An analogue of IGF-1 of formula, H-A -1 -A 1 -A 2 -A 3 -A 4 -A 5 -A 6 -A 7 -A 8 -A 9 -A 10 -A 11 -A 12 -A 13 -A 14 -A 15 -A 16 -A 17 -A 18 -A 19 -A 20 -A 21 -A 22 -A 23 -A 24 -A 25 -A 26 -A 27 -A 28 -A 29 -A 30 -A 31 -A 32 -A 33 -A 34 -A 35 -A 36 -A 37 -A 38 -A 39 -A 40 -A 41 -A 42 -A 43 -A 44 -A 45 -A 46 -A 47 -A 48 -A 49 -A 50 -A 51 -A 52 -A 53 -A 54 -A 55 -A 56 -A 57 -A 58 -A 59 -A 60 -A 61 -A 62 -A 63 -A 64 -A 65 -A 66 -A 67 -A 68 -A 69 -A 70 -A 71 -R 1, wherein: A 59 is Asn; A -1 is Met, Ser or deleted; A 1 is Gly or deleted; A 2 is Pro, Lys, or deleted; A 3 is Glu or deleted; A 4 is Thr; A 5 is Leu; A 6 is Cys, hCys, β-Me-Cys, N-Me-Cys, or Pen; A 7 is Gly; A 8 is Ala; A 9 is Glu; A 10 is Leu; A 11 is Val; A 12 is Asp; A 13 is Ala; A 14 is Leu; A 15 is Gln; A 16 is Phe; A 17 is Val; A 18 is Cys, hCys, β-Me-Cys, N-Me-Cys, or Pen; A 19 is Gly; A 20 is Asp; A 21 is Arg; A 22 is Gly; A 23 is Phe; A 24 is Tyr; A 25 is Phe; A 26 is Asn; A 27 is Lys, Arg, or Pro; A 28 is Pro or Lys; A 29 is Thr; A 30 is Gly; A 31 is Tyr; A 32 is Gly; A 33 is Ser; A 34 is Ser; A 35 is Ser; A 36 is Arg; A 37 is Arg; A 38 is Ala; A 39 is Pro; A 40 is Gln; A 41 is Thr; A 42 is Gly; A 43 is Ile; A 44 is Val; A 45 is Asp; A 46 is Glu; A 47 is Cys, hCys, β-Me-Cys, N-Me-Cys, or Pen; A 48 is Cys, hCys, β-Me-Cys, N-Me-Cys, or Pen; A 49 is Phe, Arg, Leu, or Thr; A 50 is Arg or Ser; A 51 is Ser, Aib, Arg, or Thr; A 52 is Cys, hCys, β-Me-Cys, N-Me-Cys, or Pen; A 53 is Asp, Arg, or Ser; A 54 is Leu or A6c; A 55 is Arg or Tyr; A 56 is Arg or Gln; A 57 is Leu; A 58 is Glu or Arg; A 60 is Tyr or Phe; A 61 is Cys, hCys, β-Me-Cys, N-Me-Cys, or Pen; A 62 is Ala or Asn; A 63 is Pro, D-Pro, Thr, or deleted; A 64 is Leu, D-Leu, des-Leu, or deleted; A 65 is Lys, D-Lys, des-Lys, Arg, or deleted; A 66 is Pro, D-Pro, or deleted; A 67 is Ala, D-Ala, Aib, or deleted; A 68 is Lys, D-Lys, Arg, or deleted; A 69 is Ser, D-Ser, Aib, Thr, or deleted; A 70 is Ala, D-Ala, Glu, or deleted; and A 71 is deleted, Asp, Glu, Lys, or Ser; and R 1 is OH or NH 2; provided that the side-chains of residue pairs A 6 and A 48, A 47 and A 52, and A 18 and A 61, each form a disulfide bond; or a pharmaceutically acceptable salt thereof. A 59 is Asn; A -1 is Met, Ser or deleted; A 1 is Gly or deleted; A 2 is Pro, Lys, or deleted; A 3 is Glu or deleted; A 4 is Thr; A 5 is Leu; A 6 is Cys, hCys, β-Me-Cys, N-Me-Cys, or Pen; A 7 is Gly; A 8 is Ala; A 9 is Glu; A 10 is Leu; A 11 is Val; A 12 is Asp; A 13 is Ala; A 14 is Leu; A 15 is Gln; A 16 is Phe; A 17 is Val; A 18 is Cys, hCys, β-Me-Cys, N-Me-Cys, or Pen; A 19 is Gly; A 20 is Asp; A 21 is Arg; A 22 is Gly; A 23 is Phe; A 24 is Tyr; A 25 is Phe; A 26 is Asn; A 27 is Lys, Arg, or Pro; A 28 is Pro or Lys; A 29 is Thr; A 30 is Gly; A 31 is Tyr; A 32 is Gly; A 33 is Ser; A 34 is Ser; A 35 is Ser; A 36 is Arg; A 37 is Arg; A 38 is Ala; A 39 is Pro; A 40 is Gln; A 41 is Thr; A 42 is Gly; A 43 is Ile; A 44 is Val; A 45 is Asp; A 46 is Glu; A 47 is Cys, hCys, β-Me-Cys, N-Me-Cys, or Pen; A 48 is Cys, hCys, β-Me-Cys, N-Me-Cys, or Pen; A 49 is Phe, Arg, Leu, or Thr; A 50 is Arg or Ser; A 51 is Ser, Aib, Arg, or Thr; A 52 is Cys, hCys, β-Me-Cys, N-Me-Cys, or Pen; A 53 is Asp, Arg, or Ser; A 54 is Leu or A6c; A 55 is Arg or Tyr; A 56 is Arg or Gln; A 57 is Leu; A 58 is Glu or Arg; A 60 is Tyr or Phe; A 61 is Cys, hCys, β-Me-Cys, N-Me-Cys, or Pen; A 62 is Ala or Asn; A 63 is Pro, D-Pro, Thr, or deleted; A 64 is Leu, D-Leu, des-Leu, or deleted; A 65 is Lys, D-Lys, des-Lys, Arg, or deleted; A 66 is Pro, D-Pro, or deleted; A 67 is Ala, D-Ala, Aib, or deleted; A 68 is Lys, D-Lys, Arg, or deleted; A 69 is Ser, D-Ser, Aib, Thr, or deleted; A 70 is Ala, D-Ala, Glu, or deleted; and A 71 is deleted, Asp, Glu, Lys, or Ser; and R 1 is OH or NH 2;