An insulation detection circuit, comprising: a first voltage dividing module, wherein, one end of the first voltage dividing module is connected to a positive electrode of a power battery to be detected, and the other end of the first voltage dividing module is connected to a first isolation module and a second voltage dividing module respectively; the second voltage dividing module, connected to a negative electrode of the power battery to be detected; the first isolation module, connected to one end of a sampling module; the sampling module, wherein, the other end of the sampling module is connected to one end of a signal generating module; the signal generating module, wherein, the other end of the signal generating module is connected to power ground, and the signal generating module is configured to inject a signal at a predetermined frequency to the power battery to be detected; and a processing module, configured to calculate an insulation resistance value of the power battery to be detected according to a first sampled signal collected at the one end of the sampling module and a second sampled signal collected at the one end of the signal generating module, wherein the insulation detection circuit is characterized in that: the first voltage dividing module comprises a first resistor, one end of the first resistor is connected to the positive electrode, and the other end of the first resistor is connected to the first isolation module and the second voltage dividing module respectively; the second voltage dividing module comprises: a second resistor, wherein, one end of the second resistor is connected to the other end of the first resistor, and the other end of the second resistor is connected to a first switch; and the first switch, connected to the negative electrode; a resistance value of the first resistor is equal to a resistance value of the second resistor; and the first isolation module comprises: a second switch, connected to the other end of the first voltage dividing module; and an isolation capacitor, connected to the second switch and the sampling module respectively. a first voltage dividing module, wherein, one end of the first voltage dividing module is connected to a positive electrode of a power battery to be detected, and the other end of the first voltage dividing module is connected to a first isolation module and a second voltage dividing module respectively; the second voltage dividing module, connected to a negative electrode of the power battery to be detected; the first isolation module, connected to one end of a sampling module; the sampling module, wherein, the other end of the sampling module is connected to one end of a signal generating module; the signal generating module, wherein, the other end of the signal generating module is connected to power ground, and the signal generating module is configured to inject a signal at a predetermined frequency to the power battery to be detected; and a processing module, configured to calculate an insulation resistance value of the power battery to be detected according to a first sampled signal collected at the one end of the sampling module and a second sampled signal collected at the one end of the signal generating module, wherein the insulation detection circuit is characterized in that: the first voltage dividing module comprises a first resistor, one end of the first resistor is connected to the positive electrode, and the other end of the first resistor is connected to the first isolation module and the second voltage dividing module respectively; the second voltage dividing module comprises: a second resistor, wherein, one end of the second resistor is connected to the other end of the first resistor, and the other end of the second resistor is connected to a first switch; and the first switch, connected to the negative electrode; a resistance value of the first resistor is equal to a resistance value of the second resistor; and the first isolation module comprises: a second switch, connected to the other end of the first voltage dividing module; and an isolation capacitor, connected to the second switch and the sampling module respectively. the first voltage dividing module comprises a first resistor, one end of the first resistor is connected to the positive electrode, and the other end of the first resistor is connected to the first isolation module and the second voltage dividing module respectively; the second voltage dividing module comprises: a second resistor, wherein, one end of the second resistor is connected to the other end of the first resistor, and the other end of the second resistor is connected to a first switch; and the first switch, connected to the negative electrode; a resistance value of the first resistor is equal to a resistance value of the second resistor; and the first isolation module comprises: a second switch, connected to the other end of the first voltage dividing module; and an isolation capacitor, connected to the second switch and the sampling module respectively.