A method for detecting an analyte concentration, comprising: providing a test strip comprising: a first electrode set comprising a first reaction area; a second electrode set comprising a second reaction area; and a reaction reagent layer disposed on the second reaction area; providing a blood sample and bringing the blood sample into contact with the first electrode set and the second electrode set; applying a first voltage to the first electrode set by a voltammetry to obtain a first response value, wherein the voltammetry is a square wave voltammetry; calculating a hematocrit value according to the first response value; applying a second voltage to the second electrode set to obtain a second response value; conducting a calibration to a linear equation related with the analyte concentration by using the hematocrit value to obtain a calibrated linear equation, wherein the method for conducting the calibration to the linear equation related with the analyte concentration by using the hematocrit value to obtain the calibrated linear equation comprises: obtaining a slope coefficient and an interception coefficient corresponding to the hematocrit value according to a reference table or a relationship equation; and conducting a calibration to the slope parameter and the interception parameter of the linear equation related with the analyte concentration by using the slope coefficient and the interception coefficient to obtain the calibrated linear equation; and calculating an actual value of the analyte concentration according to the second response value and the calibrated linear equation, wherein the linear equation related with the analyte concentration is y=ax+b, wherein y is the second response value, a is a slope parameter, x is the analyte concentration, b is an interception parameter, wherein the linear equation y=ax+b is a conversion equation of the second response value and the analyte concentration when the hematocrit value of the blood sample is 0 %, wherein the calibrated linear equation is y=a'x+b', wherein y is the second response value, a' is a calibrated slope parameter, x is the actual value of the analyte concentration, b' is a calibrated interception parameter. a first electrode set comprising a first reaction area; a second electrode set comprising a second reaction area; and a reaction reagent layer disposed on the second reaction area; providing a blood sample and bringing the blood sample into contact with the first electrode set and the second electrode set; applying a first voltage to the first electrode set by a voltammetry to obtain a first response value, wherein the voltammetry is a square wave voltammetry; calculating a hematocrit value according to the first response value; applying a second voltage to the second electrode set to obtain a second response value; conducting a calibration to a linear equation related with the analyte concentration by using the hematocrit value to obtain a calibrated linear equation, wherein the method for conducting the calibration to the linear equation related with the analyte concentration by using the hematocrit value to obtain the calibrated linear equation comprises: obtaining a slope coefficient and an interception coefficient corresponding to the hematocrit value according to a reference table or a relationship equation; and conducting a calibration to the slope parameter and the interception parameter of the linear equation related with the analyte concentration by using the slope coefficient and the interception coefficient to obtain the calibrated linear equation; and calculating an actual value of the analyte concentration according to the second response value and the calibrated linear equation, wherein the linear equation related with the analyte concentration is y=ax+b, wherein y is the second response value, a is a slope parameter, x is the analyte concentration, b is an interception parameter, wherein the linear equation y=ax+b is a conversion equation of the second response value and the analyte concentration when the hematocrit value of the blood sample is 0 %, wherein the calibrated linear equation is y=a'x+b', wherein y is the second response value, a' is a calibrated slope parameter, x is the actual value of the analyte concentration, b' is a calibrated interception parameter. obtaining a slope coefficient and an interception coefficient corresponding to the hematocrit value according to a reference table or a relationship equation; and conducting a calibration to the slope parameter and the interception parameter of the linear equation related with the analyte concentration by using the slope coefficient and the interception coefficient to obtain the calibrated linear equation; and calculating an actual value of the analyte concentration according to the second response value and the calibrated linear equation, wherein the linear equation related with the analyte concentration is y=ax+b, wherein y is the second response value, a is a slope parameter, x is the analyte concentration, b is an interception parameter, wherein the linear equation y=ax+b is a conversion equation of the second response value and the analyte concentration when the hematocrit value of the blood sample is 0 %, wherein the calibrated linear equation is y=a'x+b', wherein y is the second response value, a' is a calibrated slope parameter, x is the actual value of the analyte concentration, b' is a calibrated interception parameter.