A system for procuring bodily fluid samples with reduced contamination, the system comprising: a fluid collection device; and a fluid transfer device, the fluid transfer device including: a housing forming at least a portion of a sequestration chamber, the housing having an inlet configured to be fluidically coupled to a bodily fluid source and an outlet configured to be fluidically coupled to the fluid collection device, the fluid collection device exerting a suction force in at least a portion of the housing when fluidically coupled to the outlet; an actuator coupled to the housing, the actuator having a first configuration in which the inlet is in fluid communication with the sequestration chamber, and a second configuration in which the inlet is in fluid communication with the outlet and fluidically isolated from the sequestration chamber; and a flow controller disposed in the housing and defining a portion of the sequestration chamber, the flow controller having a first state in which the portion of the sequestration chamber has a first volume and a second state in which the portion of the sequestration chamber has a second volume greater than the first volume, the flow controller configured to transition from the first state to the second state in response to the suction force being exerted on a surface of the flow controller such that, when the actuator is in the first configuration, the increase in volume of the portion of the sequestration chamber is operable to draw an initial volume of bodily fluid from the inlet into the portion of the sequestration chamber, the actuator configured to be transitioned to the second configuration after the initial volume of bodily fluid is drawn into the sequestration chamber to sequester the sequestration chamber from the inlet, and allow a subsequent volume of bodily fluid to flow from the inlet to the outlet in response to the suction force. a fluid collection device; and a fluid transfer device, the fluid transfer device including: a housing forming at least a portion of a sequestration chamber, the housing having an inlet configured to be fluidically coupled to a bodily fluid source and an outlet configured to be fluidically coupled to the fluid collection device, the fluid collection device exerting a suction force in at least a portion of the housing when fluidically coupled to the outlet; an actuator coupled to the housing, the actuator having a first configuration in which the inlet is in fluid communication with the sequestration chamber, and a second configuration in which the inlet is in fluid communication with the outlet and fluidically isolated from the sequestration chamber; and a flow controller disposed in the housing and defining a portion of the sequestration chamber, the flow controller having a first state in which the portion of the sequestration chamber has a first volume and a second state in which the portion of the sequestration chamber has a second volume greater than the first volume, the flow controller configured to transition from the first state to the second state in response to the suction force being exerted on a surface of the flow controller such that, when the actuator is in the first configuration, the increase in volume of the portion of the sequestration chamber is operable to draw an initial volume of bodily fluid from the inlet into the portion of the sequestration chamber, the actuator configured to be transitioned to the second configuration after the initial volume of bodily fluid is drawn into the sequestration chamber to sequester the sequestration chamber from the inlet, and allow a subsequent volume of bodily fluid to flow from the inlet to the outlet in response to the suction force. a housing forming at least a portion of a sequestration chamber, the housing having an inlet configured to be fluidically coupled to a bodily fluid source and an outlet configured to be fluidically coupled to the fluid collection device, the fluid collection device exerting a suction force in at least a portion of the housing when fluidically coupled to the outlet; an actuator coupled to the housing, the actuator having a first configuration in which the inlet is in fluid communication with the sequestration chamber, and a second configuration in which the inlet is in fluid communication with the outlet and fluidically isolated from the sequestration chamber; and a flow controller disposed in the housing and defining a portion of the sequestration chamber, the flow controller having a first state in which the portion of the sequestration chamber has a first volume and a second state in which the portion of the sequestration chamber has a second volume greater than the first volume, the flow controller configured to transition from the first state to the second state in response to the suction force being exerted on a surface of the flow controller such that, when the actuator is in the first configuration, the increase in volume of the portion of the sequestration chamber is operable to draw an initial volume of bodily fluid from the inlet into the portion of the sequestration chamber, the actuator configured to be transitioned to the second configuration after the initial volume of bodily fluid is drawn into the sequestration chamber to sequester the sequestration chamber from the inlet, and allow a subsequent volume of bodily fluid to flow from the inlet to the outlet in response to the suction force.