A cartridge for a fluid sampling device for collecting a fluid sample from a test subject, including: a reservoir; a first conduit fluidly connecting the reservoir to a first fluid fitting configured to fluidly connect the cartridge to a tubing having a distal end inserted into the subject; a second conduit fluidly connecting the reservoir to a second fluid fitting configured to connect the cartridge to a sample collection component; a third conduit fluidly connecting the reservoir to a third fluid fitting configured to connect the cartridge to a sterile fluid supply; and a fourth conduit fluidly connecting the reservoir to a fourth fluid fitting configured to connect the cartridge to a pump, the pump being moveable in a first direction to draw fluid into the pump and a second direction to expel fluid from the pump; wherein the fluid conduits (40, 42, 46, 48) are configured such that when the cartridge is operably connected to the fluid sampling device, a first valve of the fluid sampling device controls fluid flow in the first fluid conduit, a second valve of the fluid sampling device controls fluid flow in the second fluid conduit, and a third valve of the fluid sampling device controls fluid flow in the third fluid conduit; and a frame which is configured to be removably attached to the fluid sampling device, wherein the reservoir and fluid conduits(40, 42, 46, 48) are secured to the frame, wherein the cartridge includes at least one window (64, 66, 68) configured to allow the first valve of the fluid sampling device to control fluid flow in the first fluid conduit by applying compressive force to the first fluid conduit, the second valve of the fluid sampling device to control fluid flow in the second fluid conduit by applying compressive force to the second fluid conduit, and the third valve of the fluid sampling device to control fluid flow in the third fluid conduit by applying compressive force to the third fluid conduit. a reservoir; a first conduit fluidly connecting the reservoir to a first fluid fitting configured to fluidly connect the cartridge to a tubing having a distal end inserted into the subject; a second conduit fluidly connecting the reservoir to a second fluid fitting configured to connect the cartridge to a sample collection component; a third conduit fluidly connecting the reservoir to a third fluid fitting configured to connect the cartridge to a sterile fluid supply; and a fourth conduit fluidly connecting the reservoir to a fourth fluid fitting configured to connect the cartridge to a pump, the pump being moveable in a first direction to draw fluid into the pump and a second direction to expel fluid from the pump; wherein the fluid conduits (40, 42, 46, 48) are configured such that when the cartridge is operably connected to the fluid sampling device, a first valve of the fluid sampling device controls fluid flow in the first fluid conduit, a second valve of the fluid sampling device controls fluid flow in the second fluid conduit, and a third valve of the fluid sampling device controls fluid flow in the third fluid conduit; and a frame which is configured to be removably attached to the fluid sampling device, wherein the reservoir and fluid conduits(40, 42, 46, 48) are secured to the frame, wherein the cartridge includes at least one window (64, 66, 68) configured to allow the first valve of the fluid sampling device to control fluid flow in the first fluid conduit by applying compressive force to the first fluid conduit, the second valve of the fluid sampling device to control fluid flow in the second fluid conduit by applying compressive force to the second fluid conduit, and the third valve of the fluid sampling device to control fluid flow in the third fluid conduit by applying compressive force to the third fluid conduit.