A method of determining the concentration of at least one compound in a chromatography method comprising the steps of (ia) selecting the at least one compound; (ib) selecting a stationary phase; (ic) selecting a mobile phase; (id) selecting a chromatography device having a chromatography bed comprising the stationary phase and the mobile phase; (iia) obtaining an adsorption isotherm of the at least one compound on the stationary phase; (iib) obtaining the maximum diffusion time t diff of the at least one compound in the mobile phase; (iii) selecting a minimum residence time t res of the at least one compound in the chromatography bed such that Equation I is satisfied t res > t diff (iv) calculating a concentration c ( z, t) of the at least one compound in the mobile phase at a predetermined location z of the chromatography device and at a predetermined time t based on the adsorption isotherm, the predetermined location z of the chromatography device being along the predetermined direction in which the mobile phase moves during the chromatography, wherein step (iv) is carried out without taking the diffusion of the at least one compound in the mobile phase into account, wherein the maximum diffusion time t diff describes the maximum time needed for a target molecule in the center of a pore or of a transport channel of the stationary phase to reach a binding site by diffusion and is determined as t diff =(x(t)) 2 /(2D eff), wherein D eff is the effective diffusion coefficient, and x(t) is the maximum diffusion path length for a target molecule to reach a binding site; and wherein the minimum residence time t res of the at least one compound is calculated using the volumetric flow rate F, the volume of the chromatography bed defined by its geometric dimensions V b and the total porosity ε T t res = V b ε T F. (ia) selecting the at least one compound; (ib) selecting a stationary phase; (ic) selecting a mobile phase; (id) selecting a chromatography device having a chromatography bed comprising the stationary phase and the mobile phase; (iia) obtaining an adsorption isotherm of the at least one compound on the stationary phase; (iib) obtaining the maximum diffusion time t diff of the at least one compound in the mobile phase; (iii) selecting a minimum residence time t res of the at least one compound in the chromatography bed such that Equation I is satisfied t res > t diff (iv) calculating a concentration c ( z, t) of the at least one compound in the mobile phase at a predetermined location z of the chromatography device and at a predetermined time t based on the adsorption isotherm, the predetermined location z of the chromatography device being along the predetermined direction in which the mobile phase moves during the chromatography, wherein step (iv) is carried out without taking the diffusion of the at least one compound in the mobile phase into account, wherein the maximum diffusion time t diff describes the maximum time needed for a target molecule in the center of a pore or of a transport channel of the stationary phase to reach a binding site by diffusion and is determined as t diff =(x(t)) 2 /(2D eff), wherein D eff is the effective diffusion coefficient, and x(t) is the maximum diffusion path length for a target molecule to reach a binding site; and wherein the minimum residence time t res of the at least one compound is calculated using the volumetric flow rate F, the volume of the chromatography bed defined by its geometric dimensions V b and the total porosity ε T t res = V b ε T F.