Method of cross-talk compensation in a plurality of digital images, each digital image of the plurality of digital images containing image information about a plurality of objects, each of the plurality of objects being configured to receive at least one molecule comprising genetic information, the at least one molecule being configured to receive one of at least a first fluorescent compound and a second fluorescent compound, a first digital image of the plurality of digital images being taken by an optical imaging system during emission of electromagnetic radiation in a first frequency range for the first fluorescent compound, a second digital image of the plurality of digital images being taken by the optical imaging system during emission of electromagnetic radiation in a second frequency range for the second fluorescent compound, wherein the method comprises the following steps, preferably in the following order: a) determining a first intensity value from the first digital image for each object; b) determining a second intensity value from the second digital image for each object, the first and second intensity values defining data points, each data point comprising a first intensity value and a second intensity value; c) specifying a subset of the data points; k) grouping the subset of the data points into a plurality of groups, wherein each group of the plurality of groups comprises one representative data point; d) determining, in order to determine compensated first intensity values, a relation between the first and second intensity values on the basis of only the representative data points; e) determining compensated first intensity values on the basis of the first intensity values, the second intensity values, and the relation between the first intensity values and the second intensity values; characterized in that determining the relation between the first and second intensity values in step d) is performed with a random sample consensus algorithm. each digital image of the plurality of digital images containing image information about a plurality of objects, each of the plurality of objects being configured to receive at least one molecule comprising genetic information, the at least one molecule being configured to receive one of at least a first fluorescent compound and a second fluorescent compound, a first digital image of the plurality of digital images being taken by an optical imaging system during emission of electromagnetic radiation in a first frequency range for the first fluorescent compound, a second digital image of the plurality of digital images being taken by the optical imaging system during emission of electromagnetic radiation in a second frequency range for the second fluorescent compound, wherein the method comprises the following steps, preferably in the following order: a) determining a first intensity value from the first digital image for each object; b) determining a second intensity value from the second digital image for each object, the first and second intensity values defining data points, each data point comprising a first intensity value and a second intensity value; c) specifying a subset of the data points; k) grouping the subset of the data points into a plurality of groups, wherein each group of the plurality of groups comprises one representative data point; d) determining, in order to determine compensated first intensity values, a relation between the first and second intensity values on the basis of only the representative data points; e) determining compensated first intensity values on the basis of the first intensity values, the second intensity values, and the relation between the first intensity values and the second intensity values; a) determining a first intensity value from the first digital image for each object; b) determining a second intensity value from the second digital image for each object, the first and second intensity values defining data points, each data point comprising a first intensity value and a second intensity value; c) specifying a subset of the data points; k) grouping the subset of the data points into a plurality of groups, wherein each group of the plurality of groups comprises one representative data point; d) determining, in order to determine compensated first intensity values, a relation between the first and second intensity values on the basis of only the representative data points; e) determining compensated first intensity values on the basis of the first intensity values, the second intensity values, and the relation between the first intensity values and the second intensity values; characterized in that determining the relation between the first and second intensity values in step d) is performed with a random sample consensus algorithm.