A vessel for containing a fluid sample under conditions which limit evaporation comprising: a vessel body having a body exterior surface and at least one body interior surface, said at least one body interior surface having one or more walls defining a first chamber, a second chamber and a conduit means, wherein said first chamber has a first chamber volume and said second chamber has a second chamber volume, the second chamber volume being significantly less than the first chamber volume, said first chamber having a first chamber opening for receiving at least one of the group selected from a sample and a sample dispensing device, said first chamber opening defining the border of said body exterior surface and said body interior surface, said conduit means being in communication with said first chamber and said second chamber for receiving at least one of the group consisting of sample from said first chamber, a sample from a sample dispensing device and a sample dispensing device passing through or into said conduit means for placing said sample in said conduit means or into said second chamber, said second chamber being in communication with said conduit means for receiving and containing said sample from at least one of the group selected from said sample dispensing device and conduit means, wherein said conduit means being configured such that said sample in said second chamber is substantially isolated from the atmosphere to limit sample evaporation in said second chamber as said sample is contained therein, characterized in that said conduit means further comprises a membrane having a membrane opening having a conduit cross sectional area, said second chamber is constructed and arranged to receive a fluid sample having at least one volume having a fluid level, said fluid level in said second chamber defining a second chamber cross sectional area, said conduit cross sectional area is less than or equal to said second chamber cross sectional area to limit the exchange of atmosphere between said second chamber and said first chamber, and said membrane opening is a slit. a vessel body having a body exterior surface and at least one body interior surface, said at least one body interior surface having one or more walls defining a first chamber, a second chamber and a conduit means, wherein said first chamber has a first chamber volume and said second chamber has a second chamber volume, the second chamber volume being significantly less than the first chamber volume, said first chamber having a first chamber opening for receiving at least one of the group selected from a sample and a sample dispensing device, said first chamber opening defining the border of said body exterior surface and said body interior surface, said conduit means being in communication with said first chamber and said second chamber for receiving at least one of the group consisting of sample from said first chamber, a sample from a sample dispensing device and a sample dispensing device passing through or into said conduit means for placing said sample in said conduit means or into said second chamber, said second chamber being in communication with said conduit means for receiving and containing said sample from at least one of the group selected from said sample dispensing device and conduit means, wherein said conduit means being configured such that said sample in said second chamber is substantially isolated from the atmosphere to limit sample evaporation in said second chamber as said sample is contained therein, characterized in that said conduit means further comprises a membrane having a membrane opening having a conduit cross sectional area, said second chamber is constructed and arranged to receive a fluid sample having at least one volume having a fluid level, said fluid level in said second chamber defining a second chamber cross sectional area, said conduit cross sectional area is less than or equal to said second chamber cross sectional area to limit the exchange of atmosphere between said second chamber and said first chamber, and said membrane opening is a slit.