Manufacture of Preparations of Iron and Albumen.
Year: 1898

page_text: PROVISIONAL SPECIFICATION.
Manufacture of Preparations of Iron and Albumen.
I, CHARLES DENTON ABEL, of Birkbeck Bank Chambers, Southampton Buildings,
Chancery Lane, in the County of London, Consulting Engineer and Chartered Patent
Agent, do hereby declare the nature of this invention (which has been communicated
to me from abroad by Anilinölfabrik A. Wülüing, of Elberfeld, in the Empire of
Germany) to be as follows:-
The present invention is based on the fact that the iron-compounds or salts of
certain nitroso-derivates, especially of the nitrosonaphtholsulpho acids, the nitroso-
oxycarbonic acids, the nitrosodioxynaphthalines, the dinitrosodioxybenzols and the
dinitroso-dioxynaphthalines, which in this fortn or in the form of their alkaline salts
are soluble in water, can be brought to a form such as to be insoluble in water,
and in dilute acids, but easily soluble in the alkaline salts, if combined with certain
albumen derivatives.
Thus, for example, the iron-salt of a-nitroso-b naphtholmonosulpho acid
(naphthol green) is easily soluble in water, whether the latter has been acidified or
is alkaline. But if one mixes a solution of these iron-compounds with the
solution of a certain amount of albumen in water and the liquid mixture is heated
to 90° C. after having acidified the latter, for example with acetic acid, a
coagulation of the albumen takes place whereby a certain portion of the iron
compound of the nitrosonaphtholsulpho acid becomes combined with the albumen
and is retained with great force by the latter so that the iron compound (the
naphthol-green) can only be separated by water from the dried up and powdered
derivative of iron and albumen in traces, but cannot be separated at all by dilute
acids, for example, 0-2 to 0.3 p.c. of hydrochloric acid. On the other hand it is very
easy to separate the iron compounds of the nitroso-naphtholsulpho acid from the
coagulated albumen by means of a solution of an alkaline salt.
It is to be observed that the other compounds of albumen with the
above-named iron nitroso-derivatives possess similar properties to those described
with regard to the albumen compound of the iron salt of nitrosonaphtholsulpho acid,
mentioned above.
From the above the following very important practical result is obtained.
As the said substances are intended to afford the human body the iron of which the
latter may be deficient, in such a form as to be easily absorbed, but which iron in the
ordinary form of salts is soluble in water and therefore easily corrodes the teeth and
the mucous membrane of the stomach, this noxious property of the said iron-salts
can be eliminated by simply bringing them into combination with albuminous
compounds, because the derivatives of iron and albumen are insoluble in water and in
liquids containing a small amount of acid; therefore, they neither attack or corrode
the teeth nor the mucous membrane of the stomach. Furthermore, the said
derivatives become, as before, easily dissolved in the intestinal liquids which are very
alkaline, so that the iron in the digestive canal will be in such a form as to become
easily absorbed.

page_text: The method of preparing the above-named derivatives of iron and albumen is the
following
--
About 100 grammes of albumen are mixed with about 1 litre of cold water and
then so much warm water is added that the temperature of the liquid reaches 45° C.
At the
The mixture is well stirred up until the whole of the albumen is dissolved.
same time about 12.5 graines of an iron compound of the a-nitroso-b-naphthol-
monosulpho acid salt of sodium (naphthol-green) is dissolved in a small quantity of
warm water and both solutions are then united. The mixture is then acidified with
about 25 cube cm. of acetic acid of 50% and the whole is heated to 90° C. During
this heating a green flocky precipitate is formed, which is separated from the liquid
by filtration. The precipitate, after having been well pressed out, is poured into
about 2 litres of boiling water and is allowed to cool down. The green precipitate is
then separated by filtration, the almost colourless liquid which adheres to the pre-
cipitate is pressed out and the body obtained after such pressing out is dried at a
temperature of from 90-100° C. The product is then finely ground and is
obtained in the form of a light-green powder possessing neither odour nor flavour.
Instead of using the naphthol green for the above purpose, one can also use with
equal success the iron compounds or salts of the other nitrosonaphtholsulpho acids, of
the nitrosooxycarbonic acids, of the nitrosodioxynapthalines, of the dinitrosodioxy-
benzols and of the dinitrosodioxynaphthalines.
Furthermore, instead of using the albumen as such is contained in eggs, one can
also use casein as well as other albuminous bodies or albumenose.
The mode of preparing the derivates of iron and albumen must be correspondingly
altered and is then as follows:-
1. 100 grammes of caseine as contained in milk, (said caseine being pure and free
from fatty admixtures) are dissolved in water by adding thereto the necessary
quantity of an alkaline salt. To this mixture are added 12.5 gr. of naphthol-green,
and the whole is well stirred up and the mixture is acidified with a small quantity
of acetic acid. The acidified mass is then heated in an autoclave to from
130 to 140° C. whereby the caseine becomes coagulated. After the mass has been
cooled down, the green-derivative of iron and albumen is separated by filtration and
the product obtained in this way is subjected to the same further inanipulations as
that previously described.
2. About 50 gr. of an albuminous body such as somatose is dissolved in about
750 cube cm. of warm water and this solution is mixed with a solution of 6 gr. of the
iron compound of the a-nitroso-b-naphthol monosulpho acid salt of sodium (naphthol-
green) in water. The combined mixture is then heated to 50-60° C. and there
is added to the mass about 60 cube cm. of acetic acid of 50%. A green and caseous
precipitate is formed, which separates from the mass as soon as the latter is cooled
down. The further treatment of this precipitate is as follows:-The mass is filtered,
washed out with a small quantity of cold water, is then pressed out and dried at
a low temperature such as 60 to 70° C. The product, after having been ground,
appears as a light-green powder.
Dated this 10th day of Wahrmory 1808
I, CHARLES DENTON ABEL, of Birbeck Bank Chambers, Southampton Buildings,
Chancery Lane, in the County of London, Consulting Engineer and Chartered Patent 5
Agent, do hereby declare the nature of this invention (which has been communicated

page_text: to me from abroad by Anilinölfabrik A. Wulfing, of Elberfeld, in the Empire of
Germany) and in what manner the same is to be performed to be particularly
described and ascertained in and by the following statement:-
The present invention is based on the fact that the iron-compounds or salts of
certain nitroso-derivates, especially of the nitrosonaphtholsulphonic acids, the nitroso-
oxycarboxylic acids, the nitrosodioxynaphthalenes, the dinitrosodioxybenzenes, and
the dinitrosodioxynaphthalenes, which in this form or in the form of their alkali
salts are soluble in water, can be brought to a form such as to be insoluble in water,
and in dilute acids, but easily soluble in the alkaline salts, if combined with certain
albumen derivatives.
Thus, for example, the iron compound of sodium-a-nitroso-ẞ-naphthol monosulphonate
(naphthol green) is easily soluble in water, whether the latter has been acidified or
is alkaline. But if one mixes a solution of these iron-compounds with the solution of
a certain amount of albumen in water, acidifies the liquid mixture, for example with
acetic acid, and heats it to 90° C., a coagulation of the albumen takes place whereby
a certain portion of the iron compound of the nitrosonaphtholsulphonic acid becomes
combined with the albumen and is retained with great force by the latter, so that
the iron compound (the naphthol green) can only be separated by water from the
dried up and powdered derivative of iron and albumen in traces, but cannot be
separated at all by dilute acids, for example, one containing 0.2 to 0-3 p.c. of hydro-
chloric acid. On the other hand it is very easy to separate the iron compounds of
the nitrosonaphtholsulphonic acid from the coagulated albumen by means of a solution
of an alkaline salt.
It is to be observed that the other compounds of albumens with the abovenamed
irou nitroso-derivatives possess similar properties to those described with regard to
the albumen compound of the iron salt of nitrosonaphtholsulphonic acid, mentioned
above.
From the above the following very important practical result is obtained.
As the said substances are intended to afford the human body the iron of which
the latter may be deficient, in such a form as to be easily absorbed but which iron
in the ordinary form of salts is soluble in water and therefore easily corrodes the
teeth and the mucous membrane of the stomach, this noxious property of the said
iron-salts can be eliminated by simply bringing them into combination with
albuminous compounds, because the derivatives of iron and albumen are insoluble in
water and in liquids containing a sinall amount of acid; therefore, they neither
attack nor corrode the teeth, or the mucous membrane of the stomach. Further-
more, the said derivatives become, as before, easily dissolved in the intestinal liquids
which are very alkaline, so that the iron in the digestive canal will be in such a form
as to become easily absorbed.
The method of preparing the abovenamed derivatives of iron and albumen is the
following:-
About 100 grammes of albumen are mixed with about one litre of cold water and
then so much warm water is added that the temperature of the liquid is raised to
45° C. The mixture is well stirred until the whole of the albumen is dissolved, at
the same time about 12.5 grammes of an iron compound of the sodium a-nitroso-ẞ-
naphtholmonosulphonate (naphthol-green) is dissolved in a small quantity of warm
water and both solutions are then mixed. The mixture is then acidified with about
25 cube c.m. of acetic acid of 50 per cent. strength and the whole is heated to 90° C.
During this heating a green flocculent precipitate is formed, which is separated from
the liquid by filtration. The precipitate, after having been well pressed, is poured
into about 2 litres of boiling water and is allowed to cool down. The green precipitate
is then separated by filtration, the almost colourless liquid which adheres to the
precipitate is pressed out and the body obtained after such pressing is dried at a
temperature of from 90° to 100° C. The product is then finely ground and is
obtained in the form of a light-green powder possessing neither odour nor flavour.
Instead of using the naphthol green for the above purpose, one can also use with
equal success the iron compounds or salts of the other nitrosonaphtholsulphonic acids,

page_text: of the nitroso-oxycarboxylic acids, of the nitroso-dioxynaphthalenes, of the dinitroso-
dioxybenzenes and of the dinitroso-dioxynaphthalenes.
Furthermore, instead of using the albumen as such is contained in eggs, one can
also use casein as well as other albuminous bodies or albumose.
The mode of preparing the derivates of iron and albumen must be correspondingly
altered and is then as follows:-
1. 100 grammes of casein as contained in milk, (said casein being pure and free
from fatty admixtures) are dissolved in water by adding thereto the necessary quantity
of an alkaline salt. To this mixture are added 12.5 gr. of naphthol-green, and the
whole is well stirred up and the mixture is acidified with a small quantity of acetic
acid. The acidified mass is then heated in an autoclave to from 130 to 140° C.
whereby the casein becomes coagulated. After the mass has been cooled down, the
green-derivative of iron and albumen is separated by filtration and the product
obtained in this way is subjected to the same further manipulation as that previously
described.
2. About 50 grams of an albumose, such as the albumose product commercially
known as somatose, is dissolved in about 750 cubic cm. of warm water and this solu
tion is mixed with a solution of 6 grams of the iron compound of sodium a-nitroso-ẞ-
naphtholmonosulphonate (naphthol-green) in water. The combined mixture is then
heated to from 50° to 60° C. and there is added to the mass about 60 cubic cm. of
acetic acid of 50 per cent. strength. A green and caseous precipitate is formed,
which separates from the mass as soon as the latter is cooled down. The further
treatment of this precipitate is as follows:-The mass is filtered, washed with a small
quantity of cold water, is then pressed and dried at a low temperature such as from
60° to 70° C. The product, after having been ground, is a light green powder.
Having now particularly described and ascertained the nature of this invention and
in what manner the same is to be performed, I declare that what I claim is:
1. The herein described manufacture of preparations of iron and albumen by
coagulating albuminous bodies in presence of acid and of an iron compound of a
nitrosonaphthol monosulphonic acid, a nitroso-naphtholdisulphonic acid, a nitroso-
naphtholtrisulphonic acid, a nitroso-oxycarboxylic acid, a nitrosodioxynaphthalene,
a dinitrosodioxybenzene or a dinitrosodioxynaphthalene.
2. Preparations of iron and albumen manufactured as above set forth.
Dated this 3rd day of November 1898