A method for detecting a target analyze in a test sample, said method comprising: (a) contacting a test sample and a capture binding ligand that binds to a target analyte, under conditions wherein said capture binding ligand binds said target analyte, if present, in said test sample to form a first complex, said capture binding ligand bound to a first solid support; (b) contacting said first complex with a second binding ligand under conditions wherein said first complex and said second binding ligand bind to form a second complex, wherein said second binding ligand comprises an intermediary enzyme of a peroxide-generating system; (c) isolating said second complex; (d) contacting said second complex with a substrate for said intermediary enzyme of peroxide-generating system under conditions such that products are generated to form a first assay mixture; (e) contacting a peroxide-generating enzyme with a first assay mixture under conditions wherein peroxide is generated to form a peroxide-containing second assay mixture; (f) contacting said peroxide containing second assay mixture with said electroactive moiety comprising a transition metal complex, a self-immolative moiety, and a peroxide sensitive moiety, wherein said SIM joins the PSM to the transition metal complex and wherein said EAM has a first E 0, to form a third assay mixture wherein said peroxide reacts in the solution phase with said PSM of said EAM to release said SIM from said EAM and result in said EAM having a second E 0; (g) contacting said third assay mixture with a second solid support comprising an electrode under conditions such that a covalently attached self-assembled monolayer forms comprising said EAM with said first E 0 and with said second E 0; and (h) detecting for a change between the first E 0 and the second E 0 of said EAM, wherein said change is an indication of the presence of said target analyte. (a) contacting a test sample and a capture binding ligand that binds to a target analyte, under conditions wherein said capture binding ligand binds said target analyte, if present, in said test sample to form a first complex, said capture binding ligand bound to a first solid support; (b) contacting said first complex with a second binding ligand under conditions wherein said first complex and said second binding ligand bind to form a second complex, wherein said second binding ligand comprises an intermediary enzyme of a peroxide-generating system; (c) isolating said second complex; (d) contacting said second complex with a substrate for said intermediary enzyme of peroxide-generating system under conditions such that products are generated to form a first assay mixture; (e) contacting a peroxide-generating enzyme with a first assay mixture under conditions wherein peroxide is generated to form a peroxide-containing second assay mixture; (f) contacting said peroxide containing second assay mixture with said electroactive moiety comprising a transition metal complex, a self-immolative moiety, and a peroxide sensitive moiety, wherein said SIM joins the PSM to the transition metal complex and wherein said EAM has a first E 0, to form a third assay mixture wherein said peroxide reacts in the solution phase with said PSM of said EAM to release said SIM from said EAM and result in said EAM having a second E 0; (g) contacting said third assay mixture with a second solid support comprising an electrode under conditions such that a covalently attached self-assembled monolayer forms comprising said EAM with said first E 0 and with said second E 0; and (h) detecting for a change between the first E 0 and the second E 0 of said EAM, wherein said change is an indication of the presence of said target analyte.