A use of a nanoplasmonic platform for the detection and quantification of at least one biological moiety in a sample, the nanoplasmonic platform comprising: a substrate having an immobilization layer; a plurality of nanoparticles immobilized on the immobilization layer; and at least one recognition element linked to the nanoparticles, the at least one recognition element configured to selectively bind to the at least one biological moiety; wherein the nanoplasmonic platform is used for the detection and quantification of the at least one biological moiety, wherein the use comprises: receiving the sample on the nanoplasmonic platform to capture the biological moiety; performing localized surface plasmon resonance on the nanoplasmonic platform to obtain a wavelength signal corresponding to the sample received on the nanoplasmonic platform; acquire a wavelength shift and an extinction intensity from the obtained wavelength signal; correlating the acquired wavelength shift and correlating the acquired extinction intensity corresponding to the sample received on the nanoplasmonic platform to determine whether the biological moiety has bound to the at least one recognition element on the nanoplasmonic platform, or correlating the acquired wavelength shift and correlating the acquired extinction intensity corresponding to the sample received on the nanoplasmonic platform to determine a quantity of the biological moiety present in the sample; wherein the plurality of nanoparticles comprise gold, wherein the at least one biological moiety comprises a pathogen, and wherein the at least one recognition element comprises an antibody. a substrate having an immobilization layer; a plurality of nanoparticles immobilized on the immobilization layer; and at least one recognition element linked to the nanoparticles, the at least one recognition element configured to selectively bind to the at least one biological moiety; wherein the nanoplasmonic platform is used for the detection and quantification of the at least one biological moiety, wherein the use comprises: receiving the sample on the nanoplasmonic platform to capture the biological moiety; performing localized surface plasmon resonance on the nanoplasmonic platform to obtain a wavelength signal corresponding to the sample received on the nanoplasmonic platform; acquire a wavelength shift and an extinction intensity from the obtained wavelength signal; correlating the acquired wavelength shift and correlating the acquired extinction intensity corresponding to the sample received on the nanoplasmonic platform to determine whether the biological moiety has bound to the at least one recognition element on the nanoplasmonic platform, or correlating the acquired wavelength shift and correlating the acquired extinction intensity corresponding to the sample received on the nanoplasmonic platform to determine a quantity of the biological moiety present in the sample; wherein the plurality of nanoparticles comprise gold, wherein the at least one biological moiety comprises a pathogen, and wherein the at least one recognition element comprises an antibody. receiving the sample on the nanoplasmonic platform to capture the biological moiety; performing localized surface plasmon resonance on the nanoplasmonic platform to obtain a wavelength signal corresponding to the sample received on the nanoplasmonic platform; acquire a wavelength shift and an extinction intensity from the obtained wavelength signal; correlating the acquired wavelength shift and correlating the acquired extinction intensity corresponding to the sample received on the nanoplasmonic platform to determine whether the biological moiety has bound to the at least one recognition element on the nanoplasmonic platform, or correlating the acquired wavelength shift and correlating the acquired extinction intensity corresponding to the sample received on the nanoplasmonic platform to determine a quantity of the biological moiety present in the sample; wherein the plurality of nanoparticles comprise gold, wherein the at least one biological moiety comprises a pathogen, and wherein the at least one recognition element comprises an antibody.