A method for making a type I sulfatase in an active form, the method comprising: a) providing a fungal cell genetically engineered to produce a protein with the type I sulfatase activating activity of a Formylglycine Generating Enzyme; and b) introducing into the cell a nucleic acid encoding a type I sulfatase, wherein the encoded type I sulfatase, without an activation step, is in an inactive form, wherein, after the introduction, the cell produces, or produces at an increased level, the type I sulfatase in an active form, and wherein, after step (b), the cell, or the progeny thereof, is cultivated at a pO 2 of 5% - 40%; or the method comprising: a) providing a fungal cell genetically engineered to produce a type I sulfatase, wherein the type I sulfatase, without an activation step, is in an inactive form; and b) introducing into the cell a nucleic acid encoding a protein with the type I sulfatase activating activity of a Formylglycine Generating Enzyme, wherein, after the introduction, the cell produces, or produces at an increased level, the type I sulfatase in an active form, and wherein, after step (b), the cell, or the progeny thereof, is cultivated at a pO 2 of 5% - 40%. the method comprising: a) providing a fungal cell genetically engineered to produce a protein with the type I sulfatase activating activity of a Formylglycine Generating Enzyme; and b) introducing into the cell a nucleic acid encoding a type I sulfatase, wherein the encoded type I sulfatase, without an activation step, is in an inactive form, wherein, after the introduction, the cell produces, or produces at an increased level, the type I sulfatase in an active form, and wherein, after step (b), the cell, or the progeny thereof, is cultivated at a pO 2 of 5% - 40%; or a) providing a fungal cell genetically engineered to produce a protein with the type I sulfatase activating activity of a Formylglycine Generating Enzyme; and b) introducing into the cell a nucleic acid encoding a type I sulfatase, wherein the encoded type I sulfatase, without an activation step, is in an inactive form, wherein, after the introduction, the cell produces, or produces at an increased level, the type I sulfatase in an active form, and wherein, after step (b), the cell, or the progeny thereof, is cultivated at a pO 2 of 5% - 40%; or the method comprising: a) providing a fungal cell genetically engineered to produce a type I sulfatase, wherein the type I sulfatase, without an activation step, is in an inactive form; and b) introducing into the cell a nucleic acid encoding a protein with the type I sulfatase activating activity of a Formylglycine Generating Enzyme, wherein, after the introduction, the cell produces, or produces at an increased level, the type I sulfatase in an active form, and wherein, after step (b), the cell, or the progeny thereof, is cultivated at a pO 2 of 5% - 40%. a) providing a fungal cell genetically engineered to produce a type I sulfatase, wherein the type I sulfatase, without an activation step, is in an inactive form; and b) introducing into the cell a nucleic acid encoding a protein with the type I sulfatase activating activity of a Formylglycine Generating Enzyme, wherein, after the introduction, the cell produces, or produces at an increased level, the type I sulfatase in an active form, and wherein, after step (b), the cell, or the progeny thereof, is cultivated at a pO 2 of 5% - 40%.