An assay cartridge comprising: a) a plurality of chambers; and b) a fluidic network including i) a plurality of fluidic conduits connecting said plurality of chambers; and ii) a multi-port valve comprising: (y) a stator comprising a rotor engagement member, at least one valve inlet and a plurality of valve outlets accessible to one or more fluidic conduits in said fluidic network; and (z) a rotor biased toward said stator by a spring and comprising a sealing member disposed between said rotor and said stator, and a stator engagement member configured to disengage said rotor from said stator when said stator engagement member is in communication with said rotor engagement member, wherein, when engaged, said rotor is rotatable to fluidically connect said at least one valve inlet to one of said plurality of valve outlets through a fluidic connector on the rotor while said sealing member seals the remaining valve outlets characterized in that said stator and rotor engagement members are configured to move said rotor away from said stator thereby disengaging said stator and rotor. a) a plurality of chambers; and b) a fluidic network including i) a plurality of fluidic conduits connecting said plurality of chambers; and ii) a multi-port valve comprising: (y) a stator comprising a rotor engagement member, at least one valve inlet and a plurality of valve outlets accessible to one or more fluidic conduits in said fluidic network; and (z) a rotor biased toward said stator by a spring and comprising a sealing member disposed between said rotor and said stator, and a stator engagement member configured to disengage said rotor from said stator when said stator engagement member is in communication with said rotor engagement member, wherein, when engaged, said rotor is rotatable to fluidically connect said at least one valve inlet to one of said plurality of valve outlets through a fluidic connector on the rotor while said sealing member seals the remaining valve outlets characterized in that said stator and rotor engagement members are configured to move said rotor away from said stator thereby disengaging said stator and rotor. i) a plurality of fluidic conduits connecting said plurality of chambers; and ii) a multi-port valve comprising: (y) a stator comprising a rotor engagement member, at least one valve inlet and a plurality of valve outlets accessible to one or more fluidic conduits in said fluidic network; and (z) a rotor biased toward said stator by a spring and comprising a sealing member disposed between said rotor and said stator, and a stator engagement member configured to disengage said rotor from said stator when said stator engagement member is in communication with said rotor engagement member, (y) a stator comprising a rotor engagement member, at least one valve inlet and a plurality of valve outlets accessible to one or more fluidic conduits in said fluidic network; and (z) a rotor biased toward said stator by a spring and comprising a sealing member disposed between said rotor and said stator, and a stator engagement member configured to disengage said rotor from said stator when said stator engagement member is in communication with said rotor engagement member,