A method of producing a rotavirus like particle in a plant, portion of a plant or plant cell, comprising: a) introducing a first nucleic acid comprising a first regulatory region active in the plant operatively linked to a first nucleotide sequence encoding a first rotavirus structural protein selected from one of VP2, VP6 and VP7, a second nucleic acid comprising a second regulatory region active in the plant operatively linked to a second nucleotide sequence encoding a second rotavirus structural protein selected from one of VP2, VP6 and VP7 and a third nucleic acid comprising a third regulatory region active in the plant operatively linked to a third nucleotide sequence encoding a third rotavirus structural protein selected from one of VP2, VP6 and VP7 into the plant, portion of a plant or plant cell, wherein the first, second or third nucleotide sequence encoding the VP7 comprises a truncated native signal peptide or a non-native signal peptide from a plant polypeptide, and wherein the RLP comprises VP2, VP6 and VP7; or a) providing a plant, portion of a plant or plant cell comprising a first nucleic acid comprising a first regulatory region active in the plant operatively linked to a first nucleotide sequence encoding a first rotavirus structural protein, a second nucleic acid comprising a second regulatory region active in the plant operatively linked to a second nucleotide sequence encoding a second rotavirus structural protein and a third nucleic acid comprising a third regulatory region active in the plant operatively linked to a third nucleotide sequence encoding a third rotavirus structural protein into the plant, portion of a plant or plant cell; wherein the first rotavirus structural protein is VP2, the second rotavirus structural protein is VP6 and the third rotavirus structural protein is VP7, wherein VP7 comprises a truncated native signal peptide or a non-native signal peptide from a plant polypeptide; b) incubating the plant, portion of a plant or plant cell under conditions that permit the transient expression of the first, second and third nucleic acid, thereby producing the RLP, c) harvesting the plant, portion of the plant or plant cell, and d) extracting and purifying the RLPs from the plant, portion of the plant or plant cell, in the presence of calcium, wherein the concentration of calcium is between 1 mM and 1000 mM. a) introducing a first nucleic acid comprising a first regulatory region active in the plant operatively linked to a first nucleotide sequence encoding a first rotavirus structural protein selected from one of VP2, VP6 and VP7, a second nucleic acid comprising a second regulatory region active in the plant operatively linked to a second nucleotide sequence encoding a second rotavirus structural protein selected from one of VP2, VP6 and VP7 and a third nucleic acid comprising a third regulatory region active in the plant operatively linked to a third nucleotide sequence encoding a third rotavirus structural protein selected from one of VP2, VP6 and VP7 into the plant, portion of a plant or plant cell, wherein the first, second or third nucleotide sequence encoding the VP7 comprises a truncated native signal peptide or a non-native signal peptide from a plant polypeptide, and wherein the RLP comprises VP2, VP6 and VP7; or a) providing a plant, portion of a plant or plant cell comprising a first nucleic acid comprising a first regulatory region active in the plant operatively linked to a first nucleotide sequence encoding a first rotavirus structural protein, a second nucleic acid comprising a second regulatory region active in the plant operatively linked to a second nucleotide sequence encoding a second rotavirus structural protein and a third nucleic acid comprising a third regulatory region active in the plant operatively linked to a third nucleotide sequence encoding a third rotavirus structural protein into the plant, portion of a plant or plant cell; wherein the first rotavirus structural protein is VP2, the second rotavirus structural protein is VP6 and the third rotavirus structural protein is VP7, wherein VP7 comprises a truncated native signal peptide or a non-native signal peptide from a plant polypeptide; b) incubating the plant, portion of a plant or plant cell under conditions that permit the transient expression of the first, second and third nucleic acid, thereby producing the RLP, c) harvesting the plant, portion of the plant or plant cell, and d) extracting and purifying the RLPs from the plant, portion of the plant or plant cell, in the presence of calcium, wherein the concentration of calcium is between 1 mM and 1000 mM.