An amadoriase selected from the group consisting of the following: (i) an amadoriase comprising an amino acid sequence in which, when aligned with the amino acid sequence as shown in SEQ ID NO: 1, the amino acid at the position corresponding to position 62 of the amino acid sequence as shown in SEQ ID NO: 1 is alanine, asparagine or aspartic acid and having activity on α-fructosyl hexapeptide (αF6P) wherein said amadoriase exhibits 90% or higher sequence identity with the amino acid sequence as shown in SEQ ID NO:1 over the full length; (ii) an amadoriase comprising the amino acid sequence as shown in SEQ ID NO:1, wherein the amino acid at position 62 of the amino acid sequence as shown in SEQ ID NO:1 is alanine, asparagine or aspartic acid and having activity on α-fructosyl hexapeptide (aF6P), said amadoriase consisting of an amino acid sequence having a substitution, deletion, or addition of one to 15 amino acids at positions other than position 62 in the amino acid sequence as shown in SEQ ID NO: 1 and having activity on aF6P;and (iii) an amadoriase comprising an amino acid sequence in which the amino acid at the position corresponding to position 62 in the amino acid sequence as shown in SEQ ID NO: 1 is alanine, asparagine or aspartic acid, wherein said amadoriase exhibits 70% or higher sequence identity with the amino acid sequence as shown in SEQ ID NO: 1 over the full length and 90% or higher sequence identity with the amino acid sequence as shown in SEQ ID NO: 1 in the homologous region consisting of amino acids of positions 10 to 32, 36 to 41, 49 to 52, 54 to 58, 63 to 65, 73 to 75, 84 to 86, 88 to 90, 120 to 122, 145 to 150, 156 to 162, 164 to 170, 180 to 182, 202 to 205, 207 to 211, 214 to 224, 227 to 230, 236 to 241, 243 to 248, 258 to 261, 266 to 268, 270 to 273, 275 to 287, 295 to 297, 306 to 308, 310 to 316, 324 to 329, 332 to 334, 341 to 344, 346 to 355, 357 to 363, 370 to 383, 385 to 387, 389 to 394, 405 to 410, and 423 to 431, and wherein said amadoriase has activity on aF6P. (i) an amadoriase comprising an amino acid sequence in which, when aligned with the amino acid sequence as shown in SEQ ID NO: 1, the amino acid at the position corresponding to position 62 of the amino acid sequence as shown in SEQ ID NO: 1 is alanine, asparagine or aspartic acid and having activity on α-fructosyl hexapeptide (αF6P) wherein said amadoriase exhibits 90% or higher sequence identity with the amino acid sequence as shown in SEQ ID NO:1 over the full length; (ii) an amadoriase comprising the amino acid sequence as shown in SEQ ID NO:1, wherein the amino acid at position 62 of the amino acid sequence as shown in SEQ ID NO:1 is alanine, asparagine or aspartic acid and having activity on α-fructosyl hexapeptide (aF6P), said amadoriase consisting of an amino acid sequence having a substitution, deletion, or addition of one to 15 amino acids at positions other than position 62 in the amino acid sequence as shown in SEQ ID NO: 1 and having activity on aF6P;and (iii) an amadoriase comprising an amino acid sequence in which the amino acid at the position corresponding to position 62 in the amino acid sequence as shown in SEQ ID NO: 1 is alanine, asparagine or aspartic acid, wherein said amadoriase exhibits 70% or higher sequence identity with the amino acid sequence as shown in SEQ ID NO: 1 over the full length and 90% or higher sequence identity with the amino acid sequence as shown in SEQ ID NO: 1 in the homologous region consisting of amino acids of positions 10 to 32, 36 to 41, 49 to 52, 54 to 58, 63 to 65, 73 to 75, 84 to 86, 88 to 90, 120 to 122, 145 to 150, 156 to 162, 164 to 170, 180 to 182, 202 to 205, 207 to 211, 214 to 224, 227 to 230, 236 to 241, 243 to 248, 258 to 261, 266 to 268, 270 to 273, 275 to 287, 295 to 297, 306 to 308, 310 to 316, 324 to 329, 332 to 334, 341 to 344, 346 to 355, 357 to 363, 370 to 383, 385 to 387, 389 to 394, 405 to 410, and 423 to 431, and wherein said amadoriase has activity on aF6P.