mhurhangee/ep-ind-claims huggingface dataset:
A method for producing a recombinant protein, comprising: a high-temperature culture step of culturing at 32°C or higher a Brevibacillus bacterium that contains a gene encoding the recombinant protein; and a low-temperature culture step of culturing the Brevibacillus bacterium at a temperature lower than 32°C after the high-temperature culture step, wherein a shift from the high-temperature culture step to the low-temperature culture step is made between the early and mid-exponential growth phases of the Brevibacillus bacterium, wherein the recombinant protein is an antibody-binding protein. a high-temperature culture step of culturing at 32°C or higher a Brevibacillus bacterium that contains a gene encoding the recombinant protein; and a low-temperature culture step of culturing the Brevibacillus bacterium at a temperature lower than 32°C after the high-temperature culture step, wherein a shift from the high-temperature culture step to the low-temperature culture step is made between the early and mid-exponential growth phases of the Brevibacillus bacterium, wherein the recombinant protein is an antibody-binding protein.
A composition comprising acid oil having free fatty acids and at least one organic acid, wherein the pKa of the acid oil ranges from 2.5 to 4.0 and the organic acid is selected from the group consisting of formic acid, acetic acid, propionic acid, butyric acid, and combinations thereof.
A multi-track cycle comprising: - a pedal generator for generating electric energy to propel the multi-track cycle; and - a base frame, wherein the pedal generator comprises: - a set of pedals (1a, 1b); - a generator mechanically connected to the pedals (1a, 1b); - a further frame for fixing a position of the pedals (1a, 1b) and the generator relative to each other, wherein the further frame is connectable to the base frame by a first connection point and a second connection point, characterized in that the first connection point is connectable to the base frame in alternative positions being discrete or continuously variable, and that a pivotal arm connects the second connection point to the base frame. - a pedal generator for generating electric energy to propel the multi-track cycle; and - a base frame, wherein the pedal generator comprises: - a set of pedals (1a, 1b); - a generator mechanically connected to the pedals (1a, 1b); - a further frame for fixing a position of the pedals (1a, 1b) and the generator relative to each other, wherein the further frame is connectable to the base frame by a first connection point and a second connection point, characterized in that the first connection point is connectable to the base frame in alternative positions being discrete or continuously variable, and that a pivotal arm connects the second connection point to the base frame.
A method for providing cells harvested from a feeder layer comprising: a) providing: i) product cells, wherein said product cells are selected from a group consisting of primary and/or immortalized keratinocytes, ii) feeder layer cells, and iii) an agent that prevents cellular replication; b) treating said feeder layer cells with said agent that prevents cellular replication; c) assaying said feeder layer cells for replication with a proliferation assay; d) discarding said feeder layer cells demonstrating cell replication; e) culturing said product cells on said feeder layer cells; f) separating said product cells and said feeder layer cells, wherein separated product cells are substantially free of residual feeder layer cells, wherein said substantially free of residual feeder layer cells means that the product cells comprise less than 0.001% feeder layer cells on a product cell / feeder layer cell basis; g) assaying said product cells for the presence of feeder layer cell DNA; h) discarding the product cells that are not substantially free of residual feeder layer cells. a) providing: i) product cells, wherein said product cells are selected from a group consisting of primary and/or immortalized keratinocytes, ii) feeder layer cells, and iii) an agent that prevents cellular replication; b) treating said feeder layer cells with said agent that prevents cellular replication; c) assaying said feeder layer cells for replication with a proliferation assay; d) discarding said feeder layer cells demonstrating cell replication; e) culturing said product cells on said feeder layer cells; f) separating said product cells and said feeder layer cells, wherein separated product cells are substantially free of residual feeder layer cells, wherein said substantially free of residual feeder layer cells means that the product cells comprise less than 0.001% feeder layer cells on a product cell / feeder layer cell basis; g) assaying said product cells for the presence of feeder layer cell DNA; h) discarding the product cells that are not substantially free of residual feeder layer cells.
A system for playing a coloring ring back tone, comprising network element devices of: a home location register, a calling user's mobile switching center/visiting location register, a called user's mobile switching center/visiting location register, a state presence server, and a coloring ring back tone control server, wherein the home location register and the calling/called user's mobile switching center/visiting location register are common network element devices in a communication center, wherein there are a physical connection channel and an information interactive interface between the coloring ring back tone control server and the state presence server, wherein the coloring ring back tone control server is configured to obtain the called user's current presence information by interacting information through the information interactive interface, characterized in that the coloring ring back tone control server is configured to play the coloring ring back tone based on a playing priority defined by the called user; the playing priority having the following order to play the coloring ring back tones: based on the called user's current presence information, based on the calling number, and based on the default ring back tone.
A polymer according to formula I wherein, R = R 1 = H or carbon atoms in the range from 1 to 2, R 2 = R 3 = H or carbon atoms in the range from 1 to 5, A = L-PR-Li-B wherein, L = -R 5 -NH-COO-R 6 -R 7 or -R 5 -COO-R 6 -R 7 wherein, Rs is carbon atom in the range from 1 to 6, R 6 is carbon atom in the range 1 to 4, R 7 is sulphur; or R7 is a triazine linked to -CH 2 -CH 2 -N(CH 2 -CH 3)-, PR is a photoresponsive substance, L 1 is carbonate linker, B is a benefit agent, R 4 is A; or R4 is a group selected from -[CH 2 -CH 2 -O-] x, -[CH 2 -CH -O-] x and mixtures thereof, where x is in the range from 1 to 10; m= 1 to 10,000; and n= 1 to 10,000; and wherein the photoresponsive substance is selected from the group consisting of a coumarin compound and a hydroquinone compound. R = R 1 = H or carbon atoms in the range from 1 to 2, R 2 = R 3 = H or carbon atoms in the range from 1 to 5, A = L-PR-Li-B wherein, L = -R 5 -NH-COO-R 6 -R 7 or -R 5 -COO-R 6 -R 7 wherein, Rs is carbon atom in the range from 1 to 6, R 6 is carbon atom in the range 1 to 4, R 7 is sulphur; or R7 is a triazine linked to -CH 2 -CH 2 -N(CH 2 -CH 3)-, PR is a photoresponsive substance, L 1 is carbonate linker, B is a benefit agent, R 4 is A; or R4 is a group selected from -[CH 2 -CH 2 -O-] x, -[CH 2 -CH -O-] x and mixtures thereof, where x is in the range from 1 to 10; m= 1 to 10,000; and n= 1 to 10,000; and wherein the photoresponsive substance is selected from the group consisting of a coumarin compound and a hydroquinone compound.
A disposable bag for a vacuum cleaner, the bag comprising: one or more relatively large sides with a bottom edge; a relatively small base that adjoins the bottom edge of the sides; a collar on the base; and an elongated, light-weight, tube that is disposed within the bag and has a lower end that is connected to the collar, the disposable bag being characterized in that the tube is collapsible and in that the bag is reinforced with a support that extends parallel to the base above an open upper end of the tube and that covers no more than about a half of a top of the bag. one or more relatively large sides with a bottom edge; a relatively small base that adjoins the bottom edge of the sides; a collar on the base; and an elongated, light-weight, tube that is disposed within the bag and has a lower end that is connected to the collar, the disposable bag being characterized in that the tube is collapsible and in that the bag is reinforced with a support that extends parallel to the base above an open upper end of the tube and that covers no more than about a half of a top of the bag.
A two part retraction system comprising: a base paste and a catalyst paste, wherein the base paste comprises a crosslinker capable of releasing hydrogen gas upon combination with the catalyst paste having a hydrosilation catalyst at room temperature, a vinyl fluid, a non-reinforcing filler, a reinforcing filler, optionally a hydroxy terminated polydimethylsiloxane, and optionally a hydrophilic additive, and the catalyst paste comprises a vinyl fluid, a hemostatic agent, a non-reinforcing filler, a reinforcing filler, a platinum complex hydrosilation catalyst, and a retarder, wherein the crosslinker is a polymethylhydrosiloxane, the vinyl fluid is a vinyl terminated organopolysiloxane having at least two vinyl groups per molecule, and the hemostatic agent is selected from the group consisting of ferric sulfate, ferric chloride, a mixture of ferric sulfate and ferric chloride, permanganates, zinc chloride and a water soluble astringent aluminum salt; wherein the hemostatic agent may be encapsulated. a base paste and a catalyst paste, wherein the base paste comprises a crosslinker capable of releasing hydrogen gas upon combination with the catalyst paste having a hydrosilation catalyst at room temperature, a vinyl fluid, a non-reinforcing filler, a reinforcing filler, optionally a hydroxy terminated polydimethylsiloxane, and optionally a hydrophilic additive, and the catalyst paste comprises a vinyl fluid, a hemostatic agent, a non-reinforcing filler, a reinforcing filler, a platinum complex hydrosilation catalyst, and a retarder, wherein the crosslinker is a polymethylhydrosiloxane, the vinyl fluid is a vinyl terminated organopolysiloxane having at least two vinyl groups per molecule, and the hemostatic agent is selected from the group consisting of ferric sulfate, ferric chloride, a mixture of ferric sulfate and ferric chloride, permanganates, zinc chloride and a water soluble astringent aluminum salt; wherein the hemostatic agent may be encapsulated.
An abrasive jet system comprising: an abrasive container; a nozzle assembly (100, 225) having a mixing cavity downstream of a fluid inlet aperture and proximate to an abrasive inlet port (135, 335), wherein the fluid inlet aperture is configured to receive a fluid; and an abrasive supply conduit (120, 220) operably coupleable between the abrasive container and the abrasive inlet port (135, 335), characterized in that the abrasive supply conduit (120, 220) includes a first interior surface portion configured to be positioned proximate to the abrasive inlet port (135, 335) and a second interior surface portion, different from the first interior surface portion, configured to be spaced apart from the abrasive inlet port (135, 335), wherein the first interior surface portion has a different ability to repel the fluid than the second interior surface portion. an abrasive container; a nozzle assembly (100, 225) having a mixing cavity downstream of a fluid inlet aperture and proximate to an abrasive inlet port (135, 335), wherein the fluid inlet aperture is configured to receive a fluid; and an abrasive supply conduit (120, 220) operably coupleable between the abrasive container and the abrasive inlet port (135, 335), characterized in that the abrasive supply conduit (120, 220) includes a first interior surface portion configured to be positioned proximate to the abrasive inlet port (135, 335) and a second interior surface portion, different from the first interior surface portion, configured to be spaced apart from the abrasive inlet port (135, 335), wherein the first interior surface portion has a different ability to repel the fluid than the second interior surface portion.
A yarn-testing device for testing a yarn sample, comprising a yarn path extending along a front of the yarn-testing device, at least one sensor arranged along the yarn path for testing the yarn sample, and a yarn insertion device for inserting the yarn sample into the yarn path, which yarn insertion device comprises a movable gripping device for reversibly gripping the yarn sample, characterized in that the yarn gripping device is on the one hand displaceable in a direction which lies substantially parallel to the yarn path and is on the other hand rotatable about a rotational axis which lies perpendicularly to the yarn path and parallel to a front plane which is defined by the front, wherein the displaceability and the rotatability are independent of each other.
An article transport vehicle comprising: a carrier base configured for movement along a guideway; a pair of gripping arms, each gripping arm having a first end connected to the carrier base and a second end protruding from the carrier base, the pair of gripping arms movable between a closed position configured for holding an article and an open position configured for releasing the article; and a closure mechanism (238, 238') for moving the pair of gripping arms between the closed position and the open position, the closure mechanism (238, 238') biasing the pair of gripping arms to the closed position and moving the pair of gripping arms to the open position when the carrier base is positioned at a predetermined portion of the guideway; characterized in that the second end of each gripping arm has a pair of projections protruding inwardly from an inward lateral surface and upwardly from an upper surface of each gripping arm. a carrier base configured for movement along a guideway; a pair of gripping arms, each gripping arm having a first end connected to the carrier base and a second end protruding from the carrier base, the pair of gripping arms movable between a closed position configured for holding an article and an open position configured for releasing the article; and a closure mechanism (238, 238') for moving the pair of gripping arms between the closed position and the open position, the closure mechanism (238, 238') biasing the pair of gripping arms to the closed position and moving the pair of gripping arms to the open position when the carrier base is positioned at a predetermined portion of the guideway; characterized in that the second end of each gripping arm has a pair of projections protruding inwardly from an inward lateral surface and upwardly from an upper surface of each gripping arm.
A method for wireless communication, performed at a user equipment, UE, the method comprising: selecting, by the UE, configured for cell acquisition for an enhanced machine-type communications, eMTC, protocol type using acquisition signals transmitted by deployments of the eMTC protocol type, to search for acquisition signals (210, 505) associated with a narrow band internet of things, NB-IoT, protocol type; receiving, from a base station, a first acquisition signal (210, 505) of a deployment of the NB-IoT protocol type, the deployment of the NB-IoT protocol type being frequency and time coupled with a deployment of the eMTC protocol type; performing a first portion (305, 365) of cell acquisition for a cell of the deployment of the eMTC protocol type based at least in part on the first acquisition signal (210, 505); receiving, from the base station, a second signal associated with the cell of the deployment of the eMTC protocol type transmitted in resources determined based at least in part on the first portion (305, 365) of the cell acquisition; obtaining system information of the cell of the deployment of the eMTC protocol type based at least in part on the received second signal; and completing the cell acquisition (360, 545) based on the obtained system information. selecting, by the UE, configured for cell acquisition for an enhanced machine-type communications, eMTC, protocol type using acquisition signals transmitted by deployments of the eMTC protocol type, to search for acquisition signals (210, 505) associated with a narrow band internet of things, NB-IoT, protocol type; receiving, from a base station, a first acquisition signal (210, 505) of a deployment of the NB-IoT protocol type, the deployment of the NB-IoT protocol type being frequency and time coupled with a deployment of the eMTC protocol type; performing a first portion (305, 365) of cell acquisition for a cell of the deployment of the eMTC protocol type based at least in part on the first acquisition signal (210, 505); receiving, from the base station, a second signal associated with the cell of the deployment of the eMTC protocol type transmitted in resources determined based at least in part on the first portion (305, 365) of the cell acquisition; obtaining system information of the cell of the deployment of the eMTC protocol type based at least in part on the received second signal; and completing the cell acquisition (360, 545) based on the obtained system information.
Method for providing transport units from a storage facility on at least one storage-removal line, wherein - the transport units are stored in a plurality of storage racks and storage rack levels, wherein the storage racks not arranged on the outside are each arranged in pairs adjoining one another and have a racking storage aisle on one side; - at least one storage-entry line is provided; - the transport units are placed into storage in, and removed from storage from, the storage rack via a storage-entry and storage-removal apparatus for each storage racking aisle; - at least one storage-removal line is provided; - for each storage racking aisle a storage-entry and storage-removal apparatus is provided, - at least one storage-removal lift is used to transfer the transport units to the storage-removal line connected downstream in each case; characterised in that between two adjoining storage racks, a direct exchange of transport units takes place from one storage racking aisle to an adjacent storage racking aisle via transverse conveyance locations in the storage racks, wherein the storage-entry and storage-removal apparatus moves the transport units in the transverse conveyance locations, i.e. there is no further active drive technology in the rack but rather the storage-entry and storage-removal apparatus itself is the single active mechanism for transverse displacement. - the transport units are stored in a plurality of storage racks and storage rack levels, wherein the storage racks not arranged on the outside are each arranged in pairs adjoining one another and have a racking storage aisle on one side; - at least one storage-entry line is provided; - the transport units are placed into storage in, and removed from storage from, the storage rack via a storage-entry and storage-removal apparatus for each storage racking aisle; - at least one storage-removal line is provided; - for each storage racking aisle a storage-entry and storage-removal apparatus is provided, - at least one storage-removal lift is used to transfer the transport units to the storage-removal line connected downstream in each case;
A passive safety device comprising: an inner needle shield having a proximal end, said proximal end being configured to be sealed to a tip of an injection device, and an energy storage portion configured to accumulate energy as the passive safety device passes from the pre-use position to the injection position and to release energy as the passive safety device passes from the injection position to a safety position, a sleeve surrounding at least the proximal end of the inner needle shield, an outer needle shield having a proximal end slidably attached to the sleeve so that the outer needle shield is movable relative to the sleeve, locking means configured to lock the outer needle shield when the passive needle shield reaches the safety position, and wherein the inner needle shield is configured to move the outer needle shield distally as the energy storage portion releases its stored energy; characterized in that the needle shield has a distal portion, said distal portion being configured to be pricked by a needle in a pre-use position of the passive safety device and pierced by the needle in an injection position of the passive safety device. an inner needle shield having a proximal end, said proximal end being configured to be sealed to a tip of an injection device, and an energy storage portion configured to accumulate energy as the passive safety device passes from the pre-use position to the injection position and to release energy as the passive safety device passes from the injection position to a safety position, a sleeve surrounding at least the proximal end of the inner needle shield, an outer needle shield having a proximal end slidably attached to the sleeve so that the outer needle shield is movable relative to the sleeve, locking means configured to lock the outer needle shield when the passive needle shield reaches the safety position, and wherein the inner needle shield is configured to move the outer needle shield distally as the energy storage portion releases its stored energy; characterized in that the needle shield has a distal portion, said distal portion being configured to be pricked by a needle in a pre-use position of the passive safety device and pierced by the needle in an injection position of the passive safety device.
An airflow testing mold (14, 16) suitable for mating with a workpiece, the mold comprising: a platform portion formed from a first material having a first hardness; and a workpiece mating portion surrounded by the platform portion, the mating portion comprising: a support region abutting the platform portion and formed from a second material having a second hardness; and a sealing surface positioned above the support region and formed from a third material having a third hardness, the sealing surface being configured to mate with and extend into a hollow portion of the workpiece; wherein the first hardness is different from at least one of the second and third hardnesses; and characterised in that the first, second and third hardnesses are Shore A durometer hardnesses and the first hardness differs from at least one of the second and third hardnesses by at least 10 Shore A points. a platform portion formed from a first material having a first hardness; and a workpiece mating portion surrounded by the platform portion, the mating portion comprising: a support region abutting the platform portion and formed from a second material having a second hardness; and a sealing surface positioned above the support region and formed from a third material having a third hardness, the sealing surface being configured to mate with and extend into a hollow portion of the workpiece; a support region abutting the platform portion and formed from a second material having a second hardness; and a sealing surface positioned above the support region and formed from a third material having a third hardness, the sealing surface being configured to mate with and extend into a hollow portion of the workpiece; wherein the first hardness is different from at least one of the second and third hardnesses; and characterised in that the first, second and third hardnesses are Shore A durometer hardnesses and the first hardness differs from at least one of the second and third hardnesses by at least 10 Shore A points.