A method of controlling a process in a closed cell expansion system, the method comprising: providing a fluid conveyance assembly, wherein the fluid conveyance assembly comprises a bioreactor; engaging the fluid conveyance assembly; providing an air removal chamber, wherein the air removal chamber is mounted on the fluid conveyance assembly, and wherein the air removal chamber comprises a fluid containment chamber, the fluid containment chamber comprising: a fluid entrance aperture; a fluid exit aperture, wherein the fluid exit aperture is coupled to a fluid exit tube; and a vent aperture, wherein the vent aperture is located above the fluid entrance aperture and the fluid exit aperture; providing a sensor to detect the fluid level in the fluid containment chamber; providing a pump to pump a fluid through the fluid entrance aperture and into the fluid containment chamber of the air removal chamber; initiating the process, wherein the process comprises loading reagent from a reagent bag into the bioreactor until the reagent bag is empty, wherein the reagent is the fluid, comprising: operating the pump to pump the fluid through the fluid entrance aperture and into the fluid containment chamber, wherein the fluid reaches a fluid level in the fluid containment chamber; allowing the fluid to pass through the fluid exit aperture to enter the fluid exit tube; detecting the fluid level using the sensor; providing a stop condition, comprising: meeting the stop condition when the fluid level in the fluid containment chamber reaches a predetermined level as detected by the sensor; and when the fluid level in the fluid containment chamber is at the predetermined level, stopping the process based on meeting the stop condition. providing a fluid conveyance assembly, wherein the fluid conveyance assembly comprises a bioreactor; engaging the fluid conveyance assembly; providing an air removal chamber, wherein the air removal chamber is mounted on the fluid conveyance assembly, and wherein the air removal chamber comprises a fluid containment chamber, the fluid containment chamber comprising: a fluid entrance aperture; a fluid exit aperture, wherein the fluid exit aperture is coupled to a fluid exit tube; and a vent aperture, wherein the vent aperture is located above the fluid entrance aperture and the fluid exit aperture; a fluid entrance aperture; a fluid exit aperture, wherein the fluid exit aperture is coupled to a fluid exit tube; and a vent aperture, wherein the vent aperture is located above the fluid entrance aperture and the fluid exit aperture; providing a sensor to detect the fluid level in the fluid containment chamber; providing a pump to pump a fluid through the fluid entrance aperture and into the fluid containment chamber of the air removal chamber; initiating the process, wherein the process comprises loading reagent from a reagent bag into the bioreactor until the reagent bag is empty, wherein the reagent is the fluid, comprising: operating the pump to pump the fluid through the fluid entrance aperture and into the fluid containment chamber, wherein the fluid reaches a fluid level in the fluid containment chamber; allowing the fluid to pass through the fluid exit aperture to enter the fluid exit tube; detecting the fluid level using the sensor; operating the pump to pump the fluid through the fluid entrance aperture and into the fluid containment chamber, wherein the fluid reaches a fluid level in the fluid containment chamber; allowing the fluid to pass through the fluid exit aperture to enter the fluid exit tube; detecting the fluid level using the sensor; providing a stop condition, comprising: meeting the stop condition when the fluid level in the fluid containment chamber reaches a predetermined level as detected by the sensor; and when the fluid level in the fluid containment chamber is at the predetermined level, stopping the process based on meeting the stop condition.