A method for purifying a polypeptide from a composition comprising the polypeptide and one or more impurities, wherein the polypeptide is produced in a prokaryotic cell, and wherein the cell is engineered to express one or more chaperones, wherein the chaperone is one or more of FkpA, DsbA or DsbC and wherein the polypeptide is a multispecific antibody, wherein the multispecific antibody comprises i) multiple arms, each arm comprising a VH/VL unit, the method comprising the sequential steps of a) subjecting the composition to protein A chromatography to produce a protein A eluate, b) subjecting the protein A eluate to mixed-mode chromatography to generate a mixed-mode eluate, and c) subjecting the mixed-mode eluate to anion exchange chromatography to generate an anion exchange eluate, d) subjecting the anion exchange eluate to hydrophobic interaction chromatography and collecting a fraction comprising the multispecific antibody, e) subjecting the hydrophobic interaction eluate to cation exchange chromatography and collecting a fraction comprising the polypeptide, wherein the method reduces the amount of FkpA, DsbA and/or DsbC from the composition; or ii) multiple arms, each arm comprising a VH/VL unit, wherein each arm of the multispecific antibody is produced separately, the method comprising the sequential steps of a) subjecting each arm of the multispecific antibody to protein A chromatography to produce protein A eluates for each arm of the multispecific antibody, b) forming a mixture comprising protein A eluates of each arm of the multispecific antibody under conditions sufficient to produce a composition comprising the multispecific antibody c) subjecting the composition comprising the multispecific antibody to mixed-mode chromatography to generate a mixed-mode eluate, and d) subjecting the mixed-mode eluate to anion exchange chromatography to generate an anion exchange eluate, e) subjecting the anion exchange eluate to hydrophobic interaction chromatography and collecting a fraction comprising the multispecific antibody, f) subjecting the hydrophobic interaction eluate to cation exchange chromatography and collecting a fraction comprising the polypeptide, wherein the method reduces the amount of FkpA, DsbA and/or DsbC from the composition. i) multiple arms, each arm comprising a VH/VL unit, the method comprising the sequential steps of a) subjecting the composition to protein A chromatography to produce a protein A eluate, b) subjecting the protein A eluate to mixed-mode chromatography to generate a mixed-mode eluate, and c) subjecting the mixed-mode eluate to anion exchange chromatography to generate an anion exchange eluate, d) subjecting the anion exchange eluate to hydrophobic interaction chromatography and collecting a fraction comprising the multispecific antibody, e) subjecting the hydrophobic interaction eluate to cation exchange chromatography and collecting a fraction comprising the polypeptide, wherein the method reduces the amount of FkpA, DsbA and/or DsbC from the composition; or a) subjecting the composition to protein A chromatography to produce a protein A eluate, b) subjecting the protein A eluate to mixed-mode chromatography to generate a mixed-mode eluate, and c) subjecting the mixed-mode eluate to anion exchange chromatography to generate an anion exchange eluate, d) subjecting the anion exchange eluate to hydrophobic interaction chromatography and collecting a fraction comprising the multispecific antibody, e) subjecting the hydrophobic interaction eluate to cation exchange chromatography and collecting a fraction comprising the polypeptide, ii) multiple arms, each arm comprising a VH/VL unit, wherein each arm of the multispecific antibody is produced separately, the method comprising the sequential steps of a) subjecting each arm of the multispecific antibody to protein A chromatography to produce protein A eluates for each arm of the multispecific antibody, b) forming a mixture comprising protein A eluates of each arm of the multispecific antibody under conditions sufficient to produce a composition comprising the multispecific antibody c) subjecting the composition comprising the multispecific antibody to mixed-mode chromatography to generate a mixed-mode eluate, and d) subjecting the mixed-mode eluate to anion exchange chromatography to generate an anion exchange eluate, e) subjecting the anion exchange eluate to hydrophobic interaction chromatography and collecting a fraction comprising the multispecific antibody, f) subjecting the hydrophobic interaction eluate to cation exchange chromatography and collecting a fraction comprising the polypeptide, wherein the method reduces the amount of FkpA, DsbA and/or DsbC from the composition. a) subjecting each arm of the multispecific antibody to protein A chromatography to produce protein A eluates for each arm of the multispecific antibody, b) forming a mixture comprising protein A eluates of each arm of the multispecific antibody under conditions sufficient to produce a composition comprising the multispecific antibody c) subjecting the composition comprising the multispecific antibody to mixed-mode chromatography to generate a mixed-mode eluate, and d) subjecting the mixed-mode eluate to anion exchange chromatography to generate an anion exchange eluate, e) subjecting the anion exchange eluate to hydrophobic interaction chromatography and collecting a fraction comprising the multispecific antibody, f) subjecting the hydrophobic interaction eluate to cation exchange chromatography and collecting a fraction comprising the polypeptide,