A method for determining the presence of a hook effect in a patient's liquid test sample to determine the accuracy of concentration measurements of at least one target analyte of interest present within the patient's liquid test sample, the method comprising the steps of: conducting at least one diagnostic assay, which is an immunoassay, on a patient's liquid test sample within a diagnostic assay reaction vessel of a diagnostic assay system to determine the concentration of at least one target analyte of interest present in the patient's liquid test sample, wherein during the conductance of the at least one diagnostic assay, at least a first absorbance measurement of the patient's liquid test sample, a second absorbance measurement of the patient's liquid test sample, and a final absorbance measurement of the patient's liquid test sample are recorded via optical interrogation of the patient's liquid test sample; wherein MR1 is a first absorbance reading taken of the target analytes at a first time (T 1); MR2 is a second absorbance of the target analyte at a second time (T 2); both of MR1 and MR2 have been taken early during an assay reagent mix cycle of the diagnostic assay in order to monitor the progress of the immunoassay reaction(s), wherein these early readings facilitate the monitoring of the reaction kinetics for the immunoassay reaction(s), which are different for hook and non-hook samples determining a final concentration result of the at least one target analyte associated with the MRC of the patient's liquid test sample; establishing whether the final concentration result of the at least one target analyte exceeds a predetermined concentration value; and if the final concentration result of the at least one target analyte exceeds a predetermined concentration value, determining the presence of a hook effect associated with the at least one target analyte of interest present in the patient's liquid test sample by utilizing the MR1, MR2, and MRC in a detection formula, the detection formula comprising the following: IF (the at least one target analyte's concentration result is ≥ C) AND ((MCR-MR2)/(MR2-MR1) > R), THEN the at least one target analyte of interest present in the patient's liquid test sample is subject to the hook effect, wherein C is the predetermined concentration value, and R is a predetermined limit for a ratio value to assess reaction kinetics of the at least one diagnostic assay, further wherein at least one result of the detection formula is output to a user. conducting at least one diagnostic assay, which is an immunoassay, on a patient's liquid test sample within a diagnostic assay reaction vessel of a diagnostic assay system to determine the concentration of at least one target analyte of interest present in the patient's liquid test sample, wherein during the conductance of the at least one diagnostic assay, at least a first absorbance measurement of the patient's liquid test sample, a second absorbance measurement of the patient's liquid test sample, and a final absorbance measurement of the patient's liquid test sample are recorded via optical interrogation of the patient's liquid test sample; wherein MR1 is a first absorbance reading taken of the target analytes at a first time (T 1); MR2 is a second absorbance of the target analyte at a second time (T 2); both of MR1 and MR2 have been taken early during an assay reagent mix cycle of the diagnostic assay in order to monitor the progress of the immunoassay reaction(s), wherein these early readings facilitate the monitoring of the reaction kinetics for the immunoassay reaction(s), which are different for hook and non-hook samples determining a final concentration result of the at least one target analyte associated with the MRC of the patient's liquid test sample; establishing whether the final concentration result of the at least one target analyte exceeds a predetermined concentration value; and if the final concentration result of the at least one target analyte exceeds a predetermined concentration value, determining the presence of a hook effect associated with the at least one target analyte of interest present in the patient's liquid test sample by utilizing the MR1, MR2, and MRC in a detection formula, the detection formula comprising the following: IF (the at least one target analyte's concentration result is ≥ C) AND ((MCR-MR2)/(MR2-MR1) > R), THEN the at least one target analyte of interest present in the patient's liquid test sample is subject to the hook effect, wherein C is the predetermined concentration value, and R is a predetermined limit for a ratio value to assess reaction kinetics of the at least one diagnostic assay, further wherein at least one result of the detection formula is output to a user. IF (the at least one target analyte's concentration result is ≥ C) AND ((MCR-MR2)/(MR2-MR1) > R), THEN the at least one target analyte of interest present in the patient's liquid test sample is subject to the hook effect, wherein C is the predetermined concentration value, and R is a predetermined limit for a ratio value to assess reaction kinetics of the at least one diagnostic assay, further wherein at least one result of the detection formula is output to a user.