An apparatus, comprising: an inlet configured to be placed in fluid communication with a bodily fluid source; an outlet configured to be placed in fluid communication with a fluid collection device; a sequestration portion configured to be in fluid communication with the inlet, the sequestration portion including a vent configured to allow a flow of gas out of the sequestration portion operable to initiate a flow of a first volume of bodily fluid from the bodily fluid source into the sequestration portion; and a flow controller disposed in the sequestration portion, the flow controller including an expandable material, a seal, and an extension extending from the seal, the expandable material configured to expand in response to contact with a portion of the first volume of bodily fluid to move the seal within the sequestration portion thereby transitioning the flow controller from a first state in which the extension obstructs the outlet to a second state in which the outlet is unobstructed by the extension, the flow controller configured to allow a negative pressure differential between the sequestration portion and the inlet as the flow controller transitions from the first state to the second state such that the first volume of bodily fluid flows from the inlet into the sequestration portion, the flow controller in the second state configured to allow (i) the negative pressure differential to substantially equalize, (ii) the first volume of bodily fluid to be sequestered in the sequestration portion, and (iii) a second volume of bodily fluid to be transferred from the inlet to the outlet. an inlet configured to be placed in fluid communication with a bodily fluid source; an outlet configured to be placed in fluid communication with a fluid collection device; a sequestration portion configured to be in fluid communication with the inlet, the sequestration portion including a vent configured to allow a flow of gas out of the sequestration portion operable to initiate a flow of a first volume of bodily fluid from the bodily fluid source into the sequestration portion; and a flow controller disposed in the sequestration portion, the flow controller including an expandable material, a seal, and an extension extending from the seal, the expandable material configured to expand in response to contact with a portion of the first volume of bodily fluid to move the seal within the sequestration portion thereby transitioning the flow controller from a first state in which the extension obstructs the outlet to a second state in which the outlet is unobstructed by the extension, the flow controller configured to allow a negative pressure differential between the sequestration portion and the inlet as the flow controller transitions from the first state to the second state such that the first volume of bodily fluid flows from the inlet into the sequestration portion, the flow controller in the second state configured to allow (i) the negative pressure differential to substantially equalize, (ii) the first volume of bodily fluid to be sequestered in the sequestration portion, and (iii) a second volume of bodily fluid to be transferred from the inlet to the outlet.