A nerve treatment device for forming a fluid containment field around at least a portion of an isolated segment of a nerve, the nerve treatment device comprising: an elongate body extending from a first endwall to a second endwall substantially opposite the first endwall, the elongate body comprising a top surface and having a longitudinal axis extending from the first endwall to the second endwall; and a containment chamber formed within the elongate body and extending from the first endwall to the second endwall, the containment chamber comprising a void volume intersecting the top surface to form an access area, the access area being configured to receive the isolated segment of the nerve into the containment chamber and the containment chamber being configured to substantially retain a volume of fluid within the void volume around at least a portion of the isolated segment of the nerve; wherein the first endwall comprises a first aperture opening into the containment chamber and the second endwall comprises a second aperture opening into the containment chamber, the first and second apertures being configured to retain first and second ends of the isolated segment of the nerve, respectively, and to form fluid seals around the first and second ends of the isolated segment of the nerve, respectively, characterized by that the first endwall comprises a first slit extending through or along the first endwall from the top surface to the first aperture and the second endwall comprises a second slit extending through or along the second endwall from the top surface to the second aperture, and at least a portion of the first endwall is flexible and configured to be biased, or the elongate body comprises a front wall, wherein the front wall is configured to be biased, in a manner that increases a first width between opposing edges of the first slit so that the nerve may be received through the first slit into the first aperture and wherein at least a portion of the second endwall is flexible and configured to be biased, or the front wall is configured to be biased, in a manner that increases a second width between opposing edges of the second slit so that the nerve may be received through the second slit into the second aperture. an elongate body extending from a first endwall to a second endwall substantially opposite the first endwall, the elongate body comprising a top surface and having a longitudinal axis extending from the first endwall to the second endwall; and a containment chamber formed within the elongate body and extending from the first endwall to the second endwall, the containment chamber comprising a void volume intersecting the top surface to form an access area, the access area being configured to receive the isolated segment of the nerve into the containment chamber and the containment chamber being configured to substantially retain a volume of fluid within the void volume around at least a portion of the isolated segment of the nerve; wherein the first endwall comprises a first aperture opening into the containment chamber and the second endwall comprises a second aperture opening into the containment chamber, the first and second apertures being configured to retain first and second ends of the isolated segment of the nerve, respectively, and to form fluid seals around the first and second ends of the isolated segment of the nerve, respectively, characterized by that the first endwall comprises a first slit extending through or along the first endwall from the top surface to the first aperture and the second endwall comprises a second slit extending through or along the second endwall from the top surface to the second aperture, and at least a portion of the first endwall is flexible and configured to be biased, or the elongate body comprises a front wall, wherein the front wall is configured to be biased, in a manner that increases a first width between opposing edges of the first slit so that the nerve may be received through the first slit into the first aperture and wherein at least a portion of the second endwall is flexible and configured to be biased, or the front wall is configured to be biased, in a manner that increases a second width between opposing edges of the second slit so that the nerve may be received through the second slit into the second aperture.