A bicycle saddle, comprising: a leather cushion; a rail, supporting the leather cushion from below, having a rear end fixed to a rear end of the leather cushion; and an adjusting assembly, being affixed to a bottom surface of the leather cushion, and having a bolt holder, an adjusting bolt, and an adjusting seat, the bolt holder being fixed to a front end of the leather cushion and having a retaining wall and a through hole penetrating the retaining wall, the adjusting bolt having a bolt head and a threaded trunk, the bolt head abutting against the retaining wall of the bolt holder and being rotatably received in the through hole of the bolt holder, the threaded trunk having one end connected to the bolt head, and the adjusting seat having a threaded hole that engages with and receives the threaded trunk of the adjusting bolt; characterized in that the rail has a front end provided with an upward-extending portion and a retaining bay defined by the upward-extending portion; the adjusting seat is fixed to the upward-extending portion of the rail and retained by the retaining bay of the rail; and the adjusting seat has a seat body and an internally-threaded bushing, the seat body abutting against one side of the upward-extending portion of the rail and having an axial hole, the axial hole being communicated with the retaining bay of the rail, the internally-threaded bushing having a bushing head and a bushing trunk connected to the bushing head, the bushing head abutting against a reverse side of the upward-extending portion of the rail, the bushing trunk passing through the retaining bay of the rail and then being fixedly received in the axial hole of the seat body, the internally-threaded bushing having the threaded hole, and the threaded hole passing through the bushing head and the bushing trunk. a leather cushion; a rail, supporting the leather cushion from below, having a rear end fixed to a rear end of the leather cushion; and an adjusting assembly, being affixed to a bottom surface of the leather cushion, and having a bolt holder, an adjusting bolt, and an adjusting seat, the bolt holder being fixed to a front end of the leather cushion and having a retaining wall and a through hole penetrating the retaining wall, the adjusting bolt having a bolt head and a threaded trunk, the bolt head abutting against the retaining wall of the bolt holder and being rotatably received in the through hole of the bolt holder, the threaded trunk having one end connected to the bolt head, and the adjusting seat having a threaded hole that engages with and receives the threaded trunk of the adjusting bolt; characterized in that the rail has a front end provided with an upward-extending portion and a retaining bay defined by the upward-extending portion; the adjusting seat is fixed to the upward-extending portion of the rail and retained by the retaining bay of the rail; and the adjusting seat has a seat body and an internally-threaded bushing, the seat body abutting against one side of the upward-extending portion of the rail and having an axial hole, the axial hole being communicated with the retaining bay of the rail, the internally-threaded bushing having a bushing head and a bushing trunk connected to the bushing head, the bushing head abutting against a reverse side of the upward-extending portion of the rail, the bushing trunk passing through the retaining bay of the rail and then being fixedly received in the axial hole of the seat body, the internally-threaded bushing having the threaded hole, and the threaded hole passing through the bushing head and the bushing trunk.