A method for manufacturing a display substrate, comprising: providing a base substrate formed with a pixel definition layer, wherein the pixel definition layer comprises a plurality of barrier wall structures, the plurality of barrier wall structures comprise a plurality of first barrier wall structures and a plurality of second barrier wall structures, the plurality of barrier wall structures define a plurality of pixel regions arranged in an array on the base substrate, the plurality of pixel regions comprise a plurality of pixel regions arranged along a first direction and a plurality of pixel regions arranged along a second direction; by a solution-applying process, forming a solution layer on the base substrate formed with the pixel definition layer, so that the solution layer comprises a solution formed in the plurality of pixel regions, a solution formed on the plurality of first barrier wall structures and a solution formed on the plurality of second barrier wall structures, wherein the solution formed in the plurality of pixel regions, the solution formed on the plurality of first barrier wall structures and the solution formed on the plurality of second barrier wall structures are the same solution, and the plurality of first barrier wall structures are between the pixel regions arranged along the first direction, the plurality of second barrier wall structures are between the pixel regions arranged along the second direction; and wherein the method for manufacturing a display substrate comprises: performing a drying process on the solution formed in the plurality of pixel regions, the solution formed on the plurality of first barrier wall structures and the solution formed on the plurality of second barrier wall structures, so that the solution in each of the plurality of pixel regions forms a light-emitting layer, characterized in that a distance between any adjacent two of the pixel regions arranged along the first direction is greater than a distance between any adjacent two of the pixel regions arranged along the second direction, and in that a volume of the solution on a single one of the plurality of second barrier wall structures is smaller than a volume of the solution on a single one of the plurality of first barrier wall structures. providing a base substrate formed with a pixel definition layer, wherein the pixel definition layer comprises a plurality of barrier wall structures, the plurality of barrier wall structures comprise a plurality of first barrier wall structures and a plurality of second barrier wall structures, the plurality of barrier wall structures define a plurality of pixel regions arranged in an array on the base substrate, the plurality of pixel regions comprise a plurality of pixel regions arranged along a first direction and a plurality of pixel regions arranged along a second direction; by a solution-applying process, forming a solution layer on the base substrate formed with the pixel definition layer, so that the solution layer comprises a solution formed in the plurality of pixel regions, a solution formed on the plurality of first barrier wall structures and a solution formed on the plurality of second barrier wall structures, wherein the solution formed in the plurality of pixel regions, the solution formed on the plurality of first barrier wall structures and the solution formed on the plurality of second barrier wall structures are the same solution, and the plurality of first barrier wall structures are between the pixel regions arranged along the first direction, the plurality of second barrier wall structures are between the pixel regions arranged along the second direction; and wherein the method for manufacturing a display substrate comprises: performing a drying process on the solution formed in the plurality of pixel regions, the solution formed on the plurality of first barrier wall structures and the solution formed on the plurality of second barrier wall structures, so that the solution in each of the plurality of pixel regions forms a light-emitting layer, characterized in that a distance between any adjacent two of the pixel regions arranged along the first direction is greater than a distance between any adjacent two of the pixel regions arranged along the second direction, and in that a volume of the solution on a single one of the plurality of second barrier wall structures is smaller than a volume of the solution on a single one of the plurality of first barrier wall structures. performing a drying process on the solution formed in the plurality of pixel regions, the solution formed on the plurality of first barrier wall structures and the solution formed on the plurality of second barrier wall structures, so that the solution in each of the plurality of pixel regions forms a light-emitting layer, characterized in that a distance between any adjacent two of the pixel regions arranged along the first direction is greater than a distance between any adjacent two of the pixel regions arranged along the second direction, and in that a volume of the solution on a single one of the plurality of second barrier wall structures is smaller than a volume of the solution on a single one of the plurality of first barrier wall structures.