Arrangement for light sheet microscopy, comprising - an illumination optical system with an illumination objective for illuminating a sample situated on a sample carrier in a medium, wherein the sample carrier is aligned with respect to a horizontal, plane reference surface, via an illumination beam path with a light sheet, wherein the optical axis of the illumination objective and the light sheet lie in a plane which, with the normal to the reference surface, forms an illumination angle (ß) which is different from zero, - a detection optical system with a detection objective in a detection beam path, the optical axis of which, with the normal to the reference surface, forms a detection angle (δ) which is different from zero, - a separating layer system with at least one layer of a predefined material with a predefined thickness, which separates the medium from the illumination objective and the detection objective, wherein the separating layer system is in contact with the medium with a base surface aligned parallel to the reference surface at least in the region accessible to the illumination objective and the detection objective for illumination or detection respectively, characterized in that - the arrangement comprises a correction lens system with at least one correction lens (10, 11, 12) for reducing aberrations which arise due to the oblique passage of illumination light and/or light to be detected through interfaces of the separating layer system, wherein - the correction lens system is arranged between illumination objective and separating layer system and between detection objective and separating layer system and - the same at least one correction lens is arranged both in the illumination and in the detection beam path. - an illumination optical system with an illumination objective for illuminating a sample situated on a sample carrier in a medium, wherein the sample carrier is aligned with respect to a horizontal, plane reference surface, via an illumination beam path with a light sheet, wherein the optical axis of the illumination objective and the light sheet lie in a plane which, with the normal to the reference surface, forms an illumination angle (ß) which is different from zero, - a detection optical system with a detection objective in a detection beam path, the optical axis of which, with the normal to the reference surface, forms a detection angle (δ) which is different from zero, - a separating layer system with at least one layer of a predefined material with a predefined thickness, which separates the medium from the illumination objective and the detection objective, wherein the separating layer system is in contact with the medium with a base surface aligned parallel to the reference surface at least in the region accessible to the illumination objective and the detection objective for illumination or detection respectively, characterized in that - the arrangement comprises a correction lens system with at least one correction lens (10, 11, 12) for reducing aberrations which arise due to the oblique passage of illumination light and/or light to be detected through interfaces of the separating layer system, wherein - the correction lens system is arranged between illumination objective and separating layer system and between detection objective and separating layer system and - the same at least one correction lens is arranged both in the illumination and in the detection beam path.