A method of measuring a fluorescence signal in a tissue of a limb, to which a fluorescent agent has been added, and of determining a 3D representation of at least a section of the limb, wherein the tissue to which the fluorescent agent has been added forms part of the limb, the method comprising the steps of: - capturing a fluorescence image by illuminating the tissue with excitation light having a wavelength suitable to generate emitted light by excited emission of the fluorescent agent, and by spatially resolved measurement of the emitted light so as to provide the fluorescence image, - capturing data on a topology of the surface of at least the section of the limb and determining a 3D representation of at least the section of the limb from the captured data, - capturing a visible light image of at least the section of the surface of the limb, wherein a viewing direction and/or a perspective of the fluorescence image and the visible light image are linked via a known relationship, - repeating the steps of capturing of the fluorescence image and capturing of the visible light image to provide a series of fluorescence images and a series of visible light images, wherein the capturing of data on the topology of the surface of the limb is performed in at least the section of the limb that is imaged when capturing the series of fluorescence images and the visible light images, - applying a stitching algorithm on the series of visible light images to generate a large visible light image, wherein the stitching algorithm determines and applies a set of stitching parameters, - applying the stitching algorithm on the series of fluorescence images to generate a large fluorescence image, wherein the stitching algorithm applies the set of stitching parameters determined when performing the stitching of the visible light images, if the viewing direction and perspective of the visible light images and the fluorescence images are identical, and the stitching algorithm applies the set of stitching parameters having a fixed offset, if the viewing direction and perspective of the visible light images and the fluorescence images are not identical, - outputting the large visible light image together with the large fluorescence image and the visualization of the 3D representation. - capturing a fluorescence image by illuminating the tissue with excitation light having a wavelength suitable to generate emitted light by excited emission of the fluorescent agent, and by spatially resolved measurement of the emitted light so as to provide the fluorescence image, - capturing data on a topology of the surface of at least the section of the limb and determining a 3D representation of at least the section of the limb from the captured data, - capturing a visible light image of at least the section of the surface of the limb, wherein a viewing direction and/or a perspective of the fluorescence image and the visible light image are linked via a known relationship, - repeating the steps of capturing of the fluorescence image and capturing of the visible light image to provide a series of fluorescence images and a series of visible light images, wherein the capturing of data on the topology of the surface of the limb is performed in at least the section of the limb that is imaged when capturing the series of fluorescence images and the visible light images, - applying a stitching algorithm on the series of visible light images to generate a large visible light image, wherein the stitching algorithm determines and applies a set of stitching parameters, - applying the stitching algorithm on the series of fluorescence images to generate a large fluorescence image, wherein the stitching algorithm applies the set of stitching parameters determined when performing the stitching of the visible light images, if the viewing direction and perspective of the visible light images and the fluorescence images are identical, and the stitching algorithm applies the set of stitching parameters having a fixed offset, if the viewing direction and perspective of the visible light images and the fluorescence images are not identical, the stitching algorithm applies the set of stitching parameters determined when performing the stitching of the visible light images, if the viewing direction and perspective of the visible light images and the fluorescence images are identical, and the stitching algorithm applies the set of stitching parameters having a fixed offset, if the viewing direction and perspective of the visible light images and the fluorescence images are not identical, - outputting the large visible light image together with the large fluorescence image and the visualization of the 3D representation.