An imaging device (200, 501) for bright-field Fourier ptychographic imaging and fluorescence imaging, the imaging device (200, 501) comprising: a transparent well (22, 303, 403) for receiving a sample being imaged during operation of the imaging device (200, 501), the transparent well having a top end and a bottom end; a variable illumination source comprising an arrangement of discrete light elements at different locations configured to sequentially illuminate, at different illumination angles, at least a portion of the transparent well (22, 303, 403); a first fluorescence illumination source (220, 320, 420, 520) configured to provide excitation light of a first range of wavelengths to the transparent well (22, 303, 403), the first fluorescence illumination source (220, 320, 420, 520) located at a side of the transparent well (22, 303, 404) and oriented to provide the excitation light at an angle to a plane at a bottom surface of the transparent well (22, 303, 403); an optical system having a first objective and a second objective positioned oppositely; and an image sensor configured to receive light propagated through the optical system from the transparent well (22, 303, 403), the image sensor further configured to acquire image data for a sequence of uniquely-illuminated bright-field intensity images of the sample based at least in part on light passing through the transparent well while the transparent well is being illuminated by the sequential illumination at different illumination angles from the variable illumination source, the image sensor further configured to acquire image data for a first fluorescence image of the sample based at least in part on light emissions received from the sample in the transparent well in response to excitation light of the first range of wavelengths from the first fluorescence illumination source (220, 320, 420, 520), wherein the first objective is configured to collect light from the transparent well (22, 303, 403) and the second objective is configured to focus light to the image sensor. a transparent well (22, 303, 403) for receiving a sample being imaged during operation of the imaging device (200, 501), the transparent well having a top end and a bottom end; a variable illumination source comprising an arrangement of discrete light elements at different locations configured to sequentially illuminate, at different illumination angles, at least a portion of the transparent well (22, 303, 403); a first fluorescence illumination source (220, 320, 420, 520) configured to provide excitation light of a first range of wavelengths to the transparent well (22, 303, 403), the first fluorescence illumination source (220, 320, 420, 520) located at a side of the transparent well (22, 303, 404) and oriented to provide the excitation light at an angle to a plane at a bottom surface of the transparent well (22, 303, 403); an optical system having a first objective and a second objective positioned oppositely; and an image sensor configured to receive light propagated through the optical system from the transparent well (22, 303, 403), the image sensor further configured to acquire image data for a sequence of uniquely-illuminated bright-field intensity images of the sample based at least in part on light passing through the transparent well while the transparent well is being illuminated by the sequential illumination at different illumination angles from the variable illumination source, the image sensor further configured to acquire image data for a first fluorescence image of the sample based at least in part on light emissions received from the sample in the transparent well in response to excitation light of the first range of wavelengths from the first fluorescence illumination source (220, 320, 420, 520), wherein the first objective is configured to collect light from the transparent well (22, 303, 403) and the second objective is configured to focus light to the image sensor.