A fluorescence spectrophotometer comprising a light source, an excitation side spectroscope configured to separate light from the light source to generate excitation light and an integrating sphere, which has an inner surface configured to scatter the excitation light that has entered the integrating sphere, comprising a sample holder, which is provided at a position on the integrating sphere that is not directly irradiated with the excitation light that has entered the integrating sphere and that is capable of being irradiated with the excitation light that has been scattered by the inner surface, and which is capable of holding a sample to be measured; a detector configured to detect fluorescent light emitted from the sample irradiated with the excitation light that has been scattered by the inner surface, wherein the fluorescent light emitted from the sample is reflected and scattered by the inner surface of the integrating sphere, and then the fluorescent light exits from the integrating sphere and is guided to the detector; and an imaging device configured to take a sample image of the sample that emits the fluorescent light, characterized in that the integrating sphere comprises six ports (P1 to P6), wherein the first port and the third port, the second port and the fourth port, and the fifth port and the sixth port are formed at positions opposite to each other with a center point of the integrating sphere being interposed therebetween and wherein the excitation light enters the integrating sphere through the first port, the second port is configured to extract the fluorescent light in a direction of 90 degrees with respect to a direction of incidence of the excitation light such that the fluorescent light which passed the second port is detected by the detector, the sample holder is mounted on the fifth port, the imaging device is placed to be opposed to the sixth port and the third port and the fourth port are closed to form a part of the inner surface of the integrating sphere. a sample holder, which is provided at a position on the integrating sphere that is not directly irradiated with the excitation light that has entered the integrating sphere and that is capable of being irradiated with the excitation light that has been scattered by the inner surface, and which is capable of holding a sample to be measured; a detector configured to detect fluorescent light emitted from the sample irradiated with the excitation light that has been scattered by the inner surface, wherein the fluorescent light emitted from the sample is reflected and scattered by the inner surface of the integrating sphere, and then the fluorescent light exits from the integrating sphere and is guided to the detector; and an imaging device configured to take a sample image of the sample that emits the fluorescent light, characterized in that the integrating sphere comprises six ports (P1 to P6), wherein the first port and the third port, the second port and the fourth port, and the fifth port and the sixth port are formed at positions opposite to each other with a center point of the integrating sphere being interposed therebetween and wherein the excitation light enters the integrating sphere through the first port, the second port is configured to extract the fluorescent light in a direction of 90 degrees with respect to a direction of incidence of the excitation light such that the fluorescent light which passed the second port is detected by the detector, the sample holder is mounted on the fifth port, the imaging device is placed to be opposed to the sixth port and the third port and the fourth port are closed to form a part of the inner surface of the integrating sphere.