An isolated nucleic acid molecule comprising a polynucleotide encoding a chimeric protein, the polynucleotide comprising: a synthetic Brassica -derived nucleotide sequence that encodes a single, chimeric peptide comprising a contiguous amino acid sequence of a first Brassica chloroplast transit peptide, the chimeric peptide further comprising a contiguous amino acid sequence of a second, different CTP, wherein the chimeric peptide comprises an amino acid sequence that is at least 90% identical to SEQ ID NO:3 or SEQ ID NO:4; and a nucleotide sequence encoding a polypeptide of interest operably linked to the synthetic Brassica -derived nucleotide sequence, wherein preferably the isolated nucleic acid molecule fulfills at least one of the following: (i) the polynucleotide is operably linked to one or more regulatory sequences; (ii) the first Brassica CTP is from Brassica napus or Brassica rapa, wherein preferably the second, different CTP is from a Brassica sp. other than the first Brassica CTP; (iii) the first Brassica CTP of the chimeric protein encoded by the polynucleotide is from an 3-enolpyruvylshikimate-5-phosphate synthetase (EPSPS) gene; (iv) the second, different CTP of the chimeric protein encoded by the polynucleotide is from an EPSPS gene; (v) the chimeric peptide encoded by the polynucleotide comprises an amino acid sequence that is at least 95%, preferably at least 98%, and most preferred 100% identical to SEQ ID NO:3 or SEQ ID NO:4; (vi) the polypeptide of interest of the chimeric protein encoded by the polynucleotide is not SEQ ID NO:1 or SEQ ID NO:2; and (vii) the nucleotide sequence that encodes the chimeric peptide is selected from the group consisting of SEQ ID NOs: 5, 6, 8, and 9. a synthetic Brassica -derived nucleotide sequence that encodes a single, chimeric peptide comprising a contiguous amino acid sequence of a first Brassica chloroplast transit peptide, the chimeric peptide further comprising a contiguous amino acid sequence of a second, different CTP, wherein the chimeric peptide comprises an amino acid sequence that is at least 90% identical to SEQ ID NO:3 or SEQ ID NO:4; and a nucleotide sequence encoding a polypeptide of interest operably linked to the synthetic Brassica -derived nucleotide sequence, wherein preferably the isolated nucleic acid molecule fulfills at least one of the following: (i) the polynucleotide is operably linked to one or more regulatory sequences; (ii) the first Brassica CTP is from Brassica napus or Brassica rapa, wherein preferably the second, different CTP is from a Brassica sp. other than the first Brassica CTP; (iii) the first Brassica CTP of the chimeric protein encoded by the polynucleotide is from an 3-enolpyruvylshikimate-5-phosphate synthetase (EPSPS) gene; (iv) the second, different CTP of the chimeric protein encoded by the polynucleotide is from an EPSPS gene; (v) the chimeric peptide encoded by the polynucleotide comprises an amino acid sequence that is at least 95%, preferably at least 98%, and most preferred 100% identical to SEQ ID NO:3 or SEQ ID NO:4; (vi) the polypeptide of interest of the chimeric protein encoded by the polynucleotide is not SEQ ID NO:1 or SEQ ID NO:2; and (vii) the nucleotide sequence that encodes the chimeric peptide is selected from the group consisting of SEQ ID NOs: 5, 6, 8, and 9. (i) the polynucleotide is operably linked to one or more regulatory sequences; (ii) the first Brassica CTP is from Brassica napus or Brassica rapa, wherein preferably the second, different CTP is from a Brassica sp. other than the first Brassica CTP; (iii) the first Brassica CTP of the chimeric protein encoded by the polynucleotide is from an 3-enolpyruvylshikimate-5-phosphate synthetase (EPSPS) gene; (iv) the second, different CTP of the chimeric protein encoded by the polynucleotide is from an EPSPS gene; (v) the chimeric peptide encoded by the polynucleotide comprises an amino acid sequence that is at least 95%, preferably at least 98%, and most preferred 100% identical to SEQ ID NO:3 or SEQ ID NO:4; (vi) the polypeptide of interest of the chimeric protein encoded by the polynucleotide is not SEQ ID NO:1 or SEQ ID NO:2; and (vii) the nucleotide sequence that encodes the chimeric peptide is selected from the group consisting of SEQ ID NOs: 5, 6, 8, and 9.