A sample analysis substrate rotatable to transfer a liquid, the sample analysis substrate comprising: a substrate including a rotation shaft; a first holding chamber located in the substrate, the first holding chamber having a first space usable to hold a first liquid; a second holding chamber located in the substrate, the second holding chamber having a second space usable to hold the first liquid discharged from the first holding chamber; a main chamber located in the substrate, the main chamber having a third space usable to hold the first liquid discharged from the second holding chamber; a first flow path located in the substrate, the first flow path having a first opening (lllg) and a second opening respectively connected with the first holding chamber and the second holding chamber; and a second flow path located in the substrate, the second flow path having a third opening and a fourth opening respectively connected with the second holding chamber and the main chamber; wherein: the first holding chamber includes a portion that is farther from the rotation shaft than a position thereof connected with the first opening (lllg); the second holding chamber includes a portion that is farther from the rotation shaft than a position thereof connected with the second opening; and in the second flow path, the third opening is closer to the rotation shaft than the fourth opening, characterized in that the first holding chamber and the second flow path are aligned with each other in a radial direction of the substrate, and the second flow path is located farther from the rotation shaft than the first holding chamber, and in that the second holding chamber includes a first portion adjacent to the first holding chamber in a circumferential direction of the substrate and a second portion adjacent to the second flow path in the circumferential direction of the substrate. a substrate including a rotation shaft; a first holding chamber located in the substrate, the first holding chamber having a first space usable to hold a first liquid; a second holding chamber located in the substrate, the second holding chamber having a second space usable to hold the first liquid discharged from the first holding chamber; a main chamber located in the substrate, the main chamber having a third space usable to hold the first liquid discharged from the second holding chamber; a first flow path located in the substrate, the first flow path having a first opening (lllg) and a second opening respectively connected with the first holding chamber and the second holding chamber; and a second flow path located in the substrate, the second flow path having a third opening and a fourth opening respectively connected with the second holding chamber and the main chamber; wherein: the first holding chamber includes a portion that is farther from the rotation shaft than a position thereof connected with the first opening (lllg); the second holding chamber includes a portion that is farther from the rotation shaft than a position thereof connected with the second opening; and in the second flow path, the third opening is closer to the rotation shaft than the fourth opening, characterized in that the first holding chamber and the second flow path are aligned with each other in a radial direction of the substrate, and the second flow path is located farther from the rotation shaft than the first holding chamber, and in that the second holding chamber includes a first portion adjacent to the first holding chamber in a circumferential direction of the substrate and a second portion adjacent to the second flow path in the circumferential direction of the substrate. the first holding chamber includes a portion that is farther from the rotation shaft than a position thereof connected with the first opening (lllg); the second holding chamber includes a portion that is farther from the rotation shaft than a position thereof connected with the second opening; and in the second flow path, the third opening is closer to the rotation shaft than the fourth opening, characterized in that the first holding chamber and the second flow path are aligned with each other in a radial direction of the substrate, and the second flow path is located farther from the rotation shaft than the first holding chamber, and in that the second holding chamber includes a first portion adjacent to the first holding chamber in a circumferential direction of the substrate and a second portion adjacent to the second flow path in the circumferential direction of the substrate.