An SPFS sensor chip, comprising a purification area and a sensor area arranged upstream and downstream, respectively, relative to each other along a flow direction in a channel for surface plasmon-field enhanced fluorescence spectroscopy [SPFS], wherein the sensor area comprises a transparent support, a metal membrane formed on one surface of the transparent support, a self-assembled monolayer formed on the surface of the metal membrane opposite to the transparent support, and a hydrophilic polymer layer formed on the surface of the SAM opposite to the metal membrane, and a protein for binding to a target substance immobilized on the surface of the hydrophilic polymer layer opposite to the SAM; wherein the purification area comprises a sample purification area and a labeled antibody purification area; wherein the sample purification area comprises a transparent support, a metal membrane formed on one surface of the transparent support, a self-assembled monolayer formed on the surface of the metal membrane opposite to the transparent support, and a hydrophilic polymer layer formed on the surface of the SAM opposite to the metal membrane, and does not comprise a protein for binding to the target substance; wherein the labeled antibody purification area comprises a transparent support, a metal membrane formed on one surface of the transparent support, a self-assembled monolayer formed on the surface of the metal membrane opposite to the transparent support, a hydrophilic polymer layer formed on the surface of the SAM opposite to the metal membrane, and a protein for binding to the target substance immobilized on the surface of the hydrophilic polymer layer opposite to the SAM; and wherein the channel is in such a form that the sample purification area and the labeled antibody purification area define respective branches and merge with each other to lead to the sensor area, and wherein the labeled antibody purification area and the sensor area are fabricated in the same manner, so that the protein for binding to the target substance in the labeled antibody purification area is the same as the protein for binding to the target substance in the sensor area. a purification area and a sensor area arranged upstream and downstream, respectively, relative to each other along a flow direction in a channel for surface plasmon-field enhanced fluorescence spectroscopy [SPFS], wherein the sensor area comprises a transparent support, a metal membrane formed on one surface of the transparent support, a self-assembled monolayer formed on the surface of the metal membrane opposite to the transparent support, and a hydrophilic polymer layer formed on the surface of the SAM opposite to the metal membrane, and a protein for binding to a target substance immobilized on the surface of the hydrophilic polymer layer opposite to the SAM; wherein the purification area comprises a sample purification area and a labeled antibody purification area; wherein the sample purification area comprises a transparent support, a metal membrane formed on one surface of the transparent support, a self-assembled monolayer formed on the surface of the metal membrane opposite to the transparent support, and a hydrophilic polymer layer formed on the surface of the SAM opposite to the metal membrane, and does not comprise a protein for binding to the target substance; wherein the labeled antibody purification area comprises a transparent support, a metal membrane formed on one surface of the transparent support, a self-assembled monolayer formed on the surface of the metal membrane opposite to the transparent support, a hydrophilic polymer layer formed on the surface of the SAM opposite to the metal membrane, and a protein for binding to the target substance immobilized on the surface of the hydrophilic polymer layer opposite to the SAM; and wherein the channel is in such a form that the sample purification area and the labeled antibody purification area define respective branches and merge with each other to lead to the sensor area, and wherein the labeled antibody purification area and the sensor area are fabricated in the same manner, so that the protein for binding to the target substance in the labeled antibody purification area is the same as the protein for binding to the target substance in the sensor area.