A device for detecting an infection in a wound, comprising: a) a wound contacting layer; b) a reaction layer comprising one or more reagents that can indicate the presence of one or more analytes associated with an infection, wherein the reagents are affixed to a solid phase and produce a detectable signal in a reporter area and wherein the one or more reagents produce a visible color upon a change in the pH, at an acidic pH, or at a basic pH, wherein the pH-sensitive reagent comprises bromothymol blue, phenol red, bromophenol red, chlorophenol red, thymol blue, bromocresol green, bromocresol purple; nitrazine yellow; or other sulfophthalein dyes or wherein the reagents comprise enzyme-reactive regions that interact with one or more target enzymes selected from the group consisting of lysozyme, MPO, cathepsin G, elastase, catalase, lipase, esterase, and any combination thereof the enzyme-reactive regions comprise a moiety capable of producing a visible color or detectable electronic change upon interaction with one or more target enzymes, wherein the moiety is a peroxidase substrate, arylamine, an amino phenol, an indoxyl, a neutral dye, a charged dye, a nanoparticle, a colloidal gold particle, or an analog thereof and the reagent interacts with a target enzyme to produce a colored species or to produce an intermediate product that interacts with an accessory enzyme selected from the group consisting of a lipase, esterase, hexosaminidase, peroxidase, oxidase, glycosidase, glucosidase, and laccase; c) a cover on top of the reaction layer, wherein the cover comprises one or more windows or clear areas to allow visualization of the detectable signal; and d) fluid communication between the wound contacting layer and the reaction layer, wherein the fluid communication comprises wicking stitching or wicking tufting of an absorbent material that allows fluid communication between the wound contacting layer and the reaction layer and wherein the wicking stitching or wicking tufting comprises cotton, rayon, viscose, wool, silk, polyester, polyamide, and CMC fibers, solid or hollow fibers. a) a wound contacting layer; b) a reaction layer comprising one or more reagents that can indicate the presence of one or more analytes associated with an infection, wherein the reagents are affixed to a solid phase and produce a detectable signal in a reporter area and wherein the one or more reagents produce a visible color upon a change in the pH, at an acidic pH, or at a basic pH, wherein the pH-sensitive reagent comprises bromothymol blue, phenol red, bromophenol red, chlorophenol red, thymol blue, bromocresol green, bromocresol purple; nitrazine yellow; or other sulfophthalein dyes or wherein the reagents comprise enzyme-reactive regions that interact with one or more target enzymes selected from the group consisting of lysozyme, MPO, cathepsin G, elastase, catalase, lipase, esterase, and any combination thereof the enzyme-reactive regions comprise a moiety capable of producing a visible color or detectable electronic change upon interaction with one or more target enzymes, wherein the moiety is a peroxidase substrate, arylamine, an amino phenol, an indoxyl, a neutral dye, a charged dye, a nanoparticle, a colloidal gold particle, or an analog thereof and the reagent interacts with a target enzyme to produce a colored species or to produce an intermediate product that interacts with an accessory enzyme selected from the group consisting of a lipase, esterase, hexosaminidase, peroxidase, oxidase, glycosidase, glucosidase, and laccase; c) a cover on top of the reaction layer, wherein the cover comprises one or more windows or clear areas to allow visualization of the detectable signal; and d) fluid communication between the wound contacting layer and the reaction layer, wherein the fluid communication comprises wicking stitching or wicking tufting of an absorbent material that allows fluid communication between the wound contacting layer and the reaction layer and wherein the wicking stitching or wicking tufting comprises cotton, rayon, viscose, wool, silk, polyester, polyamide, and CMC fibers, solid or hollow fibers.