A method of manufacturing a reconstituted tobacco material, comprising: (a) extracting a natural tobacco material with an extracting solvent to obtain an extraction residue and a tobacco extracted liquid containing desired components and undesired components including tobacco-specific nitrosamines; (b) subjecting the tobacco extracted liquid to a fractionating treatment by means of a reverse osmosis membrane to obtain a membrane impermeable fraction containing the desired components and depleted in the undesired components and a membrane permeable fraction depleted in the desired components and enriched in the undesired components; (c) controlling the tobacco extracted liquid during the fractionating treatment to have a temperature suitable for the fractionating treatment; (d) removing precipitates, which are precipitated in the tobacco extracted liquid during the fractionating treatment, from the tobacco extracted liquid; (e) preparing a reconstituted tobacco web containing the extraction residue; and (f) adding the membrane impermeable fraction to the reconstituted tobacco web, wherein the step (b) comprises: (b-1) repeatedly performing the fractionating treatment to obtain a concentrated membrane impermeable fraction, and (b-2) repeating a cycle of consisting of addition of process water to the concentrated membrane impermeable fraction and the fractionating treatment of the membrane impermeable fraction supplied with the process water, wherein the process water is obtained by removing bicarbonate ions from hard water containing bicarbonate ions, wherein the tobacco extracted liquid is controlled to have a temperature within a range of 40 to 80°C in the step (c). (a) extracting a natural tobacco material with an extracting solvent to obtain an extraction residue and a tobacco extracted liquid containing desired components and undesired components including tobacco-specific nitrosamines; (b) subjecting the tobacco extracted liquid to a fractionating treatment by means of a reverse osmosis membrane to obtain a membrane impermeable fraction containing the desired components and depleted in the undesired components and a membrane permeable fraction depleted in the desired components and enriched in the undesired components; (c) controlling the tobacco extracted liquid during the fractionating treatment to have a temperature suitable for the fractionating treatment; (d) removing precipitates, which are precipitated in the tobacco extracted liquid during the fractionating treatment, from the tobacco extracted liquid; (e) preparing a reconstituted tobacco web containing the extraction residue; and (f) adding the membrane impermeable fraction to the reconstituted tobacco web, (b-1) repeatedly performing the fractionating treatment to obtain a concentrated membrane impermeable fraction, and (b-2) repeating a cycle of consisting of addition of process water to the concentrated membrane impermeable fraction and the fractionating treatment of the membrane impermeable fraction supplied with the process water, wherein the process water is obtained by removing bicarbonate ions from hard water containing bicarbonate ions, wherein the tobacco extracted liquid is controlled to have a temperature within a range of 40 to 80°C in the step (c).