A protection circuit of a communication interface, comprising: a first-level protection circuit, a second-level protection circuit and a third-level protection circuit that are sequentially connected, characterized in that, the first-level protection circuit comprises a current discharge circuit, the current discharge circuit comprising a first connection end, a second connection end and a third connection end, wherein the first connection end is connected with a first signal line of an RS-485 port, the second connection end is connected with a second signal line of the RS-485 port, and the third connection end is connected to a protection ground, and the third connection end introduces generated current to the ground when the RS-485 port is intruded by a voltage; the second-level protection circuit comprises a first current limiting resistor, a second current limiting resistor and a bidirectional transient voltage suppressor, wherein, the first current limiting resistor is connected with the first signal line, the second current limiting resistor is connected with the second signal line, and the two ends of the bidirectional transient voltage suppressor are connected respectively with the first signal line which is connected with the first current limiting resistor and the second signal line which is connected with the second current limiting resistor; and the third-level protection circuit comprises a voltage discharge circuit, wherein the voltage discharge circuit is connected to a communication ground, and one end of the voltage discharge circuit is connected with the two signal lines of the RS-485 port, and the other end of the voltage discharge circuit is connected with a RS-485 transceiver, the voltage discharge circuit is connected to the communication ground which is electrically isolated from the protection ground, and provides a voltage discharge path for the voltage between the second-level protection circuit and the RS-485 transceiver; wherein, the voltage discharge circuit comprises any of the following: a first bi-end bidirectional transient voltage suppressor and a second bi-end bidirectional transient voltage suppressor, wherein one end of the first bi-end bidirectional transient voltage suppressor is connected with the first signal line, and the other end of the first bi-end bidirectional transient voltage suppressor is connected to the communication ground; and one end of the second bi-end bidirectional transient voltage suppressor is connected with the second signal line, and the other end of the bi-end bidirectional transient voltage suppressor is connected to the communication ground; and a tri-end double uni-directional transient voltage suppressor and a bi-end uni-directional transient voltage suppressor, wherein, a first non-common end out of two non-common ends of the tri-end double uni-directional transient voltage suppressor is connected with the first signal line, and a second non-common end out of the two non-common ends of the tri-end double uni-directional transient voltage suppressor is connected with the second signal line; and one end of the bi-end uni-directional transient voltage suppressor is connected to a common end of the tri-end double uni-directional transient voltage suppressor, and the other end of the bi-end uni-directional transient voltage suppressor is connected to the communication ground. a first bi-end bidirectional transient voltage suppressor and a second bi-end bidirectional transient voltage suppressor, wherein one end of the first bi-end bidirectional transient voltage suppressor is connected with the first signal line, and the other end of the first bi-end bidirectional transient voltage suppressor is connected to the communication ground; and one end of the second bi-end bidirectional transient voltage suppressor is connected with the second signal line, and the other end of the bi-end bidirectional transient voltage suppressor is connected to the communication ground; and a tri-end double uni-directional transient voltage suppressor and a bi-end uni-directional transient voltage suppressor, wherein, a first non-common end out of two non-common ends of the tri-end double uni-directional transient voltage suppressor is connected with the first signal line, and a second non-common end out of the two non-common ends of the tri-end double uni-directional transient voltage suppressor is connected with the second signal line; and one end of the bi-end uni-directional transient voltage suppressor is connected to a common end of the tri-end double uni-directional transient voltage suppressor, and the other end of the bi-end uni-directional transient voltage suppressor is connected to the communication ground.