A method for the reconstruction of a tissue-engineered human corneal endothelium, comprising the steps, wherein: first of all, human corneal endothelial cells are cultured and proliferated in 20% calf bovine serum-containing DMEM/F12 medium; after digestion with 0.25% trypsin, human corneal endothelial cells are harvested with centrifugation at 270-300 g for 10-15 minutes; the cells are then re-suspended in a specific medium (DMEM/F12 medium supplemented with 20% calf bovine serum and 0.05%-0.1% type-IV collagen) in order to produce human corneal endothelial cell suspension; the freeze-dried human amniotic membrane is placed up-side down on a Petri dish with its epithelial layer underneath; the epithelial layer of the amniotic membrane is digested with 0.25% trypsin solution using an "inverted digestion method" in order to produce denuded human amniotic membrane, which is subsequently punched into discs in the same diameter as culture plate wells with a puncher; finally, denuded amniotic membrane discs are tiled onto the bottom of wells of a 48-well cell culture plate with their epithelial layer on top; the plate is kept in a 37°C incubator till discs are dried and completely adhered to the bottom, which are therefore used as amniotic membrane scaffold carriers; a human corneal endothelial cell suspension is inoculated into the culture plate tiled with above amniotic membrane scaffolds and inoculation amount varies between 0.1mL and 0.2mL; when cells completely adhered to scaffold carriers, additional specific medium mentioned above is added to lift liquid surface of culture medium to 3/4 wells height; when human corneal endothelial cells expand to form a single layer, a tissue-engineered human corneal endothelium is eventually reconstructed. first of all, human corneal endothelial cells are cultured and proliferated in 20% calf bovine serum-containing DMEM/F12 medium; after digestion with 0.25% trypsin, human corneal endothelial cells are harvested with centrifugation at 270-300 g for 10-15 minutes; the cells are then re-suspended in a specific medium (DMEM/F12 medium supplemented with 20% calf bovine serum and 0.05%-0.1% type-IV collagen) in order to produce human corneal endothelial cell suspension; the freeze-dried human amniotic membrane is placed up-side down on a Petri dish with its epithelial layer underneath; the epithelial layer of the amniotic membrane is digested with 0.25% trypsin solution using an "inverted digestion method" in order to produce denuded human amniotic membrane, which is subsequently punched into discs in the same diameter as culture plate wells with a puncher; finally, denuded amniotic membrane discs are tiled onto the bottom of wells of a 48-well cell culture plate with their epithelial layer on top; the plate is kept in a 37°C incubator till discs are dried and completely adhered to the bottom, which are therefore used as amniotic membrane scaffold carriers; a human corneal endothelial cell suspension is inoculated into the culture plate tiled with above amniotic membrane scaffolds and inoculation amount varies between 0.1mL and 0.2mL; when cells completely adhered to scaffold carriers, additional specific medium mentioned above is added to lift liquid surface of culture medium to 3/4 wells height; when human corneal endothelial cells expand to form a single layer, a tissue-engineered human corneal endothelium is eventually reconstructed.