Year: 1886

page_text: Date of Application, 28th June, 1886.
Complete Left, 19th Mar., 1887.
Complete Accepted, 22nd Apr., 1887.
RESERVE
I WILHELM MERCK of 9 Rheinstrasse Darmstadt. German Empire, Manufacturer
do hereby declare the nature of this invention to be as follows :—
This invention refers to the production of peptone or the separation of the nucleine
from the so called nucleo-proteines, whereby I understand such albuminous substances
which in the peptonizing, whether in a neutral acid or alkaline solution form nucleine
and proteine. Such substances are found for instance in the liver, in the yolk of
egg,
and in milk.
In order to obtain the peptone or to eliminate the nucleine from the nucleo-
proteines for instance the caseine of milk I proceed as follows
The caseine is digested or treated with water or with a dilute acid as for instance
sulphuric acid, or a dilute alkali as for instance potash lye, under pressure and at a
heat of 150° to 170° C until it is all peptonized and the remainder only consists of
nucleine. The latter is removed by filtration and application of water or acid, direct
while applying an alkaline solution after previous neutralization.
The peptone containing filtrate may if it should contain appreciable quantities of
unaltered albumen be further digested. The lastly obtained solution contains caseine-
peptone. In order to recover this I evaporate or boil down the solution at a heat
of 35° to 40° C to a syrup consistency. The precipitate, if any, may be filtered off.
I neutralize the acid reacting syrup by means of an alkali for instance potash, and if
a precipitate should be produced thereby 1 filter it off. The filtrate is caseine peptone
which may be rendered into a dry powder in various ways. The syrup may be dried
at 35° to 40° C and pulverized or it may be precipitated by means of alcohol of
absolute strength, or it may be desiccated and turned into a powder by a current of
compressed air.
The nucleine may also be eliminated in the following manner. 1 digest the caseine
with dilute soda or other alkaline lye at 80° to 90° C until the nucleine precipitate
produced after neutralization with acid does not increase further. The nucleine is

page_text: filtered off and the peptone containing filtrate is neutralized and rendered into
peptone in the manner above described.
The elimination of the nucleine may also be effected by suspending the caseine in
water with or without admixture of a dilute alkali and adding a ferment or ferment
containing substance having a peptonizing action in an alkaline or neutral solution
and digesting at 40° C until the nucleine precipitate arising by neutralization does not
increase any further. The precipitate is filtered off and the peptone containing
filtrate is rendered into peptone according to one of the modes described.
The methods described with reference to caseine apply of course to all other nucleo-
proteines.
JENSEN & SON,
77, Chancery Lane, W.C.,
For the Applicant.

page_text: I, WILHELM MERCK of 9 Rheinstrasse Darmstadt German Empire Manufacturer
do hereby declare the nature of this invention and in what manner the same is to be
performed to be particularly described and ascertained in and by the following
statement:-
This invention refers to the production of peptone or the separation of the nucleine
from the so-called nucleo-proteines, whereby I understand such albuminous substances
which in the peptonizing whether in a neutral acid or alkaline solution from nucleine
and proteine. Such substances are found for instance in the liver, in the yolk of egg,
and in milk.
In order to obtain the peptone or to eliminate the nucleine from the nucleoprotüines
for instance the cascine of milk I proceed as follows:-
The caseine is digested or treated with water or with a dilute acid as for instance
sulphuric acid, or a dilute alkali as for instance potash lye, under pressure and at a
heat of 150° to 170° C until it is all peptonized and the remainder only consists of
nucleine. The latter is removed by filtration and application of water or acid, direct
while applying an alkaline solution after previous neutralization.
The peptone containing filtrate may if it should contain appreciable quantities of
unaltered albumen be further digested. The lastly obtained solution contains caseine-
peptone. In order to recover this, I evaporate or boil down the solution at a heat
of 35° to 40° C to a syrup consistency. The precipitate if any may be filtered off.
I neutralize the acid reacting syrup by means of an alkali for instance potash, and if
a precipitate should be produced thereby I filter it off. The filtrate is caseine-peptone
which may be rendered into a dry powder in various ways. The syrup may be dried
at 35° to 40° C and pulverized, or it may be precipitated by means of alcohol of
absolute strength, or it may be desiccated and turned into a powder by a current of
compressed air.
The nucleine may also be eliminated in the following manner. I digest the cascine
with dilute soda or other alkaline lye at 80° to 90° C until the nucleine precipitate
produced after neutralization with acid does not increase further. The nucleine is
filtered off, and the peptone containing filtrate is neutralized and rendered into peptone
in the manner above described.
The elimination of the nucleine may also be effected by suspending the caseine in
water with or without admixture of a dilute alkali and adding a ferment or ferment-
containing substance having a peptonizing action in an alkali or neutral solution and
digesting at 40° C until the nucleine precipitate, rising by neutralization, does not
increase any further. The precipitate is filtered off and the peptone containing
filtrate is rendered into peptone according to one of the modes described.
The methods described with reference to caseine apply of course to all other
uneloo-nrotäines

page_text: Having now particularly described and ascertained the nature of my said invention
and in what manner the same is to be performed 1 declare that what I claim is :-
First: In the production of Peptone from nucleoproteines the process of elimi-
nating the nucleine by digesting with water or dilute acid or dilute alkaline lye under
pressure and at a temperature of 150° to 170° C and filtering substantially as
described.
Second-In the production of peptone from nucleo-proteines the process of
eliminating the nucleine by digesting with dilute alkali lye at 80° to 90° C, then
neutralizing with acid and filtering the nucleine precipitate, substantially as described.
Third In the production of peptone from nucleoproteine the process of elimi-
nating the nucleine by digesting with a ferment or ferment-containing substance
acting in an alkaline or neutral solution, then neutralizing and filtering, substantially
as described.