A process of serotyping Salmonella in a sample comprising: performing a first stage assay by adding to a first portion of said sample one or more first stage O-grp-1 primer sets operable to produce an O-grp-1 amplification product from O-grp-1 target sequence; adding to a second portion of said sample one or more first stage H-ag primer sets operable to produce an H-ag amplification product from H-ag target sequence; each of said first stage primer sets comprising a forward primer that hybridizes to a region flanking a respective target sequence under conditions suitable for a polymerase chain reaction, and a reverse primer that hybridizes to a different region of the target sequence under conditions suitable for a polymerase chain reaction, wherein each target sequence is at least a portion of a different Salmonella gene; detecting the presence or absence of each of said amplification products by hybridization to a labeled probe specific to each of said O-grp-1 amplification product(s) and a labeled probe specific to each of said H-ag amplification product(s), wherein the labeled probes specific to each of said H-ag amplification product(s) are set forth in SEQ ID NOs: 42-79; optionally performing a second stage assay by adding to a third portion of said sample one or more second stage primer sets operable to produce a second stage amplification product of second stage target sequence; wherein said second stage target sequence is selected from the group consisting of sequences of the genes encoding Sdf, Vi, and FljB; wherein said second stage primer set comprises a second stage forward primer that hybridizes to a region flanking said second stage target sequence under conditions suitable for a polymerase chain reaction, and a second stage reverse primer that hybridizes to a different region of said second stage target sequence under conditions suitable for a polymerase chain reaction; and detecting the presence of or absence of each of said second stage amplification products by hybridization of a probe specific to each of said second stage amplification products. performing a first stage assay by adding to a first portion of said sample one or more first stage O-grp-1 primer sets operable to produce an O-grp-1 amplification product from O-grp-1 target sequence; adding to a second portion of said sample one or more first stage H-ag primer sets operable to produce an H-ag amplification product from H-ag target sequence; each of said first stage primer sets comprising a forward primer that hybridizes to a region flanking a respective target sequence under conditions suitable for a polymerase chain reaction, and a reverse primer that hybridizes to a different region of the target sequence under conditions suitable for a polymerase chain reaction, wherein each target sequence is at least a portion of a different Salmonella gene; detecting the presence or absence of each of said amplification products by hybridization to a labeled probe specific to each of said O-grp-1 amplification product(s) and a labeled probe specific to each of said H-ag amplification product(s), wherein the labeled probes specific to each of said H-ag amplification product(s) are set forth in SEQ ID NOs: 42-79; optionally performing a second stage assay by adding to a third portion of said sample one or more second stage primer sets operable to produce a second stage amplification product of second stage target sequence; wherein said second stage target sequence is selected from the group consisting of sequences of the genes encoding Sdf, Vi, and FljB; wherein said second stage primer set comprises a second stage forward primer that hybridizes to a region flanking said second stage target sequence under conditions suitable for a polymerase chain reaction, and a second stage reverse primer that hybridizes to a different region of said second stage target sequence under conditions suitable for a polymerase chain reaction; and detecting the presence of or absence of each of said second stage amplification products by hybridization of a probe specific to each of said second stage amplification products.