A method for generating an image of a sample by means of microscopy methods which provide different spatial resolutions, wherein at least two of the following microscopy methods are combined: - a first microscopy method in which the sample is excited to luminescence by structured line-type or wide-field illumination, the structuring is rotated and shifted several times for each rotation position, wherein at least three rotation positions and at least three shift positions per rotation position are realized, in each position the luminescent sample is imaged onto a 2D detector with a predetermined optical resolution and a first microscopic image with spatial resolution increased beyond the predetermined optical resolution is generated from the thus-obtained frames by a computational processing comprising Fourier analysis, - a second microscopy method in which the sample is labelled with label molecules which can be excited to emit particular luminescence radiation only after being activated by a switching signal, the switching signal is applied to the sample such that only a sub-set of the label molecules present in the sample is activated, wherein there are sub-areas in the sample in which activated label molecules have a distance to their closest neighbouring activated label molecules which is greater than or equal to the predetermined optical resolution, the activated molecules are excited to emit luminescence radiation, the sample emitting luminescence radiation is imaged onto the 2D detector with the predetermined optical resolution, the image is analyzed and image data are generated from it which give the geometric locations of the label molecules emitting luminescence radiation with a local resolution increased above the optical resolution, and a second microscopic image is generated from the image data, - a third microscopy method for generating a third microscopic image by means of laser scanning microscopy, - wherein the at least two obtained microscopic images are combined into a composite image, in particular are superimposed. - a first microscopy method in which the sample is excited to luminescence by structured line-type or wide-field illumination, the structuring is rotated and shifted several times for each rotation position, wherein at least three rotation positions and at least three shift positions per rotation position are realized, in each position the luminescent sample is imaged onto a 2D detector with a predetermined optical resolution and a first microscopic image with spatial resolution increased beyond the predetermined optical resolution is generated from the thus-obtained frames by a computational processing comprising Fourier analysis, - a second microscopy method in which the sample is labelled with label molecules which can be excited to emit particular luminescence radiation only after being activated by a switching signal, the switching signal is applied to the sample such that only a sub-set of the label molecules present in the sample is activated, wherein there are sub-areas in the sample in which activated label molecules have a distance to their closest neighbouring activated label molecules which is greater than or equal to the predetermined optical resolution, the activated molecules are excited to emit luminescence radiation, the sample emitting luminescence radiation is imaged onto the 2D detector with the predetermined optical resolution, the image is analyzed and image data are generated from it which give the geometric locations of the label molecules emitting luminescence radiation with a local resolution increased above the optical resolution, and a second microscopic image is generated from the image data, - a third microscopy method for generating a third microscopic image by means of laser scanning microscopy, - wherein the at least two obtained microscopic images are combined into a composite image, in particular are superimposed.