A method of manufacturing an electrophoretic display module with double-layer microstructure, comprising the following steps: step 1: preparing a plasma barrier array on a pixel electrode in advance; step 2: embedding the pixel electrode in a TFT glass substrate, and placing the TFT glass substrate on a dispensing platform; step 3: applying a sealing adhesive on the pixel electrode to form a liner frame which surrounds the pixel electrode; step 4: silk screening a display plasma comprising electrophoretic particles and spraying spacer particles within the space surrounded by the liner frame, wherein the relatively light spacer particles float on the display plasma; step 5: coating a conductive silver paste in the liner frame, wherein the conductive silver paste is electrically connected to the pixel electrode; step 6: firstly, laminating a display area protective layer on the whole liner frame, secondly, laminating a transparent electrode on the liner frame and the display plasma, wherein, in the process, most of the spacer particles are squeezed, transferred, and adsorbed on the plasma barrier frame of the plasma barrier array to form the spacer particle layer, and then solidifying; step 7: cutting off edges of the transparent electrode and the display area protective layer to expose a position predetermined to bind an IC integrated circuit module and a flexible circuit board on the pixel electrode step 8: adhering the IC integrated circuit module and the flexible circuit board to an edge of the pixel electrode through a conductive adhesive tape; and step 9: firmly sealing peripheries of the IC integrated circuit module, the flexible circuit board and the conductive adhesive tape on the pixel electrode by a blue adhesive, thus completing manufacturing of the electronic ink display screen. step 1: preparing a plasma barrier array on a pixel electrode in advance; step 2: embedding the pixel electrode in a TFT glass substrate, and placing the TFT glass substrate on a dispensing platform; step 3: applying a sealing adhesive on the pixel electrode to form a liner frame which surrounds the pixel electrode; step 4: silk screening a display plasma comprising electrophoretic particles and spraying spacer particles within the space surrounded by the liner frame, wherein the relatively light spacer particles float on the display plasma; step 5: coating a conductive silver paste in the liner frame, wherein the conductive silver paste is electrically connected to the pixel electrode; step 6: firstly, laminating a display area protective layer on the whole liner frame, secondly, laminating a transparent electrode on the liner frame and the display plasma, wherein, in the process, most of the spacer particles are squeezed, transferred, and adsorbed on the plasma barrier frame of the plasma barrier array to form the spacer particle layer, and then solidifying; step 7: cutting off edges of the transparent electrode and the display area protective layer to expose a position predetermined to bind an IC integrated circuit module and a flexible circuit board on the pixel electrode step 8: adhering the IC integrated circuit module and the flexible circuit board to an edge of the pixel electrode through a conductive adhesive tape; and step 9: firmly sealing peripheries of the IC integrated circuit module, the flexible circuit board and the conductive adhesive tape on the pixel electrode by a blue adhesive, thus completing manufacturing of the electronic ink display screen.