A method for performing a nucleotide polymerization reaction or a nucleic acid sequencing reaction, comprising: contacting a modified Bst DNA polymerase or a biologically active fragment thereof with a nucleic acid template in the presence of one or more nucleotides, wherein the modified Bst DNA polymerase or the biologically active fragment thereof comprise at least 95% identity to SEQ ID NO: 2 and further comprises an amino acid substitution selected from the group consisting of D144M, D144K, E220K, N234R, N234K, V241K, D264Q, D264S, D264K, Y272R, L280R, E294S, E294F, E294G, E294K, I331Q, I354W, I354F, I370A, Q409R, V418M, V418I, D423S, D423K, D423N, D423R, D423T, D423G, D423I, D423K, G425R, F448K, N457T, A462T, D480R, D480N, D480H, D480A, D480S,D480Q, N485W, N485Y, N487H, N487W, N487F, N487I, V488R, E493Q, and W577Y; or two amino acid substitutions comprising E493Q and D264K; or three amino acid substitutions comprising E220K, N234R and D423K,, wherein the numbering is relative of the amino acid sequence of SEQ ID NO: 2 polymerizing at least one of the one or more nucleotides using the modified Bst DNA polymerase or the biologically active fragment thereof, and detecting a signal indicating the polymerization of the at least one of the one or more nucleotides by the modified Bst DNA polymerase or the biologically active fragment thereof using a sensor. contacting a modified Bst DNA polymerase or a biologically active fragment thereof with a nucleic acid template in the presence of one or more nucleotides, wherein the modified Bst DNA polymerase or the biologically active fragment thereof comprise at least 95% identity to SEQ ID NO: 2 and further comprises an amino acid substitution selected from the group consisting of D144M, D144K, E220K, N234R, N234K, V241K, D264Q, D264S, D264K, Y272R, L280R, E294S, E294F, E294G, E294K, I331Q, I354W, I354F, I370A, Q409R, V418M, V418I, D423S, D423K, D423N, D423R, D423T, D423G, D423I, D423K, G425R, F448K, N457T, A462T, D480R, D480N, D480H, D480A, D480S,D480Q, N485W, N485Y, N487H, N487W, N487F, N487I, V488R, E493Q, and W577Y; or two amino acid substitutions comprising E493Q and D264K; or three amino acid substitutions comprising E220K, N234R and D423K,, wherein the numbering is relative of the amino acid sequence of SEQ ID NO: 2 an amino acid substitution selected from the group consisting of D144M, D144K, E220K, N234R, N234K, V241K, D264Q, D264S, D264K, Y272R, L280R, E294S, E294F, E294G, E294K, I331Q, I354W, I354F, I370A, Q409R, V418M, V418I, D423S, D423K, D423N, D423R, D423T, D423G, D423I, D423K, G425R, F448K, N457T, A462T, D480R, D480N, D480H, D480A, D480S,D480Q, N485W, N485Y, N487H, N487W, N487F, N487I, V488R, E493Q, and W577Y; or two amino acid substitutions comprising E493Q and D264K; or three amino acid substitutions comprising E220K, N234R and D423K,, wherein the numbering is relative of the amino acid sequence of SEQ ID NO: 2 polymerizing at least one of the one or more nucleotides using the modified Bst DNA polymerase or the biologically active fragment thereof, and detecting a signal indicating the polymerization of the at least one of the one or more nucleotides by the modified Bst DNA polymerase or the biologically active fragment thereof using a sensor.