mhurhangee/ep-ind-claims huggingface dataset:
An in vitro method for quantitative determination of the expression of Programmed Death Ligand 1 ( PD-L1) in a liquid biopsy sample of Circulating Tumor Cells, said method comprising the steps: i. subjecting the liquid biopsy sample to reverse transcription using RNA present in the sample as a template in order to synthesize a corresponding cDNA sequence, ii. forming a reaction mixture comprising the sample, nucleic acid amplification reagents, a target primer pair, a target hydrolysis probe, said target primer pair and target hydrolysis probe being capable of hybridizing to PD-L1 mRNA, and said target primer pair consisting of a forward target primer comprising the sequence 5'-GCTGAATTGGTCATCCCAGAA-3' (SEQ ID NO: 4) and a reverse target primer comprising the sequence 5'-TTTCACATCCATCATTCTCCCTT-3' (SEQ ID NO: 6) iii. subjecting the reaction mixture to amplification conditions optimized to generate at least one copy of a nucleic acid sequence complementary to a target sequence, said target sequence being a mRNA transcript of the PD-L1 mRNA sequence (SEQ ID NO: 1), and iv. determining the amount of PD-L1 mRNA in the liquid biopsy sample, v. normalizing the expression of PD-L1 with respect to an expression of a reference gene whose expression levels remain constant between different cell types, vi. comparing the amount of PD-L1 mRNA expressed in the said sample to a positive control which is a sample comprising PD-L1 mRNA and a negative control which is a sample without comprising PD-L1 mRNA in order to estimate an overexpression of the PD-L1 mRNA sequence. i. subjecting the liquid biopsy sample to reverse transcription using RNA present in the sample as a template in order to synthesize a corresponding cDNA sequence, ii. forming a reaction mixture comprising the sample, nucleic acid amplification reagents, a target primer pair, a target hydrolysis probe, said target primer pair and target hydrolysis probe being capable of hybridizing to PD-L1 mRNA, and said target primer pair consisting of a forward target primer comprising the sequence 5'-GCTGAATTGGTCATCCCAGAA-3' (SEQ ID NO: 4) and a reverse target primer comprising the sequence 5'-TTTCACATCCATCATTCTCCCTT-3' (SEQ ID NO: 6) iii. subjecting the reaction mixture to amplification conditions optimized to generate at least one copy of a nucleic acid sequence complementary to a target sequence, said target sequence being a mRNA transcript of the PD-L1 mRNA sequence (SEQ ID NO: 1), and iv. determining the amount of PD-L1 mRNA in the liquid biopsy sample, v. normalizing the expression of PD-L1 with respect to an expression of a reference gene whose expression levels remain constant between different cell types, vi. comparing the amount of PD-L1 mRNA expressed in the said sample to a positive control which is a sample comprising PD-L1 mRNA and a negative control which is a sample without comprising PD-L1 mRNA in order to estimate an overexpression of the PD-L1 mRNA sequence.
Device for securing a step iron in a concrete wall, comprising two anchor sleeves which are provided in the concrete wall and receive two parallel anchor bolts of the U-shaped step iron in a form-fitting manner, and comprising a latching device which is provided between the coupling parts formed on the one hand by the anchor sleeves and on the other hand by the anchor bolts and consists of at least one latching tongue, which is resilient in the radial direction, on one of these coupling parts (3, 6) and of a latching recess, which receives the latching tongue, on the other coupling part (6, 3), and a run-on surface for the latching tongue adjoins the latching recess at least in a circumferential direction, said run-on surface being inclined in the circumferential direction and extending in the radial direction over the engagement depth of the latching tongue, characterised in that the at least one resilient latching tongue is provided on an open spring ring which is inserted for conjoint rotation into a circumferential groove of the associated coupling part.
A lid cover for a medicine container for preventing a medicine, which is air-tightly stored in the medicine container having a lid portion that can be pierced with a needle, from leaking to an outside space when the medicine is suctioned using a syringe having the needle, the lid cover comprising: a peripheral wall portion that can be mounted to the lid portion so as to surround a piercing face of the lid portion that can be pierced with the needle; and a ceiling face portion that is continuous with an upper portion of the peripheral wall portion, and can be pierced with the needle, wherein the peripheral wall portion and the ceiling face portion are made of an elastic material, and are configured to, in a state where the peripheral wall portion is mounted to the lid portion, air-tightly store the piercing face such that the piercing face is not exposed to the outside space, while also forming a closed space between the piercing face and the ceiling face portion, the ceiling face portion has a central portion that opposes the piercing face, and can be pierced with the needle, and an outer periphery portion that is formed in a periphery of the central portion, and characterized in that the outer periphery portion is thinner than the central portion, and the entirety of the lid cover is integrally molded. a peripheral wall portion that can be mounted to the lid portion so as to surround a piercing face of the lid portion that can be pierced with the needle; and a ceiling face portion that is continuous with an upper portion of the peripheral wall portion, and can be pierced with the needle, wherein the peripheral wall portion and the ceiling face portion are made of an elastic material, and are configured to, in a state where the peripheral wall portion is mounted to the lid portion, air-tightly store the piercing face such that the piercing face is not exposed to the outside space, while also forming a closed space between the piercing face and the ceiling face portion, the ceiling face portion has a central portion that opposes the piercing face, and can be pierced with the needle, and an outer periphery portion that is formed in a periphery of the central portion, and characterized in that the outer periphery portion is thinner than the central portion, and the entirety of the lid cover is integrally molded.
A safety railing (10, 110) for formwork for floor slabs, comprising: - at least two supporting posts (11, 12, 111, 112) for one or more anti-fall protection elements (13, 113, 114, 115), - for each post (11, 12, 111, 112), a post supporting bracket (16, 17, 116, 117), provided with clamp means (18, 118) for reversible engagement with the side (19, 119) of a load-bearing beam or of a formwork panel, - said clamp means (18, 118) having a closure system of the wedge type (22, 122), - each post comprising a tubular body that has, on a first side, a lower brace for resting an anti-fall grid and, on a second side, L-shaped braces (47, 48, 49), which are fixed so that they are spaced in a vertical direction, each cooperating with a symmetrical L-shaped brace of the other of said at least two supporting posts to support an anti-fall board (113, 114, 115), characterized in that at the upper end of each post there being an engagement protrusion in the form of a cylindrical pin extending vertically only on the central axis of each post, for the engagement of a corresponding upper portion of the anti-fall grid. - at least two supporting posts (11, 12, 111, 112) for one or more anti-fall protection elements (13, 113, 114, 115), - for each post (11, 12, 111, 112), a post supporting bracket (16, 17, 116, 117), provided with clamp means (18, 118) for reversible engagement with the side (19, 119) of a load-bearing beam or of a formwork panel, - said clamp means (18, 118) having a closure system of the wedge type (22, 122), - each post comprising a tubular body that has, on a first side, a lower brace for resting an anti-fall grid and, on a second side, L-shaped braces (47, 48, 49), which are fixed so that they are spaced in a vertical direction, each cooperating with a symmetrical L-shaped brace of the other of said at least two supporting posts to support an anti-fall board (113, 114, 115),
A thermoplastic resin composition for shock absorbing members comprising 50 to 80 parts by weight of a thermoplastic resin and 20 to 50 parts by weight of a rubbery polymer having a reactive functional group which together account for 100 parts by weight, and 1 to 200 parts by weight of an inorganic filler relative to the 100 parts by weight which is the total of the thermoplastic resin and the rubbery polymer having a reactive functional group; having morphological features observed by electron microscopy such that: the thermoplastic resin and the rubbery polymer having a reactive functional group, form a continuous phase and dispersed phase, respectively, while the inorganic filler are dispersed in the continuous phase and/or the dispersed phase; and the dispersed phase of the rubbery polymer having a reactive functional group contains fine particles with a particle diameter of 1 to 100 nm of a compound resulting from a reaction between the thermoplastic resin and the rubbery polymer having a reactive functional group; and the area occupied by the fine particles account for 10% or more of the dispersed phase; and giving a load-displacement curve meeting all of the requirements, and listed below when a square prism specimen with a cross section of 12.7 mm × 12.7 mm and a height of 25.4 mm prepared by injection-molding the thermoplastic resin composition in a parallel direction to its height is subjected to a compression test in which a weight with a mass of 26 kg is allowed to fall freely onto the square prism specimen from a height of 0.5 m: the displacement when load becomes zero is 6 mm or more, the initial load is 12 kN or more and 30 kN or less, and the displacement range included in initial load ±2 kN is 4 mm or more. the thermoplastic resin and the rubbery polymer having a reactive functional group, form a continuous phase and dispersed phase, respectively, while the inorganic filler are dispersed in the continuous phase and/or the dispersed phase; and the dispersed phase of the rubbery polymer having a reactive functional group contains fine particles with a particle diameter of 1 to 100 nm of a compound resulting from a reaction between the thermoplastic resin and the rubbery polymer having a reactive functional group; and the area occupied by the fine particles account for 10% or more of the dispersed phase; and giving a load-displacement curve meeting all of the requirements, and listed below when a square prism specimen with a cross section of 12.7 mm × 12.7 mm and a height of 25.4 mm prepared by injection-molding the thermoplastic resin composition in a parallel direction to its height is subjected to a compression test in which a weight with a mass of 26 kg is allowed to fall freely onto the square prism specimen from a height of 0.5 m: the displacement when load becomes zero is 6 mm or more, the initial load is 12 kN or more and 30 kN or less, and the displacement range included in initial load ±2 kN is 4 mm or more. the displacement when load becomes zero is 6 mm or more, the initial load is 12 kN or more and 30 kN or less, and the displacement range included in initial load ±2 kN is 4 mm or more.
Method for compensating cogging torques in electric working machines, in particular in permanent magnet electric working machines, in which a compensation current (I comp) for compensating the cogging torque (M cog) is superposed on a setpoint current (I setp) for generating a desired setpoint torque (M setp), characterized in that, in the course of open-loop current control instead of closed-loop current control, a corresponding compensation voltage (U comp) is assigned to the compensation current (I comp), which compensation voltage is superposed on a basic voltage (U regulator) to be supplied to the working machine, which is determined in a current regulator at least from the setpoint current (I setp) and the measured motor current, wherein the profile, known from the rotor angle, of the cogging torque at a known rotational speed of the working machine is calculated in advance and the compensation current (I comp) is provided without a delay.
A luminescent device, wherein the luminescent device comprises a luminescent substrate and a metal layer which is disposed on the surface of the luminescent substrate and has a metal microstructure which is composed of irregularly arranged metal nano-particles; and the material for the luminescent substrate is transparent ceramic having a formula of Y 2 O 3:Eu.
A whipping agent which is free from palm based bulk edible oil and fat and free from palm based emulsifiers for use in the preparation of edible toppings comprising on a dry weight basis: - 25 to 75% by weight of a non-palm based edible oil or fat comprising at least 70% by weight of non-hardened coconut oil; - 5 to 30 % by weight of a non-palm based alpha tending emulsifier; - 1 to 15% by weight of a proteinaceous emulsifier; and - 25 to 60% by weight carbohydrates; wherein the non-palm based alpha tending emulsifier is a lactic acid ester of mono- and/or diglycerides of a non-palm based vegetable oil, wherein the fatty acids of said glycerides are saturated or unsaturated and wherein the fatty acids have 6 to 30 carbon atoms; and which non-palm based alpha tending emulsifier has been prepared from hydrogenated non-palm vegetable oils and comprises more than 75% by weight of the fatty acid residues of stearic acid (C18:0). - 25 to 75% by weight of a non-palm based edible oil or fat comprising at least 70% by weight of non-hardened coconut oil; - 5 to 30 % by weight of a non-palm based alpha tending emulsifier; - 1 to 15% by weight of a proteinaceous emulsifier; and - 25 to 60% by weight carbohydrates;
A method for evaluating the current coupling factor (k) between an electromagnetic transponder and a terminal, wherein a ratio (r) between data, representative of a voltage across an oscillating circuit (L2, C2) of the transponder and obtained for two values (R20, R21) of the resistive load, is compared with one or several thresholds.
Method for operating, in particular shifting, of, in particular for motor vehicles, comprising at least two frictionally engaged switching mechanisms (10, 20, 30, 40) each having an input side (11, 21, 31, 41) and an output side (13, 23, 33, 43), wherein - a slip value is definable based on a difference of a rotational speed of the input side (11, 21, 31, 41) and a rotational speed of the output side (13, 23, 33, 43) to one another, and wherein - each of these switching mechanisms (10, 20, 30, 40) comprises at least one power shift state, in which the input side (11, 21, 31, 41) and the output side (13, 23, 33, 43) are engaged with each other for torque transmission, and an idle switching state, in which the input side (11, 21, 31, 41) and the output side (13, 23, 33, 43) are essentially not engaged with each other, and wherein - a first of these switching mechanisms is shifted from its power shift state in its idle shift state or vice versa, and - a second of these switching mechanisms is shifted from its idle shift state to its power shift state or vice versa, depending on its slip value, only when this slip value comprises a opposite algebraic sign after a zero crossing, in particular in the sense of a slip reversion, than between the shifting and the zero crossing of the first switching mechanism. - a slip value is definable based on a difference of a rotational speed of the input side (11, 21, 31, 41) and a rotational speed of the output side (13, 23, 33, 43) to one another, and wherein - each of these switching mechanisms (10, 20, 30, 40) comprises at least one power shift state, in which the input side (11, 21, 31, 41) and the output side (13, 23, 33, 43) are engaged with each other for torque transmission, and an idle switching state, in which the input side (11, 21, 31, 41) and the output side (13, 23, 33, 43) are essentially not engaged with each other, and wherein - a first of these switching mechanisms is shifted from its power shift state in its idle shift state or vice versa, and - a second of these switching mechanisms is shifted from its idle shift state to its power shift state or vice versa, depending on its slip value, only when this slip value comprises a opposite algebraic sign after a zero crossing, in particular in the sense of a slip reversion, than between the shifting and the zero crossing of the first switching mechanism.
A sensor configured for implantation into a human body, comprising a ring shaped element, the ring shaped element having an inner diameter, an outer diameter and a thickness, the ring shaped element being electrically conductive, the sensor further comprising a coil, the coil having a length, a diameter and two free ends on opposite sides of the coil, characterized in that the electrically conductive ring shaped element is made of a charged silicone and at least the free ends of the coil are enclosed by the silicone of the ring shaped element, wherein an electrical resistance of the ring shaped element varies upon a deformation of the ring shaped element.
A method of detecting decreased expression of miR-18b and increased methylation of the 2.5 kilo base (kB) region upstream of miR-18b in a tissue biological sample from a subject, comprising: (i) measuring the expression level of miR-18b in the tissue biological sample and determining the methylation status of the 2.5 kB region upstream of the miR-18b gene in the sample using methylation specific primers following bisulphite treatment, wherein the methylation-specific primers consist of the sequence of SEQ ID NO: 4 and 5; (ii) comparing the expression level of miR-18b in the subject's sample with the mean expression level of miR-18b from one or more control biological samples; (iii) identifying whether the expression level for miR-18b in the sample is decreased in comparison to the mean expression level for miR-18b in one or more control samples, and determining whether the sample has increased methylation for the 2.5 kB region in comparison to the methylation status for the 2.5 kB region in the one or more control samples. (i) measuring the expression level of miR-18b in the tissue biological sample and determining the methylation status of the 2.5 kB region upstream of the miR-18b gene in the sample using methylation specific primers following bisulphite treatment, wherein the methylation-specific primers consist of the sequence of SEQ ID NO: 4 and 5; (ii) comparing the expression level of miR-18b in the subject's sample with the mean expression level of miR-18b from one or more control biological samples; (iii) identifying whether the expression level for miR-18b in the sample is decreased in comparison to the mean expression level for miR-18b in one or more control samples, and determining whether the sample has increased methylation for the 2.5 kB region in comparison to the methylation status for the 2.5 kB region in the one or more control samples.
An elbow material production device formed by bending to a predetermined angle a metal hollow thick walled member of a given length having a hollow passage of an approximately circular section along an axial direction of the member with both ends opened, and approximately circular or at least one pair of parallel surfaces in the periphery of the member and comprising two half dies (82a, 82b) constituting one side die arrangement formed so as to contact one side outer surface corresponding to approximately a half surface region in the longitudinal direction of the metal hollow thick walled member to be deformed, characterized in a toggle type link mechanism formed by a first guide member movably guiding a first axial member for a supporting point to a first direction perpendicular to the axial direction of the first axial member, a pair of link levers, each being pivotally connected to the first axial member at one end portion of the link lever and a second guide member movably guiding the other end portion of the link lever to a second direction perpendicular to the axial direction of the first axial member, one side die arrangement holding members (80a, 80b) for fixedly holding each of the half dies (82a, 82b), each holding member (80a, 80b) being fixedly connected to the lateral surface of the link lever, an other side die arrangement having a first contact surface and a second contact surface, the first contact surface contacting the other side outer surface of the metal hollow thick walled member in the state bent to a predetermined angle, and the second contact surface formed in the central portion contacting an outer surface portion of the first axial member so as to press the first axial member in the first direction, an other side die arrangement holding member for fixedly holding the other side die arrangement, a first moving means for moving the other side die arrangement holding member in the first direction, a second moving means for moving the first axial member in the first direction along the first guide member through contacting the one end portion of the link lever pivotally connected to the first axial member.
Slatted mattress base, with a frame which has two longitudinal beams and a number of spring slats arranged parallel to and at a distance from one another running at right angles to the longitudinal beams and supported by the longitudinal beams, where the frame has two parts which can pivot with reference to one another around a pivot axis positioned at right angles to its longitudinal direction, where the two parts can be pivoted between a service position in which they are more or less aligned with one another and form a base on which the mattress rests, and a transport position in which they are folded together, thereby occupying a smaller area compared to the service position, where each of the longitudinal beams is divided into two beam sections, where the two sections of the two longitudinal beams are joined by a hinge, and these two hinges form the aforementioned pivot axes, and the hinges are formed as double-jointed hinges in such a way as to create two parallel pivot axes closely adjacent to one another, characterised in that the double-jointed hinges are provided on the underside of the longitudinal beams and each of the beam sections has a recess on its underside to receive the double-jointed hinges in such a way that the undersides of the longitudinal beams can rest on a surface when aligned with one another, thereby avoiding a downward projection caused by the double-jointed hinges.