A method of conducting a binding assay comprising (a) contacting (i) a sample comprising a target analyte with (ii) a particle linked to a first binding reagent that binds said target analyte to form a complex comprising said target analyte bound to said first binding reagent, wherein said first binding reagent is linked to a first targeting agent and said particle is linked to a second targeting agent, and said first binding reagent and said particle are linked via a binding reaction between said first and second targeting agents, and wherein the first and second targeting agents comprise an oligonucleotide and oligonucleotide complement, respectively; (b) collecting said complex in a first assay volume; (c) separating unbound components of said sample from said complex; (d) releasing said complex in a second assay volume by disassociating said binding interaction between said first and second targeting agents, wherein said second assay volume is smaller than said first assay volume, thereby releasing said first binding reagent from said particle to yield a concentrated sample of analyte bound to said first binding reagent in said complex; (e) contacting said concentrated sample with a solid phase linked to a second binding reagent that binds to said complex or a component thereof to form a solid phase-bound complex, wherein the solid phase comprises an electrode; (f) applying a voltage waveform to said electrode; and (g) measuring an electrochemiluminescent signal emitted by an ECL label linked to a component of said assay and correlating said signal with an amount of analyte in said sample. (a) contacting (i) a sample comprising a target analyte with (ii) a particle linked to a first binding reagent that binds said target analyte to form a complex comprising said target analyte bound to said first binding reagent, wherein said first binding reagent is linked to a first targeting agent and said particle is linked to a second targeting agent, and said first binding reagent and said particle are linked via a binding reaction between said first and second targeting agents, and wherein the first and second targeting agents comprise an oligonucleotide and oligonucleotide complement, respectively; (i) a sample comprising a target analyte with (ii) a particle linked to a first binding reagent that binds said target analyte to form a complex comprising said target analyte bound to said first binding reagent, wherein said first binding reagent is linked to a first targeting agent and said particle is linked to a second targeting agent, and said first binding reagent and said particle are linked via a binding reaction between said first and second targeting agents, and wherein the first and second targeting agents comprise an oligonucleotide and oligonucleotide complement, respectively; (b) collecting said complex in a first assay volume; (c) separating unbound components of said sample from said complex; (d) releasing said complex in a second assay volume by disassociating said binding interaction between said first and second targeting agents, wherein said second assay volume is smaller than said first assay volume, thereby releasing said first binding reagent from said particle to yield a concentrated sample of analyte bound to said first binding reagent in said complex; (e) contacting said concentrated sample with a solid phase linked to a second binding reagent that binds to said complex or a component thereof to form a solid phase-bound complex, wherein the solid phase comprises an electrode; (f) applying a voltage waveform to said electrode; and (g) measuring an electrochemiluminescent signal emitted by an ECL label linked to a component of said assay and correlating said signal with an amount of analyte in said sample.