A pumping apparatus for holding a tube comprising: two opposing members (11, 12, 21, 22, 31, 32, 41, 42, 110, 120, 210, 220, 310, 320, 410, 420) being set along a longitudinal direction of the tube made of an elastic material with a relation that opposing surfaces of said two opposing members (11, 12, 21, 22, 31, 32, 41, 42, 110, 120, 210, 220, 310, 320, 410, 420) oppose each other across said tube; and having grooves (13, 14, 23, 24, 33, 34, 43, 44, 111, 121, 211, 221, 321, 411, 421) each formed on each of said opposing surfaces wherein said grooves (13, 14, 23, 24, 33, 34, 43, 44, 111, 121, 211, 221, 321, 411, 421) meet to form a space that holds said tube in a cross section thereof, wherein said two opposing members (11, 12, 21, 22, 31, 32, 41, 42, 110, 120, 210, 220, 310, 320, 410, 420) have reciprocal motion, wherein the reciprocal motion is realized with a shuttle motion such that at least one of said two opposing members (11, 12, 21, 22, 31, 32, 41, 42, 110, 120, 210, 220, 310, 320, 410, 420) shuttles in parallel with an opposing surface of the other opposing member, between a liquid holding position where a liquid introduced into said tube held in said space is held therein and a liquid discharging position where said liquid introduced into said tube is discharged from said tube of which cross sectional shape is deformed by said reciprocal motion, characterized in that said reciprocal motion further has a move-in motion such that said at least one of said two opposing members (11, 12, 21, 22, 31, 32, 41, 42, 110, 120, 210, 220, 310, 320, 410, 420) vertically moves to said opposing surfaces of the other opposing member in a mutual relation that surrounding part of said groove (13, 14, 23, 24, 33, 34, 43, 44, 111, 121, 211, 221, 321, 411, 421) thereof moves into an inner space of said groove (13, 14, 23, 24, 33, 34, 43, 44, 111, 121, 211, 221, 321, 411, 421) of the other opposing member. two opposing members (11, 12, 21, 22, 31, 32, 41, 42, 110, 120, 210, 220, 310, 320, 410, 420) being set along a longitudinal direction of the tube made of an elastic material with a relation that opposing surfaces of said two opposing members (11, 12, 21, 22, 31, 32, 41, 42, 110, 120, 210, 220, 310, 320, 410, 420) oppose each other across said tube; and having grooves (13, 14, 23, 24, 33, 34, 43, 44, 111, 121, 211, 221, 321, 411, 421) each formed on each of said opposing surfaces wherein said grooves (13, 14, 23, 24, 33, 34, 43, 44, 111, 121, 211, 221, 321, 411, 421) meet to form a space that holds said tube in a cross section thereof, wherein said two opposing members (11, 12, 21, 22, 31, 32, 41, 42, 110, 120, 210, 220, 310, 320, 410, 420) have reciprocal motion, wherein the reciprocal motion is realized with a shuttle motion such that at least one of said two opposing members (11, 12, 21, 22, 31, 32, 41, 42, 110, 120, 210, 220, 310, 320, 410, 420) shuttles in parallel with an opposing surface of the other opposing member, between a liquid holding position where a liquid introduced into said tube held in said space is held therein and a liquid discharging position where said liquid introduced into said tube is discharged from said tube of which cross sectional shape is deformed by said reciprocal motion, characterized in that said reciprocal motion further has a move-in motion such that said at least one of said two opposing members (11, 12, 21, 22, 31, 32, 41, 42, 110, 120, 210, 220, 310, 320, 410, 420) vertically moves to said opposing surfaces of the other opposing member in a mutual relation that surrounding part of said groove (13, 14, 23, 24, 33, 34, 43, 44, 111, 121, 211, 221, 321, 411, 421) thereof moves into an inner space of said groove (13, 14, 23, 24, 33, 34, 43, 44, 111, 121, 211, 221, 321, 411, 421) of the other opposing member.