Method of using a device (100; 201; 901; 2010; 2011; 2012; 2013; 2014; 2015; 2016; 2017; 2018; 2019) for in-vitro modelling in-vivo tissues of organs, the device (100; 201; 901; 2010; 2011; 2012; 2013; 2014; 2015; 2016; 2017; 2018; 2019) comprising a first body portion (207; 2070; 2071; 2072; 2073; 2074; 2075 2076; 2077; 2078; 2079) with at least one access chamber (213; 2130; 2131; 2132; 2133; 2134; 2135; 2136; 2137; 2138; 2139), a second body portion (205; 2050; 2051; 2052; 2053; 2054; 2055; 2056; 2057; 2058; 2059) with at least one culturing chamber (221; 2210; 2211; 2212; 2213; 2214; 2215; 2216; 2217; 2218; 2219), and a culturing membrane (206; 2060; 2061; 2062; 2063; 2064; 2065; 2066; 2067; 2068; 2069) dividing the at least one access chamber (213; 2130; 2131; 2132; 2133; 2134; 2135; 2136; 2137; 2138; 2139) from the culturing chamber (221; 2210; 2211; 2212; 2213; 2214; 2215; 2216; 2217; 2218; 2219) and a third body portion (203; 2030; 2031; 2032; 2033; 2054; 2035; 2036; 2037; 2038; 2039) with at least one actuation chamber (220; 2200; 2201; 2202; 2203; 2204; 2205; 2206; 2207; 2208; 2209), and an actuation membrane (204; 2040; 2041; 2042; 2043; 2044; 2045; 2046; 2047; 2048; 2049) dividing the at least one culturing chamber (221; 2210; 2211; 2212; 2213; 2214; 2215; 2216; 2217; 2218; 2219) from the at least one actuation chamber (220; 2200; 2201; 2202; 2203; 2204; 2205; 2206; 2207; 2208; 2209), the method comprising: flipping the first body portion (207; 2070; 2071; 2072; 2073; 2074; 2075 2076; 2077; 2078; 2079) and the second body portion (205; 2050; 2051; 2052; 2053; 2054; 2055; 2056; 2057; 2058; 2059) together with the culturing membrane (206; 2060; 2061; 2062; 2063; 2064; 2065; 2066; 2067; 2068; 2069) by 180°; and incubating cells by providing the cells on top of a first side of the culturing membrane (206; 2060; 2061; 2062; 2063; 2064; 2065; 2066; 2067; 2068; 2069) until the cells attach to the culturing membrane (206; 2060; 2061; 2062; 2063; 2064; 2065; 2066; 2067; 2068; 2069); turning down the first body portion (207; 2070; 2071; 2072; 2073; 2074; 2075 2076; 2077; 2078; 2079) and the second body portion (205; 2050; 2051; 2052; 2053; 2054; 2055; 2056; 2057; 2058; 2059) together with the culturing membrane (206; 2060; 2061; 2062; 2063; 2064; 2065; 2066; 2067; 2068; 2069) and assembling the first body portion (207; 2070; 2071; 2072; 2073; 2074; 2075 2076; 2077; 2078; 2079) and the second body portion (205; 2050; 2051; 2052; 2053; 2054; 2055; 2056; 2057; 2058; 2059) together with the culturing membrane (206; 2060; 2061; 2062; 2063; 2064; 2065; 2066; 2067; 2068; 2069) with the third body portion (203; 2030; 2031; 2032; 2033; 2054; 2035; 2036; 2037; 2038; 2039) and the actuation membrane (204; 2040; 2041; 2042; 2043; 2044; 2045; 2046; 2047; 2048; 2049); and incubating further cells by providing the further cells on top of a second side of the culturing membrane (206; 2060; 2061; 2062; 2063; 2064; 2065; 2066; 2067; 2068; 2069) inside the at least one access chamber (213; 2130; 2131; 2132; 2133; 2134; 2135; 2136; 2137; 2138; 2139) until the further cells attach to the culturing membrane (206; 2060; 2061; 2062; 2063; 2064; 2065; 2066; 2067; 2068; 2069). flipping the first body portion (207; 2070; 2071; 2072; 2073; 2074; 2075 2076; 2077; 2078; 2079) and the second body portion (205; 2050; 2051; 2052; 2053; 2054; 2055; 2056; 2057; 2058; 2059) together with the culturing membrane (206; 2060; 2061; 2062; 2063; 2064; 2065; 2066; 2067; 2068; 2069) by 180°; and incubating cells by providing the cells on top of a first side of the culturing membrane (206; 2060; 2061; 2062; 2063; 2064; 2065; 2066; 2067; 2068; 2069) until the cells attach to the culturing membrane (206; 2060; 2061; 2062; 2063; 2064; 2065; 2066; 2067; 2068; 2069); turning down the first body portion (207; 2070; 2071; 2072; 2073; 2074; 2075 2076; 2077; 2078; 2079) and the second body portion (205; 2050; 2051; 2052; 2053; 2054; 2055; 2056; 2057; 2058; 2059) together with the culturing membrane (206; 2060; 2061; 2062; 2063; 2064; 2065; 2066; 2067; 2068; 2069) and assembling the first body portion (207; 2070; 2071; 2072; 2073; 2074; 2075 2076; 2077; 2078; 2079) and the second body portion (205; 2050; 2051; 2052; 2053; 2054; 2055; 2056; 2057; 2058; 2059) together with the culturing membrane (206; 2060; 2061; 2062; 2063; 2064; 2065; 2066; 2067; 2068; 2069) with the third body portion (203; 2030; 2031; 2032; 2033; 2054; 2035; 2036; 2037; 2038; 2039) and the actuation membrane (204; 2040; 2041; 2042; 2043; 2044; 2045; 2046; 2047; 2048; 2049); and incubating further cells by providing the further cells on top of a second side of the culturing membrane (206; 2060; 2061; 2062; 2063; 2064; 2065; 2066; 2067; 2068; 2069) inside the at least one access chamber (213; 2130; 2131; 2132; 2133; 2134; 2135; 2136; 2137; 2138; 2139) until the further cells attach to the culturing membrane (206; 2060; 2061; 2062; 2063; 2064; 2065; 2066; 2067; 2068; 2069).