A reaction cassette for a glycated hemoglobin meter, the reaction cassette comprising: a first zone for receiving a reagent and a blood sample, wherein the reagent includes a hemolysate and glycated hemoglobin binding material-beads, and selectively reacts with glycated hemoglobin, a second zone for receiving a washing solution, wherein the first zone is a reaction zone, in which the blood sample reacts with the reagent and through which the washing solution passes to wash the blood sample mixture obtained by reacting the blood sample with the reagent, a measurement zone for measuring an amount of total hemoglobin in the blood sample mixture of the reagent and the blood sample and for measuring an amount of glycated hemoglobin in the blood sample mixture from which non-glycated hemoglobin is removed, and a reagent absorption unit disposed below the measurement zone for absorbing the non-glycated hemoglobin and remaining materials of the blood sample mixture except for glycated hemoglobin binding materials-beads combined with glycated hemoglobin, which exist in the measurement zone, after the blood sample mixture is washed with the washing solution; wherein a first and second partition wall (132, 134) are arranged around the second zone such that the first partitioning wall separates the second zone from the measurement zone and prevents the washing solution in the second zone from moving to the first zone within a specific rotation angle range, and such that the second partitioning wall prevents the washing solution in the second zone from moving to any other zone within a specific rotation angle; said reaction cassette further comprises a delivery guide unit for guiding the blood sample mixture so as to move from the first zone to the measurement zone according to a rotation of the reaction cassette while preventing the washing solution from moving from the second zone to the first zone; and wherein the reaction cassette is adapted that upon rotation of the reaction cassette, the blood sample mixture is moved from the first zone to the measurement zone, and the washing solution is moved from the second zone through the first zone to the measurement zone so that the washing solution washes the non-glycated hemoglobin from the blood sample mixture in the measurement zone.. a first zone for receiving a reagent and a blood sample, wherein the reagent includes a hemolysate and glycated hemoglobin binding material-beads, and selectively reacts with glycated hemoglobin, a second zone for receiving a washing solution, wherein the first zone is a reaction zone, in which the blood sample reacts with the reagent and through which the washing solution passes to wash the blood sample mixture obtained by reacting the blood sample with the reagent, a measurement zone for measuring an amount of total hemoglobin in the blood sample mixture of the reagent and the blood sample and for measuring an amount of glycated hemoglobin in the blood sample mixture from which non-glycated hemoglobin is removed, and a reagent absorption unit disposed below the measurement zone for absorbing the non-glycated hemoglobin and remaining materials of the blood sample mixture except for glycated hemoglobin binding materials-beads combined with glycated hemoglobin, which exist in the measurement zone, after the blood sample mixture is washed with the washing solution; wherein a first and second partition wall (132, 134) are arranged around the second zone such that the first partitioning wall separates the second zone from the measurement zone and prevents the washing solution in the second zone from moving to the first zone within a specific rotation angle range, and such that the second partitioning wall prevents the washing solution in the second zone from moving to any other zone within a specific rotation angle; said reaction cassette further comprises a delivery guide unit for guiding the blood sample mixture so as to move from the first zone to the measurement zone according to a rotation of the reaction cassette while preventing the washing solution from moving from the second zone to the first zone; and wherein the reaction cassette is adapted that upon rotation of the reaction cassette, the blood sample mixture is moved from the first zone to the measurement zone, and the washing solution is moved from the second zone through the first zone to the measurement zone so that the washing solution washes the non-glycated hemoglobin from the blood sample mixture in the measurement zone..