A method of detecting genetic material in a biological sample including the following stages: a) the biological sample is loaded into a reaction cartridge and then or before that the reaction cartridge is placed in a measurement device, the measurement device comprising a measurement chamber having a receptacle housing the reaction cartridge, wherein the reaction cartridge comprises an isolation chamber for isolating genetic material, which is connected with reaction chambers (8.1, 8.2, 8.3) through channels, for amplifying isolated genetic material, b) the collected biological sample is taken to the isolation chamber, c) biological material is isolated from the tested sample by heating the isolation chamber, d) the isolated genetic material is moved into a plurality of reaction chambers (8.1, 8.2, 8.3), and e) genetic material is amplified by heating the reaction chambers (8.1, 8.2, 8.3), characterized in that: inside at least one of the reaction chambers (8.1, 8.2, 8.3) are present freeze-dried reagents for amplification of genetic material together with luminescent dye, comprising fluorescence dye or quantum-dots binding genetic material to be detected, wherein simultaneously with the stage of amplification of genetic material a detection of luminescent signal from luminescent markers is registered, the measurement device comprises a plurality of heating units of LEDs with temperature detectors arranged substantially opposite the isolation chamber and/or reaction chambers (8.1, 8.2, 8.3) such that light beams emitted by said plurality of LEDs illuminate said isolation chamber and/or reaction chambers (8.1, 8.2, 8.3), and heating of the isolation chamber and/or reaction chamber (8.1, 8.2, 8.3) is performed through the plurality of heating units of LEDs with temperature detectors, and in the isolation chamber is present material capable of binding the inhibitors of amplification reaction. a) the biological sample is loaded into a reaction cartridge and then or before that the reaction cartridge is placed in a measurement device, the measurement device comprising a measurement chamber having a receptacle housing the reaction cartridge, wherein the reaction cartridge comprises an isolation chamber for isolating genetic material, which is connected with reaction chambers (8.1, 8.2, 8.3) through channels, for amplifying isolated genetic material, b) the collected biological sample is taken to the isolation chamber, c) biological material is isolated from the tested sample by heating the isolation chamber, d) the isolated genetic material is moved into a plurality of reaction chambers (8.1, 8.2, 8.3), and e) genetic material is amplified by heating the reaction chambers (8.1, 8.2, 8.3), characterized in that: inside at least one of the reaction chambers (8.1, 8.2, 8.3) are present freeze-dried reagents for amplification of genetic material together with luminescent dye, comprising fluorescence dye or quantum-dots binding genetic material to be detected, wherein simultaneously with the stage of amplification of genetic material a detection of luminescent signal from luminescent markers is registered, the measurement device comprises a plurality of heating units of LEDs with temperature detectors arranged substantially opposite the isolation chamber and/or reaction chambers (8.1, 8.2, 8.3) such that light beams emitted by said plurality of LEDs illuminate said isolation chamber and/or reaction chambers (8.1, 8.2, 8.3), and heating of the isolation chamber and/or reaction chamber (8.1, 8.2, 8.3) is performed through the plurality of heating units of LEDs with temperature detectors, and in the isolation chamber is present material capable of binding the inhibitors of amplification reaction. inside at least one of the reaction chambers (8.1, 8.2, 8.3) are present freeze-dried reagents for amplification of genetic material together with luminescent dye, comprising fluorescence dye or quantum-dots binding genetic material to be detected, wherein simultaneously with the stage of amplification of genetic material a detection of luminescent signal from luminescent markers is registered, the measurement device comprises a plurality of heating units of LEDs with temperature detectors arranged substantially opposite the isolation chamber and/or reaction chambers (8.1, 8.2, 8.3) such that light beams emitted by said plurality of LEDs illuminate said isolation chamber and/or reaction chambers (8.1, 8.2, 8.3), and heating of the isolation chamber and/or reaction chamber (8.1, 8.2, 8.3) is performed through the plurality of heating units of LEDs with temperature detectors, and in the isolation chamber is present material capable of binding the inhibitors of amplification reaction.