mhurhangee/ep-ind-claims huggingface dataset:
A method for modifying a target genomic locus in a mammalian cell, comprising: (a) introducing into the cell a nuclease agent that makes a single or double-strand break within the target genomic locus; (b) introducing into the cell a first large targeting vector (LTVEC) that is at least 10 kb in length and comprises a first nucleic acid insert flanked by a first 5' homology arm and a first 3' homology arm, and a second LTVEC that is at least 10 kb in length and comprises a second nucleic acid insert flanked by a second 5' homology arm and a second 3' homology arm, wherein the combined size of the first nucleic acid insert and the second nucleic acid insert is at least 100 kb, wherein the first 3' homology arm of the first LTVEC has a first overlapping sequence homologous to the second 5' homology arm of the second LTVEC, and the first 5' homology arm of the first LTVEC and the second 3' homology arm of the second LTVEC are homologous to corresponding genomic segments within the target genomic locus, wherein the first overlapping sequence is at least 1 kb, and wherein the target genomic locus is modified by integration of the first nucleic acid insert and the second nucleic acid insert between the corresponding genomic segments; and (c) selecting a targeted cell comprising the first nucleic acid insert and the second nucleic acid insert integrated into the target genomic locus. (a) introducing into the cell a nuclease agent that makes a single or double-strand break within the target genomic locus; (b) introducing into the cell a first large targeting vector (LTVEC) that is at least 10 kb in length and comprises a first nucleic acid insert flanked by a first 5' homology arm and a first 3' homology arm, and a second LTVEC that is at least 10 kb in length and comprises a second nucleic acid insert flanked by a second 5' homology arm and a second 3' homology arm, wherein the combined size of the first nucleic acid insert and the second nucleic acid insert is at least 100 kb, wherein the first 3' homology arm of the first LTVEC has a first overlapping sequence homologous to the second 5' homology arm of the second LTVEC, and the first 5' homology arm of the first LTVEC and the second 3' homology arm of the second LTVEC are homologous to corresponding genomic segments within the target genomic locus, wherein the first overlapping sequence is at least 1 kb, and wherein the target genomic locus is modified by integration of the first nucleic acid insert and the second nucleic acid insert between the corresponding genomic segments; and (c) selecting a targeted cell comprising the first nucleic acid insert and the second nucleic acid insert integrated into the target genomic locus.
A coolant pump for an internal combustion engine, having a drive shaft, a coolant pump impeller which is arranged at said drive shaft at least in a rotationally fixed manner and by means of which a coolant is adapted to be delivered, an adjustable control slide by means of which a throughflow cross-section of an annular gap between an outlet of said coolant pump impeller and the surrounding delivery duct is adapted to be controlled, a control pump having a control pump impeller which is arranged at said drive shaft at least in a rotationally fixed manner, a flow duct of said control pump in which a pressure is adapted to be generated by rotation of said control pump impeller, a pressure duct via which an outlet of said flow duct is adapted to be fluidically connected to a first pressure chamber of said control slide, which is formed on the axial side of said control slide facing away from said coolant pump impeller, a valve via which a throughflow cross-section of said pressure duct is adapted to be closed and opened, characterized in that said flow duct is fluidically connected via a connecting duct to a second pressure chamber of said control slide, which is formed on the axial side of said control slide facing said coolant pump impeller.
Device for the production of a casing of increased length for food items, in particular for sausages, from a multiplicity of individual casing sections of gut that contains collagens, such as natural gut or collagen-containing artificial gut, comprising a mandrel and at least two casing sections, wherein the casing sections are slid over the mandrel such that they create an overlapping region and are disposed overlapping one another, and with a heating element for the temporary exchange of heat between heating element and the overlapping region such that the casing sections are at least to some extent fused together in the overlapping region, and with a deformable expansion body, wherein this expansion body is implemented such that it encompasses the mandrel annularly and is disposed within the casing sections, wherein the deformable expansion body is comprised of an elastically deformable material, and with a forming mold sectionally enveloping the mandrel, characterized in that as the heating element a heating filament or a heating foil is provided, wherein the heating element is disposed between the forming mold and the overlapping region.
A method for scanning a surface of a workpiece using a scanning probe mounted on a support on a coordinate measuring machine, said support containing drive means (5,7) for actuating the movement of said scanning probe relatively to said support, control means coupled to the drive means, and memory means for storing theoretical profiles and coordinates of said surface, said method comprising the steps of: determine a first range of values for a contact force F applied between the tip of said scanning probe and said surface; operate said drive means (5,7) to position said tip in contact with said surface; operate said coordinate measuring machine to move the support along a determined trajectory; operate said drive means (5,7) to produce, simultaneously with the relative movement of the support with respect to the surface, movements of said scanning probe relative to the support; whereby said control means adjust the actuation of the drive means (5,7) along a scanning path in order to maintain said contact force F within said first range of values during the whole scanning operation along said scanning path; said method being characterized in that it further comprises the initial step of: (i) loading said theoretical profile of said surface to scan into said memory means; and in that said control means perform the adjustment of the actuation of said drive means along said path according to said theoretical profile. determine a first range of values for a contact force F applied between the tip of said scanning probe and said surface; operate said drive means (5,7) to position said tip in contact with said surface; operate said coordinate measuring machine to move the support along a determined trajectory; operate said drive means (5,7) to produce, simultaneously with the relative movement of the support with respect to the surface, movements of said scanning probe relative to the support; (i) loading said theoretical profile of said surface to scan into said memory means;
A sterility verification method for verifying sterility of a container with use of a content filling system having a container sterilizer which sterilizes the container, and a filler which fills a content in the container, the method comprising: a step of performing a test individually as to whether or not sterility is ensured for each element of the content filling system; a step of filling a culture medium, pH of which is previously adjusted according to pH of the content, in the container with the use of the filler, after the step of performing the test; a step of capping the container filled with the culture medium with a cap; a step of tilting or reversing the container filled with the culture medium before culturing the culture medium, to reliably bring the culture medium into contact with an inner surface of the container; and a step of verifying whether or not the bacteria survive or propagate in the culture medium in the container. a step of performing a test individually as to whether or not sterility is ensured for each element of the content filling system; a step of filling a culture medium, pH of which is previously adjusted according to pH of the content, in the container with the use of the filler, after the step of performing the test; a step of capping the container filled with the culture medium with a cap; a step of tilting or reversing the container filled with the culture medium before culturing the culture medium, to reliably bring the culture medium into contact with an inner surface of the container; and a step of verifying whether or not the bacteria survive or propagate in the culture medium in the container.
An electric storage apparatus comprising a plurality of electric storage devices that are arranged side by side in electric connection with one another, wherein each electric storage device is comprising: a cuboid case having a plurality of walls that includes a long side wall, a short side wall and a bottom wall; an electric storage element in the case and away from an inner surface of at least one wall of the plurality of walls; and a heat transfer member (125, 165) in contact with an outer surface of the bottom wall, the inner surface of which is away from the electric storage element; wherein the inner surface of the bottom wall has a shorter distance apart from the electric storage element than that of the short side wall; and wherein the bottom wall is configured to resist expansion even if the electric storage element expands or an internal pressure of the electric storage device increases. a cuboid case having a plurality of walls that includes a long side wall, a short side wall and a bottom wall; an electric storage element in the case and away from an inner surface of at least one wall of the plurality of walls; and a heat transfer member (125, 165) in contact with an outer surface of the bottom wall, the inner surface of which is away from the electric storage element; wherein the inner surface of the bottom wall has a shorter distance apart from the electric storage element than that of the short side wall; and wherein the bottom wall is configured to resist expansion even if the electric storage element expands or an internal pressure of the electric storage device increases.
A cutting insert comprising a first end surface whose contour shape is substantially polygonal, a second end surface opposing the first end surface, and a peripheral side surface extending between these end surfaces (2, 3), wherein: a cutting edge is formed at least in part of an intersecting edge between the first end surface and the peripheral side surface; the cutting edge comprises a corner cutting edge, a first cutting edge, a second cutting edge formed on the opposite side of the first cutting edge with respect to the corner cutting edge, and a wiper edge formed between the second cutting edge and the corner cutting edge; in the peripheral side surface, when regarding a side surface part leading to the wiper edge as a wiper-edge side surface, a side surface part leading to the second cutting edge as a side surface and a direction perpendicular to the second cutting edge as a direction V as viewed from a direction facing the first end surface; and the cutting edge comprises a second wiper edge formed between the first cutting edge and the corner cutting edge; characterised in that, in a side view in the direction V, a boundary of the wiper-edge side surface which is close to the second side surface is inclined in a direction perpendicular to the first end surface, wherein: in the peripheral side surface, when regarding a side surface part leading to the second wiper edge as a second wiper-edge side surface, the second wiper-edge side surface is gradually inclined inward, heading away from the first end surface.. a cutting edge is formed at least in part of an intersecting edge between the first end surface and the peripheral side surface; the cutting edge comprises a corner cutting edge, a first cutting edge, a second cutting edge formed on the opposite side of the first cutting edge with respect to the corner cutting edge, and a wiper edge formed between the second cutting edge and the corner cutting edge; in the peripheral side surface, when regarding a side surface part leading to the wiper edge as a wiper-edge side surface, a side surface part leading to the second cutting edge as a side surface and a direction perpendicular to the second cutting edge as a direction V as viewed from a direction facing the first end surface; and in the peripheral side surface, when regarding a side surface part leading to the second wiper edge as a second wiper-edge side surface, the second wiper-edge side surface is gradually inclined inward, heading away from the first end surface..
A system for receiving, transferring, and storing solar thermal energy, comprising: a concentrating solar energy collector; a light transfer conduit; a thermal storage material; and an insulated container, the insulated container containing the thermal storage material, the light transfer conduit being configured to transfer solar energy collected by the solar energy collector to the thermal storage material through a wall of the insulated container, wherein the light transfer conduit includes: an input port receiving the solar energy; an exit port proximal to the thermal storage material, and wherein the light transfer conduit is configured to transmit the solar energy substantially losslessly and to radiate the solar energy into the thermal storage material via the exit port. a concentrating solar energy collector; a light transfer conduit; a thermal storage material; and an insulated container, the insulated container containing the thermal storage material, the light transfer conduit being configured to transfer solar energy collected by the solar energy collector to the thermal storage material through a wall of the insulated container, wherein the light transfer conduit includes: an input port receiving the solar energy; an exit port proximal to the thermal storage material, and an input port receiving the solar energy; an exit port proximal to the thermal storage material, and wherein the light transfer conduit is configured to transmit the solar energy substantially losslessly and to radiate the solar energy into the thermal storage material via the exit port.
Injection moulding machine for processing plastics and other plasticizable materials such as powdered, metallic or ceramic compositions, having - at least one mould closing unit, - at least one injection moulding unit, - a plurality of actuating units for the operation of the mould closing unit and the injection moulding unit, which actuating units comprise, at least at one lubrication site, mechanically cooperating components which are movable relative to one another and which are to be supplied with a lubricant, wherein the components are connected in the region of the lubrication site at mutually spaced sites, firstly, to the surroundings in an open connection and, secondly, to a negative pressure generator, characterised in that in order to supply the lubrication site with lubricant, an open lubricant circuit is provided which comprises a lubricant pump and the negative pressure generator which is connected to the lubrication site and by means of which the excess lubricant is conducted away. - at least one mould closing unit, - at least one injection moulding unit, - a plurality of actuating units for the operation of the mould closing unit and the injection moulding unit, which actuating units comprise, at least at one lubrication site, mechanically cooperating components which are movable relative to one another and which are to be supplied with a lubricant,
A method for repairing a vane of a turbomachine propeller, in particular a turbomachine fan, this vane comprising a blade made from an organic-matrix composite and a metal shield bonded to a leading edge of the blade, the metal shield having a damaged region, the method comprises a step of refilling the damaged region, by adding a metallic material to this damaged region and laser melting the material, and a step of adjusting the damaged region by machining the molten material, characterised in that prior to said refilling and adjusting steps, the method comprises a step of resetting the leading edge of the blade in order to measure a real profile of said damaged region on said leading edge, and a step of cooling the entire blade.
Bale measuring method for a rectangular baler having a bale chamber in which bales are formed, comprising the steps of: - measuring a movement of crop material downstream of the bale chamber as the crop material moves backward in the baler; - using the measured movement (L M) of the crop material downstream of the bale chamber to calibrate a measurement and calculation tool (11, 12, 13) for measuring a movement of crop material in the bale chamber and for calculating a length of a bale being formed in the bale chamber based on said measurement; and - determining the length of a bale being formed in the bale chamber (L B) using the calibrated measurement and calculation tool (11, 12, 13). - measuring a movement of crop material downstream of the bale chamber as the crop material moves backward in the baler; - using the measured movement (L M) of the crop material downstream of the bale chamber to calibrate a measurement and calculation tool (11, 12, 13) for measuring a movement of crop material in the bale chamber and for calculating a length of a bale being formed in the bale chamber based on said measurement; and - determining the length of a bale being formed in the bale chamber (L B) using the calibrated measurement and calculation tool (11, 12, 13).
Flow rate regulator (1, 110) having a regulator housing that has at least one regulator annular duct in which there is provided an annular throttle body composed of elastic material, which throttle body delimits a control gap between itself and a duct wall which bears a regulating profiling, the passage cross section of which control gap can be varied by the throttle body that deforms under the pressure difference that is generated as flow passes through, and having at least one valve that has a valve body which, under the pressure of the medium flowing through, moves from an open position into a closed position counter to a restoring force, in which closed position the valve body closes off at least one valve opening, characterized in that the valve body is provided in a valve annular duct of the regulator housing, which valve annular duct has at least one valve opening in the region of its duct base, in that the valve body is of annular configuration and is produced from elastic material, in that the annular valve body deforms under the pressure of the medium flowing through such that the valve body moves from the open position into the closed position counter to the restoring force of the inherent elasticity of the elastic material used for the valve body, in which closed position the elastic valve body sealingly closes the at least one valve opening, such that, above a certain pressure of the inflowing fluid, no further fluid volume flowing through the valve is to be expected, and in that the curve profile (b), plotted as a function of the pressure of the inflowing fluid, of the throughflow volume flowing through the at least one regulator annular duct per unit of time and the curve profile (a) of the additional volume flowing through the at least one valve opening are added together such that the flow rate regulator (1, 110) attains and maintains its desired maximum value of the throughflow rate already in the presence of low pressures of the inflowing fluid.
A glass panel comprising at least one glass sheet, at least one of the faces of which has at least one rough area comprising corundum particles embedded in a matrix based on a glass frit, characterized in that said particles have an untamped bulk density/true density ratio at least equal to 0.425, the untamped bulk density being measured according to the ANSI B74.4 standard using the "A" installation, a particle size distribution such that d 90 is less than or equal to 130 μm, and a porosity of less than 50%, the glass frit being lead-free and comprising silica SiO 2 bismuth oxide Bi 2 O 3 and/or zinc oxide ZnO, in the following proportions (in % by weight): SiO 2 35 - 75% Bi 2 O 3 20 - 40%, preferably 25 - 30% ZnO 4 - 10%.
A method of regulating cooking procedures of a cooking stuff in a cooking chamber of a cooking appliance with heating means, preferably a baking oven, comprising the following steps: a) input of a cooking stuff to be cooked by an operator into a controller of said cooking appliance, b) detection of a concentration of a gas or of a moisture (f) escaping from said cooking stuff in said cooking chamber, once said cooking stuff has been introduced, by a sensor over time (t), c) obtaining of a gradient (f) of a progression of said detected concentration (f) over the course of time (t), d) reading out of a trigger value (f' trigger) linked to said cooking stuff to be cooked for said gradient (f) from a memory means of said controller, e) determination of the time (T(f' trigger)) at which said trigger value (f' trigger) is reached or fallen below, characterized by the further steps: f) obtaining of a run-on time (T add) linked to a time (T(f' trigger)) at which said trigger value (f' trigger) is reached for said cooking stuff from said memory means at said time at which said trigger value (f' trigger) is reached, a period of the run-on time (T add) being a function of said time (T(f' trigger)) at which said trigger value (f' trigger) is reached, g) start of said run-on time (T add), h) continuation of said cooking procedure as run-on process until said run-on time (T add) has elapsed for the case that said value remains below said trigger value (f' trigger). a) input of a cooking stuff to be cooked by an operator into a controller of said cooking appliance, b) detection of a concentration of a gas or of a moisture (f) escaping from said cooking stuff in said cooking chamber, once said cooking stuff has been introduced, by a sensor over time (t), c) obtaining of a gradient (f) of a progression of said detected concentration (f) over the course of time (t), d) reading out of a trigger value (f' trigger) linked to said cooking stuff to be cooked for said gradient (f) from a memory means of said controller, e) determination of the time (T(f' trigger)) at which said trigger value (f' trigger) is reached or fallen below, characterized by the further steps: f) obtaining of a run-on time (T add) linked to a time (T(f' trigger)) at which said trigger value (f' trigger) is reached for said cooking stuff from said memory means at said time at which said trigger value (f' trigger) is reached, a period of the run-on time (T add) being a function of said time (T(f' trigger)) at which said trigger value (f' trigger) is reached, g) start of said run-on time (T add), h) continuation of said cooking procedure as run-on process until said run-on time (T add) has elapsed for the case that said value remains below said trigger value (f' trigger).
A peptide for use in a method of treatment of an animal, said peptide having an amino acid sequence including an amino acid sequence selected from the group consisting of: (a) SEQ ID NO:4; and (b) SEQ ID NO:6. (a) SEQ ID NO:4; and (b) SEQ ID NO:6.