A lateral flow test device for quantitatively detecting 17-beta estradiol and progesterone in a saliva sample, e.g., a saliva sample from a subject, which device comprises: a first porous matrix that comprises two first test locations on said first porous matrix, one of said first test locations comprising a test reagent being able to compete with 17-beta estradiol in said sample for binding to a labeled Fab fragment that specifically binds to 17-beta estradiol, and the other said first test location comprising a test reagent being able to compete with progesterone in said sample for binding to a labeled Fab fragment that specifically binds to progesterone, wherein the saliva sample can flow laterally along said test device and can pass said two first test locations to form two first detectable signals, wherein: a) said device further comprises a second porous matrix that comprises a second test location on said second porous matrix, said second test location comprising a second test reagent that is a normalization substance that is able to compete with a normalization substance in said saliva sample for binding to a binding reagent for said normalization substance, wherein the normalization substance is transferrin, albumin, creatinine, or a combination thereof, wherein a labeled Fab fragment that specifically binds to the normalization substance is dried on the test device, and wherein the saliva sample can flow laterally along said test device and can pass said second test location to form a second detectable signal, and said first two detectable signals and said second detectable signal are able to be compared to assess levels of 17-beta estradiol and progesterone in said saliva sample; and b) said levels of 17-beta estradiol and progesterone in the saliva sample can be determined and can be combined into a single test result; c) said first porous matrix and said second porous matrix are the same porous matrix; and d) wherein the labeled Fab fragment that specifically binds to 17-beta estradiol, the labeled Fab fragment that specifically binds to progesterone, and the labeled fragment that specifically binds to the normalization substance, are each dried on the test device upstream from the first and second test locations. a) said device further comprises a second porous matrix that comprises a second test location on said second porous matrix, said second test location comprising a second test reagent that is a normalization substance that is able to compete with a normalization substance in said saliva sample for binding to a binding reagent for said normalization substance, wherein the normalization substance is transferrin, albumin, creatinine, or a combination thereof, wherein a labeled Fab fragment that specifically binds to the normalization substance is dried on the test device, and wherein the saliva sample can flow laterally along said test device and can pass said second test location to form a second detectable signal, and said first two detectable signals and said second detectable signal are able to be compared to assess levels of 17-beta estradiol and progesterone in said saliva sample; and b) said levels of 17-beta estradiol and progesterone in the saliva sample can be determined and can be combined into a single test result; c) said first porous matrix and said second porous matrix are the same porous matrix; and d) wherein the labeled Fab fragment that specifically binds to 17-beta estradiol, the labeled Fab fragment that specifically binds to progesterone, and the labeled fragment that specifically binds to the normalization substance, are each dried on the test device upstream from the first and second test locations.