Method based on digital holography for determining parameters of at least one erythrocyte (11, 45, 55) in a sample, the method comprising the steps of: • creating an interference pattern by superimposing, using a beam splitter, a wave front (41, 52) of at least one reference beam and a wave front of at least one object beam which has passed through the said at least one erythrocyte (11, 45, 55); wherein said at least one object beam and said at least one reference beam are mutually coherent beams using at least one light source; • detecting the interference pattern by means of a digital image sensor; • using a processor unit, reconstructing from the detected interference pattern amplitude- and phase information of the object wave front by means of a numerical reconstruction process; • representing the amplitude and phase information as a reconstructed image in each of the amplitude and phase domain; • configuring the processing unit to process the reconstructed amplitude image to obtain the total absorption (Ω) of the at least one erythrocyte according to Ω = Sε λ 0 c h ∑ i N p l i + 1 − S ε λ d c h ∑ i N p l i, where S is hemoglobin oxygen saturation, ε λ 0 is the molar absorptivity of oxyhemoglobin at wavelength λ,, c h is the hemoglobin concentration, N p is the total number of pixels representing said erythrocyte in said reconstructed amplitude image, l i is the thickness of said erythrocyte at pixel i and ε λ d is the molar absorptivity of deoxyhemoglobin at wavelength λ, and to process the reconstructed phase image to obtain the total phase shift (Φ) of the at least one erythrocyte Φ = ∑ N p φ i = 2 π n 0 i + αc h − n m i λ ∑ N p l i = 2 π n 0 i − n m i aλ ∗ V + 2 παc h λ ∑ N p l i where ( φ i) is the relative phase shift at pixel i, n o is the refractive index of an erythrocyte in the absence of hemoglobin, n m i is the refractive index of the non-erythrocyte surrounding fluid at pixel i, α is the area imaged by a pixel i, α is the refractive index increment of hemoglobin at a specific wavelength, and V = a ∑ i N p l i is the erythrocytes volume; and • determining the mean corpuscular volume or mean corpuscular hemoglobin concentration (MCHC) or the oxygen saturation or the mean corpuscular hemoglobin based on the said total absorption (Ω) and the said total phase shift (Φ). • creating an interference pattern by superimposing, using a beam splitter, a wave front (41, 52) of at least one reference beam and a wave front of at least one object beam which has passed through the said at least one erythrocyte (11, 45, 55); wherein said at least one object beam and said at least one reference beam are mutually coherent beams using at least one light source; • detecting the interference pattern by means of a digital image sensor; • using a processor unit, reconstructing from the detected interference pattern amplitude- and phase information of the object wave front by means of a numerical reconstruction process; • representing the amplitude and phase information as a reconstructed image in each of the amplitude and phase domain; • configuring the processing unit to process the reconstructed amplitude image to obtain the total absorption (Ω) of the at least one erythrocyte according to Ω = Sε λ 0 c h ∑ i N p l i + 1 − S ε λ d c h ∑ i N p l i, where S is hemoglobin oxygen saturation, ε λ 0 is the molar absorptivity of oxyhemoglobin at wavelength λ,, c h is the hemoglobin concentration, N p is the total number of pixels representing said erythrocyte in said reconstructed amplitude image, l i is the thickness of said erythrocyte at pixel i and ε λ d is the molar absorptivity of deoxyhemoglobin at wavelength λ, and to process the reconstructed phase image to obtain the total phase shift (Φ) of the at least one erythrocyte Φ = ∑ N p φ i = 2 π n 0 i + αc h − n m i λ ∑ N p l i = 2 π n 0 i − n m i aλ ∗ V + 2 παc h λ ∑ N p l i where ( φ i) is the relative phase shift at pixel i, n o is the refractive index of an erythrocyte in the absence of hemoglobin, n m i is the refractive index of the non-erythrocyte surrounding fluid at pixel i, α is the area imaged by a pixel i, α is the refractive index increment of hemoglobin at a specific wavelength, and V = a ∑ i N p l i is the erythrocytes volume; and • determining the mean corpuscular volume or mean corpuscular hemoglobin concentration (MCHC) or the oxygen saturation or the mean corpuscular hemoglobin based on the said total absorption (Ω) and the said total phase shift (Φ).