An immunoassay test device (10, 10a, 10b, 10c, 10d, 10e, 10', 110, 210) for use with an immunoassay conjugate having a marker, the test device comprising: a) a first sorbent material being a strip (30, 30', 130, 230) having a solution receiving location (24, 24', 124) arranged to receive a conjugate solution having the immunoassay conjugate comprising an antigen or an antibody conjugated to a colored marker when a liquid conjugate system is used, or a buffer solution when a dry conjugate system with the immunoassay conjugate comprising an antigen or an antibody conjugated to a colored marker provided on the first sorbent material is used, and defining a first horizontal migration flow path for the conjugate solution or the buffer solution and conjugate; and b) a test site (33, 33', 133, 233) having an immobilized ligand-binding mechanism and being in communication with said first horizontal migration flow path; characterized in that the immunoassay test device further comprises c) a second sorbent material being a strip (32, 32', 132, 232), the first sorbent material and the second sorbent material are separate pieces which overlie one another and the test line is printed on one or both of the sorbent materials at the junction, the second sorbent material having a second location (26, 26', 126) arranged to receive a sample, with the second sorbent material defining a second horizontal migration path for the sample to said test site which is distinct from the first migration path, and wherein said first and second sorbent materials being strips are arranged such that at the junction of said strips a test zone with said test site is defined in which immobilized antigens or antibodies or other ligand-binding molecules are located, wherein the term "distinct" when used in conjunction with the words "flow path" or "migration path" shall be understood to mean "not in fluid communication except via a test zone"; wherein said first and second sorbent materials being strips are arranged in a "T" configuration, where the first location for receiving the buffer or buffer-conjugate solution is located near one end of the top bar of the T, the second location for receiving the sample is located near the end of the stem of the T, and the sorbent materials overlie each other at the intersection; or wherein said first and second sorbent materials being strips are arranged in a "+" configuration, where the first location for receiving the buffer or buffer-conjugate solution is located near one end of a first bar, the second location for receiving the sample is located near the end of one end of the second bar, and the sorbent materials overlie each other at the intersection. a) a first sorbent material being a strip (30, 30', 130, 230) having a solution receiving location (24, 24', 124) arranged to receive a conjugate solution having the immunoassay conjugate comprising an antigen or an antibody conjugated to a colored marker when a liquid conjugate system is used, or a buffer solution when a dry conjugate system with the immunoassay conjugate comprising an antigen or an antibody conjugated to a colored marker provided on the first sorbent material is used, and defining a first horizontal migration flow path for the conjugate solution or the buffer solution and conjugate; and b) a test site (33, 33', 133, 233) having an immobilized ligand-binding mechanism and being in communication with said first horizontal migration flow path; characterized in that the immunoassay test device further comprises c) a second sorbent material being a strip (32, 32', 132, 232), the first sorbent material and the second sorbent material are separate pieces which overlie one another and the test line is printed on one or both of the sorbent materials at the junction, the second sorbent material having a second location (26, 26', 126) arranged to receive a sample, with the second sorbent material defining a second horizontal migration path for the sample to said test site which is distinct from the first migration path, and wherein said first and second sorbent materials being strips are arranged such that at the junction of said strips a test zone with said test site is defined in which immobilized antigens or antibodies or other ligand-binding molecules are located, wherein the term "distinct" when used in conjunction with the words "flow path" or "migration path" shall be understood to mean "not in fluid communication except via a test zone"; wherein said first and second sorbent materials being strips are arranged in a "T" configuration, where the first location for receiving the buffer or buffer-conjugate solution is located near one end of the top bar of the T, the second location for receiving the sample is located near the end of the stem of the T, and the sorbent materials overlie each other at the intersection; or wherein said first and second sorbent materials being strips are arranged in a "+" configuration, where the first location for receiving the buffer or buffer-conjugate solution is located near one end of a first bar, the second location for receiving the sample is located near the end of one end of the second bar, and the sorbent materials overlie each other at the intersection.