A time-division segment block-based handwritten signature authentication system, comprising: a handwritten signature input unit that includes a touch input unit configured to output touch data, as a handwritten signature input data, including position data and pressure data for positions that are touched by a signer for handwritten signature; an enrollment unit configured to enroll handwritten signature characteristics information of each signer; and a handwritten signature authentication unit configured to count time when the handwritten signature input data is entered, store the handwritten signature input data with timestamp information, recognize segments divided by a predetermined time slice (t 1) from the handwritten signature input data and the timestamp information, generate a handwritten signature image and segment images by identifying a handwritten signature, generate a handwritten signature block containing the handwritten signature image and segment blocks containing the segment images, collect handwritten signature characteristics information (Σ') including handwritten signature block information, each segment block information, correlation information between the segment blocks, and correlation information between each segment block and the handwritten signature block, map collected handwritten signature characteristics information to identification information of the signer, enroll the collected handwritten signature characteristics information in the enrollment unit, collect handwritten signature characteristics information (Σ) including handwritten signature block information, each segment block information, correlation information between the segment blocks, and correlation information between each segment block and the handwritten signature block from the touch data entered through the touch input unit of the handwritten signature input unit upon request for handwritten signature authentication, load enrolled handwritten signature characteristics information (Σ') that corresponds with the identification information of the signer who requests handwritten signature authentication, and perform a segment-block-based handwritten signature authentication according to a match rate by comparing the enrolled handwritten signature characteristics information (Σ') with the collected handwritten signature characteristics information (Σ). a handwritten signature input unit that includes a touch input unit configured to output touch data, as a handwritten signature input data, including position data and pressure data for positions that are touched by a signer for handwritten signature; an enrollment unit configured to enroll handwritten signature characteristics information of each signer; and a handwritten signature authentication unit configured to count time when the handwritten signature input data is entered, store the handwritten signature input data with timestamp information, recognize segments divided by a predetermined time slice (t 1) from the handwritten signature input data and the timestamp information, generate a handwritten signature image and segment images by identifying a handwritten signature, generate a handwritten signature block containing the handwritten signature image and segment blocks containing the segment images, collect handwritten signature characteristics information (Σ') including handwritten signature block information, each segment block information, correlation information between the segment blocks, and correlation information between each segment block and the handwritten signature block, map collected handwritten signature characteristics information to identification information of the signer, enroll the collected handwritten signature characteristics information in the enrollment unit, collect handwritten signature characteristics information (Σ) including handwritten signature block information, each segment block information, correlation information between the segment blocks, and correlation information between each segment block and the handwritten signature block from the touch data entered through the touch input unit of the handwritten signature input unit upon request for handwritten signature authentication, load enrolled handwritten signature characteristics information (Σ') that corresponds with the identification information of the signer who requests handwritten signature authentication, and perform a segment-block-based handwritten signature authentication according to a match rate by comparing the enrolled handwritten signature characteristics information (Σ') with the collected handwritten signature characteristics information (Σ).