A method for determining a radius of gyration (Rg) of a particle in solution using a light scattering detector, the method comprising: passing the particle in solution through a flowpath in a sample cell, wherein the flowpath has a centerline aligned with a beam of light of the detector; determining an angular normalization factor (N θ1) for a first angle of the detector and an angular normalization factor (N θ2) of a second angle of the detector, wherein the first angle is 90° relative to the centerline, and wherein the second angle is 170° relative to the centerline; obtaining a first scattering intensity (I θ1) of the particle in solution at the first angle; obtaining a second scattering intensity (I θ2) of the particle in solution at the second angle; obtaining a 10° scattering intensity (I 10) of the particle in solution at an angle of 10°; determining a first particle scattering factor (P θ1) with the first scattering intensity (I θ1), the 10° scattering intensity (I 10), and the angular normalization factor (N θ1) for the first angle; determining a second particle scattering factor (P θ2) with the second scattering intensity (I θ2), the 10° scattering intensity (I 10), and the angular normalization factor (N θ2) for the second angle; plotting an angular dissymmetry plot, wherein the angular dissymmetry plot comprises the first particle scattering factor (P θ1) and the second particle scattering factor (P θ2); fitting a line to the angular dissymmetry plot; determining a slope of the line at a selected location on the line; determining the radius of gyration (Rg) of the particle in solution from the slope of the line; and outputting the radius of gyration (Rg). passing the particle in solution through a flowpath in a sample cell, wherein the flowpath has a centerline aligned with a beam of light of the detector; determining an angular normalization factor (N θ1) for a first angle of the detector and an angular normalization factor (N θ2) of a second angle of the detector, wherein the first angle is 90° relative to the centerline, and wherein the second angle is 170° relative to the centerline; obtaining a first scattering intensity (I θ1) of the particle in solution at the first angle; obtaining a second scattering intensity (I θ2) of the particle in solution at the second angle; obtaining a 10° scattering intensity (I 10) of the particle in solution at an angle of 10°; determining a first particle scattering factor (P θ1) with the first scattering intensity (I θ1), the 10° scattering intensity (I 10), and the angular normalization factor (N θ1) for the first angle; determining a second particle scattering factor (P θ2) with the second scattering intensity (I θ2), the 10° scattering intensity (I 10), and the angular normalization factor (N θ2) for the second angle; plotting an angular dissymmetry plot, wherein the angular dissymmetry plot comprises the first particle scattering factor (P θ1) and the second particle scattering factor (P θ2); fitting a line to the angular dissymmetry plot; determining a slope of the line at a selected location on the line; determining the radius of gyration (Rg) of the particle in solution from the slope of the line; and outputting the radius of gyration (Rg).