A method for in vivo, full-field interference microscopy imaging of a scattering three-dimensional sample, comprising: - disposing the sample in an object arm of an interference device of a full-field OCT imaging system, wherein said interference device further comprises a reference arm with an optical lens and a first reflection surface; - producing, at each point of an imaging field, an interference between a reference wave obtained by reflection of incident light waves on an elementary surface of the first reflection surface corresponding to said point of the imaging field and an object wave obtained by backscattering of incident light waves by a voxel of a slice of the sample at a given depth, said voxel corresponding to said point of the imaging field, - acquiring, using an acquisition device of said full-field OCT imaging system, a temporal succession of two-dimensional interferometric signals resulting from the interferences produced at each point of the imaging field; - storing, for each two-dimensional interferometric signal, a time of acquisition; - providing, at each time of acquisition of the two-dimensional interferometric signals, cross-sectional images (X - Z) of both the sample and said first reflection surface of said full-field OCT imaging system using an OCT imaging system; - determining a plurality of en face images (X - Y) of a plurality of slices of the sample, each en face image being determined from at least two two-dimensional interferometric signals (I 1, I 2) having a given phase shift; - determining from the cross-sectional images provided by the OCT imaging system at the times of acquisition of each of said two two-dimensional interferometric signals (I 1, I 2) a depth (z) for each en face image (X - Y) of said plurality of slices; characterized in that: - determining each en face image comprises selecting, in said temporal succession of two-dimensional interferometric signals acquired by the acquisition device, at least two two-dimensional interferometric signals (I 1, I 2) having said phase shift, wherein said phase shift is induced by in vivo movements of the sample; and characterized in that the method further comprises: - determining a 3D image of the sample from said plurality of en face images of said plurality of slices of the sample and depths. - disposing the sample in an object arm of an interference device of a full-field OCT imaging system, wherein said interference device further comprises a reference arm with an optical lens and a first reflection surface; - producing, at each point of an imaging field, an interference between a reference wave obtained by reflection of incident light waves on an elementary surface of the first reflection surface corresponding to said point of the imaging field and an object wave obtained by backscattering of incident light waves by a voxel of a slice of the sample at a given depth, said voxel corresponding to said point of the imaging field, - acquiring, using an acquisition device of said full-field OCT imaging system, a temporal succession of two-dimensional interferometric signals resulting from the interferences produced at each point of the imaging field; - storing, for each two-dimensional interferometric signal, a time of acquisition; - providing, at each time of acquisition of the two-dimensional interferometric signals, cross-sectional images (X - Z) of both the sample and said first reflection surface of said full-field OCT imaging system using an OCT imaging system; - determining a plurality of en face images (X - Y) of a plurality of slices of the sample, each en face image being determined from at least two two-dimensional interferometric signals (I 1, I 2) having a given phase shift; - determining from the cross-sectional images provided by the OCT imaging system at the times of acquisition of each of said two two-dimensional interferometric signals (I 1, I 2) a depth (z) for each en face image (X - Y) of said plurality of slices; characterized in that: - determining each en face image comprises selecting, in said temporal succession of two-dimensional interferometric signals acquired by the acquisition device, at least two two-dimensional interferometric signals (I 1, I 2) having said phase shift, wherein said phase shift is induced by in vivo movements of the sample; and characterized in that the method further comprises: - determining a 3D image of the sample from said plurality of en face images of said plurality of slices of the sample and depths. - determining each en face image comprises selecting, in said temporal succession of two-dimensional interferometric signals acquired by the acquisition device, at least two two-dimensional interferometric signals (I 1, I 2) having said phase shift, wherein said phase shift is induced by in vivo movements of the sample; and characterized in that the method further comprises: - determining a 3D image of the sample from said plurality of en face images of said plurality of slices of the sample and depths. - determining a 3D image of the sample from said plurality of en face images of said plurality of slices of the sample and depths.