A display substrate, comprising: a substrate, a plurality of pixels, and a pixel definition layer on the substrate, wherein: the plurality of pixels comprise long sides and short sides; the pixel definition layer comprises long-side sections adjacent to the long sides, and short-side sections adjacent to the short sides, and heights of the long-side sections are greater than heights of the short-side sections, characterised in that: the pixel definition layer comprises a lyophilic material layer, and a lyophobic material layer on a side of the lyophilic material layer away from the substrate; a thickness s1 of the lyophobic material layer, and a thickness s2 of the lyophilic material layer of a long-side section satisfy s1≥1/2 ∗ s2; a length a of at least one of the long sides, a length b of at least one of the short sides, a height h1 of at least one of the long-side sections, and a height h2 of at least one of the short-side sections satisfy h1/h2 = (1/3∼3/4)a/b; a height h1 of at least one of the long-side sections ranges from 1μm to 5μm, and a height h2 of at least one of the short-side sections ranges from 0.5μm to 2.5μm; wall sides of the pixel definition layer facing the plurality of pixels are slope faces; and each of the slope faces are at a slope angle to the substrate; a slope angle of the slope faces facing the long sides of the plurality of pixels is equal to a slope angle of the slope faces facing the short sides of the plurality of pixels. a substrate, a plurality of pixels, and a pixel definition layer on the substrate, wherein: the plurality of pixels comprise long sides and short sides; the pixel definition layer comprises long-side sections adjacent to the long sides, and short-side sections adjacent to the short sides, and heights of the long-side sections are greater than heights of the short-side sections, characterised in that: the pixel definition layer comprises a lyophilic material layer, and a lyophobic material layer on a side of the lyophilic material layer away from the substrate; a thickness s1 of the lyophobic material layer, and a thickness s2 of the lyophilic material layer of a long-side section satisfy s1≥1/2 ∗ s2; a length a of at least one of the long sides, a length b of at least one of the short sides, a height h1 of at least one of the long-side sections, and a height h2 of at least one of the short-side sections satisfy h1/h2 = (1/3∼3/4)a/b; a height h1 of at least one of the long-side sections ranges from 1μm to 5μm, and a height h2 of at least one of the short-side sections ranges from 0.5μm to 2.5μm; wall sides of the pixel definition layer facing the plurality of pixels are slope faces; and each of the slope faces are at a slope angle to the substrate; a slope angle of the slope faces facing the long sides of the plurality of pixels is equal to a slope angle of the slope faces facing the short sides of the plurality of pixels. the plurality of pixels comprise long sides and short sides; the pixel definition layer comprises long-side sections adjacent to the long sides, and short-side sections adjacent to the short sides, and heights of the long-side sections are greater than heights of the short-side sections, characterised in that: the pixel definition layer comprises a lyophilic material layer, and a lyophobic material layer on a side of the lyophilic material layer away from the substrate; a thickness s1 of the lyophobic material layer, and a thickness s2 of the lyophilic material layer of a long-side section satisfy s1≥1/2 ∗ s2; a length a of at least one of the long sides, a length b of at least one of the short sides, a height h1 of at least one of the long-side sections, and a height h2 of at least one of the short-side sections satisfy h1/h2 = (1/3∼3/4)a/b; a height h1 of at least one of the long-side sections ranges from 1μm to 5μm, and a height h2 of at least one of the short-side sections ranges from 0.5μm to 2.5μm; wall sides of the pixel definition layer facing the plurality of pixels are slope faces; and each of the slope faces are at a slope angle to the substrate; a slope angle of the slope faces facing the long sides of the plurality of pixels is equal to a slope angle of the slope faces facing the short sides of the plurality of pixels. the pixel definition layer comprises a lyophilic material layer, and a lyophobic material layer on a side of the lyophilic material layer away from the substrate; a thickness s1 of the lyophobic material layer, and a thickness s2 of the lyophilic material layer of a long-side section satisfy s1≥1/2 ∗ s2; a length a of at least one of the long sides, a length b of at least one of the short sides, a height h1 of at least one of the long-side sections, and a height h2 of at least one of the short-side sections satisfy h1/h2 = (1/3∼3/4)a/b; a height h1 of at least one of the long-side sections ranges from 1μm to 5μm, and a height h2 of at least one of the short-side sections ranges from 0.5μm to 2.5μm; wall sides of the pixel definition layer facing the plurality of pixels are slope faces; and each of the slope faces are at a slope angle to the substrate; a slope angle of the slope faces facing the long sides of the plurality of pixels is equal to a slope angle of the slope faces facing the short sides of the plurality of pixels.