An apparatus comprising: an enclosure configured to hold a first liquid medium disposed on a first surface in a first section of said enclosure and a second liquid medium disposed on an electrowetting surface in a second section of said enclosure, wherein said enclosure comprises: a first microfluidic channel; a second microfluidic channel; microfluidic holding pens, each holding pen connected to one or both of the first and second channels; and a boundary between said first section and said second section of said enclosure, said boundary comprising a physical barrier located in said enclosure between said first section of said enclosure and said second section of said enclosure and a passage from said first section of said enclosure through said barrier to said second section of said enclosure; wherein: said first section of said enclosure comprises a DEP configuration configured to induce selectively net dielectrophoresis forces in said first liquid medium sufficiently to capture and move, relative to said first surface, micro-objects in said first liquid medium in said first section of said enclosure while connected to a biasing device, and said second section of said enclosure comprises an electrowetting configuration configured to change selectively an effective wetting characteristic of regions of said electrowetting surface sufficiently to move a liquid droplet within said second medium in said second section of said enclosure while connected to a biasing device. a first microfluidic channel; a second microfluidic channel; microfluidic holding pens, each holding pen connected to one or both of the first and second channels; and a boundary between said first section and said second section of said enclosure, said boundary comprising a physical barrier located in said enclosure between said first section of said enclosure and said second section of said enclosure and a passage from said first section of said enclosure through said barrier to said second section of said enclosure; wherein: said first section of said enclosure comprises a DEP configuration configured to induce selectively net dielectrophoresis forces in said first liquid medium sufficiently to capture and move, relative to said first surface, micro-objects in said first liquid medium in said first section of said enclosure while connected to a biasing device, and said second section of said enclosure comprises an electrowetting configuration configured to change selectively an effective wetting characteristic of regions of said electrowetting surface sufficiently to move a liquid droplet within said second medium in said second section of said enclosure while connected to a biasing device. said first section of said enclosure comprises a DEP configuration configured to induce selectively net dielectrophoresis forces in said first liquid medium sufficiently to capture and move, relative to said first surface, micro-objects in said first liquid medium in said first section of said enclosure while connected to a biasing device, and said second section of said enclosure comprises an electrowetting configuration configured to change selectively an effective wetting characteristic of regions of said electrowetting surface sufficiently to move a liquid droplet within said second medium in said second section of said enclosure while connected to a biasing device.