An e-seal for security and traceability in transport comprising: - a lock body; - a flag; - a bolt firmly connected to the flag, said bolt comprising a free end for the insertion according to an insertion axis into said lock body for the closing of the e-seal, through an insertion hole of the lock body; - a first RFID tag adapted to emit an own identification signal and housed into said flag; - a second RFID tag (5, 6) located into said lock body and adapted to emit an own identification signal when activated; - activation means of said second RFID tag (5, 6) adapted to move from a non-activated position to an activated position, wherein the moving of said activation means from said non-activated position to said activated position causes a change of state of said second RFID tag (5, 6), said movement of said activation means being caused by the insertion of said bolt into said lock body; - tampering detection means located into said lock body, wherein the insertion of the bolt or the tampering tool inside said lock body causes the disengagement between said activation means and said tampering detection means and wherein the subsequent extraction of said bolt or the tampering tool from said lock body causes the movement of said tampering detection means from said free-hole position to said block-hole position, characterized in that the tampering detection means is configured to be movable from a free-hole position, and is configured to not intercept said insertion hole to a blocked-hole position, and to intercept the insertion hole in order to avoid the insertion of the bolt or a tampering tool inside said lock body, wherein in said non-activated position, the said activation means engages said tampering detection means in order to maintain the tampering detection means in the free-hole position. - a lock body; - a flag; - a bolt firmly connected to the flag, said bolt comprising a free end for the insertion according to an insertion axis into said lock body for the closing of the e-seal, through an insertion hole of the lock body; - a first RFID tag adapted to emit an own identification signal and housed into said flag; - a second RFID tag (5, 6) located into said lock body and adapted to emit an own identification signal when activated; - activation means of said second RFID tag (5, 6) adapted to move from a non-activated position to an activated position, wherein the moving of said activation means from said non-activated position to said activated position causes a change of state of said second RFID tag (5, 6), said movement of said activation means being caused by the insertion of said bolt into said lock body; - tampering detection means located into said lock body, wherein the insertion of the bolt or the tampering tool inside said lock body causes the disengagement between said activation means and said tampering detection means and wherein the subsequent extraction of said bolt or the tampering tool from said lock body causes the movement of said tampering detection means from said free-hole position to said block-hole position, characterized in that the tampering detection means is configured to be movable from a free-hole position, and is configured to not intercept said insertion hole to a blocked-hole position, and to intercept the insertion hole in order to avoid the insertion of the bolt or a tampering tool inside said lock body, wherein in said non-activated position, the said activation means engages said tampering detection means in order to maintain the tampering detection means in the free-hole position.