A measurement device (10A, 10A', 10B, 10C) comprising: a plurality of optical frequency comb light sources which include at least a first optical frequency comb light source configured to emit first discrete spectral light in which a frequency interval of the adjacent spectra is a first adjacent frequency interval and a second optical frequency comb light source configured to emit second different discrete spectral light in which a frequency interval of the adjacent spectra is a second different adjacent frequency interval; a dispersing part configured to disperse the plurality of spectral lights emitted from the plurality of light sources in mutually different directions according to each spectrum; a focusing part configured to focus the spectra specific to the light sources dispersed by the dispersing part at a plurality of different focal points on a sample so that parts of both a first irradiation region of dispersed first irradiation light having a frequency component of a first frequency value according to the first light and a second irradiation region of dispersed second irradiation light having a frequency component of a second frequency value according to the second light overlap at each position of the plurality of different focal points on the sample a spatial filtering part configured to focus fluorescence beats including information of the sample on which light is focused by the focusing part and which emits light by interference light beats in each of a plurality of overlapping portions where the focal points on the sample overlap at positions conjugate to the overlapping portions and perform spatial filtering on the fluorescence beats; and a detecting part configured to acquire a signal of the fluorescence beats including information of the sample on which light is focused by the focusing part, which light has been emitted from the plurality of overlapping portions due to interference light beats and on which the spatial filtering has been performed by the spatial filtering part. a plurality of optical frequency comb light sources which include at least a first optical frequency comb light source configured to emit first discrete spectral light in which a frequency interval of the adjacent spectra is a first adjacent frequency interval and a second optical frequency comb light source configured to emit second different discrete spectral light in which a frequency interval of the adjacent spectra is a second different adjacent frequency interval; a dispersing part configured to disperse the plurality of spectral lights emitted from the plurality of light sources in mutually different directions according to each spectrum; a focusing part configured to focus the spectra specific to the light sources dispersed by the dispersing part at a plurality of different focal points on a sample so that parts of both a first irradiation region of dispersed first irradiation light having a frequency component of a first frequency value according to the first light and a second irradiation region of dispersed second irradiation light having a frequency component of a second frequency value according to the second light overlap at each position of the plurality of different focal points on the sample a spatial filtering part configured to focus fluorescence beats including information of the sample on which light is focused by the focusing part and which emits light by interference light beats in each of a plurality of overlapping portions where the focal points on the sample overlap at positions conjugate to the overlapping portions and perform spatial filtering on the fluorescence beats; and a detecting part configured to acquire a signal of the fluorescence beats including information of the sample on which light is focused by the focusing part, which light has been emitted from the plurality of overlapping portions due to interference light beats and on which the spatial filtering has been performed by the spatial filtering part.