A fluorescence imaging system for imaging an object, comprising: a light source assembly comprising: a white light provider (212, 252, 262, 512) arranged for emitting white light; an excitation light provider (214, 254a, 254b, 264a, 264b, 514) arranged for emitting excitation light in a plurality of excitation wavebands for causing the object to emit fluorescent light; at least one image sensor (223, 540, 1115) arranged for receiving reflected white light and emitted fluorescent light from the object; and an optical assembly (226, 530) located in the optical path between the object and the image sensor, comprising: a first optics region arranged for projecting the reflected white light as a white light image onto the image sensor; and a second optics region (1200a, 1200b, 1200c, 1200d) arranged for demagnifying the fluorescent light for producing fluorescence images that are smaller in size than the white light image, spectrally separating the fluorescent light into multiple fluorescent light wavebands, and projecting each of the multiple fluorescent light wavebands as different fluorescence images onto different portions of the image sensor. a white light provider (212, 252, 262, 512) arranged for emitting white light; an excitation light provider (214, 254a, 254b, 264a, 264b, 514) arranged for emitting excitation light in a plurality of excitation wavebands for causing the object to emit fluorescent light; at least one image sensor (223, 540, 1115) arranged for receiving reflected white light and emitted fluorescent light from the object; and an optical assembly (226, 530) located in the optical path between the object and the image sensor, comprising: a first optics region arranged for projecting the reflected white light as a white light image onto the image sensor; and a second optics region (1200a, 1200b, 1200c, 1200d) arranged for demagnifying the fluorescent light for producing fluorescence images that are smaller in size than the white light image, spectrally separating the fluorescent light into multiple fluorescent light wavebands, and projecting each of the multiple fluorescent light wavebands as different fluorescence images onto different portions of the image sensor. at least one image sensor (223, 540, 1115) arranged for receiving reflected white light and emitted fluorescent light from the object; and an optical assembly (226, 530) located in the optical path between the object and the image sensor, comprising: a first optics region arranged for projecting the reflected white light as a white light image onto the image sensor; and a second optics region (1200a, 1200b, 1200c, 1200d) arranged for demagnifying the fluorescent light for producing fluorescence images that are smaller in size than the white light image, spectrally separating the fluorescent light into multiple fluorescent light wavebands, and projecting each of the multiple fluorescent light wavebands as different fluorescence images onto different portions of the image sensor. a first optics region arranged for projecting the reflected white light as a white light image onto the image sensor; and a second optics region (1200a, 1200b, 1200c, 1200d) arranged for demagnifying the fluorescent light for producing fluorescence images that are smaller in size than the white light image, spectrally separating the fluorescent light into multiple fluorescent light wavebands, and projecting each of the multiple fluorescent light wavebands as different fluorescence images onto different portions of the image sensor.