Chromatography device, comprising at least: - a functional substrate, including a channel extending between a first and a second orifice opening onto a first face of the functional substrate, the channel comprising an inner wall covered with a functionalisation layer, - said first face of the functional substrate being disposed against a first face of a connection substrate, including a third and a fourth orifice opening onto said first face of the connection substrate, so that the first and second orifice are disposed respectively facing the third and fourth orifice, - the connection substrate being connected to a first and a second capillary respectively glued in a first and a second connection zone, the first and second connection zone opening onto an edge of the connection substrate, the connection substrate including a first fluid circuit extending between the first capillary and the third orifice and including a second fluid circuit extending between the second capillary and the fourth orifice, the connection substrate including a hood or cover assembled onto a part of the connection substrate in which the fluid circuits (15, 17) are made to close in a sealed manner the fluid connection zones (9-1, 9-2) and the fluid circuits (15, 17); and - a maintaining means (19, 21, 51) adapted to maintain in a temporary manner said first face of the functional substrate bearing against said first face of the connection substrate, so as to allow the sealed passage of a fluid between the first and second capillaries, through the first fluid circuit, the channel of the functional substrate and the second fluid circuit. - a functional substrate, including a channel extending between a first and a second orifice opening onto a first face of the functional substrate, the channel comprising an inner wall covered with a functionalisation layer, - said first face of the functional substrate being disposed against a first face of a connection substrate, including a third and a fourth orifice opening onto said first face of the connection substrate, so that the first and second orifice are disposed respectively facing the third and fourth orifice, - the connection substrate being connected to a first and a second capillary respectively glued in a first and a second connection zone, the first and second connection zone opening onto an edge of the connection substrate, the connection substrate including a first fluid circuit extending between the first capillary and the third orifice and including a second fluid circuit extending between the second capillary and the fourth orifice, the connection substrate including a hood or cover assembled onto a part of the connection substrate in which the fluid circuits (15, 17) are made to close in a sealed manner the fluid connection zones (9-1, 9-2) and the fluid circuits (15, 17); and - a maintaining means (19, 21, 51) adapted to maintain in a temporary manner said first face of the functional substrate bearing against said first face of the connection substrate, so as to allow the sealed passage of a fluid between the first and second capillaries, through the first fluid circuit, the channel of the functional substrate and the second fluid circuit.