Method for manufacturing a microfluidic device comprising a microfluidic network comprising several microfluidic capsules in series, each microfluidic capsule having a chamber having a non-zero internal volume and which is arranged to control the flow of a fluid between an inlet channel and an outlet channel of the fluidic network, each microfluidic capsule being produced by the following steps: - producing a through-hole (102, 103) through a first card so as to define a cavity, said cavity having an inlet emerging on a first face of the first card, called top face, and an outlet emerging on a second face of the first card; - at least partially filling said cavity by the addition, via the inlet of said cavity, of a determined volume of an element made of deformable material initially taking the form of a liquid in order to delimit on the surface a deformable wall of said chamber and, opposite, a rear face of a command network of the capsule; - controlling said internal volume of the chamber by gauging the element made of deformable material added in the cavity to determine a concave form of said deformable wall and the depth of said wall; - solidifying said element made of deformable material inside the cavity so as to create said deformable wall delimiting said chamber, and in that the method also comprises steps of: - assembling a second card on said first card, so as to cover the inlet of each cavity, said second card comprising said microfluidic network comprising said inlet channel and said outlet channel associated with each capsule and arranged to communicate with the chamber of the capsule, - assembling a third card on said first card, so as to cover the outlet of said cavity, said third card comprising several actuation holes each emerging at a distinct microfluidic capsule. - producing a through-hole (102, 103) through a first card so as to define a cavity, said cavity having an inlet emerging on a first face of the first card, called top face, and an outlet emerging on a second face of the first card; - at least partially filling said cavity by the addition, via the inlet of said cavity, of a determined volume of an element made of deformable material initially taking the form of a liquid in order to delimit on the surface a deformable wall of said chamber and, opposite, a rear face of a command network of the capsule; - controlling said internal volume of the chamber by gauging the element made of deformable material added in the cavity to determine a concave form of said deformable wall and the depth of said wall; - solidifying said element made of deformable material inside the cavity so as to create said deformable wall delimiting said chamber, and in that the method also comprises steps of: - assembling a second card on said first card, so as to cover the inlet of each cavity, said second card comprising said microfluidic network comprising said inlet channel and said outlet channel associated with each capsule and arranged to communicate with the chamber of the capsule, - assembling a third card on said first card, so as to cover the outlet of said cavity, said third card comprising several actuation holes each emerging at a distinct microfluidic capsule.