mhurhangee/ep-ind-claims huggingface dataset:
A method for forming a released paired tag library comprising a first tag sequence and a second tag sequence that together comprise at least part of a polynucleotide of interest, said method comprising: a) ligating a first adaptor to both ends of a polynucleotide of interest, thereby producing an adaptor modified polynucleotide of interest, (i) wherein the first adaptor and the polynucleotide of interest are double-stranded nucleic acids, (ii) wherein the first adaptor has a sticky and a blunt end and wherein the sticky end of the first adaptor lacks a terminal 5' phosphate residue; and (iii) wherein the polynucleotide of interest has two blunt ends; b) circularizing the adaptor modified polynucleotide of interest by ligating a second adaptor with two sticky ends to the adaptor modified polynucleotide of interest, thereby forming a double-stranded circular nucleic acid molecule having a first nick between the first adaptor and the second adaptor on one strand of the double-stranded circular nucleic molecule and a second nick between the first adaptor and the second adaptor on a different strand of the double-stranded circular nucleic molecule; c) performing a nick translation reaction wherein the first nick and the second nick are translated in opposite directions into the polynucleotide of interest; and d) cleaving the circular nucleic acid molecule at the positions of the translated first and second nicks, thereby forming a released paired tag. a) ligating a first adaptor to both ends of a polynucleotide of interest, thereby producing an adaptor modified polynucleotide of interest, (i) wherein the first adaptor and the polynucleotide of interest are double-stranded nucleic acids, (ii) wherein the first adaptor has a sticky and a blunt end and wherein the sticky end of the first adaptor lacks a terminal 5' phosphate residue; and (iii) wherein the polynucleotide of interest has two blunt ends; (i) wherein the first adaptor and the polynucleotide of interest are double-stranded nucleic acids, (ii) wherein the first adaptor has a sticky and a blunt end and wherein the sticky end of the first adaptor lacks a terminal 5' phosphate residue; and (iii) wherein the polynucleotide of interest has two blunt ends; b) circularizing the adaptor modified polynucleotide of interest by ligating a second adaptor with two sticky ends to the adaptor modified polynucleotide of interest, thereby forming a double-stranded circular nucleic acid molecule having a first nick between the first adaptor and the second adaptor on one strand of the double-stranded circular nucleic molecule and a second nick between the first adaptor and the second adaptor on a different strand of the double-stranded circular nucleic molecule; c) performing a nick translation reaction wherein the first nick and the second nick are translated in opposite directions into the polynucleotide of interest; and d) cleaving the circular nucleic acid molecule at the positions of the translated first and second nicks, thereby forming a released paired tag.
Roll stand with at least two working rolls (6, 6') for rolling strip material and with a hydraulic arrangement for controlling a roll gap of the roll stand, comprising: at least one hydraulic adjusting unit for adjusting the roll gap, the hydraulic adjusting unit comprising a cylinder and an adjusting piston, the adjusting piston dividing the cylinder into a first chamber and a second chamber; a first valve arrangement for variably controlling the adjusting unit; a second valve arrangement (10, 10') for variably controlling the adjusting unit, which is connected in parallel with the first valve arrangement and has a larger nominal volume flow than the first valve arrangement; characterized in that the first valve arrangement is hydraulically connected to the first chamber and the second chamber for double-acting actuation of the adjusting unit, the second valve arrangement is hydraulically connected to the first chamber and the second chamber for double-acting actuation of the adjusting unit, and the hydraulic adjusting unit is capable of being subjected to a working pressure greater than 200 bar from a pressure medium source. at least one hydraulic adjusting unit for adjusting the roll gap, the hydraulic adjusting unit comprising a cylinder and an adjusting piston, the adjusting piston dividing the cylinder into a first chamber and a second chamber; a first valve arrangement for variably controlling the adjusting unit; a second valve arrangement (10, 10') for variably controlling the adjusting unit, which is connected in parallel with the first valve arrangement and has a larger nominal volume flow than the first valve arrangement; characterized in that the first valve arrangement is hydraulically connected to the first chamber and the second chamber for double-acting actuation of the adjusting unit, the second valve arrangement is hydraulically connected to the first chamber and the second chamber for double-acting actuation of the adjusting unit, and the hydraulic adjusting unit is capable of being subjected to a working pressure greater than 200 bar from a pressure medium source.
Assembly of at least two reservoirs (1, 2) of consumable fluids for a motor vehicle, the assembly comprising a first reservoir equipped with a first filling chute and a second reservoir equipped with a second filling chute, each in fluid connection with their respective reservoir (1, 2) and opening into the same filling zone via filling heads (3a, 4a), the second chute being fixed at least temporarily to the first chute in or in the vicinity of the filling zone by a securing part, characterized in that the securing part is W-shaped with two end branches (63, 64) and two internal branches (63a, 64a) respectively associated with an end branch (63, 64), the first chute being housed between a first end branch and a first internal branch adjacent and the second chute being housed between a second end branch and an adjacent second internal branch, the securing part being fixed with the first and second chutes (3, 4) at least one respective securing point (7, 8, 9).
A heart rate detection method carried out in a heart rate detection apparatus, comprising: acquiring (110, 310, 610) a heart rate signal of a user; extracting (120, 320, 620) a heart rate characteristic of time domain from a waveform corresponding to the heart rate signal; judging whether the heart rate characteristic of time domain indicates that a finger of the user completely presses a light source for detecting the heart rate; and outputting (140, 370, 690) a corresponding heart rate value according to the heart rate signal when it is judged that the heart rate characteristic of time domain indicates that the finger of the user completely presses a light source for detecting the heart rate, characterized in that the heart rate characteristic comprises the number of wave ridges and/or wave valleys in the waveform, and/or a wave ridge value of the waveform, which is defined as a peak-to-peak value of the waveform; and the judging whether the heart rate characteristic indicates whether a finger of a user completely presses a light source for detecting the heart rate comprises: judging whether the number of wave ridges and/or wave valleys in the waveform falls within a first predetermined numerical range; and/or judging whether the wave ridge value of the waveform exceeds a predetermined threshold. acquiring (110, 310, 610) a heart rate signal of a user; extracting (120, 320, 620) a heart rate characteristic of time domain from a waveform corresponding to the heart rate signal; judging whether the heart rate characteristic of time domain indicates that a finger of the user completely presses a light source for detecting the heart rate; and outputting (140, 370, 690) a corresponding heart rate value according to the heart rate signal when it is judged that the heart rate characteristic of time domain indicates that the finger of the user completely presses a light source for detecting the heart rate, characterized in that the heart rate characteristic comprises the number of wave ridges and/or wave valleys in the waveform, and/or a wave ridge value of the waveform, which is defined as a peak-to-peak value of the waveform; and the judging whether the heart rate characteristic indicates whether a finger of a user completely presses a light source for detecting the heart rate comprises: judging whether the number of wave ridges and/or wave valleys in the waveform falls within a first predetermined numerical range; and/or judging whether the wave ridge value of the waveform exceeds a predetermined threshold. judging whether the number of wave ridges and/or wave valleys in the waveform falls within a first predetermined numerical range; and/or judging whether the wave ridge value of the waveform exceeds a predetermined threshold.
A method of producing an isolated population of EPO producing cells, said method comprising the steps of: providing differentiated kidney cells comprising peritubular interstitial cells; growing said differentiated kidney cells in vitro, wherein said peritubular interstitial cells are grown in co-culture with other renal cell types; and passaging said differentiated kidney cells, wherein said cells produce EPO after said passaging; thereby producing an isolated population of EPO producing cells. providing differentiated kidney cells comprising peritubular interstitial cells; growing said differentiated kidney cells in vitro, wherein said peritubular interstitial cells are grown in co-culture with other renal cell types; and passaging said differentiated kidney cells, wherein said cells produce EPO after said passaging; thereby producing an isolated population of EPO producing cells.
An aqueous pigment suspension in alkaline pH environment comprising: a) at least one particulate white pigment; b) at least one acrylic acid polymer, including salts thereof, having a weight average molecular weight Mw in the range from 1000 to 12,000 g/mol; c) at least one alkali metal silicate; and in which the aqueous pigment suspension has a pH in the range from 10 to below 12 and a solids content of at least 50 wt.-%, based on the total weight of the aqueous pigment suspension. a) at least one particulate white pigment; b) at least one acrylic acid polymer, including salts thereof, having a weight average molecular weight Mw in the range from 1000 to 12,000 g/mol; c) at least one alkali metal silicate; and
A scroll compressor comprising a fixed scroll and an orbiting scroll, the fixed scroll and the orbiting scroll each including a spiral wrap (21, 31) configured to decrease in height from an outer circumferential side (21B, 31B) to an inner circumferential side (21A, 31A) via a stepped portion (21C, 31C) and an end plate (200, 300) having a stepped wall (20C, 30C) being erected following the stepped portion (21C, 31C) of the counterpart wrap (21, 31), characterised in that an inner circumferential tip seal provided in an inner circumferential side of the stepped portion (21C, 31C) and lying between the wrap (21, 31) and the counterpart end plate (200, 300) and an outer circumferential tip seal provided in an outer circumferential side of the stepped portion (21C, 31C) and lying between the wrap (21, 31) and the counterpart end plate (200, 300) are different in material at least at portions facing the end plate (200, 300); and the step portion (21C, 31C) is positioned in an outer circumferential side of a rotation angle corresponding to a central value of a temperature range within which a temperature of the wrap (21, 31) rises, and within a rotation angle range exceeding 2π in an inner circumferential side from a rotation angle at an outermost circumferential portion of the wrap (21, 31).
A glass-like film, comprising: a hard coat layer and a silica layer, wherein the hard coat layer and the silica layer are formed sequentially, wherein the hard coat layer comprises a cationically cured product of a polyorganosiloxane compound of the following formula F, wherein the silica layer comprises a nitrogen atom and a silica network formed of one or more units selected from the group consisting of units of the following formulae A and B, wherein the silica layer does not contain a linkage (Si-N) of the nitrogen atom and the silicon atom, and wherein a ratio of the silica network in the silica layer is 85 weight% or more and the silica layer comprises the nitrogen atom in an amount of 0.0001 to 6 weight% as measured by X-ray photoelectron spectroscopy: [Formula A] R n SiO (4-n)/2 [Formula B] SiO 3/2 L 1/2 wherein, R is hydrogen, an alkyl group, an alkenyl group, an aryl group, an arylalkyl group, an epoxy group or a (meth)acryloyloxyalkyl group, n is 0 or 1, and L is an alkylene group, [Formula F] (R 1 3 SiO 1/2) a (R 2 2 SiO 2/2) b (R 3 SiO 3/2) c (SiO 4/2) d (RO 1/2) e wherein, R 1 to R 3 are each independently a hydrogen atom, an alkyl group, an alkenyl group, an aryl group or a cation curable functional group, at least one of R 1 to R 3 is a cation curable functional group, R is a hydrogen atom or an alkyl group, and when the sum of a, b, c and d is converted into 1, c/(a + b + c + d) is a number of 0.7 or more, and e/(a + b + c + d) is a number in a range of 0 to 0.4. a hard coat layer and a silica layer, wherein the hard coat layer and the silica layer are formed sequentially, wherein the hard coat layer comprises a cationically cured product of a polyorganosiloxane compound of the following formula F, wherein the silica layer comprises a nitrogen atom and a silica network formed of one or more units selected from the group consisting of units of the following formulae A and B, wherein the silica layer does not contain a linkage (Si-N) of the nitrogen atom and the silicon atom, and wherein a ratio of the silica network in the silica layer is 85 weight% or more and the silica layer comprises the nitrogen atom in an amount of 0.0001 to 6 weight% as measured by X-ray photoelectron spectroscopy: [Formula A] R n SiO (4-n)/2 [Formula B] SiO 3/2 L 1/2 wherein, R is hydrogen, an alkyl group, an alkenyl group, an aryl group, an arylalkyl group, an epoxy group or a (meth)acryloyloxyalkyl group, n is 0 or 1, and L is an alkylene group, [Formula F] (R 1 3 SiO 1/2) a (R 2 2 SiO 2/2) b (R 3 SiO 3/2) c (SiO 4/2) d (RO 1/2) e wherein, R 1 to R 3 are each independently a hydrogen atom, an alkyl group, an alkenyl group, an aryl group or a cation curable functional group, at least one of R 1 to R 3 is a cation curable functional group, R is a hydrogen atom or an alkyl group, and when the sum of a, b, c and d is converted into 1, c/(a + b + c + d) is a number of 0.7 or more, and e/(a + b + c + d) is a number in a range of 0 to 0.4.
A compound of Formula: wherein R is a -CH 2 -R 1 group with R 1 being a substituted or unsubstituted monocyclic aryl or a substituted or unsubstituted monocyclic aromatic heterocycle; or a corresponding salt, solvate, hydrate or oxide thereof.
A mechanical seal arrangement comprising: - a mechanical seal with a rotating slide ring and a stationary slide ring having a sealing gap defined therebetween, - a pretensioning device which pretensions the stationary slide ring in the axial direction (X-X) of the mechanical seal assembly, - a support ring which is arranged on the stationary slide ring, the support ring being axially movable in relation to the stationary slide ring, and - a secondary seal, which is arranged to seal between the support ring and the stationary component, - a fixing device which is arranged for axially movable fixing the support ring to a stationary component, wherein - the pretensioning device is arranged between the support ring and the stationary slide ring, characterized by - a stop being provided at the fixing device which limits a first travel distance by which the axially movable support ring can be moved, - wherein the first travel distance between the support ring and the stop is smaller than a second travel distance between the support ring and the stationary component when pressure reversal of the pressure conditions occurs. - a mechanical seal with a rotating slide ring and a stationary slide ring having a sealing gap defined therebetween, - a pretensioning device which pretensions the stationary slide ring in the axial direction (X-X) of the mechanical seal assembly, - a support ring which is arranged on the stationary slide ring, the support ring being axially movable in relation to the stationary slide ring, and - a secondary seal, which is arranged to seal between the support ring and the stationary component, - a fixing device which is arranged for axially movable fixing the support ring to a stationary component, - the pretensioning device is arranged between the support ring and the stationary slide ring, - a stop being provided at the fixing device which limits a first travel distance by which the axially movable support ring can be moved, - wherein the first travel distance between the support ring and the stop is smaller than a second travel distance between the support ring and the stationary component when pressure reversal of the pressure conditions occurs.
A moulding system comprising a mould cavity containing a moulding material to be moulded under pressure, one or more recesses being provided at the edge of the moulding material to allow the escape of entrapped gas from within the mould cavity during moulding of the moulding material under pressure; wherein the moulding material comprises a preformed thermosetting material comprising a reinforcement part and a bonding part for bonding the reinforcement part to a substrate, the recess being provided at the edge of the bonding part, characterised in that the preform is shaped to provide one or more channels at its edges to provide the one or more recesses to allow the escape of air beyond the extremity of the preform during moulding.
A device for protecting locks, comprising a base which is provided with a seat for a cylinder that can be associated with a lock, and having perimetrically at least two axial seats (16a, 16b, 16c, 16d) for connection with a decorative plate which is provided with at least two fins (29a, 29b, 29c, 29d) which are shaped complementary and can be removably coupled to said at least two seats, characterised in that said at least two fins (29a, 29b, 29c, 29d) and two seats (16a, 16b, 16c, 16d) have, in a plan cross-section, a profile with an irregular shape, said at least two axial seats (16a, 16b, 16c, 16d) being formed on said base and having a length that is shorter than that of said base, each said seat (16a, 16b, 16c, 16d) having, in a transverse cross-section, and therefore in a plan view, a profile with said irregular shape which defines, for each one of said axial seats (16a, 16b, 16c, 16d), a first perimetric edge with a non-linear locus in the sense that it follows a path which, in its locus, produces at least one change of direction.
A cooling device comprising a fresh food compartment wherein foodstuffs are placed to be cooled, a freezing compartment kept at lower temperatures than the fresh food compartment wherein foodstuffs are placed to be frozen, a compressor that compresses and circulates the refrigerant fluid in the refrigeration cycle, a fresh food compartment evaporator that provides the interior of the fresh food compartment to be cooled, a freezing compartment evaporator that provides the interior of the freezing compartment to be cooled, at least one heater disposed on and/or in the vicinity of the fresh food compartment evaporator and a thermostatic switch disposed on and/or in the vicinity of the fresh food compartment evaporator, controlling the operation of the compressor depending on the temperature value detected thereby, characterized by a bimetal switch disposed on and/or in the vicinity of the freezing compartment evaporator, connected in series to the heater and controlling the operation of the heater depending on the temperature value detected thereby.
A mascara applicator (210,230,260,280,310,400) for applying mascara to eyelashes, comprising: a handle (212,232,264,282,312,402); a stem (214,234,266,284,314,406) extending from the handle, defining a longitudinal stem axis and having a first end coupled to the handle and a second end; an applicator head (216,236,262,286,316,408) coupled to the stem second end and supported for movement relative to the handle in a direction substantially parallel to the stem axis, said applicator head including one or more elements projecting from the stem for separating and applying cosmetic to the keratinous fibers; and an actuator operatively coupled to the applicator head for moving the applicator head in at least an axial motion comprising a forward stroke at a forward stroke speed, and a reverse stroke at a reverse stroke speed. a handle (212,232,264,282,312,402); a stem (214,234,266,284,314,406) extending from the handle, defining a longitudinal stem axis and having a first end coupled to the handle and a second end; an applicator head (216,236,262,286,316,408) coupled to the stem second end and supported for movement relative to the handle in a direction substantially parallel to the stem axis, said applicator head including one or more elements projecting from the stem for separating and applying cosmetic to the keratinous fibers; and an actuator operatively coupled to the applicator head for moving the applicator head in at least an axial motion comprising a forward stroke at a forward stroke speed, and a reverse stroke at a reverse stroke speed.
Circuit arrangement (A, B, C) for operating a chain comprising at least one light-emitting diode (13, 13') on an AC voltage, in particular mains voltage, comprising - a rectifier with an input circuit (1, 2) connected upstream of the rectifier for drawing the AC voltage and an output circuit (5-12; 5, 6', 7, 8', 9-12; 45, 46, 6", 8'', 9, 42, 43) connected downstream of the rectifier, into which a rectified AC voltage is output, characterized in that the circuit arrangement (A, B, C) further comprises: - an energy store provided in the output circuit (5-12; 5, 6', 7, 8', 9-12; 45, 46, 6", 8", 9, 42, 43), in particular storage capacitor (5; 45, 46), to which the LED chain can be connected in a parallel circuit, and - a current controller (6, 6', 6"), which is a control element (6, 6', 6"), which is connected in series, in the output circuit (5-12; 5, 6', 7, 8', 9-12; 45, 46, 6", 8", 9, 42, 43), with the parallel circuit formed from the storage capacitor (5; 45, 46) and the LED chain, wherein during operation, it interrupts the charging of the storage capacitor (5; 45, 46) each time a current in the output circuit (5-12; 5, 6', 7, 8', 9-12; 45, 46, 6", 8", 9, 42, 43) has risen to a specific threshold current and enables charging again when the rectified AC voltage in the output circuit (5-12; 5, 6', 7, 8', 9-12; 45, 46, 6'', 8'', 9, 42, 43) has then fallen to a specific threshold voltage, in particular 0 V. - a rectifier with an input circuit (1, 2) connected upstream of the rectifier for drawing the AC voltage and an output circuit (5-12; 5, 6', 7, 8', 9-12; 45, 46, 6", 8'', 9, 42, 43) connected downstream of the rectifier, into which a rectified AC voltage is output, - an energy store provided in the output circuit (5-12; 5, 6', 7, 8', 9-12; 45, 46, 6", 8", 9, 42, 43), in particular storage capacitor (5; 45, 46), to which the LED chain can be connected in a parallel circuit, and - a current controller (6, 6', 6"), which is a control element (6, 6', 6"), which is connected in series, in the output circuit (5-12; 5, 6', 7, 8', 9-12; 45, 46, 6", 8", 9, 42, 43), with the parallel circuit formed from the storage capacitor (5; 45, 46) and the LED chain, wherein during operation, it interrupts the charging of the storage capacitor (5; 45, 46) each time a current in the output circuit (5-12; 5, 6', 7, 8', 9-12; 45, 46, 6", 8", 9, 42, 43) has risen to a specific threshold current and enables charging again when the rectified AC voltage in the output circuit (5-12; 5, 6', 7, 8', 9-12; 45, 46, 6'', 8'', 9, 42, 43) has then fallen to a specific threshold voltage, in particular 0 V.