An isolator system comprising: a glove box that includes an operation portion including a glove for performing an internal operation, and forms an operation space which is isolated from outside; an intake port for taking a gas into the glove box; an exhaust port connected to an exhaust pipe for exhausting the gas in the glove box; a flow rate regulation mechanism that is configured to regulate an exhaust flow rate of the gas, which is exhausted from the exhaust port to the exhaust pipe, to an exhaust flow rate where a differential pressure which is set in advance can be formed between the operation space and the outside of the glove box by a differential pressure forming portion provided in the intake port, characterized by a partition member which partitions an inside of the glove box into the operation space and a buffer space where the exhaust port is formed, and in which a communication port is formed to communicate the operation space and the buffer space with each other; a buffer side gas supply unit that includes the intake port and is configured to supply a buffer gas to the buffer space; an operation space side gas supply unit that is configured to supply an operation space gas to the operation space; and a filter provided in the communication port, wherein the buffer side gas supply unit is configured such that the buffer gas is atmospheric air and the operation space side gas supply unit is configured such that the operation space gas is an inert gas, and wherein the operation space side gas supply unit is configured such that a flow rate of the operation space gas supplied from the operation space side gas supply unit is set at a flow rate where a pressure loss occurring when the buffer gas passes through a differential pressure forming portion of the buffer side gas supply unit becomes larger than a pressure loss occurring when the operation space gas passes through the filter. a glove box that includes an operation portion including a glove for performing an internal operation, and forms an operation space which is isolated from outside; an intake port for taking a gas into the glove box; an exhaust port connected to an exhaust pipe for exhausting the gas in the glove box; a flow rate regulation mechanism that is configured to regulate an exhaust flow rate of the gas, which is exhausted from the exhaust port to the exhaust pipe, to an exhaust flow rate where a differential pressure which is set in advance can be formed between the operation space and the outside of the glove box by a differential pressure forming portion provided in the intake port, characterized by a partition member which partitions an inside of the glove box into the operation space and a buffer space where the exhaust port is formed, and in which a communication port is formed to communicate the operation space and the buffer space with each other; a buffer side gas supply unit that includes the intake port and is configured to supply a buffer gas to the buffer space; an operation space side gas supply unit that is configured to supply an operation space gas to the operation space; and a filter provided in the communication port, wherein the buffer side gas supply unit is configured such that the buffer gas is atmospheric air and the operation space side gas supply unit is configured such that the operation space gas is an inert gas, and wherein the operation space side gas supply unit is configured such that a flow rate of the operation space gas supplied from the operation space side gas supply unit is set at a flow rate where a pressure loss occurring when the buffer gas passes through a differential pressure forming portion of the buffer side gas supply unit becomes larger than a pressure loss occurring when the operation space gas passes through the filter.