An enzyme detection device for detecting the presence in a test sample of cleavage activity of an enzyme capable of cleaving a substrate, the device comprising: (i) an indicator molecule for adding to the test sample, said indicator molecule comprising (a) a cleavage site, which can be cleaved by said enzyme if said enzyme cleavage activity is present; (b) a first capture site; and (c) a detection region comprising a second capture site, wherein cleavage of the cleavage site results in release of a fragment of the indicator molecule comprising the detection region; (ii) a first capture zone to receive the test sample, wherein the first capture zone comprises first capture molecules capable of binding to the first capture site of the indicator molecule but not the cleaved fragment of the indicator molecule comprising the detection region and wherein the indicator molecule is captured by binding of the first capture site to the first capture molecules thereby substantially preventing any subsequent cleavage of the cleavage site by the enzyme; and (iii) a second capture zone to receive the test sample following contact of the test sample with the first capture zone, wherein the second capture zone comprises second capture molecules capable of binding to the second capture site of the indicator molecule, wherein the second capture zone is spatially separated from the first capture zone, and wherein detection of any cleaved indicator molecule via the detection region and bound via the second capture site to the second capture molecules, indicates the presence of cleavage activity of the enzyme in the test sample. (i) an indicator molecule for adding to the test sample, said indicator molecule comprising (a) a cleavage site, which can be cleaved by said enzyme if said enzyme cleavage activity is present; (b) a first capture site; and (c) a detection region comprising a second capture site, wherein cleavage of the cleavage site results in release of a fragment of the indicator molecule comprising the detection region; (a) a cleavage site, which can be cleaved by said enzyme if said enzyme cleavage activity is present; (b) a first capture site; and (c) a detection region comprising a second capture site, (ii) a first capture zone to receive the test sample, wherein the first capture zone comprises first capture molecules capable of binding to the first capture site of the indicator molecule but not the cleaved fragment of the indicator molecule comprising the detection region and wherein the indicator molecule is captured by binding of the first capture site to the first capture molecules thereby substantially preventing any subsequent cleavage of the cleavage site by the enzyme; and (iii) a second capture zone to receive the test sample following contact of the test sample with the first capture zone, wherein the second capture zone comprises second capture molecules capable of binding to the second capture site of the indicator molecule, wherein the second capture zone is spatially separated from the first capture zone, and wherein detection of any cleaved indicator molecule via the detection region and bound via the second capture site to the second capture molecules, indicates the presence of cleavage activity of the enzyme in the test sample.