An integrated single-use device for performing a nucleic acid analysis, comprising: a housing with an entry port for accepting a sample suspected of containing a target nucleic acid, a first chamber connected to the entry port and comprising: a reagent for extracting the target nucleic acid, magnetic beads comprising a coating configured to bind the extracted target nucleic acid, and a wax which can melt to form a contiguous wax filtering layer in a region of egress from the first chamber to a first conduit, wherein the magnetic beads binding the extracted target nucleic acid may pass through the wax layer into the first conduit by means of an applied magnetic field, the first conduit connected to the first chamber and an amplification chamber, to permit passage of the extracted target nucleic acid into the amplification chamber, the amplification chamber having a heating means and a temperature sensing means for controlling amplification conditions to amplify the extracted target nucleic acid into an amplified target nucleic acid, wherein the first conduit, the amplification chamber, or alternatively a holding chamber contain an amplification reagent that is capable of incorporating a detectable label into the amplified target nucleic acid, a sensing region comprising an immobilized capture oligonucleotide and a sensor, a second conduit connected to the amplification chamber and comprising the sensing region, a pump means connected to the amplification chamber for moving the amplified target nucleic acid through the second conduit to the sensing region to permit binding of said amplified target nucleic acid to said immobilized capture oligonucleotide, a waste chamber connected to the second conduit, and a holding chamber containing a detection reagent connected via a third conduit to the second conduit between an egress of the amplification chamber and the sensing region, the detection reagent being able to substantially displace uncaptured amplified target from the sensing region to the waste chamber and permit sensing of said detectable label retained in said sensing regions. a housing with an entry port for accepting a sample suspected of containing a target nucleic acid, a first chamber connected to the entry port and comprising: a reagent for extracting the target nucleic acid, magnetic beads comprising a coating configured to bind the extracted target nucleic acid, and a wax which can melt to form a contiguous wax filtering layer in a region of egress from the first chamber to a first conduit, wherein the magnetic beads binding the extracted target nucleic acid may pass through the wax layer into the first conduit by means of an applied magnetic field, a reagent for extracting the target nucleic acid, magnetic beads comprising a coating configured to bind the extracted target nucleic acid, and a wax which can melt to form a contiguous wax filtering layer in a region of egress from the first chamber to a first conduit, wherein the magnetic beads binding the extracted target nucleic acid may pass through the wax layer into the first conduit by means of an applied magnetic field, the first conduit connected to the first chamber and an amplification chamber, to permit passage of the extracted target nucleic acid into the amplification chamber, the amplification chamber having a heating means and a temperature sensing means for controlling amplification conditions to amplify the extracted target nucleic acid into an amplified target nucleic acid, wherein the first conduit, the amplification chamber, or alternatively a holding chamber contain an amplification reagent that is capable of incorporating a detectable label into the amplified target nucleic acid, a sensing region comprising an immobilized capture oligonucleotide and a sensor, a second conduit connected to the amplification chamber and comprising the sensing region, a pump means connected to the amplification chamber for moving the amplified target nucleic acid through the second conduit to the sensing region to permit binding of said amplified target nucleic acid to said immobilized capture oligonucleotide, a waste chamber connected to the second conduit, and a holding chamber containing a detection reagent connected via a third conduit to the second conduit between an egress of the amplification chamber and the sensing region, the detection reagent being able to substantially displace uncaptured amplified target from the sensing region to the waste chamber and permit sensing of said detectable label retained in said sensing regions.