A centrifugation system comprising, in combination: a centrifuge configured to separate blood components from blood by rotation about a rotational axis and comprising a generally annular low-G wall, and a generally annular high-G wall located farther from the rotational axis than the low-G wall, wherein the high-G wall includes a first projection extending toward the low-G wall and the low-G wall includes a second projection extending toward the high-G wall; and a multiple-stage separation chamber having a top transverse edge and configured to be received in the centrifuge between the low-G and high-G walls (146, 144), comprising a first stage and a second stage, wherein the first stage of the separation chamber comprises an inlet port configured for flowing blood into the separation chamber, a plasma outlet port for flowing a separated blood component substantially comprising plasma out of the separation chamber, and a red cell outlet port for flowing a separated blood component substantially comprising red blood cells out of the separation chamber wherein the second stage comprises an inlet port and an outlet port, wherein the plasma outlet port and the inlet port of the second stage are coupled for transferring the separated blood component substantially comprising plasma from the first stage into the second stage, wherein the inlet ports (70, 72) and outlet ports (66, 68, 74) are arranged side-by-side along the top transverse edge of the separation chamber wherein, when the separation chamber is received in the centrifuge between the low-G and high-G walls (146, 144), the first projection is oriented so as to extend into the path of separated blood components flowing toward the plasma outlet port and prevent the separated blood component substantially comprising red blood cells from flowing into the plasma outlet port. wherein the second projection is oriented so as to extend into the path of separated blood components flowing toward the red cell outlet port and prevent the separated blood component substantially comprising plasma from flowing into the red cell outlet port, wherein the separation chamber includes a first interior seal positioned between the red cell outlet port and the plasma outlet port, and further includes a second interior seal located between the red cell outlet port and the inlet port of the first stage, wherein one end of the second projection is positioned at or adjacent to an end of the first interior seal and another end of the second projection is positioned at or adjacent to the second interior seal when the separation chamber is received in the centrifuge between the low-G and high-G walls (146, 144), and wherein one end of the first projection is substantially angularly aligned with one end of the second projection. a centrifuge configured to separate blood components from blood by rotation about a rotational axis and comprising a generally annular low-G wall, and a generally annular high-G wall located farther from the rotational axis than the low-G wall, wherein the high-G wall includes a first projection extending toward the low-G wall and the low-G wall includes a second projection extending toward the high-G wall; and a generally annular low-G wall, and a generally annular high-G wall located farther from the rotational axis than the low-G wall, wherein the high-G wall includes a first projection extending toward the low-G wall and the low-G wall includes a second projection extending toward the high-G wall; and a multiple-stage separation chamber having a top transverse edge and configured to be received in the centrifuge between the low-G and high-G walls (146, 144), comprising a first stage and a second stage, wherein the first stage of the separation chamber comprises an inlet port configured for flowing blood into the separation chamber, a plasma outlet port for flowing a separated blood component substantially comprising plasma out of the separation chamber, and a red cell outlet port for flowing a separated blood component substantially comprising red blood cells out of the separation chamber wherein the second stage comprises an inlet port and an outlet port, wherein the plasma outlet port and the inlet port of the second stage are coupled for transferring the separated blood component substantially comprising plasma from the first stage into the second stage, an inlet port configured for flowing blood into the separation chamber, a plasma outlet port for flowing a separated blood component substantially comprising plasma out of the separation chamber, and a red cell outlet port for flowing a separated blood component substantially comprising red blood cells out of the separation chamber wherein the second stage comprises an inlet port and an outlet port, wherein the plasma outlet port and the inlet port of the second stage are coupled for transferring the separated blood component substantially comprising plasma from the first stage into the second stage,