An acoustic loading system for transporting an analyte in a fluid sample to an analytical instrument, comprising: (a) a reservoir housing a fluid sample containing an analyte, the fluid sample having a fluid surface; (b) a continuous flow sampling probe spaced apart from the fluid surface, comprising (i) a sampling tip for receiving an ejected droplet of the fluid sample, (ii) a solvent inlet for receiving a solvent from a solvent source, (iii) a solvent transport capillary for transporting the solvent from the solvent inlet to the sampling tip, where the ejected droplet combines with the solvent to form an analyte- solvent dilution, (iv) a sample outlet through which the analyte- solvent dilution is directed away from the sampling probe to an analytical instrument, and (v) a sample transport capillary for transporting the analyte- solvent dilution from the sampling tip to the sample outlet, wherein the sample transport capillary and the solvent transport capillary are in fluid communication at the sampling tip; and (c) an acoustic droplet ejector for generating acoustic radiation in a manner effective to successively eject discrete fluid sample droplets from the fluid surface into the sampling tip of the sampling probe; wherein the acoustic droplet ejector comprises an acoustic radiation generator and a focusing means for focusing the acoustic radiation generated at a focal point near the surface of a fluid sample in the reservoir, and further wherein the acoustic droplet ejector is in acoustic coupling relationship with the reservoir. (a) a reservoir housing a fluid sample containing an analyte, the fluid sample having a fluid surface; (b) a continuous flow sampling probe spaced apart from the fluid surface, comprising (i) a sampling tip for receiving an ejected droplet of the fluid sample, (ii) a solvent inlet for receiving a solvent from a solvent source, (iii) a solvent transport capillary for transporting the solvent from the solvent inlet to the sampling tip, where the ejected droplet combines with the solvent to form an analyte- solvent dilution, (iv) a sample outlet through which the analyte- solvent dilution is directed away from the sampling probe to an analytical instrument, and (v) a sample transport capillary for transporting the analyte- solvent dilution from the sampling tip to the sample outlet, wherein the sample transport capillary and the solvent transport capillary are in fluid communication at the sampling tip; and (i) a sampling tip for receiving an ejected droplet of the fluid sample, (ii) a solvent inlet for receiving a solvent from a solvent source, (iii) a solvent transport capillary for transporting the solvent from the solvent inlet to the sampling tip, where the ejected droplet combines with the solvent to form an analyte- solvent dilution, (iv) a sample outlet through which the analyte- solvent dilution is directed away from the sampling probe to an analytical instrument, and (v) a sample transport capillary for transporting the analyte- solvent dilution from the sampling tip to the sample outlet, wherein the sample transport capillary and the solvent transport capillary are in fluid communication at the sampling tip; and (c) an acoustic droplet ejector for generating acoustic radiation in a manner effective to successively eject discrete fluid sample droplets from the fluid surface into the sampling tip of the sampling probe;