A flow system for multiparameter data acquisition, particle sorting and enumeration, the system comprising: a fluidics system configured to propagate a fluid column within which particles of a sample move along a common sample path, wherein the sample is a test sample for measurement and analysis; a detection system for collecting one or more signals from each particle as it passes one or more detection stations (73, 156) along the common sample path, each signal being assigned a signal value to form a multiparameter data point for each particle, the detection system collecting during successive time intervals said multiparameter data points (208, 212, 214) in one or more predetermined subsets (114, 116, 118) of a data space, wherein the one or more predetermined subsets comprise one or more boundaries within the data space that encompass said multiparameter data points (208, 212, 214), to form a data profile for each of said time intervals, wherein the data profile corresponds to a number of multiparameter data points (208, 212, 214) counted within the one or more boundaries of each of the one or more predetermined subsets (114, 116, 118); and a control system operationally associated with the fluidics system and detection system, the control system determining a data profile characteristic of the current data profile and comparing the data profile characteristic either with that of a predetermined prior data profile or with a predetermined limit, wherein the control system is configured to actuate a corrective action in real time whenever said data profile characteristic exceeds the predetermined limit or differs from the data profile characteristic of the predetermined prior data profile. a fluidics system configured to propagate a fluid column within which particles of a sample move along a common sample path, wherein the sample is a test sample for measurement and analysis; a detection system for collecting one or more signals from each particle as it passes one or more detection stations (73, 156) along the common sample path, each signal being assigned a signal value to form a multiparameter data point for each particle, the detection system collecting during successive time intervals said multiparameter data points (208, 212, 214) in one or more predetermined subsets (114, 116, 118) of a data space, wherein the one or more predetermined subsets comprise one or more boundaries within the data space that encompass said multiparameter data points (208, 212, 214), to form a data profile for each of said time intervals, wherein the data profile corresponds to a number of multiparameter data points (208, 212, 214) counted within the one or more boundaries of each of the one or more predetermined subsets (114, 116, 118); and a control system operationally associated with the fluidics system and detection system, the control system determining a data profile characteristic of the current data profile and comparing the data profile characteristic either with that of a predetermined prior data profile or with a predetermined limit, wherein the control system is configured to actuate a corrective action in real time whenever said data profile characteristic exceeds the predetermined limit or differs from the data profile characteristic of the predetermined prior data profile.