An inline buffer dilution system, comprising: a first flow control valve adapted to communicate with a vessel comprising a supply of diluent liquid; a second flow control valve adapted to communicate with a first vessel comprising a supply of a first buffer; a third flow control valve adapted to communicate with a second vessel comprising a supply of a second buffer; a mixing pump fluidly connected to the first, second, and third flow control valves (104, 108, 112) and configured to receive an amount of the diluent liquid, the first buffer, and the second buffer via the first, second, and third flow control valves (104, 108, 112), respectively, the mixing pump further configured to mix the amount of the diluent liquid, the first buffer, and the second buffer to produce a diluted buffer solution; a backpressure control valve arranged downstream of the mixing pump and configured to generate a backpressure that promotes mixing within the mixing pump; and a controller communicatively connected to the mixing pump and the backpressure control valve, the controller configured to control the backpressure generated by the backpressure control valve based on the amount of the diluent liquid, the first buffer, and the second buffer being mixed in the mixing pump, such that the mixing pump yields a minimum mixing threshold across a range of fluid flows, wherein the controller is configured to restrict a flow of the diluent liquid through the first flow control valve, thereby creating a negative pressure upstream of the mixing pump that drives the supply of the first buffer solution and the supply of the second buffer solution through the second flow control valve and the third flow control valve (108, 112), respectively, and to the mixing pump. a first flow control valve adapted to communicate with a vessel comprising a supply of diluent liquid; a second flow control valve adapted to communicate with a first vessel comprising a supply of a first buffer; a third flow control valve adapted to communicate with a second vessel comprising a supply of a second buffer; a mixing pump fluidly connected to the first, second, and third flow control valves (104, 108, 112) and configured to receive an amount of the diluent liquid, the first buffer, and the second buffer via the first, second, and third flow control valves (104, 108, 112), respectively, the mixing pump further configured to mix the amount of the diluent liquid, the first buffer, and the second buffer to produce a diluted buffer solution; a backpressure control valve arranged downstream of the mixing pump and configured to generate a backpressure that promotes mixing within the mixing pump; and a controller communicatively connected to the mixing pump and the backpressure control valve, the controller configured to control the backpressure generated by the backpressure control valve based on the amount of the diluent liquid, the first buffer, and the second buffer being mixed in the mixing pump, such that the mixing pump yields a minimum mixing threshold across a range of fluid flows, wherein the controller is configured to restrict a flow of the diluent liquid through the first flow control valve, thereby creating a negative pressure upstream of the mixing pump that drives the supply of the first buffer solution and the supply of the second buffer solution through the second flow control valve and the third flow control valve (108, 112), respectively, and to the mixing pump.