Multiplex analysis method allowing the detection of a signal corresponding to the presence of an analyte to be improved, comprising the following steps: a) providing a solid support comprising at least one compartment, said compartment comprising at least two spots for the detection of an analyte, b) placing a sample to be analysed in the presence of the spots of said compartment, c) placing at least one detection ligand of an analyte in the presence of the spots of said compartment, said detection ligand of an analyte being coupled to a direct or indirect detection marker, d) when said detection marker is an indirect marker, placing a reporter of the indirect detection marker coupled to said detection ligand in the presence of the spots of said compartment, e) when the reporter used in step d) is coupled to an indirect marker, placing a reporter of the indirect detection marker coupled to said reporter in the presence of the spots of said compartment, f) placing absorbent particles in the presence of the spots of said compartment, said absorbent particles being contained in a liquid phase in contact with the spots of said compartment, said absorbent particles being carbon particles or coloured particles and said absorbent particles being supplied in the form of an absorbent composition comprising at least one compound selected from the group consisting of luminol, isoluminol, a derivative of luminol or isoluminol, an electron mediator and an oxidising agent, and g) detecting a signal corresponding to the presence of an analyte at the spots of said compartment, in the presence of the liquid phase comprising said absorbent particles, said signal being emitted by a luminescent compound and/or by a fluorophore, the method further comprising one or more washing steps permitting the removal of the compounds that are not bonded to the spots or to the various compounds bonded directly or indirectly to the spots, and no washing step being performed between step f) and g). a) providing a solid support comprising at least one compartment, said compartment comprising at least two spots for the detection of an analyte, b) placing a sample to be analysed in the presence of the spots of said compartment, c) placing at least one detection ligand of an analyte in the presence of the spots of said compartment, said detection ligand of an analyte being coupled to a direct or indirect detection marker, d) when said detection marker is an indirect marker, placing a reporter of the indirect detection marker coupled to said detection ligand in the presence of the spots of said compartment, e) when the reporter used in step d) is coupled to an indirect marker, placing a reporter of the indirect detection marker coupled to said reporter in the presence of the spots of said compartment, f) placing absorbent particles in the presence of the spots of said compartment, said absorbent particles being contained in a liquid phase in contact with the spots of said compartment, said absorbent particles being carbon particles or coloured particles and said absorbent particles being supplied in the form of an absorbent composition comprising at least one compound selected from the group consisting of luminol, isoluminol, a derivative of luminol or isoluminol, an electron mediator and an oxidising agent, and g) detecting a signal corresponding to the presence of an analyte at the spots of said compartment, in the presence of the liquid phase comprising said absorbent particles, said signal being emitted by a luminescent compound and/or by a fluorophore,