An imaging system comprising: a microscope objective; first and second imaging channels (139, 140); a beam splitter and filter assembly that directs different wavelengths of light to the first imaging channel and the second imaging channel; and a sample positioning X-Y stage, the X-Y stage being independently rotatable in a plane normal to the axis of rotational symmetry and independently translatable in X and Y in the plane normal to the axis; wherein the first and the second imaging channel (139, 140) each contain: a time delay integration camera configured to produce an image from scanning of a sample comprising fluorescently tagged biomolecules along a scanning axis normal to an axis of rotational symmetry of the microscope objective; a lateral offset plate along the axis of rotational symmetry, the lateral offset plate configured to independently shift the position of the image in the time delay integration camera in a plane normal to the scanning axis; a zoom lens system comprising a tube lens and a helper lens configured to change a scale of the image directed to the time delay integration camera along the axis of rotational symmetry; and a rotational stage that supports the time delay integration camera, the rotational stage being rotatable around the axis of rotational symmetry. a microscope objective; first and second imaging channels (139, 140); a beam splitter and filter assembly that directs different wavelengths of light to the first imaging channel and the second imaging channel; and a sample positioning X-Y stage, the X-Y stage being independently rotatable in a plane normal to the axis of rotational symmetry and independently translatable in X and Y in the plane normal to the axis; wherein the first and the second imaging channel (139, 140) each contain: a time delay integration camera configured to produce an image from scanning of a sample comprising fluorescently tagged biomolecules along a scanning axis normal to an axis of rotational symmetry of the microscope objective; a lateral offset plate along the axis of rotational symmetry, the lateral offset plate configured to independently shift the position of the image in the time delay integration camera in a plane normal to the scanning axis; a zoom lens system comprising a tube lens and a helper lens configured to change a scale of the image directed to the time delay integration camera along the axis of rotational symmetry; and a rotational stage that supports the time delay integration camera, the rotational stage being rotatable around the axis of rotational symmetry. a time delay integration camera configured to produce an image from scanning of a sample comprising fluorescently tagged biomolecules along a scanning axis normal to an axis of rotational symmetry of the microscope objective; a lateral offset plate along the axis of rotational symmetry, the lateral offset plate configured to independently shift the position of the image in the time delay integration camera in a plane normal to the scanning axis; a zoom lens system comprising a tube lens and a helper lens configured to change a scale of the image directed to the time delay integration camera along the axis of rotational symmetry; and a rotational stage that supports the time delay integration camera, the rotational stage being rotatable around the axis of rotational symmetry.