A delivery assembly (20, 140) for delivering material into living tissue, the delivery assembly including: an access cannula (22, 142) that includes a tube formed from material capable of being inserted into a living body, the tube having opposed proximal and distal ends, the distal end of the tube configured for insertion into the living body and a lumen that extends through the tube; a delivery cannula the delivery cannula having a tube with opposed proximal and distal ends, the delivery cannula tube dimensioned to seat in the lumen of the access cannula tube and a fitting that leads to the distal end of the access cannula tube, the fitting dimensioned to receive a connector in communication with a container in which the material to be delivered into the living tissue is contained, characterized in that: a valve (48, 146) is attached to the access cannula adjacent the proximal end of the access cannula tube, said valve having at least one element (52, 150) that is normally in a closed position over the access cannula tube to prevent the proximal flow of material out of the tube and that is capable of moving to an open position upon the manual insertion of the delivery cannula tube in the access cannula (22, 142) and a biasing member that, upon removal to the delivery cannula tube from the access cannula tube, returns the at least one element to the closed position. an access cannula (22, 142) that includes a tube formed from material capable of being inserted into a living body, the tube having opposed proximal and distal ends, the distal end of the tube configured for insertion into the living body and a lumen that extends through the tube; a delivery cannula the delivery cannula having a tube with opposed proximal and distal ends, the delivery cannula tube dimensioned to seat in the lumen of the access cannula tube and a fitting that leads to the distal end of the access cannula tube, the fitting dimensioned to receive a connector in communication with a container in which the material to be delivered into the living tissue is contained, characterized in that: a valve (48, 146) is attached to the access cannula adjacent the proximal end of the access cannula tube, said valve having at least one element (52, 150) that is normally in a closed position over the access cannula tube to prevent the proximal flow of material out of the tube and that is capable of moving to an open position upon the manual insertion of the delivery cannula tube in the access cannula (22, 142) and a biasing member that, upon removal to the delivery cannula tube from the access cannula tube, returns the at least one element to the closed position. a valve (48, 146) is attached to the access cannula adjacent the proximal end of the access cannula tube, said valve having at least one element (52, 150) that is normally in a closed position over the access cannula tube to prevent the proximal flow of material out of the tube and that is capable of moving to an open position upon the manual insertion of the delivery cannula tube in the access cannula (22, 142) and a biasing member that, upon removal to the delivery cannula tube from the access cannula tube, returns the at least one element to the closed position.