A process for enhanced recovery of a concentrated biological fluid from a crossflow filtration system, said crossflow filtration system comprising a biofluid container, a crossflow filtration filter, a feed flow line operably linked from said biofluid container to said crossflow filtration filter, a retentate flow line operably linked from said crossflow filtration filter to said biofluid container, a permeate flow line operably linked to said crossflow filtration filter, a recirculating pump in said feed flow line, a drain line operably linked to said feed flow line via a drain valve wherein said drain line is located between said recirculating pump and said crossflow filtration filter, a sterile air source, an internal pump operably linked to said retentate flow line and said sterile air source, wherein said internal pump is located near or at a terminus of the crossflow filtration system adjacent to said biofluid source container (1 a), wherein said internal pump is a positive displacement pump, such as a peristaltic pump, said process, after concentrating the biofluid in the biofluid source container by cycling the biofluid through the crossflow filtration system and collecting waste from the permeate line, comprising: a. discontinuing the pressurized biofluid flow from the recirculating pump, b. closing valves from and to the biofluid source container, c. opening said drain valve, d. inducing an internalized pressurized countercurrent air flow throughout the crossflow filtration system by activating the internal pump, and, e. recovering the concentrated biological fluid at the drain valve a biofluid container, a crossflow filtration filter, a feed flow line operably linked from said biofluid container to said crossflow filtration filter, a retentate flow line operably linked from said crossflow filtration filter to said biofluid container, a permeate flow line operably linked to said crossflow filtration filter, a recirculating pump in said feed flow line, a drain line operably linked to said feed flow line via a drain valve wherein said drain line is located between said recirculating pump and said crossflow filtration filter, a sterile air source, an internal pump operably linked to said retentate flow line and said sterile air source, wherein said internal pump is located near or at a terminus of the crossflow filtration system adjacent to said biofluid source container (1 a), wherein said internal pump is a positive displacement pump, such as a peristaltic pump, said process, after concentrating the biofluid in the biofluid source container by cycling the biofluid through the crossflow filtration system and collecting waste from the permeate line, comprising: a. discontinuing the pressurized biofluid flow from the recirculating pump, b. closing valves from and to the biofluid source container, c. opening said drain valve, d. inducing an internalized pressurized countercurrent air flow throughout the crossflow filtration system by activating the internal pump, and, e. recovering the concentrated biological fluid at the drain valve a. discontinuing the pressurized biofluid flow from the recirculating pump, b. closing valves from and to the biofluid source container, c. opening said drain valve, d. inducing an internalized pressurized countercurrent air flow throughout the crossflow filtration system by activating the internal pump, and, e. recovering the concentrated biological fluid at the drain valve