An analytical test strip for use with a test meter, the analytical test strip comprising: a first insulating layer with a first insulating layer upper surface; a first electrically conductive layer disposed on the first insulating layer upper surface and including: a first electrode portion; and at least one electrical contact pad in electrical communication with the first electrode portion; a patterned spacer layer disposed above the first electrically conductive layer and including: a distal portion defining a bodily fluid sample-receiving chamber therein that overlies the first electrode portion; and an insulating proximal portion with an upper surface and a second electrically conductive layer disposed thereon, the second electrically conductive layer including: an interlayer contact portion; and an electrical contact pad; a second insulating layer disposed above the patterned spacer layer and having a second insulating layer lower surface; a third electrically conductive layer disposed on the second insulating layer lower surface and including: a second electrode portion; and a proximal portion overlying the interlayer contact portion, wherein the second electrode portion is disposed overlying and exposed to the sample-receiving chamber and in an opposing relationship to the first electrode portion, and wherein the proximal portion of the third electrically conductive layer is operatively juxtaposed with the interlayer contact portion of the second electrically conductive layer such that there is an electrical connection between the second electrode portion of the third electrically conductive layer and the electrical contact pad of the patterned spacer layer during use of the analytical test strip. a first insulating layer with a first insulating layer upper surface; a first electrically conductive layer disposed on the first insulating layer upper surface and including: a first electrode portion; and at least one electrical contact pad in electrical communication with the first electrode portion; a first electrode portion; and at least one electrical contact pad in electrical communication with the first electrode portion; a patterned spacer layer disposed above the first electrically conductive layer and including: a distal portion defining a bodily fluid sample-receiving chamber therein that overlies the first electrode portion; and an insulating proximal portion with an upper surface and a second electrically conductive layer disposed thereon, the second electrically conductive layer including: an interlayer contact portion; and an electrical contact pad; a distal portion defining a bodily fluid sample-receiving chamber therein that overlies the first electrode portion; and an insulating proximal portion with an upper surface and a second electrically conductive layer disposed thereon, the second electrically conductive layer including: an interlayer contact portion; and an electrical contact pad; an interlayer contact portion; and an electrical contact pad; a second insulating layer disposed above the patterned spacer layer and having a second insulating layer lower surface; a third electrically conductive layer disposed on the second insulating layer lower surface and including: a second electrode portion; and a proximal portion overlying the interlayer contact portion, wherein the second electrode portion is disposed overlying and exposed to the sample-receiving chamber and in an opposing relationship to the first electrode portion, and wherein the proximal portion of the third electrically conductive layer is operatively juxtaposed with the interlayer contact portion of the second electrically conductive layer such that there is an electrical connection between the second electrode portion of the third electrically conductive layer and the electrical contact pad of the patterned spacer layer during use of the analytical test strip. a second electrode portion; and a proximal portion overlying the interlayer contact portion,