A microfluidic detection system for a refrigerator, comprising: a microfluidic biochip that has a sample inlet, a communication port and a detection pool formed therein, wherein the sample inlet, the detection pool and the communication port communicate in sequence by means of a microfluidic channel; a sample liquid driving device, which is in sealed communication with the communication port by means of a sealed docking mechanism, and is used to impel sample liquid in contact with the sample inlet to enter the microfluidic channel and flow to the detection pool by means of the microfluidic channel; and a detection mechanism, which is used for detecting the detection pool so as to obtain a preset detection parameter of the sample liquid, wherein the communication port of the microfluidic biochip is fixedly provided with a plug pin protruding and extending outward, an internal flow channel of the plug pin is in sealed communication with the communication port, and the plug pin is inserted into the inside of the sealed docking mechanism and forms a fluid-tight connection with the sealed docking mechanism, and the sealed docking mechanism is in fluid-tight connection with the sample liquid driving device, so that the sample liquid driving device is in sealed communication with the communication port; characterized in that an end surface of an extended tip of the plug pin is a continuous and smooth hemispherical surface, and a pin hole of the plug pin for fluid communication with the sealed docking mechanism is formed on the circumferential side of a section of the plug pin located inside the sealed docking mechanism. a microfluidic biochip that has a sample inlet, a communication port and a detection pool formed therein, wherein the sample inlet, the detection pool and the communication port communicate in sequence by means of a microfluidic channel; a sample liquid driving device, which is in sealed communication with the communication port by means of a sealed docking mechanism, and is used to impel sample liquid in contact with the sample inlet to enter the microfluidic channel and flow to the detection pool by means of the microfluidic channel; and a detection mechanism, which is used for detecting the detection pool so as to obtain a preset detection parameter of the sample liquid, wherein the communication port of the microfluidic biochip is fixedly provided with a plug pin protruding and extending outward, an internal flow channel of the plug pin is in sealed communication with the communication port, and the plug pin is inserted into the inside of the sealed docking mechanism and forms a fluid-tight connection with the sealed docking mechanism, and the sealed docking mechanism is in fluid-tight connection with the sample liquid driving device, so that the sample liquid driving device is in sealed communication with the communication port; characterized in that an end surface of an extended tip of the plug pin is a continuous and smooth hemispherical surface, and a pin hole of the plug pin for fluid communication with the sealed docking mechanism is formed on the circumferential side of a section of the plug pin located inside the sealed docking mechanism.