mhurhangee/ep-ind-claims huggingface dataset:
Method of producing a product of interest with microbial cells, comprising the steps: a) culturing the microbial cells which are capable of producing said product of interest in a culture medium; b) disrupting the microbial cells by incubating them at a temperature of 45 to 55°C for 2 to 10 hours, thereby releasing the DNA from the cells; c) incubating the released DNA at a temperature of at least 50°C and a pH of less than 4.5, thereby degrading the released DNA; and d) isolating the product of interest, wherein no complete coding sequence of a gene is detectable in the product of interest isolated in step d) and wherein the microbial cells are selected from the group consisting of Eremothecium gossypii, Bacillus subtilis and Corynebacterium glutamicum cells. a) culturing the microbial cells which are capable of producing said product of interest in a culture medium; b) disrupting the microbial cells by incubating them at a temperature of 45 to 55°C for 2 to 10 hours, thereby releasing the DNA from the cells; c) incubating the released DNA at a temperature of at least 50°C and a pH of less than 4.5, thereby degrading the released DNA; and d) isolating the product of interest,
A method for preparing a nucleic acid library, which comprises: providing a plurality of nucleic acid molecules from a sample; and manipulating the plurality of nucleic acid molecules in a reagent comprising a desferrioxamine B (DFO-B) mesylate salt and the reagent does not contain EDTA. providing a plurality of nucleic acid molecules from a sample; and manipulating the plurality of nucleic acid molecules in a reagent comprising a desferrioxamine B (DFO-B) mesylate salt and the reagent does not contain EDTA.
A robot cleaner, comprising: a housing (112, 114) comprising a suction inlet configured to suction foreign substances, a suction motor configured to generate a suction force, and a discharge outlet configured to discharge the suctioned air to an outside thereof; a driver configured to move the robot cleaner; an agitator disposed under the housing (112, 114) and configured to move the foreign substances located on the floor to the suction inlet by performing rotation; a constraint prevention filter portion configured to perform a function for blocking entry of an obstacle provided at a position in a moving direction of the robot cleaner into the agitator, the constraint prevention filter portion comprising a constraint prevention filter, a rotary shaft to couple to the constraint prevention filter, and a filter driver to control a position of the rotary shaft; a sensor disposed on the housing (112, 114) and configured to detect an obstacle positioned in the first direction; and a controller configured to control the operation of each of the driver and the filter driver based on types of obstacles detected by the sensor, characterized in that the constraint prevention filter has a shred shape including a plurality of thin and long strings and prevents the obstacle of a predetermined size or more from entering the inner side of the main housing, and in that the constraint prevention filter is positioned to face the moving direction with respect to the housing (112, 114). a housing (112, 114) comprising a suction inlet configured to suction foreign substances, a suction motor configured to generate a suction force, and a discharge outlet configured to discharge the suctioned air to an outside thereof; a driver configured to move the robot cleaner; an agitator disposed under the housing (112, 114) and configured to move the foreign substances located on the floor to the suction inlet by performing rotation; a constraint prevention filter portion configured to perform a function for blocking entry of an obstacle provided at a position in a moving direction of the robot cleaner into the agitator, the constraint prevention filter portion comprising a constraint prevention filter, a rotary shaft to couple to the constraint prevention filter, and a filter driver to control a position of the rotary shaft; a sensor disposed on the housing (112, 114) and configured to detect an obstacle positioned in the first direction; and a controller configured to control the operation of each of the driver and the filter driver based on types of obstacles detected by the sensor, characterized in that the constraint prevention filter has a shred shape including a plurality of thin and long strings and prevents the obstacle of a predetermined size or more from entering the inner side of the main housing, and in that the constraint prevention filter is positioned to face the moving direction with respect to the housing (112, 114).
One-peace woven airbag having warp threads and weft threads, which are woven together in at least three woven fabric layers, a lower fabric layer, an upper fabric layer and a middle fabric layer arranged therebetween, characterized in that the warp and weft threads of the middle fabric layer emerge from said middle fabric layer in a first partial region of the OPW airbag and float completely between the lower fabric layer and the upper fabric layer and are incorporated into the lower fabric layer or into the upper fabric layer in a second partial region of the OPW airbag.
A method for manufacturing a marked toilet paper roll by winding a sheet of wide toilet paper a predetermined length about a winding shaft to form a log roll and by cutting the log roll at a predetermined width into a plurality of toilet paper rolls, the method comprising the steps of: applying a liquid glue on the end portion of a winding end of the log roll thereby adhering the end portion to an outer surface of the log roll; and, after the adhering, marking stamps at intervals, each interval being of the predetermined width, on an end portion of a winding end of the log roll before the log roll is cut into the toilet paper rolls, wherein, in the marking step, a roll that includes stamp portions provided at intervals of the predetermined width is used and the stamps are applied by having the stamp portions come into surface contact with the end portion of the winding end while the roll is rotated so as to synchronize with the rotation of the log roll. applying a liquid glue on the end portion of a winding end of the log roll thereby adhering the end portion to an outer surface of the log roll; and, after the adhering, marking stamps at intervals, each interval being of the predetermined width, on an end portion of a winding end of the log roll before the log roll is cut into the toilet paper rolls, wherein, in the marking step, a roll that includes stamp portions provided at intervals of the predetermined width is used and the stamps are applied by having the stamp portions come into surface contact with the end portion of the winding end while the roll is rotated so as to synchronize with the rotation of the log roll.
A press machine that converts a rotation of a motor into a reciprocating linear motion of a slide and performs press working on a workpiece material, the press machine comprising: a press control unit that controls ascending/descending motion of the slide; and an abnormality diagnostic unit configured to perform abnormality diagnosis of the press machine based on data from a sensor (22, 23) provided in the press machine and to output a result of the abnormality diagnosis, characterized in that the press control unit is configured to output a diagnosis start request to the abnormality diagnostic unit when starting ascending or descending the slide, the abnormality diagnostic unit being configured to start acquiring the data from the sensor (22, 23) when receiving the diagnostic start request, and perform the abnormality diagnosis based on the data acquired from the sensor (22, 23) while the slide moves at a predetermined speed between a bottom dead point and a top dead point in a press-no-load state. a press control unit that controls ascending/descending motion of the slide; and an abnormality diagnostic unit configured to perform abnormality diagnosis of the press machine based on data from a sensor (22, 23) provided in the press machine and to output a result of the abnormality diagnosis, characterized in that the press control unit is configured to output a diagnosis start request to the abnormality diagnostic unit when starting ascending or descending the slide, the abnormality diagnostic unit being configured to start acquiring the data from the sensor (22, 23) when receiving the diagnostic start request, and perform the abnormality diagnosis based on the data acquired from the sensor (22, 23) while the slide moves at a predetermined speed between a bottom dead point and a top dead point in a press-no-load state.
A receiver comprising: a storage unit adapted to store a signaling template, the signaling template being a template for generating a signaling instance, the signaling instance including signaling data required for reception and reproduction of a content; a communication unit adapted to receive difference information as data to be applied to the signaling template and used for generating a signaling instance; and a data processing unit adapted to generate the signaling instance by applying the difference information to the signaling template, wherein the difference information includes: an identifier of a signaling template to which difference information is applied; and an identifier of a signaling instance generated by the process of applying the difference information to the signaling template, the data processing unit is adapted to set a signaling instance identifier recorded in difference information to the signaling instance generated as a result of executing the process of applying the difference information by selecting and applying a signaling template including the same identifier as the identifier of the signaling template recorded in the difference information. a storage unit adapted to store a signaling template, the signaling template being a template for generating a signaling instance, the signaling instance including signaling data required for reception and reproduction of a content; a communication unit adapted to receive difference information as data to be applied to the signaling template and used for generating a signaling instance; and a data processing unit adapted to generate the signaling instance by applying the difference information to the signaling template, wherein the difference information includes: an identifier of a signaling template to which difference information is applied; and an identifier of a signaling instance generated by the process of applying the difference information to the signaling template, an identifier of a signaling template to which difference information is applied; and an identifier of a signaling instance generated by the process of applying the difference information to the signaling template, the data processing unit is adapted to set a signaling instance identifier recorded in difference information to the signaling instance generated as a result of executing the process of applying the difference information by selecting and applying a signaling template including the same identifier as the identifier of the signaling template recorded in the difference information.
A bicycle signal output device comprising: a magnet structured to be mounted to one of a stationary part and a moving part of a bicycle; and a rotation sensor comprising: a coil structured to be mounted to the other one of the stationary part and the moving part of the bicycle; a signal generating unit that operates using electrical power generated by the coil and generates rotation signals in response to relative motion between the magnet and the coil (12; and a wireless transmitting unit that wirelessly transmits the generated rotation signals, characterized in that the rotation sensor further comprises a rectifier configured to rectify and condition the electrical power generated by the coil, and to output the rectified and conditioned electrical power to the signal generating unit such that the signal generating unit operates using the electrical power from the rectifier and the signal generating unit comprises a microcomputer that operates using the electrical power from the rectifier and generates the rotation signals using appropriate software a magnet structured to be mounted to one of a stationary part and a moving part of a bicycle; and a rotation sensor comprising: a coil structured to be mounted to the other one of the stationary part and the moving part of the bicycle; a signal generating unit that operates using electrical power generated by the coil and generates rotation signals in response to relative motion between the magnet and the coil (12; and a wireless transmitting unit that wirelessly transmits the generated rotation signals, characterized in that the rotation sensor further comprises a rectifier configured to rectify and condition the electrical power generated by the coil, and to output the rectified and conditioned electrical power to the signal generating unit such that the signal generating unit operates using the electrical power from the rectifier and the signal generating unit comprises a microcomputer that operates using the electrical power from the rectifier and generates the rotation signals using appropriate software a coil structured to be mounted to the other one of the stationary part and the moving part of the bicycle; a signal generating unit that operates using electrical power generated by the coil and generates rotation signals in response to relative motion between the magnet and the coil (12; and a wireless transmitting unit that wirelessly transmits the generated rotation signals, characterized in that the rotation sensor further comprises a rectifier configured to rectify and condition the electrical power generated by the coil, and to output the rectified and conditioned electrical power to the signal generating unit such that the signal generating unit operates using the electrical power from the rectifier and the signal generating unit comprises a microcomputer that operates using the electrical power from the rectifier and generates the rotation signals using appropriate software
A miniature atomic clock with pulse mode operation, comprising: - a local oscillator having a microwave frequency; - a dual-frequency laser source receiving an output signal from the local oscillator and having an optical frequency; - means configured to pulse an output signal from the dual-frequency laser source, according to a Ramsey-type interrogation sequence comprising pulses with a period T1 separated by intervals with a period T2; - an alkaline vapour microcell receiving the output signal from the dual-frequency laser source and using coherent population trapping; - a photodiode receiving an output signal from the alkaline vapour microcell; - a feedback control loop for controlling microwave frequency, receiving an output signal from the photodiode and configured to servo-control the microwave frequency of the local oscillator to a microwave frequency setpoint depending on the alkaline vapour microcell; and - a feedback control loop for controlling optical frequency, configured to servo-control the optical frequency of the dual-frequency laser source to an optical frequency setpoint; characterised in that the feedback control loop for servo-controlling optical frequency comprises a pulse control block (SERVO_2') receiving the output signal from the photodiode and the interrogation sequence, and providing a correction signal (δ_c_2') to the dual-frequency laser source while being configured to act as follows: - during the period T1, extracting an error signal (δ_e_2') from the output signal received from the photodiode and generating the correction signal (δ_c_2') from the error signal; and - during the period T2, resetting the error signal (δ_e_2') to zero and generating the correction signal (δ_c_2') by extrapolation. - a local oscillator having a microwave frequency; - a dual-frequency laser source receiving an output signal from the local oscillator and having an optical frequency; - means configured to pulse an output signal from the dual-frequency laser source, according to a Ramsey-type interrogation sequence comprising pulses with a period T1 separated by intervals with a period T2; - an alkaline vapour microcell receiving the output signal from the dual-frequency laser source and using coherent population trapping; - a photodiode receiving an output signal from the alkaline vapour microcell; - a feedback control loop for controlling microwave frequency, receiving an output signal from the photodiode and configured to servo-control the microwave frequency of the local oscillator to a microwave frequency setpoint depending on the alkaline vapour microcell; and - a feedback control loop for controlling optical frequency, configured to servo-control the optical frequency of the dual-frequency laser source to an optical frequency setpoint; - during the period T1, extracting an error signal (δ_e_2') from the output signal received from the photodiode and generating the correction signal (δ_c_2') from the error signal; and - during the period T2, resetting the error signal (δ_e_2') to zero and generating the correction signal (δ_c_2') by extrapolation.
A mold assembly for an injection molding system, the mold assembly comprising: a top portion, the top portion including one or more cassette alignment pins extending therefrom; a precision cassette plate, the cassette plate including one or more openings in which parts are fabricated and secured, one or more pre-alignment through-holes which engage the one or more cassette alignment pins, and two anti-rotational features (806,808); a runner/gate insert element, the runner/gate insert element including a sprue channel and one or more runners and gates, the number of runners and gates equals the number of openings in the precision cassette plate; and a base portion, including an opening through which a nozzle of an injection molding system is positioned and makes contact with the sprue channel of the runner/gate inset element, wherein the top portion further comprises one or more cavity core pins extending therefrom for forming cavities within the parts, the number of cavity core pins equals the number of openings in the precision cassette plate. a top portion, the top portion including one or more cassette alignment pins extending therefrom; a precision cassette plate, the cassette plate including one or more openings in which parts are fabricated and secured, one or more pre-alignment through-holes which engage the one or more cassette alignment pins, and two anti-rotational features (806,808); a runner/gate insert element, the runner/gate insert element including a sprue channel and one or more runners and gates, the number of runners and gates equals the number of openings in the precision cassette plate; and a base portion, including an opening through which a nozzle of an injection molding system is positioned and makes contact with the sprue channel of the runner/gate inset element, wherein the top portion further comprises one or more cavity core pins extending therefrom for forming cavities within the parts, the number of cavity core pins equals the number of openings in the precision cassette plate.
A medical tube which is provided with a first site, a second site and a third site in this order from the proximal end side toward the distal end side and is made of resin, wherein at the second site, the outer diameter thereof is smaller than the outer diameter of the first site and the inner diameter is substantially equal to the inner diameter of the first site, and wherein at the third site, the outer diameter and the inner diameter thereof are respectively smaller than the outer diameter and the inner diameter of the second site, characterized in that the first site, the second site, and the third site are integrally formed of the same material.
A wavelength conversion element comprising: a substrate; a wavelength conversion layer, disposed on the substrate, wherein the wavelength conversion layer includes an inorganic adhesive and a wavelength conversion material, and the wavelength conversion material is mixed with the inorganic adhesive, wherein the wavelength conversion layer has a first surface and a second surface relative to the first surface; a first inorganic interstitial layer, disposed between the wavelength conversion layer and the substrate, wherein the first inorganic interstitial layer is formed on the first surface and is configured to fill pores of the wavelength conversion layer; and a second inorganic interstitial layer, wherein the wavelength conversion layer is disposed between the second inorganic interstitial layer and the first inorganic interstitial layer, and wherein a thickness of the second inorganic interstitial layer in a direction perpendicular to the substrate increases from a center toward an edge, or decreases from a center toward an edge, and wherein the second inorganic interstitial layer is formed on the second surface and is configured to fill pores of the wavelength conversion layer. a substrate; a wavelength conversion layer, disposed on the substrate, wherein the wavelength conversion layer includes an inorganic adhesive and a wavelength conversion material, and the wavelength conversion material is mixed with the inorganic adhesive, wherein the wavelength conversion layer has a first surface and a second surface relative to the first surface; a first inorganic interstitial layer, disposed between the wavelength conversion layer and the substrate, wherein the first inorganic interstitial layer is formed on the first surface and is configured to fill pores of the wavelength conversion layer; and a second inorganic interstitial layer, wherein the wavelength conversion layer is disposed between the second inorganic interstitial layer and the first inorganic interstitial layer, and wherein a thickness of the second inorganic interstitial layer in a direction perpendicular to the substrate increases from a center toward an edge, or decreases from a center toward an edge, and wherein the second inorganic interstitial layer is formed on the second surface and is configured to fill pores of the wavelength conversion layer.
A process for purifying pneumocandin B 0, having one or more polar impurities and one or more non-polar impurities, said process comprising the acts of: a) extracting the product from the fermentation broth by extracting the same with a suitable solvent and partially concentrating, wherein the suitable solvent is an alcohol selected from n-butanol, sec-butanol, tertiary-butanol and n-propanol; b) washing with immiscible solvent; c) charcoalizing; d) concentrating and filtering; e) crystallizing the filtrate to obtain a solid; f) loading the solid into a column with an adsorbent selected from alumina or silica gel; g) eluting impurities by passing one column volume of water through the column; h) eluting the product from the column with one or more solvent(s) to obtain eluted pneumocandin Bo; and i) concentrating and crystallizing the eluted pneumocandin B 0 to obtain the pneumocandin B 0. a) extracting the product from the fermentation broth by extracting the same with a suitable solvent and partially concentrating, wherein the suitable solvent is an alcohol selected from n-butanol, sec-butanol, tertiary-butanol and n-propanol; b) washing with immiscible solvent; c) charcoalizing; d) concentrating and filtering; e) crystallizing the filtrate to obtain a solid; f) loading the solid into a column with an adsorbent selected from alumina or silica gel; g) eluting impurities by passing one column volume of water through the column; h) eluting the product from the column with one or more solvent(s) to obtain eluted pneumocandin Bo; and i) concentrating and crystallizing the eluted pneumocandin B 0 to obtain the pneumocandin B 0.