A micro-fluidic chip, comprising a chip main body, a sample inlet, an air inlet, a liquid driving force inlet, an air subchannel, a main fluid channel and multiple functional chambers provided on the chip main body; the main fluid channel communicates with the multiple functional chambers, the liquid driving force inlet connects to a liquid driving device to facilitate liquid flow in the main fluid channel and the multiple functional chambers; at least one of the multiple functional chambers is a liquid quantification chamber; the liquid quantification chamber has a predetermined volume, and a liquid identification site is provided at a liquid outlet of the liquid quantification chamber, and liquid to be quantified flows into the liquid quantification chamber from a liquid inlet of the liquid quantification chamber and reaches the liquid outlet after filling the liquid quantification chamber; wherein a surface of the liquid quantification chamber is formed by hydrophilic surface modification; a width of the liquid inlet of the liquid quantification chamber is 0.3-5 mm, and a height of the liquid inlet of the liquid quantification chamber is 0.3-3 mm; the liquid outlet of the liquid quantification chamber has a width of 0.3-5 mm and a height of 0.3-3 mm; at least one of the liquid quantification chamber is a sample quantification chamber, and a liquid sample flows into the sample quantification chamber through the sample inlet for quantification; one end of the air subchannel communicates with the air inlet, and the other end communicates with the main fluid channel between the sample quantification chamber and the sample inlet, and a junction point of the other end of the air subchannel and the main fluid channel is 0.5-10 mm away from a liquid inlet of the sample quantification chamber. the main fluid channel communicates with the multiple functional chambers, the liquid driving force inlet connects to a liquid driving device to facilitate liquid flow in the main fluid channel and the multiple functional chambers; at least one of the multiple functional chambers is a liquid quantification chamber; the liquid quantification chamber has a predetermined volume, and a liquid identification site is provided at a liquid outlet of the liquid quantification chamber, and liquid to be quantified flows into the liquid quantification chamber from a liquid inlet of the liquid quantification chamber and reaches the liquid outlet after filling the liquid quantification chamber; wherein a surface of the liquid quantification chamber is formed by hydrophilic surface modification; a width of the liquid inlet of the liquid quantification chamber is 0.3-5 mm, and a height of the liquid inlet of the liquid quantification chamber is 0.3-3 mm; the liquid outlet of the liquid quantification chamber has a width of 0.3-5 mm and a height of 0.3-3 mm; at least one of the liquid quantification chamber is a sample quantification chamber, and a liquid sample flows into the sample quantification chamber through the sample inlet for quantification; one end of the air subchannel communicates with the air inlet, and the other end communicates with the main fluid channel between the sample quantification chamber and the sample inlet, and a junction point of the other end of the air subchannel and the main fluid channel is 0.5-10 mm away from a liquid inlet of the sample quantification chamber.