A method for measurement of α-fructosyl peptide in a sample, said method comprising allowing an amadoriase capable of reacting with one or more α-fructosyl peptides selected from among (a) to (e) below to react with a sample comprising one or more α-fructosyl peptides selected from among (a) to (e) and measuring the amount of hydrogen peroxide generated or oxygen consumed in such reaction: (a) α-fructosyl-valyl-histidyl-leucine (aF3P); (b) α-fructosyl-valyl-histidyl-leucyl-threonine (aF4P); (c) α-fructosyl-valyl-histidyl-leucyl-threonyl-proline (aF5P); (d) a-fructosyl-valyl-histidyl-leucyl-threonyl-proryl-glutamyl-glutamyl-lysine (aF8P); and (e) a-fructosyl-valyl-histidyl-leucyl-threonyl-proryl-glutamyl-glutamyl-lysyl-seryl-alanyl-valyl-threonyl-alanyl-leucyl-tryptophyl-glycine (αF16P), wherein the amadoriase is: (i) an amadoriase with the amino acid sequence shown in SEQ ID NO: 141, SEQ ID NO: 185, SEQ ID NO: 187 or SEQ ID NO: 189; or (ii) an amadoriase having 90% or higher sequence identity with the amino acid sequence shown in SEQ ID NO: 141 wherein said amadoriase has an amino acid substitution at each of amino acid positions corresponding to positions 62, 63, 102, 106, 110, 113 and 355 of the amino acid sequence of SEQ ID NO:1; (iii) an amadoriase having 90% or higher sequence identity with the amino acid sequence shown in SEQ ID NO: 185 wherein said amadoriase has an amino acid substitution at each of amino acid positions corresponding to positions 61, 62, 101, 105, 109, 112 and 355 of the amino acid sequence of SEQ ID NO:1; (iv) an amadoriase having 90% or higher sequence identity with the amino acid sequence shown in SEQ ID NO: 187 wherein said amadoriase has an amino acid substitution at each of amino acid positions corresponding to positions 62, 63, 106, 110, 113 and 351 of the amino acid sequence of SEQ ID NO:1; or (v) an amadoriase having 90% or higher sequence identity with the amino acid sequence shown in SEQ ID NO: 189 wherein said amadoriase has an amino acid substitution at each of amino acid positions corresponding to positions 62, 106, 110 and 113 of the amino acid sequence of SEQ ID NO:1; wherein said amadoriase is capable of reacting with one or more α-fructosyl peptides selected from among (a) to (e) above. (a) α-fructosyl-valyl-histidyl-leucine (aF3P); (b) α-fructosyl-valyl-histidyl-leucyl-threonine (aF4P); (c) α-fructosyl-valyl-histidyl-leucyl-threonyl-proline (aF5P); (d) a-fructosyl-valyl-histidyl-leucyl-threonyl-proryl-glutamyl-glutamyl-lysine (aF8P); and (e) a-fructosyl-valyl-histidyl-leucyl-threonyl-proryl-glutamyl-glutamyl-lysyl-seryl-alanyl-valyl-threonyl-alanyl-leucyl-tryptophyl-glycine (αF16P), wherein the amadoriase is: (i) an amadoriase with the amino acid sequence shown in SEQ ID NO: 141, SEQ ID NO: 185, SEQ ID NO: 187 or SEQ ID NO: 189; or (ii) an amadoriase having 90% or higher sequence identity with the amino acid sequence shown in SEQ ID NO: 141 wherein said amadoriase has an amino acid substitution at each of amino acid positions corresponding to positions 62, 63, 102, 106, 110, 113 and 355 of the amino acid sequence of SEQ ID NO:1; (iii) an amadoriase having 90% or higher sequence identity with the amino acid sequence shown in SEQ ID NO: 185 wherein said amadoriase has an amino acid substitution at each of amino acid positions corresponding to positions 61, 62, 101, 105, 109, 112 and 355 of the amino acid sequence of SEQ ID NO:1; (iv) an amadoriase having 90% or higher sequence identity with the amino acid sequence shown in SEQ ID NO: 187 wherein said amadoriase has an amino acid substitution at each of amino acid positions corresponding to positions 62, 63, 106, 110, 113 and 351 of the amino acid sequence of SEQ ID NO:1; or (v) an amadoriase having 90% or higher sequence identity with the amino acid sequence shown in SEQ ID NO: 189 wherein said amadoriase has an amino acid substitution at each of amino acid positions corresponding to positions 62, 106, 110 and 113 of the amino acid sequence of SEQ ID NO:1; wherein said amadoriase is capable of reacting with one or more α-fructosyl peptides selected from among (a) to (e) above. (i) an amadoriase with the amino acid sequence shown in SEQ ID NO: 141, SEQ ID NO: 185, SEQ ID NO: 187 or SEQ ID NO: 189; or (ii) an amadoriase having 90% or higher sequence identity with the amino acid sequence shown in SEQ ID NO: 141 wherein said amadoriase has an amino acid substitution at each of amino acid positions corresponding to positions 62, 63, 102, 106, 110, 113 and 355 of the amino acid sequence of SEQ ID NO:1; (iii) an amadoriase having 90% or higher sequence identity with the amino acid sequence shown in SEQ ID NO: 185 wherein said amadoriase has an amino acid substitution at each of amino acid positions corresponding to positions 61, 62, 101, 105, 109, 112 and 355 of the amino acid sequence of SEQ ID NO:1; (iv) an amadoriase having 90% or higher sequence identity with the amino acid sequence shown in SEQ ID NO: 187 wherein said amadoriase has an amino acid substitution at each of amino acid positions corresponding to positions 62, 63, 106, 110, 113 and 351 of the amino acid sequence of SEQ ID NO:1; or (v) an amadoriase having 90% or higher sequence identity with the amino acid sequence shown in SEQ ID NO: 189 wherein said amadoriase has an amino acid substitution at each of amino acid positions corresponding to positions 62, 106, 110 and 113 of the amino acid sequence of SEQ ID NO:1; wherein said amadoriase is capable of reacting with one or more α-fructosyl peptides selected from among (a) to (e) above.