A conscious animal ultrasonic neural regulation device, comprising a pulse signal generation module, a transducer module and a fixing module; wherein the pulse signal generation module is configured to generate a pulse signal; the ultrasonic transducer module is configured to convert the pulse signal into an ultrasound; wherein the fixing module comprises an upper fixing module and a lower fixing module; wherein the upper fixing module is configured to fix the ultrasonic transducer module, and the lower fixing module is configured to be fixed with an animal neural regulation target point; the upper fixing module and the lower fixing module are connected by a connecting component, wherein the pulse signal generation module comprises: an independent two-channel signal generator, configured to generate a neural regulation pulse waveform; a power amplifier, configured to increase an energy of the regulation pulse waveform; and an impedance matching circuit, configured to perform personalization design based on an actual measured impedance value of a piezoelectric array element, to realize impedance matching, wherein the ultrasonic transducer module comprises a leading wire, the piezoelectric array element and a backing; the piezoelectric array element is connected to the leading wire, and the leading wire is connected to the impedance matching circuit of the pulse signal generation module; the backing is made of an epoxy resin material, and provided with a water inlet and an air outlet, the water inlet is configured to inject deionized water serving as a coupling agent. wherein the pulse signal generation module is configured to generate a pulse signal; the ultrasonic transducer module is configured to convert the pulse signal into an ultrasound; wherein the fixing module comprises an upper fixing module and a lower fixing module; wherein the upper fixing module is configured to fix the ultrasonic transducer module, and the lower fixing module is configured to be fixed with an animal neural regulation target point; the upper fixing module and the lower fixing module are connected by a connecting component, wherein the pulse signal generation module comprises: an independent two-channel signal generator, configured to generate a neural regulation pulse waveform; a power amplifier, configured to increase an energy of the regulation pulse waveform; and an impedance matching circuit, configured to perform personalization design based on an actual measured impedance value of a piezoelectric array element, to realize impedance matching, wherein the ultrasonic transducer module comprises a leading wire, the piezoelectric array element and a backing; the piezoelectric array element is connected to the leading wire, and the leading wire is connected to the impedance matching circuit of the pulse signal generation module; the backing is made of an epoxy resin material, and provided with a water inlet and an air outlet, the water inlet is configured to inject deionized water serving as a coupling agent. an independent two-channel signal generator, configured to generate a neural regulation pulse waveform; a power amplifier, configured to increase an energy of the regulation pulse waveform; and an impedance matching circuit, configured to perform personalization design based on an actual measured impedance value of a piezoelectric array element, to realize impedance matching, wherein the ultrasonic transducer module comprises a leading wire, the piezoelectric array element and a backing; the piezoelectric array element is connected to the leading wire, and the leading wire is connected to the impedance matching circuit of the pulse signal generation module; the backing is made of an epoxy resin material, and provided with a water inlet and an air outlet, the water inlet is configured to inject deionized water serving as a coupling agent.