A method for the quantification of an influenza hemagglutinin target protein present in a sample comprising the steps of: (a) immobilizing at least three capture agents and an internal reference control onto a provided array, said provided array comprising a solid support onto which said at least three capture agents are affixed in a plurality of discrete areas, said plurality of discrete areas only containing a single unique capture agent and arranged in a configuration of decreasing concentration of each of said at least three capture agents, wherein said capture agents allow the quantification of HA antigens from influenza A H1N1, influenza A H3N2 and influenza B; (b) contacting (i) at least three replicates of said array with a reference material comprising influenza hemagglutinin so as to allow binding between said reference material and said at least three capture agents, each replicate of said array being contacted with a different concentration of said reference material and (ii) a replicate of said array with at least one target material comprising influenza hemagglutinin so as to allow binding between said at least one target material and said at least three capture agents; (c) contacting said arrays with at least one label agent to produce a detectable signal indicative of said binding between reference or target material(s) and capture agents; (d) processing said detectable signals of said arrays using a ratio of said detectable signals to detectable signals from said internal reference control to yield a normalized signal; (e) analyzing said normalized signals of said arrays contacted with said reference material or target material as a function of the capture agent concentration immobilized on the arrays so as to determine the maximum signal response (S max) achieved on each array for each concentration of reference or the target material by non-linear regression; (f) constructing a concentration calibration curve comprising said maximum signal responses (S max) as a function of the concentration of said reference material applied to said arrays; and (g) quantifying said target material using said calibration curve. (a) immobilizing at least three capture agents and an internal reference control onto a provided array, said provided array comprising a solid support onto which said at least three capture agents are affixed in a plurality of discrete areas, said plurality of discrete areas only containing a single unique capture agent and arranged in a configuration of decreasing concentration of each of said at least three capture agents, wherein said capture agents allow the quantification of HA antigens from influenza A H1N1, influenza A H3N2 and influenza B; (b) contacting (i) at least three replicates of said array with a reference material comprising influenza hemagglutinin so as to allow binding between said reference material and said at least three capture agents, each replicate of said array being contacted with a different concentration of said reference material and (ii) a replicate of said array with at least one target material comprising influenza hemagglutinin so as to allow binding between said at least one target material and said at least three capture agents; (c) contacting said arrays with at least one label agent to produce a detectable signal indicative of said binding between reference or target material(s) and capture agents; (d) processing said detectable signals of said arrays using a ratio of said detectable signals to detectable signals from said internal reference control to yield a normalized signal; (e) analyzing said normalized signals of said arrays contacted with said reference material or target material as a function of the capture agent concentration immobilized on the arrays so as to determine the maximum signal response (S max) achieved on each array for each concentration of reference or the target material by non-linear regression; (f) constructing a concentration calibration curve comprising said maximum signal responses (S max) as a function of the concentration of said reference material applied to said arrays; and (g) quantifying said target material using said calibration curve.