A display substrate, comprising a plurality of pixel groups, wherein each of said pixel groups comprises four color sub-pixels (101, 102, 103, 104) arranged in a 2 × 2 matrix and four white sub-pixels (1010, 1020, 1030, 1040), each color sub-pixel corresponding to one white sub-pixel, each color sub-pixel comprises a light emitting region and a light emitting layer missing region, a first driving transistor is provided in the light emitting layer missing region, for driving the color sub-pixel, a second driving transistor and a white sub-pixel corresponding to the color sub-pixel are provided on the first driving transistor, the second driving transistor for driving the white sub-pixel, characterized in that each of the pixel groups comprises: a first color sub-pixel in the upper left, wherein a light emitting layer missing region of the first color sub-pixel is located in the upper left of the first sub-pixel; a second color sub-pixel in the upper right, wherein a light emitting layer missing region of the second color sub-pixel is located in the upper right of the second sub-pixel; a third color sub-pixel in the lower left, wherein a light emitting layer missing region of the third color sub-pixel is located in the lower right of the third sub-pixel; a fourth color sub-pixel in the lower right, wherein a light emitting layer missing region of the fourth color sub-pixel is located in the lower left of the fourth sub-pixel. wherein each of said pixel groups comprises four color sub-pixels (101, 102, 103, 104) arranged in a 2 × 2 matrix and four white sub-pixels (1010, 1020, 1030, 1040), each color sub-pixel corresponding to one white sub-pixel, each color sub-pixel comprises a light emitting region and a light emitting layer missing region, a first driving transistor is provided in the light emitting layer missing region, for driving the color sub-pixel, a second driving transistor and a white sub-pixel corresponding to the color sub-pixel are provided on the first driving transistor, the second driving transistor for driving the white sub-pixel, characterized in that each of the pixel groups comprises: a first color sub-pixel in the upper left, wherein a light emitting layer missing region of the first color sub-pixel is located in the upper left of the first sub-pixel; a second color sub-pixel in the upper right, wherein a light emitting layer missing region of the second color sub-pixel is located in the upper right of the second sub-pixel; a third color sub-pixel in the lower left, wherein a light emitting layer missing region of the third color sub-pixel is located in the lower right of the third sub-pixel; a fourth color sub-pixel in the lower right, wherein a light emitting layer missing region of the fourth color sub-pixel is located in the lower left of the fourth sub-pixel. a first color sub-pixel in the upper left, wherein a light emitting layer missing region of the first color sub-pixel is located in the upper left of the first sub-pixel; a second color sub-pixel in the upper right, wherein a light emitting layer missing region of the second color sub-pixel is located in the upper right of the second sub-pixel; a third color sub-pixel in the lower left, wherein a light emitting layer missing region of the third color sub-pixel is located in the lower right of the third sub-pixel; a fourth color sub-pixel in the lower right, wherein a light emitting layer missing region of the fourth color sub-pixel is located in the lower left of the fourth sub-pixel.