A display defect detection method for a display screen, wherein the method comprises: obtaining a first image of a front side of a tested display screen, wherein the first image is shot when the tested display screen is in a solid-color display state, the solid-color display state being a state in which a display is lighted up and shows no image or in which a display is lighted up and shows a solid-color image, and the front side of the tested display screen is a side that is used to display an image; separately obtaining a pixel feature of each pixel in the display area of the tested display screen in the first image, wherein the pixel feature comprises a luminance value and/or a contrast; detecting whether at least a first candidate pixel, whose pixel feature belongs to a first value range corresponding to the pixel feature of a suspected defective pixel, exists in the display area of the tested display screen; if at least a first candidate pixel exists in the display area of the tested display screen, detecting whether a first candidate pixel block constituted by sequentially arranged first candidate pixels exists and whether a quantity of pixels in the first candidate pixel block is greater than a first preset threshold; and if the first candidate pixel block exists, and if the first candidate pixel block is greater than a first preset threshold, identifying each first candidate pixel in the first candidate pixel block as a suspected defective pixel; obtaining a second image of the front side of the tested display screen, wherein the second image is shot when the tested display screen is in a die-out state and the front side of the tested display screen is illuminated by a diffuse reflection light source; identifying if at least one external smudgy pixel from the second image exists, whose location corresponds to at least one external smudge attached on the front side of the tested display screen, the at least one external smudgy pixel is a pixel whose pixel feature belongs to a second value range, the pixel feature of the external smudgy pixel comprising a luminance value and/or a contrast of the pixel in the display area of the tested display screen in the second image; detecting, for each suspected defective pixel identified from the first image, whether a pixel at a same location in the second image is an external smudgy pixel; and determining each suspected defective pixel as a display defective pixel if the pixel at the same location in the second image is not an external smudgy pixel. obtaining a first image of a front side of a tested display screen, wherein the first image is shot when the tested display screen is in a solid-color display state, the solid-color display state being a state in which a display is lighted up and shows no image or in which a display is lighted up and shows a solid-color image, and the front side of the tested display screen is a side that is used to display an image; separately obtaining a pixel feature of each pixel in the display area of the tested display screen in the first image, wherein the pixel feature comprises a luminance value and/or a contrast; detecting whether at least a first candidate pixel, whose pixel feature belongs to a first value range corresponding to the pixel feature of a suspected defective pixel, exists in the display area of the tested display screen; if at least a first candidate pixel exists in the display area of the tested display screen, detecting whether a first candidate pixel block constituted by sequentially arranged first candidate pixels exists and whether a quantity of pixels in the first candidate pixel block is greater than a first preset threshold; and if the first candidate pixel block exists, and if the first candidate pixel block is greater than a first preset threshold, identifying each first candidate pixel in the first candidate pixel block as a suspected defective pixel; obtaining a second image of the front side of the tested display screen, wherein the second image is shot when the tested display screen is in a die-out state and the front side of the tested display screen is illuminated by a diffuse reflection light source; identifying if at least one external smudgy pixel from the second image exists, whose location corresponds to at least one external smudge attached on the front side of the tested display screen, the at least one external smudgy pixel is a pixel whose pixel feature belongs to a second value range, the pixel feature of the external smudgy pixel comprising a luminance value and/or a contrast of the pixel in the display area of the tested display screen in the second image; detecting, for each suspected defective pixel identified from the first image, whether a pixel at a same location in the second image is an external smudgy pixel; and determining each suspected defective pixel as a display defective pixel if the pixel at the same location in the second image is not an external smudgy pixel.