Manufacture of a Preventative of Swine Fever.
Year: 1898

page_text: PROVISIONAL SPECIFICATION.
Manufacture of a Preventative of Swine Fever.
I, OLIVER IMRAY, of Birkbeck Bank Chambers, Southampton Buildings, Chan-
cery Lane, in the County of London, Chartered Patent Agent, do hereby declare
the nature of this invention (as communicated to me from abroad by Farbwerk
Friedrichsfeld Dr. Paul Remy, of Mannheim, in the German Empire) to be as
follows:
:-
A pure culture of splenetic fever is prepared from an animal which has died of
this disease by placing a small particle of the spleen in nutritive gelatine and
after a few days transferring some of the culture grown therein to gelatine by the
method of puncture; a small portion of this pure culture is introduced by means
of a sterilisel platinum needle into nutritive broth. The inoculated broth is kept
in the dark for four days at 36° C. and is then transferred to an apparatus in
which the splenetic fever bacilli which have developed in the broth are so far
attenuated that pigeons inoculated therewith do not die, while a small portion
injected under the skin of a white mouse produces death in 3 days at most.
The product thus obtained is next mixed with pure glycerine in the proportion
of 3 to 7; of this mixture 10 cubic centimeters injected under the skin of the
inner surface of the hinder thigh is a dose sufficient to prevent a pig from contract-
ing splenetic fever. The animal thus treated either shows, as a consequence, no
apparent change in its normal condition, or a slight reaction sets in, some marks
appearing distributed over the body of the animal after from 4 to 5 days and there
being a loss of appetite for a short time. These symptoms disappear after a few
days and after from 10 to 14 days the animal develops an immunity from splenetio
fever which protects it for a year.
manufacture of a Preventative of Swine Fever.
I, OLIVER IMRAY, of Birkbeck Bank Chambers, Southampton Buildings, Chan-
cery Lane, in the County of London, Chartered Patent Agent, do hereby declare
the nature of this invention and in what manner the same is to be performed, (as
communicated to me from abroad by Farbwerk Friedrichsfeld Dr. Paul Remy, of
Mannheim, in the German Empire), to be particularly described and ascertained
in and by the following statement:-
A pure culture of swine fever (swine erysipelas) is prepared from an animal

page_text: which has died of this disease by placing a small particle of the spleen in nutritive
gelatine and after a few days transferring some of the culture grown therein to
gelatine by the method of puncture. A small portion of this pure culture is
introduced by means of a sterilised platinum needle into nutritive broth.
The inoculated broth is kept in the dark for four days at 36° C. and is then
transferred to an apparatus in which the fever bacilli which have developed in
the broth are so far attenuated that pigeons inoculated therewith do not die, while
a small portion injected under the skin of a white mouse produces death in 3 days
at most.
The product thus obtained is next mixed with pure glycerine in the proportion
of 3 to 7 of this mixture 10 cubic centimetres injected under the skin of the inner
surface of the hinder thigh is a dose sufficient to prevent a pig from contracting
this form of fever. The animal thus treated either shows, as a consequence, no
apparent change in its normal condition, or a slight reaction sets in, some
urticaceous marks appearing distributed over the body of the animal after
from 4 to 5 days and there being a loss of appetite for a short time. These
symptoms disappear after a few days and after from 10 to 14 days the animal
develops an immunity from swine fever which protects it for a year.
Having now particularly described and ascertained the nature of this
invention and in what manner the same is to be performed, I declare that what
I claim is:-
1. The herein described manufacture of a preventative of swine fever by cultivat-
ing the bacilli from the spleen of an animal which has died of the disease, keeping
the culture in the dark at 36° C., suitably attenuating the culture and mixing it
with glycerine.
2. A preventative of swine fever manufactured as above set forth.