mhurhangee/ep-ind-claims huggingface dataset:
A method for assembling a target nucleic acid, the method comprising: (a) providing a plurality of double-stranded polynucleotides, each polynucleotide comprising a flanking region on the 3' end, or 5' end, or 3' end and 5' end, wherein the flanking region comprises a first recognition site and a second recognition site, wherein the plurality of double-stranded polynucleotides together comprise the target nucleic acid; (b) nicking, in vitro, a first strand of each polynucleotide with a first nickase to produce a first nick, wherein the first nickase is configured to recognize and bind to the first recognition site; and (c) nicking, in vitro, a second strand of each polynucleotide with a second nickase to produce a second nick, wherein the second nickase is configured to recognize and bind to the second recognition site, thereby producing cleaved polynucleotide fragments, each having an overhang defined by the first nick and the second nick, wherein the overhang is predesigned by selecting the first and second recognition sites, and (d) assembling the cleaved polynucleotide fragments at the overhangs to form the target nucleic acid, wherein a first overhang of a first cleaved polynucleotide fragment is complementary to a second overhang of a second cleaved polynucleotide fragment, and wherein said assembling comprises ligating the assembled cleaved polynucleotide fragments to produce a ligated product, wherein at least one of the plurality of double-stranded polynucleotides was provided on a solid support. (a) providing a plurality of double-stranded polynucleotides, each polynucleotide comprising a flanking region on the 3' end, or 5' end, or 3' end and 5' end, wherein the flanking region comprises a first recognition site and a second recognition site, wherein the plurality of double-stranded polynucleotides together comprise the target nucleic acid; (b) nicking, in vitro, a first strand of each polynucleotide with a first nickase to produce a first nick, wherein the first nickase is configured to recognize and bind to the first recognition site; and (c) nicking, in vitro, a second strand of each polynucleotide with a second nickase to produce a second nick, wherein the second nickase is configured to recognize and bind to the second recognition site, thereby producing cleaved polynucleotide fragments, each having an overhang defined by the first nick and the second nick, wherein the overhang is predesigned by selecting the first and second recognition sites, and (d) assembling the cleaved polynucleotide fragments at the overhangs to form the target nucleic acid, wherein a first overhang of a first cleaved polynucleotide fragment is complementary to a second overhang of a second cleaved polynucleotide fragment, and wherein said assembling comprises ligating the assembled cleaved polynucleotide fragments to produce a ligated product,
A porous current collector for a solid oxide fuel cell including a solid electrolyte layer, a first electrode layer disposed on a side of the solid electrolyte layer, and a second electrode layer disposed on another side of the solid electrolyte layer, the porous current collector comprising: continuous pores and a Ni-Sn alloy layer covering at least a surface of the porous current collector, wherein a Sn content in the Ni-Sn alloy layer is 5% to 30% by weight. continuous pores and a Ni-Sn alloy layer covering at least a surface of the porous current collector,
A sensor controller for use in a position detector for detecting a position of a position indicator on a touch surface, comprising: a controller which is configured to output a value of a symbol to be sent to the position indicator; and a transmitter coupled to the controller, characterised in that the transmitter is configured to generate a transmission signal including a first chip string produced by cyclically shifting a spread code having autocorrelation characteristics by a shift quantity based on the value of the symbol to be sent, and to send the generated transmission signal to the position indicator via the touch surface, wherein the symbol takes multiple values, and the transmitter is configured to generate the transmission signal representing any one of the multiple values based on the shift quantity and non-inversion and inversion of the spread code. a controller which is configured to output a value of a symbol to be sent to the position indicator; and a transmitter coupled to the controller, characterised in that the transmitter is configured to generate a transmission signal including a first chip string produced by cyclically shifting a spread code having autocorrelation characteristics by a shift quantity based on the value of the symbol to be sent, and to send the generated transmission signal to the position indicator via the touch surface, wherein the symbol takes multiple values, and the transmitter is configured to generate the transmission signal representing any one of the multiple values based on the shift quantity and non-inversion and inversion of the spread code.
Spacer to connect a fluid line to a housing section of a flow machine, having a core housing in which a fluid channel is formed to produce a fluid connection between the fluid line and a housing inlet, and having flanges (30, 32, 34, 36) which extend from the core housing and which each have ducts (50, 52) to conduct a fastening means, characterised in that at least two flanges (30, 32) form a lower flange pair to fasten the spacer to the housing section and at least two flanges (34, 36) form an upper flange pair to connect a connection element of the fluid line to the spacer, wherein the flange pairs (10, 12) are arranged to be offset with respect to each other around a channel axis and without a gap with respect to each other in the direction of the channel axis, wherein the ducts (50, 52) of the upper flange pair are opened on the peripheral side.
A memory interface circuit (380, 390) comprising: a first transmit and receive data interface, that is configured to communicate with a second transmit and receive data interface of a second memory interface circuit over a transmission channel, wherein the first transmit and receive data interface includes a selectable on-die termination resistance configured to be turned on when receiving data in a first data transmission mode and turned off when receiving data in a second data transmission mode on a single-ended transmission line of the transmission channel; and a power supply (304a, 304b) electrically coupled to the first transmit and receive data interface and configured to apply a first voltage level for the first data transmission mode and to apply a second voltage level for the second data transmission mode, where the second voltage level is lower than the first voltage level; wherein a voltage swing of the single-ended transmission line at the first transmit and receive data interface is the same in both the first data transmission mode and in the second data transmission mode. a first transmit and receive data interface, that is configured to communicate with a second transmit and receive data interface of a second memory interface circuit over a transmission channel, wherein the first transmit and receive data interface includes a selectable on-die termination resistance configured to be turned on when receiving data in a first data transmission mode and turned off when receiving data in a second data transmission mode on a single-ended transmission line of the transmission channel; and a power supply (304a, 304b) electrically coupled to the first transmit and receive data interface and configured to apply a first voltage level for the first data transmission mode and to apply a second voltage level for the second data transmission mode, where the second voltage level is lower than the first voltage level; wherein a voltage swing of the single-ended transmission line at the first transmit and receive data interface is the same in both the first data transmission mode and in the second data transmission mode.
A compound having the structure of Formula I: or a pharmaceutically acceptable salt thereof, wherein: X is W is CR 4; Y is CR 5; V is CR 6; is selected from a group consisting of the following structures: R 1 is selected from the group consisting of -CO 2 H, tetrazol-5-yl, -NHSO 2 R 11, and -CONHSO 2 R 12; R 2 is selected from the group consisting of -H, -OH, -Cl, -F, and -OCH 3; R 3 is -H; R 4 is selected from the group consisting of -H and -F; R 5 is selected from the group consisting of -H and -F; R 6 is selected from the group consisting of -H, -F, and -Cl; R 7 and R 8 are independently selected from the group consisting of the following structures: R 10 is selected from the group consisting of the following structures: R 11 is -CH 3; R 12 is selected from the group consisting of -CH 3, -CF 3, and -cC 3 H 5. X is W is CR 4; Y is CR 5; V is CR 6; is selected from a group consisting of the following structures: R 1 is selected from the group consisting of -CO 2 H, tetrazol-5-yl, -NHSO 2 R 11, and -CONHSO 2 R 12; R 2 is selected from the group consisting of -H, -OH, -Cl, -F, and -OCH 3; R 3 is -H; R 4 is selected from the group consisting of -H and -F; R 5 is selected from the group consisting of -H and -F; R 6 is selected from the group consisting of -H, -F, and -Cl; R 7 and R 8 are independently selected from the group consisting of the following structures: R 10 is selected from the group consisting of the following structures: R 11 is -CH 3; R 12 is selected from the group consisting of -CH 3, -CF 3, and -cC 3 H 5.
A method of providing an assured service to a user via a virtual private connection in a distributed network, the method comprising: providing an agent in the network; configuring the agent to monitor the virtual private connection by simulating communication over the connection, comprising configuring the agent to simulate activity of other network components and a network component incorporating the agent, wherein the agent is operable to test the network component by simulating the activity of other network components so as to detect faults in the network component or in the provisioned virtual private connection, and wherein said simulating the activity of other network components including simulating a process whereby another network connects or sends data to the network component incorporating the agent; configuring the agent to pass a fault report to a remote network monitoring component in the event that a fault is detected in the provisioned virtual private connection; wherein the agent is arranged to reside on a node of the network other than the user's end node; and wherein a single agent is arranged to receive configuration information identifying a plurality of independent connections to monitor. providing an agent in the network; configuring the agent to monitor the virtual private connection by simulating communication over the connection, comprising configuring the agent to simulate activity of other network components and a network component incorporating the agent, wherein the agent is operable to test the network component by simulating the activity of other network components so as to detect faults in the network component or in the provisioned virtual private connection, and wherein said simulating the activity of other network components including simulating a process whereby another network connects or sends data to the network component incorporating the agent; configuring the agent to pass a fault report to a remote network monitoring component in the event that a fault is detected in the provisioned virtual private connection; wherein the agent is arranged to reside on a node of the network other than the user's end node; and wherein a single agent is arranged to receive configuration information identifying a plurality of independent connections to monitor.
A method for monitoring progression of a cancer or therapeutic efficacy of an anti-cancer treatment in a subject, comprising: a) isolating microvesicles from a first bodily fluid sample that has been collected from a subject having cancer at a first timepoint, and monitoring the status of one or more distinct phosphorylation sites of an oncogenic or tumor-related protein in the microvesicles obtained from the first bodily fluid sample; and b) isolating microvesicles from a second bodily fluid sample that has been collected from the subject having cancer at a second timepoint, the second timepoint occurring after the first timepoint, and monitoring the status of one or more distinct phosphorylation sites of the oncogenic or tumor-related protein in the microvesicles obtained from the second bodily fluid sample; wherein a change in the status of said one or more distinct phosphorylation sites of the oncogenic or tumor-related protein in the microvesicles obtained from the second bodily fluid sample compared to the microvesicles obtained from the first bodily fluid sample indicates progression or regression of the cancer, or indicates therapeutic efficacy or ineffectiveness of the anti-cancer treatment when the first timepoint occurs before the subject has received an anti-cancer treatment, and the second timepoint occurs after the subject has received the anti-cancer treatment; and wherein regression of the cancer or therapeutic efficacy of the anti-cancer treatment is indicated by an increase in the non-active form of the oncogenic or tumor-related protein, and progression of the cancer or ineffectiveness of the anti-cancer treatment is indicated by an increase in the activated form of the oncogenic or tumor-related protein, as determined by the status of said one or more distinct phosphorylation sites detected in the microvesicles, wherein the oncogenic or tumor-related protein is EGFR and the one or more distinct phosphorylation sites is/are selected from the group consisting of T693, Y1110, Y1197, T678 and Y1068. a) isolating microvesicles from a first bodily fluid sample that has been collected from a subject having cancer at a first timepoint, and monitoring the status of one or more distinct phosphorylation sites of an oncogenic or tumor-related protein in the microvesicles obtained from the first bodily fluid sample; and b) isolating microvesicles from a second bodily fluid sample that has been collected from the subject having cancer at a second timepoint, the second timepoint occurring after the first timepoint, and monitoring the status of one or more distinct phosphorylation sites of the oncogenic or tumor-related protein in the microvesicles obtained from the second bodily fluid sample;
A method for estimation of background noise in an audio signal, the method comprising: - obtaining at least one parameter associated with an input audio signal segment based on: - a first linear prediction gain calculated as a quotient between an energy of the input signal and a residual signal energy from a first linear prediction for the audio signal segment; and - a second linear prediction gain calculated as a quotient between the residual signal energy from the first linear prediction and a residual signal energy from a second linear prediction for the audio signal segment; - determining whether the audio signal segment comprises a pause free of speech and music, based at least on the at least one parameter; and: if the audio signal segment is determined to comprise a pause: - updating a background noise estimate based on the audio signal segment. - obtaining at least one parameter associated with an input audio signal segment based on: - a first linear prediction gain calculated as a quotient between an energy of the input signal and a residual signal energy from a first linear prediction for the audio signal segment; and - a second linear prediction gain calculated as a quotient between the residual signal energy from the first linear prediction and a residual signal energy from a second linear prediction for the audio signal segment; - a first linear prediction gain calculated as a quotient between an energy of the input signal and a residual signal energy from a first linear prediction for the audio signal segment; and - a second linear prediction gain calculated as a quotient between the residual signal energy from the first linear prediction and a residual signal energy from a second linear prediction for the audio signal segment; - determining whether the audio signal segment comprises a pause free of speech and music, based at least on the at least one parameter; and: if the audio signal segment is determined to comprise a pause: - updating a background noise estimate based on the audio signal segment. - updating a background noise estimate based on the audio signal segment.
A sealing material obtained by vulcanizing and expanding an intimate mixture comprising a rubber component containing a copolymeric rubber of ethylene, an α-olefin having 3 or more carbon atoms and a non-conjugated diene, a vulcanizing agent, a foaming agent and a thermoplastic resin, characterized in that the intimate mixture includes from 2 to 4 parts by weight of stearic acid and from 5 to 7 parts by weight of calcium oxide per 100 parts by weight of the rubber component, and the sealing material has a stress under 50% compression of 100 kPa or less and wherein the ratio of the parts by weight of stearic acid to the parts by weight of calcium oxide is from 0.5 to 0.75.
An organic electroluminescent element comprising a substrate having thereon an electrode and at least one organic layer, wherein at least one of the organic layers is a light-emitting layer containing a phosphorescent compound and a host compound, and the phosphorescent compound has a HOMO of from -5.15 to -3.50 eV and a LUMO of from -1.25 to +1.00 eV, and the host compound has a 0-0 band of 460 nm or less in a phosphorescence spectrum and the host compound has a glass transition temperature of 60 °C or more, and the host compound is represented by Formula, wherein R 1, R 2 and Ra each represent a hydrogen atom or a substituent; n1, n2 and na each is an integer of 0-4; and X represents O.
Optical device (1, 1', 1", 1′′′) usable in a head-mounted display, the optical device comprising: - a light conducting element having a first refractive index which has an input zone adapted to be coupled to a light source and two first and second opposite main faces (4a and 4b) and which is configured to input and conduct by total internal reflections between said main faces a light received from said light source and to partially output the conducted light out of said light conducting element, - at least one wafer (2, 3) which comprises an internal surface (2a, 3a) facing a said first main face of said light conducting element, and - interposition means (10a, 10b, 10b', 10b") which are in contact with said first main face and said internal surface and which define therebetween a gap (11a, 11b) having a gap refractive index lower than said first refractive index and optically isolating said light conducting element, characterized in that said interposition means comprise a peripheral sealing arrangement (10a, 10b, 10b', 10b") which has a peripheral length and extends between and in sealing contact with a peripheral area (2aa, 3aa) of said internal surface and a zone (4aa, 4ba) of said first main face facing said peripheral area, at least one portion (4") of said sealing arrangement along said peripheral length being configured to exhibit a locally reduced optical coupling with said first main face compared to the remaining of said peripheral length, to preserve said internal reflections at said at least one portion inside said light conducting element. - a light conducting element having a first refractive index which has an input zone adapted to be coupled to a light source and two first and second opposite main faces (4a and 4b) and which is configured to input and conduct by total internal reflections between said main faces a light received from said light source and to partially output the conducted light out of said light conducting element, - at least one wafer (2, 3) which comprises an internal surface (2a, 3a) facing a said first main face of said light conducting element, and - interposition means (10a, 10b, 10b', 10b") which are in contact with said first main face and said internal surface and which define therebetween a gap (11a, 11b) having a gap refractive index lower than said first refractive index and optically isolating said light conducting element,
A pressing apparatus for pressing collected material such as waste, valuable substances and the like for collection in a collection container, having a pressing space which can be filled with collected material from a filling opening and which has a press outlet opening connectable to the collection container; having a ram which is arranged in the pressing space in a manner movable toward and away from the press outlet opening in a linear fashion over the base which is straight in the ram stroke direction; and having a drive apparatus for driving the ram, wherein a front ram side of the ram facing the press outlet opening is arched concavely; wherein the press outlet opening is provided in the oppositely disposed side of the pressing space facing the concavely arched front ram side, the collection container is arranged in the pressing stroke direction of the ram behind the named press outlet opening and the collected material can be pressed by the ram through the named press outlet opening into the collection container by a pressing stroke onto the collection container, characterized in that the concave arching of the front ram side is configured such that an upper marginal section of the front ram side projects in the pressing direction with respect to a central section of the front ram side.
Timepiece comprising a movement including a resonator of the type with a balance /balance spring and an escapement system of the type with a wheel /Swiss lever, the balance being arranged to oscillate at 72,000 vibrations per hour, wherein the wheel of the escapement system has twenty teeth and is at least partially pierced in order to reduce the inertia of the escapement system.
A method of wireless communications at a user equipment, UE, comprising: generating a signaling radio bearer, SRB, packet comprising signaling information, wherein the SRB packet is configured to be transmitted on a channel according to a received scheduling serving grant; determining whether the SRB packet is unable to be transmitted based on resources allocated by the received scheduling serving grant; overriding the received scheduling serving grant in response to determining that the SRB packet is unable to be transmitted; and transmitting at least a part of the SRB packet on the channel. generating a signaling radio bearer, SRB, packet comprising signaling information, wherein the SRB packet is configured to be transmitted on a channel according to a received scheduling serving grant; determining whether the SRB packet is unable to be transmitted based on resources allocated by the received scheduling serving grant; overriding the received scheduling serving grant in response to determining that the SRB packet is unable to be transmitted; and transmitting at least a part of the SRB packet on the channel.