A system for evaluating a fresh tissue sample, prepared as to fluoresce under illumination and removed or exposed during a medical procedure, the system comprising: a stage for holding the fresh tissue sample; a structured light source comprising a light source and a spatial light modulator, wherein: the light source is configured to generate a light beam; and the spatial light modulator is configured to modulate the light beam according to a predefined set of multiple illumination patterns, wherein the structured light source is configured to project the multiple illumination patterns of the light beam onto the fresh tissue sample to illuminate the fresh tissue sample; an imaging system configured to image fluorescence emitted from the fresh tissue sample when the stage and at least a portion of the imaging system are arranged at a first relative position and illuminated with the predefined set of multiple illumination patterns to provide a first optically sectioned composite image and to image fluorescence emitted from the fresh tissue sample when the stage and at least a portion of the imaging system are arranged at a second relative position and illuminated with the predefined set of multiple illumination patterns to provide a second optically sectioned composite image; and a system control configured to provide a human-comprehensible clinical image having sufficient resolution to allow for meaningful clinical decision making associated with the medical procedure based on the clinical image by synthesizing at least the first optically sectioned composite image and the second optically sectioned composite image. a stage for holding the fresh tissue sample; a structured light source comprising a light source and a spatial light modulator, wherein: the light source is configured to generate a light beam; and the spatial light modulator is configured to modulate the light beam according to a predefined set of multiple illumination patterns, wherein the structured light source is configured to project the multiple illumination patterns of the light beam onto the fresh tissue sample to illuminate the fresh tissue sample; an imaging system configured to image fluorescence emitted from the fresh tissue sample when the stage and at least a portion of the imaging system are arranged at a first relative position and illuminated with the predefined set of multiple illumination patterns to provide a first optically sectioned composite image and to image fluorescence emitted from the fresh tissue sample when the stage and at least a portion of the imaging system are arranged at a second relative position and illuminated with the predefined set of multiple illumination patterns to provide a second optically sectioned composite image; and a system control configured to provide a human-comprehensible clinical image having sufficient resolution to allow for meaningful clinical decision making associated with the medical procedure based on the clinical image by synthesizing at least the first optically sectioned composite image and the second optically sectioned composite image. the light source is configured to generate a light beam; and the spatial light modulator is configured to modulate the light beam according to a predefined set of multiple illumination patterns, wherein the structured light source is configured to project the multiple illumination patterns of the light beam onto the fresh tissue sample to illuminate the fresh tissue sample; an imaging system configured to image fluorescence emitted from the fresh tissue sample when the stage and at least a portion of the imaging system are arranged at a first relative position and illuminated with the predefined set of multiple illumination patterns to provide a first optically sectioned composite image and to image fluorescence emitted from the fresh tissue sample when the stage and at least a portion of the imaging system are arranged at a second relative position and illuminated with the predefined set of multiple illumination patterns to provide a second optically sectioned composite image; and a system control configured to provide a human-comprehensible clinical image having sufficient resolution to allow for meaningful clinical decision making associated with the medical procedure based on the clinical image by synthesizing at least the first optically sectioned composite image and the second optically sectioned composite image.