mhurhangee/ep-ind-claims huggingface dataset:
A method of preparing a library of tagged nucleic acid fragments comprising: (a) contacting a population of cells comprising less than 15 cells directly with a lysis reagent to generate a cell lysate, wherein the lysis reagent comprises one or more proteases, and wherein the cell lysate contains a target nucleic acid; (b) inactivating the one or more proteases to form an inactivated cell lysate; and (c) directly applying at least one transposase and at least one transposon end composition containing a transferred strand to the inactivated cell lysate under conditions where the target nucleic acid and the transposon end composition undergo a transposition reaction to generate a mixture; wherein the target nucleic acid is double-stranded DNA; wherein: (i) the target nucleic acid is fragmented to generate a plurality of target nucleic acid fragments, and (ii) the transferred strand of the transposon end composition is joined to 5' ends of each of a plurality of the target nucleic acid fragments to generate a plurality of 5' tagged target nucleic acid fragments; wherein the target nucleic acid remains double-stranded DNA prior to applying the transposase and the transposon end composition in (c); wherein no DNA purification or amplification occurs between (a) and (c); and wherein (a), (b), and (c) are performed in a single reaction mixture. (a) contacting a population of cells comprising less than 15 cells directly with a lysis reagent to generate a cell lysate, wherein the lysis reagent comprises one or more proteases, and wherein the cell lysate contains a target nucleic acid; (b) inactivating the one or more proteases to form an inactivated cell lysate; and (c) directly applying at least one transposase and at least one transposon end composition containing a transferred strand to the inactivated cell lysate under conditions where the target nucleic acid and the transposon end composition undergo a transposition reaction to generate a mixture; wherein the target nucleic acid is double-stranded DNA; wherein: (i) the target nucleic acid is fragmented to generate a plurality of target nucleic acid fragments, and (ii) the transferred strand of the transposon end composition is joined to 5' ends of each of a plurality of the target nucleic acid fragments to generate a plurality of 5' tagged target nucleic acid fragments; (i) the target nucleic acid is fragmented to generate a plurality of target nucleic acid fragments, and (ii) the transferred strand of the transposon end composition is joined to 5' ends of each of a plurality of the target nucleic acid fragments to generate a plurality of 5' tagged target nucleic acid fragments; wherein the target nucleic acid remains double-stranded DNA prior to applying the transposase and the transposon end composition in (c); wherein no DNA purification or amplification occurs between (a) and (c); and wherein (a), (b), and (c) are performed in a single reaction mixture.
Method for controlling a process within a system, namely a grinding process in a grinding device, comprising the following steps: - detecting state variables (s t) of the system by sensors of the grinding device, namely a microphone and/or by means of an automatic measuring of the output product; - creating at least two process models, which each are created and continuously adapted by a computer based neural network, which each describe the effects of actuating actions (a t) on the state variables (s t) of the system, the structure of the at least two process models differing from one another; - controlling of the process within the system by executing actuating actions (a t) under consideration of predefined control objectives and the process model, which currently provides the best prediction for the process running in the system, characterized in that similar state variables (s t) of the system are grouped together and wherein at least one process model is created for each group, so that the number of different process models is reduced, whereby the classification of similar state variables of the system into groups takes place in a self-learning and self-optimising manner, taking into account cost functions which compare the costs of a grouping of state variables of the system into a group to the costs of creating a new group. - detecting state variables (s t) of the system by sensors of the grinding device, namely a microphone and/or by means of an automatic measuring of the output product; - creating at least two process models, which each are created and continuously adapted by a computer based neural network, which each describe the effects of actuating actions (a t) on the state variables (s t) of the system, the structure of the at least two process models differing from one another; - controlling of the process within the system by executing actuating actions (a t) under consideration of predefined control objectives and the process model, which currently provides the best prediction for the process running in the system,
A method for transmitting a Channel State Information-Reference Signal, CSI-RS, the method comprising: generating a CSI-RS sequence for an antenna port of a maximum of N, N being an integer larger than or equal to 4 antenna ports; mapping the CSI-RS sequence to Resource Elements, REs; and generating an Orthogonal Frequency Division Multiplexing, OFDM, signal including the CSI-RS sequence and transmitting the generated OFDM signal to a User Equipment, wherein, the step of the mapping the CSI-RS sequence to the REs includes that; the CSI-RS sequence is mapped to REs corresponding to one sub-carrier at every 12 sub-carriers for each antenna port with respect to two OFDM symbols within a sub-frame by which the CSI-RS is transmitted, characterized in that for ┌N/2┐ antenna port sets, each of which comprises different antenna ports and includes an M th, wherein M≤N, and M being an odd number, antenna port and an (M+1) th antenna port and is used as an antenna port set for transmission of the CSI-RS, the CSI-RSs of antenna ports within each of the antenna port sets are allocated to REs having the same time-frequency resource and are discriminated from each other by orthogonal codes, and the CSI-RS allocated for two different antenna port sets within a resource block are spaced apart from each other with an interval of 3 REs in the frequency axis, the CSI-RS allocation REs for the neighbouring antenna port sets are alternately arranged one-by-one and a difference between a first sub-frame and a second sub-frame in the order of antenna port sets is provided in the direction of the frequency axis. generating a CSI-RS sequence for an antenna port of a maximum of N, N being an integer larger than or equal to 4 antenna ports; mapping the CSI-RS sequence to Resource Elements, REs; and generating an Orthogonal Frequency Division Multiplexing, OFDM, signal including the CSI-RS sequence and transmitting the generated OFDM signal to a User Equipment, wherein, the step of the mapping the CSI-RS sequence to the REs includes that; the CSI-RS sequence is mapped to REs corresponding to one sub-carrier at every 12 sub-carriers for each antenna port with respect to two OFDM symbols within a sub-frame by which the CSI-RS is transmitted, characterized in that for ┌N/2┐ antenna port sets, each of which comprises different antenna ports and includes an M th, wherein M≤N, and M being an odd number, antenna port and an (M+1) th antenna port and is used as an antenna port set for transmission of the CSI-RS, the CSI-RSs of antenna ports within each of the antenna port sets are allocated to REs having the same time-frequency resource and are discriminated from each other by orthogonal codes, and the CSI-RS allocated for two different antenna port sets within a resource block are spaced apart from each other with an interval of 3 REs in the frequency axis, the CSI-RS allocation REs for the neighbouring antenna port sets are alternately arranged one-by-one and a difference between a first sub-frame and a second sub-frame in the order of antenna port sets is provided in the direction of the frequency axis.
A copolymer comprising ester groups and having at least one nonpolar segment P and at least one polar segment D, the polar segment D having at least 8 repeat units and the proportion by weight of dispersing repeat units in the polar segment D being at least 30%, based on the weight of the polar segment D, and the nonpolar segment P having a proportion by weight of dispersing repeat units of less than 20% by weight, based on the weight of the nonpolar segment P, characterized in that the copolymer comprising ester groups is obtainable by NMP (Nitroxide Mediated Polymerization), wherein the copolymer comprises repeat units derived from styrene monomers and repeat units derived from a methacrylate, and wherein the dispersing repeat units are derived from one or more ethylenically unsaturated, polar ester compounds of the formula in which R is hydrogen or methyl, X is oxygen, sulfur or an amino group of the formula -NH-or-NR a - in which R a is an alkyl radical having 1 to 40 carbon atoms, R 10 is a radical which comprises 2 to 1000 carbon atoms and has at least one heteroatom, R 11 and R 12 are each independently hydrogen or a group of the formula - COX'R 10' in which X' is oxygen or an amino group of the formula -NH- or-NR a' - in which R a' is an alkyl radical having 1 to 40 carbon atoms, and R 10' is a radical comprising 1 to 100 carbon atoms, and/or from heterocyclic vinyl compounds.
A room temperature curable organopolysiloxane composition for protecting electric/electronic parts, comprising: 100 parts by mass of an organopolysiloxane having a viscosity at 25°C of 20 to 1,000,000 mPa · s and having at least two silicon atom-bonded hydroxyl groups or silicon atom-bonded alkoxy groups in a molecule; 0.5 to 15 parts by mass of an alkoxysilane represented by the following general formula or a partial hydrolysis condensation product thereof: R 1 a Si(OR 2) (4-a) wherein R 1 is a monovalent hydrocarbon group having a carbon number of 1 to 12, R 2 is an alkyl group having a carbon number of 1 to 3, and "a" is an integer of 0 to 2; 0.001 to 0.5 parts by mass of a mercaptobenzothiazole based compound; 0.1 to 30 parts by mass of zinc oxide powder and/or zinc carbonate powder; and 0.1 to 10 parts by mass of a catalyst for condensation reactions. 100 parts by mass of an organopolysiloxane having a viscosity at 25°C of 20 to 1,000,000 mPa · s and having at least two silicon atom-bonded hydroxyl groups or silicon atom-bonded alkoxy groups in a molecule; 0.5 to 15 parts by mass of an alkoxysilane represented by the following general formula or a partial hydrolysis condensation product thereof: R 1 a Si(OR 2) (4-a) wherein R 1 is a monovalent hydrocarbon group having a carbon number of 1 to 12, R 2 is an alkyl group having a carbon number of 1 to 3, and "a" is an integer of 0 to 2; 0.001 to 0.5 parts by mass of a mercaptobenzothiazole based compound; 0.1 to 30 parts by mass of zinc oxide powder and/or zinc carbonate powder; and 0.1 to 10 parts by mass of a catalyst for condensation reactions.
A control apparatus for a hybrid electric vehicle including an electric motor (18, 20) configured to drive a driving wheel of the hybrid electric vehicle; a battery configured to supply an electric current to the electric motor (18, 20); a generator configured to supply an electric current to the electric motor (18, 20); and an engine configured to allow the generator to generate electric power, the control apparatus comprising: a temperature detector configured to detect a temperature of the battery; a temperature increase rate calculator configured to calculate, based on the detected temperature of the battery, a temperature increase rate (α) that is an amount of increase in the temperature per unit time (ΔTB/Δt); and characterised in that there is an engine controller (48, 48C, 48D) configured to allow, when the temperature increase rate is equal to or higher than a threshold value (α1), the engine to be started, so that the generator generates the electric power, wherein the threshold value (α1) is set so that the higher the detected battery temperature, the lower the threshold value, wherein the engine controller (48, 48C, 48D) controls the engine so that the higher the temperature increase rate, the larger an electric power generation amount of the generator. a temperature detector configured to detect a temperature of the battery; a temperature increase rate calculator configured to calculate, based on the detected temperature of the battery, a temperature increase rate (α) that is an amount of increase in the temperature per unit time (ΔTB/Δt); and characterised in that there is an engine controller (48, 48C, 48D) configured to allow, when the temperature increase rate is equal to or higher than a threshold value (α1), the engine to be started, so that the generator generates the electric power, wherein the threshold value (α1) is set so that the higher the detected battery temperature, the lower the threshold value, wherein the engine controller (48, 48C, 48D) controls the engine so that the higher the temperature increase rate, the larger an electric power generation amount of the generator.
A system for supplying hydraulic flow to a load, the system comprising: a pump; a flow amplifier for, when actuated, supplying fluid to the pump; and a flow control valve for controlling the flow of fluid to the load, fluid not directed to the load being directed to the flow amplifier to actuate the flow amplifier; wherein the flow control valve has an inlet connected to an outlet of the pump, an outlet for supplying fluid to the load to which hydraulic flow is to be supplied, and a bypass flow outlet connected to the flow amplifier for driving the flow amplifier for supplying fluid to an inlet of the pump. a pump; a flow amplifier for, when actuated, supplying fluid to the pump; and a flow control valve for controlling the flow of fluid to the load, fluid not directed to the load being directed to the flow amplifier to actuate the flow amplifier; wherein the flow control valve has an inlet connected to an outlet of the pump, an outlet for supplying fluid to the load to which hydraulic flow is to be supplied, and a bypass flow outlet connected to the flow amplifier for driving the flow amplifier for supplying fluid to an inlet of the pump.
An aqueous fabric conditioner composition comprising (a) from 2 to 9 wt % of a fabric softening active, by weight of the total composition, wherein the fabric softening active is an ester-linked quaternary ammonium compound having fatty acid chains comprising from 20 to 35 wt % of saturated C18 chains and from 20 to 35 wt % of monounsaturated C18 chains, by weight of total fatty acid chains; (b) from 0.01 to 0.5 wt %, by weight of the total composition, of a floc prevention agent, which is a non-ionic alkoxylated material having an HLB value of from 8 to 18; (c) at least 75 wt% water; and (d) fatty material selected from a fatty alcohol and a fatty acid, wherein the aqueous fabric conditioner composition has a viscosity of greater than 50 cps, preferably from 55 to 200 cps as measured on a cup and bob viscometer; the viscosity being continuously measured under shear at 106s -1 for 60 seconds, at 25°C and wherein the composition leads to little or no floc formation upon addition to water. (a) from 2 to 9 wt % of a fabric softening active, by weight of the total composition, wherein the fabric softening active is an ester-linked quaternary ammonium compound having fatty acid chains comprising from 20 to 35 wt % of saturated C18 chains and from 20 to 35 wt % of monounsaturated C18 chains, by weight of total fatty acid chains; (b) from 0.01 to 0.5 wt %, by weight of the total composition, of a floc prevention agent, which is a non-ionic alkoxylated material having an HLB value of from 8 to 18; (c) at least 75 wt% water; and (d) fatty material selected from a fatty alcohol and a fatty acid,
An exhaust gas aftertreatment device for an internal combustion engine, in particular for a diesel internal combustion engine for a ship operated with heavy fuel oil, having a housing; having an exhaust gas chamber defined by the housing and continuously flowed through by exhaust gas, into which exhaust gas flows via an inlet and out of which the exhaust gas flows via an outlet; having a sound-damping chamber that is defined by the housing and coupled to the exhaust gas chamber, in which a fluid or a free-flowing solid is received with a filling level, wherein the filling level is adjustable in such a manner that the same is dependent on the frequency of the exhaust gas sound to be dampened, characterized in that the filling level in the sound-damping chamber is adjustable via an inlet of the sound-damping chamber and an outlet of the sound-damping chamber.
Tank, intended for reversible storage of hydrogen, comprising: - several casings of cylindrical shape each containing hydrides and each suitable for being filled with or emptied of hydrogen H 2 to be absorbed or desorbed by the hydrides, respectively, - a solid component made of thermally insulating material with a low heat capacity with its interior perforated by several housings of cylindrical shape, the diameter of each being greater than that of a casing, in said tank, each casing is housed individually in a housing, leaving an annular space free between them so that the latter may be traversed by a heat-transfer fluid, along a circuit in each annular space defined from an inlet common to all the annular spaces to an outlet, which is also common. - several casings of cylindrical shape each containing hydrides and each suitable for being filled with or emptied of hydrogen H 2 to be absorbed or desorbed by the hydrides, respectively, - a solid component made of thermally insulating material with a low heat capacity with its interior perforated by several housings of cylindrical shape, the diameter of each being greater than that of a casing,
A gas dispenser for dispensing fuel gas to a vehicle, comprising: a bottom part adapted to be placed on a ground and comprising a dispensing unit connected to a gas tank and to at least a nozzle intended to supply gas to the vehicle, a top part arranged above the bottom part, and at least one intermediate part connecting the bottom part and the top part, characterised in that the top part comprises a gas collecting housing having at least one opening, through which fuel gas at a gaseous state can enter, and that a gas sensor is arranged at the gas collecting housing, the gas sensor being adapted to detect gas present in the gas collecting housing. a bottom part adapted to be placed on a ground and comprising a dispensing unit connected to a gas tank and to at least a nozzle intended to supply gas to the vehicle, a top part arranged above the bottom part, and at least one intermediate part connecting the bottom part and the top part, characterised in that the top part comprises a gas collecting housing having at least one opening, through which fuel gas at a gaseous state can enter, and that a gas sensor is arranged at the gas collecting housing, the gas sensor being adapted to detect gas present in the gas collecting housing.
An aft section structure of a jet engine having an axis, comprising: a casing defining a duct (9, 29) around the axis and opened axially fore and aft; a cone tapering aftward at a first angle (θ1) with the axis and having a pointed end; one or more guide vanes, each of the vanes radially extending from the cone to the casing and comprising a pressure side at a second angle (θ2) with a plane (Px) containing the axis; one or more spray bars, each of the spray bars extending radially within the duct (9, 29) and comprising one or more trailing sides (57, 59), each of the trailing sides (57, 59) being directed aftward at a third angle (θ3) with a plane (Px) containing the axis; and one or more flame holders, each of the flame holders extending radially within the duct (9, 29) and comprising one or more interior sides (61, 63), each of the interior sides (61, 63) being directed aftward at a fourth angle (θ4) with a plane (Px) containing the axis, wherein any of the first through fourth angles (θ1, θ2, θ3, θ4) is over 0 degree and less than 45 degrees, and wherein the first through fourth angles (θ1, θ2, θ3, θ4) are identical. a casing defining a duct (9, 29) around the axis and opened axially fore and aft; a cone tapering aftward at a first angle (θ1) with the axis and having a pointed end; one or more guide vanes, each of the vanes radially extending from the cone to the casing and comprising a pressure side at a second angle (θ2) with a plane (Px) containing the axis; one or more spray bars, each of the spray bars extending radially within the duct (9, 29) and comprising one or more trailing sides (57, 59), each of the trailing sides (57, 59) being directed aftward at a third angle (θ3) with a plane (Px) containing the axis; and one or more flame holders, each of the flame holders extending radially within the duct (9, 29) and comprising one or more interior sides (61, 63), each of the interior sides (61, 63) being directed aftward at a fourth angle (θ4) with a plane (Px) containing the axis, wherein any of the first through fourth angles (θ1, θ2, θ3, θ4) is over 0 degree and less than 45 degrees, and wherein the first through fourth angles (θ1, θ2, θ3, θ4) are identical.
A composition selected from (i) a composition comprising an ionizable lipid of formula I and a cationic lipid in a liposome comprising a bilayer of lipid molecules; and (ii) a composition comprising more than one ionizable lipid of formula I in a liposome comprising a bilayer of lipid molecules, wherein the ionizable lipid of formula I has the following structure: wherein n and m are independently 1, 2, 3, or 4; R 1 and R 2 are independently C 10-18 alkyl or C 12-18 alkenyl; X is -CH 2 -, S, O, or absent; L is C 1-4 alkylene, -S-C 1-4 alkylene, -O-C 1-4 alkylene, -O-C -C 1-4 alkylene, -S 2 -C 1-4 alkylene, or represents or a pharmaceutically acceptable salt form thereof. (i) a composition comprising an ionizable lipid of formula I and a cationic lipid in a liposome comprising a bilayer of lipid molecules; and (ii) a composition comprising more than one ionizable lipid of formula I in a liposome comprising a bilayer of lipid molecules, wherein n and m are independently 1, 2, 3, or 4; R 1 and R 2 are independently C 10-18 alkyl or C 12-18 alkenyl; X is -CH 2 -, S, O, or absent; L is C 1-4 alkylene, -S-C 1-4 alkylene, -O-C 1-4 alkylene, -O-C -C 1-4 alkylene, -S 2 -C 1-4 alkylene, or represents or a pharmaceutically acceptable salt form thereof.