An apparatus for sterilization of an article, the article having a first end, a second end and a lumen extending between the first end and the second end, the apparatus comprising: a first chamber having: an outlet configured to be fluidly connected to a pump for adjusting an internal pressure in the first chamber; and an inlet configured to be fluidly connected to a sterilant source for supplying sterilant to the first chamber; a container configured to house the article, the container being sized and shaped to be receivable in the first chamber, the container having: a container inlet arranged to allow ingress of the sterilant from the first chamber, and a container outlet comprising a container connector port, the container connector port being configured to fluidly connect to the lumen at the second end of the article when the article is housed in the container; a second chamber configured to be fluidly connected to the container outlet when the container is received in the first chamber, the second chamber comprising a chamber connector port configured to fluidly connect to the container connector port to form a fluid path extending from the first chamber to the second chamber through the lumen of the article when the article is housed in the container and the container is received in the first chamber; and a valve between the second chamber and the container outlet, the valve being configurable between an open position and a closed position for fluidly connecting and isolating the first and second chambers respectively. a first chamber having: an outlet configured to be fluidly connected to a pump for adjusting an internal pressure in the first chamber; and an inlet configured to be fluidly connected to a sterilant source for supplying sterilant to the first chamber; an outlet configured to be fluidly connected to a pump for adjusting an internal pressure in the first chamber; and an inlet configured to be fluidly connected to a sterilant source for supplying sterilant to the first chamber; a container configured to house the article, the container being sized and shaped to be receivable in the first chamber, the container having: a container inlet arranged to allow ingress of the sterilant from the first chamber, and a container outlet comprising a container connector port, the container connector port being configured to fluidly connect to the lumen at the second end of the article when the article is housed in the container; a container inlet arranged to allow ingress of the sterilant from the first chamber, and a container outlet comprising a container connector port, the container connector port being configured to fluidly connect to the lumen at the second end of the article when the article is housed in the container; a second chamber configured to be fluidly connected to the container outlet when the container is received in the first chamber, the second chamber comprising a chamber connector port configured to fluidly connect to the container connector port to form a fluid path extending from the first chamber to the second chamber through the lumen of the article when the article is housed in the container and the container is received in the first chamber; and a valve between the second chamber and the container outlet, the valve being configurable between an open position and a closed position for fluidly connecting and isolating the first and second chambers respectively.