Assembly for ultrasound-assisted manipulation at female reproductive organs of living large mammals, wherein the assembly is intended and designed to be inserted into the rectum of the large mammal for rectal oocyte recovery or rectal embryo transfer or rectal intrauterine insemination, wherein the assembly comprises: - a support pipe with an end element and a handle piece, - a guide pipe (3; 24) provided in the support pipe, - a puncture needle, - an ultrasound head support provided at the distal end of the support pipe and having accommodators for an ultrasound head and the distal end of the guide pipe (3; 24), - wherein the axis of the ultrasound head and of the distal end of the guide pipe (3; 24) are in each case angled with respect to the horizontal axis of the support pipe such that the position of the tip of the puncture needle is in the ultrasound scan range of the ultrasound head, and - if the assembly is intended and designed to be inserted into the rectum of the large mammal for rectal oocyte recovery: - the assembly furthermore comprises a pulse generator which is mounted in a movable manner in the end element in the horizontal direction, - the guide pipe is positioned in the support pipe by means of a fitted positioning element, - the puncture needle has a twin-lumen construction for a flushing process and aspiration process, is arranged in the guide pipe in a movable manner, is connected via its proximal end to the pulse generator in a detachable manner and is fixed in said pulse generator, and - there are connecting pieces at the proximal end of the twin-lumen puncture needle for the outer and inner needles, which connecting pieces are connectable to a medical flushing device and to a vacuum pump and a receptacle by means of pieces of tubing, or - if the assembly is intended and designed to be inserted into the rectum of the large mammal for rectal embryo transfer or rectal intrauterine insemination: - the guide pipe has approximately the length of the support pipe and is mounted at its proximal end in the end element, - there is provided within the guide pipe an insertion aid, in which a single-lumen puncture needle is arranged in a horizontally movable manner and - there is provided in the single-lumen puncture needle a movable catheter, which is provided at its proximal end with a Luer-Lock connector. - a support pipe with an end element and a handle piece, - a guide pipe (3; 24) provided in the support pipe, - a puncture needle, - an ultrasound head support provided at the distal end of the support pipe and having accommodators for an ultrasound head and the distal end of the guide pipe (3; 24), - wherein the axis of the ultrasound head and of the distal end of the guide pipe (3; 24) are in each case angled with respect to the horizontal axis of the support pipe such that the position of the tip of the puncture needle is in the ultrasound scan range of the ultrasound head, and - if the assembly is intended and designed to be inserted into the rectum of the large mammal for rectal oocyte recovery: - the assembly furthermore comprises a pulse generator which is mounted in a movable manner in the end element in the horizontal direction, - the guide pipe is positioned in the support pipe by means of a fitted positioning element, - the puncture needle has a twin-lumen construction for a flushing process and aspiration process, is arranged in the guide pipe in a movable manner, is connected via its proximal end to the pulse generator in a detachable manner and is fixed in said pulse generator, and - there are connecting pieces at the proximal end of the twin-lumen puncture needle for the outer and inner needles, which connecting pieces are connectable to a medical flushing device and to a vacuum pump and a receptacle by means of pieces of tubing, or - if the assembly is intended and designed to be inserted into the rectum of the large mammal for rectal embryo transfer or rectal intrauterine insemination: - the guide pipe has approximately the length of the support pipe and is mounted at its proximal end in the end element, - there is provided within the guide pipe an insertion aid, in which a single-lumen puncture needle is arranged in a horizontally movable manner and - there is provided in the single-lumen puncture needle a movable catheter, which is provided at its proximal end with a Luer-Lock connector.