A microfluidic device for independently controlling a plurality of pneumatic microvalves, the microfluidic device being couplable to a pressure source and comprising: i. a first substrate; ii. a flexible membrane covering the first substrate; iii. a second substrate covering the flexible membrane; iv. one or more fluidic channels at least partially defined in the first substrate; v. a pressure line couplable to the pressure source and branching into a plurality of pressure channels, the pressure channels being at least partially defined in the second substrate; vi. the plurality of pneumatic microvalves, each comprising - a portion of one of the fluidic channels, and - a first portion of the pressure channel overlapping the fluidic channel portion, the first pressure channel portion and the fluidic channel portion being separated by the flexible membrane, such that a pressure in the first pressure channel portion can bend the flexible membrane - into the fluidic channel portion and/or out of the first pressure channel portion, thereby closing the fluidic channel portion, or - out of the fluidic channel portion and/or into the first pressure channel portion, thereby opening the fluidic channel portion; and vii. at least one pressure control switch per pressure channel, each comprising - a cavity in the first substrate, - a second portion of the corresponding pressure channel overlapping the cavity, the second pressure channel portion and the cavity being separated by the flexible membrane, and - an actuator for bending the flexible membrane - into the second pressure channel portion and/or out of the cavity, thereby closing the second pressure channel portion, or - out of the second pressure channel portion and/or into the cavity, thereby opening the second pressure channel portion; wherein each actuator can be operated independently. i. a first substrate; ii. a flexible membrane covering the first substrate; iii. a second substrate covering the flexible membrane; iv. one or more fluidic channels at least partially defined in the first substrate; v. a pressure line couplable to the pressure source and branching into a plurality of pressure channels, the pressure channels being at least partially defined in the second substrate; vi. the plurality of pneumatic microvalves, each comprising - a portion of one of the fluidic channels, and - a first portion of the pressure channel overlapping the fluidic channel portion, the first pressure channel portion and the fluidic channel portion being separated by the flexible membrane, such that a pressure in the first pressure channel portion can bend the flexible membrane - into the fluidic channel portion and/or out of the first pressure channel portion, thereby closing the fluidic channel portion, or - out of the fluidic channel portion and/or into the first pressure channel portion, thereby opening the fluidic channel portion; and - a portion of one of the fluidic channels, and - a first portion of the pressure channel overlapping the fluidic channel portion, the first pressure channel portion and the fluidic channel portion being separated by the flexible membrane, - into the fluidic channel portion and/or out of the first pressure channel portion, thereby closing the fluidic channel portion, or - out of the fluidic channel portion and/or into the first pressure channel portion, thereby opening the fluidic channel portion; and vii. at least one pressure control switch per pressure channel, each comprising - a cavity in the first substrate, - a second portion of the corresponding pressure channel overlapping the cavity, the second pressure channel portion and the cavity being separated by the flexible membrane, and - an actuator for bending the flexible membrane - into the second pressure channel portion and/or out of the cavity, thereby closing the second pressure channel portion, or - out of the second pressure channel portion and/or into the cavity, thereby opening the second pressure channel portion; - a cavity in the first substrate, - a second portion of the corresponding pressure channel overlapping the cavity, the second pressure channel portion and the cavity being separated by the flexible membrane, and - an actuator for bending the flexible membrane - into the second pressure channel portion and/or out of the cavity, thereby closing the second pressure channel portion, or - out of the second pressure channel portion and/or into the cavity, thereby opening the second pressure channel portion; - into the second pressure channel portion and/or out of the cavity, thereby closing the second pressure channel portion, or - out of the second pressure channel portion and/or into the cavity, thereby opening the second pressure channel portion;