A light sheet fluorescence microscope (100, 100a, 100b), comprising: a light source device (102, 202, 302) configured to emit excitation light suitable for inducing fluorescent light emitted from a specimen, a detector device (104, 304) configured to detect the fluorescent light from the specimen, and an optical system (106, 306) configured to illuminate the specimen with a light sheet formed from the excitation light and to guide the fluorescent light from the illuminated specimen to the detector device (104, 304), wherein the optical system (106, 306) comprises an objective facing the specimen, the objective being configured to collect the fluorescent light emitted from the specimen, wherein the optical system (106, 306) is configured to illuminate the specimen with the light sheet through the objective, and wherein the light source device (102, 202, 302) is further configured to emit manipulation light suitable for photomanipulating the specimen, wherein the optical system (106, 306) is further configured to direct the manipulation light through the objective through an entrance pupil thereof onto the specimen along a light propagation direction which is different from a light propagation direction of the light sheet, and wherein the optical system (106, 306) comprises a scanning mirror device (116, 316) including at least one tilting mirror (122, 332, 334) which is tiltable for reflecting the excitation light to move the light sheet as a whole through the specimen in a light sheet scanning direction transverse to the light propagation direction of the light sheet, characterized in that the optical system (106, 306) is configured to direct the manipulation light through a spatially limited sub-area of the entrance pupil of the objective on the specimen; wherein the at least one scanning mirror (122, 332, 334) is tiltable for reflecting both the excitation light and the manipulation light. a light source device (102, 202, 302) configured to emit excitation light suitable for inducing fluorescent light emitted from a specimen, a detector device (104, 304) configured to detect the fluorescent light from the specimen, and an optical system (106, 306) configured to illuminate the specimen with a light sheet formed from the excitation light and to guide the fluorescent light from the illuminated specimen to the detector device (104, 304), wherein the optical system (106, 306) comprises an objective facing the specimen, the objective being configured to collect the fluorescent light emitted from the specimen, wherein the optical system (106, 306) is configured to illuminate the specimen with the light sheet through the objective, and wherein the light source device (102, 202, 302) is further configured to emit manipulation light suitable for photomanipulating the specimen, wherein the optical system (106, 306) is further configured to direct the manipulation light through the objective through an entrance pupil thereof onto the specimen along a light propagation direction which is different from a light propagation direction of the light sheet, and wherein the optical system (106, 306) comprises a scanning mirror device (116, 316) including at least one tilting mirror (122, 332, 334) which is tiltable for reflecting the excitation light to move the light sheet as a whole through the specimen in a light sheet scanning direction transverse to the light propagation direction of the light sheet, characterized in that the optical system (106, 306) is configured to direct the manipulation light through a spatially limited sub-area of the entrance pupil of the objective on the specimen; wherein the at least one scanning mirror (122, 332, 334) is tiltable for reflecting both the excitation light and the manipulation light.