A separation system to separate a multiple component material into at least two fractions, comprising: i) a separation container defining a volume within an interior wall, a first end wall, and a second end wall; and ii) a buoy separation system in the volume operable to move to a selected interface of the multiple component material under the influence of a force, the buoy separation system including: - a first buoy portion having a passage between a first side and a second side of the first buoy portion; - a second buoy portion at a selected distance from the second side of the first buoy portion, wherein the selected distance defines a collection volume between the second side of the first buoy portion and the second buoy portion; and - a valve assembly having a sealing portion and a flexible portion, wherein the flexible portion holds the sealing portion against the first buoy portion to seal the passage in the first buoy portion to separate the collection volume from a volume exterior to the collection volume on the first side of the first buoy portion; wherein the sealing portion and the flexible portion are a separate and distinct sealing member and flexible valve actuation member moveable relative to one another; wherein each of the sealing member and the flexible valve actuation member have an inner wall defining a passage of the valve assembly which is fitted around a central tube extending from the first buoy portion into the collection volume; wherein at least a portion of the flexible valve actuation member is fixed relative to the first buoy portion; wherein the valve assembly is operable to be unsealed from the first buoy portion upon the application of a force to allow a selected fraction to move into the collection volume; and wherein the flexible valve actuation member is formed of a resilient material and is configured to provide a biasing or closing force on the sealing member against the first buoy portion. i) a separation container defining a volume within an interior wall, a first end wall, and a second end wall; and ii) a buoy separation system in the volume operable to move to a selected interface of the multiple component material under the influence of a force, the buoy separation system including: - a first buoy portion having a passage between a first side and a second side of the first buoy portion; - a second buoy portion at a selected distance from the second side of the first buoy portion, wherein the selected distance defines a collection volume between the second side of the first buoy portion and the second buoy portion; and - a valve assembly having a sealing portion and a flexible portion, wherein the flexible portion holds the sealing portion against the first buoy portion to seal the passage in the first buoy portion to separate the collection volume from a volume exterior to the collection volume on the first side of the first buoy portion; - a first buoy portion having a passage between a first side and a second side of the first buoy portion; - a second buoy portion at a selected distance from the second side of the first buoy portion, wherein the selected distance defines a collection volume between the second side of the first buoy portion and the second buoy portion; and - a valve assembly having a sealing portion and a flexible portion, wherein the flexible portion holds the sealing portion against the first buoy portion to seal the passage in the first buoy portion to separate the collection volume from a volume exterior to the collection volume on the first side of the first buoy portion;