A lens free imaging device for imaging a sample, the lens free imaging device comprising a radiation guiding structure comprising a waveguide comprising a first surface parallel with a second surface, and an imaging region, wherein Z the radiation guiding structure is adapted for receiving an incoming radiation wave thus obtaining a confined radiation wave in the radiation guiding structure by total internal reflection at the first and second surface (115, 117) resulting in an overall propagation of the confined radiation wave in a direction parallel with both surfaces, - wherein the waveguide comprises a diffracting element adapted for redirecting the confined radiation wave in a first direction resulting in a first radiation wave, and in a second direction resulting in a second radiation wave, - or wherein the waveguide comprises a first diffracting element and a second diffracting element, wherein the first diffracting element is adapted for diffracting the confined radiation wave in the first direction resulting in the first radiation wave, and wherein the second diffracting element is adapted for diffracting the confined radiation wave in the second direction resulting in the second radiation wave, wherein the radiation guiding structure is configured for directing the first radiation wave towards the first surface and out of the radiation guiding structure to a sample measurement region for allowing interaction between the first radiation wave and the sample when positioned in the sample measurement region, and for directing the second radiation wave towards the second surface towards the imaging region, the radiation guiding structure, the sample measurement region and the imaging region furthermore being configured so that a scattered first radiation wave, scattered by the sample is at least partly captured in the imaging region and can be combined with the second radiation wave for forming an image. - wherein the waveguide comprises a diffracting element adapted for redirecting the confined radiation wave in a first direction resulting in a first radiation wave, and in a second direction resulting in a second radiation wave, - or wherein the waveguide comprises a first diffracting element and a second diffracting element, wherein the first diffracting element is adapted for diffracting the confined radiation wave in the first direction resulting in the first radiation wave, and wherein the second diffracting element is adapted for diffracting the confined radiation wave in the second direction resulting in the second radiation wave, wherein the radiation guiding structure is configured for directing the first radiation wave towards the first surface and out of the radiation guiding structure to a sample measurement region for allowing interaction between the first radiation wave and the sample when positioned in the sample measurement region, and for directing the second radiation wave towards the second surface towards the imaging region, the radiation guiding structure, the sample measurement region and the imaging region furthermore being configured so that a scattered first radiation wave, scattered by the sample is at least partly captured in the imaging region and can be combined with the second radiation wave for forming an image.