A method of manufacturing a lithographic printing plate, which comprises the steps of: imagewise exposing a lithographic printing plate precursor comprising (a) a support, (b) provided thereon an image-recording layer having a negative photosensitive layer which is a radically polymerizable photosensitive layer containing a polymerizable compound, a polymerization initiator and a hydrophobic binder polymer, and which is hardened in the exposed area, and (c) a protective layer cutting off diffusing invasion of oxygen, provided on the photosensitive layer; simultaneously and by only one bath performing a desensitizing treatment; and removing a non-image area of the image-recording layer in a development processing tank that has a reservoir of a first aqueous solution having pH of 2-10 by rubbing the non-image area with at least one rubbing member in a state of being immersed in the first aqueous solution, which step is carried out (i) after an elapse of ≥ 2 seconds after immersion, and (ii) at least at intermediate or a latter half of the time when the lithographic printing plate is immersed in the first aqueous solution; and then drying the lithographic printing plate precursor. imagewise exposing a lithographic printing plate precursor comprising (a) a support, (b) provided thereon an image-recording layer having a negative photosensitive layer which is a radically polymerizable photosensitive layer containing a polymerizable compound, a polymerization initiator and a hydrophobic binder polymer, and which is hardened in the exposed area, and (c) a protective layer cutting off diffusing invasion of oxygen, provided on the photosensitive layer; (a) a support, (b) provided thereon an image-recording layer having a negative photosensitive layer which is a radically polymerizable photosensitive layer containing a polymerizable compound, a polymerization initiator and a hydrophobic binder polymer, and which is hardened in the exposed area, and (c) a protective layer cutting off diffusing invasion of oxygen, provided on the photosensitive layer; simultaneously and by only one bath performing a desensitizing treatment; and removing a non-image area of the image-recording layer in a development processing tank that has a reservoir of a first aqueous solution having pH of 2-10 by rubbing the non-image area with at least one rubbing member in a state of being immersed in the first aqueous solution, which step is carried out (i) after an elapse of ≥ 2 seconds after immersion, and (ii) at least at intermediate or a latter half of the time when the lithographic printing plate is immersed in the first aqueous solution; and then performing a desensitizing treatment; and removing a non-image area of the image-recording layer in a development processing tank that has a reservoir of a first aqueous solution having pH of 2-10 by rubbing the non-image area with at least one rubbing member in a state of being immersed in the first aqueous solution, which step is carried out (i) after an elapse of ≥ 2 seconds after immersion, and (ii) at least at intermediate or a latter half of the time when the lithographic printing plate is immersed in the first aqueous solution; and then drying the lithographic printing plate precursor.