An organic light-emitting display substrate, comprising a display area and a non-display area surrounding the display area, the display area comprising at least one opening penetrating through the organic light-emitting display substrate, the organic light-emitting display substrate comprising: a substrate; an organic layer located on one side of the substrate; a first inorganic layer located on one side of the organic layer away from the substrate, wherein an overall structure of the first inorganic layer and the organic layer has at least one annular partition groove corresponding to each of the at least one opening, the at least one annular partition groove surrounds a periphery of the each of the at least one opening and extends into the organic layer towards the substrate, and a width of an orthographic projection of a notch of each of the at least one annular partition groove on the substrate is smaller than a width of an orthographic projection of the each of the at least one annular partition groove on the substrate; an anode layer located on one side of the first inorganic layer away from the substrate, and comprising a plurality of anodes and an annular covering portion corresponding to each of the at least one annular partition groove, wherein the annular covering portion at least covers a bottom wall and two side walls of an annular partition groove of the at least one annular partition groove corresponding to the annular covering portion, and an organic functional layer located on one side of the anode layer away from the substrate, and comprising a first organic functional material portion located outside the at least one annular partition groove, and a second organic functional material portion located inside the at least one annular partition groove and not connected to the first organic functional material portion and characterized in that a material of the annular covering portion is the same as a material of the plurality of anodes (161.) a substrate; an organic layer located on one side of the substrate; a first inorganic layer located on one side of the organic layer away from the substrate, wherein an overall structure of the first inorganic layer and the organic layer has at least one annular partition groove corresponding to each of the at least one opening, the at least one annular partition groove surrounds a periphery of the each of the at least one opening and extends into the organic layer towards the substrate, and a width of an orthographic projection of a notch of each of the at least one annular partition groove on the substrate is smaller than a width of an orthographic projection of the each of the at least one annular partition groove on the substrate; an anode layer located on one side of the first inorganic layer away from the substrate, and comprising a plurality of anodes and an annular covering portion corresponding to each of the at least one annular partition groove, wherein the annular covering portion at least covers a bottom wall and two side walls of an annular partition groove of the at least one annular partition groove corresponding to the annular covering portion, and an organic functional layer located on one side of the anode layer away from the substrate, and comprising a first organic functional material portion located outside the at least one annular partition groove, and a second organic functional material portion located inside the at least one annular partition groove and not connected to the first organic functional material portion and characterized in that a material of the annular covering portion is the same as a material of the plurality of anodes (161.)