A substrate for sample analysis, which involves transportation of a liquid through rotational motion, the substrate for sample analysis comprising: a substrate including a rotation axis; a first chamber, which is positioned in the substrate and includes a first space configured to retain the liquid; a second chamber, which is positioned in the substrate and includes a second space configured to retain the liquid discharged from the first chamber; and a first flow passage, which is positioned in the substrate, includes a path configured to connect the first chamber and the second chamber to each other, and is capable of being filled with the liquid retained in the first space through a capillary phenomenon, wherein the first flow passage has a first opening and a second opening, the first opening and the second opening are connected to the first chamber and the second chamber, respectively, and the first opening is positioned on a side closer to the rotation axis than the second opening, wherein the first space includes a first region (101a, 101a'), which is connected to the first opening and includes a portion extending from the first opening toward a side farther from the rotation axis, wherein the first space of the first chamber has a capacity larger than a capacity of the first flow passage, wherein the first flow passage includes a first portion having the first opening and a second portion having the second opening, wherein the second portion has a capillary force larger than a capillary force of the first portion, characterized in that the first flow passage further includes a space which is adjacent to the first portion, and which is positioned on the rotation axis side from the first portion and an opening communicating to the space, and wherein the space is free from being a capillary path. a substrate including a rotation axis; a first chamber, which is positioned in the substrate and includes a first space configured to retain the liquid; a second chamber, which is positioned in the substrate and includes a second space configured to retain the liquid discharged from the first chamber; and a first flow passage, which is positioned in the substrate, includes a path configured to connect the first chamber and the second chamber to each other, and is capable of being filled with the liquid retained in the first space through a capillary phenomenon, wherein the first flow passage has a first opening and a second opening, the first opening and the second opening are connected to the first chamber and the second chamber, respectively, and the first opening is positioned on a side closer to the rotation axis than the second opening, wherein the first space includes a first region (101a, 101a'), which is connected to the first opening and includes a portion extending from the first opening toward a side farther from the rotation axis, wherein the first space of the first chamber has a capacity larger than a capacity of the first flow passage, wherein the first flow passage includes a first portion having the first opening and a second portion having the second opening, wherein the second portion has a capillary force larger than a capillary force of the first portion, characterized in that the first flow passage further includes a space which is adjacent to the first portion, and which is positioned on the rotation axis side from the first portion and an opening communicating to the space, and wherein the space is free from being a capillary path.