A method for culturing pluripotent stem cells comprising: a culturing process, which is performed in a liquid medium which does not substantially contain a basic fibroblast growth factor: the process of bringing pluripotent stem cells into contact with a polypeptide which has cell adhesion activity and includes a first domain for binding the basic fibroblast growth factor and a second domain for adhering to a culture vessel and culturing the pluripotent stem cells, wherein the basic fibroblast growth factor is bound to the first domain, and the second domain is adhered to the culture vessel, wherein the number of amino acid residues of the polypeptide is 80 to 250, wherein the first domain includes any one of the following sequence A-1, sequence A-2, and sequence A-3, and the second domain includes any one of the following sequence B-1, sequence B-2, and sequence B-3. Sequence A-1: an amino acid sequence represented by SEQ ID No: 2 Sequence A-2: an amino acid sequence which shares identity of equal to or higher than 80% with the amino acid sequence represented by SEQ ID No: 2 and has the ability to bind the basic fibroblast growth factor Sequence A-3: an amino acid sequence which is obtained by addition, substitution, or deletion of one or several amino acid residues in the amino acid sequence represented by SEQ ID No: 2 and has the ability to bind the basic fibroblast growth factor Sequence B-1: an amino acid sequence represented by SEQ ID No: 3 Sequence B-2: an amino acid sequence which shares identity of equal to or higher than 80% with the amino acid sequence represented by SEQ ID No: 3 and has the ability to adhere to the culture vessel Sequence B-3: an amino acid sequence which is obtained by addition, substitution, or deletion of one or several amino acid residues in the amino acid sequence represented by SEQ ID No: 3 and has the ability to adhere to the culture vessel. a culturing process, which is performed in a liquid medium which does not substantially contain a basic fibroblast growth factor: the process of bringing pluripotent stem cells into contact with a polypeptide which has cell adhesion activity and includes a first domain for binding the basic fibroblast growth factor and a second domain for adhering to a culture vessel and culturing the pluripotent stem cells, wherein the basic fibroblast growth factor is bound to the first domain, and the second domain is adhered to the culture vessel, wherein the number of amino acid residues of the polypeptide is 80 to 250, wherein the first domain includes any one of the following sequence A-1, sequence A-2, and sequence A-3, and the second domain includes any one of the following sequence B-1, sequence B-2, and sequence B-3. Sequence A-1: an amino acid sequence represented by SEQ ID No: 2 Sequence A-2: an amino acid sequence which shares identity of equal to or higher than 80% with the amino acid sequence represented by SEQ ID No: 2 and has the ability to bind the basic fibroblast growth factor Sequence A-3: an amino acid sequence which is obtained by addition, substitution, or deletion of one or several amino acid residues in the amino acid sequence represented by SEQ ID No: 2 and has the ability to bind the basic fibroblast growth factor Sequence B-1: an amino acid sequence represented by SEQ ID No: 3 Sequence B-2: an amino acid sequence which shares identity of equal to or higher than 80% with the amino acid sequence represented by SEQ ID No: 3 and has the ability to adhere to the culture vessel Sequence B-3: an amino acid sequence which is obtained by addition, substitution, or deletion of one or several amino acid residues in the amino acid sequence represented by SEQ ID No: 3 and has the ability to adhere to the culture vessel. Sequence A-1: an amino acid sequence represented by SEQ ID No: 2 Sequence A-2: an amino acid sequence which shares identity of equal to or higher than 80% with the amino acid sequence represented by SEQ ID No: 2 and has the ability to bind the basic fibroblast growth factor Sequence A-3: an amino acid sequence which is obtained by addition, substitution, or deletion of one or several amino acid residues in the amino acid sequence represented by SEQ ID No: 2 and has the ability to bind the basic fibroblast growth factor Sequence B-1: an amino acid sequence represented by SEQ ID No: 3 Sequence B-2: an amino acid sequence which shares identity of equal to or higher than 80% with the amino acid sequence represented by SEQ ID No: 3 and has the ability to adhere to the culture vessel Sequence B-3: an amino acid sequence which is obtained by addition, substitution, or deletion of one or several amino acid residues in the amino acid sequence represented by SEQ ID No: 3 and has the ability to adhere to the culture vessel.