A system for analyzing one or more molecules of interest in a liquid specimen, the system comprising: a fractionation system comprising: a pumping system; a detector having at least one sensor arranged relative to a multichannel flow pathway; a monolithic body defining a plurality of fractionation columns, each fractionation column comprising: an inlet chamber at a proximal end of the fractionation column; an outlet chamber at a distal, opposite end of the fractionation column; and a solid phase chamber extending between the inlet chamber and the outlet chamber packed with a metered amount of solid phase between a first porous frit and a second porous frit, wherein the inlet chambers of each of the plurality of fractionation columns are configured to be in fluid communication with the pumping system via proximal tubing, and wherein the outlet chambers of each of the plurality of fractionation columns are configured to direct eluate from the solid phase chamber toward the multichannel flow pathway of the detector via distal tubing; a fraction collector having a plurality of wells; and an actuator operably coupled to the detector and the fraction collector, wherein the actuator is configured to direct flow of the eluate from the multichannel flow pathway of the detector towards the fraction collector based on a level of the one or more molecules of interest detected by the detector, wherein respective ends of the proximal tubing press up against the first porous frit of each of the plurality of fractionation columns and respective ends of the distal tubing press up against the second porous frit of each of the plurality of fractionation columns when a fluidic circuit through the fractionation system is established. a fractionation system comprising: a pumping system; a detector having at least one sensor arranged relative to a multichannel flow pathway; a monolithic body defining a plurality of fractionation columns, each fractionation column comprising: an inlet chamber at a proximal end of the fractionation column; an outlet chamber at a distal, opposite end of the fractionation column; and a solid phase chamber extending between the inlet chamber and the outlet chamber packed with a metered amount of solid phase between a first porous frit and a second porous frit, wherein the inlet chambers of each of the plurality of fractionation columns are configured to be in fluid communication with the pumping system via proximal tubing, and wherein the outlet chambers of each of the plurality of fractionation columns are configured to direct eluate from the solid phase chamber toward the multichannel flow pathway of the detector via distal tubing; a fraction collector having a plurality of wells; and an actuator operably coupled to the detector and the fraction collector, a pumping system; a detector having at least one sensor arranged relative to a multichannel flow pathway; a monolithic body defining a plurality of fractionation columns, each fractionation column comprising: an inlet chamber at a proximal end of the fractionation column; an outlet chamber at a distal, opposite end of the fractionation column; and a solid phase chamber extending between the inlet chamber and the outlet chamber packed with a metered amount of solid phase between a first porous frit and a second porous frit, an inlet chamber at a proximal end of the fractionation column; an outlet chamber at a distal, opposite end of the fractionation column; and a solid phase chamber extending between the inlet chamber and the outlet chamber packed with a metered amount of solid phase between a first porous frit and a second porous frit, wherein the inlet chambers of each of the plurality of fractionation columns are configured to be in fluid communication with the pumping system via proximal tubing, and wherein the outlet chambers of each of the plurality of fractionation columns are configured to direct eluate from the solid phase chamber toward the multichannel flow pathway of the detector via distal tubing; a fraction collector having a plurality of wells; and an actuator operably coupled to the detector and the fraction collector, wherein the actuator is configured to direct flow of the eluate from the multichannel flow pathway of the detector towards the fraction collector based on a level of the one or more molecules of interest detected by the detector, wherein respective ends of the proximal tubing press up against the first porous frit of each of the plurality of fractionation columns and respective ends of the distal tubing press up against the second porous frit of each of the plurality of fractionation columns when a fluidic circuit through the fractionation system is established.