Process for microbiological detection and determination of antimicrobial susceptibility in clinical, environmental or food samples characterized by comprising the following steps: i) Concentration of the microbiological component of a sample to be analyzed; ii) Incubation of the sample, obtained in step i), and addition of a volume of a concentrated liquid culture medium; wherein the ratio between the volume of the culture medium and the volume of the concentrated sample is of 1: 1 and is in the range of 200 μL to 2 mL; iii) Introduction of the concentrated and enriched sample, obtained in step ii), in a reservoir matrix comprising at least two wells and at least two controlled release reservoirs of antimicrobial agents, wherein each well is configured to receive a portion of the sample and is associated to a controlled release reservoir of antimicrobial agents, wherein each controlled release reservoir of antimicrobial agents is configured to release an antimicrobial agent over a portion of the sample; iv) First analysis of the sample enriched with the culture medium, obtained in step ii), by irradiating it with external energy and subsequent digital recording of the information from the first analysis to detect the presence of micromovements, wherein the external energy is originated from coherent light sources having wavelengths varying in the range from 193 nm to 2940 nm, and powers varying in a range from 0,5 mW to 400 mW; v) Controlled release of a first amount of at least one antimicrobial agent, from a controlled release reservoir of antimicrobial agents, over a portion of the sample obtained in step ii), which is disposed in the well associated with the respective controlled release reservoir of antimicrobial agents; vi) Second analysis of the sample obtained in step v), which is again irradiated with external energy and subsequent digital recording of information from the second analysis; vii) Processing of the information obtained in steps iv) and vi) using a suitable processing algorithm, with the antimicrobial susceptibility profile being issued to the end user as a result of each sample from step v); wherein steps v) and vi) are repeated at least once, wherein in a repetition of step v) the controlled release of a second amount of at least one antimicrobial agent from a controlled release reservoir of antimicrobial agents occurs, over the respective associated well including the sample portion and in which in a repetition of step vi) the digital recording of the corresponding analysis information is performed; wherein the coherent light optical properties and the reflection and / or refraction thereof allows the detection and characterization of the micromovements of the microorganisms; and wherein the microorganisms to be detected are selected from the group which consists of bacteria or fungi having rapid growth or fastidious growth. i) Concentration of the microbiological component of a sample to be analyzed; ii) Incubation of the sample, obtained in step i), and addition of a volume of a concentrated liquid culture medium; wherein the ratio between the volume of the culture medium and the volume of the concentrated sample is of 1: 1 and is in the range of 200 μL to 2 mL; iii) Introduction of the concentrated and enriched sample, obtained in step ii), in a reservoir matrix comprising at least two wells and at least two controlled release reservoirs of antimicrobial agents, wherein each well is configured to receive a portion of the sample and is associated to a controlled release reservoir of antimicrobial agents, wherein each controlled release reservoir of antimicrobial agents is configured to release an antimicrobial agent over a portion of the sample; iv) First analysis of the sample enriched with the culture medium, obtained in step ii), by irradiating it with external energy and subsequent digital recording of the information from the first analysis to detect the presence of micromovements, wherein the external energy is originated from coherent light sources having wavelengths varying in the range from 193 nm to 2940 nm, and powers varying in a range from 0,5 mW to 400 mW; v) Controlled release of a first amount of at least one antimicrobial agent, from a controlled release reservoir of antimicrobial agents, over a portion of the sample obtained in step ii), which is disposed in the well associated with the respective controlled release reservoir of antimicrobial agents; vi) Second analysis of the sample obtained in step v), which is again irradiated with external energy and subsequent digital recording of information from the second analysis; vii) Processing of the information obtained in steps iv) and vi) using a suitable processing algorithm, with the antimicrobial susceptibility profile being issued to the end user as a result of each sample from step v); wherein steps v) and vi) are repeated at least once, wherein in a repetition of step v) the controlled release of a second amount of at least one antimicrobial agent from a controlled release reservoir of antimicrobial agents occurs, over the respective associated well including the sample portion and in which in a repetition of step vi) the digital recording of the corresponding analysis information is performed; wherein the coherent light optical properties and the reflection and / or refraction thereof allows the detection and characterization of the micromovements of the microorganisms; and wherein the microorganisms to be detected are selected from the group which consists of bacteria or fungi having rapid growth or fastidious growth.