A quantitative uniplex method for detecting the presence of one or more of at least six respiratory infection-causing microorganisms in a sample or detecting the absence of said respiratory infection-causing microorganisms in said sample, said method comprising: A) applying a sample to a test card, wherein said test card comprises six discrete wells: 1) a first well that includes a first probe, wherein the first probe has a nucleic acid sequence that comprises a nucleic acid sequence having at least 90% sequence identity to the nucleic acid sequence GGCAATGCWG (SEQ ID NO: 1); 2) a second well that includes a second probe, wherein the second probe has a nucleic acid sequence that comprises a nucleic acid sequence having at least 90% sequence identity to the nucleic acid sequence GAYGGGACCR (SEQ ID NO: 2); 3) a third well that includes a third probe, wherein the third probe has a nucleic acid sequence that comprises a nucleic acid sequence having at least 90% sequence identity to the nucleic acid sequence CACCAGACAC (SEQ ID NO: 3); 4) a fourth well that includes a fourth probe, wherein the fourth probe has a nucleic acid sequence that comprises a nucleic acid sequence having at least 90% sequence identity to the nucleic acid sequence GGTCATTGGR (SEQ ID NO: 4); 5) a fifth well that includes a fifth probe, wherein the fifth probe has a nucleic acid sequence that comprises a nucleic acid sequence having at least 90% sequence identity to the nucleic acid sequence CACTGGGCAC (SEQ ID NO: 5); 6) a sixth well that includes a sixth probe, wherein the sixth probe has a nucleic acid sequence that comprises a nucleic acid sequence having at least 90% sequence identity to the nucleic acid sequence RGTGTTCATT (SEQ ID NO: 6); B) allowing nucleic acid present in the sample to contact with the probes within said wells; C) detecting for the presence of bound nucleic acid complex in which sample nucleic acid has bound to one or more of said probes; wherein the presence of bound nucleic acid complex confirms that nucleic acid from one or more of said six respiratory infection-causing microorganisms is present within the sample, and wherein the absence of bound nucleic acid complex confirms that nucleic acid from all of said six respiratory infection-causing microorganisms is absent within the sample; wherein, if present, the load of each of one or more of said six respiratory infection-causing microorganisms is determined. A) applying a sample to a test card, wherein said test card comprises six discrete wells: 1) a first well that includes a first probe, wherein the first probe has a nucleic acid sequence that comprises a nucleic acid sequence having at least 90% sequence identity to the nucleic acid sequence GGCAATGCWG (SEQ ID NO: 1); 2) a second well that includes a second probe, wherein the second probe has a nucleic acid sequence that comprises a nucleic acid sequence having at least 90% sequence identity to the nucleic acid sequence GAYGGGACCR (SEQ ID NO: 2); 3) a third well that includes a third probe, wherein the third probe has a nucleic acid sequence that comprises a nucleic acid sequence having at least 90% sequence identity to the nucleic acid sequence CACCAGACAC (SEQ ID NO: 3); 4) a fourth well that includes a fourth probe, wherein the fourth probe has a nucleic acid sequence that comprises a nucleic acid sequence having at least 90% sequence identity to the nucleic acid sequence GGTCATTGGR (SEQ ID NO: 4); 5) a fifth well that includes a fifth probe, wherein the fifth probe has a nucleic acid sequence that comprises a nucleic acid sequence having at least 90% sequence identity to the nucleic acid sequence CACTGGGCAC (SEQ ID NO: 5); 6) a sixth well that includes a sixth probe, wherein the sixth probe has a nucleic acid sequence that comprises a nucleic acid sequence having at least 90% sequence identity to the nucleic acid sequence RGTGTTCATT (SEQ ID NO: 6); 1) a first well that includes a first probe, wherein the first probe has a nucleic acid sequence that comprises a nucleic acid sequence having at least 90% sequence identity to the nucleic acid sequence GGCAATGCWG (SEQ ID NO: 1); 2) a second well that includes a second probe, wherein the second probe has a nucleic acid sequence that comprises a nucleic acid sequence having at least 90% sequence identity to the nucleic acid sequence GAYGGGACCR (SEQ ID NO: 2); 3) a third well that includes a third probe, wherein the third probe has a nucleic acid sequence that comprises a nucleic acid sequence having at least 90% sequence identity to the nucleic acid sequence CACCAGACAC (SEQ ID NO: 3); 4) a fourth well that includes a fourth probe, wherein the fourth probe has a nucleic acid sequence that comprises a nucleic acid sequence having at least 90% sequence identity to the nucleic acid sequence GGTCATTGGR (SEQ ID NO: 4); 5) a fifth well that includes a fifth probe, wherein the fifth probe has a nucleic acid sequence that comprises a nucleic acid sequence having at least 90% sequence identity to the nucleic acid sequence CACTGGGCAC (SEQ ID NO: 5); 6) a sixth well that includes a sixth probe, wherein the sixth probe has a nucleic acid sequence that comprises a nucleic acid sequence having at least 90% sequence identity to the nucleic acid sequence RGTGTTCATT (SEQ ID NO: 6); B) allowing nucleic acid present in the sample to contact with the probes within said wells; C) detecting for the presence of bound nucleic acid complex in which sample nucleic acid has bound to one or more of said probes;