A method for manufacturing an anti-reflective coating film, comprising the steps of: providing an anti-reflective coating composition comprising 5 to 30 parts by weight of silica nanoparticles having a number average diameter of 5 to 50 nm, 1 to 30 parts by weight of hollow silica particles having a number average diameter of 20 to 70 nm, 5 to 25 parts by weight of a polymerization initiator, and 100 to 500 parts by weight of at least one solvent selected from the group consisting of methyl ethyl ketone, ethyl acetate and acetylacetone, based on 100 parts by weight of a binder; wherein the binder comprises a first (meth)acrylate-based compound having a molecular weight of less than 600, 5 to 30 parts by weight of a second (meth)acrylate-based compound having a molecular weight of 600 to 100000 and 10 to 16 parts by weight of a fluorine-based (meth)acrylate compound substituted with one or more fluorine atoms, based on 100 parts by weight of the first (meth)acrylate-based compound having a molecular weight of less than 600; applying the coating composition onto at least one surface of a substrate, wherein the substrate is generated from polyester-based resins, polycarbonate-based resins, acrylic-based resins, or acetate cellulose resins; drying the coating composition applied to allow at least a part of the first (meth)acrylate-based compound and inorganic nanoparticles to infiltrate into a part of the substrate dissolved by the solvent; and curing the composition to form a first layer that corresponds to the infiltrated region of the substrate, and a second layer comprising hollow particles, which covers the first layer. providing an anti-reflective coating composition comprising 5 to 30 parts by weight of silica nanoparticles having a number average diameter of 5 to 50 nm, 1 to 30 parts by weight of hollow silica particles having a number average diameter of 20 to 70 nm, 5 to 25 parts by weight of a polymerization initiator, and 100 to 500 parts by weight of at least one solvent selected from the group consisting of methyl ethyl ketone, ethyl acetate and acetylacetone, based on 100 parts by weight of a binder; wherein the binder comprises a first (meth)acrylate-based compound having a molecular weight of less than 600, 5 to 30 parts by weight of a second (meth)acrylate-based compound having a molecular weight of 600 to 100000 and 10 to 16 parts by weight of a fluorine-based (meth)acrylate compound substituted with one or more fluorine atoms, based on 100 parts by weight of the first (meth)acrylate-based compound having a molecular weight of less than 600; applying the coating composition onto at least one surface of a substrate, wherein the substrate is generated from polyester-based resins, polycarbonate-based resins, acrylic-based resins, or acetate cellulose resins; drying the coating composition applied to allow at least a part of the first (meth)acrylate-based compound and inorganic nanoparticles to infiltrate into a part of the substrate dissolved by the solvent; and curing the composition to form a first layer that corresponds to the infiltrated region of the substrate, and a second layer comprising hollow particles, which covers the first layer.