A cell-free protein expression system comprising: - a cell-free expression bioreactor having: - a reaction mixture containing a cell extract having all cell-free reaction components necessary for in vitro macromolecule synthesis; - wherein said cell extract is derived from Geobacillus bacteria genetically modified to include any combination of the following: - knock-out expression of OmpT-homologue; - knock-out expression of RNaseI; - knock-out expression of DNA-methylation dependent DNase; - reduce expression of culture density-dependent sporulation operon; - overexpress sigma factor RpoD; and - overexpress RNA polymerase (RNAP); - at least one nucleic acid synthesis template; - a cellular adenosine triphosphate energy regeneration system comprising: - a quantity of purified Adenosyl Kinase (AdK) enzyme derived from Geobacillus stearothermophilus (Gst Adk); - a quantity of purified Polyphosphate Kinase enzyme derived from Thermus aquaticus (TaqPPK); - a quantity of inorganic polyphosphate (PPi); and - a quantity of adenosine monophosphate; - wherein said AdK and PPK enzymes work synergistically to regenerate cellular ATP energy from PPi and AMP; and, wherein the ratio of Gst AdK to TaqPPK includes higher amounts of Gst AdK as compared to TaqPPK. - a cell-free expression bioreactor having: - a reaction mixture containing a cell extract having all cell-free reaction components necessary for in vitro macromolecule synthesis; - wherein said cell extract is derived from Geobacillus bacteria genetically modified to include any combination of the following: - knock-out expression of OmpT-homologue; - knock-out expression of RNaseI; - knock-out expression of DNA-methylation dependent DNase; - reduce expression of culture density-dependent sporulation operon; - overexpress sigma factor RpoD; and - overexpress RNA polymerase (RNAP); - at least one nucleic acid synthesis template; - a cellular adenosine triphosphate energy regeneration system comprising: - a quantity of purified Adenosyl Kinase (AdK) enzyme derived from Geobacillus stearothermophilus (Gst Adk); - a quantity of purified Polyphosphate Kinase enzyme derived from Thermus aquaticus (TaqPPK); - a quantity of inorganic polyphosphate (PPi); and - a quantity of adenosine monophosphate; - wherein said AdK and PPK enzymes work synergistically to regenerate cellular ATP energy from PPi and AMP; and, wherein the ratio of Gst AdK to TaqPPK includes higher amounts of Gst AdK as compared to TaqPPK. - a reaction mixture containing a cell extract having all cell-free reaction components necessary for in vitro macromolecule synthesis; - wherein said cell extract is derived from Geobacillus bacteria genetically modified to include any combination of the following: - knock-out expression of OmpT-homologue; - knock-out expression of RNaseI; - knock-out expression of DNA-methylation dependent DNase; - reduce expression of culture density-dependent sporulation operon; - overexpress sigma factor RpoD; and - overexpress RNA polymerase (RNAP); - knock-out expression of OmpT-homologue; - knock-out expression of RNaseI; - knock-out expression of DNA-methylation dependent DNase; - reduce expression of culture density-dependent sporulation operon; - overexpress sigma factor RpoD; and - overexpress RNA polymerase (RNAP); - at least one nucleic acid synthesis template; - a cellular adenosine triphosphate energy regeneration system comprising: - a quantity of purified Adenosyl Kinase (AdK) enzyme derived from Geobacillus stearothermophilus (Gst Adk); - a quantity of purified Polyphosphate Kinase enzyme derived from Thermus aquaticus (TaqPPK); - a quantity of inorganic polyphosphate (PPi); and - a quantity of adenosine monophosphate; - a quantity of purified Adenosyl Kinase (AdK) enzyme derived from Geobacillus stearothermophilus (Gst Adk); - a quantity of purified Polyphosphate Kinase enzyme derived from Thermus aquaticus (TaqPPK); - a quantity of inorganic polyphosphate (PPi); and - a quantity of adenosine monophosphate; - wherein said AdK and PPK enzymes work synergistically to regenerate cellular ATP energy from PPi and AMP; and, wherein the ratio of Gst AdK to TaqPPK includes higher amounts of Gst AdK as compared to TaqPPK.