A reference cassette for a fluorescence immunoassay diagnostic apparatus which is insertable into the apparatus in order to diagnose a disease from a biopsy specimen using fluorescence and configured to generate fluorescence by a test light of an optical module, the reference cassette for the fluorescence immunoassay diagnostic apparatus comprising: a case configured to be insertable into the fluorescence immunoassay diagnostic apparatus, wherein an opening is formed at least a part of an upper side of the case so that an inner space of the case is exposed to an outside of the case; and a fluorescence glass disposed at the inner space of the case so that the fluorescence glass is exposed to the outside of the case through the opening of the case, wherein the fluorescence glass is configured to generate the fluorescence by the test light of the optical module and constantly maintain fluorescence characteristics, wherein a mask is disposed on an upper surface of the fluorescence glass inside the case so that an area of the fluorescence glass exposed to the outside of the case is limited to a specific area of the fluorescence glass, wherein the mask has one or more slits and the fluorescence glass is exposed through the slits, wherein a separate fixing guide having a plurality of insertion coupling holes is coupled to an inside of the case, the fluorescence glass includes a plurality of pieces of the fluorescence glass, each piece of the fluorescence glass is insertedly coupled to a respective insertion coupling hole of the fixing guide, and the mask is coupled to the fixing guide so that each of the slits limits an outside exposure area of the fluorescence glass to a slit area, and wherein an upper surface of the fluorescence glass and an upper surface of the fixing guide are in a same plane as each other in a state that the fluorescence glass is insertedly coupled to the insertion coupling holes of the fixing guide, and the mask is coupled to the fixing guide to contact the upper surface of the fluorescence glass and the upper surface of the fixing guide. a case configured to be insertable into the fluorescence immunoassay diagnostic apparatus, wherein an opening is formed at least a part of an upper side of the case so that an inner space of the case is exposed to an outside of the case; and a fluorescence glass disposed at the inner space of the case so that the fluorescence glass is exposed to the outside of the case through the opening of the case, wherein the fluorescence glass is configured to generate the fluorescence by the test light of the optical module and constantly maintain fluorescence characteristics, wherein a mask is disposed on an upper surface of the fluorescence glass inside the case so that an area of the fluorescence glass exposed to the outside of the case is limited to a specific area of the fluorescence glass, wherein the mask has one or more slits and the fluorescence glass is exposed through the slits, wherein a separate fixing guide having a plurality of insertion coupling holes is coupled to an inside of the case, the fluorescence glass includes a plurality of pieces of the fluorescence glass, each piece of the fluorescence glass is insertedly coupled to a respective insertion coupling hole of the fixing guide, and the mask is coupled to the fixing guide so that each of the slits limits an outside exposure area of the fluorescence glass to a slit area, and wherein an upper surface of the fluorescence glass and an upper surface of the fixing guide are in a same plane as each other in a state that the fluorescence glass is insertedly coupled to the insertion coupling holes of the fixing guide, and the mask is coupled to the fixing guide to contact the upper surface of the fluorescence glass and the upper surface of the fixing guide.