A method of producing an anti-CD38 IgG1 antibody expressed from a polynucleotide encoding a heavy chain variable region of SEQ ID NO: 7 and a light chain variable region of SEQ ID NO: 8 having a G1F oligosaccharide content between 15% and 27%, comprising: i) preparing a culture medium comprising between 2 parts per billion (ppb) and 8.5 ppb manganese (Mn); and ii) controlling the G1F oligosaccharide content of the anti-CD38 antibody by culturing a eukaryotic host cell comprising the polynucleotide encoding the VH of SEQ ID NO: 7 and the VL of SEQ ID NO: 8 in the culture medium prepared in step a), thereby producing the anti-CD38 antibody expressed from the polynucleotide encoding the VH of SEQ ID NO: 7 and the VL of SEQ ID NO: 8 having the G1F oligosaccharide content between 15% and 27%; or i) culturing a eukaryotic host cell expressing the polynucleotide encoding the VH of SEQ ID NO: 7 and the VL of SEQ ID NO: 8 in conditions that the anti-CD38 antibody is produced; and ii) controlling the G1F oligosaccharide content of the anti-CD38 antibody by monitoring the concentration of Mn in the culture medium during biosynthesis of the anti-CD38 antibody and regulating the concentration of Mn in the culture medium during biosynthesis of the anti-CD38 antibody, wherein the concentration of Mn in the culture medium is regulated to comprise between 2 ppb and 8.5 ppb Mn, thereby producing the anti-CD38 antibody having the G1F oligosaccharide content between 15% and 27%. i) preparing a culture medium comprising between 2 parts per billion (ppb) and 8.5 ppb manganese (Mn); and ii) controlling the G1F oligosaccharide content of the anti-CD38 antibody by culturing a eukaryotic host cell comprising the polynucleotide encoding the VH of SEQ ID NO: 7 and the VL of SEQ ID NO: 8 in the culture medium prepared in step a), thereby producing the anti-CD38 antibody expressed from the polynucleotide encoding the VH of SEQ ID NO: 7 and the VL of SEQ ID NO: 8 having the G1F oligosaccharide content between 15% and 27%; or i) preparing a culture medium comprising between 2 parts per billion (ppb) and 8.5 ppb manganese (Mn); and ii) controlling the G1F oligosaccharide content of the anti-CD38 antibody by culturing a eukaryotic host cell comprising the polynucleotide encoding the VH of SEQ ID NO: 7 and the VL of SEQ ID NO: 8 in the culture medium prepared in step a), thereby producing the anti-CD38 antibody expressed from the polynucleotide encoding the VH of SEQ ID NO: 7 and the VL of SEQ ID NO: 8 having the G1F oligosaccharide content between 15% and 27%; or i) culturing a eukaryotic host cell expressing the polynucleotide encoding the VH of SEQ ID NO: 7 and the VL of SEQ ID NO: 8 in conditions that the anti-CD38 antibody is produced; and ii) controlling the G1F oligosaccharide content of the anti-CD38 antibody by monitoring the concentration of Mn in the culture medium during biosynthesis of the anti-CD38 antibody and regulating the concentration of Mn in the culture medium during biosynthesis of the anti-CD38 antibody, wherein the concentration of Mn in the culture medium is regulated to comprise between 2 ppb and 8.5 ppb Mn, thereby producing the anti-CD38 antibody having the G1F oligosaccharide content between 15% and 27%. i) culturing a eukaryotic host cell expressing the polynucleotide encoding the VH of SEQ ID NO: 7 and the VL of SEQ ID NO: 8 in conditions that the anti-CD38 antibody is produced; and ii) controlling the G1F oligosaccharide content of the anti-CD38 antibody by monitoring the concentration of Mn in the culture medium during biosynthesis of the anti-CD38 antibody and regulating the concentration of Mn in the culture medium during biosynthesis of the anti-CD38 antibody, wherein the concentration of Mn in the culture medium is regulated to comprise between 2 ppb and 8.5 ppb Mn, thereby producing the anti-CD38 antibody having the G1F oligosaccharide content between 15% and 27%.