A bone marrow aspiration device comprising: an introducer needle including an introducer cannula having a proximal end and a distal end, each end including an opening, an introducer handle connected to the proximal end of the introducer cannula, and a removable introducer stylet having a proximal end and a distal end, the stylet extending through the introducer cannula from the cannula handle, the distal end of the stylet extending beyond the distal end of the introducer cannula and including a sharp tip to penetrate bone; an aspiration needle receivable in the introducer cannula when the introducer stylet is removed from the introducer needle, the aspiration needle including an aspiration cannula having a proximal end and a distal end, the proximal end including an opening and the distal end being closed, the aspiration cannula including an opening near the distal end of the aspiration cannula and forming a channel for aspirating bone marrow, and an aspiration handle connected to the proximal end of the aspiration cannula; wherein the aspiration device comprises: an adjustable depth guide coupled to the introducer handle, the depth guide configured to control depth of entry of the introducer cannula into bone and to provide for controlled aspiration during retrieval of the aspiration cannula from the bone marrow; wherein the aspiration needle includes a connector to couple to the introducer needle in an air-tight manner. an introducer needle including an introducer cannula having a proximal end and a distal end, each end including an opening, an introducer handle connected to the proximal end of the introducer cannula, and a removable introducer stylet having a proximal end and a distal end, the stylet extending through the introducer cannula from the cannula handle, the distal end of the stylet extending beyond the distal end of the introducer cannula and including a sharp tip to penetrate bone; an aspiration needle receivable in the introducer cannula when the introducer stylet is removed from the introducer needle, the aspiration needle including an aspiration cannula having a proximal end and a distal end, the proximal end including an opening and the distal end being closed, the aspiration cannula including an opening near the distal end of the aspiration cannula and forming a channel for aspirating bone marrow, and an aspiration handle connected to the proximal end of the aspiration cannula; wherein the aspiration device comprises: an adjustable depth guide coupled to the introducer handle, the depth guide configured to control depth of entry of the introducer cannula into bone and to provide for controlled aspiration during retrieval of the aspiration cannula from the bone marrow; wherein the aspiration needle includes a connector to couple to the introducer needle in an air-tight manner. an adjustable depth guide coupled to the introducer handle, the depth guide configured to control depth of entry of the introducer cannula into bone and to provide for controlled aspiration during retrieval of the aspiration cannula from the bone marrow; wherein the aspiration needle includes a connector to couple to the introducer needle in an air-tight manner.