A computer implemented method for evaluation of an interaction between an analyte in a fluid sample and a ligand immobilized on a sensor surface of a biosensor during a predetermined acquisition cycle, wherein the predetermined acquisition cycle comprises at least one association phase wherein the sensor surface is put into contact with a fluid sample comprising analyte at a predetermined concentration, the method comprising the steps of: providing, using a graphical interface, a reference binding curve, representing a reference analyte ligand binding interaction for the predetermined acquisition cycle; acquiring, using the biosensor, a sample binding curve for the analyte ligand binding interaction for the predetermined acquisition cycle using the predetermined concentration of said analyte; providing, using a graphical interface, an upper and a lower reference threshold curve defined with respect to the reference binding curve, wherein at least one of the upper and lower threshold curves is movable with respect to the reference binding curve to adjust a threshold range for one or more phases in the predetermined acquisition cycle to set deviation criteria for analysis of the sample binding curve; excluding, using a graphical interface, a section of the sample binding curve exhibiting a disturbance, wherein identification of the disturbance to is based on known analyte ligand interaction and predetermined acquisition conditions, wherein the exclusion contains a transition between an association phase and a dissociation phase of the predetermined acquisition cycle; registering, using a computer, the deviation of the sample binding curve from the reference binding curve, wherein excluded sections of the sample binding curve are not included during the registering of the deviation; classifying, using a computer, the analyte ligand interaction as equivalent to the reference interaction when the registered deviation is less than a predetermined deviation criteria, wherein the predetermined deviation criteria are weighted in response to phases of the predetermined acquisition cycle and the upper and lower reference threshold binding curves; and evaluating, based upon the classification, the molecular binding interaction at a sensing surface independent of interaction models, wherein the binding interaction excludes binding curve sections with disturbances providing, using a graphical interface, a reference binding curve, representing a reference analyte ligand binding interaction for the predetermined acquisition cycle; acquiring, using the biosensor, a sample binding curve for the analyte ligand binding interaction for the predetermined acquisition cycle using the predetermined concentration of said analyte; providing, using a graphical interface, an upper and a lower reference threshold curve defined with respect to the reference binding curve, wherein at least one of the upper and lower threshold curves is movable with respect to the reference binding curve to adjust a threshold range for one or more phases in the predetermined acquisition cycle to set deviation criteria for analysis of the sample binding curve; excluding, using a graphical interface, a section of the sample binding curve exhibiting a disturbance, wherein identification of the disturbance to is based on known analyte ligand interaction and predetermined acquisition conditions, wherein the exclusion contains a transition between an association phase and a dissociation phase of the predetermined acquisition cycle; registering, using a computer, the deviation of the sample binding curve from the reference binding curve, wherein excluded sections of the sample binding curve are not included during the registering of the deviation; classifying, using a computer, the analyte ligand interaction as equivalent to the reference interaction when the registered deviation is less than a predetermined deviation criteria, wherein the predetermined deviation criteria are weighted in response to phases of the predetermined acquisition cycle and the upper and lower reference threshold binding curves; and evaluating, based upon the classification, the molecular binding interaction at a sensing surface independent of interaction models, wherein the binding interaction excludes binding curve sections with disturbances