Method for detecting a target moiety in a sample of biological specimens by: a) providing at least one conjugate with the general formula X n - P - Y m, with X is an detection moiety, P an enzymatically degradable spacer and Y an antigen recognizing moiety and n, m are integers between 1 and 100 and wherein X and Y are covalently bound to P, b) contacting the sample of biological specimens with at least one conjugate, thereby labeling the target moiety recognized by the antigen recognizing moiety Y c) detecting the target moiety labeled with the conjugate with the detecting moiety X and d) enzymatically degrading spacer P by adding an enzyme, thereby cleaving the detection moiety X from the conjugate characterized by providing at least two conjugates, wherein each antigen recognizing moiety Y recognizes different antigens and wherein the detection moiety is selected from the group consisting of chromophore moiety, fluorescent moiety, phosphorescent moiety, luminescent moiety, light absorbing moiety, radioactive moiety, transition metal and isotope mass tag moiety and wherein the enzymatically degradable spacer P is selected from the group consisting of polysaccharides, depsipeptides, polyesters, nucleic acids, and wherein the antigen recognizing moiety Y is an antibody, an fragmented antibody, an fragmented antibody derivative, peptide/MHC-complexes targeting TCR molecules, cell adhesion receptor molecules, receptors for costimulatory molecules or artificial engineered binding molecules and wherein the enzyme used for degrading the enzymatically degradable spacer P is selected from the group consisting of glycosidases, dextranases, pullulanases, amylases, inulinases, cellulases, hemicellulases, pectinases, chitosanases, chitinases, esterases, lipases and nucleases. a) providing at least one conjugate with the general formula X n - P - Y m, with X is an detection moiety, P an enzymatically degradable spacer and Y an antigen recognizing moiety and n, m are integers between 1 and 100 and wherein X and Y are covalently bound to P, b) contacting the sample of biological specimens with at least one conjugate, thereby labeling the target moiety recognized by the antigen recognizing moiety Y c) detecting the target moiety labeled with the conjugate with the detecting moiety X and d) enzymatically degrading spacer P by adding an enzyme, thereby cleaving the detection moiety X from the conjugate characterized by providing at least two conjugates, wherein each antigen recognizing moiety Y recognizes different antigens and wherein the detection moiety is selected from the group consisting of chromophore moiety, fluorescent moiety, phosphorescent moiety, luminescent moiety, light absorbing moiety, radioactive moiety, transition metal and isotope mass tag moiety and wherein the enzymatically degradable spacer P is selected from the group consisting of polysaccharides, depsipeptides, polyesters, nucleic acids, and wherein the antigen recognizing moiety Y is an antibody, an fragmented antibody, an fragmented antibody derivative, peptide/MHC-complexes targeting TCR molecules, cell adhesion receptor molecules, receptors for costimulatory molecules or artificial engineered binding molecules and wherein the enzyme used for degrading the enzymatically degradable spacer P is selected from the group consisting of glycosidases, dextranases, pullulanases, amylases, inulinases, cellulases, hemicellulases, pectinases, chitosanases, chitinases, esterases, lipases and nucleases.