A composition comprising: (i) a polynucleotide that comprises an open reading frame encoding a Jagged1 polypeptide and (ii) a delivery agent, wherein the delivery agent comprises a compound that is of Formula: or a salt or isomer thereof, wherein l is selected from 1, 2, 3, 4, and 5; m is selected from 5, 6, 7, 8, and 9 M 1 is a bond or M'; R 4 is unsubstituted C 1-3 alkyl, or -(CH 2) n Q, in which n is 1, 2, 3, 4 or 5 and Q is OH, -NHC N 2, -NHC N 2, -N C R, -N S 2 R, -N R 8, -NHC(=NR 9)N 2, -NHC(=CHR 9)N 2, -OC N 2, -N C OR, heteroaryl or heterocycloalkyl; M and M' are independently selected from - C O, OC, C N(R'), P (OR')O, -S-S-, an aryl group, and a heteroaryl group; R 2 and R 3 are independently selected from the group consisting of H, C 1-14 alkyl, and C 2-14 alkenyl; R is independently selected from the group consisting of C 1-3 alkyl, C 2-3 alkenyl, and H; R' is independently selected from the group consisting of C 1-18 alkyl, C 2-18 alkenyl, -R*YR", -YR", and H; R" is independently selected from the group consisting of C 3-14 alkyl and C 3-14 alkenyl; each R* is independently selected from the group consisting of C 1-12 alkyl and C 2-12 alkenyl; and each Y is independently a C 3-6 carbocycle. (i) a polynucleotide that comprises an open reading frame encoding a Jagged1 polypeptide and (ii) a delivery agent, wherein the delivery agent comprises a compound that is of Formula: or a salt or isomer thereof, wherein l is selected from 1, 2, 3, 4, and 5; m is selected from 5, 6, 7, 8, and 9 M 1 is a bond or M'; R 4 is unsubstituted C 1-3 alkyl, or -(CH 2) n Q, in which n is 1, 2, 3, 4 or 5 and Q is OH, -NHC N 2, -NHC N 2, -N C R, -N S 2 R, -N R 8, -NHC(=NR 9)N 2, -NHC(=CHR 9)N 2, -OC N 2, -N C OR, heteroaryl or heterocycloalkyl; M and M' are independently selected from - C O, OC, C N(R'), P (OR')O, -S-S-, an aryl group, and a heteroaryl group; R 2 and R 3 are independently selected from the group consisting of H, C 1-14 alkyl, and C 2-14 alkenyl; R is independently selected from the group consisting of C 1-3 alkyl, C 2-3 alkenyl, and H; R' is independently selected from the group consisting of C 1-18 alkyl, C 2-18 alkenyl, -R*YR", -YR", and H; R" is independently selected from the group consisting of C 3-14 alkyl and C 3-14 alkenyl; each R* is independently selected from the group consisting of C 1-12 alkyl and C 2-12 alkenyl; and each Y is independently a C 3-6 carbocycle. l is selected from 1, 2, 3, 4, and 5; m is selected from 5, 6, 7, 8, and 9 M 1 is a bond or M'; R 4 is unsubstituted C 1-3 alkyl, or -(CH 2) n Q, in which n is 1, 2, 3, 4 or 5 and Q is OH, -NHC N 2, -NHC N 2, -N C R, -N S 2 R, -N R 8, -NHC(=NR 9)N 2, -NHC(=CHR 9)N 2, -OC N 2, -N C OR, heteroaryl or heterocycloalkyl; M and M' are independently selected from - C O, OC, C N(R'), P (OR')O, -S-S-, an aryl group, and a heteroaryl group; R 2 and R 3 are independently selected from the group consisting of H, C 1-14 alkyl, and C 2-14 alkenyl; R is independently selected from the group consisting of C 1-3 alkyl, C 2-3 alkenyl, and H; R' is independently selected from the group consisting of C 1-18 alkyl, C 2-18 alkenyl, -R*YR", -YR", and H; R" is independently selected from the group consisting of C 3-14 alkyl and C 3-14 alkenyl; each R* is independently selected from the group consisting of C 1-12 alkyl and C 2-12 alkenyl; and each Y is independently a C 3-6 carbocycle.