Improvements relating to the Preparation of Hydrochlorides, Hydrobromides, or Hydriodides of Peptone, and Double Salts thereof.
Year: 1890

page_text: Date of Application, 17th July, 1890
Complete Specification Left, 1st Apr., 1891-Accepted, 23rd May, 1891
PROVISIONAL SPECIFICATION.
[Communicated from abroad by KALLE & Co., of Biebrich on the Rhine, in the
Empire of Germany, Manufacturers.]
Improvements relating to the Preparation of Hydrochlorides,
Hydrobromides, or Hydriodides of Peptone, and Double Salts
thereof.
I WILLIAM ROBERT LAKE, of the firm of Haseltine, Lake & Co. Patent
Agents 45 Southampton Buildings, in the County of Middlesex, do hereby declare
the nature of this invention to be as follows:-
:-
This invention has for its object the preparation of hydrochlorides, hydro-
bromides and hydriodides of peptones, which are characterized by their solubility
in alcohol and of double salts of the same with metallic chlorides specially with
chloride of mercury and chloride of iron. These salts are intended for medical
tises.
The hydrochlorides, hydrobromides, and hydriodides of peptones are obtained
by treating ordinary peptones or albuminoid matters like gelatin, albumen, casein
and serum with hydrochloric, hydrobromic or hydriodic acid, dissolving the
products of the reaction in alcohol and evaporating the solution to dryness in a
vacuum.
The different raw materials yield salts having the same properties and also the
same composition.
If the free peptones are to be separated for example by precipitating the
aqueous solution of the salts with phospho-molibdic acid and decomposing the
precipitate with baryta, the products thus obtained appear to be in every respect
identical with the peptones obtained by the artificial digestion of albuminous and
peptic substances. By treatment with halogen-acids in alcohol they give at first
insoluble salts which become soluble when heated with the acids for some hours.
The cold aqueous solutions of the new salts give the reaction of biuret and are
precipitated by phospho-molybdic acid, phospho-wolframic acid and the double salt
of mercuric iodide with iodide of potassium.
They are now precipitated by tannic acid, by a concentrated aqueous solution of
sulphate of ammonia and by potassium ferrocyanide and acetic acid. Chloride of
mercury precipitates the hydriodides only, giving reddish yellow bodies very
insoluble in alcohol and water.
The process by which I prepare the said salts of peptones by means of the
reaction hereinbefore mentioned, is explained by the following examples.
I. HALOGEN SALTS OF GELATIN PEPTone.
To prepare hydrochloride of gelatin peptone I take 50 parts of gelatin, 25 parts
of hydrochloric acid (containing 37 per cent. of HCI) and 80 parts of water and
1 evaporate the mixture on a water-bath till the product is almost perfectly soluble
in alcohol.
then allow it to anal dissolus the sun in about 250 marts of absolute alcohol

page_text: obtain a product almost perfectly soluble in alcohol, the amount of HCl increases
to 15 per cent.
The hydrobromides and hydriodides of gelatin-peptone are produced in the same
manner by substituting hydrobromić and hydriodic acid by hydrochloric acid.
II. HALOGEN SALTS OF ALBUMEN PEPTONES.
If albuminoid matters be heated with mineral acids, the mixture readily assumes
an intense violet or black colour which is produced by the action of the oxygen of
the air. Nevertheless only small quantities of colouring matters are formed.
This colouring does not take place in the presence of reducing agents like
hydriodic acid.
To produce hydrochlorides of albumen peptones 100 parts of albumen, 80 parts
hydrochloric acid (containing 37 parts of HCI) and 20 parts of water are heated
for about three hours on a water-bath and constantly stirred.
The product of the reaction is then diluted with water, saturated with carbonate
of lead, precipitated with sulphuretted hydrogen and the liquid separated by
filtration of the sulphur of lead and the other impurities is evaporated on a water-
bath to a syrup, which is dissolved in alcohol by adding small quantities of hydro-
chloric acid. I then evaporate the alcohol in a vacuum substantially in the manuer
before described.
To obtain the hydriodides of albumen peptones the treatment with carbonate of
lead can be dispensed with.
The other raw materials are treated in the same manner.
III. DOUBLE SALTS OF HYDROCHLORIDES OF PEPTONES WITH CHLORIDE
OF MERCURY.
By adding alcoholic solutions of chloride of mercury and of hydrochloride of
gelatin-peptone double salts are obtained the one very insoluble in alcohol con-
taining as a rule 50 per cent. chloride of mercury the other containing 35-45 per
cent. of chloride of mercury and soluble in alcohol, this solution being precipitated
by means of ether. Both salts may be employed in practice, so that the separation
thereof is not necessary.
To prepare a product fitted for medical use, I dissolve 3 parts of hydrochloride
of peptone and 1 part of chloride of mercury in a small quantity of water and I
evaporate the solution in a vacuum after having added alcohol to it till the salt
becomes solidified.
IV. DOUBLE SALTS OF HYDROCHLORIDES OF PEPTONES WITH CHLORIDE
OF IRON.
The aqueous or alcoholic solutions of hydrochlorides of peptones are not pre-
cipitated by means of chloride of iron.
To obtain a product fitted for medical use an alcoholic solution of 9 parts of
hydrochloride of peptone and of 1 part of chloride of iron is evaporated in a
vacuum in a water-bath to dryness. The double salt constitutes yellowish, hygro-
scopical lamellae, very easily soluble in water and alcohol.
Dated this 17th day of July 1890.
HASELTINE, LAKE & Co.,
45, Southampton Buildings, London, Agents for the Applicant.

page_text: COMPLETE SPECIFICATION.
Improvements relating to the Preparation of Hydrochlorides,
Hydrobromides, or Hydriodides of Peptone, and Double Salts
thereof.
I, WILLIAM ROBERT LAKE of the firm of Haseltine Lake & Co. Patent Agents,
45 Southampton Buildings in the County of Middlesex do hereby declare the nature
of this invention and in what manner the same is to be performed, to be particularly
described and ascertained in and by the following statement:-
This invention has for its object the preparation of hydrochlorides, hydrobromides
and hydriodides of peptones, which are characterized by their solubility in
alcohol, and of their double salts with metallic chlorides, especially with mercuric
chloride and chloride of iron. These salts are intended for medical purposes.
The hydrochlorides, hydrobromides and hydriodides of peptones are obtained by
treating ordinary peptones or albuminoid matters like gelatine, albumen, casein and
serum with hydrochloric, hydrobromic or hydriodic acid, dissolving the products of
the reaction in alcohol and evaporating the solution in vacuo to dryness.
The different raw materials yield salts having the same properties and almost the
same composition. If the free peptones are to be separated, for example by
precipitating the aqueous solution of the salts with phospho-molybdic acid and
decomposing the precipitate with baryta, the products thus obtained appear to be
in every respect identical with the peptones obtained by the artificial digestion of
albuminous and pepsin substances. They give by treatment with halogen acids at
first products insoluble in alcohol, which become soluble when heated with the acids
for some hours.
The cold aqueous solutions of the new salts give the reaction of biuret and are
precipitated by phospho-molybdic acid, phospho-wolframic acid and the double salt
of mercuric iodide with iodide of potassium. They are not precipitated by tannic
acid, by a concentrated aqueous solution of sulphate of ammonia, or by potassium
ferrocyanide and acetic acid. Chloride of mercury precipitates the hydriodides only,
giving reddish-yellow bodies, very insoluble in alcohol and water.
The process by which I prepare the said salts of peptones by means of the
reaction herein before mentioned, I explain or illustrate by the following examples.
I. HALOGEN SALTS OF GELATINE PEPTONE.
To prepare hydrochloride of gelatine peptone I take 50 parts of gelatine,
25 parts of hydrochloric acid (cont. 37% of HCI) and 80 parts of water and I
heat the mixture on a water-bath until the product is almost perfectly soluble in
alcohol. I then allow it to cool, dissolve the mixture in about 250 parts of absolute
alcohol filter un from mineral matters and distil the alcohol in vacuo. If the

page_text: the air. Nevertheless only small quantities of colouring matters are formed.
This colouration does not take place in presence of reducing agents like hydriodic
acid.
To produce hydrochlorides of albumen peptones, 100 parts of albumen, 80 parts
of hydrochloric acid (cont. 37 % of HCI) and 20 parts of water are heated for
about three hours on a water-bath and continually stirred. The product of the
reaction is then diluted with water, saturated with carbonate of lead, precipitated
with sulphuretted hydrogen and the liquid separated by filtration from the sulphur
of lead and the other impurities, is evaporated on a water-bath to a syrup which is
dissolved in alcohol by adding small quantities of hydrochloric acid. I then
evaporate the alcohol in vacuo, substantially in the manner set forth. To obtain the
hydriodides of albumen peptones the treatment with carbonate of lead can be
avoided. The other raw materials are treated in the same manner.
III. DOUBLE SALTS OF HYDROCHLORIDES OF PEPTONES WITH MERCURIC
CHLORIDE,
By adding alcoholic solutions of mercuric chloride and of hydrochloride of
gelatine peptone two double salts are obtained, the one very insoluble in alcohol
and containing as a rule 50% of mercuric chloride, the other containing 35-45% of
mercuric chloride, soluble in alcohol, this solution being precipitated by means
of ether. Both salts may be employed in practice, so that their separation is not
necessary.
To prepare a product fitted for medical use I dissolve 3 parts of hydrochloride of
peptone and 1 part of chloride of mercury in a small quantity of water and I
evaporate the solution in vacuo, after having added alcohol to it, until the salt
becomes solidified.
IV. DOUBLE SALTS OF HYDROCHLORIDES OF PEPTONES WITH CHLORIDE
OF IRON.
The aqueous or alcoholic solutions of hydrochlorides of peptones are not
precipitated by means of chloride of iron.
To obtain a product fitted for medical use an alcoholic solution of 9 parts of
hydrochloride of gelatine peptone, and of 1 part of chloride of iron is evaporated
in vacuo on a water-bath to dryness. The double salt forms yellowish, hygroscopical
scales, very easily soluble in water and alcohol.
Having now particularly described and ascertained the nature of the said inven-
tion, and in what manner the same is to be performed, as communicated to me by
my foreign correspondents, I declare that what I claim is :-
The preparation of hydrochlorides, hydrobromides and hydriodides of peptones
characterized by their solubility in alcohol and of their double salts with metallic
chlorides, especially with chloride of mercury and chloride of iron.
Dated this 1st day of April 1891.
HASELTINE, LAKE & Co.,
45, Southampton Buildings, London, Agents for the Applicant.