A method of increasing flavivirus yield per culture in VERO cells growing on a conditioned microcarrier in a bioreactor, the method comprising: transferring a plurality of seed cells into the bioreactor containing a microcarrier and an original volume of a culture medium, agitating the mixture in the bioreactor at a sufficient rate of agitation and for a sufficient time to allow the seed cells to bind to the microcarrier; cultivating the seed cells to bind to the microcarier and form a bound cell population that is from about 35 percent to about 95 percent confluent on the microcarrier; removing from the bioreactor from about 30 percent to about 88 percent of the original volume of the culture medium in the bioreactor, while leaving the microcarrier with the bound cell population in the bioreactor, to form a first reduced volume of culture medium; infecting the bound cell population with a flavivirus virus; adding fresh culture medium to the bioreactor to maintain the bound cell population; culturing the bound cell population for a sufficient time period to allow the infected, bound cell population to release a shed virus concentration into the culture medium in the bioreactor; and semi-harvesting virus by removing from the bioreactor a portion of the culture medium with a first-shed virus concentration, the portion of the culture medium removed equal to from about 65 percent to about 75 percent of the original volume of the culture medium, while leaving the microcarrier with the infected, bound cell population, and a second reduced volume of the culture medium in the bioreactor; re-feeding the bound cell population by adding to the bioreactor a second amount of a fresh culture medium sufficient to increase the second reduced volume of the culture medium in the bioreactor to approximately the original volume of the culture medium; culturing the bound cell population by agitation in the bioreactor under sufficient conditions and for a sufficient time period to allow the virus to continue to allow the infected, bound cell population to release a second-shed virus into the culture medium in the bioreactor; and harvesting virus by removing from the bioreactor at least a portion of the culture medium with the second-shed virus while leaving the microcarrier with the infected, bound cell population in the bioreactor. transferring a plurality of seed cells into the bioreactor containing a microcarrier and an original volume of a culture medium, agitating the mixture in the bioreactor at a sufficient rate of agitation and for a sufficient time to allow the seed cells to bind to the microcarrier; cultivating the seed cells to bind to the microcarier and form a bound cell population that is from about 35 percent to about 95 percent confluent on the microcarrier; removing from the bioreactor from about 30 percent to about 88 percent of the original volume of the culture medium in the bioreactor, while leaving the microcarrier with the bound cell population in the bioreactor, to form a first reduced volume of culture medium; infecting the bound cell population with a flavivirus virus; adding fresh culture medium to the bioreactor to maintain the bound cell population; culturing the bound cell population for a sufficient time period to allow the infected, bound cell population to release a shed virus concentration into the culture medium in the bioreactor; and semi-harvesting virus by removing from the bioreactor a portion of the culture medium with a first-shed virus concentration, the portion of the culture medium removed equal to from about 65 percent to about 75 percent of the original volume of the culture medium, while leaving the microcarrier with the infected, bound cell population, and a second reduced volume of the culture medium in the bioreactor; re-feeding the bound cell population by adding to the bioreactor a second amount of a fresh culture medium sufficient to increase the second reduced volume of the culture medium in the bioreactor to approximately the original volume of the culture medium; culturing the bound cell population by agitation in the bioreactor under sufficient conditions and for a sufficient time period to allow the virus to continue to allow the infected, bound cell population to release a second-shed virus into the culture medium in the bioreactor; and harvesting virus by removing from the bioreactor at least a portion of the culture medium with the second-shed virus while leaving the microcarrier with the infected, bound cell population in the bioreactor.