An Improved Preparation of Nucleins and Process for Making it.
Year: 1897

page_text: Date of Application, 27th July, 1897--Accepted, 2nd Oct.,
1897
COMPLETE SPECIFICATION.
An Improved Preparation of Nucleins and Process for Making it.
I, JOHN CARNRICK, of 426 and 428 West Broadway, in the City and State of
New York, United States of America, Manufacturing Chemist, do hereby declare
the nature of this invention and in what manner the same is to be performed, to
be particularly described and ascertained in and by the following statement:-
This invention relates to a nutritive and antitoxic preparation, adapted more
particularly for stimulating assimilative and nutritive action in the formation of
bodily tissue and for destroying poisonous germs and ptomaines, which produce
various zymotic diseases.
The object of my invention is to provide a nutritive or tissue-building composi-
tion containing nuclein from different portions of the animal organisi adapted
for restoring the vitality of all the tissues of the human organisms by aiding and
supporting assimilative nutrition and also for destroying toxic germs and prevent-
ing infectious and zymotic diseases, such as Diphtheria, Typhoid Fever, Scarlet
Fever, Cholera Infantum, Tuberculosis, Malaria, and other diseases produced by
toxic germs.
One of the special objects of my invention is to prepare nuclein from the different
organs and parts of the animal, in a perfectly natural condition, without the use of
deteriorating chemical agents, and so as to preserve the cellular activity and function
of the nuclein. After the nuclein has been prepared from the lymphoid struc-
tures of the body, preferably in a dry powdered state, its cellular activity is
preserved by investment or coating of the finely divided particles or grains, with
a solution of a suitable gum as benzoin.
It has been ascertained by comparatively recent investigation and tests, that
nuclein is the physical basis of all vital action, of tissue-growth and development.
Nuclein is a granular substance produced by cell action from the proteids of the
circulation and is the product of the multi-nuclear white blood corpuscles or
When nuclein is formed
leucocytes. Its chemical formula is C,HNP₂O
49
in the blood, it is the result of the action upon proteids by the leucocyte. When
nuclein is introduced (through the mouth and stomach) into the organism, it acts
in two ways: First, it immediately excites the osmotic action of each leucocyte,
causing it to proliferate, which is lecocytosis, and also to break into nuclei. The
more active the leucocytosis, the greater the vital resistance to toxic disease germs.
Second, it furnishes ready made to the leucocyte, material, which, under a de-
pressed vital condition, it is unable of itself to construct from the proteids, and thus
lifts the organism to the normal physiological standard or state of perfect health,
while it so strengthens and increases the leucocytes that they overpower the toxic
germs with which they come in contact. Every leucocyte, if in full physiological
activity, has the power of taking up the toxic germs and destroying their vitality.
Anything, therefore, which excites the growth of leucocytes to that extent, tends to
eliminate disease from the organism.
Immediately upon the formation of nuclein in the leucocyte and while it remains
within its walls, it is the same wherever found, but when delivered up to the
individual tissue cell, it takes at once the molecular impress of that cell, only that

page_text: portion being appropriated which is necessary for the tissue building purposes
over which such cell presides. In this way, we have nuclein modified by each
cell. A combination of nuclein from the various tissues of the body, produces my
composition which is termed "Protonuclein."
My protonuclein composition is prepared from the thyroid and thymus glands,
brain, (pineal glands and pituitary body) bone-marrow, pancreas, spleen, liver,
salivery glands, Brunner's glands, Lieberkuhn's follicles, and peptic glands. In
the preparation of nuclein from the above-mentioned glandular bodies, care is taken
to preserve the free leucocytes which are charged with basic nuclein.
Protonuclein is an exact physiological product derived directly from the lymphoid
structures of the body without the use of chemical agents. It is the simple dried
and preserved cellular nuclein elements of the organism. So delicate is proto-
nuclein that any chemical agent is liable to disturb its cellular activity.
The process of preparing protonuclein is preferably conducted as follows: The
thyroid and thymus glands, brain, bone marrow, pancreas, spleen, liver, salivary
glands, Brunner's glands, Lieberkuhn's follicles, and peptic glands should be
removed soon as possible after the animal has been slaughtered and then the
lymphoid tissues, which contain the largest amount of nuclein, are carefully
dissected from the coarser, fleshy, or muscular tissues and fat, which are rejected,
the glandular organs, being at the same time, kept in neutral condition. The
lymphoid tissues thus obtained are thoroughly dried at a temperature below that
which would coagulate the albuminous matter preferably 100° to 110° F., and not
exceeding 130° F., and then reduced to a coarse powder. This powder may be
treated with ether or other suitable solvent to remove the fat, after which the powder
is again dried, preferably at a temperature of 100° F. to 110° F., and not exceeding
130° F., and is then reduced to a fine powder. The cells containing the nuclein
being dry, hard, and brittle, are readily powdered, while the epidermal tissue or
skin and some muscular fibre remain in the form of thin scales or films which are
readily separated by a sieve. A sieve with a moderately fine mesh is used. The
fine powder contains the nuclein cells of all the organs which have been treated.
In order to preserve the cellular activity of nuclein prepared as above described,
the powder is treated with a dilute ethereal solution of a suitable gum, as benzoin,
so as to invest the finely divided particles or granules with a protective coating.
The benzoin is inert so that it does not affect the nuclein except to preserve it.
The benzoinized powder is then spread out and allowed to dry, and the odor of
ether thus expelled. The prepared powder may then be mixed with milk-sugar
in suitable proportion and formed into tablets or put up in powdered condition,
or it may be treated with glycerine and other solvents and made into a solution
and used as occasion requires.
The powdered nuclein of the different glands are preferably mixed in such pro-
portion as they are obtained from each organ; that is, the yield of ten or one
hundred of each of the glands are mixed together and then mixed with a suitable
proportion of milk-sugar. The milk-sugar assists in preserving the neuclein, and
maintaining its cellular activity and vitalizing functions in unimpaired condition.
Nuclein obtained from each of the above-mentioned organs differs materially
in its nutritive and tissue-building properties, and a mixture, as above indicated,
is found most useful in causing healthy assimilation, and repair and growth of
the different structures of the human organism. Nuclein also possesses peculiar
properties in destroying toxic germs. A mixture of nuclein prepared from the
different above-mentioned sources is, therefore, most effective in combating disease
germs, and poisonous ptomaines, and in acting as an efficient tissue builder.
Since protonuclein rapidly restores the vitality of all the tissues by aiding and
supporting assimilative nutrition, it may be administered with advantage in cases
of neurasthenia, exhaustion from business care, wasting diseases, pernicious
anaemia, marasmus, general weakness, and all conditions in which the organism
is below the normal physiological standard.
As an antitoxic agent it should be administered in all diseases depending upon

page_text: a toxic germ, especially in diphtheria, typhoid fever, scarlet fever, cholera infantum,
dyspepsia, tuberculosis, etc." If administered to persons who are subjected to
infectious diseases, it will act as a reliable preventive, either granting absolute
immunity from the disease or so modifying the attack of the disease as to destroy
its virulence.
Protonuclein may also be administered with advantage in place of what are
known as
"culture of disease germs," which are now often injected into the cir-
culation to combat other disease germs already in the system. It will accomplish
the same end and be more acceptable to the organism, free from the danger of
introducing additional, poisonous elements which frequently lead to fatal results.
Nuclein or protonuclein can be taken into the organism directly through the natural
channels, as digestive agents do not affect it and thus the necessity of introduction
directly into the circulation is avoided.
Having now particularly described and ascertained the nature of my said
invention and in what manner the same is to be performed, I declare that what
I claim is
1, The above described protonuclein composition containing cellular nuclein
possessing its normal cellular activity, having nutritive and antitoxic properties,
and in dry, powdered form, capable of preservation, substantially as described.
2 The above described protonuclein composition containing cellular nuclein,
derived from different glands and organs of the animal organism, possessing its
normal cellular activity, having nutritive and antitoxic properties, and in dry,
powdered form, capable of preservation, substantially as described.
A nutritive and antitoxic preparation containing the cellular elements or
nuclein, possessing its normal cellular activity, in a dry powdered form, the particles'
or granules of which are coated or invested with a suitable gum, as benzoin,
for preserving it.
4, As an article of manufacture a nutritive and antitoxic preparation containing
cellular nuclein in dry powdered form, capable of preservation, and milk-sugar.
5.A nutritive and antitoxic preparation containing cellular nuclein in dry
powdered form, the particles or granules being coated with a suitable gum for pre-
serving its cellular activity and milk-sugar.
6,The process of preparing a nutritive and antitoxic substance containing
nuclein from different animal organs which consists in dissecting the lymphoid
tissue containing the largest percentage of nuclein from the muscular and other
coarse tissues, drying the lymphoid tissues at a temperature below that which would
coagulate the albuminous matter, then reducing it to a powder and separating the
nuclein cellular matter from the scaly and fibrous matter, as by sieving.
The process of preparing a nutritive and antitoxic substance containing
nuclein from different animal organs which consists in dissecting the lymphoid
tissues containing the largest percentage of nuclein from the muscular and other
coarse tissues, drying the lymphoid tissues at a comparatively low temperature,
not exceeding 130° F., then reducing it to a powder and separating the nuclein
cellular matter from the scaly fibrous matter by sieving or other means, then
treating the nuclein cellular powder with a solution of a suitable gum, as benzoin
for protecting and preserving it as described.
HASELTINE, LAKE & Co.,
45 Southampton Buildings, London, W.C., Agents for the Applicant.