A microscope, comprising: an autofocus system for executing a focusing procedure, having a first image sensor, arranged in a first coupled-out beam path, for acquiring a first image and a second image sensor, arranged in a second coupled-out beam path, for acquiring a second image, the autofocus system being configured such that the focusing procedure is executable on the basis of a first operating mode and a second operating mode, the autofocus system being configured such that in the first operating mode the autofocus system ascertains contrast values of the first image acquired by the first image sensor and of the second image acquired by the second image sensor, and in the second operating mode the autofocus system ascertains contrast values of the first image acquired by the first image sensor and of the second image acquired by the second image sensor, and sets a relative location of a focal plane with respect to an object plane based on the ascertained contrast values, the autofocus system being configured to set the relative location of the focal plane with respect to the object plane in the first operating mode such that the focal plane lies within a first tolerance region around the object plane and the relative location of the focal plane with respect to the object plane in the second operating mode such that the focal plane lies within a second tolerance region around the object plane, and the second tolerance region being smaller than the first tolerance region, characterized in that the autofocus system is configured such that the focusing procedure executable based on the first operating mode is carried out only one single time for finding the focus, and the focusing procedure for holding the focus, executable based on the second operating mode, is repeated until a predefined termination condition is satisfied. an autofocus system for executing a focusing procedure, having a first image sensor, arranged in a first coupled-out beam path, for acquiring a first image and a second image sensor, arranged in a second coupled-out beam path, for acquiring a second image, the autofocus system being configured such that the focusing procedure is executable on the basis of a first operating mode and a second operating mode, the autofocus system being configured such that in the first operating mode the autofocus system ascertains contrast values of the first image acquired by the first image sensor and of the second image acquired by the second image sensor, and in the second operating mode the autofocus system ascertains contrast values of the first image acquired by the first image sensor and of the second image acquired by the second image sensor, and sets a relative location of a focal plane with respect to an object plane based on the ascertained contrast values, the autofocus system being configured to set the relative location of the focal plane with respect to the object plane in the first operating mode such that the focal plane lies within a first tolerance region around the object plane and the relative location of the focal plane with respect to the object plane in the second operating mode such that the focal plane lies within a second tolerance region around the object plane, and the second tolerance region being smaller than the first tolerance region, characterized in that the autofocus system is configured such that the focusing procedure executable based on the first operating mode is carried out only one single time for finding the focus, and the focusing procedure for holding the focus, executable based on the second operating mode, is repeated until a predefined termination condition is satisfied.