Method of microscopically recording images of samples extending in three-dimensions, comprising: - a first step, recording a first sectional view by scanning a sample in a sectional plane with an excitation light distribution which is focused by means of a microscope lens, wherein the excitation light distribution is corrected by means of an adjustable correction device according to initial adjustment parameters of an aberration correction function, and wherein, for each scanning position, fluorescence light emitted out of the sample is detected, or recording a first plurality of such sectional images, - a second step, evaluation of the first sectional image or of the first plurality of such sectional images, - a third step, setting new adjustment parameters of the aberration correction function on the basis of the evaluation in the second step, and - a fourth step, recording further image data by scanning a sample, which is the first sample or another sample, with the focused excitation light distribution, wherein the excitation light distribution is corrected by means of the adjustable correction device according to the new adjustment parameters of the aberration correction function, and wherein fluorescence light emitted out of the sample is detected for each scanning position, wherein the steps to are executed in the order of their naming, characterized in - that the sectional plane in which the sample is scanned with the focused excitation light distribution in the first step is parallel to the optical axis of the microscope lens such that the first sectional image is recorded parallel to the optical axis of the microscope lens, and - that the aberration correction function is a function of a z-position in the sample that varies along the optical axis of the microscope lens, wherein, at least when the new adjustment parameters of the aberration correction function are applied in the fourth step, the excitation light distribution is not corrected by means of the adjustable correction device for all z-positions in the sample in a same way. - a first step, recording a first sectional view by scanning a sample in a sectional plane with an excitation light distribution which is focused by means of a microscope lens, wherein the excitation light distribution is corrected by means of an adjustable correction device according to initial adjustment parameters of an aberration correction function, and wherein, for each scanning position, fluorescence light emitted out of the sample is detected, or recording a first plurality of such sectional images, - a second step, evaluation of the first sectional image or of the first plurality of such sectional images, - a third step, setting new adjustment parameters of the aberration correction function on the basis of the evaluation in the second step, and - a fourth step, recording further image data by scanning a sample, which is the first sample or another sample, with the focused excitation light distribution, wherein the excitation light distribution is corrected by means of the adjustable correction device according to the new adjustment parameters of the aberration correction function, and wherein fluorescence light emitted out of the sample is detected for each scanning position, wherein the steps to are executed in the order of their naming, characterized in - that the sectional plane in which the sample is scanned with the focused excitation light distribution in the first step is parallel to the optical axis of the microscope lens such that the first sectional image is recorded parallel to the optical axis of the microscope lens, and - that the aberration correction function is a function of a z-position in the sample that varies along the optical axis of the microscope lens, wherein, at least when the new adjustment parameters of the aberration correction function are applied in the fourth step, the excitation light distribution is not corrected by means of the adjustable correction device for all z-positions in the sample in a same way.