A portable cold and hot water supply device, comprising a container body, a controller, a power supply device and a thermoelectric semiconductor chip, wherein: the container body comprises a fluid inlet, a fluid outlet, a first chamber connected to the fluid inlet and comprising a circular connecting hole, a second chamber connected to the fluid outlet, and a valve sphere, wherein the first chamber is received in the second chamber, the second chamber connects to the first chamber via the circular connecting hole of the first chamber, the diameter of the valve sphere exceeds that of the circular connecting hole of the first chamber, and the valve sphere is rotatably disposed between the first and second chambers (113 and 114) to be detachably engaged in the circular connecting hole of the first chamber for controlling connection between the first and second chambers (113 and 114); the power supply device is electrically connected to the controller; and the thermoelectric semiconductor chip is abutted the second chamber of the container body and is electrically connected to the power supply device, wherein the controller drives the power supply device to energize the thermoelectric semiconductor chip for heating the fluid received in the second chamber when a temperature of a fluid received in the second chamber is less than a desired temperature from the controller, and the temperature of the fluid received in the second chamber is cooled via the thermoelectric semiconductor chip by expelling heat generating from the thermoelectric semiconductor chip when the temperature of the fluid received in the second chamber is greater than the desired temperature from the controller. the container body comprises a fluid inlet, a fluid outlet, a first chamber connected to the fluid inlet and comprising a circular connecting hole, a second chamber connected to the fluid outlet, and a valve sphere, wherein the first chamber is received in the second chamber, the second chamber connects to the first chamber via the circular connecting hole of the first chamber, the diameter of the valve sphere exceeds that of the circular connecting hole of the first chamber, and the valve sphere is rotatably disposed between the first and second chambers (113 and 114) to be detachably engaged in the circular connecting hole of the first chamber for controlling connection between the first and second chambers (113 and 114); the power supply device is electrically connected to the controller; and the thermoelectric semiconductor chip is abutted the second chamber of the container body and is electrically connected to the power supply device, wherein the controller drives the power supply device to energize the thermoelectric semiconductor chip for heating the fluid received in the second chamber when a temperature of a fluid received in the second chamber is less than a desired temperature from the controller, and the temperature of the fluid received in the second chamber is cooled via the thermoelectric semiconductor chip by expelling heat generating from the thermoelectric semiconductor chip when the temperature of the fluid received in the second chamber is greater than the desired temperature from the controller.