A method for examining a microscopic sample arranged on a sample carrier with a magnifying digital-optical imaging device by which the sample is imaged by optical means and a digital image of the sample is generated, and for treating the microscopic sample arranged on the sample carrier with a laser microdissection system which comprises microscope optics and a laser unit and with which a laser beam is generated for treating the sample, comprising the following steps: a) generating at least two reference markings on the sample carrier with the laser beam of the laser microdissection system which can be identified both by means of the digital-optical imaging device and by means of the laser microdissection system, b) arranging the sample on the same sample carrier before or after the reference markings are created on the sample carrier according to the above step a), c) producing a digital image of the sample on the sample carrier with the digital-optical imaging device, wherein the image also comprises the reference markings, d) setting at least one image region of the image and generating first position information data indicating the position of the at least one image region in the image, e) identifying the reference markings in the image and generating second position information data indicating the position of the reference markings in the image before, during or after the at least one image region of the image is determined in accordance with the aforementioned step d), f) providing the first and second position information data to the laser microdissection system, g) imaging the sample carrier with the specimen and the reference markings and identifying the reference markings with the laser microdissection system, and generating third position information data indicating the position of the reference markings in the laser microdissection system, and h) correlating the first position information data, the second position information data and the third position information data, and processing at least one sample region of the sample corresponding to the at least one image region of the image defined in the aforementioned step d) with the laser microdissection system. a) generating at least two reference markings on the sample carrier with the laser beam of the laser microdissection system which can be identified both by means of the digital-optical imaging device and by means of the laser microdissection system, b) arranging the sample on the same sample carrier before or after the reference markings are created on the sample carrier according to the above step a), c) producing a digital image of the sample on the sample carrier with the digital-optical imaging device, wherein the image also comprises the reference markings, d) setting at least one image region of the image and generating first position information data indicating the position of the at least one image region in the image, e) identifying the reference markings in the image and generating second position information data indicating the position of the reference markings in the image before, during or after the at least one image region of the image is determined in accordance with the aforementioned step d), f) providing the first and second position information data to the laser microdissection system, g) imaging the sample carrier with the specimen and the reference markings and identifying the reference markings with the laser microdissection system, and generating third position information data indicating the position of the reference markings in the laser microdissection system, and h) correlating the first position information data, the second position information data and the third position information data, and processing at least one sample region of the sample corresponding to the at least one image region of the image defined in the aforementioned step d) with the laser microdissection system.