A preparation method of a ligand of adsorbing materials for adsorbing multiple pathogenic factors of sepsis in fluids, comprising the following steps: 1) In dichloromethane, di(2-cyanoethylamine) (compound 1 with n 2 = 2 and n 3 = 2) reacts with di-tert-butyl dicarbonate to generate compound 2, reaction temperature is 20∼30°C, the equivalence ratio of compound 1 and di-tert-butyl dicarbonate is 1:0.5∼2, reaction equation is 2) In a saturated solution of ammonia in methanol, compound 3 is generated from compound 2 through hydrogenation under the existence of Raney nickel and hydrogen, reaction temperature is 20∼50°C, pressure is 1∼10 Mpa, the mass of Raney nickel is 10%∼ 50% of the mass of compound 2, reaction equation is: 3) In ethanol or methanol, compound 3 reacts with acrylonitrile to generate compound 4 with n 1 = 2 and n 4 = 2, reaction temperature is 20∼50°C, the equivalence ratio of compound 3 and acrylonitrile is 1:2∼3, reaction equation is: 4) In dichloromethane, 3,4-dimethoxyhydrocinnamic acid (compound 5 with R 1 = H, R 2 = H, R 3 = OMe, R 4 = OMe, R 5 = H and n 5 = 2) reacts with N-Hydroxysuccinimide to generate compound 6 under the existence of N,N'-Dicyclohexylcarbodiimide and 4-dimethylaminopyridine, reaction temperature is 20∼30°C, the equivalence ratio of compound 5 and N-Hydroxysuccinimide is 1:1∼2, reaction equation is: 5) In dioxane, compound 4 reacts with compound 6 to generate compound 7, reaction temperature is 30∼50°C, the equivalence ratio of compound 4 and compound 6 is 1:1∼2, reaction equation is: 6) In dioxane, compound 7 reacts with N-Carbobenzoxyoxysuccinimide to generate compound 8, reaction temperature is 30∼50°C, the equivalence ratio of compound 7 and N-Carbobenzoxyoxysuccinimide is 1:1∼2, reaction equation is: 7) In ethanol or methanol, compound 8 reacts with di-tert-butyl dicarbonate to generate compound 9 under the existence of raney nickel and hydrogen, reaction temperature is 30∼50°C, the equivalence ratio of compound 8 and di-tert-butyl dicarbonate is 1:0.5∼3, pressure is 1∼10Mpa, the mass of the Raney nickel is 10%∼50% of the mass of compound 8, reaction equation is: 8) In methanol, compound 10 is generated from compound 9 under the existence of palladium on carbon and hydrogen, reaction temperature is 20∼ 50°C, pressure ranges from atmospheric pressure to 10 MPa, the mass of the Palladium on carbon is 10%∼30% of the mass of compound 9, reaction equation is: 9) In dichloromethane, compound 10 reacts with succinic anhydride to generate compound 11 under the existence of 4-dimethylaminopyridine, reaction temperature is 20∼30°C, the equivalence ratio of compound 10 and succinic anhydride is 1:1∼2, reaction equation is: 10) In ethyl acetate, compound 11 reacts with N-Hydroxysuccinimide to generate compound 12, reaction temperature is 20∼30°C, the equivalence ratio of compound 11 and N-Hydroxysuccinimide is 1:1∼2, reaction equation is: 1) In dichloromethane, di(2-cyanoethylamine) (compound 1 with n 2 = 2 and n 3 = 2) reacts with di-tert-butyl dicarbonate to generate compound 2, reaction temperature is 20∼30°C, the equivalence ratio of compound 1 and di-tert-butyl dicarbonate is 1:0.5∼2, reaction equation is 2) In a saturated solution of ammonia in methanol, compound 3 is generated from compound 2 through hydrogenation under the existence of Raney nickel and hydrogen, reaction temperature is 20∼50°C, pressure is 1∼10 Mpa, the mass of Raney nickel is 10%∼ 50% of the mass of compound 2, reaction equation is: 3) In ethanol or methanol, compound 3 reacts with acrylonitrile to generate compound 4 with n 1 = 2 and n 4 = 2, reaction temperature is 20∼50°C, the equivalence ratio of compound 3 and acrylonitrile is 1:2∼3, reaction equation is: 4) In dichloromethane, 3,4-dimethoxyhydrocinnamic acid (compound 5 with R 1 = H, R 2 = H, R 3 = OMe, R 4 = OMe, R 5 = H and n 5 = 2) reacts with N-Hydroxysuccinimide to generate compound 6 under the existence of N,N'-Dicyclohexylcarbodiimide and 4-dimethylaminopyridine, reaction temperature is 20∼30°C, the equivalence ratio of compound 5 and N-Hydroxysuccinimide is 1:1∼2, reaction equation is: 5) In dioxane, compound 4 reacts with compound 6 to generate compound 7, reaction temperature is 30∼50°C, the equivalence ratio of compound 4 and compound 6 is 1:1∼2, reaction equation is: 6) In dioxane, compound 7 reacts with N-Carbobenzoxyoxysuccinimide to generate compound 8, reaction temperature is 30∼50°C, the equivalence ratio of compound 7 and N-Carbobenzoxyoxysuccinimide is 1:1∼2, reaction equation is: 7) In ethanol or methanol, compound 8 reacts with di-tert-butyl dicarbonate to generate compound 9 under the existence of raney nickel and hydrogen, reaction temperature is 30∼50°C, the equivalence ratio of compound 8 and di-tert-butyl dicarbonate is 1:0.5∼3, pressure is 1∼10Mpa, the mass of the Raney nickel is 10%∼50% of the mass of compound 8, reaction equation is: 8) In methanol, compound 10 is generated from compound 9 under the existence of palladium on carbon and hydrogen, reaction temperature is 20∼ 50°C, pressure ranges from atmospheric pressure to 10 MPa, the mass of the Palladium on carbon is 10%∼30% of the mass of compound 9, reaction equation is: 9) In dichloromethane, compound 10 reacts with succinic anhydride to generate compound 11 under the existence of 4-dimethylaminopyridine, reaction temperature is 20∼30°C, the equivalence ratio of compound 10 and succinic anhydride is 1:1∼2, reaction equation is: 10) In ethyl acetate, compound 11 reacts with N-Hydroxysuccinimide to generate compound 12, reaction temperature is 20∼30°C, the equivalence ratio of compound 11 and N-Hydroxysuccinimide is 1:1∼2, reaction equation is: