A subtilisin, recombinant polypeptide or active fragment thereof, wherein said subtilisin, polypeptide or fragment comprises one or more motifs selected from: (i) DTGIXXXHXDLXXXXXGGXSVFTDSXXXXXXXDXXGH (SEQ ID NO: 11) motif, wherein the initial D is the active site Aspartic acid, the terminal H is the active site Histidine, and X is any amino acid; (ii) DTGIXXXHXDLX a XXXXGGXSVFTDSXXXXXXXDXXGH (SEQ ID NO: 12) motif, wherein the initial D is the active site Aspartic acid, the terminal H is the active site Histidine, X is any amino acid, and X a is T, S or For X a is S or F; (iii) DTGIXXXHXDLXXXXXGGXSVFTDSXXX b XXXXDXXGH (SEQ ID NO: 13) motif, wherein the initial D is the active site Aspartic acid, the terminal H is the active site Histidine, X is any amino acid, and X b is S or R, or X b is S; (iv) DTGIXXXHXDLX a XXXXGGXSVFTDSXXX b XXXXDXXGH (SEQ ID NO: 14) motif, wherein the initial D is the active site Aspartic acid; the terminal H is the active site Histidine; X is any amino acid; X a is T, S or F or X a is S or F; and X b is S or R or X b is S; (v) DTGIXXXHXDLXANVXGGXSVFTDSANXDPFXDXXGH (SEQ ID NO: 15) motif, wherein the initial D is the active site Aspartic acid and the terminal H is the active site Histidine, and X is any amino acid; (vi) HXDLXANVXGGXS (SEQ ID NO: 16) motif, wherein the initial D is the active site Aspartic acid, the terminal GGXS is in the outermost strand of the central beta sheet, and X is any amino acid; (vii) GGXSVFTDSANXDPFXD (SEQ ID NO: 17) motif, wherein the initial GGXS is in the outermost strand of the central beta sheet and X is any amino acid; (viii) HXDLX a ANVXGGXS (SEQ ID NO: 18) motif, wherein the initial D is the active site Aspartic acid; the terminal GGXS is in the outermost strand of the central beta sheet; X a is T, S or F or X a is S or F; and X is any amino acid; (ix) GGXSVFTDSANX b DPFXD (SEQ ID NO: 19) motif, wherein the initial GGXS is in the outermost strand of the central beta sheet, X is any amino acid, and X b is S or R, or X b is S, and wherein the amino acid sequence of said subtilisin, polypeptide or fragment has at least 95% amino acid sequence identity to the amino acid sequence of SEQ ID NO: 9, and wherein said subtilisin, polypeptide or fragment has protease activity in the presence of a surfactant. (i) DTGIXXXHXDLXXXXXGGXSVFTDSXXXXXXXDXXGH (SEQ ID NO: 11) motif, wherein the initial D is the active site Aspartic acid, the terminal H is the active site Histidine, and X is any amino acid; (ii) DTGIXXXHXDLX a XXXXGGXSVFTDSXXXXXXXDXXGH (SEQ ID NO: 12) motif, wherein the initial D is the active site Aspartic acid, the terminal H is the active site Histidine, X is any amino acid, and X a is T, S or For X a is S or F; (iii) DTGIXXXHXDLXXXXXGGXSVFTDSXXX b XXXXDXXGH (SEQ ID NO: 13) motif, wherein the initial D is the active site Aspartic acid, the terminal H is the active site Histidine, X is any amino acid, and X b is S or R, or X b is S; (iv) DTGIXXXHXDLX a XXXXGGXSVFTDSXXX b XXXXDXXGH (SEQ ID NO: 14) motif, wherein the initial D is the active site Aspartic acid; the terminal H is the active site Histidine; X is any amino acid; X a is T, S or F or X a is S or F; and X b is S or R or X b is S; (v) DTGIXXXHXDLXANVXGGXSVFTDSANXDPFXDXXGH (SEQ ID NO: 15) motif, wherein the initial D is the active site Aspartic acid and the terminal H is the active site Histidine, and X is any amino acid; (vi) HXDLXANVXGGXS (SEQ ID NO: 16) motif, wherein the initial D is the active site Aspartic acid, the terminal GGXS is in the outermost strand of the central beta sheet, and X is any amino acid; (vii) GGXSVFTDSANXDPFXD (SEQ ID NO: 17) motif, wherein the initial GGXS is in the outermost strand of the central beta sheet and X is any amino acid; (viii) HXDLX a ANVXGGXS (SEQ ID NO: 18) motif, wherein the initial D is the active site Aspartic acid; the terminal GGXS is in the outermost strand of the central beta sheet; X a is T, S or F or X a is S or F; and X is any amino acid; (ix) GGXSVFTDSANX b DPFXD (SEQ ID NO: 19) motif, wherein the initial GGXS is in the outermost strand of the central beta sheet, X is any amino acid, and X b is S or R, or X b is S, and wherein the amino acid sequence of said subtilisin, polypeptide or fragment has at least 95% amino acid sequence identity to the amino acid sequence of SEQ ID NO: 9, and wherein said subtilisin, polypeptide or fragment has protease activity in the presence of a surfactant.