A method for determining the presence or absence of Candida species (sp.) in a sample, the method comprising: contacting a sample, said sample suspected of containing Candida sp., with a first amplification oligomer combination comprising, first and second Candida-specific amplification oligomers for amplifying a first Candida sp. target nucleic acid region or a second Candida sp. target nucleic acid region, wherein said first target region corresponds to a region of SEQ ID NO: 129 from about nucleotide position 133 or 161 to about nucleotide position 259 and said second region corresponds to a region of SEQ ID NO: 130 from about nucleotide position 202 to about nucleotide position 308, and wherein the first and second Candida-specific amplification oligomers respectively comprise first and second Candida-specific target-hybridizing sequences, wherein first Candida-specific target-hybridizing sequence consisting of the nucleotide sequence of residues 28-46 of SEQ ID NO:9; and the second Candida-specific target-hybridizing sequence consisting of SEQ ID NO: 26; and performing an in vitro nucleic acid amplification reaction, wherein any Candida sp. target nucleic acid, if present in the sample, is used as a template for generating one or more amplification products corresponding to at least one of the first, second, and third target regions; and detecting the presence or absence of the one or more amplification products, thereby determining the presence or absence of Candida sp. in the sample. contacting a sample, said sample suspected of containing Candida sp., with a first amplification oligomer combination comprising, first and second Candida-specific amplification oligomers for amplifying a first Candida sp. target nucleic acid region or a second Candida sp. target nucleic acid region, wherein said first target region corresponds to a region of SEQ ID NO: 129 from about nucleotide position 133 or 161 to about nucleotide position 259 and said second region corresponds to a region of SEQ ID NO: 130 from about nucleotide position 202 to about nucleotide position 308, and wherein the first and second Candida-specific amplification oligomers respectively comprise first and second Candida-specific target-hybridizing sequences, wherein first Candida-specific target-hybridizing sequence consisting of the nucleotide sequence of residues 28-46 of SEQ ID NO:9; and the second Candida-specific target-hybridizing sequence consisting of SEQ ID NO: 26; and first and second Candida-specific amplification oligomers for amplifying a first Candida sp. target nucleic acid region or a second Candida sp. target nucleic acid region, wherein said first target region corresponds to a region of SEQ ID NO: 129 from about nucleotide position 133 or 161 to about nucleotide position 259 and said second region corresponds to a region of SEQ ID NO: 130 from about nucleotide position 202 to about nucleotide position 308, and wherein the first and second Candida-specific amplification oligomers respectively comprise first and second Candida-specific target-hybridizing sequences, wherein first Candida-specific target-hybridizing sequence consisting of the nucleotide sequence of residues 28-46 of SEQ ID NO:9; and the second Candida-specific target-hybridizing sequence consisting of SEQ ID NO: 26; and performing an in vitro nucleic acid amplification reaction, wherein any Candida sp. target nucleic acid, if present in the sample, is used as a template for generating one or more amplification products corresponding to at least one of the first, second, and third target regions; and detecting the presence or absence of the one or more amplification products, thereby determining the presence or absence of Candida sp. in the sample.