A protease variant comprising a modification at one or more positions corresponding to positions 39, 50, 57, 60, 74, 81, 84, 109, 110, 111, 115, 117, 124, 142, 145, 146, 154, 182, 183, 187, 207, 209, 210, 228, 267, 271, 272, 274, 280, 294, 317, 318, 320, 321, 322, 328, 343, 348, 362 or 363 of the polypeptide of SEQ ID NO: 3, wherein the variant has protease activity and wherein the variant has at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the mature polypeptide of SEQ ID NO: 3, and wherein the variant has increased thermo-stability compared to the protease of SEQ ID NO: 3, wherein the increased thermo-stability is increased residual activity measured after incubation for 30 min at a temperature in the range from 55 to 60 degrees Celsius, and wherein the variant comprises at least one of the following modifications or combination of modifications: N115L; S183P; N115L + Q182G; N115L + Q182R; E81R + S183P; E81K + S183P; S183P + Q154V; S183P + Q142W; Q142R + S183P; S183P + T146A; S183P + T146W; S183P + I228R; S183P + D267N; S183P + S272V; S183P + S272R; T146Y + S183P; S183P + S294A; S183P + T362A; S183P + S294A; S183P + E74W; S183P + E81A; N115L + S183L + S187L; S183L + V209L + S210 deletion; D109P + V209L + S210 deletion; N115D + V209L + S210 deletion; E81R + V209L + S210 deletion; D109P + V209L + S210 deletion; N115D + V209L + S210 deletion; E81R + V209L + S210 deletion; T146W + S183P + D280N; I84C + S183P + S272C; I39M + Q142R + S183P; I39R + Q142R + S183P; I39L + Q142R + S183P; I39C + Q142R + S183P; E117D + Q142R + S183P; S60D + Q142R + S183P; N115L + S183L +S187L + P348A; D109P + S183P + V209L + S210 deletion; N115D + S183P + V209L + S210 deletion; E81R + S183P + V209L +S210 deletion; V209L + S210 deletion + S317A + S318 deletion; Q142R + N145G + T146E + S183P; Q142R + N145Q + T146D + S183P; Q142R + N145V + T146E + S183P; Q142R + N145D + T146E + S183P; Q142R + N145K + T146E + S183P; Q142R + N145A + T146D +S183P; Q142R + N145E + T146E + S183P; N115L + S183L + S187L + V209W + S210 deletion; N115L + S183L + S187L + V209L + S210 deletion; N115L + S183L + S187L + S317G + S318 deletion; N115L + S183L + S187L + S317S + S318 deletion; N115L +S183L + S187L + S317A + S318 deletion; E81R + V209L + S210 deletion + S317A + S318 deletion. N115L; S183P; N115L + Q182G; N115L + Q182R; E81R + S183P; E81K + S183P; S183P + Q154V; S183P + Q142W; Q142R + S183P; S183P + T146A; S183P + T146W; S183P + I228R; S183P + D267N; S183P + S272V; S183P + S272R; T146Y + S183P; S183P + S294A; S183P + T362A; S183P + S294A; S183P + E74W; S183P + E81A; N115L + S183L + S187L; S183L + V209L + S210 deletion; D109P + V209L + S210 deletion; N115D + V209L + S210 deletion; E81R + V209L + S210 deletion; D109P + V209L + S210 deletion; N115D + V209L + S210 deletion; E81R + V209L + S210 deletion; T146W + S183P + D280N; I84C + S183P + S272C; I39M + Q142R + S183P; I39R + Q142R + S183P; I39L + Q142R + S183P; I39C + Q142R + S183P; E117D + Q142R + S183P; S60D + Q142R + S183P; N115L + S183L +S187L + P348A; D109P + S183P + V209L + S210 deletion; N115D + S183P + V209L + S210 deletion; E81R + S183P + V209L +S210 deletion; V209L + S210 deletion + S317A + S318 deletion; Q142R + N145G + T146E + S183P; Q142R + N145Q + T146D + S183P; Q142R + N145V + T146E + S183P; Q142R + N145D + T146E + S183P; Q142R + N145K + T146E + S183P; Q142R + N145A + T146D +S183P; Q142R + N145E + T146E + S183P; N115L + S183L + S187L + V209W + S210 deletion; N115L + S183L + S187L + V209L + S210 deletion; N115L + S183L + S187L + S317G + S318 deletion; N115L + S183L + S187L + S317S + S318 deletion; N115L +S183L + S187L + S317A + S318 deletion; E81R + V209L + S210 deletion + S317A + S318 deletion.