A guiding system for ultrasound scanning operation, the guiding system for ultrasound scanning operation comprising: a handheld guiding device (11, 61), located at a guiding end; a display device, located at the guiding end; an ultrasound scanning device, located at an operating end; a prompting device, located at the operating end; and a control host, communicatively connected between the guiding end and the operating end, wherein when the handheld guiding device (11, 61) generates a first physical motion, the control host detects the first physical motion and generates navigation prompting information accordingly, the prompting device is suitable for presenting the navigation prompting information to guide the ultrasound scanning device to move to generate a second physical motion, and the control host captures an ultrasound image via the ultrasound scanning device and sends the ultrasound image to the display device at the guiding end for display, the guiding system for ultrasound scanning operation being characterized in that the handheld guiding device (11, 61) further comprises: a main body and a soft material layer, disposed on the main body, wherein the main body is configured to send a first inertial measurement signal to the control host according to the first physical motion of the handheld guiding device (11, 61), and the first physical motion contains the moving trajectory of the main body pressing the soft material layer to cause the soft material layer to be deformed. a handheld guiding device (11, 61), located at a guiding end; a display device, located at the guiding end; an ultrasound scanning device, located at an operating end; a prompting device, located at the operating end; and a control host, communicatively connected between the guiding end and the operating end, wherein when the handheld guiding device (11, 61) generates a first physical motion, the control host detects the first physical motion and generates navigation prompting information accordingly, the prompting device is suitable for presenting the navigation prompting information to guide the ultrasound scanning device to move to generate a second physical motion, and the control host captures an ultrasound image via the ultrasound scanning device and sends the ultrasound image to the display device at the guiding end for display, the guiding system for ultrasound scanning operation being characterized in that the handheld guiding device (11, 61) further comprises: a main body and a soft material layer, disposed on the main body, wherein the main body is configured to send a first inertial measurement signal to the control host according to the first physical motion of the handheld guiding device (11, 61), and the first physical motion contains the moving trajectory of the main body pressing the soft material layer to cause the soft material layer to be deformed. a main body and a soft material layer, disposed on the main body, wherein the main body is configured to send a first inertial measurement signal to the control host according to the first physical motion of the handheld guiding device (11, 61), and the first physical motion contains the moving trajectory of the main body pressing the soft material layer to cause the soft material layer to be deformed.