A method for specifically detecting Plasmodium species nucleic acid in a sample, said method comprising: contacting a sample, said sample suspected of containing Plasmodium species nucleic acid, with at least two oligomers for amplifying a target region of a Plasmodium species target nucleic acid, wherein the at least two amplification oligomers comprise: (a) an amplification oligomer comprising a target-hybridizing sequence (i) that is from 14 to 20 contiguous nucleotides in length, is contained in the sequence of SEQ ID NO: 162, and comprises the sequence of SEQ ID NO: 163; or (ii) that is from 14 to 25 contiguous nucleotides in length, is contained in the sequence of SEQ ID NO: 166, and comprises the sequence of SEQ ID NO: 167 or SEQ ID NO: 168; and (b) an amplification oligomer comprising a target-hybridizing sequence that is from 15 to 33 contiguous nucleotides in length, is contained in SEQ ID NO: 169 and comprises the sequence of SEQ ID NO: 171, SEQ ID NO: 172, or SEQ ID NO: 173; performing an in vitro nucleic acid amplification reaction, wherein any Plasmodium target nucleic acid present in said sample is used as a template for generating an amplification product; and detecting the presence or absence of the amplification product, thereby indicating the presence or absence of Plasmodium species target nucleic acid in said sample. contacting a sample, said sample suspected of containing Plasmodium species nucleic acid, with at least two oligomers for amplifying a target region of a Plasmodium species target nucleic acid, wherein the at least two amplification oligomers comprise: (a) an amplification oligomer comprising a target-hybridizing sequence (i) that is from 14 to 20 contiguous nucleotides in length, is contained in the sequence of SEQ ID NO: 162, and comprises the sequence of SEQ ID NO: 163; or (ii) that is from 14 to 25 contiguous nucleotides in length, is contained in the sequence of SEQ ID NO: 166, and comprises the sequence of SEQ ID NO: 167 or SEQ ID NO: 168; and (b) an amplification oligomer comprising a target-hybridizing sequence that is from 15 to 33 contiguous nucleotides in length, is contained in SEQ ID NO: 169 and comprises the sequence of SEQ ID NO: 171, SEQ ID NO: 172, or SEQ ID NO: 173; (a) an amplification oligomer comprising a target-hybridizing sequence (i) that is from 14 to 20 contiguous nucleotides in length, is contained in the sequence of SEQ ID NO: 162, and comprises the sequence of SEQ ID NO: 163; or (ii) that is from 14 to 25 contiguous nucleotides in length, is contained in the sequence of SEQ ID NO: 166, and comprises the sequence of SEQ ID NO: 167 or SEQ ID NO: 168; and (i) that is from 14 to 20 contiguous nucleotides in length, is contained in the sequence of SEQ ID NO: 162, and comprises the sequence of SEQ ID NO: 163; or (ii) that is from 14 to 25 contiguous nucleotides in length, is contained in the sequence of SEQ ID NO: 166, and comprises the sequence of SEQ ID NO: 167 or SEQ ID NO: 168; and (b) an amplification oligomer comprising a target-hybridizing sequence that is from 15 to 33 contiguous nucleotides in length, is contained in SEQ ID NO: 169 and comprises the sequence of SEQ ID NO: 171, SEQ ID NO: 172, or SEQ ID NO: 173; performing an in vitro nucleic acid amplification reaction, wherein any Plasmodium target nucleic acid present in said sample is used as a template for generating an amplification product; and detecting the presence or absence of the amplification product, thereby indicating the presence or absence of Plasmodium species target nucleic acid in said sample.