mhurhangee/ep-ind-claims huggingface dataset:
A method for producing a composition comprising an RNA transcript for a gene of interest, the method comprising: (i) obtaining a sample comprising a linear, non-amplified DNA template, the DNA template comprising an RNA polymerase promoter sequence operatively linked to a target sequence coding for the gene of interest a poly A tail sequence of 60-160 nucleotides, an endonuclease recognition site sequence immediately downstream of the poly A tail sequence, optionally a 5' untranslated region and/or a 3' UTR, wherein the DNA template is produced at a milligram scale as a circular plasmid and linearized by a method consisting of contacting the DNA template with an endonuclease that recognizes the endonuclease recognition site sequence; (ii) contacting the sample with a T7 RNA polymerase and ribonucleotides to form a first reaction under conditions sufficient to produce the composition comprising the RNA transcript wherein at least 80% of the RNA transcript is full-length RNA transcript; (iii) purifying the RNA transcript using oligo dT affinity purification; (iv) capping the RNA transcript by contacting the RNA transcript with guanylyltransferase, s-adenosyl-L-methionine; guanosine triphosphate, and optionally 2'-O-methyltransferase in a second reaction; (v) purifying the RNA transcript using an anion exchange chromatography step; and (vi) filtering the RNA transcript using an ultrafiltration or tangential flow filtration membrane, thereby producing the composition comprising the RNA transcript. (i) obtaining a sample comprising a linear, non-amplified DNA template, the DNA template comprising an RNA polymerase promoter sequence operatively linked to a target sequence coding for the gene of interest a poly A tail sequence of 60-160 nucleotides, an endonuclease recognition site sequence immediately downstream of the poly A tail sequence, optionally a 5' untranslated region and/or a 3' UTR, wherein the DNA template is produced at a milligram scale as a circular plasmid and linearized by a method consisting of contacting the DNA template with an endonuclease that recognizes the endonuclease recognition site sequence; (ii) contacting the sample with a T7 RNA polymerase and ribonucleotides to form a first reaction under conditions sufficient to produce the composition comprising the RNA transcript wherein at least 80% of the RNA transcript is full-length RNA transcript; (iii) purifying the RNA transcript using oligo dT affinity purification; (iv) capping the RNA transcript by contacting the RNA transcript with guanylyltransferase, s-adenosyl-L-methionine; guanosine triphosphate, and optionally 2'-O-methyltransferase in a second reaction; (v) purifying the RNA transcript using an anion exchange chromatography step; and (vi) filtering the RNA transcript using an ultrafiltration or tangential flow filtration membrane,
A consumer product comprising a liquid laundry treatment composition housed in a container, wherein; a. the container comprises a spray applicator and an internal chamber comprising the laundry treatment composition and the liquid laundry treatment composition is in contact with the spray applicator and the spray applicator is capable of spraying the liquid laundry treatment composition into the environment external to the container upon activation of the spray applicator; b. the liquid laundry treatment composition comprises at least 80% by weight of the liquid laundry detergent composition of water; and between 5% and 10% by weight of the liquid laundry treatment composition of non-soap surfactant, wherein the non-soap surfactant comprises non-soap anionic surfactant and the non-soap surfactant comprises 100% by weight of the non-soap surfactant of the non-soap anionic surfactant, wherein the liquid laundry treatment composition comprises from 0.3% to 0.7% by weight of the liquid laundry treatment composition of a cationic polymer wherein the cationic polymer is a hydroxyethyl cellulose derivatised with trimethyl ammonium substituted epoxide, and wherein the non-soap anionic surfactant comprises a sulphate or a sulphonate anionic surfactant or a mixture thereof. a. the container comprises a spray applicator and an internal chamber comprising the laundry treatment composition and the liquid laundry treatment composition is in contact with the spray applicator and the spray applicator is capable of spraying the liquid laundry treatment composition into the environment external to the container upon activation of the spray applicator; b. the liquid laundry treatment composition comprises at least 80% by weight of the liquid laundry detergent composition of water; and between 5% and 10% by weight of the liquid laundry treatment composition of non-soap surfactant, wherein the non-soap surfactant comprises non-soap anionic surfactant and the non-soap surfactant comprises 100% by weight of the non-soap surfactant of the non-soap anionic surfactant, wherein the liquid laundry treatment composition comprises from 0.3% to 0.7% by weight of the liquid laundry treatment composition of a cationic polymer wherein the cationic polymer is a hydroxyethyl cellulose derivatised with trimethyl ammonium substituted epoxide, and wherein the non-soap anionic surfactant comprises a sulphate or a sulphonate anionic surfactant or a mixture thereof. wherein the liquid laundry treatment composition comprises from 0.3% to 0.7% by weight of the liquid laundry treatment composition of a cationic polymer wherein the cationic polymer is a hydroxyethyl cellulose derivatised with trimethyl ammonium substituted epoxide, and wherein the non-soap anionic surfactant comprises a sulphate or a sulphonate anionic surfactant or a mixture thereof.
An aqueous composition comprising an aryl phosphate surfactant and a multistage polymer, wherein the multistage polymer comprises (i) a first stage comprising a first copolymer having a first theoretical glass transition temperature (T g), the first copolymer being derived from a soft ethylenically-unsaturated monomer, a phosphorus-containing monomer, and an acetoacetoxy monomer; and (ii) a second stage comprising a second polymer having a second theoretical T g, the second polymer being derived from one or more hard ethylenically-unsaturated monomers. (i) a first stage comprising a first copolymer having a first theoretical glass transition temperature (T g), the first copolymer being derived from a soft ethylenically-unsaturated monomer, a phosphorus-containing monomer, and an acetoacetoxy monomer; and (ii) a second stage comprising a second polymer having a second theoretical T g, the second polymer being derived from one or more hard ethylenically-unsaturated monomers.
A computer-executable method for facilitating network service virtualization in a content-centric network, comprising: receiving by a proxy server (114, 120) a service request from a client; inspecting the service request for session state information that can identify the client and a previous communication session; initializing a communication session with the client; constructing, by the proxy server, an interest packet, wherein the interest packet comprises a hierarchically structured variable-length name and wherein the hierarchically structured variable length name indicates the session state information; broadcasting the interest packet to a plurality of servers (108, 110, 112) to cause each of the plurality of servers to compare the session state information with session state information stored at the respective server and to cause at least a first server storing session state information that matches the session state information indicated in the heirarchically structured variable length name to respond first to the proxy server in response to the interest packet; receiving data from the first server of the plurality of servers; selecting the first server to establish a session between the client and the first server; and forwarding to the client the data received from the first server. receiving by a proxy server (114, 120) a service request from a client; inspecting the service request for session state information that can identify the client and a previous communication session; initializing a communication session with the client; constructing, by the proxy server, an interest packet, wherein the interest packet comprises a hierarchically structured variable-length name and wherein the hierarchically structured variable length name indicates the session state information; broadcasting the interest packet to a plurality of servers (108, 110, 112) to cause each of the plurality of servers to compare the session state information with session state information stored at the respective server and to cause at least a first server storing session state information that matches the session state information indicated in the heirarchically structured variable length name to respond first to the proxy server in response to the interest packet; receiving data from the first server of the plurality of servers; selecting the first server to establish a session between the client and the first server; and forwarding to the client the data received from the first server.
A method performed at a secondary network node and a master network node within a wireless communication network supporting dual connectivity, such that a user equipment may communicate with a master network node and a secondary network node, said secondary network node supporting a plurality of cells, said method comprising: determining at said secondary network node that a configuration of one of said plurality of cells as a special cell for said user equipment such that said special cell provides predetermined functionality should be transferred to another one of said plurality of cells; and the method being further characterised by, in response to said determining step, transmitting a request for a change in special cell towards said master network node; and receiving from said master network node one of a secondary network node modification or a secondary network node addition request; wherein said master network node is configured to receive radio resource configuration information indicating a change in a cell designated as said special cell at said secondary network node prior to transmitting a radio resource connection reconfiguration request to said user equipment; wherein said radio resource connection reconfiguration request comprises a secondary network node modification request for modifying parameters relating to said secondary network node, said modification request comprising parameters identifying a new special cell; and wherein the special cell is configured to control downlink random access request transmission for Physical Random Access Channel procedure. determining at said secondary network node that a configuration of one of said plurality of cells as a special cell for said user equipment such that said special cell provides predetermined functionality should be transferred to another one of said plurality of cells; and the method being further characterised by, in response to said determining step, transmitting a request for a change in special cell towards said master network node; and receiving from said master network node one of a secondary network node modification or a secondary network node addition request; wherein said master network node is configured to receive radio resource configuration information indicating a change in a cell designated as said special cell at said secondary network node prior to transmitting a radio resource connection reconfiguration request to said user equipment; wherein said radio resource connection reconfiguration request comprises a secondary network node modification request for modifying parameters relating to said secondary network node, said modification request comprising parameters identifying a new special cell; and wherein the special cell is configured to control downlink random access request transmission for Physical Random Access Channel procedure.
An object positioning method comprising: obtaining a reference position of an object; obtaining a map-based heading angle of the object based on waypoints (304, 305) on a map (504; 701); obtaining, using at least one sensor which preferably comprises an acceleration sensor and a direction sensor, a speed and a sensor-based heading angle of the object; generating a second heading angle, comprising: comparing the map-based heading angle and the sensor-based heading angle to determine a difference between the map-based heading angle and the sensor-based heading angle; and when the difference between the map-based heading angle (202; 303; 402; 704; 811) and the sensor-based heading angle (203; 302; 403) is less than a threshold angle, the second heading angle is the map-based heading angle; and when the difference between the map-based heading angle and the sensor-based heading angle is greater than the threshold angle, the second heading angle is the sensor-based heading angle; performing dead reckoning based on the reference position (201; 705; 803), second heading angle, and the speed; and determining a current position of the object based on a result of the dead reckoning. obtaining a reference position of an object; obtaining a map-based heading angle of the object based on waypoints (304, 305) on a map (504; 701); obtaining, using at least one sensor which preferably comprises an acceleration sensor and a direction sensor, a speed and a sensor-based heading angle of the object; generating a second heading angle, comprising: comparing the map-based heading angle and the sensor-based heading angle to determine a difference between the map-based heading angle and the sensor-based heading angle; and when the difference between the map-based heading angle (202; 303; 402; 704; 811) and the sensor-based heading angle (203; 302; 403) is less than a threshold angle, the second heading angle is the map-based heading angle; and when the difference between the map-based heading angle and the sensor-based heading angle is greater than the threshold angle, the second heading angle is the sensor-based heading angle; comparing the map-based heading angle and the sensor-based heading angle to determine a difference between the map-based heading angle and the sensor-based heading angle; and when the difference between the map-based heading angle (202; 303; 402; 704; 811) and the sensor-based heading angle (203; 302; 403) is less than a threshold angle, the second heading angle is the map-based heading angle; and when the difference between the map-based heading angle and the sensor-based heading angle is greater than the threshold angle, the second heading angle is the sensor-based heading angle; performing dead reckoning based on the reference position (201; 705; 803), second heading angle, and the speed; and determining a current position of the object based on a result of the dead reckoning.
A lidar receiver circuit for receiving optical signals by means of photodetectors, in order to detect events or objects in the surrounding area of the lidar receiver circuit, with a redundancy of receiver circuits for reducing the failure probability, comprising a photodetector array with a plurality of photodetectors for receiving optical signals and for outputting a measurement signal, wherein the photodetectors are arranged as a two-dimensional matrix in the form of rows and n columns, with the number of n larger than 1; a plurality of i receiver circuits for receiving the measurement signal of the photodetectors, with the number i larger than n; a plurality of m multiplexers, which are electrically arranged between the photodetector array and the receiver circuits and which are connected to photodetectors and receiver circuits, with the number of m larger than 1; wherein at least one of the multiplexers is connected to a column of photodetectors and to at least two receiver circuits; each receiver circuit is configured to receive and to process the measurement signals of the photodetectors of a column; a column of photodetectors is assigned to each receiver circuit by default; and the at least one multiplexer is configured to connect a column of photodetectors to a receiver circuit other than the receiver circuit, which is assigned to the column of photodetectors by default; wherein the assignment takes place when a defect in one of the receiver circuits of the associated multiplexer is detected. a photodetector array with a plurality of photodetectors for receiving optical signals and for outputting a measurement signal, wherein the photodetectors are arranged as a two-dimensional matrix in the form of rows and n columns, with the number of n larger than 1; a plurality of i receiver circuits for receiving the measurement signal of the photodetectors, with the number i larger than n; a plurality of m multiplexers, which are electrically arranged between the photodetector array and the receiver circuits and which are connected to photodetectors and receiver circuits, with the number of m larger than 1; wherein at least one of the multiplexers is connected to a column of photodetectors and to at least two receiver circuits; each receiver circuit is configured to receive and to process the measurement signals of the photodetectors of a column; a column of photodetectors is assigned to each receiver circuit by default; and the at least one multiplexer is configured to connect a column of photodetectors to a receiver circuit other than the receiver circuit, which is assigned to the column of photodetectors by default; wherein the assignment takes place when a defect in one of the receiver circuits of the associated multiplexer is detected.
A method of manufacturing a gear, comprising: providing a rim gear, a hub and a core, wherein the core is annular and has a core forging temperature below a hot hardness temperature of the rim gear and the hub; rotating, about an axis, the rim gear and the hub relative to the core, the rim gear and the hub in contact with the core to generate friction heat to raise an interface temperature of the core to the core forging temperature; and characterised by the following steps: driving the hub into the core to upset a first portion of the core into an outer annular groove defined in a first faying surface of the (hub, wherein, prior to driving the hub into the core, the core has a core inner diameter smaller than a smallest hub outer diameter; and driving the rim gear over the core to upset a second portion of the core into an inner annular groove defined in a second faying surface of the rim gear, wherein, prior to driving the rim gear over the core, the core has a core outer diameter larger than a largest rim gear inner diameter. providing a rim gear, a hub and a core, wherein the core is annular and has a core forging temperature below a hot hardness temperature of the rim gear and the hub; rotating, about an axis, the rim gear and the hub relative to the core, the rim gear and the hub driving the hub into the core to upset a first portion of the core into an outer annular groove defined in a first faying surface of the (hub, wherein, prior to driving the hub into the core, the core has a core inner diameter smaller than a smallest hub outer diameter; and driving the rim gear over the core to upset a second portion of the core into an inner annular groove defined in a second faying surface of the rim gear, wherein, prior to driving the rim gear over the core, the core has a core outer diameter larger than a largest rim gear inner diameter.
An optical fiber preform extending in a longitudinal direction for drawing an optical fiber therefrom having a first core, and a second core winding about the first core, comprising: a unitary core rod comprising a first core and a cladding disposed about said core; an arrangement of elongate members (653, 624) forming a ring disposed about said core rod; a second core comprising at least one of the elongate members of the arrangement forming a said ring; a tube disposed about the arrangement of elongate members; said preform being constructed and arranged such that the preform can be drawn with rotation about an axis passing through the first core to provide the fiber wherein the first core of the fiber is multimode at a selected wavelength of operation and a second core of the fiber is spaced from and winds around the first core of the fiber and has a selected longitudinal pitch and wherein at said wavelength of operation the second core of the fiber couples to a higher order mode of the first core of the fiber and increases the attenuation thereof relative to the fundamental mode of the first core of the fiber. a unitary core rod comprising a first core and a cladding disposed about said core; an arrangement of elongate members (653, 624) forming a ring disposed about said core rod; a second core comprising at least one of the elongate members of the arrangement forming a said ring; a tube disposed about the arrangement of elongate members; said preform being constructed and arranged such that the preform can be drawn with rotation about an axis passing through the first core to provide the fiber wherein the first core of the fiber is multimode at a selected wavelength of operation and a second core of the fiber is spaced from and winds around the first core of the fiber and has a selected longitudinal pitch and wherein at said wavelength of operation the second core of the fiber couples to a higher order mode of the first core of the fiber and increases the attenuation thereof relative to the fundamental mode of the first core of the fiber.
Extractor hood with an air guidance element which is inserted into the suction opening and which comprises a vapour guide plate which has a baffle plate, at least one through opening, which lies above the baffle plate, and an inflow geometry at least partially surrounding the baffle plate and having at least one inflow surface, wherein the inflow surface is upwardly inclined from the outer edge of the vapour guide plate in the direction of the through opening, characterised in that a guide surface is provided at least on one part of the edge of the baffle plate and extends upwards from the baffle plate and the angle of inclination (α) of the guide surface with respect to the horizontal is greater than or equal to the angle of inclination (β) between the inflow surface and the horizontal, wherein the vapour guide plate is formed by a single piece of bent sheet metal.