Method for amplifying and / or sequencing at least one RNA, preferably mRNA, contained in a sample, the method comprising: a) providing at least one first primer, which first primer includes a sequence which is complementary to a sequence which is located at or near the 3'-end of the at least one RNA to be amplified, and adding said at least one first primer to the sample under conditions which allow for hybridization of at least one first primer to at least one RNA, b) reverse transcribing the at least one RNA under conditions including addition of a reverse transcriptase and nucleotides to the sample to provide full length cDNA(s) of the at least one RNA, c) purifying the sample at least from excess nucleotides, d) adding a dideoxy nucleotide, which dideoxy nucleotide is modified at the 3'-position to include a first partner of a pair of azide and alkyne molecules, and a template-independent polymerase to attach a single 3'-azide- or 3'-alkyne modified dideoxy nucleotide at the 3'-end of the cDNA(s) obtained in step b), e) purifying the sample at least from excess modified dideoxy nucleotide added in step d), f) adding an adapter molecule, the adapter molecule comprising a polynucleotide sequence and, attached to its 5' end, a second partner of said pair of azide and alkyne molecules, under conditions to perform a click reaction and to ligate the adapter molecule to the 3'-modified cDNA(s) obtained in step d) under formation of a triazole linkage, g) adding a second primer, the second primer comprising a nucleotide sequence which is complementary to at least 6 of the nucleotides at the 5'-end of the adapter molecule and contains at its 3'-end a nucleotide which is complementary to the dideoxy nucleotide at the 3'-end of the cDNA(s), to effect hybridization and binding of the second primer to the ligated adapter/cDNA molecule obtained in step f) at a position overlapping the triazole linkage, and alternatively h) adding a DNA polymerase to achieve chain extension of the second primer to produce second strand DNA(s) including sequences complementary to the whole cDNA(s) obtained in step b), and either i) sequencing the second strand DNA(s) of step h), or j) adding a third primer which is identical to at least a part of the first primer, and amplifying the full length cDNA(s) to obtain a full length RNA library, or h') adding a DNA polymerase to achieve chain extension of the second primer and simultaneously determining the sequence of the second strand DNA(s) and the whole cDNA(s). a) providing at least one first primer, which first primer includes a sequence which is complementary to a sequence which is located at or near the 3'-end of the at least one RNA to be amplified, and adding said at least one first primer to the sample under conditions which allow for hybridization of at least one first primer to at least one RNA, b) reverse transcribing the at least one RNA under conditions including addition of a reverse transcriptase and nucleotides to the sample to provide full length cDNA(s) of the at least one RNA, c) purifying the sample at least from excess nucleotides, d) adding a dideoxy nucleotide, which dideoxy nucleotide is modified at the 3'-position to include a first partner of a pair of azide and alkyne molecules, and a template-independent polymerase to attach a single 3'-azide- or 3'-alkyne modified dideoxy nucleotide at the 3'-end of the cDNA(s) obtained in step b), e) purifying the sample at least from excess modified dideoxy nucleotide added in step d), f) adding an adapter molecule, the adapter molecule comprising a polynucleotide sequence and, attached to its 5' end, a second partner of said pair of azide and alkyne molecules, under conditions to perform a click reaction and to ligate the adapter molecule to the 3'-modified cDNA(s) obtained in step d) under formation of a triazole linkage, g) adding a second primer, the second primer comprising a nucleotide sequence which is complementary to at least 6 of the nucleotides at the 5'-end of the adapter molecule and contains at its 3'-end a nucleotide which is complementary to the dideoxy nucleotide at the 3'-end of the cDNA(s), to effect hybridization and binding of the second primer to the ligated adapter/cDNA molecule obtained in step f) at a position overlapping the triazole linkage, and alternatively h) adding a DNA polymerase to achieve chain extension of the second primer to produce second strand DNA(s) including sequences complementary to the whole cDNA(s) obtained in step b), and either i) sequencing the second strand DNA(s) of step h), or j) adding a third primer which is identical to at least a part of the first primer, and amplifying the full length cDNA(s) to obtain a full length RNA library, or h') adding a DNA polymerase to achieve chain extension of the second primer and simultaneously determining the sequence of the second strand DNA(s) and the whole cDNA(s).