A bioprocessing cartridge (414, 905, 1200, 1300, 1400, 1600, 1700, 1800) comprising: a) at least one bioprocessing chamber (440, 1310, 1460, 1607, 1803, 1804, 1806, 1808, 1903, 1904, 1907, 1908, 2003, 2004) configured to contain a solid support; b) a plurality of mesoscale and/or microscale process fluid channels (1220, 1410, 1620, 1792, 1892) in fluid communication with the bioprocessing chamber through at least one pump (1445, 1810, 1812, 1814, 1816), wherein the at least one pump is included in or on the bioprocessing cartridge, wherein the term microscale refers to channels having at least one cross-sectional dimension on the order of about 0.1 μm to less than about 1000 μm, and wherein the term mesoscale refers to channels having at least one cross-sectional dimension on the order of about 1000 μm to about 4 mm; c) one or more process fluid connectors comprising aspiration/expiration tubes configured to fluidly connect the bioprocessing cartridge to one or more process fluid reservoirs that are not on the bioprocessing cartridge; and characterized in that the cartridge further comprises d) a slot alignment guide (1340, 1455, 1796, 1896) configured to ensure the cartridge is inserted in the proper orientation into the cartridge slot of a bioprocessing device, such that when inserted into the cartridge slot, the aspiration/expiration tubes extend away from the cartridge and into the process fluid reservoirs, wherein the slot alignment guide consists of one or more tabs, projections, or grooves configured to fit within a corresponding opening in the cartridge slot as the cartridge is inserted into the cartridge slot; e) further characterized in that the or each of the at least one bioprocessing chambers has a fluid volume with or without the solid support of between 4 and 50 mL. a) at least one bioprocessing chamber (440, 1310, 1460, 1607, 1803, 1804, 1806, 1808, 1903, 1904, 1907, 1908, 2003, 2004) configured to contain a solid support; b) a plurality of mesoscale and/or microscale process fluid channels (1220, 1410, 1620, 1792, 1892) in fluid communication with the bioprocessing chamber through at least one pump (1445, 1810, 1812, 1814, 1816), wherein the at least one pump is included in or on the bioprocessing cartridge, wherein the term microscale refers to channels having at least one cross-sectional dimension on the order of about 0.1 μm to less than about 1000 μm, and wherein the term mesoscale refers to channels having at least one cross-sectional dimension on the order of about 1000 μm to about 4 mm; c) one or more process fluid connectors comprising aspiration/expiration tubes configured to fluidly connect the bioprocessing cartridge to one or more process fluid reservoirs that are not on the bioprocessing cartridge; and characterized in that the cartridge further comprises d) a slot alignment guide (1340, 1455, 1796, 1896) configured to ensure the cartridge is inserted in the proper orientation into the cartridge slot of a bioprocessing device, such that when inserted into the cartridge slot, the aspiration/expiration tubes extend away from the cartridge and into the process fluid reservoirs, wherein the slot alignment guide consists of one or more tabs, projections, or grooves configured to fit within a corresponding opening in the cartridge slot as the cartridge is inserted into the cartridge slot; e) further characterized in that the or each of the at least one bioprocessing chambers has a fluid volume with or without the solid support of between 4 and 50 mL.