A method for expanding tumor infiltrating lymphocytes (TII,S) into a therapeutic population of TILs comprising: (a) adding processed tumor fragments comprising a first population of TILs from a tumor resected from a subject into a closed system; (b) performing a first expansion by culturing the first population of TILs in a cell culture medium comprising IL-2 to produce a second population of TILs, wherein the first expansion is performed in a closed container providing a first gas-permeable surface area, wherein the first expansion is performed for 3-11 days to obtain the second population of TILs, wherein the second population of TILs is at least 50-fold greater in number than the first population of TILs, and wherein the transition from step (a) to step (b) occurs without opening the system; (c) performing a second expansion by supplementing the cell culture medium of the second population of TILs with additional IL-2,OKT-3, and antigen presenting cells (APCs), to produce a third population of TILs, wherein the second expansion is performed for 7-11 days to obtain the third population of TILs, wherein the third population of TILs is a therapeutic population of TILs, wherein the second expansion is performed in a closed container providing a second gas-permeable surface area, and wherein the transition from step (b) to step (c) occurs without opening the system; (d) harvesting the third population of TILs obtained from step (c), wherein the transition from step (c) to step (d) occurs without opening the system; (e) transferring the harvested third TIL population from step (d) to an infusion bag, wherein the transfer from step (d) to (e) occurs without opening the system; and (f) cryopreserving the infusion bag comprising the harvested TIL population from step (e) using a cryopreservation process, wherein the therapeutic population of TILs harvested in step (d) comprises sufficient TILs for a therapeutically effective dosage of the TILs, wherein the method comprises assaying the TILs for viability and wherein the viability of the therapeutic population of TILs once thawed is greater than or equal to 70%. (a) adding processed tumor fragments comprising a first population of TILs from a tumor resected from a subject into a closed system; (b) performing a first expansion by culturing the first population of TILs in a cell culture medium comprising IL-2 to produce a second population of TILs, wherein the first expansion is performed in a closed container providing a first gas-permeable surface area, wherein the first expansion is performed for 3-11 days to obtain the second population of TILs, wherein the second population of TILs is at least 50-fold greater in number than the first population of TILs, and wherein the transition from step (a) to step (b) occurs without opening the system; (c) performing a second expansion by supplementing the cell culture medium of the second population of TILs with additional IL-2,OKT-3, and antigen presenting cells (APCs), to produce a third population of TILs, wherein the second expansion is performed for 7-11 days to obtain the third population of TILs, wherein the third population of TILs is a therapeutic population of TILs, wherein the second expansion is performed in a closed container providing a second gas-permeable surface area, and wherein the transition from step (b) to step (c) occurs without opening the system; (d) harvesting the third population of TILs obtained from step (c), wherein the transition from step (c) to step (d) occurs without opening the system; (e) transferring the harvested third TIL population from step (d) to an infusion bag, wherein the transfer from step (d) to (e) occurs without opening the system; and (f) cryopreserving the infusion bag comprising the harvested TIL population from step (e) using a cryopreservation process, wherein the therapeutic population of TILs harvested in step (d) comprises sufficient TILs for a therapeutically effective dosage of the TILs, wherein the method comprises assaying the TILs for viability and wherein the viability of the therapeutic population of TILs once thawed is greater than or equal to 70%.