A transdermal microfluidic sampling system, comprising: a plurality of samplers (500, 501, 502) each sampler (500, 501, 502) configured to function as a single-use unit (500, 501, 502) for transdermally retrieving and transferring at least one analyte from the skin of a subject, wherein the single-use units (500, 501, 502) are comprised of a multilayered assembly of micromachined silicon wafers comprising a capillary subsystem for storage, passage and analysis of physiological fluids, the capillary subsystem comprising a reservoir comprising a physiologically compatible fluid that is adapted to transfer at least one analyte obtained transdermally from the skin of a subject, a transport capillary and an electronic controllable breakable seal (205, 405, 601) sealing said reservoir for retaining said physiologically compatible fluid in said reservoir prior to sampling analytes from a subject; each of the plurality of samplers further comprising a microheater (603; 703; 1006) arranged to be in close proximity to a subject's skin surface during use and configured for ablating a portion of the stratum corneum of the skin of the subject on demand to facilitate access to interstitial fluid from the subject's underlying viable epidermis; at least one detector system connected to each of the plurality of samplers for identifying and quantifying said at least one analyte separately for each of the plurality of samplers (500, 501, 502); and at least one logic module (103, 104) for controlling the operation of said plurality of samplers (500, 501, 502), wherein the at least one logic module (103, 104) is configured for (i) receiving and storing input data from said at least one detector, (ii) relating the input data to other data obtained from the subject, (iii) displaying output information, (iv) transmitting the output information to another system, and (v) controlling the operation of the at least one detector system. a plurality of samplers (500, 501, 502) each sampler (500, 501, 502) configured to function as a single-use unit (500, 501, 502) for transdermally retrieving and transferring at least one analyte from the skin of a subject, wherein the single-use units (500, 501, 502) are comprised of a multilayered assembly of micromachined silicon wafers comprising a capillary subsystem for storage, passage and analysis of physiological fluids, the capillary subsystem comprising a reservoir comprising a physiologically compatible fluid that is adapted to transfer at least one analyte obtained transdermally from the skin of a subject, a transport capillary and an electronic controllable breakable seal (205, 405, 601) sealing said reservoir for retaining said physiologically compatible fluid in said reservoir prior to sampling analytes from a subject; each of the plurality of samplers further comprising a microheater (603; 703; 1006) arranged to be in close proximity to a subject's skin surface during use and configured for ablating a portion of the stratum corneum of the skin of the subject on demand to facilitate access to interstitial fluid from the subject's underlying viable epidermis; at least one detector system connected to each of the plurality of samplers for identifying and quantifying said at least one analyte separately for each of the plurality of samplers (500, 501, 502); and at least one logic module (103, 104) for controlling the operation of said plurality of samplers (500, 501, 502), wherein the at least one logic module (103, 104) is configured for (i) receiving and storing input data from said at least one detector, (ii) relating the input data to other data obtained from the subject, (iii) displaying output information, (iv) transmitting the output information to another system, and (v) controlling the operation of the at least one detector system.