Method for optimizing automated fluorescence pattern recognition in immunodiagnostics, characterized in that a) prior to taking an image using a camera one or more indicator dyes suitable for identifying relevant tissue or cell structures independently of the type of diagnostic problem are additionally or jointly incubated with a fluorescent dye suitable for visualizing a typical fluorescence pattern produced upon binding of a patient's specific antibodies to solid phase-bound antigens, using a microscope, b) a fluorescence image is taken which renders the fluorescence pattern visible for detecting binding of the patient's specific antibody to solid phase-bound antigens, c) an image is taken of the structure stained by the indicator dye, said structure being identified independently of the fluorescence pattern, d) a calibration image is generated of a microscope slide incubated only with the indicator dye in the absence of any fluorescent dye, with the intensity of the staining being determined as a function of the colour intensity of the indicator dye, e) in the colour channel of the fluorescent dye, the colour intensity of the indicator dye as determined from the calibration image taken is subtracted from the recorded fluorescence patterns, f) the fluorescence images are divided as a function of the structures identified by means of the indicator dye into groups of individual images of similar structures, with quantifiable image features being extracted for said groups, and that g) each of the groups is classified by way of fluorescence pattern allocation by means of classification against the image features of groups of corresponding structures from known fluorescence patterns, wherein the method is used for detecting antibodies in the serum of a patient, and wherein the indicator dye used is propidium iodide. a) prior to taking an image using a camera one or more indicator dyes suitable for identifying relevant tissue or cell structures independently of the type of diagnostic problem are additionally or jointly incubated with a fluorescent dye suitable for visualizing a typical fluorescence pattern produced upon binding of a patient's specific antibodies to solid phase-bound antigens, using a microscope, b) a fluorescence image is taken which renders the fluorescence pattern visible for detecting binding of the patient's specific antibody to solid phase-bound antigens, c) an image is taken of the structure stained by the indicator dye, said structure being identified independently of the fluorescence pattern, d) a calibration image is generated of a microscope slide incubated only with the indicator dye in the absence of any fluorescent dye, with the intensity of the staining being determined as a function of the colour intensity of the indicator dye, e) in the colour channel of the fluorescent dye, the colour intensity of the indicator dye as determined from the calibration image taken is subtracted from the recorded fluorescence patterns, f) the fluorescence images are divided as a function of the structures identified by means of the indicator dye into groups of individual images of similar structures, with quantifiable image features being extracted for said groups, and that g) each of the groups is classified by way of fluorescence pattern allocation by means of classification against the image features of groups of corresponding structures from known fluorescence patterns, wherein the method is used for detecting antibodies in the serum of a patient, and wherein the indicator dye used is propidium iodide.