Abstract:
A water soluble preservative admixture of biocidal compounds for addition to commercial use compositions at predetermined use levels, and uniformly distributed therein, to provide long-time synergistic biocidal activity against a wide range of fungi and both gram-negative and gram-positive bacteria, which comprises powders of 
     (a) one or more methylol compounds, or their equivalents, and 
     (b) iodopropynyl alcohol, or its ester, carbamate or ether derivative thereof, in a weight ratio of (a):(b) of 100:1 to 2000:1.

Description:
This is a division of application Ser. No. 08/234,089, filed Apr. 28, 1994, now U.S. Pat. No. 5,428,050 issued Jun. 27, 1993. 
    
    
     BACKGROUND OF THE INVENTION 
     1. Field of the Invention 
     This invention relates to a water soluble preservative admixture for addition to commercial use formulations to provide long time synergistic biocidal activity therein, and, more particularly, to admixtures of a methylol compound and an iodopropynyl compound, in predetermined weight ratios of 100:1 to 2000:1. 
     2. Description of the Prior Art 
     Combinations of antimicrobial agents have been developed in the prior art in order to: 
     (1) produce a biochemical synergism; 
     (2) broaden the antimicrobial spectrum of activity of each agent; 
     (3) increase water solubility for the admixture; 
     (4) minimize the toxicity or irritation of a given agent to the host; and 
     (5) minimize physical and chemical incompatibilities. 
     True biological synergism exists when two agents, when combined, require lesser amounts of the agents to bring about the same inhibitory or cidal effect than either single agent alone. While synergistic interaction for two or more antimicrobial agents does produce more than merely an additive effect in the resultant biological activity, in most cases the mechanism of such synergism remains a mystery. 
     M. Rosen et al., in U.S. Pat. No. 4,844,891, for example, described a preservative admixture of (a) a formaldehyde donor and (b) a halopropynyl compound, in a weight ratio of (a):(b) of 50:1 to 1:1, preferably 30:1 to 2:1, and, most preferably, 20:1 to 10:1, as providing fungicidal activity for 1-3 days in commercial use formulations. However, Rosen observed that when the ratio of (a):(b) in the concentrate exceeded 50:1 (System No. 16 in Table 1, a ratio of 73.33), the preservative composition was ineffective in providing biocidal protection in the use formulations. Thus a relatively large amount of the halopropynyl compound was required by Rosen to provide significant biocidal activity in the use composition. In such admixtures, although the formaldehyde donor is water soluble, the halopropynyl compound is substantially insoluble in water. Therefore it was difficult for Rosen to uniformly distribute his admixture throughout the use composition. 
     For these and other reasons, it is desired to provide a new and improved water soluble preservative admixture of such biocidal compounds which requires relatively little of the water insoluble and expensive halopropynyl compound, and that also provides effective synergistic protection in use formulations against a wide range of fungi and bacteria at different use levels over a long period of time. 
     A feature of the present invention is the provision of an admixture concentrate which is water soluble and which therefore can be uniformly distributed in use compositions at a predetermined use level. 
     Another feature herein is the provision of a preservative admixture which exhibits a long term synergistic biocidal activity against wide range of fungi and both gram-negative and gram-positive bacteria in the use compositions. 
     Still another feature of this invention is the provision of a water soluble preservative admixture for personal care compositions in the form of a solution, lotion, gel, emulsion, emulsifiable concentrate, suspension, slurry or cream. 
     These and other objects and features of the invention will be made apparent from the following more particular description of the invention. 
     SUMMARY OF THE INVENTION 
     What has been discovered is a water soluble preservative composition for addition to commercial use compositions at predetermined use levels, and uniformly distributed therein, which provides long term synergistic biocidal activity against a wide range of fungi and both gram-negative and gram-positive bacteria. The composition of the invention comprises an admixture of powders of 
     (a) a methylol compound, or their equivalent, and 
     (b) iodopropynyl alcohol, or its ester, carbamate or ether derivative thereof, 
     in a weight ratio of (a):(b) of 100:1 to 2000:1, preferably 200:1 to 500:1. 
     Commercial use compositions containing about 0.01 to 2% by weight of the composition of the invention also are provided therein. Such use compositions contain an iodopropynyl compound in an amount of about 0.5 to 10 ppm, to provide the desired antifungal activity, and a methylol compound, or equivalent thereof, in an amount of at least 250 ppm, to provide the desired antibacterial activity. 
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     The invention is based upon the discoveries that in a predetermined admixture of (a) a methylol compound, or its equivalent, and (b) an iodopropynyl compound: 
     (1) Iodopropynyl compounds are substantially water insoluble at weight ratios of (a):(b) of less than 100; accordingly at weight ratios below 100:1, it is difficult to uniformly distribute the iodopropynyl compound in aqueous use compositions, particularly in creams, gels and the like. In this invention, the admixtures are used at a weight ratio of (a):(b) of 100:1 to 2000:1, which are water soluble in all use compositions at conventional use levels. 
     (2) Effective synergistic biocidal activity is achieved for admixtures having a weight ratio of (a):(b) of 100:1 to 2000:1. Such admixtures have a Synergistic Index (SI) value approaching zero (maximum synergism) for a wide range of organisms. In contrast, admixtures with (a):(b) ratios below 100:1, e.g. 10:1 to 50:1, are much less synergistic, and are active with only a narrower range of organisms. 
     (3) Preservative activity for use compositions is achieved most effectively with an admixture wt. ratio of 100:1 to 2000:1 at use levels of 0.05 to 2% by weight of the finished product. In this amount, the iodopropynyl compound is present in an amount of only 0.5-10 ppm, which significantly reduces the cost and toxicity of the use composition. The methylol compound also is present in an amount of at least 250 ppm. 
     The experimental results upon which these discoveries are based are described below. In these examples, the (a) methylol compound may be selected from diazolidinyl urea (GERMALL® II) N-[1,3-bis(hydroxymethyl)-2,5-dioxo-4-imidazolidinyl]N,N&#39;-bis(hydroxymethyl) urea imidurea (GERMALL® 115), 1,3-dimethylol-5,5-dimethyl hydantoin (DMDMH), sodium hydroxymethylglycinate (SUTTOCIDE® A), glycine anhydride dimethylol (GADM), dimethylhydroxymethyl pyrazole, (1-(3-chloroallyl)-3,5,7-triaza-1-azoniaadamantane chloride (a methylol equivalent), 1,3,5-(trishydroxyethyl)hexahydrotriazine, or hydroxymethyl pyrrolidone; and the (b) iodopropynyl compound is iodopropynyl alcohol (IPGA) or 3-iodo-2-propynylbutyl carbamate (IPBC). 
     1. WATER SOLUBILITY 
     The water solubility or insolubility of admixtures of several methylol compounds with IPBC as 1% aqueous solutions is shown in Tables A through C below. 
     
                       TABLE A______________________________________Weight Ratio of          AmountGermall ® II:IPBC          in ppm        Solubility______________________________________2000:1          5            Soluble1000:1          10           Soluble500:1           20           Soluble200:1           50           Soluble100:1          100           Soluble 50:1          200           Insoluble 20:1          500           Insoluble______________________________________ 
    
     
                       TABLE B______________________________________Weight Ratio of         AmountGADM:IPBC     in ppm         Solubility______________________________________2000:1         5             Soluble1000:1         10            Soluble500:1          20            Soluble200:1          50            Soluble100:1         100            Soluble 50:1         200            Insoluble 20:1         500            Insoluble______________________________________ 
    
     
                       TABLE C______________________________________Weight Ratio of         AmountDMDMH:IPBC    in ppm         Solubility______________________________________2000:1         5             Soluble1000:1         10            Soluble500:1          20            Soluble200:1          50            Soluble100:1         100            Soluble 50:1         200            Insoluble 20:1         500            Insoluble______________________________________ 
    
     These results demonstrate that admixtures having a ratio of 50:1 or 20:1 are insoluble in water whereas at ratios of 100:1 to 2000:1 the admixtures are soluble in water. 
     2. SYNERGISM 
     Tables 1 through 14 below demonstrate the very effective synergistic interaction between compounds &#34;a&#34; and &#34;b&#34; against a broad range of fungi and both gram-negative and gram-positive bacteria. The following organisms were tested: 
     
         ______________________________________        ATCCOrganism     Number   Inoculum Concentration______________________________________Ps. aeruginosa (PSA)*         9027    2.1 × 10.sup.6                          CFU/gm of ProductE. coli (ECOLI)*         8739    4.7 × 10.sup.5                          CFU/gm of ProductStaph. aureus (SA)**         6538    1.6 × 10.sup.6                          CFU/gm of ProductPs. cepacia (PC)*        25416    1.6 × 10.sup.6                          CFU/gm of ProductC. albicans (CAN)***        10231    8.0 × 10.sup.4                          CFU/gm of ProductA. niger (AN)***        16404    2.7 × 10.sup.5                          CFU/gm of Product______________________________________ *gram-negative bacteria **gram-positive bacteria ***fungi 
    
     Table D below lists the static (MIC) and cidal activities of several antimicrobial agents in ppm. These activities are used to calculate the Synergism Index (SI) of admixtures of the present invention. 
     
                                           TABLE D__________________________________________________________________________Static (MIC) and Cidal Activities of Several Antimicrobial Compounds(Static/Cidal Concentrations in ppm)Organism(ATCC #) IPBC  Germall ® II                 GADM   DMDMH IPGA__________________________________________________________________________(SA) (6538)    100/200          400/1600                 400/800                        450/1600                               300/5000(ECOLI) (8739)    50/100          400/1600                 400/800                        400/800                              150/600(PSA) (9027)    800/1200          400/1600                 400/400                        600/1600                              70/70(PC) (25416)    1200/1800          200/400                 200/400                        600/1600                               70/300(CAN) (10231)    50/100          1500/15000                  7500/15000                        8000/16000                               50/300(AN) (16404)    50/100          3200/3200                 1600/3200                        8000/16000                              30/30__________________________________________________________________________ 
    
     The Synergism Index was determined by the same mathematical treatment of such data described by Kull et al. in Applied Microbiology 9, 538-541 (1961) using the following relationship: ##EQU1## where: 1. Q a  = The quantity of Compound a acting alone, producing an endpoint. 
     2. Q b  = The quantity of Compound b acting alone, producing and endpoint. 
     3. Q A  = The quantity of Compound A in mixture, producing an endpoint. 
     4. Q B  = The quantity of Compound B in mixture, producing an endpoint. 
     When SI is equal to 1, a mere additive effect of the components in the mixture is indicated; when SI is less than 1, synergism has occurred; and when SI is greater than 1 it indicates antagonism of the two components. 
     According to this well known method of measuring synergism, the quantity of each component in the various mixtures is compared with the quantity of pure component that is required to reach the same endpoint or to produce the same microbiological effect as the mixture. 
     
                       TABLE 1______________________________________2000:1 Wt. Ratio GERMALL ® II/IPBCUse Level   Organism Q.sub.a Q.sub.b                          Q.sub.A                                Q.sub.B                                      SI______________________________________0.01%   SA       200     1600  0.05  99.95 0.06&#34;       ECOLI    100     1600  0.05  99.95 0.06&#34;       PSA      1200    1600  0.05  99.95 0.06&#34;       PC       1800    1250  0.05  99.95 0.08&#34;       CAN      100     15000 0.05  99.95 0.01&#34;       AN       100     3200  0.05  99.95 0.03 0.025% SA       200     1600  0.125 249.9 0.16&#34;       ECOLI    100     1600  0.125 249.9 0.16&#34;       PSA      1200    1600  0.125 249.9 0.16&#34;       PC       1800    1250  0.125 249.9 0.20&#34;       CAN      100     15000 0.125 249.9 0.02&#34;       AN       100     3200  0.125 249.9 0.080.05%   SA       200     1600  0.25  499.8 0.31&#34;       ECOLI    100     1600  0.25  499.8 0.31&#34;       PSA      1200    1600  0.25  499.8 0.31&#34;       PC       1800    1250  0.25  499.8 0.40&#34;       CAN      100     15000 0.25  499.8 0.04&#34;       AN       100     3200  0.25  499.8 0.160.10%   SA       200     1600  0.5   999.5 0.63&#34;       ECOLI    100     1600  0.5   999.5 0.63&#34;       PSA      1200    1600  0.5   999.5 0.63&#34;       PC       1800    1250  0.5   999.5 0.80&#34;       CAN      100     15000 0.5   999.5 0.07&#34;       AN       100     3200  0.5   999.5 0.320.20%   SA       200     1600  1     1999  1.25&#34;       ECOLI    100     1600  1     1999  1.26&#34;       PSA      1200    1600  1     1999  1.25&#34;       PC       1800    1250  1     1999  1.60&#34;       CAN      100     15000 1     1999  0.14&#34;       AN       100     3200  1     1999  0.630.40%   SA       200     1600  2     3998  2.51&#34;       ECOLI    100     1600  2     3998  2.52&#34;       PSA      1200    1600  2     3998  2.50&#34;       PC       1800    1250  2     3998  3.20&#34;       CAN      100     15000 2     3998  0.29&#34;       AN       100     3200  2     3998  1.270.50%   SA       200     1600  2.5   4997.5                                      3.14&#34;       ECOLI    100     1600  2.5   4997.5                                      3.15&#34;       PSA      1200    1600  2.5   4997.5                                      3.13&#34;       PC       1800    1250  2.5   4997.5                                      4.00&#34;       CAN      100     15000 2.5   4997.5                                      0.36&#34;       AN       100     3200  2.5   4997.5                                      1.59______________________________________ 
    
     
                       TABLE 2______________________________________1000:1 Wt. Ratio GERMALL ® II/IPBCUse Level   Organism Q.sub.a Q.sub.b                          Q.sub.A                                Q.sub.B                                       SI______________________________________0.01%   SA       200     1600  0.1   99.9   0.06&#34;       ECOLI    100     1600  0.1   99.9   0.06&#34;       PSA      1200    1600  0.1   99.9   0.06&#34;       PC       1800    1250  0.1   99.9   0.08&#34;       CAN      100     15000 0.1   99.9   0.01&#34;       AN       100     3200  0.1   99.9   0.03 0.025% SA       200     1600  0.25  249.8  0.16&#34;       ECOLI    100     1600  0.25  249.8  0.16&#34;       PSA      1200    1600  0.25  249.8  0.16&#34;       PC       1800    1250  0.25  249.8  0.20&#34;       CAN      100     15000 0.25  249.8  0.02&#34;       AN       100     3200  0.25  249.8  0.080.05%   SA       200     1600  0.5   499.5  0.31&#34;       ECOLI    100     1600  0.5   499.5  0.32&#34;       PSA      1200    1600  0.5   499.5  0.31&#34;       PC       1800    1250  0.5   499.5  0.40&#34;       CAN      100     15000 0.5   499.5  0.04&#34;       AN       100     3200  0.5   499.5  0.160.10%   SA       200     1600  1     999    0.63&#34;       ECOLI    100     1600  1     999    0.63&#34;       PSA      1200    1600  1     999    0.63&#34;       PC       1800    1250  1     999    0.80&#34;       CAN      100     15000 1     999    0.08&#34;       AN       100     3200  1     999    0.320.20%   SA       200     1600  2     1998   1.26&#34;       ECOLI    100     1600  2     1998   1.27&#34;       PSA      1200    1600  2     1998   1.25&#34;       PC       1800    1250  2     1998   1.60&#34;       CAN      100     15000 2     1998   0.15&#34;       AN       100     3200  2     1998   0.640.40%   SA       200     1600  4     3996   2.52&#34;       ECOLI    100     1600  4     3996   2.54&#34;       PSA      1200    1600  4     3996   2.50&#34;       PC       1800    1250  4     3996   3.20&#34;       CAN      100     15000 4     3996   0.31&#34;       AN       100     3200  4     3996   1.290.50%   SA       200     1600  5     4995   3.15&#34;       ECOLI    100     1600  5     4995   3.17&#34;       PSA      1200    1600  5     4995   3.13&#34;       PC       1800    1250  5     4995   4.00&#34;       CAN      100     15000 5     4995   0.38&#34;       AN       100     3200  5     4995   1.61______________________________________ 
    
     
                       TABLE 3______________________________________500:1 Wt. Ratio GERMALL ® II/IPBCUse Level   Organism Q.sub.a Q.sub.b                          Q.sub.A                                Q.sub.B                                       SI______________________________________0.01%   SA       200     1600  0.2   99.8   0.06&#34;       ECOLI    100     1600  0.2   99.8   0.06&#34;       PSA      1200    1600  0.2   99.8   0.06&#34;       PC       1800    1250  0.2   99.8   0.08&#34;       CAN      100     15000 0.2   99.8   0.01&#34;       AN       100     3200  0.2   99.8   0.03 0.025% SA       200     1600  0.5   249.5  0.16&#34;       ECOLI    100     1600  0.5   249.5  0.16&#34;       PSA      1200    1600  0.5   249.5  0.16&#34;       PC       1800    1250  0.5   249.5  0.20&#34;       CAN      100     15000 0.5   249.5  0.02&#34;       AN       100     3200  0.5   249.5  0.080.05%   SA       200     1600  1     499    0.32&#34;       ECOLI    100     1600  1     499    0.32&#34;       PSA      1200    1600  1     499    0.31&#34;       PC       1800    1250  1     499    0.40&#34;       CAN      100     15000 1     499    0.04&#34;       AN       100     3200  1     499    0.170.10%   SA       200     1600  2     998    0.63&#34;       ECOLI    100     1600  2     998    0.64&#34;       PSA      1200    1600  2     998    0.63&#34;       PC       1800    1250  2     998    0.80&#34;       CAN      100     15000 2     998    0.09&#34;       AN       100     3200  2     998    0.330.20%   SA       200     1600  4     1996   1.27&#34;       ECOLI    100     1600  4     1996   1.29&#34;       PSA      1200    1600  4     1996   1.25&#34;       PC       1800    1250  4     1996   1.60&#34;       CAN      100     15000 4     1996   0.17&#34;       AN       100     3200  4     1996   0.660.40%   SA       200     1600  8     3992   2.54&#34;       ECOLI    100     1600  8     3992   2.58&#34;       PSA      1200    1600  8     3992   2.50&#34;       PC       1800    1250  8     3992   3.20&#34;       CAN      100     15000 8     3992   0.35&#34;       AN       100     3200  8     3992   1.330.50%   SA       200     1600  10    4990   3.17&#34;       ECOLI    100     1600  10    4990   3.22&#34;       PSA      1200    1600  10    4990   3.13&#34;       PC       1800    1250  10    4990   4.00&#34;       CAN      100     15000 10    4990   0.43&#34;       AN       100     3200  10    4990   1.66______________________________________ 
    
     
                       TABLE 4______________________________________200:1 Wt. Ratio GERMALL ® II/IPBCUse Level   Organism Q.sub.a Q.sub.b                          Q.sub.A                                Q.sub.B                                       SI______________________________________0.01%   SA       200     1600  0.5   99.5   0.06&#34;       ECOLI    100     1600  0.5   99.5   0.07&#34;       PSA      1200    1600  0.5   99.5   0.06&#34;       PC       1800    1250  0.5   99.5   0.08&#34;       CAN      100     15000 0.5   99.5   0.01&#34;       AN       100     3200  0.5   99.5   0.04 0.025% SA       200     1600  1.25  248.75 0.16&#34;       ECOLI    100     1600  1.25  248.75 0.17&#34;       PSA      1200    1600  1.25  248.75 0.16&#34;       PC       1800    1250  1.25  248.75 0.20&#34;       CAN      100     15000 1.25  248.75 0.03&#34;       AN       100     3200  1.25  248.75 0.090.05%   SA       200     1600  2.5   497.5  0.32&#34;       ECOLI    100     1600  2.5   497.5  0.34&#34;       PSA      1200    1600  2.5   497.5  0.31&#34;       PC       1800    1250  2.5   497.5  0.40&#34;       CAN      100     15000 2.5   497.5  0.06&#34;       AN       100     3200  2.5   497.5  0.180.10%   SA       200     1600  5     995    0.65&#34;       ECOLI    100     1600  5     995    0.67&#34;       PSA      1200    1600  5     995    0.63&#34;       PC       1800    1250  5     995    0.80&#34;       CAN      100     15000 5     995    0.12&#34;       AN       100     3200  5     995    0.360.20%   SA       200     1600  10    1990   1.29&#34;       ECOLI    100     1600  10    1990   1.34&#34;       PSA      1200    1600  10    1990   1.25&#34;       PC       1800    1250  10    1990   1.60&#34;       CAN      100     15000 10    1990   0.23&#34;       AN       100     3200  10    1990   0.720.40%   SA       200     1600  20    3980   2.59&#34;       ECOLI    100     1600  20    3980   2.69&#34;       PSA      1200    1600  20    3980   2.50&#34;       PC       1800    1250  20    3980   3.20&#34;       CAN      100     15000 20    3980   0.47&#34;       AN       100     3200  20    3980   1.440.50%   SA       200     1600  25    4975   3.23&#34;       ECOLI    100     1600  25    4975   3.36&#34;       PSA      1200    1600  25    4975   3.13&#34;       PC       1800    1250  25    4975   3.99&#34;       CAN      100     15000 25    4975   0.58&#34;       AN       100     3200  25    4975   1.80______________________________________ 
    
     
                       TABLE 5______________________________________100:1 Wt. Ratio GERMALL ® II/IPBCUse Level   Organism Q.sub.a  Q.sub.b                           Q.sub.A                                 Q.sub.B                                      SI______________________________________0.01%   SA       200      1600  1      99  0.07&#34;       ECOLI    100      1600  1      99  0.07&#34;       PSA      1200     1600  1      99  0.06&#34;       PC       1800     1250  1      99  0.08&#34;       CAN      100      15000 1      99  0.02&#34;       AN       100      3200  1      99  0.04 0.025% SA       200      1600  2.5    248 0.17&#34;       ECOLI    100      1600  2.5    248 0.18&#34;       PSA      1200     1600  2.5    248 0.16&#34;       PC       1800     1250  2.5    248 0.20&#34;       CAN      100      15000 2.5    248 0.04&#34;       AN       100      3200  2.5    248 0.100.05%   SA       200      1600  5      495 0.33&#34;       ECOLI    100      1600  5      495 0.36&#34;       PSA      1200     1600  5      495 0.31&#34;       PC       1800     1250  5      495 0.40&#34;       CAN      100      15000 5      495 0.08&#34;       AN       100      3200  5      495 0.200.10%   SA       200      1600  10     990 0.67&#34;       ECOLI    100      1600  10     990 0.72&#34;       PSA      1200     1600  10     990 0.63&#34;       PC       1800     1250  10     990 0.80&#34;       CAN      100      15000 10     990 0.17&#34;       AN       100      3200  10     990 0.410.20%   SA       200      1600  20    1980 1.34&#34;       ECOLI    100      1600  20    1980 1.44&#34;       PSA      1200     1600  20    1980 1.25&#34;       PC       1800     1250  20    1980 1.60&#34;       CAN      100      15000 20    1980 0.33&#34;       AN       100      3200  20    1980 0.820.40%   SA       200      1600  40    3960 2.68&#34;       ECOLI    100      1600  40    3960 2.88&#34;       PSA      1200     1600  40    3960 2.51&#34;       PC       1800     1250  40    3960 3.19&#34;       CAN      100      15000 40    3960 0.66&#34;       AN       100      3200  40    3960 1.640.50%   SA       200      1600  50    4950 3.34&#34;       ECOLI    100      1600  50    4950 3.59&#34;       PSA      1200     1600  50    4950 3.14&#34;       PC       1800     1250  50    4950 3.99&#34;       CAN      100      15000 50    4950 0.83&#34;       AN       100      3200  50    4950 2.05______________________________________ 
    
     
                       TABLE 6______________________________________50:1 Wt. Ratio GERMALL ® II/IPBCUse Level   Organism Q.sub.a  Q.sub.b                           Q.sub.A                                 Q.sub.B                                      SI______________________________________0.01%   SA       200      1600   2     98  0.07&#34;       ECOLI    100      1600   2     98  0.08&#34;       PSA      1200     1600   2     98  0.06&#34;       PC       1800     1250   2     98  0.08&#34;       CAN      100      15000  2     98  0.03&#34;       AN       100      3200   2     98  0.05 0.025% SA       200      1600   5     245 0.18&#34;       ECOLI    100      1600   5     245 0.20&#34;       PSA      1200     1600   5     245 0.16&#34;       PC       1800     1250   5     245 0.20&#34;       CAN      100      15000  5     245 0.07&#34;       AN       100      3200   5     245 0.130.05%   SA       200      1600  10     490 0.36&#34;       ECOLI    100      1600  10     490 0.41&#34;       PSA      1200     1600  10     490 0.31&#34;       PC       1800     1250  10     490 0.40&#34;       CAN      100      15000 10     490 0.13&#34;       AN       100      3200  10     490 0.250.10%   SA       200      1600  20     980 0.71&#34;       ECOLI    100      1600  20     980 0.81&#34;       PSA      1200     1600  20     980 0.63&#34;       PC       1800     1250  20     980 0.80&#34;       CAN      100      15000 20     980 0.27&#34;       AN       100      3200  20     980 0.510.20%   SA       200      1600  40    1960 1.43&#34;       ECOLI    100      1600  40    1960 1.63&#34;       PSA      1200     1600  40    1960 1.26&#34;       PC       1800     1250  40    1960 1.59&#34;       CAN      100      15000 40    1960 0.53&#34;       AN       100      3200  40    1960 1.010.40%   SA       200      1600  80    3920 2.85&#34;       ECOLI    100      1600  80    3920 3.25&#34;       PSA      1200     1600  80    3920 2.52&#34;       PC       1800     1250  80    3920 3.18&#34;       CAN      100      15000 80    3920 1.06&#34;       AN       100      3200  80    3920 2.030.50%   SA       200      1600  100   4900 3.56&#34;       ECOLI    100      1600  100   4900 4.06&#34;       PSA      1200     1600  100   4900 3.15&#34;       PC       1800     1250  100   4990 3.98&#34;       CAN      100      15000 100   4900 1.33&#34;       AN       100      3200  100   4900 2.53______________________________________ 
    
     
                       TABLE 7______________________________________20:1 Wt. Ratio GERMALL ® II/IPBCUse Level   Organism Q.sub.a Q.sub.b                          Q.sub.A                                Q.sub.B                                       SI______________________________________0.01%   SA       200     1600  5     95     0.08&#34;       ECOLI    100     1600  5     95     0.11&#34;       PSA      1200    1600  5     95     0.06&#34;       PC       1800    1250  5     95     0.08&#34;       CAN      100     15000 5     95     0.06&#34;       AN       100     3200  5     95     0.08 0.025% SA       200     1600  12.5  237.5  0.21&#34;       ECOLI    100     1600  12.5  237.5  0.27&#34;       PSA      1200    1600  12.5  237.5  0.16&#34;       PC       1800    1250  12.5  237.5  0.20&#34;       CAN      100     15000 12.5  237.5  0.14&#34;       AN       100     3200  12.5  237.5  0.200.05%   SA       200     1600  25    475    0.42&#34;       ECOLI    100     1600  25    475    0.55&#34;       PSA      1200    1600  25    475    0.32&#34;       PC       1800    1250  25    475    0.39&#34;       CAN      100     15000 25    475    0.28&#34;       AN       100     3200  25    475    0.400.10%   SA       200     1600  50    950    0.84&#34;       ECOLI    100     1600  50    950    1.09&#34;       PSA      1200    1600  50    950    0.64&#34;       PC       1800    1250  50    950    0.79&#34;       CAN      100     15000 50    950    0.56&#34;       AN       100     3200  50    950    0.800.20%   SA       200     1600  100   1900   1.69&#34;       ECOLI    100     1600  100   1900   2.19&#34;       PSA      1200    1600  100   1900   1.27&#34;       PC       1800    1250  100   1900   1.58&#34;       CAN      100     15000 100   1900   1.13&#34;       AN       100     3200  100   1900   1.590.40%   SA       200     1600  200   4800   4.00&#34;       ECOLI    100     1600  200   4800   5.00&#34;       PSA      1200    1600  200   4800   3.17&#34;       PC       1800    1250  200   4800   3.95&#34;       CAN      100     15000 200   4800   2.32&#34;       AN       100     3200  200   4800   3.500.50%   SA       200     1600  250   4750   4.22&#34;       ECOLI    100     1600  250   4750   5.47&#34;       PSA      1200    1600  250   4750   3.18&#34;       PC       1800    1250  250   4750   3.94&#34;       CAN      100     15000 250   4750   2.82&#34;       AN       100     3200  250   4750   3.98______________________________________ 
    
     
                       TABLE 8______________________________________2000:1 Wt. Ratio DMDMH/IPBCUse Level   Organism Q.sub.a Q.sub.b                          Q.sub.A                                Q.sub.B                                       SI______________________________________0.01%   SA       200     1600  0.05  99.95  0.06&#34;       ECOLI    100      800  0.05  99.95  0.13&#34;       PSA      1200    1600  0.05  99.95  0.06&#34;       PC       1800    1600  0.05  99.95  0.06&#34;       CAN      100     16000 0.05  99.95  0.01&#34;       AN       100     16000 0.05  99.95  0.01 0.025% SA       200     1600  0.125 249.88 0.16&#34;       ECOLI    100      800  0.125 249.88 0.31&#34;       PSA      1200    1600  0.125 249.88 0.16&#34;       PC       1800    1600  0.125 249.88 0.16&#34;       CAN      100     16000 0.125 249.88 0.02&#34;       AN       100     16000 0.125 249.88 0.020.05%   SA       200     1600  0.25  499.75 0.31&#34;       ECOLI    100      800  0.25  499.75 0.63&#34;       PSA      1200    1600  0.25  499.75 0.31&#34;       PC       1800    1600  0.25  499.75 0.31&#34;       CAN      100     16000 0.25  499.75 0.03&#34;       AN       100     16000 0.25  499.75 0.030.10%   SA       200     1600  0.5   999.5  0.63&#34;       ECOLI    100      800  0.5   999.5  1.25&#34;       PSA      1200    1600  0.5   999.5  0.63&#34;       PC       1800    1600  0.5   999.5  0.62&#34;       CAN      100     16000 0.5   999.5  0.07&#34;       AN       100     16000 0.5   999.5  0.070.20%   SA       200     1600  1     1999   1.25&#34;       ECOLI    100      800  1     1999   2.51&#34;       PSA      1200    1600  1     1999   1.25&#34;       PC       1800    1600  1     1999   1.25&#34;       CAN      100     16000 1     1999   0.13&#34;       AN       100     16000 1     1999   0.130.40%   SA       200     1600  2     3998   2.51&#34;       ECOLI    100      800  2     3998   5.02&#34;       PSA      1200    1600  2     3998   2.50&#34;       PC       1800    1600  2     3998   2.50&#34;       CAN      100     16000 2     3998   0.27&#34;       AN       100     16000 2     3998   0.270.50%   SA       200     1600  2.5   4997.5 3.14&#34;       ECOLI    100      800  2.5   4997.5 6.27&#34;       PSA      1200    1600  2.5   4997.5 3.13&#34;       PC       1800    1600  2.5   4997.5 3.12&#34;       CAN      100     16000 2.5   4997.5 0.34&#34;       AN       100     16000 2.5   4997.5 0.34______________________________________ 
    
     
                       TABLE 9______________________________________1000:1 Wt. Ratio DMDMH/IPBCUse Level   Organism Q.sub.a Q.sub.b                          Q.sub.A                                Q.sub.B                                       SI______________________________________0.01.%  SA       200     1600  0.1   99.9   0.06&#34;       ECOLI    100      800  0.1   99.9   0.13&#34;       PSA      1200    1600  0.1   99.9   0.06&#34;       PC       1800    1600  0.1   99.9   0.06&#34;       CAN      100     16000 0.1   99.9   0.01&#34;       AN       100     16000 0.1   99.9   0.01 0.025% SA       200     1600  0.25  249.8  0.16&#34;       ECOLI    100      800  0.25  249.8  0.31&#34;       PSA      1200    1600  0.25  249.8  0.16&#34;       PC       1800    1600  0.25  249.8  0.16&#34;       CAN      100     16000 0.25  249.8  0.02&#34;       AN       100     16000 0.25  249.8  0.020.05%   SA       200     1600  0.5   499.5  0.31&#34;       ECOLI    100      800  0.5   499.5  0.63&#34;       PSA      1200    1600  0.5   499.5  0.31&#34;       PC       1800    1600  0.5   499.5  0.31&#34;       CAN      100     16000 0.5   499.5  0.04&#34;       AN       100     16000 0.5   499.5  0.040.10%   SA       200     1600  1     999    0.63&#34;       ECOLI    100      800  1     999    1.26&#34;       PSA      1200    1600  1     999    0.63&#34;       PC       1800    1600  1     999    0.62&#34;       CAN      100     16000 1     999    0.07&#34;       AN       100     16000 1     999    0.070.20%   SA       200     1600  2     1998   1.26&#34;       ECOLI    100      800  2     1998   2.52&#34;       PSA      1200    1600  2     1998   1.25&#34;       PC       1800    1600  2     1998   1.25&#34;       CAN      100     16000 2     1998   0.14&#34;       AN       100     16000 2     1998   0.140.40%   SA       200     1600  4     3996   2.52&#34;       ECOLI    100      800  4     3996   5.04&#34;       PSA      1200    1600  4     3996   2.50&#34;       PC       1800    1600  4     3996   2.50&#34;       CAN      100     16000 4     3996   0.29&#34;       AN       100     16000 4     3996   0.290.50%   SA       200     1600  5     4995   3.15&#34;       ECOLI    100      800  5     4995   6.29&#34;       PSA      1200    1600  5     4995   3.13&#34;       PC       1800    1600  5     4995   3.12&#34;       CAN      100     16000 5     4995   0.36&#34;       AN       100     16000 2.5   4997.5 0.36______________________________________ 
    
     
                       TABLE 10______________________________________500:1 Wt. Ratio DMDMH/IPBCUse Level   Organism Q.sub.a Q.sub.b                          Q.sub.A                                Q.sub.B                                       SI______________________________________0.01%   SA       200     1600  0.2   99.8   0.06&#34;       ECOLI    100      800  0.2   99.8   0.13&#34;       PSA      1200    1600  0.2   99.8   0.06&#34;       PC       1800    1600  0.2   99.8   0.06&#34;       CAN      100     16000 0.2   99.8   0.01&#34;       AN       100     16000 0.2   99.8   0.010.25%   SA       200     1600  0.5   249.5  0.16&#34;       ECOLI    100      800  0.5   249.5  0.32&#34;       PSA      1200    1600  0.5   249.5  0.16&#34;       PC       1800    1600  0.5   249.5  0.16&#34;       CAN      100     16000 0.5   249.5  0.02&#34;       AN       100     16000 0.5   249.5  0.020.05%   SA       200     1600  1     499    0.32&#34;       ECOLI    100      800  1     499    0.63&#34;       PSA      1200    1600  1     499    0.31&#34;       PC       1800    1600  1     499    0.31&#34;       CAN      100     16000 1     499    0.04&#34;       AN       100     16000 1     499    0.040.10%   SA       200     1600  2     998    0.63&#34;       ECOLI    100      800  2     998    1.27&#34;       PSA      1200    1600  2     998    0.63&#34;       PC       1800    1600  2     998    0.62&#34;       CAN      100     16000 2     998    0.08&#34;       AN       100     16000 2     998    0.080.20%   SA       200     1600  4     1996   1.27&#34;       ECOLI    100      800  4     1996   2.54&#34;       PSA      1200    1600  4     1996   1.25&#34;       PC       1800    1600  4     1996   1.25&#34;       CAN      100     16000 4     1996   0.16&#34;       AN       100     16000 4     1996   0.160.40%   SA       200     1600  8     3992   2.54&#34;       ECOLI    100      800  8     3992   5.07&#34;       PSA      1200    1600  8     3992   2.50&#34;       PC       1800    1600  8     3992   2.50&#34;       CAN      100     16000 8     3992   0.33&#34;       AN       100     16000 8     3992   0.330.50%   SA       200     1600  10    4900   3.11&#34;       ECOLI    100      800  10    4900   6.23&#34;       PSA      1200    1600  10    4900   3.07&#34;       PC       1800    1600  10    4900   3.07&#34;       CAN      100     16000 10    4900   0.41&#34;       AN       100     16000 10    4900   0.41______________________________________ 
    
     
                       TABLE 11______________________________________200:1 Wt. Ratio DMDMH/IPBCUse Level   Organism Q.sub.a Q.sub.b                          Q.sub.A                                Q.sub.B                                       SI______________________________________0.01%   SA       200     1600  0.5   99.5   0.06&#34;       ECOLI    100      800  0.5   99.5   0.13&#34;       PSA      1200    1600  0.5   99.5   0.06&#34;       PC       1800    1600  0.5   99.5   0.06&#34;       CAN      100     16000 0.5   99.5   0.01&#34;       AN       100     16000 0.5   99.5   0.01 0.025% SA       200     1600  1.25  248.75 0.16&#34;       ECOLI    100      800  1.25  248.75 0.32&#34;       PSA      1200    1600  1.25  248.75 0.16&#34;       PC       1800    1600  1.25  248.75 0.16&#34;       CAN      100     16000 1.25  248.75 0.03&#34;       AN       100     16000 1.25  248.75 0.030.05%   SA       200     1600  2.5   497.5  0.32&#34;       ECOLI    100      800  2.5   497.5  0.65&#34;       PSA      1200    1600  2.5   497.5  0.31&#34;       PC       1800    1600  2.5   497.5  0.31&#34;       CAN      100     16000 2.5   497.5  0.06&#34;       AN       100     16000 2.5   497.5  0.060.10%   SA       200     1600  5     995    0.65&#34;       ECOLI    100      800  5     995    1.29&#34;       PSA      1200    1600  5     995    0.63&#34;       PC       1800    1600  5     995    0.62&#34;       CAN      100     16000 5     995    0.11&#34;       AN       100     16000 5     995    0.110.20%   SA       200     1600  10    1990   1.29&#34;       ECOLI    100      800  10    1990   2.59&#34;       PSA      1200    1600  10    1990   1.25&#34;       PC       1800    1600  10    1990   1.25&#34;       CAN      100     16000 10    1990   0.22&#34;       AN       100     16000 10    1990   0.220.40%   SA       200     1600  20    3980   2.59&#34;       ECOLI    100      800  20    3980   5.18&#34;       PSA      1200    1600  20    3980   2.50&#34;       PC       1800    1600  20    3980   2.50&#34;       CAN      100     16000 20    3980   0.45&#34;       AN       100     16000 20    3980   0.450.50%   SA       200     1600  25    4975   3.23&#34;       ECOLI    100      800  25    4975   6.47&#34;       PSA      1200    1600  25    4975   3.13&#34;       PC       1800    1600  25    4975   3.12&#34;       CAN      100     16000 25    4975   0.56&#34;       AN       100     16000 25    4975   0.56______________________________________ 
    
     
                       TABLE 12______________________________________100:1 Wt. Ratio DMDMH/IPBCUse Level   Organism Q.sub.a Q.sub.b                          Q.sub.A                                Q.sub.B                                      SI______________________________________0.01%   SA       200     1600  1      99   0.07&#34;       ECOLI    100      800  1      99   0.13&#34;       PSA      1200    1600  1      99   0.06&#34;       PC       1800    1600  1      99   0.06&#34;       CAN      100     16000 1      99   0.02&#34;       AN       100     16000 1      99   0.02 0.025% SA       200     1600  2.5    248  0.17&#34;       ECOLI    100      800  2.5    248  0.33&#34;       PSA      1200    1600  2.5    248  0.16&#34;       PC       1800    1600  2.5    248  0.16&#34;       CAN      100     16000 2.5    248  0.04&#34;       AN       100     16000 2.5    248  0.040.05%   SA       200     1600  5      495  0.33&#34;       ECOLI    100      800  5      495  0.67&#34;       PSA      1200    1600  5      495  0.31&#34;       PC       1800    1600  5      495  0.31&#34;       CAN      100     16000 5      495  0.08&#34;       AN       100     16000 5      495  0.080.10%   SA       200     1600  10     990  0.67&#34;       ECOLI    100      800  10     990  1.34&#34;       PSA      1200    1600  10     990  0.63&#34;       PC       1800    1600  10     990  0.62&#34;       CAN      100     16000 10     990  0.16&#34;       AN       100     16000 10     990  0.160.20%   SA       200     1600  20    1980  1.34&#34;       ECOLI    100      800  20    1980  2.68&#34;       PSA      1200    1600  20    1980  1.25&#34;       PC       1800    1600  20    1980  1.25&#34;       CAN      100     16000 20    1980  0.32&#34;       AN       100     16000 20    1980  0.320.40%   SA       200     1600  40    3960  2.68&#34;       ECOLI    100      800  40    3960  5.35&#34;       PSA      1200    1600  40    3960  2.51&#34;       PC       1800    1600  40    3960  2.50&#34;       CAN      100     16000 40    3960  0.65&#34;       AN       100     16000 40    3960  0.650.50%   SA       200     1600  50    4950  3.34&#34;       ECOLI    100      800  50    4950  6.69&#34;       PSA      1200    1600  50    4950  3.14&#34;       PC       1800    1600  50    4950  3.12&#34;       CAN      100     16000 50    4950  0.81&#34;       AN       100     16000 50    4950  0.81______________________________________ 
    
     
                       TABLE 13______________________________________50:1 Wt. Ratio DMDMH/IPBCUse Level   Organism Q.sub.a Q.sub.b                          Q.sub.A                                Q.sub.B                                      SI______________________________________0.01%   SA       200     1600   2     98   0.07&#34;       ECOLI    100      800   2     98   0.14&#34;       PSA      1200    1600   2     98   0.06&#34;       PC       1800    1600   2     98   0.06&#34;       CAN      100     16000  2     98   0.03&#34;       AN       100     16000  2     98   0.03 0.025% SA       200     1600   5     245  0.18&#34;       ECOLI    100      800   5     245  0.36&#34;       PSA      1200    1600   5     245  0.16&#34;       PC       1800    1600   5     245  0.16&#34;       CAN      100     16000  5     245  0.07&#34;       AN       100     16000  5     245  0.070.05%   SA       200     1600  10     490  0.36&#34;       ECOLI    100      800  10     490  0.71&#34;       PSA      1200    1600  10     490  0.31&#34;       PC       1800    1600  10     490  0.31&#34;       CAN      100     16000 10     490  0.13&#34;       AN       100     16000 10     490  0.130.10%   SA       200     1600  20     980  0.71&#34;       ECOLI    100      800  20     980  1.43&#34;       PSA      1200    1600  20     980  0.63&#34;       PC       1800    1600  20     980  0.62&#34;       CAN      100     16000 20     980  0.26&#34;       AN       100     16000 20     980  0.260.20%   SA       200     1600  40    1960  1.43&#34;       ECOLI    100      800  40    1960  2.85&#34;       PSA      1200    1600  40    1960  1.26&#34;       PC       1800    1600  40    1960  1.25&#34;       CAN      100     16000 40    1960  0.52&#34;       AN       100     16000 40    1960  0.520.40%   SA       200     1600  80    3920  2.85&#34;       ECOLI    100      800  80    3920  5.70&#34;       PSA      1200    1600  80    3920  2.52&#34;       PC       1800    1600  80    3920  2.49&#34;       CAN      100     16000 80    3920  1.05&#34;       AN       100     16000 80    3920  1.050.50%   SA       200     1600  100   4900  3.56&#34;       ECOLI    100      800  100   4900  7.13&#34;       PSA      1200    1600  100   4900  3.15&#34;       PC       1800    1600  100   4900  3.12&#34;       CAN      100     16000 100   4900  1.31&#34;       AN       100     16000 100   4900  1.31______________________________________ 
    
     
                       TABLE 14______________________________________20:1 Wt. Ratio DMDMH/IPBCUse Level   Organism Q.sub.a Q.sub.b                          Q.sub.A                                Q.sub.B                                       SI______________________________________0.01%   SA       200     1600  5     95     0.08   ECOLI    100      800  5     95     0.17   PSA      1200    1600  5     95     0.06   PC       1800    1600  5     95     0.06   CAN      100     16000 5     95     0.06   AN       100     16000 5     95     0.06 0.025% SA       200     1600  12.5  237.5  0.21   ECOLI    100      800  12.5  237.5  0.42   PSA      1200    1600  12.5  237.5  0.16   PC       1800    1600  12.5  237.5  0.16   CAN      100     16000 12.5  237.5  0.14   AN       100     16000 12.5  237.5  0.140.05%   SA       200     1600  25    475    0.42   ECOLI    100      800  25    475    0.84   PSA      1200    1600  25    475    0.32   PC       1800    1600  25    475    0.31   CAN      100     16000 25    475    0.28   AN       100     16000 25    475    0.280.10%   SA       200     1600  50    950    0.84   ECOLI    100      800  50    950    1.69   PSA      1200    1600  50    950    0.64   PC       1800    1600  50    950    0.62   CAN      100     16000 50    950    0.56   AN       100     16000 50    950    0.560.20%   SA       200     1600  100   1900   1.69   ECOLI    100      800  100   1900   3.38   PSA      1200    1600  100   1900   1.27   PC       1800    1600  100   1900   1.24   CAN      100     16000 100   1900   1.12   AN       100     16000 100   1900   1.120.40%   SA       200     1600  200   4800   4.00   ECOLI    100      800  200   4800   8.00   PSA      1200    1600  200   4800   3.17   PC       1800    1600  200   4800   3.11   CAN      100     16000 200   4800   2.30   AN       100     16000 200   4800   2.300.50%   SA       200     1600  250   4750   4.22   ECOLI    100      800  250   4750   8.44   PSA      1200    1600  250   4750   3.18   PC       1800    1600  250   4750   3.11   CAN      100     16000 250   4750   2.80   AN       100     16000 250   4750   2.80______________________________________ 
    
     Similar SI results also were found with GADM and SUTTOCIDE® A as the methylol compound in place of Germall® II or DMDMH in admixtures with IPBC over the same wt. ratios and use level ranges as shown in the Tables 1-14 above. 
     Tables 1 through 14 above illustrate the synergism of IPBC (compound B) with Germall® II or DMDMH (compound A) at weight ratios of A:B of 2000:1, 1000:1, 500:1, 200:1, 100:1, 50:1 and 20:1. Synergism is very effective for all ratios at low use levels, e.g. 0.01% to 0.1%, against all tested gram-positive, gram-negative organisms and fungi organisms. At slightly higher use concentrations, e.g. 0.20 to 0.50%, all tested ratios were synergistic against Candida albicans and A. niger also. However, at 50:1 and 20:1 ratios, the synergistic effect is negligible at the 0.01 to 0.1% use levels, and non-synergistic even against Candida albicans and A. niger at use levels of 0.20 to 0.50%. 
     The SI values were lower for Germall® II as the methylol compound in the admixtures as compared to DMDMH. 
     Similar results were obtained when iodopropynyl alcohol (IPGA) was substituted for IPBC in the admixtures described above. 
     3. PRESERVATIVE ACTIVITY (CHALLENGE TEST) 
     A typical cosmetic emulsion was prepared for microbiological challenge testing and predetermined admixtures of a methylol compound and IPBC were added at various use levels. The emulsion thus prepared had the following composition: 
     
         ______________________________________               % wt.______________________________________Phase AStearic Acid          5.00Mineral Oil           2.50Cetyl Alcohol         1.00Lareth-5 and Ceteth-5 and                 0.50Oleth-5 and Steareth-5Glycerol Monostearate and                 1.50Polyoxyethylene StearatePhase BDeionized Water       88.0Triethanolamine 99%   1.00Citric Acid 30% aqueous solution                 0.60Preservative Admixture                 qs______________________________________ 
    
     To prepare the emulsion, Phases A and B were heated separately to 75°-80° C. Phase A then was added to Phase B with mixing. The mixture then was cooled to 55°-60° C. At this point the desired amount of the preservative admixture was added and the product was cooled to 50° C. while stirring. The citric acid solution then was added to adjust the pH and the mixture was stirred until a temperature of 30° C. was reached. 
     The challenge tests were carried out using the following microorganisms: SA, ECOLI, PSA, PC, AN and CAN, in this manner. 50 g. aliquots of the test emulsion containing various amounts of the preservative admixture were inoculated with approximately 107-108 of the challenge organisms. The test samples then were stirred to disperse the challenge inoculum. The samples were incubated and assayed at 48 hours, 7, 14, 21 and 28 days. The assays were performed on 1 g. of the test sample by serially diluting 10 1  to 10 6  of the original concentration. The plating medium for bacteria was Letheen agar and for fungi it was low pH Mycophil agar with Tween 20. Each plated sample was incubated for 48 hours at 37° C. for bacteria, 5 days at 25° C. for mold, and 3 days at 25° C. for fungi. After incubation, readings of the number of colonies per milliliter (cfu/ml) were made. At 21 days the test product was reinoculated with half of the original inoculum. The data is presented in Tables 15-23 below. 
     
                       TABLE 15______________________________________2000:1 GERMALL ® II/IPBC______________________________________Test           48            14Level Organism Hours   7 Days                        Days  21 Days                                     28 Days______________________________________0.01% AN       69,000  260,000                        190,000                              17,000 4,500&#34;     CAN      98,000  76,000                        1,400 3,100  19,000&#34;     ECOLI    110,000 290,000                        2,400 138,000                                     560,000&#34;     PC       &lt;10     &lt;10   &lt;10   &lt;10    &lt;10&#34;     PSA      &lt;10     &lt;10   &lt;10   &lt;10    &lt;10&#34;     SA       190,000 220   &lt;10   &lt;10    3,7000.025% AN       2,800   10    10    &lt;10    220&#34;     CAN      58,000  29,000                        18,000                              56,000 110,000&#34;     ECOLI    39,000  10    &lt;10   &lt;10    &lt;10&#34;     PC       &lt;10     &lt;10   &lt;10   &lt;10    &lt;10&#34;     PSA      &lt;10     &lt;10   &lt;10   &lt;10    &lt;10&#34;     SA       37,000  170   &lt;10   &lt;10    &lt;100.05% AN       20      &lt;10   &lt;10   &lt;10    &lt;10&#34;     CAN      19,000  6,600 70    &lt;10    320&#34;     ECOLI    3,400   &lt;10   &lt;10   &lt;10    &lt;10&#34;     PC       &lt;10     &lt;10   &lt;10   &lt; 10   &lt;10&#34;     PSA      &lt;10     &lt;10   &lt;10   &lt;10    &lt;10&#34;     SA       31,000  &lt;10   &lt;10   &lt;10    &lt;100.1%  AN       &lt;10     &lt;10   &lt;10   &lt;10    &lt;10&#34;     CAN      180     &lt;10   &lt;10   &lt;10    &lt;10&#34;     ECOLI    &lt;10     &lt;10   &lt;10   &lt;10    &lt;10&#34;     PC       &lt;10     &lt;10   &lt;10   &lt;10    &lt;10&#34;     PSA      &lt;10     &lt;10   &lt;10   &lt;10    &lt;10&#34;     SA       750     &lt;10   &lt;10   &lt;10    &lt;100.2%  AN       &lt;10     &lt;10   &lt;10   &lt;10    &lt;10&#34;     CAN      &lt;10     &lt;10   &lt;10   &lt;10    &lt;10&#34;     ECOLI    &lt;10     &lt;10   &lt;10   &lt;10    &lt;10&#34;     PC       &lt;10     &lt;10   &lt;10   &lt;10    &lt;10&#34;     PSA      &lt;10     &lt;10   &lt;10   &lt;10    &lt;10&#34;     SA       &lt;10     &lt;10   &lt;10   &lt;10    &lt;10______________________________________Unpreserved controlOrganism  48 Hours 7 Days   14 Days                           21 Days  28 Days______________________________________AN     52,000   27,000   19,000 19,000  19,000CAN    110,000  130,000  240,000                           180,000 240,000ECOLI  54,000   140,000  170,000                           170,000 74,000PC     6,400,000           6,400,000                    2,000,000                           6,700,000                                   29,000PSA    110,000  700      110,000                           290,000 85,000SA     2,800,000           250,000  51,000 3,700   330______________________________________           Inoculum Concentration           Organism                  0 Hours  21 Days______________________________________           AN     26,000   53,000           CAN    1,000,000                           1,900,000           ECOLI  3,600,000                           170,000           PC     3,400,000                           87,000           PSA    4,500,000                           390,000           SA     4,100,000                           200,000______________________________________ 
    
     
                       TABLE 16______________________________________1000:1 GERMALL ® II/IPBC______________________________________Test           48            14    21Level Organism Hours   7 Days                        Days  Days  28 Days______________________________________0.01% AN       34,000  3,500 80    10    &lt;10&#34;     CAN      420,000 24,000                        950   6,400 6,400,000&#34;     ECOLI    120,000 63,000                        93,000                              92,000                                    9,600,000&#34;     PC       10      25,000                        1,600 15,800                                    33,000,000&#34;     PSA      &lt;10     &lt;10   &lt;10   &lt;10   &lt;10&#34;     SA       100,000 1,400 &lt;10   &lt;10   5,0000.025% AN       530     10    &lt;10   &lt;10   &lt;10&#34;     CAN      34,000  750   10    770   240,000&#34;     ECOLI    120,000 &lt;10   &lt;10   &lt;10   &lt;10&#34;     PC       &lt;10     &lt;10   &lt;10   &lt;10   &lt;10&#34;     PSA      &lt;10     &lt;10   &lt;10   &lt;10   &lt;10&#34;     SA       37,000  170   &lt;10   &lt;10   &lt;100.05% AN       &lt;10     &lt;10   &lt;10   &lt;10   &lt;10&#34;     CAN      13,000  &lt;10   &lt;10   &lt;10   &lt;10&#34;     ECOLI    68,000  &lt;10   &lt;10   &lt;10   &lt;10&#34;     PC       &lt;10     &lt;10   &lt; 10  &lt;10   &lt;10&#34;     PSA      &lt;10     &lt;10   &lt;10   &lt;10   &lt;10&#34;     SA       21,000  &lt;10   &lt;10   &lt;10   &lt;100.1%  AN       &lt;10     &lt;10   &lt;10   &lt;10   &lt;10&#34;     CAN      10      &lt;10   &lt;10   &lt;10   &lt;10&#34;     ECOLI    &lt;10     &lt;10   &lt;10   &lt;10   &lt;10&#34;     PC       &lt;10     &lt;10   &lt;10   &lt;10   &lt;10&#34;     PSA      &lt;10     &lt;10   &lt;10   &lt;10   &lt;10&#34;     SA       1,400   &lt;10   &lt;10   &lt;10   &lt;100.2%  AN       &lt;10     &lt;10   &lt;10   &lt;10   &lt;10&#34;     CAN      &lt;10     &lt;10   &lt;10   &lt;10   &lt;10&#34;     ECOLI    &lt;10     &lt;10   &lt;10   &lt;10   &lt;10&#34;     PC       &lt;10     &lt;10   &lt;10   &lt;10   &lt;10&#34;     PSA      &lt;10     &lt;10   &lt;10   &lt;10   &lt;10&#34;     SA       &lt;10     &lt;10   &lt;10   &lt;10   &lt;10______________________________________Unpreserved controlOrganism  48 Hours 7 Days   14 Days                           21 Days 28 Days______________________________________AN     52,000   27,000   19,000 19,000  19,000CAN    110,000  130,000  240,000                           180,000 240,000ECOLI  54,000   140,000  170,000                           170,000 74,000PC     6,400,000           6,400,000                    2,000,000                           6,700,000                                   29,000PSA    110,000  700      110,000                           290,000 85,000SA     2,800,000           250,000  51,000 3,700   330______________________________________           Inoculum Concentration           Organism                  0 Hours  21 Days______________________________________           AN     26,000   53,000           CAN    1,000,000                           1,900,000           ECOLI  3,600,000                           170,000           PC     3,400,000                           87,000           PSA    4,500,000                           390,000           SA     4,100,000                           200,000______________________________________ 
    
     
                                           TABLE 17__________________________________________________________________________500:1 GERMALL ® II/IPBC__________________________________________________________________________Test Level Organism      48 Hours           7 Days               14 Days                    21 Days                         28 Days__________________________________________________________________________0.01% AN   23,000           40  &lt;10  &lt;10  &lt;10&#34;     CAN  170,000           5,600               290  200  &lt;10&#34;     ECOLI      90,000           57,000               95,000                    70,000                         240,000&#34;     PC   10   &lt;10 &lt;10  &gt;10,000                         42,000,000&#34;     PSA  &lt;10  &lt;10 &lt;10  &lt;10  &lt;10&#34;     SA   380,000           440 &lt;10  &lt;10  5,1000.05% AN   &lt;10  &lt;10 &lt;10  &lt;10  &lt;10&#34;     CAN  8,700           &lt;10 &lt;10  &lt;10  &lt;10&#34;     ECOLI      60,000           &lt;10 &lt;10  &lt;10  &lt;10&#34;     PC   &lt;10  &lt;10 &lt;10  &lt;10  &lt;10&#34;     PSA  &lt;10  &lt;10 &lt;10  &lt;10  &lt;10&#34;     SA   31,000           &lt;10 &lt;10  &lt;10  &lt;100.1%  AN   &lt;10  &lt;10 &lt;10  &lt;10  &lt;10&#34;     CAN  &lt;10  &lt;10 &lt;10  &lt;10  &lt;10&#34;     ECOLI      &lt;10  &lt;10 &lt;10  &lt;10  &lt;10&#34;     PC   &lt;10  &lt;10 &lt;10  &lt;10  &lt;10&#34;     PSA  &lt;10  &lt;10 &lt;10  &lt;10  &lt;10&#34;     SA   890  &lt;10 &lt;10  &lt;10  &lt;100.2%  AN   &lt;10  &lt;10 &lt;10  &lt;10  &lt;10&#34;     CAN  &lt;10  &lt;10 &lt;10  &lt;10  &lt;10&#34;     ECOLI      &lt;10  &lt;10 &lt;10  &lt;10  &lt;10&#34;     PC   &lt;10  &lt;10 &lt;10  &lt;10  &lt;10&#34;     PSA  &lt;10  &lt;10 &lt;10  &lt;10  &lt;10&#34;     SA   &lt;10  &lt;10 &lt;10  &lt;10  &lt;10__________________________________________________________________________Unpreserved controlOrganism 48 Hours        7 Days 14 Days                     21 Days                           28 Days__________________________________________________________________________AN    6,100  520,000               18,000                     5,000 11,000CAN   1,000,000        710,000               95,000                     12,000                           64,000ECOLI 7,100,000        6,200,000               610,000                     350,000                           120,000PC    14,600,000        160,000,000               3,600,000                     2,720,000                           9,500,000PSA   20     900    130   4,100 &gt;100,000SA    43,000,000        600,000               1,000 220   &lt;10__________________________________________________________________________                Inoculum Concentration                Organism                     0 Hours                          21 Days__________________________________________________________________________                AN   19,000                          4,700,000                CAN  340,000                          16,000,000                ECOLI                     3,900,000                          1,480,000                PC   3,800,000                          1,380,000                PSA  9,200,000                          730,000                SA   4,800,000                          360,000__________________________________________________________________________ 
    
     
                                           TABLE 18__________________________________________________________________________200:1 GERMALL ® II/IPBC__________________________________________________________________________Test Level Organism      48 Hours           7 Days                14 Days                      21 Days                           28 Days__________________________________________________________________________0.01% AN   47,000           320  10    &lt;10  &lt;10&#34;     CAN  810,000           450,000                410,000                      190,000                           63,000&#34;     ECOLI      220,000           7,600                &lt;10   850  &gt;1,000,000&#34;     PC   10,000           500,000                1,900,000                      1,100,000                           193,000&#34;     PSA  &lt;10  &lt;10  &lt;10   &lt;10  &lt;10&#34;     SA   190,000           23,000                120   &lt;10  &lt;100.05% AN   &lt;10  &lt;10  &lt;10   &lt;10  &lt;10&#34;     CAN  190  &lt;10  &lt;10   &lt;10  &lt;10&#34;     ECOLI      37,000           &lt;10  &lt;10   &lt;10  &lt;10&#34;     PC   &lt;10  &lt;10  &lt;10   &lt;10  &lt;10&#34;     PSA  &lt;10  &lt;10  &lt;10   &lt;10  &lt;10&#34;     SA   19,000           &lt;10  &lt;10   &lt;10  &lt;100.1%  AN   &lt;10  &lt;10  &lt;10   &lt;10  &lt;10&#34;     CAN  10   &lt;10  &lt;10   &lt;10  &lt;10&#34;     ECOLI      &lt;10  &lt;10  &lt;10   &lt;10  &lt;10&#34;     PC   &lt;10  &lt;10  &lt;10   &lt;10  &lt;10&#34;     PSA  &lt;10  &lt;10  &lt;10   &lt;10  &lt;10&#34;     SA   45,000           &lt;10  &lt;10   &lt;10  &lt;100.2%  AN   &lt;10  &lt;10  &lt;10   &lt;10  &lt;10&#34;     CAN  &lt;10  &lt;10  &lt;10   &lt;10  &lt;10&#34;     ECOLI      &lt;10  &lt;10  &lt;10   &lt;10  &lt;10&#34;     PC   &lt;10  &lt;10  &lt;10   &lt;10  &lt;10&#34;     PSA  &lt;10  &lt;10  &lt;10   &lt;10  &lt;10&#34;     SA   &lt;10  &lt;10  &lt;10   &lt;10  &lt;10__________________________________________________________________________Unpreserved controlOrganism48 Hours        7 Days              14 Days                    21 Days                           28 Days__________________________________________________________________________AN   89,000  32,000              22,000                    16,000 16,000CAN  210,000 670,000              430,000                    590,000                           640,000ECOLI640,000 360,000              410,000                    990,000                           68,000PC   19,000,000        3,200,000              7,000,000                    &gt;10,000                           2,760,000PSA  80      9,400 200,000                    &gt;10,000                           34,000SA   6,300,000        190,000              11,000                    580    120__________________________________________________________________________                  Inoculum Concentration                  Organism                       0 Hours                            21 Days__________________________________________________________________________                  AN   41,000                            32,000                  CAN  640  1,100,000                  ECOLI                       5,800,000                            1,300,000                  PC   900,000                            3,000,000                  PSA  1,800,000                            4,900,000                  SA   7,200,000                            2,000,000__________________________________________________________________________ 
    
     
                       TABLE 19______________________________________50:1 GERMAL ® II/IPBC______________________________________Test           48            14Level Organism Hours   7 Days                        Days  21 Days                                     28 Days______________________________________0.01% AN       4,100   40    &lt;10   &lt;10    &lt;10&#34;     CAN      310,000 7,700 560   5,600  5,200&#34;     ECOLI    170,000 710   10    &lt;10    120&#34;     PC       7,400   74,000                        340,000                              720,000                                     520,000&#34;     PSA      &lt;10     &lt;10   &lt;10   &lt;10    30&#34;     SA       110,000 11,000                        &lt;10   &lt;10    9,2000.05% AN       &lt;10     100   &lt;10   &lt;10    &lt;10&#34;     CAN      210     &lt;10   &lt;10   &lt;10    &lt;10&#34;     ECOLI    150,000 &lt;10   &lt;10   &lt;10    &lt;10&#34;     PC       &lt;10     &lt;10   &lt;10   &lt;10    &lt;10&#34;     PSA      &lt;10     &lt;10   &lt;10   &lt;10    &lt;10&#34;     SA       35,000  &lt;10   &lt;10   &lt;10    &lt;100.1%  AN       &lt;10     &lt;10   &lt;10   &lt;10    &lt;10&#34;     CAN      &lt;10     &lt;10   &lt;10   &lt;10    &lt;10&#34;     ECOLI    510     &lt;10   &lt;10   &lt;10    &lt;10&#34;     PC       &lt;10     &lt;10   &lt;10   &lt;10    &lt;10&#34;     PSA      &lt;10     &lt;10   &lt;10   &lt;10    &lt;10&#34;     SA       3,000   &lt;10   &lt;10   &lt;10    &lt;100.2%  AN       &lt;10     &lt;10   &lt;10   &lt;10    &lt;10&#34;     CAN      &lt;10     &lt;10   &lt;10   &lt;10    &lt;10&#34;     ECOLI    &lt;10     &lt;10   &lt;10   &lt;10    &lt;10&#34;     PC       &lt;10     &lt;10   &lt;10   &lt;10    &lt;10&#34;     PSA      &lt;10     &lt;10   &lt;10   &lt;10    &lt;10&#34;     SA       &lt;10     &lt;10   &lt;10   &lt;10    &lt;10______________________________________Unpreserved controlOrganism  48 Hours  7 Days   14 Days                            21 Days                                   28 Days______________________________________AN     89,000    32,000   22,000 16,000 16,000CAN    210,000   670,000  430,000                            590,000                                   640,000ECOLI  640,000   360,000  410,000                            990,000                                   68,000PC     19,000,000            3,200,000                     7,000,000                            &gt;10,000                                   2,760,000PSA    80        9,400    200,000                            &gt;10,000                                   34,000SA     6,300,000 190,000  11,000 580    120______________________________________           Inoculum Concentration           Organism                  0 Hours  21 Days______________________________________           AN     41,000   32,000           CAN    640      1,100,000           ECOLI  5,800,000                           1,300,000           PC     900,000  3,000,000           PSA    1,800,000                           4,900,000           SA     7,200,000                           2,000,000______________________________________ 
    
     
                                           TABLE 20__________________________________________________________________________20:1 GERMALL ® II/IPBC__________________________________________________________________________Test Level Organism      48 Hours           7 Days                14 Days                     21 Days                           28 Days__________________________________________________________________________0.01% AN   3,100           &lt;10  &lt;10  &lt;10   &lt;10&#34;     CAN  75,000           220  &lt;10  &lt;10   2,400&#34;     ECOLI      160,000           110  &lt;10  &lt;10   20&#34;     PC   12,000           1,000,000                2,100,000                     &gt;1,000,000                           730,000&#34;     PSA  &lt;10  &lt;10  &lt;10  &lt;10   4,000&#34;     SA   140,000           4,100                &lt;10  &lt;10   1,6800.05% AN   &lt;10  &lt;10  &lt;10  &lt;10   &lt;10&#34;     CAN  &lt;10  &lt;10  &lt;10  &lt;10   &lt;10&#34;     ECOLI      16,000           &lt;10  &lt;10  &lt;10   &lt;10&#34;     PC   &lt;10  &lt;10  &lt;10  &lt;10   &lt;10&#34;     PSA  &lt;10  &lt;10       &lt;10   &lt;10&#34;     SA   31,000           &lt;10  &lt;10  &lt;10   &lt;100.1%  AN   &lt;10  &lt;10  &lt;10  &lt;10   &lt;10&#34;     CAN  &lt;10  &lt;10  &lt;10  &lt;10   &lt;10&#34;     ECOLI      &lt;10  &lt;10  &lt; 10 &lt;10   &lt;10&#34;     PC   &lt;10  &lt;10  &lt;10  &lt;10   &lt;10&#34;     PSA  &lt;10  &lt;10  &lt;10  &lt;10   &lt;10&#34;     SA   6,800           &lt;10  &lt;10  &lt;10   &lt;100.2%  AN   &lt;10  &lt;10  &lt;10  &lt;10   &lt;10&#34;     CAN  &lt;10  &lt;10  &lt;10  &lt;10   &lt;10&#34;     ECOLI      &lt;10  &lt;10  &lt;10  &lt;10   &lt;10&#34;     PC   &lt;10  &lt;10  &lt;10  &lt;10   &lt;10&#34;     PSA  &lt;10  &lt;10  &lt;10  &lt;10   &lt;10&#34;     SA   &lt;10  &lt;10  &lt;10  &lt;10   &lt;10__________________________________________________________________________Unpreserved controlOrganism48 Hours       7 Days             14 Days                    21 Days                          28 Days__________________________________________________________________________AN   89,000 32,000             22,000 16,000                          16,000CAN  210,000       670,000             430,000                    590,000                          640,000ECOLI640,000       360,000             410,000                    990,000                          68,000PC   19,000,000       3,200,000             7,000,000                    &gt;10,000                          2,760,000PSA  80     9,400 200,000                    &gt;10,000                          34,000SA   6,300,000       190,000             11,000 580   120__________________________________________________________________________                 Inoculum Concentration                 Organism                      0 Hours                           21 Days__________________________________________________________________________                 AN   41,000                           32,000                 CAN  640  1,100,000                 ECOLI                      5,800,000                           1,300,000                 PC   900,000                           3,000,000                 PSA  1,800,000                           4,900,000                 SA   7,200,000                           2,000,000__________________________________________________________________________ 
    
     
                                           TABLE 21__________________________________________________________________________2000:1 DMDMH/IPBC__________________________________________________________________________Test Level  Organism       48 Hours            7 Days                 14 Days                      21 Days                           28 Days__________________________________________________________________________0.025% AN   4,100            38,000                 270  80   100&#34;      CAN  270,000            1,900,000                 550,000                      220,000                           210,000&#34;      ECOLI       1,300,000            &lt;10  &lt;10  &lt;10  3,000&#34;      PC   &lt;10  &lt;10  &lt;10  &lt;10  &lt;10&#34;      PSA  &lt;10  &lt;10  &lt;10  &lt;10  &lt;10&#34;      SA   42,000            20   &lt;10  &lt;10  &lt;100.05%  AN   &lt;10  &lt;10  &lt;10  &lt;10  &lt;10&#34;      CAN  770,000            130,000                 670,000                      160,000                           64,000&#34;      ECOLI       220,000            &lt;10  &lt;10  &lt;10  &lt;10&#34;      PC   &lt;10  &lt;10  &lt;10  &lt;10  &lt;10&#34;      PSA  &lt;10  &lt;10  &lt;10  &lt;10  &lt;10&#34;      SA   9,000            &lt;10  &lt;10  &lt;10  &lt;100.1%   AN   &lt;10  &lt;10  &lt;10  &lt;10  &lt;10&#34;      CAN  580  840  500  40,000                           83,000&#34;      ECOLI       340  &lt;10  &lt;10  &lt;10  &lt;10&#34;      PC   &lt;10  &lt;10  &lt;10  &lt;10  &lt;10&#34;      PSA  &lt;10  &lt;10  &lt;10  &lt;10  &lt;10&#34;      SA   29,000            &lt;10  &lt;10  &lt;10  &lt;100.2%   AN   &lt;10  &lt;10  &lt;10  &lt;10  &lt;10&#34;      CAN  &lt;10  10   10   &lt;10  21&#34;      ECOLI       &lt;10  &lt;10  &lt;10  &lt;10  &lt;10&#34;      PC   &lt;10  &lt;10  &lt;10  &lt;10  &lt;10&#34;      PSA  &lt;10  &lt;10  &lt;10  &lt;10  &lt;10&#34;      SA   &lt;10  &lt;10  &lt;10  &lt;10  &lt;10__________________________________________________________________________Unpreserved controlOrganism48 Hours       7 Days 14 Days                    21 Days                          28 Days__________________________________________________________________________AN   37,000 36,000 24,000                    5,200 6,000CAN  120,000       1,900,000              3,300,000                    480,000                          790,000ECOLI150,000       2,500,000              7,300,000                    240,000                          140,000PC   19,000,000       15,600,000              5,900,000                    8,500,000                          31,000,000PSA  &lt;10    &lt;10    100   15,200                          300,000SA   7,000,000       &gt;1,000,000              12,000                    3,000 110__________________________________________________________________________                 Inoculum Concentration                 Organism                      0 Hours                           21 Days__________________________________________________________________________                 AN   50,000                           41,000                 CAN  1,400,000                           640                 ECOLI                      4,800,000                           5,800,000                 PC   9,200,000                           900,000                 PSA  6,900,000                           9,000,000                 SA   5,700,000                           7,200,000__________________________________________________________________________ 
    
     
                                           TABLE 22__________________________________________________________________________1000:1 DMDMH/IPBC__________________________________________________________________________Test Level  Organism       48 Hours            7 Days                 14 Days                      21 Days                           28 Days__________________________________________________________________________0.025% AN   650  900  &lt;10  &lt;10  140&#34;      CAN  97,000            900,000                 2,300,000                      120,000                           120,000&#34;      ECOLI       160,000            &lt;10  &lt;10  &lt;10  2,400&#34;      PC   &lt;10  &lt;10  &lt;10  &lt;10  &lt;10&#34;      PSA  &lt;10  &lt;10  &lt;10  &lt;10  &lt;10&#34;      SA   23,000            &lt;10  &lt;10  &lt;10  900.05%  AN   20   &lt;10  &lt;10  &lt;10  &lt;10&#34;      CAN  65,000            520,000                 56,000                      32,000                           370,000&#34;      ECOLI       26,000            &lt;10  &lt;10  &lt;10  &lt;10&#34;      PC   &lt;10  &lt;10  &lt;10  &lt;10  &lt;10&#34;      PSA  &lt;10  &lt;10  &lt;10  &lt;10  &lt;10&#34;      SA   12,000            &lt;10  &lt;10  &lt;10  &lt;100.1%   AN   &lt;10  &lt;10  &lt;10  &lt;10  &lt;10&#34;      CAN  3,100            4,800                 180  640  4,400&#34;      ECOLI       45,000            &lt;10  &lt;10  &lt;10  &lt;10&#34;      PC   &lt;10  &lt; 10 &lt;10  &lt;10  &lt;10&#34;      PSA  &lt;10  &lt;10  &lt;10  &lt;10  &lt;10&#34;      SA   6,000            &lt;10  &lt;10  &lt;10  &lt;100.2%   AN   &lt;10  &lt;10  &lt;10  &lt;10  &lt;10&#34;      CAN  &lt;10  &lt;10  &lt;10  &lt;10  &lt;10&#34;      ECOLI       &lt;10  &lt;10  &lt;10  &lt;10  &lt;10&#34;      PC   &lt;10  &lt;10  &lt;10  &lt;10  &lt;10&#34;      PSA  &lt;10  &lt;10  &lt;10  &lt;10  &lt;10&#34;      SA   180  &lt;10  &lt;10  &lt;10  &lt;10__________________________________________________________________________Unpreserved controlOrganism48 Hours       7 Days 14 Days                    21 Days                          28 Days__________________________________________________________________________AN   37,000 36,000 24,000                    5,200 6,000CAN  120,000       1,900,000              3,300,000                    480,000                          790,000ECOLI150,000       2,500,000              7,300,000                    240,000                          140,000PC   19,000,000       15,600,000              5,900,000                    8,500,000                          31,000,000PSA  &lt;10    &lt;10    100   15,200                          300,000SA   7,000,000       &gt;1,000,000              12,000                    3,000 110__________________________________________________________________________                 Inoculum Concentration                 Organism                      0 Hours                           21 Days__________________________________________________________________________                 AN   50,000                           41,000                 CAN  1,400,000                           640                 ECOLI                      4,800,000                           5,800,000                 PC   9,200,000                           900,000                 PSA  6,900,000                           9,000,000                 SA   5,700,000                           7,200,000__________________________________________________________________________ 
    
     
                                           TABLE 23__________________________________________________________________________2000:1 GADM/IPBC__________________________________________________________________________Test Level  Organism       48 Hours            7 Days                 14 Days                      21 Days                           28 Days__________________________________________________________________________0.025% AN   300  21   10   &lt;10  11&#34;      CAN  480,000            890,000                 940,000                      1,040,000                           130,000&#34;      ECOLI       230,000            &lt;10  &lt;10  &lt;10  &lt;10&#34;      PC   &lt;10  &lt;10  &lt;10  &lt;10  &lt;10&#34;      PSA  &lt;10  &lt;10  &lt;10  &lt;10  &lt;10&#34;      SA   78,000            &lt;10  &lt;10  &lt;10  &lt;100.05%  AN   &lt;10  &lt;10  &lt;10  &lt;10  &lt;10&#34;      CAN  110,000            4,100,000                 3,600,000                      330,000                           97,000&#34;      ECOLI       120,000            &lt;10  &lt;10  &lt;10  &lt;10&#34;      PC   &lt;10  &lt;10  &lt;10  &lt;10  &lt;10&#34;      PSA  &lt;10  &lt;10  &lt;10  &lt;10  &lt;10&#34;      SA   56,000            &lt;10  &lt;10  &lt;10  &lt;100.1%   AN   &lt;10  &lt;10  &lt;10  &lt;10  &lt;10&#34;      CAN  1,200,000            53,000                 430,000                      144,000                           110,000&#34;      ECOLI       &lt;10  &lt;10  &lt;10  &lt;10  &lt;10&#34;      PC   &lt;10  &lt;10  &lt;10  &lt;10  &lt;10&#34;      PSA  &lt;10  &lt;10  &lt;10  &lt;10  &lt;10&#34;      SA   &lt;10  &lt;10  &lt;10  &lt;10  &lt;100.2%   AN   &lt;10  &lt;10  &lt;10  &lt;10  &lt;10&#34;      CAN  90,000            &lt;10  &lt;10  &lt;10  60&#34;      ECOLI       &lt;10  &lt;10  &lt;10  &lt;10  &lt;10&#34;      PC   &lt;10  &lt;10  &lt;10  &lt;10  &lt;10&#34;      PSA  &lt;10  &lt;10  &lt;10  &lt;10  &lt;10&#34;      SA   &lt;10  &lt;10  &lt;10  &lt;10  &lt;10__________________________________________________________________________Unpreserved controlOrganism48 Hours       7 Days             14 Days                    21 Days                          28 Days__________________________________________________________________________AN   50,000 33,000             33,000 13,000                          5,400CAN  780,000       780,000             780,000                    500,000                          170,000ECOLI600,000       3,100,000             920,000                    920,000                          140,000PC   11,000,000       30,000,000             10,000,000                    10,000,000                          1,400,000PSA  3,800  600   12,800 12,800                          100,000SA   14,000,000       410,000             7,100  7,100 80__________________________________________________________________________                 Inoculum Concentration                 Organism                      0 Hours                           21 Days__________________________________________________________________________                 AN   53,000                           10,000                 CAN  1,900,000                           310,000                 ECOLI                      170,000                           3,500,000                 PC   87,000                           2,500,000                 PSA  390,000                           5,400,000                 SA   200,000                           4,100,000__________________________________________________________________________ 
    
     Discussion of Challenge Testing Results 
     The 28-day challenge results reported in Tables 15-23 above demonstrate the effectiveness of the preservative admixture of the invention in a use emulsion composition against a wide range of bacteria and fungi organisms. 
     For example, admixture compositions of Germall® II and IPBC at a wt. ratio of 2000:1 (Table 15), when present at use levels of 0.05 to 0.2%, corresponding to 0.75 to 10 ppm IPBC and 500 to 2000 ppm methylol levels, provide substantially complete protection against all tested organisms after 28 days. At the low use level of 0.05% active, all the challenge tests passed within 21 days. Then, upon reinoculation after 21 days, all organisms died within 7 days except CAN which cleared within 14 days. 
     Table 21 shows the challenge test results for DMDMH and IPBC admixtures at the same 2000:1 wt. ratio. A use level of 0.2%, however, is needed for this blend to pass against all organisms after 21 days. Upon reinoculation, all organisms died within 7 days with the exception of CAN which cleared within 14 days. It is thus evident from these results that Germall® II blended with IPBC is 4 times more effective than a DMDMH/IPBC blend. 
     Germall® II also is superior to GADM as the methylol compound, as shown in Table 23. 
     While the invention has been described with particular reference to certain embodiments thereof, it will be understood that changes and modifications may be made which are within the skill of the art. Accordingly, it is intended to be bound only by the following claims, in which: