mhurhangee/ep-ind-claims huggingface dataset:
A method of creating a library of nucleotides encoding human immunoglobulin V- regions, the method comprising the steps of: (a) using a first PCR to amplify human immunoglobulin heavy and light chain nucleotide sequences from a cDNA library made from a sample of human peripheral blood mononuclear cells obtained from a human donor previously determined to possess antibodies specific for target antigens of interest, wherein leader-specific forward primers that amplify at least 90% of the human immunoglobulin V-region genes and reverse primers from a sequence located 3' of the nucleotides encoding the V-region, such as nucleic acid sequences encoding hinge or constant regions are used in the first PCR amplification and the leader-specific primers comprise at least 50 different primers, wherein each of the different primers comprises a different nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-76; (b) isolating the amplification products from the first PCR step; (c) creating a first set of constructs by inserting the isolated amplification products from the first PCR step into expression vectors; and (d) introducing the first set of constructs into a first set of host cells to create a first library of human immunoglobulin nucleotide sequences. (a) using a first PCR to amplify human immunoglobulin heavy and light chain nucleotide sequences from a cDNA library made from a sample of human peripheral blood mononuclear cells obtained from a human donor previously determined to possess antibodies specific for target antigens of interest, wherein leader-specific forward primers that amplify at least 90% of the human immunoglobulin V-region genes and reverse primers from a sequence located 3' of the nucleotides encoding the V-region, such as nucleic acid sequences encoding hinge or constant regions are used in the first PCR amplification and the leader-specific primers comprise at least 50 different primers, wherein each of the different primers comprises a different nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-76; (b) isolating the amplification products from the first PCR step; (c) creating a first set of constructs by inserting the isolated amplification products from the first PCR step into expression vectors; and (d) introducing the first set of constructs into a first set of host cells to create a first library of human immunoglobulin nucleotide sequences.
An assay cartridge for analyzing a sample collected with an applicator stick comprising a shaft and a sample collection head, said cartridge comprising a sample chamber (3010, 3220) having a bend or angle along its length such that the sample chamber (3010, 3220) has a first elongated region on one side of the bend or angle oriented in one direction and a second elongated region on the other side of the bend or angle oriented in a second direction, said regions being oriented at an angle of 30 to 70 degrees with respect to each other, wherein said angle at which said regions are oriented with respect to each other is selected to bend said shaft upon insertion of said applicator stick into said sample chamber (3010, 3220) and promote fracture of said shaft. said cartridge comprising a sample chamber (3010, 3220) having a bend or angle along its length such that the sample chamber (3010, 3220) has a first elongated region on one side of the bend or angle oriented in one direction and a second elongated region on the other side of the bend or angle oriented in a second direction, said regions being oriented at an angle of 30 to 70 degrees with respect to each other, wherein said angle at which said regions are oriented with respect to each other is selected to bend said shaft upon insertion of said applicator stick into said sample chamber (3010, 3220) and promote fracture of said shaft.
A cloaking system, comprising: a structure having a plurality of resonators having a controllable resonance frequency, wherein said resonators are arranged to collectively ensure that variation of said resonance frequency over a predetermined range of resonance frequencies generates a phase shift between an electromagnetic wave incident on said structure and an electromagnetic wave scattered off said structure; and a controller configured for controlling said resonance frequency to provide a time-varying resonance frequency having a temporal function which comprises a linear time-dependence; characterized in that said controller is configured to receive velocity data characterizing a motion of a vehicle and to select said time-varying resonance frequency based on said velocity data. a structure having a plurality of resonators having a controllable resonance frequency, wherein said resonators are arranged to collectively ensure that variation of said resonance frequency over a predetermined range of resonance frequencies generates a phase shift between an electromagnetic wave incident on said structure and an electromagnetic wave scattered off said structure; and a controller configured for controlling said resonance frequency to provide a time-varying resonance frequency having a temporal function which comprises a linear time-dependence; characterized in that said controller is configured to receive velocity data characterizing a motion of a vehicle and to select said time-varying resonance frequency based on said velocity data.
A method of fabricating resin infused composite parts, comprising: placing a perforated caul sheet over a component to be infused with resin, the component having first component portions having a first thickness and second component portions having a second thickness, the first thickness greater than the second thickness, the caul sheet having first caul areas having a first density of perforations and second caul areas having a second density of perforations, the first density of perforations being higher than the second density of perforations, and wherein the first caul areas overlie the first component portions and the second caul areas overlie the second component portions; and infusing the component with resin, including controlling the flow of resin into the component using the caul sheet. placing a perforated caul sheet over a component to be infused with resin, the component having first component portions having a first thickness and second component portions having a second thickness, the first thickness greater than the second thickness, the caul sheet having first caul areas having a first density of perforations and second caul areas having a second density of perforations, the first density of perforations being higher than the second density of perforations, and wherein the first caul areas overlie the first component portions and the second caul areas overlie the second component portions; and infusing the component with resin, including controlling the flow of resin into the component using the caul sheet.
Device for sterilising containers and in particular plastics material preforms, with a sterilisation chamber and a transport device disposed at least partially inside the sterilisation chamber for moving the plastics material preforms within the sterilisation chamber, wherein the transport device has at least one gripping system for engaging round and supporting the plastics material preforms by means of a gripping clamp, wherein the gripping system has a displacement means for displacement during sterilisation within the sterilisation chamber of contact surfaces on an external surface of the plastics material preforms which are shielded by the gripping system from a sterilising medium and/or sterilising radiation, characterized in that the displacement means is formed with at least one further gripping clamp, both gripping clamps (41, 42) being arranged in a vertical direction at least partly in an overlapping manner and at a distance with respect to each other in this direction and wherein during sterilization at least one gripping clamp is able to be operated in an open position and a gripping clamp different thereto is able to be operated in a closed position and after a predefined partial sterilization time the respective different gripping clamp is able to be operated in an open or in a closed position.
Composition consisting essentially of (i) a protein; (ii) a taxane or taxoid; and (iii) a pharmaceutically acceptable diluent, wherein the taxane or taxoid is bonded with the protein via a direct bond between the taxane or taxoid and the protein; wherein the taxane or taxoid is paclitaxel or docetaxel; wherein the protein is a metal-transferrin; and wherein the metal-transferrin is gallium-transferrin, iron-transferrin, indium-transferrin, zinc-transferrin, manganese-transferrin, platinum-transferrin, or a mixture thereof.
A portable container for one or more articles each tagged with a respective Radio Frequency Identification (RFID) device, wherein the container comprises: a bay having at least one opening for removal and/or placement of RFID-tagged articles in the bay; a lid for covering the at least one opening in the bay, wherein the lid is openable to permit access to the bay; a detector device (21,22) for detecting RFID-tagged articles located in the bay; and a power supply for supplying electrical power to the detector device (21,22); characterised in that the container comprises: an electromagnetic shield wherein the bay and the at least one opening in the bay is enclosed by the electromagnetic shield and wherein the lid forms part of the electromagnetic shield. a bay having at least one opening for removal and/or placement of RFID-tagged articles in the bay; a lid for covering the at least one opening in the bay, wherein the lid is openable to permit access to the bay; a detector device (21,22) for detecting RFID-tagged articles located in the bay; and a power supply for supplying electrical power to the detector device (21,22); an electromagnetic shield wherein the bay and the at least one opening in the bay is enclosed by the electromagnetic shield and wherein the lid forms part of the electromagnetic shield.
Bellows-type air spring (1, 15, 21) having two or more bellows sections designed as folds or toroidal parts (2, 3), which are separated by rings placed around the air spring bellows, more particularly metal rings, wherein the bellows-type air spring is secured between the sprung and the unsprung mass with the aid of connecting parts (6, 7, 18, 24, 25, 26) and wherein the bellows (17, 23) project outward in the manner of a balloon between the connecting parts, or the folds or toroidal parts (2, 3) of the bellows project outward in the manner of a balloon between or adjacent to the rings, characterized in that annular sliding elements (8, 9, 12, 13, 16, 20, 22), more particularly sliding elements designed as sliding discs, are arranged between adjacent bellows sections and/or between bellows sections and connecting sections, against which sliding elements the bellows sections or folds or toroidal parts come to rest when a load is applied to the folding-bellows-type air spring, wherein in each case at least two annular sliding discs (8, 9, 12, 13) arranged so as to slide on one another, the inside diameter of which is greater than the outside diameter of the rings separating the folds or toroidal parts, are arranged between adjacent folds or toroidal parts.
A method for laser beam machining of a workpiece, wherein a laser beam is focused by an objective having a focal plane into or onto the workpiece, which has a boundary surface, to produce a machining effect by two-photon processes, and the position of the focus with respect to the workpiece is shifted, characterized in that to reference the position of the focus, an illuminating modulation object is projected onto the workpiece, likewise by the objective, in the focal plane or so as to intersect the focal plane, reflections of the image occurring at the boundary surface are projected into an autofocus image plane, and are detected by a camera having a camera image plane, wherein the camera image plane either intersects the autofocus image plane when the image of the illuminating modulation object lies in the focal plane, or lies in the autofocus image plane when the image of the modulation object intersects the focal plane.
A power converter configured to mutually convert power between AC and DC the power converter comprising: first, second, third, and fourth switching elements (11,12,13,14) having self-extinguishing properties and configured to be connected in series to a DC power supply in an ascending order, a collector of the first switching element being connected to a DC positive terminal of the DC power supply, an emitter of the fourth switching element being connected to a DC negative terminal of the DC power supply; first and second chopper cell group circuits (15, 16) each comprising one or more chopper cells (ch_up1,..., ch_un1,...) connected in series that comprise a leg having two self-extinguishing type switching elements (sw_ch1, sw_ch2) connected in series, and a capacitor (c_ch) connected to the leg in parallel therewith; and wherein: the second switching element has a collector connected to a positive terminal of the first chopper cell group circuit the third switching element has an emitter connected to a negative terminal of the second chopper cell group circuit; and the first chopper cell group circuit has a negative terminal connected to a positive terminal of the second chopper cell, characterized by first and second capacitors (18, 19) connected with each other in series and connected in parallel with the DC power supply, a DC neutral point divided by the first and second capacitors, a reactor inserted in a path from a connection node between the second switching element and the third switching element to the DC neutral point, wherein a connection node between the first and second chopper cell group circuits (15, 16) is configured to serve as an output AC terminal. first, second, third, and fourth switching elements (11,12,13,14) having self-extinguishing properties and configured to be connected in series to a DC power supply in an ascending order, a collector of the first switching element being connected to a DC positive terminal of the DC power supply, an emitter of the fourth switching element being connected to a DC negative terminal of the DC power supply; first and second chopper cell group circuits (15, 16) each comprising one or more chopper cells (ch_up1,..., ch_un1,...) connected in series that comprise a leg having two self-extinguishing type switching elements (sw_ch1, sw_ch2) connected in series, and a capacitor (c_ch) connected to the leg in parallel therewith; and wherein: the second switching element has a collector connected to a positive terminal of the first chopper cell group circuit the third switching element has an emitter connected to a negative terminal of the second chopper cell group circuit; and the first chopper cell group circuit has a negative terminal connected to a positive terminal of the second chopper cell, the second switching element has a collector connected to a positive terminal of the first chopper cell group circuit the third switching element has an emitter connected to a negative terminal of the second chopper cell group circuit; and the first chopper cell group circuit has a negative terminal connected to a positive terminal of the second chopper cell,
A method for producing potassium titanate, the method comprising the steps of: 1) preparing a mixed solution including a titanium precursor and a potassium precursor; and 2) heat-treating the mixed solution to synthesize potassium titanate, wherein the titanium precursor is anatase-phased titanium dioxide a-TiO 2, the heat treatment temperature is 200 to 300°C, and the heat treatment time is 3 hours or more, characterized in that the molar ratio of the titanium precursor and the potassium precursor is 1:500 to 1:600. 1) preparing a mixed solution including a titanium precursor and a potassium precursor; and 2) heat-treating the mixed solution to synthesize potassium titanate, wherein the titanium precursor is anatase-phased titanium dioxide a-TiO 2, the heat treatment temperature is 200 to 300°C, and the heat treatment time is 3 hours or more, characterized in that the molar ratio of the titanium precursor and the potassium precursor is 1:500 to 1:600.
An in vitro method for producing a population of induced pluripotent stem (iPS) cells, comprising: a) introducing into somatic cells a first nucleic acid molecule for integration into the genome of the cells and at least a second nucleic acid molecule comprising an extra-chromosomal genetic element that expresses one or more reprogramming factors sufficient when expressed in the somatic cell to convert the somatic cell to a pluripotent stem cell; b) culturing said cells under reprogramming conditions; and c) obtaining a population of iPS cells comprising said first nucleic acid integrated in their genome, wherein said first nucleic acid is expressible and wherein the second nucleic acid molecule is not present in the iPS cells. a) introducing into somatic cells a first nucleic acid molecule for integration into the genome of the cells and at least a second nucleic acid molecule comprising an extra-chromosomal genetic element that expresses one or more reprogramming factors sufficient when expressed in the somatic cell to convert the somatic cell to a pluripotent stem cell; b) culturing said cells under reprogramming conditions; and c) obtaining a population of iPS cells comprising said first nucleic acid integrated in their genome, wherein said first nucleic acid is expressible and wherein the second nucleic acid molecule is not present in the iPS cells.
An electron beam irradiation apparatus, comprising: a vacuum room including a wall having an opening for irradiating an electron beam, an internal atmosphere of said vacuum room being evacuated; an electron beam generator that is provided inside said vacuum room; a window frame that is attached to and surrounds said opening of said vacuum room; an irradiation foil fixed in said window frame through which an electron beam generated in said vacuum room is transmitted; and a surface of said window frame, at least an area exposed to said vacuum room, being substantially covered with material including an element or elements with an atomic number less than or equal to 10, characterized in that said material comprises carbon-fiber-reinforced plastic. a vacuum room including a wall having an opening for irradiating an electron beam, an internal atmosphere of said vacuum room being evacuated; an electron beam generator that is provided inside said vacuum room; a window frame that is attached to and surrounds said opening of said vacuum room; an irradiation foil fixed in said window frame through which an electron beam generated in said vacuum room is transmitted; and a surface of said window frame, at least an area exposed to said vacuum room, being substantially covered with material including an element or elements with an atomic number less than or equal to 10, characterized in that said material comprises carbon-fiber-reinforced plastic.
Method of applying coatings to surfaces, wherein a gas flow forms a gas-powder mixture with a powder of a material selected from the group consisting of a metal selected from niobium, tantalum, tungsten, molybdenum, titanium, zirconium, mixture of two or more thereof, alloys of two or more thereof and alloys thereof with other metals, the powder has a particle size of from 0.5 to 150 μm, wherein a supersonic speed is imparted to the gas flow and the jet of supersonic speed is directed onto the surface of an object, wherein the metal powder has an oxygen content of less than 1000 ppm oxygen and wherein the spraying comprises the steps of: - providing a spraying orifice adjacent a surface to be coated by spraying; - providing to the spraying orifice a powder of a particulate material selected from the group consisting of a metal selected from niobium, tantalum, tungsten, molybdenum, titanium, zirconium, mixture of two or more thereof, alloys of two or more thereof and alloys thereof with other metals, the powder having a particle size of 0.5 to 150 μm, said powder being under pressure; - providing an inert gas under pressure to the spraying orifice to establish a static pressure at the spraying orifice and providing a spray of said particulate material and gas onto the surface to be coated; and - locating the spraying orifice in a region of low ambient pressure which is less than 1 atmosphere and which is substantially less than the static pressure at the spraying orifice to provide substantial acceleration of the spray of said particulate material and gas onto said surface to be coated. - providing a spraying orifice adjacent a surface to be coated by spraying; - providing to the spraying orifice a powder of a particulate material selected from the group consisting of a metal selected from niobium, tantalum, tungsten, molybdenum, titanium, zirconium, mixture of two or more thereof, alloys of two or more thereof and alloys thereof with other metals, the powder having a particle size of 0.5 to 150 μm, said powder being under pressure; - providing an inert gas under pressure to the spraying orifice to establish a static pressure at the spraying orifice and providing a spray of said particulate material and gas onto the surface to be coated; and - locating the spraying orifice in a region of low ambient pressure which is less than 1 atmosphere and which is substantially less than the static pressure at the spraying orifice to provide substantial acceleration of the spray of said particulate material and gas onto said surface to be coated.
Assembly, comprising a generator and electric motors, for a vehicle air-conditioning or cooling system, comprising: - a compressor intended to be driven by a heat engine, - a top-up electric motor for driving the compressor when the latter is not or cannot be driven by the heat engine, the top-up motor comprising a squirrel cage rotor comprising a stack of magnetic plates comprising notches, the notches receiving aluminium or copper injected under pressure, - a generator with permanent magnets having a shaft which can be driven in rotation by the heat engine, the generator and the top-up electric motor being disposed on this shaft, - one or more electric motors of condensers and/or of evaporators (8, 9) of a vehicle air-conditioning or cooling system, said electric motors (8, 9) being configured to be powered by a direct current, the generator powering the electric motor or motors (8, 9) through a converter configured to supply a direct current, notably a low-voltage controller, the condenser and/or evaporator electric motors being variable-speed motors. - a compressor intended to be driven by a heat engine, - a top-up electric motor for driving the compressor when the latter is not or cannot be driven by the heat engine, the top-up motor comprising a squirrel cage rotor comprising a stack of magnetic plates comprising notches, the notches receiving aluminium or copper injected under pressure, - a generator with permanent magnets having a shaft which can be driven in rotation by the heat engine, the generator and the top-up electric motor being disposed on this shaft, - one or more electric motors of condensers and/or of evaporators (8, 9) of a vehicle air-conditioning or cooling system, said electric motors (8, 9) being configured to be powered by a direct current, the generator powering the electric motor or motors (8, 9) through a converter configured to supply a direct current, notably a low-voltage controller,