A method for detecting human hepatitis B virus in a sample suspected of containing HBV, which method comprises: (a) contacting a sample obtained from a human with a set of oligonucleotide sequences and reagents for amplification and detection of nucleic acid sequences, wherein the set of oligonucleotide sequences comprises (i) a forward primer oligonucleotide sequence comprising SEQ ID NO: 1; (ii) a reverse primer oligonucleotide sequence comprising SEQ ID NO: 2; (iii) a first probe oligonucleotide sequence comprising SEQ ID NO: 3; and (iv) a second probe oligonucleotide sequence comprising SEQ ID NO: 4, wherein each of the first and second probe oligonucleotide sequences comprises a detectable label, (b) amplifying a first target HBV nucleic acid sequence present in the sample, (c) hybridizing the first and second oligonucleotide probes to the first target HBV nucleic acid sequence, and (d) detecting hybridization of the first and second probe oligonucleotide sequences to the first target HBV nucleic acid sequence by assessing a signal from each of the detectable labels, whereby (i) the presence of the signals indicates hybridization of the first and second probe oligonucleotide sequences to the first target HBV nucleic acid sequence and the presence of HBV in the sample, and (ii) the absence of the signals indicates the absence of HBV in the sample, and wherein steps (a)-(d) are performed in approximately two hours starting from the sample. (a) contacting a sample obtained from a human with a set of oligonucleotide sequences and reagents for amplification and detection of nucleic acid sequences, wherein the set of oligonucleotide sequences comprises (i) a forward primer oligonucleotide sequence comprising SEQ ID NO: 1; (ii) a reverse primer oligonucleotide sequence comprising SEQ ID NO: 2; (iii) a first probe oligonucleotide sequence comprising SEQ ID NO: 3; and (iv) a second probe oligonucleotide sequence comprising SEQ ID NO: 4, wherein each of the first and second probe oligonucleotide sequences comprises a detectable label, (i) a forward primer oligonucleotide sequence comprising SEQ ID NO: 1; (ii) a reverse primer oligonucleotide sequence comprising SEQ ID NO: 2; (iii) a first probe oligonucleotide sequence comprising SEQ ID NO: 3; and (iv) a second probe oligonucleotide sequence comprising SEQ ID NO: 4, (b) amplifying a first target HBV nucleic acid sequence present in the sample, (c) hybridizing the first and second oligonucleotide probes to the first target HBV nucleic acid sequence, and (d) detecting hybridization of the first and second probe oligonucleotide sequences to the first target HBV nucleic acid sequence by assessing a signal from each of the detectable labels, whereby (i) the presence of the signals indicates hybridization of the first and second probe oligonucleotide sequences to the first target HBV nucleic acid sequence and the presence of HBV in the sample, and (ii) the absence of the signals indicates the absence of HBV in the sample, and wherein steps (a)-(d) are performed in approximately two hours starting from the sample. (i) the presence of the signals indicates hybridization of the first and second probe oligonucleotide sequences to the first target HBV nucleic acid sequence and the presence of HBV in the sample, and (ii) the absence of the signals indicates the absence of HBV in the sample,