A system for preparing dialysis treatment fluid, comprising: an integrally interconnected fluid circuit (112, 200, 310, 530) including a mixing container (102, 210, 406, 952) pre-connected to the fluid circuit, the mixing container and fluid circuit being sealed from an external environment; the fluid circuit including a valve network (200B, 200E, 416) having junctions and valve portions that mechanically interface with valve actuators to define selectable flow paths in the valve network; the valve network further including a pumping portion (137, 985) and a fluid inlet line (117, 164, 167, 171, 431) with a connector (181A, 186) for connection to a fluid source (101, 104, 260, 420), the fluid inlet line being fluidly connected to the mixing container by the pumping portion; the valve network (200B, 200E, 416) fluidly connecting the mixing container to the fluid inlet line (117, 164, 167, 171, 431); the fluid inlet line (117, 164, 167, 171, 431) having at least one in-line sterilizing filter that includes either a pair of spaced sterilizing filters (115A, 115B) or a testable sterilizing filter with an air-line, wherein the fluid inlet line (164, 167) is connected to at least two concentrate containers (262, 264), the concentrate containers having a capacity sufficient to store enough concentrate to perform multiple peritoneal dialysis treatments, where each treatment includes multiple fill/drain cycles; the system further comprising a manifold (174, 900) connected to the valve network (200B, 200E, 416) and defining at least some of said junctions, the manifold having two chambers (982, 981) defined by shape of a rigid housing that is sealed by a film, wherein regions of the film form pressure sensors (178, 981, 982) integrated into the manifold. an integrally interconnected fluid circuit (112, 200, 310, 530) including a mixing container (102, 210, 406, 952) pre-connected to the fluid circuit, the mixing container and fluid circuit being sealed from an external environment; the fluid circuit including a valve network (200B, 200E, 416) having junctions and valve portions that mechanically interface with valve actuators to define selectable flow paths in the valve network; the valve network further including a pumping portion (137, 985) and a fluid inlet line (117, 164, 167, 171, 431) with a connector (181A, 186) for connection to a fluid source (101, 104, 260, 420), the fluid inlet line being fluidly connected to the mixing container by the pumping portion; the valve network (200B, 200E, 416) fluidly connecting the mixing container to the fluid inlet line (117, 164, 167, 171, 431); the fluid inlet line (117, 164, 167, 171, 431) having at least one in-line sterilizing filter that includes either a pair of spaced sterilizing filters (115A, 115B) or a testable sterilizing filter with an air-line, wherein the fluid inlet line (164, 167) is connected to at least two concentrate containers (262, 264), the concentrate containers having a capacity sufficient to store enough concentrate to perform multiple peritoneal dialysis treatments, where each treatment includes multiple fill/drain cycles; the system further comprising a manifold (174, 900) connected to the valve network (200B, 200E, 416) and defining at least some of said junctions, the manifold having two chambers (982, 981) defined by shape of a rigid housing that is sealed by a film, wherein regions of the film form pressure sensors (178, 981, 982) integrated into the manifold.