A method, comprising (a) providing an amount of a reporter oligonucleotide comprising one or more detectable labels; a first binding member being configured to bind an anchor group of a capture nucleic acid; a second binding member capable of capturing the reporter oligonucleotide, wherein the second binding member is a solid matrix comprising one or more different reporter specific capture oligonucleotides being capable of capturing a reporter oligonucleotide on the second binding member by forming complexes with the reporter oligonucleotide, wherein the different reporter specific capture oligonucleotides are arranged on different locations with respect to the second binding member; an amount of a target nucleic acid capable of forming complexes with the reporter oligonucleotide, wherein at least a part of an interaction site of the reporter oligonucleotide being capable of forming a complex with the target nucleic acid is also capable of forming a complex with a reporter specific capture oligonucleotide, the forming of complexes of the target nucleic acid with the reporter oligonucleotide inhibiting capturing of the reporter oligonucleotide by the second binding member; and an amount of the capture nucleic acid wherein each capture nucleic acid comprises a binding portion specific to a region of the target nucleic acid and the anchor group; (b) forming complexes each comprising a target nucleic acid and a capture nucleic acid; (c) contacting the complexes with the first binding member to bind the complexes to the first binding member and removing unbound material; (d) releasing at least a subset of the amount of target nucleic acid from the first binding member; (e) subjecting the target nucleic acid to amplification; (f) forming complexes of a subset of the amount of reporter oligonucleotide with at least a subset of the amount of amplified target nucleic acid; (g) capturing a remaining subset of the amount of reporter oligonucleotide not in complex with an amplified target nucleic acid on the second binding member; and, (h) determining a value indicative for the presence and/or amount of reporter oligonucleotide captured on the second binding member. (a) providing an amount of a reporter oligonucleotide comprising one or more detectable labels; a first binding member being configured to bind an anchor group of a capture nucleic acid; a second binding member capable of capturing the reporter oligonucleotide, wherein the second binding member is a solid matrix comprising one or more different reporter specific capture oligonucleotides being capable of capturing a reporter oligonucleotide on the second binding member by forming complexes with the reporter oligonucleotide, wherein the different reporter specific capture oligonucleotides are arranged on different locations with respect to the second binding member; an amount of a target nucleic acid capable of forming complexes with the reporter oligonucleotide, wherein at least a part of an interaction site of the reporter oligonucleotide being capable of forming a complex with the target nucleic acid is also capable of forming a complex with a reporter specific capture oligonucleotide, the forming of complexes of the target nucleic acid with the reporter oligonucleotide inhibiting capturing of the reporter oligonucleotide by the second binding member; and an amount of the capture nucleic acid wherein each capture nucleic acid comprises a binding portion specific to a region of the target nucleic acid and the anchor group; (b) forming complexes each comprising a target nucleic acid and a capture nucleic acid; (c) contacting the complexes with the first binding member to bind the complexes to the first binding member and removing unbound material; (d) releasing at least a subset of the amount of target nucleic acid from the first binding member; (e) subjecting the target nucleic acid to amplification; (f) forming complexes of a subset of the amount of reporter oligonucleotide with at least a subset of the amount of amplified target nucleic acid; (g) capturing a remaining subset of the amount of reporter oligonucleotide not in complex with an amplified target nucleic acid on the second binding member; and, (h) determining a value indicative for the presence and/or amount of reporter oligonucleotide captured on the second binding member.