An in vitro method of isolating a biological entity from a sample comprising (i) providing a sample comprising the biological entity, wherein the biological entity comprises a surface antigen; (ii) providing a linking molecule comprising an antigen and a component for a non-covalent protein-ligand interaction, wherein the component is a ligand; (iii) providing at least two tagging agents each comprising at least one binding domain capable of specifically binding to the antigen on the biological entity or to the antigen comprised in the linking molecule, preferably wherein the tagging agents further comprise a component for a non-covalent protein-ligand interaction, wherein the component is a ligand; (iv) providing a carrier comprising at least two components for non-covalent protein-ligand interactions, wherein the components are ligand binding partners; (vi) providing a stationary phase comprising a component capable of forming a protein-ligand interaction, wherein the component is a ligand binding partner; and (vii) incubating the sample, linking molecule, tagging agents, carrier and stationary phase within a container and allowing complex formation between the biological entity, the tagging agents, the linking molecule, the carrier and the stationary phase, wherein the binding of the biological entity via the tagging agent to the carrier and the binding of the linking molecule via the tagging agent to the carrier and the binding of the linking molecule to the stationary phase are mediated by non-covalent protein-ligand interaction, wherein the biological entity is immobilized on the stationary phase; and (viii) purifying the biological entity by a chromatographic procedure; and/or (ix) isolating the biological entity by releasing the biological entity from the carrier and the tagging agent, respectively. (i) providing a sample comprising the biological entity, wherein the biological entity comprises a surface antigen; (ii) providing a linking molecule comprising an antigen and a component for a non-covalent protein-ligand interaction, wherein the component is a ligand; (iii) providing at least two tagging agents each comprising at least one binding domain capable of specifically binding to the antigen on the biological entity or to the antigen comprised in the linking molecule, preferably wherein the tagging agents further comprise a component for a non-covalent protein-ligand interaction, wherein the component is a ligand; (iv) providing a carrier comprising at least two components for non-covalent protein-ligand interactions, wherein the components are ligand binding partners; (vi) providing a stationary phase comprising a component capable of forming a protein-ligand interaction, wherein the component is a ligand binding partner; and (vii) incubating the sample, linking molecule, tagging agents, carrier and stationary phase within a container and allowing complex formation between the biological entity, the tagging agents, the linking molecule, the carrier and the stationary phase, wherein the binding of the biological entity via the tagging agent to the carrier and the binding of the linking molecule via the tagging agent to the carrier and the binding of the linking molecule to the stationary phase are mediated by non-covalent protein-ligand interaction, wherein the biological entity is immobilized on the stationary phase; and (viii) purifying the biological entity by a chromatographic procedure; and/or (ix) isolating the biological entity by releasing the biological entity from the carrier and the tagging agent, respectively.