A microfluidic device for concentrating and detecting particles contained in a fluid sample, comprising a substrate configured for being set in rotation with respect to a centre of rotation, the substrate having a surface at which at least one microfluidic arrangement is defined, which extends substantially according to a plane identified by the substrate, wherein the at least one microfluidic arrangement comprises a plurality of microchannels each having a first end and a second end, wherein: - each microchannel of the plurality comprises, in a region thereof intermediate to its first end and its second end, at least one detection area that is at a first distance in a radial direction with respect to the centre of rotation of the substrate; - the first end and the second end of each microchannel of the plurality are at second distances in a radial direction with respect to the centre of rotation of the substrate, said second distances being preferably the same as one another; - the first distance in a radial direction is greater than the second distances in a radial direction, wherein: - the detection area is an area for particle accumulation, - the at least one microfluidic arrangement comprises at least one first duct or chamber and a second duct or chamber, the plurality of microchannels having the respective first ends and second ends connected in parallel to the first duct or chamber and to the second duct or chamber, respectively, one of the first duct or chamber and second duct or chamber being pre-arranged for receiving the fluid sample,, - in the at least one detection area for particle accumulation, each microchannel of the plurality is substantially V-shaped or U-shaped, or comprises two substantially convergent microchannel branches (13c, 13d), and - each microchannel of the plurality is open only at its first end and its second end, in such a way that particles contained in a volume of fluid of the fluid sample that penetrates into at least one microchannel of the plurality concentrate in the at least one detection area as a result of the centrifugal force caused by a rotation of the substrate about the centre of rotation. - each microchannel of the plurality comprises, in a region thereof intermediate to its first end and its second end, at least one detection area that is at a first distance in a radial direction with respect to the centre of rotation of the substrate; - the first end and the second end of each microchannel of the plurality are at second distances in a radial direction with respect to the centre of rotation of the substrate, said second distances being preferably the same as one another; - the first distance in a radial direction is greater than the second distances in a radial direction, - the detection area is an area for particle accumulation, - the at least one microfluidic arrangement comprises at least one first duct or chamber and a second duct or chamber, the plurality of microchannels having the respective first ends and second ends connected in parallel to the first duct or chamber and to the second duct or chamber, respectively, one of the first duct or chamber and second duct or chamber being pre-arranged for receiving the fluid sample,, - in the at least one detection area for particle accumulation, each microchannel of the plurality is substantially V-shaped or U-shaped, or comprises two substantially convergent microchannel branches (13c, 13d), and - each microchannel of the plurality is open only at its first end and its second end, in such a way that particles contained in a volume of fluid of the fluid sample that penetrates into at least one microchannel of the plurality concentrate in the at least one detection area as a result of the centrifugal force caused by a rotation of the substrate about the centre of rotation.