An analyzing and reading device, for reading and analyzing a test strip for assay detection, the test strip having a detection zone and a blank zone, comprising: a processor; a photoelectric detection circuit, configured to detect signals of the strength of light reflection in the detection zone and the blank zone and feedback detection information to the processor; wherein the photoelectric detection circuit comprises: at least two light sources (61, 62), corresponding to positions of the detection zone and the blank zone of the test strip, and able to emit lights corresponding to the detection zone and blank zone of the test strip; and at least one optical detector, configured to receive reflected lights from the detection zone and the blank zone; wherein the lights emitted by the at least two light sources (61, 62) irradiate the detection zone and blank zone of the strip and are reflected therefrom, and then received by the optical detector, which in turn feedback the detection information to the processor; the device further comprising a diaphragm separating the at least two light sources (61, 62) and the at least one optical detector from one another, characterized in that the optical detector is a photoelectric sensor, the at least two light sources are comprised of a first light source and a second light source, wherein the lights emitted by the two light sources (61, 62) irradiate the detection zone and blank zone of the test strip, and then are received by the photoelectric sensor after reflection, and the analyzing and reading device further comprises a T-shaped diaphragm separating the first, second light sources (61, 62) and the photoelectric sensor from one another. a processor; a photoelectric detection circuit, configured to detect signals of the strength of light reflection in the detection zone and the blank zone and feedback detection information to the processor; wherein the photoelectric detection circuit comprises: at least two light sources (61, 62), corresponding to positions of the detection zone and the blank zone of the test strip, and able to emit lights corresponding to the detection zone and blank zone of the test strip; and at least one optical detector, configured to receive reflected lights from the detection zone and the blank zone; wherein the lights emitted by the at least two light sources (61, 62) irradiate the detection zone and blank zone of the strip and are reflected therefrom, and then received by the optical detector, which in turn feedback the detection information to the processor; the device further comprising a diaphragm separating the at least two light sources (61, 62) and the at least one optical detector from one another, characterized in that the optical detector is a photoelectric sensor, at least two light sources (61, 62), corresponding to positions of the detection zone and the blank zone of the test strip, and able to emit lights corresponding to the detection zone and blank zone of the test strip; and at least one optical detector, configured to receive reflected lights from the detection zone and the blank zone; wherein the lights emitted by the at least two light sources (61, 62) irradiate the detection zone and blank zone of the strip and are reflected therefrom, and then received by the optical detector, which in turn feedback the detection information to the processor; the device further comprising a diaphragm separating the at least two light sources (61, 62) and the at least one optical detector from one another, characterized in that the optical detector is a photoelectric sensor, the at least two light sources are comprised of a first light source and a second light source, wherein the lights emitted by the two light sources (61, 62) irradiate the detection zone and blank zone of the test strip, and then are received by the photoelectric sensor after reflection, and the analyzing and reading device further comprises a T-shaped diaphragm separating the first, second light sources (61, 62) and the photoelectric sensor from one another.