A method of producing one or more polynucleotides of interest for sequencing, comprising: a. obtaining a polynucleotide library comprising a plurality of polynucleotides, wherein each polynucleotide comprises a universal primer region, a sample identification region, an adapter region, and an amplicon region from a single sample, wherein the 3' end of the universal primer region is coupled to the 5' end of the sample identification region, wherein the 3' end of the sample identification region is coupled to the 5' end of the adapter region, and wherein the amplicon region is operatively coupled to the adapter region, wherein the sequence of the universal primer region is substantially identical on each polynucleotide in the plurality of polynucleotides, and wherein the sequence of the sample identification region of each polynucleotide from a first single sample is distinct from the sequence of the sample identification region of the other polynucleotides in the library from one or more samples distinct from the first single sample; and b. amplifying the polynucleotide library with a set of primers to produce the one or more polynucleotides of interest for sequencing, wherein the one or more polynucleotides of interest for sequencing comprises a first sequencing region, a first plate identification region, a universal primer region, a sample identification region, an adapter region, an amplicon region from a single sample, and a second sequencing region, wherein the 3' end of the universal primer region is coupled to the 5' end of the sample identification region, wherein the 3' end of the sample identification region is coupled to the 5' end of the adapter region, wherein the first plate identification region is operatively coupled to the universal primer region, wherein the amplicon region is operatively coupled to the adapter region, wherein the first sequencing region is located at the 5' end of the polynucleotide of interest, and wherein the second sequencing region is located at the 3' end of the polynucleotide of interest. wherein: the sample comprises T cells and the polynucleotide of interest is a DNA or RNA sequence from a T-cell receptor gene; or the sample comprises B cells and the polynucleotide of interest is a DNA or RNA sequence from an immunoglobulin (Ig) gene that encodes an antibody. a. obtaining a polynucleotide library comprising a plurality of polynucleotides, wherein each polynucleotide comprises a universal primer region, a sample identification region, an adapter region, and an amplicon region from a single sample, wherein the 3' end of the universal primer region is coupled to the 5' end of the sample identification region, wherein the 3' end of the sample identification region is coupled to the 5' end of the adapter region, and wherein the amplicon region is operatively coupled to the adapter region, wherein the sequence of the universal primer region is substantially identical on each polynucleotide in the plurality of polynucleotides, and wherein the sequence of the sample identification region of each polynucleotide from a first single sample is distinct from the sequence of the sample identification region of the other polynucleotides in the library from one or more samples distinct from the first single sample; and b. amplifying the polynucleotide library with a set of primers to produce the one or more polynucleotides of interest for sequencing, wherein the one or more polynucleotides of interest for sequencing comprises a first sequencing region, a first plate identification region, a universal primer region, a sample identification region, an adapter region, an amplicon region from a single sample, and a second sequencing region, wherein the 3' end of the universal primer region is coupled to the 5' end of the sample identification region, wherein the 3' end of the sample identification region is coupled to the 5' end of the adapter region, wherein the first plate identification region is operatively coupled to the universal primer region, wherein the amplicon region is operatively coupled to the adapter region, wherein the first sequencing region is located at the 5' end of the polynucleotide of interest, and wherein the second sequencing region is located at the 3' end of the polynucleotide of interest. the sample comprises T cells and the polynucleotide of interest is a DNA or RNA sequence from a T-cell receptor gene; or the sample comprises B cells and the polynucleotide of interest is a DNA or RNA sequence from an immunoglobulin (Ig) gene that encodes an antibody.