mhurhangee/ep-ind-claims huggingface dataset:
A method for generating a starter culture comprising at least two bacteriophage resistant variant strains, comprising the steps of: (a) exposing a parent bacterial strain comprising at least a portion of a CRISPR locus to a bacteriophage to produce a mixture of bacteria comprising a bacteriophage resistant variant strain comprising a modified CRISPR locus comprising at least one additional spacer in said modified CRISPR locus; (b) independently exposing the same parent bacterial strain as in step (a) comprising at least a portion of a CRISPR locus to the same bacteriophage as in step (a) to produce a mixture of bacteria comprising another bacteriophage resistant variant strain comprising a modified CRISPR locus comprising at least one additional spacer in said modified CRISPR locus; (c) selecting said bacteriophage resistant variant strains from said mixtures of bacteria; (d) selecting said bacteriophage resistant variant strains comprising an additional spacer in said modified CRISPR locus from said bacteriophage resistant strains selected in step (c); and (e) isolating said bacteriophage resistant variant strains, wherein said strains comprises an additional spacer in said modified CRISPR locus; wherein the sequence of the at least one additional spacer in the bacteriophage resistant variant strain is different to the sequence of the at least one additional spacer in the other bacteriophage resistant strain. (a) exposing a parent bacterial strain comprising at least a portion of a CRISPR locus to a bacteriophage to produce a mixture of bacteria comprising a bacteriophage resistant variant strain comprising a modified CRISPR locus comprising at least one additional spacer in said modified CRISPR locus; (b) independently exposing the same parent bacterial strain as in step (a) comprising at least a portion of a CRISPR locus to the same bacteriophage as in step (a) to produce a mixture of bacteria comprising another bacteriophage resistant variant strain comprising a modified CRISPR locus comprising at least one additional spacer in said modified CRISPR locus; (c) selecting said bacteriophage resistant variant strains from said mixtures of bacteria; (d) selecting said bacteriophage resistant variant strains comprising an additional spacer in said modified CRISPR locus from said bacteriophage resistant strains selected in step (c); and (e) isolating said bacteriophage resistant variant strains, wherein said strains comprises an additional spacer in said modified CRISPR locus; wherein the sequence of the at least one additional spacer in the bacteriophage resistant variant strain is different to the sequence of the at least one additional spacer in the other bacteriophage resistant strain.
A security container as safe, treasure-chest, security cabinet or strongbox for storing money, securities and/or valuables, which comprises - a housing with an opening at the front side, said housing further comprising a lower wall, two side walls (5, 6), a rear wall and an upper wall, and - a door pivoted to said housing and provided to close said opening, said door further comprising bolts arranged in pairs at opposite sides of the door and/or on the top and bottom of said door, said walls (4, 5, 6, 7, 8) being formed by an inner and an outer side, between said inner and said outer side of side walls (5, 6) at least one reinforcing element (12, 13) is arranged, said reinforcing element (12, 13) being a reinforcing profile arranged in vertical direction, or a reinforcing mash, characterized in that on said inner and/or said outer side of each side wall (5, 6) at least one weakened area of said sides (10, 11) is formed, in that at least one weakened area (16, 17) of the reinforcing element is formed on at least one reinforcing element (12, 13), in that each of the contact edges of said two inner sides of said side walls (5, 6) with said inner side of said bottom and said upper wall of said security container is externally reinforced with at least one reinforcing element, and in that a lower bolt at the hinge side of the door and the lower bolt at the opposite side of the door are located close to said lower wall of said security container, and that said upper bolt at the hinge side of the door and the upper bolt at the opposite side of the door are located close to said upper wall of said security container. - a housing with an opening at the front side, said housing further comprising a lower wall, two side walls (5, 6), a rear wall and an upper wall, and - a door pivoted to said housing and provided to close said opening, said door further comprising bolts arranged in pairs at opposite sides of the door and/or on the top and bottom of said door,
An internal combustion engine comprising a turbocharger (4, 16), an intercooler and a cooling circuit for cooling of the intercooler, the cooling circuit comprising adjusting means for adjusting a temperature of a cooling liquid of the cooling circuit flowing through the intercooler, the internal combustion engine comprising a controller for controlling the adjusting means of the cooling circuit, the controller comprising a function for determining a dew point temperature of the charge air, characterized in that the controller is configured to control the temperature of the cooling liquid and/or of the intercooler relative to the dew point temperature, wherein the internal combustion engine further comprises a temperature sensor for sensing the temperature of the coolant arranged in the cooling circuit or a temperature sensor for sensing the temperature of the intercooler arranged at the intercooler, the controller further comprising a feedback function for comparing the temperature sensed by the temperature sensor with a dew point temperature threshold, the controller being configured to control the adjusting means in dependence on the output of the feedback function. the cooling circuit comprising adjusting means for adjusting a temperature of a cooling liquid of the cooling circuit flowing through the intercooler, the internal combustion engine comprising a controller for controlling the adjusting means of the cooling circuit, the controller comprising a function for determining a dew point temperature of the charge air, characterized in that the controller is configured to control the temperature of the cooling liquid and/or of the intercooler relative to the dew point temperature, wherein the internal combustion engine further comprises a temperature sensor for sensing the temperature of the coolant arranged in the cooling circuit or a temperature sensor for sensing the temperature of the intercooler arranged at the intercooler, the controller further comprising a feedback function for comparing the temperature sensed by the temperature sensor with a dew point temperature threshold, the controller being configured to control the adjusting means in dependence on the output of the feedback function.
An apparatus for monitoring a vascular access, comprising: a fluid transport system for moving a fluid; at least a needle (3; 17, 18) connected to the fluid transport system and to a vascular access of an individual for supplying and/or removing the fluid to and/or from the vascular access via the at least a needle (3; 17, 18); a perturbation generator (5; 14) configured for generating variations of at least a parameter in the fluid transport system and/or for generating variations of at least a parameter in the needle (3; 17, 18), wherein the parameter comprises one or more chemical and/or physical characteristics of the fluid; a sensor (6; 19; 22; 26) configured for application to the individual's body, the sensor (6; 19; 22; 26) emitting a signal indicating the variations of the at least a parameter transmitted through the needle (3; 17, 18); an analysis device (7; 20) configured for recognising a detachment of the at least a needle (3; 17, 18), on a basis of said signal, characterized in that the at least a parameter is selected from a group comprising: pressure, flow, hematocrit, concentration of a substance in the fluid, optical properties, optical absorption, optical reflection, and velocity of sound propagation, and that the sensor (6; 19; 22; 26) comprises at least an emitter (23; 27) of light radiations and a receiver (24; 28) of radiations, the emitter (23; 27) of light radiations and the receiver (24; 28) of radiations being configured to be operatively associated to at least a tissue of the individual's body. a fluid transport system for moving a fluid; at least a needle (3; 17, 18) connected to the fluid transport system and to a vascular access of an individual for supplying and/or removing the fluid to and/or from the vascular access via the at least a needle (3; 17, 18); a perturbation generator (5; 14) configured for generating variations of at least a parameter in the fluid transport system and/or for generating variations of at least a parameter in the needle (3; 17, 18), wherein the parameter comprises one or more chemical and/or physical characteristics of the fluid; a sensor (6; 19; 22; 26) configured for application to the individual's body, the sensor (6; 19; 22; 26) emitting a signal indicating the variations of the at least a parameter transmitted through the needle (3; 17, 18); an analysis device (7; 20) configured for recognising a detachment of the at least a needle (3; 17, 18), on a basis of said signal, characterized in that the at least a parameter is selected from a group comprising: pressure, flow, hematocrit, concentration of a substance in the fluid, optical properties, optical absorption, optical reflection, and velocity of sound propagation, and that the sensor (6; 19; 22; 26) comprises at least an emitter (23; 27) of light radiations and a receiver (24; 28) of radiations, the emitter (23; 27) of light radiations and the receiver (24; 28) of radiations being configured to be operatively associated to at least a tissue of the individual's body.
An epigenetic modulator of DUX4 gene expression for use in a method of treating facioscapulohumeral muscular dystrophy (FSHD), the method comprising administering to a subject an effective amount of the epigenetic modulator, wherein the epigenetic modulator reduces DUX4 gene expression in the muscle cells of the subject, wherein the epigenetic modulator is an inhibitor of CARM1, and wherein the epigenetic modulator of DUX4 is an interfering nucleic acid targeting CARM1 and selected from the group consisting of double stranded RNA (dsRNA), siRNA, shRNA, and antisense oligonucleotide.
A method of conveying transport block size, TBS, information from a first node to a second node, comprising: determining one or more TBS parameters for a wireless transmission of data between a first node and a second node based on a first number of transmission layers associated with the wireless transmission, wherein the TBS parameters are to be used in decoding one or more transport blocks transmitted as part of the wireless transmission; generating a control message indicating the TBS parameters; transmitting the control message to the second node; transmitting at least one transport block to the second node in accordance with the TBS parameters indicated by the control message, wherein the transport block is transmitted over the first number of transmission layers; characterized by that the method further comprising retransmitting the at least one transport block to the second node in accordance with the TBS parameters indicated by the control message, wherein the transport block is retransmitted over a second number of transmission layers that differs from the first number of transmission layers. determining one or more TBS parameters for a wireless transmission of data between a first node and a second node based on a first number of transmission layers associated with the wireless transmission, wherein the TBS parameters are to be used in decoding one or more transport blocks transmitted as part of the wireless transmission; generating a control message indicating the TBS parameters; transmitting the control message to the second node; transmitting at least one transport block to the second node in accordance with the TBS parameters indicated by the control message, wherein the transport block is transmitted over the first number of transmission layers; characterized by that the method further comprising retransmitting the at least one transport block to the second node in accordance with the TBS parameters indicated by the control message, wherein the transport block is retransmitted over a second number of transmission layers that differs from the first number of transmission layers.
A process for preparing aminopolycarboxylates proceeding from an amino acid which, in a - first process step is reacted with ethylene oxide to give an intermediate mixture comprising the corresponding dialkanolamine, and then the intermediate mixture, in a - second process step is converted catalytically using a base to the corresponding aminopolycarboxylate, wherein - the amino acid, before the reaction with ethylene oxide in the first process step, is supplied to a partial neutralization with 0.70 to 0.99 equivalent of base per acid group, or, in the first process step, an amino acid which has already been partly neutralized with 0.70 to 0.99 equivalent of base per acid group is used. - first process step is reacted with ethylene oxide to give an intermediate mixture comprising the corresponding dialkanolamine, and then the intermediate mixture, in a - second process step is converted catalytically using a base to the corresponding aminopolycarboxylate, wherein - the amino acid, before the reaction with ethylene oxide in the first process step, is supplied to a partial neutralization with 0.70 to 0.99 equivalent of base per acid group, or, in the first process step, an amino acid which has already been partly neutralized with 0.70 to 0.99 equivalent of base per acid group is used.
Stamp for embossing or cutting out, comprising a plate on which at least one handle is arranged, wherein the plate has a top surface and a base surface opposite the top surface and an edge surface connecting the top surface and the base surface and on which at least one projection protrudes out of the base surface and/or at least one indentation protrudes into the latter, wherein the handle is connected to the plate in an articulated manner and can be transferred from a rest position into a usage position and vice versa, wherein the handle and the plate are formed in one piece from a plastic, wherein the handle is formed as a bow handle, which is secured to the plate via two joints spaced apart from each other and formed as film hinges, and wherein the film hinges are formed by a material weakening between plate and handle, and wherein the handle, in its rest position, encloses the plate at least partly at the edge.
A method for treating a fused-silica based optical component comprising: exposing the fused-silica based optical component to a first aqueous solution of mineral acids, the first aqueous solution including at least one of nitric acid (HNO 3), hydrochloric acid (HCl), perchloric acid (HClO 4), sulphuric acid (H 2 SO 4), or phosphoric acid (H 3 PO 4); heating the first aqueous solution including the fused-silica based optical component to a temperature in the range of 50° C. and 120° C.; placing the fused-silica based optical component in a second aqueous solution, the second aqueous solution including species of one of hydrofluoric acid, fluoride ions, or bifluoride ions, the aqueous solution having an etch rate; removing a surface layer from a region including optical damage precursors, using the second aqueous solution, to minimize etch-related optical damage precursor by-products; and agitating the second aqueous solution using an ultrasonic or megasonic method. exposing the fused-silica based optical component to a first aqueous solution of mineral acids, the first aqueous solution including at least one of nitric acid (HNO 3), hydrochloric acid (HCl), perchloric acid (HClO 4), sulphuric acid (H 2 SO 4), or phosphoric acid (H 3 PO 4); heating the first aqueous solution including the fused-silica based optical component to a temperature in the range of 50° C. and 120° C.; placing the fused-silica based optical component in a second aqueous solution, the second aqueous solution including species of one of hydrofluoric acid, fluoride ions, or bifluoride ions, the aqueous solution having an etch rate; removing a surface layer from a region including optical damage precursors, using the second aqueous solution, to minimize etch-related optical damage precursor by-products; and agitating the second aqueous solution using an ultrasonic or megasonic method.
A piezoelectric MEMS transducer (520; 820), formed in a body of semiconductor material having a central axis and a peripheral area (30A; 230A), the body comprising: a plurality of beams (539; 839) extending transversely to the central axis and having a first end (541; 841) and a second end (542; 842), the first end of the beams being coupled to the peripheral area of the body and the second end facing the central axis; a membrane (532; 832) extending transversely to the central axis and underneath the plurality of beams; and a pillar (534; 834) extending parallel to the central axis and connected to the second end of the beams and the membrane; the transducer further comprising a plurality of piezoelectric sensing elements (550; 550'; 850; 850') arranged on the plurality of beams, the plurality of piezoelectric sensing elements (550; 550'; 850; 850') comprising a first subset of piezoelectric sensing elements (550; 850), each arranged at the first end (541; 841) of a respective beam, characterized in that the plurality of piezoelectric sensing elements (550'; 850') further comprises a second subset of piezoelectric sensing elements (550'; 850'), each arranged at the second end (542; 842) of a respective beam. a plurality of beams (539; 839) extending transversely to the central axis and having a first end (541; 841) and a second end (542; 842), the first end of the beams being coupled to the peripheral area of the body and the second end facing the central axis; a membrane (532; 832) extending transversely to the central axis and underneath the plurality of beams; and a pillar (534; 834) extending parallel to the central axis and connected to the second end of the beams and the membrane; the transducer further comprising a plurality of piezoelectric sensing elements (550; 550'; 850; 850') arranged on the plurality of beams, the plurality of piezoelectric sensing elements (550; 550'; 850; 850') comprising a first subset of piezoelectric sensing elements (550; 850), each arranged at the first end (541; 841) of a respective beam, characterized in that the plurality of piezoelectric sensing elements (550'; 850') further comprises a second subset of piezoelectric sensing elements (550'; 850'), each arranged at the second end (542; 842) of a respective beam.
A collapsible metal box, in particular for transporting a material that is susceptible to creep, comprising a. a base, b. two first and two second opposing side panels (4a, 4b) that are articulated to the base, at least one of the first side panels comprising holding means for holding it in a position standing by itself on the base while the second side panels are folded out fully toward the outside characterized in that the holding means comprise a joining element of which - the lower part is temporarily inserted into the upper end of a corner upright of the base in order to hold the corresponding first side panel in the position standing by itself, and - the upper part is inserted into an opening at the lower end of a side upright of the first panel. a. a base, b. two first and two second opposing side panels (4a, 4b) that are articulated to the base, at least one of the first side panels comprising holding means for holding it in a position standing by itself on the base while the second side panels are folded out fully toward the outside characterized in that the holding means comprise a joining element of which - the lower part is temporarily inserted into the upper end of a corner upright of the base in order to hold the corresponding first side panel in the position standing by itself, and - the upper part is inserted into an opening at the lower end of a side upright of the first panel. - the lower part is temporarily inserted into the upper end of a corner upright of the base in order to hold the corresponding first side panel in the position standing by itself, and - the upper part is inserted into an opening at the lower end of a side upright of the first panel.
A system for delivering an agent or a combination of agents, comprising: a microdevice comprising an array of microstructures, wherein the microdevice has an aspect ratio of about 10:1 or higher, an application mechanism for applying the microdevice to an area of skin to generate a prepared area of skin comprising a plurality of nanopores or nanochannels in stratum corneum of the prepared area of skin, and a delivery mechanism for causing the agent or combination of agents to be delivered through the nanopores or nanochannels in the stratum corneum of the prepared area of skin, wherein the application mechanism and/or delivery mechanism comprises a mechanical applicator, an ultrasound applicator, a mechanical vibrator, an iontophoresis device, a sonophoresis device, a radiofrequency device, or a heat generating device. a microdevice comprising an array of microstructures, wherein the microdevice has an aspect ratio of about 10:1 or higher, an application mechanism for applying the microdevice to an area of skin to generate a prepared area of skin comprising a plurality of nanopores or nanochannels in stratum corneum of the prepared area of skin, and a delivery mechanism for causing the agent or combination of agents to be delivered through the nanopores or nanochannels in the stratum corneum of the prepared area of skin, wherein the application mechanism and/or delivery mechanism comprises a mechanical applicator, an ultrasound applicator, a mechanical vibrator, an iontophoresis device, a sonophoresis device, a radiofrequency device, or a heat generating device.