A glucose sensor insertion assembly, comprising: an applicator assembly comprising a housing, an interior, and a distal end; a sensor electronics assembly releasably retained within the interior of the applicator assembly, the sensor electronics assembly comprising sensor electronics comprising a processor, a communications facility, and a circuit board, wherein the communications facility is configured to communicate data indicative of a glucose level detected by the glucose sensor to a receiver unit, the sensor electronics disposed within a housing of the sensor electronics assembly, wherein the sensor electronics are configured to electrically couple with a proximal portion of a glucose sensor and wherein a distal portion of the glucose sensor is configured to be positioned under a skin surface and in contact with a bodily fluid of a subject; and a removable cap configured to be threadably engaged with the distal end of the applicator assembly, wherein the housing of the applicator assembly comprises integrally formed grip features, wherein an end of the removable cap comprises one or more openings configured for passage of a sterilizing gas between an exterior of the glucose sensor insertion assembly and the interior of the glucose sensor insertion assembly, wherein the one or more openings are covered by a seal, wherein the seal is configured to permit the passage of ethylene oxide between the exterior of the glucose sensor insertion assembly and the interior of the glucose sensor insertion assembly, and wherein the interior of the applicator assembly comprises a sterile environment when the removable cap is threadably engaged with the distal end of the applicator assembly. an applicator assembly comprising a housing, an interior, and a distal end; a sensor electronics assembly releasably retained within the interior of the applicator assembly, the sensor electronics assembly comprising sensor electronics comprising a processor, a communications facility, and a circuit board, wherein the communications facility is configured to communicate data indicative of a glucose level detected by the glucose sensor to a receiver unit, the sensor electronics disposed within a housing of the sensor electronics assembly, wherein the sensor electronics are configured to electrically couple with a proximal portion of a glucose sensor and wherein a distal portion of the glucose sensor is configured to be positioned under a skin surface and in contact with a bodily fluid of a subject; and a removable cap configured to be threadably engaged with the distal end of the applicator assembly, wherein the housing of the applicator assembly comprises integrally formed grip features, wherein an end of the removable cap comprises one or more openings configured for passage of a sterilizing gas between an exterior of the glucose sensor insertion assembly and the interior of the glucose sensor insertion assembly, wherein the one or more openings are covered by a seal, wherein the seal is configured to permit the passage of ethylene oxide between the exterior of the glucose sensor insertion assembly and the interior of the glucose sensor insertion assembly, and wherein the interior of the applicator assembly comprises a sterile environment when the removable cap is threadably engaged with the distal end of the applicator assembly. wherein the housing of the applicator assembly comprises integrally formed grip features, wherein an end of the removable cap comprises one or more openings configured for passage of a sterilizing gas between an exterior of the glucose sensor insertion assembly and the interior of the glucose sensor insertion assembly, wherein the one or more openings are covered by a seal, wherein the seal is configured to permit the passage of ethylene oxide between the exterior of the glucose sensor insertion assembly and the interior of the glucose sensor insertion assembly, and wherein the interior of the applicator assembly comprises a sterile environment when the removable cap is threadably engaged with the distal end of the applicator assembly.