A method for producing pyripyropene A, characterized by culturing a microorganism in which the polynucleotides of or below or recombinant vectors comprising them are introduced with pyripyropene E and isolating pyripyropene A via pyripyropene O: polynucleotides having the nucleotide sequence encoding amino acid sequences comprising SEQ ID NOs: 269, 270 and 275, or amino acid sequences comprising SEQ ID NOs: 269, 270 and 275 having one to forty substituted, deleted, added, or inserted residues and having the same enzymatic activity as SEQ ID NOs: 269, 270 and 275, respectively; polynucleotides having the nucleotide sequences of,, or below: nucleotide sequences comprising (a) to (c) below: (a) a nucleotide sequence from 13266 to 15144 of a nucleotide sequence shown in SEQ ID NO:266, (b) a nucleotide sequence from 16220 to 18018 of a nucleotide sequence shown in SEQ ID NO:266, and (c) a nucleotide sequence from 25824 to 27178 of a nucleotide sequence shown in SEQ ID NO:266; nucleotide sequences which are capable of hybridizing with the sequences complementary to the nucleotide sequence in under stringent conditions comprising washing with 2 x saline-sodium citrate buffer and 0.5% Sodium dodecyl sulfate at 60°C for 20 minutes, and then washing with 0.2 x SSC and 0.1 % SDS at 60°C for 15 minutes; nucleotide sequences of in which one to four nucleotides are deleted, substituted, inserted or added,; or nucleotide sequences which are at least 90% identical to the nucleotide sequences in. polynucleotides having the nucleotide sequence encoding amino acid sequences comprising SEQ ID NOs: 269, 270 and 275, or amino acid sequences comprising SEQ ID NOs: 269, 270 and 275 having one to forty substituted, deleted, added, or inserted residues and having the same enzymatic activity as SEQ ID NOs: 269, 270 and 275, respectively; polynucleotides having the nucleotide sequences of,, or below: nucleotide sequences comprising (a) to (c) below: (a) a nucleotide sequence from 13266 to 15144 of a nucleotide sequence shown in SEQ ID NO:266, (b) a nucleotide sequence from 16220 to 18018 of a nucleotide sequence shown in SEQ ID NO:266, and (c) a nucleotide sequence from 25824 to 27178 of a nucleotide sequence shown in SEQ ID NO:266; nucleotide sequences which are capable of hybridizing with the sequences complementary to the nucleotide sequence in under stringent conditions comprising washing with 2 x saline-sodium citrate buffer and 0.5% Sodium dodecyl sulfate at 60°C for 20 minutes, and then washing with 0.2 x SSC and 0.1 % SDS at 60°C for 15 minutes; nucleotide sequences of in which one to four nucleotides are deleted, substituted, inserted or added,; or nucleotide sequences which are at least 90% identical to the nucleotide sequences in. nucleotide sequences comprising (a) to (c) below: (a) a nucleotide sequence from 13266 to 15144 of a nucleotide sequence shown in SEQ ID NO:266, (b) a nucleotide sequence from 16220 to 18018 of a nucleotide sequence shown in SEQ ID NO:266, and (c) a nucleotide sequence from 25824 to 27178 of a nucleotide sequence shown in SEQ ID NO:266; (a) a nucleotide sequence from 13266 to 15144 of a nucleotide sequence shown in SEQ ID NO:266, (b) a nucleotide sequence from 16220 to 18018 of a nucleotide sequence shown in SEQ ID NO:266, and (c) a nucleotide sequence from 25824 to 27178 of a nucleotide sequence shown in SEQ ID NO:266; nucleotide sequences which are capable of hybridizing with the sequences complementary to the nucleotide sequence in under stringent conditions comprising washing with 2 x saline-sodium citrate buffer and 0.5% Sodium dodecyl sulfate at 60°C for 20 minutes, and then washing with 0.2 x SSC and 0.1 % SDS at 60°C for 15 minutes; nucleotide sequences of in which one to four nucleotides are deleted, substituted, inserted or added,; or nucleotide sequences which are at least 90% identical to the nucleotide sequences in.