A method for determining zygosity of a canola plant comprising a fad-2 gene, said method comprising: obtaining a genomic DNA sample from said canola plant; producing a contacted sample by contacting said genomic DNA sample with a first primer and a second primer, wherein said first primer consists of SEQ ID NO:2 binding a region of said fad- 2 gene upstream of a location of a single nucleotide polymorphism of interest, said second primer consists of SEQ ID NO:3 binding a region of said fad-2 gene downstream of the single nucleotide polymorphism of interest, wherein said first primer and said second primer produce an amplicon when subjected to polymerase chain reaction conditions; subjecting said contacted sample to PCR conditions, wherein said amplicon is produced; allowing each of a first fluorescent probe, the sequence of which consists of SEQ ID NO:5, and a second fluorescent probe, the sequence of which consists of SEQ ID NO:4, to hybridize to the amplicon for a period of time and at a temperature between 50 - 70 degrees Celsius, said first fluorescent probe preferentially hybridizing to said amplicon when said single nucleotide polymorphism of interest is not present in said amplicon, said second fluorescent probe preferentially hybridizing to said amplicon when said single nucleotide polymorphism of interest is present in said amplicon; increasing said temperature after the period of time specified in the allowing step; capturing said fluorescence produced by each of said first and second probes during the step of increasing; and determining zygosity of said canola plant, the step of determining comprising a comparison of the fluorescence produced by each of the first and second probes, wherein the fluorescence of the first and second probes predominantly reflecting the fluorescence produced in a homozygous SNP positive control sample indicating the presence of said single nucleotide polymorphism of interest, the fluorescence of the first and second probes predominantly reflecting the fluorescence produced in a homozygous SNP negative control sample indicating a lack of the presence of said single nucleotide polymorphism of interest, and the fluorescence of the first and second probes predominantly reflecting the fluorescence produced in a heterozygous SNP positive control sample indicating said canola plant comprises a first allele including said single nucleotide polymorphism of interest and a second allele lacking said single nucleotide polymorphism of interest. obtaining a genomic DNA sample from said canola plant; producing a contacted sample by contacting said genomic DNA sample with a first primer and a second primer, wherein said first primer consists of SEQ ID NO:2 binding a region of said fad- 2 gene upstream of a location of a single nucleotide polymorphism of interest, said second primer consists of SEQ ID NO:3 binding a region of said fad-2 gene downstream of the single nucleotide polymorphism of interest, wherein said first primer and said second primer produce an amplicon when subjected to polymerase chain reaction conditions; subjecting said contacted sample to PCR conditions, wherein said amplicon is produced; allowing each of a first fluorescent probe, the sequence of which consists of SEQ ID NO:5, and a second fluorescent probe, the sequence of which consists of SEQ ID NO:4, to hybridize to the amplicon for a period of time and at a temperature between 50 - 70 degrees Celsius, said first fluorescent probe preferentially hybridizing to said amplicon when said single nucleotide polymorphism of interest is not present in said amplicon, said second fluorescent probe preferentially hybridizing to said amplicon when said single nucleotide polymorphism of interest is present in said amplicon; increasing said temperature after the period of time specified in the allowing step; capturing said fluorescence produced by each of said first and second probes during the step of increasing; and determining zygosity of said canola plant, the step of determining comprising a comparison of the fluorescence produced by each of the first and second probes, wherein the fluorescence of the first and second probes predominantly reflecting the fluorescence produced in a homozygous SNP positive control sample indicating the presence of said single nucleotide polymorphism of interest, the fluorescence of the first and second probes predominantly reflecting the fluorescence produced in a homozygous SNP negative control sample indicating a lack of the presence of said single nucleotide polymorphism of interest, and the fluorescence of the first and second probes predominantly reflecting the fluorescence produced in a heterozygous SNP positive control sample indicating said canola plant comprises a first allele including said single nucleotide polymorphism of interest and a second allele lacking said single nucleotide polymorphism of interest.