Production of a Medical Preparation from the Tubercle Bacillus, and the Process employed therefor.
Year: 1893

page_text: Date of Application, 16th Nov., 1893
Complete Specification Left, 5th July, 1894-Accepted, 11th Aug., 1894
PROVISIONAL SPECIFICATION.
Production of a Medical Preparation from the Tubercle Bacillus,
and the Process employed therefor.
I, Professor Dr. EDWIN KLEBS, of No. 8 Jahn Strasse, Karlsruhe, Baden,
Germany, do hereby declare the nature of this invention to be as follows:-
According to my invention I produce from the product resulting from the change
of matter in the tubercle bacillus a substance which I term " antiphthisin" and
which I have found to act in a healing manner upon persons affected with
tubercles.
I obtain this substance in the following manner.
I do not kill by heat the tubercle bacilli reared in ordinary alimentary fluid
because I have found that, by boiling, the antiphthisin is for the most part
destroyed, but I add to such fluid per cent. of cresol, and after letting this mixture
stand for 24 hours, I then filter it. The fluid filtrate obtained in this manner free
from bacilli is then mixed with a solution of sodium-bismuth-iodide, acidified by
means of acetic acid; so long as a deposit continues to be formed, the said
solution allowing the toxalbumoses to separate from the sozalbumoses, the
antiphthisin.
I then again filter the product and render the filtrate weakly alkaline by means
of soda lye, after which I heat it gently in a water bath and again filter it.
This
By adding to the filtrate five times the quantity of alcohol the substance which
I term antiphthisin is precipitated from the entirely colourless filtrate.
precipitate is washed with alcohol, and is finally brought into a form fit for use by
dissolving it in water containing 0.2 per cent. of ortho cresol.
The albumose in light yellow or madeira coloured solution thus obtained is
applied medicinally by injecting it into the strait-gut and the head of the wind pipe
in quantities varying from one to fifty cube centimetres, according to the contents
of the original culture fluid.
Dated this 16th day of November 1893.
ABEL & IMRAY,
Agents for the Applicant.
COMPLETE SPECIFICATION.
Production of a Medical Preparation from the Tubercle Bacillus,
and the Process employed therefor.
1, Professor Dr. EDWIN KLEBS, of No. 8 Jahn Strasse, Karlsruhe, Baden,
Germany, do hereby declare the nature of this invention and in what manner the
same is to be performed, to be particularly described and ascertained in and by
the following statement :-
.6
According to my invention I produce from the product resulting from the change
of matter in the tubercle bacillus a substance which I term antiphthisin" and
which I have found to act in a healing manner upon persons affected with
tubercles.
I obtain this substance in the following manner.
I do not kill by heat the tubercle bacilli reared in ordinary alimentary fluid
because I have found that, by boiling, the antiphthisin is for the most part
destroyed, but I add to such fluid per cent. of cresol, and after letting this mixture
D

page_text: stand for 24 hours, I then filter it. The fluid filtrate obtained in this manner
free from bacilli is then mixed with a solution of sodium-bismuth-iodide, acidified
by means of acetic acid, so long as a deposit continues to be formed, the said
solution allowing the toxalbumoses to separate from the sozalbumoses, the
antiphthisin.
I then again filter the product and render the filtrate weakly alkaline by
means of soda lye, after which I heat it gently in a water bath and again
filter it.
By adding to the filtrate five times the quantity of alcohol the substance which
I term antiphthisin is precipitated from the entirely colourless filtrate.
This precipitate is washed with alcohol, and is finally brought into a form fit for
use by dissolving it in water containing 0.2 per cent. of ortho cresol.
The albumose in light yellow or madeira coloured solution thus obtained is
applied medicinally by injecting it into the strait gut and the head of the wind pipe
in quantities varying from one to fifty cube centimetres, according to the contents
of the original culture fluid.
Having now particularly described and ascertained the nature of this inven-
tion and in what manner the same is to be performed, I declare that what I
claim is :-
1. The process for producing from tubercle bacillus a substance termed
antiphthisin, consisting in adding to the tubercle culture fluid at ordinary temperature
about one half per cent. of cresol and after separation of the fluid from the bacilli,
by filtration, mixing with the fluid sodium-bismuth-iodide in excess, then
eliminating from the filtrate of the produced deposit the excess of bismuth by
adding soda lye and again filtering and lastly precipitating from the fluid obtained
the said antiphthisin by the addition of alcohol, substantially as described.
2. As a new product the body termed antiphthisin obtained as set forth in the
first claim.
Dated this 5th day of July 1894.
ABEL & IMRAY,
Agents for the Applicant.