A centrifugation device for separating substances from a body fluid and tissue using centrifugal force, the centrifugation device comprising: a separation container configured to receive, centrifuge, and clean a body fluid and tissue therein and rotate around a central rotation axis; a supply part connected to the separation container and configured to supply a cleaning solution to the separation container; a discharge part connected to the separation container and configured to receive substances separated by centrifugation; valves configured to control flows between the supply part, the discharge part, and the separation container; a driving device configured to rotate the separation container, the supply part, and the discharge part around the central rotation axis, the driving device being installed in a direction perpendicular to the separation container, the supply part, and the discharge part; and a controller configured to control an operation of the driving device so as to adjust a rotational velocity at which the separation container, the supply part, and the discharge part are rotated, the separation container comprising: a piston located outside in a radial direction based on the central rotation axis; and an elastic part placed on an outer side of the piston in the radial direction to push the piston in a direction opposite a centrifugal force direction, the centrifugation device being characterized in that it is configured so that: when a rotational velocity of the driving device increases, the piston of the separation container is moved in the centrifugal force direction, decreasing an inside pressure of the separation container, and a cleaning solution flows from the supply part to the separation container and fills the separation container, and when the rotational velocity of the driving device decreases, the elastic part moves the piston of the separation container in the direction opposite the centrifugal force direction, and separated substances in the separation container are discharged to the discharge part. a separation container configured to receive, centrifuge, and clean a body fluid and tissue therein and rotate around a central rotation axis; a supply part connected to the separation container and configured to supply a cleaning solution to the separation container; a discharge part connected to the separation container and configured to receive substances separated by centrifugation; valves configured to control flows between the supply part, the discharge part, and the separation container; a driving device configured to rotate the separation container, the supply part, and the discharge part around the central rotation axis, the driving device being installed in a direction perpendicular to the separation container, the supply part, and the discharge part; and a controller configured to control an operation of the driving device so as to adjust a rotational velocity at which the separation container, the supply part, and the discharge part are rotated, the separation container comprising: a piston located outside in a radial direction based on the central rotation axis; and an elastic part placed on an outer side of the piston in the radial direction to push the piston in a direction opposite a centrifugal force direction, a piston located outside in a radial direction based on the central rotation axis; and an elastic part placed on an outer side of the piston in the radial direction to push the piston in a direction opposite a centrifugal force direction, the centrifugation device being characterized in that it is configured so that: when a rotational velocity of the driving device increases, the piston of the separation container is moved in the centrifugal force direction, decreasing an inside pressure of the separation container, and a cleaning solution flows from the supply part to the separation container and fills the separation container, and when the rotational velocity of the driving device decreases, the elastic part moves the piston of the separation container in the direction opposite the centrifugal force direction, and separated substances in the separation container are discharged to the discharge part. when a rotational velocity of the driving device increases, the piston of the separation container is moved in the centrifugal force direction, decreasing an inside pressure of the separation container, and a cleaning solution flows from the supply part to the separation container and fills the separation container, and when the rotational velocity of the driving device decreases, the elastic part moves the piston of the separation container in the direction opposite the centrifugal force direction, and separated substances in the separation container are discharged to the discharge part.