A dispensing method which dispenses a first liquid contained in a first vessel which stores the first liquid and a second liquid not miscible with the first liquid and having lower specific gravity than that of the first liquid so as to introduce the first liquid into a second vessel by using a tube, comprising: a second suction step which sucks the first liquid contained in the first vessel to introduce this first liquid into the tube through the one end of the tube; and a third suction step which sucks the second liquid contained in the first vessel to introduce this second liquid into the tube through the one end of the tube; characterized in that the second vessel stores a third liquid not miscible with the first liquid and miscible with the second liquid, wherein the method further comprises: a first suction step which sucks the second liquid or the third liquid to introduce this second or third liquid into the tube through one end of the tube; a first discharge step which releases the second liquid sucked into the tube in the third suction step to discharge this second liquid into the second vessel; a second discharge step which releases the first liquid sucked into the tube in the second suction step to discharge this first liquid into the second vessel; and a third discharge step which releases at least a part of the second liquid or the third liquid sucked into the tube in the first suction step to discharge this second or third liquid into the second vessel. a second suction step which sucks the first liquid contained in the first vessel to introduce this first liquid into the tube through the one end of the tube; and a third suction step which sucks the second liquid contained in the first vessel to introduce this second liquid into the tube through the one end of the tube; characterized in that the second vessel stores a third liquid not miscible with the first liquid and miscible with the second liquid, wherein the method further comprises: a first suction step which sucks the second liquid or the third liquid to introduce this second or third liquid into the tube through one end of the tube; a first discharge step which releases the second liquid sucked into the tube in the third suction step to discharge this second liquid into the second vessel; a second discharge step which releases the first liquid sucked into the tube in the second suction step to discharge this first liquid into the second vessel; and a third discharge step which releases at least a part of the second liquid or the third liquid sucked into the tube in the first suction step to discharge this second or third liquid into the second vessel. a first suction step which sucks the second liquid or the third liquid to introduce this second or third liquid into the tube through one end of the tube; a first discharge step which releases the second liquid sucked into the tube in the third suction step to discharge this second liquid into the second vessel; a second discharge step which releases the first liquid sucked into the tube in the second suction step to discharge this first liquid into the second vessel; and a third discharge step which releases at least a part of the second liquid or the third liquid sucked into the tube in the first suction step to discharge this second or third liquid into the second vessel.