mhurhangee/ep-ind-claims huggingface dataset:
A method for tracking nucleic acid target origin by barcode tagging comprising: a. providing, in an open bulk reaction without partition, a plurality of microparticles, wherein some microparticles each have a clonal barcode template or a semi clonal barcode template immobilized thereon, wherein each barcode template comprises a barcode sequence and at least one flanking handle sequence; wherein other microparticles do not have barcode templates and serve as spacer in downstream reactions; b. providing a transposable DNA and a transposase; c. contacting a nucleic acid target with said transposase and said transposable DNA to form a transpososome-nucleic acid complex; d. attaching the nucleic acid target within said transpososome-nucleic acid complex to a barcode template on a microparticle; wherein the attached transpososome-nucleic acid complex is not divided from a second transpososome-nucleic acid complex by a partition; and e. breaking said nucleic acid target into fragments by removing said transposase from said transpososome-nucleic acid complex, wherein each fragment of the target nucleic acid target is attached to a barcode template, thereby forming barcode tagged fragments. a. providing, in an open bulk reaction without partition, a plurality of microparticles, wherein some microparticles each have a clonal barcode template or a semi clonal barcode template immobilized thereon, wherein each barcode template comprises a barcode sequence and at least one flanking handle sequence; wherein other microparticles do not have barcode templates and serve as spacer in downstream reactions; b. providing a transposable DNA and a transposase; c. contacting a nucleic acid target with said transposase and said transposable DNA to form a transpososome-nucleic acid complex; d. attaching the nucleic acid target within said transpososome-nucleic acid complex to a barcode template on a microparticle; wherein the attached transpososome-nucleic acid complex is not divided from a second transpososome-nucleic acid complex by a partition; and e. breaking said nucleic acid target into fragments by removing said transposase from said transpososome-nucleic acid complex, wherein each fragment of the target nucleic acid target is attached to a barcode template, thereby forming barcode tagged fragments.
Separation device, in particular an intelligent rubbish bin, for separating and collecting objects that can be used several times and residual waste, comprising: a first collection container for collecting residual waste; a second collection container; a sorting device; characterised in that: the second collection container is intended for receiving marked objects; the sorting device comprises at least one RFID scanner and is designed to recognise objects marked with an RFID identifier by means of the RFID scanner; and the sorting device is further designed to transfer recognised objects into the second collection container and to transfer other objects into the first collection container. a first collection container for collecting residual waste; a second collection container; a sorting device; the second collection container is intended for receiving marked objects; the sorting device comprises at least one RFID scanner and is designed to recognise objects marked with an RFID identifier by means of the RFID scanner; and the sorting device is further designed to transfer recognised objects into the second collection container and to transfer other objects into the first collection container.
Probe system comprising a probe head and a transceiver element, wherein the probe head has a stylus (1.2) and a sensor element (1.5) and a sensor signal can be generated by the sensor element (1.5) as a result of contact of the stylus (1.2), and the probe head and the transceiver element are configured in such a manner that an item of information (AI, BI) can be wirelessly transmitted between them via a radio signal, characterized in that the transceiver element or the probe head has two antennas (2.1, 2.2) arranged at a distance from one another in order to receive the radio signal and is configured in such a manner that the information (AI, BI) received by the two antennas (2.1, 2.2) via the radio signal can be processed in a parallel manner, wherein a first level of the radio signal received by the first antenna (2.1) can be determined, and a second level of the radio signal received by the second antenna (2.2) can be determined, and the probe system has a comparator (2.5) which can be used to compare the first level with the second level, and radio signals can be alternately transmitted by the two antennas (2.1, 2.2), with the result that the radio signal can be transmitted by only one antenna (2.1), while the other antenna (2.2) is silent, wherein the transmitting antenna (2.1) is that antenna (2.1) which provides the higher determined level from the comparison which was previously carried out.
An arm for a construction machine formed as a box-shaped structural body having a square cross sectional surface having left and right side plates (12, 13), an upper plate joined to the upper end sides of said left and right side plates (12, 13) by welding, a lower plate joined to the lower end sides of said left and right side plates (12, 13) by welding, and a rear plate joined to the rear end sides of said left and right side plates (12, 13) and to the rear end side of said upper plate by welding, comprising: a boom connecting boss located on the rear parts on the lower sides of said left and right side plates (12, 13) and joined to said left and right side plates (12, 13) and the rear end of said lower plate and the front end of said rear plate by welding; a bucket connecting boss joined to the front ends of said left and right side plates (12, 13), said upper plate, and said lower plate by welding; a pair of left and right arm cylinder brackets joined to an outer surface of said rear plate by welding; a pair of left and right bucket cylinder brackets joined to an outer surface (14A3) on the rear end side of said upper plate by welding; and said upper plate has a portion located on the rear side of said bucket cylinder brackets (23_bent diagonally downward, characterized in that: an upper end of said rear plate is provided by protruding upward from a height position of an outer surface (14A3) of said upper plate and the portion located on the rear side of said bucket cylinder brackets bent diagonally downward; and upper ends of said pair of arm cylinder brackets are arranged higher than a height position of said outer surface (14A3) of said upper plate and the portion located on the rear side of said bucket cylinder bracket bent diagonally downward and at a position lower than said upper end of said rear plate. a boom connecting boss located on the rear parts on the lower sides of said left and right side plates (12, 13) and joined to said left and right side plates (12, 13) and the rear end of said lower plate and the front end of said rear plate by welding; a bucket connecting boss joined to the front ends of said left and right side plates (12, 13), said upper plate, and said lower plate by welding; a pair of left and right arm cylinder brackets joined to an outer surface of said rear plate by welding; a pair of left and right bucket cylinder brackets joined to an outer surface (14A3) on the rear end side of said upper plate by welding; and said upper plate has a portion located on the rear side of said bucket cylinder brackets (23_bent diagonally downward, characterized in that: an upper end of said rear plate is provided by protruding upward from a height position of an outer surface (14A3) of said upper plate and the portion located on the rear side of said bucket cylinder brackets bent diagonally downward; and upper ends of said pair of arm cylinder brackets are arranged higher than a height position of said outer surface (14A3) of said upper plate and the portion located on the rear side of said bucket cylinder bracket bent diagonally downward and at a position lower than said upper end of said rear plate.
A lubricating composition, suitable for use as an automotive engine oil, comprising: a) an amide which is the reaction product of a secondary, branched amine and a carboxylic acid; and b) at least one additive and c) a further base oil chosen from any of the Group I to Group V base oils or a mixture thereof wherein the amide a) is an amide of Formula (Ia) or (Ib): wherein: R 1 and R 2 are independently selected from the group consisting of C 3 to C 18 linear or branched, saturated or unsaturated, hydrocarbyl groups; R 3 is selected from the group consisting of C 3 to C 50 linear or branched, saturated or unsaturated hydrocarbyl groups; R 4 is selected from the group consisting of C 1 to C 50 linear or branched, saturated or unsaturated hydrocarbylene groups; and n is 0 or 1, wherein at least one of R 1 and R 2 is branched, wherein the lubricant composition comprises at least 1 wt% and up to 20 wt% of the amide, based on the total weight of the composition. a) an amide which is the reaction product of a secondary, branched amine and a carboxylic acid; and b) at least one additive and c) a further base oil chosen from any of the Group I to Group V base oils or a mixture thereof wherein the amide a) is an amide of Formula (Ia) or (Ib): wherein: R 1 and R 2 are independently selected from the group consisting of C 3 to C 18 linear or branched, saturated or unsaturated, hydrocarbyl groups; R 3 is selected from the group consisting of C 3 to C 50 linear or branched, saturated or unsaturated hydrocarbyl groups; R 4 is selected from the group consisting of C 1 to C 50 linear or branched, saturated or unsaturated hydrocarbylene groups; and n is 0 or 1, R 1 and R 2 are independently selected from the group consisting of C 3 to C 18 linear or branched, saturated or unsaturated, hydrocarbyl groups; R 3 is selected from the group consisting of C 3 to C 50 linear or branched, saturated or unsaturated hydrocarbyl groups; R 4 is selected from the group consisting of C 1 to C 50 linear or branched, saturated or unsaturated hydrocarbylene groups; and n is 0 or 1, wherein at least one of R 1 and R 2 is branched, wherein the lubricant composition comprises at least 1 wt% and up to 20 wt% of the amide, based on the total weight of the composition.
A transaminase enzyme, comprising: a polypeptide having transaminase activity with increased thermostability at 50°C as compared to the polypeptide of SEQ ID NO:2, wherein the polypeptide comprises an amino acid sequence that is at least 80% identical to SEQ ID NO:18 and has as compared to SEQ ID NO:2 the set of substitutions: X9 is T, X45 is H, X86 is Y, X177 is L, X211 is K, X294 is V, X324 is G, and X391 is A, in which the polypeptide has at least about 10% residual activity in the conversion of pyruvate to L-alanine in presence of amino donor isopropylamine after treatment of the polypeptide at 50°C for 23 h. a polypeptide having transaminase activity with increased thermostability at 50°C as compared to the polypeptide of SEQ ID NO:2, wherein the polypeptide comprises an amino acid sequence that is at least 80% identical to SEQ ID NO:18 and has as compared to SEQ ID NO:2 the set of substitutions: X9 is T, X45 is H, X86 is Y, X177 is L, X211 is K, X294 is V, X324 is G, and X391 is A, in which the polypeptide has at least about 10% residual activity in the conversion of pyruvate to L-alanine in presence of amino donor isopropylamine after treatment of the polypeptide at 50°C for 23 h.
Dashboard for motor vehicles, comprising - a dial, - a light box, - a needle, and - a printed circuit board, the needle being driven in rotation, by a motor element, behind the dial and about an axis of rotation (2.5), the needle comprising a first axial portion (2.2), a second axial portion (2.3), a radial portion (2.4), and a display portion (2.1), the display portion (2.1) being visible at the edge of the dial, the dial comprising a first clearance (7.1) through which the needle is threaded, the light box comprising a second clearance (7.2) through which the needle is threaded, the dashboard comprising a display surface on one side of the printed circuit board and the motor element being fixed to the printed circuit board on the other side of the printed circuit board, and the printed circuit board comprising a third clearance (7.3) through which the needle is threaded, characterised in that the first, second and third clearances (7.1, 7.2, 7.3) are of an elongate curved shape, which is coaxial with the axis of rotation, and are located at a distance from the axis of rotation (2.5) which corresponds to the length of the radial portion (2.4) of the needle. - a dial, - a light box, - a needle, and - a printed circuit board,
A laser parameter system for optimization of spherical aberration parameters for corneal laser treatments, the system comprising: a processor having access to memory media storing instructions executable by the processor to: receive pupillometry data for a patient subject to a corneal laser treatment, the pupillometry data comprising respective pupil diameters including: a mesopic diameter and a photopic diameter; calculate an outer diameter for the spherical aberration that is greater than the mesopic diameter; calculate an inner diameter for the spherical aberration associated with an amplitude of the spherical aberration that corresponds to the photopic diameter; and use the spherical aberration calculated, including the outer diameter and the inner diameter, to cause the corneal laser treatment to be performed on the patient, characterized in that the pupil diameters further include a normal light diameter and an accommodative reading diameter; and that the instructions are executable by the processor to: calculate the amplitude of the spherical aberration according to a refraction that induces myopia; calculate a slope of the spherical aberration extending from the photopic diameter to the normal light diameter; and use the spherical aberration calculated, including the amplitude and the slope, to cause the corneal laser treatment to be performed on the patient. receive pupillometry data for a patient subject to a corneal laser treatment, the pupillometry data comprising respective pupil diameters including: a mesopic diameter and a photopic diameter; calculate an outer diameter for the spherical aberration that is greater than the mesopic diameter; calculate an inner diameter for the spherical aberration associated with an amplitude of the spherical aberration that corresponds to the photopic diameter; and use the spherical aberration calculated, including the outer diameter and the inner diameter, to cause the corneal laser treatment to be performed on the patient, the pupil diameters further include a normal light diameter and an accommodative reading diameter; and that the instructions are executable by the processor to: calculate the amplitude of the spherical aberration according to a refraction that induces myopia; calculate a slope of the spherical aberration extending from the photopic diameter to the normal light diameter; and use the spherical aberration calculated, including the amplitude and the slope, to cause the corneal laser treatment to be performed on the patient. calculate the amplitude of the spherical aberration according to a refraction that induces myopia; calculate a slope of the spherical aberration extending from the photopic diameter to the normal light diameter; and use the spherical aberration calculated, including the amplitude and the slope, to cause the corneal laser treatment to be performed on the patient.
A method for producing a rare earth sintered magnet comprising the steps of depositing a coating of R-containing particles on at least a portion of the surface of a rare earth magnet body, and heat treating the magnet body for causing absorption and diffusion of R in the magnet body, R being at least one element selected from rare earth elements, wherein the depositing step relies on an aerosol deposition method, and the deposited coating is a porous coating and has a density which is 40 to 70% of the true density of the R-containing particles.
Semi-submersible platform, characterized in that it comprises: - at least two longitudinal caissons having solid lateral walls (3, 4) delimiting a central channel which extends from a bow end to a stern end of the platform; - at least one beam transversely coupling the caissons; - at least one stabilizing fin which extends transversely below the lower edges of the caissons. - at least two longitudinal caissons having solid lateral walls (3, 4) delimiting a central channel which extends from a bow end to a stern end of the platform; - at least one beam transversely coupling the caissons; - at least one stabilizing fin which extends transversely below the lower edges of the caissons.
A method for distributing components (10a, 10b, 10c, 10d) into material kit carriers, comprising: supplying, at a feed station, at least two components (10a, 10b, 10c, 10d), performing, at the feed station, at least one machining operation on at least one of said components (10a, 10b, 10c, 10d), and arranging said components (10a, 10b, 10c, 10d) in at least one material kit carrier located at a distance from the feed station, such that each of said material kit carriers contains a plurality of said non-assembled components (10a, 10b, 10c, 10d) which are adapted to be joined together to form part of a predetermined product, arranging, at the feed station, at least two of said components on a common intermediate carrier (5a, 5b), moving the intermediate carrier (5a, 5b) to said material kit carrier, and transferring at least one of said components (10a, 10b, 10c, 10d) from the intermediate carrier (5a, 5b) into said material kit carrier while the material kit carrier is located in a storage rack. supplying, at a feed station, at least two components (10a, 10b, 10c, 10d), performing, at the feed station, at least one machining operation on at least one of said components (10a, 10b, 10c, 10d), and arranging said components (10a, 10b, 10c, 10d) in at least one material kit carrier located at a distance from the feed station, such that each of said material kit carriers contains a plurality of said non-assembled components (10a, 10b, 10c, 10d) which are adapted to be joined together to form part of a predetermined product, arranging, at the feed station, at least two of said components on a common intermediate carrier (5a, 5b), moving the intermediate carrier (5a, 5b) to said material kit carrier, and transferring at least one of said components (10a, 10b, 10c, 10d) from the intermediate carrier (5a, 5b) into said material kit carrier while the material kit carrier is located in a storage rack.
A liquid crystal display, comprising: a first substrate having a top surface and a bottom surface; a pixel electrode formed on the top surface of the first substrate and divided into a plurality of portions, each portion being oval-shaped, the oval-shaped portions being sequentially interconnected in a longitudinal direction of the pixel electrode; a second substrate having a top surface and a bottom surface; a common electrode formed on the bottom surface of the second substrate; and a liquid crystal layer sandwiched between the top surface of the first substrate and the bottom surface of the second substrate, the common electrode comprising a first diamond shaped opening pattern being placed at the center of the corresponding oval shaped portion of the pixel electrode, the common electrode further comprising left and right openings (380, 390) surrounding the diamond-shaped openings and surrounding the pixel electrode at a predetermined distance. a first substrate having a top surface and a bottom surface; a pixel electrode formed on the top surface of the first substrate and divided into a plurality of portions, each portion being oval-shaped, the oval-shaped portions being sequentially interconnected in a longitudinal direction of the pixel electrode; a second substrate having a top surface and a bottom surface; a common electrode formed on the bottom surface of the second substrate; and a liquid crystal layer sandwiched between the top surface of the first substrate and the bottom surface of the second substrate, the common electrode comprising a first diamond shaped opening pattern being placed at the center of the corresponding oval shaped portion of the pixel electrode, the common electrode further comprising left and right openings (380, 390) surrounding the diamond-shaped openings and surrounding the pixel electrode at a predetermined distance.
A method for producing a high purity isocyanatoorganosilane of the general formula R 2 R 3 R 4 Si-R 1 -N=C=O, comprising: feeding a carbamatoorganosilane of the general formula R 2 R 3 R 4 Si-R 1 -NH-CO-OR, where R is a monovalent alkyl radical containing from 1 to 10 carbon atoms or an aromatic group of from 6 to 12 carbon atoms, R 1 is a divalent hydrocarbon radical containing from 1 to 6 carbon atoms, and R 2, R 3 and R 4 are independently selected from the group consisting of methyl, ethyl, n-propyl, isopropyl, methoxy, ethoxy, n-propoxy and isopropoxy radicals, to a cracking device; heating the fed carbamatoorganosilane to a temperature and pressure effective to thermally dissociate the carbamatoorganosilane to a reaction product mixture comprising isocyanatoorganosilane, alcohol, unreacted carbamatoorganosilane, and heavies, separating isocyanatoorganosilane having a purity greater than 90% from the rest of the reaction product mixture in a distillation column having a bottom part and a top part, and collecting the isocyanatoorganosilane having greater than 90% purity through a side stream which is located between the bottom part of the distillation column and the top part of the distillation column and wherein the ratio of the length of the bottom part of the distillation column to the length of the top part of the distillation column is from 2:1 to 10:1. feeding a carbamatoorganosilane of the general formula R 2 R 3 R 4 Si-R 1 -NH-CO-OR, where R is a monovalent alkyl radical containing from 1 to 10 carbon atoms or an aromatic group of from 6 to 12 carbon atoms, R 1 is a divalent hydrocarbon radical containing from 1 to 6 carbon atoms, and R 2, R 3 and R 4 are independently selected from the group consisting of methyl, ethyl, n-propyl, isopropyl, methoxy, ethoxy, n-propoxy and isopropoxy radicals, to a cracking device; R is a monovalent alkyl radical containing from 1 to 10 carbon atoms or an aromatic group of from 6 to 12 carbon atoms, R 1 is a divalent hydrocarbon radical containing from 1 to 6 carbon atoms, and R 2, R 3 and R 4 are independently selected from the group consisting of methyl, ethyl, n-propyl, isopropyl, methoxy, ethoxy, n-propoxy and isopropoxy radicals, to a cracking device; heating the fed carbamatoorganosilane to a temperature and pressure effective to thermally dissociate the carbamatoorganosilane to a reaction product mixture comprising isocyanatoorganosilane, alcohol, unreacted carbamatoorganosilane, and heavies, separating isocyanatoorganosilane having a purity greater than 90% from the rest of the reaction product mixture in a distillation column having a bottom part and a top part, and collecting the isocyanatoorganosilane having greater than 90% purity through a side stream which is located between the bottom part of the distillation column and the top part of the distillation column and