A method for detection of the presence of a target nucleic acid sequence or detection of the presence of a variant sequence in a target nucleic acid sequence in a sample comprising the steps of a) providing a sample comprising template nucleic acids b) providing a set of primers comprising at least a pair of primers specifically capable of amplification of the target nucleic acid sequence, wherein the set of primers at least comprises a primer-H and a primer-L, wherein the melting temperature of primer-H is at least 16°C higher, such as at least 20°C higher than the melting temperature of primer-L, and wherein primer-L contains a sequence complementary to a fragment of the elongation product of primer-H, c) providing a nucleic acid polymerase having polymerase activity at an elongation temperature, d) preparing partitioned PCR reactions each comprising a part of the sample, the set of primers, the nucleic acid polymerase, PCR reagents and optionally detection reagents e) performing an asymmetric incremental polymerase reaction (AIPR) comprising the steps of: i. incubating the partitioned PCR reactions at a denaturation temperature, thereby denaturing DNA to single-stranded molecules ii. incubating the partitioned PCR reactions at a high annealing temperature allowing annealing of primer-H, but not of primer-L, iii. optionally incubating the partitioned PCR reactions at the elongation temperature, iv. optionally repeating steps i to iii, v. thereby amplifying only one strand of the target nucleic acid sequence f) performing a polymerase chain reaction comprising the steps of: 1) incubating the partitioned PCR reactions at a denaturation temperature, thereby denaturing DNA to single-stranded molecules 2) incubating the PCR at a low annealing temperature allowing annealing of both primer-H and primer-L, 3) incubating the PCR at the elongation temperature thereby allowing extension of all annealed primers 4) optionally repeating steps II to IV, 5) thereby amplifying both strands of the target nucleic acid sequence to obtain a PCR product g) detecting whether the PCR product comprises the target nucleic acid sequence or the variant sequence in the target nucleic acid sequence. a) providing a sample comprising template nucleic acids b) providing a set of primers comprising at least a pair of primers specifically capable of amplification of the target nucleic acid sequence, wherein the set of primers at least comprises a primer-H and a primer-L, wherein the melting temperature of primer-H is at least 16°C higher, such as at least 20°C higher than the melting temperature of primer-L, and wherein primer-L contains a sequence complementary to a fragment of the elongation product of primer-H, c) providing a nucleic acid polymerase having polymerase activity at an elongation temperature, d) preparing partitioned PCR reactions each comprising a part of the sample, the set of primers, the nucleic acid polymerase, PCR reagents and optionally detection reagents e) performing an asymmetric incremental polymerase reaction (AIPR) comprising the steps of: i. incubating the partitioned PCR reactions at a denaturation temperature, thereby denaturing DNA to single-stranded molecules ii. incubating the partitioned PCR reactions at a high annealing temperature allowing annealing of primer-H, but not of primer-L, iii. optionally incubating the partitioned PCR reactions at the elongation temperature, iv. optionally repeating steps i to iii, v. thereby amplifying only one strand of the target nucleic acid sequence i. incubating the partitioned PCR reactions at a denaturation temperature, thereby denaturing DNA to single-stranded molecules ii. incubating the partitioned PCR reactions at a high annealing temperature allowing annealing of primer-H, but not of primer-L, iii. optionally incubating the partitioned PCR reactions at the elongation temperature, iv. optionally repeating steps i to iii, v. thereby amplifying only one strand of the target nucleic acid sequence f) performing a polymerase chain reaction comprising the steps of: 1) incubating the partitioned PCR reactions at a denaturation temperature, thereby denaturing DNA to single-stranded molecules 2) incubating the PCR at a low annealing temperature allowing annealing of both primer-H and primer-L, 3) incubating the PCR at the elongation temperature thereby allowing extension of all annealed primers 4) optionally repeating steps II to IV, 5) thereby amplifying both strands of the target nucleic acid sequence to obtain a PCR product 1) incubating the partitioned PCR reactions at a denaturation temperature, thereby denaturing DNA to single-stranded molecules 2) incubating the PCR at a low annealing temperature allowing annealing of both primer-H and primer-L, 3) incubating the PCR at the elongation temperature thereby allowing extension of all annealed primers 4) optionally repeating steps II to IV, 5) thereby amplifying both strands of the target nucleic acid sequence to obtain a PCR product g) detecting whether the PCR product comprises the target nucleic acid sequence or the variant sequence in the target nucleic acid sequence.