A method for determining a lectin-binding glycan indicative to traumatic brain injury in a fluid sample, the method comprising a) providing i. a probe comprising a porous matrix, the porous matrix comprising ∘ a first part, a second part and a detection zone therebetween and ∘ a first lectin adapted to bind the lectin-binding glycan and being immobilized to the detection zone, and ii. a conjugate comprising ∘ a second lectin adapted to bind the lectin-binding glycan and ∘ a signal generation means adapted to produce a detectable signal upon binding of the second lectin of the conjugate to the lectin-binding glycan immobilized to the detection zone via the first lectin, wherein the first lectin and the second lectin are same lectins and are selected from a group consisting of Urtica dioica, Allium savitum and Phytohemagglutinin-L (PHA-L), b) exposing the detection zone to the fluid sample, c) exposing the detection zone to the conjugate, d) detecting signal derived from the signal generation means at the detection zone, e) determining level of the lectin-binding glycan in the fluid sample based on the detecting, and f) comparing the level of the lectin-binding glycan in the fluid sample to a control level indicative to a level of the lectin-binding glycan in a body fluid of a subject not suffering from TBI, wherein said lectin-binding glucan is indicative to TBI when its level in the fluid sample is at least two times higher than the control level. a) providing i. a probe comprising a porous matrix, the porous matrix comprising ∘ a first part, a second part and a detection zone therebetween and ∘ a first lectin adapted to bind the lectin-binding glycan and being immobilized to the detection zone, and ii. a conjugate comprising ∘ a second lectin adapted to bind the lectin-binding glycan and ∘ a signal generation means adapted to produce a detectable signal upon binding of the second lectin of the conjugate to the lectin-binding glycan immobilized to the detection zone via the first lectin, wherein the first lectin and the second lectin are same lectins and are selected from a group consisting of Urtica dioica, Allium savitum and Phytohemagglutinin-L (PHA-L), i. a probe comprising a porous matrix, the porous matrix comprising ∘ a first part, a second part and a detection zone therebetween and ∘ a first lectin adapted to bind the lectin-binding glycan and being immobilized to the detection zone, and ∘ a first part, a second part and a detection zone therebetween and ∘ a first lectin adapted to bind the lectin-binding glycan and being immobilized to the detection zone, and ii. a conjugate comprising ∘ a second lectin adapted to bind the lectin-binding glycan and ∘ a signal generation means adapted to produce a detectable signal upon binding of the second lectin of the conjugate to the lectin-binding glycan immobilized to the detection zone via the first lectin, ∘ a second lectin adapted to bind the lectin-binding glycan and ∘ a signal generation means adapted to produce a detectable signal upon binding of the second lectin of the conjugate to the lectin-binding glycan immobilized to the detection zone via the first lectin, b) exposing the detection zone to the fluid sample, c) exposing the detection zone to the conjugate, d) detecting signal derived from the signal generation means at the detection zone, e) determining level of the lectin-binding glycan in the fluid sample based on the detecting, and f) comparing the level of the lectin-binding glycan in the fluid sample to a control level indicative to a level of the lectin-binding glycan in a body fluid of a subject not suffering from TBI, wherein said lectin-binding glucan is indicative to TBI when its level in the fluid sample is at least two times higher than the control level.