A method for the multiplexed analysis of one or more intracellular targets of a single cell, comprising contacting a single cell of a biological sample with a composition comprising: a surface comprising a plurality of capture agents operatively-linked thereto, wherein each capture agent specifically binds to a distinct intracellular target and the plurality of capture agents form a repeating pattern; a substrate comprising a plurality of chambers, wherein the substrate releasably couples with the surface and each chamber of the plurality of chambers comprises at least one repeat of the repeating pattern of the plurality of capture agents of the surface; and a coating composition comprising a cell lysis composition; wherein the coating composition is coated on the substrate and/or surface; and a linker composition comprising a functionalization component and an extension component; wherein: contacting is performed under conditions sufficient to lyse the single cell to produce a single cell lysate within each chamber of the plurality of chambers of the substrate, and within each chamber of the plurality of chambers the single cell lysate is in fluid communication with the surface; incubating the single cell lysate within each chamber of the plurality of chambers for a sufficient time to allow at least one capture agent of the plurality of capture agents to specifically bind to at least one intracellular target to produce a capture agent:target complex; and visualizing at least one capture agent: target complex to identify one or more intracellular targets of a single cell in a multiplexed analysis. contacting a single cell of a biological sample with a composition comprising: a surface comprising a plurality of capture agents operatively-linked thereto, wherein each capture agent specifically binds to a distinct intracellular target and the plurality of capture agents form a repeating pattern; a substrate comprising a plurality of chambers, wherein the substrate releasably couples with the surface and each chamber of the plurality of chambers comprises at least one repeat of the repeating pattern of the plurality of capture agents of the surface; and a coating composition comprising a cell lysis composition; wherein the coating composition is coated on the substrate and/or surface; and a linker composition comprising a functionalization component and an extension component; wherein: contacting is performed under conditions sufficient to lyse the single cell to produce a single cell lysate within each chamber of the plurality of chambers of the substrate, and within each chamber of the plurality of chambers the single cell lysate is in fluid communication with the surface; a surface comprising a plurality of capture agents operatively-linked thereto, wherein each capture agent specifically binds to a distinct intracellular target and the plurality of capture agents form a repeating pattern; a substrate comprising a plurality of chambers, wherein the substrate releasably couples with the surface and each chamber of the plurality of chambers comprises at least one repeat of the repeating pattern of the plurality of capture agents of the surface; and a coating composition comprising a cell lysis composition; wherein the coating composition is coated on the substrate and/or surface; and a linker composition comprising a functionalization component and an extension component; contacting is performed under conditions sufficient to lyse the single cell to produce a single cell lysate within each chamber of the plurality of chambers of the substrate, and within each chamber of the plurality of chambers the single cell lysate is in fluid communication with the surface; incubating the single cell lysate within each chamber of the plurality of chambers for a sufficient time to allow at least one capture agent of the plurality of capture agents to specifically bind to at least one intracellular target to produce a capture agent:target complex; and visualizing at least one capture agent: target complex to identify one or more intracellular targets of a single cell in a multiplexed analysis.