A puncture frame comprising one or more needle groove bodies and a puncture frame body, the needle groove body can be installed to a puncture frame body to be fixed to an ultrasonic probe during a puncture operation, characterized in that the needle groove body comprises a first groove plate, a first fixing plate, a limiting plate, and a second fixing plate which are connected in sequence such that the needle groove body is of a hollow parallelepiped structure and the needle groove body when placed on the puncture frame body, forms a u-shaped needle groove suitable to guide the movement of a puncture needle, wherein the u-shaped needle groove is formed by the first groove plate, a second groove plate of the puncture frame body and an inclined mounting plate of the puncture frame body, the puncture frame body for use together with the needle groove body; wherein the puncture frame body has a connecting portion at one end and a sleeve-shaped portion at the other end, and the second groove plate and the inclined mounting plate are provided on the connecting portion and are perpendicular to each other, wherein, the inclined mounting plate is provided with a first rib and a second rib which are parallel to each other and cooperate with the first fixing plate and the second fixing plate of the needle groove body, respectively, when the puncture frame body is used together with the needle groove body, the inclined mounting plate is fitted with the bottom of the needle groove body, the needle groove body can be sleeved outside the first rib and the second rib, and during the puncture operation, the ultrasonic probe can be inserted into and fixed with the sleeve-shaped portion such that the puncture frame body can be fixed to the ultrasonic probe. the needle groove body can be installed to a puncture frame body to be fixed to an ultrasonic probe during a puncture operation, characterized in that the needle groove body comprises a first groove plate, a first fixing plate, a limiting plate, and a second fixing plate which are connected in sequence such that the needle groove body is of a hollow parallelepiped structure and the needle groove body when placed on the puncture frame body, forms a u-shaped needle groove suitable to guide the movement of a puncture needle, wherein the u-shaped needle groove is formed by the first groove plate, a second groove plate of the puncture frame body and an inclined mounting plate of the puncture frame body, a first groove plate, a first fixing plate, a limiting plate, and a second fixing plate which are connected in sequence such that the needle groove body is of a hollow parallelepiped structure and the needle groove body when placed on the puncture frame body, forms a u-shaped needle groove suitable to guide the movement of a puncture needle, wherein the u-shaped needle groove is formed by the first groove plate, a second groove plate of the puncture frame body and an inclined mounting plate of the puncture frame body, the puncture frame body for use together with the needle groove body; wherein the puncture frame body has a connecting portion at one end and a sleeve-shaped portion at the other end, and the second groove plate and the inclined mounting plate are provided on the connecting portion and are perpendicular to each other, wherein, the inclined mounting plate is provided with a first rib and a second rib which are parallel to each other and cooperate with the first fixing plate and the second fixing plate of the needle groove body, respectively, when the puncture frame body is used together with the needle groove body, the inclined mounting plate is fitted with the bottom of the needle groove body, the needle groove body can be sleeved outside the first rib and the second rib, and during the puncture operation, the ultrasonic probe can be inserted into and fixed with the sleeve-shaped portion such that the puncture frame body can be fixed to the ultrasonic probe.