A manufacturing method of a microstructure, comprising: providing a patch manufacturing sheet formed in a state in which a bottom layer is exposed; providing the patch manufacturing sheet on a first process substrate and a second process substrate; spotting a viscous composition at a plurality of positions spaced apart from each other on the bottom layer with respect to only the patch manufacturing sheet provided on the first process substrate, or at a plurality of positions spaced apart from each other on each of the bottom layers with respect to both of the patch manufacturing sheets provided on the first process substrate and the patch manufacturing sheets provided on the second process substrate; contacting the patch manufacturing sheet provided on the second process substrate to the viscous composition spotted on the patch manufacturing sheet provided on the first process substrate, or contacting the viscous composition, which is spotted on the patch manufacturing sheet provided on the second process substrate, to the viscous composition spotted on the patch manufacturing sheet provided on the first process substrate; separating relatively a vertical direction distance between the first process substrate and the second process substrate to stretch the viscous composition and to coagulate the stretched viscous composition; and cutting the coagulated viscous composition, wherein the providing of the patch manufacturing sheet formed in a state in which the bottom layer is exposed includes: providing the patch manufacturing sheet including a supporting layer, an adhesive layer, and a peeling film; removing a portion of the peeling film of the patch manufacturing sheet to expose the adhesive layer; and attaching the bottom layer to the exposed adhesive layer, wherein the bottom layer is manufactured to correspond to a size and a shape of the exposed adhesive layer. providing a patch manufacturing sheet formed in a state in which a bottom layer is exposed; providing the patch manufacturing sheet on a first process substrate and a second process substrate; spotting a viscous composition at a plurality of positions spaced apart from each other on the bottom layer with respect to only the patch manufacturing sheet provided on the first process substrate, or at a plurality of positions spaced apart from each other on each of the bottom layers with respect to both of the patch manufacturing sheets provided on the first process substrate and the patch manufacturing sheets provided on the second process substrate; contacting the patch manufacturing sheet provided on the second process substrate to the viscous composition spotted on the patch manufacturing sheet provided on the first process substrate, or contacting the viscous composition, which is spotted on the patch manufacturing sheet provided on the second process substrate, to the viscous composition spotted on the patch manufacturing sheet provided on the first process substrate; separating relatively a vertical direction distance between the first process substrate and the second process substrate to stretch the viscous composition and to coagulate the stretched viscous composition; and cutting the coagulated viscous composition, wherein the providing of the patch manufacturing sheet formed in a state in which the bottom layer is exposed includes: providing the patch manufacturing sheet including a supporting layer, an adhesive layer, and a peeling film; removing a portion of the peeling film of the patch manufacturing sheet to expose the adhesive layer; and attaching the bottom layer to the exposed adhesive layer, wherein the bottom layer is manufactured to correspond to a size and a shape of the exposed adhesive layer. providing the patch manufacturing sheet including a supporting layer, an adhesive layer, and a peeling film; removing a portion of the peeling film of the patch manufacturing sheet to expose the adhesive layer; and attaching the bottom layer to the exposed adhesive layer, wherein the bottom layer is manufactured to correspond to a size and a shape of the exposed adhesive layer.