Method for producing an antibody comprising the following steps: a) providing a plurality of cells, each expressing an antibody, b) determining the time dependent amount of said antibody bound to the respective antigen by surface plasmon resonance at different temperatures and different antibody concentrations, wherein the temperature is in the range of from 13°C to 37°C, c) calculating with the time dependent amount determined in b) based on equations to (XIII) ΔG ° = ΔH ° − T * ΔS ° ΔG ° = − R * T * lnK D lnK D = − 1 / T * ΔH ° / R) / slope − ΔS ° / R / intercept R * T * lnK D = ΔH ° T 0 − T * ΔS ° T 0 + ΔC ° p T − T 0 − T * Δ C p ° ln T / T 0 k a = k b * T / h * e − ΔG ° ‡ / R * T lnk a / T = − 1 / T * ΔH° ‡ / R / slope + ΔS ° ‡ * R + ln k b / h / intercept k a = A * e − Ea / R * T lnk a = lnA / intercept − 1 / T * Ea / R / slope k d = k b − T / h * e − ΔG ° ‡ / R * T lnk d / T = − 1 / T * ΔH ° ‡ / R / slope + ΔS ° ‡ / R + lnk B / h / intercept k d = A * e − Ea / R * T lnk d = lnA / intercept − 1 / T * Ea / R / slope at least the thermodynamic parameters (i) standard association binding entropy (ΔS°ass), (ii) standard dissociation binding entropy (AS°‡diss), (iii) standard binding entropy (ΔS°), (iv) free standard binding enthalpy (ΔG°), (v) standard dissociation free binding enthalpy (ΔG°‡diss), (vi) standard association free binding enthalpy (AG°‡ass), (vii) -TΔS°, (viii) dissociation rate constant k d, (ix) equilibrium binding constant K D, and (x) association rate constant k a, d) selecting a cell producing an antibody with at least two of the following: i) a standard association binding entropy of less than 10 J/K*mol, ii) an absolute standard dissociation binding entropy of 100 J/mol*K or more, iii) an absolute standard binding entropy of 100 J/mol*K or more, e) producing an antibody by cultivating said selected cell under conditions suitable for the expression of said antibody and recovering said antibody from the cells or/and the cultivation medium. a) providing a plurality of cells, each expressing an antibody, b) determining the time dependent amount of said antibody bound to the respective antigen by surface plasmon resonance at different temperatures and different antibody concentrations, wherein the temperature is in the range of from 13°C to 37°C, c) calculating with the time dependent amount determined in b) based on equations to (XIII) ΔG ° = ΔH ° − T * ΔS ° ΔG ° = − R * T * lnK D lnK D = − 1 / T * ΔH ° / R) / slope − ΔS ° / R / intercept R * T * lnK D = ΔH ° T 0 − T * ΔS ° T 0 + ΔC ° p T − T 0 − T * Δ C p ° ln T / T 0 k a = k b * T / h * e − ΔG ° ‡ / R * T lnk a / T = − 1 / T * ΔH° ‡ / R / slope + ΔS ° ‡ * R + ln k b / h / intercept k a = A * e − Ea / R * T lnk a = lnA / intercept − 1 / T * Ea / R / slope k d = k b − T / h * e − ΔG ° ‡ / R * T lnk d / T = − 1 / T * ΔH ° ‡ / R / slope + ΔS ° ‡ / R + lnk B / h / intercept k d = A * e − Ea / R * T lnk d = lnA / intercept − 1 / T * Ea / R / slope at least the thermodynamic parameters (i) standard association binding entropy (ΔS°ass), (ii) standard dissociation binding entropy (AS°‡diss), (iii) standard binding entropy (ΔS°), (iv) free standard binding enthalpy (ΔG°), (v) standard dissociation free binding enthalpy (ΔG°‡diss), (vi) standard association free binding enthalpy (AG°‡ass), (vii) -TΔS°, (viii) dissociation rate constant k d, (ix) equilibrium binding constant K D, and (x) association rate constant k a, (i) standard association binding entropy (ΔS°ass), (ii) standard dissociation binding entropy (AS°‡diss), (iii) standard binding entropy (ΔS°), (iv) free standard binding enthalpy (ΔG°), (v) standard dissociation free binding enthalpy (ΔG°‡diss), (vi) standard association free binding enthalpy (AG°‡ass), (vii) -TΔS°, (viii) dissociation rate constant k d, (ix) equilibrium binding constant K D, and (x) association rate constant k a, d) selecting a cell producing an antibody with at least two of the following: i) a standard association binding entropy of less than 10 J/K*mol, ii) an absolute standard dissociation binding entropy of 100 J/mol*K or more, iii) an absolute standard binding entropy of 100 J/mol*K or more, i) a standard association binding entropy of less than 10 J/K*mol, ii) an absolute standard dissociation binding entropy of 100 J/mol*K or more, iii) an absolute standard binding entropy of 100 J/mol*K or more, e) producing an antibody by cultivating said selected cell under conditions suitable for the expression of said antibody and recovering said antibody from the cells or/and the cultivation medium.