An anti-evaporation tube for use with an in vitro diagnostics automation system comprising: an anti-evaporation tube body for automatic insertion in a container (102/202) and to limit evaporation of one or more fluids in the container (102/202); and an alignment portion disposed on the anti-evaporation tube body and having a plurality of self-alignment components, the self-alignment components comprising: a plurality of flexible portions extending vertically away from a top of the anti-evaporation tube body; and a plurality of slots alternating between the plurality of flexible portions; wherein the plurality of flexible portions is able to move from an initial position to facilitate insertion of the alignment portion through an opening of the container (102/202) and limit movement of the anti-evaporation tube body within the container (102/202) characterized in that the plurality of self-alignment components further automatically align the anti-evaporation tube body to prevent an aspiration component from contacting the anti-evaporation tube during operation, wherein said slots between the movable alignment components of the anti-evaporation tube are configured for flexing and deflexing to provide ventilation; and the plurality of self-alignment components comprises one or more protrusions to be received by opposing recesses in the container (102/202); and the slots are positioned relative to a neck of the container such that a slot cross section is reduced by the neck of the container. an anti-evaporation tube body for automatic insertion in a container (102/202) and to limit evaporation of one or more fluids in the container (102/202); and an alignment portion disposed on the anti-evaporation tube body and having a plurality of self-alignment components, the self-alignment components comprising: a plurality of flexible portions extending vertically away from a top of the anti-evaporation tube body; and a plurality of slots alternating between the plurality of flexible portions; wherein the plurality of flexible portions is able to move from an initial position to facilitate insertion of the alignment portion through an opening of the container (102/202) and limit movement of the anti-evaporation tube body within the container (102/202) a plurality of flexible portions extending vertically away from a top of the anti-evaporation tube body; and a plurality of slots alternating between the plurality of flexible portions; wherein the plurality of flexible portions is able to move from an initial position to facilitate insertion of the alignment portion through an opening of the container (102/202) and limit movement of the anti-evaporation tube body within the container (102/202) characterized in that the plurality of self-alignment components further automatically align the anti-evaporation tube body to prevent an aspiration component from contacting the anti-evaporation tube during operation, wherein said slots between the movable alignment components of the anti-evaporation tube are configured for flexing and deflexing to provide ventilation; and the plurality of self-alignment components comprises one or more protrusions to be received by opposing recesses in the container (102/202); and the slots are positioned relative to a neck of the container such that a slot cross section is reduced by the neck of the container.