Portable device for detecting biological hazard agents in a sample comprising: - a container enclosing a first housing filled with a culture medium for biological hazard agents, - a second housing for receiving the sample, - a lid that can be coupled to the container for closing said second housing, - means for (fluid)-communicating the first and the second housing so that an enriched mixture of the sample and the culture media is produced, that allows obtaining a sample ready for testing from said enriched mixture; and - means for sensing the presence of biological hazard agents in the sample ready for testing, characterised in that: - the second housing is configured and arranged for the deposition of the solid macro-sample on a bottom wall thereon, and has an inlet surface greater than 10 cm 2 for receiving the solid macro-sample to be deposited on said bottom wall of the second housing, and the second housing comprises a lateral wall provided with at least one visual indicator corresponding to an indication of a predetermined filling volume to be filled with the solid macro-sample, thus guarantying that the deposited sample quantity is appropriate for the culture medium volume; - the portable device further comprises a confined receptacle configured and arranged for receiving and confining an aliquot of a fine filtered representation of said enriched mixture, wherein said aliquot is predetermined by the dimensions of the confined receptacle, said confined receptacle being configured and arranged for enabling the extraction and detection of said predetermined aliquot, so that the portable device is configured, by comprising said confined receptacle, for detecting biological hazard agents in said predetermined aliquot of said enriched mixture of the solid macro-sample and culture media confined in said confined receptacle, such that it is possible to collect in a safe and easy way a macro-sample, to prepare it and detect the presence of biological hazard agents in a safe way. - a container enclosing a first housing filled with a culture medium for biological hazard agents, - a second housing for receiving the sample, - a lid that can be coupled to the container for closing said second housing, - means for (fluid)-communicating the first and the second housing so that an enriched mixture of the sample and the culture media is produced, that allows obtaining a sample ready for testing from said enriched mixture; and - means for sensing the presence of biological hazard agents in the sample ready for testing, - the second housing is configured and arranged for the deposition of the solid macro-sample on a bottom wall thereon, and has an inlet surface greater than 10 cm 2 for receiving the solid macro-sample to be deposited on said bottom wall of the second housing, and the second housing comprises a lateral wall provided with at least one visual indicator corresponding to an indication of a predetermined filling volume to be filled with the solid macro-sample, thus guarantying that the deposited sample quantity is appropriate for the culture medium volume; - the portable device further comprises a confined receptacle configured and arranged for receiving and confining an aliquot of a fine filtered representation of said enriched mixture, wherein said aliquot is predetermined by the dimensions of the confined receptacle, said confined receptacle being configured and arranged for enabling the extraction and detection of said predetermined aliquot, so that the portable device is configured, by comprising said confined receptacle, for detecting biological hazard agents in said predetermined aliquot of said enriched mixture of the solid macro-sample and culture media confined in said confined receptacle,