A light sheet microscope (100, 500), comprising: an object slide configured to hold an object on a slide plane, an optical illumination system (108, 508) including an illumination objective configured to illuminate a first plane of the object with a light sheet, said first object plane being oblique relative to the slide plane, and an optical detection system including a detection objective and an image sensor device configured to define a first image plane which is orthogonal to an optical axis of the detection objective, wherein the detection objective is configured to image a focal plane onto the first image plane, said focal plane being coincident with the first object plane illuminated with the light sheet, wherein the image sensor device is further configured to define a second image plane (124, 224) which is tilted relative to the first image plane, and the detection objective is configured to image a second plane of the object onto the second image plane (124, 224), said second object plane being parallel to or coincident with the slide plane, and wherein the image sensor device comprises a first image sensor defining the first image plane and a second image sensor defining the second image plane, or wherein the image sensor device comprises one single image sensor which is tiltable between a first position in which a light receiving layer of the single image sensor is oriented to define the first image plane, and a second position, in which the light receiving layer is oriented to define the second image plane, characterized in that the optical detection system comprises an aperture configured to define a light bundle for imaging the second object plane onto the second image plane; and the aperture is located eccentrically with respect to the optical axis of the detection objective. an object slide configured to hold an object on a slide plane, an optical illumination system (108, 508) including an illumination objective configured to illuminate a first plane of the object with a light sheet, said first object plane being oblique relative to the slide plane, and an optical detection system including a detection objective and an image sensor device configured to define a first image plane which is orthogonal to an optical axis of the detection objective, wherein the detection objective is configured to image a focal plane onto the first image plane, said focal plane being coincident with the first object plane illuminated with the light sheet, wherein the image sensor device is further configured to define a second image plane (124, 224) which is tilted relative to the first image plane, and the detection objective is configured to image a second plane of the object onto the second image plane (124, 224), said second object plane being parallel to or coincident with the slide plane, and wherein the image sensor device comprises a first image sensor defining the first image plane and a second image sensor defining the second image plane, or wherein the image sensor device comprises one single image sensor which is tiltable between a first position in which a light receiving layer of the single image sensor is oriented to define the first image plane, and a second position, in which the light receiving layer is oriented to define the second image plane, characterized in that the optical detection system comprises an aperture configured to define a light bundle for imaging the second object plane onto the second image plane; and the aperture is located eccentrically with respect to the optical axis of the detection objective.