A tunable multiple laser pulse scanning microscope applying at least two pulsed laser beams with distinct wavelengths incident both simultaneously or at a specific time delay on a scanning spot of a sample to be imaged, comprising: a first pulsed laser light source providing a first pulsed laser beam of a first distinct wavelength; a second pulsed laser light source (1; 2) providing a second pulsed laser beam of a second distinct wavelength; an acousto-optic tunable filter, AOTF, for tuning at least one of the first and second pulsed laser beams sent through the acousto-optic tunable filter; a delay stage provided a) upstream of the acousto-optic tunable filter or b) in parallel to the acousto-optic tunable filter for delaying the at least one of the first and second pulsed laser beams; and a delay detector for detecting a time delay between the first and second pulsed laser beams downstream of the acousto-optic tunable filter and a) a motorized actuator (25, 26) that is configured to move the delay stage depending on the time delay between the first and second pulsed laser beam downstream of the acousto-optic tunable filter and a control unit for controlling the motorized actuator (25, 26) depending on the time delay, or b) a manually operated actuator and a look-up table, whereby the manually operated actuator is configured such that the position of the delay stage is adjustable to a position according to a position that can be read from the look-up table depending on the time delay between the first and second pulsed laser beam downstream of the acousto-optic tunable filter. a first pulsed laser light source providing a first pulsed laser beam of a first distinct wavelength; a second pulsed laser light source (1; 2) providing a second pulsed laser beam of a second distinct wavelength; an acousto-optic tunable filter, AOTF, for tuning at least one of the first and second pulsed laser beams sent through the acousto-optic tunable filter; a delay stage provided a) upstream of the acousto-optic tunable filter or b) in parallel to the acousto-optic tunable filter for delaying the at least one of the first and second pulsed laser beams; and a) upstream of the acousto-optic tunable filter or b) in parallel to the acousto-optic tunable filter for delaying the at least one of the first and second pulsed laser beams; and a delay detector for detecting a time delay between the first and second pulsed laser beams downstream of the acousto-optic tunable filter and a) a motorized actuator (25, 26) that is configured to move the delay stage depending on the time delay between the first and second pulsed laser beam downstream of the acousto-optic tunable filter and a control unit for controlling the motorized actuator (25, 26) depending on the time delay, or b) a manually operated actuator and a look-up table, whereby the manually operated actuator is configured such that the position of the delay stage is adjustable to a position according to a position that can be read from the look-up table depending on the time delay between the first and second pulsed laser beam downstream of the acousto-optic tunable filter. a) a motorized actuator (25, 26) that is configured to move the delay stage depending on the time delay between the first and second pulsed laser beam downstream of the acousto-optic tunable filter and a control unit for controlling the motorized actuator (25, 26) depending on the time delay, or b) a manually operated actuator and a look-up table, whereby the manually operated actuator is configured such that the position of the delay stage is adjustable to a position according to a position that can be read from the look-up table depending on the time delay between the first and second pulsed laser beam downstream of the acousto-optic tunable filter.