Apparatus for depositing a bioactive agent into a tissue mass of a hollow organ, comprising: a catheter (25, 31, 61, 102, 111, 131, 141, 151) having a proximal end and a distal region and a lumen extending therebetween; an elongated member (20, 34, 34', 62, 90, 103, 104, 120, 138, 148, 154) disposed within the lumen of the catheter (25, 31, 61, 102, 111, 131, 141, 151), the elongated member (20, 34, 34', 62, 90, 103, 104, 120, 138, 148, 154) having a proximal end, a tissue-piercing distal end, and a lumen extending therebetween; an actuator (43, 43', 71) operatively coupled to the elongated member (20, 34, 34', 62, 90, 103, 104, 120, 138, 148, 154), wherein the elongated member (20, 34, 34', 62, 90, 103, 104, 120, 138, 148, 154) is slidably disposed within the lumen of the catheter (25, 31, 61, 102, 111, 131, 141, 151) for selectively extending and retracting the tissue-piercing distal end of the elongated member (20, 34, 34', 62, 90, 103, 104, 120, 138, 148, 154) into the tissue mass to form a needle track; means for stabilizing (100, 105, 112, 117, 132, 142) the catheter (25, 31, 61, 102, 111, 131, 141, 151) and elongated member (20, 34, 34', 62, 90, 103, 104, 120, 138, 148, 154) within the hollow organ, characterized by the elongated member (20, 34, 34', 62, 90, 103, 104, 120, 138, 148, 154) being configured to atraumatically deliver the bioactive agent via the lumen of the elongated member (20, 34, 34', 62, 90, 103, 104, 120, 138, 148, 154) into the needle track during retraction of the tissue piercing distal end of the elongated member (20, 34, 34', 62, 90, 103, 104, 120, 138, 148, 154) by holding a column of bioactive agent stationary utilizing a proximal syringe or internal piston while the needle is being retracted. a catheter (25, 31, 61, 102, 111, 131, 141, 151) having a proximal end and a distal region and a lumen extending therebetween; an elongated member (20, 34, 34', 62, 90, 103, 104, 120, 138, 148, 154) disposed within the lumen of the catheter (25, 31, 61, 102, 111, 131, 141, 151), the elongated member (20, 34, 34', 62, 90, 103, 104, 120, 138, 148, 154) having a proximal end, a tissue-piercing distal end, and a lumen extending therebetween; an actuator (43, 43', 71) operatively coupled to the elongated member (20, 34, 34', 62, 90, 103, 104, 120, 138, 148, 154), wherein the elongated member (20, 34, 34', 62, 90, 103, 104, 120, 138, 148, 154) is slidably disposed within the lumen of the catheter (25, 31, 61, 102, 111, 131, 141, 151) for selectively extending and retracting the tissue-piercing distal end of the elongated member (20, 34, 34', 62, 90, 103, 104, 120, 138, 148, 154) into the tissue mass to form a needle track; means for stabilizing (100, 105, 112, 117, 132, 142) the catheter (25, 31, 61, 102, 111, 131, 141, 151) and elongated member (20, 34, 34', 62, 90, 103, 104, 120, 138, 148, 154) within the hollow organ, characterized by the elongated member (20, 34, 34', 62, 90, 103, 104, 120, 138, 148, 154) being configured to atraumatically deliver the bioactive agent via the lumen of the elongated member (20, 34, 34', 62, 90, 103, 104, 120, 138, 148, 154) into the needle track during retraction of the tissue piercing distal end of the elongated member (20, 34, 34', 62, 90, 103, 104, 120, 138, 148, 154) by holding a column of bioactive agent stationary utilizing a proximal syringe or internal piston while the needle is being retracted.