A unitary particle processing cartridge (100, 200) adapted for insertion into and removal from an operating machine, the unitary particle processing cartridge comprising: a particle processing component configured for processing a sample, the particle processing component including an input for receiving a sample, and one or more outputs for outputting the processed sample, wherein the particle processing component comprises a microfluidic particle sorting component for sorting particles in the sample having a predetermined characteristic from particles in the sample that do not have the predetermined characteristic; a cartridge substrate providing one or more fluid chambers (112, 114, 124a, 124b) upstream and downstream of the particle processing component (110, 120), wherein at least one of the one or more fluid chambers (112, 114, 124a, 124b) is in fluid communication with the input of the particle processing component and at least one of the one or more fluid chambers (112, 114, 124a, 124b) is in fluid communication with the one or more outputs of the particle processing component; wherein each of the fluid chambers of the unitary particle processing cartridge include fluid contact surfaces and the fluid contact surfaces are encapsulated and sealed in the unitary particle processing cartridge from the exterior environment; a filter fluidically coupled to and disposed downstream of each of the one or more fluid chambers (124a, 124b) in fluid communication with the one or more outputs of the particle processing component to increase concentration of particles having the predetermined characteristic; and a sealable sample extraction port in fluid communication with at least one of the one or more fluid chambers of the cartridge substrate in fluid communication with the one or more outputs of the particle processing component, and configured for extracting a processed sample from the unitary particle processing cartridge. a particle processing component configured for processing a sample, the particle processing component including an input for receiving a sample, and one or more outputs for outputting the processed sample, wherein the particle processing component comprises a microfluidic particle sorting component for sorting particles in the sample having a predetermined characteristic from particles in the sample that do not have the predetermined characteristic; a cartridge substrate providing one or more fluid chambers (112, 114, 124a, 124b) upstream and downstream of the particle processing component (110, 120), wherein at least one of the one or more fluid chambers (112, 114, 124a, 124b) is in fluid communication with the input of the particle processing component and at least one of the one or more fluid chambers (112, 114, 124a, 124b) is in fluid communication with the one or more outputs of the particle processing component; wherein each of the fluid chambers of the unitary particle processing cartridge include fluid contact surfaces and the fluid contact surfaces are encapsulated and sealed in the unitary particle processing cartridge from the exterior environment; a filter fluidically coupled to and disposed downstream of each of the one or more fluid chambers (124a, 124b) in fluid communication with the one or more outputs of the particle processing component to increase concentration of particles having the predetermined characteristic; and a sealable sample extraction port in fluid communication with at least one of the one or more fluid chambers of the cartridge substrate in fluid communication with the one or more outputs of the particle processing component, and configured for extracting a processed sample from the unitary particle processing cartridge.