A method for quantitating at least one nucleic acid target, the method comprising: amplifying a plurality of target sequences by PCR in the presence of a detectable reporter construct; acquiring signal information for each target sequence associated with the detectable reporter construct; identifying an exponential region associated with increases in the signal intensity for each target sequence amplification wherein each identified exponential region comprises an associated upper and lower bound; normalizing the signal information for each target sequence amplification using an identified baseline component for the signal information associated with each target sequence amplification; determining an exponential region threshold based upon a comparison of a plurality of exponential regions by utilizing the upper and lower bounds of the plurality of exponential regions, wherein each exponential region of the plurality of exponential regions is associated with a target sequence amplification of the plurality of target sequence amplifications, wherein each exponential region includes an upper bound and lower bound, and wherein determining the exponential region threshold comprises: identifying a minimal upper bound from the upper bounds of the plurality of exponential regions, identifying a maximal lower bound from the lower bounds of the exponential regions, if there is an intersection region between a minimal upper bound of the plurality of upper bounds and a maximal lower bound of the plurality of lower bounds, determining the exponential region threshold upon identification of the intersection region, and if there is no intersection region, discarding a target sequence amplification from the signal information and re-identifying the exponential region upper bound and the exponential region lower bound until an intersection is identified; identifying, based on the exponential region threshold, a threshold cycle for each target sequence amplification using an associated polynomial root; and quantifying each target sequence based on the threshold cycle. amplifying a plurality of target sequences by PCR in the presence of a detectable reporter construct; acquiring signal information for each target sequence associated with the detectable reporter construct; identifying an exponential region associated with increases in the signal intensity for each target sequence amplification wherein each identified exponential region comprises an associated upper and lower bound; normalizing the signal information for each target sequence amplification using an identified baseline component for the signal information associated with each target sequence amplification; determining an exponential region threshold based upon a comparison of a plurality of exponential regions by utilizing the upper and lower bounds of the plurality of exponential regions, wherein each exponential region of the plurality of exponential regions is associated with a target sequence amplification of the plurality of target sequence amplifications, wherein each exponential region includes an upper bound and lower bound, and wherein determining the exponential region threshold comprises: identifying a minimal upper bound from the upper bounds of the plurality of exponential regions, identifying a maximal lower bound from the lower bounds of the exponential regions, if there is an intersection region between a minimal upper bound of the plurality of upper bounds and a maximal lower bound of the plurality of lower bounds, determining the exponential region threshold upon identification of the intersection region, and if there is no intersection region, discarding a target sequence amplification from the signal information and re-identifying the exponential region upper bound and the exponential region lower bound until an intersection is identified; identifying, based on the exponential region threshold, a threshold cycle for each target sequence amplification using an associated polynomial root; and quantifying each target sequence based on the threshold cycle.