mhurhangee/ep-ind-claims huggingface dataset:
A method for inserting an exogenous sequence into an amplifiable locus of a mammalian cell comprising: (a) providing a mammalian cell having an endogenous target site proximal to an endogenous selectable gene within the amplifiable locus, wherein the endogenous target site is 0 to 100,000 base pairs downstream from the 3' regulatory region of the endogenous selectable gene or 0 to 100,000 base pairs upstream from the 5' regulatory region of the endogenous selectable gene, wherein the endogenous target site comprises: (i) a recognition sequence for a site specific endonuclease; (ii) a 5' flanking region 5' to the recognition sequence; and (iii) a 3' flanking region 3' to the recognition sequence; and (b) introducing a double-stranded break between the 5' and 3' flanking regions of the endogenous target site; (c) contacting the cell with a donor vector comprising from 5' to 3': (i) a donor 5' flanking region homologous to the 5' flanking region of the endogenous target site; (ii) an exogenous sequence; and (iii) a donor 3' flanking region homologous to the 3' flanking region of the endogenous target site; whereby the donor 5' flanking region, the exogenous sequence and the donor 3' flanking region are inserted between the 5' and 3' flanking regions of the endogenous target site by homologous recombination to provide a modified cell. (a) providing a mammalian cell having an endogenous target site proximal to an endogenous selectable gene within the amplifiable locus, wherein the endogenous target site is 0 to 100,000 base pairs downstream from the 3' regulatory region of the endogenous selectable gene or 0 to 100,000 base pairs upstream from the 5' regulatory region of the endogenous selectable gene, wherein the endogenous target site comprises: (i) a recognition sequence for a site specific endonuclease; (ii) a 5' flanking region 5' to the recognition sequence; and (iii) a 3' flanking region 3' to the recognition sequence; and (i) a recognition sequence for a site specific endonuclease; (ii) a 5' flanking region 5' to the recognition sequence; and (iii) a 3' flanking region 3' to the recognition sequence; and (b) introducing a double-stranded break between the 5' and 3' flanking regions of the endogenous target site; (c) contacting the cell with a donor vector comprising from 5' to 3': (i) a donor 5' flanking region homologous to the 5' flanking region of the endogenous target site; (ii) an exogenous sequence; and (iii) a donor 3' flanking region homologous to the 3' flanking region of the endogenous target site; whereby the donor 5' flanking region, the exogenous sequence and the donor 3' flanking region are inserted between the 5' and 3' flanking regions of the endogenous target site by homologous recombination to provide a modified cell. (i) a donor 5' flanking region homologous to the 5' flanking region of the endogenous target site; (ii) an exogenous sequence; and (iii) a donor 3' flanking region homologous to the 3' flanking region of the endogenous target site;
A method of screening an agent for preventing or treating Amyotrophic Lateral Sclerosis the method comprising: (a) contacting a population of astrocytes, the astrocytes having been ex-vivo differentiated from pluripotent stem cells, with the agent; (b) co-culturing the population of astrocytes of step (a) with a population of neurons under stress selected from hypoxicity, oxidative stress, glutamate toxicity or AMPA/kainate toxicity; and (c) quantifying an effect of said agent to enhance survival or neural function of the population of neurons. (a) contacting a population of astrocytes, the astrocytes having been ex-vivo differentiated from pluripotent stem cells, with the agent; (b) co-culturing the population of astrocytes of step (a) with a population of neurons under stress selected from hypoxicity, oxidative stress, glutamate toxicity or AMPA/kainate toxicity; and (c) quantifying an effect of said agent to enhance survival or neural function of the population of neurons.
A bushing to allow adjustability of a position of a headrest, the bushing comprising: a head portion having an opening and a passageway opening; a body portion defining an internal passageway extending along an axis, wherein the passageway opening of the head portion is aligned with the internal passageway of the body portion, the body portion includes a wall having an area of a reduced wall thickness, wherein the area has a thickness that is less than a thickness of the surrounding wall of the body portion such that the area is more flexible than the surrounding wall of body portion and the area of the reduced wall thickness has a substantially flat external surface, and a rib is located on an internal surface of the area of reduced wall thickness; and a moveable portion received in the opening of the head portion, wherein the moveable portion is moveable between a first position wherein a feature engages a notch of a post receivable in the internal passageway of the body portion and the passageway opening of the head portion to retain the headrest in a position, and a second position wherein the feature is disengaged from the notch of the post, allowing the post and the headrest to move relative to the bushing. a head portion having an opening and a passageway opening; a body portion defining an internal passageway extending along an axis, wherein the passageway opening of the head portion is aligned with the internal passageway of the body portion, the body portion includes a wall having an area of a reduced wall thickness, wherein the area has a thickness that is less than a thickness of the surrounding wall of the body portion such that the area is more flexible than the surrounding wall of body portion and the area of the reduced wall thickness has a substantially flat external surface, and a rib is located on an internal surface of the area of reduced wall thickness; and a moveable portion received in the opening of the head portion, wherein the moveable portion is moveable between a first position wherein a feature engages a notch of a post receivable in the internal passageway of the body portion and the passageway opening of the head portion to retain the headrest in a position, and a second position wherein the feature is disengaged from the notch of the post, allowing the post and the headrest to move relative to the bushing.
A double needle sewing method enabling changeable needle distance, for implementation on a double needle sewing machine having an XY-axis feeding mechanism, a rotatable needle bar mechanism, a needle plate rotating mechanism and a needle distance adjustment mechanism, and a distance between two needles of the sewing machine being adjustable and changeable within a set distance range during the course of stitching two sewing lines, comprising: a data acquiring step, in which a plurality of pieces of stitching information presented in sequence are acquired according to two sewing lines that are to be formed on a fabric workpiece; and each piece of the stitching information including a movement coordinate data, a rotational angle data and a needle distance data; a stitches moving step, in which the fabric workpiece is horizontally moved by the XY-axis feeding mechanism according to those movement coordinate data sequentially; a stitches rotating step, in which a needle bar of the sewing machine is brought by the rotatable needle bar mechanism to axially rotate according to those rotational angle data sequentially, and a needle plate and a shuttle hook are brought by the needle plate rotating mechanism to rotate horizontally and synchronous with the axial rotation of the needle bar also according to those rotational angle data sequentially; and the method being characterized in that it further comprises: a needle distance changing step, in which the two needles of the sewing machine are caused by the needle distance adjustment mechanism to displace according to those needle distance data sequentially, so as to change a length of the needle distance; whereby a plurality of needle distances of different lengths are produced while the two sewing lines are continuously stitched by the two needles. a data acquiring step, in which a plurality of pieces of stitching information presented in sequence are acquired according to two sewing lines that are to be formed on a fabric workpiece; and each piece of the stitching information including a movement coordinate data, a rotational angle data and a needle distance data; a stitches moving step, in which the fabric workpiece is horizontally moved by the XY-axis feeding mechanism according to those movement coordinate data sequentially; a stitches rotating step, in which a needle bar of the sewing machine is brought by the rotatable needle bar mechanism to axially rotate according to those rotational angle data sequentially, and a needle plate and a shuttle hook are brought by the needle plate rotating mechanism to rotate horizontally and synchronous with the axial rotation of the needle bar also according to those rotational angle data sequentially; and the method being characterized in that it further comprises: a needle distance changing step, in which the two needles of the sewing machine are caused by the needle distance adjustment mechanism to displace according to those needle distance data sequentially, so as to change a length of the needle distance; whereby a plurality of needle distances of different lengths are produced while the two sewing lines are continuously stitched by the two needles.
A spherical particle between 50 - 300 μM in diameter generated from a material consisting of decellularized omentum, wherein the particle encapsulates at least one viable, intact biological cell.
Fluid dispensing device comprising: a bottle for receiving a fluid, a tube, and a pressurizing device coupled between the bottle and the tube, characterised in that: the pressurizing device includes a container coupled between the bottle and the tube, a base disposed in the container, a housing engaged in the container and movable toward and away from the base and having a compartment, and an exit port communicating with the compartment of the housing, a plug engaged between the base and the housing for engaging with the exit port of the housing, a cap mounted on the housing and having an orifice, a casing disposed in the housing and attached to the cap, a valve piece engaged in the casing for engaging with the orifice of the cap, a core attached to the casing, and at least one coil attached to the container to move the core and the casing and the cap and the housing in the container, and the plug is forced to move away from the exit port of the housing and to control the fluid to flow out of the housing when the housing is moved toward the base, and the valve piece is forced away from the orifice of the cap when the housing is moved away from the base. a bottle for receiving a fluid, a tube, and a pressurizing device coupled between the bottle and the tube, the pressurizing device includes a container coupled between the bottle and the tube, a base disposed in the container, a housing engaged in the container and movable toward and away from the base and having a compartment, and an exit port communicating with the compartment of the housing, a plug engaged between the base and the housing for engaging with the exit port of the housing, a cap mounted on the housing and having an orifice, a casing disposed in the housing and attached to the cap, a valve piece engaged in the casing for engaging with the orifice of the cap, a core attached to the casing, and at least one coil attached to the container to move the core and the casing and the cap and the housing in the container, and the plug is forced to move away from the exit port of the housing and to control the fluid to flow out of the housing when the housing is moved toward the base, and the valve piece is forced away from the orifice of the cap when the housing is moved away from the base.
A modifiable band jewelry (100, 300, 400) comprising: - a band (101, 301a,b, 401) made of fibre threads or yarns, said band having one or more loops (102a,b, 302a,b, 402a,b); and - at least one ornament (103a,b, 201a,b,c, 303a,b, 403a,b) having an upper ornamental part (202a,b,c) with a lower surface (203a,b,c) connected to a lower male part (204a,b,c), said ornament being removably attached to the band with the male part being inserted into one of said loops; wherein at least 6 or 8 fibre threads or yarns are braided together to form the fibre band (101, 301a,b, 401) with several spacedly arranged loops (102a,b, 302a,b, 402a,b); and wherein a band loop (102a,b, 302a,b, 402a,b) is formed by two braided band parts (305a, 305b, 405a, 405b), each of said braided band parts being formed by at least 3 or 4 fibre threads or yarns; characterized in that the fibre band (101, 301a,b) comprises at least one strand with several spacedly arranged loops (102a,b, 302a,b), wherein all the threads or yarns of a strand form a single string between the loops, and wherein for each loop the threads are separated in two parts (305a, 305b) to form two braided string parts defining the loop. - a band (101, 301a,b, 401) made of fibre threads or yarns, said band having one or more loops (102a,b, 302a,b, 402a,b); and - at least one ornament (103a,b, 201a,b,c, 303a,b, 403a,b) having an upper ornamental part (202a,b,c) with a lower surface (203a,b,c) connected to a lower male part (204a,b,c), said ornament being removably attached to the band with the male part being inserted into one of said loops; wherein at least 6 or 8 fibre threads or yarns are braided together to form the fibre band (101, 301a,b, 401) with several spacedly arranged loops (102a,b, 302a,b, 402a,b); and wherein a band loop (102a,b, 302a,b, 402a,b) is formed by two braided band parts (305a, 305b, 405a, 405b), each of said braided band parts being formed by at least 3 or 4 fibre threads or yarns; characterized in that the fibre band (101, 301a,b) comprises at least one strand with several spacedly arranged loops (102a,b, 302a,b), wherein all the threads or yarns of a strand form a single string between the loops, and wherein for each loop the threads are separated in two parts (305a, 305b) to form two braided string parts defining the loop.
An air filtration device for a suction appliance, the filtration device comprising: - a housing (21, 22) comprising a bottom wall, - a filter arranged inside the housing (21, 22), the filter comprising a filter end facing the bottom wall, the filter extending about a longitudinal axis and extending axially along the longitudinal axis from the filter end, - a rotary cam movably mounted in rotation about a rotary cam rotation axis relative to the housing (21, 22), - drive means (11, 13, 15) configured to rotatably drive the rotary cam, characterized in that the air filtration device also comprises: - a movable mass mounted in translation relative to the housing (21, 22) and positioned axially between the bottom wall and the filter end, - return means configured to force the movable mass in translation in a direction towards the filter end along the axis of rotation of the rotary cam, the rotary cam being configured to force the movable mass in a direction away from the filter end along the axis of rotation of the rotary cam, or to release the movable mass, depending on the angular position of the rotary cam about the rotary cam rotation axis, the release of the movable mass relative to the rotary cam causing a percussion of the filter end by the movable mass due to the force of the return means on the movable mass. - a housing (21, 22) comprising a bottom wall, - a filter arranged inside the housing (21, 22), the filter comprising a filter end facing the bottom wall, the filter extending about a longitudinal axis and extending axially along the longitudinal axis from the filter end, - a rotary cam movably mounted in rotation about a rotary cam rotation axis relative to the housing (21, 22), - drive means (11, 13, 15) configured to rotatably drive the rotary cam, characterized in that the air filtration device also comprises: - a movable mass mounted in translation relative to the housing (21, 22) and positioned axially between the bottom wall and the filter end, - return means configured to force the movable mass in translation in a direction towards the filter end along the axis of rotation of the rotary cam, - a movable mass mounted in translation relative to the housing (21, 22) and positioned axially between the bottom wall and the filter end, - return means configured to force the movable mass in translation in a direction towards the filter end along the axis of rotation of the rotary cam, the rotary cam being configured to force the movable mass in a direction away from the filter end along the axis of rotation of the rotary cam, or to release the movable mass, depending on the angular position of the rotary cam about the rotary cam rotation axis, the release of the movable mass relative to the rotary cam causing a percussion of the filter end by the movable mass due to the force of the return means on the movable mass.
A multiphase synchronous electrical machine for converting kinetic energy into electrical energy or electrical energy into kinetic energy on board a transport vehicle; the electrical machine comprising a rotor extending along an axis, and a stator provided with a stator pack having slots and with an electrical winding, which extends in part in the slots and in part at two heads (28, 29) arranged on opposite sides of the stator pack, and comprises bars (30, 31, 32, 33) housed in the slots and connected to one another at the heads (28, 29); the electrical winding comprising connection plates, each of which comprises a main body (74; 75) having at least two seats (69; 80) for housing the bars (30, 31, 32, 33); the electrical machine being characterized in that each connection plates (46, 47, 48, 49 51, 52, 53; 50) comprises at least one arm (70; 76) that is set between the two seats (69; 80) and extends from the main body towards the axis.
A device for modifying, during decoding of a sound signal, a synthesis of a time-domain excitation decoded by a time-domain decoder, comprising: a classifier (104, 105, 106, 301) of the synthesis of the decoded time-domain excitation into one of a number of categories; a converter (107, 201) of the decoded time-domain excitation into a frequency-domain excitation; a modifier (107, 203, 204, 205, 206, 207, 208, 209, 210) of the frequency-domain excitation as a function of the category in which the synthesis of the decoded time-domain excitation is classified by the classifier (104, 105, 106, 301); a converter (107, 202) of the modified frequency-domain excitation into a modified time-domain excitation; a synthesis filter supplied with the modified time-domain excitation to produce a modified synthesis of the decoded time-domain excitation. a classifier (104, 105, 106, 301) of the synthesis of the decoded time-domain excitation into one of a number of categories; a converter (107, 201) of the decoded time-domain excitation into a frequency-domain excitation; a modifier (107, 203, 204, 205, 206, 207, 208, 209, 210) of the frequency-domain excitation as a function of the category in which the synthesis of the decoded time-domain excitation is classified by the classifier (104, 105, 106, 301); a converter (107, 202) of the modified frequency-domain excitation into a modified time-domain excitation; a synthesis filter supplied with the modified time-domain excitation to produce a modified synthesis of the decoded time-domain excitation.
A copolyester film comprising a copolyester which comprises repeating units derived from a diol, a dicarboxylic acid and a poly(alkylene oxide), wherein the copolyester film further comprises a first metal ion-containing component selected from conductive ceramic particulate materials, wherein said first metal ion-containing component is a lithium ion-containing component or a sodium ion-containing component, wherein the film may further comprise additional metal ions from one or more sources other than said conductive ceramic particulate material, and wherein, when present, the metal of said additional metal ions is the same as the metal of said first metal ion-containing component.
Non-medical use of an animal feed composition comprising a capsaicin product or an animal feed supplement comprising a capsaicin product, for increasing lactation in a non-avian monogastric animal.
Side window for a vehicle, having a first surface (Ia), a second surface (Ib) opposite thereto, and a lower edge, and having at least one retaining element attached in the region of the lower edge, wherein the retaining element has a contact section with a contact surface for securing to the side window and a securing section connected to the contact section for securing to the vehicle, and wherein the retaining element is secured to the side window on one side, by connecting the contact surface to the first surface (Ia) via an adhesive, characterized in that the adhesive has a thickness of at least 1 mm and a modulus of elasticity of at least 150 MPa, wherein the retaining element has a filling opening for injecting the adhesive into the intermediate space between the contact section and the first surface (Ia) of the side window, wherein the contact surface has at least one channel, which is directed toward the filling opening and is suitable for influencing the flow direction of the adhesive, wherein the contact surface has a plurality of channels, which are directed toward the filling opening and are distributed over the contact surface in a fan-like manner. wherein the retaining element has a contact section with a contact surface for securing to the side window and a securing section connected to the contact section for securing to the vehicle, and wherein the retaining element is secured to the side window on one side, by connecting the contact surface to the first surface (Ia) via an adhesive, characterized in that the adhesive has a thickness of at least 1 mm and a modulus of elasticity of at least 150 MPa, wherein the retaining element has a filling opening for injecting the adhesive into the intermediate space between the contact section and the first surface (Ia) of the side window, wherein the contact surface has at least one channel, which is directed toward the filling opening and is suitable for influencing the flow direction of the adhesive, wherein the contact surface has a plurality of channels, which are directed toward the filling opening and are distributed over the contact surface in a fan-like manner.