A vertical centrifugal separation apparatus comprising: a cylindrical rotational tube which has a supply port for a liquid to be processed formed on a lower side, has a discharge port for a centrifuged liquid formed on an upper side, and is rotated about a vertical axis in performing centrifugation; a casing which accommodates the cylindrical rotational tube such that the tube is rotatable about the vertical axis; and a support apparatus which supports the casing and the cylindrical rotational tube such that the casing and the tube are overturned together, wherein the support apparatus can be set to a first overturn position in which a centrifuged liquid remaining in the cylindrical rotational tube is recovered after the centrifugation is completed and a second overturn position in which the cylindrical rotational tube is pulled out of the casing after the remaining centrifuged liquid is recovered; wherein the apparatus is characterized in that it comprises a self-sealing apparatus which closes the supply port for the liquid to be processed when the rotation of the cylindrical rotational tube about the vertical axis is stopped, and includes a supply hole for the liquid to be processed and a close member which covers the supply hole from the outside to close the hole, the hole being provided in a circumferential direction in a lower portion of the cylindrical rotational tube and passing through the cylindrical rotational tube in a diameter direction, and wherein the close member expands or moves outward to open the supply hole by the action of a centrifugal force when the cylindrical rotational tube is rotated in performing the centrifugation. a cylindrical rotational tube which has a supply port for a liquid to be processed formed on a lower side, has a discharge port for a centrifuged liquid formed on an upper side, and is rotated about a vertical axis in performing centrifugation; a casing which accommodates the cylindrical rotational tube such that the tube is rotatable about the vertical axis; and a support apparatus which supports the casing and the cylindrical rotational tube such that the casing and the tube are overturned together, wherein the support apparatus can be set to a first overturn position in which a centrifuged liquid remaining in the cylindrical rotational tube is recovered after the centrifugation is completed and a second overturn position in which the cylindrical rotational tube is pulled out of the casing after the remaining centrifuged liquid is recovered; wherein the apparatus is characterized in that it comprises a self-sealing apparatus which closes the supply port for the liquid to be processed when the rotation of the cylindrical rotational tube about the vertical axis is stopped, and includes a supply hole for the liquid to be processed and a close member which covers the supply hole from the outside to close the hole, the hole being provided in a circumferential direction in a lower portion of the cylindrical rotational tube and passing through the cylindrical rotational tube in a diameter direction, and wherein the close member expands or moves outward to open the supply hole by the action of a centrifugal force when the cylindrical rotational tube is rotated in performing the centrifugation.