A method comprising: providing double-stranded nucleic acid material comprising one or more double-stranded nucleic acid molecules, wherein each double-stranded nucleic acid molecule comprises a single molecule identifier sequence on each strand and an adapter on at least one of the 5' and/or 3' ends of the nucleic acid molecule, and wherein, for each nucleic acid molecule, a first adapter sequence is associated with a first strand and a second adapter sequence is associated with a second strand of the nucleic acid molecule and wherein the adapter comprises at least one nucleotide position that is at least partially non-complimentary; amplifying the nucleic acid material; separating the amplified nucleic acid material into a first sample and a second sample; amplifying the first strand in the first sample using at least one single-stranded oligonucleotide at least partially complementary to a sequence in the first adapter sequence and at least one single-stranded oligonucleotide at least partially complementary to a target sequence of interest such that the single molecule identifier sequence is at least partially maintained to provide a first nucleic acid product; amplifying the second strand in the second sample using at least one single-stranded oligonucleotide at least partially complementary to a sequence present in the second adapter sequence and at least one single-stranded oligonucleotide at least partially complementary to the target sequence of interest such that the single molecule identifier sequence is at least partially maintained to provide a second nucleic acid product; sequencing each of the first nucleic acid product and second nucleic acid product; and comparing the sequence of the first nucleic acid product to the sequence of the second nucleic acid product. providing double-stranded nucleic acid material comprising one or more double-stranded nucleic acid molecules, wherein each double-stranded nucleic acid molecule comprises a single molecule identifier sequence on each strand and an adapter on at least one of the 5' and/or 3' ends of the nucleic acid molecule, and wherein, for each nucleic acid molecule, a first adapter sequence is associated with a first strand and a second adapter sequence is associated with a second strand of the nucleic acid molecule and wherein the adapter comprises at least one nucleotide position that is at least partially non-complimentary; amplifying the nucleic acid material; separating the amplified nucleic acid material into a first sample and a second sample; amplifying the first strand in the first sample using at least one single-stranded oligonucleotide at least partially complementary to a sequence in the first adapter sequence and at least one single-stranded oligonucleotide at least partially complementary to a target sequence of interest such that the single molecule identifier sequence is at least partially maintained to provide a first nucleic acid product; amplifying the second strand in the second sample using at least one single-stranded oligonucleotide at least partially complementary to a sequence present in the second adapter sequence and at least one single-stranded oligonucleotide at least partially complementary to the target sequence of interest such that the single molecule identifier sequence is at least partially maintained to provide a second nucleic acid product; sequencing each of the first nucleic acid product and second nucleic acid product; and comparing the sequence of the first nucleic acid product to the sequence of the second nucleic acid product.