A disposable cartridge for preparing a sample fluid containing cells for analysis, the cartridge comprising: one or more parallel preparation units, each preparation unit comprising: one or more chambers enclosed between frangible seals and connected in series, wherein at least one of the one of more chambers has a pressable portion and wherein each chamber includes a respective substance and is configured for receiving an input fluid, performing a procedure affecting said fluid thereby generating an output fluid, and releasing said output fluid; wherein a first chamber of said one or more chambers is coupled to a first opening via a first channel with a preceding frangible seal, wherein the preceding frangible seal comprises a first seal and a second seal spaced apart from the first seal, the first seal being a frangible seal that is breakable by a carrier and the second seal being a flexible ring configured to seal around the carrier to prevent fluid leakage out of the first chamber; wherein a last chamber of said one or more chambers is coupled to a second opening with a succeeding frangible seal which prevents flow from the respective preparation unit via the second opening; and wherein the succeeding frangible seal is configured to be broken by pressure generated from pressure applied to the pressable portion; and wherein the one or more preparation units are coupled to a compartment for performing analysis of the respective output fluids conveyable via the second opening. one or more parallel preparation units, each preparation unit comprising: one or more chambers enclosed between frangible seals and connected in series, wherein at least one of the one of more chambers has a pressable portion and wherein each chamber includes a respective substance and is configured for receiving an input fluid, performing a procedure affecting said fluid thereby generating an output fluid, and releasing said output fluid; wherein a first chamber of said one or more chambers is coupled to a first opening via a first channel with a preceding frangible seal, wherein the preceding frangible seal comprises a first seal and a second seal spaced apart from the first seal, the first seal being a frangible seal that is breakable by a carrier and the second seal being a flexible ring configured to seal around the carrier to prevent fluid leakage out of the first chamber; wherein a last chamber of said one or more chambers is coupled to a second opening with a succeeding frangible seal which prevents flow from the respective preparation unit via the second opening; and wherein the succeeding frangible seal is configured to be broken by pressure generated from pressure applied to the pressable portion; and one or more chambers enclosed between frangible seals and connected in series, wherein at least one of the one of more chambers has a pressable portion and wherein each chamber includes a respective substance and is configured for receiving an input fluid, performing a procedure affecting said fluid thereby generating an output fluid, and releasing said output fluid; wherein a first chamber of said one or more chambers is coupled to a first opening via a first channel with a preceding frangible seal, wherein the preceding frangible seal comprises a first seal and a second seal spaced apart from the first seal, the first seal being a frangible seal that is breakable by a carrier and the second seal being a flexible ring configured to seal around the carrier to prevent fluid leakage out of the first chamber; wherein a last chamber of said one or more chambers is coupled to a second opening with a succeeding frangible seal which prevents flow from the respective preparation unit via the second opening; and wherein the succeeding frangible seal is configured to be broken by pressure generated from pressure applied to the pressable portion; and wherein the one or more preparation units are coupled to a compartment for performing analysis of the respective output fluids conveyable via the second opening.