A dual construct system to express the coding sequence of a gene of interest in an host cell, said coding sequence consisting of a 5'end portion and of a 3'end portion, said dual construct system comprising: a) a first plasmid comprising in a 5'-3' direction: - a AAV 5'-inverted terminal repeat (5'-ITR) sequence; - a promoter sequence; - the 5' end portion of said coding sequence, said 5'end portion being operably linked to and under control of said promoter; - a nucleic acid sequence of a splicing donor signal; and - a AAV 3'-inverted terminal repeat (3'-ITR) sequence; and b) a second plasmid comprising in a 5'-3' direction: - a AAV 5'-inverted terminal repeat (5'-ITR) sequence; - a nucleic acid sequence of a splicing acceptor signal; - the 3'end of said coding sequence; - a poly-adenylation signal nucleic acid sequence; and - a AAV 3'-inverted terminal repeat (3'-ITR) sequence, wherein said first plasmid further comprises a nucleic acid sequence of a recombinogenic region in 5' position of the AAV 3'ITR of said first plasmid and in 3' position of the nucleic acid sequence of the splicing donor signal and wherein said second plasmid further comprises the nucleic acid sequence of the recombinogenic region in 3' position of the AAV 5'-ITR of said second plasmid and in 5' position of the nucleic acid sequence of the splicing acceptor signal, wherein the recombinogenic region is a F1 phage recombinogenic region that consists of the sequence: GGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGC GAATTTTAACAAAAT (SEQ ID NO. 3) or a fragment thereof that maintains the recombinogenic property of SEQ ID No. 3, wherein upon introduction of said first plasmid and said second plasmid into the host cell, said coding sequence reconstitutes by means of the splicing donor and the splicing acceptor signals. a) a first plasmid comprising in a 5'-3' direction: - a AAV 5'-inverted terminal repeat (5'-ITR) sequence; - a promoter sequence; - the 5' end portion of said coding sequence, said 5'end portion being operably linked to and under control of said promoter; - a nucleic acid sequence of a splicing donor signal; and - a AAV 3'-inverted terminal repeat (3'-ITR) sequence; and - a AAV 5'-inverted terminal repeat (5'-ITR) sequence; - a promoter sequence; - the 5' end portion of said coding sequence, said 5'end portion being operably linked to and under control of said promoter; - a nucleic acid sequence of a splicing donor signal; and - a AAV 3'-inverted terminal repeat (3'-ITR) sequence; and b) a second plasmid comprising in a 5'-3' direction: - a AAV 5'-inverted terminal repeat (5'-ITR) sequence; - a nucleic acid sequence of a splicing acceptor signal; - the 3'end of said coding sequence; - a poly-adenylation signal nucleic acid sequence; and - a AAV 3'-inverted terminal repeat (3'-ITR) sequence, - a AAV 5'-inverted terminal repeat (5'-ITR) sequence; - a nucleic acid sequence of a splicing acceptor signal; - the 3'end of said coding sequence; - a poly-adenylation signal nucleic acid sequence; and - a AAV 3'-inverted terminal repeat (3'-ITR) sequence, GGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGC GAATTTTAACAAAAT (SEQ ID NO. 3) or a fragment thereof that maintains the recombinogenic property of SEQ ID No. 3, wherein upon introduction of said first plasmid and said second plasmid into the host cell, said coding sequence reconstitutes by means of the splicing donor and the splicing acceptor signals.