A microfluidic reagent card, comprising: a card body, and a plurality of the micropipes fixed on the card body, wherein each of the plurality of micropipes is configured to have a sample inlet end and a closed end, and a detection liquid layer and a separation medium layer are arranged inside each of the plurality of micropipes in sequence from the sample inlet end to the closed end, wherein the separation medium layer is made of gel particles or glass beads, and each of the plurality of micropipes has a diameter of 0.1-1.0 mm; the sample inlet end of each of the plurality of micropipes is configured to be connected to an automatic quantitative sample distribution device, wherein the plurality of micropipes are adapted to be introduced a sample simultaneously; and the plurality of micropipes are arranged radially, the closed end of each of the plurality of micropipes is arranged at the lateral side of each of the plurality of micropipes, and the plurality of micropipes are radially distributed according to the center of centrifugation on a plane; each of the automatic quantitative sample distribution devices comprise a sample adding component, the sample adding component is connected to a quantitative region, the quantitative region is connected to the sample inlet of each of the plurality of micropipes, a closing portion is provided between the quantitative region and the sample inlet end of each of the plurality of micropipes, wherein the pathway between the quantitative region and the closing portion gradually narrows, the diameter of the pathway in front of the closing portion being 0.08 to 0.14 mm and the diameter of the pathway between the closing portion and the sample inlet end of each of the plurality of micropipes being 0.15 to 0.3 mm. a card body, and a plurality of the micropipes fixed on the card body, wherein each of the plurality of micropipes is configured to have a sample inlet end and a closed end, and a detection liquid layer and a separation medium layer are arranged inside each of the plurality of micropipes in sequence from the sample inlet end to the closed end, wherein the separation medium layer is made of gel particles or glass beads, and each of the plurality of micropipes has a diameter of 0.1-1.0 mm; the sample inlet end of each of the plurality of micropipes is configured to be connected to an automatic quantitative sample distribution device, wherein the plurality of micropipes are adapted to be introduced a sample simultaneously; and the plurality of micropipes are arranged radially, the closed end of each of the plurality of micropipes is arranged at the lateral side of each of the plurality of micropipes, and the plurality of micropipes are radially distributed according to the center of centrifugation on a plane; each of the automatic quantitative sample distribution devices comprise a sample adding component, the sample adding component is connected to a quantitative region, the quantitative region is connected to the sample inlet of each of the plurality of micropipes, a closing portion is provided between the quantitative region and the sample inlet end of each of the plurality of micropipes, wherein the pathway between the quantitative region and the closing portion gradually narrows, the diameter of the pathway in front of the closing portion being 0.08 to 0.14 mm and the diameter of the pathway between the closing portion and the sample inlet end of each of the plurality of micropipes being 0.15 to 0.3 mm.