A medical imaging device for simultaneously detecting fluorescence and Raman signal for multiple targets, the medical imaging device comprising: alight source configured to emit a laser light; an image guide including an optical fiber bundle configured to guide the laser light emitted from the light source to a test structure and optical signals of an incident light emitted from the test structure or from marker particles bound to the test structure, wherein each of the marker particles comprises a Raman marker material to emit Raman signals receptors, and metallic nanoparticles consisting of at least one of silver (Ag), gold (Au)and copper (Cu), and a fluorescence dye that emits fluorescence signals, or a quantum dot, and each of the marker particles emits fluorescence signals in a first wavelength range and Raman signals in a second wavelength range, and wherein the first and the second wavelength ranges are separated from one another without interference; a light collector connected to the image guide and configured to collect the optical signals including the fluorescence signals and the Raman signals; a scanner connected to the image guide and configured to scan the optical signals including the fluorescence signals and the Raman signals; a light separator comprising a beam splitter connected to the scanner to separate a path of the incident light into a first path including the fluorescence signals in the first wavelength range and a second path including the Raman signals in the second wavelength range; a fluorescence signal detector configured to detect fluorescence signals from the optical signals in the first path separated at the light separator for constructing a fluorescence image indicative of a location of the test structure based on the detected fluorescence signals; and a Raman signal detector configured to detect Raman signals from the optical signals of the second path separated at the light separator for constructing a Raman spectrum of the test structure based on the detected Raman signals. alight source configured to emit a laser light; an image guide including an optical fiber bundle configured to guide the laser light emitted from the light source to a test structure and optical signals of an incident light emitted from the test structure or from marker particles bound to the test structure, wherein each of the marker particles comprises a Raman marker material to emit Raman signals receptors, and metallic nanoparticles consisting of at least one of silver (Ag), gold (Au)and copper (Cu), and a fluorescence dye that emits fluorescence signals, or a quantum dot, and each of the marker particles emits fluorescence signals in a first wavelength range and Raman signals in a second wavelength range, and wherein the first and the second wavelength ranges are separated from one another without interference; a light collector connected to the image guide and configured to collect the optical signals including the fluorescence signals and the Raman signals; a scanner connected to the image guide and configured to scan the optical signals including the fluorescence signals and the Raman signals; a light separator comprising a beam splitter connected to the scanner to separate a path of the incident light into a first path including the fluorescence signals in the first wavelength range and a second path including the Raman signals in the second wavelength range; a fluorescence signal detector configured to detect fluorescence signals from the optical signals in the first path separated at the light separator for constructing a fluorescence image indicative of a location of the test structure based on the detected fluorescence signals; and a Raman signal detector configured to detect Raman signals from the optical signals of the second path separated at the light separator for constructing a Raman spectrum of the test structure based on the detected Raman signals.