A three-dimensional microenvironment structure for controlling cell behavior comprising: a nanofiber membrane; and two or more peptide motifs, including a P1 (first peptide) motif and a P2 (second peptide) motif, wherein the nanofiber membrane comprises one or more of PVDF, PAN, PES, and PLA; wherein the P1 (first peptide) motif controls α5β1 or α6β1 integrins activity; wherein the P2 (second peptide) motif controls integrin coreceptor activity, thereby generating a signal for controlling the cell behavior; wherein the coreceptor is one or more selected from Frizzled receptor, FGF receptor, TGF receptor, and LIF receptor; wherein the P1 (first peptide) motif is selected from RGD (SEQ ID NO: 15), GRGDSP (SEQ ID NO: 16), PHSRN-RGDSP (SEQ ID NO: 17), SPPRRARVT (SEQ ID NO: 18), and KNNQKSEPLIGRKKT (SEQ ID NO: 20) as an α5β1 integrin activation motif; wherein preferably the P1 (first peptide) motif is PHSRN-RGDSP; or wherein the P1 (first peptide) motif is selected from GKNTGDHFVLYM (SEQ ID NO: 22), WSLYNFEQTFML (SEQ ID NO: 23), RFDQELRLVSYN (SEQ ID NO: 24), RLVSYSGVLFFLK (SEQ ID NO: 25), ASKAIQVFLLGG (SEQ ID NO: 26), VLVRVERATVFS (SEQ ID NO: 27), TVFSVDQDNMLE (SEQ ID NO: 28), RLRGPQRVFDLH (SEQ ID NO: 29), FDLHQNMGSVN (SEQ ID NO: 30), QQNLGSVNVSTG (SEQ ID NO: 31), and SRATAQKVSRRS (SEQ ID NO: 32) as an α6β1 integrin activation motif; wherein preferably the P1 (first peptide) motif is WSLYNFEQTFML; wherein the P2 motif (second motif) is selected from LCCGRGHRTRTQRVTERCNC and LGTQGRLCNKTSEGMDGCEL as a Frizzled receptor activation motif; or wherein the P2 motif is selected from FLPMSAKS, HFKDPKRLYCK, ANRYLAMKEDGRLLAS, KTGPGQKA, and WYVALKRTGQYKLG as a FGF receptor activation motif; or wherein the P2 motif is selected from MHRMPSFLPTTL and LTGKNFPMFHRN as a TGF receptor activation motif; or wherein the P2 motif is selected from IVPLLLLVLH and YTAQGEPFPNNVEKLCAP as a LIF receptor activation motif; and wherein the integrin activity and integrin coreceptor activity are simultaneously controlled. wherein the nanofiber membrane comprises one or more of PVDF, PAN, PES, and PLA; wherein the P1 (first peptide) motif controls α5β1 or α6β1 integrins activity; wherein the P2 (second peptide) motif controls integrin coreceptor activity, thereby generating a signal for controlling the cell behavior; wherein the coreceptor is one or more selected from Frizzled receptor, FGF receptor, TGF receptor, and LIF receptor; wherein the P1 (first peptide) motif is selected from RGD (SEQ ID NO: 15), GRGDSP (SEQ ID NO: 16), PHSRN-RGDSP (SEQ ID NO: 17), SPPRRARVT (SEQ ID NO: 18), and KNNQKSEPLIGRKKT (SEQ ID NO: 20) as an α5β1 integrin activation motif; wherein preferably the P1 (first peptide) motif is PHSRN-RGDSP; or wherein the P1 (first peptide) motif is selected from GKNTGDHFVLYM (SEQ ID NO: 22), WSLYNFEQTFML (SEQ ID NO: 23), RFDQELRLVSYN (SEQ ID NO: 24), RLVSYSGVLFFLK (SEQ ID NO: 25), ASKAIQVFLLGG (SEQ ID NO: 26), VLVRVERATVFS (SEQ ID NO: 27), TVFSVDQDNMLE (SEQ ID NO: 28), RLRGPQRVFDLH (SEQ ID NO: 29), FDLHQNMGSVN (SEQ ID NO: 30), QQNLGSVNVSTG (SEQ ID NO: 31), and SRATAQKVSRRS (SEQ ID NO: 32) as an α6β1 integrin activation motif; wherein preferably the P1 (first peptide) motif is WSLYNFEQTFML; wherein the P2 motif (second motif) is selected from LCCGRGHRTRTQRVTERCNC and LGTQGRLCNKTSEGMDGCEL as a Frizzled receptor activation motif; or wherein the P2 motif is selected from FLPMSAKS, HFKDPKRLYCK, ANRYLAMKEDGRLLAS, KTGPGQKA, and WYVALKRTGQYKLG as a FGF receptor activation motif; or wherein the P2 motif is selected from MHRMPSFLPTTL and LTGKNFPMFHRN as a TGF receptor activation motif; or wherein the P2 motif is selected from IVPLLLLVLH and YTAQGEPFPNNVEKLCAP as a LIF receptor activation motif; and wherein the integrin activity and integrin coreceptor activity are simultaneously controlled.