A waterproof treatment method for a splicing part of floorboards, comprising: applying a waterproof wax to splicing parts of two floorboards matched with each other; and coating an anti-water agent on a surface of the two floorboards after spliced with each other; wherein the waterproof wax is applied in an amount of 1.35-1.45 g/m 2; the waterproof wax comprises 60-85% by weight of a first paraffin, 1-15% by weight of a silicone resin, 10-20% by weight of ethylene glycol dimethacrylate, 2-5% by weight of amino silicone oil and 0.1-1% by weight of alkenyl succinate; a side surface of one of the two floorboards is provided with a female groove; a side surface of the other one of the two floorboards is provided with a male groove; the male groove is matched with the female groove; and the waterproof wax is applied between the female groove and the male groove; the anti-water agent is prepared from 30-80% by weight of polyurethane acrylate, 10-60% by weight of diphenylmethane diisocyanate, 1-5% by weight of chlorinated paraffin, 1-2% by weight of a first curing agent, 1-2% by weight of a coupling agent and 0.2-1% by weight of a film-forming aid; the anti-water agent is coated in an amount of 0.45-0.55 g/m 2, wherein the first curing agent is diethylenetriamine; the coupling agent is a silane coupling agent; and the film forming aid is 2,2,4-trimethyl-1,3-pentanediol monoisobutyrate; main bodies of the two floorboards are both made of a waterproof substrate; the waterproof substrate is prepared from timber, an adhesive, a second paraffin, a waterproof agent and a second curing agent in a weight ratio of 700-1000:250-400:5-15:1-2:1-2; the adhesive is a melamine-modified resin; the waterproof agent is a paraffin emulsion; and the second curing agent is ammonium chloride. applying a waterproof wax to splicing parts of two floorboards matched with each other; and coating an anti-water agent on a surface of the two floorboards after spliced with each other; wherein the waterproof wax is applied in an amount of 1.35-1.45 g/m 2; the waterproof wax comprises 60-85% by weight of a first paraffin, 1-15% by weight of a silicone resin, 10-20% by weight of ethylene glycol dimethacrylate, 2-5% by weight of amino silicone oil and 0.1-1% by weight of alkenyl succinate; a side surface of one of the two floorboards is provided with a female groove; a side surface of the other one of the two floorboards is provided with a male groove; the male groove is matched with the female groove; and the waterproof wax is applied between the female groove and the male groove; the anti-water agent is prepared from 30-80% by weight of polyurethane acrylate, 10-60% by weight of diphenylmethane diisocyanate, 1-5% by weight of chlorinated paraffin, 1-2% by weight of a first curing agent, 1-2% by weight of a coupling agent and 0.2-1% by weight of a film-forming aid; the anti-water agent is coated in an amount of 0.45-0.55 g/m 2, wherein the first curing agent is diethylenetriamine; the coupling agent is a silane coupling agent; and the film forming aid is 2,2,4-trimethyl-1,3-pentanediol monoisobutyrate; main bodies of the two floorboards are both made of a waterproof substrate; the waterproof substrate is prepared from timber, an adhesive, a second paraffin, a waterproof agent and a second curing agent in a weight ratio of 700-1000:250-400:5-15:1-2:1-2; the adhesive is a melamine-modified resin; the waterproof agent is a paraffin emulsion; and the second curing agent is ammonium chloride.