A fluidic cartridge for carrying out a diagnostic test on a sample, the fluidic cartridge comprising: a front end comprising: a main channel fluidly connecting: a mixing chamber with a sample indicator and a blister; a coarse filter; a bellows having an upstream and a downstream bellows valve; two branch columns (26, 28) connecting a wash buffer blister upstream of a wash buffer valve and an elution buffer blister upstream of an elution buffer valve to the main channel; and a capture column; a waste branch containing a waste chamber valve fluidly connected to a waste chamber vented to atmosphere through an aerosol impactor and a waste vent; and an elution branch containing an elution chamber valve fluidly connected to an elution chamber and a mixing channel, a back end comprising: two amplification channels (54a-b), each amplification channel comprising an amplification chamber (56a-b) having an inlet valve (58a-b) and an outlet valve (60a-b); two bypass channels (66a-b) fluidly connecting the amplification channels to a bypass valve; and detection chambers (62a-d) downstream of the amplification chambers (56a-b) with gas springs (64a-d) downstream thereof to form a dead end, and an isolation valve comprising a valve chamber with a first opening fluidly connected to the front end and a second opening fluidly connected to the back end, and a flexible membrane overlying the valve chamber and moveable from an open position spaced apart from the openings to a closed position sealing at least one of the openings by an actuator. a front end comprising: a main channel fluidly connecting: a mixing chamber with a sample indicator and a blister; a coarse filter; a bellows having an upstream and a downstream bellows valve; two branch columns (26, 28) connecting a wash buffer blister upstream of a wash buffer valve and an elution buffer blister upstream of an elution buffer valve to the main channel; and a capture column; a waste branch containing a waste chamber valve fluidly connected to a waste chamber vented to atmosphere through an aerosol impactor and a waste vent; and an elution branch containing an elution chamber valve fluidly connected to an elution chamber and a mixing channel, a main channel fluidly connecting: a mixing chamber with a sample indicator and a blister; a coarse filter; a bellows having an upstream and a downstream bellows valve; two branch columns (26, 28) connecting a wash buffer blister upstream of a wash buffer valve and an elution buffer blister upstream of an elution buffer valve to the main channel; and a capture column; a waste branch containing a waste chamber valve fluidly connected to a waste chamber vented to atmosphere through an aerosol impactor and a waste vent; and an elution branch containing an elution chamber valve fluidly connected to an elution chamber and a mixing channel, a back end comprising: two amplification channels (54a-b), each amplification channel comprising an amplification chamber (56a-b) having an inlet valve (58a-b) and an outlet valve (60a-b); two bypass channels (66a-b) fluidly connecting the amplification channels to a bypass valve; and detection chambers (62a-d) downstream of the amplification chambers (56a-b) with gas springs (64a-d) downstream thereof to form a dead end, and two amplification channels (54a-b), each amplification channel comprising an amplification chamber (56a-b) having an inlet valve (58a-b) and an outlet valve (60a-b); two bypass channels (66a-b) fluidly connecting the amplification channels to a bypass valve; and detection chambers (62a-d) downstream of the amplification chambers (56a-b) with gas springs (64a-d) downstream thereof to form a dead end, and an isolation valve comprising a valve chamber with a first opening fluidly connected to the front end and a second opening fluidly connected to the back end, and a flexible membrane overlying the valve chamber and moveable from an open position spaced apart from the openings to a closed position sealing at least one of the openings by an actuator.