A method comprising: imaging a sample using light-sheet imaging, the light-sheet imaging including: generating light, and forming one or more light sheets from the generated light at one or more positions within the sample along respective illumination directions that are parallel with an illumination axis, and recording images of the sample from fluorescence emitted along a detection direction from the sample due to the optical interaction between the one or more light sheets and the sample, the detection direction being parallel with a detection axis; probing the light-sheet imaging by perturbing operating parameters associated with recording the images of the sample during light-sheet imaging including perturbing a focal plane at which the images of the sample are recorded; measuring one or more properties relating to how the light-sheet imaging is impacted by the probing including measuring properties related to the recorded images of the sample taken during probing; analyzing the one or more measured properties; determining, based on the analysis of the one or more measured properties, whether to adjust one or more operating parameters associated with the light-sheet imaging; and automatically adjusting, based on the determination, one or more operating parameters associated with the light-sheet imaging based on the analysis of the one or more measured properties. imaging a sample using light-sheet imaging, the light-sheet imaging including: generating light, and forming one or more light sheets from the generated light at one or more positions within the sample along respective illumination directions that are parallel with an illumination axis, and recording images of the sample from fluorescence emitted along a detection direction from the sample due to the optical interaction between the one or more light sheets and the sample, the detection direction being parallel with a detection axis; generating light, and forming one or more light sheets from the generated light at one or more positions within the sample along respective illumination directions that are parallel with an illumination axis, and recording images of the sample from fluorescence emitted along a detection direction from the sample due to the optical interaction between the one or more light sheets and the sample, the detection direction being parallel with a detection axis; probing the light-sheet imaging by perturbing operating parameters associated with recording the images of the sample during light-sheet imaging including perturbing a focal plane at which the images of the sample are recorded; measuring one or more properties relating to how the light-sheet imaging is impacted by the probing including measuring properties related to the recorded images of the sample taken during probing; analyzing the one or more measured properties; determining, based on the analysis of the one or more measured properties, whether to adjust one or more operating parameters associated with the light-sheet imaging; and automatically adjusting, based on the determination, one or more operating parameters associated with the light-sheet imaging based on the analysis of the one or more measured properties.