A method for the determination of the content of an analyte in a sample comprising the following steps: a. providing at least one analysis solution, i. wherein the at least one analysis solution comprises each of an enzyme, a signal-generating substrate and a known portion of the sample to be analyzed, and ii. wherein the enzyme is able to convert both, the analyte and also the signal-generating substrate, so that analyte and signal-generating substrate compete for the conversion by the enzyme, b. detection of two signals S1 and S2 generated by enzyme-catalyzed conversion in the at least one analysis solution, wherein the signals are generated i. by enzyme-catalyzed conversion of a higher concentration of the signal-generating substrate (signal S1) and by enzyme-catalyzed conversion of a lower concentration of the signal-generating substrate (signal S2) in two separate analysis solutions, or ii. by enzyme-catalyzed conversion of the signal-generating substrate (signal S1) and by enzyme-catalyzed conversion of the analyte (signal S2), or iii. by enzyme-catalyzed conversion of a first signal-generating substrate (signal S1) and by enzyme-catalyzed conversion of a second signal-generating substrate (signal S2), c. calculation of a conversion factor from the signals, wherein the conversion factor is calculated as quotient of the initial rates v 0 /v 0 or as its inverse value v 0 /v 0, and d. determination of the content of the analyte in the sample with the help of the conversion factor. a. providing at least one analysis solution, i. wherein the at least one analysis solution comprises each of an enzyme, a signal-generating substrate and a known portion of the sample to be analyzed, and ii. wherein the enzyme is able to convert both, the analyte and also the signal-generating substrate, so that analyte and signal-generating substrate compete for the conversion by the enzyme, i. wherein the at least one analysis solution comprises each of an enzyme, a signal-generating substrate and a known portion of the sample to be analyzed, and ii. wherein the enzyme is able to convert both, the analyte and also the signal-generating substrate, so that analyte and signal-generating substrate compete for the conversion by the enzyme, b. detection of two signals S1 and S2 generated by enzyme-catalyzed conversion in the at least one analysis solution, wherein the signals are generated i. by enzyme-catalyzed conversion of a higher concentration of the signal-generating substrate (signal S1) and by enzyme-catalyzed conversion of a lower concentration of the signal-generating substrate (signal S2) in two separate analysis solutions, or ii. by enzyme-catalyzed conversion of the signal-generating substrate (signal S1) and by enzyme-catalyzed conversion of the analyte (signal S2), or iii. by enzyme-catalyzed conversion of a first signal-generating substrate (signal S1) and by enzyme-catalyzed conversion of a second signal-generating substrate (signal S2), i. by enzyme-catalyzed conversion of a higher concentration of the signal-generating substrate (signal S1) and by enzyme-catalyzed conversion of a lower concentration of the signal-generating substrate (signal S2) in two separate analysis solutions, or ii. by enzyme-catalyzed conversion of the signal-generating substrate (signal S1) and by enzyme-catalyzed conversion of the analyte (signal S2), or iii. by enzyme-catalyzed conversion of a first signal-generating substrate (signal S1) and by enzyme-catalyzed conversion of a second signal-generating substrate (signal S2), c. calculation of a conversion factor from the signals, wherein the conversion factor is calculated as quotient of the initial rates v 0 /v 0 or as its inverse value v 0 /v 0, and d. determination of the content of the analyte in the sample with the help of the conversion factor.