A leak detection system for use in a vacuum sterilization chamber, wherein a plurality of piping sections (60, 80, 120) are connected to the sterilization chamber through openings (78, 84, 124) along an inner surface of the sterilization chamber, the leak detection system comprising: an elongated flexible tubing dimensioned to connect to the openings (78, 84, 124) in the sterilization chamber; connectors connected to each end of the tubing, the connectors dimensioned to sealingly engage against the openings (78, 84, 124) in the sterilization chamber and to communicate a passage through the tubing with a piping section (60, 80, 120) connected to the sterilization chamber; a sensing device attached to the tubing operable to measure changes in pressure; valves (212A, 212B, 212C, 212D, 212E) disposed within the tubing operable to isolate one opening (78, 84, 124) in the steam sterilizer from another, said valves (212A, 212B, 212C, 212D, 212E) disposed relative to the sensing device to allow for selective isolation and sensing of pressure changes in one of the piping sections (60, 80, 120); wherein said connectors comprising inner surface connectors provided to connect the tubing to the openings (84, 124) within the sterilization chamber, the inner surface connectors comprising: an elongated tubular shaft, wherein an elongated passageway is extending axially through the elongated tubular shaft; an expansion assembly surrounding a portion of the elongated tubular shaft, the expansion assembly comprising: spaced apparat annular plates (254, 256) mounted to the elongated tubular shaft; and a resilient flexible expansion member disposed between the spaced apparat annular plates (254, 256), the resilient flexible expansion member being dimensioned to be inserted into one of the openings (84, 124) in the sterilization chamber and, when compressed between the spaced apparat annular plates (254, 256), to expand into sealing surface engagement with the inner surface defining the opening (84, 124). an elongated flexible tubing dimensioned to connect to the openings (78, 84, 124) in the sterilization chamber; connectors connected to each end of the tubing, the connectors dimensioned to sealingly engage against the openings (78, 84, 124) in the sterilization chamber and to communicate a passage through the tubing with a piping section (60, 80, 120) connected to the sterilization chamber; a sensing device attached to the tubing operable to measure changes in pressure; valves (212A, 212B, 212C, 212D, 212E) disposed within the tubing operable to isolate one opening (78, 84, 124) in the steam sterilizer from another, said valves (212A, 212B, 212C, 212D, 212E) disposed relative to the sensing device to allow for selective isolation and sensing of pressure changes in one of the piping sections (60, 80, 120); wherein said connectors comprising inner surface connectors provided to connect the tubing to the openings (84, 124) within the sterilization chamber, the inner surface connectors comprising: an elongated tubular shaft, wherein an elongated passageway is extending axially through the elongated tubular shaft; an expansion assembly surrounding a portion of the elongated tubular shaft, the expansion assembly comprising: spaced apparat annular plates (254, 256) mounted to the elongated tubular shaft; and a resilient flexible expansion member disposed between the spaced apparat annular plates (254, 256), the resilient flexible expansion member being dimensioned to be inserted into one of the openings (84, 124) in the sterilization chamber and, when compressed between the spaced apparat annular plates (254, 256), to expand into sealing surface engagement with the inner surface defining the opening (84, 124). an elongated tubular shaft, wherein an elongated passageway is extending axially through the elongated tubular shaft; an expansion assembly surrounding a portion of the elongated tubular shaft, the expansion assembly comprising: spaced apparat annular plates (254, 256) mounted to the elongated tubular shaft; and a resilient flexible expansion member disposed between the spaced apparat annular plates (254, 256), the resilient flexible expansion member being dimensioned to be inserted into one of the openings (84, 124) in the sterilization chamber and, when compressed between the spaced apparat annular plates (254, 256), to expand into sealing surface engagement with the inner surface defining the opening (84, 124). spaced apparat annular plates (254, 256) mounted to the elongated tubular shaft; and a resilient flexible expansion member disposed between the spaced apparat annular plates (254, 256), the resilient flexible expansion member being dimensioned to be inserted into one of the openings (84, 124) in the sterilization chamber and, when compressed between the spaced apparat annular plates (254, 256), to expand into sealing surface engagement with the inner surface defining the opening (84, 124).