A tape cassette having a tape of long length and for placement in a tape printing apparatus in a replaceable manner, the tape printing apparatus comprising a tape conveying device for conveying the tape and a printing device for carrying out printing on the tape, the tape conveying device including a tape sub roller arranged so as to rotate therearound and to press against the tape, the tape cassette comprising: a tape ejecting port for ejecting the tape outside of the tape cassette, the tape having a separator adhered to one of the surfaces thereof; a tape conveying roller arranged, with respect to a tape conveying direction, at an upstream side of the tape ejecting port for pressing the tape sub roller on the tape with the separator adhered to one of the surfaces thereof, the tape conveying roller being configured to pull out and convey the tape while getting in contact with the separator; a separator guiding portion for guiding the separator, peeled off from the tape, inwardly at the tape ejecting port along a peripheral surface of the tape conveying roller; and a separator-take-up spool arranged to fix thereto a front end of the separator that has been guided inwardly along the peripheral surface of the tape conveying roller from the separator guiding portion and to take up the separator; wherein: the separator guiding portion includes a separator entrance formed so as to penetrate one of side wall portions that gets in contact with the separator adhered to the tape at the tape ejecting port, the separator entrance allowing entering of the separator peeled off from the tape, and the separator peeled off from the tape at an outer edge portion of the tape ejecting port is allowed to enter from the separator entrance and guided toward the peripheral surface of the tape conveying roller. a tape ejecting port for ejecting the tape outside of the tape cassette, the tape having a separator adhered to one of the surfaces thereof; a tape conveying roller arranged, with respect to a tape conveying direction, at an upstream side of the tape ejecting port for pressing the tape sub roller on the tape with the separator adhered to one of the surfaces thereof, the tape conveying roller being configured to pull out and convey the tape while getting in contact with the separator; a separator guiding portion for guiding the separator, peeled off from the tape, inwardly at the tape ejecting port along a peripheral surface of the tape conveying roller; and a separator-take-up spool arranged to fix thereto a front end of the separator that has been guided inwardly along the peripheral surface of the tape conveying roller from the separator guiding portion and to take up the separator; wherein: the separator guiding portion includes a separator entrance formed so as to penetrate one of side wall portions that gets in contact with the separator adhered to the tape at the tape ejecting port, the separator entrance allowing entering of the separator peeled off from the tape, and the separator peeled off from the tape at an outer edge portion of the tape ejecting port is allowed to enter from the separator entrance and guided toward the peripheral surface of the tape conveying roller. the separator guiding portion includes a separator entrance formed so as to penetrate one of side wall portions that gets in contact with the separator adhered to the tape at the tape ejecting port, the separator entrance allowing entering of the separator peeled off from the tape, and the separator peeled off from the tape at an outer edge portion of the tape ejecting port is allowed to enter from the separator entrance and guided toward the peripheral surface of the tape conveying roller.