A fluorescence imaging device for developing a fluorescence image of a selected tissue of a subject, the fluorescence imaging device comprising: a probe defining a central bore, the probe having an outer surface, a distal end, an opposed proximal end, and a longitudinal axis extending between the distal end and the proximal end of the probe, the distal end defining a distal tip, the probe comprising: image transmission means positioned in the central bore of the probe and configured for communication with a light source; and an objective lens positioned in the central bore of the probe proximate the distal tip of the probe; and a dye carrier comprising at least one fluorescent dye, the dye carrier having a bottom surface configured to contact the selected tissue of the subject, the dye carrier coupled to at least a portion of the outer surface of the probe such that the bottom surface of the dye carrier (a) extends beyond the distal tip of the probe such that the dye carrier can contact the selected tissue without the distal tip of the probe contacting the selected tissue, or (b) is substantially flush with the distal tip of the probe; the dye carrier being configured to selectively dispense the at least one fluorescent dye into the selected tissue of the subject, characterized in that the dye carrier is radially spaced from the central bore of the probe such that the dye carrier does not interfere with light transmission between the objective lens and the selected tissue. a probe defining a central bore, the probe having an outer surface, a distal end, an opposed proximal end, and a longitudinal axis extending between the distal end and the proximal end of the probe, the distal end defining a distal tip, the probe comprising: image transmission means positioned in the central bore of the probe and configured for communication with a light source; and an objective lens positioned in the central bore of the probe proximate the distal tip of the probe; and image transmission means positioned in the central bore of the probe and configured for communication with a light source; and an objective lens positioned in the central bore of the probe proximate the distal tip of the probe; and a dye carrier comprising at least one fluorescent dye, the dye carrier having a bottom surface configured to contact the selected tissue of the subject, the dye carrier coupled to at least a portion of the outer surface of the probe such that the bottom surface of the dye carrier (a) extends beyond the distal tip of the probe such that the dye carrier can contact the selected tissue without the distal tip of the probe contacting the selected tissue, or (b) is substantially flush with the distal tip of the probe; (a) extends beyond the distal tip of the probe such that the dye carrier can contact the selected tissue without the distal tip of the probe contacting the selected tissue, or (b) is substantially flush with the distal tip of the probe; the dye carrier being configured to selectively dispense the at least one fluorescent dye into the selected tissue of the subject, characterized in that the dye carrier is radially spaced from the central bore of the probe such that the dye carrier does not interfere with light transmission between the objective lens and the selected tissue.