mhurhangee/ep-ind-claims huggingface dataset:
A process for isolating, amplifying and detecting at least one target nucleic acid that may be present in at least one fluid sample, wherein said fluid sample is a body liquid, said process comprising the automated steps of: a. adding an internal control nucleic acid to said fluid sample b. combining together a solid support material and said fluid sample in a vessel for a period of time and under conditions sufficient to permit nucleic acids comprising the target nucleic acid and the internal control nucleic acid to be immobilized on the solid support material c. isolating the solid support material from the other material present in the fluid sample in a separation station d. purifying the nucleic acids in said separation station and washing the solid support material one or more times with a wash buffer e. contacting the purified target nucleic acid and the purified internal control nucleic acid in at least a first vessel and at least one external control nucleic acid in an aqueous buffer in at least a second vessel with one or more amplification reagents f. incubating in said reaction vessels said purified target nucleic acid, said purified internal control nucleic acid and said at least one external control nucleic acid in an aqueous buffer with said one or more amplification reagents for a period of time and under conditions sufficient for an amplification reaction indicative of the presence or absence of said target nucleic acid and said control nucleic acids to occur wherein the conditions for amplification in steps e. to f. are identical for said purified target nucleic acid, said internal control nucleic acid and said at least one external control nucleic acid in an aqueous buffer, and wherein said at least one external control nucleic acid in an aqueous buffer is subjected to the steps following step a. a. adding an internal control nucleic acid to said fluid sample b. combining together a solid support material and said fluid sample in a vessel for a period of time and under conditions sufficient to permit nucleic acids comprising the target nucleic acid and the internal control nucleic acid to be immobilized on the solid support material c. isolating the solid support material from the other material present in the fluid sample in a separation station d. purifying the nucleic acids in said separation station and washing the solid support material one or more times with a wash buffer e. contacting the purified target nucleic acid and the purified internal control nucleic acid in at least a first vessel and at least one external control nucleic acid in an aqueous buffer in at least a second vessel with one or more amplification reagents f. incubating in said reaction vessels said purified target nucleic acid, said purified internal control nucleic acid and said at least one external control nucleic acid in an aqueous buffer with said one or more amplification reagents for a period of time and under conditions sufficient for an amplification reaction indicative of the presence or absence of said target nucleic acid and said control nucleic acids to occur
Stator of a rotary electric generator, the stator comprising: - a magnetic core endowed with a magnetic crown which in turn is endowed with at least one tooth; - a winding wound around the at least one tooth; - an external cooling circuit configured so as to carry a coolant fluid to refrigerate the stator; - at least one through-hole located in the at least one tooth, which longitudinally runs through the tooth between the ends (7, 8) of the tooth; and - first ducts (13, 16) situated in connection with the external cooling circuit and with the at least one through-hole so as to allow the passage of the coolant fluid, the first ducts (13, 16) being interposed between one end (7, 8) of the tooth and the winding; wherein the first ducts (13, 16) comprise: - first inlet ducts configured so as to allow the inlet of coolant fluid from the external cooling circuit towards the at least one through-hole, and - first outlet ducts configured so as to allow the outlet of the coolant fluid from the at least one through-hole towards the cooling circuit; the stator being characterized in that at least one of the first inlet ducts comprises therein a plurality of channels to divert the flow of coolant fluid from first inlets, located in said first inlet ducts, wherefrom the coolant fluid accesses to the first inlet ducts from the external cooling circuit, where the channels flow into the at least one through-hole through first outlets distributed throughout the at least one through-hole, and wherein at least one of the first outlet ducts comprises therein a plurality of channels to divert the flow of coolant fluid from a second inlet, located in said first outlet ducts, wherefrom the coolant fluid accesses to the first outlet ducts from the at least one through-hole, where the channels flow into second outlets connected to the external cooling circuit. - a magnetic core endowed with a magnetic crown which in turn is endowed with at least one tooth; - a winding wound around the at least one tooth; - an external cooling circuit configured so as to carry a coolant fluid to refrigerate the stator; - at least one through-hole located in the at least one tooth, which longitudinally runs through the tooth between the ends (7, 8) of the tooth; and - first ducts (13, 16) situated in connection with the external cooling circuit and with the at least one through-hole so as to allow the passage of the coolant fluid, the first ducts (13, 16) being interposed between one end (7, 8) of the tooth and the winding; - first inlet ducts configured so as to allow the inlet of coolant fluid from the external cooling circuit towards the at least one through-hole, and - first outlet ducts configured so as to allow the outlet of the coolant fluid from the at least one through-hole towards the cooling circuit;
An oncolytic adenoviral vector coding for at least one cytokine together with separate adoptive cell therapeutic composition for use in treatment of cancer, wherein said oncolytic adenoviral vector is i) an adenovirus serotype 5 vector comprising - a 5/3 chimeric fiber knob, - E2F1 promoter for tumor specific expression of E1A, - a 24 bp deletion in the Rb binding constant region 2 of adenoviral E1, - a nucleic acid sequence deletion of viral gp19k and 6.7k reading frames, and - a nucleic acid sequence encoding at least one cytokine transgene in the place of the deleted gp19k / 6.7K in the E3 region resulting in replication-associated control of transgene expression under the viral E3 promoter; or ii) an adenovirus serotype 3 vector comprising - a promoter for tumor specific expression of E1A, - a deletion in the E3 area affecting E3 9 kDa, E3 10.2 kDa, E3 15.2 kDa and E3 15.3 kDa, and - an exogenous or tumor specific promoter for expression of at least one cytokine in the place of the deleted area of E3. i) an adenovirus serotype 5 vector comprising - a 5/3 chimeric fiber knob, - E2F1 promoter for tumor specific expression of E1A, - a 24 bp deletion in the Rb binding constant region 2 of adenoviral E1, - a nucleic acid sequence deletion of viral gp19k and 6.7k reading frames, and - a nucleic acid sequence encoding at least one cytokine transgene in the place of the deleted gp19k / 6.7K in the E3 region resulting in replication-associated control of transgene expression under the viral E3 promoter; or - a 5/3 chimeric fiber knob, - E2F1 promoter for tumor specific expression of E1A, - a 24 bp deletion in the Rb binding constant region 2 of adenoviral E1, - a nucleic acid sequence deletion of viral gp19k and 6.7k reading frames, and - a nucleic acid sequence encoding at least one cytokine transgene in the place of the deleted gp19k / 6.7K in the E3 region resulting in replication-associated control of transgene expression under the viral E3 promoter; or ii) an adenovirus serotype 3 vector comprising - a promoter for tumor specific expression of E1A, - a deletion in the E3 area affecting E3 9 kDa, E3 10.2 kDa, E3 15.2 kDa and E3 15.3 kDa, and - an exogenous or tumor specific promoter for expression of at least one cytokine in the place of the deleted area of E3. - a promoter for tumor specific expression of E1A, - a deletion in the E3 area affecting E3 9 kDa, E3 10.2 kDa, E3 15.2 kDa and E3 15.3 kDa, and - an exogenous or tumor specific promoter for expression of at least one cytokine in the place of the deleted area of E3.
An article of footwear having an upper and a sole structure (30, 30a, 30b) secured to the upper, the sole structure comprising: a fluid-filled chamber (50, 50a, 50b) having an upper surface, an opposite lower surface, and a sidewall extending between the upper surface and the lower surface, the chamber defining a plurality of indented areas in each of the upper surface and the lower surface; an upper sole element (40, 40a, 40b) positioned adjacent to the upper surface of the chamber, the upper sole element having a plurality of projecting areas (44, 44a, 44b) that extend into the indented areas in the upper surface; and a lower sole element (60, 60a, 60b) positioned adjacent to the lower surface, the lower sole element having a plurality of projecting areas (64, 64a, 64b) that extend into the indented areas in the lower surface of the chamber, wherein the sidewall is exposed to form a portion of an exterior surface of the sole structure, and the chamber extends through substantially all of a length of the sole structure, characterised in that the fluid-filled chamber is disposed between the upper sole element and the lower sole element and includes a central subchamber (56, 56a, 56b), a peripheral subchamber (55, 55a, 55b), and a bonded area separating the central subchamber from the peripheral subchamber, the peripheral subchamber extending at least partially around a periphery of the fluid-filled chamber and having (a) a plurality of upper indentations that receive the projections of the upper sole element and (b) a plurality of lower indentations that receive the projections of the lower sole element. a fluid-filled chamber (50, 50a, 50b) having an upper surface, an opposite lower surface, and a sidewall extending between the upper surface and the lower surface, the chamber defining a plurality of indented areas in each of the upper surface and the lower surface; sidewall extending between the upper surface and the lower surface, the chamber defining a plurality of indented areas in each of the upper surface and the lower surface; an upper sole element (40, 40a, 40b) positioned adjacent to the upper surface of the chamber, the upper sole element having a plurality of projecting areas (44, 44a, 44b) that extend into the indented areas in the upper surface; and the upper sole element having a plurality of projecting areas (44, 44a, 44b) that extend into the indented areas in the upper surface; and a lower sole element (60, 60a, 60b) positioned adjacent to the lower surface, the lower sole element having a plurality of projecting areas (64, 64a, 64b) that extend into the indented areas in the lower surface of the chamber, element having a plurality of projecting areas (64, 64a, 64b) that extend into the indented areas in the lower surface of the chamber,
A method for the production of a structured tissue paper comprising providing press-dried single-ply or multi-ply tissue paper and embossing the single-ply or multi-ply tissue paper with an embossing element having a structured surface, whereby a fine structure is produced in the tissue paper, which has an embossing depth which varies along the surface of the tissue paper over a multiplicity of embossing depths perpendicular to the surface of the single-ply or multi-ply tissue paper, characterized in that, during the embossing, a fine structure is produced at least in sections by a multiplicity of first structural elements of the embossing element which are arranged free of interstices on the structured surface of the embossing element, so that the fine structure is free of unembossed interstices.
Method for mounting an oil pan on an assembly, formed by a crankcase and a flywheel housing, of an internal combustion engine, in particular of an internal combustion engine for utility vehicles, wherein the flywheel housing has a first, preferably unitary, parting plane with respect to the crankcase and with respect to the oil pan, in which parting plane, in the mounted state, the respective connecting surfaces of flywheel housing, crankcase and oil pan bear indirectly or directly against one another in an abutting connection, wherein the crankcase furthermore has a second parting plane with respect to the oil pan, in which parting plane, in the mounted state, the connecting surfaces of crankcase and oil pan bear indirectly or directly against one another in an abutting connection, and wherein, firstly, the crankcase and the flywheel housing are connected to form an assembly, to which the oil pan is subsequently fastened, wherein at least one guide means (8, 9; 10, 11) is provided, by means of which at least the connecting surfaces of oil pan and crankcase are, during the connection of the oil pan to the assembly formed by the crankcase and the flywheel housing, oriented relative to one another such that said connecting surfaces are oriented in substantially plane-parallel fashion during the production of the abutting connection between the oil pan and crankcase during the course of the mounting, characterized in that the at least one guide means (8, 9; 10, 11) is arranged between the flywheel housing and the oil pan.
An oral cleaning apparatus, comprising: a body portion having first and second ends (820, 822) and a grip portion situated between the first and second ends (820, 822); an oral cleaning tool coupled to the first end of the body portion and comprising a toothbrush head having first and second groups of bristles (850, 852) of different firmness, the second group of bristles having an adjustable extension; an actuator coupled to the second group of bristle and configured to control an extension of the second group of bristles relative to the first group of bristles; at least one touch-sensitive sensor (104, 106) mounted upon the body portion and which outputs a sensor value indicative of a force applied thereto; and a controller configured to compare the sensor value with a threshold value and actuate the actuator to extend the second group of bristles relative to the first group of bristles based upon the results of the determination. a body portion having first and second ends (820, 822) and a grip portion situated between the first and second ends (820, 822); an oral cleaning tool coupled to the first end of the body portion and comprising a toothbrush head having first and second groups of bristles (850, 852) of different firmness, the second group of bristles having an adjustable extension; an actuator coupled to the second group of bristle and configured to control an extension of the second group of bristles relative to the first group of bristles; at least one touch-sensitive sensor (104, 106) mounted upon the body portion and which outputs a sensor value indicative of a force applied thereto; and a controller configured to compare the sensor value with a threshold value and actuate the actuator to extend the second group of bristles relative to the first group of bristles based upon the results of the determination.
An anchor comprising: a fluke having a head, and a bill for penetrating the ground; and a shank pivotally connected to the fluke so as to be pivotable between an anchor deployment configuration and an anchor retrieval configuration, wherein the shank pivots away from the bill towards the head into the anchor retrieval configuration, characterised in that a fastener is provided at the head end of the fluke to engage with the shank to retain the shank in the anchor retrieval configuration. a fluke having a head, and a bill for penetrating the ground; and a shank pivotally connected to the fluke so as to be pivotable between an anchor deployment configuration and an anchor retrieval configuration, wherein the shank pivots away from the bill towards the head into the anchor retrieval configuration, characterised in that a fastener is provided at the head end of the fluke to engage with the shank to retain the shank in the anchor retrieval configuration.
A device for cataract surgery, comprising a surgical microscope head or stereo microscope head and a laser source, - in which a module, consisting of a laser-coupling/deflecting unit, and/or a laser-scan unit (6,7), and a focusing unit, can be mechanically coupled to the surgical microscope head or stereo microscope head by a fastener, and can be moved by the surgical microscope head or stereo microscope head, - in which at least one of these units can selectively be introduced in an observation beam path between the surgical microscope head or stereo microscope head and an eye, - and in which the focusing unit can scan a depth-of-focus range of greater than 1 mm. - in which a module, consisting of a laser-coupling/deflecting unit, and/or a laser-scan unit (6,7), and a focusing unit, can be mechanically coupled to the surgical microscope head or stereo microscope head by a fastener, and can be moved by the surgical microscope head or stereo microscope head, - in which at least one of these units can selectively be introduced in an observation beam path between the surgical microscope head or stereo microscope head and an eye, - and in which the focusing unit can scan a depth-of-focus range of greater than 1 mm.
A plasticized polymer composition comprising: (a) an N-alkyl-2-pyrrolidone; and (b) a fatty acid ester, wherein said fatty acid ester comprises the methyl ester of octadecenoic acid, the methyl ester of octadecadienoic acid, and the methyl ester of octadecatrienoic acid. (a) an N-alkyl-2-pyrrolidone; and (b) a fatty acid ester,
Exhaust gas recirculation valve having a valve housing and a valve member, which is moveable into closure position or inversely away from it into an opening position via a drive device by means of a lift member guided in a shaft guide against a valve seat area of a valve seat ring, and closes, or opens respectively, the passage to a valve chamber, wherein the valve housing is assembled of several components (120, 140), of which one component at least limits the tightly closed valve chamber and another component comprises a housing part for at least parts of the drive device of the lift member, wherein one component comprises securely and tightly connected body parts which are adjacent to the housing part of the other component, characterized in that a component is formed of an approximately pot-shaped body part having an approximately cylindrical wall part and an end-side valve seat ring being in one piece therewith, and is formed of a housing section, which is united with the housing part of the other component to a one-piece housing unit and is situated opposite of the drive device, wherein the approximately pot-shaped body part is connected to the housing section and the housing section limits the valve chamber together with the approximately pot-shaped body part, wherein the housing unit is designed as die casting part, particularly from aluminium, which housing unit contains in the inside at least one channel for transmitting cooling means, which channel is closely adjacent to the shaft guide.
A parking brake system for an aircraft, comprising: a first switch interface at a first location, comprising a first enable switch and a first activate switch, wherein the parking brake system is configured to switch a state of a brake in response to the first enable switch and the first activate switch being engaged at the same time; and a second switch interface at a second location, comprising a second enable switch and a second activate switch, wherein the parking brake system is configured to switch the state of the brake in response to the second enable switch and the second activate switch being engaged simultaneously; said system further comprising a shutoff valve configured to permit hydraulic fluid to pass in response to at least one of the first enable switch or the second enable switch being engaged, and a servo in fluid communication with the shutoff valve and configured to change the state of the brake between a park state and a not-park state in response to the first activate switch being engaged while the shutoff valve is configured to permit the hydraulic fluid to pass; wherein the shutoff valve 114 is downstream of the servo, wherein the servo is configured to alter the pressure in the brake when the shutoff valve is configured to allow flow to pass and the shutoff valve is configured to lock the hydraulic pressure in the brake in response to the shutoff valve shutting off flow, wherein the parking brake system is configured to retain, in a non-volatile memory, the state of the brake when the parking brake system remains inactive to conserve power. a first switch interface at a first location, comprising a first enable switch and a first activate switch, wherein the parking brake system is configured to switch a state of a brake in response to the first enable switch and the first activate switch being engaged at the same time; and a second switch interface at a second location, comprising a second enable switch and a second activate switch, wherein the parking brake system is configured to switch the state of the brake in response to the second enable switch and the second activate switch being engaged simultaneously; said system further comprising a shutoff valve configured to permit hydraulic fluid to pass in response to at least one of the first enable switch or the second enable switch being engaged, and a servo in fluid communication with the shutoff valve and configured to change the state of the brake between a park state and a not-park state in response to the first activate switch being engaged while the shutoff valve is configured to permit the hydraulic fluid to pass; wherein the shutoff valve 114 is downstream of the servo, wherein the servo is configured to alter the pressure in the brake when the shutoff valve is configured to allow flow to pass and the shutoff valve is configured to lock the hydraulic pressure in the brake in response to the shutoff valve shutting off flow, wherein the parking brake system is configured to retain, in a non-volatile memory, the state of the brake when the parking brake system remains inactive to conserve power.
A method for stabilising biogenic polyamines spermidine, putrescine and cadaverine in soil, organic soil conditioners or fertiliser compositions, characterised by incorporating in the soil, organic soil conditioners or fertiliser compositions an aqueous solution of at least one of said polyamines together with galactomannans.