An information processing apparatus, comprising: an image acquisition section that acquires image information of a specimen stained by a fluorescence reagent; an information acquisition section that acquires measurement channel indicative of a first autofluorescent component and a second autofluorescent component included in the specimen and spectral information regarding an autofluorescent spectrum possessed by each of the first autofluorescent component and the second autofluorescent component included in the specimen and spectral information regarding a fluorescence spectrum possessed by fluorescent components included in the fluorescence reagent; and an analysis section that separates a first fluorescence signal from the image information in accordance with the measurement channel and spectral information regarding the specimen and separates a second fluorescence signal from the image information in accordance with the spectral information regarding the fluorescent components included in the fluorescence reagent, wherein the first fluorescence signal includes an autofluorescence signal of the first autofluorescent component and an autofluorescence signal of the second autofluorescent component, wherein the spectral information of each of the autofluorescent components varies depending on immobilization and wherein the second fluorescence signal includes a fluorescence signal of the fluorescence reagent, performs segmentation, based on the separated first and second fluorescence signals, for recognising a region of an object or tissue included in the image information, and analyzes a fixation state of the specimen in accordance with a component ratio of each of the autofluorescence signal of the first autofluorescent component and the autofluorescence signal of the second autofluorescent component. an image acquisition section that acquires image information of a specimen stained by a fluorescence reagent; an information acquisition section that acquires measurement channel indicative of a first autofluorescent component and a second autofluorescent component included in the specimen and spectral information regarding an autofluorescent spectrum possessed by each of the first autofluorescent component and the second autofluorescent component included in the specimen and spectral information regarding a fluorescence spectrum possessed by fluorescent components included in the fluorescence reagent; and an analysis section that separates a first fluorescence signal from the image information in accordance with the measurement channel and spectral information regarding the specimen and separates a second fluorescence signal from the image information in accordance with the spectral information regarding the fluorescent components included in the fluorescence reagent, wherein the first fluorescence signal includes an autofluorescence signal of the first autofluorescent component and an autofluorescence signal of the second autofluorescent component, wherein the spectral information of each of the autofluorescent components varies depending on immobilization and wherein the second fluorescence signal includes a fluorescence signal of the fluorescence reagent, performs segmentation, based on the separated first and second fluorescence signals, for recognising a region of an object or tissue included in the image information, and analyzes a fixation state of the specimen in accordance with a component ratio of each of the autofluorescence signal of the first autofluorescent component and the autofluorescence signal of the second autofluorescent component. separates a first fluorescence signal from the image information in accordance with the measurement channel and spectral information regarding the specimen and separates a second fluorescence signal from the image information in accordance with the spectral information regarding the fluorescent components included in the fluorescence reagent, wherein the first fluorescence signal includes an autofluorescence signal of the first autofluorescent component and an autofluorescence signal of the second autofluorescent component, wherein the spectral information of each of the autofluorescent components varies depending on immobilization and wherein the second fluorescence signal includes a fluorescence signal of the fluorescence reagent, performs segmentation, based on the separated first and second fluorescence signals, for recognising a region of an object or tissue included in the image information, and analyzes a fixation state of the specimen in accordance with a component ratio of each of the autofluorescence signal of the first autofluorescent component and the autofluorescence signal of the second autofluorescent component.