A biopsy device for insertion to a target site within a living body, comprising: a first needle including a first longitudinal element extending from a first element proximal end to a first element distal end, wherein the first longitudinal element defines a first lumen extending therethrough from a proximal opening at the first element proximal end to a distal opening at the first element distal end, and wherein the first longitudinal element is sufficiently flexible along a length thereof to be inserted to a target site along a tortuous path within a natural body lumen and configured to pierce tissue; a second needle including a second longitudinal element extending from a second element proximal end to a second element distal end, wherein the second longitudinal element is sized to be slidably received in the first lumen and defines a second lumen extending therewithin to a tissue receiving opening at the second element distal end, and wherein the second longitudinal element is sufficiently flexible to be inserted to a target site along a tortuous path within a natural body lumen and configured to pierce tissue, and a connector; wherein an outer diameter of the second longitudinal element is smaller than an inner diameter of the first lumen by an amount selected to create a fluid receiving annular space therebetween when the second longitudinal element is inserted into the first lumen, characterized in that the second longitudinal element includes a filter at a distal end thereof, wherein the filter comprises a plurality of holes in a distal portion of a wall of the second longitudinal element and wherein the holes of the filter are between 8 μm and 10 μm in diameter, the filter thereby permitting non-targeted fluids received in the second lumen to pass therethrough from the second lumen into the fluid receiving annular space while preventing target tissue received in the second lumen from passing therethrough when suction is applied to the fluid receiving annular space within the first longitudinal element and outside the second longitudinal element via the connector. a first needle including a first longitudinal element extending from a first element proximal end to a first element distal end, wherein the first longitudinal element defines a first lumen extending therethrough from a proximal opening at the first element proximal end to a distal opening at the first element distal end, and wherein the first longitudinal element is sufficiently flexible along a length thereof to be inserted to a target site along a tortuous path within a natural body lumen and configured to pierce tissue; a second needle including a second longitudinal element extending from a second element proximal end to a second element distal end, wherein the second longitudinal element is sized to be slidably received in the first lumen and defines a second lumen extending therewithin to a tissue receiving opening at the second element distal end, and wherein the second longitudinal element is sufficiently flexible to be inserted to a target site along a tortuous path within a natural body lumen and configured to pierce tissue, and a connector; wherein an outer diameter of the second longitudinal element is smaller than an inner diameter of the first lumen by an amount selected to create a fluid receiving annular space therebetween when the second longitudinal element is inserted into the first lumen, characterized in that the second longitudinal element includes a filter at a distal end thereof, wherein the filter comprises a plurality of holes in a distal portion of a wall of the second longitudinal element and wherein the holes of the filter are between 8 μm and 10 μm in diameter, the filter thereby permitting non-targeted fluids received in the second lumen to pass therethrough from the second lumen into the fluid receiving annular space while preventing target tissue received in the second lumen from passing therethrough when suction is applied to the fluid receiving annular space within the first longitudinal element and outside the second longitudinal element via the connector.