A full-automatic immunoblotting analyzer, comprising: a reagent module configured to load a reagent required for testing; a sample feeding module configured to receive a sample and deliver the sample to a specified sampling station; an adding module configured to suck the reagent from the reagent module and deliver the reagent to a testing slit of an incubating module; wherein the adding module is further configured to suck the sample from the sample feeding module, and deliver the sample to the testing slit of the incubating module; a washing module configured to wash the adding module; the incubating module configured to receive the reagent and the sample delivered by the adding module, and mix and incubate the reagent and the sample; wherein the incubating module is provided with the testing slit configured to carry the reagent and the sample; a liquid injection and suction module provided at an edge of the incubating module and configured to inject washing liquid and suck liquid from the incubating module; a determining module provided at the edge of the incubating module and configured to take an image of a substance in the incubating module and analyze the image, and output a result; and a drying module provided at the edge of the incubating module and configured to dry the incubating module; wherein the incubating module comprises a first driving component and a testing component connected to the first driving component, the first driving component comprises a first driver and a main spindle connected to the first driver, the first driver is configured to drive the main spindle to rotate, an angle between the main spindle and a vertical direction is θ and θ is an acute angle, the testing component comprises a disc sleeved on the main spindle and an incubating tray located in the disc, the disc is provided with a through hole allowing the main spindle to extend through, the disc is provided with a thermal insulation pot to accommodate the incubating tray and a heating film connected to the thermal insulation pot, an angle between the incubating tray and a horizontal plane is β and β=θ, the incubating tray is connected to the main spindle and rotates with the main spindle, the incubating tray is provided with the testing slit configured to carry the sample and the reagent. a reagent module configured to load a reagent required for testing; a sample feeding module configured to receive a sample and deliver the sample to a specified sampling station; an adding module configured to suck the reagent from the reagent module and deliver the reagent to a testing slit of an incubating module; wherein the adding module is further configured to suck the sample from the sample feeding module, and deliver the sample to the testing slit of the incubating module; a washing module configured to wash the adding module; the incubating module configured to receive the reagent and the sample delivered by the adding module, and mix and incubate the reagent and the sample; wherein the incubating module is provided with the testing slit configured to carry the reagent and the sample; a liquid injection and suction module provided at an edge of the incubating module and configured to inject washing liquid and suck liquid from the incubating module; a determining module provided at the edge of the incubating module and configured to take an image of a substance in the incubating module and analyze the image, and output a result; and a drying module provided at the edge of the incubating module and configured to dry the incubating module; wherein the incubating module comprises a first driving component and a testing component connected to the first driving component, the first driving component comprises a first driver and a main spindle connected to the first driver, the first driver is configured to drive the main spindle to rotate, an angle between the main spindle and a vertical direction is θ and θ is an acute angle, the testing component comprises a disc sleeved on the main spindle and an incubating tray located in the disc, the disc is provided with a through hole allowing the main spindle to extend through, the disc is provided with a thermal insulation pot to accommodate the incubating tray and a heating film connected to the thermal insulation pot, an angle between the incubating tray and a horizontal plane is β and β=θ, the incubating tray is connected to the main spindle and rotates with the main spindle, the incubating tray is provided with the testing slit configured to carry the sample and the reagent.