An optical imaging equipment, comprising: an optical microscope, comprising an objective table, an objective lens and a light source module, wherein the objective table is movable in a x-y plane, the light source module comprises light sources for providing illumination light for optical imaging, the objective lens is movable in a z-axis direction which is perpendicular to the x-y plane; a 3D electric sample table, fixed on the objective table for carrying a sample to be tested and driving the sample to be tested to move in 3D directions relative to the objective table; and a transparent substrate and a microsphere fixed on the transparent substrate, wherein there exists only one microsphere, wherein the objective lens, the microsphere, and the sample to be tested are arranged along the z-axis direction in sequence; wherein the transparent substrate along with the microsphere thereon is configured to be moved to a first position and remain stationary with respect to the objective table in the z-axis direction; the 3D electric sample table is configured to adjust the sample to be tested with respect to the microsphere to an imaging plane which is parallel to the x-y plane and a first image is formed by the microsphere; the objective lens is configured to be adjusted to a second position, so that the objective lens is configured to perform a secondary imaging to the first image to form a second image, wherein the 3D electric sample table comprises a fixed base and a moving base, the fixed base being fixed on the objective table, wherein the transparent substrate is directly mounted on a top surface of the fixed base, and the microsphere is fixed on a lower surface of the transparent substrate and mounted in a downward direction facing away from the objective lens. an optical microscope, comprising an objective table, an objective lens and a light source module, wherein the objective table is movable in a x-y plane, the light source module comprises light sources for providing illumination light for optical imaging, the objective lens is movable in a z-axis direction which is perpendicular to the x-y plane; a 3D electric sample table, fixed on the objective table for carrying a sample to be tested and driving the sample to be tested to move in 3D directions relative to the objective table; and a transparent substrate and a microsphere fixed on the transparent substrate, wherein there exists only one microsphere, wherein the objective lens, the microsphere, and the sample to be tested are arranged along the z-axis direction in sequence; wherein the transparent substrate along with the microsphere thereon is configured to be moved to a first position and remain stationary with respect to the objective table in the z-axis direction; the 3D electric sample table is configured to adjust the sample to be tested with respect to the microsphere to an imaging plane which is parallel to the x-y plane and a first image is formed by the microsphere; the objective lens is configured to be adjusted to a second position, so that the objective lens is configured to perform a secondary imaging to the first image to form a second image, wherein the 3D electric sample table comprises a fixed base and a moving base, the fixed base being fixed on the objective table, wherein the transparent substrate is directly mounted on a top surface of the fixed base, and the microsphere is fixed on a lower surface of the transparent substrate and mounted in a downward direction facing away from the objective lens.