A biochip, which upon insertion into an electrophoresis instrument having pneumatic, thermal, high voltage, and optical subsystems, and a process controller, generates a nucleic acid sequencing or sizing profile from at least one unprocessed sample, said biochip comprising: a macrofluidic processing subassembly, a fluidic subassembly, a pneumatic subassembly, and a separation and detection subassembly, wherein the macrofluidic processing subassembly is in connection with the fluidic subassembly and the pneumatic subassembly and comprises at least one chamber adapted to receive a sample; the fluidic subassembly comprises a fluidic plate with at least one fluid transport channel and an amplification chamber adapted to connect to the thermal subsystem of said instrument; the separation and detection subassembly is adapted for connection to the high voltage, the optical subsystems and the process controller of said instrument, said separation and detection subassembly comprising a separation channel, and further comprising a detection region positioned to send signals from each of said channels to said optical subsystem on said instrument; the pneumatic subassembly is adapted to connect to the pneumatic subsystem of said instrument, and is connected to the subassemblies of said biochip, the pneumatic subsystem comprising, a pneumatic plate and one or a plurality of drive lines to pneumatically drive fluids on instructions from the process controller of said instrument; wherein the biochip is plastic, stationary, and unitary, and a footprint of the fluidic and/or pneumatic plates is (i) about 86 mm by 128 mm or greater or (ii) is less than 10,920.0 mm 2 and does not conform to SBS standards, and wherein the biochip generates a nucleic acid sequencing or sizing profile from the at least one unprocessed sample. a macrofluidic processing subassembly, a fluidic subassembly, a pneumatic subassembly, and a separation and detection subassembly, wherein the macrofluidic processing subassembly is in connection with the fluidic subassembly and the pneumatic subassembly and comprises at least one chamber adapted to receive a sample; the fluidic subassembly comprises a fluidic plate with at least one fluid transport channel and an amplification chamber adapted to connect to the thermal subsystem of said instrument; the separation and detection subassembly is adapted for connection to the high voltage, the optical subsystems and the process controller of said instrument, said separation and detection subassembly comprising a separation channel, and further comprising a detection region positioned to send signals from each of said channels to said optical subsystem on said instrument; the pneumatic subassembly is adapted to connect to the pneumatic subsystem of said instrument, and is connected to the subassemblies of said biochip, the pneumatic subsystem comprising, a pneumatic plate and one or a plurality of drive lines to pneumatically drive fluids on instructions from the process controller of said instrument; wherein the biochip is plastic, stationary, and unitary, and a footprint of the fluidic and/or pneumatic plates is (i) about 86 mm by 128 mm or greater or (ii) is less than 10,920.0 mm 2 and does not conform to SBS standards, and wherein the biochip generates a nucleic acid sequencing or sizing profile from the at least one unprocessed sample.