A multiple unit enabled system for processing tissue in a closed pathway for maintaining sterility comprising: a centrifuge tube having an essentially cylindrical body having a top, an inverse conical apex and an interior volume and further comprising: at least two swabable valves; an extraction tube (612, 619) releasably affixed to each of the at least two swabable valves that extends a distance within the interior volume towards the inverse conical apex; a screen disposed within the interior volume of the body, the screen including a plurality of pores, the screen configured to support tissue introduced into the centrifuge tube; and a filter being positioned below the screen in the inverse conical apex of the centrifuge tube, the filter including a plurality of pores, the plurality of pores for the filter being smaller than the plurality of pores for the screen; and a container having at least one swabable valve, wherein the swabable valve can be releasably connected to one of the at least two swabable valves of the centrifuge tube for a sterile transfer of material contained with a closed system; wherein an end of the tubing (612, 619) is positioned in a section of the body between the screen and the filter; wherein the plurality of pores of the screen are sized such that upon centrifugation, viscous tissue of the biological substance is broken down into smaller particles; and wherein the plurality of pores of the filter are sized such that upon centrifugation, the micronized tissue is squeezed through the screen and collected on top of a surface of the filter while a liquid fraction is moved downward below the filter. a centrifuge tube having an essentially cylindrical body having a top, an inverse conical apex and an interior volume and further comprising: at least two swabable valves; an extraction tube (612, 619) releasably affixed to each of the at least two swabable valves that extends a distance within the interior volume towards the inverse conical apex; a screen disposed within the interior volume of the body, the screen including a plurality of pores, the screen configured to support tissue introduced into the centrifuge tube; and a filter being positioned below the screen in the inverse conical apex of the centrifuge tube, the filter including a plurality of pores, the plurality of pores for the filter being smaller than the plurality of pores for the screen; and a container having at least one swabable valve, at least two swabable valves; an extraction tube (612, 619) releasably affixed to each of the at least two swabable valves that extends a distance within the interior volume towards the inverse conical apex; a screen disposed within the interior volume of the body, the screen including a plurality of pores, the screen configured to support tissue introduced into the centrifuge tube; and a filter being positioned below the screen in the inverse conical apex of the centrifuge tube, the filter including a plurality of pores, the plurality of pores for the filter being smaller than the plurality of pores for the screen; and a container having at least one swabable valve, wherein the swabable valve can be releasably connected to one of the at least two swabable valves of the centrifuge tube for a sterile transfer of material contained with a closed system; wherein an end of the tubing (612, 619) is positioned in a section of the body between the screen and the filter; wherein the plurality of pores of the screen are sized such that upon centrifugation, viscous tissue of the biological substance is broken down into smaller particles; and wherein the plurality of pores of the filter are sized such that upon centrifugation, the micronized tissue is squeezed through the screen and collected on top of a surface of the filter while a liquid fraction is moved downward below the filter.