A method for alignment of an ultrasound image obtained by an ultrasound probe for obtaining images in relation to the eye, the method comprising: placing the ultrasound probe in a suitable location for obtaining images of anatomical structures in the region of the eye and across a region of interest expected to include the optic nerve; and obtaining images of said region of interest using the ultrasound probe; characterised by: processing the images of said region of interest via computer implemented image processing techniques in order to identify boundary features (4, 5, 6) in the images that are representative of the boundaries of at least one of the optic nerve and the optic nerve sheath; using the identified boundary features of the imaged structure to determine a principal direction extending along the length of the optic nerve and the optic nerve sheath; identifying at least first and second points on the identified boundary features, wherein the first and second points are at different locations along the principal direction; and rotating the image plane of the probe until the first and second points are aligned in the image, and thereby determining a required orientation of the image plane of the probe for alignment of the ultrasound image with the principal direction. placing the ultrasound probe in a suitable location for obtaining images of anatomical structures in the region of the eye and across a region of interest expected to include the optic nerve; and obtaining images of said region of interest using the ultrasound probe; characterised by: processing the images of said region of interest via computer implemented image processing techniques in order to identify boundary features (4, 5, 6) in the images that are representative of the boundaries of at least one of the optic nerve and the optic nerve sheath; using the identified boundary features of the imaged structure to determine a principal direction extending along the length of the optic nerve and the optic nerve sheath; identifying at least first and second points on the identified boundary features, wherein the first and second points are at different locations along the principal direction; and rotating the image plane of the probe until the first and second points are aligned in the image, and thereby determining a required orientation of the image plane of the probe for alignment of the ultrasound image with the principal direction. processing the images of said region of interest via computer implemented image processing techniques in order to identify boundary features (4, 5, 6) in the images that are representative of the boundaries of at least one of the optic nerve and the optic nerve sheath; using the identified boundary features of the imaged structure to determine a principal direction extending along the length of the optic nerve and the optic nerve sheath; identifying at least first and second points on the identified boundary features, wherein the first and second points are at different locations along the principal direction; and rotating the image plane of the probe until the first and second points are aligned in the image, and thereby determining a required orientation of the image plane of the probe for alignment of the ultrasound image with the principal direction.