A cyclone separation device of a cyclone vacuum cleaner, comprising: a cyclone chamber, comprising: an air inlet, allowing an airflow to enter the cyclone chamber; an annular sidewall, forming an air receiving region therein communicating with the air inlet, and guiding the airflow to flow spirally in the air receiving region; and a dust collecting region and an air outlet, disposed at two ends of the cyclone chamber along an axis of the cyclone chamber, wherein the dust collecting region communicates with the air receiving region, and wherein the air outlet discharges the airflow; and an airflow guiding member, disposed at the dust collecting region, characterized in that said airflow guiding member comprises: an airflow guiding chamber; at least one airflow entry opening, communicating with the dust collecting region and the airflow guiding chamber, and allowing the airflow in the dust collecting region to enter the airflow guiding chamber; and an airflow discharge opening, communicating with the airflow guiding chamber, disposed on the axis of the cyclone chamber to face the air outlet and output the airflow from the airflow guiding chamber to form an air discharging region. a cyclone chamber, comprising: an air inlet, allowing an airflow to enter the cyclone chamber; an annular sidewall, forming an air receiving region therein communicating with the air inlet, and guiding the airflow to flow spirally in the air receiving region; and a dust collecting region and an air outlet, disposed at two ends of the cyclone chamber along an axis of the cyclone chamber, wherein the dust collecting region communicates with the air receiving region, and wherein the air outlet discharges the airflow; and an air inlet, allowing an airflow to enter the cyclone chamber; an annular sidewall, forming an air receiving region therein communicating with the air inlet, and guiding the airflow to flow spirally in the air receiving region; and a dust collecting region and an air outlet, disposed at two ends of the cyclone chamber along an axis of the cyclone chamber, wherein the dust collecting region communicates with the air receiving region, and wherein the air outlet discharges the airflow; and an airflow guiding member, disposed at the dust collecting region, characterized in that said airflow guiding member comprises: an airflow guiding chamber; at least one airflow entry opening, communicating with the dust collecting region and the airflow guiding chamber, and allowing the airflow in the dust collecting region to enter the airflow guiding chamber; and an airflow discharge opening, communicating with the airflow guiding chamber, disposed on the axis of the cyclone chamber to face the air outlet and output the airflow from the airflow guiding chamber to form an air discharging region. an airflow guiding chamber; at least one airflow entry opening, communicating with the dust collecting region and the airflow guiding chamber, and allowing the airflow in the dust collecting region to enter the airflow guiding chamber; and an airflow discharge opening, communicating with the airflow guiding chamber, disposed on the axis of the cyclone chamber to face the air outlet and output the airflow from the airflow guiding chamber to form an air discharging region.