A method of determining an improved estimate of a true relative abundance of a target analyte in a mixed unknown sample using a nanopore device, comprising applying a voltage across a nanopore in a nanopore device to generate a detectable electronic signature and to induce translocation of charged analytes through said nanopore for a mixed unknown sample, or separately for each of a control sample and the mixed unknown sample: the control sample comprising a known relative abundance of target analytes to reference analytes, and the mixed unknown sample comprising said target analytes and said reference analytes, wherein the relative abundance of said target analytes in said sample is to be determined; generating a plurality of event signatures generated by translocation of said target analytes and said reference analytes through said nanopore for the mixed unknown sample, or for each of the control sample and the mixed unknown sample; identifying a quantity of first event signatures associated with said target analyte and a quantity of second event signatures associated with said reference analyte from said plurality of event signatures to determine a detected relative abundance of first and second event signatures for the mixed unknown sample, or for each of the control sample and the mixed unknown sample; and adjusting a detected relative abundance of said first and second event signatures in said mixed unknown sample using the detected relative abundance of said first and second event signatures in said control sample or a predetermined relative abundance of said first and second event signatures from separate prior control experiments to correct for an error in the detected relative abundance, thereby determining an improved estimate of the true relative abundance of said target analyte in said mixed unknown sample; wherein said estimate of the true relative abundance is an estimate R mix ∗ of the true ratio of said target analyte to said reference analyte in said mixed unknown sample or an estimate of the true fraction F mix ∗ of said target analytes in a population of said reference analytes and said target analytes in said mixed unknown sample, wherein said estimate R mix ∗ of the true ratio is determined by R mix ∗ = ρα, or said estimate of the true fraction F mix ∗ is determined by F mix ∗ = ρα ρα + 1, wherein the parameter ρ is an estimate for the ratio that can compensate for a false positive detection error, a false negative detection error, or both, and wherein the parameter α can be used to compensate for a capture rate constant differential between said target analyte and said reference analyte, wherein ρ = Q mix − Q ref Q targ − Q mix, and α = Q targ − Q X: Y Q X: Y − Q ref × X Y; wherein each of the Q targ, Q ref and Q X:Y is a fraction predetermined or observed by the method. applying a voltage across a nanopore in a nanopore device to generate a detectable electronic signature and to induce translocation of charged analytes through said nanopore for a mixed unknown sample, or separately for each of a control sample and the mixed unknown sample: the control sample comprising a known relative abundance of target analytes to reference analytes, and the mixed unknown sample comprising said target analytes and said reference analytes, wherein the relative abundance of said target analytes in said sample is to be determined; the control sample comprising a known relative abundance of target analytes to reference analytes, and the mixed unknown sample comprising said target analytes and said reference analytes, wherein the relative abundance of said target analytes in said sample is to be determined; generating a plurality of event signatures generated by translocation of said target analytes and said reference analytes through said nanopore for the mixed unknown sample, or for each of the control sample and the mixed unknown sample; identifying a quantity of first event signatures associated with said target analyte and a quantity of second event signatures associated with said reference analyte from said plurality of event signatures to determine a detected relative abundance of first and second event signatures for the mixed unknown sample, or for each of the control sample and the mixed unknown sample; and adjusting a detected relative abundance of said first and second event signatures in said mixed unknown sample using the detected relative abundance of said first and second event signatures in said control sample or a predetermined relative abundance of said first and second event signatures from separate prior control experiments to correct for an error in the detected relative abundance, thereby determining an improved estimate of the true relative abundance of said target analyte in said mixed unknown sample;