A coherent anti-Stokes Raman scattering microscope imaging device, comprising: a laser light source, a two-dimensional galvanometer assembly, a first dichroic mirror, an objective lens, a sample translation platform, a collector, and a data processing module; wherein the laser light source is configured to generate a first laser beam and a second laser beam; the first laser beam and the second laser beam are output collinearly; the first laser beam and the second laser beam are incident on the two-dimensional galvanometer assembly, the two-dimensional galvanometer assembly adjusts optical paths of the first laser beam and the second laser beam; the first laser beam and the second laser beam leaving the two-dimensional galvanometer assembly pass through the first dichroic mirror and the objective lens successively; the objective lens focuses the first laser beam and the second laser beam onto the sample translation platform; a signal light generated by the sample translation platform passes through the objective lens, the first dichroic mirror reflects the signal light to the collector, and the collector generates initial data according to the signal light, and outputs the initial data to the data processing module; characterized in that the coherent anti-Stokes Raman scattering microscope imaging device further comprises an automatic focusing mechanism, wherein the automatic focusing mechanism comprises a focus detection unit, a second dichroic mirror, and a moving assembly; the second dichroic mirror is arranged in correspondence with the first dichroic mirror and the objective lens, respectively; the first laser beam and the second laser beam are reflected to the objective lens through the second dichroic mirror after passing through the first dichroic mirror; the signal light is reflected to the first dichroic mirror through the second dichroic mirror after passing through the objective lens; the focus detection unit generates a third laser beam that transmits through the second dichroic mirror; the third laser beam is focused on the sample translation platform through the objective lens, and the third laser beam generates a reflected light on the sample translation platform; the reflected light returns to the focus detection unit along an original path of the third laser beam; the focus detection unit detects the reflected light; the objective lens is mounted on the moving assembly; the moving assembly moves the objective lens according to a detection result of the focus detection unit; wherein the second dichroic mirror is a short-wave dichroic mirror, and the surface of the second dichroic mirror is plated with a dielectric film to achieve reflection of the first laser beam, the second laser beam and the signal light, while realizing the passage of the third laser beam and the reflected light. the coherent anti-Stokes Raman scattering microscope imaging device further comprises an automatic focusing mechanism, wherein the automatic focusing mechanism comprises a focus detection unit, a second dichroic mirror, and a moving assembly; the second dichroic mirror is arranged in correspondence with the first dichroic mirror and the objective lens, respectively; the first laser beam and the second laser beam are reflected to the objective lens through the second dichroic mirror after passing through the first dichroic mirror; the signal light is reflected to the first dichroic mirror through the second dichroic mirror after passing through the objective lens; the focus detection unit generates a third laser beam that transmits through the second dichroic mirror; the third laser beam is focused on the sample translation platform through the objective lens, and the third laser beam generates a reflected light on the sample translation platform; the reflected light returns to the focus detection unit along an original path of the third laser beam; the focus detection unit detects the reflected light; the objective lens is mounted on the moving assembly; the moving assembly moves the objective lens according to a detection result of the focus detection unit; wherein the second dichroic mirror is a short-wave dichroic mirror, and the surface of the second dichroic mirror is plated with a dielectric film to achieve reflection of the first laser beam, the second laser beam and the signal light, while realizing the passage of the third laser beam and the reflected light.