A cartridge for performing immuno-PCR to detect and/or quantify one or more target analytes, and/or detecting and/or quantifying a nucleic acid, said cartridge comprising: a sample receiving chamber; a chamber comprising a matrix material that acts as a filter and/or a DNA binding agent; a temperature controlled channel or chamber; and a plurality of chambers containing reagents for performing immuno-PCR, wherein: said plurality of chambers comprises a chamber containing a capture antibody that binds the analyte that is to be detected; said plurality of chambers comprises a chamber containing a detection antibody where said detection antibody is optionally attached directly or indirectly to a signal DNA; said plurality of chambers comprises a chamber containing a PCR master mix; said plurality of chambers comprises a chamber containing primers for amplifying all or a region of said signal DNA; and said plurality of chambers comprises a chamber containing a probe for detecting all or a region of said signal DNA, wherein said sample receiving chamber, said chamber comprising a matrix material, said temperature controlled heating channel or chamber or a port into said temperature controlled channel or chamber, and said plurality of chambers are disposed around a central valve and selectively in fluid communication with a channel in said central valve, wherein said central valve is configured to accommodate a plunger that is capable of drawing fluid into or out of a chamber in fluid communication with said central valve. a sample receiving chamber; a chamber comprising a matrix material that acts as a filter and/or a DNA binding agent; a temperature controlled channel or chamber; and a plurality of chambers containing reagents for performing immuno-PCR, wherein: said plurality of chambers comprises a chamber containing a capture antibody that binds the analyte that is to be detected; said plurality of chambers comprises a chamber containing a detection antibody where said detection antibody is optionally attached directly or indirectly to a signal DNA; said plurality of chambers comprises a chamber containing a PCR master mix; said plurality of chambers comprises a chamber containing primers for amplifying all or a region of said signal DNA; and said plurality of chambers comprises a chamber containing a probe for detecting all or a region of said signal DNA, wherein said sample receiving chamber, said chamber comprising a matrix material, said temperature controlled heating channel or chamber or a port into said temperature controlled channel or chamber, and said plurality of chambers are disposed around a central valve and selectively in fluid communication with a channel in said central valve, wherein said central valve is configured to accommodate a plunger that is capable of drawing fluid into or out of a chamber in fluid communication with said central valve. said plurality of chambers comprises a chamber containing a capture antibody that binds the analyte that is to be detected; said plurality of chambers comprises a chamber containing a detection antibody where said detection antibody is optionally attached directly or indirectly to a signal DNA; said plurality of chambers comprises a chamber containing a PCR master mix; said plurality of chambers comprises a chamber containing primers for amplifying all or a region of said signal DNA; and said plurality of chambers comprises a chamber containing a probe for detecting all or a region of said signal DNA, wherein said sample receiving chamber, said chamber comprising a matrix material, said temperature controlled heating channel or chamber or a port into said temperature controlled channel or chamber, and said plurality of chambers are disposed around a central valve and selectively in fluid communication with a channel in said central valve, wherein said central valve is configured to accommodate a plunger that is capable of drawing fluid into or out of a chamber in fluid communication with said central valve.