A magnetic resonance imaging apparatus comprising: a scanner comprising a static magnetic field coil assembly configured to form a static magnetic field in a bore, a gradient coil assembly configured to apply a gradient to the formed static magnetic field, and a radio frequency, RF, coil assembly configured to apply an RF pulse to an object located in the bore; a sequence controller configured to control the scanner to apply an unsaturation pulse sequence without any pre-applied RF pulse, to a first region of the object and a saturation pulse sequence, which includes a pre-applied RF pulse, to the first region of the object, wherein the pre-applied RF pulse is an RF pulse that is applied for saturation before starting a pulse sequence used to acquire a magnetic resonance image; and a data processor configured to acquire a first image by receiving a magnetic resonance signal from the first region of the object to which the unsaturation pulse sequence is applied, to acquire a second image by receiving a magnetic resonance signal from the first region of the object to which the saturation pulse sequence is applied, and to obtain a difference image between the first image and the second image; wherein the first region corresponds to a slice in the object, and the unsaturation pulse sequence and the saturation pulse sequence are slice selective sequences; characterized in that the pre-applied RF pulse of the saturation pulse sequence is configured to saturate at least one region of interest, ROI, in the slice; and the data processor is configured to extract the saturated at least one ROI via subtraction of the second image from the first image. a scanner comprising a static magnetic field coil assembly configured to form a static magnetic field in a bore, a gradient coil assembly configured to apply a gradient to the formed static magnetic field, and a radio frequency, RF, coil assembly configured to apply an RF pulse to an object located in the bore; a sequence controller configured to control the scanner to apply an unsaturation pulse sequence without any pre-applied RF pulse, to a first region of the object and a saturation pulse sequence, which includes a pre-applied RF pulse, to the first region of the object, wherein the pre-applied RF pulse is an RF pulse that is applied for saturation before starting a pulse sequence used to acquire a magnetic resonance image; and a data processor configured to acquire a first image by receiving a magnetic resonance signal from the first region of the object to which the unsaturation pulse sequence is applied, to acquire a second image by receiving a magnetic resonance signal from the first region of the object to which the saturation pulse sequence is applied, and to obtain a difference image between the first image and the second image; wherein the first region corresponds to a slice in the object, and the unsaturation pulse sequence and the saturation pulse sequence are slice selective sequences; characterized in that the pre-applied RF pulse of the saturation pulse sequence is configured to saturate at least one region of interest, ROI, in the slice; and the data processor is configured to extract the saturated at least one ROI via subtraction of the second image from the first image.