A manufacturing method of two-chamber type combined container-syringe, comprising: a dissolving solution filling process of preparing a plurality of dissolving solution-filled cartridges by inserting an end stopper into an inner side of each cartridge which extends along an axis line and has a cylindrical shape, filling a dissolving solution onto the end stopper inside each cartridge, and inserting a middle stopper into a bypass portion positioned on an upper part of the dissolving solution in each cartridge so that the bypass portion is in a communication state; and a vacuum plugging process of eliminating air bubbles from the dissolving solution in the dissolving solution-filled cartridges and sealing the dissolving solution with the respective middle stopper after the dissolving solution filling process, wherein the vacuum plugging process includes a first cooling processing of cooling the inside of a vacuum plugging equipment in which the dissolving solution-filled cartridges are disposed to a cooling temperature at which the dissolving solution is not frozen, a first decompression processing of decreasing pressure inside the vacuum plugging equipment while the vacuum plugging equipment maintains the cooling temperature after the first cooling processing, wherein the vacuum plugging process further comprises: a middle stopper pushing processing of pushing the middle stoppers of the respective cartridges downward using a middle stopper pushing means and bringing the middle stoppers into contact with the dissolving solution so that the dissolving solution fills the space between the middle stoppers and the respective end stoppers in a bubble-free state after the first decompression processing characterized in that the middle stopper pushing means comprises a plurality of pushing rods, each pushing rod being aligned with an upper end opening of a respective cartridge, wherein the pushing rods are vertically movable by oil or air pressure. a dissolving solution filling process of preparing a plurality of dissolving solution-filled cartridges by inserting an end stopper into an inner side of each cartridge which extends along an axis line and has a cylindrical shape, filling a dissolving solution onto the end stopper inside each cartridge, and inserting a middle stopper into a bypass portion positioned on an upper part of the dissolving solution in each cartridge so that the bypass portion is in a communication state; and a vacuum plugging process of eliminating air bubbles from the dissolving solution in the dissolving solution-filled cartridges and sealing the dissolving solution with the respective middle stopper after the dissolving solution filling process, wherein the vacuum plugging process includes a first cooling processing of cooling the inside of a vacuum plugging equipment in which the dissolving solution-filled cartridges are disposed to a cooling temperature at which the dissolving solution is not frozen, a first decompression processing of decreasing pressure inside the vacuum plugging equipment while the vacuum plugging equipment maintains the cooling temperature after the first cooling processing, wherein the vacuum plugging process further comprises: a middle stopper pushing processing of pushing the middle stoppers of the respective cartridges downward using a middle stopper pushing means and bringing the middle stoppers into contact with the dissolving solution so that the dissolving solution fills the space between the middle stoppers and the respective end stoppers in a bubble-free state after the first decompression processing characterized in that the middle stopper pushing means comprises a plurality of pushing rods, each pushing rod being aligned with an upper end opening of a respective cartridge, wherein the pushing rods are vertically movable by oil or air pressure. a middle stopper pushing processing of pushing the middle stoppers of the respective cartridges downward using a middle stopper pushing means and bringing the middle stoppers into contact with the dissolving solution so that the dissolving solution fills the space between the middle stoppers and the respective end stoppers in a bubble-free state after the first decompression processing characterized in that the middle stopper pushing means comprises a plurality of pushing rods, each pushing rod being aligned with an upper end opening of a respective cartridge, wherein the pushing rods are vertically movable by oil or air pressure.