A detection system for detecting presence or an amount of a detection object substance contained in a sample by utilizing surface plasmon resonance, comprising a detection apparatus and a detection chip (110, 210), the detection apparatus comprising: a chip holder (131, 231, 331, 431, 531) for positioning the detection chip (110, 210), the detection chip (110, 210) including a prism including an incidence surface, an emission surface, a film formation surface and a bottom surface, a metal film formed on the film formation surface, a capturing body disposed in a reaction site that is at least a part of a region on the metal film, and a base that forms, together with the metal film or the film formation surface, a liquid storage section that stores liquid; an excitation light irradiation section that emits excitation light toward a rear surface of the metal film through the incidence surface of the detection chip (110, 210) held in the chip holder (131, 231, 331, 431, 531), the rear surface corresponding to the reaction site; and a light detection section that detects fluorescence generated by a fluorescence material captured by the capturing body in the reaction site and excited by the excitation light, or detects reflection light of excitation light reflected by the metal film and emitted from the emission surface, wherein the chip holder (131, 231, 331, 431, 531) positions the detection chip (110, 210) by making contact with at least one of a bottom surface of the base and a reference surface disposed on the prism, or by holding at least one of the bottom surface and the reference surface; the chip holder comprises a pair of side plates such that a second gap is provided between each of the pair of side plates and any one of the incidence surface and emission surface of the detection chip (110, 210); characterized in that the chip holder (131, 231, 331, 431, 531) comprises: a bottom plate facing the bottom surface of the detection chip (110, 210) with a first gap; and the pair of side plates is disposed at the bottom plate, the detection apparatus comprises a heat source accommodated in the chip holder (131, 231, 331, 431, 531) so as to face either the bottom surface without making contact with the bottom surface or the incidence surface without making contact with incidence surface or the emission surface without making contact with emission surface, and a position of the heat source is such that the heat source does not interfere with a light path of the excitation light a chip holder (131, 231, 331, 431, 531) for positioning the detection chip (110, 210), the detection chip (110, 210) including a prism including an incidence surface, an emission surface, a film formation surface and a bottom surface, a metal film formed on the film formation surface, a capturing body disposed in a reaction site that is at least a part of a region on the metal film, and a base that forms, together with the metal film or the film formation surface, a liquid storage section that stores liquid; an excitation light irradiation section that emits excitation light toward a rear surface of the metal film through the incidence surface of the detection chip (110, 210) held in the chip holder (131, 231, 331, 431, 531), the rear surface corresponding to the reaction site; and a light detection section that detects fluorescence generated by a fluorescence material captured by the capturing body in the reaction site and excited by the excitation light, or detects reflection light of excitation light reflected by the metal film and emitted from the emission surface, wherein the chip holder (131, 231, 331, 431, 531) positions the detection chip (110, 210) by making contact with at least one of a bottom surface of the base and a reference surface disposed on the prism, or by holding at least one of the bottom surface and the reference surface; the chip holder comprises a pair of side plates such that a second gap is provided between each of the pair of side plates and any one of the incidence surface and emission surface of the detection chip (110, 210); characterized in that the chip holder (131, 231, 331, 431, 531) comprises: a bottom plate facing the bottom surface of the detection chip (110, 210) with a first gap; and the pair of side plates is disposed at the bottom plate, the detection apparatus comprises a heat source accommodated in the chip holder (131, 231, 331, 431, 531) so as to face either the bottom surface without making contact with the bottom surface or the incidence surface without making contact with incidence surface or the emission surface without making contact with emission surface, and a position of the heat source is such that the heat source does not interfere with a light path of the excitation light a bottom plate facing the bottom surface of the detection chip (110, 210) with a first gap; and the pair of side plates is disposed at the bottom plate, the detection apparatus comprises a heat source accommodated in the chip holder (131, 231, 331, 431, 531) so as to face either the bottom surface without making contact with the bottom surface or the incidence surface without making contact with incidence surface or the emission surface without making contact with emission surface, and a position of the heat source is such that the heat source does not interfere with a light path of the excitation light