A method of identifying a B cell that secretes an antigen-specific antibody, the method comprising: (a) harvesting B cells from an immunised rabbit host; (b) enriching the harvested rabbit B cells to increase the proportion of B cells that are specific for said antigen by affinity purification of antigen-specific B cells using an antigen directly or indirectly attached to a solid matrix or support, thereby forming an enriched B cell population; (c) culturing one or more sub-populations of cells from said enriched rabbit B cell population under conditions that favour the survival of a single B cell to produce a clonal population in at least one culture well; (d) determining whether said cultured sub-populations produce an antibody specific to said antigen, thereby identifying one or more antigen-positive sub-populations; and (e) determining whether a single rabbit B cell produces an antibody specific to the antigen by a method comprising: (i) isolating one or more single rabbit B cells from one or more of said antigen-positive sub-populations of step (d); (ii) providing an immobilized antigen comprising a matrix or solid support to which said antigen has been directly or indirectly attached; (iii) incubating an individual rabbit B cell with said immobilized antigen; (iv) incubating said immobilized antigen with a fluorophore labeled secondary antibody, wherein said secondary antibody is a host-specific anti-immunoglobulin antibody that binds antibodies of the host of step (a), thereby detecting whether an antibody secreted by said individual B cell is bound to said immobilized antigen; and (v) identifying a B cell that secretes an antibody specific to said antigen by a fluorimetric assay that detects antibody-antigen complexes formed adjacent to the secreting cell. (a) harvesting B cells from an immunised rabbit host; (b) enriching the harvested rabbit B cells to increase the proportion of B cells that are specific for said antigen by affinity purification of antigen-specific B cells using an antigen directly or indirectly attached to a solid matrix or support, thereby forming an enriched B cell population; (c) culturing one or more sub-populations of cells from said enriched rabbit B cell population under conditions that favour the survival of a single B cell to produce a clonal population in at least one culture well; (d) determining whether said cultured sub-populations produce an antibody specific to said antigen, thereby identifying one or more antigen-positive sub-populations; and (e) determining whether a single rabbit B cell produces an antibody specific to the antigen by a method comprising: (i) isolating one or more single rabbit B cells from one or more of said antigen-positive sub-populations of step (d); (ii) providing an immobilized antigen comprising a matrix or solid support to which said antigen has been directly or indirectly attached; (iii) incubating an individual rabbit B cell with said immobilized antigen; (iv) incubating said immobilized antigen with a fluorophore labeled secondary antibody, wherein said secondary antibody is a host-specific anti-immunoglobulin antibody that binds antibodies of the host of step (a), thereby detecting whether an antibody secreted by said individual B cell is bound to said immobilized antigen; and (v) identifying a B cell that secretes an antibody specific to said antigen by a fluorimetric assay that detects antibody-antigen complexes formed adjacent to the secreting cell. (i) isolating one or more single rabbit B cells from one or more of said antigen-positive sub-populations of step (d); (ii) providing an immobilized antigen comprising a matrix or solid support to which said antigen has been directly or indirectly attached; (iii) incubating an individual rabbit B cell with said immobilized antigen; (iv) incubating said immobilized antigen with a fluorophore labeled secondary antibody, wherein said secondary antibody is a host-specific anti-immunoglobulin antibody that binds antibodies of the host of step (a), thereby detecting whether an antibody secreted by said individual B cell is bound to said immobilized antigen; and (v) identifying a B cell that secretes an antibody specific to said antigen by a fluorimetric assay that detects antibody-antigen complexes formed adjacent to the secreting cell.