An image acquisition device, comprising: a stage on which a sample is placed; a light emitting means for emitting instantaneous light; a light guiding optical system including an objective lens arranged to face the sample on the stage; an imaging element for capturing an optical image of the sample guided by the light guiding optical system; a driving unit for moving a position of a field of view of the objective lens relative to the sample at a predetermined velocity; and a control unit for controlling the light emitting means, wherein the imaging element is a two-dimensional imaging element including a plurality of pixel rows and for sequentially capturing at a predetermined frame rate, and the predetermined velocity is a velocity set based on an optical magnification of the light guiding optical system, a pixel width of the pixel row of the imaging element, the number of pixel rows of the imaging element and the frame rate of the imaging element, wherein the apparatus is configured such that the movement of the position of the field of view of the objective lens and imaging of the imaging element are synchronized with each other so that a timing at which a field of view of the objective lens constitutes the position of each divided region matches a timing at which the imaging element performs imaging, wherein the driving unit is configured such that it moves the position of the field of view of the objective lens so that portions of two regions of the sample to be continuously captured by the imaging element overlap each other, wherein 2. the device is configured such that the velocity is a velocity set based on the number of pixel rows of the imaging element corresponding to an overlapping region in which portions of the two regions overlap each other, wherein the device is configured such that the overlapping is performed based on the number of pixel rows on the light reception surface of the imaging element corresponding to the overlapping region. a stage on which a sample is placed; a light emitting means for emitting instantaneous light; a light guiding optical system including an objective lens arranged to face the sample on the stage; an imaging element for capturing an optical image of the sample guided by the light guiding optical system; a driving unit for moving a position of a field of view of the objective lens relative to the sample at a predetermined velocity; and a control unit for controlling the light emitting means, wherein the imaging element is a two-dimensional imaging element including a plurality of pixel rows and for sequentially capturing at a predetermined frame rate, and the predetermined velocity is a velocity set based on an optical magnification of the light guiding optical system, a pixel width of the pixel row of the imaging element, the number of pixel rows of the imaging element and the frame rate of the imaging element, wherein the apparatus is configured such that the movement of the position of the field of view of the objective lens and imaging of the imaging element are synchronized with each other so that a timing at which a field of view of the objective lens constitutes the position of each divided region matches a timing at which the imaging element performs imaging, wherein the driving unit is configured such that it moves the position of the field of view of the objective lens so that portions of two regions of the sample to be continuously captured by the imaging element overlap each other, wherein 2. the device is configured such that the velocity is a velocity set based on the number of pixel rows of the imaging element corresponding to an overlapping region in which portions of the two regions overlap each other, wherein the device is configured such that the overlapping is performed based on the number of pixel rows on the light reception surface of the imaging element corresponding to the overlapping region.