mhurhangee/ep-ind-claims huggingface dataset:
A method of making polypeptide-polymer conjugates having one or more altered pharmacological properties from a plurality of polypeptides having C-termini comprising a sortase recognition site comprising LPXTG (SEQ ID NO: 3), wherein X is any amino acid, the method comprising: a) contacting the plurality of polypeptides with a sortase and an initiator agent comprising a nucleophilic group under conditions that permit attachment of the initiator agent through an amide linkage to the C-terminus to form a plurality of macroinitiators; and b) incubating the plurality of macroinitiators with a monomer under conditions that permit free-radical polymerization to occur from the initiator agent to form polypeptide-polymer conjugates, such that at least about 25% of the polypeptides have a conjugated polymer initiated solely from the C-terminus, wherein the free-radical polymerization comprises at least one of atom transfer radical polymerization (ATRP) and reversible addition-fragmentation chain transfer (RAFT). a) contacting the plurality of polypeptides with a sortase and an initiator agent comprising a nucleophilic group under conditions that permit attachment of the initiator agent through an amide linkage to the C-terminus to form a plurality of macroinitiators; and b) incubating the plurality of macroinitiators with a monomer under conditions that permit free-radical polymerization to occur from the initiator agent to form polypeptide-polymer conjugates, such that at least about 25% of the polypeptides have a conjugated polymer initiated solely from the C-terminus,
A method comprising: a) providing a solid support that is a bead, wherein attached to each solid support is one or more protein ligands capable of specifically binding to an analyte and an addressable marker that is an oligonucleotide marker, wherein the addressable marker identifies the one or more protein ligand(s); b) contacting the solid support with a sample comprising the analyte; c) contacting the solid support with a selection moiety, wherein the selection moiety specifically recognizes the analyte, and thereby binds to the solid support; d) isolating the selection moiety; e) treating the selection moiety such that bound solid support is released into a medium; f) applying the medium to a plurality of field effect transistors (FETs); g) detecting the electrical properties of the plurality of FETs and thereby detecting the presence of the analyte, wherein detecting the electrical properties comprises detecting electrical charge changes by at least one FET and thereby sequencing the oligonucleotide marker. a) providing a solid support that is a bead, wherein attached to each solid support is one or more protein ligands capable of specifically binding to an analyte and an addressable marker that is an oligonucleotide marker, wherein the addressable marker identifies the one or more protein ligand(s); b) contacting the solid support with a sample comprising the analyte; c) contacting the solid support with a selection moiety, wherein the selection moiety specifically recognizes the analyte, and thereby binds to the solid support; d) isolating the selection moiety; e) treating the selection moiety such that bound solid support is released into a medium; f) applying the medium to a plurality of field effect transistors (FETs); g) detecting the electrical properties of the plurality of FETs and thereby detecting the presence of the analyte, wherein detecting the electrical properties comprises detecting electrical charge changes by at least one FET and thereby sequencing the oligonucleotide marker.
Hot-air cooker comprising: - a housing including an internal ventilation conduit provided with an air inlet and an air outlet; - an outer container, installed inside the housing; - an upper cover, installed above the outer container; the outer container and the upper cover constituting a cavity, the air inlet and air outlet of the ventilation conduit communicating with the cavity; - an inner container, located inside the cavity; - a fan, installed inside the ventilation conduit; - a resistor, installed inside the ventilation conduit and adjacent to the fan, - a rotating mixer installed in the bottom of the inner container, - a motor installed at the bottom of the outer container, and of which the output shaft drives the mixer, characterised in that the cooker includes a heating plate located inside the cavity and through which passes the output shaft of the motor, the inner container being placed on the heating plate, and the food able to be heated by the heating plate and the resistor, at the same time or independently. - a housing including an internal ventilation conduit provided with an air inlet and an air outlet; - an outer container, installed inside the housing; - an upper cover, installed above the outer container; the outer container and the upper cover constituting a cavity, the air inlet and air outlet of the ventilation conduit communicating with the cavity; - an inner container, located inside the cavity; - a fan, installed inside the ventilation conduit; - a resistor, installed inside the ventilation conduit and adjacent to the fan, - a rotating mixer installed in the bottom of the inner container, - a motor installed at the bottom of the outer container, and of which the output shaft drives the mixer,
A method of reducing the sorbate concentration of a process fluid stream using a sorption bed system comprising a rotating mass of a regenerable sorbent material, the method comprising the steps of: rotating the sorbent mass so that, in a cycle of operation, a given volume of the sorbent mass sequentially passes through first, second, third, fourth, and fifth zones, before returning to the first zone; passing a process fluid stream through the sorbent mass in the first zone; passing a regeneration fluid stream through the sorbent mass in the third zone; and recycling an isolation fluid stream in a closed loop, independent of the process fluid stream and the regeneration fluid stream, between the sorbent mass in the fourth zone and in the second zone, wherein the regeneration fluid stream passes through the fifth zone before passing through the third zone. rotating the sorbent mass so that, in a cycle of operation, a given volume of the sorbent mass sequentially passes through first, second, third, fourth, and fifth zones, before returning to the first zone; passing a process fluid stream through the sorbent mass in the first zone; passing a regeneration fluid stream through the sorbent mass in the third zone; and recycling an isolation fluid stream in a closed loop, independent of the process fluid stream and the regeneration fluid stream, between the sorbent mass in the fourth zone and in the second zone, wherein the regeneration fluid stream passes through the fifth zone before passing through the third zone.
Filter system for a water-conducting household appliance, comprising: a main filter, which is arranged in a main line and is designed to filter particles out from a fluid, a bypass line, which is designed to guide fluid around the main filter, and an auxiliary filter, which is designed to filter particles out from fluid which is passed around the main filter through the bypass line, wherein the main filter and the auxiliary filter have the same pore width, and wherein the bypass line is designed to be activated and/or deactivated passively. a main filter, which is arranged in a main line and is designed to filter particles out from a fluid, a bypass line, which is designed to guide fluid around the main filter, and an auxiliary filter, which is designed to filter particles out from fluid which is passed around the main filter through the bypass line, wherein the main filter and the auxiliary filter have the same pore width, and wherein the bypass line is designed to be activated and/or deactivated passively.
Rear-view mirror unit including a housing for attachment to the vehicle, a mirror having a semitransparent area, and a camera positioned behind the mirror, characterized in that the camera is mounted linearly movably within the rear view mirror unit.
A compact microscope comprising: an enclosure (11; 205), a support element (12; 201), a primary optical support element (16; 202) located within the enclosure (11; 205) and supported by the support element (12; 201), at least one vibration isolating mount (15; 203) between the support element (12; 201) and the primary optical support element (16; 202), a moveable sample stage (19; 304) and an objective lens system (18; 301) mounted on the primary optical support element (16; 202) via a baseplate, the moveable sample stage (19; 304) being connected to the baseplate by a plate, the baseplate being mounted on the primary optical support element, a sample holder (20; 305) mounted on a face of the moveable sample stage (19; 304), and a return optical system to receive returned light from the moveable sample stage (19; 304) and transmit returned light to a detection apparatus, wherein the return optical system is mounted on the primary optical support element (16; 202), and wherein the moveable sample stage (19; 304) is connected to the baseplate via the plate by a fixed mounting point at the face of the moveable sample stage (19; 304) on which the sample holder (20; 305) is mounted and by a slidable mounting point remote from the face of the moveable sample stage (19; 304), the mounting points connecting the plate and the baseplate. an enclosure (11; 205), a support element (12; 201), a primary optical support element (16; 202) located within the enclosure (11; 205) and supported by the support element (12; 201), at least one vibration isolating mount (15; 203) between the support element (12; 201) and the primary optical support element (16; 202), a moveable sample stage (19; 304) and an objective lens system (18; 301) mounted on the primary optical support element (16; 202) via a baseplate, the moveable sample stage (19; 304) being connected to the baseplate by a plate, the baseplate being mounted on the primary optical support element, a sample holder (20; 305) mounted on a face of the moveable sample stage (19; 304), and a return optical system to receive returned light from the moveable sample stage (19; 304) and transmit returned light to a detection apparatus, wherein the return optical system is mounted on the primary optical support element (16; 202), and wherein the moveable sample stage (19; 304) is connected to the baseplate via the plate by a fixed mounting point at the face of the moveable sample stage (19; 304) on which the sample holder (20; 305) is mounted and by a slidable mounting point remote from the face of the moveable sample stage (19; 304), the mounting points connecting the plate and the baseplate.
A method for generating a starter culture comprising at least two different bacteriophage resistant variant strains, comprising the steps of: (a) exposing a parent bacterial strain comprising at least a portion of a CRISPR locus to at least one bacteriophage to produce a mixture of bacteria comprising at least one bacteriophage resistant variant strain comprising a modified CRISPR locus comprising at least one additional spacer in said modified CRISPR locus; (b) exposing a parent bacterial strain comprising at least a portion of a CRISPR locus to at least one bacteriophage to produce a mixture of bacteria comprising at least another bacteriophage resistant variant strain comprising a modified CRISPR locus comprising at least one additional spacer in said modified CRISPR locus; (c) selecting said bacteriophage resistant variant strains from said mixtures of bacteria; (d) selecting said bacteriophage resistant variant strains comprising an additional spacer in said modified CRISPR locus from said bacteriophage resistant strains selected in step (c); and (e) isolating said bacteriophage resistant variant strains, wherein said strains comprise an additional spacer in said modified CRISPR locus. (a) exposing a parent bacterial strain comprising at least a portion of a CRISPR locus to at least one bacteriophage to produce a mixture of bacteria comprising at least one bacteriophage resistant variant strain comprising a modified CRISPR locus comprising at least one additional spacer in said modified CRISPR locus; (b) exposing a parent bacterial strain comprising at least a portion of a CRISPR locus to at least one bacteriophage to produce a mixture of bacteria comprising at least another bacteriophage resistant variant strain comprising a modified CRISPR locus comprising at least one additional spacer in said modified CRISPR locus; (c) selecting said bacteriophage resistant variant strains from said mixtures of bacteria; (d) selecting said bacteriophage resistant variant strains comprising an additional spacer in said modified CRISPR locus from said bacteriophage resistant strains selected in step (c); and (e) isolating said bacteriophage resistant variant strains, wherein said strains comprise an additional spacer in said modified CRISPR locus.
Injectable co-crosslinked cohesive gel comprising at least a first highly crosslinked polysaccharide gel having a crosslinking rate X1' of between 0.21 and 0.9 in the form of homogeneously distributed particles in at least a second slightly crosslinked polysaccharide gel having a crosslinking rate X2 of between 0.03 and 0.3, said at least first gel being bound by covalent bonds to said at least second gel, the crosslinking rate of said highly crosslinked polysaccharide being greater than the crosslinking rate of said slightly crosslinked polysaccharide in which: - said highly crosslinked polysaccharide and said slightly crosslinked polysaccharide are selected from hyaluronic acid or a salt thereof, chondroitin sulfate or a salt thereof and hydroxypropylmethylcellulose and at least one of said polysaccharides is a hyaluronic acid or a salt thereof, - the crosslinking rates X1' and X2 are defined as the ratio: X = number of moles of crosslinking agent introduced into the reaction medium total number of disaccharide units introduced into the reaction medium - the first highly crosslinked gel is in the form of homogeneously distributed particles in the second gel; - said highly crosslinked polysaccharide particles are covalently bonded to the slightly crosslinked polysaccharide; - the weight proportion of said highly crosslinked polysaccharide particles having a crosslinking rate X1' of between 0.21 and 0.9 is between 0.5 to 99%; - the size of said particles is between 10 μm and 5 mm. - said highly crosslinked polysaccharide and said slightly crosslinked polysaccharide are selected from hyaluronic acid or a salt thereof, chondroitin sulfate or a salt thereof and hydroxypropylmethylcellulose and at least one of said polysaccharides is a hyaluronic acid or a salt thereof, - the crosslinking rates X1' and X2 are defined as the ratio: X = number of moles of crosslinking agent introduced into the reaction medium total number of disaccharide units introduced into the reaction medium - the first highly crosslinked gel is in the form of homogeneously distributed particles in the second gel; - said highly crosslinked polysaccharide particles are covalently bonded to the slightly crosslinked polysaccharide; - the weight proportion of said highly crosslinked polysaccharide particles having a crosslinking rate X1' of between 0.21 and 0.9 is between 0.5 to 99%; - the size of said particles is between 10 μm and 5 mm.
A delivery device of a substance in the form of aerosol, apt to be used in combination with a container of the substance in liquid form and with manual actuation means apt to expel the substance from the container, in particular in combination with a syringe, which delivery device comprises: - connection means, apt to connect the device with the container; - an elongated outer body bearing a nozzle for dispensing the substance in the form of an aerosol; - an inner body, received within said outer body; and - a fluid-dynamic path comprised between said connection means and said dispensing nozzle and defined between said outer body and said inner body, which path is configured such that, in use, a manual actuation of said actuation means produces the input of the substance in liquid form in said fluid-dynamic path through said connection means and the outlet of the substance in the form of an aerosol through said dispensing nozzle, which fluid-dynamic path comprises ▪ an expansion chamber, arranged immediately downstream to said connection means with respect to the flow of the substance within the device and having a substantially annular geometry; ▪ an acceleration chamber, arranged immediately downstream to said expansion chamber with respect to the flow of the substance within the device and having a substantially elongated geometry, which acceleration chamber develops according to a longitudinal direction substantially parallel to an axis of said expansion annular chamber and/or to a longitudinal axis of the device. - connection means, apt to connect the device with the container; - an elongated outer body bearing a nozzle for dispensing the substance in the form of an aerosol; - an inner body, received within said outer body; and - a fluid-dynamic path comprised between said connection means and said dispensing nozzle and defined between said outer body and said inner body, which path is configured such that, in use, a manual actuation of said actuation means produces the input of the substance in liquid form in said fluid-dynamic path through said connection means and the outlet of the substance in the form of an aerosol through said dispensing nozzle, ▪ an expansion chamber, arranged immediately downstream to said connection means with respect to the flow of the substance within the device and having a substantially annular geometry; ▪ an acceleration chamber, arranged immediately downstream to said expansion chamber with respect to the flow of the substance within the device and having a substantially elongated geometry, which acceleration chamber develops according to a longitudinal direction substantially parallel to an axis of said expansion annular chamber and/or to a longitudinal axis of the device.
A variable outer air seal support system, comprising: a case having plurality of slots; an extension of a variable outer air seal segment, the extension providing at least one extension aperture; a connector pin extending through both one of the plurality of slots and the at least one extension aperture, the connector pin configured to move within the slot to move the variable outer air seal segment from a first position to a second position, the variable outer air seal segment overlapping a circumferentially adjacent variable outer air seal segment more in the first position than in the second position; and a link having a first end and a second end that is opposite the first end, the first end providing at least one link aperture that receives the connector pin, characterised in that the second end is configured to engage another connector pin associated with a circumferentially adjacent variable outer air seal segment. a case having plurality of slots; an extension of a variable outer air seal segment, the extension providing at least one extension aperture; a connector pin extending through both one of the plurality of slots and the at least one extension aperture, the connector pin configured to move within the slot to move the variable outer air seal segment from a first position to a second position, the variable outer air seal segment overlapping a circumferentially adjacent variable outer air seal segment more in the first position than in the second position; and a link having a first end and a second end that is opposite the first end, the first end providing at least one link aperture that receives the connector pin, characterised in that the second end is configured to engage another connector pin associated with a circumferentially adjacent variable outer air seal segment.
A package carton for receiving a package of cigarette packs, the package carton comprising first and second part packs (TP1, TP2), and the first part pack including a tab and the second part pack including a slot for receiving the tab, wherein the tab is adapted to be detached out of a wall of the first part pack by means of a partly circumscriptive separating line and extends away from a first edge of the first part pack and is adapted to be deflected out of the wall about the first edge, the tab still being hinged to the wall along the first edge, the slot is likewise located on a first edge of the second part pack, the first edge of the first part pack and the first edge of the second part pack come to rest against each other to form the package carton such that the tab in the deflected-out condition can be inserted into the slot for a hinged connection of the two part packs (TP1, TP2), the tab includes lateral shoulders (ABS1, ABS2) which restrain the tab inserted in the slot such that the first part pack and the second part pack are held against each other along their first edges (K11, K12) and can be swiveled relative to each other about the first edges (K11, K12), characterized in that the tab includes an intermediate portion and a head portion, the intermediate portion being designed to hingedly couple the head portion to the first part pack, and wherein the length of the intermediate portion is equal to or greater than half the thickness of the wall of the second part pack and equal to or smaller than five times the thickness of the wall of the second part pack.
A device for the detachable and hooked-in connection of at least two adjacently arranged pieces of furniture (1, 2) in a geometric plane, wherein the device has a stationary combination journal, a connection lug and a locking element, - wherein each of the pieces of furniture (1, 2) to be connected supports the connection lug on the stationary combination journal, which connection lug can be pivoted thereon and latches in at least one position, - wherein the connection lug has a hook-in cut-out at the free end thereof, - wherein the combination journal has a bearing pin, - wherein the combination journal of a first piece of furniture is hooked into the hook-in cut-out of the connection lug of a second piece of furniture or is hooked in and locked by means of a locking element, - characterised in that the locking element is a pivot knob which is pivotably and latchably mounted eccentrically on the combination journal. - wherein each of the pieces of furniture (1, 2) to be connected supports the connection lug on the stationary combination journal, which connection lug can be pivoted thereon and latches in at least one position, - wherein the connection lug has a hook-in cut-out at the free end thereof, - wherein the combination journal has a bearing pin, - wherein the combination journal of a first piece of furniture is hooked into the hook-in cut-out of the connection lug of a second piece of furniture or is hooked in and locked by means of a locking element, - characterised in that the locking element is a pivot knob which is pivotably and latchably mounted eccentrically on the combination journal.
A vibrating apparatus for attachment to a construction machine, in particular to a leader of a construction machine, comprising: an excitation cell for generating a vibration movement; a connector for a drain of lubricant of the excitation cell; a connector for a supply of lubricant into the excitation cell; and a pump for conveying lubricant between the connector for a drain and the connector for a supply of lubricant, characterized in that the pump is drivable via a separate line, preferably a pressure line. an excitation cell for generating a vibration movement; a connector for a drain of lubricant of the excitation cell; a connector for a supply of lubricant into the excitation cell; and a pump for conveying lubricant between the connector for a drain and the connector for a supply of lubricant, characterized in that the pump is drivable via a separate line, preferably a pressure line.