A method for processing an image comprising spots generated during gene sequencing comprising: an image pre-processing step, comprising: analyzing a to-be processed image so as to obtain a first image, wherein the to-be-processed image comprises at least one spot having at least one pixel point; and a spot detecting step, comprising: analyzing the first image so as to compute a spot determining threshold; analyzing the first image so as to acquire a candidate spot; and characterized in that the method further comprises: judging whether the candidate spot meets the spot determining threshold, comprising: searching in the first image for a pixel point having a maximum intensity I max within a p ∗ p window as a center of the candidate spot, wherein the center of the candidate spot has a connectivity with (p ∗ p-1) surrounding pixels in the p ∗ p window; and judging whether the center of the candidate spot satisfies a condition of I max ∗ A BI ∗ ceof guass > T, where A BI is a proportion of pixels in the first image being a set value in the p ∗ p window, ceof guass is a correlation coefficient between pixels in the p ∗ p window and a two-dimensional Gaussian distribution, and T is the spot determining threshold, where p is an odd number greater than 1; determining a spot corresponding to the center of the candidate spot as the spot when the condition is satisfied; and discarding the spot corresponding to the center of the candidate spot when the condition fails to be satisfied. an image pre-processing step, comprising: analyzing a to-be processed image so as to obtain a first image, wherein the to-be-processed image comprises at least one spot having at least one pixel point; and a spot detecting step, comprising: analyzing the first image so as to compute a spot determining threshold; analyzing the first image so as to acquire a candidate spot; and characterized in that the method further comprises: judging whether the candidate spot meets the spot determining threshold, comprising: searching in the first image for a pixel point having a maximum intensity I max within a p ∗ p window as a center of the candidate spot, wherein the center of the candidate spot has a connectivity with (p ∗ p-1) surrounding pixels in the p ∗ p window; and judging whether the center of the candidate spot satisfies a condition of I max ∗ A BI ∗ ceof guass > T, where A BI is a proportion of pixels in the first image being a set value in the p ∗ p window, ceof guass is a correlation coefficient between pixels in the p ∗ p window and a two-dimensional Gaussian distribution, and T is the spot determining threshold, where p is an odd number greater than 1; determining a spot corresponding to the center of the candidate spot as the spot when the condition is satisfied; and discarding the spot corresponding to the center of the candidate spot when the condition fails to be satisfied. analyzing the first image so as to compute a spot determining threshold; analyzing the first image so as to acquire a candidate spot; and characterized in that the method further comprises: judging whether the candidate spot meets the spot determining threshold, comprising: searching in the first image for a pixel point having a maximum intensity I max within a p ∗ p window as a center of the candidate spot, wherein the center of the candidate spot has a connectivity with (p ∗ p-1) surrounding pixels in the p ∗ p window; and judging whether the center of the candidate spot satisfies a condition of I max ∗ A BI ∗ ceof guass > T, where A BI is a proportion of pixels in the first image being a set value in the p ∗ p window, ceof guass is a correlation coefficient between pixels in the p ∗ p window and a two-dimensional Gaussian distribution, and T is the spot determining threshold, where p is an odd number greater than 1; determining a spot corresponding to the center of the candidate spot as the spot when the condition is satisfied; and discarding the spot corresponding to the center of the candidate spot when the condition fails to be satisfied. searching in the first image for a pixel point having a maximum intensity I max within a p ∗ p window as a center of the candidate spot, wherein the center of the candidate spot has a connectivity with (p ∗ p-1) surrounding pixels in the p ∗ p window; and judging whether the center of the candidate spot satisfies a condition of I max ∗ A BI ∗ ceof guass > T, where A BI is a proportion of pixels in the first image being a set value in the p ∗ p window, ceof guass is a correlation coefficient between pixels in the p ∗ p window and a two-dimensional Gaussian distribution, and T is the spot determining threshold, where p is an odd number greater than 1; determining a spot corresponding to the center of the candidate spot as the spot when the condition is satisfied; and discarding the spot corresponding to the center of the candidate spot when the condition fails to be satisfied.