An in vitro method for assessment of male reproductive health comprising: (a) assessing sperm DNA decondensation rate of a test sperm sample and comparing said rate to a control sperm DNA decondensation rate comprising: incubating the test sperm sample in a non-mammalian egg extract capable of supporting human sperm DNA decondensation and sperm DNA synthesis for a defined incubation period of 5 to 10 minutes; determining a test sperm DNA decondensation percentage from the percent of DNA decondensed in the test sperm sample; and comparing the test sperm DNA decondensation percentage to a control sperm DNA decondensation percentage to provide a test sperm DNA decondensation value percentage assessment, wherein a test sperm decondensation percentage that is 120% or more than the control sperm DNA decondensation percentage after the incubation period identifies a test sample demonstrating an accelerated sperm DNA decondensation value; and (b) assessing sperm DNA fragmentation (SDFA) present in the test sperm sample comprising: determining a ratio of broken DNA to unbroken DNA in the test sperm sample to provide a test sperm DNA fragmentation index, wherein a test sperm sample having an accelerated sperm DNA decondensation value and a DFI of 30 or greater provides an assessment of male reproductive health of the male animal. (a) assessing sperm DNA decondensation rate of a test sperm sample and comparing said rate to a control sperm DNA decondensation rate comprising: incubating the test sperm sample in a non-mammalian egg extract capable of supporting human sperm DNA decondensation and sperm DNA synthesis for a defined incubation period of 5 to 10 minutes; determining a test sperm DNA decondensation percentage from the percent of DNA decondensed in the test sperm sample; and comparing the test sperm DNA decondensation percentage to a control sperm DNA decondensation percentage to provide a test sperm DNA decondensation value percentage assessment, wherein a test sperm decondensation percentage that is 120% or more than the control sperm DNA decondensation percentage after the incubation period identifies a test sample demonstrating an accelerated sperm DNA decondensation value; and incubating the test sperm sample in a non-mammalian egg extract capable of supporting human sperm DNA decondensation and sperm DNA synthesis for a defined incubation period of 5 to 10 minutes; determining a test sperm DNA decondensation percentage from the percent of DNA decondensed in the test sperm sample; and comparing the test sperm DNA decondensation percentage to a control sperm DNA decondensation percentage to provide a test sperm DNA decondensation value percentage assessment, wherein a test sperm decondensation percentage that is 120% or more than the control sperm DNA decondensation percentage after the incubation period identifies a test sample demonstrating an accelerated sperm DNA decondensation value; and (b) assessing sperm DNA fragmentation (SDFA) present in the test sperm sample comprising: determining a ratio of broken DNA to unbroken DNA in the test sperm sample to provide a test sperm DNA fragmentation index, wherein a test sperm sample having an accelerated sperm DNA decondensation value and a DFI of 30 or greater provides an assessment of male reproductive health of the male animal. determining a ratio of broken DNA to unbroken DNA in the test sperm sample to provide a test sperm DNA fragmentation index, wherein a test sperm sample having an accelerated sperm DNA decondensation value and a DFI of 30 or greater provides an assessment of male reproductive health of the male animal.