A particle capture device comprising: a first substrate being formed of a layer in which recessed portions are patterned and a layer in which connection holes are patterned, and having a structure in which a plurality of the recessed portions are vertically and horizontally disposed at equal intervals; and a second substrate that is disposed parallel to and facing a first side of the first substrate, wherein the plurality of recessed portions are open on the second side of the first substrate and have a size capable of capturing one particle, the connection holes connect the first side to the second side and have a size allowing a dispersion medium of the particles to move therethrough, a flow path that has the connection holes of the first substrate as an inlet port of the dispersion medium and has an end portion of the first side of the first substrate as an outlet port of the dispersion medium is formed between the first substrate and the second substrate, a total opening area of all the connection holes included in the particle device is 1 mm 2 or more and less than 10 mm 2, and a cross-sectional area of the flow path at the outlet port is 0.8 times or more the total opening area of the connection holes, or a total opening area of all the connection holes included in the particle device is 10 mm 2 or more and 1000 mm 2 or less, and a cross-sectional area of the flow path at the outlet port is 0.1 times or more the total opening area of the connection holes, a diameter of the particle is 1 to 500 μm, an opening area of the connection hole is the smallest cross-sectional area among cross-sectional areas of a surface, which is parallel to the first substrate, at any position of the connection holes that connect the first side to the second side. a first substrate being formed of a layer in which recessed portions are patterned and a layer in which connection holes are patterned, and having a structure in which a plurality of the recessed portions are vertically and horizontally disposed at equal intervals; and a second substrate that is disposed parallel to and facing a first side of the first substrate, wherein the plurality of recessed portions are open on the second side of the first substrate and have a size capable of capturing one particle, the connection holes connect the first side to the second side and have a size allowing a dispersion medium of the particles to move therethrough, a flow path that has the connection holes of the first substrate as an inlet port of the dispersion medium and has an end portion of the first side of the first substrate as an outlet port of the dispersion medium is formed between the first substrate and the second substrate, a total opening area of all the connection holes included in the particle device is 1 mm 2 or more and less than 10 mm 2, and a cross-sectional area of the flow path at the outlet port is 0.8 times or more the total opening area of the connection holes, or a total opening area of all the connection holes included in the particle device is 10 mm 2 or more and 1000 mm 2 or less, and a cross-sectional area of the flow path at the outlet port is 0.1 times or more the total opening area of the connection holes, a diameter of the particle is 1 to 500 μm, an opening area of the connection hole is the smallest cross-sectional area among cross-sectional areas of a surface, which is parallel to the first substrate, at any position of the connection holes that connect the first side to the second side.