An apparatus for making a sterile connection, comprising: a container (106, 306); a sealing member (158, 400) sealing a first end of the container; a barrier (154, 406) sealing a chamber in fluid communication with the sealing member; and a valve sleeve assembly, comprising: a flexible sleeve (152, 382) having a barrier portion (153, 384) at an end of the flexible sleeve, an interior of the flexible sleeve being sterile; a hollow first penetrator (148, 388) adapted to displace relative to the flexible sleeve; and a hollow second penetrator (159, 390) at least partially disposed within the first penetrator and adapted to displace relative to the first penetrator and the flexible sleeve; wherein in an initial state, the flexible sleeve encloses the first penetrator and a penetrating end of the second penetrator; wherein one of the container and the valve sleeve assembly is adapted to displace relative to the remaining one of the container and the valve sleeve assembly; wherein the flexible sleeve is adapted to collapse, thereby displacing the outer penetrator relative to the flexible sleeve to pierce the barrier portion and the barrier; and wherein upon further relative displacement between the container and the valve sleeve assembly, subsequent to the piercing of the barrier portion and the barrier, the second penetrator is adapted to displace relative to the first penetrator to pierce the sealing member to create a sterile connection with the interior of the container. a container (106, 306); a sealing member (158, 400) sealing a first end of the container; a barrier (154, 406) sealing a chamber in fluid communication with the sealing member; and a valve sleeve assembly, comprising: a flexible sleeve (152, 382) having a barrier portion (153, 384) at an end of the flexible sleeve, an interior of the flexible sleeve being sterile; a hollow first penetrator (148, 388) adapted to displace relative to the flexible sleeve; and a hollow second penetrator (159, 390) at least partially disposed within the first penetrator and adapted to displace relative to the first penetrator and the flexible sleeve; a flexible sleeve (152, 382) having a barrier portion (153, 384) at an end of the flexible sleeve, an interior of the flexible sleeve being sterile; a hollow first penetrator (148, 388) adapted to displace relative to the flexible sleeve; and a hollow second penetrator (159, 390) at least partially disposed within the first penetrator and adapted to displace relative to the first penetrator and the flexible sleeve; wherein in an initial state, the flexible sleeve encloses the first penetrator and a penetrating end of the second penetrator; wherein one of the container and the valve sleeve assembly is adapted to displace relative to the remaining one of the container and the valve sleeve assembly; wherein the flexible sleeve is adapted to collapse, thereby displacing the outer penetrator relative to the flexible sleeve to pierce the barrier portion and the barrier; and wherein upon further relative displacement between the container and the valve sleeve assembly, subsequent to the piercing of the barrier portion and the barrier, the second penetrator is adapted to displace relative to the first penetrator to pierce the sealing member to create a sterile connection with the interior of the container.