A system, comprising: an introducer sheath including a retention member configured to anchor the introducer sheath in a natural body lumen having a wall; an elongate member (40; 70; 80; 1110) extending along a longitudinal axis through a working channel of the introducer sheath; and a manipulating portion (42; 72; 82; 1100; 1200; 1300) coupled to a distal end of the elongate member (40; 70; 80; 1110) including at least one selectively actuatable member (46; 76; 86; 1120, 1130; 1220, 1230; 1320, 1330) configured to transition between an undeployed configuration and a deployed configuration, wherein in the undeployed configuration the at least one actuatable member (46; 76; 86; 1120, 1130; 1220, 1230; 1320, 1330) extends substantially parallel to the longitudinal axis, and wherein in at least one position in the deployed configuration, the at least one actuatable member (46; 76; 86; 1120, 1130; 1220, 1230; 1320, 1330) extends radially outwardly from the longitudinal axis; and wherein the at least one actuatable member is a first member (1120; 1220; 1320), the system further comprising: a second selectively actuatable member (1130; 1230; 1330), wherein the second actuatable member is configured to transition between an undeployed configuration and a deployed configuration, wherein in the undeployed configuration the second actuatable member extends substantially parallel to the longitudinal axis, and wherein in at least one position in the deployed configuration, the second actuatable member extends radially outwardly from the longitudinal axis; characterized in that each of the first and second actuatable members (1120, 1130) are configured to rotate approximately 180° relative to the longitudinal axis so as to occlude the lumen in a closed configuration; and each of the first and second actuatable members (1120, 1130) includes a geometrical feature (1140, 1150, 1160) to maintain the first and second actuatable members (1120, 1130) in the closed configuration. an introducer sheath including a retention member configured to anchor the introducer sheath in a natural body lumen having a wall; an elongate member (40; 70; 80; 1110) extending along a longitudinal axis through a working channel of the introducer sheath; and a manipulating portion (42; 72; 82; 1100; 1200; 1300) coupled to a distal end of the elongate member (40; 70; 80; 1110) including at least one selectively actuatable member (46; 76; 86; 1120, 1130; 1220, 1230; 1320, 1330) configured to transition between an undeployed configuration and a deployed configuration, wherein in the undeployed configuration the at least one actuatable member (46; 76; 86; 1120, 1130; 1220, 1230; 1320, 1330) extends substantially parallel to the longitudinal axis, and wherein in at least one position in the deployed configuration, the at least one actuatable member (46; 76; 86; 1120, 1130; 1220, 1230; 1320, 1330) extends radially outwardly from the longitudinal axis; and wherein the at least one actuatable member is a first member (1120; 1220; 1320), the system further comprising: a second selectively actuatable member (1130; 1230; 1330), wherein the second actuatable member is configured to transition between an undeployed configuration and a deployed configuration, wherein in the undeployed configuration the second actuatable member extends substantially parallel to the longitudinal axis, and wherein in at least one position in the deployed configuration, the second actuatable member extends radially outwardly from the longitudinal axis; characterized in that each of the first and second actuatable members (1120, 1130) are configured to rotate approximately 180° relative to the longitudinal axis so as to occlude the lumen in a closed configuration; and each of the first and second actuatable members (1120, 1130) includes a geometrical feature (1140, 1150, 1160) to maintain the first and second actuatable members (1120, 1130) in the closed configuration. a second selectively actuatable member (1130; 1230; 1330), wherein the second actuatable member is configured to transition between an undeployed configuration and a deployed configuration, wherein in the undeployed configuration the second actuatable member extends substantially parallel to the longitudinal axis, and wherein in at least one position in the deployed configuration, the second actuatable member extends radially outwardly from the longitudinal axis; characterized in that each of the first and second actuatable members (1120, 1130) are configured to rotate approximately 180° relative to the longitudinal axis so as to occlude the lumen in a closed configuration; and each of the first and second actuatable members (1120, 1130) includes a geometrical feature (1140, 1150, 1160) to maintain the first and second actuatable members (1120, 1130) in the closed configuration.