A method for manufacturing a biochip having fluorescent signal sensing properties, the method comprising: forming a light sensor layer (430, 1030, 1130, 1230) including a plurality of light sensing units (431, 1031, 1131, 1231) on a semiconductor substrate; planarizing a surface of the light sensor layer (430, 1030, 1130, 1230); forming a light filter layer (420, 1020, 1120, 1220) on the planarized light sensor layer (430, 1030, 1130, 1230) for blocking undesirable light from being incident on the light sensor layer (430, 1030, 1130, 1230); and forming a bio-layer (410, 1010, 1110, 1210) on an upper surface of the light filter layer (420, 1020, 1120, 1220); wherein the forming of the bio-layer (410, 1010, 1110, 1210) comprises: forming a metal wiring layer (413, 1013, 1113, 1213) for light blocking and wiring; forming light emitting devices (412, 1012, 1112, 1212) arranged vertically over the metal wiring layer (413, 1013, 1113, 1213) and arranged vertically over corresponding light sensing units, so that light emitted from each of the light emitting devices (412, 1012, 1112, 1212) is blocked from being directly incident on the corresponding light sensing units (431, 1031, 1131, 1231); and forming dam-shaped structures (414, 1014, 1114, 1214) such that a plurality of reaction regions (411, 1011, 1111, 1211) are formed between the dam-shaped structures (414, 1014, 1114, 1214), each of the reaction regions (411, 1011, 1111, 1211) being adapted for a biochemical reaction in a reference sample and a target sample to take place, the dam-shaped structures (414, 1014, 1114, 1214) being formed over the respective light emitting devices (412, 1012, 1112, 1212) or on the upper surface of the light filter layer (420, 1020, 1120, 1220). forming a light sensor layer (430, 1030, 1130, 1230) including a plurality of light sensing units (431, 1031, 1131, 1231) on a semiconductor substrate; planarizing a surface of the light sensor layer (430, 1030, 1130, 1230); forming a light filter layer (420, 1020, 1120, 1220) on the planarized light sensor layer (430, 1030, 1130, 1230) for blocking undesirable light from being incident on the light sensor layer (430, 1030, 1130, 1230); and forming a bio-layer (410, 1010, 1110, 1210) on an upper surface of the light filter layer (420, 1020, 1120, 1220); wherein the forming of the bio-layer (410, 1010, 1110, 1210) comprises: forming a metal wiring layer (413, 1013, 1113, 1213) for light blocking and wiring; forming light emitting devices (412, 1012, 1112, 1212) arranged vertically over the metal wiring layer (413, 1013, 1113, 1213) and arranged vertically over corresponding light sensing units, so that light emitted from each of the light emitting devices (412, 1012, 1112, 1212) is blocked from being directly incident on the corresponding light sensing units (431, 1031, 1131, 1231); and forming dam-shaped structures (414, 1014, 1114, 1214) such that a plurality of reaction regions (411, 1011, 1111, 1211) are formed between the dam-shaped structures (414, 1014, 1114, 1214), each of the reaction regions (411, 1011, 1111, 1211) being adapted for a biochemical reaction in a reference sample and a target sample to take place, the dam-shaped structures (414, 1014, 1114, 1214) being formed over the respective light emitting devices (412, 1012, 1112, 1212) or on the upper surface of the light filter layer (420, 1020, 1120, 1220). forming a metal wiring layer (413, 1013, 1113, 1213) for light blocking and wiring; forming light emitting devices (412, 1012, 1112, 1212) arranged vertically over the metal wiring layer (413, 1013, 1113, 1213) and arranged vertically over corresponding light sensing units, so that light emitted from each of the light emitting devices (412, 1012, 1112, 1212) is blocked from being directly incident on the corresponding light sensing units (431, 1031, 1131, 1231); and forming dam-shaped structures (414, 1014, 1114, 1214) such that a plurality of reaction regions (411, 1011, 1111, 1211) are formed between the dam-shaped structures (414, 1014, 1114, 1214), each of the reaction regions (411, 1011, 1111, 1211) being adapted for a biochemical reaction in a reference sample and a target sample to take place, the dam-shaped structures (414, 1014, 1114, 1214) being formed over the respective light emitting devices (412, 1012, 1112, 1212) or on the upper surface of the light filter layer (420, 1020, 1120, 1220).