A recombinant human α-galactosidase-A (rhAGA) protein having: a percentage of total N-linked oligosaccharides in peak 11 that are bis-mannose-6-phosphorylated oligosaccharides that is greater than 9%; a percentage of total N-linked oligosaccharides in peak 3 that are monosialylated, minus fucose oligosaccharides that is between 0.1% and 1.6%; a percentage of total N-linked oligosaccharides in peak 4 that are bisialylated fucose-containing oligosaccharides that is greater than 13.5 %; a percentage of total N-linked oligosaccharides in peak 6' that are triantennary, trisialylated oligosaccharides of form 2 that is greater than 2.0%; a percentage of total N-linked oligosaccharides in peak 1 that are neutrally-charged oligosaccharides that is between 0.1 % and 3.9%; a percentage of total N-linked oligosaccharides in peak 2 that are monosialylated fucosecontaining oligosaccharides that is between 0.1% and 3.0%; a percentage of total N-linked oligosaccharides in peak 5 that are bisialylated oligosaccharides that is between 0.1 % and 5.3%; a percentage of total N-linked oligosaccharides in peak 6 that are triantennary, trisialylated oligosaccharides of form 1 that is between 0.1% and 9.0%; a percentage of total N-linked oligosaccharides in peak 7 that are mannose-6-phosphorylated oligosaccharides that is between 1% and 7.0%; a percentage of total N-linked oligosaccharides in peak 8 that are monophosphorylated oligosaccharides that is greater than 14.8%; a percentage of total N-linked oligosaccharides in peak 9 that are tetrasialylated oligosaccharides that is greater than 4.9%; and a percentage of total N-linked oligosaccharides in peak 10 that are monosialylated and monophosphorylated oligosaccharides that is greater than 8.2%; wherein the peaks are determined by anthranilic acid labeled N-linked oligosaccharide profiling. a percentage of total N-linked oligosaccharides in peak 11 that are bis-mannose-6-phosphorylated oligosaccharides that is greater than 9%; a percentage of total N-linked oligosaccharides in peak 3 that are monosialylated, minus fucose oligosaccharides that is between 0.1% and 1.6%; a percentage of total N-linked oligosaccharides in peak 4 that are bisialylated fucose-containing oligosaccharides that is greater than 13.5 %; a percentage of total N-linked oligosaccharides in peak 6' that are triantennary, trisialylated oligosaccharides of form 2 that is greater than 2.0%; a percentage of total N-linked oligosaccharides in peak 1 that are neutrally-charged oligosaccharides that is between 0.1 % and 3.9%; a percentage of total N-linked oligosaccharides in peak 2 that are monosialylated fucosecontaining oligosaccharides that is between 0.1% and 3.0%; a percentage of total N-linked oligosaccharides in peak 5 that are bisialylated oligosaccharides that is between 0.1 % and 5.3%; a percentage of total N-linked oligosaccharides in peak 6 that are triantennary, trisialylated oligosaccharides of form 1 that is between 0.1% and 9.0%; a percentage of total N-linked oligosaccharides in peak 7 that are mannose-6-phosphorylated oligosaccharides that is between 1% and 7.0%; a percentage of total N-linked oligosaccharides in peak 8 that are monophosphorylated oligosaccharides that is greater than 14.8%; a percentage of total N-linked oligosaccharides in peak 9 that are tetrasialylated oligosaccharides that is greater than 4.9%; and a percentage of total N-linked oligosaccharides in peak 10 that are monosialylated and monophosphorylated oligosaccharides that is greater than 8.2%; wherein the peaks are determined by anthranilic acid labeled N-linked oligosaccharide profiling.