A process for vitrifying biological material comprising the steps of formulating a liquid biological preparation, subjecting the preparation to controlled changes in pressure and temperature to reduce moisture content of the formulation to less than about 5% by weight, thereby vitrifying the biological material; wherein the process further comprises the steps of: (a) adding to the liquid preparation active biologic ingredients, stabilizers, which reduce or eliminate damage induced by subjecting biologic ingredients to cryogenic preservation means, including prilling, vitrification, and freeze-drying, and optionally adding one or more adjuvants; (b) filling vials with the biologic preparation of step (a); (c) loading of vials into temperature-controlled container, wherein the temperature is between -15°C and 10°C, particularly between -10°C and 5°C, and even more particularly about 5°C; (d) reducing the air pressure of the temperature-controlled container until a pressure within the range of 15-30 mbars is obtained; (e) maintaining the pressure obtained during step (d) for between 5 and 20 minutes, particularly between 10 and 15 minutes, to allow the temperature of the product to stabilize and to allow volatile gases, including carbonates, to be released from the biologic preparation, wherein the container temperature remains at about 4°C to about 6°C, or about 5°C during this step; (f) decreasing the container air pressure to about 4 to about 7 mbars, or about 5 mbars, for between about 5 to about 20 minutes; (g) maintaining the pressure of step (f) for about 30 to about 60 minutes, which allows the biologic preparation to become more concentrated; (h) increasing the temperature of the container from negative to positive temperature (between about 30°C to about 50°C) over the course of between 45 and 85 minutes, or about 60 minutes, and holding the pressure constant until reaching about 10°C to about 20°C, or about 15°C; (i) reducing container air pressure to about 1.5 to about 4 mbars, or about 3 mbars, to accelerate the concentration, and until the container temperature reaches and maintains about 30°C for about 30 minutes to about 90 minutes, or 60 minutes; (j) further reducing pressure to between about 0.5 and about 4.0 mbars, or about 1 mbar, and maintaining constant pressure until foaming has completed; (k) further reducing pressure to between about 5 μbar and about 100 μbar, or about 25 μbar, while maintaining the temperature at about 30°C for about 400 to about 2400 or more minutes, until the desired moisture of between about 0.5% to about 15%, or about 1% to about 4%, is obtained; (l) stoppering the vials while in the freeze-dryer container, thereby completing the vitrification method. (a) adding to the liquid preparation active biologic ingredients, stabilizers, which reduce or eliminate damage induced by subjecting biologic ingredients to cryogenic preservation means, including prilling, vitrification, and freeze-drying, and optionally adding one or more adjuvants; (b) filling vials with the biologic preparation of step (a); (c) loading of vials into temperature-controlled container, wherein the temperature is between -15°C and 10°C, particularly between -10°C and 5°C, and even more particularly about 5°C; (d) reducing the air pressure of the temperature-controlled container until a pressure within the range of 15-30 mbars is obtained; (e) maintaining the pressure obtained during step (d) for between 5 and 20 minutes, particularly between 10 and 15 minutes, to allow the temperature of the product to stabilize and to allow volatile gases, including carbonates, to be released from the biologic preparation, wherein the container temperature remains at about 4°C to about 6°C, or about 5°C during this step; (f) decreasing the container air pressure to about 4 to about 7 mbars, or about 5 mbars, for between about 5 to about 20 minutes; (g) maintaining the pressure of step (f) for about 30 to about 60 minutes, which allows the biologic preparation to become more concentrated; (h) increasing the temperature of the container from negative to positive temperature (between about 30°C to about 50°C) over the course of between 45 and 85 minutes, or about 60 minutes, and holding the pressure constant until reaching about 10°C to about 20°C, or about 15°C; (i) reducing container air pressure to about 1.5 to about 4 mbars, or about 3 mbars, to accelerate the concentration, and until the container temperature reaches and maintains about 30°C for about 30 minutes to about 90 minutes, or 60 minutes; (j) further reducing pressure to between about 0.5 and about 4.0 mbars, or about 1 mbar, and maintaining constant pressure until foaming has completed; (k) further reducing pressure to between about 5 μbar and about 100 μbar, or about 25 μbar, while maintaining the temperature at about 30°C for about 400 to about 2400 or more minutes, until the desired moisture of between about 0.5% to about 15%, or about 1% to about 4%, is obtained; (l) stoppering the vials while in the freeze-dryer container, thereby completing the vitrification method.