A separation container for production of platelet-rich plasma from blood, which is sterilisable and comprising: - a test tube for a bench centrifuge, the test tube having a relative conical bottom portion, a first opening which is opposite the conical bottom portion, wherein the test tube internally defines a relative first housing; - a cap which is engageable with the first opening of the test tube, the cap having: a first, a second and a third hole which are through-holes; - a tube which originates from the cap at the first needle free connector so that, when the cap is engaged with the first opening, the tube is arranged internally of the first housing with a relative terminal portion having a relative terminal end, arranged distally of the cap and at the conical bottom portion of the test tube, characterized in that the separation container further comprises: a first and a second needle free connector (15, 16) respectively engaged, in the first and in the second hole in order to close them, wherein, when the cap is engaged with the first opening, each of the first and second needle free connectors (15, 16) is arranged in such a way that a relative compression valve is arranged distally to the test tube; a filter engaged in the third hole and having pores of dimensions that are equal to or smaller than 0.4 μm in order to allow only passage of microbiologically filtered air in conjunction with the transfer of a liquid through the first and/or the second needle free connector (15, 16) when the relative compression valve is compressed; - the separation container being further characterized in that said terminal portion of the tube is tapered towards the relative terminal in order to obtain a Venturi effect when a liquid is introduced into the test tube, through the first needle free connector and the tube, to enable re-mixing of a sediment of particles and of a supernatant liquid when contained in the separation container. - a test tube for a bench centrifuge, the test tube having a relative conical bottom portion, a first opening which is opposite the conical bottom portion, wherein the test tube internally defines a relative first housing; - a cap which is engageable with the first opening of the test tube, the cap having: a first, a second and a third hole which are through-holes; - a tube which originates from the cap at the first needle free connector so that, when the cap is engaged with the first opening, the tube is arranged internally of the first housing with a relative terminal portion having a relative terminal end, arranged distally of the cap and at the conical bottom portion of the test tube, characterized in that the separation container further comprises: a first and a second needle free connector (15, 16) respectively engaged, in the first and in the second hole in order to close them, wherein, when the cap is engaged with the first opening, each of the first and second needle free connectors (15, 16) is arranged in such a way that a relative compression valve is arranged distally to the test tube; a filter engaged in the third hole and having pores of dimensions that are equal to or smaller than 0.4 μm in order to allow only passage of microbiologically filtered air in conjunction with the transfer of a liquid through the first and/or the second needle free connector (15, 16) when the relative compression valve is compressed; a first and a second needle free connector (15, 16) respectively engaged, in the first and in the second hole in order to close them, wherein, when the cap is engaged with the first opening, each of the first and second needle free connectors (15, 16) is arranged in such a way that a relative compression valve is arranged distally to the test tube; a filter engaged in the third hole and having pores of dimensions that are equal to or smaller than 0.4 μm in order to allow only passage of microbiologically filtered air in conjunction with the transfer of a liquid through the first and/or the second needle free connector (15, 16) when the relative compression valve is compressed; - the separation container being further characterized in that said terminal portion of the tube is tapered towards the relative terminal in order to obtain a Venturi effect when a liquid is introduced into the test tube, through the first needle free connector and the tube, to enable re-mixing of a sediment of particles and of a supernatant liquid when contained in the separation container.