Removable cryotransfer system for an electron microscope comprising at least one cartridge comprising at least one recess for holding a sample carrier optionally comprising a flexible element, at least one slit providing with respect to the cartridge parallel loading of the sample carrier, the sample carrier being provided on a support, and a fixing element, preferably a preloaded fixing element, a cartridge block for incorporating the at least one cartridge, a cartridge block transporter comprising a cartridge block mover, and a cartridge block connector, and preferably at least one of an O-ring, a cartridge mover, a pushing aid, a fastener, and a vacuum coupler, a cryo vacuum transfer chamber comprising a tube adapted to receive a microscope sample holder, a valve arranged to receive the cartridge block transporter, and an aligner provided inside tube and arranged for receiving the cartridge block thereon in order to position the cartridge into the tip of a microscope sample holder, a cooling element connected to the aligner in operation in thermal contact with a cooling liquid to keep the aligner at a temperature of < 100K, and optionally at least one of a viewing window, a vacuum pump connector, a further valve, wherein the cooling element preferably comprises a cooling rod, a tube, a vacuum tight connector, and a metal braid, and the microscope sample holder with a tip, a cooling rod, a cooling element in operation adapted to maintain the sample in thermal contact with the cooling rod and the tip to keep the sample at a temperature of < 100K, and optionally at least one of an O-ring (504,504a), a connector (505,506), a foldable protection, a counter grip, a dock, and a passage. at least one cartridge comprising at least one recess for holding a sample carrier optionally comprising a flexible element, at least one slit providing with respect to the cartridge parallel loading of the sample carrier, the sample carrier being provided on a support, and a fixing element, preferably a preloaded fixing element, a cartridge block for incorporating the at least one cartridge, a cartridge block transporter comprising a cartridge block mover, and a cartridge block connector, and preferably at least one of an O-ring, a cartridge mover, a pushing aid, a fastener, and a vacuum coupler, a cryo vacuum transfer chamber comprising a tube adapted to receive a microscope sample holder, a valve arranged to receive the cartridge block transporter, and an aligner provided inside tube and arranged for receiving the cartridge block thereon in order to position the cartridge into the tip of a microscope sample holder, a cooling element connected to the aligner in operation in thermal contact with a cooling liquid to keep the aligner at a temperature of < 100K, and optionally at least one of a viewing window, a vacuum pump connector, a further valve, wherein the cooling element preferably comprises a cooling rod, a tube, a vacuum tight connector, and a metal braid, and the microscope sample holder with a tip, a cooling rod, a cooling element in operation adapted to maintain the sample in thermal contact with the cooling rod and the tip to keep the sample at a temperature of < 100K, and optionally at least one of an O-ring (504,504a), a connector (505,506), a foldable protection, a counter grip, a dock, and a passage.