A high pressure freezing cartridge (300, 400, 500) for use in vitrification of a biological sample, the cartridge (300, 400, 500) being adapted to fixedly hold a sample chamber (216, 218, 220) at a sample chamber position (320, 420, 520) inside the cartridge (300, 400, 500), a refrigerant channel arrangement comprising at least one refrigerant channel (208, 308, 408, 462, 464, 466, 468, 508, 562, 564, 566, 569) being provided in the cartridge (300, 400, 500) and extending from a surface of the cartridge (300, 400, 500) to the sample chamber position, and the cartridge (300, 400, 500) comprising a baffle structure (350, 450, 550, 571) at a baffle position being adapted to interact with a refrigerant stream in the refrigerant channel arrangement before the refrigerant of the refrigerant stream reaches the sample chamber position (320, 420, 520) in the cartridge (300, 400, 500), wherein the cartridge (300, 400, 500) is formed using cartridge elements (302, 304, 306, 402, 404, 406, 502, 504, 506) being brought into an operational configuration, the cartridge elements (302, 304, 306, 402, 404, 406, 502, 504, 506) including two holding elements (302, 304, 402, 404, 502, 504) and a middle plate (306, 406, 506), the middle plate (306, 406, 506) being adapted to be sandwiched between the holding elements (302, 304, 402, 404, 502, 504) in the operational configuration of the cartridge elements (302, 304, 306, 402, 404, 406, 502, 504, 506); wherein the middle plate (306, 406, 506) is a flat element comprising an opening (316, 416, 516) adapted to form the sample chamber (216, 218, 220) by fitting enclosing elements into the opening (316, 416, 516); and characterized in that the baffle structure (350, 450, 550) is configured as a flat disc with an engaging element (351, 451, 551), the baffle structure (350, 450, 550) being positioned or fixedly held by at least one of the cartridge elements (302, 304, 306, 402, 404, 406, 502, 504, 506) at the baffle position by engaging the engaging element (351, 451, 551) to the respective cartridge element (302, 304, 306, 402, 404, 406, 502, 504, 506). wherein the cartridge (300, 400, 500) is formed using cartridge elements (302, 304, 306, 402, 404, 406, 502, 504, 506) being brought into an operational configuration, the cartridge elements (302, 304, 306, 402, 404, 406, 502, 504, 506) including two holding elements (302, 304, 402, 404, 502, 504) and a middle plate (306, 406, 506), the middle plate (306, 406, 506) being adapted to be sandwiched between the holding elements (302, 304, 402, 404, 502, 504) in the operational configuration of the cartridge elements (302, 304, 306, 402, 404, 406, 502, 504, 506); wherein the middle plate (306, 406, 506) is a flat element comprising an opening (316, 416, 516) adapted to form the sample chamber (216, 218, 220) by fitting enclosing elements into the opening (316, 416, 516); and characterized in that the baffle structure (350, 450, 550) is configured as a flat disc with an engaging element (351, 451, 551), the baffle structure (350, 450, 550) being positioned or fixedly held by at least one of the cartridge elements (302, 304, 306, 402, 404, 406, 502, 504, 506) at the baffle position by engaging the engaging element (351, 451, 551) to the respective cartridge element (302, 304, 306, 402, 404, 406, 502, 504, 506).