Method for correcting a Raman spectrum produced by a sample, comprising the following steps: a) illuminating a surface of the sample with a first light beam produced by a first light source, said first light source being a laser source coupled to a first optical system, able to focus said first light beam on an object focal plane (5.1) of said first optical system, said object focal plane being located, in the sample, at a depth z, called the measurement depth, from the surface of the sample, b) measuring, using a first photodetector, a first optical signal backscattered by the sample in response to said first light beam, the first photodetector being a Raman spectrophotometer that produces a Raman spectrum SP z representative of said first optical signal, a spatial filter being interposed between said first optical system and the first photodetector, said spatial filter comprising a window (6.1) able to transmit said first optical signal towards said first photodetector, said window (6.1) being placed in a conjugate focal plane (5.2) of said object focal plane (5.1) of said first optical system, characterized in that the method also comprises the following steps: c) determining a reduced scattering coefficient μs' or a scattering coefficient μs of said sample; d) determining a correction term β, depending on the reduced scattering coefficient or the scattering coefficient, e) taking into account a threshold depth zt, from which the measured spectrum no longer significantly varies; f) measuring, using the first photodetector a Raman depth spectrum SP depth representative of the first optical signal backscattered by the sample in response to the first light beam, when the first object focal plane (5.1) is located, in the sample, at a depth z depth from the surface, said depth z depth being larger than the threshold depth z t, g) applying a correction function Fcorr to the Raman spectrum SP z so as to generate a corrected first signal SP corr-z, the said correction function taking into account the Raman depth spectrum SP depth and the correction term β. a) illuminating a surface of the sample with a first light beam produced by a first light source, said first light source being a laser source coupled to a first optical system, able to focus said first light beam on an object focal plane (5.1) of said first optical system, said object focal plane being located, in the sample, at a depth z, called the measurement depth, from the surface of the sample, b) measuring, using a first photodetector, a first optical signal backscattered by the sample in response to said first light beam, the first photodetector being a Raman spectrophotometer that produces a Raman spectrum SP z representative of said first optical signal, a spatial filter being interposed between said first optical system and the first photodetector, said spatial filter comprising a window (6.1) able to transmit said first optical signal towards said first photodetector, said window (6.1) being placed in a conjugate focal plane (5.2) of said object focal plane (5.1) of said first optical system, c) determining a reduced scattering coefficient μs' or a scattering coefficient μs of said sample; d) determining a correction term β, depending on the reduced scattering coefficient or the scattering coefficient, e) taking into account a threshold depth zt, from which the measured spectrum no longer significantly varies; f) measuring, using the first photodetector a Raman depth spectrum SP depth representative of the first optical signal backscattered by the sample in response to the first light beam, when the first object focal plane (5.1) is located, in the sample, at a depth z depth from the surface, said depth z depth being larger than the threshold depth z t, g) applying a correction function Fcorr to the Raman spectrum SP z so as to generate a corrected first signal SP corr-z, the said correction function taking into account the Raman depth spectrum SP depth and the correction term β.