mhurhangee/ep-ind-claims huggingface dataset:
A method for the transient expression of recombinant polypeptides and proteins using a permanent human amniocyte cell line comprising the following steps: a) transfection of the permanent human amniocyte cell line with a nucleic acid molecule comprising a nucleic acid sequence coding for a desired recombinant polypeptide or protein, and a recognition or binding site for the large T antigen of SV40 or the Epstein-Barr virus nuclear antigen 1 (EBNA1), b) cultivation of the transfected permanent human amniocyte cell line obtained in step a) under conditions that permit the expression of the desired recombinant polypeptide or protein, and subsequently c) isolation of the desired recoinbinant polypeptide or protein from the cells or the culture supernatant, wherein the permanent human amniocyte cell line was obtained by a method comprising: i) transfection of primary human amniocyte cells with a nucleic acid molecule comprising a nucleic acid sequence coding for the adenoviral gene functions E1A and E1B, and e) subsequent transfection of the permanent human amniocyte cell line obtained in step i) with a nucleic acid molecule comprising a nucleic acid sequence coding for the large T antigen of SV40 or the Epstein-Barr virus nuclear antigen 1 (EBNA1). a) transfection of the permanent human amniocyte cell line with a nucleic acid molecule comprising a nucleic acid sequence coding for a desired recombinant polypeptide or protein, and a recognition or binding site for the large T antigen of SV40 or the Epstein-Barr virus nuclear antigen 1 (EBNA1), b) cultivation of the transfected permanent human amniocyte cell line obtained in step a) under conditions that permit the expression of the desired recombinant polypeptide or protein, and subsequently c) isolation of the desired recoinbinant polypeptide or protein from the cells or the culture supernatant, i) transfection of primary human amniocyte cells with a nucleic acid molecule comprising a nucleic acid sequence coding for the adenoviral gene functions E1A and E1B, and e) subsequent transfection of the permanent human amniocyte cell line obtained in step i) with a nucleic acid molecule comprising a nucleic acid sequence coding for the large T antigen of SV40 or the Epstein-Barr virus nuclear antigen 1 (EBNA1).
A cutting blade, in particular a scythe-like blade or spiral blade or circular blade, for an apparatus for slicing food products, in particular for a highspeed slicer, the cutting blade rotating about an axis of rotation during a cutting operation, having a radially outer peripheral edge which acts as a blade edge and which has a curved extent about the axis of rotation; and having a plurality of cutting teeth which are arranged distributed following one another along the peripheral edge, wherein each cutting tooth has a blade edge which comprises a cutting surface and a cutting edge radially outwardly bounding the cutting surface; wherein the cutting edges lie in a common cutting plane; and wherein the cutting surfaces each extend inclined with respect to the cutting plane and intersect the cutting plane at a tilt angle, with the cutting edges forming the respective intersection line between the cutting surface and the cutting plane, wherein the respective cutting surfaces of two cutting teeth directly following one another are connected to one another by a transition surface, with the transition surface being formed as a recess projecting backwards with respect to the cutting surfaces, characterized in that each cutting surface is connected both to the cutting surface of a directly preceding cutting tooth and to the cutting surface of a directly following cutting tooth by one of the recesses.
A pylon assembly for a tiltrotor aircraft having a fuselage and first and second wings each having an outboard end and a pylon assembly positioned proximate an outboard end, the pylon assembly comprising: a rotor assembly operable to rotate between a generally vertical orientation, in a VTOL flight mode, and a generally horizontal orientation, in a forward flight mode, and having an intermediate orientation therebetween, in a conversion flight mode, the rotor assembly including a proprotor operable to produce a slipstream; a wing extension outboard of the rotor assembly, the wing extension having a forward edge and an outboard end; and a winglet coupled to the outboard end of the wing extension, the winglet having a forward edge and an outward-facing side; wherein the wing extension and the winglet form an angle of less than or equal to 90 degrees and the winglet is dihedral so as to minimize the forward-facing profile of the wing extension in the conversion flight mode, thereby reducing drag of the tiltrotor aircraft during forward motion in the conversion flight mode; wherein the wing extension and the winglet are operable to rotate with the rotor assembly such that the forward edges (109, 110) of the wing extension and the winglet remain in the slipstream of the proprotor. a rotor assembly operable to rotate between a generally vertical orientation, in a VTOL flight mode, and a generally horizontal orientation, in a forward flight mode, and having an intermediate orientation therebetween, in a conversion flight mode, the rotor assembly including a proprotor operable to produce a slipstream; a wing extension outboard of the rotor assembly, the wing extension having a forward edge and an outboard end; and a winglet coupled to the outboard end of the wing extension, the winglet having a forward edge and an outward-facing side; wherein the wing extension and the winglet form an angle of less than or equal to 90 degrees and the winglet is dihedral so as to minimize the forward-facing profile of the wing extension in the conversion flight mode, thereby reducing drag of the tiltrotor aircraft during forward motion in the conversion flight mode; wherein the wing extension and the winglet are operable to rotate with the rotor assembly such that the forward edges (109, 110) of the wing extension and the winglet remain in the slipstream of the proprotor.
Boot having an outer upper inside which an internal comfort element is inserted, said outer upper comprising a top part and a bottom part and comprising at least two portions that are mobile with respect to one another; tightening means being provided so as to bring the two mobile portions closer to one another in order to adapt the volume of the boot to the foot of the user, said tightening means being placed on the bottom part of the outer upper, at the top part of the instep, near the line of the short heel perimeter and said tightening means having external traction means of which a first end is fastened to a first portion of the outer upper and of which a second end is in relation with actuation means that are fastened to a second portion of the outer upper, characterized in that it also has internal traction means mainly placed beneath the outer upper so as to act on the internal comfort element in order to tighten the internal comfort element against the foot of the user, said internal traction means being in relation with the same actuation means.
A method of manufacturing a therapeutic protein, comprising: transfecting a cholesterol-auxotrophic and glutamine-auxotrophic cell with (i) a nucleic acid encoding a protein that restores cholesterol biosynthesis in the cell; (ii) a nucleic acid encoding a protein that restores glutamine biosynthesis in the cell; (iii) a nucleic acid comprising an aminoglycoside antibiotic resistance gene; and (iv) a nucleic acid encoding the therapeutic protein.
A method for correcting roundness or straightness errors on elongated workpieces clamped between centring means, with at least one toothed zone that defines a movement direction of the workpiece, such as on gear shafts or on gear racks, wherein the roundness or straightness errors are removed or reduced by correction between the centring means by means of straightening anvils and at least one straightening hammer in a straightening press, characterised in that - non-contacting measurement takes place, wherein at least one point-like or strip-like light beam with a directional component that points radially or across the direction of longitudinal extension of the workpiece is successively directed and imaged to several narrowly delimited area elements that in the direction of movement of the workpiece are situated along the toothed zone; - measurement light reflected by the area elements of the tooth arrangement is acquired by a light measuring device and is processed for measuring the distance of the space of the reflection surfaces of the tooth arrangement from a reference point; - the distances of the reflection surfaces from the reference point in various angular positions and/or positions of longitudinal extension of the workpiece are compared, and from this comparison roundness or straightness errors of the workpiece are determined. - non-contacting measurement takes place, wherein at least one point-like or strip-like light beam with a directional component that points radially or across the direction of longitudinal extension of the workpiece is successively directed and imaged to several narrowly delimited area elements that in the direction of movement of the workpiece are situated along the toothed zone; - measurement light reflected by the area elements of the tooth arrangement is acquired by a light measuring device and is processed for measuring the distance of the space of the reflection surfaces of the tooth arrangement from a reference point; - the distances of the reflection surfaces from the reference point in various angular positions and/or positions of longitudinal extension of the workpiece are compared, and from this comparison roundness or straightness errors of the workpiece are determined.
A catalytic converter for an internal combustion engine for obtaining energy from the combustion of fuel and air, wherein the resulting combustion gas is guided through the catalytic converter in a conducting path at which a high-temperature resistant diffusion membrane is attached which borders on a regrind collector which is maintained under a lower pressure (pR) than a general catalytic converter internal pressure (pC) and a regrind gas of the downstream internal combustion engine suctioned from the combustion gas is thus added as a supplementary combustible fuel, characterized in that a swirl chamber is inserted into the conducting path, said swirl chamber bordering on the diffusion membrane and in which the catalytic converter internal pressure (pC) is increased by obstacles for the combustion gas, and in that water is added to the combustion gas into the conducting path in front of the swirl chamber in a certain ratio to the burned fuel, and in that the diffusion membrane is formed from at least one grouping of a layer of a fine metal mesh that is surrounded by more coarse metal mesh, wherein the grouping is coated with palladium, and adjoins a porous impact membrane plate which delimits the swirl chamber.
An apparatus, comprising: an ultrasound probe, comprising: a plurality of modules including a first module and a second module, wherein each of the first and second modules (71, 72) comprises transmit circuitry, at least one ultrasound element, and receive circuitry, the ultrasound probe further comprising: a memory configured to store digital parameter data for programming programmable circuitry of the ultrasound probe; and a parameter loader (107, 504) coupling the memory to the first and second modules; wherein the parameter loader (107, 504) is configured to provide a packet of the digital parameter data from the memory to the first module, wherein the first module and second module are coupled to each other, and wherein the first module is configured to operate on the packet of the digital parameter data only if the packet is intended for the first module and to pass the packet of the digital parameter data from the first module to the second module without operating thereon if the packet is not intended for the first module. an ultrasound probe, comprising: a plurality of modules including a first module and a second module, wherein each of the first and second modules (71, 72) comprises transmit circuitry, at least one ultrasound element, and receive circuitry, the ultrasound probe further comprising: a memory configured to store digital parameter data for programming programmable circuitry of the ultrasound probe; and a parameter loader (107, 504) coupling the memory to the first and second modules; wherein the parameter loader (107, 504) is configured to provide a packet of the digital parameter data from the memory to the first module, wherein the first module and second module are coupled to each other, and wherein the first module is configured to operate on the packet of the digital parameter data only if the packet is intended for the first module and to pass the packet of the digital parameter data from the first module to the second module without operating thereon if the packet is not intended for the first module. a memory configured to store digital parameter data for programming programmable circuitry of the ultrasound probe; and a parameter loader (107, 504) coupling the memory to the first and second modules; wherein the parameter loader (107, 504) is configured to provide a packet of the digital parameter data from the memory to the first module, wherein the first module and second module are coupled to each other, and wherein the first module is configured to operate on the packet of the digital parameter data only if the packet is intended for the first module and to pass the packet of the digital parameter data from the first module to the second module without operating thereon if the packet is not intended for the first module.
A composition for the treatment of Acne Vulgaris that: (a) is administered in a single aqueous phase, in the form of a lotion, the rest of which is made up solely of water, and which does not include the use of additional oil, and that (b) consists by weight of: 3% to 7% of D-Panthenol, 3% to 7% of Niacinamide, 5% to 7% of Sulphacetamide Sodium, 3% to 7% Tea tree oil, 3% to 7% Licorice root extract, and 1% Boric Acid, and (c) in which the total weight of D-Panthenol, Niacinamide, Sulphacetamide Sodium, and Boric Acid equals or exceeds 12% of the composition.
Device for carrying out corrosion tests on steel materials, for example for testing for sulphide stress corrosion cracking according to the NACE Standard TM-0177-2005, consisting of a testing ring in which a specimen immersed in a testing solution can be subjected to a tensile force via a tensioning element and the specimen can be connected at one end to the testing ring and at the other end to the tensioning element; wherein - the testing ring is provided, in the region of a fixing location of the specimen, with an upper and a lower supporting plate (2, 2') at the outer circumference, the supporting plates (2, 2') being ground plane-parallel; - the tensioning element is provided, for introducing force into the specimen, with a tensioning head and a spherical bearing arranged on the upper supporting plate; - in the flow direction of the applied tensile force of the tensioning element, there is arranged between the spherical bearing and a ball bearing a force measuring device which directly measures the acting force; and - a further spherical bearing is arranged between the ball bearing and the tensioning head. - the testing ring is provided, in the region of a fixing location of the specimen, with an upper and a lower supporting plate (2, 2') at the outer circumference, the supporting plates (2, 2') being ground plane-parallel; - the tensioning element is provided, for introducing force into the specimen, with a tensioning head and a spherical bearing arranged on the upper supporting plate; - in the flow direction of the applied tensile force of the tensioning element, there is arranged between the spherical bearing and a ball bearing a force measuring device which directly measures the acting force; and - a further spherical bearing is arranged between the ball bearing and the tensioning head.
A cleaning sheet for being replaceably attached to a flat cleaning tool comprising: a cleaning surface for covering the bottom surface of the cleaning tool and locked to the upper surface of the cleaning tool by bending along longitudinal edge sections of the cleaning tool; and embossed sections compressed in the thickness direction and uncompressed non-embossed sections that are arranged alternately along bent sections folding along the longitudinal edge sections, characterized in that the embossed sections are arranged over the entire area of the cleaning sheet except areas in which the non-embossed sections are arranged, and the non-embossed sections arranged along the bent section corresponding to one of the longitudinal edge sections of the cleaning tool and the non-embossed sections arranged along the bent section corresponding to the other longitudinal edge sections of the cleaning tool are arranged in the different positions with respect to the extending direction of the bent sections. a cleaning surface for covering the bottom surface of the cleaning tool and locked to the upper surface of the cleaning tool by bending along longitudinal edge sections of the cleaning tool; and embossed sections compressed in the thickness direction and uncompressed non-embossed sections that are arranged alternately along bent sections folding along the longitudinal edge sections, characterized in that the embossed sections are arranged over the entire area of the cleaning sheet except areas in which the non-embossed sections are arranged, and the non-embossed sections arranged along the bent section corresponding to one of the longitudinal edge sections of the cleaning tool and the non-embossed sections arranged along the bent section corresponding to the other longitudinal edge sections of the cleaning tool are arranged in the different positions with respect to the extending direction of the bent sections.
An outdoor unit of an air conditioner, comprising: a body housing defining therein a heat exchanger room and a machine room that further defines two portions divided by a substantially vertical plane that includes a straight line passing a center of a bottom plate provided on a bottom of the machine room and extending in a depth direction of the machine room; a power-supply driving compressor being driven by an electric power; and a non-power-supply driving compressor unit containing a driving source other than the electric power and a non-power-supply driving compressor being driven by the driving source other than the electric power, wherein a substantially central position of the non-power-supply driving compressor unit is disposed in one of the two portions of the machine room, and a substantially central position of the power-supply driving compressor is disposed in the other one of the two portions of the machine room; and wherein a horizontal direction distance between a substantially central position of the non-power-supply driving compressor and the substantially central position of the power-supply driving compressor is shorter than a horizontal direction distance between the substantially central position of the non-power-supply driving compressor unit and the substantially central position of the power-supply driving compressor. a body housing defining therein a heat exchanger room and a machine room that further defines two portions divided by a substantially vertical plane that includes a straight line passing a center of a bottom plate provided on a bottom of the machine room and extending in a depth direction of the machine room; a power-supply driving compressor being driven by an electric power; and a non-power-supply driving compressor unit containing a driving source other than the electric power and a non-power-supply driving compressor being driven by the driving source other than the electric power, wherein a substantially central position of the non-power-supply driving compressor unit is disposed in one of the two portions of the machine room, and a substantially central position of the power-supply driving compressor is disposed in the other one of the two portions of the machine room; and
Method for controlling colours in a printing operation on a printing machine using a computer, the method comprising the following steps of - printing a test element with a selected number of colour measurement patches and measuring the printed colour measurement patches of the test element using a colour measuring device that takes spectral measurements, - calculating the colorimetric actual colour values on the basis of the measured colour values of the colour measurement patches of the test element and displaying selected actual colour values on a display device, - inputting colorimetric target colour values and calculating adapted spectral colour values on the basis of the colorimetric target colour values, - calculating densitometric, colorimetric or spectral actual tone values or actual dot gains on the basis of the calculated colorimetric actual colour values of a subset of the colour patches that have been measured in the test element by the spectral colour measurement device and displaying them on the display device, - inputting densitometric, colorimetric or spectral target tone values or target dot gains and calculating adapted spectral colour values by comparing with the calculated colorimetric actual colour values and - saving the adapted spectral colour values to calculate colour profiles, process calibrations and process control values and controlling the printing machine on the basis of the colour profiles, process calibrations and process control values. - printing a test element with a selected number of colour measurement patches and measuring the printed colour measurement patches of the test element using a colour measuring device that takes spectral measurements, - calculating the colorimetric actual colour values on the basis of the measured colour values of the colour measurement patches of the test element and displaying selected actual colour values on a display device, - inputting colorimetric target colour values and calculating adapted spectral colour values on the basis of the colorimetric target colour values, - calculating densitometric, colorimetric or spectral actual tone values or actual dot gains on the basis of the calculated colorimetric actual colour values of a subset of the colour patches that have been measured in the test element by the spectral colour measurement device and displaying them on the display device, - inputting densitometric, colorimetric or spectral target tone values or target dot gains and calculating adapted spectral colour values by comparing with the calculated colorimetric actual colour values and - saving the adapted spectral colour values to calculate colour profiles, process calibrations and process control values and controlling the printing machine on the basis of the colour profiles, process calibrations and process control values.
A stationary ring surrounding a hot gas passage of a gas turbine, said ring being surrounded by a stationary annular housing so as to co-operate therewith to define an annular cooling chamber into which there opens out at least one orifice passing through said stationary housing, said ring being made up of a plurality of ring segments, the ring being characterized in that each ring segment includes a top internal cooling circuit and a bottom internal cooling circuit (B, B'), said bottom cooling circuit (B, B') being independent of said top cooling circuit, being radially offset relative to said top cooling circuit, and including at least one cooling air feed orifice leading from the cooling chamber.