Abstract:
The present invention is a technical filed of pharmaceutical preparation agent, and more particular to a pharmaceutical composition containing docetaxe. The pharmaceutical composition containing docetaxe includes docetaxel and a polyethylene glycol methyl ether-polylactide block copolymer with amino acid end groups. The advantage of the present invention is subjected to a tri-block copolymer with low toxicity as the drug carrier. In addition to the pharmaceutical active ingredients and carrier, there is no other additive in pharmaceutical composition containing docetaxe. Thus, the prescription is simpler and the security is higher. In pharmaceutical component containing docetaxel, docetaxel content is more than 20%, such that the preparation process of pharmaceutical component containing docetaxel is simple, and easy for the industrial applications.

Description:
CROSS-REFERENCE TO RELATED APPLICATIONS 
       [0001]    This application claims the benefit of priority under 35 U.S.C. §119(e) to China Patent application No. 201410336464.x filed on Jul. 15, 2014, China Patent Application No. 201410336638.2 filed on Jul. 15, 2014, China patent application No. 201410336424.5 filed on Jul. 15, 2014, and China Patent Application No. 201410336415.6 filed on Jul. 15, 2014. The aforementioned patent application is incorporated herein by reference in its entirety. 
       FIELD OF THE INVENTION 
       [0002]    The present invention relates to a technical field of pharmaceutical preparation, particularly relates to a pharmaceutical composition containing docetaxel. 
       BACKGROUND OF THE INVENTION 
       [0003]    Docetaxel (docetaxel, Docetaxel, C 43  HS 3 NO 14 ) is based on the structure of a natural anticancer drug taxol, which is a novel anticancer drugs obtained after structural modification. The resistance spectrum of docetaxel and paclitaxel are similar. The mechanism of docetaxel is to promote tubulin polymerization and prevent microtubule depolymerization, thereby the mitosis and proliferation of the cancer cell is to be inhibited, and the efficacy of docetaxel is better than that of paclitaxel. 
         [0004]    In vitro studies show that the cell lines in the human breast, colon, bladder, and epithelial with IC50 is less than 9 times paclitaxel. In vivo studies suggest that docetaxel has a high degree of anti-tumor activity, after treatment and transplanted tumors in mice; murine tumor can be completely dissipated. More importantly, docetaxel to paclitaxel-resistant cell line does not spontaneously produce docetaxel cross-resistance. 
         [0005]    Docetaxel is white or almost white powder which is a drug with highly fat-soluble and insoluble in water. The solubility of docetaxel in water is 6-7 μg/ml. In order to increase the solubility of docetaxel, the currently commercial formulation of docetaxel are required to add Tween-80 as a surfactant. The use of Tween 80 has two major drawbacks. 
         [0006]    The first drawback is that the strong adverse reaction is brought to patients. Tween-80 may cause adverse reaction that includes allergic reactions, hemolysis, cardiovascular adverse reactions and fluid retention. The publication document has been published such as Tween-80 cause allergic reactions animal preliminary exploration, Toxicology 2007, 04, and Pharmacology, pharmacokinetics Research and Analysis Methods 80 pharmaceutical excipients Twain, Chinese medicine thing, 2008, Vol. 22, No. 8. Therefore, the patients need to the oral glucocorticoids drug desensitization in advance. Accordingly, an allergies reaction has been occurred in patients that need to injection of epinephrine, which will undoubtedly increase the burden on patients. 
         [0007]    The second drawback is that the administration method is more complex, more difficult to use. Take Taxotere as an example, the concentration drug is mixed with the dilution to prepare the pre-mixed solution, and then the pre-mixed solution is diluted with 0.9% physiological saline solution to prepare the pre-mixed dilution. Within 4 hours, the pre-mixed dilution is infused for 1 hour. In infusion process, the concentration drug mixed with the dilution requires to upside down carefully about 45 seconds but did not require stirring. The bubbles might be generated in the resulted solution, such that the resulted solution is standing for 5 minutes to discharge the bubbles. The docetaxel injection product, Docetaxel, is produced by Qilu pharmaceutical production. The specification has the following caption: “the labeled amount of each bottle is 1 ml: 20 mg of docetaxel; the actual docetaxel solution is filled with 1.2 ml of 20 mg/ml, equivalent to 24 mg of docetaxel. This volume is used for the supplement for the volume lost due to the viscous liquid is adhered on the bottle wall, and the amount of the liquid cannot be withdrawn from the bottle which is called “dead volume”. During operation, a syringe extracts docetaxel from the bottle and dilutes to 5% glucose injection solution or 0.9% sodium chloride injection solution. In order to avoid the drug overdose toxicity, the vial and syringe cannot be rinsed by solvent 
         [0008]    The polymer micelle with nanoscale particle size is a core-shell structure which is spontaneously formed by amphiphilic block copolymers. The polymer micelle is used as an administration carrier that is firstly proposed by Bader et al in 1984. The amphiphilic block copolymer encapsulated the drug within a hydrophobic core of the polymer micelle to achieve the solubility of insoluble drugs and further improve the bioavailability effect. In addition, good polymeric micelles can prolong the drug circulation time in the human body, reduce the toxicity, and through the EPR effect so as to reach the passive targeting. 
         [0009]    The docetaxel micelle which is made by polymer micelles can overcome the drawbacks of commercially available docetaxel agents. However, the research result for the docetaxel micelle lacks of practical value, the major drawbacks include the particle size is too large to play the EPR effect, lower drug loading, the poor stability of the preparation. For example, in Shandong University, KeWei Yu utilizes Pluronic F68 as a micelle carrier, vitamin ETPGS as a solubilizer to encapsulate the docetaxel, such that the average drug loading of the prepared docetaxel micelle only 0.923%. The average particle diameter of the prepared docetaxel micelle is up to 135.1±3.42 nm (Docetaxel polymeric micelles research by KeWei Yu). In Shandong University, Zhai et al utilize TPGS, MPEG-PLA, CSO-SA as a micelles carrier material to obtain the docetaxel micelles, and the drug loading does not exceed 5.2%, the entitled as “a docetaxel mixed micelles and preparation method contained, China patent application No. 201210372072.X). 
         [0010]    The freeze-dried powder is an effective way to deposit the drug. The product is dried at low temperatures so that the moisture contained in the product is to be frozen, and then the product is placed in a vacuum environment to be dried, so as to the water is directly sublimed from a solid state to vapor and is excluded from the product, such that the product activity is dried. This method effectively prevents the change of the physicochemical and biological properties of the product, and effectively to protect the stability of the heat-sensitive pharmaceutical active ingredient. The freeze-dried powder in the form of loose after drying, and the colors of the freeze-dried powder basically does not change. The freeze-dried powder can quickly dissolve with water or a hydrophilic organic solvent and restore the physical and chemical properties and biological activity for the original aqueous solution. Because the freeze-dried powder is dried under vacuum conditions, for some easily oxidized substance has a good protective effect. The moisture content of freeze-dried powder product after lyophilization procedure is very low, so that the stability of freeze-dried powder product is increased, and the chance of contamination is to be decreased. Therefore, the preparation method is not only convenient transportation also extended the shelf life of the product. 
         [0011]    At present, the research result for the docetaxel freeze-dried powder did not achieve a breakthrough, especially in the components, the particle size after reconstitution and the amount of drug loading and so on. ShouZhu Hao published a method for preparing docetaxel lyophilized powder (the entitled as “A docetaxel pharmaceutical composition, preparation method and use”, China Patent application No. 200780000695.1), and the disclosure of ShouZhu Hao still contains a freeze-dried powder spit temperature 80. Shandong University, Zhang Na et al utilize PLA-PEG copolymer micelles to obtain the docetaxel freeze-dried powder, but the particle size of the docetaxel freeze-dried powder after reconstitution is greater than 200 nm or more (The entitled as “preparation contained docetaxel nanoparticles mixed micelles and the freeze-drying agent”, China Patent application No. 201010151501.1). 
       SUMMARY OF THE INVENTION 
       [0012]    The objective of the present invention is to overcome the drawbacks of the prior art. The present invention provides a pharmaceutical compositions containing docetaxel. The pharmaceutical composition of the present invention is manufactured by polyethylene glycol methyl ether-polylactide block copolymer with amino end groups as the carrier material and docetaxel. The prescription of pharmaceutical composition is simple, safe and reliable. The amount of drug loading for docetaxel in pharmaceutical composition can exceed 25%. The particle size of the freeze-dried powder is small after reconstitution small and having high stability. 
         [0013]    The technical solutions of the present invention are described as follows. 
         [0014]    The pharmaceutical composition containing docetaxel is manufactured by polyethylene glycol methyl ether-polylactide block copolymer with amino acid end groups and docetaxel, in which the chemical structure of polyethylene glycol methyl ether-polylactide block copolymer with amino end groups as follows 
         [0000]    
       
                 
         
             
             
         
       
     
         [0015]    In the chemical formula (I), the functional group R is an amino acid group, the subscript a is ranging from 10-200, the subscript b is ranging from 3-30, the average molecular weight of polyethylene glycol methyl ether is ranging from 1,000-3500, the average molecular weight of polylactide block copolymer is ranging from 450-5500, the component of docetaxel is ranging from 3.21-27.75 by weight, and the component of polyethylene glycol methyl ether-polylactide block copolymer with amino acid end groups is 100 by weight. 
         [0016]    In the above formula (I), the functional group R is amino acid group and chemical structure is 
         [0000]    
       
                 
         
             
             
         
       
     
         [0000]    in which the functional group R 1  is H, CH 3 , (CH 3 ) 2  CH, PhCH 2  or (CH 2 ) 3  NHC(NH)NH 2 . 
         [0017]    Polyethylene glycol methyl ether of polyethylene glycol methyl ether-polylactide block copolymer with amino acid end groups that having higher stability and is less prone to be degradated. Polylactide block copolymer of polyethylene glycol methyl ether-polylactide block copolymer with amino acid end groups is degradated only under strong acid conditions. The graft amino acid is reacted under neutral conditions. Therefore, the stability of polyethylene glycol methyl ether-polylactide block copolymer with amino acid end groups is higher under normal solution environment. The average molecular weight of the different tri-blocks for polyethylene glycol methyl ether and polylactide block copolymer of polyethylene glycol methyl ether-polylactide block copolymer with amino acid end groups is obtained by mass spectrometry analysis. 
         [0018]    The aforementioned pharmaceutical composition containing docetaxel is a freeze-dried powder, which is manufactured by lyophilization method. 
         [0019]    The docetaxel of the present invention is anhydrous docetaxel, and the purity of docetaxel is counted according to chemical formula, C 43 H 53 NO 14 , the purity of docetaxel is more than 98.0%. 
         [0020]    The study of the present invention shows that polyethylene glycol methyl ether-polylactide block copolymer with amino acid end groups having no significant carcinogenic, no reproductive toxicity, teratogenic, or mutagenic. The polyethylene glycol methyl ether-polylactide block copolymer with amino acid end groups is to be degradated as latic acid or amino PEG in human body and can be directly discharged from the human body. The present invention is subjected to acute toxicity tests within mice, LD50&gt;2.00 g/kg in mice. During the long-term toxicity test, the dose was 1.00 g/kg, 1 time/day, continuing administrated for 2 days a week. Then, stopping administrated 5 days. Next, continuing administrated for 13 weeks, and restoring for 4 weeks for observation. The results show that there are no significant side effects in mice. 
         [0021]    By cytotoxicity tests show that cytotoxicity of tri-block copolymers is lower than that of mPEG-PLA block copolymer, and mPEG-PLA block copolymer is well-known non-toxicity copolymer currently, in which Table 1 to Table 4 respectively shows the results for polyethylene glycol methyl ether-polylactide block copolymer with different amino acid end groups by using MTT assay hepatotoxicity test. 
         [0000]    
       
         
               
             
               
               
               
             
               
               
               
               
               
             
               
               
               
               
               
             
           
               
                 TABLE 1 
               
             
             
               
                   
               
               
                 Hepatocyte toxicity test results by MTT method: Polyethylene 
               
               
                 glycol methyl ether-polylactide-lysine 
               
             
          
           
               
                   
                 Polyethylene 
                   
               
               
                   
                 glycol methyl 
               
               
                 Concentration 
                 ether-polylactide-lysine 
                 mPEG-PLA 
               
             
          
           
               
                 μg/mL 
                 Cell viability % 
                 error % 
                 Cell viability % 
                 error % 
               
               
                   
               
             
          
           
               
                 0 
                 100 
                 ±3.23 
                 96 
                 ±4.22 
               
               
                 10 
                 99.72 
                 ±6.53 
                 91.24 
                 ±3.25 
               
               
                 100 
                 98.95 
                 ±2.32 
                 93.41 
                 ±4.77 
               
               
                 250 
                 97.23 
                 ±3.51 
                 90.07 
                 ±3.34 
               
               
                 500 
                 97.16 
                 ±2.53 
                 85.45 
                 ±5.72 
               
               
                 1000 
                 96.31 
                 ±2.34 
                 76.28 
                 ±5.28 
               
               
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
             
               
               
               
               
               
             
               
               
               
               
               
             
           
               
                 TABLE 2 
               
             
             
               
                   
               
               
                 Hepatocyte toxicity test results by MTT method: Polyethylene 
               
               
                 glycol methyl ether-polylactide-aspartate 
               
             
          
           
               
                   
                 Polyethylene glycol methyl 
                   
               
               
                 Concentration 
                 ether-polylactide-aspartate 
                 mPEG-PLA 
               
             
          
           
               
                 μg/mL 
                 Cell viability % 
                 error % 
                 Cell viability % 
                 error % 
               
               
                   
               
             
          
           
               
                 0 
                 100 
                 ±3.23 
                 96 
                 ±4.22 
               
               
                 10 
                 99.72 
                 ±6.53 
                 91.24 
                 ±3.25 
               
               
                 100 
                 98.95 
                 ±2.32 
                 93.41 
                 ±4.77 
               
               
                 250 
                 97.23 
                 ±3.51 
                 90.07 
                 ±3.34 
               
               
                 500 
                 97.16 
                 ±2.53 
                 85.45 
                 ±5.72 
               
               
                 1000 
                 96.31 
                 ±2.34 
                 76.28 
                 ±5.28 
               
               
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
             
               
               
               
               
               
             
               
               
               
               
               
             
           
               
                 TABLE 3 
               
             
             
               
                   
               
               
                 Hepatocyte toxicity test results by MTT method: Polyethylene 
               
               
                 glycol methyl ether-polylactide-glutamic acid 
               
             
          
           
               
                   
                 Polyethylene 
                   
               
               
                   
                 glycol methyl ether- 
               
               
                 Concentration 
                 polylactide-glutamic acid 
                 mPEG-PLA 
               
             
          
           
               
                 μg/mL 
                 Cell viability % 
                 error % 
                 Cell viability % 
                 error % 
               
               
                   
               
             
          
           
               
                 0 
                 100 
                 ±3.23 
                 96 
                 ±4.22 
               
               
                 10 
                 99.72 
                 ±6.53 
                 91.24 
                 ±3.25 
               
               
                 100 
                 98.95 
                 ±2.32 
                 93.41 
                 ±4.77 
               
               
                 250 
                 97.23 
                 ±3.51 
                 90.07 
                 ±3.34 
               
               
                 500 
                 97.16 
                 ±2.53 
                 85.45 
                 ±5.72 
               
               
                 1000 
                 96.31 
                 ±2.34 
                 76.28 
                 ±5.28 
               
               
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
             
               
               
               
               
               
             
               
               
               
               
               
             
           
               
                 TABLE 4 
               
             
             
               
                   
               
               
                 Hepatocyte toxicity test results by MTT method: Polyethylene 
               
               
                 glycol methyl ether-polylactide-phenylalanine 
               
             
          
           
               
                   
                 Polyethylene 
                   
               
               
                   
                 glycol methyl ether- 
               
               
                 Concentration 
                 polylactide-phenylalanine 
                 mPEG-PLA 
               
             
          
           
               
                 μg/mL 
                 Cell viability % 
                 error % 
                 Cell viability % 
                 error % 
               
               
                   
               
             
          
           
               
                 0 
                 100 
                 ±3.23 
                 96 
                 ±4.22 
               
               
                 10 
                 99.72 
                 ±6.53 
                 91.24 
                 ±3.25 
               
               
                 100 
                 98.95 
                 ±2.32 
                 93.41 
                 ±4.77 
               
               
                 250 
                 97.23 
                 ±3.51 
                 90.07 
                 ±3.34 
               
               
                 500 
                 97.16 
                 ±2.53 
                 85.45 
                 ±5.72 
               
               
                 1000 
                 96.31 
                 ±2.34 
                 76.28 
                 ±5.28 
               
               
                   
               
             
          
         
       
     
         [0022]    To compared with the prior art, the advantageous effects of present invention as follows: 
         [0023]    1. The present invention is subjected to polyethylene glycol methyl ether-polylactide block copolymer with amino acid end groups with low toxicity as the drug carrier, in addition to the pharmaceutical active ingredients and carrier, there is no other additives in polyethylene glycol methyl ether-polylactide block copolymer with amino acid end groups. Thus, the prescription is simpler and the security is higher. 
         [0024]    2. The composition may be manufactured by lyophilization process for the pharmaceutical industry to form the solid freeze-dried powder. The solid freeze-dried powder can be quickly redissolved in the physiological saline, water for injection or glucose for injection, so that the preparation, transport, storage and use for the drug are more convenient. 
         [0025]    3. In pharmaceutical component containing docetaxel, docetaxel content is more than 20%, such that the preparation process of pharmaceutical component containing docetaxel is simple, and easy for the industrial applications. 
     
    
     DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENT 
       [0026]    Some embodiments of the invention will now be described in greater detail. Nevertheless, it should be recognized that the present invention can be practiced in a wide range of other embodiments besides those explicitly described, and the scope of the present invention is expressly not limited except as specified in the accompanying claims. The following materials, reagents, and like are used in the following embodiment, if no special instructions, the material, reagents can be obtained from the commercial way. 
         [0027]    In the present invention, the formula of polyethylene glycol methyl ether-polylactide block copolymer with amino acid end groups as follows: 
         [0000]    
       
                 
         
             
             
         
       
     
         [0028]    In the chemical formula (I), the functional group R is an amino acid, the subscript a is ranging from 10-200, the subscript b is ranging from 3-30, the average molecular weight of polyethylene glycol methyl ether block is ranging from 1000-3500, and the average molecular weight of polylactide block is ranging from 450-5500. 
         [0029]    In formula (I), the functional group R is amino group and the chemical structure is 
         [0000]    
       
                 
         
             
             
         
       
     
         [0000]    wherein the functional group R 1  is H, CH 3 (CH 3 ) 2 CH, PhCH 2  or (CH 2 ) 3 NHC(NH)NH 2 . 
       Examples 1-7 
       [0030]    Docetaxel is anhydrous docetaxel (CAS: 114977-28-5), which is produced by Xi&#39;an Tianfeng natural field Bio-Technique Co., Ltd. 
         [0031]    In Examples 1-7, polyethylene glycol methyl ether-polylactide block copolymer with amino acid end groups is polyethylene glycol methyl ether-polylactide-lysine, and the chemical formula of polyethylene glycol methyl ether-polylactide-lysine as follows: 
         [0000]    
       
                 
         
             
             
         
       
     
         [0000]    wherein the subscript a in formula (II) is ranging from 10-200, the subscript b in formula (II) is ranging from 3-30. For polyethylene glycol methyl ether-polylactide-lysine, the average molecular weight of polylactide block is ranging from 505-4982 and the average molecular weight of polyethylene glycol methyl ether block is ranging from 1017-2992. Polyethylene glycol methyl ether-polylactide-lysine is prepared according to China patent application No. 2013000453. The average molecular weight of different blocks is determined by mass spectrum analysis with molecular weight of the materials. 
         [0032]    In Examples 1-7, pharmaceutical component of pharmaceutical component containing docetaxel is micelles, which is prepared by film hydration method. The steps of method include: 
         [0000]    (1) Docetaxel and polyethylene glycol methyl ether-polylactide-lysine are weighted respectively according to the different feeding ratios (see Table 5).
 
(2) The above raw materials (which include docetaxel and polyethylene glycol methyl ether-polylactide-lysine) are disposed into a container, and an organic solvent is added into container until the raw materials dissolved completely, in which the organic solvent includes ethanol, acetonitrile or the like. The container is rotated and evaporated under 30-50° C. for 2 hours until the organic solvent was evaporated to dryness. The remaining organic solvent is dried in vacuo at 10-60° C. and is performed more than 12 hours, so as to the polymeric mixed film of docetaxel-containing can be obtained.
 
(3) The polymer mixed film is disposed in a water bath with a temperature at 40-60° C. until the polymer mixed film becomes the transparent. Then, the preheated ultrapure water, physiological saline, or phosphate buffer with same temperature as the polymer mixed film is added into the polymer mixed film (disposed in water bath) and is shaken and hydrated well. Thus, the transparent drug loading micellar solution is obtained.
 
(4) The drug loading micelle solution is filtered with 0.45 μm filter membrane to obtain micelles.
 
(5) The drug loading micelle is subjected to the lyophilization process without adding any excipient to obtain the solid freeze-dried powder.
 
         [0033]    The preparation method of the freeze-dried powder includes: 
         [0000]    Step a: a shelf is performed pre-cooling process to 0° C. and the docetaxel micelles is disposed on the shelf to cool to below −45° C., so as to the solution temperature with docetaxel micelles is dropped to −45° C., and the solution is maintained at a temperature −45° C. for 2-4 hours.
 
Step b: the vacuum pump is turned on and vacuumed for 1-1.5 hours after the step a.
 
Step c: the temperature of the spacer plate is set from −45° C. to −25° C. and the temperature of the spacer plate is maintained more than 12 hours. The ultimate vacuum is set at 0.013 Bar.
 
Step d: the temperature of the spacer plate is set from −25° C. to 0° C. and the temperature of the spacer plate is maintained more than 8 hours. The ultimate vacuum is set at 0.013 Bar.
 
Step e: the temperature of the spacer plate is set from 0° C. to 5° C. and the temperature of the spacer plate is maintained more than 3 hours. The ultimate vacuum is set at 0.013 Bar.
 
Step f: the temperature of the spacer plate is set from 5° C. to 20° C. After the temperature of the reached at 20° C., the temperature of the product is maintained more than 4 hours. Then, the lyophilization process is finished and the freeze-dried powder with loose block can be obtained.
 
         [0034]    In addition, according to above steps, an additional step (6) to add an excipient to prepare a freeze-dried powder, and the steps as follows: 
         [0035]    The excipient is added into the docetaxel micelle and the total weight of the excipient is not exceeding 5% of above step (1). The excipient and the docetaxel micelle are fully dissolved. Next, according to above step (5), the lyophilization process is performed to the mixture of the excipient and the docetaxel micelles to prepare the docetaxel freeze-dried powder. The excipient is selected from the group consisting of one or more of lactose, mannitol, dextran, glycine, and glucose. 
         [0036]    In above preparation, the organic solvent is ethanol or acetonitrile in above step (2). 
         [0037]    The role of excipients in the lyophilization process is to promote the solution which is difficult to be solidified, so as to the solid powder can be obtained. The various excipients can also improve the performance indicators such as appearance, shape, solubility, and stability of the docetaxel freeze-dried powder according to varying degree. 
         [0038]    Examples 1-7 select the different feed ratios and the polyethylene glycol methyl ether-polylactide-lysine with different molecular weight, which is prepared in accordance with the above procedure to obtain the docetaxel freeze-dried powder. The amount of the drug loading is determined by HPLC and the average particle size of the solution which is redissolved is determined by dynamic light scattering, see Table 5. 
         [0000]    
       
         
               
             
               
               
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 5 
               
             
             
               
                   
               
               
                 the measurement result for amount of the drug loading and particle size in 
               
               
                 different examples. 
               
             
          
           
               
                   
                 Polyethylene 
                   
                   
                   
                 Average 
               
               
                   
                 glycol methyl 
                   
                   
                   
                 particele 
               
               
                   
                 ether 
                 Polylactide 
                 Feed ration 
                 Acutal drug loading 
                 size 
               
               
                 Example 
                 M.W. 
                 M.W. 
                 Docetaxel:carrier 
                 (Docetaxel:carrier) 
                 (nm) 
               
               
                   
               
             
          
           
               
                 Example 1 
                 1989 
                 843 
                  4:100 
                 3.24:100 
                 20.0 
               
               
                 Example 2 
                 2010 
                 1406 
                  8:100 
                 6.85:100 
                 29.2 
               
               
                 Example 3 
                 1998 
                 1688 
                 16:100 
                 14.28:100  
                 24.9 
               
               
                 Example 4 
                 1973 
                 1923 
                 30:100 
                 27.75:100  
                 26.1 
               
               
                 Example 5 
                 2033 
                 3220 
                 20:100 
                 18.52:100  
                 24.0 
               
               
                 Example 6 
                 1017 
                 505 
                 10:100 
                 8.90:100 
                 26.9 
               
               
                 Example 7 
                 2992 
                 4982 
                  6:100 
                 5.26:100 
                 26.5 
               
               
                   
               
               
                 Carrier: polyethylene glycol methyl ether-polylactide-lysine 
               
             
          
         
       
     
         [0039]    In accordance with the ratio of 3 mg/ml concentration of docetaxel, the docetaxel freeze-dried powder and water are weighted respectively which is prepared by Examples 1-7. The above freeze-dried powder is fed into the water for injection, the physiological saline or glucose for injection. Next, the docetaxel freeze-dried powder is fully dissolved after shaking with 60 seconds, so as to the docetaxel freeze-dried powder indicates good solubility. The average particle size distribution of the solution (or called redissolved solution) with docetaxel freeze-dried powder is determined, and the particle size of the redissolved solution is ranging from 10-100 nm, an average particle size is ranging from 20-29.2 nm, as shown in Table 6. The redissolved solution is respectively observered at 15° C., 25° C., 30′C and under the normal indoor lighting condition every 2 hours, until the solution appears cloudy or the precipitation was found. The observation result shows that the end of the steady state of the redissolved solution. The results are shown in Table 6, which illustrate the docetaxel freeze-dried powder of the present invention has good stability. 
         [0000]    
       
         
               
             
               
               
               
               
             
               
               
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 6 
               
             
             
               
                   
               
               
                 the test results for the average particle size and the 
               
               
                 stability for the redissolved soltion in different Examples 
               
             
          
           
               
                   
                   
                 Average 
                   
               
               
                   
                 Redissolved 
                 particle 
                 Settling time (Hour) 
               
             
          
           
               
                 Example 
                 solvent 
                 size (nm) 
                 15° C. 
                 25° C. 
                 30° C. 
               
               
                   
               
             
          
           
               
                 Example1 
                 Water for 
                 20.0 
                 26 
                 14 
                 6 
               
               
                   
                 injection 
               
               
                 Example 2 
                 0.9% NaCl 
                 29.2 
                 32 
                 24 
                 14 
               
               
                 Example 3 
                 5.0% Glucose 
                 24.9 
                 28 
                 14 
                 8 
               
               
                 Example 4 
                 0.9% Nacl 
                 26.1 
                 28 
                 12 
                 6 
               
               
                 Example 5 
                 Water for 
                 24.0 
                 24 
                 12 
                 6 
               
               
                   
                 injection 
               
               
                 Example 6 
                 Water for 
                 26.9 
                 18 
                 12 
                 10 
               
               
                   
                 injection 
               
               
                 Example 7 
                 Water for 
                 26.5 
                 16 
                 16 
                 8 
               
               
                   
                 injection 
               
               
                   
               
             
          
         
       
     
         [0040]    Stability Test: 
         [0041]    The docetaxel micelles which is prepared from Examples 1-7 is diluted with water to obtain docetaxel concentration of about 3 mg/ml. The solution (docetaxel micelles diluted with water) is respectively observered at 15° C., 25° C., 30° C. and under the normal indoor lighting condition every 2 hours, until the solution appears cloudy or the precipitation was found. The observation result shows that the end of the steady state of the solution (docetaxel micelles diluted with water). The results are shown in Table 7. 
         [0000]    
       
         
               
             
               
               
             
               
               
               
               
               
             
               
               
               
               
               
             
           
               
                 TABLE 7 
               
             
             
               
                   
               
               
                 the stability test results in different Examples 
               
             
          
           
               
                   
                 Settling time (Hour) 
               
             
          
           
               
                   
                 Example 
                 15° C. 
                 25° C. 
                 30° C. 
               
               
                   
                   
               
             
          
           
               
                   
                 Example 1 
                 26 
                 12 
                 6 
               
               
                   
                 Example 2 
                 32 
                 22 
                 14 
               
               
                   
                 Example 3 
                 28 
                 16 
                 8 
               
               
                   
                 Example 4 
                 28 
                 14 
                 6 
               
               
                   
                 Example 5 
                 24 
                 12 
                 6 
               
               
                   
                 Example 6 
                 18 
                 14 
                 10 
               
               
                   
                 Example 7 
                 16 
                 16 
                 8 
               
               
                   
                   
               
             
          
         
       
     
       Example 8 
       [0042]    The physiological saline, the commercial docetaxel for injection (Taxotere) and the docetaxel micelle solution (prepared by the invention) are subjected to a tumor suppression test. The testing object is Balb/c mice with transplanted L7912 tumor strain. The administration route is intravenous administration, one time every three days, and administered continuously for 30 days. The drug concentration of Taxotere is same as docetaxel of 10 mg/kg of the present invention. The tumor volume in mice was measured twice every week, and the results as shown in Table 8. The results show that the tumor volume in mice with the saline drip is rapidly grown. The growing speed of the tumor volume in mice with the Taxotere drip can be controlled, but the tumor volume is still growing. The tumor volume in mice with the docetaxel of the invention is controlled and reduced quickly, as shown in Table 8. Accordingly, the docetaxel of the invention for the T cell leukemia tumor in mice has good inhibitory effect. 
         [0000]    
       
         
               
             
               
               
               
               
             
               
               
               
               
               
               
             
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
             
           
               
                 TABLE 8 
               
             
             
               
                   
               
               
                 the measurements result for the tumor volume in mice 
               
             
          
           
               
                   
                 Physiological 
                   
                 Docetaxel 
               
               
                   
                 saline 
                 Commerical docetaxel 
                 of the invention 
               
             
          
           
               
                   
                 Tumor 
                   
                 for injection 
                 Tumor 
                   
               
             
          
           
               
                 Time 
                 volume 
                   
                 Tumor volume 
                   
                 volume 
                   
               
               
                 (day) 
                 (Mm 3 ) 
                 variance 
                 (Mm 3 ) 
                 variance 
                 (Mm 3 ) 
                 variance 
               
               
                   
               
             
          
           
               
                 1 
                 165.7 
                 38.0 
                 156.0 
                 13.0 
                 160.0 
                 31.0 
               
               
                 5 
                 246.0 
                 79.0 
                 177.0 
                 7.5 
                 130.8 
                 30.0 
               
               
                 8 
                 307.0 
                 88.0 
                 154.0 
                 8.3 
                 58.0 
                 19.0 
               
               
                 12 
                 401.0 
                 149.0 
                 157.0 
                 4.3 
                 16.1 
                 4.8 
               
               
                 15 
                 466.0 
                 124.0 
                 175.0 
                 3.8 
                 8.8 
                 1.9 
               
               
                 19 
                 552.0 
                 90.0 
                 178.0 
                 7.3 
                 3.2 
                 1.9 
               
               
                 22 
                 642.0 
                 79.6 
                 187.9 
                 13.9 
                 1.8 
                 1.1 
               
               
                 26 
                 707.9 
                 44.9 
                 226.7 
                 27.5 
                 1.6 
                 1.4 
               
               
                 30 
                 861.3 
                 110.7 
                 305.2 
                 52.0 
                 2.6 
                 1.8 
               
               
                   
               
             
          
         
       
     
       Examples 9-15 
       [0043]    In Examples 9-15, Docetaxel is anhydrous docetaxel (CAS: 114977-28-5), which is produced by Xi&#39;an Tianfeng natural field Bio-Technique Co., Ltd. 
         [0044]    In Examples 9-15, polyethylene glycol methyl ether-polylactide block copolymer with amino acid end groups is polyethylene glycol methyl ether-polylactide-aspartic acid, and the chemical formula of polyethylene glycol methyl ether-polylactide-aspartic acid as follows: 
         [0000]    
       
                 
         
             
             
         
       
     
         [0000]    wherein the subscript a in formula (III) is ranging from 10-200, the subscript b in formula (III) is ranging from 3-30. For the polyethylene glycol methyl ether-polylactide-aspartic acid, the average molecular weight of polylactide block is ranging from 495-4996 and the average molecular weight of polyethylene-glycol methyl ether block is ranging from 987-3020. Polyethylene glycol methyl ether-polylactide-aspartic acid is prepared according to China patent application No. 2013000453. The average molecular weight of the different blocks is determined by mass spectrum analysis with molecular weight of the materials. 
         [0045]    In Examples 9-15, the pharmaceutical component of pharmaceutical component containing docetaxel is micelles, which is prepared by film hydration method. The steps of the method include:
   (1) Docetaxel and polyethylene glycol methyl ether-polylactide-aspartic acid are weighted respectively according to different feed ratios (see Table 7).   (2) The above raw materials (which include docetaxel and polyethylene glycol methyl ether-polylactide-aspartic acid) are placed into a container, and an organic solvent is added into container until the raw materials dissolved completely, in which the organic solvent includes ethanol, acetonitrile or the like. The container is rotated and evaporated under 30-50° C. for 2 hours until the organic solvent was evaporated to dryness. The remaining organic solvent is dried in vacuo at 10-60° C. and is performed more than 12 hours, so as to the polymeric mixed film of docetaxel-containing can be obtained.   (3) The polymer mixed film is disposed in a water bath with a temperature at 40-60° until the polymer mixed film becomes the transparent. Then, pre-heated ultrapure water, physiological saline, or phosphate buffer with same temperature as that of the polymer mixed film is added into the polymer mixed film (disposed in water bath) and is shaken and hydrated well. Thus, the transparent drug loading micelle solution is obtained.   (4) The drug loading micelle solution is filtered with 0.45 μm filter membrane to obtain micelles.   (5) The drug loading micelle is subjected to lyophilization process without adding any excipient to obtain the solid freeze-dried powder.   
 
         [0051]    The step of preparation method of the freeze-dried powder includes: 
         [0000]    Step a: a shelf is performed pre-cooling process to 0° C. and the docetaxel micelles is disposed on the shelf to cool to below −45° C., so as to the solution temperature with docetaxel micelles is dropped to −45° C., and the solution is maintained at a temperature −45° C. for 2-4 hours.
 
Step b: the vacuum pump is turned on and vacuumed for 1-1.5 hours after the step a.
 
Step c: the temperature of the spacer plate is set from −45° C. to −25° C. and the temperature of the spacer plate is maintained more than 12 hours. The ultimate vacuum is set at 0.013 Bar.
 
Step d: the temperature of the spacer plate is set from −25° C. to 0° C. and the temperature of the spacer plate is maintained more than 8 hours. The ultimate vacuum is set at 0.013 Bar.
 
Step e: the temperature of the spacer plate is set from 0° C. to 5° C. and the temperature of the spacer plate is maintained more than 3 hours. The ultimate vacuum is set at 0.013 Bar.
 
Step f: the temperature of the spacer plate is set from 5° C. to 20° C. After the temperature of the reached at 20° C., the temperature of the product is maintained more than 4 hours. Then, the lyophilization process is finished and the freeze-dried powder with loose block can be obtained.
 
         [0052]    In addition, according to above steps, an additional step (6) to add an excipient to prepare a freeze-dried powder includes: 
         [0053]    The excipient is added into the docetaxel micelle and the total weight of the excipient is not exceeding 5% of above step (1), and the excipient and the docetaxel micelle are fully dissolved. Next, according to above step (5), the lyophilization process is performed to the mixture of the excipient and the docetaxel micelle to prepare the docetaxel freeze-dried powder. The excipient is selected from the group consisting of one or more of lactose, mannitol, dextran, glycine, and glucose. 
         [0054]    In above preparation, the organic solvent is ethanol or acetonitrile in above step (2). 
         [0055]    The role of excipients in the lyophilization process is to promote the solution which is difficult to be solidified, so as to the solid powder can be obtained. The various excipients can also improve the performance indicators such as appearance, shape, solubility, and stability of the docetaxel freeze-dried powder according to varying degree. 
         [0056]    Examples 9-15 select the different feed ratios and the polyethylene glycol methyl ether-polylactide-aspartic acid with different molecular weight, which is prepared in accordance with the above procedure to obtain the docetaxel freeze-dried powder. The amount of the drug loading is determined by HPLC and the average particle size of the solution which is redissolved is determined by dynamic light scattering, as shown in Table 9. 
         [0000]    
       
         
               
             
               
               
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 9 
               
             
             
               
                   
               
               
                 the measurement result for amount of the drug loading and particle size in 
               
               
                 different examples. 
               
             
          
           
               
                   
                 Polyethylene 
                   
                   
                   
                 Average 
               
               
                   
                 glycol 
                   
                   
                   
                 particele 
               
               
                   
                 methyl ether 
                 Polylactide 
                 Feed ratio 
                 Acutal drug loading 
                 size 
               
               
                 Example 
                 M.W. 
                 M.W. 
                 Docetaxel:carrier 
                 (Docetaxel:carrier) 
                 (nm) 
               
               
                   
               
             
          
           
               
                 Example 9 
                 2018 
                 855 
                  4:100 
                 3.21:100 
                 20.6 
               
               
                 Example 10 
                 2020 
                 1438 
                  8:100 
                 6.22:100 
                 28.1 
               
               
                 Example 11 
                 1981 
                 1660 
                 16:100 
                 13.98:100  
                 25.5 
               
               
                 Example 12 
                 2033 
                 1968 
                 30:100 
                 26.48:100  
                 26.8 
               
               
                 Example 13 
                 1988 
                 3211 
                 20:100 
                 17.87:100  
                 25.3 
               
               
                 Example 14 
                 987 
                 495 
                 10:100 
                 8.56:100 
                 28.1 
               
               
                 Example 15 
                 3020 
                 4996 
                  6:100 
                 4.67:100 
                 26.2 
               
               
                   
               
               
                 Carrier = polyethylene glycol methyl ether-polylactide-aspartate acid 
               
             
          
         
       
     
         [0057]    In accordance with the ratio of 3 mg/ml concentration of docetaxel, the docetaxel freeze-dried powder and water are weighted respectively which is prepared by Examples 9-15. The above freeze-dried powder is fed into the water for injection, the physiological saline or glucose for injection. Next, the docetaxel freeze-dried powder is fully dissolved after shaking about 60 seconds. The docetaxel freeze-dried powder indicates good solubility. The average particle size distribution of the solution (or called redissolved solution) with docetaxel freeze-dried powder is determined, and the particle size of the redissolved solution is ranging from 10-100 nm, an average particle size is ranging from 20-29.2 nm, as shown in Table 10. The redissolved solution is respectively observered at 15° C., 25° C., 30° C. and under the normal indoor lighting condition every 2 hours, until the solution appears cloudy or the precipitation was found. The observation result shows that the end of the steady state of the redissolved solution. The results are shown in Table 10, which illustrate the docetaxel freeze-dried powder of the present invention has good stability. 
         [0000]    
       
         
               
             
               
               
               
               
             
               
               
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 10 
               
             
             
               
                   
               
               
                 the test results for the average particle size and the stability 
               
               
                 for the redissolved soltion in different Examples 
               
             
          
           
               
                   
                   
                 Average 
                   
               
               
                   
                 Redissolved 
                 particle size 
                 Settling time (Hour) 
               
             
          
           
               
                 Example 
                 solvent 
                 (nm) 
                 15° C. 
                 25° C. 
                 30° C. 
               
               
                   
               
             
          
           
               
                 Example 9 
                 Water for 
                 20.6 
                 26 
                 14 
                 6 
               
               
                   
                 injection 
               
               
                 Example 10 
                 0.9% NaCl 
                 28.1 
                 32 
                 24 
                 14 
               
               
                 Example 11 
                 5.0% 
                 25.5 
                 28 
                 14 
                 8 
               
               
                   
                 Glucose 
               
               
                 Example 12 
                 0.9% NaCl 
                 26.8 
                 28 
                 12 
                 6 
               
               
                 Example 13 
                 Water for 
                 25.3 
                 24 
                 12 
                 6 
               
               
                   
                 injection 
               
               
                 Example 14 
                 Water for 
                 28.1 
                 18 
                 12 
                 10 
               
               
                   
                 injection 
               
               
                 Example 15 
                 Water for 
                 26.2 
                 16 
                 16 
                 8 
               
               
                   
                 injection 
               
               
                   
               
             
          
         
       
     
         [0058]    Stability Test 
         [0059]    The docetaxel micelles which is prepared from Examples 9-15 is diluted with water to obtain docetaxel concentration of about 3 mg/ml. The solution (docetaxel micelles diluted with water) is respectively observered at 15° C., 25° C., 30° C. and under the normal indoor lighting condition every 2 hours, until the solution appears cloudy or the precipitation was found. The observation result shows that the end of the steady state of the solution (docetaxel micelles diluted with water). The results are shown in Table 11. 
         [0000]    
       
         
               
             
               
               
             
               
               
               
               
               
             
               
               
               
               
               
             
           
               
                 TABLE 11 
               
             
             
               
                   
               
               
                 the stability test results in different Examples 
               
             
          
           
               
                   
                 Settling time (Hour) 
               
             
          
           
               
                   
                 Example 
                 15° C. 
                 25° C. 
                 30° C. 
               
               
                   
                   
               
             
          
           
               
                   
                 Example 9 
                 26 
                 12 
                 8 
               
               
                   
                 Example 10 
                 32 
                 20 
                 14 
               
               
                   
                 Example 11 
                 28 
                 14 
                 8 
               
               
                   
                 Example 12 
                 28 
                 14 
                 6 
               
               
                   
                 Example 13 
                 24 
                 12 
                 6 
               
               
                   
                 Example 14 
                 18 
                 14 
                 10 
               
               
                   
                 Example 15 
                 16 
                 16 
                 8 
               
               
                   
                   
               
             
          
         
       
     
       Example 16 
       [0060]    The physiological saline, the commercial docetaxel for injection (Taxotere) and the docetaxel micelle solution (prepared by the invention) are subjected to a tumor suppression test. The testing object is Balb/c mice with transplanted L7912 tumor strain. The administration route is intravenous administration, one time every three days, and administered continuously for 30 days. The drug concentration of Taxotere is same as docetaxel of 10 mg/kg of the present invention. The tumor volume in mice was measured twice every week, and the results as shown in Table 12. The results show that the tumor volume in mice with the saline drip is rapidly grown. The growing speed of the tumor volume in mice with the Taxotere drip can be controlled, but the tumor volume is still growing. The tumor volume in mice with the docetaxel of the invention is controlled and reduced quickly, as shown in Table 12. Accordingly, the docetaxel of the invention for the T cell leukemia tumor in mice has good inhibitory effect. 
         [0000]    
       
         
               
             
               
               
               
               
             
               
               
               
               
               
               
             
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
             
           
               
                 TABLE 12 
               
             
             
               
                   
               
               
                 the measurements result for the tumor volume in mice 
               
             
          
           
               
                   
                 Physiological 
                   
                 Docetaxel 
               
               
                   
                 saline 
                 Commerical docetaxel 
                 of the invention 
               
             
          
           
               
                   
                 Tumor 
                   
                 for injection 
                 Tumor 
                   
               
             
          
           
               
                 Time 
                 volume 
                   
                 Tumor volume 
                   
                 volume 
                   
               
               
                 (day) 
                 (Mm 3 ) 
                 variance 
                 (Mm 3 ) 
                 variance 
                 (Mm 3 ) 
                 variance 
               
               
                   
               
             
          
           
               
                 1 
                 165.7 
                 38.0 
                 156.0 
                 13.0 
                 157.1 
                 28.0 
               
               
                 5 
                 246.0 
                 79.0 
                 177.0 
                 7.5 
                 127.0 
                 31.5 
               
               
                 8 
                 307.0 
                 88.0 
                 154.0 
                 8.3 
                 56.8 
                 16.0 
               
               
                 12 
                 401.0 
                 149.0 
                 157.0 
                 4.3 
                 18.0 
                 6.0 
               
               
                 15 
                 466.0 
                 124.0 
                 175.0 
                 3.8 
                 8.8 
                 2.0 
               
               
                 19 
                 552.0 
                 90.0 
                 178.0 
                 7.3 
                 4.1 
                 1.8 
               
               
                 22 
                 642.0 
                 79.6 
                 187.9 
                 13.9 
                 1.6 
                 0.9 
               
               
                 26 
                 707.9 
                 44.9 
                 226.7 
                 27.5 
                 1.9 
                 1.0 
               
               
                 30 
                 861.3 
                 110.7 
                 305.2 
                 52.0 
                 2.8 
                 2.0 
               
               
                   
               
             
          
         
       
     
       Examples 17-23 
       [0061]    Docetaxel is anhydrous docetaxel (CAS: 114977-28-5), which is produced by Xi&#39;an Tianfeng natural field Bio-Technique Co., Ltd. 
         [0062]    In Examples 17-23, polyethylene glycol methyl ether-polylactide block copolymer with amino acid end groups is polyethylene glycol methyl ether-polylactide-glutamic acid, and the chemical formula of polyethylene glycol methyl ether-polylactide-glutamic acid as follows: 
         [0000]    
       
                 
         
             
             
         
       
     
         [0000]    wherein the subscript a in formula (IV) is ranging from 10-200, the subscript b in formula (IV) is ranging from 3-30. For polyethylene glycol methyl ether-polylactide-glutamic acid, the average molecular weight of polylactide block is ranging from 504-4985 and the average molecular weight of polyethylene glycol methyl ether block is ranging from 1003-3015. Polyethylene glycol methyl ether-polylactide-glutamic acid is prepared according to China patent application No. 2013000453. The average molecular weight of different blocks is determined by mass spectrum analysis with molecular weight of the materials. 
         [0063]    In Examples 17-23, the pharmaceutical component of pharmaceutical component containing docetaxel is micelles, which is prepared by film hydration method. The steps of the method include:
   (1) Docetaxel and polyethylene glycol methyl ether-polylactide-glutamic acid are weighted respectively according to different feed ratios (see Table 13).   (2) The above raw materials (which include docetaxel and polyethylene glycol methyl ether-polylactide-glutamic acid) are disposed into a container, and an organic solvent is added into container until the raw materials dissolved completely, in which the organic solvent includes ethanol, acetonitrile and the like. The container is rotated and evaporated under 30-50° C. for 2 hours until the organic solvent was evaporated to dryness. The remaining organic solvent is dried in vacuo at 10-60° C. and is performed more than 12 hours, so as to the polymeric mixed film of docetaxel-containing can be obtained.   (3) The polymer mixed film is disposed in a water bath with a temperature at 40-60° C. until the polymer mixed film becomes the transparent. Then, preheated ultrapure water, physiological saline, or phosphate buffer with same temperature as the polymer mixed film is added into the polymer mixed film (disposed in water bath) and is shaken and hydrated well. Thus, the transparent drug loading micellar solution is obtained.   (4) The drug loading micelle solution is filtered with 0.45 μm filter membrane to obtain micelles.   (5) The drug loading micelle is subjected to lyophilization process without adding any excipient to obtain the solid freeze-dried powder.   
 
         [0069]    The preparation method of the freeze-dried powder as follows: 
         [0000]    Step a: a shelf is performed pre-cooling process to 0° C. and the docetaxel micelles is disposed on the shelf to cool to below −45° C., so as to the solution temperature with docetaxel micelles is dropped to −45° C., and the solution is maintained at a temperature −45° C. for 2-4 hours.
 
Step b; the vacuum pump is turned on and vacuumed for 1-1.5 hours after the step a.
 
Step c: the temperature of the spacer plate is set from −45° C. to −25° C. and the temperature of the spacer plate is maintained more than 12 hours. The ultimate vacuum is set at 0.013 Bar.
 
Step d: the temperature of the spacer plate is set from −25° C. to 0° C. and the temperature of the spacer plate is maintained more than 8 hours. The ultimate vacuum is set at 0.013 Bar.
 
Step e: the temperature of the spacer plate is set from 0° C. to 5° C. and the temperature of the spacer plate is maintained more than 3 hours. The ultimate vacuum is set at 0.013 Bar.
 
Step f: the temperature of the spacer plate is set from 5° C. to 20° C. After the temperature of the reached at 20° C., the temperature of the product is maintained more than 4 hours. Then, the lyophilization process is finished and the freeze-dried powder with loose block can be obtained.
 
         [0070]    In addition, according to above steps, an additional step (6) to add an excipient to prepare a freeze-dried powder as follows: 
         [0071]    The excipient is added into the docetaxel micelle and the total weight of the excipient is not exceeding 5% of above step (1), and the excipient and the docetaxel micelle are fully dissolved. Next, according to above step (5), the lyophilization process is performed to the mixture of the excipient and the docetaxel micelle to prepare the docetaxel freeze-dried powder. The excipient is selected from the group consisting of one or more of lactose, mannitol, dextran, glycine, and glucose. 
         [0072]    In above preparation, the organic solvent is ethanol or acetonitrile in above step (2). 
         [0073]    The role of excipients in the lyophilization process is to promote the solution which is difficult to be solidified, so as to the solid powder can be obtained. The various excipients can also improve the performance indicators such as appearance, shape, solubility, and stability of the docetaxel freeze-dried powder according to varying degree. 
         [0074]    Examples 17-23 select the different feed ratios and the polyethylene glycol methyl ether-polylactide-glutamic acid with different molecular weight, which is prepared in accordance with the above procedure to obtain the docetaxel freeze-dried powder. The amount of the drug loading is determined by HPLC and the average particle size of the solution which is redissolved is determined by dynamic light scattering and as shown in Table 13. 
         [0000]    
       
         
               
             
               
               
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 13 
               
             
             
               
                   
               
               
                 the measurement result for amount of drug loading and particle size in 
               
               
                 different examples. 
               
             
          
           
               
                   
                 Polyethylene 
                   
                   
                   
                 Average 
               
               
                   
                 glycol methyl 
                   
                   
                 Acutal drug 
                 particele 
               
               
                   
                 ether 
                 Polylactide 
                 Feed ratio 
                 loading 
                 size 
               
               
                 Example 
                 M.W. 
                 M.W. 
                 Docetaxel:carrier 
                 (Docetaxel:carrier) 
                 (nm) 
               
               
                   
               
             
          
           
               
                 Example 17 
                 1989 
                 842 
                  4:100 
                 3.43:100 
                 21.5 
               
               
                 Example 18 
                 1998 
                 1419 
                  8:100 
                 6.67:100 
                 28.9 
               
               
                 Example 19 
                 2009 
                 1685 
                 16:100 
                 12.55:100  
                 27.6 
               
               
                 Example 20 
                 1996 
                 1905 
                 30:100 
                 26.78:100  
                 26.2 
               
               
                 Example 21 
                 2014 
                 3256 
                 20:100 
                 17.85:100  
                 25.3 
               
               
                 Example 22 
                 1003 
                 504 
                 10:100 
                 8.90:100 
                 27.2 
               
               
                 Example 23 
                 3015 
                 4985 
                  6:100 
                 4.20:100 
                 26.6 
               
               
                   
               
               
                 Carrier = polyethylene glycol methyl ether-polylactide-glutamic acid 
               
             
          
         
       
     
         [0075]    In accordance with the ratio of 3 mg/ml concentration of docetaxel, the docetaxel freeze-dried powder and water are weighted respectively which is prepared by Examples 17-23. The above freeze-dried powder is fed into the water for injection, the physiological saline or glucose for injection. Next, the docetaxel freeze-dried powder is fully dissolved after shaking with 60 seconds, so as to the docetaxel freeze-dried powder indicates good solubility. The average particle size distribution of the solution (or called redissolved solution) with docetaxel freeze-dried powder is determined, and the particle size of the redissolved solution is ranging from 10-100 nm, an average particle size is ranging from 20-29.2 nm, as shown in Table 14. The redissolved solution is respectively observered at 15° C., 25° C., 30° C. and under the normal indoor lighting condition every 2 hours, until the solution appears cloudy or the precipitation was found. The observation result shows that the end of the steady state of the redissolved solution. The results are shown in Table 14, which illustrate the docetaxel freeze-dried powder of the present invention has good stability. 
         [0000]    
       
         
               
             
               
               
               
               
             
               
               
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 14 
               
             
             
               
                   
               
               
                 the test results for the average particle size and the stability 
               
               
                 for the redissolved soltion in different Examples 
               
             
          
           
               
                   
                   
                 Average 
                   
               
               
                   
                 Redissolved 
                 particle 
                 Settling time (Hour) 
               
             
          
           
               
                 Example 
                 solvent 
                 size (nm) 
                 15° C. 
                 25° C. 
                 30° C. 
               
               
                   
               
             
          
           
               
                 Example 17 
                 Water for 
                 21.5 
                 26 
                 14 
                 6 
               
               
                   
                 injection 
               
               
                 Example 18 
                 0.9% NaCl 
                 28.9 
                 32 
                 24 
                 14 
               
               
                 Example 19 
                 5.0% Glucose 
                 27.6 
                 28 
                 14 
                 8 
               
               
                 Example 20 
                 0.9% NaCl 
                 26.2 
                 28 
                 12 
                 6 
               
               
                 Example 21 
                 Water for 
                 25.3 
                 24 
                 12 
                 6 
               
               
                   
                 injection 
               
               
                 Example 22 
                 Water for 
                 27.2 
                 18 
                 12 
                 10 
               
               
                   
                 injection 
               
               
                 Example 23 
                 Water for 
                 26.6 
                 16 
                 16 
                 8 
               
               
                   
                 injection 
               
               
                   
               
             
          
         
       
     
         [0076]    Stability Test: 
         [0077]    The docetaxel micelles which is prepared from Examples 17-23 is diluted with water to obtain docetaxel concentration of about 3 mg/ml. The solution (docetaxel micelles diluted with water) is respectively observered at 15° C., 25° C., 30° C. and under the normal indoor lighting condition every 2 hours, until the solution appears cloudy or the precipitation was found. The observation result shows that the end of the steady state of the solution (docetaxel micelles diluted with water). The results are shown in Table 15. 
         [0000]    
       
         
               
             
               
               
             
               
               
               
               
               
             
               
               
               
               
               
             
           
               
                 TABLE 5 
               
             
             
               
                   
               
               
                 the stability test results in different Examples 
               
             
          
           
               
                   
                 Settling time (Hour) 
               
             
          
           
               
                   
                 Example 
                 15° C. 
                 25° C. 
                 30° C. 
               
               
                   
                   
               
             
          
           
               
                   
                 Example 17 
                 26 
                 12 
                 6 
               
               
                   
                 Example 18 
                 32 
                 24 
                 12 
               
               
                   
                 Example 19 
                 28 
                 14 
                 8 
               
               
                   
                 Example 20 
                 28 
                 14 
                 6 
               
               
                   
                 Example 21 
                 24 
                 12 
                 8 
               
               
                   
                 Example 22 
                 18 
                 14 
                 10 
               
               
                   
                 Example 23 
                 16 
                 16 
                 8 
               
               
                   
                   
               
             
          
         
       
     
       Example 24 
       [0078]    The physiological saline, the commercial docetaxel for injection (Taxotere) and the docetaxel micelle solution (prepared by the invention) are subjected to a tumor suppression test. The testing object is Balb/c mice with transplanted L7912 tumor strain. The administration route is intravenous administration, one time every three days, and administered continuously for 30 days. The drug concentration of Taxotere is same as docetaxel of 10 mg/kg of the present invention. The tumor volume in mice was measured twice every week, and the results as shown in Table 16. The results show that the tumor volume in mice with the saline drip is rapidly grown. The growing speed of the tumor volume in mice with the Taxotere drip can be controlled, but the tumor volume is still growing. The tumor volume in mice with the docetaxel of the invention is controlled and reduced quickly, as shown in Table 16. Accordingly, the docetaxel of the invention for the T cell leukemia tumor in mice has good inhibitory effect. 
         [0000]    
       
         
               
             
               
               
               
               
             
               
               
               
               
               
               
             
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
             
           
               
                 TABLE 16 
               
             
             
               
                   
               
               
                 the measurements result for the tumor volume in mice 
               
             
          
           
               
                   
                 Physiological 
                   
                 Docetaxel 
               
               
                   
                 saline 
                 Commerical docetaxel 
                 of the invention 
               
             
          
           
               
                   
                 Tumor 
                   
                 for injection 
                 Tumor 
                   
               
             
          
           
               
                 Time 
                 volume 
                   
                 Tumor volume 
                   
                 volume 
                   
               
               
                 (day) 
                 (Mm 3 ) 
                 variance 
                 (Mm 3 ) 
                 variance 
                 (Mm 3 ) 
                 variance 
               
               
                   
               
             
          
           
               
                 1 
                 165.7 
                 38.0 
                 156.0 
                 13.0 
                 158.0 
                 22.0 
               
               
                 5 
                 246.0 
                 79.0 
                 177.0 
                 7.5 
                 128.1 
                 31.1 
               
               
                 8 
                 307.0 
                 88.0 
                 154.0 
                 8.3 
                 54.7 
                 18.8 
               
               
                 12 
                 401.0 
                 149.0 
                 157.0 
                 4.3 
                 18.0 
                 5.1 
               
               
                 15 
                 466.0 
                 124.0 
                 175.0 
                 3.8 
                 7.8 
                 2.2 
               
               
                 19 
                 552.0 
                 90.0 
                 178.0 
                 7.3 
                 3.0 
                 1.2 
               
               
                 22 
                 642.0 
                 79.6 
                 187.9 
                 13.9 
                 1.6 
                 0.9 
               
               
                 26 
                 707.9 
                 44.9 
                 226.7 
                 27.5 
                 1.7 
                 1.4 
               
               
                 30 
                 861.3 
                 110.7 
                 305.2 
                 52.0 
                 2.6 
                 2.0 
               
               
                   
               
             
          
         
       
     
       Examples 25-31 
       [0079]    Docetaxel is anhydrous docetaxel (CAS: 114977-28-5), which is produced by Xi&#39;an Tianfeng natural field Bio-Technique Co., Ltd. 
         [0080]    In Examples 25-31, polyethylene glycol methyl ether-polylactide block copolymer with amino acid end groups is polyethylene glycol methyl ether-polylactide-phenylalanine, and the chemical formula of polyethylene glycol methyl ether-polylactide-phenylalanine as follows 
         [0000]    
       
                 
         
             
             
         
       
     
         [0000]    wherein the subscript a in formula (V) is ranging from 10-200, the subscript b in formula (V) is ranging from 3-30. For polyethylene glycol methyl ether-polylactide-phenylalanine, the average molecular weight of polylactide block is ranging from 502-4962 and the average molecular weight of polyethylene glycol methyl ether block is ranging from 998-2998. Polyethylene glycol methyl ether-polylactide-phenylalanine is prepared according to China patent application No. 2013000453. The average molecular weight of different blocks is determined by mass spectrum analysis with molecular weight of the materials. 
         [0081]    In Examples 25-31, the pharmaceutical component of pharmaceutical component containing docetaxel is micelles, which is prepared by a film hydration method. The steps of the method include:
   (1) Docetaxel and polyethylene glycol methyl ether-polylactide-phenylalanine are weighted respectively according to different feed ratios (see Table 17).   (2) The above raw materials (which include docetaxel and polyethylene glycol methyl ether-polylactide-phenylalanine) are disposed into a container, and an organic solvent is added into container until the raw materials dissolved completely, in which the organic solvent includes ethanol, acetonitrile and the like. The container is rotated and evaporated under 30-50° C. for 2 hours until the organic solvent was evaporated to dryness. The remaining organic solvent is dried in vacuo at 10-60° C. and is performed more than 12 hours, so as to the polymeric mixed film of docetaxel-containing can be obtained.   (3) The polymer mixed film is disposed in a water bath with a temperature at 40-60° C. until the polymer mixed film becomes the transparent. Then, preheated ultrapure water, physiological saline, or phosphate buffer with same temperature as the polymer mixed film is added into the polymer mixed film (disposed in water bath) and is shaken and hydrated well. Thus, the transparent drug loading micellar solution is obtained.   (4) The drug loading micelle solution is filtered with 0.45 μm filter membrane to obtain micelles.   (5) The drug loading micelle is subjected to lyophilization process without adding any excipient to obtain the solid freeze-dried powder.   
 
         [0087]    The preparation method of the freeze-dried powder as follows: 
         [0000]    Step a: a shelf is performed pre-cooling process to 0° C. and the docetaxel micelles is disposed on the shelf to cool to below −45° C., so as to the solution temperature with docetaxel micelles is dropped to −45° C., and the solution is maintained at a temperature −45° C. for 2-4 hours.
 
Step b: the vacuum pump is turned on and vacuumed for 1-1.5 hours after the step a.
 
Step c: the temperature of the spacer plate is set from −45° C. to −25° C. and the temperature of the spacer plate is maintained more than 12 hours. The ultimate vacuum is set at 0.013 Bar.
 
Step d: the temperature of the spacer plate is set from −25° C. to 0° C. and the temperature of the spacer plate is maintained more than 8 hours. The ultimate vacuum is set at 0.013 Bar.
 
Step e: the temperature of the spacer plate is set from 0° C. to 5° C. and the temperature of the spacer plate is maintained more than 3 hours. The ultimate vacuum is set at 0.013 Bar.
 
Step f: the temperature of the spacer plate is set from 5° C. to 20° C. After the temperature of the reached at 20° C., the temperature of the product is maintained more than 4 hours. Then, the lyophilization process is finished and the freeze-dried powder with loose block can be obtained.
 
         [0088]    In addition, according to above steps, an additional step (6) to add an excipient to prepare a freeze-dried powder as follows: 
         [0089]    The excipient is added into the docetaxel micelle and the total weight of the excipient is not exceeding 5% of above step (1), and the excipient and the docetaxel micelle are fully dissolved. Next, according to above step (5), the lyophilization process is performed to the mixture of the excipient and the docetaxel micelle to prepare the docetaxel freeze-dried powder. The excipient is selected from the group consisting of one or more of lactose, mannitol, dextran, glycine, and glucose. 
         [0090]    In above preparation, the organic solvent is ethanol or acetonitrile in above step (2). 
         [0091]    The role of excipients in the lyophilization process is to promote the solution which is difficult to be solidified, so as to the solid powder can be obtained. The various excipients can also improve the performance indicators such as appearance, shape, solubility, and stability of the docetaxel freeze-dried powder according to varying degree. 
         [0092]    Examples 25-31 select the different feed ratios and the polyethylene glycol methyl ether-polylactide-phenylalanine with different molecular weight, which is prepared in accordance with the above procedure to obtain the docetaxel freeze-dried powder. The amount of the drug loading is determined by HPLC and the average particle size of the solution which is redissolved is determined by dynamic light scattering, as shown in Table 17. 
         [0000]    
       
         
               
             
               
               
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 17 
               
             
             
               
                   
               
               
                 the measured amount of the drug loading and particle size in different 
               
               
                 examples 
               
             
          
           
               
                   
                 Polyethylene 
                   
                   
                   
                 Average 
               
               
                   
                 glycol methyl 
                   
                   
                   
                 particele 
               
               
                   
                 ether 
                 Polylactide 
                 Feed ratio 
                 Acutal drug loading 
                 size 
               
               
                 Example 
                 M.W. 
                 M.W. 
                 Docetaxel:carrier 
                 (Docetaxel:carrier) 
                 (nm) 
               
               
                   
               
             
          
           
               
                 Example 25 
                 1996 
                 847 
                  4:100 
                 3.57:100 
                 18.5 
               
               
                 Example 26 
                 2008 
                 1420 
                  8:100 
                 5.42:100 
                 22.6 
               
               
                 Example 27 
                 1989 
                 1678 
                 16:100 
                 15.02:100  
                 23.5 
               
               
                 Example 28 
                 1993 
                 1909 
                 30:100 
                 25.76:100  
                 24.2 
               
               
                 Example 29 
                 2013 
                 3230 
                 20:100 
                 16.98:100  
                 24.7 
               
               
                 Example 30 
                 998 
                 502 
                 10:100 
                 8.46:100 
                 24.1 
               
               
                 Example 31 
                 2988 
                 4962 
                  6:100 
                 4.42:100 
                 24.2 
               
               
                   
               
               
                 Carrier: polyethylene glycol methyl ether-polylactide-phenylalanine 
               
             
          
         
       
     
         [0093]    In accordance with the ratio of 3 mg/ml concentration of docetaxel, the docetaxel freeze-dried powder and water are weighted respectively which is prepared by Examples 25-31. The above freeze-dried powder is fed into the water for injection, the physiological saline or glucose for injection. Next, the docetaxel freeze-dried powder is fully dissolved after shaking with 60 seconds, so as to the docetaxel freeze-dried powder indicates good solubility. The average particle size distribution of the solution (or called redissolved solution) with docetaxel freeze-dried powder is determined, and the particle size of the redissolved solution is ranging from 10-100 nm, an average particle size is ranging from 20-29.2 nm, as shown in Table 18. The redissolved solution is respectively observered at 15° C., 25° C., 30° C. and under the normal indoor lighting condition every 2 hours, until the solution appears cloudy or the precipitation was found. The observation result shows that the end of the steady state of the redissolved solution. The results are shown in Table 18, which illustrate the docetaxel freeze-dried powder of the present invention has good stability. 
         [0000]    
       
         
               
             
               
               
               
               
             
               
               
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 18 
               
             
             
               
                   
               
               
                 the test results for the average particle size and the stability 
               
               
                 for the redissolved soltion in different Examples 
               
             
          
           
               
                   
                   
                 Average 
                   
               
               
                   
                 Redissolved 
                 particle size 
                 Settling time (Hour) 
               
             
          
           
               
                 Example 
                 solvent 
                 (nm) 
                 15° C. 
                 25° C. 
                 30° C. 
               
               
                   
               
             
          
           
               
                 Example 25 
                 Water for 
                 18.5 
                 26 
                 14 
                 6 
               
               
                   
                 injection 
               
               
                 Example 26 
                 0.9% NaCl 
                 22.6 
                 32 
                 24 
                 14 
               
               
                 Example 27 
                 5.0% 
                 23.5 
                 28 
                 14 
                 8 
               
               
                   
                 Glucose 
               
               
                 Example 28 
                 0.9% NaCl 
                 24.2 
                 28 
                 12 
                 6 
               
               
                 Example 29 
                 Water for 
                 24.7 
                 24 
                 12 
                 6 
               
               
                   
                 injection 
               
               
                 Example 30 
                 Water for 
                 24.1 
                 18 
                 12 
                 10 
               
               
                   
                 injection 
               
               
                 Example 31 
                 Water for 
                 24.2 
                 16 
                 16 
                 8 
               
               
                   
                 injection 
               
               
                   
               
             
          
         
       
     
         [0094]    Stability Test: 
         [0095]    The docetaxel micelles which is prepared from Examples 25-31 is diluted with water to obtain docetaxel concentration of about 3 mg/ml. The solution (docetaxel micelles diluted with water) is respectively observered at 15° C., 25° C., 30° C. and under the normal indoor lighting condition every 2 hours, until the solution appears cloudy or the precipitation was found. The observation result shows that the end of the steady state of the solution (docetaxel micelles diluted with water). The results are shown in Table 19. 
         [0000]    
       
         
               
             
               
               
             
               
               
               
               
               
             
               
               
               
               
               
             
           
               
                 TABLE 19 
               
             
             
               
                   
               
               
                 the stability test results in different Examples 
               
             
          
           
               
                   
                 Settling time (Hour) 
               
             
          
           
               
                   
                 Example 
                 15° C. 
                 25° C. 
                 30° C. 
               
               
                   
                   
               
             
          
           
               
                   
                 Example 25 
                 26 
                 12 
                 6 
               
               
                   
                 Example 26 
                 32 
                 18 
                 12 
               
               
                   
                 Example 27 
                 28 
                 14 
                 10 
               
               
                   
                 Example 28 
                 28 
                 14 
                 8 
               
               
                   
                 Example 29 
                 24 
                 12 
                 8 
               
               
                   
                 Example 30 
                 18 
                 14 
                 10 
               
               
                   
                 Example 31 
                 16 
                 16 
                 8 
               
               
                   
                   
               
             
          
         
       
     
       Example 32 
       [0096]    The physiological saline, the commercial docetaxel for injection (Taxotere) and the docetaxel micelle solution (prepared by the invention) are subjected to a tumor suppression test. The testing object is Balb/c mice with transplanted L7912 tumor strain. The administration route is intravenous administration, one time every three days, and administered continuously for 30 days. The drug concentration of Taxotere is same as docetaxel of 10 mg/kg of the present invention. The tumor volume in mice was measured twice every week, and the results as shown in Table 20. The results show that the tumor volume in mice with the saline drip is rapidly grown. The growing speed of the tumor volume in mice with the Taxotere drip can be controlled, but the tumor volume is still growing. The tumor volume in mice with the docetaxel of the invention is controlled and reduced quickly, as shown in Table 20. Accordingly, the docetaxel of the invention for the T cell leukemia tumor in mice has good inhibitory effect. 
         [0000]    
       
         
               
             
               
               
               
               
             
               
               
               
               
               
               
             
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
             
           
               
                 TABLE 20 
               
             
             
               
                   
               
               
                 the measurements result for the tumor volume in mice 
               
             
          
           
               
                   
                 Physiological 
                   
                 Docetaxel 
               
               
                   
                 saline 
                 Commerical docetaxel 
                 of the invention 
               
             
          
           
               
                   
                 Tumor 
                   
                 for injection 
                 Tumor 
                   
               
             
          
           
               
                 Time 
                 volume 
                   
                 Tumor volume 
                   
                 volume 
                   
               
               
                 (day) 
                 (Mm 3 ) 
                 variance 
                 (Mm 3 ) 
                 variance 
                 (Mm 3 ) 
                 variance 
               
               
                   
               
             
          
           
               
                 1 
                 165.7 
                 38.0 
                 156.0 
                 13.0 
                 156.0 
                 32.0 
               
               
                 5 
                 246.0 
                 79.0 
                 177.0 
                 7.5 
                 127.0 
                 31.0 
               
               
                 8 
                 307.0 
                 88.0 
                 154.0 
                 8.3 
                 57.0 
                 17.0 
               
               
                 12 
                 401.0 
                 149.0 
                 157.0 
                 4.3 
                 17.0 
                 5.0 
               
               
                 15 
                 466.0 
                 124.0 
                 175.0 
                 3.8 
                 8.3 
                 1.7 
               
               
                 19 
                 552.0 
                 90.0 
                 178.0 
                 7.3 
                 2.7 
                 1.4 
               
               
                 22 
                 642.0 
                 79.6 
                 187.9 
                 13.9 
                 1.4 
                 0.9 
               
               
                 26 
                 707.9 
                 44.9 
                 226.7 
                 27.5 
                 1.9 
                 1.4 
               
               
                 30 
                 861.3 
                 110.7 
                 305.2 
                 52.0 
                 2.9 
                 2.2 
               
               
                   
               
             
          
         
       
     
         [0097]    While the invention has been described in terms of what is presently considered to be the most practical and preferred embodiments, it is to be understood that the invention needs not be limited to the disclosed embodiments. On the contrary, it is intended to cover various modifications and similar arrangements included within the spirit and scope of the appended claims which are to be accorded with the broadest interpretation so as to encompass all such modifications and similar structures.