A microfluidic blood type detection chip, the chip comprises a chip body, and the chip body comprises: a forward typing blood type identification area, which is provided with a first sample injection chamber, a quantitative mixing chamber, and a forward typing reaction chamber; characterized in that, a reverse typing blood type identification area, which is provided with a second sample injection chamber, a quantitative separation chamber, and a reverse typing reaction chamber; wherein the quantitative separation chamber is configured to stratify plasma sample and blood cell sample from the sample to be detected; vent holes communicated with the forward typing reaction chamber and the reverse typing reaction chamber respectively; the first sample injection chamber and the second sample injection chamber are used for injecting a sample to be detected, and are respectively connected with the quantitative mixing chamber and the quantitative separation chamber through a microfluidic channel, the reaction sample enters the quantitative mixing chamber and the quantitative separation chamber respectively from the first sample injection chamber and the second sample injection chamber; a diluent is preset in the first sample injection chamber, the diluent is configured to enter the quantitative mixing chamber through the first sample injection chamber; a diluent is preset in the second sample injection chamber, the diluent is configured to enter the quantitative separation chamber through the second sample injection chamber; the forward typing reaction chamber is configured for, when the reaction sample and the diluent in the first sample injection chamber are mixed in the quantitative mixing chamber, the blood cell sample enters the forward typing reaction chamber through a microfluidic channel to react with reaction reagent therein for detection; the reverse typing reaction chamber is configured for, the plasma sample separated from the quantitative separation chamber enters the reverse typing reaction chamber through a microfluidic channel to react with reaction reagent therein for detection; a hematocrit detection tube groove, which is connected to the quantitative separation chamber and is located on the side of the quantitative separation chamber close to the forward typing blood type identification area. a reverse typing blood type identification area, which is provided with a second sample injection chamber, a quantitative separation chamber, and a reverse typing reaction chamber; wherein the quantitative separation chamber is configured to stratify plasma sample and blood cell sample from the sample to be detected; vent holes communicated with the forward typing reaction chamber and the reverse typing reaction chamber respectively; the first sample injection chamber and the second sample injection chamber are used for injecting a sample to be detected, and are respectively connected with the quantitative mixing chamber and the quantitative separation chamber through a microfluidic channel, the reaction sample enters the quantitative mixing chamber and the quantitative separation chamber respectively from the first sample injection chamber and the second sample injection chamber; a diluent is preset in the first sample injection chamber, the diluent is configured to enter the quantitative mixing chamber through the first sample injection chamber; a diluent is preset in the second sample injection chamber, the diluent is configured to enter the quantitative separation chamber through the second sample injection chamber; the forward typing reaction chamber is configured for, when the reaction sample and the diluent in the first sample injection chamber are mixed in the quantitative mixing chamber, the blood cell sample enters the forward typing reaction chamber through a microfluidic channel to react with reaction reagent therein for detection; the reverse typing reaction chamber is configured for, the plasma sample separated from the quantitative separation chamber enters the reverse typing reaction chamber through a microfluidic channel to react with reaction reagent therein for detection; a hematocrit detection tube groove, which is connected to the quantitative separation chamber and is located on the side of the quantitative separation chamber close to the forward typing blood type identification area.