A method of producing a recombinant protein preparation having a target value of one or more of fucosylated glycans, galactosylated glycans, high mannose glycans, and sialylated glycans, the method comprising: (a) providing a cell genetically engineered to express a recombinant protein; (b) culturing the cell in a culture medium comprising 0.1 mg/L to 10 mg/L putrescine and, optionally, further comprising one or more of lysine, ammonium, DMSO, copper, glucose, a growth factor, a vitamin, a lipid, and a peptone, under conditions in which the cell expresses the recombinant protein; and (c) harvesting a preparation of the recombinant protein produced by the cell that meets a target value, relative to total glycans, of one or more of fucosylated glycans, galactosylated glycans, high mannose glycans, and sialylated glycans, wherein (i) the target value of galactosylated glycans, and/or sialylated glycans is a level at least 10% higher than a level, relative to total glycans, of galactosylated glycans, and/or sialylated glycans in a preparation of the recombinant protein produced by culturing the cell in the medium not comprising 0.1 mg/L to 10 mg/L putrescine; (ii) the target value of high mannose glycans is a level at least 10% lower than a level, relative to total glycans, of high mannose glycans in a preparation of the recombinant protein produced by culturing the cell in the medium not comprising 0.1 mg/L to 10 mg/L putrescine; (iii) the target value of fucosylated glycans is 70% to 100% fucosylated glycans; (iv) the target value of galactosylated glycans is 1% to 95% galactosylated glycans; (v) the target value of high mannose glycans is 0.1% to 20% high mannose glycans; and/or (vi) the target value of sialylated glycans is 0.1% to 90% sialylated glycans; and, optionally, (d) evaluating the level of the one or more of fucosylated glycans, galactosylated glycans, high mannose glycans, and sialylated glycans in the recombinant protein preparation; and/or, (e) formulating the preparation into a drug product. (a) providing a cell genetically engineered to express a recombinant protein; (b) culturing the cell in a culture medium comprising 0.1 mg/L to 10 mg/L putrescine and, optionally, further comprising one or more of lysine, ammonium, DMSO, copper, glucose, a growth factor, a vitamin, a lipid, and a peptone, under conditions in which the cell expresses the recombinant protein; and (c) harvesting a preparation of the recombinant protein produced by the cell that meets a target value, relative to total glycans, of one or more of fucosylated glycans, galactosylated glycans, high mannose glycans, and sialylated glycans, wherein (i) the target value of galactosylated glycans, and/or sialylated glycans is a level at least 10% higher than a level, relative to total glycans, of galactosylated glycans, and/or sialylated glycans in a preparation of the recombinant protein produced by culturing the cell in the medium not comprising 0.1 mg/L to 10 mg/L putrescine; (ii) the target value of high mannose glycans is a level at least 10% lower than a level, relative to total glycans, of high mannose glycans in a preparation of the recombinant protein produced by culturing the cell in the medium not comprising 0.1 mg/L to 10 mg/L putrescine; (iii) the target value of fucosylated glycans is 70% to 100% fucosylated glycans; (iv) the target value of galactosylated glycans is 1% to 95% galactosylated glycans; (v) the target value of high mannose glycans is 0.1% to 20% high mannose glycans; and/or (vi) the target value of sialylated glycans is 0.1% to 90% sialylated glycans; and, optionally, (i) the target value of galactosylated glycans, and/or sialylated glycans is a level at least 10% higher than a level, relative to total glycans, of galactosylated glycans, and/or sialylated glycans in a preparation of the recombinant protein produced by culturing the cell in the medium not comprising 0.1 mg/L to 10 mg/L putrescine; (ii) the target value of high mannose glycans is a level at least 10% lower than a level, relative to total glycans, of high mannose glycans in a preparation of the recombinant protein produced by culturing the cell in the medium not comprising 0.1 mg/L to 10 mg/L putrescine; (iii) the target value of fucosylated glycans is 70% to 100% fucosylated glycans; (iv) the target value of galactosylated glycans is 1% to 95% galactosylated glycans; (v) the target value of high mannose glycans is 0.1% to 20% high mannose glycans; and/or (vi) the target value of sialylated glycans is 0.1% to 90% sialylated glycans; and, optionally, (d) evaluating the level of the one or more of fucosylated glycans, galactosylated glycans, high mannose glycans, and sialylated glycans in the recombinant protein preparation; and/or, (e) formulating the preparation into a drug product.