Process for the Production of Water-soluble Compounds of Caseine and of its Derivatives.
Year: 1898

page_text: Date of Application, 28th Jan., 1898-Accepted, 3rd Sept.,
1898
COMPLETE SPECIFICATION.
Process for the Production of Water-soluble Compounds of Caseine
and of its Derivatives.
We, MAXIMILIAN RIEGEL, Doctor of Philosophy, Chemist, and Apothecary, and
JOHANNES ADOLF ROSE, Apothecary, both of No. 23, Rohrer-gasse, Cologne, in
the Empire of Germany, do hereby declare the nature of this invention and in
what manner the same is to be performed, to be particularly described and ascer-
tained in and by the following statement:-
The soluble alkali compounds of caseine, that is to say, both the combinations
reacting neutral upon phenolphtaline, and the acid compounds principally in use
for dietetic purposes, have been hitherto produced by the action of aqueous or
alcoholic potash or sodium hydrate upon caseinc. The preparation of these com-
pounds, by means of solutions of the alkaline hydrates in alcohol, has however
the disadvantage that a molecule of water is always formed from the hydroxyl
of the alkaline hydrate and the hydrogen atom of the caseine. Consequently,
the result is, a sticky gluish-substance, which can only be freed from water by
prolonged heating.
The presence of the water is completely obviated if, instead of the alkaline
hydrate, a solution of metallic sodium or potassium in alcohol free from water be
employed.
After accurately determining the acidity of the perfectly dry caseine, or of
the derivative or substitution product thereof, by means of litmus paper, the
calculated quantity of metallic sodium or potassium is dissolved in the least
possible quantity of perfectly anhydrous ethyl or methyl alcohol, air being
excluded. After the evolution of hydrogen has ceased, the liquid is diluted with
several times its volume of ether free from water. The caseine, or the caseine deriva-
tive, in a finely pulverised state, is moistened with this solution, and the alcohol
and ether is expelled by heating to 80° C., or to a lower temperature, if in vacun.
In place of ethyl alcohol, anhydrous methyl alcohol may be used; in this case,
the temperature need only be carried to from 66° to 68°. The caseine salts thus
produced are of a pure white color, and are distinguished from those prepared
by means of the hydrates, by their complete solubility in water.
In a similar manner the calcium compounds of caseine or of its derivatives
may be obtained. The caseine, mixed with dry calcium chloride, in quantity
proportionate to the amount of alkali in the caseine sodium compound, is boiled
with alcohol in the presence of the necessary quantity of a solution, in alcohol,
of metal ethylate or methylate, till the alkali-alcoholate has been perfectly changed
into the calcium-alcoholate; whereupon, after washing out the sodium chloride,
the resultant calcium compound is dried.
Having now particularly described and ascertained the nature of our said inven-
tion and in what manner the same is to be performed, we declare that what we
claim is:-
1. The process for the production of water-soluble compounds of caseine and of
its derivatives or substitution products consisting in treating caseine or its sub-

page_text: stitution products or derivatives with alkali alcoholate, obtained by dissolving
the alkali-metal in perfectly anhydrous ethyl or methyl alcohol.
2. The process for the production of water-soluble compounds of caseine and of
its derivatives, or substitution products, consisting in treating a mixture of
caseine and calcium-chloride, with alkali-alcoholate obtained by dissolving the
alkali-metal in perfectly anhydrous ethyl or methyl alcohol, and removing the
resultant alkali-chloride by washing.
Dated this 28th day of January 1898.
NEWTON & SON,
Agents for the Applicants.