An automatic purification apparatus for isolating a target substance from a plurality of biological samples, comprising: - a pipette block 100 which is attached in two rows a plurality of pipettes 141, 142 for sucking and discharging a fluid material are removably installed; - fixed body 200 which supports the pipette block 100; - a up and down moving part which is fixed at the fixed body for up and down moving the pipette block 100; - a base plate 400 which is located at lower part of the fixed body 200 and which is mounted a multi-well plate kit 420 420'including a plurality of a unit well in two rows; - a magnetic field applying part 700 which is mounted on a base plate 400 so as to apply and release magnetic field to a particular unit well of a multi-well plate 420, 420' located at a lower side of the pipette block 100; wherein a solution drip tray 510 is disposed to be horizontally moved by a solution drip tray moving means installed at the fixed body 200 and thus located at a lower side of the plurality of pipettes 141 and 142 installed at the pipette block 100 when the pipette block 100 is moved horizontally; wherein the magnetic field applying part 700 comprises a magnet mounting part 710 on which a magnet 711 is mounted and which is located at a lower side of the particular unit well of the multi-well plate 420, 420'; and a lifting part 760 which lifts up and down the magnet mounting part 710 so as to apply and release magnetic field to the particular unit well of the multi-well plate 420, 420'; wherein a unit well inserting groove 713 is formed at an upper surface of the magnet mounting part 710 so that the lower portion of the particular unit well of the multi-well plate 420, 420' is inserted therein, and the base plate 400 is formed with a unit well exposing hole 400-3 so that the lower portion of the particular unit well of the multi-well plate 420, 420' is inserted in the unit well inserting groove 713 when the magnet mounting part 710 is lifted up; further comprising a heating part 810 for heating the particular unit well of a multi-well plate 420, 420'; wherein the magnetic field applying part 700 comprises a magnet mounting part 710 on which a magnet 711 is mounted and which is located at a lower side of the particular unit well of the multi-well plate 420, 420'; and a lifting part 760 which lifts up and down the magnet mounting part 710 so as to apply and release magnetic field to the particular unit well of the multi-well plate 420, 420', and the heating part 810 is installed at the magnet mounting part 710; wherein the heating part 810 is a heat generating film which is contacted with the magnet mounting part 710. - a pipette block 100 which is attached in two rows a plurality of pipettes 141, 142 for sucking and discharging a fluid material are removably installed; - fixed body 200 which supports the pipette block 100; - a up and down moving part which is fixed at the fixed body for up and down moving the pipette block 100; - a base plate 400 which is located at lower part of the fixed body 200 and which is mounted a multi-well plate kit 420 420'including a plurality of a unit well in two rows; - a magnetic field applying part 700 which is mounted on a base plate 400 so as to apply and release magnetic field to a particular unit well of a multi-well plate 420, 420' located at a lower side of the pipette block 100;