mhurhangee/ep-ind-claims huggingface dataset:
A method for refolding an insoluble protein or peptide that lacks essential disulfides and that comprises at least one free cysteine residue, comprising the following steps: a) causing a host cell to express a protein or peptide that lacks essential disulfides and that comprises at least one free cysteine residue in an insoluble or aggregated form; b) lysing the host cell; c) isolating the protein or peptide; d) denaturing and reducing the protein or peptide in a solution comprising both a denaturing agent and a reducing agent, wherein said reducing agent does not form a mixed disulfide with the free cysteine in the protein or peptide, and wherein said reducing agent does not inactivate a thiol-reactive polyethylene glycol or does not interfere with modification of the protein by a thiol-reactive PEG reagent; e) refolding the protein by reducing the concentrations of the denaturing agent and reducing agents in the solution of (d) to levels sufficient to allow the protein or peptide to renature into a soluble, biologically active form; wherein steps (d) and (e) occur in the absence of a cysteine blocking agent; and wherein the essential disulfides are disulfides that are essential in the proper folding and/or biological activity of the protein or peptide. a) causing a host cell to express a protein or peptide that lacks essential disulfides and that comprises at least one free cysteine residue in an insoluble or aggregated form; b) lysing the host cell; c) isolating the protein or peptide; d) denaturing and reducing the protein or peptide in a solution comprising both a denaturing agent and a reducing agent, wherein said reducing agent does not form a mixed disulfide with the free cysteine in the protein or peptide, and wherein said reducing agent does not inactivate a thiol-reactive polyethylene glycol or does not interfere with modification of the protein by a thiol-reactive PEG reagent; e) refolding the protein by reducing the concentrations of the denaturing agent and reducing agents in the solution of (d) to levels sufficient to allow the protein or peptide to renature into a soluble, biologically active form;
A fluid processing apparatus for processing a material to be processed between opposed processing surfaces (1,2) of processing members (10, 20) capable of approaching to and separating from each other, at least one of which rotates relative to the other, the apparatus comprising a first flow path through which a first fluid to be processed can be introduced into a space formed between the opposed processing surfaces to form a thin film of the fluid, wherein at least one of the processing members comprises a plurality of components (20a, 20b, 20c) of the processing member obtained by dividing the processing member into at least a first component and a second component, and the processing member is formed by assembling a plurality of these divided components (20a, 20b, 20c) of the processing member; wherein at least two fluids to be processed can be used, wherein the apparatus comprises a separate flow path having an opening to the space between the opposed processing surfaces, the said flow path being independent of the first flow path through which the first fluid to be processed can be introduced, such that a second fluid to be processed can be introduced into between the processing surfaces (1, 2) whereby the first and second fluids to be processed in use are mixed and stirred between the relatively rotating processing surfaces, characterized in that a space between the first component and second component of the plurality of components is made to be a part of said separate flow path having an opening leading to the space between the processing surfaces.
A method in a wireless terminal for enabling feedback in an uplink transmission of a communication system from the wireless terminal to a base station, the method comprising: establishing a dedicated feedback channel between the wireless terminal and the base station; the wireless terminal autonomously transmitting a pre-reserved feedback payload code to the base station on the dedicated feedback channel as an intention to send Mobile Subscriber Station, MSS, feedback information from the wireless terminal to the base station; receiving, from the base station, an information element within a data frame wherein the information element a indicates an uplink resource for transmitting MSS feedback information in a Media Access Control, MAC, feedback protocol data unit, PDU, the uplink resource received in response to the pre-reserved feedback payload code transmitted by the wireless terminal that the wireless terminal has MSS feedback information to transmit; and transmitting, to the base station, Mobile Subscriber Station, MSS, feedback information in a MAC feedback PDU in the uplink resource which is indicated in the information element, the MSS feedback information comprising feedback type including a Multiple-Input and Multiple-Output, MIMO, mode or permutation mode feedback type, feedback content and a connection identifier, CID. establishing a dedicated feedback channel between the wireless terminal and the base station; the wireless terminal autonomously transmitting a pre-reserved feedback payload code to the base station on the dedicated feedback channel as an intention to send Mobile Subscriber Station, MSS, feedback information from the wireless terminal to the base station; receiving, from the base station, an information element within a data frame wherein the information element a indicates an uplink resource for transmitting MSS feedback information in a Media Access Control, MAC, feedback protocol data unit, PDU, the uplink resource received in response to the pre-reserved feedback payload code transmitted by the wireless terminal that the wireless terminal has MSS feedback information to transmit; and transmitting, to the base station, Mobile Subscriber Station, MSS, feedback information in a MAC feedback PDU in the uplink resource which is indicated in the information element, the MSS feedback information comprising feedback type including a Multiple-Input and Multiple-Output, MIMO, mode or permutation mode feedback type, feedback content and a connection identifier, CID.
Aqueous composition for colouring keratin fibres especially human hair, based on at least one oxidative dye precursor, optionally at least one coupling agent and/or at least one direct dye in a cosmetically acceptable medium characterised in that it comprises at least one anionic surfactant, at least one branched fatty acid and at least one quaternary ammonium surfactant selected from compounds according to general structure R 3 -A-R 4 -B wherein R 3 is a saturated or unsaturated, straight or branched alkyl group with 8 to 24 C atoms, R 4 is a straight or branched alkyl group with 1 to 4 C atoms, A is a group selected from O, and and B is R 5, and R 6 are the same or different, an alkyl with 1 to 4 C atoms, hydroxyl alkyl with 1 to 4 C atoms and di hydroxyl alkyl with 2 to 4 C atoms, R 7 is analkyl with 1 to 4 C atoms, hydroxyl_alkyl with 1 to 4 C atoms or di hydroxyl alkylwith 2 to 4 C atoms and -R 4 -A-R 3 wherein R 3, A and R 4 have the above meaning and X is chloride bromide, methosulfate and where R 8 and R 9 are a saturated or unsaturated, branched or straight alkyl chain with 8-24 C atoms, and R 10 and R 11 are independent from each other lower alkyl chain with 1 to 4 carbon atoms which may be substituted with one or two hydroxyl group, and X is chloride, bromide or methosulfate.
An implant for covering bone defects, in particular in a jaw region, wherein the implant consists of a magnesium film, wherein the magnesium film has a thickness between 70 and 200 pm, characterized in that the magnesium film has no openings, at least in a central area thereof.
A porous, bioabsorbable nerve tube structure comprising: a bioabsorbable, polymeric tubular foam member comprising a lumen, an inner surface surrounding the lumen and an outer surface defining a wall, the wall having a thickness, wherein the substrate has a gradient of pore size from the interior surface to the exterior surface across the wall thickness of the tubular member such that tissue cells may infiltrate from the inner surface into the wall, and the outer surface is impermeable by cells, and the substrate is permeable by fluids through the wall of the foam member; wherein the gradient of pore size comprises pores each having a diameter, the diameters ranging along the gradient from about 100 μm at the inner surface of the wall to about 1 μm at the outer surface of the wall.
An ink delivery system for delivering ink to one or more inkjet printheads, the system comprising: a first ink tank having a first air port, a first ink port for connection to a printhead outlet, an ink refill port for connection to a bulk ink supply and a first ink circulation port; a second ink tank having a second air port, a second ink port for connection to a printhead inlet and a second ink circulation port; a main air line having an air inlet and an air outlet; a first air line interconnecting the first air port and the main air line; a second air line interconnecting the second air port and the main air line; an air pump positioned in the main air line between the first and second air lines, the air pump being configured for pumping air from the air inlet towards the air outlet during printing; a first airflow restrictor positioned between the air inlet and the first air line; a second airflow restrictor positioned between the air outlet and the second air line; an ink circulation line interconnecting the first and second ink tanks: an ink pump positioned in the ink circulation line for pumping ink from the first ink tank to the second ink tank, and characterized by: a controller configured for adjusting, during printing, the first and second air flow restrictors, thereby controlling first and second ink pressures in the first and second ink tanks. a first ink tank having a first air port, a first ink port for connection to a printhead outlet, an ink refill port for connection to a bulk ink supply and a first ink circulation port; a second ink tank having a second air port, a second ink port for connection to a printhead inlet and a second ink circulation port; a main air line having an air inlet and an air outlet; a first air line interconnecting the first air port and the main air line; a second air line interconnecting the second air port and the main air line; an air pump positioned in the main air line between the first and second air lines, the air pump being configured for pumping air from the air inlet towards the air outlet during printing; a first airflow restrictor positioned between the air inlet and the first air line; a second airflow restrictor positioned between the air outlet and the second air line; an ink circulation line interconnecting the first and second ink tanks: an ink pump positioned in the ink circulation line for pumping ink from the first ink tank to the second ink tank, and characterized by: a controller configured for adjusting, during printing, the first and second air flow restrictors, thereby controlling first and second ink pressures in the first and second ink tanks.
A smart card comprising: a data memory arranged to store data, a write unit (21, S55) arranged to write data to be written to the data memory according to a write command supplied from an external device, a determining unit (21, S52) arranged to determine whether important data is contained in the data to be written specified by the write command supplied from the external device, a calculating unit (21, S56) arranged to calculate a head address of the important data in the data memory based on a data length from a head of the data to be written to a head of the important data and based on an address on the data memory at which a process of writing the data to be written is started when the determining unit determines that important data is contained in the data to be written, and a position table arranged to store information indicating the calculated head address of the important data in correspondence to information indicating the important data, wherein the write command requests writing of data with a nested structure, the important data being child data with high frequency of use as preset child data among a plurality of pieces of the child data included in data with the nested structure. a data memory arranged to store data, a write unit (21, S55) arranged to write data to be written to the data memory according to a write command supplied from an external device, a determining unit (21, S52) arranged to determine whether important data is contained in the data to be written specified by the write command supplied from the external device, a calculating unit (21, S56) arranged to calculate a head address of the important data in the data memory based on a data length from a head of the data to be written to a head of the important data and based on an address on the data memory at which a process of writing the data to be written is started when the determining unit determines that important data is contained in the data to be written, and a position table arranged to store information indicating the calculated head address of the important data in correspondence to information indicating the important data, wherein the write command requests writing of data with a nested structure, the important data being child data with high frequency of use as preset child data among a plurality of pieces of the child data included in data with the nested structure.
An underbody panelling part of a wheel axle in the region of the wheel arch of a motor vehicle, with a main body, which is fastened to the vehicle, characterised in that the covering surface of the main body is variable, and in that the main body has a fastening structure for fixed connection to the vehicle body and/or a fastening structure for fixed connection to a transverse link of the vehicle.
An instrument connection device, in particular for instruments that require a gas supply and a power supply, having a gas junction, which comprises a hollow cylindrical body with a flexible wall that delimits a through channel and that comprises a hole, having a hose, which comprises at least one lumen and is arranged with one end extending in proximal direction into the body, in abutment against the wall, having a wire, which is arranged in the hose projecting in proximal direction therefrom and which extends through the hole, characterized in that the hole is a punctured hole which is elastically widened by the wire and that the flexible wall abuts against the wire at the punctured hole in an elastically tensioned manner. having a gas junction, which comprises a hollow cylindrical body with a flexible wall that delimits a through channel and that comprises a hole, having a hose, which comprises at least one lumen and is arranged with one end extending in proximal direction into the body, in abutment against the wall, having a wire, which is arranged in the hose projecting in proximal direction therefrom and which extends through the hole, characterized in that the hole is a punctured hole which is elastically widened by the wire and that the flexible wall abuts against the wire at the punctured hole in an elastically tensioned manner.
An umbilicus for use in a blood processing system comprising a centrifuge assembly, comprising: an elongated umbilicus body including first and second ends (82, 84) and an intermediate section located there between, wherein the umbilicus body defines at least one fluid-transmitting lumen extending between the first and second ends (82, 84); and a first end fitting defining an interior cavity, wherein the first end of the umbilicus body is enlarged compared to the intermediate section, the interior cavity of the first end fitting includes a flared portion having a non-circular cross-sectional shape, and at least a portion of the enlarged first end of the umbilicus body has a non-circular cross-sectional shape and is seated within the flared portion of the interior cavity of the first end fitting. an elongated umbilicus body including first and second ends (82, 84) and an intermediate section located there between, wherein the umbilicus body defines at least one fluid-transmitting lumen extending between the first and second ends (82, 84); and a first end fitting defining an interior cavity, wherein the first end of the umbilicus body is enlarged compared to the intermediate section, the interior cavity of the first end fitting includes a flared portion having a non-circular cross-sectional shape, and at least a portion of the enlarged first end of the umbilicus body has a non-circular cross-sectional shape and is seated within the flared portion of the interior cavity of the first end fitting.
A wind turbine comprising: a wind turbine rotor with a plurality of blades supported on a support structure; a generator operatively coupled to the wind turbine rotor for generating electrical power; a power electronic converter for converting electrical power generated by the generator to a converted AC power of predetermined frequency and voltage; a main wind turbine transformer having a low voltage side and a high voltage side for transforming the converted AC power to a higher voltage; characterized in that one or more electric filters are connected to the high voltage side of the main transformer, and the electric filters are arranged in the support structure. a wind turbine rotor with a plurality of blades supported on a support structure; a generator operatively coupled to the wind turbine rotor for generating electrical power; a power electronic converter for converting electrical power generated by the generator to a converted AC power of predetermined frequency and voltage; a main wind turbine transformer having a low voltage side and a high voltage side for transforming the converted AC power to a higher voltage; characterized in that one or more electric filters are connected to the high voltage side of the main transformer, and the electric filters are arranged in the support structure.
Fuel injection valve for fuel injection systems of internal combustion engines, in particular for the direct injection of fuel into the combustion chamber of an internal combustion engine, with an actuator, by way of the excitation of which a stroke movement of a valve needle can be achieved, as a result of which an actuation of a valve closing body which forms a sealing seat together with a valve seat face is made possible, and with a feed-side feed connecting piece for a fuel feed, a radial supporting disc for captive securing of the fuel injection valve in a mounted state being arranged at the feed-side end of the feed connecting piece, and a sealing ring which surrounds the feed connecting piece being provided on the latter, and the radial supporting disc still being arranged upstream of the sealing ring as viewed in the flow direction, characterized in that an end collar is shaped at the feed-side end of the feed connecting piece, and the radial supporting disc engages over and under this end collar at least partially. a radial supporting disc for captive securing of the fuel injection valve in a mounted state being arranged at the feed-side end of the feed connecting piece, and a sealing ring which surrounds the feed connecting piece being provided on the latter, and the radial supporting disc still being arranged upstream of the sealing ring as viewed in the flow direction, characterized in that an end collar is shaped at the feed-side end of the feed connecting piece, and the radial supporting disc engages over and under this end collar at least partially.
A method comprising: generating one index that integrates a local file index and a local application content search index, wherein the local file index represents a collection of scanned and parsed data from local files for use in identifying search results satisfying a search query, and wherein the application content search index represents a collection of parsed data from an application database or other application content datastore; receiving at least a portion of a search query by a search service; searching by the search service the integrated index based on the at least one portion of the search query; obtaining, based on the searching of the integrated index, one or more contextualized search results associated with application content, the one or more contextualized search results indicating the application to be used to access the application content; and returning from the search service the one or more contextual search results responsive to the searching operation, each contextual search result including a reference to content associated with the search result and an application identifier identifying the application; receiving a selection of a selected contextual search result from the one or more contextual search results; and launching the application identified by the application identifier of the selected contextual search result to operate on the content associated with the selected contextual search result. generating one index that integrates a local file index and a local application content search index, wherein the local file index represents a collection of scanned and parsed data from local files for use in identifying search results satisfying a search query, and wherein the application content search index represents a collection of parsed data from an application database or other application content datastore; receiving at least a portion of a search query by a search service; searching by the search service the integrated index based on the at least one portion of the search query; obtaining, based on the searching of the integrated index, one or more contextualized search results associated with application content, the one or more contextualized search results indicating the application to be used to access the application content; and returning from the search service the one or more contextual search results responsive to the searching operation, each contextual search result including a reference to content associated with the search result and an application identifier identifying the application; receiving a selection of a selected contextual search result from the one or more contextual search results; and launching the application identified by the application identifier of the selected contextual search result to operate on the content associated with the selected contextual search result.