A gas barrier film comprising: a resin film base material which is a polyolefin resin film, a polyester resin film or a polyamide resin film; a layer made of an aqueous coating agent which contains a hardener and having gas barrier properties and adhesiveness to the resin film base material, the layer made of the aqueous coating agent being formed on at least one surface of the resin film base material; and at least one layer made of an aqueous coating agent having a compounding ratio of a hardener smaller than that of the aqueous coating agent, the layer made of the aqueous coating agent being formed on the layer made of the aqueous coating agent, wherein: the aqueous coating agent contains an aqueous polyurethane resin having water solubility or water dispersibility, a water soluble polymer and an inorganic layered mineral, the solid content compounding ratios of the aqueous polyurethane resin, the water soluble polymer and the inorganic layered mineral to the total solid content of the aqueous coating agent are in the following ranges: aqueous polyurethane resin 5 to 80 mass% water soluble polymer 10 to 80 mass% inorganic layered mineral 8 to 25 mass%, the aqueous polyurethane resin is an aqueous polyurethane resin which contains a polyurethane resin having an acid group and a polyamine compound, and the aqueous coating agent contains the aqueous polyurethane resin having water solubility or water dispersibility, the water soluble polymer and the inorganic layered mineral. a resin film base material which is a polyolefin resin film, a polyester resin film or a polyamide resin film; a layer made of an aqueous coating agent which contains a hardener and having gas barrier properties and adhesiveness to the resin film base material, the layer made of the aqueous coating agent being formed on at least one surface of the resin film base material; and at least one layer made of an aqueous coating agent having a compounding ratio of a hardener smaller than that of the aqueous coating agent, the layer made of the aqueous coating agent being formed on the layer made of the aqueous coating agent, wherein: the aqueous coating agent contains an aqueous polyurethane resin having water solubility or water dispersibility, a water soluble polymer and an inorganic layered mineral, the solid content compounding ratios of the aqueous polyurethane resin, the water soluble polymer and the inorganic layered mineral to the total solid content of the aqueous coating agent are in the following ranges: aqueous polyurethane resin 5 to 80 mass% water soluble polymer 10 to 80 mass% inorganic layered mineral 8 to 25 mass%, the aqueous polyurethane resin is an aqueous polyurethane resin which contains a polyurethane resin having an acid group and a polyamine compound, and the aqueous coating agent contains the aqueous polyurethane resin having water solubility or water dispersibility, the water soluble polymer and the inorganic layered mineral. the aqueous coating agent contains an aqueous polyurethane resin having water solubility or water dispersibility, a water soluble polymer and an inorganic layered mineral, the solid content compounding ratios of the aqueous polyurethane resin, the water soluble polymer and the inorganic layered mineral to the total solid content of the aqueous coating agent are in the following ranges: aqueous polyurethane resin 5 to 80 mass% water soluble polymer 10 to 80 mass% inorganic layered mineral 8 to 25 mass%, the aqueous polyurethane resin is an aqueous polyurethane resin which contains a polyurethane resin having an acid group and a polyamine compound, and the aqueous coating agent contains the aqueous polyurethane resin having water solubility or water dispersibility, the water soluble polymer and the inorganic layered mineral.