An AAV multiple vector system to express the coding sequence of a gene of interest in a cell, said coding sequence comprising a first portion and a second portion, said vector system comprising: a) a first vector comprising: - said first portion of said coding sequence, - a first reconstitution sequence; and b) a second vector comprising: - said second portion of said coding sequence, - a second reconstitution sequence, wherein said first and second reconstitution sequences are selected from the group of: i] the first reconstitution sequence consists of the 3' end of said first portion of the coding sequence and the second reconstitution sequence consists of the 5'end of said second portion of the coding sequence, said first and second reconstitution sequences being overlapping sequences; or ii] the first reconstitution sequence comprises a splicing donor signal and the second reconstitution sequence comprises a splicing acceptor signal, optionally each one of first and second reconstitution sequence further comprises a recombinogenic sequence, characterized by the fact that either one or both of the first and second vector further comprises a nucleotide sequence of a degradation signal, said sequence being located in case of i) at the 3' end of the CDS1 and/or at the 5' end of the CDS2 and in case of ii) in 3' position relative to the SD and/or in 5' position relative to the SA, wherein - the recombinogenic sequence is selected from the group consisting of: GGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTT AACGCGAATTTTAACAAAAT (SEQ ID No. 22, AK) or GGGATTTTTCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTT AACGCGAATTTTAACAAAAT (SEQ ID NO. 23, AK), SEQ ID NO. 24, SEQ ID NO. 25, and SEQ ID NO. 26 and - the nucleotide sequence of the degradation signal comprises or consists of a sequence encoding SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, SEQ ID No. 4, SEQ ID No. 5, SEQ ID No. 6, SEQ ID No. 7, SEQ ID No. 8, SEQ ID No. 9, or the nucleotide sequence of a degradation signal comprises or consists of SEQ ID No. 16. a) a first vector comprising: - said first portion of said coding sequence, - a first reconstitution sequence; and - said first portion of said coding sequence, - a first reconstitution sequence; and b) a second vector comprising: - said second portion of said coding sequence, - a second reconstitution sequence, - said second portion of said coding sequence, - a second reconstitution sequence, wherein said first and second reconstitution sequences are selected from the group of: i] the first reconstitution sequence consists of the 3' end of said first portion of the coding sequence and the second reconstitution sequence consists of the 5'end of said second portion of the coding sequence, said first and second reconstitution sequences being overlapping sequences; or ii] the first reconstitution sequence comprises a splicing donor signal and the second reconstitution sequence comprises a splicing acceptor signal, optionally each one of first and second reconstitution sequence further comprises a recombinogenic sequence, i] the first reconstitution sequence consists of the 3' end of said first portion of the coding sequence and the second reconstitution sequence consists of the 5'end of said second portion of the coding sequence, said first and second reconstitution sequences being overlapping sequences; or ii] the first reconstitution sequence comprises a splicing donor signal and the second reconstitution sequence comprises a splicing acceptor signal, optionally each one of first and second reconstitution sequence further comprises a recombinogenic sequence, characterized by the fact that either one or both of the first and second vector further comprises a nucleotide sequence of a degradation signal, said sequence being located in case of i) at the 3' end of the CDS1 and/or at the 5' end of the CDS2 and in case of ii) in 3' position relative to the SD and/or in 5' position relative to the SA, wherein - the recombinogenic sequence is selected from the group consisting of: GGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTT AACGCGAATTTTAACAAAAT (SEQ ID No. 22, AK) or GGGATTTTTCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTT AACGCGAATTTTAACAAAAT (SEQ ID NO. 23, AK), SEQ ID NO. 24, SEQ ID NO. 25, and SEQ ID NO. 26 and - the nucleotide sequence of the degradation signal comprises or consists of a sequence encoding SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, SEQ ID No. 4, SEQ ID No. 5, SEQ ID No. 6, SEQ ID No. 7, SEQ ID No. 8, SEQ ID No. 9, or the nucleotide sequence of a degradation signal comprises or consists of SEQ ID No. 16. - the recombinogenic sequence is selected from the group consisting of: GGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTT AACGCGAATTTTAACAAAAT (SEQ ID No. 22, AK) or GGGATTTTTCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTT AACGCGAATTTTAACAAAAT (SEQ ID NO. 23, AK), SEQ ID NO. 24, SEQ ID NO. 25, and SEQ ID NO. 26 and GGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTT AACGCGAATTTTAACAAAAT (SEQ ID No. 22, AK) or GGGATTTTTCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTT AACGCGAATTTTAACAAAAT (SEQ ID NO. 23, AK), SEQ ID NO. 24, SEQ ID NO. 25, and SEQ ID NO. 26 and - the nucleotide sequence of the degradation signal comprises or consists of a sequence encoding SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, SEQ ID No. 4, SEQ ID No. 5, SEQ ID No. 6, SEQ ID No. 7, SEQ ID No. 8, SEQ ID No. 9, or the nucleotide sequence of a degradation signal comprises or consists of SEQ ID No. 16.