A method for determining a position of at least one emitter in a sample, wherein according to at least one measuring parameter, - the sample is illuminated by activation light to activate at least one emitter, such that the at least one emitter emits emission light when excited by excitation light, and in at least one localization step: - the sample is illuminated by an excitation light intensity distribution of the excitation light to excite the at least one emitter, wherein the excitation light intensity distribution comprises a local minimum flanked by maxima, - the excitation light intensity distribution is positioned at a plurality of probing positions determined based on an initial position value, - an emission signal from the at least one emitter is detected for each of the probing positions, - at least one final position value (P1, P2, P3) is determined from the emission signals and the associated probing positions, - a position of the at least one emitter is estimated based on the at least one final position value (P1, P2, P3), and - a speed parameter is adjusted by a user input wherein the at least one measuring parameter is adjusted based on the adjusted speed parameter, and wherein an average time between a start of the illumination of the sample by the activation light and the estimation of the position of the at least one emitter depends on the speed parameter, characterized in that in case the speed parameter has been adjusted to a value indicating a slow operating mode, the intensity of the activation light is adjusted, such that an average of one emitter is activated in a reference volume within the maxima of the excitation light distribution, and wherein in case the speed parameter has been adjusted to a value indicating a fast operating mode, the intensity of the activation light is adjusted, such that an average of at least two emitters are activated in the reference volume, wherein - a plurality of final position values (P1, P2, P3) is generated, wherein in case at least two emitters have been activated in the reference volume, a spatial distribution is generated from the final position values (P1, P2, P3), wherein the positions of the at least two emitters are simultaneously estimated based on the spatial distribution, and/or - the emitters are capable of entering an inactive state in which the emitters are unable to emit emission light or unable to be excited by the excitation light, wherein a time trace of the emission signals is obtained, wherein a stepwise decrease of the emission signal in said time trace indicates one of the emitters entering the inactive state and a stepwise increase of the emission signal in said time trace indicates one of the emitters leaving the inactive state, and wherein information from the time trace about the emitters entering or leaving the inactive state is used to simultaneously estimate the positions of the at least two emitters. - the sample is illuminated by activation light to activate at least one emitter, such that the at least one emitter emits emission light when excited by excitation light, and - the sample is illuminated by an excitation light intensity distribution of the excitation light to excite the at least one emitter, wherein the excitation light intensity distribution comprises a local minimum flanked by maxima, - the excitation light intensity distribution is positioned at a plurality of probing positions determined based on an initial position value, - an emission signal from the at least one emitter is detected for each of the probing positions, - at least one final position value (P1, P2, P3) is determined from the emission signals and the associated probing positions, - a position of the at least one emitter is estimated based on the at least one final position value (P1, P2, P3), and - a speed parameter is adjusted by a user input wherein the at least one measuring parameter is adjusted based on the adjusted speed parameter, and wherein an average time between a start of the illumination of the sample by the activation light and the estimation of the position of the at least one emitter depends on the speed parameter, - a plurality of final position values (P1, P2, P3) is generated, wherein in case at least two emitters have been activated in the reference volume, a spatial distribution is generated from the final position values (P1, P2, P3), wherein the positions of the at least two emitters are simultaneously estimated based on the spatial distribution, and/or - the emitters are capable of entering an inactive state in which the emitters are unable to emit emission light or unable to be excited by the excitation light, wherein a time trace of the emission signals is obtained, wherein a stepwise decrease of the emission signal in said time trace indicates one of the emitters entering the inactive state and a stepwise increase of the emission signal in said time trace indicates one of the emitters leaving the inactive state, and wherein information from the time trace about the emitters entering or leaving the inactive state is used to simultaneously estimate the positions of the at least two emitters.