A kit for analysis of one or more of a plurality of Influenza A (Flu A) pathogen-associated target nucleic acid molecule species that may be present in a biological sample, comprising: (a) a first Flu A primer pair for generating a first Flu A amplicon if Flu A is present in the biological sample, the first Flu A primer pair comprising a first Flu A primer and a second Flu A primer, wherein: (i) the first Flu A primer that is substantially complementary to a first Flu A target nucleic acid sequence, is 18 to 100 contiguous bases in length and comprises an oligonucleotide sequence of SEQ ID NO:23; and (ii) the second Flu A primer that is substantially complementary to a second Flu A target nucleic acid sequence, and comprises an oligonucleotide sequence of SEQ ID NO:25; and (b) a second Flu A primer pair for generating a second Flu A amplicon if Flu A is present in the biological sample, the second Flu A primer pair comprising a third Flu A primer and a fourth Flu A primer, wherein: (i) the third Flu A primer that is substantially complementary to a third Flu A target nucleic acid sequence, is 19 to 100 contiguous bases in length, and comprises an oligonucleotide sequence of SEQ ID NO 24; and (ii) the fourth Flu A primer that is substantially complementary to a fourth Flu A target nucleic acid sequence, is 20 to 100 contiguous bases in length, and comprises an oligonucleotide sequence of SEQ ID NOS: 26 and 27; and (c) the probe molecule species is substantially complementary to a first Flu A probe target nucleic acid sequence and comprises an oligonucleotide sequence of SEQ ID NOs: 7 and 12; and (d) the probe molecule species is substantially complementary to a second Flu A probe target nucleic acid sequence and comprises an oligonucleotide sequence of SEQ ID NO: 18. (a) a first Flu A primer pair for generating a first Flu A amplicon if Flu A is present in the biological sample, the first Flu A primer pair comprising a first Flu A primer and a second Flu A primer, wherein: (i) the first Flu A primer that is substantially complementary to a first Flu A target nucleic acid sequence, is 18 to 100 contiguous bases in length and comprises an oligonucleotide sequence of SEQ ID NO:23; and (ii) the second Flu A primer that is substantially complementary to a second Flu A target nucleic acid sequence, and comprises an oligonucleotide sequence of SEQ ID NO:25; and (i) the first Flu A primer that is substantially complementary to a first Flu A target nucleic acid sequence, is 18 to 100 contiguous bases in length and comprises an oligonucleotide sequence of SEQ ID NO:23; and (ii) the second Flu A primer that is substantially complementary to a second Flu A target nucleic acid sequence, and comprises an oligonucleotide sequence of SEQ ID NO:25; and (b) a second Flu A primer pair for generating a second Flu A amplicon if Flu A is present in the biological sample, the second Flu A primer pair comprising a third Flu A primer and a fourth Flu A primer, wherein: (i) the third Flu A primer that is substantially complementary to a third Flu A target nucleic acid sequence, is 19 to 100 contiguous bases in length, and comprises an oligonucleotide sequence of SEQ ID NO 24; and (ii) the fourth Flu A primer that is substantially complementary to a fourth Flu A target nucleic acid sequence, is 20 to 100 contiguous bases in length, and comprises an oligonucleotide sequence of SEQ ID NOS: 26 and 27; and (i) the third Flu A primer that is substantially complementary to a third Flu A target nucleic acid sequence, is 19 to 100 contiguous bases in length, and comprises an oligonucleotide sequence of SEQ ID NO 24; and (ii) the fourth Flu A primer that is substantially complementary to a fourth Flu A target nucleic acid sequence, is 20 to 100 contiguous bases in length, and comprises an oligonucleotide sequence of SEQ ID NOS: 26 and 27; and (c) the probe molecule species is substantially complementary to a first Flu A probe target nucleic acid sequence and comprises an oligonucleotide sequence of SEQ ID NOs: 7 and 12; and (d) the probe molecule species is substantially complementary to a second Flu A probe target nucleic acid sequence and comprises an oligonucleotide sequence of SEQ ID NO: 18.