A polypeptide composition comprising a polypeptide (d) consisting of 80 to 450 amino acid residues, the polypeptide (d) comprising a first region and a second region, the first region being represented by any one of the following amino acid sequences (1-i) to (1-iii): (1-i) an amino acid sequence consisting of the 1st to 44th amino acid residues of an amino acid sequence represented by SEQ ID NO: 1, (1-ii) an amino acid sequence consisting of an amino acid sequence that has identity of equal to or higher than 80% with an amino acid sequence including the amino acid sequence (1-i), and having a cell adhesion ability with respect to pluripotent stem cells, and (1-iii) an amino acid sequence consisting of an amino acid sequence that is formed by deletion, substitution, or addition of from 1 to 5 amino acids in the amino acid sequence (1-i), and having a cell adhesion ability with respect to pluripotent stem cells, the second region being represented by any one of the following amino acid sequences (2-i) to (2-iii): (2-i) an amino acid sequence represented by PRPSLAKKQRFRHRNRKGYRSQRGHSRGRNQN (SEQ ID NO: 3), (2-ii) an amino acid sequence which has identity of equal to or higher than 80% with the amino acid sequence represented by SEQ ID NO: 3, and has adsorbability with respect to a cell culture surface of a support, and (2-iii) an amino acid sequence that is formed by deletion, substitution, or addition of from 1 to 5 amino acids in the amino acid sequence represented by SEQ ID NO: 3, and has adsorbability with respect to a cell culture surface of a support, wherein the polypeptide (d) does not include the 56 th to 268 th amino acid residues in the amino acid sequence represented by SEQ ID NO:1, and an amount of a multimeric polypeptide, which is the polypeptide (d) and composed of two or more monomers held together by intermolecular cross-linking via cysteine residues included in the first region, being equal to or less than 20% by mass of a total mass of polypeptides contained in the composition. (1-i) an amino acid sequence consisting of the 1st to 44th amino acid residues of an amino acid sequence represented by SEQ ID NO: 1, (1-ii) an amino acid sequence consisting of an amino acid sequence that has identity of equal to or higher than 80% with an amino acid sequence including the amino acid sequence (1-i), and having a cell adhesion ability with respect to pluripotent stem cells, and (1-iii) an amino acid sequence consisting of an amino acid sequence that is formed by deletion, substitution, or addition of from 1 to 5 amino acids in the amino acid sequence (1-i), and having a cell adhesion ability with respect to pluripotent stem cells, (2-i) an amino acid sequence represented by PRPSLAKKQRFRHRNRKGYRSQRGHSRGRNQN (SEQ ID NO: 3), (2-ii) an amino acid sequence which has identity of equal to or higher than 80% with the amino acid sequence represented by SEQ ID NO: 3, and has adsorbability with respect to a cell culture surface of a support, and (2-iii) an amino acid sequence that is formed by deletion, substitution, or addition of from 1 to 5 amino acids in the amino acid sequence represented by SEQ ID NO: 3, and has adsorbability with respect to a cell culture surface of a support,