A fundus (Er) imaging device comprising: an imaging optical system that includes light projection and reception separating means that forms a light projection region (P1, P2), through which illumination light passes onto a pupil (Ep) of a subject eye, and a light receiving region, from which fundus (Er) reflection light of the illumination light is extracted on the pupil (Ep) of the subject eye, at different positions, and a light receiving element that receives the fundus (Er) reflection light extracted from the light receiving region; and a drive unit that adjusts a positional relationship between the subject eye and the imaging optical system, wherein the fundus (Er) imaging device obtains a fundus (Er) image based on a signal from the light receiving element, and the fundus (Er) imaging device further comprises imaging control means that enables to selectively set, as an alignment reference position being a reference for alignment between the subject eye and the imaging optical system, any one of a first reference position which is assumed that all the light projection region (P1, P2) and the light receiving region are formed inside a pupil (Ep) region of the subject eye, and a second reference position which is different from the first reference position and is assumed that a part of any one of the light projection region (P 1, P2) and the light receiving region is formed outside the pupil (Ep) region of the subject eye, and that induces alignment based on an alignment deviation from the set alignment reference position, wherein the light projection and reception separating means forms two light projection regions (P 1, P2) having different positions on a pupil (Ep) of the subject eye, the imaging control means sets a position, as the second reference position, at which one of the two light projection regions (P 1, P2) together with the light receiving region are disposed inside a pupil (Ep) of the subject eye more preferentially than a remaining one light projection region (P1, P2), characterized in that the imaging control means causes the illumination light to be projected to a fundus (Er) from both of the two light projection regions (P 1, P2) when the fundus (Er) image is captured in a case of inducing alignment to the first reference position, and causes the illumination light to be projected to the fundus (Er) from only one of the two light projection regions (P1, P2), which is preferentially disposed inside the pupil (Ep), when the fundus (Er) image is captured in a case of inducing alignment to the second reference position. an imaging optical system that includes light projection and reception separating means that forms a light projection region (P1, P2), through which illumination light passes onto a pupil (Ep) of a subject eye, and a light receiving region, from which fundus (Er) reflection light of the illumination light is extracted on the pupil (Ep) of the subject eye, at different positions, and a light receiving element that receives the fundus (Er) reflection light extracted from the light receiving region; and a drive unit that adjusts a positional relationship between the subject eye and the imaging optical system, wherein the fundus (Er) imaging device obtains a fundus (Er) image based on a signal from the light receiving element, and the fundus (Er) imaging device further comprises imaging control means that enables to selectively set, as an alignment reference position being a reference for alignment between the subject eye and the imaging optical system, any one of a first reference position which is assumed that all the light projection region (P1, P2) and the light receiving region are formed inside a pupil (Ep) region of the subject eye, and a second reference position which is different from the first reference position and is assumed that a part of any one of the light projection region (P 1, P2) and the light receiving region is formed outside the pupil (Ep) region of the subject eye, and that induces alignment based on an alignment deviation from the set alignment reference position, wherein the light projection and reception separating means forms two light projection regions (P 1, P2) having different positions on a pupil (Ep) of the subject eye, the imaging control means sets a position, as the second reference position, at which one of the two light projection regions (P 1, P2) together with the light receiving region are disposed inside a pupil (Ep) of the subject eye more preferentially than a remaining one light projection region (P1, P2), characterized in that the imaging control means causes the illumination light to be projected to a fundus (Er) from both of the two light projection regions (P 1, P2) when the fundus (Er) image is captured in a case of inducing alignment to the first reference position, and causes the illumination light to be projected to the fundus (Er) from only one of the two light projection regions (P1, P2), which is preferentially disposed inside the pupil (Ep), when the fundus (Er) image is captured in a case of inducing alignment to the second reference position.