mhurhangee/ep-ind-claims huggingface dataset:
A method of amplifying a target DNA sequence, said method comprising the steps of: a) providing a reaction mixture comprising (i) an oligonucleotide primer having a cleavage domain positioned 5' of a blocking group, said blocking group linked at or near the end of the 3'-end of the oligonucleotide primer wherein said blocking group inhibits primer extension and/or the primer from serving as a template for DNA synthesis, (ii) a sample nucleic acid that may or may not have the target sequence, (iii) a cleaving enzyme, wherein said cleaving enzyme is a thermostable RNase H2 enzyme that gains activity at the elevated temperatures employed in the reaction and has reduced activity at lower temperatures, and (iv) a polymerase; b) hybridizing the primer to the target DNA sequence to form a double-stranded substrate; c) cleaving the hybridized primer with said cleaving enzyme at a point within or adjacent to the cleavage domain to remove the blocking group from the primer; and d) extending the primer with the polymerase. a) providing a reaction mixture comprising (i) an oligonucleotide primer having a cleavage domain positioned 5' of a blocking group, said blocking group linked at or near the end of the 3'-end of the oligonucleotide primer wherein said blocking group inhibits primer extension and/or the primer from serving as a template for DNA synthesis, (ii) a sample nucleic acid that may or may not have the target sequence, (iii) a cleaving enzyme, wherein said cleaving enzyme is a thermostable RNase H2 enzyme that gains activity at the elevated temperatures employed in the reaction and has reduced activity at lower temperatures, and (iv) a polymerase; (i) an oligonucleotide primer having a cleavage domain positioned 5' of a blocking group, said blocking group linked at or near the end of the 3'-end of the oligonucleotide primer wherein said blocking group inhibits primer extension and/or the primer from serving as a template for DNA synthesis, (ii) a sample nucleic acid that may or may not have the target sequence, (iii) a cleaving enzyme, wherein said cleaving enzyme is a thermostable RNase H2 enzyme that gains activity at the elevated temperatures employed in the reaction and has reduced activity at lower temperatures, and (iv) a polymerase; b) hybridizing the primer to the target DNA sequence to form a double-stranded substrate; c) cleaving the hybridized primer with said cleaving enzyme at a point within or adjacent to the cleavage domain to remove the blocking group from the primer; and d) extending the primer with the polymerase.
Interferometer system, including optical means (2, 3, 4, 5) arranged for directing light along a first interferometer path and second interferometer path, and for combining the light for allowing interferometry, wherein the first interferometer path is provided with a first light transmitting structure having a rotational position that is adjustable with respect to an optical axis of the first path, characterized in that the system includes a removal or displacement means for automatically removing and/or replacing the first light transmitting structure from and/or back into the first interferometer path. wherein the first interferometer path is provided with a first light transmitting structure having a rotational position that is adjustable with respect to an optical axis of the first path, characterized in that the system includes a removal or displacement means for automatically removing and/or replacing the first light transmitting structure from and/or back into the first interferometer path.
A microparticle measurement device comprising: a plurality of first tank units (3a, 3b) to which a liquid is supplied via respective supply channels (51a, 51b) from a second tank unit outside of the microparticle measurement device, and which are respectively connected in parallel; and a valve unit that is connected to the plurality of first tank units (3a, 3b), and which switches to a state in which it is possible to deliver the liquid to a flow channel through which microparticles flow; characterized by a supply pump unit that supplies the liquid from the second tank unit to the plurality of first tank units (3a, 3b); and a control unit configured to drive the valve unit and the supply pump unit in conjunction based on a residual quantity of the liquid in the plurality of first tank units (3a, 3b). a plurality of first tank units (3a, 3b) to which a liquid is supplied via respective supply channels (51a, 51b) from a second tank unit outside of the microparticle measurement device, and which are respectively connected in parallel; and a valve unit that is connected to the plurality of first tank units (3a, 3b), and which switches to a state in which it is possible to deliver the liquid to a flow channel through which microparticles flow; characterized by a supply pump unit that supplies the liquid from the second tank unit to the plurality of first tank units (3a, 3b); and a control unit configured to drive the valve unit and the supply pump unit in conjunction based on a residual quantity of the liquid in the plurality of first tank units (3a, 3b).
A pressing apparatus comprising: a presser including a second member configured to come close to or separate from a first member (12, 15, 101); an elastic member configured to apply force in a direction in which the second member comes close to the first member (12, 15, 101) to the presser, the elastic member being mounted on the presser; an actuator configured to apply force to the presser in a direction which causes the second member to come closer to the first member (12, 15, 101) or in a direction which separates the second member from the first member (12, 15, 101), the actuator being mounted on the presser; an acquirer configured to acquire a parameter containing at least one of a position of the presser, a speed of the presser, and force applied by the actuator to the presser; and a controller configured to feedback-control the actuator based on the parameter. a presser including a second member configured to come close to or separate from a first member (12, 15, 101); an elastic member configured to apply force in a direction in which the second member comes close to the first member (12, 15, 101) to the presser, the elastic member being mounted on the presser; an actuator configured to apply force to the presser in a direction which causes the second member to come closer to the first member (12, 15, 101) or in a direction which separates the second member from the first member (12, 15, 101), the actuator being mounted on the presser; an acquirer configured to acquire a parameter containing at least one of a position of the presser, a speed of the presser, and force applied by the actuator to the presser; and a controller configured to feedback-control the actuator based on the parameter.
Use of at least one compound of the formula Ia or Ib in which R6 stands for a radical selected from - H, - (CR7H-CR8H-O) n CR9H-CR10H-OH, where n=0 to 20, - straight-chain or branched alkyl group having 1 to 20 C atoms, - straight-chain or branched alkenyl or alkynyl group having 2 to 20 C atoms and one or more double or triple bonds, where the alkyl, alkenyl or alkynyl group may also contain one or more cyclohexyl or aryl groups, in which R7, R8, R9 and R10 stand, idependently of one another, for H, methyl or ethyl, as plasticizer. - H, - (CR7H-CR8H-O) n CR9H-CR10H-OH, where n=0 to 20, - straight-chain or branched alkyl group having 1 to 20 C atoms, - straight-chain or branched alkenyl or alkynyl group having 2 to 20 C atoms and one or more double or triple bonds,
A high-purity ferritic stainless steel sheet with excellent oxidation resistance and high-temperature strength, consisting of, % by mass, C: 0.001 to 0.03%, Si: 0.01 to 2%, Mn: 0.01 to 1.5%, P: 0.005 to 0.05%, S: 0.0001 to 0.01%, Cr: 16.0% to 30%, N: 0.001 to 0.03%, A1: 0.05 to 3%, and Sn: 0.01 to 1% and optionally one or more of Nb: 0.5% or less, Ti: 0.5% or less, Ni: 0.5% or less, Cu: 0.5% or less, Mo: 0.5% or less, V: 0.5% or less, Zr: 0.5% or less, Co: 0.5% or less, Mg: 0.005% or less, B: 0.005% or less, and Ca: 0.005% or less and/or optionally one or more of La: 0.1% or less, Y: 0.1% or less, Hf: 0.1% or less, and REM: 0.1% or less, with the remainder being Fe and unavoidable impurities wherein the high-purity ferritic stainless steel sheet has both an oxidation resistance wherein abnormal oxidation does not occur in the continuous oxidation test at 980°C for 200 hr determined in accordance with the method disclosed in the description and a high-temperature strength of 0.2% Proof Stress at 800°C ≥ 35 MPa determined in accordance with the method disclosed in the description and Tensile Strength ≥ 55 MPa determined in accordance with the method disclosed in the description.
A device for continuous heat-treating of foods, comprising a conduit made of a dielectric material in which a product to be treated flows, the conduit having at least a pair of electrodes (110, 161) located in the conduit, which are subjected to a potential difference of at least 2000 Volts with a frequency of at least 25 MHz, and a rotor made of a dielectric material located internally of the conduit, characterised in that the rotor has a core from which a plurality of fins parallel to the rotor axis rises, which fins have a section of any shape, are distributed along a length and over the whole perimeter of the core, so that any section of the rotor orthogonal to the rotor axis comprises only one fin, and are of a height such as to contact an internal surface of the conduit.
A method of measuring a quantity of a substrate, comprising the steps of: providing a biosensor to which sample liquid containing a substrate is supplied; providing a measuring device which includes temperature measuring means for measuring a temperature inside the measuring device; detecting a temperature change between a temperature measured before the quantity of the substrate is measured and a temperature measured when the quantity of the substrate is measured; and determining, according to the temperature change, whether the quantity of the substrate is measured or not. providing a biosensor to which sample liquid containing a substrate is supplied; providing a measuring device which includes temperature measuring means for measuring a temperature inside the measuring device; detecting a temperature change between a temperature measured before the quantity of the substrate is measured and a temperature measured when the quantity of the substrate is measured; and determining, according to the temperature change, whether the quantity of the substrate is measured or not.
A method of expanding B10 cells producing IL-10 ex vivo comprising contacting B cells harvested from a subject with IL-21 and isolating the B10 cells, wherein the B10 cells are isolated by selecting for cell surface expression of at least one of CD1d, CD5, or CD24 and optionally wherein the B10 cells are further isolated based on production of IL-10 or based on expression of cell surface molecules including IgM, IgD, IgG, IgA, IgE, CD19, CD20, CD21, CD22, CD24, CD40, CD72, CD79a, CD79b, CD1d, CD5, CD9, CD10, CD1d, CD23, CD27, CD38, CD48, CD80, CD86, CD138, CD148 or combinations thereof.
A method for acceleration control of a hybrid vehicle comprising at least two separate power supplies including an electric battery powering at least one electric machine of the vehicle and capable of being recharged by an electric machine upon a deceleration phase, characterized in that at a given moment an estimation is carried out (43, 44) of: - the energy (E bat decel) that can be recovered by the battery upon a next deceleration and; - the energy (E bat mesurée) stored in the battery; the sum (E bat future) of these two energies being compared to a predetermined threshold (E bat seuil), the authorisation to deliver an additional electric power to the electric machine from the battery during an acceleration phase being granted if the sum is higher than the threshold, and the energy (E bat decel) that can be recovered is estimated as a function of the speed of the vehicle at the given moment, from characteristics of the vehicle and hypotheses concerning the future deceleration profiles. - the energy (E bat decel) that can be recovered by the battery upon a next deceleration and; - the energy (E bat mesurée) stored in the battery;
Method for electronically processing surroundings information of a motor vehicle, in which method the surroundings information is detected by at least one detection unit of the motor vehicle, wherein personal information included in the surroundings information is anonymised and/or deleted depending on whether the person to whom the personal information relates has made an electronic request, characterised in that the motor vehicle is provided with a perceivable identifier with which the motor vehicle is characterised as a motor vehicle detecting the surroundings using the detection unit, wherein the identifier is dynamically changed during the movement of the motor vehicle, such that an association is established between a point in time at which the motor vehicle is located at a specific location and the point in time at which the identifier has a time-specific identifier appearance as a result of its dynamic change, wherein it is determined depending on the association that a person was located at this point in time at the location of the motor vehicle when the person enters into communication with the evaluation unit using this specific identifier appearance of the identifier, this being determined in particular due to the fact that personal information relating to this person was detected, wherein the person can make contact with an electronic evaluation unit using a communication unit with this identifier so as to find out what possible actions there are in order to learn whether personal information relating to his/her person is included in the surroundings information.
The enantiomeric form of a 6-chromanol derivative, wherein the derivative is (6-hydroxy-2,5,7,8-tetramethylchroman-2-yl)(piperazin-1-yl)methanone and pharmaceutically acceptable salts thereof for use in the prophylaxis or treatment of vasoconstriction related disorders or conditions wherein said vasoconstriction related disorders or conditions is selected from the group consisting of pulmonary hypertension, portal hypertension, vasospastic diseases, Raynaud's disease, acrocyanosis, livedo reticularis, post-traumatic dystrophy, (pre)eclampsia, Buerger's disease, pulmonary arterial hypertension, heart failure with preserved ejection fraction, and cardiac hypertrophy and fibrosis.
A method for encoding a signal, comprising: performing a classification decision process on high frequency signals of input signals, wherein the classification decision process determines a current frame type of the high frequency signals, the frame types of the high frequency signals include a transient signal and a non-transient signal; adaptively encoding the high frequency signals according to the result of the classification decision process; and outputting the encoded bitstream of the low frequency signals, adaptive encoded bitstream of the high frequency signals, and the result of the classification decision process; wherein the step of adaptively encoding the high frequency signals according to the result of the classification decision process further comprises: encoding four time envelopes and four spectral envelopes per frame for the transient signal; and encoding eight spectral envelopes and no time envelope per frame for the non-transient signal. performing a classification decision process on high frequency signals of input signals, wherein the classification decision process determines a current frame type of the high frequency signals, the frame types of the high frequency signals include a transient signal and a non-transient signal; adaptively encoding the high frequency signals according to the result of the classification decision process; and outputting the encoded bitstream of the low frequency signals, adaptive encoded bitstream of the high frequency signals, and the result of the classification decision process; wherein the step of adaptively encoding the high frequency signals according to the result of the classification decision process further comprises: encoding four time envelopes and four spectral envelopes per frame for the transient signal; and encoding eight spectral envelopes and no time envelope per frame for the non-transient signal. encoding four time envelopes and four spectral envelopes per frame for the transient signal; and encoding eight spectral envelopes and no time envelope per frame for the non-transient signal.
A vacuum plasma source having a plasma discharge source including a cathode and an anode, the anode comprising an anode surface, said anode surface being an extended surface with at least one cavity having an opening to a surrounding of said anode surface, characterised in that said anode and said cathode are configured to be electrically supplied by an electric signal comprising a DC component of at least one of a voltage of at least 10 V and of a current of at least 10 A, and in that the smallest cross sectional width W of the cavity is between 3 mm and 100 mm and the depth T of the cavity is of at least half W, and in that the plasma source further comprises adjacent said at least one cavity a magnet arrangement adapted to generate a magnetic field in said at least one cavity, said magnet arrangement being controllable so as to generate said magnetic field controllably variable.
An information processor comprising: a storage configured to store information related to an area (STORE) in which a user moves around; and a position calculator configured to calculate, based on a transmission signal of a communication device that moves together with the user, positions in the area of the communication device with the lapse of time; characterized by a measuring unit configured to measure time during which each of virtual points continuously exists in a region with movement of the user, each of the virtual points being defined at a point of intersection between virtual lines (Lx, Ly) that are arranged in grid in the area (STORE), the region centering at each position (Cm) that is calculated by the position calculator, wherein a size of the region is set to contain at least two of the virtual points. a storage configured to store information related to an area (STORE) in which a user moves around; and a position calculator configured to calculate, based on a transmission signal of a communication device that moves together with the user, positions in the area of the communication device with the lapse of time; characterized by a measuring unit configured to measure time during which each of virtual points continuously exists in a region with movement of the user, each of the virtual points being defined at a point of intersection between virtual lines (Lx, Ly) that are arranged in grid in the area (STORE), the region centering at each position (Cm) that is calculated by the position calculator, wherein a size of the region is set to contain at least two of the virtual points.
Bait box comprising - a bottom plate, - a cover and - at least one curved side wall which is connected at least in part to the bottom plate or the cover, while bottom plate, cover and side wall together form at least one compartment with a filling opening inside the bait box, an entrance opening at the side of the bait box and a passage, the passage being designed in a spiral way between entrance opening and compartment, with a turning of at least 360°. - a bottom plate, - a cover and - at least one curved side wall which is connected at least in part to the bottom plate or the cover,
A catheter system (400, 450) for a catheter pump, comprising: an elongate catheter body having a distal portion including an expandable cannula having an inlet and an outlet, the expandable cannula having a delivery profile and an operational profile larger than the delivery profile; an impeller assembly disposed in the expandable cannula and including an impeller shaft and an impeller body including one or more impeller blades, the impeller blade(s) drawing fluid into the expandable cannula when rotated in the fluid; and a sheath (404, 454) having a cannula retention zone (408, 458) disposed over the expandable cannula and a separation zone (420, 470), the cannula retention zone (408, 458) having a first configuration adapted to retain the expandable cannula in the delivery profile, characterised in that a separation device is disposed on the catheter system (400, 450) proximal of the sheath (404, 454) at least when the cannula retention zone (408, 458) of the sheath (404, 454) is disposed over the expandable cannula, the separation device being adapted to separate the separation zone (420, 470) into a first portion and a second portion disposed across a gap, the gap enabling the elongate catheter body to pass between the first and second portions (472A, 472B) so that the sheath (404, 454) can be removed from the elongate catheter body. an elongate catheter body having a distal portion including an expandable cannula having an inlet and an outlet, the expandable cannula having a delivery profile and an operational profile larger than the delivery profile; an impeller assembly disposed in the expandable cannula and including an impeller shaft and an impeller body including one or more impeller blades, the impeller blade(s) drawing fluid into the expandable cannula when rotated in the fluid; and a sheath (404, 454) having a cannula retention zone (408, 458) disposed over the expandable cannula and a separation zone (420, 470), the cannula retention zone (408, 458) having a first configuration adapted to retain the expandable cannula in the delivery profile, characterised in that a separation device is disposed on the catheter system (400, 450) proximal of the sheath (404, 454) at least when the cannula retention zone (408, 458) of the sheath (404, 454) is disposed over the expandable cannula, the separation device being adapted to separate the separation zone (420, 470) into a first portion and a second portion disposed across a gap, the gap enabling the elongate catheter body to pass between the first and second portions (472A, 472B) so that the sheath (404, 454) can be removed from the elongate catheter body.
A method of making zinc particles comprising centrifugally atomizing molten zinc in an atmosphere of less than about 1% oxygen to provide the zinc particles, wherein at least 30% by weight of the zinc particles are spherical, the spherical zinc particles having a mean particle size between 100 μm and 300 μm, each spherical zinc particle having an aspect ratio of between 1.0 and 2.0, wherein the aspect ratio is equal to the ratio of the longest axis of the zinc particle to the mean perpendicular bisector of the longest axis, the centrifugal atomization comprising contacting the molten zinc with a spinning cup that breaks the molten zinc into droplets that after cooling provide the zinc particles, wherein the cup has a depth between 0.1 cm and 10 cm, and the cup rotates at 6000 rpm to 8500 rpm.
Rotor for a reluctance machine comprising a cylindrical soft-magnetic element, wherein the soft-magnetic element has cutouts for forming magnetic flux barriers (2, 3, 4, 5) and at least some of the cutouts are filled with an electrically conductive and magnetically non-conductive filler material, in order to form a starting cage, wherein the ratio of the entire cross-sectional area of the filled regions of the flux barriers (2, 3, 4, 5) to the entire cross-sectional area of the corresponding regions of the non-filled flux barriers (2, 3, 4) for at least one rotor pole is at least 0.2, characterized in that the ratio between the cross-sectional areas of the filled region and the non-filled region of the flux barrier which is situated on the inside in the radial direction of the rotor is in the range of between 0.35 and 0.6.