A method of separating a compound of interest from a mixture, the method comprising: (a) providing a mixture containing the compound of interest; (b) introducing a portion of the mixture to a chromatographic system having a chromatographic column; and (c) eluting the separated compound of interest from the column, wherein the column has a stationary phase having the following structure (i): a b c (i) wherein: X is a chromatographic substrate containing silica, metal oxide, an inorganic-organic hybrid material, a group of block copolymers, or combinations thereof; W is selected from the group consisting of hydrogen and hydroxyl, wherein W is bound to the surface of X; Q is represented by the following structure: T is represented by one of the following structures: Z is a) a surface attachment group having the formula (B 1) x (R 5) y (R 6) z Si-, wherein x is an integer from 1-3, y is an integer from 0-2, z is an integer from 0-2, and x+y+z = 3; R 5 and R 6 are each independently selected from the group consisting of methyl, ethyl, n -propyl, iso -propyl, n -butyl, iso -butyl, tert -butyl, substituted or unsubstituted aryl, cyclic alkyl, branched alkyl, lower alkyl, a protected or deprotected alcohol, a zwitterion group and a siloxane bond; and B 1 is a siloxane bond; or b) an attachment to a surface organofunctional hybrid group through a direct carbon-carbon bond formation or through a heteroatom, ester, ether, thioether, amine, amide, imide, urea, carbonate, carbamate, heterocycle, triazole, or urethane linkage; or c) an adsorbed, surface group that is not covalently attached to the surface of the material; and b and c are positive numbers, 0.05 ≤ (b/c) ≤ 100, and a ≥ 0. (a) providing a mixture containing the compound of interest; (b) introducing a portion of the mixture to a chromatographic system having a chromatographic column; and (c) eluting the separated compound of interest from the column, wherein the column has a stationary phase having the following structure (i): a b c (i) wherein: X is a chromatographic substrate containing silica, metal oxide, an inorganic-organic hybrid material, a group of block copolymers, or combinations thereof; W is selected from the group consisting of hydrogen and hydroxyl, wherein W is bound to the surface of X; Q is represented by the following structure: T is represented by one of the following structures: Z is a) a surface attachment group having the formula (B 1) x (R 5) y (R 6) z Si-, wherein x is an integer from 1-3, y is an integer from 0-2, z is an integer from 0-2, and x+y+z = 3; R 5 and R 6 are each independently selected from the group consisting of methyl, ethyl, n -propyl, iso -propyl, n -butyl, iso -butyl, tert -butyl, substituted or unsubstituted aryl, cyclic alkyl, branched alkyl, lower alkyl, a protected or deprotected alcohol, a zwitterion group and a siloxane bond; and B 1 is a siloxane bond; or b) an attachment to a surface organofunctional hybrid group through a direct carbon-carbon bond formation or through a heteroatom, ester, ether, thioether, amine, amide, imide, urea, carbonate, carbamate, heterocycle, triazole, or urethane linkage; or c) an adsorbed, surface group that is not covalently attached to the surface of the material; and b and c are positive numbers, 0.05 ≤ (b/c) ≤ 100, and a ≥ 0. X is a chromatographic substrate containing silica, metal oxide, an inorganic-organic hybrid material, a group of block copolymers, or combinations thereof; W is selected from the group consisting of hydrogen and hydroxyl, wherein W is bound to the surface of X; Q is represented by the following structure: T is represented by one of the following structures: Z is a) a surface attachment group having the formula (B 1) x (R 5) y (R 6) z Si-, wherein x is an integer from 1-3, y is an integer from 0-2, z is an integer from 0-2, and x+y+z = 3; R 5 and R 6 are each independently selected from the group consisting of methyl, ethyl, n -propyl, iso -propyl, n -butyl, iso -butyl, tert -butyl, substituted or unsubstituted aryl, cyclic alkyl, branched alkyl, lower alkyl, a protected or deprotected alcohol, a zwitterion group and a siloxane bond; and B 1 is a siloxane bond; or b) an attachment to a surface organofunctional hybrid group through a direct carbon-carbon bond formation or through a heteroatom, ester, ether, thioether, amine, amide, imide, urea, carbonate, carbamate, heterocycle, triazole, or urethane linkage; or c) an adsorbed, surface group that is not covalently attached to the surface of the material; and a) a surface attachment group having the formula (B 1) x (R 5) y (R 6) z Si-, wherein x is an integer from 1-3, y is an integer from 0-2, z is an integer from 0-2, and x+y+z = 3; R 5 and R 6 are each independently selected from the group consisting of methyl, ethyl, n -propyl, iso -propyl, n -butyl, iso -butyl, tert -butyl, substituted or unsubstituted aryl, cyclic alkyl, branched alkyl, lower alkyl, a protected or deprotected alcohol, a zwitterion group and a siloxane bond; and B 1 is a siloxane bond; or b) an attachment to a surface organofunctional hybrid group through a direct carbon-carbon bond formation or through a heteroatom, ester, ether, thioether, amine, amide, imide, urea, carbonate, carbamate, heterocycle, triazole, or urethane linkage; or c) an adsorbed, surface group that is not covalently attached to the surface of the material; and b and c are positive numbers, 0.05 ≤ (b/c) ≤ 100, and a ≥ 0.