mhurhangee/ep-ind-claims huggingface dataset:
A method for amplifying DNA, comprising: (a) contacting one or more template strands of single-stranded DNA with one or more oligonucleotide primers complementary to a portion of the one or more template strands; (b) hybridizing at least one of the primers to a complementary portion of the one or more template strands; (c) contacting the one or more template strands with a DNA polymerase and at least four different nucleoside triphosphates, (d) extending the at least one hybridized primer by the DNA polymerase thereby forming one or more extension products bound to the one or more template strands; (e) separating the one or more extension product from the one or more template strands; and repeating steps (a), (b), (c), (d), and (e) one or more times, to amplify the DNA, wherein precision control is applied during at least one cycle of steps (b)-(d) by an application of stress to the one or more template strands, wherein stress is applied to the one or more template strands by directly applying mechanical tension to anchored DNA to stretch or elongate the DNA wherein the one or more primers contain activatable groups and the extension product comprises the activatable group of the primer and the method further comprises step (f) of activating the activatable groups to anchor the extension product and wherein step (f) is repeated until a desired degree of amplification is obtained. (a) contacting one or more template strands of single-stranded DNA with one or more oligonucleotide primers complementary to a portion of the one or more template strands; (b) hybridizing at least one of the primers to a complementary portion of the one or more template strands; (c) contacting the one or more template strands with a DNA polymerase and at least four different nucleoside triphosphates, (d) extending the at least one hybridized primer by the DNA polymerase thereby forming one or more extension products bound to the one or more template strands; (e) separating the one or more extension product from the one or more template strands;
A method of negotiating Quality of Service, QoS, within a network, wherein the method is performed by a network service provider and the method comprises: receiving a QoS reservation request from a client, the QoS reservation request comprising user information; sending a QoS control policy request to a content service provider, the QoS control policy request comprising the user information; receiving from the content service provider a QoS control policy response based on a QoS policy of the content service provider, in response to the QoS control policy request; determining a QoS reservation response based on both the QoS control policy response and a network QoS policy; and sending the QoS reservation response to the client; wherein the QoS reservation response comprises a guaranteed bit rate, GBR, and/or a maximum bit rate, MBP. receiving a QoS reservation request from a client, the QoS reservation request comprising user information; sending a QoS control policy request to a content service provider, the QoS control policy request comprising the user information; receiving from the content service provider a QoS control policy response based on a QoS policy of the content service provider, in response to the QoS control policy request; determining a QoS reservation response based on both the QoS control policy response and a network QoS policy; and sending the QoS reservation response to the client; wherein the QoS reservation response comprises a guaranteed bit rate, GBR, and/or a maximum bit rate, MBP.
Semi-conductor device of transistor with strained channel insulated from the substrate, comprising: - a substrate, - a transistor with a channel zone, a source zone, and a drain zone, said source and drain zones applying a mechanical strain over said channel zone, - a gate, separated from said channel by a gate insulator, - an insulating layer (14, 15, 121, 122, 123) made of an oxide of a given porous semi-conductor, the insulating layer extending under the transistor and comprising sidewalls (121, 122, 123) formed on either side of the source zone and the drain zone of the transistor, - the channel zone being arranged on said insulating layer, the drain and source zones being formed on a region of said insulating layer and arranged between the sidewalls made of the oxide of the given porous semi-conductor. - a substrate, - a transistor with a channel zone, a source zone, and a drain zone, said source and drain zones applying a mechanical strain over said channel zone, - a gate, separated from said channel by a gate insulator, - an insulating layer (14, 15, 121, 122, 123) made of an oxide of a given porous semi-conductor, the insulating layer extending under the transistor and comprising sidewalls (121, 122, 123) formed on either side of the source zone and the drain zone of the transistor, - the channel zone being arranged on said insulating layer, the drain and source zones being formed on a region of said insulating layer and arranged between the sidewalls made of the oxide of the given porous semi-conductor.
A mobile device comprising: a processor, a memory coupled to the processor, a display, an input mechanism (210, 216, 220), a first communication interface configured for communication with a cellular network or a wireless data network, a second communication interface configured to receive information related to an available parking space broadcast from a first local sensor node in communication range of the mobile device wherein the available parking space is detected by a second local sensor node different from the first local sensor node, said information including a location of the available parking; wherein the processor is configured to 1) determine a current location of the mobile device; 2) output a map to the display, said map including streets, the location of the mobile device and the location of the available parking space; 3) receive a request to initiate a transaction to purchase parking via the input mechanism (210, 216, 220), characterized in that the processor is further configured to 4) generate the transaction to purchase the parking via one or more communications with a remote device via the first communication interface; and 5) receive an electronic receipt from the remote device indicating the parking is purchased wherein the mobile device is configured to only receive communications from and not send communications to the first local sensor node.
Device for cooking foods with grill, comprising a grill, a removable structure supporting said grill, said structure comprises a bottom wall and at least one side wall defining an inner volume of said structure and wherein an opening is defined on said at least one side wall in order to allow the entrance of heat in said structure, said heat being emanated by an external heat source to the device and the device comprises a connector element between the external heat source and the opening wherein said connector element is internally hollow and comprises an inlet mouth directable towards the external source and an outlet mouth inserted in the opening.
A method of handling signaling messages at a transit network, the transit network connected to at least two LTE mobile networks, the method comprising: receiving, at the transit network, a session control message from a mobile network; determining, at the transit network, a signaling type associated with the session control message; and routing the session control message based on the signaling type determined; wherein routing the session control message includes routing the session control message based on domain routing if the determined signaling type is roaming signaling and routing the session control message includes routing the session control message based on called party routing if the determined signaling type is non-roaming signaling. receiving, at the transit network, a session control message from a mobile network; determining, at the transit network, a signaling type associated with the session control message; and routing the session control message based on the signaling type determined; wherein routing the session control message includes routing the session control message based on domain routing if the determined signaling type is roaming signaling and routing the session control message includes routing the session control message based on called party routing if the determined signaling type is non-roaming signaling.
A method for manufacturing a light emitting device, the method comprising: providing a wafer that includes, successively from an upper face side, an electrode structure that includes multilayer wiring, a semiconductor layer electrically connected to the electrode structure, and a growth substrate for the semiconductor layer; bonding the wafer to a support substrate; exposing the semiconductor layer by removing the growth substrate from the wafer that has been bonded to the support substrate; separating the semiconductor layer into a plurality of light emitting elements, which comprises forming grooves on a semiconductor layer side surface of the wafer; and forming a phosphor layer having protrusions and recesses at a surface thereof such that the phosphor layer covers the surfaces of the light emitting elements, which comprises: forming a coating film on the surfaces of the light emitting elements by applying a slurry comprising phosphor particles (a) contained in a solvent (c), and after forming the coating film, vaporizing the solvent (c) in the coating film to form the phosphor layer, wherein a thickness of the phosphor layer at a protrusion among the protrusions and the recesses is at least twice a thickness of the phosphor layer at a recess among the protrusions and the recesses in at least one given region of the light emitting element. providing a wafer that includes, successively from an upper face side, an electrode structure that includes multilayer wiring, a semiconductor layer electrically connected to the electrode structure, and a growth substrate for the semiconductor layer; bonding the wafer to a support substrate; exposing the semiconductor layer by removing the growth substrate from the wafer that has been bonded to the support substrate; separating the semiconductor layer into a plurality of light emitting elements, which comprises forming grooves on a semiconductor layer side surface of the wafer; and forming a phosphor layer having protrusions and recesses at a surface thereof such that the phosphor layer covers the surfaces of the light emitting elements, which comprises: forming a coating film on the surfaces of the light emitting elements by applying a slurry comprising phosphor particles (a) contained in a solvent (c), and after forming the coating film, vaporizing the solvent (c) in the coating film to form the phosphor layer, wherein a thickness of the phosphor layer at a protrusion among the protrusions and the recesses is at least twice a thickness of the phosphor layer at a recess among the protrusions and the recesses in at least one given region of the light emitting element. forming a coating film on the surfaces of the light emitting elements by applying a slurry comprising phosphor particles (a) contained in a solvent (c), and after forming the coating film, vaporizing the solvent (c) in the coating film to form the phosphor layer, wherein a thickness of the phosphor layer at a protrusion among the protrusions and the recesses is at least twice a thickness of the phosphor layer at a recess among the protrusions and the recesses in at least one given region of the light emitting element.
A device for extraction of plasma from a liquid sample, comprising: a first absorbent member that wicks the liquid sample; a second absorbent member that retains a fixed, predetermined quantity of plasma; a separator placed in physical contact between the first absorbent member and the second absorbent member for extracting plasma from the liquid sample; a first side of the separator is comprised of a first membrane and a second side of the separator is comprised of a second membrane; the first membrane is comprised of a lower porosity construction with enhanced hydrophilic properties relative to the first absorbent member, facilitating capillary action from the first absorbent member to the first membrane; the second membrane is comprised of a higher porosity construction and lesser hydrophilic properties relative to the second absorbent member, facilitating capillary action from the second membrane to the second absorbent member; wherein: the plasma loaded second absorbent member is dry-transferred for assay. a first absorbent member that wicks the liquid sample; a second absorbent member that retains a fixed, predetermined quantity of plasma; a separator placed in physical contact between the first absorbent member and the second absorbent member for extracting plasma from the liquid sample; a first side of the separator is comprised of a first membrane and a second side of the separator is comprised of a second membrane; the first membrane is comprised of a lower porosity construction with enhanced hydrophilic properties relative to the first absorbent member, facilitating capillary action from the first absorbent member to the first membrane; the second membrane is comprised of a higher porosity construction and lesser hydrophilic properties relative to the second absorbent member, facilitating capillary action from the second membrane to the second absorbent member; wherein: the plasma loaded second absorbent member is dry-transferred for assay.
A method for performing competitive binding analysis of two analytes comprising: preparing a control sample of a first analyte by pulling a first sample of said first analyte into a sample holding line, pulling a second sample of said first analyte into said sample holding line; injecting said control sample through a flow cell comprising an SPR sensor, said SPR sensor having a sensing region with an immobilized ligand; measuring the binding interaction of the first analyte in the control sample with said ligand using SPR analysis to generate a first binding interaction curve; preparing a binding competitive analysis dispersion gradient by pulling a sample comprising the first analyte and a second analyte into the sample holding line, followed by pulling a sample of the first analyte into the sample holding line thereby forming the binding competitive analysis dispersion gradient; pushing the resulting binding competitive analysis dispersion gradient through said flow cell; measuring the binding interaction of the binding competitive analysis dispersion gradient with said ligand using SPR analysis to generate a second binding interaction curve; subtracting the first binding interaction curve generated by the control sample from the second binding interaction curve generated by the competitive analysis dispersion gradient to produce an analyte binding response curve for the second analyte to the ligand in the presence of the first analyte. preparing a control sample of a first analyte by pulling a first sample of said first analyte into a sample holding line, pulling a second sample of said first analyte into said sample holding line; injecting said control sample through a flow cell comprising an SPR sensor, said SPR sensor having a sensing region with an immobilized ligand; measuring the binding interaction of the first analyte in the control sample with said ligand using SPR analysis to generate a first binding interaction curve; preparing a binding competitive analysis dispersion gradient by pulling a sample comprising the first analyte and a second analyte into the sample holding line, followed by pulling a sample of the first analyte into the sample holding line thereby forming the binding competitive analysis dispersion gradient; pushing the resulting binding competitive analysis dispersion gradient through said flow cell; measuring the binding interaction of the binding competitive analysis dispersion gradient with said ligand using SPR analysis to generate a second binding interaction curve; subtracting the first binding interaction curve generated by the control sample from the second binding interaction curve generated by the competitive analysis dispersion gradient to produce an analyte binding response curve for the second analyte to the ligand in the presence of the first analyte.
A method of monitoring an individual blade adjustment of a wind energy installation, which comprises a tower, a rotor arranged on the tower with at least two rotor blades, whose blade angle can be adjusted individually, and a blade adjustment system, wherein the blade adjustment system comprises a blade controller and sensors for sensing individual actual blade angles θ i act of the at least two rotor blades, wherein an individual blade controller is comprised, which receives a collective set point blade angle θ c set from the blade controller and which controls the at least two rotor blades respectively with individual set point blade angles θ i IPC, characterized in that at least one difference signal is formed by forming the difference between at least two collective blade angle signals ( θ act, θ IPC, θ c set) which are different from each other and which describe a state of the collective of the rotor blades, and is monitored as regards whether at least one threshold is passed in absolute terms, wherein the at least one difference signal to be monitored and/or the collective blade angle signals ( θ act, θ IPC, θ c set) which serve as the basis of the forming of the difference, and/or the individual blade angle signals θ i act, θ i IPC, θ i sim which serve as the basis of an or the collective blade angle signals ( θ act, θ IPC) is or are averaged over time.
A method of increasing the efficiency of a combustion process comprising the step of adding sodium bentonite to a flame, fireball or burner region combustion zone during combustion of a fuel, thereby increasing the radiant heat of combustion per unit of fuel, wherein the sodium bentonite comprises the following characteristics as determined by X-ray fluorescence: Consitituent Approx % by weight SiO 2 60-66 Al 2 O 3 19-22 Fe 2 O 3 3-4 TiO 2 0.10-0.2 P 2 O 5 0.03-0.08 CaO 0.4-1 MgO 1.7-2.5 SO 3 0.5-0.9 Na 2 O 1.3-2.5 K 2 O 0.3-0.6 BaO 0-0.05 SrO 0.01-0.04 V 2 O 5 <0.03 NiO 0-0.09 MnO 0.005-0.02 Cr 2 O 3 <0.01 Loss, fusion 0-15
Method for aligning a sensor device, which has a transmitter unit with at least one transmitter emitting light beams and a receiver unit with at least one receiver associated with the transmitter,which receives the light beams of the transmitter, in a working operation of the sensor device, characterised in that an alignment receiver is placed in front of the receiver to align the transmitter unit with the receiver unit, in that the light beams emitted by the transmitter are received by the alignment receiver and that displays signals, depending on the light quantity of the light beams received at the alignment receiver, using a display unit.
Apparatus for detecting different subjects (10, 10') on the basis of vital signs, comprising: - an image detection unit for detecting radiation from a field of view and for providing image data from the field of view, - a detection unit for defining image sections in the image data and for detecting movement patterns in each of the different image sections, - an identification unit for identifying vital signs in each of the different image sections on the basis of the movement patterns, wherein the identification unit is adapted to derive an alternating signal from the respective movement pattern for each of the image sections and wherein the identification unit comprises a frequency analysis device for analysing a frequency spectrum of each of the alternating signals, wherein the frequency analysis device is adapted to determine a spectral parameter on the basis of each of the alternating signals and to identify an image section as an image section including vital sign information if the spectral parameter exceeds a predefined threshold level, and - an analysis unit for analyzing the image data, wherein the analysis unit is adapted to combine connected image sections (48, 50), in which vital signs are identified, to a first group of image sections, wherein the analysis unit is adapted to combine connected image sections (54, 56), in which vital signs are identified and which are separated from the first group of image sections, to a second group of image sections, and wherein the analysis unit is adapted to detect the different subjects (10, 10') in the field of view on the basis of the groups of image sections (52, 58) in which the vital signs are identified, the first group of image sections corresponding to a first subject and the second group of image sections corresponding to a second subject. - an image detection unit for detecting radiation from a field of view and for providing image data from the field of view, - a detection unit for defining image sections in the image data and for detecting movement patterns in each of the different image sections, - an identification unit for identifying vital signs in each of the different image sections on the basis of the movement patterns, wherein the identification unit is adapted to derive an alternating signal from the respective movement pattern for each of the image sections and wherein the identification unit comprises a frequency analysis device for analysing a frequency spectrum of each of the alternating signals, wherein the frequency analysis device is adapted to determine a spectral parameter on the basis of each of the alternating signals and to identify an image section as an image section including vital sign information if the spectral parameter exceeds a predefined threshold level, and - an analysis unit for analyzing the image data,
A book comprising: front and back covers (2,3); a plurality of pages between the covers; an array of m × n, wherein m>1 and n>1, capacitive touch elements disposed on the inside (10,14) of a cover configured to provide a touchpad; at least one output device; and a controller operatively connected to the capacitive touch elements and configured to determine an x,y position of a user's digit on a page overlying the cover in dependence upon which capacitive touch elements are actuated and to cause the or at least one of the output devices to output a signal in dependence upon the x,y position. front and back covers (2,3); a plurality of pages between the covers; an array of m × n, wherein m>1 and n>1, capacitive touch elements disposed on the inside (10,14) of a cover configured to provide a touchpad; at least one output device; and a controller operatively connected to the capacitive touch elements and configured to determine an x,y position of a user's digit on a page overlying the cover in dependence upon which capacitive touch elements are actuated and to cause the or at least one of the output devices to output a signal in dependence upon the x,y position.