A system to authenticate packetized audio signals in voice-activated computer network environments, comprising: a data processing system comprising: one or more processors and memory; a natural language processor component configured to parse a first audio packet comprising a first input audio signal input via a sensor of a client device to determine that a service provider device is to be sent a request indicated in the first input audio signal; a conversational application programming interface configured to establish a communication session between the client device and the service provider device determined to be sent the request; a content selector component configured to select a content item to transmit to the client device, the content item comprising additional information to authenticate the first input audio signal, the content item including at least one of an output audio signal prompting for input audio or a visual notification prompting for an interaction, wherein the content item comprises a dynamically generated security question; and a network security appliance configured to: receive, from the client device, a second audio packet including a second input audio signal, the second input audio signal corresponding to a response to the content item provided to the client device; compare, for the authentication of the first audio packet, a characteristic of the second input audio signal of the second audio packet to a characteristic associated with the first input audio signal of the first audio packet; if the characteristic of the second input audio signal matches the characteristic associated with the first input audio signal, set a pass condition; and transmit, in accordance with the comparison for the authentication of the first audio packet, an instruction corresponding to a condition indicating one of a continuation or a termination of the communication session established between the service provider device and the client device. one or more processors and memory; a natural language processor component configured to parse a first audio packet comprising a first input audio signal input via a sensor of a client device to determine that a service provider device is to be sent a request indicated in the first input audio signal; a conversational application programming interface configured to establish a communication session between the client device and the service provider device determined to be sent the request; a content selector component configured to select a content item to transmit to the client device, the content item comprising additional information to authenticate the first input audio signal, the content item including at least one of an output audio signal prompting for input audio or a visual notification prompting for an interaction, wherein the content item comprises a dynamically generated security question; and a network security appliance configured to: receive, from the client device, a second audio packet including a second input audio signal, the second input audio signal corresponding to a response to the content item provided to the client device; compare, for the authentication of the first audio packet, a characteristic of the second input audio signal of the second audio packet to a characteristic associated with the first input audio signal of the first audio packet; if the characteristic of the second input audio signal matches the characteristic associated with the first input audio signal, set a pass condition; and transmit, in accordance with the comparison for the authentication of the first audio packet, an instruction corresponding to a condition indicating one of a continuation or a termination of the communication session established between the service provider device and the client device. receive, from the client device, a second audio packet including a second input audio signal, the second input audio signal corresponding to a response to the content item provided to the client device; compare, for the authentication of the first audio packet, a characteristic of the second input audio signal of the second audio packet to a characteristic associated with the first input audio signal of the first audio packet; if the characteristic of the second input audio signal matches the characteristic associated with the first input audio signal, set a pass condition; and transmit, in accordance with the comparison for the authentication of the first audio packet, an instruction corresponding to a condition indicating one of a continuation or a termination of the communication session established between the service provider device and the client device.