A method for processing an audio signal, comprising: acquiring an original noisy signal of each of at least two microphones by acquiring, using the at least two microphones, an audio signal emitted by each of at least two sound sources; for each frame in time domain, acquiring a frequency-domain estimated signal of each of the at least two sound sources according to the original noisy signal of each of the at least two microphones; determining a frequency collection containing a plurality of predetermined static frequencies and dynamic frequencies in a predetermined frequency band range, the dynamic frequencies being frequencies whose frequency data meeting a filter condition; determining a weighting coefficient of each frequency contained in the frequency collection according to the frequency-domain estimated signal of the each frequency in the frequency collection; determining a separation matrix of the each frequency according to the weighting coefficient; and acquiring, based on the separation matrix and the original noisy signal, the audio signal emitted by each of the at least two sound sources, characterized in that determining the frequency collection containing the plurality of the predetermined static frequencies and the dynamic frequencies in the predetermined frequency band range comprises: determining a plurality of harmonic subsets in the predetermined frequency band range, each of the harmonic subsets containing a plurality of frequency data, frequencies contained in the plurality of the harmonic subsets being the predetermined static frequencies; determining a dynamic frequency collection according to a condition number of an a priori separation matrix of the each frequency in the predetermined frequency band range, the a priori separation matrix comprising: a predetermined initial separation matrix or a separation matrix of the each frequency in a last frame; and determining the frequency collection according to a union of the harmonic subsets and the dynamic frequency collection. acquiring an original noisy signal of each of at least two microphones by acquiring, using the at least two microphones, an audio signal emitted by each of at least two sound sources; for each frame in time domain, acquiring a frequency-domain estimated signal of each of the at least two sound sources according to the original noisy signal of each of the at least two microphones; determining a frequency collection containing a plurality of predetermined static frequencies and dynamic frequencies in a predetermined frequency band range, the dynamic frequencies being frequencies whose frequency data meeting a filter condition; determining a weighting coefficient of each frequency contained in the frequency collection according to the frequency-domain estimated signal of the each frequency in the frequency collection; determining a separation matrix of the each frequency according to the weighting coefficient; and acquiring, based on the separation matrix and the original noisy signal, the audio signal emitted by each of the at least two sound sources, characterized in that determining the frequency collection containing the plurality of the predetermined static frequencies and the dynamic frequencies in the predetermined frequency band range comprises: determining a plurality of harmonic subsets in the predetermined frequency band range, each of the harmonic subsets containing a plurality of frequency data, frequencies contained in the plurality of the harmonic subsets being the predetermined static frequencies; determining a dynamic frequency collection according to a condition number of an a priori separation matrix of the each frequency in the predetermined frequency band range, the a priori separation matrix comprising: a predetermined initial separation matrix or a separation matrix of the each frequency in a last frame; and determining the frequency collection according to a union of the harmonic subsets and the dynamic frequency collection. determining a plurality of harmonic subsets in the predetermined frequency band range, each of the harmonic subsets containing a plurality of frequency data, frequencies contained in the plurality of the harmonic subsets being the predetermined static frequencies; determining a dynamic frequency collection according to a condition number of an a priori separation matrix of the each frequency in the predetermined frequency band range, the a priori separation matrix comprising: a predetermined initial separation matrix or a separation matrix of the each frequency in a last frame; and determining the frequency collection according to a union of the harmonic subsets and the dynamic frequency collection.