An evaluation method for an influence of a substance to be tested on permeability of a substance through a central nervous system tissue, using an evaluation probe for central nervous system permeability which comprises a probe for visualizing a function of a transporter associated with at least one of influx and efflux in a central nervous system, wherein the probe comprises at least one kind of compound represented by the following general formula, General formula: where in the general formula: R 1 represents one of a hydrogen atom, an alkyl group, an aralkyl group, an alkenyl group, a substituted alkenyl group, a cycloalkenyl group, a cycloalkyl group, an aryl group, a substituted aryl group, a heterocyclic group, and an alkanoyl group; R 2 to R 5 each independently represent one of a hydrogen atom, an alkyl group, an aryl group, a carboxylic acid group, an alkoxycarbonyl group, and an alkanoyl group, provided that R 2 and R 4 may be bonded to each other to form a ring; R 6 represents one of a hydrogen atom and a halogen atom; R 7 represents one of an alkoxycarbonyl group, a carboxylic acid and a salt thereof; R 8 represents one of a sulfur atom or a ring represented by the following general formula; n represents an integer of 0 to 3; and m represents an integer of 1 or more, General formula where in the general formula: R 9 represents one of an alkyl group, an aralkyl group, an alkenyl group, and an aryl group; and "*" represents a bonding site; the method comprising a first step of administering, to a biological specimen, the evaluation probe as a first case; independently of the first step, a second step of administering, to a biological specimen, the evaluation probe and a substance to be tested as a second case, characterized by further comprising detecting, for the first and the second case, permeability of the probes through the central nervous system tissue by irradiating the biological specimens with excitation light and observing a fluorescence intensity derived from the probes in the central nervous system tissue multiple times with time; and evaluating the influence of the substance to be tested based on a variation of permeability through the central nervous system tissue in the second case compared to the first case. R 1 represents one of a hydrogen atom, an alkyl group, an aralkyl group, an alkenyl group, a substituted alkenyl group, a cycloalkenyl group, a cycloalkyl group, an aryl group, a substituted aryl group, a heterocyclic group, and an alkanoyl group; R 2 to R 5 each independently represent one of a hydrogen atom, an alkyl group, an aryl group, a carboxylic acid group, an alkoxycarbonyl group, and an alkanoyl group, provided that R 2 and R 4 may be bonded to each other to form a ring; R 6 represents one of a hydrogen atom and a halogen atom; R 7 represents one of an alkoxycarbonyl group, a carboxylic acid and a salt thereof; R 8 represents one of a sulfur atom or a ring represented by the following general formula; n represents an integer of 0 to 3; and m represents an integer of 1 or more, R 9 represents one of an alkyl group, an aralkyl group, an alkenyl group, and an aryl group; and "*" represents a bonding site; a first step of administering, to a biological specimen, the evaluation probe as a first case; independently of the first step, a second step of administering, to a biological specimen, the evaluation probe and a substance to be tested as a second case, characterized by further comprising detecting, for the first and the second case, permeability of the probes through the central nervous system tissue by irradiating the biological specimens with excitation light and observing a fluorescence intensity derived from the probes in the central nervous system tissue multiple times with time; and evaluating the influence of the substance to be tested based on a variation of permeability through the central nervous system tissue in the second case compared to the first case.