A method for amplifying a target nucleic acid sequence t C -t V -t C ' comprised within a sequence tract t n -t C -t V -t C '-t n ', said method comprising conducting a plurality of amplification reactions, each reaction comprising - a first amplification step, whereby said target nucleic acid sequence is amplified using i. a left (forward) initial PCR primer having a sequence m a -K-p C and ii. a right (reverse) initial primer having a sequence and m a -K'-p C ', yielding a first amplificate, - a second amplification step, whereby said first amplificate is amplified using iii. a left (forward) adaptor PCR primer consisting of a sequence a L -a P -a K and iv. a right (reverse) adaptor PCR primer consisting of a sequence a L '-a P '-a K ', yielding a second amplificate, wherein - t V is a variable region within said target nucleic acid sequence, - p C is the same sequence as sequence element t C and p C ' is the reverse complimentary sequence to t C ', - K comprises a sequence element k 1 and a 3'-terminal sequence element S, and K' comprises a sequence element k 1 ' and a 3'-terminal sequence element S', wherein - k 1 and k 1 ' each independently from one another are a sequence 2 to 9 nucleotides in length, - S and S' are mismatch sequences selected not to form a continuous hybrid sequence with sequence element t n and t n ', and S and S' are each independently 1, 2, 3, 4 or 5 nucleotides in length, - a k is the same sequence as sequence element k 1 and a K ' is the same sequence as sequence element k 1 ', - a P -a K hybridizes to a contiguous sequence on m a -K and a P '-a K ' hybridizes to a contiguous sequence on m a -K', - p C, p C ', m a -K and m a -K' each independently from one another are a sequence 10 to 40 nucleotides in length, and a L and a L ' independently from one another can be any sequence, and wherein a particular set of primers for each one of said plurality of amplification reactions is provided, wherein in each of these sets of primers - k 1 of said particular set is different from any other k 1 in any of the other sets and/or - k 1 ' of said particular set is different from any other k 1 ' in any of the other sets. - a first amplification step, whereby said target nucleic acid sequence is amplified using i. a left (forward) initial PCR primer having a sequence m a -K-p C and ii. a right (reverse) initial primer having a sequence and m a -K'-p C ', yielding a first amplificate, i. a left (forward) initial PCR primer having a sequence m a -K-p C and ii. a right (reverse) initial primer having a sequence and m a -K'-p C ', yielding a first amplificate, - a second amplification step, whereby said first amplificate is amplified using iii. a left (forward) adaptor PCR primer consisting of a sequence a L -a P -a K and iv. a right (reverse) adaptor PCR primer consisting of a sequence a L '-a P '-a K ', yielding a second amplificate, iii. a left (forward) adaptor PCR primer consisting of a sequence a L -a P -a K and iv. a right (reverse) adaptor PCR primer consisting of a sequence a L '-a P '-a K ', - t V is a variable region within said target nucleic acid sequence, - p C is the same sequence as sequence element t C and p C ' is the reverse complimentary sequence to t C ', - K comprises a sequence element k 1 and a 3'-terminal sequence element S, and K' comprises a sequence element k 1 ' and a 3'-terminal sequence element S', wherein - k 1 and k 1 ' each independently from one another are a sequence 2 to 9 nucleotides in length, - S and S' are mismatch sequences selected not to form a continuous hybrid sequence with sequence element t n and t n ', and S and S' are each independently 1, 2, 3, 4 or 5 nucleotides in length, - k 1 and k 1 ' each independently from one another are a sequence 2 to 9 nucleotides in length, - S and S' are mismatch sequences selected not to form a continuous hybrid sequence with sequence element t n and t n ', and S and S' are each independently 1, 2, 3, 4 or 5 nucleotides in length, - a k is the same sequence as sequence element k 1 and a K ' is the same sequence as sequence element k 1 ', - a P -a K hybridizes to a contiguous sequence on m a -K and a P '-a K ' hybridizes to a contiguous sequence on m a -K', - p C, p C ', m a -K and m a -K' each independently from one another are a sequence 10 to 40 nucleotides in length, and a L and a L ' independently from one another can be any sequence, and wherein - k 1 of said particular set is different from any other k 1 in any of the other sets and/or - k 1 ' of said particular set is different from any other k 1 ' in any of the other sets.