A guidance program for guiding a camera, which is held by a holder and which captures a field of view of a microscope through an eyepiece lens of the microscope, to an appropriate holding position with respect to an optical axis of the eyepiece lens, the guidance program causing a computer to function as: an image acquisition unit that acquires, from the camera, an image of the field of view captured by the camera held by the holder; a specification unit that specifies a visual field area in the image, the visual field area corresponding to the field of view; a center of gravity coordinate acquisition unit that obtains a luminance center of gravity coordinate of the visual field area; a guidance information creation unit that creates guidance information to guide the camera to the appropriate holding position based on a distance between the luminance center of gravity coordinate and a reference coordinate corresponding to a center of gravity of the visual field area obtained from an image captured by the camera held at the appropriate holding position; and an output unit that outputs the guidance information, wherein: the appropriate holding position is a holding position of the camera at which an optical axis of the eyepiece lens and an optical axis of the camera are matched, each of the luminance center of gravity coordinate and the reference coordinate is represented by a first coordinate and a second coordinate representing a two-dimensional position on an image, and the guidance information creation unit creates the guidance information in a case in which at least one of a first value representing an absolute value of a difference between the first coordinate of the luminance center of gravity coordinate and the first coordinate of the reference coordinate, or a second value representing an absolute value of a difference between the second coordinate of the luminance center of gravity coordinate and the second coordinate of the reference coordinate, is outside a predetermined range, and the guidance information creation unit does not create the guidance information in a case in which both of the first value and the second value are within the predetermined range. an image acquisition unit that acquires, from the camera, an image of the field of view captured by the camera held by the holder; a specification unit that specifies a visual field area in the image, the visual field area corresponding to the field of view; a center of gravity coordinate acquisition unit that obtains a luminance center of gravity coordinate of the visual field area; a guidance information creation unit that creates guidance information to guide the camera to the appropriate holding position based on a distance between the luminance center of gravity coordinate and a reference coordinate corresponding to a center of gravity of the visual field area obtained from an image captured by the camera held at the appropriate holding position; and an output unit that outputs the guidance information, wherein: the appropriate holding position is a holding position of the camera at which an optical axis of the eyepiece lens and an optical axis of the camera are matched, each of the luminance center of gravity coordinate and the reference coordinate is represented by a first coordinate and a second coordinate representing a two-dimensional position on an image, and the guidance information creation unit creates the guidance information in a case in which at least one of a first value representing an absolute value of a difference between the first coordinate of the luminance center of gravity coordinate and the first coordinate of the reference coordinate, or a second value representing an absolute value of a difference between the second coordinate of the luminance center of gravity coordinate and the second coordinate of the reference coordinate, is outside a predetermined range, and the guidance information creation unit does not create the guidance information in a case in which both of the first value and the second value are within the predetermined range. the appropriate holding position is a holding position of the camera at which an optical axis of the eyepiece lens and an optical axis of the camera are matched, each of the luminance center of gravity coordinate and the reference coordinate is represented by a first coordinate and a second coordinate representing a two-dimensional position on an image, and the guidance information creation unit creates the guidance information in a case in which at least one of a first value representing an absolute value of a difference between the first coordinate of the luminance center of gravity coordinate and the first coordinate of the reference coordinate, or a second value representing an absolute value of a difference between the second coordinate of the luminance center of gravity coordinate and the second coordinate of the reference coordinate, is outside a predetermined range, and the guidance information creation unit does not create the guidance information in a case in which both of the first value and the second value are within the predetermined range.