A tissue removal device comprising: a tube with a predetermined length, an operation unit connected with one side of the tube, a first wire which is located inside of the tube and has one side connected with the operation unit, a first incision knife which is connected with the other side of the first wire, wherein at least a part of the first incision knife can be drawn out to an outside of the tube, a ball which is positioned outside of the tube and is connected to a tip of the first incision knife, a second wire which is disposed inside of the tube and has one side connected with the operation unit, and a second incision knife which is connected with the other side of the second wire, penetrates through the first incision knife, and can be protruded from the ball, wherein the first incision knife is movable with the first wire by operation of the operation unit, wherein the operation unit comprises a body connected with the tube and having an inside being empty, a rail connected with the body, and a slide movably connected with the rail and connected with the first wire, wherein one side of the second wire is drawn out of the body, wherein a first inlet connected with the inside of the body is formed on a surface of the body, and the inside of the body is connected with the inside of the tube, an injection body connected with the one side of the second wire drawn out of the operation unit and having a second inlet, wherein pathways (31, 43a) are formed inside of the second wire and inside of the second incision knife and are connected with each other, and the pathway of the second wire is connected with the second inlet. a tube with a predetermined length, an operation unit connected with one side of the tube, a first wire which is located inside of the tube and has one side connected with the operation unit, a first incision knife which is connected with the other side of the first wire, wherein at least a part of the first incision knife can be drawn out to an outside of the tube, a ball which is positioned outside of the tube and is connected to a tip of the first incision knife, a second wire which is disposed inside of the tube and has one side connected with the operation unit, and a second incision knife which is connected with the other side of the second wire, penetrates through the first incision knife, and can be protruded from the ball, wherein the first incision knife is movable with the first wire by operation of the operation unit, wherein the operation unit comprises a body connected with the tube and having an inside being empty, a rail connected with the body, and a slide movably connected with the rail and connected with the first wire, wherein one side of the second wire is drawn out of the body, wherein a first inlet connected with the inside of the body is formed on a surface of the body, and the inside of the body is connected with the inside of the tube, an injection body connected with the one side of the second wire drawn out of the operation unit and having a second inlet, wherein pathways (31, 43a) are formed inside of the second wire and inside of the second incision knife and are connected with each other, and the pathway of the second wire is connected with the second inlet.