An apparatus for depleting at least one of red blood cells, granulocytes, or platelets from a sample comprising blood, bone marrow, or stromal vascular fraction cells separated from adipose tissue, the apparatus comprising: a. a centrifuge having an axis of rotation: b. a rigid cartridge wherein the rigid cartridge comprises: i. a funnel shaped rigid internal chamber having a radius generally inversely proportional to the distance from said axis of rotation when said cartridge is being centrifuged in said centrifuge, and a substantially narrow portion furthest from said axis of rotation when said cartridge is being centrifuged in said centrifuge, an inlet located at the large end of said funnel shaped rigid internal chamber, and an exit port located at the substantially narrow portion of the funnel shaped rigid internal chamber; ii. a first rigid storage compartment in fluid connection with the funnel shaped rigid internal chamber, wherein the first rigid storage compartment is closer to said axis of rotation when said cartridge is being centrifuged in said centrifuge than said exit port; and iii. a second rigid storage compartment in fluid connection with the funnel shaped rigid internal chamber, wherein the second rigid storage compartment is closer to said axis of rotation when said cartridge is being centrifuged in said centrifuge than said exit port; iv. a valve system, configured to urge fluid from the funnel shaped rigid internal chamber into the first or second rigid storage compartment; and c. a control module. a. a centrifuge having an axis of rotation: b. a rigid cartridge wherein the rigid cartridge comprises: i. a funnel shaped rigid internal chamber having a radius generally inversely proportional to the distance from said axis of rotation when said cartridge is being centrifuged in said centrifuge, and a substantially narrow portion furthest from said axis of rotation when said cartridge is being centrifuged in said centrifuge, an inlet located at the large end of said funnel shaped rigid internal chamber, and an exit port located at the substantially narrow portion of the funnel shaped rigid internal chamber; ii. a first rigid storage compartment in fluid connection with the funnel shaped rigid internal chamber, wherein the first rigid storage compartment is closer to said axis of rotation when said cartridge is being centrifuged in said centrifuge than said exit port; and iii. a second rigid storage compartment in fluid connection with the funnel shaped rigid internal chamber, wherein the second rigid storage compartment is closer to said axis of rotation when said cartridge is being centrifuged in said centrifuge than said exit port; iv. a valve system, configured to urge fluid from the funnel shaped rigid internal chamber into the first or second rigid storage compartment; and i. a funnel shaped rigid internal chamber having a radius generally inversely proportional to the distance from said axis of rotation when said cartridge is being centrifuged in said centrifuge, and a substantially narrow portion furthest from said axis of rotation when said cartridge is being centrifuged in said centrifuge, an inlet located at the large end of said funnel shaped rigid internal chamber, and an exit port located at the substantially narrow portion of the funnel shaped rigid internal chamber; a substantially narrow portion furthest from said axis of rotation when said cartridge is being centrifuged in said centrifuge, an inlet located at the large end of said funnel shaped rigid internal chamber, and an exit port located at the substantially narrow portion of the funnel shaped rigid internal chamber; ii. a first rigid storage compartment in fluid connection with the funnel shaped rigid internal chamber, wherein the first rigid storage compartment is closer to said axis of rotation when said cartridge is being centrifuged in said centrifuge than said exit port; and iii. a second rigid storage compartment in fluid connection with the funnel shaped rigid internal chamber, wherein the second rigid storage compartment is closer to said axis of rotation when said cartridge is being centrifuged in said centrifuge than said exit port; iv. a valve system, configured to urge fluid from the funnel shaped rigid internal chamber into the first or second rigid storage compartment; and c. a control module.