A nucleic acid molecule comprising: a) two target binding domains AS and AS' that target binding of the nucleic acid molecule to the pre-mRNA of the dystrophin gene, wherein the two target binding domains AS and AS' are located respectively at the 5'-end and at the 3'-end of the nucleic acid molecule, b) a 3' splice region comprising a branch point, a polypyrimidine tract and a 3' splice acceptor site, c) a 5' splice region comprising a 5' splice donor site, d) a spacer sequence that separates the 3' splice region from the 5'-end target binding domain AS, e) a spacer sequence that separates the 5' splice region from the 3'-end target binding domain AS', and f) a nucleotide sequence to be trans -spliced to the target pre-mRNA, wherein said nucleotide sequence encodes at least a part of the dystrophin polypeptide, and is located between the 3' splice region and the 5' splice region of said nucleic acid, and wherein: • the 5'-end target binding domain AS targets the binding of the nucleic acid to the intron 22 of the pre-mRNA of the DMD gene; or • the 5'-end target binding domain AS comprises at least 20 successive nucleotides of one of the nucleotide sequence chosen among: SEQ ID NO 13 and SEQ ID NO 14; or • the 3'-end target binding domain AS' targets the binding of the nucleic acid to the intron 23 of the pre-RNA of the DMD gene; or • the 5'-end target binding domain AS targets the binding of the nucleic acid to the intron 22 of the pre-mRNA of the DMD gene and the 3'-end target binding domain AS' targets the binding of the nucleic acid to the intron 23 of the pre-RNA of the DMD gene; or • the 5'-end target binding domain AS comprises at least 20 successive nucleotides of one of the nucleotide sequence chosen among: SEQ ID NO 13 and SEQ ID NO 14 the 3'-end target binding domain AS' targets the binding of the nucleic acid to the intron 23 of the pre-RNA of the DMD gene. a) two target binding domains AS and AS' that target binding of the nucleic acid molecule to the pre-mRNA of the dystrophin gene, wherein the two target binding domains AS and AS' are located respectively at the 5'-end and at the 3'-end of the nucleic acid molecule, b) a 3' splice region comprising a branch point, a polypyrimidine tract and a 3' splice acceptor site, c) a 5' splice region comprising a 5' splice donor site, d) a spacer sequence that separates the 3' splice region from the 5'-end target binding domain AS, e) a spacer sequence that separates the 5' splice region from the 3'-end target binding domain AS', and f) a nucleotide sequence to be trans -spliced to the target pre-mRNA, wherein said nucleotide sequence encodes at least a part of the dystrophin polypeptide, and is located between the 3' splice region and the 5' splice region of said nucleic acid, and wherein: • the 5'-end target binding domain AS targets the binding of the nucleic acid to the intron 22 of the pre-mRNA of the DMD gene; or • the 5'-end target binding domain AS comprises at least 20 successive nucleotides of one of the nucleotide sequence chosen among: SEQ ID NO 13 and SEQ ID NO 14; or • the 3'-end target binding domain AS' targets the binding of the nucleic acid to the intron 23 of the pre-RNA of the DMD gene; or • the 5'-end target binding domain AS targets the binding of the nucleic acid to the intron 22 of the pre-mRNA of the DMD gene and the 3'-end target binding domain AS' targets the binding of the nucleic acid to the intron 23 of the pre-RNA of the DMD gene; or • the 5'-end target binding domain AS comprises at least 20 successive nucleotides of one of the nucleotide sequence chosen among: SEQ ID NO 13 and SEQ ID NO 14 the 3'-end target binding domain AS' targets the binding of the nucleic acid to the intron 23 of the pre-RNA of the DMD gene. • the 5'-end target binding domain AS targets the binding of the nucleic acid to the intron 22 of the pre-mRNA of the DMD gene; or • the 5'-end target binding domain AS comprises at least 20 successive nucleotides of one of the nucleotide sequence chosen among: SEQ ID NO 13 and SEQ ID NO 14; or • the 3'-end target binding domain AS' targets the binding of the nucleic acid to the intron 23 of the pre-RNA of the DMD gene; or • the 5'-end target binding domain AS targets the binding of the nucleic acid to the intron 22 of the pre-mRNA of the DMD gene and the 3'-end target binding domain AS' targets the binding of the nucleic acid to the intron 23 of the pre-RNA of the DMD gene; or • the 5'-end target binding domain AS comprises at least 20 successive nucleotides of one of the nucleotide sequence chosen among: SEQ ID NO 13 and SEQ ID NO 14 the 3'-end target binding domain AS' targets the binding of the nucleic acid to the intron 23 of the pre-RNA of the DMD gene.