An apparatus for non-invasive glucose monitoring, comprising: at least one first light source, emitting at least one ray of first light; a first beam splitter with a focusing function, leading the first light emitted from the first light source into an eyeball and focusing on the eyeball through the first beam splitter; a set of photo detectors, measuring an optical rotatory distribution information and an absorption energy information of the first light reflected from the eyeball and then transmitted through the first beam splitter to the set of photo detectors, the first light emitted from the first light source being transmitted to the set of photo detectors through the first beam splitter and the eyeball so as to form an optical path; a processing unit, receiving and processing the optical rotatory distribution information and the absorption energy information to obtain a glucose information; and an eye positioning device, comprising: a second beam splitter disposed on the optical path between the first beam splitter and the eyeball; and a camera, receiving an image information transmitted from the second beam splitter; wherein the processing unit obtains an optical rotatory distribution difference and an absorption energy difference between the first light emitted from the first light source and the first light transmitted to the set of photo detectors, so as to obtain a biological molecule information of a biological molecule, the biological molecule at least comprises a glucose, the processing unit obtains the glucose information through the biological molecule information, and a blood glucose information is read since the glucose information and the blood glucose i nformati on have a corresponding relationship. at least one first light source, emitting at least one ray of first light; a first beam splitter with a focusing function, leading the first light emitted from the first light source into an eyeball and focusing on the eyeball through the first beam splitter; a set of photo detectors, measuring an optical rotatory distribution information and an absorption energy information of the first light reflected from the eyeball and then transmitted through the first beam splitter to the set of photo detectors, the first light emitted from the first light source being transmitted to the set of photo detectors through the first beam splitter and the eyeball so as to form an optical path; a processing unit, receiving and processing the optical rotatory distribution information and the absorption energy information to obtain a glucose information; and an eye positioning device, comprising: a second beam splitter disposed on the optical path between the first beam splitter and the eyeball; and a camera, receiving an image information transmitted from the second beam splitter; a second beam splitter disposed on the optical path between the first beam splitter and the eyeball; and a camera, receiving an image information transmitted from the second beam splitter; wherein the processing unit obtains an optical rotatory distribution difference and an absorption energy difference between the first light emitted from the first light source and the first light transmitted to the set of photo detectors, so as to obtain a biological molecule information of a biological molecule, the biological molecule at least comprises a glucose, the processing unit obtains the glucose information through the biological molecule information, and a blood glucose information is read since the glucose information and the blood glucose i nformati on have a corresponding relationship.