A method for determining zygosity of a corn plant comprising an AAD-1 corn event DAS-40278-9, said event comprising a transgene construct comprising an AAD-1 gene, said transgene construct being flanked by a 5' flanking corn genomic DNA and a 3' flanking corn genomic DNA, said method comprising: obtaining a DNA sample of genomic DNA from said corn plant; producing a contacted sample by contacting said DNA sample with a. a first event primer and a second event primer, wherein said first event primer specifically binds said transgene construct as defined by residues 1874-6689 of SEQ ID NO:29, said second event primer specifically binds said 5' corn genomic flanking DNA as defined by residues 1-1873 of SEQ ID NO:29 or said 3' corn genomic flanking DNA as defined by residues 6690-8557 of SEQ ID NO:29, and wherein said first event primer and said second event primer produce an event amplicon when subjected to a PCR reaction b. a reference forward primer and a reference reverse primer that produce a reference amplicon from an endogenous corn reference gene when subjected to a PCR reaction, wherein said reference gene: i. is an endogenous Zea mays invertase gene; or ii. comprises a sequence selected from the group consisting of SEQ ID NO:5, SEQ ID NO:6, and SEQ ID NO:7 or a sequence having at least 90 % sequence identity to any one of the aforementioned sequences, c. a fluorescent event probe that hybridizes with said event amplicon d. a fluorescent reference probe that hybridizes with said reference amplicon; subjecting said contacted sample to a fluorescence-based hydrolysis probe assay which releases the fluorescent moiety of the probe from the quenching moiety of the probe; quantitating said florescent event probe that hybridized to said event amplicon; quantitating said florescent reference probe that hybridized to said reference amplicon; comparing amounts of hybridized florescent event probe to hybridized florescent reference probe; and determining zygosity of DAS-40278-9 by comparing florescence ratios of hybridized fluorescent event probe and hybridized fluorescent reference probe. obtaining a DNA sample of genomic DNA from said corn plant; producing a contacted sample by contacting said DNA sample with a. a first event primer and a second event primer, wherein said first event primer specifically binds said transgene construct as defined by residues 1874-6689 of SEQ ID NO:29, said second event primer specifically binds said 5' corn genomic flanking DNA as defined by residues 1-1873 of SEQ ID NO:29 or said 3' corn genomic flanking DNA as defined by residues 6690-8557 of SEQ ID NO:29, and wherein said first event primer and said second event primer produce an event amplicon when subjected to a PCR reaction b. a reference forward primer and a reference reverse primer that produce a reference amplicon from an endogenous corn reference gene when subjected to a PCR reaction, wherein said reference gene: i. is an endogenous Zea mays invertase gene; or ii. comprises a sequence selected from the group consisting of SEQ ID NO:5, SEQ ID NO:6, and SEQ ID NO:7 or a sequence having at least 90 % sequence identity to any one of the aforementioned sequences, c. a fluorescent event probe that hybridizes with said event amplicon d. a fluorescent reference probe that hybridizes with said reference amplicon; a. a first event primer and a second event primer, wherein said first event primer specifically binds said transgene construct as defined by residues 1874-6689 of SEQ ID NO:29, said second event primer specifically binds said 5' corn genomic flanking DNA as defined by residues 1-1873 of SEQ ID NO:29 or said 3' corn genomic flanking DNA as defined by residues 6690-8557 of SEQ ID NO:29, and wherein said first event primer and said second event primer produce an event amplicon when subjected to a PCR reaction b. a reference forward primer and a reference reverse primer that produce a reference amplicon from an endogenous corn reference gene when subjected to a PCR reaction, wherein said reference gene: i. is an endogenous Zea mays invertase gene; or ii. comprises a sequence selected from the group consisting of SEQ ID NO:5, SEQ ID NO:6, and SEQ ID NO:7 or a sequence having at least 90 % sequence identity to any one of the aforementioned sequences, i. is an endogenous Zea mays invertase gene; or ii. comprises a sequence selected from the group consisting of SEQ ID NO:5, SEQ ID NO:6, and SEQ ID NO:7 or a sequence having at least 90 % sequence identity to any one of the aforementioned sequences, c. a fluorescent event probe that hybridizes with said event amplicon d. a fluorescent reference probe that hybridizes with said reference amplicon;