A dosimetry system for determining a presence/distribution of cross-linking agent in corneal tissue, comprising: one or more light sources configured to generate excitation light delivered to the corneal tissue treated with a the cross-linking agent, the excitation light causing the cross-linking agent to fluoresce by emitting an emission light at a plurality of emission wavelengths; an image capture system configured to employ hyperspectral imaging to capture an image of the corneal tissue in response to the delivery of the excitation light to the corneal tissue, wherein the image shows at least two emission wavelengths (λ 1, λ 2) emitted by the corneal tissue and each of the at least two of the emission wavelengths (λ 1, λ 2) can be identified from the image; and a controller configured to receive the image of the corneal tissue, the controller including one or more processors and computer-readable storage media, the one or more processors configured to execute program instructions stored on the computer-readable storage media to: identify and distinguish each of the at least two emission wavelengths (λ 1, λ 2) in the image; determine, from the image, respective characteristics associated separately with each of the at least two emission wavelengths (λ 1, λ 2); and provide information relating to cross-linking activity generated by the cross-linking agent in the corneal tissue based on the respective characteristics associated with each of the at least two emission wavelengths (λ 1, λ 2). one or more light sources configured to generate excitation light delivered to the corneal tissue treated with a the cross-linking agent, the excitation light causing the cross-linking agent to fluoresce by emitting an emission light at a plurality of emission wavelengths; an image capture system configured to employ hyperspectral imaging to capture an image of the corneal tissue in response to the delivery of the excitation light to the corneal tissue, wherein the image shows at least two emission wavelengths (λ 1, λ 2) emitted by the corneal tissue and each of the at least two of the emission wavelengths (λ 1, λ 2) can be identified from the image; and a controller configured to receive the image of the corneal tissue, the controller including one or more processors and computer-readable storage media, the one or more processors configured to execute program instructions stored on the computer-readable storage media to: identify and distinguish each of the at least two emission wavelengths (λ 1, λ 2) in the image; determine, from the image, respective characteristics associated separately with each of the at least two emission wavelengths (λ 1, λ 2); and provide information relating to cross-linking activity generated by the cross-linking agent in the corneal tissue based on the respective characteristics associated with each of the at least two emission wavelengths (λ 1, λ 2). identify and distinguish each of the at least two emission wavelengths (λ 1, λ 2) in the image; determine, from the image, respective characteristics associated separately with each of the at least two emission wavelengths (λ 1, λ 2); and provide information relating to cross-linking activity generated by the cross-linking agent in the corneal tissue based on the respective characteristics associated with each of the at least two emission wavelengths (λ 1, λ 2).