Arrangement for light sheet microscopy, comprising - an illumination optical unit with an illumination objective for illuminating a sample, which is situated on a sample carrier in a medium, with a light sheet via an illumination beam path, wherein the optical axis of the illumination objective and the light sheet are located in a plane which includes an illumination angle (β) that differs from zero with the normal of a plane reference surface, in respect of which the sample carrier is aligned, - a detection optical unit comprising a detection objective in a detection beam path, the optical axis of which includes a detection angle (δ) that differs from zero with the normal of the reference surface, - a separation layer system comprising at least one layer made of a specified material with a specified thickness, which separates the medium from the illumination objective and the detection objective, wherein the separation layer system is in contact with the medium, at least in the region accessible to the illumination objective and the detection objective for illumination and detection purposes, respectively, with a base aligned parallel to the reference surface, characterized in that - the detection objective comprises a first adaptive optical detection correction element arranged in or introducible into the beam path and/or - the illumination objective comprises a first adaptive optical illumination correction element arranged in or introducible into the beam path, - by means of which aberrations, which arise on account of the oblique passage through interfaces of the separation layer system of light to be detected or of light for illuminating the sample, are reducible for a specified range of detection angles (δ) or illumination angles (β) and/or for a specified range of the thickness of the at least one layer of the layer separation system. - an illumination optical unit with an illumination objective for illuminating a sample, which is situated on a sample carrier in a medium, with a light sheet via an illumination beam path, wherein the optical axis of the illumination objective and the light sheet are located in a plane which includes an illumination angle (β) that differs from zero with the normal of a plane reference surface, in respect of which the sample carrier is aligned, - a detection optical unit comprising a detection objective in a detection beam path, the optical axis of which includes a detection angle (δ) that differs from zero with the normal of the reference surface, - a separation layer system comprising at least one layer made of a specified material with a specified thickness, which separates the medium from the illumination objective and the detection objective, wherein the separation layer system is in contact with the medium, at least in the region accessible to the illumination objective and the detection objective for illumination and detection purposes, respectively, with a base aligned parallel to the reference surface, characterized in that - the detection objective comprises a first adaptive optical detection correction element arranged in or introducible into the beam path and/or - the illumination objective comprises a first adaptive optical illumination correction element arranged in or introducible into the beam path, - by means of which aberrations, which arise on account of the oblique passage through interfaces of the separation layer system of light to be detected or of light for illuminating the sample, are reducible for a specified range of detection angles (δ) or illumination angles (β) and/or for a specified range of the thickness of the at least one layer of the layer separation system.