A Raman spectroscopic detection method for detecting a sample in a vessel, comprising the steps of: (a) measuring a Raman spectrum of the vessel to obtain a first Raman spectroscopic signal; (b) measuring a Raman spectrum of the sample through the vessel to obtain a second Raman spectroscopic signal; (c) removing an interference caused by the Raman spectrum of the vessel from the second Raman spectroscopic signal on basis of the first Raman spectroscopic signal to obtain a third Raman spectroscopic signal of the sample itself; and (d) identifying the sample on basis of the third Raman spectroscopic signal, characterized in that the step (c) comprises: extracting a plurality of characterizing intensity values and characterizing positions where the characterizing intensity values are present from the first Raman spectroscopic signal; extracting, from the second Raman spectroscopic signal, a plurality of characterizing intensity values which are present in positions corresponding to the characterizing positions in the first Raman spectroscopic signal; calculating a subtraction coefficient on basis of the plurality of characterizing intensity values in the first Raman spectroscopic signal and the plurality of characterizing intensity values extracted from the second Raman spectroscopic signal; and subtracting the product of the first Raman spectroscopic signal and the subtraction coefficient from the second Raman spectroscopic signal to obtain the third Raman spectroscopic signal, wherein the step comprises the following steps of: dividing the plurality of characterizing intensity values extracted from the second Raman spectroscopic signal by the plurality of characterizing intensity values in the first Raman spectroscopic signal to obtain a plurality of quotients respectively, the plurality of characterizing intensity values extracted from the second Raman spectroscopic signal corresponding to the plurality of characterizing intensity values in the first Raman spectroscopic signal in positions; and selecting the minimum of the quotients as the subtraction coefficient. (a) measuring a Raman spectrum of the vessel to obtain a first Raman spectroscopic signal; (b) measuring a Raman spectrum of the sample through the vessel to obtain a second Raman spectroscopic signal; (c) removing an interference caused by the Raman spectrum of the vessel from the second Raman spectroscopic signal on basis of the first Raman spectroscopic signal to obtain a third Raman spectroscopic signal of the sample itself; and (d) identifying the sample on basis of the third Raman spectroscopic signal, extracting a plurality of characterizing intensity values and characterizing positions where the characterizing intensity values are present from the first Raman spectroscopic signal; extracting, from the second Raman spectroscopic signal, a plurality of characterizing intensity values which are present in positions corresponding to the characterizing positions in the first Raman spectroscopic signal; calculating a subtraction coefficient on basis of the plurality of characterizing intensity values in the first Raman spectroscopic signal and the plurality of characterizing intensity values extracted from the second Raman spectroscopic signal; and subtracting the product of the first Raman spectroscopic signal and the subtraction coefficient from the second Raman spectroscopic signal to obtain the third Raman spectroscopic signal, dividing the plurality of characterizing intensity values extracted from the second Raman spectroscopic signal by the plurality of characterizing intensity values in the first Raman spectroscopic signal to obtain a plurality of quotients respectively, the plurality of characterizing intensity values extracted from the second Raman spectroscopic signal corresponding to the plurality of characterizing intensity values in the first Raman spectroscopic signal in positions; and selecting the minimum of the quotients as the subtraction coefficient.