A dispensing method which dispenses a first liquid contained in a first vessel which stores the first liquid and a second liquid not miscible with the first liquid and having lower specific gravity than that of the first liquid, and a third liquid not miscible with the second liquid and having higher specific gravity than that of the second liquid and contained in a third vessel which stores the second liquid and the third liquid so as to introduce the first liquid and the third liquid into a second vessel by using a tube, comprising: a second suction step which sucks the first liquid contained in the first vessel to introduce this first liquid into the tube through the one end of the tube; a third suction step which sucks the second liquid contained in the first vessel to introduce this second liquid into the tube through the one end of the tube; a fourth suction step which sucks the third liquid contained in the third vessel to introduce this third liquid into the tube through the one end of the tube; and a fifth suction step which sucks the second liquid contained in the third vessel to introduce this second liquid into the tube through the one end of the tube; characterized in that the method further comprises: a first suction step which sucks the second liquid to introduce this second liquid into the tube through one end of the tube; a first discharge step which releases the second liquid sucked into the tube in the fifth suction step to discharge this second liquid into the second vessel; a second discharge step which releases the third liquid sucked into the tube in the fourth suction step to discharge this third liquid into the second vessel; a third discharge step which releases a part of the second liquid sucked into the tube in the third suction step to discharge this second liquid into the second vessel; a fourth discharge step which releases the second liquid remaining within the tube after the third discharge step to discharge this second liquid into the second vessel; a fifth discharge step which releases the first liquid sucked into the tube in the second suction step to discharge this first liquid into the second vessel; and a sixth discharge step which releases at least a part of the second liquid sucked into the tube in the first suction step to discharge this second liquid into the second vessel; wherein the second liquid is contained in the second vessel beforehand. a second suction step which sucks the first liquid contained in the first vessel to introduce this first liquid into the tube through the one end of the tube; a third suction step which sucks the second liquid contained in the first vessel to introduce this second liquid into the tube through the one end of the tube; a fourth suction step which sucks the third liquid contained in the third vessel to introduce this third liquid into the tube through the one end of the tube; and a fifth suction step which sucks the second liquid contained in the third vessel to introduce this second liquid into the tube through the one end of the tube; characterized in that the method further comprises: a first suction step which sucks the second liquid to introduce this second liquid into the tube through one end of the tube; a first discharge step which releases the second liquid sucked into the tube in the fifth suction step to discharge this second liquid into the second vessel; a second discharge step which releases the third liquid sucked into the tube in the fourth suction step to discharge this third liquid into the second vessel; a third discharge step which releases a part of the second liquid sucked into the tube in the third suction step to discharge this second liquid into the second vessel; a fourth discharge step which releases the second liquid remaining within the tube after the third discharge step to discharge this second liquid into the second vessel; a fifth discharge step which releases the first liquid sucked into the tube in the second suction step to discharge this first liquid into the second vessel; and a sixth discharge step which releases at least a part of the second liquid sucked into the tube in the first suction step to discharge this second liquid into the second vessel; a first suction step which sucks the second liquid to introduce this second liquid into the tube through one end of the tube; a first discharge step which releases the second liquid sucked into the tube in the fifth suction step to discharge this second liquid into the second vessel; a second discharge step which releases the third liquid sucked into the tube in the fourth suction step to discharge this third liquid into the second vessel; a third discharge step which releases a part of the second liquid sucked into the tube in the third suction step to discharge this second liquid into the second vessel; a fourth discharge step which releases the second liquid remaining within the tube after the third discharge step to discharge this second liquid into the second vessel; a fifth discharge step which releases the first liquid sucked into the tube in the second suction step to discharge this first liquid into the second vessel; and a sixth discharge step which releases at least a part of the second liquid sucked into the tube in the first suction step to discharge this second liquid into the second vessel; wherein the second liquid is contained in the second vessel beforehand.