A disposable cartridge configured for manipulating samples in liquid droplets in or on a digital microfluidics device; the digital microfluidics device being configured to accommodate the disposable cartridge and comprising a base unit, a cartridge accommodation site for taking up the disposable cartridge, an electrode array supported by a bottom substrate and located at said cartridge accommodation site, a central control unit for controlling the selection of individual electrodes of said electrode array and for providing these electrodes with individual voltage pulses for manipulating liquid droplets within said disposable cartridge by electrowetting; the disposable cartridge comprising: a) a bottom layer that is impermeable to liquids and that is configured as a working film with a first hydrophobic surface for manipulating samples in liquid droplets thereon; b) a body with a second hydrophobic surface (17") and with: - at least one through hole for transferring processing liquids, reagents or samples into a gap of the disposable cartridge, or - at least one compartment configured to hold therein processing liquids, reagents or samples and comprising a through hole for delivering at least some of its content to a gap of the disposable cartridge; c) a gap located between the first hydrophobic surface of the bottom layer and the second hydrophobic surface (17") of the body; and d) a gasket attached to the disposable cartridge; characterized in that the gasket is attached to the disposable cartridge outside of the gap and along a circumference of the flexible bottom layer, the gasket being configured to be located between the disposable cartridge and the cartridge accommodation site of the digital microfluidics device the disposable cartridge is accommodated, being configured to seal in the cartridge accommodation site an evacuation space, which is defined by the flexible bottom layer and the gasket of the disposable cartridge, and by the electrode array and the bottom substrate of the digital microfluidics device, and being configured to define the gap; and in that the bottom layer of the disposable cartridge is a flexible film that is sealingly attached to the body along the circumference of the flexible bottom layer; configured to be placed on the cartridge accommodation site; and configured to be attracted to and spread over the cartridge accommodation site of the digital microfluidics system by an underpressure in the evacuation space. a) a bottom layer that is impermeable to liquids and that is configured as a working film with a first hydrophobic surface for manipulating samples in liquid droplets thereon; b) a body with a second hydrophobic surface (17") and with: - at least one through hole for transferring processing liquids, reagents or samples into a gap of the disposable cartridge, or - at least one compartment configured to hold therein processing liquids, reagents or samples and comprising a through hole for delivering at least some of its content to a gap of the disposable cartridge; - at least one through hole for transferring processing liquids, reagents or samples into a gap of the disposable cartridge, or - at least one compartment configured to hold therein processing liquids, reagents or samples and comprising a through hole for delivering at least some of its content to a gap of the disposable cartridge; c) a gap located between the first hydrophobic surface of the bottom layer and the second hydrophobic surface (17") of the body; and d) a gasket attached to the disposable cartridge;