A bio-cleansing enzymatic kit suitable for removal pre-existing biological and chemical entities from a substrate, comprising: - an enzyme system comprising a plurality of immobilized enzymes, linked or associated in a stable or temporary manner, to one or more particles; - a formulation comprising one or more of said enzyme systems, characterized in that: - said kit further comprises means where said formulation is applied thereto, or means impregnated with said formulation, or a medium wherein said enzyme system is dispersed, said medium being included in said formulation; - said particles are inorganic particles; - said formulation can be removed with ease from said substrate to preserve substrate integrity; and in that: - said particles have a substantially spherical shape with a diameter in the range between about 100 to about 300 nm and have mesoporous pore diameter in the range between 2.0 to 2.5 nm; - said enzymatic system contains trypsin, in an amount between 2.5 to 20 mg/g; - said medium is a methylcellulose gel or a hydrophilic fabric; - said formulation can be removed with ease from said substrate using water or water-based solutions; and wherein said bio-cleansing enzymatic kit comprises a buffer solution wherein said enzyme system is dispersed or that wets said hydrophilic fabric, said buffer solution being selected from the group consisting of: phosphate buffer solution, sodium citrate buffer solution, or a combination thereof. - an enzyme system comprising a plurality of immobilized enzymes, linked or associated in a stable or temporary manner, to one or more particles; - a formulation comprising one or more of said enzyme systems, characterized in that: - said kit further comprises means where said formulation is applied thereto, or means impregnated with said formulation, or a medium wherein said enzyme system is dispersed, said medium being included in said formulation; - said particles are inorganic particles; - said formulation can be removed with ease from said substrate to preserve substrate integrity; and in that: - said particles have a substantially spherical shape with a diameter in the range between about 100 to about 300 nm and have mesoporous pore diameter in the range between 2.0 to 2.5 nm; - said enzymatic system contains trypsin, in an amount between 2.5 to 20 mg/g; - said medium is a methylcellulose gel or a hydrophilic fabric; - said formulation can be removed with ease from said substrate using water or water-based solutions; - said kit further comprises means where said formulation is applied thereto, or means impregnated with said formulation, or a medium wherein said enzyme system is dispersed, said medium being included in said formulation; - said particles are inorganic particles; - said formulation can be removed with ease from said substrate to preserve substrate integrity; and in that: - said particles have a substantially spherical shape with a diameter in the range between about 100 to about 300 nm and have mesoporous pore diameter in the range between 2.0 to 2.5 nm; - said enzymatic system contains trypsin, in an amount between 2.5 to 20 mg/g; - said medium is a methylcellulose gel or a hydrophilic fabric; - said formulation can be removed with ease from said substrate using water or water-based solutions; - said particles have a substantially spherical shape with a diameter in the range between about 100 to about 300 nm and have mesoporous pore diameter in the range between 2.0 to 2.5 nm; - said enzymatic system contains trypsin, in an amount between 2.5 to 20 mg/g; - said medium is a methylcellulose gel or a hydrophilic fabric; - said formulation can be removed with ease from said substrate using water or water-based solutions;