A method of detecting a target non-nucleic acid molecule and a target nucleic acid molecule in a sample, comprising the steps of: a) contacting a sample comprising a target non-nucleic acid molecule and a target nucleic acid molecule with a binding agent under conditions that permit binding between the binding agent and the target non-nucleic acid molecule in the sample, wherein the binding agent is covalently conjugated to an oligonucleotide that comprises a binding agent identification tag and a poly sequence; b) reverse transcribing the conjugated oligonucleotide and the target nucleic acid molecule in the sample simultaneously, thereby producing a conjugated oligonucleotide reverse transcription product and a target nucleic acid molecule reverse transcription product, wherein reverse transcription of the conjugated oligonucleotide is primed using an extension primer that comprises a poly(dT) sequence, wherein the length of the poly(dT) sequence is greater than or equal to the length of the poly sequence in the conjugated oligonucleotide; c) amplifying the conjugated oligonucleotide reverse transcription product and the target nucleic acid molecule reverse transcription product simultaneously, thereby producing a conjugated oligonucleotide amplicon and a target nucleic acid molecule amplicon; and d) detecting: 1) the conjugated oligonucleotide amplicon, thereby detecting the target non-nucleic acid molecule in the sample; and 2) the target nucleic acid molecule amplicon, thereby detecting the target nucleic molecule in the sample. a) contacting a sample comprising a target non-nucleic acid molecule and a target nucleic acid molecule with a binding agent under conditions that permit binding between the binding agent and the target non-nucleic acid molecule in the sample, wherein the binding agent is covalently conjugated to an oligonucleotide that comprises a binding agent identification tag and a poly sequence; b) reverse transcribing the conjugated oligonucleotide and the target nucleic acid molecule in the sample simultaneously, thereby producing a conjugated oligonucleotide reverse transcription product and a target nucleic acid molecule reverse transcription product, wherein reverse transcription of the conjugated oligonucleotide is primed using an extension primer that comprises a poly(dT) sequence, wherein the length of the poly(dT) sequence is greater than or equal to the length of the poly sequence in the conjugated oligonucleotide; c) amplifying the conjugated oligonucleotide reverse transcription product and the target nucleic acid molecule reverse transcription product simultaneously, thereby producing a conjugated oligonucleotide amplicon and a target nucleic acid molecule amplicon; and d) detecting: 1) the conjugated oligonucleotide amplicon, thereby detecting the target non-nucleic acid molecule in the sample; and 2) the target nucleic acid molecule amplicon, thereby detecting the target nucleic molecule in the sample. 1) the conjugated oligonucleotide amplicon, thereby detecting the target non-nucleic acid molecule in the sample; and 2) the target nucleic acid molecule amplicon, thereby detecting the target nucleic molecule in the sample.