An anti-pollution test device for rapid test of nucleic acid amplifications, comprising a test portion and a mixing reaction chamber, wherein the mixing reaction chamber comprises: a reaction chamber body, a hybridization solution accommodating portion and a mixing reaction portion being arranged in the reaction chamber body, and the hybridization solution accommodating portion and the mixing reaction portion being provided with chambers for accommodating a hybridization solution and a sample to be tested respectively; and a reaction chamber cover, the reaction chamber cover being hermetically connected to and detachable from the reaction chamber body, a channel in communication with the hybridization solution accommodating portion and the mixing reaction portion being arranged between the reaction chamber cover and the reaction chamber body, the hybridization solution and the sample are subjected to a mixing reaction by means of the channel when the mixing reaction chamber containing the hybridization solution and the sample in an isolated manner is inversely placed, a flow guide port penetrating the reaction chamber cover being further provided on the reaction chamber cover, and a puncturable sealing cap being arranged on the flow guide port; and the test portion comprises a test inner core and a test kit accommodating the test inner core, a transparent observation window being arranged on a position corresponding to a reaction area of the test inner core on the test kit, a flow guide device matching the mixing reaction chamber being further arranged on the test kit, and the flow guide device comprising: a flow guide tube for puncturing the sealing cap and guiding a mixed solution of the hybridization solution and the sample after the mixing reaction onto a flow guide component; and the flow guide component, one end of the flow guide component being connected to the flow guide tube, one end of the flow guide component being connected to the test inner core, to guide the mixed solution of the hybridization solution and the sample to the test inner core, and the test inner core displaying a final test result in an area corresponding to the transparent observation window. a reaction chamber body, a hybridization solution accommodating portion and a mixing reaction portion being arranged in the reaction chamber body, and the hybridization solution accommodating portion and the mixing reaction portion being provided with chambers for accommodating a hybridization solution and a sample to be tested respectively; and a reaction chamber cover, the reaction chamber cover being hermetically connected to and detachable from the reaction chamber body, a channel in communication with the hybridization solution accommodating portion and the mixing reaction portion being arranged between the reaction chamber cover and the reaction chamber body, the hybridization solution and the sample are subjected to a mixing reaction by means of the channel when the mixing reaction chamber containing the hybridization solution and the sample in an isolated manner is inversely placed, a flow guide port penetrating the reaction chamber cover being further provided on the reaction chamber cover, and a puncturable sealing cap being arranged on the flow guide port; and the test portion comprises a test inner core and a test kit accommodating the test inner core, a transparent observation window being arranged on a position corresponding to a reaction area of the test inner core on the test kit, a flow guide device matching the mixing reaction chamber being further arranged on the test kit, and the flow guide device comprising: a flow guide tube for puncturing the sealing cap and guiding a mixed solution of the hybridization solution and the sample after the mixing reaction onto a flow guide component; and the flow guide component, one end of the flow guide component being connected to the flow guide tube, one end of the flow guide component being connected to the test inner core, to guide the mixed solution of the hybridization solution and the sample to the test inner core, and the test inner core displaying a final test result in an area corresponding to the transparent observation window. a flow guide tube for puncturing the sealing cap and guiding a mixed solution of the hybridization solution and the sample after the mixing reaction onto a flow guide component; and the flow guide component, one end of the flow guide component being connected to the flow guide tube, one end of the flow guide component being connected to the test inner core, to guide the mixed solution of the hybridization solution and the sample to the test inner core, and the test inner core displaying a final test result in an area corresponding to the transparent observation window.