An analysis chip (1, 1a to 1p, 2, 3, 4, 5, 100, 100a to 100r, 110, 200, 200a, 300, 400) comprising: a substrate made of a substrate main body (10b, 10d, 10e, 10f) having a plurality of reaction portions (11, 21, 41, 51) in which a selective binding substance selectively binding to a substance to be examined is immobilized, wherein the substrate has hydrophilicity at least at the main surface on which the reaction portions are formed a corner portion (C 1 -C 12) which is the outer edge of the main surface of the substrate main body; a partition portion (12, 12a-12i, 22, 42, 52, 701) formed on the surface of the substrate main body (10b, 10d, 10e, 10f) on which the reaction portions (11, 21, 41, 51) are provided, and a connection portion (13, 13a to 13d, 15, 15a, 15b, 16, 16a, 16b, 17, 17a, 23, 43, 61, 61a, 61b, 62, 62a, 62b, 63, 63a, 64, 64a, 65, 91, 93, 94) having water repellency, the connection portion being configured to connect between a part of the partition portion and the corner portion characterized in that said partition portion (12, 12a-12i, 22, 42, 52, 701) is provided on the main surface of the substrate partitioning the reaction portions (11, 21, 41, 51) by surrounding each of the reaction portions with a water repellent material inside the outer edge formed by the main surface, a strip-like shape water repellent surface formed of the partition portion (12, 12a-12i, 22, 42, 52, 701) and the connection portion (13, 13a to 13d, 15, 15a, 15b, 16, 16a, 16b, 17, 17a, 23, 43, 61, 61a, 61b, 62, 62a, 62b, 63, 63a, 64, 64a, 65, 91, 93, 94) is only formed on a part of the substrate, and the water repellent surface formed of the partition portion (12, 12a-12i, 22, 42, 52, 701) and the connection portion (13, 13a to 13d, 15, 15a, 15b, 16, 16a, 16b, 17, 17a, 23, 43, 61, 61a, 61b, 62, 62a, 62b, 63, 63a, 64, 64a, 65, 91, 93, 94) has a smaller occupied area than the main surface of the substrate. a substrate made of a substrate main body (10b, 10d, 10e, 10f) having a plurality of reaction portions (11, 21, 41, 51) in which a selective binding substance selectively binding to a substance to be examined is immobilized, wherein the substrate has hydrophilicity at least at the main surface on which the reaction portions are formed a corner portion (C 1 -C 12) which is the outer edge of the main surface of the substrate main body; a partition portion (12, 12a-12i, 22, 42, 52, 701) formed on the surface of the substrate main body (10b, 10d, 10e, 10f) on which the reaction portions (11, 21, 41, 51) are provided, and a connection portion (13, 13a to 13d, 15, 15a, 15b, 16, 16a, 16b, 17, 17a, 23, 43, 61, 61a, 61b, 62, 62a, 62b, 63, 63a, 64, 64a, 65, 91, 93, 94) having water repellency, the connection portion being configured to connect between a part of the partition portion and the corner portion characterized in that said partition portion (12, 12a-12i, 22, 42, 52, 701) is provided on the main surface of the substrate partitioning the reaction portions (11, 21, 41, 51) by surrounding each of the reaction portions with a water repellent material inside the outer edge formed by the main surface, a strip-like shape water repellent surface formed of the partition portion (12, 12a-12i, 22, 42, 52, 701) and the connection portion (13, 13a to 13d, 15, 15a, 15b, 16, 16a, 16b, 17, 17a, 23, 43, 61, 61a, 61b, 62, 62a, 62b, 63, 63a, 64, 64a, 65, 91, 93, 94) is only formed on a part of the substrate, and the water repellent surface formed of the partition portion (12, 12a-12i, 22, 42, 52, 701) and the connection portion (13, 13a to 13d, 15, 15a, 15b, 16, 16a, 16b, 17, 17a, 23, 43, 61, 61a, 61b, 62, 62a, 62b, 63, 63a, 64, 64a, 65, 91, 93, 94) has a smaller occupied area than the main surface of the substrate.