Method of neighbor influence compensation between a plurality of objects in at least one digital image, the at least one digital image containing image information about a plurality of objects, each of the plurality of objects being configured to receive at least one molecule comprising genetic information, the at least one molecule being configured to receive a fluorescent compound, the at least one digital image being taken by an optical imaging system during emission of electromagnetic radiation of the fluorescent compounds received by the at least one molecules, wherein the method comprises the following steps, preferably in the following order: a) providing a model describing the imaging of the objects by the optical system, which model defines equations, the equations relating determined intensities of the electromagnetic radiation having traversed the optical system with original intensities of the electromagnetic radiation; b) determining intensity values of the plurality of objects on the basis of the at least one digital image; and c) determining compensated intensity values of the plurality of objects on the basis of the determined intensity values and the model by solving the equations for the original intensities, wherein the digital image is partitioned into domains, each domain containing one object; wherein the model describes the determined intensities in terms of original intensities and weights; wherein a separate weight is associated to each pair of objects and domains; and wherein the model is only described by weights, where the distance between the object and the domain associated to the weight is below a predetermined cut-off distance. the at least one digital image containing image information about a plurality of objects, each of the plurality of objects being configured to receive at least one molecule comprising genetic information, the at least one molecule being configured to receive a fluorescent compound, the at least one digital image being taken by an optical imaging system during emission of electromagnetic radiation of the fluorescent compounds received by the at least one molecules, wherein the method comprises the following steps, preferably in the following order: a) providing a model describing the imaging of the objects by the optical system, which model defines equations, the equations relating determined intensities of the electromagnetic radiation having traversed the optical system with original intensities of the electromagnetic radiation; b) determining intensity values of the plurality of objects on the basis of the at least one digital image; and c) determining compensated intensity values of the plurality of objects on the basis of the determined intensity values and the model by solving the equations for the original intensities, a) providing a model describing the imaging of the objects by the optical system, which model defines equations, the equations relating determined intensities of the electromagnetic radiation having traversed the optical system with original intensities of the electromagnetic radiation; b) determining intensity values of the plurality of objects on the basis of the at least one digital image; and c) determining compensated intensity values of the plurality of objects on the basis of the determined intensity values and the model by solving the equations for the original intensities, wherein the digital image is partitioned into domains, each domain containing one object; wherein the model describes the determined intensities in terms of original intensities and weights; wherein a separate weight is associated to each pair of objects and domains; and wherein the model is only described by weights, where the distance between the object and the domain associated to the weight is below a predetermined cut-off distance.