Manufacturing procedure for a cell- and protein-factor-free collagen matrix or membrane with antimicrobial properties, CHARACTERIZED in that it consists of the following steps: i) forming collagen from fetal amniotic membranes; ii) adding and incorporating metallic nanoparticles to the thus formed collagen in order to form the collagen matrix or membrane with antimicrobial properties; and iii) dehydrating and irradiating the collagen matrix or membrane with antimicrobial properties in order to form a ready-to-pack product; and in that, for the collagen to be formed from the fetal amniotic membrane, this is treated chemically in order to remove any protein rest that might remain therein in a first treatment comprising dipping the fetal amniotic membrane in a 0.01 % sodium dodecyl sulfate solution for one hour at room temperature, in agitation by using an orbital shaker and inside a tightly closed container, and when the first treatment is finished, a second chemical treatment is performed in order to remove any cell that might remain attached to the fetal amniotic membrane, for which the fetal amniotic membrane is dipped in a pH marker-free Trypsin-EDTA solution (colorless) for 1 hour at 37 °C; then, three washes are then performed with physiological saline for 15 minutes, each one to remove any cell that might have detached from the tissue during the trypsin wash process, and when the second treatment is finished, a new chemical treatment is performed with 0.01 % SDS for one hour at room temperature, in agitation by using an orbital shaker and inside a closed container, for the collagen to finally form from amniotic membrane, and in that for metallic nanoparticles to be added and incorporated into the formed collagen, the collagen formed is embedded in an aqueous solution containing the metallic nanoparticles, and it is placed in an electrophoresis chamber and submitted to an electric field of 50 Volts for 30 min for the nanoparticles to be incorporated into the formed collagen and spread evenly. i) forming collagen from fetal amniotic membranes; ii) adding and incorporating metallic nanoparticles to the thus formed collagen in order to form the collagen matrix or membrane with antimicrobial properties; and iii) dehydrating and irradiating the collagen matrix or membrane with antimicrobial properties in order to form a ready-to-pack product; when the first treatment is finished, a second chemical treatment is performed in order to remove any cell that might remain attached to the fetal amniotic membrane, for which the fetal amniotic membrane is dipped in a pH marker-free Trypsin-EDTA solution (colorless) for 1 hour at 37 °C; then, three washes are then performed with physiological saline for 15 minutes, each one to remove any cell that might have detached from the tissue during the trypsin wash process, and when the second treatment is finished, a new chemical treatment is performed with 0.01 % SDS for one hour at room temperature, in agitation by using an orbital shaker and inside a closed container, for the collagen to finally form from amniotic membrane, and in that for metallic nanoparticles to be added and incorporated into the formed collagen, the collagen formed is embedded in an aqueous solution containing the metallic nanoparticles, and it is placed in an electrophoresis chamber and submitted to an electric field of 50 Volts for 30 min for the nanoparticles to be incorporated into the formed collagen and spread evenly.