A microfluidic apparatus mounted on a rotation driving unit, the microfluidic apparatus characterized by: a sample chamber extending in a radial direction from a rotation center of the microfluidic apparatus and adapted to receive a biological sample and separate the biological sample into a first fluid layer comprising a first target material and a second fluid layer comprising a second target material using centrifugal force; a first sample channel connected to the sample chamber at a first location on the sample chamber from the rotation center in the radial direction; a second sample channel connected to the sample chamber at a second location on the sample chamber from the rotation center in the radial direction; a first enriching unit connected to the sample chamber by the first sample channel and containing a first fine particle that specifically binds to the first target material, wherein the first enriching unit receives the first fluid layer from the sample chamber via the first connection channel to form a first complex of the first fine particle and the first target material, and separates the first complex from the first fluid layer using a density difference with a density-gradient material having a lower density than the first complex; and a second enriching unit connected to the sample chamber by the second sample channel and containing a second fine particle that specifically binds to the second target material, wherein the second enriching unit receives the second fluid layer from the sample chamber to form a second complex of the second fine particle and the second target material, and separate the second complex from the second fluid layer using a density difference between the second fluid layer and the second complex, wherein the first enriching unit comprises: a first particle chamber that houses the first fine particle and connected to the sample chamber by the first sample channel to receive the first fluid layer from the sample chamber; a separation chamber that houses the DGM and connected to the first particle chamber by a separation channel to receive the first fluid layer including the first complex; and a first recovering chamber connected to the separation chamber by a first recovering channel to receive the first complex separated from the first fluid layer. a sample chamber extending in a radial direction from a rotation center of the microfluidic apparatus and adapted to receive a biological sample and separate the biological sample into a first fluid layer comprising a first target material and a second fluid layer comprising a second target material using centrifugal force; a first sample channel connected to the sample chamber at a first location on the sample chamber from the rotation center in the radial direction; a second sample channel connected to the sample chamber at a second location on the sample chamber from the rotation center in the radial direction; a first enriching unit connected to the sample chamber by the first sample channel and containing a first fine particle that specifically binds to the first target material, wherein the first enriching unit receives the first fluid layer from the sample chamber via the first connection channel to form a first complex of the first fine particle and the first target material, and separates the first complex from the first fluid layer using a density difference with a density-gradient material having a lower density than the first complex; and a second enriching unit connected to the sample chamber by the second sample channel and containing a second fine particle that specifically binds to the second target material, wherein the second enriching unit receives the second fluid layer from the sample chamber to form a second complex of the second fine particle and the second target material, and separate the second complex from the second fluid layer using a density difference between the second fluid layer and the second complex, wherein the first enriching unit comprises: a first particle chamber that houses the first fine particle and connected to the sample chamber by the first sample channel to receive the first fluid layer from the sample chamber; a separation chamber that houses the DGM and connected to the first particle chamber by a separation channel to receive the first fluid layer including the first complex; and a first recovering chamber connected to the separation chamber by a first recovering channel to receive the first complex separated from the first fluid layer. a first particle chamber that houses the first fine particle and connected to the sample chamber by the first sample channel to receive the first fluid layer from the sample chamber; a separation chamber that houses the DGM and connected to the first particle chamber by a separation channel to receive the first fluid layer including the first complex; and a first recovering chamber connected to the separation chamber by a first recovering channel to receive the first complex separated from the first fluid layer.