An automated method for detecting at least two targets in the same sample on a single solid substrate, said method comprising: a. contacting the sample with at least a first exogenous proteinaceous binding entity in a manner resulting in deposition of the first PBE in proximity to its target; b. contacting the sample with reagents in a manner resulting in specific deposition of a detectable moiety in proximity to the first PBE; and c. treating the sample to reduce the ability of the first PBE to be further detected in the sample by contacting the sample with a volume of solution comprising a citrate buffer with a pH from 5 to 7 and contacting the solid substrate with a heat source, the volume of solution covers the sample at a volume to surface area ratio from 5μl/cm 2 to 500μl/cm 2, the solution comprising the citrate buffer is at a starting buffer temperature which is below the boiling point of the solution comprising the citrate buffer; and heating the heat source from a starting heat source temperature to at least a target heat source temperature over a length of time, the solution comprising the citrate buffer is raised from the starting buffer temperature to a second buffer temperature, the target heat source temperature and the second buffer temperature are below the boiling point of the solution comprising the citrate buffer; d. repeating (a) and (b) with at least a second PBE; and e. repeating (c) between each repeat of (a) and (b); wherein after (d), each detectable moiety is capable of being detected in the sample at a location that corresponds to the location of the PBE 's target. a. contacting the sample with at least a first exogenous proteinaceous binding entity in a manner resulting in deposition of the first PBE in proximity to its target; b. contacting the sample with reagents in a manner resulting in specific deposition of a detectable moiety in proximity to the first PBE; and c. treating the sample to reduce the ability of the first PBE to be further detected in the sample by contacting the sample with a volume of solution comprising a citrate buffer with a pH from 5 to 7 and contacting the solid substrate with a heat source, the volume of solution covers the sample at a volume to surface area ratio from 5μl/cm 2 to 500μl/cm 2, the solution comprising the citrate buffer is at a starting buffer temperature which is below the boiling point of the solution comprising the citrate buffer; and heating the heat source from a starting heat source temperature to at least a target heat source temperature over a length of time, the solution comprising the citrate buffer is raised from the starting buffer temperature to a second buffer temperature, the target heat source temperature and the second buffer temperature are below the boiling point of the solution comprising the citrate buffer; contacting the sample with a volume of solution comprising a citrate buffer with a pH from 5 to 7 and contacting the solid substrate with a heat source, the volume of solution covers the sample at a volume to surface area ratio from 5μl/cm 2 to 500μl/cm 2, the solution comprising the citrate buffer is at a starting buffer temperature which is below the boiling point of the solution comprising the citrate buffer; and heating the heat source from a starting heat source temperature to at least a target heat source temperature over a length of time, the solution comprising the citrate buffer is raised from the starting buffer temperature to a second buffer temperature, the target heat source temperature and the second buffer temperature are below the boiling point of the solution comprising the citrate buffer; d. repeating (a) and (b) with at least a second PBE; and e. repeating (c) between each repeat of (a) and (b);