A method for assembling a catheter assembly having a catheter adapter operably coupled to a catheter, wherein the catheter adapter and catheter each comprise various internal surfaces and geometries, comprising: providing a catheter assembly having an internal lumen; providing a septum having an outer surface, wherein the outer diameter of the septum is greater than an opening of the catheter adapter; coating the outer surface of the septum with an excess of an antimicrobial composition comprising: a non-alcohol, biocidal agent; a lubricant; and a carrier solvent comprising a non-alcohol based solvent in which the biocidal agent and lubricant are dissolved, and which readily undergoes evaporation at ambient conditions; inserting the coated septum into the internal lumen of the catheter adapter, wherein the septum compresses inwardly during insertion of the septum through the opening of the catheter adapter; distributing the excess antimicrobial composition to the internal lumen by advancing and seating the coated septum within the internal lumen, such that it is seated within a channel or groove formed on the inner surface of the catheter adapter; further distributing the excess antimicrobial composition within the internal lumen and forcing the excess antimicrobial composition between opposing surfaces of components within the catheter assembly by blowing air through the internal lumen and the seated septum; and blowing additional air through the internal lumen and the seated septum to evaporate the carrier solvent of the antimicrobial composition thereby immobilizing the lubricant and the non-alcohol, biocidal agent within the internal lumen. providing a catheter assembly having an internal lumen; providing a septum having an outer surface, wherein the outer diameter of the septum is greater than an opening of the catheter adapter; coating the outer surface of the septum with an excess of an antimicrobial composition comprising: a non-alcohol, biocidal agent; a lubricant; and a carrier solvent comprising a non-alcohol based solvent in which the biocidal agent and lubricant are dissolved, and which readily undergoes evaporation at ambient conditions; a non-alcohol, biocidal agent; a lubricant; and a carrier solvent comprising a non-alcohol based solvent in which the biocidal agent and lubricant are dissolved, and which readily undergoes evaporation at ambient conditions; inserting the coated septum into the internal lumen of the catheter adapter, wherein the septum compresses inwardly during insertion of the septum through the opening of the catheter adapter; distributing the excess antimicrobial composition to the internal lumen by advancing and seating the coated septum within the internal lumen, such that it is seated within a channel or groove formed on the inner surface of the catheter adapter; further distributing the excess antimicrobial composition within the internal lumen and forcing the excess antimicrobial composition between opposing surfaces of components within the catheter assembly by blowing air through the internal lumen and the seated septum; and blowing additional air through the internal lumen and the seated septum to evaporate the carrier solvent of the antimicrobial composition thereby immobilizing the lubricant and the non-alcohol, biocidal agent within the internal lumen.