A mutant glucose oxidase modified at one or more positions selected from: (a) a position corresponding to the position 53 of the amino acid sequence set forth in SEQ ID NO: 1 by substituting the amino acid residue Thr with Ser; (b) a position corresponding to the position 116 of the amino acid sequence set forth in SEQ ID NO: 1 by substituting the amino acid residue Ile with Val; (c) a position corresponding to the position 132 of the amino acid sequence set forth in SEQ ID NO: 1 by substituting the amino acid residue Ser with Ala, Thr, Val, Cys or Ile, or by substituting the amino acid residue Thr with Ala, Ser, Val, Trp or Cys; (d) a position corresponding to the position 134 of the amino acid sequence set forth in SEQ ID NO: 1 by substituting the amino acid residue Thr with Ala, Ile or Met; (e) a position corresponding to the position 237 of the amino acid sequence set forth in SEQ ID NO: 1 by substituting the amino acid residue Ile with Val, or by substituting the amino acid residue Phe with Ile, Ala, Val, Met, Ser, Asp, Leu, Thr, Asn, Arg or Cys; (f) a position corresponding to the position 371 of the amino acid sequence set forth in SEQ ID NO: 1 by substituting the amino acid residue Val with Thr, Ala, or by substituting the amino acid residue Ala with Val; (g) a position corresponding to the position 373 of the amino acid sequence set forth in SEQ ID NO: 1 by substituting the amino acid residue Phe with Leu, Tyr, Ala, Met, Asn or Trp; (h) a position corresponding to the position 434 of the amino acid sequence set forth in SEQ ID NO: 1 by substituting the amino acid residue Glu with Gln; (i) a position corresponding to the position 436 of the amino acid sequence set forth in SEQ ID NO: 1 by substituting the amino acid residue Phe with Trp, Ala, Leu, Tyr, Met, Glu or Ile; (j) a position corresponding to the position 448 of the amino acid sequence set forth in SEQ ID NO: 1 by substituting the amino acid residue Trp with Ala, Ile, Ser, Val, Met, Thr, Cys, Gly, Leu, Asn, Asp, Lys, Phe, Gln or Tyr; and (k) a position corresponding to the position 537 of the amino acid sequence set forth in SEQ ID NO: 1 by substituting the amino acid residue Trp with Ala. (a) a position corresponding to the position 53 of the amino acid sequence set forth in SEQ ID NO: 1 by substituting the amino acid residue Thr with Ser; (b) a position corresponding to the position 116 of the amino acid sequence set forth in SEQ ID NO: 1 by substituting the amino acid residue Ile with Val; (c) a position corresponding to the position 132 of the amino acid sequence set forth in SEQ ID NO: 1 by substituting the amino acid residue Ser with Ala, Thr, Val, Cys or Ile, or by substituting the amino acid residue Thr with Ala, Ser, Val, Trp or Cys; (d) a position corresponding to the position 134 of the amino acid sequence set forth in SEQ ID NO: 1 by substituting the amino acid residue Thr with Ala, Ile or Met; (e) a position corresponding to the position 237 of the amino acid sequence set forth in SEQ ID NO: 1 by substituting the amino acid residue Ile with Val, or by substituting the amino acid residue Phe with Ile, Ala, Val, Met, Ser, Asp, Leu, Thr, Asn, Arg or Cys; (f) a position corresponding to the position 371 of the amino acid sequence set forth in SEQ ID NO: 1 by substituting the amino acid residue Val with Thr, Ala, or by substituting the amino acid residue Ala with Val; (g) a position corresponding to the position 373 of the amino acid sequence set forth in SEQ ID NO: 1 by substituting the amino acid residue Phe with Leu, Tyr, Ala, Met, Asn or Trp; (h) a position corresponding to the position 434 of the amino acid sequence set forth in SEQ ID NO: 1 by substituting the amino acid residue Glu with Gln; (i) a position corresponding to the position 436 of the amino acid sequence set forth in SEQ ID NO: 1 by substituting the amino acid residue Phe with Trp, Ala, Leu, Tyr, Met, Glu or Ile; (j) a position corresponding to the position 448 of the amino acid sequence set forth in SEQ ID NO: 1 by substituting the amino acid residue Trp with Ala, Ile, Ser, Val, Met, Thr, Cys, Gly, Leu, Asn, Asp, Lys, Phe, Gln or Tyr; and (k) a position corresponding to the position 537 of the amino acid sequence set forth in SEQ ID NO: 1 by substituting the amino acid residue Trp with Ala.