A system for acquiring multiple fluorescent images, the system comprising: a pen-shaped medical fluorescent imaging device comprising a probe configured to extend in a longitudinal direction and include an image capturing section at one end; a plurality of light sources (130, 131, 132, 133) configured to be arranged to surround the image capturing section; and a controller configured to control the light sources (130, 131, 132, 133), wherein the light sources (130, 131, 132, 133) include a first light source configured to emit light of a first wavelength range so that blood vessels can be marked by a first fluorescent material, and a second light source configured to emit light of a second wavelength range so that a glioma can be marked by a second fluorescent material, and the first and second light sources are selectively controllable by the controller; characterized in that the system further comprises a fluorescent multi-image acquirer configured to acquire an actual image of a target part, a fluorescent vascular image of blood vessels marked by the first fluorescent material, and a fluorescent glioma image of a glioma marked by the second fluorescent material; a blood vessel extractor configured to extract a vascular shape from the acquired fluorescent vascular image; a glioma image processor configured to process the acquired fluorescent glioma image so that a boundary of the glioma can become distinct; a fluorescent multi-image aligner configured to align the fluorescent vascular image and the fluorescent glioma image in real time; and a fluorescent image display configured to display a fluorescent multi-image in which the fluorescent vascular image and the fluorescent glioma image are aligned, wherein the fluorescent multi-image aligner is configured to measure pixel value-based similarities of a moving image which is the fluorescent vascular image or the fluorescent glioma image with respect to a fixed image which is the actual image and to select the transformation parameters corresponding to the highest similarity to transform the fluorescent vascular image or the fluorescent glioma image to match the actual image using [Equation 1]; wherein the transformation parameters comprise a scale factor, a radian-based angle, x and y values of a center position (x, y) of the fluorescent vascular image or the fluorescent glioma image after transformation, and translated x and y values (x', y'): x ′ y ′ = λ 0 0 λ cos θ − sin θ sin θ cos θ x − C x y − C y + T x + C x T y + C y wherein λ is a scale factor, θ is a rotation angle, (C x, C y) are values of a rotation center position, and (T x, T y) are values of translated elements. a pen-shaped medical fluorescent imaging device comprising a probe configured to extend in a longitudinal direction and include an image capturing section at one end; a plurality of light sources (130, 131, 132, 133) configured to be arranged to surround the image capturing section; and a controller configured to control the light sources (130, 131, 132, 133), wherein the light sources (130, 131, 132, 133) include a first light source configured to emit light of a first wavelength range so that blood vessels can be marked by a first fluorescent material, and a second light source configured to emit light of a second wavelength range so that a glioma can be marked by a second fluorescent material, and the first and second light sources are selectively controllable by the controller; characterized in that the system further comprises a fluorescent multi-image acquirer configured to acquire an actual image of a target part, a fluorescent vascular image of blood vessels marked by the first fluorescent material, and a fluorescent glioma image of a glioma marked by the second fluorescent material; a blood vessel extractor configured to extract a vascular shape from the acquired fluorescent vascular image; a glioma image processor configured to process the acquired fluorescent glioma image so that a boundary of the glioma can become distinct; a fluorescent multi-image aligner configured to align the fluorescent vascular image and the fluorescent glioma image in real time; and a fluorescent image display configured to display a fluorescent multi-image in which the fluorescent vascular image and the fluorescent glioma image are aligned, wherein the fluorescent multi-image aligner is configured to measure pixel value-based similarities of a moving image which is the fluorescent vascular image or the fluorescent glioma image with respect to a fixed image which is the actual image and to select the transformation parameters corresponding to the highest similarity to transform the fluorescent vascular image or the fluorescent glioma image to match the actual image using [Equation 1]; wherein the transformation parameters comprise a scale factor, a radian-based angle, x and y values of a center position (x, y) of the fluorescent vascular image or the fluorescent glioma image after transformation, and translated x and y values (x', y'): x ′ y ′ = λ 0 0 λ cos θ − sin θ sin θ cos θ x − C x y − C y + T x + C x T y + C y wherein λ is a scale factor, θ is a rotation angle, (C x, C y) are values of a rotation center position, and (T x, T y) are values of translated elements.