A detection device for detecting analytes in a liquid specimen, comprising: a specimen chamber (61, 601) for collecting or storing the liquid specimen; a detecting chamber (40, 400) containing a detecting element; a liquid transferring chamber; and a through hole located on the liquid transferring chamber for passing the liquid specimen between the specimen chamber (61, 601) and the detecting chamber (40, 400), the through hole having a first and a second status, characterized in that when the through hole is in the first status, it is self-closed or self-sealed and the liquid transferring chamber is in liquid communication with the specimen chamber (61, 601) and when the through hole is in the second status it is open and the liquid transferring chamber is in liquid communication with the detecting chamber (40, 400) via the open through hole, wherein the through hole is on a flexible element (13, 30) in the liquid transferring chamber, wherein the flexible element (13, 30) comprises a first and a second status; wherein when the flexible element (13, 30) is in the first status, the through hole is in the first status, the specimen chamber (61, 601) is in liquid communication with the liquid transferring chamber optionally via a channel, whereas the detecting chamber (40, 400) is not in liquid communication with the liquid transferring chamber; and when the flexible element (13, 30) is in the second status, the through hole is in the second status, the detecting chamber (40, 400) is in liquid communication with the liquid transferring chamber, whereas the specimen chamber (61, 601) is not in liquid communication with the liquid transferring chamber. a specimen chamber (61, 601) for collecting or storing the liquid specimen; a detecting chamber (40, 400) containing a detecting element; a liquid transferring chamber; and a through hole located on the liquid transferring chamber for passing the liquid specimen between the specimen chamber (61, 601) and the detecting chamber (40, 400), the through hole having a first and a second status, characterized in that when the through hole is in the first status, it is self-closed or self-sealed and the liquid transferring chamber is in liquid communication with the specimen chamber (61, 601) and when the through hole is in the second status it is open and the liquid transferring chamber is in liquid communication with the detecting chamber (40, 400) via the open through hole, wherein the through hole is on a flexible element (13, 30) in the liquid transferring chamber, wherein the flexible element (13, 30) comprises a first and a second status; wherein when the flexible element (13, 30) is in the first status, the through hole is in the first status, the specimen chamber (61, 601) is in liquid communication with the liquid transferring chamber optionally via a channel, whereas the detecting chamber (40, 400) is not in liquid communication with the liquid transferring chamber; and when the flexible element (13, 30) is in the second status, the through hole is in the second status, the detecting chamber (40, 400) is in liquid communication with the liquid transferring chamber, whereas the specimen chamber (61, 601) is not in liquid communication with the liquid transferring chamber.