A method of producing an isolated cultured milk product from mammary cells, the method comprising: (a) culturing a live cell construct in a bioreactor under conditions which produce the cultured milk product, said live cell construct comprising: (i) an at least 70% confluent monolayer of polarized mammary cells, wherein the mammary cells are selected from the group consisting of: live primary mammary epithelial cells, live mammary myoepithelial cells, live mammary progenitor cells, live immortalized mammary epithelial cells, live immortalized mammary myoepithelial cells, and live immortalized mammary progenitor cells, and wherein the polarized mammary cells comprise an apical surface from which the cultured milk product is secreted and a basal surface; (ii) a three-dimensional scaffold having an exterior surface, an interior surface defining an interior cavity/basal chamber, and a plurality of pores extending from the interior surface to the exterior surface; (iii) a matrix material disposed on the exterior surface of the three-dimensional scaffold, wherein the at least 70% confluent monolayer of polarized cells is disposed upon the matrix material; and (iv) a culture media disposed within the interior cavity/basal chamber and in fluidic contact with the internal surface of the three dimensional scaffold and the basal surface of the polarized mammary cells, wherein the culture media substantially does not contact the cultured milk product; and said bioreactor comprising an apical compartment that is in fluidic contact with the apical surface of the mammary cells and is substantially isolated from the internal cavity of the live cell construct and the cell culture media disposed within the interior cavity; and (b) isolating the cultured milk product secreted into the apical compartment from the apical surface of the mammary cells. (a) culturing a live cell construct in a bioreactor under conditions which produce the cultured milk product, said live cell construct comprising: (i) an at least 70% confluent monolayer of polarized mammary cells, wherein the mammary cells are selected from the group consisting of: live primary mammary epithelial cells, live mammary myoepithelial cells, live mammary progenitor cells, live immortalized mammary epithelial cells, live immortalized mammary myoepithelial cells, and live immortalized mammary progenitor cells, and wherein the polarized mammary cells comprise an apical surface from which the cultured milk product is secreted and a basal surface; (ii) a three-dimensional scaffold having an exterior surface, an interior surface defining an interior cavity/basal chamber, and a plurality of pores extending from the interior surface to the exterior surface; (iii) a matrix material disposed on the exterior surface of the three-dimensional scaffold, wherein the at least 70% confluent monolayer of polarized cells is disposed upon the matrix material; and (iv) a culture media disposed within the interior cavity/basal chamber and in fluidic contact with the internal surface of the three dimensional scaffold and the basal surface of the polarized mammary cells, wherein the culture media substantially does not contact the cultured milk product; and said bioreactor comprising an apical compartment that is in fluidic contact with the apical surface of the mammary cells and is substantially isolated from the internal cavity of the live cell construct and the cell culture media disposed within the interior cavity; and (i) an at least 70% confluent monolayer of polarized mammary cells, wherein the mammary cells are selected from the group consisting of: live primary mammary epithelial cells, live mammary myoepithelial cells, live mammary progenitor cells, live immortalized mammary epithelial cells, live immortalized mammary myoepithelial cells, and live immortalized mammary progenitor cells, and wherein the polarized mammary cells comprise an apical surface from which the cultured milk product is secreted and a basal surface; (ii) a three-dimensional scaffold having an exterior surface, an interior surface defining an interior cavity/basal chamber, and a plurality of pores extending from the interior surface to the exterior surface; (iii) a matrix material disposed on the exterior surface of the three-dimensional scaffold, wherein the at least 70% confluent monolayer of polarized cells is disposed upon the matrix material; and (iv) a culture media disposed within the interior cavity/basal chamber and in fluidic contact with the internal surface of the three dimensional scaffold and the basal surface of the polarized mammary cells, wherein the culture media substantially does not contact the cultured milk product; and (b) isolating the cultured milk product secreted into the apical compartment from the apical surface of the mammary cells.