A fluid transfer device, comprising: a syringe barrel having an inside surface defining a chamber and a distal end portion comprising a passageway; a first plunger slidably movable inside the chamber, the first plunger comprising a proximal end portion, an elongate body portion, and a distal end portion, the distal end portion of the first plunger providing a circumferential seal against the inside surface of the syringe barrel; a second plunger slidably movable inside the chamber, the second plunger comprising a proximal end portion, an elongate body portion, and a distal end portion, the distal end portion of the second plunger providing a circumferential seal against the inside surface of the syringe barrel; wherein the distal end portion of the first plunger is engageable with the proximal end portion of the second plunger such that when the distal end portion of the first plunger and the proximal end portion of the second plunger are engaged, the second plunger is movable by the first plunger; wherein the elongate body portion of the first plunger comprises an internal channel and the distal end portion of the first plunger has a pass-through into the internal channel; wherein the elongate body portion of the second plunger is slidably movable through the pass-through, and the proximal end portion of the second plunger is engageable with the distal end portion of the first plunger; wherein a sealed first volume is defined between the distal end portion of the first plunger and the distal end portion of the second plunger when the first plunger is proximally moved relative to the second plunger, and a second volume is defined between the distal end portion of the second plunger and the distal end portion of the syringe barrel when the second plunger is proximally moved by the first plunger, the elongate body portion of the second plunger having an internal passageway in fluid communication with the sealed first volume, the second plunger further comprising a check valve in the distal end portion. a syringe barrel having an inside surface defining a chamber and a distal end portion comprising a passageway; a first plunger slidably movable inside the chamber, the first plunger comprising a proximal end portion, an elongate body portion, and a distal end portion, the distal end portion of the first plunger providing a circumferential seal against the inside surface of the syringe barrel; a second plunger slidably movable inside the chamber, the second plunger comprising a proximal end portion, an elongate body portion, and a distal end portion, the distal end portion of the second plunger providing a circumferential seal against the inside surface of the syringe barrel; wherein the distal end portion of the first plunger is engageable with the proximal end portion of the second plunger such that when the distal end portion of the first plunger and the proximal end portion of the second plunger are engaged, the second plunger is movable by the first plunger; wherein the elongate body portion of the first plunger comprises an internal channel and the distal end portion of the first plunger has a pass-through into the internal channel; wherein the elongate body portion of the second plunger is slidably movable through the pass-through, and the proximal end portion of the second plunger is engageable with the distal end portion of the first plunger; wherein a sealed first volume is defined between the distal end portion of the first plunger and the distal end portion of the second plunger when the first plunger is proximally moved relative to the second plunger, and a second volume is defined between the distal end portion of the second plunger and the distal end portion of the syringe barrel when the second plunger is proximally moved by the first plunger, the elongate body portion of the second plunger having an internal passageway in fluid communication with the sealed first volume, the second plunger further comprising a check valve in the distal end portion.