Microfluidic cell culture chip that contains a central module comprising: - a central unit, which contains -- a support consisting of a non-absorbable membrane, including an upper face and a lower face, perforated by at least one perforation, said support being composed in particular of bioprinted plastic, polycarbonate, tissue culture plastic, glass or SU8, -- a 3D nanostructured porous membrane, including an upper face and a lower face, and including at least one protrusion, said at least one protrusion being hollow and including an outer face and an inner face and forming a structure in relief on the upper face side of the 3D nanostructured membrane, said protrusion being in particular in the shape of a hollow dome having a circular base, oo said lower face of said 3D nanostructured porous membrane being positioned in a rigidly connected manner on said upper face of said support, oo or said upper face of the 3D nanostructured membrane being positioned in a rigidly connected manner on said lower face of said support, oo and said 3D nanostructured porous membrane and the at least one protrusion being composed of materials suitable for the culture of two types of distinct cells; - a base, said central unit being integrated into said base, and forming a whole with said base. - a central unit, which contains -- a support consisting of a non-absorbable membrane, including an upper face and a lower face, perforated by at least one perforation, said support being composed in particular of bioprinted plastic, polycarbonate, tissue culture plastic, glass or SU8, -- a 3D nanostructured porous membrane, including an upper face and a lower face, and including at least one protrusion, said at least one protrusion being hollow and including an outer face and an inner face and forming a structure in relief on the upper face side of the 3D nanostructured membrane, said protrusion being in particular in the shape of a hollow dome having a circular base, oo said lower face of said 3D nanostructured porous membrane being positioned in a rigidly connected manner on said upper face of said support, oo or said upper face of the 3D nanostructured membrane being positioned in a rigidly connected manner on said lower face of said support, oo and said 3D nanostructured porous membrane and the at least one protrusion being composed of materials suitable for the culture of two types of distinct cells; -- a support consisting of a non-absorbable membrane, including an upper face and a lower face, perforated by at least one perforation, said support being composed in particular of bioprinted plastic, polycarbonate, tissue culture plastic, glass or SU8, -- a 3D nanostructured porous membrane, including an upper face and a lower face, and including at least one protrusion, said at least one protrusion being hollow and including an outer face and an inner face and forming a structure in relief on the upper face side of the 3D nanostructured membrane, said protrusion being in particular in the shape of a hollow dome having a circular base, oo said lower face of said 3D nanostructured porous membrane being positioned in a rigidly connected manner on said upper face of said support, oo or said upper face of the 3D nanostructured membrane being positioned in a rigidly connected manner on said lower face of said support, oo and said 3D nanostructured porous membrane and the at least one protrusion being composed of materials suitable for the culture of two types of distinct cells; - a base, said central unit being integrated into said base, and forming a whole with said base.