mhurhangee/ep-ind-claims huggingface dataset:
Method for the in vitro generation of genetically engineered, linear, single-stranded nucleic acid (ssNA) fragments containing two adeno-associated viral inverted terminal repeat (AAV ITR) sequences flanking a gene of interest, being essentially free of bacterial nucleic acid sequences, comprising the steps of: a) Providing a bacterial nucleic acid plasmid containing a nucleic acid fragment containing the two AAV ITR sequences and the GOI; b) Enzymatic digestion of the plasmid with a first restriction enzyme or a combination of at least two restriction enzymes and generating the double stranded nucleic acid fragment with terminal compatible ends; c) isolation of the linear double stranded nucleic acid fragment containing the nucleic acid fragment with AAV ITR sequences and GOI; d) Recirculation of the isolated linear double stranded nucleic acid fragment of step c) to obtain a double stranded circular nucleic acid fragment joint at the compatible ends formed in step b); e) Amplification of the circulated double stranded fragment of d) containing two AAV ITRs and the GOI by a nucleic acid amplification technique generating single-stranded nucleic acids comprising at least two covalently linked copies, reverse complement copies, of the template strand; f) Enzymatic digestion of the amplification product obtained in step e) with a second restriction enzyme or two different restriction enzymes and to obtain linear ssNA sequences comprising said nucleic acid fragment of the two AAV ITR sequences and the GOI; and g) Purification of said linear ssNA fragment obtained in step f) comprising the nucleic acid fragment of the two AAV ITR sequences and the GOI. a) Providing a bacterial nucleic acid plasmid containing a nucleic acid fragment containing the two AAV ITR sequences and the GOI; b) Enzymatic digestion of the plasmid with a first restriction enzyme or a combination of at least two restriction enzymes and generating the double stranded nucleic acid fragment with terminal compatible ends; c) isolation of the linear double stranded nucleic acid fragment containing the nucleic acid fragment with AAV ITR sequences and GOI; d) Recirculation of the isolated linear double stranded nucleic acid fragment of step c) to obtain a double stranded circular nucleic acid fragment joint at the compatible ends formed in step b); e) Amplification of the circulated double stranded fragment of d) containing two AAV ITRs and the GOI by a nucleic acid amplification technique generating single-stranded nucleic acids comprising at least two covalently linked copies, reverse complement copies, of the template strand; f) Enzymatic digestion of the amplification product obtained in step e) with a second restriction enzyme or two different restriction enzymes and to obtain linear ssNA sequences comprising said nucleic acid fragment of the two AAV ITR sequences and the GOI; and g) Purification of said linear ssNA fragment obtained in step f) comprising the nucleic acid fragment of the two AAV ITR sequences and the GOI.
An acceptor device for sheet objects, comprising a sensor (16, 17, 18) to derive data corresponding to a spatial array of data samples from a face of a sheet object on a path, said data being configured in a sampling frame that lies within a range of positional relationships to a reference frame; characterised by processing means (22, 23) operable to: identify an approximate mid location within the perimeter of the sheet object; define scanning lines extending outwardly from said approximate mid location and traversing locations corresponding to edges of the sheet object, scan data samples along the scanning lines to identify locations corresponding to edges of the sheet object; determine the relationship between the reference frame and the sampling frame for the sensed data based on the identified locations corresponding to edges of the sheet object, and transform pre-selected regions of the sensed data from the sampling frame so as to correspond to data in the reference frame, and to make a comparison of the transformed data with reference data corresponding to the pre-selected regions in the reference frame that define acceptability criteria for sheet objects in said reference frame, and acceptor means (24, 5) for accepting the sheet object depending on the outcome of the comparison. identify an approximate mid location within the perimeter of the sheet object; define scanning lines extending outwardly from said approximate mid location and traversing locations corresponding to edges of the sheet object, scan data samples along the scanning lines to identify locations corresponding to edges of the sheet object; determine the relationship between the reference frame and the sampling frame for the sensed data based on the identified locations corresponding to edges of the sheet object, and transform pre-selected regions of the sensed data from the sampling frame so as to correspond to data in the reference frame, and to make a comparison of the transformed data with reference data corresponding to the pre-selected regions in the reference frame that define acceptability criteria for sheet objects in said reference frame, and acceptor means (24, 5) for accepting the sheet object depending on the outcome of the comparison.
A planar flexible analyte sensor comprising: a polyester base layer having a layer of sputtered platinum formed directly on the polyester base layer; a working electrode and a reference electrode formed by patterning the layer of sputtered platinum on the base layer by laser ablation; an insulating dielectric layer over the polyester base layer and the layer of sputtered platinum, wherein the insulating dielectric layer leaves a portion of the working electrode exposed and a portion of the reference electrode exposed; silver/silver chloride ink on the exposed portion of the reference electrode; a glucose oxidase layer over the exposed portion of the working electrode; and a glucose limiting membrane over the glucose oxidase layer. a polyester base layer having a layer of sputtered platinum formed directly on the polyester base layer; a working electrode and a reference electrode formed by patterning the layer of sputtered platinum on the base layer by laser ablation; an insulating dielectric layer over the polyester base layer and the layer of sputtered platinum, wherein the insulating dielectric layer leaves a portion of the working electrode exposed and a portion of the reference electrode exposed; silver/silver chloride ink on the exposed portion of the reference electrode; a glucose oxidase layer over the exposed portion of the working electrode; and a glucose limiting membrane over the glucose oxidase layer.
Vehicle seat comprising at least: - a seat support structure which is adjustable between an initial upright position and at least a fully inclined position, - a track assembly configured to adjust the seat support structure in a longitudinal direction, and - a slide assembly configured to adjust the seat support structure in a substantially vertical direction, wherein the seat support structure comprises at least - a seat pan (2.1) which is mounted to the track assembly, and - a backrest (2.2) which is mounted to the seat pan (2.1) and which is coupled to the slide assembly, wherein during a longitudinal adjustment of the seat support structure between a first horizontal position and a second horizontal position, at least the backrest (2.2) is moved in the vertical direction between a first vertical position and a second vertical position, and wherein the backrest (2.2) is attached to the slide assembly through a free pivot such that the backrest (2.2) pivots and inclines as the seat support structure travels forward and partially lower, thereby a rear region (2.1.2) of the seat pan (2.1) drops and a front region (2.1.1) of the seat pan (2.1) raises while the backrest (2.2) is reclined. - a seat support structure which is adjustable between an initial upright position and at least a fully inclined position, - a track assembly configured to adjust the seat support structure in a longitudinal direction, and - a slide assembly configured to adjust the seat support structure in a substantially vertical direction, wherein the seat support structure comprises at least - a seat pan (2.1) which is mounted to the track assembly, and - a backrest (2.2) which is mounted to the seat pan (2.1) and which is coupled to the slide assembly, wherein during a longitudinal adjustment of the seat support structure between a first horizontal position and a second horizontal position, at least the backrest (2.2) is moved in the vertical direction between a first vertical position and a second vertical position, and wherein the backrest (2.2) is attached to the slide assembly through a free pivot such that the backrest (2.2) pivots and inclines as the seat support structure travels forward and partially lower, thereby a rear region (2.1.2) of the seat pan (2.1) drops and a front region (2.1.1) of the seat pan (2.1) raises while the backrest (2.2) is reclined.
Nanovesicles, wherein the nanovesicles are derived from membranes of cells, intracellular components of which have been removed by treating the cells with an alkaline solution of pH 9 to 14, and a size of the nanovesicles is smaller than a size of the cells.
A surgical sealant comprising: a first agent containing a hydrophobically-modified gelatin derived from a cold-water fish, and a second agent containing a water-soluble molecule for crosslinking, wherein the water-soluble molecule for crosslinking is at least one kind selected from the group consisting of polyethylene glycol disuccinimidyl succinate and pentaerythritol-poly (ethylene glycol) ether tetrasuccinimidyl glutalate, the hydrophobically-modified gelatin derived from a cold-water fish is a gelatin in which at least a part of amino groups of side chains of a gelatin derived from a cold-water fish has been substituted by hydrophobic groups, the hydrophobic groups are linear chain aliphatic groups each having 8 to 18 carbon atoms, with a substitution rate (number of moles of hydrophobic groups / (total number of moles of hydrophobic groups and reactive amino groups in gelatin) × 100) of 3 to 20 mol%, and the first agent is used in the form of an aqueous solution having a pH of 9 to 11, and the second agent is used in the form of an aqueous solution having a pH of 4 to 6. a first agent containing a hydrophobically-modified gelatin derived from a cold-water fish, and a second agent containing a water-soluble molecule for crosslinking, wherein the water-soluble molecule for crosslinking is at least one kind selected from the group consisting of polyethylene glycol disuccinimidyl succinate and pentaerythritol-poly (ethylene glycol) ether tetrasuccinimidyl glutalate, the hydrophobically-modified gelatin derived from a cold-water fish is a gelatin in which at least a part of amino groups of side chains of a gelatin derived from a cold-water fish has been substituted by hydrophobic groups, the hydrophobic groups are linear chain aliphatic groups each having 8 to 18 carbon atoms, with a substitution rate (number of moles of hydrophobic groups / (total number of moles of hydrophobic groups and reactive amino groups in gelatin) × 100) of 3 to 20 mol%, and
A drum for a washing machine comprising: a center comprising a cylindrical body; a back coupled to a back portion of the center; a plurality of holes formed at the back; a front coupled to a front portion of the center and having an opening; a recess formed at the back and configured to accommodate a spider connected to a driving shaft; wherein the recess has the same shape as the spider such that the spider is tightly coupled to the back and wherein the recess accommodates the arms (35, 36, 37) of the spider, characterised in that the drum further comprises at least one bent portion (52, 53, 54) formed within the portions of the recess accommodating the arms (35, 36, 37) and projecting backwardly. a center comprising a cylindrical body; a back coupled to a back portion of the center; a plurality of holes formed at the back; a front coupled to a front portion of the center and having an opening; a recess formed at the back and configured to accommodate a spider connected to a driving shaft; wherein the recess has the same shape as the spider such that the spider is tightly coupled to the back and wherein the recess accommodates the arms (35, 36, 37) of the spider, characterised in that the drum further comprises at least one bent portion (52, 53, 54) formed within the portions of the recess accommodating the arms (35, 36, 37) and projecting backwardly.
A color hologram display comprising a single-layer recorded photosensitive material, wherein a color plane pattern (1'a) and a color three-dimensional subject image (○') are recorded as reflection and volume type holograms in the photosensitive material in such a way that said color plane pattern and said color three-dimensional subject image (○") are reconstructed while spatially superposed one upon another, characterized in that: a plane shadow pattern having the shape of said color plane pattern (1'a) is recorded as a reflection and volume type hologram in the photosensitive material in such a way that said plane shadow pattern is reconstructed on a surface different from a surface of said reconstructed color plane pattern, said color plane pattern is reconstructed in monochrome under illumination with white light, and said plane shadow pattern is reconstructed in a complementary color to the monochrome color of said color plane pattern under white light illumination. a plane shadow pattern having the shape of said color plane pattern (1'a) is recorded as a reflection and volume type hologram in the photosensitive material in such a way that said plane shadow pattern is reconstructed on a surface different from a surface of said reconstructed color plane pattern, said color plane pattern is reconstructed in monochrome under illumination with white light, and said plane shadow pattern is reconstructed in a complementary color to the monochrome color of said color plane pattern under white light illumination.
A needle and needle shield assembly wherein said needle has a tip, comprising: a retention washer integrally formed with a flexural hinge which is in turn integrally formed with an actuating arm selectively engaged to the needle, wherein the retention washer, the flexural hinge and the actuating arm are positioned in a shield body having a sidewall, a proximal end wall and a distal end, and a feature on the needle proximate to the tip that is sized to prevent passage of the needle through an opening in the proximal end wall of the needle shield, characterized in that the flexural hinge is integrally formed with a canting member movable between an aligned condition and an off-alignment condition with the needle, wherein, in the aligned condition, the canting member is maintained in alignment with the needle by the cooperation of the force exerted by the flexural hinge and the restraint exerted by the actuating arm, said canting member integrally formed with the actuating arm selectively engaged to the needle, and being maintained by the actuation arm in the aligned condition until the needle tip is proximally withdrawn, wherein, as the needle tip is withdrawn, the actuating arm comes out of engagement with the needle tip and is therefore free to move within the shield body, such that the canting member succumbs to the bias exerted by the flexural hinge, such that it bindingly engages to the exterior of the needle. a retention washer integrally formed with a flexural hinge which is in turn integrally formed with an actuating arm selectively engaged to the needle, wherein the retention washer, the flexural hinge and the actuating arm are positioned in a shield body having a sidewall, a proximal end wall and a distal end, and a feature on the needle proximate to the tip that is sized to prevent passage of the needle through an opening in the proximal end wall of the needle shield, characterized in that the flexural hinge is integrally formed with a canting member movable between an aligned condition and an off-alignment condition with the needle, wherein, in the aligned condition, the canting member is maintained in alignment with the needle by the cooperation of the force exerted by the flexural hinge and the restraint exerted by the actuating arm, said canting member integrally formed with the actuating arm selectively engaged to the needle, and being maintained by the actuation arm in the aligned condition until the needle tip is proximally withdrawn, wherein, as the needle tip is withdrawn, the actuating arm comes out of engagement with the needle tip and is therefore free to move within the shield body, such that the canting member succumbs to the bias exerted by the flexural hinge, such that it bindingly engages to the exterior of the needle.
Grinding method for the simultaneous production of a workpiece end face, as a first functional surface, and of a chamfer, as a second functional surface contiguous to the first functional surface, on a workpiece by means of a rotationally symmetrical grinding tool, comprising the steps: - rotation of the workpiece about a workpiece rotation axis and rotation of the tool about a tool rotation axis, - setting of the tool rotation axis and/or of the workpiece rotation axis in relation to one another at a set angle (α), and - movement of the workpiece and/or of the tool towards one another, - the first functional surface of the workpiece being machined by means of a circular machining edge or an annular tool end face of a basic body of the tool, and - the second functional surface of the workpiece being machined by means of a conical extension on the tool end face. - rotation of the workpiece about a workpiece rotation axis and rotation of the tool about a tool rotation axis, - setting of the tool rotation axis and/or of the workpiece rotation axis in relation to one another at a set angle (α), and - movement of the workpiece and/or of the tool towards one another, - the first functional surface of the workpiece being machined by means of a circular machining edge or an annular tool end face of a basic body of the tool, and - the second functional surface of the workpiece being machined by means of a conical extension on the tool end face.
Cooking device having a cooking cavity and at least two slide-in grids which - can be arranged on a respective side wall (3, 4) of the cooking cavity, - are designed to assume in the cooking cavity a folded-up position substantially parallel to the respective side wall (3, 4), and a folded-down, substantially horizontal position and - in their folded-down position form a grating characterised in that - the slide-in grids are fixed to a respective side wall (3, 4) of the cooking cavity by means of at least one joint, about which they can pivot. - can be arranged on a respective side wall (3, 4) of the cooking cavity, - are designed to assume in the cooking cavity a folded-up position substantially parallel to the respective side wall (3, 4), and a folded-down, substantially horizontal position and - in their folded-down position form a grating characterised in that - the slide-in grids are fixed to a respective side wall (3, 4) of the cooking cavity by means of at least one joint, about which they can pivot.
A rechargeable battery (101, 102, 103) comprising: an electrode assembly comprising a first electrode and a second electrode; a case containing the electrode assembly; a cap plate covering an opening of the case; a first terminal protruding outside of the case and electrically coupled to the first electrode; a second terminal protruding outside of the case and electrically coupled to the second electrode; a current collector (41, 60, 80) comprising a first fuse unit (41e, 64, 84) and a second fuse unit (41f, 65, 85), and electrically coupled to the first electrode, wherein the first fuse unit (41e, 64, 84) and the second fuse unit (41f, 65, 85) each have a cross-sectional area smaller than a cross-sectional area of an adjacent region and are spaced from each other; and a connector (46, 70, 90) coupled to the current collector (41, 60, 80) between the first fuse unit (41e, 64, 84) and the second fuse unit (41f, 65, 85), and adapted to electrically couple the cap plate and the current collector (41, 60, 80), characterized in that the connector (46, 70, 90) has a lower resistance than the first terminal. an electrode assembly comprising a first electrode and a second electrode; a case containing the electrode assembly; a cap plate covering an opening of the case; a first terminal protruding outside of the case and electrically coupled to the first electrode; a second terminal protruding outside of the case and electrically coupled to the second electrode; a current collector (41, 60, 80) comprising a first fuse unit (41e, 64, 84) and a second fuse unit (41f, 65, 85), and electrically coupled to the first electrode, wherein the first fuse unit (41e, 64, 84) and the second fuse unit (41f, 65, 85) each have a cross-sectional area smaller than a cross-sectional area of an adjacent region and are spaced from each other; and a connector (46, 70, 90) coupled to the current collector (41, 60, 80) between the first fuse unit (41e, 64, 84) and the second fuse unit (41f, 65, 85), and adapted to electrically couple the cap plate and the current collector (41, 60, 80), characterized in that the connector (46, 70, 90) has a lower resistance than the first terminal.
A hydrogen-peroxide neutralizing medium comprising at least 0.3 U/ml of a secreted fungal catalase, wherein said fungal catalase is comprised in the medium.
A pump assembly with a terminal box which comprises at least one first and a second housing part, which are pivotably connected to one another via at least one joint, characterised in that the joint with respect to the greatest extension (b) of the terminal box in the direction parallel to the pivot axis of the joint, is arranged in a central region of the terminal box in the direction of its pivot axis has an extension (a) which corresponds to less than half of the greatest extension (b) of the terminal box in the direction parallel to this pivot axis.
Packaging made in plastic material for food or food products ready for consumption, comprising a first container of larger size, for a main food component, said first container comprising a first bottom and first closure lid having a top part surrounded by side walls (17, 18), and at least one second container, of smaller size, able to separately contain a supplementary food component (5, 6), said first container having at least one cavity open to the outside, formed on the top part of said first closure lid and conformed to constitute a housing compartment for said at least one second container by at least partial insertion of said at least one second container in said at least one cavity of said first container, said closure lid of the first container having at least one groove formed as one-piece with said top part of the lid, said packaging characterised in that each groove is formed in correspondence with said at least one cavity and each groove being in addition open or faced towards the corresponding cavity involving also a part of the side walls (17, 18) of the lid of the first container so as to be also open to the outside of the first container.