An engineered parental circular covalently closed synthetic plasmid DNA comprising: a synthetic DNA backbone comprising at least two linearization restriction enzymes, at least one bacterial selection system, and at least one bacterial origin of replication, and; at least one circular RNA module scaffold comprising, in the following order at least one RNA polymerase promoter, at least a first circularization sequence (circSEQ1), at least one 5' DNA spacer, at least a first restriction enzyme recognition sequence, at least one translation initiation sequence, at least a second restriction enzyme recognition sequence, at least one coding sequence, at least a third restriction enzyme recognition sequence, at least one 3' DNA spacer, and at least a second circularization sequence (circSEQ2); wherein said circularization sequences comprise homology region sequences complementary to a nucleic acid and/or a protein-based system capable of RNA ligation, said system being selected from the group consisting of an RNA ligating DNAzyme-mediated circularization system, a CRISPR/dCas9-DNA-Ligase-mediated circularization system, or a Self-Splicing Intron-Exon Mediated circularization system, and wherein the first circularization sequence (circSEQ1) of the at least two circularization sequences of the at least one circular RNA module scaffold is comprised of at least: a first restriction enzyme recognition sequence (RE1A), a first homology region (HR1B) operably linked to an exon segment (Exon I) and intron segment (Intron I) derived from permuted self-splicing and auto-catalytic group I intron-exon systems, a second restriction enzyme recognition sequence (RE1B), and a second homology region (HR1A); and, the second circularization sequence (circSEQ2) of the at least two circularization sequences of the at least one circular RNA module scaffold is comprised of at least: a first homology region (HR2B) operably linked to a first restriction enzyme recognition sequence (RE2A), an exon segment (Exon II) and intron segment (Intron II) derived from permuted self-splicing and auto-catalytic group I intron-exon systems, a second homology region (HR2A) and a second restriction enzyme recognition sequence (RE2B); the first homology region HR1A of the first circularization sequence circSEQ1 being complementary to the second homology region HR2A of the second circularization sequence circSEQ2; and, the second homology region HR1B of the first circularization sequence circSEQ1 being complementary to the first homology region HR2B of the second circularization sequence circSEQ2. a synthetic DNA backbone comprising at least two linearization restriction enzymes, at least one bacterial selection system, and at least one bacterial origin of replication, and; at least one circular RNA module scaffold comprising, in the following order at least one RNA polymerase promoter, at least a first circularization sequence (circSEQ1), at least one 5' DNA spacer, at least a first restriction enzyme recognition sequence, at least one translation initiation sequence, at least a second restriction enzyme recognition sequence, at least one coding sequence, at least a third restriction enzyme recognition sequence, at least one 3' DNA spacer, and at least a second circularization sequence (circSEQ2); wherein said circularization sequences comprise homology region sequences complementary to a nucleic acid and/or a protein-based system capable of RNA ligation, said system being selected from the group consisting of an RNA ligating DNAzyme-mediated circularization system, a CRISPR/dCas9-DNA-Ligase-mediated circularization system, or a Self-Splicing Intron-Exon Mediated circularization system, and wherein the first circularization sequence (circSEQ1) of the at least two circularization sequences of the at least one circular RNA module scaffold is comprised of at least: a first restriction enzyme recognition sequence (RE1A), a first homology region (HR1B) operably linked to an exon segment (Exon I) and intron segment (Intron I) derived from permuted self-splicing and auto-catalytic group I intron-exon systems, a second restriction enzyme recognition sequence (RE1B), and a second homology region (HR1A); and, the second circularization sequence (circSEQ2) of the at least two circularization sequences of the at least one circular RNA module scaffold is comprised of at least: a first homology region (HR2B) operably linked to a first restriction enzyme recognition sequence (RE2A), an exon segment (Exon II) and intron segment (Intron II) derived from permuted self-splicing and auto-catalytic group I intron-exon systems, a second homology region (HR2A) and a second restriction enzyme recognition sequence (RE2B); the first homology region HR1A of the first circularization sequence circSEQ1 being complementary to the second homology region HR2A of the second circularization sequence circSEQ2; and, the second homology region HR1B of the first circularization sequence circSEQ1 being complementary to the first homology region HR2B of the second circularization sequence circSEQ2.