An electroporation device adapted to supply to at least a pair of electrodes (2-a, 2-b) available in a portion of tissue of the human body a periodic voltage V out having voltage V and controllable total duration τ; the electrodes (2-a, 2-b) with elongated rectilinear shape have a section s and are arranged facing and spaced from each other by a distance d; the electroporation device comprises an electronic unit provided with a signal generator and a power amplifier which supplies the voltage V out to the electrodes (2-a, 2-b); the electroporation device is configured to perform the following operations: generate at least a first test pulse V test which is supplied to the pair of electrodes (2-a, 2-b) and therefore to the tissue T; measure the current I test which flows between the pair of electrodes (2-a, 2-b) and which depends on the tissue characteristics; detect the resistance value R A,B between the pair of electrodes according to Ohm's law as: R A, B = V test / I test store the resistance value R A,B detected; characterized by calculating, selecting and storing a pair of values of total duration τ (n,t) and voltage V that satisfy the inequality: σ ⋅ V / d 2 ⋅ τ n t ρ > AD R where: AD R represents the energy dose that realises a reversible electroporation - the energy dose AD R is a known value stored in the device; σ is defined as the inverse of resistivity σ =1/; d is the spacing of the electrodes; τ(n,t) represents the total duration of exposure to the local electric field in the case of periodic pulses that is given by the number n of pulses multiplied by the time duration t of each pulse; the resistivity = R*S/d where R denotes the measured and stored resistance value and S the section of the electrodes (2-a, 2-b); and control the power amplifier so that a voltage equal to the voltage V is applied to the electrodes and supplied to the tissue portion for the determined total duration τ(n,t). the electrodes (2-a, 2-b) with elongated rectilinear shape have a section s and are arranged facing and spaced from each other by a distance d; the electroporation device comprises an electronic unit provided with a signal generator and a power amplifier which supplies the voltage V out to the electrodes (2-a, 2-b); generate at least a first test pulse V test which is supplied to the pair of electrodes (2-a, 2-b) and therefore to the tissue T; measure the current I test which flows between the pair of electrodes (2-a, 2-b) and which depends on the tissue characteristics; detect the resistance value R A,B between the pair of electrodes according to Ohm's law as: R A, B = V test / I test store the resistance value R A,B detected; characterized by calculating, selecting and storing a pair of values of total duration τ (n,t) and voltage V that satisfy the inequality: σ ⋅ V / d 2 ⋅ τ n t ρ > AD R where: AD R represents the energy dose that realises a reversible electroporation - the energy dose AD R is a known value stored in the device; σ is defined as the inverse of resistivity σ =1/; d is the spacing of the electrodes; τ(n,t) represents the total duration of exposure to the local electric field in the case of periodic pulses that is given by the number n of pulses multiplied by the time duration t of each pulse; the resistivity = R*S/d where R denotes the measured and stored resistance value and S the section of the electrodes (2-a, 2-b); and control the power amplifier so that a voltage equal to the voltage V is applied to the electrodes and supplied to the tissue portion for the determined total duration τ(n,t). AD R represents the energy dose that realises a reversible electroporation - the energy dose AD R is a known value stored in the device; σ is defined as the inverse of resistivity σ =1/; d is the spacing of the electrodes; τ(n,t) represents the total duration of exposure to the local electric field in the case of periodic pulses that is given by the number n of pulses multiplied by the time duration t of each pulse; the resistivity = R*S/d where R denotes the measured and stored resistance value and S the section of the electrodes (2-a, 2-b); and control the power amplifier so that a voltage equal to the voltage V is applied to the electrodes and supplied to the tissue portion for the determined total duration τ(n,t).