A genome extraction device comprising: an outer chamber partitioned into a plurality of first spaces by an outer chamber partition wall, in which a second protrusion member is formed on a bottom surface of the plurality of first spaces; an inner chamber coupled to the outer chamber through upper openings of the plurality of first spaces and partitioned into a plurality of second spaces by an inner chamber partition wall, wherein, in the inner chamber, upper openings and lower openings of the plurality of second spaces are sealed by a sealing member (S1, S2); a cover configured to cover the upper openings of the plurality of first spaces of the outer chamber and having a first protrusion member for tearing a first sealing member sealing the upper openings of the plurality of second spaces formed on a bottom surface of the cover; and a safety clip detachably coupled to the outer chamber and having a cover support member that allows the first protrusion member and the first sealing member to be spaced apart from each other in a state in which the safety clip is combined with the outer chamber; an outer chamber coupling portion at least partially surrounding a perimeter of an outer surface (100a, 100b) of the outer chamber; and an inner chamber coupling portion extending from the outer chamber coupling portion toward the inner chamber, wherein the inner chamber has a fixing portion coupled to the inner chamber coupling portion at a portion facing the inner chamber coupling portion; wherein, when the inner chamber coupling portion is coupled to the fixing portion, a bottom surface of the inner chamber is located at a position spaced at a predetermined distance from the bottom surface of the outer chamber; and wherein a second sealing member for sealing lower openings of the plurality of second spaces and the second protrusion member are spaced apart from each other in a state in which the inner chamber coupling portion and the fixing portion are combined with each other. an outer chamber partitioned into a plurality of first spaces by an outer chamber partition wall, in which a second protrusion member is formed on a bottom surface of the plurality of first spaces; an inner chamber coupled to the outer chamber through upper openings of the plurality of first spaces and partitioned into a plurality of second spaces by an inner chamber partition wall, wherein, in the inner chamber, upper openings and lower openings of the plurality of second spaces are sealed by a sealing member (S1, S2); a cover configured to cover the upper openings of the plurality of first spaces of the outer chamber and having a first protrusion member for tearing a first sealing member sealing the upper openings of the plurality of second spaces formed on a bottom surface of the cover; and a safety clip detachably coupled to the outer chamber and having a cover support member that allows the first protrusion member and the first sealing member to be spaced apart from each other in a state in which the safety clip is combined with the outer chamber; an outer chamber coupling portion at least partially surrounding a perimeter of an outer surface (100a, 100b) of the outer chamber; and an inner chamber coupling portion extending from the outer chamber coupling portion toward the inner chamber, wherein the inner chamber has a fixing portion coupled to the inner chamber coupling portion at a portion facing the inner chamber coupling portion; wherein, when the inner chamber coupling portion is coupled to the fixing portion, a bottom surface of the inner chamber is located at a position spaced at a predetermined distance from the bottom surface of the outer chamber; and wherein a second sealing member for sealing lower openings of the plurality of second spaces and the second protrusion member are spaced apart from each other in a state in which the inner chamber coupling portion and the fixing portion are combined with each other.