Manufacture of Peptones Free from Albuminous Matters.
Year: 1892

page_text: Date of Application, 7th Dec., 1892
Complete Specification Left, 2nd Sept., 1893—Accepted, 14th Oct., 1893
PROVISIONAL SPECIFICATION.
[Communicated from abroad by THE FARBWERKE vormals MEISTER LUCIUS
AND BRUNING, of Hoechst on Maine, in the German Empire, Chemical
Manufacturers.]
Manufacture of Peptones Free from Albuminous Matters.
I, OLIVER IMRAY, of No. 28 Southampton Buildings, Chancery Lane, in the
County of London, Chartered Patent Agent, do hereby declare the nature of this
invention to be as follows:-
Hitherto no process has been published for the manufacture of peptones free from
albuminous matters.
The so called peptones of commerce are all mixtures of albuminous matters and
peptones in various proportions.
For separating albuminous matters from peptones a method has been employed
which is based upon the capability of albuminous matters being precipitated by
sulphate of ammonia; but the desired result can only be attained under certain
conditions hereafter set forth.
The mixture of albuminous bodies with peptones prepared from albumen materials
of any quality and origin by enzymes, acids, alkalies, and overheating with water
cannot by precipitating with sulphate of ammonia be entirely freed from the
albuminous matters unless the saturation with the salt is proceeded with
1) In dilute solution
2) Successively in neutral, alkaline and acid solutions
3) At boiling heat with subsequent energetic refrigeration; in this operation,
while the purification is proceeding, the albuminous matters are separated the
peptones remaining in solution. The peptones are separated by crystallising out
the sulphate of ammonia, removing the residue of the salt by alcohol and cooling;
finally by treatment with carbonate of barium with addition of ammonia and
carbonate of ammonia, by which treatment the barium peptonate is at once set
free from the barium. This latter effect may also be produced by precipitation of
the barium by means of sulphuric acid.
The peptone can be farther purified by repeated precipitation with alcohol but
only when a very small quantity of sodium chloride or of other soluble salts is
added, as otherwise no precipitates capable of filtration or self deposition will be
formed.
EXAMPLES.
5 kg. of washed and pressed blood fibrin together with the pepsin obtained from

page_text: in treating with pepsin and hydrochloric acid to add a surplus of from 40 to 50 gr.
of hydrochloric acid for every kg. of commercial peptone.
Dated this 7th day of December 1892.
ABEL & IMRAY,
Agents for the Applicant.
COMPLETE SPECIFICATION.
Manufacture of Peptones Free from Albuminous Matters.
I, OLIVER IMRAY, of No. 28 Southampton Buildings, Chancery Lane, in the
County of London, Chartered Patent Agent, do hereby declare the nature of this
invention and in what manner the same is to be performed to be particularly
described and ascertained in and by the following statement:-
Hitherto no process has been published for the manufacture of peptones free
from albuminous matters.
The so called peptones of commerce are all mixtures of albuminous matters and
peptones in various proportions.
For separating albuminous matters from peptones a method has been employed
which is based upon the capability of albuminous matters of being precipitated by
sulphate of ammonia; but the desired result can only be attained under certain
conditions hereafter set forth.
The mixture of albuminous bodies with peptones prepared from albumen materials
of any quality and origin by enzymes, acids, alkalies, and overheating with water
cannot by precipitating with sulphate of ammonia be entirely freed from the
albuminous matters unless the saturation with the salt is proceeded with first in
dilute solution, secondly in neutral, alkaline and acid solutions successively, and
thirdly at boiling heat with subsequent energetic refrigeration; in this operation,
while the purification is proceeding, the albuminous matters are separated, the
peptones remaining in solution. The peptones are separated by crystallising out
the sulphate of ammonia, removing the residue of the salt by alcohol and cooling;
finally by treatment with carbonate of barium with addition of ammonia and
carbonate of ammonia, by which treatment the barium peptonate is at once freed
from barium. This latter effect may also be produced by precipitation of the barium
by means of sulphuric acid.
The peptone can be further purified by repeated precipitation with alcohol but
only when a very small quantity of sodium chloride or of other soluble salts is
added, as otherwise no precipitates capable of filtration or self deposition will be
formed.
EXAMPLES.
5 kilos. of washed and pressed blood fibrin together with the pepsin obtained
from 750 grammes of mucous membrane of the stomach and 15 litres of hydrochloric
acid of 0.4 per cent. are kept at 40° C. for a fortnight, then neutralised by carbonate
of soda, filtered off, slightly acidulated with acetic acid, boiled, and the solution
filtered again, is submitted to the treatment described; or
5 kilos. of such fibrin together with 5 litres of slightly alkaline trypsin solution,
1.5 11

page_text: necessary in treating with pepsin and hydrochloric acid to add a surplus of from
40 to 50 grammes of hydrochloric acid for every kilo. of commercial peptone.
Having now particularly described and ascertained the nature of my said inven-
tion and in what manner the same is to be performed I declare that what I
claim is :-
1. The herein described manufacture of peptone free from albuminous matters,
consisting in separating albuminous matters from their mixture with peptones by
treating the mixture with sulphate of ammonia first in dilute solution, secondly in
neutral alkaline and acid solution successively, and thirdly at boiling heat with
subsequent refrigeration.
2. Peptones freed from albuminous matters in the manner above referred to.
Dated this 2nd day of September 1893.
ABEL & IMRAY,
Agents for the Applicant.