An immunoassay cartridge comprising: a reaction chamber in which a plurality of antibodies or antigens are attached to an inner surface including a bottom surface closest to a fluorescence sensor assembly; and a fluorescence sensor assembly disposed on a bottom surface of the reaction chamber, wherein the fluorescence sensor assembly comprises: an emission filter disposed below the reaction chamber, the emission filter having an optical characteristic capable of filtering an excitation light regardless of an incident angle of the excitation light and transmitting a radiation light having a wavelength larger than that of the excitation light; characterized in that the emission filter comprises: a base medium arranged in a flat shape and including a material which is transparent and which does not generate fluorescence or phosphorescence by excitation light; a semi-cured photoresist disposed in the base medium and fixed in a solid state by at least one method selected from the group consisting of thermosetting, photocuring and drying; and a pigment disposed in the base medium and absorbing light of a predetermined wavelength. a reaction chamber in which a plurality of antibodies or antigens are attached to an inner surface including a bottom surface closest to a fluorescence sensor assembly; and a fluorescence sensor assembly disposed on a bottom surface of the reaction chamber, wherein the fluorescence sensor assembly comprises: an emission filter disposed below the reaction chamber, the emission filter having an optical characteristic capable of filtering an excitation light regardless of an incident angle of the excitation light and transmitting a radiation light having a wavelength larger than that of the excitation light; characterized in that the emission filter comprises: a base medium arranged in a flat shape and including a material which is transparent and which does not generate fluorescence or phosphorescence by excitation light; a semi-cured photoresist disposed in the base medium and fixed in a solid state by at least one method selected from the group consisting of thermosetting, photocuring and drying; and a pigment disposed in the base medium and absorbing light of a predetermined wavelength. an emission filter disposed below the reaction chamber, the emission filter having an optical characteristic capable of filtering an excitation light regardless of an incident angle of the excitation light and transmitting a radiation light having a wavelength larger than that of the excitation light; characterized in that the emission filter comprises: a base medium arranged in a flat shape and including a material which is transparent and which does not generate fluorescence or phosphorescence by excitation light; a semi-cured photoresist disposed in the base medium and fixed in a solid state by at least one method selected from the group consisting of thermosetting, photocuring and drying; and a pigment disposed in the base medium and absorbing light of a predetermined wavelength. a base medium arranged in a flat shape and including a material which is transparent and which does not generate fluorescence or phosphorescence by excitation light; a semi-cured photoresist disposed in the base medium and fixed in a solid state by at least one method selected from the group consisting of thermosetting, photocuring and drying; and a pigment disposed in the base medium and absorbing light of a predetermined wavelength.