A method for detecting labeled nucleotides in a DNA sequence comprising the steps of a) providing a nucleic acid template and primer capable of hybridizing to said template so as to form a primer/template hybridization complex, a cleave reagent comprising di-mercaptopropanesulfonate, DNA polymerase, and 4 differently labeled populations of deoxynucleoside triphosphate compounds representing analogs of A, G, C and T or U, wherein each of said labeled deoxynucleoside triphosphate compounds comprise a nucleobase and a sugar, said sugar comprising a cleavable protecting group on the 3'-O, wherein said cleavable protecting group comprises methylenedisulfide, wherein said labeled deoxynucleoside triphosphate comprise a label attached via a cleavable oxymethylenedisulfide-containing linker to the nucleobase and a cleave scavenger reagent; b) adding said DNA polymerase and said labeled populations of deoxynucleoside triphosphate compounds to said primer and template so as to create a reaction mixture; c) subjecting said reaction mixture to conditions which enable a DNA polymerase catalyzed primer extension reaction so as to create a modified primer/template hybridization complex, wherein a first labeled deoxynucleoside triphosphate is incorporated; d) detecting said label of said first labeled deoxynucleoside triphosphate in said modified primer/template hybridization complex, wherein said detecting allows for the determination of the nucleobase of said incorporated first deoxynucleoside triphosphate; e) introducing said cleave reagent under conditions so as to remove said cleavable protecting group and said detectable label from said modified primer/template hybridization complex; and f) introducing said cleave scavenger reagent. a) providing a nucleic acid template and primer capable of hybridizing to said template so as to form a primer/template hybridization complex, a cleave reagent comprising di-mercaptopropanesulfonate, DNA polymerase, and 4 differently labeled populations of deoxynucleoside triphosphate compounds representing analogs of A, G, C and T or U, wherein each of said labeled deoxynucleoside triphosphate compounds comprise a nucleobase and a sugar, said sugar comprising a cleavable protecting group on the 3'-O, wherein said cleavable protecting group comprises methylenedisulfide, wherein said labeled deoxynucleoside triphosphate comprise a label attached via a cleavable oxymethylenedisulfide-containing linker to the nucleobase and a cleave scavenger reagent; b) adding said DNA polymerase and said labeled populations of deoxynucleoside triphosphate compounds to said primer and template so as to create a reaction mixture; c) subjecting said reaction mixture to conditions which enable a DNA polymerase catalyzed primer extension reaction so as to create a modified primer/template hybridization complex, wherein a first labeled deoxynucleoside triphosphate is incorporated; d) detecting said label of said first labeled deoxynucleoside triphosphate in said modified primer/template hybridization complex, wherein said detecting allows for the determination of the nucleobase of said incorporated first deoxynucleoside triphosphate; e) introducing said cleave reagent under conditions so as to remove said cleavable protecting group and said detectable label from said modified primer/template hybridization complex; and f) introducing said cleave scavenger reagent.