Method for light sheet microscopy, comprising the following method steps: - scanning a sample volume to be imaged using illumination light formed into a light sheet, which includes an angle δ ≠ 90° with the optical axis of an objective, wherein - the light sheet is aligned into the entire sample volume to be imaged in a propagation direction, - the illumination and the detection are implemented jointly through the objective, - the illumination light is shone into the entrance pupil of the objective at an entrance location offset parallel to the optical axis of said objective, wherein the angle δ is specified by the off-centre incoming radiation, and wherein - the depth of field S obj of the objective is less than the depth T of this sample volume in the direction of the optical axis, characterized in that - use is made of a device designed to - change a distance of the entrance location from the optical axis, - displace the entrance location on the circumference of the divided circle and - change the direction of the incoming illumination light; - a distance of the entrance location from the optical axis of the joint objective is set; - a rotation of the light sheet about the optical axis of the joint objective is set by means of a displacement of the entrance location to the circumference of a divided circle in the entrance pupil, and - a lateral extent of the light sheet is set by virtue of an incoming radiation direction of the illumination light into the entrance pupil being changed, - the sample volume scanned by means of the light sheet is imaged as a whole on a receiver plane and a depth-resolved image is ascertained by calculation from the registered intensity distribution in the detection light beam, and - a two-dimensional or three-dimensional image of the scanned sample volume is created according to light-field technology, wherein use is made of a microlens array in the detection beam path and a respective current illumination geometry is included for the three-dimensional representation of the scanned sample volume. - scanning a sample volume to be imaged using illumination light formed into a light sheet, which includes an angle δ ≠ 90° with the optical axis of an objective, wherein - the light sheet is aligned into the entire sample volume to be imaged in a propagation direction, - the illumination and the detection are implemented jointly through the objective, - the illumination light is shone into the entrance pupil of the objective at an entrance location offset parallel to the optical axis of said objective, wherein the angle δ is specified by the off-centre incoming radiation, and wherein - the light sheet is aligned into the entire sample volume to be imaged in a propagation direction, - the illumination and the detection are implemented jointly through the objective, - the illumination light is shone into the entrance pupil of the objective at an entrance location offset parallel to the optical axis of said objective, wherein the angle δ is specified by the off-centre incoming radiation, and wherein - the depth of field S obj of the objective is less than the depth T of this sample volume in the direction of the optical axis, characterized in that - use is made of a device designed to - change a distance of the entrance location from the optical axis, - displace the entrance location on the circumference of the divided circle and - change the direction of the incoming illumination light; - change a distance of the entrance location from the optical axis, - displace the entrance location on the circumference of the divided circle and - change the direction of the incoming illumination light; - a distance of the entrance location from the optical axis of the joint objective is set; - a rotation of the light sheet about the optical axis of the joint objective is set by means of a displacement of the entrance location to the circumference of a divided circle in the entrance pupil, and - a lateral extent of the light sheet is set by virtue of an incoming radiation direction of the illumination light into the entrance pupil being changed, - the sample volume scanned by means of the light sheet is imaged as a whole on a receiver plane and a depth-resolved image is ascertained by calculation from the registered intensity distribution in the detection light beam, and - a two-dimensional or three-dimensional image of the scanned sample volume is created according to light-field technology, wherein use is made of a microlens array in the detection beam path and a respective current illumination geometry is included for the three-dimensional representation of the scanned sample volume.