A genetically modified plant comprising an altered content of at least one cholesterol derived compound selected from a steroidal alkaloid or a glycosylated derivative thereof and an unsaturated or saturated steroidal saponin or a glycoside derivative thereof, wherein said steroidal alkaloid or glycosylated derivative thereof is selected from tomatidine, α-tomatine, α-tomatine isomer (1 and 2), α-tomatine isomer 1, α-tomatine isomer 2, hydroxytomatine, acetoxytomatine, dehydrotomatidine, dehydrotomatine, dehydrotomatine isomer 1, dehydrotomatine + 4-hexose, acetoxy-hydroxytomatine, acetoxy-hydroxy-dehydrotomatine, tomatidine + 4 hexose, esculeosides, esculeoside A, esculeoside A + hexose, esculeoside B, acetoxyesculeoside B, demissidine, demissine, dehydrosolasodine, hydroxy-dehydrotomatine, dehydroesculeosides, dehydroesculeoside A, dehydroesculeoside A + hexose, lycoperosides, soladulcidine, β-soladulcine, soladulcine A, solanidine, α-solanine, α-chaconine, solasodine, α-solasonine, and α-solamargine, and wherein said unsaturated or saturated steroidal saponin or a glycoside derivative thereof is selected from diosgenin, parillin, and sarasapogenin, said plant comprising at least one cell having an altered expression of 3-β-hydroxysteroid dehydrogenase/isomerase (GAME25), wherein said altered expression of GAME25 comprises a reduced or inhibited expression, or an enhanced expression, compared to GAME25 expression in a corresponding unmodified plant; wherein the at least one cell comprises: (a) a reduced content of said at least one steroidal alkaloid or a glycosylated derivative thereof or at least one unsaturated or saturated steroidal saponin or a glycosylated derivative thereof; (b) an increased content of said at least one steroidal alkaloid or a glycosylated derivative thereof or at least one unsaturated or saturated steroidal saponin or a glycosylated derivative thereof; (c) an appearance of said at least one steroidal alkaloid or a glycosylated derivative thereof or at least one unsaturated or saturated steroidal saponin or a glycosylated derivative thereof; or (d) any combination thereof; and wherein said plant is a Solanaceae crop plant. wherein said steroidal alkaloid or glycosylated derivative thereof is selected from tomatidine, α-tomatine, α-tomatine isomer (1 and 2), α-tomatine isomer 1, α-tomatine isomer 2, hydroxytomatine, acetoxytomatine, dehydrotomatidine, dehydrotomatine, dehydrotomatine isomer 1, dehydrotomatine + 4-hexose, acetoxy-hydroxytomatine, acetoxy-hydroxy-dehydrotomatine, tomatidine + 4 hexose, esculeosides, esculeoside A, esculeoside A + hexose, esculeoside B, acetoxyesculeoside B, demissidine, demissine, dehydrosolasodine, hydroxy-dehydrotomatine, dehydroesculeosides, dehydroesculeoside A, dehydroesculeoside A + hexose, lycoperosides, soladulcidine, β-soladulcine, soladulcine A, solanidine, α-solanine, α-chaconine, solasodine, α-solasonine, and α-solamargine, and wherein said unsaturated or saturated steroidal saponin or a glycoside derivative thereof is selected from diosgenin, parillin, and sarasapogenin, said plant comprising at least one cell having an altered expression of 3-β-hydroxysteroid dehydrogenase/isomerase (GAME25), wherein said altered expression of GAME25 comprises a reduced or inhibited expression, or an enhanced expression, compared to GAME25 expression in a corresponding unmodified plant; wherein the at least one cell comprises: (a) a reduced content of said at least one steroidal alkaloid or a glycosylated derivative thereof or at least one unsaturated or saturated steroidal saponin or a glycosylated derivative thereof; (b) an increased content of said at least one steroidal alkaloid or a glycosylated derivative thereof or at least one unsaturated or saturated steroidal saponin or a glycosylated derivative thereof; (c) an appearance of said at least one steroidal alkaloid or a glycosylated derivative thereof or at least one unsaturated or saturated steroidal saponin or a glycosylated derivative thereof; or (d) any combination thereof; and (a) a reduced content of said at least one steroidal alkaloid or a glycosylated derivative thereof or at least one unsaturated or saturated steroidal saponin or a glycosylated derivative thereof; (b) an increased content of said at least one steroidal alkaloid or a glycosylated derivative thereof or at least one unsaturated or saturated steroidal saponin or a glycosylated derivative thereof; (c) an appearance of said at least one steroidal alkaloid or a glycosylated derivative thereof or at least one unsaturated or saturated steroidal saponin or a glycosylated derivative thereof; or (d) any combination thereof; and wherein said plant is a Solanaceae crop plant.