An anion exchange stationary phase for separating a plurality of carbohydrates comprises: a) a negatively charged substrate particle; b) a base condensation polymer layer attached to the negatively charged substrate particle, the base condensation polymer layer comprises a first plurality of quaternary ammonium groups which the first plurality of quaternary ammonium groups are spaced apart by a first spacer, in which the base condensation polymer layer does not have a hydroxy group spaced apart from one of the first plurality of quaternary ammonium groups by an ethyl group; c) a polyalkylpolyamine condensation polymer layer formed from a polymerization reaction with a polyalkylpolyamine and a polyhalohydrocarbon, wherein all of the amine groups of the polyalkylpolyamine are tertiary amines, in which the polyalkylpolyamine condensation polymer layer is covalently attached to the base condensation polymer layer and comprises a polymeric branch structure, the polymeric branch structure includes a second plurality of quaternary ammonium groups in which the second plurality of quaternary ammonium groups are spaced apart by the first spacer, wherein the polymeric branch structure comprises a hyperbranched polymer which has a structure that has an increasing number of branches as the polymer layer extends outwardly from the negatively charged substrate particle thereby having a density of the second plurality of quaternary ammonium groups which increases in a direction away from the base condensation polymer layer, in which the polyalkylpolyamine condensation polymer layer does not have a hydroxy group spaced apart from one of the second plurality of quaternary ammonium groups by an ethyl group; wherein the first spacer comprises a chemical formula of (-CH 2 -) x where x is from 3 to 10, preferably from 3 to 6. a) a negatively charged substrate particle; b) a base condensation polymer layer attached to the negatively charged substrate particle, the base condensation polymer layer comprises a first plurality of quaternary ammonium groups which the first plurality of quaternary ammonium groups are spaced apart by a first spacer, in which the base condensation polymer layer does not have a hydroxy group spaced apart from one of the first plurality of quaternary ammonium groups by an ethyl group; c) a polyalkylpolyamine condensation polymer layer formed from a polymerization reaction with a polyalkylpolyamine and a polyhalohydrocarbon, wherein all of the amine groups of the polyalkylpolyamine are tertiary amines, in which the polyalkylpolyamine condensation polymer layer is covalently attached to the base condensation polymer layer and comprises a polymeric branch structure, the polymeric branch structure includes a second plurality of quaternary ammonium groups in which the second plurality of quaternary ammonium groups are spaced apart by the first spacer, wherein the polymeric branch structure comprises a hyperbranched polymer which has a structure that has an increasing number of branches as the polymer layer extends outwardly from the negatively charged substrate particle thereby having a density of the second plurality of quaternary ammonium groups which increases in a direction away from the base condensation polymer layer, in which the polyalkylpolyamine condensation polymer layer does not have a hydroxy group spaced apart from one of the second plurality of quaternary ammonium groups by an ethyl group;