A method of amplifying cytomegalovirus UL34 and UL80.5 nucleic acid sequences in a sample, which method comprises: (a) forming a mixture comprising the sample, nucleic acid amplification reagents, a pair of primers for amplification of a UL34 nucleic acid sequence, and a pair of primers for amplification of a UL80.5 nucleic acid sequence; and (b) subjecting the mixture to conditions that promote amplification of the UL34 nucleic acid sequence and the UL80.5 nucleic acid sequence, whereupon CMV UL34 and UL80.5 nucleic acid sequences in a sample are amplified, wherein: (i) the primers for amplification of a UL34 nucleic acid sequence comprise: 5' TGA ACT TCA TCA TCA CCA CCC GAG ACT 3' [SEQ ID NO: 49] as a forward primer and 5' CCT TGT ACG CTT TGG AAA TCG AGC CTG 3' [SEQ ID NO: 50] as a reverse primer; or (ii) the primers for amplification of a UL34 nucleic acid sequence consist of: 5' TGA ACT TCA TCA TCA CCA CCC GAG ACT 3' [SEQ ID NO: 49] as a forward primer and 5' CCT TGT ACG CTT TGG AAA TCG AGC CTG 3' [SEQ ID NO: 50] as a reverse primer, and wherein: (i) the primers for amplification of a UL80.5 nucleic acid sequence comprise: 5' CGG CTA GTG TCG TGT TAG C 3' [SEQ ID NO: 16] as a forward primer and 5' CAC AAA AAT CCG CCG ATT CAG ATC 3' [SEQ ID NO: 47] as a reverse primer; or (ii) the primers for amplification of a UL80.5 nucleic acid sequence consist of: 5' CGG CTA GTG TCG TGT TAG C 3' [SEQ ID NO: 16] as a forward primer and 5' CAC AAA AAT CCG CCG ATT CAG ATC 3' [SEQ ID NO: 47] as a reverse primer. (a) forming a mixture comprising the sample, nucleic acid amplification reagents, a pair of primers for amplification of a UL34 nucleic acid sequence, and a pair of primers for amplification of a UL80.5 nucleic acid sequence; and (b) subjecting the mixture to conditions that promote amplification of the UL34 nucleic acid sequence and the UL80.5 nucleic acid sequence, (i) the primers for amplification of a UL34 nucleic acid sequence comprise: 5' TGA ACT TCA TCA TCA CCA CCC GAG ACT 3' [SEQ ID NO: 49] as a forward primer and 5' CCT TGT ACG CTT TGG AAA TCG AGC CTG 3' [SEQ ID NO: 50] as a reverse primer; or 5' TGA ACT TCA TCA TCA CCA CCC GAG ACT 3' [SEQ ID NO: 49] as a forward primer and 5' CCT TGT ACG CTT TGG AAA TCG AGC CTG 3' [SEQ ID NO: 50] as a reverse primer; or (ii) the primers for amplification of a UL34 nucleic acid sequence consist of: 5' TGA ACT TCA TCA TCA CCA CCC GAG ACT 3' [SEQ ID NO: 49] as a forward primer and 5' CCT TGT ACG CTT TGG AAA TCG AGC CTG 3' [SEQ ID NO: 50] as a reverse primer, and wherein: (i) the primers for amplification of a UL80.5 nucleic acid sequence comprise: 5' CGG CTA GTG TCG TGT TAG C 3' [SEQ ID NO: 16] as a forward primer and 5' CAC AAA AAT CCG CCG ATT CAG ATC 3' [SEQ ID NO: 47] as a reverse primer; or (ii) the primers for amplification of a UL80.5 nucleic acid sequence consist of: 5' CGG CTA GTG TCG TGT TAG C 3' [SEQ ID NO: 16] as a forward primer and 5' CAC AAA AAT CCG CCG ATT CAG ATC 3' [SEQ ID NO: 47] as a reverse primer. 5' TGA ACT TCA TCA TCA CCA CCC GAG ACT 3' [SEQ ID NO: 49] as a forward primer and 5' CCT TGT ACG CTT TGG AAA TCG AGC CTG 3' [SEQ ID NO: 50] as a reverse primer, and wherein: (i) the primers for amplification of a UL80.5 nucleic acid sequence comprise: 5' CGG CTA GTG TCG TGT TAG C 3' [SEQ ID NO: 16] as a forward primer and 5' CAC AAA AAT CCG CCG ATT CAG ATC 3' [SEQ ID NO: 47] as a reverse primer; or (ii) the primers for amplification of a UL80.5 nucleic acid sequence consist of: 5' CGG CTA GTG TCG TGT TAG C 3' [SEQ ID NO: 16] as a forward primer and (i) the primers for amplification of a UL80.5 nucleic acid sequence comprise: 5' CGG CTA GTG TCG TGT TAG C 3' [SEQ ID NO: 16] as a forward primer and 5' CAC AAA AAT CCG CCG ATT CAG ATC 3' [SEQ ID NO: 47] as a reverse primer; or 5' CGG CTA GTG TCG TGT TAG C 3' [SEQ ID NO: 16] as a forward primer and 5' CAC AAA AAT CCG CCG ATT CAG ATC 3' [SEQ ID NO: 47] as a reverse primer; or (ii) the primers for amplification of a UL80.5 nucleic acid sequence consist of: 5' CGG CTA GTG TCG TGT TAG C 3' [SEQ ID NO: 16] as a forward primer and 5' CAC AAA AAT CCG CCG ATT CAG ATC 3' [SEQ ID NO: 47] as a reverse primer.