Method for super-resolution 3D fluorescence microscopy, wherein - fluorescence emitters for emitting fluorescence radiation in a sample are repeatedly excited and individual images are generated from the sample using a microscope that has an imaging beam path with an optical resolution and a focal plane, wherein the fluorescence emitters are excited to emit fluorescence radiation such that at least a subset of the fluorescence emitters in each individual image is isolated such that the images of said fluorescence emitters are separable within the scope of the optical resolution in the individual images, - the locations of said fluorescence emitters are localized in the generated individual images from the images of the isolated fluorescence emitters with a spatial accuracy that goes beyond an optical resolution, and a super-resolved total image is generated therefrom, - each individual image is split, using a splitter element into a first and a second partial image (12, 13), wherein the first partial image images a first focal plane in the sample and the second partial image images a second focal plane in the sample, - both partial images (12, 13) are imaged separately, characterized in that - an adaptive mirror is used as the splitter element and is mounted substantially in a pupil of the imaging beam path, - the adaptive mirror is set such that it images the two partial images (12, 13) in a spatially separated fashion, - the adaptive mirror is set such that it implements two different focal lengths for the two partial images (12, 13), and - the adaptive mirror images the two partial images (12, 13) along diverging optical axes (OA1, OA2) onto adjacent image recording regions (Ka, Kb). - fluorescence emitters for emitting fluorescence radiation in a sample are repeatedly excited and individual images are generated from the sample using a microscope that has an imaging beam path with an optical resolution and a focal plane, wherein the fluorescence emitters are excited to emit fluorescence radiation such that at least a subset of the fluorescence emitters in each individual image is isolated such that the images of said fluorescence emitters are separable within the scope of the optical resolution in the individual images, - the locations of said fluorescence emitters are localized in the generated individual images from the images of the isolated fluorescence emitters with a spatial accuracy that goes beyond an optical resolution, and a super-resolved total image is generated therefrom, - each individual image is split, using a splitter element into a first and a second partial image (12, 13), wherein the first partial image images a first focal plane in the sample and the second partial image images a second focal plane in the sample, - both partial images (12, 13) are imaged separately, - an adaptive mirror is used as the splitter element and is mounted substantially in a pupil of the imaging beam path, - the adaptive mirror is set such that it images the two partial images (12, 13) in a spatially separated fashion, - the adaptive mirror is set such that it implements two different focal lengths for the two partial images (12, 13), and - the adaptive mirror images the two partial images (12, 13) along diverging optical axes (OA1, OA2) onto adjacent image recording regions (Ka, Kb).