A treatment cycle validation method, comprising operations of washing and disinfecting surgical instruments of the dynamic instrument-holder, DIH, type within an automaton, characterised in that the method implements a test dynamic instrument-holder, test DIH, comprising a wall fully made of transparent material having an inner surface and an outer surface, the test DIH being intended to have a configuration and a structure of a traditional DIH, said wall made of transparent material of the test DIH being intended to enable visual observation of the cleanliness of the inner surface and the outer surface of the wall and observation of the content of the test DIH, and by: a) depositing onto the inner surface and the outer surface of the previously soiled wall of the test DIH, a reagent visually modifying transparency properties of said material of said test DIH, b) integrating said test DIH into the automaton, c) executing the entire treatment cycle within the automaton, d) observing, at the end of the treatment cycle, the wall of said test DIH, e) comparing transparency properties of the wall of the test DIH with those before soiling and adding the reagent, f) validating the execution of the treatment cycle when the entire wall of said test DIH has transparency properties of the material identical to those before soiling and adding the reagent, and by, if the treatment cycle is validated for the test DIH, validating the execution of the treatment cycle for other dynamic instrument-holders potentially present in the automaton, and by, prior to step a), coating the inner surface and the outer surface of said test DIH with soil, said soil being of the protein type, and in that said reagent is a colorimetric reagent intended to interact with the soil to yield a coloured substance modifying transparency properties of the material of the wall of the test DIH soiled. characterised in that the method implements a test dynamic instrument-holder, test DIH, comprising a wall fully made of transparent material having an inner surface and an outer surface, the test DIH being intended to have a configuration and a structure of a traditional DIH, said wall made of transparent material of the test DIH being intended to enable visual observation of the cleanliness of the inner surface and the outer surface of the wall and observation of the content of the test DIH, and by: a) depositing onto the inner surface and the outer surface of the previously soiled wall of the test DIH, a reagent visually modifying transparency properties of said material of said test DIH, b) integrating said test DIH into the automaton, c) executing the entire treatment cycle within the automaton, d) observing, at the end of the treatment cycle, the wall of said test DIH, e) comparing transparency properties of the wall of the test DIH with those before soiling and adding the reagent, f) validating the execution of the treatment cycle when the entire wall of said test DIH has transparency properties of the material identical to those before soiling and adding the reagent, a) depositing onto the inner surface and the outer surface of the previously soiled wall of the test DIH, a reagent visually modifying transparency properties of said material of said test DIH, b) integrating said test DIH into the automaton, c) executing the entire treatment cycle within the automaton, d) observing, at the end of the treatment cycle, the wall of said test DIH, e) comparing transparency properties of the wall of the test DIH with those before soiling and adding the reagent, f) validating the execution of the treatment cycle when the entire wall of said test DIH has transparency properties of the material identical to those before soiling and adding the reagent, and by, if the treatment cycle is validated for the test DIH, validating the execution of the treatment cycle for other dynamic instrument-holders potentially present in the automaton, and by, prior to step a), coating the inner surface and the outer surface of said test DIH with soil, said soil being of the protein type, and in that said reagent is a colorimetric reagent intended to interact with the soil to yield a coloured substance modifying transparency properties of the material of the wall of the test DIH soiled.