A method of substantially removing magnetically responsive beads from liquid portions or droplets in digital microfluidics, wherein the method comprises the steps of: a) providing a digital microfluidics system comprising a number or array of individual electrodes attached to a first substrate, a first hydrophobic surface located on said individual electrodes, and a central control unit in operative contact with said individual electrodes for controlling selection and for providing a number of said individual electrodes with voltage for manipulating liquid portions or liquid droplets by electrowetting; b) providing in the first substrate of the microfluidics system and below said individual electrodes at least one magnetic conduit comprising a backside and being configured to be backed by a backing magnet with a magnetic field and being configured for directing said magnetic field through the magnetic conduit to the first hydrophobic surface on said individual electrodes, said at least one magnetic conduit being located in close proximity to individual electrodes; c) providing on the hydrophobic surfaces and above a path of selected electrodes at least one liquid portion or liquid droplet that comprises magnetically responsive beads; d) moving by electrowetting said at least one liquid portion or liquid droplet with the magnetically responsive beads on said path of selected electrodes until at least a part of said at least one liquid portion or liquid droplet is placed atop of at least one specific magnetic conduit; e) actuating the backing magnet so that it is operatively backing the at least one specific magnetic conduit, and thus attracting magnetically responsive beads of said at least one liquid portion or liquid droplet through directing said magnetic field to the first hydrophobic surface on said individual electrodes by the at least one specific magnetic conduit and concentrating attracted magnetically responsive beads; and f) while actuating the backing magnet, moving by electrowetting said at least one liquid portion ' or liquid droplet ' with a substantially decreased number of magnetically responsive beads on said path of selected electrodes away from the specific magnetic conduit. a) providing a digital microfluidics system comprising a number or array of individual electrodes attached to a first substrate, a first hydrophobic surface located on said individual electrodes, and a central control unit in operative contact with said individual electrodes for controlling selection and for providing a number of said individual electrodes with voltage for manipulating liquid portions or liquid droplets by electrowetting; b) providing in the first substrate of the microfluidics system and below said individual electrodes at least one magnetic conduit comprising a backside and being configured to be backed by a backing magnet with a magnetic field and being configured for directing said magnetic field through the magnetic conduit to the first hydrophobic surface on said individual electrodes, said at least one magnetic conduit being located in close proximity to individual electrodes; c) providing on the hydrophobic surfaces and above a path of selected electrodes at least one liquid portion or liquid droplet that comprises magnetically responsive beads; d) moving by electrowetting said at least one liquid portion or liquid droplet with the magnetically responsive beads on said path of selected electrodes until at least a part of said at least one liquid portion or liquid droplet is placed atop of at least one specific magnetic conduit; e) actuating the backing magnet so that it is operatively backing the at least one specific magnetic conduit, and thus attracting magnetically responsive beads of said at least one liquid portion or liquid droplet through directing said magnetic field to the first hydrophobic surface on said individual electrodes by the at least one specific magnetic conduit and concentrating attracted magnetically responsive beads; and f) while actuating the backing magnet, moving by electrowetting said at least one liquid portion ' or liquid droplet ' with a substantially decreased number of magnetically responsive beads on said path of selected electrodes away from the specific magnetic conduit.