mhurhangee/ep-ind-claims huggingface dataset:
A method of identifying a target protein specific translational fusion partner capable of inducing secretory production of a recombinant target protein that otherwise is not produced or is produced at a low level, said method comprising: (i) co-transforming a plurality of reporter protein deficient host cells with a plurality of linear vectors and a nucleic acid encoding a target protein to produce a plurality of transformed host cells, wherein each of said linear vectors comprises a nucleic acid fragment which is a candidate TFP from a library of nucleic acid fragments and a nucleotide sequence encoding a N-terminal amino acid-deleted reporter protein, and wherein said nucleic acid encoding a target protein comprises, at the 3' end, a nucleotide sequence encoding the N-terminal amino acids deleted from said reporter protein in said linear vector, and at the 5' end, a linker DNA; (ii) incubating said plurality of transformed host cells under conditions effective to allow in vivo recombination of said linear vectors and said nucleic acid encoding a target protein, whereby the nucleic acid encoding the target protein is inserted in frame between the nucleic acid fragment and the nucleotide sequence encoding the reporter protein; (iii) identifying a cell showing secretion of the reporter protein activity from the plurality of transformed host cells of (ii); and (iv) identifying as a TFP a nucleic acid fragment which induces the secretion of said target protein from the cell identified in (iii). (i) co-transforming a plurality of reporter protein deficient host cells with a plurality of linear vectors and a nucleic acid encoding a target protein to produce a plurality of transformed host cells, (ii) incubating said plurality of transformed host cells under conditions effective to allow in vivo recombination of said linear vectors and said nucleic acid encoding a target protein, whereby the nucleic acid encoding the target protein is inserted in frame between the nucleic acid fragment and the nucleotide sequence encoding the reporter protein; (iii) identifying a cell showing secretion of the reporter protein activity from the plurality of transformed host cells of (ii); and (iv) identifying as a TFP a nucleic acid fragment which induces the secretion of said target protein from the cell identified in (iii).
Method for evaluating the compactness of a layer of railway line ballast in the vicinity of a sleeper, characterized in that it comprises at least a step of taking at least two measurements (11, 11a, 11b) of the tip resistance ( Qd) of the ballast in the vicinity of the one same sleeper, and a step of calculating the mean value ( Qd moy) of these tip resistance ( Qd) measurements (11, 11a, 11b), in that each tip resistance ( Qd) measurement is performed using a penetrometer (1, 1a) which is driven into the ground by successive driving operations during each of which a value is obtained for the instantaneous tip resistance ( Qd i) of the ballast corresponding to each driving operation, and in that the said tip resistance ( Qd) is determined by averaging all the instantaneous tip resistance ( Qd i) values obtained previously.
Two-phase working fluid heat transfer device, comprising: - an evaporator including a liquid zone and a steam zone, - a condenser having at least one inlet and at least one outlet, - a first communication circuit, for working fluid essentially in the vapour phase, connecting the vapour zone of the evaporator to at least one inlet of the condenser, - a second communication circuit, for working fluid essentially in the liquid phase, connecting at least one outlet of the condenser to the liquid zone of the evaporator, - a reservoir containing working fluid with a liquid phase and a vapour phase, - an energy supply element, with selective control, adapted to increase the pressure of the working fluid contained in the reservoir, the reservoir being connected only to the evaporator, in its liquid zone, by means of a third communication circuit for working fluid essentially in liquid phase, a portion of the third communication circuit being thermally coupled to a first cold source. - an evaporator including a liquid zone and a steam zone, - a condenser having at least one inlet and at least one outlet, - a first communication circuit, for working fluid essentially in the vapour phase, connecting the vapour zone of the evaporator to at least one inlet of the condenser, - a second communication circuit, for working fluid essentially in the liquid phase, connecting at least one outlet of the condenser to the liquid zone of the evaporator, - a reservoir containing working fluid with a liquid phase and a vapour phase, - an energy supply element, with selective control, adapted to increase the pressure of the working fluid contained in the reservoir,
Measuring device for measuring electrical quantities, in particular electrical voltages and/or currents, comprising: a housing body comprising a single casing, at least one measuring device arranged or formed in the housing space for measuring an electrical quantity measurable by means of the measuring device, wherein the housing body has at least one receiving area for the detachable reception of an electrical energy storage device, wherein the receiving area is formed as a shaft-like or -shaped recess in the housing body, wherein the receiving area in which an electrical energy storage device to be absorbed in the receiving area is retractable, or as a plug-in area in which an electrical energy storage device to be absorbed in the receiving area is designed to be plugged in, characterised by: a detachable to the housing body connectable or connected to the housing body in the state connected to the housing body at least partially surrounding protective coating to protect the housing body from external influences, wherein the protective envelope is formed with at least one recess through which an electrical energy storage device received in the housing body side receiving area is removable from the receiving area, or an electrical energy storage device to be included in the housing body side receiving area is inserted into the receiving area. a housing body comprising a single casing, at least one measuring device arranged or formed in the housing space for measuring an electrical quantity measurable by means of the measuring device, wherein the housing body has at least one receiving area for the detachable reception of an electrical energy storage device, wherein the receiving area is formed as a shaft-like or -shaped recess in the housing body, wherein the receiving area in which an electrical energy storage device to be absorbed in the receiving area is retractable, or as a plug-in area in which an electrical energy storage device to be absorbed in the receiving area is designed to be plugged in, characterised by: a detachable to the housing body connectable or connected to the housing body in the state connected to the housing body at least partially surrounding protective coating to protect the housing body from external influences, wherein the protective envelope is formed with at least one recess through which an electrical energy storage device received in the housing body side receiving area is removable from the receiving area, or an electrical energy storage device to be included in the housing body side receiving area is inserted into the receiving area. a detachable to the housing body connectable or connected to the housing body in the state connected to the housing body at least partially surrounding protective coating to protect the housing body from external influences, wherein the protective envelope is formed with at least one recess through which an electrical energy storage device received in the housing body side receiving area is removable from the receiving area, or an electrical energy storage device to be included in the housing body side receiving area is inserted into the receiving area.
A centrifugal fan comprising: a hub to which a rotation shaft of a motor is coupled; a shroud disposed facing the hub; a plurality of first blades separated from each other and disposed along the circumferential direction of the hub; and a plurality of second blades disposed to be separated from each other along the circumferential direction of the hub while disposed to be separated from the plurality of first blades in a radial direction of the hub, wherein a guide member is configured to induce inflow in a middle part of the fan, wherein the guide member is disposed between the shroud and the hub to change a flow direction of air introduced in an axial direction of the hub to a circumferential direction of the hub, wherein the guide member is connected to the hub by the plurality of first blades and the guide member is connected to the shroud by the plurality of second blades, and wherein the guide member includes: an inlet-side planar region into which air is introduced; an outlet-side planar region from which the air is discharged; and a curved surface region which connects the inlet-side planar region to the outlet-side planar region. a hub to which a rotation shaft of a motor is coupled; a shroud disposed facing the hub; a plurality of first blades separated from each other and disposed along the circumferential direction of the hub; and a plurality of second blades disposed to be separated from each other along the circumferential direction of the hub while disposed to be separated from the plurality of first blades in a radial direction of the hub, wherein a guide member is configured to induce inflow in a middle part of the fan, wherein the guide member is disposed between the shroud and the hub to change a flow direction of air introduced in an axial direction of the hub to a circumferential direction of the hub, wherein the guide member is connected to the hub by the plurality of first blades and the guide member is connected to the shroud by the plurality of second blades, and wherein the guide member includes: an inlet-side planar region into which air is introduced; an outlet-side planar region from which the air is discharged; and a curved surface region which connects the inlet-side planar region to the outlet-side planar region. an inlet-side planar region into which air is introduced; an outlet-side planar region from which the air is discharged; and a curved surface region which connects the inlet-side planar region to the outlet-side planar region.
A secondary battery, comprising: a case having an accommodation space and a first opening in communication with the accommodation space; an electrode component disposed in the accommodation space; a cover plate being installed at the first opening, wherein at least a part of the cover plate extends into the first opening such that a connection slot is formed by the part of the cover plate extending into the first opening and an inner wall of the case, the connection slot is formed at a joint between the cover plate and the case, and the connection slot extends in a direction away from the accommodation space and throughout a top surface of the cover plate; and a first connection member being disposed in the connection slot, wherein the cover plate and the case are hermetically connected via the first connection member. a case having an accommodation space and a first opening in communication with the accommodation space; an electrode component disposed in the accommodation space; a cover plate being installed at the first opening, wherein at least a part of the cover plate extends into the first opening such that a connection slot is formed by the part of the cover plate extending into the first opening and an inner wall of the case, the connection slot is formed at a joint between the cover plate and the case, and the connection slot extends in a direction away from the accommodation space and throughout a top surface of the cover plate; and a first connection member being disposed in the connection slot, wherein the cover plate and the case are hermetically connected via the first connection member.
A thermoplastic resin composition comprising: a first graft copolymer including a first acrylic rubber polymer; a second graft copolymer including a second acrylic rubber polymer; a first styrene-based copolymer including an alkyl-substituted styrene-based monomer unit and an acrylonitrile-based monomer unit, a second styrene-based copolymer including an alkyl-unsubstituted styrene-based monomer unit and an acrylonitrile-based monomer unit, and an inorganic pigment, wherein the inorganic pigment is TiO 2; wherein the first acrylic rubber polymer has an average particle diameter larger than that of the second acrylic rubber polymer, the first graft copolymer is included in a larger amount than the second graft copolymer, the sum of the first graft copolymer and the second graft copolymer is 22 parts by weight or less with respect to 100 parts by weight of the sum of the first graft copolymer, the second graft copolymer, the first styrene-based copolymer and the second styrene-based copolymer, and wherein, with respect to 100 parts by weight of the sum of the first graft copolymer, the second graft copolymer, the first styrene-based copolymer, and the second styrene-based copolymer, 5 to 30 parts by weight of the first styrene-based copolymer and 50 to 80 parts by weight of the second styrene-based copolymer are included. a first graft copolymer including a first acrylic rubber polymer; a second graft copolymer including a second acrylic rubber polymer; a first styrene-based copolymer including an alkyl-substituted styrene-based monomer unit and an acrylonitrile-based monomer unit, a second styrene-based copolymer including an alkyl-unsubstituted styrene-based monomer unit and an acrylonitrile-based monomer unit, and an inorganic pigment, wherein the inorganic pigment is TiO 2; wherein the first acrylic rubber polymer has an average particle diameter larger than that of the second acrylic rubber polymer, the first graft copolymer is included in a larger amount than the second graft copolymer, the sum of the first graft copolymer and the second graft copolymer is 22 parts by weight or less with respect to 100 parts by weight of the sum of the first graft copolymer, the second graft copolymer, the first styrene-based copolymer and the second styrene-based copolymer, and wherein, with respect to 100 parts by weight of the sum of the first graft copolymer, the second graft copolymer, the first styrene-based copolymer, and the second styrene-based copolymer, 5 to 30 parts by weight of the first styrene-based copolymer and 50 to 80 parts by weight of the second styrene-based copolymer are included.
A reagent composition for nucleic acid chromatography or immunochromatography comprising: a water-soluble polymer having a weight average molecular weight of 8,000 or more, a salt of a divalent or trivalent metal, a nonionic surfactant, and an aprotic water-soluble organic compound which is selected from sulfoxides, N,N'-dialkylamides, ketones, nitriles, and cyclic ethers. a water-soluble polymer having a weight average molecular weight of 8,000 or more, a salt of a divalent or trivalent metal, a nonionic surfactant, and an aprotic water-soluble organic compound which is selected from sulfoxides, N,N'-dialkylamides, ketones, nitriles, and cyclic ethers.
A cold-rolled steel sheet having excellent high-temperature properties and room-temperature workability, comprising, by weight: carbon: 0.0005 to 0.003%, manganese (Mn): 0.20 to 0.50%, aluminum (Al): 0.01 to 0.10%, phosphorus: 0.003 to 0.020%, nitrogen: 0.0005 to 0.004%, sulfur: 0.015% or less, niobium (Nb): 0.005 to 0.040%, chromium (Cr): 0.10 to 0.50%, tungsten: 0.02 to 0.07%, and a balance of iron (Fe) and other inevitable impurities, wherein C, Nb, and W satisfy the following relationship 1, a microstructure comprises 95 area% or more of polygonal ferrite and the rest being 5 area% or less of acicular ferrite, and the cold-rolled steel sheet comprises (Nb,W)C-based precipitates having an average size of 0.005 to 0.10 μm, 0.00025 ≤ 2 × Nb/93 × W/184 / C/12 ≤ 0.0015 where, C, Nb, and W are in weight %. 0.003 to 0.020%, nitrogen: 0.0005 to 0.004%, sulfur: 0.015% or less, niobium (Nb): 0.005 to 0.040%, chromium (Cr): 0.10 to 0.50%, tungsten: 0.02 to 0.07%, and a balance of iron (Fe) and other inevitable impurities, wherein C, Nb, and W satisfy the following relationship 1, a microstructure comprises 95 area% or more of polygonal ferrite and the rest being 5 area% or less of acicular ferrite, and the cold-rolled steel sheet comprises (Nb,W)C-based precipitates having an average size of 0.005 to 0.10 μm, 0.00025 ≤ 2 × Nb/93 × W/184 / C/12 ≤ 0.0015 where, C, Nb, and W are in weight %.
A method comprising: at a first device (452, 862, 5302), receiving a probe signal sent from a second device (454, 864, 5304) through a multipath channel (472, 5306), the probe signal received at the first device having a waveform that is different from the waveform sent by the second device due to influence of the multipath channel; estimating a channel impulse response based on the probe signal received at the first device; determining a time-reversed signal based on a time-reversed version of the estimated channel impulse response; and determining a position of the second device (454, 864, 5304) based on convolving the time-reversed signal and stored channel impulse responses, wherein the stored channel impulse responses are stored in a storage device and are each associated with a known position. at a first device (452, 862, 5302), receiving a probe signal sent from a second device (454, 864, 5304) through a multipath channel (472, 5306), the probe signal received at the first device having a waveform that is different from the waveform sent by the second device due to influence of the multipath channel; estimating a channel impulse response based on the probe signal received at the first device; determining a time-reversed signal based on a time-reversed version of the estimated channel impulse response; and determining a position of the second device (454, 864, 5304) based on convolving the time-reversed signal and stored channel impulse responses, wherein the stored channel impulse responses are stored in a storage device and are each associated with a known position.
Method for correcting the state of a watch or respectively a movement, said watch or respectively said movement including at least a first oscillator arranged to generate oscillation at a nominal frequency, characterised in that there is used a state correction device including a second oscillator, this oscillator being a state correction oscillator, arranged to generate oscillation at a correction frequency to impose oscillation and/or a motion on said entire watch, or respectively on said entire movement, during a state correction phase after an initial moment of actuation of said state correction device, the duration of said state correction phase being adjusted to exactly correct a state error that is measured or evaluated on said watch or respectively said movement at said initial moment of actuation, in that the initial variation of rate value of said first oscillator is measured, and in that there is incorporated in said state correction device a winder for mechanical or automatic watches, arranged to move at least one support on which is fixed said at least one watch or respectively said at least one movement.
Wheel hub drive for a motor vehicle, having a motor, in particular an electric motor, which is connected to an input element of a transmission so as to exercise a driving effect, wherein the transmission has an output element, which is connected to a shaft section, by means of which a wheel of the motor vehicle can be driven, wherein a sensor is provided for monitoring the rotational speed of the drive, comprising a sensor element arranged in a manner fixed against rotation and a rotatable sensor element, wherein the rotatable sensor element is connected to the output element for conjoint rotation, wherein the rotatable sensor element is arranged on the circumference of the output element, characterized in that a wheel bearing section is provided to support the shaft section connected to the output element, wherein the outer circumference of the output element is larger than the outer circumference of the shaft section rotatably supported by the wheel bearing section and, in particular, is larger than the respective outer circumferences of further components connected to the shaft section for conjoint rotation, and wherein the transmission is arranged in a housing or housing section which can be closed by means of a cover, wherein the sensor element fixed against rotation is arranged on the cover and, in particular, extends at least partially into the interior of the housing through an opening in the cover.
Arrangement (1, 32) for a test device (3, 31) for small-volume bulk materials (9 to 12, 42 to 44, 61 to 64) and a dedusting system (2, 30), wherein the dedusting system (2, 30) is mounted on the test apparatus, and wherein the dedusting system (2, 30) comprises: - a bottom (4, 33) which in a first section (8, 45) has a plurality of openings (13 to 16, 46 to 48) having a screening function, wherein dust can be extracted via these openings (13 to 16, 46 to 48) from the dedusting system (2, 30) and wherein the bulk materials (9 to 12, 42 to 44, 61 to 64) remain on the floor (4, 33), wherein the floor (4, 33) has a second section (24, 56) in which a discharge opening (25, 57) is provided, via which the bulk materials (9 to 12, 42 to 44, 61 to 64) can be removed from the dedusting system (2, 30) and fed to the test apparatus (3, 31), - a top (5, 34) movably arranged above the bottom (4, 33), wherein the top (5, 34) has a chamber (7, 40) which is open downwardly, so that a part of the bottom (4, 33) forms the bottom of the chamber (7, 40), and wherein the chamber (7, 40) has a charging opening (19, 60) via which the bulk materials (9 to 12, 42 to 44, 61 to 64) can be introduced into the chamber (7, 40), wherein in a first position, the chamber (7, 40) of the arrangement (5, 34) is arranged above the plurality of openings (13 to 16, 46 to 48) having a screening function, and wherein in a second position, the chamber (7, 40) of the arrangement (5, 34) is arranged above the discharge opening (25, 57). - a bottom (4, 33) which in a first section (8, 45) has a plurality of openings (13 to 16, 46 to 48) having a screening function, wherein dust can be extracted via these openings (13 to 16, 46 to 48) from the dedusting system (2, 30) and wherein the bulk materials (9 to 12, 42 to 44, 61 to 64) remain on the floor (4, 33), wherein the floor (4, 33) has a second section (24, 56) in which a discharge opening (25, 57) is provided, via which the bulk materials (9 to 12, 42 to 44, 61 to 64) can be removed from the dedusting system (2, 30) and fed to the test apparatus (3, 31), - a top (5, 34) movably arranged above the bottom (4, 33), wherein the top (5, 34) has a chamber (7, 40) which is open downwardly, so that a part of the bottom (4, 33) forms the bottom of the chamber (7, 40), and wherein the chamber (7, 40) has a charging opening (19, 60) via which the bulk materials (9 to 12, 42 to 44, 61 to 64) can be introduced into the chamber (7, 40), wherein in a first position, the chamber (7, 40) of the arrangement (5, 34) is arranged above the plurality of openings (13 to 16, 46 to 48) having a screening function, and wherein in a second position, the chamber (7, 40) of the arrangement (5, 34) is arranged above the discharge opening (25, 57). wherein in a first position, the chamber (7, 40) of the arrangement (5, 34) is arranged above the plurality of openings (13 to 16, 46 to 48) having a screening function, and wherein in a second position, the chamber (7, 40) of the arrangement (5, 34) is arranged above the discharge opening (25, 57).