A computer-implemented method for determining the retention times and concentration values of a plurality of analytes in a mixture in a chromatographic analysis process based on the elution of the analytes of said mixture from a predetermined stationary retention phase to a predetermined eluent mobile phase, comprising: a. acquiring a plurality of data representative of a chromatogram or voltammogram that correlates an absorbance response at at least one excitation radiation having a predetermined wavelength, or a redox potential response, by each analyte of the mixture, in proportion to its quantity in the mobile phase following elution, to the respective retention time by said predetermined stationary phase, the chromatogram or voltammogram comprising a series of peak curves representative of the absorbance or redox potential signals of the respective analytes at different retention times, the area subtended by the peak curve being a function of the concentration of said analytes in the mobile phase following elution; b. determining the retention time of respective analytes through the determination of a plurality of maximum points of said chromatogram or voltammogram, each associated with a respective peak curve, and the distance of the abscissa value of said maximum points with respect to a predetermined origin of said chromatogram or voltammogram; c. determining the inflection points of each peak curve associated with a determined maximum point; d. determining respective Gaussian interpolation curves of each portion of the peak curve comprised between the inflection points of each respective peak curve; e. determining a baseline of said chromatogram or voltammogram; f. determining the points of intersection of each Gaussian interpolation curve and the baseline; g. determining a deconvolution curve of each peak curve by interpolation of the points of the peak curve included between the respective inflection points and of the points of intersection of the respective Gaussian interpolation curve with the baseline; and h. determining a concentration value of each analyte as the difference between the area subtended by the deconvolution curve and the area subtended by the baseline, included between the time values corresponding to the intersection points of the respective Gaussian interpolation curve and the baseline. a. acquiring a plurality of data representative of a chromatogram or voltammogram that correlates an absorbance response at at least one excitation radiation having a predetermined wavelength, or a redox potential response, by each analyte of the mixture, in proportion to its quantity in the mobile phase following elution, to the respective retention time by said predetermined stationary phase, the chromatogram or voltammogram comprising a series of peak curves representative of the absorbance or redox potential signals of the respective analytes at different retention times, the area subtended by the peak curve being a function of the concentration of said analytes in the mobile phase following elution; b. determining the retention time of respective analytes through the determination of a plurality of maximum points of said chromatogram or voltammogram, each associated with a respective peak curve, and the distance of the abscissa value of said maximum points with respect to a predetermined origin of said chromatogram or voltammogram; c. determining the inflection points of each peak curve associated with a determined maximum point; d. determining respective Gaussian interpolation curves of each portion of the peak curve comprised between the inflection points of each respective peak curve; e. determining a baseline of said chromatogram or voltammogram; f. determining the points of intersection of each Gaussian interpolation curve and the baseline; g. determining a deconvolution curve of each peak curve by interpolation of the points of the peak curve included between the respective inflection points and of the points of intersection of the respective Gaussian interpolation curve with the baseline; and h. determining a concentration value of each analyte as the difference between the area subtended by the deconvolution curve and the area subtended by the baseline, included between the time values corresponding to the intersection points of the respective Gaussian interpolation curve and the baseline.