A display substrate comprising: a base substrate; a plurality of pixel units, each of the plurality of the pixel units comprising a first electrode and a second electrode on the base substrate, and at least one of the pixel units further comprising an auxiliary electrode and a connecting structure between the auxiliary electrode and the first electrode, with an orthogonal projection of the auxiliary electrode on the base substrate being at least partially contained within an orthogonal projection of the first electrode on the base substrate, and the connecting structure being configured to electrically connect the auxiliary electrode to the first electrode, a connecting structure electrically connecting the auxiliary electrode to the first electrode, wherein the at least one of the pixel units comprises an isolation layer between the first electrode and the auxiliary electrode, the first electrode and the auxiliary electrode overlapping the isolation layer, wherein the isolation layer comprises a first part, which has a thickness smaller than the thickness of the other parts of the isolation layer such that the breakdown voltage of the first part is lower than a first voltage difference existing between voltages applied on the auxiliary electrode and the first electrode respectively the connecting structure having been formed by the first part of the isolation layer having been subject to electrical breakdown under the action of a voltage of a first voltage difference on the auxiliary electrode and on the first electrode, respectively, such that after the electrical breakdown the first part of the isolation layer located between the auxiliary electrode and the first electrode becomes conductive, so that the first part electrically connects the auxiliary electrode with the first electrode, wherein the at least one of the pixel units further comprises an organic light-emitting diode comprising the first electrode, a light emitting layer and the second electrode, and the isolation layer and at least a part of the light-emitting layer of the organic light-emitting diode are made from a same thin film with the same material. a base substrate; a plurality of pixel units, each of the plurality of the pixel units comprising a first electrode and a second electrode on the base substrate, and at least one of the pixel units further comprising an auxiliary electrode and a connecting structure between the auxiliary electrode and the first electrode, with an orthogonal projection of the auxiliary electrode on the base substrate being at least partially contained within an orthogonal projection of the first electrode on the base substrate, and the connecting structure being configured to electrically connect the auxiliary electrode to the first electrode, a connecting structure electrically connecting the auxiliary electrode to the first electrode, wherein the at least one of the pixel units comprises an isolation layer between the first electrode and the auxiliary electrode, the first electrode and the auxiliary electrode overlapping the isolation layer, wherein the isolation layer comprises a first part, which has a thickness smaller than the thickness of the other parts of the isolation layer such that the breakdown voltage of the first part is lower than a first voltage difference existing between voltages applied on the auxiliary electrode and the first electrode respectively the connecting structure having been formed by the first part of the isolation layer having been subject to electrical breakdown under the action of a voltage of a first voltage difference on the auxiliary electrode and on the first electrode, respectively, such that after the electrical breakdown the first part of the isolation layer located between the auxiliary electrode and the first electrode becomes conductive, so that the first part electrically connects the auxiliary electrode with the first electrode, wherein the at least one of the pixel units further comprises an organic light-emitting diode comprising the first electrode, a light emitting layer and the second electrode, and the isolation layer and at least a part of the light-emitting layer of the organic light-emitting diode are made from a same thin film with the same material.