A method of analyzing cells to distinguish normal and cancerous colon tissues, the method comprising: measuring a number of cells which are nuclear stained, in a to-be-determined tissue, and acquiring a histogram showing a fluorescence intensity based on a result of the measurement; analyzing the histogram, and acquiring data of predetermined parameters which are elements which function as an index for determining a cancer tissue, and which appear as a characteristic pattern in a DNA histogram; comparing the data of the parameters with first thresholds predetermined for the parameters, the first thresholds being determined on the basis of a Receiver Operating Characteristic analysis on tissue taken from cancer patients with respect to the predetermined parameters, to perform a first cancer determination for each of the parameters on the to-be-determined tissue; performing a scoring process for each of the parameters, on a result of the first cancer determination for each of the parameters, to calculate scores of the parameters; characterized in that it further comprises adding the scores of the parameters with one another, thereby performing a second cancer determination on the to-be-determined tissue and in that the scoring process is a process in which scoring is performed based on information of a result of the first cancer determination for each of the parameters and in which weighting is performed among the parameters based on a degree of contribution to the second cancer determination and characterized in that the parameters include at least two or more of: a ratio of a number of all cells of a normal DNA amount to a number of all cells in the histogram; a ratio of a number of DNA amplified cells to a number of all cells in the histogram; a ratio of a debris number to a number of all cells in the histogram; a width of a peak of a normal distribution of cells of a normal DNA amount in the histogram; an area under a curve of a waveform which is obtained by performing a fast Fourier transform of the histogram; and a number of all cells in the histogram. measuring a number of cells which are nuclear stained, in a to-be-determined tissue, and acquiring a histogram showing a fluorescence intensity based on a result of the measurement; analyzing the histogram, and acquiring data of predetermined parameters which are elements which function as an index for determining a cancer tissue, and which appear as a characteristic pattern in a DNA histogram; comparing the data of the parameters with first thresholds predetermined for the parameters, the first thresholds being determined on the basis of a Receiver Operating Characteristic analysis on tissue taken from cancer patients with respect to the predetermined parameters, to perform a first cancer determination for each of the parameters on the to-be-determined tissue; performing a scoring process for each of the parameters, on a result of the first cancer determination for each of the parameters, to calculate scores of the parameters; characterized in that it further comprises adding the scores of the parameters with one another, thereby performing a second cancer determination on the to-be-determined tissue and in that the scoring process is a process in which scoring is performed based on information of a result of the first cancer determination for each of the parameters and in which weighting is performed among the parameters based on a degree of contribution to the second cancer determination and characterized in that the parameters include at least two or more of: a ratio of a number of all cells of a normal DNA amount to a number of all cells in the histogram; a ratio of a number of DNA amplified cells to a number of all cells in the histogram; a ratio of a debris number to a number of all cells in the histogram; a width of a peak of a normal distribution of cells of a normal DNA amount in the histogram; an area under a curve of a waveform which is obtained by performing a fast Fourier transform of the histogram; and a number of all cells in the histogram.