mhurhangee/ep-ind-claims huggingface dataset:
A method for generating a viral genome comprising a nucleic acid molecule encoding a heterologous immunogenic peptide, wherein the viral genome is the genome of a flavivirus, the method comprising the steps of: (i) providing a target viral gene; (ii) subjecting the target viral gene to mutagenesis to insert a restriction site; (iii) ligating a nucleic acid molecule encoding a heterologous immunogenic peptide into the restriction site of the target viral gene to generate a mutant gene library; (iv) transfecting cells with the viral genomes of step (iii) to initiate virus replication; and (v) selecting viable virus recombinants with an antibody against the immunogenic peptide; wherein the target viral gene is provided (a) in a shuttle vector and, after ligation of the nucleic acid molecule encoding the heterologous peptide into the site of mutagenesis of the target viral gene, the method further comprises the step of introducing the mutated target viral gene into a viral genome from which the target viral gene was derived, in place of the corresponding viral gene lacking the insertion, to generate viral genomes comprising insertions, or (b) in the context of an intact viral genome, and the method generates viral genomes comprising insertions. (i) providing a target viral gene; (ii) subjecting the target viral gene to mutagenesis to insert a restriction site; (iii) ligating a nucleic acid molecule encoding a heterologous immunogenic peptide into the restriction site of the target viral gene to generate a mutant gene library; (iv) transfecting cells with the viral genomes of step (iii) to initiate virus replication; and (v) selecting viable virus recombinants with an antibody against the immunogenic peptide; wherein the target viral gene is provided (a) in a shuttle vector and, after ligation of the nucleic acid molecule encoding the heterologous peptide into the site of mutagenesis of the target viral gene, the method further comprises the step of introducing the mutated target viral gene into a viral genome from which the target viral gene was derived, in place of the corresponding viral gene lacking the insertion, to generate viral genomes comprising insertions, or (b) in the context of an intact viral genome, and the method generates viral genomes comprising insertions.
A kit for use in a method for detecting antibodies in a sample, in particular by indirect immunofluorescence, comprising: an antigen chip, said antigen chip comprising: (i) a planar substrate surface; and (ii) antigen spots (11a,..., 11e) applied to the substrate surface in a predetermined pattern and containing a first fluorescent dye; and a probe, in particular a secondary antibody, which is labelled with a second fluorescent dye, wherein the first and the second fluorescence have an excitation maximum between 500 nm and 540 nm and wherein the emission maxima of the two fluorescent dyes are at least 5 nm apart. an antigen chip, said antigen chip comprising: (i) a planar substrate surface; and (ii) antigen spots (11a,..., 11e) applied to the substrate surface in a predetermined pattern and containing a first fluorescent dye; and (i) a planar substrate surface; and (ii) antigen spots (11a,..., 11e) applied to the substrate surface in a predetermined pattern and containing a first fluorescent dye; and a probe, in particular a secondary antibody, which is labelled with a second fluorescent dye, wherein the first and the second fluorescence have an excitation maximum between 500 nm and 540 nm and wherein the emission maxima of the two fluorescent dyes are at least 5 nm apart.
A secondary battery, comprising: a negative electrode plate that comprises a negative electrode film, wherein the negative electrode film comprises a negative electrode active material; electrolyte that comprises an electrolyte salt, an organic solvent and an additive, wherein the negative electrode active material comprises a silicon-based material; the organic solvent consists of dimethyl carbonate, ethylene carbonate, and ethyl methyl carbonate; the additive comprises one or more of the compounds shown in Formula 1 below; where, R 1 is selected from one or more of C2~C4 alkylene or halogenated alkylene, C2~C4 alkenylene or halogenated alkenylene, and C6~C18 arylene; the mass ratio of the compound shown in Formula 1 in the electrolyte is ≤1%; and the mass ratio of the dimethyl carbonate in the organic solvent is ≤20%; the mass ratio of EC in the organic solvent is ≤15%; preferably, the mass ratio of EC in the organic solvent is ≤10%; and the mass ratio of EMC in the organic solvent is 65%~95%; preferably, the mass ratio of EMC in the organic solvent is 75%~95%. a negative electrode plate that comprises a negative electrode film, wherein the negative electrode film comprises a negative electrode active material; electrolyte that comprises an electrolyte salt, an organic solvent and an additive, wherein the negative electrode active material comprises a silicon-based material; the organic solvent consists of dimethyl carbonate, ethylene carbonate, and ethyl methyl carbonate; the additive comprises one or more of the compounds shown in Formula 1 below; where, R 1 is selected from one or more of C2~C4 alkylene or halogenated alkylene, C2~C4 alkenylene or halogenated alkenylene, and C6~C18 arylene; the mass ratio of the compound shown in Formula 1 in the electrolyte is ≤1%; and the mass ratio of the dimethyl carbonate in the organic solvent is ≤20%; the mass ratio of EC in the organic solvent is ≤15%; preferably, the mass ratio of EC in the organic solvent is ≤10%; and the mass ratio of EMC in the organic solvent is 65%~95%; preferably, the mass ratio of EMC in the organic solvent is 75%~95%. the negative electrode active material comprises a silicon-based material; the organic solvent consists of dimethyl carbonate, ethylene carbonate, and ethyl methyl carbonate; the additive comprises one or more of the compounds shown in Formula 1 below; where, R 1 is selected from one or more of C2~C4 alkylene or halogenated alkylene, C2~C4 alkenylene or halogenated alkenylene, and C6~C18 arylene; the mass ratio of the compound shown in Formula 1 in the electrolyte is ≤1%; and the mass ratio of the dimethyl carbonate in the organic solvent is ≤20%; the mass ratio of EC in the organic solvent is ≤15%; preferably, the mass ratio of EC in the organic solvent is ≤10%; and the mass ratio of EMC in the organic solvent is 65%~95%; preferably, the mass ratio of EMC in the organic solvent is 75%~95%.
A surgical fastening device, comprising: an elongate shaft; and an end effector coupled to a distal end of the elongate shaft, the end effector including a cartridge jaw and an anvil pivotally coupled to one another at a pivot point, and a cam pin disposed within a cam slot formed in each of the cartridge jaw and the anvil, the cam pin being slidable within the cam slots to move the anvil and cartridge jaw between a spaced-apart position and a closed position in which the cartridge jaw and the anvil are configured to engage tissue therebetween, characterized in that the end effector also includes a closure mechanism proximal to the pivot point, the closure mechanism being positioned adjacent to a proximal-most end of the anvil and being configured to apply a force to the proximal-most end of the anvil to compress tissue engaged between the anvil and the cartridge jaw. an elongate shaft; and an end effector coupled to a distal end of the elongate shaft, the end effector including a cartridge jaw and an anvil pivotally coupled to one another at a pivot point, and a cam pin disposed within a cam slot formed in each of the cartridge jaw and the anvil, the cam pin being slidable within the cam slots to move the anvil and cartridge jaw between a spaced-apart position and a closed position in which the cartridge jaw and the anvil are configured to engage tissue therebetween, characterized in that the end effector also includes a closure mechanism proximal to the pivot point, the closure mechanism being positioned adjacent to a proximal-most end of the anvil and being configured to apply a force to the proximal-most end of the anvil to compress tissue engaged between the anvil and the cartridge jaw. a cartridge jaw and an anvil pivotally coupled to one another at a pivot point, and a cam pin disposed within a cam slot formed in each of the cartridge jaw and the anvil, the cam pin being slidable within the cam slots to move the anvil and cartridge jaw between a spaced-apart position and a closed position in which the cartridge jaw and the anvil are configured to engage tissue therebetween, characterized in that the end effector also includes a closure mechanism proximal to the pivot point, the closure mechanism being positioned adjacent to a proximal-most end of the anvil and being configured to apply a force to the proximal-most end of the anvil to compress tissue engaged between the anvil and the cartridge jaw.
Rail monitoring element with a carrier on which a strain sensor, in particular with an optical fiber with a fiber Bragg grating, is fastened, the carrier having an adhesive layer for adhesive fastening to a rail with a thermally activatable or thermally curable adhesive, characterized in that the adhesive layer comprises a heating element with contacts for the application of electrical energy.
Rotor or stator for an electric machine, which rotor or stator is arranged concentrically about a rotational axis, having a permanent magnet which is arranged in a cut-out of the rotor or stator and which has at least one elastic fixing means for fixing the permanent magnet, which elastic fixing means is arranged in the cut-out, the fixing means being a spring, characterized in that in each case one spacer element is provided between the springs and the permanent magnet, with the result that the springs do not touch the permanent magnet, the springs and the spacer elements being arranged in each case on axial fixing means which are configured as retaining washers, and the rotor or stator having the said axial fixing means which fix the permanent magnet in the cut-out in the axial direction, the axial fixing means being configured as retaining washers.
A floating structure for forming a floating barrier comprising at least one row of rectangular parallelepiped modular floating blocks, a block having four vertical peripheral faces meeting at four vertical edges, each vertical edge carrying an assembly lug in which an assembly system (8, 8') can be received in order to assemble said block to one or more adjacent blocks, said or each row comprising a plurality of blocks joined to one another by assembly systems (8, 8') arranged in two diagonally opposed assembly lugs of each block, apart from two end blocks, in which the floating structure is configured so that each block is connected to an adjacent block by a single connecting lug, characterised in that the floating structure comprises protective equipment extending in a longitudinal direction of the at least one row, and forming a protective surface of greater height than the height of the blocks, the protective equipment being fixed to a plurality of the assembly lugs of the blocks, the protective equipment comprising abutting flaps forming a "Y", the abutting flaps including two oblique flaps in the upper part and a substantially vertical flap in the lower part, the substantially vertical flap being provided with weights, each abutting flap comprising a waterproof tarpaulin and/or a net. said or each row comprising a plurality of blocks joined to one another by assembly systems (8, 8') arranged in two diagonally opposed assembly lugs of each block, apart from two end blocks, in which the floating structure is configured so that each block is connected to an adjacent block by a single connecting lug, characterised in that the floating structure comprises protective equipment extending in a longitudinal direction of the at least one row, and forming a protective surface of greater height than the height of the blocks, the protective equipment being fixed to a plurality of the assembly lugs of the blocks, the protective equipment comprising abutting flaps forming a "Y", the abutting flaps including two oblique flaps in the upper part and a substantially vertical flap in the lower part, the substantially vertical flap being provided with weights, each abutting flap comprising a waterproof tarpaulin and/or a net.
A method for loading a list of preferred networks into a secure element contained in a mobile terminal, characterized in that said loading is accomplished when the secure element detects that it is in a roaming condition within a telecommunication network, and in that it consists of: - i) asking for an IP channel to be opened between said telecommunication network and said secure element, with said request for opening originating with said secure element; - ii) requesting the loading of said list of preferred networks; - iii) uploading via said IP channel said list of preferred networks into said secure element; - iv) transmitting a request by said secure element to said mobile terminal to use one of said preferred networks in said list. - i) asking for an IP channel to be opened between said telecommunication network and said secure element, with said request for opening originating with said secure element; - ii) requesting the loading of said list of preferred networks; - iii) uploading via said IP channel said list of preferred networks into said secure element; - iv) transmitting a request by said secure element to said mobile terminal to use one of said preferred networks in said list.
A coupling of driving twin wheels for a drive axle of a vehicle in which there is included a fixed spindle, constrained to the supporting structure of the axle, on which a rotating element is rotatably coupled with a coaxial arrangement, with a rim of the first of said twin wheels firmly joined to the spindle, characterized in that the rim of the second of said driving twin wheels is firmly and coaxially joined to an element, which is retained axially in both directions, with respect to the rotating element, through thrust bearings (26, 27) and is in turn rotationally coupled, through a radial bearing, to an outer surface integral to the rotating element; braking means being provided which operate between the said element and a fixed annular element, that is integral with the spindle and is provided with a coaxially movable annular piston which acts, through the interposition of an axial bearing, against a movable annular element to activate said braking means; said coaxially movable annular piston being pushed, with a predetermined axial load, against the said movable annular element which is constrained in axially sliding manner, not to rotate with respect thereto, to a tubular body which, in turn, is made integral to the rotating element.
A process for preparing tauroursodeoxycholic acid having the following formula comprising the following stages: a) obtaining an aqueous suspension of an alkali or alkaline-earth salt of tauroursodeoxycholic acid; b) adding 0.8-1.4 equivalents of an acid, wherein said acid has a concentration in water of greater than 30% by weight; c) leaving the resultant suspension to stand for a period of between 10 and 180 minutes; d) filtering the suspension; e) adding an organic solvent with subsequent precipitation of the tauroursodeoxycholic acid. a) obtaining an aqueous suspension of an alkali or alkaline-earth salt of tauroursodeoxycholic acid; b) adding 0.8-1.4 equivalents of an acid, wherein said acid has a concentration in water of greater than 30% by weight; c) leaving the resultant suspension to stand for a period of between 10 and 180 minutes; d) filtering the suspension; e) adding an organic solvent with subsequent precipitation of the tauroursodeoxycholic acid.
Method for operating a jetting device, the jetting device being configured to expel droplets of an electrically conducting fluid wherein at least a part of the conductive fluid is positioned in a magnetic field, the method comprising the steps of: a) providing a direct electrical current in the part of the conductive fluid positioned in the magnetic field, thereby generating a Lorentz force in the conductive fluid and generating an amount of heat, and; b) providing an alternating electrical current in the part of the conductive fluid positioned in the magnetic field, the alternating electrical current generating an amount of heat; c) controlling the direct electrical current and the alternating electrical current such that the sum of heat generated by the direct electrical current and the alternating electrical current is substantially constant. a) providing a direct electrical current in the part of the conductive fluid positioned in the magnetic field, thereby generating a Lorentz force in the conductive fluid and generating an amount of heat, and; b) providing an alternating electrical current in the part of the conductive fluid positioned in the magnetic field, the alternating electrical current generating an amount of heat; c) controlling the direct electrical current and the alternating electrical current such that the sum of heat generated by the direct electrical current and the alternating electrical current is substantially constant.
A method performed by a wireless communication device for reducing handover delay, wherein the wireless communication device is arranged to operate in a cellular communication system and to operate in a cell using unlicensed spectrum, the method comprises: receiving (102, 201) a downlink, DL, signal from a first network node operating a neighbouring cell operating in the unlicensed spectrum, wherein the DL signal comprises a discovery reference signal, DRS, subframe, wherein the DRS is provided periodically to allow for initial cell detection and channel quality measurements for mobility and comprises synchronization signals and system information; and storing (104, 204) data associated with the DRS subframe, and then receiving a handover command from a second network node operating a serving cell where the neighbouring cell is a target cell; and performing a random access procedure for handover to the target cell when uplink listen-before-talk, LBT, succeeds, wherein the performing of the random access procedure is performed directly after the reception of the handover command based on the stored data without trying to receive the periodically provided target cell DRS. receiving (102, 201) a downlink, DL, signal from a first network node operating a neighbouring cell operating in the unlicensed spectrum, wherein the DL signal comprises a discovery reference signal, DRS, subframe, wherein the DRS is provided periodically to allow for initial cell detection and channel quality measurements for mobility and comprises synchronization signals and system information; and storing (104, 204) data associated with the DRS subframe, and then receiving a handover command from a second network node operating a serving cell where the neighbouring cell is a target cell; and performing a random access procedure for handover to the target cell when uplink listen-before-talk, LBT, succeeds, wherein the performing of the random access procedure is performed directly after the reception of the handover command based on the stored data without trying to receive the periodically provided target cell DRS.
An overhead conveying device (01, 30, 52, 55) for integration in an assembly plant in order to transport vehicle components by means of a conveying device arranged on the overhead conveying device (01, 30, 52, 55), said overhead conveying device having a supporting framework (03, 38), wherein the supporting framework (03, 38) has a length greater than 5 m and a width greater than 2 m, and wherein the conveying device can be mounted at the supporting framework (03, 38), and wherein the supporting framework (03, 38) and the conveying device form a conveying corridor, in which the vehicle components can be transported from an input point to an output point, and wherein the supporting framework (03, 38) is borne above the floor of the assembly plant by bearing means (39, 48), and wherein a free space, which is suitable for component assembly and/or for component transport and which can be walked and/or driven through, is formed between the underside of the supporting framework (03, 38) and the upper side of the floor by the bearing means (39, 48), wherein the supporting framework (03, 38) is formed from at least two supporting framework modules (03a, 03b, 03c, 38a, 38b) arranged one after another in the direction of the conveying corridor, characterised in that the supporting framework modules (03a, 03b, 03c, 38a, 38b) are designed in the manner of a spatial truss and comprise longitudinally and transversely oriented and diagonal and upright interconnected supporting beam elements (04, 05, 06, 07), wherein the supporting beam elements (04, 05, 06, 07) have a closed tubular cross-section.
A lumen reentry device comprising: a catheter comprising a balloon disposed at a distal end thereof, said catheter being formed with a first lumen through said balloon for passing therethrough a guidewire and a second lumen for inflation and deflation of said balloon, said catheter and said balloon being sized and shaped for entry between two layers (3, 4) of a wall of a blood vessel lumen, wherein upon expansion of said balloon when placed between said layers (3, 4), a distal portion of said first lumen that passes through said balloon is bent with respect to a portion of said first lumen proximal to said balloon and is directed towards said blood vessel lumen, characterized in that the balloon is conical and the distal end of the catheter does not protrude beyond said balloon.
Wireless charger for use in a vehicle, comprising a filter for filtering an electric field in a wireless charger for use in a vehicle, characterized by comprising: a first metallic filter structure (304, 305), arranged in a first metal layer of a printed circuit board and comprising multiple radial slits, configured to filter an electric field generated by a charging coil at the wireless charger arranged below the printed circuit board; a temperature sensor, configured to determine a temperature of a surface of the wireless charger; wherein the temperature sensor, is thermally coupled to the metallic filter structure (304, 305) and arranged outside the radial slits. a first metallic filter structure (304, 305), arranged in a first metal layer of a printed circuit board and comprising multiple radial slits, configured to filter an electric field generated by a charging coil at the wireless charger arranged below the printed circuit board; a temperature sensor, configured to determine a temperature of a surface of the wireless charger; wherein the temperature sensor, is thermally coupled to the metallic filter structure (304, 305) and arranged outside the radial slits.