A column assembly for chromatography, the column assembly comprising: a column jacket tube and a column liner tube configured for use as a column, wherein the column jacket tube comprises a first portion having a first outside diameter, a first metal, and a first inner diameter, the column jacket tube further comprising an end portion at one end configured to removably receive a retainer body enabled for fluid communication with the column liner tube, wherein the end portion of the one end comprises a first diameter, the column jacket tube further comprising a threaded tube portion at one end of the column jacket tube, the threaded tube portion configured to threadingly engage with the retainer body, wherein the threaded tube portion comprises: a second diameter that is greater than the first diameter; and a shoulder that detains the retainer body when the threaded tube portion is engaged with the retainer body, wherein the shoulder is positioned between the second diameter of the threaded tube portion and the first diameter of the end portion at the one end; the column liner tube being configured to cylindrically cover the first inner diameter of the column jacket tube and having a second inner diameter less than the first inner diameter, wherein the second inner diameter defines a column passageway enabled to receive a stationary phase and a mobile phase, and to isolate at least the mobile phase from contact with the column jacket tube, wherein the column liner tube is formed from a biocompatible material and the column liner tube further integrally comprises a flange portion that radially covers the one end of the column jacket tube; and a removeable frit retainer configured to mate with the shoulder at a first end and to mate with a second cavity in the retainer body at a second end, the frit retainer comprising: a first cavity at the first end configured to receive the end of the column jacket tube radially covered by the flange portion of the column liner tube, a central opening for removably receiving the end portion, a passageway therethrough, and a third cavity configured to removably receive and hold a frit, wherein, when the threaded tube portion threadingly engages with the retainer body, the flange portion engages with a portion of the removeable frit retainer to fluidically seal the column liner tube against the first cavity, wherein the removeable frit retainer is enclosed by the retainer body. a column jacket tube and a column liner tube configured for use as a column, wherein the column jacket tube comprises a first portion having a first outside diameter, a first metal, and a first inner diameter, the column jacket tube further comprising an end portion at one end configured to removably receive a retainer body enabled for fluid communication with the column liner tube, wherein the end portion of the one end comprises a first diameter, the column jacket tube further comprising a threaded tube portion at one end of the column jacket tube, the threaded tube portion configured to threadingly engage with the retainer body, wherein the threaded tube portion comprises: a second diameter that is greater than the first diameter; and a shoulder that detains the retainer body when the threaded tube portion is engaged with the retainer body, wherein the shoulder is positioned between the second diameter of the threaded tube portion and the first diameter of the end portion at the one end; a second diameter that is greater than the first diameter; and a shoulder that detains the retainer body when the threaded tube portion is engaged with the retainer body, wherein the shoulder is positioned between the second diameter of the threaded tube portion and the first diameter of the end portion at the one end; the column liner tube being configured to cylindrically cover the first inner diameter of the column jacket tube and having a second inner diameter less than the first inner diameter, wherein the second inner diameter defines a column passageway enabled to receive a stationary phase and a mobile phase, and to isolate at least the mobile phase from contact with the column jacket tube, wherein the column liner tube is formed from a biocompatible material and the column liner tube further integrally comprises a flange portion that radially covers the one end of the column jacket tube; and a removeable frit retainer configured to mate with the shoulder at a first end and to mate with a second cavity in the retainer body at a second end, the frit retainer comprising: a first cavity at the first end configured to receive the end of the column jacket tube radially covered by the flange portion of the column liner tube, a central opening for removably receiving the end portion, a passageway therethrough, and a third cavity configured to removably receive and hold a frit, a first cavity at the first end configured to receive the end of the column jacket tube radially covered by the flange portion of the column liner tube, a central opening for removably receiving the end portion, a passageway therethrough, and a third cavity configured to removably receive and hold a frit, wherein, when the threaded tube portion threadingly engages with the retainer body, the flange portion engages with a portion of the removeable frit retainer to fluidically seal the column liner tube against the first cavity, wherein the removeable frit retainer is enclosed by the retainer body.