A method of detecting at least one target nucleic acid in a plurality of nucleic acids comprising: a) enrichment of a said at least one target nucleic acid comprising the steps of (i) mixing a sample comprising said at least one target nucleic acid with a nucleic acid probe complementary to at least a portion of the at least one target nucleic acid to form a DNA:RNA hybrid, wherein when said target nucleic acid is RNA, said nucleic acid probe is DNA, and wherein when said target nucleic acid is DNA, said nucleic acid probe is RNA; (ii) capturing said DNA:RNA hybrid onto a solid support; and (iii) removing nucleic acids which do not form said DNA:RNA hybrid or are not captured onto the solid support, thereby forming enriched target nucleic acids; b) amplifying said enriched target nucleic acids from step a), to form a plurality of amplified targets; and c) detecting said at least one target nucleic acid in said plurality of amplified targets by (i) mixing said plurality of amplified targets with a selectable oligonucleotide and a nucleic acid probe, wherein the selectable oligonucleotide hybridizes to a first portion of the amplified targets, and wherein said nucleic acid probe is complementary to a second portion of the amplified targets, wherein when the plurality of amplified targets is RNA, the nucleic acid probe is DNA, and wherein when the plurality of amplified targets is DNA, the nucleic acid probe is RNA, to form a DNA:RNA hybrid oligonucleotide complex, wherein said first portion of the at least one target nucleic acid and said second portion of the target nucleic acid are different; and (ii) detecting said DNA:RNA hybrid oligonucleotide complex by a DNA:RNA hybrid-specific binding agent which is directly or indirectly labelled, thereby detecting said at least one target nucleic acid. a) enrichment of a said at least one target nucleic acid comprising the steps of (i) mixing a sample comprising said at least one target nucleic acid with a nucleic acid probe complementary to at least a portion of the at least one target nucleic acid to form a DNA:RNA hybrid, wherein when said target nucleic acid is RNA, said nucleic acid probe is DNA, and wherein when said target nucleic acid is DNA, said nucleic acid probe is RNA; (ii) capturing said DNA:RNA hybrid onto a solid support; and (iii) removing nucleic acids which do not form said DNA:RNA hybrid or are not captured onto the solid support, thereby forming enriched target nucleic acids; (i) mixing a sample comprising said at least one target nucleic acid with a nucleic acid probe complementary to at least a portion of the at least one target nucleic acid to form a DNA:RNA hybrid, wherein when said target nucleic acid is RNA, said nucleic acid probe is DNA, and wherein when said target nucleic acid is DNA, said nucleic acid probe is RNA; (ii) capturing said DNA:RNA hybrid onto a solid support; and (iii) removing nucleic acids which do not form said DNA:RNA hybrid or are not captured onto the solid support, thereby forming enriched target nucleic acids; b) amplifying said enriched target nucleic acids from step a), to form a plurality of amplified targets; and c) detecting said at least one target nucleic acid in said plurality of amplified targets by (i) mixing said plurality of amplified targets with a selectable oligonucleotide and a nucleic acid probe, wherein the selectable oligonucleotide hybridizes to a first portion of the amplified targets, and wherein said nucleic acid probe is complementary to a second portion of the amplified targets, wherein when the plurality of amplified targets is RNA, the nucleic acid probe is DNA, and wherein when the plurality of amplified targets is DNA, the nucleic acid probe is RNA, to form a DNA:RNA hybrid oligonucleotide complex, wherein said first portion of the at least one target nucleic acid and said second portion of the target nucleic acid are different; and (ii) detecting said DNA:RNA hybrid oligonucleotide complex by a DNA:RNA hybrid-specific binding agent which is directly or indirectly labelled, thereby detecting said at least one target nucleic acid. (i) mixing said plurality of amplified targets with a selectable oligonucleotide and a nucleic acid probe, wherein the selectable oligonucleotide hybridizes to a first portion of the amplified targets, and wherein said nucleic acid probe is complementary to a second portion of the amplified targets, wherein when the plurality of amplified targets is RNA, the nucleic acid probe is DNA, and wherein when the plurality of amplified targets is DNA, the nucleic acid probe is RNA, to form a DNA:RNA hybrid oligonucleotide complex, wherein said first portion of the at least one target nucleic acid and said second portion of the target nucleic acid are different; and (ii) detecting said DNA:RNA hybrid oligonucleotide complex by a DNA:RNA hybrid-specific binding agent which is directly or indirectly labelled, thereby detecting said at least one target nucleic acid.