A method for producing a secreted polypeptide, comprising: (a) cultivating a Gram-positive host cell in a medium conducive for the production of the polypeptide, wherein the host cell comprises a nucleic acid construct comprising a first nucleotide sequence encoding a signal peptide operably linked to a second nucleotide sequence encoding the polypeptide, wherein the first nucleotide sequence is foreign to the second nucleotide sequence, the 3' end of the first nucleotide sequence is immediately upstream of the second nucleotide sequence, and the first nucleotide sequence is selected from the group consisting of: (i) a nucleotide sequence encoding a signal peptide having an amino acid sequence which has at least 80% identity with SEQ ID NO:1; (ii) a nucleotide sequence having at least 80% identity with the sequence shown in positions 575 to 661 of SEQ ID NO: 10; and (iii) a nucleotide sequence which hybridizes under stringency conditions with a polynucleotide having the nucleotide sequence shown in positions 575-661 of SEQ ID NO: 10, or its complementary strand, wherein the stringency conditions are defined as prehybridization, hybridization, and washing post-hybridization at 5°C to 10°C below the calculated Tm in 0.9 M NaCl, 0.09 M Tris-HCl pH 7.6, 6 mM EDTA, 0.5% NP-40, 1X Denhardt's solution, 1 mM sodium pyrophosphate, 1 mM sodium monobasic phosphate, 0.1 mM ATP, and 0.2 mg of yeast RNA per ml, and washing once in 6X SCC plus 0.1 % SDS for 15 minutes and twice each for 15 minutes using 6X SSC at 5°C to 10°C below the calculated Tm; and optionally (b) isolating the secreted polypeptide from the cultivation medium. (a) cultivating a Gram-positive host cell in a medium conducive for the production of the polypeptide, wherein the host cell comprises a nucleic acid construct comprising a first nucleotide sequence encoding a signal peptide operably linked to a second nucleotide sequence encoding the polypeptide, wherein the first nucleotide sequence is foreign to the second nucleotide sequence, the 3' end of the first nucleotide sequence is immediately upstream of the second nucleotide sequence, and the first nucleotide sequence is selected from the group consisting of: (i) a nucleotide sequence encoding a signal peptide having an amino acid sequence which has at least 80% identity with SEQ ID NO:1; (ii) a nucleotide sequence having at least 80% identity with the sequence shown in positions 575 to 661 of SEQ ID NO: 10; and (iii) a nucleotide sequence which hybridizes under stringency conditions with a polynucleotide having the nucleotide sequence shown in positions 575-661 of SEQ ID NO: 10, or its complementary strand, wherein the stringency conditions are defined as prehybridization, hybridization, and washing post-hybridization at 5°C to 10°C below the calculated Tm in 0.9 M NaCl, 0.09 M Tris-HCl pH 7.6, 6 mM EDTA, 0.5% NP-40, 1X Denhardt's solution, 1 mM sodium pyrophosphate, 1 mM sodium monobasic phosphate, 0.1 mM ATP, and 0.2 mg of yeast RNA per ml, and washing once in 6X SCC plus 0.1 % SDS for 15 minutes and twice each for 15 minutes using 6X SSC at 5°C to 10°C below the calculated Tm; and optionally (i) a nucleotide sequence encoding a signal peptide having an amino acid sequence which has at least 80% identity with SEQ ID NO:1; (ii) a nucleotide sequence having at least 80% identity with the sequence shown in positions 575 to 661 of SEQ ID NO: 10; and (iii) a nucleotide sequence which hybridizes under stringency conditions with a polynucleotide having the nucleotide sequence shown in positions 575-661 of SEQ ID NO: 10, or its complementary strand, wherein the stringency conditions are defined as prehybridization, hybridization, and washing post-hybridization at 5°C to 10°C below the calculated Tm in 0.9 M NaCl, 0.09 M Tris-HCl pH 7.6, 6 mM EDTA, 0.5% NP-40, 1X Denhardt's solution, 1 mM sodium pyrophosphate, 1 mM sodium monobasic phosphate, 0.1 mM ATP, and 0.2 mg of yeast RNA per ml, and washing once in 6X SCC plus 0.1 % SDS for 15 minutes and twice each for 15 minutes using 6X SSC at 5°C to 10°C below the calculated Tm; and optionally (b) isolating the secreted polypeptide from the cultivation medium.