A biochemical cartridge and cartridge holder set comprising: a cartridge for sending a liquid inside a sealed space provided within the cartridge, the cartridge comprising: a cartridge main body; and a plurality of chambers (6 - 10) internally provided in the cartridge main body, each of the chambers (6 - 10) having an opening and being configured to accept the liquid; characterized by further comprising a membrane attached to a bottom surface of the cartridge main body, and that is configured to undergo deformation with changes in externally applied pressure, the membrane being functionalized to close the openings at the bottom surface side of the plurality of chambers (6 - 10), and to form a channel (14, 15) between the plurality of chambers (6 - 10), wherein said membrane is not bonded to the portion of the cartridge body that becomes the channel (14, 15) between the chambers (6 - 10) and a cartridge holder on which the biochemical cartridge is set, wherein the cartridge holder has a holder top with a channel groove formed on it such that a channel shape is formed therein, wherein each channel groove located between chambers comprises a channel pressurization port (68-a; 68-b) and a channel depressurization port (69-a, 69-b), wherein a channel is formed in the main body of the biochemical cartridge in conformity with the channel shape set upon application of negative pressure from outside using said channel depressurization port of the channel groove in the cartridge holder so as to deform the membrane downward to bring it into contact with the channel groove of the cartridge holder top portion, wherein plungers (64-a - 64-d) are provided in a portion of the cartridge holder immediately below each chamber internally, said plungers (64-a - 64-d) being configured to close said openings of the chambers by deformation of the membrane, and wherein the liquid is sent to the channel (14, 15) and is further sent to a downstream side - when the membrane is allowed to contact the holder top upon application of negative pressure by said channel depressurization port (69-a, 69-b) while the opening to the downstream chamber is closed, using the pertinent plunger located in the cartridge holder and - when the membrane is allowed to contact the cartridge main body upon application of positive pressure by said channel pressurization port (68-a, 68b) provided in the cartridge holder together with closing the opening of the chamber located upstream of the channel using the pertinent plunger (64-a - 64-d) located in the cartridge holder. a cartridge main body; and a plurality of chambers (6 - 10) internally provided in the cartridge main body, each of the chambers (6 - 10) having an opening and being configured to accept the liquid; characterized by further comprising a membrane attached to a bottom surface of the cartridge main body, and that is configured to undergo deformation with changes in externally applied pressure, the membrane being functionalized to close the openings at the bottom surface side of the plurality of chambers (6 - 10), and to form a channel (14, 15) between the plurality of chambers (6 - 10), wherein said membrane is not bonded to the portion of the cartridge body that becomes the channel (14, 15) between the chambers (6 - 10) and a cartridge holder on which the biochemical cartridge is set, wherein the cartridge holder has a holder top with a channel groove formed on it such that a channel shape is formed therein, wherein each channel groove located between chambers comprises a channel pressurization port (68-a; 68-b) and a channel depressurization port (69-a, 69-b), wherein a channel is formed in the main body of the biochemical cartridge in conformity with the channel shape set upon application of negative pressure from outside using said channel depressurization port of the channel groove in the cartridge holder so as to deform the membrane downward to bring it into contact with the channel groove of the cartridge holder top portion, wherein plungers (64-a - 64-d) are provided in a portion of the cartridge holder immediately below each chamber internally, said plungers (64-a - 64-d) being configured to close said openings of the chambers by deformation of the membrane, and wherein the liquid is sent to the channel (14, 15) and is further sent to a downstream side - when the membrane is allowed to contact the holder top upon application of negative pressure by said channel depressurization port (69-a, 69-b) while the opening to the downstream chamber is closed, using the pertinent plunger located in the cartridge holder and - when the membrane is allowed to contact the cartridge main body upon application of positive pressure by said channel pressurization port (68-a, 68b) provided in the cartridge holder together with closing the opening of the chamber located upstream of the channel using the pertinent plunger (64-a - 64-d) located in the cartridge holder. - when the membrane is allowed to contact the holder top upon application of negative pressure by said channel depressurization port (69-a, 69-b) while the opening to the downstream chamber is closed, using the pertinent plunger located in the cartridge holder and - when the membrane is allowed to contact the cartridge main body upon application of positive pressure by said channel pressurization port (68-a, 68b) provided in the cartridge holder together with closing the opening of the chamber located upstream of the channel using the pertinent plunger (64-a - 64-d) located in the cartridge holder.