Method for the detection and/or enrichment of a large number of different analyte proteins and/or analyte peptides from a sample mixture which includes proteins and/or peptides, whereby the method includes the following steps: a) provision of the sample mixture and fragmentation of the proteins contained therein into defined peptides by tryptic digestion, b) provision of first binding molecules which are specific for a peptide epitope of a large number of the various analyte proteins and/or analyte peptides, whereby the peptide epitope includes three, four or five amino acids, c) incubation of the first binding molecules with the sample mixture, and d) detection and/or enrichment of the analyte proteins and/or analyte peptides bound to the first binding molecules, by using second binding molecules which are specific for a peptide epitope of a large number of the various analyte proteins and/or analyte peptides, whereby the peptide epitope includes three, four or five amino acids, and specifically recognize analyte proteins and/or analyte peptides which are bound to the first binding molecules, wherein the first binding molecules are specific for one of the two terminal peptide epitopes of the analyte peptides, whereby the terminal peptide epitope includes the free NH 2 group or the free COOH group and three to five amino acids, and the second binding molecules are specific for the other terminal peptide epitopes of the analyte peptides, whereby the terminal peptide epitope includes the free COOH group or the free NH 2 group and three to five amino acids, such that an analyte peptide is detected by combined binding of first and second binding molecules to both free terminal epitopes of the analyte peptide. a) provision of the sample mixture and fragmentation of the proteins contained therein into defined peptides by tryptic digestion, b) provision of first binding molecules which are specific for a peptide epitope of a large number of the various analyte proteins and/or analyte peptides, whereby the peptide epitope includes three, four or five amino acids, c) incubation of the first binding molecules with the sample mixture, and d) detection and/or enrichment of the analyte proteins and/or analyte peptides bound to the first binding molecules, by using second binding molecules which are specific for a peptide epitope of a large number of the various analyte proteins and/or analyte peptides, whereby the peptide epitope includes three, four or five amino acids, and specifically recognize analyte proteins and/or analyte peptides which are bound to the first binding molecules, wherein the first binding molecules are specific for one of the two terminal peptide epitopes of the analyte peptides, whereby the terminal peptide epitope includes the free NH 2 group or the free COOH group and three to five amino acids, and the second binding molecules are specific for the other terminal peptide epitopes of the analyte peptides, whereby the terminal peptide epitope includes the free COOH group or the free NH 2 group and three to five amino acids, such that an analyte peptide is detected by combined binding of first and second binding molecules to both free terminal epitopes of the analyte peptide.