An OLED array substrate comprising a first electrode layer (202, 302), a pixel definition layer (204, 304), an organic material functional layer (203, 303), and a second electrode layer arranged successively in a light exit direction, wherein the organic material functional layer (203, 303) comprises light-emitting regions being configured to emit light of different colors, the pixel definition layer (204, 304) comprises pixel regions corresponding to the light-emitting regions and spacing regions arranged between adjacent pixel regions, and each spacing region is configured to electrically isolate adjacent light-emitting regions, wherein the organic material functional layer comprises a first functional layer (2031, 3031), a light-emitting layer (2032, 3032), and a second functional layer (2033, 3033) arranged successively in the light exit direction, and each spacing region comprises a first spacing portion (2041, 3041) and a second spacing portion (2042, 3042) arranged successively in the light exit direction, wherein a thickness of the first spacing portion (2041, 3041) is greater than a thickness of the first functional layer (2031, 3031) so that portions of the first functional layer (2031, 3031) corresponding to respective light-emitting regions are separated from each other, characterized in that, a sum of a thickness of the second spacing portion (2042, 3042) and the thickness of the first spacing portion (2041, 3041) is greater than a sum of the thickness of the first functional layer (2031,3031) and a thickness of the light-emitting layer (2032, 3032) and smaller than a sum of the first functional layer (2031, 3031), the thickness of the light-emitting layer (2032, 3032) and a thickness of the second functional layer (2033, 3033), so that a plurality of second functional (2033, 3033) comprising the second functional layer (2033, 3033) and corresponding to respective light-emitting regions are connected with each other and continuous, whereas the first functional layer (2031, 3031) and the light-emitting layer (2032, 3032) of one light-emitting region are disconnected from the first functional layer (2031, 3031) and the light-emitting layer (2032, 3032) of any adjacent light-emitting region. the organic material functional layer (203, 303) comprises light-emitting regions being configured to emit light of different colors, the pixel definition layer (204, 304) comprises pixel regions corresponding to the light-emitting regions and spacing regions arranged between adjacent pixel regions, and each spacing region is configured to electrically isolate adjacent light-emitting regions, wherein the organic material functional layer comprises a first functional layer (2031, 3031), a light-emitting layer (2032, 3032), and a second functional layer (2033, 3033) arranged successively in the light exit direction, and each spacing region comprises a first spacing portion (2041, 3041) and a second spacing portion (2042, 3042) arranged successively in the light exit direction, wherein a thickness of the first spacing portion (2041, 3041) is greater than a thickness of the first functional layer (2031, 3031) so that portions of the first functional layer (2031, 3031) corresponding to respective light-emitting regions are separated from each other, characterized in that, a sum of a thickness of the second spacing portion (2042, 3042) and the thickness of the first spacing portion (2041, 3041) is greater than a sum of the thickness of the first functional layer (2031,3031) and a thickness of the light-emitting layer (2032, 3032) and smaller than a sum of the first functional layer (2031, 3031), the thickness of the light-emitting layer (2032, 3032) and a thickness of the second functional layer (2033, 3033), so that a plurality of second functional (2033, 3033) comprising the second functional layer (2033, 3033) and corresponding to respective light-emitting regions are connected with each other and continuous, whereas the first functional layer (2031, 3031) and the light-emitting layer (2032, 3032) of one light-emitting region are disconnected from the first functional layer (2031, 3031) and the light-emitting layer (2032, 3032) of any adjacent light-emitting region.