Disrupting device of biologic substances comprising: - at least one external hollow body defining at least one internal chamber; - at least one fixed disrupting grid housed inside said internal chamber to define at least one loading chamber of biologic substances to be disrupted and at least one collecting chamber of disrupted biologic substances, said fixed disrupting grid being composed of a case with passages, of a substantially axial-symmetrical shape with respect to at least one rotor, to delimit said loading chamber of biologic substances to be disrupted around a rotation axis of said rotor and said collecting chamber of disrupted biologic substances between said fixed disrupting grid and said external hollow body; and - said at least one rotor rotating inside said internal chamber to take said biologic substances in contact with said fixed disrupting grid and to cooperate with said fixed disrupting grid to allow a disruption and a passage from said loading chamber to said collecting chamber of said disrupted biologic substances through said fixed disrupting grid; said rotor comprises at least one blade, radially projecting with respect to said rotation axis of said rotor, said blade extending substantially for the whole height of said fixed disrupting grid, said blade being designed to take said biologic substances in contact with said fixed disrupting grid, characterized in that said blade comprising at least one convex surface with tapered end, in contact with said biologic substances to be taken in contact with said fixed disrupting grid; and in that said fixed disrupting grid comprises a distribution of holed micro-cells, said holed micro-cells being equipped with cutting edges around said rotation axis of said rotor. - at least one external hollow body defining at least one internal chamber; - at least one fixed disrupting grid housed inside said internal chamber to define at least one loading chamber of biologic substances to be disrupted and at least one collecting chamber of disrupted biologic substances, said fixed disrupting grid being composed of a case with passages, of a substantially axial-symmetrical shape with respect to at least one rotor, to delimit said loading chamber of biologic substances to be disrupted around a rotation axis of said rotor and said collecting chamber of disrupted biologic substances between said fixed disrupting grid and said external hollow body; and - said at least one rotor rotating inside said internal chamber to take said biologic substances in contact with said fixed disrupting grid and to cooperate with said fixed disrupting grid to allow a disruption and a passage from said loading chamber to said collecting chamber of said disrupted biologic substances through said fixed disrupting grid; said rotor comprises at least one blade, radially projecting with respect to said rotation axis of said rotor, said blade extending substantially for the whole height of said fixed disrupting grid, said blade being designed to take said biologic substances in contact with said fixed disrupting grid, characterized in that said blade comprising at least one convex surface with tapered end, in contact with said biologic substances to be taken in contact with said fixed disrupting grid; and in that said fixed disrupting grid comprises a distribution of holed micro-cells, said holed micro-cells being equipped with cutting edges around said rotation axis of said rotor. said rotor comprises at least one blade, radially projecting with respect to said rotation axis of said rotor, said blade extending substantially for the whole height of said fixed disrupting grid, said blade being designed to take said biologic substances in contact with said fixed disrupting grid, characterized in that said blade comprising at least one convex surface with tapered end, in contact with said biologic substances to be taken in contact with said fixed disrupting grid; and in that said fixed disrupting grid comprises a distribution of holed micro-cells, said holed micro-cells being equipped with cutting edges around said rotation axis of said rotor.