A water treatment separation membrane, obtainable by a method for manufacturing the water treatment separation membrane comprising: forming a polyamide active layer on a porous support; and forming either a single protective layer including a polymer obtained by polymerization of an amine group-containing compound, an epoxy group-containing compound and a fluoro-containing compound, and polyvinyl pyrrolidone, or forming a two-layer protective layer structure formed with a first protective layer including a polymer obtained by polymerizing an amine group-containing compound, an epoxy group-containing compound and a fluoro-containing compound, and a second protective layer formed on top of the fist protective layer through a separate coating process and including polyvinyl pyrrolidone, wherein the protective layer on the polyamide active layer is formed using an aqueous solution including 0.005% by weight to 7% by weight of polyvinyl pyrrolidone based on the whole aqueous solution, wherein a thickness of the protective layer is 0.5 nm to 1000 nm wherein the amine group-containing compound and the epoxy group-containing compound are included in a weight ratio of 10:1 to 1:1, wherein the amine group-containing compound is a compound represented by the following chemical formula 2: wherein in chemical formula 2, m is an integer of 0 to 10, n is an integer of 0 to 15, and o is an integer of 0 to 10, wherein the epoxy group-containing compound is a compound represented by the following chemical formula 3: wherein in chemical formula 3 R1 is hydrogen or a hydroxyl group; R2 to R5 are each independently hydrogen, a hydroxyl group or an alkoxyalkyl oxirane group; a is an integer of 0 to 150; b is an integer of 0 to 3; c is an integer of 0 to 5; wherein the fluoro-containing compound is selected from F(CF 2) 3 O[CF(CF 3)CF 2 O] 3 CF(CF 3)COF, CHF 2 CF 2 CF 2 CH 2 OCH 2 CHCH 2 O, CHF 2 CF 2 CF 2 CF 2 CH 2 OCH 2 CHCH 2 O, F(CH 2) 10 COOH, F(CH 2) 7 COCl, F(CH 2) 10 CH 2 CH 2 OH, F(CH 2) 8 CH 2 CH 2 OH, (CF 3) 2 CCH 3 COF, F(CF 2) 6 CH 2 CHCH 2 O, CF 3 CHCHCOCl, and F(CF 2) 6 CH 2 NH 2. forming a polyamide active layer on a porous support; and forming either a single protective layer including a polymer obtained by polymerization of an amine group-containing compound, an epoxy group-containing compound and a fluoro-containing compound, and polyvinyl pyrrolidone, or forming a two-layer protective layer structure formed with a first protective layer including a polymer obtained by polymerizing an amine group-containing compound, an epoxy group-containing compound and a fluoro-containing compound, and a second protective layer formed on top of the fist protective layer through a separate coating process and including polyvinyl pyrrolidone, wherein the protective layer on the polyamide active layer is formed using an aqueous solution including 0.005% by weight to 7% by weight of polyvinyl pyrrolidone based on the whole aqueous solution, wherein a thickness of the protective layer is 0.5 nm to 1000 nm wherein the amine group-containing compound and the epoxy group-containing compound are included in a weight ratio of 10:1 to 1:1, wherein the amine group-containing compound is a compound represented by the following chemical formula 2: wherein in chemical formula 2, m is an integer of 0 to 10, n is an integer of 0 to 15, and o is an integer of 0 to 10, wherein the epoxy group-containing compound is a compound represented by the following chemical formula 3: wherein in chemical formula 3 R1 is hydrogen or a hydroxyl group; R2 to R5 are each independently hydrogen, a hydroxyl group or an alkoxyalkyl oxirane group; a is an integer of 0 to 150; b is an integer of 0 to 3; c is an integer of 0 to 5; wherein the fluoro-containing compound is selected from F(CF 2) 3 O[CF(CF 3)CF 2 O] 3 CF(CF 3)COF, CHF 2 CF 2 CF 2 CH 2 OCH 2 CHCH 2 O, CHF 2 CF 2 CF 2 CF 2 CH 2 OCH 2 CHCH 2 O, F(CH 2) 10 COOH, F(CH 2) 7 COCl, F(CH 2) 10 CH 2 CH 2 OH, F(CH 2) 8 CH 2 CH 2 OH, (CF 3) 2 CCH 3 COF, F(CF 2) 6 CH 2 CHCH 2 O, CF 3 CHCHCOCl, and F(CF 2) 6 CH 2 NH 2.