An Improved Process for Raising Permanent Pure Cultivations of Lactic Acid Bacteria.
Year: 1896

page_text: [Communicated from abroad by LUDWIG VON LORENTZ, of Hamburg, in the
Empire of Germany, Merchant.]
An Improved Process for Raising Permanent Pure Cultivations of
Lactic Acid Bacteria.
I, HENRY HARRIS LAKE, of the Firm of Haseltine, Lake & Co., Patent
Agents, 45 Southampton Buildings, in the County of Middlesex, do hereby
declare the nature of this invention to be as follows:-
For the purpose of obtaining pure cultivations of lactic acid bacteria, such as
are used for acidulating cream with a view to the preparation of butter, in such a
manner as to render cultivations permanent or viable, a pure cultivation of such
lactic acid bacteria is brought into the presence of a four per cent. aqueous solution
of milk-sugar, if it be desired to keep it in a liquid condition. The cultivation,
which should be located in a sterilized space or room, is in such space, filled, say,
into sterilized glasses of a capacity of about 250 grammes, and containing
5 grammes of milk-sugar, after which the glasses are sealed with sterilized
stoppers or corks. The milk-sugar will then keep up the cultivation by feeding
the bacteria.
For the purpose of obtaining the preparation in the condition of powder, which
will "keep," the fluid cultivation should, in a sterilized space also, be brought
into contact with a mixture of potato starch and milk-sugar. A good proportion
will prove to be 200 parts by weight of starch to every 50 parts by weight of
milk-sugar; and then, to every 250 parts by weight of this mixture, 75 parts
of fluid cultivation may be added, the whole being dried at a temperature of
30° Centigrade. The powdered material should be preserved in sterilized and
well-sealed glasses.
When it is desired to obtain a cultivation in a solid compact form, a solid
porous cake is prepared by mixing 60 parts of gypsum, 20 parts of potato-starch
and 20 parts of milk-sugar by the aid of distilled water. The cake is then
sterilized with about 75 grammes of a pure fluid cultivation which is prepared, as
above described, and poured over the cake, which is then dried at a temperature
of under 30° Centigrade, and preferably preserved in sterilized filtering paper.
1000
HASELTINE, LAKE & Co.,
45 Southampton Buildings, London, W.C., Agents for the Applicant.

page_text: COMPLETE SPECIFICATION.
An Improved Process for Raising Permanent Pure Cultivations of
Lactic Acid Bacteria.
I, HENRY HARRIS LAKE, of the Firm of Haseltine, Lake & Co., Patent
Agents, 45 Southampton Buildings in the County of Middlesex, do hereby
declare the nature of this invention and in what manner the same is to be
performed, to be particularly described and ascertained in and by the following
statement:-
The present invention relates to an improved process for raising or producing
permanent pure cultivations of lactic acid bacteria.
For the purpose of obtaining pure cultivations of lactic acid bacteria, such as
are used for acidulating cream with a view to the preparation of butter, in such a
manner as to render cultivations permanent or viable, a pure cultivation of such
lactic acid bacteria is brought into the presence of a solution of about four grammes
of milk-sugar in one hundred grammes of water, if it be desired to keep it in a
liquid condition. From this cultivation, (which may be hereinafter called a fluid
cultivation) quantities of 200 grammes mixed with 5 grammes of milk-sugar are
filled, say, into sterilized glasses of a capacity of about 0.00025 cubic meter
or 0.25 cubic decimeter, that is to say, a fourth of a liter, after which the glasses
are sealed with sterilized stoppers or corks. The milk-sugar will then keep up the
cultivation by feeding the bacteria.
For the purpose of obtaining the preparation in the condition of powder, which
will "keep," the fluid cultivation should,-in a sterilized space also, be brought
into contact with a mixture of potato starch and milk-sugar. A good proportion
will prove to be 200 parts by weight of starch to every 50 parts by weight of
milk-sugar; and then to every 250 parts by weight of this mixture, 75 parts of a
fluid cultivation as stated above may be added, the whole being dried at a
temperature of 30° Centigrade. The powdered material should be preserved in
sterilized and well-sealed glasses.
When it is desired to obtain a cultivation in a solid compact form, a solid
porous cake is prepared by mixing 60 parts of gypsum, 20 parts of potato-starch
and 20 parts of milk-sugar by the aid of distilled water. The cake thus obtained
is first dried and then sterilized by heating, whereupon it is cooled and impregnated,
while kept in a sterilized room with about 75 grammes of the fluid cultivation
prepared, as above described. The cake is then dried again at a temperature of
under 30° Centigrade, and preferably preserved in sterilized filtering paper.
Having now particularly described and ascertained the nature of my said inven-
tion, and in what manner the same is to be performed, as communicated to me by
my foreign correspondent, I declare that what I claim is:-
A process of producing permanent or viable pure cultivations of lactic acid
bacteria which consists in mixing and preserving in a sterilized space cultivations
of such bacteria with milk-sugar with an addition of potato-starch and gypsum,
in order to keep the said cultivation in a viable, fluid or solid form as an
acidulating agent for the preparation of butter, substantially as and for the purpose
set forth.
HASELTINE, LAKE & Co.,
45 Southampton Buildings, London, W.C., Agents for the Applicant.