Extraction system for extracting microorganisms contained in a sample, said extraction system comprising: - a support bar comprising: - a sample well intended to receive a sample containing microorganisms, such as a positive blood culture, - a reagent well intended to contain a selective lysis buffer, - at least one washing well (6.1) intended to contain a washing reagent, and - at least one protection and retention housing (9, 9.1, 9.2), - a pipetting element removably mounted on the support bar and extending at least partially into the at least one protection and retention housing, - a filtering and transfer device intended to capture and accumulate microorganisms contained in the sample and transfer the captured and accumulated microorganisms, the filtering and transfer device being removably mounted on the support bar and extending at least partially into the at least one protection and retention housing, said filtering and transfer device comprising: - a hollow body having an elongated shape and comprising a proximal end portion (12.1) and a distal end portion (12.2), and - a filtering element fixed to the distal end portion (12.2) of the hollow body, the filtering element having a cylindrical shape with a diameter adapted to the diameter of a spot of a MALDI-TOF MS analysis plate, and having a flat or hemispherical distal portion. - a support bar comprising: - a sample well intended to receive a sample containing microorganisms, such as a positive blood culture, - a reagent well intended to contain a selective lysis buffer, - at least one washing well (6.1) intended to contain a washing reagent, and - at least one protection and retention housing (9, 9.1, 9.2), - a sample well intended to receive a sample containing microorganisms, such as a positive blood culture, - a reagent well intended to contain a selective lysis buffer, - at least one washing well (6.1) intended to contain a washing reagent, and - at least one protection and retention housing (9, 9.1, 9.2), - a pipetting element removably mounted on the support bar and extending at least partially into the at least one protection and retention housing, - a filtering and transfer device intended to capture and accumulate microorganisms contained in the sample and transfer the captured and accumulated microorganisms, the filtering and transfer device being removably mounted on the support bar and extending at least partially into the at least one protection and retention housing, said filtering and transfer device comprising: - a hollow body having an elongated shape and comprising a proximal end portion (12.1) and a distal end portion (12.2), and - a filtering element fixed to the distal end portion (12.2) of the hollow body, the filtering element having a cylindrical shape with a diameter adapted to the diameter of a spot of a MALDI-TOF MS analysis plate, and having a flat or hemispherical distal portion. - a hollow body having an elongated shape and comprising a proximal end portion (12.1) and a distal end portion (12.2), and - a filtering element fixed to the distal end portion (12.2) of the hollow body, the filtering element having a cylindrical shape with a diameter adapted to the diameter of a spot of a MALDI-TOF MS analysis plate, and having a flat or hemispherical distal portion.