A disinfection system for reusable medical instruments, comprising: - a disinfection chamber, the disinfection chamber having: • an interior volume; • at least one radiation source, which, when enabled, output an UV disinfecting radiation, the at least one radiation source being suitable for emitting sufficient UV disinfecting radiation to carry out high-level disinfection; • a disinfection chamber controller configured to perform an high-level disinfection process via enablement of the at least one radiation source; and - a disinfection tray device suitable to be placed into the disinfection chamber, the disinfection tray device comprising at least one UV-transparent receptacle for receiving at least a part of at least an instrument to be disinfected, the at least one UV-transparent receptacle being arranged for a specific orientation and placement of an instrument to be disinfected, wherein the disinfection chamber controller is configured to enable the at least one radiation source so as to emit a dose of radiation comprised between 10 Millijoules per square centimeter and 10 Joules per square centimeter for a duration comprised between 10 seconds and 10 minutes, wherein the disinfection tray device comprises at least one first registration feature, the at least one first registration feature enabling the alignment of the instrument to be disinfected into the at least one UV-transparent receptacle. wherein the at least one first registration feature is a non-electronic, physical structure, the at least one first registration feature being a template printed on the surface of the UV-transparent receptacle. - a disinfection chamber, the disinfection chamber having: • an interior volume; • at least one radiation source, which, when enabled, output an UV disinfecting radiation, the at least one radiation source being suitable for emitting sufficient UV disinfecting radiation to carry out high-level disinfection; • a disinfection chamber controller configured to perform an high-level disinfection process via enablement of the at least one radiation source; and • an interior volume; • at least one radiation source, which, when enabled, output an UV disinfecting radiation, the at least one radiation source being suitable for emitting sufficient UV disinfecting radiation to carry out high-level disinfection; • a disinfection chamber controller configured to perform an high-level disinfection process via enablement of the at least one radiation source; and - a disinfection tray device suitable to be placed into the disinfection chamber, the disinfection tray device comprising at least one UV-transparent receptacle for receiving at least a part of at least an instrument to be disinfected, the at least one UV-transparent receptacle being arranged for a specific orientation and placement of an instrument to be disinfected, wherein the disinfection chamber controller is configured to enable the at least one radiation source so as to emit a dose of radiation comprised between 10 Millijoules per square centimeter and 10 Joules per square centimeter for a duration comprised between 10 seconds and 10 minutes, wherein the disinfection tray device comprises at least one first registration feature, the at least one first registration feature enabling the alignment of the instrument to be disinfected into the at least one UV-transparent receptacle. wherein the at least one first registration feature is a non-electronic, physical structure, the at least one first registration feature being a template printed on the surface of the UV-transparent receptacle.