A method of screening target enzyme activity for production of a phenolic compound using an artificial genetic circuit, the method comprising the steps of: (a) providing an artificial genetic circuit for detecting a phenolic compound, the artificial genetic circuit comprising (i) a gene encoding a regulatory protein, (ii) at least one reporter gene selected from the group consisting of fluorescence protein-encoding genes and antibiotic resistance genes, (iii) a gene expression regulatory region consisting of a promoter regulating the expression of the regulatory protein, a region to which the regulatory protein binds to induce the expression of a downstream reporter gene, and a promoter regulating the expression of the reporter gene, and (iv) RBS (Ribosome Binding Site) for facilitating the expression of the reporter gene and a transcriptional terminator, wherein the regulatory protein of (i) recognizes a phenolic compound to induce binding of the regulatory protein to the gene expression regulatory region and activate a promoter of the reporter gene such that the downstream reporter gene can be expressed; (b) providing a clone or gene library containing a gene encoding the enzyme to be screened; (c) introducing the clone or gene library and the artificial gene circuit for detecting the phenolic compound into host microorganisms to prepare recombinant microorganisms; (d) treating the recombinant microorganisms with a phenol-release compound capable of liberating a phenolic compound by an enzymatic reaction of the target enzyme to be screened; and (e) detecting the activity of the reporter protein whose expression was induced by the regulatory protein sensing the phenolic compound liberated by the enzymatic reaction and binding of the regulatory protein to the gene expression regulatory region. (a) providing an artificial genetic circuit for detecting a phenolic compound, the artificial genetic circuit comprising (i) a gene encoding a regulatory protein, (ii) at least one reporter gene selected from the group consisting of fluorescence protein-encoding genes and antibiotic resistance genes, (iii) a gene expression regulatory region consisting of a promoter regulating the expression of the regulatory protein, a region to which the regulatory protein binds to induce the expression of a downstream reporter gene, and a promoter regulating the expression of the reporter gene, and (iv) RBS (Ribosome Binding Site) for facilitating the expression of the reporter gene and a transcriptional terminator, wherein the regulatory protein of (i) recognizes a phenolic compound to induce binding of the regulatory protein to the gene expression regulatory region and activate a promoter of the reporter gene such that the downstream reporter gene can be expressed; (b) providing a clone or gene library containing a gene encoding the enzyme to be screened; (c) introducing the clone or gene library and the artificial gene circuit for detecting the phenolic compound into host microorganisms to prepare recombinant microorganisms; (d) treating the recombinant microorganisms with a phenol-release compound capable of liberating a phenolic compound by an enzymatic reaction of the target enzyme to be screened; and (e) detecting the activity of the reporter protein whose expression was induced by the regulatory protein sensing the phenolic compound liberated by the enzymatic reaction and binding of the regulatory protein to the gene expression regulatory region.