A method of generating cytotoxic T-lymphocytes (CTLs) that target two or more tumor antigens, comprising the steps of: either nucleofecting a population of DCs from an individual with a plurality of plasmids, wherein each plasmid in the plurality encodes at least one epitope of one or more tumor antigens, thereby producing a population of DCs (APCs) that express and present at least one epitope from two or more tumor antigens; or contacting a population of DCs from an individual with a library of peptides, wherein the peptides overlap in sequence to span part or all of an entire antigen, thereby producing a population of DCs (APCs) that present at least one epitope from two or more different antigens; and contacting peripheral blood mononuclear cells (PBMCs) from the individual with the APCs to produce a population of antigen specific T lymphocytes that are capable of responding to at least one epitope from two or more tumor antigens; and culturing the T-lymphocytes in medium in a vessel that has a gas permeable membrane, wherein the components of the medium comprise IL-7, IL12, IL15 and either IL6 or IL27. either nucleofecting a population of DCs from an individual with a plurality of plasmids, wherein each plasmid in the plurality encodes at least one epitope of one or more tumor antigens, thereby producing a population of DCs (APCs) that express and present at least one epitope from two or more tumor antigens; or contacting a population of DCs from an individual with a library of peptides, wherein the peptides overlap in sequence to span part or all of an entire antigen, thereby producing a population of DCs (APCs) that present at least one epitope from two or more different antigens; and contacting peripheral blood mononuclear cells (PBMCs) from the individual with the APCs to produce a population of antigen specific T lymphocytes that are capable of responding to at least one epitope from two or more tumor antigens; and culturing the T-lymphocytes in medium in a vessel that has a gas permeable membrane, wherein the components of the medium comprise IL-7, IL12, IL15 and either IL6 or IL27. nucleofecting a population of DCs from an individual with a plurality of plasmids, wherein each plasmid in the plurality encodes at least one epitope of one or more tumor antigens, thereby producing a population of DCs (APCs) that express and present at least one epitope from two or more tumor antigens; or contacting a population of DCs from an individual with a library of peptides, wherein the peptides overlap in sequence to span part or all of an entire antigen, thereby producing a population of DCs (APCs) that present at least one epitope from two or more different antigens; and contacting peripheral blood mononuclear cells (PBMCs) from the individual with the APCs to produce a population of antigen specific T lymphocytes that are capable of responding to at least one epitope from two or more tumor antigens; and culturing the T-lymphocytes in medium in a vessel that has a gas permeable membrane, wherein the components of the medium comprise IL-7, IL12, IL15 and either IL6 or IL27. contacting peripheral blood mononuclear cells (PBMCs) from the individual with the APCs to produce a population of antigen specific T lymphocytes that are capable of responding to at least one epitope from two or more tumor antigens; and culturing the T-lymphocytes in medium in a vessel that has a gas permeable membrane, wherein the components of the medium comprise IL-7, IL12, IL15 and either IL6 or IL27.