A method of performing a sample assay with a microassay cartridge said cartridge comprising a) a first molded housing having a pneumatic circuit enclosed therein; b) a second molded housing having a hydraulic circuit enclosed therein; c) a sample inlet for receiving a test sample, wherein said sample inlet is in fluid communication with said hydraulic circuit; d) a laminate layer interposed between said first molded housing and said second molded housing, said laminate layer (1301, 1302, 1303) comprising a plurality of pneumohydraulic membranes (1301, 1302, 1303) in fluid communication with said pneumatic circuit and said hydraulic circuit; e) an assay well assembly in fluid communication with said hydraulic circuit; f) an array of pneumatic ports defining a pneumatic interface, each port for receiving a pneumatic pulse applied thereto, said ports are in fluid communication with said pneumatic circuit, wherein said pneumatic pulse is a positive pressure pulse or a negative pressure pulse; and wherein said cartridge is enabled such that said pneumohydraulic membranes (1301, 1302, 1303) are operably controlled by said pneumatic pulses, wherein said hydraulic circuit comprises a test assay circuit and a control assay circuit,wherein said test assay circuit is in fluid communication with said sample inlet and said control assay circuit not in fluid communication with said sample inlet, wherein said control assay circuit comprises a positive control assay circuit and a negative control assay circuit, wherein the method is characterized in that for each unique target assay run the corresponding test assay and a positive and negative control assay are executed in parallel in the respective test assay circuit, positive control assay circuit and a negative control assay circuit. a) a first molded housing having a pneumatic circuit enclosed therein; b) a second molded housing having a hydraulic circuit enclosed therein; c) a sample inlet for receiving a test sample, wherein said sample inlet is in fluid communication with said hydraulic circuit; d) a laminate layer interposed between said first molded housing and said second molded housing, said laminate layer (1301, 1302, 1303) comprising a plurality of pneumohydraulic membranes (1301, 1302, 1303) in fluid communication with said pneumatic circuit and said hydraulic circuit; e) an assay well assembly in fluid communication with said hydraulic circuit; f) an array of pneumatic ports defining a pneumatic interface, each port for receiving a pneumatic pulse applied thereto, said ports are in fluid communication with said pneumatic circuit, wherein said pneumatic pulse is a positive pressure pulse or a negative pressure pulse; and wherein said cartridge is enabled such that said pneumohydraulic membranes (1301, 1302, 1303) are operably controlled by said pneumatic pulses, wherein said hydraulic circuit comprises a test assay circuit and a control assay circuit,wherein said test assay circuit is in fluid communication with said sample inlet and said control assay circuit not in fluid communication with said sample inlet, wherein said control assay circuit comprises a positive control assay circuit and a negative control assay circuit, wherein the method is characterized in that for each unique target assay run the corresponding test assay and a positive and negative control assay are executed in parallel in the respective test assay circuit, positive control assay circuit and a negative control assay circuit. wherein said cartridge is enabled such that said pneumohydraulic membranes (1301, 1302, 1303) are operably controlled by said pneumatic pulses, wherein said hydraulic circuit comprises a test assay circuit and a control assay circuit,wherein said test assay circuit is in fluid communication with said sample inlet and said control assay circuit not in fluid communication with said sample inlet, wherein said control assay circuit comprises a positive control assay circuit and a negative control assay circuit, wherein the method is characterized in that for each unique target assay run the corresponding test assay and a positive and negative control assay are executed in parallel in the respective test assay circuit, positive control assay circuit and a negative control assay circuit.