A method for evaluating fertility potential of a living sperm cell, the method comprising: providing a classifier of the sperm cell's fertility potential based on the sperm cell's DNA fragmentation level, said classifier being defined by a classification function being a correlation function correlating between one or more predetermined physio spatial parameters of sperm cells and DNA fragmentation distribution in sperm cells, said classification function being predetermined based on a dataset of training sperm cell samples each characterized by measured one or more physio spatial parameters and respective DNA fragmentation levels derived from a DNA fragmentation assay; providing input data to the classifier indicative of the one or more predetermined physio spatial parameters of the living sperm cell being inspected, said one or more predetermined physio spatial parameters being obtained from at least one of two-dimensional and three-dimensional image data of the sperm cell, wherein each of said one or more physio spatial parameters comprises one or more of the following: surface area of the living sperm cell or an organelle thereof; dry mass of the living sperm cell or of an organelle thereof; an average optical path delay of the living sperm cell or of an organelle thereof; a variance of optical path delay being calculated of surface area of the living sperm cell or of an organelle thereof; a subtraction between an average optical path delay calculated upon an anterior portion of the head of the living sperm cell and an average optical path delay calculated upon a posterior portion of the head of the living sperm cell, each of said anterior and posterior portions comprising between 40 and 60 percent of the surface area of the head of the living sperm cell; and applying said classification function to said one or more predetermined physio spatial parameters of the living sperm cell being inspected and determining a DNA fragmentation level, inside said DNA fragmentation distribution, in said living sperm cell being inspected, the DNA fragmentation level being indicative of the fertility potential of the living sperm cell. providing a classifier of the sperm cell's fertility potential based on the sperm cell's DNA fragmentation level, said classifier being defined by a classification function being a correlation function correlating between one or more predetermined physio spatial parameters of sperm cells and DNA fragmentation distribution in sperm cells, said classification function being predetermined based on a dataset of training sperm cell samples each characterized by measured one or more physio spatial parameters and respective DNA fragmentation levels derived from a DNA fragmentation assay; providing input data to the classifier indicative of the one or more predetermined physio spatial parameters of the living sperm cell being inspected, said one or more predetermined physio spatial parameters being obtained from at least one of two-dimensional and three-dimensional image data of the sperm cell, wherein each of said one or more physio spatial parameters comprises one or more of the following: surface area of the living sperm cell or an organelle thereof; dry mass of the living sperm cell or of an organelle thereof; an average optical path delay of the living sperm cell or of an organelle thereof; a variance of optical path delay being calculated of surface area of the living sperm cell or of an organelle thereof; a subtraction between an average optical path delay calculated upon an anterior portion of the head of the living sperm cell and an average optical path delay calculated upon a posterior portion of the head of the living sperm cell, each of said anterior and posterior portions comprising between 40 and 60 percent of the surface area of the head of the living sperm cell; and applying said classification function to said one or more predetermined physio spatial parameters of the living sperm cell being inspected and determining a DNA fragmentation level, inside said DNA fragmentation distribution, in said living sperm cell being inspected, the DNA fragmentation level being indicative of the fertility potential of the living sperm cell.