Method for the detection of the following mycotoxins and metabolites in broiler chickens or pigs, - Aflatoxin B1, - Aflatoxin B2, - Aflatoxin M1, - Fumonisin, - Fumonisin, - Ochratoxin A, - Deoxynivalenol, - T2 toxin, - HT-2-toxin, - Zearalenone, said method comprising: - collecting the blood of broiler chickens or pigs as a dried blood sample; - preparing the dried blood sample for analysis; - analyzing the prepared dried blood sample by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in two steps: a) detecting in a LC-MS/MS step, - the reversed phase LC process using as mobile phase, a mixture of acetic acid, acetonitrile and water, the proportion of the acetonitrile solution over the water solution gradually increasing during the process, and at the end diminishing to recondition the LC column, - the mass spectrometer operating in negative electro-spray ionization mode, and b) detecting in another LC-MS/MS step, - the reversed phase LC process using as mobile phase, a mixture of ammonium formate, formic acid, water and methanol, the proportion of the methanol solution over the water solution gradually increasing during the process, and at the end diminishing to recondition the LC column, - the mass spectrometer operating in positive electro-spray ionization mode. - Aflatoxin B1, - Aflatoxin B2, - Aflatoxin M1, - Fumonisin, - Fumonisin, - Ochratoxin A, - Deoxynivalenol, - T2 toxin, - HT-2-toxin, - Zearalenone, - collecting the blood of broiler chickens or pigs as a dried blood sample; - preparing the dried blood sample for analysis; - analyzing the prepared dried blood sample by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in two steps: a) detecting in a LC-MS/MS step, - the reversed phase LC process using as mobile phase, a mixture of acetic acid, acetonitrile and water, the proportion of the acetonitrile solution over the water solution gradually increasing during the process, and at the end diminishing to recondition the LC column, - the mass spectrometer operating in negative electro-spray ionization mode, and b) detecting in another LC-MS/MS step, - the reversed phase LC process using as mobile phase, a mixture of ammonium formate, formic acid, water and methanol, the proportion of the methanol solution over the water solution gradually increasing during the process, and at the end diminishing to recondition the LC column, - the mass spectrometer operating in positive electro-spray ionization mode. a) detecting in a LC-MS/MS step, - the reversed phase LC process using as mobile phase, a mixture of acetic acid, acetonitrile and water, the proportion of the acetonitrile solution over the water solution gradually increasing during the process, and at the end diminishing to recondition the LC column, - the mass spectrometer operating in negative electro-spray ionization mode, and - the reversed phase LC process using as mobile phase, a mixture of acetic acid, acetonitrile and water, the proportion of the acetonitrile solution over the water solution gradually increasing during the process, and at the end diminishing to recondition the LC column, - the mass spectrometer operating in negative electro-spray ionization mode, and b) detecting in another LC-MS/MS step, - the reversed phase LC process using as mobile phase, a mixture of ammonium formate, formic acid, water and methanol, the proportion of the methanol solution over the water solution gradually increasing during the process, and at the end diminishing to recondition the LC column, - the mass spectrometer operating in positive electro-spray ionization mode. - the reversed phase LC process using as mobile phase, a mixture of ammonium formate, formic acid, water and methanol, the proportion of the methanol solution over the water solution gradually increasing during the process, and at the end diminishing to recondition the LC column, - the mass spectrometer operating in positive electro-spray ionization mode.