A method for collecting one or more immune cells in dependence on their binding force with a sample surface that has been prepared with target cells, the method comprising: - providing a sample holder comprising a holding space, wherein the holding space comprises a fluid medium and comprises the sample surface with target cells and the one or more immune cells in the fluid medium, wherein the one or more immune cells are contacting said sample surface with target cells and wherein the one or more immune cells comprise a first set of one or more immune cells and a second set of one or more immune cells, and - rotating the sample holder around an axis of rotation for causing a centrifugal force on the one or more immune cells herewith separating the one or more immune cells from the sample surface with target cells, said rotating comprising: - rotating the sample holder at a first angular velocity, ω 1, the sample surface with target cells being positioned a distance, r 1, from the axis of rotation, ω 1 2 r 1 having a first value, for causing a centrifugal force on the one or more immune cells herewith separating the first set of one or more immune cells from the sample surface with target cells, and - optionally, collecting the separated first set of one or more immune cells in the fluid medium after they have been separated from the sample surface with target cells, and - rotating the sample holder at a second angular velocity, ω 2, the sample surface with target cells being positioned a distance, r 2, from the axis of rotation, ω 2 2 r 2 having a second value higher than said first value, for causing a centrifugal force on one or more immune cells still contacting the sample surface with target cells herewith separating the second set of one or more immune cells from the sample surface with target cells, and - collecting, separately from the first set of one or more immune cells, the separated second set of one or more immune cells in the fluid medium after they have been separated from the sample surface with target cells. - providing a sample holder comprising a holding space, wherein the holding space comprises a fluid medium and comprises the sample surface with target cells and the one or more immune cells in the fluid medium, wherein the one or more immune cells are contacting said sample surface with target cells and wherein the one or more immune cells comprise a first set of one or more immune cells and a second set of one or more immune cells, and - rotating the sample holder around an axis of rotation for causing a centrifugal force on the one or more immune cells herewith separating the one or more immune cells from the sample surface with target cells, said rotating comprising: - rotating the sample holder at a first angular velocity, ω 1, the sample surface with target cells being positioned a distance, r 1, from the axis of rotation, ω 1 2 r 1 having a first value, for causing a centrifugal force on the one or more immune cells herewith separating the first set of one or more immune cells from the sample surface with target cells, and - optionally, collecting the separated first set of one or more immune cells in the fluid medium after they have been separated from the sample surface with target cells, and - rotating the sample holder at a second angular velocity, ω 2, the sample surface with target cells being positioned a distance, r 2, from the axis of rotation, ω 2 2 r 2 having a second value higher than said first value, for causing a centrifugal force on one or more immune cells still contacting the sample surface with target cells herewith separating the second set of one or more immune cells from the sample surface with target cells, and - collecting, separately from the first set of one or more immune cells, the separated second set of one or more immune cells in the fluid medium after they have been separated from the sample surface with target cells. - rotating the sample holder at a first angular velocity, ω 1, the sample surface with target cells being positioned a distance, r 1, from the axis of rotation, ω 1 2 r 1 having a first value, for causing a centrifugal force on the one or more immune cells herewith separating the first set of one or more immune cells from the sample surface with target cells, and - optionally, collecting the separated first set of one or more immune cells in the fluid medium after they have been separated from the sample surface with target cells, and - rotating the sample holder at a second angular velocity, ω 2, the sample surface with target cells being positioned a distance, r 2, from the axis of rotation, ω 2 2 r 2 having a second value higher than said first value, for causing a centrifugal force on one or more immune cells still contacting the sample surface with target cells herewith separating the second set of one or more immune cells from the sample surface with target cells, and - collecting, separately from the first set of one or more immune cells, the separated second set of one or more immune cells in the fluid medium after they have been separated from the sample surface with target cells.