A method for measuring glucose and cortisol levels in earwax comprising the steps of: Extracting earwax samples from the ear by any suitable means; Preparing samples of earwax for the measurement of cortisol and glucose levels in accordance with the measurement methods; Measuring cortisol and glucose using any known means or methods wherein the levels of cortisol and glucose are interpreted as the average cortisol and glucose levels, wherein: The extraction of earwax is carried out by means of an extraction device comprising: A handle having a first end and a second end, said second end having coupling means; A removable sponge head (tip) comprising a base and a longitudinally extending elongated member directly depending from an upper portion of the base, wherein the lower portion of the base has coupling means for receiving the coupling means of the handle, and wherein the elongated member has a star shaped cross section; An elongated sponge having a centrally located longitudinal housing for receiving the elongated member of the base; Wherein the earwax is obtained by inserting the tip with the sponge in the ear and rotating the sponge inside the ear canal; The preparation of the sample is carried out by: adding a PBS buffer solution to a tube; the sponge is separated from its plastic support and introduced into the tube; after the sponge absorbed the entire solution, the sponge is repeatedly squeezed and absorbed; squeezing the sponge dry and removing it from the tube; drying the resulting solution, preferably by displacement with N 2; resuspending the resulting content in ultrapure water; and storing the resulting solution until use, preferably at 4° C; and The cortisol levels are measured by means of ELISA techniques and the glucose levels are measured by means of using a kit for determining glucose levels from the dissolved ear-wax solution, following manufacturer instructions. Extracting earwax samples from the ear by any suitable means; Preparing samples of earwax for the measurement of cortisol and glucose levels in accordance with the measurement methods; Measuring cortisol and glucose using any known means or methods wherein the levels of cortisol and glucose are interpreted as the average cortisol and glucose levels, The extraction of earwax is carried out by means of an extraction device comprising: A handle having a first end and a second end, said second end having coupling means; A removable sponge head (tip) comprising a base and a longitudinally extending elongated member directly depending from an upper portion of the base, wherein the lower portion of the base has coupling means for receiving the coupling means of the handle, and wherein the elongated member has a star shaped cross section; An elongated sponge having a centrally located longitudinal housing for receiving the elongated member of the base; Wherein the earwax is obtained by inserting the tip with the sponge in the ear and rotating the sponge inside the ear canal; A handle having a first end and a second end, said second end having coupling means; A removable sponge head (tip) comprising a base and a longitudinally extending elongated member directly depending from an upper portion of the base, wherein the lower portion of the base has coupling means for receiving the coupling means of the handle, and wherein the elongated member has a star shaped cross section; An elongated sponge having a centrally located longitudinal housing for receiving the elongated member of the base; Wherein the earwax is obtained by inserting the tip with the sponge in the ear and rotating the sponge inside the ear canal; The preparation of the sample is carried out by: adding a PBS buffer solution to a tube; the sponge is separated from its plastic support and introduced into the tube; after the sponge absorbed the entire solution, the sponge is repeatedly squeezed and absorbed; squeezing the sponge dry and removing it from the tube; drying the resulting solution, preferably by displacement with N 2; resuspending the resulting content in ultrapure water; and storing the resulting solution until use, preferably at 4° C; and The cortisol levels are measured by means of ELISA techniques and the glucose levels are measured by means of using a kit for determining glucose levels from the dissolved ear-wax solution, following manufacturer instructions.