Device for preparing a calibrated volume of blood plasma from a blood sample, characterized in that it comprises: - a card comprising a fluidic network, - a plurality of modules arranged on the card, comprising a fluidic connection module, a module for separation of the blood plasma contained in said blood sample and a module for obtaining a calibrated volume of blood plasma obtained after separation, - said fluidic connection module, comprising: ∘ at least one fluidic connection member which is adapted to connect a device for taking a blood sample and which comprises a first fluidic inlet of said fluidic network via which the blood sample taken is introduced, ∘ a first fluidic outlet of said fluidic network connected to said first fluidic inlet, ∘ means for releasing the blood sample via said first fluidic inlet, - said module for separation of the blood plasma contained in said blood sample, comprising: ∘ a chamber formed in the card, ∘ a separation membrane separating said chamber into a first space and a second space, ∘ at least a second fluidic inlet of said fluidic network connected to said first fluidic outlet of the connection module and opening out into the first space of the chamber, ∘ a second fluidic outlet of said fluidic network opening out into said second space of the chamber, - said module for obtaining a calibrated volume of blood plasma obtained after separation, comprising: ∘ at least one fluidic pumping assembly comprising at least one pump, ∘ a first fluidic link connecting said second fluidic outlet via a first fluidic valve of said fluidic network to the pumping assembly, ∘ a first collection reservoir provided with a first vent and connected to the pumping assembly via a second fluidic link commanded by a second fluidic valve of the network, ∘ a second collection reservoir provided with a second vent and connected to the pumping assembly via a third fluidic link commanded by a third fluidic valve of the network, ∘ said at least one pump comprising a cavity that has a volume that corresponds to a unit volume of the calibrated volume to be obtained in the second collection reservoir. - a card comprising a fluidic network, - a plurality of modules arranged on the card, comprising a fluidic connection module, a module for separation of the blood plasma contained in said blood sample and a module for obtaining a calibrated volume of blood plasma obtained after separation, - said fluidic connection module, comprising: ∘ at least one fluidic connection member which is adapted to connect a device for taking a blood sample and which comprises a first fluidic inlet of said fluidic network via which the blood sample taken is introduced, ∘ a first fluidic outlet of said fluidic network connected to said first fluidic inlet, ∘ means for releasing the blood sample via said first fluidic inlet, ∘ at least one fluidic connection member which is adapted to connect a device for taking a blood sample and which comprises a first fluidic inlet of said fluidic network via which the blood sample taken is introduced, ∘ a first fluidic outlet of said fluidic network connected to said first fluidic inlet, ∘ means for releasing the blood sample via said first fluidic inlet, - said module for separation of the blood plasma contained in said blood sample, comprising: ∘ a chamber formed in the card, ∘ a separation membrane separating said chamber into a first space and a second space, ∘ at least a second fluidic inlet of said fluidic network connected to said first fluidic outlet of the connection module and opening out into the first space of the chamber, ∘ a second fluidic outlet of said fluidic network opening out into said second space of the chamber, ∘ a chamber formed in the card, ∘ a separation membrane separating said chamber into a first space and a second space, ∘ at least a second fluidic inlet of said fluidic network connected to said first fluidic outlet of the connection module and opening out into the first space of the chamber, ∘ a second fluidic outlet of said fluidic network opening out into said second space of the chamber, - said module for obtaining a calibrated volume of blood plasma obtained after separation, comprising: ∘ at least one fluidic pumping assembly comprising at least one pump, ∘ a first fluidic link connecting said second fluidic outlet via a first fluidic valve of said fluidic network to the pumping assembly, ∘ a first collection reservoir provided with a first vent and connected to the pumping assembly via a second fluidic link commanded by a second fluidic valve of the network, ∘ a second collection reservoir provided with a second vent and connected to the pumping assembly via a third fluidic link commanded by a third fluidic valve of the network, ∘ said at least one pump comprising a cavity that has a volume that corresponds to a unit volume of the calibrated volume to be obtained in the second collection reservoir. ∘ at least one fluidic pumping assembly comprising at least one pump, ∘ a first fluidic link connecting said second fluidic outlet via a first fluidic valve of said fluidic network to the pumping assembly, ∘ a first collection reservoir provided with a first vent and connected to the pumping assembly via a second fluidic link commanded by a second fluidic valve of the network, ∘ a second collection reservoir provided with a second vent and connected to the pumping assembly via a third fluidic link commanded by a third fluidic valve of the network, ∘ said at least one pump comprising a cavity that has a volume that corresponds to a unit volume of the calibrated volume to be obtained in the second collection reservoir.