A proline hydroxylase, comprising: a protein having the amino acid sequence of SEQ ID NO: 2 with a mutation of one or more amino acids and having a proline hydroxylase activity, wherein the mutation is selected from the group consisting of: Y35W, Y35K, 139K, 139R, H119R, K123D, E27K+Y35W/K, E27K+I39K/R, E27K+K123D/I/Q/S, 139K/R+Y35W/K, 139K/R+K123D/1/Q/S, K123D+W40F, K123D+Y35W/K, E27K+I39K/R+K123D/I/Q/S, K123D/I/Q/S+N165H, E27K+Y35W/K+139K/R, E27K+S37C/E/F/N/W/T+I39K/R, E27K+E34N/G/L/D/S+I39K/R, E27K+139K/R+D30S, E27K+I39K/R+I118F/V/R, E27K+139K/R+198V, Y35W/K+S37C/E/F/N/W/T+W40F, S37C/E/F/N/W/T+I223V+K123D/I/Q/S, E27K+I39K+Y35W/K+S37C/E/F/N/W/T, E27K+I39K/R+S37C/E/F/N/W/T+K123D/I/Q/S, E27K+I39K/R+K106Q+K112E, E27K+I39K/R+Y35W/K+S37C/E/F/N/W/T+K123D/I/Q/S, E27K+I39K/R+S37C/E/F/N/W/T+I58A/C/R/S/V/Y, E27K+S37C/E/F/N/W/T+I223V+K123D/I/Q/S, S37C/E/F/N/W/T+I39K/R+I223V+K123D/I/Q/S, E27K+S37C/E/F/N/W/T+I39K/R+K123D/I/Q/S+I98V, E27K+S37C/E/F/N/W/T+I39K/R+K123D/I/Q/S+I223V, F26L+E27K+I39K/R+K123D/I/Q/S, 1223V+S37C/E/F/N/W/T+E27K+139K/R, E27K+S37C/E/F/N/W/T+I39K/R+I98V+K123D/I/Q/S+I223V, E27K+S37C/E/F/N/W/T+I39K/R+I58A/C/R/S/T/V/Y+K123D/I/Q/S, T25G/R+E27K+S37C/E/F/N/W/T+I39K/R+I58A/C/R/S/T/V/Y, E27K +S37C/E/F/N/W/T+I39K/R+I58A/C/R/S/T/V/Y+K86P, K123D/I/Q/S+Y35W/K+I120V, E27K+D30S+I39K/R+I58A/C/R/S/T/V/Y+K112E, S37C/E/F/N/W/T+139K/R+N 165H, E27K+E34N/G/L/D/S+I39K/R+I58A/C/R/S/T/V/Y+I223V, E27K+S37C/E/F/N/W/T+I39K/R+I58A/C/R/S/T/V/Y+D173G, E27K+S37C/E/F/N/W/T+I39K/R+I58A/C/R/S/T/V/Y+D173G+I118F/V/R, E27K+E34N/G/L/D/S+S37C/E/F/N/W/T+I39K/R+I58A/C/R/S/T/V/Y, E27K+E34N/G/L/D/S+S37C/E/F/N/W/T+I39K/R+I58A/C/R/S/T/V/Y+D173G, E27K+S37C/E/F/N/W/T+I39K/R+I58A/C/R/S/T/V/Y+D173G+K123D/I/Q/S, E27K+S37C/E/F/N/W/T+I39K/R+I58A/C/R/S/T/V/Y+D173G+K123D/I/Q/S+I11 8F/V/R, E27K+S37C/E/F/N/W/T+I39K/R+I58A/C/R/S/T/V/Y+D173G+K123D/I/Q/S+N1 65H, E27K+E34N/G/L/D/S+S37C/E/F/N/W/T+I39K/R+I58A/C/R/S/T/V/+D173G+K1 23D/I/Q/S, H14R+E27K+D30S+E34N/G/L/D/S+I39K/R+I98V+K106V/T/Q+K112E/R+I223 V, T25G/R+E27K+S37C/E/F/N/W/T+I39K/R+I58A/C/R/S/T/V/Y+D173G+K123D/I /Q/S+I118F/V/R+N165H, H 14R+E27K+E34N/G/L/D/S+139K/R+198V+K106V/T/Q+K112E/R+1223V, H14R+E27K+E34N/G/L/D/S+S37C/E/F/N/W/T+I39K/R+I98V+K106V/T/Q+K1 12E/R+1223V, H14R+E27K+E34N/G/L/D/S+Y35W/K+I39K/R+I98V+K106V/T/Q+K112E/R+I2 23V, H14R+E27K+E34N/G/L/D/S+I39K/R+I98V+K106V/T/Q+K112E/R+K123D/I/Q/ S+1223V, H14R+E27K+E34N/G/L/D+I39K/R+I58A/C/R/S/T/V/Y+I98V+K106V/T/Q+K112 E/R+1223V and H 14R+E27K+E34N/G/L/D/S+139K/R+198V+K106V/T/Q+K112E/R+1118F/V/R+ 1223V, wherein '/' represents 'or'. a protein having the amino acid sequence of SEQ ID NO: 2 with a mutation of one or more amino acids and having a proline hydroxylase activity, wherein the mutation is selected from the group consisting of: Y35W, Y35K, 139K, 139R, H119R, K123D, E27K+Y35W/K, E27K+I39K/R, E27K+K123D/I/Q/S, 139K/R+Y35W/K, 139K/R+K123D/1/Q/S, K123D+W40F, K123D+Y35W/K, E27K+I39K/R+K123D/I/Q/S, K123D/I/Q/S+N165H, E27K+Y35W/K+139K/R, E27K+S37C/E/F/N/W/T+I39K/R, E27K+E34N/G/L/D/S+I39K/R, E27K+139K/R+D30S, E27K+I39K/R+I118F/V/R, E27K+139K/R+198V, Y35W/K+S37C/E/F/N/W/T+W40F, S37C/E/F/N/W/T+I223V+K123D/I/Q/S, E27K+I39K+Y35W/K+S37C/E/F/N/W/T, E27K+I39K/R+S37C/E/F/N/W/T+K123D/I/Q/S, E27K+I39K/R+K106Q+K112E, E27K+I39K/R+Y35W/K+S37C/E/F/N/W/T+K123D/I/Q/S, E27K+I39K/R+S37C/E/F/N/W/T+I58A/C/R/S/V/Y, E27K+S37C/E/F/N/W/T+I223V+K123D/I/Q/S, S37C/E/F/N/W/T+I39K/R+I223V+K123D/I/Q/S, E27K+S37C/E/F/N/W/T+I39K/R+K123D/I/Q/S+I98V, E27K+S37C/E/F/N/W/T+I39K/R+K123D/I/Q/S+I223V, F26L+E27K+I39K/R+K123D/I/Q/S, 1223V+S37C/E/F/N/W/T+E27K+139K/R, E27K+S37C/E/F/N/W/T+I39K/R+I98V+K123D/I/Q/S+I223V, E27K+S37C/E/F/N/W/T+I39K/R+I58A/C/R/S/T/V/Y+K123D/I/Q/S, T25G/R+E27K+S37C/E/F/N/W/T+I39K/R+I58A/C/R/S/T/V/Y, E27K +S37C/E/F/N/W/T+I39K/R+I58A/C/R/S/T/V/Y+K86P, K123D/I/Q/S+Y35W/K+I120V, E27K+D30S+I39K/R+I58A/C/R/S/T/V/Y+K112E, S37C/E/F/N/W/T+139K/R+N 165H, E27K+E34N/G/L/D/S+I39K/R+I58A/C/R/S/T/V/Y+I223V, E27K+S37C/E/F/N/W/T+I39K/R+I58A/C/R/S/T/V/Y+D173G, E27K+S37C/E/F/N/W/T+I39K/R+I58A/C/R/S/T/V/Y+D173G+I118F/V/R, E27K+E34N/G/L/D/S+S37C/E/F/N/W/T+I39K/R+I58A/C/R/S/T/V/Y, E27K+E34N/G/L/D/S+S37C/E/F/N/W/T+I39K/R+I58A/C/R/S/T/V/Y+D173G, E27K+S37C/E/F/N/W/T+I39K/R+I58A/C/R/S/T/V/Y+D173G+K123D/I/Q/S, E27K+S37C/E/F/N/W/T+I39K/R+I58A/C/R/S/T/V/Y+D173G+K123D/I/Q/S+I11 8F/V/R, E27K+S37C/E/F/N/W/T+I39K/R+I58A/C/R/S/T/V/Y+D173G+K123D/I/Q/S+N1 65H, E27K+E34N/G/L/D/S+S37C/E/F/N/W/T+I39K/R+I58A/C/R/S/T/V/+D173G+K1 23D/I/Q/S, H14R+E27K+D30S+E34N/G/L/D/S+I39K/R+I98V+K106V/T/Q+K112E/R+I223 V, T25G/R+E27K+S37C/E/F/N/W/T+I39K/R+I58A/C/R/S/T/V/Y+D173G+K123D/I /Q/S+I118F/V/R+N165H, H 14R+E27K+E34N/G/L/D/S+139K/R+198V+K106V/T/Q+K112E/R+1223V, H14R+E27K+E34N/G/L/D/S+S37C/E/F/N/W/T+I39K/R+I98V+K106V/T/Q+K1 12E/R+1223V, H14R+E27K+E34N/G/L/D/S+Y35W/K+I39K/R+I98V+K106V/T/Q+K112E/R+I2 23V, H14R+E27K+E34N/G/L/D/S+I39K/R+I98V+K106V/T/Q+K112E/R+K123D/I/Q/ S+1223V, H14R+E27K+E34N/G/L/D+I39K/R+I58A/C/R/S/T/V/Y+I98V+K106V/T/Q+K112 E/R+1223V and H 14R+E27K+E34N/G/L/D/S+139K/R+198V+K106V/T/Q+K112E/R+1118F/V/R+ 1223V, wherein '/' represents 'or'.