A manufacturing method of a waterproof glove, the manufacturing method comprising: a membrane glove manufacturing step of manufacturing a glove using a membrane sheet applied with a hot-melt adhesive; a lining glove inserting step of inserting a lining glove into the membrane glove manufactured to the membrane glove manufacturing step; a membrane glove inserting step of inserting the membrane glove with the lining glove inserted through the lining glove inserting step into an outshell glove; and a thermal bonding step of bonding the lining glove, the membrane glove, and the outshell glove by heating the outshell glove with the membrane glove inserted through the membrane glove inserting step, wherein the hot-melt adhesive is applied in a dot type characterized in that the membrane sheet is a polyurethane membrane sheet and in that the hot-melt adhesive is composed of olefin-based resin of 100 part by weight including a propylene repeat unit having a melting point of 70 to 120°C, hydrogenated styrene-based block copolymer of 50 to 400 part by weight, and hydrocarbon-based adhesive resin of 400 to 800 part by weight, the membrane glove manufacturing step is performed by applying a hot-melt adhesive to both sides of a polyurethane membrane sheet, stacking two polyurethane membrane sheets applied with a hot-melt adhesive and then cutting the sheets into a hand shape, and thermally bonding the edge of the stack of cut membrane sheets except for the portion in which the lining glove is inserted, the thermal bonding step is performed by putting the outshell glove with the membrane glove inserted through the membrane glove inserting step on a hand-shaped mold, and then heating the mold at temperature of 110 to 130°C. a membrane glove manufacturing step of manufacturing a glove using a membrane sheet applied with a hot-melt adhesive; a lining glove inserting step of inserting a lining glove into the membrane glove manufactured to the membrane glove manufacturing step; a membrane glove inserting step of inserting the membrane glove with the lining glove inserted through the lining glove inserting step into an outshell glove; and a thermal bonding step of bonding the lining glove, the membrane glove, and the outshell glove by heating the outshell glove with the membrane glove inserted through the membrane glove inserting step, wherein the hot-melt adhesive is applied in a dot type characterized in that the membrane sheet is a polyurethane membrane sheet and in that the hot-melt adhesive is composed of olefin-based resin of 100 part by weight including a propylene repeat unit having a melting point of 70 to 120°C, hydrogenated styrene-based block copolymer of 50 to 400 part by weight, and hydrocarbon-based adhesive resin of 400 to 800 part by weight, the membrane glove manufacturing step is performed by applying a hot-melt adhesive to both sides of a polyurethane membrane sheet, stacking two polyurethane membrane sheets applied with a hot-melt adhesive and then cutting the sheets into a hand shape, and thermally bonding the edge of the stack of cut membrane sheets except for the portion in which the lining glove is inserted, the thermal bonding step is performed by putting the outshell glove with the membrane glove inserted through the membrane glove inserting step on a hand-shaped mold, and then heating the mold at temperature of 110 to 130°C.