Improvements in and connected with the Manufacture of Soluble. Products from Proteids.
Year: 1898

page_text: Date of Application, 20th June, 1898
Complete Specification Left, 3rd Mar., 1899-Accepted, 8th Apr., 1899
DARISTOV UT ÖDEGTUIGATION
Improvements in and connected with the Manufacture of Soluble.
Products from Proteids.
We, FRIEDRICH SILBERSTEIN and Dr. LEON LILIENFELD, trading as Dr. Lilien-
feld & Co., Chemical Manufacturers, of 20, Ottakringerstrasse, Vienna, Austria,
do hereby declare the nature of this invention to be as follows;-
This invention relates to a process for obtaining albuminous compounds soluble
in alcohol from nucleo-albumins and nucleo-proteids and to the conversion of
these bodies into their silver compounds.
In the case of several proteids, for instance the nucleohiston, the nucleo-proteid
of pus or putrid matter, the nucleic acid which is rich in phosphorus, but free
from albumen, passes over into the alkaline filtrate.
The following example will serve to explain the process.
For obtaining products soluble in alcohol, a nucleo-proteid, for instance
legumin, is treated with a five per cent. aqueous solution of potash or soda (potas-
sium or sodium lye) for several hours in a digester, and the mixture thus pro-
duced is clarified by filtration.
In certain cases the clarification and filtration may be facilitated by adding to
the mass a suitable precipitant of albumen, for instance chloride of iron, or
other metallic salts, or phenol, and subsequently filtering.
According to the nature of the alkali employed, the filtrate thus obtained con-
tains the potassium compound or the sodium compound of the albuminous
body in solution, which compounds are insoluble in alcohol, and may therefore
be precipitated by the addition of alcohol.
By further treatment with sulphuric (or other) acid they are décomposed into
the albuminous body and the corresponding sulphate.
The albuminous body obtained by one of the methods described above, is
soluble in aqueous alcohol, and, when in alkaline solution, may be converted
into compounds of silver soluble in water. The said albuminous body possesses
considerable nutritive value and may therefore be used for the preparation of
food.
DL: 00th day of June 1898
FAIRFAX & WETTER,
Agents, 37 and 39, Essex Street, Strand, London.
Improvements in and connected with the Manufacture of Soluble
Products from Proteids.
We, FRIEDRICH SILBERSTEIN and Dr. LEON LILIENFELD, trading as Dr. Lilien-
feld & Co., Chemical Manufacturers, of 20, Ottakringerstrasse, Vienna, Austria,
do hereby declare the nature of this invention and in what manner the same is to be

page_text: performed, to be particularly described and ascertained in and by the following
statement:
This invention relates to a process for obtaining albuminous compounds soluble
in alcohol from nucleo-albumins and nucleo-proteids, and to the conversion of
these bodies into their silver compounds.
The following example will serve to explain the process.
For obtaining products soluble in alcohol, a nucleo-proteid, for instance
legumin, is treated with a five per cent. aqueous solution of potash or soda (potas-
sium or sodium lye) in a digester for several hours, and the mixture thus pro-
duced is clarified by filtration.
In certain cases the clarification and filtration may be facilitated by adding to
the mass a substance capable of precipitating albumen, for instance chloride of
iron, or other metallic salts, or phenol, and subsequently filtering.
According to the nature of the alkali employed, the filtrate thus obtained con-
tains the potassium compound or the sodium compound of the albuminous
body in solution, which compounds, as is well-known, are insoluble in alcohol,
and may therefore be precipitated by the addition of alcohol.
By further treatment with sulphuric or other acid, the compound obtained is
decomposed into the corresponding sulphate and the albuminous body.
If an acid is added directly to the filtrate, the decomposition takes place imme-
diately, and the albuminous body is precipitated out of the filtrate.
It should be observed, that there are certain compounds belonging to the class
of nucleo-albumins and nucleo-proteids, which when treated in a manner analo-
gous to that described in the foregoing example, will not give the results stated
therein. For instance, in the case of nucleohiston, the nucleo-proteid of pus or
putrid matter, the nucleic acid which is rich in phosphorus, but free from
albumen, passes over into the alkaline filtrate.
The albuminous body obtained by one of the methods described above is free
from phosphorus, soluble in aqueous alcohol, and when in alcoholic solution may
be converted into compounds of silver soluble in water.
For this purpose the albuminous body obtained is dissolved in ammoniacal
ethyl-alcohol, and an alcoholic solution of silver nitrate is added to this solution.
Thereby the argento-albuminous compound is formed, slowly at a low tempera-
ture, and more quickly with the aid of heat, which compound according to the
quantity of water contained in the alcohol, either separates spontaneously in the
shape of thick flakes, or is precipitated by means of concentrated alcohol.
The product obtained as described is used as a pharmaceutical compound or
preparation.
Having now particularly described and ascertained the nature of our said
invention, and in what manner the same is to be performed, we declare that what
we claim is:-
1. The process for converting proteids into albuminous compounds soluble in
alcohol, which consists in digesting the proteids with alkalies, and either preci-
pitating the novel substance from the product of the reaction by the addition of
an acid, or first precipitating from the product of the reaction, the potassium
or sodium compound by the addition of alcohol, and then decomposing the said
compound by the addition of acid, substantially as described.
2. The conversion of the new product obtained according to Claim 1 into its
silver compounds, by treating the said product when dissolved in ammoniacal
alcohol with an alcoholic solution of silver nitrate, substantially as described.
Dated this 3rd day of March 1899
FAIRFAX & WETTER,
Agents, 37 and 39, Essex Street, Strand, London.
FAIRFAX & WETTER,
Agents, 37 and 39, Essex Street, Strand, London.