mhurhangee/ep-ind-claims huggingface dataset:
A method of expressing a peptide in a mammalian cell comprising: providing a nucleic acid construct including at least a nucleic acid encoding at least a NELL peptide in frame with a nucleic acid encoding a non-insect signal peptide; transfecting a mammalian cell with the nucleic acid construct; culturing the mammalian cell under conditions that permit expression of the NELL peptide, collecting a crude solution comprising the NELL peptide secreted from the mammalian cell; and purifying the NELL peptide, wherein the non-insect signal peptide is a non-insect secretory signal peptide; and wherein purifying comprises subjecting the crude solution to a cation-exchange chromatography process to obtain a purified NELL peptide, wherein said cation-exchange chromatography process comprises a medium comprising a metal ion bearing two or more charges, wherein said metal ions are included in a buffer solution or elution solution. providing a nucleic acid construct including at least a nucleic acid encoding at least a NELL peptide in frame with a nucleic acid encoding a non-insect signal peptide; transfecting a mammalian cell with the nucleic acid construct; culturing the mammalian cell under conditions that permit expression of the NELL peptide, collecting a crude solution comprising the NELL peptide secreted from the mammalian cell; and purifying the NELL peptide, wherein the non-insect signal peptide is a non-insect secretory signal peptide; and wherein purifying comprises subjecting the crude solution to a cation-exchange chromatography process to obtain a purified NELL peptide, wherein said cation-exchange chromatography process comprises a medium comprising a metal ion bearing two or more charges, wherein said metal ions are included in a buffer solution or elution solution.
A method for securing access to a resource, the method comprising performing, by a computer system: segmenting a set of previous access requests into a plurality of time-based subsets of access requests, each previous access request of the set including one or more parameters that involve one or more conditions of a potential access rule; determining a plurality of predictive percentages including a predictive percentage for each of the plurality of time-based subsets based on validity information corresponding to each previous access request of the time-based subsets, the validity information indicating whether each access request of the plurality of previous access requests is valid or invalid; determining a detection stability rating of the potential access rule by comparing the plurality of predictive percentages; and selecting the potential access rule to be a candidate access rule for use in an operational set of access rules based on the detection stability rating of the potential access rule compared to detection stability ratings of other access rules, wherein the candidate access rule is included in the operational set of access rules, the operational set of access rules is loaded into a system memory, and the operational set of access rules is used to determine an access request outcome for a real-time access request for the resource. segmenting a set of previous access requests into a plurality of time-based subsets of access requests, each previous access request of the set including one or more parameters that involve one or more conditions of a potential access rule; determining a plurality of predictive percentages including a predictive percentage for each of the plurality of time-based subsets based on validity information corresponding to each previous access request of the time-based subsets, the validity information indicating whether each access request of the plurality of previous access requests is valid or invalid; determining a detection stability rating of the potential access rule by comparing the plurality of predictive percentages; and selecting the potential access rule to be a candidate access rule for use in an operational set of access rules based on the detection stability rating of the potential access rule compared to detection stability ratings of other access rules, wherein the candidate access rule is included in the operational set of access rules, the operational set of access rules is loaded into a system memory, and the operational set of access rules is used to determine an access request outcome for a real-time access request for the resource.
A radiation detector using gas amplification, comprising: a first electrode pattern which is formed on a first surface of an insulating member and has a plurality of circular openings; and a second electrode pattern which is formed on a second surface of said insulating member opposite to said first surface thereof and has columnar portions of which respective forefronts are exposed to centers of said openings of said first electrode pattern, wherein a predetermined electric potential is set between said first electrode pattern and said second electrode pattern; the radiation detector being characterized in that edges of said first electrode pattern exposed to said openings are shaped in respective continuous first curved surfaces by covering said edges thereof with a first solder material and/or said columnar portions of said second electrode pattern are shaped in respective second continuous curved surfaces by covering said columnar portions thereof with a second solder material. a first electrode pattern which is formed on a first surface of an insulating member and has a plurality of circular openings; and a second electrode pattern which is formed on a second surface of said insulating member opposite to said first surface thereof and has columnar portions of which respective forefronts are exposed to centers of said openings of said first electrode pattern, wherein a predetermined electric potential is set between said first electrode pattern and said second electrode pattern; the radiation detector being characterized in that edges of said first electrode pattern exposed to said openings are shaped in respective continuous first curved surfaces by covering said edges thereof with a first solder material and/or said columnar portions of said second electrode pattern are shaped in respective second continuous curved surfaces by covering said columnar portions thereof with a second solder material.
A reusable diaper comprising: a forward waist portion with a forward liquid-resistant region; a rearward waist portion with a rearward liquid-resistant region; a crotch portion between the forward waist portion and the rearward waist portion; and a forward and a rearward liquid-absorbent flap (156, 158), each having a fixed end portion coupled to the diaper and a free end portion opposite the fixed end portion, which is not coupled to the reusable diaper, wherein the fixed end portion of the forward flap is closer to the forward liquid-resistant region than it is to the rearward liquid resistant region and the fixed end portion of the rearward flap is closer to the rearward liquid-resistant region than it is to the forward liquid resistant region, wherein each of said forward and a rearward liquid-absorbent flap (156, 158) is pivotably movable about its fixed end portion (160, 162), such that the forward and rearward liquid-absorbent flaps are positionable in an overlapping manner along the crotch portion with one of the forward and rearward liquid-absorbent flaps overlapped by the other one of said forward and rearward liquid-absorbent flaps and slidable relative to each other when overlapped, so as to accommodate change to the crotch length. a forward waist portion with a forward liquid-resistant region; a rearward waist portion with a rearward liquid-resistant region; a crotch portion between the forward waist portion and the rearward waist portion; and a forward and a rearward liquid-absorbent flap (156, 158), each having a fixed end portion coupled to the diaper and a free end portion opposite the fixed end portion, which is not coupled to the reusable diaper, wherein the fixed end portion of the forward flap is closer to the forward liquid-resistant region than it is to the rearward liquid resistant region and the fixed end portion of the rearward flap is closer to the rearward liquid-resistant region than it is to the forward liquid resistant region, wherein each of said forward and a rearward liquid-absorbent flap (156, 158) is pivotably movable about its fixed end portion (160, 162), such that the forward and rearward liquid-absorbent flaps are positionable in an overlapping manner along the crotch portion with one of the forward and rearward liquid-absorbent flaps overlapped by the other one of said forward and rearward liquid-absorbent flaps and slidable relative to each other when overlapped, so as to accommodate change to the crotch length.
A fluid fitting assembly comprising: a fluid fitting, wherein the fluid fitting comprises a tube, wherein the fluid fitting further comprises a fluid fitting flange; a fluid fitting retainer, wherein an inner surface of fluid fitting retainer is configured to seat against a radially outer surface of the fluid fitting flange, wherein the fluid fitting retainer comprises a retainer flange; and an alignment feature for aligning said fluid fitting with said fluid fitting retainer, wherein the alignment feature is disposed on an outer diameter, or OD, of the fluid fitting, wherein the alignment feature is located adjacent to the fluid fitting flange, wherein the alignment feature comprises a first portion, wherein the first portion comprises an annular ring, wherein an OD surface of the annular ring is parallel to an OD surface of the fluid fitting, forming a cylindrical surface; and characterized by the retainer flange comprising a first flange and a second flange, wherein an inner diameter, or ID, of the first flange is greater than an ID of the second flange, wherein an ID of the second flange is greater than an OD of the first portion. a fluid fitting, wherein the fluid fitting comprises a tube, wherein the fluid fitting further comprises a fluid fitting flange; a fluid fitting retainer, wherein an inner surface of fluid fitting retainer is configured to seat against a radially outer surface of the fluid fitting flange, wherein the fluid fitting retainer comprises a retainer flange; and an alignment feature for aligning said fluid fitting with said fluid fitting retainer, wherein the alignment feature is disposed on an outer diameter, or OD, of the fluid fitting, wherein the alignment feature is located adjacent to the fluid fitting flange, wherein the alignment feature comprises a first portion, wherein the first portion comprises an annular ring, wherein an OD surface of the annular ring is parallel to an OD surface of the fluid fitting, forming a cylindrical surface; and characterized by the retainer flange comprising a first flange and a second flange, wherein an inner diameter, or ID, of the first flange is greater than an ID of the second flange, wherein an ID of the second flange is greater than an OD of the first portion.
A writable magnetic element comprising a stack of layers presenting a write magnetic layer, the stack has a central layer (13, 53, 100) of at least one magnetic material presenting magnetization having a magnetization direction that is parallel to the plane of the central layer (13, 53, 100), which layer is sandwiched between first and second outer layers (12, 52, 101; 14, 54, 102) of non-magnetic materials, the first outer layer (12, 52, 101) comprising a first non-magnetic material and the second outer layer (14, 54, 102) comprising a second non-magnetic material that is different from the first non-magnetic material, at least the second non-magnetic material being electrically conductive, and in that it includes a device to cause a write current to pass through the second outer layer (14, 54, 102) and the central layer (13, 53, 100) in a current flow direction parallel to the plane of the central layer (13, 53, 100) at an angle α lying in the range 90° ± 60°, in particular 90° ± 30°, and more particularly 90° ± 15° relative to said magnetization direction in order to generate an effective magnetic field in the central layer (13, 53, 100), the current being applied either in a first direction or in a second direction opposite to the first, in order to orient the magnetization direction in a first magnetization direction or in a second magnetization direction opposite to the first, characterized in that the magnetization direction is oriented in response to the spin-orbit field generated by the writing current.
A sprayer device comprising a tank configured to be carried by a human operator; a fluid outlet opening in the tank; a spray nozzle connected to the outlet opening by one or more conduits; a pump assembly which comprises a pump cylinder, a reciprocating piston connected to a bell crank operative to pump fluid from the outlet opening, through said at least one conduit and out of the spray nozzle; a leakage fluid collection chamber for collecting fluid that leaks from the pump and/or the tank and/or said at least one conduit: and at least one fluid return conduit connecting the leakage fluid collection chamber to the tank; wherein the pump assembly is additionally operative to pump leakage fluid that enters the leakage fluid collection chamber through said at least one return conduit and back into the tank, said pump assembly being further characterized by: a leakage barrier comprising a sealing ring with interior lips which seal against the cylinder thereby retaining, within the collection chamber, any leakage fluid that has entered the collection chamber until that leakage fluid has been emptied from the collection chamber by downstrokes of the piston causing that leakage fluid to be returned to the tank through said at least one fluid return conduit. and at least one fluid return conduit connecting the leakage fluid collection chamber to the tank; wherein the pump assembly is additionally operative to pump leakage fluid that enters the leakage fluid collection chamber through said at least one return conduit and back into the tank, said pump assembly being further characterized by: a leakage barrier comprising a sealing ring with interior lips which seal against the cylinder thereby retaining, within the collection chamber, any leakage fluid that has entered the collection chamber until that leakage fluid has been emptied from the collection chamber by downstrokes of the piston causing that leakage fluid to be returned to the tank through said at least one fluid return conduit.
Heat exchanger for a transformer having a heat exchanger medium, comprising a feed nozzle, a heat exchanger element and a discharge nozzle, wherein the heat exchanger medium can be led via the feed nozzle into the heat exchanging element and can be led via the discharge nozzle out of the heat exchanging element, characterized in that in the feed nozzle there is disposed a first opening, in which, during operation of the heat exchanger, a first temperature probe is positioned at a predetermined location in the feed current, and in that in the discharge nozzle there is disposed a second opening, in which, during operation of the heat exchanger, a second temperature probe is positioned at a predetermined location in the discharge current, so that a first temperature measurement in the feed current of the heat exchanger medium a second temperature measurement in the discharge current of the heat exchanging medium is effected.
An IL-22 dimer for use in a method of treating a disease in a human individual, wherein the method comprises intravenously administering to the human individual an effective amount of the IL-22 dimer, wherein the amount of the IL-22 dimer is 2 μg/kg to 200 μg/kg, wherein the IL-22 dimer comprises two monomeric subunits, wherein each monomeric subunit comprises an IL-22 domain and a dimerization domain, wherein the dimerization domain comprises the CH2 and CH3 domains of human IgG, and wherein the disease is selected from the group consisting of a metabolic disease, fatty liver, viral hepatitis, multiple organ dysfunction syndrome (MODS) and a neurological disorder.
An arrangement of two components (12, 13, 30) of a wind power plant, wherein a first component (12, 13) has a flange (15, 130) with a flange contact surface (121, 122, 133) and wherein the second component has a flange-mounting surface (131, 132) for the flange (15, 130) of the first component (12, 13) or the second component (12, 13) has a flange (15, 130) with a flange contact surface (122, 133), wherein the flange contact surface (122, 133) of the first component (12, 13) and the flange-mounting surface (131, 132) of the second component are arranged opposite each other or wherein the flange contact surfaces (122, 121) of both components (12, 13, 30, 130) are arranged opposite each other, characterized in that at least one flange contact surface (121, 122, 133) of a flange (15, 130) and/or the flange-mounting surface (131, 132) of the second component has an outer friction-coefficient-increasing coating made of a chromium steel alloy or both flange contact surfaces (121, 122, 133) of the flange have an outer friction-coefficient-increasing coating made of a chromium steel alloy, wherein the connection of the components (12, 13, 30, 130) is designed as a frictional connection.
A fuel cartridge assembly for a gas turbine engine combustor, comprising: - a centerbody comprising: - an aft portion comprising at least a first fuel manifold and a second fuel manifold; - a main body extending from the aft portion and having a first passageway contained therein; - a plurality of fuel passages extending axially from the aft portion to a tip plate, each fuel passage of the plurality of fuel passages in communication with the first fuel manifold or the second fuel manifold; and the tip plate coupled to an end of the main body opposite the aft portion, the tip plate having at least one ignition opening and a plurality of openings corresponding to the plurality of axially extending fuel passages; - a spark igniter coupled to the tip plate at said ignition opening or a torch igniter coupled to the tip plate at said ignition opening; the assembly being characterized by further comprising a first outer sleeve coupled to the aft portion and a second outer sleeve coupled and coaxial to the main body; wherein the first outer sleeve and second outer sleeves are movable relative to each other. - a centerbody comprising: - an aft portion comprising at least a first fuel manifold and a second fuel manifold; - a main body extending from the aft portion and having a first passageway contained therein; - a plurality of fuel passages extending axially from the aft portion to a tip plate, each fuel passage of the plurality of fuel passages in communication with the first fuel manifold or the second fuel manifold; and the tip plate coupled to an end of the main body opposite the aft portion, the tip plate having at least one ignition opening and a plurality of openings corresponding to the plurality of axially extending fuel passages; - a spark igniter coupled to the tip plate at said ignition opening or a torch igniter coupled to the tip plate at said ignition opening; the assembly being characterized by further comprising a first outer sleeve coupled to the aft portion and a second outer sleeve coupled and coaxial to the main body; wherein the first outer sleeve and second outer sleeves are movable relative to each other.
A coated paper comprising a base paper having a coating thereon; wherein said coating comprises a binder selected from the group consisting of starch, a vinyl alcohol, a latex, a carboxymethylcellulose, and combinations thereof, and stanchion-encapsulated titanium dioxide pigment particles, wherein the pigment particles are at least partially surrounded by a polymeric coating which comprises a polymer selected from the group consisting of polyacrylate, polymethacrylate, polyalkyl(meth)acrylate, polystyrene, melamine formaldehyde, urea formaldehyde, polyvinyl alcohol, polyvinyl acetate, and combinations thereof, wherein the stanchions can form bridging between the particles or protrusions, and wherein the stanchions are of the same polymeric material as the polymeric coating.
An inhaler comprising a housing to receive a strip having a plurality of blisters, each blister having a breachable lid and containing a dose of medicament for inhalation by a user, an indexing wheel mounted in the housing rotatable to drive a strip to sequentially move blisters into alignment with a blister piercing member, a control element pivotally mounted to the housing and a drive mechanism including a drive coupling member for selectively coupling the control element to the indexing wheel so that, when coupled, the indexing wheel rotates in response to rotation of the control element to index the strip, the drive coupling member being configured to couple the control element to the indexing wheel during part of the rotation of the control element by a user so that the indexing wheel rotates together with the control element, the indexing wheel being rotatably mounted to the drive coupling member, characterised in that the drive coupling member comprises an indexing wheel drive dog, the drive mechanism including means to move, as the control element and drive coupling member are rotated, the indexing wheel drive dog into a position in which it cooperates with the indexing wheel so that the indexing wheel rotates together with the control element and the drive coupling member.
Method of preparing a solution comprising organofunctional alkali siliconates, silicates and optionally co-condensation products thereof, comprising the steps of: 1) preparing a mixture comprising at least one organofunctional silane of general formula I, at least one halosilane of formula II and alcohol, by reacting - at least one organofunctional silane of general formula I (R 1) x SiHal (y-x) with R 1 independently an organofunctional radical, with hydrolysable residue Hal, which is independently chlorine or bromine, x is 1 or 2 and y is 4, and - at least one halosilane of formula II Si(Hal) 4 with hydrolysable residue Hal, which is independently chlorine or bromine, and at least one alcohol and optionally water, 2) introducing the reacted mixture from step 1 in the presence of inert gas into an aqueous alkali hydroxide solution, wherein the inert gas is supplied in the process so that it introduces the mixture from step 1 a little at a time directly into the aqueous alkaline solution. 1) preparing a mixture comprising at least one organofunctional silane of general formula I, at least one halosilane of formula II and alcohol, by reacting - at least one organofunctional silane of general formula I (R 1) x SiHal (y-x) with R 1 independently an organofunctional radical, with hydrolysable residue Hal, which is independently chlorine or bromine, x is 1 or 2 and y is 4, and - at least one halosilane of formula II Si(Hal) 4 with hydrolysable residue Hal, which is independently chlorine or bromine, and at least one alcohol and optionally water, - at least one organofunctional silane of general formula I (R 1) x SiHal (y-x) with R 1 independently an organofunctional radical, with hydrolysable residue Hal, which is independently chlorine or bromine, x is 1 or 2 and y is 4, and - at least one halosilane of formula II Si(Hal) 4 with hydrolysable residue Hal, which is independently chlorine or bromine, and at least one alcohol and optionally water, 2) introducing the reacted mixture from step 1 in the presence of inert gas into an aqueous alkali hydroxide solution, wherein the inert gas is supplied in the process so that it introduces the mixture from step 1 a little at a time directly into the aqueous alkaline solution.