A method for detecting bed bugs, comprising: (a) contacting a sample comprising a bed bug antigen with: (i) an anti-bed bug monoclonal antibody produced by a hybridoma deposited at the American Type Culture Collection (ATCC) under Accession Number PTA-122644, or an antigen-binding fragment thereof, or an anti-bed bug monoclonal antibody produced by a hybridoma deposited at the ATCC under Accession Number PTA-122645, or an antigen-binding fragment thereof, (ii) a conjugated anti-bed bug monoclonal antibody or conjugated antigen-binding fragment comprising: an anti-bed bug monoclonal antibody produced by a hybridoma deposited at the American Type Culture Collection (ATCC) under Accession Number PTA-122644, or an antigen-binding fragment thereof, and a detection agent, or an anti-bed bug monoclonal antibody produced by a hybridoma deposited at the ATCC under Accession Number PTA-122645, or an antigen-binding fragment thereof, and a detection agent, or (iii) a combination thereof, and (b) detecting binding of the bed bug antigen to the anti-bed bug monoclonal antibody or antigen-binding fragment, conjugated anti-bed bug monoclonal antibody or conjugated antigen-binding fragment, or combination thereof, wherein the detecting comprises visual analysis of a colorimetric, fluorescent, or luminescent reaction or use of a device that measures such reactions. (a) contacting a sample comprising a bed bug antigen with: (i) an anti-bed bug monoclonal antibody produced by a hybridoma deposited at the American Type Culture Collection (ATCC) under Accession Number PTA-122644, or an antigen-binding fragment thereof, or an anti-bed bug monoclonal antibody produced by a hybridoma deposited at the ATCC under Accession Number PTA-122645, or an antigen-binding fragment thereof, (ii) a conjugated anti-bed bug monoclonal antibody or conjugated antigen-binding fragment comprising: an anti-bed bug monoclonal antibody produced by a hybridoma deposited at the American Type Culture Collection (ATCC) under Accession Number PTA-122644, or an antigen-binding fragment thereof, and a detection agent, or an anti-bed bug monoclonal antibody produced by a hybridoma deposited at the ATCC under Accession Number PTA-122645, or an antigen-binding fragment thereof, and a detection agent, or (iii) a combination thereof, and (i) an anti-bed bug monoclonal antibody produced by a hybridoma deposited at the American Type Culture Collection (ATCC) under Accession Number PTA-122644, or an antigen-binding fragment thereof, or an anti-bed bug monoclonal antibody produced by a hybridoma deposited at the ATCC under Accession Number PTA-122645, or an antigen-binding fragment thereof, (ii) a conjugated anti-bed bug monoclonal antibody or conjugated antigen-binding fragment comprising: an anti-bed bug monoclonal antibody produced by a hybridoma deposited at the American Type Culture Collection (ATCC) under Accession Number PTA-122644, or an antigen-binding fragment thereof, and a detection agent, or an anti-bed bug monoclonal antibody produced by a hybridoma deposited at the ATCC under Accession Number PTA-122645, or an antigen-binding fragment thereof, and a detection agent, or (iii) a combination thereof, and (b) detecting binding of the bed bug antigen to the anti-bed bug monoclonal antibody or antigen-binding fragment, conjugated anti-bed bug monoclonal antibody or conjugated antigen-binding fragment, or combination thereof, wherein the detecting comprises visual analysis of a colorimetric, fluorescent, or luminescent reaction or use of a device that measures such reactions.