A light sheet imaging system, comprising: an acoustic trapping arrangement for forming an acoustic trap for acoustically trapping a sample using one or more acoustic beams; an optical trapping arrangement for forming an optical trap for optically trapping a part of the sample using one or more optical beams; an optical system for light sheet imaging the sample; and a sample chamber (SC') for holding a fluid in which the sample is contained or suspended, wherein the optical system comprises illumination optics (BE, AS, CL, SM, RL, O1) for generating a light sheet for illuminating a region through the sample and detection optics (O2, ETL, TL) for imaging light emitted from the illuminated region of the sample, wherein the illumination optics (BE, AS, CL, SM, RL, O1) are configured so that the light sheet propagates along an illumination axis and the detection optics (O2, ETL, TL) are configured to image emitted light propagating along a detection axis, wherein the illumination axis and the detection axis together define an optical system plane, wherein the one or more acoustic beams propagate along an acoustic axis which defines a non-zero angle relative to the optical system plane, wherein the acoustic axis extends through the sample chamber (SC'), wherein the sample chamber (SC') comprises an opening, wherein the illumination optics (BE, AS, CL, SM, RL, O1) and the detection optics (O2, ETL, TL) are located on the opposite side of the sample chamber (SC') to the opening, wherein the sample chamber (SC') comprises, or is at least partially defined by, a wall, wherein the wall is transparent to the light sheet used to illuminate the sample and to the light emitted from the sample, and wherein the wall is configured to transmit the one or more acoustic beams. an acoustic trapping arrangement for forming an acoustic trap for acoustically trapping a sample using one or more acoustic beams; an optical trapping arrangement for forming an optical trap for optically trapping a part of the sample using one or more optical beams; an optical system for light sheet imaging the sample; and a sample chamber (SC') for holding a fluid in which the sample is contained or suspended, wherein the optical system comprises illumination optics (BE, AS, CL, SM, RL, O1) for generating a light sheet for illuminating a region through the sample and detection optics (O2, ETL, TL) for imaging light emitted from the illuminated region of the sample, wherein the illumination optics (BE, AS, CL, SM, RL, O1) are configured so that the light sheet propagates along an illumination axis and the detection optics (O2, ETL, TL) are configured to image emitted light propagating along a detection axis, wherein the illumination axis and the detection axis together define an optical system plane, wherein the one or more acoustic beams propagate along an acoustic axis which defines a non-zero angle relative to the optical system plane, wherein the acoustic axis extends through the sample chamber (SC'), wherein the sample chamber (SC') comprises an opening, wherein the illumination optics (BE, AS, CL, SM, RL, O1) and the detection optics (O2, ETL, TL) are located on the opposite side of the sample chamber (SC') to the opening, wherein the sample chamber (SC') comprises, or is at least partially defined by, a wall, wherein the wall is transparent to the light sheet used to illuminate the sample and to the light emitted from the sample, and wherein the wall is configured to transmit the one or more acoustic beams.