A method for purifying recombinant adeno-associated (rAAV) vector particles said method comprising the steps of: (a) harvesting cells and cell culture supernatant comprising rAAV vector particles to produce a harvest; (b) optionally concentrating said harvest produced in step (a) to produce a concentrated harvest; (c) lysing said harvest produced in step (a) or said concentrated harvest produced in step (b) to produce a lysate; (d) treating the lysate produced in step (c) to reduce contaminating nucleic acid in the lysate thereby producing a nucleic acid reduced lysate; (e) filtering said nucleic acid reduced lysate produced in step (d) to produce a clarified lysate, and optionally diluting said clarified lysate to produce a diluted clarified lysate; (f) subjecting said clarified lysate or diluted clarified lysate produced in step (e) to anion exchange column chromatography or cation exchange column chromatography to produce a column eluate comprised of rAAV vector particles, and optionally concentrating said column eluate to produce a concentrated column eluate; (g) subjecting said column eluate or said concentrated column eluate produced in step (f) to size exclusion column chromatography to produce a second column eluate comprised of rAAV vector particles thereby separating rAAV vector particles from protein impurities, and optionally diluting said second column eluate to produce a diluted second column eluate; (h) subjecting said second column eluate or said diluted second column eluate produced in step (g) to cation exchange column chromatography if in step (f) said clarified lysate or diluted clarified lysate produced in step (e) is subjected to anion exchange chromatography or anion exchange column chromatography if in step (f) said clarified lysate or diluted clarified lysate produced in step (e) is subjected to cation exchange chromatography to produce a third column eluate comprised of rAAV vector particles thereby separating rAAV vector particles from protein or other production impurities, and optionally concentrating said third column eluate to produce a concentrated third column eluate; and (i) filtering said third column eluate or said concentrated third column eluate produced in step (h) thereby producing purified rAAV vector particles. (a) harvesting cells and cell culture supernatant comprising rAAV vector particles to produce a harvest; (b) optionally concentrating said harvest produced in step (a) to produce a concentrated harvest; (c) lysing said harvest produced in step (a) or said concentrated harvest produced in step (b) to produce a lysate; (d) treating the lysate produced in step (c) to reduce contaminating nucleic acid in the lysate thereby producing a nucleic acid reduced lysate; (e) filtering said nucleic acid reduced lysate produced in step (d) to produce a clarified lysate, and optionally diluting said clarified lysate to produce a diluted clarified lysate; (f) subjecting said clarified lysate or diluted clarified lysate produced in step (e) to anion exchange column chromatography or cation exchange column chromatography to produce a column eluate comprised of rAAV vector particles, and optionally concentrating said column eluate to produce a concentrated column eluate; (g) subjecting said column eluate or said concentrated column eluate produced in step (f) to size exclusion column chromatography to produce a second column eluate comprised of rAAV vector particles thereby separating rAAV vector particles from protein impurities, and optionally diluting said second column eluate to produce a diluted second column eluate; (h) subjecting said second column eluate or said diluted second column eluate produced in step (g) to cation exchange column chromatography if in step (f) said clarified lysate or diluted clarified lysate produced in step (e) is subjected to anion exchange chromatography or anion exchange column chromatography if in step (f) said clarified lysate or diluted clarified lysate produced in step (e) is subjected to cation exchange chromatography to produce a third column eluate comprised of rAAV vector particles thereby separating rAAV vector particles from protein or other production impurities, and optionally concentrating said third column eluate to produce a concentrated third column eluate; and (i) filtering said third column eluate or said concentrated third column eluate produced in step (h) thereby producing purified rAAV vector particles.