A primer probe combination for detecting separate Candida types, comprising specific primers, detection probes and complementary probes specified at Candida albicans, Candida tropicalis, Candida parapsilosis, Candida krusei and Candida glabrata respectively, wherein the complementary probes and the detection probes are in incomplete complementary pairing; wherein nucleotide sequences of the specific primers of the Candida albicans are shown as SEQ ID NO. 1-2; nucleotide sequences of the specific primers of the Candida tropicalis are shown as SEQ ID NO.3-4; nucleotide sequences of the specific primers of the Candida parapsilosis are shown as SEQ ID NO. 5-6; nucleotide sequences of the specific primers of the Candida krusei are shown as SEQ ID NO. 7-8; and nucleotide sequences of the specific primers of the Candida glabrata are shown as SEQ ID NO. 9-10; a nucleotide sequence of the detection probe of the Candida albicans is shown as SEQ ID NO. 11; a nucleotide sequence of the detection probe of the Candida tropicalis is shown as SEQ ID NO. 12; a nucleotide sequence of the detection probe of the Candida parapsilosis is shown as SEQ ID NO. 13; a nucleotide sequence of the detection probe of the Candida krusei is shown as SEQ ID NO. 14; and a nucleotide sequence of the detection probe of the Candida glabrata is shown as SEQ ID NO. 15; a nucleotide sequence of the complementary probe of the Candida albicans is shown as SEQ ID NO. 16; a nucleotide sequence of the complementary probe of the Candida tropicalis is shown as SEQ ID NO. 17; a nucleotide sequence of the complementary probe of the Candida parapsilosis is shown as SEQ ID NO. 18; a nucleotide sequence of the complementary probe of the Candida krusei is shown as SEQ ID NO. 19; and a nucleotide sequence of the complementary probe of the Candida glabrata is shown as SEQ ID NO. 20; wherein a quencher is modified at 3' end of the detection probe of each one of the Candida albicans, Candida tropicalis, Candida parapsilosis, Candida krusei and Candida glabrata; and a fluorophore is modified at 5' end of the detection probe of each one of the Candida albicans, Candida tropicalis, Candida parapsilosis, Candida krusei and Candida glabrata; the fluorophores modified at the 5' ends of the detection probes are the same. wherein nucleotide sequences of the specific primers of the Candida albicans are shown as SEQ ID NO. 1-2; nucleotide sequences of the specific primers of the Candida tropicalis are shown as SEQ ID NO.3-4; nucleotide sequences of the specific primers of the Candida parapsilosis are shown as SEQ ID NO. 5-6; nucleotide sequences of the specific primers of the Candida krusei are shown as SEQ ID NO. 7-8; and nucleotide sequences of the specific primers of the Candida glabrata are shown as SEQ ID NO. 9-10; a nucleotide sequence of the detection probe of the Candida albicans is shown as SEQ ID NO. 11; a nucleotide sequence of the detection probe of the Candida tropicalis is shown as SEQ ID NO. 12; a nucleotide sequence of the detection probe of the Candida parapsilosis is shown as SEQ ID NO. 13; a nucleotide sequence of the detection probe of the Candida krusei is shown as SEQ ID NO. 14; and a nucleotide sequence of the detection probe of the Candida glabrata is shown as SEQ ID NO. 15; a nucleotide sequence of the complementary probe of the Candida albicans is shown as SEQ ID NO. 16; a nucleotide sequence of the complementary probe of the Candida tropicalis is shown as SEQ ID NO. 17; a nucleotide sequence of the complementary probe of the Candida parapsilosis is shown as SEQ ID NO. 18; a nucleotide sequence of the complementary probe of the Candida krusei is shown as SEQ ID NO. 19; and a nucleotide sequence of the complementary probe of the Candida glabrata is shown as SEQ ID NO. 20; wherein a quencher is modified at 3' end of the detection probe of each one of the Candida albicans, Candida tropicalis, Candida parapsilosis, Candida krusei and Candida glabrata; and a fluorophore is modified at 5' end of the detection probe of each one of the Candida albicans, Candida tropicalis, Candida parapsilosis, Candida krusei and Candida glabrata; the fluorophores modified at the 5' ends of the detection probes are the same.