A multipoint sensing method applicable to a capacitive touch panel comprising a first electrode layer and a second electrode layer being electrically insulated and stacked with the first electrode layer, and the first electrode layer or the second electrode layer being adapted to be touched, the multipoint sensing method comprising the steps of: a). inputting an AC signal (Vi) to a plurality of first electrodes of the first electrode layer and to a plurality of second electrodes of the second electrode layer before a touch event to detect a plurality of initial first capacitances (Chc) between each two adjacent ones of the first electrodes, a plurality of initial second capacitances (Chr) between each two adjacent ones of the second electrodes and a plurality of initial vertical capacitances (Cv) of intersection of one of the first electrode and one of the second electrode, wherein the plurality of first electrodes of the first electrode layer is orthogonal to the plurality of second electrodes of the second electrode layer; b). using the AC signal (Vi) to detect variations of each of the initial first capacitance (Chc) and each of the initial second capacitances (Chr), and recording positions of the first electrodes and the second electrodes corresponding to the variations respectively; and c). using the AC signal (Vi) to measure vertical capacitances (Cv') at each of the intersections of the first electrode and the second electrode recorded in step b), and comparing the vertical capacitances (Cv') with the initial vertical capacitances (Cv) measured in step a), and determining the intersections corresponding to the measured vertical capacitances (Cv') to be touch points (a, b, c, d) when the comparison result shows a difference, wherein the multipoint sensing method further comprises a capacitive sensing procedure for measuring one of the initial first capacitances (Chc), the initial second capacitances (Chr), the vertical capacitances (Cv') and the initial vertical capacitances (Cv), the capacitance sensing procedure is to provide a negative feedback amplifier having one of the initial first capacitances (Chc), the initial second capacitances (Chr), the initial vertical capacitances (Cv) and the vertical capacitances (Cv') to be a feedback loop for respectively measuring one of the initial first capacitances (Chc), the initial second capacitances (Chr), the initial vertical capacitances (Cv) and the vertical capacitances (Cv'), the multipoint sensing method further comprising a step of providing an input resistor (Rin) electrically connected with the feedback loop of the negative feedback amplifier for constructing an integrating amplifier with one of the initial first capacitances (Chc) the initial second capacitances (Chr), the vertical capacitances (Cv') and the initial vertical capacitances (Cv). a). inputting an AC signal (Vi) to a plurality of first electrodes of the first electrode layer and to a plurality of second electrodes of the second electrode layer before a touch event to detect a plurality of initial first capacitances (Chc) between each two adjacent ones of the first electrodes, a plurality of initial second capacitances (Chr) between each two adjacent ones of the second electrodes and a plurality of initial vertical capacitances (Cv) of intersection of one of the first electrode and one of the second electrode, wherein the plurality of first electrodes of the first electrode layer is orthogonal to the plurality of second electrodes of the second electrode layer; b). using the AC signal (Vi) to detect variations of each of the initial first capacitance (Chc) and each of the initial second capacitances (Chr), and recording positions of the first electrodes and the second electrodes corresponding to the variations respectively; and c). using the AC signal (Vi) to measure vertical capacitances (Cv') at each of the intersections of the first electrode and the second electrode recorded in step b), and comparing the vertical capacitances (Cv') with the initial vertical capacitances (Cv) measured in step a), and determining the intersections corresponding to the measured vertical capacitances (Cv') to be touch points (a, b, c, d) when the comparison result shows a difference, wherein the multipoint sensing method further comprises a capacitive sensing procedure for measuring one of the initial first capacitances (Chc), the initial second capacitances (Chr), the vertical capacitances (Cv') and the initial vertical capacitances (Cv), the capacitance sensing procedure is to provide a negative feedback amplifier having one of the initial first capacitances (Chc), the initial second capacitances (Chr), the initial vertical capacitances (Cv) and the vertical capacitances (Cv') to be a feedback loop for respectively measuring one of the initial first capacitances (Chc), the initial second capacitances (Chr), the initial vertical capacitances (Cv) and the vertical capacitances (Cv'), the multipoint sensing method further comprising a step of providing an input resistor (Rin) electrically connected with the feedback loop of the negative feedback amplifier for constructing an integrating amplifier with one of the initial first capacitances (Chc) the initial second capacitances (Chr), the vertical capacitances (Cv') and the initial vertical capacitances (Cv).