A method for obtaining a high affinity T cell receptor specific for a peptide of interest, a recombinant cell expressing a TCR specific for a peptide of interest and/or a nucleic acid encoding a TCR polypeptide chain that can form a TCR specific for a peptide of interest and wherein the high affinity is higher than a preselected standard or is increased for the peptide of interest compared to the parent TCR comprising: a. transducing a cell population comprising cells able to express a TCR and/or differentiate into cells able to express a TCR with a bait nucleic acid encoding a bait human TCR polypeptide chain, wherein the bait human TCR polypeptide chain constitutes a parent TCR with a counterchain TCR polypeptide chain that specifically binds said peptide of interest; b. culturing the transduced cell population under conditions that permit the bait TCR polypeptide chain to be expressed; and c. i) obtaining a recombinant cell expressing a TCR comprising the bait TCR polypeptide chain and a prey TCR polypeptide chain that selectively binds said peptide of interest said obtaining step comprising testing the avidity of the recombinant cell expressing the TCR for peptide binding avidity and enriching for high avidity T cells and wherein the high avidity is higher than a preselected standard or is increased for the peptide of interest compared to the parent TCR and said enriching step comprising culturing the transduced cell population with an antigen presenting cell presenting the peptide of interest; ii) isolating a prey nucleic acid encoding the prey TCR polypeptide chain from the recombinant cell obtained in step (c)(i); and iii) pairing the prey nucleic acid isolated in step (c)(ii) with the bait nucleic acid, wherein the prey TCR chain paired with the bait TCR chain constitutes a TCR with higher affinity than a preselected affinity. a. transducing a cell population comprising cells able to express a TCR and/or differentiate into cells able to express a TCR with a bait nucleic acid encoding a bait human TCR polypeptide chain, wherein the bait human TCR polypeptide chain constitutes a parent TCR with a counterchain TCR polypeptide chain that specifically binds said peptide of interest; b. culturing the transduced cell population under conditions that permit the bait TCR polypeptide chain to be expressed; and c. i) obtaining a recombinant cell expressing a TCR comprising the bait TCR polypeptide chain and a prey TCR polypeptide chain that selectively binds said peptide of interest said obtaining step comprising testing the avidity of the recombinant cell expressing the TCR for peptide binding avidity and enriching for high avidity T cells and wherein the high avidity is higher than a preselected standard or is increased for the peptide of interest compared to the parent TCR and said enriching step comprising culturing the transduced cell population with an antigen presenting cell presenting the peptide of interest; ii) isolating a prey nucleic acid encoding the prey TCR polypeptide chain from the recombinant cell obtained in step (c)(i); and iii) pairing the prey nucleic acid isolated in step (c)(ii) with the bait nucleic acid, wherein the prey TCR chain paired with the bait TCR chain constitutes a TCR with higher affinity than a preselected affinity. i) obtaining a recombinant cell expressing a TCR comprising the bait TCR polypeptide chain and a prey TCR polypeptide chain that selectively binds said peptide of interest said obtaining step comprising testing the avidity of the recombinant cell expressing the TCR for peptide binding avidity and enriching for high avidity T cells and wherein the high avidity is higher than a preselected standard or is increased for the peptide of interest compared to the parent TCR and said enriching step comprising culturing the transduced cell population with an antigen presenting cell presenting the peptide of interest; ii) isolating a prey nucleic acid encoding the prey TCR polypeptide chain from the recombinant cell obtained in step (c)(i); and iii) pairing the prey nucleic acid isolated in step (c)(ii) with the bait nucleic acid, wherein the prey TCR chain paired with the bait TCR chain constitutes a TCR with higher affinity than a preselected affinity.