A method for reconstructing absorption and/or scattering properties of a medium, using a reconstruction system comprising at least one radiation source (14, s) capable of illuminating a medium (12, MR, MC) and at least one detector (16, d) capable of receiving a signal emitted by the medium (12, MR, MC), the method being characterized in that it comprises the following steps: - establishing, for at least one source-detector pair (14, s, 16, d), a first distribution (A sd (t); A sd; A sd (ω)) of a signal received by the corresponding detector (16, d) for a reference medium, the received signal being emitted by the reference medium subsequent to the illumination of said medium by the corresponding source (14, s), - determining, for said at least one source-detector pair (14, s, 16, d), a first modelling function (G sd 0 (t); G sd 0; G sd 0 (ω)) of a light scattering signal between the source (14, s) and the detector (16, d) in the reference medium, - establishing, for said at least one source-detector pair (14, s, 16, d), a second distribution (B sd (t); B sd; B sd (ω)) of a signal received by the corresponding detector (16, d) for a medium to be characterized, the received signal being emitted by the medium to be characterized subsequent to the illumination of said medium by the corresponding source (14, s), - determining, for said at least one source-detector pair (14, s, 16, d), a second modelling function (G sd (t)) of a light scattering signal between the source (14, s) and the detector (16, d) in the medium to be characterized, and - computing a corrected signal (Y sd (t); Y sd; Y sd (ω)) as a function of the first modelling function (G sd 0 (t); G sd 0; G sd 0 (ω)), of the second modelling function (G sd (t); G sd; G sd (ω)), of the first distribution (A sd (t); A sd; A sd (ω)) and of the second distribution (B sd (t); B sd, B sd (ω)), the computing step including a comparison operation of the product of the first distribution (A sd (t); A sd; A sd (ω)) and the second modelling function (G sd (t); G sd; G sd (ω)) with the product of the second distribution (B sd (t); B s; B sd (ω)) and the first modelling function (G sd 0 (t); G sd 0; G sd 0 (ω)). - establishing, for at least one source-detector pair (14, s, 16, d), a first distribution (A sd (t); A sd; A sd (ω)) of a signal received by the corresponding detector (16, d) for a reference medium, the received signal being emitted by the reference medium subsequent to the illumination of said medium by the corresponding source (14, s), - determining, for said at least one source-detector pair (14, s, 16, d), a first modelling function (G sd 0 (t); G sd 0; G sd 0 (ω)) of a light scattering signal between the source (14, s) and the detector (16, d) in the reference medium, - establishing, for said at least one source-detector pair (14, s, 16, d), a second distribution (B sd (t); B sd; B sd (ω)) of a signal received by the corresponding detector (16, d) for a medium to be characterized, the received signal being emitted by the medium to be characterized subsequent to the illumination of said medium by the corresponding source (14, s), - determining, for said at least one source-detector pair (14, s, 16, d), a second modelling function (G sd (t)) of a light scattering signal between the source (14, s) and the detector (16, d) in the medium to be characterized, and - computing a corrected signal (Y sd (t); Y sd; Y sd (ω)) as a function of the first modelling function (G sd 0 (t); G sd 0; G sd 0 (ω)), of the second modelling function (G sd (t); G sd; G sd (ω)), of the first distribution (A sd (t); A sd; A sd (ω)) and of the second distribution (B sd (t); B sd, B sd (ω)),