A method for testing a luminescence imaging apparatus with a testing device placed within a field of view of an imaging head of the luminescence imaging apparatus, the testing device having an imaging surface to be imaged by the luminescence imaging apparatus with one or more sites each comprising at least one luminescence substance, wherein the method comprises, under the control of a control unit of the luminescence imaging apparatus: acquiring, with a photograph camera of the imaging head, a photograph image of the field of view, the photograph image being representative of a reflected light being reflected by objects of the field of view comprising the testing device, retrieving, by an object recognition engine of the control unit of the luminescence imaging apparatus, a descriptor of the testing device from a configuration repository, the descriptor comprising an indication of a geometry of the testing device and of a position of the sites in the testing device, finding a position of the testing device in the photograph image according to the geometry of the testing device, calculating, by a testing manager of the control unit of the luminescence imaging apparatus,, a transformation matrix defining a transformation between real-word coordinates of the testing device being indicated in the descriptor and corresponding image coordinates of the testing device being found in the photograph image, acquiring, with a luminescence camera of the imaging head, a luminescence image of the field of view representative of a luminescence light being emitted by the luminescence substance of the sites in response to an excitation light thereof provided by an excitation light source of the imaging head, determining, by the testing manager, a representation of the sites in the luminescence image according to an application of the transformation to the position of the sites in the testing device being indicated in the descriptor, and testing the luminescence imaging apparatus according to the representation of the sites in the luminescence image. acquiring, with a photograph camera of the imaging head, a photograph image of the field of view, the photograph image being representative of a reflected light being reflected by objects of the field of view comprising the testing device, retrieving, by an object recognition engine of the control unit of the luminescence imaging apparatus, a descriptor of the testing device from a configuration repository, the descriptor comprising an indication of a geometry of the testing device and of a position of the sites in the testing device, finding a position of the testing device in the photograph image according to the geometry of the testing device, calculating, by a testing manager of the control unit of the luminescence imaging apparatus,, a transformation matrix defining a transformation between real-word coordinates of the testing device being indicated in the descriptor and corresponding image coordinates of the testing device being found in the photograph image, acquiring, with a luminescence camera of the imaging head, a luminescence image of the field of view representative of a luminescence light being emitted by the luminescence substance of the sites in response to an excitation light thereof provided by an excitation light source of the imaging head, determining, by the testing manager, a representation of the sites in the luminescence image according to an application of the transformation to the position of the sites in the testing device being indicated in the descriptor, and testing the luminescence imaging apparatus according to the representation of the sites in the luminescence image.