A method for the isolation of at least one first particle of a given type by means of a microfluidic system, which comprises a feeding assembly and a microfluidic circuit having: at least one inlet; at least one first outlet; and at least one microfluidic channel, which is designed to fluidically connect the inlet and the first outlet; the microfluidic channel comprises at least one first segment, at least one second segment, which is arranged downstream of the first segment, and at least one third segment, which is arranged downstream of the second segment; the method comprises a feeding step, during which the feeding assembly feeds a sample, comprising at least one first particle and at least one second particle of a type different from the first particle, from the inlet to the first outlet along the microfluidic channel; and a trapping step, during which the first particle is trapped in and prevented from escaping from the second segment, while letting the second particle, which reaches at least the third segment, pass through the second segment; the microfluidic system comprises a movement device, which is configured to directly exert a selective force upon said first particle of a given type; the method further comprises a selection step, which is subsequent to the trapping step and during which the movement device directly exerts a force upon the first particle so as to substantially selectively move the first particle, relative to at least part of a further content of the microfluidic channel, along at least one part of a given path from the second segment downstream of the second segment itself; the given path extends inside said microfluidic circuit; wherein the microfluidic system comprises a collection area, which is fluidically connected to the microfluidic channel; during the selection step, the movement device moves the first particle substantially selectively from the microfluidic channel, from the second segment, to the collection area. the microfluidic channel comprises at least one first segment, at least one second segment, which is arranged downstream of the first segment, and at least one third segment, which is arranged downstream of the second segment; the method comprises a feeding step, during which the feeding assembly feeds a sample, comprising at least one first particle and at least one second particle of a type different from the first particle, from the inlet to the first outlet along the microfluidic channel; and a trapping step, during which the first particle is trapped in and prevented from escaping from the second segment, while letting the second particle, which reaches at least the third segment, pass through the second segment; the microfluidic system comprises a movement device, which is configured to directly exert a selective force upon said first particle of a given type; the method further comprises a selection step, which is subsequent to the trapping step and during which the movement device directly exerts a force upon the first particle so as to substantially selectively move the first particle, relative to at least part of a further content of the microfluidic channel, along at least one part of a given path from the second segment downstream of the second segment itself; the given path extends inside said microfluidic circuit; wherein the microfluidic system comprises a collection area, which is fluidically connected to the microfluidic channel; during the selection step, the movement device moves the first particle substantially selectively from the microfluidic channel, from the second segment, to the collection area.