A display substrate, having a plurality of sub-pixels arranged in a plurality of rows and columns, and comprising a base substrate, a driving circuit layer on the base substrate, a light-emitting device layer on a side of the driving circuit layer away from the base substrate, and a black matrix layer on a side of the light-emitting device layer away from the base substrate, wherein each of the plurality of sub-pixels comprises a pixel driving circuit in the driving circuit layer and a light-emitting device in the light-emitting device layer, and the pixel driving circuit is configured to drive the light-emitting device; the driving circuit layer comprises a first signal line and a second signal line arranged parallel to each other and arranged periodically, and the first signal line and the second signal line are configured to provide different electrical signals to the plurality of sub-pixels; the black matrix layer comprises a plurality of first light-transmitting openings and a plurality of second light-transmitting openings, the plurality of first light-transmitting openings respectively expose light-emitting devices of the plurality of sub-pixels, and the plurality of second light-transmitting openings are respectively arranged between the plurality of first light-transmitting openings; and wherein orthographic projections of the plurality of second light-transmitting openings on the base substrate are respectively between an orthographic projection of one first signal line on the base substrate and an orthographic projection of one second signal line closest to the one first signal line on the base substrate; characterized in that the driving circuit layer comprises a third signal line and a fourth signal line arranged parallel to each other and arranged periodically, the third signal line and the fourth signal line respectively intersects the first signal line and the second signal line, and the third signal line and the fourth signal line are configured to provide different electrical signals to the plurality of sub-pixels; and the orthographic projections of the plurality of second light-transmitting openings on the base substrate are respectively between an orthographic projection of one third signal line on the base substrate and an orthographic projection of one fourth signal line adjacent to the one third signal line on the base substrate. wherein each of the plurality of sub-pixels comprises a pixel driving circuit in the driving circuit layer and a light-emitting device in the light-emitting device layer, and the pixel driving circuit is configured to drive the light-emitting device; the driving circuit layer comprises a first signal line and a second signal line arranged parallel to each other and arranged periodically, and the first signal line and the second signal line are configured to provide different electrical signals to the plurality of sub-pixels; the black matrix layer comprises a plurality of first light-transmitting openings and a plurality of second light-transmitting openings, the plurality of first light-transmitting openings respectively expose light-emitting devices of the plurality of sub-pixels, and the plurality of second light-transmitting openings are respectively arranged between the plurality of first light-transmitting openings; and wherein orthographic projections of the plurality of second light-transmitting openings on the base substrate are respectively between an orthographic projection of one first signal line on the base substrate and an orthographic projection of one second signal line closest to the one first signal line on the base substrate; characterized in that the driving circuit layer comprises a third signal line and a fourth signal line arranged parallel to each other and arranged periodically, the third signal line and the fourth signal line respectively intersects the first signal line and the second signal line, and the third signal line and the fourth signal line are configured to provide different electrical signals to the plurality of sub-pixels; and the orthographic projections of the plurality of second light-transmitting openings on the base substrate are respectively between an orthographic projection of one third signal line on the base substrate and an orthographic projection of one fourth signal line adjacent to the one third signal line on the base substrate.