A method of producing a biomatrix scaffold from biological tissue selected from the group consisting of lung, thyroid, skin, blood vessels, bladder, kidneys, brain, abdominal aorta, iliac vein, heart, or intestines for industrial scale dispersal onto culture apparatus, comprising: a) perfusing the biological tissue or homogenizing the biological tissue with a buffer comprising a salt concentration from about 3.5M NaCl to about 4.5M NaCl; then b) perfusing the biological tissue or extracting the homogenate of step (a) with a delipidating buffer comprising phospholipase A2 and sodium deoxycholate in a first medium, wherein the osmolality of said first medium is from about 250 mOsm/kg to about 350 mOsm/kg and said first medium is serum free and at neutral pH; then c) perfusing the tissue or extracting the homogenate of step (b) with a buffer at a neutral pH and comprising a salt concentration from about 2.0M NaCl to about 5.0M NaCl, the concentration chosen to keep insoluble collagens identified in the biological tissue; then d) perfusing the tissue or extracting the homogenate of step (c) with RNase and DNase in a buffer; and then e) rinsing the tissue or homogenate of step (d) with a second medium that is at neutral pH, is serum-free and has an osomolality from about 250 mOsm/kg to about 350 mOsm/kg, thereby producing an intact or homogenized biomatrix scaffold from the biological tissue, said biomatrix scaffold comprising at least 95% of the collagens and most collagen-associated matrix components and matrix bound growth factors, hormones and cytokines of the biological tissue; f) diluting the biomatrix scaffold in basal medium; g) freezing the biomatrix scaffold of (f) at about -80°C; h) pulverizing the biomatrix scaffold of (g) by cryogenic grinding into biomatrix particles ranging in size from about 1 μιη to about 100 μιη; i) thawing the biomatrix particles of (h) in suspension in basal medium; and j) dispersing the biomatrix particles of step (i) onto a culture apparatus, thereby producing a biomatrix scaffold from biological tissue for industrial scale dispersal onto culture apparatus. a) perfusing the biological tissue or homogenizing the biological tissue with a buffer comprising a salt concentration from about 3.5M NaCl to about 4.5M NaCl; then b) perfusing the biological tissue or extracting the homogenate of step (a) with a delipidating buffer comprising phospholipase A2 and sodium deoxycholate in a first medium, wherein the osmolality of said first medium is from about 250 mOsm/kg to about 350 mOsm/kg and said first medium is serum free and at neutral pH; then c) perfusing the tissue or extracting the homogenate of step (b) with a buffer at a neutral pH and comprising a salt concentration from about 2.0M NaCl to about 5.0M NaCl, the concentration chosen to keep insoluble collagens identified in the biological tissue; then d) perfusing the tissue or extracting the homogenate of step (c) with RNase and DNase in a buffer; and then e) rinsing the tissue or homogenate of step (d) with a second medium that is at neutral pH, is serum-free and has an osomolality from about 250 mOsm/kg to about 350 mOsm/kg, thereby producing an intact or homogenized biomatrix scaffold from the biological tissue, said biomatrix scaffold comprising at least 95% of the collagens and most collagen-associated matrix components and matrix bound growth factors, hormones and cytokines of the biological tissue; f) diluting the biomatrix scaffold in basal medium; g) freezing the biomatrix scaffold of (f) at about -80°C; h) pulverizing the biomatrix scaffold of (g) by cryogenic grinding into biomatrix particles ranging in size from about 1 μιη to about 100 μιη; i) thawing the biomatrix particles of (h) in suspension in basal medium; and j) dispersing the biomatrix particles of step (i) onto a culture apparatus,