IMPROVED PREPARA- TIONS OF ANIMAL VACCINE
Year: 1882

page_text: LETTERS PATENT to Edward Thomas Darke of Hemming's Row Charing
"
Cross in the County of Middlesex for an Invention of "IMPROVED PREPARA-
TIONS OF ANIMAL VACCINE A communication to him from abroad by
Dr. Evariste Warlomont, Member of the Royal Academy of Belgium residing
at 74 De la Toison d'Or Brussels in the Kingdom of Belgium
PROVISIONAL SPECIFICATION left by the said Edward Thomas Darke at
the Office of the Commissioners of Patents on the 28th September 1882.
EDWARD THOMAS DARKE of Hemmings Row Charing Cross in the County of
Middlesex "IMPROVED PREPARATIONS OF ANIMAL VACCINE [A communication
to him from abroad by Dr. Evariste Warlomont, Member of the Royal Academy
of Belgium residing at 74 De la Toison d'Or Brussels in the Kingdom of
Belgium.]
"
This Invention has for its object improved preparations of animal vaccine.
These preparations may either be in the form of an emulsion or salve.
The process is as follows:-The vaccine (cow-pox or horse-pox) is disseminated
in animals of the bovine race by introducing it into the derma either by points or
by scarifications. When the pustules have come to a head, after a time which does
not exceed six days, the vaccine is extracted from them in the following manner :-
The pustule pinched at its base in the jaws of a pair of forceps is freed from the
impurities with which it is covered by means of a rapid superficial scraping which
removes the scurf, foreign bodies and blood and eventually the last particle of pus.
The portions of skin which may still adhere are next stripped off and then by
means of a sharp knife the pustule so cleaned and decorticated is removed close to
the compressing forceps. The pustules so detached and only containing vaccine
pulp properly so called are next cut up into as small pieces as possible and collected
in a porcelain mortar where they are again cut and chopped up small. This being
done they are diluted with an equal volume of a mixture of equal parts of distilled
water and rectified glycerine triturated a long time and passed through a filter of
metallic cloth.
The liquid which passes through the filter is mixed.

page_text: 1. If it is wished to make the emulsion with an equal quantity of rectified
glycerine.
2. If it is wished to make the salve with an equal quantity of glycerised starch
with which the liquid is intimately mixed by trituration in a porcelain mortar.
The emulsion is put into tubes of deep amber coloured glass which are closed
with a mixture of equal parts of paraffin and tallow and the ends are then plunged
into a solution of india rubber in ether which preserves them from outside
influences and insures complete stoppage of the tubes.
The salve is kept in small phials of amber glass with ground stoppers.
The special character of the invention is the extraction of all the active part of
the vaccinal pustule and the preservation sheltered from the ordinary causes of
alteration of organic matters.
The vaccine so prepared has a very great strength and can be kept a long time.

page_text: SPECIFICATION in pursuance of the conditions of the Letters Patent filed
by the said Edward Thomas Darke in the Great Seal Patent Office on the
27th March 1883.
EDWARD THOMAS DARKE of Hemmings Row Charing Cross in the County of
Middlesex "IMPROVED PREPARATIONS OF ANIMAL VACCINE
" A communication
to me from abroad by Dr Evariste Warlomont, Member of the Royal Academy of
Belgium residing at 74 De la Toison d'Or Brussels in the Kingdom of Belgium
This Invention has for its object improved preparations of animal vaccine.
These preparations may either be in the form of an emulsion or salve.
The process is as follows:-The vaccine (cow-pox or horse-pox) is disseminated
in animals of the bovine race by introducing it into the derma either by points or
by scarifications. When the pustules have come to a head, after a time which does
not exceed six days, the vaccine is extracted from them in the following manner :-
The pustule pinched at its base in the jaws of a pair of forceps is freed from the
impurities with which it is covered by means of a rapid superficial scraping which
removes the scurf foreign bodies and blood and eventually the last particle of pus.
The portions of skin which may still adhere are next stripped off and then by
means of a sharp knife the pustule so cleaned and decorticated is removed close to
the compressing forceps. The pustules so detached and only containing vaccine
pulp properly so called are next cut up into as small pieces as possible and collected
in a porcelain mortar where they are again cut and chopped up small. This being
done they are diluted with an equal volume of a mixture of equal parts of distilled
water and rectified glycerine, triturated a long time and passed through a filter of
metallic cloth.
The liquid which passes through the filter is mixed.
1. If it is wished to make the emulsion with an equal quantity of rectified
glycerine.
2. If it is wished to make the salve, with an equal quantity of glycerised starch
with which the liquid is intimately mixed by trituration in a porcelain mortar.
The emulsion is put into tubes of deep amber coloured glass which are closed with
a mixture of equal parts of paraffin and tallow and the ends are then plunged into
a solution of india rubber in ether which preserves them from outside influences
and insures complete stoppage of the tubes.
The salve is kept in small phials of amber glass with ground stoppers.
The special character of the invention is the extraction of the active part of the
vaccinal pustule and its preparation substantially as described so as to protect and
preserve it from the ordinary causes of alteration of organic matters.
Vaccine, so prepared, has very great strength and can be kept a long time.
What I claim is :-
Preparations of animal vaccine substantially as herein described.
In witness whereof I the said Edward Thomas Darke have hereunto set my
hand and seal this twenty second day of March in the year of our Lord One
thousand eight hundred and eighty three.