Method for producing a 3D-printed tissue substitute, utilizing a 3D printing device comprising a print head equipped with at least one printing material delivery cartridge, said 3D printing device including a tank comprising a yield stress fluid, forming a gel in which said material is printed, said print head being mounted mobile in displacement, said printing material solidifying substantially under a first solidification temperature and said yield stress fluid solidifying substantially under a second solidification temperature, said method being characterized in that said printing material delivered by the cartridge includes polyvinyl alcohol and gelatin, in that said second solidification temperature of the yield stress fluid is lower than said first solidification temperature of said printing material, and in that said method includes the following steps: - solubilizing said printing material so as to obtain a sufficiently fluid material so that it is delivered by said cartridge, - supplying said 3D printing device with said solubilized material, - printing said material in said yield stress fluid in the tank by moving the print head into said tank and delivery of said material by said cartridge so as to form an intermediate device, - solidifying said intermediate device by placing said tank at a temperature lower than said first solidification temperature for a first time interval, said temperature however being higher than said second solidification temperature, then by placing the tank at ambient temperature for a second time interval, said ambient temperature being higher than said first and second solidification temperatures, - removing said solidified intermediate device from said tank, - rinsing said solidified intermediate device, and - drying said intermediate device in order to obtain said tissue substitute. in that said printing material delivered by the cartridge includes polyvinyl alcohol and gelatin, in that said second solidification temperature of the yield stress fluid is lower than said first solidification temperature of said printing material, and in that said method includes the following steps: - solubilizing said printing material so as to obtain a sufficiently fluid material so that it is delivered by said cartridge, - supplying said 3D printing device with said solubilized material, - printing said material in said yield stress fluid in the tank by moving the print head into said tank and delivery of said material by said cartridge so as to form an intermediate device, - solidifying said intermediate device by placing said tank at a temperature lower than said first solidification temperature for a first time interval, said temperature however being higher than said second solidification temperature, then by placing the tank at ambient temperature for a second time interval, said ambient temperature being higher than said first and second solidification temperatures, - removing said solidified intermediate device from said tank, - rinsing said solidified intermediate device, and - drying said intermediate device in order to obtain said tissue substitute. - solubilizing said printing material so as to obtain a sufficiently fluid material so that it is delivered by said cartridge, - supplying said 3D printing device with said solubilized material, - printing said material in said yield stress fluid in the tank by moving the print head into said tank and delivery of said material by said cartridge so as to form an intermediate device, - solidifying said intermediate device by placing said tank at a temperature lower than said first solidification temperature for a first time interval, said temperature however being higher than said second solidification temperature, then by placing the tank at ambient temperature for a second time interval, said ambient temperature being higher than said first and second solidification temperatures, - removing said solidified intermediate device from said tank, - rinsing said solidified intermediate device, and - drying said intermediate device in order to obtain said tissue substitute.