A system for irreversibly sealing a nucleic acid amplification reaction vessel (210, 870), comprising: a base, comprising: a top surface of the base and a bottom surface of the base, and an opening in the top surface of the base defining a passage that extends from the opening in the top surface of the base to an opening in the bottom surface of the base; a nucleic acid amplification reaction vessel (210, 870) which is seated within the passage and protrudes through the opening in the bottom surface of the base, comprising: a receptacle portion distal to the bottom surface of the base for holding a nucleic acid amplification reaction mixture, walls of the nucleic acid amplification reaction vessel, comprising an inner surface, wherein the walls of the nucleic acid amplification reaction vessel extend from the receptacle portion of the nucleic acid amplification reaction vessel towards the bottom surface of the base, and a collection cap (240, 875), characterized in that the collection cap comprises: a sample collecting tip, a top portion of the collection cap distal to the sample collecting tip of the collection cap, and an outer surface of the collection cap between the sample collecting tip of the collection cap and the top portion of the collection cap, wherein when the collection cap is inserted through the passage and into the nucleic acid amplification reaction vessel, the outer surface of the collection cap contacts the inner surface of the walls of the nucleic acid amplification reaction vessel, and when sealed, the top portion of the collection cap is recessed beneath the opening in the top surface of the base so that the collection cap is inaccessible. a base, comprising: a top surface of the base and a bottom surface of the base, and an opening in the top surface of the base defining a passage that extends from the opening in the top surface of the base to an opening in the bottom surface of the base; a top surface of the base and a bottom surface of the base, and an opening in the top surface of the base defining a passage that extends from the opening in the top surface of the base to an opening in the bottom surface of the base; a nucleic acid amplification reaction vessel (210, 870) which is seated within the passage and protrudes through the opening in the bottom surface of the base, comprising: a receptacle portion distal to the bottom surface of the base for holding a nucleic acid amplification reaction mixture, walls of the nucleic acid amplification reaction vessel, comprising an inner surface, wherein the walls of the nucleic acid amplification reaction vessel extend from the receptacle portion of the nucleic acid amplification reaction vessel towards the bottom surface of the base, and a receptacle portion distal to the bottom surface of the base for holding a nucleic acid amplification reaction mixture, walls of the nucleic acid amplification reaction vessel, comprising an inner surface, wherein the walls of the nucleic acid amplification reaction vessel extend from the receptacle portion of the nucleic acid amplification reaction vessel towards the bottom surface of the base, and a collection cap (240, 875), characterized in that the collection cap comprises: a sample collecting tip, a top portion of the collection cap distal to the sample collecting tip of the collection cap, and an outer surface of the collection cap between the sample collecting tip of the collection cap and the top portion of the collection cap, a sample collecting tip, a top portion of the collection cap distal to the sample collecting tip of the collection cap, and an outer surface of the collection cap between the sample collecting tip of the collection cap and the top portion of the collection cap, wherein when the collection cap is inserted through the passage and into the nucleic acid amplification reaction vessel, the outer surface of the collection cap contacts the inner surface of the walls of the nucleic acid amplification reaction vessel, and when sealed, the top portion of the collection cap is recessed beneath the opening in the top surface of the base so that the collection cap is inaccessible.