A process for the preparation of an optically active chiral amine, comprising reacting at least one amino acceptor and at least one amino donor with a -selective ω-transaminase prepared by a method, comprising the following steps: a) providing at least one query biomolecule sequence of at least one -selective ω-transaminase and at least one biomolecule bank, b) searching the biomolecule bank with the query biomolecule sequence to identify a group of first target biomolecule sequences, wherein the first target biomolecule sequences have a degree of sequence identity of at least 20% to the query biomolecule sequence, calculated on amino acid level, c) selecting in the group of first target biomolecule sequences a group of second biomolecule target sequences, which do not comprise, on amino acid level, at least one of the following amino acid sequence motives c1) to c3) with c1) at position 95 to 97 an amino acid sequence Tyr Xa1 Xa2, with Xa1 being an amino acid Ile, Val, Leu, Met, Phe, and Xa2 being an amino acid Arg or Lys or c2) at position 97 to 99 an amino acid sequence Tyr Xaa Gln, with Xaa being an amino acid and in the region from position 105 to 111 an amino acid sequence Arg Xaa Xa3, Xa3 being an amino acid, preferably being His or c3) at position 38 Thr, at position 97 Lys and at position 107 to 109 an amino acid sequence Arg Xa4 Xa5, Xa4 being an amino acid, preferably being Gly and Xa5 being an amino acid, preferably being Tyr and which comprise c4) at position 95 another amino acid than Tyr, Arg, Lys, or at position 95 Tyr, but at position 97 no Arg or Lys and c5) at position 40 no Lys or Arg and c6) in the region from position 161 to 165 at least Lys to identify a group of second target biomolecule sequences and d) providing a biomolecule having the second target biomolecule sequence identified in step c) and being, or coding at least partially, a protein with the activity of a -selective ω-transaminase, and obtaining the optically active chiral amine. a) providing at least one query biomolecule sequence of at least one -selective ω-transaminase and at least one biomolecule bank, b) searching the biomolecule bank with the query biomolecule sequence to identify a group of first target biomolecule sequences, wherein the first target biomolecule sequences have a degree of sequence identity of at least 20% to the query biomolecule sequence, calculated on amino acid level, c) selecting in the group of first target biomolecule sequences a group of second biomolecule target sequences, which do not comprise, on amino acid level, at least one of the following amino acid sequence motives c1) to c3) with c1) at position 95 to 97 an amino acid sequence Tyr Xa1 Xa2, with Xa1 being an amino acid Ile, Val, Leu, Met, Phe, and Xa2 being an amino acid Arg or Lys or c2) at position 97 to 99 an amino acid sequence Tyr Xaa Gln, with Xaa being an amino acid and in the region from position 105 to 111 an amino acid sequence Arg Xaa Xa3, Xa3 being an amino acid, preferably being His or c3) at position 38 Thr, at position 97 Lys and at position 107 to 109 an amino acid sequence Arg Xa4 Xa5, Xa4 being an amino acid, preferably being Gly and Xa5 being an amino acid, preferably being Tyr and which comprise c4) at position 95 another amino acid than Tyr, Arg, Lys, or at position 95 Tyr, but at position 97 no Arg or Lys and c5) at position 40 no Lys or Arg and c6) in the region from position 161 to 165 at least Lys to identify a group of second target biomolecule sequences and c1) at position 95 to 97 an amino acid sequence Tyr Xa1 Xa2, with Xa1 being an amino acid Ile, Val, Leu, Met, Phe, and Xa2 being an amino acid Arg or Lys or c2) at position 97 to 99 an amino acid sequence Tyr Xaa Gln, with Xaa being an amino acid and in the region from position 105 to 111 an amino acid sequence Arg Xaa Xa3, Xa3 being an amino acid, preferably being His or c3) at position 38 Thr, at position 97 Lys and at position 107 to 109 an amino acid sequence Arg Xa4 Xa5, Xa4 being an amino acid, preferably being Gly and Xa5 being an amino acid, preferably being Tyr c4) at position 95 another amino acid than Tyr, Arg, Lys, or at position 95 Tyr, but at position 97 no Arg or Lys and c5) at position 40 no Lys or Arg and c6) in the region from position 161 to 165 at least Lys to identify a group of second target biomolecule sequences and d) providing a biomolecule having the second target biomolecule sequence identified in step c) and being, or coding at least partially, a protein with the activity of a -selective ω-transaminase,