Operating and locking mechanism for crossings of central rail-guided vehicles, in which the crossing comprises a fixed part, adapted to be embedded in the surface of the street, a pivoting moving panel on which there is provided a guide rail, a fixed guide rail forming a first route with a fixed guide rail, a fixed guide rail forming a second route with a fixed guide rail, the rails (3A, 3B, 4C, 4D) being attached to the fixed part, such that the moving panel is circularly symmetric and rotates about its theoretical center, such that the moving panel selectively and alternatively allows passage through the first route or through the second route, when the guide rail of the moving panel is selectively aligned with the fixed rails (3A, 3B) of the first route or with the fixed rails (4C, 4D) of the second route, characterized in that the mechanism comprises: - a sliding plate adapted to be fixed to the fixed part, forming a channel in its central area in the direction of an axis, wherein the direction of the axis is parallel to the bisector corresponding to an angle to be formed by the first route and the second route of the crossing, - a guide block fixed to the sliding plate, comprising four guide grooves (10A, 10B, 10C, 10D) in the form of circular sectors located on the main plane of the guide block, the geometric center of which, in use, coincides with the theoretical pivoting point of the moving panel, where the sliding plate and the guide block form a rectangular section groove the axis of which is parallel to the direction of the axis, - four shafts (12A, 12B, 13A, 13B) to be, in use, fixed to the moving panel symmetrically with respect to the guide rail and perpendicular to the main plane of the moving panel, comprising respective first rollers (12E, 12F, 13E, 13F) that can be moved and rolled respectively within the guide grooves (10A, 10B, 10C, 10D), and - a cam plate sliding in the longitudinal direction within the groove formed by the sliding plate and the guide block, when in use, and comprising four grooves (14A, 14B, 14C, 14D) located on the main plane of the plate within which second rollers (12C, 12D, 13C, 13D) which are attached and rotate respectively on shafts (12A, 12B, 13A, 13B) can be moved and rolled respectively, said second rollers (12C, 12D, 13C, 13D) being at a lower level with respect to the first rollers (12E, 12F, 13E, 13F) where the cam plate is moved in the direction of the axis by a control motor through linear movement in one direction or the other of an operating tie rod, when in use, such that the linear movement in one direction or the other of the control motor through the control tie rod is converted into rotational movement in one direction or the other of the moving panel around the point, such that in the end positions thereof the moving panel is locked and the guide rail is aligned in a secure manner selectively with either the first route or with the second route. - a sliding plate adapted to be fixed to the fixed part, forming a channel in its central area in the direction of an axis, wherein the direction of the axis is parallel to the bisector corresponding to an angle to be formed by the first route and the second route of the crossing, - a guide block fixed to the sliding plate, comprising four guide grooves (10A, 10B, 10C, 10D) in the form of circular sectors located on the main plane of the guide block, the geometric center of which, in use, coincides with the theoretical pivoting point of the moving panel, where the sliding plate and the guide block form a rectangular section groove the axis of which is parallel to the direction of the axis, - four shafts (12A, 12B, 13A, 13B) to be, in use, fixed to the moving panel symmetrically with respect to the guide rail and perpendicular to the main plane of the moving panel, comprising respective first rollers (12E, 12F, 13E, 13F) that can be moved and rolled respectively within the guide grooves (10A, 10B, 10C, 10D), and - a cam plate sliding in the longitudinal direction within the groove formed by the sliding plate and the guide block, when in use, and comprising four grooves (14A, 14B, 14C, 14D) located on the main plane of the plate within which second rollers (12C, 12D, 13C, 13D) which are attached and rotate respectively on shafts (12A, 12B, 13A, 13B) can be moved and rolled respectively, said second rollers (12C, 12D, 13C, 13D) being at a lower level with respect to the first rollers (12E, 12F, 13E, 13F) where the cam plate is moved in the direction of the axis by a control motor through linear movement in one direction or the other of an operating tie rod, when in use, such that the linear movement in one direction or the other of the control motor through the control tie rod is converted into rotational movement in one direction or the other of the moving panel around the point, such that in the end positions thereof the moving panel is locked and the guide rail is aligned in a secure manner selectively with either the first route or with the second route.