A device comprising: - a support comprising a support base defining - a spherical base contact surface extending along a virtual sphere having a radius between 10 and 15 mm to match an outside surface of an eye, and - a circular rim extending in a rim plane (P 20), having an axis X perpendicular to said rim plane (P 20) and a radius of greater than 5 mm and of less than 8 mm, so as to match the limbus of the eye; - a first needle and a second needle (8a;8b) having a first tip and a second tip (36a;36b), respectively, and being mobile on the support between - first and second extracted positions, in which the first and second needles are outside said virtual sphere, respectively, and - first and second inserted positions, in which the first and second needles are maximally inserted in the virtual sphere, respectively, via first and second intermediate piercing positions in which, respectively, the first and second needles extend along first and second insertion axis (Δ 8a;Δ 8b) and the first and second tips are located on first and second insertion points (M 8a;M 8b) belonging respectively to first and second hemispheres of the virtual sphere which are separated by a plane including the axis X and including a central point at mid-length of the rim, or "median radial plane of the rim" (Pm 20), an angle (θ) between the median radial plane of the rim and any of the first and second insertion axis being greater than 70° and less than 110°, said device further comprising - an electroporation generator comprising first and second terminals (9a, 9b) with different polarities, - - a plate electrode fixed on the support, connected to the second terminal, and defining a plate electrode contact surface which extends along the virtual sphere, the dimensions of the plate electrode contact surface being preferably such that the plate electrode contact surface substantially extends in a general "plate electrode plane", characterised in that an invasive electrode is electrically connected to the first terminal and comprises said first and second needles. - a support comprising a support base defining - a spherical base contact surface extending along a virtual sphere having a radius between 10 and 15 mm to match an outside surface of an eye, and - a circular rim extending in a rim plane (P 20), having an axis X perpendicular to said rim plane (P 20) and a radius of greater than 5 mm and of less than 8 mm, so as to match the limbus of the eye; - a spherical base contact surface extending along a virtual sphere having a radius between 10 and 15 mm to match an outside surface of an eye, and - a circular rim extending in a rim plane (P 20), having an axis X perpendicular to said rim plane (P 20) and a radius of greater than 5 mm and of less than 8 mm, so as to match the limbus of the eye; - a first needle and a second needle (8a;8b) having a first tip and a second tip (36a;36b), respectively, and being mobile on the support between - first and second extracted positions, in which the first and second needles are outside said virtual sphere, respectively, and - first and second inserted positions, in which the first and second needles are maximally inserted in the virtual sphere, respectively, via first and second intermediate piercing positions in which, respectively, the first and second needles extend along first and second insertion axis (Δ 8a;Δ 8b) and the first and second tips are located on first and second insertion points (M 8a;M 8b) belonging respectively to first and second hemispheres of the virtual sphere which are separated by a plane including the axis X and including a central point at mid-length of the rim, or "median radial plane of the rim" (Pm 20), an angle (θ) between the median radial plane of the rim and any of the first and second insertion axis being greater than 70° and less than 110°, said device further comprising - first and second extracted positions, in which the first and second needles are outside said virtual sphere, respectively, and - first and second inserted positions, in which the first and second needles are maximally inserted in the virtual sphere, respectively, via first and second intermediate piercing positions in which, respectively, the first and second needles extend along first and second insertion axis (Δ 8a;Δ 8b) and the first and second tips are located on first and second insertion points (M 8a;M 8b) belonging respectively to first and second hemispheres of the virtual sphere which are separated by a plane including the axis X and including a central point at mid-length of the rim, or "median radial plane of the rim" (Pm 20), an angle (θ) between the median radial plane of the rim and any of the first and second insertion axis being greater than 70° and less than 110°, said device further comprising - an electroporation generator comprising first and second terminals (9a, 9b) with different polarities, - - a plate electrode fixed on the support, connected to the second terminal, and defining a plate electrode contact surface which extends along the virtual sphere, the dimensions of the plate electrode contact surface being preferably such that the plate electrode contact surface substantially extends in a general "plate electrode plane",