A method for specifically detecting Babesia species nucleic acid in a sample, said method comprising: contacting a sample, said sample suspected of containing Babesia species nucleic acid, with amplification oligomers for amplifying a target region of a Babesia species target nucleic acid, wherein the amplification oligomers comprise: (a) a first amplification oligomer comprising a target-hybridizing sequence that comprises or consists of SEQ ID NO:8 and a second amplification oligomer comprising a target-hybridizing sequence that comprises or consists of SEQ ID NO:83; and either (b) a third amplification oligomer comprising a target-hybridizing sequence that comprises or consists of SEQ ID NO:84 or SEQ ID NO:86; or (c) a third amplification oligomer comprising a target-hybridizing sequence that comprises or consists of SEQ ID NO:34 and a fourth amplification oligomer comprising a target-hybridizing sequence that comprises or consists of SEQ ID NO:84 and a fifth amplification oligomer comprising a target-hybridizing sequence that comprises or consists of SEQ ID NO:86; performing an in vitro nucleic acid amplification reaction, wherein any Babesia target nucleic acid present in said sample is used as a template for generating an amplification product; and detecting the presence or absence of the amplification product, thereby indicating the presence or absence of Babesia species target nucleic acid in said sample. contacting a sample, said sample suspected of containing Babesia species nucleic acid, with amplification oligomers for amplifying a target region of a Babesia species target nucleic acid, wherein the amplification oligomers comprise: (a) a first amplification oligomer comprising a target-hybridizing sequence that comprises or consists of SEQ ID NO:8 and a second amplification oligomer comprising a target-hybridizing sequence that comprises or consists of SEQ ID NO:83; and either (b) a third amplification oligomer comprising a target-hybridizing sequence that comprises or consists of SEQ ID NO:84 or SEQ ID NO:86; or (c) a third amplification oligomer comprising a target-hybridizing sequence that comprises or consists of SEQ ID NO:34 and a fourth amplification oligomer comprising a target-hybridizing sequence that comprises or consists of SEQ ID NO:84 and a fifth amplification oligomer comprising a target-hybridizing sequence that comprises or consists of SEQ ID NO:86; (a) a first amplification oligomer comprising a target-hybridizing sequence that comprises or consists of SEQ ID NO:8 and a second amplification oligomer comprising a target-hybridizing sequence that comprises or consists of SEQ ID NO:83; and either (b) a third amplification oligomer comprising a target-hybridizing sequence that comprises or consists of SEQ ID NO:84 or SEQ ID NO:86; or (c) a third amplification oligomer comprising a target-hybridizing sequence that comprises or consists of SEQ ID NO:34 and a fourth amplification oligomer comprising a target-hybridizing sequence that comprises or consists of SEQ ID NO:84 and a fifth amplification oligomer comprising a target-hybridizing sequence that comprises or consists of SEQ ID NO:86; performing an in vitro nucleic acid amplification reaction, wherein any Babesia target nucleic acid present in said sample is used as a template for generating an amplification product; and detecting the presence or absence of the amplification product, thereby indicating the presence or absence of Babesia species target nucleic acid in said sample.