A method for determining an analyte suspected to be present in a sample comprising: (a) contacting said sample with (i) a first binding agent which is capable of specifically binding to the analyte and which is immobilized on a solid support; and (ii) a second binding agent which is capable of specifically binding to the analyte and which is capable of reversibly binding at least one detectable label; wherein said first and/or second binding agent is linked to an linking agent which is capable of covalently binding the at least one reversibly bound detectable label to the solid support when the first and second binding agents are in physical proximity, for a time and under conditions which allow: - specific binding of the analyte to the first and the second binding agent such that an complex of first binding agent, analyte and second binding agent is formed; and - which allow for covalently binding the at least one detectable label to the solid support; and (b) removing the complex of first binding agent, analyte and second binding agent from the solid support such that the solid support having covalently bound the at least one detectable label remains; and (c) detecting the at least one covalently bound detectable label on the solid support whereby the analyte is determined. (a) contacting said sample with (i) a first binding agent which is capable of specifically binding to the analyte and which is immobilized on a solid support; and (ii) a second binding agent which is capable of specifically binding to the analyte and which is capable of reversibly binding at least one detectable label; wherein said first and/or second binding agent is linked to an linking agent which is capable of covalently binding the at least one reversibly bound detectable label to the solid support when the first and second binding agents are in physical proximity, for a time and under conditions which allow: - specific binding of the analyte to the first and the second binding agent such that an complex of first binding agent, analyte and second binding agent is formed; and - which allow for covalently binding the at least one detectable label to the solid support; and (i) a first binding agent which is capable of specifically binding to the analyte and which is immobilized on a solid support; and (ii) a second binding agent which is capable of specifically binding to the analyte and which is capable of reversibly binding at least one detectable label; wherein said first and/or second binding agent is linked to an linking agent which is capable of covalently binding the at least one reversibly bound detectable label to the solid support when the first and second binding agents are in physical proximity, for a time and under conditions which allow: - specific binding of the analyte to the first and the second binding agent such that an complex of first binding agent, analyte and second binding agent is formed; and - which allow for covalently binding the at least one detectable label to the solid support; and - specific binding of the analyte to the first and the second binding agent such that an complex of first binding agent, analyte and second binding agent is formed; and - which allow for covalently binding the at least one detectable label to the solid support; and (b) removing the complex of first binding agent, analyte and second binding agent from the solid support such that the solid support having covalently bound the at least one detectable label remains; and (c) detecting the at least one covalently bound detectable label on the solid support whereby the analyte is determined.