An evaluation device comprising: a cell image extraction unit which is configured to extract an image of neural cells indicating a differentiation state of neural cells which are observation targets on the basis of captured images captured at more than one different times; a marker selection unit which is configured to select a marker which is suitable for evaluating differentiation states of the neural cells and is used for immunostaining to evaluate differentiation states of the neural cells, the marker being according to process of differentiation of the neural cells which are the observation targets and; a storage unit stores, as an experimental result, the image of neural cells extracted by the cell image extraction unit after a cell cultivation of a neural cell whose cell type is the same as the observation targets under standard conditions using a combination of more than one of the markers selected by the marker selection unit is performed; and a state determination unit (160, 180) which is configured to compare an experimental result based on the image of neural cells extracted by the cell image extraction unit with the experimental result stored in the storage unit to determine a differentiation state of the neural cells which are the observation targets after a cell cultivation under non-standard conditions using a combination of a plurality of the markers selected for the neural cells which are the observation targets by the marker selection unit is performed; wherein the combination of the plurality of the markers selected by the marker selection unit includes a facilitated marker of which expression is facilitated with the progress of differentiation induction of the neural cells and a suppressed marker of which expression is suppressed with the progress of differentiation induction of the neural cells, the state determination unit (160, 180) determines the differentiation state of the neural cells based on change of an expression states of the facilitated marker and the suppressed marker, and the information on neural cells under the non-standard conditions is information on neural cells in process of differentiation induction under a condition where a substance which potentially influences the neural cells in process of differentiation induction has been added, and the information on neural cells under the standard conditions is information under a condition where the substance has not been added to the neural cells in process of differentiation. a cell image extraction unit which is configured to extract an image of neural cells indicating a differentiation state of neural cells which are observation targets on the basis of captured images captured at more than one different times; a marker selection unit which is configured to select a marker which is suitable for evaluating differentiation states of the neural cells and is used for immunostaining to evaluate differentiation states of the neural cells, the marker being according to process of differentiation of the neural cells which are the observation targets and; a storage unit stores, as an experimental result, the image of neural cells extracted by the cell image extraction unit after a cell cultivation of a neural cell whose cell type is the same as the observation targets under standard conditions using a combination of more than one of the markers selected by the marker selection unit is performed; and a state determination unit (160, 180) which is configured to compare an experimental result based on the image of neural cells extracted by the cell image extraction unit with the experimental result stored in the storage unit to determine a differentiation state of the neural cells which are the observation targets after a cell cultivation under non-standard conditions using a combination of a plurality of the markers selected for the neural cells which are the observation targets by the marker selection unit is performed; wherein the combination of the plurality of the markers selected by the marker selection unit includes a facilitated marker of which expression is facilitated with the progress of differentiation induction of the neural cells and a suppressed marker of which expression is suppressed with the progress of differentiation induction of the neural cells, the state determination unit (160, 180) determines the differentiation state of the neural cells based on change of an expression states of the facilitated marker and the suppressed marker, and the information on neural cells under the non-standard conditions is information on neural cells in process of differentiation induction under a condition where a substance which potentially influences the neural cells in process of differentiation induction has been added, and the information on neural cells under the standard conditions is information under a condition where the substance has not been added to the neural cells in process of differentiation.