A computer implemented method for calibrating a data set of a target analyte in a sample comprising: (a) providing a normalization coefficient for calibrating the data set; wherein the data set is obtained from a signal-generating process for the target analyte using a signal-generating means; wherein the data set comprises a plurality of data points comprising cycles of the signal-generating process and signal values at the cycles; wherein the normalization coefficient is provided by using a reference value, a reference cycle and the data set; wherein the reference cycle is selected from the cycles of the data set; wherein the reference value is a value arbitrarily determined from a real number except zero; wherein the normalization coefficient is provided by defining a relationship between the reference value and a signal value at a cycle of the data set corresponding to the reference cycle; wherein the signal-generating process comprises a plurality of signal-generating processes performed in different reaction vessels; wherein the data set comprises a plurality of data sets obtained from the plurality of signal-generating processes; wherein the normalization coefficient is provided for each data set of the plurality of data sets; wherein the normalization coefficient for each data set of the plurality of data sets is provided by using the identical reference cycle and the identical reference value; and (b) providing a calibrated data set by obtaining calibrated signal values by applying the normalization coefficient to the signal values of the data set; wherein each data set of the plurality of data sets is calibrated by applying the normalization coefficient for each data set. (a) providing a normalization coefficient for calibrating the data set; wherein the data set is obtained from a signal-generating process for the target analyte using a signal-generating means; wherein the data set comprises a plurality of data points comprising cycles of the signal-generating process and signal values at the cycles; wherein the normalization coefficient is provided by using a reference value, a reference cycle and the data set; wherein the reference cycle is selected from the cycles of the data set; wherein the reference value is a value arbitrarily determined from a real number except zero; wherein the normalization coefficient is provided by defining a relationship between the reference value and a signal value at a cycle of the data set corresponding to the reference cycle; wherein the signal-generating process comprises a plurality of signal-generating processes performed in different reaction vessels; wherein the data set comprises a plurality of data sets obtained from the plurality of signal-generating processes; wherein the normalization coefficient is provided for each data set of the plurality of data sets; wherein the normalization coefficient for each data set of the plurality of data sets is provided by using the identical reference cycle and the identical reference value; and (b) providing a calibrated data set by obtaining calibrated signal values by applying the normalization coefficient to the signal values of the data set; wherein each data set of the plurality of data sets is calibrated by applying the normalization coefficient for each data set.