An arrangement configured for examining a plurality of samples within a sample region in or on a sample carrier, the arrangement comprising optics configured to selectively image the sample region in a transmitted light imaging mode and a lightsheet imaging mode, the transmitted light imaging mode being configured to generate and process a stack of widefield images of the sample region, obtaining a three-dimensional representation of the sample region, and the lightsheet imaging mode being configured to obtain lightsheet images of at least one of the samples at lightsheet imaging positions by providing a lightsheet exciting fluorescence in the sample and to process said lightsheet images, and the arrangement further comprising a processing apparatus configured to perform, in the sample region, the steps of a) providing operating instructions causing the arrangement to be operated in the transmitted light imaging mode, b) selecting one or more regions of interest in the sample region based on selection information obtained on the basis of the stack of widefield images generated in the transmitted light imaging mode, c) providing position information relating to the one or more regions of interest selected in the sample region, and d) providing operating instructions causing the arrangement to be operated in the lightsheet imaging mode at the one or more lightsheet imaging positions on the basis of the position information. the arrangement comprising optics configured to selectively image the sample region in a transmitted light imaging mode and a lightsheet imaging mode, the transmitted light imaging mode being configured to generate and process a stack of widefield images of the sample region, obtaining a three-dimensional representation of the sample region, and the lightsheet imaging mode being configured to obtain lightsheet images of at least one of the samples at lightsheet imaging positions by providing a lightsheet exciting fluorescence in the sample and to process said lightsheet images, and the arrangement further comprising a processing apparatus configured to perform, in the sample region, the steps of a) providing operating instructions causing the arrangement to be operated in the transmitted light imaging mode, b) selecting one or more regions of interest in the sample region based on selection information obtained on the basis of the stack of widefield images generated in the transmitted light imaging mode, c) providing position information relating to the one or more regions of interest selected in the sample region, and d) providing operating instructions causing the arrangement to be operated in the lightsheet imaging mode at the one or more lightsheet imaging positions on the basis of the position information. a) providing operating instructions causing the arrangement to be operated in the transmitted light imaging mode, b) selecting one or more regions of interest in the sample region based on selection information obtained on the basis of the stack of widefield images generated in the transmitted light imaging mode, c) providing position information relating to the one or more regions of interest selected in the sample region, and d) providing operating instructions causing the arrangement to be operated in the lightsheet imaging mode at the one or more lightsheet imaging positions on the basis of the position information.