A baker's yeast strain, wherein the strain is capable of surviving with a survival rate of at least 1 %, a regimen wherein a) the yeast strain is grown on YPD medium for 15 h in a shaker-incubator at 30°C and 200rpm, b) the yeast is harvested and re-suspended in fresh YPD medium to a concentration of 5 OD 600, c) 5 mL yeast suspensions in 50mL tubes are incubated at -20°C for 48-72 h, d) followed by four freezing/thawing cycles, each freezing/thawing cycle comprising 1.5 h at 30°C and 200 rpm, and 1 h at -20°C, wherein the survival rate is determined as the number of viable yeast colonies after said freeze/thawing cycles / number of viable yeast colonies before said freeze/thawing cycles x 100%, and wherein the number of viable yeast colonies is measured using seeding decimal dilutions of yeast suspensions on YPD agar plates and incubation at 30°C for 48 hours; wherein the strain comprises mutations in all alleles of at least two genes selected from the group consisting of: a) Gene YDL248W named COS7 located at Chromosome IV, wherein the mutation is a deletion downstream to position 2232; b) Gene YJR155W named AAD10 located at Chromosome X, wherein the mutation is a deletion downstream to position 727404; c) Gene YDL245C named HXT15 located at Chromosome IV, wherein the mutation is a deletion downstream to position 11657; d) Gene YJR151C named DAN4 located at Chromosome X, wherein the mutation is an introduction of at least one stop codon at amino acid position 342 and/or at amino acid position 53 and/or at least partial deletion and/or genomic rearrangement between coordinates of Chromosome X:714,902-715,267; and e) Gene YDR420W named HKR1 located at Chromosome IV, wherein the mutation is at least one point mutation leading to an amino acids exchange, wherein the mutation is selected from at least one of a group comprising a mutation at position 1308583 and at position 1308589, resulting in exchange from Serine to Proline and/or a mutation at position 1308951 and at position 1309390 resulting in exchange from Phenylalanine to Leucine. a) the yeast strain is grown on YPD medium for 15 h in a shaker-incubator at 30°C and 200rpm, b) the yeast is harvested and re-suspended in fresh YPD medium to a concentration of 5 OD 600, c) 5 mL yeast suspensions in 50mL tubes are incubated at -20°C for 48-72 h, d) followed by four freezing/thawing cycles, each freezing/thawing cycle comprising 1.5 h at 30°C and 200 rpm, and 1 h at -20°C, wherein the survival rate is determined as the number of viable yeast colonies after said freeze/thawing cycles / number of viable yeast colonies before said freeze/thawing cycles x 100%, and wherein the number of viable yeast colonies is measured using seeding decimal dilutions of yeast suspensions on YPD agar plates and incubation at 30°C for 48 hours; wherein the strain comprises mutations in all alleles of at least two genes selected from the group consisting of: a) Gene YDL248W named COS7 located at Chromosome IV, wherein the mutation is a deletion downstream to position 2232; b) Gene YJR155W named AAD10 located at Chromosome X, wherein the mutation is a deletion downstream to position 727404; c) Gene YDL245C named HXT15 located at Chromosome IV, wherein the mutation is a deletion downstream to position 11657; d) Gene YJR151C named DAN4 located at Chromosome X, wherein the mutation is an introduction of at least one stop codon at amino acid position 342 and/or at amino acid position 53 and/or at least partial deletion and/or genomic rearrangement between coordinates of Chromosome X:714,902-715,267; and e) Gene YDR420W named HKR1 located at Chromosome IV, wherein the mutation is at least one point mutation leading to an amino acids exchange, wherein the mutation is selected from at least one of a group comprising a mutation at position 1308583 and at position 1308589, resulting in exchange from Serine to Proline and/or a mutation at position 1308951 and at position 1309390 resulting in exchange from Phenylalanine to Leucine. a) Gene YDL248W named COS7 located at Chromosome IV, wherein the mutation is a deletion downstream to position 2232; b) Gene YJR155W named AAD10 located at Chromosome X, wherein the mutation is a deletion downstream to position 727404; c) Gene YDL245C named HXT15 located at Chromosome IV, wherein the mutation is a deletion downstream to position 11657; d) Gene YJR151C named DAN4 located at Chromosome X, wherein the mutation is an introduction of at least one stop codon at amino acid position 342 and/or at amino acid position 53 and/or at least partial deletion and/or genomic rearrangement between coordinates of Chromosome X:714,902-715,267; and e) Gene YDR420W named HKR1 located at Chromosome IV, wherein the mutation is at least one point mutation leading to an amino acids exchange, wherein the mutation is selected from at least one of a group comprising a mutation at position 1308583 and at position 1308589, resulting in exchange from Serine to Proline and/or a mutation at position 1308951 and at position 1309390 resulting in exchange from Phenylalanine to Leucine.