A biological particle analysis method, comprising: a staining step implemented by fluorescence staining a liquid specimen having a plurality of biological particles through a fluorescence staining process, so that at least one of the biological particles becomes a fluorescence color and is defined as at least one target biological particle; an analyzing step implemented by accommodating the liquid specimen being fluorescence stained into a pico-droplet generator, and using a camera device to take a real-time image of the liquid specimen in the pico-droplet generator, wherein the pico-droplet generator includes a hollow needle for receiving the liquid specimen therein, a container and a top piezoelectric member disposed on the container, the container receives the liquid specimen, and the hollow needle is in fluid communication with the container by being connected to a bottom side of the container, wherein the top piezoelectric member is configured to vibrate the container for enabling the biological particles in the container to be arranged along a predetermined path of the hollow needle, and wherein the camera device corresponds in position to the hollow needle, and the camera device in the analyzing step is used to take the real-time image of the liquid specimen in a free end of the hollow needle; a capturing step implemented by using the pico-droplet generator to output a target pico-droplet having the at least one target biological particle onto a biochip according to the real-time image, wherein the at least one target biological particle in the target pico-droplet is captured by the biochip; a washing step implemented by removing the fluorescent color of the at least one target biological particle in the target pico-droplet captured by the biochip through a washing process; and a characterization expressing step implemented by fluorescence staining the at least one target biological particle captured by the biochip for N number of times through the fluorescence staining process and the washing process, and using a recording device to obtain a plurality of fluorescence images respectively corresponding to N kinds of biological characterization expressions, wherein N is a positive integer within a range from 2 to 50 and wherein each fluorescence staining of the at least one target biological particle is related to one of the N kinds of biological characterization expressions. a staining step implemented by fluorescence staining a liquid specimen having a plurality of biological particles through a fluorescence staining process, so that at least one of the biological particles becomes a fluorescence color and is defined as at least one target biological particle; an analyzing step implemented by accommodating the liquid specimen being fluorescence stained into a pico-droplet generator, and using a camera device to take a real-time image of the liquid specimen in the pico-droplet generator, wherein the pico-droplet generator includes a hollow needle for receiving the liquid specimen therein, a container and a top piezoelectric member disposed on the container, the container receives the liquid specimen, and the hollow needle is in fluid communication with the container by being connected to a bottom side of the container, wherein the top piezoelectric member is configured to vibrate the container for enabling the biological particles in the container to be arranged along a predetermined path of the hollow needle, and wherein the camera device corresponds in position to the hollow needle, and the camera device in the analyzing step is used to take the real-time image of the liquid specimen in a free end of the hollow needle; a capturing step implemented by using the pico-droplet generator to output a target pico-droplet having the at least one target biological particle onto a biochip according to the real-time image, wherein the at least one target biological particle in the target pico-droplet is captured by the biochip; a washing step implemented by removing the fluorescent color of the at least one target biological particle in the target pico-droplet captured by the biochip through a washing process; and a characterization expressing step implemented by fluorescence staining the at least one target biological particle captured by the biochip for N number of times through the fluorescence staining process and the washing process, and using a recording device to obtain a plurality of fluorescence images respectively corresponding to N kinds of biological characterization expressions, wherein N is a positive integer within a range from 2 to 50 and wherein each fluorescence staining of the at least one target biological particle is related to one of the N kinds of biological characterization expressions.