A method for preparing a diene-based rubber latex comprising: a step of adding 50 parts by weight to 75 parts by weight of a conjugated diene-based monomer, 1 part by weight to 3 parts by weight of an emulsifier, 0.1 part by weight to 0.4 part by weight of a polymerization initiator, 0.1 part by weight to 3 parts by weight of an electrolyte, 0.1 part by weight to 0.5 part by weight of a molecular weight control agent and 65 parts by weight to 100 parts by weight of ion exchanged water, based on 100 parts by weight of the conjugated diene-based monomer, into a reactor, while stirring thereof, adding 0.05 part by weight to 0.3 part by weight of a cross-linking agent which is (propylene glycol) n diacrylate (wherein, n is 3 to 15), thereto, based on 100 parts by weight of the conjugated diene-based monomer, and then subjecting thereof to a first polymerization (Step 1); a step of adding the balance of the conjugated diene-based monomer, 0 to 0.1 part by weight of the emulsifier and 0 to 0.2 part by weight of the cross-linking agent at the time point that polymerization conversion rate of the (Step 1) is 45% to 60%, and then subjecting thereof to a second polymerization (Step 2); and a step of terminating the polymerization at the time point that the polymerization conversion rate of the (Step 2) is 90% to 95% or higher (Step 3), wherein the conjugated diene-based monomer is single material or a mixture, the mixture is composed of: 80 wt% to 99 wt% of a conjugated diene-based monomer; and 1 wt% to 20 wt% of at least one monomer selected from the group consisting of an aromatic vinyl-based monomer and a vinyl cyanide-based monomer. a step of adding 50 parts by weight to 75 parts by weight of a conjugated diene-based monomer, 1 part by weight to 3 parts by weight of an emulsifier, 0.1 part by weight to 0.4 part by weight of a polymerization initiator, 0.1 part by weight to 3 parts by weight of an electrolyte, 0.1 part by weight to 0.5 part by weight of a molecular weight control agent and 65 parts by weight to 100 parts by weight of ion exchanged water, based on 100 parts by weight of the conjugated diene-based monomer, into a reactor, while stirring thereof, adding 0.05 part by weight to 0.3 part by weight of a cross-linking agent which is (propylene glycol) n diacrylate (wherein, n is 3 to 15), thereto, based on 100 parts by weight of the conjugated diene-based monomer, and then subjecting thereof to a first polymerization (Step 1); a step of adding the balance of the conjugated diene-based monomer, 0 to 0.1 part by weight of the emulsifier and 0 to 0.2 part by weight of the cross-linking agent at the time point that polymerization conversion rate of the (Step 1) is 45% to 60%, and then subjecting thereof to a second polymerization (Step 2); and a step of terminating the polymerization at the time point that the polymerization conversion rate of the (Step 2) is 90% to 95% or higher (Step 3), wherein the conjugated diene-based monomer is single material or a mixture, the mixture is composed of: 80 wt% to 99 wt% of a conjugated diene-based monomer; and 1 wt% to 20 wt% of at least one monomer selected from the group consisting of an aromatic vinyl-based monomer and a vinyl cyanide-based monomer.