A mixing container assembly which can be used to grow and separate cells or micro carriers from liquid or fluid medium, comprising: a. a container (114, 214, 314, 414, 514, 614) having a sealing mechanism (116, 216, 316, 416, 516, 616) for being sealed both air and liquid tight; b. at least one port through a portion of the container to allow for the introduction of fluid, gas or components; c. a filter (104, 204, 304, 404, 504, 604, 704, 904, 1004, 1104) with a filtration medium; d. a mixing assembly (102, 202, 302, 402, 502, 602, 702, 902, 1002, 1102) that rotates around the filtration medium to allow for suspension of media or agitation thereof, wherein the mixing assembly has vanes (102B, 102C, 302B, 302C) and a magnetic unit (102D, 212, 312, 412, 512, 612, 912, 1012, 1112) in a lower portion thereof that allows for coupling to a stir plate for driving the vanes of the mixing assembly; characterized in that the mixing container assembly is configured to grow and separate cells or micro carriers from liquid or fluid medium in an aseptic environment; the magnetic units (102D, 212, 312, 412, 512, 612, 912, 1012, 1112) are contained in the vanes (102B, 102C, 302B, 302C); the mixing assembly is suspended from above the filtration unit with an integral bearing device (110, 706); a central diptube (106, 506) extends from the top of the container proximate the bottom of the container and proximate from the top of the filter to the bottom of the filter to allow maximum recovery of material that passes through the filter, wherein the bottom of the filter is proximate to a bottom of the container; and a central support (108, 211, 311, 411, 511, 611, 711, 911, 1011, 1111) is provided which serves as an axis of rotation for the vanes and as a support for the integral bearing device. a. a container (114, 214, 314, 414, 514, 614) having a sealing mechanism (116, 216, 316, 416, 516, 616) for being sealed both air and liquid tight; b. at least one port through a portion of the container to allow for the introduction of fluid, gas or components; c. a filter (104, 204, 304, 404, 504, 604, 704, 904, 1004, 1104) with a filtration medium; d. a mixing assembly (102, 202, 302, 402, 502, 602, 702, 902, 1002, 1102) that rotates around the filtration medium to allow for suspension of media or agitation thereof, wherein the mixing assembly has vanes (102B, 102C, 302B, 302C) and a magnetic unit (102D, 212, 312, 412, 512, 612, 912, 1012, 1112) in a lower portion thereof that allows for coupling to a stir plate for driving the vanes of the mixing assembly; characterized in that the mixing container assembly is configured to grow and separate cells or micro carriers from liquid or fluid medium in an aseptic environment; the magnetic units (102D, 212, 312, 412, 512, 612, 912, 1012, 1112) are contained in the vanes (102B, 102C, 302B, 302C); the mixing assembly is suspended from above the filtration unit with an integral bearing device (110, 706); a central diptube (106, 506) extends from the top of the container proximate the bottom of the container and proximate from the top of the filter to the bottom of the filter to allow maximum recovery of material that passes through the filter, wherein the bottom of the filter is proximate to a bottom of the container; and a central support (108, 211, 311, 411, 511, 611, 711, 911, 1011, 1111) is provided which serves as an axis of rotation for the vanes and as a support for the integral bearing device.