A method for producing D-serine, wherein D-serine is synthesized by allowing glycine to react with formaldehyde in the presence of: (a) a microorganism having activity of synthesizing D-serine from glycine and formaldehyde, wherein the microorganism is a transformant obtained by transforming a host cell using a recombinant DNA constructed by integrating, into a vector, DNA encoding D-threonine aldolase from Xanthomonas oryzae; or (b) a treated product of the microorganism of (a) which is obtained by allowing the microorganism to be subjected to mechanical disruption, ultrasonication, freezing and thawing treatment, drying treatment, pressurization or depressurization treatment, osmotic pressure treatment, autodigestion, surfactant treatment, or enzyme treatment for the purpose of cell digestion, and which comprises an enzyme having activity of synthesizing D-serine from glycine and formaldehyde; or (c) a treated product of the microorganism of (a) which is obtained via a treatment of (b) as an immobilized fraction of the microorganism, and which comprises an enzyme having activity of synthesizing D-serine from glycine and formaldehyde; or (d) a treated product of the microorganism of (a) which is obtained by immobilizing the microorganism, and which has activity of synthesizing D-serine from glycine and formaldehyde, wherein formation of L-serine as a byproduct in the reaction solution is restrained in a manner such that L-serine accounts for 1.5 mol% or less relative to D-serine during the reaction by one or more of the following (i) and (ii): (i) allowing the microorganism or treated product thereof to be subjected to an organic solvent treatment before D-serine production; and (ii) controlling formaldehyde concentration in a reaction solution a) to 2M or less in a case in which the organic solvent treatment is carried out in accordance with (i) above and b) to from 150 mM to 2M inclusive in a case in which an organic solvent treatment is not carried out. (a) a microorganism having activity of synthesizing D-serine from glycine and formaldehyde, wherein the microorganism is a transformant obtained by transforming a host cell using a recombinant DNA constructed by integrating, into a vector, DNA encoding D-threonine aldolase from Xanthomonas oryzae; or (b) a treated product of the microorganism of (a) which is obtained by allowing the microorganism to be subjected to mechanical disruption, ultrasonication, freezing and thawing treatment, drying treatment, pressurization or depressurization treatment, osmotic pressure treatment, autodigestion, surfactant treatment, or enzyme treatment for the purpose of cell digestion, and which comprises an enzyme having activity of synthesizing D-serine from glycine and formaldehyde; or (c) a treated product of the microorganism of (a) which is obtained via a treatment of (b) as an immobilized fraction of the microorganism, and which comprises an enzyme having activity of synthesizing D-serine from glycine and formaldehyde; or (d) a treated product of the microorganism of (a) which is obtained by immobilizing the microorganism, and which has activity of synthesizing D-serine from glycine and formaldehyde, (i) allowing the microorganism or treated product thereof to be subjected to an organic solvent treatment before D-serine production; and (ii) controlling formaldehyde concentration in a reaction solution a) to 2M or less in a case in which the organic solvent treatment is carried out in accordance with (i) above and b) to from 150 mM to 2M inclusive in a case in which an organic solvent treatment is not carried out.