An apparatus, comprising: a housing supporting or encompassing a blood separation well for collection of a blood sample, the blood separation well having a recess intersecting a surface of the housing, wherein the surface of the recess is defined further as a capillary surface having at least one microchannel; a separation membrane positioned on the surface of the recess for filtration of the blood sample to provide filtered plasma; a plasma collection vessel configured to collect filtered plasma; a first channel connecting the blood separation well to the plasma collection vessel proximate to the surface of the recess; and, a second channel connecting the plasma collection vessel to a first outlet port, and a filter positioned within the recess (30, the second side of the filter being in proximity to or in contact with the separation membrane, wherein the filter is formed of one or more layers, a seal is positioned on a distal end of the plasma collection vessel, wherein the seal is formed of a pierceable material; the second channel is positioned above the first channel and in proximity to the distal end of the plasma collection vessel such that, when a negative pressure force is applied to the first outlet port of the second channel, filtered plasma is drawn into the collection vessel, wherein the first channel, the plasma collection vessel, and the second channel are configured to convey the negative pressure force applied to the first outlet port to the surface of the recess, characterized in that an adhesive member is disposed within the recess and positioned on the capillary surface, with the separation membrane and the filter positioned thereon, respectively, wherein the filter, the separation membrane and the adhesive member are stacked on the capillary surface and wherein the filter and the separation membrane are pressure fit within the recess. a housing supporting or encompassing a blood separation well for collection of a blood sample, the blood separation well having a recess intersecting a surface of the housing, wherein the surface of the recess is defined further as a capillary surface having at least one microchannel; a separation membrane positioned on the surface of the recess for filtration of the blood sample to provide filtered plasma; a plasma collection vessel configured to collect filtered plasma; a first channel connecting the blood separation well to the plasma collection vessel proximate to the surface of the recess; and, a second channel connecting the plasma collection vessel to a first outlet port, and a filter positioned within the recess (30, the second side of the filter being in proximity to or in contact with the separation membrane, wherein the filter is formed of one or more layers, a seal is positioned on a distal end of the plasma collection vessel, wherein the seal is formed of a pierceable material; the second channel is positioned above the first channel and in proximity to the distal end of the plasma collection vessel such that, when a negative pressure force is applied to the first outlet port of the second channel, filtered plasma is drawn into the collection vessel, wherein the first channel, the plasma collection vessel, and the second channel are configured to convey the negative pressure force applied to the first outlet port to the surface of the recess, characterized in that an adhesive member is disposed within the recess and positioned on the capillary surface, with the separation membrane and the filter positioned thereon, respectively, wherein the filter, the separation membrane and the adhesive member are stacked on the capillary surface and wherein the filter and the separation membrane are pressure fit within the recess. the second channel is positioned above the first channel and in proximity to the distal end of the plasma collection vessel such that, when a negative pressure force is applied to the first outlet port of the second channel, filtered plasma is drawn into the collection vessel, wherein the first channel, the plasma collection vessel, and the second channel are configured to convey the negative pressure force applied to the first outlet port to the surface of the recess, characterized in that an adhesive member is disposed within the recess and positioned on the capillary surface, with the separation membrane and the filter positioned thereon, respectively, wherein the filter, the separation membrane and the adhesive member are stacked on the capillary surface and wherein the filter and the separation membrane are pressure fit within the recess.