A method for determining the quantity of glycated haemoglobin (HbA1c) relative to the total concentration of haemoglobin in a blood sample consisting of less than 200μl blood, the method comprising the steps of: a. providing a fluorophore comprising a ligand capable of binding HbA1c, b. providing a buffered liquid, c. measuring the transmission (TRT 0) of the buffered liquid at time T 0 at a wavelength of approximately 570nm and optionally measuring the fluorescence (FLT 0) of the buffered liquid at a wavelength at which the fluorophore emits fluorescent light when excited, d. adding the fluorophore to the buffered liquid, thereby creating a buffered reaction liquid, and optionally measuring the fluorescence (FLT 1) of the buffered reaction liquid at time T 1 at the wavelength X, e. adding the sample to the buffered reaction liquid at time T 2, whereby a first detection liquid is formed comprising a fluorescence complex comprising the fluorophore and HbA1c, and measuring the fluorescence (FLT 3,4) at the wavelength X of the first detection liquid at one or more time points within the time interval T 3 - T 4, at which the change in fluorescence over time is >0, reflecting that less than all HbA1c in the sample is part of a fluorescence complex in the detection liquid, f. adding a haemoglobin-binding agent to the first detection liquid at time T 5, thereby creating a second detection liquid comprising a complex comprising the haemoglobin-binding agent and haemoglobin, and measuring the transmission (TRT 6) of the second detection liquid at approximately 570nm at time T 6, g. determining the absorbance of the sample by dividing the transmission signal (TRT 6) with the transmission signal (TRTo), h. determining the relative quantity of HbA1c by dividing the fluorescence signal (FLT 3,4), with the absorbance determined in step g), and comparing the obtained results with an internal standard. a. providing a fluorophore comprising a ligand capable of binding HbA1c, b. providing a buffered liquid, c. measuring the transmission (TRT 0) of the buffered liquid at time T 0 at a wavelength of approximately 570nm and optionally measuring the fluorescence (FLT 0) of the buffered liquid at a wavelength at which the fluorophore emits fluorescent light when excited, d. adding the fluorophore to the buffered liquid, thereby creating a buffered reaction liquid, and optionally measuring the fluorescence (FLT 1) of the buffered reaction liquid at time T 1 at the wavelength X, e. adding the sample to the buffered reaction liquid at time T 2, whereby a first detection liquid is formed comprising a fluorescence complex comprising the fluorophore and HbA1c, and measuring the fluorescence (FLT 3,4) at the wavelength X of the first detection liquid at one or more time points within the time interval T 3 - T 4, at which the change in fluorescence over time is >0, reflecting that less than all HbA1c in the sample is part of a fluorescence complex in the detection liquid, f. adding a haemoglobin-binding agent to the first detection liquid at time T 5, thereby creating a second detection liquid comprising a complex comprising the haemoglobin-binding agent and haemoglobin, and measuring the transmission (TRT 6) of the second detection liquid at approximately 570nm at time T 6, g. determining the absorbance of the sample by dividing the transmission signal (TRT 6) with the transmission signal (TRTo), h. determining the relative quantity of HbA1c by dividing the fluorescence signal (FLT 3,4), with the absorbance determined in step g), and comparing the obtained results with an internal standard.