method for generating a myocardial region of interest in nuclear imaging comprising: obtaining nuclear medicine image data, wherein said nuclear medicine image data include volumetric data indicative of a region of interest including a region of the heart; orientating the nuclear medicine image data to provide a first cardiac long axis view, a second cardiac long axis view, and a plurality of cardiac short axis views; displaying the first cardiac long axis view in a human readable form; receiving a first human input identifying a plurality of first locations of myocardial tissue in the first cardiac long axis view; connecting the first locations by a plurality of first line segments; determining first intersections of the plurality of first line segments with each of the plurality of cardiac short axis views; displaying the first locations and the first line segments in the first cardiac long axis view and displaying the first intersections in a selected one of the cardiac short axis views to allow the user to review and adjust the first locations and/or the first line segments; displaying the second cardiac long axis view in a human readable form; receiving a second human input identifying a plurality of second locations of the myocardium in the second cardiac long axis view; connecting the second locations by a plurality of second line segments; determining second intersections of the plurality of second line segments with each of the plurality of the cardiac short axis views; displaying the second locations and the second line segments in the second cardiac long axis view and displaying the second intersections in the selected one of the cardiac short axis views to allow the user to review and adjust the second locations and/or the second line segments; identifying a cardiac basal plane and an apex; automatically constructing a myocardial tissue surface by (i) identifying the first and the second intersections (306, 312) in each of a desired plurality of the cardiac short axis views between the cardiac basal plane and the apex, and by (ii) connecting the adjacent first and the second intersections (306, 312) with each other in each of the desired plurality of cardiac short axis views using a mathematical model to model the myocardial tissue surface in the desired plurality of cardiac short axis views; expanding the myocardial tissue surface to a desired thickness to generate a myocardial region of interest in each of the plurality of cardiac short axis views. obtaining nuclear medicine image data, wherein said nuclear medicine image data include volumetric data indicative of a region of interest including a region of the heart; orientating the nuclear medicine image data to provide a first cardiac long axis view, a second cardiac long axis view, and a plurality of cardiac short axis views; displaying the first cardiac long axis view in a human readable form; receiving a first human input identifying a plurality of first locations of myocardial tissue in the first cardiac long axis view; connecting the first locations by a plurality of first line segments; determining first intersections of the plurality of first line segments with each of the plurality of cardiac short axis views; displaying the first locations and the first line segments in the first cardiac long axis view and displaying the first intersections in a selected one of the cardiac short axis views to allow the user to review and adjust the first locations and/or the first line segments; displaying the second cardiac long axis view in a human readable form; receiving a second human input identifying a plurality of second locations of the myocardium in the second cardiac long axis view; connecting the second locations by a plurality of second line segments; determining second intersections of the plurality of second line segments with each of the plurality of the cardiac short axis views; displaying the second locations and the second line segments in the second cardiac long axis view and displaying the second intersections in the selected one of the cardiac short axis views to allow the user to review and adjust the second locations and/or the second line segments; identifying a cardiac basal plane and an apex; automatically constructing a myocardial tissue surface by (i) identifying the first and the second intersections (306, 312) in each of a desired plurality of the cardiac short axis views between the cardiac basal plane and the apex, and by (ii) connecting the adjacent first and the second intersections (306, 312) with each other in each of the desired plurality of cardiac short axis views using a mathematical model to model the myocardial tissue surface in the desired plurality of cardiac short axis views; expanding the myocardial tissue surface to a desired thickness to generate a myocardial region of interest in each of the plurality of cardiac short axis views.