Method for quantitatively determining an analyte in a liquid sample, comprising - providing a liquid sample comprising an analyte of interest and a marker substance bound to the analyte; - preparing a mixed liquid sample by mixing the liquid sample with a hyperpolarized gas, the marker substance and the hyperpolarized gas providing a host-guest system in the mixed liquid sample, wherein the hyperpolarized gas is having a shift of nuclear magnetic resonance frequencies in case of a transition from a freely dissolved state to a host-bound state in which the hyperpolarized gas is bound to the marker substance in the mixed liquid sample; - experimentally determining the mixed liquid sample by nuclear magnetic resonance spectroscopy, comprising applying a Hyper-CEST method, which comprises: - in a first measurement, establishing a saturation transfer for the hyperpolarized gas in the freely dissolved state and the host-bound state, comprising depolarizing the hyperpolarized gas in the host-bound state by applying radio frequency (rf) irradiation in resonance with the hyperpolarized gas in the host-bound state for a time length τ, - in a second measurement, establishing a non-saturation transfer for the hyperpolarized gas in the freely dissolved state and the host-bound state; and - detecting NMR signals for the hyperpolarized gas in the freely dissolved state; - determining a concentration of the hyperpolarized gas in the mixed liquid sample for the freely dissolved state; and - quantitatively determining the analyte in the liquid sample; characterized by applying a model function S for the NMR signal as follows: S ρ τ = A ρ e − R ′ ρ τ − A ρ e − R + k on ρ τ, wherein the first term of the model function S indicates the NMR signal for off-resonant irradiation without saturation transfer and the second term of the model function indicates the NMR signal in case of the resonant irradiation with saturation transfer, and wherein ρ - concentration of the hyperpolarized gas in the freely dissolved state, k on - association rate for binding of the hyperpolarized gas to the marker substance, R - relaxation and direct saturation rate of free hyperpolarized gas in case of rf irradiation resonant with the host bound hyperpolarized gas, and R' - relaxation and direct saturation rate of free hyperpolarized gas in case of rf irradiation off-resonant with the host bound hyperpolarized gas. - providing a liquid sample comprising an analyte of interest and a marker substance bound to the analyte; - preparing a mixed liquid sample by mixing the liquid sample with a hyperpolarized gas, the marker substance and the hyperpolarized gas providing a host-guest system in the mixed liquid sample, wherein the hyperpolarized gas is having a shift of nuclear magnetic resonance frequencies in case of a transition from a freely dissolved state to a host-bound state in which the hyperpolarized gas is bound to the marker substance in the mixed liquid sample; - experimentally determining the mixed liquid sample by nuclear magnetic resonance spectroscopy, comprising applying a Hyper-CEST method, which comprises: - in a first measurement, establishing a saturation transfer for the hyperpolarized gas in the freely dissolved state and the host-bound state, comprising depolarizing the hyperpolarized gas in the host-bound state by applying radio frequency (rf) irradiation in resonance with the hyperpolarized gas in the host-bound state for a time length τ, - in a second measurement, establishing a non-saturation transfer for the hyperpolarized gas in the freely dissolved state and the host-bound state; and - detecting NMR signals for the hyperpolarized gas in the freely dissolved state; - determining a concentration of the hyperpolarized gas in the mixed liquid sample for the freely dissolved state; and - quantitatively determining the analyte in the liquid sample; - in a first measurement, establishing a saturation transfer for the hyperpolarized gas in the freely dissolved state and the host-bound state, comprising depolarizing the hyperpolarized gas in the host-bound state by applying radio frequency (rf) irradiation in resonance with the hyperpolarized gas in the host-bound state for a time length τ, - in a second measurement, establishing a non-saturation transfer for the hyperpolarized gas in the freely dissolved state and the host-bound state; and - detecting NMR signals for the hyperpolarized gas in the freely dissolved state; - determining a concentration of the hyperpolarized gas in the mixed liquid sample for the freely dissolved state; and - quantitatively determining the analyte in the liquid sample; wherein the first term of the model function S indicates the NMR signal for off-resonant irradiation without saturation transfer and the second term of the model function indicates the NMR signal in case of the resonant irradiation with saturation transfer, and wherein ρ - concentration of the hyperpolarized gas in the freely dissolved state, k on - association rate for binding of the hyperpolarized gas to the marker substance, R - relaxation and direct saturation rate of free hyperpolarized gas in case of rf irradiation resonant with the host bound hyperpolarized gas, and R' - relaxation and direct saturation rate of free hyperpolarized gas in case of rf irradiation off-resonant with the host bound hyperpolarized gas.