A surgical access assembly comprising: a housing; a tubular member extending from the housing; a valve assembly disposed in the housing, the valve assembly including: a guard assembly having a central orifice, and an instrument seal (160, 260) having a central hole (176, 266) aligned with the central orifice of the guard assembly, the instrument seal including a frame (170, 270) formed of a plurality of segments (172a-f, 272a-f), each segment having a top surface and a bottom surface and having a respective petal (162a-f, 262a-f) flexibly coupled to a side thereof by a living hinge, the instrument seal having an unfolded configuration defined by the petals (162a-f, 262a-f) extending away from a center of the frame (170, 270) and a folded configuration defined by the petals folded towards the central hole (176, 266) of the instrument seal wherein each petal at least partially overlaps an adjacent petal such that the petals interlock, characterized in that each petal (162a-f, 262a-f) is attached to the corresponding segment (172a-f, 272a-f) of the frame (170, 270) via the living hinge such that each petal and the corresponding living hinge define an acute angle with respect to either the top surface or the bottom surface of the segment (172a-f, 272a-f) of the frame (170, 270). a housing; a tubular member extending from the housing; a valve assembly disposed in the housing, the valve assembly including: a guard assembly having a central orifice, and an instrument seal (160, 260) having a central hole (176, 266) aligned with the central orifice of the guard assembly, the instrument seal including a frame (170, 270) formed of a plurality of segments (172a-f, 272a-f), each segment having a top surface and a bottom surface and having a respective petal (162a-f, 262a-f) flexibly coupled to a side thereof by a living hinge, the instrument seal having an unfolded configuration defined by the petals (162a-f, 262a-f) extending away from a center of the frame (170, 270) and a folded configuration defined by the petals folded towards the central hole (176, 266) of the instrument seal wherein each petal at least partially overlaps an adjacent petal such that the petals interlock, a guard assembly having a central orifice, and an instrument seal (160, 260) having a central hole (176, 266) aligned with the central orifice of the guard assembly, the instrument seal including a frame (170, 270) formed of a plurality of segments (172a-f, 272a-f), each segment having a top surface and a bottom surface and having a respective petal (162a-f, 262a-f) flexibly coupled to a side thereof by a living hinge, the instrument seal having an unfolded configuration defined by the petals (162a-f, 262a-f) extending away from a center of the frame (170, 270) and a folded configuration defined by the petals folded towards the central hole (176, 266) of the instrument seal wherein each petal at least partially overlaps an adjacent petal such that the petals interlock, characterized in that each petal (162a-f, 262a-f) is attached to the corresponding segment (172a-f, 272a-f) of the frame (170, 270) via the living hinge such that each petal and the corresponding living hinge define an acute angle with respect to either the top surface or the bottom surface of the segment (172a-f, 272a-f) of the frame (170, 270).