An aseptic fluid coupling system (500; 1200) comprising: a first coupling portion (300; 1000) comprising a first valve body (110; 810) defining a first longitudinal axis (102; 802) and a first end port (114; 814), a first valve member (120; 820) disposed within the first valve body, and a cap (200; 900) that is releasably coupleable to the first valve body, the cap enclosing at least a portion of the first valve body while the cap is coupled to the first valve body; and a second coupling portion (400; 1100) configured to releasably couple with the first coupling portion, comprising a housing (410; 1110) that defines a first longitudinal housing axis (402; 1102) and a second longitudinal housing axis (404; 1104), a second valve body (450; 1150) coupled with the housing and defining a second end port (454; 1154), a second valve member (460; 1160) disposed within the second valve body,and a connection member (420; 1120) configured to releasably couple with the first coupling portion (300; 1000), the connection member coupled with the housing (410; 1110) and movable between a first position that is coaxial with the first longitudinal housing axis (402; 1102) and a second position that is coaxial with the second longitudinal housing axis (404; 1104), and a flexible member (440; 1140) coupled to the housing (410; 1110) and to the connection member (420; 1120), wherein the flexible member reconfigures while the connection member (420; 1120) is moved between the first position and the second position, wherein the flexible member (440; 1140) is configured to maintain a sterile seal of the second coupling portion (400; 1100) while the connection member (420; 1120) is moved between the first position and the second position, wherein, while the connection member (420; 1120) is in the second position, the second valve body (450; 1150) is slidable along the second longitudinal housing axis (404; 1104) such that the first valve member (120; 820) can become engaged with the second valve member (460; 160) to thereby open a sterile fluid pathway between the first and second end ports (114, 454; 814, 1154). a first coupling portion (300; 1000) comprising a first valve body (110; 810) defining a first longitudinal axis (102; 802) and a first end port (114; 814), a first valve member (120; 820) disposed within the first valve body, and a cap (200; 900) that is releasably coupleable to the first valve body, the cap enclosing at least a portion of the first valve body while the cap is coupled to the first valve body; and a first valve body (110; 810) defining a first longitudinal axis (102; 802) and a first end port (114; 814), a first valve member (120; 820) disposed within the first valve body, and a cap (200; 900) that is releasably coupleable to the first valve body, the cap enclosing at least a portion of the first valve body while the cap is coupled to the first valve body; and a second coupling portion (400; 1100) configured to releasably couple with the first coupling portion, comprising a housing (410; 1110) that defines a first longitudinal housing axis (402; 1102) and a second longitudinal housing axis (404; 1104), a second valve body (450; 1150) coupled with the housing and defining a second end port (454; 1154), a second valve member (460; 1160) disposed within the second valve body,and a connection member (420; 1120) configured to releasably couple with the first coupling portion (300; 1000), the connection member coupled with the housing (410; 1110) and movable between a first position that is coaxial with the first longitudinal housing axis (402; 1102) and a second position that is coaxial with the second longitudinal housing axis (404; 1104), and a flexible member (440; 1140) coupled to the housing (410; 1110) and to the connection member (420; 1120), wherein the flexible member reconfigures while the connection member (420; 1120) is moved between the first position and the second position, wherein the flexible member (440; 1140) is configured to maintain a sterile seal of the second coupling portion (400; 1100) while the connection member (420; 1120) is moved between the first position and the second position, a housing (410; 1110) that defines a first longitudinal housing axis (402; 1102) and a second longitudinal housing axis (404; 1104), a second valve body (450; 1150) coupled with the housing and defining a second end port (454; 1154), a second valve member (460; 1160) disposed within the second valve body,and a connection member (420; 1120) configured to releasably couple with the first coupling portion (300; 1000), the connection member coupled with the housing (410; 1110) and movable between a first position that is coaxial with the first longitudinal housing axis (402; 1102) and a second position that is coaxial with the second longitudinal housing axis (404; 1104), and a flexible member (440; 1140) coupled to the housing (410; 1110) and to the connection member (420; 1120), wherein the flexible member reconfigures while the connection member (420; 1120) is moved between the first position and the second position, wherein the flexible member (440; 1140) is configured to maintain a sterile seal of the second coupling portion (400; 1100) while the connection member (420; 1120) is moved between the first position and the second position, wherein, while the connection member (420; 1120) is in the second position, the second valve body (450; 1150) is slidable along the second longitudinal housing axis (404; 1104) such that the first valve member (120; 820) can become engaged with the second valve member (460; 160) to thereby open a sterile fluid pathway between the first and second end ports (114, 454; 814, 1154).