A method for chromatographically separating analytes of a liquid sample, the method comprising: (i) providing the sample in a first conduit; (ii) providing a first solvent mixture (14a, 14b) for the sample; (iii) causing a flow of the first solvent mixture to pass through a splitting junction so as to be split thereat into a first solvent flow portion that flows along a first pathway (2b, 2c, 2e) that includes the first conduit fluidly connected between a second conduit and a third conduit so as to expel the sample from the first conduit, and a second solvent flow portion that simultaneously flows into and through a second pathway so as to exit said second pathway; (iv) simultaneously providing the expelled sample and the exited first solvent mixture to a mixing tee-junction such that the expelled sample and the exited first solvent mixture mix thereat; (v) providing the mixture of the expelled sample and the exited first solvent mixture to a chromatographic column such that the analytes are transferred to the column so as to become adsorbed to a stationary phase within the chromatographic column; and (vi) causing a flow of a fluid comprising a second solvent mixture (14a, 14b) to pass through the splitting junction so as to be split thereat into a first fluid flow portion that flows along a third pathway (2b, 2e) that includes a fourth conduit fluidly connected between the second conduit and the third conduit, wherein the fourth conduit bypasses the first conduit, and a second fluid flow portion that simultaneously flows into and through the second pathway so as to exit said second pathway, wherein the composition of the fluid comprising the second solvent mixture varies with time and wherein the flow rate of the first fluid flow portion that flows along the third pathway is different from the flow rate of the second fluid flow portion that flows along the second pathway; (vii) combining the first and second fluid flow portions of the fluid comprising the second solvent mixture at the mixing tee-junction; (viii) providing the combined first and second fluid flow portions of the fluid comprising the second solvent mixture to the chromatographic column such that the adsorbed analytes are sequentially desorbed from the stationary phase and expelled from the chromatographic column, wherein the time variation of the composition of the fluid comprising the second solvent mixture is calibrated so as to compensate for the difference in flow rates of the first and second fluid flow portions along the third and second pathways respectively. (i) providing the sample in a first conduit; (ii) providing a first solvent mixture (14a, 14b) for the sample; (iii) causing a flow of the first solvent mixture to pass through a splitting junction so as to be split thereat into a first solvent flow portion that flows along a first pathway (2b, 2c, 2e) that includes the first conduit fluidly connected between a second conduit and a third conduit so as to expel the sample from the first conduit, and a second solvent flow portion that simultaneously flows into and through a second pathway so as to exit said second pathway; (iv) simultaneously providing the expelled sample and the exited first solvent mixture to a mixing tee-junction such that the expelled sample and the exited first solvent mixture mix thereat; (v) providing the mixture of the expelled sample and the exited first solvent mixture to a chromatographic column such that the analytes are transferred to the column so as to become adsorbed to a stationary phase within the chromatographic column; and (vi) causing a flow of a fluid comprising a second solvent mixture (14a, 14b) to pass through the splitting junction so as to be split thereat into a first fluid flow portion that flows along a third pathway (2b, 2e) that includes a fourth conduit fluidly connected between the second conduit and the third conduit, wherein the fourth conduit bypasses the first conduit, and a second fluid flow portion that simultaneously flows into and through the second pathway so as to exit said second pathway, wherein the composition of the fluid comprising the second solvent mixture varies with time and wherein the flow rate of the first fluid flow portion that flows along the third pathway is different from the flow rate of the second fluid flow portion that flows along the second pathway; (vii) combining the first and second fluid flow portions of the fluid comprising the second solvent mixture at the mixing tee-junction; (viii) providing the combined first and second fluid flow portions of the fluid comprising the second solvent mixture to the chromatographic column such that the adsorbed analytes are sequentially desorbed from the stationary phase and expelled from the chromatographic column, wherein the time variation of the composition of the fluid comprising the second solvent mixture is calibrated so as to compensate for the difference in flow rates of the first and second fluid flow portions along the third and second pathways respectively.