A synthetic culture media for the isolation and maintenance of Terfezia spp. comprising: - sucrose and/or glucose as carbon source at a concentration in the range of 5 to 15 (g/L), preferably of 7.5 to 15 (g/L), even more preferably of 10 (g/L), the following macronutrients: - KNO 3 at a concentration of 0.40 and 0.55 (g/L), preferably of 0.475 (g/L), - NH 4 NO 3 at a concentration of 0.35 and 0.48 (g/L), preferably of 0.413 (g/L), - CaCl 2.2H 2 O at a concentration of 0.093 and 0.13 (g/L), preferably of 0.11 (g/L), - MgSO 4.7H 2 O at a concentration of 0.093 and 0.106 (g/L), preferably of 0.079 (g/L), - KH 2 PO 4 at a concentration of 0.036 and 0.049 (g/L), preferably of 0.043 (g/L), the following micronutrients: - iron chelates Na 2 EDTA at a concentration in the range of 0.0079 to 0.0107 (g/L), preferably of 0.0093 (g/L), and FeSO 4.7H 2 O in the range of 0.0059 to 0.0080 (g/L), preferably of 0.0070 (g/L); - MnSO 4.4H 2 O at a concentration of 0.0036 and 0.0049 (g/L), preferably of 0.0042 (g/L); - ZnSO 4.7H 2 O at a concentration of 0.0018 and 0.0025 (g/L), preferably of 0.0022 (g/L); - CuSO 4.5H 2 O at a concentration of 5.1×10 -6 and 6.9×10 -6 (g/L), preferably of 6.0×10 -6 (g/L); - CoCl 2.6H 2 O at a concentration of 5.1×10 -6 and 6.9×10 -6 (g/L), preferably of 6.0×10 -6 (g/L); - Na 2 MoO 4.2H 2 O at a concentration of 5.1×10 -6 and 6.9×10 -6 (g/L), preferably of 6.0×10 -6 (g/L); - H 3 BO 3 at a concentration of 0.0013 and 0.0018 (g/L), preferably of 0.0016 (g/L), and - KI at a concentration of 0.00018 and 0.00024 (g/L), preferably of 0.0016 (g/L), the following vitamins and growth factors: - thiamine hydrochloride at a concentration of 2.1×10 -5 and 2.9×10 -5 (g/L), preferably of 2.5×10 -5 (g/L), - nicotinic acid at a concentration of 0.00011 and 0.00014 (g/L), preferably of 0.00013 (g/L), - pyridoxine hydrochloride at a concentration of 0.00011 and 0.00014 (g/L), preferably of 0.00013 (g/L), - myo-Inositol at a concentration of 0.021 and 0.029 (g/L), preferably of 0.025 (g/L), and - glycine at a concentration of 0.00043 and 0.00058 (g/L), preferably of 0.00050 (g/L), and the following phytohormones: - a cytokinin of the adenine-type the 6-benzylaminopurine at a concentration in the range of 0.0004 to 0.0006 (g/L), preferably of 0.0005 (g/L), and - an auxin indole-3-acetic acid, in a concentration in the range of 0.0004 to 0.0006 (g/L), preferably of 0.0005 (g/L). - sucrose and/or glucose as carbon source at a concentration in the range of 5 to 15 (g/L), preferably of 7.5 to 15 (g/L), even more preferably of 10 (g/L), - KNO 3 at a concentration of 0.40 and 0.55 (g/L), preferably of 0.475 (g/L), - NH 4 NO 3 at a concentration of 0.35 and 0.48 (g/L), preferably of 0.413 (g/L), - CaCl 2.2H 2 O at a concentration of 0.093 and 0.13 (g/L), preferably of 0.11 (g/L), - MgSO 4.7H 2 O at a concentration of 0.093 and 0.106 (g/L), preferably of 0.079 (g/L), - KH 2 PO 4 at a concentration of 0.036 and 0.049 (g/L), preferably of 0.043 (g/L), - iron chelates Na 2 EDTA at a concentration in the range of 0.0079 to 0.0107 (g/L), preferably of 0.0093 (g/L), and FeSO 4.7H 2 O in the range of 0.0059 to 0.0080 (g/L), preferably of 0.0070 (g/L); - MnSO 4.4H 2 O at a concentration of 0.0036 and 0.0049 (g/L), preferably of 0.0042 (g/L); - ZnSO 4.7H 2 O at a concentration of 0.0018 and 0.0025 (g/L), preferably of 0.0022 (g/L); - CuSO 4.5H 2 O at a concentration of 5.1×10 -6 and 6.9×10 -6 (g/L), preferably of 6.0×10 -6 (g/L); - CoCl 2.6H 2 O at a concentration of 5.1×10 -6 and 6.9×10 -6 (g/L), preferably of 6.0×10 -6 (g/L); - Na 2 MoO 4.2H 2 O at a concentration of 5.1×10 -6 and 6.9×10 -6 (g/L), preferably of 6.0×10 -6 (g/L); - H 3 BO 3 at a concentration of 0.0013 and 0.0018 (g/L), preferably of 0.0016 (g/L), and - KI at a concentration of 0.00018 and 0.00024 (g/L), preferably of 0.0016 (g/L), - thiamine hydrochloride at a concentration of 2.1×10 -5 and 2.9×10 -5 (g/L), preferably of 2.5×10 -5 (g/L), - nicotinic acid at a concentration of 0.00011 and 0.00014 (g/L), preferably of 0.00013 (g/L), - pyridoxine hydrochloride at a concentration of 0.00011 and 0.00014 (g/L), preferably of 0.00013 (g/L), - myo-Inositol at a concentration of 0.021 and 0.029 (g/L), preferably of 0.025 (g/L), and - glycine at a concentration of 0.00043 and 0.00058 (g/L), preferably of 0.00050 (g/L), - a cytokinin of the adenine-type the 6-benzylaminopurine at a concentration in the range of 0.0004 to 0.0006 (g/L), preferably of 0.0005 (g/L), and - an auxin indole-3-acetic acid, in a concentration in the range of 0.0004 to 0.0006 (g/L), preferably of 0.0005 (g/L).