A recombinant host wherein the host is a microorganism, a plant or a plant cell capable of producing one or more steviol glycosides comprising a recombinant gene encoding a polypeptide having at least 80% sequence identity to the amino acid sequence set forth in SEQ ID NO: 152; the host further comprising one or more of: (a) a recombinant gene encoding a UGT85C2 polypeptide having approximately 90% or greater identity to the amino acid sequence set forth in SEQ ID NO:3; (b) a recombinant gene encoding a UGT76G1 polypeptide having approximately 90% or greater identity to the amino acid sequence set forth in SEQ ID NO:7; (c) a gene encoding a UGT74G1 polypeptide such as a recombinant gene; (d) a recombinant gene encoding a UGT91 D2 functional homolog; (e) a recombinant gene encoding a steviol synthase polypeptide; (f) a recombinant gene encoding an ent-copalyl diphosphate synthase (CDPS) polypeptide; (g) one or more recombinant genes encoding a sucrose transporter and a sucrose synthase; (h) a recombinant gene encoding a cytochrome P450 reductase polypeptide; (i) a nucleic acid sequence, said nucleic acid comprising a heterologous insert sequence operably linked to an open reading frame encoding a squalene synthase, wherein the heterologous insert sequence has a formula: − X 1 − X 2 − X 3 − X 4 − X 5 − wherein X2 comprises at least 4 consecutive nucleotides complementary to at least 4 consecutive nucleotides of X4, wherein X3 comprises zero nucleotides or one or more nucleotides forming a hairpin loop, wherein X1 and X5 each individually consists of zero nucleotides or one or more nucleotides; (j) one or more recombinant genes encoding a kaurene oxidase polypeptide; (k) a recombinant gene encoding a kaurene synthase polypeptide; or (l) a recombinant gene encoding a geranylgeranyl diphosphate synthase (GGPPS) polypeptide. (a) a recombinant gene encoding a UGT85C2 polypeptide having approximately 90% or greater identity to the amino acid sequence set forth in SEQ ID NO:3; (b) a recombinant gene encoding a UGT76G1 polypeptide having approximately 90% or greater identity to the amino acid sequence set forth in SEQ ID NO:7; (c) a gene encoding a UGT74G1 polypeptide such as a recombinant gene; (d) a recombinant gene encoding a UGT91 D2 functional homolog; (e) a recombinant gene encoding a steviol synthase polypeptide; (f) a recombinant gene encoding an ent-copalyl diphosphate synthase (CDPS) polypeptide; (g) one or more recombinant genes encoding a sucrose transporter and a sucrose synthase; (h) a recombinant gene encoding a cytochrome P450 reductase polypeptide; (i) a nucleic acid sequence, said nucleic acid comprising a heterologous insert sequence operably linked to an open reading frame encoding a squalene synthase, wherein the heterologous insert sequence has a formula: − X 1 − X 2 − X 3 − X 4 − X 5 − wherein X2 comprises at least 4 consecutive nucleotides complementary to at least 4 consecutive nucleotides of X4, wherein X3 comprises zero nucleotides or one or more nucleotides forming a hairpin loop, wherein X1 and X5 each individually consists of zero nucleotides or one or more nucleotides; (j) one or more recombinant genes encoding a kaurene oxidase polypeptide; (k) a recombinant gene encoding a kaurene synthase polypeptide; or (l) a recombinant gene encoding a geranylgeranyl diphosphate synthase (GGPPS) polypeptide.