A method for quantifying an analyte in a sample comprising: introducing the sample into at least one test split channel, wherein the test split channel comprises a test reaction portion, wherein the analyte in the sample is to bind to capture reagents provided in the test reaction portion; contacting the analyte bound to the capture reagents with a reactant solution, wherein the reactant solution comprises a plurality of reagent coated microparticles for binding with the analyte; receiving residual reactant solution, wherein the residual reactant solution comprises unbound microparticles; and analyzing the residual reactant solution to quantify the analyte; characterized in that the method comprises: introducing the sample at an inlet port of a first channel; distributing the sample between at least one test split channel and at least one control split channel from the first channel and binding of the analyte to the capture reagents provided in the reaction portion; washing the sample from the at least one test split channel and the at least one control split channel; introducing the reactant solution into the at least one test split channel and at least one control split channel; receiving by a test detection portion of the at least one test split channel and a control detection portion of the at least one control split channel residual reactant solution; determining a difference in volume of the test detection portion and the control detection portion; and correlating the difference in volume with the difference in initial number of reagent coated microparticles and unbound reagent coated microparticles. introducing the sample into at least one test split channel, wherein the test split channel comprises a test reaction portion, wherein the analyte in the sample is to bind to capture reagents provided in the test reaction portion; contacting the analyte bound to the capture reagents with a reactant solution, wherein the reactant solution comprises a plurality of reagent coated microparticles for binding with the analyte; receiving residual reactant solution, wherein the residual reactant solution comprises unbound microparticles; and analyzing the residual reactant solution to quantify the analyte; characterized in that the method comprises: introducing the sample at an inlet port of a first channel; distributing the sample between at least one test split channel and at least one control split channel from the first channel and binding of the analyte to the capture reagents provided in the reaction portion; washing the sample from the at least one test split channel and the at least one control split channel; introducing the reactant solution into the at least one test split channel and at least one control split channel; receiving by a test detection portion of the at least one test split channel and a control detection portion of the at least one control split channel residual reactant solution; determining a difference in volume of the test detection portion and the control detection portion; and correlating the difference in volume with the difference in initial number of reagent coated microparticles and unbound reagent coated microparticles. introducing the sample at an inlet port of a first channel; distributing the sample between at least one test split channel and at least one control split channel from the first channel and binding of the analyte to the capture reagents provided in the reaction portion; washing the sample from the at least one test split channel and the at least one control split channel; introducing the reactant solution into the at least one test split channel and at least one control split channel; receiving by a test detection portion of the at least one test split channel and a control detection portion of the at least one control split channel residual reactant solution; determining a difference in volume of the test detection portion and the control detection portion; and correlating the difference in volume with the difference in initial number of reagent coated microparticles and unbound reagent coated microparticles.