An analysis cartridge (100, 200) comprising: a sealing film, a cartridge having a first surface and a second surface, and a first cover covering the first surface, said cover having a through hole, said cartridge having: - an opening, - a liquid storage box with an unvented liquid storage chamber disposed therein for storing a diluent, said liquid storage box having a liquid through-hole, - a liquid channel, and - at least one reaction chamber for storing a reactive substance, wherein the liquid channel interconnects the at least one reaction chamber with the unvented liquid storage chamber via said liquid through hole; wherein the sealing film is configured to pass through the through hole of the first cover and through the opening of the cartridge; wherein a first terminal of the sealing film is configured to seal the unvented liquid storage chamber by sealing the through-hole of the liquid storage box to avoid diluent flowing out when analysis is not yet performed, and a second terminal of the sealing film is configured to be pulled by an operator to expose the liquid through hole and the unvented storage chamber when analysis is performed; wherein the sealing film penetrates the opening and the through hole by way of reversely bending the sealing film when analysis is not yet performed; and wherein, when analysis is not yet performed, the first terminal of the sealing film has a folded portion which overlaps the through hole, and the second terminal is configured to pass through the through hole of the first cover and through the opening, such to be upward extended out of the cartridge. a sealing film, a cartridge having a first surface and a second surface, and a first cover covering the first surface, said cover having a through hole, said cartridge having: - an opening, - a liquid storage box with an unvented liquid storage chamber disposed therein for storing a diluent, said liquid storage box having a liquid through-hole, - a liquid channel, and - at least one reaction chamber for storing a reactive substance, - an opening, - a liquid storage box with an unvented liquid storage chamber disposed therein for storing a diluent, said liquid storage box having a liquid through-hole, - a liquid channel, and - at least one reaction chamber for storing a reactive substance, wherein the liquid channel interconnects the at least one reaction chamber with the unvented liquid storage chamber via said liquid through hole; wherein the sealing film is configured to pass through the through hole of the first cover and through the opening of the cartridge; wherein a first terminal of the sealing film is configured to seal the unvented liquid storage chamber by sealing the through-hole of the liquid storage box to avoid diluent flowing out when analysis is not yet performed, and a second terminal of the sealing film is configured to be pulled by an operator to expose the liquid through hole and the unvented storage chamber when analysis is performed; wherein the sealing film penetrates the opening and the through hole by way of reversely bending the sealing film when analysis is not yet performed; and wherein, when analysis is not yet performed, the first terminal of the sealing film has a folded portion which overlaps the through hole, and the second terminal is configured to pass through the through hole of the first cover and through the opening, such to be upward extended out of the cartridge.