A mobile microscopic imaging device wherein the mobile microscopic imaging device is based on a transmission fluorescence mode and comprises - a sample stage for holding a sample to be imaged; - at least one excitation light source which is positional to the optical axis of the transmission fluorescence mode of the device adjacent to the sample stage, the at least one excitation light source being configured to emit non-collimated excitation light in order to illuminate an imaged area for illuminating the sample on a surface plane of the sample stage; - an imaging panel configured to capture at least one image of the imaged area for imaging the sample with fluorescent light generated by the sample in illumination of the excitation light; - an optical magnification unit between the sample and the imaging panel for guiding light from the illuminated sample to the imaging panel for forming a magnified image of at least one portion of the sample at the imaging panel, said optical magnification unit comprising at least one polymeric lens, characterized in that the mobile microscope imaging device further comprises - at least one stacked filter which comprises a dielectric filter and an absorption filter, transmission of the absorption filter being based on gelatin material, and the dielectric filter being located between the absorption filter and the sample stage for the dielectric filter to reflect the excitation light towards the imaged area; and - the stacked filter is located between the surface plane of the sample stage and the at least one polymeric lens, and the at least one stacked filter is configured to prevent the non-collimated excitation light of the at least one excitation light source from propagating to the imaging panel in order to avoid the generation of light of auto fluorescence of the at least one polymeric lens and the gelatin material of the absorption filter. - a sample stage for holding a sample to be imaged; - at least one excitation light source which is positional to the optical axis of the transmission fluorescence mode of the device adjacent to the sample stage, the at least one excitation light source being configured to emit non-collimated excitation light in order to illuminate an imaged area for illuminating the sample on a surface plane of the sample stage; - an imaging panel configured to capture at least one image of the imaged area for imaging the sample with fluorescent light generated by the sample in illumination of the excitation light; - an optical magnification unit between the sample and the imaging panel for guiding light from the illuminated sample to the imaging panel for forming a magnified image of at least one portion of the sample at the imaging panel, said optical magnification unit comprising at least one polymeric lens, characterized in that the mobile microscope imaging device further comprises - at least one stacked filter which comprises a dielectric filter and an absorption filter, transmission of the absorption filter being based on gelatin material, and the dielectric filter being located between the absorption filter and the sample stage for the dielectric filter to reflect the excitation light towards the imaged area; and - the stacked filter is located between the surface plane of the sample stage and the at least one polymeric lens, and the at least one stacked filter is configured to prevent the non-collimated excitation light of the at least one excitation light source from propagating to the imaging panel in order to avoid the generation of light of auto fluorescence of the at least one polymeric lens and the gelatin material of the absorption filter.