A method of analyzing a hematoxylin and eosin (H&E) stained biological sample, comprising: (a) optionally detecting the hematoxylin and eosin staining of the biological sample containing multiple targets; (b) removing fluorescent H&E signal from the hematoxylin and eosin stained biological sample; and (c) detecting additional features or targets in the biological sample; wherein the removing step comprises: (i) washing the sample using a buffered aqueous solution to remove the hematoxylin and partially remove the eosin; and, (ii) contacting the sample with a charge transfer reagent to quench the residual fluorescence wherein said charge transfer reagent is selected from Methyl Viologen (p-Quat), diquat or phenylene diamine dihydrochloride salts; or, (i) washing the sample using a buffered aqueous solution to remove the hematoxylin and partially remove the eosin; (ii) contacting the sample with hydrogen peroxide and a metal salt to generate hydroxyl radicals; and, (iii) irradiating the sample; or, (i) washing the sample using a buffered aqueous solution to remove the hematoxylin and partially remove the eosin; (ii) contacting the sample with an electron transfer reagent wherein the electron transfer reagent is a borate salt represented by the following structural formula: wherein: each R 1, R 2, and R 3 is phenyl and R 4 is butyl, or benzyl, and M + is selected from the group consisting of organic and inorganic cations; and, (iii) irradiating the sample to remove residual fluorescence. (a) optionally detecting the hematoxylin and eosin staining of the biological sample containing multiple targets; (b) removing fluorescent H&E signal from the hematoxylin and eosin stained biological sample; and (c) detecting additional features or targets in the biological sample; wherein the removing step comprises: (i) washing the sample using a buffered aqueous solution to remove the hematoxylin and partially remove the eosin; and, (ii) contacting the sample with a charge transfer reagent to quench the residual fluorescence wherein said charge transfer reagent is selected from Methyl Viologen (p-Quat), diquat or phenylene diamine dihydrochloride salts; or, (i) washing the sample using a buffered aqueous solution to remove the hematoxylin and partially remove the eosin; (ii) contacting the sample with hydrogen peroxide and a metal salt to generate hydroxyl radicals; and, (iii) irradiating the sample; or, (i) washing the sample using a buffered aqueous solution to remove the hematoxylin and partially remove the eosin; (ii) contacting the sample with an electron transfer reagent wherein the electron transfer reagent is a borate salt represented by the following structural formula: wherein: each R 1, R 2, and R 3 is phenyl and R 4 is butyl, or benzyl, and M + is selected from the group consisting of organic and inorganic cations; and, (iii) irradiating the sample to remove residual fluorescence. (i) washing the sample using a buffered aqueous solution to remove the hematoxylin and partially remove the eosin; and, (ii) contacting the sample with a charge transfer reagent to quench the residual fluorescence wherein said charge transfer reagent is selected from Methyl Viologen (p-Quat), diquat or phenylene diamine dihydrochloride salts; or, (i) washing the sample using a buffered aqueous solution to remove the hematoxylin and partially remove the eosin; (ii) contacting the sample with hydrogen peroxide and a metal salt to generate hydroxyl radicals; and, (iii) irradiating the sample; or, (i) washing the sample using a buffered aqueous solution to remove the hematoxylin and partially remove the eosin; (ii) contacting the sample with an electron transfer reagent wherein the electron transfer reagent is a borate salt represented by the following structural formula: wherein: each R 1, R 2, and R 3 is phenyl and R 4 is butyl, or benzyl, and M + is selected from the group consisting of organic and inorganic cations; and, (iii) irradiating the sample to remove residual fluorescence. (i) washing the sample using a buffered aqueous solution to remove the hematoxylin and partially remove the eosin; (ii) contacting the sample with hydrogen peroxide and a metal salt to generate hydroxyl radicals; and, (iii) irradiating the sample; or, (i) washing the sample using a buffered aqueous solution to remove the hematoxylin and partially remove the eosin; (ii) contacting the sample with an electron transfer reagent wherein the electron transfer reagent is a borate salt represented by the following structural formula: wherein: each R 1, R 2, and R 3 is phenyl and R 4 is butyl, or benzyl, and M + is selected from the group consisting of organic and inorganic cations; and, (iii) irradiating the sample to remove residual fluorescence. (i) washing the sample using a buffered aqueous solution to remove the hematoxylin and partially remove the eosin; (ii) contacting the sample with an electron transfer reagent wherein the electron transfer reagent is a borate salt represented by the following structural formula: wherein: each R 1, R 2, and R 3 is phenyl and R 4 is butyl, or benzyl, and M + is selected from the group consisting of organic and inorganic cations; and, (iii) irradiating the sample to remove residual fluorescence.