A disinfection device for high level disinfection of a medical device, comprising: a disinfection chamber (110; 200; 206; 300; 400) having an interior volume; at least one radiation source (202; 302; 402)configured to emit ultraviolet-C (UV-C) radiation into the disinfection chamber (110; 200; 206; 300; 400); at least one radiation sensor (306; 406) configured to detect an amount of UV-C radiation within the interior volume of the disinfection chamber (110; 200; 206; 300; 400); at least one temperature sensor configured to produce a temperature value representative of a temperature of at least one surface of a medical device positioned within the interior volume of the disinfection chamber (110; 200; 206; 300; 400); and a processing unit configured to direct operations of the disinfection device during a calibration procedure and during normal use so as to accomplish high level disinfection of the medical device, while maintaining conditions such that the surface temperature of the medical device does not reach a first temperature threshold, the first temperature threshold being chosen so as to avoid damaging the medical device, the operations including: generating at least one calibration factor during the calibration procedure; applying the at least one calibration factor to adjust at least one of the UV-C radiation values produced by the at least one radiation sensor (306; 406) or an accumulated UV-C radiation value; generating the accumulated UV-C radiation value via an integration process, the accumulated UV-C radiation value representing a total amount of UV-C radiation detected by the at least one radiation sensor (306; 406) during normal use; verifying that the accumulated UV-C radiation value reaches a first radiation threshold so as to comply with high level disinfection; and verifying that the temperature value from the temperature sensor does not reach the first temperature threshold so as to avoid damaging the medical device. a disinfection chamber (110; 200; 206; 300; 400) having an interior volume; at least one radiation source (202; 302; 402)configured to emit ultraviolet-C (UV-C) radiation into the disinfection chamber (110; 200; 206; 300; 400); at least one radiation sensor (306; 406) configured to detect an amount of UV-C radiation within the interior volume of the disinfection chamber (110; 200; 206; 300; 400); at least one temperature sensor configured to produce a temperature value representative of a temperature of at least one surface of a medical device positioned within the interior volume of the disinfection chamber (110; 200; 206; 300; 400); and a processing unit configured to direct operations of the disinfection device during a calibration procedure and during normal use so as to accomplish high level disinfection of the medical device, while maintaining conditions such that the surface temperature of the medical device does not reach a first temperature threshold, the first temperature threshold being chosen so as to avoid damaging the medical device, the operations including: generating at least one calibration factor during the calibration procedure; applying the at least one calibration factor to adjust at least one of the UV-C radiation values produced by the at least one radiation sensor (306; 406) or an accumulated UV-C radiation value; generating the accumulated UV-C radiation value via an integration process, the accumulated UV-C radiation value representing a total amount of UV-C radiation detected by the at least one radiation sensor (306; 406) during normal use; verifying that the accumulated UV-C radiation value reaches a first radiation threshold so as to comply with high level disinfection; and verifying that the temperature value from the temperature sensor does not reach the first temperature threshold so as to avoid damaging the medical device. generating at least one calibration factor during the calibration procedure; applying the at least one calibration factor to adjust at least one of the UV-C radiation values produced by the at least one radiation sensor (306; 406) or an accumulated UV-C radiation value; generating the accumulated UV-C radiation value via an integration process, the accumulated UV-C radiation value representing a total amount of UV-C radiation detected by the at least one radiation sensor (306; 406) during normal use; verifying that the accumulated UV-C radiation value reaches a first radiation threshold so as to comply with high level disinfection; and verifying that the temperature value from the temperature sensor does not reach the first temperature threshold so as to avoid damaging the medical device.