A pipette tip coupling device and pipette tip assembly comprising: a pipette tip comprising a circumscribing sidewall with an open interior passage including an interior surface and extending along a central longitudinal axis of the pipette tip between an open proximal end and an open distal end of the pipette tip; a pipette tip coupling device comprising: a shank; a coupler body; the shank has a proximal end face; the coupler body has a proximal coupler end surface; wherein the coupler body is distal to the shank, and wherein at least a portion of the coupler body has a diameter that is greater than a widest diameter of the shank; the pipette tip coupling device further comprising a distal stem portion; a distal end plate; an open-ended interior circumscribing surface forming an open passageway extending longitudinally from the proximal end face through the distal end plate; a proximal elastomeric element disposed around the shank and adjacent to the proximal coupler end surface; and a distal elastomeric element disposed around the distal stem portion and adjacent to the distal end plate; wherein the proximal elastomeric element abuts a first inner surface of the circumscribing sidewall of the pipette tip for securing the pipette tip to the pipette tip coupling device, and wherein the distal elastomeric element abuts a second inner surface of the circumscribing sidewall of the pipette tip to form a seal between the pipette tip coupling device and the pipette tip; characterized in that an annular wedge movably circumscribes the shank, the annular wedge comprising: a proximal wedge surface having a first wedge circumference; a distal wedge surface having a second wedge circumference less than the first wedge circumference; an exterior surface, wherein the exterior surface connects the proximal wedge surface with the distal wedge surface; and wherein the exterior surface of the annular wedge abuts the proximal elastomeric element. a pipette tip comprising a circumscribing sidewall with an open interior passage including an interior surface and extending along a central longitudinal axis of the pipette tip between an open proximal end and an open distal end of the pipette tip; a pipette tip coupling device comprising: a shank; a coupler body; the shank has a proximal end face; the coupler body has a proximal coupler end surface; wherein the coupler body is distal to the shank, and wherein at least a portion of the coupler body has a diameter that is greater than a widest diameter of the shank; the pipette tip coupling device further comprising a distal stem portion; a distal end plate; an open-ended interior circumscribing surface forming an open passageway extending longitudinally from the proximal end face through the distal end plate; a proximal elastomeric element disposed around the shank and adjacent to the proximal coupler end surface; and a distal elastomeric element disposed around the distal stem portion and adjacent to the distal end plate; a shank; a coupler body; the shank has a proximal end face; the coupler body has a proximal coupler end surface; wherein the coupler body is distal to the shank, and wherein at least a portion of the coupler body has a diameter that is greater than a widest diameter of the shank; the pipette tip coupling device further comprising a distal stem portion; a distal end plate; an open-ended interior circumscribing surface forming an open passageway extending longitudinally from the proximal end face through the distal end plate; a proximal elastomeric element disposed around the shank and adjacent to the proximal coupler end surface; and a distal elastomeric element disposed around the distal stem portion and adjacent to the distal end plate; wherein the proximal elastomeric element abuts a first inner surface of the circumscribing sidewall of the pipette tip for securing the pipette tip to the pipette tip coupling device, and wherein the distal elastomeric element abuts a second inner surface of the circumscribing sidewall of the pipette tip to form a seal between the pipette tip coupling device and the pipette tip; characterized in that an annular wedge movably circumscribes the shank, the annular wedge comprising: a proximal wedge surface having a first wedge circumference; a distal wedge surface having a second wedge circumference less than the first wedge circumference; an exterior surface, wherein the exterior surface connects the proximal wedge surface with the distal wedge surface; and wherein the exterior surface of the annular wedge abuts the proximal elastomeric element.