A cannabinoid nano-micelle preparation, comprising cannabinoid and an amphiphilic polymer; wherein the content of the cannabinoid is 1-40% by weight, and the content of the amphiphilic polymer is 1-99%, and a preparation method of the preparation comprises the following steps: preparing a cannabinoid nano-micelle solution from cannabinoid and an amphiphilic polymer; diluting the micellar solution obtained in the step, and drying to obtain cannabinoid nano-micelle powder; and preparing the cannabinoid nano-micelle powder obtained in the step into the cannabinoid nano-micelle preparation; wherein the amphiphilic polymer is selected from one or a combination of two or more of vitamin E polyethylene glycol succinate, amphiphilic polyurethane, poloxamer, a polylactic acid-polyethylene glycol-polylactic acid triblock copolymer, a polyethylene glycol-polyacrylic acid block copolymer, a polyethylene glycol-polyaspartic acid block copolymer, a polyethylene glycol-poly(lactic-co-glycolic acid) block copolymer, a polyethylene glycol-polycaprolactone block copolymer, a polyethylene glycol-polylactic acid block copolymer and a polyethylene glycol-polystyrene block copolymer; wherein the drying in the step is atomization freeze drying, wherein an atomization mode is selected from one or a combination of two or more of pneumatic atomization, pressure atomization, centrifugal atomization and ultrasonic atomization; and wherein the preparation is an inhalation preparation. preparing a cannabinoid nano-micelle solution from cannabinoid and an amphiphilic polymer; diluting the micellar solution obtained in the step, and drying to obtain cannabinoid nano-micelle powder; and preparing the cannabinoid nano-micelle powder obtained in the step into the cannabinoid nano-micelle preparation; wherein the amphiphilic polymer is selected from one or a combination of two or more of vitamin E polyethylene glycol succinate, amphiphilic polyurethane, poloxamer, a polylactic acid-polyethylene glycol-polylactic acid triblock copolymer, a polyethylene glycol-polyacrylic acid block copolymer, a polyethylene glycol-polyaspartic acid block copolymer, a polyethylene glycol-poly(lactic-co-glycolic acid) block copolymer, a polyethylene glycol-polycaprolactone block copolymer, a polyethylene glycol-polylactic acid block copolymer and a polyethylene glycol-polystyrene block copolymer; wherein the drying in the step is atomization freeze drying, wherein an atomization mode is selected from one or a combination of two or more of pneumatic atomization, pressure atomization, centrifugal atomization and ultrasonic atomization; and wherein the preparation is an inhalation preparation. wherein the amphiphilic polymer is selected from one or a combination of two or more of vitamin E polyethylene glycol succinate, amphiphilic polyurethane, poloxamer, a polylactic acid-polyethylene glycol-polylactic acid triblock copolymer, a polyethylene glycol-polyacrylic acid block copolymer, a polyethylene glycol-polyaspartic acid block copolymer, a polyethylene glycol-poly(lactic-co-glycolic acid) block copolymer, a polyethylene glycol-polycaprolactone block copolymer, a polyethylene glycol-polylactic acid block copolymer and a polyethylene glycol-polystyrene block copolymer; wherein the drying in the step is atomization freeze drying, wherein an atomization mode is selected from one or a combination of two or more of pneumatic atomization, pressure atomization, centrifugal atomization and ultrasonic atomization; and wherein the preparation is an inhalation preparation.