A display substrate (110, 220, 270, 290, 300, 400), comprising: a plurality of sub-pixel units arranged in a plurality of rows and a plurality of columns, wherein the plurality of columns of the sub-pixel units comprise alternatingly arranged solid color sub-pixel unit columns and mixed color sub-pixel unit columns (212, 214), the solid color sub-pixel unit columns including a plurality of sub-pixel units corresponding to a same color, wherein adjacent two sub-pixel units in each of the mixed color sub-pixel unit columns (212, 214) correspond to different colors, and sub-pixel units on each side of a sub-pixel unit in a solid color sub-pixel unit column are of different colors; wherein in the pixel unit, the at least one sub-pixel unit of the solid color sub-pixel unit column is a green sub-pixel unit, and the at least one sub-pixel unit of the mixed color sub-pixel unit column is a blue sub-pixel unit or a red sub-pixel unit, wherein the pitch is a distance between adjacent edges of the two sub-pixel units in adjacent rows; characterised in that in a pixel unit, at least one sub-pixel unit has a relationship with at least one sub-pixel unit of a different color of an adjacent row, the relationship including a pitch (D1, D3) of two sub-pixel units in a row direction being smaller than a pitch (D2, D4) of two sub-pixel units in a column direction, and an opening shape of the green sub-pixel unit is an ellipse, an angle is formed between a long axis of the ellipse and the column direction, and a direction of a long axis of any one of the green sub-pixel units has an opposite tilt direction to a tilt direction of a long axis of an adjacent green sub-pixel unit. a plurality of sub-pixel units arranged in a plurality of rows and a plurality of columns, wherein the plurality of columns of the sub-pixel units comprise alternatingly arranged solid color sub-pixel unit columns and mixed color sub-pixel unit columns (212, 214), the solid color sub-pixel unit columns including a plurality of sub-pixel units corresponding to a same color, wherein adjacent two sub-pixel units in each of the mixed color sub-pixel unit columns (212, 214) correspond to different colors, and sub-pixel units on each side of a sub-pixel unit in a solid color sub-pixel unit column are of different colors; wherein in the pixel unit, the at least one sub-pixel unit of the solid color sub-pixel unit column is a green sub-pixel unit, and the at least one sub-pixel unit of the mixed color sub-pixel unit column is a blue sub-pixel unit or a red sub-pixel unit, wherein the pitch is a distance between adjacent edges of the two sub-pixel units in adjacent rows; characterised in that in a pixel unit, at least one sub-pixel unit has a relationship with at least one sub-pixel unit of a different color of an adjacent row, the relationship including a pitch (D1, D3) of two sub-pixel units in a row direction being smaller than a pitch (D2, D4) of two sub-pixel units in a column direction, and an opening shape of the green sub-pixel unit is an ellipse, an angle is formed between a long axis of the ellipse and the column direction, and a direction of a long axis of any one of the green sub-pixel units has an opposite tilt direction to a tilt direction of a long axis of an adjacent green sub-pixel unit.