Method for forming an image of a sample comprising the following steps: a) illuminating the sample with a light source; b) acquiring a plurality of images ( I i) of the sample using an image sensor, each image comprising a hologram representative of the sample, the sample being placed between the light source and the image sensor, such that: - the sample is immobile with respect to the light source between each acquisition; - no magnifying optics are placed between the sample and the image sensor; - the image sensor lies in a detection plane ( P 0), the image sensor being moved, in the detection plane, between two successive acquisitions; - each acquired image ( I i) is respectively associated with a position ( x i, y i) of the image sensor in the detection plane, each position being different from the next, each acquired image having a field of observation, each acquired image containing pixels; c) calculating a movement ( Δ i) of each acquired image with respect to a reference image ( I ref-i) in which the image sensor occupies a reference position ( X ref-i,Y ref-i); d) forming an image ( I HR), called the high-resolution image, from the acquired images and the movement ( Δ i) calculated for each thereof, the high-resolution image having a field of observation corresponding to an intersection of the fields of observation of each acquired image ( I i), the high-resolution image containing more pixels than each acquired image; the method being characterized in that step d) comprises: - obtaining a stack of subpixelated and aligned images I i, HR Δ i from each acquired image and the movements ( Δ i) determined in step c), each subpixelated and aligned image containing a number of pixels higher than the number of pixels of each acquired image ( I i); - combining the subpixelated and aligned images I i, HR Δ i in order to obtain the high-resolution image ( I HR). a) illuminating the sample with a light source; b) acquiring a plurality of images ( I i) of the sample using an image sensor, each image comprising a hologram representative of the sample, the sample being placed between the light source and the image sensor, such that: - the sample is immobile with respect to the light source between each acquisition; - no magnifying optics are placed between the sample and the image sensor; - the image sensor lies in a detection plane ( P 0), the image sensor being moved, in the detection plane, between two successive acquisitions; - each acquired image ( I i) is respectively associated with a position ( x i, y i) of the image sensor in the detection plane, each position being different from the next, each acquired image having a field of observation, each acquired image containing pixels; - the sample is immobile with respect to the light source between each acquisition; - no magnifying optics are placed between the sample and the image sensor; - the image sensor lies in a detection plane ( P 0), the image sensor being moved, in the detection plane, between two successive acquisitions; - each acquired image ( I i) is respectively associated with a position ( x i, y i) of the image sensor in the detection plane, each position being different from the next, each acquired image having a field of observation, each acquired image containing pixels; c) calculating a movement ( Δ i) of each acquired image with respect to a reference image ( I ref-i) in which the image sensor occupies a reference position ( X ref-i,Y ref-i); d) forming an image ( I HR), called the high-resolution image, from the acquired images and the movement ( Δ i) calculated for each thereof, the high-resolution image having a field of observation corresponding to an intersection of the fields of observation of each acquired image ( I i), the high-resolution image containing more pixels than each acquired image; the method being characterized in that step d) comprises: - obtaining a stack of subpixelated and aligned images I i, HR Δ i from each acquired image and the movements ( Δ i) determined in step c), each subpixelated and aligned image containing a number of pixels higher than the number of pixels of each acquired image ( I i); - combining the subpixelated and aligned images I i, HR Δ i in order to obtain the high-resolution image ( I HR). - obtaining a stack of subpixelated and aligned images I i, HR Δ i from each acquired image and the movements ( Δ i) determined in step c), each subpixelated and aligned image containing a number of pixels higher than the number of pixels of each acquired image ( I i); - combining the subpixelated and aligned images I i, HR Δ i in order to obtain the high-resolution image ( I HR).