A centrifugal micro-fluidic device comprising: at least one micro-fluidic structure comprising a chamber and at least one channel connected with the chamber; and a detection unit, wherein the chamber comprises a reaction chamber comprising a detectable signal generator for combining the detectable signal generator with an analyte of a liquid specimen to create a detectable signal generator-analyte complex, and an analysis chamber located downstream from the reaction chamber, wherein the detectable signal generator is in a liquid or solid phase, wherein the analysis chamber comprises a holder (410, 410') which carries a fluid provided from the reaction chamber and a detection region (420, 420') for combining a capture binder with the detectable signal generator analyte complex, wherein the detection region (420, 420') includes one of porous membranes, micro-pore structures and micro-pillars, wherein the detection region (420, 420') comprises a test region (430, 430') to which the capture binder is fixed, and a control region (440, 440') which is located downstream of the test region (430, 430') relative to a direction A of capillary action and separated from the test region (430, 430') by a distance, and wherein the detection region (420, 420') is arranged such that application of a capillary force moves fluid along the detection region (420, 420'), completely shifting the fluid to the test region (430, 430') and the control region (440, 440'), and application of a centrifugal force delivers the fluid back into the holder (410, 410'), wherein the centrifugal force has a direction B opposite to the direction A of capillary action. at least one micro-fluidic structure comprising a chamber and at least one channel connected with the chamber; and a detection unit, wherein the chamber comprises a reaction chamber comprising a detectable signal generator for combining the detectable signal generator with an analyte of a liquid specimen to create a detectable signal generator-analyte complex, and an analysis chamber located downstream from the reaction chamber, wherein the detectable signal generator is in a liquid or solid phase, wherein the analysis chamber comprises a holder (410, 410') which carries a fluid provided from the reaction chamber and a detection region (420, 420') for combining a capture binder with the detectable signal generator analyte complex, wherein the detection region (420, 420') includes one of porous membranes, micro-pore structures and micro-pillars, wherein the detection region (420, 420') comprises a test region (430, 430') to which the capture binder is fixed, and a control region (440, 440') which is located downstream of the test region (430, 430') relative to a direction A of capillary action and separated from the test region (430, 430') by a distance, and wherein the detection region (420, 420') is arranged such that application of a capillary force moves fluid along the detection region (420, 420'), completely shifting the fluid to the test region (430, 430') and the control region (440, 440'), and application of a centrifugal force delivers the fluid back into the holder (410, 410'), wherein the centrifugal force has a direction B opposite to the direction A of capillary action.