Confocal microscopy apparatus (100; 200), comprising: - a structured light generating component (2; 13) configured to be illuminated with a basic light beam and to generate a structured light beam focused on a first plane; - a spinning disk configured to receive said structured light beam and to transmit a resulting excitation beam to an optics (8, 9) of a microscope focused on a plane of a sample, wherein the spinning disk lies on a second plane and comprises a disk-shaped substrate composed of an optically transparent material, the substrate of the spinning disk comprising a planar first surface and an opposing planar second surface and a patterned mask disposed on one of the first surface and the second surface and comprising at least one sector provided with one or more continuous spiral slit apertures, wherein the patterned mask or an outer surface thereof is composed of a highly black material opaque to light; - a housing configured to house on a third plane an acquisition sensor configured to detect a fluorescent beam emitted from said plane of the sample; - a set of relay lenses (4a, 4b, 4c) configured to optically conjugate the first plane, the second plane and said plane of the sample to the third plane; - optical means (5, 6) configured to transmit said structured light beam from the structured light generating component (2; 13) to said plane of the sample and said emitted fluorescent beam from said plane of the sample to said housing configured to house the acquisition sensor; and - moving means configured to move the structured light generating component (2; 13), so as to shift the structured light beam in the first plane, and the spinning disk in the second plane. - a structured light generating component (2; 13) configured to be illuminated with a basic light beam and to generate a structured light beam focused on a first plane; - a spinning disk configured to receive said structured light beam and to transmit a resulting excitation beam to an optics (8, 9) of a microscope focused on a plane of a sample, wherein the spinning disk lies on a second plane and comprises a disk-shaped substrate composed of an optically transparent material, the substrate of the spinning disk comprising a planar first surface and an opposing planar second surface and a patterned mask disposed on one of the first surface and the second surface and comprising at least one sector provided with one or more continuous spiral slit apertures, wherein the patterned mask or an outer surface thereof is composed of a highly black material opaque to light; - a housing configured to house on a third plane an acquisition sensor configured to detect a fluorescent beam emitted from said plane of the sample; - a set of relay lenses (4a, 4b, 4c) configured to optically conjugate the first plane, the second plane and said plane of the sample to the third plane; - optical means (5, 6) configured to transmit said structured light beam from the structured light generating component (2; 13) to said plane of the sample and said emitted fluorescent beam from said plane of the sample to said housing configured to house the acquisition sensor; and - moving means configured to move the structured light generating component (2; 13), so as to shift the structured light beam in the first plane, and the spinning disk in the second plane.