A high capacity, closed, cryopreservation cell banking system, comprising: a. a user interface for controlling and monitoring the dispensing of liquid biological materials into a plurality of cryopreservation bags; b. a plurality of cryopreservation bags, wherein each bag is a high capacity cryopreservation bag, which is adapted for use in a high capacity biological material, closed cryopreservation banking system, and which is resistant to liquid nitrogen temperatures; c. a plurality of fluid connections, adapted to fill the plurality of bags; d. a thermo-sealing means, for sealing tubing and bags; e. a plurality of metal cassettes for containing, freezing, storing and thawing the bags; and wherein said high capacity cryopreservation bag comprises: f. a first fluorinated ethylene propylene enclosure; g. at least one rigid FEP inlet conduit, in fluid connection with the first enclosure, and connectable to a first flexible sterile-weldable conduit which is PVC tubing; h. at least one rigid FEP outlet conduit, in fluid connection with the first enclosure, and connectable to a second flexible sterile-weldable conduit which is PVC tubing; and i. a FEP pouch, which is initially open at one end, and which is contiguous with the first enclosure, but thermosealed therefrom, and encircling both the rigid inlet conduit and the rigid outlet conduit, such that the pouch is neither in fluid communication with the first enclosure, nor the inlet nor outlet conduits; and wherein the pouch extends sufficiently far enough such that when the first enclosure has been filled with the biological material, the PVC tubes may be withdrawn into the pouch, which can then be thermosealed to form a second enclosure, which now contains both PVC tubes. a. a user interface for controlling and monitoring the dispensing of liquid biological materials into a plurality of cryopreservation bags; b. a plurality of cryopreservation bags, wherein each bag is a high capacity cryopreservation bag, which is adapted for use in a high capacity biological material, closed cryopreservation banking system, and which is resistant to liquid nitrogen temperatures; c. a plurality of fluid connections, adapted to fill the plurality of bags; d. a thermo-sealing means, for sealing tubing and bags; e. a plurality of metal cassettes for containing, freezing, storing and thawing the bags; and f. a first fluorinated ethylene propylene enclosure; g. at least one rigid FEP inlet conduit, in fluid connection with the first enclosure, and connectable to a first flexible sterile-weldable conduit which is PVC tubing; h. at least one rigid FEP outlet conduit, in fluid connection with the first enclosure, and connectable to a second flexible sterile-weldable conduit which is PVC tubing; and i. a FEP pouch, which is initially open at one end, and which is contiguous with the first enclosure, but thermosealed therefrom, and encircling both the rigid inlet conduit and the rigid outlet conduit, such that the pouch is neither in fluid communication with the first enclosure, nor the inlet nor outlet conduits; and wherein the pouch extends sufficiently far enough such that when the first enclosure has been filled with the biological material, the PVC tubes may be withdrawn into the pouch, which can then be thermosealed to form a second enclosure, which now contains both PVC tubes.