A method for identifying a microorganism at species or strain level in a biological sample by polymerase chain reaction, said method comprising the steps of: a) providing a biological sample that has been obtained and which is suspected of comprising microbes, and optionally isolating nucleic acid sequences comprised in said biological sample; b) PCR amplifying at least one microbial rRNA internal transcribed spacer region comprised in said, optionally isolated, nucleic acid sequences using a set of broad-taxonomic range amplification primers for amplifying said at least one microbial rRNA ITS, to thereby generate PCR amplicons; c) recording a high resolution melting curve for the PCR amplicons generated in step b), and recording the length of the PCR amplicons generated in step b); d) comparing the high resolution melting curve recorded in step c) with a database comprising high resolution melting curves of reference amplicons generated from reference microbial species or strains of known taxonomic identity using the same set of amplification primers, to thereby obtain a first taxonomic identity indicator of a microorganism present in said sample; e) comparing the length of each PCR amplicon having a distinct length recorded in step c) with a database comprising PCR amplicon lengths of reference amplicons generated from reference microbial species or strains of known taxonomic identity using the same set of amplification primers, to thereby obtain a second taxonomic identity indicator of a microorganism present in said sample; f) identifying a microorganism present in said sample to the species or strain level if the first and second taxonomic identity indicator match. a) providing a biological sample that has been obtained and which is suspected of comprising microbes, and optionally isolating nucleic acid sequences comprised in said biological sample; b) PCR amplifying at least one microbial rRNA internal transcribed spacer region comprised in said, optionally isolated, nucleic acid sequences using a set of broad-taxonomic range amplification primers for amplifying said at least one microbial rRNA ITS, to thereby generate PCR amplicons; c) recording a high resolution melting curve for the PCR amplicons generated in step b), and recording the length of the PCR amplicons generated in step b); d) comparing the high resolution melting curve recorded in step c) with a database comprising high resolution melting curves of reference amplicons generated from reference microbial species or strains of known taxonomic identity using the same set of amplification primers, to thereby obtain a first taxonomic identity indicator of a microorganism present in said sample; e) comparing the length of each PCR amplicon having a distinct length recorded in step c) with a database comprising PCR amplicon lengths of reference amplicons generated from reference microbial species or strains of known taxonomic identity using the same set of amplification primers, to thereby obtain a second taxonomic identity indicator of a microorganism present in said sample; f) identifying a microorganism present in said sample to the species or strain level if the first and second taxonomic identity indicator match.