A method for screening a prey which interacts with a bait, the method including (a) preparing a cell which expresses (i) a first fusion construct comprising a translocation module, a first labeling material, and a first medium, and (ii) a third fusion construct comprising a prey and a second labeling material; (b) introducing (iii) a second fusion construct into the prepared cell, the second construct comprising a bait and a second medium binding with the first medium; (c) allowing the prey and the bait to interact each other; (d) inducing a translocation of the translocation module via an intrinsic signaling mechanism or by treating with a signaling material that generates an external signal; and (e) confirming the interaction between the prey and the bait by detecting intracellular distributions of the first construct and the third construct, wherein the bait of the second fusion construct and the prey of the third fusion construct are independently selected from the group consisting of protein, polypeptide, polysaccharide, polynucleotide, small organic molecule, synthetic compound, chemical compound and a combination thereof, wherein the first labeling material and the second labeling material are a material capable of generating a signal that can be distinctively detected, and wherein the first labeling material and the second labeling material are independently selected from the group consisting of a fluorescent material, a ligand, a light-emitting material, a microparticle, a redox material and a radioactive isotope, wherein the translocation module is selected from the group consisting of protein kinase C represented by an amino acid sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, and SEQ ID NO: 7, wherein the signaling material is selected from the group consisting of phorbol-12-myristate 13-acetate (PMA; phorbol ester), 12-0-tetradecanoylphorbol-13-acetate, phorbol-12,13-dibutyrate (PDBu), adenosine triphosphate, tridecanoic acid, arachidonic acid, linoleic acid, DiC8, 130C937, protein kinase C activation-related growth factors, and other protein kinase C activating materials, wherein the first medium of the first fusion construct and the second medium of the second fusion construct have high binding specificity and strength therebetween, wherein the first medium is a material capable of being directly produced through gene translation and expression in the cell, and wherein the second medium is a material having a small molecular weight capable of easily penetrating into the cell. (a) preparing a cell which expresses (i) a first fusion construct comprising a translocation module, a first labeling material, and a first medium, and (ii) a third fusion construct comprising a prey and a second labeling material; (b) introducing (iii) a second fusion construct into the prepared cell, the second construct comprising a bait and a second medium binding with the first medium; (c) allowing the prey and the bait to interact each other; (d) inducing a translocation of the translocation module via an intrinsic signaling mechanism or by treating with a signaling material that generates an external signal; and (e) confirming the interaction between the prey and the bait by detecting intracellular distributions of the first construct and the third construct,