A method of producing a desired protein, comprising: (a) culturing eukaryotic cells comprising one or more genes that provide for the expression of said desired protein at a first temperature; and (b) culturing said eukaryotic cells at a second temperature and allowing said eukaryotic cells to produce said desired protein, wherein the culturing comprises a fed batch phase; wherein: (i) said eukaryotic cells comprise yeast cells that are Pichia pastoris cells; (ii) said desired protein comprises a full-length antibody comprising two identical heavy chains and two identical light chains; (iii) said first temperature is between 27.5 degrees C and 28.5 degrees C and said second temperature is between 30 degrees C and 31 degrees C; (iv) the promoter or promoters which regulate the transcription of the one or more genes that encode said desired protein are not temperature inducible; (v) step (a) comprises culturing said eukaryotic cells in a culture medium comprising glycerol as a carbon source until said glycerol is exhausted; (vi) the method includes the addition of an ethanol bolus to the culture concurrently with the exhaustion of the glycerol; and (vii) said method increases the yield of said protein and/or decreases the relative abundance of one or more product-associated variants relative to the same method effected without a difference between said first temperature and said second temperature. (a) culturing eukaryotic cells comprising one or more genes that provide for the expression of said desired protein at a first temperature; and (b) culturing said eukaryotic cells at a second temperature and allowing said eukaryotic cells to produce said desired protein, wherein the culturing comprises a fed batch phase; wherein: (i) said eukaryotic cells comprise yeast cells that are Pichia pastoris cells; (ii) said desired protein comprises a full-length antibody comprising two identical heavy chains and two identical light chains; (iii) said first temperature is between 27.5 degrees C and 28.5 degrees C and said second temperature is between 30 degrees C and 31 degrees C; (iv) the promoter or promoters which regulate the transcription of the one or more genes that encode said desired protein are not temperature inducible; (v) step (a) comprises culturing said eukaryotic cells in a culture medium comprising glycerol as a carbon source until said glycerol is exhausted; (vi) the method includes the addition of an ethanol bolus to the culture concurrently with the exhaustion of the glycerol; and (vii) said method increases the yield of said protein and/or decreases the relative abundance of one or more product-associated variants relative to the same method effected without a difference between said first temperature and said second temperature. (i) said eukaryotic cells comprise yeast cells that are Pichia pastoris cells; (ii) said desired protein comprises a full-length antibody comprising two identical heavy chains and two identical light chains; (iii) said first temperature is between 27.5 degrees C and 28.5 degrees C and said second temperature is between 30 degrees C and 31 degrees C; (iv) the promoter or promoters which regulate the transcription of the one or more genes that encode said desired protein are not temperature inducible; (v) step (a) comprises culturing said eukaryotic cells in a culture medium comprising glycerol as a carbon source until said glycerol is exhausted; (vi) the method includes the addition of an ethanol bolus to the culture concurrently with the exhaustion of the glycerol; and (vii) said method increases the yield of said protein and/or decreases the relative abundance of one or more product-associated variants relative to the same method effected without a difference between said first temperature and said second temperature.