A printing apparatus comprising: a processing means configured to perform processing relating to color calibration in the printing apparatus that prints an image on a printing medium using a fluorescent printing material, wherein the fluorescent printing material is a color material that develops a color by entering an activated state by absorbing light having an activation wavelength from a ground state and returning to the ground state by emitting light having a light-emitting wavelength; and an execution means configured to perform calibration processing of the printing apparatus based on a measured value that is obtained by measuring a patch chart for obtaining information on an amount of application of the fluorescent printing material on the printing medium, wherein the printing apparatus is configured to print a patch included in the patch chart with the fluorescent printing material and a subtractive color mixture printing material so that at least one dot of the subtractive color mixture printing material covers a dot of the fluorescent printing material on the printing medium, wherein the subtractive color mixture printing material contains a color material that absorbs light having a specific wavelength and does not emit the light among cast light, and wherein the printing apparatus further comprises a first measuring unit for measuring the patch chart, the first measuring unit including one or a plurality of light sources and a light-receiving element and configured to measure the patch included in the patch chart, wherein the first measuring unit is configured to cast light of the light source whose spectral distribution exists in a wavelength area including activation wavelengths of the fluorescent printing material to the patch, characterized in that the processing relating to color calibration comprises: selecting a subtractive color mixture printing material as the subtractive color mixture printing material, whose spectral reflectance in an area of the activation wavelength of the fluorescent printing material is high and whose reflectance in an area of the light-emitting wavelength of the fluorescent printing material is low. a processing means configured to perform processing relating to color calibration in the printing apparatus that prints an image on a printing medium using a fluorescent printing material, wherein the fluorescent printing material is a color material that develops a color by entering an activated state by absorbing light having an activation wavelength from a ground state and returning to the ground state by emitting light having a light-emitting wavelength; and an execution means configured to perform calibration processing of the printing apparatus based on a measured value that is obtained by measuring a patch chart for obtaining information on an amount of application of the fluorescent printing material on the printing medium, wherein the printing apparatus is configured to print a patch included in the patch chart with the fluorescent printing material and a subtractive color mixture printing material so that at least one dot of the subtractive color mixture printing material covers a dot of the fluorescent printing material on the printing medium, wherein the subtractive color mixture printing material contains a color material that absorbs light having a specific wavelength and does not emit the light among cast light, and wherein the printing apparatus further comprises a first measuring unit for measuring the patch chart, the first measuring unit including one or a plurality of light sources and a light-receiving element and configured to measure the patch included in the patch chart, wherein the first measuring unit is configured to cast light of the light source whose spectral distribution exists in a wavelength area including activation wavelengths of the fluorescent printing material to the patch, characterized in that the processing relating to color calibration comprises: selecting a subtractive color mixture printing material as the subtractive color mixture printing material, whose spectral reflectance in an area of the activation wavelength of the fluorescent printing material is high and whose reflectance in an area of the light-emitting wavelength of the fluorescent printing material is low.