A method of preparing a sequencing library for sequencing a target nucleic acid without the use of nanodrops comprising: (a) transposing an insertion sequence into first fragments of the target nucleic acid, wherein the insertion sequence comprises a hybridization sequence, and wherein the transposing produces nicks in the first fragments and 3' hydroxyl at said nicks; (b) combining in a single mixture (i) the first fragments of the target nucleic acid from (a), (ii) a splint oligonucleotide, and (iii) a population of beads, wherein each bead comprises capture oligonucleotides immobilized thereon, said capture oligonucleotides comprising 1) a barcode-containing sequence, wherein the oligonucleotides immobilized on the same individual bead comprise the same barcode-containing sequence and a majority of beads have different barcode-containing sequences, 2) a common sequence complementary to at least a portion of the splint oligonucleotide, where a second portion of the splint oligonucleotide is complementary to at least a portion of the hybridization sequence; 3) a first PCR primer annealing site; (c) ligating capture oligonucleotides of individual beads to inserted hybridization sequences of individual first fragments to produce ligated products; (d) combining a 3' branch ligation (3'BL) adaptor with the ligated products of Step (c), wherein the 3'BL adaptor comprises a first oligonucleotide and a second oligonucleotide, annealed to form a blunt duplex end and a single-stranded DNA end, wherein the first oligonucleotide comprises a 5' phosphate at the blunt duplex end; ligating the first oligonucleotide to the first fragments at the nicks in the first fragments, wherein the ligating is a 3' branch ligation covalently joining the 5' phosphate of the first oligonucleotide to the 3' hydroxyl at the nicks in the first fragments, and wherein the first oligonucleotide comprises a second PCR primer annealing site. (a) transposing an insertion sequence into first fragments of the target nucleic acid, wherein the insertion sequence comprises a hybridization sequence, and wherein the transposing produces nicks in the first fragments and 3' hydroxyl at said nicks; (b) combining in a single mixture (i) the first fragments of the target nucleic acid from (a), (ii) a splint oligonucleotide, and (iii) a population of beads, wherein each bead comprises capture oligonucleotides immobilized thereon, said capture oligonucleotides comprising 1) a barcode-containing sequence, wherein the oligonucleotides immobilized on the same individual bead comprise the same barcode-containing sequence and a majority of beads have different barcode-containing sequences, 2) a common sequence complementary to at least a portion of the splint oligonucleotide, where a second portion of the splint oligonucleotide is complementary to at least a portion of the hybridization sequence; 3) a first PCR primer annealing site; 1) a barcode-containing sequence, wherein the oligonucleotides immobilized on the same individual bead comprise the same barcode-containing sequence and a majority of beads have different barcode-containing sequences, 2) a common sequence complementary to at least a portion of the splint oligonucleotide, where a second portion of the splint oligonucleotide is complementary to at least a portion of the hybridization sequence; 3) a first PCR primer annealing site; (c) ligating capture oligonucleotides of individual beads to inserted hybridization sequences of individual first fragments to produce ligated products; (d) combining a 3' branch ligation (3'BL) adaptor with the ligated products of Step (c), wherein the 3'BL adaptor comprises a first oligonucleotide and a second oligonucleotide, annealed to form a blunt duplex end and a single-stranded DNA end, wherein the first oligonucleotide comprises a 5' phosphate at the blunt duplex end; ligating the first oligonucleotide to the first fragments at the nicks in the first fragments, wherein the ligating is a 3' branch ligation covalently joining the 5' phosphate of the first oligonucleotide to the 3' hydroxyl at the nicks in the first fragments, and wherein the first oligonucleotide comprises a second PCR primer annealing site.