A method of preparing a composition for regenerating cartilage using lyophilization hyaline cartilage powder, comprising: A) preparing hyaline cartilage; B) preparing lyophilization hyaline cartilage powder by freeze-drying and crushing the hyaline cartilage; C) preparing an adipose tissue extract from an autologous adipose tissue, wherein Step C) comprises C1) removing fibers in autologous adipose tissue using a first filter having a pore diameter of 2 to 3 mm; C2) sequentially filtering the fiber-free autologous adipose tissue through a second filter having a pore diameter of 450 to 550 μm and a third filter having a pore diameter of 150 to 250 μm, and crushing the autologous adipose tissue; and C3) obtaining an adipose tissue extract by filtering the crushed autologous adipose tissue through a fourth filter having a pore diameter of 25 to 75 μm, and then collecting a residue collected by the fourth filter, wherein the concentration of the lyophilization hyaline cartilage powder is 0.005 to 0.07%(w/v), and wherein the lyophilization hyaline cartilage powder has a particle diameter of 30 μm or more and 300 μm or less; and D) preparing a composition for regenerating cartilage which includes the lyophilization hyaline cartilage powder and the adipose tissue extract. A) preparing hyaline cartilage; B) preparing lyophilization hyaline cartilage powder by freeze-drying and crushing the hyaline cartilage; C) preparing an adipose tissue extract from an autologous adipose tissue, wherein Step C) comprises C1) removing fibers in autologous adipose tissue using a first filter having a pore diameter of 2 to 3 mm; C2) sequentially filtering the fiber-free autologous adipose tissue through a second filter having a pore diameter of 450 to 550 μm and a third filter having a pore diameter of 150 to 250 μm, and crushing the autologous adipose tissue; and C3) obtaining an adipose tissue extract by filtering the crushed autologous adipose tissue through a fourth filter having a pore diameter of 25 to 75 μm, and then collecting a residue collected by the fourth filter, wherein the concentration of the lyophilization hyaline cartilage powder is 0.005 to 0.07%(w/v), and wherein the lyophilization hyaline cartilage powder has a particle diameter of 30 μm or more and 300 μm or less; and C1) removing fibers in autologous adipose tissue using a first filter having a pore diameter of 2 to 3 mm; C2) sequentially filtering the fiber-free autologous adipose tissue through a second filter having a pore diameter of 450 to 550 μm and a third filter having a pore diameter of 150 to 250 μm, and crushing the autologous adipose tissue; and C3) obtaining an adipose tissue extract by filtering the crushed autologous adipose tissue through a fourth filter having a pore diameter of 25 to 75 μm, and then collecting a residue collected by the fourth filter, wherein the concentration of the lyophilization hyaline cartilage powder is 0.005 to 0.07%(w/v), and wherein the lyophilization hyaline cartilage powder has a particle diameter of 30 μm or more and 300 μm or less; and D) preparing a composition for regenerating cartilage which includes the lyophilization hyaline cartilage powder and the adipose tissue extract.