A method of preparing a Master Cell Bank composition comprising Mesenchymal stem cells, Fetal Bovine Serum and Dimethyl Sulphoxide (DMSO), said method comprising acts of: a. diluting obtained bone marrow cells in culture media comprising Dulbecco's Modified Eagle Medium-KnockOut [DMEM-KO], Fetal Bovine Serum, Glutamine and Pen-Strep and centrifuging the diluted bone marrow to obtain a pellet and re-diluting the pellet with the culture media; b. adding density gradient solution to the re-diluted pellet and centrifuging to obtain a buffy layer at interface; c. to the buffy layer re-adding equal volume of the culture media and centrifuging to obtain a pellet comprising Mononuclear cells, followed by suspending the pellet in the culture media; seeding about 40 - 50 million Mononuclear cells and culturing with media change to achieve about 80% to 85% confluency; d. aspirating the culture media and washing the confluent cells with Dulbecco's Phosphate Buffered Saline (DPBS), thereafter treating the cells with 0.25% trypsin to obtain trypsinized cells; e. neutralizing the trypsinized cells with neutralization media and re- centrifuging the cells to obtain a pellet comprising Mesenchymal Stem Cells, followed by suspending the pellet in the culture media; f. seeding the Mesenchymal Stem Cells at a seeding density of about 6666 cells/cm 2 and culturing with media change to achieve about 80 % to 85 % confluency; g. harvesting the cells of step f) by aspirating the culture media, washing the confluent cells with DPBS, trypsinizing the cells, neutralizing the trypsinized cells with Neutralization media and centrifuging to obtain pellet comprising Mesenchymal Stem Cells; and h. suspending the Mesenchymal Stem Cells infreezing media comprising about 85% to 95% FBS and about 5% to 15% DMSO to obtain the Master Cell Bank composition. a. diluting obtained bone marrow cells in culture media comprising Dulbecco's Modified Eagle Medium-KnockOut [DMEM-KO], Fetal Bovine Serum, Glutamine and Pen-Strep and centrifuging the diluted bone marrow to obtain a pellet and re-diluting the pellet with the culture media; b. adding density gradient solution to the re-diluted pellet and centrifuging to obtain a buffy layer at interface; c. to the buffy layer re-adding equal volume of the culture media and centrifuging to obtain a pellet comprising Mononuclear cells, followed by suspending the pellet in the culture media; seeding about 40 - 50 million Mononuclear cells and culturing with media change to achieve about 80% to 85% confluency; d. aspirating the culture media and washing the confluent cells with Dulbecco's Phosphate Buffered Saline (DPBS), thereafter treating the cells with 0.25% trypsin to obtain trypsinized cells; e. neutralizing the trypsinized cells with neutralization media and re- centrifuging the cells to obtain a pellet comprising Mesenchymal Stem Cells, followed by suspending the pellet in the culture media; f. seeding the Mesenchymal Stem Cells at a seeding density of about 6666 cells/cm 2 and culturing with media change to achieve about 80 % to 85 % confluency; g. harvesting the cells of step f) by aspirating the culture media, washing the confluent cells with DPBS, trypsinizing the cells, neutralizing the trypsinized cells with Neutralization media and centrifuging to obtain pellet comprising Mesenchymal Stem Cells; and h. suspending the Mesenchymal Stem Cells infreezing media comprising about 85% to 95% FBS and about 5% to 15% DMSO to obtain the Master Cell Bank composition.