A biological fluid collection device, comprising: a housing having a receiving port and an inlet port, the inlet port defining a housing flow channel therein, the housing flow channel in fluid communication with the inlet port; a puncturing element at least partially disposed within the housing and transitionable between a pre-actuated position wherein the puncturing element is retained within the housing and a puncturing position wherein at least a portion of the puncturing element extends through the inlet port of the housing for establishing fluid communication with the housing flow channel; and a cartridge removably connectable to the receiving port of the housing, the cartridge defining a cartridge flow channel therein, and wherein, with the cartridge connected to the housing, the cartridge flow channel is in fluid communication with the housing flow channel, wherein, with the cartridge disconnected from the housing, the cartridge flow channel is sealed, characterized in that the housing flow channel is adapted to receive a multicomponent blood sample having a cellular portion and a plasma portion, the cartridge includes a cartridge inlet port in fluid communication with the housing flow channel when the cartridge is received within the receiving port of the housing and a transfer port in fluid communication with the cartridge flow channel, and the cartridge includes an actuation member in fluid transfer communication with the transfer port, the actuation member transitionable between an initial position in which a multi-component blood sample is stored within the cartridge and an activated position in which at least a portion of the multi-component blood sample is expelled from the transfer port. a housing having a receiving port and an inlet port, the inlet port defining a housing flow channel therein, the housing flow channel in fluid communication with the inlet port; a puncturing element at least partially disposed within the housing and transitionable between a pre-actuated position wherein the puncturing element is retained within the housing and a puncturing position wherein at least a portion of the puncturing element extends through the inlet port of the housing for establishing fluid communication with the housing flow channel; and a cartridge removably connectable to the receiving port of the housing, the cartridge defining a cartridge flow channel therein, and wherein, with the cartridge connected to the housing, the cartridge flow channel is in fluid communication with the housing flow channel, wherein, with the cartridge disconnected from the housing, the cartridge flow channel is sealed, characterized in that the housing flow channel is adapted to receive a multicomponent blood sample having a cellular portion and a plasma portion, the cartridge includes a cartridge inlet port in fluid communication with the housing flow channel when the cartridge is received within the receiving port of the housing and a transfer port in fluid communication with the cartridge flow channel, and the cartridge includes an actuation member in fluid transfer communication with the transfer port, the actuation member transitionable between an initial position in which a multi-component blood sample is stored within the cartridge and an activated position in which at least a portion of the multi-component blood sample is expelled from the transfer port.