Use of a modified polynucleotide encoding a modified full-length protease, said modified polynucleotide comprising: a first polynucleotide encoding a signal peptide, said first polynucleotide being operably linked to a second polynucleotide encoding the pro region set forth in SEQ ID NO:7, wherein said pro region comprises a combination of substitutions of at least two amino acids at positions chosen from positions 6, 30 and 32 of said pro region, wherein said combination of substitutions is chosen from E6R-A32K, E6N-A32K,E6D-A32K, E6I-A32K, E6K-A32K, E6M-A32K, E6P-A32K, E6T-A32K, E6N-A32K, E6A-E30G, E6R-E30G, E6C-E30G, E6Q-E30G, E6G-E30G, E6H-E30G, E6K-E30G, E6S-E30G, E6W-E30G, E30G-A32R, E30G-A32Q, E30G-A32E, E30G-A32G, E30G-A32H, E30G-A32I, E30G-A32S, E30G-A32T, E30G-A32W, E30G-A32V, E6G-E30G-A32E, E6G-E30G-A32S, E6G-E30G-A32T, E6G-E30G-A32W, E6A-E30G, E6R-E30G, E6N-E30G, E6D-E30G, E6C-E30G, E6Q-E30G, E6G-E30G, E6H-E30G, E6K-E30G, E6M-E30G, E6F-E30G, E6P-E30G, E6S-E30G, E6T-E30G, E6W-E30G, E6V-E30G, E6Y-E30G, E6A-E30S, E6G-E30S, E6L-E30S, E6K-E30S, E6F-E30S, E6P-E30S, E6Y-E30S, E6V-E30S, E30S-A32R, E30S-A32N, E30S-A32D, E30S-A32C, E30S-A32Q, E30S-A32E, E30S-A32G, E30S-A32H, E30S-A32L, E30S-A32M, E30S-A32F, E30S-A32P, E30S-A32S, E30S-A32T, E30S-A32W, E30S-A32Y, and E30S-A32V and wherein the positions are numbered by correspondence with the amino acid sequence of the pro polypeptide of SEQ ID NO:7; said second polynucleotide being operably linked to a third polynucleotide encoding the mature region of a protease that is at least about 60% identical to the mature protease of SEQ ID NO: 9, to provide enhanced production of a mature protease that is processed from the modified full-length protease compared to the level of production of the same mature protease when processed from the full-length protease, without the substitutions in the pro region set forth in SEQ ID NO: 7. a first polynucleotide encoding a signal peptide, said first polynucleotide being operably linked to a second polynucleotide encoding the pro region set forth in SEQ ID NO:7, wherein said pro region comprises a combination of substitutions of at least two amino acids at positions chosen from positions 6, 30 and 32 of said pro region, wherein said combination of substitutions is chosen from E6R-A32K, E6N-A32K,E6D-A32K, E6I-A32K, E6K-A32K, E6M-A32K, E6P-A32K, E6T-A32K, E6N-A32K, E6A-E30G, E6R-E30G, E6C-E30G, E6Q-E30G, E6G-E30G, E6H-E30G, E6K-E30G, E6S-E30G, E6W-E30G, E30G-A32R, E30G-A32Q, E30G-A32E, E30G-A32G, E30G-A32H, E30G-A32I, E30G-A32S, E30G-A32T, E30G-A32W, E30G-A32V, E6G-E30G-A32E, E6G-E30G-A32S, E6G-E30G-A32T, E6G-E30G-A32W, E6A-E30G, E6R-E30G, E6N-E30G, E6D-E30G, E6C-E30G, E6Q-E30G, E6G-E30G, E6H-E30G, E6K-E30G, E6M-E30G, E6F-E30G, E6P-E30G, E6S-E30G, E6T-E30G, E6W-E30G, E6V-E30G, E6Y-E30G, E6A-E30S, E6G-E30S, E6L-E30S, E6K-E30S, E6F-E30S, E6P-E30S, E6Y-E30S, E6V-E30S, E30S-A32R, E30S-A32N, E30S-A32D, E30S-A32C, E30S-A32Q, E30S-A32E, E30S-A32G, E30S-A32H, E30S-A32L, E30S-A32M, E30S-A32F, E30S-A32P, E30S-A32S, E30S-A32T, E30S-A32W, E30S-A32Y, and E30S-A32V and wherein the positions are numbered by correspondence with the amino acid sequence of the pro polypeptide of SEQ ID NO:7; said second polynucleotide being operably linked to a third polynucleotide encoding the mature region of a protease that is at least about 60% identical to the mature protease of SEQ ID NO: 9, to provide enhanced production of a mature protease that is processed from the modified full-length protease compared to the level of production of the same mature protease when processed from the full-length protease, without the substitutions in the pro region set forth in SEQ ID NO: 7.