Method for evaluating a luminescence signal in an analyser for analysing a sample of biological material, wherein the method comprises the following steps: reading the luminescence signal from an interface to at least one optical readout unit of the analyser, wherein the luminescence signal represents intensity values for a luminescence of the sample arranged in a microfluidic apparatus with at least one sought wavelength, wherein the microfluidic apparatus is coupled to the analyser for processing the sample in a plurality of processing cycles; processing the luminescence signal using an optimization process in order to provide analysis data which are optimized with respect to a signal distortion of the luminescence signal, wherein the optimization process comprises an intensity correction of the intensity values represented by the luminescence signal for all processing cycles and sought wavelengths of the luminescence using in each case one correction factor, valid for all sought wavelengths and all intensity values, per processing cycle in order to generate corrected intensity values, wherein each correction factor takes into account or represents a signal distortion of the luminescence signal to be corrected, which is dependent on the processing of the sample, for the respective intensity value, wherein the optimization process comprises a determination, carried out in parallel with the intensity correction, per one sigmoid function with expected intensity values per sought wavelength, wherein the optimization process is carried out iteratively, wherein each correction factor is optimized during each iteration using an optimization algorithm, in particular wherein the optimization algorithm comprises an estimation according to the least squares method, wherein the correction factors are predefined ideal values and/or are estimated using the luminescence signal by means of an estimation rule, in particular wherein the estimation rule comprises an ascertainment of a portion of each intensity value for each processing cycle relative to an average value for each sought wavelength, a potentiation of the portion with a weighting factor, and the generation of an arithmetic mean over all sought wavelengths; and classifying the luminescence signal into one of a plurality of classes using the analysis data, with each class representing one analysis result (454, 456) of the sample. reading the luminescence signal from an interface to at least one optical readout unit of the analyser, wherein the luminescence signal represents intensity values for a luminescence of the sample arranged in a microfluidic apparatus with at least one sought wavelength, wherein the microfluidic apparatus is coupled to the analyser for processing the sample in a plurality of processing cycles; processing the luminescence signal using an optimization process in order to provide analysis data which are optimized with respect to a signal distortion of the luminescence signal, wherein the optimization process comprises an intensity correction of the intensity values represented by the luminescence signal for all processing cycles and sought wavelengths of the luminescence using in each case one correction factor, valid for all sought wavelengths and all intensity values, per processing cycle in order to generate corrected intensity values, wherein each correction factor takes into account or represents a signal distortion of the luminescence signal to be corrected, which is dependent on the processing of the sample, for the respective intensity value, wherein the optimization process comprises a determination, carried out in parallel with the intensity correction, per one sigmoid function with expected intensity values per sought wavelength, wherein the optimization process is carried out iteratively, wherein each correction factor is optimized during each iteration using an optimization algorithm, in particular wherein the optimization algorithm comprises an estimation according to the least squares method, wherein the correction factors are predefined ideal values and/or are estimated using the luminescence signal by means of an estimation rule, in particular wherein the estimation rule comprises an ascertainment of a portion of each intensity value for each processing cycle relative to an average value for each sought wavelength, a potentiation of the portion with a weighting factor, and the generation of an arithmetic mean over all sought wavelengths; and classifying the luminescence signal into one of a plurality of classes using the analysis data, with each class representing one analysis result (454, 456) of the sample.