A method, comprising the following steps: (a) penetrating an elastic septum (32, 132) of a device (10, 110, 301, 401, 501) with an injection or filling member or needle, wherein the device (10, 110, 301, 401, 501) defines a sterile interior sealed from ambient atmosphere and in fluid communication with the elastic septum (32, 132); (b) during the penetrating step, forming an annular interface between the elastic septum (32, 132) and the injection or filling member or needle extending axially between a penetration point on an interior surface of the elastic septum (32, 132) in fluid communication with the sterile interior, and an exterior surface of the septum (32, 132) engaging the injection or filling member or needle, wherein the annular interface is defined by a portion of the penetrated elastic septum (32, 132) extending annularly about the injection or filling member or needle substantially throughout an axial distance of at least about 1/2 mm extending between the interior and exterior points of contact (100, 102) between the penetrated septum (32, 132) and injection or filling member or needle, and de-contaminating the injection or filling member or needle by (i) imparting sufficient friction force between the elastic septum (32, 132) and injection or filling member or needle at the annular interface, and/or (ii) sufficient elongation of the elastic septum (32, 132) at the annular interface during penetration; (c) flowing a substance through the injection or filling member or needle and between the injection or filling member or needle and the sterile interior of the device; (d) withdrawing the injection or filling member or needle from the elastic septum (32, 132); (e) allowing the elastic septum (32, 132) to reseal itself at a penetration aperture resulting from withdrawal of the injection or filling member or needle; and (f) maintaining the interior sterile throughout steps (a) through (e). (a) penetrating an elastic septum (32, 132) of a device (10, 110, 301, 401, 501) with an injection or filling member or needle, wherein the device (10, 110, 301, 401, 501) defines a sterile interior sealed from ambient atmosphere and in fluid communication with the elastic septum (32, 132); (b) during the penetrating step, forming an annular interface between the elastic septum (32, 132) and the injection or filling member or needle extending axially between a penetration point on an interior surface of the elastic septum (32, 132) in fluid communication with the sterile interior, and an exterior surface of the septum (32, 132) engaging the injection or filling member or needle, wherein the annular interface is defined by a portion of the penetrated elastic septum (32, 132) extending annularly about the injection or filling member or needle substantially throughout an axial distance of at least about 1/2 mm extending between the interior and exterior points of contact (100, 102) between the penetrated septum (32, 132) and injection or filling member or needle, and de-contaminating the injection or filling member or needle by (i) imparting sufficient friction force between the elastic septum (32, 132) and injection or filling member or needle at the annular interface, and/or (ii) sufficient elongation of the elastic septum (32, 132) at the annular interface during penetration; (c) flowing a substance through the injection or filling member or needle and between the injection or filling member or needle and the sterile interior of the device; (d) withdrawing the injection or filling member or needle from the elastic septum (32, 132); (e) allowing the elastic septum (32, 132) to reseal itself at a penetration aperture resulting from withdrawal of the injection or filling member or needle; and (f) maintaining the interior sterile throughout steps (a) through (e).