A multilayer microfluidic probe head comprising a first layer, a second layer, and a first tubing port extending from an upper face of the first layer, wherein: the first layer has a first via enabling fluid communication between the first port and a lower face of the first layer; the second layer comprises: a first aperture on a face thereof; and a first microchannel enabling fluid communication between an upper face of the second layer, facing the lower face of the first layer, and the first aperture; and the head is further configured to enable fluid communication between the first via and the first microchannel, and wherein at least a portion of the first microchannel is a groove open on the upper face of the second layer, and closed by a portion of a lower face of a layer of the head (110, 130), the microfluidic probe head further comprises a second tubing port, a second via, a second aperture and a second microchannel, configured similarly as the first tubing port, the first via, the first aperture and the first microchannel, whereby each of the apertures (121, 122) is open on a same face, the latter being said face of the second layer, with the first aperture distinct from the second aperture, the head is further configured to: enable distinct fluid communications between the first via and the first aperture on the one hand, and between the second via and the second aperture on the other hand; and allow for aspirating at one of the apertures some fluid deposited via the other aperture on a surface close to the apertures, and said face of the second layer is either: a lower face of the second layer; or a face perpendicular to the upper face of the second layer, whereby at least a portion of each the first microchannel and the second microchannel is a groove (123, 124) extending up to one of the first aperture or the second aperture formed at an end of the groove at the level of an edge of the upper face of the second layer. the first layer has a first via enabling fluid communication between the first port and a lower face of the first layer; the second layer comprises: a first aperture on a face thereof; and a first microchannel enabling fluid communication between an upper face of the second layer, facing the lower face of the first layer, and the first aperture; and the head is further configured to enable fluid communication between the first via and the first microchannel, and wherein at least a portion of the first microchannel is a groove open on the upper face of the second layer, and closed by a portion of a lower face of a layer of the head (110, 130), the microfluidic probe head further comprises a second tubing port, a second via, a second aperture and a second microchannel, configured similarly as the first tubing port, the first via, the first aperture and the first microchannel, whereby each of the apertures (121, 122) is open on a same face, the latter being said face of the second layer, with the first aperture distinct from the second aperture, the head is further configured to: enable distinct fluid communications between the first via and the first aperture on the one hand, and between the second via and the second aperture on the other hand; and allow for aspirating at one of the apertures some fluid deposited via the other aperture on a surface close to the apertures, and enable distinct fluid communications between the first via and the first aperture on the one hand, and between the second via and the second aperture on the other hand; and allow for aspirating at one of the apertures some fluid deposited via the other aperture on a surface close to the apertures, and said face of the second layer is either: a lower face of the second layer; or a face perpendicular to the upper face of the second layer, whereby at least a portion of each the first microchannel and the second microchannel is a groove (123, 124) extending up to one of the first aperture or the second aperture formed at an end of the groove at the level of an edge of the upper face of the second layer. a lower face of the second layer; or a face perpendicular to the upper face of the second layer, whereby at least a portion of each the first microchannel and the second microchannel is a groove (123, 124) extending up to one of the first aperture or the second aperture formed at an end of the groove at the level of an edge of the upper face of the second layer.