A fixed and permeabilized cell, comprising: (a) at least one target nucleic acid; (b) at least one set of two or more capture probes capable of hybridizing to said target nucleic acid; (c) a signal generating multimer capable of hybridizing to said set of two or more capture probes, wherein said signal generating multimer comprises a label probe; and (d) a signal amplification probe capable of binding to said label probe, wherein said signal amplification probe comprises: (i) a biotin molecule which is capable of conjugating to said label probe, an avidin/streptavidin molecule which is capable of binding to said biotin molecule, and additional biotin molecules which are conjugated to Horse Radish Peroxidase, Alkaline Phosphatase, or fluorophores and capable of binding to said avidin/streptavidin molecule; (ii) a HRP, AP, Dinitrophenyl, or fluorophore molecule which is capable of conjugating to said label probe, one or more first antibody which is capable of binding to said HRP, AP, DNP, or fluorophore molecule, and one or more second antibody which is conjugated to HRP, Polymer-HRP, AP, Polymer-AP, or fluorophore and capable of binding to said one or more first antibody; or (iii) a HRP molecule which is capable of conjugating to said label probe, a plurality of tyramide-fluorophore molecules or a plurality of tyramide-biotin or tyramide-hapten molecules which are capable of reacting with said HRP molecule, and detection labels which are capable of detecting said tyramide-biotin or tyramide-hapten molecules visually, wherein said set of two or more capture probes is hybridized to said target nucleic acid in the cell; said signal generating multimer is captured to said set of two or more capture probes; and said signal amplification probe is captured to said label probe of said signal generating multimer. (a) at least one target nucleic acid; (b) at least one set of two or more capture probes capable of hybridizing to said target nucleic acid; (c) a signal generating multimer capable of hybridizing to said set of two or more capture probes, wherein said signal generating multimer comprises a label probe; and (d) a signal amplification probe capable of binding to said label probe, wherein said signal amplification probe comprises: (i) a biotin molecule which is capable of conjugating to said label probe, an avidin/streptavidin molecule which is capable of binding to said biotin molecule, and additional biotin molecules which are conjugated to Horse Radish Peroxidase, Alkaline Phosphatase, or fluorophores and capable of binding to said avidin/streptavidin molecule; (ii) a HRP, AP, Dinitrophenyl, or fluorophore molecule which is capable of conjugating to said label probe, one or more first antibody which is capable of binding to said HRP, AP, DNP, or fluorophore molecule, and one or more second antibody which is conjugated to HRP, Polymer-HRP, AP, Polymer-AP, or fluorophore and capable of binding to said one or more first antibody; or (iii) a HRP molecule which is capable of conjugating to said label probe, a plurality of tyramide-fluorophore molecules or a plurality of tyramide-biotin or tyramide-hapten molecules which are capable of reacting with said HRP molecule, and detection labels which are capable of detecting said tyramide-biotin or tyramide-hapten molecules visually, (i) a biotin molecule which is capable of conjugating to said label probe, an avidin/streptavidin molecule which is capable of binding to said biotin molecule, and additional biotin molecules which are conjugated to Horse Radish Peroxidase, Alkaline Phosphatase, or fluorophores and capable of binding to said avidin/streptavidin molecule; (ii) a HRP, AP, Dinitrophenyl, or fluorophore molecule which is capable of conjugating to said label probe, one or more first antibody which is capable of binding to said HRP, AP, DNP, or fluorophore molecule, and one or more second antibody which is conjugated to HRP, Polymer-HRP, AP, Polymer-AP, or fluorophore and capable of binding to said one or more first antibody; or (iii) a HRP molecule which is capable of conjugating to said label probe, a plurality of tyramide-fluorophore molecules or a plurality of tyramide-biotin or tyramide-hapten molecules which are capable of reacting with said HRP molecule, and detection labels which are capable of detecting said tyramide-biotin or tyramide-hapten molecules visually,