A method for producing a target substance comprising: culturing a coryneform bacterium having an ability to produce a target substance in a medium containing xylose to produce and accumulate the target substance in the medium; and collecting the target substance from the medium, wherein xylose assimilability of the bacterium has been improved by attenuation of expression of the NCgl2954 gene on the chromosome of the bacterium as compared with Corynebacterium glutamicum strain ATCC 13869, disruption of the NCgl2954 gene, or introduction of a mutation into a coding region and/or an expression control region of the NCgl2954 gene, wherein the mutation consists of one or more of mutations selected from the mutations of to mentioned below: a mutation for replacing an amino acid residue corresponding to the tyrosine residue at the position 377 of SEQ ID NO: 14 with an amino acid residue other than tyrosine residue; a mutation for replacing an amino acid residue corresponding to the leucine residue at the position 365 of SEQ ID NO: 14 with an amino acid residue other than leucine residue; a mutation for replacing an amino acid residue corresponding to the leucine residue at the position 366 of SEQ ID NO: 14 with an amino acid residue other than leucine residue; a mutation for replacing an amino acid residue corresponding to the alanine residue at the position 367 of SEQ ID NO: 14 with an amino acid residue other than alanine residue; a mutation for deleting amino acid residues corresponding to the amino acid residue at the position 368 of SEQ ID NO: 14 and the following amino acid residues, or wherein the method comprises a step of improving the xylose assimilability of the bacterium by attenuation of expression of the NCgl2954 gene on the chromosome of the bacterium or disruption of the NCgl2954 gene. culturing a coryneform bacterium having an ability to produce a target substance in a medium containing xylose to produce and accumulate the target substance in the medium; and collecting the target substance from the medium, wherein xylose assimilability of the bacterium has been improved by attenuation of expression of the NCgl2954 gene on the chromosome of the bacterium as compared with Corynebacterium glutamicum strain ATCC 13869, disruption of the NCgl2954 gene, or introduction of a mutation into a coding region and/or an expression control region of the NCgl2954 gene, wherein the mutation consists of one or more of mutations selected from the mutations of to mentioned below: a mutation for replacing an amino acid residue corresponding to the tyrosine residue at the position 377 of SEQ ID NO: 14 with an amino acid residue other than tyrosine residue; a mutation for replacing an amino acid residue corresponding to the leucine residue at the position 365 of SEQ ID NO: 14 with an amino acid residue other than leucine residue; a mutation for replacing an amino acid residue corresponding to the leucine residue at the position 366 of SEQ ID NO: 14 with an amino acid residue other than leucine residue; a mutation for replacing an amino acid residue corresponding to the alanine residue at the position 367 of SEQ ID NO: 14 with an amino acid residue other than alanine residue; a mutation for deleting amino acid residues corresponding to the amino acid residue at the position 368 of SEQ ID NO: 14 and the following amino acid residues, or a mutation for replacing an amino acid residue corresponding to the tyrosine residue at the position 377 of SEQ ID NO: 14 with an amino acid residue other than tyrosine residue; a mutation for replacing an amino acid residue corresponding to the leucine residue at the position 365 of SEQ ID NO: 14 with an amino acid residue other than leucine residue; a mutation for replacing an amino acid residue corresponding to the leucine residue at the position 366 of SEQ ID NO: 14 with an amino acid residue other than leucine residue; a mutation for replacing an amino acid residue corresponding to the alanine residue at the position 367 of SEQ ID NO: 14 with an amino acid residue other than alanine residue; a mutation for deleting amino acid residues corresponding to the amino acid residue at the position 368 of SEQ ID NO: 14 and the following amino acid residues, or wherein the method comprises a step of improving the xylose assimilability of the bacterium by attenuation of expression of the NCgl2954 gene on the chromosome of the bacterium or disruption of the NCgl2954 gene.