A method of analysis of lipoprotein particles comprising the steps of: separating a plurality of classes of lipoproteins contained in a subject sample by liquid chromatography and then detecting signals of very low density lipoproteins (VLDL), low density lipoproteins and high density lipoproteins derived from components included in the separated lipoprotein particles; and assuming that each of the lipoprotein particles is constituted of subclasses estimated from component peaks consisting of anchor peaks which are peaks whose position is determined experimentally and extra essential peaks which are peaks whose position and width are mathematically determined and then calculating an approximated curve corresponding to summation of the component peaks using said detected signals, wherein medium VLDL-anchor peak, medium LDL-anchor peak and medium HDL-anchor peak are defined based on the average VLDL size, the average LDL size, and the average HDL size in a plurality of normolipidemic middle-aged men, respectively; large VLDL-anchor peak, small LDL-anchor peak and small HDL-anchor peak are defined based on the average VLDL size, the average LDL size, and the average HDL size in the cases where the lipoprotein lipase activity is absent or very low; very large HDL-anchor peak is defined based on the average HDL size in the cases suffering from cholesterol ester transfer protein deficiency; and small VLDL-anchor peak is defined based on the average VLDL size in the type III hyperlipidemia with apo E2/2 and, wherein the elution positions and peak widths of said extra essential peaks are preset so as to be located between said anchor peaks. separating a plurality of classes of lipoproteins contained in a subject sample by liquid chromatography and then detecting signals of very low density lipoproteins (VLDL), low density lipoproteins and high density lipoproteins derived from components included in the separated lipoprotein particles; and assuming that each of the lipoprotein particles is constituted of subclasses estimated from component peaks consisting of anchor peaks which are peaks whose position is determined experimentally and extra essential peaks which are peaks whose position and width are mathematically determined and then calculating an approximated curve corresponding to summation of the component peaks using said detected signals, wherein medium VLDL-anchor peak, medium LDL-anchor peak and medium HDL-anchor peak are defined based on the average VLDL size, the average LDL size, and the average HDL size in a plurality of normolipidemic middle-aged men, respectively; large VLDL-anchor peak, small LDL-anchor peak and small HDL-anchor peak are defined based on the average VLDL size, the average LDL size, and the average HDL size in the cases where the lipoprotein lipase activity is absent or very low; very large HDL-anchor peak is defined based on the average HDL size in the cases suffering from cholesterol ester transfer protein deficiency; and small VLDL-anchor peak is defined based on the average VLDL size in the type III hyperlipidemia with apo E2/2 and, wherein the elution positions and peak widths of said extra essential peaks are preset so as to be located between said anchor peaks.