Abstract:
A composition is provided comprising  N. meningitidis  outer membrane vesicles, wherein said outer membrane vesicles are enriched with at least one antigenic component. The composition is suitable for use in vaccines and for treatment of infection, particularly meningococcal infection, demonstrating a broad spectrum of protection. A number of preferred antigenic components are described and include antigenic proteins and proteoglycans derived from  N. meningitidis.

Description:
CROSS-REFERENCE TO RELATED APPLICATIONS  
       [0001]    This Application is a Continuation-in-Part of U.S. application Ser. No. 09/830,854 with a 102(e) filing date of Aug. 29, 2001, which is a 371 of International Application No. PCT/GB99/03626 published under PCT Article 21(2) in English, with an International Filing date of Nov. 2, 1999, which claims priority to British Patent Application No. 9823978.3, filed Nov. 2, 1998, the contents of each of these applications are fully incorporated by reference. 
     
    
     
       BACKGROUND OF THE INVENTION  
       Field of the Invention  
         [0002]    The present invention relates to a multicomponent vaccine and methods for preparing a multicomponent vaccine that confers protective immunity to a broad spectrum of infection by Gram negative pathogens. In particular the present invention relates to a multicomponent vaccine that provides both passive and active protective immunity to meningococcal disease.  
           [0003]    Meningococcal meningitis is a major problem worldwide and in many countries incidence of infection is increasing.  Neisseria meningitidis  is the causative agent of the disease and is also responsible for meningococcal septicaemia, which is associated with rapid onset and high mortality, with around 22% of cases proving fatal.  
           [0004]    At present, vaccines directed at providing protective immunity against meningococcal disease provide only limited protection because of the many different strains of  N. meningitidis.  Vaccines based upon the serogroup antigens, the capsular polysaccharides, offer only short lived protection against infection and do not protect against many strains commonly found in North America and Europe. A further drawback of these vaccines is that they provide low levels of protection for children under the age of 2 years, one of the most vulnerable groups that are commonly susceptible to infection.  
           [0005]    The meningococcal transferrin receptor is made up of two types of component protein chain, Transferrin binding protein A (TbpA) and TbpB. The receptor complex is believed to be formed from a dimer of TbpA which associates with a single TbpB (Boulton et al. (1998)). Epitopes present in TbpA are known to be masked within the interior of the protein (Ala′Aldeen (1996)). Vaccines against meningococcal meningitis based on TbpB from one strain alone show some cross reactivity and there is evidence of a cross-reactive immune response in rabbits immunised with TbpB alone (Feirrerós et al. (1998)).  
           [0006]    It would be of advantage, nevertheless, to provide a vaccine that gives a broader range of protective immunity to infection from a wider spectrum of strains of  N. meningitidis.  It would be of further advantage to provide a vaccine that confers protective immunity to infants as well as adults and whose protection is long term. It would also be of advantage to provide a vaccine that protects against sub-clinical infection, i.e. where symptoms of meningococcal infection are not immediately apparent that the infected individual may act as a carrier of the pathogen.  
           [0007]    It is an object of the present invention to provide compositions containing Tbps, and vaccines based thereon, that meet or at least ameliorate the disadvantages in the art. In particular, it is an object of the invention to provide a vaccine composition that consistently and reliably induces protective immunity to meningococcal infection.  
           [0008]    Accordingly, a first aspect of the present invention provides a composition comprising both transferrin binding proteins A (TbpA) and B (TbpB), suitably in a molar ratio of about 2:1 (TbpA:TbpB). In a preferred embodiment of the present invention the molar ratio of TbpA to TbpB is 2:1.  
           [0009]    The composition may be combined with a pharmaceutically acceptable carrier—for example the adjuvant alum although any carrier suitable for oral, intravenous, subcutaneous, intraperitoneal or any other route of administration is suitable—to produce a pharmaceutical composition for treatment of meningococcal disease.  
           [0010]    The present invention thus provides for a vaccine comprising both TbpA+B proteins, preferably with a molar ratio of between 1.8 and 2.2 molecules of TbpA to one molecule of TbpB, more preferably 2 molecules of TbpA to one of TbpB. This particular combination of components, surprisingly, can provide higher protective immunity to meningococcal infection, compared to vaccination with TbpB alone. In a specific embodiment of the invention, described in more detail below, a 1:1 combination of A:B is more protective against challenge than B alone. This is surprising as TbpA has previously been considered to be non-protective. The present results differ from this established view with some experiments (described in more detail below) showing that, when administered as a vaccine, TbpA is also able to provide protective immunity to meningococcal infection. However, the present results most strikingly demonstrate the consistent performance of vaccines that comprise both Tbps A and B compared to those comprising Tbp A or B alone. It is this lack of variability between compositions and the consistently high level of protection to infection induced in response to vaccination with Tbp A+B, that enables the compositions of the invention to demonstrate significant advantage over the vaccines of the prior art.  
           [0011]    Transferrin binding proteins are known to be located on the outer membranes of a number of Gram negative bacteria such as  N. meningitidis.  Formulations of the composition of the present invention with conventional carriers or adjuvants provide a composition for treatment of infection by these bacteria.  
           [0012]    It is an advantage that following administration of a composition according to the present invention antibodies may be raised against epitopes that consist of sequences from TbpA and TbpB in juxtaposition. Thus, the immune response obtainable using such a composition may be improved compared with that from prior art vaccine compositions which comprise only one component of the Tbp complex and in which the full range of potential Tbp epitopes are unavailable. It is a further option in the present invention for one Tbp subunit component of the TbpA+B complex to be from a first strain of  N. meningitidis  and another from a second strain different from the first. For example, the TbpA dimer is taken from the first strain and the TbpB is from the second. The TbpA and TbpB proteins may be selected independently from strains K454, H44/76 and B16B6. In all aspects of the invention the Tbps can be directly isolated from the bacterial source or can be produced by recombinant methods commonly known in the art. Combinations of proteins from other strains are also envisaged, and the combining of components from different strains of bacteria offers the potential for providing an individual with a broader spectrum of protection against meningococcal infection. It is further optional for a composition or vaccine of the invention to contain a mixture of A proteins from different strains or a mixture of B proteins from different strains, broadening further the potential spectrum of protection conferred by the invention. A still further option is for Tbps to be obtained from or derived from other bacteria, including  N.gonorrhoeae, N.lactamica  and  Moraxella catarrhalis.    
           [0013]    In the present invention, the term “transferrin binding protein” or “Tbp” refers to a protein which either alone binds to transferrin or can be part of a complex of proteins that binds transferrin. The term also embraces fragments, variants and derivatives of such a protein provided that antibodies raised against the fragment, variant or derivative bind the protein. Thus, TbpA and TbpB either dissociated or associated into a complex are considered to be Tbp. Moreover, mutants, fusion proteins or fragments of either TbpA or B or other derivatives of the TbpA+B complex with a common antigenic identity are also considered to be represented by the term Tbp in the present invention.  
           [0014]    A second aspect of the invention provides a composition comprising a complex of two TbpAs and one TbpB. The proteins are thus held together in the ratio seen in the native receptor. The individual proteins may be linked, for example, by hydrogen bonds or covalent bonds. In the latter case, each TbpA is covalently linked to the TbpA, either directly or indirectly. In a preferred embodiment, the complex of TbpA and TbpB assumes a native configuration.  
           [0015]    A native TbpA+B complex may be isolated and purified from  N. meningitidis.  Alternatively, the invention also provides for synthesis of recombinant Tbp protein followed by assembly of the TbpA+B complex in vitro. The TbpA+B complex may be formed by admixture, or may be crosslinked by physical (e.g. UV radiation) or chemical methods known to the art, resulting in a combination of Tbps that will remain together and can not dissociate from each other. In a further example, a single chain recombinant protein comprising two TbpA sequences, preferably in the form of the TbpA dimer, is then covalently linked with TbpB protein to form a complete TbpA+B complex in vitro. Another example of the invention in use provides that TbpA and B are mutated so as to introduce cysteine residues that facilitate the formation of disulphide bridges between the TbpA and TbpB subunits, so as to obtain a covalently bound complex.  
           [0016]    In preparation of a recombinant protein TbpA and TbpB genes may also be truncated so that only those domains known to contribute to the antigenicity of the protein are incorporated in the Tbp complex.  
           [0017]    In some compositions of the invention the TbpA+B complex is able to act as a transferrin receptor and binds to human transferrin. In other compositions of the invention the TbpA+B complex is non-functional in the sense that it does not bind transferrin, but it nevertheless provides an antigenic component that elicits an appropriate immune response.  
           [0018]    A third aspect of the invention provides for a composition comprising a Tbp and  N. meningitidis  outer membrane vesicles. An advantage of this composition is that when administered to a vaccinee or patient it presents a different combination of  N. meningitidis  antigens, and particularly antigens that are in a formation substantially as present on the membrane of live infecting organisms. The combination offers the potential for a more effective protection against infection or a broader spectrum of protection than existing vaccines. Known methods of outer membrane vesicle isolation, such as by desoxycholate treatment, are suitable for preparation of compositions of the invention. In various preferred embodiments the Tbp is Tbp A, Tbp B or TbpA+B either in a native complex or in dissociated form.  
           [0019]    The outer membrane vesicles may further be pretreated in vitro with Tbp so as to enrich the vesicle membrane with Tbp. By “enrich” and like terms, we refer to an outer membrane vesicle to which has been added Tbps so as to increase the concentration or density of Tbps in that vesicle. Preferably, the enrichment results in an outer membrane vesicle having an increased number of transferrin receptors located in the membrane, due to an increased concentration of TbpA and TbpB following their addition to the vesicle and their association into receptors or receptor-like structures. A particular advantage of such embodiments of the invention is that the Tbps, regarded as key antigenic components of the vaccine, are presented in a highly antigenic environment that closely mimics the environment in which transferrin receptors are presented on live, infecting bacteria.  
           [0020]    As mentioned previously, the Tbp components of the composition need not be wild-type Tbps. They can be made recombinantly, and in so doing sequence alterations may be introduced. In one typical example of the invention, recombinant TbpB is modified so as to comprise a membrane binding domain. A preferred membrane spanning domain is a hydrophobic alpha helical region added to either the N or C terminus of the Tbp B protein. However, a membrane anchoring region need not only be an alpha helix, addition of a fatty acid or lipid chain would also facilitate membrane anchoring. In fact, wild type TbpB is believed to anchored to the bacterial outer membrane via such a lipid chain anchor. In a further example of the invention in use, the outer membrane vesicles are pretreated in vitro with membrane binding recombinant TbpB so as to enrich the vesicle membrane with TbpB. OMVs enriched with Tbps are additionally generated by inducing high levels of Tbp expression in  N. meningitidis  and then isolating OMVs via one of the methods described previously. This latter method is typically achieved by transforming the  N. meningitidis  host with a suitable expression vector into which has been inserted a gene or genes encoding the Tbps of choice. Suitable expression vectors for use in neisserial species include the plasmid pMGC10 (Nassif et al. (1991)).  
           [0021]    It is preferred that the composition of the present invention comprises outer membrane vesicles and TbpA+B complexes isolated from a range of different strains of  N. meningitidis.  Other preferred compositions of the invention comprise other  N. meningitidis  proteins including surface antigens, periplasmic proteins, superoxide dismutase and glycoproteins.  
           [0022]    The composition of the third aspect of the invention may instead of or in addition to outer membrane vesicles comprise one or more liposomes, each liposome including TbpA and/or TbpB preferably including TbpA and TbpB associated into a receptor or into a receptor-like complex. Thus a further means of presenting the transferrin receptor antigens is provided.  
           [0023]    In a further embodiment of the third aspect of the invention, the composition comprises 22 kD antigen (Neisserial surface protein A (NspA)) as well as or instead of outer membrane vesicles. NspA and its preparation are described by Martin et al, 1997.  
           [0024]    A fourth aspect of the invention provides for a vaccine comprising a composition of the invention as described above. A vaccine of the invention may also comprise antibodies to Tbp and thus provide a level of passive immunity to bacterial infection.  
           [0025]    A fifth aspect of the invention provides for a method of manufacturing a composition that comprises combining TbpA, TbpB and  N. meningitidis  outer membrane vesicles with a pharmaceutically acceptable carrier. It is preferred that the molar ratio of TbpA to TbpB is about 2:1. The outer membrane vesicles can be pretreated in vitro with native TbpA+B so as to enrich the vesicle membrane with Tbp complex. However, the outer membrane vesicles may also be pretreated with other protein components so as to enrich them for these antigenic components also. The outer membrane vesicles may also be pretreated with antigenic proteins and proteoglycans from several different strains of  N. meningitidis.    
           [0026]    A further aspect of the invention provides for a composition comprising a Tbp and a Cu,Zn-Superoxide dismutase (Cu,Zn-SOD).  
           [0027]    Cu,Zn-Superoxide Dismutase (Cu,Zn-SOD) is an metalloenzyme found in many prokaryotic and eukaryotic organisms. It catalyses the reduction of the superoxide radical anion, O 2   − , to hydrogen peroxide and molecular oxygen, thus playing an important role in the removal of cytotoxic free radicals from the organism. In bacteria Cu,Zn-SODs have been identified in the periplasm of a number of Gram negative species including  N. meningitidis.  The enzyme can exist as a dimer or a monomer, and accordingly in a preferred embodiment the present invention provides for a composition comprising a Tbp and a Cu,Zn-SOD of the dimeric type.  
           [0028]    As mentioned previously with regard to the use of the term “Tbp”, the Cu,Zn-SOD of the present invention is also considered to encompass fragments, variants and derivatives of such a protein provided that antibodies raised against the fragment, variant or derivative bind the wild type Cu,Zn-SOD.  
           [0029]    In examples of the invention in use, compositions are provided that comprise a Cu,Zn-SOD and either a TbpA, a TbpB or a TbpA+B complex. In the latter example it is preferred that the molar ratio of TbpA to Tbp B is between 1.8 and 2.2, with the most suitable compositions having a ratio of 2:1 (TbpA:TbpB). In further compositions of the invention the Tbps are from a different strain of  N. meningitidis  to that of the Cu,Zn-SOD, thus facilitating the formation of a broader spectrum of immune response to meningococcal infection.  
           [0030]    The invention also provides for compositions wherein the Tbps and Cu,Zn-SOD are from different bacterial species, typically different Gram negative species. Such compositions thereby allow an even broader spectrum of immune response to be elicited when administered as a vaccine. Typical compositions comprise a neisserial TbpA+B complex as well as a monomeric Cu,Zn-SOD—from  E. coli  for example—and optionally a further dimeric Cu,Zn-SOD—for example from  Haemophilus parainfluenzae.    
           [0031]    Other aspects of the invention provide for methods of manufacturing compositions that provide protective immunity to Gram negative bacterial infection, comprising combining a covalently linked complex of TbpA and TbpB with either or both of  N. meningitidis  outer membrane vesicles and a Cu,Zn-SOD, plus a pharmaceutically acceptable carrier.  
           [0032]    A further aspect provides for use of Tbps A and B in the manufacture of a medicament for human vaccination. It is preferred that such a medicament is suitable for vaccination against meningococcal infection, although some compositions of the invention provide broad spectrum protection to infection from a wider range of bacterial pathogens. 
       
    
    
     BRIEF DESCRIPTION OF THE FIGURES  
       [0033]    The embodiments of the invention are discussed in more detail by means of the Example described below. The results referred to in the Example are illustrated by the accompanying drawings, in which:  
         [0034]    [0034]FIG. 1 shows immunisation of mice with TbpA+B and outer membrane vesicles;  
         [0035]    [0035]FIG. 2 shows immunisation of mice with TbpA+B;  
         [0036]    [0036]FIG. 3 shows protection of mice against IP infection after immunisation with TbpA+B, isolated TbpA or isolated TbpB;  
         [0037]    [0037]FIGS. 4A and 4B show protection of mice against meningococcal infection following immunisation with either neisserial TbpA+B, TbpB or TbpA (nTbps) or recombinant Tbps (rTbps);  
         [0038]    [0038]FIGS. 5A and 5B show protection of mice against challenges of 10 6  and 10 7  organisms/mouse of  N. meningitidis  strain K454 respectively, following immunisation with recombinant TbpA+B, TbpB or TbpA;  
         [0039]    [0039]FIG. 6 shows protection against challenge with heterologous serogroup;  
         [0040]    [0040]FIG. 7 shows protection against challenge with B16B6;  
         [0041]    [0041]FIG. 8 shows insertion of a promoter construct into the  N. meningitidis  genome for up regulation of endogenous TbpB in  N. meningitidis  by homologous recombination; and  
         [0042]    [0042]FIG. 9. shows insertion of an expression cassette into the  N. meningitidis  genome by homologous recombination. 
     
    
     DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS  
       [0043]    The invention is further illustrated by the following non-limiting examples.  
       EXAMPLES  
     Example 1  
     Mouse Protective Data  
       [0044]    Mice (CAMR-NIH) were immunised with Tbps and/or outer membrane vesicles and challenged with either the homologous or a heterologous meningococcal strain. Survivors per group of mice immunised with Tbps from strain K454 and the outer membrane vesicle vaccine following challenge by strain K454 are shown in FIG. 1. Compared to Tbps, the outer membrane vesicle vaccine gave reduced protection but this may be because it is produced from a different group B strain (H44/76). Animals immunised with TbpA+B isolated from strain K454 were also protected against challenge with other serogroup B organisms (FIG. 1), with greater protection seen with the homologous strain and strains expressing a TbpB with a similar molecular weight. Little or no protection was observed against challenge with meningococci possessing TbpB with a very different molecular weight (strain B16B6). With the heterologous challenge strains, there is a slightly greater number survivors in the groups vaccinated with the combination of Tbps+outer membrane vesicles. However, the numbers involved are small and no definite conclusions can be reached. It is interesting to observe that mice immunised with TbpA+B from strain K454 were also protected against infection with a serogroup C but not a serogroup A strain (FIG. 2).  
         [0045]    Protection against challenge with strain K454 with mice immunised with co-purified TbpA+B and isolated TbpA and TbpB is shown in FIG. 3. It can be seen that TbpB is the predominant antigen responsible for protection, with little protection afforded by TbpA alone.  
         [0046]    Recombinant TbpA and TbpB  
         [0047]    TbpA and TbpB from  N. meningitidis  strain K454 were cloned and overexpressed in  E. coli.  The proteins were purified using affinity chromatography and used to determine their protective potency in a mouse model of meningococcal disease. Recombinant Tbps showed equivalent protection to that provided by Tbps isolated from iron-stressed  N. meningitidis  (FIGS. 4A and 4B). These recombinant Tbps were also utilised in two further larger IP challenge experiments (FIGS.  5 A-B).  
         [0048]    The strong and consistent protective potency of Tbps against mouse I.P. infection with  N. meningitidis  is probably the most compelling evidence for their vaccine potential.  
         [0049]    Interestingly, the results shown in FIGS.  4 B and  5 A-B, although arising from experiments of similar design, show the surprising variability associated with vaccine compositions that depend solely upon either TbpA or TbpB alone. In FIG. 4B the recombinant TbpA composition displayed low levels of protection compared to TbpB or TbpA+B. However, in FIGS. 5A and 5B the recombinant TbpA vaccine composition that showed high levels of protection comparable to the TbpA+B complex and the TbpB alone composition demonstrated poor protection to infection. This latter result is completely contrary to the teaching of the prior art.  
         [0050]    Human Immune Response to TbpA and TbpB in Convalescent Sera  
         [0051]    We have undertaken a number of studies looking at the antibody response in humans to TbpA and TbpB following meningococcal disease. The general conclusions are that both TbpA and TbpB are expressed during meningococcal disease and that an immune response is raised against them. The response is functional (opsonic) and is more cross-reactive between different meningococcal strains than is the response induced by immunisation of animals with Tbps. The immune response to TbpA appears to be stronger and more cross-reactive than that to TbpB, confirming the importance of the vaccine potential of TbpA.  
         [0052]    TbpA and TbpB Form a Transferrin Receptor  
         [0053]    Our structural studies indicate that the transferrin receptor on the meningococcal surface consists of two TbpA molecules and one TbpB molecule that act together.  
         [0054]    Effect of a Vaccine Containing A+B Tbps  
         [0055]    We carried out further tests of the efficacy of recombinant TbpB versus recombinant TbpA+B containing vaccine formulations against challenge from  N. meningitidis  strains L91 705 and B16B6, the results of which are illustrated in FIGS. 6 and 7 respectively. In both cases some improved protection was conferred by A+B compared to B alone.  
       Example 2  
     Up Regulation of Genes in Meningococcal Species  
       [0056]    These methods can be used to enhance expression of TbpB but can equally be applied to other meningococcal outer membrane proteins with known sequence such as NspA (Martin et al., 1997), OMP85 (Manning et al., 1998) or FrpB (Pettersson et al., 1995). Any of the antigenic component sequences disclosed herein (SEQ ID NOS: 1; 3; 5; 7; 9; 11; 13; 15; 17; 19; 21; 23; 25; 27; 29; 31; 33; 35; 37; 39; 41; 43; 45; 47; 49; 51; 53; 55; 57; 59; 61; 63; 65; 67; 69; and 71) would also be suitable for over expression in this system as would any immunogenic meningococcal sequence.  
         [0057]    In the following methods primers are designed using the primer select program from the ‘DNA star’ software package (www.dnastar.com). Primers to flanking sequences are designed following sequencing from the known coding region.  
         [0058]    Up Regulation of TbpB in  N. meningitidis  by Promoter Delivery  
         [0059]    Using homologous recombination (Frosch et al., 1990, van der Ley et al., 1995) a strong promoter such as the porA promoter is inserted into the  N. meningitidis  genome upstream of the tbpB coding region (Legrain et al., 1993). A selectable marker, encoding resistance to kanamycin, is also included in the delivery cassette upstream from the promoter. The integration locus is selected by sequencing upstream from the tbpB coding region.  
         [0060]    PCR primers are designed to the regions flanking the porA promoter and incorporate restriction endonuclease sites for ligation into this amplified sequence. This is then inserted into the multiple cloning site of a vector containing the kanamycin resistance gene. Also inserted through PCR produced products are integration loci that complement a region directly before the coding region for tbpB in the  N. meningitidis  genome. Through homologous recombination the construct shown in FIG. 8 will be produced.  
         [0061]    From the published sequence of porA (McGuinnes et al. 1990) (SEQ ID NO: 1) a primer is constructed at the N-terminal region in order to sequence up steam of the coding region. This sequence data yields the promoter region of the highly expressed porA gene. Primers are designed to this sequence so as to amplify any conserved hexamers centred around −35 and −10 the consensus sequences of TTGACA and TATAAT with a 16-18 bp gap between them and up to 67 bp upstream of the start of transcription (ATG). The designed primers have restriction endonuclease sites incorporated in order to insert the amplified region into a suitable vector.  
         [0062]    In order to identify a suitable integration locus in front of the tbpB coding region a primer is designed to the start of the tbpB coding region, using the published sequence (Legrain et al. 1993), running up stream into the tbpB promoter region. The promoter region itself as well as some sequence upstream is suitable as the integration locus. This section is amplified in two halves using 4 primers and restriction endonucleases are incorporated in order to ligate the sections into a suitable vector.  
         [0063]    The vector used already contains an antibiotic resistance marker (such as kanamycin) and this is suitably positioned between the integration loci but upstream from the incorporated porA promoter, as illustrated in FIG. 8. This means that the vector used has cloning sites either side of the selectable marker.  
         [0064]    Up Regulation of TbpB in  N. meningitidis  by Gene Delivery  
         [0065]    A construct is produced containing a selectable marker, such as kanamycin resistance, up stream from a strong promoter (porA) which is in turn upstream from the coding region for tbpB. Using homologous recombination (Frosch et al., 1990, van der Ley et al., 1995), the cassette is inserted into the  N. meningitidis  genome. The integration locus is selected by sequencing upstream from a known sequenced area that is also known to actively expressed.  
         [0066]    PCR is used to amplify the tbpB coding region and porA promoter region with suitable restriction endonucleases incorporated so they can be inserted in series, into a vector containing kanamycin resistance. Also inserted into the construct are two flanking integration loci complementing a region of the  N. meningitidis  genome (see FIG. 9).  
         [0067]    From the published sequence of porA (McGuinness et al. 1990) (SEQ ID NO: 1) a primer can be constructed to complement the N-terminal region in order to sequence up steam of the coding region. This sequence data yields the promoter region of the highly expressed porA gene. Primers are designed to this sequence so as to amplify any conserved hexamers centred around −35 and −10 the consensus sequences of TTGACA and TATAAT with a 16-18 bp gap between them and up to 67 bp upstream of the start of transcription (ATG). The reverse primer is designed to be as close as possible to the start of the coding region, but does not actually include any of it. This ensures that when it is positioned in series with any inserted DNA it acts as an efficient promoter. The designed primers have restriction endonuclease sites incorporated in order to insert the amplified region into a suitable vector.  
         [0068]    From the published sequence of tbpB (Legrain et al. 1993) primers are constructed to complement the C-terminal and N-terminal regions in order to sequence the flanking regions of the gene. From this sequence primers are constructed which amplify the entire gene. The forward primer incorporates the first coding triplet (ATG) of the gene and does not include the full native promoter region. The reverse is as close to the end of the coding region as possible while still being a good partner to the forward primer. It is accepted that this means the reverse primer may be some distance downstream of the gene of interest.  
         [0069]    From the Foregoing, it will be appreciated that although specific embodiments of the invention have been described herein for purposes of illustration, various modifications may be made without deviating from the spirit and scope of the invention. Accordingly, the invention is not limited except as by the appended claims.  
     
       
       
         1 
         
           
             75  
           
           
             1  
             1176  
             DNA  
             Neisseria meningitidis  
             
               CDS  
               (1)..(1176)  
               porA cds  
             
           
            1 

atg cga aaa aaa ctt acc gcc ctc gta ttg tcc gca ctg ccg ctt gcg       48 
Met Arg Lys Lys Leu Thr Ala Leu Val Leu Ser Ala Leu Pro Leu Ala 
1               5                   10                  15 

gcc gtt gcc gat gtc agc cta tac ggc gaa atc aaa gcc ggc gtg gaa       96 
Ala Val Ala Asp Val Ser Leu Tyr Gly Glu Ile Lys Ala Gly Val Glu 
            20                  25                  30 

ggc agg aac tac cag ctg caa ttg act gaa gca caa gcc gct aac ggt      144 
Gly Arg Asn Tyr Gln Leu Gln Leu Thr Glu Ala Gln Ala Ala Asn Gly 
        35                  40                  45 

gga gcg agc ggt cag gta aaa gtt act aaa gtt act aag gcc aaa agc      192 
Gly Ala Ser Gly Gln Val Lys Val Thr Lys Val Thr Lys Ala Lys Ser 
    50                  55                  60 

cgc atc agg acg aaa atc agt gat ttc ggc tcg ttt atc ggc ttt aag      240 
Arg Ile Arg Thr Lys Ile Ser Asp Phe Gly Ser Phe Ile Gly Phe Lys 
65                  70                  75                  80 

ggg agt gag gat ttg ggc gac ggg ctg aag gct gtt tgg cag ctt gag      288 
Gly Ser Glu Asp Leu Gly Asp Gly Leu Lys Ala Val Trp Gln Leu Glu 
                85                  90                  95 

caa gac gta tcc gtt gcc ggc ggc ggc gcg acc cag tgg ggc aac agg      336 
Gln Asp Val Ser Val Ala Gly Gly Gly Ala Thr Gln Trp Gly Asn Arg 
            100                 105                 110 

gaa tcc ttt atc ggc ttg gca ggc gaa ttc ggt acg ctg cgc gcc ggt      384 
Glu Ser Phe Ile Gly Leu Ala Gly Glu Phe Gly Thr Leu Arg Ala Gly 
        115                 120                 125 

cgc gtt gcg aat cag ttt gac gat gcc agc caa gcc att gat cct tgg      432 
Arg Val Ala Asn Gln Phe Asp Asp Ala Ser Gln Ala Ile Asp Pro Trp 
    130                 135                 140 

gac agc aat aat gat gtg gct tcg caa ttg ggt att ttc aaa cgc cac      480 
Asp Ser Asn Asn Asp Val Ala Ser Gln Leu Gly Ile Phe Lys Arg His 
145                 150                 155                 160 

gac gac atg ccg gtt tcc gta cgc tac gat tcc ccc gaa ttt tcc ggt      528 
Asp Asp Met Pro Val Ser Val Arg Tyr Asp Ser Pro Glu Phe Ser Gly 
                165                 170                 175 

ttc agc ggc agc gtt caa ttc gtt ccg atc caa aac agc aag tcc gcc      576 
Phe Ser Gly Ser Val Gln Phe Val Pro Ile Gln Asn Ser Lys Ser Ala 
            180                 185                 190 

tat acg ccg gct tat tat act aag aat aca aac aat aat ctt act ctc      624 
Tyr Thr Pro Ala Tyr Tyr Thr Lys Asn Thr Asn Asn Asn Leu Thr Leu 
        195                 200                 205 

gtt ccg gct gtt gtc ggc aag ccc gga tcg gat gtg tat tat gcc ggt      672 
Val Pro Ala Val Val Gly Lys Pro Gly Ser Asp Val Tyr Tyr Ala Gly 
    210                 215                 220 

ctg aat tac aaa aat ggc ggt ttt gcc ggg aac tat gcc ttt aaa tat      720 
Leu Asn Tyr Lys Asn Gly Gly Phe Ala Gly Asn Tyr Ala Phe Lys Tyr 
225                 230                 235                 240 

gcg aga cac gcc aat gtc gga cgt aat gct ttt gag ttg ttc ttg atc      768 
Ala Arg His Ala Asn Val Gly Arg Asn Ala Phe Glu Leu Phe Leu Ile 
                245                 250                 255 

ggc agc ggg agt gat caa gcc aaa ggt acc gat ccc ttg aaa aac cat      816 
Gly Ser Gly Ser Asp Gln Ala Lys Gly Thr Asp Pro Leu Lys Asn His 
            260                 265                 270 

cag gta cac cgt ctg acg ggc ggc tat gag gaa ggc ggc ttg aat ctc      864 
Gln Val His Arg Leu Thr Gly Gly Tyr Glu Glu Gly Gly Leu Asn Leu 
        275                 280                 285 

gcc ttg gcg gct cag ttg gat ttg tct gaa aat ggc gac aaa acc aaa      912 
Ala Leu Ala Ala Gln Leu Asp Leu Ser Glu Asn Gly Asp Lys Thr Lys 
    290                 295                 300 

aac agt acg acc gaa att gcc gcc act gct tcc tac cgc ttc ggt aat      960 
Asn Ser Thr Thr Glu Ile Ala Ala Thr Ala Ser Tyr Arg Phe Gly Asn 
305                 310                 315                 320 

gca gtt cca cgc atc agc tat gcc cat ggt ttc gac ttt atc gaa cgc     1008 
Ala Val Pro Arg Ile Ser Tyr Ala His Gly Phe Asp Phe Ile Glu Arg 
                325                 330                 335 

ggt aaa aaa ggc gaa aat acc agc tac gat caa atc atc gcc ggc gtt     1056 
Gly Lys Lys Gly Glu Asn Thr Ser Tyr Asp Gln Ile Ile Ala Gly Val 
            340                 345                 350 

gat tat gat ttt tcc aaa cgc act tcc gcc atc gtg tct ggc gct tgg     1104 
Asp Tyr Asp Phe Ser Lys Arg Thr Ser Ala Ile Val Ser Gly Ala Trp 
        355                 360                 365 

ctg aaa cgc aat acc ggc atc ggc aac tac act caa att aat gcc gcc     1152 
Leu Lys Arg Asn Thr Gly Ile Gly Asn Tyr Thr Gln Ile Asn Ala Ala 
    370                 375                 380 

tcc gtc ggt ttg cgc cac aaa ttc                                     1176 
Ser Val Gly Leu Arg His Lys Phe 
385                 390 

 
           
             2  
             392  
             PRT  
             Neisseria meningitidis  
           
            2 

Met Arg Lys Lys Leu Thr Ala Leu Val Leu Ser Ala Leu Pro Leu Ala 
1               5                   10                  15 

Ala Val Ala Asp Val Ser Leu Tyr Gly Glu Ile Lys Ala Gly Val Glu 
            20                  25                  30 

Gly Arg Asn Tyr Gln Leu Gln Leu Thr Glu Ala Gln Ala Ala Asn Gly 
        35                  40                  45 

Gly Ala Ser Gly Gln Val Lys Val Thr Lys Val Thr Lys Ala Lys Ser 
    50                  55                  60 

Arg Ile Arg Thr Lys Ile Ser Asp Phe Gly Ser Phe Ile Gly Phe Lys 
65                  70                  75                  80 

Gly Ser Glu Asp Leu Gly Asp Gly Leu Lys Ala Val Trp Gln Leu Glu 
                85                  90                  95 

Gln Asp Val Ser Val Ala Gly Gly Gly Ala Thr Gln Trp Gly Asn Arg 
            100                 105                 110 

Glu Ser Phe Ile Gly Leu Ala Gly Glu Phe Gly Thr Leu Arg Ala Gly 
        115                 120                 125 

Arg Val Ala Asn Gln Phe Asp Asp Ala Ser Gln Ala Ile Asp Pro Trp 
    130                 135                 140 

Asp Ser Asn Asn Asp Val Ala Ser Gln Leu Gly Ile Phe Lys Arg His 
145                 150                 155                 160 

Asp Asp Met Pro Val Ser Val Arg Tyr Asp Ser Pro Glu Phe Ser Gly 
                165                 170                 175 

Phe Ser Gly Ser Val Gln Phe Val Pro Ile Gln Asn Ser Lys Ser Ala 
            180                 185                 190 

Tyr Thr Pro Ala Tyr Tyr Thr Lys Asn Thr Asn Asn Asn Leu Thr Leu 
        195                 200                 205 

Val Pro Ala Val Val Gly Lys Pro Gly Ser Asp Val Tyr Tyr Ala Gly 
    210                 215                 220 

Leu Asn Tyr Lys Asn Gly Gly Phe Ala Gly Asn Tyr Ala Phe Lys Tyr 
225                 230                 235                 240 

Ala Arg His Ala Asn Val Gly Arg Asn Ala Phe Glu Leu Phe Leu Ile 
                245                 250                 255 

Gly Ser Gly Ser Asp Gln Ala Lys Gly Thr Asp Pro Leu Lys Asn His 
            260                 265                 270 

Gln Val His Arg Leu Thr Gly Gly Tyr Glu Glu Gly Gly Leu Asn Leu 
        275                 280                 285 

Ala Leu Ala Ala Gln Leu Asp Leu Ser Glu Asn Gly Asp Lys Thr Lys 
    290                 295                 300 

Asn Ser Thr Thr Glu Ile Ala Ala Thr Ala Ser Tyr Arg Phe Gly Asn 
305                 310                 315                 320 

Ala Val Pro Arg Ile Ser Tyr Ala His Gly Phe Asp Phe Ile Glu Arg 
                325                 330                 335 

Gly Lys Lys Gly Glu Asn Thr Ser Tyr Asp Gln Ile Ile Ala Gly Val 
            340                 345                 350 

Asp Tyr Asp Phe Ser Lys Arg Thr Ser Ala Ile Val Ser Gly Ala Trp 
        355                 360                 365 

Leu Lys Arg Asn Thr Gly Ile Gly Asn Tyr Thr Gln Ile Asn Ala Ala 
    370                 375                 380 

Ser Val Gly Leu Arg His Lys Phe 
385                 390 

 
           
             3  
             522  
             DNA  
             Neisseria meningitidis  
             
               CDS  
               (1)..(522)  
               NspA cds  
             
           
            3 

atg aaa aaa gca ctt gcc aca ctg att gcc ctc gct ctc ccg gcc gcc       48 
Met Lys Lys Ala Leu Ala Thr Leu Ile Ala Leu Ala Leu Pro Ala Ala 
1               5                   10                  15 

gca ctg gcg gaa ggc gca tcc ggc ttt tac gtc caa gcc gat gcc gca       96 
Ala Leu Ala Glu Gly Ala Ser Gly Phe Tyr Val Gln Ala Asp Ala Ala 
            20                  25                  30 

cac gca aaa gcc tca agc tct tta ggt tct gcc aaa ggc ttc agc ccg      144 
His Ala Lys Ala Ser Ser Ser Leu Gly Ser Ala Lys Gly Phe Ser Pro 
        35                  40                  45 

cgc atc tcc gca ggc tac cgc atc aac gac ctc cgc ttc gcc gtc gat      192 
Arg Ile Ser Ala Gly Tyr Arg Ile Asn Asp Leu Arg Phe Ala Val Asp 
    50                  55                  60 

tac acg cgc tac aaa aac tat aaa gcc cca tcc acc gat ttc aaa ctt      240 
Tyr Thr Arg Tyr Lys Asn Tyr Lys Ala Pro Ser Thr Asp Phe Lys Leu 
65                  70                  75                  80 

tac agc atc ggc gcg tcc gcc att tac gac ttc gac acc caa tcg ccc      288 
Tyr Ser Ile Gly Ala Ser Ala Ile Tyr Asp Phe Asp Thr Gln Ser Pro 
                85                  90                  95 

gtc aaa ccg tat ctc ggc gcg cgc ttg agc ctc aac cgc gcc tcc gtc      336 
Val Lys Pro Tyr Leu Gly Ala Arg Leu Ser Leu Asn Arg Ala Ser Val 
            100                 105                 110 

gac ttg ggc ggc agc gac agc ttc agc caa acc tcc atc ggc ctc ggc      384 
Asp Leu Gly Gly Ser Asp Ser Phe Ser Gln Thr Ser Ile Gly Leu Gly 
        115                 120                 125 

gta ttg acg ggc gta agc tat gcc gtt acc ccg aat gtc gat ttg gat      432 
Val Leu Thr Gly Val Ser Tyr Ala Val Thr Pro Asn Val Asp Leu Asp 
    130                 135                 140 

gcc ggc tac cgc tac aac tac atc ggc aaa gtc aac act gtc aaa aac      480 
Ala Gly Tyr Arg Tyr Asn Tyr Ile Gly Lys Val Asn Thr Val Lys Asn 
145                 150                 155                 160 

gtc cgt tcc ggc gaa ctg tcc gcc ggt gtg cgc gtc aaa ttc              522 
Val Arg Ser Gly Glu Leu Ser Ala Gly Val Arg Val Lys Phe 
                165                 170 

 
           
             4  
             174  
             PRT  
             Neisseria meningitidis  
           
            4 

Met Lys Lys Ala Leu Ala Thr Leu Ile Ala Leu Ala Leu Pro Ala Ala 
1               5                   10                  15 

Ala Leu Ala Glu Gly Ala Ser Gly Phe Tyr Val Gln Ala Asp Ala Ala 
            20                  25                  30 

His Ala Lys Ala Ser Ser Ser Leu Gly Ser Ala Lys Gly Phe Ser Pro 
        35                  40                  45 

Arg Ile Ser Ala Gly Tyr Arg Ile Asn Asp Leu Arg Phe Ala Val Asp 
    50                  55                  60 

Tyr Thr Arg Tyr Lys Asn Tyr Lys Ala Pro Ser Thr Asp Phe Lys Leu 
65                  70                  75                  80 

Tyr Ser Ile Gly Ala Ser Ala Ile Tyr Asp Phe Asp Thr Gln Ser Pro 
                85                  90                  95 

Val Lys Pro Tyr Leu Gly Ala Arg Leu Ser Leu Asn Arg Ala Ser Val 
            100                 105                 110 

Asp Leu Gly Gly Ser Asp Ser Phe Ser Gln Thr Ser Ile Gly Leu Gly 
        115                 120                 125 

Val Leu Thr Gly Val Ser Tyr Ala Val Thr Pro Asn Val Asp Leu Asp 
    130                 135                 140 

Ala Gly Tyr Arg Tyr Asn Tyr Ile Gly Lys Val Asn Thr Val Lys Asn 
145                 150                 155                 160 

Val Arg Ser Gly Glu Leu Ser Ala Gly Val Arg Val Lys Phe 
                165                 170 

 
           
             5  
             2391  
             DNA  
             Neisseria meningitidis  
             
               CDS  
               (1)..(2391)  
               OMP85 cds  
             
           
            5 

atg aaa ctg aaa cag att gct tcc gca ctg atg atg ttg ggc ata tcg       48 
Met Lys Leu Lys Gln Ile Ala Ser Ala Leu Met Met Leu Gly Ile Ser 
1               5                   10                  15 

cct ttg gca ctt gcc gac ttc acc atc caa gac atc cgc gtc gaa ggc       96 
Pro Leu Ala Leu Ala Asp Phe Thr Ile Gln Asp Ile Arg Val Glu Gly 
            20                  25                  30 

ttg cag cgt acc gag ccg agt acc gta ttc aac tac ctg ccc gtc aaa      144 
Leu Gln Arg Thr Glu Pro Ser Thr Val Phe Asn Tyr Leu Pro Val Lys 
        35                  40                  45 

gtc ggc gac acc tac aac gac aca cac ggc agt gcc atc atc aaa agc      192 
Val Gly Asp Thr Tyr Asn Asp Thr His Gly Ser Ala Ile Ile Lys Ser 
    50                  55                  60 

ctg tac gcc acc ggt ttc ttt gac gac gta cgc gtc gaa act gcg gac      240 
Leu Tyr Ala Thr Gly Phe Phe Asp Asp Val Arg Val Glu Thr Ala Asp 
65                  70                  75                  80 

ggg cag ctc ctg ctg acc gtt atc gaa cgc ccc acc atc ggc tcg ctc      288 
Gly Gln Leu Leu Leu Thr Val Ile Glu Arg Pro Thr Ile Gly Ser Leu 
                85                  90                  95 

aac atc acc ggc gca aaa atg ctg caa aac gac gcc att aag aaa aac      336 
Asn Ile Thr Gly Ala Lys Met Leu Gln Asn Asp Ala Ile Lys Lys Asn 
            100                 105                 110 

ctc gaa tcg ttc ggg ctg gcg cag tcg caa tac ttt aat cag gcg aca      384 
Leu Glu Ser Phe Gly Leu Ala Gln Ser Gln Tyr Phe Asn Gln Ala Thr 
        115                 120                 125 

ctc aat cag gca gtc gcc ggc ctg aaa gaa gaa tac ctc ggg cgc ggc      432 
Leu Asn Gln Ala Val Ala Gly Leu Lys Glu Glu Tyr Leu Gly Arg Gly 
    130                 135                 140 

aaa ctc aat atc caa atc acg ccc aaa gta acc aaa ctc gcc cgc aac      480 
Lys Leu Asn Ile Gln Ile Thr Pro Lys Val Thr Lys Leu Ala Arg Asn 
145                 150                 155                 160 

cgc gtc gac atc gac atc acg att gac gag ggc aaa tcc gcc aaa atc      528 
Arg Val Asp Ile Asp Ile Thr Ile Asp Glu Gly Lys Ser Ala Lys Ile 
                165                 170                 175 

acc gac atc gaa ttt gaa ggc aac caa gtc tat tcc gac cgc aaa ctg      576 
Thr Asp Ile Glu Phe Glu Gly Asn Gln Val Tyr Ser Asp Arg Lys Leu 
            180                 185                 190 

atg cgg caa atg tcc ctg acc gaa ggc ggc att tgg aca tgg ctg aca      624 
Met Arg Gln Met Ser Leu Thr Glu Gly Gly Ile Trp Thr Trp Leu Thr 
        195                 200                 205 

cga agc aac caa ttc aac gag cag aaa ttt gcc caa gat atg gaa aaa      672 
Arg Ser Asn Gln Phe Asn Glu Gln Lys Phe Ala Gln Asp Met Glu Lys 
    210                 215                 220 

gta acc gac ttc tac caa aat aac ggc tac ttc gat ttc cgt atc ctc      720 
Val Thr Asp Phe Tyr Gln Asn Asn Gly Tyr Phe Asp Phe Arg Ile Leu 
225                 230                 235                 240 

gat acc gac atc caa acc aac gaa gac aaa acc aag cag acc atc aaa      768 
Asp Thr Asp Ile Gln Thr Asn Glu Asp Lys Thr Lys Gln Thr Ile Lys 
                245                 250                 255 

atc acc gtc cac gaa ggc gga cgt ttc cgt tgg ggc aaa gtc tcc atc      816 
Ile Thr Val His Glu Gly Gly Arg Phe Arg Trp Gly Lys Val Ser Ile 
            260                 265                 270 

gaa ggc gac acc aac gaa gtc ccc aaa gcc gaa ctg gaa aaa ctg ctg      864 
Glu Gly Asp Thr Asn Glu Val Pro Lys Ala Glu Leu Glu Lys Leu Leu 
        275                 280                 285 

acc atg aag ccc ggc aaa tgg tac gaa cgc cag cag atg acc gcc gtt      912 
Thr Met Lys Pro Gly Lys Trp Tyr Glu Arg Gln Gln Met Thr Ala Val 
    290                 295                 300 

ttg ggt gag att cag aac cgc atg ggc tcg gca ggc tac gca tac agc      960 
Leu Gly Glu Ile Gln Asn Arg Met Gly Ser Ala Gly Tyr Ala Tyr Ser 
305                 310                 315                 320 

gaa atc agc gta cag ccg ctg ccg aac gct gaa acc aaa acc gtc gat     1008 
Glu Ile Ser Val Gln Pro Leu Pro Asn Ala Glu Thr Lys Thr Val Asp 
                325                 330                 335 

ttc gtc ctg cac atc gaa ccg ggc cgg aaa atc tac gtc aac gaa ata     1056 
Phe Val Leu His Ile Glu Pro Gly Arg Lys Ile Tyr Val Asn Glu Ile 
            340                 345                 350 

cac atc acc ggc aac aac aaa acc cgc gac gaa gtc gtc cgc cgt gaa     1104 
His Ile Thr Gly Asn Asn Lys Thr Arg Asp Glu Val Val Arg Arg Glu 
        355                 360                 365 

tta cgc caa atg gaa tcc gca cct tac gac acc tcc aag ctg caa cgt     1152 
Leu Arg Gln Met Glu Ser Ala Pro Tyr Asp Thr Ser Lys Leu Gln Arg 
    370                 375                 380 

tcc aaa gag cgc gtc gag ctt ttg ggc tac ttc gac aat gtc cag ttt     1200 
Ser Lys Glu Arg Val Glu Leu Leu Gly Tyr Phe Asp Asn Val Gln Phe 
385                 390                 395                 400 

gat gct gtc ccg ctt gcc ggc acg ccc gac aaa gtc gat ttg aac atg     1248 
Asp Ala Val Pro Leu Ala Gly Thr Pro Asp Lys Val Asp Leu Asn Met 
                405                 410                 415 

agt ctg acc gaa cgt tcc acc ggt tcc ctg gat ttg agc gcg ggt tgg     1296 
Ser Leu Thr Glu Arg Ser Thr Gly Ser Leu Asp Leu Ser Ala Gly Trp 
            420                 425                 430 

gtt caa gat acc ggg ttg gtc atg tcc gca ggc gtt tcc caa gac aac     1344 
Val Gln Asp Thr Gly Leu Val Met Ser Ala Gly Val Ser Gln Asp Asn 
        435                 440                 445 

ctg ttc ggt acg ggc aag tcg gcc gca ctg cgc gcc tcc agg agc aaa     1392 
Leu Phe Gly Thr Gly Lys Ser Ala Ala Leu Arg Ala Ser Arg Ser Lys 
    450                 455                 460 

acc acg ctt aac ggc tcg ctg tcg ttt act gac ccg tac ttc acg gca     1440 
Thr Thr Leu Asn Gly Ser Leu Ser Phe Thr Asp Pro Tyr Phe Thr Ala 
465                 470                 475                 480 

gac ggg gtc agc ctg ggc tac gat gtt tac gga aaa gcc ttc gac ccg     1488 
Asp Gly Val Ser Leu Gly Tyr Asp Val Tyr Gly Lys Ala Phe Asp Pro 
                485                 490                 495 

cgc aaa gca tcg acc agc atc aaa caa tat aaa acc acc acg gca ggc     1536 
Arg Lys Ala Ser Thr Ser Ile Lys Gln Tyr Lys Thr Thr Thr Ala Gly 
            500                 505                 510 

gca ggc atc cgc atg agc gtg cct gtt acc gaa tac gac cgc gtg aat     1584 
Ala Gly Ile Arg Met Ser Val Pro Val Thr Glu Tyr Asp Arg Val Asn 
        515                 520                 525 

ttc ggt ttg gtg gca gaa cac ctg acc gtc aac acc tac aac aaa gcg     1632 
Phe Gly Leu Val Ala Glu His Leu Thr Val Asn Thr Tyr Asn Lys Ala 
    530                 535                 540 

ccc aaa cac tat gcc gac ttt atc aag aaa tac ggc aaa acc gac ggc     1680 
Pro Lys His Tyr Ala Asp Phe Ile Lys Lys Tyr Gly Lys Thr Asp Gly 
545                 550                 555                 560 

aca gac ggc agc ttc aaa ggc tgg ctg tac aaa ggt acc gtc ggc tgg     1728 
Thr Asp Gly Ser Phe Lys Gly Trp Leu Tyr Lys Gly Thr Val Gly Trp 
                565                 570                 575 

ggg cgc aac aaa acc gac agc gcg tta tgg ccg acg cgc ggc tac ctg     1776 
Gly Arg Asn Lys Thr Asp Ser Ala Leu Trp Pro Thr Arg Gly Tyr Leu 
            580                 585                 590 

acg ggc gtg aac gcc gaa atc gcc ctg cct ggc agc aaa ctg caa tac     1824 
Thr Gly Val Asn Ala Glu Ile Ala Leu Pro Gly Ser Lys Leu Gln Tyr 
        595                 600                 605 

tac tcc gcc acc cac aac caa acc tgg ttc ttc ccc ctg agc aaa acc     1872 
Tyr Ser Ala Thr His Asn Gln Thr Trp Phe Phe Pro Leu Ser Lys Thr 
    610                 615                 620 

ttc acg ctg atg ctc ggc ggc gaa gtc ggc att gcg ggc ggc tac ggc     1920 
Phe Thr Leu Met Leu Gly Gly Glu Val Gly Ile Ala Gly Gly Tyr Gly 
625                 630                 635                 640 

aga acc aaa gaa atc ccc ttc ttt gaa aac ttc tac ggc ggc ggc ctg     1968 
Arg Thr Lys Glu Ile Pro Phe Phe Glu Asn Phe Tyr Gly Gly Gly Leu 
                645                 650                 655 

ggt tcg gtg cgc gga tac gaa agc ggc acg ctc ggt ccg aaa gtc tat     2016 
Gly Ser Val Arg Gly Tyr Glu Ser Gly Thr Leu Gly Pro Lys Val Tyr 
            660                 665                 670 

gac gaa tac ggc gaa aaa atc agc tac ggc ggc aac aaa aaa gcc aac     2064 
Asp Glu Tyr Gly Glu Lys Ile Ser Tyr Gly Gly Asn Lys Lys Ala Asn 
        675                 680                 685 

gtc tcc gcc gag ctg ctc ttc ccg atg ccc ggc gcg aaa gac gcg cgc     2112 
Val Ser Ala Glu Leu Leu Phe Pro Met Pro Gly Ala Lys Asp Ala Arg 
    690                 695                 700 

acc gtc cgc ctg agc ctg ttt gcc gac gca ggc agc gtg tgg gac ggc     2160 
Thr Val Arg Leu Ser Leu Phe Ala Asp Ala Gly Ser Val Trp Asp Gly 
705                 710                 715                 720 

aaa acc tac gac gac aac agc agt tcc gcg acc ggc ggc agg gtt caa     2208 
Lys Thr Tyr Asp Asp Asn Ser Ser Ser Ala Thr Gly Gly Arg Val Gln 
                725                 730                 735 

aac att tac ggc gcc ggc aat acc cat aaa tcc acc ttt acc aac gaa     2256 
Asn Ile Tyr Gly Ala Gly Asn Thr His Lys Ser Thr Phe Thr Asn Glu 
            740                 745                 750 

ttg cgc tat tcc gcc ggc ggc gcg gtt acc tgg ctc tcg cct tta ggc     2304 
Leu Arg Tyr Ser Ala Gly Gly Ala Val Thr Trp Leu Ser Pro Leu Gly 
        755                 760                 765 

ccg atg aaa ttc agc tac gcc tac ccg ctg aag aaa aaa ccg gaa gac     2352 
Pro Met Lys Phe Ser Tyr Ala Tyr Pro Leu Lys Lys Lys Pro Glu Asp 
    770                 775                 780 

gaa atc caa cgc ttc caa ttc caa ctc ggc acg acg ttc                 2391 
Glu Ile Gln Arg Phe Gln Phe Gln Leu Gly Thr Thr Phe 
785                 790                 795 

 
           
             6  
             797  
             PRT  
             Neisseria meningitidis  
           
            6 

Met Lys Leu Lys Gln Ile Ala Ser Ala Leu Met Met Leu Gly Ile Ser 
1               5                   10                  15 

Pro Leu Ala Leu Ala Asp Phe Thr Ile Gln Asp Ile Arg Val Glu Gly 
            20                  25                  30 

Leu Gln Arg Thr Glu Pro Ser Thr Val Phe Asn Tyr Leu Pro Val Lys 
        35                  40                  45 

Val Gly Asp Thr Tyr Asn Asp Thr His Gly Ser Ala Ile Ile Lys Ser 
    50                  55                  60 

Leu Tyr Ala Thr Gly Phe Phe Asp Asp Val Arg Val Glu Thr Ala Asp 
65                  70                  75                  80 

Gly Gln Leu Leu Leu Thr Val Ile Glu Arg Pro Thr Ile Gly Ser Leu 
                85                  90                  95 

Asn Ile Thr Gly Ala Lys Met Leu Gln Asn Asp Ala Ile Lys Lys Asn 
            100                 105                 110 

Leu Glu Ser Phe Gly Leu Ala Gln Ser Gln Tyr Phe Asn Gln Ala Thr 
        115                 120                 125 

Leu Asn Gln Ala Val Ala Gly Leu Lys Glu Glu Tyr Leu Gly Arg Gly 
    130                 135                 140 

Lys Leu Asn Ile Gln Ile Thr Pro Lys Val Thr Lys Leu Ala Arg Asn 
145                 150                 155                 160 

Arg Val Asp Ile Asp Ile Thr Ile Asp Glu Gly Lys Ser Ala Lys Ile 
                165                 170                 175 

Thr Asp Ile Glu Phe Glu Gly Asn Gln Val Tyr Ser Asp Arg Lys Leu 
            180                 185                 190 

Met Arg Gln Met Ser Leu Thr Glu Gly Gly Ile Trp Thr Trp Leu Thr 
        195                 200                 205 

Arg Ser Asn Gln Phe Asn Glu Gln Lys Phe Ala Gln Asp Met Glu Lys 
    210                 215                 220 

Val Thr Asp Phe Tyr Gln Asn Asn Gly Tyr Phe Asp Phe Arg Ile Leu 
225                 230                 235                 240 

Asp Thr Asp Ile Gln Thr Asn Glu Asp Lys Thr Lys Gln Thr Ile Lys 
                245                 250                 255 

Ile Thr Val His Glu Gly Gly Arg Phe Arg Trp Gly Lys Val Ser Ile 
            260                 265                 270 

Glu Gly Asp Thr Asn Glu Val Pro Lys Ala Glu Leu Glu Lys Leu Leu 
        275                 280                 285 

Thr Met Lys Pro Gly Lys Trp Tyr Glu Arg Gln Gln Met Thr Ala Val 
    290                 295                 300 

Leu Gly Glu Ile Gln Asn Arg Met Gly Ser Ala Gly Tyr Ala Tyr Ser 
305                 310                 315                 320 

Glu Ile Ser Val Gln Pro Leu Pro Asn Ala Glu Thr Lys Thr Val Asp 
                325                 330                 335 

Phe Val Leu His Ile Glu Pro Gly Arg Lys Ile Tyr Val Asn Glu Ile 
            340                 345                 350 

His Ile Thr Gly Asn Asn Lys Thr Arg Asp Glu Val Val Arg Arg Glu 
        355                 360                 365 

Leu Arg Gln Met Glu Ser Ala Pro Tyr Asp Thr Ser Lys Leu Gln Arg 
    370                 375                 380 

Ser Lys Glu Arg Val Glu Leu Leu Gly Tyr Phe Asp Asn Val Gln Phe 
385                 390                 395                 400 

Asp Ala Val Pro Leu Ala Gly Thr Pro Asp Lys Val Asp Leu Asn Met 
                405                 410                 415 

Ser Leu Thr Glu Arg Ser Thr Gly Ser Leu Asp Leu Ser Ala Gly Trp 
            420                 425                 430 

Val Gln Asp Thr Gly Leu Val Met Ser Ala Gly Val Ser Gln Asp Asn 
        435                 440                 445 

Leu Phe Gly Thr Gly Lys Ser Ala Ala Leu Arg Ala Ser Arg Ser Lys 
    450                 455                 460 

Thr Thr Leu Asn Gly Ser Leu Ser Phe Thr Asp Pro Tyr Phe Thr Ala 
465                 470                 475                 480 

Asp Gly Val Ser Leu Gly Tyr Asp Val Tyr Gly Lys Ala Phe Asp Pro 
                485                 490                 495 

Arg Lys Ala Ser Thr Ser Ile Lys Gln Tyr Lys Thr Thr Thr Ala Gly 
            500                 505                 510 

Ala Gly Ile Arg Met Ser Val Pro Val Thr Glu Tyr Asp Arg Val Asn 
        515                 520                 525 

Phe Gly Leu Val Ala Glu His Leu Thr Val Asn Thr Tyr Asn Lys Ala 
    530                 535                 540 

Pro Lys His Tyr Ala Asp Phe Ile Lys Lys Tyr Gly Lys Thr Asp Gly 
545                 550                 555                 560 

Thr Asp Gly Ser Phe Lys Gly Trp Leu Tyr Lys Gly Thr Val Gly Trp 
                565                 570                 575 

Gly Arg Asn Lys Thr Asp Ser Ala Leu Trp Pro Thr Arg Gly Tyr Leu 
            580                 585                 590 

Thr Gly Val Asn Ala Glu Ile Ala Leu Pro Gly Ser Lys Leu Gln Tyr 
        595                 600                 605 

Tyr Ser Ala Thr His Asn Gln Thr Trp Phe Phe Pro Leu Ser Lys Thr 
    610                 615                 620 

Phe Thr Leu Met Leu Gly Gly Glu Val Gly Ile Ala Gly Gly Tyr Gly 
625                 630                 635                 640 

Arg Thr Lys Glu Ile Pro Phe Phe Glu Asn Phe Tyr Gly Gly Gly Leu 
                645                 650                 655 

Gly Ser Val Arg Gly Tyr Glu Ser Gly Thr Leu Gly Pro Lys Val Tyr 
            660                 665                 670 

Asp Glu Tyr Gly Glu Lys Ile Ser Tyr Gly Gly Asn Lys Lys Ala Asn 
        675                 680                 685 

Val Ser Ala Glu Leu Leu Phe Pro Met Pro Gly Ala Lys Asp Ala Arg 
    690                 695                 700 

Thr Val Arg Leu Ser Leu Phe Ala Asp Ala Gly Ser Val Trp Asp Gly 
705                 710                 715                 720 

Lys Thr Tyr Asp Asp Asn Ser Ser Ser Ala Thr Gly Gly Arg Val Gln 
                725                 730                 735 

Asn Ile Tyr Gly Ala Gly Asn Thr His Lys Ser Thr Phe Thr Asn Glu 
            740                 745                 750 

Leu Arg Tyr Ser Ala Gly Gly Ala Val Thr Trp Leu Ser Pro Leu Gly 
        755                 760                 765 

Pro Met Lys Phe Ser Tyr Ala Tyr Pro Leu Lys Lys Lys Pro Glu Asp 
    770                 775                 780 

Glu Ile Gln Arg Phe Gln Phe Gln Leu Gly Thr Thr Phe 
785                 790                 795 

 
           
             7  
             2142  
             DNA  
             Neisseria meningitidis  
             
               CDS  
               (1)..(2142)  
               FrpB cds  
             
           
            7 

atg aat acc cca ttg ttc cgt ctc agc ctg ctc tcg ctt acc ctg gcg       48 
Met Asn Thr Pro Leu Phe Arg Leu Ser Leu Leu Ser Leu Thr Leu Ala 
1               5                   10                  15 

gca ggt ttt gcc cat gcg gca gaa aat aat gcc aag gtc gta ctg gat       96 
Ala Gly Phe Ala His Ala Ala Glu Asn Asn Ala Lys Val Val Leu Asp 
            20                  25                  30 

acc gtt acc gta aaa ggc gac cgc caa ggc agc aaa atc cgt acc aac      144 
Thr Val Thr Val Lys Gly Asp Arg Gln Gly Ser Lys Ile Arg Thr Asn 
        35                  40                  45 

atc gtt acg ctg caa caa aaa gac gaa agc acc gca acc gat atg cgc      192 
Ile Val Thr Leu Gln Gln Lys Asp Glu Ser Thr Ala Thr Asp Met Arg 
    50                  55                  60 

gaa ctc tta aaa gaa gag ccc tcc atc gat ttc ggc ggc ggc aac ggc      240 
Glu Leu Leu Lys Glu Glu Pro Ser Ile Asp Phe Gly Gly Gly Asn Gly 
65                  70                  75                  80 

acg tcc caa ttc ctg acg ctg cgc ggc atg ggt caa aac tct gtc gac      288 
Thr Ser Gln Phe Leu Thr Leu Arg Gly Met Gly Gln Asn Ser Val Asp 
                85                  90                  95 

atc aag gtg gac aac gcc tat tcc gac agc caa atc ctt tac cac caa      336 
Ile Lys Val Asp Asn Ala Tyr Ser Asp Ser Gln Ile Leu Tyr His Gln 
            100                 105                 110 

ggc aga ttt att gtc gat ccc gct ttg gtt aaa gtc gtt tcc gta caa      384 
Gly Arg Phe Ile Val Asp Pro Ala Leu Val Lys Val Val Ser Val Gln 
        115                 120                 125 

aaa ggc gcg ggt tcc gcc tct gcc ggt atc ggc gcg acc aac ggc gcg      432 
Lys Gly Ala Gly Ser Ala Ser Ala Gly Ile Gly Ala Thr Asn Gly Ala 
    130                 135                 140 

atc atc acc aaa acc gtc gat gcc caa gac ctg ctc aaa ggc ttg gat      480 
Ile Ile Thr Lys Thr Val Asp Ala Gln Asp Leu Leu Lys Gly Leu Asp 
145                 150                 155                 160 

aaa aac tgg ggc gtg cgc ctc aac agc ggc ttt gcc agc aac gaa ggc      528 
Lys Asn Trp Gly Val Arg Leu Asn Ser Gly Phe Ala Ser Asn Glu Gly 
                165                 170                 175 

gta agc tac ggc gca agc gta ttc ggg aaa gag ggc aac ttc gac ggc      576 
Val Ser Tyr Gly Ala Ser Val Phe Gly Lys Glu Gly Asn Phe Asp Gly 
            180                 185                 190 

ttg ttc tct tac aac cgc aac aat gaa aaa gat tac gaa gca ggt aaa      624 
Leu Phe Ser Tyr Asn Arg Asn Asn Glu Lys Asp Tyr Glu Ala Gly Lys 
        195                 200                 205 

ggc ttc cgt aat aat ttc aac ggc ggc aaa acc gta ccg tac agc gcg      672 
Gly Phe Arg Asn Asn Phe Asn Gly Gly Lys Thr Val Pro Tyr Ser Ala 
    210                 215                 220 

ctg gac aaa cgc agc tac ctc gcc aaa atc gga aca agc ttc ggc gac      720 
Leu Asp Lys Arg Ser Tyr Leu Ala Lys Ile Gly Thr Ser Phe Gly Asp 
225                 230                 235                 240 

ggc gac cac cgc atc gta ttg agc cat atg aaa gac cag cac cgg ggc      768 
Gly Asp His Arg Ile Val Leu Ser His Met Lys Asp Gln His Arg Gly 
                245                 250                 255 

atc cgt acc gtc cgt gaa gaa ttt acc gtc ggc ggc gat aaa gag cga      816 
Ile Arg Thr Val Arg Glu Glu Phe Thr Val Gly Gly Asp Lys Glu Arg 
            260                 265                 270 

ata agt atg gaa cgc caa gcc cct gct tac cgc gaa acc aca caa tcc      864 
Ile Ser Met Glu Arg Gln Ala Pro Ala Tyr Arg Glu Thr Thr Gln Ser 
        275                 280                 285 

aac acc aat ttg gcg tac acg ggt aaa aac ctg ggc ttt gtc gaa aaa      912 
Asn Thr Asn Leu Ala Tyr Thr Gly Lys Asn Leu Gly Phe Val Glu Lys 
    290                 295                 300 

ctg gat gcc aac gcc tat gtg ttg gaa aaa gaa cgc tat tcc gcc gat      960 
Leu Asp Ala Asn Ala Tyr Val Leu Glu Lys Glu Arg Tyr Ser Ala Asp 
305                 310                 315                 320 

gac agc ggc acc ggt tac gca ggc aat gta aaa ggc ccc aac cat acc     1008 
Asp Ser Gly Thr Gly Tyr Ala Gly Asn Val Lys Gly Pro Asn His Thr 
                325                 330                 335 

caa atc acc act cgg ggt atg aac ttc aac ttc gac agc cgc ctt gcc     1056 
Gln Ile Thr Thr Arg Gly Met Asn Phe Asn Phe Asp Ser Arg Leu Ala 
            340                 345                 350 

gaa caa acc ctg ctg aaa tac ggt atc aac tac cgc cat cag gaa atc     1104 
Glu Gln Thr Leu Leu Lys Tyr Gly Ile Asn Tyr Arg His Gln Glu Ile 
        355                 360                 365 

aaa ccg caa gcg ttt ttg aat tca caa ttt aaa att gaa gat aaa gaa     1152 
Lys Pro Gln Ala Phe Leu Asn Ser Gln Phe Lys Ile Glu Asp Lys Glu 
    370                 375                 380 

aaa gca act gat gaa gag aaa aat aag aac cgt gaa aat gaa aaa att     1200 
Lys Ala Thr Asp Glu Glu Lys Asn Lys Asn Arg Glu Asn Glu Lys Ile 
385                 390                 395                 400 

gcc aaa gcc tac cgt ctg acc aac ccg acc aaa acc gat acc ggc gcg     1248 
Ala Lys Ala Tyr Arg Leu Thr Asn Pro Thr Lys Thr Asp Thr Gly Ala 
                405                 410                 415 

tat atc gaa gcc att cac gag att gac ggc ttt acc ctg acc ggc ggg     1296 
Tyr Ile Glu Ala Ile His Glu Ile Asp Gly Phe Thr Leu Thr Gly Gly 
            420                 425                 430 

ctg cgt tac gac cgc ttc aag gtg aaa acc cac gac ggc aaa acc gtt     1344 
Leu Arg Tyr Asp Arg Phe Lys Val Lys Thr His Asp Gly Lys Thr Val 
        435                 440                 445 

tca agc aac aac ctt aac ccg agt ttc ggc gtg att tgg cag ccg cac     1392 
Ser Ser Asn Asn Leu Asn Pro Ser Phe Gly Val Ile Trp Gln Pro His 
    450                 455                 460 

gaa cac tgg agc ttc agc gcg agc cac aac tac gcc agc cgc agc ccg     1440 
Glu His Trp Ser Phe Ser Ala Ser His Asn Tyr Ala Ser Arg Ser Pro 
465                 470                 475                 480 

cgc ctg tat gac gcg ctg caa acc cac ggc aaa cgc ggc atc atc tcg     1488 
Arg Leu Tyr Asp Ala Leu Gln Thr His Gly Lys Arg Gly Ile Ile Ser 
                485                 490                 495 

att gcc gac ggc acg aaa gcc gaa cgc gcg cgc aat acc gaa atc ggc     1536 
Ile Ala Asp Gly Thr Lys Ala Glu Arg Ala Arg Asn Thr Glu Ile Gly 
            500                 505                 510 

ttc aac tac aac gac ggc acg ttt gcc gca aac ggc agc tac ttc tgg     1584 
Phe Asn Tyr Asn Asp Gly Thr Phe Ala Ala Asn Gly Ser Tyr Phe Trp 
        515                 520                 525 

cag acc atc aaa gac gcg ctt gcc aat ccg caa aac cgc cac gac tct     1632 
Gln Thr Ile Lys Asp Ala Leu Ala Asn Pro Gln Asn Arg His Asp Ser 
    530                 535                 540 

gtc gcc gtc cgt gaa gcc gtc aat gcc ggt tac atc aaa aac cac ggt     1680 
Val Ala Val Arg Glu Ala Val Asn Ala Gly Tyr Ile Lys Asn His Gly 
545                 550                 555                 560 

tac gaa ttg ggc gcg tcc tac cgc acc ggc ggc ctg act gcc aaa gtc     1728 
Tyr Glu Leu Gly Ala Ser Tyr Arg Thr Gly Gly Leu Thr Ala Lys Val 
                565                 570                 575 

ggc gta agc cac agc aaa ccg cgc ttt tac gat acg cac aaa gac aag     1776 
Gly Val Ser His Ser Lys Pro Arg Phe Tyr Asp Thr His Lys Asp Lys 
            580                 585                 590 

ctg ttg agc gcg aat cct gaa ttt ggc gca caa gtc ggc cgc act tgg     1824 
Leu Leu Ser Ala Asn Pro Glu Phe Gly Ala Gln Val Gly Arg Thr Trp 
        595                 600                 605 

acg gct tcc ctt gcc tac cgc ttc caa aac ccg aat ctg gaa atc ggc     1872 
Thr Ala Ser Leu Ala Tyr Arg Phe Gln Asn Pro Asn Leu Glu Ile Gly 
    610                 615                 620 

tgg cgc ggc cgt tat gtt caa aaa gcc gtg ggt tcg ata ttg gtg gca     1920 
Trp Arg Gly Arg Tyr Val Gln Lys Ala Val Gly Ser Ile Leu Val Ala 
625                 630                 635                 640 

ggt caa aaa gac cgc aac ggc aaa ttg gaa aac gtt gta cgc aaa ggt     1968 
Gly Gln Lys Asp Arg Asn Gly Lys Leu Glu Asn Val Val Arg Lys Gly 
                645                 650                 655 

ttc ggt gtg aac gat gtc ttc gcc aac tgg aaa ccg ctg ggc aaa gac     2016 
Phe Gly Val Asn Asp Val Phe Ala Asn Trp Lys Pro Leu Gly Lys Asp 
            660                 665                 670 

acg ctc aat gtt aat ctt tcg gtt aac aac gtg ttc aac acg ttc tac     2064 
Thr Leu Asn Val Asn Leu Ser Val Asn Asn Val Phe Asn Thr Phe Tyr 
        675                 680                 685 

tat ccg cac agc caa cga tgg acc aat acc ctg ccg ggc gtg gga cgt     2112 
Tyr Pro His Ser Gln Arg Trp Thr Asn Thr Leu Pro Gly Val Gly Arg 
    690                 695                 700 

gat gta cgc ttg ggc gtg aac tac aag ttc                             2142 
Asp Val Arg Leu Gly Val Asn Tyr Lys Phe 
705                 710 

 
           
             8  
             714  
             PRT  
             Neisseria meningitidis  
           
            8 

Met Asn Thr Pro Leu Phe Arg Leu Ser Leu Leu Ser Leu Thr Leu Ala 
1               5                   10                  15 

Ala Gly Phe Ala His Ala Ala Glu Asn Asn Ala Lys Val Val Leu Asp 
            20                  25                  30 

Thr Val Thr Val Lys Gly Asp Arg Gln Gly Ser Lys Ile Arg Thr Asn 
        35                  40                  45 

Ile Val Thr Leu Gln Gln Lys Asp Glu Ser Thr Ala Thr Asp Met Arg 
    50                  55                  60 

Glu Leu Leu Lys Glu Glu Pro Ser Ile Asp Phe Gly Gly Gly Asn Gly 
65                  70                  75                  80 

Thr Ser Gln Phe Leu Thr Leu Arg Gly Met Gly Gln Asn Ser Val Asp 
                85                  90                  95 

Ile Lys Val Asp Asn Ala Tyr Ser Asp Ser Gln Ile Leu Tyr His Gln 
            100                 105                 110 

Gly Arg Phe Ile Val Asp Pro Ala Leu Val Lys Val Val Ser Val Gln 
        115                 120                 125 

Lys Gly Ala Gly Ser Ala Ser Ala Gly Ile Gly Ala Thr Asn Gly Ala 
    130                 135                 140 

Ile Ile Thr Lys Thr Val Asp Ala Gln Asp Leu Leu Lys Gly Leu Asp 
145                 150                 155                 160 

Lys Asn Trp Gly Val Arg Leu Asn Ser Gly Phe Ala Ser Asn Glu Gly 
                165                 170                 175 

Val Ser Tyr Gly Ala Ser Val Phe Gly Lys Glu Gly Asn Phe Asp Gly 
            180                 185                 190 

Leu Phe Ser Tyr Asn Arg Asn Asn Glu Lys Asp Tyr Glu Ala Gly Lys 
        195                 200                 205 

Gly Phe Arg Asn Asn Phe Asn Gly Gly Lys Thr Val Pro Tyr Ser Ala 
    210                 215                 220 

Leu Asp Lys Arg Ser Tyr Leu Ala Lys Ile Gly Thr Ser Phe Gly Asp 
225                 230                 235                 240 

Gly Asp His Arg Ile Val Leu Ser His Met Lys Asp Gln His Arg Gly 
                245                 250                 255 

Ile Arg Thr Val Arg Glu Glu Phe Thr Val Gly Gly Asp Lys Glu Arg 
            260                 265                 270 

Ile Ser Met Glu Arg Gln Ala Pro Ala Tyr Arg Glu Thr Thr Gln Ser 
        275                 280                 285 

Asn Thr Asn Leu Ala Tyr Thr Gly Lys Asn Leu Gly Phe Val Glu Lys 
    290                 295                 300 

Leu Asp Ala Asn Ala Tyr Val Leu Glu Lys Glu Arg Tyr Ser Ala Asp 
305                 310                 315                 320 

Asp Ser Gly Thr Gly Tyr Ala Gly Asn Val Lys Gly Pro Asn His Thr 
                325                 330                 335 

Gln Ile Thr Thr Arg Gly Met Asn Phe Asn Phe Asp Ser Arg Leu Ala 
            340                 345                 350 

Glu Gln Thr Leu Leu Lys Tyr Gly Ile Asn Tyr Arg His Gln Glu Ile 
        355                 360                 365 

Lys Pro Gln Ala Phe Leu Asn Ser Gln Phe Lys Ile Glu Asp Lys Glu 
    370                 375                 380 

Lys Ala Thr Asp Glu Glu Lys Asn Lys Asn Arg Glu Asn Glu Lys Ile 
385                 390                 395                 400 

Ala Lys Ala Tyr Arg Leu Thr Asn Pro Thr Lys Thr Asp Thr Gly Ala 
                405                 410                 415 

Tyr Ile Glu Ala Ile His Glu Ile Asp Gly Phe Thr Leu Thr Gly Gly 
            420                 425                 430 

Leu Arg Tyr Asp Arg Phe Lys Val Lys Thr His Asp Gly Lys Thr Val 
        435                 440                 445 

Ser Ser Asn Asn Leu Asn Pro Ser Phe Gly Val Ile Trp Gln Pro His 
    450                 455                 460 

Glu His Trp Ser Phe Ser Ala Ser His Asn Tyr Ala Ser Arg Ser Pro 
465                 470                 475                 480 

Arg Leu Tyr Asp Ala Leu Gln Thr His Gly Lys Arg Gly Ile Ile Ser 
                485                 490                 495 

Ile Ala Asp Gly Thr Lys Ala Glu Arg Ala Arg Asn Thr Glu Ile Gly 
            500                 505                 510 

Phe Asn Tyr Asn Asp Gly Thr Phe Ala Ala Asn Gly Ser Tyr Phe Trp 
        515                 520                 525 

Gln Thr Ile Lys Asp Ala Leu Ala Asn Pro Gln Asn Arg His Asp Ser 
    530                 535                 540 

Val Ala Val Arg Glu Ala Val Asn Ala Gly Tyr Ile Lys Asn His Gly 
545                 550                 555                 560 

Tyr Glu Leu Gly Ala Ser Tyr Arg Thr Gly Gly Leu Thr Ala Lys Val 
                565                 570                 575 

Gly Val Ser His Ser Lys Pro Arg Phe Tyr Asp Thr His Lys Asp Lys 
            580                 585                 590 

Leu Leu Ser Ala Asn Pro Glu Phe Gly Ala Gln Val Gly Arg Thr Trp 
        595                 600                 605 

Thr Ala Ser Leu Ala Tyr Arg Phe Gln Asn Pro Asn Leu Glu Ile Gly 
    610                 615                 620 

Trp Arg Gly Arg Tyr Val Gln Lys Ala Val Gly Ser Ile Leu Val Ala 
625                 630                 635                 640 

Gly Gln Lys Asp Arg Asn Gly Lys Leu Glu Asn Val Val Arg Lys Gly 
                645                 650                 655 

Phe Gly Val Asn Asp Val Phe Ala Asn Trp Lys Pro Leu Gly Lys Asp 
            660                 665                 670 

Thr Leu Asn Val Asn Leu Ser Val Asn Asn Val Phe Asn Thr Phe Tyr 
        675                 680                 685 

Tyr Pro His Ser Gln Arg Trp Thr Asn Thr Leu Pro Gly Val Gly Arg 
    690                 695                 700 

Asp Val Arg Leu Gly Val Asn Tyr Lys Phe 
705                 710 

 
           
             9  
             2307  
             DNA  
             Neisseria meningitidis  
             
               CDS  
               (1)..(2307)  
               PilQ cds  
             
           
            9 

atg aat acc aaa ctg aca aaa atc att tcc ggt ctc ttt gtc gca acc       48 
Met Asn Thr Lys Leu Thr Lys Ile Ile Ser Gly Leu Phe Val Ala Thr 
1               5                   10                  15 

gcc gcc ttt cag aca gca tcg gca gga aac att aca gac atc aaa gtt       96 
Ala Ala Phe Gln Thr Ala Ser Ala Gly Asn Ile Thr Asp Ile Lys Val 
            20                  25                  30 

tcc tcc ctg ccc aac aaa cag aaa atc gtc aaa gtc agc ttt gac aaa      144 
Ser Ser Leu Pro Asn Lys Gln Lys Ile Val Lys Val Ser Phe Asp Lys 
        35                  40                  45 

gag att gtc aac ccg acc ggc ttc gta acc tcc tca ccg gcc cgc atc      192 
Glu Ile Val Asn Pro Thr Gly Phe Val Thr Ser Ser Pro Ala Arg Ile 
    50                  55                  60 

gcc ttg gac ttt gaa caa acc ggc att tcc atg gat caa cag gta ctc      240 
Ala Leu Asp Phe Glu Gln Thr Gly Ile Ser Met Asp Gln Gln Val Leu 
65                  70                  75                  80 

gaa tat gcc gat cct ctg ttg agc aaa atc agt gcc gca caa aac agc      288 
Glu Tyr Ala Asp Pro Leu Leu Ser Lys Ile Ser Ala Ala Gln Asn Ser 
                85                  90                  95 

agc cgt gcg cgt ctg gtt ctg aat ctg aac aaa ccg ggc caa tac aat      336 
Ser Arg Ala Arg Leu Val Leu Asn Leu Asn Lys Pro Gly Gln Tyr Asn 
            100                 105                 110 

acc gaa gta cgc ggg aac aaa gtt tgg ata ttc att aac gaa tcg gac      384 
Thr Glu Val Arg Gly Asn Lys Val Trp Ile Phe Ile Asn Glu Ser Asp 
        115                 120                 125 

gat acc gtg tcc gcc ccc gca cgc ccc gcc gta aaa gcc gcg cct gcc      432 
Asp Thr Val Ser Ala Pro Ala Arg Pro Ala Val Lys Ala Ala Pro Ala 
    130                 135                 140 

gca ccg gca aaa caa cag gct gcc gca ccg tct acc aag tcc gca gta      480 
Ala Pro Ala Lys Gln Gln Ala Ala Ala Pro Ser Thr Lys Ser Ala Val 
145                 150                 155                 160 

tcc gta tcc gaa ccc ttt acc ccg gca aaa caa cag gct gcc gca ccg      528 
Ser Val Ser Glu Pro Phe Thr Pro Ala Lys Gln Gln Ala Ala Ala Pro 
                165                 170                 175 

ttt acc gag tcc gta gta tcc gta tcc gca ccg ttc agc ccg gca aaa      576 
Phe Thr Glu Ser Val Val Ser Val Ser Ala Pro Phe Ser Pro Ala Lys 
            180                 185                 190 

caa cag gcg gcg gca tca gca aaa caa cag gcg gca gca cca gca aaa      624 
Gln Gln Ala Ala Ala Ser Ala Lys Gln Gln Ala Ala Ala Pro Ala Lys 
        195                 200                 205 

caa cag gcg gca gca cca gca aaa caa cag gcg gca gca cca gca aaa      672 
Gln Gln Ala Ala Ala Pro Ala Lys Gln Gln Ala Ala Ala Pro Ala Lys 
    210                 215                 220 

caa acc aat atc gat ttc cgc aaa gac ggc aaa aat gcc ggc att atc      720 
Gln Thr Asn Ile Asp Phe Arg Lys Asp Gly Lys Asn Ala Gly Ile Ile 
225                 230                 235                 240 

gaa ttg gct gca ttg ggc ttt gcc ggg cag ccc gac atc agc caa cag      768 
Glu Leu Ala Ala Leu Gly Phe Ala Gly Gln Pro Asp Ile Ser Gln Gln 
                245                 250                 255 

cac gac cac atc atc gtt acg ctg aaa aac cat acc ctg ccg acc acg      816 
His Asp His Ile Ile Val Thr Leu Lys Asn His Thr Leu Pro Thr Thr 
            260                 265                 270 

ctc caa cgc agt ttg gat gtg gca gac ttt aaa aca ccg gtt caa aag      864 
Leu Gln Arg Ser Leu Asp Val Ala Asp Phe Lys Thr Pro Val Gln Lys 
        275                 280                 285 

gtt acg ctg aaa cgc ctc aat aac gac acc cag ctg att atc aca aca      912 
Val Thr Leu Lys Arg Leu Asn Asn Asp Thr Gln Leu Ile Ile Thr Thr 
    290                 295                 300 

gcc ggc aac tgg gaa ctc gtc aac aaa tcc gcc gcg ccc gga tac ttt      960 
Ala Gly Asn Trp Glu Leu Val Asn Lys Ser Ala Ala Pro Gly Tyr Phe 
305                 310                 315                 320 

acc ttc caa gtc ctg ccg aaa aaa caa aac ctc gag tca ggc ggc gtg     1008 
Thr Phe Gln Val Leu Pro Lys Lys Gln Asn Leu Glu Ser Gly Gly Val 
                325                 330                 335 

aac aat gcg ccc aaa acc ttc aca ggc cgg aaa atc tcc ctt gac ttc     1056 
Asn Asn Ala Pro Lys Thr Phe Thr Gly Arg Lys Ile Ser Leu Asp Phe 
            340                 345                 350 

caa gat gtc gaa atc cgc acc atc ctg cag att ttg gca aaa gaa tcc     1104 
Gln Asp Val Glu Ile Arg Thr Ile Leu Gln Ile Leu Ala Lys Glu Ser 
        355                 360                 365 

gga atg aac att gtt gcc agc gac tcc gtc aac ggc aaa atg acc ctc     1152 
Gly Met Asn Ile Val Ala Ser Asp Ser Val Asn Gly Lys Met Thr Leu 
    370                 375                 380 

tcc ctc aag gat gtg cct tgg gat cag gct ttg gat ttg gtt atg cag     1200 
Ser Leu Lys Asp Val Pro Trp Asp Gln Ala Leu Asp Leu Val Met Gln 
385                 390                 395                 400 

gcg cgc aac ctc gat atg cgc cag caa ggg aat atc gtc aac atc gcg     1248 
Ala Arg Asn Leu Asp Met Arg Gln Gln Gly Asn Ile Val Asn Ile Ala 
                405                 410                 415 

ccc cgc gac gag ctg ctt gcc aaa gac aaa gcc ctc tta cag gca gaa     1296 
Pro Arg Asp Glu Leu Leu Ala Lys Asp Lys Ala Leu Leu Gln Ala Glu 
            420                 425                 430 

aaa gac att gcc gat ttg ggt gcg ctg tat tcc caa aac ttc cag ttg     1344 
Lys Asp Ile Ala Asp Leu Gly Ala Leu Tyr Ser Gln Asn Phe Gln Leu 
        435                 440                 445 

aaa tac aaa aat gtg gaa gaa ttc cgc agc atc ctg cgt ttg gac aat     1392 
Lys Tyr Lys Asn Val Glu Glu Phe Arg Ser Ile Leu Arg Leu Asp Asn 
    450                 455                 460 

gcc gac acg acc gga aac cgc aac acg ctt atc agc ggc agg ggc agc     1440 
Ala Asp Thr Thr Gly Asn Arg Asn Thr Leu Ile Ser Gly Arg Gly Ser 
465                 470                 475                 480 

gtg ctg atc gat ccc gcc acc aac acc ctg att gtt acc gac acc cgc     1488 
Val Leu Ile Asp Pro Ala Thr Asn Thr Leu Ile Val Thr Asp Thr Arg 
                485                 490                 495 

agc gtc atc gaa aaa ttc cgc aaa ctg att gac gaa ttg gac gta ccc     1536 
Ser Val Ile Glu Lys Phe Arg Lys Leu Ile Asp Glu Leu Asp Val Pro 
            500                 505                 510 

gcg caa caa gtg atg att gag gcg cgt atc gtc gaa gcg gca gac ggc     1584 
Ala Gln Gln Val Met Ile Glu Ala Arg Ile Val Glu Ala Ala Asp Gly 
        515                 520                 525 

ttc tcg cgc gat ttg ggc gtt aaa ttc ggc gcg aca ggc aag aaa aag     1632 
Phe Ser Arg Asp Leu Gly Val Lys Phe Gly Ala Thr Gly Lys Lys Lys 
    530                 535                 540 

ctg aaa aat gat aca agc gca ttc ggc tgg ggg gta aac tcc ggc ttc     1680 
Leu Lys Asn Asp Thr Ser Ala Phe Gly Trp Gly Val Asn Ser Gly Phe 
545                 550                 555                 560 

ggc ggc gac gat aaa tgg ggg gcc gaa acc aaa atc aac ctg ccg att     1728 
Gly Gly Asp Asp Lys Trp Gly Ala Glu Thr Lys Ile Asn Leu Pro Ile 
                565                 570                 575 

acc gct gcc gca aac agc att tcg ctg gtg cgc gcg att tcc tcc ggt     1776 
Thr Ala Ala Ala Asn Ser Ile Ser Leu Val Arg Ala Ile Ser Ser Gly 
            580                 585                 590 

gcc ttg aat ttg gaa ttg tcc gca tcc gaa tcg ctt tca aaa acc aaa     1824 
Ala Leu Asn Leu Glu Leu Ser Ala Ser Glu Ser Leu Ser Lys Thr Lys 
        595                 600                 605 

acg ctt gcc aat ccg cgc gtg ctg acc caa aac cgc aaa gag gcc aaa     1872 
Thr Leu Ala Asn Pro Arg Val Leu Thr Gln Asn Arg Lys Glu Ala Lys 
    610                 615                 620 

atc gaa tcc ggt tac gaa att cct ttc acc gta acc tca atc gcg aac     1920 
Ile Glu Ser Gly Tyr Glu Ile Pro Phe Thr Val Thr Ser Ile Ala Asn 
625                 630                 635                 640 

ggc ggc agc agc acg aac acg gaa ctc aaa aaa gcc gtc ttg ggg ctg     1968 
Gly Gly Ser Ser Thr Asn Thr Glu Leu Lys Lys Ala Val Leu Gly Leu 
                645                 650                 655 

acc gtt acg ccg aac atc acg ccc gac ggc caa atc att atg acc gtc     2016 
Thr Val Thr Pro Asn Ile Thr Pro Asp Gly Gln Ile Ile Met Thr Val 
            660                 665                 670 

aaa atc aac aag gac tcg cct gcg caa tgt gcc tcc ggt aat cag acg     2064 
Lys Ile Asn Lys Asp Ser Pro Ala Gln Cys Ala Ser Gly Asn Gln Thr 
        675                 680                 685 

atc ctg tgt att tcg acc aaa aac ctg aat acg cag gct atg gtt gaa     2112 
Ile Leu Cys Ile Ser Thr Lys Asn Leu Asn Thr Gln Ala Met Val Glu 
    690                 695                 700 

aac ggc ggc aca ttg att gtc ggc ggt att tat gaa gaa gac aac ggc     2160 
Asn Gly Gly Thr Leu Ile Val Gly Gly Ile Tyr Glu Glu Asp Asn Gly 
705                 710                 715                 720 

aat acg ctg acc aaa gtc ccc ctg ttg ggc gac atc ccc gtt atc ggc     2208 
Asn Thr Leu Thr Lys Val Pro Leu Leu Gly Asp Ile Pro Val Ile Gly 
                725                 730                 735 

aac ctc ttt aaa aca cgc ggg aaa aaa acc gac cgc cgc gaa ctg ctg     2256 
Asn Leu Phe Lys Thr Arg Gly Lys Lys Thr Asp Arg Arg Glu Leu Leu 
            740                 745                 750 

att ttc att acc ccg agg att atg ggt acg gcc ggc aac agc ctg cgc     2304 
Ile Phe Ile Thr Pro Arg Ile Met Gly Thr Ala Gly Asn Ser Leu Arg 
        755                 760                 765 

tat                                                                 2307 
Tyr 

 
           
             10  
             769  
             PRT  
             Neisseria meningitidis  
           
            10 

Met Asn Thr Lys Leu Thr Lys Ile Ile Ser Gly Leu Phe Val Ala Thr 
1               5                   10                  15 

Ala Ala Phe Gln Thr Ala Ser Ala Gly Asn Ile Thr Asp Ile Lys Val 
            20                  25                  30 

Ser Ser Leu Pro Asn Lys Gln Lys Ile Val Lys Val Ser Phe Asp Lys 
        35                  40                  45 

Glu Ile Val Asn Pro Thr Gly Phe Val Thr Ser Ser Pro Ala Arg Ile 
    50                  55                  60 

Ala Leu Asp Phe Glu Gln Thr Gly Ile Ser Met Asp Gln Gln Val Leu 
65                  70                  75                  80 

Glu Tyr Ala Asp Pro Leu Leu Ser Lys Ile Ser Ala Ala Gln Asn Ser 
                85                  90                  95 

Ser Arg Ala Arg Leu Val Leu Asn Leu Asn Lys Pro Gly Gln Tyr Asn 
            100                 105                 110 

Thr Glu Val Arg Gly Asn Lys Val Trp Ile Phe Ile Asn Glu Ser Asp 
        115                 120                 125 

Asp Thr Val Ser Ala Pro Ala Arg Pro Ala Val Lys Ala Ala Pro Ala 
    130                 135                 140 

Ala Pro Ala Lys Gln Gln Ala Ala Ala Pro Ser Thr Lys Ser Ala Val 
145                 150                 155                 160 

Ser Val Ser Glu Pro Phe Thr Pro Ala Lys Gln Gln Ala Ala Ala Pro 
                165                 170                 175 

Phe Thr Glu Ser Val Val Ser Val Ser Ala Pro Phe Ser Pro Ala Lys 
            180                 185                 190 

Gln Gln Ala Ala Ala Ser Ala Lys Gln Gln Ala Ala Ala Pro Ala Lys 
        195                 200                 205 

Gln Gln Ala Ala Ala Pro Ala Lys Gln Gln Ala Ala Ala Pro Ala Lys 
    210                 215                 220 

Gln Thr Asn Ile Asp Phe Arg Lys Asp Gly Lys Asn Ala Gly Ile Ile 
225                 230                 235                 240 

Glu Leu Ala Ala Leu Gly Phe Ala Gly Gln Pro Asp Ile Ser Gln Gln 
                245                 250                 255 

His Asp His Ile Ile Val Thr Leu Lys Asn His Thr Leu Pro Thr Thr 
            260                 265                 270 

Leu Gln Arg Ser Leu Asp Val Ala Asp Phe Lys Thr Pro Val Gln Lys 
        275                 280                 285 

Val Thr Leu Lys Arg Leu Asn Asn Asp Thr Gln Leu Ile Ile Thr Thr 
    290                 295                 300 

Ala Gly Asn Trp Glu Leu Val Asn Lys Ser Ala Ala Pro Gly Tyr Phe 
305                 310                 315                 320 

Thr Phe Gln Val Leu Pro Lys Lys Gln Asn Leu Glu Ser Gly Gly Val 
                325                 330                 335 

Asn Asn Ala Pro Lys Thr Phe Thr Gly Arg Lys Ile Ser Leu Asp Phe 
            340                 345                 350 

Gln Asp Val Glu Ile Arg Thr Ile Leu Gln Ile Leu Ala Lys Glu Ser 
        355                 360                 365 

Gly Met Asn Ile Val Ala Ser Asp Ser Val Asn Gly Lys Met Thr Leu 
    370                 375                 380 

Ser Leu Lys Asp Val Pro Trp Asp Gln Ala Leu Asp Leu Val Met Gln 
385                 390                 395                 400 

Ala Arg Asn Leu Asp Met Arg Gln Gln Gly Asn Ile Val Asn Ile Ala 
                405                 410                 415 

Pro Arg Asp Glu Leu Leu Ala Lys Asp Lys Ala Leu Leu Gln Ala Glu 
            420                 425                 430 

Lys Asp Ile Ala Asp Leu Gly Ala Leu Tyr Ser Gln Asn Phe Gln Leu 
        435                 440                 445 

Lys Tyr Lys Asn Val Glu Glu Phe Arg Ser Ile Leu Arg Leu Asp Asn 
    450                 455                 460 

Ala Asp Thr Thr Gly Asn Arg Asn Thr Leu Ile Ser Gly Arg Gly Ser 
465                 470                 475                 480 

Val Leu Ile Asp Pro Ala Thr Asn Thr Leu Ile Val Thr Asp Thr Arg 
                485                 490                 495 

Ser Val Ile Glu Lys Phe Arg Lys Leu Ile Asp Glu Leu Asp Val Pro 
            500                 505                 510 

Ala Gln Gln Val Met Ile Glu Ala Arg Ile Val Glu Ala Ala Asp Gly 
        515                 520                 525 

Phe Ser Arg Asp Leu Gly Val Lys Phe Gly Ala Thr Gly Lys Lys Lys 
    530                 535                 540 

Leu Lys Asn Asp Thr Ser Ala Phe Gly Trp Gly Val Asn Ser Gly Phe 
545                 550                 555                 560 

Gly Gly Asp Asp Lys Trp Gly Ala Glu Thr Lys Ile Asn Leu Pro Ile 
                565                 570                 575 

Thr Ala Ala Ala Asn Ser Ile Ser Leu Val Arg Ala Ile Ser Ser Gly 
            580                 585                 590 

Ala Leu Asn Leu Glu Leu Ser Ala Ser Glu Ser Leu Ser Lys Thr Lys 
        595                 600                 605 

Thr Leu Ala Asn Pro Arg Val Leu Thr Gln Asn Arg Lys Glu Ala Lys 
    610                 615                 620 

Ile Glu Ser Gly Tyr Glu Ile Pro Phe Thr Val Thr Ser Ile Ala Asn 
625                 630                 635                 640 

Gly Gly Ser Ser Thr Asn Thr Glu Leu Lys Lys Ala Val Leu Gly Leu 
                645                 650                 655 

Thr Val Thr Pro Asn Ile Thr Pro Asp Gly Gln Ile Ile Met Thr Val 
            660                 665                 670 

Lys Ile Asn Lys Asp Ser Pro Ala Gln Cys Ala Ser Gly Asn Gln Thr 
        675                 680                 685 

Ile Leu Cys Ile Ser Thr Lys Asn Leu Asn Thr Gln Ala Met Val Glu 
    690                 695                 700 

Asn Gly Gly Thr Leu Ile Val Gly Gly Ile Tyr Glu Glu Asp Asn Gly 
705                 710                 715                 720 

Asn Thr Leu Thr Lys Val Pro Leu Leu Gly Asp Ile Pro Val Ile Gly 
                725                 730                 735 

Asn Leu Phe Lys Thr Arg Gly Lys Lys Thr Asp Arg Arg Glu Leu Leu 
            740                 745                 750 

Ile Phe Ile Thr Pro Arg Ile Met Gly Thr Ala Gly Asn Ser Leu Arg 
        755                 760                 765 

Tyr 

 
           
             11  
             1773  
             DNA  
             Neisseria meningitidis  
             
               CDS  
               (1)..(1773)  
               Hsf cds  
             
           
            11 

atg aac aaa ata tac cgc atc att tgg aat agt gcc ctc aat gcc tgg       48 
Met Asn Lys Ile Tyr Arg Ile Ile Trp Asn Ser Ala Leu Asn Ala Trp 
1               5                   10                  15 

gtc gtc gta tcc gag ctc aca cgc aac cac acc aaa cgc gcc tcc gca       96 
Val Val Val Ser Glu Leu Thr Arg Asn His Thr Lys Arg Ala Ser Ala 
            20                  25                  30 

acc gtg aag acc gcc gta ttg gcg aca ctg ttg ttt gca acg gtt cag      144 
Thr Val Lys Thr Ala Val Leu Ala Thr Leu Leu Phe Ala Thr Val Gln 
        35                  40                  45 

gca agt gct aac aat gaa gag caa gaa gaa gat tta tat tta gac ccc      192 
Ala Ser Ala Asn Asn Glu Glu Gln Glu Glu Asp Leu Tyr Leu Asp Pro 
    50                  55                  60 

gta caa cgc act gtt gcc gtg ttg ata gtc aat tcc gat aaa gaa ggc      240 
Val Gln Arg Thr Val Ala Val Leu Ile Val Asn Ser Asp Lys Glu Gly 
65                  70                  75                  80 

acg gga gaa aaa gaa aaa gta gaa gaa aat tca gat tgg gca gta tat      288 
Thr Gly Glu Lys Glu Lys Val Glu Glu Asn Ser Asp Trp Ala Val Tyr 
                85                  90                  95 

ttc aac gag aaa gga gta cta aca gcc aga gaa atc acc ctc aaa gcc      336 
Phe Asn Glu Lys Gly Val Leu Thr Ala Arg Glu Ile Thr Leu Lys Ala 
            100                 105                 110 

ggc gac aac ctg aaa atc aaa caa aac ggc aca aac ttc acc tac tcg      384 
Gly Asp Asn Leu Lys Ile Lys Gln Asn Gly Thr Asn Phe Thr Tyr Ser 
        115                 120                 125 

ctg aaa aaa gac ctc aca gat ctg acc agt gtt gga act gaa aaa tta      432 
Leu Lys Lys Asp Leu Thr Asp Leu Thr Ser Val Gly Thr Glu Lys Leu 
    130                 135                 140 

tcg ttt agc gca aac ggc aat aaa gtc aac atc aca agc gac acc aaa      480 
Ser Phe Ser Ala Asn Gly Asn Lys Val Asn Ile Thr Ser Asp Thr Lys 
145                 150                 155                 160 

ggc ttg aat ttt gcg aaa gaa acg gct ggg acg aac ggc gac acc acg      528 
Gly Leu Asn Phe Ala Lys Glu Thr Ala Gly Thr Asn Gly Asp Thr Thr 
                165                 170                 175 

gtt cat ctg aac ggt att ggt tcg act ttg acc gat acg ctg ctg aat      576 
Val His Leu Asn Gly Ile Gly Ser Thr Leu Thr Asp Thr Leu Leu Asn 
            180                 185                 190 

acc gga gcg acc aca aac gta acc aac gac aac gtt acc gat gac gag      624 
Thr Gly Ala Thr Thr Asn Val Thr Asn Asp Asn Val Thr Asp Asp Glu 
        195                 200                 205 

aaa aaa cgt gcg gca agc gtt aaa gac gta tta aac gct ggc tgg aac      672 
Lys Lys Arg Ala Ala Ser Val Lys Asp Val Leu Asn Ala Gly Trp Asn 
    210                 215                 220 

att aaa ggc gtt aaa ccc ggt aca aca gct tcc gat aac gtt gat ttc      720 
Ile Lys Gly Val Lys Pro Gly Thr Thr Ala Ser Asp Asn Val Asp Phe 
225                 230                 235                 240 

gtc cgc act tac gac aca gtc gag ttc ttg agc gca gat acg aaa aca      768 
Val Arg Thr Tyr Asp Thr Val Glu Phe Leu Ser Ala Asp Thr Lys Thr 
                245                 250                 255 

acg act gtt aat gtg gaa agc aaa gac aac ggc aag aaa acc gaa gtt      816 
Thr Thr Val Asn Val Glu Ser Lys Asp Asn Gly Lys Lys Thr Glu Val 
            260                 265                 270 

aaa atc ggt gcg aag act tct gtt att aaa gaa aaa gac ggt aag ttg      864 
Lys Ile Gly Ala Lys Thr Ser Val Ile Lys Glu Lys Asp Gly Lys Leu 
        275                 280                 285 

gtt act ggt aaa gac aaa ggc gag aat ggt tct tct aca gac gaa ggc      912 
Val Thr Gly Lys Asp Lys Gly Glu Asn Gly Ser Ser Thr Asp Glu Gly 
    290                 295                 300 

gaa ggc tta gtg act gca aaa gaa gtg att gat gca gta aac aag gct      960 
Glu Gly Leu Val Thr Ala Lys Glu Val Ile Asp Ala Val Asn Lys Ala 
305                 310                 315                 320 

ggt tgg aga atg aaa aca aca acc gct aat ggt caa aca ggt caa gct     1008 
Gly Trp Arg Met Lys Thr Thr Thr Ala Asn Gly Gln Thr Gly Gln Ala 
                325                 330                 335 

gac aag ttt gaa acc gtt aca tca ggc aca aat gta acc ttt gct agt     1056 
Asp Lys Phe Glu Thr Val Thr Ser Gly Thr Asn Val Thr Phe Ala Ser 
            340                 345                 350 

ggt aaa ggt aca act gcg act gta agt aaa gat gat caa ggc aac atc     1104 
Gly Lys Gly Thr Thr Ala Thr Val Ser Lys Asp Asp Gln Gly Asn Ile 
        355                 360                 365 

act gtt atg tat gat gta aat gtc ggc gat gcc cta aac gtc aat cag     1152 
Thr Val Met Tyr Asp Val Asn Val Gly Asp Ala Leu Asn Val Asn Gln 
    370                 375                 380 

ctg caa aac agc ggt tgg aat ttg gat tcc aaa gcg gtt gca ggt tct     1200 
Leu Gln Asn Ser Gly Trp Asn Leu Asp Ser Lys Ala Val Ala Gly Ser 
385                 390                 395                 400 

tcg ggc aaa gtc atc agc ggc aat gtt tcg ccg agc aag gga aag atg     1248 
Ser Gly Lys Val Ile Ser Gly Asn Val Ser Pro Ser Lys Gly Lys Met 
                405                 410                 415 

gat gaa acc gtc aac att aat gcc ggc aac aac atc gag att acc cgc     1296 
Asp Glu Thr Val Asn Ile Asn Ala Gly Asn Asn Ile Glu Ile Thr Arg 
            420                 425                 430 

aac ggt aaa aat atc gac atc gcc act tcg atg acc ccg cag ttt tcc     1344 
Asn Gly Lys Asn Ile Asp Ile Ala Thr Ser Met Thr Pro Gln Phe Ser 
        435                 440                 445 

agc gtt tcg ctc ggc gcg ggg gcg gat gcg ccc act ttg agc gtg gat     1392 
Ser Val Ser Leu Gly Ala Gly Ala Asp Ala Pro Thr Leu Ser Val Asp 
    450                 455                 460 

ggg gac gca ttg aat gtc ggc agc aag aag gac aac aaa ccc gtc cgc     1440 
Gly Asp Ala Leu Asn Val Gly Ser Lys Lys Asp Asn Lys Pro Val Arg 
465                 470                 475                 480 

att acc aat gtc gcc ccg ggc gtt aaa gag ggg gat gtt aca aac gtc     1488 
Ile Thr Asn Val Ala Pro Gly Val Lys Glu Gly Asp Val Thr Asn Val 
                485                 490                 495 

gca caa ctt aaa ggc gtg gcg caa aac ttg aac aac cgc atc gac aat     1536 
Ala Gln Leu Lys Gly Val Ala Gln Asn Leu Asn Asn Arg Ile Asp Asn 
            500                 505                 510 

gtg gac ggc aac gcg cgt gcg ggc atc gcc caa gcg att gca acc gca     1584 
Val Asp Gly Asn Ala Arg Ala Gly Ile Ala Gln Ala Ile Ala Thr Ala 
        515                 520                 525 

ggt ctg gtt cag gcg tat ttg ccc ggc aag agt atg atg gcg atc ggc     1632 
Gly Leu Val Gln Ala Tyr Leu Pro Gly Lys Ser Met Met Ala Ile Gly 
    530                 535                 540 

ggc ggc act tat cgc ggc gaa gcc ggt tac gcc atc ggc tac tcc agt     1680 
Gly Gly Thr Tyr Arg Gly Glu Ala Gly Tyr Ala Ile Gly Tyr Ser Ser 
545                 550                 555                 560 

att tcc gac ggc gga aat tgg att atc aaa ggc acg gct tcc ggc aat     1728 
Ile Ser Asp Gly Gly Asn Trp Ile Ile Lys Gly Thr Ala Ser Gly Asn 
                565                 570                 575 

tcg cgc ggc cat ttc ggt gct tcc gca tct gtc ggt tat cag tgg         1773 
Ser Arg Gly His Phe Gly Ala Ser Ala Ser Val Gly Tyr Gln Trp 
            580                 585                 590 

 
           
             12  
             591  
             PRT  
             Neisseria meningitidis  
           
            12 

Met Asn Lys Ile Tyr Arg Ile Ile Trp Asn Ser Ala Leu Asn Ala Trp 
1               5                   10                  15 

Val Val Val Ser Glu Leu Thr Arg Asn His Thr Lys Arg Ala Ser Ala 
            20                  25                  30 

Thr Val Lys Thr Ala Val Leu Ala Thr Leu Leu Phe Ala Thr Val Gln 
        35                  40                  45 

Ala Ser Ala Asn Asn Glu Glu Gln Glu Glu Asp Leu Tyr Leu Asp Pro 
    50                  55                  60 

Val Gln Arg Thr Val Ala Val Leu Ile Val Asn Ser Asp Lys Glu Gly 
65                  70                  75                  80 

Thr Gly Glu Lys Glu Lys Val Glu Glu Asn Ser Asp Trp Ala Val Tyr 
                85                  90                  95 

Phe Asn Glu Lys Gly Val Leu Thr Ala Arg Glu Ile Thr Leu Lys Ala 
            100                 105                 110 

Gly Asp Asn Leu Lys Ile Lys Gln Asn Gly Thr Asn Phe Thr Tyr Ser 
        115                 120                 125 

Leu Lys Lys Asp Leu Thr Asp Leu Thr Ser Val Gly Thr Glu Lys Leu 
    130                 135                 140 

Ser Phe Ser Ala Asn Gly Asn Lys Val Asn Ile Thr Ser Asp Thr Lys 
145                 150                 155                 160 

Gly Leu Asn Phe Ala Lys Glu Thr Ala Gly Thr Asn Gly Asp Thr Thr 
                165                 170                 175 

Val His Leu Asn Gly Ile Gly Ser Thr Leu Thr Asp Thr Leu Leu Asn 
            180                 185                 190 

Thr Gly Ala Thr Thr Asn Val Thr Asn Asp Asn Val Thr Asp Asp Glu 
        195                 200                 205 

Lys Lys Arg Ala Ala Ser Val Lys Asp Val Leu Asn Ala Gly Trp Asn 
    210                 215                 220 

Ile Lys Gly Val Lys Pro Gly Thr Thr Ala Ser Asp Asn Val Asp Phe 
225                 230                 235                 240 

Val Arg Thr Tyr Asp Thr Val Glu Phe Leu Ser Ala Asp Thr Lys Thr 
                245                 250                 255 

Thr Thr Val Asn Val Glu Ser Lys Asp Asn Gly Lys Lys Thr Glu Val 
            260                 265                 270 

Lys Ile Gly Ala Lys Thr Ser Val Ile Lys Glu Lys Asp Gly Lys Leu 
        275                 280                 285 

Val Thr Gly Lys Asp Lys Gly Glu Asn Gly Ser Ser Thr Asp Glu Gly 
    290                 295                 300 

Glu Gly Leu Val Thr Ala Lys Glu Val Ile Asp Ala Val Asn Lys Ala 
305                 310                 315                 320 

Gly Trp Arg Met Lys Thr Thr Thr Ala Asn Gly Gln Thr Gly Gln Ala 
                325                 330                 335 

Asp Lys Phe Glu Thr Val Thr Ser Gly Thr Asn Val Thr Phe Ala Ser 
            340                 345                 350 

Gly Lys Gly Thr Thr Ala Thr Val Ser Lys Asp Asp Gln Gly Asn Ile 
        355                 360                 365 

Thr Val Met Tyr Asp Val Asn Val Gly Asp Ala Leu Asn Val Asn Gln 
    370                 375                 380 

Leu Gln Asn Ser Gly Trp Asn Leu Asp Ser Lys Ala Val Ala Gly Ser 
385                 390                 395                 400 

Ser Gly Lys Val Ile Ser Gly Asn Val Ser Pro Ser Lys Gly Lys Met 
                405                 410                 415 

Asp Glu Thr Val Asn Ile Asn Ala Gly Asn Asn Ile Glu Ile Thr Arg 
            420                 425                 430 

Asn Gly Lys Asn Ile Asp Ile Ala Thr Ser Met Thr Pro Gln Phe Ser 
        435                 440                 445 

Ser Val Ser Leu Gly Ala Gly Ala Asp Ala Pro Thr Leu Ser Val Asp 
    450                 455                 460 

Gly Asp Ala Leu Asn Val Gly Ser Lys Lys Asp Asn Lys Pro Val Arg 
465                 470                 475                 480 

Ile Thr Asn Val Ala Pro Gly Val Lys Glu Gly Asp Val Thr Asn Val 
                485                 490                 495 

Ala Gln Leu Lys Gly Val Ala Gln Asn Leu Asn Asn Arg Ile Asp Asn 
            500                 505                 510 

Val Asp Gly Asn Ala Arg Ala Gly Ile Ala Gln Ala Ile Ala Thr Ala 
        515                 520                 525 

Gly Leu Val Gln Ala Tyr Leu Pro Gly Lys Ser Met Met Ala Ile Gly 
    530                 535                 540 

Gly Gly Thr Tyr Arg Gly Glu Ala Gly Tyr Ala Ile Gly Tyr Ser Ser 
545                 550                 555                 560 

Ile Ser Asp Gly Gly Asn Trp Ile Ile Lys Gly Thr Ala Ser Gly Asn 
                565                 570                 575 

Ser Arg Gly His Phe Gly Ala Ser Ala Ser Val Gly Tyr Gln Trp 
            580                 585                 590 

 
           
             13  
             549  
             DNA  
             Neisseria meningitidis  
             
               CDS  
               (1)..(549)  
               NMA0174  
             
           
            13 

atg aaa gtt ttg aac ggt tgg tcg gac agg aag atg tgg cgg gtt ttg       48 
Met Lys Val Leu Asn Gly Trp Ser Asp Arg Lys Met Trp Arg Val Leu 
1               5                   10                  15 

agt gct ttg ccg ata ggc gtg gtg ttt ttt gat ttg atc tac ggt ttt       96 
Ser Ala Leu Pro Ile Gly Val Val Phe Phe Asp Leu Ile Tyr Gly Phe 
            20                  25                  30 

gtg ttg aat gtg ttg cag ggt ttg gat ttg cag cgt gcc gtg ccg gat      144 
Val Leu Asn Val Leu Gln Gly Leu Asp Leu Gln Arg Ala Val Pro Asp 
        35                  40                  45 

tcg gaa ggc gtg ttg gcg gtt acg ccc gat att gca ttc aac agt ttg      192 
Ser Glu Gly Val Leu Ala Val Thr Pro Asp Ile Ala Phe Asn Ser Leu 
    50                  55                  60 

cag att gtc gcc aac ggc ggt atg gcg gcg gtg gtc tgt ttc ggg ttg      240 
Gln Ile Val Ala Asn Gly Gly Met Ala Ala Val Val Cys Phe Gly Leu 
65                  70                  75                  80 

gcg gtt gtg ttt ttg ctc aac cgt tcg gtg cgg cgg cgg cag gtg ttg      288 
Ala Val Val Phe Leu Leu Asn Arg Ser Val Arg Arg Arg Gln Val Leu 
                85                  90                  95 

gaa atc ggg gtg ttc cgg atg ttg ggg ctg gtg gcg gta ttg gcg ttc      336 
Glu Ile Gly Val Phe Arg Met Leu Gly Leu Val Ala Val Leu Ala Phe 
            100                 105                 110 

agc gcg ccg tcg gtg tgg gag tgg gcg aac gcg ctg ccg ctg ctg ctg      384 
Ser Ala Pro Ser Val Trp Glu Trp Ala Asn Ala Leu Pro Leu Leu Leu 
        115                 120                 125 

aag ggc gcg gac gtg gtc aat acg ggg aat gcg cgt tat gtg ctg acg      432 
Lys Gly Ala Asp Val Val Asn Thr Gly Asn Ala Arg Tyr Val Leu Thr 
    130                 135                 140 

gct ttg tgt atg ccc ttt cct gcg gtg tcg tgc gtc atc ggg ctg gtg      480 
Ala Leu Cys Met Pro Phe Pro Ala Val Ser Cys Val Ile Gly Leu Val 
145                 150                 155                 160 

ggg cgg ttc agg ctt cag acg gca tcg ggc agg gcg gca aag tca ggg      528 
Gly Arg Phe Arg Leu Gln Thr Ala Ser Gly Arg Ala Ala Lys Ser Gly 
                165                 170                 175 

ggt gcg ggc aag gcg gac gga                                          549 
Gly Ala Gly Lys Ala Asp Gly 
            180 

 
           
             14  
             183  
             PRT  
             Neisseria meningitidis  
           
            14 

Met Lys Val Leu Asn Gly Trp Ser Asp Arg Lys Met Trp Arg Val Leu 
1               5                   10                  15 

Ser Ala Leu Pro Ile Gly Val Val Phe Phe Asp Leu Ile Tyr Gly Phe 
            20                  25                  30 

Val Leu Asn Val Leu Gln Gly Leu Asp Leu Gln Arg Ala Val Pro Asp 
        35                  40                  45 

Ser Glu Gly Val Leu Ala Val Thr Pro Asp Ile Ala Phe Asn Ser Leu 
    50                  55                  60 

Gln Ile Val Ala Asn Gly Gly Met Ala Ala Val Val Cys Phe Gly Leu 
65                  70                  75                  80 

Ala Val Val Phe Leu Leu Asn Arg Ser Val Arg Arg Arg Gln Val Leu 
                85                  90                  95 

Glu Ile Gly Val Phe Arg Met Leu Gly Leu Val Ala Val Leu Ala Phe 
            100                 105                 110 

Ser Ala Pro Ser Val Trp Glu Trp Ala Asn Ala Leu Pro Leu Leu Leu 
        115                 120                 125 

Lys Gly Ala Asp Val Val Asn Thr Gly Asn Ala Arg Tyr Val Leu Thr 
    130                 135                 140 

Ala Leu Cys Met Pro Phe Pro Ala Val Ser Cys Val Ile Gly Leu Val 
145                 150                 155                 160 

Gly Arg Phe Arg Leu Gln Thr Ala Ser Gly Arg Ala Ala Lys Ser Gly 
                165                 170                 175 

Gly Ala Gly Lys Ala Asp Gly 
            180 

 
           
             15  
             2373  
             DNA  
             Neisseria meningitidis  
             
               CDS  
               (1)..(2373)  
               NMA1371  
             
           
            15 

atg aat aaa aat att aaa tct tta aat tta cgg gaa aaa gac ccg ttt       48 
Met Asn Lys Asn Ile Lys Ser Leu Asn Leu Arg Glu Lys Asp Pro Phe 
1               5                   10                  15 

tta agt cgt gaa aaa cag cgt tat gaa cat cct ttg ccc agt cgg gaa       96 
Leu Ser Arg Glu Lys Gln Arg Tyr Glu His Pro Leu Pro Ser Arg Glu 
            20                  25                  30 

tgg ata atc gag ctg ctt gaa cgt aaa ggc gta cca tcc aag att gaa      144 
Trp Ile Ile Glu Leu Leu Glu Arg Lys Gly Val Pro Ser Lys Ile Glu 
        35                  40                  45 

gct ttg gta cgc gaa ttg tcg att aag gaa gaa gag tac gaa ttt ttc      192 
Ala Leu Val Arg Glu Leu Ser Ile Lys Glu Glu Glu Tyr Glu Phe Phe 
    50                  55                  60 

gaa cgt cgt ctg aag gcg atg gcg cgg gac ggt cag gtt tta atc aac      240 
Glu Arg Arg Leu Lys Ala Met Ala Arg Asp Gly Gln Val Leu Ile Asn 
65                  70                  75                  80 

cgt cgg ggc gcg gtt tgc gcg gcg gac aaa ttg gat ttg gtc aaa tgc      288 
Arg Arg Gly Ala Val Cys Ala Ala Asp Lys Leu Asp Leu Val Lys Cys 
                85                  90                  95 

cgt gtc gag gcg cac aaa gac ggc ttc ggt ttc gcc gtg ccg ctc acg      336 
Arg Val Glu Ala His Lys Asp Gly Phe Gly Phe Ala Val Pro Leu Thr 
            100                 105                 110 

ccc gcc aaa gac ggt gat ttt gtt ttg tac gaa cgc cag atg cgc ggc      384 
Pro Ala Lys Asp Gly Asp Phe Val Leu Tyr Glu Arg Gln Met Arg Gly 
        115                 120                 125 

att atg cac ggc gat att gtc act gtt cgt cct gcc ggc atg gac cgt      432 
Ile Met His Gly Asp Ile Val Thr Val Arg Pro Ala Gly Met Asp Arg 
    130                 135                 140 

agg ggc cgc cgc gaa ggg acg gtt ctg gat att gtc gaa cgc gcg caa      480 
Arg Gly Arg Arg Glu Gly Thr Val Leu Asp Ile Val Glu Arg Ala Gln 
145                 150                 155                 160 

agc aaa gtg gtc ggc cgt ttc tat atg gat agg ggc gtg gcg att ttg      528 
Ser Lys Val Val Gly Arg Phe Tyr Met Asp Arg Gly Val Ala Ile Leu 
                165                 170                 175 

gag ccg gaa gac aag cgt ctg aac caa agc atc gta ttg gaa ccg gac      576 
Glu Pro Glu Asp Lys Arg Leu Asn Gln Ser Ile Val Leu Glu Pro Asp 
            180                 185                 190 

ggc gtg gcg cgt ttc aaa cct gaa tcc ggt cag gtc atc gtc ggc gaa      624 
Gly Val Ala Arg Phe Lys Pro Glu Ser Gly Gln Val Ile Val Gly Glu 
        195                 200                 205 

att gag gtt tat cct gag caa aac cgg ccg gca gtg gca aaa atc atc      672 
Ile Glu Val Tyr Pro Glu Gln Asn Arg Pro Ala Val Ala Lys Ile Ile 
    210                 215                 220 

gaa gtt ttg ggc gat tat gcc gac agc ggc atg gag att gaa att gcc      720 
Glu Val Leu Gly Asp Tyr Ala Asp Ser Gly Met Glu Ile Glu Ile Ala 
225                 230                 235                 240 

gtg cgc aag cat cat ttg ccg cac caa ttc agt gaa gcg tgt gcc aaa      768 
Val Arg Lys His His Leu Pro His Gln Phe Ser Glu Ala Cys Ala Lys 
                245                 250                 255 

gcc gcg aaa aaa att ccc gac cat gta cgc aaa agc gat ttg aaa ggc      816 
Ala Ala Lys Lys Ile Pro Asp His Val Arg Lys Ser Asp Leu Lys Gly 
            260                 265                 270 

cgc gtc gat ttg cgc gac ctg cct ttg gta acg ata gac ggc gaa acg      864 
Arg Val Asp Leu Arg Asp Leu Pro Leu Val Thr Ile Asp Gly Glu Thr 
        275                 280                 285 

gct cga gat ttt gac gat gcg gtg ttt gcc gag aaa atc gga cgc aat      912 
Ala Arg Asp Phe Asp Asp Ala Val Phe Ala Glu Lys Ile Gly Arg Asn 
    290                 295                 300 

tac cgt ctg gtc gtg gcg att gcc gat gtc agc cat tat gtc cgc ccc      960 
Tyr Arg Leu Val Val Ala Ile Ala Asp Val Ser His Tyr Val Arg Pro 
305                 310                 315                 320 

gat gac gct atc gac acg gac gct cag gaa cgc agc acc agt gtt tac     1008 
Asp Asp Ala Ile Asp Thr Asp Ala Gln Glu Arg Ser Thr Ser Val Tyr 
                325                 330                 335 

ttc ccg cgc cgc gtg att ccc atg ttg ccg gaa aac ctg tcc aac ggc     1056 
Phe Pro Arg Arg Val Ile Pro Met Leu Pro Glu Asn Leu Ser Asn Gly 
            340                 345                 350 

atc tgc tcg ctc aat cct cat gtc gag cgt ttg tgt gtg gtg tgc gat     1104 
Ile Cys Ser Leu Asn Pro His Val Glu Arg Leu Cys Val Val Cys Asp 
        355                 360                 365 

atg gtt atc act tac gcg ggc aat atc aaa gaa tac cgc ttc tac ccc     1152 
Met Val Ile Thr Tyr Ala Gly Asn Ile Lys Glu Tyr Arg Phe Tyr Pro 
    370                 375                 380 

gcc gtg atg cgc tct cat gcc cgc ctg acc tac aac caa gtt tgg aaa     1200 
Ala Val Met Arg Ser His Ala Arg Leu Thr Tyr Asn Gln Val Trp Lys 
385                 390                 395                 400 

tgg ctt tca ggc ggc atc gag cat ccg ttc aaa acc caa atc gac acg     1248 
Trp Leu Ser Gly Gly Ile Glu His Pro Phe Lys Thr Gln Ile Asp Thr 
                405                 410                 415 

ctt tac aaa ctc ttc aaa atc ctt cag aaa aag cgt ttc gaa cgc ggg     1296 
Leu Tyr Lys Leu Phe Lys Ile Leu Gln Lys Lys Arg Phe Glu Arg Gly 
            420                 425                 430 

gcg gtg gag ttt gac agc atc gaa acc caa atg ctt ttc gac gac aac     1344 
Ala Val Glu Phe Asp Ser Ile Glu Thr Gln Met Leu Phe Asp Asp Asn 
        435                 440                 445 

ggt aaa att gaa aaa atc gtc ccc gtt gtc cgc aac gat gcc cac aag     1392 
Gly Lys Ile Glu Lys Ile Val Pro Val Val Arg Asn Asp Ala His Lys 
    450                 455                 460 

ctg att gaa gaa tgt atg ttg gcg gca aac gtt tgc gca gcg gat ttt     1440 
Leu Ile Glu Glu Cys Met Leu Ala Ala Asn Val Cys Ala Ala Asp Phe 
465                 470                 475                 480 

ctg ttg aaa aac aag cat acc gca ttg ttc cgc aac cat ttg ggg ccc     1488 
Leu Leu Lys Asn Lys His Thr Ala Leu Phe Arg Asn His Leu Gly Pro 
                485                 490                 495 

acg ccc gaa aaa ctc gcc gcc ttg cgc gag cag ctc ggt ctg ttg ggg     1536 
Thr Pro Glu Lys Leu Ala Ala Leu Arg Glu Gln Leu Gly Leu Leu Gly 
            500                 505                 510 

ctt caa ctt ggc ggc ggc gac aac ccg tcg ccg aaa gac tat gcc gcg     1584 
Leu Gln Leu Gly Gly Gly Asp Asn Pro Ser Pro Lys Asp Tyr Ala Ala 
        515                 520                 525 

ctt gcc gga cag ttc aaa ggc agg ccg gat gcc gaa ttg ctg caa gtc     1632 
Leu Ala Gly Gln Phe Lys Gly Arg Pro Asp Ala Glu Leu Leu Gln Val 
    530                 535                 540 

atg atg ttg cgc tcc atg caa cag gcg gtt tac gaa ccg cat tgc gac     1680 
Met Met Leu Arg Ser Met Gln Gln Ala Val Tyr Glu Pro His Cys Asp 
545                 550                 555                 560 

gga cac ttt ggt ctt gcc tac gaa gca tac gcc cac ttc acc tcg ccc     1728 
Gly His Phe Gly Leu Ala Tyr Glu Ala Tyr Ala His Phe Thr Ser Pro 
                565                 570                 575 

atc cgc cgc tat ccc gac ctg acc gta cac cgc gcc atc aaa gcc gtg     1776 
Ile Arg Arg Tyr Pro Asp Leu Thr Val His Arg Ala Ile Lys Ala Val 
            580                 585                 590 

ttg aat cag caa acc tac acg cca aaa aaa agc tgg cag gct ttg ggc     1824 
Leu Asn Gln Gln Thr Tyr Thr Pro Lys Lys Ser Trp Gln Ala Leu Gly 
        595                 600                 605 

gtg cat acc tcg ttc tgt gag cgc cgt gcc gac gac gcc agc cgc gac     1872 
Val His Thr Ser Phe Cys Glu Arg Arg Ala Asp Asp Ala Ser Arg Asp 
    610                 615                 620 

gtg gaa aac tgg ctg aaa acc tat tat atg cgc gat aag gtc ggc gaa     1920 
Val Glu Asn Trp Leu Lys Thr Tyr Tyr Met Arg Asp Lys Val Gly Glu 
625                 630                 635                 640 

gta ttc gaa ggt aaa atc tcc ggc atg acc agt ttt ggt atc ttt gta     1968 
Val Phe Glu Gly Lys Ile Ser Gly Met Thr Ser Phe Gly Ile Phe Val 
                645                 650                 655 

aca ctg gac ggc atc cac att gac ggc ttg gtg cat atc agc gat ttg     2016 
Thr Leu Asp Gly Ile His Ile Asp Gly Leu Val His Ile Ser Asp Leu 
            660                 665                 670 

ggc gaa gac tat ttc aac ttc cgc ccc gaa atc atg gca atc gaa ggc     2064 
Gly Glu Asp Tyr Phe Asn Phe Arg Pro Glu Ile Met Ala Ile Glu Gly 
        675                 680                 685 

gaa cgc agc ggc atc cgt ttc aac atg ggg gac agg gtt gcc gtc cgg     2112 
Glu Arg Ser Gly Ile Arg Phe Asn Met Gly Asp Arg Val Ala Val Arg 
    690                 695                 700 

gtc gcc cgt gcc gat ttg gat gac gga aaa atc gat ttt gtc ctg att     2160 
Val Ala Arg Ala Asp Leu Asp Asp Gly Lys Ile Asp Phe Val Leu Ile 
705                 710                 715                 720 

gcc ggg ggg agc ggc agg ggg cgg aaa gtt aaa tca tcc gcg tct gcc     2208 
Ala Gly Gly Ser Gly Arg Gly Arg Lys Val Lys Ser Ser Ala Ser Ala 
                725                 730                 735 

aaa ccg gca ggg acg gcg ggg aaa ggg aag ccg aaa acc gcc gcc gag     2256 
Lys Pro Ala Gly Thr Ala Gly Lys Gly Lys Pro Lys Thr Ala Ala Glu 
            740                 745                 750 

aaa aaa aca gcc cga ggc ggc aaa gta agg gga agg ggc gcg tct gcc     2304 
Lys Lys Thr Ala Arg Gly Gly Lys Val Arg Gly Arg Gly Ala Ser Ala 
        755                 760                 765 

gcc gca gaa tcg agg aaa aag gca aag aaa ccg gtt ccg att aag gta     2352 
Ala Ala Glu Ser Arg Lys Lys Ala Lys Lys Pro Val Pro Ile Lys Val 
    770                 775                 780 

aaa aaa cgg aaa ggc aaa tca                                         2373 
Lys Lys Arg Lys Gly Lys Ser 
785                 790 

 
           
             16  
             791  
             PRT  
             Neisseria meningitidis  
           
            16 

Met Asn Lys Asn Ile Lys Ser Leu Asn Leu Arg Glu Lys Asp Pro Phe 
1               5                   10                  15 

Leu Ser Arg Glu Lys Gln Arg Tyr Glu His Pro Leu Pro Ser Arg Glu 
            20                  25                  30 

Trp Ile Ile Glu Leu Leu Glu Arg Lys Gly Val Pro Ser Lys Ile Glu 
        35                  40                  45 

Ala Leu Val Arg Glu Leu Ser Ile Lys Glu Glu Glu Tyr Glu Phe Phe 
    50                  55                  60 

Glu Arg Arg Leu Lys Ala Met Ala Arg Asp Gly Gln Val Leu Ile Asn 
65                  70                  75                  80 

Arg Arg Gly Ala Val Cys Ala Ala Asp Lys Leu Asp Leu Val Lys Cys 
                85                  90                  95 

Arg Val Glu Ala His Lys Asp Gly Phe Gly Phe Ala Val Pro Leu Thr 
            100                 105                 110 

Pro Ala Lys Asp Gly Asp Phe Val Leu Tyr Glu Arg Gln Met Arg Gly 
        115                 120                 125 

Ile Met His Gly Asp Ile Val Thr Val Arg Pro Ala Gly Met Asp Arg 
    130                 135                 140 

Arg Gly Arg Arg Glu Gly Thr Val Leu Asp Ile Val Glu Arg Ala Gln 
145                 150                 155                 160 

Ser Lys Val Val Gly Arg Phe Tyr Met Asp Arg Gly Val Ala Ile Leu 
                165                 170                 175 

Glu Pro Glu Asp Lys Arg Leu Asn Gln Ser Ile Val Leu Glu Pro Asp 
            180                 185                 190 

Gly Val Ala Arg Phe Lys Pro Glu Ser Gly Gln Val Ile Val Gly Glu 
        195                 200                 205 

Ile Glu Val Tyr Pro Glu Gln Asn Arg Pro Ala Val Ala Lys Ile Ile 
    210                 215                 220 

Glu Val Leu Gly Asp Tyr Ala Asp Ser Gly Met Glu Ile Glu Ile Ala 
225                 230                 235                 240 

Val Arg Lys His His Leu Pro His Gln Phe Ser Glu Ala Cys Ala Lys 
                245                 250                 255 

Ala Ala Lys Lys Ile Pro Asp His Val Arg Lys Ser Asp Leu Lys Gly 
            260                 265                 270 

Arg Val Asp Leu Arg Asp Leu Pro Leu Val Thr Ile Asp Gly Glu Thr 
        275                 280                 285 

Ala Arg Asp Phe Asp Asp Ala Val Phe Ala Glu Lys Ile Gly Arg Asn 
    290                 295                 300 

Tyr Arg Leu Val Val Ala Ile Ala Asp Val Ser His Tyr Val Arg Pro 
305                 310                 315                 320 

Asp Asp Ala Ile Asp Thr Asp Ala Gln Glu Arg Ser Thr Ser Val Tyr 
                325                 330                 335 

Phe Pro Arg Arg Val Ile Pro Met Leu Pro Glu Asn Leu Ser Asn Gly 
            340                 345                 350 

Ile Cys Ser Leu Asn Pro His Val Glu Arg Leu Cys Val Val Cys Asp 
        355                 360                 365 

Met Val Ile Thr Tyr Ala Gly Asn Ile Lys Glu Tyr Arg Phe Tyr Pro 
    370                 375                 380 

Ala Val Met Arg Ser His Ala Arg Leu Thr Tyr Asn Gln Val Trp Lys 
385                 390                 395                 400 

Trp Leu Ser Gly Gly Ile Glu His Pro Phe Lys Thr Gln Ile Asp Thr 
                405                 410                 415 

Leu Tyr Lys Leu Phe Lys Ile Leu Gln Lys Lys Arg Phe Glu Arg Gly 
            420                 425                 430 

Ala Val Glu Phe Asp Ser Ile Glu Thr Gln Met Leu Phe Asp Asp Asn 
        435                 440                 445 

Gly Lys Ile Glu Lys Ile Val Pro Val Val Arg Asn Asp Ala His Lys 
    450                 455                 460 

Leu Ile Glu Glu Cys Met Leu Ala Ala Asn Val Cys Ala Ala Asp Phe 
465                 470                 475                 480 

Leu Leu Lys Asn Lys His Thr Ala Leu Phe Arg Asn His Leu Gly Pro 
                485                 490                 495 

Thr Pro Glu Lys Leu Ala Ala Leu Arg Glu Gln Leu Gly Leu Leu Gly 
            500                 505                 510 

Leu Gln Leu Gly Gly Gly Asp Asn Pro Ser Pro Lys Asp Tyr Ala Ala 
        515                 520                 525 

Leu Ala Gly Gln Phe Lys Gly Arg Pro Asp Ala Glu Leu Leu Gln Val 
    530                 535                 540 

Met Met Leu Arg Ser Met Gln Gln Ala Val Tyr Glu Pro His Cys Asp 
545                 550                 555                 560 

Gly His Phe Gly Leu Ala Tyr Glu Ala Tyr Ala His Phe Thr Ser Pro 
                565                 570                 575 

Ile Arg Arg Tyr Pro Asp Leu Thr Val His Arg Ala Ile Lys Ala Val 
            580                 585                 590 

Leu Asn Gln Gln Thr Tyr Thr Pro Lys Lys Ser Trp Gln Ala Leu Gly 
        595                 600                 605 

Val His Thr Ser Phe Cys Glu Arg Arg Ala Asp Asp Ala Ser Arg Asp 
    610                 615                 620 

Val Glu Asn Trp Leu Lys Thr Tyr Tyr Met Arg Asp Lys Val Gly Glu 
625                 630                 635                 640 

Val Phe Glu Gly Lys Ile Ser Gly Met Thr Ser Phe Gly Ile Phe Val 
                645                 650                 655 

Thr Leu Asp Gly Ile His Ile Asp Gly Leu Val His Ile Ser Asp Leu 
            660                 665                 670 

Gly Glu Asp Tyr Phe Asn Phe Arg Pro Glu Ile Met Ala Ile Glu Gly 
        675                 680                 685 

Glu Arg Ser Gly Ile Arg Phe Asn Met Gly Asp Arg Val Ala Val Arg 
    690                 695                 700 

Val Ala Arg Ala Asp Leu Asp Asp Gly Lys Ile Asp Phe Val Leu Ile 
705                 710                 715                 720 

Ala Gly Gly Ser Gly Arg Gly Arg Lys Val Lys Ser Ser Ala Ser Ala 
                725                 730                 735 

Lys Pro Ala Gly Thr Ala Gly Lys Gly Lys Pro Lys Thr Ala Ala Glu 
            740                 745                 750 

Lys Lys Thr Ala Arg Gly Gly Lys Val Arg Gly Arg Gly Ala Ser Ala 
        755                 760                 765 

Ala Ala Glu Ser Arg Lys Lys Ala Lys Lys Pro Val Pro Ile Lys Val 
    770                 775                 780 

Lys Lys Arg Lys Gly Lys Ser 
785                 790 

 
           
             17  
             1605  
             DNA  
             Neisseria meningitidis  
             
               CDS  
               (1)..(1605)  
               NMA1555  
             
           
            17 

atg agt atc gta gaa atc aaa gtc ccc gat atc ggc ggt cac gaa aac       48 
Met Ser Ile Val Glu Ile Lys Val Pro Asp Ile Gly Gly His Glu Asn 
1               5                   10                  15 

gtc gac atc atc gcc gta gaa gtt aaa gcg ggc gac acc atc gcc gtt       96 
Val Asp Ile Ile Ala Val Glu Val Lys Ala Gly Asp Thr Ile Ala Val 
            20                  25                  30 

gac gac acc ctg att aca ctg gaa acc gac aaa gcc acg atg gat gtg      144 
Asp Asp Thr Leu Ile Thr Leu Glu Thr Asp Lys Ala Thr Met Asp Val 
        35                  40                  45 

cct gcc gat gcg gcc ggt gtc gtg aaa gaa gtg aaa gtc aaa gtc ggc      192 
Pro Ala Asp Ala Ala Gly Val Val Lys Glu Val Lys Val Lys Val Gly 
    50                  55                  60 

gac aaa atc tcc gaa ggc ggc gta att ctg acc gtt gaa acc ggt gcc      240 
Asp Lys Ile Ser Glu Gly Gly Val Ile Leu Thr Val Glu Thr Gly Ala 
65                  70                  75                  80 

gcc gcc gcc gaa gcc gcc ccg gct gct gcc gaa gca caa cct gca cct      288 
Ala Ala Ala Glu Ala Ala Pro Ala Ala Ala Glu Ala Gln Pro Ala Pro 
                85                  90                  95 

gct gcc gca ccc gct gcc gca ggc ggt gca acc gtt caa gta gcc gtt      336 
Ala Ala Ala Pro Ala Ala Ala Gly Gly Ala Thr Val Gln Val Ala Val 
            100                 105                 110 

ccc gat atc ggc ggc cat acc gat gtg gat gta atc gcc gtt gaa atc      384 
Pro Asp Ile Gly Gly His Thr Asp Val Asp Val Ile Ala Val Glu Ile 
        115                 120                 125 

aaa gtg ggc gac acc gtt gcc gaa gac gac acg ctg att act ttg gaa      432 
Lys Val Gly Asp Thr Val Ala Glu Asp Asp Thr Leu Ile Thr Leu Glu 
    130                 135                 140 

acc gat aaa gcg aca atg gac gta cct tgt acc gct gcc ggt gtc gtt      480 
Thr Asp Lys Ala Thr Met Asp Val Pro Cys Thr Ala Ala Gly Val Val 
145                 150                 155                 160 

aaa gcc gta ttc tta aaa gtc ggc gac aaa gta tcc gaa ggc tct gcc      528 
Lys Ala Val Phe Leu Lys Val Gly Asp Lys Val Ser Glu Gly Ser Ala 
                165                 170                 175 

att atc gaa gta gaa acc gtc ggc tct gcc gca gca gcc cct gct caa      576 
Ile Ile Glu Val Glu Thr Val Gly Ser Ala Ala Ala Ala Pro Ala Gln 
            180                 185                 190 

gcc gct caa gct gcc gca ccg gct gcc gct ccg cct ccg act gct gcc      624 
Ala Ala Gln Ala Ala Ala Pro Ala Ala Ala Pro Pro Pro Thr Ala Ala 
        195                 200                 205 

gcc gca ccc gcc gcc gcg cct gca cct tct gca cct gcc gct gcc aaa      672 
Ala Ala Pro Ala Ala Ala Pro Ala Pro Ser Ala Pro Ala Ala Ala Lys 
    210                 215                 220 

atc gac gag gcc gct ttc gcc aaa gca cac gcc ggc cct tcc gca cgc      720 
Ile Asp Glu Ala Ala Phe Ala Lys Ala His Ala Gly Pro Ser Ala Arg 
225                 230                 235                 240 

aaa ctg gcg cgc gaa ttg ggc gtg gat ttg ggc caa gtc aaa ggt acc      768 
Lys Leu Ala Arg Glu Leu Gly Val Asp Leu Gly Gln Val Lys Gly Thr 
                245                 250                 255 

ggc ttg aaa ggc cgc atc gtg ggc gac gac atc aaa gcc ttt gtg aaa      816 
Gly Leu Lys Gly Arg Ile Val Gly Asp Asp Ile Lys Ala Phe Val Lys 
            260                 265                 270 

tcc gta atg cag ggc ggc gcg gca aaa cct gcc gca gtc ggc gcg tct      864 
Ser Val Met Gln Gly Gly Ala Ala Lys Pro Ala Ala Val Gly Ala Ser 
        275                 280                 285 

tta ggc ggc ggt ctg gac ttg ctg ccc tgg cct aaa gtg gac ttc tcc      912 
Leu Gly Gly Gly Leu Asp Leu Leu Pro Trp Pro Lys Val Asp Phe Ser 
    290                 295                 300 

aaa ttc ggt aat gtc gaa gtt aaa gaa ttg tcc cgc att aag aaa att      960 
Lys Phe Gly Asn Val Glu Val Lys Glu Leu Ser Arg Ile Lys Lys Ile 
305                 310                 315                 320 

tcc ggt caa aac ctg tct cgc aac tgg gtg gtg att ccc cac gtt acc     1008 
Ser Gly Gln Asn Leu Ser Arg Asn Trp Val Val Ile Pro His Val Thr 
                325                 330                 335 

gta cac gaa gaa gca gat atg acc gag ctg gaa gaa ttc cgc aaa caa     1056 
Val His Glu Glu Ala Asp Met Thr Glu Leu Glu Glu Phe Arg Lys Gln 
            340                 345                 350 

ctg aac aaa gaa tgg gaa cgc gaa ggc gtg aaa ctg tca ccg ttg gcg     1104 
Leu Asn Lys Glu Trp Glu Arg Glu Gly Val Lys Leu Ser Pro Leu Ala 
        355                 360                 365 

ttc atc atc aaa gcc tcc gtt tcc gcg ctg aaa gcc ttc ccc gaa ttc     1152 
Phe Ile Ile Lys Ala Ser Val Ser Ala Leu Lys Ala Phe Pro Glu Phe 
    370                 375                 380 

aac gct tct ctg gac ggc gac aac ttg gtg ctg aaa aac tac ttc aac     1200 
Asn Ala Ser Leu Asp Gly Asp Asn Leu Val Leu Lys Asn Tyr Phe Asn 
385                 390                 395                 400 

atc ggt ttc gca gca gac acg ccg aac ggc ttg gtt gtt cca gta atc     1248 
Ile Gly Phe Ala Ala Asp Thr Pro Asn Gly Leu Val Val Pro Val Ile 
                405                 410                 415 

aaa gac gtg gat caa aaa ggc ttg aaa caa atc agc caa gag ctg acc     1296 
Lys Asp Val Asp Gln Lys Gly Leu Lys Gln Ile Ser Gln Glu Leu Thr 
            420                 425                 430 

gaa ttg tct aaa aaa gcc cgc gaa ggc aag ctc aaa cca caa gaa atg     1344 
Glu Leu Ser Lys Lys Ala Arg Glu Gly Lys Leu Lys Pro Gln Glu Met 
        435                 440                 445 

caa ggc gca tgc ttt acc att tcc agc ttg ggc ggc atc ggc ggc aca     1392 
Gln Gly Ala Cys Phe Thr Ile Ser Ser Leu Gly Gly Ile Gly Gly Thr 
    450                 455                 460 

ggt ttc acg ccg att gtg aac gct ccc gaa gtc gcc atc ttg ggc gtg     1440 
Gly Phe Thr Pro Ile Val Asn Ala Pro Glu Val Ala Ile Leu Gly Val 
465                 470                 475                 480 

tgc aaa tcc caa atc aaa ccg gtt tgg aac ggc aaa gaa ttc gct cct     1488 
Cys Lys Ser Gln Ile Lys Pro Val Trp Asn Gly Lys Glu Phe Ala Pro 
                485                 490                 495 

cgc ctg atg tgc cca ttg agc ctg tcc ttt gac cac cgt gtc atc gac     1536 
Arg Leu Met Cys Pro Leu Ser Leu Ser Phe Asp His Arg Val Ile Asp 
            500                 505                 510 

ggc gcg gcc ggt atg cgc ttc acc gta ttc ctg gcc aac ctg ttg aaa     1584 
Gly Ala Ala Gly Met Arg Phe Thr Val Phe Leu Ala Asn Leu Leu Lys 
        515                 520                 525 

gac ttc cgc cgc att act ctg                                         1605 
Asp Phe Arg Arg Ile Thr Leu 
    530                 535 

 
           
             18  
             535  
             PRT  
             Neisseria meningitidis  
           
            18 

Met Ser Ile Val Glu Ile Lys Val Pro Asp Ile Gly Gly His Glu Asn 
1               5                   10                  15 

Val Asp Ile Ile Ala Val Glu Val Lys Ala Gly Asp Thr Ile Ala Val 
            20                  25                  30 

Asp Asp Thr Leu Ile Thr Leu Glu Thr Asp Lys Ala Thr Met Asp Val 
        35                  40                  45 

Pro Ala Asp Ala Ala Gly Val Val Lys Glu Val Lys Val Lys Val Gly 
    50                  55                  60 

Asp Lys Ile Ser Glu Gly Gly Val Ile Leu Thr Val Glu Thr Gly Ala 
65                  70                  75                  80 

Ala Ala Ala Glu Ala Ala Pro Ala Ala Ala Glu Ala Gln Pro Ala Pro 
                85                  90                  95 

Ala Ala Ala Pro Ala Ala Ala Gly Gly Ala Thr Val Gln Val Ala Val 
            100                 105                 110 

Pro Asp Ile Gly Gly His Thr Asp Val Asp Val Ile Ala Val Glu Ile 
        115                 120                 125 

Lys Val Gly Asp Thr Val Ala Glu Asp Asp Thr Leu Ile Thr Leu Glu 
    130                 135                 140 

Thr Asp Lys Ala Thr Met Asp Val Pro Cys Thr Ala Ala Gly Val Val 
145                 150                 155                 160 

Lys Ala Val Phe Leu Lys Val Gly Asp Lys Val Ser Glu Gly Ser Ala 
                165                 170                 175 

Ile Ile Glu Val Glu Thr Val Gly Ser Ala Ala Ala Ala Pro Ala Gln 
            180                 185                 190 

Ala Ala Gln Ala Ala Ala Pro Ala Ala Ala Pro Pro Pro Thr Ala Ala 
        195                 200                 205 

Ala Ala Pro Ala Ala Ala Pro Ala Pro Ser Ala Pro Ala Ala Ala Lys 
    210                 215                 220 

Ile Asp Glu Ala Ala Phe Ala Lys Ala His Ala Gly Pro Ser Ala Arg 
225                 230                 235                 240 

Lys Leu Ala Arg Glu Leu Gly Val Asp Leu Gly Gln Val Lys Gly Thr 
                245                 250                 255 

Gly Leu Lys Gly Arg Ile Val Gly Asp Asp Ile Lys Ala Phe Val Lys 
            260                 265                 270 

Ser Val Met Gln Gly Gly Ala Ala Lys Pro Ala Ala Val Gly Ala Ser 
        275                 280                 285 

Leu Gly Gly Gly Leu Asp Leu Leu Pro Trp Pro Lys Val Asp Phe Ser 
    290                 295                 300 

Lys Phe Gly Asn Val Glu Val Lys Glu Leu Ser Arg Ile Lys Lys Ile 
305                 310                 315                 320 

Ser Gly Gln Asn Leu Ser Arg Asn Trp Val Val Ile Pro His Val Thr 
                325                 330                 335 

Val His Glu Glu Ala Asp Met Thr Glu Leu Glu Glu Phe Arg Lys Gln 
            340                 345                 350 

Leu Asn Lys Glu Trp Glu Arg Glu Gly Val Lys Leu Ser Pro Leu Ala 
        355                 360                 365 

Phe Ile Ile Lys Ala Ser Val Ser Ala Leu Lys Ala Phe Pro Glu Phe 
    370                 375                 380 

Asn Ala Ser Leu Asp Gly Asp Asn Leu Val Leu Lys Asn Tyr Phe Asn 
385                 390                 395                 400 

Ile Gly Phe Ala Ala Asp Thr Pro Asn Gly Leu Val Val Pro Val Ile 
                405                 410                 415 

Lys Asp Val Asp Gln Lys Gly Leu Lys Gln Ile Ser Gln Glu Leu Thr 
            420                 425                 430 

Glu Leu Ser Lys Lys Ala Arg Glu Gly Lys Leu Lys Pro Gln Glu Met 
        435                 440                 445 

Gln Gly Ala Cys Phe Thr Ile Ser Ser Leu Gly Gly Ile Gly Gly Thr 
    450                 455                 460 

Gly Phe Thr Pro Ile Val Asn Ala Pro Glu Val Ala Ile Leu Gly Val 
465                 470                 475                 480 

Cys Lys Ser Gln Ile Lys Pro Val Trp Asn Gly Lys Glu Phe Ala Pro 
                485                 490                 495 

Arg Leu Met Cys Pro Leu Ser Leu Ser Phe Asp His Arg Val Ile Asp 
            500                 505                 510 

Gly Ala Ala Gly Met Arg Phe Thr Val Phe Leu Ala Asn Leu Leu Lys 
        515                 520                 525 

Asp Phe Arg Arg Ile Thr Leu 
    530                 535 

 
           
             19  
             321  
             DNA  
             Neisseria meningitidis  
             
               CDS  
               (1)..(321)  
               NMA0039  
             
           
            19 

atg ttt aag gac gaa cta aat gaa ttt atc cga ttg ata tcc gat ccg       48 
Met Phe Lys Asp Glu Leu Asn Glu Phe Ile Arg Leu Ile Ser Asp Pro 
1               5                   10                  15 

gaa agt gaa ttg gat gaa tgg tat ttg agt gat ttt aaa gat gaa cat       96 
Glu Ser Glu Leu Asp Glu Trp Tyr Leu Ser Asp Phe Lys Asp Glu His 
            20                  25                  30 

ata tgg gaa atg caa agt tac gaa gca ttt tca tgt ttg cgg gaa gca      144 
Ile Trp Glu Met Gln Ser Tyr Glu Ala Phe Ser Cys Leu Arg Glu Ala 
        35                  40                  45 

gtc ccc tat ttg ttt gct tat ccc cga tac ggg tat gaa ctg ttg gag      192 
Val Pro Tyr Leu Phe Ala Tyr Pro Arg Tyr Gly Tyr Glu Leu Leu Glu 
    50                  55                  60 

ata att tcc gcg tta aaa gaa aca tct gat acg aca gaa tta ttt tat      240 
Ile Ile Ser Ala Leu Lys Glu Thr Ser Asp Thr Thr Glu Leu Phe Tyr 
65                  70                  75                  80 

gag ccg ggt atc gtg cct ttg ctg att gct tta tat aaa gaa gat agt      288 
Glu Pro Gly Ile Val Pro Leu Leu Ile Ala Leu Tyr Lys Glu Asp Ser 
                85                  90                  95 

tat ctg gta aat atg gtc aaa cgg ata ttc aaa                          321 
Tyr Leu Val Asn Met Val Lys Arg Ile Phe Lys 
            100                 105 

 
           
             20  
             107  
             PRT  
             Neisseria meningitidis  
           
            20 

Met Phe Lys Asp Glu Leu Asn Glu Phe Ile Arg Leu Ile Ser Asp Pro 
1               5                   10                  15 

Glu Ser Glu Leu Asp Glu Trp Tyr Leu Ser Asp Phe Lys Asp Glu His 
            20                  25                  30 

Ile Trp Glu Met Gln Ser Tyr Glu Ala Phe Ser Cys Leu Arg Glu Ala 
        35                  40                  45 

Val Pro Tyr Leu Phe Ala Tyr Pro Arg Tyr Gly Tyr Glu Leu Leu Glu 
    50                  55                  60 

Ile Ile Ser Ala Leu Lys Glu Thr Ser Asp Thr Thr Glu Leu Phe Tyr 
65                  70                  75                  80 

Glu Pro Gly Ile Val Pro Leu Leu Ile Ala Leu Tyr Lys Glu Asp Ser 
                85                  90                  95 

Tyr Leu Val Asn Met Val Lys Arg Ile Phe Lys 
            100                 105 

 
           
             21  
             279  
             DNA  
             Neisseria meningitidis  
             
               CDS  
               (1)..(279)  
               NMA0038  
             
           
            21 

atg gaa aac ggg caa cgg aat aac cgg ttt cct ttg gaa aaa cga att       48 
Met Glu Asn Gly Gln Arg Asn Asn Arg Phe Pro Leu Glu Lys Arg Ile 
1               5                   10                  15 

ttt tat ctg gag cat tcg ggg cgg tat ctg atg att tgc gcc tta tcg       96 
Phe Tyr Leu Glu His Ser Gly Arg Tyr Leu Met Ile Cys Ala Leu Ser 
            20                  25                  30 

gat tat tcc caa aac aaa cat act gtc gtt atg gca aat ttc atc tat      144 
Asp Tyr Ser Gln Asn Lys His Thr Val Val Met Ala Asn Phe Ile Tyr 
        35                  40                  45 

ccg gat gaa aaa acg gat tgg cgg aat ttg gat gat tta ttc aat gag      192 
Pro Asp Glu Lys Thr Asp Trp Arg Asn Leu Asp Asp Leu Phe Asn Glu 
    50                  55                  60 

ttg gtt tta gag gaa ttg cag gct tca ttt atg gat tgg cat ccg act      240 
Leu Val Leu Glu Glu Leu Gln Ala Ser Phe Met Asp Trp His Pro Thr 
65                  70                  75                  80 

gtt gaa gag gca atc agc cgt cat ttg gaa gac ttt tcg                  279 
Val Glu Glu Ala Ile Ser Arg His Leu Glu Asp Phe Ser 
                85                  90 

 
           
             22  
             93  
             PRT  
             Neisseria meningitidis  
           
            22 

Met Glu Asn Gly Gln Arg Asn Asn Arg Phe Pro Leu Glu Lys Arg Ile 
1               5                   10                  15 

Phe Tyr Leu Glu His Ser Gly Arg Tyr Leu Met Ile Cys Ala Leu Ser 
            20                  25                  30 

Asp Tyr Ser Gln Asn Lys His Thr Val Val Met Ala Asn Phe Ile Tyr 
        35                  40                  45 

Pro Asp Glu Lys Thr Asp Trp Arg Asn Leu Asp Asp Leu Phe Asn Glu 
    50                  55                  60 

Leu Val Leu Glu Glu Leu Gln Ala Ser Phe Met Asp Trp His Pro Thr 
65                  70                  75                  80 

Val Glu Glu Ala Ile Ser Arg His Leu Glu Asp Phe Ser 
                85                  90 

 
           
             23  
             471  
             DNA  
             Neisseria meningitidis  
             
               CDS  
               (1)..(471)  
               NMB0222  
             
           
            23 

atg aac ctt gat tta acc gcg caa aaa gtc cgt ctt tct tgg aag gat       48 
Met Asn Leu Asp Leu Thr Ala Gln Lys Val Arg Leu Ser Trp Lys Asp 
1               5                   10                  15 

att ctg tgg ggg tat ggg aat aaa tac ttg ggt tgg gct gat gtg gca       96 
Ile Leu Trp Gly Tyr Gly Asn Lys Tyr Leu Gly Trp Ala Asp Val Ala 
            20                  25                  30 

gct tat gcc cga aaa atg acg ctt tca gat cat gat gaa cgt gtg ttc      144 
Ala Tyr Ala Arg Lys Met Thr Leu Ser Asp His Asp Glu Arg Val Phe 
        35                  40                  45 

aaa cta tct tta atc aac aaa tcc aat att ctt gaa tta aag cct gtt      192 
Lys Leu Ser Leu Ile Asn Lys Ser Asn Ile Leu Glu Leu Lys Pro Val 
    50                  55                  60 

ctg gaa gat ttg gct tcg gaa atg agg gat tat tcc cct aaa aat tgg      240 
Leu Glu Asp Leu Ala Ser Glu Met Arg Asp Tyr Ser Pro Lys Asn Trp 
65                  70                  75                  80 

ctg tac gtc ctc tta agc gat gta ttc cat aga aaa gaa gaa ttt gag      288 
Leu Tyr Val Leu Leu Ser Asp Val Phe His Arg Lys Glu Glu Phe Glu 
                85                  90                  95 

gat cct ttg ggg gaa gtt gaa aaa att tat gca gat ttt gat tat ccg      336 
Asp Pro Leu Gly Glu Val Glu Lys Ile Tyr Ala Asp Phe Asp Tyr Pro 
            100                 105                 110 

gaa gaa ata gaa tca ttt gtc agg tat atg ccg ccc aaa gac ggt tat      384 
Glu Glu Ile Glu Ser Phe Val Arg Tyr Met Pro Pro Lys Asp Gly Tyr 
        115                 120                 125 

att cct tct gcc cac acc tat gaa gaa aat att gcc cgg tta tat tct      432 
Ile Pro Ser Ala His Thr Tyr Glu Glu Asn Ile Ala Arg Leu Tyr Ser 
    130                 135                 140 

cac tgg gaa cac tat ttg aac aac ggc gga ggg cag ggt                  471 
His Trp Glu His Tyr Leu Asn Asn Gly Gly Gly Gln Gly 
145                 150                 155 

 
           
             24  
             157  
             PRT  
             Neisseria meningitidis  
           
            24 

Met Asn Leu Asp Leu Thr Ala Gln Lys Val Arg Leu Ser Trp Lys Asp 
1               5                   10                  15 

Ile Leu Trp Gly Tyr Gly Asn Lys Tyr Leu Gly Trp Ala Asp Val Ala 
            20                  25                  30 

Ala Tyr Ala Arg Lys Met Thr Leu Ser Asp His Asp Glu Arg Val Phe 
        35                  40                  45 

Lys Leu Ser Leu Ile Asn Lys Ser Asn Ile Leu Glu Leu Lys Pro Val 
    50                  55                  60 

Leu Glu Asp Leu Ala Ser Glu Met Arg Asp Tyr Ser Pro Lys Asn Trp 
65                  70                  75                  80 

Leu Tyr Val Leu Leu Ser Asp Val Phe His Arg Lys Glu Glu Phe Glu 
                85                  90                  95 

Asp Pro Leu Gly Glu Val Glu Lys Ile Tyr Ala Asp Phe Asp Tyr Pro 
            100                 105                 110 

Glu Glu Ile Glu Ser Phe Val Arg Tyr Met Pro Pro Lys Asp Gly Tyr 
        115                 120                 125 

Ile Pro Ser Ala His Thr Tyr Glu Glu Asn Ile Ala Arg Leu Tyr Ser 
    130                 135                 140 

His Trp Glu His Tyr Leu Asn Asn Gly Gly Gly Gln Gly 
145                 150                 155 

 
           
             25  
             333  
             DNA  
             Neisseria meningitidis  
             
               CDS  
               (1)..(333)  
               NMA0036  
             
           
            25 

atg acg gtt gat aag gaa caa gta gaa aag atg att tat tct gaa aat       48 
Met Thr Val Asp Lys Glu Gln Val Glu Lys Met Ile Tyr Ser Glu Asn 
1               5                   10                  15 

aaa cag agt gtt att gac ggt atg ctc ggt atg acc ttc agc tcg gag       96 
Lys Gln Ser Val Ile Asp Gly Met Leu Gly Met Thr Phe Ser Ser Glu 
            20                  25                  30 

gag gat gaa atc ccc tgg att tcc gag aag ttg acg gag ttg tcg aaa      144 
Glu Asp Glu Ile Pro Trp Ile Ser Glu Lys Leu Thr Glu Leu Ser Lys 
        35                  40                  45 

cat aaa gac ttg gat att gcc aga ttg tca tta acc tgt ttc gga cat      192 
His Lys Asp Leu Asp Ile Ala Arg Leu Ser Leu Thr Cys Phe Gly His 
    50                  55                  60 

ctc gcc agg atg cat gaa aat atc ggc gat tgc gat aaa gtg att gct      240 
Leu Ala Arg Met His Glu Asn Ile Gly Asp Cys Asp Lys Val Ile Ala 
65                  70                  75                  80 

tta ttg ctc tca aaa caa ggt gat ccg gat ttt caa ggt ttt gcc gaa      288 
Leu Leu Leu Ser Lys Gln Gly Asp Pro Asp Phe Gln Gly Phe Ala Glu 
                85                  90                  95 

gat gcg tta gac gag att tct tta ttt ata ttt aaa aag cgt ccg          333 
Asp Ala Leu Asp Glu Ile Ser Leu Phe Ile Phe Lys Lys Arg Pro 
            100                 105                 110 

 
           
             26  
             111  
             PRT  
             Neisseria meningitidis  
           
            26 

Met Thr Val Asp Lys Glu Gln Val Glu Lys Met Ile Tyr Ser Glu Asn 
1               5                   10                  15 

Lys Gln Ser Val Ile Asp Gly Met Leu Gly Met Thr Phe Ser Ser Glu 
            20                  25                  30 

Glu Asp Glu Ile Pro Trp Ile Ser Glu Lys Leu Thr Glu Leu Ser Lys 
        35                  40                  45 

His Lys Asp Leu Asp Ile Ala Arg Leu Ser Leu Thr Cys Phe Gly His 
    50                  55                  60 

Leu Ala Arg Met His Glu Asn Ile Gly Asp Cys Asp Lys Val Ile Ala 
65                  70                  75                  80 

Leu Leu Leu Ser Lys Gln Gly Asp Pro Asp Phe Gln Gly Phe Ala Glu 
                85                  90                  95 

Asp Ala Leu Asp Glu Ile Ser Leu Phe Ile Phe Lys Lys Arg Pro 
            100                 105                 110 

 
           
             27  
             1338  
             DNA  
             Neisseria meningitidis  
             
               CDS  
               (1)..(1338)  
               NMB1645  
             
           
            27 

atg ttg aat cca tcc cga aaa ctg gtt gag ctg gtc cgt att ttg gac       48 
Met Leu Asn Pro Ser Arg Lys Leu Val Glu Leu Val Arg Ile Leu Asp 
1               5                   10                  15 

gaa ggc ggt ttt att ttc agc ggc gat ccc gta cag gcg acg gag gct       96 
Glu Gly Gly Phe Ile Phe Ser Gly Asp Pro Val Gln Ala Thr Glu Ala 
            20                  25                  30 

ttg cgc cgc gtg gac ggc agt acg gag gaa aaa atc atc cgt cgg gcg      144 
Leu Arg Arg Val Asp Gly Ser Thr Glu Glu Lys Ile Ile Arg Arg Ala 
        35                  40                  45 

gag atg att gac agg aac cgt atg ctg cgg gag acg ttg gaa cgt gtg      192 
Glu Met Ile Asp Arg Asn Arg Met Leu Arg Glu Thr Leu Glu Arg Val 
    50                  55                  60 

cgt gcg ggg tcg ttc tgg ttg tgg gtg gtg gcg gcg acg ttt gca ttt      240 
Arg Ala Gly Ser Phe Trp Leu Trp Val Val Ala Ala Thr Phe Ala Phe 
65                  70                  75                  80 

ttt acc ggt ttt tca gtc act tat ctt cta atg gac aat cag ggt ctg      288 
Phe Thr Gly Phe Ser Val Thr Tyr Leu Leu Met Asp Asn Gln Gly Leu 
                85                  90                  95 

aat ttc ttt ttg gtt ttg gcg ggc gtg ttg ggc atg aat acg ctg atg      336 
Asn Phe Phe Leu Val Leu Ala Gly Val Leu Gly Met Asn Thr Leu Met 
            100                 105                 110 

ctg gca gta tgg ttg gca atg ttg ttc ctg cgt gtg aaa gtg ggg cgt      384 
Leu Ala Val Trp Leu Ala Met Leu Phe Leu Arg Val Lys Val Gly Arg 
        115                 120                 125 

ttt ttc agc agt ccg gcg acg tgg ttt cgg ggc aaa gac cct gta aat      432 
Phe Phe Ser Ser Pro Ala Thr Trp Phe Arg Gly Lys Asp Pro Val Asn 
    130                 135                 140 

cag gcg gtg ttg cgg ctg tat gcg gac gag tgg cgg caa cct tcg gta      480 
Gln Ala Val Leu Arg Leu Tyr Ala Asp Glu Trp Arg Gln Pro Ser Val 
145                 150                 155                 160 

cgt tgg aaa ata ggc gca acg tcg cac agc ctg tgg ctc tgc acg ctg      528 
Arg Trp Lys Ile Gly Ala Thr Ser His Ser Leu Trp Leu Cys Thr Leu 
                165                 170                 175 

ctc gga atg ctg gtg tcg gta ttg ttg ctg ctt ttg gtg cgg caa tat      576 
Leu Gly Met Leu Val Ser Val Leu Leu Leu Leu Leu Val Arg Gln Tyr 
            180                 185                 190 

acg ttc aac tgg gaa agc acg ctg ttg agc aat gcc gct tcg gta cgc      624 
Thr Phe Asn Trp Glu Ser Thr Leu Leu Ser Asn Ala Ala Ser Val Arg 
        195                 200                 205 

gcg gtg gaa atg ttg gca tgg ctg ccg tcg aaa ctc ggt ttc cct gtc      672 
Ala Val Glu Met Leu Ala Trp Leu Pro Ser Lys Leu Gly Phe Pro Val 
    210                 215                 220 

ccc gat gcg cgg gcg gtc atc gaa ggc cgt ctg aac ggc aat att gcc      720 
Pro Asp Ala Arg Ala Val Ile Glu Gly Arg Leu Asn Gly Asn Ile Ala 
225                 230                 235                 240 

gat gcg cgg gct tgg tcg ggg ctg ctg gtc ggc agt atc gcc tgc tac      768 
Asp Ala Arg Ala Trp Ser Gly Leu Leu Val Gly Ser Ile Ala Cys Tyr 
                245                 250                 255 

ggc atc ctg ccg cgc ctg ctg gct tgg gta gtg tgt aaa atc ctt ttg      816 
Gly Ile Leu Pro Arg Leu Leu Ala Trp Val Val Cys Lys Ile Leu Leu 
            260                 265                 270 

aaa aca agc gaa aac gga ttg gat ttg gaa aag ccc tat tat cag gcg      864 
Lys Thr Ser Glu Asn Gly Leu Asp Leu Glu Lys Pro Tyr Tyr Gln Ala 
        275                 280                 285 

gtc atc cgc cgc tgg cag aac aaa atc acc gat gcg gat acg cgt cgg      912 
Val Ile Arg Arg Trp Gln Asn Lys Ile Thr Asp Ala Asp Thr Arg Arg 
    290                 295                 300 

gaa acc gtg tcc gcc gtt tca ccg aaa atc atc ttg aac gat gcg ccg      960 
Glu Thr Val Ser Ala Val Ser Pro Lys Ile Ile Leu Asn Asp Ala Pro 
305                 310                 315                 320 

aaa tgg gcg gtc atg ctg gag acc gag tgg cag gac ggc gaa tgg ttc     1008 
Lys Trp Ala Val Met Leu Glu Thr Glu Trp Gln Asp Gly Glu Trp Phe 
                325                 330                 335 

gag ggc agg ctg gcg cag gaa tgg ctg gat aag ggc gtt gcc acc aat     1056 
Glu Gly Arg Leu Ala Gln Glu Trp Leu Asp Lys Gly Val Ala Thr Asn 
            340                 345                 350 

cgg gaa cag gtt gcc gcg ctg gag aca gag ctg aag cag aaa ccg gcg     1104 
Arg Glu Gln Val Ala Ala Leu Glu Thr Glu Leu Lys Gln Lys Pro Ala 
        355                 360                 365 

caa ctg ctt atc ggc gtg cgc gcc caa act gtg ccg gac cgc ggc gtg     1152 
Gln Leu Leu Ile Gly Val Arg Ala Gln Thr Val Pro Asp Arg Gly Val 
    370                 375                 380 

ttg cgg cag att gtc cga ctc tcg gaa gcg gcg cag ggc ggc gcg gtg     1200 
Leu Arg Gln Ile Val Arg Leu Ser Glu Ala Ala Gln Gly Gly Ala Val 
385                 390                 395                 400 

gtg cag ctt ttg gcg gaa cag ggg ctt tca gac gac ctt tcg gaa aag     1248 
Val Gln Leu Leu Ala Glu Gln Gly Leu Ser Asp Asp Leu Ser Glu Lys 
                405                 410                 415 

ctg gaa cat tgg cgt aac gcg ctg gcc gaa tgc ggc gcg gcg tgg ctt     1296 
Leu Glu His Trp Arg Asn Ala Leu Ala Glu Cys Gly Ala Ala Trp Leu 
            420                 425                 430 

gag cct gac agg gcg gcg cag gaa ggg cgt ttg aaa gac caa             1338 
Glu Pro Asp Arg Ala Ala Gln Glu Gly Arg Leu Lys Asp Gln 
        435                 440                 445 

 
           
             28  
             446  
             PRT  
             Neisseria meningitidis  
           
            28 

Met Leu Asn Pro Ser Arg Lys Leu Val Glu Leu Val Arg Ile Leu Asp 
1               5                   10                  15 

Glu Gly Gly Phe Ile Phe Ser Gly Asp Pro Val Gln Ala Thr Glu Ala 
            20                  25                  30 

Leu Arg Arg Val Asp Gly Ser Thr Glu Glu Lys Ile Ile Arg Arg Ala 
        35                  40                  45 

Glu Met Ile Asp Arg Asn Arg Met Leu Arg Glu Thr Leu Glu Arg Val 
    50                  55                  60 

Arg Ala Gly Ser Phe Trp Leu Trp Val Val Ala Ala Thr Phe Ala Phe 
65                  70                  75                  80 

Phe Thr Gly Phe Ser Val Thr Tyr Leu Leu Met Asp Asn Gln Gly Leu 
                85                  90                  95 

Asn Phe Phe Leu Val Leu Ala Gly Val Leu Gly Met Asn Thr Leu Met 
            100                 105                 110 

Leu Ala Val Trp Leu Ala Met Leu Phe Leu Arg Val Lys Val Gly Arg 
        115                 120                 125 

Phe Phe Ser Ser Pro Ala Thr Trp Phe Arg Gly Lys Asp Pro Val Asn 
    130                 135                 140 

Gln Ala Val Leu Arg Leu Tyr Ala Asp Glu Trp Arg Gln Pro Ser Val 
145                 150                 155                 160 

Arg Trp Lys Ile Gly Ala Thr Ser His Ser Leu Trp Leu Cys Thr Leu 
                165                 170                 175 

Leu Gly Met Leu Val Ser Val Leu Leu Leu Leu Leu Val Arg Gln Tyr 
            180                 185                 190 

Thr Phe Asn Trp Glu Ser Thr Leu Leu Ser Asn Ala Ala Ser Val Arg 
        195                 200                 205 

Ala Val Glu Met Leu Ala Trp Leu Pro Ser Lys Leu Gly Phe Pro Val 
    210                 215                 220 

Pro Asp Ala Arg Ala Val Ile Glu Gly Arg Leu Asn Gly Asn Ile Ala 
225                 230                 235                 240 

Asp Ala Arg Ala Trp Ser Gly Leu Leu Val Gly Ser Ile Ala Cys Tyr 
                245                 250                 255 

Gly Ile Leu Pro Arg Leu Leu Ala Trp Val Val Cys Lys Ile Leu Leu 
            260                 265                 270 

Lys Thr Ser Glu Asn Gly Leu Asp Leu Glu Lys Pro Tyr Tyr Gln Ala 
        275                 280                 285 

Val Ile Arg Arg Trp Gln Asn Lys Ile Thr Asp Ala Asp Thr Arg Arg 
    290                 295                 300 

Glu Thr Val Ser Ala Val Ser Pro Lys Ile Ile Leu Asn Asp Ala Pro 
305                 310                 315                 320 

Lys Trp Ala Val Met Leu Glu Thr Glu Trp Gln Asp Gly Glu Trp Phe 
                325                 330                 335 

Glu Gly Arg Leu Ala Gln Glu Trp Leu Asp Lys Gly Val Ala Thr Asn 
            340                 345                 350 

Arg Glu Gln Val Ala Ala Leu Glu Thr Glu Leu Lys Gln Lys Pro Ala 
        355                 360                 365 

Gln Leu Leu Ile Gly Val Arg Ala Gln Thr Val Pro Asp Arg Gly Val 
    370                 375                 380 

Leu Arg Gln Ile Val Arg Leu Ser Glu Ala Ala Gln Gly Gly Ala Val 
385                 390                 395                 400 

Val Gln Leu Leu Ala Glu Gln Gly Leu Ser Asp Asp Leu Ser Glu Lys 
                405                 410                 415 

Leu Glu His Trp Arg Asn Ala Leu Ala Glu Cys Gly Ala Ala Trp Leu 
            420                 425                 430 

Glu Pro Asp Arg Ala Ala Gln Glu Gly Arg Leu Lys Asp Gln 
        435                 440                 445 

 
           
             29  
             1392  
             DNA  
             Neisseria meningitidis  
             
               CDS  
               (1)..(1392)  
               NMA1898  
             
           
            29 

atg aac aaa caa ccc ctt tcc ctc gcc gtc gtc ggg cat acc aat acc       48 
Met Asn Lys Gln Pro Leu Ser Leu Ala Val Val Gly His Thr Asn Thr 
1               5                   10                  15 

ggc aaa acc tcg ctc ctg cgc acc cta ttg cgc gac agc ggc ttc ggc       96 
Gly Lys Thr Ser Leu Leu Arg Thr Leu Leu Arg Asp Ser Gly Phe Gly 
            20                  25                  30 

gaa gtc aaa aat gcc ccg tcc act acg cgc cat gtc gaa gaa gcc gcc      144 
Glu Val Lys Asn Ala Pro Ser Thr Thr Arg His Val Glu Glu Ala Ala 
        35                  40                  45 

atc agc gac ggc gca gac acg ctg gtt ttc ctg tat gac acg ccc gga      192 
Ile Ser Asp Gly Ala Asp Thr Leu Val Phe Leu Tyr Asp Thr Pro Gly 
    50                  55                  60 

ctc gaa gac gcg ggc ggt gtt ttg gaa tgg ctg gaa acc cat acg gat      240 
Leu Glu Asp Ala Gly Gly Val Leu Glu Trp Leu Glu Thr His Thr Asp 
65                  70                  75                  80 

acg cgt tca gac ggc atc gaa cgg ctg caa cag ttt ctc ggc agc cac      288 
Thr Arg Ser Asp Gly Ile Glu Arg Leu Gln Gln Phe Leu Gly Ser His 
                85                  90                  95 

ggc gcg cac cat gat ttc aat cag gaa gcc aaa gtc tta cgg caa gtc      336 
Gly Ala His His Asp Phe Asn Gln Glu Ala Lys Val Leu Arg Gln Val 
            100                 105                 110 

ttg caa agc gat atg gca atg tac gtc atc gac gcg cgc gaa ccc gtc      384 
Leu Gln Ser Asp Met Ala Met Tyr Val Ile Asp Ala Arg Glu Pro Val 
        115                 120                 125 

ctc gac aaa tac agg gac gag ttg acc atc ctt tca tgg tgt gcc aaa      432 
Leu Asp Lys Tyr Arg Asp Glu Leu Thr Ile Leu Ser Trp Cys Ala Lys 
    130                 135                 140 

ccg gtt atg ccc gtg ttc aac ttt acc ggc gga cag cct ccc gaa tcg      480 
Pro Val Met Pro Val Phe Asn Phe Thr Gly Gly Gln Pro Pro Glu Ser 
145                 150                 155                 160 

tgg aca acc atg ctg gcg agg aga aac ctg cac gtt ttc gca ggg ttc      528 
Trp Thr Thr Met Leu Ala Arg Arg Asn Leu His Val Phe Ala Gly Phe 
                165                 170                 175 

gac acc gtc gcc ttt gat ttt gaa ggc gaa cta cgc ctg tgg gaa aac      576 
Asp Thr Val Ala Phe Asp Phe Glu Gly Glu Leu Arg Leu Trp Glu Asn 
            180                 185                 190 

ctc gcc acc atg ttg ccc gaa cgc agc aca ctt gac cgc ctg aca gcc      624 
Leu Ala Thr Met Leu Pro Glu Arg Ser Thr Leu Asp Arg Leu Thr Ala 
        195                 200                 205 

atg cgc cgg cgc gaa tgg cag cgg ctg gac ggc gaa gcg cgc cgc gaa      672 
Met Arg Arg Arg Glu Trp Gln Arg Leu Asp Gly Glu Ala Arg Arg Glu 
    210                 215                 220 

atc gcc gac ttt tta att gat gcc gcc gcc ttc agg cag gaa gtg gac      720 
Ile Ala Asp Phe Leu Ile Asp Ala Ala Ala Phe Arg Gln Glu Val Asp 
225                 230                 235                 240 

gaa aac gag gat acc gcc acc gtg ccg caa acc atg cag gcg gaa ata      768 
Glu Asn Glu Asp Thr Ala Thr Val Pro Gln Thr Met Gln Ala Glu Ile 
                245                 250                 255 

cgc caa ctc gaa cgg cag atg cag cag cgg ctg ttt gcc ctt tac cgt      816 
Arg Gln Leu Glu Arg Gln Met Gln Gln Arg Leu Phe Ala Leu Tyr Arg 
            260                 265                 270 

ttc tac cac agc gaa atc gac ggc ggc gac tgg atg ccg caa gcc ttc      864 
Phe Tyr His Ser Glu Ile Asp Gly Gly Asp Trp Met Pro Gln Ala Phe 
        275                 280                 285 

cgc caa gac ccg ttc gac agc gaa ttg ctc aaa caa tac ggc atc cgc      912 
Arg Gln Asp Pro Phe Asp Ser Glu Leu Leu Lys Gln Tyr Gly Ile Arg 
    290                 295                 300 

acc ggc acg ggc gcg gca acc ggc gcg ctc atc ggc ttg ggg ctg gac      960 
Thr Gly Thr Gly Ala Ala Thr Gly Ala Leu Ile Gly Leu Gly Leu Asp 
305                 310                 315                 320 

atc gcc aca ctc ggc ggc tcg ctc gga ttg ggt acg gca atc ggc ggc     1008 
Ile Ala Thr Leu Gly Gly Ser Leu Gly Leu Gly Thr Ala Ile Gly Gly 
                325                 330                 335 

ttt ttg ggc ggc atc ctg ccc aat acc cgc acc att tct gac aaa ctc     1056 
Phe Leu Gly Gly Ile Leu Pro Asn Thr Arg Thr Ile Ser Asp Lys Leu 
            340                 345                 350 

gcc ggc cgt caa acc ctg cac acc gac ccc gaa acc ctg acc ctg ctc     1104 
Ala Gly Arg Gln Thr Leu His Thr Asp Pro Glu Thr Leu Thr Leu Leu 
        355                 360                 365 

gcc gcc cgc gcc ctc gat ctg ctc cac gtc ctg caa acg cgc gga cac     1152 
Ala Ala Arg Ala Leu Asp Leu Leu His Val Leu Gln Thr Arg Gly His 
    370                 375                 380 

gcg gca cag tcg gat atc gag ctg cac agc cgc aaa gtc ccg tgg gat     1200 
Ala Ala Gln Ser Asp Ile Glu Leu His Ser Arg Lys Val Pro Trp Asp 
385                 390                 395                 400 

gcc gcc aga ctc ccg ccc gaa ctc aac aaa gcc cgc agt cat tgg aaa     1248 
Ala Ala Arg Leu Pro Pro Glu Leu Asn Lys Ala Arg Ser His Trp Lys 
                405                 410                 415 

tgg tcg tcg ctc aat acg cac cgc ccg gaa acc agc cgc gcc gaa cgc     1296 
Trp Ser Ser Leu Asn Thr His Arg Pro Glu Thr Ser Arg Ala Glu Arg 
            420                 425                 430 

gcc gga tat gtg gag aaa ctt caa atc cgc ctg tcg gga aaa tat agt     1344 
Ala Gly Tyr Val Glu Lys Leu Gln Ile Arg Leu Ser Gly Lys Tyr Ser 
        435                 440                 445 

gaa tta aat tta aat cag gac aag gcg gcg agc cgc aga cag tac aaa     1392 
Glu Leu Asn Leu Asn Gln Asp Lys Ala Ala Ser Arg Arg Gln Tyr Lys 
    450                 455                 460 

 
           
             30  
             464  
             PRT  
             Neisseria meningitidis  
           
            30 

Met Asn Lys Gln Pro Leu Ser Leu Ala Val Val Gly His Thr Asn Thr 
1               5                   10                  15 

Gly Lys Thr Ser Leu Leu Arg Thr Leu Leu Arg Asp Ser Gly Phe Gly 
            20                  25                  30 

Glu Val Lys Asn Ala Pro Ser Thr Thr Arg His Val Glu Glu Ala Ala 
        35                  40                  45 

Ile Ser Asp Gly Ala Asp Thr Leu Val Phe Leu Tyr Asp Thr Pro Gly 
    50                  55                  60 

Leu Glu Asp Ala Gly Gly Val Leu Glu Trp Leu Glu Thr His Thr Asp 
65                  70                  75                  80 

Thr Arg Ser Asp Gly Ile Glu Arg Leu Gln Gln Phe Leu Gly Ser His 
                85                  90                  95 

Gly Ala His His Asp Phe Asn Gln Glu Ala Lys Val Leu Arg Gln Val 
            100                 105                 110 

Leu Gln Ser Asp Met Ala Met Tyr Val Ile Asp Ala Arg Glu Pro Val 
        115                 120                 125 

Leu Asp Lys Tyr Arg Asp Glu Leu Thr Ile Leu Ser Trp Cys Ala Lys 
    130                 135                 140 

Pro Val Met Pro Val Phe Asn Phe Thr Gly Gly Gln Pro Pro Glu Ser 
145                 150                 155                 160 

Trp Thr Thr Met Leu Ala Arg Arg Asn Leu His Val Phe Ala Gly Phe 
                165                 170                 175 

Asp Thr Val Ala Phe Asp Phe Glu Gly Glu Leu Arg Leu Trp Glu Asn 
            180                 185                 190 

Leu Ala Thr Met Leu Pro Glu Arg Ser Thr Leu Asp Arg Leu Thr Ala 
        195                 200                 205 

Met Arg Arg Arg Glu Trp Gln Arg Leu Asp Gly Glu Ala Arg Arg Glu 
    210                 215                 220 

Ile Ala Asp Phe Leu Ile Asp Ala Ala Ala Phe Arg Gln Glu Val Asp 
225                 230                 235                 240 

Glu Asn Glu Asp Thr Ala Thr Val Pro Gln Thr Met Gln Ala Glu Ile 
                245                 250                 255 

Arg Gln Leu Glu Arg Gln Met Gln Gln Arg Leu Phe Ala Leu Tyr Arg 
            260                 265                 270 

Phe Tyr His Ser Glu Ile Asp Gly Gly Asp Trp Met Pro Gln Ala Phe 
        275                 280                 285 

Arg Gln Asp Pro Phe Asp Ser Glu Leu Leu Lys Gln Tyr Gly Ile Arg 
    290                 295                 300 

Thr Gly Thr Gly Ala Ala Thr Gly Ala Leu Ile Gly Leu Gly Leu Asp 
305                 310                 315                 320 

Ile Ala Thr Leu Gly Gly Ser Leu Gly Leu Gly Thr Ala Ile Gly Gly 
                325                 330                 335 

Phe Leu Gly Gly Ile Leu Pro Asn Thr Arg Thr Ile Ser Asp Lys Leu 
            340                 345                 350 

Ala Gly Arg Gln Thr Leu His Thr Asp Pro Glu Thr Leu Thr Leu Leu 
        355                 360                 365 

Ala Ala Arg Ala Leu Asp Leu Leu His Val Leu Gln Thr Arg Gly His 
    370                 375                 380 

Ala Ala Gln Ser Asp Ile Glu Leu His Ser Arg Lys Val Pro Trp Asp 
385                 390                 395                 400 

Ala Ala Arg Leu Pro Pro Glu Leu Asn Lys Ala Arg Ser His Trp Lys 
                405                 410                 415 

Trp Ser Ser Leu Asn Thr His Arg Pro Glu Thr Ser Arg Ala Glu Arg 
            420                 425                 430 

Ala Gly Tyr Val Glu Lys Leu Gln Ile Arg Leu Ser Gly Lys Tyr Ser 
        435                 440                 445 

Glu Leu Asn Leu Asn Gln Asp Lys Ala Ala Ser Arg Arg Gln Tyr Lys 
    450                 455                 460 

 
           
             31  
             2622  
             DNA  
             Neisseria meningitidis  
             
               CDS  
               (1)..(2622)  
               NMB1595  
             
           
            31 

atg aaa acc tcc gaa ctg cgc caa aaa ttc cta aaa ttt ttt gaa acc       48 
Met Lys Thr Ser Glu Leu Arg Gln Lys Phe Leu Lys Phe Phe Glu Thr 
1               5                   10                  15 

aaa ggc cac acc gtc gtc cgc tct tcc agc ctc gtg ccg cac gac gac       96 
Lys Gly His Thr Val Val Arg Ser Ser Ser Leu Val Pro His Asp Asp 
            20                  25                  30 

ccg acc ctg ctg ttt acc aac gcg ggc atg aac cag ttt aaa gac gta      144 
Pro Thr Leu Leu Phe Thr Asn Ala Gly Met Asn Gln Phe Lys Asp Val 
        35                  40                  45 

ttc tta ggt ttc gac aaa cgc ccg tac agc cgc gcc acc acc gcg caa      192 
Phe Leu Gly Phe Asp Lys Arg Pro Tyr Ser Arg Ala Thr Thr Ala Gln 
    50                  55                  60 

aaa tgc gta cgc gca ggc ggc aaa cac aac gac ttg gaa aac gtc ggc      240 
Lys Cys Val Arg Ala Gly Gly Lys His Asn Asp Leu Glu Asn Val Gly 
65                  70                  75                  80 

tac acc gcc cgc cac cac acc ttc ttt gaa atg atg ggc aac ttc tcc      288 
Tyr Thr Ala Arg His His Thr Phe Phe Glu Met Met Gly Asn Phe Ser 
                85                  90                  95 

ttc ggc gac tac ttc aaa cgc gac gcc atc cac ttc gct tgg gaa ttt      336 
Phe Gly Asp Tyr Phe Lys Arg Asp Ala Ile His Phe Ala Trp Glu Phe 
            100                 105                 110 

ctg act tcc ccc gaa tgg ctc aac atc cct aaa gac aaa ctg ttg gcg      384 
Leu Thr Ser Pro Glu Trp Leu Asn Ile Pro Lys Asp Lys Leu Leu Ala 
        115                 120                 125 

acc gtt tac gcg gaa gac gac gaa gcc tac aac atc tgg ttg aac gaa      432 
Thr Val Tyr Ala Glu Asp Asp Glu Ala Tyr Asn Ile Trp Leu Asn Glu 
    130                 135                 140 

atc ggt atg ccg tcc gag cgc atc gtc cgc atc ggc gac aac aaa ggc      480 
Ile Gly Met Pro Ser Glu Arg Ile Val Arg Ile Gly Asp Asn Lys Gly 
145                 150                 155                 160 

gcg aaa tac gca tcc gac aac ttc tgg caa atg ggc gac acc ggc cct      528 
Ala Lys Tyr Ala Ser Asp Asn Phe Trp Gln Met Gly Asp Thr Gly Pro 
                165                 170                 175 

tgc ggc ccc tgc tcc gaa att ttc tac gac cac ggc gaa gaa atc tgg      576 
Cys Gly Pro Cys Ser Glu Ile Phe Tyr Asp His Gly Glu Glu Ile Trp 
            180                 185                 190 

ggc ggc att ccc ggc agt ccc gaa gaa gac ggc gac cgc tgg atc gaa      624 
Gly Gly Ile Pro Gly Ser Pro Glu Glu Asp Gly Asp Arg Trp Ile Glu 
        195                 200                 205 

att tgg aac tgc gta ttt atg cag ttc aac cgc gac gaa caa ggc aat      672 
Ile Trp Asn Cys Val Phe Met Gln Phe Asn Arg Asp Glu Gln Gly Asn 
    210                 215                 220 

atg aac ccg ctt ccc aaa cct tcc gtc gat acc ggt atg ggc ttg gaa      720 
Met Asn Pro Leu Pro Lys Pro Ser Val Asp Thr Gly Met Gly Leu Glu 
225                 230                 235                 240 

cgc ata gcc gcc gtc atg cag cat gtt cac agc aac tac gaa atc gac      768 
Arg Ile Ala Ala Val Met Gln His Val His Ser Asn Tyr Glu Ile Asp 
                245                 250                 255 

ttg ttc caa gac ctg ctc aaa gcc gtt gcc cgc gaa acc ggc gcg ccg      816 
Leu Phe Gln Asp Leu Leu Lys Ala Val Ala Arg Glu Thr Gly Ala Pro 
            260                 265                 270 

ttc aga atg gaa gaa ccc agc ctg aaa gtc atc gcc gac cac atc cgc      864 
Phe Arg Met Glu Glu Pro Ser Leu Lys Val Ile Ala Asp His Ile Arg 
        275                 280                 285 

tcc tgc tcg ttc ctg att gca gac ggc gtc ttg cct tcc aac gaa ggc      912 
Ser Cys Ser Phe Leu Ile Ala Asp Gly Val Leu Pro Ser Asn Glu Gly 
    290                 295                 300 

cgc ggc tac gta ttg cgc cgc att atc cgc cgc gcc gtg cgc cac ggt      960 
Arg Gly Tyr Val Leu Arg Arg Ile Ile Arg Arg Ala Val Arg His Gly 
305                 310                 315                 320 

tac aaa ctg ggt caa agc aaa ccg ttc ttc cac aaa ctc gtt gcc gat     1008 
Tyr Lys Leu Gly Gln Ser Lys Pro Phe Phe His Lys Leu Val Ala Asp 
                325                 330                 335 

ttg gtc aaa gag atg ggc ggt gcc tac cct gaa ttg aaa gaa aaa caa     1056 
Leu Val Lys Glu Met Gly Gly Ala Tyr Pro Glu Leu Lys Glu Lys Gln 
            340                 345                 350 

gcc caa atc gaa gaa gca ttg aaa aac gaa gaa agc cgt ttt gcc caa     1104 
Ala Gln Ile Glu Glu Ala Leu Lys Asn Glu Glu Ser Arg Phe Ala Gln 
        355                 360                 365 

acg ctg gaa acc ggt atg gct ttg ttg gaa aac gcg ctg gtc aaa ggc     1152 
Thr Leu Glu Thr Gly Met Ala Leu Leu Glu Asn Ala Leu Val Lys Gly 
    370                 375                 380 

ggc aaa aca ctc ggc ggc gaa atc atc ttc aaa ctc tac gat acc tac     1200 
Gly Lys Thr Leu Gly Gly Glu Ile Ile Phe Lys Leu Tyr Asp Thr Tyr 
385                 390                 395                 400 

ggt ttc cca tac gac ttg act gcc gac atc tgc cgc gaa cgc aat atc     1248 
Gly Phe Pro Tyr Asp Leu Thr Ala Asp Ile Cys Arg Glu Arg Asn Ile 
                405                 410                 415 

gaa ccg gac gaa gca ggc ttc gag cgc gaa atg gaa gcc caa cgc gca     1296 
Glu Pro Asp Glu Ala Gly Phe Glu Arg Glu Met Glu Ala Gln Arg Ala 
            420                 425                 430 

cgc gca cgc gcc gcc caa agc ttc aaa gcc aac gcc caa ctg cct tat     1344 
Arg Ala Arg Ala Ala Gln Ser Phe Lys Ala Asn Ala Gln Leu Pro Tyr 
        435                 440                 445 

gac ggt caa gac acc gag ttt aaa ggt tat agc gaa cgc caa acc gaa     1392 
Asp Gly Gln Asp Thr Glu Phe Lys Gly Tyr Ser Glu Arg Gln Thr Glu 
    450                 455                 460 

tcc aaa gtc ctc gcc ctc tac aaa gac ggc gag caa gtc aac gaa ttg     1440 
Ser Lys Val Leu Ala Leu Tyr Lys Asp Gly Glu Gln Val Asn Glu Leu 
465                 470                 475                 480 

aac gaa ggc gac agc ggc gca gtc gtc atc gac ttt acc ccg ttc tat     1488 
Asn Glu Gly Asp Ser Gly Ala Val Val Ile Asp Phe Thr Pro Phe Tyr 
                485                 490                 495 

gca gaa tcc ggc ggc caa gtc ggc gat gtc ggc tat atc ttc tca ggc     1536 
Ala Glu Ser Gly Gly Gln Val Gly Asp Val Gly Tyr Ile Phe Ser Gly 
            500                 505                 510 

gaa aac cgc ttt gaa gta cgc gat acc caa aaa atc aaa gcg gcc gta     1584 
Glu Asn Arg Phe Glu Val Arg Asp Thr Gln Lys Ile Lys Ala Ala Val 
        515                 520                 525 

ttc ggt caa ttc ggc gta caa act tca ggc cgt ctg aaa gtc ggc gac     1632 
Phe Gly Gln Phe Gly Val Gln Thr Ser Gly Arg Leu Lys Val Gly Asp 
    530                 535                 540 

agc gtt acc gcc aaa gtg gac gac gaa atc cgc aat gcc aat atg cgc     1680 
Ser Val Thr Ala Lys Val Asp Asp Glu Ile Arg Asn Ala Asn Met Arg 
545                 550                 555                 560 

aac cac agc gca acc cac ttg atg cac aaa gcc ctg cgc gat gta ttg     1728 
Asn His Ser Ala Thr His Leu Met His Lys Ala Leu Arg Asp Val Leu 
                565                 570                 575 

ggc aga cac gtc gaa caa aaa ggc tct ttg gtt acc gcc gaa tcc acc     1776 
Gly Arg His Val Glu Gln Lys Gly Ser Leu Val Thr Ala Glu Ser Thr 
            580                 585                 590 

cgt ttc gac att tcc cat ccc caa gcg gta act gcc gaa gaa att gcc     1824 
Arg Phe Asp Ile Ser His Pro Gln Ala Val Thr Ala Glu Glu Ile Ala 
        595                 600                 605 

gaa gta gaa cgc cgc gtc aac gaa gcc att ttg gcg aac gtt gcc gtc     1872 
Glu Val Glu Arg Arg Val Asn Glu Ala Ile Leu Ala Asn Val Ala Val 
    610                 615                 620 

aat gca gcc att atg agc atg gaa gac gcg caa aaa acc ggc gcg atg     1920 
Asn Ala Ala Ile Met Ser Met Glu Asp Ala Gln Lys Thr Gly Ala Met 
625                 630                 635                 640 

atg ctc ttc ggc gaa aaa tac ggc gaa gaa gtg cgc gta ctg caa atg     1968 
Met Leu Phe Gly Glu Lys Tyr Gly Glu Glu Val Arg Val Leu Gln Met 
                645                 650                 655 

ggc ggt ttc tct acc gaa ttg tgc ggc ggc aca cac gtt tca cgc acc     2016 
Gly Gly Phe Ser Thr Glu Leu Cys Gly Gly Thr His Val Ser Arg Thr 
            660                 665                 670 

ggc gac atc ggc ctc ttc aaa atc atc agc gaa ggc ggt att gcc gca     2064 
Gly Asp Ile Gly Leu Phe Lys Ile Ile Ser Glu Gly Gly Ile Ala Ala 
        675                 680                 685 

ggc gtg cgc cgt atc gaa gcc atc acc ggc ctg aac gca ctc aaa tgg     2112 
Gly Val Arg Arg Ile Glu Ala Ile Thr Gly Leu Asn Ala Leu Lys Trp 
    690                 695                 700 

gcg caa gag caa gag cgt ttg gtg aaa gac att att gcc gaa acc aaa     2160 
Ala Gln Glu Gln Glu Arg Leu Val Lys Asp Ile Ile Ala Glu Thr Lys 
705                 710                 715                 720 

gcc caa acc gaa aaa gac gta ctg gca aaa atc caa gca ggc gcg gca     2208 
Ala Gln Thr Glu Lys Asp Val Leu Ala Lys Ile Gln Ala Gly Ala Ala 
                725                 730                 735 

cac gcc aaa gca ttg gaa aaa gaa ttg gca cgc gcc aaa gcc gaa ctc     2256 
His Ala Lys Ala Leu Glu Lys Glu Leu Ala Arg Ala Lys Ala Glu Leu 
            740                 745                 750 

gcc gtc cac gca ggc gcc aaa ctc ttg gac gat gca aaa gac ttg ggc     2304 
Ala Val His Ala Gly Ala Lys Leu Leu Asp Asp Ala Lys Asp Leu Gly 
        755                 760                 765 

gca gcc aaa ctc gtt gcc gcc caa atc gaa gcc gac gca gcc gcc ctg     2352 
Ala Ala Lys Leu Val Ala Ala Gln Ile Glu Ala Asp Ala Ala Ala Leu 
    770                 775                 780 

cgc gaa atc gtt acc gat tta acc ggt aaa tcc gac aac gcc gtg att     2400 
Arg Glu Ile Val Thr Asp Leu Thr Gly Lys Ser Asp Asn Ala Val Ile 
785                 790                 795                 800 

ctt tta gcg gca gta aac gac ggc aaa gtc tcc ctg tgc gcc ggc gta     2448 
Leu Leu Ala Ala Val Asn Asp Gly Lys Val Ser Leu Cys Ala Gly Val 
                805                 810                 815 

tcc aaa ccg ttg acc ggc aaa gtg aaa gca ggc gat ctg gtt aaa ttt     2496 
Ser Lys Pro Leu Thr Gly Lys Val Lys Ala Gly Asp Leu Val Lys Phe 
            820                 825                 830 

gca gcc gaa caa gtc ggc ggc aaa ggc ggc ggc aga cca gat ttg gcg     2544 
Ala Ala Glu Gln Val Gly Gly Lys Gly Gly Gly Arg Pro Asp Leu Ala 
        835                 840                 845 

caa gcc ggc ggc acg gat gcc gac aaa ttg ccc gcc gtg ttg gat agc     2592 
Gln Ala Gly Gly Thr Asp Ala Asp Lys Leu Pro Ala Val Leu Asp Ser 
    850                 855                 860 

gtg aaa gac tgg gtc ggc gcg aag ctg gtt                             2622 
Val Lys Asp Trp Val Gly Ala Lys Leu Val 
865                 870 

 
           
             32  
             874  
             PRT  
             Neisseria meningitidis  
           
            32 

Met Lys Thr Ser Glu Leu Arg Gln Lys Phe Leu Lys Phe Phe Glu Thr 
1               5                   10                  15 

Lys Gly His Thr Val Val Arg Ser Ser Ser Leu Val Pro His Asp Asp 
            20                  25                  30 

Pro Thr Leu Leu Phe Thr Asn Ala Gly Met Asn Gln Phe Lys Asp Val 
        35                  40                  45 

Phe Leu Gly Phe Asp Lys Arg Pro Tyr Ser Arg Ala Thr Thr Ala Gln 
    50                  55                  60 

Lys Cys Val Arg Ala Gly Gly Lys His Asn Asp Leu Glu Asn Val Gly 
65                  70                  75                  80 

Tyr Thr Ala Arg His His Thr Phe Phe Glu Met Met Gly Asn Phe Ser 
                85                  90                  95 

Phe Gly Asp Tyr Phe Lys Arg Asp Ala Ile His Phe Ala Trp Glu Phe 
            100                 105                 110 

Leu Thr Ser Pro Glu Trp Leu Asn Ile Pro Lys Asp Lys Leu Leu Ala 
        115                 120                 125 

Thr Val Tyr Ala Glu Asp Asp Glu Ala Tyr Asn Ile Trp Leu Asn Glu 
    130                 135                 140 

Ile Gly Met Pro Ser Glu Arg Ile Val Arg Ile Gly Asp Asn Lys Gly 
145                 150                 155                 160 

Ala Lys Tyr Ala Ser Asp Asn Phe Trp Gln Met Gly Asp Thr Gly Pro 
                165                 170                 175 

Cys Gly Pro Cys Ser Glu Ile Phe Tyr Asp His Gly Glu Glu Ile Trp 
            180                 185                 190 

Gly Gly Ile Pro Gly Ser Pro Glu Glu Asp Gly Asp Arg Trp Ile Glu 
        195                 200                 205 

Ile Trp Asn Cys Val Phe Met Gln Phe Asn Arg Asp Glu Gln Gly Asn 
    210                 215                 220 

Met Asn Pro Leu Pro Lys Pro Ser Val Asp Thr Gly Met Gly Leu Glu 
225                 230                 235                 240 

Arg Ile Ala Ala Val Met Gln His Val His Ser Asn Tyr Glu Ile Asp 
                245                 250                 255 

Leu Phe Gln Asp Leu Leu Lys Ala Val Ala Arg Glu Thr Gly Ala Pro 
            260                 265                 270 

Phe Arg Met Glu Glu Pro Ser Leu Lys Val Ile Ala Asp His Ile Arg 
        275                 280                 285 

Ser Cys Ser Phe Leu Ile Ala Asp Gly Val Leu Pro Ser Asn Glu Gly 
    290                 295                 300 

Arg Gly Tyr Val Leu Arg Arg Ile Ile Arg Arg Ala Val Arg His Gly 
305                 310                 315                 320 

Tyr Lys Leu Gly Gln Ser Lys Pro Phe Phe His Lys Leu Val Ala Asp 
                325                 330                 335 

Leu Val Lys Glu Met Gly Gly Ala Tyr Pro Glu Leu Lys Glu Lys Gln 
            340                 345                 350 

Ala Gln Ile Glu Glu Ala Leu Lys Asn Glu Glu Ser Arg Phe Ala Gln 
        355                 360                 365 

Thr Leu Glu Thr Gly Met Ala Leu Leu Glu Asn Ala Leu Val Lys Gly 
    370                 375                 380 

Gly Lys Thr Leu Gly Gly Glu Ile Ile Phe Lys Leu Tyr Asp Thr Tyr 
385                 390                 395                 400 

Gly Phe Pro Tyr Asp Leu Thr Ala Asp Ile Cys Arg Glu Arg Asn Ile 
                405                 410                 415 

Glu Pro Asp Glu Ala Gly Phe Glu Arg Glu Met Glu Ala Gln Arg Ala 
            420                 425                 430 

Arg Ala Arg Ala Ala Gln Ser Phe Lys Ala Asn Ala Gln Leu Pro Tyr 
        435                 440                 445 

Asp Gly Gln Asp Thr Glu Phe Lys Gly Tyr Ser Glu Arg Gln Thr Glu 
    450                 455                 460 

Ser Lys Val Leu Ala Leu Tyr Lys Asp Gly Glu Gln Val Asn Glu Leu 
465                 470                 475                 480 

Asn Glu Gly Asp Ser Gly Ala Val Val Ile Asp Phe Thr Pro Phe Tyr 
                485                 490                 495 

Ala Glu Ser Gly Gly Gln Val Gly Asp Val Gly Tyr Ile Phe Ser Gly 
            500                 505                 510 

Glu Asn Arg Phe Glu Val Arg Asp Thr Gln Lys Ile Lys Ala Ala Val 
        515                 520                 525 

Phe Gly Gln Phe Gly Val Gln Thr Ser Gly Arg Leu Lys Val Gly Asp 
    530                 535                 540 

Ser Val Thr Ala Lys Val Asp Asp Glu Ile Arg Asn Ala Asn Met Arg 
545                 550                 555                 560 

Asn His Ser Ala Thr His Leu Met His Lys Ala Leu Arg Asp Val Leu 
                565                 570                 575 

Gly Arg His Val Glu Gln Lys Gly Ser Leu Val Thr Ala Glu Ser Thr 
            580                 585                 590 

Arg Phe Asp Ile Ser His Pro Gln Ala Val Thr Ala Glu Glu Ile Ala 
        595                 600                 605 

Glu Val Glu Arg Arg Val Asn Glu Ala Ile Leu Ala Asn Val Ala Val 
    610                 615                 620 

Asn Ala Ala Ile Met Ser Met Glu Asp Ala Gln Lys Thr Gly Ala Met 
625                 630                 635                 640 

Met Leu Phe Gly Glu Lys Tyr Gly Glu Glu Val Arg Val Leu Gln Met 
                645                 650                 655 

Gly Gly Phe Ser Thr Glu Leu Cys Gly Gly Thr His Val Ser Arg Thr 
            660                 665                 670 

Gly Asp Ile Gly Leu Phe Lys Ile Ile Ser Glu Gly Gly Ile Ala Ala 
        675                 680                 685 

Gly Val Arg Arg Ile Glu Ala Ile Thr Gly Leu Asn Ala Leu Lys Trp 
    690                 695                 700 

Ala Gln Glu Gln Glu Arg Leu Val Lys Asp Ile Ile Ala Glu Thr Lys 
705                 710                 715                 720 

Ala Gln Thr Glu Lys Asp Val Leu Ala Lys Ile Gln Ala Gly Ala Ala 
                725                 730                 735 

His Ala Lys Ala Leu Glu Lys Glu Leu Ala Arg Ala Lys Ala Glu Leu 
            740                 745                 750 

Ala Val His Ala Gly Ala Lys Leu Leu Asp Asp Ala Lys Asp Leu Gly 
        755                 760                 765 

Ala Ala Lys Leu Val Ala Ala Gln Ile Glu Ala Asp Ala Ala Ala Leu 
    770                 775                 780 

Arg Glu Ile Val Thr Asp Leu Thr Gly Lys Ser Asp Asn Ala Val Ile 
785                 790                 795                 800 

Leu Leu Ala Ala Val Asn Asp Gly Lys Val Ser Leu Cys Ala Gly Val 
                805                 810                 815 

Ser Lys Pro Leu Thr Gly Lys Val Lys Ala Gly Asp Leu Val Lys Phe 
            820                 825                 830 

Ala Ala Glu Gln Val Gly Gly Lys Gly Gly Gly Arg Pro Asp Leu Ala 
        835                 840                 845 

Gln Ala Gly Gly Thr Asp Ala Asp Lys Leu Pro Ala Val Leu Asp Ser 
    850                 855                 860 

Val Lys Asp Trp Val Gly Ala Lys Leu Val 
865                 870 

 
           
             33  
             774  
             DNA  
             Neisseria meningitidis  
             
               CDS  
               (1)..(774)  
               NMA0750  
             
           
            33 

atg gct aag aaa ttc gat aaa gac agc ttc agc ggc acg ctg att gtc       48 
Met Ala Lys Lys Phe Asp Lys Asp Ser Phe Ser Gly Thr Leu Ile Val 
1               5                   10                  15 

gtg ttg gcg gtc agc ctg att tgc tcg gtc atc gtt gcc ggt gcg gtc       96 
Val Leu Ala Val Ser Leu Ile Cys Ser Val Ile Val Ala Gly Ala Val 
            20                  25                  30 

gtc ggc ttg aaa ccc atc caa gag aaa caa aaa ctc caa gac aaa caa      144 
Val Gly Leu Lys Pro Ile Gln Glu Lys Gln Lys Leu Gln Asp Lys Gln 
        35                  40                  45 

ggc tat atc ttg agc gta gcc ggt ttg atg gat aag gac acc gac atc      192 
Gly Tyr Ile Leu Ser Val Ala Gly Leu Met Asp Lys Asp Thr Asp Ile 
    50                  55                  60 

ggt aaa act ttt gcc gag cgt atc gag caa cgc gtg gtc gat ttg gcg      240 
Gly Lys Thr Phe Ala Glu Arg Ile Glu Gln Arg Val Val Asp Leu Ala 
65                  70                  75                  80 

acc ggc gaa tat gtg aaa gac gcg ccg aaa gac ttc agt gcg cgc atc      288 
Thr Gly Glu Tyr Val Lys Asp Ala Pro Lys Asp Phe Ser Ala Arg Ile 
                85                  90                  95 

gca ggc aaa gac ccc gcg caa agc atc cgc atc aaa ccc gaa gac gat      336 
Ala Gly Lys Asp Pro Ala Gln Ser Ile Arg Ile Lys Pro Glu Asp Asp 
            100                 105                 110 

ttg gca ggc atc aaa agc cgt gcc aaa tac acc gag gtt tac ttg gta      384 
Leu Ala Gly Ile Lys Ser Arg Ala Lys Tyr Thr Glu Val Tyr Leu Val 
        115                 120                 125 

aaa ggt gaa gac ggc aaa atc ggc caa atc atc ctg cct atg cac ggt      432 
Lys Gly Glu Asp Gly Lys Ile Gly Gln Ile Ile Leu Pro Met His Gly 
    130                 135                 140 

aac ggt ttg tgg tcg gtc atg tac ggc ttt gtc gcc atc caa ccc gac      480 
Asn Gly Leu Trp Ser Val Met Tyr Gly Phe Val Ala Ile Gln Pro Asp 
145                 150                 155                 160 

ggc aac acc atc aac ggc att acc tac tac gag caa ggc gaa acc ccg      528 
Gly Asn Thr Ile Asn Gly Ile Thr Tyr Tyr Glu Gln Gly Glu Thr Pro 
                165                 170                 175 

ggc tta ggc ggc gaa atc ggc aat cct ttg tgg cag caa aaa ttc gtc      576 
Gly Leu Gly Gly Glu Ile Gly Asn Pro Leu Trp Gln Gln Lys Phe Val 
            180                 185                 190 

ggc aaa aaa ctg ttt gac gga caa ggc aaa ctc gcc ctg cac gtc ggc      624 
Gly Lys Lys Leu Phe Asp Gly Gln Gly Lys Leu Ala Leu His Val Gly 
        195                 200                 205 

aaa ggc gcg ggt tcg gac aaa gaa cac ggc gta gat gcc ctc tcc ggc      672 
Lys Gly Ala Gly Ser Asp Lys Glu His Gly Val Asp Ala Leu Ser Gly 
    210                 215                 220 

gcg tcg ctg aca tcc aaa ggc gta caa ggt tcg ttc gcc tac tgg ttc      720 
Ala Ser Leu Thr Ser Lys Gly Val Gln Gly Ser Phe Ala Tyr Trp Phe 
225                 230                 235                 240 

ggc gaa aac ggc tat atc ccc tac ctg aac aaa ttg aaa tca gca gga      768 
Gly Glu Asn Gly Tyr Ile Pro Tyr Leu Asn Lys Leu Lys Ser Ala Gly 
                245                 250                 255 

gcg caa                                                              774 
Ala Gln 

 
           
             34  
             258  
             PRT  
             Neisseria meningitidis  
           
            34 

Met Ala Lys Lys Phe Asp Lys Asp Ser Phe Ser Gly Thr Leu Ile Val 
1               5                   10                  15 

Val Leu Ala Val Ser Leu Ile Cys Ser Val Ile Val Ala Gly Ala Val 
            20                  25                  30 

Val Gly Leu Lys Pro Ile Gln Glu Lys Gln Lys Leu Gln Asp Lys Gln 
        35                  40                  45 

Gly Tyr Ile Leu Ser Val Ala Gly Leu Met Asp Lys Asp Thr Asp Ile 
    50                  55                  60 

Gly Lys Thr Phe Ala Glu Arg Ile Glu Gln Arg Val Val Asp Leu Ala 
65                  70                  75                  80 

Thr Gly Glu Tyr Val Lys Asp Ala Pro Lys Asp Phe Ser Ala Arg Ile 
                85                  90                  95 

Ala Gly Lys Asp Pro Ala Gln Ser Ile Arg Ile Lys Pro Glu Asp Asp 
            100                 105                 110 

Leu Ala Gly Ile Lys Ser Arg Ala Lys Tyr Thr Glu Val Tyr Leu Val 
        115                 120                 125 

Lys Gly Glu Asp Gly Lys Ile Gly Gln Ile Ile Leu Pro Met His Gly 
    130                 135                 140 

Asn Gly Leu Trp Ser Val Met Tyr Gly Phe Val Ala Ile Gln Pro Asp 
145                 150                 155                 160 

Gly Asn Thr Ile Asn Gly Ile Thr Tyr Tyr Glu Gln Gly Glu Thr Pro 
                165                 170                 175 

Gly Leu Gly Gly Glu Ile Gly Asn Pro Leu Trp Gln Gln Lys Phe Val 
            180                 185                 190 

Gly Lys Lys Leu Phe Asp Gly Gln Gly Lys Leu Ala Leu His Val Gly 
        195                 200                 205 

Lys Gly Ala Gly Ser Asp Lys Glu His Gly Val Asp Ala Leu Ser Gly 
    210                 215                 220 

Ala Ser Leu Thr Ser Lys Gly Val Gln Gly Ser Phe Ala Tyr Trp Phe 
225                 230                 235                 240 

Gly Glu Asn Gly Tyr Ile Pro Tyr Leu Asn Lys Leu Lys Ser Ala Gly 
                245                 250                 255 

Ala Gln 

 
           
             35  
             624  
             DNA  
             Neisseria meningitidis  
             
               CDS  
               (1)..(624)  
               NMA0749  
             
           
            35 

atg gct gat atg aaa cgc ttg aaa cat ttg atg ttt tca ccc ttt atc       48 
Met Ala Asp Met Lys Arg Leu Lys His Leu Met Phe Ser Pro Phe Ile 
1               5                   10                  15 

gac aac aac ccg att gcc ttg cag gtt ttg ggt att tgt tcg gct ttg       96 
Asp Asn Asn Pro Ile Ala Leu Gln Val Leu Gly Ile Cys Ser Ala Leu 
            20                  25                  30 

gcg gtt acc acc aaa ctt cag acg gcc atc gtg atg ggt att tcc gta      144 
Ala Val Thr Thr Lys Leu Gln Thr Ala Ile Val Met Gly Ile Ser Val 
        35                  40                  45 

gct ttg gta acc ggt ttt tcc agc ttc ttc att tcg ctg gta cgc aac      192 
Ala Leu Val Thr Gly Phe Ser Ser Phe Phe Ile Ser Leu Val Arg Asn 
    50                  55                  60 

tac atc ccc aac agc atc cgc atc atc gtg caa atg gcg att atc gcg      240 
Tyr Ile Pro Asn Ser Ile Arg Ile Ile Val Gln Met Ala Ile Ile Ala 
65                  70                  75                  80 

tcg ctg gtt acg ctg gtc gac caa ctc ttg cag gca ttt gcc tac gaa      288 
Ser Leu Val Thr Leu Val Asp Gln Leu Leu Gln Ala Phe Ala Tyr Glu 
                85                  90                  95 

ttg tcc aaa cag ctt tcc gta ttc gtc ggt ctg att att acc aac tgt      336 
Leu Ser Lys Gln Leu Ser Val Phe Val Gly Leu Ile Ile Thr Asn Cys 
            100                 105                 110 

atc gtg atg ggc cgc gcc gaa gca ttt gcg atg aaa gag ccg ccg ctg      384 
Ile Val Met Gly Arg Ala Glu Ala Phe Ala Met Lys Glu Pro Pro Leu 
        115                 120                 125 

gaa agc ctg atc gac ggc atc ggc aac ggc gcg ggc tac ggg atg atg      432 
Glu Ser Leu Ile Asp Gly Ile Gly Asn Gly Ala Gly Tyr Gly Met Met 
    130                 135                 140 

ctg ctt gtc gtc gcc acc gtc cgc gaa ctg att ggc tcg ggc aaa ctc      480 
Leu Leu Val Val Ala Thr Val Arg Glu Leu Ile Gly Ser Gly Lys Leu 
145                 150                 155                 160 

ttg ggc tac acc gtt ttc caa acc gtg cag gac ggc ggc tgg tat cag      528 
Leu Gly Tyr Thr Val Phe Gln Thr Val Gln Asp Gly Gly Trp Tyr Gln 
                165                 170                 175 

acc aac ggc ttg ttc ctg ctc gcc ccc agc gcg ttc ttc atc atc ggc      576 
Thr Asn Gly Leu Phe Leu Leu Ala Pro Ser Ala Phe Phe Ile Ile Gly 
            180                 185                 190 

ttt ttg att tgg gga ctg cgt acc tgg aaa ccc gaa cag gcg gag gaa      624 
Phe Leu Ile Trp Gly Leu Arg Thr Trp Lys Pro Glu Gln Ala Glu Glu 
        195                 200                 205 

 
           
             36  
             208  
             PRT  
             Neisseria meningitidis  
           
            36 

Met Ala Asp Met Lys Arg Leu Lys His Leu Met Phe Ser Pro Phe Ile 
1               5                   10                  15 

Asp Asn Asn Pro Ile Ala Leu Gln Val Leu Gly Ile Cys Ser Ala Leu 
            20                  25                  30 

Ala Val Thr Thr Lys Leu Gln Thr Ala Ile Val Met Gly Ile Ser Val 
        35                  40                  45 

Ala Leu Val Thr Gly Phe Ser Ser Phe Phe Ile Ser Leu Val Arg Asn 
    50                  55                  60 

Tyr Ile Pro Asn Ser Ile Arg Ile Ile Val Gln Met Ala Ile Ile Ala 
65                  70                  75                  80 

Ser Leu Val Thr Leu Val Asp Gln Leu Leu Gln Ala Phe Ala Tyr Glu 
                85                  90                  95 

Leu Ser Lys Gln Leu Ser Val Phe Val Gly Leu Ile Ile Thr Asn Cys 
            100                 105                 110 

Ile Val Met Gly Arg Ala Glu Ala Phe Ala Met Lys Glu Pro Pro Leu 
        115                 120                 125 

Glu Ser Leu Ile Asp Gly Ile Gly Asn Gly Ala Gly Tyr Gly Met Met 
    130                 135                 140 

Leu Leu Val Val Ala Thr Val Arg Glu Leu Ile Gly Ser Gly Lys Leu 
145                 150                 155                 160 

Leu Gly Tyr Thr Val Phe Gln Thr Val Gln Asp Gly Gly Trp Tyr Gln 
                165                 170                 175 

Thr Asn Gly Leu Phe Leu Leu Ala Pro Ser Ala Phe Phe Ile Ile Gly 
            180                 185                 190 

Phe Leu Ile Trp Gly Leu Arg Thr Trp Lys Pro Glu Gln Ala Glu Glu 
        195                 200                 205 

 
           
             37  
             630  
             DNA  
             Neisseria meningitidis  
             
               CDS  
               (1)..(630)  
               NMA0387  
             
           
            37 

atg acc cta cgt tac gaa atc ctc tcc gtt acc ccc ttc cgc caa aac       48 
Met Thr Leu Arg Tyr Glu Ile Leu Ser Val Thr Pro Phe Arg Gln Asn 
1               5                   10                  15 

tgc acc ctg att tgg gac gac gaa agc ggc gaa gcc gtc ctg acc gat       96 
Cys Thr Leu Ile Trp Asp Asp Glu Ser Gly Glu Ala Val Leu Thr Asp 
            20                  25                  30 

gtc ggc ggc gac gtg ccg ttc ctg ctg caa gcg ttg gca aac cgc aaa      144 
Val Gly Gly Asp Val Pro Phe Leu Leu Gln Ala Leu Ala Asn Arg Lys 
        35                  40                  45 

ctt acg ctc acg gca atc tgg ctg acg cac ggc cat ctc gat cac gcg      192 
Leu Thr Leu Thr Ala Ile Trp Leu Thr His Gly His Leu Asp His Ala 
    50                  55                  60 

ggc ggc gtg gtc gaa atg ttg aaa acg cat aaa gtc cct gtc ctc ggg      240 
Gly Gly Val Val Glu Met Leu Lys Thr His Lys Val Pro Val Leu Gly 
65                  70                  75                  80 

ccg cat ccg gac gat gaa ttc ctg ctc caa tcg ctg ccg caa acc acc      288 
Pro His Pro Asp Asp Glu Phe Leu Leu Gln Ser Leu Pro Gln Thr Thr 
                85                  90                  95 

gcg caa tac gga ttt cct gtc tcg ccc gcc ttt gcg ccg aac cgt tgg      336 
Ala Gln Tyr Gly Phe Pro Val Ser Pro Ala Phe Ala Pro Asn Arg Trp 
            100                 105                 110 

ctc gaa gaa ggc gaa acg ctc acg gtc gga cgc tat gcc ttt caa gtg      384 
Leu Glu Glu Gly Glu Thr Leu Thr Val Gly Arg Tyr Ala Phe Gln Val 
        115                 120                 125 

ctg cat att ccg ggc cat acg ccg gga cat atc gtc ttt tat tgt gcc      432 
Leu His Ile Pro Gly His Thr Pro Gly His Ile Val Phe Tyr Cys Ala 
    130                 135                 140 

gag gcg gaa ttg ctg att gcg ggc gac gtg ctg ttt tac gaa acc ata      480 
Glu Ala Glu Leu Leu Ile Ala Gly Asp Val Leu Phe Tyr Glu Thr Ile 
145                 150                 155                 160 

ggc aga acc gat ttt ccg cgc ggc aac cac gcc gac tta atc aat aat      528 
Gly Arg Thr Asp Phe Pro Arg Gly Asn His Ala Asp Leu Ile Asn Asn 
                165                 170                 175 

atc cgc aac aaa tta ttc gcc ctc ccc gaa acc gtg caa gtt gtc gcc      576 
Ile Arg Asn Lys Leu Phe Ala Leu Pro Glu Thr Val Gln Val Val Ala 
            180                 185                 190 

gga cac ggg cgt atg act tcc atc gga cac gaa aaa cgg cac aat ccg      624 
Gly His Gly Arg Met Thr Ser Ile Gly His Glu Lys Arg His Asn Pro 
        195                 200                 205 

ttt ttc                                                              630 
Phe Phe 
    210  
           
             40  
             2514  
             PRT  
             Neisseria meningitidis  
           
            40 

Met Asn Lys Thr Leu Tyr Arg Val Ile Phe Asn Arg Lys Arg Gly Ala 
1               5                   10                  15 

Val Val Ala Val Ala Glu Thr Thr Lys Arg Glu Gly Lys Ser Cys Ala 
            20                  25                  30 

Asp Ser Asp Ser Gly Ser Ala His Val Lys Ser Val Pro Phe Gly Thr 
        35                  40                  45 

Thr His Ala Pro Val Cys Arg Ser Asn Ile Phe Ser Phe Ser Leu Leu 
    50                  55                  60 

Gly Phe Ser Leu Cys Leu Ala Val Gly Thr Ala Asn Ile Ala Phe Ala 
65                  70                  75                  80 

Asp Gly Ile Ile Ala Asp Lys Ala Ala Pro Lys Thr Gln Gln Ala Thr 
                85                  90                  95 

Ile Leu Gln Thr Gly Asn Gly Ile Pro Gln Val Asn Ile Gln Thr Pro 
            100                 105                 110 

Thr Ser Ala Gly Val Ser Val Asn Gln Tyr Ala Gln Phe Asp Val Gly 
        115                 120                 125 

Asn Arg Gly Ala Ile Leu Asn Asn Ser Arg Ser Asn Thr Gln Thr Gln 
    130                 135                 140 

Leu Gly Gly Trp Ile Gln Gly Asn Pro Trp Leu Ala Arg Gly Glu Ala 
145                 150                 155                 160 

Arg Val Val Val Asn Gln Ile Asn Ser Ser His Ser Ser Gln Met Asn 
                165                 170                 175 

Gly Tyr Ile Glu Val Gly Gly Arg Arg Ala Glu Val Val Ile Ala Asn 
            180                 185                 190 

Pro Ala Gly Ile Ala Val Asn Gly Gly Gly Phe Ile Asn Ala Ser Arg 
        195                 200                 205 

Ala Thr Leu Thr Thr Gly Gln Pro Gln Tyr Gln Ala Gly Asp Leu Ser 
    210                 215                 220 

Gly Phe Lys Ile Arg Gln Gly Asn Val Val Ile Ala Gly His Gly Leu 
225                 230                 235                 240 

Asp Ala Arg Asp Thr Asp Phe Thr Arg Ile Leu Ser Tyr His Ser Lys 
                245                 250                 255 

Ile Asp Ala Pro Val Trp Gly Gln Asp Val Arg Val Val Ala Gly Gln 
            260                 265                 270 

Asn Asp Val Val Ala Thr Gly Asn Ala His Ser Pro Ile Leu Asn Asn 
        275                 280                 285 

Ala Ala Ala Asn Thr Ser Asn Asn Thr Ala Asn Asn Gly Thr His Ile 
    290                 295                 300 

Pro Leu Phe Ala Ile Asp Thr Gly Lys Leu Gly Gly Met Tyr Ala Asn 
305                 310                 315                 320 

Lys Ile Thr Leu Ile Ser Thr Ala Glu Gln Ala Gly Ile Arg Asn Gln 
                325                 330                 335 

Gly Gln Leu Phe Ala Ser Ser Gly Asn Val Ala Ile Asp Ala Asn Gly 
            340                 345                 350 

Arg Leu Val Asn Ser Gly Thr Met Ala Ala Ala Asn Ala Lys Asp Thr 
        355                 360                 365 

Asp Asn Thr Ala Glu His Lys Val Asn Ile Arg Ser Gln Gly Val Glu 
    370                 375                 380 

Asn Ser Gly Thr Ala Val Ser Gln Gln Gly Thr Gln Ile His Ser Gln 
385                 390                 395                 400 

Ser Ile Gln Asn Thr Gly Thr Leu Leu Ser Ser Gly Glu Ile Leu Ile 
                405                 410                 415 

His Asn Ser Gly Ser Leu Lys Asn Glu Thr Ser Gly Thr Ile Glu Ala 
            420                 425                 430 

Ala Arg Leu Ala Ile Asp Thr Asp Thr Leu Asn Asn Gln Gly Lys Leu 
        435                 440                 445 

Ser Gln Thr Gly Ser Gln Lys Leu His Ile Asp Ala Gln Gly Lys Met 
    450                 455                 460 

Asp Asn Arg Gly Arg Met Gly Leu Gln Asp Thr Ala Pro Thr Ala Ser 
465                 470                 475                 480 

Asn Gly Ser Ser Asn Gln Thr Gly Asn Ser Tyr Asn Ala Ser Phe His 
                485                 490                 495 

Ser Ser Thr Thr Thr Pro Thr Thr Ala Thr Gly Thr Gly Thr Ala Thr 
            500                 505                 510 

Val Ser Ile Ser Asn Ile Thr Ala Pro Thr Phe Ala Asp Gly Thr Ile 
        515                 520                 525 

Arg Thr His Gly Ala Leu Asp Asn Ser Gly Ser Ile Ile Ala Asn Gly 
    530                 535                 540 

Gln Thr Asp Val Ser Ala Gln Gln Gly Leu Asn Asn Ala Gly Gln Ile 
545                 550                 555                 560 

Asp Ile His Gln Leu Asn Ala Lys Gly Ser Ala Phe Asp Asn His Asn 
                565                 570                 575 

Gly Thr Ile Ile Ser Asp Ala Val His Ile Gln Ala Gly Ser Leu Asn 
            580                 585                 590 

Asn Gln Asn Gly Asn Ile Thr Thr Arg Gln Gln Leu Glu Ile Glu Thr 
        595                 600                 605 

Asp Gln Leu Asp Asn Ala His Gly Lys Leu Leu Ser Ala Glu Ile Ala 
    610                 615                 620 

Asp Leu Ala Val Ser Gly Ser Leu Asn Asn Gln Asn Gly Glu Ile Ala 
625                 630                 635                 640 

Thr Asn Gln Gln Leu Ile Ile His Asp Gly Gln Gln Ser Thr Ala Val 
                645                 650                 655 

Ile Asp Asn Thr Asn Gly Thr Ile Gln Ser Gly Arg Asp Val Ala Ile 
            660                 665                 670 

Gln Ala Lys Ser Leu Ser Asn Asn Gly Thr Leu Ala Ala Asp Asn Lys 
        675                 680                 685 

Leu Asp Ile Ala Leu Gln Asp Asp Phe Tyr Val Glu Arg Asn Ile Val 
    690                 695                 700 

Ala Gly Asn Glu Leu Ser Leu Ser Thr Arg Gly Ser Leu Lys Asn Ser 
705                 710                 715                 720 

His Thr Leu Gln Ala Gly Lys Arg Ile Arg Ile Lys Ala Asn Asn Leu 
                725                 730                 735 

Asp Asn Ala Ala Gln Gly Asn Ile Gln Ser Gly Gly Thr Thr Asp Ile 
            740                 745                 750 

Gly Thr Gln His Asn Leu Thr Asn Arg Gly Leu Ile Asp Gly Gln Gln 
        755                 760                 765 

Thr Lys Ile Gln Ala Gly Gln Met Asn Asn Ile Gly Thr Gly Arg Ile 
    770                 775                 780 

Tyr Gly Asp Asn Ile Ala Ile Ala Ala Thr Arg Leu Asp Asn Gln Asp 
785                 790                 795                 800 

Glu Asn Gly Thr Gly Ala Ala Ile Ala Ala Arg Glu Asn Leu Asn Leu 
                805                 810                 815 

Gly Ile Gly Gln Leu Asn Asn Arg Glu Asn Ser Leu Ile Tyr Ser Gly 
            820                 825                 830 

Asn Asp Met Ala Val Gly Gly Ala Leu Asp Thr Asn Gly Gln Ala Thr 
        835                 840                 845 

Gly Lys Ala Gln Arg Ile His Asn Ala Gly Ala Thr Ile Glu Ala Ala 
    850                 855                 860 

Gly Lys Met Arg Leu Gly Val Glu Lys Leu His Asn Thr Asn Glu His 
865                 870                 875                 880 

Leu Lys Thr Gln Leu Val Glu Thr Gly Arg Glu His Ile Val Asp Tyr 
                885                 890                 895 

Glu Ala Phe Gly Arg His Glu Leu Leu Arg Glu Gly Thr Gln His Glu 
            900                 905                 910 

Leu Gly Trp Ser Val Tyr Asn Asp Glu Ser Asp His Leu Arg Thr Pro 
        915                 920                 925 

Asp Gly Ala Ala His Glu Asn Trp His Lys Tyr Asp Tyr Glu Lys Val 
    930                 935                 940 

Thr Gln Lys Thr Gln Val Thr Gln Thr Ala Pro Ala Lys Ile Ile Ser 
945                 950                 955                 960 

Gly Asn Asp Leu Thr Ile Asp Gly Lys Glu Val Phe Asn Thr Asp Ser 
                965                 970                 975 

Gln Ile Ile Ala Gly Gly Asn Leu Ile Val Gln Thr Glu Lys Asp Gly 
            980                 985                 990 

Leu His Asn Glu Gln Thr Phe Gly  Glu Lys Lys Val Phe  Ser Glu Asn 
        995                 1000                 1005 

Gly Lys  Leu His Ser Tyr Trp  Arg Glu Lys His Lys  Gly Arg Asp 
    1010                 1015                 1020 

Ser Thr  Gly His Ser Glu Gln  Asn Tyr Thr Leu Pro  Glu Glu Ile 
    1025                 1030                 1035 

Thr Arg  Asn Ile Ser Leu Gly  Ser Phe Ala Tyr Glu  Ser His Arg 
    1040                 1045                 1050 

Lys Ala  Leu Ser His His Ala  Pro Ser Gln Gly Thr  Glu Leu Pro 
    1055                 1060                 1065 

Gln Ser  Asn Gly Ile Ser Leu  Pro Tyr Thr Ser Asn  Ser Phe Thr 
    1070                 1075                 1080 

Pro Leu  Pro Ser Ser Ser Leu  Tyr Ile Ile Asn Pro  Val Asn Lys 
    1085                 1090                 1095 

Gly Tyr  Leu Val Glu Thr Asp  Pro Arg Phe Ala Asn  Tyr Arg Gln 
    1100                 1105                 1110 

Trp Leu  Gly Ser Asp Tyr Met  Leu Asp Ser Leu Lys  Leu Asp Pro 
    1115                 1120                 1125 

Asn Asn  Leu His Lys Arg Leu  Gly Asp Gly Tyr Tyr  Glu Gln Arg 
    1130                 1135                 1140 

Leu Ile  Asn Glu Gln Ile Ala  Glu Leu Thr Gly His  Arg Arg Leu 
    1145                 1150                 1155 

Asp Gly  Tyr Gln Asn Asp Glu  Glu Gln Phe Lys Ala  Leu Met Asp 
    1160                 1165                 1170 

Asn Gly  Ala Thr Ala Ala Arg  Ser Met Asn Leu Ser  Val Gly Ile 
    1175                 1180                 1185 

Ala Leu  Ser Ala Glu Gln Val  Ala Gln Leu Thr Ser  Asp Ile Val 
    1190                 1195                 1200 

Trp Leu  Val Gln Lys Glu Val  Lys Leu Pro Asp Gly  Gly Thr Gln 
    1205                 1210                 1215 

Thr Val  Leu Val Pro Gln Val  Tyr Val Arg Val Lys  Asn Gly Asp 
    1220                 1225                 1230 

Ile Asp  Gly Lys Gly Ala Leu  Leu Ser Gly Ser Asn  Thr Gln Ile 
    1235                 1240                 1245 

Asn Val  Ser Gly Ser Leu Lys  Asn Ser Gly Thr Ile  Ala Gly Arg 
    1250                 1255                 1260 

Asn Ala  Leu Ile Ile Asn Thr  Asp Thr Leu Asp Asn  Ile Gly Gly 
    1265                 1270                 1275 

Arg Ile  His Ala Gln Lys Ser  Ala Val Thr Ala Thr  Gln Asp Ile 
    1280                 1285                 1290 

Asn Asn  Ile Gly Gly Met Leu  Ser Ala Glu Gln Thr  Leu Leu Leu 
    1295                 1300                 1305 

Asn Ala  Gly Asn Asn Ile Asn  Ser Gln Ser Thr Thr  Ala Ser Ser 
    1310                 1315                 1320 

Gln Asn  Thr Gln Gly Ser Ser  Thr Tyr Leu Asp Arg  Met Ala Gly 
    1325                 1330                 1335 

Ile Tyr  Ile Thr Gly Lys Glu  Lys Gly Val Leu Ala  Ala Gln Ala 
    1340                 1345                 1350 

Gly Lys  Asp Ile Asn Ile Ile  Ala Gly Gln Ile Ser  Asn Gln Ser 
    1355                 1360                 1365 

Glu Gln  Gly Gln Thr Arg Leu  Gln Ala Gly Arg Asp  Ile Asn Leu 
    1370                 1375                 1380 

Asp Thr  Val Gln Thr Ser Lys  His Gln Ala Thr His  Phe Asp Ala 
    1385                 1390                 1395 

Asp Asn  His Val Ile Arg Gly  Ser Thr Asn Glu Val  Gly Ser Ser 
    1400                 1405                 1410 

Ile Gln  Thr Lys Gly Asp Val  Thr Leu Leu Ser Gly  Asn Asn Leu 
    1415                 1420                 1425 

Asn Ala  Lys Ala Ala Glu Val  Ser Ser Ala Asn Gly  Thr Leu Ala 
    1430                 1435                 1440 

Val Ser  Ala Lys Asn Asp Ile  Asn Ile Ser Ala Gly  Ile Asn Thr 
    1445                 1450                 1455 

Thr His  Val Asp Asp Ala Ser  Lys His Thr Gly Arg  Ser Gly Gly 
    1460                 1465                 1470 

Gly Asn  Lys Leu Val Ile Thr  Asp Lys Ala Gln Ser  His His Glu 
    1475                 1480                 1485 

Thr Ala  Gln Ser Ser Thr Phe  Glu Gly Lys Gln Val  Val Leu Gln 
    1490                 1495                 1500 

Ala Gly  Asn Asp Ala Asn Ile  Leu Gly Ser Asn Val  Ile Ser Asp 
    1505                 1510                 1515 

Asn Gly  Thr Gln Ile Gln Ala  Gly Asn His Val Arg  Ile Gly Thr 
    1520                 1525                 1530 

Thr Gln  Thr Gln Ser Gln Ser  Glu Thr Tyr His Gln  Thr Gln Lys 
    1535                 1540                 1545 

Ser Gly  Leu Met Ser Ala Gly  Ile Gly Phe Thr Ile  Gly Ser Lys 
    1550                 1555                 1560 

Thr Asn  Thr Gln Glu Asn Gln  Ser Gln Ser Asn Glu  His Thr Gly 
    1565                 1570                 1575 

Ser Thr  Val Gly Ser Leu Lys  Gly Asp Thr Thr Ile  Val Ala Gly 
    1580                 1585                 1590 

Lys His  Tyr Glu Gln Ile Gly  Ser Thr Val Ser Ser  Pro Glu Gly 
    1595                 1600                 1605 

Asn Asn  Thr Ile Tyr Ala Gln  Ser Ile Asp Ile Gln  Ala Ala His 
    1610                 1615                 1620 

Asn Lys  Leu Asn Ser Asn Thr  Thr Gln Thr Tyr Glu  Gln Lys Gly 
    1625                 1630                 1635 

Leu Thr  Val Ala Phe Ser Ser  Pro Val Thr Asp Leu  Ala Gln Gln 
    1640                 1645                 1650 

Ala Ile  Ala Val Ala Gln Ser  Ser Lys Gln Val Gly  Gln Ser Lys 
    1655                 1660                 1665 

Asn Asp  Arg Val Asn Ala Met  Ala Ala Ala Asn Ala  Gly Trp Gln 
    1670                 1675                 1680 

Ala Tyr  Gln Thr Gly Lys Ser  Ala Gln Asn Leu Ala  Asn Gly Thr 
    1685                 1690                 1695 

Thr Asn  Ala Lys Gln Val Ser  Ile Ser Ile Thr Tyr  Gly Glu Gln 
    1700                 1705                 1710 

Gln Asn  Arg Gln Thr Thr Gln  Val Gln Ala Asn Gln  Ala Gln Ala 
    1715                 1720                 1725 

Ser Gln  Ile Gln Ala Gly Gly  Lys Thr Thr Leu Ile  Ala Thr Gly 
    1730                 1735                 1740 

Ala Ala  Glu Gln Ser Asn Ile  Asn Ile Ala Gly Ser  Asp Val Ala 
    1745                 1750                 1755 

Gly Lys  Ala Gly Thr Ile Leu  Ile Ala Asp Asn Asp  Ile Thr Leu 
    1760                 1765                 1770 

Gln Ser  Ala Glu Gln Ser Asn  Thr Glu Arg Gly Gln  Asn Lys Ser 
    1775                 1780                 1785 

Ala Gly  Trp Asn Ala Gly Ala  Ala Val Ser Phe Gly  Gln Gly Gly 
    1790                 1795                 1800 

Trp Ser  Leu Gly Val Thr Ala  Gly Gly Asn Val Gly  Lys Gly Tyr 
    1805                 1810                 1815 

Gly Asn  Gly Asp Ser Ile Thr  His Arg His Ser His  Ile Gly Asp 
    1820                 1825                 1830 

Lys Gly  Ser Gln Thr Leu Ile  Gln Ser Gly Gly Asp  Thr Thr Ile 
    1835                 1840                 1845 

Lys Gly  Ala Gln Val Arg Gly  Lys Gly Val Gln Val  Asn Ala Lys 
    1850                 1855                 1860 

Asn Leu  Ser Ile Gln Ser Val  Gln Asp Arg Glu Thr  Tyr Gln Ser 
    1865                 1870                 1875 

Lys Gln  Gln Asn Ala Ser Ala  Gln Val Thr Val Gly  Tyr Gly Phe 
    1880                 1885                 1890 

Ser Ala  Gly Gly Asp Tyr Ser  Gln Ser Lys Ile Arg  Ala Asp His 
    1895                 1900                 1905 

Val Ser  Val Thr Glu Gln Ser  Gly Ile Tyr Ala Gly  Glu Asp Gly 
    1910                 1915                 1920 

Tyr Gln  Ile Lys Val Gly Asn  His Thr Asp Leu Lys  Gly Gly Ile 
    1925                 1930                 1935 

Ile Thr  Ser Thr Gln Ser Ala  Glu Asp Lys Gly Lys  Asn Arg Phe 
    1940                 1945                 1950 

Gln Thr  Ala Thr Leu Thr His  Ser Asp Ile Lys Asn  His Ser Gln 
    1955                 1960                 1965 

Tyr Lys  Gly Glu Ser Phe Gly  Leu Gly Ala Ser Ala  Ser Ile Ser 
    1970                 1975                 1980 

Gly Lys  Thr Leu Gly Gln Gly  Ala Gln Asn Lys Pro  Gln Asn Lys 
    1985                 1990                 1995 

His Leu  Thr Ser Val Ala Asp  Lys Asn Ser Ala Ser  Ser Ser Val 
    2000                 2005                 2010 

Gly Tyr  Gly Ser Asp Ser Asp  Ser Gln Ser Ser Ile  Thr Lys Ser 
    2015                 2020                 2025 

Gly Ile  Asn Thr Arg Asn Ile  Gln Ile Thr Asp Glu  Ala Ala Gln 
    2030                 2035                 2040 

Ile Arg  Leu Thr Gly Lys Thr  Ala Ala Gln Thr Lys  Ala Asp Ile 
    2045                 2050                 2055 

Asp Thr  Asn Val Thr Thr Asp  Thr Ala Glu Arg His  Ser Gly Ser 
    2060                 2065                 2070 

Leu Lys  Asn Thr Phe Asn Lys  Glu Ala Val Gln Ser  Glu Leu Asp 
    2075                 2080                 2085 

Leu Gln  Arg Thr Val Ser Gln  Asp Phe Ser Lys Asn  Val Gln Gln 
    2090                 2095                 2100 

Ala Asn  Thr Glu Ile Asn Gln  His Leu Asp Lys Leu  Lys Ala Asp 
    2105                 2110                 2115 

Lys Glu  Ala Ala Glu Thr Ala  Ala Ala Glu Ala Leu  Ala Asn Gly 
    2120                 2125                 2130 

Asp Met  Glu Thr Ala Lys Arg  Lys Ala His Glu Ala  Gln Asp Ala 
    2135                 2140                 2145 

Ala Ala  Lys Ala Asp Asn Trp  Gln Gln Gly Lys Val  Ile Leu Asn 
    2150                 2155                 2160 

Met Leu  Ala Ser Gly Leu Ala  Ala Pro Thr Gln Ser  Gly Ala Gly 
    2165                 2170                 2175 

Ile Ala  Ala Ala Thr Ala Ser  Pro Ala Val Ser Tyr  Ala Ile Gly 
    2180                 2185                 2190 

Gln His  Phe Lys Asp Leu Ala  Gly Gln Asn Ala Asn  Gly Lys Leu 
    2195                 2200                 2205 

Thr Ala  Ser Gln Glu Thr Ala  His Val Leu Ala His  Ala Val Leu 
    2210                 2215                 2220 

Gly Ala  Ala Val Ala Ala Val  Gly Asp Asn Asn Ala  Leu Ala Gly 
    2225                 2230                 2235 

Ala Leu  Ser Ala Gly Gly Ser  Glu Ala Ala Ala Pro  Tyr Ile Ser 
    2240                 2245                 2250 

Lys Trp  Leu Tyr Gly Lys Glu  Lys Gly Ser Asp Leu  Thr Ala Glu 
    2255                 2260                 2265 

Glu Lys  Glu Thr Val Thr Ala  Ile Thr Asn Val Leu  Gly Thr Ala 
    2270                 2275                 2280 

Thr Gly  Ala Ala Val Gly Asn  Ser Ala Thr Asp Ala  Ala Gln Gly 
    2285                 2290                 2295 

Ser Leu  Asn Ala Gln Ser Ala  Val Glu Asn Asn Asp  Thr Val Glu 
    2300                 2305                 2310 

Gln Val  Lys Phe Ala Leu Arg  His Pro Arg Ile Ala  Ile Ala Ile 
    2315                 2320                 2325 

Gly Ser  Val His Lys Asp Pro  Gly Ser Thr Leu Glu  Pro Asn Ile 
    2330                 2335                 2340 

Ser Thr  Ile Ala Ser Thr Phe  Gln Leu Asn Leu Phe  Pro Asn Ser 
    2345                 2350                 2355 

Glu Phe  Gly Gly Glu Gly Gly  Val Gly Asn Ala Phe  Arg His Val 
    2360                 2365                 2370 

Leu Trp  Gln Ala Thr Ile Thr  Arg Glu Phe Gly Lys  Asp Ile Ala 
    2375                 2380                 2385 

Val Lys  Val Gly Asn Ser His  Glu Ser Gly Glu Lys  Ile Asn Tyr 
    2390                 2395                 2400 

Ser Ile  Arg Arg Asn Leu Ser  Leu Asp Lys Ala Asp  Glu Met Ile 
    2405                 2410                 2415 

Asp Gln  Leu Asn Asn Glu Ile  Gly Arg Glu Ile Ala  Leu Asn Thr 
    2420                 2425                 2430 

Asn Arg  Leu Asn Thr Lys Glu  Leu Val Gly Leu Ile  Leu Glu Thr 
    2435                 2440                 2445 

Tyr Lys  Asn Asn Gly Phe Tyr  Gln Ala Glu Arg Asn  Ser Asn Gly 
    2450                 2455                 2460 

Asn Tyr  Asp Val Val Arg Lys  Arg Leu Ser Glu Lys  Asp Tyr Gln 
    2465                 2470                 2475 

Asn Thr  Ser Asn Ile Leu Ile  His Leu Asp Asn Thr  Gly Ala Gly 
    2480                 2485                 2490 

Phe Lys  Ile Gln Gln Arg Arg  Lys Gln Ile Arg Ala  Gln Ile Ser 
    2495                 2500                 2505 

Ala Arg  Gln Trp Arg Arg 
    2510 

 
           
             41  
             1275  
             DNA  
             Neisseria meningitidis  
             
               CDS  
               (1)..(1275)  
               NMB0115  
             
           
            41 

atg cgt agc agc gat att tta att gta gac gac gaa atc ggc atc cgc       48 
Met Arg Ser Ser Asp Ile Leu Ile Val Asp Asp Glu Ile Gly Ile Arg 
1               5                   10                  15 

gac ctg ctg tcg gaa atc ctg cag gac gaa ggt tat tcg gtc gca ttg       96 
Asp Leu Leu Ser Glu Ile Leu Gln Asp Glu Gly Tyr Ser Val Ala Leu 
            20                  25                  30 

gcg gaa aac gcc gaa gag gcg cgc aag ctg cgc cat cag gcg cgc ccc      144 
Ala Glu Asn Ala Glu Glu Ala Arg Lys Leu Arg His Gln Ala Arg Pro 
        35                  40                  45 

gcg atg gtg ctg ctg gat att tgg atg cct gat tgc gac ggc atc acc      192 
Ala Met Val Leu Leu Asp Ile Trp Met Pro Asp Cys Asp Gly Ile Thr 
    50                  55                  60 

ctt ttg aag gag tgg gcg aaa aac ggg cag ctc aat atg ccg gtg gtg      240 
Leu Leu Lys Glu Trp Ala Lys Asn Gly Gln Leu Asn Met Pro Val Val 
65                  70                  75                  80 

atg atg agc ggg cat gcc agc atc gat acc gcc gtg gaa gcc acc aaa      288 
Met Met Ser Gly His Ala Ser Ile Asp Thr Ala Val Glu Ala Thr Lys 
                85                  90                  95 

atc ggc gcg atc gat ttt ttg gaa aaa ccg att tcc ctg caa aag ctg      336 
Ile Gly Ala Ile Asp Phe Leu Glu Lys Pro Ile Ser Leu Gln Lys Leu 
            100                 105                 110 

ctg tct gcc gtc gaa aac gcg ttg aag tac ggt gcg gcg caa acc gaa      384 
Leu Ser Ala Val Glu Asn Ala Leu Lys Tyr Gly Ala Ala Gln Thr Glu 
        115                 120                 125 

acg ggg cct gta ttc gac aag ctg ggc aac agt gcg gcg att cag gaa      432 
Thr Gly Pro Val Phe Asp Lys Leu Gly Asn Ser Ala Ala Ile Gln Glu 
    130                 135                 140 

atg aac cgt gag gta ggg gct gcg gtg aaa tgt gcc tct ccc gta ctt      480 
Met Asn Arg Glu Val Gly Ala Ala Val Lys Cys Ala Ser Pro Val Leu 
145                 150                 155                 160 

ttg acg ggc gag gcg ggt tcg ccg ttt gaa acg gtg gca cgc tat ttc      528 
Leu Thr Gly Glu Ala Gly Ser Pro Phe Glu Thr Val Ala Arg Tyr Phe 
                165                 170                 175 

cat aaa aac ggt acg ccg tgg gtc agc ccg gca agg gtc gaa tat ctg      576 
His Lys Asn Gly Thr Pro Trp Val Ser Pro Ala Arg Val Glu Tyr Leu 
            180                 185                 190 

atc gat atg ccg atg gaa ctg ttg cag aag gcg gag ggc ggc gtt ttg      624 
Ile Asp Met Pro Met Glu Leu Leu Gln Lys Ala Glu Gly Gly Val Leu 
        195                 200                 205 

tat gtc ggc gac atc gcc cag tac agc cgc aac atc caa gcc ggt att      672 
Tyr Val Gly Asp Ile Ala Gln Tyr Ser Arg Asn Ile Gln Ala Gly Ile 
    210                 215                 220 

gcc ttt att gtc gga aag gcg gaa cac cgc cgc gtc agg gtg gtc gca      720 
Ala Phe Ile Val Gly Lys Ala Glu His Arg Arg Val Arg Val Val Ala 
225                 230                 235                 240 

tcg ggc agc agg gcg gca ggt tca gac ggc att gcc tgc gag gaa aag      768 
Ser Gly Ser Arg Ala Ala Gly Ser Asp Gly Ile Ala Cys Glu Glu Lys 
                245                 250                 255 

ctg gcg gaa ctg ctg tcg gaa tcg gtc gtc cgt att ccg ccg ctg cgt      816 
Leu Ala Glu Leu Leu Ser Glu Ser Val Val Arg Ile Pro Pro Leu Arg 
            260                 265                 270 

atg cag cat gaa gac att ccc ttc ctg ata cag ggg att gcc tgc aat      864 
Met Gln His Glu Asp Ile Pro Phe Leu Ile Gln Gly Ile Ala Cys Asn 
        275                 280                 285 

gtg gcg gaa agc caa aag att gcg cct gcc tca ttc agt gaa gag gca      912 
Val Ala Glu Ser Gln Lys Ile Ala Pro Ala Ser Phe Ser Glu Glu Ala 
    290                 295                 300 

ctt gcc gca ttg acc cgt tac gac tgg ccg gga aat ttc gac caa ctg      960 
Leu Ala Ala Leu Thr Arg Tyr Asp Trp Pro Gly Asn Phe Asp Gln Leu 
305                 310                 315                 320 

caa agc gtc gtt gca acg ctg ttg ttg gag gcg gac gga cag gaa atc     1008 
Gln Ser Val Val Ala Thr Leu Leu Leu Glu Ala Asp Gly Gln Glu Ile 
                325                 330                 335 

ggc gca ggg gcg gtt tct tcc ctt ttg ggg cag aat gtg cct gcc gag     1056 
Gly Ala Gly Ala Val Ser Ser Leu Leu Gly Gln Asn Val Pro Ala Glu 
            340                 345                 350 

ggg gcg gaa gat atg gtg ggc ggg ttt aat ttc aac ctg ccc ctg cgc     1104 
Gly Ala Glu Asp Met Val Gly Gly Phe Asn Phe Asn Leu Pro Leu Arg 
        355                 360                 365 

gaa ttg agg gag gag gtg gag cgg cgt tat ttc gag tac cac atc gcc     1152 
Glu Leu Arg Glu Glu Val Glu Arg Arg Tyr Phe Glu Tyr His Ile Ala 
    370                 375                 380 

caa gaa ggt cag aat atg agc caa gtg gcg cag aaa gtt ggt ttg gaa     1200 
Gln Glu Gly Gln Asn Met Ser Gln Val Ala Gln Lys Val Gly Leu Glu 
385                 390                 395                 400 

cgc acg cac ctt tac cgc aaa ctc aaa cag ctc ggc atc ggc gtt tcg     1248 
Arg Thr His Leu Tyr Arg Lys Leu Lys Gln Leu Gly Ile Gly Val Ser 
                405                 410                 415 

cgc cgg gcg ggg gaa aaa acc gaa gaa                                 1275 
Arg Arg Ala Gly Glu Lys Thr Glu Glu 
            420                 425 

 
           
             42  
             425  
             PRT  
             Neisseria meningitidis  
           
            42 

Met Arg Ser Ser Asp Ile Leu Ile Val Asp Asp Glu Ile Gly Ile Arg 
1               5                   10                  15 

Asp Leu Leu Ser Glu Ile Leu Gln Asp Glu Gly Tyr Ser Val Ala Leu 
            20                  25                  30 

Ala Glu Asn Ala Glu Glu Ala Arg Lys Leu Arg His Gln Ala Arg Pro 
        35                  40                  45 

Ala Met Val Leu Leu Asp Ile Trp Met Pro Asp Cys Asp Gly Ile Thr 
    50                  55                  60 

Leu Leu Lys Glu Trp Ala Lys Asn Gly Gln Leu Asn Met Pro Val Val 
65                  70                  75                  80 

Met Met Ser Gly His Ala Ser Ile Asp Thr Ala Val Glu Ala Thr Lys 
                85                  90                  95 

Ile Gly Ala Ile Asp Phe Leu Glu Lys Pro Ile Ser Leu Gln Lys Leu 
            100                 105                 110 

Leu Ser Ala Val Glu Asn Ala Leu Lys Tyr Gly Ala Ala Gln Thr Glu 
        115                 120                 125 

Thr Gly Pro Val Phe Asp Lys Leu Gly Asn Ser Ala Ala Ile Gln Glu 
    130                 135                 140 

Met Asn Arg Glu Val Gly Ala Ala Val Lys Cys Ala Ser Pro Val Leu 
145                 150                 155                 160 

Leu Thr Gly Glu Ala Gly Ser Pro Phe Glu Thr Val Ala Arg Tyr Phe 
                165                 170                 175 

His Lys Asn Gly Thr Pro Trp Val Ser Pro Ala Arg Val Glu Tyr Leu 
            180                 185                 190 

Ile Asp Met Pro Met Glu Leu Leu Gln Lys Ala Glu Gly Gly Val Leu 
        195                 200                 205 

Tyr Val Gly Asp Ile Ala Gln Tyr Ser Arg Asn Ile Gln Ala Gly Ile 
    210                 215                 220 

Ala Phe Ile Val Gly Lys Ala Glu His Arg Arg Val Arg Val Val Ala 
225                 230                 235                 240 

Ser Gly Ser Arg Ala Ala Gly Ser Asp Gly Ile Ala Cys Glu Glu Lys 
                245                 250                 255 

Leu Ala Glu Leu Leu Ser Glu Ser Val Val Arg Ile Pro Pro Leu Arg 
            260                 265                 270 

Met Gln His Glu Asp Ile Pro Phe Leu Ile Gln Gly Ile Ala Cys Asn 
        275                 280                 285 

Val Ala Glu Ser Gln Lys Ile Ala Pro Ala Ser Phe Ser Glu Glu Ala 
    290                 295                 300 

Leu Ala Ala Leu Thr Arg Tyr Asp Trp Pro Gly Asn Phe Asp Gln Leu 
305                 310                 315                 320 

Gln Ser Val Val Ala Thr Leu Leu Leu Glu Ala Asp Gly Gln Glu Ile 
                325                 330                 335 

Gly Ala Gly Ala Val Ser Ser Leu Leu Gly Gln Asn Val Pro Ala Glu 
            340                 345                 350 

Gly Ala Glu Asp Met Val Gly Gly Phe Asn Phe Asn Leu Pro Leu Arg 
        355                 360                 365 

Glu Leu Arg Glu Glu Val Glu Arg Arg Tyr Phe Glu Tyr His Ile Ala 
    370                 375                 380 

Gln Glu Gly Gln Asn Met Ser Gln Val Ala Gln Lys Val Gly Leu Glu 
385                 390                 395                 400 

Arg Thr His Leu Tyr Arg Lys Leu Lys Gln Leu Gly Ile Gly Val Ser 
                405                 410                 415 

Arg Arg Ala Gly Glu Lys Thr Glu Glu 
            420                 425 

 
           
             43  
             1191  
             DNA  
             Neisseria meningitidis  
             
               CDS  
               (1)..(1191)  
               NMB0116  
             
           
            43 

atg aca gag gac gaa cgt ttc gcg tgg ctg caa ttg gcg ttt acg ccc       48 
Met Thr Glu Asp Glu Arg Phe Ala Trp Leu Gln Leu Ala Phe Thr Pro 
1               5                   10                  15 

tat atc ggc gcg gaa agt ttc ctg ctg ctg atg cgc cgt ttc ggc agc       96 
Tyr Ile Gly Ala Glu Ser Phe Leu Leu Leu Met Arg Arg Phe Gly Ser 
            20                  25                  30 

gcg caa aat gcc ctg tcc gca ccg gcg gaa cag gtg gcg gca ctg ata      144 
Ala Gln Asn Ala Leu Ser Ala Pro Ala Glu Gln Val Ala Ala Leu Ile 
        35                  40                  45 

cgg cac aaa cag gcg ctt gag gct tgg cgc aat gcg gaa aaa cgc gct      192 
Arg His Lys Gln Ala Leu Glu Ala Trp Arg Asn Ala Glu Lys Arg Ala 
    50                  55                  60 

ctg gcg cgg cag gcg gca gaa gcg gca ttg gaa tgg gaa atg cgg gac      240 
Leu Ala Arg Gln Ala Ala Glu Ala Ala Leu Glu Trp Glu Met Arg Asp 
65                  70                  75                  80 

gga tgc cgc ctg atg ctg ctt cag gat gaa gat ttt ccc gaa atg ctg      288 
Gly Cys Arg Leu Met Leu Leu Gln Asp Glu Asp Phe Pro Glu Met Leu 
                85                  90                  95 

acg cag ggg ctg acc gcg cca ccg gtt ttg ttt ttg cgc ggc aac gtg      336 
Thr Gln Gly Leu Thr Ala Pro Pro Val Leu Phe Leu Arg Gly Asn Val 
            100                 105                 110 

caa ctg ctg cac aaa cct tcc gcc gcc atc gtc ggc agc cgt cat gcc      384 
Gln Leu Leu His Lys Pro Ser Ala Ala Ile Val Gly Ser Arg His Ala 
        115                 120                 125 

acg ccg cag gcg atg cgg att gcc aaa gat ttc ggc aag tcg ttg ggt      432 
Thr Pro Gln Ala Met Arg Ile Ala Lys Asp Phe Gly Lys Ser Leu Gly 
    130                 135                 140 

ggg aaa ggc att ccc gtt gtg tcg ggt atg gct tcg ggc atc gat acc      480 
Gly Lys Gly Ile Pro Val Val Ser Gly Met Ala Ser Gly Ile Asp Thr 
145                 150                 155                 160 

gcc gcc cat cag ggt gcg ttg cag gca gaa ggc ggc acc atc gcc gtg      528 
Ala Ala His Gln Gly Ala Leu Gln Ala Glu Gly Gly Thr Ile Ala Val 
                165                 170                 175 

tgg ggg acg ggc ata gac cgc att tat ccg ccg gtc aac aaa aac ctt      576 
Trp Gly Thr Gly Ile Asp Arg Ile Tyr Pro Pro Val Asn Lys Asn Leu 
            180                 185                 190 

gcc tat gaa atc gcc gaa aaa gga ttg att gtc agc gag ttc ccc atc      624 
Ala Tyr Glu Ile Ala Glu Lys Gly Leu Ile Val Ser Glu Phe Pro Ile 
        195                 200                 205 

ggc acg cgg ccg tat gcc ggc aat ttt ccg cgc cgc aac cgc ctg att      672 
Gly Thr Arg Pro Tyr Ala Gly Asn Phe Pro Arg Arg Asn Arg Leu Ile 
    210                 215                 220 

gcc gcc ctg tcg caa gta acg ctg gtg gtt gaa gcc gcg ttg gaa tcc      720 
Ala Ala Leu Ser Gln Val Thr Leu Val Val Glu Ala Ala Leu Glu Ser 
225                 230                 235                 240 

ggt tcg ctg att act gcc aga ttg gcg gcg gag atg ggg cgc gaa gtg      768 
Gly Ser Leu Ile Thr Ala Arg Leu Ala Ala Glu Met Gly Arg Glu Val 
                245                 250                 255 

atg gcg gta ccc ggc tcg ata gac aat cca cac agt aaa ggc tgc cac      816 
Met Ala Val Pro Gly Ser Ile Asp Asn Pro His Ser Lys Gly Cys His 
            260                 265                 270 

aaa ctg att aaa gac ggc gca aaa ttg gtg gaa tgc ctg gac gac atc      864 
Lys Leu Ile Lys Asp Gly Ala Lys Leu Val Glu Cys Leu Asp Asp Ile 
        275                 280                 285 

ctg aac gaa tgc ccg ggg cta ttg caa aat acg ggt gct tca tca tat      912 
Leu Asn Glu Cys Pro Gly Leu Leu Gln Asn Thr Gly Ala Ser Ser Tyr 
    290                 295                 300 

tct ata aat aag gga ata cct gaa aag cgc atc act gcc gtt cag acg      960 
Ser Ile Asn Lys Gly Ile Pro Glu Lys Arg Ile Thr Ala Val Gln Thr 
305                 310                 315                 320 

gca tcc gac cag ctg tct ctg cct gaa ggc aaa atg ccg tct gaa aag     1008 
Ala Ser Asp Gln Leu Ser Leu Pro Glu Gly Lys Met Pro Ser Glu Lys 
                325                 330                 335 

acg gag aac cga ccc gtc ggc ggc agt atc ttg gac agg atg ggt ttc     1056 
Thr Glu Asn Arg Pro Val Gly Gly Ser Ile Leu Asp Arg Met Gly Phe 
            340                 345                 350 

gac cca gtt cat ccc gac gtg ctt gcc gga cag ttg gct atg cct gcc     1104 
Asp Pro Val His Pro Asp Val Leu Ala Gly Gln Leu Ala Met Pro Ala 
        355                 360                 365 

gca gat ttg tat gcc gca ctg ttg gaa ttg gaa ttg gac ggc agc gtt     1152 
Ala Asp Leu Tyr Ala Ala Leu Leu Glu Leu Glu Leu Asp Gly Ser Val 
    370                 375                 380 

gcc gca atg ccc ggc ggc aga tac cag cgt atc cga act                 1191 
Ala Ala Met Pro Gly Gly Arg Tyr Gln Arg Ile Arg Thr 
385                 390                 395 

 
           
             44  
             397  
             PRT  
             Neisseria meningitidis  
           
            44 

Met Thr Glu Asp Glu Arg Phe Ala Trp Leu Gln Leu Ala Phe Thr Pro 
1               5                   10                  15 

Tyr Ile Gly Ala Glu Ser Phe Leu Leu Leu Met Arg Arg Phe Gly Ser 
            20                  25                  30 

Ala Gln Asn Ala Leu Ser Ala Pro Ala Glu Gln Val Ala Ala Leu Ile 
        35                  40                  45 

Arg His Lys Gln Ala Leu Glu Ala Trp Arg Asn Ala Glu Lys Arg Ala 
    50                  55                  60 

Leu Ala Arg Gln Ala Ala Glu Ala Ala Leu Glu Trp Glu Met Arg Asp 
65                  70                  75                  80 

Gly Cys Arg Leu Met Leu Leu Gln Asp Glu Asp Phe Pro Glu Met Leu 
                85                  90                  95 

Thr Gln Gly Leu Thr Ala Pro Pro Val Leu Phe Leu Arg Gly Asn Val 
            100                 105                 110 

Gln Leu Leu His Lys Pro Ser Ala Ala Ile Val Gly Ser Arg His Ala 
        115                 120                 125 

Thr Pro Gln Ala Met Arg Ile Ala Lys Asp Phe Gly Lys Ser Leu Gly 
    130                 135                 140 

Gly Lys Gly Ile Pro Val Val Ser Gly Met Ala Ser Gly Ile Asp Thr 
145                 150                 155                 160 

Ala Ala His Gln Gly Ala Leu Gln Ala Glu Gly Gly Thr Ile Ala Val 
                165                 170                 175 

Trp Gly Thr Gly Ile Asp Arg Ile Tyr Pro Pro Val Asn Lys Asn Leu 
            180                 185                 190 

Ala Tyr Glu Ile Ala Glu Lys Gly Leu Ile Val Ser Glu Phe Pro Ile 
        195                 200                 205 

Gly Thr Arg Pro Tyr Ala Gly Asn Phe Pro Arg Arg Asn Arg Leu Ile 
    210                 215                 220 

Ala Ala Leu Ser Gln Val Thr Leu Val Val Glu Ala Ala Leu Glu Ser 
225                 230                 235                 240 

Gly Ser Leu Ile Thr Ala Arg Leu Ala Ala Glu Met Gly Arg Glu Val 
                245                 250                 255 

Met Ala Val Pro Gly Ser Ile Asp Asn Pro His Ser Lys Gly Cys His 
            260                 265                 270 

Lys Leu Ile Lys Asp Gly Ala Lys Leu Val Glu Cys Leu Asp Asp Ile 
        275                 280                 285 

Leu Asn Glu Cys Pro Gly Leu Leu Gln Asn Thr Gly Ala Ser Ser Tyr 
    290                 295                 300 

Ser Ile Asn Lys Gly Ile Pro Glu Lys Arg Ile Thr Ala Val Gln Thr 
305                 310                 315                 320 

Ala Ser Asp Gln Leu Ser Leu Pro Glu Gly Lys Met Pro Ser Glu Lys 
                325                 330                 335 

Thr Glu Asn Arg Pro Val Gly Gly Ser Ile Leu Asp Arg Met Gly Phe 
            340                 345                 350 

Asp Pro Val His Pro Asp Val Leu Ala Gly Gln Leu Ala Met Pro Ala 
        355                 360                 365 

Ala Asp Leu Tyr Ala Ala Leu Leu Glu Leu Glu Leu Asp Gly Ser Val 
    370                 375                 380 

Ala Ala Met Pro Gly Gly Arg Tyr Gln Arg Ile Arg Thr 
385                 390                 395 

 
           
             45  
             459  
             DNA  
             Neisseria meningitidis  
             
               CDS  
               (1)..(459)  
               NMB0117  
             
           
            45 

atg acc gaa gtc atc gcc tac ctc atc gaa cat ttc caa gat ttc gat       48 
Met Thr Glu Val Ile Ala Tyr Leu Ile Glu His Phe Gln Asp Phe Asp 
1               5                   10                  15 

acc tgc ccg ccg ccc gaa gac ttg ggt atg ctg ctt gaa gaa gcg ggt       96 
Thr Cys Pro Pro Pro Glu Asp Leu Gly Met Leu Leu Glu Glu Ala Gly 
            20                  25                  30 

ttc gat acg atg gaa atc ggc aac acc ctg atg atg atg gaa gta ttg      144 
Phe Asp Thr Met Glu Ile Gly Asn Thr Leu Met Met Met Glu Val Leu 
        35                  40                  45 

ctc aac agc tcc gaa ttt tcc gcc gaa ccc gcc gac agc ggc gca ttg      192 
Leu Asn Ser Ser Glu Phe Ser Ala Glu Pro Ala Asp Ser Gly Ala Leu 
    50                  55                  60 

cgc gtg tac agc aaa gaa gaa acc gac aac ctg ccg cag gaa gtg atg      240 
Arg Val Tyr Ser Lys Glu Glu Thr Asp Asn Leu Pro Gln Glu Val Met 
65                  70                  75                  80 

ggg ctg atg cag tat ctg att gaa gaa aaa gcc gtc agc tgc gaa cag      288 
Gly Leu Met Gln Tyr Leu Ile Glu Glu Lys Ala Val Ser Cys Glu Gln 
                85                  90                  95 

cgg gaa atc atc atc cac gcg ctc atg cac att ccg ggc gac gaa att      336 
Arg Glu Ile Ile Ile His Ala Leu Met His Ile Pro Gly Asp Glu Ile 
            100                 105                 110 

acc gta gat acc gcc aaa gtg ctg acc ctg ctg ctt tta tgg gca aac      384 
Thr Val Asp Thr Ala Lys Val Leu Thr Leu Leu Leu Leu Trp Ala Asn 
        115                 120                 125 

aag agc gag ctg ccc gtg ttg gtc ggc gac gag ctg atg agc gcg ctt      432 
Lys Ser Glu Leu Pro Val Leu Val Gly Asp Glu Leu Met Ser Ala Leu 
    130                 135                 140 

tta ctc gac aac aaa ccc acg atg aac                                  459 
Leu Leu Asp Asn Lys Pro Thr Met Asn 
145                 150 

 
           
             46  
             153  
             PRT  
             Neisseria meningitidis  
           
            46 

Met Thr Glu Val Ile Ala Tyr Leu Ile Glu His Phe Gln Asp Phe Asp 
1               5                   10                  15 

Thr Cys Pro Pro Pro Glu Asp Leu Gly Met Leu Leu Glu Glu Ala Gly 
            20                  25                  30 

Phe Asp Thr Met Glu Ile Gly Asn Thr Leu Met Met Met Glu Val Leu 
        35                  40                  45 

Leu Asn Ser Ser Glu Phe Ser Ala Glu Pro Ala Asp Ser Gly Ala Leu 
    50                  55                  60 

Arg Val Tyr Ser Lys Glu Glu Thr Asp Asn Leu Pro Gln Glu Val Met 
65                  70                  75                  80 

Gly Leu Met Gln Tyr Leu Ile Glu Glu Lys Ala Val Ser Cys Glu Gln 
                85                  90                  95 

Arg Glu Ile Ile Ile His Ala Leu Met His Ile Pro Gly Asp Glu Ile 
            100                 105                 110 

Thr Val Asp Thr Ala Lys Val Leu Thr Leu Leu Leu Leu Trp Ala Asn 
        115                 120                 125 

Lys Ser Glu Leu Pro Val Leu Val Gly Asp Glu Leu Met Ser Ala Leu 
    130                 135                 140 

Leu Leu Asp Asn Lys Pro Thr Met Asn 
145                 150 

 
           
             47  
             1776  
             DNA  
             Neisseria meningitidis  
             
               CDS  
               (1)..(1776)  
               NMB2075  
             
           
            47 

atg acg gtt ttg aag ctt tcg cac tgg cgg gtg ttg gcg gag ctt gcc       48 
Met Thr Val Leu Lys Leu Ser His Trp Arg Val Leu Ala Glu Leu Ala 
1               5                   10                  15 

gac ggt ttg ccg caa cac gtc tcg caa ctg gcg cgt atg gcg gat atg       96 
Asp Gly Leu Pro Gln His Val Ser Gln Leu Ala Arg Met Ala Asp Met 
            20                  25                  30 

aag ccg cag cag ctc aac ggt ttt tgg cag cag atg ccg gcg cac ata      144 
Lys Pro Gln Gln Leu Asn Gly Phe Trp Gln Gln Met Pro Ala His Ile 
        35                  40                  45 

cgc ggg ctg ttg cgc caa cac gac ggc tat tgg cgg ctg gtg cgc cca      192 
Arg Gly Leu Leu Arg Gln His Asp Gly Tyr Trp Arg Leu Val Arg Pro 
    50                  55                  60 

ttg gcg gtt ttc gat gcc gaa ggt ttg cgc gag ctg ggg gaa agg tcg      240 
Leu Ala Val Phe Asp Ala Glu Gly Leu Arg Glu Leu Gly Glu Arg Ser 
65                  70                  75                  80 

ggt ttt cag acg gca ttg aag cac gag tgc gcg tcc agc aac gac gag      288 
Gly Phe Gln Thr Ala Leu Lys His Glu Cys Ala Ser Ser Asn Asp Glu 
                85                  90                  95 

ata ctg gaa ttg gcg cgg att gcg ccg gac aag gcg cac aaa acc ata      336 
Ile Leu Glu Leu Ala Arg Ile Ala Pro Asp Lys Ala His Lys Thr Ile 
            100                 105                 110 

tgc gtg acc cac ctg caa agt aag ggc agg ggg cgg cag ggg cgg aag      384 
Cys Val Thr His Leu Gln Ser Lys Gly Arg Gly Arg Gln Gly Arg Lys 
        115                 120                 125 

tgg tcg cac cgt ttg ggc gag tgt ctg atg ttc agt ttt ggc tgg gtg      432 
Trp Ser His Arg Leu Gly Glu Cys Leu Met Phe Ser Phe Gly Trp Val 
    130                 135                 140 

ttt gac cgg ccg cag tat gag ttg ggt tcg ctg tcg cct gtt gcg gca      480 
Phe Asp Arg Pro Gln Tyr Glu Leu Gly Ser Leu Ser Pro Val Ala Ala 
145                 150                 155                 160 

gtg gcg tgt cgg cgc gcc ttg tcg cgt tta ggt ttg gat gtg cag att      528 
Val Ala Cys Arg Arg Ala Leu Ser Arg Leu Gly Leu Asp Val Gln Ile 
                165                 170                 175 

aag tgg ccc aat gat ttg gtt gtc gga cgc gac aaa ttg ggc ggc att      576 
Lys Trp Pro Asn Asp Leu Val Val Gly Arg Asp Lys Leu Gly Gly Ile 
            180                 185                 190 

ctg att gaa acg gtc agg acg ggc ggc aaa acg gtt gcc gtg gtc ggt      624 
Leu Ile Glu Thr Val Arg Thr Gly Gly Lys Thr Val Ala Val Val Gly 
        195                 200                 205 

atc ggc atc aat ttt gtc ctg ccc aag gaa gta gaa aat gcc gct tcc      672 
Ile Gly Ile Asn Phe Val Leu Pro Lys Glu Val Glu Asn Ala Ala Ser 
    210                 215                 220 

gtg caa tcg ctg ttt cag acg gca tcg cgg cgg ggc aat gcc gat gcc      720 
Val Gln Ser Leu Phe Gln Thr Ala Ser Arg Arg Gly Asn Ala Asp Ala 
225                 230                 235                 240 

gcc gtg ctg ctg gaa acg ctg ttg gtg gaa ctg gac gcg gtg ttg ttg      768 
Ala Val Leu Leu Glu Thr Leu Leu Val Glu Leu Asp Ala Val Leu Leu 
                245                 250                 255 

caa tat gcg cgg gac gga ttt gcg cct ttt gtg gcg gaa tat cag gct      816 
Gln Tyr Ala Arg Asp Gly Phe Ala Pro Phe Val Ala Glu Tyr Gln Ala 
            260                 265                 270 

gcc aac cgc gac cac ggc aag gcg gta ttg ctg ttg cgc gac ggc gaa      864 
Ala Asn Arg Asp His Gly Lys Ala Val Leu Leu Leu Arg Asp Gly Glu 
        275                 280                 285 

acc gtg ttc gaa ggc acg gtt aaa ggc gtg gac gga caa ggc gtt ttg      912 
Thr Val Phe Glu Gly Thr Val Lys Gly Val Asp Gly Gln Gly Val Leu 
    290                 295                 300 

cac ttg gaa acg gca gag ggc aaa cag acg gtc gtc agc ggc gaa atc      960 
His Leu Glu Thr Ala Glu Gly Lys Gln Thr Val Val Ser Gly Glu Ile 
305                 310                 315                 320 

agc ctg cgg tcc gac gac agg ccg gtt tcc gtg ccg aag cgg cgg gat     1008 
Ser Leu Arg Ser Asp Asp Arg Pro Val Ser Val Pro Lys Arg Arg Asp 
                325                 330                 335 

tcg gaa cgt ttt ctg ctg ttg gac ggc ggc aac agc cgg ctc aag tgg     1056 
Ser Glu Arg Phe Leu Leu Leu Asp Gly Gly Asn Ser Arg Leu Lys Trp 
            340                 345                 350 

gcg tgg gtg gaa aac ggc acg ttc gca acc gtc ggt agc gcg ccg tac     1104 
Ala Trp Val Glu Asn Gly Thr Phe Ala Thr Val Gly Ser Ala Pro Tyr 
        355                 360                 365 

cgc gat ttg tcg cct ttg ggc gcg gag tgg gcg gaa aag gcg gat gga     1152 
Arg Asp Leu Ser Pro Leu Gly Ala Glu Trp Ala Glu Lys Ala Asp Gly 
    370                 375                 380 

aat gtc cgc atc gtc ggt tgc gct gtg tgc gga gaa ttc aaa aag gca     1200 
Asn Val Arg Ile Val Gly Cys Ala Val Cys Gly Glu Phe Lys Lys Ala 
385                 390                 395                 400 

caa gtg cag gaa cag ctc gcc cga aaa atc gag tgg ctg ccg tct tcc     1248 
Gln Val Gln Glu Gln Leu Ala Arg Lys Ile Glu Trp Leu Pro Ser Ser 
                405                 410                 415 

gca cag gct ttg ggc ata cgc aac cac tac cgc cac ccc gaa gaa cac     1296 
Ala Gln Ala Leu Gly Ile Arg Asn His Tyr Arg His Pro Glu Glu His 
            420                 425                 430 

ggt tcc gac cgc tgg ttc aac gcc ttg ggc agc cgc cgc ttc agc cgc     1344 
Gly Ser Asp Arg Trp Phe Asn Ala Leu Gly Ser Arg Arg Phe Ser Arg 
        435                 440                 445 

aac gcc tgc gtc gtc gtc agt tgc ggc acg gcg gta acg gtt gac gcg     1392 
Asn Ala Cys Val Val Val Ser Cys Gly Thr Ala Val Thr Val Asp Ala 
    450                 455                 460 

ctc acc gat gac gga cat tat ctc ggg gga acc atc atg ccc ggt ttc     1440 
Leu Thr Asp Asp Gly His Tyr Leu Gly Gly Thr Ile Met Pro Gly Phe 
465                 470                 475                 480 

cac ctg atg aaa gaa tcg ctc gcc gtc cga acc gcc aac ctc aac cgg     1488 
His Leu Met Lys Glu Ser Leu Ala Val Arg Thr Ala Asn Leu Asn Arg 
                485                 490                 495 

cac gcc ggt aag cgt tat cct ttc ccg acc aca acg ggc aat gcc gtc     1536 
His Ala Gly Lys Arg Tyr Pro Phe Pro Thr Thr Thr Gly Asn Ala Val 
            500                 505                 510 

gcc agc ggc atg atg gat gcg gtt tgc ggc tcg gtt atg atg atg cac     1584 
Ala Ser Gly Met Met Asp Ala Val Cys Gly Ser Val Met Met Met His 
        515                 520                 525 

ggg cgt ttg aaa gaa aaa acc ggg gcg ggc aag cct gtc gat gtc atc     1632 
Gly Arg Leu Lys Glu Lys Thr Gly Ala Gly Lys Pro Val Asp Val Ile 
    530                 535                 540 

att acc ggc ggc ggc gcg gca aaa gtt gcc gaa gcc ctg ccg cct gca     1680 
Ile Thr Gly Gly Gly Ala Ala Lys Val Ala Glu Ala Leu Pro Pro Ala 
545                 550                 555                 560 

ttt ttg gcg gaa aat acc gtg cgc gtg gcg gac aac ctc gtc att tac     1728 
Phe Leu Ala Glu Asn Thr Val Arg Val Ala Asp Asn Leu Val Ile Tyr 
                565                 570                 575 

ggg ttg ttg aac atg att gcc gcc gaa ggc agg gaa tat gaa cat att     1776 
Gly Leu Leu Asn Met Ile Ala Ala Glu Gly Arg Glu Tyr Glu His Ile 
            580                 585                 590 

 
           
             48  
             592  
             PRT  
             Neisseria meningitidis  
           
            48 

Met Thr Val Leu Lys Leu Ser His Trp Arg Val Leu Ala Glu Leu Ala 
1               5                   10                  15 

Asp Gly Leu Pro Gln His Val Ser Gln Leu Ala Arg Met Ala Asp Met 
            20                  25                  30 

Lys Pro Gln Gln Leu Asn Gly Phe Trp Gln Gln Met Pro Ala His Ile 
        35                  40                  45 

Arg Gly Leu Leu Arg Gln His Asp Gly Tyr Trp Arg Leu Val Arg Pro 
    50                  55                  60 

Leu Ala Val Phe Asp Ala Glu Gly Leu Arg Glu Leu Gly Glu Arg Ser 
65                  70                  75                  80 

Gly Phe Gln Thr Ala Leu Lys His Glu Cys Ala Ser Ser Asn Asp Glu 
                85                  90                  95 

Ile Leu Glu Leu Ala Arg Ile Ala Pro Asp Lys Ala His Lys Thr Ile 
            100                 105                 110 

Cys Val Thr His Leu Gln Ser Lys Gly Arg Gly Arg Gln Gly Arg Lys 
        115                 120                 125 

Trp Ser His Arg Leu Gly Glu Cys Leu Met Phe Ser Phe Gly Trp Val 
    130                 135                 140 

Phe Asp Arg Pro Gln Tyr Glu Leu Gly Ser Leu Ser Pro Val Ala Ala 
145                 150                 155                 160 

Val Ala Cys Arg Arg Ala Leu Ser Arg Leu Gly Leu Asp Val Gln Ile 
                165                 170                 175 

Lys Trp Pro Asn Asp Leu Val Val Gly Arg Asp Lys Leu Gly Gly Ile 
            180                 185                 190 

Leu Ile Glu Thr Val Arg Thr Gly Gly Lys Thr Val Ala Val Val Gly 
        195                 200                 205 

Ile Gly Ile Asn Phe Val Leu Pro Lys Glu Val Glu Asn Ala Ala Ser 
    210                 215                 220 

Val Gln Ser Leu Phe Gln Thr Ala Ser Arg Arg Gly Asn Ala Asp Ala 
225                 230                 235                 240 

Ala Val Leu Leu Glu Thr Leu Leu Val Glu Leu Asp Ala Val Leu Leu 
                245                 250                 255 

Gln Tyr Ala Arg Asp Gly Phe Ala Pro Phe Val Ala Glu Tyr Gln Ala 
            260                 265                 270 

Ala Asn Arg Asp His Gly Lys Ala Val Leu Leu Leu Arg Asp Gly Glu 
        275                 280                 285 

Thr Val Phe Glu Gly Thr Val Lys Gly Val Asp Gly Gln Gly Val Leu 
    290                 295                 300 

His Leu Glu Thr Ala Glu Gly Lys Gln Thr Val Val Ser Gly Glu Ile 
305                 310                 315                 320 

Ser Leu Arg Ser Asp Asp Arg Pro Val Ser Val Pro Lys Arg Arg Asp 
                325                 330                 335 

Ser Glu Arg Phe Leu Leu Leu Asp Gly Gly Asn Ser Arg Leu Lys Trp 
            340                 345                 350 

Ala Trp Val Glu Asn Gly Thr Phe Ala Thr Val Gly Ser Ala Pro Tyr 
        355                 360                 365 

Arg Asp Leu Ser Pro Leu Gly Ala Glu Trp Ala Glu Lys Ala Asp Gly 
    370                 375                 380 

Asn Val Arg Ile Val Gly Cys Ala Val Cys Gly Glu Phe Lys Lys Ala 
385                 390                 395                 400 

Gln Val Gln Glu Gln Leu Ala Arg Lys Ile Glu Trp Leu Pro Ser Ser 
                405                 410                 415 

Ala Gln Ala Leu Gly Ile Arg Asn His Tyr Arg His Pro Glu Glu His 
            420                 425                 430 

Gly Ser Asp Arg Trp Phe Asn Ala Leu Gly Ser Arg Arg Phe Ser Arg 
        435                 440                 445 

Asn Ala Cys Val Val Val Ser Cys Gly Thr Ala Val Thr Val Asp Ala 
    450                 455                 460 

Leu Thr Asp Asp Gly His Tyr Leu Gly Gly Thr Ile Met Pro Gly Phe 
465                 470                 475                 480 

His Leu Met Lys Glu Ser Leu Ala Val Arg Thr Ala Asn Leu Asn Arg 
                485                 490                 495 

His Ala Gly Lys Arg Tyr Pro Phe Pro Thr Thr Thr Gly Asn Ala Val 
            500                 505                 510 

Ala Ser Gly Met Met Asp Ala Val Cys Gly Ser Val Met Met Met His 
        515                 520                 525 

Gly Arg Leu Lys Glu Lys Thr Gly Ala Gly Lys Pro Val Asp Val Ile 
    530                 535                 540 

Ile Thr Gly Gly Gly Ala Ala Lys Val Ala Glu Ala Leu Pro Pro Ala 
545                 550                 555                 560 

Phe Leu Ala Glu Asn Thr Val Arg Val Ala Asp Asn Leu Val Ile Tyr 
                565                 570                 575 

Gly Leu Leu Asn Met Ile Ala Ala Glu Gly Arg Glu Tyr Glu His Ile 
            580                 585                 590 

 
           
             49  
             864  
             DNA  
             Neisseria meningitidis  
             
               CDS  
               (1)..(864)  
               NMB2074  
             
           
            49 

atg aaa tgg cta ttt atc ctt ttg gtt gcg att aat att gcc gta ttc       48 
Met Lys Trp Leu Phe Ile Leu Leu Val Ala Ile Asn Ile Ala Val Phe 
1               5                   10                  15 

ggc ggt acg gta ggt tac aaa ctg aca ctg aaa cag gcc ggc aga ata       96 
Gly Gly Thr Val Gly Tyr Lys Leu Thr Leu Lys Gln Ala Gly Arg Ile 
            20                  25                  30 

ccg gag gca cag aat gcc gca aac aat ttg cag gtt caa cca gtt gcc      144 
Pro Glu Ala Gln Asn Ala Ala Asn Asn Leu Gln Val Gln Pro Val Ala 
        35                  40                  45 

cca act atg ccg gtt gtt cgg aat att cca gca tcc ggt cct gtc gtt      192 
Pro Thr Met Pro Val Val Arg Asn Ile Pro Ala Ser Gly Pro Val Val 
    50                  55                  60 

cag gcg gca tct gaa tcg gat aca ggc gca ctg ctc aaa cag ggc gac      240 
Gln Ala Ala Ser Glu Ser Asp Thr Gly Ala Leu Leu Lys Gln Gly Asp 
65                  70                  75                  80 

att ctg agc gaa gaa cag gcg gag cag ttg cgc ttg aaa aaa gaa gcg      288 
Ile Leu Ser Glu Glu Gln Ala Glu Gln Leu Arg Leu Lys Lys Glu Ala 
                85                  90                  95 

gaa cag aaa aaa ctg aaa gag aaa aaa cag cgt gaa gaa aaa gcc cgc      336 
Glu Gln Lys Lys Leu Lys Glu Lys Lys Gln Arg Glu Glu Lys Ala Arg 
            100                 105                 110 

cgc gaa aaa ctc gcc gcc gaa aag gcg cag gcg gaa cgc gaa aac ggc      384 
Arg Glu Lys Leu Ala Ala Glu Lys Ala Gln Ala Glu Arg Glu Asn Gly 
        115                 120                 125 

gcg gcg gat gcc tta tgc gcc gcg cag gca agc ctc acg atg gac gaa      432 
Ala Ala Asp Ala Leu Cys Ala Ala Gln Ala Ser Leu Thr Met Asp Glu 
    130                 135                 140 

gat gac tac cac cgc atc aaa gga ctt ttg ggc aaa tgg tcg cac gtt      480 
Asp Asp Tyr His Arg Ile Lys Gly Leu Leu Gly Lys Trp Ser His Val 
145                 150                 155                 160 

gcc agc agg agc gtc gaa aaa cgc acc gcc caa gcc aaa cct gcc gac      528 
Ala Ser Arg Ser Val Glu Lys Arg Thr Ala Gln Ala Lys Pro Ala Asp 
                165                 170                 175 

aaa acc tac cgc gtc gtc ctg ccc gtt tcc gcc gat gcc gaa aat cag      576 
Lys Thr Tyr Arg Val Val Leu Pro Val Ser Ala Asp Ala Glu Asn Gln 
            180                 185                 190 

gcg gcg gag ctg tct gcc aaa ggt ttc aac ccc ata ccg ttt gac ggc      624 
Ala Ala Glu Leu Ser Ala Lys Gly Phe Asn Pro Ile Pro Phe Asp Gly 
        195                 200                 205 

gca ttg agt ttg ggt gtc ggc aac agc cgg gaa aac gcc caa gcc ctg      672 
Ala Leu Ser Leu Gly Val Gly Asn Ser Arg Glu Asn Ala Gln Ala Leu 
    210                 215                 220 

caa aac cgg ctt gcc ggc gcc gga ttc ggc ggg gcg cat att gtc gaa      720 
Gln Asn Arg Leu Ala Gly Ala Gly Phe Gly Gly Ala His Ile Val Glu 
225                 230                 235                 240 

cac ttt gcc gaa gcc gac agg cag gac gat tct ttg agc gtg tcg cgt      768 
His Phe Ala Glu Ala Asp Arg Gln Asp Asp Ser Leu Ser Val Ser Arg 
                245                 250                 255 

atg acg gtt ttg ttt acc ggc gtg aat gcc gcc gat gcg gac gaa att      816 
Met Thr Val Leu Phe Thr Gly Val Asn Ala Ala Asp Ala Asp Glu Ile 
            260                 265                 270 

cgt aaa atc acg tcc cta tac ggc aaa ctg aac ctc aag tct tgc aaa      864 
Arg Lys Ile Thr Ser Leu Tyr Gly Lys Leu Asn Leu Lys Ser Cys Lys 
        275                 280                 285 

 
           
             50  
             288  
             PRT  
             Neisseria meningitidis  
           
            50 

Met Lys Trp Leu Phe Ile Leu Leu Val Ala Ile Asn Ile Ala Val Phe 
1               5                   10                  15 

Gly Gly Thr Val Gly Tyr Lys Leu Thr Leu Lys Gln Ala Gly Arg Ile 
            20                  25                  30 

Pro Glu Ala Gln Asn Ala Ala Asn Asn Leu Gln Val Gln Pro Val Ala 
        35                  40                  45 

Pro Thr Met Pro Val Val Arg Asn Ile Pro Ala Ser Gly Pro Val Val 
    50                  55                  60 

Gln Ala Ala Ser Glu Ser Asp Thr Gly Ala Leu Leu Lys Gln Gly Asp 
65                  70                  75                  80 

Ile Leu Ser Glu Glu Gln Ala Glu Gln Leu Arg Leu Lys Lys Glu Ala 
                85                  90                  95 

Glu Gln Lys Lys Leu Lys Glu Lys Lys Gln Arg Glu Glu Lys Ala Arg 
            100                 105                 110 

Arg Glu Lys Leu Ala Ala Glu Lys Ala Gln Ala Glu Arg Glu Asn Gly 
        115                 120                 125 

Ala Ala Asp Ala Leu Cys Ala Ala Gln Ala Ser Leu Thr Met Asp Glu 
    130                 135                 140 

Asp Asp Tyr His Arg Ile Lys Gly Leu Leu Gly Lys Trp Ser His Val 
145                 150                 155                 160 

Ala Ser Arg Ser Val Glu Lys Arg Thr Ala Gln Ala Lys Pro Ala Asp 
                165                 170                 175 

Lys Thr Tyr Arg Val Val Leu Pro Val Ser Ala Asp Ala Glu Asn Gln 
            180                 185                 190 

Ala Ala Glu Leu Ser Ala Lys Gly Phe Asn Pro Ile Pro Phe Asp Gly 
        195                 200                 205 

Ala Leu Ser Leu Gly Val Gly Asn Ser Arg Glu Asn Ala Gln Ala Leu 
    210                 215                 220 

Gln Asn Arg Leu Ala Gly Ala Gly Phe Gly Gly Ala His Ile Val Glu 
225                 230                 235                 240 

His Phe Ala Glu Ala Asp Arg Gln Asp Asp Ser Leu Ser Val Ser Arg 
                245                 250                 255 

Met Thr Val Leu Phe Thr Gly Val Asn Ala Ala Asp Ala Asp Glu Ile 
            260                 265                 270 

Arg Lys Ile Thr Ser Leu Tyr Gly Lys Leu Asn Leu Lys Ser Cys Lys 
        275                 280                 285 

 
           
             51  
             786  
             DNA  
             Neisseria meningitidis  
             
               CDS  
               (1)..(786)  
               NMA 0385  
             
           
            51 

atg aaa caa gct atg aaa aat tgg ttt gct gcc ttg ctg ttg gca gta       48 
Met Lys Gln Ala Met Lys Asn Trp Phe Ala Ala Leu Leu Leu Ala Val 
1               5                   10                  15 

ccc atg ggt gcg gca ttt gct tcg ggc ggt cat gca cac tat gag aaa       96 
Pro Met Gly Ala Ala Phe Ala Ser Gly Gly His Ala His Tyr Glu Lys 
            20                  25                  30 

gtc gat atc gat ttg cgc gac caa gtc agc ctg cag cgc ggc gca caa      144 
Val Asp Ile Asp Leu Arg Asp Gln Val Ser Leu Gln Arg Gly Ala Gln 
        35                  40                  45 

atc ttt acc aac tat tgc ctg tcc tgc cac tcg gca agc ggt atg cgt      192 
Ile Phe Thr Asn Tyr Cys Leu Ser Cys His Ser Ala Ser Gly Met Arg 
    50                  55                  60 

ttc aac cgt ttg aaa gac atc ggt ttg act gac gaa gaa atc aag aaa      240 
Phe Asn Arg Leu Lys Asp Ile Gly Leu Thr Asp Glu Glu Ile Lys Lys 
65                  70                  75                  80 

aac ctg atg ttt acc acc gat aat gtc ggc gat gtc atg cat tcg gcg      288 
Asn Leu Met Phe Thr Thr Asp Asn Val Gly Asp Val Met His Ser Ala 
                85                  90                  95 

atg aac ccg aaa gat gcg gca aaa tgg ttt ggt gct gct ccg ccc gat      336 
Met Asn Pro Lys Asp Ala Ala Lys Trp Phe Gly Ala Ala Pro Pro Asp 
            100                 105                 110 

ttg aca ttg att gcg cgt tcc aaa ggt gca gac tac ctt tac gct tat      384 
Leu Thr Leu Ile Ala Arg Ser Lys Gly Ala Asp Tyr Leu Tyr Ala Tyr 
        115                 120                 125 

atg cgc ggc ttc tat aaa gat ccg acc cgt ccg agc ggc tgg aac aat      432 
Met Arg Gly Phe Tyr Lys Asp Pro Thr Arg Pro Ser Gly Trp Asn Asn 
    130                 135                 140 

act gta ttc gat aaa gtc ggt atg ccc cac ccg ttg tgg gaa cag caa      480 
Thr Val Phe Asp Lys Val Gly Met Pro His Pro Leu Trp Glu Gln Gln 
145                 150                 155                 160 

ggc gtt caa gcc gtt gag ttg gat gcc aaa ggt cag ccg gtt atg gta      528 
Gly Val Gln Ala Val Glu Leu Asp Ala Lys Gly Gln Pro Val Met Val 
                165                 170                 175 

aaa gac gaa cac ggc gag atg aag cct aag ctg tat tgg gaa tct acc      576 
Lys Asp Glu His Gly Glu Met Lys Pro Lys Leu Tyr Trp Glu Ser Thr 
            180                 185                 190 

ggt ttg cac agc cgc cgt ctg cct aac ggc aaa gtg atc caa aaa gag      624 
Gly Leu His Ser Arg Arg Leu Pro Asn Gly Lys Val Ile Gln Lys Glu 
        195                 200                 205 

tac gac gca tat gta cgc gat ttg gtc aat tac ctt gtg tac atg ggc      672 
Tyr Asp Ala Tyr Val Arg Asp Leu Val Asn Tyr Leu Val Tyr Met Gly 
    210                 215                 220 

gaa cct gca caa ctg caa cgc aaa cgt ata ggc tat gtc gtg atg att      720 
Glu Pro Ala Gln Leu Gln Arg Lys Arg Ile Gly Tyr Val Val Met Ile 
225                 230                 235                 240 

ttc cta ttt gcg gtt atg ctg cct ttg gcg tat ttc ctg aaa aaa gaa      768 
Phe Leu Phe Ala Val Met Leu Pro Leu Ala Tyr Phe Leu Lys Lys Glu 
                245                 250                 255 

tat tgg aaa gac gta cac                                              786 
Tyr Trp Lys Asp Val His 
            260 

 
           
             52  
             262  
             PRT  
             Neisseria meningitidis  
           
            52 

Met Lys Gln Ala Met Lys Asn Trp Phe Ala Ala Leu Leu Leu Ala Val 
1               5                   10                  15 

Pro Met Gly Ala Ala Phe Ala Ser Gly Gly His Ala His Tyr Glu Lys 
            20                  25                  30 

Val Asp Ile Asp Leu Arg Asp Gln Val Ser Leu Gln Arg Gly Ala Gln 
        35                  40                  45 

Ile Phe Thr Asn Tyr Cys Leu Ser Cys His Ser Ala Ser Gly Met Arg 
    50                  55                  60 

Phe Asn Arg Leu Lys Asp Ile Gly Leu Thr Asp Glu Glu Ile Lys Lys 
65                  70                  75                  80 

Asn Leu Met Phe Thr Thr Asp Asn Val Gly Asp Val Met His Ser Ala 
                85                  90                  95 

Met Asn Pro Lys Asp Ala Ala Lys Trp Phe Gly Ala Ala Pro Pro Asp 
            100                 105                 110 

Leu Thr Leu Ile Ala Arg Ser Lys Gly Ala Asp Tyr Leu Tyr Ala Tyr 
        115                 120                 125 

Met Arg Gly Phe Tyr Lys Asp Pro Thr Arg Pro Ser Gly Trp Asn Asn 
    130                 135                 140 

Thr Val Phe Asp Lys Val Gly Met Pro His Pro Leu Trp Glu Gln Gln 
145                 150                 155                 160 

Gly Val Gln Ala Val Glu Leu Asp Ala Lys Gly Gln Pro Val Met Val 
                165                 170                 175 

Lys Asp Glu His Gly Glu Met Lys Pro Lys Leu Tyr Trp Glu Ser Thr 
            180                 185                 190 

Gly Leu His Ser Arg Arg Leu Pro Asn Gly Lys Val Ile Gln Lys Glu 
        195                 200                 205 

Tyr Asp Ala Tyr Val Arg Asp Leu Val Asn Tyr Leu Val Tyr Met Gly 
    210                 215                 220 

Glu Pro Ala Gln Leu Gln Arg Lys Arg Ile Gly Tyr Val Val Met Ile 
225                 230                 235                 240 

Phe Leu Phe Ala Val Met Leu Pro Leu Ala Tyr Phe Leu Lys Lys Glu 
                245                 250                 255 

Tyr Trp Lys Asp Val His 
            260 

 
           
             53  
             1782  
             DNA  
             Neisseria meningitidis  
             
               CDS  
               (1)..(1782)  
               NMB2050  
             
           
            53 

atg gaa ctg atg act gtt ttg ctg cct ttg gcg gcg ttg gtg tcg ggc       48 
Met Glu Leu Met Thr Val Leu Leu Pro Leu Ala Ala Leu Val Ser Gly 
1               5                   10                  15 

gtg ttg ttt aca tgg ttg ctg atg aag ggc cgg ttt cag ggc gag ttt       96 
Val Leu Phe Thr Trp Leu Leu Met Lys Gly Arg Phe Gln Gly Glu Phe 
            20                  25                  30 

gcc ggt ttg aac gcg cac ctg gcg gaa aag gcg gca aga tgt gat ttt      144 
Ala Gly Leu Asn Ala His Leu Ala Glu Lys Ala Ala Arg Cys Asp Phe 
        35                  40                  45 

gtc gaa cag gca cac ggc aaa acc gtg tcg gaa ttg gcg gtg ttg gac      192 
Val Glu Gln Ala His Gly Lys Thr Val Ser Glu Leu Ala Val Leu Asp 
    50                  55                  60 

ggg aaa tac cgg cat ttg cag gac gaa aat tat gct ttg ggc aac cgt      240 
Gly Lys Tyr Arg His Leu Gln Asp Glu Asn Tyr Ala Leu Gly Asn Arg 
65                  70                  75                  80 

ttt tcc gca gcc gaa aag cag att gcc cat ttg cag gaa aaa gag gcg      288 
Phe Ser Ala Ala Glu Lys Gln Ile Ala His Leu Gln Glu Lys Glu Ala 
                85                  90                  95 

gag tcg gcg cgg ctg aag cag tcg tat atc gag ttg cag gaa aag gca      336 
Glu Ser Ala Arg Leu Lys Gln Ser Tyr Ile Glu Leu Gln Glu Lys Ala 
            100                 105                 110 

cag ggt ttg gcg gtt gaa aac gaa cgt ttg gca acg cag ctc gga cag      384 
Gln Gly Leu Ala Val Glu Asn Glu Arg Leu Ala Thr Gln Leu Gly Gln 
        115                 120                 125 

gaa cgg aag gcg ttt gcc gac caa tat gcc ttg gaa cgc caa atc cgc      432 
Glu Arg Lys Ala Phe Ala Asp Gln Tyr Ala Leu Glu Arg Gln Ile Arg 
    130                 135                 140 

caa aga atc gaa acc gat ttg gaa gaa agc cgc caa act gtc cgc gac      480 
Gln Arg Ile Glu Thr Asp Leu Glu Glu Ser Arg Gln Thr Val Arg Asp 
145                 150                 155                 160 

gtg caa aac gac ctt tcc gat gtc ggc aac cgt ttt gcc gca gcc gaa      528 
Val Gln Asn Asp Leu Ser Asp Val Gly Asn Arg Phe Ala Ala Ala Glu 
                165                 170                 175 

aaa cag att gcc cat ttg cag gaa aaa gag gcg gaa gcg gag cgg ttg      576 
Lys Gln Ile Ala His Leu Gln Glu Lys Glu Ala Glu Ala Glu Arg Leu 
            180                 185                 190 

agg cag tcg cat acc gag ttg cag gaa aag gca cag ggt ttg gcg gtt      624 
Arg Gln Ser His Thr Glu Leu Gln Glu Lys Ala Gln Gly Leu Ala Val 
        195                 200                 205 

gaa aac gaa cgt ttg gca acg caa atc gaa cag gaa cgc ctt gct tct      672 
Glu Asn Glu Arg Leu Ala Thr Gln Ile Glu Gln Glu Arg Leu Ala Ser 
    210                 215                 220 

gaa gag aag ctg tcc ttg ctg ggc gag gcg cgc aaa agt ttg agc gat      720 
Glu Glu Lys Leu Ser Leu Leu Gly Glu Ala Arg Lys Ser Leu Ser Asp 
225                 230                 235                 240 

cag ttt caa aat ctt gcc aac acg att ttg gaa gaa aaa agc cgc cgt      768 
Gln Phe Gln Asn Leu Ala Asn Thr Ile Leu Glu Glu Lys Ser Arg Arg 
                245                 250                 255 

ttt acc gag cag aac cgc gag cag ctc cat cag gtt ttg aac ccg cta      816 
Phe Thr Glu Gln Asn Arg Glu Gln Leu His Gln Val Leu Asn Pro Leu 
            260                 265                 270 

aac gaa cgc atc cac ggt ttc ggc gag ttg gtc aag caa acc tat gat      864 
Asn Glu Arg Ile His Gly Phe Gly Glu Leu Val Lys Gln Thr Tyr Asp 
        275                 280                 285 

aaa gaa tcg cgc gag cgg ctg acg ttg gaa aac gaa ttg aaa cgg ctt      912 
Lys Glu Ser Arg Glu Arg Leu Thr Leu Glu Asn Glu Leu Lys Arg Leu 
    290                 295                 300 

cag ggg ttg aac gcg cag ctg cac agc gag gca aag gcc ctg acc aac      960 
Gln Gly Leu Asn Ala Gln Leu His Ser Glu Ala Lys Ala Leu Thr Asn 
305                 310                 315                 320 

gcg ctg acc ggt acg cag aat aag gtt cag ggc aat tgg ggc gag atg     1008 
Ala Leu Thr Gly Thr Gln Asn Lys Val Gln Gly Asn Trp Gly Glu Met 
                325                 330                 335 

att ctg gaa acg gtt ttg gaa aat tcc ggc ctt cag aaa ggg cgg gaa     1056 
Ile Leu Glu Thr Val Leu Glu Asn Ser Gly Leu Gln Lys Gly Arg Glu 
            340                 345                 350 

tat gtg gtt cag gcg gca tcc gtc cga aaa gag gaa gac ggc ggc acg     1104 
Tyr Val Val Gln Ala Ala Ser Val Arg Lys Glu Glu Asp Gly Gly Thr 
        355                 360                 365 

cgc cgc ctc cag ccc gac gtt ttg gtc aac ctg ccc gac aac aag cag     1152 
Arg Arg Leu Gln Pro Asp Val Leu Val Asn Leu Pro Asp Asn Lys Gln 
    370                 375                 380 

att gtg att gat tcc aag gtc tcg ctg aca gct tat gtg cgc tac acg     1200 
Ile Val Ile Asp Ser Lys Val Ser Leu Thr Ala Tyr Val Arg Tyr Thr 
385                 390                 395                 400 

cag gcg gcg gat gcg gat acg gcg gca cgc gaa ctg gcg gca cac gtt     1248 
Gln Ala Ala Asp Ala Asp Thr Ala Ala Arg Glu Leu Ala Ala His Val 
                405                 410                 415 

gcc agc atc cgt gca cac atg aaa ggc ttg tcg ctg aag gat tac acc     1296 
Ala Ser Ile Arg Ala His Met Lys Gly Leu Ser Leu Lys Asp Tyr Thr 
            420                 425                 430 

gat ttg gaa ggt gtg aac aca ttg gat ttc gtc ttt atg ttt atc cct     1344 
Asp Leu Glu Gly Val Asn Thr Leu Asp Phe Val Phe Met Phe Ile Pro 
        435                 440                 445 

gtc gaa ccg gcc tac ctg ttg gcg ttg cag aat gac gcg ggc ttg ttc     1392 
Val Glu Pro Ala Tyr Leu Leu Ala Leu Gln Asn Asp Ala Gly Leu Phe 
    450                 455                 460 

caa gag tgt ttc gac aaa cgg att atg ctg gtc ggc ccc agt acg ctg     1440 
Gln Glu Cys Phe Asp Lys Arg Ile Met Leu Val Gly Pro Ser Thr Leu 
465                 470                 475                 480 

ctg gcg act ttg agg acg gtg gcg aat att tgg cgc aac gaa cag caa     1488 
Leu Ala Thr Leu Arg Thr Val Ala Asn Ile Trp Arg Asn Glu Gln Gln 
                485                 490                 495 

aat cag aac gca ctg gcg att gcg gac gaa ggc ggc aag ctg tac gac     1536 
Asn Gln Asn Ala Leu Ala Ile Ala Asp Glu Gly Gly Lys Leu Tyr Asp 
            500                 505                 510 

aag ttt gtc ggc ttc gta cag acg ctc gaa agc gtc ggc aaa ggc atc     1584 
Lys Phe Val Gly Phe Val Gln Thr Leu Glu Ser Val Gly Lys Gly Ile 
        515                 520                 525 

gat cag gcg caa agc agt ttt cag acg gca ttc aag caa ctt gcc gaa     1632 
Asp Gln Ala Gln Ser Ser Phe Gln Thr Ala Phe Lys Gln Leu Ala Glu 
    530                 535                 540 

ggg cgc ggg aat ctg gtc gga cgc gcc gag aaa ctg cgt ctg ttg ggc     1680 
Gly Arg Gly Asn Leu Val Gly Arg Ala Glu Lys Leu Arg Leu Leu Gly 
545                 550                 555                 560 

gtg aag gca ggc aaa caa ctt caa cgg gat ttg gtc gag cgt tcc aat     1728 
Val Lys Ala Gly Lys Gln Leu Gln Arg Asp Leu Val Glu Arg Ser Asn 
                565                 570                 575 

gaa aca acg gcg ttg tcg gaa tct ttg gaa tac gcg gca gaa gat gaa     1776 
Glu Thr Thr Ala Leu Ser Glu Ser Leu Glu Tyr Ala Ala Glu Asp Glu 
            580                 585                 590 

gca gtc                                                             1782 
Ala Val 

 
           
             54  
             594  
             PRT  
             Neisseria meningitidis  
           
            54 

Met Glu Leu Met Thr Val Leu Leu Pro Leu Ala Ala Leu Val Ser Gly 
1               5                   10                  15 

Val Leu Phe Thr Trp Leu Leu Met Lys Gly Arg Phe Gln Gly Glu Phe 
            20                  25                  30 

Ala Gly Leu Asn Ala His Leu Ala Glu Lys Ala Ala Arg Cys Asp Phe 
        35                  40                  45 

Val Glu Gln Ala His Gly Lys Thr Val Ser Glu Leu Ala Val Leu Asp 
    50                  55                  60 

Gly Lys Tyr Arg His Leu Gln Asp Glu Asn Tyr Ala Leu Gly Asn Arg 
65                  70                  75                  80 

Phe Ser Ala Ala Glu Lys Gln Ile Ala His Leu Gln Glu Lys Glu Ala 
                85                  90                  95 

Glu Ser Ala Arg Leu Lys Gln Ser Tyr Ile Glu Leu Gln Glu Lys Ala 
            100                 105                 110 

Gln Gly Leu Ala Val Glu Asn Glu Arg Leu Ala Thr Gln Leu Gly Gln 
        115                 120                 125 

Glu Arg Lys Ala Phe Ala Asp Gln Tyr Ala Leu Glu Arg Gln Ile Arg 
    130                 135                 140 

Gln Arg Ile Glu Thr Asp Leu Glu Glu Ser Arg Gln Thr Val Arg Asp 
145                 150                 155                 160 

Val Gln Asn Asp Leu Ser Asp Val Gly Asn Arg Phe Ala Ala Ala Glu 
                165                 170                 175 

Lys Gln Ile Ala His Leu Gln Glu Lys Glu Ala Glu Ala Glu Arg Leu 
            180                 185                 190 

Arg Gln Ser His Thr Glu Leu Gln Glu Lys Ala Gln Gly Leu Ala Val 
        195                 200                 205 

Glu Asn Glu Arg Leu Ala Thr Gln Ile Glu Gln Glu Arg Leu Ala Ser 
    210                 215                 220 

Glu Glu Lys Leu Ser Leu Leu Gly Glu Ala Arg Lys Ser Leu Ser Asp 
225                 230                 235                 240 

Gln Phe Gln Asn Leu Ala Asn Thr Ile Leu Glu Glu Lys Ser Arg Arg 
                245                 250                 255 

Phe Thr Glu Gln Asn Arg Glu Gln Leu His Gln Val Leu Asn Pro Leu 
            260                 265                 270 

Asn Glu Arg Ile His Gly Phe Gly Glu Leu Val Lys Gln Thr Tyr Asp 
        275                 280                 285 

Lys Glu Ser Arg Glu Arg Leu Thr Leu Glu Asn Glu Leu Lys Arg Leu 
    290                 295                 300 

Gln Gly Leu Asn Ala Gln Leu His Ser Glu Ala Lys Ala Leu Thr Asn 
305                 310                 315                 320 

Ala Leu Thr Gly Thr Gln Asn Lys Val Gln Gly Asn Trp Gly Glu Met 
                325                 330                 335 

Ile Leu Glu Thr Val Leu Glu Asn Ser Gly Leu Gln Lys Gly Arg Glu 
            340                 345                 350 

Tyr Val Val Gln Ala Ala Ser Val Arg Lys Glu Glu Asp Gly Gly Thr 
        355                 360                 365 

Arg Arg Leu Gln Pro Asp Val Leu Val Asn Leu Pro Asp Asn Lys Gln 
    370                 375                 380 

Ile Val Ile Asp Ser Lys Val Ser Leu Thr Ala Tyr Val Arg Tyr Thr 
385                 390                 395                 400 

Gln Ala Ala Asp Ala Asp Thr Ala Ala Arg Glu Leu Ala Ala His Val 
                405                 410                 415 

Ala Ser Ile Arg Ala His Met Lys Gly Leu Ser Leu Lys Asp Tyr Thr 
            420                 425                 430 

Asp Leu Glu Gly Val Asn Thr Leu Asp Phe Val Phe Met Phe Ile Pro 
        435                 440                 445 

Val Glu Pro Ala Tyr Leu Leu Ala Leu Gln Asn Asp Ala Gly Leu Phe 
    450                 455                 460 

Gln Glu Cys Phe Asp Lys Arg Ile Met Leu Val Gly Pro Ser Thr Leu 
465                 470                 475                 480 

Leu Ala Thr Leu Arg Thr Val Ala Asn Ile Trp Arg Asn Glu Gln Gln 
                485                 490                 495 

Asn Gln Asn Ala Leu Ala Ile Ala Asp Glu Gly Gly Lys Leu Tyr Asp 
            500                 505                 510 

Lys Phe Val Gly Phe Val Gln Thr Leu Glu Ser Val Gly Lys Gly Ile 
        515                 520                 525 

Asp Gln Ala Gln Ser Ser Phe Gln Thr Ala Phe Lys Gln Leu Ala Glu 
    530                 535                 540 

Gly Arg Gly Asn Leu Val Gly Arg Ala Glu Lys Leu Arg Leu Leu Gly 
545                 550                 555                 560 

Val Lys Ala Gly Lys Gln Leu Gln Arg Asp Leu Val Glu Arg Ser Asn 
                565                 570                 575 

Glu Thr Thr Ala Leu Ser Glu Ser Leu Glu Tyr Ala Ala Glu Asp Glu 
            580                 585                 590 

Ala Val 

 
           
             55  
             819  
             DNA  
             Neisseria meningitidis  
             
               CDS  
               (1)..(819)  
               HemK  
             
           
            55 

atg acg ttt gat aaa tgg ttg ggc ttg tca aaa ctg cct aaa aat gaa       48 
Met Thr Phe Asp Lys Trp Leu Gly Leu Ser Lys Leu Pro Lys Asn Glu 
1               5                   10                  15 

gca aga atg ctg ctg caa tat gtt tcg gaa tat acg cgc gtg cag ttg       96 
Ala Arg Met Leu Leu Gln Tyr Val Ser Glu Tyr Thr Arg Val Gln Leu 
            20                  25                  30 

ttg acg cgg ggc ggg gaa gaa atg ccg gac gaa gtc cga cag cgg gcg      144 
Leu Thr Arg Gly Gly Glu Glu Met Pro Asp Glu Val Arg Gln Arg Ala 
        35                  40                  45 

gac agg ctg gcg caa cgc cgt ctg aac ggc gag ccg gtt gcc tat att      192 
Asp Arg Leu Ala Gln Arg Arg Leu Asn Gly Glu Pro Val Ala Tyr Ile 
    50                  55                  60 

tta ggt gcg cgc gaa ttt tat ggc aga cgc ttt aca gtc aac ccg agc      240 
Leu Gly Ala Arg Glu Phe Tyr Gly Arg Arg Phe Thr Val Asn Pro Ser 
65                  70                  75                  80 

gtg ctg att ccg cgc ccc gaa acc gaa cat ttg gtc gaa gcc gta ttg      288 
Val Leu Ile Pro Arg Pro Glu Thr Glu His Leu Val Glu Ala Val Leu 
                85                  90                  95 

gcg cgc ctg ccg gaa aac ggg cgc gtg tgg gat ttg ggg acg ggc agc      336 
Ala Arg Leu Pro Glu Asn Gly Arg Val Trp Asp Leu Gly Thr Gly Ser 
            100                 105                 110 

gga gcg gtt gcc gta acc gtc gcg ctc gaa cgc ccc gat gcg ttt gta      384 
Gly Ala Val Ala Val Thr Val Ala Leu Glu Arg Pro Asp Ala Phe Val 
        115                 120                 125 

cgc gca tcc gac atc agc ccg ccc gcc ctt gaa acg gcg cgg aaa aat      432 
Arg Ala Ser Asp Ile Ser Pro Pro Ala Leu Glu Thr Ala Arg Lys Asn 
    130                 135                 140 

gcg gca gat ttg ggc gcg cgg gtc gaa ttt gca tac ggt tcg tgg ttc      480 
Ala Ala Asp Leu Gly Ala Arg Val Glu Phe Ala Tyr Gly Ser Trp Phe 
145                 150                 155                 160 

gac acc gat atg ccg tct gaa ggg aaa tgg gac atc atc gtg tcc aac      528 
Asp Thr Asp Met Pro Ser Glu Gly Lys Trp Asp Ile Ile Val Ser Asn 
                165                 170                 175 

ccg ccc tat atc gaa aac ggc gat aaa cat ttg tcg caa ggc gat ttg      576 
Pro Pro Tyr Ile Glu Asn Gly Asp Lys His Leu Ser Gln Gly Asp Leu 
            180                 185                 190 

cgg ttt gag ccg caa atc gcg ctg acc gac ttt tca gac ggc cta agc      624 
Arg Phe Glu Pro Gln Ile Ala Leu Thr Asp Phe Ser Asp Gly Leu Ser 
        195                 200                 205 

tgc atc cgc acc ttg gcg caa ggc gcg ccc gac cgt ttg gcg gag ggc      672 
Cys Ile Arg Thr Leu Ala Gln Gly Ala Pro Asp Arg Leu Ala Glu Gly 
    210                 215                 220 

ggt ttt tta ttg ctg gaa cac ggt ttc gat cag ggc gcg gcg gtg cgc      720 
Gly Phe Leu Leu Leu Glu His Gly Phe Asp Gln Gly Ala Ala Val Arg 
225                 230                 235                 240 

ggc gtg ttg gcg gag aat ggt ttt tca gga gtg gaa acc ctg ccg gat      768 
Gly Val Leu Ala Glu Asn Gly Phe Ser Gly Val Glu Thr Leu Pro Asp 
                245                 250                 255 

ttg gcg ggt ttg gac agg gtt acg ctg ggg aag tat atg aag cat ttg      816 
Leu Ala Gly Leu Asp Arg Val Thr Leu Gly Lys Tyr Met Lys His Leu 
            260                 265                 270 

aaa                                                                  819 
Lys 

 
           
             56  
             273  
             PRT  
             Neisseria meningitidis  
           
            56 

Met Thr Phe Asp Lys Trp Leu Gly Leu Ser Lys Leu Pro Lys Asn Glu 
1               5                   10                  15 

Ala Arg Met Leu Leu Gln Tyr Val Ser Glu Tyr Thr Arg Val Gln Leu 
            20                  25                  30 

Leu Thr Arg Gly Gly Glu Glu Met Pro Asp Glu Val Arg Gln Arg Ala 
        35                  40                  45 

Asp Arg Leu Ala Gln Arg Arg Leu Asn Gly Glu Pro Val Ala Tyr Ile 
    50                  55                  60 

Leu Gly Ala Arg Glu Phe Tyr Gly Arg Arg Phe Thr Val Asn Pro Ser 
65                  70                  75                  80 

Val Leu Ile Pro Arg Pro Glu Thr Glu His Leu Val Glu Ala Val Leu 
                85                  90                  95 

Ala Arg Leu Pro Glu Asn Gly Arg Val Trp Asp Leu Gly Thr Gly Ser 
            100                 105                 110 

Gly Ala Val Ala Val Thr Val Ala Leu Glu Arg Pro Asp Ala Phe Val 
        115                 120                 125 

Arg Ala Ser Asp Ile Ser Pro Pro Ala Leu Glu Thr Ala Arg Lys Asn 
    130                 135                 140 

Ala Ala Asp Leu Gly Ala Arg Val Glu Phe Ala Tyr Gly Ser Trp Phe 
145                 150                 155                 160 

Asp Thr Asp Met Pro Ser Glu Gly Lys Trp Asp Ile Ile Val Ser Asn 
                165                 170                 175 

Pro Pro Tyr Ile Glu Asn Gly Asp Lys His Leu Ser Gln Gly Asp Leu 
            180                 185                 190 

Arg Phe Glu Pro Gln Ile Ala Leu Thr Asp Phe Ser Asp Gly Leu Ser 
        195                 200                 205 

Cys Ile Arg Thr Leu Ala Gln Gly Ala Pro Asp Arg Leu Ala Glu Gly 
    210                 215                 220 

Gly Phe Leu Leu Leu Glu His Gly Phe Asp Gln Gly Ala Ala Val Arg 
225                 230                 235                 240 

Gly Val Leu Ala Glu Asn Gly Phe Ser Gly Val Glu Thr Leu Pro Asp 
                245                 250                 255 

Leu Ala Gly Leu Asp Arg Val Thr Leu Gly Lys Tyr Met Lys His Leu 
            260                 265                 270 

Lys 

 
           
             57  
             2148  
             DNA  
             Neisseria meningitidis  
             
               CDS  
               (1)..(2148)  
               NMB0287  
             
           
            57 

atg ctc acc gat tta gaa aaa aac gcc atc cgc gac cat tac caa aac       48 
Met Leu Thr Asp Leu Glu Lys Asn Ala Ile Arg Asp His Tyr Gln Asn 
1               5                   10                  15 

atc ggc aaa aac ctg ccc ggt ttc cgt ccg cgt gct tcg cag cgg gaa       96 
Ile Gly Lys Asn Leu Pro Gly Phe Arg Pro Arg Ala Ser Gln Arg Glu 
            20                  25                  30 

atg att gcg gcg gtt gcc aac gct ttt tcg cgg acg ttg gcg cgc gaa      144 
Met Ile Ala Ala Val Ala Asn Ala Phe Ser Arg Thr Leu Ala Arg Glu 
        35                  40                  45 

gaa ggc ggc gag ccg ccc aag cgc gaa ggc gag agc att gcc gtg atc      192 
Glu Gly Gly Glu Pro Pro Lys Arg Glu Gly Glu Ser Ile Ala Val Ile 
    50                  55                  60 

gaa ggg ccg acc ggc gtg ggc aaa tcg ttg gcc tac ctt ttg gcc ggc      240 
Glu Gly Pro Thr Gly Val Gly Lys Ser Leu Ala Tyr Leu Leu Ala Gly 
65                  70                  75                  80 

ggc atc atg gcg caa aca cgc ggc aag cgg ctg att gtg agc agc gcg      288 
Gly Ile Met Ala Gln Thr Arg Gly Lys Arg Leu Ile Val Ser Ser Ala 
                85                  90                  95 

acg gtt gcc ttg cag gag cag ttg gta gac cgc gac ctg ccg ttt ctg      336 
Thr Val Ala Leu Gln Glu Gln Leu Val Asp Arg Asp Leu Pro Phe Leu 
            100                 105                 110 

gtc gaa aaa agc ggt ttg gaa ctg acc ttc gca ctt gcc aaa ggg cgc      384 
Val Glu Lys Ser Gly Leu Glu Leu Thr Phe Ala Leu Ala Lys Gly Arg 
        115                 120                 125 

ggc cgc tat ctc tgc ccc tac aaa ctc tat cga ctg acg caa agc aat      432 
Gly Arg Tyr Leu Cys Pro Tyr Lys Leu Tyr Arg Leu Thr Gln Ser Asn 
    130                 135                 140 

gcc cag caa aac ctg ctc ggc ttt gaa gcc ccc gcc gtc ttg tgg gac      480 
Ala Gln Gln Asn Leu Leu Gly Phe Glu Ala Pro Ala Val Leu Trp Asp 
145                 150                 155                 160 

agc aaa ccc aag ccc gaa gaa ttg aag ctg ctg cgc gac atc gcc gac      528 
Ser Lys Pro Lys Pro Glu Glu Leu Lys Leu Leu Arg Asp Ile Ala Asp 
                165                 170                 175 

gaa ttt tcc gcc cga cgg ttc aac ggc gac cgc gac act tgg ccg gaa      576 
Glu Phe Ser Ala Arg Arg Phe Asn Gly Asp Arg Asp Thr Trp Pro Glu 
            180                 185                 190 

aaa atc gat gac gcg att tgg ctc aaa gtg acc aac gac cgc cac ggc      624 
Lys Ile Asp Asp Ala Ile Trp Leu Lys Val Thr Asn Asp Arg His Gly 
        195                 200                 205 

tgc ctg aaa acc gcc tgt ccc aac cgt ccg gaa tgt ccg ttt tac cta      672 
Cys Leu Lys Thr Ala Cys Pro Asn Arg Pro Glu Cys Pro Phe Tyr Leu 
    210                 215                 220 

gca cgc gat gtc ttg gaa acc gtc gat gtc gtc gtt gcc aac cac gat      720 
Ala Arg Asp Val Leu Glu Thr Val Asp Val Val Val Ala Asn His Asp 
225                 230                 235                 240 

ctt ctg ctt gcc gac atc agt atg ggc ggc ggc gtg att ctg cct gcg      768 
Leu Leu Leu Ala Asp Ile Ser Met Gly Gly Gly Val Ile Leu Pro Ala 
                245                 250                 255 

ccc gaa aac agt ttc tat tgc atc gac gaa gcg cac cac ctg ccc aaa      816 
Pro Glu Asn Ser Phe Tyr Cys Ile Asp Glu Ala His His Leu Pro Lys 
            260                 265                 270 

aaa gcc ctc agc cgt ttt gcc gcc gaa cat tca tgg aat att gcc gtt      864 
Lys Ala Leu Ser Arg Phe Ala Ala Glu His Ser Trp Asn Ile Ala Val 
        275                 280                 285 

tgg acg ctg gaa aaa ctg ccg cag ctg acc ggc aaa att gcc gcg ctg      912 
Trp Thr Leu Glu Lys Leu Pro Gln Leu Thr Gly Lys Ile Ala Ala Leu 
    290                 295                 300 

acc gat aaa gcc gaa ctt gcc aac cta gcc gac gaa gcc gcc gca tcc      960 
Thr Asp Lys Ala Glu Leu Ala Asn Leu Ala Asp Glu Ala Ala Ala Ser 
305                 310                 315                 320 

ttg ctc gac agc ctg cat gaa tgg caa ttc cat ttg gcg gaa gag ccg     1008 
Leu Leu Asp Ser Leu His Glu Trp Gln Phe His Leu Ala Glu Glu Pro 
                325                 330                 335 

tct tta agt ctg ggg gtg tct gaa aac gac aga cga acc aac agc gaa     1056 
Ser Leu Ser Leu Gly Val Ser Glu Asn Asp Arg Arg Thr Asn Ser Glu 
            340                 345                 350 

ccg act tgg ctg tgg gaa gac ggc aaa atc ccc gaa ggc ctc gaa acc     1104 
Pro Thr Trp Leu Trp Glu Asp Gly Lys Ile Pro Glu Gly Leu Glu Thr 
        355                 360                 365 

acc gtt tcc aat acg gcc att gct gcg cgc agc ctg ctc aaa cac gtt     1152 
Thr Val Ser Asn Thr Ala Ile Ala Ala Arg Ser Leu Leu Lys His Val 
    370                 375                 380 

atc ggg ctg aac gat gcg ctt tct gcc gca cgc cgc gaa aaa gaa cag     1200 
Ile Gly Leu Asn Asp Ala Leu Ser Ala Ala Arg Arg Glu Lys Glu Gln 
385                 390                 395                 400 

gac ggc gcg ctc ctc gac cgc ctg acc agc gag ttc ggt ctt ttt atc     1248 
Asp Gly Ala Leu Leu Asp Arg Leu Thr Ser Glu Phe Gly Leu Phe Ile 
                405                 410                 415 

gcc cgt atc gaa caa atc agc gcg gtt tgg gat ttg ctc tcc act gtc     1296 
Ala Arg Ile Glu Gln Ile Ser Ala Val Trp Asp Leu Leu Ser Thr Val 
            420                 425                 430 

ccc ctc gag ggt gaa gaa ccg ttg gcg aaa tgg ata acc cgc cgc gcc     1344 
Pro Leu Glu Gly Glu Glu Pro Leu Ala Lys Trp Ile Thr Arg Arg Ala 
        435                 440                 445 

gac gac aaa aac gac tac att ttc aac gcc agc ccc atc agc agc gca     1392 
Asp Asp Lys Asn Asp Tyr Ile Phe Asn Ala Ser Pro Ile Ser Ser Ala 
    450                 455                 460 

tcc cac ctt gcc aac agc ctg tgg cgg cgt gcg gca ggc gcg gta ttg     1440 
Ser His Leu Ala Asn Ser Leu Trp Arg Arg Ala Ala Gly Ala Val Leu 
465                 470                 475                 480 

act tcc gcc acc ctg caa tcc ttg ggc aac ttc aac ctg atg ctg cgc     1488 
Thr Ser Ala Thr Leu Gln Ser Leu Gly Asn Phe Asn Leu Met Leu Arg 
                485                 490                 495 

caa acc ggg ctg caa tgg ctg ccc gaa acc acc acc ctc gcc ctc aaa     1536 
Gln Thr Gly Leu Gln Trp Leu Pro Glu Thr Thr Thr Leu Ala Leu Lys 
            500                 505                 510 

agc ccc ttt gac ttt gaa aaa cag ggc gaa ctc tac atc ccc ccc ata     1584 
Ser Pro Phe Asp Phe Glu Lys Gln Gly Glu Leu Tyr Ile Pro Pro Ile 
        515                 520                 525 

tac gcc agc ccc aaa gac ccc gaa gcc cac acc gcc gcc gtc atc gaa     1632 
Tyr Ala Ser Pro Lys Asp Pro Glu Ala His Thr Ala Ala Val Ile Glu 
    530                 535                 540 

tgg ctg ccc aag ctt att tcg ccc acc gaa gcc atc ggc acg ctc gtc     1680 
Trp Leu Pro Lys Leu Ile Ser Pro Thr Glu Ala Ile Gly Thr Leu Val 
545                 550                 555                 560 

ttg ttt tcc tcg cgc aaa caa atg cag gat gtc gcc ctg cgc ctg ccc     1728 
Leu Phe Ser Ser Arg Lys Gln Met Gln Asp Val Ala Leu Arg Leu Pro 
                565                 570                 575 

gga gac tac ctg ccg ctc ttg ctc gta caa ggc gaa tta ccc aaa gcc     1776 
Gly Asp Tyr Leu Pro Leu Leu Leu Val Gln Gly Glu Leu Pro Lys Ala 
            580                 585                 590 

gtc ctc ctg caa aaa cac cac cgg gcc ata gaa gaa ggc aaa gcc agc     1824 
Val Leu Leu Gln Lys His His Arg Ala Ile Glu Glu Gly Lys Ala Ser 
        595                 600                 605 

atc atc ttc gga ctc gac agc ttt gcc gaa gga ctc gac ctg ccc ggc     1872 
Ile Ile Phe Gly Leu Asp Ser Phe Ala Glu Gly Leu Asp Leu Pro Gly 
    610                 615                 620 

acc gcc tgc gtg caa gtc atc atc gcc aaa ctt ccc ttc gcc atg ccc     1920 
Thr Ala Cys Val Gln Val Ile Ile Ala Lys Leu Pro Phe Ala Met Pro 
625                 630                 635                 640 

gac aac ccc atc gaa aaa acc caa aac cgc tgg ata gaa cag cgc ggc     1968 
Asp Asn Pro Ile Glu Lys Thr Gln Asn Arg Trp Ile Glu Gln Arg Gly 
                645                 650                 655 

ggc aac ccc ttc atc gaa atc acc gtc ccc gaa gcc ggc atc aaa ctc     2016 
Gly Asn Pro Phe Ile Glu Ile Thr Val Pro Glu Ala Gly Ile Lys Leu 
            660                 665                 670 

atc cag gcc gtc ggc cgc ctc atc cgc acc gaa caa gac tac ggc cgc     2064 
Ile Gln Ala Val Gly Arg Leu Ile Arg Thr Glu Gln Asp Tyr Gly Arg 
        675                 680                 685 

gta acc atc ctc gac aac cgc atc aaa aca cag cgg tac ggc caa caa     2112 
Val Thr Ile Leu Asp Asn Arg Ile Lys Thr Gln Arg Tyr Gly Gln Gln 
    690                 695                 700 

tta ttg gcc ggc ctg ccg ccg ttt aaa agg ata ggg                     2148 
Leu Leu Ala Gly Leu Pro Pro Phe Lys Arg Ile Gly 
705                 710                 715 

 
           
             58  
             716  
             PRT  
             Neisseria meningitidis  
           
            58 

Met Leu Thr Asp Leu Glu Lys Asn Ala Ile Arg Asp His Tyr Gln Asn 
1               5                   10                  15 

Ile Gly Lys Asn Leu Pro Gly Phe Arg Pro Arg Ala Ser Gln Arg Glu 
            20                  25                  30 

Met Ile Ala Ala Val Ala Asn Ala Phe Ser Arg Thr Leu Ala Arg Glu 
        35                  40                  45 

Glu Gly Gly Glu Pro Pro Lys Arg Glu Gly Glu Ser Ile Ala Val Ile 
    50                  55                  60 

Glu Gly Pro Thr Gly Val Gly Lys Ser Leu Ala Tyr Leu Leu Ala Gly 
65                  70                  75                  80 

Gly Ile Met Ala Gln Thr Arg Gly Lys Arg Leu Ile Val Ser Ser Ala 
                85                  90                  95 

Thr Val Ala Leu Gln Glu Gln Leu Val Asp Arg Asp Leu Pro Phe Leu 
            100                 105                 110 

Val Glu Lys Ser Gly Leu Glu Leu Thr Phe Ala Leu Ala Lys Gly Arg 
        115                 120                 125 

Gly Arg Tyr Leu Cys Pro Tyr Lys Leu Tyr Arg Leu Thr Gln Ser Asn 
    130                 135                 140 

Ala Gln Gln Asn Leu Leu Gly Phe Glu Ala Pro Ala Val Leu Trp Asp 
145                 150                 155                 160 

Ser Lys Pro Lys Pro Glu Glu Leu Lys Leu Leu Arg Asp Ile Ala Asp 
                165                 170                 175 

Glu Phe Ser Ala Arg Arg Phe Asn Gly Asp Arg Asp Thr Trp Pro Glu 
            180                 185                 190 

Lys Ile Asp Asp Ala Ile Trp Leu Lys Val Thr Asn Asp Arg His Gly 
        195                 200                 205 

Cys Leu Lys Thr Ala Cys Pro Asn Arg Pro Glu Cys Pro Phe Tyr Leu 
    210                 215                 220 

Ala Arg Asp Val Leu Glu Thr Val Asp Val Val Val Ala Asn His Asp 
225                 230                 235                 240 

Leu Leu Leu Ala Asp Ile Ser Met Gly Gly Gly Val Ile Leu Pro Ala 
                245                 250                 255 

Pro Glu Asn Ser Phe Tyr Cys Ile Asp Glu Ala His His Leu Pro Lys 
            260                 265                 270 

Lys Ala Leu Ser Arg Phe Ala Ala Glu His Ser Trp Asn Ile Ala Val 
        275                 280                 285 

Trp Thr Leu Glu Lys Leu Pro Gln Leu Thr Gly Lys Ile Ala Ala Leu 
    290                 295                 300 

Thr Asp Lys Ala Glu Leu Ala Asn Leu Ala Asp Glu Ala Ala Ala Ser 
305                 310                 315                 320 

Leu Leu Asp Ser Leu His Glu Trp Gln Phe His Leu Ala Glu Glu Pro 
                325                 330                 335 

Ser Leu Ser Leu Gly Val Ser Glu Asn Asp Arg Arg Thr Asn Ser Glu 
            340                 345                 350 

Pro Thr Trp Leu Trp Glu Asp Gly Lys Ile Pro Glu Gly Leu Glu Thr 
        355                 360                 365 

Thr Val Ser Asn Thr Ala Ile Ala Ala Arg Ser Leu Leu Lys His Val 
    370                 375                 380 

Ile Gly Leu Asn Asp Ala Leu Ser Ala Ala Arg Arg Glu Lys Glu Gln 
385                 390                 395                 400 

Asp Gly Ala Leu Leu Asp Arg Leu Thr Ser Glu Phe Gly Leu Phe Ile 
                405                 410                 415 

Ala Arg Ile Glu Gln Ile Ser Ala Val Trp Asp Leu Leu Ser Thr Val 
            420                 425                 430 

Pro Leu Glu Gly Glu Glu Pro Leu Ala Lys Trp Ile Thr Arg Arg Ala 
        435                 440                 445 

Asp Asp Lys Asn Asp Tyr Ile Phe Asn Ala Ser Pro Ile Ser Ser Ala 
    450                 455                 460 

Ser His Leu Ala Asn Ser Leu Trp Arg Arg Ala Ala Gly Ala Val Leu 
465                 470                 475                 480 

Thr Ser Ala Thr Leu Gln Ser Leu Gly Asn Phe Asn Leu Met Leu Arg 
                485                 490                 495 

Gln Thr Gly Leu Gln Trp Leu Pro Glu Thr Thr Thr Leu Ala Leu Lys 
            500                 505                 510 

Ser Pro Phe Asp Phe Glu Lys Gln Gly Glu Leu Tyr Ile Pro Pro Ile 
        515                 520                 525 

Tyr Ala Ser Pro Lys Asp Pro Glu Ala His Thr Ala Ala Val Ile Glu 
    530                 535                 540 

Trp Leu Pro Lys Leu Ile Ser Pro Thr Glu Ala Ile Gly Thr Leu Val 
545                 550                 555                 560 

Leu Phe Ser Ser Arg Lys Gln Met Gln Asp Val Ala Leu Arg Leu Pro 
                565                 570                 575 

Gly Asp Tyr Leu Pro Leu Leu Leu Val Gln Gly Glu Leu Pro Lys Ala 
            580                 585                 590 

Val Leu Leu Gln Lys His His Arg Ala Ile Glu Glu Gly Lys Ala Ser 
        595                 600                 605 

Ile Ile Phe Gly Leu Asp Ser Phe Ala Glu Gly Leu Asp Leu Pro Gly 
    610                 615                 620 

Thr Ala Cys Val Gln Val Ile Ile Ala Lys Leu Pro Phe Ala Met Pro 
625                 630                 635                 640 

Asp Asn Pro Ile Glu Lys Thr Gln Asn Arg Trp Ile Glu Gln Arg Gly 
                645                 650                 655 

Gly Asn Pro Phe Ile Glu Ile Thr Val Pro Glu Ala Gly Ile Lys Leu 
            660                 665                 670 

Ile Gln Ala Val Gly Arg Leu Ile Arg Thr Glu Gln Asp Tyr Gly Arg 
        675                 680                 685 

Val Thr Ile Leu Asp Asn Arg Ile Lys Thr Gln Arg Tyr Gly Gln Gln 
    690                 695                 700 

Leu Leu Ala Gly Leu Pro Pro Phe Lys Arg Ile Gly 
705                 710                 715 

 
           
             59  
             558  
             DNA  
             Neisseria meningitidis  
             
               CDS  
               (1)..(558)  
               sodC CDS  
             
           
            59 

atg aat atg aaa acc tta tta gca cta gcg gtt agt gca gta tgt tca       48 
Met Asn Met Lys Thr Leu Leu Ala Leu Ala Val Ser Ala Val Cys Ser 
1               5                   10                  15 

gtt ggt gtt gcg caa gca cac gag cat aat acg ata cct aaa ggt gct       96 
Val Gly Val Ala Gln Ala His Glu His Asn Thr Ile Pro Lys Gly Ala 
            20                  25                  30 

tct att gaa gtg aaa gtg caa caa ctt gat cca gta aac ggt aac aaa      144 
Ser Ile Glu Val Lys Val Gln Gln Leu Asp Pro Val Asn Gly Asn Lys 
        35                  40                  45 

gat gtg ggt aca gtg act att act gaa tct aac tat ggt ctt gtg ttt      192 
Asp Val Gly Thr Val Thr Ile Thr Glu Ser Asn Tyr Gly Leu Val Phe 
    50                  55                  60 

acc cct gat tta caa gga tta agc gaa ggc tta cat ggt ttc cac atc      240 
Thr Pro Asp Leu Gln Gly Leu Ser Glu Gly Leu His Gly Phe His Ile 
65                  70                  75                  80 

cat gaa aac cca agc tgt gag cca aaa gaa aaa gaa ggt aaa ttg aca      288 
His Glu Asn Pro Ser Cys Glu Pro Lys Glu Lys Glu Gly Lys Leu Thr 
                85                  90                  95 

gct ggt tta ggc gca ggc ggt cac tgg gat cct aaa ggt gca aaa caa      336 
Ala Gly Leu Gly Ala Gly Gly His Trp Asp Pro Lys Gly Ala Lys Gln 
            100                 105                 110 

cat ggt tac cca tgg caa gat gat gca cac tta ggt gat tta cct gca      384 
His Gly Tyr Pro Trp Gln Asp Asp Ala His Leu Gly Asp Leu Pro Ala 
        115                 120                 125 

tta act gta ttg cat gat ggc aca gca aca aat cct gtt tta gca cca      432 
Leu Thr Val Leu His Asp Gly Thr Ala Thr Asn Pro Val Leu Ala Pro 
    130                 135                 140 

cgt ctt aaa cat tta gat gat gtt cgc ggt cac tct att atg atc cac      480 
Arg Leu Lys His Leu Asp Asp Val Arg Gly His Ser Ile Met Ile His 
145                 150                 155                 160 

acg ggt ggt gat aat cac tcc gat cat cca gct cca ctt ggc ggt ggc      528 
Thr Gly Gly Asp Asn His Ser Asp His Pro Ala Pro Leu Gly Gly Gly 
                165                 170                 175 

ggc cca cgt atg gca tgt ggc gtg att aaa                              558 
Gly Pro Arg Met Ala Cys Gly Val Ile Lys 
            180                 185 

 
           
             60  
             186  
             PRT  
             Neisseria meningitidis  
           
            60 

Met Asn Met Lys Thr Leu Leu Ala Leu Ala Val Ser Ala Val Cys Ser 
1               5                   10                  15 

Val Gly Val Ala Gln Ala His Glu His Asn Thr Ile Pro Lys Gly Ala 
            20                  25                  30 

Ser Ile Glu Val Lys Val Gln Gln Leu Asp Pro Val Asn Gly Asn Lys 
        35                  40                  45 

Asp Val Gly Thr Val Thr Ile Thr Glu Ser Asn Tyr Gly Leu Val Phe 
    50                  55                  60 

Thr Pro Asp Leu Gln Gly Leu Ser Glu Gly Leu His Gly Phe His Ile 
65                  70                  75                  80 

His Glu Asn Pro Ser Cys Glu Pro Lys Glu Lys Glu Gly Lys Leu Thr 
                85                  90                  95 

Ala Gly Leu Gly Ala Gly Gly His Trp Asp Pro Lys Gly Ala Lys Gln 
            100                 105                 110 

His Gly Tyr Pro Trp Gln Asp Asp Ala His Leu Gly Asp Leu Pro Ala 
        115                 120                 125 

Leu Thr Val Leu His Asp Gly Thr Ala Thr Asn Pro Val Leu Ala Pro 
    130                 135                 140 

Arg Leu Lys His Leu Asp Asp Val Arg Gly His Ser Ile Met Ile His 
145                 150                 155                 160 

Thr Gly Gly Asp Asn His Ser Asp His Pro Ala Pro Leu Gly Gly Gly 
                165                 170                 175 

Gly Pro Arg Met Ala Cys Gly Val Ile Lys 
            180                 185 

 
           
             61  
             1290  
             DNA  
             Neisseria meningitidis  
             
               CDS  
               (1)..(1290)  
               NMB0315  
             
           
            61 

atg gct gtc ttc cca ctt tcg gca aaa cat cgg aaa tac gcg ctg cgt       48 
Met Ala Val Phe Pro Leu Ser Ala Lys His Arg Lys Tyr Ala Leu Arg 
1               5                   10                  15 

gcg ctt gcc gtt tcg att att ttg gtg tcg gcg gca tac att gct tcg       96 
Ala Leu Ala Val Ser Ile Ile Leu Val Ser Ala Ala Tyr Ile Ala Ser 
            20                  25                  30 

aca gag agg acg gag cgc gtc aga ccg cag cgc gtg gaa caa aat ctg      144 
Thr Glu Arg Thr Glu Arg Val Arg Pro Gln Arg Val Glu Gln Asn Leu 
        35                  40                  45 

ccg ccg ctg tct tgg ggc ggc agc ggc gtt cag acg gca tat tgg gtg      192 
Pro Pro Leu Ser Trp Gly Gly Ser Gly Val Gln Thr Ala Tyr Trp Val 
    50                  55                  60 

cag gag gcg gtg cag ccg ggc gac tcg ctg gcg gac gtg ctg gcg cgt      240 
Gln Glu Ala Val Gln Pro Gly Asp Ser Leu Ala Asp Val Leu Ala Arg 
65                  70                  75                  80 

tcg ggt atg gcg cgg gac gag att gcc cga atc acg gaa aaa tat ggc      288 
Ser Gly Met Ala Arg Asp Glu Ile Ala Arg Ile Thr Glu Lys Tyr Gly 
                85                  90                  95 

ggc gaa gcc gat ttg cgg cat ttg cgt gcc gac cag tcg gtt cat gtt      336 
Gly Glu Ala Asp Leu Arg His Leu Arg Ala Asp Gln Ser Val His Val 
            100                 105                 110 

ttg gtc ggc ggc gac ggc ggc gcg cgc gaa gtg cag ttt ttt acc gac      384 
Leu Val Gly Gly Asp Gly Gly Ala Arg Glu Val Gln Phe Phe Thr Asp 
        115                 120                 125 

gaa gac ggc gag cgc aat ctg gtc gct ttg gaa aag aaa ggc ggc ata      432 
Glu Asp Gly Glu Arg Asn Leu Val Ala Leu Glu Lys Lys Gly Gly Ile 
    130                 135                 140 

tgg cgg cgg tcg gct tct gag gcg gat atg aag gtt ttg ccg acg ctg      480 
Trp Arg Arg Ser Ala Ser Glu Ala Asp Met Lys Val Leu Pro Thr Leu 
145                 150                 155                 160 

cgt tcg gtc gtg gtc aaa acg tcg gcg cgc ggt tcg ctg gcg cgg gcg      528 
Arg Ser Val Val Val Lys Thr Ser Ala Arg Gly Ser Leu Ala Arg Ala 
                165                 170                 175 

gaa gtg ccc gtc gaa atc cgc gaa tcc tta agc ggg att ttc gcc ggc      576 
Glu Val Pro Val Glu Ile Arg Glu Ser Leu Ser Gly Ile Phe Ala Gly 
            180                 185                 190 

cgc ttc agc ctt gac ggt ttg aag gaa ggc gat gcc gtg cgc ctg atg      624 
Arg Phe Ser Leu Asp Gly Leu Lys Glu Gly Asp Ala Val Arg Leu Met 
        195                 200                 205 

tac gac agc ctg tat ttc cac ggg cag cag gtg gcg gcg ggc gat att      672 
Tyr Asp Ser Leu Tyr Phe His Gly Gln Gln Val Ala Ala Gly Asp Ile 
    210                 215                 220 

ttg gcg gct gaa gtc gtt aag ggc ggc aca agg cat cag gcg ttc tat      720 
Leu Ala Ala Glu Val Val Lys Gly Gly Thr Arg His Gln Ala Phe Tyr 
225                 230                 235                 240 

tac cgt tcg gac aag gaa ggc gga ggg ggc ggc aat tat tat gat gaa      768 
Tyr Arg Ser Asp Lys Glu Gly Gly Gly Gly Gly Asn Tyr Tyr Asp Glu 
                245                 250                 255 

gac ggc aag gtg ttg cag gaa aaa ggc ggc ttc aac atc gag ccg ctg      816 
Asp Gly Lys Val Leu Gln Glu Lys Gly Gly Phe Asn Ile Glu Pro Leu 
            260                 265                 270 

gtc tat acg cgc att tct tcg ccg ttc ggc tac cgt atg cac ccc atc      864 
Val Tyr Thr Arg Ile Ser Ser Pro Phe Gly Tyr Arg Met His Pro Ile 
        275                 280                 285 

ctg cac aca tgg cgg ctg cac acg ggc atc gat tat gcc gca ccg cag      912 
Leu His Thr Trp Arg Leu His Thr Gly Ile Asp Tyr Ala Ala Pro Gln 
    290                 295                 300 

gga acg ccg gtc agg gct tcc gcc gac ggc gtg att acc ttt aaa ggc      960 
Gly Thr Pro Val Arg Ala Ser Ala Asp Gly Val Ile Thr Phe Lys Gly 
305                 310                 315                 320 

cgg aag ggc gga tac ggc aac gcg gtg atg ata cgc cac gcc aac ggt     1008 
Arg Lys Gly Gly Tyr Gly Asn Ala Val Met Ile Arg His Ala Asn Gly 
                325                 330                 335 

gtg gaa acg ctg tac gcg cac ttg agc gcg ttt tcg cag gcg gaa ggc     1056 
Val Glu Thr Leu Tyr Ala His Leu Ser Ala Phe Ser Gln Ala Glu Gly 
            340                 345                 350 

aat gtg cgc ggc ggc gag gtc atc ggt ttt gtc ggt tcg acc ggg cgt     1104 
Asn Val Arg Gly Gly Glu Val Ile Gly Phe Val Gly Ser Thr Gly Arg 
        355                 360                 365 

tcg acc ggg ccg cac ctg cat tac gag gcg cgc atc aac ggg cag ccc     1152 
Ser Thr Gly Pro His Leu His Tyr Glu Ala Arg Ile Asn Gly Gln Pro 
    370                 375                 380 

gtc aat cct gtt tcg gtc gca ttg ccg aca ccg gaa ttg acg cag gcg     1200 
Val Asn Pro Val Ser Val Ala Leu Pro Thr Pro Glu Leu Thr Gln Ala 
385                 390                 395                 400 

gac aag gcg gcg ttt gcc gcg cag aaa cag aag gcg gac gcg ctg ctt     1248 
Asp Lys Ala Ala Phe Ala Ala Gln Lys Gln Lys Ala Asp Ala Leu Leu 
                405                 410                 415 

gcg cgc ttg cgc ggc ata ccg gtt acc gtg tcg caa tcg gat             1290 
Ala Arg Leu Arg Gly Ile Pro Val Thr Val Ser Gln Ser Asp 
            420                 425                 430 

 
           
             62  
             430  
             PRT  
             Neisseria meningitidis  
           
            62 

Met Ala Val Phe Pro Leu Ser Ala Lys His Arg Lys Tyr Ala Leu Arg 
1               5                   10                  15 

Ala Leu Ala Val Ser Ile Ile Leu Val Ser Ala Ala Tyr Ile Ala Ser 
            20                  25                  30 

Thr Glu Arg Thr Glu Arg Val Arg Pro Gln Arg Val Glu Gln Asn Leu 
        35                  40                  45 

Pro Pro Leu Ser Trp Gly Gly Ser Gly Val Gln Thr Ala Tyr Trp Val 
    50                  55                  60 

Gln Glu Ala Val Gln Pro Gly Asp Ser Leu Ala Asp Val Leu Ala Arg 
65                  70                  75                  80 

Ser Gly Met Ala Arg Asp Glu Ile Ala Arg Ile Thr Glu Lys Tyr Gly 
                85                  90                  95 

Gly Glu Ala Asp Leu Arg His Leu Arg Ala Asp Gln Ser Val His Val 
            100                 105                 110 

Leu Val Gly Gly Asp Gly Gly Ala Arg Glu Val Gln Phe Phe Thr Asp 
        115                 120                 125 

Glu Asp Gly Glu Arg Asn Leu Val Ala Leu Glu Lys Lys Gly Gly Ile 
    130                 135                 140 

Trp Arg Arg Ser Ala Ser Glu Ala Asp Met Lys Val Leu Pro Thr Leu 
145                 150                 155                 160 

Arg Ser Val Val Val Lys Thr Ser Ala Arg Gly Ser Leu Ala Arg Ala 
                165                 170                 175 

Glu Val Pro Val Glu Ile Arg Glu Ser Leu Ser Gly Ile Phe Ala Gly 
            180                 185                 190 

Arg Phe Ser Leu Asp Gly Leu Lys Glu Gly Asp Ala Val Arg Leu Met 
        195                 200                 205 

Tyr Asp Ser Leu Tyr Phe His Gly Gln Gln Val Ala Ala Gly Asp Ile 
    210                 215                 220 

Leu Ala Ala Glu Val Val Lys Gly Gly Thr Arg His Gln Ala Phe Tyr 
225                 230                 235                 240 

Tyr Arg Ser Asp Lys Glu Gly Gly Gly Gly Gly Asn Tyr Tyr Asp Glu 
                245                 250                 255 

Asp Gly Lys Val Leu Gln Glu Lys Gly Gly Phe Asn Ile Glu Pro Leu 
            260                 265                 270 

Val Tyr Thr Arg Ile Ser Ser Pro Phe Gly Tyr Arg Met His Pro Ile 
        275                 280                 285 

Leu His Thr Trp Arg Leu His Thr Gly Ile Asp Tyr Ala Ala Pro Gln 
    290                 295                 300 

Gly Thr Pro Val Arg Ala Ser Ala Asp Gly Val Ile Thr Phe Lys Gly 
305                 310                 315                 320 

Arg Lys Gly Gly Tyr Gly Asn Ala Val Met Ile Arg His Ala Asn Gly 
                325                 330                 335 

Val Glu Thr Leu Tyr Ala His Leu Ser Ala Phe Ser Gln Ala Glu Gly 
            340                 345                 350 

Asn Val Arg Gly Gly Glu Val Ile Gly Phe Val Gly Ser Thr Gly Arg 
        355                 360                 365 

Ser Thr Gly Pro His Leu His Tyr Glu Ala Arg Ile Asn Gly Gln Pro 
    370                 375                 380 

Val Asn Pro Val Ser Val Ala Leu Pro Thr Pro Glu Leu Thr Gln Ala 
385                 390                 395                 400 

Asp Lys Ala Ala Phe Ala Ala Gln Lys Gln Lys Ala Asp Ala Leu Leu 
                405                 410                 415 

Ala Arg Leu Arg Gly Ile Pro Val Thr Val Ser Gln Ser Asp 
            420                 425                 430 

 
           
             63  
             603  
             DNA  
             Neisseria meningitidis  
             
               CDS  
               (1)..(603)  
               NMB1119  
             
           
            63 

atg acc atc tat ttc aaa aac ggc ttt tac gac gac aca ttg ggc ggc       48 
Met Thr Ile Tyr Phe Lys Asn Gly Phe Tyr Asp Asp Thr Leu Gly Gly 
1               5                   10                  15 

atc ccc gaa ggc gcg gtt gcc gtc cgc gcc gaa gaa tac gcc gcc ctt       96 
Ile Pro Glu Gly Ala Val Ala Val Arg Ala Glu Glu Tyr Ala Ala Leu 
            20                  25                  30 

ttg gca gga cag gcg cag ggc ggg cag att gcc gca gat tcc gac ggc      144 
Leu Ala Gly Gln Ala Gln Gly Gly Gln Ile Ala Ala Asp Ser Asp Gly 
        35                  40                  45 

cgc ccc gtt tta acc ccg ccg cgc ccg tcc gat tac cac gaa tgg gac      192 
Arg Pro Val Leu Thr Pro Pro Arg Pro Ser Asp Tyr His Glu Trp Asp 
    50                  55                  60 

ggc aaa aaa tgg aaa atc agc aaa gcc gcc gcc gcc gcc cgt ttc gcc      240 
Gly Lys Lys Trp Lys Ile Ser Lys Ala Ala Ala Ala Ala Arg Phe Ala 
65                  70                  75                  80 

aaa caa aaa acc gcc ttg gca ttc cgc ctc gcg gaa aag gcg gac gaa      288 
Lys Gln Lys Thr Ala Leu Ala Phe Arg Leu Ala Glu Lys Ala Asp Glu 
                85                  90                  95 

ctc aaa aac agc ctc ttg gcg ggc tat ccc caa gtg gaa atc gac agc      336 
Leu Lys Asn Ser Leu Leu Ala Gly Tyr Pro Gln Val Glu Ile Asp Ser 
            100                 105                 110 

ttt tac agg cag gaa aaa gaa gcc ctc gcg cgg cag gcg gac aac aac      384 
Phe Tyr Arg Gln Glu Lys Glu Ala Leu Ala Arg Gln Ala Asp Asn Asn 
        115                 120                 125 

gcc ccg acc ccg atg ctg gcg caa atc gcc gcc gca agg ggc gtg gaa      432 
Ala Pro Thr Pro Met Leu Ala Gln Ile Ala Ala Ala Arg Gly Val Glu 
    130                 135                 140 

ttg gac gtt ttg att gaa aaa gtt atc gaa aaa tcc gcc cgc ctg gct      480 
Leu Asp Val Leu Ile Glu Lys Val Ile Glu Lys Ser Ala Arg Leu Ala 
145                 150                 155                 160 

gtt gcc gcc ggc gcg att atc gga aag cgt cag cag ctc gaa gac aaa      528 
Val Ala Ala Gly Ala Ile Ile Gly Lys Arg Gln Gln Leu Glu Asp Lys 
                165                 170                 175 

ttg aac acc atc gaa acc gcg ccc gga ttg gac gcg ctg gaa aag gaa      576 
Leu Asn Thr Ile Glu Thr Ala Pro Gly Leu Asp Ala Leu Glu Lys Glu 
            180                 185                 190 

atc gaa gaa tgg acg cta aac atc ggc                                  603 
Ile Glu Glu Trp Thr Leu Asn Ile Gly 
        195                 200 

 
           
             64  
             201  
             PRT  
             Neisseria meningitidis  
           
            64 

Met Thr Ile Tyr Phe Lys Asn Gly Phe Tyr Asp Asp Thr Leu Gly Gly 
1               5                   10                  15 

Ile Pro Glu Gly Ala Val Ala Val Arg Ala Glu Glu Tyr Ala Ala Leu 
            20                  25                  30 

Leu Ala Gly Gln Ala Gln Gly Gly Gln Ile Ala Ala Asp Ser Asp Gly 
        35                  40                  45 

Arg Pro Val Leu Thr Pro Pro Arg Pro Ser Asp Tyr His Glu Trp Asp 
    50                  55                  60 

Gly Lys Lys Trp Lys Ile Ser Lys Ala Ala Ala Ala Ala Arg Phe Ala 
65                  70                  75                  80 

Lys Gln Lys Thr Ala Leu Ala Phe Arg Leu Ala Glu Lys Ala Asp Glu 
                85                  90                  95 

Leu Lys Asn Ser Leu Leu Ala Gly Tyr Pro Gln Val Glu Ile Asp Ser 
            100                 105                 110 

Phe Tyr Arg Gln Glu Lys Glu Ala Leu Ala Arg Gln Ala Asp Asn Asn 
        115                 120                 125 

Ala Pro Thr Pro Met Leu Ala Gln Ile Ala Ala Ala Arg Gly Val Glu 
    130                 135                 140 

Leu Asp Val Leu Ile Glu Lys Val Ile Glu Lys Ser Ala Arg Leu Ala 
145                 150                 155                 160 

Val Ala Ala Gly Ala Ile Ile Gly Lys Arg Gln Gln Leu Glu Asp Lys 
                165                 170                 175 

Leu Asn Thr Ile Glu Thr Ala Pro Gly Leu Asp Ala Leu Glu Lys Glu 
            180                 185                 190 

Ile Glu Glu Trp Thr Leu Asn Ile Gly 
        195                 200 

 
           
             65  
             681  
             DNA  
             Neisseria meningitidis  
             
               CDS  
               (1)..(681)  
               NMB1119  
             
           
            65 

atg ccg tct gaa aaa tgt tca gac ggc att tct tat aac ctt ttg ttt       48 
Met Pro Ser Glu Lys Cys Ser Asp Gly Ile Ser Tyr Asn Leu Leu Phe 
1               5                   10                  15 

gca gca cat aac caa tca gtc ttt cct att ccg ttc cga aat gca tac       96 
Ala Ala His Asn Gln Ser Val Phe Pro Ile Pro Phe Arg Asn Ala Tyr 
            20                  25                  30 

cat gcg ccc gta ccc aat att tgc gaa caa gga aag aaa atg gca cgt      144 
His Ala Pro Val Pro Asn Ile Cys Glu Gln Gly Lys Lys Met Ala Arg 
        35                  40                  45 

tta acc gta cac acc ctc gaa acc gcc ccc gaa gcc gcc aaa gcg cgc      192 
Leu Thr Val His Thr Leu Glu Thr Ala Pro Glu Ala Ala Lys Ala Arg 
    50                  55                  60 

gtc gag gcg gta ctt caa aac aac ggc ttt atc ccc aac ctt atc ggc      240 
Val Glu Ala Val Leu Gln Asn Asn Gly Phe Ile Pro Asn Leu Ile Gly 
65                  70                  75                  80 

gta tta tca aac gcc ccc gaa gcc ttg gcg ttt tac caa gaa gtc ggc      288 
Val Leu Ser Asn Ala Pro Glu Ala Leu Ala Phe Tyr Gln Glu Val Gly 
                85                  90                  95 

aag ctc aac gcc gcc aac agc ctg acc gcc ggc gaa gtc gaa gtc atc      336 
Lys Leu Asn Ala Ala Asn Ser Leu Thr Ala Gly Glu Val Glu Val Ile 
            100                 105                 110 

cag atc atc gcc gcc cgt acc aac caa tgc ggt ttt tgc gtg gca ggg      384 
Gln Ile Ile Ala Ala Arg Thr Asn Gln Cys Gly Phe Cys Val Ala Gly 
        115                 120                 125 

cac acc aaa ctc gca acc ctg aaa aaa ctc ctg tcc gaa caa tcc gtc      432 
His Thr Lys Leu Ala Thr Leu Lys Lys Leu Leu Ser Glu Gln Ser Val 
    130                 135                 140 

aaa gcc gcg cgc gct ttg gca gca ggc gaa ttt gac gat gcc aaa ctc      480 
Lys Ala Ala Arg Ala Leu Ala Ala Gly Glu Phe Asp Asp Ala Lys Leu 
145                 150                 155                 160 

ggc gcg ctc gcc gcc ttc acc caa gcc gta atg gcg aaa aaa ggc gcg      528 
Gly Ala Leu Ala Ala Phe Thr Gln Ala Val Met Ala Lys Lys Gly Ala 
                165                 170                 175 

gta tcc gac gag gaa ctc aaa gca ttt ttc gat gcg ggc tac aac cag      576 
Val Ser Asp Glu Glu Leu Lys Ala Phe Phe Asp Ala Gly Tyr Asn Gln 
            180                 185                 190 

cag cag gca gtc gaa gtc gtg atg ggc gta gcc ttg gca acc ctg tgc      624 
Gln Gln Ala Val Glu Val Val Met Gly Val Ala Leu Ala Thr Leu Cys 
        195                 200                 205 

aac tac gtc aac aac ctc gga caa acc gaa atc aac ccc gaa ttg cag      672 
Asn Tyr Val Asn Asn Leu Gly Gln Thr Glu Ile Asn Pro Glu Leu Gln 
    210                 215                 220 

gct tac gcc                                                          681 
Ala Tyr Ala 
225 

 
           
             66  
             227  
             PRT  
             Neisseria meningitidis  
           
            66 

Met Pro Ser Glu Lys Cys Ser Asp Gly Ile Ser Tyr Asn Leu Leu Phe 
1               5                   10                  15 

Ala Ala His Asn Gln Ser Val Phe Pro Ile Pro Phe Arg Asn Ala Tyr 
            20                  25                  30 

His Ala Pro Val Pro Asn Ile Cys Glu Gln Gly Lys Lys Met Ala Arg 
        35                  40                  45 

Leu Thr Val His Thr Leu Glu Thr Ala Pro Glu Ala Ala Lys Ala Arg 
    50                  55                  60 

Val Glu Ala Val Leu Gln Asn Asn Gly Phe Ile Pro Asn Leu Ile Gly 
65                  70                  75                  80 

Val Leu Ser Asn Ala Pro Glu Ala Leu Ala Phe Tyr Gln Glu Val Gly 
                85                  90                  95 

Lys Leu Asn Ala Ala Asn Ser Leu Thr Ala Gly Glu Val Glu Val Ile 
            100                 105                 110 

Gln Ile Ile Ala Ala Arg Thr Asn Gln Cys Gly Phe Cys Val Ala Gly 
        115                 120                 125 

His Thr Lys Leu Ala Thr Leu Lys Lys Leu Leu Ser Glu Gln Ser Val 
    130                 135                 140 

Lys Ala Ala Arg Ala Leu Ala Ala Gly Glu Phe Asp Asp Ala Lys Leu 
145                 150                 155                 160 

Gly Ala Leu Ala Ala Phe Thr Gln Ala Val Met Ala Lys Lys Gly Ala 
                165                 170                 175 

Val Ser Asp Glu Glu Leu Lys Ala Phe Phe Asp Ala Gly Tyr Asn Gln 
            180                 185                 190 

Gln Gln Ala Val Glu Val Val Met Gly Val Ala Leu Ala Thr Leu Cys 
        195                 200                 205 

Asn Tyr Val Asn Asn Leu Gly Gln Thr Glu Ile Asn Pro Glu Leu Gln 
    210                 215                 220 

Ala Tyr Ala 
225 

 
           
             67  
             681  
             DNA  
             Neisseria meningitidis  
             
               CDS  
               (1)..(681)  
               NMB0995  macrophage infectivity 
      potentiator-related protein  
             
           
            67 

atg ccg tct gaa aaa tgt tca gac ggc att tct tat aac ctt ttg ttt       48 
Met Pro Ser Glu Lys Cys Ser Asp Gly Ile Ser Tyr Asn Leu Leu Phe 
1               5                   10                  15 

gca gca cat aac caa tca gtc ttt cct att ccg ttc cga aat gca tac       96 
Ala Ala His Asn Gln Ser Val Phe Pro Ile Pro Phe Arg Asn Ala Tyr 
            20                  25                  30 

cat gcg ccc gta ccc aat att tgc gaa caa gga aag aaa atg gca cgt      144 
His Ala Pro Val Pro Asn Ile Cys Glu Gln Gly Lys Lys Met Ala Arg 
        35                  40                  45 

tta acc gta cac acc ctc gaa acc gcc ccc gaa gcc gcc aaa gcg cgc      192 
Leu Thr Val His Thr Leu Glu Thr Ala Pro Glu Ala Ala Lys Ala Arg 
    50                  55                  60 

gtc gag gcg gta ctt caa aac aac ggc ttt atc ccc aac ctt atc ggc      240 
Val Glu Ala Val Leu Gln Asn Asn Gly Phe Ile Pro Asn Leu Ile Gly 
65                  70                  75                  80 

gta tta tca aac gcc ccc gaa gcc ttg gcg ttt tac caa gaa gtc ggc      288 
Val Leu Ser Asn Ala Pro Glu Ala Leu Ala Phe Tyr Gln Glu Val Gly 
                85                  90                  95 

aag ctc aac gcc gcc aac agc ctg acc gcc ggc gaa gtc gaa gtc atc      336 
Lys Leu Asn Ala Ala Asn Ser Leu Thr Ala Gly Glu Val Glu Val Ile 
            100                 105                 110 

cag atc atc gcc gcc cgt acc aac caa tgc ggt ttt tgc gtg gca ggg      384 
Gln Ile Ile Ala Ala Arg Thr Asn Gln Cys Gly Phe Cys Val Ala Gly 
        115                 120                 125 

cac acc aaa ctc gca acc ctg aaa aaa ctc ctg tcc gaa caa tcc gtc      432 
His Thr Lys Leu Ala Thr Leu Lys Lys Leu Leu Ser Glu Gln Ser Val 
    130                 135                 140 

aaa gcc gcg cgc gct ttg gca gca ggc gaa ttt gac gat gcc aaa ctc      480 
Lys Ala Ala Arg Ala Leu Ala Ala Gly Glu Phe Asp Asp Ala Lys Leu 
145                 150                 155                 160 

ggc gcg ctc gcc gcc ttc acc caa gcc gta atg gcg aaa aaa ggc gcg      528 
Gly Ala Leu Ala Ala Phe Thr Gln Ala Val Met Ala Lys Lys Gly Ala 
                165                 170                 175 

gta tcc gac gag gaa ctc aaa gca ttt ttc gat gcg ggc tac aac cag      576 
Val Ser Asp Glu Glu Leu Lys Ala Phe Phe Asp Ala Gly Tyr Asn Gln 
            180                 185                 190 

cag cag gca gtc gaa gtc gtg atg ggc gta gcc ttg gca acc ctg tgc      624 
Gln Gln Ala Val Glu Val Val Met Gly Val Ala Leu Ala Thr Leu Cys 
        195                 200                 205 

aac tac gtc aac aac ctc gga caa acc gaa atc aac ccc gaa ttg cag      672 
Asn Tyr Val Asn Asn Leu Gly Gln Thr Glu Ile Asn Pro Glu Leu Gln 
    210                 215                 220 

gct tac gcc                                                          681 
Ala Tyr Ala 
225 

 
           
             68  
             227  
             PRT  
             Neisseria meningitidis  
           
            68 

Met Pro Ser Glu Lys Cys Ser Asp Gly Ile Ser Tyr Asn Leu Leu Phe 
1               5                   10                  15 

Ala Ala His Asn Gln Ser Val Phe Pro Ile Pro Phe Arg Asn Ala Tyr 
            20                  25                  30 

His Ala Pro Val Pro Asn Ile Cys Glu Gln Gly Lys Lys Met Ala Arg 
        35                  40                  45 

Leu Thr Val His Thr Leu Glu Thr Ala Pro Glu Ala Ala Lys Ala Arg 
    50                  55                  60 

Val Glu Ala Val Leu Gln Asn Asn Gly Phe Ile Pro Asn Leu Ile Gly 
65                  70                  75                  80 

Val Leu Ser Asn Ala Pro Glu Ala Leu Ala Phe Tyr Gln Glu Val Gly 
                85                  90                  95 

Lys Leu Asn Ala Ala Asn Ser Leu Thr Ala Gly Glu Val Glu Val Ile 
            100                 105                 110 

Gln Ile Ile Ala Ala Arg Thr Asn Gln Cys Gly Phe Cys Val Ala Gly 
        115                 120                 125 

His Thr Lys Leu Ala Thr Leu Lys Lys Leu Leu Ser Glu Gln Ser Val 
    130                 135                 140 

Lys Ala Ala Arg Ala Leu Ala Ala Gly Glu Phe Asp Asp Ala Lys Leu 
145                 150                 155                 160 

Gly Ala Leu Ala Ala Phe Thr Gln Ala Val Met Ala Lys Lys Gly Ala 
                165                 170                 175 

Val Ser Asp Glu Glu Leu Lys Ala Phe Phe Asp Ala Gly Tyr Asn Gln 
            180                 185                 190 

Gln Gln Ala Val Glu Val Val Met Gly Val Ala Leu Ala Thr Leu Cys 
        195                 200                 205 

Asn Tyr Val Asn Asn Leu Gly Gln Thr Glu Ile Asn Pro Glu Leu Gln 
    210                 215                 220 

Ala Tyr Ala 
225 

 
           
             69  
             939  
             DNA  
             Neisseria meningitidis  
             
               CDS  
               (1)..(939)  
               NMB0652  maf A  
             
           
            69 

atg aaa acc ctg ctc ctc ctc atc ccc ctc gtc ctc aca gcc tgc ggc       48 
Met Lys Thr Leu Leu Leu Leu Ile Pro Leu Val Leu Thr Ala Cys Gly 
1               5                   10                  15 

aca ctg acc ggc ata ccc gcc cac ggc ggc ggc aaa cgc ttt gcc gtc       96 
Thr Leu Thr Gly Ile Pro Ala His Gly Gly Gly Lys Arg Phe Ala Val 
            20                  25                  30 

gaa caa gaa ctc gtc gcc gca tcg tcc cgc gcc gcc gtc aaa gaa atg      144 
Glu Gln Glu Leu Val Ala Ala Ser Ser Arg Ala Ala Val Lys Glu Met 
        35                  40                  45 

gat ttg tcc gcc cta aaa gga cgc aaa gcc gcc ctt tac gtc tcc gtt      192 
Asp Leu Ser Ala Leu Lys Gly Arg Lys Ala Ala Leu Tyr Val Ser Val 
    50                  55                  60 

atg ggc gac caa ggt tcg ggc aac ata agc ggc gga cgc tac tct atc      240 
Met Gly Asp Gln Gly Ser Gly Asn Ile Ser Gly Gly Arg Tyr Ser Ile 
65                  70                  75                  80 

gac gca ctg ata cgc ggc ggc tac cac aac aac ccc gaa agt gcc acc      288 
Asp Ala Leu Ile Arg Gly Gly Tyr His Asn Asn Pro Glu Ser Ala Thr 
                85                  90                  95 

caa tac agc tac ccc gcc tac gac act acc gcc acc acc aaa tcc gac      336 
Gln Tyr Ser Tyr Pro Ala Tyr Asp Thr Thr Ala Thr Thr Lys Ser Asp 
            100                 105                 110 

gcg ctc tcc agc gta acc act tcc aca tcg ctt ttg aac gcc ccc gcc      384 
Ala Leu Ser Ser Val Thr Thr Ser Thr Ser Leu Leu Asn Ala Pro Ala 
        115                 120                 125 

gcc gcc ctg acg aaa aac agc gga cgc aaa ggc gaa cgc tcc gcc gga      432 
Ala Ala Leu Thr Lys Asn Ser Gly Arg Lys Gly Glu Arg Ser Ala Gly 
    130                 135                 140 

ctg tcc gtc aac ggc acg ggc gac tac cgc aac gaa acc ctg ctc gcc      480 
Leu Ser Val Asn Gly Thr Gly Asp Tyr Arg Asn Glu Thr Leu Leu Ala 
145                 150                 155                 160 

aac ccc cgc gac gtt tcc ttc ctg acc aac ctc atc caa acc gtc ttc      528 
Asn Pro Arg Asp Val Ser Phe Leu Thr Asn Leu Ile Gln Thr Val Phe 
                165                 170                 175 

tac ctg cgc ggc atc gaa gtc gta ccg ccc gaa tac gcc gac acc gac      576 
Tyr Leu Arg Gly Ile Glu Val Val Pro Pro Glu Tyr Ala Asp Thr Asp 
            180                 185                 190 

gta ttc gta acc gtc gac gta ttc ggc acc gtc cgc agc cgt acc gaa      624 
Val Phe Val Thr Val Asp Val Phe Gly Thr Val Arg Ser Arg Thr Glu 
        195                 200                 205 

ctg cac ctc tac aac gcc gaa acc ctt aaa gcc caa acc aag ctc gaa      672 
Leu His Leu Tyr Asn Ala Glu Thr Leu Lys Ala Gln Thr Lys Leu Glu 
    210                 215                 220 

tat ttc gcc gtt gac cgc gac agc cgg aaa ctg ctg att acc cct aaa      720 
Tyr Phe Ala Val Asp Arg Asp Ser Arg Lys Leu Leu Ile Thr Pro Lys 
225                 230                 235                 240 

acc gcc gcc tac gaa tcc caa tac caa gaa caa tac gcc ctt tgg acc      768 
Thr Ala Ala Tyr Glu Ser Gln Tyr Gln Glu Gln Tyr Ala Leu Trp Thr 
                245                 250                 255 

ggc cct tac aaa gtc agc aaa acc gtc aaa gcc tca gac cgc ctg atg      816 
Gly Pro Tyr Lys Val Ser Lys Thr Val Lys Ala Ser Asp Arg Leu Met 
            260                 265                 270 

gtc gat ttc tcc gac att acc ccc tac ggc gac aca acc gcc caa aac      864 
Val Asp Phe Ser Asp Ile Thr Pro Tyr Gly Asp Thr Thr Ala Gln Asn 
        275                 280                 285 

cgt ccc gac ttc aaa caa aac aac ggt aaa aaa ccc gat gtc ggc aac      912 
Arg Pro Asp Phe Lys Gln Asn Asn Gly Lys Lys Pro Asp Val Gly Asn 
    290                 295                 300 

gaa gtc atc cgc cgc cgc aaa gga gga                                  939 
Glu Val Ile Arg Arg Arg Lys Gly Gly 
305                 310 

 
           
             70  
             313  
             PRT  
             Neisseria meningitidis  
           
            70 

Met Lys Thr Leu Leu Leu Leu Ile Pro Leu Val Leu Thr Ala Cys Gly 
1               5                   10                  15 

Thr Leu Thr Gly Ile Pro Ala His Gly Gly Gly Lys Arg Phe Ala Val 
            20                  25                  30 

Glu Gln Glu Leu Val Ala Ala Ser Ser Arg Ala Ala Val Lys Glu Met 
        35                  40                  45 

Asp Leu Ser Ala Leu Lys Gly Arg Lys Ala Ala Leu Tyr Val Ser Val 
    50                  55                  60 

Met Gly Asp Gln Gly Ser Gly Asn Ile Ser Gly Gly Arg Tyr Ser Ile 
65                  70                  75                  80 

Asp Ala Leu Ile Arg Gly Gly Tyr His Asn Asn Pro Glu Ser Ala Thr 
                85                  90                  95 

Gln Tyr Ser Tyr Pro Ala Tyr Asp Thr Thr Ala Thr Thr Lys Ser Asp 
            100                 105                 110 

Ala Leu Ser Ser Val Thr Thr Ser Thr Ser Leu Leu Asn Ala Pro Ala 
        115                 120                 125 

Ala Ala Leu Thr Lys Asn Ser Gly Arg Lys Gly Glu Arg Ser Ala Gly 
    130                 135                 140 

Leu Ser Val Asn Gly Thr Gly Asp Tyr Arg Asn Glu Thr Leu Leu Ala 
145                 150                 155                 160 

Asn Pro Arg Asp Val Ser Phe Leu Thr Asn Leu Ile Gln Thr Val Phe 
                165                 170                 175 

Tyr Leu Arg Gly Ile Glu Val Val Pro Pro Glu Tyr Ala Asp Thr Asp 
            180                 185                 190 

Val Phe Val Thr Val Asp Val Phe Gly Thr Val Arg Ser Arg Thr Glu 
        195                 200                 205 

Leu His Leu Tyr Asn Ala Glu Thr Leu Lys Ala Gln Thr Lys Leu Glu 
    210                 215                 220 

Tyr Phe Ala Val Asp Arg Asp Ser Arg Lys Leu Leu Ile Thr Pro Lys 
225                 230                 235                 240 

Thr Ala Ala Tyr Glu Ser Gln Tyr Gln Glu Gln Tyr Ala Leu Trp Thr 
                245                 250                 255 

Gly Pro Tyr Lys Val Ser Lys Thr Val Lys Ala Ser Asp Arg Leu Met 
            260                 265                 270 

Val Asp Phe Ser Asp Ile Thr Pro Tyr Gly Asp Thr Thr Ala Gln Asn 
        275                 280                 285 

Arg Pro Asp Phe Lys Gln Asn Asn Gly Lys Lys Pro Asp Val Gly Asn 
    290                 295                 300 

Glu Val Ile Arg Arg Arg Lys Gly Gly 
305                 310 

 
           
             71  
             1308  
             DNA  
             Neisseria meningitidis  
             
               CDS  
               (1)..(1308)  
               NMB1876  N-acetyl glutamate synthetase  
             
           
            71 

atg agc gcg ccc gac ctc ttt gtc gcc cac ttc cgc gaa gcc gtc ccc       48 
Met Ser Ala Pro Asp Leu Phe Val Ala His Phe Arg Glu Ala Val Pro 
1               5                   10                  15 

tac atc cgc caa atg cgc ggc aaa acg ctg gtc gcc ggc ata gac gac       96 
Tyr Ile Arg Gln Met Arg Gly Lys Thr Leu Val Ala Gly Ile Asp Asp 
            20                  25                  30 

cgc ctg ctc gaa ggt gat acc tta aac aag ctc gcc gcc gac atc ggg      144 
Arg Leu Leu Glu Gly Asp Thr Leu Asn Lys Leu Ala Ala Asp Ile Gly 
        35                  40                  45 

ctg ttg tcg caa ctg ggc atc agg ctc gtc ctc atc cac ggc gcg cgc      192 
Leu Leu Ser Gln Leu Gly Ile Arg Leu Val Leu Ile His Gly Ala Arg 
    50                  55                  60 

cac ttc ctc gac cgc cac gcc gcc gct caa ggc cgc acg ccg cat tat      240 
His Phe Leu Asp Arg His Ala Ala Ala Gln Gly Arg Thr Pro His Tyr 
65                  70                  75                  80 

tgc cgg ggc ttg cgc gtt acc gac gaa acc tcg ctc gaa cag gcg cag      288 
Cys Arg Gly Leu Arg Val Thr Asp Glu Thr Ser Leu Glu Gln Ala Gln 
                85                  90                  95 

cag ttt gcc ggc acc gtc cgc agc cgt ttt gaa gcc gca ttg tgc ggc      336 
Gln Phe Ala Gly Thr Val Arg Ser Arg Phe Glu Ala Ala Leu Cys Gly 
            100                 105                 110 

agc gtt tcc ggg ttc gcg cgc gcg cct tcc gtc ccg ctc gta tcg ggc      384 
Ser Val Ser Gly Phe Ala Arg Ala Pro Ser Val Pro Leu Val Ser Gly 
        115                 120                 125 

aac ttc ctg acc gcc cgt ccg ata ggt gtg att gac gga acc gat atg      432 
Asn Phe Leu Thr Ala Arg Pro Ile Gly Val Ile Asp Gly Thr Asp Met 
    130                 135                 140 

gaa tac gcg ggc gtt atc cgc aaa acc gac acc gcc gcc ctc cgt ttc      480 
Glu Tyr Ala Gly Val Ile Arg Lys Thr Asp Thr Ala Ala Leu Arg Phe 
145                 150                 155                 160 

caa ctc gac gcg ggc aat atc gtc tgg ctg ccg ccg ctc gga cat tcc      528 
Gln Leu Asp Ala Gly Asn Ile Val Trp Leu Pro Pro Leu Gly His Ser 
                165                 170                 175 

tac agc ggc aag acc ttc tat ctc gat atg ctt caa acc gcc gcc tcc      576 
Tyr Ser Gly Lys Thr Phe Tyr Leu Asp Met Leu Gln Thr Ala Ala Ser 
            180                 185                 190 

gcc gcc gtc tcg ctt cag gcc gaa aaa ctc gtt tac ctg acc ctt tca      624 
Ala Ala Val Ser Leu Gln Ala Glu Lys Leu Val Tyr Leu Thr Leu Ser 
        195                 200                 205 

gac ggc att tcc cgc ccc gac ggc acg ctc gcc gaa acc ctc tcg gca      672 
Asp Gly Ile Ser Arg Pro Asp Gly Thr Leu Ala Glu Thr Leu Ser Ala 
    210                 215                 220 

cag gaa gcg caa tcg ctg gcg gaa cac gcc ggc ggc gaa acg cga cgg      720 
Gln Glu Ala Gln Ser Leu Ala Glu His Ala Gly Gly Glu Thr Arg Arg 
225                 230                 235                 240 

ctg att tcg tcc gcc gtt gcc gcg ctc gaa ggc ggc gtg cat cgc gtc      768 
Leu Ile Ser Ser Ala Val Ala Ala Leu Glu Gly Gly Val His Arg Val 
                245                 250                 255 

caa atc ctc aac gga gcc gcc gac ggc agc ctg ctg caa gaa ctc ttc      816 
Gln Ile Leu Asn Gly Ala Ala Asp Gly Ser Leu Leu Gln Glu Leu Phe 
            260                 265                 270 

acc cgc aac ggc atc ggc acg tcc att gcc aaa gaa gcc ttc gtc tcc      864 
Thr Arg Asn Gly Ile Gly Thr Ser Ile Ala Lys Glu Ala Phe Val Ser 
        275                 280                 285 

atc cgg cag gcg cac agc ggc gac atc ccg cac atc gcc gcc ctc atc      912 
Ile Arg Gln Ala His Ser Gly Asp Ile Pro His Ile Ala Ala Leu Ile 
    290                 295                 300 

cgc ccg ctg gaa gaa cag ggc atc ctg ctg cac cgc agc cgc gaa tac      960 
Arg Pro Leu Glu Glu Gln Gly Ile Leu Leu His Arg Ser Arg Glu Tyr 
305                 310                 315                 320 

ctc gaa aac cac att tcc gaa ttt tcc atc ctc gaa cac gac ggc aac     1008 
Leu Glu Asn His Ile Ser Glu Phe Ser Ile Leu Glu His Asp Gly Asn 
                325                 330                 335 

ctg tac ggt tgc gcc gcc ctg aaa acc ttt gcc gaa gcc gat tgc ggc     1056 
Leu Tyr Gly Cys Ala Ala Leu Lys Thr Phe Ala Glu Ala Asp Cys Gly 
            340                 345                 350 

gaa atc gcc tgc ctt gcc gtc tcg ccg cag gca cag gac ggc ggc tac     1104 
Glu Ile Ala Cys Leu Ala Val Ser Pro Gln Ala Gln Asp Gly Gly Tyr 
        355                 360                 365 

ggc gaa cgc ctg ctt gcc cac att atc gat aag gcg cgc ggc ata ggc     1152 
Gly Glu Arg Leu Leu Ala His Ile Ile Asp Lys Ala Arg Gly Ile Gly 
    370                 375                 380 

ata agc agg ctg ttc gca ctg tcc aca aat acc ggc gaa tgg ttt gcc     1200 
Ile Ser Arg Leu Phe Ala Leu Ser Thr Asn Thr Gly Glu Trp Phe Ala 
385                 390                 395                 400 

gaa cgc ggc ttt cag acg gca tcg gaa gac gag ttg ccc gaa acg cgg     1248 
Glu Arg Gly Phe Gln Thr Ala Ser Glu Asp Glu Leu Pro Glu Thr Arg 
                405                 410                 415 

cgc aaa gac tac cgc agc aac gga cgg aac tcg cat att ctg gta cgt     1296 
Arg Lys Asp Tyr Arg Ser Asn Gly Arg Asn Ser His Ile Leu Val Arg 
            420                 425                 430 

cgc ctg cac cgc                                                     1308 
Arg Leu His Arg 
        435 

 
           
             72  
             436  
             PRT  
             Neisseria meningitidis  
           
            72 

Met Ser Ala Pro Asp Leu Phe Val Ala His Phe Arg Glu Ala Val Pro 
1               5                   10                  15 

Tyr Ile Arg Gln Met Arg Gly Lys Thr Leu Val Ala Gly Ile Asp Asp 
            20                  25                  30 

Arg Leu Leu Glu Gly Asp Thr Leu Asn Lys Leu Ala Ala Asp Ile Gly 
        35                  40                  45 

Leu Leu Ser Gln Leu Gly Ile Arg Leu Val Leu Ile His Gly Ala Arg 
    50                  55                  60 

His Phe Leu Asp Arg His Ala Ala Ala Gln Gly Arg Thr Pro His Tyr 
65                  70                  75                  80 

Cys Arg Gly Leu Arg Val Thr Asp Glu Thr Ser Leu Glu Gln Ala Gln 
                85                  90                  95 

Gln Phe Ala Gly Thr Val Arg Ser Arg Phe Glu Ala Ala Leu Cys Gly 
            100                 105                 110 

Ser Val Ser Gly Phe Ala Arg Ala Pro Ser Val Pro Leu Val Ser Gly 
        115                 120                 125 

Asn Phe Leu Thr Ala Arg Pro Ile Gly Val Ile Asp Gly Thr Asp Met 
    130                 135                 140 

Glu Tyr Ala Gly Val Ile Arg Lys Thr Asp Thr Ala Ala Leu Arg Phe 
145                 150                 155                 160 

Gln Leu Asp Ala Gly Asn Ile Val Trp Leu Pro Pro Leu Gly His Ser 
                165                 170                 175 

Tyr Ser Gly Lys Thr Phe Tyr Leu Asp Met Leu Gln Thr Ala Ala Ser 
            180                 185                 190 

Ala Ala Val Ser Leu Gln Ala Glu Lys Leu Val Tyr Leu Thr Leu Ser 
        195                 200                 205 

Asp Gly Ile Ser Arg Pro Asp Gly Thr Leu Ala Glu Thr Leu Ser Ala 
    210                 215                 220 

Gln Glu Ala Gln Ser Leu Ala Glu His Ala Gly Gly Glu Thr Arg Arg 
225                 230                 235                 240 

Leu Ile Ser Ser Ala Val Ala Ala Leu Glu Gly Gly Val His Arg Val 
                245                 250                 255 

Gln Ile Leu Asn Gly Ala Ala Asp Gly Ser Leu Leu Gln Glu Leu Phe 
            260                 265                 270 

Thr Arg Asn Gly Ile Gly Thr Ser Ile Ala Lys Glu Ala Phe Val Ser 
        275                 280                 285 

Ile Arg Gln Ala His Ser Gly Asp Ile Pro His Ile Ala Ala Leu Ile 
    290                 295                 300 

Arg Pro Leu Glu Glu Gln Gly Ile Leu Leu His Arg Ser Arg Glu Tyr 
305                 310                 315                 320 

Leu Glu Asn His Ile Ser Glu Phe Ser Ile Leu Glu His Asp Gly Asn 
                325                 330                 335 

Leu Tyr Gly Cys Ala Ala Leu Lys Thr Phe Ala Glu Ala Asp Cys Gly 
            340                 345                 350 

Glu Ile Ala Cys Leu Ala Val Ser Pro Gln Ala Gln Asp Gly Gly Tyr 
        355                 360                 365 

Gly Glu Arg Leu Leu Ala His Ile Ile Asp Lys Ala Arg Gly Ile Gly 
    370                 375                 380 

Ile Ser Arg Leu Phe Ala Leu Ser Thr Asn Thr Gly Glu Trp Phe Ala 
385                 390                 395                 400 

Glu Arg Gly Phe Gln Thr Ala Ser Glu Asp Glu Leu Pro Glu Thr Arg 
                405                 410                 415 

Arg Lys Asp Tyr Arg Ser Asn Gly Arg Asn Ser His Ile Leu Val Arg 
            420                 425                 430 

Arg Leu His Arg 
        435 

 
           
             73  
             712  
             PRT  
             Neisseria meningitidis  
             
               MISC_FEATURE  
               TbpB  
             
           
            73 

Met Asn Asn Pro Leu Val Asn Gln Ala Ala Met Val Leu Pro Val Phe 
1               5                   10                  15 

Leu Leu Ser Ala Cys Leu Gly Gly Gly Gly Ser Phe Asp Leu Asp Ser 
            20                  25                  30 

Val Asp Thr Glu Ala Pro Arg Pro Ala Pro Lys Tyr Gln Asp Val Phe 
        35                  40                  45 

Ser Glu Lys Pro Gln Ala Gln Lys Asp Gln Gly Gly Tyr Gly Phe Ala 
    50                  55                  60 

Met Arg Leu Lys Arg Arg Asn Trp Tyr Pro Gln Ala Lys Glu Asp Glu 
65                  70                  75                  80 

Val Lys Leu Asp Glu Ser Asp Trp Glu Ala Thr Gly Leu Pro Asp Glu 
                85                  90                  95 

Pro Lys Glu Leu Pro Lys Arg Gln Lys Ser Val Ile Glu Lys Val Glu 
            100                 105                 110 

Thr Asp Ser Asp Asn Asn Ile Tyr Ser Ser Pro Tyr Leu Lys Pro Ser 
        115                 120                 125 

Asn His Gln Asn Gly Asn Thr Gly Asn Gly Ile Asn Gln Pro Lys Asn 
    130                 135                 140 

Gln Ala Lys Asp Tyr Glu Asn Phe Lys Tyr Val Tyr Ser Gly Trp Phe 
145                 150                 155                 160 

Tyr Lys His Ala Lys Arg Glu Phe Asn Leu Lys Val Glu Pro Lys Ser 
                165                 170                 175 

Ala Lys Asn Gly Asp Asp Gly Tyr Ile Phe Tyr His Gly Lys Glu Pro 
            180                 185                 190 

Ser Arg Gln Leu Pro Ala Ser Gly Lys Ile Thr Tyr Lys Gly Val Trp 
        195                 200                 205 

His Phe Ala Thr Asp Thr Lys Lys Gly Gln Lys Phe Arg Glu Ile Ile 
    210                 215                 220 

Gln Pro Ser Lys Ser Gln Gly Asp Arg Tyr Ser Gly Phe Ser Gly Asp 
225                 230                 235                 240 

Asp Gly Glu Glu Tyr Ser Asn Lys Asn Lys Ser Thr Leu Thr Asp Gly 
                245                 250                 255 

Gln Glu Gly Tyr Gly Phe Thr Ser Asn Leu Glu Val Asp Phe His Asn 
            260                 265                 270 

Lys Lys Leu Thr Gly Lys Leu Ile Arg Asn Asn Ala Asn Thr Asp Asn 
        275                 280                 285 

Asn Gln Ala Thr Thr Thr Gln Tyr Tyr Ser Leu Glu Ala Gln Val Thr 
    290                 295                 300 

Gly Asn Arg Phe Asn Gly Lys Ala Thr Ala Thr Asp Lys Pro Gln Gln 
305                 310                 315                 320 

Asn Ser Glu Thr Lys Glu His Pro Phe Val Ser Asp Ser Ser Ser Leu 
                325                 330                 335 

Ser Gly Gly Phe Phe Gly Pro Gln Gly Glu Glu Leu Gly Phe Arg Phe 
            340                 345                 350 

Leu Ser Asp Asp Gln Lys Val Ala Val Val Gly Ser Ala Lys Thr Lys 
        355                 360                 365 

Asp Lys Pro Ala Asn Gly Asn Thr Ala Ala Ala Ser Gly Gly Thr Asp 
    370                 375                 380 

Ala Ala Ala Ser Asn Gly Ala Ala Gly Thr Ser Ser Glu Asn Gly Lys 
385                 390                 395                 400 

Leu Thr Thr Val Leu Asp Ala Val Glu Leu Lys Leu Gly Asp Lys Glu 
                405                 410                 415 

Val Gln Lys Leu Asp Asn Phe Ser Asn Ala Ala Gln Leu Val Val Asp 
            420                 425                 430 

Gly Ile Met Ile Pro Leu Leu Pro Glu Ala Ser Glu Ser Gly Asn Asn 
        435                 440                 445 

Gln Ala Asn Gln Gly Thr Asn Gly Gly Thr Ala Phe Thr Arg Lys Phe 
    450                 455                 460 

Asp His Thr Pro Glu Ser Asp Lys Lys Asp Ala Gln Ala Gly Thr Gln 
465                 470                 475                 480 

Thr Asn Gly Ala Gln Thr Ala Ser Asn Thr Ala Gly Asp Thr Asn Gly 
                485                 490                 495 

Lys Thr Lys Thr Tyr Glu Val Glu Val Cys Cys Ser Asn Leu Asn Tyr 
            500                 505                 510 

Leu Lys Tyr Gly Met Leu Thr Arg Lys Asn Ser Lys Ser Ala Met Gln 
        515                 520                 525 

Ala Gly Glu Ser Ser Ser Gln Ala Asp Ala Lys Thr Glu Gln Val Glu 
    530                 535                 540 

Gln Ser Met Phe Leu Gln Gly Glu Arg Thr Asp Glu Lys Glu Ile Pro 
545                 550                 555                 560 

Ser Glu Gln Asn Ile Val Tyr Arg Gly Ser Trp Tyr Gly Tyr Ile Ala 
                565                 570                 575 

Asn Asp Lys Ser Thr Ser Trp Ser Gly Asn Ala Ser Asn Ala Thr Ser 
            580                 585                 590 

Gly Asn Arg Ala Glu Phe Thr Val Asn Phe Ala Asp Lys Lys Ile Thr 
        595                 600                 605 

Gly Thr Leu Thr Ala Asp Asn Arg Gln Glu Ala Thr Phe Thr Ile Asp 
    610                 615                 620 

Gly Asn Ile Lys Asp Asn Gly Phe Glu Gly Thr Ala Lys Thr Ala Glu 
625                 630                 635                 640 

Ser Gly Phe Asp Leu Asp Gln Ser Asn Thr Thr Arg Thr Pro Lys Ala 
                645                 650                 655 

Tyr Ile Thr Asp Ala Lys Val Gln Gly Gly Phe Tyr Gly Pro Lys Ala 
            660                 665                 670 

Glu Glu Leu Gly Gly Trp Phe Ala Tyr Pro Gly Asp Lys Gln Thr Lys 
        675                 680                 685 

Asn Ala Thr Asn Ala Ser Gly Asn Ser Ser Ala Thr Val Val Phe Gly 
    690                 695                 700 

Ala Lys Arg Gln Gln Pro Val Arg 
705                 710 

 
           
             74  
             67  
             DNA  
             Neisseria meningitidis  
             
               misc_feature  
               porA promoter sequence  
             
           
            74 

ttgaagacgt atcgggtgtt tgcccgatgt ttttaggttt ttatcaaatt tacaaaagga     60 

agccgat                                                               67 

 
           
             75  
             253  
             PRT  
             unidentified  
             
               MISC_FEATURE  
               kanamycin resistance  
             
           
            75 

Met Asn Gly Pro Ile Ile Met Thr Arg Glu Glu Arg Met Lys Ile Val 
1               5                   10                  15 

His Glu Ile Lys Glu Arg Ile Leu Asp Lys Tyr Gly Asp Asp Val Lys 
            20                  25                  30 

Ala Ile Gly Val Tyr Gly Ser Leu Gly Arg Gln Thr Asp Gly Pro Tyr 
        35                  40                  45 

Ser Asp Ile Glu Met Met Cys Val Met Ser Thr Glu Glu Ala Glu Phe 
    50                  55                  60 

Ser His Glu Trp Thr Thr Gly Glu Trp Lys Val Glu Val Asn Phe Asp 
65                  70                  75                  80 

Ser Glu Glu Ile Leu Leu Asp Tyr Ala Ser Gln Val Glu Ser Asp Trp 
                85                  90                  95 

Pro Leu Thr His Gly Gln Phe Phe Ser Ile Leu Pro Ile Tyr Asp Ser 
            100                 105                 110 

Gly Gly Tyr Leu Glu Lys Val Tyr Gln Thr Ala Lys Ser Val Glu Ala 
        115                 120                 125 

Gln Thr Phe His Asp Ala Ile Cys Ala Leu Ile Val Glu Glu Leu Phe 
    130                 135                 140 

Glu Tyr Ala Gly Lys Trp Arg Asn Ile Arg Val Gln Gly Pro Thr Thr 
145                 150                 155                 160 

Phe Leu Pro Ser Leu Thr Val Gln Val Ala Met Ala Gly Ala Met Leu 
                165                 170                 175 

Ile Gly Leu His His Arg Ile Cys Tyr Thr Thr Ser Ala Ser Val Leu 
            180                 185                 190 

Thr Glu Ala Val Lys Gln Ser Asp Leu Pro Ser Gly Tyr Asp His Leu 
        195                 200                 205 

Cys Gln Phe Val Met Ser Gly Gln Leu Ser Asp Ser Glu Lys Leu Leu 
    210                 215                 220 

Glu Ser Leu Glu Asn Phe Trp Asn Gly Ile Gln Glu Trp Thr Glu Arg 
225                 230                 235                 240 

His Gly Tyr Ile Val Asp Val Ser Lys Arg Ile Pro Phe 
                245                 250