Process for Obtaining an Iron Albumen Derivative from Animal Organs.
Year: 1893

page_text: Date of Application, 4th Sept., 1893
Complete Specification Left, 16th Mar., 1894-Accepted, 5th May, 1894
PROVISIONAL SPECIFICATION.
Process for Obtaining an Iron Albumen Derivative from
Animal Organs.
I, BENJAMIN WILLCOX of 47 Lincoln's Inn Fields in the County of Middlesex
Patent Agent do hereby declare the nature of this invention which has been
communicated to me from abroad by C. F. Boehringer & Soehne of Waldhof
near Mannheim in the Empire of Germany to be as follows:-
:-
Certain internal animal organs such as the liver, the spleen and the kidneys
contain an iron albumen derivative in which the iron is bound in such a manner
that it cannot be directly identified in a solution of the preparation by the aid of
the reagents usually employed for iron.
This iron albumen derivative is present in large quantities in the liver and in
order to obtain or extract the same the operation is conducted as hereinafter
described.
The liver being cut in small pieces or minced is stirred without the addition
This mixture is
of other matter into five times its volume of distilled water.
heated on a water bath and stirred for about two hours the temperature being
gradually raised to about 95 degrees centigrade that is to say until the albumenous
substances commence to coagulate. The gradual heating is for the purpose of
completely extracting the liver paste before the coagulation commences.
When the said coagulation has commenced being indicated by the appearance
of a thick bloody liquid between the solid parts of the mass the temperature is
raised to boiling and maintained at this point during from about five to eight
minutes until the last trace of blood has coagulated and the liquid has become
clear. The boiling must not be continued longer than is absolutely necessary
in order that no formation of glue may take place. The materials are then
filtered and washed with boiling water. The brownish yellow liquid obtained
having a slightly acid reaction is allowed to become cold and a diluted solution
of tartaric acid or other suitable acid is carefully introduced so long as the
deposit formed is materially coloured. The brown yellow deposit or precipitate
formed soon becomes flocculent and falls to the bottom of the vessel. The
a filter and
thick muddy liquid is poured off the deposit being collected on
washed with distilled water until the acid reaction has disappeared.
The washed deposit is dried at a moderately high temperature preferably in a
vacuum and pulverized.
The iron albumen derivative thus obtained is in the form of an odourless and
tasteless powder of reddish brown colour which is insoluble in water alcohol
and diluted acids. In weak alkaline liquids it dissolves readily in a brown red
colour. A neutral solution does not coagulate when boiling. It contains from
six to seven per cent. of iron which is bound in another form than that of a salt.
When 20 cubic centimetres of the solution obtained by boiling 0.06 grammes
of the iron albumen derivative with a small quantity of ammonia are mixed while
cold with one drop of a solution of hydro sulphuret of ammonia containing
50 per cent. of sulphuret (Tresenius Qualitative Analysis 14th edition page 68) the
solution does not lose its yellow colour within three minutes.

page_text: By treating other organs such as the spleen or kidneys in an analogous manner a
similar product is obtained.
Dated this 4th day of September 1893.
J. H. JOHNSON & Co.,
47, Lincoln's Inn Fields, Agents.
COMPLETE SPECIFICATION.
Process for Obtaining an Iron Albumen Derivative from
Animal Organs.
I, BENJAMIN WILLCOX of 47 Lincoln's Inn Fields in the County of Middlesex
Patent Agent do hereby declare the nature of this invention (which has been
communicated to me from abroad by C. F. Boehringer & Soehne of Waldhof
near Mannheim in the Empire of Germany) and in what manner the same is to
be performed to be particularly described and ascertained in and by the following
statement:-
Certain internal animal organs such as the liver, the spleen, and the kidneys,
contain an iron albumen derivative in which the iron is bound in such a manner
that it cannot be directly identified in a solution of the preparation by the aid of
the reagents usually employed for iron. This iron albumen derivative is present
in large quantities in the liver, and, in order to obtain, or extract the same, the
operation is conducted as hereinafter described.
The liver being cut in small pieces or minced is stirred without the addition of
other matter into five times its volume of distilled water. This mixture is heated
on a water bath and stirred for about two hours the temperature being gradually
raised to about 95 degrees centigrade that is to say until the albumenous substances
commence to coagulate. The gradual heating is for the purpose of completely
extracting the liver paste before the coagulation commences.
When the said coagulation has commenced to be indicated by the appearance of
a thick bloody liquid between the solid parts of the mass the temperature is raised
to boiling and maintained at this point during from about five to eight minutes
until the last trace of blood has coagulated and the liquid has become clear. The
boiling must not be continued longer than is absolutely necessary in order that no
formation of glue may take place. The materials are then filtered and washed with
boiling water. The brownish yellow liquid having a slightly acid reaction so
obtained is allowed to become cold and a diluted solution of tartaric acid or other
suitable acid is carefully introduced so long as the deposit formed is materially
coloured. The brownish-yellow deposit or precipitate formed soon becomes
flocculent and falls to the bottom of the vessel. The thick muddy liquid is poured
off or otherwise removed the deposit being collected on a filter and washed with
distilled water until the acid reaction has disappeared.
The washed deposit is dried at a moderately high temperature (preferably in a
vacuum) and pulverized.
The iron albumen derivative thus obtained is in the form of an odourless and
tasteless powder of reddish-brown colour which is insoluble in water, alcohol and
diluted acids. In weak alkaline liquids it dissolves readily with a brown-red colour.
A neutral solution does not coagulate when boiling. It contains from six to
seven per cent. of iron which is bound in another form than that of a salt.
When 20 cubic centimetres of the solution obtained by boiling 0.06 grammes of
the iron albumen derivative with a small quantity of ammonia are mixed while
cold with one drop of a solution of hydro sulphuret of ammonia containing
50 per cent. of sulphuret (Tresinius Qualitative Analysis 14th edition page 68) the
solution does not lose its yellow colour within three minutes.
By treating other organs such as the spleen or kidneys in an analogous manner a
similar product is obtained.

page_text: Having now particularly described and ascertained the nature of our inven--
tion and in what manner the same is to be performed we declare that what we
claim is
A process for obtaining an iron albumen derivative from animal organs such as
the liver, spleen and kidneys consisting in gradually heating the reduced or minced
organs with water to boiling point and extracting and mixing the extracted solution
with diluted acid until a brownish yellow precipitate forms substantially as
hereinbefore described.
Dated the 16th day of March 1894.
JOHNSONS & WILLCOX,
47, Lincoln's Inn Fields, London, Agents.