A pipette tip coupling device and pipette tip assembly comprising: a pipette tip comprising a circumscribing sidewall with an open interior passage including an interior surface and extending along a central longitudinal axis of the pipette tip between an open proximal end and an open distal end of the pipette tip; a pipette tip coupling device comprising: a shank having a proximal end face and a widest shank portion with a shank diameter; a coupler body distal to the shank, the coupler body having a widest coupler body portion with a coupler body diameter; wherein the coupler body diameter is greater than the shank diameter; the shank having a distal stem portion; a distal end plate; and an open-ended interior circumscribing surface forming an open passageway extending longitudinally from the proximal end face through the distal end plate; an elastomeric element disposed around the distal stem portion and adjacent to the distal end plate; and a discrete segment assembly circumscribing the shank comprising: a plurality of discrete segments forming a segmented ring, wherein each of the plurality of discrete segments comprises a radially inward surface (1272, 1282) and a radially outward surface (1274, 1276); and a spring retainer radially circumscribing the radially outward surface (1274, 1276) of each of the plurality of discrete segments in the segmented ring; characterized in that: each of the plurality of discrete segments of the pipette tip coupling device abuts a first part of an inner surface of the circumscribing sidewall of the pipette tip for securing the pipette tip to the pipette tip coupling device, and wherein the elastomeric element of the pipette tip coupling device abuts a second part of the inner surface of the circumscribing sidewall of the pipette tip to form a seal between the pipette tip coupling device and the pipette tip. a pipette tip comprising a circumscribing sidewall with an open interior passage including an interior surface and extending along a central longitudinal axis of the pipette tip between an open proximal end and an open distal end of the pipette tip; a pipette tip coupling device comprising: a shank having a proximal end face and a widest shank portion with a shank diameter; a coupler body distal to the shank, the coupler body having a widest coupler body portion with a coupler body diameter; wherein the coupler body diameter is greater than the shank diameter; the shank having a distal stem portion; a distal end plate; and an open-ended interior circumscribing surface forming an open passageway extending longitudinally from the proximal end face through the distal end plate; an elastomeric element disposed around the distal stem portion and adjacent to the distal end plate; and a discrete segment assembly circumscribing the shank comprising: a plurality of discrete segments forming a segmented ring, wherein each of the plurality of discrete segments comprises a radially inward surface (1272, 1282) and a radially outward surface (1274, 1276); and a spring retainer radially circumscribing the radially outward surface (1274, 1276) of each of the plurality of discrete segments in the segmented ring; a shank having a proximal end face and a widest shank portion with a shank diameter; a coupler body distal to the shank, the coupler body having a widest coupler body portion with a coupler body diameter; wherein the coupler body diameter is greater than the shank diameter; the shank having a distal stem portion; a distal end plate; and an open-ended interior circumscribing surface forming an open passageway extending longitudinally from the proximal end face through the distal end plate; an elastomeric element disposed around the distal stem portion and adjacent to the distal end plate; and a discrete segment assembly circumscribing the shank comprising: a plurality of discrete segments forming a segmented ring, wherein each of the plurality of discrete segments comprises a radially inward surface (1272, 1282) and a radially outward surface (1274, 1276); and a spring retainer radially circumscribing the radially outward surface (1274, 1276) of each of the plurality of discrete segments in the segmented ring; a plurality of discrete segments forming a segmented ring, wherein each of the plurality of discrete segments comprises a radially inward surface (1272, 1282) and a radially outward surface (1274, 1276); and a spring retainer radially circumscribing the radially outward surface (1274, 1276) of each of the plurality of discrete segments in the segmented ring; characterized in that: each of the plurality of discrete segments of the pipette tip coupling device abuts a first part of an inner surface of the circumscribing sidewall of the pipette tip for securing the pipette tip to the pipette tip coupling device, and wherein the elastomeric element of the pipette tip coupling device abuts a second part of the inner surface of the circumscribing sidewall of the pipette tip to form a seal between the pipette tip coupling device and the pipette tip.