A transdermal permeant delivery system for delivery of at least one permeant into a tissue membrane of a subject, comprising: i) a disposable substrate defining a poration area and having an upper substrate surface, a lower substrate surface, a conduit that extends between the upper substrate surface and the lower substrate surface, and at least one channel in fluid communication with the conduit, said channel being formed within in the poration area on the upper substrate surface; ii) a first release liner having a top surface and an opposed bottom surface, wherein at least a portion of the bottom surface of the first release liner is connected to the upper substrate surface; and iii) a patch that is selectively removable from the top surface of the first release liner comprising: a) a backing layer having an upper surface and an opposed lower surface, a first portion of the backing layer being releaseably mounted thereto the top surface of the first release liner in spaced registration with the poration area of the substrate, and a second portion of the backing layer being folded back into a folded position, in which the lower surface of the second portion of the backing layer faces outwardly away from the upper substrate surface of the substrate; and b) a reservoir mounted thereon a portion of the lower surface of the backing layer and configured for releaseably containing at least one permeant. i) a disposable substrate defining a poration area and having an upper substrate surface, a lower substrate surface, a conduit that extends between the upper substrate surface and the lower substrate surface, and at least one channel in fluid communication with the conduit, said channel being formed within in the poration area on the upper substrate surface; ii) a first release liner having a top surface and an opposed bottom surface, wherein at least a portion of the bottom surface of the first release liner is connected to the upper substrate surface; and iii) a patch that is selectively removable from the top surface of the first release liner comprising: a) a backing layer having an upper surface and an opposed lower surface, a first portion of the backing layer being releaseably mounted thereto the top surface of the first release liner in spaced registration with the poration area of the substrate, and a second portion of the backing layer being folded back into a folded position, in which the lower surface of the second portion of the backing layer faces outwardly away from the upper substrate surface of the substrate; and b) a reservoir mounted thereon a portion of the lower surface of the backing layer and configured for releaseably containing at least one permeant. a) a backing layer having an upper surface and an opposed lower surface, a first portion of the backing layer being releaseably mounted thereto the top surface of the first release liner in spaced registration with the poration area of the substrate, and a second portion of the backing layer being folded back into a folded position, in which the lower surface of the second portion of the backing layer faces outwardly away from the upper substrate surface of the substrate; and b) a reservoir mounted thereon a portion of the lower surface of the backing layer and configured for releaseably containing at least one permeant.