A method for generating a microscopy panoramic representation (10, 10') of a three-dimensional sample having a lateral extension in the x and y directions, comprising the steps of: a) arranging the three-dimensional sample on a positioner; b) recording the sample by means of an imaging device in order to obtain a first microscopy picture (12a, 12a', 12a") of the sample having a first section; c) altering the perspective onto the two-dimensional sample in the z direction, wherein an alteration in the z direction comprises an alteration of the magnification factor; d) recording the sample by means of the imaging device in order to obtain a second microscopy picture (12b, 12b', 12b") of the sample having a second section; e) determining change information which allows drawing conclusions as to the change in perspective in the z direction, using a difference between the first and second microscopy pictures (12a, 12a', 12a", 12b, 12b', 12b"); and f) merging the first and second microscopy pictures (12a, 12a', 12a", 12b, 12b', 12b") while considering the change information in order to obtain the microscopy panoramic representation (10, 10'); wherein the first and second microscopy pictures (12a, 12a', 12a", 12b, 12b', 12b") or a region of the first microscopy picture and a region of the second microscopy picture (12a, 12a', 12a", 12b, 12b', 12b") differ as regards a magnification factor, and wherein the method comprises the step of inserting the first or second microscopy picture (12a, 12a', 12a", 12b, 12b', 12b") into the microscopy panoramic representation (10, 10') or of inserting the region of the first microscopy picture (12a, 12a', 12a") or of inserting the region of the second microscopy picture (12b, 12b', 12b") into the microscopy panoramic representation (10, 10'), and wherein that microscopy picture (12a, 12a', 12a", 12b, 12b', 12b") or that region of the microscopy picture (12a, 12a', 12a", 12b, 12b', 12b") comprising the greater magnification factor will be introduced; wherein the method comprises the step of detecting a change in objective by means of a threshold value method and blob finding in order to determine the magnification factor; or wherein the method comprises the step of determining the magnification factor using registerable regions in an overlapping section of the first and second microscopy pictures (12b, 12b', 12b"). a) arranging the three-dimensional sample on a positioner; b) recording the sample by means of an imaging device in order to obtain a first microscopy picture (12a, 12a', 12a") of the sample having a first section; c) altering the perspective onto the two-dimensional sample in the z direction, wherein an alteration in the z direction comprises an alteration of the magnification factor; d) recording the sample by means of the imaging device in order to obtain a second microscopy picture (12b, 12b', 12b") of the sample having a second section; e) determining change information which allows drawing conclusions as to the change in perspective in the z direction, using a difference between the first and second microscopy pictures (12a, 12a', 12a", 12b, 12b', 12b"); and f) merging the first and second microscopy pictures (12a, 12a', 12a", 12b, 12b', 12b") while considering the change information in order to obtain the microscopy panoramic representation (10, 10');