A electromagnetic sensing device for detecting magnetic nanoparticles, MNP, the electromagnetic sensing device comprising: - a reference microfluidic reservoir configured to receive a reference substance with MNP; - an analyte micro fluidic reservoir configured to receive an analyte and MNP; - an input channel for introducing the analyte into the analyte micro fluidic reservoir; - at least one first excitation magnetic coil configured to subject the reference microfluidic reservoir to an alternating magnetic field at a first frequency - at least one second excitation magnetic coil configured to subject the analyte microfluidic reservoir to an alternating magnetic field at the first frequency; - at least one third excitation magnetic coil configured to subject the reference microfluidic reservoir to an alternating magnetic field at a second frequency distinct from the first frequency; - at least one fourth excitation magnetic coil configured to subject the analyte microfluidic reservoir to an alternating magnetic field at the second frequency; - at least one first detection magnetic coil configured to detect a response magnetic field of the MNP in the reference microfluidic reservoir; - at least one second detection magnetic coil configured to detect a response magnetic field of the MNP in the analyte microfluidic reservoir; wherein the electromagnetic sensing device is structured according to a plurality of superimposed layers (L1, L2, L3, L4, L5), comprising at least three coil layers (L2, L3, L4, L5) and a reservoir layer; wherein each of the reference microfluidic reservoir is integrated into a first plate in the reservoir layer and the analyte microfluidic reservoir is integrated into a second plate in the reservoir layer; wherein the first and second detection magnetic coils (D1, D2) are integrated into a first printed-circuit-board, PCB, substrate in a first coil layer; wherein the first and second excitation magnetic coils (HF1, HF2) are integrated into the PCB substrate in a second coil layer; wherein the third and fourth excitation magnetic coils (BF1, BF2) are integrated into the PCB substrate in a third coil layer; wherein the reservoir layer is immediately between the first coil layer and one of the second and third coil layers (L3, L4). - a reference microfluidic reservoir configured to receive a reference substance with MNP; - an analyte micro fluidic reservoir configured to receive an analyte and MNP; - an input channel for introducing the analyte into the analyte micro fluidic reservoir; - at least one first excitation magnetic coil configured to subject the reference microfluidic reservoir to an alternating magnetic field at a first frequency - at least one second excitation magnetic coil configured to subject the analyte microfluidic reservoir to an alternating magnetic field at the first frequency; - at least one third excitation magnetic coil configured to subject the reference microfluidic reservoir to an alternating magnetic field at a second frequency distinct from the first frequency; - at least one fourth excitation magnetic coil configured to subject the analyte microfluidic reservoir to an alternating magnetic field at the second frequency; - at least one first detection magnetic coil configured to detect a response magnetic field of the MNP in the reference microfluidic reservoir; - at least one second detection magnetic coil configured to detect a response magnetic field of the MNP in the analyte microfluidic reservoir; wherein the electromagnetic sensing device is structured according to a plurality of superimposed layers (L1, L2, L3, L4, L5), comprising at least three coil layers (L2, L3, L4, L5) and a reservoir layer; wherein each of the reference microfluidic reservoir is integrated into a first plate in the reservoir layer and the analyte microfluidic reservoir is integrated into a second plate in the reservoir layer; wherein the first and second detection magnetic coils (D1, D2) are integrated into a first printed-circuit-board, PCB, substrate in a first coil layer; wherein the first and second excitation magnetic coils (HF1, HF2) are integrated into the PCB substrate in a second coil layer; wherein the third and fourth excitation magnetic coils (BF1, BF2) are integrated into the PCB substrate in a third coil layer; wherein the reservoir layer is immediately between the first coil layer and one of the second and third coil layers (L3, L4).