A negative electrode for a lithium secondary battery, comprising: a negative electrode current collector layer; a first negative electrode active material layer on one surface or both surfaces of the negative electrode current collector layer; and a second negative electrode active material layer on a surface opposite to a surface of the first negative electrode active material layer facing the negative electrode current collector layer, wherein the first negative electrode active material layer comprises a first negative electrode active material layer composition comprising a first negative electrode active material, and the second negative electrode active material layer comprises a second negative electrode active material layer composition comprising a second negative electrode active material, the first negative electrode active material comprises one or more selected from the group consisting of SiOx, wherein x=0 and SiOx, wherein 0<x<2, and comprises 95 parts by weight or more of the SiOx, wherein x=0 based on 100 parts by weight of the first negative electrode active material, the second negative electrode active material comprises one or more selected from the group consisting of a carbon-based active material, a silicon-based active material, a metal-based active material capable of forming an alloy with lithium and a lithium-containing nitride, the silicon-based active material is present in an amount of 1 part by weight or more and 100 parts by weight or less based on 100 parts by weight of the second negative electrode active material, and the second negative electrode active material layer satisfies the following Equation 1: 0.5 ≤ B / A ≤ 2 in Equation 1, A is a discharge capacity of the second negative electrode active material layer, and B means a capacity of pre-lithiation lithium. a negative electrode current collector layer; a first negative electrode active material layer on one surface or both surfaces of the negative electrode current collector layer; and a second negative electrode active material layer on a surface opposite to a surface of the first negative electrode active material layer facing the negative electrode current collector layer, wherein the first negative electrode active material layer comprises a first negative electrode active material layer composition comprising a first negative electrode active material, and the second negative electrode active material layer comprises a second negative electrode active material layer composition comprising a second negative electrode active material, the first negative electrode active material comprises one or more selected from the group consisting of SiOx, wherein x=0 and SiOx, wherein 0<x<2, and comprises 95 parts by weight or more of the SiOx, wherein x=0 based on 100 parts by weight of the first negative electrode active material, the second negative electrode active material comprises one or more selected from the group consisting of a carbon-based active material, a silicon-based active material, a metal-based active material capable of forming an alloy with lithium and a lithium-containing nitride, the silicon-based active material is present in an amount of 1 part by weight or more and 100 parts by weight or less based on 100 parts by weight of the second negative electrode active material, and the second negative electrode active material layer satisfies the following Equation 1: 0.5 ≤ B / A ≤ 2 in Equation 1, A is a discharge capacity of the second negative electrode active material layer, and B means a capacity of pre-lithiation lithium. A is a discharge capacity of the second negative electrode active material layer, and B means a capacity of pre-lithiation lithium.