A method of producing a biomatrix scaffold from mammalian liver tissue for industrial scale dispersal onto culture apparatus, comprising: a) perfusing the liver tissue or homogenizing the liver tissue with a buffer comprising a salt concentration from 3.5M NaCl to 4.5M NaCl, then b) perfusing the liver tissue or extracting the homogenate of step (a) with a delipidating buffer comprising sodium deoxycholate and phospholipase A2 in a first medium, wherein the osmolality of said first medium is from 250 mOsm/kg to 350 mOsm/kg and said first medium is serum free and at neutral pH; then c) perfusing the tissue or extracting the homogenate of step (b) with a buffer at a neutral pH and comprising a salt concentration from 2.0M NaCl to 5.0M NaCl, the concentration chosen to keep insoluble collagens identified in the biological tissue; then d) perfusing the tissue or extracting the homogenate of step (c) with RNase and DNase in a buffer; and then e) rinsing the tissue or homogenate of step (d) with a second medium that is at neutral pH, is serum-free and has an osomolality from 250 mOsm/kg to 350 mOsm/kg, thereby producing an intact or homogenized biomatrix scaffold from the liver tissue, said biomatrix scaffold retaining at least 95% of its original collagens and most collagen-associated matrix components and matrix bound growth factors, hormones and cytokines of the biological tissue; f) diluting the biomatrix scaffold in basal medium; g) freezing the biomatrix scaffold of (f); h) pulverizing the biomatrix scaffold of (g) by cryogenic grinding into biomatrix particles ranging in size from 1 μm to 100 μm; i) thawing the biomatrix particles of (h) in suspension in basal medium; and j) dispersing the biomatrix particles of step (i) onto a culture apparatus, thereby producing a biomatrix scaffold from biological tissue for industrial scale dispersal onto culture apparatus. a) perfusing the liver tissue or homogenizing the liver tissue with a buffer comprising a salt concentration from 3.5M NaCl to 4.5M NaCl, then b) perfusing the liver tissue or extracting the homogenate of step (a) with a delipidating buffer comprising sodium deoxycholate and phospholipase A2 in a first medium, wherein the osmolality of said first medium is from 250 mOsm/kg to 350 mOsm/kg and said first medium is serum free and at neutral pH; then c) perfusing the tissue or extracting the homogenate of step (b) with a buffer at a neutral pH and comprising a salt concentration from 2.0M NaCl to 5.0M NaCl, the concentration chosen to keep insoluble collagens identified in the biological tissue; then d) perfusing the tissue or extracting the homogenate of step (c) with RNase and DNase in a buffer; and then e) rinsing the tissue or homogenate of step (d) with a second medium that is at neutral pH, is serum-free and has an osomolality from 250 mOsm/kg to 350 mOsm/kg, thereby producing an intact or homogenized biomatrix scaffold from the liver tissue, said biomatrix scaffold retaining at least 95% of its original collagens and most collagen-associated matrix components and matrix bound growth factors, hormones and cytokines of the biological tissue; f) diluting the biomatrix scaffold in basal medium; g) freezing the biomatrix scaffold of (f); h) pulverizing the biomatrix scaffold of (g) by cryogenic grinding into biomatrix particles ranging in size from 1 μm to 100 μm; i) thawing the biomatrix particles of (h) in suspension in basal medium; and j) dispersing the biomatrix particles of step (i) onto a culture apparatus, thereby producing a biomatrix scaffold from biological tissue for industrial scale dispersal onto culture apparatus.