A variant of a parent protease, wherein the parent protease is subtilisin 309 (SEQ ID NO: 2), wherein the variant consists of SEQ ID NO: 2 with one of the alterations selected from the group consisting of: S9R+A15T+T22TW+N204D+Q245I; S9R+A15T+G97GG+P131S+Q137H; S9R+A15T+T22TG+N62D+V139L+Q245G; S9R+A15T+V68A+Q245R; S9R+A15T+G97GA+H120N+S212L; S9R+A15T+L96LG+H120D+P131H+R186L; S9R+A15T+G97GA+H120D+Q137D; S9R+A15T+A16P+G97GA+P131S+Q137D+N204S; S9R+A15T+L21LP+T22TV+M119I+N218D+Q245I; S9R+A15T+G97GV+H120D+Q137H; S9R+A15T+L96LG+H120N+P131H+Q137E; S9R+A15T+L96LG+H120D+P131S+Q137E; S9R+A15T+H120N+P131T+N218D; V4A+S9R+A15T+G97GV+H120D; S9R+A15T+L96LG+H120D+G160D; S9R+A15T+T22TG+N62D+V139L+Q245S; S9R+A15T+G61E+A85T+P239L+Q245C; S9R+A15T+P131H+S144P; S9R+A15T+G97GA+Q137E; S9R+A15T+G97GA+H120Q+P131H+Q137E; S9R+A15T+L21LW+G100S+V139L+Q245V; S9R+A15T+G97GA+Q137H+N218S; S9R+A15T+L96LG+H120N+P131S+Q137H; S9R+A15T+G97GA+H120N+Q137E; S9R+A15T+L96LG+P131T+Q137H; S9R+A15T+L96LG+H120N+P131S; S9R+A15T+V68A+Q137D; S9R+A15T+G97GA+H120Y+Q137H; S9R+A15T+G97GA+Q137D; S9R+A15T+K94N+H120N+P131H; S9R+A15T+L96LG+P131H+Q137D; S9R+A15T+F50S+H120D+P131H; S9R+A15T+G97GA+H120N+Q137D+N248D; S9R+A15T+L96LG+P131Q+Q137D; S9R+A15T+T22G+V139L+Q245L; S9R+A15T+Q245F; S9R+A15T+Q245S; S9R+A15T+G97GV+H120Q; S9R+A15T+G97GA+Q137E+L262V; S9R+A15T+G127E+P131R+Q137H; S9R+A13V+A15T+I35V+N62D+Q245F; S9R+A15T+Q245V; S9R+A15T+T22A+V139L+Q245E; S9R+A15T+T22L+V139L+Q245V+A254S; S9R+A15T+Q245L; S9R+A15T+G61E+A85T+P239S+Q245V; S9R+A15T+G61E+A85T+Q206L+Q245R; S9R+A1 5T+N62NG+Q245T; S9R+A15T+G61GP+Q245L; S9R+A15T+G61E+A85T+P239S+Q245C; S9R+A15T+G102S+M175T+Q245R+N252D; S9R+A1 5T+N62D+Q245W+N252V; S9R+A15T+N62D+Q245R+N252M; S9R+A1 5T+N62D+Q245W+N252S; S9R+A15T+T22A+N62D; S9R+A15T+T22TQ+S101P; S9R+A15T+H120R+Q137D+N173S; S9R+A15T+L96LG+Q137E+Y209H; S9R+A15T+L111V+Q137E+G211D; S9R+A15T+L111I+Q137E; S9R+A15T+L111I+H120N+Q137E; S9R+A15T+L96LG+H120Q+Q137E; S9R+A15T+T260M; S9R+A15T; S9R+A15T+H120G+Q137E+N218D; S9R+A15T+S130P; S9R+A15T+N218D; S9R+A15T+T224A; S9R+A15T+D60DG; S9R+A15T+N62D+H120N+P131T; S9R+A15T+V68A+N218D+Q245R; S9R+A15T+V68A+H120N+N218D+Q245R; S9R+A15T+V68A+A174V+Q245R; S9R+A15T+G46D+V68A+N218D+Q245R; S9R+A15T+V68A+A98M+Q245R+N248D; S9R+A15T+V68A+A98L+S99G+Q245R; S9R+A15T+V68A+S99G+Q245R+N261D; S9R+A15T+N62S+H120N+P131T+N218D; S9R+A15T+H120N+P131T+N218D+N269T; S9R+A15T+L96LG+H120N+P131S+Q137H+M222S; S9R+A15T+G61E+A98S+S99M+Q245R: S9R+A15T+V68A+A98L +Q245R; S9R+A15T+V68A+A98G+S99V+Q245R; S9R+A15T+V68A+Q245R+N252S; S9R+A15T+V68A; S9R+A15T+Y167I+R170L; S9R+A15T+V68A+A194T+Q245R+N252E; S9R+A15T+V28I+V68A+Q245R+N252A; S9R+A15T+V68A+H120N+P131S+Q137H+Q245M; S9R+A15T+V68A+A98M+S99G+Q245R+T274A; and S9R+A15T+V68A+H120D+ P131S+Q137H+Q245R, wherein each position corresponds to a position of the amino acid sequence of subtilisin BPN', shown in SEQ ID NO: 1, and wherein the variant has protease activity. S9R+A15T+T22TW+N204D+Q245I; S9R+A15T+G97GG+P131S+Q137H; S9R+A15T+T22TG+N62D+V139L+Q245G; S9R+A15T+V68A+Q245R; S9R+A15T+G97GA+H120N+S212L; S9R+A15T+L96LG+H120D+P131H+R186L; S9R+A15T+G97GA+H120D+Q137D; S9R+A15T+A16P+G97GA+P131S+Q137D+N204S; S9R+A15T+L21LP+T22TV+M119I+N218D+Q245I; S9R+A15T+G97GV+H120D+Q137H; S9R+A15T+L96LG+H120N+P131H+Q137E; S9R+A15T+L96LG+H120D+P131S+Q137E; S9R+A15T+H120N+P131T+N218D; V4A+S9R+A15T+G97GV+H120D; S9R+A15T+L96LG+H120D+G160D; S9R+A15T+T22TG+N62D+V139L+Q245S; S9R+A15T+G61E+A85T+P239L+Q245C; S9R+A15T+P131H+S144P; S9R+A15T+G97GA+Q137E; S9R+A15T+G97GA+H120Q+P131H+Q137E; S9R+A15T+L21LW+G100S+V139L+Q245V; S9R+A15T+G97GA+Q137H+N218S; S9R+A15T+L96LG+H120N+P131S+Q137H; S9R+A15T+G97GA+H120N+Q137E; S9R+A15T+L96LG+P131T+Q137H; S9R+A15T+L96LG+H120N+P131S; S9R+A15T+V68A+Q137D; S9R+A15T+G97GA+H120Y+Q137H; S9R+A15T+G97GA+Q137D; S9R+A15T+K94N+H120N+P131H; S9R+A15T+L96LG+P131H+Q137D; S9R+A15T+F50S+H120D+P131H; S9R+A15T+G97GA+H120N+Q137D+N248D; S9R+A15T+L96LG+P131Q+Q137D; S9R+A15T+T22G+V139L+Q245L; S9R+A15T+Q245F; S9R+A15T+Q245S; S9R+A15T+G97GV+H120Q; S9R+A15T+G97GA+Q137E+L262V; S9R+A15T+G127E+P131R+Q137H; S9R+A13V+A15T+I35V+N62D+Q245F; S9R+A15T+Q245V; S9R+A15T+T22A+V139L+Q245E; S9R+A15T+T22L+V139L+Q245V+A254S; S9R+A15T+Q245L; S9R+A15T+G61E+A85T+P239S+Q245V; S9R+A15T+G61E+A85T+Q206L+Q245R; S9R+A1 5T+N62NG+Q245T; S9R+A15T+G61GP+Q245L; S9R+A15T+G61E+A85T+P239S+Q245C; S9R+A15T+G102S+M175T+Q245R+N252D; S9R+A1 5T+N62D+Q245W+N252V; S9R+A15T+N62D+Q245R+N252M; S9R+A1 5T+N62D+Q245W+N252S; S9R+A15T+T22A+N62D; S9R+A15T+T22TQ+S101P; S9R+A15T+H120R+Q137D+N173S; S9R+A15T+L96LG+Q137E+Y209H; S9R+A15T+L111V+Q137E+G211D; S9R+A15T+L111I+Q137E; S9R+A15T+L111I+H120N+Q137E; S9R+A15T+L96LG+H120Q+Q137E; S9R+A15T+T260M; S9R+A15T; S9R+A15T+H120G+Q137E+N218D; S9R+A15T+S130P; S9R+A15T+N218D; S9R+A15T+T224A; S9R+A15T+D60DG; S9R+A15T+N62D+H120N+P131T; S9R+A15T+V68A+N218D+Q245R; S9R+A15T+V68A+H120N+N218D+Q245R; S9R+A15T+V68A+A174V+Q245R; S9R+A15T+G46D+V68A+N218D+Q245R; S9R+A15T+V68A+A98M+Q245R+N248D; S9R+A15T+V68A+A98L+S99G+Q245R; S9R+A15T+V68A+S99G+Q245R+N261D; S9R+A15T+N62S+H120N+P131T+N218D; S9R+A15T+H120N+P131T+N218D+N269T; S9R+A15T+L96LG+H120N+P131S+Q137H+M222S; S9R+A15T+G61E+A98S+S99M+Q245R: S9R+A15T+V68A+A98L +Q245R; S9R+A15T+V68A+A98G+S99V+Q245R; S9R+A15T+V68A+Q245R+N252S; S9R+A15T+V68A; S9R+A15T+Y167I+R170L; S9R+A15T+V68A+A194T+Q245R+N252E; S9R+A15T+V28I+V68A+Q245R+N252A; S9R+A15T+V68A+H120N+P131S+Q137H+Q245M; S9R+A15T+V68A+A98M+S99G+Q245R+T274A; and S9R+A15T+V68A+H120D+ P131S+Q137H+Q245R, S9R+A15T+V68A+A98L +Q245R; S9R+A15T+V68A+A98G+S99V+Q245R; S9R+A15T+V68A+Q245R+N252S; S9R+A15T+V68A; S9R+A15T+Y167I+R170L; S9R+A15T+V68A+A194T+Q245R+N252E; S9R+A15T+V28I+V68A+Q245R+N252A; S9R+A15T+V68A+H120N+P131S+Q137H+Q245M; S9R+A15T+V68A+A98M+S99G+Q245R+T274A; and S9R+A15T+V68A+H120D+ P131S+Q137H+Q245R,