A cartridge configured for purifying a hybridized target molecule sample and imaging a hybridized target molecule, comprising: a sample input area configured to hold a target molecule sample, the sample comprising: a plurality of hybridized complexes comprising a plurality of target molecules each hybridized with a first probe and a second probe, a plurality of non-hybridized first probes, and a plurality of non-hybridized second probes; a first binding chamber configured to receive and/or contain a first affinity matrix and to receive the sample, wherein: the first affinity matrix is functionalized with first molecules configured to bind with the non-hybridized first probes and hybridized complexes of the sample during a first period of time; the first binding chamber is additionally configured to receive a first buffer to remove non-hybridized second probes from the sample after the non-hybridized first probes and hybridized complexes of the sample bind with the first affinity matrix; a first elution channel operatively configured to a first heater, and configured to receive the first affinity matrix after the first period of time and configured for heating the first affinity matrix to elute a first eluted sample comprising the plurality of hybridized complexes and plurality of non-hybridized first probes; a second binding chamber configured to receive and/or contain a second affinity matrix and to receive the first eluted sample, wherein: the second affinity matrix is functionalized with second molecules configured to bind with the hybridized complexes during a second period of time; the second binding chamber is additionally configured to receive a second buffer to remove at least non-hybridized first probes; a second elution channel operatively configured to a second heater, and configured to receive the second affinity matrix after the second period of time and configured for heating the second affinity matrix to elute a second eluted sample comprising the plurality of hybridized complexes; and a binding area having an active binding surface configured to receive the second eluted sample and bind with the hybridized complexes. a sample input area configured to hold a target molecule sample, the sample comprising: a plurality of hybridized complexes comprising a plurality of target molecules each hybridized with a first probe and a second probe, a plurality of non-hybridized first probes, and a plurality of non-hybridized second probes; a plurality of hybridized complexes comprising a plurality of target molecules each hybridized with a first probe and a second probe, a plurality of non-hybridized first probes, and a plurality of non-hybridized second probes; a first binding chamber configured to receive and/or contain a first affinity matrix and to receive the sample, wherein: the first affinity matrix is functionalized with first molecules configured to bind with the non-hybridized first probes and hybridized complexes of the sample during a first period of time; the first binding chamber is additionally configured to receive a first buffer to remove non-hybridized second probes from the sample after the non-hybridized first probes and hybridized complexes of the sample bind with the first affinity matrix; a first elution channel operatively configured to a first heater, and configured to receive the first affinity matrix after the first period of time and configured for heating the first affinity matrix to elute a first eluted sample comprising the plurality of hybridized complexes and plurality of non-hybridized first probes; a second binding chamber configured to receive and/or contain a second affinity matrix and to receive the first eluted sample, wherein: the second affinity matrix is functionalized with second molecules configured to bind with the hybridized complexes during a second period of time; the second binding chamber is additionally configured to receive a second buffer to remove at least non-hybridized first probes; a second elution channel operatively configured to a second heater, and configured to receive the second affinity matrix after the second period of time and configured for heating the second affinity matrix to elute a second eluted sample comprising the plurality of hybridized complexes; and a binding area having an active binding surface configured to receive the second eluted sample and bind with the hybridized complexes.