A portable sterilization device (100, 200, 300) for use with one or more sterilants, the portable sterilization device comprising: a sealable sterilization chamber (110, 210, 310, 510, 900) having one or more walls defining a sealable internal volume having a height, a width, and a depth; a sterilant delivery device (180, 280) comprising an aerosolizing component and a sterilant outlet, the sterilant outlet being disposed within or at a boundary of the internal volume and oriented to disperse aerosolized sterilant into the internal volume; wherein the sterilant delivery device comprises a plurality of sterilant outlets disposed within or at the boundary of the internal volume of the sterilization chamber; an ozone delivery device (160, 560) comprising an ozone outlet disposed within or at the boundary of the internal volume such that ozone is delivered to the internal volume; wherein the ozone delivery device comprises an ozone source in fluid communication with a plurality of ozone outlets through a manifold; an ozone generation assembly; characterized in a control panel configured to activate the sterilant delivery device and the ozone delivery device when the sterilization chamber is sealed, whereby an oxidative aerosol sterilant is created within the internal volume by mixing of the aerosolized sterilant and the ozone in the internal volume of the sealable sterilization chamber, and wherein the sterilant is substantially prevented from mixing with ozone prior to being dispersed through the sterilant outlet into the internal volume by the sterilant delivery device, and wherein at least a portion of the plurality of sterilant outlets and at least a portion of the plurality of ozone outlets are incorporated into or form a pin mat disposed within the internal volume of the sterilization chamber (110, 210, 310, 510, 900), the pin mat being adapted to support an object to be sterilized in the portable sterilization device (100, 200, 300). a sealable sterilization chamber (110, 210, 310, 510, 900) having one or more walls defining a sealable internal volume having a height, a width, and a depth; a sterilant delivery device (180, 280) comprising an aerosolizing component and a sterilant outlet, the sterilant outlet being disposed within or at a boundary of the internal volume and oriented to disperse aerosolized sterilant into the internal volume; wherein the sterilant delivery device comprises a plurality of sterilant outlets disposed within or at the boundary of the internal volume of the sterilization chamber; an ozone delivery device (160, 560) comprising an ozone outlet disposed within or at the boundary of the internal volume such that ozone is delivered to the internal volume; wherein the ozone delivery device comprises an ozone source in fluid communication with a plurality of ozone outlets through a manifold; an ozone generation assembly; characterized in a control panel configured to activate the sterilant delivery device and the ozone delivery device when the sterilization chamber is sealed, whereby an oxidative aerosol sterilant is created within the internal volume by mixing of the aerosolized sterilant and the ozone in the internal volume of the sealable sterilization chamber, and wherein the sterilant is substantially prevented from mixing with ozone prior to being dispersed through the sterilant outlet into the internal volume by the sterilant delivery device, and wherein at least a portion of the plurality of sterilant outlets and at least a portion of the plurality of ozone outlets are incorporated into or form a pin mat disposed within the internal volume of the sterilization chamber (110, 210, 310, 510, 900), the pin mat being adapted to support an object to be sterilized in the portable sterilization device (100, 200, 300).