A method for monitoring depletion yield of high-abundance proteins and recovery yield of low-abundance proteins from blood in real time, comprising: (a) labeling high-abundance proteins of a blood specimen with a fluorescent or UV marker which is selected from the group consisting of FITC (Fluorescein isothiocyanate), TRITC (tetramethyl-rhodamine isothiocyanate), Cy3, Cy5 and Rhodamine and spiking low-abundance proteins of a blood specimen with a fluorescent or UV marker which is selected from the group consisting of green fluorescent protein, modified green fluorescent protein (mGFP), enhanced green fluorescent protein (EGFP), red fluorescent protein, modified red fluorescent protein (mRFP), enhanced red fluorescent protein (ERFP), blue fluorescent protein, enhanced blue fluorescent protein (EBFP), yellow fluorescent protein, enhanced yellow fluorescent protein (EYFP), cyan fluorescent protein and enhanced cyan fluorescent protein (ECFP); and (b) passing blood samples containing the high-abundance proteins to be depleted which are labelled with said fluorescent or UV marker which is selected from the group consisting of FITC (Fluorescein isothiocyanate), TRITC (tetramethyl-rhodamine isothiocyanate), Cy3, Cy5 and Rhodamine and low-abundance proteins which are spiked with said fluorescent or UV marker which is selected from the group consisting of green fluorescent protein, modified green fluorescent protein (mGFP), enhanced green fluorescent protein (EGFP), red fluorescent protein, modified red fluorescent protein (mRFP), enhanced red fluorescent protein (ERFP), blue fluorescent protein, enhanced blue fluorescent protein (EBFP), yellow fluorescent protein, enhanced yellow fluorescent protein (EYFP), cyan fluorescent protein and enhanced cyan fluorescent protein (ECFP) through a removal column. (a) labeling high-abundance proteins of a blood specimen with a fluorescent or UV marker which is selected from the group consisting of FITC (Fluorescein isothiocyanate), TRITC (tetramethyl-rhodamine isothiocyanate), Cy3, Cy5 and Rhodamine and spiking low-abundance proteins of a blood specimen with a fluorescent or UV marker which is selected from the group consisting of green fluorescent protein, modified green fluorescent protein (mGFP), enhanced green fluorescent protein (EGFP), red fluorescent protein, modified red fluorescent protein (mRFP), enhanced red fluorescent protein (ERFP), blue fluorescent protein, enhanced blue fluorescent protein (EBFP), yellow fluorescent protein, enhanced yellow fluorescent protein (EYFP), cyan fluorescent protein and enhanced cyan fluorescent protein (ECFP); and (b) passing blood samples containing the high-abundance proteins to be depleted which are labelled with said fluorescent or UV marker which is selected from the group consisting of FITC (Fluorescein isothiocyanate), TRITC (tetramethyl-rhodamine isothiocyanate), Cy3, Cy5 and Rhodamine and low-abundance proteins which are spiked with said fluorescent or UV marker which is selected from the group consisting of green fluorescent protein, modified green fluorescent protein (mGFP), enhanced green fluorescent protein (EGFP), red fluorescent protein, modified red fluorescent protein (mRFP), enhanced red fluorescent protein (ERFP), blue fluorescent protein, enhanced blue fluorescent protein (EBFP), yellow fluorescent protein, enhanced yellow fluorescent protein (EYFP), cyan fluorescent protein and enhanced cyan fluorescent protein (ECFP) through a removal column.