A one step all-in-one apparatus for body fluid sampling and sensing, comprising: a housing, a plurality of integrated sampling and sensing assemblies, a cartridge, sealing films (101,102), an upper cover, a sampling port, a linear actuating mechanism, a control circuit and withdrawing springs; wherein, one integrated sampling and sensing assembly comprises a micro sampling needle, a needle hub and a test strip; the micro sampling needle is fixed on the needle hub, and the test strip is mounted on the lower surface of the needle hub, and a hole is set on the needle hub to enable the communication between the micro sampling needle and the test strip, and the plurality of integrated sampling and sensing assemblies are arranged in a radial configuration in the cartridge; the micro sampling needles are hollow microneedles, configured to penetrate the skin to sample the body fluid and deliver the body fluid to the test strips directly via the holes on the needle hubs, and a sensor is configured to read the changes occurred on the test strips due to the reaction of the body fluid with the reagents on the test strips, wherein sampling and sensing are completed in one step; the sealing films (101,102) are located on the upper and lower surfaces of the cartridge respectively, and a plurality of chambers are arranged in a radial configuration in the cartridge, wherein the upper and lower sealing films (101,102) and the plurality of chambers compose a plurality of sealed reaction chambers; the upper cover is located on the upper portion of the housing, and the sampling port is located on the upper cover, configured to allow the micro sampling needle to protrude from and withdraw into the housing; the linear actuating mechanism which is connected with the control circuit electrically is located below the cartridge, configured to actuate the integrated sampling and sensing assemblies allowing the micro sampling needles to penetrate the skin; the control circuit is configured to control the movements of the linear actuating mechanism and the integrated sampling and sensing assemblies so as to control the micro sampling needles to reach the designated depths under the skin; the withdrawing springs are set in the plurality of chambers, configured to withdraw the micro sampling needles after sampling; characterized in that, the linear actuating mechanism comprises a rod and a rod spring; the rod is a hollow rod, and a side hole is set on one end of the rod near the cartridge, configured to connect the lumen of the rod and the sealed reaction chamber after the rod penetrates the sealing film on the lower surface of the cartridge; a vacuum device is set on the other end of the rod away from the cartridge; the rod spring is configured to withdraw the rod after sampling of the body fluid of the integrated sampling and sensing assembly. a housing, a plurality of integrated sampling and sensing assemblies, a cartridge, sealing films (101,102), an upper cover, a sampling port, a linear actuating mechanism, a control circuit and withdrawing springs; wherein, one integrated sampling and sensing assembly comprises a micro sampling needle, a needle hub and a test strip; the micro sampling needle is fixed on the needle hub, and the test strip is mounted on the lower surface of the needle hub, and a hole is set on the needle hub to enable the communication between the micro sampling needle and the test strip, and the plurality of integrated sampling and sensing assemblies are arranged in a radial configuration in the cartridge; the micro sampling needles are hollow microneedles, configured to penetrate the skin to sample the body fluid and deliver the body fluid to the test strips directly via the holes on the needle hubs, and a sensor is configured to read the changes occurred on the test strips due to the reaction of the body fluid with the reagents on the test strips, wherein sampling and sensing are completed in one step; the sealing films (101,102) are located on the upper and lower surfaces of the cartridge respectively, and a plurality of chambers are arranged in a radial configuration in the cartridge, wherein the upper and lower sealing films (101,102) and the plurality of chambers compose a plurality of sealed reaction chambers; the upper cover is located on the upper portion of the housing, and the sampling port is located on the upper cover, configured to allow the micro sampling needle to protrude from and withdraw into the housing; the linear actuating mechanism which is connected with the control circuit electrically is located below the cartridge, configured to actuate the integrated sampling and sensing assemblies allowing the micro sampling needles to penetrate the skin; the control circuit is configured to control the movements of the linear actuating mechanism and the integrated sampling and sensing assemblies so as to control the micro sampling needles to reach the designated depths under the skin; the withdrawing springs are set in the plurality of chambers, configured to withdraw the micro sampling needles after sampling; characterized in that, the linear actuating mechanism comprises a rod and a rod spring; the rod is a hollow rod, and a side hole is set on one end of the rod near the cartridge, configured to connect the lumen of the rod and the sealed reaction chamber after the rod penetrates the sealing film on the lower surface of the cartridge; a vacuum device is set on the other end of the rod away from the cartridge; the rod spring is configured to withdraw the rod after sampling of the body fluid of the integrated sampling and sensing assembly.