A method of detecting one or more nucleic acid targets within an individual cell, the method comprising: providing a sample comprising the cell, which cell comprises or is suspected of comprising one or more nucleic acid targets; fixing and permeabilizing the cell; for each nucleic acid target, providing (i) one or more label probes, wherein each label probe comprises one or more labels, (ii) one or more label probes, and one or more amplifiers, wherein each label probe comprises one or more labels, and wherein each amplifier is capable of hybridizing to one or more label probes, or (iii) one or more label probes, one or more amplifiers, and one or more preamplifiers, wherein each label probe comprises one or more labels, wherein each amplifier is capable of hybridizing to one or more label probes, and wherein each preamplifier is capable of hybridizing to one or more amplifiers; for each nucleic acid target, providing two or more different capture probes, wherein each of the two or more capture probes comprises a section T complementary to a section on the nucleic acid target and a section L complementary to a section on the label probe, or on an amplifier, or on a preamplifier, and wherein the T sections are complementary to nonoverlapping regions of the nucleic acid target, and the L sections are complementary to nonoverlapping regions of the label probe, the amplifier, or the preamplifier; hybridizing, in the cell, the two or more capture probes to a single copy of the nucleic acid target, when present in the cell; capturing the label probe to the two or more capture probes, thereby capturing the label probe to the nucleic acid target, by simultaneously hybridizing at least two different capture probes to a single copy of the label probe, or by simultaneously hybridizing at least two different capture probes to a single copy of the amplifier and hybridizing the label probes to the amplifier, or by simultaneously hybridizing at least two different capture probes to a single copy of the preamplifier and hybridizing the one or more amplifiers to the preamplifier and the one or more label probes to each of the one or more amplifiers; and detecting a signal from the label. providing a sample comprising the cell, which cell comprises or is suspected of comprising one or more nucleic acid targets; fixing and permeabilizing the cell; for each nucleic acid target, providing (i) one or more label probes, wherein each label probe comprises one or more labels, (ii) one or more label probes, and one or more amplifiers, wherein each label probe comprises one or more labels, and wherein each amplifier is capable of hybridizing to one or more label probes, or (iii) one or more label probes, one or more amplifiers, and one or more preamplifiers, wherein each label probe comprises one or more labels, wherein each amplifier is capable of hybridizing to one or more label probes, and wherein each preamplifier is capable of hybridizing to one or more amplifiers; (i) one or more label probes, wherein each label probe comprises one or more labels, (ii) one or more label probes, and one or more amplifiers, wherein each label probe comprises one or more labels, and wherein each amplifier is capable of hybridizing to one or more label probes, or (iii) one or more label probes, one or more amplifiers, and one or more preamplifiers, wherein each label probe comprises one or more labels, wherein each amplifier is capable of hybridizing to one or more label probes, and wherein each preamplifier is capable of hybridizing to one or more amplifiers; for each nucleic acid target, providing two or more different capture probes, wherein each of the two or more capture probes comprises a section T complementary to a section on the nucleic acid target and a section L complementary to a section on the label probe, or on an amplifier, or on a preamplifier, and wherein the T sections are complementary to nonoverlapping regions of the nucleic acid target, and the L sections are complementary to nonoverlapping regions of the label probe, the amplifier, or the preamplifier; hybridizing, in the cell, the two or more capture probes to a single copy of the nucleic acid target, when present in the cell; capturing the label probe to the two or more capture probes, thereby capturing the label probe to the nucleic acid target, by simultaneously hybridizing at least two different capture probes to a single copy of the label probe, or by simultaneously hybridizing at least two different capture probes to a single copy of the amplifier and hybridizing the label probes to the amplifier, or by simultaneously hybridizing at least two different capture probes to a single copy of the preamplifier and hybridizing the one or more amplifiers to the preamplifier and the one or more label probes to each of the one or more amplifiers; and detecting a signal from the label.