A method of analysis for quantifying a nucleic acid in a sample, the method comprising: segregating a sample comprising a target nucleic acid into a plurality of sample reaction volumes, wherein the plurality of sample reaction volumes include a first plurality of the sample reaction volumes each containing at least one molecule of the target nucleic acid and a second plurality of the sample reaction volumes each containing no molecules of the target nucleic acid; subjecting the plurality of sample reaction volumes to a digital amplification assay, wherein the digital amplification assay is configured to amplify the target nucleic acid to produce an amplified product; after the subjecting, and at a first temperature of the sample reaction volumes, taking a set of first measurements of an indicator of amplification presented by the target nucleic acid for respective sample reaction volumes of the plurality of the sample reaction volumes; detecting and/or measuring an amount of the amplified product for each sample reaction volume based on the set of first measurements; and after taking the set of first measurements, taking at least one post-amplification measurement of the indicator of amplification for at least some of the plurality of sample reaction volumes, wherein, when a post-amplification temperature is higher than the first temperature, taking a set of additional measurements further comprises: identifying one or more changes in the indicator of amplification based on the set of additional measurements; associating the at least one change to a post-amplification temperature; comparing the post-amplification temperature associated with the at least one change to an expected melt temperature for the target nucleic acid; identifying erroneous amplification product based on the comparing, and quantifying the target nucleic acid in the sample based on the set of additional measurements and the identified erroneous amplification product. segregating a sample comprising a target nucleic acid into a plurality of sample reaction volumes, wherein the plurality of sample reaction volumes include a first plurality of the sample reaction volumes each containing at least one molecule of the target nucleic acid and a second plurality of the sample reaction volumes each containing no molecules of the target nucleic acid; subjecting the plurality of sample reaction volumes to a digital amplification assay, wherein the digital amplification assay is configured to amplify the target nucleic acid to produce an amplified product; after the subjecting, and at a first temperature of the sample reaction volumes, taking a set of first measurements of an indicator of amplification presented by the target nucleic acid for respective sample reaction volumes of the plurality of the sample reaction volumes; detecting and/or measuring an amount of the amplified product for each sample reaction volume based on the set of first measurements; and after taking the set of first measurements, taking at least one post-amplification measurement of the indicator of amplification for at least some of the plurality of sample reaction volumes, wherein, when a post-amplification temperature is higher than the first temperature, taking a set of additional measurements further comprises: identifying one or more changes in the indicator of amplification based on the set of additional measurements; associating the at least one change to a post-amplification temperature; comparing the post-amplification temperature associated with the at least one change to an expected melt temperature for the target nucleic acid; identifying erroneous amplification product based on the comparing, and quantifying the target nucleic acid in the sample based on the set of additional measurements and the identified erroneous amplification product. identifying one or more changes in the indicator of amplification based on the set of additional measurements; associating the at least one change to a post-amplification temperature; comparing the post-amplification temperature associated with the at least one change to an expected melt temperature for the target nucleic acid; identifying erroneous amplification product based on the comparing, and quantifying the target nucleic acid in the sample based on the set of additional measurements and the identified erroneous amplification product.