Microscope adapted for working in digital holography comprising: - a partially coherent light source, capable of generating a source light beam; - a first mobile subassembly, comprising a beam separator and a mirror, in order to form two parallel beams, an object beam and a reference beam from the source light beam; - a second subassembly, also comprising a beam separator and a mirror, in order to combine said object beam and said reference beam into a single recombined light beam; - an object cell (So) comprising a sample to be studied, positioned on the optical path of the object beam between the first subassembly and the second subassembly; - a first microscope objective, on the optical path of the object beam between the first subassembly and the second subassembly, downstream from the object cell (So); - a second microscope objective, positioned on the optical path of the reference beam between the first subassembly and the second subassembly; - recording means for recording interferometric signals produced by the recombined light beam; - additional focusing means for focusing said recombined light beam on said recording means, said recording means being located in the rear focal plane of the additional focusing means, allowing an adjustment of the second subassembly without altering the focus on said recording means; - optical path compensating means; - first focusing means able to focus the object beam on the object cell (So) and second focusing means able to focus the reference beam on said optical path compensating means in which the first focusing means form, with the first microscope objective, an afocal system and the second focusing means form, with the second microscope objective, an afocal system. - a partially coherent light source, capable of generating a source light beam; - a first mobile subassembly, comprising a beam separator and a mirror, in order to form two parallel beams, an object beam and a reference beam from the source light beam; - a second subassembly, also comprising a beam separator and a mirror, in order to combine said object beam and said reference beam into a single recombined light beam; - an object cell (So) comprising a sample to be studied, positioned on the optical path of the object beam between the first subassembly and the second subassembly; - a first microscope objective, on the optical path of the object beam between the first subassembly and the second subassembly, downstream from the object cell (So); - a second microscope objective, positioned on the optical path of the reference beam between the first subassembly and the second subassembly; - recording means for recording interferometric signals produced by the recombined light beam; - additional focusing means for focusing said recombined light beam on said recording means, said recording means being located in the rear focal plane of the additional focusing means, allowing an adjustment of the second subassembly without altering the focus on said recording means; - optical path compensating means; - first focusing means able to focus the object beam on the object cell (So) and second focusing means able to focus the reference beam on said optical path compensating means in which the first focusing means form, with the first microscope objective, an afocal system and the second focusing means form, with the second microscope objective, an afocal system.