A method of cloning stable stress tolerant superoxide dismutase having acitivity under stress parameters of high temperature, pressure and sub-zero temperature from diverse plant species, wherein the high temperature is in the range of 100 °C to 121 °C, the pressure is in the range of 14 pounds per square inch (psi) to 15 psi, and the sub-zero temperature is in the range of 0° to minus 10 °C, the method comprising: a. isolating the total RNA from leaf tissue and synthesizing its complementary DNA, b. designing and preparing universal primer sets of Cu/Zn SOD gene selected from the group consisting of Seq ID no.22-23, wherein SEQ ID No.22: Forward primer = 5'CAGGAAGGAGATGG(C/T)CCAAC(A/C)3' Reverse primer = 5'(C/T)TGAA(A/G)(A/G)CC(A/G)AT(G/A/C)CCACAAGC3', wherein i. the length of the forward primer (SEQ ID No.22F) is 21 mer and for the reverse primer (SEQ ID NO.22R) is 21 mer, ii. the G+C content is in the range of 38 to 62 %, iii. the Tm is in the range of 49 °C to 58 °C, iv. the annealing temperature is preferably in the range of 53 °C to 58 °C, and v. optimal annealing temperature for SOD detection about 55°C and SEQ ID No.23: Forward primer = 5'TC(A/T)AC(C/T)GG(G/A/T)CC(A/G)CA(C/T)TA(C/T)AAT3' Reverse primer = 5' (C/T)TGAA(A/G)(A/G)CC(A/G)AT(G/A/C)CCACAAGC3', wherein i. the length of the forward primer (SEQ ID No.23F) is 21 mer and for the reverse primer (SEQ ID NO.23R) is 21 mer, ii. the G+C content is in the range of 33 to 62 %, iii. the Tm is in the range of 47 °C to 56 °C, and the annealing temperature is preferably in the range of 52 °C to 58 °C and optimal annealing temperature for SOD detection about 55°C. c. amplifying Cu/Zn SOD gene using the primer set obtained in step b, d. ligating the amplified product obtained in step c into a vector to yield a recombinant plasmid, e. transforming the recombinant plasmid obtained in step d into suitable cell for heterologous expression, f. purifying the stable stress tolerant superoxide dismutase. a. isolating the total RNA from leaf tissue and synthesizing its complementary DNA, b. designing and preparing universal primer sets of Cu/Zn SOD gene selected from the group consisting of Seq ID no.22-23, wherein SEQ ID No.22: Forward primer = 5'CAGGAAGGAGATGG(C/T)CCAAC(A/C)3' Reverse primer = 5'(C/T)TGAA(A/G)(A/G)CC(A/G)AT(G/A/C)CCACAAGC3', wherein i. the length of the forward primer (SEQ ID No.22F) is 21 mer and for the reverse primer (SEQ ID NO.22R) is 21 mer, ii. the G+C content is in the range of 38 to 62 %, iii. the Tm is in the range of 49 °C to 58 °C, iv. the annealing temperature is preferably in the range of 53 °C to 58 °C, and v. optimal annealing temperature for SOD detection about 55°C and SEQ ID No.23: Forward primer = 5'TC(A/T)AC(C/T)GG(G/A/T)CC(A/G)CA(C/T)TA(C/T)AAT3' Reverse primer = 5' (C/T)TGAA(A/G)(A/G)CC(A/G)AT(G/A/C)CCACAAGC3', wherein i. the length of the forward primer (SEQ ID No.23F) is 21 mer and for the reverse primer (SEQ ID NO.23R) is 21 mer, ii. the G+C content is in the range of 33 to 62 %, iii. the Tm is in the range of 47 °C to 56 °C, and the annealing temperature is preferably in the range of 52 °C to 58 °C and optimal annealing temperature for SOD detection about 55°C. Forward primer = 5'CAGGAAGGAGATGG(C/T)CCAAC(A/C)3' Reverse primer = 5'(C/T)TGAA(A/G)(A/G)CC(A/G)AT(G/A/C)CCACAAGC3', i. the length of the forward primer (SEQ ID No.22F) is 21 mer and for the reverse primer (SEQ ID NO.22R) is 21 mer, ii. the G+C content is in the range of 38 to 62 %, iii. the Tm is in the range of 49 °C to 58 °C, iv. the annealing temperature is preferably in the range of 53 °C to 58 °C, and v. optimal annealing temperature for SOD detection about 55°C and SEQ ID No.23: Forward primer = 5'TC(A/T)AC(C/T)GG(G/A/T)CC(A/G)CA(C/T)TA(C/T)AAT3' Reverse primer = 5' (C/T)TGAA(A/G)(A/G)CC(A/G)AT(G/A/C)CCACAAGC3', Forward primer = 5'TC(A/T)AC(C/T)GG(G/A/T)CC(A/G)CA(C/T)TA(C/T)AAT3' Reverse primer = 5' (C/T)TGAA(A/G)(A/G)CC(A/G)AT(G/A/C)CCACAAGC3', i. the length of the forward primer (SEQ ID No.23F) is 21 mer and for the reverse primer (SEQ ID NO.23R) is 21 mer, ii. the G+C content is in the range of 33 to 62 %, iii. the Tm is in the range of 47 °C to 56 °C, and c. amplifying Cu/Zn SOD gene using the primer set obtained in step b, d. ligating the amplified product obtained in step c into a vector to yield a recombinant plasmid, e. transforming the recombinant plasmid obtained in step d into suitable cell for heterologous expression, f. purifying the stable stress tolerant superoxide dismutase.