A gene sequencing chip, comprising: - an upper substrate including a plurality of liquid inlets for inletting liquid drops, - a lower substrate opposite to the upper substrate and spaced therefrom by a gap, the gap being provided for allowing the liquid drops to move therein, the lower substrate comprising a liquid drop operation region, the liquid drop operation region comprising a manipulation electrode array, wherein the manipulation electrode array comprises: - multiple first sub-arrays for preparing a gene library, and - multiple second sub-arrays for sequencing the prepared gene library, each of the multiple first sub-arrays being adjacent to one of the second sub-arrays, wherein the lower substrate further comprises: - a lower dielectric layer overlaying the liquid drop operation region, and - a lower hydrophobic layer arranged on a surface of the lower dielectric layer closer to the upper substrate, wherein the upper substrate further comprises: - an upper glass substrate, - an ITO reference electrode arranged on a surface of the upper glass substrate closer to the lower substrate, - an upper dielectric layer arranged on a surface of the ITO reference electrode closer to the lower substrate, and - an upper hydrophobic layer arranged on a surface of the upper dielectric layer closer to the lower substrate, wherein the liquid drops comprise raw material liquid drops, gene library preparation reagent liquid drops and sequencing reagent liquid drops, and the plurality of liquid inlets comprise: - an inlet port via which the raw material liquid drops are provided to the first sub-arrays, - a first inlet via which the gene library preparation reagent liquid drops are provided to the first sub-arrays, - a second inlet via which gene amplification reagent liquid drops are provided to the second sub-arrays, - a third inlet via which the sequencing reagent liquid drops are provided to the second sub-arrays, and - an outlet port for discharging a waste liquid, wherein the inlet port, the first inlet, the second inlet, the third inlet and the outlet port all penetrate the upper substrate, the inlet port, the first inlet, the second inlet and the third inlet are all formed in the upper substrate by through hole processing, wherein the manipulation electrode array comprises manipulation electrodes corresponding to the inlet port, the first inlet, the second inlet, the third inlet or the outlet port and configured for controlling an inlet amount or outlet amount of liquid drops at the inlet port, the first inlet, the second inlet, the third inlet or the outlet port, wherein the first sub-array further comprises a rectangular ring-shaped array for mixing the raw material liquid drops and the gene library preparation reagent liquid drop to prepare a gene library, wherein the second sub-array comprises a sequencing channel where the gene sequencing is performed. - an upper substrate including a plurality of liquid inlets for inletting liquid drops, - a lower substrate opposite to the upper substrate and spaced therefrom by a gap, the gap being provided for allowing the liquid drops to move therein, the lower substrate comprising a liquid drop operation region, the liquid drop operation region comprising a manipulation electrode array, wherein the manipulation electrode array comprises: - multiple first sub-arrays for preparing a gene library, and - multiple second sub-arrays for sequencing the prepared gene library, each of the multiple first sub-arrays being adjacent to one of the second sub-arrays, - multiple first sub-arrays for preparing a gene library, and - multiple second sub-arrays for sequencing the prepared gene library, each of the multiple first sub-arrays being adjacent to one of the second sub-arrays, - a lower dielectric layer overlaying the liquid drop operation region, and - a lower hydrophobic layer arranged on a surface of the lower dielectric layer closer to the upper substrate, - an upper glass substrate, - an ITO reference electrode arranged on a surface of the upper glass substrate closer to the lower substrate, - an upper dielectric layer arranged on a surface of the ITO reference electrode closer to the lower substrate, and - an upper hydrophobic layer arranged on a surface of the upper dielectric layer closer to the lower substrate, - an inlet port via which the raw material liquid drops are provided to the first sub-arrays, - a first inlet via which the gene library preparation reagent liquid drops are provided to the first sub-arrays, - a second inlet via which gene amplification reagent liquid drops are provided to the second sub-arrays, - a third inlet via which the sequencing reagent liquid drops are provided to the second sub-arrays, and - an outlet port for discharging a waste liquid, wherein the inlet port, the first inlet, the second inlet, the third inlet and the outlet port all penetrate the upper substrate, the inlet port, the first inlet, the second inlet and the third inlet are all formed in the upper substrate by through hole processing, wherein the manipulation electrode array comprises manipulation electrodes corresponding to the inlet port, the first inlet, the second inlet, the third inlet or the outlet port and configured for controlling an inlet amount or outlet amount of liquid drops at the inlet port, the first inlet, the second inlet, the third inlet or the outlet port, wherein the first sub-array further comprises a rectangular ring-shaped array for mixing the raw material liquid drops and the gene library preparation reagent liquid drop to prepare a gene library, wherein the second sub-array comprises a sequencing channel where the gene sequencing is performed.