A target detection system comprising: (a) a first nucleic acid probe that comprises a (i) 5' Z double-stranded domain comprising a 5' primer-binding subdomain linked to a 3' barcode subdomain, wherein the 3' barcode subdomain is formed by base pairing between a sequence of nucleotides that lacks one of A, T, C or G and a complementary sequence of nucleotides, and (ii) a 3' single-stranded primer-binding domain, wherein a nucleotide corresponding to the nucleotide lacking from the 3' barcode subdomain is located between the 3' barcode subdomain and the 5' primer-binding subdomain, and wherein the 5' primer-binding subdomain comprises a 5' molecule that terminates polymerization; (b) a second nucleic acid probe that comprises a 5' primer-binding domain linked to a 3' primer domain that is complementary to the 3' single-stranded primer-binding domain of the first nucleic acid probe; (c) a primer that is complementary to the 5' primer-binding subdomain of the first nucleic acid probe; and (d) a primer that is complementary to the 5' primer-binding domain of the second nucleic acid probe, wherein the first nucleic acid probe is conjugated to a target-binding molecule and the second nucleic acid probe is conjugated to a target-binding molecule, and wherein the system further comprises a mixture of dNTPs that lacks one of dATP, dTTP, dCTP or dGTP, wherein the dNTP that is lacking corresponds to the complement of the nucleotide that is lacking from the 3' barcode subdomain of the first nucleic acid probe. (a) a first nucleic acid probe that comprises a (i) 5' Z double-stranded domain comprising a 5' primer-binding subdomain linked to a 3' barcode subdomain, wherein the 3' barcode subdomain is formed by base pairing between a sequence of nucleotides that lacks one of A, T, C or G and a complementary sequence of nucleotides, and (ii) a 3' single-stranded primer-binding domain, wherein a nucleotide corresponding to the nucleotide lacking from the 3' barcode subdomain is located between the 3' barcode subdomain and the 5' primer-binding subdomain, and wherein the 5' primer-binding subdomain comprises a 5' molecule that terminates polymerization; (i) 5' Z double-stranded domain comprising a 5' primer-binding subdomain linked to a 3' barcode subdomain, wherein the 3' barcode subdomain is formed by base pairing between a sequence of nucleotides that lacks one of A, T, C or G and a complementary sequence of nucleotides, and (ii) a 3' single-stranded primer-binding domain, wherein a nucleotide corresponding to the nucleotide lacking from the 3' barcode subdomain is located between the 3' barcode subdomain and the 5' primer-binding subdomain, and wherein the 5' primer-binding subdomain comprises a 5' molecule that terminates polymerization; (b) a second nucleic acid probe that comprises a 5' primer-binding domain linked to a 3' primer domain that is complementary to the 3' single-stranded primer-binding domain of the first nucleic acid probe; (c) a primer that is complementary to the 5' primer-binding subdomain of the first nucleic acid probe; and (d) a primer that is complementary to the 5' primer-binding domain of the second nucleic acid probe, wherein the first nucleic acid probe is conjugated to a target-binding molecule and the second nucleic acid probe is conjugated to a target-binding molecule, and wherein the system further comprises a mixture of dNTPs that lacks one of dATP, dTTP, dCTP or dGTP, wherein the dNTP that is lacking corresponds to the complement of the nucleotide that is lacking from the 3' barcode subdomain of the first nucleic acid probe.