An autofocusing system for a microscope, the autofocusing system being adapted to perform an autofocusing procedure, the autofocusing procedure comprising - predefining a set of target points for autofocusing, wherein at least one static rule or at least one dynamic rule exchangeable by a different rule is associated to each of the predefined target points or to a subset thereof, - the at least one rule defining at least one initial setting and at least one adaptation instruction for at least one parameter influencing how an autofocusing operation is performed for the target point, - the at least one adaptation instruction comprising at least one of an adaptation of the at least one parameter, an exchange of the at least one parameter, a substitution of the at least one rule by at least one different rule, and a deactivation of the at least one rule, and - the at least one parameter including at least one of a position or position change of the target point, an activation or deactivation pattern of the autofocusing operation for the target point, a frequency in which the target point is subjected to the autofocusing operation, at least one light parameter used in the autofocusing operation, and the autofocusing method used in the autofocusing operation, - initiating for each of the predefined set of target points or a subset thereof an initial autofocusing operation comprising the at least one initial setting as defined by the at least one rule associated to the target point, - initiating for each of the predefined set of target points or a subset thereof an adapted autofocusing operation based on the at least one adaptation instruction as defined by the at least one rule associated to the target point, and - further processing an autofocus value for at least some of the target points, the autofocus value being obtained by the initial autofocusing operation or by the adapted autofocusing operation. - predefining a set of target points for autofocusing, wherein at least one static rule or at least one dynamic rule exchangeable by a different rule is associated to each of the predefined target points or to a subset thereof, - the at least one rule defining at least one initial setting and at least one adaptation instruction for at least one parameter influencing how an autofocusing operation is performed for the target point, - the at least one adaptation instruction comprising at least one of an adaptation of the at least one parameter, an exchange of the at least one parameter, a substitution of the at least one rule by at least one different rule, and a deactivation of the at least one rule, and - the at least one parameter including at least one of a position or position change of the target point, an activation or deactivation pattern of the autofocusing operation for the target point, a frequency in which the target point is subjected to the autofocusing operation, at least one light parameter used in the autofocusing operation, and the autofocusing method used in the autofocusing operation, - initiating for each of the predefined set of target points or a subset thereof an initial autofocusing operation comprising the at least one initial setting as defined by the at least one rule associated to the target point, - initiating for each of the predefined set of target points or a subset thereof an adapted autofocusing operation based on the at least one adaptation instruction as defined by the at least one rule associated to the target point, and - further processing an autofocus value for at least some of the target points, the autofocus value being obtained by the initial autofocusing operation or by the adapted autofocusing operation.