A chromatographic process for the enrichment of at least one compound of interest from a mixture comprising said at least one compound of interest as well as at least one further compound (W, C, S), which is not of interest, using at least two chromatographic columns, wherein said process at least involves a sequence of the following steps: (i) a cyclic accumulation phase, in which the at least two chromatographic columns are alternatingly or synchronously operated in an interconnected phase, followed by a disconnected phase, wherein after these two phases the first and second column or group of columns exchange positions to undergo the next interconnected and disconnected phases of the cyclic accumulation phase; wherein at least one of said columns or group of columns in at least one said interconnected phases or disconnected phases is fed with mixture via its inlet; wherein said two phases of the cyclic accumulation phase are carried out sequentially M times with M > 1; (ii) a cyclic separation phase, in which the at least two chromatographic columns are alternatingly or synchronously operated in an interconnected phase, followed by a disconnected phase, wherein after these two phases forming a separation cycle the first and second column or group of columns exchange positions to undergo the next interconnected and disconnected phases of the cyclic separation phase; wherein via the outlet of at least one of said columns or group of columns the at least one further compound (W, C, S), which is not of interest, is discharged from the system; wherein said two phases of the cyclic separation phase are carried out sequentially N times with N ≥ 0; (iii) an elution phase, in which from the column or group of columns, which at the end of phase (i) or, in case of N>0 at the end of phase (ii) contains the at least one compound of interest, said at least one compound of interest is extracted via the outlet. (i) a cyclic accumulation phase, in which the at least two chromatographic columns are alternatingly or synchronously operated in an interconnected phase, followed by a disconnected phase, wherein after these two phases the first and second column or group of columns exchange positions to undergo the next interconnected and disconnected phases of the cyclic accumulation phase; wherein at least one of said columns or group of columns in at least one said interconnected phases or disconnected phases is fed with mixture via its inlet; wherein said two phases of the cyclic accumulation phase are carried out sequentially M times with M > 1; (ii) a cyclic separation phase, in which the at least two chromatographic columns are alternatingly or synchronously operated in an interconnected phase, followed by a disconnected phase, wherein after these two phases forming a separation cycle the first and second column or group of columns exchange positions to undergo the next interconnected and disconnected phases of the cyclic separation phase; wherein via the outlet of at least one of said columns or group of columns the at least one further compound (W, C, S), which is not of interest, is discharged from the system; wherein said two phases of the cyclic separation phase are carried out sequentially N times with N ≥ 0; (iii) an elution phase, in which from the column or group of columns, which at the end of phase (i) or, in case of N>0 at the end of phase (ii) contains the at least one compound of interest, said at least one compound of interest is extracted via the outlet.