mhurhangee/ep-ind-claims huggingface dataset:
A computer-implemented method of identifying biological molecules with enhanced desired activity, the method comprising: (a) receiving sequence and activity data for a plurality of biological molecules; (b) preparing a base model from the sequence and activity data, wherein the base model predicts activity as a function of the presence or absence of sub-units of the sequence and the base model includes no interaction terms from a defined pool of interaction terms; setting a current sequence model and a best sequence model to the base model; creating a new sequence model by adding to the current sequence model an interaction term not yet added from the defined pool of interaction terms; evaluating the predictive power of the new sequence model, and if the predictive power of the new sequence model is greater than that of the best sequence model, setting the new sequence model as the best sequence model; if there is any interaction term in the defined pool of interaction terms which has not been added to the current sequence model, repeating -; (d) if a new model was set as best sequence model in, setting the current sequence model to the best sequence model and repeating -(d); (e) setting the best sequence model as a final model; and (f) using the final model to guide directed evolution and to select one or more sequences with enhanced desired activity, characterized in that the interaction term is selected randomly in step. (a) receiving sequence and activity data for a plurality of biological molecules; (b) preparing a base model from the sequence and activity data, wherein the base model predicts activity as a function of the presence or absence of sub-units of the sequence and the base model includes no interaction terms from a defined pool of interaction terms; setting a current sequence model and a best sequence model to the base model; creating a new sequence model by adding to the current sequence model an interaction term not yet added from the defined pool of interaction terms; evaluating the predictive power of the new sequence model, and if the predictive power of the new sequence model is greater than that of the best sequence model, setting the new sequence model as the best sequence model; if there is any interaction term in the defined pool of interaction terms which has not been added to the current sequence model, repeating -; (d) if a new model was set as best sequence model in, setting the current sequence model to the best sequence model and repeating -(d); (e) setting the best sequence model as a final model; and (f) using the final model to guide directed evolution and to select one or more sequences with enhanced desired activity, characterized in that the interaction term is selected randomly in step.
An article of footwear, comprising: a support member comprising a bladder with an outer chamber and inner chamber, wherein the outer chamber is sealed from the inner chamber; a first fluid in the form of a gas or liquid being sealed within the outer chamber, between an exterior bladder wall and an interior bladder wall, and the inner chamber being filled with a rheological fluid; a reservoir in fluid communication with the inner chamber; and an electromagnetic device associated with the support member, wherein the electromagnetic device is configured to be activated to vary the viscosity of the rheological fluid in the inner chamber, wherein the support member includes an upper bladder wall and a lower bladder wall that are joined to the outer chamber, and the upper bladder wall, the lower bladder wall and the outer chamber bound the inner chamber, and the lower bladder wall includes a fluid port, and a fluid line facilitates fluid communication between fluid port and the reservoir. a support member comprising a bladder with an outer chamber and inner chamber, wherein the outer chamber is sealed from the inner chamber; a first fluid in the form of a gas or liquid being sealed within the outer chamber, between an exterior bladder wall and an interior bladder wall, and the inner chamber being filled with a rheological fluid; a reservoir in fluid communication with the inner chamber; and an electromagnetic device associated with the support member, wherein the electromagnetic device is configured to be activated to vary the viscosity of the rheological fluid in the inner chamber, wherein the support member includes an upper bladder wall and a lower bladder wall that are joined to the outer chamber, and the upper bladder wall, the lower bladder wall and the outer chamber bound the inner chamber, and the lower bladder wall includes a fluid port, and a fluid line facilitates fluid communication between fluid port and the reservoir.
A method for sequential loading of multiple delivery vectors onto an autonomously replicating nucleic acid in a cell by removal and reuse of a recyclable selectable marker and drug resistant gene cassette comprising: integrating a first delivery vector comprising a recyclable selectable marker gene and a drug resistant gene cassette and a first nucleic acid of interest onto the autonomously replicating nucleic acid using a first recombination system; excising the recyclable cassette from the autonomously replicating nucleic acid using a second recombination system with signal sites alpha, wherein the first nucleic acid of interest remains on the autonomously replicating nucleic acid; integrating a second delivery vector comprising the recyclable cassette and a second nucleic acid of interest onto the autonomously replicating nucleic acid using the first recombination system; and excising the recyclable cassette from the autonomously replicating nucleic acid using the second recombination system with signal sites beta, wherein the second nucleic acid of interest remains on the autonomously replicating nucleic acid. integrating a first delivery vector comprising a recyclable selectable marker gene and a drug resistant gene cassette and a first nucleic acid of interest onto the autonomously replicating nucleic acid using a first recombination system; excising the recyclable cassette from the autonomously replicating nucleic acid using a second recombination system with signal sites alpha, wherein the first nucleic acid of interest remains on the autonomously replicating nucleic acid; integrating a second delivery vector comprising the recyclable cassette and a second nucleic acid of interest onto the autonomously replicating nucleic acid using the first recombination system; and excising the recyclable cassette from the autonomously replicating nucleic acid using the second recombination system with signal sites beta, wherein the second nucleic acid of interest remains on the autonomously replicating nucleic acid.
A temperature regulation assembly comprising an energy saving module, a temperature regulating system and a temperature sensor, said energy saving module comprising connection means for connecting to said temperature regulating system and to said temperature sensor, the regulating system being associated with air conditioning means and being configured to maintain the temperature of a cabin of a passenger transportation vehicle at a setpoint temperature according to a temperature measured by the temperature sensor corresponding to the temperature of the cabin, said assembly being characterized in that the energy saving module comprises temperature shifting means able to shift said temperature measured by the temperature sensor by a predetermined value, said temperature measured by the temperature sensor being incremented when the air conditioning means are configured to heat and said temperature measured by the temperature sensor being reduced when the air conditioning means are configured to cool.
An aluminum alloy clad sheet for tube material of heat exchangers comprising a core material and a cladding material 1, one side of the core material being clad with the cladding material 1, the core material comprising 0.5 to 1.2% (mass %, hereinafter the same) of Si, 0.2 to 1.0% of Cu, 1.0 to 1.8% of Mn, 0.05 to 0.3% of Ti, optionally 0.1 to 0.2% Fe, and optionally at least one element selected from the group consisting of 0.3% or less of Cr, 0.3% or less of Zr and 0.5% or less of Mg, with the balance being Al and unavoidable impurities, and the cladding material 1 comprising 3 to 6% of Si, 2 to 8% of Zn, an Ni content of less than 0.05%, optionally 0.1 to 0.2% Fe, and at least one of 0.3 to 1.8% of Mn and 0.05 to 0.3% of Ti, and optionally at least one element selected from the group consisting of 0.005 to 0.05% of Sr, 0.3% or less of Cr, 0.3% or less of Zr, 0.001 to 0.1 % of In and 0.001 to 0.1 % of Sn, with the balance being Al and unavoidable impurities, characterized in that the aluminum alloy clad sheet is formed so that the cladding material 1 forms a convex surface and the core material forms a concave surface.
Process for determining the correct positioning of an autostereoscopic tablet computer used to perform fixation disparity measurements in a subject through haploscopic observation of stimuli, said autostereoscopic tablet computer comprising: - frontal video camera means for capturing images of the face of the subject, said frontal video camera means defining an optical axis, - a monitor having at least 1920 x 1200 pixels with a resolution of at least 100 pixels per mm 2, the monitor defining a vertical transverse median plane containing said optical axis, - image reproduction means for reproducing images captured by said video camera means on said monitor, and - dedicated software; wherein a fixation disparity measurement is performed by presenting to the subject being examined, on said autostereoscopic tablet computer, stimuli perceived by one eye and other stimuli perceived by the other eye, wherein said measurement comprises the steps consisting of: - providing a static image, on said monitor, of an upper index and a lower index, aligned according to said vertical transverse median plane; - providing at least one image, on said monitor, of a stimulus characteristic of the measurement; - when said at least one image of the stimulus is provided on said monitor, capturing an image of the face of the subject being examined, by means of said frontal video camera means and displaying this image on the monitor, in the background with respect to said image of the stimulus with sufficient contrast to perceive said image of the face in the background, wherein, for achieving correct positioning of the autostereoscopic tablet computer, said autostereoscopic tablet computer is oriented and arranged by positioning said indices (11a, 11b) the one just above the stimulus and the other just below the stimulus, so that the upper index is positioned on the central and upper part of the forehead of the subject being examined and the lower index is positioned on the tip of the nose of the same subject. - frontal video camera means for capturing images of the face of the subject, said frontal video camera means defining an optical axis, - a monitor having at least 1920 x 1200 pixels with a resolution of at least 100 pixels per mm 2, the monitor defining a vertical transverse median plane containing said optical axis, - image reproduction means for reproducing images captured by said video camera means on said monitor, and - dedicated software; - providing a static image, on said monitor, of an upper index and a lower index, aligned according to said vertical transverse median plane; - providing at least one image, on said monitor, of a stimulus characteristic of the measurement; - when said at least one image of the stimulus is provided on said monitor, capturing an image of the face of the subject being examined, by means of said frontal video camera means and displaying this image on the monitor, in the background with respect to said image of the stimulus with sufficient contrast to perceive said image of the face in the background,
A method for a user equipment, UE (104, 106, 108, 110, 112, 114; 502, 504, 506, 508, 510, 512, 514, 516), comprising: receiving, by the UE (104, 106, 108, 110, 112, 114; 502, 504, 506, 508, 510, 512, 514, 516) from a base station, BS, via a unicast high level signaling, configuration information that enables a grant-free transmission without using a request/grant mechanism, wherein the configuration information comprises pilot information; and transmitting, by the UE (104, 106, 108, 110, 112, 114; 502, 504, 506, 508, 510, 512, 514, 516) to the BS, an uplink data transmission using a pilot configured by the pilot information, wherein the grant-free transmission is a transmission without using the request/grant mechanism to obtain an allocation of transmission resources from the BS, and wherein the transmission resources include the pilot, a time resource, and a frequency resource. receiving, by the UE (104, 106, 108, 110, 112, 114; 502, 504, 506, 508, 510, 512, 514, 516) from a base station, BS, via a unicast high level signaling, configuration information that enables a grant-free transmission without using a request/grant mechanism, wherein the configuration information comprises pilot information; and transmitting, by the UE (104, 106, 108, 110, 112, 114; 502, 504, 506, 508, 510, 512, 514, 516) to the BS, an uplink data transmission using a pilot configured by the pilot information, wherein the grant-free transmission is a transmission without using the request/grant mechanism to obtain an allocation of transmission resources from the BS, and wherein the transmission resources include the pilot, a time resource, and a frequency resource.
A method for preparing genomic DNA (gDNA) samples for the detection of a single nucleotide polymorphism, the method comprising: providing a sample of buccal epithelial cells from a subject, the buccal epithelial cells adhered to a substrate which is capable of physically interacting directly with the body in order to reversibly bind the buccal cells to the substrate; agitating, for no more than about 45 seconds, the substrate with the adhered epithelial cells in a first lysis solution capable of lysing buccal cells adhered to the substrate for no more than about 45 seconds; removing the substrate from the first lysis solution upon completion of the agitating the substrate in the first lysis solution; performing an extraction incubation of gDNA in the first lysis solution by incubating the first lysis solution at a temperature of 45±3 °C for 45±15 minutes after removing the substrate from the first lysis solution to prepare a first gDNA sample; agitating the substrate, after removing the substrate from the first lysis solution, in a second lysis solution capable of lysing buccal cells adhered to the substrate; removing the substrate from the second lysis solution; and performing an extraction incubation of second gDNA in the second lysis solution by incubating the second lysis solution at a temperature of 45±3 °C for 45±15 minutes after removing the substrate from the second lysis solution to prepare a second gDNA sample. providing a sample of buccal epithelial cells from a subject, the buccal epithelial cells adhered to a substrate which is capable of physically interacting directly with the body in order to reversibly bind the buccal cells to the substrate; agitating, for no more than about 45 seconds, the substrate with the adhered epithelial cells in a first lysis solution capable of lysing buccal cells adhered to the substrate for no more than about 45 seconds; removing the substrate from the first lysis solution upon completion of the agitating the substrate in the first lysis solution; performing an extraction incubation of gDNA in the first lysis solution by incubating the first lysis solution at a temperature of 45±3 °C for 45±15 minutes after removing the substrate from the first lysis solution to prepare a first gDNA sample; agitating the substrate, after removing the substrate from the first lysis solution, in a second lysis solution capable of lysing buccal cells adhered to the substrate; removing the substrate from the second lysis solution; and performing an extraction incubation of second gDNA in the second lysis solution by incubating the second lysis solution at a temperature of 45±3 °C for 45±15 minutes after removing the substrate from the second lysis solution to prepare a second gDNA sample.
A compound of the formula I shown below wherein: R 1, R 2, R 3, R 4, R 5, R 6, R 7, R 8, R 9, R 10, R 11 and R 12 are methyl; Q is a -CH 2 -CH 2 - bridging group; X is selected from zirconium, titanium or hafnium; and each Y group is selected from halo, hydride, a phosphonated or sulphonated anion, or a alkyl, alkoxy, aryl or aryloxy group which is optionally substituted with halo, nitro, amino, phenyl, alkoxy, or Si[ alkyl] 3. R 1, R 2, R 3, R 4, R 5, R 6, R 7, R 8, R 9, R 10, R 11 and R 12 are methyl; Q is a -CH 2 -CH 2 - bridging group; X is selected from zirconium, titanium or hafnium; and each Y group is selected from halo, hydride, a phosphonated or sulphonated anion, or a alkyl, alkoxy, aryl or aryloxy group which is optionally substituted with halo, nitro, amino, phenyl, alkoxy, or Si[ alkyl] 3.
A quick connection device, comprising a sleeve configured to be connected with a first member to be connected and a moving assembly movably disposed in the sleeve along an axial direction of the sleeve, wherein the moving assembly comprises: a support base disposed in the sleeve; a mounting base, configured to be connected with a second member to be connected and movably disposed along the axial direction relative to the support base; a locking member, fixed to the mounting base and configured to cooperate with the support base to achieve a locking state and an unlocking state, and configured to restrict movement of the mounting base towards a direction away from the support base in the locking state and configured to allow movement of the mounting base towards a direction away from the support base in the unlocking state; and a limiting member, configured to restrict movement of the moving assembly in the sleeve when the mounting base is in an initial position corresponding to the locking state, and configured to release limiting when the mounting base is configured to move a preset distance towards the direction away from the support base, in order to allow the moving assembly to move out of the sleeve. a support base disposed in the sleeve; a mounting base, configured to be connected with a second member to be connected and movably disposed along the axial direction relative to the support base; a locking member, fixed to the mounting base and configured to cooperate with the support base to achieve a locking state and an unlocking state, and configured to restrict movement of the mounting base towards a direction away from the support base in the locking state and configured to allow movement of the mounting base towards a direction away from the support base in the unlocking state; and a limiting member, configured to restrict movement of the moving assembly in the sleeve when the mounting base is in an initial position corresponding to the locking state, and configured to release limiting when the mounting base is configured to move a preset distance towards the direction away from the support base, in order to allow the moving assembly to move out of the sleeve.
A device for peeling beans and separating the hulls from peeled beans, characterized by and comprising a rough disc inside a bowl wherein said disc is suitable for abrasively impacting beans in water and intended to rip off the hulls from the beans within a peeling region bounded by a barrier ring which prevents the beans but allows the hulls to pass to a collection region where the hulls may be trapped by a special filter, and an electric motor for driving the rough disc.
A method for treating hair comprising: A) applying a composition A comprising at least one pigment to a first portion of the hair having a first initial colour to impart the first portion of the hair with a second colour different to the first initial colour, wherein the at least one pigment has a D 50 particle diameter of 60 nm to 1 μm, and wherein the difference between the L* value of the second colour and the L* value of the first initial colour L ∗ second colour - L ∗ initial first colour is ≥ 10, particularly ≥ 15 or such as ≥ 20, according to the CIE L* a* b* system, and prior to step A): B) providing in at least a second portion of the hair one or more polymeric sublayer(s), wherein the first and second portions of the hair have at least one first common area, wherein step B) comprises B1) applying a composition B1 comprising one or more cationic polymer(s) to the second portion of the hair. A) applying a composition A comprising at least one pigment to a first portion of the hair having a first initial colour to impart the first portion of the hair with a second colour different to the first initial colour, wherein the at least one pigment has a D 50 particle diameter of 60 nm to 1 μm, and wherein the difference between the L* value of the second colour and the L* value of the first initial colour L ∗ second colour - L ∗ initial first colour is ≥ 10, particularly ≥ 15 or such as ≥ 20, according to the CIE L* a* b* system,
Microemulsion concentrates for impregnating textile and paper in cosmetics, comprising a1) alkyl and/or alkenyl glycosides a2) cosurfactants selected from the group formed of monoesters of glycerol with a C 6 -C 22 fatty acid in quantities of 3 to 20 wt% - based on concentrate a3) oils other than a2) a4) amphoteric or zwitterionic surfactant selected from the group of amphoacetates in quantities of 4.75 to 6.5 wt% - based on concentrate - and a5) water, wherein the total water content is 0.5 - 35 wt% - based on concentrate. a1) alkyl and/or alkenyl glycosides a2) cosurfactants selected from the group formed of monoesters of glycerol with a C 6 -C 22 fatty acid in quantities of 3 to 20 wt% - based on concentrate a3) oils other than a2) a4) amphoteric or zwitterionic surfactant selected from the group of amphoacetates in quantities of 4.75 to 6.5 wt% - based on concentrate - and a5) water,
A mandrel for holding an ophthalmic lens member blank (52, 54) during machining, the mandrel comprising: a front surface for receiving an ophthalmic lens member blank to be machined; a rear surface opposite the front surface; and one or more through-holes (10, 10a, 10b) extending between the front and rear surfaces; such that, in use, wax applied to the rear surface contacts an ophthalmic lens member blank received on the front surface via said one or more through-holes; wherein the mandrel comprises a stem: for mounting the mandrel on a machine tool and a flange extending radially from the stem, the flange comprising the front surface and the rear surface, the mandrel characterised in that the flange comprises the one or more through-holes. a front surface for receiving an ophthalmic lens member blank to be machined; a rear surface opposite the front surface; and one or more through-holes (10, 10a, 10b) extending between the front and rear surfaces; such that, in use, wax applied to the rear surface contacts an ophthalmic lens member blank received on the front surface via said one or more through-holes;