Use of a vibrating diffuser in a fluorescence imaging system for performing pixel intensity measurements, said fluorescence imaging system comprising: a light source for producing a non-uniform fluence excitation illumination beam having a non-uniform beam fluence, said excitation illumination beam illuminating an object and configured for promoting fluorescence emissions; an optical system optically coupled with said light source and positioned between said light source and said object, said optical system configured to modify said non-uniform fluence illumination beam into a uniform fluence illumination beam having a uniform beam fluence, said optical system configured to change the divergence of said uniform fluence illumination beam; a camera having an array of pixels, said camera being configured for detecting said fluorescence emissions and for enabling said performance of pixel intensity measurements; an excitation light filter positioned between said object and said camera, said excitation light filter configured for filtering out said excitation illumination beam, such that said excitation illumination beam does not reach said camera; and a vibrating diffuser, for modifying said non-uniform fluence illumination beam into said uniform fluence illumination beam having said uniform beam fluence, wherein said optical system comprising an optical fiber configured to receive said non-uniform fluence illumination beam from said light source, said optical system being further configured to reimage an output of said optical fiber onto an intermediate image plane as said uniform fluence illumination beam, characterized in that said vibrating diffuser is located at said intermediate image plane; and said vibrating diffuser is configured to vibrate along two non-parallel axes in order to suppress inhomogeneities in said illumination beam caused by surface irregularities in said vibrating diffuser, thereby smoothing said non-uniform fluence excitation illumination beam into said uniform fluence illumination beam. a light source for producing a non-uniform fluence excitation illumination beam having a non-uniform beam fluence, said excitation illumination beam illuminating an object and configured for promoting fluorescence emissions; an optical system optically coupled with said light source and positioned between said light source and said object, said optical system configured to modify said non-uniform fluence illumination beam into a uniform fluence illumination beam having a uniform beam fluence, said optical system configured to change the divergence of said uniform fluence illumination beam; a camera having an array of pixels, said camera being configured for detecting said fluorescence emissions and for enabling said performance of pixel intensity measurements; an excitation light filter positioned between said object and said camera, said excitation light filter configured for filtering out said excitation illumination beam, such that said excitation illumination beam does not reach said camera; and a vibrating diffuser, for modifying said non-uniform fluence illumination beam into said uniform fluence illumination beam having said uniform beam fluence, wherein said optical system comprising an optical fiber configured to receive said non-uniform fluence illumination beam from said light source, said optical system being further configured to reimage an output of said optical fiber onto an intermediate image plane as said uniform fluence illumination beam, characterized in that said vibrating diffuser is located at said intermediate image plane; and said vibrating diffuser is configured to vibrate along two non-parallel axes in order to suppress inhomogeneities in said illumination beam caused by surface irregularities in said vibrating diffuser, thereby smoothing said non-uniform fluence excitation illumination beam into said uniform fluence illumination beam.