A composition of two or more engineered nucleic acid sequences forming a scaffold ("NASC"), the NASC composition comprising: a first engineered nucleic acid component ("NASC-PC1") comprising, in a 5' to 3' direction, a spacer element 1 comprising a nucleic acid target binding sequence 1, a repeat element 1 comprising a repeat nucleic acid sequence 1, and a nucleic acid binding protein binding element 1 comprising a double-stranded nucleic acid binding protein binding sequence 1, wherein the spacer element 1 is covalently connected with the repeat element 1, and the repeat element 1 is covalently connected with the nucleic acid binding protein binding element 1; and a second engineered nucleic acid component ("NASC-PC2") comprising, in a 5' to 3' direction, a spacer element 2 comprising a nucleic acid target binding sequence 2, a repeat element 2 comprising a repeat nucleic acid sequence 1C, and a nucleic acid binding protein binding element 2 comprising a double-stranded nucleic acid binding protein binding sequence 2, wherein the spacer element 2 is covalently connected with the repeat element 2, and the repeat element 2 is covalently connected with the nucleic acid binding protein binding element 2; wherein there is a connection between the repeat nucleic acid sequence 1 and the repeat nucleic acid sequence 1C through hydrogen-bonded base pairs, the connection forms the NASC composition, and the NASC composition is capable of binding a first Class 2 Type II CRISPR-Cas9 protein and a second Class 2 Type II CRISPR-Cas9 protein. a first engineered nucleic acid component ("NASC-PC1") comprising, in a 5' to 3' direction, a spacer element 1 comprising a nucleic acid target binding sequence 1, a repeat element 1 comprising a repeat nucleic acid sequence 1, and a nucleic acid binding protein binding element 1 comprising a double-stranded nucleic acid binding protein binding sequence 1, wherein the spacer element 1 is covalently connected with the repeat element 1, and the repeat element 1 is covalently connected with the nucleic acid binding protein binding element 1; and a spacer element 1 comprising a nucleic acid target binding sequence 1, a repeat element 1 comprising a repeat nucleic acid sequence 1, and a nucleic acid binding protein binding element 1 comprising a double-stranded nucleic acid binding protein binding sequence 1, wherein the spacer element 1 is covalently connected with the repeat element 1, and the repeat element 1 is covalently connected with the nucleic acid binding protein binding element 1; and a second engineered nucleic acid component ("NASC-PC2") comprising, in a 5' to 3' direction, a spacer element 2 comprising a nucleic acid target binding sequence 2, a repeat element 2 comprising a repeat nucleic acid sequence 1C, and a nucleic acid binding protein binding element 2 comprising a double-stranded nucleic acid binding protein binding sequence 2, wherein the spacer element 2 is covalently connected with the repeat element 2, and the repeat element 2 is covalently connected with the nucleic acid binding protein binding element 2; a spacer element 2 comprising a nucleic acid target binding sequence 2, a repeat element 2 comprising a repeat nucleic acid sequence 1C, and a nucleic acid binding protein binding element 2 comprising a double-stranded nucleic acid binding protein binding sequence 2, wherein the spacer element 2 is covalently connected with the repeat element 2, and the repeat element 2 is covalently connected with the nucleic acid binding protein binding element 2; wherein there is a connection between the repeat nucleic acid sequence 1 and the repeat nucleic acid sequence 1C through hydrogen-bonded base pairs, the connection forms the NASC composition, and the NASC composition is capable of binding a first Class 2 Type II CRISPR-Cas9 protein and a second Class 2 Type II CRISPR-Cas9 protein.