A thermal cycler device, comprising: an annular conveying element, having a closed circular conveying path; a plurality of slide plate device holding elements, disposed on the annular conveying element configured for holding a plurality of slide plate devices, and each of the slide plate device holding elements are regularly arranged side by side along the circular conveying path at the same angle; a plurality of heating blocks, disposed under the annular conveying element; and a cooling device, configured to cool the plurality of the heating blocks, wherein the thermal cycler device is configured so that the annular conveying element is operated in stages, such that the plurality of the slide plate device holding elements carry the plurality of slide plate devices to move along the circular conveying path, when each of the slide plate device holding elements moves to the respective corresponding heating block, the thermal cycler device being characterized in further comprising: a pressing element, disposed above the plurality of slide plate device holding elements, having a plurality of pressing blocks, each of the pressing blocks respectively corresponding to each of the heating blocks; and in that the annular conveying element is configured to stop operating, and each of the pressing blocks is configured to perform a pressing process to make each of the slide plate devices to be in contact with the corresponding heating block for heat transfer. an annular conveying element, having a closed circular conveying path; a plurality of slide plate device holding elements, disposed on the annular conveying element configured for holding a plurality of slide plate devices, and each of the slide plate device holding elements are regularly arranged side by side along the circular conveying path at the same angle; a plurality of heating blocks, disposed under the annular conveying element; and a cooling device, configured to cool the plurality of the heating blocks, wherein the thermal cycler device is configured so that the annular conveying element is operated in stages, such that the plurality of the slide plate device holding elements carry the plurality of slide plate devices to move along the circular conveying path, when each of the slide plate device holding elements moves to the respective corresponding heating block, the thermal cycler device being characterized in further comprising: a pressing element, disposed above the plurality of slide plate device holding elements, having a plurality of pressing blocks, each of the pressing blocks respectively corresponding to each of the heating blocks; and in that the annular conveying element is configured to stop operating, and each of the pressing blocks is configured to perform a pressing process to make each of the slide plate devices to be in contact with the corresponding heating block for heat transfer. a pressing element, disposed above the plurality of slide plate device holding elements, having a plurality of pressing blocks, each of the pressing blocks respectively corresponding to each of the heating blocks; and in that the annular conveying element is configured to stop operating, and each of the pressing blocks is configured to perform a pressing process to make each of the slide plate devices to be in contact with the corresponding heating block for heat transfer.