A system for decontaminating an enclosure defining a region, said system including: a decontamination unit for generating a vaporized sterilant, said decontamination unit comprising: a housing defining a chamber therein, said housing having an inlet and an outlet fluidly communicating with said chamber; a blower for conveying a carrier gas from said inlet, through said chamber and to said outlet of said housing; and a generator for introducing a vaporized sterilant into said carrier gas conveyed through said housing; and a bulkhead assembly for connecting said decontamination unit to said enclosure, said bulkhead assembly comprising: a barrier assembly for sealing an opening of said enclosure, said barrier assembly including: a membrane resistant to said vaporized sterilant, said membrane traversing said opening of said enclosure, and a first port and a second port for allowing said carrier gas to flow through the membrane, a frame assembly for positioning said barrier assembly in said opening of said enclosure; an outlet conduit connected at one end to said outlet of said decontamination unit and at another end to said first port of said barrier assembly, said outlet conduit defining a flow path for conveying said carrier gas from said decontamination unit to said region of said enclosure; and a return conduit connected at one end to said inlet of said decontamination unit and at another end to said second port of said barrier assembly, said return conduit defining a flow path for conveying said carrier gas from said region to said decontamination unit. a decontamination unit for generating a vaporized sterilant, said decontamination unit comprising: a housing defining a chamber therein, said housing having an inlet and an outlet fluidly communicating with said chamber; a blower for conveying a carrier gas from said inlet, through said chamber and to said outlet of said housing; and a generator for introducing a vaporized sterilant into said carrier gas conveyed through said housing; and a housing defining a chamber therein, said housing having an inlet and an outlet fluidly communicating with said chamber; a blower for conveying a carrier gas from said inlet, through said chamber and to said outlet of said housing; and a generator for introducing a vaporized sterilant into said carrier gas conveyed through said housing; and a bulkhead assembly for connecting said decontamination unit to said enclosure, said bulkhead assembly comprising: a barrier assembly for sealing an opening of said enclosure, said barrier assembly including: a membrane resistant to said vaporized sterilant, said membrane traversing said opening of said enclosure, and a first port and a second port for allowing said carrier gas to flow through the membrane, a frame assembly for positioning said barrier assembly in said opening of said enclosure; an outlet conduit connected at one end to said outlet of said decontamination unit and at another end to said first port of said barrier assembly, said outlet conduit defining a flow path for conveying said carrier gas from said decontamination unit to said region of said enclosure; and a return conduit connected at one end to said inlet of said decontamination unit and at another end to said second port of said barrier assembly, said return conduit defining a flow path for conveying said carrier gas from said region to said decontamination unit. a barrier assembly for sealing an opening of said enclosure, said barrier assembly including: a membrane resistant to said vaporized sterilant, said membrane traversing said opening of said enclosure, and a first port and a second port for allowing said carrier gas to flow through the membrane, a membrane resistant to said vaporized sterilant, said membrane traversing said opening of said enclosure, and a first port and a second port for allowing said carrier gas to flow through the membrane, a frame assembly for positioning said barrier assembly in said opening of said enclosure; an outlet conduit connected at one end to said outlet of said decontamination unit and at another end to said first port of said barrier assembly, said outlet conduit defining a flow path for conveying said carrier gas from said decontamination unit to said region of said enclosure; and a return conduit connected at one end to said inlet of said decontamination unit and at another end to said second port of said barrier assembly, said return conduit defining a flow path for conveying said carrier gas from said region to said decontamination unit.