A method of producing a rotavirus like particle in a plant, portion of a plant or plant cell comprising: a-i) providing the plant, portion of the plant or plant cell comprising one or more nucleic acid comprising a first nucleotide sequence encoding a first rotavirus protein, a second nucleotide sequence encoding a second rotavirus protein, a third nucleotide sequence encoding a third rotavirus protein, and a fourth nucleotide sequence encoding a fourth rotavirus protein, the first, second, third, and fourth nucleotide sequence being operatively linked to one or more regulatory region active in the plant, portion of the plant or plant cell; the first nucleotide sequence encoding rotavirus protein NSP4, the second nucleotide sequence encoding rotavirus structural protein VP6, the third nucleotide sequence encoding rotavirus structural protein VP2, and the fourth nucleotide sequence encoding rotavirus structural protein VP7; or a-ii) introducing into the plant, portion of the plant or plant cell by transformation the one or more nucleic acid comprising the first nucleotide sequence encoding the first rotavirus protein, the second nucleotide sequence encoding the second rotavirus protein, the third nucleotide sequence encoding the third rotavirus protein, and the fourth nucleotide sequence encoding the fourth rotavirus protein, the first, second, third and fourth nucleotide sequence being operatively linked to the one or more regulatory region active in the plant, portion of the plant or plant cell; and the first nucleotide sequence encoding the rotavirus protein NSP4, the second nucleotide sequence encoding the rotavirus structural protein VP6, the third nucleotide sequence encoding the rotavirus structural protein VP2, and the fourth nucleotide sequence encoding rotavirus structural protein VP7; and b) incubating the plant, portion of the plant or plant cell under conditions that permit the expression of the one or more nucleic acid, so that each of NSP4, VP6, VP2, and VP7 are expressed, thereby producing the RLP, the RLP comprising rotavirus proteins, the rotavirus proteins consisting of rotavirus structural proteins; and optionally c) harvesting the plant, portion of the plant or plant cell, and d) purifying the RLPs from the plant, portion of the plant or plant cell, wherein the RLPs range in size from 70-100 nm. a-i) providing the plant, portion of the plant or plant cell comprising one or more nucleic acid comprising a first nucleotide sequence encoding a first rotavirus protein, a second nucleotide sequence encoding a second rotavirus protein, a third nucleotide sequence encoding a third rotavirus protein, and a fourth nucleotide sequence encoding a fourth rotavirus protein, the first, second, third, and fourth nucleotide sequence being operatively linked to one or more regulatory region active in the plant, portion of the plant or plant cell; the first nucleotide sequence encoding rotavirus protein NSP4, the second nucleotide sequence encoding rotavirus structural protein VP6, the third nucleotide sequence encoding rotavirus structural protein VP2, and the fourth nucleotide sequence encoding rotavirus structural protein VP7; or a-ii) introducing into the plant, portion of the plant or plant cell by transformation the one or more nucleic acid comprising the first nucleotide sequence encoding the first rotavirus protein, the second nucleotide sequence encoding the second rotavirus protein, the third nucleotide sequence encoding the third rotavirus protein, and the fourth nucleotide sequence encoding the fourth rotavirus protein, the first, second, third and fourth nucleotide sequence being operatively linked to the one or more regulatory region active in the plant, portion of the plant or plant cell; and the first nucleotide sequence encoding the rotavirus protein NSP4, the second nucleotide sequence encoding the rotavirus structural protein VP6, the third nucleotide sequence encoding the rotavirus structural protein VP2, and the fourth nucleotide sequence encoding rotavirus structural protein VP7; and b) incubating the plant, portion of the plant or plant cell under conditions that permit the expression of the one or more nucleic acid, so that each of NSP4, VP6, VP2, and VP7 are expressed, thereby producing the RLP, the RLP comprising rotavirus proteins, the rotavirus proteins consisting of rotavirus structural proteins; and optionally c) harvesting the plant, portion of the plant or plant cell, and d) purifying the RLPs from the plant, portion of the plant or plant cell, wherein the RLPs range in size from 70-100 nm.