An artificial chordae tendineae implantation system, comprising a clamping device, a puncture device, a pushing device, and a detection device, the pushing device comprises a pushing shaft; the clamping device comprises a clamping push rod that receives at least an artificial chorda tendinea, a distal clamp and a proximal clamp for cooperatively clamping a leaflet; the distal clamp is disposed at a distal end of the clamping push rod; the proximal clamp is disposed at a distal end of the pushing shaft; the puncture device and the clamping push rod are movably insertedly mounted within the pushing shaft respectively; characterized in that the detection device comprises at least a probe, the probe is movably insertedly mounted within the pushing shaft; a probe outlet is provided at one of a clamping surface of the proximal clamp and a clamping surface of the distal clamp, and a probe accommodation chamber corresponding to the probe outlet is provided at the other one of the clamping surface of the proximal clamp and the clamping surface of the distal clamp; when the distal clamp closes with the proximal clamp, a distal end of the probe protrudes from the probe outlet and is received in the probe accommodation chamber; wherein the artificial chorda tendinea comprises a flexible chorda tendinea main body, the chorda tendinea main body is sleeved with an anti-slip member, the anti-slip member is provided with a fitting surface that fits the leaflet, the anti-slip member slides in an axial direction of the chorda tendinea main body; and wherein the clamping surface of the distal clamp is provided with an accommodation indentation for receiving the anti-slip member. the pushing device comprises a pushing shaft; the clamping device comprises a clamping push rod that receives at least an artificial chorda tendinea, a distal clamp and a proximal clamp for cooperatively clamping a leaflet; the distal clamp is disposed at a distal end of the clamping push rod; the proximal clamp is disposed at a distal end of the pushing shaft; the puncture device and the clamping push rod are movably insertedly mounted within the pushing shaft respectively; characterized in that the detection device comprises at least a probe, the probe is movably insertedly mounted within the pushing shaft; a probe outlet is provided at one of a clamping surface of the proximal clamp and a clamping surface of the distal clamp, and a probe accommodation chamber corresponding to the probe outlet is provided at the other one of the clamping surface of the proximal clamp and the clamping surface of the distal clamp; when the distal clamp closes with the proximal clamp, a distal end of the probe protrudes from the probe outlet and is received in the probe accommodation chamber; wherein the artificial chorda tendinea comprises a flexible chorda tendinea main body, the chorda tendinea main body is sleeved with an anti-slip member, the anti-slip member is provided with a fitting surface that fits the leaflet, the anti-slip member slides in an axial direction of the chorda tendinea main body; and wherein the clamping surface of the distal clamp is provided with an accommodation indentation for receiving the anti-slip member.