A real-time and quantitative measurement method for cell traction force, comprising the following steps: placing an AT-cut quartz crystal and a BT-cut quartz crystal in culture dishes or detection cells, wherein the AT-cut quartz crystal has the same frequency and the same surface morphology as those of the BT-cut quartz crystal; and adding live cells to be tested to the culture dishes or the detection cells, and measuring the cell traction force ΔSt of the live cells at an adhesion time t by the following formula: Δ S t = K AT − K BT − 1 t q AT Δ f t AT / fr AT − tq BT Δ f t BT / fr BT wherein K AT = 2.75× 10 -7 cm 2 /N (2.75 × 10 -12 cm 2 dyn -1) and K BT = -2.65 × 10 -7 cm 2 /N (-2.65 × 10 -12 cm 2 dyn -1) are stress coefficients of the AT-cut quartz crystal and the BT-cut quartz crystal respectively; fr AT is the resonant frequency of the AT-cut quartz crystal, fr BT is the resonant frequency of the BT-cut quartz crystal, tq AT is the thickness of the AT-cut quartz crystal, tq BT is the thickness of the BT-cut quartz crystal, and all of which are constants; Δf t AT and Δf t BT are the frequency shifts of the AT-cut and BT-cut quartz crystals at any time t relative to their reference points respectively; wherein when ΔSt is negative, it indicates that the stress on the live cells is a compressive stress, the live cells are contracted, and the corresponding extracellular matrix is subjected to a tensile stress equal and opposite to the compressive stress; and when ΔS t is positive, the stress on the live cells is a tensile stress, the live cells are spread, and the corresponding extracellular matrix is subjected to a compressive stress equal and opposite to the tensile stress. placing an AT-cut quartz crystal and a BT-cut quartz crystal in culture dishes or detection cells, wherein the AT-cut quartz crystal has the same frequency and the same surface morphology as those of the BT-cut quartz crystal; and adding live cells to be tested to the culture dishes or the detection cells, and measuring the cell traction force ΔSt of the live cells at an adhesion time t by the following formula: Δ S t = K AT − K BT − 1 t q AT Δ f t AT / fr AT − tq BT Δ f t BT / fr BT wherein K AT = 2.75× 10 -7 cm 2 /N (2.75 × 10 -12 cm 2 dyn -1) and K BT = -2.65 × 10 -7 cm 2 /N (-2.65 × 10 -12 cm 2 dyn -1) are stress coefficients of the AT-cut quartz crystal and the BT-cut quartz crystal respectively; fr AT is the resonant frequency of the AT-cut quartz crystal, fr BT is the resonant frequency of the BT-cut quartz crystal, tq AT is the thickness of the AT-cut quartz crystal, tq BT is the thickness of the BT-cut quartz crystal, and all of which are constants; Δf t AT and Δf t BT are the frequency shifts of the AT-cut and BT-cut quartz crystals at any time t relative to their reference points respectively; wherein when ΔSt is negative, it indicates that the stress on the live cells is a compressive stress, the live cells are contracted, and the corresponding extracellular matrix is subjected to a tensile stress equal and opposite to the compressive stress; and when ΔS t is positive, the stress on the live cells is a tensile stress, the live cells are spread, and the corresponding extracellular matrix is subjected to a compressive stress equal and opposite to the tensile stress. wherein K AT = 2.75× 10 -7 cm 2 /N (2.75 × 10 -12 cm 2 dyn -1) and K BT = -2.65 × 10 -7 cm 2 /N (-2.65 × 10 -12 cm 2 dyn -1) are stress coefficients of the AT-cut quartz crystal and the BT-cut quartz crystal respectively; fr AT is the resonant frequency of the AT-cut quartz crystal, fr BT is the resonant frequency of the BT-cut quartz crystal, tq AT is the thickness of the AT-cut quartz crystal, tq BT is the thickness of the BT-cut quartz crystal, and all of which are constants; Δf t AT and Δf t BT are the frequency shifts of the AT-cut and BT-cut quartz crystals at any time t relative to their reference points respectively; wherein when ΔSt is negative, it indicates that the stress on the live cells is a compressive stress, the live cells are contracted, and the corresponding extracellular matrix is subjected to a tensile stress equal and opposite to the compressive stress; and when ΔS t is positive, the stress on the live cells is a tensile stress, the live cells are spread, and the corresponding extracellular matrix is subjected to a compressive stress equal and opposite to the tensile stress.