A biosensing device for detecting and quantifying a selected peptide or protein of interest present in a biological sample characterized by comprising a cellulose paper test-strip platform comprising an amine-layer, and a biorecognition layer for said POI which is built by molecular imprinting polymer technology and is capable of generating a change of colour after being stained with an adequate peptide staining solution; wherein said cellulose paper test-strip platform is a qualitative filter type paper; wherein said amine layer is obtainable by treating the cellulose paper test-strip platform with a compound selected from a list consisting of: 3-aminopropyltriethoxysilane (APTES), ethylenediamine, phenylenediamine, poly(diallyldimethyl ammonium chloride, poly(vinylamine) hydrochloride, poly(L-lysine hydrobromide), polyethyleneimine hydrochloride, poly(allylamine hydrochloride), poly(4-aminostyrene), poly(allylamine), polyethylene glycol)bis(2-aminoethyl), chitosan, poly(I-lysine hydrobromide); wherein said amine-layer is further incubated with a solution comprising a dialdehyde compound capable of providing two terminal aldehyde functional groups, thus forming an amine-aldehyde layer, wherein a molecularly imprinted polymer layer able to recognize said POI is built on top of said amine-aldehyde layer; and wherein said POI is selected from a list consisting of: peptide Aβ-42, cardiac myoglobin, troponin T, CK-MB isoenzyme, cancer MUC-1 protein, carcinoembryonic antigen and carbohydrate antigen (CA 19-9), neurodegenerative biomarkers such as amyloid β42, Tau protein, interleukin, antibodies such as SARS-CoV-2 (2019-nCoV), antibodies for malaria, zika and dengue virus and respective antigens, viral proteins SARS-CoV-2 (2019-nCoV) antigen), bacterial proteins such as protein A, and serum human albumin. wherein said cellulose paper test-strip platform is a qualitative filter type paper; wherein said amine layer is obtainable by treating the cellulose paper test-strip platform with a compound selected from a list consisting of: 3-aminopropyltriethoxysilane (APTES), ethylenediamine, phenylenediamine, poly(diallyldimethyl ammonium chloride, poly(vinylamine) hydrochloride, poly(L-lysine hydrobromide), polyethyleneimine hydrochloride, poly(allylamine hydrochloride), poly(4-aminostyrene), poly(allylamine), polyethylene glycol)bis(2-aminoethyl), chitosan, poly(I-lysine hydrobromide); wherein said amine-layer is further incubated with a solution comprising a dialdehyde compound capable of providing two terminal aldehyde functional groups, thus forming an amine-aldehyde layer, wherein a molecularly imprinted polymer layer able to recognize said POI is built on top of said amine-aldehyde layer; and wherein said POI is selected from a list consisting of: peptide Aβ-42, cardiac myoglobin, troponin T, CK-MB isoenzyme, cancer MUC-1 protein, carcinoembryonic antigen and carbohydrate antigen (CA 19-9), neurodegenerative biomarkers such as amyloid β42, Tau protein, interleukin, antibodies such as SARS-CoV-2 (2019-nCoV), antibodies for malaria, zika and dengue virus and respective antigens, viral proteins SARS-CoV-2 (2019-nCoV) antigen), bacterial proteins such as protein A, and serum human albumin.