A system for characterizing a biological sample by analyzing emission of fluorescent light from the biological sample upon excitation comprising: (i) a laser source connected to a biological sample via excitation fibers (ExF), wherein the laser is configured to radiate the biological sample with a laser pulse at a predetermined wavelength to cause the biological sample to produce a responsive fluorescence signal; (ii) collection fibers, wherein the collection fibers collect the fluorescence signal from the biological sample, and relays the fluorescence signal to a demultiplexer; (iii) a demultiplexer that is configured to split the fluorescence signal from the collection fibers at pre-determined wavelengths; and (iv) an optical delay device; characterized in that the demultiplexer is configured to split the fluorescence signal from the collection fibers at pre-determined wavelengths to obtain spectral bands of <365 nm, 365-410 nm, 410- 450 nm, 450-495nm, 500-560 nm, 560-600 nm and >600 nm, wherein the demultiplexer comprises a first wavelength splitting device at about >495 nm, a second wavelength splitting device at about 560 nm, a third wavelength splitting device at about 600 nm, a fourth wavelength splitting device at about 410 nm, a fifth wavelength splitting device at about 450 nm, and a sixth wavelength splitting device at about 365 nm, wherein the fluorescence signal from the collection fibers is split by the first wavelength splitting device into a <495nm signal and a >495 nm signal, the >495 nm signal is split by the second wavelength splitting device into a 500-560 nm signal and a >560 nm signal, the >560 nm signal is split by the third wavelength splitting device into a 560-600 nm signal and a >600 nm signal, the <495 nm signal is split by the fourth wavelength splitting device into a <410 nm signal and a 410-480nm signal, the 410-480 nm signal is split by the fifth wavelength splitting device into a 410-450 nm signal and a 450-495 nm signal, and the <410 nm signal is split by the sixth wavelength splitting device into a <365 nm signal and a 365-410 nm signal. (i) a laser source connected to a biological sample via excitation fibers (ExF), wherein the laser is configured to radiate the biological sample with a laser pulse at a predetermined wavelength to cause the biological sample to produce a responsive fluorescence signal; (ii) collection fibers, wherein the collection fibers collect the fluorescence signal from the biological sample, and relays the fluorescence signal to a demultiplexer; (iii) a demultiplexer that is configured to split the fluorescence signal from the collection fibers at pre-determined wavelengths; and (iv) an optical delay device; characterized in that the demultiplexer is configured to split the fluorescence signal from the collection fibers at pre-determined wavelengths to obtain spectral bands of <365 nm, 365-410 nm, 410- 450 nm, 450-495nm, 500-560 nm, 560-600 nm and >600 nm, wherein the demultiplexer comprises a first wavelength splitting device at about >495 nm, a second wavelength splitting device at about 560 nm, a third wavelength splitting device at about 600 nm, a fourth wavelength splitting device at about 410 nm, a fifth wavelength splitting device at about 450 nm, and a sixth wavelength splitting device at about 365 nm, wherein the fluorescence signal from the collection fibers is split by the first wavelength splitting device into a <495nm signal and a >495 nm signal, the >495 nm signal is split by the second wavelength splitting device into a 500-560 nm signal and a >560 nm signal, the >560 nm signal is split by the third wavelength splitting device into a 560-600 nm signal and a >600 nm signal, the <495 nm signal is split by the fourth wavelength splitting device into a <410 nm signal and a 410-480nm signal, the 410-480 nm signal is split by the fifth wavelength splitting device into a 410-450 nm signal and a 450-495 nm signal, and the <410 nm signal is split by the sixth wavelength splitting device into a <365 nm signal and a 365-410 nm signal.