516, 616, 716); and wherein the container is configured such that the biological sample (10, 12, 110, 210, 310, 410, 510, 610, 710) moves through said container (4, 104, 205, 352) in a vertical direction and the separator (30, 130, 230, 330, 430, 530, 630, 730) includes an open cell foam positioned adjacent the inlet (6, 106,206), the device (2, 102, 202, 302, 402, 502, 602, 702) further including a cell filter located after the separator (30, 130, 230, 330, 430, 530, 630, 730), said cell filter configured to block the second phase (16, 116, 216, 316, 416, 516, 616, 716) from movement therethrough and exiting through the outlet (8, 108, 208), and at least one fibrous filter comprising a chaotic fibrous structure configured to slow particles located within the second phase (16, 116, 216, 316, 416, 516, 616, 716) to further slow the flow of the second phase (16, 116, 216, 316, 416, 516, 616, 716) and increase a flow of the first phase (14, 114, 214, 314, 414, 514, 614, 714) through the separator. a container (4, 104, 205, 352) including an inlet (6, 106, 206) and an outlet (8, 108, 208), said inlet (6, 106, 206) configured for receiving the biological sample (10, 12, 110, 210, 310, 410, 510, 610, 710); a separator (30, 130, 230, 330, 430, 530, 630, 730) located within said container (4, 104, 205, 352) configured for separating the biological sample (10, 12, 110, 210, 310, 410, 510, 610, 710) into the first phase (14, 114, 214, 314, 414, 514, 614, 714) and the second phase (16, 116, 216, 316, 416, 516, 616, 716), said separator (30, 130, 230, 330, 430, 530, 630, 730) comprising a series of filters (32, 132) of variable pore sizes or multiple grades of fibrous filters (32, 132) configured to progressively filter out different components of the biological sample (10, 12, 110, 210, 310, 410, 510, 610, 710) to yield the first phase (14, 114, 214, 314, 414, 514, 614, 714); a member configured for creating a pressure gradient (40, 140, 204) across the separator (30, 130, 230, 330, 430, 530, 630, 730) to increase a rate of movement of the biological sample (10, 12, 110, 210, 310, 410, 510, 610, 710) through the separator (30, 130, 230, 330, 430, 530, 630, 730) such that the first phase (14, 114, 214, 314, 414, 514, 614, 714) exits the separator (30, 130, 230, 330, 430, 530, 630, 730) prior to the second phase (16, 116, 216, 316, 416, 516, 616, 716); and wherein the container is configured such that the biological sample (10, 12, 110, 210, 310, 410, 510, 610, 710) moves through said container (4, 104, 205, 352) in a vertical direction and the separator (30, 130, 230, 330, 430, 530, 630, 730) includes an open cell foam positioned adjacent the inlet (6, 106,206), the device (2, 102, 202, 302, 402, 502, 602, 702) further including a cell filter located after the separator (30, 130, 230, 330, 430, 530, 630, 730), said cell filter configured to block the second phase (16, 116, 216, 316, 416, 516, 616, 716) from movement therethrough and exiting through the outlet (8, 108, 208), and at least one fibrous filter comprising a chaotic fibrous structure configured to slow particles located within the second phase (16, 116, 216, 316, 416, 516, 616, 716) to further slow the flow of the second phase (16, 116, 216, 316, 416, 516, 616, 716) and increase a flow of the first phase (14, 114, 214, 314, 414, 514, 614, 714) through the separator.