A system for detection of an analyte in a sample, the system comprising: an analyte detection instrument; and one or more analyte detection cartridges, wherein the analyte detection instrument comprises: a control unit; a detection unit; and a cartridge interface for operable connection to the one or more analyte detection cartridges, wherein the control unit is configured for controlling activation of a plurality of electrodes in a cartridge for moving a droplet present in the cartridge, wherein the detection unit is configured to detect: i) a first analyte related signal from a droplet in a cartridge; and ii) a second analyte related signal from a tag or an analyte-specific binding member translocating through a pore of a nanopore layer in a cartridge, wherein each of the one or more analyte detection cartridges comprises: a plurality of electrodes to generate electrical actuation forces on a liquid droplet in response to the control unit; a first detection region for generating the first analyte related signal from a droplet in the cartridge; and a second detection region for generating the second analyte related signal from a tag or an analyte-specific binding member translocating through a pore of a nanopore layer in the cartridge. an analyte detection instrument; and one or more analyte detection cartridges, wherein the analyte detection instrument comprises: a control unit; a detection unit; and a cartridge interface for operable connection to the one or more analyte detection cartridges, a control unit; a detection unit; and a cartridge interface for operable connection to the one or more analyte detection cartridges, wherein the control unit is configured for controlling activation of a plurality of electrodes in a cartridge for moving a droplet present in the cartridge, wherein the detection unit is configured to detect: i) a first analyte related signal from a droplet in a cartridge; and ii) a second analyte related signal from a tag or an analyte-specific binding member translocating through a pore of a nanopore layer in a cartridge, i) a first analyte related signal from a droplet in a cartridge; and ii) a second analyte related signal from a tag or an analyte-specific binding member translocating through a pore of a nanopore layer in a cartridge, wherein each of the one or more analyte detection cartridges comprises: a plurality of electrodes to generate electrical actuation forces on a liquid droplet in response to the control unit; a first detection region for generating the first analyte related signal from a droplet in the cartridge; and a second detection region for generating the second analyte related signal from a tag or an analyte-specific binding member translocating through a pore of a nanopore layer in the cartridge. a plurality of electrodes to generate electrical actuation forces on a liquid droplet in response to the control unit; a first detection region for generating the first analyte related signal from a droplet in the cartridge; and a second detection region for generating the second analyte related signal from a tag or an analyte-specific binding member translocating through a pore of a nanopore layer in the cartridge.