A method for the clonal pre-amplification of RNA molecules comprising the steps of a) providing a plurality of different RNA and preferably mRNA molecules, b1) hybridizing a first single stranded adaptor nucleic acid molecule to said plurality of different RNA molecules, said adaptor molecule comprising - a 5' terminal part representing a primer binding site, and - a 3' terminal part which is either an oligo dT sequence of at least 5 nucleotides in length or an essentially randomized sequence of at least 5 nucleotides in length, b2) performing a first strand cDNA synthesis in the presence of an RNA dependent DNA polymerase and a dNTP mixture in order to generate a pool of single stranded cDNAs, b3) attaching a second single stranded adaptor molecule to said pool of single stranded cDNAs, wherein step b3) comprises b3i) performing a terminal transferase reaction in the presence of one specific dNTP in order to create a homopolymer overhang, b3ii) hybridizing a second single stranded adaptor molecule to said pool of single stranded cDNAs, said second single stranded adaptor molecule further comprising - a 5' terminal part representing a primer binding site which is either identical or different to the 5' terminal part of said first single stranded adaptor molecule, - a 3' terminal part of homopolymeric nucleotide residues, which is complementary to said homopolymer overhang created in step b3i), c) preparing a water in oil emulsion characterized in that the majority of water droplets comprises one or more members of said plurality of different RNA molecules, d) clonally amplifying said plurality of different RNA molecules. a) providing a plurality of different RNA and preferably mRNA molecules, b1) hybridizing a first single stranded adaptor nucleic acid molecule to said plurality of different RNA molecules, said adaptor molecule comprising - a 5' terminal part representing a primer binding site, and - a 3' terminal part which is either an oligo dT sequence of at least 5 nucleotides in length or an essentially randomized sequence of at least 5 nucleotides in length, - a 5' terminal part representing a primer binding site, and - a 3' terminal part which is either an oligo dT sequence of at least 5 nucleotides in length or an essentially randomized sequence of at least 5 nucleotides in length, b2) performing a first strand cDNA synthesis in the presence of an RNA dependent DNA polymerase and a dNTP mixture in order to generate a pool of single stranded cDNAs, b3) attaching a second single stranded adaptor molecule to said pool of single stranded cDNAs, wherein step b3) comprises b3i) performing a terminal transferase reaction in the presence of one specific dNTP in order to create a homopolymer overhang, b3ii) hybridizing a second single stranded adaptor molecule to said pool of single stranded cDNAs, said second single stranded adaptor molecule further comprising - a 5' terminal part representing a primer binding site which is either identical or different to the 5' terminal part of said first single stranded adaptor molecule, - a 3' terminal part of homopolymeric nucleotide residues, which is complementary to said homopolymer overhang created in step b3i), - a 5' terminal part representing a primer binding site which is either identical or different to the 5' terminal part of said first single stranded adaptor molecule, - a 3' terminal part of homopolymeric nucleotide residues, which is complementary to said homopolymer overhang created in step b3i), c) preparing a water in oil emulsion characterized in that the majority of water droplets comprises one or more members of said plurality of different RNA molecules, d) clonally amplifying said plurality of different RNA molecules.