A multi-channel microfluidic blood coagulation detection chip, comprising a chip body, wherein the chip body comprises, in sequence from bottom to top, a lower-layer chip, a middle-layer chip, and an upper-layer chip; the lower-layer chip, the middle-layer chip, and the upper-layer chip cooperate with each other to define a closed microfluidic channel and a plurality of mutually independent detection chambers, the upper-layer chip is provided with a sample loading hole, and the sample loading hole communicates with the detection chambers through the microfluidic channel; the multi-channel microfluidic blood coagulation detection chip is characterized in that the chip body further comprises electrodes, the electrodes comprise upper-layer electrodes and lower-layer electrodes, the upper-layer electrodes are disposed on the back surface of the upper-layer chip, the lower-layer electrodes are disposed on the front surface of the lower-layer chip, and a gap is provided between the upper-layer electrodes and the lower-layer electrodes, wherein both the upper-layer electrodes on the back surface of the upper-layer chip and the lower-layer electrodes on the front surface of the lower-layer chip are arranged corresponding to positions where the detection chambers of the middle-layer chip are located, and the upper-layer electrodes and the lower-layer electrodes communicate with each other through the detection chambers, wherein the middle-layer chip is provided with middle-layer communication holes at positions corresponding to the upper-layer electrodes, the lower-layer chip is provided with lower-layer communication holes at positions corresponding to the upper-layer electrodes, and the upper-layer electrodes are in contact and connection with a detection instrument through the middle-layer communication holes and the lower-layer communication holes, wherein both the microfluidic channel and the detection chambers run through the middle-layer chip; the microfluidic channel comprises a main flow channel and a plurality of branching microfluidic channels, the tail end of the main flow channel is divided into the plurality of branching microfluidic channels, and the plurality of branching microfluidic channels communicate in one-to-one correspondence with the plurality of mutually independent detection chambers; and the front end of the main flow channel communicates with the sample loading hole, and wherein the upper-layer electrodes comprise a plurality of electrodes I, and the plurality of electrodes I are all arranged in one-to-one correspondence with the plurality of mutually independent detection chambers; the lower-layer electrodes comprise a plurality of electrodes II, the plurality of electrodes II are all arranged in one-to-one correspondence with the plurality of mutually independent detection chambers, and the electrodes I and the electrodes II are respectively working electrodes or reference electrodes. wherein both the upper-layer electrodes on the back surface of the upper-layer chip and the lower-layer electrodes on the front surface of the lower-layer chip are arranged corresponding to positions where the detection chambers of the middle-layer chip are located, and the upper-layer electrodes and the lower-layer electrodes communicate with each other through the detection chambers, wherein the middle-layer chip is provided with middle-layer communication holes at positions corresponding to the upper-layer electrodes, the lower-layer chip is provided with lower-layer communication holes at positions corresponding to the upper-layer electrodes, and the upper-layer electrodes are in contact and connection with a detection instrument through the middle-layer communication holes and the lower-layer communication holes, wherein both the microfluidic channel and the detection chambers run through the middle-layer chip; the microfluidic channel comprises a main flow channel and a plurality of branching microfluidic channels, the tail end of the main flow channel is divided into the plurality of branching microfluidic channels, and the plurality of branching microfluidic channels communicate in one-to-one correspondence with the plurality of mutually independent detection chambers; and the front end of the main flow channel communicates with the sample loading hole, and wherein the upper-layer electrodes comprise a plurality of electrodes I, and the plurality of electrodes I are all arranged in one-to-one correspondence with the plurality of mutually independent detection chambers; the lower-layer electrodes comprise a plurality of electrodes II, the plurality of electrodes II are all arranged in one-to-one correspondence with the plurality of mutually independent detection chambers, and the electrodes I and the electrodes II are respectively working electrodes or reference electrodes.