A method of performing electrophoresis, the method comprising the steps of a) providing an electrophoretic system comprising a capillary electrophoresis gel, the gel having a loading end and an elution end; b) selecting more than one sample, each sample comprising polynucleotides of a different size and having an electrophoretic mobility that corresponds to that polynucleotides size wherein the smallest polynucleotide in a sample has the fastest electrophoretic mobility and the largest polynucleotide in a sample has the slowest electrophoretic mobility; c) loading a first sample onto the loading end of the gel at time T 0 = 0, wherein upon performing electrophoresis the polynucleotide having the fastest electrophoretic mobility in the first sample migrates past a detection window at time T F and the polynucleotide having the slowest electrophoretic mobility in the first sample migrates past the detection window at time T S; d) performing electrophoresis on the first sample; e) loading a second sample onto the loading end of the gel at time (T S -T F) and performing electrophoresis on the second sample such that the polynucleotide with the slowest electrophoretic mobility in the first sample migrates past the detection window in the electrophoretic system before the polynucleotide with the fastest electrophoretic mobility in the second sample migrates past the detection window; f) loading a third sample onto the loading end of the gel at time 2 × (T S -T F) and performing electrophoresis on the third sample such that the polynucleotide with the slowest electrophoretic mobility in the second sample migrates past the detection window in the electrophoretic system before the polynucleotide with the fastest electrophoretic mobility in the third sample migrates past the detection window; g) sequentially loading additional samples, wherein each additional sample, numbered sequentially as (n + 1), is being loaded onto the loading end of the gel at time n × (T S -T F), where n is an integer between 3 and 24, and wherein electrophoresis is performed on each sample such that the polynucleotide with the slowest electrophoretic mobility from each preceding sample migrates past the detection window before the polynucleotide with the fastest electrophoretic mobility in each succeeding sample migrates past the detection window; h) detecting polynucleotides from each sample as they migrate past the detection window. a) providing an electrophoretic system comprising a capillary electrophoresis gel, the gel having a loading end and an elution end; b) selecting more than one sample, each sample comprising polynucleotides of a different size and having an electrophoretic mobility that corresponds to that polynucleotides size wherein the smallest polynucleotide in a sample has the fastest electrophoretic mobility and the largest polynucleotide in a sample has the slowest electrophoretic mobility; c) loading a first sample onto the loading end of the gel at time T 0 = 0, wherein upon performing electrophoresis the polynucleotide having the fastest electrophoretic mobility in the first sample migrates past a detection window at time T F and the polynucleotide having the slowest electrophoretic mobility in the first sample migrates past the detection window at time T S; d) performing electrophoresis on the first sample; e) loading a second sample onto the loading end of the gel at time (T S -T F) and performing electrophoresis on the second sample such that the polynucleotide with the slowest electrophoretic mobility in the first sample migrates past the detection window in the electrophoretic system before the polynucleotide with the fastest electrophoretic mobility in the second sample migrates past the detection window; f) loading a third sample onto the loading end of the gel at time 2 × (T S -T F) and performing electrophoresis on the third sample such that the polynucleotide with the slowest electrophoretic mobility in the second sample migrates past the detection window in the electrophoretic system before the polynucleotide with the fastest electrophoretic mobility in the third sample migrates past the detection window; g) sequentially loading additional samples, wherein each additional sample, numbered sequentially as (n + 1), is being loaded onto the loading end of the gel at time n × (T S -T F), where n is an integer between 3 and 24, and wherein electrophoresis is performed on each sample such that the polynucleotide with the slowest electrophoretic mobility from each preceding sample migrates past the detection window before the polynucleotide with the fastest electrophoretic mobility in each succeeding sample migrates past the detection window; h) detecting polynucleotides from each sample as they migrate past the detection window.