A method for preparing a pharmaceutical composition for relieving fatigue, comprising: mixing the following eight substances for obtaining a mixture, the mixture is the pharmaceutical composition for relieving the fatigue: A. ethanol extract of ginseng; B. ethanol extract of astragalus; C. ethanol extract of epimedium; D. ethanol extract of yam; E. ethanol extract of polygonatum; F. ethanol extract of dodder; G. aqueous extract of wolfberry; H. a fermentation conversion solution of fruits and vegetables; wherein a method for preparing the A substance comprises: processing reflux extraction on the ginseng with an aqueous solution of ethanol for obtaining the extract, wherein the extract is the ethanol extract of the ginseng; wherein a method for preparing the B substance comprises: processing the reflux extraction on the astragalus with the aqueous solution of the ethanol for obtaining the extract, wherein the extract is the ethanol extract of the astragalus; wherein a method for preparing the C substance comprises: processing the reflux extraction on the epimedium with the aqueous solution of the ethanol for obtaining the extract, wherein the extract is the ethanol extract of the epimedium; wherein a method for preparing the D substance comprises: processing the reflux extraction on the yam with the aqueous solution of the ethanol for obtaining the extract, wherein the extract is the ethanol extract of the yam; wherein a method for preparing the E substance comprises: processing the reflux extraction on the polygonatum with the aqueous solution of the ethanol for obtaining the extract, wherein the extract is the ethanol extract of the polygonatum; wherein a method for preparing the F substance comprises: processing the reflux extraction on the dodder with the aqueous solution of the ethanol for obtaining the extract, wherein the extract is the ethanol extract of the dodder; wherein a method for preparing the G substance comprises: processing heating extraction on the wolfberry with pure water for obtaining the extract, wherein the extract is the aqueous extract of the wolfberry; wherein a method for preparing the H substance comprises steps of: mixing the fruits and the vegetables, a bacteria liquid of Lactobacillus acidophilus, the bacteria liquid of the Bifidobacterium longum, the bacteria liquid of the Lactobacillus delbrueckii subsp bulgaricus and the bacteria liquid of the Streptococcus thermophilus, fermenting for obtaining a fermentation product, wherein the fermentation product is the fermentation conversion solution of the fruits and vegetables. mixing the following eight substances for obtaining a mixture, the mixture is the pharmaceutical composition for relieving the fatigue: A. ethanol extract of ginseng; B. ethanol extract of astragalus; C. ethanol extract of epimedium; D. ethanol extract of yam; E. ethanol extract of polygonatum; F. ethanol extract of dodder; G. aqueous extract of wolfberry; H. a fermentation conversion solution of fruits and vegetables; A. ethanol extract of ginseng; B. ethanol extract of astragalus; C. ethanol extract of epimedium; D. ethanol extract of yam; E. ethanol extract of polygonatum; F. ethanol extract of dodder; G. aqueous extract of wolfberry; H. a fermentation conversion solution of fruits and vegetables; wherein a method for preparing the A substance comprises: processing reflux extraction on the ginseng with an aqueous solution of ethanol for obtaining the extract, wherein the extract is the ethanol extract of the ginseng; wherein a method for preparing the B substance comprises: processing the reflux extraction on the astragalus with the aqueous solution of the ethanol for obtaining the extract, wherein the extract is the ethanol extract of the astragalus; wherein a method for preparing the C substance comprises: processing the reflux extraction on the epimedium with the aqueous solution of the ethanol for obtaining the extract, wherein the extract is the ethanol extract of the epimedium; wherein a method for preparing the D substance comprises: processing the reflux extraction on the yam with the aqueous solution of the ethanol for obtaining the extract, wherein the extract is the ethanol extract of the yam; wherein a method for preparing the E substance comprises: processing the reflux extraction on the polygonatum with the aqueous solution of the ethanol for obtaining the extract, wherein the extract is the ethanol extract of the polygonatum; wherein a method for preparing the F substance comprises: processing the reflux extraction on the dodder with the aqueous solution of the ethanol for obtaining the extract, wherein the extract is the ethanol extract of the dodder; wherein a method for preparing the G substance comprises: processing heating extraction on the wolfberry with pure water for obtaining the extract, wherein the extract is the aqueous extract of the wolfberry; wherein a method for preparing the H substance comprises steps of: mixing the fruits and the vegetables, a bacteria liquid of Lactobacillus acidophilus, the bacteria liquid of the Bifidobacterium longum, the bacteria liquid of the Lactobacillus delbrueckii subsp bulgaricus and the bacteria liquid of the Streptococcus thermophilus, fermenting for obtaining a fermentation product, wherein the fermentation product is the fermentation conversion solution of the fruits and vegetables.