A multiple use blood control catheter assembly, comprising: a catheter adapter having a proximal opening, a distal end, and a fluid pathway extending therebetween, the distal end of the catheter adapter housing an intravenous catheter, a portion of the fluid pathway having a first diameter; a septum slidably disposed within the portion of the fluid pathway between a compressed or flexed state and uncompressed or relaxed state, the septum having a second diameter that is at least equal to the first diameter such that the septum divides the fluid pathway into a proximal fluid chamber and a distal fluid chamber, the septum further having a slit providing a pathway through the septum; and a septum actuator having a base positioned within the distal fluid chamber and having a probe configured for insertion within the slit of the septum, wherein, during use of the catheter assembly, the probe biases the slit into an open position when the septum is slid from the uncompressed or relaxed state to the compressed or flexed state in response to an external device being inserted into the proximal opening of the catheter adapter, an external surface of the probe of the septum actuator having a conical shape that causes the septum to be progressively compressed between an outer surface of the probe and an inner surface of the portion of the fluid pathway as the septum is slid towards the compressed or flexed state and the septum actuator is pushed through the slit, the compression of the septum biasing the septum in a proximal direction thereby causing the septum to return to the uncompressed or relaxed state when the external device is withdrawn from the proximal opening, an inner lumen of the probe having a conical shape corresponding to the conical shape of the external surface of the probe. a catheter adapter having a proximal opening, a distal end, and a fluid pathway extending therebetween, the distal end of the catheter adapter housing an intravenous catheter, a portion of the fluid pathway having a first diameter; a septum slidably disposed within the portion of the fluid pathway between a compressed or flexed state and uncompressed or relaxed state, the septum having a second diameter that is at least equal to the first diameter such that the septum divides the fluid pathway into a proximal fluid chamber and a distal fluid chamber, the septum further having a slit providing a pathway through the septum; and a septum actuator having a base positioned within the distal fluid chamber and having a probe configured for insertion within the slit of the septum, wherein, during use of the catheter assembly, the probe biases the slit into an open position when the septum is slid from the uncompressed or relaxed state to the compressed or flexed state in response to an external device being inserted into the proximal opening of the catheter adapter, an external surface of the probe of the septum actuator having a conical shape that causes the septum to be progressively compressed between an outer surface of the probe and an inner surface of the portion of the fluid pathway as the septum is slid towards the compressed or flexed state and the septum actuator is pushed through the slit, the compression of the septum biasing the septum in a proximal direction thereby causing the septum to return to the uncompressed or relaxed state when the external device is withdrawn from the proximal opening, an inner lumen of the probe having a conical shape corresponding to the conical shape of the external surface of the probe.