A method for detecting presence of Legionella nucleic acid in a sample, comprising: contacting the sample with a first probe consisting of X-CGTGGGTTGCAA-Y (SEQ ID NO: 6); contacting the sample with a second probe consisting of X-CGGAAGCAATGGCTAAAGGCATGCA-Y (SEQ ID NO: 9); contacting the sample with a third probe consisting of X-TTTATTACTCCACTCCAGCGAT-Y (SEQ ID NO: 12); wherein each of the first, second, and third probes are detectably labeled with X and Y; and detecting hybridization between one or more detectably labeled probe and a nucleic acid, wherein detection of hybridization between the first probe and a nucleic acid indicates presence of Legionella spp. in the sample, detection of hybridization between the second probe and a nucleic acid indicates presence of Legionella pneumophila in the sample, and detection of hybridization between the third probe and a nucleic acid indicates presence of Legionella pneumophila serogroup 1 in the sample, optionally wherein detecting hybridization comprises detecting a change in signal from one or more detectably labeled probe during or after hybridization relative to signal from the label before hybridization; wherein the method further comprises amplifying the Legionella spp. ssrA nucleic acid, Legionella pneumophila mip nucleic acid, and Legionella pneumophila serogroup 1 wzm nucleic acid prior to or substantially simultaneously with contacting the sample with the first, second, and third probes, wherein amplifying the Legionella spp. ssrA nucleic acid comprises contacting the sample with a primer consisting of the nucleic acid sequence set forth as SEQ ID NO: 4 and a primer consisting of the nucleic acid sequence set forth as SEQ ID NO: 5; and amplifying the Legionella pneumophila mip nucleic acid comprises contacting the sample with a primer consisting of the nucleic acid sequence set forth as SEQ ID NO: 7 and a primer consisting of the nucleic acid sequence set forth as SEQ ID NO: 8; and amplifying the Legionella pneumophila serogroup 1 wzm nucleic acid comprises contacting the sample with a primer consisting of the nucleic acid sequence set forth as SEQ ID NO: 10 and a primer consisting of the nucleic acid sequence set forth as SEQ ID NO: 11. contacting the sample with a first probe consisting of X-CGTGGGTTGCAA-Y (SEQ ID NO: 6); contacting the sample with a second probe consisting of X-CGGAAGCAATGGCTAAAGGCATGCA-Y (SEQ ID NO: 9); contacting the sample with a third probe consisting of X-TTTATTACTCCACTCCAGCGAT-Y (SEQ ID NO: 12); wherein each of the first, second, and third probes are detectably labeled with X and Y; and detecting hybridization between one or more detectably labeled probe and a nucleic acid, wherein detection of hybridization between the first probe and a nucleic acid indicates presence of Legionella spp. in the sample, detection of hybridization between the second probe and a nucleic acid indicates presence of Legionella pneumophila in the sample, and detection of hybridization between the third probe and a nucleic acid indicates presence of Legionella pneumophila serogroup 1 in the sample, optionally wherein detecting hybridization comprises detecting a change in signal from one or more detectably labeled probe during or after hybridization relative to signal from the label before hybridization; wherein the method further comprises amplifying the Legionella spp. ssrA nucleic acid, Legionella pneumophila mip nucleic acid, and Legionella pneumophila serogroup 1 wzm nucleic acid prior to or substantially simultaneously with contacting the sample with the first, second, and third probes, wherein amplifying the Legionella spp. ssrA nucleic acid comprises contacting the sample with a primer consisting of the nucleic acid sequence set forth as SEQ ID NO: 4 and a primer consisting of the nucleic acid sequence set forth as SEQ ID NO: 5; and amplifying the Legionella pneumophila mip nucleic acid comprises contacting the sample with a primer consisting of the nucleic acid sequence set forth as SEQ ID NO: 7 and a primer consisting of the nucleic acid sequence set forth as SEQ ID NO: 8; and amplifying the Legionella pneumophila serogroup 1 wzm nucleic acid comprises contacting the sample with a primer consisting of the nucleic acid sequence set forth as SEQ ID NO: 10 and a primer consisting of the nucleic acid sequence set forth as SEQ ID NO: 11.