A surface plasmon resonance fluorescence analysis device for exciting a fluorescence material for labelling a substance to be detected to which an analysis chip including a prism having a metal film suitable for receiving a substance to be detected provided on one surface of the prism is attachable, and the surface plasmon resonance fluorescence analysis device comprising: a chip holder configured to detachably hold the analysis chip; a light source configured to emit excitation light and to irradiate the metal film through the prism; an angle adjusting section configured to adjust an incident angle of the excitation light with respect to the metal film to irradiate the metal film with the excitation light through the prism at a predetermined incident angle; a first light sensor configured to detect fluorescence emitted from the fluorescence material; a light reception optical system configured to guide light emitted from the metal film to the first light sensor, wherein the light reception optical system includes a first lens; an excitation light filter, wherein the excitation light filter is configured to be an excitation light reflection filter disposed in the light reception optical system and configured to reflect light having a wavelength same as that of the excitation light emitted from the light source in the light emitted from the metal film; a second light sensor and a control section configured to control the angle adjusting section; wherein the control section is configured to control the incident angle of the excitation light with respect to the metal film to be adjusted by the angle adjusting section on a basis of a detection result of the light obtained by the second light sensor, characterized in that - the second light sensor is configured to detect the light reflected by the excitation light reflection filter and - the light reception optical system includes a second lens, wherein the first lens and the second lens are disposed to bring fluorescence from the metal film to the first light sensor; - the first lens, the excitation light reflection filter and the second lens are disposed in this order from a side of the metal film; and - the second light sensor is configured to detect light reflected by the excitation light reflection filter and condensed by the first lens, - wherein the second light sensor is disposed at a position where light reflected by the excitation light reflection filter and subsequently condensed by the first lens can be detected, wherein said reflected light reaches the second light sensor without returning to analysis chip. a chip holder configured to detachably hold the analysis chip; a light source configured to emit excitation light and to irradiate the metal film through the prism; an angle adjusting section configured to adjust an incident angle of the excitation light with respect to the metal film to irradiate the metal film with the excitation light through the prism at a predetermined incident angle; a first light sensor configured to detect fluorescence emitted from the fluorescence material; a light reception optical system configured to guide light emitted from the metal film to the first light sensor, wherein the light reception optical system includes a first lens; an excitation light filter, wherein the excitation light filter is configured to be an excitation light reflection filter disposed in the light reception optical system and configured to reflect light having a wavelength same as that of the excitation light emitted from the light source in the light emitted from the metal film; a second light sensor and a control section configured to control the angle adjusting section; wherein the control section is configured to control the incident angle of the excitation light with respect to the metal film to be adjusted by the angle adjusting section on a basis of a detection result of the light obtained by the second light sensor, characterized in that - the second light sensor is configured to detect the light reflected by the excitation light reflection filter and - the light reception optical system includes a second lens, wherein the first lens and the second lens are disposed to bring fluorescence from the metal film to the first light sensor; - the first lens, the excitation light reflection filter and the second lens are disposed in this order from a side of the metal film; and - the second light sensor is configured to detect light reflected by the excitation light reflection filter and condensed by the first lens, - wherein the second light sensor is disposed at a position where light reflected by the excitation light reflection filter and subsequently condensed by the first lens can be detected, wherein said reflected light reaches the second light sensor without returning to analysis chip. - the second light sensor is configured to detect the light reflected by the excitation light reflection filter and - the light reception optical system includes a second lens, wherein the first lens and the second lens are disposed to bring fluorescence from the metal film to the first light sensor; - the first lens, the excitation light reflection filter and the second lens are disposed in this order from a side of the metal film; and - the second light sensor is configured to detect light reflected by the excitation light reflection filter and condensed by the first lens, - wherein the second light sensor is disposed at a position where light reflected by the excitation light reflection filter and subsequently condensed by the first lens can be detected, wherein said reflected light reaches the second light sensor without returning to analysis chip.