Abstract:
A Chinese herbal composition for environmental sanitation is free of toxin and user-friendly water-soluble spray. The practical applications of this spray include pest annihilation, better environmental sanitation, germicide and viruses inhibitive functions. It is also effective in prevention against epidemic and prevalent disease. This composition is composed of  Akebia trifoliata, Polygonum perifoliatum, Euodia rutaecarpa, Stemona tuberose, Bryophyllum pinnatum,  and  Sophora flavescens.  These drugs have passed the western and oriental pharmacologists&#39; pharmacological experiments and proved of pesticide, germicide and viruses inhibitive effects. As based on above, the inventor also concerns the multiple effects of drug composition. By using revolutionary extraction and scientific preparation methods, the non-toxic Chinese herbal ingredients can be transformed into potent annihilative preparation.

Description:
BACKGROUND OF THE INVENTION  
       [0001]     1. Field of the Invention  
         [0002]     The present invention relates generally to medicine, and more particularly to a Chinese herbal composition used in environmental sanitation.  
         [0003]     2. Description of the Related Art  
         [0004]     To repel flies, gnat or mosquitoes, people used to burn the primitive burning grass for smoke; afterwards, they used to burn  Acorus calamus, Euodia rutaecarpa, Atratylodis rhizona,  Sandal wood, or sprayed realgar wine. There are some delicate actions like burning incenses and carrying pack of herbs for repelling the pests in ancient China. It is obvious that the ideas and solutions of environmental sanitation have been developed from ancient time. Following the progress of era, human have had the needs for simpleness and effectiveness since the twentieth century, thereby making the evolution of sprays, coils, electric coils and many other types of pest control preparations commercially available for annihilation mosquitoes, repelling flies, and eradiating cockroaches. Although they belong to the products of science, they are not fully confirmed to be effective. Something in common is that they are poisonous. During the period of SARS (severe acute respiratory syndrome), many patients and medical crews died for the closed disinfection method mistakenly used in one province of China.  
         [0005]     The environmental sanitary products in the market have single or limited usage.  
         [0006]     Products which have germicide and viruses inhibition were paid special attentions by the authorities as things became serious. They are expensive and hard to purchase in usual time. Many people even do not know how to use them.  
         [0007]     The environmental sanitary sprays mostly contain deadly poisons but the manufacturer labeled them with poisonous and cautions only. They don&#39;t care or ignore the consequences of the remaining poisons. Since the present inventor is a cancer researcher, in many of his cases, he has found many direct connections between the usage of this kind of pesticide and the issue of cancers. Contacting mucosa by nose and lungs will cause the defense cells to mutate into caner cells.  
         [0008]     Does it necessary for drugs to possess poisons to kill pests and eradiate bacteria and inhibit viruses? This idea is not in accordance with the facts. You can find so many non-toxic plants or ingredients from the books of Chinese medicine but still have the effects of pesticide, germicide and viruses inhibition. Especially in the 1950s, under the projects of policies and efforts by the experts, completion of experiments of many drugs and the analysis of effective ingredients have been done for the Chinese medicine. All of these contributions give the Chinese herbal medicine the opportunities of further development. How to use the information contributed by the modem experts and obey the theories of Chinese medicine to push non-toxic, complicated ingredients, less potent, and less pesticidally effective Chinese herbs to exceed poisonous agents in the contemporary worlds? It was only a wonderful dream before, but now this dream will come true.  
       SUMMARY OF THE INVENTION  
       [0009]     The primary objective of the present invention is to provide a Chinese herbal composition, which employs revolutionary extract methods in common applications of environmental sanitation to kill or to repel the common pests like mosquitoes, flies, cockroaches, lice, fleas and mites. It also includes the effects to eradiate many bacteria, fungus and viruses inhibition and possess the objective to prevent epidemic and confluent diseases.  
         [0010]     The secondary objective of the present invention is to employ the fact recited below to break the arbitrarily mode of “annihilation pests and bacteria requires poison”, and to develop the application values of using mild and gentle Chinese medicine. The present invention can replace poisonous chemicals and maintain human safety and health  
         [0011]     To achieve the foregoing objectives, I supply the theories and applications, drug dispensing, drug preparation method, pharmacology description, range and method of application, caution, and detail drug description. 
     
    
     DETAILED DESCRIPTION OF PREFERRED EMBODIMENTS  
       [0012]     The entities of the present invention are based on the implementation of “original theories and its revolution” from Chinese medicine. The applicable features of its contents include “pharmacology experiments of modem Chinese herbs” and “the right idea on environ of target creatures exist”. I use them to make the “requirements of annihilation” and develop the evolutional extract form and scientific methods of preparation. The completion of the present invention depends on such delicate steps and I will specify them below.  
         [0013]     Scientific researches about bacteria and viruses, no matter modem or the western type, all trends to the retrograde searching of their real form and source of parasite. The Chinese medicine is focused on the environmental factors and the effects to human bodies. The forms of them are different but the goals are the same. The books of Chinese medicine contain many methods of dealing with bacteria and viruses. The methods have been being used since the ancient time. The bacteria and viruses were named by the six evils of as wind, cols, summer heat, dampness, dryness, fire and their combination. “Worms are hatched from dampness and heat” is the common statement of Chinese medicine. The Chinese always put clothes, quivers and groceries under sunshine after the rainy season. The purpose of doing this is to evaporate dampness and annihilation so-called yin-evils by sunshine. The so-called yin-evil is the bacteria we know. However, many people do not understand such things to hardly believe them. In light of this, an innovative statement was proposed, reciting that “Every creature is composed by six elements such as energy, space, substance, dampness, airflow, and temperature”. The bacteria and viruses belong to creatures and are composed by the six elements and will be dead without any of the six elements. The cooperation of dampness and temperature can generate gases from spaces in the substance, such gases including tiny substance, which is the so-called bacteria and viruses harmful to the living creatures. Depend on the proportions of the six elements, there are thousands even more bacteria and viruses had been category and named or not. Therefore, I said from the pest and bacteria annihilation and viruses inhibition physiology of Chinese medicine, the point of this strategy is the content of six elements and changes of environ. In other words, by choosing adequate drugs and using their effective ingredients can promote transformation of worms, bacteria and viruses&#39; six elements immediately and change the status of the environment they exist. The goal of annihilation is achieved no matter toxic or non-toxic.  
         [0014]     The Chinese medicine is the masterpiece that has collected a great number of medical experiences of thousands of years. Based on values of the Chinese medicine and to bring connection with the modem science, the Chinese government has been devoted to the pharmacological experiments of Chinese medicine and the arrangement and compilation of related knowledge. The achievement of the above works is very remarkable. It offers the precious information and the convenience of applications to bio-technique researchers. In the present invention, I adopt the analysis about the ingredients and results and data of the aforementioned pharmacological experiments. In the situation of understanding the effective ingredients, they can be well preserved and utilized during the process of preparation. Since the pharmacological experiments are quite credible, I am sure that they can make up for the deficiency of research instruments and meet the requirements of the invention. For example, the decoct of  Buodia rutaecarpa  shows stronger inhibiting effects on  V. cholerae.  It also shows certain degree of inhibiting effects on  Pseudomonas aeruginosa, Staphylococcus aureaus  and some common disease-causing fungus. (W-F, Dam etc. Chinese medicine 1952, 38(4):315) The evodiamine shows anti-viruses effect on rats, which were infected by Columbia S K strand of viruses. (Chou S C. CA, 1967, 67:61622j)  
         [0015]     The management of oral medicine for curing disease and topical usage to kill microbe for the environment are quite different. There must have clear idea to realize the situation of pests, lice, bacteria and viruses hosted or spread in the environment. Then, we can kill or repel them reasonably. The pests, lice, bacteria and viruses have the same existing condition in the environment. They must have comfortable shields and they will disappear as we place these shields under strong streams or sunshine.  
         [0016]     In addition, the pests mainly on the damp ground which are dark and less ventilated corners are subject to reproduction. The bacteria mostly attach themselves to something under shadow after leaving their hosts. The viruses re-disperse into the air from the hosts, too. As the size of the creatures is concerned, the worm and lice are the biggest one, bacterium is the next biggest, and virus is the least one. However, as regards the capabilities of spreading themselves in the environment are contrary to the size. The worm and lice are the least, bacterium is the next, and virus is the greatest. Tiny bacteria, especially viruses, are always circulating between the shields with air. It must have something extremely tiny and dense material, then perform close contact with it. From this point, we can recall the density of Chinese incense and its volatile substance. However, the incense has limited bactericidal effects and it tends to hurt human bodies like the toxic agents.  
         [0017]     The eradication of pests and microbes in the environment has some criteria. Except carefully selecting, composing, dispensing, and testing drugs, we have to analyze these criteria. Because the targets are tiny, there must be some tinier drugs for contacting them and having sufficient penetrability after the contact to transform the targets&#39; inside (six elements) immediately. Drugs possess such effects are qualified for the criteria. In addition, it must change the atmosphere in the range of the shield to elongate and enhance the potency of drugs and let it not suitable for the targets to reproduce.  
         [0018]     For the reasons as stated above, the present invention is through the composition of individually potency-tested and potency-multiplied drugs and the process of complicated extraction to be micronized for abstracting many oily essence that can be emulsified to reduce the surface and interface tensions, and then transform it into water soluble emulsion. This make it become the most tiny and penetratable preparation. This new kind of annihilation method by emulsion is the mechanism to enhance the potency. The result of volatile soluble in water can elongate time of evaporation. It can perform some lasting atmosphere, good to human and animals but not for worm, lice, bacteria and viruses in the shielded range of target  
         [0019]     The Chinese composition spray agent in according with the present invention for environmental sanitation is prepared by Chinese herbs listed below, and then I concentrate the extraction and make powder by ratio with specific ratio of percolate. I pulverize them by grinding and sieving with emulsified agents added.  
                                                                                                                   (1) Concentrated Chinese herbal powder and its ratio:                  Akebia trifoliate     80-120   gm             Stemona tuberose     80-120   gm             Polygonum perfoliatum     80-120   gm             Bryophyllum pinnatum     80-120   gm             Euodia rutaecarpa     60-100   gm                  Sophora flavescens     pulverized as adjuvants.            (2) Percolate and its ratio:                distilled water   60-80%               glycerin   8-12%           alcohol   12-24%            (3) Emulsified agents used in original solution Of 3000 ml:                Gum Arabic   30-50   gm.             Perilla frutescens  oil   30-45   ml.             Pogostemon cablin  oil   120-35   ml.           Sandalwood oil   15-25   ml                      
 
         [0020]     There are three steps recited below for preparation of the present invention.  
         [0021]     (1) Take concentrated Chinese composition powder 600 gm and percolate as well. For the first time, douse the powder in the percolate of 1200 ml then pass it to a golden mill for 2-3 times, douse for 2 hours, and leach the first percolate. For the second time, douse the same powder in the percolate of 1000 ml for 2 hours, and then leach the second percolate. For the third time, douse the power in distilled water of 1000 ml for 4 hours and then leach the third percolate. Thus, there are totally three percolates for later use.  
         [0022]     (2) Take gum Arabic,  Perilla frutescens  oil,  Pogostemon cablin  oil and Sandalwood oil to make emulsion. Blend the emulsion into the percolate of the step (1) and let it through the golden mill for three times to get a percolate.  
         [0023]     (3) Blend Mirabilite, benzoic acid, and other additives into the percolate of the step (2). Stir the admixture evenly and then let it through the golden mill for 2-3 times to get the agent of the present invention.  
         [0024]     The pharmacological specification is recited below.  
         [0025]     1.  Akebia trifoliate:  The  Akebia trifoliate  shows inhibitive effects on Gram&#39;s positive and Gram&#39;s negative bacteria such as  Shigella  and  Salmonella typhi  in vitro. It contains glycosides and saponins which can produce multiple effects.  
         [0026]     2.  Polygonum perfoliatum:  The  Polygonum perifoliatum  shows the anti-virus potency against Asian A-type influenza viruses and para-influenza I-typed viruses. The decoctant shows anti-bacterial potency against Shigella, Smith S, flexneri and  S. sonnei.  The decoctant show stronger anti-bacteria effects on  Haemophilus influenzae, Salmonella typhi, Corynebacterium diphtheriae, Bacillus anthracis, E. Coli  and β-streptococcus.  
         [0027]     3.  Euodia rutaecarpa:  It contains many alkaloids and has the annihilative effects on worms and leeches. The evodiamine shows anti-virus effect on rats which are infected by Columbia SK viruses. It shows stronger inhibitive effects on  Vibrio cholerae  in vrtrohas certain degree of inhibitive effects on  Pseudomonas aeruginosa, Staphylococcus aureaus  and some common disease-causing fungus.  
         [0028]     4.  Stemona tuberose:  It contains 13 alkaloids and 6 organic acids. It can kill body lice, pubic lice, maggots, bed bugs and many other pests. It is an apparently pesticide which can make eggs of lice and pests hard to hatch and show effects on anti-microbes.  
         [0029]     5.  Bryophyllum pinnatum:  It contains many organic acids, sugar glycosides, steroids and ketones. The juice from leaves of  Bryophyllum pinnatum  in 5% (v/v) concentration shows broad spectrum of bactericidal effects on Gram&#39;s positive and Gram&#39;s negative bacteria. It includes many resistant bacteria separated from clinical.  
         [0030]     6.  Perilla frutescens:  The decoctant of  Perilla frutescens  shows inhibitive effect on ECHO11 viruses in vitro. The perillaldehyde and tadeonal show apparently cooperative anti-fungal effects. The oil from leaves of  Perilla frutescens  shows apparently inhibitive effects on nature pollution sources of  Aspergillus niger,  Penicillium and yeasts.  
         [0031]     7.  Pogostemon cablin:  It contains pathchoulo alcohol, pathchoulo diol and many alkene volatile oil. It shows apparently inhibitive effects on fungi such as  C. albicans, Cryptococcus neoformans  and  Rhizopus stolonifer.  It shows certain degree of inhibitive effect on type A hemolytic  Streptococcus.    
         [0032]     8.  Santalum album:  The main ingredients are sesquiterpene compounds such as α-santol and β-santol up to 90% or above. It has stronger anti-bacteria effects and also has inhibitive effect on  Shigella  species and  M. avium.    
         [0033]     The range and method of application for the present invention is recited below.  
         [0034]     1. The present invention is a water soluble spray agent, having certain degree of pest annihilative, germicidal, and virus-inhibitive effects. 
        A. In pesticide: It is pesticidal to mosquito, fly, cock roach, flea, wood lice, mite and head lice, body lice, pubic lice, dog lice and eggs which located on human body surface.     B. In germicide: It is germicidal to influenza, cephalitis, hepatitis, diphtheria, dysentery, cholera and anthrax bacteria vice versa. Especially Gram&#39;s positive and Gram&#39;s negative bacteria with nature causing  Aspergillus niger  and Penicillium.     C. In viruses: It is inhibitive to Asian A-type influenza viruses and para-influenza type I viruses, Columbia SK viruses, Echo viruses and Enterovirus.        
 
         [0038]     2. It is available to manually spray the present invention in kitchen, living room, and other rooms, especially at the moist and bad ventilated corners or the places those pests gathered. It is also available to spray the present invention directly to where body lice, pubic lice, head lice or dog lice is hosted.  
         [0039]     3. The present invention can be sprayed by power sprayer on the inner corners of buildings, walls, poles and utilities or dark damp corners at hospital, clinic and public areas or in the periods of epidemic bacterial or viral infection.  
         [0040]     There are some cautions for using the present invention.  
         [0041]     1. The spray prepared by the present invention is harmless to animals and human. It does not cause any stimulation as contact with skin, eyes, nose, mouth and tongue. It will all right if a little amount of the spray is eaten. Human and animals can breathe normally and stay in closed space during the period of ejecting the spray.  
         [0042]     2. The present invention is a topically Chinese medicine pesticide, germicide and virus-inhibitive agent. It is prohibited to take it orally. It may cause gastrointestinal symptoms when taken orally. People who mistakes it can mix rare green bean powder, rare glycyrrhizin powder and talcum powder 6 gm of 3-6 gm respectively with cold water and take it orally, and thus they may feel laxative and then better.  
         [0043]     3. The present invention should be stored in cool and dry places to prevent deterioration caused by high temperature.  
         [0044]     The detailed pharmacological specification of the Chinese herbs indicated above is recited below.  
                                                           NO: 1                                HERBAL NAME   Mutong   OFFICIAL NAME     Akebia trifoliate                     (Thunb.)                    REFERENCE BASIS   China Pharmacopoeia Volume III pp 329 (China Sheng-Ghai           scientific technology publication)       GROWING DISTRICT     Akebia trifoliate  grows mainly in Si-chuan. Collect some old       &amp; COLLECT SEASON   vines in autumn and winter. Dry or bakE then slice them into       &amp; PARTS   thin pieces.       PREPARATION &amp;   Take 12 kg of  Akebia trifoliate  and soak in water for 3 hours.       EXTRACTION   Collect percolate for 2 times then evaporate the residue       PROCESS &amp; METHOD   water to get 5 liters of fluid extract. Adding 2 kg of  Sophora               flavescens  powder and stir well. Drying it in 60° C. then           pulverized by passing #120 sieve to make powder. Collect           and store them for later use.       EFFECTIVE   The vines of  Akebia trifoliate  contain betulin, oleanic acid,       INGREDIENT   hederagenin, akebin. It also contains stigmasterol, β-           sitisterol, daucosterol, inositol, sugar and sodium salts.       ORIGINAL   The extract of  Akebia trifoliate  (1:20) shows inhibitive       RECORDED   effects on Gram&#39;s positive and Gram&#39;s negative bacteria       PHARMACOLOGICAL   such as  Shigella  and  Salmonella typhi  in vitro. (Wang-yu et       EFFECTS   al. Plant report, 1953, 2(2): 312)                  
 
         [0045]    
       
         
               
             
               
               
               
               
             
               
               
             
           
               
                   
               
               
                   
               
               
                 NO: 2 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 HERBAL NAME 
                 Gangvangai 
                 OFFICIAL NAME 
                 
                   Polygonum 
                 
               
               
                   
                   
                   
                   perifoliatum  L. 
               
               
                   
               
             
          
           
               
                 GROWING DISTRICT 
                 It grows in southeast provinces of China, Fu-jann, and 
               
               
                 &amp; COLLECT SEASON 
                 Taiwan. Collect them in full blossom in summer and 
               
               
                 &amp; PARTS 
                 autumn. Cut the upper parts of plants and drying. Bundle 
               
               
                   
                 them as half drying and continue drying under shades. 
               
               
                 PREPARATION &amp; 
                 Take 12 kg of  Polygonum perifoliatum  and soak in water for 
               
               
                 EXTRACTION 
                 3 hours. Collect percolate for 2 times then evaporate the 
               
               
                 PROCESS &amp; METHOD 
                 residue water to get 5 liters of fluid extract. Adding 2 kg of 
               
               
                   
                   Sophora flavescens  powder and stir well. Drying it in 45° C. 
               
               
                   
                 then pulverized by passing #120 sieve to make powder. 
               
               
                   
                 Collect and store them for later use. 
               
               
                 EFFECTIVE 
                 The all plant contains kaemferol, methylcaffeate ester, 
               
               
                 INGREDIENT 
                 quercitin, caffeic acid, protocate chuic acid, methyl caffinate 
               
               
                   
                 ester, p-coumaric acid, feruic acid, vanillic acid, arbutinic 
               
               
                   
                 acid and betulinic acid. It also contains sterol fatty acid 
               
               
                   
                 esters, plant steroid β -d-glucoside, 3,3,4,4,-tetramethyl 
               
               
                   
                 ellagic acid, 3,3-dinethyl ellagic acid, racemic dimethyl 
               
               
                   
                 tartrate ester and long chain fatty acids. There are indicant 
               
               
                   
                 and tannic acids. 
               
               
                 ORIGINAL 
                 The viruses test out of chicken embryo shows the decoctant 
               
               
                 RECORDED 
                 of  Polygonum perifoliatum  show the anti-viruses potency 
               
               
                 PHARMACOLOGICAL 
                 against Asian type A influenza viruses and para-influenza I 
               
               
                 EFFECTS 
                 viruses are 1:160 and 1:640, respectively. (J-Y, Ma etc. 
               
               
                   
                 Shan-si new medicine, 1979, 8(12): 42) 
               
               
                   
                 The anti-bacterial potency of the decoctant to  Shigella , 
               
               
                   
                 Smith S,  flexneri  and  S. sonnei  are 1:512, 1:128, 1:64 and 
               
               
                   
                 1:15, respectively. (C-1, Lee, Fu-chien Chinese medicine, 
               
               
                   
                 1960,5(7): 310) The decoctant show stronger anti-bacteria 
               
               
                   
                 effects on  Staphylococcus aureaus , β  -streptococcus , 
               
               
                   
                   Bacillus anthracis ,  Corynebacterium diphtheriae ,  Bacillus   
               
               
                   
                   subtilis ,  E. Coli ,  Salmonella typhi ,  Pseudomonas aeruginosa   
               
               
                   
                 and  Haemophilus influenzae . (J-Y, Ma etc. Shan-si new 
               
               
                   
                 medicine, 1979, 8(12): 42. National Chinese medicine 
               
               
                   
                 pharmacopeia Vol I, edition 1, 1976, 416) 
               
               
                   
               
             
          
         
       
     
         [0046]    
       
         
               
             
               
               
               
               
             
               
               
             
           
               
                   
               
               
                   
               
               
                 NO: 3 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 HERBAL NAME 
                 Wuzhyu 
                 OFFICIAL NAME 
                 
                   Buodia rutaecarpa 
                 
               
               
                   
                   
                   
                 (juss.) 
               
               
                   
               
             
          
           
               
                 REFERENCE BASIS 
                 China Pharmacopoeia Volume IV pp 927 (China Sheng-Ghai 
               
               
                   
                 scientific technology publication) 
               
               
                 GROWING DISTRICT 
                 It grows in Qui-zhou and Kwang-si mainly. Chang-de of 
               
               
                 &amp; COLLECT SEASON 
                 Hu-nan has the best quality. Collect them as the fruits show 
               
               
                 &amp; PARTS 
                 tea-green color and the carpel do not separates. You must 
               
               
                   
                 take all spikes of fruits before the drops of dew dry. Dry 
               
               
                   
                 them and remove the handles. 
               
               
                 PREPARATION &amp; 
                 Take 12 kg of  Buodia rutaecarpa  and soak in water for 3 
               
               
                 EXTRACTION 
                 hours. Collect percolate for 2 times then evaporate the 
               
               
                 PROCESS &amp; METHOD 
                 residue water to get 5 liters of fluid extract. Adding 2 kg 
               
               
                   
                   Sophora flavescens  of powder and stir well. Drying it in 45° 
               
               
                   
                 C. then pulverized by passing #120 sieve to make powder. 
               
               
                   
                 Collect and store them for later use. 
               
               
                 EFFECTIVE 
                 The seeds contain cis-5,8-decadieneoic acid. The volatile oil 
               
               
                 INGREDIENT 
                 has evodene, evodol and limonin. The fruits contain many 
               
               
                   
                 alkaloids such as evodiamine, rutaecarpine, alkenes, ketones 
               
               
                   
                 and aminoesers. Otherwise, it also contains 8 amino acids 
               
               
                   
                 including aspartic acid. 
               
               
                 ORIGINAL 
                 The decoctant of  Buodia rutaecarpa  show stronger inhibitive 
               
               
                 RECORDED 
                 effects on  V. choleroae . It also shows certain degree of 
               
               
                 PHARMACOLOGICAL 
                 inhibitive effects on  Pseudomonas aeruginosa , 
               
               
                 EFFECTS 
                   Staphylococcus aureaus  and some common disease-causing 
               
               
                   
                 fungus. (W-F, Dam etc. Chinese medicine 1952, 38(4): 315) 
               
               
                   
                 The evodiamine show anti-viruses effect on rats which 
               
               
                   
                 infected by Columbia SK viruses. (Chou SC. CA, 1967, 
               
               
                   
                 67: 61622j) The water, ethanol and ether decoctants of 
               
               
                   
                   Buodia rutaecarpa  can kill pig ascarids, earth worms and 
               
               
                   
                 leeches in vitro. (Y-R, Wu etc. Chinese medicine, 1948, 
               
               
                   
                 34(10): 437) 
               
               
                   
               
             
          
         
       
     
         [0047]    
       
         
               
             
               
               
               
               
             
               
               
             
           
               
                   
               
               
                   
               
               
                 NO: 4 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 HERBAL NAME 
                 Baibu 
                 OFFICIAL NAME 
                 
                   Stemona tuberose 
                 
               
               
                   
                   
                   
                 Lour 
               
               
                   
               
             
          
           
               
                 REFERENCE BASIS 
                 China Pharmacopoeia Volume VIII pp 189 (China 
               
               
                   
                 Sheng-Ghai scientific technology publication) 
               
               
                 GROWING DISTRICT 
                   Stemona tuberose  grows in southeast province of China, 
               
               
                 &amp; COLLECT SEASON 
                 Hu-nan, Hu-peh and Si-chuan. Collect them after 3 years of 
               
               
                 &amp; PARTS 
                 planting. Remove tubers after wilting of upper parts of shoot 
               
               
                   
                 before spring. Remove roots and boiling in hot water to well 
               
               
                   
                 done then drying or baking them. 
               
               
                 PREPARATION &amp; 
                 Take 12 kg of  Sophora flavescens  and soak in water for 3 
               
               
                 EXTRACTION 
                 hours. Collect percolate for 2 times then evaporate the 
               
               
                 PROCESS &amp; METHOD 
                 residue water to get 5 liters of fluid extract. Adding 2 kg 
               
               
                   
                   Dioscorea collettii  of powder and stir well. Drying it in 60° C. 
               
               
                   
                 then pulverized by passing #120 sieve to make powder. 
               
               
                   
                 Collect and store them for later use. 
               
               
                 EFFECTIVE 
                 The  Stemona tuberose  contains 13 alkaloids including 
               
               
                 INGREDIENT 
                 syemonine, tuberostemonine and isotuberostemonine. It also 
               
               
                   
                 contains sugar, fats, proteins, ash and 6 different acids 
               
               
                   
                 including oxalic acid. 
               
               
                 ORIGINAL 
                 1. Anti-microbe. 
               
               
                 RECORDED 
                 2. Anti-parasite: In vitro tests show that 50% solution can 
               
               
                 PHARMACOLOGICAL 
                 kill pin worm in 20 hours. The tuberostemonine can paralyze 
               
               
                 EFFECTS 
                 Ascarid. 
               
               
                   
                 3. Insect annihilation effects: The  Stemona tuberose  water 
               
               
                   
                 solution and alcohol solution has effects on lice and cause 
               
               
                   
                 eggs of lice not hatch. It also show contact annihilation 
               
               
                   
                 effect on 10 or more pests including maggot, larva of 
               
               
                   
                 mosquito, bed bug, aphids, larva of  Chilo simples  and spider. 
               
               
                   
               
             
          
         
       
     
         [0048]    
       
         
               
             
               
               
               
               
             
               
               
             
           
               
                   
               
               
                   
               
               
                 NO: 5 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 HERBAL NAME 
                 Luodishenggen 
                 OFFICIAL NAME 
                 
                   Bryophyllum 
                 
               
               
                   
                   
                   
                   pinnatum  (L. f.) 
               
               
                   
               
             
          
           
               
                 REFERENCE BASIS 
                 China Pharmacopoeia Volume III pp 752 (China Sheng-Ghai 
               
               
                   
                 scientific technology publication) 
               
               
                 GROWING DISTRICT 
                 It grows in Fu-jann, Taiwan, Kwang-tong, Kwang-si and 
               
               
                 &amp; COLLECT SEASON 
                 Yun-nan. 
               
               
                 &amp; PARTS 
               
               
                 PREPARATION &amp; 
                 Take 36 kg of fresh leaves and stems of  Bryophyllum   
               
               
                 EXTRACTION 
                   pinnatum  and put them into colloid mill for grinding. Collect 
               
               
                 PROCESS &amp; METHOD 
                 percolate for 3 times and place it on the steel plates them 
               
               
                   
                 transfer to “low temperature gas chilled dryer” then 
               
               
                   
                 evaporate the residue water to get 5 liters of concentrate 
               
               
                   
                 solution. Adding 2 kg of  Sophora flavescens  powder and stir 
               
               
                   
                 well. Drying it in low temperature then pulverized by 
               
               
                   
                 passing #120 sieves to make powder. Collect and store them 
               
               
                   
                 for later use. 
               
               
                 EFFECTIVE 
                 The  Bryophyllum pinnatum  contains cis-aconitic acid, 
               
               
                 INGREDIENT 
                 ascorbic acid, eugenic acid, caffeic acid, p-hydroxy benzoic 
               
               
                   
                 acids and many organic acids. It also contains quercitin, 
               
               
                   
                 kafmferol, avicularin, 18 α - oleanane , ψ -taraxasterol, 
               
               
                   
                 β-Amyrin acetate, 24-ethyl-25 hydroxy cholesterol, α- 
               
               
                   
                 Amyrin, β-Amyrin,  Bryophyllum pinnatum  steroids, 
               
               
                   
                 ketones, alkene ketones and alcohols. 
               
               
                 ORIGINAL 
                 The juice from leaves of  Bryophyllum pinnatum  in 5%(v/v0) 
               
               
                 RECORDED 
                 concentration show broad spectrum of bactericidal effects on 
               
               
                 PHARMACOLOGICAL 
                 Gram&#39;s positive and Gram&#39;s negative bacteria such as 
               
               
                 EFFECTS 
                   Staphyllococcus aureaus ,  Streptococcus pyrogens ,  S.   
               
               
                   
                   faecalis ,  E. Coli ,  Proteus ,  Klebsiells ,  Shigella ,  Salmonella , 
               
               
                   
                   Serratia marcescens  and  Pseudomonas aeruginosa . They 
               
               
                   
                 including many resistant bacteria separated from clinical. 
               
               
                   
                 (Obaselki E. Afr J Med Sci, 1985, 14(3-4); 199) 
               
               
                   
               
             
          
         
       
     
         [0049]    
       
         
               
             
               
               
               
               
             
               
               
             
           
               
                   
               
               
                   
               
               
                 NO: 6 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 HERBAL NAME 
                 Kushen 
                 OFFICIAL NAME 
                 
                   Sophora 
                 
               
               
                   
                   
                   
                 
                   flavescens 
                 
               
               
                   
                   
                   
                 Ait 
               
               
                   
               
             
          
           
               
                 REFERENCE BASIS 
                 China Pharmacopoeia Volume IV pp 634 (China Sheng-Ghai 
               
               
                   
                 scientific technology publication) 
               
               
                 GROWING DISTRICT 
                 Grow in everywhere in China. Take all plants in September 
               
               
                 &amp; COLLECT SEASON 
                 to October and remove the upper part, base of stem and 
               
               
                 &amp; PARTS 
                 fibrous roots. Cut fibrous roots into single strand. Wash out 
               
               
                   
                 dirt then dry or bake it. 
               
               
                 PREPARATION &amp; 
                 Take 12 kg of  Sophora flavescens  and soak in water for 3 
               
               
                 EXTRACTION 
                 hours. Collect percolate for 2 times then evaporate the 
               
               
                 PROCESS &amp; METHOD 
                 residue water to get 5 liters of fluid extract. Adding 2 kg 
               
               
                   
                   Dioscorea collettii  of powder and stir well. Drying it in 60° C. 
               
               
                   
                 then pulverized by passing #120 sieve to make powder. 
               
               
                   
                 Collect and store them for later use. 
               
               
                 EFFECTIVE 
                 The roots contain 13 alkaloids including matrine and many 
               
               
                 INGREDIENT 
                 flavonoids mixtures. Otherwise, the roots contain tri-terpene 
               
               
                   
                 saponin, soy saponin and kurarinone. 
               
               
                 ORIGINAL 
                 The in vitro experiments show that 1% matrine has apparent 
               
               
                 RECORDED 
                 bacteria inhibitive effects on  Shigella ,  E. Coli ,  Proteus , 
               
               
                 PHARMACOLOGICAL 
                   B-streptococcus  and  Staphylococcus aureaus . The total 
               
               
                 EFFECTS 
                 flavones of  Sophora flavescens  and their ethanol extract 
               
               
                   
                 have annihilation or growth inhibitive effects on  Giardia   
               
               
                   
                   lamblia  Stiles in vitro. 
               
               
                 PHARMACOLOGICAL 
                 It can use specific alkaloids and saponins to achieve goals of 
               
               
                 APPLICATIONS, 
                 annihilation pests and bacteria. The inventor observed 20 
               
               
                 PURPOSES 
                 concentrated Chinese medicine drugs powder all using 
               
               
                   
                 starch as adjuvant for 20 years and found that the  Sophora   
               
               
                   
                   flavescens  did not eat into by worms and not change its 
               
               
                   
                 quality. The package still remains intact. It shows the effect 
               
               
                   
                 of germicide. 
               
               
                   
               
             
          
         
       
     
         [0050]    
       
         
               
             
               
               
               
               
             
               
               
             
           
               
                   
               
               
                   
               
               
                 NO: 7 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 NAME 
                 Zisuye 
                 OFFICIAL NAME 
                 
                   Perilla frutescens 
                 
               
               
                   
                   
                   
                 (L.) Britt. 
               
               
                   
               
             
          
           
               
                 REFERENCE BASIS 
                 China Pharmacopoeia Volume VII pp 115 (China 
               
               
                   
                 Sheng-Ghai scientific technology publication) 
               
               
                 GROWING DISTRICT 
                   Perilla frutescens  grows best in Kwang-tong, Kwang-si and 
               
               
                 &amp; COLLECT SEASON 
                 Hu-peh. Collect them in July or August as leaves and twigs 
               
               
                 &amp; PARTS 
                 prosper. Remove leaves after spread on the ground or place 
               
               
                   
                 in well ventilated areas for drying. 
               
               
                 PREPARATION &amp; 
                 Take 12 kg leaves of  Perilla frutescens  and pressed firmly in 
               
               
                 EXTRACTION 
                 the distillation flask. By using distillation method to get 
               
               
                 PROCESS &amp; METHOD 
                 volatile oil. Collect and store them for later use. 
               
               
                 EFFECTIVE 
                 The  Perilla frutescens  contains perillaldehyde, limonene, β- 
               
               
                 INGREDIENT 
                 caryophllene, and linalool. It also contains perilla-alcohol- 
               
               
                   
                 β -d-pyranoglucoside. 
               
               
                 ORIGINAL 
                 The distilled fractions of  Perilla frutescens  have broad 
               
               
                 RECORDED 
                 spectrum of anti-bacteria effects. The main effective 
               
               
                 PHARMACOLOGICAL 
                 ingredient is perillaldehyde. The perillaldehyde and tadeonal 
               
               
                 EFFECTS 
                 show apparently cooperative effect in anti-fungal effects. 
               
               
                   
                 (Kang R. et al. CA, 1912, 117: 2087762) The perillaldehyde 
               
               
                   
                 and tadeonal show anti-fungal effects on  Trichophyton   
               
               
                   
                   rubrum ,  Trichophyton mentagrophytes ,  Microsprum   
               
               
                   
                   gypseum ,  Microsprum canis . (Pharmacognology (Japan), 
               
               
                   
                 1984, 38(1): 127). The oil from leaves of  Perilla frutescens   
               
               
                   
                 shows apparently inhibitive effects on  Aspergillus niger , 
               
               
                   
                   Penicillium  and yeast. (C-Y, Chang et al., China Chinese 
               
               
                   
                 medicine, 1990, 15(2): 31) The decoctant of  Perilla   
               
               
                   
                   frutescens  (1:20) show inhibitive effect on ECHO11 viruses 
               
               
                   
                 in vitro. (New medicine, 1973,(12): 38) 
               
               
                   
               
             
          
         
       
     
         [0051]    
       
         
               
             
               
               
               
               
             
               
               
             
           
               
                   
               
               
                   
               
               
                 NO: 8 Emulsifying oil agents 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 NAME 
                 Guanhuoxiang 
                 OFFICIAL NAME 
                 
                   Pogostemon Cablin 
                 
               
               
                   
                   
                   
                 ( Blanco )  Benth   
               
               
                   
               
             
          
           
               
                 REFERENCE BASIS 
                 China Pharmacopoeia Volume VII pp 130 (China 
               
               
                   
                 Sheng-Ghai scientific technology publication) 
               
               
                 GROWING DISTRICT 
                   Pogostemon Cablin  grows in Kwang-tong, Hai-nan and 
               
               
                 &amp; COLLECT SEASON 
                 Taiwan. Harvest them in August to November or divided 
               
               
                 &amp; PARTS 
                 into several times. Spread it under the sun till the leaves 
               
               
                   
                 shrink then transfer to interior and stack in layers. Put hay 
               
               
                   
                 and woods on it and press firmly. Let it dripping for one 
               
               
                   
                 night then the twigs and leaves are getting yellow. Spread it 
               
               
                   
                 under the sun in next day. Repeat these procedures until 
               
               
                   
                 complete dry reached. 
               
               
                 PREPARATION &amp; 
                 Take 12 kg of  Pogostemon Cablin  and pressed firmly in the 
               
               
                 EXTRACTION 
                 distillation flask. By using distillation method to get volatile 
               
               
                 PROCESS &amp; METHOD 
                 oil. Collect and store them for later use. 
               
               
                 EFFECTIVE 
                 The stems and leaves of  Pogostemon Cablin  contain volatile 
               
               
                 INGREDIENT 
                 oil. Its mainly pathchoulo alcohol, pathchoulo diol, α- 
               
               
                   
                 quaiene, δ - quaiene, α -bulnesene, α -pogostene and 
               
               
                   
                 pogostone. It also contains β - quaiene, β -elemene, β- 
               
               
                   
                 caryophllene, δ -cadinene, and patchoulodiol. 
               
               
                 ORIGINAL 
                 The pogostone show apparently inhibitive effects on fungi 
               
               
                 RECORDED 
                 such as  C. albicans ,  Cryptococcus neoformans  and  Rhizopus   
               
               
                 PHARMACOLOGICAL 
                   stolonifer . It shows certain degree of inhibitive effect on type 
               
               
                 EFFECTS 
                 A hemolytic  Streptococcus . (J-S, Yang, Science report, 1977, 
               
               
                   
                 22(7): 318) The pogostone can inhibit the growth of fungi 
               
               
                   
                 such as  Penicillum . (T-S, Kung, Chinese herbs, 1984, 
               
               
                   
                 15(3): 16) 
               
               
                   
               
             
          
         
       
     
         [0052]    
       
         
               
             
               
               
               
               
             
               
               
             
           
               
                   
               
               
                   
               
               
                 NO: 9 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 NAME 
                 Tanxiang 
                 OFFICIAL NAME 
                   Santalum album  L. 
               
               
                   
               
             
          
           
               
                 REFERENCE BASIS 
                 China Pharmacopoeia Volume II pp 592 (China Sheng-Ghai 
               
               
                   
                 scientific technology publication) 
               
               
                 GROWING DISTRICT 
                   Santalum album  is imported from India, Indonesia and 
               
               
                 &amp; COLLECT SEASON 
                 Malaysia. 
               
               
                 &amp; PARTS 
               
               
                 PREPARATION &amp; 
                 Take natural yellow colored old blocks of  Santalum album   
               
               
                 EXTRACTION 
                 from the market. Shave them into small pieces or chop them 
               
               
                 PROCESS &amp; METHOD 
                 into crude powder and pressed firmly in the distillation flask. 
               
               
                   
                 By using distillation method to get volatile oil. Collect and 
               
               
                   
                 store them for later use. 
               
               
                 EFFECTIVE 
                 The core wood of  Santalum album  contains volatile oil 
               
               
                 INGREDIENT 
                 which is called white sandalwood oil. It&#39;s content is about 
               
               
                   
                 1.5% to 5%. The main ingredients are sesquiterpene 
               
               
                   
                 compounds such as α - santol and β -santol. 
               
               
                 ORIGINAL 
                 The α - santol and β -santol show stronger anti-bacteria 
               
               
                 RECORDED 
                 effects. The bacteria inhibition concentration of santol in 
               
               
                 PHARMACOLOGICAL 
                 vitro is 1:64000 to 1:128000. It is also effective to  Shigella   
               
               
                 EFFECTS 
                 species and shows inhibitive effect on  M. avium  at 
               
               
                   
                 concentration 1:32000. (Okazakik, et al. CA. 1953, 
               
               
                   
                 47: 7167 g)