A physiological signal monitoring device for sensing a physiological signal in an analyte of a host, comprising: a sensing member, including a signal sensing end adapted to be inserted underneath a skin of the host to sense the physiological signal, and a signal output end for outputting the physiological signal; and a transmitter connected to said sensing member for receiving, processing and transmitting the physiological signal, said physiological signal monitoring device characterized in that said transmitter includes a circuit board having a plurality of electrical contacts, and a connecting port connected to said circuit board and having a socket which is communicated to said circuit board, a port casing which is mounted on said circuit board and which is formed with said socket, and a plurality of conducting springs which are disposed at two opposite sides of said socket, wherein said port casing has a plurality of slanted surfaces facing said circuit board and said sensing member thereby forcing said conducting springs against said circuit board and said sensing member; wherein, said sensing member is removably inserted into said socket; wherein, each of said conducting springs has one side electrically connected to a respective one of said electrical contacts of said circuit board and another side electrically connected to said signal output end of said sensing member for electric connection between the respective one of said electrical contacts and said signal output end; and wherein, each of said conducting springs is frictionally rotated by said sensing member during insertion of said sensing member into said socket and removal of said sensing member from said socket. a sensing member, including a signal sensing end adapted to be inserted underneath a skin of the host to sense the physiological signal, and a signal output end for outputting the physiological signal; and a signal sensing end adapted to be inserted underneath a skin of the host to sense the physiological signal, and a signal output end for outputting the physiological signal; and a transmitter connected to said sensing member for receiving, processing and transmitting the physiological signal, said physiological signal monitoring device characterized in that said transmitter includes a circuit board having a plurality of electrical contacts, and a connecting port connected to said circuit board and having a socket which is communicated to said circuit board, a port casing which is mounted on said circuit board and which is formed with said socket, and a plurality of conducting springs which are disposed at two opposite sides of said socket, wherein said port casing has a plurality of slanted surfaces facing said circuit board and said sensing member thereby forcing said conducting springs against said circuit board and said sensing member; wherein, said sensing member is removably inserted into said socket; wherein, each of said conducting springs has one side electrically connected to a respective one of said electrical contacts of said circuit board and another side electrically connected to said signal output end of said sensing member for electric connection between the respective one of said electrical contacts and said signal output end; and wherein, each of said conducting springs is frictionally rotated by said sensing member during insertion of said sensing member into said socket and removal of said sensing member from said socket.