A method of producing a recombinant protein preparation, the method comprising: (a) providing a cell genetically engineered to express a recombinant protein; (b) culturing the cell in a culture medium comprising 0.5% to 5% DMSO and, optionally, further comprising one or more of glucosamine, lysine, ammonium, copper, putrescine, glucose, a growth factor, a vitamin, a lipid, and a peptone under conditions in which the cell expresses the recombinant protein; and (c) harvesting a preparation of the recombinant protein produced by the cell that meets a target value, relative to total glycans, of one or more of high mannose glycans, sialylated glycans, 3,3,1,0,0 glycans and fucosylated glycans, wherein (i) the target value of high mannose glycans is a level at least 20% higher than a level of high mannose glycans, relative to total glycans, in a preparation of the recombinant protein produced by culturing the cell in the medium not comprising 0.5% to 5% DMSO; (ii) the target value of high mannose glycans is 0.1% to 20% high mannose glycans; (iii) the target value of fucosylated glycans is 70% to 100% fucosylated glycans; (iv) the target value of sialylated glycans is a level at least 10% higher than a level of one or more of sialylated glycans, relative to total glycans, in a preparation of the recombinant protein produced by culturing the cell in the medium not comprising 0.5% to 5% DMSO; and/or (v) the target value of 3,3,1,0,0 glycans is a level at least 10% higher than a level of 3,3,1,0,0 glycans, relative to total glycans, in a preparation of the recombinant protein produced by culturing the cell in the medium not comprising DMSO; and, optionally, (d) evaluating the level of the one or more of high mannose glycans, sialylated glycans, 3,3,1,0,0 glycans and fucosylated glycans in the recombinant protein preparation; and/or, (e) formulating the preparation into a drug product. (a) providing a cell genetically engineered to express a recombinant protein; (b) culturing the cell in a culture medium comprising 0.5% to 5% DMSO and, optionally, further comprising one or more of glucosamine, lysine, ammonium, copper, putrescine, glucose, a growth factor, a vitamin, a lipid, and a peptone under conditions in which the cell expresses the recombinant protein; and (c) harvesting a preparation of the recombinant protein produced by the cell that meets a target value, relative to total glycans, of one or more of high mannose glycans, sialylated glycans, 3,3,1,0,0 glycans and fucosylated glycans, wherein (i) the target value of high mannose glycans is a level at least 20% higher than a level of high mannose glycans, relative to total glycans, in a preparation of the recombinant protein produced by culturing the cell in the medium not comprising 0.5% to 5% DMSO; (ii) the target value of high mannose glycans is 0.1% to 20% high mannose glycans; (iii) the target value of fucosylated glycans is 70% to 100% fucosylated glycans; (iv) the target value of sialylated glycans is a level at least 10% higher than a level of one or more of sialylated glycans, relative to total glycans, in a preparation of the recombinant protein produced by culturing the cell in the medium not comprising 0.5% to 5% DMSO; and/or (v) the target value of 3,3,1,0,0 glycans is a level at least 10% higher than a level of 3,3,1,0,0 glycans, relative to total glycans, in a preparation of the recombinant protein produced by culturing the cell in the medium not comprising DMSO; and, optionally, (i) the target value of high mannose glycans is a level at least 20% higher than a level of high mannose glycans, relative to total glycans, in a preparation of the recombinant protein produced by culturing the cell in the medium not comprising 0.5% to 5% DMSO; (ii) the target value of high mannose glycans is 0.1% to 20% high mannose glycans; (iii) the target value of fucosylated glycans is 70% to 100% fucosylated glycans; (iv) the target value of sialylated glycans is a level at least 10% higher than a level of one or more of sialylated glycans, relative to total glycans, in a preparation of the recombinant protein produced by culturing the cell in the medium not comprising 0.5% to 5% DMSO; and/or (v) the target value of 3,3,1,0,0 glycans is a level at least 10% higher than a level of 3,3,1,0,0 glycans, relative to total glycans, in a preparation of the recombinant protein produced by culturing the cell in the medium not comprising DMSO; and, optionally, (d) evaluating the level of the one or more of high mannose glycans, sialylated glycans, 3,3,1,0,0 glycans and fucosylated glycans in the recombinant protein preparation; and/or, (e) formulating the preparation into a drug product.