A method of detecting a first analyte of interest and a second analyte of interest present in a sample at different concentrations, the method comprising: providing a lateral flow assay comprising a first complex coupled to a flow path of the lateral flow assay, the first complex comprising a label, an antibody or a fragment thereof that specifically binds the first analyte, and the first analyte, a labeled second antibody or fragment thereof coupled to the flow path and configured to specifically bind the second analyte, a first capture zone downstream of the first complex, the first capture zone comprising a first immobilized capture agent specific to the first analyte, and a second capture zone downstream of the labeled second antibody or fragment thereof and comprising a second immobilized capture agent specific to the second analyte; applying the sample to the first complex and the labeled second antibody or fragment thereof; binding the second analyte to the labeled second antibody or fragment thereof to form a second complex; flowing the fluid sample and the first complex to the first capture zone, where the first analyte in the fluid sample and the first complex compete to bind to the first immobilized capture agent in the first capture zone; flowing the second complex in the flow path to the second capture zone and binding the second complex to the second immobilized capture agent in the second capture zone; and detecting a first signal from the first complex bound to the first immobilized capture agent in the first capture zone and a second signal from the second complex bound to the second immobilized capture agent in the second capture zone. providing a lateral flow assay comprising a first complex coupled to a flow path of the lateral flow assay, the first complex comprising a label, an antibody or a fragment thereof that specifically binds the first analyte, and the first analyte, a labeled second antibody or fragment thereof coupled to the flow path and configured to specifically bind the second analyte, a first capture zone downstream of the first complex, the first capture zone comprising a first immobilized capture agent specific to the first analyte, and a second capture zone downstream of the labeled second antibody or fragment thereof and comprising a second immobilized capture agent specific to the second analyte; a first complex coupled to a flow path of the lateral flow assay, the first complex comprising a label, an antibody or a fragment thereof that specifically binds the first analyte, and the first analyte, a labeled second antibody or fragment thereof coupled to the flow path and configured to specifically bind the second analyte, a first capture zone downstream of the first complex, the first capture zone comprising a first immobilized capture agent specific to the first analyte, and a second capture zone downstream of the labeled second antibody or fragment thereof and comprising a second immobilized capture agent specific to the second analyte; applying the sample to the first complex and the labeled second antibody or fragment thereof; binding the second analyte to the labeled second antibody or fragment thereof to form a second complex; flowing the fluid sample and the first complex to the first capture zone, where the first analyte in the fluid sample and the first complex compete to bind to the first immobilized capture agent in the first capture zone; flowing the second complex in the flow path to the second capture zone and binding the second complex to the second immobilized capture agent in the second capture zone; and detecting a first signal from the first complex bound to the first immobilized capture agent in the first capture zone and a second signal from the second complex bound to the second immobilized capture agent in the second capture zone.