A specimen collection container comprising: a first chamber configured to maintain a fluid, the first chamber having an open top portion, a sidewall, and a bottom portion; a second chamber having a top, a closed bottom, and a sidewall; a valve disposed between the first chamber and the second chamber and transitionable from an open position which permits fluid communication between the first chamber and the second chamber to a closed position which maintains fluid isolation between the first chamber and the second chamber; wherein, in the open position, a predetermined volume of fluid received in the first chamber may pass from the first chamber to the second chamber and, wherein when the predetermined volume of fluid passes to the second chamber, the valve transitions from the open position to the closed position such that additional fluid received within the first chamber is maintained in the first chamber in fluid isolation from the predetermined volume of fluid contained in the second chamber; an expandable material expandable to transition the valve from the open position to the closed position; a port (50, 70) having a closed position and an open position, wherein the port (50, 70) is configured to provide access to the additional fluid received within the first chamber via the port (50, 70) when the port (50, 70) is in the open position; and wherein the additional fluid is maintained in the first chamber when the valve is in the closed position and the port (50, 70) is in the closed position and the additional fluid is inaccessible from the first chamber via the port (50, 70) or via the valve when the valve is in the closed position and the port (50, 70) is in the closed position. a first chamber configured to maintain a fluid, the first chamber having an open top portion, a sidewall, and a bottom portion; a second chamber having a top, a closed bottom, and a sidewall; a valve disposed between the first chamber and the second chamber and transitionable from an open position which permits fluid communication between the first chamber and the second chamber to a closed position which maintains fluid isolation between the first chamber and the second chamber; wherein, in the open position, a predetermined volume of fluid received in the first chamber may pass from the first chamber to the second chamber and, wherein when the predetermined volume of fluid passes to the second chamber, the valve transitions from the open position to the closed position such that additional fluid received within the first chamber is maintained in the first chamber in fluid isolation from the predetermined volume of fluid contained in the second chamber; an expandable material expandable to transition the valve from the open position to the closed position; a port (50, 70) having a closed position and an open position, wherein the port (50, 70) is configured to provide access to the additional fluid received within the first chamber via the port (50, 70) when the port (50, 70) is in the open position; and wherein the additional fluid is maintained in the first chamber when the valve is in the closed position and the port (50, 70) is in the closed position and the additional fluid is inaccessible from the first chamber via the port (50, 70) or via the valve when the valve is in the closed position and the port (50, 70) is in the closed position.