A method for the detection of at least one target molecule, comprising the following steps: a) providing a mediator probe for the detection of at least one target molecule comprising a probe region and a mediator region, wherein the mediator probe is an oligonucleotide and the probe region is located at the 3' terminus and the mediator region is located at the 5' terminus of the oligonucleotide, wherein there is a chemical, biological and/or physical cleavage site between the regions, and the probe region has affinity for a template molecule and the mediator region has another affinity for a detection molecule, b) providing a detection molecule, wherein the detection molecule is an oligonucleotide and has at least the following regions: • a first region at a 5' terminus of the detection molecule, said first region having a fluorescence acceptor or a fluorescence donor and/or a chemical group for binding to a solid phase and/or a chemical protecting group, • a second region interacting with the mediator region, and • a third region having a fluorescence donor or a fluorescence acceptor and/or a chemical protecting group, c) binding the probe region of the mediator probe to a sequence of the template molecule and/or the target molecule, d) amplifying the template molecule and/or the target molecule by means of a helper molecule, preferably by an amplification process including an isothermal method and/or thermocycling-based method and/or by a PCR, particularly preferably a real-time PCR, e) cleaving the mediator probe at the cleavage site during step d) by the helper molecule from step d) or further helper molecules, and f) binding the cleaved mediator region of the mediator probe to the detection molecule, g) detecting a signal. a) providing a mediator probe for the detection of at least one target molecule comprising a probe region and a mediator region, wherein the mediator probe is an oligonucleotide and the probe region is located at the 3' terminus and the mediator region is located at the 5' terminus of the oligonucleotide, wherein there is a chemical, biological and/or physical cleavage site between the regions, and the probe region has affinity for a template molecule and the mediator region has another affinity for a detection molecule, b) providing a detection molecule, wherein the detection molecule is an oligonucleotide and has at least the following regions: • a first region at a 5' terminus of the detection molecule, said first region having a fluorescence acceptor or a fluorescence donor and/or a chemical group for binding to a solid phase and/or a chemical protecting group, • a second region interacting with the mediator region, and • a third region having a fluorescence donor or a fluorescence acceptor and/or a chemical protecting group, • a first region at a 5' terminus of the detection molecule, said first region having a fluorescence acceptor or a fluorescence donor and/or a chemical group for binding to a solid phase and/or a chemical protecting group, • a second region interacting with the mediator region, and • a third region having a fluorescence donor or a fluorescence acceptor and/or a chemical protecting group, c) binding the probe region of the mediator probe to a sequence of the template molecule and/or the target molecule, d) amplifying the template molecule and/or the target molecule by means of a helper molecule, preferably by an amplification process including an isothermal method and/or thermocycling-based method and/or by a PCR, particularly preferably a real-time PCR, e) cleaving the mediator probe at the cleavage site during step d) by the helper molecule from step d) or further helper molecules, and f) binding the cleaved mediator region of the mediator probe to the detection molecule, g) detecting a signal.