A system for performing fluorescent ligand scanning on a subject's eye, the system comprising: a light source configured to transmit light toward the subject's eye; a first microscope objective configured and disposed to focus light sent from the source toward the subject's eye to produce a focused spot of light to impinge the eye; an actuator coupled to a movable first lens and configured to position the focused spot of light sent from the first microscope objective through the movable first lens within the subject's eye; a lens configured to focus light sent from the source and emitted from the subject's eye; a detector configured and disposed to receive a first portion of the received light and configured to provide indicia of an image corresponding to the first portion of the received light; and a processor coupled to the detector and configured to analyze intensities of light in the image to determine a location of a reference point corresponding to an interface of a portion of the eye, characterized in that the system further comprises a memory comprising computer-readable, computer-executable instructions to control the processor to perform fluorescent ligand scanning by analyzing intensity of light emitted from a portion of the subject's eye, a location of the portion of the subject's eye being selected relative to the reference point, the analyzing of the intensity of light emitted from the portion of the subject's eye being to determine a physical property of material at the selected location based on fluorescence; and in that the memory further comprises computer-readable, computer-executable instructions to control the processor to correlate magnitudes of intensity of fluorescence from the eye with locations in the eye from which fluorescence was emitted, and to compare the magnitudes of intensity of fluorescence at the locations in the eye, after introduction of an imaging agent into the eye, with magnitudes of intensity of fluorescence emitted from similar locations before introducing the imaging agent into the eye. a light source configured to transmit light toward the subject's eye; a first microscope objective configured and disposed to focus light sent from the source toward the subject's eye to produce a focused spot of light to impinge the eye; an actuator coupled to a movable first lens and configured to position the focused spot of light sent from the first microscope objective through the movable first lens within the subject's eye; a lens configured to focus light sent from the source and emitted from the subject's eye; a detector configured and disposed to receive a first portion of the received light and configured to provide indicia of an image corresponding to the first portion of the received light; and a processor coupled to the detector and configured to analyze intensities of light in the image to determine a location of a reference point corresponding to an interface of a portion of the eye, characterized in that the system further comprises a memory comprising computer-readable, computer-executable instructions to control the processor to perform fluorescent ligand scanning by analyzing intensity of light emitted from a portion of the subject's eye, a location of the portion of the subject's eye being selected relative to the reference point, the analyzing of the intensity of light emitted from the portion of the subject's eye being to determine a physical property of material at the selected location based on fluorescence; and in that the memory further comprises computer-readable, computer-executable instructions to control the processor to correlate magnitudes of intensity of fluorescence from the eye with locations in the eye from which fluorescence was emitted, and to compare the magnitudes of intensity of fluorescence at the locations in the eye, after introduction of an imaging agent into the eye, with magnitudes of intensity of fluorescence emitted from similar locations before introducing the imaging agent into the eye.