A culture device, comprising: a body comprising a self-supporting substrate having a first major surface and a second major surface; a first adhesive composition disposed on a portion of the first major surface of the substrate; a substantially dry, cold-water-soluble first hydrogel-forming composition adhered to the first adhesive composition; and a plurality of indicator agents, the plurality of indicator agents comprising: three indicator agents for detecting distinct glycosidase enzyme activities, the three indicator agents for detecting distinct glycosidase enzyme activities comprising 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside, 5-bromo-4-chloro-3-indolyl-alpha-D-glucopyranoside, and 5-bromo-4-chloro-3-indolyl-beta-D-glucopyranoside; an indicator agent for detecting an alkyl esterase enzyme activity, wherein the indicator agent for detecting an alkyl esterase enzyme activity comprises 3-indolyl-acetate; an indicator agent for detecting a phosphatase enzyme activity, wherein the indicator agent for detecting a phosphatase enzyme activity comprises 5-bromo-4-chloro-3-indolyl-phosphate; wherein at least one of the plurality of indicator agents is disposed in the first adhesive composition or the first hydrogel-forming composition; wherein each of the plurality of indicator agents comprises a detectable reporter group. a body comprising a self-supporting substrate having a first major surface and a second major surface; a first adhesive composition disposed on a portion of the first major surface of the substrate; a substantially dry, cold-water-soluble first hydrogel-forming composition adhered to the first adhesive composition; and a plurality of indicator agents, the plurality of indicator agents comprising: three indicator agents for detecting distinct glycosidase enzyme activities, the three indicator agents for detecting distinct glycosidase enzyme activities comprising 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside, 5-bromo-4-chloro-3-indolyl-alpha-D-glucopyranoside, and 5-bromo-4-chloro-3-indolyl-beta-D-glucopyranoside; an indicator agent for detecting an alkyl esterase enzyme activity, wherein the indicator agent for detecting an alkyl esterase enzyme activity comprises 3-indolyl-acetate; an indicator agent for detecting a phosphatase enzyme activity, wherein the indicator agent for detecting a phosphatase enzyme activity comprises 5-bromo-4-chloro-3-indolyl-phosphate; wherein at least one of the plurality of indicator agents is disposed in the first adhesive composition or the first hydrogel-forming composition; wherein each of the plurality of indicator agents comprises a detectable reporter group. three indicator agents for detecting distinct glycosidase enzyme activities, the three indicator agents for detecting distinct glycosidase enzyme activities comprising 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside, 5-bromo-4-chloro-3-indolyl-alpha-D-glucopyranoside, and 5-bromo-4-chloro-3-indolyl-beta-D-glucopyranoside; an indicator agent for detecting an alkyl esterase enzyme activity, wherein the indicator agent for detecting an alkyl esterase enzyme activity comprises 3-indolyl-acetate; an indicator agent for detecting a phosphatase enzyme activity, wherein the indicator agent for detecting a phosphatase enzyme activity comprises 5-bromo-4-chloro-3-indolyl-phosphate; wherein at least one of the plurality of indicator agents is disposed in the first adhesive composition or the first hydrogel-forming composition; wherein each of the plurality of indicator agents comprises a detectable reporter group.