A fluorescence imaging video system configured for acquiring color and multichannel fluorescence/reflectance images, including: an insertion portion of an endoscope; a single multi-mode light source adapted to produce both multi-wavelength excitation light for fluorescence imaging and illumination light having red, green and blue components, a plurality of light source filters (76A, 76B) positionable between the light source and an illumination optical transmission system, adapted to transmit substantially all the multi-wavelength excitation light intensity and adapted to transmit selectively a predetermined portion of one or more of the red, green and blue component intensity; the optical transmission system being adapted to direct the filtered light to a tissue sample and an imaging optical transmission system adapted to collect reflected light and multi-wavelength fluorescence light produced by the tissue; a camera, located in said insertion portion, and adapted to receive the light collected by the optical transmission system, the camera including: a first color image sensor, and a second color image sensor having spectral filter, wherein each of the light source filters (76A, 76B) are adapted to be positioned stationarily during an imaging mode, the camera further comprises a beam splitter that is adapted to direct reflectance images onto the first color image sensor and multi-wavelength and fluorescence light onto the second color image sensor, the first color image sensor has a first spectral filter positioned in front of the color image sensor said filter being adapted to block selectively the multi-wavelength excitation light and transmit reflectance light at wavelengths other than the multi-wavelength excitation light, and the spectral filter of the second color image sensor is a second spectral filter, positioned in front of the color image sensor said filter being adapted to block selectively the multi-wavelength excitation light and transmit multi-wavelength fluorescence light at wavelengths other than the multi-wavelength excitation light. an insertion portion of an endoscope; a single multi-mode light source adapted to produce both multi-wavelength excitation light for fluorescence imaging and illumination light having red, green and blue components, a plurality of light source filters (76A, 76B) positionable between the light source and an illumination optical transmission system, adapted to transmit substantially all the multi-wavelength excitation light intensity and adapted to transmit selectively a predetermined portion of one or more of the red, green and blue component intensity; the optical transmission system being adapted to direct the filtered light to a tissue sample and an imaging optical transmission system adapted to collect reflected light and multi-wavelength fluorescence light produced by the tissue; a camera, located in said insertion portion, and adapted to receive the light collected by the optical transmission system, the camera including: a first color image sensor, and a second color image sensor having spectral filter, wherein each of the light source filters (76A, 76B) are adapted to be positioned stationarily during an imaging mode, a first color image sensor, and a second color image sensor having spectral filter, wherein each of the light source filters (76A, 76B) are adapted to be positioned stationarily during an imaging mode, the camera further comprises a beam splitter that is adapted to direct reflectance images onto the first color image sensor and multi-wavelength and fluorescence light onto the second color image sensor, the first color image sensor has a first spectral filter positioned in front of the color image sensor said filter being adapted to block selectively the multi-wavelength excitation light and transmit reflectance light at wavelengths other than the multi-wavelength excitation light, and the spectral filter of the second color image sensor is a second spectral filter, positioned in front of the color image sensor said filter being adapted to block selectively the multi-wavelength excitation light and transmit multi-wavelength fluorescence light at wavelengths other than the multi-wavelength excitation light.