Use of X-glucuronide, B-galactose and D-Glucose for increasing the intracellular concentration of at least on fluorescent marker molecule selected from the group consisting of Fluorescein, Fluorescein di(β-D-glucuronide), NileRed, and CY5 in Coliform bacteria in a method for rapid detection and quantification of live and recovered Coliform bacteria in a sample, comprising steps of: a. providing a detection cocktail comprising i. a nutrient medium comprising at least one of a mammalian host-derived fraction, for accelerated selective growing and multiplying of said microorganism; ii. at least one fluorescent marker molecule for detection of intracellular metabolism by a sensor selected from the group consisting of Fluorescein, Fluorescein di(β-D-glucuronide), NileRed, and CY5; iii. at least one metabolic activator selected from the group consisting of X-glucuronide, B-galactose and D-Glucose for specifically increasing metabolism of said Coliform bacteria and increasing intracellular concentration of said fluorescent marker molecule into said Coliform bacteria; b. contacting said sample with said detection cocktail; wherein said detection is microscopic detection and said quantification is by steps of: i. measuring gray levels of fluorescent intensity of said marker molecules by said sensor, said gray levels correlated to metabolism level of said live and recovered Coliform bacteria, ii. determining said gray level to a predetermined threshold of metabolism; further correlating said gray levels above said predetermined threshold with quantity of high metabolic-active said Coliform bacteria in said sample. a. providing a detection cocktail comprising i. a nutrient medium comprising at least one of a mammalian host-derived fraction, for accelerated selective growing and multiplying of said microorganism; ii. at least one fluorescent marker molecule for detection of intracellular metabolism by a sensor selected from the group consisting of Fluorescein, Fluorescein di(β-D-glucuronide), NileRed, and CY5; iii. at least one metabolic activator selected from the group consisting of X-glucuronide, B-galactose and D-Glucose for specifically increasing metabolism of said Coliform bacteria and increasing intracellular concentration of said fluorescent marker molecule into said Coliform bacteria; i. a nutrient medium comprising at least one of a mammalian host-derived fraction, for accelerated selective growing and multiplying of said microorganism; ii. at least one fluorescent marker molecule for detection of intracellular metabolism by a sensor selected from the group consisting of Fluorescein, Fluorescein di(β-D-glucuronide), NileRed, and CY5; iii. at least one metabolic activator selected from the group consisting of X-glucuronide, B-galactose and D-Glucose for specifically increasing metabolism of said Coliform bacteria and increasing intracellular concentration of said fluorescent marker molecule into said Coliform bacteria; b. contacting said sample with said detection cocktail; wherein said detection is microscopic detection and said quantification is by steps of: i. measuring gray levels of fluorescent intensity of said marker molecules by said sensor, said gray levels correlated to metabolism level of said live and recovered Coliform bacteria, ii. determining said gray level to a predetermined threshold of metabolism; further correlating said gray levels above said predetermined threshold with quantity of high metabolic-active said Coliform bacteria in said sample. i. measuring gray levels of fluorescent intensity of said marker molecules by said sensor, said gray levels correlated to metabolism level of said live and recovered Coliform bacteria, ii. determining said gray level to a predetermined threshold of metabolism;