mhurhangee/ep-ind-claims huggingface dataset:
A method for the isolation and identification of at least one stably transduced vertebrate host cell that recombinantly expresses on its cell surface an antibody or fragment thereof specific for a desired antigen or ligand, comprising the steps of: (a) transducing at least one retroviral expression construct encoding an antibody or fragment thereof into vertebrate host cells by using replication incompetent retroviral particles, wherein the at least one construct stably integrates into the host cell genome such that the transduced host cells are capable of expressing and displaying said antibody or fragment thereof on their cell surface, and wherein the vertebrate host cells are precursor B lymphocytes that endogenously express Igα and Igβ molecules facilitating membrane deposition of said antibody or fragment thereof, and are unable to express endogenous antibody polypeptides and at least one surrogate light chain component; (b) stably expressing said antibody or fragment thereof in said vertebrate host cells and displaying the same on their cell surface; (c) enriching said vertebrate host cells expressing said antibody or fragment thereof on the basis of their ability to bind to said desired antigen or ligand by separating cells that exhibit specific antigen or ligand binding from a non-binding cell population, thus selectively isolating strong antibody binders having high affinity to said desired antigen or ligand; and (d) isolating and identifying said at least one stably transduced vertebrate host cell expressing said antibody or fragment thereof specific for the desired antigen or ligand on its cell surface from the retrovirally transduced and enriched vertebrate host cells. (a) transducing at least one retroviral expression construct encoding an antibody or fragment thereof into vertebrate host cells by using replication incompetent retroviral particles, wherein the at least one construct stably integrates into the host cell genome such that the transduced host cells are capable of expressing and displaying said antibody or fragment thereof on their cell surface, and wherein the vertebrate host cells are precursor B lymphocytes that endogenously express Igα and Igβ molecules facilitating membrane deposition of said antibody or fragment thereof, and are unable to express endogenous antibody polypeptides and at least one surrogate light chain component; (b) stably expressing said antibody or fragment thereof in said vertebrate host cells and displaying the same on their cell surface; (c) enriching said vertebrate host cells expressing said antibody or fragment thereof on the basis of their ability to bind to said desired antigen or ligand by separating cells that exhibit specific antigen or ligand binding from a non-binding cell population, thus selectively isolating strong antibody binders having high affinity to said desired antigen or ligand; and (d) isolating and identifying said at least one stably transduced vertebrate host cell expressing said antibody or fragment thereof specific for the desired antigen or ligand on its cell surface from the retrovirally transduced and enriched vertebrate host cells.
Hydraulic damper comprising a damper piston fixed to a main rod, sliding in an inner tube, moving during compression in an axial direction called the front, and a compensation chamber surrounding the inner tube, receiving the fluid displaced by the inlet of the main rod in the damper, the damper piston supporting towards the front an end stop rod comprising at its end a stop piston entering a stop tube having outlet bores fluid chamber (18, 34), characterized in that the compensation chamber comprises a plug sliding axially around the inner tube with a braking system, forming a float moving according to the level of fluid in this compensation chamber, which acts according to its displacement on adjustable bores of the stop tube.
A zinc salt of 2-[3-[ -3-[[2-chloro-3-(trifluoromethyl)phenyl]methyl-(2,2-diphenylethyl)amino]butoxy]phenyl]acetic acid (Compound 1), wherein the zinc salt is a 2:1 (Compound 1:zinc) salt.
Method for the determination of the glycosylation pattern of a human or humanized immunoglobulin of the subclass IgG1 or IgG2 or IgG4 or a Fab 2 -fragment thereof comprising the following steps: a) cleaving said immunoglobulin into fragments by enzymatic digestion with the enzyme IdeS and treating said immunoglobulin with carboxypeptidase B, b) treating said enzymatically cleaved immunoglobulin with formic acid and TCEP, c) separating the fragments of said immunoglobulin obtained by the enzymatic digestion by reversed phase high performance liquid chromatography, d) subjecting the separated fragments of said immunoglobulin obtained in step c) to a mass spectrometric analysis wherein the mass spectrometric analysis is performed in the absence of trifluoro acetic acid, and e) determining the glycosylation pattern of said immunoglobulin from the mass spectrometric data obtained in step d). a) cleaving said immunoglobulin into fragments by enzymatic digestion with the enzyme IdeS and treating said immunoglobulin with carboxypeptidase B, b) treating said enzymatically cleaved immunoglobulin with formic acid and TCEP, c) separating the fragments of said immunoglobulin obtained by the enzymatic digestion by reversed phase high performance liquid chromatography, d) subjecting the separated fragments of said immunoglobulin obtained in step c) to a mass spectrometric analysis wherein the mass spectrometric analysis is performed in the absence of trifluoro acetic acid, and e) determining the glycosylation pattern of said immunoglobulin from the mass spectrometric data obtained in step d).
A superconducting wire rod connection structure comprising: first and second superconducting wire rods (15A, 15B), wherein, the first and second superconducting wire rods (15A, 15B) are formed by layering a base material, an intermediate layer, and a superconducting conductor layer, the base materials of the first and second superconducting wire rods (15A, 15B) are joined to each other, and the superconducting conductor layers of the first and second superconducting wire rods (15A, 15B) are connected by a connection wire rod including a superconducting conductor layer, and a separating portion in which connection ends of the first and second superconducting wire rods (15A, 15B) with the base materials joined to each other are separated from the connection wire rod characterized in that the superconducting conductor layers of the first and second superconducting wire rods (15A, 15B) and the superconducting conductor layer of the connection wire rod are joined facing each other; and an opening is provided on a surface of the base material on a side opposite of the superconducting conductor layer within either one or both joining ranges between the first and second superconducting wire rods (15A, 15B) and the connection wire rod. first and second superconducting wire rods (15A, 15B), wherein, the first and second superconducting wire rods (15A, 15B) are formed by layering a base material, an intermediate layer, and a superconducting conductor layer, the base materials of the first and second superconducting wire rods (15A, 15B) are joined to each other, and the superconducting conductor layers of the first and second superconducting wire rods (15A, 15B) are connected by a connection wire rod including a superconducting conductor layer, and a separating portion in which connection ends of the first and second superconducting wire rods (15A, 15B) with the base materials joined to each other are separated from the connection wire rod characterized in that the superconducting conductor layers of the first and second superconducting wire rods (15A, 15B) and the superconducting conductor layer of the connection wire rod are joined facing each other; and an opening is provided on a surface of the base material on a side opposite of the superconducting conductor layer within either one or both joining ranges between the first and second superconducting wire rods (15A, 15B) and the connection wire rod.
A radial bearing assembly comprising: a polycrystalline diamond element that includes an engagement surface; a diamond reactive material that includes an opposing engagement surface, wherein the diamond reactive material is a metal or metal alloy that comprises at least 2 wt.% of a diamond catalyst or diamond solvent based on a total weight of the metal or metal alloy, wherein the engagement surface of the polycrystalline diamond element is in sliding contact with the opposing engagement surface. a polycrystalline diamond element that includes an engagement surface; a diamond reactive material that includes an opposing engagement surface, wherein the diamond reactive material is a metal or metal alloy that comprises at least 2 wt.% of a diamond catalyst or diamond solvent based on a total weight of the metal or metal alloy, wherein the engagement surface of the polycrystalline diamond element is in sliding contact with the opposing engagement surface.
A method of erecting a wind energy plant, in particular with a hub height of at least 140 m, comprising a tower and large components attached to the tower top, the large components comprising a nacelle, a generator and a rotor having at least one rotor blade, wherein: a light-duty lifting device designed for lighter-weight loads is used for erecting the tower and is attached to the tower during construction, in order to gain height together with the tower during construction thereof, a heavy-duty lifting device suitable for the installation of the large components is transported to the tower, is lifted to the tower top by means of the light-duty lifting device and is temporarily coupled to the tower to form a heavy-duty crane, using the tower as a crane mast, the heavy-duty lifting device being transported to the tower top while being supported against the tower, being temporarily fastened to the tower top, and, after installation of the large components, being dismounted from the tower again while being supported against the tower, at least one of the large components, namely at leastl the generator, is lifted to the tower top by the heavy-duty lifting device and installed there, the heavy-duty lifting device is decoupled from the tower again after installation of the large components. a light-duty lifting device designed for lighter-weight loads is used for erecting the tower and is attached to the tower during construction, in order to gain height together with the tower during construction thereof, a heavy-duty lifting device suitable for the installation of the large components is transported to the tower, is lifted to the tower top by means of the light-duty lifting device and is temporarily coupled to the tower to form a heavy-duty crane, using the tower as a crane mast, the heavy-duty lifting device being transported to the tower top while being supported against the tower, being temporarily fastened to the tower top, and, after installation of the large components, being dismounted from the tower again while being supported against the tower, at least one of the large components, namely at leastl the generator, is lifted to the tower top by the heavy-duty lifting device and installed there, the heavy-duty lifting device is decoupled from the tower again after installation of the large components.
A shaving unit comprising: - a housing; - a guard stationary relative to the housing; - a cutting edge arranged at a distance from the guard and stationary relative to the housing; - a hair-entry opening bound by the guard and the cutting edge; - a drum arranged within the housing, the drum being configured to be driven rotatingly about a longitudinal axis thereof; - at least one counter cutting edge arranged on the drum for cooperating with the cutting edge during rotation of the drum about the longitudinal axis for shearing or cutting hairs entering the hair-entry opening; and - a lubricating arrangement comprising: a liquid reservoir configured for receiving a lubricant, and a feed mechanism including a lubricating element (39, 46) arranged adjacent to the drum in a position such as to engage the at least one counter cutting edge during rotation of the drum about the longitudinal axis for providing a lubricating film to the at least one counter cutting edge. - a housing; - a guard stationary relative to the housing; - a cutting edge arranged at a distance from the guard and stationary relative to the housing; - a hair-entry opening bound by the guard and the cutting edge; - a drum arranged within the housing, the drum being configured to be driven rotatingly about a longitudinal axis thereof; - at least one counter cutting edge arranged on the drum for cooperating with the cutting edge during rotation of the drum about the longitudinal axis for shearing or cutting hairs entering the hair-entry opening; and - a lubricating arrangement comprising: a liquid reservoir configured for receiving a lubricant, and a feed mechanism including a lubricating element (39, 46) arranged adjacent to the drum in a position such as to engage the at least one counter cutting edge during rotation of the drum about the longitudinal axis for providing a lubricating film to the at least one counter cutting edge. a liquid reservoir configured for receiving a lubricant, and a feed mechanism including a lubricating element (39, 46) arranged adjacent to the drum in a position such as to engage the at least one counter cutting edge during rotation of the drum about the longitudinal axis for providing a lubricating film to the at least one counter cutting edge.
A method of identifying a subject having chronic lymphocytic leukemia or acute lymphoblastic leukemia that is responsive to treatment with an anti-CD19 antibody, said method comprising: a. providing a blood sample obtained from said subject prior to treatment with said anti-CD19 antibody, b. determining the level of at least one biomarker in said sample selected from the group consisting of: i. peripheral NK cell count, and ii. CD16 expression levels on peripheral NK cells, c. comparing the level of said at least one biomarker in said sample to a predetermined cut off level, wherein levels of said at least one biomarker at or above the predetermined cut off level is indicative of a subject who would benefit from treatment with an anti-CD19 antibody. a. providing a blood sample obtained from said subject prior to treatment with said anti-CD19 antibody, b. determining the level of at least one biomarker in said sample selected from the group consisting of: i. peripheral NK cell count, and ii. CD16 expression levels on peripheral NK cells, i. peripheral NK cell count, and ii. CD16 expression levels on peripheral NK cells, c. comparing the level of said at least one biomarker in said sample to a predetermined cut off level, wherein levels of said at least one biomarker at or above the predetermined cut off level is indicative of a subject who would benefit from treatment with an anti-CD19 antibody.
A low-consumption lamp with light sources of the LED type, comprising a supporting structure (2; 60) made of a material that dissipates heat, to which a flexible element is connected which supports LED sources, and a wiring for the electric power supply of said LED sources, said flexible element being associated with said supporting structure (2; 60) by means of a layer of heat-conducting adhesive, characterised in that the flexible element also supports a power switch and a capacitive luminous intensity regulator activatable by touching.
A computing system comprising: a plurality of processors; a cache configured to be accessed by at least two of the plurality of processors; a cache controller coupled to the cache; a persistent memory coupled to the cache controller; wherein a first thread of a plurality of threads executed by a first processor of the plurality of processors is configured to send a given data block to the persistent memory for storage via the cache controller; and wherein the cache controller is configured to: in response to receiving the given data block and determining a coherence state corresponding to the given data block does not already prevent read access, increment a respective per-thread count for the first thread; prevent any read access of the copy of the given data block in said cache, in response to detecting the given data block is to be sent to be stored in the persistent memory; and in response to receiving a corresponding acknowledgment that the given data block is stored in the persistent memory, decrement the respective per-thread count for the first thread. a plurality of processors; a cache configured to be accessed by at least two of the plurality of processors; a cache controller coupled to the cache; a persistent memory coupled to the cache controller; wherein a first thread of a plurality of threads executed by a first processor of the plurality of processors is configured to send a given data block to the persistent memory for storage via the cache controller; and wherein the cache controller is configured to: in response to receiving the given data block and determining a coherence state corresponding to the given data block does not already prevent read access, increment a respective per-thread count for the first thread; prevent any read access of the copy of the given data block in said cache, in response to detecting the given data block is to be sent to be stored in the persistent memory; and in response to receiving a corresponding acknowledgment that the given data block is stored in the persistent memory, decrement the respective per-thread count for the first thread. in response to receiving the given data block and determining a coherence state corresponding to the given data block does not already prevent read access, increment a respective per-thread count for the first thread; prevent any read access of the copy of the given data block in said cache, in response to detecting the given data block is to be sent to be stored in the persistent memory; and in response to receiving a corresponding acknowledgment that the given data block is stored in the persistent memory, decrement the respective per-thread count for the first thread.
A method of producing a purified argon product stream (174, 195) in a cryogenic air separation unit, the method comprising the steps of: separating argon from an oxygen-argon containing stream within a lower pressure column of the cryogenic air separation unit using an argon rectification column arrangement (90, 56) disposed within the lower pressure column, the separation of the argon from the oxygen-argon containing stream producing an impure argon stream having between about and 4% and 25% of oxygen impurities; characterized by warming the impure argon stream via indirect heat exchange in an argon recovery heat exchanger (152, 173) against a purified argon stream from a pressure swing adsorption system, a waste stream from the pressure swing adsorption system and against a balancing air stream of purified, compressed air from an incoming air purification and compression train (24, 26) of the cryogenic air separation unit; pressurizing the warmed impure argon stream; and purifying the warmed, pressurized, impure argon stream by introducing the warm, pressurized impure argon stream into the pressure swing adsorption system to produce the purified argon product stream and the waste gas stream; wherein the impure argon stream is an impure gaseous argon stream diverted from an upper location of the argon rectification column arrangement disposed within the lower pressure column. separating argon from an oxygen-argon containing stream within a lower pressure column of the cryogenic air separation unit using an argon rectification column arrangement (90, 56) disposed within the lower pressure column, the separation of the argon from the oxygen-argon containing stream producing an impure argon stream having between about and 4% and 25% of oxygen impurities; characterized by warming the impure argon stream via indirect heat exchange in an argon recovery heat exchanger (152, 173) against a purified argon stream from a pressure swing adsorption system, a waste stream from the pressure swing adsorption system and against a balancing air stream of purified, compressed air from an incoming air purification and compression train (24, 26) of the cryogenic air separation unit; pressurizing the warmed impure argon stream; and purifying the warmed, pressurized, impure argon stream by introducing the warm, pressurized impure argon stream into the pressure swing adsorption system to produce the purified argon product stream and the waste gas stream; wherein the impure argon stream is an impure gaseous argon stream diverted from an upper location of the argon rectification column arrangement disposed within the lower pressure column.
A slide fastener having a waterproof structure, comprising: a pair of left and right fastener stringers, each of the fastener stringers including a pair of left and right waterproof fastener tapes and a pair of left and right fastener element rows having a plurality of fastener elements along the side ends of the fastener tapes opposite to each other; a slider for engaging and disengaging the left and right fastener elements with and from each other; and a bottom stopper (4, 104) which is formed over the left and right fastener tapes at lower ends of the rows of the fastener elements, wherein each of the fastener elements includes an engaging head, a base, a neck for connecting between the engaging head and the base, and a pair of shoulders which extend from both sides of the neck in the shape of wing pieces, and wherein said bottom stopper (4, 104) includes a depression (40, 140) depressed downward in the upper side of the bottom stopper (4,104), characterized in that said bottom stopper (4, 104) covers at least an entire lower half of a first fastener element which is the fastener element at the lowest end or near the lowest end of one of the fastener element rows, and also covers at least a portion of a second fastener element which is the fastener element adjacent upward to the first fastener element, the first fastener element being of one of the left and right fastener element rows and the second fastener element being of the other one of the fastener element rows. a pair of left and right fastener stringers, each of the fastener stringers including a pair of left and right waterproof fastener tapes and a pair of left and right fastener element rows having a plurality of fastener elements along the side ends of the fastener tapes opposite to each other; a slider for engaging and disengaging the left and right fastener elements with and from each other; and a bottom stopper (4, 104) which is formed over the left and right fastener tapes at lower ends of the rows of the fastener elements, wherein each of the fastener elements includes an engaging head, a base, a neck for connecting between the engaging head and the base, and a pair of shoulders which extend from both sides of the neck in the shape of wing pieces, and wherein said bottom stopper (4, 104) includes a depression (40, 140) depressed downward in the upper side of the bottom stopper (4,104), characterized in that said bottom stopper (4, 104) covers at least an entire lower half of a first fastener element which is the fastener element at the lowest end or near the lowest end of one of the fastener element rows, and also covers at least a portion of a second fastener element which is the fastener element adjacent upward to the first fastener element, the first fastener element being of one of the left and right fastener element rows and the second fastener element being of the other one of the fastener element rows.