A tissue suturing device, comprising: a body, comprising a guide segment at a proximal end for extending into a wound to be sutured and a tube segment at a distal end, wherein the guide segment is provided with a suture needle channel, and the tube segment is provided therein with an accommodating cavity communicated with the suture needle channel; a plurality of suture needles, each of which comprising a main needle segment and a puncture needle segment connected with and spaced apart from the main needle segment, wherein two of the suture needles are usable as a group of suture needles to connect a suture thread releasably between the puncture needle segments of the two suture needles, the main needle segments of the plurality of the suture needles can be arranged in the suture needle channel, and the puncture needle segments are configured to carry the suture thread, wherein each of the suture needles can move toward the proximal end and the distal end along the suture needle channel; and characterized in that: the puncture needle segments are configured to be located in the accommodating cavity; wherein each of the suture needles can move toward the proximal end and the distal end along the suture needle channel such that the puncture needle segment can move out of the accommodating cavity in a direction toward the proximal end to pass with the suture thread through a tissue wall, and the puncture needle segment can return into the accommodating cavity alone in a direction toward the distal end after passing with the suture thread through the tissue wall. a body, comprising a guide segment at a proximal end for extending into a wound to be sutured and a tube segment at a distal end, wherein the guide segment is provided with a suture needle channel, and the tube segment is provided therein with an accommodating cavity communicated with the suture needle channel; a plurality of suture needles, each of which comprising a main needle segment and a puncture needle segment connected with and spaced apart from the main needle segment, wherein two of the suture needles are usable as a group of suture needles to connect a suture thread releasably between the puncture needle segments of the two suture needles, the main needle segments of the plurality of the suture needles can be arranged in the suture needle channel, and the puncture needle segments are configured to carry the suture thread, wherein each of the suture needles can move toward the proximal end and the distal end along the suture needle channel; and characterized in that: the puncture needle segments are configured to be located in the accommodating cavity; wherein each of the suture needles can move toward the proximal end and the distal end along the suture needle channel such that the puncture needle segment can move out of the accommodating cavity in a direction toward the proximal end to pass with the suture thread through a tissue wall, and the puncture needle segment can return into the accommodating cavity alone in a direction toward the distal end after passing with the suture thread through the tissue wall. the puncture needle segments are configured to be located in the accommodating cavity; wherein each of the suture needles can move toward the proximal end and the distal end along the suture needle channel such that the puncture needle segment can move out of the accommodating cavity in a direction toward the proximal end to pass with the suture thread through a tissue wall, and the puncture needle segment can return into the accommodating cavity alone in a direction toward the distal end after passing with the suture thread through the tissue wall.