A surface plasmon resonance fluorescence analysis method of detecting presence or an amount of a detection object substance by detecting fluorescence that is emitted by a fluorescence material labelling the detection object substance when the fluorescence material is excited by localized light based on a surface plasmon resonance, the surface plasmon resonance fluorescence analysis method comprising: applying excitation light from an excitation light irradiating section to an analysis chip installed in a chip holder, the analysis chip including a prism including an incidence surface and a film formation surface, a metal film disposed on the film formation surface, a capturing body fixed on the metal film, and a channel closure disposed on the film formation surface or the metal film; detecting fluorescence emitted from the fluorescence material labelling the detection object substance captured by the capturing body; acquiring output information of the excitation light irradiating section by detecting excitation light reflected by the analysis chip, wherein in the acquiring the output information of the excitation light irradiating section, reflection light from the incidence surface or from the incidence surface and a surface adjacent to the incidence surface, wherein the surface adjacent to the incidence surface is the channel closure or a bottom surface of the prism, is detected; and determining whether the excitation light irradiating section is in a normal condition by comparing the output information of the excitation light irradiating section with preliminarily acquired output information that serves as a reference of the excitation light irradiating section, wherein the output information of the excitation light irradiating section is information relating to a power of the excitation light irradiating section and an irradiation direction of excitation light of the excitation light irradiating section. applying excitation light from an excitation light irradiating section to an analysis chip installed in a chip holder, the analysis chip including a prism including an incidence surface and a film formation surface, a metal film disposed on the film formation surface, a capturing body fixed on the metal film, and a channel closure disposed on the film formation surface or the metal film; detecting fluorescence emitted from the fluorescence material labelling the detection object substance captured by the capturing body; acquiring output information of the excitation light irradiating section by detecting excitation light reflected by the analysis chip, wherein in the acquiring the output information of the excitation light irradiating section, reflection light from the incidence surface or from the incidence surface and a surface adjacent to the incidence surface, wherein the surface adjacent to the incidence surface is the channel closure or a bottom surface of the prism, is detected; and determining whether the excitation light irradiating section is in a normal condition by comparing the output information of the excitation light irradiating section with preliminarily acquired output information that serves as a reference of the excitation light irradiating section, wherein the output information of the excitation light irradiating section is information relating to a power of the excitation light irradiating section and an irradiation direction of excitation light of the excitation light irradiating section.