Method for analyzing one or more single cells of interest, comprising - provision of i. a porous membrane comprising at least one pore, wherein the pore is adjusted to trap a single cell of interest when the cell is applied to the membrane in a liquid medium and to allow smaller or more flexible cells of the cell suspension to pass through the pore, ii. a well plate comprising at least one well, wherein the well diameter is larger than the pore diameter and the at least one well contains reagents for analyzing at least one component of the cells of interest, wherein the reagents can be provided in liquid or dry form, iii. means for assembling the porous membrane and the well plate in an orientation to position the at least one pore opposite of the at least one well, wherein the means for assembling are part of the porous membrane and/or the well plate, iv. a cover for sealing the at least one well with the at least one pore localized opposite of the at least one well after assembling the porous membrane and the well plate, and - filtering a liquid cell suspension comprising cells of interest through the porous membrane, - assembling the porous membrane and the well plate in an orientation to position the at least one pore opposite of the at least one well, - sealing the at least one well and the at least one pore localized opposite of the well with the cover, - analysis of at least one cellular component of a retained single cell in the at least one sealed well. - provision of i. a porous membrane comprising at least one pore, wherein the pore is adjusted to trap a single cell of interest when the cell is applied to the membrane in a liquid medium and to allow smaller or more flexible cells of the cell suspension to pass through the pore, ii. a well plate comprising at least one well, wherein the well diameter is larger than the pore diameter and the at least one well contains reagents for analyzing at least one component of the cells of interest, wherein the reagents can be provided in liquid or dry form, iii. means for assembling the porous membrane and the well plate in an orientation to position the at least one pore opposite of the at least one well, wherein the means for assembling are part of the porous membrane and/or the well plate, iv. a cover for sealing the at least one well with the at least one pore localized opposite of the at least one well after assembling the porous membrane and the well plate, and i. a porous membrane comprising at least one pore, wherein the pore is adjusted to trap a single cell of interest when the cell is applied to the membrane in a liquid medium and to allow smaller or more flexible cells of the cell suspension to pass through the pore, ii. a well plate comprising at least one well, wherein the well diameter is larger than the pore diameter and the at least one well contains reagents for analyzing at least one component of the cells of interest, wherein the reagents can be provided in liquid or dry form, iii. means for assembling the porous membrane and the well plate in an orientation to position the at least one pore opposite of the at least one well, wherein the means for assembling are part of the porous membrane and/or the well plate, iv. a cover for sealing the at least one well with the at least one pore localized opposite of the at least one well after assembling the porous membrane and the well plate, and - filtering a liquid cell suspension comprising cells of interest through the porous membrane, - assembling the porous membrane and the well plate in an orientation to position the at least one pore opposite of the at least one well, - sealing the at least one well and the at least one pore localized opposite of the well with the cover, - analysis of at least one cellular component of a retained single cell in the at least one sealed well.