A system for regional citrate anticoagulation in an extracorporeal blood circuit including an arterial blood line arranged to be connected to a vascular access for withdrawing blood from a patient and a venous blood line arranged to be connected to the vascular access for returning blood to the patient, the system comprising: a blood pump operably connected to the arterial blood line; a hemofilter in fluid communication with the arterial and venous blood lines; a first pre-filter infusion line and pump connected to the arterial blood line upstream from the hemofilter for infusing a first pre-filter infusion solution from a first pre-filter source comprising a citrate anticoagulant-containing solution into blood in the arterial blood line, wherein the first pre-filter infusion solution contains dextrose; a second pre-filter infusion line and pump connected to the arterial blood line upstream from the hemofilter for infusing a second pre-filter infusion solution from a second pre-filter source comprising a first bicarbonate-containing solution into blood in the arterial blood line; a post-filter infusion line and pump connected to the venous blood line downstream from the hemofilter for infusing a post-filter infusion solution from a post-filter source comprising a second bicarbonate-containing solution into blood in the venous blood line; an effluent fluid line and ultrafiltration pump in fluid communication with the hemofilter for receiving a filter effluent fluid from the hemofilter; an additional infusion line and pump connected to the venous blood line downstream from a connection of the post-filter infusion line for infusing an additional infusion solution from an additional source comprising a calcium- and magnesium-containing solution into blood in the venous blood line; a dextrose sensor operably connected to the effluent fluid line for measuring dextrose concentration in the filter effluent fluid; and a control program for coordinating and monitoring the operation of the system and using data from the dextrose sensor to indicate citrate removal on the hemofilter. a blood pump operably connected to the arterial blood line; a hemofilter in fluid communication with the arterial and venous blood lines; a first pre-filter infusion line and pump connected to the arterial blood line upstream from the hemofilter for infusing a first pre-filter infusion solution from a first pre-filter source comprising a citrate anticoagulant-containing solution into blood in the arterial blood line, wherein the first pre-filter infusion solution contains dextrose; a second pre-filter infusion line and pump connected to the arterial blood line upstream from the hemofilter for infusing a second pre-filter infusion solution from a second pre-filter source comprising a first bicarbonate-containing solution into blood in the arterial blood line; a post-filter infusion line and pump connected to the venous blood line downstream from the hemofilter for infusing a post-filter infusion solution from a post-filter source comprising a second bicarbonate-containing solution into blood in the venous blood line; an effluent fluid line and ultrafiltration pump in fluid communication with the hemofilter for receiving a filter effluent fluid from the hemofilter; an additional infusion line and pump connected to the venous blood line downstream from a connection of the post-filter infusion line for infusing an additional infusion solution from an additional source comprising a calcium- and magnesium-containing solution into blood in the venous blood line; a dextrose sensor operably connected to the effluent fluid line for measuring dextrose concentration in the filter effluent fluid; and a control program for coordinating and monitoring the operation of the system and using data from the dextrose sensor to indicate citrate removal on the hemofilter.