Isolation device for providing a sterile operation chamber above a skin region of a patient, in particular for performing a surgical procedure, said isolation device comprising - a first inflatable bag, the first inflatable bag delimiting said sterile operation chamber against the ambient environment, said first inflatable bag comprising: a first and a second access opening (105, 106) to allow a user to insert a first and a second arm into the operation chamber; and a chamber operation opening to allow access to said skin region of the patient out of the operation chamber; and a window for observing handling operations inside said sterile operation chamber wherein the first inflatable bag is coupled to a support frame (110, 120), the support frame having a transport configuration and an operation configuration, wherein in the transport configuration, the first inflatable bag is confined, such as to have compact dimensions; in the operation configuration, the first inflatable bag is extended, such as to form said sterile operation chamber; characterized in further comprising a device for introducing sterile instruments into an operation chamber or an instrument chamber, comprising: an introduction frame; a first roller (131; 520) and a second roller (132; 530) arranged rotatable around their respective longitudinal axis, and attached to said introduction frame, such that the longitudinal axis of the first roller and the longitudinal axis of the second roller are parallel, wherein the first roller and the second roller are further attached to said introduction frame such as to be movable along an axis extending perpendicular to their respective longitudinal axis; wherein a distance between the first roller and the second roller is determined by a position of a movement of the first and second roller along the axis extending perpendicular to the longitudinal axes, wherein said first and second rollers are biased versus each other to contact each other and said first and second roller can be moved along said axis to increase said distance to allow insertion of an instrument through a gap provided by said distance, wherein said introduction frame is attached to said first inflatable bag of said isolation device. - a first inflatable bag, the first inflatable bag delimiting said sterile operation chamber against the ambient environment, a first and a second access opening (105, 106) to allow a user to insert a first and a second arm into the operation chamber; and a chamber operation opening to allow access to said skin region of the patient out of the operation chamber; and a window for observing handling operations inside said sterile operation chamber wherein the first inflatable bag is coupled to a support frame (110, 120), the support frame having a transport configuration and an operation configuration, wherein in the transport configuration, the first inflatable bag is confined, such as to have compact dimensions; in the operation configuration, the first inflatable bag is extended, such as to form said sterile operation chamber; characterized in further comprising a device for introducing sterile instruments into an operation chamber or an instrument chamber, comprising: an introduction frame; a first roller (131; 520) and a second roller (132; 530) arranged rotatable around their respective longitudinal axis, and attached to said introduction frame, such that the longitudinal axis of the first roller and the longitudinal axis of the second roller are parallel, wherein the first roller and the second roller are further attached to said introduction frame such as to be movable along an axis extending perpendicular to their respective longitudinal axis; wherein a distance between the first roller and the second roller is determined by a position of a movement of the first and second roller along the axis extending perpendicular to the longitudinal axes, wherein said first and second rollers are biased versus each other to contact each other and said first and second roller can be moved along said axis to increase said distance to allow insertion of an instrument through a gap provided by said distance, wherein said introduction frame is attached to said first inflatable bag of said isolation device. an introduction frame; a first roller (131; 520) and a second roller (132; 530) arranged rotatable around their respective longitudinal axis, and attached to said introduction frame, such that the longitudinal axis of the first roller and the longitudinal axis of the second roller are parallel, wherein the first roller and the second roller are further attached to said introduction frame such as to be movable along an axis extending perpendicular to their respective longitudinal axis; wherein a distance between the first roller and the second roller is determined by a position of a movement of the first and second roller along the axis extending perpendicular to the longitudinal axes, wherein said first and second rollers are biased versus each other to contact each other and said first and second roller can be moved along said axis to increase said distance to allow insertion of an instrument through a gap provided by said distance, wherein said introduction frame is attached to said first inflatable bag of said isolation device.