A reaction device, comprising: a rotating disk configured to incubate, wash, and measure a reactant mixed with a sample in a reactor; a washing assembly located above the rotating disk, and capable of dispensing a washing solution into the reactor and drawing unbound ingredients in the reactor, and the washing assembly comprising a signal reagent dispensing portion configured to dispense a signal reagent into the reactor subjected to a BF operation; a measuring device connected to the rotating disk, and configured to measure an optical signal of the reactant in the reactor after adding the signal reagent; and a transferring assembly configured to transfer the reactor; wherein the rotating disk comprises an incubation ring, a washing ring, and a measuring ring that are arranged around a rotation center thereof, the incubation ring is provided with incubation positions that are arranged at intervals along a circumferential direction thereof and configured to incubate the reactant in the reactor, the washing ring is provided with washing positions that are arranged at intervals along a circumferential direction thereof and configured to wash the reactant in the reactor, the measuring ring is provided with measuring positions that are arranged at intervals along a circumferential direction thereof and configured to measure a signal of the reactant in the reactor, characterized in that the device further comprises: - a signal reagent mixing unit configured to mix the signal reagent in the reactor after adding the signal reagent; and wherein the transferring assembly is configured to transfer the reactor between the rotating disc and the signal reagent mixing unit, and to transfer the reactor between the signal reagent mixing unit, the incubation position, the washing position, and the measuring position, wherein a moving trajectory of the transferring assembly is on the same straight line. a rotating disk configured to incubate, wash, and measure a reactant mixed with a sample in a reactor; a washing assembly located above the rotating disk, and capable of dispensing a washing solution into the reactor and drawing unbound ingredients in the reactor, and the washing assembly comprising a signal reagent dispensing portion configured to dispense a signal reagent into the reactor subjected to a BF operation; a measuring device connected to the rotating disk, and configured to measure an optical signal of the reactant in the reactor after adding the signal reagent; and a transferring assembly configured to transfer the reactor; wherein the rotating disk comprises an incubation ring, a washing ring, and a measuring ring that are arranged around a rotation center thereof, the incubation ring is provided with incubation positions that are arranged at intervals along a circumferential direction thereof and configured to incubate the reactant in the reactor, the washing ring is provided with washing positions that are arranged at intervals along a circumferential direction thereof and configured to wash the reactant in the reactor, the measuring ring is provided with measuring positions that are arranged at intervals along a circumferential direction thereof and configured to measure a signal of the reactant in the reactor, characterized in that the device further comprises: - a signal reagent mixing unit configured to mix the signal reagent in the reactor after adding the signal reagent; - a signal reagent mixing unit configured to mix the signal reagent in the reactor after adding the signal reagent; and wherein the transferring assembly is configured to transfer the reactor between the rotating disc and the signal reagent mixing unit, and to transfer the reactor between the signal reagent mixing unit, the incubation position, the washing position, and the measuring position, wherein a moving trajectory of the transferring assembly is on the same straight line.