A method for reducing or preventing the cosmetic appearance or prevalence of wrinkles, fine lines, hyperpigmentation, laxity, dry skin, scaling and/or transepidermal water loss in skin of a mammalian animal, comprising administering a cosmetic composition to said animal, wherein said composition is obtained or obtainable by a method consisting of the steps of: a) suspending fish eggs, wherein said eggs are not Salmonidae eggs, in a minimal volume of water; b) inducing synchronized, rapid hatching of said eggs, preferably such that hatching is complete within less than 6 hours for more than 80% of the embryos; c) optionally filtering the hatched eggs to obtain hatching fluid; and d) filtering the hatching fluid of b) or c) to obtain the composition, wherein the step of filtering the hatching fluid consists of the steps of: (i) filtering the hatching fluid using a filter with a pore size of at least 5μm, and collecting the filtrate; (ii) filtering the filtrate from step (i) using a filter with a pore size of 0.30-0.60μm, and collecting the filtrate; (iii) exchanging the water in the filtrate from step (ii) with a pharmaceutically acceptable buffer by performing diafiltration using a filter with an exclusion size of less than 15kDa; (iv) optionally repeating the diafiltration step of step (iii); (v) optionally diluting the solution from step (iii) or (iv) such that the solution has an enzymatic activity of 1000-10000 mU/L, measured by the capacity of the solution to cleave the Factor Xa chromogenic substrate (CH 3 OCO-D-CHA-Gly-Arg-pNA-AcOH); (vi) filtering the solution from step (iii), (iv) or (v) using a filter with a pore size of 0.15-0.30μm, and collecting the filtrate; and (vii) preparing said cosmetic composition from the filtrate from step (vi). a) suspending fish eggs, wherein said eggs are not Salmonidae eggs, in a minimal volume of water; b) inducing synchronized, rapid hatching of said eggs, preferably such that hatching is complete within less than 6 hours for more than 80% of the embryos; c) optionally filtering the hatched eggs to obtain hatching fluid; and d) filtering the hatching fluid of b) or c) to obtain the composition, wherein the step of filtering the hatching fluid consists of the steps of: (i) filtering the hatching fluid using a filter with a pore size of at least 5μm, and collecting the filtrate; (ii) filtering the filtrate from step (i) using a filter with a pore size of 0.30-0.60μm, and collecting the filtrate; (iii) exchanging the water in the filtrate from step (ii) with a pharmaceutically acceptable buffer by performing diafiltration using a filter with an exclusion size of less than 15kDa; (iv) optionally repeating the diafiltration step of step (iii); (v) optionally diluting the solution from step (iii) or (iv) such that the solution has an enzymatic activity of 1000-10000 mU/L, measured by the capacity of the solution to cleave the Factor Xa chromogenic substrate (CH 3 OCO-D-CHA-Gly-Arg-pNA-AcOH); (vi) filtering the solution from step (iii), (iv) or (v) using a filter with a pore size of 0.15-0.30μm, and collecting the filtrate; and (vii) preparing said cosmetic composition from the filtrate from step (vi). (i) filtering the hatching fluid using a filter with a pore size of at least 5μm, and collecting the filtrate; (ii) filtering the filtrate from step (i) using a filter with a pore size of 0.30-0.60μm, and collecting the filtrate; (iii) exchanging the water in the filtrate from step (ii) with a pharmaceutically acceptable buffer by performing diafiltration using a filter with an exclusion size of less than 15kDa; (iv) optionally repeating the diafiltration step of step (iii); (v) optionally diluting the solution from step (iii) or (iv) such that the solution has an enzymatic activity of 1000-10000 mU/L, measured by the capacity of the solution to cleave the Factor Xa chromogenic substrate (CH 3 OCO-D-CHA-Gly-Arg-pNA-AcOH); (vi) filtering the solution from step (iii), (iv) or (v) using a filter with a pore size of 0.15-0.30μm, and collecting the filtrate; and (vii) preparing said cosmetic composition from the filtrate from step (vi).