Method for evaluating the amplification efficiency and/or the presence of inhibitors and/or degradation and/or performing a quantification of a nucleic acid in a real-time amplification reaction comprising: a. amplifying in a reaction composition a first target nucleic acid using a first primer pair in a real-time amplification reaction, b. amplifying in said reaction composition one or more internal nucleic acid control templates with a length of between 50 and 2000 nucleotides, preferably between 200 and 600 nucleotides, wherein the internal nucleic acid control template has a sequence selected from the group of: i. SEQ ID NO. 1 or a sequence that differs by no more than 15% in its sequence identity therefrom, ii. a reverse complement of SEQ ID NO. 1 or a sequence that differs by no more than 15% in its sequence identity therefrom, iii. a sequence or a reverse complement thereof comprising the final 18 to 30 3'-nucleotides of SEQ I D NO. 1 at its terminal 3'-end and the final 18 to 30 5'-nucleotides of SEQ I D NO. 1 at its terminal 5' - end, wherein between these terminal 3'-ends and 5'-ends the nucleic may have any nucleotide sequence and is between about 50 and about 1000 nucleotides in length, wherein the amplification reaction is a real-time PCR reaction, wherein a defined amount of IC nucleic acid is added to the reaction and the expected amplification product amount usually obtained in the absence of an amplification inhibitor is compared to the product amount obtained in the reaction, wherein the primers used for amplifying the internal nucleic acid control templates have a nucleotide sequence according to SEQ ID NO. 2 and 3, or the reverse complement thereof or at least have the first fifteen 3'-prime nucleotides in common with the sequences according to SEQ ID NO. 2 and 3 and the oligonucleotide probe used for detecting the amplification product has a sequence according to SEQ ID NO. 4. a. amplifying in a reaction composition a first target nucleic acid using a first primer pair in a real-time amplification reaction, b. amplifying in said reaction composition one or more internal nucleic acid control templates with a length of between 50 and 2000 nucleotides, preferably between 200 and 600 nucleotides, wherein the internal nucleic acid control template has a sequence selected from the group of: i. SEQ ID NO. 1 or a sequence that differs by no more than 15% in its sequence identity therefrom, ii. a reverse complement of SEQ ID NO. 1 or a sequence that differs by no more than 15% in its sequence identity therefrom, iii. a sequence or a reverse complement thereof comprising the final 18 to 30 3'-nucleotides of SEQ I D NO. 1 at its terminal 3'-end and the final 18 to 30 5'-nucleotides of SEQ I D NO. 1 at its terminal 5' - end, wherein between these terminal 3'-ends and 5'-ends the nucleic may have any nucleotide sequence and is between about 50 and about 1000 nucleotides in length, i. SEQ ID NO. 1 or a sequence that differs by no more than 15% in its sequence identity therefrom, ii. a reverse complement of SEQ ID NO. 1 or a sequence that differs by no more than 15% in its sequence identity therefrom, iii. a sequence or a reverse complement thereof comprising the final 18 to 30 3'-nucleotides of SEQ I D NO. 1 at its terminal 3'-end and the final 18 to 30 5'-nucleotides of SEQ I D NO. 1 at its terminal 5' - end, wherein between these terminal 3'-ends and 5'-ends the nucleic may have any nucleotide sequence and is between about 50 and about 1000 nucleotides in length, wherein the amplification reaction is a real-time PCR reaction, wherein a defined amount of IC nucleic acid is added to the reaction and the expected amplification product amount usually obtained in the absence of an amplification inhibitor is compared to the product amount obtained in the reaction, wherein the primers used for amplifying the internal nucleic acid control templates have a nucleotide sequence according to SEQ ID NO. 2 and 3, or the reverse complement thereof or at least have the first fifteen 3'-prime nucleotides in common with the sequences according to SEQ ID NO. 2 and 3 and the oligonucleotide probe used for detecting the amplification product has a sequence according to SEQ ID NO. 4.