A method for detecting a target nucleic acid sequence from a DNA or a mixture of nucleic acids by a cyclic exonucleolytic reaction by the use of a microarray of probes on a solid phase, which comprises the steps of: (a) hybridizing the target nucleic acid sequence with a microarray of immobilized probes having a nucleotide sequence complementary to the target nucleic acid sequence and each having a single label generating a detectable signal; wherein the immobilized probes are immobilized on a solid substrate through its 3'-end; wherein the single label is located at 5'-end of the immobilized probe; wherein the immobilized probes are not target discriminative probes (TD probe) having a modified dual specificity oligonucleotide (mDSO) structure represented by the following general formula I: 5'-X' p -Y' q -Z' r -3' wherein, X' p represents a 5'-second hybridization portion having a hybridizing nucleotide sequence complementary to the target nucleic acid sequence; Y' q represents a separation portion comprising at least three universal bases, Z' r represents a 3'-first hybridization portion having a hybridizing nucleotide sequence complementary to the target nucleic acid sequence; p, q and r represent the number of nucleotides; and X', Y' and Z' are deoxyribonucleotides or ribonucleotides; the Tm of the 5'-second hybridization portion is lower than that of the 3'-first hybridization portion and the separation portion has the lowest Tm in the three portions of X' p, Y' q and Z' r; the separation portion separates the 5 - second hybridization portion from the 3'-first hybridization portion in terms of hybridization events to the target nucleic acid sequence; (b) contacting the resultant of step (a) to a thermostable enzyme having a 5' to 3' exonuclease activity under conditions for cleavage of the immobilized probe; wherein the immobilized probe is digested by the thermostable enzyme having the 5' to 3' exonuclease activity to release the single label from the immobilized probe, resulting in decrease in a signal on the solid substrate; wherein the thermostable enzyme having the 5' to 3' exonuclease activity is a template-dependent nucleic acid polymerase having a 5' to 3'exonuclease activity; (c) denaturing the resultant of step (b); (d) repeating the steps (a)-(c) at least twice to further decrease the signal on the solid substrate with no amplification of target nucleic acid sequence; and (e) detecting the signal decrease on the solid substrate, such that the signal decrease by the cleavage of the immobilized probe is indicative of the presence of the target nucleic acid sequence. (a) hybridizing the target nucleic acid sequence with a microarray of immobilized probes having a nucleotide sequence complementary to the target nucleic acid sequence and each having a single label generating a detectable signal; wherein the immobilized probes are immobilized on a solid substrate through its 3'-end; wherein the single label is located at 5'-end of the immobilized probe; wherein the immobilized probes are not target discriminative probes (TD probe) having a modified dual specificity oligonucleotide (mDSO) structure represented by the following general formula I: 5'-X' p -Y' q -Z' r -3' wherein, X' p represents a 5'-second hybridization portion having a hybridizing nucleotide sequence complementary to the target nucleic acid sequence; Y' q represents a separation portion comprising at least three universal bases, Z' r represents a 3'-first hybridization portion having a hybridizing nucleotide sequence complementary to the target nucleic acid sequence; p, q and r represent the number of nucleotides; and X', Y' and Z' are deoxyribonucleotides or ribonucleotides; the Tm of the 5'-second hybridization portion is lower than that of the 3'-first hybridization portion and the separation portion has the lowest Tm in the three portions of X' p, Y' q and Z' r; the separation portion separates the 5 - second hybridization portion from the 3'-first hybridization portion in terms of hybridization events to the target nucleic acid sequence; (b) contacting the resultant of step (a) to a thermostable enzyme having a 5' to 3' exonuclease activity under conditions for cleavage of the immobilized probe; wherein the immobilized probe is digested by the thermostable enzyme having the 5' to 3' exonuclease activity to release the single label from the immobilized probe, resulting in decrease in a signal on the solid substrate; wherein the thermostable enzyme having the 5' to 3' exonuclease activity is a template-dependent nucleic acid polymerase having a 5' to 3'exonuclease activity; (c) denaturing the resultant of step (b); (d) repeating the steps (a)-(c) at least twice to further decrease the signal on the solid substrate with no amplification of target nucleic acid sequence; and (e) detecting the signal decrease on the solid substrate, such that the signal decrease by the cleavage of the immobilized probe is indicative of the presence of the target nucleic acid sequence.