A probe for use as a substrate to an enzyme in a method of measuring enzyme activity by using a multi-dimensional nuclear magnetic resonance method in a diagnosis method of a disease having a correlation with the enzyme activity, wherein the diagnosis method comprises a step of introducing the probe into a living body, and a step of detecting abnormal enzyme activity involved in the disease using said measurement method, in which measurement method a change of multi-dimensional nuclear magnetic resonance signals from nuclear magnetic resonance active nuclei of the probe is measured, and the change is caused by recognition of the probe by the enzyme and a chemical reaction proceeding thereafter, and wherein the nuclear magnetic resonance method at least comprises obtaining said nuclear magnetic resonance signals by using the pulse systems of a two-dimensional nuclear magnetic resonance method and a three or more-dimensional nuclear magnetic resonance method and performing analysis by detecting a presence of the probe based on the nuclear magnetic resonance signal obtained by using the two-dimensional nuclear magnetic resonance method, and by detecting the enzyme activity based on the nuclear magnetic resonance signal obtained by using the three or more-dimensional nuclear magnetic resonance method; and wherein the probe is glucose and is labeled with nuclear magnetic resonance active nuclei by all carbons of the glucose being replaced with 13 C and by 7 hydrogen atoms bound with the carbon of the 1 to 6 positions thereof being all deuterated; or wherein the probe is pyruvic acid or a salt thereof and is labeled with nuclear magnetic resonance active nuclei by carbon at the 2-position and carbon at the 3-position of the pyruvic acid or the salt thereof being replaced with 13 C. a step of introducing the probe into a living body, and a step of detecting abnormal enzyme activity involved in the disease using said measurement method, in which measurement method a change of multi-dimensional nuclear magnetic resonance signals from nuclear magnetic resonance active nuclei of the probe is measured, and the change is caused by recognition of the probe by the enzyme and a chemical reaction proceeding thereafter, and wherein the nuclear magnetic resonance method at least comprises obtaining said nuclear magnetic resonance signals by using the pulse systems of a two-dimensional nuclear magnetic resonance method and a three or more-dimensional nuclear magnetic resonance method and performing analysis by detecting a presence of the probe based on the nuclear magnetic resonance signal obtained by using the two-dimensional nuclear magnetic resonance method, and by detecting the enzyme activity based on the nuclear magnetic resonance signal obtained by using the three or more-dimensional nuclear magnetic resonance method; and wherein the probe is glucose and is labeled with nuclear magnetic resonance active nuclei by all carbons of the glucose being replaced with 13 C and by 7 hydrogen atoms bound with the carbon of the 1 to 6 positions thereof being all deuterated; or wherein the probe is pyruvic acid or a salt thereof and is labeled with nuclear magnetic resonance active nuclei by carbon at the 2-position and carbon at the 3-position of the pyruvic acid or the salt thereof being replaced with 13 C.