A polymerized indicator-binding compound complex composition comprising a first polymer directly or indirectly linked to a second polymer, where the first polymer comprises at least a first indicator molecule directly or indirectly linked to a second indicator molecule and at least one binding compound directly or indirectly linked to at least one indicator molecule, and where the second polymer comprises at least a third indicator molecule directly or indirectly linked to a fourth indicator molecule, and where directly linked molecules comprise covalent bonds between the molecules, and indirectly linked molecules comprise covalent bonds between the molecules and a linker moiety, where the linker moiety is either homobifunctional or heterobifunctional and wherein the heterobifunctional linker moiety comprises a hydrophilic spacer comprising (poly)ethylene oxide or (poly)ethylene glycol reactive with an amine or sulfhydryl, where the first through fourth indicator molecules are the same, and where the composition is a polymerized indicator-binding compound complex lacking a carrier backbone, where the directly linked molecules comprise covalent bonds formed between an amine group on one molecule and an aldehyde group from an oxidized carbohydrate group on another molecule, where the indicator molecule and binding compound are proteins. a first polymer directly or indirectly linked to a second polymer, where the first polymer comprises at least a first indicator molecule directly or indirectly linked to a second indicator molecule and at least one binding compound directly or indirectly linked to at least one indicator molecule, and where the second polymer comprises at least a third indicator molecule directly or indirectly linked to a fourth indicator molecule, and where directly linked molecules comprise covalent bonds between the molecules, and indirectly linked molecules comprise covalent bonds between the molecules and a linker moiety, where the linker moiety is either homobifunctional or heterobifunctional and wherein the heterobifunctional linker moiety comprises a hydrophilic spacer comprising (poly)ethylene oxide or (poly)ethylene glycol reactive with an amine or sulfhydryl, where the first through fourth indicator molecules are the same, and where the composition is a polymerized indicator-binding compound complex lacking a carrier backbone, where the first polymer comprises at least a first indicator molecule directly or indirectly linked to a second indicator molecule and at least one binding compound directly or indirectly linked to at least one indicator molecule, and where the second polymer comprises at least a third indicator molecule directly or indirectly linked to a fourth indicator molecule, and where directly linked molecules comprise covalent bonds between the molecules, and indirectly linked molecules comprise covalent bonds between the molecules and a linker moiety, where the linker moiety is either homobifunctional or heterobifunctional and wherein the heterobifunctional linker moiety comprises a hydrophilic spacer comprising (poly)ethylene oxide or (poly)ethylene glycol reactive with an amine or sulfhydryl, where the first through fourth indicator molecules are the same, and where the composition is a polymerized indicator-binding compound complex lacking a carrier backbone,