A method for preparing a diene-based rubber latex comprising: a step of adding 60 parts by weight to 75 parts by weight of a conjugated diene-based monomer, 1 part by weight to 3 parts by weight of a first emulsifier, 0.2 part by weight to 0.4 part by weight of a polymerization initiator, 0.2 part by weight to 3 parts by weight of an electrolyte, 0.1 part by weight to 0.5 part by weight of a molecular weight control agent and 65 parts by weight to 100 parts by weight of ion exchanged water into a reactor and then polymerizing thereof (Step 1); a step of adding 10 parts by weight to 20 parts by weight of the conjugated diene-based monomer and 0.1 part by weight to 1.0 part by weight of a second emulsifier simultaneously at the time point that the polymerization conversion rate of the polymerization reaction is 30% to 40% and then polymerizing thereof (Step 2); a step of adding a residual amount of a conjugated diene-based monomer and optionally 0 part by weight to 1 part by weight of a third emulsifier simultaneously or continuously at the time point that the polymerization conversion rate of the polymerization reaction is 60% to 70% and then polymerizing thereof (Step 3); and a step of completing polymerization by adding a polymerization inhibitor at the time point that the polymerization conversion rate of the polymerization reaction is 92% or higher (Step 4), which further comprises a step of additionally adding 0.05 part by weight to 0.3 part by weight of a cross-linking agent at the time point that the polymerization conversion rate of the polymerization reaction progressed through the step 1 and the step 2 is 0% to 50%, anda step of adding 0.01 part by weight to 0.5 part by weight of a fourth emulsifier having critical micelle concentration of 150 mg/L or less at the time point that the polymerization conversion rate of the polymerization reaction progressed through the step 1 and the step 2 is 50% to 85%. a step of adding 60 parts by weight to 75 parts by weight of a conjugated diene-based monomer, 1 part by weight to 3 parts by weight of a first emulsifier, 0.2 part by weight to 0.4 part by weight of a polymerization initiator, 0.2 part by weight to 3 parts by weight of an electrolyte, 0.1 part by weight to 0.5 part by weight of a molecular weight control agent and 65 parts by weight to 100 parts by weight of ion exchanged water into a reactor and then polymerizing thereof (Step 1); a step of adding 10 parts by weight to 20 parts by weight of the conjugated diene-based monomer and 0.1 part by weight to 1.0 part by weight of a second emulsifier simultaneously at the time point that the polymerization conversion rate of the polymerization reaction is 30% to 40% and then polymerizing thereof (Step 2); a step of adding a residual amount of a conjugated diene-based monomer and optionally 0 part by weight to 1 part by weight of a third emulsifier simultaneously or continuously at the time point that the polymerization conversion rate of the polymerization reaction is 60% to 70% and then polymerizing thereof (Step 3); and a step of completing polymerization by adding a polymerization inhibitor at the time point that the polymerization conversion rate of the polymerization reaction is 92% or higher (Step 4), which further comprises a step of additionally adding 0.05 part by weight to 0.3 part by weight of a cross-linking agent at the time point that the polymerization conversion rate of the polymerization reaction progressed through the step 1 and the step 2 is 0% to 50%, anda step of adding 0.01 part by weight to 0.5 part by weight of a fourth emulsifier having critical micelle concentration of 150 mg/L or less at the time point that the polymerization conversion rate of the polymerization reaction progressed through the step 1 and the step 2 is 50% to 85%.