mhurhangee/ep-ind-claims huggingface dataset:
An in situ method of determining a location of a target polynucleotide in a sample, comprising: (a) attaching the sample affixed to a support to a first microfluidic device having multiple first addressing channels, wherein a first addressing channel identifies a first area in the sample; (b) delivering a first probe through the first addressing channel to the first area in the sample, wherein the first probe comprises (i) a sequence that is at least 80% complementary to at least a portion of a target polynucleotide, (ii) a first address tag that identifies the first area in the sample, and (iii) a first ligation region; (c) attaching the sample affixed to the support to a second microfluidic device having multiple second addressing channels, wherein a second addressing channel identifies in the sample a second area that intersects with the first area at an angle greater than 0 degree, e.g., about 90 degrees, about 80 degrees, about 70 degrees, about 60 degrees, about 50 degrees, about 40 degrees, about 30 degrees, about 20 degrees, or about 10 degrees; and (d) delivering a second probe through the second addressing channel to the second area in the sample, wherein the second probe comprises (i) a second address tag that identifies the second area in the sample and (ii) a second ligation region, wherein the second probe is coupled to the first probe through ligation between the first and second ligation regions at an intersection between the first area and the second area, and the first and second address tags are used to identify the location of the target polynucleotide at the intersection in the sample. (a) attaching the sample affixed to a support to a first microfluidic device having multiple first addressing channels, wherein a first addressing channel identifies a first area in the sample; (b) delivering a first probe through the first addressing channel to the first area in the sample, wherein the first probe comprises (i) a sequence that is at least 80% complementary to at least a portion of a target polynucleotide, (ii) a first address tag that identifies the first area in the sample, and (iii) a first ligation region; (c) attaching the sample affixed to the support to a second microfluidic device having multiple second addressing channels, wherein a second addressing channel identifies in the sample a second area that intersects with the first area at an angle greater than 0 degree, e.g., about 90 degrees, about 80 degrees, about 70 degrees, about 60 degrees, about 50 degrees, about 40 degrees, about 30 degrees, about 20 degrees, or about 10 degrees; and (d) delivering a second probe through the second addressing channel to the second area in the sample, wherein the second probe comprises (i) a second address tag that identifies the second area in the sample and (ii) a second ligation region, wherein the second probe is coupled to the first probe through ligation between the first and second ligation regions at an intersection between the first area and the second area, and the first and second address tags are used to identify the location of the target polynucleotide at the intersection in the sample.
An ultrathin hermetic housing exhibiting a thickness of less than or equal to 3 mm, the ultrathin hermetic housing comprising: - a first element in the form of a sheet (11, 61), made of at least one material chosen from a metal, a ceramic and a glass; - a second element (15, 16; 65) having dimensions and a shape suitable for covering said first element, also made of at least one material chosen from a metal, a ceramic and a glass; - a first metal surround (12, 62) interposed between said first element and said second element, comprising an internal part (14, 64) positioned partially or completely inside the perimeter of said first element and an external part (13, 63) positioned completely outside said perimeter of said first element; - a first hermetic seal between said first element and said internal part of said first metal surround; and - a second hermetic seal between said second element and said external part of said first metal surround. - a first element in the form of a sheet (11, 61), made of at least one material chosen from a metal, a ceramic and a glass; - a second element (15, 16; 65) having dimensions and a shape suitable for covering said first element, also made of at least one material chosen from a metal, a ceramic and a glass; - a first metal surround (12, 62) interposed between said first element and said second element, comprising an internal part (14, 64) positioned partially or completely inside the perimeter of said first element and an external part (13, 63) positioned completely outside said perimeter of said first element; - a first hermetic seal between said first element and said internal part of said first metal surround; and - a second hermetic seal between said second element and said external part of said first metal surround.
Trim component for a motor vehicle, comprising - a wall which delimits an inner space of the trim component, - a plurality of through holes which penetrate the wall, and - a lighting device, arranged in the inner space of the trim component, which is connect to a light source and which comprises a light conductor, which is arranged in relation to the through holes such that the light emitted by the light conductor can flow through the through holes, characterized in that - the light conductor comprises, in the region of the through holes, decoupling sections, in which the light from the light conductor can emerge, wherein o the through holes comprise an extension which runs parallel to a longitudinal axis of the light conductor, wherein the extension increases as the distance of the through holes from the light source increases, and/or o each pair of adjacent through hole are arranged spaced at a distance to each other which extends parallel to the light conductor, wherein the distance decreases as the distance of the through holes from the light source increases. - a wall which delimits an inner space of the trim component, - a plurality of through holes which penetrate the wall, and - a lighting device, arranged in the inner space of the trim component, which is connect to a light source and which comprises a light conductor, which is arranged in relation to the through holes such that the light emitted by the light conductor can flow through the through holes, characterized in that - the light conductor comprises, in the region of the through holes, decoupling sections, in which the light from the light conductor can emerge, wherein o the through holes comprise an extension which runs parallel to a longitudinal axis of the light conductor, wherein the extension increases as the distance of the through holes from the light source increases, and/or o each pair of adjacent through hole are arranged spaced at a distance to each other which extends parallel to the light conductor, wherein the distance decreases as the distance of the through holes from the light source increases. o the through holes comprise an extension which runs parallel to a longitudinal axis of the light conductor, wherein the extension increases as the distance of the through holes from the light source increases, and/or o each pair of adjacent through hole are arranged spaced at a distance to each other which extends parallel to the light conductor, wherein the distance decreases as the distance of the through holes from the light source increases.
An optical amplifier that multiplexes pump lights having different wavelengths output from a plurality of pump light sources (3a, 3b) on a wavelength-division multiplexed signal light propagating through a transmission path to provide Raman amplification, comprising: an intensity modulator that modulates intensity of a pump light to be output from any one of the pump light sources (3a, 3b), at a predetermined frequency; a photoelectric converter that converts a part of the wavelength-division multiplexed signal light to electrical signals; a gain modulation signal detector that is connected to the photoelectric converter and extracts a gain modulation signal having a component of the frequency from the electrical signals output from the photoelectrical converter; and a controller that is connected to the gain modulation signal detector, wherein the output of the plurality of pump light sources (3a, 3b) is coupled to the transmission path, and a part of the wavelength-division multiplexed signal light, which is Raman-amplified and propagates through the transmission path, is branched to the photoelectric converter, and the controller functions as the intensity modulator and controls only the intensity of pump lights that are output from the pump light source (3a, 3b), of which intensity is modulated at the frequency to control Raman amplification gain so that an amplitude of the gain modulation signal becomes a predetermined value, characterized in that the intensities of pump lights output from individual pump light sources (3a, 3b) are sequentially controlled in order from a pump light having higher intensity to obtain a predetermined value of the Raman amplification gain. an intensity modulator that modulates intensity of a pump light to be output from any one of the pump light sources (3a, 3b), at a predetermined frequency; a photoelectric converter that converts a part of the wavelength-division multiplexed signal light to electrical signals; a gain modulation signal detector that is connected to the photoelectric converter and extracts a gain modulation signal having a component of the frequency from the electrical signals output from the photoelectrical converter; and a controller that is connected to the gain modulation signal detector, wherein the output of the plurality of pump light sources (3a, 3b) is coupled to the transmission path, and a part of the wavelength-division multiplexed signal light, which is Raman-amplified and propagates through the transmission path, is branched to the photoelectric converter, and the controller functions as the intensity modulator and controls only the intensity of pump lights that are output from the pump light source (3a, 3b), of which intensity is modulated at the frequency to control Raman amplification gain so that an amplitude of the gain modulation signal becomes a predetermined value, characterized in that the intensities of pump lights output from individual pump light sources (3a, 3b) are sequentially controlled in order from a pump light having higher intensity to obtain a predetermined value of the Raman amplification gain.
A suitcase comprising a case body, the case body being provided with rolling wheels and a pull rod, wherein a foldable display screen is provided on an outer surface of the case body, the display screen comprises a fixed first display unit and at least one second display unit which is foldable relative to the first display unit, when the display screen is in the folded state, the at least one second display unit is folded on and overlapped with the first display unit, after the at least one second display unit is unfolded relative to the first display unit, the display screen is in the unfolded state, and the display area of the display screen is equal to the sum of the first display unit and the at least one second display unit; the at least one second display unit is a single one, the second display unit is rotatably connected with a side edge of the first display unit, and in the unfolded state, the second display unit is located at one side of the first display unit; the display area of the first display unit wholly covers the outer surface of the case body on which the display screen is provided, and the display area of the second display unit is equal to that of the first display unit; a plurality of support bars are provided on the case body, and each support bar is rotatably connected to the case body, the support bars are configured to rotate outwards from the case body to support the back portion of the unfolded second display unit when the second display unit is in the unfolded state, and configured to rotate back to the case body when the second display unit is in the folded state. the at least one second display unit is a single one, the second display unit is rotatably connected with a side edge of the first display unit, and in the unfolded state, the second display unit is located at one side of the first display unit; the display area of the first display unit wholly covers the outer surface of the case body on which the display screen is provided, and the display area of the second display unit is equal to that of the first display unit; a plurality of support bars are provided on the case body, and each support bar is rotatably connected to the case body, the support bars are configured to rotate outwards from the case body to support the back portion of the unfolded second display unit when the second display unit is in the unfolded state, and configured to rotate back to the case body when the second display unit is in the folded state.
A yarn feeder for textile machines, comprising a housing supporting a yarn-winding drum which is driven to rotate by a motor controlled by an electronic circuit as a function of signals from a tension sensor integral to the housing, said tension sensor comprising a flexible member arranged to interfere with the yarn unwinding from said yarn-winding drum and to bend to a variable extent depending on the yarn tension, said flexible member incorporating circuit means which generate a variable output depending on the bent configuration of the flexible member, characterized in that said housing has a first peripheral surface and an opposed, second peripheral surface which have mutually engageable profiles and are provided with respective male/female connectors (52, 54) for mechanical/electrical connection of a plurality of identical feeders in a stacked configuration, said electronic circuit being programmed to enable said motor to unwind yarn in response to a deformation of said flexible member with respect to a resting configuration corresponding to a predetermined, minimum tension, on the basis of setup instruction transferred via said connectors (52, 54).
A method of manufacturing a fan blade for a gas turbine engine, comprising: positioning a first member against a second member, the first member having a first surface and the second member having a second surface, each of the first surface and the second surface extending from a leading edge to a trailing edge of the fan blade and from a tip edge to or proximate to a root of the fan blade, wherein the second member includes structural ribs extending to the first member; and bonding the second member to the first member at free edges of the structural ribs and about a perimeter of the fan blade, the first member being oversized so that the bonding between the first member and the second member results in skin extensions (144, 144a, 144b, 144c) around the blade perimeter, wherein the skin extensions (144, 144a, 144b, 144c) extend past the second member at the leading edge, the trailing edge, the tip and the root, whereby the bonding occurs away from the edges of the first member; and removing the skin extensions after bonding. positioning a first member against a second member, the first member having a first surface and the second member having a second surface, each of the first surface and the second surface extending from a leading edge to a trailing edge of the fan blade and from a tip edge to or proximate to a root of the fan blade, wherein the second member includes structural ribs extending to the first member; and bonding the second member to the first member at free edges of the structural ribs and about a perimeter of the fan blade, the first member being oversized so that the bonding between the first member and the second member results in skin extensions (144, 144a, 144b, 144c) around the blade perimeter, wherein the skin extensions (144, 144a, 144b, 144c) extend past the second member at the leading edge, the trailing edge, the tip and the root, whereby the bonding occurs away from the edges of the first member; and removing the skin extensions after bonding.
A grommet comprising: a tapered barrel part including a large diameter cylindrical portion, a small diameter cylindrical portion, and an elastic deformation portion disposed between the large diameter cylindrical portion and the small diameter cylindrical portion; wherein a fitting groove to be fitted in a through hole of a panel, is formed on the large diameter cylindrical portion, and wherein a thick walled portion is formed on an inner surface of the elastic deformation portion; and a tape winding spatula part connected to the small diameter cylindrical portion and holding a wire harness passed through therein. a tapered barrel part including a large diameter cylindrical portion, a small diameter cylindrical portion, and an elastic deformation portion disposed between the large diameter cylindrical portion and the small diameter cylindrical portion; wherein a fitting groove to be fitted in a through hole of a panel, is formed on the large diameter cylindrical portion, and wherein a thick walled portion is formed on an inner surface of the elastic deformation portion; and a tape winding spatula part connected to the small diameter cylindrical portion and holding a wire harness passed through therein.
A system, comprising: a turbine combustor, comprising: a housing; a combustion chamber; a liner disposed within the housing and defining the combustion chamber; a flow sleeve disposed within the housing and disposed about the liner; an extraction outlet; a first volume configured to receive a combustion fluid and to direct the combustion fluid into the combustion chamber, wherein the first volume is configured to direct at least some of the combustion fluid into the combustion chamber in a radial direction via one or more first combustion fluid openings in the flow sleeve and one or more second combustion fluid openings in the liner, a second volume configured to receive a first flow of an exhaust gas and to direct the first flow of the exhaust gas into the combustion chamber; and a third volume defined between the housing and an aft portion of the flow sleeve and disposed axially downstream from the first volume and circumferentially about at least a portion of the second volume, wherein the third volume is configured to receive a second flow of the exhaust gas and to direct the second flow of the exhaust gas out of the turbine combustor via the extraction outlet, the third volume is isolated from each of the first volume and the second volume, and the third volume is isolated from the first volume by a flange that extends radially outward from the flow sleeve to the housing. a housing; a combustion chamber; a liner disposed within the housing and defining the combustion chamber; a flow sleeve disposed within the housing and disposed about the liner; an extraction outlet; a first volume configured to receive a combustion fluid and to direct the combustion fluid into the combustion chamber, wherein the first volume is configured to direct at least some of the combustion fluid into the combustion chamber in a radial direction via one or more first combustion fluid openings in the flow sleeve and one or more second combustion fluid openings in the liner, a second volume configured to receive a first flow of an exhaust gas and to direct the first flow of the exhaust gas into the combustion chamber; and a third volume defined between the housing and an aft portion of the flow sleeve and disposed axially downstream from the first volume and circumferentially about at least a portion of the second volume, wherein the third volume is configured to receive a second flow of the exhaust gas and to direct the second flow of the exhaust gas out of the turbine combustor via the extraction outlet, the third volume is isolated from each of the first volume and the second volume, and the third volume is isolated from the first volume by a flange that extends radially outward from the flow sleeve to the housing.
Apparatus for thermocoagulation of body tissue by means of laser radiation with - at least two laser beam sources (2, 4) which emit laser radiation having different central wavelengths towards a processing surface, - a device for superimposing the laser radiation of the laser beam sources (2,4), and - a control device for controlling the laser power of the respective laser beam sources (2, 4), which actuates the laser beam sources (2, 4) in a processing mode of the apparatus for the simultaneous emission of the laser radiation in order to carry out a thermocoagulation process, characterized in that the apparatus is equipped with a device for detecting backscatter signals from the processing surface as process signals, wherein the device for detecting backscatter signals detects the backscatter signals in spectrally resolved manner or detects the backscatter signals separately from each other in two different spectral ranges, and the control device is designed such that it changes the ratio of the laser powers of the laser beam sources (2, 4) dynamically in the processing mode depending on the process signal of the detected backscatter signals detected from the thermocoagulation process based on a spectral analysis of the backscatter or based on the strength of the backscatter measured in each spectral range. - at least two laser beam sources (2, 4) which emit laser radiation having different central wavelengths towards a processing surface, - a device for superimposing the laser radiation of the laser beam sources (2,4), and - a control device for controlling the laser power of the respective laser beam sources (2, 4), which actuates the laser beam sources (2, 4) in a processing mode of the apparatus for the simultaneous emission of the laser radiation in order to carry out a thermocoagulation process, characterized in that the apparatus is equipped with a device for detecting backscatter signals from the processing surface as process signals, wherein the device for detecting backscatter signals detects the backscatter signals in spectrally resolved manner or detects the backscatter signals separately from each other in two different spectral ranges, and the control device is designed such that it changes the ratio of the laser powers of the laser beam sources (2, 4) dynamically in the processing mode depending on the process signal of the detected backscatter signals detected from the thermocoagulation process based on a spectral analysis of the backscatter or based on the strength of the backscatter measured in each spectral range.
A method for measuring the level of Her2 protein in a protein digest prepared from a biological sample of formalin-fixed tissue, comprising detecting and quantifying a peptide of Her2 in said protein digest prepared from said biological sample using mass spectrometry; and calculating the level of Her2 protein in said sample, wherein said peptide is the peptide of SEQ ID NO:7 and wherein said level is an absolute level.