A microfluidic device for analyzing a fluidic sample, the device comprising: (a) a tip shaped to receive a volume of the fluidic sample; (b) a vertical inlet comprising a first valve and adjacent to a horizontal flow of the fluidic sample from the sample tip and is located after the tip; (c) a first sample region (112, 203, 304, 409) located after the first valve, wherein the first sample region (112, 203, 304, 409) is in fluidic communication with the tip and comprises at least one transducer configured to detect an energy property of the fluidic sample in order to generate a first sample reading, wherein the first sample reading is indicative of osmolarity of the fluidic sample; (d) a fluid reservoir (105, 504) fluidically connected to the vertical inlet, wherein the fluid reservoir (105, 504) contains a transfer fluid; (e) a second valve (108, 202, 306, 407, 802) that prevents premature fluid flow from the first sample region to a second sample region (103, 204, 301, 406, 807); and (f) the second sample region (103, 204, 301, 406, 807) is in fluidic communication with the first sample region (112, 203, 304, 409) and shaped to receive at least a portion of the fluidic sample, wherein the second sample region (103, 204, 301, 406, 807) comprises a detection substrate (103, 204, 301, 406) configured to detect one or more analytes in the fluidic sample in order to generate a second sample reading, wherein introduction of the transfer fluid from the reservoir (105, 504) to the first sample region (112, 203, 304, 409) moves the at least a portion of the fluidic sample from the first sample region (112, 203,304, 409) to the second sample region (103, 204,301, 406). (a) a tip shaped to receive a volume of the fluidic sample; (b) a vertical inlet comprising a first valve and adjacent to a horizontal flow of the fluidic sample from the sample tip and is located after the tip; (c) a first sample region (112, 203, 304, 409) located after the first valve, wherein the first sample region (112, 203, 304, 409) is in fluidic communication with the tip and comprises at least one transducer configured to detect an energy property of the fluidic sample in order to generate a first sample reading, wherein the first sample reading is indicative of osmolarity of the fluidic sample; (d) a fluid reservoir (105, 504) fluidically connected to the vertical inlet, wherein the fluid reservoir (105, 504) contains a transfer fluid; (e) a second valve (108, 202, 306, 407, 802) that prevents premature fluid flow from the first sample region to a second sample region (103, 204, 301, 406, 807); and (f) the second sample region (103, 204, 301, 406, 807) is in fluidic communication with the first sample region (112, 203, 304, 409) and shaped to receive at least a portion of the fluidic sample, wherein the second sample region (103, 204, 301, 406, 807) comprises a detection substrate (103, 204, 301, 406) configured to detect one or more analytes in the fluidic sample in order to generate a second sample reading, wherein introduction of the transfer fluid from the reservoir (105, 504) to the first sample region (112, 203, 304, 409) moves the at least a portion of the fluidic sample from the first sample region (112, 203,304, 409) to the second sample region (103, 204,301, 406).