A transgenic Yarrowia lipolytica cell comprising a modification that results in: a) a heterotrimeric SNF1 protein kinase having reduced activity when compared to the activity of a heterotrimeric SNF1 protein kinase of a non-transgenic Yarrowia lipolytica cell not having said modification; or b) an alteration to a downstream protein regulated by the heterotrimeric SNF1 protein kinase; wherein the modification is selected from the group consisting of: i. down-regulation of an upstream regulatory protein associated with the heterotrimeric SNF1 protein kinase, said upstream regulatory protein being the kinase Sak1; ii. up-regulation of an upstream regulatory protein associated with the heterotrimeric SNF1 protein kinase, said upstream regulatory protein being a hexokinase consisting of hexokinase isoenzyme 2; iii. up-regulation of an upstream regulatory protein associated with the heterotrimeric SNF1 protein kinase, said upstream regulatory protein being glucokinase Glk1; iv. up-regulation of an upstream regulatory protein associated with the heterotrimeric SNF1 protein kinase, said upstream regulatory protein being Reg1; v. down-regulation of a polynucleotide encoding the SNF1 α-subunit of the heterotrimeric SNF1 protein kinase; vi. up-regulation of the regulatory domain of a polynucleotide encoding the SNF1 α-subunit of the heterotrimeric SNF1 protein kinase; vii. up-regulation of a catalytically inactive Snf1 α-subunit; viii. down-regulation of a polynucleotide encoding the SNF1 β-subunit of the heterotrimeric SNF1 protein kinase, said β-subunit consisting of Gal83; ix. down-regulation of a polynucleotide encoding the SNF1 γ-subunit of the heterotrimeric SNF1 protein kinase; x. up-regulation of a downstream protein regulated by the heterotrimeric SNF1 protein kinase, said downstream protein being the zinc-finger protein Rme1; xi. up-regulation of a downstream protein regulated by the heterotrimeric SNF1 protein kinase, said downstream protein being microsomal cytochrome b 5 reductase Cbr1; and xii. up-regulation of a downstream protein regulated by the heterotrimeric SNF1 protein kinase, said downstream protein being glucose transporter Snf3; and wherein said modification results in an increase in total lipid content when compared to the total lipid content of a non-transgenic Yarrowia lipolytica cell not having said modification. a) a heterotrimeric SNF1 protein kinase having reduced activity when compared to the activity of a heterotrimeric SNF1 protein kinase of a non-transgenic Yarrowia lipolytica cell not having said modification; or b) an alteration to a downstream protein regulated by the heterotrimeric SNF1 protein kinase; i. down-regulation of an upstream regulatory protein associated with the heterotrimeric SNF1 protein kinase, said upstream regulatory protein being the kinase Sak1; ii. up-regulation of an upstream regulatory protein associated with the heterotrimeric SNF1 protein kinase, said upstream regulatory protein being a hexokinase consisting of hexokinase isoenzyme 2; iii. up-regulation of an upstream regulatory protein associated with the heterotrimeric SNF1 protein kinase, said upstream regulatory protein being glucokinase Glk1; iv. up-regulation of an upstream regulatory protein associated with the heterotrimeric SNF1 protein kinase, said upstream regulatory protein being Reg1; v. down-regulation of a polynucleotide encoding the SNF1 α-subunit of the heterotrimeric SNF1 protein kinase; vi. up-regulation of the regulatory domain of a polynucleotide encoding the SNF1 α-subunit of the heterotrimeric SNF1 protein kinase; vii. up-regulation of a catalytically inactive Snf1 α-subunit; viii. down-regulation of a polynucleotide encoding the SNF1 β-subunit of the heterotrimeric SNF1 protein kinase, said β-subunit consisting of Gal83; ix. down-regulation of a polynucleotide encoding the SNF1 γ-subunit of the heterotrimeric SNF1 protein kinase; x. up-regulation of a downstream protein regulated by the heterotrimeric SNF1 protein kinase, said downstream protein being the zinc-finger protein Rme1; xi. up-regulation of a downstream protein regulated by the heterotrimeric SNF1 protein kinase, said downstream protein being microsomal cytochrome b 5 reductase Cbr1; and xii. up-regulation of a downstream protein regulated by the heterotrimeric SNF1 protein kinase, said downstream protein being glucose transporter Snf3; and