A point-of-care testing (POCT) fully-automatic chemiluminescence device based on a multi-channel parallel pretreatment technology, wherein the point-of-care testing fully-automatic chemiluminescence device comprises a support, a reaction chamber assembly, reagent strips, a multi-channel parallel pretreatment assembly and a photomultiplier tube, PMT, assembly, wherein the reaction chamber assembly is provided on the support, and the reaction chamber assembly is configured to accommodate the reagent strips and reciprocates linearly relative to the support; the multi-channel parallel pretreatment assembly and the PMT assembly are sequentially arranged on the support from front to back and are located above the reaction chamber assembly; the multi-channel parallel pretreatment assembly is configured to transfer, clean, and separate reagents in the reagent strips in the reaction chamber assembly; the reaction chamber assembly is configured to transfer the cleaned and separated reagents to the PMT assembly; and the PMT assembly is configured to detect a luminescence value of the cleaned and separated reagents; the reaction chamber assembly comprises a guide rail provided at a bottom of the support; two sides of the guide rail are respectively provided with a reaction chamber stepping motor and a base, and the reaction chamber stepping motor and the base are fixedly connected to the support; an output end of the reaction chamber stepping motor is provided with a reaction chamber driving pulley, and the base is provided with a reaction chamber driven pulley; and the reaction chamber driving pulley is connected to the reaction chamber driven pulley through a belt; a reaction chamber body is provided on the guide rail; a bottom of the reaction chamber body is provided with a reaction chamber slider that is connected to the guide rail in a slidable manner; a side wall of the reaction chamber body is provided with a first synchronous belt pressing plate configured to be in contact with the belt; and the belt drives the reaction chamber body to slide in a length direction of the guide rail through the first synchronous belt pressing plate; and the reagent strips each are provided with a handle; a tip head, a disposable magnetic separation sleeve, and a disposable film breaking piece are arranged beside the handle; and the reagent strips each are provided with a first diluent hole, a second diluent hole, a magnetic bead hole, a first reagent hole, a second reagent hole, a third reagent hole, a first cleaning solution hole, a second cleaning solution hole, a third cleaning solution hole, a fourth cleaning solution hole, and a substrate reading hole side by side. the multi-channel parallel pretreatment assembly and the PMT assembly are sequentially arranged on the support from front to back and are located above the reaction chamber assembly; the multi-channel parallel pretreatment assembly is configured to transfer, clean, and separate reagents in the reagent strips in the reaction chamber assembly; the reaction chamber assembly is configured to transfer the cleaned and separated reagents to the PMT assembly; and the PMT assembly is configured to detect a luminescence value of the cleaned and separated reagents; the reaction chamber assembly comprises a guide rail provided at a bottom of the support; two sides of the guide rail are respectively provided with a reaction chamber stepping motor and a base, and the reaction chamber stepping motor and the base are fixedly connected to the support; an output end of the reaction chamber stepping motor is provided with a reaction chamber driving pulley, and the base is provided with a reaction chamber driven pulley; and the reaction chamber driving pulley is connected to the reaction chamber driven pulley through a belt; a reaction chamber body is provided on the guide rail; a bottom of the reaction chamber body is provided with a reaction chamber slider that is connected to the guide rail in a slidable manner; a side wall of the reaction chamber body is provided with a first synchronous belt pressing plate configured to be in contact with the belt; and the belt drives the reaction chamber body to slide in a length direction of the guide rail through the first synchronous belt pressing plate; and the reagent strips each are provided with a handle; a tip head, a disposable magnetic separation sleeve, and a disposable film breaking piece are arranged beside the handle; and the reagent strips each are provided with a first diluent hole, a second diluent hole, a magnetic bead hole, a first reagent hole, a second reagent hole, a third reagent hole, a first cleaning solution hole, a second cleaning solution hole, a third cleaning solution hole, a fourth cleaning solution hole, and a substrate reading hole side by side.