A method for purifying or enriching a biomolecule, wherein the method comprises: (a) providing an aqueous solution comprising the biomolecule; (b) contacting the aqueous solution with a multimodal resin; (c) optionally washing the multimodal resin with an aqueous washing buffer; (d) eluting the biomolecule from the multimodal resin by using an aqueous buffer solution, wherein the aqueous buffer solution comprises: (i) at least 15 mM of 2-(N-morpholino)ethanesulfonic acid or 3-(N-morpholino)propanesulfonic acid (MOPS); (ii) between 0 and 400 mM in total concentration of one or more amino acids selected from the group consisting of alanine, arginine, asparagine, aspartic acid, cysteine, glutamic acid, glutamine, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, and valine; (iii) between 0 and 400 mM in total concentration of urea, guanidinium chloride, guanidinium thiocyanate, ammonium chloride, ammonium sulfate, ammonium phosphate, ammonium acetate, sodium chloride, sodium citrate, sodium caprylate, sodium phosphate, sodium iodide, potassium chloride, potassium citrate, potassium sulfate, potassium phosphate, potassium iodide, magnesium chloride, magnesium sulfate, magnesium phosphate, calcium chloride, calcium sulfate, calcium phosphate, or taurine; and (iv) between 0% and 60% (v/v) of ethylene glycol or propylene glycol or a mixture of ethylene glycol and propylene glycol; provided that the aqueous buffer solution comprises (a) an amino acid and/or (b) ethylene glycol and/or propylene glycol; and (e) collecting biomolecule containing fractions, thereby obtaining the biomolecule in purified or enriched form wherein said biomolecule is an enzyme. (a) providing an aqueous solution comprising the biomolecule; (b) contacting the aqueous solution with a multimodal resin; (c) optionally washing the multimodal resin with an aqueous washing buffer; (d) eluting the biomolecule from the multimodal resin by using an aqueous buffer solution, wherein the aqueous buffer solution comprises: (i) at least 15 mM of 2-(N-morpholino)ethanesulfonic acid or 3-(N-morpholino)propanesulfonic acid (MOPS); (ii) between 0 and 400 mM in total concentration of one or more amino acids selected from the group consisting of alanine, arginine, asparagine, aspartic acid, cysteine, glutamic acid, glutamine, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, and valine; (iii) between 0 and 400 mM in total concentration of urea, guanidinium chloride, guanidinium thiocyanate, ammonium chloride, ammonium sulfate, ammonium phosphate, ammonium acetate, sodium chloride, sodium citrate, sodium caprylate, sodium phosphate, sodium iodide, potassium chloride, potassium citrate, potassium sulfate, potassium phosphate, potassium iodide, magnesium chloride, magnesium sulfate, magnesium phosphate, calcium chloride, calcium sulfate, calcium phosphate, or taurine; and (iv) between 0% and 60% (v/v) of ethylene glycol or propylene glycol or a mixture of ethylene glycol and propylene glycol; provided that the aqueous buffer solution comprises (a) an amino acid and/or (b) ethylene glycol and/or propylene glycol; and wherein the aqueous buffer solution comprises: (i) at least 15 mM of 2-(N-morpholino)ethanesulfonic acid or 3-(N-morpholino)propanesulfonic acid (MOPS); (ii) between 0 and 400 mM in total concentration of one or more amino acids selected from the group consisting of alanine, arginine, asparagine, aspartic acid, cysteine, glutamic acid, glutamine, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, and valine; (iii) between 0 and 400 mM in total concentration of urea, guanidinium chloride, guanidinium thiocyanate, ammonium chloride, ammonium sulfate, ammonium phosphate, ammonium acetate, sodium chloride, sodium citrate, sodium caprylate, sodium phosphate, sodium iodide, potassium chloride, potassium citrate, potassium sulfate, potassium phosphate, potassium iodide, magnesium chloride, magnesium sulfate, magnesium phosphate, calcium chloride, calcium sulfate, calcium phosphate, or taurine; and (iv) between 0% and 60% (v/v) of ethylene glycol or propylene glycol or a mixture of ethylene glycol and propylene glycol; (i) at least 15 mM of 2-(N-morpholino)ethanesulfonic acid or 3-(N-morpholino)propanesulfonic acid (MOPS); (ii) between 0 and 400 mM in total concentration of one or more amino acids selected from the group consisting of alanine, arginine, asparagine, aspartic acid, cysteine, glutamic acid, glutamine, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, and valine; (iii) between 0 and 400 mM in total concentration of urea, guanidinium chloride, guanidinium thiocyanate, ammonium chloride, ammonium sulfate, ammonium phosphate, ammonium acetate, sodium chloride, sodium citrate, sodium caprylate, sodium phosphate, sodium iodide, potassium chloride, potassium citrate, potassium sulfate, potassium phosphate, potassium iodide, magnesium chloride, magnesium sulfate, magnesium phosphate, calcium chloride, calcium sulfate, calcium phosphate, or taurine; and (iv) between 0% and 60% (v/v) of ethylene glycol or propylene glycol or a mixture of ethylene glycol and propylene glycol; provided that the aqueous buffer solution comprises (a) an amino acid and/or (b) ethylene glycol and/or propylene glycol; and (e) collecting biomolecule containing fractions, thereby obtaining the biomolecule in purified or enriched form