A guiding assembly for spinal drilling operation, which is disposed across a spinous process of vertebra, and contacts against a surface of lamina of the vertebra, comprising: a guiding element configured for disposing on the vertebra, wherein the guiding element comprises: a main body comprising two stand portions, two connection parts, two locating parts connecting to said two stand portions of the main body through the connection parts, respectively; and two fixing portions; characterized in that the guiding assembly further comprising: an auxiliary element mounted on one of the locating parts; a k-pin mounted on the auxiliary element; and a cannular driller mounted on the k-pin and having a holding part and a drilling part, wherein the main body further comprises two contact portions and a hand-held portion, wherein the contact portions are connected with the stand portions (311 a), respectively, the contact portions are disposed along the vertebra, the stand portions extends along a first direction, the contact portion is protruded from the stand portion along a second direction, and the first direction is different from the second direction, the fixing portions are connected to the contact portions, respectively, and the fixing portions (311 c) attach to the laminas of the vertebra; wherein each of the fixing portions has a hook shape, wherein the stand portions are configured to stand across the spinous process of the vertebra, and hand-held portion is connected to the stand portions (311 a), wherein the connection parts are direct physically connected to the stand portions, respectively, and the connection parts are connected to the contact portion through the stand portions, respectively. a guiding element configured for disposing on the vertebra, wherein the guiding element comprises: a main body comprising two stand portions, two connection parts, two locating parts connecting to said two stand portions of the main body through the connection parts, respectively; and a main body comprising two stand portions, two connection parts, two locating parts connecting to said two stand portions of the main body through the connection parts, respectively; and two fixing portions; an auxiliary element mounted on one of the locating parts; a k-pin mounted on the auxiliary element; and a cannular driller mounted on the k-pin and having a holding part and a drilling part, wherein the main body further comprises two contact portions and a hand-held portion, wherein the contact portions are connected with the stand portions (311 a), respectively, the contact portions are disposed along the vertebra, the stand portions extends along a first direction, the contact portion is protruded from the stand portion along a second direction, and the first direction is different from the second direction, the fixing portions are connected to the contact portions, respectively, and the fixing portions (311 c) attach to the laminas of the vertebra; wherein each of the fixing portions has a hook shape, wherein the stand portions are configured to stand across the spinous process of the vertebra, and hand-held portion is connected to the stand portions (311 a), wherein the connection parts are direct physically connected to the stand portions, respectively, and the connection parts are connected to the contact portion through the stand portions, respectively.