A method of obtaining plant derived virus-like particles (VLPs); or plant derived proteins or protein suprastructures, from a plant or plant matter comprising apoplast-localized VLPs, apoplast-localized proteins or protein suprastructures, the plant matter consisting of plant leaves, the method comprising: a. producing a digested fraction comprising an apoplast fraction, the apoplast fraction comprising disrupted protoplasts, by extraction from the plant or plant matter, wherein the extraction consists of an enzymatic extraction with a cell wall degrading enzyme mixture consisting of: one or more than one cellulase and one or more than one pectinase, about 20 mM to about 750 mM of a salt; 0 mM to about 200 mM of a chelator; 0.005% to 0.4% of a reducing agent selected from sodium bisulfite and ascorbic acid; and a buffer or buffer system which maintains the pH in the range of about 5 to about 6.6, at room temperature, or one or more than one cellulase and one or more than one pectinase, about 20 mM to about 750 mM of a salt; 0 mM to about 200 mM of a chelator; 0.005% to 0.4% of a reducing agent selected from sodium bisulfite and ascorbic acid; and one or more components selected from the group consisting of an osmoticum, an enzyme inhibitor, and an inhibitor of foliar senescence, and a buffer or buffer system which maintains the pH in the range of about 5 to about 6.6, at room temperature, and b. recovering the apoplast fraction from the digested fraction, the apoplast fraction comprising the plant-derived VLPs, or the plant-derived proteins or protein suprastructures, wherein the protein suprastructures have a molecular weight from about 75 to about 1500 kDa, wherein the step of recovering (step b.) comprises removing cellular debris and insoluble materials to obtain the plant derived virus-like particles (VLPs), or plant derived proteins or protein suprastructures. a. producing a digested fraction comprising an apoplast fraction, the apoplast fraction comprising disrupted protoplasts, by extraction from the plant or plant matter, wherein the extraction consists of an enzymatic extraction with a cell wall degrading enzyme mixture consisting of: one or more than one cellulase and one or more than one pectinase, about 20 mM to about 750 mM of a salt; 0 mM to about 200 mM of a chelator; 0.005% to 0.4% of a reducing agent selected from sodium bisulfite and ascorbic acid; and a buffer or buffer system which maintains the pH in the range of about 5 to about 6.6, at room temperature, or one or more than one cellulase and one or more than one pectinase, about 20 mM to about 750 mM of a salt; 0 mM to about 200 mM of a chelator; 0.005% to 0.4% of a reducing agent selected from sodium bisulfite and ascorbic acid; and one or more components selected from the group consisting of an osmoticum, an enzyme inhibitor, and an inhibitor of foliar senescence, and a buffer or buffer system which maintains the pH in the range of about 5 to about 6.6, at room temperature, and one or more than one cellulase and one or more than one pectinase, about 20 mM to about 750 mM of a salt; 0 mM to about 200 mM of a chelator; 0.005% to 0.4% of a reducing agent selected from sodium bisulfite and ascorbic acid; and a buffer or buffer system which maintains the pH in the range of about 5 to about 6.6, at room temperature, or about 20 mM to about 750 mM of a salt; 0 mM to about 200 mM of a chelator; 0.005% to 0.4% of a reducing agent selected from sodium bisulfite and ascorbic acid; and a buffer or buffer system which maintains the pH in the range of about 5 to about 6.6, at room temperature, or one or more than one cellulase and one or more than one pectinase, about 20 mM to about 750 mM of a salt; 0 mM to about 200 mM of a chelator; 0.005% to 0.4% of a reducing agent selected from sodium bisulfite and ascorbic acid; and one or more components selected from the group consisting of an osmoticum, an enzyme inhibitor, and an inhibitor of foliar senescence, and a buffer or buffer system which maintains the pH in the range of about 5 to about 6.6, at room temperature, and about 20 mM to about 750 mM of a salt; 0 mM to about 200 mM of a chelator; 0.005% to 0.4% of a reducing agent selected from sodium bisulfite and ascorbic acid; and one or more components selected from the group consisting of an osmoticum, an enzyme inhibitor, and an inhibitor of foliar senescence, and a buffer or buffer system which maintains the pH in the range of about 5 to about 6.6, at room temperature, and b. recovering the apoplast fraction from the digested fraction, the apoplast fraction comprising the plant-derived VLPs, or the plant-derived proteins or protein suprastructures, wherein the protein suprastructures have a molecular weight from about 75 to about 1500 kDa, wherein the step of recovering (step b.) comprises removing cellular debris and insoluble materials to obtain the plant derived virus-like particles (VLPs), or plant derived proteins or protein suprastructures.