A construction method of an immortal hepatic progenitor cell-like cell bank, which is characterized by comprising: S1: providing human primary hepatocyte cultures from different donor sources, transformation-culturing the human primary hepatocyte culture of each of the donor sources at a seeding density of 0.5×10 4 -5×10 4 cells/cm 2 for 7-14 days using TEM medium as a proliferation selection medium to obtain hepatic progenitor cell-like cell line, and performing cryopreservation treatment to the hepatic progenitor cell-like cell line of each of the donor sources obtained after the transformation-culture to obtain a heterogeneous hepatic progenitor cell-like cell bank; S2: thawing and proliferation-culturing the hepatic progenitor cell-like cell line of each of the donor sources to obtain adherent cells of the different donor sources respectively, wherein the confluence rate of the adherent cells of each of the donor sources is 70%-90%; S3: performing a first subculture treatment and virus infection successively at a seeding density of 2×10 4 -4×10 4 cells/cm 2 to the adherent cells of each of the donor sources wherein the virus is a lentivirus expressing the HPV E6E7 gene, and replacing the medium during the virus infection, wherein the medium of the culture obtained after the first subculture treatment is replaced at 6-12 hours after adding a DMEM/12 medium, lentivirus and a polybrene suspension, and then the culture is continually cultured for 24-72 hours to complete the virus infection, wherein the ratio of the number of the lentivirus to the adherent cells is 0.5-60; S4: performing a second subculture treatment at a seeding density of 2×10 4 -4×10 4 cells/cm 2 for 5-7 days with a passage number of 2 or 3 to the culture of each of the donor sources obtained after the virus infection, and continuously selection-culturing by adding TEM medium as a proliferation selection medium to the culture obtained after the second subculture treatment to obtain infected cultures of the hepatic progenitor cell-like cell lines from the different donor sources and the medium is replaced every 2-3 days during the second subculture treatment; S5: continuously-subculturing the infected culture of each of the donor sources at a seeding density of 2×10 4 -4×10 4 cells/cm 2 with a passage ratio of 1:2-1:4 and a passage number of 5-10 to obtain different immortal hepatic progenitor cell-like cell lines which will form the immortal hepatic progenitor cell-like cell bank. S1: providing human primary hepatocyte cultures from different donor sources, transformation-culturing the human primary hepatocyte culture of each of the donor sources at a seeding density of 0.5×10 4 -5×10 4 cells/cm 2 for 7-14 days using TEM medium as a proliferation selection medium to obtain hepatic progenitor cell-like cell line, and performing cryopreservation treatment to the hepatic progenitor cell-like cell line of each of the donor sources obtained after the transformation-culture to obtain a heterogeneous hepatic progenitor cell-like cell bank; S2: thawing and proliferation-culturing the hepatic progenitor cell-like cell line of each of the donor sources to obtain adherent cells of the different donor sources respectively, wherein the confluence rate of the adherent cells of each of the donor sources is 70%-90%; S3: performing a first subculture treatment and virus infection successively at a seeding density of 2×10 4 -4×10 4 cells/cm 2 to the adherent cells of each of the donor sources wherein the virus is a lentivirus expressing the HPV E6E7 gene, and replacing the medium during the virus infection, wherein the medium of the culture obtained after the first subculture treatment is replaced at 6-12 hours after adding a DMEM/12 medium, lentivirus and a polybrene suspension, and then the culture is continually cultured for 24-72 hours to complete the virus infection, wherein the ratio of the number of the lentivirus to the adherent cells is 0.5-60; S4: performing a second subculture treatment at a seeding density of 2×10 4 -4×10 4 cells/cm 2 for 5-7 days with a passage number of 2 or 3 to the culture of each of the donor sources obtained after the virus infection, and continuously selection-culturing by adding TEM medium as a proliferation selection medium to the culture obtained after the second subculture treatment to obtain infected cultures of the hepatic progenitor cell-like cell lines from the different donor sources and the medium is replaced every 2-3 days during the second subculture treatment; S5: continuously-subculturing the infected culture of each of the donor sources at a seeding density of 2×10 4 -4×10 4 cells/cm 2 with a passage ratio of 1:2-1:4 and a passage number of 5-10 to obtain different immortal hepatic progenitor cell-like cell lines which will form the immortal hepatic progenitor cell-like cell bank.