Abstract:
A water soluble preservative admixture of biocidal compounds for addition to commercial use compositions at predetermined use levels, and uniformly distributed therein, to provide long-time synergistic biocidal activity against a wide range of fungi and both gram-negative and gram-positive bacteria, which comprises powders of 
     (a) one or more methylol compounds, or their equivalents, and 
     (b) iodopropynyl alcohol, or its ester, carbamate or ether derivative thereof, 
     in a weight ratio of (a):(b) of 100:1 to 2000:1.

Description:
BACKGROUND OF THE INVENTION 
     1. Field of the Invention 
     This invention relates to a water soluble preservative admixture for addition to commercial use formulations to provide long time synergistic biocidal activity therein, and, more particularly, to admixtures of a methylol compound and an iodopropynyl compound, in predetermined weight ratios of 100:1 to 2000:1. 
     2. Description of the Prior Art 
     Combinations of antimicrobial agents have been developed in the prior art in order to: 
     (1) produce a biochemical synergism; 
     (2) broaden the antimicrobial spectrum of activity of each agent; 
     (3) increase water solubility for the admixture; 
     (4) minimize the toxicity or irritation of a given agent to the host; and 
     (5) minimize physical and chemical incompatibilities. 
     True biological synergism exists when two agents, when combined, require lesser amounts of the agents to bring about the same inhibitory or cidal effect than either single agent alone. While synergistic interaction for two or more antimicrobial agents does produce more than merely an additive effect in the resultant biological activity, in most cases the mechanism of such synergism remains a mystery. 
     M. Rosen et al., in U.S. Pat. No. 4,844,891, for example, described a preservative admixture of (a) a formaldehyde donor and (b) a halopropynyl compound, in a weight ratio of (a):(b) of 50:1 to 1:1, preferably 30:1 to 2:1, and, most preferably, 20:1 to 10:1, as providing fungicidal activity for 1-3 days in commercial use formulations. However, Rosen observed that when the ratio of (a):(b) in the concentrate exceeded 50:1 (System No. 16 in Table 1, a ratio of 73.33), the preservative composition was ineffective in providing biocidal protection in the use formulations. Thus a relatively large amount of the halopropynyl compound was required by Rosen to provide significant biocidal activity in the use composition. In such admixtures, although the formaldehyde donor is water soluble, the halopropynyl compound is substantially insoluble in water. Therefore it was difficult for Rosen to uniformly distribute his admixture throughout the use composition. 
     For these and other reasons, it is desired to provide a new and improved water soluble preservative admixture of such biocidal compounds which requires relatively little of the water insoluble and expensive halopropynyl compound, and that also provides effective synergistic protection in use formulations against a wide range of fungi and bacteria at different use levels over a long period of time. 
     A feature of the present invention is the provision of an admixture concentrate which is water soluble and which therefore can be uniformly distributed in use compositions at a predetermined use level. 
     Another feature herein is the provision of a preservative admixture which exhibits a long term synergistic biocidal activity against wide range of fungi and both gram-negative and gram-positive bacteria in the use compositions. 
     Still another feature of this invention is the provision of a water soluble preservative admixture for personal care compositions in the form of a solution, lotion, gel, emulsion, emulsifiable concentrate, suspension, slurry or cream. 
     These and other objects and features of the invention will be made apparent from the following more particular description of the invention. 
     SUMMARY OF THE INVENTION 
     What has been discovered is a water soluble preservative composition for addition to commercial use compositions at predetermined use levels, and uniformly distributed therein, which provides long term synergistic biocidal activity against a wide range of fungi and both gram-negative and gram-positive bacteria. The composition of the invention comprises an admixture of powders of 
     (a) a methylol compound, or their equivalent, and 
     (b) iodopropynyl alcohol, or its ester, carbamate or ether derivative thereof, 
     in a weight ratio of (a):(b) of 100:1 to 2000:1, preferably 200:1 to 500:1. 
     Commercial use compositions containing about 0.01 to 2% by weight of the composition of the invention also are provided therein. Such use compositions contain an iodopropynyl compound in an amount of about 0.5 to 10 ppm, to provide the desired antifungal activity, and a methylol compound, or equivalent thereof, in an amount of at least 250 ppm, to provide the desired antibacterial activity. 
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     The invention is based upon the discoveries that in a predetermined admixture of (a) a methylol compound, or its equivalent, and (b) an iodopropynyl compound: 
     (1) Iodopropynyl compounds are substantially water insoluble at weight ratios of (a):(b) of less than 100; accordingly, at weight ratios below 100:1, it is difficult to uniformly distribute the iodopropynyl compound in aqueous use compositions, particularly in creams, gels and the like. In this invention, the admixtures are used at a weight ratio of (a):(b) of 100:1 to 2000:1, which are water soluble in all use compositions at conventional use levels. 
     (2) Effective synergistic biocidal activity is achieved for admixtures having a weight ratio of (a):(b) of 100:1 to 2000:1. Such admixtures have a Synergistic Index (SI) value approaching zero (maximum synergism) for a wide range of organisms. In contrast, admixtures with (a):(b) ratios below 100:1, e.g. 10:1 to 50:1, are much less synergistic, and are active with only a narrower range of organisms. 
     (3) Preservative activity for use compositions is achieved most effectively with an admixture wt. ratio of 100:1 to 2000:1 at use levels of 0.05 to 2% by weight of the finished product. In this amount, the iodopropynyl compound is present in an amount of only 0.5-10 ppm, which significantly reduces the cost and toxicity of the use composition. The methylol compound also is present in an amount of at least 250 ppm. 
     The experimental results upon which these discoveries are based are described below. In these examples, the (a) methylol compound may be selected from diazolidinyl urea. (GERMALL® II) N-[1,3-bis (hydroxymethyl)-2,5-dioxo-4-imidazolidinyl]-N,N&#39;-bis(hydroxymethyl) urea, imidurea (GERMALL® 115), 1,3-dimethylol-5,5-dimethyl hydantoin (DMDMH), sodium hydroxymethylglycinate (SUTTOCIDE® A), glycine anhydride dimethylol (GADM), dimethylhydroxymethyl pyrazole, (1-(3-chloroallyl)-3,5,7-triaza-1-azoniaadamantane chloride (a methylol equivalent),1,3,5-(trishydroxyethyl)hexahydrotriazine, or hydroxymethyl pyrrolidone; and the (b) iodopropynyl compound is iodopropynyl alcohol (IPGA) or 3-iodo-2-propynylbutyl carbamate (IPBC). 
     1. WATER SOLUBILITY 
     The water solubility or insolubility of admixtures of several methylol compounds with IPBC as 1% aqueous solutions is shown in Tables A through C below. 
     
                       TABLE A______________________________________Weight Ratio of   AmountGermall ® II:IPBC             in ppm   Solubility______________________________________2000:1             5       Soluble1000:1             10      Soluble500:1              20      Soluble200:1              50      Soluble100:1             100      Soluble 50:1             200      Insoluble 20:1             500      Insoluble______________________________________ 
    
     
                       TABLE B______________________________________Weight Ratio of AmountGADM: IPBC      in ppm   Solubility______________________________________2000:1           5       Soluble1000:1          10       Soluble 500:1          20       Soluble 500:1          50       Soluble 100:1          100      Soluble 50:1           200      Insoluble 20:1           500      Insoluble______________________________________ 
    
     
                       TABLE C______________________________________Weight Ratio of  AmountDMDMH: IPBC      in ppm   Solubility______________________________________2000:1            5       Soluble1000:1           10       Soluble500:1            20       Soluble200:1            50       Soluble100:1            100      Soluble 50:1            200      Insoluble 20:1            500      Insoluble______________________________________ 
    
     These results demonstrate that admixtures having a ratio of 50:1 or 20:1 are insoluble in water whereas at ratios of 100:1 to 2000:1 the admixtures are soluble in water. 
     2. SYNERGISM 
     Tables 1 through 14 below demonstrate the very effective synergistic interaction between compounds &#34;a&#34; and &#34;b&#34; against a broad range of fungi and both gram-negative and gram-positive bacteria. The following organisms were tested: 
     
         ______________________________________        ATCCOrganism     Number   Inoculum Concentration______________________________________Ps. aeruginosa (PSA)*         9027    2.1 × 10.sup.6                          CFU/gm of ProductE. coli (ECOLI)*         8739    4.7 × 10.sup.5                          CFU/gm of ProductStaph. aureus (SA)**         6538    1.6 × 10.sup.6                          CFU/gm of ProductPs. cepacia (PC)*        25416    1.6 × 10.sup.6                          CFU/gm of ProductC. albicans (CAN)***        10231    8.0 × 10.sup.4                          CFU/gm of ProductA. niger (AN)***        16404    2.7 × 10.sup.5                          CFU/gm of Product______________________________________ 
    
     
                                           TABLE D__________________________________________________________________________Static (MIC) and Cidal Activities of Several Antimicrobial Compounds(Static/Cidal Concentrations in ppm)Organism (ATCC #)      IPBC  Germall ® II                   GADM  DMDMH IPGA__________________________________________________________________________(SA) (6538)      100/200            400/1600                   400/800                         450/1600                               300/5000(ECOLI) (8739)       50/100            400/1600                   400/800                         400/800                               150/600(PSA) (9027)       800/1200            400/1600                   400/400                         600/1600                               70/70(PC) (25416)      1200/1800            200/400                   200/400                         600/1600                               70/300(CAN) (10231)       50/100            1500/15000                    7500/15000                         8000/16000                               50/300(AN) (16404)       50/100            3200/3200                   1600/3200                         8000/16000                               30/30__________________________________________________________________________ 
    
     The Synergism Index was determined by the same mathematical treatment of such data described by Kull et al. in Applied Microbiology 9,538-541 (1961) using the following relationship: ##EQU1## where: 1. Q a  =The quantity of Compound a acting alone, producing an endpoint. 
     2. Q b  =The quantity of Compound b acting alone, producing and endpoint. 
     3. Q A  =The quantity of Compound A in mixture, producing an endpoint. 
     4. Q B  =The quantity of Compound in mixture, producing an endpoint. 
     When SI is equal to 1a mere additive effect of the components in the mixture is indicated; when SI is less than 1, synergism has occurred; and when SI is greater than 1 it indicates antagonism of the two components. 
     According to this well known method of measuring synergism, the quantity of each component in the various mixtures is compared with the, quantity of pure component that is required to reach the same endpoint or to produce the same microbiological effect as the mixture. 
     
                       TABLE 1______________________________________2000:1 Wt. Ratio GERMALL ® II/IPBCUse Level   Organism Q.sub.a Q.sub.b                          Q.sub.A                                Q.sub.B                                       SI______________________________________0.01%   SA       200     1600  0.05  99.95  0.06&#34;       ECOLI    100     1600  0.05  99.95  0.06&#34;       PSA      1200    1600  0.05  99.95  0.06&#34;       PC       1800    1250  0.05  99.95  0.08&#34;       CAN      100     15000 0.05  99.95  0.01&#34;       AN       100     3200  0.05  99.95  0.03 0.025% SA       200     1600  0.125 249.9  0.16&#34;       ECOLI    100     1600  0.125 249.9  0.16&#34;       PSA      1200    1600  0.125 249.9  0.16&#34;       PC       1800    1250  0.125 249.9  0.20&#34;       CAN      100     15000 0.125 249.9  0.02&#34;       AN       100     3200  0.125 249.9  0.080.05%   SA       200     1600  0.25  499.8  0.31&#34;       ECOLI    100     1600  0.25  499.8  0.31&#34;       PSA      1200    1600  0.25  499.8  0.31&#34;       PC       1800    1250  0.25  499.8  0.40&#34;       CAN      100     15000 0.25  499.8  0.04&#34;       AN       100     3200  0.25  499.8  0.160.10%   SA       200     1600  0.5   999.5  0.63&#34;       ECOLI    100     1600  0.5   999.5  0.63&#34;       PSA      1200    1600  0.5   999.5  0.63&#34;       PC       1800    1250  0.5   999.5  0.80&#34;       CAN      100     15000 0.5   999.5  0.07&#34;       AN       100     3200  0.5   999.5  0.320.20%   SA       200     1600  1     1999   1.25&#34;       ECOLI    100     1600  1     1999   1.26&#34;       PSA      1200    1600  1     1999   1.25&#34;       PC       1800    1250  1     1999   1.60&#34;       CAN      100     15000 1     1999   0.14&#34;       AN       100     3200  1     1999   0.630.40%   SA       200     1600  2     3998   2.51&#34;       ECOLI    100     1600  2     3998   2.52&#34;       PSA      1200    1600  2     3998   2.50&#34;       PC       1800    1250  2     3998   3.20&#34;       CAN      100     15000 2     3998   0.29&#34;       AN       100     3200  2     3998   1.270.50%   SA       200     1600  2.5   4997.5 3.14&#34;       ECOLI    100     1600  2.5   4997.5 3.15&#34;       PSA      1200    1600  2.5   4997.5 3.13&#34;       PC       1800    1250  2.5   4997.5 4.00&#34;       CAN      100     15000 2.5   4997.5 0.36-       AN       100     3200  2.5   4997.5 1.59______________________________________ 
    
     
                       TABLE 2______________________________________1000:1 Wt. Ratio GERMALL ® II/IPBCUse Level   Organism Q.sub.a Q.sub.b                           Q.sub.A                                Q.sub.B                                       SI______________________________________0.01%   SA       200     1600   0.1  99.9   0.06&#34;       ECOLI    100     1600   0.1  99.9   0.06&#34;       PSA      1200    1600   0.1  99.9   0.06&#34;       PC       1800    1250   0.1  99.9   0.08&#34;       CAN      100     15000  0.1  99.9   0.01&#34;       AN       100     3200   0.1  99.9   0.03 0.025% SA       200     1600   0.25 249.8  0.16&#34;       ECOLI    100     1600   0.25 249.8  0.16&#34;       PSA      1200    1600   0.25 249.8  0.16&#34;       PC       1800    1250   0.25 249.8  0.20&#34;       CAN      100     15000  0.25 249.8  0.02&#34;       AN       100     3200   0.25 249.8  0.080.05%   SA       200     1600   0.5  499.5  0.31&#34;       ECOLI    100     1600   0.5  499.5  0.32&#34;       PSA      1200    1600   0.5  499.5  0.31&#34;       PC       1800    1250   0.5  499.5  0.40&#34;       CAN      100     15000  0.5  499.5  0.04&#34;       AN       100     3200   0.5  499.5  0.160.10%   SA       200     1600   1    999    0.63&#34;       ECOLI    100     1600   1    999    0.63&#34;       PSA      1200    1600   1    999    0.63&#34;       PC       1800    1250   1    999    0.80&#34;       CAN      100     15000  1    999    0.08&#34;       AN       100     3200   1    999    0.320.20%   SA       200     1600   2    1998   1.26&#34;       ECOLI    100     1600   2    1998   1.27&#34;       PSA      1200    1600   2    1998   1.25&#34;       PC       isoo    1250   2    1998   1.60&#34;       CAN      100     15000  2    1998   0.15&#34;       AN       100     3200   2    1998   0.640.40%   SA       200     1600   4    3996   2.52&#34;       ECOLI    100     1600   4    3996   2.54&#34;       PSA      1200    1600   4    3996   2.50&#34;       PC       1800    1250   4    3996   3.20&#34;       CAN      100     15000  4    3996   0.31&#34;       AN       100     3200   4    3996   1.290.50%   SA       200     1600   5    4995   3.15&#34;       ECOLI    100     1600   5    4995   3.17&#34;       PSA      1200    1600   5    4995   3.13&#34;       PC       1800    1250   5    4995   4.00&#34;       CAN      100     15000  5    4995   0.38&#34;       AN       100     3200   5    4995   1.61______________________________________ 
    
     
                       TABLE 3______________________________________500:1 Wt. Ratio GERMALL ® II/IPBCUse Level   Organism Q.sub.a Q.sub.b                           Q.sub.A                                Q.sub.B                                       SI______________________________________0.01%   SA       200     1600   0.2  99.8   0.06&#34;       ECOLI    100     1600   0.2  99.8   0.06&#34;       PSA      1200    1600   0.2  99.8   0.06&#34;       PC       1800    1250   0.2  99.8   0.08&#34;       CAN      100     15000  0.2  99.8   0.01&#34;       AN       100     3200   0.2  99.8   0.03 0.025% SA       200     1600   0.5  249.5  0.16&#34;       ECOLI    100     1600   0.5  249.5  0.16&#34;       PSA      1200    1600   0.5  249.5  0.16&#34;       PC       1800    1250   0.5  249.5  0.20&#34;       CAN      100     15000  0.5  249.5  0.02&#34;       AN       100     3200   0.5  249.5  0.080.05%   SA       200     1600   1    499    0.32&#34;       ECOLI    100     1600   1    499    0.32&#34;       PSA      1200    1600   1    499    0.31&#34;       PC       1800    1250   1    499    0.40&#34;       CAN      100     15000  1    499    0.04&#34;       AN       100     3200   1    499    0.170.10%   SA       200     1600   2    998    0.63&#34;       ECOLI    100     1600   2    998    0.64&#34;       PSA      1200    1600   2    998    0.63&#34;       PC       1800    1250   2    998    0.80&#34;       CAN      100     15000  2    998    0.09&#34;       AN       100     3200   2    998    0.330.20%   SA       200     1600   4    1996   1.27&#34;       ECOLI    100     1600   4    1996   1.29&#34;       PSA      1200    1600   4    1996   1.25&#34;       PC       1800    1250   4    1996   1.60&#34;       CAN      100     15000  4    1996   0.17&#34;       AN       100     3200   4    1996   0.660.40%   SA       200     1600   8    3992   2.54&#34;       ECOLI    100     1600   8    3992   2.58&#34;       PSA      1200    1600   8    3992   2.50&#34;       PC       1800    1250   8    3992   3.20&#34;       CAN      100     15000  8    3992   0.35&#34;       AN       100     3200   8    3992   1.330.50%   SA       200     1600   10   4990   3.17&#34;       ECOLI    100     1600   10   4990   3.22&#34;       PSA      1200    1600   10   4990   3.13&#34;       PC       1800    1250   10   4990   4.00&#34;       CAN      100     15000  10   4990   0.43&#34;       AN       100     3200   10   4990   1.66______________________________________ 
    
     
                       TABLE 4______________________________________200:1 Wt. Ratio GERMALL ® II/IPBCUse Level   Organism Q.sub.a Q.sub.b                           Q.sub.A                                Q.sub.B                                       SI______________________________________0.01%   SA       200     1600   0.5  99.5   0.06&#34;       ECOLI    100     1600   0.5  99.5   0.07&#34;       PSA      1200    1600   0.5  99.5   0.06&#34;       PC       1800    1250   0.5  99.5   0.08&#34;       CAN      100     15000  0.5  99.5   0.01&#34;       AN       100     3200   0.5  99.5   0.04 0.025% SA       200     1600   1.25 248.75 0.16&#34;       ECOLI    100     1600   1.25 248.75 0.17&#34;       PSA      1200    1600   1.25 248.75 0.16&#34;       PC       1800    1250   1.25 248.75 0.20&#34;       CAN      100     15000  1.25 248.75 0.03&#34;       AN       100     3200   1.25 248.75 0.090.05%   SA       200     1600   2.5  497.5  0.32&#34;       ECOLI    100     1600   2.5  497.5  0.34&#34;       PSA      1200    1600   2.5  497.5  0.31&#34;       PC       1800    1250   2.5  497.5  0.40&#34;       CAN      100     15000  2.5  497.5  0.06&#34;       AN       100     3200   2.5  497.5  0.180.10%   SA       200     1600   5    995    0.65&#34;       ECOLI    100     1600   5    995    0.67&#34;       PSA      1200    1600   5    995    0.63&#34;       PC       1800    1250   5    995    0.80&#34;       CAN      100     15000  5    995    0.12&#34;       AN       100     3200   5    995    0.360.20%   SA       200     1600   10   1990   1.29&#34;       ECOLI    100     1600   10   1990   1.34&#34;       PSA      1200    1600   10   1990   1.25&#34;       PC       1800    1250   10   1990   1.60&#34;       CAN      100     15000  10   1990   0.23&#34;       AN       100     3200   10   1990   0.720.40%   SA       200     1600   20   3980   2.59&#34;       ECOLI    100     1600   20   3980   2.69&#34;       PSA      1200    1600   20   3980   2.50&#34;       PC       1800    1250   20   3980   3.20&#34;       CAN      100     15000  20   3980   0.47&#34;       AN       100     3200   20   3980   1.440.50%   SA       200     1600   25   4975   3.23&#34;       ECOLI    100     1600   25   4975   3.36&#34;       PSA      1200    1600   25   4975   3.13&#34;       PC       1800    1250   25   4975   3.99&#34;       CAN      100     15000  25   4975   0.58&#34;       AN       100     3200   25   4975   1.80______________________________________ 
    
     
                       TABLE 5______________________________________100:1 Wt. Ratio GERMALL ® II/IPBCUse Level   Organism  Q.sub.a Q.sub.b                            Q.sub.A                                 Q.sub.B                                       SI______________________________________0.01%   SA        200     1600   1     99   0.06&#34;       ECOLI     100     1600   1     99   0.07&#34;       PSA       1200    1600   1     99   0.06&#34;       PC        1800    1250   1     99   0.08&#34;       CAN       100     15000  1     99   0.02&#34;       AN        100     3200   1     99   0.04 0.025% SA        200     1600   2.5   248  0.17&#34;       ECOLI     100     1600   2.5   248  0.18&#34;       PSA       1200    1600   2.5   248  0.16&#34;       PC        1800    1250   2.5   248  0.20&#34;       CAN       100     15000  2.5   248  0.04&#34;       AN        100     3200   2.5   248  0.100.05%   SA        200     1600   5     495  0.33&#34;       ECOLI     100     1600   5     495  0.36&#34;       PSA       1200    1600   5     495  0.31&#34;       PC        1800    1250   5     495  0.40&#34;       CAN       100     15000  5     495  0.08&#34;       AN        100     3200   5     495  0.200.10%   SA        200     1600   10    990  0.67&#34;       ECOLI     100     1600   10    990  0.72&#34;       PSA       1200    1600   10    990  0.63&#34;       PC        1800    1250   10    990  0.80&#34;       CAN       100     15000  10    990  0.17&#34;       AN        100     3200   10    990  0.410.20%   SA        200     1600   20   1980  1.34&#34;       ECOLI     100     1600   20   1980  1.44&#34;       PSA       1200    1600   20   1980  1.25&#34;       PC        1800    1250   20   1980  1.60&#34;       CAN       100     15000  20   1980  0.33&#34;       AN        100     3200   20   1980  0.820.40%   SA        200     1600   40   3960  2.68&#34;       ECOLI     100     1600   40   3960  2.88&#34;       PSA       1200    1600   40   3960  2.51&#34;       PC        1800    1250   40   3960  3.19&#34;       CAN       100     15000  40   3960  0.66&#34;       AN        100     3200   40   3960  1.640.50%   SA        200     1600   50   4950  3.34&#34;       ECOLI     100     1600   50   4950  3.59&#34;       PSA       1200    1600   50   4950  3.14&#34;       PC        1800    1250   50   4950  3.99&#34;       CAN       100     15000  50   4950  0.83&#34;       AN        100     3200   50   4950  2.05______________________________________ 
    
     
                       TABLE 6______________________________________50:1 Wt. Ratio GERMALL ® II/IPBCUse Level   Organism Q.sub.a Q.sub.b                          Q.sub.A                                Q.sub.B                                      SI______________________________________0.01%   SA       200     1600   2     98   0.070.01%   ECOLI    100     1600   2     98   0.080.01%   PSA      1200    1600   2     98   0.060.01%   PC       1800    1250   2     98   0.080.01%   CAN      100     15000  2     98   0.030.01%   AN       100     3200   2     98   0.05 0.025% SA       200     1600   5    245   0.18 0.025% ECOLI    100     1600   5    245   0.20 0.025% PSA      1200    1600   5    245   0.16 0.025% PC       1800    1250   5    245   0.20 0.025% CAN      100     15000  5    245   0.07 0.025% AN       100     3200   5    245   0.130.05%   SA       200     1600  10    490   0.360.05%   ECOLI    100     1600  10    490   0.410.05%   PSA      1200    1600  10    490   0.310.05%   PC       1800    1250  10    490   0.400.05%   CAN      100     15000 10    490   0.130.05%   AN       100     3200  10    490   0.250.10%   SA       200     1600  20    980   0.710.10%   ECOLI    100     1600  20    980   0.810.10%   PSA      1200    1600  20    980   0.630.10%   PC       1800    1250  20    980   0.800.10%   CAN      100     15000 20    980   0.270.10%   AN       100     3200  20    980   0.510.20%   SA       200     1600  40    1960  1.430.20%   ECOLI    100     1600  40    1960  1.630.20%   PSA      1200    1600  40    1960  1.260.20%   PC       1800    1250  40    1960  1.590.20%   CAN      100     15000 40    1960  0.530.20%   AN       100     3200  40    1960  1.010.40%   SA       200     1600  80    3920  2.850.40%   ECOLI    100     1600  80    3920  3.250.40%   PSA      1200    1600  80    3920  2.520.40%   PC       1800    1250  80    3920  3.180.40%   CAN      100     15000 80    3920  1.060.40%   AN       100     3200  80    3920  2.030.50%   SA       200     1600  100   4900  3.560.50%   ECOLI    100     1600  100   4900  4.060.50%   PSA      1200    1600  100   4900  3.150.50%   PC       1800    1250  100   4990  3.980.50%   CAN      100     15000 100   4900  1.330.50%   AN       100     3200  100   4900  2.53______________________________________ 
    
     
                       TABLE 7______________________________________20:1 Wt. Ratio GERMALL ® II/IPBCUse Level   Organism Q.sub.a Q.sub.b                          Q.sub.A                               Q.sub.B                                      SI______________________________________0.01%   SA       200     1600   5    95    0.080.01%   ECOLI    100     1600   5    95    0.110.01%   PSA      1200    1600   5    95    0.060.01%   PC       1800    1250   5    95    0.080.01%   CAN      100     15000  5    95    0.060.01%   AN       100     3200   5    95    0.08 0.025% SA       200     1600    12.5                                 237.5                                      0.21 0.025% ECOLI    100     1600    12.5                                 237.5                                      0.27 0.025% PSA      1200    1600    12.5                                 237.5                                      0.16 0.025% PC       1800    1250    12.5                                 237.5                                      0.20 0.025% CAN      100     15000   12.5                                 237.5                                      0.14 0.025% AN       100     3200    12.5                                 237.5                                      0.200.05%   SA       200     1600   25   475   0.420.05%   ECOLI    100     1600   25   475   0.550.05%   PSA      1200    1600   25   475   0.320.05%   PC       1800    1250   25   475   0.390.05%   CAN      100     15000  25   475   0.280.05%   AN       100     3200   25   475   0.400.10%   SA       200     1600   50   950   0.840.10%   ECOLI    100     1600   50   950   1.090.10%   PSA      1200    1600   50   950   0.640.10%   PC       1800    1250   50   950   0.790.10%   CAN      100     15000  50   950   0.560.10%   AN       100     3200   50   950   0.800.20%   SA       200     1600  100  1900   1.690.20%   ECOLI    100     1600  100  1900   2.190.20%   PSA      1200    1600  100  1900   1.270.20%   PC       1800    1250  100  1900   1.580.20%   CAN      100     15000 100  1900   1.130.20%   AN       100     3200  100  1900   1.590.40%   SA       200     1600  200  4800   4.000.40%   ECOLI    100     1600  200  4800   5.000.40%   PSA      1200    1600  200  4800   3.170.40%   PC       1800    1250  200  4800   3.950.40%   CAN      100     15000 200  4800   2.320.40%   AN       100     3200  200  4800   3.500.50%   SA       200     1600  250  4750   4.220.50%   ECOLI    100     1600  250  4750   5.470.50%   PSA      1200    1600  250  4750   3.180.50%   PC       1800    1250  250  4750   3.940.50%   CAN      100     15000 250  4750   2.820.50%   AN       100     3200  250  4750   3.98______________________________________ 
    
     
                       TABLE 8______________________________________2000:1 Wt. Ratio DMDMH/IPBCUse Level   Organism Q.sub.a Q.sub.b                          Q.sub.A                                Q.sub.B                                      SI______________________________________0.01%   SA       200     1600  0.05   99.95                                      0.060.01%   ECOLI    100      800  0.05   99.95                                      0.130.01%   PSA      1200    1600  0.05   99.95                                      0.060.01%   PC       1800    1600  0.05   99.95                                      0.060.01%   CAN      100     16000 0.05   99.95                                      0.010.01%   AN       100     16000 0.05   99.95                                      0.01 0.025% SA       200     1600   0.125                                249.88                                      0.16 0.025% ECOLI    100      800   0.125                                249.88                                      0.31 0.025% PSA      1200    1600   0.125                                249.88                                      0.16 0.025% PC       1800    1600   0.125                                249.88                                      0.16 0.025% CAN      100     16000  0.125                                249.88                                      0.02 0.025% AN       100     16000  0.125                                249.88                                      0.020.05%   SA       200     1600  0.25  499.75                                      0.310.05%   ECOLI    100      800  0.25  499.75                                      0.630.05%   PSA      1200    1600  0.25  499.75                                      0.310.05%   PC       1800    1600  0.25  499.75                                      0.310.05%   CAN      100     16000 0.25  499.75                                      0.030.05%   AN       100     16000 0.25  499.75                                      0.030.10%   SA       200     1600  0.5   999.5 0.630.10%   ECOLI    100      800  0.5   999.5 1.250.10%   PSA      1200    1600  0.5   999.5 0.630.10%   PC       1800    1600  0.5   999.5 0.620.10%   CAN      100     16000 0.5   999.5 0.070.10%   AN       100     16000 0.5   999.5 0.070.20%   SA       200     1600  1     1999  1.250.20%   ECOLI    100      800  1     1999  2.510.20%   PSA      1200    1600  1     1999  1.250.20%   PC       1800    1600  1     1999  1.250.20%   CAN      100     16000 1     1999  0.130.20%   AN       100     16000 1     1999  0.130.40%   SA       200     1600  2     3998  2.510.40%   ECOLI    100      800  2     3998  5.020.40%   PSA      1200    1600  2     3998  2.500.40%   PC       1800    1600  2     3998  2.500.40%   CAN      100     16000 2     3998  0.270.40%   AN       100     16000 2     3998  0.270.50%   SA       200     1600  2.5   4997.5                                      3.140.50%   ECOLI    100      800  2.5   4997.5                                      6.270.50%   PSA      1200    1600  2.5   4997.5                                      3.130.50%   PC       1800    1600  2.5   4997.5                                      3.120.50%   CAN      100     16000 2.5   4997.5                                      0.340.50%   AN       100     16000 2.5   4997.5                                      0.34______________________________________ 
    
     
                       TABLE 9______________________________________1000:1 Wt. Ratio DMDMH/IPBCUse Level   Organism Q.sub.a Q.sub.b                          Q.sub.A                                Q.sub.B                                       SI______________________________________0.01%   SA       200     1600    0.1    99.9                                       0.060.01%   ECOLI    100      800    0.1    99.9                                       0.130.01%   PSA      1200    1600    0.1    99.9                                       0.060.01%   PC       1800    1600    0.1    99.9                                       0.060.01%   CAN      100     16000   0.1    99.9                                       0.010.01%   AN       100     16000   0.1    99.9                                       0.01 0.025% SA       200     1600    0.25                                  249.8                                       0.16 0.025% ECOLI    100      800    0.25                                  249.8                                       0.31 0.025% PSA      1200    1600    0.25                                  249.8                                       0.16 0.025% PC       1800    1600    0.25                                  249.8                                       0.16 0.025% CAN      100     16000   0.25                                  249.8                                       0.02 0.025% AN       100     16000   0.25                                  249.8                                       0.020.05%   SA       200     1600    0.5   499.5                                       0.310.05%   ECOLI    100      800    0.5   499.5                                       0.630.05%   PSA      1200    1600    0.5   499.5                                       0.310.05%   PC       1800    1600    0.5   499.5                                       0.310.05%   CAN      100     16000   0.5   499.5                                       0.040.05%   AN       100     16000   0.5   499.5                                       0.040.10%   SA       200     1600  1      999   0.630.10%   ECOLI    100      800  1      999   1.260.10%   PSA      1200    1600  1      999   0.630.10%   PC       1800    1600  1      999   0.620.10%   CAN      100     16000 1      999   0.070.10%   AN       100     16000 1      999   0.070.20%   SA       200     1600  2     1998   1.260.20%   ECOLI    100      800  2     1998   2.520.20%   PSA      1200    1600  2     1998   1.250.20%   PC       1800    1600  2     1998   1.250.20%   CAN      100     16000 2     1998   0.140.20%   AN       100     16000 2     1998   0.140.40%   SA       200     1600  4     3996   2.520.40%   ECOLI    100      800  4     3996   5.040.40%   PSA      1200    1600  4     3996   2.500.40%   PC       1800    1600  4     3996   2.500.40%   CAN      100     16000 4     3996   0.290.40%   AN       100     16000 4     3996   0.290.50%   SA       200     1600  5     4995   3.150.50%   ECOLI    100      800  5     4995   6.290.50%   PSA      1200    1600  5     4995   3.130.50%   PC       1800    1600  5     4995   3.120.50%   CAN      100     16000 5     4995   0.360.50%   AN       100     16000   2.5   4997.5                                       0.36______________________________________ 
    
     
                       TABLE 10______________________________________500:1 Wt. Ratio DMDMH/IPBCUse Level   Organism Q.sub.a Q.sub.b                          Q.sub.A                               Q.sub.B                                      SI______________________________________0.01%   SA       200     1600    0.2                                  99.8                                      0.060.01%   ECOLI    100      800    0.2                                  99.8                                      0.130.01%   PSA      1200    1600    0.2                                  99.8                                      0.060.01%   PC       1800    1600    0.2                                  99.8                                      0.060.01%   CAN      100     16000   0.2                                  99.8                                      0.010.01%   AN       100     16000   0.2                                  99.8                                      0.01 0.25%  SA       200     1600    0.5                                 249.5                                      0.16 0.25%  ECOLI    100      800    0.5                                 249.5                                      0.32 0.25%  PSA      1200    1600    0.5                                 249.5                                      0.16 0.25%  PC       1800    1600    0.5                                 249.5                                      0.16 0.25%  CAN      100     16000   0.5                                 249.5                                      0.02 0.25%  AN       100     16000   0.5                                 249.5                                      0.020.05%   SA       200     1600  1     499   0.320.05%   ECOLI    100      800  1     499   0.630.05%   PSA      1200    1600  1     499   0.310.05%   PC       1800    1600  1     499   0.310.05%   CAN      100     16000 1     499   0.040.05%   AN       100     16000 1     499   0.040.10%   SA       200     1600  2     998   0.630.10%   ECOLI    100      800  2     998   1.270.10%   PSA      1200    1600  2     998   0.630.10%   PC       1800    1600  2     998   0.620.10%   CAN      100     16000 2     998   0.080.10%   AN       100     16000 2     998   0.080.20%   SA       200     1600  4    1996   1.270.20%   ECOLI    100      800  4    1996   2.540.20%   PSA      1200    1600  4    1996   1.250.20%   PC       1800    1600  4    1996   1.250.20%   CAN      100     16000 4    1996   0.160.20%   AN       100     16000 4    1996   0.160.40%   SA       200     1600  8    3992   2.540.40%   ECOLI    100      800  8    3992   5.070.40%   PSA      1200    1600  8    3992   2.500.40%   PC       1800    1600  8    3992   2.500.40%   CAN      100     16000 8    3992   0.330.40%   AN       100     16000 8    3992   0.330.50%   SA       200     1600  10   4900   3.110.50%   ECOLI    100      800  10   4900   6.230.50%   PSA      1200    1600  10   4900   3.070.50%   PC       1800    1600  10   4900   3.070.50%   CAN      100     16000 10   4900   0.410.50%   AN       100     16000 10   4900   0.41______________________________________ 
    
     
                       TABLE 11______________________________________200:1 Wt. Ratio DMDMH/IPBCUse Level   Organism Q.sub.a Q.sub.b                          Q.sub.A                                 Q.sub.B                                       SI______________________________________0.01%   SA       200     1600    0.5   99.5 0.060.01%   ECOLI    100      800    0.5   99.5 0.130.01%   PSA      1200    1600    0.5   99.5 0.060.01%   PC       1800    1600    0.5   99.5 0.060.01%   CAN      100     16000   0.5   99.5 0.010.01%   AN       100     16000   0.5   99.5 0.01 0.025% SA       200     1600     1.25                                  248.75                                       0.16 0.025% ECOLI    100      800     1.25                                  248.75                                       0.32 0.025% PSA      1200    1600     1.25                                  248.75                                       0.16 0.025% PC       1800    1600     1.25                                  248.75                                       0.16 0.025% CAN      100     16000    1.25                                  248.75                                       0.03 0.025% AN       100     16000    1.25                                  248.75                                       0.030.05%   SA       200     1600    2.5  497.5 0.320.05%   ECOLI    100      800    2.5  497.5 0.650.05%   PSA      1200    1600    2.5  497.5 0.310.05%   PC       1800    1600    2.5  497.5 0.310.05%   CAN      100     16000   2.5  497.5 0.060.05%   AN       100     16000   2.5  497.5 0.060.10%   SA       200     1600   5     995   0.650.10%   ECOLI    100      800   5     995   1.290.10%   PSA      1200    1600   5     995   0.630.10%   PC       1800    1600   5     995   0.620.10%   CAN      100     16000  5     995   0.110.10%   AN       100     16000  5     995   0.110.20%   SA       200     1600  10     1990  1.290.20%   ECOLI    100      800  10     1990  2.590.20%   PSA      1200    1600  10     1990  1.250.20%   PC       1800    1600  10     1990  1.250.20%   CAN      100     16000 10     1990  0.220.20%   AN       100     16000 10     1990  0.220.40%   SA       200     1600  20     3980  2.590.40%   ECOLI    100      800  20     3980  5.180.40%   PSA      1200    1600  20     3980  2.500.40%   PC       1800    1600  20     3980  2.500.40%   CAN      100     16000 20     3980  0.450.40%   AN       100     16000 20     3980  0.450.50%   SA       200     1600  25     4975  3.230.50%   ECOLI    100      800  25     4975  6.470.50%   PSA      1200    1600  25     4975  3.130.50%   PC       1800    1600  25     4975  3.120.50%   CAN      100     16000 25     4975  0.560.50%   AN       100     16000 25     4975  0.56______________________________________ 
    
     
                       TABLE 12______________________________________100:1 Wt. Ratio DMDMH/IPBCUse Level   Organism Q.sub.a Q.sub.b                          Q.sub.A                                Q.sub.B                                      SI______________________________________0.01%   SA       200     1600   1     99   0.070.01%   ECOLI    100      800   1     99   0.130.01%   PSA      1200    1600   1     99   0.060.01%   PC       1800    1600   1     99   0.060.01%   CAN      100     16000  1     99   0.020.01%   AN       100     16000  1     99   0.02 0.025% SA       200     1600    2.5 248   0.17 0.025% ECOLI    100      800    2.5 248   0.33 0.025% PSA      1200    1600    2.5 248   0.16 0.025% PC       1800    1600    2.5 248   0.16 0.025% CAN      100     16000   2.5 248   0.04 0.025% AN       100     16000   2.5 248   0.040.05%   SA       200     1600   5    495   0.330.05%   ECOLI    100      800   5    495   0.670.05%   PSA      1200    1600   5    495   0.310.05%   PC       1800    1600   5    495   0.310.05%   CAN      100     16000  5    495   0.080.05%   AN       100     16000  5    495   0.080.10%   SA       200     1600  10    990   0.670.10%   ECOLI    100      800  10    990   1.340.10%   PSA      1200    1600  10    990   0.630.10%   PC       1800    1600  10    990   0.620.10%   CAN      100     16000 10    990   0.160.10%   AN       100     16000 10    990   0.160.20%   SA       200     1600  20    1980  1.340.20%   ECOLI    100      800  20    1980  2.680.20%   PSA      1200    1600  20    1980  1.250.20%   PC       1800    1600  20    1980  1.250.20%   CAN      100     16000 20    1980  0.320.20%   AN       100     16000 20    1980  0.320.40%   SA       200     1600  40    3960  2.680.40%   ECOLI    100      800  40    3960  5.350.40%   PSA      1200    1600  40    3960  2.510.40%   PC       1800    1600  40    3960  2.500.40%   CAN      100     16000 40    3960  0.650.40%   AN       100     16000 40    3960  0.650.50%   SA       200     1600  50    4950  3.340.50%   ECOLI    100      800  50    4950  6.690.50%   PSA      1200    1600  50    4950  3.140.50%   PC       1800    1600  50    4950  3.120.50%   CAN      100     16000 50    4950  0.810.50%   AN       100     16000 50    4950  0.81______________________________________ 
    
     
                       TABLE 13______________________________________50:1 Wt. Ratio DMDMH/IPBCUse Level   Organism Q.sub.a Q.sub.b                          Q.sub.A                                Q.sub.B                                      SI______________________________________0.01%   SA       200     1600   2     98   0.070.01%   ECOLI    100      800   2     98   0.140.01%   PSA      1200    1600   2     98   0.060.01%   PC       1800    1600   2     98   0.060.01%   CAN      100     16000  2     98   0.030.01%   AN       100     16000  2     98   0.03 0.025% SA       200     1600   5    245   0.18 0.025% ECOLI    100      800   5    245   0.36 0.025% PSA      1200    1600   5    245   0.16 0.025% PC       1800    1600   5    245   0.16 0.025% CAN      100     16000  5    245   0.07 0.025% AN       100     16000  5    245   0.070.05%   SA       200     1600  10    490   0.360.05%   ECOLI    100      800  10    490   0.710.05%   PSA      1200    1600  10    490   0.310.05%   PC       1800    1600  10    490   0.310.05%   CAN      100     16000 10    490   0.130.05%   AN       100     16000 10    490   0.130.10%   SA       200     1600  20    980   0.710.10%   ECOLI    100      800  20    980   1.430.10%   PSA      1200    1600  20    980   0.630.10%   PC       1800    1600  20    980   0.620.10%   CAN      100     16000 20    980   0.260.10%   AN       100     16000 20    980   0.260.20%   SA       200     1600  40    1960  1.430.20%   ECOLI    100      800  40    1960  2.850.20%   PSA      1200    1600  40    1960  1.260.20%   PC       1800    1600  40    1960  1.250.20%   CAN      100     16000 40    1960  0.520.20%   AN       100     16000 40    1960  0.520.40%   SA       200     1600  80    3920  2.850.40%   ECOLI    100      800  80    3920  5.700.40%   PSA      1200    1600  80    3920  2.520.40%   PC       1800    1600  80    3920  2.490.40%   CAN      100     16000 80    3920  1.050.40%   AN       100     16000 80    3920  1.050.50%   SA       200     1600  100   4900  3.560.50%   ECOLI    100      800  100   4900  7.130.50%   PSA      1200    1600  100   4900  3.150.50%   PC       1800    1600  100   4900  3.120.50%   CAN      100     16000 100   4900  1.310.50%   AN       100     16000 100   4900  1.31______________________________________ 
    
     
                       TABLE 14______________________________________20:1 Wt. Ratio DMDMH/IPBCUse Level   Organism Q.sub.a Q.sub.b                          Q.sub.A                                Q.sub.B                                       SI______________________________________0.01%   SA       200     1600   5     95    0.080.01%   ECOLI    100      800   5     95    0.170.01%   PSA      1200    1600   5     95    0.060.01%   PC       1800    1600   5     95    0.060.01%   CAN      100     16000  5     95    0.060.01%   AN       100     16000  5     95    0.06 0.025% SA       200     1600    12.5                                  237.5                                       0.21 0.025% ECOLI    100      800    12.5                                  237.5                                       0.42 0.025% PSA      1200    1600    12.5                                  237.5                                       0.16 0.025% PC       1800    1600    12.5                                  237.5                                       0.16 0.025% CAN      100     16000   12.5                                  237.5                                       0.14 0.025% AN       100     16000   12.5                                  237.5                                       0.140.05%   SA       200     1600   25    475   0.420.05%   ECOLI    100      800   25    475   0.840.05%   PSA      1200    1600   25    475   0.320.05%   PC       1800    1600   25    475   0.310.05%   CAN      100     16000  25    475   0.280.05%   AN       100     16000  25    475   0.280.10%   SA       200     1600   50    950   0.840.10%   ECOLI    100      800   50    950   1.690.10%   PSA      1200    1600   50    950   0.640.10%   PC       1800    1600   50    950   0.620.10%   CAN      100     16000  50    950   0.560.10%   AN       100     16000  50    950   0.560.20%   SA       200     1600  100   1900   1.690.20%   ECOLI    100      800  100   1900   3.380.20%   PSA      1200    1600  100   1900   1.270.20%   PC       1800    1600  100   1900   1.240.20%   CAN      100     16000 100   1900   1.120.20%   AN       100     16000 100   1900   1.120.40%   SA       200     1600  200   4800   4.000.40%   ECOLI    100      800  200   4800   8.000.40%   PSA      1200    1600  200   4800   3.170.40%   PC       1800    1600  200   4800   3.110.40%   CAN      100     16000 200   4800   2.300.40%   AN       100     16000 200   4800   2.300.50%   SA       200     1600  250   4750   4.220.50%   ECOLI    100      800  250   4750   8.440.50%   PSA      1200    1600  250   4750   3.180.50%   PC       1800    1600  250   4750   3.110.50%   CAN      100     16000 250   4750   2.800.50%   AN       100     16000 250   4750   2.80______________________________________ 
    
     Similar SI results also were found with GADM and SUTTOCIDE® A as the methylol compound in place of Germall® II or DMDMH in admixtures with IPBC over the same wt. ratios and use level ranges as shown in the Tables 1-14 above. 
     Tables 1 through 14 above illustrate the synergism of IPBC (compound B) with Germall® II or DMDMH (compound A) at weight ratios of A:B of 2000:1, 1000:1, 500:1, 200:1, 100:1, 50:1 and 20:1. Synergism is very effective for all ratios at low use levels, e.g. 0.01% to 0.1%, against all tested gram-positive, gram-negative organisms and fungi organisms. At slightly higher use concentrations, e.g. 0.20 to 0.50%, all tested ratios were synergistic against Candida albicans and A. niger also. However, at 50:1 and 20:1 ratios, the synergistic effect is negligible at the 0.01 to 0.1% use levels, and non-synergistic even against Candida albicans and A. niger at use levels of 0.20 to 0.50%. 
     The SI values were lower for Germall® II as the methylol compound in the admixtures as compared to DMDMH. 
     Similar results were obtained when iodopropynyl alcohol (IPGA) was substituted for IPBC in the admixtures described above. 
     3. PRESERVATIVE ACTIVITY (CHALLENGE TEST) 
     A typical cosmetic emulsion was prepared for microbiological Challenge testing and predetermined admixtures of a methylol compound and IPBC were added at various use levels. The emulsion thus prepared had the following composition: 
     
         ______________________________________               % wt.______________________________________Phase AStearic Acid          5.00Mineral Oil           2.50Cetyl Alcohol         1.00Lareth-5 and Ceteth-5 and                 0.50Oleth-5 and Steareth-5Glycerol Monostearate and                 1.50Polyoxyethylene StearatePhase BDeionized Water       88.0Triethanolamine 99%   1.00Citric Acid 30% aqueous solution                 0.60Preservative Admixture                 qs______________________________________ 
    
     To prepare the emulsion, Phases A and B were heated separately to 75°-80° C. Phase A then was added to Phase B with mixing. The mixture then was cooled to 55°-60° C. At this point the desired amount of the preservative admixture was added and the product was cooled to 50° C. while stirring. The citric acid solution then was added to adjust the pH and the mixture was stirred until a temperature of 30° C. was reached. 
     The challenge tests were carried out using the following microorganisms: SA, ECOLI, PSA, PC, AN and CAN, in this manner. 50 g. aliquots of the test emulsion containing various amounts of the preservative admixture were inoculated with approximately 10 7  -10 8  of the challenge organisms. The test samples then were stirred to disperse the challenge inoculum. The samples were incubated and assayed at 48 hours, 7, 14, 21 and 28 days. The assays were performed on 1 g. of the test sample by serially diluting 10 1  to 10 6  of the original concentration. The plating medium for bacteria was Letheen agar and for fungi it was low pH Mycophil agar with Tween 20. Each plated sample was incubated for 48 hours at 37° C. for bacteria, 5 days at 25° C. for mold, and 3 days at 25° C. for fungi. After incubation, readings of the number of colonies per milliliter (cfu/ml) were made. At 21 days the test product was reinoculated with half of the original inoculum. The data is presented in Tables 15-23 below. 
     
                                           TABLE 15__________________________________________________________________________2000:1 GERMALL ® II/IPBC__________________________________________________________________________Test Level Organism       48 Hours            7 Days                 14 Days                      21 Days                            28 Days__________________________________________________________________________0.01% AN    69,000            260,000                 190,000                      17,000                            4,5000.01% CAN   98,000            76,000                 1,400                      3,100 19,0000.01% ECOLI 110,000            290,000                 2,400                      138,000                            560,0000.01% PC    &lt;10  &lt;10  &lt;10  &lt;10   &lt;100.01% PSA   &lt;10  &lt;10  &lt;10  &lt;10   &lt;100.01% SA    190,000            220  &lt;10  &lt;10   3,700 0.025% AN    2,800            10   10   &lt;10   220 0.025% CAN   58,000            29,000                 18,000                      56,000                            110,000 0.025% ECOLI 39,000            10   &lt;10  &lt;10   &lt;10 0.025% PC    &lt;10  &lt;10  &lt;10  &lt;10   &lt;10 0.025% PSA   &lt;10  &lt;10  &lt;10  &lt;10   &lt;10 0.025% SA    37,000            170  &lt;10  &lt;10   &lt;100.05% AN    20   &lt;10  &lt;10  &lt;10   &lt;100.05% CAN   19,000            6,600                 70   &lt;10   3200.05% ECOLI 3,400            &lt;10  &lt;10  &lt;10   &lt;100.05% PC    &lt;10  &lt;10  &lt;10  &lt;10   &lt;100.05% PSA   &lt;10  &lt;10  &lt;10  &lt;10   &lt;100.05% SA    31,000            &lt;10  &lt;10  &lt;10   &lt;100.1%  AN    &lt;10  &lt;10  &lt;10  &lt;10   &lt;100.1%  CAN   180  &lt;10  &lt;10  &lt;10   &lt;100.1%  ECOLI &lt;10  &lt;10  &lt;10  &lt;10   &lt;100.1%  PC    &lt;10  &lt;10  &lt;10  &lt;10   &lt;100.1%  PSA   &lt;10  &lt;10  &lt;10  &lt;10   &lt;100.1%  SA    750  &lt;10  &lt;10  &lt;10   &lt;100.2%  AN    &lt;10  &lt;10  &lt;10  &lt;10   &lt;100.2%  CAN   &lt;10  &lt;10  &lt;10  &lt;10   &lt;100.2%  ECOLI &lt;10  &lt;10  &lt;10  &lt;10   &lt;100.2%  PC    &lt;10  &lt;10  &lt;10  &lt;10   &lt;100.2%  PSA   &lt;10  &lt;10  &lt;10  &lt;10   &lt;100.2%  SA    &lt;10  &lt;10  &lt;10  &lt;10   &lt;10Unpreserved controlAN          52,000            27,000                 19,000                      19,000                            19,000CAN         110,000            130,000                 240,000                      180,000                            240,000ECOLI       54,000            140,000                 170,000                      170,000                            74,000PC          6,400,000            6,400,000                 2,000,000                      6,700,000                            29,000PSA         110,000            700  110,000                      290,000                            85,000SA          2,800,000            250,000                 51,000                      3,700 330Inoculum Concentration       0 Hours            21 DaysAN          26,000            53,000CAN         1,000,000            1,900,000ECOLI       3,600,000            170,000PC          3,400,000            87,000PSA         4,500,000            390,000SA          4,100,000            200,000__________________________________________________________________________ 
    
     
                                           TABLE 16__________________________________________________________________________1000:1 GERMALL ® II/IPBC__________________________________________________________________________Test Level Organism      48 Hours            7 Days                 14 Days                       21 Days                            28 Days__________________________________________________________________________0.01% AN   34,000            3,500                 80    10   &lt;100.01% CAN  420,000            24,000                 950   6,400                            6,400,0000.01% ECOLI      120,000            63,000                 93,000                       92,000                            9,600,0000.01% PC   10    25,000                 1,600 15,800                            33,000,0000.01% PSA  &lt;10   &lt;10  &lt;10   &lt;10  &lt;100.01% SA   100,000            1,400                 &lt;10   &lt;10  5,000 0.025% AN   530   10   &lt;10   &lt;10  &lt;10 0.025% CAN  34,000            750  10    770  240,000 0.025% ECOLI      120,000            &lt;10  &lt;10   &lt;10  &lt;10 0.025% PC   &lt;10   &lt;10  &lt;10   &lt;10  &lt;10 0.025% PSA  &lt;10   &lt;10  &lt;10   &lt;10  &lt;10 0.025% SA   37,000            170  &lt;10   &lt;10  &lt;100.05% AN   &lt;10   &lt;10  &lt;10   &lt;10  &lt;100.05% CAN  13,000            &lt;10  &lt;10   &lt;10  &lt;100.05% ECOLI      68,000            &lt;10  &lt;10   &lt;10  &lt;100.05% PC   &lt;10   &lt;10  &lt;10   &lt;10  &lt;100.05% PSA  &lt;10   &lt; 10 &lt;10   &lt;10  &lt;100.05% SA   21,000            &lt;10  &lt;10   &lt;10  &lt;100.1%  AN   &lt;10   &lt;10  &lt;10   &lt;10  &lt;100.1%  CAN  10    &lt;10  &lt;10   &lt;10  &lt;100.1%  ECOLI      &lt;10   &lt;10  &lt;10   &lt;10  &lt;100.1%  PC   &lt;10   &lt;10  &lt;10   &lt;10  &lt;100.1%  PSA  &lt;10   &lt;10  &lt;10   &lt;10  &lt;100.1%  SA   1,400 &lt;10  &lt;10   &lt;10  &lt;100.2%  AN   &lt;10   &lt;10  &lt;10   &lt;10  &lt;100.2%  CAN  &lt;10   &lt;10  &lt;10   &lt;10  &lt;100.2%  ECOLI      &lt;10   &lt;10  &lt;10   &lt;10  &lt;100.2%  PC   &lt;10   &lt;10  &lt;10   &lt;10  &lt;100.2%  PSA  &lt;10   &lt;10  &lt;10   &lt;10  &lt;100.2%  SA   &lt;10   &lt;10  &lt;10   &lt;10  &lt;10Unpreserved controlAN         52,000            27,000                 19,000                       19,000                            19,000CAN        110,000            130,000                 240,000                       180,000                            240,000ECOLI      54,000            140,000                 170,000                       170,000                            74,000PC         6,400,000            6,400,000                 2,000,000                       6,700,000                            29,000PSA        110,000            700  110,000                       290,000                            85,000SA         2,800,000            250,000                 51,000                       3,700                            330Inoculum Concentration      0 Hours            21 DaysAN         26,000            53,000CAN        1,000,000            1,900,000ECOLI      3,600,000            170,000PC         3,400,000            87,000PSA        4,500,000            390,000SA         4,100,000            200,000__________________________________________________________________________ 
    
     
                                           TABLE 17__________________________________________________________________________500:1 GERMALL ® II/IPBC__________________________________________________________________________Test Level Organism      48 Hours            7 Days                  14 Days                       21 Days                            28 Days__________________________________________________________________________0.01% AN   23,000            40    &lt;10  &lt;10  &lt;100.01% CAN  170,000            5,600 290  200  &lt;100.01% ECOLI      90,000            57,000                  95,000                       70,000                            240,0000.01% PC   10    &lt;10   &lt;10  &gt;10,000                            42,000,0000.01% PSA  &lt;10   &lt;10   &lt;10  &lt;10  &lt;100.01% SA   380,000            440   &lt;10  &lt;10  5,1000.05% AN   &lt;10   &lt;10   &lt;10  &lt;10  &lt;100.05% CAN  8,700 &lt;10   &lt;10  &lt;10  &lt;100.05% ECOLI      60,000            &lt;10   &lt;10  &lt;10  &lt;100.05% PC   &lt;10   &lt;10   &lt;10  &lt;10  &lt;100.05% PSA  &lt;10   &lt;10   &lt;10  &lt;10  &lt;100.05% SA   31,000            &lt;10   &lt;10  &lt;10  &lt;100.1%  AN   &lt;10   &lt;10   &lt;10  &lt;10  &lt;100.1%  CAN  &lt;10   &lt;10   &lt;10  &lt;10  &lt;100.1%  ECOLI      &lt;10   &lt;10   &lt;10  &lt;10  &lt;100.1%  PC   &lt;10   &lt;10   &lt;10  &lt;10  &lt;100.1%  PSA  &lt;10   &lt;10   &lt;10  &lt;10  &lt;100.1%  SA   890   &lt;10   &lt;10  &lt;10  &lt;100.2%  AN   &lt;10   &lt;10   &lt;10  &lt;10  &lt;100.2%  CAN  &lt;10   &lt;10   &lt;10  &lt;10  &lt;100.2%  ECOLI      &lt;10   &lt;10   &lt;10  &lt;10  &lt;100.2%  PC   &lt;10   &lt;10   &lt;10  &lt;10  &lt;100.2%  PSA  &lt;10   &lt;10   &lt;10  &lt;10  &lt;100.2%  SA   &lt;10   &lt;10   &lt;10  &lt;10  &lt;10Unpreserved controlAN         6,100 520,000                  18,000                       5,000                            11,000CAN        1,000,000            710,000                  95,000                       12,000                            64,000ECOLI      7,100,000            6,200,000                  610,000                       350,000                            120,000PC         14,600,000            160,000,000                  3,600,000                       2,720,000                            9,500,000PSA        20    900   130  4,100                            &gt;100,000SA         43,000,000            600,000                  1,000                       220  &lt;10Inoculum Concentration      0 Hours            21 DaysAN         19,000            4,700,000CAN        340,000            16,000,000ECOLI      3,900,000            1,480,000PC         3,800,000            1,380,000PSA        9,200,000            730,000SA         4,800,000            360,000__________________________________________________________________________ 
    
     
                                           TABLE 18__________________________________________________________________________200:1 GERMALL ® II/IPBC__________________________________________________________________________Test Level Organism      48 Hours            7 Days                 14 Days                       21 Days                            28 Days__________________________________________________________________________0.01% AN   47,000            320  10    &lt;10  &lt;100.01% CAN  810,000            450,000                 410,000                       190,000                            63,0000.01% ECOLI      220,000            7,600                 &lt;10   850  &gt;1,000,0000.01% PC   10,000            500,000                 1,900,000                       1,100,000                            193,0000.01% PSA  &lt;10   &lt;10  &lt;10   &lt;10  &lt;100.01% SA   190,000            23,000                 120   &lt;10  &lt;100.05% AN   &lt;10   &lt;10  &lt;10   &lt;10  &lt;100.05% CAN  190   &lt;10  &lt;10   &lt;10  &lt;100.05% ECOLI      37,000            &lt;10  &lt;10   &lt;10  &lt;100.05% PC   &lt;10   &lt;10  &lt;10   &lt;10  &lt;100.05% PSA  &lt;10   &lt;10  &lt;10   &lt;10  &lt;100.05% SA   19,000            &lt;10  &lt;10   &lt;10  &lt;100.1%  AN   &lt;10   &lt;10  &lt;10   &lt;10  &lt;100.1%  CAN  10    &lt;10  &lt;10   &lt;10  &lt;100.1%  ECOLI      &lt;10   &lt;10  &lt;10   &lt; 10 &lt;100.1%  PC   &lt;10   &lt;10  &lt;10   &lt;10  &lt;100.1%  PSA  &lt;10   &lt;10  &lt;10   &lt;10  &lt;100.1%  SA   45,000            &lt;10  &lt;10   &lt;10  &lt;100.2%  AN   &lt;10   &lt;10  &lt;10   &lt;10  &lt;100.2%  CAN  &lt;10   &lt;10  &lt;10   &lt;10  &lt;100.2%  ECOLI      &lt;10   &lt;10  &lt;10   &lt;10  &lt;100.2%  PC   &lt;10   &lt;10  &lt;10   &lt;10  &lt;100.2%  PSA  &lt;10   &lt;10  &lt;10   &lt;10  &lt;100.2%  SA   &lt;10   &lt;10  &lt;10   &lt;10  &lt;10Unpreserved controlAN         89,000            32,000                 22,000                       16,000                            16,000CAN        210,000            670,000                 430,000                       590,000                            640,000ECOLI      640,000            360,000                 410,000                       990,000                            68,000PC         19,000,000            3,200,000                 7,000,000                       &gt;10,000                            2,760,000PSA        80    9,400                 200,000                       &gt;10,000                            34,000SA         6,300,000            190,000                 11,000                       580  120Inoculum Concentration      0 Hours            21 DaysAN         41,000            32,000CAN        640   1,100,000ECOLI      5,800,000            1,300,000PC         900,000            3,000,000PSA        1,800,000            4,900,000SA         7,200,000            2,000,000__________________________________________________________________________ 
    
     
                                           TABLE 19__________________________________________________________________________50:1 GERMALL ® II/IPBC__________________________________________________________________________Test Level Organism      48 Hours            7 Days                 14 Days                       21 Days                            28 Days__________________________________________________________________________0.01% AN   4,100 40   &lt;10   &lt;10  &lt;100.01% CAN  310,000            7,700                 560   5,600                            5,2000.01% ECOLI      170,000            710  10    &lt;10  1200.01% PC   7,400 74,000                 340,000                       720,000                            520,0000.01% PSA  &lt;10   &lt;10  &lt;10   &lt;10  300.01% SA   110,000            11,000                 &lt;10   &lt;10  9,2000.05% AN   &lt;10   100  &lt;10   &lt;10  &lt;100.05% CAN  210   &lt;10  &lt;10   &lt;10  &lt;100.05% ECOLI      150,000            &lt;10  &lt;10   &lt;10  &lt;100.05% PC   &lt;10   &lt;10  &lt;10   &lt;10  &lt;100.05% PSA  &lt;10   &lt;10  &lt;10   &lt;10  &lt;100.05% SA   35,000            &lt;10  &lt;10   &lt;10  &lt;100.1%  AN   &lt;10   &lt;10  &lt;10   &lt;10  &lt;100.1%  CAN  &lt;10   &lt;10  &lt;10   &lt;10  &lt;100.1%  ECOLI      510   &lt;10  &lt;10   &lt;10  &lt;100.1%  PC   &lt;10   &lt;10  &lt;10   &lt;10  &lt;100.1%  PSA  &lt;10   &lt;10  &lt;10   &lt;10  &lt;100.1%  SA   3,000 &lt;10  &lt;10   &lt;10  &lt;100.2%  AN   &lt;10   &lt;10  &lt;10   &lt;10  &lt;100.2%  CAN  &lt;10   &lt;10  &lt;10   &lt;10  &lt;100.2%  ECOLI      &lt;10   &lt;10  &lt;10   &lt;10  &lt;100.2%  PC   &lt;10   &lt;10  &lt;10   &lt;10  &lt;100.2%  PSA  &lt;10   &lt;10  &lt;10   &lt;10  &lt;100.2%  SA   &lt;10   &lt;10  &lt;10   &lt;10  &lt;10Unpreserved controlAN         89,000            32,000                 22,000                       16,000                            16,000CAN        210,000            670,000                 430,000                       590,000                            640,000ECOLI      640,000            360,000                 410,000                       990,000                            68,000PC         19,000,000            3,200,000                 7,000,000                       &gt;10,000                            2,760,000PSA        80    9,400                 200,000                       &gt;10,000                            34,000SA         6,300,000            190,000                 11,000                       580  120Inoculum Concentration      0 Hours            21 DaysAN         41,000            32,000CAN        640   1,100,000ECOLI      5,800,000            1,300,000PC         900,000            3,000,000PSA        1,800,000            4,900,000SA         7,200,000            2,000,000__________________________________________________________________________ 
    
     
                                           TABLE 20__________________________________________________________________________20:1 GERMALL ® II/IPBC__________________________________________________________________________Test Level Organism      48 Hours            7 Days                 14 Days                      21 Days                            28 Days__________________________________________________________________________0.01% AN   3,100 &lt;10  &lt;10  &lt;10   &lt;100.01% CAN  75,000            220  &lt;10  &lt;10   2,4000.01% ECOLI      160,000            110  &lt;10  &lt;10   200.01% PC   12,000            1,000,000                 2,100,000                      &gt;1,000,000                            730,0000.01% PSA  &lt;10   &lt;10  &lt;10  &lt;10   4,0000.01% SA   140,000            4,100                 &lt;10  &lt;10   1,6800.05% AN   &lt;10   &lt;10  &lt;10  &lt;10   &lt;100.05% CAN  &lt;10   &lt;10  &lt;10  &lt;10   &lt;100.05% ECOLI      16,000            &lt;10  &lt;10  &lt;10   &lt;100.05% PC   &lt;10   &lt;10  &lt;10  &lt;10   &lt;100.05% PSA  &lt;10   &lt;10       &lt;10   &lt;100.05% SA   31,000            &lt;10  &lt;10  &lt;10   &lt;100.1%  AN   &lt;10   &lt;10  &lt;10  &lt;10   &lt;100.1%  CAN  &lt;10   &lt;10  &lt;10  &lt;10   &lt;100.1%  ECOLI      &lt;10   &lt;10  &lt;10  &lt;10   &lt;100.1%  PC   &lt; 10  &lt;10  &lt;10  &lt;10   &lt;100.1%  PSA  &lt;10   &lt;10  &lt;10  &lt;10   &lt;100.1%  SA   6,800 &lt;10  &lt;10  &lt;10   &lt;100.2%  AN   &lt;10   &lt;10  &lt;10  &lt;10   &lt;100.2%  CAN  &lt;10   &lt;10  &lt;10  &lt;10   &lt;100.2%  ECOLI      &lt;10   &lt;10  &lt;10  &lt;10   &lt;100.2%  PC   &lt;10   &lt;10  &lt;10  &lt;10   &lt;100.2%  PSA  &lt;10   &lt;10  &lt;10  &lt;10   &lt;100.2%  SA   &lt;10   &lt;10  &lt;10  &lt;10   &lt;10Unpreserved controlAN         89,000            32,000                 22,000                      16,000                            16,000CAN        210,000            670,000                 430,000                      590,000                            640,000ECOLI      640,000            360,000                 410,000                      990,000                            68,000PC         19,000,000            3,200,000                 7,000,000                      &gt;10,000                            2,760,000PSA        80    9,400                 200,000                      &gt;10,000                            34,000SA         6,300,000            190,000                 11,000                      580   120Inoculum Concentration      0 Hours            21 DaysAN         41,000            32,000CAN        640   1,100,000ECOLI      5,800,000            1,300,000PC         900,000            3,000,000PSA        1,800,000            4,900,000SA         7,200,000            2,000,000__________________________________________________________________________ 
    
     
                                           TABLE 21__________________________________________________________________________2000:1 DMDMH/IPBC__________________________________________________________________________Test Level Organism      48 Hours            7 Days                  14 Days                       21 Days                            28 Days__________________________________________________________________________ 0.025% AN   4,100 38,000                  270  80   100 0.025% CAN  270,000            1,900,000                  550,000                       220,000                            210,000 0.025% ECOLI      1,300,000            &lt;10   &lt;10  &lt;10  3,000 0.025% PC   &lt;10   &lt;10   &lt;10  &lt;10  &lt;10 0.025% PSA  &lt;10   &lt;10   &lt;10  &lt;10  &lt;10 0.025% SA   42,000            20    &lt;10  &lt;10  &lt;100.05% AN   &lt;10   &lt;10   &lt;10  &lt;10  &lt;100.05% CAN  770,000            130,000                  670,000                       160,000                            64,0000.05% ECOLI      220,000            &lt;10   &lt;10  &lt;10  &lt;100.05% PC   &lt;10   &lt;10   &lt;10  &lt;10  &lt;100.05% PSA  &lt;10   &lt;10   &lt;10  &lt;10  &lt;100.05% SA   9,000 &lt;10   &lt;10  &lt;10  &lt;100.1%  AN   &lt;10   &lt;10   &lt;10  &lt;10  &lt;100.1%  CAN  580   840   500  40,000                            83,0000.1%  ECOLI      340   &lt;10   &lt;10  &lt;10  &lt;100.1%  PC   &lt;10   &lt;10   &lt;10  &lt; 10 &lt;100.1%  PSA  &lt;10   &lt;10   &lt;10  &lt;10  &lt;100.1%  SA   29,000            &lt;10   &lt;10  &lt;10  &lt;100.2%  AN   &lt;10   &lt;10   &lt;10  &lt;10  &lt;100.2%  CAN  &lt;10   10    10   &lt;10  210.2%  ECOLI      &lt;10   &lt;10   &lt;10  &lt;10  &lt;100.2%  PC   &lt;10   &lt;10   &lt;10  &lt;10  &lt;100.2%  PSA  &lt;10   &lt;10   &lt;10  &lt;10  &lt;100.2%  SA   &lt;10   &lt;10   &lt;10  &lt;10  &lt;10Unpreserved controlAN         37,000            36,000                  24,000                       5,200                            6,000CAN        120,000            1,900,000                  3,300,000                       480,000                            790,000ECOLI      150,000            2,500,000                  7,300,000                       240,000                            140,000PC         19,000,000            15,600,000                  5,900,000                       8,500,000                            31,000,000PSA        &lt;10   &lt;10   100  15,200                            300,000SA         7,000,000            &gt;1,000,000                  12,000                       3,000                            110Inoculum Concentration      0 Hours            21 DaysAN         50,000            41,000CAN        1,400,000            640ECOLI      4,800,000            5,800,000PC         9,200,000            900,000PSA        6,900,000            9,000,000SA         5,700,000            7,200,000__________________________________________________________________________ 
    
     
                                           TABLE 22__________________________________________________________________________1000:1 DMDMH/IPBC__________________________________________________________________________Test Level Organism      48 Hours            7 Days                  14 Days                       21 Days                            28 Days__________________________________________________________________________ 0.025% AN   650   900   &lt;10  &lt;10  140 0.025% CAN  97,000            900,000                  2,300,000                       120,000                            120,000 0.025% ECOLI      160,000            &lt;10   &lt;10  &lt;10  2,400 0.025% PC   &lt;10   &lt;10   &lt;10  &lt;10  &lt;10 0.025% PSA  &lt;10   &lt;10   &lt;10  &lt;10  &lt;10 0.025% SA   23,000            &lt;10   &lt;10  &lt;10  900.05% AN   20    &lt;10   &lt;10  &lt;10  &lt;100.05% CAN  65,000            520,000                  56,000                       32,000                            370,0000.05% ECOLI      26,000            &lt;10   &lt;10  &lt;10  &lt;100.05% PC   &lt;10   &lt;10   &lt;10  &lt;10  &lt;100.05% PSA  &lt;10   &lt;10   &lt;10  &lt;10  &lt;100.05% SA   12,000            &lt;10   &lt;10  &lt;10  &lt;100.1%  AN   &lt;10   &lt;10   &lt;10  &lt;10  &lt;100.1%  CAN  3,100 4,800 180  640  4,4000.1%  ECOLI      45,000            &lt;10   &lt;10  &lt; 10 &lt;100.1%  PC   &lt;10   &lt;10   &lt;10  &lt;10  &lt;100.1%  PSA  &lt;10   &lt;10   &lt;10  &lt;10  &lt;100.1%  SA   6,000 &lt;10   &lt;10  &lt;10  &lt;100.2%  AN   &lt;10   &lt;10   &lt;10  &lt;10  &lt;100.2%  CAN  &lt;10   &lt;10   &lt;10  &lt;10  &lt;100.2%  ECOLI      &lt;10   &lt;10   &lt;10  &lt;10  &lt;100.2%  PC   &lt;10   &lt;10   &lt;10  &lt;10  &lt;100.2%  PSA  &lt;10   &lt;10   &lt;10  &lt;10  &lt;100.2%  SA   180   &lt;10   &lt;10  &lt;10  &lt;10Unpreserved controlAN         37,000            36,000                  24,000                       5,200                            6,000CAN        120,000            1,900,000                  3,300,000                       480,000                            790,000ECOLI      150,000            2,500,000                  7,300,000                       240,000                            140,000PC         19,000,000            15,600,000                  5,900,000                       8,500,000                            31,000,000PSA        &lt;10   &lt;10   100  15,200                            300,000SA         7,000,000            &gt;1,000,000                  12,000                       3,000                            110Inoculum Concentration      0 Hours            21 DaysAN         50,000            41,000CAN        1,400,000            640ECOLI      4,800,000            5,800,000PC         9,200,000            900,000PSA        6,900,000            9,000,000SA         5,700,000            7,200,000__________________________________________________________________________ 
    
     
                                           TABLE 23__________________________________________________________________________2000:1 GADM/IPBC__________________________________________________________________________Test Level Organism      48 Hours            7 Days                  14 Days                        21 Days                              28 Days__________________________________________________________________________ 0.025% AN   300   21    10    &lt;10   11 0.025% CAN  480,000            890,000                  940,000                        1,040,000                              130,000 0.025% ECOLI      230,000            &lt;10   &lt;10   &lt;10   &lt;10 0.025% PC   &lt;10   &lt;10   &lt;10   &lt;10   &lt;10 0.025% PSA  &lt;10   &lt;10   &lt;10   &lt;10   &lt;10 0.025% SA   78,000            &lt;10   &lt;10   &lt;10   &lt;100.05% AN   &lt;10   &lt;10   &lt;10   &lt;10   &lt;100.05% CAN  110,000            4,100,000                  3,600,000                        330,000                              97,0000.05% ECOLI      120,000            &lt;10   &lt;10   &lt;10   &lt;100.05% PC   &lt;10   &lt;10   &lt;10   &lt;10   &lt;100.05% PSA  &lt;10   &lt;10   &lt;10   &lt;10   &lt;100.05% SA   56,000            &lt;10   &lt;10   &lt;10   &lt;100.1%  AN   &lt;10   &lt;10   &lt;10   &lt;10   &lt;100.1%  CAN  1,200,000            53,000                  430,000                        144,000                              110,0000.1%  ECOLI      &lt;10   &lt;10   &lt;10   &lt;10   &lt;100.1%  PC   &lt;10   &lt; 10  &lt;10   &lt;10   &lt;100.1%  PSA  &lt;10   &lt;10   &lt;10   &lt;10   &lt;100.1%  SA   &lt;10   &lt;10   &lt;10   &lt;10   &lt;100.2%  AN   &lt;10   &lt;10   &lt;10   &lt;10   &lt;100.2%  CAN  90,000            &lt;10   &lt;10   &lt;10   600.2%  ECOLI      &lt;10   &lt;10   &lt;10   &lt;10   &lt;100.2%  PC   &lt;10   &lt;10   &lt;10   &lt;10   &lt;100.2%  PSA  &lt;10   &lt;10   &lt;10   &lt;10   &lt;100.2%  SA   &lt;10   &lt;10   &lt;10   &lt;10   &lt;10Unpreserved controlAN         50,000            33,000                  33,000                        13,000                              5,400CAN        780,000            780,000                  780,000                        500,000                              170,000ECOLI      600,000            3,100,000                  920,000                        920,000                              140,000PC         11,000,000            30,000,000                  10,000,000                        10,000,000                              1,400,000PSA        3,800 600   12,800                        12,800                              100,000SA         14,000,000            410,000                  7,100 7,100 80Inoculum Concentration      0 Hours            21 DaysAN         53,000            10,000CAN        1,900,000            310,000ECOLI      170,000            3,500,000PC         87,000            2,500,000PSA        390,000            5,400,000SA         200,000            4,100,000__________________________________________________________________________ 
    
     Discussion of Challenge Testing Results 
     The 28-day challenge results reported in Tables 15-23 above demonstrate the effectiveness of the preservative admixture of the invention in a use emulsion composition against a wide range of bacteria and fungi organisms. 
     For example, admixture compositions of Germall® II and IPBC at a wt. ratio of 2000:1 (Table 15), when present at use levels of 0.05 to 0.2%, corresponding to 0.75 to 10 ppm IPBC and 500 to 2000 ppm methylol levels, provide substantially complete protection against all tested organisms after 28 days. At the low use level of 0.05% active, all the challenge tests passed within 21 days. Then, upon reinoculation after 21 days, all organisms died within 7 days except CAN which cleared within 14 days. 
     Table 21 shows the challenge test results for DMDMH and IPBC admixtures at the same 2000:1 wt. ratio. A use level of 0.2%, however is needed for this blend to pass against all organisms after 21 days. Upon reinoculation, all organisms died within 7 days with the exception of CAN which cleared within 14 days. It is thus evident from these results that Germall® II blended with IPBC is 4 times more effective than a DMDMH/IPBC blend 
     Germall® II also is superior to GADM as the methylol compound, as shown in Table 23. 
     While the invention has been described with particular reference to certain embodiments thereof, it will be understood that changes and modifications may be made which are within the skill of the art. Accordingly, it is intended to be bound only by the following claims, in which: