Abstract:
The present invention relates to the use of natural or purified lipids or fats extracted from the fruit of a palm tree of the genus  Astrocaryum , for increasing dermic and/or capillary hydration. The palm fats of the present invention may be used in hygiene products, cosmetics and pharmaceutical products. The invention also includes compositions containing the palm fats obtained according to the invention.

Description:
RELATED APPLICATIONS 
     This application claims priority to Brazilian patent application number PI0106625-0 filed Oct. 8, 2001, the entirety of which is incorporated herein by reference. 
     1. Field of the Invention 
     The present invention relates to the use of natural or purified and stable vegetable fat extracted from the fruit of the palm tree of the genus  Astrocaryum  for increasing dermal and/or capillary hydration/emollience, and compositions containing such fats. The material palm fats extracted according to the invention may be used in hygiene products, cosmetics and pharmaceutical products. 
     2. Background 
     Vegetable oils and fats usually have a hydrating/emollient action. Vegetable and animal fats and oils have long been known to be emollients (Berdick, 1972), as there are records of such use in cosmetics since 7000 B.C. (Denavarre, 1978). These lipidic compounds have a soothing effect on the skin and make the hair easier to comb. Vegetable oils and fats usually surpass animal oil and fats, keeping the advantage of being emollient, making the hair easier to comb, being safe and providing a soothing effect with less of a greasy feel (Berdick, 1972). 
     Emollients are substances that maintain skin&#39;s softness, smoothness and flexibility, having a protective effect against dehydration and irritation, forming a barrier that prevents the excessive loss of cutaneous moisture (Hamilton, 1971). In addition to these two known hydration, occlusion and humidifying mechanisms, a third mechanisms has been recently proposed, the intercellular lipids stabilization mechanism in the lamellar crystalline liquid phases. The fats and the oils or products that may act according to these mechanisms provide a longer lasting hydration (Barker, 1992). 
     Lipids commonly used in hydrating products may reduce the reaction of irritating agents in the skin (Loden and Anderson, 1996). The incorporation of these humidifying agents as topical use ingredients provides protection effects and substitution of some of the skin&#39;s lipidic layers (SCHAICH and KORTING, 1992). 
     The use of physiologic lipids lead to new forms of topical therapies for dermatoses (such as psoriasis, atopic dermatitis, and irritating dermatitis) caused by the abnormal functioning of the lipidic barrier (Feingold, et al., 1996). 
     SUMMARY OF THE INVENTION 
     The present invention comprises natural fats extracted from the fruits of a palm tree of the genus  Astrocaryum , and their use in compositions and methods for increasing skin emolliance and/or hydration. The present invention is based on the surprising discovery that fats from  Astrocaryum  palm trees can increase in the skin&#39;s hydration potential up to ninety-four point eight percent (94.8%). 
    
    
     
       BRIEF DESCRIPTION OF THE FIGURES 
         FIG. 1  is a graph illustrating the increase in the tewametric index using compositions of the present invention. 
         FIG. 2  is a graph illustrating the improvement in the cutaneous barrier using compositions of the present invention. 
     
    
    
     DETAILED DESCRIPTION 
     The fruits of the palm tree of the genus  Astrocaryum  have two parts—pulp and seeds—from which it is possible to obtain crude oils and fats by cold extraction, e.g., using hydraulic or continuous screw presses (expellers), or also by the traditional extraction method using boiling water and removing the oil or floating fat. The pulp may be processed dry or wet. 
     Crude fat from the fruit of the palm tree of the genus  Astrocaryum  can be obtained by cold pressing. The seed of the fruit is composed by a hard wooden shell and an inner almond that is removed after the shells are broken. The whole almond, either broken or ground, is subject to a cold extraction process in hydraulic or continuous screw presses (expellers), or further by the traditional extraction method using boiling water and removing the oil or floating fat, thus obtaining the crude fat. The crude fat then can be refined to obtain the fats of the present invention. 
     In a preferred embodiment, the crude fat from the fruit of the palm tree of the genus  Astrocaryum  is further refined according to the following process:
     Step 1—De-gumming—if necessary, upon addition of two percent (2%) of water (70° C.) under the fat at 70° C.–80° C., agitating for 10–15 minutes, followed by centrifuging and removal of the heavy phase (gum).   Step 2—Neutralization—with NaOH aqueous solution, at 16–18° Be (Baumé), added to the oil at 70–80° C., agitating for 10–15 minutes, followed by centrifuging and removal of the heavy phase (sediments).   Step 3—Wash—with hot water (80–95° C.), two or three times, if necessary.   Step 4—Drying—under vacuum at 70–90° C.   Step 5—Bleaching—with bleaching clay (0.2–2%), under vacuum at 70–90° C. for 10–15 minutes, followed by filtering and cooling.   Step 6—Deodorization—under vacuum and N 2  current or water vapor at 120–180° C. for 1–5 hours, cool under vacuum to 45–50° C. and unload the reactor. Analyses: Acidity. Peroxide, color, stability.   

     Using the  Astrocaryum murumuru  and  Astrocaryum tucumã  species as an example, the typical physio-chemical and microbiologic characteristics of purified vegetable fat derived from  Astrocaryum  trees are as follows: 
     
       
         
               
               
               
               
             
               
               
               
               
               
               
             
               
               
               
               
             
               
               
               
               
               
               
             
               
               
               
               
             
               
               
               
               
             
               
               
               
               
               
               
             
               
               
               
               
             
           
               
                   
               
             
             
               
                 Analyses 
                 Unit 
                 
                   Astrocaryum murumuru 
                 
                 
                   Astrocaryum tucumã 
                 
               
               
                   
               
               
                 Appearance (25° C.) 
                 — 
                 Fatty solid 
                 Fatty solid 
               
               
                 Color 
                 — 
                 White to light yellow 
                 White to light yellow 
               
               
                 Odor 
                 — 
                 Characteristic 
                 Characteristic 
               
             
          
           
               
                 Acidity Index 
                 mg KOH/g 
                 5.00 
                 max. * 
                 5.00 
                 max. * 
               
             
          
           
               
                 Saponification Index 
                 mg KOH/g 
                   200–270 * 
                   200–270 * 
               
             
          
           
               
                 Iodine Index 
                 g I2_/100 g 
                 24.0 
                 max. * 
                 24.0 
                 max. * 
               
               
                 Fusion Index 
                 ° C. 
                   
               
               
                 Non-saponifiable 
                 % p/p 
                 0.2 
                 min. 
                 0.2 
                 min. 
               
               
                 materials 
               
               
                 Peroxides Index 
                 mEq o2/1000 g 
                 12.0 
                 max. 
                 12.0 
                 max. 
               
               
                 Water Content (KF) 
                 % p/p 
                 0.30 
                 max. 
                 0.30 
                 max. 
               
               
                 Standard Plate Count 
                 UFC/ml 
                 100 
                 max. 
                 100 
                 max. 
               
             
          
           
               
                 Fecal Coliform Count 
                 UFC/ml 
                 Absent 
                 Absent 
               
               
                 
                   Escherichia coli 
                 
                 UFC/ml 
                 Absent 
                 Absent 
               
               
                 Molds and Yeasts 
                 UFC/ml 
                 Absent 
                 Absent 
               
               
                 
                   Salmonella spp 
                 
                 UFC/ml 
                 Absent 
                 Absent 
               
               
                 
                   Staphylococcus aureus 
                 
                 UFC/ml 
                 Absent 
                 Absent 
               
               
                 
                   Pseudomonas sp 
                 
                 UFC/ml 
                 Absent 
                 Absent 
               
               
                   
               
               
                 * Typical analysis 
               
             
          
           
               
                   
                   
                 % 
                 % 
               
               
                   
                 Fatty Acids 
                 
                   Astrocaryum murumuru 
                 
                 
                   Astrocaryum tucumã 
                 
               
               
                   
                   
               
             
          
           
               
                   
                  C8:0 
                 2.0 
                 max. 
                 2.0 
                 max. 
               
               
                   
                 C10:0 
                 2.0 
                 max. 
                 2.0 
                 max. 
               
             
          
           
               
                   
                 C12:0 
                 45–60 
                 45–60 
               
               
                   
                 C14:0 
                 20–30 
                 20–30 
               
               
                   
                 C16:0 
                 5.0–8.5 
                 5.0–8.5 
               
               
                   
                 C18:0 
                 1.0–3.5 
                 1.0–3.5 
               
               
                   
                 C18:1 
                  5.0–10.0 
                  5.0–10.0 
               
               
                   
                 C18:2 
                 2.0–4.0 
                 2.0–4.0 
               
               
                   
                   
               
             
          
         
       
     
     Fats derived from  Astrocaryum  trees comprise a combination of fatty acids, as exemplified in the table below. The fatty acids present in the highest concentration in the present purified vegetable fats of the genus  Astrocaryum  are lauric and myristic acids (medium-chain fatty acids). 
     Tests demonstrating the increase in hydration using the palm fats obtained as described above were carried out at EVIC Brasil, which is located at Av. Indianópolis, 1455—Planalto Paulista—04063-002—São Paulo. Tests for the evaluation of the Maintenance of the Cutaneous Barrier after a Single Application of a Cosmetic Product in Humans, considering the potential to obtain a cutaneous barrier forming and fully transcribed effect are described below. 
     PRODUCT STUDY/REFERENCE: EBb012b/01.034, 01.042, 01.043: EVALUATION OF THE MAINTENANCE OF THE CUTANEOUS BARRIER AFTER A SINGLE APPLICATION OF A COSMETIC PRODUCT IN HUMANS TEST PRODUCTS: Base Emulsion, 0.2%, 1% and 3%  Astrocaryum murumuru  emulsion 
     The skin is an external barrier of our organism that performs several functions: protection against physical damage and ultraviolet radiation, control of the entrance of external materials (such as water and microorganisms), control of the excretion of fluids, salts and other substances, thermoregulation, among others. 
     Water is a very important component of skin for the maintenance of its physical integrity, elasticity and other properties. 
     The water in  stratum corneum  comes form inferior layers such as the epidermis and the dermis, and evaporates into the atmosphere. 
     The skin&#39;s water retention capacity may be evaluated in several ways. In this study the transepidermal water loss was determined in order to evaluate each hydrating product&#39;s efficacy and to improve the skin&#39;s hydric conditions after a single application. 
     The study evaluates the effect of a cosmetic product on the loss of the skin&#39;s transepidermal water loss after a single application (forearm) under controlled conditions. 
     The study was monocentric and was openly carried out. The control was intraindividual. 
     The study was carried out according to the general conditions of EVIC International and those that are specific from the Investigation Center and that were established for the performance of tests in humans. 
                                                   Test product identification                3% Creamy   0.2% Creamy   1% Creamy               Emulsion   Emulsion   Emulsion   Creamy             Astrocaryum       Astrocaryum       Astrocaryum     Emulsion       Name     murumuru       murumuru       murumuru     Vehicle               Reference   01.034   01.041   01.042   01.043       Batch #   CL028B01   CL028E01   CL028A01   CL028D01       Galenic Form and   white emulsion   white emulsion   white emulsion   white emulsion       organoleptic           characteristics           Necessary   50 g/L   50 g/L   50 g/L   50 g/L       quantity / # of           packages                    
The formula for the products tested are described below.
 
Test Product Related Information
 
     There were 10 volunteers whose data were extrapolated in the end of the study. 
     The specific inclusion criteria were as follows:
         age: 18 to 54 years old   gender: male or female   phototype (Fitzpatrick): I to IV   dry and hairless skin in the front part of the forearm
 
Specific Non-inclusive Criteria:
       

     The specific non-inclusive criteria were as follows:
         cutaneous marks in the experimental area, which may interfere with the evaluation of cutaneous reactions (pigmentation problems, scars, overdeveloped pilosity, ephelis, and neavi in large quantity, sun burn . . . )   allergy or reactivity to the category of the product tested   treatment using a hydrating product in the experimental zone 2 weeks before the beginning of the study   treatment using acidic Vitamin A and its derivatives within 3 months before the beginning of the study
 
Experimental Areas
       

     The experimental areas are the front sides of the forearms: one randomly chosen forearm receives the product and the other serves as a non-treated control. 
     Product Application Conditions 
     
         
         
           
             treated area: The product is applied once by the technician in charge of the study using a latex finger cots with a light finger massage up to penetration in an outlined area of 25 cm 2 , at an amount of 2 mg/cm 2 , in the institute. 
             control area: An area of 25 cm 2  is outlined in the contralateral forearm in the same manner as in the treated side, but no product is applied.
 
Conditions Required by the Study
 
           
         
       
    
     The conditions imposed on the volunteers were as follows:
         no other product different from the one being tested should be applied to the experimental area   no anti-allergic or anti-inflammatory treatment and/or with acidic vitamin A (and its derivatives) in the day of the study
 
Instrumental Evaluation of the Hydrating Effect
       

     The hydration level of the skin&#39;s outer layers is quantitatively evaluated by the capacitance measures in different experimental periods. 
     The product&#39;s hydrating effect is evaluated by the hydration kinetic comparison gathered from two experimental areas. 
     All volunteers included in the study are considered in order to evaluate the test product&#39;s efficacy, so they are subject to all examinations in defined times. 
     Equipment 
     Transepidermic water loss (TEWL) is evaluated by a Tewameter TW210(Courage&amp;Khazaka), which has a probe with a pair of sensors for temperature and humidity. According to Fick&#39;s Law, the diffusion of water (mass by area in a determined period) is proportional to the concentration gradient of water in the probe. There is a variation in the TEWL measured when the skin&#39;s hydration varies. 
     Environmental Condition 
     The environmental conditions imposed to the volunteers 30 minutes before the beginning of measurements, and throughout the whole test were:
         Temperature: t°=20° C.+/−2° C.   Relative Humidity: RH=40%+/−15%
 
Measurement Sites
       

     The instrumental measurements are performed in documented and defined sites of the control and treated areas. 
     Measurement Rate 
     The measures are taken before the product is applied, 1 hour (T1), 2 hours (T2), 3 hours (T3) and 4 hours (T4) after application of the product. 
     Evaluation Criteria 
     The system&#39;s transepidermic water loss amounts are expressed in g/m 2 h. Expression and Interpretation of Results 
     The individual results are expressed in:
         absolute amounts in each experimental period,   variation rates in comparison with T0,   differences between the variation rates in comparison with T0 (control and treated areas).       

     The average and standard deviations are calculated. 
     The interpretation of the result is: 
     absolute, related to:
         the percentage of “reactive” volunteers: volunteers in whose difference between variation rates in comparison with T0 (control and treated areas) is equal to or exceeds 10%,   the rate of increase in transepidermic water loss under when effect of the product: at each experimental period (difference between variation rates in comparison with T0—control and treated areas) in “reactive” volunteers,   rate of improvement in cutaneous barrier formation under when effect of the product: at each experimental period (normalized difference between the rate of increase in the tewametric index between the base and the product) in “reactive” volunteers.       

     Table 1 below sets out the typological characteristics of the volunteers tested. 
     Results 
     The individual data (absolute amounts for each experimental period, variation rates in comparison with T0, differences between variation rates in comparison with T0 of the control and treated areas) and the statistic analysis details are included in Table 2A–2D. The randomizing table of the left/right sides is included in Table 3. 
     “Reactive” volunteer results are shown below for each product: 
     
       
         
               
             
               
               
             
               
               
               
               
               
             
               
               
               
               
               
             
               
               
             
               
             
               
               
             
               
               
               
               
               
             
               
               
               
               
               
             
               
               
             
               
             
               
               
             
               
               
               
               
               
             
               
               
               
               
               
             
               
               
             
               
             
               
               
             
               
               
               
               
               
             
               
               
               
               
               
             
               
               
             
           
               
                   
               
             
             
               
                 Formula 3 (01.034): 3.0%  Astrocaryum murumuru   
               
             
          
           
               
                 Variation of the tewametric indexes 
                 Experimental periods 
               
             
          
           
               
                 compared with T0 
                 T1 
                 T2 
                 T3 
                 T4 
               
               
                   
               
             
          
           
               
                 Treated area 
                 3.5 
                 8.0 
                 8.0 
                 8.7 
               
               
                 Control area 
                 −4.6 
                 −7.3 
                 −2.8 
                 1.6 
               
               
                 Difference between the control and 
                 4.7 
                 7.8 
                 8.0 
                 6.1 
               
               
                 treated area 
                   
               
               
                 % of “reactive” volunteers 
                 60 
                 60 
                 70 
                 70 
               
               
                   
               
             
          
           
               
                 Maximum effect in the increase of TEWL in 3 hours 
                 % of increase in 
               
               
                   
                 tewametric index = 8.0 
               
               
                 Remaining effect 4 hours after application 
                 % of increase in 
               
               
                   
                 tewametric index = 6.1 
               
               
                 Maximum effect in the cutaneous barrier 1 hour after application 
                 % of improvement in 
               
               
                   
                 cutaneous barrier = 94.8 
               
               
                   
               
             
          
           
               
                 Formula 1 (01.041): 0.2%  Astrocaryum murumuru   
               
             
          
           
               
                 Variation of the tewametric indexes 
                 Experimental periods 
               
             
          
           
               
                 compared with T0 
                 T1 
                 T2 
                 T3 
                 T4 
               
               
                   
               
             
          
           
               
                 Treated area 
                 51.5 
                 51.8 
                 50.9 
                 70.9 
               
               
                 Control area 
                 −4.3 
                 −6.7 
                 −2.5 
                 −9.5 
               
               
                 Difference between the control and 
                 55.7 
                 58.5 
                 53.4 
                 80.4 
               
               
                 treated area 
                   
               
               
                 % of “reactive” volunteers 
                 80 
                 90 
                 90 
                 90 
               
               
                   
               
             
          
           
               
                 Maximum effect in the increase of TEWL in 4 hours 
                 % of increase in 
               
               
                   
                 tewametric index = 80.4 
               
               
                 Remaining effect 4 hours after application 
                 % of increase in 
               
               
                   
                 tewametric index = 80.4 
               
               
                 Maximum effect in the cutaneous barrier 3 hours after application 
                 % of improvement in 
               
               
                   
                 cutaneous barrier = 29.3 
               
               
                   
               
             
          
           
               
                 Formula 2 (01.042): 1.0%  Astrocaryum murumuru   
               
             
          
           
               
                 Variation of the tewametric indexes 
                 Experimental periods 
               
             
          
           
               
                 compared with T0 
                 T1 
                 T2 
                 T3 
                 T4 
               
               
                   
               
             
          
           
               
                 Treated area 
                 44.7 
                 51.4 
                 48.5 
                 54.3 
               
               
                 Control area 
                 4.2 
                 2.2 
                 3.5 
                 8.3 
               
               
                 Difference between the control and 
                 40.5 
                 49.2 
                 45.0 
                 46.0 
               
               
                 treated area 
                   
               
               
                 % of “reactive” volunteers 
                 90 
                 80 
                 70 
                 80 
               
               
                   
               
             
          
           
               
                 Maximum effect in the increase of TEWL in 2 hours 
                 % of increase in 
               
               
                   
                 tewametric index = 49.2 
               
               
                 Remaining effect 4 hours after application 
                 % of increase in 
               
               
                   
                 tewametric index = 46.0 
               
               
                 Maximum effect in the cutaneous barrier 1 hour after application 
                 % of improvement in 
               
               
                   
                 cutaneous barrier = 54.7 
               
               
                   
               
             
          
           
               
                 Formula 4 (01.043): Base Emulsion 
               
             
          
           
               
                 Variation of the tewametric indexes 
                 Experimental periods 
               
             
          
           
               
                 compared with T0 
                 T1 
                 T2 
                 T3 
                 T4 
               
               
                   
               
             
          
           
               
                 Treated area 
                 91.1 
                 92.8 
                 87.4 
                 66.4 
               
               
                 Control area 
                 1.6 
                 5.2 
                 11.8 
                 5.3 
               
               
                 Difference between the control and 
                 89.5 
                 87.6 
                 75.6 
                 61.1 
               
               
                 treated area 
                   
               
               
                 % of “reactive” volunteers 
                 100 
                 100 
                 100 
                 90 
               
               
                   
               
             
          
           
               
                 Maximum effect in the increase of TEWL in 4 hours 
                 % of increase in 
               
               
                   
                 tewametric index = 61.1 
               
               
                 Remaining effect 4 hours after application 
                 % of increase in 
               
               
                   
                 tewametric index = 61.1 
               
               
                   
               
             
          
         
       
         
           FIG. 1  shows the % of increase in tewametric index 
           FIG. 2  shows the % of improvement in cutaneous barrier
 
Conclusions
 
       
    
     Under the experimental conditions adopted and taking into consideration the evolution of the instrumental parameters considered: 
     the 0.2%  Astrocaryum murumuru  emulsion (Formula 1) has a 29.3% cutaneous barrier forming effect (in relation to the base) in 90% of the panel 3 hours after application, 
     the 1.0%  Astrocaryum murumuru  emulsion (Formula 2) has a 54.7% cutaneous barrier forming effect (in relation to the base) in 90% of the panel 1 hour after application, 
     the 3.0%  Astrocaryum murumuru  emulsion (Formula 3) has a 94.8% cutaneous barrier forming effect (in relation to the base) in 60% of the panel 1 hour after application. 
     
       
         
               
             
               
               
               
               
             
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
             
               
             
               
             
               
               
               
               
               
             
           
               
                 TABLE 1 
               
               
                   
               
             
             
               
                 TYPOLOGICAL CHARACTERISTICS OF THE VOLUNTEERS 
               
             
          
           
               
                 Volunteers 
                   
                 Gender 
                   
               
             
          
           
               
                   
                 Name 
                   
                 M = Male 
                   
                 Dry skin 
                 Comeometric 
               
               
                 Ref. 
                 Last name 
                 Age 
                 F = Female 
                 Phototype* 
                 on forearm 
                 index on T0 
               
               
                   
               
             
          
           
               
                 1 
                 Tere C 
                 50 
                 F 
                 III 
                 X 
                 40 
               
               
                 2 
                 Izab J 
                 51 
                 F 
                 IV 
                 X 
                 36 
               
               
                 3 
                 Arad S 
                 40 
                 F 
                 IV 
                 X 
                 30 
               
               
                 4 
                 Dolo N 
                 61 
                 F 
                 IV 
                 X 
                 28 
               
               
                 5 
                 Patr F 
                 29 
                 F 
                 IV 
                 X 
                 44 
               
               
                 6 
                 Simo F 
                 19 
                 F 
                 III 
                 X 
                 36 
               
               
                 7 
                 Luiz M 
                 45 
                 F 
                 IV 
                 X 
                 27 
               
               
                 8 
                 Luiz S 
                 45 
                 F 
                 IV 
                 X 
                 47 
               
               
                 9 
                 Porf D 
                 45 
                 F 
                 IV 
                 X 
                 51 
               
               
                 10 
                 Egla C 
                 39 
                 F 
                 IV 
                 X 
                 38 
               
               
                   
               
             
          
           
               
                 Key: 
               
               
                 / = no 
               
               
                 X = yes 
               
               
                 *Phototype according to Fitzpatrick&#39;s classification. 
               
               
                   
               
             
          
           
               
                 Fitzpatrick&#39;s classification 
               
             
          
           
               
                 Type 
                 Hair 
                 Skin 
                 Freckles 
                 Sun burns 
               
               
                   
               
               
                 I 
                 red 
                 light 
                 +++ 
                 Constant 
               
               
                   
                   
                   
                   
                 no tanning 
               
               
                 II 
                 light 
                 light 
                 ++ 
                 Frequent 
               
               
                   
                   
                   
                   
                 tans lightly 
               
               
                 III 
                 light brown 
                 light 
                 + 
                 Inconsistent 
               
               
                   
                   
                   
                   
                 tans moderately 
               
               
                 IV 
                 dark 
                 brown 
                 0 
                 None 
               
               
                   
                   
                   
                   
                 considerable tanning 
               
               
                 V 
                 black and curly 
                 black 
                 0 
                 0 
               
               
                   
               
             
          
         
       
     
     
       
         
               
             
               
               
             
               
               
               
             
               
               
               
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
               
               
               
             
           
               
                 TABLE 2A.1 
               
             
             
               
                   
               
               
                 INSTRUMENTAL EVALUATION OF THE HYDRATING EFFECT 
               
               
                 tewametric indexes 
               
               
                 Product: 01.034 (Formula 3) 
               
             
          
           
               
                   
                 Control Area 
               
             
          
           
               
                   
                   
                 co V % 
               
               
                   
                   
                 Variation percentages in 
               
               
                 Volunteer&#39;s 
                 Absolute Values 
                 comparison with T0 
               
             
          
           
               
                 reference 
                 T0 
                 T1 
                 T2 
                 T3 
                 T4 
                 T1 
                 T2 
                 T3 
                 T4 
               
               
                   
               
             
          
           
               
                 1 
                 1.5 
                 1.0 
                 1.0 
                 1.3 
                 1.5 
                 −33.3 
                 −33.3 
                 −13.3 
                 0.0 
               
               
                 2 
                 1.4 
                 1.2 
                 1.2 
                 1.3 
                 1.0 
                 −14.3 
                 −14.3 
                 −7.1 
                 −28.6 
               
               
                 3 
                 1.0 
                 1.0 
                 1.0 
                 1.0 
                 1.3 
                 0.0 
                 0.0 
                 0.0 
                 30.0 
               
               
                 4 
                 4.5 
                 4.5 
                 4.6 
                 4.6 
                 4.6 
                 0.0 
                 2.2 
                 2.2 
                 2.2 
               
               
                 5 
                 4.4 
                 4.8 
                 4.5 
                 4.8 
                 4.6 
                 9.1 
                 2.3 
                 9.1 
                 4.5 
               
               
                 6 
                 4.8 
                 5.2 
                 5.0 
                 5.1 
                 5.3 
                 8.3 
                 4.2 
                 6.3 
                 10.4 
               
               
                 7 
                 1.8 
                 2.1 
                 1.9 
                 2.0 
                 1.9 
                 16.7 
                 5.6 
                 11.1 
                 5.6 
               
               
                 8 
                 7.5 
                 8.0 
                 7.9 
                 7.5 
                 7.4 
                 6.7 
                 5.3 
                 0.0 
                 −1.3 
               
               
                 9 
                 2.6 
                 2.8 
                 2.6 
                 2.3 
                 2.3 
                 7.7 
                 0.0 
                 −11.5 
                 −11.5 
               
               
                 10 
                 3.1 
                 2.8 
                 2.8 
                 3.0 
                 3.3 
                 −9.7 
                 −9.7 
                 −3.2 
                 6.5 
               
             
          
           
               
                 Vol. No. 
                 10 
                 10 
                 10 
                 10 
                 10 
                 10 
                 10 
                 10 
                 10 
               
               
                 Average 
                 3.3 
                 3.3 
                 3.3 
                 3.3 
                 3.3 
                 −0.9% 
                 −3.8% 
                 −0.7% 
                 1.8% 
               
               
                 Standard 
                 2.0 
                 2.3 
                 2.2 
                 2.1 
                 2.1 
                 14.7% 
                 12.3% 
                 8.3% 
                 15.1% 
               
               
                 Deviation 
               
               
                   
               
             
          
         
       
     
     
       
         
               
               
             
               
               
               
             
               
               
               
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
               
               
               
             
           
               
                   
                 TABLE 2A.2 
               
             
             
               
                   
                   
               
               
                   
                 Treated Area 
               
             
          
           
               
                   
                   
                 tr V % 
               
               
                   
                   
                 Variation percentages in 
               
               
                 Volunteer&#39;s 
                 Absolute Values 
                 comparison with T0 
               
             
          
           
               
                 reference 
                 T1 
                 T1 
                 T2 
                 T3 
                 T4 
                 T1 
                 T2 
                 T3 
                 T4 
               
               
                   
               
             
          
           
               
                 1 
                 1.2 
                 2.2 
                 2.4 
                 2.0 
                 2.2 
                 83.3 
                 100.0 
                 66.7 
                 83.3 
               
               
                 2 
                 0.6 
                 0.6 
                 0.7 
                 1.1 
                 1.0 
                 0.0 
                 16.7 
                 83.3 
                 66.7 
               
               
                 3 
                 2.5 
                 1.6 
                 1.5 
                 0.5 
                 0.5 
                 −36.0 
                 −40.0 
                 −80.0 
                 −80.0 
               
               
                 4 
                 4.0 
                 5.0 
                 5.0 
                 4.2 
                 4.3 
                 25.0 
                 25.0 
                 5.0 
                 7.5 
               
               
                 5 
                 3.6 
                 4.1 
                 4.0 
                 4.5 
                 4.8 
                 13.9 
                 11.1 
                 25.0 
                 33.3 
               
               
                 6 
                 4.8 
                 4.5 
                 4.5 
                 4.5 
                 4.6 
                 −6.3 
                 −6.3 
                 −6.3 
                 −4.2 
               
               
                 7 
                 1.5 
                 1.7 
                 1.8 
                 1.9 
                 1.5 
                 13.3 
                 20.0 
                 26.7 
                 0.0 
               
               
                 8 
                 7.0 
                 6.8 
                 7.0 
                 6.5 
                 6.1 
                 −2.9 
                 0.0 
                 −7.1 
                 −12.7 
               
               
                 9 
                 4.7 
                 3.4 
                 3.5 
                 3.5 
                 3.5 
                 −27.7 
                 −25.5 
                 −25.5 
                 −25.5 
               
               
                 10 
                 4.1 
                 3.0 
                 3.1 
                 3.2 
                 3.3 
                 −26.8 
                 −24.4 
                 −22.0 
                 −19.5 
               
             
          
           
               
                 Vol. No. 
                 10 
                 10 
                 10 
                 10 
                 10 
                 10 
                 10 
                 10 
                 10 
               
               
                 Average 
                 3.4 
                 3.3 
                 3.4 
                 3.2 
                 3.2 
                 3.6% 
                 7.7% 
                 6.6% 
                 4.9% 
               
               
                 Standard 
                 2.0 
                 1.9 
                 1.9 
                 1.8 
                 1.8 
                 34.4% 
                 39.1% 
                 47.1% 
                 47.1% 
               
               
                 Deviation 
               
               
                   
               
             
          
         
       
     
     
       
         
               
             
               
               
               
             
               
               
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                   
               
               
                 INSTRUMENTAL EVALUATION OF THE HYDRATING EFFECT 
               
               
                 tewametric indexes 
               
               
                 Product: 01.034 (Formula 3) 
               
               
                 Table 2A.3 
               
             
          
           
               
                 Volunteer&#39;s 
                 tr V − co V difference (%) 
                 Reactive 
               
             
          
           
               
                 reference 
                 T1 
                 T2 
                 T3 
                 T4 
                 volunteers* 
               
               
                   
               
             
          
           
               
                 1 
                 116.7 
                 133.3 
                 80.0 
                 83.3 
                 X 
               
               
                 2 
                 14.3 
                 31.0 
                 90.5 
                 95.2 
                 X 
               
               
                 3 
                 −36.0 
                 −40.0 
                 −80.0 
                 −110.0 
                 X 
               
               
                 4 
                 25.0 
                 22.8 
                 2.8 
                 5.3 
                 / 
               
               
                 5 
                 4.8 
                 8.8 
                 15.9 
                 28.8 
                 X 
               
               
                 6 
                 −14.6 
                 −10.4 
                 −12.5 
                 −14.6 
                 X 
               
               
                 7 
                 −3.3 
                 14.4 
                 15.6 
                 −5.6 
                 / 
               
               
                 8 
                 −9.5 
                 −5.3 
                 −7.1 
                 −11.4 
                 / 
               
               
                 9 
                 −35.4 
                 −25.5 
                 −14.0 
                 −14.0 
                 X 
               
               
                 10 
                 −17.2 
                 −14.7 
                 −18.7 
                 −26.0 
                 X 
               
               
                   
               
               
                 Key: 
               
               
                 / = no 
               
               
                 X = yes 
               
               
                 *volunteers whose difference between the variation percentage in comparison to T0 of treated and control areas is equal to or above 10% 
               
             
          
         
       
     
     
       
         
               
             
               
               
             
               
               
               
             
               
               
               
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
               
               
               
             
           
               
                 TABLE 2B 
               
             
             
               
                   
               
               
                 INSTRUMENTAL EVALUATION OF THE HYDRATING EFFECT 
               
               
                 tewametric indexes 
               
               
                 Product: 01.041 (Formula 1) 
               
               
                 Table 2B.1 
               
             
          
           
               
                   
                 Control Area 
               
             
          
           
               
                   
                   
                 co V % 
               
               
                   
                   
                 Variation percentages in 
               
               
                 Volunteer&#39;s 
                 Absolute Values 
                 comparison with T0 
               
             
          
           
               
                 reference 
                 T0 
                 T1 
                 T2 
                 T3 
                 T4 
                 T1 
                 T2 
                 T3 
                 T4 
               
               
                   
               
             
          
           
               
                 1 
                 1.1 
                 0.9 
                 0.8 
                 1.2 
                 1.0 
                 −18.2 
                 −27.3 
                 9.1 
                 −9.1 
               
               
                 2 
                 1.2 
                 1.2 
                 1.1 
                 1.2 
                 0.8 
                 0.0 
                 −8.3 
                 0.0 
                 −33.3 
               
               
                 3 
                 0.6 
                 0.8 
                 0.8 
                 0.8 
                 0.7 
                 33.3 
                 33.3 
                 33.3 
                 16.7 
               
               
                 4 
                 4.3 
                 4.3 
                 4.3 
                 4.5 
                 4.3 
                 0.0 
                 0.0 
                 4.7 
                 0.0 
               
               
                 5 
                 4.4 
                 4.1 
                 4.1 
                 4.4 
                 4.1 
                 −6.8 
                 −6.8 
                 0.0 
                 −6.8 
               
               
                 6 
                 4.0 
                 4.3 
                 4.0 
                 4.0 
                 4.4 
                 7.5 
                 0.0 
                 0.0 
                 10.0 
               
               
                 7 
                 1.6 
                 1.3 
                 1.3 
                 1.1 
                 1.0 
                 −18.8 
                 −18.8 
                 −37.5 
                 −37.5 
               
               
                 8 
                 6.7 
                 6.5 
                 7.0 
                 6.8 
                 6.1 
                 −3.0 
                 4.5 
                 1.5 
                 −9.0 
               
               
                 9 
                 2.5 
                 2.0 
                 2.0 
                 2.1 
                 2.2 
                 −20.0 
                 −20.0 
                 −16.0 
                 −12.0 
               
               
                 10 
                 2.4 
                 2.1 
                 2.0 
                 2.1 
                 2.3 
                 −12.5 
                 −16.7 
                 −12.5 
                 −4.2 
               
             
          
           
               
                 Vol. No. 
                 10 
                 10 
                 10 
                 10 
                 10 
                 10 
                 10 
                 10 
                 10 
               
               
                 Average 
                 2.9 
                 2.8 
                 2.7 
                 2.8 
                 2.7 
                 −3.8% 
                 −6.0% 
                 −1.7% 
                 −8.5% 
               
               
                 Standard 
                 1.9 
                 1.9 
                 2.0 
                 2.0 
                 1.9 
                 16.0% 
                 17.2% 
                 18.3% 
                 16.8% 
               
               
                 Deviation 
               
               
                   
               
             
          
         
       
     
     
       
         
               
               
             
               
               
               
             
               
               
               
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
               
               
               
             
           
               
                   
                 TABLE 2B.2 
               
             
             
               
                   
                   
               
               
                   
                 Treated Area 
               
             
          
           
               
                   
                   
                 tr V % 
               
               
                   
                   
                 Variation percentages in 
               
               
                 Volunteer&#39;s 
                 Absolute Values 
                 comparison with T0 
               
             
          
           
               
                 reference 
                 T1 
                 T1 
                 T2 
                 T3 
                 T4 
                 T1 
                 T2 
                 T3 
                 T4 
               
               
                   
               
             
          
           
               
                 1 
                 0.4 
                 0.7 
                 0.6 
                 0.6 
                 1.0 
                 75.0 
                 50.0 
                 50.0 
                 150.0 
               
               
                 2 
                 0.4 
                 0.5 
                 0.7 
                 0.6 
                 1.0 
                 25.0 
                 75.0 
                 50.0 
                 150.0 
               
               
                 3 
                 1.8 
                 1.1 
                 0.9 
                 0.5 
                 0.4 
                 −38.9 
                 −50.0 
                 −72.2 
                 −77.8 
               
               
                 4 
                 4.2 
                 3.8 
                 3.9 
                 4.0 
                 3.9 
                 −9.5 
                 −7.1 
                 −4.8 
                 −7.1 
               
               
                 5 
                 1.3 
                 2.4 
                 2.3 
                 2.5 
                 2.6 
                 84.6 
                 76.9 
                 92.3 
                 100.0 
               
               
                 6 
                 3.3 
                 3.5 
                 4.3 
                 4.7 
                 4.5 
                 6.1 
                 30.3 
                 42.4 
                 36.4 
               
               
                 7 
                 1.4 
                 2.0 
                 1.9 
                 1.8 
                 1.8 
                 42.9 
                 35.7 
                 28.6 
                 28.6 
               
               
                 8 
                 2.3 
                 5.1 
                 4.8 
                 5.0 
                 4.5 
                 121.7 
                 108.7 
                 117.4 
                 95.7 
               
               
                 9 
                 1.8 
                 3.0 
                 3.0 
                 3.3 
                 2.8 
                 66.7 
                 66.7 
                 83.3 
                 55.6 
               
               
                 10 
                 1.5 
                 2.7 
                 2.6 
                 2.5 
                 3.0 
                 80.0 
                 73.3 
                 66.7 
                 100.0 
               
             
          
           
               
                 Vol. No. 
                 10 
                 10 
                 10 
                 10 
                 10 
                 10 
                 10 
                 10 
                 10 
               
               
                 Average 
                 1.8 
                 2.5 
                 2.5 
                 2.6 
                 2.6 
                 45.4% 
                 45.9% 
                 45.4% 
                 63.1% 
               
               
                 Standard 
                 1.2 
                 1.5 
                 1.5 
                 1.7 
                 1.5 
                 49.4% 
                 49.4% 
                 53.7% 
                 71.2% 
               
               
                 Deviation 
               
               
                   
               
             
          
         
       
     
     
       
         
               
             
               
               
               
             
               
               
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                   
               
               
                 INSTRUMENTAL EVALUATION OF THE HYDRATING EFFECT 
               
               
                 tewametric indexes 
               
               
                 Product: 01.041 
               
               
                 Table 2B.3 
               
             
          
           
               
                 Volunteer&#39;s 
                 tr V 0 co V difference (%) 
                 Reactive 
               
             
          
           
               
                 reference 
                 T1 
                 T2 
                 T3 
                 T4 
                 volunteers* 
               
               
                   
               
             
          
           
               
                 1 
                 93.2 
                 77.3 
                 40.9 
                 159.1 
                 X 
               
               
                 2 
                 25.0 
                 83.3 
                 50.0 
                 183.3 
                 X 
               
               
                 3 
                 −72.2 
                 −83.3 
                 −105.6 
                 −94.4 
                 X 
               
               
                 4 
                 −9.5 
                 −7.1 
                 −9.4 
                 −7.1 
                 / 
               
               
                 5 
                 91.4 
                 83.7 
                 92.3 
                 106.8 
                 X 
               
               
                 6 
                 −1.4 
                 30.3 
                 42.4 
                 26.4 
                 X 
               
               
                 7 
                 61.6 
                 54.5 
                 66.1 
                 66.1 
                 X 
               
               
                 8 
                 124.7 
                 104.2 
                 115.9 
                 104.6 
                 X 
               
               
                 9 
                 86.7 
                 86.7 
                 99.3 
                 67.6 
                 X 
               
               
                 10 
                 92.5 
                 90.0 
                 79.2 
                 104.2 
                 X 
               
               
                   
               
               
                 Key: 
               
               
                 / = no 
               
               
                 X = yes 
               
               
                 *volunteers whose difference between the variation percentage in comparison to T0 of treated and control areas is equal to or above 10% 
               
             
          
         
       
     
     
       
         
               
             
               
               
             
               
               
               
             
               
               
               
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
               
               
               
             
           
               
                 TABLE 2C 
               
             
             
               
                   
               
               
                 INSTRUMENTAL EVALUATION OF THE HYDRATING EFFECT 
               
               
                 tewametric indexes 
               
               
                 Product 01.042 (Formula 2) 
               
               
                 Table 2C.1 
               
             
          
           
               
                   
                 Control Area 
               
             
          
           
               
                   
                   
                 co V % 
               
               
                   
                   
                 Variation percentages in 
               
               
                 Volunteer&#39;s 
                 Absolute Values 
                 comparison with T0 
               
             
          
           
               
                 reference 
                 T1 
                 T1 
                 T2 
                 T3 
                 T4 
                 T1 
                 T2 
                 T3 
                 T4 
               
               
                   
               
             
          
           
               
                 1 
                 1.6 
                 1.7 
                 1.8 
                 1.5 
                 2.0 
                 6.3 
                 12.5 
                 −6.3 
                 25.0 
               
               
                 2 
                 2.0 
                 2.1 
                 2.0 
                 2.0 
                 1.8 
                 5.0 
                 0.0 
                 0.0 
                 −10.0 
               
               
                 3 
                 1.4 
                 1.5 
                 1.5 
                 1.0 
                 1.5 
                 7.1 
                 7.1 
                 −28.6 
                 7.1 
               
               
                 4 
                 4.6 
                 4.0 
                 4.5 
                 4.5 
                 4.6 
                 −13.0 
                 −2.2 
                 −2.2 
                 0.0 
               
               
                 5 
                 2.6 
                 2.3 
                 2.5 
                 2.8 
                 2.8 
                 −11.5 
                 −2.3 
                 7.7 
                 7.7 
               
               
                 6 
                 4.4 
                 4.1 
                 4.5 
                 4.4 
                 4.4 
                 −6.8 
                 2.3 
                 0.0 
                 0.0 
               
               
                 7 
                 1.3 
                 1.6 
                 1.1 
                 1.6 
                 1.6 
                 23.1 
                 −15.4 
                 23.1 
                 23.1 
               
               
                 8 
                 4.1 
                 4.7 
                 4.5 
                 3.9 
                 4.2 
                 14.6 
                 9.8 
                 −4.9 
                 2.4 
               
               
                 9 
                 2.5 
                 2.3 
                 2.0 
                 2.4 
                 2.7 
                 −8.0 
                 −20.0 
                 −4.0 
                 8.0 
               
               
                 10 
                 2.0 
                 2.5 
                 2.6 
                 3.0 
                 2.4 
                 25.0 
                 30.0 
                 50.0 
                 20.0 
               
             
          
           
               
                 Vol. No. 
                 10 
                 10 
                 10 
                 10 
                 10 
                 10 
                 10 
                 10 
                 10 
               
               
                 Average 
                 2.7 
                 2.7 
                 2.7 
                 2.7 
                 2.8 
                 4.2% 
                 2.2% 
                 3.5% 
                 8.3% 
               
               
                 Standard 
                 1.3 
                 1.2 
                 1.3 
                 1.2 
                 1.2 
                 13.9% 
                 14.2% 
                 20.7% 
                 11.3% 
               
               
                 Deviation 
               
               
                   
               
             
          
         
       
     
     
       
         
               
               
             
               
               
               
             
               
               
               
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
               
               
               
             
           
               
                   
                 TABLE 2C.2 
               
             
             
               
                   
                   
               
               
                   
                 Treated Area 
               
             
          
           
               
                   
                   
                 tr V % 
               
               
                   
                   
                 Variation percentages in comparison 
               
               
                 Volunteer&#39;s 
                 Absolute Values 
                 with T0 
               
             
          
           
               
                 reference 
                 T0 
                 T1 
                 T2 
                 T3 
                 T4 
                 T1 
                 T2 
                 T3 
                 T4 
               
               
                   
               
             
          
           
               
                 1 
                 1.1 
                 1.1 
                 1.4 
                 1.8 
                 1.8 
                 0.0 
                 27.3 
                 63.6 
                 63.6 
               
               
                 2 
                 0.4 
                 1.7 
                 1.4 
                 1.4 
                 1.6 
                 325.0 
                 250.0 
                 250.0 
                 300.0 
               
               
                 3 
                 1.2 
                 1.0 
                 1.3 
                 1.3 
                 1.5 
                 −16.7 
                 8.3 
                 8.3 
                 25.0 
               
               
                 4 
                 3.2 
                 3.8 
                 4.6 
                 4.0 
                 3.8 
                 18.8 
                 43.8 
                 25.0 
                 18.8 
               
               
                 5 
                 2.8 
                 3.2 
                 3.8 
                 4.1 
                 3.7 
                 14.3 
                 35.7 
                 46.4 
                 32.1 
               
               
                 6 
                 3.4 
                 4.1 
                 5.0 
                 4.4 
                 3.8 
                 20.6 
                 47.1 
                 29.4 
                 11.8 
               
               
                 7 
                 1.2 
                 1.6 
                 1.8 
                 1.5 
                 1.5 
                 33.3 
                 50.0 
                 25.0 
                 25.0 
               
               
                 8 
                 8.0 
                 7.8 
                 8.0 
                 7.3 
                 7.1 
                 −2.5 
                 0.0 
                 −8.8 
                 −11.3 
               
               
                 9 
                 2.5 
                 2.6 
                 2.8 
                 2.4 
                 2.7 
                 4.0 
                 12.0 
                 −4.0 
                 8.0 
               
               
                 10 
                 2.0 
                 3.0 
                 2.8 
                 3.0 
                 3.4 
                 50.0 
                 40.0 
                 50.0 
                 70.0 
               
             
          
           
               
                 Vol. No. 
                 10 
                 10 
                 10 
                 10 
                 10 
                 10 
                 10 
                 10 
                 10 
               
               
                 Average 
                 2.6 
                 3.0 
                 3.3 
                 3.1 
                 3.1 
                 44.7% 
                 51.4% 
                 48.5% 
                 54.3% 
               
               
                 Standard 
                 2.1 
                 2.0 
                 2.1 
                 1.9 
                 1.7 
                 100.3% 
                 71.8% 
                 74.5% 
                 89.7% 
               
               
                 Deviation 
               
               
                   
               
             
          
         
       
     
     
       
         
               
             
               
               
               
             
               
               
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                   
               
               
                 INSTRUMENTAL EVALUATION OF THE HYDRATING EFFECT 
               
               
                 tewametric indexes 
               
               
                 Product: 01.042 
               
               
                 Table 2C.3 
               
             
          
           
               
                 Volunteer&#39;s 
                 tr V 0 co V difference (%) 
                 Reactive 
               
             
          
           
               
                 reference 
                 T1 
                 T2 
                 T3 
                 T4 
                 volunteers* 
               
               
                   
               
             
          
           
               
                 1 
                 −6.3 
                 14.8 
                 69.9 
                 38.6 
                 X 
               
               
                 2 
                 320.0 
                 250.0 
                 250.00 
                 310.00 
                 X 
               
               
                 3 
                 −23.8 
                 1.2 
                 36.9 
                 17.9 
                 X 
               
               
                 4 
                 31.8 
                 45.9 
                 27.2 
                 18.8 
                 X 
               
               
                 5 
                 25.8 
                 38.0 
                 38.7 
                 24.5 
                 X 
               
               
                 6 
                 27.4 
                 44.8 
                 29.4 
                 11.8 
                 X 
               
               
                 7 
                 10.3 
                 65.4 
                 1.9 
                 1.9 
                 X 
               
               
                 8 
                 −17.1 
                 −9.8 
                 −3.9 
                 −13.7 
                 X 
               
               
                 9 
                 12.0 
                 32.0 
                 0.0 
                 0.0 
                 X 
               
               
                 10 
                 25.0 
                 10.0 
                 0.0 
                 50.0 
                 X 
               
               
                   
               
               
                 Key: 
               
               
                 / = no 
               
               
                 X = yes 
               
               
                 *volunteers whose difference between the variation percentage in comparison to T0 of treated and control areas is equal to or above 10% 
               
             
          
         
       
     
     
       
         
               
             
               
               
             
               
               
               
             
               
               
               
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
               
               
               
             
           
               
                 TABLE 2D 
               
             
             
               
                   
               
               
                 INSTRUMENTAL EVALUATION OF THE HYDRATING EFFECT 
               
               
                 tewametric indexes 
               
               
                 Product 01.043 (Formula 4) 
               
               
                 Table 2D.1 
               
             
          
           
               
                   
                 Control Area 
               
             
          
           
               
                   
                   
                 co V % 
               
               
                   
                   
                 Variation percentages in 
               
               
                 Volunteer&#39;s 
                 Absolute Values 
                 comparison with T0 
               
             
          
           
               
                 reference 
                 T0 
                 T1 
                 T2 
                 T3 
                 T4 
                 T1 
                 T2 
                 T3 
                 T4 
               
               
                   
               
             
          
           
               
                 1 
                 1.2 
                 1.2 
                 1.5 
                 1.5 
                 1.7 
                 0.0 
                 28.3 
                 25.0 
                 41.7 
               
               
                 2 
                 2.1 
                 2.0 
                 2.1 
                 2.5 
                 2.0 
                 −4.8 
                 0.0 
                 19.0 
                 −4.8 
               
               
                 3 
                 2.5 
                 2.3 
                 2.3 
                 2.0 
                 1.9 
                 −8.0 
                 −8.0 
                 −20.0 
                 −24.0 
               
               
                 4 
                 4.5 
                 4.6 
                 5.0 
                 5.5 
                 4.8 
                 2.2 
                 11.1 
                 22.2 
                 6.7 
               
               
                 5 
                 2.1 
                 2.6 
                 2.6 
                 2.8 
                 2.6 
                 23.8 
                 23.8 
                 33.3 
                 23.8 
               
               
                 6 
                 5.0 
                 5.1 
                 5.0 
                 5.4 
                 5.3 
                 2.0 
                 0.0 
                 8.0 
                 6.0 
               
               
                 7 
                 2.0 
                 2.1 
                 1.8 
                 2.2 
                 2.0 
                 5.0 
                 −10.0 
                 10.0 
                 0.0 
               
               
                 8 
                 8.0 
                 7.5 
                 7.8 
                 8.7 
                 8.2 
                 −6.3 
                 −2.5 
                 8.7 
                 2.5 
               
               
                 9 
                 4.2 
                 4.0 
                 4.0 
                 3.5 
                 3.8 
                 −4.8 
                 −4.8 
                 −16.7 
                 −9.5 
               
               
                 10  
                 2.8 
                 3.0 
                 3.2 
                 3.6 
                 3.1 
                 7.1 
                 14.3 
                 28.6 
                 10.7 
               
               
                 Vol. No. 
                 10 
                 10 
                 10 
                 10 
                 10 
                 10 
                 10 
                 10 
                 10 
               
               
                 Average 
                 3.4 
                 3.4 
                 3.5 
                 3.8 
                 3.5 
                 1.6% 
                 5.2% 
                 11.8% 
                 5.3% 
               
               
                 Standard 
                 2.0 
                 1.9 
                 2.0 
                 2.2 
                 2.1 
                 9.3% 
                 13.4% 
                 18.0% 
                 18.0% 
               
               
                 Deviation 
               
               
                   
               
             
          
         
       
     
     
       
         
               
               
             
               
               
               
             
               
               
               
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
               
               
               
             
           
               
                   
                 TABLE 2D.2 
               
             
             
               
                   
                   
               
               
                   
                 Treated Area 
               
             
          
           
               
                   
                   
                 tr V % 
               
               
                   
                   
                 Variation percentages in comparison 
               
               
                 Volunteer&#39;s 
                 Absolute Values 
                 with T0 
               
             
          
           
               
                 reference 
                 T0 
                 T1 
                 T2 
                 T3 
                 T4 
                 T1 
                 T2 
                 T3 
                 T4 
               
               
                   
               
             
          
           
               
                 1 
                 0.8 
                 2.5 
                 2.0 
                 2.0 
                 2.0 
                 212.5 
                 150.0 
                 150.0 
                 150.0 
               
               
                 2 
                 0.3 
                 2.1 
                 2.1 
                 2.0 
                 1.5 
                 600.0 
                 600.0 
                 566.7 
                 400.0 
               
               
                 3 
                 0.8 
                 1.0 
                 1.0 
                 1.3 
                 0.8 
                 25.0 
                 25.0 
                 62.5 
                 0.0 
               
               
                 4 
                 2.5 
                 4.0 
                 4.3 
                 4.3 
                 4.3 
                 60.0 
                 72.0 
                 72.0 
                 72.0 
               
               
                 5 
                 5.1 
                 4.1 
                 4.3 
                 4.3 
                 4.0 
                 −19.6 
                 −15.7 
                 −15.7 
                 −21.6 
               
               
                 6 
                 2.0 
                 4.3 
                 5.1 
                 4.5 
                 4.7 
                 115.0 
                 155.0 
                 125.0 
                 135.0 
               
               
                 7 
                 1.4 
                 1.2 
                 1.6 
                 1.3 
                 1.4 
                 −14.3 
                 14.3 
                 −7.1 
                 0.0 
               
               
                 8 
                 13.6 
                 10.0 
                 10.0 
                 9.2 
                 9.2 
                 −26.5 
                 −26.5 
                 −32.4 
                 −32.4 
               
               
                 9 
                 5.9 
                 4.0 
                 3.8 
                 4.0 
                 4.0 
                 −32.2 
                 −35.6 
                 −32.2 
                 −32.2 
               
               
                 10  
                 4.7 
                 4.3 
                 4.2 
                 4.0 
                 4.4 
                 −8.5 
                 −10.6 
                 −14.9 
                 −6.4 
               
               
                 Vol. No. 
                 10 
                 10 
                 10 
                 10 
                 10 
                 10 
                 10 
                 10 
                 10 
               
               
                 Average 
                 3.7 
                 3.8 
                 3.8 
                 3.7 
                 3.6 
                 91.1% 
                 92.8% 
                 87.4% 
                 66.4% 
               
               
                 Standard 
                 4.0 
                 2.5 
                 2.6 
                 2.3 
                 2.4 
                 194.9% 
                 191.1% 
                 180.9% 
                 135.1% 
               
               
                 Deviation 
               
               
                   
               
             
          
         
       
     
     
       
         
               
             
               
               
               
               
             
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
             
           
               
                   
               
               
                 INSTRUMENTAL EVALUATION OF THE HYDRATING EFFECT 
               
               
                 tewametric indexes 
               
               
                 Product: 01.043 
               
               
                 Table 2D.3 
               
             
          
           
               
                   
                 Volunteer&#39;s 
                 tr V 0 co V difference (%) 
                 Reactive 
               
             
          
           
               
                   
                 reference 
                 T1 
                 T2 
                 T3 
                 T4 
                 volunteers* 
               
               
                   
                   
               
             
          
           
               
                   
                 1 
                 212.5 
                 121.7 
                 125.0 
                 108.3 
                 X 
               
               
                   
                 2 
                 604.8 
                 600.0 
                 547.6 
                 404.8 
                 X 
               
               
                   
                 3 
                 33.0 
                 33.0 
                 82.5 
                 24.0 
                 X 
               
               
                   
                 4 
                 57.8 
                 60.9 
                 49.8 
                 65.3 
                 X 
               
               
                   
                 5 
                 −43.4 
                 −39.5 
                 −49.0 
                 −45.4 
                 X 
               
               
                   
                 6 
                 113.0 
                 155.0 
                 117.0 
                 129.0 
                 X 
               
               
                   
                 7 
                 −19.3 
                 24.3 
                 −17.1 
                 0.0 
                 X 
               
               
                   
                 8 
                 −20.2 
                 −24.0 
                 −41.1 
                 −34.9 
                 X 
               
               
                   
                 9 
                 −27.4 
                 −30.8 
                 −15.5 
                 −22.7 
                 X 
               
               
                   
                 10 
                 −15.7 
                 −24.9 
                 043.5 
                 −17.1 
                 X 
               
               
                   
                   
               
               
                   
                 Key: 
               
               
                   
                 / = no 
               
               
                   
                 X = yes 
               
               
                   
                 *volunteers whose difference between the variation percentage in comparison to T0 of treated and control areas is equal to or above 10% 
               
             
          
         
       
     
     
       
         
               
             
               
               
               
               
               
             
               
               
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
               
               
             
           
               
                 TABLE 3 
               
             
             
               
                   
               
               
                 INSTRUMENTAL EVALUATION OF THE HYDRATING EFFECT 
               
               
                 Randomizing Table 
               
             
          
           
               
                 Ref. 
                 01.034 
                 01.041 
                 01.042 
                 01.043 
               
             
          
           
               
                 Vol. 
                 Treated 
                 Control 
                 Treated 
                 Control 
                 Treated 
                 Control 
                 Treated 
                 Control 
               
               
                   
               
             
          
           
               
                 1 
                 R 
                 L 
                 R 
                 L 
                 L 
                 R 
                 L 
                 R 
               
               
                 2 
                 R 
                 L 
                 R 
                 L 
                 L 
                 R 
                 L 
                 R 
               
               
                 3 
                 L 
                 R 
                 L 
                 R 
                 R 
                 L 
                 R 
                 L 
               
               
                 4 
                 R 
                 L 
                 R 
                 L 
                 L 
                 R 
                 L 
                 R 
               
               
                 5 
                 L 
                 R 
                 L 
                 R 
                 R 
                 L 
                 R 
                 L 
               
               
                 6 
                 L 
                 R 
                 L 
                 R 
                 R 
                 L 
                 R 
                 L 
               
               
                 7 
                 R 
                 L 
                 R 
                 L 
                 L 
                 R 
                 L 
                 R 
               
               
                 8 
                 L 
                 R 
                 L 
                 R 
                 R 
                 L 
                 R 
                 L 
               
               
                 9 
                 L 
                 R 
                 L 
                 R 
                 R 
                 L 
                 R 
                 L 
               
               
                 10 
                 L 
                 R 
                 L 
                 R 
                 R 
                 L 
                 R 
                 L 
               
               
                   
               
               
                 Key: D 
               
               
                 R = right forearm 
               
               
                 L = left forearm 
               
             
          
         
       
     
     FORMULAS 
     Formula 1: Reference 01.041 
     
         
         0.2%  Astrocaryum murumuru  Creamy Emulsion 
       
    
     Phase A (%pp) 
     
         
         Non-ionic self-emulsifying base 5.00 
         Keto-stearic alcohol 2.00 
         Lanolin Alcohol, mineral oil 2.00 
         Cetyl Acetate, acetylated lanolin alcohol 2.00 
         Murumuru butter 0.20 
         Butyparabene, Ethylparabene, Methylparabene, Phenoxyethanol, Proplyparabene 0.50 
       
    
     Phase B 
     
         
         Distilled water qsp 100.00 
         Disodium edetate 99.3% 
       
    
     Formula 2: Reference 01.042 
     
         
         1%  Astrocaryum murumuru  Creamy Emulsion 
       
    
     Phase A (%pp) 
     
         
         Non-ionic self-emulsifying base 5.00 
         Keto-stearic alcohol 2.00 
         Lanolin, Alcohol, mineral oil 2.00 
         Cetyl Acetate, acetylated lanoliun alcohol 2.00 
         Murumuru butter 0.50 
         butyparabene, Ethyparabene, Methylparabene, Propylparabene 0.50 
       
    
     Phase B 
     
         
         Distilled water qsp 100.00 
         Dosodium edate 99.3% 
       
    
     Formula 3: Reference 01.034 
     
         
         3%  Astrocaryum murumuru  Creamy Emulsion 
       
    
                                                   (% pp)                                    PHASE A           Non-ionic self-emulsifying base   5.00       Keto-stearic alcohol   2.00       Lanolin, Alcohol, mineral oil   2.00       Cetyl Acetate, acetylated Lanolin alcohol   2.00         Murumuru  butter   3.00       Butylparabene, Ethylparabene, Methylparabene, Phenoxyethanol, Propylparabene   .50       PHASE B       Distilled water qsp   100.00       Disodium edetate 99.3%   0.10       FORMULA 4: Reference 01.043       Creamy Emulsion - Vehicle       PHASE A       Non-ionic self-emulsifying base   5.00       Keto-stearic alcohol   2.00       Lanolin, Alcohol, mineral oil   2.00       Cetyl Acetate, acetylated lanolin alcohol   2.00       Butylparabene, Ethylparabene, Methylparabene, Phenoxyethanol, Propylparabene   .50       PHASE B       Distilled water qsp   100.00       Disodium edetate 99.3%   0.10                    
Equivalents
 
     The present invention encompasses many equivalents not specifically disclosed herein. Such equivalents are intended to be encompassed within the scope of the following claims.