A method for preparing an oil-in-water emulsion comprising a water phase and 1 to 40%, preferably 5 to 30%, most preferably 10 to 30%, of an oil phase based on the total weight of the emulsion, wherein the method comprises the following steps: a) Providing an oil phase comprising one or more oils selected from the group consisting of fish oil, fish oil extract, krill oil, microbially produced oils, algae oils, fungal oils, and vegetable oils, and optionally a pharmaceutically acceptable antioxidant and/or at least one pharmaceutically acceptable co-emulsifier, b) Providing a water phase 1 comprising water and optionally at least one pharmaceutically acceptable co-emulsifier and/or at least one substance for adjusting the pH value and/or at least one pharmaceutically acceptable preservative and/or at least one pharmaceutically acceptable isotonic agent, wherein the isotonic agent is present in a concentration of at most 18%, based on the total weight of the first water phase, c) Preparing a pre-emulsion by mixing the oil phase provided in step a) with the water phase 1 provided in step b), d) Preparing a first emulsion by homogenizing the pre-emulsion provided in step c), e) Providing a water phase 2 comprising water and optionally at least one pharmaceutically acceptable isotonic agent and/or at least one substance for adjusting the pH value and/or at least one pharmaceutically acceptable preservative, f) Preparing the emulsion by mixing the first emulsion provided in step d) with the water phase 2 provided in step e) and g) Sterilizing the emulsion obtained in step f), wherein the emulsion is filled into a suitable container before or after sterilization, wherein in step a) and/or in step b) at least one pharmaceutically acceptable emulsifier is added, and wherein the water phase 1 provided in step b) provides not more than 30%, preferably not more than 20%, of the total amount of water contained in the emulsion. a) Providing an oil phase comprising one or more oils selected from the group consisting of fish oil, fish oil extract, krill oil, microbially produced oils, algae oils, fungal oils, and vegetable oils, and optionally a pharmaceutically acceptable antioxidant and/or at least one pharmaceutically acceptable co-emulsifier, b) Providing a water phase 1 comprising water and optionally at least one pharmaceutically acceptable co-emulsifier and/or at least one substance for adjusting the pH value and/or at least one pharmaceutically acceptable preservative and/or at least one pharmaceutically acceptable isotonic agent, wherein the isotonic agent is present in a concentration of at most 18%, based on the total weight of the first water phase, c) Preparing a pre-emulsion by mixing the oil phase provided in step a) with the water phase 1 provided in step b), d) Preparing a first emulsion by homogenizing the pre-emulsion provided in step c), e) Providing a water phase 2 comprising water and optionally at least one pharmaceutically acceptable isotonic agent and/or at least one substance for adjusting the pH value and/or at least one pharmaceutically acceptable preservative, f) Preparing the emulsion by mixing the first emulsion provided in step d) with the water phase 2 provided in step e) and g) Sterilizing the emulsion obtained in step f), wherein the emulsion is filled into a suitable container before or after sterilization, wherein in step a) and/or in step b) at least one pharmaceutically acceptable emulsifier is added, and wherein the water phase 1 provided in step b) provides not more than 30%, preferably not more than 20%, of the total amount of water contained in the emulsion.