A computer-implemented estimation method for estimating an amount of a quantified material injected into a container by a liquid droplet ejection apparatus, the amount of the material having variation according to a probability distribution, and a probability that the amount of the material is within a range of a lower limit tolerance to an upper limit tolerance on the probability distribution being greater than or equal to a target probability, the estimating method comprising: calculating the probability distribution, wherein the calculating the probability distribution includes computing the probability distribution using a performance property of the liquid droplet ejection apparatus configured to inject the material into the container, a first step of setting the target probability; a second step of computing the representative value of the amount of material on the probability distribution; a third step of setting an interval of a lower limit to an upper limit including the representative value on the probability distribution; a fourth step of setting a variable width of the interval; a fifth step of increasing a size of the interval by the variable width from the representative value toward the lower limit, and computing a first probability that the amount of the material is within the interval with the increased size; a sixth step of increasing the size of the interval by the variable width from the representative value toward the upper limit, and computing a second probability that the amount of the material is within the interval with the increased size; and a seventh step of fixing the size of the interval set in the fifth step if the first probability is greater than or equal to the second probability, or fixing the size of the interval set in the sixth step if the first probability is less than the second probability, wherein: the fourth step, the fifth step, the sixth step, and the seventh step are repeated until the probability that the amount of the material is within the interval becomes greater than or equal to the target probability, the lower limit tolerance is the lower limit of the interval at a time point when the probability that the amount of the material is within the interval becomes greater than or equal to the target probability, and the upper limit tolerance is the upper limit of the interval at a time point when the probability that the amount of the material is within the interval becomes greater than or equal to the target probability; and wherein the material is nucleic acid molecules. calculating the probability distribution, wherein the calculating the probability distribution includes computing the probability distribution using a performance property of the liquid droplet ejection apparatus configured to inject the material into the container, a first step of setting the target probability; a second step of computing the representative value of the amount of material on the probability distribution; a third step of setting an interval of a lower limit to an upper limit including the representative value on the probability distribution; a fourth step of setting a variable width of the interval; a fifth step of increasing a size of the interval by the variable width from the representative value toward the lower limit, and computing a first probability that the amount of the material is within the interval with the increased size; a sixth step of increasing the size of the interval by the variable width from the representative value toward the upper limit, and computing a second probability that the amount of the material is within the interval with the increased size; and a seventh step of fixing the size of the interval set in the fifth step if the first probability is greater than or equal to the second probability, or fixing the size of the interval set in the sixth step if the first probability is less than the second probability, wherein: the fourth step, the fifth step, the sixth step, and the seventh step are repeated until the probability that the amount of the material is within the interval becomes greater than or equal to the target probability, the lower limit tolerance is the lower limit of the interval at a time point when the probability that the amount of the material is within the interval becomes greater than or equal to the target probability, and the upper limit tolerance is the upper limit of the interval at a time point when the probability that the amount of the material is within the interval becomes greater than or equal to the target probability; and wherein the material is nucleic acid molecules. the fourth step, the fifth step, the sixth step, and the seventh step are repeated until the probability that the amount of the material is within the interval becomes greater than or equal to the target probability, the lower limit tolerance is the lower limit of the interval at a time point when the probability that the amount of the material is within the interval becomes greater than or equal to the target probability, and the upper limit tolerance is the upper limit of the interval at a time point when the probability that the amount of the material is within the interval becomes greater than or equal to the target probability; and wherein the material is nucleic acid molecules.