Method for nucleic acid sequencing comprising the following steps: (a) obtaining a plurality of genetic reads by sequencing of a nucleic acid sample; (b) aligning the plurality of genetic reads to at least one reference genetic sequence; (c) grouping the genetic reads sharing a genetic position on a reference genetic sequence of the at least one reference genetic sequence into a plurality of groups; (d) creating a consensus sequence for each group of the plurality of groups, wherein one corresponding consensus sequence is created by determining a most abundant nucleotide man_p at each specific position p of a plurality of positions within the one corresponding group of genetic reads and (i) setting a representation of the most abundant nucleotide man_p at each specific position p of the consensus sequence if a ratio r between the number of genetic reads within the one corresponding group having the most abundant nucleotide man_p at the specific position p and the number of genetic reads within the one corresponding group is above or equal a predetermined threshold t; and (ii) setting a tag N if the ratio r is below the predetermined threshold t, wherein said threshold t is at least about 76%; (e) comparing the consensus sequences of the plurality of groups to the reference genetic sequence at each specific position p of a plurality of positions of the consensus sequences, and wherein a difference at a specific position between the consensus sequences and the reference genetic sequence indicates a genetic variation at the specific position; (f) determining the number of consensus sequences comprising the variation at each specific position p of a plurality of positions, and determining the number of consensus sequences comprising the tag N at each specific position p of a plurality of positions; and (g) identifying the genetic variation at each specific position p of a plurality of positions as a true genetic variation if a ratio r* between the number of consensus sequences comprising the tag N at the specific position p and the number of the consensus sequences comprising the genetic variation at the specific position p is below a threshold t*, wherein said threshold t* is equal or below 1.8. (a) obtaining a plurality of genetic reads by sequencing of a nucleic acid sample; (b) aligning the plurality of genetic reads to at least one reference genetic sequence; (c) grouping the genetic reads sharing a genetic position on a reference genetic sequence of the at least one reference genetic sequence into a plurality of groups; (d) creating a consensus sequence for each group of the plurality of groups, wherein one corresponding consensus sequence is created by determining a most abundant nucleotide man_p at each specific position p of a plurality of positions within the one corresponding group of genetic reads and (i) setting a representation of the most abundant nucleotide man_p at each specific position p of the consensus sequence if a ratio r between the number of genetic reads within the one corresponding group having the most abundant nucleotide man_p at the specific position p and the number of genetic reads within the one corresponding group is above or equal a predetermined threshold t; and (ii) setting a tag N if the ratio r is below the predetermined threshold t, wherein said threshold t is at least about 76%; (i) setting a representation of the most abundant nucleotide man_p at each specific position p of the consensus sequence if a ratio r between the number of genetic reads within the one corresponding group having the most abundant nucleotide man_p at the specific position p and the number of genetic reads within the one corresponding group is above or equal a predetermined threshold t; and (ii) setting a tag N if the ratio r is below the predetermined threshold t, wherein said threshold t is at least about 76%; (e) comparing the consensus sequences of the plurality of groups to the reference genetic sequence at each specific position p of a plurality of positions of the consensus sequences, and wherein a difference at a specific position between the consensus sequences and the reference genetic sequence indicates a genetic variation at the specific position; (f) determining the number of consensus sequences comprising the variation at each specific position p of a plurality of positions, and determining the number of consensus sequences comprising the tag N at each specific position p of a plurality of positions; and (g) identifying the genetic variation at each specific position p of a plurality of positions as a true genetic variation if a ratio r* between the number of consensus sequences comprising the tag N at the specific position p and the number of the consensus sequences comprising the genetic variation at the specific position p is below a threshold t*, wherein said threshold t* is equal or below 1.8.