A radiograph analysis device configured to detect a marker area from a radiograph obtained by imaging a specimen in which a marker is embedded, the radiograph analysis device comprising: a brightness relation information acquisition unit configured to acquire brightness relation information, the brightness relation information generated based on information related to a quantity of radiation, the brightness relation information showing a relation between a brightness of the marker area and a brightness of a reference portion assumed to be a portion other than the marker; a reference brightness acquisition unit configured to acquire the brightness of the reference portion; and a marker area detection unit configured to detect the marker area based on the brightness relation information acquired by the brightness relation information acquisition unit and the brightness of the reference portion acquired by the reference brightness acquisition unit, characterized in that the brightness relation information acquisition unit is configured to acquire, as the brightness relation information, a function based on the brightness of the reference portion, the function being set based on a tube voltage, a tube current and an exposure time of a radiation source, the function showing a determination threshold of the brightness of the marker area, and the marker area detection unit is configured to detect a portion of the brightness equal to or smaller than a determination threshold as the marker area based on the determination threshold obtained by substituting the brightness of the reference portion acquired by the reference brightness acquisition unit for the function acquired by the brightness relation information acquisition unit. a brightness relation information acquisition unit configured to acquire brightness relation information, the brightness relation information generated based on information related to a quantity of radiation, the brightness relation information showing a relation between a brightness of the marker area and a brightness of a reference portion assumed to be a portion other than the marker; a reference brightness acquisition unit configured to acquire the brightness of the reference portion; and a marker area detection unit configured to detect the marker area based on the brightness relation information acquired by the brightness relation information acquisition unit and the brightness of the reference portion acquired by the reference brightness acquisition unit, characterized in that the brightness relation information acquisition unit is configured to acquire, as the brightness relation information, a function based on the brightness of the reference portion, the function being set based on a tube voltage, a tube current and an exposure time of a radiation source, the function showing a determination threshold of the brightness of the marker area, and the marker area detection unit is configured to detect a portion of the brightness equal to or smaller than a determination threshold as the marker area based on the determination threshold obtained by substituting the brightness of the reference portion acquired by the reference brightness acquisition unit for the function acquired by the brightness relation information acquisition unit.