A method for detecting fluorescence emitted from a sample labeled with a plurality of predetermined fluorochromes using a microscopy system comprising a filter arrangement and a light source arrangement, wherein the method comprises the steps of: - selecting at least four different fluorochromes configured to emit light within the visible and the far red, the at least four different fluorochromes including a first and a second fluorochrome forming a pair of fluorochromes, the excitation spectra of the first and the second fluorochrome being partly overlapping and the emission spectra also being partly overlapping; - selecting bandpass excitation wavelength intervals for the at least four different fluorochromes, wherein the bandpass excitation wavelength interval for the second fluorochrome is selected such that the excitation of the first fluorochrome is reduced; - selecting emission wavelength intervals for the at least four different fluorochromes, - configuring the filter arrangement to comprise a plurality of microscope filter sets in turn configured to selectively allow light to pass through within the selected bandpass emission wavelength intervals matching the emission wavelength intervals of the at least four different fluorochromes, wherein the bandpass emission wavelength interval used for the first fluorochrome is selected to reduce light emission bleedthrough from the second fluorochrome; - sequentially emitting light within the selected bandpass excitation wavelength intervals using the light source arrangement in combination with the filter arrangement comprising the filter sets; and - detecting light emitted from the sample transmitted through the filter arrangement, wherein: - the bandpass excitation and bandpass emission wavelength intervals are selected to allow multicolor analysis when a single sample is labeled with the at least four fluorochromes, whereby, - the pair of fluorochromes are selected as a Cy3 analog fluorochrome forming the first fluorochrome and a 594 analog fluorochrome forming the second fluorochrome, - two further fluorochromes are selected to be a 488 analog fluorochrome and a 647 analog fluorochrome, - the bandpass excitation wavelength interval for the Cy3 analog fluorochrome is 535 - 555 nm, - the bandpass emission wavelength interval for the Cy3 analog fluorochrome is 555 - 590 nm, - the bandpass excitation wavelength interval for the 594 analog fluorochrome is 585 - 615 nm, - the bandpass emission wavelength interval for the 594 analog fluorochrome is 605 - 655 nm, - the bandpass excitation wavelength interval for the 488 analog fluorochrome is 480 - 520 nm, - the bandpass emission wavelength interval for the 488 analog fluorochrome is 500 - 535 nm, - the bandpass excitation wavelength interval for the 647 analog fluorochrome is 630 - 665 nm, and - the bandpass emission wavelength interval for the 647 analog fluorochrome has a lower end of 650 nm. - selecting at least four different fluorochromes configured to emit light within the visible and the far red, the at least four different fluorochromes including a first and a second fluorochrome forming a pair of fluorochromes, the excitation spectra of the first and the second fluorochrome being partly overlapping and the emission spectra also being partly overlapping; - selecting bandpass excitation wavelength intervals for the at least four different fluorochromes, wherein the bandpass excitation wavelength interval for the second fluorochrome is selected such that the excitation of the first fluorochrome is reduced; - selecting emission wavelength intervals for the at least four different fluorochromes, - configuring the filter arrangement to comprise a plurality of microscope filter sets in turn configured to selectively allow light to pass through within the selected bandpass emission wavelength intervals matching the emission wavelength intervals of the at least four different fluorochromes, wherein the bandpass emission wavelength interval used for the first fluorochrome is selected to reduce light emission bleedthrough from the second fluorochrome; - sequentially emitting light within the selected bandpass excitation wavelength intervals using the light source arrangement in combination with the filter arrangement comprising the filter sets; and - detecting light emitted from the sample transmitted through the filter arrangement, - the bandpass excitation and bandpass emission wavelength intervals are selected to allow multicolor analysis when a single sample is labeled with the at least four fluorochromes, whereby, - the pair of fluorochromes are selected as a Cy3 analog fluorochrome forming the first fluorochrome and a 594 analog fluorochrome forming the second fluorochrome, - two further fluorochromes are selected to be a 488 analog fluorochrome and a 647 analog fluorochrome, - the bandpass excitation wavelength interval for the Cy3 analog fluorochrome is 535 - 555 nm, - the bandpass emission wavelength interval for the Cy3 analog fluorochrome is 555 - 590 nm, - the bandpass excitation wavelength interval for the 594 analog fluorochrome is 585 - 615 nm, - the bandpass emission wavelength interval for the 594 analog fluorochrome is 605 - 655 nm, - the bandpass excitation wavelength interval for the 488 analog fluorochrome is 480 - 520 nm, - the bandpass emission wavelength interval for the 488 analog fluorochrome is 500 - 535 nm, - the bandpass excitation wavelength interval for the 647 analog fluorochrome is 630 - 665 nm, and - the bandpass emission wavelength interval for the 647 analog fluorochrome has a lower end of 650 nm.