A method of making C4 acid derivative compound, comprising, obtaining a clarified fermentation broth from growth of a microorganism to produce at least one diammonium salt of a C4 diacid selected from the group consisting of succinic, malic, maleic and fumaric acid and wherein the clarified fermentation broth containing the ammonium salt of a C4 diacid Is obtained by: - growing a microorganism In a fermentation media to produce a fermentation broth containing the C4 diacid; - contacting the fermentation broth with the C4 diacid with a calcium compound to form a calcium salt of the diacid; - contacting the fermentation broth with a source of ammonium and with a source of carbonate under a first condition including a temperature of at least 100°C and a pressure of at least 1480.28 kPa [200 psig] for a time sufficient to form a carbonate salt of at least 90% of the calcium and an ammonium salt of at least 90% of the C4 diacid in the fermentation broth; - reducing the temperature and pressure of the contacted fermentation broth to a second condition effective to form a precipitate of the carbonate salt of calcium while maintaining the ammonium salt of the diacid in solution; and - separating the precipitated carbonate salt of the calcium and cell mass in the fermentation broth from the solution of the ammonium salt of the diacid to form the clarified fermentation broth containing the ammonium salt of a C4 diacid; and contacting the clarified fermentation broth containing the diammonium salt of the C4 diacid with a hydrogenation catalyst and H 2 in a reaction mixture under conditions of temperature, pressure and time sufficient to hydrogenate the diammonium salt of the C4 diacid to form a derivative compound selected from the group consisting of gamma-butyrolactone and 1,4-butane-diol. obtaining a clarified fermentation broth from growth of a microorganism to produce at least one diammonium salt of a C4 diacid selected from the group consisting of succinic, malic, maleic and fumaric acid and wherein the clarified fermentation broth containing the ammonium salt of a C4 diacid Is obtained by: - growing a microorganism In a fermentation media to produce a fermentation broth containing the C4 diacid; - contacting the fermentation broth with the C4 diacid with a calcium compound to form a calcium salt of the diacid; - contacting the fermentation broth with a source of ammonium and with a source of carbonate under a first condition including a temperature of at least 100°C and a pressure of at least 1480.28 kPa [200 psig] for a time sufficient to form a carbonate salt of at least 90% of the calcium and an ammonium salt of at least 90% of the C4 diacid in the fermentation broth; - reducing the temperature and pressure of the contacted fermentation broth to a second condition effective to form a precipitate of the carbonate salt of calcium while maintaining the ammonium salt of the diacid in solution; and - separating the precipitated carbonate salt of the calcium and cell mass in the fermentation broth from the solution of the ammonium salt of the diacid to form the clarified fermentation broth containing the ammonium salt of a C4 diacid; and - growing a microorganism In a fermentation media to produce a fermentation broth containing the C4 diacid; - contacting the fermentation broth with the C4 diacid with a calcium compound to form a calcium salt of the diacid; - contacting the fermentation broth with a source of ammonium and with a source of carbonate under a first condition including a temperature of at least 100°C and a pressure of at least 1480.28 kPa [200 psig] for a time sufficient to form a carbonate salt of at least 90% of the calcium and an ammonium salt of at least 90% of the C4 diacid in the fermentation broth; - reducing the temperature and pressure of the contacted fermentation broth to a second condition effective to form a precipitate of the carbonate salt of calcium while maintaining the ammonium salt of the diacid in solution; and - separating the precipitated carbonate salt of the calcium and cell mass in the fermentation broth from the solution of the ammonium salt of the diacid to form the clarified fermentation broth containing the ammonium salt of a C4 diacid; and contacting the clarified fermentation broth containing the diammonium salt of the C4 diacid with a hydrogenation catalyst and H 2 in a reaction mixture under conditions of temperature, pressure and time sufficient to hydrogenate the diammonium salt of the C4 diacid to form a derivative compound selected from the group consisting of gamma-butyrolactone and 1,4-butane-diol.