A system for treating at least one item, comprising: a first unit comprising a disinfectant generator, wherein the disinfectant generator comprises: a free radical generator, wherein the free radical generator is configured to generate free radicals and the free radical generator is a cold plasma generator that generates ozone; a reservoir of disinfectant media; a vaporizer unit in fluidic communication with the reservoir of disinfectant media, wherein the vaporizer unit is configured to generate a vapor of said disinfectant media; a gas distribution unit, wherein the gas distribution unit conveys a gas from at least one outlet of the gas distribution unit to an inlet of the free radical generator and to the reservoir of disinfectant media or the vaporizer unit, wherein the disinfectant generator is configured to generate an effluent capable of disinfection or sanitization of the at least one item; a second unit comprising: a chamber for containing an item or items to be treated, wherein the chamber is configured to form a sealed and enclosed area which can receive the at least one item, and at least one conduit in fluidic communication with the first unit and the second unit, wherein the conduit is configured to convey the disinfecting effluent from the first unit to the second unit; characterized in that the system is configured to operate at a humidity within an interior of the chamber of between about 20% and 90% relative humidity, and the system is configured to operate at a temperature in the range of 15.6 °C to 26. 7 °C during disinfection or sterilization. a free radical generator, wherein the free radical generator is configured to generate free radicals and the free radical generator is a cold plasma generator that generates ozone; a reservoir of disinfectant media; a vaporizer unit in fluidic communication with the reservoir of disinfectant media, wherein the vaporizer unit is configured to generate a vapor of said disinfectant media; a gas distribution unit, wherein the gas distribution unit conveys a gas from at least one outlet of the gas distribution unit to an inlet of the free radical generator and to the reservoir of disinfectant media or the vaporizer unit, wherein the disinfectant generator is configured to generate an effluent capable of disinfection or sanitization of the at least one item; a second unit comprising: a chamber for containing an item or items to be treated, wherein the chamber is configured to form a sealed and enclosed area which can receive the at least one item, and at least one conduit in fluidic communication with the first unit and the second unit, wherein the conduit is configured to convey the disinfecting effluent from the first unit to the second unit; characterized in that the system is configured to operate at a humidity within an interior of the chamber of between about 20% and 90% relative humidity, and the system is configured to operate at a temperature in the range of 15.6 °C to 26. 7 °C during disinfection or sterilization. a chamber for containing an item or items to be treated, wherein the chamber is configured to form a sealed and enclosed area which can receive the at least one item, and at least one conduit in fluidic communication with the first unit and the second unit, wherein the conduit is configured to convey the disinfecting effluent from the first unit to the second unit; characterized in that the system is configured to operate at a humidity within an interior of the chamber of between about 20% and 90% relative humidity, and the system is configured to operate at a temperature in the range of 15.6 °C to 26. 7 °C during disinfection or sterilization.