A method for amplifying by PCR and for detecting at least one target nucleotide sequence possibly present in a biological sample, preferably a sample extracted from an individual, said method comprising the following consecutive steps: a) providing a device comprising: - a channel having a section comprised between 0.01 mm and 10 mm, - a dipstick or test strip in fluidic communication with the channel, said dipstick or test strip comprising one or first complementary and specific probes of at least one first sequence portion of the amplified target nucleotide sequence(s) or i.e. at least one first molecule from a pair of different and complementary molecules, capable of binding a second molecule different from said pair, said second molecule being bound to the first sequence portion of the amplified target nucleotide sequence(s); - means for generating at least three areas of different and constant temperatures, at least two (or even three) areas being located at different locations of the channel and the third (or fourth) area being located at the dipstick or test strip, b) introducing into the channel a solution comprising the sample as well as the reagents for amplifying the target nucleotide sequence(s), c) generating amplified sequences and possibly marked with the target nucleotide sequence(s) by having the solution circulate according to constant dynamic flow in the channel, d) putting said amplified sequences in contact with the dipstick or test strip, and e) generating, by hybridization between the first probes and said amplified sequences, a complex forming a measurable detection signal. a) providing a device comprising: - a channel having a section comprised between 0.01 mm and 10 mm, - a dipstick or test strip in fluidic communication with the channel, said dipstick or test strip comprising one or first complementary and specific probes of at least one first sequence portion of the amplified target nucleotide sequence(s) or i.e. at least one first molecule from a pair of different and complementary molecules, capable of binding a second molecule different from said pair, said second molecule being bound to the first sequence portion of the amplified target nucleotide sequence(s); - means for generating at least three areas of different and constant temperatures, at least two (or even three) areas being located at different locations of the channel and the third (or fourth) area being located at the dipstick or test strip, - a channel having a section comprised between 0.01 mm and 10 mm, - a dipstick or test strip in fluidic communication with the channel, said dipstick or test strip comprising one or first complementary and specific probes of at least one first sequence portion of the amplified target nucleotide sequence(s) or i.e. at least one first molecule from a pair of different and complementary molecules, capable of binding a second molecule different from said pair, said second molecule being bound to the first sequence portion of the amplified target nucleotide sequence(s); - means for generating at least three areas of different and constant temperatures, at least two (or even three) areas being located at different locations of the channel and the third (or fourth) area being located at the dipstick or test strip, b) introducing into the channel a solution comprising the sample as well as the reagents for amplifying the target nucleotide sequence(s), c) generating amplified sequences and possibly marked with the target nucleotide sequence(s) by having the solution circulate according to constant dynamic flow in the channel, d) putting said amplified sequences in contact with the dipstick or test strip, and e) generating, by hybridization between the first probes and said amplified sequences, a complex forming a measurable detection signal.