A dispensing method which dispenses a first liquid contained in a first vessel which stores the first liquid and a second liquid not miscible with the first liquid and having lower specific gravity than that of the first liquid, and a third liquid not miscible with the second liquid and having higher specific gravity than that of the second liquid and contained in a third vessel which stores the second liquid and the third liquid so as to introduce the first liquid and the third liquid into a second vessel by using a tube one end of which is opened and the other end of which is connected with a reservoir, comprising: a second suction step which sucks the first liquid contained in the first vessel to introduce this first liquid into the tube through the one end of the tube; a third suction step which sucks the second liquid contained in the first vessel to introduce this second liquid into the tube through the one end of the tube; a fourth suction step which sucks the third liquid contained in the third vessel to introduce this third liquid into the tube through the one end of the tube; a fifth suction step which sucks the second liquid contained in the third vessel to introduce this second liquid into the tube through the one end of the tube; and a mixing step which sucks the respective liquids contained in the tube to introduce these liquids into the reservoir such that at least a part of the second liquid sucked in the fifth suction step remains within the tube, mixes the first liquid sucked into the tube in the second suction step and the third liquid sucked into the tube in the fourth suction step to produce a fourth liquid mixed in the reservoir, and mixes the second liquids sucked in the first suction step, the third suction step, and the fifth suction step; characterized in that the method further comprises: a first suction step which sucks the second liquid to introduce this second liquid into the tube through the one end of the tube; a first discharge step which releases the second liquid remaining within the tube to discharge this second liquid into the second vessel; a second discharge step which releases the fourth liquid to discharge this fourth liquid into the second vessel; and a third discharge step which releases at least a part of the second liquid mixed in the mixing step to discharge this second liquid into the second vessel; wherein the second liquid is contained in the second vessel beforehand. a second suction step which sucks the first liquid contained in the first vessel to introduce this first liquid into the tube through the one end of the tube; a third suction step which sucks the second liquid contained in the first vessel to introduce this second liquid into the tube through the one end of the tube; a fourth suction step which sucks the third liquid contained in the third vessel to introduce this third liquid into the tube through the one end of the tube; a fifth suction step which sucks the second liquid contained in the third vessel to introduce this second liquid into the tube through the one end of the tube; and a mixing step which sucks the respective liquids contained in the tube to introduce these liquids into the reservoir such that at least a part of the second liquid sucked in the fifth suction step remains within the tube, mixes the first liquid sucked into the tube in the second suction step and the third liquid sucked into the tube in the fourth suction step to produce a fourth liquid mixed in the reservoir, and mixes the second liquids sucked in the first suction step, the third suction step, and the fifth suction step; characterized in that the method further comprises: a first suction step which sucks the second liquid to introduce this second liquid into the tube through the one end of the tube; a first discharge step which releases the second liquid remaining within the tube to discharge this second liquid into the second vessel; a second discharge step which releases the fourth liquid to discharge this fourth liquid into the second vessel; and a third discharge step which releases at least a part of the second liquid mixed in the mixing step to discharge this second liquid into the second vessel; wherein the second liquid is contained in the second vessel beforehand. a first suction step which sucks the second liquid to introduce this second liquid into the tube through the one end of the tube; a first discharge step which releases the second liquid remaining within the tube to discharge this second liquid into the second vessel; a second discharge step which releases the fourth liquid to discharge this fourth liquid into the second vessel; and a third discharge step which releases at least a part of the second liquid mixed in the mixing step to discharge this second liquid into the second vessel; wherein the second liquid is contained in the second vessel beforehand.