An apparatus for filling vials having a chamber and an inlet tube with therapeutic biological liquids, comprising: a carriage for holding a plurality of the vials through a filling and sealing process, wherein the carriage includes a tray as a base; a liquid nozzle connected to a liquid dispenser that draws from a liquid reservoir, the liquid nozzle lowerable into inlet tubes of individual vials to dispense liquid via the inlet tube into the vial chamber so that some liquid remains in the inlet tube; characterised in that: the tray has side upwardly-extending walls; the apparatus further comprises an air nozzle connected to an air dispenser and dispensing filtered air, the air nozzle lowerable into inlet tubes of individual vials and operable to force air into the inlet tube to force liquid through the inlet tube into the vial chamber and to create suction to remove a portion of air from the inlet tube so as to leave an aliquot of liquid between air pockets in the inlet tube; and the apparatus further comprises at least one pair of sealing bars, each having a pair of heating elements separated by at least the distance between the air pockets, adapted to be pressed together against the inlet tube to make seals in the inlet tube on either side of the aliquot in the inlet tube. a carriage for holding a plurality of the vials through a filling and sealing process, wherein the carriage includes a tray as a base; a liquid nozzle connected to a liquid dispenser that draws from a liquid reservoir, the liquid nozzle lowerable into inlet tubes of individual vials to dispense liquid via the inlet tube into the vial chamber so that some liquid remains in the inlet tube; characterised in that: the tray has side upwardly-extending walls; the apparatus further comprises an air nozzle connected to an air dispenser and dispensing filtered air, the air nozzle lowerable into inlet tubes of individual vials and operable to force air into the inlet tube to force liquid through the inlet tube into the vial chamber and to create suction to remove a portion of air from the inlet tube so as to leave an aliquot of liquid between air pockets in the inlet tube; and the apparatus further comprises at least one pair of sealing bars, each having a pair of heating elements separated by at least the distance between the air pockets, adapted to be pressed together against the inlet tube to make seals in the inlet tube on either side of the aliquot in the inlet tube. the tray has side upwardly-extending walls; the apparatus further comprises an air nozzle connected to an air dispenser and dispensing filtered air, the air nozzle lowerable into inlet tubes of individual vials and operable to force air into the inlet tube to force liquid through the inlet tube into the vial chamber and to create suction to remove a portion of air from the inlet tube so as to leave an aliquot of liquid between air pockets in the inlet tube; and the apparatus further comprises at least one pair of sealing bars, each having a pair of heating elements separated by at least the distance between the air pockets, adapted to be pressed together against the inlet tube to make seals in the inlet tube on either side of the aliquot in the inlet tube.