A method to grow endothelial cells with endothelial glycocalyx using a microfluidic chip (100; 500), comprising: seeding endothelial cells on the porous biocompatible membrane (113; 123); growing the endothelial cells to 80% or greater confluence on a surface of the porous biocompatible membrane (113; 123; 513) with a low flow rate of pumped cell culture medium in a CO 2 cell culture incubator (112; 114); increasing the flow rate of cell culture medium to a high flow rate to mimic blood flow shear stress in vivo to increase growth of an endothelial glycocalyx on the endothelial cells; and measuring at least one of a thickness and an integrity of the endothelial glycocalyx; wherein the microfluidic chip comprising: at least one multichamber flow assembly (110; 120) comprising: a plurality of microchannels (115; 125), wherein: a first microchannel of the plurality of microchannels (115; 125) includes: a first inlet (116 a-c); a first outlet (118 a-c); and a first chamber fluidly connected to the first inlet (116 a-c) and the first outlet (118 a-c); and a second microchannel of the plurality of microchannels includes: a second inlet (126a-c); a second outlet (128a-c); and a second chamber fluidly connected to the second inlet (126a-c) and the second outlet (128a-c); and a porous biocompatible membrane (113; 123) oriented along a longitudinal interface between the first microchannel and the second microchannel, wherein the porous biocompatible membrane (113; 123) is permeable for movement of biomolecules from the first chamber to the second chamber through the porous biocompatible membrane (113; 123), characterized in that a pump fluidly connected to at least one inlet (116a-c; 126a-c) and at least one outlet (118a-c; 128a-c) of one of the plurality of microchannels (115; 125) to circulate a cell culture medium through the at least one of the plurality of microchannels (115; 125). seeding endothelial cells on the porous biocompatible membrane (113; 123); growing the endothelial cells to 80% or greater confluence on a surface of the porous biocompatible membrane (113; 123; 513) with a low flow rate of pumped cell culture medium in a CO 2 cell culture incubator (112; 114); increasing the flow rate of cell culture medium to a high flow rate to mimic blood flow shear stress in vivo to increase growth of an endothelial glycocalyx on the endothelial cells; and measuring at least one of a thickness and an integrity of the endothelial glycocalyx; a plurality of microchannels (115; 125), wherein: a first microchannel of the plurality of microchannels (115; 125) includes: a first inlet (116 a-c); a first outlet (118 a-c); and a first chamber fluidly connected to the first inlet (116 a-c) and the first outlet (118 a-c); and a second microchannel of the plurality of microchannels includes: a second inlet (126a-c); a second outlet (128a-c); and a second chamber fluidly connected to the second inlet (126a-c) and the second outlet (128a-c); and a first microchannel of the plurality of microchannels (115; 125) includes: a first inlet (116 a-c); a first outlet (118 a-c); and a first chamber fluidly connected to the first inlet (116 a-c) and the first outlet (118 a-c); and a first inlet (116 a-c); a first outlet (118 a-c); and a first chamber fluidly connected to the first inlet (116 a-c) and the first outlet (118 a-c); and a second microchannel of the plurality of microchannels includes: a second inlet (126a-c); a second outlet (128a-c); and a second chamber fluidly connected to the second inlet (126a-c) and the second outlet (128a-c); and a second inlet (126a-c); a second outlet (128a-c); and a second chamber fluidly connected to the second inlet (126a-c) and the second outlet (128a-c); and a porous biocompatible membrane (113; 123) oriented along a longitudinal interface between the first microchannel and the second microchannel, wherein the porous biocompatible membrane (113; 123) is permeable for movement of biomolecules from the first chamber to the second chamber through the porous biocompatible membrane (113; 123), characterized in that a pump fluidly connected to at least one inlet (116a-c; 126a-c) and at least one outlet (118a-c; 128a-c) of one of the plurality of microchannels (115; 125) to circulate a cell culture medium through the at least one of the plurality of microchannels (115; 125).