A method for detecting the presence of a BRAF V600K mutation in a sample, said method comprising: (a) isolating a nucleic acid from said sample wherein the sample comprises RNA or DNA sequences; (b) performing a first amplification reaction of said RNA or DNA sequences of said sample, wherein said amplification reaction comprises a first primer capable of annealing specifically to a BRAF mutant sequence at a first position in a BRAF RNA or DNA sequence wherein said first primer is SEQ ID NO: 1 and a second primer capable of annealing specifically at a second position in a BRAF RNA or DNA sequence, wherein said first and second primers anneal to different strands of double stranded BRAF RNA or DNA sequence, wherein the amplification reaction is capable of producing a BRAF mutant specific amplification product when the RNA or DNA sequences of the sample comprise a BRAF RNA or DNA sequence comprising a mutant sequence at said first position of said BRAF RNA or DNA sequence; (c) performing a second amplification reaction of said RNA or DNA sequences of said sample, wherein said amplification reaction comprises a first primer wherein said first primer is SEQ ID NO:2 and a second primer capable of annealing specifically at a second position in a BRAF RNA or DNA sequence, wherein due to the stringency of the first primer, the amplification reaction is not capable of producing a BRAF mutant specific amplification product; and (d) visualizing amplification products produced by said first amplification reaction, wherein detection of a BRAF mutant specific amplification product in one sample and lack of amplified product from the second reaction is a positive indicator of a BRAF V600K mutation in said sample, wherein said second primer capable of annealing specifically at a second position in a BRAF nucleic acid sequence is SEQ ID NO: 10. (a) isolating a nucleic acid from said sample wherein the sample comprises RNA or DNA sequences; (b) performing a first amplification reaction of said RNA or DNA sequences of said sample, wherein said amplification reaction comprises a first primer capable of annealing specifically to a BRAF mutant sequence at a first position in a BRAF RNA or DNA sequence wherein said first primer is SEQ ID NO: 1 and a second primer capable of annealing specifically at a second position in a BRAF RNA or DNA sequence, wherein said first and second primers anneal to different strands of double stranded BRAF RNA or DNA sequence, wherein the amplification reaction is capable of producing a BRAF mutant specific amplification product when the RNA or DNA sequences of the sample comprise a BRAF RNA or DNA sequence comprising a mutant sequence at said first position of said BRAF RNA or DNA sequence; (c) performing a second amplification reaction of said RNA or DNA sequences of said sample, wherein said amplification reaction comprises a first primer wherein said first primer is SEQ ID NO:2 and a second primer capable of annealing specifically at a second position in a BRAF RNA or DNA sequence, wherein due to the stringency of the first primer, the amplification reaction is not capable of producing a BRAF mutant specific amplification product; and (d) visualizing amplification products produced by said first amplification reaction, wherein detection of a BRAF mutant specific amplification product in one sample and lack of amplified product from the second reaction is a positive indicator of a BRAF V600K mutation in said sample,