A method of detecting an analyte in a test sample, the method comprising; i) providing a lateral flow assay device that comprises a chromatographic medium that includes: (a) a sample loading zone located upstream of a detection zone; (b) a reporting carrier zone located between the sample loading zone and a detection zone, wherein said reporting carrier zone comprises a reporting carrier, said reporting carrier comprising a carrier and one or more proficient enzyme cassettes, wherein the carrier is capable of forming a complex with the analyte, wherein the proficient enzyme cassette contains at least one proficient enzyme that is conjugated to the reporting carrier, wherein the proficient enzyme is an enzyme having a turnover constant of 10 3 per second per enzyme or higher; and (c) a detection zone, wherein the detection zone comprises a capture component and an indicator, wherein the capture component is configured to specifically recognize and complex with the analyte without preventing a reporting carrier from forming a complex with the analyte; ii) contacting the sample loading zone with the test sample, wherein the test sample travels through the reporting carrier zone along the chromatographic medium from the sample loading zone to the detection zone and beyond the detection zone; iii) adding a substrate to the detection zone wherein the substrate undergoes a reaction in the presence of proficient enzyme analyte containing reporting carrier; and iv) generating a response of the indicator within the detection zone that corresponds to the presence or absence of the analyte in the test sample. i) providing a lateral flow assay device that comprises a chromatographic medium that includes: (a) a sample loading zone located upstream of a detection zone; (b) a reporting carrier zone located between the sample loading zone and a detection zone, wherein said reporting carrier zone comprises a reporting carrier, said reporting carrier comprising a carrier and one or more proficient enzyme cassettes, wherein the carrier is capable of forming a complex with the analyte, wherein the proficient enzyme cassette contains at least one proficient enzyme that is conjugated to the reporting carrier, wherein the proficient enzyme is an enzyme having a turnover constant of 10 3 per second per enzyme or higher; and (c) a detection zone, wherein the detection zone comprises a capture component and an indicator, wherein the capture component is configured to specifically recognize and complex with the analyte without preventing a reporting carrier from forming a complex with the analyte; (a) a sample loading zone located upstream of a detection zone; (b) a reporting carrier zone located between the sample loading zone and a detection zone, wherein said reporting carrier zone comprises a reporting carrier, said reporting carrier comprising a carrier and one or more proficient enzyme cassettes, wherein the carrier is capable of forming a complex with the analyte, wherein the proficient enzyme cassette contains at least one proficient enzyme that is conjugated to the reporting carrier, wherein the proficient enzyme is an enzyme having a turnover constant of 10 3 per second per enzyme or higher; and (c) a detection zone, wherein the detection zone comprises a capture component and an indicator, wherein the capture component is configured to specifically recognize and complex with the analyte without preventing a reporting carrier from forming a complex with the analyte; ii) contacting the sample loading zone with the test sample, wherein the test sample travels through the reporting carrier zone along the chromatographic medium from the sample loading zone to the detection zone and beyond the detection zone; iii) adding a substrate to the detection zone wherein the substrate undergoes a reaction in the presence of proficient enzyme analyte containing reporting carrier; and iv) generating a response of the indicator within the detection zone that corresponds to the presence or absence of the analyte in the test sample.