An ultrasound sub-surface probe microscopy device comprising: - a stage for carrying a sample; - a signal generator to generate a drive signal (Sdr); - a scanning head comprising an actuator configured to generate in response to said drive signal an ultrasound acoustic input signal (I ac) having at least one acoustic input signal component (I ac1) with a first angular frequency (ω1), and a tip to transmit the acoustic input signal through a tip-sample interface as an acoustic wave (W ac) into the sample, the tip-sample interface providing for a non-linear interaction resulting in at least one upmixed acoustic signal component (W ac2) in said acoustic wave having a second angular frequency (ω2) higher than said first angular frequency (ω1); - a signal processor to generate an image signal (Sim) in response to the sensor signal and; - a scanning mechanism to provide for a relative displacement between the sample and the scanning head, along the surface of the sample, wherein the scanning head further comprises a sensor facility to provide a sensor signal (Ssense) that is indicative for a contribution (W' ac2) of the at least one upmixed acoustic signal component in reflections (W' ac) of the acoustic wave within the sample, wherein the ultrasound sub-surface probe microscopy device further comprises a feedback unit to control the signal generator and/or a force-distance analytic module to control a property of said non-linear transmission by controlling a set point force exerted by the tip to the sample and characterized in that the sensor facility of the scanning head is configured to indirectly sense the contribution of said at least one upmixed acoustic signal component in said reflections by downmixing said at least one upmixed acoustic signal component with another upmixed acoustic signal component in a non-linear tip-sample interaction to at least one downmixed acoustic signal component with a third angular frequency lower than said second angular frequency, the another upmixed acoustic signal component originating from the first-mentioned non-linear tip-sample interaction in the tip-sample interface. - a stage for carrying a sample; - a signal generator to generate a drive signal (Sdr); - a scanning head comprising an actuator configured to generate in response to said drive signal an ultrasound acoustic input signal (I ac) having at least one acoustic input signal component (I ac1) with a first angular frequency (ω1), and a tip to transmit the acoustic input signal through a tip-sample interface as an acoustic wave (W ac) into the sample, the tip-sample interface providing for a non-linear interaction resulting in at least one upmixed acoustic signal component (W ac2) in said acoustic wave having a second angular frequency (ω2) higher than said first angular frequency (ω1); - a signal processor to generate an image signal (Sim) in response to the sensor signal and; - a scanning mechanism to provide for a relative displacement between the sample and the scanning head, along the surface of the sample, wherein the scanning head further comprises a sensor facility to provide a sensor signal (Ssense) that is indicative for a contribution (W' ac2) of the at least one upmixed acoustic signal component in reflections (W' ac) of the acoustic wave within the sample, wherein the ultrasound sub-surface probe microscopy device further comprises a feedback unit to control the signal generator and/or a force-distance analytic module to control a property of said non-linear transmission by controlling a set point force exerted by the tip to the sample and characterized in that the sensor facility of the scanning head is configured to indirectly sense the contribution of said at least one upmixed acoustic signal component in said reflections by downmixing said at least one upmixed acoustic signal component with another upmixed acoustic signal component in a non-linear tip-sample interaction to at least one downmixed acoustic signal component with a third angular frequency lower than said second angular frequency, the another upmixed acoustic signal component originating from the first-mentioned non-linear tip-sample interaction in the tip-sample interface.