An electrophoresis cartridge (104, 207, 500) adapted to releasably engage with an electrophoresis cartridge interface of a system, wherein the electrophoresis cartridge (104, 207, 500) comprises: (a) an electrophoresis assembly including: an anode sub-assembly (407, 507) comprising an anode, a cathode sub-assembly (405, 510) comprising a cathode; and at least one electrophoresis capillary (406, 509) having a first end in fluidic communication with the cathode sub-assembly (405, 510) and a second end in fluidic communication with the anode sub-assembly (407, 507), wherein said cathode and said anode are configured to provide a voltage gradient across said first end and said second end of said at least one electrophoresis capillary (406, 509), (b) a plurality of containers (502, 503, 504, 505) comprising reagent; (c) at least one pump (520, 521, 522, 523) configured to transfer reagent from one or more container (502, 503, 504, 505) to the cathode sub-assembly (405, 510) through at least one respective fluid conduit, and at least one pump configured to transfer reagent from one or more container (502, 503, 504, 505) to the anode sub-assembly (407, 507) through at least one respective fluid conduit; and (d) a sample inlet port and at least one sample line, wherein the sample line places the sample inlet port in fluidic communication with the first end of the electrophoresis capillary (406, 509) through a passage in the cathode sub-assembly (405, 510) and the sample inlet port is configured to fluidically communicate with the sample cartridge interface (103, 519) of the system so as to direct a sample to the at least one electrophoresis capillary. (a) an electrophoresis assembly including: an anode sub-assembly (407, 507) comprising an anode, a cathode sub-assembly (405, 510) comprising a cathode; and at least one electrophoresis capillary (406, 509) having a first end in fluidic communication with the cathode sub-assembly (405, 510) and a second end in fluidic communication with the anode sub-assembly (407, 507), wherein said cathode and said anode are configured to provide a voltage gradient across said first end and said second end of said at least one electrophoresis capillary (406, 509), an anode sub-assembly (407, 507) comprising an anode, a cathode sub-assembly (405, 510) comprising a cathode; and at least one electrophoresis capillary (406, 509) having a first end in fluidic communication with the cathode sub-assembly (405, 510) and a second end in fluidic communication with the anode sub-assembly (407, 507), wherein said cathode and said anode are configured to provide a voltage gradient across said first end and said second end of said at least one electrophoresis capillary (406, 509), (b) a plurality of containers (502, 503, 504, 505) comprising reagent; (c) at least one pump (520, 521, 522, 523) configured to transfer reagent from one or more container (502, 503, 504, 505) to the cathode sub-assembly (405, 510) through at least one respective fluid conduit, and at least one pump configured to transfer reagent from one or more container (502, 503, 504, 505) to the anode sub-assembly (407, 507) through at least one respective fluid conduit; and (d) a sample inlet port and at least one sample line, wherein the sample line places the sample inlet port in fluidic communication with the first end of the electrophoresis capillary (406, 509) through a passage in the cathode sub-assembly (405, 510) and the sample inlet port is configured to fluidically communicate with the sample cartridge interface (103, 519) of the system so as to direct a sample to the at least one electrophoresis capillary.