A method for determining event zygosity of a soybean plant comprising an AAD-12 soybean event pDAB4468-0416 according to SEQ ID NO: 1 as present in representative seed deposited with American Type Culture Collection (ATCC) under Accession No. PTA-10442, said event comprising a transgene construct according to residues 2731-9121 of SEQ ID NO: 1 comprising an AAD-12 gene, said transgene construct being flanked by a 5' flanking soybean genomic DNA comprising residues 1-2730 of SEQ ID NO: 1 and a 3' flanking soybean genomic DNA comprising residues 9122-10212 of SEQ ID NO: 1, said method comprising: obtaining a DNA sample of genomic DNA from said soybean plant; producing a contacted sample by contacting said DNA sample with a. a first event primer and a second event primer, wherein said first event primer specifically binds said transgene construct according to residues 2731-9121 of SEQ ID NO: 1, said second event primer specifically binds said 5' soybean genomic flanking DNA comprising residues 1-2730 of SEQ ID NO: 1 or said 3' soybean genomic flanking DNA comprising residues 9122-10212 of SEQ ID NO: 1, and wherein said first event primer and said second event primer produce an event amplicon when subjected to TAQMAN PCR conditions, b. a reference forward primer and a reference reverse primer that produce a reference amplicon from an endogenous soybean reference gene when subjected to TAQMAN PCR conditions, c. a fluorescent event probe that hybridizes with said event amplicon, d. a fluorescent reference probe that hybridizes with said reference amplicon; subjecting said contacted sample to fluorescence-based endpoint TAQMAN PCR conditions; quantitating said fluorescent event probe that hybridized to said event amplicon; quantitating said fluorescent reference probe that hybridized to said reference amplicon; comparing amounts of hybridized fluorescent event probe to hybridized fluorescent reference probe; and determining zygosity of pDAB4468-0416 by comparing fluorescence ratios of hybridized fluorescent event probe and hybridized fluorescent reference probe. obtaining a DNA sample of genomic DNA from said soybean plant; producing a contacted sample by contacting said DNA sample with a. a first event primer and a second event primer, wherein said first event primer specifically binds said transgene construct according to residues 2731-9121 of SEQ ID NO: 1, said second event primer specifically binds said 5' soybean genomic flanking DNA comprising residues 1-2730 of SEQ ID NO: 1 or said 3' soybean genomic flanking DNA comprising residues 9122-10212 of SEQ ID NO: 1, and wherein said first event primer and said second event primer produce an event amplicon when subjected to TAQMAN PCR conditions, b. a reference forward primer and a reference reverse primer that produce a reference amplicon from an endogenous soybean reference gene when subjected to TAQMAN PCR conditions, c. a fluorescent event probe that hybridizes with said event amplicon, d. a fluorescent reference probe that hybridizes with said reference amplicon; a. a first event primer and a second event primer, wherein said first event primer specifically binds said transgene construct according to residues 2731-9121 of SEQ ID NO: 1, said second event primer specifically binds said 5' soybean genomic flanking DNA comprising residues 1-2730 of SEQ ID NO: 1 or said 3' soybean genomic flanking DNA comprising residues 9122-10212 of SEQ ID NO: 1, and wherein said first event primer and said second event primer produce an event amplicon when subjected to TAQMAN PCR conditions, b. a reference forward primer and a reference reverse primer that produce a reference amplicon from an endogenous soybean reference gene when subjected to TAQMAN PCR conditions, c. a fluorescent event probe that hybridizes with said event amplicon, d. a fluorescent reference probe that hybridizes with said reference amplicon; subjecting said contacted sample to fluorescence-based endpoint TAQMAN PCR conditions; quantitating said fluorescent event probe that hybridized to said event amplicon; quantitating said fluorescent reference probe that hybridized to said reference amplicon; comparing amounts of hybridized fluorescent event probe to hybridized fluorescent reference probe; and determining zygosity of pDAB4468-0416 by comparing fluorescence ratios of hybridized fluorescent event probe and hybridized fluorescent reference probe.