Preparations of Albumin and Sulphonized Carbon Compounds, and Process of Making the same.
Year: 1899

page_text: Date of Application, 12th July, 1899
Complete Specification Left, 11th Apr., 1900-Accepted, 12th May, 1900
Preparations of Albumin and Sulphonized Carbon Compounds, and
Process of Making the same.
I, Dr. LUDWIG OTTO HELMERS, of 174, Fuhlsbüttelerstrasse, in the City of
Hamburg and Empire of Germany, Chemist, do hereby declare the nature of
this invention to be as follows:-
Albumin solutions are not precipitated by the salts of ichthyol sulphonic acid
from a sufficiently diluted solution, even when the said solution is heated to
boiling point.
Like the ichthyol salts, in the presence of albumin also behave other substances
which are obtained by the action of sulphuric acid on mineral-oils and similar
hydro-carbons containing sulphur, viz. the so-called petro-sulfol, thiol and
the like.
According to the British Patent No. 11344 A.D. 1897 precipitations from
these solutions take place only in the presence of a stronger acid, or by employing
free sulphonic acids, respectively. Experiments however have shown that such
precipitations containing albumin can also be obtained by an addition of suffi-
cient quantities of salt to the watery solutions of albumin and the aforesaid
substance.
The precipitations obtained at ordinary temperature by the alkali salts, are, in
contrast to the substances obtained by the aforesaid British Patent, soluble in
pure water. They become insoluble by drying and heating for a somewhat
longer time with a salt solution in excess. These products, insoluble in water,
can be obtained directly by producing the precipitation at a higher temperature.
Some salts, such as sodium phosphate precipitate solutions of albumin and the
said sulphur containing substances, only by heating.
When treating such albumin solutions with salts of the earth alkalis or with
salts of metals it will not be necessary to heat the solution since precipitations,
insoluble in water, already take place at ordinary temperature so that they need
only be washed out and dried.
The substances thus obtained in one or the other way by precipitation with
salts contain besides the sulphonic acids and albumin, also the base of the salt
utilized and therein are different from those described in the aforesaid British
Patent. These new chemical compounds are precipitated in the form of slimy
products containing water which, after drying at about 100° C., are easily pul-
verizable and are of a brown colour, without smell or taste, insoluble in water and
nearly insoluble in the usual organic solvents. By heating them with acids
they split off the base and thus become converted into the products of the afore-
said British Patent No. 11344 A.D. 1897; when being heated with alkali they
swell and gradually become completely dissolved.
Dated this 12th day of July 1899
HASELTINE, LAKE & Co.,
45, Southampton Buildings, London, W.C.,
Agents for the Applicant.

page_text: Preparations of Albumin and Sulphonized Carbon Compounds, and
Process of Making the same.
I, Dr. LUDWIG OTTO HELMERS, of 174, Fuhlsbüttelerstrasse, in the City of
Hamburg, and Empire of Germany, Chemist, do hereby declare the nature of
this invention and in what manner the same is to be performed, to be particularly
described and ascertained in and by the following statement:-
―
Albumin solutions are not precipitated by the alkali salts of ichthyol sulphonic
acid from a sufficiently diluted solution, even when the said solution is heated
to boiling point.
Other substances which are obtained by the action of sulphuric acid on
mineral-oils and similar hydrocarbons containing sulphur, viz. the so-called
petrosulphol, thiol and the like, also behave like the ichthyol salts in the pre-
sence of albumin.
According to the British Patent No. 11344 A.D. 1897 precipitations from
these solutions take place only in the presence of a stronger acid, or by employing
free sulphonic acids, respectively. Experiments however have shown that such
precipitations containing albumin can also be obtained by an addition of suffi-
cient quantities of salt to the watery solutions of albumin and the aforesaid
substances.
The precipitations obtained at ordinary temperature by the alkali salts, are, in
contrast to the substances obtained by the aforesaid British Patent, soluble in
pure water. They become insoluble by drying and heating for a somewhat
longer time with a salt solution in excess. These products, insoluble in water,
can be obtained directly by producing the precipitation at a higher temperature.
Some salts, such as sodium phosphate precipitate solutions of albumin and the
said sulphur containing substances, only by heating.
When treating such solutions of albumin and the alkali salts of ichthyol,
petrosulphol and the like, with salts of the earth alkalies or with salts of metals,
or when mixing albumin solutions with soluble earth-alkaline or metal salts of
the above substances, it will not be necessary to heat the solution, since precipi-
tations, insoluble in water, already take place at ordinary temperature, so that
they need only be washed out and dried.
The substances thus obtained in one or the other way by precipitation with
salts, contain besides the sulphonic acids and albumin, also the base of the salt
utilized and therein are different from those described in the aforesaid British
Patent. When the precipitation is produced at ordinary temperature and
effected by means of salts which do not coagulate albumin, the residues or pre-
cipitates contain the albumin always in a constant proportion, as the albumin
added in excess remains in solution. The albumin compounds are precipitated
in the form of slimy products containing water, which, after drying at about
100° C., are easily pulverizable and are of a brown colour, without smell or
taste, insoluble in water and nearly insoluble in the usual organic solvents. By
heating them with acids they split off the base and thus become converted into
the products of the aforesaid British Patent No. 11344 A.1). 1897; when being
heated with alkali they swell and gradually become completely dissolved.
The following is given as an example of the process:
a) 1 kg. petrosulphol is dissolved in 3 litres of water and then added to a
solution of 1 kg. of albumin in 10 litres of water. This mixture is precipitated
by gradually stirring it with 20 kgs. of a 10% ammonium chloride solution.
After the precipitation is completed, the liquor containing albumin and ammonium

page_text: chloride is poured off and to the precipitated residue is added a solution of
ammonium chloride which is then heated for several hours and afterwards the
insoluble precipitation is pressed and washed in hot water and finally dried at
about 1000 and pulverized.
b) The solutions of 1 kg. of ichthyol ammonium in 15 litres of water and
1 kg. of albumin in 10 litres of water are precipitated with 3 kgs. of a 10%
calcium chloride solution. The precipitation is freed of its liquor by pressing
and afterwards washed with water and dried.
Having now particularly described and ascertained the nature of my said
invention and in what manner the same is to be performed, I declare that what
I claim is
1. The process which consists in treating an aqueous solution of a mixture of -
albumin and a product derived from a sulphureted hydrocarbon combined with
sulphuric acid, with a solution of a suitable salt, whereby an insoluble compound
is formed and precipitated, substantially as set forth.
2. A compound in the form of a brown powder, consisting of albumin, a base,
and a product derived from a sulphureted hydrocarbon combined with sulphuric
acid, said compound being insoluble in water and other solvents and tasteless ag
well as odourless, substantially as set forth.
D
111 .
0
1 1000
HASELTINE, LAKE & Co.,
45, Southampton Buildings, London, W.C.,
Agents for the Applicant.