A suction puncture device comprising: a puncture needle having an outer tube (11, 122, 123) that has an opening edge of a front end formed in the shape of a blade, and a stopper member (12, 101, 102, 103, 104, 131, 132, 133, 134) that has a front end disposed within the outer tube (11, 122, 123) at a position separated from the front end of the outer tube (11, 122, 123) by a predetermined distance; a sample containing portion formed between the front end of the stopper member (12, 101, 102, 103, 104, 131, 132, 133, 134) and the front end of the outer tube (11, 122, 123); a ventilation passage formed around the stopper member (12, 101, 102, 103, 104, 131, 132, 133, 134) within the outer tube (11, 122, 123); and a suction unit connected to a base end side of the outer tube (11, 122, 123), and communicated with the sample containing portion via the ventilation passage, wherein the outer tube (11, 122, 123) has a connecting portion including a stopper member insertion port (17, 111, 112, 113, 114) which allows the stopper member (12, 101, 102, 103, 104, 131, 132, 133, 134) to be airtightly inserted thereinto and a suction unit communication port which allows the suction unit to be airtightly connected thereto on the base end side; the stopper member (12, 101, 102, 103, 104, 131, 132, 133, 134) has a larger-diameter portion of which the radial dimension is enlarged, at a portion thereof in the longitudinal direction; and an annular elastic body is arranged in the stopper member insertion port (17, 111, 112, 113, 114) so as to allow the stopper member (12, 101, 102, 103, 104, 131, 132, 133, 134) to be inserted through the insertion port (17, 111, 112, 113, 114); characterized in that the sample containing portion is formed by retracting the stopper member (12, 101, 102, 103, 104, 131, 132, 133, 134) from a state where the larger-diameter portion is located outside a hole of the elastic body to a state where the larger-diameter portion is located within the hole of the elastic body and the stopper member insertion port (17, 111, 112, 113, 114) is airtightly closed in a state where the larger-diameter portion is located within a hole of the elastic body. a puncture needle having an outer tube (11, 122, 123) that has an opening edge of a front end formed in the shape of a blade, and a stopper member (12, 101, 102, 103, 104, 131, 132, 133, 134) that has a front end disposed within the outer tube (11, 122, 123) at a position separated from the front end of the outer tube (11, 122, 123) by a predetermined distance; a sample containing portion formed between the front end of the stopper member (12, 101, 102, 103, 104, 131, 132, 133, 134) and the front end of the outer tube (11, 122, 123); a ventilation passage formed around the stopper member (12, 101, 102, 103, 104, 131, 132, 133, 134) within the outer tube (11, 122, 123); and a suction unit connected to a base end side of the outer tube (11, 122, 123), and communicated with the sample containing portion via the ventilation passage, wherein the outer tube (11, 122, 123) has a connecting portion including a stopper member insertion port (17, 111, 112, 113, 114) which allows the stopper member (12, 101, 102, 103, 104, 131, 132, 133, 134) to be airtightly inserted thereinto and a suction unit communication port which allows the suction unit to be airtightly connected thereto on the base end side; the stopper member (12, 101, 102, 103, 104, 131, 132, 133, 134) has a larger-diameter portion of which the radial dimension is enlarged, at a portion thereof in the longitudinal direction; and an annular elastic body is arranged in the stopper member insertion port (17, 111, 112, 113, 114) so as to allow the stopper member (12, 101, 102, 103, 104, 131, 132, 133, 134) to be inserted through the insertion port (17, 111, 112, 113, 114); characterized in that the sample containing portion is formed by retracting the stopper member (12, 101, 102, 103, 104, 131, 132, 133, 134) from a state where the larger-diameter portion is located outside a hole of the elastic body to a state where the larger-diameter portion is located within the hole of the elastic body and the stopper member insertion port (17, 111, 112, 113, 114) is airtightly closed in a state where the larger-diameter portion is located within a hole of the elastic body.