mhurhangee/ep-ind-claims huggingface dataset:
Method for amplification of an RNA target nucleic acid, comprising the steps of a) transfering a liquid sample with a first volume of less than 2 μl comprising one or more living eukaryotic cells into a vessel, b) adding to said vessel a one-step RT-PCR reaction buffer comprising a thermostable DNA polymerase capable of performing a one-step RT-PCR, and dNTPs, with a second volume, whereas said second volume is at least 2x as large as said first volume, b') incubating said sample at a temperature between 37°C and 65°C for 30 seconds to 5 min. c) heating said vessel for at least 20 seconds at at least 90°C, d) amplifying said target in said vessel by means of a polymerase chain reaction with a thermostable DNA polymerase capable of performing a one-step RT-PCR without performance of an intermediate purification step, wherein the ratio of the number of said living cells of step a) versus the liquid volume in which the polymerase chain reaction of step d) is not greater than 2 cells/μl, said ratio is at least 1 cell/25/μl. a) transfering a liquid sample with a first volume of less than 2 μl comprising one or more living eukaryotic cells into a vessel, b) adding to said vessel a one-step RT-PCR reaction buffer comprising a thermostable DNA polymerase capable of performing a one-step RT-PCR, and dNTPs, with a second volume, whereas said second volume is at least 2x as large as said first volume, b') incubating said sample at a temperature between 37°C and 65°C for 30 seconds to 5 min. c) heating said vessel for at least 20 seconds at at least 90°C, d) amplifying said target in said vessel by means of a polymerase chain reaction with a thermostable DNA polymerase capable of performing a one-step RT-PCR without performance of an intermediate purification step,
Use of a steel alloy for well pipes of perforating units for perforation of borehole casings, with the steel alloy comprised, in mass-%, of Carbon 0.15 -0.22 Manganese (Mn) 1.3 -1.8 Silicon (Si) 0.2 -0.4 Nitrogen 0.006 -0.012 Sulfur <0.003 Chromium (Cr) 0.1 -0.3 Molybdenum (Mo) <0.1 Nickel (Ni) <0.1 Vanadium <0.05 Niobium (Nb) 0.01 -0.05 Titanium (Ti) 0.02 -0.04 Boron 0.0015 -0.003 Calcium (Ca) 0.0008 -0.0020 and iron as well as impurities resulting from smelting as remainder, wherein the steel alloy is heated at a heating rate of 1-100 K/s to an austenitizing temperature between 10 to 50 °C above its transformation temperature Ac3, and held at this austenitizing temperature between 0.1 and 10 minutes and austenitized, subsequently quenched at a quenching rate of >50K/s, so as to adjust a martensite content of >95 %, wherein the structure is comprised of >85 % lath martensite, <15 % plate martensite, remainder bainite, and wherein the remainder is formed of lower bainite, wherein the structure is then tempered starting from room temperature between 1 and 25 minutes at temperatures between 280 °C and 700 °C, and finally cooled in air or quenched in water to room temperature, wherein the steel has a tensile strength Rm ranging from 850 MPa to 1,200 MPa at transverse notch impact toughnesses in a range between 190 and 105 J/cm 2 at room temperature, wherein the product of tensile strength and transverse notch impact toughness lies in the strength range between 850 MPA and 1,200 MPa in a range of 141,000 to 165,000 MPa*J/cm 2.
A process for manufacturing a chewing gum sheet without use of a powdered non-stick agent comprising: a. extruding a slab having a tacky surface of heated chewing gum composition, wherein the slab exiting the extruder contains less than or equal to 0.18 weight% non-stick agent based upon the slab weight; b. conveying the slab on a moving conveyor with impingement air flow sufficiently to cool the surface of the slab; c. passing the slab through at least one cooled roller to form a chewing gum sheet with predetermined dimensions. a. extruding a slab having a tacky surface of heated chewing gum composition, wherein the slab exiting the extruder contains less than or equal to 0.18 weight% non-stick agent based upon the slab weight; b. conveying the slab on a moving conveyor with impingement air flow sufficiently to cool the surface of the slab; c. passing the slab through at least one cooled roller to form a chewing gum sheet with predetermined dimensions.
Use of a diesel boiling-range fuel blend for reducing swellable elastomer shrinkage in diesel-boiling range fuel systems, wherein the diesel boiling-range fuel blend comprises: a renewable diesel in an amount of about 20 vol% to about 45 vol%, wherein the renewable diesel meets European EN standard 15940 and is a hydrogenated vegetable oil; a biodiesel in an amount of about 8 vol% to about 10 vol%, wherein the biodiesel is derived from transesterification of vegetable oils or animal fats into alkyl esters of long-chain fatty acids and meets ASTM standard D6751; and a petrodiesel fuel in an amount of at least about 40 vol% to about 60 vol%, wherein the petrodiesel is a fractional distillate of petroleum containing C 8 to C 28 hydrocarbons, has an aromatics content of at least 30 wt%, and meets ASTM standard D975; wherein the diesel boiling-range fuel blend is capable of producing a volume change of at least one swellable elastomer in a diesel-boiling range fuel system within about 1.0% or within about 0.50% compared to a reference volume change of the least one swellable elastomer, said volume change being measured according to ASTM D471; wherein the volume change is a percentage change in volume of the least one swellable elastomer when contacted with: (i) the petrodiesel fuel, and then (ii) the diesel boiling-range fuel blend, wherein the reference volume change corresponds to the volume change experienced by the swellable elastomer when contacted with the petrodiesel fuel; and wherein the diesel boiling-range fuel blend further comprises aromatics in an amount of less than or equal to about 25 wt.%. a renewable diesel in an amount of about 20 vol% to about 45 vol%, wherein the renewable diesel meets European EN standard 15940 and is a hydrogenated vegetable oil; a biodiesel in an amount of about 8 vol% to about 10 vol%, wherein the biodiesel is derived from transesterification of vegetable oils or animal fats into alkyl esters of long-chain fatty acids and meets ASTM standard D6751; and a petrodiesel fuel in an amount of at least about 40 vol% to about 60 vol%, wherein the petrodiesel is a fractional distillate of petroleum containing C 8 to C 28 hydrocarbons, has an aromatics content of at least 30 wt%, and meets ASTM standard D975; wherein the diesel boiling-range fuel blend is capable of producing a volume change of at least one swellable elastomer in a diesel-boiling range fuel system within about 1.0% or within about 0.50% compared to a reference volume change of the least one swellable elastomer, said volume change being measured according to ASTM D471; wherein the volume change is a percentage change in volume of the least one swellable elastomer when contacted with: (i) the petrodiesel fuel, and then (ii) the diesel boiling-range fuel blend, (i) the petrodiesel fuel, and then (ii) the diesel boiling-range fuel blend, wherein the reference volume change corresponds to the volume change experienced by the swellable elastomer when contacted with the petrodiesel fuel; and wherein the diesel boiling-range fuel blend further comprises aromatics in an amount of less than or equal to about 25 wt.%.
Rubber composition based on at least: - one diene elastomer; - one reinforcing filler; - one crosslinking system; - between 1 and 20 phr of an epoxide; - between 1 and 15 phr of an amine-comprising curing agent, wherein the amine-comprising curing agent comprises at least two amine functional groups and is chosen from the group consisting of p-xylylenediamine, 1,3-bis(aminomethyl)cyclohexane, 1,8-diaminooctane, 3,3'-diaminobenzidine and the mixtures of these compounds - one diene elastomer; - one reinforcing filler; - one crosslinking system; - between 1 and 20 phr of an epoxide; - between 1 and 15 phr of an amine-comprising curing agent,
An instrument for determining the volatile content of a sample, while monitoring and controlling the sample temperature, the instrument comprising: a cavity in which a sample for which the volatile content is to be determined can be placed; a first source of electromagnetic radiation for introducing microwaves into said cavity that have frequencies substantially other than infrared frequencies; a second source of electromagnetic radiation for introducing electromagnetic radiation into said cavity at infrared frequencies different from the microwave frequencies introduced by said first source; an analytical balance for measuring the weight of a sample while the sample is in said cavity and on said balance; a temperature sensor capable of measuring and positioned to measure the temperature of a sample in said cavity and on said balance; and a processor in communication with said balance and each of said first and second sources and said temperature sensor for controlling the introduction of said frequencies of microwave energy and infrared frequencies into said cavity in response to said balance and in response to temperatures measured by said temperature sensor to thereby maintain the sample temperature below the temperature at which the sample would burn until the microwaves from said first source and the infrared frequencies from said second source dry the sample sufficiently for said processor to determine the volatile content of the sample based on the weight change of the sample on said balance. a cavity in which a sample for which the volatile content is to be determined can be placed; a first source of electromagnetic radiation for introducing microwaves into said cavity that have frequencies substantially other than infrared frequencies; a second source of electromagnetic radiation for introducing electromagnetic radiation into said cavity at infrared frequencies different from the microwave frequencies introduced by said first source; an analytical balance for measuring the weight of a sample while the sample is in said cavity and on said balance; a temperature sensor capable of measuring and positioned to measure the temperature of a sample in said cavity and on said balance; and a processor in communication with said balance and each of said first and second sources and said temperature sensor for controlling the introduction of said frequencies of microwave energy and infrared frequencies into said cavity in response to said balance and in response to temperatures measured by said temperature sensor to thereby maintain the sample temperature below the temperature at which the sample would burn until the microwaves from said first source and the infrared frequencies from said second source dry the sample sufficiently for said processor to determine the volatile content of the sample based on the weight change of the sample on said balance.
Distributor, in particular sowing machine with a storage container and a metering device which consists of a plurality of spaced-apart metering units and which is arranged on a rotatably driven sowing shaft and has sowing wheels located between two upright walls of a sowing housing and floor flaps which are arranged on a rotatably mounted floor flap shaft and are assigned to the sowing wheels, wherein an actuating lever acts on the floor flap shaft for rotation therewith in order to adjust the distance of the floor flaps with respect to the circumference of the sowing wheels, characterized in that the actuating lever acts at least approximately over half the length of the length of the floor flap shaft, and in that a rotatably mounted adjustment shaft is arranged running parallel to the floor flap shaft, in that, at the one end of the adjustment shaft, an adjustment element preferably in the form of an adjustment lever is arranged laterally on the sowing machine, in that a transmission lever is arranged spaced apart from the adjustment lever on the adjustment shaft, which extends from the actuating lever fastened to the floor flap shaft to laterally outside the sowing machine, and in that the transmission lever is operatively connected to the actuating lever.
An asparaginase which is: a) a polypeptide having an amino acid sequence as shown in any one of SEQ ID NO: 2 or SEQ ID NO: 4; or b) a polypeptide which has at least 90% homology with a polypeptide according to a) and wherein the polypeptide has a width of the pH activity profile which is at least 4 pH units wide, wherein the width of the pH activity profile is the width of the pH range calculated in pH units where the enzyme exhibits 50 to 100% of its maximal activity; or c) a polypeptide which is encoded by a nucleic acid sequence according to any one of SEQ ID NO: 1 or SEQ ID NO: 3 encoding the mature polypeptide. a) a polypeptide having an amino acid sequence as shown in any one of SEQ ID NO: 2 or SEQ ID NO: 4; or b) a polypeptide which has at least 90% homology with a polypeptide according to a) and wherein the polypeptide has a width of the pH activity profile which is at least 4 pH units wide, wherein the width of the pH activity profile is the width of the pH range calculated in pH units where the enzyme exhibits 50 to 100% of its maximal activity; or c) a polypeptide which is encoded by a nucleic acid sequence according to any one of SEQ ID NO: 1 or SEQ ID NO: 3 encoding the mature polypeptide.
A device for preparing a liner to be installed in a pipe, the device comprising a frame, which is rotationally symmetrical in relation to its rotational axis, the frame having two ends, in the middle of both of which ends is arranged an opening, and the device further comprising two rings in connection with the frame, which are equally large in their outer diameter and said outer diameter is larger than the outer diameter of the frame in the section between the rings, wherein each ring of said two rings is fastened to a corresponding end of the frame, the rings being in their outer diameter 5-30 mm larger the outer diameter of the frame in the section between the rings, and the device further comprising a handle for using the device with one hand, wherein said handle comprises two axis parts parallel to the rotational axis of the frame, which are adapted to be inserted into the openings in the ends of the frame; a frame part linking the axis parts; a support part connected to the frame part, which is transverse in relation to the rotational axis of the frame; and an elbow support in connection with the support part, wherein said elbow support of the handle is in its shape a ring or pipe, having an outer diameter and an inner diameter, wherein the inner diameter of the elbow support is, at its narrowest point, at least 50 mm. wherein said handle comprises two axis parts parallel to the rotational axis of the frame, which are adapted to be inserted into the openings in the ends of the frame; a frame part linking the axis parts; a support part connected to the frame part, which is transverse in relation to the rotational axis of the frame; and an elbow support in connection with the support part, wherein said elbow support of the handle is in its shape a ring or pipe, having an outer diameter and an inner diameter, wherein the inner diameter of the elbow support is, at its narrowest point, at least 50 mm.
A drug delivery device for setting and injecting set doses of a drug from a drug-filled reservoir, comprising: - a Gray code type detector for detecting relative movements between a first element and a second element, said elements (230, 250) moving relatively during dose setting and/or during injection wherein the Gray code type detector comprises a code track (231a, 231b) disposed on the first element, the code track (231a, 231b) comprising a sequence of markings alternating between first and second states, and wherein the code track (231a, 231b) is associated with a plurality of detectors (292a, 292b, 293a, 293b, 294a, 294b, 294c) disposed on the second element, each detector (292a, 292b, 293a, 293b, 294a, 294b, 294c) being adapted to sense the states of the markings, characterized in that the sequence of markings consists of alternating first and second areas of respective first and second states, wherein each of the first areas has an extension along the code track of extension X 1 and each of the second areas has an extension along the code track of extension X 2, and wherein the plurality of detectors (292a, 292b, 293a, 293b, 294a, 294b, 294c) are provided as at least three detectors (294a, 294b, 294c) adapted to sense the state of said sequence of markings, the at least three detectors being mutually offset in a direction extending along the code track (231a, 231b) to provide a reading sequence of a Gray code scheme when the at least three detectors (294a, 294b, 294c) are moved relatively to the code track (231a,231b) along the sequence of markings. - a Gray code type detector for detecting relative movements between a first element and a second element, said elements (230, 250) moving relatively during dose setting and/or during injection wherein the Gray code type detector comprises a code track (231a, 231b) disposed on the first element, the code track (231a, 231b) comprising a sequence of markings alternating between first and second states, and wherein the code track (231a, 231b) is associated with a plurality of detectors (292a, 292b, 293a, 293b, 294a, 294b, 294c) disposed on the second element, each detector (292a, 292b, 293a, 293b, 294a, 294b, 294c) being adapted to sense the states of the markings,
A photovoltachromic device comprising a photoelectrode and a counterelectrode, said counterelectrode comprising an electrochromic region and a catalytic region that are physically separate; characterized in that said catalytic region comprises a plurality of first strips and said electrochromic region comprises a plurality of second strips and said electrochromic region and said catalytic region are interdigitated; and wherein said photoelectrode is arranged above said catalytic region; and wherein said photoelectrode comprises a plurality, of third strips, each third strip being arranged opposite a corresponding first strip.
A magnetorheological device comprising: a piston having an axis; a case element surrounding at least a portion of the piston wherein a passage is defined between said piston and said case element; a first magnetic flux generator surrounding a portion of the piston; and a second magnetic flux generator surrounding at least a portion of the first magnetic flux generator; wherein one of said flux generators comprises a permanent magnet to generate a bias flux between the piston and the case element, and the other of said flux generators comprises an electromagnet for providing a controllable magnetic flux between the piston and the case element, wherein the controllable magnetic flux and the bias magnetic flux cooperate to generate a net flux in the passage; wherein at least one of the piston and the case element comprises a material capable of acting in a magnetic circuit to carry a magnetic flux. characterized by the piston includes a first annular magnetic gap portion circumferentially surrounded by, and adjacent, the permanent magnet; an other magnetic gap is between the piston and rod wherein a magnetic gap is a region whose permeability is low compared to that of the other elements of the magnetic circuit; and said first magnetic gap and another magnetic gap each comprise a structural member of aluminum or non-magnetic stainless steel. a piston having an axis; a case element surrounding at least a portion of the piston wherein a passage is defined between said piston and said case element; a first magnetic flux generator surrounding a portion of the piston; and a second magnetic flux generator surrounding at least a portion of the first magnetic flux generator; wherein one of said flux generators comprises a permanent magnet to generate a bias flux between the piston and the case element, and the other of said flux generators comprises an electromagnet for providing a controllable magnetic flux between the piston and the case element, wherein the controllable magnetic flux and the bias magnetic flux cooperate to generate a net flux in the passage; wherein at least one of the piston and the case element comprises a material capable of acting in a magnetic circuit to carry a magnetic flux. characterized by the piston includes a first annular magnetic gap portion circumferentially surrounded by, and adjacent, the permanent magnet; an other magnetic gap is between the piston and rod wherein a magnetic gap is a region whose permeability is low compared to that of the other elements of the magnetic circuit; and said first magnetic gap and another magnetic gap each comprise a structural member of aluminum or non-magnetic stainless steel.
A method for performing a random access procedure to a base station, eNB, at a User Equipment, UE, in a mobile communication system using carrier aggregation in which communication is performed using a plurality of component carriers, the method comprising: transmitting a first random access preamble to the base station through a first uplink component carrier, UL CC, from among a plurality of UL CCs; receiving a first random access response message including a backoff indicator as a response to the first random access preamble through a first downlink component carrier, DL CC, corresponding to the first UL CC; applying a backoff period to the first UL CC in consideration of the backoff indicator received through the first random access response message; and transmitting a second random access preamble to the base station through a second UL CC to which a backoff period is not applied from among the plurality of UL CCs even during the backoff period of the first UL CC. transmitting a first random access preamble to the base station through a first uplink component carrier, UL CC, from among a plurality of UL CCs; receiving a first random access response message including a backoff indicator as a response to the first random access preamble through a first downlink component carrier, DL CC, corresponding to the first UL CC; applying a backoff period to the first UL CC in consideration of the backoff indicator received through the first random access response message; and transmitting a second random access preamble to the base station through a second UL CC to which a backoff period is not applied from among the plurality of UL CCs even during the backoff period of the first UL CC.