Method of selecting a yeast strain that is resistant to a toxic medium, comprising: - a step of pre-selection of at least one yeast strain for which the maximum amount of alcohol produced, in a non-toxic selection medium under non-limiting sugar conditions, is greater than or equal to 92% of the maximum amount of alcohol produced, in the non-toxic selection medium, by the reference strain filed on 4 September 2008 at the CNCM [French National Collection of Microorganism Cultures] under number I-4071, - a step of fermentation, in a toxic selection medium and in a non-toxic selection medium, of the at least one pre-selected yeast strain, the initial composition of the toxic selection medium being the initial composition of the non-toxic selection medium to which has been added at least one non-ionized organic acid chosen from acetic acid, lactic acid, formic acid, levulinic acid or a mixture thereof, and the sugar being the limiting factor of the fermentation in the toxic selection medium and in the non-toxic selection medium, wherein the concentration of the non-ionized organic acid in the toxic selection medium is a concentration which leads to a decrease in the amount of alcohol produced at time t max ref by the reference strain number 1-4071 of 25% to 75%, preferably a decrease of 40% to 75%, relative to the maximum amount of alcohol produced by said reference strain of number 1-4071 at time t max ref in the non-toxic selection medium, wherein the time t max ref is the time at which the maximum amount of alcohol produced by said reference strain number 1-4071 is reached in the non-toxic selection medium, - a step of selection of at least one yeast strain for which the amount of alcohol produced at time t max in the toxic selection medium is greater than or equal to 65% of the amount of alcohol produced at time t max by said strain in the non-toxic selection medium, wherein the time t max is the time at which the maximum amount of alcohol produced by said yeast strain is reached in the non-toxic selection medium, and - an additional step of selection of at least one yeast strain for which the amount of alcohol produced at time t max in said toxic selection medium is greater than the amount of alcohol produced at time t max by the reference yeast strain number 1-4071 in said same toxic selection medium. - a step of pre-selection of at least one yeast strain for which the maximum amount of alcohol produced, in a non-toxic selection medium under non-limiting sugar conditions, is greater than or equal to 92% of the maximum amount of alcohol produced, in the non-toxic selection medium, by the reference strain filed on 4 September 2008 at the CNCM [French National Collection of Microorganism Cultures] under number I-4071, - a step of fermentation, in a toxic selection medium and in a non-toxic selection medium, of the at least one pre-selected yeast strain, the initial composition of the toxic selection medium being the initial composition of the non-toxic selection medium to which has been added at least one non-ionized organic acid chosen from acetic acid, lactic acid, formic acid, levulinic acid or a mixture thereof, and the sugar being the limiting factor of the fermentation in the toxic selection medium and in the non-toxic selection medium, wherein the concentration of the non-ionized organic acid in the toxic selection medium is a concentration which leads to a decrease in the amount of alcohol produced at time t max ref by the reference strain number 1-4071 of 25% to 75%, preferably a decrease of 40% to 75%, relative to the maximum amount of alcohol produced by said reference strain of number 1-4071 at time t max ref in the non-toxic selection medium, wherein the time t max ref is the time at which the maximum amount of alcohol produced by said reference strain number 1-4071 is reached in the non-toxic selection medium, the initial composition of the toxic selection medium being the initial composition of the non-toxic selection medium to which has been added at least one non-ionized organic acid chosen from acetic acid, lactic acid, formic acid, levulinic acid or a mixture thereof, and the sugar being the limiting factor of the fermentation in the toxic selection medium and in the non-toxic selection medium, wherein the concentration of the non-ionized organic acid in the toxic selection medium is a concentration which leads to a decrease in the amount of alcohol produced at time t max ref by the reference strain number 1-4071 of 25% to 75%, preferably a decrease of 40% to 75%, relative to the maximum amount of alcohol produced by said reference strain of number 1-4071 at time t max ref in the non-toxic selection medium, wherein the time t max ref is the time at which the maximum amount of alcohol produced by said reference strain number 1-4071 is reached in the non-toxic selection medium, - a step of selection of at least one yeast strain for which the amount of alcohol produced at time t max in the toxic selection medium is greater than or equal to 65% of the amount of alcohol produced at time t max by said strain in the non-toxic selection medium, wherein the time t max is the time at which the maximum amount of alcohol produced by said yeast strain is reached in the non-toxic selection medium, and - an additional step of selection of at least one yeast strain for which the amount of alcohol produced at time t max in said toxic selection medium is greater than the amount of alcohol produced at time t max by the reference yeast strain number 1-4071 in said same toxic selection medium.