Tubulysin compounds having the general formula wherein: W is a group selected from OR 13 or NR 14 R 15, X is a substituted five- or six-membered aromatic (arylene) or heteroaromatic ring (hetero-arylene), Y is H, -CH 2 -, NH, NMe, a sulfur atom or oxygen atom, Z is a group selected from OR 16, NR 17 R 18 or: R 1, R 2, R 3, R 4, R 5, R 6, R 7, R 8, R 9, R 10, R 11, R 13, R 14, R 15, R 16, R 17, R 18, R 20, R 21, R 23, R 24 and R 25 each independently represents H, a substituted or unsubstituted alkyl, a substituted or unsubstituted cycloalkyl, a substituted or unsubstituted aryl, a substituted or unsubstituted heteroaryl, a substituted or unsubstituted heteroalkyl, and where R 1 and R 3 or R 1 and R 2 may be linked to form together with the nitrogen atom a five-membered pyrrolidine ring or a six-membered piperidine ring, R 12 is H, an acyl group (C R), a substituted or unsubstituted alkyl, a substituted or unsubstituted cycloalkyl, a substituted or unsubstituted aryl, with R being a substituted or unsubstituted alkyl, a substituted or unsubstituted cycloalkyl, or a substituted or unsubstituted aryl, R 19 represents H, halogen, nitro, amine, monoalkylamine or dialkylamine group, hydroxyl, alkyloxy, a substituted or unsubstituted alkyl, a substituted or unsubstituted cycloalkyl, a substituted or unsubstituted aryl, a substituted or unsubstituted heteroaryl, a substituted or unsubstituted heteroalkyl; in positions 2 and/or 3 and/or 4, and/or 5, and/or 6, or in any combination, R 22 represents OH, NH 2, alkyloxy, alkylamino or dialkylamino group, or a linker, V is a sulfur or oxygen atom, -CH 2 -, NH or NH-alkyl group, and pharmaceutical acceptable salts, hydrates, solvates, stereoisomers, stereoisomeric mixtures, and polymorphs thereof. W is a group selected from OR 13 or NR 14 R 15, X is a substituted five- or six-membered aromatic (arylene) or heteroaromatic ring (hetero-arylene), Y is H, -CH 2 -, NH, NMe, a sulfur atom or oxygen atom, Z is a group selected from OR 16, NR 17 R 18 or: R 1, R 2, R 3, R 4, R 5, R 6, R 7, R 8, R 9, R 10, R 11, R 13, R 14, R 15, R 16, R 17, R 18, R 20, R 21, R 23, R 24 and R 25 each independently represents H, a substituted or unsubstituted alkyl, a substituted or unsubstituted cycloalkyl, a substituted or unsubstituted aryl, a substituted or unsubstituted heteroaryl, a substituted or unsubstituted heteroalkyl, and where R 1 and R 3 or R 1 and R 2 may be linked to form together with the nitrogen atom a five-membered pyrrolidine ring or a six-membered piperidine ring, R 12 is H, an acyl group (C R), a substituted or unsubstituted alkyl, a substituted or unsubstituted cycloalkyl, a substituted or unsubstituted aryl, with R being a substituted or unsubstituted alkyl, a substituted or unsubstituted cycloalkyl, or a substituted or unsubstituted aryl, R 19 represents H, halogen, nitro, amine, monoalkylamine or dialkylamine group, hydroxyl, alkyloxy, a substituted or unsubstituted alkyl, a substituted or unsubstituted cycloalkyl, a substituted or unsubstituted aryl, a substituted or unsubstituted heteroaryl, a substituted or unsubstituted heteroalkyl; in positions 2 and/or 3 and/or 4, and/or 5, and/or 6, or in any combination, R 22 represents OH, NH 2, alkyloxy, alkylamino or dialkylamino group, or a linker, V is a sulfur or oxygen atom, -CH 2 -, NH or NH-alkyl group,