A sterile fluid pathway assembly comprising: a sterile fluid container (50, 150, 350); a sterile fluid conduit (35, 135, 235, 335); and a sterile fluid connector (30, 130, 230, 330) comprising a first portion configured to connect the sterile fluid conduit, and a second portion comprising a housing (52, 352, 452) configured to mount the sterile fluid container; a connector hub (31, 131, 231, 331, 431, 531); a pierceable seal (56, 156, 356, 456, 556) disposed at least partially between the connector hub and a sterile fluid chamber (21, 121A, 121B, 121C, 321) of the sterile fluid container, the pierceable seal in sealing contact with the connector hub to form a sterile cavity between the connector hub and the pierceable seal, wherein the sterile cavity is formed at least partially internal to the sterile fluid container; and a piercing member (33, 133, 233, 333, 433, 533, 633) fixedly disposed within the connector hub capable of providing a sterile fluid communication between the sterile fluid conduit and the sterile fluid chamber of the sterile fluid container, the piercing member being stationary; wherein at least a portion of the pierceable seal is configured to transform from a non-activated state wherein the pierceable seal is intact, to an activated state wherein the pierceable seal is disrupted by the piercing member to create a sterile fluid communication between the sterile fluid conduit and the sterile fluid chamber of the sterile fluid container; and wherein the sterile fluid container comprises a plunger seal (60, 160, 260, 360) and translation of the plunger seal results in relative motion of the pierceable seal towards the piercing member. a sterile fluid container (50, 150, 350); a sterile fluid conduit (35, 135, 235, 335); and a sterile fluid connector (30, 130, 230, 330) comprising a first portion configured to connect the sterile fluid conduit, and a second portion comprising a housing (52, 352, 452) configured to mount the sterile fluid container; a connector hub (31, 131, 231, 331, 431, 531); a pierceable seal (56, 156, 356, 456, 556) disposed at least partially between the connector hub and a sterile fluid chamber (21, 121A, 121B, 121C, 321) of the sterile fluid container, the pierceable seal in sealing contact with the connector hub to form a sterile cavity between the connector hub and the pierceable seal, wherein the sterile cavity is formed at least partially internal to the sterile fluid container; and a piercing member (33, 133, 233, 333, 433, 533, 633) fixedly disposed within the connector hub capable of providing a sterile fluid communication between the sterile fluid conduit and the sterile fluid chamber of the sterile fluid container, the piercing member being stationary; a first portion configured to connect the sterile fluid conduit, and a second portion comprising a housing (52, 352, 452) configured to mount the sterile fluid container; a connector hub (31, 131, 231, 331, 431, 531); a pierceable seal (56, 156, 356, 456, 556) disposed at least partially between the connector hub and a sterile fluid chamber (21, 121A, 121B, 121C, 321) of the sterile fluid container, the pierceable seal in sealing contact with the connector hub to form a sterile cavity between the connector hub and the pierceable seal, wherein the sterile cavity is formed at least partially internal to the sterile fluid container; and a piercing member (33, 133, 233, 333, 433, 533, 633) fixedly disposed within the connector hub capable of providing a sterile fluid communication between the sterile fluid conduit and the sterile fluid chamber of the sterile fluid container, the piercing member being stationary; wherein at least a portion of the pierceable seal is configured to transform from a non-activated state wherein the pierceable seal is intact, to an activated state wherein the pierceable seal is disrupted by the piercing member to create a sterile fluid communication between the sterile fluid conduit and the sterile fluid chamber of the sterile fluid container; and wherein the sterile fluid container comprises a plunger seal (60, 160, 260, 360) and translation of the plunger seal results in relative motion of the pierceable seal towards the piercing member.