A microfluidic device for handling a fluid sample comprising: at least one microfluidic well configured to receive the fluid sample, wherein the at least one microfluidic well includes a plurality of microfluidic channels, each microfluidic channel fluidly couples a group of microchambers and wherein each microfluidic channel fluidly couples the group of microchambers in series; the microfluidic well further comprising a primary inlet fluidly coupled to an inlet microfluidic channel, the inlet microfluidic channel fluidly coupled via an inlet end to the plurality of microfluidic channels arranged in parallel that are fluidly coupled via an outlet end to an outlet microfluidic channel, the outlet microfluidic channel fluidly coupled to a primary vent configured to vent gas from the plurality of microchambers, each microchamber including a reaction chamber and a vent chamber, the reaction chamber configured to receive the fluid sample from the microfluidic channel and fluidly coupled to the vent chamber configured to vent gas from the reaction chamber via the microfluidic channel as the fluid sample flows into the reaction chamber, wherein the vent chamber is configured to initially prevent flow of the fluid sample from the reaction chamber while allowing flow of gas from the reaction chamber as the fluid sample flows into the reaction chamber, and wherein the reaction chamber is fluidly coupled to a first segment of the microfluidic channel and the vent chamber is fluidly coupled to a second segment of the microfluidic channel, and the first segment of the microfluidic channel fluidly couples the reaction chamber to a first microchamber located adjacent to the reaction chamber, and the second segment of the microfluidic channel fluidly couples the vent chamber to a second microchamber located adjacent to the vent chamber. at least one microfluidic well configured to receive the fluid sample, wherein the at least one microfluidic well includes a plurality of microfluidic channels, each microfluidic channel fluidly couples a group of microchambers and wherein each microfluidic channel fluidly couples the group of microchambers in series; the microfluidic well further comprising a primary inlet fluidly coupled to an inlet microfluidic channel, the inlet microfluidic channel fluidly coupled via an inlet end to the plurality of microfluidic channels arranged in parallel that are fluidly coupled via an outlet end to an outlet microfluidic channel, a primary inlet fluidly coupled to an inlet microfluidic channel, the inlet microfluidic channel fluidly coupled via an inlet end to the plurality of microfluidic channels arranged in parallel that are fluidly coupled via an outlet end to an outlet microfluidic channel, the outlet microfluidic channel fluidly coupled to a primary vent configured to vent gas from the plurality of microchambers, each microchamber including a reaction chamber and a vent chamber, the reaction chamber configured to receive the fluid sample from the microfluidic channel and fluidly coupled to the vent chamber configured to vent gas from the reaction chamber via the microfluidic channel as the fluid sample flows into the reaction chamber, wherein the vent chamber is configured to initially prevent flow of the fluid sample from the reaction chamber while allowing flow of gas from the reaction chamber as the fluid sample flows into the reaction chamber, and wherein the reaction chamber is fluidly coupled to a first segment of the microfluidic channel and the vent chamber is fluidly coupled to a second segment of the microfluidic channel, and the first segment of the microfluidic channel fluidly couples the reaction chamber to a first microchamber located adjacent to the reaction chamber, and the second segment of the microfluidic channel fluidly couples the vent chamber to a second microchamber located adjacent to the vent chamber.