Abstract:
An array of nucleic acid probes is described for identifying and/or characterizing a microorganism. Methods are also described for detecting the presence of a microorganism in a sample, as well as determining its pathotype, using the array. Methods of assessing related infection and disease in a subject using the array are also described. Methods that characterize complex microbial communities using the array are also described.

Description:
CROSS-REFERENCE TO RELATED APPLICATIONS  
       [0001]     This application claim priority on prior application Ser. No. 60/453,288 filed Feb. 11, 2004, the entire content of which is hereby incorporated by reference. 
     
    
     TECHNICAL FIELD  
       [0002]     The present invention relates to a DNA array plate and uses thereof, and more particularly to an array for detecting or pathotyping a microorganism and uses thereof. The present invention also relates to the use of a DNA array plate to characterize complex microbial mixtures and microbial communities. The present invention also demonstrates the use of a DNA array plate to determine the presence of antibiotic resistance genes in complex microbial mixtures and communities.  
       BACKGROUND OF THE INVENTION  
       [0003]     Single species microbial products and complex microbial mixtures containing live microorganisms (consortia) are sold commercially and used by the general public and commercial biotechnology users. From a regulatory viewpoint, there are no easy methods to characterize these consortia in terms of the taxonomy and function of the microorganisms present. The presence or absence of microbial pathogens in these products is also difficult to assess. The presence of molecular and physiological mechanisms for antibiotic or metal resistance, which are of concern in terms of spreading these traits within the bacterial pathogens to which humans may be exposed, is also difficult to assess in these microbial products. The same problems are also found when analyzing microbial populations from biotechnology processes, water, air, soil or food samples. With reference to commercial microbial products characterization and quality control, the stability of microorganisms and their reproducibility between different batches or lots from the same supplier over a period of months to years is also difficult to assess with current methods.  
         [0004]     Presently, basic microbiology methods relying on culturing methods followed by microscopic, morphological and biochemical tests are the principal approach to characterize the microorganism(s). Once the microorganisms are grown, methods such as fatty acid analysis by gas chromatography and mass spectrometry can be applied to identify the microbial species present. Additional microbiology methods can be used to study phenotypic traits of cultured microorganisms such antibiotic resistance, metal resistance, or catabolic properties. Newer molecular methods such as the Polymerase Chain Reaction (PCR) method can be used to detect certain specific genes of interest, for instance those genes coding for bacterial toxins, antibiotic resistance genes or catabolic properties (e.g. lipases, proteases, cellulases) that are suspected of being present in a given microbial sample under study. Electrophoresis methods like such as Denaturing Gradient Gel Electrophoresis (DGGE) can be used to provide genetic fingerprints and estimates of the microbial diversity present in a given consortium.  
         [0005]     The methods are generally complex, time-consuming and applicable only to suspected pathogens or genes of specific concern in a given sample. They are also notoriously inadequate for characterizing microbial diversity since perhaps as many as 95-99% of microorganisms in complex microbial communities cannot be cultured with existing media and methods used in laboratories. Culture methods show inherent variability in their ability to grow bacteria that have been stored for weeks or months prior to use. Also, viable but nonculturable (VBNC) microorganisms cannot be grown and detected. Electrophoretic methods such as DGGE are labor-intensive and require skilled research personnel to achieve reproducible results. They also only provide a rough genetic fingerprint of microbial diversity in a consortia, and another overly labour-intensive DNA sequencing step is required to learn more about the taxonomic composition and potential presence of pathogens in complex consortia. While PCR methods can be applied to detecting specific microorganisms or genes of interest in consortia, independent PCR tests for many different microorganisms and genes can quickly become overly complicated and cost prohibitive.  
         [0006]     It would be highly desirable to be provided with a DNA array capable of characterizing and even discriminating between multiple microorganism species in a sample, all in one assay.  
       SUMMARY OF THE INVENTION  
       [0007]     One aim of the present invention is to provide a DNA array capable of characterizing and even discriminating between multiple microorganism species in a sample, all in one assay.  
         [0008]     Another aim of the present invention is to provide a method for characterizing numerous microorganism in a same assay.  
         [0009]     According to one aspect of the invention, a DNA microarray is provided including immobilized probes capable of providing a genetic fingerprint of a single species or of a consortia of microorganisms of interest in diverse water, soil, food, environmental and clinical samples, and recognizing specifically and simultaneously the presence therein of a plurality of gene classes such as: 
        a) Taxonomically significant genes such as 16S genes, heat shock proteins, RNA polymerase, DNA gyrase. Through a judicious selection of probes, information can be obtained on the presence or absence of diverse and similar microorganisms of environmental or human health relevance in a parallel, simultaneous fashion.     b) Functionally significant genes such as lipases, cellulases or proteases. As an example, a drain-cleaning microbial consortium lacking the stable presence of known lipase genes may be suspected of poor or inconsistent efficacy.     c) Genes of clinical interest to humans, wild animals, pets, livestock, insects, plants, biocontrol agents, such as antibiotic resistance genes,     d) Genes coding for known virulence factors, growth factors and toxins, to protect against inadvertent or deliberate contamination of a microbial product or process by pathogenic agents.     e) Any other gene of interest such as genes coding for specific proteins or macromolecules, cell components, waste products and antimicrobial agents.        
 
         [0015]     According to yet another aspect of the invention, a method for providing a genetic fingerprint of a single species or of consortia of microorganisms in diverse water, soil, food, environmental and clinical samples, and recognizing specifically and simultaneously the presence therein of a plurality of gene classes, is provided comprising, a) extracting the total DNA from a microbial sample, b) labeling the sample with a detectable label and c) applying the labeled sample to a DNA microarray, wherein specific hybridization will occur with the relevant probes or oligonucleotides printed on the DNA microarray, d) reading the microarray with means appropriate to detect the detectable label (whether radioactive, non-radioactive, fluorescent, calorimetric, immunological, enzymatic, spectrophotometric or simply by visual detection with the unaided eye or through a microscope), to provide information simultaneously on all the types of probes mentioned above as to whether the sample contains or not sequences complementary to the probes printed on the DNA microarray.  
         [0016]     According to a further aspect of the invention, there is also provided a microarray comprising thereon cpn60 probes and other useful probes such as 16S, antibiotic resistance, virulence genes, functional genes, for characterization of commercial microbial consortia. While the use of 16S is universal for species identification, its closely conserved nature leads to difficulties when closely related species are considered, such as  Bacillus megaterium  and  Bacillus licheniformis  (8% distance between the 16S gene sequences). Under those circumstances, the use of a more rapidly evolving gene such as cpn60 gives more differentiation.  
         [0017]     According to yet a further aspect of the invention, the use of the microarrays according to the invention in the characterization of microbial communities in food microbiology, soil microbiology, water quality analysis, bio-terrorism detection, microbial air quality and similar applications, is also provided.  
         [0018]     In accordance with the present invention, thereis also provided an array which comprises: 
        a) a substrate; and     b) a plurality of nucleic acid probes specifically and simultaneously recognizing the presence of a plurality of different genes, each of said probes being bound to said substrate at a discrete location; said plurality of probes comprising a first probe for detecting a first gene of a species of a microorganism and at least another probe for detecting at least one other gene of said species or of a different species of a microorganism.        
 
         [0021]     Preferably, the array comprises at least two different probes specific for a single gene. The array may have a subarray containing said at least two probes at adjacent discrete locations on said substrate.  
         [0022]     In one embodiment of the invention, the first probe as described above is specific for a virulence gene or a fragment thereof or a sequence substantially identical thereto, and the at least one other probe is specific for an antibiotic resistance gene. Alternatively, the first probe can be specific for a variant of a virulence gene or a fragment thereof or a sequence substantially identical thereto, and the at least one other probe is specific for an antibiotic resistance gene, the first probe allowing detection of different types and/or species of microorganism.  
         [0023]     The microorganism can be a bacterium, and more particularly one of the family Enterobacteriaceae, such as  E. coli.    
         [0024]     In a further embodiment, the virulence gene encodes a polypeptide of a class of proteins selected from the group consisting of toxins, adhesion factors, secretory system proteins, capsule antigens, somatic antigens, flagellar antigens, invasins, autotransporter proteins, and aerobactin system proteins. In another embodiment of the invention, the different genes can be selected from the group consisting of Tem, Shv, oxa-1, oxa-7, pse-4, ctx-m, ant(3″)-Ia (aadA1), ant(2″)-Ia (aadB)b, aac(3)-IIa (aacC2), aac(3)-IV, aph(3′)-Ia (aphA1), aph(3′)-IIa (aphA2), tet(A), tet(B), tet(C), tet(D), tet(E), tet(Y), catI, catII, catIII, floR, dhfrI, dhfrV, dhfrVII, dhfrIX, dhfrXIII, dhfrXV, suII, suIII, integron classe 1 3′-CS, vat, vatC, vatD, vatE, vga, vgb, and vgbB.  
         [0025]     Preferably, in one further embodiment of the invention, the plurality of nucleic acid probes are sequences selected from the group consisting of SEQ ID NO:1 to SEQ ID NO:64, or a fragment thereof, or a sequence having at least 50% identity, preferably at least 70% identity, more preferably having 80% identity and most preferably having 90% identity with said sequences.  
         [0026]     The plurality of different genes can also be selected from the group consisting of 16S gene, genes encoding heat shock proteins, gene encoding RNA polymerase, gene encoding DNA gyrase, gene encoding a lipase, gene encoding a cellulose, gene encoding a protease, genes of clinical interest, gene encoding virulence factor, gene encoding growth factor, and gene encoding a toxin.  
         [0027]     In a still further embodiment of the invention, the first probe is specific for a 16S gene or a fragment thereof or a sequence substantially identical thereto, and the at least one other probe is specific for cpn60 gene.  
         [0028]     Also, in accordance with the present invention, there is provided a method of detecting the presence of a microorganism in a sample. The method comprises the steps of: 
        a) contacting an array as described above with a sample nucleic acid of said sample; and     b) detecting association of said sample nucleic acid to a probe on said array;     wherein association of said sample nucleic acid with said probe is indicative that said sample comprises a microorganism from which the nucleic acid sequence of said probe is derived.        
 
         [0032]     The method may also comprise optionally a step of extraction of the sample nucleic acid from said sample prior to contacting said sample nucleic acid with said array.  
         [0033]     The sample can be an environmental sample (such as from water, air or soil), a biological sample (such as blood, urine, amniotic fluid, feces, tissues, cells, cell cultures and biological secretions, excretions or discharge) or a food sample. Alternatively, the biological sample can be a tissue, body fluid, secretion or excretion from a subject.  
         [0034]     In accordance with a further embodiment of the invention, there is also provided a method for determining a pathotype of a species of a microorganism in a sample, said method comprising the steps of: 
        a) contacting the array as defined previously with a sample nucleic acid of said sample; and     b) detecting association of said sample nucleic acid to a probe on said array;     wherein association of said sample nucleic acid with said probe is indicative that said sample having a pathotype from which the nucleic acid sequence of said probe is derived.        
 
         [0038]     Still in accordance with the present invention, there is also provided a method for diagnosing an infection by a microorganism in a subject, said method comprising the steps of: 
        a) contacting the array as defined previously with a sample nucleic acid of said sample; and     b) detecting association of said sample nucleic acid to a probe on said array;     wherein association of said sample nucleic acid with said probe is indicative that said sample has been infected by a microorganism from which the nucleic acid sequence of said probe is derived.        
 
         [0042]     According to the present invention, there is also provided a kit comprising the array as described above together with instructions for use thereof, such as uses for 
        (a) detecting the presence of a microorganism in a sample;     (b) determining the pathotype of a microorganism in a sample;     (c) diagnosing an infection by a microorganism in a subject; or     (d) diagnosing a condition related to infection by a microorganism, in a subject.        
 
         [0047]     (e) characterizing a microbial complex sample or microbial community on a one-time basis 
        (f) following the evolution over time of a microbial complex sample or microbial community. This may include comparison between different batches of commercial products based on complex microbial samples, comparison between similar products from different suppliers and monitoring the bacterial composition of commercial products over storage time.        
 
       INDUSTRIAL APPLICABILITY  
       [0049]     The method proposed is generally applicable to any sample requiring microbiological analysis, such as: 
        i. single microbial species, clinical samples, commercial microbial consortia and communities of microorganisms from air, water and soil;     ii. food, food samples, food ingredients, livestock and pet food and the raw ingredients for making such foods;     iii. cosmetics, medications, pharmaceutical products and the raw ingredients to make such products;     iv. wastewater samples, potable water, raw water, surface water, groundwater, water treatment facilities, sewage samples;     v. bioreactor samples;     vi. human and veterinary clinical samples such as fecal or urine samples, animal tissue samples, rumen or stomach samples;     vii. plants, seeds, roots, plant surfaces, plant transplants, horticultural samples, nutrient recycling samples, plant rhizosphere, plant rhizoplane;     viii. environmental surfaces;     ix. samples from the manufacture or production of biological products, microorganisms, insects, protozoa;     x. goods produced in controlled atmosphere such as medical devices or electronics components; and     xi. any other samples where microorganisms can be detected and sampled.        
 
         [0061]     An initial application of the invention resides in assisting biotechnology companies to meet notification requirements for consortia products under the Canadian Environmental Protection Act within Environment Canada. Commercial application may also be found within contract research or quality control laboratories. This invention could also be used for detection and/or identification of biological warfare agents camouflaged as commercial products. The invention can also be applied to any type of single microbial species or complex microbial consortium or mixture, within detection limits and given the design of suitable probes for each particular consortium. Therefore, companies that specialize in the detection and identification of microorganisms may also be interested. Also, companies involved and microbiological aspects of environmental, air quality and food monitoring, whether in consulting, R&amp;D, quality control or research are expected to show interest worldwide. Basic research laboratories throughout the world will also be interested in the present invention.  
         [0062]     For the purpose of the present invention the following terms are defined below.  
         [0063]     The term “probe” is used herein interchangeably with amplicon and oligonucleotide of at least 18 or more nucleotides in length and preferably of at least 70 nucleotides in length.  
         [0064]     The term “array” used herein is interchangeably used with the expression “array plate” or “DNA chip”.  
         [0065]     The term “specific for” when used to set a probe in relation to a gene is intended to mean that said probe recognizes only the gene for which the probe is specific. Of course, a skilled person will appreciate that probes with silent substitutions, deletions or additions may as well be used in accordance with the present invention. 
     
    
     BRIEF DESCRIPTION OF THE DRAWINGS  
       [0066]      FIG. 1A  illustrates a number of bacteria and antibiotic resistant bacteria present in commercial consortia, grown in LB media alone or in LB media containing Ampicillin, Chloramphenicol, Kanamycin, Streptomycin or Tetracycline at 25 or 50 μg/mL concentration;  
         [0067]      FIG. 1B  illustrates the direct detection of antibiotic resistance genes in genomic DNA extracted from a commercial consortium, the sequences of the probes detecting antibiotic resistance are found in Table 1;  
         [0068]      FIG. 2  illustrates the detection results obtained on Biozyme 5000 commercial product, wherein solid yellow box represents an expected signal, the dashed red box represents samples known to cross react and dashed orange box represents a possible signal, the content being reported in Table 2;  
         [0069]      FIGS. 3A  to  3 C illustrate the content (3A) printed on the microarray plates and the discriminating power of cpn60 probes between B38  B. megaterium  (500 ng of DNA) (3B) and B16  B. licheniformis  (500 ng of DNA) (3C);  
         [0070]      FIGS. 4A and 4B  illustrate the Key for the amplicon microarray used to illustrate the superior discriminating power of cpn60 genes between closely related species (4A) and the detection results (4B) of  Amphibacillus xylanus  using a combination of cpn60 and 16S probes;  
         [0071]      FIG. 5  illustrates the detection results of  Bacillus amyloliquefaciens  using a combination of cpn60 and 16S probes as set out in Table 3, using the key illustrate in  FIG. 4A ;  
         [0072]      FIG. 6  illustrates the detection results of  Halobacillus halophilus  using a combination of cpn60 and 16S probes as set out in Table 3, using the key illustrate in  FIG. 4A ; and  
         [0073]      FIG. 7  illustrates the detection results of  Virgibacillus pantothenticus  using a combination of cpn60 and 16S probes as set out in Table 3, using the key illustrate in  FIG. 4A . 
     
    
     DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENT  
       [0074]     One of the concerns associated with the increasing occurrence of highly antibiotic resistant pathogenic bacteria in hospitals has been to find ways to slow the circulation of the resistance genes. In this context, increased use of microbial biotechnology products, particularly in consumer household environments, may be a concern if these products are found to contain medically significant antibiotic resistance genes. As shown in  FIG. 1 , commercially available microbial consortia in Canada have been found to contain high counts of antibiotic resistant bacteria.  
         [0075]     To address these and other antibiotic resistance genes that may be present in commercial consortia, the inventors have developed antibiotic resistance gene probes for the consortium analysis microarray. The current design uses both oligonucleotides (18 to 70 bases) and amplicons as probes, to obtain the best trade-offs in sensitivity versus specicifity.  
         [0076]     Presently, prokaryotic taxonomy is based, in part, upon sequence differences in the gene encoding 16S ribosomal RNA. This ordering makes sense for the most part and allows the discrimination of general taxonomic groups. However, within a narrow taxonomic group such as within a particular genus, 16S becomes less reliable as a taxonomic discriminator. Other genes, such as cpn60, a gene that encodes a 60 kDa chaperonin found in all bacteria, can also be used to delineate taxonomic lines due to its greater sequence diversity than 16S. A DNA microarray was printed with a combination of amplicon probes containing the sequences of 16S and cpn60 from a number of  Bacillus  and  Bacillus -like species. The array was then hybridized with fluorescently-labelled amplicons of 16S and cpn60 amplified from different species that were represented on the microarray. The aim of the current work was to: 1) ascertain the validity of using such a dual taxonomic factor approach for discriminating between closely-related  Bacillus  species, and 2) determine whether the level of target discrimination required was achievable using DNA microarrays. The results confirm the complementarity that exists through the concomitant use of both taxonomic factors, and the parallel processing inherent in DNA microarrays, makes it a powerful tool to rapidly identify bacterial isolates at the species level.  
         [0077]     The temperature at which a hybridization is carried out appears to be a major factor in achieving specificity. The 16S and cpn60 amplicons are of similar length (520-550 bp), but the 16S amplicons have a significantly higher GC content (57%) and melting temperature than the cpn60 amplicons (44%). This makes simultaneous hybridizations of the two amplicons on the same array less than optimal. However, by hybridizing at 55° C., a temperature between the optimum for each type of amplicon, signal discrimination for the cpn60 and 16S probes was obtained.  
         [0078]     Due to the relatively small differences in sequence amongst the 16S probes printed on the array, some cross hybridization is expected. However, cross hybridizing signal should be proportional to the sequence similarity between the probe and target. The role of the 16S probes was to discriminate between different genera of bacteria, such as Halobacillus and  Bacillus , while the cpn60, due to its greater variation, could discriminate at the species level.  
         [0079]     The concept of a dual backbone microarray assay for the taxonomic discrimination of closely related bacteria was proven to work with amplicon hybridizations. Further work will examine whether this is valid with genomic DNA hybridizations.  
         [0080]     The following tables 1 to 3 give a summary of the current status of probe development:  
                                                                                                                 TABLE 1                           70-mer oligonucleotide probes for commonly           encountered antibiotic resistance genes in bacteria                    Length               BLAST result,                           of           G + C   (mm =   Accession       Gene   Oligo probe (5′to 3′)   sequence   Position   Tm   content   mismatches)   number   oligo name                    Gram-           negative            tem   AAA GTT CTG CTA TGT GGC GCG   70   8674-   80.4   57.1   tem(X)   AF307748   70-tem8674               GTA TTA TCC CGT GTT GAC GCC       8605           GGG CAA GAG CAA CTC GGT CGC           CGC ATA C (SEQ ID NO:1)               shv   CTC AAG CGG CTG CGG GCT GGC   70   86-17   83.7   64.3   shv(X)   AF148850   70-shv86           GTG TAC CGC CAG CGG CAG GGT           GGC TAA CAG GGA GAT AAT ACA           CAG GCG A (SEQ ID NO:2)               oxa-1   AAA CAA CCT TCA GTT CCT TCA   70   256-187   74.3   44.3   oxa-1   AJ238349   70-           AAT AAT GGA GAT GCG ACA GTA                           oxa(1)256           GAG ATA TCT GTT GAT GCA CTG           GCG CTG C (SEQ ID NO:3)               oxa-7   GTA GCG CAG GCT AAT TTA CTG   70   295-226   75.2   45.7   oxa-13, oxa-19,   X75562   70           CTA CTT TTA CAA AGC ACG AAA                   oxa-14, pse-2,   oxa(7)295           ACA CCA TTG ACG GCT TCG GCA                   oxa-10, oxa-17,           GAG AAC T (SEQ ID NO:4)                   oxa-16, oxa-7               pse-4   CGC TGA TTG CCA TTG TAA TCC   70   348-79   72.3   41.4   pse-4, pse-5,   J05162   70-           CAA TAT TCT CCA TTT TGA GTA                   carb-6, pse-1       pse(4)348           TCA AGA ACG GAA ACA CCT ATA           CGA GCA G (SEQ ID NO:5)               ctx-m   ATA CAG CGG CAC ACT TCC TAA   70   143-74   80.3   55.7   ctx-m-1, ctx-m-3,   X92506   70-ctx143           CAA CAG CGT GAC GGT TGC CGT                   ctx-m-28, ctx-m-,           CGC CAT CAG CGT GAA CTG ACG                   27, ctx-m-22,           CAG TGA (SEQ ID NO:6)                   ctx-m-27, ctx-                               m-15               ant-   ATG ATG TCG TCG TGC ACA ACA   70   290-221   79.2   55.7   aadA1, aadA2   X12870   70-   70-       (3“)-la   ATG GTG ACT TCT ACA GCG CGG                           aadA(1)290       (andA1)   AGA ATC TCG CTC TCT CCA GGG           GAA GCC G (SEQ ID NO:7)               ant-   CCC GAG TGA GGT GCA TGC GAG   70   1778-   79.1   55.7   aadB   M86913   70-       (2“)-la   CCT GTA GGA CTC TAT GTG CTT       1709                   aadB1778       (aadB) b     TGT AGG CCA GTC CAC TGG TGG           TAC TTC A (SEQ ID NO:8)               aac(3)-   CAC CGG TTT GGA CTC CGA GTT   70   200-131   77.7   52.3   aacC2   S68058   70-       IIa   TTC GAA TTG CCT CCG TTA TTG                           aacC(2)200       (aacC2)   CCT TCC GCG TAT GCA TCG CGA           TAT CTC C (SEQ ID NO:9)               aac(3)-   TCG ATC AGT CCA AGT GGC CCA   70   380-311   82.7   62.9   aac(3)-IV   X01385   70-       IV   TCT TCG AGG GGC CGG ACG CTA                           aac3(IV)380           CGG AAG GAG CTG TGG ACC AGC           AGC ACA C (SEQ ID NO:10)               aph(3′)-   GGC GCA TCG GGC TTC CCA TAC   70   1310-   79.1   54.3   aphA1, aphA7,   V00359   70       Ia   AAT CGA TAG ATT GTC CCT GAT       1241           strA,       aphA(1)1310       (aphA1)   TGC CCG ACA TTA TCG CGA GCC                   Tn903           CAT T (SEQ ID NO:11)               aph(3′)-   AGT CAT AGC CGA ATA GCC TCT   70   220-151   78.9   52.9   Tn5, aphA2,   V00618   70-       Ila   CCA CCC AAG CGG CCG GAG AAC                   aph(3′)       aphA(2)220       (aphA2)   CTG CGT GCA ATC CAT CTT GTT           CAA TCA T (SEQ ID NO:12)               tet(A)   GAT GCC GAC AGC GTC GAG CGC   70   1390-   79.5   57.1   tetA   X00006   70-tetA1390           GAC AGT GCT CAG AAT TAC GAT       1321           CAG GGG TAT GTT GGG TTT CAC           GTC TGG C (SEQ ID NO:13)               tet(B)   CAA AGT GGT TAG CGA TAT CTT   70   190-121   71.8   40   tetB,Tn10   V00611   70-tetB190           CCG AAG CAA TAA ATT CAC GTA           ATA ACG TTG GCA AGA CTG GCA           TGA TAA G (SEQ ID NO:14)               tet(C)   GAC TGG CGA TGC TGT CGG AAT   70   130-61   80.8   58.6   pBR322, RP1,   J01749   70-tetC130           GGA CGA TAT CCC GCA AGA GGC                   tetC...           CCG GCA GTA CCG GCA TAA CCA           AGC CTA T (SEQ ID NO:15)               tet(D)   CAA ACG CGG CAC CCG CCA GGG   70   1770-   83.5   64.3   tetA   X65876   70-tetD1770           ATA ACA GCA GCA CCG GTC TGC       1701           GCC CCA GCT TAT CTG ACC ATC           TGC CCA G (SEQ ID NO:16)               tet(E)   GTT GAG GCT GCA ACA GCT CCA   70   370-301   78   51.4   tetE   L06940   70-tetE370           GTC GCA CCG GTA ATA CCA GCA           ATT AAG CGT CCC AAA TAC AAC           ACC CAC A (SEQ ID NO:17)               tet(Y)   TTA ATA AAG CCG GAA CCA CCG   70   1770-   76.5   47.1.   tetY   AF070999   70-tetY1770           GCA TGA TTA ATC CCA AAC CAA       1701           TCG CAT CAA GCG CGA CAA           CAA TGA GTG C (SEQ ID NO:18)               catI   TTT ACG GTC TTT AAA AAG GCC   70   550-481   73.1   41.1   cam, Tn9, R100,   M62822   70-cat550           GTA ATA TCC AGC TGA ACG GTC                   cat,...           TGG TTA TAG GTA CAT TGA GCA           ACT GAC T (SEQ ID NO:19)               catII   AGC GGT AAT ATC GAG TTT GGT   70   300-231   75.6   45.7   catII   X53796   70-cat(2)           GGT CAG GCT GAA TCC GCA TTT                       300           AAT CTG CTG ACG ATA AAG GGC           AAA GTG T (SEQ ID NO:20)               catIII   TTT GCT TGT TAA GCT AAA ACC   70   370-301   74.4   41.4   catIII   X07848   70-cat(3)           ACA TGG TAA ACG ATG CCG ATA                       370           AAA CTC AAA ATG CTC ACG GCG           AAC CCA A (SEQ ID NO:21)               floR   GAC AAA GGC CGG TGC AGT TGA   70   384-315   82.3   60   floR, pp-flo   AF252855   70-floR384           AGA CCA AGC TGC TCC CAG AGA           CGC AAT GAC GAA AGC CGT TGC           GCC CGC A (SEQ ID NO:22)               dhfr1   GGT TAA AGC ATC TTT AAT TGA   70   490-421   69.2   32.9   dhfrl, Tn9   X00926   70-           TGG AAA GAT CAA TAC GTT CTC                           dhfr(1)490           ATT GTC AGA TGT AAA ACT TGA           ACG TGT T (SEQ ID NO:23)               dhfrV   GTA CAT GGC CTC TTC GAT CGA   70   1560-   76.6   51.4   dhfrV,dhfrlb,   X12868   70-           CGG GAA TAC TAT TAC GTT GTC       1491           dhfrXVI       dhfr(5)1560           ATT ATG GGC CGT CCA GGC TGA           GCG AGT A (SEQ ID NO:24)               dhfrVII   GAA CAC CCA TAG AGT CAA ATG   70   753-684   64.2   72.4   dhfrVII, dhfrXVII   X58425   70-           TTT TCC TTC CAA CAA GGA GCC                           dhfr(7)753           ACT GAT TAT ATG TGA GCG CTT           TAA AGA G (SEQ ID NO:25)               dhfrIX   AGC TTT GAA GTG TTT TAA ATC   70   830-761   72.5   40   dhfrlX   X57730   70-           TTG TGG TTC ATG CCA CGG AAT                           dhfr(9)830           CTG ATT TTC AAA TCC GAT ACC           TCC TGT C (SEQ ID NO:26)               dhfrXIII   TGG CGC GAG AGC ACC ACT GTG   70   929-860   82.1   58.6   dhfrXIII   Z50802   70-           TGG CGG TTT GGT AAG GGC TTG                           dhfr(1 3)929           CCT ATG GAC TCA AAT GTC TTG           CGG CCC A (SEQ ID NO:27)               dhfrXV   CTT CAG ATG ATT TAG CGC TTC   70   620-551   71.2   38.6   dhfrXV   Z83311   70-           ATC GAT AGA TGG AAA TAC CAA                           dhfr(15)620           TAC ATT CTC ATC ACT GGA AGT           GAA GCT T (SEQ ID NO:28)               suII   AGC GCC GGC GGG GTC TAG CCG   70   960-891   82.5   62.9   Tn2l, Integron   X12869   70-sul           GCG GCT CTC ATC GAA GAA GGA                   class       (1)960           GTC CTC GGT GAG ATT CAG AAT                   1, sulI           GCC GAA C (SEQ ID NO:29)               suIII   TAC GCG CCT GCG CAA TGG CTG   70   420-351   82.8   61.4   RSF1010, sulII   M36657   70-sul(2)420           CGT CTG GCG CCA GAT ACC GGC           CTC CAT CGG AGA AAC TGT CCG           AGG TTA T (SEQ ID NO:30)               integron   TTG GAT GCC CGA GGC ATA GAC   70   1200-   78.3   51.4   integrase, Int1   M33633   70       classe 1   TGT ACC CCA AAA AAC AGT CAT       1131                   int(1)1200       3″-CS   AAC AAG CCA TGA AAA CCG CCA           CTG CGC C (SEQ ID NO:31)               Gram       postive       vat   TTT ACC GAT AAA AGG GAA TCG   70   2822-   68.3   31.4   AF117258   70-vat2822           GAA TCT TCA ATT TAT AAA ACC       2753           TAC TAT AAC GAA CGA AAA CAT           TTT GGT G (SEQ ID NO:32)               vatC   GAA CAT GTT TAT TAC CTT CTA   70   1376-   71   37.1   AF015628   70-           TAG GGT ATA TTT CTT CTG GAT       1307               vatC1376           TGG GGC CTT GCT GAT TTT GCC           ATT TCA T (SEQ ID NO:33)               vatD   TTG ATC TAA TTT TGG CAT ATG   70   3022-   69.2   32.9   AF368302   70-           TTT CTC CCA TCC ATT ACC AAA       2953               vatD3022           TAA ATT AAA TGG ATA TGT TGA           GCC ATC C (SEQ ID NO:34)               vatE   CGT TCT TGA TAA AGT CTA GCT   70   70-1   71.7   41.4   AY043213   70-vatE70           CTA TGA GGA TGA GGT TAG GAT           AGA CTG CAT TTG CGT CAG GTA           TAG TCA T (SEQ ID NO:35)               vga   GAG CTT CAA TTG AGG AAT AAG   70   1133-   69.1   31.4   M90056   70-vga1133           TTC ACA ATG TGA AAA TTG TTT       1064           TAC AAT ACC TTC TTC AGG CAC           AAT TTT T (SEQ ID NO:36)               vgb   CCA TAT GGT ATA ACC GTT TCA   70   3720-   68.8   32.9   AF117258   70-Vgb3720           GAT AAG GGG AAA GTT TGG ATT       3651           ACA CAA CAT AAA GCA AAT ATG           ATA AGT T (SEQ ID NO:37)               vgbB   CTG ATG AAG TTA TAC CGT ATG   70   468-399   68.8   34.3   AF015628   70-vgbB468           GAC CTG AAT CGG GAA TAG ACA           AGT TAA ACT CCT CTA AAT AAA           AAT TCA T (SEQ ID NO:38)                  
 
         [0081]     The present invention will be more readily understood by referring to the following examples which are given to illustrate the invention rather than to limit its scope.  
       EXAMPLE I  
       [0082]     Taxonomic Identification of Microorganisms Present in a Commercial Consortium  
         [0083]     The following experiment was conducted to establish the concept of the invention and obtain preliminary results. A DNA microarray slide (Corning Ultragaps, Corning, N.Y.) was printed with DNA sequences using conventional technique in the art for attaching on the slide a number of sequences of genes as detailed in Table 2 below.  
                                 TABLE 2                       Interpretation key (probes for Biozyme 5000 in grey)                                + cont GFP     A. oryzae  18S     P. denitrificans  nos Z   + cont GFP       − cont GFP     A. oryzae  pepO     P. fluorescens  16S   − cont GFP                 A. globiformis  23S                                   R. eutropha  16S     A. Hydrophila  aly                 A. oxydans  recA                                   S. cerevisiae  18S     A. salmonicida  bhem1                                               B. megaterium  cpn 60     S. typhi  dlt     A. globiformis  16S                 C. jejuni  gtpase     B. megaterium  merR2     S. scabies  16S     A. globiformis  est                 C. albicans  MNT1                                   S. elongatus  16S     A. oxydans  16S                 E. coli  stx2A     B. cepacia  pvdA                                   A. niger  calnexin               + cont  A. thaliana       N. winogradskyi  16S     P. denitrificans  nir S     C. jejuni  gtpase                                               N. europa  amo A                                   C. albicans  MNT1                 S. elongatus  16S   Nitrosomonas nir K                                   C. parvum  lax               Buffer     P. denitrificans  nir S     P. denitrificans  nos Z     E. faecalis  16S               Buffer                                   R. eutropha  gyrB     E. coli  gus A               Buffer                                   S. scabies  16S     E. coli  sltlle               Buffer     P. aeruginasa  16S     S. elongatus  16S     K. pneumoniae  cpn 60       Buffer     P. aeruginosa  toxA   + cont  A. thaliana       N. hambergensis  nor B                  
 
         [0084]     Table 3 lists the oligonucleotides probes immobilized on the microarray prototype used to analyze a commercial consortium; the layout of the microarray found in Table 2.  
                                                     TABLE 2                           Sequences of the probes for Biozyme 5000                        Gen Bank           GC               Probe   Organism   Gene   Accession no.   Start   Sequence   %   Tm               A.     A. oryzae     pepO   ASNPEPA   721-770   TTTCCAGAAGGCTTGTAGACGTCGTGGCCGTCTGCTCGGACTTGG   64   84                               GGAG (SEQ ID NO:39)               B.     A. globiformis     23S   ARG23RRNAD   11-60   CACCCACAAGGGGTGTCAGGCAGGTCTCGGGCGGTTAGTATCCCC   62   83                           TGTTC (SEQ ID NO:40)               C.     A. oxydans     recA   AF214789   1-50   TCCCAAAGCAACATCCAGGGCAATGGATCCGGTGGGGATGACCTC   58   82                           GATCG (SEQ ID NO:41)               D.     B. subtilis     16S-23S   BSUB0005   144980-   GMCACGTTTCGMGGAATGATCCTTCAAAACTAAACAAGACAGGGA   42   74                       145029   ACG (SEQ ID NO:42)               E.     A. salmonicida     bhem1   AS17BHEM1   1490-   AGTCTCGTCACAGGTCACGGCGCTCAGGCCATGCTCGGCGCCGGC   70   80                       1539   GCTCA (SEQ ID NO:43)               F.     s. typhi     dlt   STDLT   661-710   GAAGGCGGCATTGTTGATATGGTAACGGCCACGGACATACACGGA   56   74                           AGGCG (SEQ ID NO:44)               G.     P. denitrificans     nosZ   AF016059   1046-   TTCTCCGGGTGCAGCGGGCCGGTGGGCAGGAAGCGGTCCTTGGAG   66   86                       1095   AACTT (SEQ ID NO:45)               H.     P. fluorescens     16S   PSEIAM12   48-97   CCGTCCGCCTCTCTCAAGAGAAGCAAGCTTCTCTCTACCGCTCGA   58   81                           CTTGC (SEQ ID NO:46)               I.   R. eutropha   16S   AFARGSSA   146-195   CGCTTTCACCCTCAGGTCGTATGCGGTATTAGCTAATCTTTCGAC   46   70                           TAGTT (SEQ ID NO:47)               J.     A. globiformis     16S   AGRDNA16   66-115   GGGCAGGTTACTCACGTGTTACTCACCCGTTCGCCACTAATCCCC   60   82                           GGTGC (SEQ ID NO:48)               K.     S. scabies     16S   AB026210   67-116   CGTGTTACTCACCCGTTCGCCACTAATCCCCACCGAAGTGGTTCA   54   74                           TCGTT (SEQ ID NO:49)               L.     A. globiformis     esterase   E04386   51-100   AGGCCGCGAGCTGGGCTGAATATTCCCGGTCTTCGCTCAGGAAAC   62   84                           GGCCA (SEQ ID NO:50)               M.     N. winogradskyi     16S   NITRGDW   46-95   ACGCGTTACTCACCCGTCTGCCACTGACGTATTGCTACGCCCGTT   58   82                           CGACT (SEQ ID NO:51)               N.     P. polymyxa     16S   AJ223989   66-115   TTACTCACCCGTCCGCCGCTAGGCTTATATAGAAGCAAGCTTCTA   48   71                           CGATA (SEQ ID NO:52)               O.     P. polymyxa     16S   AJ223989   66-115   TTACTCACCCGTCCGCCGCTAGGCTTATATAGAAGCAAGCTTCTA   48   71                           CGATA (SEQ ID NO:53)               P.     S. elongatus     16S   AF410931   206-255   TGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCCCACTGCTG   60   84                           CCTCC (SEQ ID NO:54)               Q.     S. elongatus     16S   AF410931   206-255   TGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCCCACTGCTG   60   84                           CCTCC (SEQ ID NO:55)               R.     Eubacterial     16S   ECRRNBZ   325-374   TTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCG   56   81                           TGTCT (SEQ ID NO:56)               S.     P. aeruginosa     16S   AB037563   52-101   TCACCCGTCCGCCGCTGAATCCAGGAGCAAGCTCCCTTCATCCGC   64   54                           TCGAC (SEQ ID NO:57)               T.     P. aeruginosa     toxA   AF227421   121-170   GAAGGTGCCGTGGTAGCCGACGAACACATAGCCGCGCTCCTCCAG   64   84                           TTGGC (SEQ ID NO:58)               U.     R.eutropha     gyrB   A6014982   51-100   CTGTGGATGGTGACCTGGATCTCGGTGCAGTAGCCGGCCAGCGCT   64   84                           TCGTC (SEQ ID NO:59)               V.     P. denitrificans     nosZ   AF016059   860-909   TCTTCCAGGTTCCATTTGACCAGCTGGCTGTCGATGAACAGCGTG   52   73                           GTGTA (SEQ ID NO:60)               W.     N. hamburgensis     norB   NHNORB   821-870   CCAGTTGAAGTAGGTCTTCTTGTACGGGCAGCCGGAGACGCACAT   60   82                           GCGCC (SEQ ID NO:61)               X.     C. albicans     MNT   CAMNT1PRT   121-170   CCAGCAGCAACATTACCGGTCTGTTTTTCATGAGCGGCGGGTGAT   50   80                           TGTGT (SEQ ID NO:62)               Y.   Negative control   GFP.   AEVGFP   595-644   GGCCTAGAGGGTCCTGTTCGCAGGTGATAAAAGGATGAGGGAAAT   52   73           1               GTCGT (SEQ ID NO:63)               Z.   Negative control   GFP.   AEVGFP   371-420   ACACACCTAACTAGTAAACGTTTAATTTCAATCTTTTGCACATCA   30   64           2               TAGTT (SEQ ID NO:64)                  
 
         [0085]     The DNA microarray slide was then hybridized overnight at 42° C. for 16 hours with 500 ng of Biozyme 5000 (Mirus B (6 Sep. 2002)) DNA. The DNA had previously been labeled with Cy3 16% in DIG hyb buffer: The hybridization volume was 6 μl on a cover slip of dimension 11 mm×11 mm. After hybridization, the cover slip is removed in 1×SC at room temperature followed by three washes. The first wash is made in 1xSSC, 0.2% SDS at 37° C. for 10 minutes. The second wash is made in 0.1xSSC, 0.2% SDS at room temperature for 5 minutes. Finally the third wash is made in 0.1xSSC, at room temperature for 5 minutes.  
         [0086]     As a result, the interpretation key for the triplicate probes (see Table 2) identifies which spots represent which genes. Probes for any of the three bacterial species claimed to be present in the Biozyme 5000 consortia ( B. subtilis, B. licheniformis , and  P. polymyxa )are highlighted in grey. The strong signals were obtained from the expected microorganism  Bacillus subtilis  (see  FIG. 2 ).  
       EXAMPLE II  
     Discriminating Power of cpn60 Probes Between Two Bacilleaceae Species  
       [0087]     A microarray plate as in example I above with the same array layout and probe sequences is being used herein to illustration the superior specificity of cpn60 probes compared to 16S probes. The left panel ( FIG. 3A ) shows fluorescent labelled DNA from  B. megaterium  applied to array. The right panel ( FIG. 3B ) shows fluorescently labelled DNA from B licheniformis applied to array. The results obtained are illustrated in  FIGS. 3A and 3B . As can be seen in  FIGS. 3A and 3B , the cpn60 probe specific for  B. licheniformis  gives a signal when hybridized with  B. licheniformis  genomic DNA, but not at all with  B. megaterium  genomic DNA and vice versa (upper panels). This is not the case with the 16S probes (lower panels) that seem to light up much more easily and cross react with other 16S probes for different species. This results demonstrates the extra resolving power of cpn60 probes  
       EXAMPLE III  
     Microarray Using 16S and cpn60 Amplicons  
       [0088]     A microarray plate was printed with the following sequences found in Table 3 using the key found in  FIG. 4A .  
                                     TABLE 3                           SEQUENCES USED FOR AMPLICON ARRAY                    GenBank               Organism   Gene   Accession no.   Sequence                 subtilis     16S   ATCC 9799   TGTTAGGGAAGAACAAGTGCCGTTCAAATAGGGCGGCACCTTGACGGTACCTAACCAGAAAGCCACGGCT   +TL,64                   AACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAACGGC                   TCGCAGGCGCTTTCTTAAGTCTGATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGCGG                   AACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACA                   CCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGAGCGAAAOCGTGGGGAGCGAACAGGAT                   TAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGGGGGTTTCCGCCCCTTAGTGCT                   GCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGG                   GGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCACGTCTTGACAT                   CCTCTGACAATCCTAGAGATAGGACGTCCCCTTCGGGGGCAGAGTGACAGGTGGTGCATGGTTGTCGTCA                   GCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCA                   GTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCC                   CCTTATGACCTGGGCTACACACGTGCTACAATGGGCAGAACAAAGGGCAGCGAAACCGCGAGGTTAAGCC                   AATCCCACAAATCTGTTCTCAGTTCGGATCGCAGTCTGCAACTCGACTGCGTGAAGCTGGAATCGCTAGT                   AATCGCGGATCAGATGCCGGGTGATACGTTCCCGGGCCTTGTACACCGCCCGTCACACCACGAGAGTTTG                   TAACACCCGAAGTCGGTGAGGTAACCTTTTTGGAGCCAGCCGCCGAAGGTGGGACAGATGATTGGGTTC                   (SEQ ID NO: 65)                 Bacillus     16S   DSM 13   AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGGACCGACG         licheniformis             GGAGCTTGCTCCCTTAGGTCAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTGTAAGACTGGG                   ATAACTCCGGGAAACCGGGGCTAATACCGGATGCTTGATTGAACCGCATGGTTCAATCATAAAAGGTGG                   CTTTTAGCTACCACTTACAGATGGACCCGCCGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGG                   CGACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTAC                   GGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGA                   AGGTTTTCGGATCGTAAAACTCTGTTGTTAGGGAAGAACAAGTACCGTTCGAATAGGGCGGTACCTTGA                   CGGTACCTAACCAGAAAGCCACGCCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGT                   TGTCCCGAATTATTGGGCGTAAAGCGCGCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCCCGGCTC                   AACCGGGGAGGGTCATTGGAAACTCGGGAACTTGAGTGCAGAAGAGGACAGTGGAATTCCACGTGTAGC                   CGTGAAATGCGTAGAGATGTCGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTG                   AGGCGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCT                   AAGTGTTAGAGGGTTTCCGCCCTTTAGTGCTGCAGCAAACGCATTAAGCACTCCGCCTGGGGAGTACGG                   TCGCAAGACTGAAACTCAAAGGAATTGACGCGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGA                   TAGAGATAGGGCTTCCCCTTCGGGCGCAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTG                   AGATGTTGCGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAGTTGGGCACTC                   TAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACC                   TGGGCTACACACGTGCTACAATGGGCAGAACAAAGGGCAGCGAAGCCGCGAGGCTAAGCCAATCCCACA                   AATCTGTTCTCAGTTCGGATCGCAGTCTGCAACTCGACTGCGTGAAGCTGGAATCGCTAGTAATCGCGG                   ATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTCTACACACCGCCCGTCACACCACGAGAGTTTGTA                   ACACCCGAAGTCGGTGAGGTAACCTTTTGGAGCCAGCCGCCGAAGGTGGGACAGATGATTGGGGTGAAG                   TCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCT (SEQ ID NO:66)                 Bacillus     16S   NCDO   GAGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGGACAGAA         pumilus         1766   GGGAGCTTGCTCCCGGATGTTAGCGGCGGACGGGTGAGTAACNCGTGGGTAACCTNCCTGTNAGACTGG                   GATAACTCCGGGAAACCGGAGCTAATACCGGATAGTTCCTTGAACCGCATNGTACAAGGATGAAAGACC                   GTNTCGGCTATCACTTACAGATNGACCCGCGGCCCATTAGCTAGTTGGTGGGGTAATGGCTCACCAAGG                   CGACGATGCGTAGCCGACCTGAGAGGGTNATCGGCCACACTGGGACTGAGACACGGCCNNGACTCCTAC                   GGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGA                   AGGTTTTCGGATCGTNAAGCTCTGTTGTTAGGGAAGAACAAGTGCGAGAGTAACTNCTCGCACCTTGAC                   GGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTNATACGTAGGTGGCAAGCGTT                   GTCCGGAATTATTGGGCGTNAAGGGCTCGCAGGCGGTTTCTTAAGTCTNATGTGAAAGCCCNCNGCTCA                   ACCGGGGAGGGTCATTGGAAACTGGGNAACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCG                   GTNAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTNGTCTGTAACTNACGCTGA                   GGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTN                   AGTGTTAGGGGGTTTCCGCCCCTTAGTGCTNCANCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGT                   CGCAAGACTNAAACTCAAAGGAATTGACGGGGGCCNGCACAAGCGGTGGAGCATGTNGTTTAATTCGAA                   GNAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACAACCCTAGAGATAGGGNTNTCTTCGGGGA                   CAGAGTGACAGGTGGNGCATNGTNGTCGTCAGCTCGTGTCGTGAGATGTTGGOTTAAGTCCCGCAACGA                   GCGCAACCCTTGATCTTAGTTGCCAGCATTTAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGG                   AGGAAGGTNGGGATGACGTCAAATCATCATGCCCCTTATGACCTNGGCTACACACGTGCTACAATGGAC                   AGACNAAGGGCTGCGAGACCGCAAGGTTTAGCCAATCCCATAAATCTGTTCTCAGTTCGGATCGCAGTC                   TGCNACTNGACTGCGTGAAGCTGGAATCGCTAGTAATCGCGGATCGCATOCCGCGGTGAATACGTTC                   CCGGGCCTNGTACACACCGCCCGTCACACCACGAGAGTTTGNAACACCC (SEQ ID NO:67)                 B. amylolique-     16S   ATCC   GAGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGGACAGAT         faciens         23350   GGGAGCTTGCTCCCTGATGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTGTAAGACTGG                   GATAACTCCGGGAAACCGGGGCTAATACCGGATGCTTGTTTGAACCGCATGGTTCAACATAAAGGTGGC                   TTCGGCTACCACTTACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCG                   ACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGG                   GAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAG                   GTTTTCGGATCGTAAAGCTNTGTTGTTAGGGAAGAACAAGTGCCGTTCAAATAGGGCGGNACCTNGACG                   GTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTNATACGTAGGTGGCNAGCGTTG                   TCCGGAATTNTTGGGCGTNAAGGGCTCGCAGGCGGTTTCTTNAGTCTGATGTGAAAGCCCCCGGCTCAA                   CCGGGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGG                   TGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTTGTAACTGACGCGAGGA                   GCGAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTG                   TTAGGGGGTTTCCGCCCCTTAGTGCTGCAGTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAA                   GACTNAAACTCAAAGGAATTGACGGGGCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGC                   GAAGAACCTTACCAGGTCTTGACATCCTCTGACAATCCTAGAGATAGGACGTCTTCGGGGGCAGAGTGA                   ACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAAC                   CCTTGATCTTAGTTGCCAGCATTCAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGG                   TGGGGATGACGTCAAATCATCATGCCCCTTATGACCTNGGCTACACACGTGCTACNATGGGCAGAACNA                   AGGGCAGCGAAACCGCGAGGTCAAGCCAATCCCACAAATCTATTCTCAGTTCGGATCGCAGTCTGCAAC                   TCGACTGCGTGAAGCTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCC                   TTGTACACACCGCCCGTCACACCACGAGAGTTTGTAACACCC (SEQ ID NO:68)                 Bacillus     16S   IAM   GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAATGGATTAAGAGCTTGCTCTTATGAAG         cereus         12605   TTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCCATAAGACTGGGATAACTCCGGGAAACCGG                   GGCTAATACCGGATAACATTTTGAACCGCATGGTTCGAAATTGAAAGGCGGCTTCGGCTGTCACTTATG                   GATGGACCCGCGTCGCATTAGCTAGTTGGTGAGGTAACGGGCTCACCAAGGCAACGATGCGTAGCCGAC                   CTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGA                   ATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGCTTTCGGGTCGTAAA                   ACTCTGTTGTTAGGGAAGAACAAGTGCTAGTTGAATAAGCTGGCACCTTGACGGTACCTAACCAGAAAG                   CACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCG                   TAAAGCGCGCGCAGGTGGTTTCTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGG                   AAACTGGGAGACTTGAGTGCAGAAGAGGAAGTGGATTCCATGTGTAGCGGTGAAATGCGTAGAGATATG                   GAGGAACACCAGTGGCGAAGGCGACTTTCTGGTCTGTAACTGACACTGAGGCGCGAAAGCGTGGGGAGC                   AAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGCC                   CTTTAGTGCTGAAGTTAACGCATTAAGCACTCCGCCTGGGGAGTACGGCCGCAAGGCTGAAACTCAAAG                   GAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACC                   AGGTCTTGACATCCTCTGAAAACCCTAGAGATAGGGCTTCTCCTTCGGGAGCAGAGTGACAGGTGGTGC                   ATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTA                   GTTGCCATCATTAAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAGGTGGGGATGACGT                   CAAATCATCAGTGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACGGTACAAAGAGCTGCAAG                   ACCGCGAGGTGGAGCTAATCTCATAAAACCGTTCTCAGTTCGGATTGTAGCTGCAACTCGCCTACATGA                   AGCTGGATCGCTAGTAATCGCGGATCAGATGCCGCGGTGATACGTTCCCGGCCTTGTACACACCGCCCG                   TCACACCACGAGAGTTTGTAACACCCGAGTCGGTGGGGTAACCTTTTTGGAGCCAGCCGCCTAAGGTGG                   GACAGATGATTGGGGTGAAGTCGTAACAA (SEQ ID NO:69)                 Bacillus     16S   NUB3621   GCGGCGTGCCTAATACATGCAGTCGAGCGGACTCGCGGCGAGCTTGCTTTGCCTTGGTCAGCGGCGGAC         stearothermo-             GGGTGAGTAACACGTGGGTAACCTGCCCGCAAGACCGGGATAACTCCGGGAACCGGGGCTAATACCGGA         philus             TAACACCGAGACCGCATGGTCTTCGGTTGAAAGGCGGCTTCGGCTGCCACTTACTGATGGGCCCGCGGC                   GCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCGACGATGCGTAGCCGGCCTGAGAGGGTGACCG                   CACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGAATCTTCCCAATGGACGAA                   AGTCTGACGGAGCGACGCCGCGTGAGCGAAGAAGGCCTTCGGGACGTAAAGCTCTGTTGTTAGGGAAGA                   AGAAGTGCCGTTCGAACAGGGCGGTCCGGTGAACGTACCTACCGAGAAAGCCCCGGCTAACTACGTGCC                   AGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGG                   TCCCTTAAGTCTGATGTGAAAGCCCACGGCTTAACCGTGGAGGGTCATTGGAAACTGGGGGACTTGAGT                   GCAGGAGAGGAGACGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCG                   AAGGCGGCTCTCTGGTCCGTCTCTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACC                   CTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGGTATTCCCTTTAGTGCTGTATCTAA                   CGCGTTAAGCACTCCGCCTGGGGAGTACGGCCGCAAGGCTGAAACTCAAAGGAATTGACGGGGGCCCGC                   ACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCCCTG                   ACAACCCTGGAGACAGGGCGTTCCCCCCTTGCGGGGACAGGGTGACAGGTGGTGCATGGTTGTCGTCAG                   CTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAAGCGCAACCCTCGCCCCTAGTTGCCAGCATTCATTT                   GGGCACTCTAGGGGGACTGCCGGCTAAAACTCAGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCC                   TTATGACCTGGGCTACACACGTGCTACAATGGGCGGTACAAAGGGCTGCGAACCCGCGAGGGGGAGCGA                   ATCCCAAAAAGCCGCTCTCAGTTCGGATTGCAGGCTGCAACTCGCCTGCATGAAGCCGGAATCGCTAGT                   AATCGCGATCAGCATGCCCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAG                   CTTGCAACACCC (SEQ ID NO:70)                 Bacillus     16S   IAM   GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAACTGATTAGAAGCTTGCTTCTATGACG         megaterium         13418   TTAGCGGCGGACGGGTGAGTAACACGTGGGCAACCTGCCTGTAAGACTGGGATAACTTCGGGAAACCGA                   GGCTAATACCGGATAGGATCTTCTCCTTCATGGGAGATGATTGAAAGATGGTTTCGGCTATCACTTACA                   GATGGCCCGGGTGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCACGATGCGTAGCCGACCTGA                   GAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCT                   TCCGCAATGGACGAAACTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGCTTTCGGGTCGTAAAACTC                   TGTTGTTAGGGAAGAACAAGTACAAGAGTAACTGCTTGTACCTTGACGGTACCTAACCAGAAAGCCACG                   GCTAACTACGTGCCAGCAGCCGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGGAACTT                   GAGTGCAGAAGAGAAAAGCGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAG                   TGGCGAAGGCGGCTTTTTGGTCTGTAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAG                   ATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGCCCTTTAGTGCTGC                   AGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGG                   GCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACAT                   CCTCTGACAACTCTAGAGATAGAGCGTTCCCCTTCGGGGGACAGAGTGACAGGTGGTGCATGGTTGTCG                   TCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCA                   TTTAGTTGGTGCACTTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATC                   ATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGATGGTACAAGGGCTGCAAGACCGCGAGGTC                   AAGCCAATCCCATAAAACCATTCTCAGTTCGGATTGTAGGCTGCAACTCGCCTACATGAAGCTGGAATC                   GCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACAC                   CACGAGAGTTTGTAACACCCGAAGTCGGTGGAGTAACCGTAAGGACGTAGCCGCCTAAGGTGOGACAGA                   TGATTGGGGTGAAGTCGTAACAA (SEQ ID NO:71)                 Bacillus     16S   IAM   GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGTGCGGACCTTTTAAAAGCTTGCTTTTAAAAGG         coagulans         12463   TTAGCGGCGGACGGGTGAGTAACACGTGGGCAACCTGCCTGTAAGACNGGGATAACGCCGGGAAACCG                   GGGCTAATACCNGATAGTTTTTTCCTCCGCATGGAGGAAAAAGGAAAGGCGGCTTCGGCTGCCACTTAC                   AGATGGGCCCGCGGCGCATTAGCTAGTTGGCGGGGTAACGGCCCACCAAGGCAACGATGCGTAGCCGAC                   CTGAGAGGGTGATCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTAGGGA                   ATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGAAGAAGGCCTTCGGGTCGTAAA                   ACTCTGTTGCCGGGGAAGAACAAGTGCCGTTCGAACAGGGCGGCGCCTTGACGGTACCCGGCCAGAAAG                   CCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGC                   GTAAAGCGCGCGCAGGCGGCTTCTTAAGTCTGATGTGAAATCTTGCGGCTCAACCAAGCGGTCATTGGA                   AACTGGGAGGCTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGT                   GGAGGAACACCAGTGGCGAAGGCGGCTCTCTGGTCTGTAACTGACGCTGAGGCGCGAAAGCGTGGGGAG                   CAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGC                   CCTTTAGTGCTGCACTAACGCATTAAGCACTCCGCCTGGGGAGTACGGCCGCAAGGCTGAAACTCAAAG                   GAATTGACGGGGGCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCA                   GGTCTTGACATCCTCTGACCTCCCTGGAGACAGGGCCTTCTTCGGGGGACAGAGTGACAGGTGGTGCAT                   GGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGACCTTAGT                   TGCCAGCATTGAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTC                   AAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGATGGTACAAAGGGCTGCGAGAC                   CGCGAGGTTAAGCCAATCCCAGAAAACCATTCCCAGTTCGGATTGCAGGCTGCAACCCGCCTGCATGAA                   GCCGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGC                   CCGTCACACCACGAGAGTTTGTAACACCCGAAGTCGGTGAGGTAACCTTTANGGAGCCAGCCGCCGAAG                   GTGGGACAGATGATTGGGGTGAAGTCGTAACAA (SEQ ID NO:72)                 Alicycloba-     16S   DSM 446   CCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGGACTTTTCGGAGGTCAGC         cillus             GGCGGACGGGTGAGGAACACGTGGGTAATCTGCCTTTCAGACCGGAATAACGCCCGGAAACGGGCGCTA         acidocaldarius             ATGCCGGATACGCCCGCGAGGAGGCATCTTCTTGCGGGGAAAGGCCCGATTGGGCCGCTGAGAGAGGAG                   CCCGCGGCGCATTAGCTGGTTGGCGGGGTAACGGCCCACCAAGGCGACGATGCGTAGCCGACCTGAGAG                   GGTGACCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCC                   GCAATGGGCGCAAGCCTGACGGAAGCAACGCCGCGTGAGCGAAGAAGGCCTTCGGGTTGTAAAGCTCTG                   TTGCTCGGGGAGAGCGGCATGGGGAGTGGAAAGCCCCATGCGAGACG                   GTACCGAGTGAGGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAAAACGTAGGGGGCGAGCGTTG                   TCCGGAATCACTGGGCGTAAAGGGTGCGTAGGCGGTCGAGCAAGTCTGGAGTGAAAGTCCATGGCTCAA                   CCATGGGATGGCTCTGGAAACTGCTTGACTTCAGTGCTGGAGAGGCAAGGGGAATTCCACGTGTAGCGG                   TGAAATGCGTAGAGATGTGGAGGAATACCTGTGGCGAAGGCGCCTTGCTGGACAGTGACTGACGCTGAG                   GCACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAG                   GTGTTGGGGGGACACACCCCAGTGCCGAAGGAAACCCAATAAGCACTCCGCCTGGGGAGTACGGTCGCA                   AGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCAGTGGAGCATGTGGTTTAATTCGAAGCAA                   CGCGAAGAACCTTACCAGGGCTTGACATCCCTCTGACCGGTGCAGAGATGCACCTTCCCTTCGGGGCAG                   AGGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTCAGTCCCGCAACGAGC                   GCAACCCTTGACCTGTGTTACCAGCGCGTTGAGGCGGGGACTCACAGGTGACTGCCGGCGTAAGTCGGA                   GGAAGGCGGGGATGACGTCAAATCATCATGCCCCTGATGTCCTGGGCTACACACGTGCTACAATGGGCG                   GTACAAAGGGAGGCGAAGCCGCGAGGCGGAGCGAAACCCAAAAAGCCGCTCGTAGTTCGGATTGCAGGC                   TGCAACTCGCCTGCATGAAGCCGGAATTGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCC                   CGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTCGOCAACACCCGAAGTCGGTGAGGTAACCCCG                   GGAAGGCGGGGATGACGTCAAATCATCATGCCCCTGATGTCCTGGGTCGTAACAAGGTAGCCGTACCGG                   AAGGTGCGGCTG (SEQ ID NO:73)                 Bacillus     16S   NCIMB   AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAATGGATG         lentus         8773   GGAGCTTGCTCCCAGAAGTTAGCGGCGGACGGGTGAGTAACACGTGGGCAACCTACCTGTAAGACTGGG                   ATAACTTCGGGAAACCGGAGCTAATACCGGATAACTTCTTTCTTCTCCTGGAGAAAGGTTGAAAGACGG                   CTTCGGCTGTCACTTACAGATGGGCCCGCGGCGCATTAGCTACTTGGTGAGGTAACGGCTCACCAAGGC                   AACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACG                   GGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAA                   GGTTTTCGGATCGTAAAACTCTGTTATCAGGGAAGAACAAGTATCGGAGTAACTGCCGGTACCTTGACG                   GTACCTGACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG                   TCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCACGGCTCAA                   CCGTGGAAGGTCATTGGAAACTGGGAGACTTGAGTGCAGAAGAGAAGAGCGGAATTCCACGTGTAGCGG                   TGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGGCTCTTTGGTCTGTAACTGACGCTGAG                   GCGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAA                   GTGTTAGAGGGTTTCCGCCCTTTAGTGCTGCAGTTAACGCATTAAGCACTCCGCCTGGGGAGTACGGCC                   GCAAGGCTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAG                   CAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACCACCCTAGAGATAGGGACTTCCCCTTCGGG                   GGACAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAA                   CGAGCGCAACCCTTAACCTTAGTTGCCAGCATTCAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAAC                   CGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATG                   GATGGTACAAAGGGTTGCAAGACCGCGAGGTTTAGCTAATCCCATAAAACCATTCTCAGTTCGGATTGC                   AGGCTGCAACTCGCCTGCATGAAGCCGGAATCGCTAGTAATCGTGGATCAGCATGCCACGGTGAATACG                   TTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTTTGTAACACCCGAAGTCGGTGGGGTAAC                   CCTTACGGGAGCCAGCCGCCGAAGGTGGGACAGATGATTGGGGTGAAGTCGTAACAAGGTAGCGTATCG                   GAAGGTGCGGTGGATCA (SEQ ID NO:74)                 Halobacillus     16S   NCIMB   GAGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGCGGGAAG         halophilus         2269   CAAGCGGATCCTTCGGGGGTGAAGCTTGTGGAACGAGCGGCGGACGGGTGAGTAACACGTGGGCAACCT                   GCCTGTAAGACCGGAATAACCCCGGGAAACCOGGGCTAATGCCGGATAACACCTACCTTCACCTGAAGG                   AAGGTTAAAAGATGGCTTCTCGCTATCACTTACAGATGGGCCCGCGGCGCATTAGCTAGTTGGTGAGGT                   AATAGCTCACCAAGGCGACGATGCGTAGCCGACCTGAGAOGGTGATCGGCCACACTGGGACTGAGACAC                   GGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGGAACG                   CCGCGTGAACGATGAAGGTCTTCGGATCGTAAAGTTCTGTTGTTAGGGAAGAACAAGTACCGTACGAAC                   ACAGCGGTACCTTGACGGTACCTAACGAGGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATAC                   GTAGGGGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGTTCTTTAAGTCTGATGT                   GAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGGAACTTGAGGACAGAAGAGGAGAGTGG                   AATTCCACGTGTAGCGGTGAAATGCGTAGATATGTGGAGGAACACCAGAGGCGAAGGCGACTCTCTGGT                   CTGTTTCTGACGCTGAGGTGCGAAAGCGTGGGTAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCG                   TAAACGATGAGTGCTAGGTGTTAGGGGGCTTCCACCCCTTAGTGCTGAAGTTAACGCATTAAGCACTCC                   GCCTGGGGAGTACGGCCGCAAGGNTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCA                   TGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTTGGAACCACCCTAGAGAT                   GGTGTTCCTTCGGGGACCAAGGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGG                   GTTAAGTCCCGCAACGAGCGCAACCCCTAATCTTAGTTGCCAGCATTCAGTTGGGCACTCTAAGGTGAC                   TGCCGGTGACAAACCGGAGGAAGGCGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACA                   CACGTGCTACAATGGATGGTACAAAGGGCAGCGAAGCCGCGAGGTGTAGCAAATCCCATAAAACCATTC                   TCAGTTCGGATTGCAGGCTGCAACTCGCCTGCATGAAGCCGGAATCGGTAGTAATCGCGGATCAGCATG                   CCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCACGAGAGTTGGCAACACCC                   (SEQ ID NO:75)                 Bacillus     16S   IAM12468   GACGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAGCGGAATGACGAGAGCTTGCTCTCGATTTTA         psychrophilus             GCGGCGGACGGGTGAGTAACACGTGGGCAACCTGCCCTACAGATGGGGATAACTCCGGGAAACCGGGGC                   TAATACCGAATAATCAGTTTGTCCGCATGGACAAACTCTGAAAGACGGTTTCGGCTGTCACTGTAGGAT                   GGGCCCGCGGCGCATTAGCTAGTTGGTGGGGTAATGGCCTACCAAGGCAACGATGCGTAGCCGACCTGG                   AGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATC                   TTCCACAATGGACGAAAGTCTGATGGAGCAATGCCGCGTGAGCGAAGAAGGTTTTCGGATCGTAAAGCT                   CTGTTGTAAGGGAAGAACACGTACGGGAGTAACTGCCCGTGCCATGACGGTACCTTATTAGAAAGCCAC                   CGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTA                   AAGCGCGCGCAGGCGGTTCTTTAAGTCTGATGTGAAAGCCCACGGCTCACCGTGGAGGGTCATTGGAAA                   CTGGAGAACTTGAGTACAGAAGAGGAAAGCGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGG                   AGGAACACCAGTGGCGAAGGCGGCTTTCTGGTCTGTAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCA                   AACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGGGGGTTTCCGCCC                   CTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGCCGCAAGGCTGAAACTCAAAGG                   AATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCA                   GGTCTTGACATCCCACTGACCGGTGTAGAGATACGCCTTTCCCTTCGGGGACAGTGGTGACAGGTGGTG                   CATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTT                   AGTTGCCAGCATTCAGTTGGGCACTCTAAGGTGACTGCCGGTGATAAACCGGAGGAAGGTGGGGATGAC                   GTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGATGATACAGAGGGTTGCCA                   ACCCGCGAGGGGGAGCCAATCCCATAAAATCGTTCCCAGTTCGGATTGGAGGCTGCAACTCGCCTCCAT                   GAAGTTGGAATCGCTAGTAATCGTGGATCAGCATGCCACGGTGAATACGTTCCCGGGTCTTGTACACAC                   CGCCCGTCACACCACGAGAGTTTGTAACACCCGAAGTCGGTGGGGTACATCTACGGGAGCCAGCCGCCG                   AAGGTGGGACAGATGATTGGGGTGAAGTCGTAACAA (SEQ ID NO:76)                 Paenibacillus     16S   ATCC   TTATTGGAGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGOCGTGCCTAATACATGCAAGTCGAGCGG         macerans         8244   ACCTGATGGAGTGCTTGCACTCCTGATGNNCGGCGGACGGGTGAGTAACACGTAGGCAACCTGCCCGTA                   AGACCGGGATAACTACCGGAAACGGTAGCTAATACCGGATAATCAAGTCTTCCGCATAGGAGACTTGGG                   AAAGGCGGAGCAATCTNTCACTTACGGANNNNNTNCGGCGCATTAGCTAGTTNGTGGGGTAACGGCTTA                   CCAAGGCGACGATGCGTAGCNGACCTGAGAGGGTGAACGGCCACACTGGGACTGAGACACGGCCCAGAC                   TCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAG                   TGATGAAGGTTTTCGGATCGTAAAGCTGNNTTGCCAGGGAAGAACGTCTTCTAGAGTAACTGCNANGAG                   AGTGACGGTACCTGAGAAGAAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCA                   AGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGCTGTTTAAGTCTGGTGTATAATCCCGG                   GGCTCAACTCCGGGTCGCACTGGAAACTGGACGGCTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTG                   TAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTNGGCTGTAACTGAC                   GCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAG                   TGCTAGGTGTTAGGGGTTTCGATACCCTTGGTGCCGAAGTAAACACATTAAGCACTCCGCCTGGGGAGT                   ACGGCCGCAAGGCTGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCAGTGGAGTATGTGGTTTAAT                   TCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCCTCTGACCGCTGTAGAGATATGGCTTTCTT                   CGGGACAGAGGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCG                   CAACGAGCGCAACCCTTGACTTTAGTTGCCAGCAAGTAAAGTTGGGCACTCTAGAGTGACTGCCGGTGA                   CAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTTGNCACACACGTACTAC                   AATGGCCGGTACAACGGGAAGCGAAGTAGTGATATGGAGCGAATCCTAGAAAGCCNGTCNCAGTTCGGA                   TTGCAGGCTGCAACTCGCCTGCATGAAGTCGGAATTGCTAGTAATCGCGGATCAGCATGCCGCGGTGCG                   AATACGTTCCCGGGTNTTGTACACACCGCCCGTCACACCACGAGAGTTTACAACACCCGAAGTCGGTGA                   GGTAACCGCAAGGGGCCAGCCGCCGAAGGTGGGGTAGATGATTGGGG (SEQ ID NO:77)                 Bacillus     16S   ATCC   AACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAATTGTTGAGTTTACTCAACAATTAGCGGCGG         psychrosaccha-         23296   ACGGGTGAGTAACACGTGGGCAACCTGCCTATAGACTGGATAACTTCGGGAACCGGAGCTAATACCGAT         rolyticus             ATGTTCTTCTCTCGCATGAGAGAAGATGGAAAGACGGTCTCGGCTGTCACTTATAGATGGGCCCGCGGC                   GCATTAGCTAGTTGGTGAGGTAATGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCG                   GCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGA                   CGAAAGTCTGACGGAGCAACGCCGCGTGAACGATGAAGGCTTTCOGGTCGTAAAGTTCTGTTGTTAGGG                   AAGAACAAGTACCAGAGTAACTGCTGGTACCTTGAGGTACCTAACCAGAAAGCCACGGCTAACTACGTG                   CCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGCAGGT                   GGTTCCTTAAGTCTGATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGGGGAACTTGA                   GTGTAGAAGAGGAAAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTG                   GCGAAGGCGACTTTCTGGTCTATAACTGACACTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGAT                   ACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGCCCTTTAGTGCTGCAG                   CTAACGCATTAAGCACTCCGCCTGGGGAGTACGGCCGCAAGGCTGAAACTCAAAGGAATTGACGGGGCC                   CGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCT                   CTGACACTCCTAGAGATAGGACGTTCCCCTTCGGGGGACAGAGTGACAGGTGGTGCATGGTTGTCGTCA                   GCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTC                   AGTTGGGCACTCTAAGGTGACTGCCGGTGATAAACCGGAGGAAGGTGGGGATGACGTCTCATCATGCCC                   CTTATGACCTGGGCTACACACGTGCTACAATGGATGGTACAAAGAGCTGCAAACCCGCGAGGGTAAGCG                   ATCTCATAAAGCCATTCTCAGTTCGGATTGCAGGCTGCAACTCGCCTGCATGAAGCCGGAATCGCTAGT                   AATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAG                   AGTTTGTAACACCCGAGTCGGTGAGGTAACCGCAAGGAGCCAGCCCGCCTAAGGTGGGACAGATGATTG                   GGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCA (SEQ ID NO:78)                 Bacillus     16S   ATCC   AACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAATGGATTAAGAGCTTGCTCTTATGAAGTTAG         mycoides         6462   CGGCGGACGGGTGAGTAACACGTGGGTAACCTACCCATAAGACTGGGATAACTCCGGGAAACCGGGGCT                   AATACCGGATAATATTTTGAACTGCATAGTTCGAAATTGAAAGGCGGCTTCGGCTGTCACTTATGGATG                   TGGACCCGCGTCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCGACGATGCGTAGCCGACCTG                   AGAGGGTGATCGGCCACACTGGGACTGAGAACGGCCCAGAGTCCTACGGGAGGCAGCAGTAGGGAATCT                   TCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGCTTTCGGGTCGTAAAACTC                   TGTTGTTAGGGAAGAACAAGTGCTAGTTGAATAAGCTGGCACCTTGAGCGCGCGCAGGTGGTTTCTTAA                   GTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGAGACTTGAGTGCAGAAGA                   GGAAAGTGGAATTCCATGTGTAGCGGTGAATGCGTAGAGATATGGAGGAACACCAGTGGCGAAGGCGAC                   TTTCTGGTCTGTAACTGACACTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGT                   CCACGCCGTAAACGATGAGTGCTGAAGTGTTAGAGGGTTTCCGCCCTTTAGTGCTGAAGTTAACGCATT                   AAGCACTCCGCCTGGGGAGTACGGCCGCAAGGCTGAAACTCAAGGAATTGACGGGGGCCCGCACAAGCG                   GTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGAAAACTC                   TAGAGATAGAGCTTCTCCTTCGGAGCAGAGTGAAGGTGGTGCATGGTTGTCGTCCTCGTGTCGTGAGAT                   GTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATTAGTTGCCATCATTAAGTTGGGCACTCTAAGGT                   GACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCT                   ACACACGTGCTACAATGGACGGTACAAAGAGCGCAAGACCGCGAGGTGGAGCTAATCTCATAAAACCGT                   CTCAGTTCGGATTGTAGGCTGCAACTCGCCTACATGAAGCTGGAATCGCTAGTAATCGCGGATCAGCAT                   GCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTTTGTAACACCCGA                   AGTCGGTGGGGTAACCTTTATGGAGCCAGCCGCCTAAGGTGGGACAGATGATTGGGGTGAATGCGTAAC                   AAGGTAGCCGTATCGGAAGGTGCGGCTGGATCA (SEQ ID NO:79)                 Bacillus     16S   DSM 485   GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGGACCAAAGGGAGCTTGCTCCCAGAGGTT         alcalophilus             AGCGGCGGACGGGTGAGTAACACGTGGNCAACCTGCCCTGTAGACTGGGATAACATCGAGAAATCGGTG                   CTAATACCGGATAATCAAAGGAATCACATGGTTCTTTTGTAAAAGATGGCTCCGGCTATCACTANGGGA                   TGGCCCGCGCGCATTAGCTAGTTGGTAAGGTAATGGCTTACCAAGGCGACGATGCGTAGCCGACCTGAG                   AGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTT                   CCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAGCTCT                   GTTGTTAGGGAAGAACAAGTGCCGNTCGAATAGGTCGGCACCTTGACGGTACCTAACCAGAAAGCCACG                   GCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAA                   AAGCGCGCGCAGGCGGTCTTTTAAGTCTGATGTGAAATATCGGGGCTCAACCCCGAGGGGTCATTGGAA                   ACTGGGAGACTTGAGTACAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGATATGTG                   GAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGCGCGAAAGCGTGGGGAGC                   AAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAGGTGTTAGGGGTTTCGATGC                   CCTTAGTGCCGAAGTTAACACATTAAGCACTCCGCCTGGGGAGTACGGCCGCAAGGCTGAAACTCAAAG                   GAATTGACGGGGGCCCGCACAAGCAGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACC                   AGGTCTTGACATCCTTTGACCACTCTAGAGATAGAGCTTTCCCCTTCGGGGGACAAAGTGACAGGTGGT                   GCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCT                   TAGTTGCCAGCATTTAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGA                   CGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACATGGATGGTACAAAGGGAGCGAC                   CGCGAGGTCGAGCCAATCCCATAAAGCCATTCTCAGTTCGGATTGTAGGCTGCAACTCGCCTACATGAA                   GCCGGAATTGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGC                   CCGTCACACCACGAGAGTTTGTAACACCCGAAGTCGGTGGGGTAACGTTTTGGAGCCAGCCGCCTAAGG                   TGGGACAGATGATTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTG (SEQ ID NO:80)                 Aneuriniba-     16S   ATCC   GAGAGTTTGATCCTGGCTCAGGNCGANCGCTGGCGGTGTGCCTAATACATGCAAGNCGAGCGGACCAAG         cillus         12856   GAAGAGCTTGCTCTTCGGCGGTTAGCGGCGGACGGGTGAGTAACACGTAGGCAGCCTGCCTGTACGACT         aneurinilyti-             GGGATAACTCCGTGAAACCGGAGCTAATACCAGATACGTTTTTCAGACCGCATGGTCTGAAAGAGAAAG         cus             ACCTCTGGTCACGTACAGATGGGCCTGCGGCGCATTAGCTAGTTGGTGGGGTAACGGTCTACCAAGGCG                   ACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGG                   GAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAACGATGAAG                   GTTTTCGGATCGTAAAGTTCTGTTGTTAGGGAAGAACCGCCGGGATGACCTCCCGGTCTGACGGTACCT                   AACGAGAAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGA                   ATTGGGCGTAAAGCGCGCGCAGGCGGCTTCTTAAGTCAGGTGTGAAAGCCCACGGNTCAACCGTGGAGG                   GCCACTTGAAACTGGGAGGCTTGAGTGCAGGAGAGGAGAGCGGAATTCCACGTGTAGCGGTGAAATGCG                   TAGAGATGTGGAGGAACAACCGTGGCGAAGGCGGCTCTCTGGCCTGTAACTGACGCTGGGGCGCGAAAG                   CGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGAAAACGTTGAGTGTTAGGTGTTGGGG                   ACTCCAATCCTCAGTGCCGCAGCTAACGCAATAAGCACTCCGCCTGGGGAGTACGGCCGCAAGGCTGAA                   ACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGA                   ACCTTGCCAGGGCTTGACATCCCGCTGTCCCTCCTAGAGATAGGAGNTCTCTTCGGAGCAGCGGTGACA                   GGTGGTGCATGGTTGTCGNCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCT                   TGTCCTTAGTTGNCAGCATTCAGTTGGGCACTCTAGGGAGACTGCCGTCGACAAGACGGAGGAAGGTGG                   GGATGACGTCAAATCATCATGCCCCTTATGTCCTGGGCTACACACGTGCTACAATGGATGGAACAACGG                   GCAGCCAACTCGCGAGAGTGCGCCAATCCCTTAAAACCATTCTCAGTTCGGATTGCAGGCTGCAACCTC                   GCCTGCATGAAGCCGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGTCTT                   GTACACACCGCCCGTCACACCACGAGAGTTTGCAACACCC (SEQ ID NO:81)                 Amphibacillus     16S   DSM 6626   ATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGCGTCNNATTAAAACAGA         xylanus             TCTCTTCGGAGTGACGTTTAATGGATCGAGCGGCGGATGGGTGAGTAACACGTGGCCAACCTGCCTATAA                   GACTGGGATAACTTACGGAAACGTGAGCTAATACCGGATAAAACCTTTTGTCTCCTGACAAGAGGATAAA                   AGATGGCGCAAGCTATCACTTATAGATGGGCCCGCGGCGCATTAGCTAGTTGGTGAGATAAAAGCTCACC                   AAGCCACGATGCGTAGCCGACCTGAGAGGGTGATTGGCCACACTGGGACTGAGATACGGCCCGATCCTAC                   GGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAACGAAGAA                   GGTCTTCGGATCGTAAAGTTCTGTTGTTAGGGAAGAACACGTACCATTCGAATAGGGTGGTACCTTGACG                   GTACCTAACGAGAAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGT                   CCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGTTCTTTAAGTCTGATGTGAAATCTTGCGGCTCAACC                   GCAAGCGGTCATTGGAAACTGGAGAACTTGAGGACAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGA                   ATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGCGCG                   AAAGCGTGGGTAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAGGTGTTA                   GGGGGTTTCCCCCCCTTAGTGCTGGCGTTAACGCATTAAGCACTCCNCCTGGGGAGTACGGCCGCAAGGC                   TGAAACTCAAAAGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGA                   AGAACCTTACCAGGTCTTGACATCCCGCTGACCGCTATGGAGACATAGCTTTCCCTTCGGGGACAGCGGT                   GACAGGTGGTGCATGGTTGTCGTTGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACC                   CTTGAACTTAGTTGCCAGCATTCAGTTGGGCACTCTAAGTTGACTGCCGGTGACAAACCGGAGGAAGGTT                   GGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGNTTGGTAGTTCG                   GATTGTCGGTTGAACTCGCCTACATGAAGCCGGAATCGCTAGTAATCGCGGATCAGAATGCCGCGGTGAA                   TACGTTCCCGGGTCTTGTACACACCGTCCGTCACACCACGAGAGTTAGCAACACCCGAAGTCGGTGAGGT                   AACGCTTTTAGNGAGCCAGCCGCCGAAGGTGGGGCCAATGATTGGGGTGAAGTCGTAACAAGGTAGCCGT                   ATCGGAAGGTGCGGNTGGATCACCTCCTT (SEQ ID NO:82)                 Bacillus     16S   IAM   GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGCGGGAACNAAGCAGATCTCCTTCGGGGGT         pantothenticus         11061   GACGCTTGTCCAACGGACGGGTGAGTAACACGTGGGCAACCTACCTGTAAGACTGGGATAACTCCGGGAA                   ACCGGGGCTAATACCGGATGATACATATCGTCCATACGAGATGTTGAAAAGGCGGCATATGCTGTCACTT                   ACAGATGGGCCCGCGGCGCATTAGCTAGTTGGTGAGATAAAAGCTCACCAAGGCGACGATGCGTAGCCGA                   CCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGA                   ATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAA                   CTCTGTTGTTAGGGAAGAACAAGTGCCATTCGAATAGGTTGGCACCTTGACGGTACCTAACCAGAAAGCC                   CCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTGTCCGGAATTATTGGGCGTA                   AAGCGCGCGCAGGCGGTCCTTTAAGTCTGATGTGAAAGCCCACGGCTTAACCGTGGAGGGCCATTGGAAA                   CTGGGGGACTTGAGTACAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGA                   GGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGTGCGAAAGCGTGGGTAGCGAA                   CAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAGGTGTTAGGGGGTTTCCGCCCCTT                   AGTGCTGAAGTTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATT                   GACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCT                   TGACATCCTCTGACGCCCCTAGAGATAGGGAGTGATCTTAGTTGCCAGCATTTAGTTGGGCACTCTAAGG                   TGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCT                   ACACACGTGCTACAATGGATGGAACAAAGGGCAGCGAAGCCGCGAGGCCAAGCAAATCCCATAAAACCAT                   TCTCAGTTCGGATTGCAGGCTGCAACTCGCCTGCATGAAGCCGGAATCGCTAGTAATCGCGGATCAGCAT                   GCCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCACGAGAGTTGGTAACACCCGAA                   GTCGGTGAGGTAACCTTTTGGAGCCAGCCGCCGAAGGTGGGACTAATGATTGGGGTGAAGTCGTAACAA                   (SEQ ID NO:83)                 Paenibacillus     16S   ATCC   TGCCTAATACATGCAAGTCGAGCGGACTCAACTGTTTCCTTCGGGAAACCGTTAGGTTAGCGGCGGACGG         popilliae         14706   GTGAGTAATACGTAGGTAACCTGCCCTTAAGACYGGGATAACTCACGGAAACGTGGGCTAAWACCGGATA                   GGCGATTTGCTCGCATGAGGGAATCGGGAAAGGCGGAGCAATCTGCCACTTATGGATGGACCTACGGCGC                   ATTAGCTAGTTGGTGRGGTAACGGCTCACCAAGGCGACGATGCGTAGCCGACCTGAGAGGGTGATCGGCC                   ACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGCA                   AGTCTGACGGAGCAACGCCGCGTGAGTGATGAACGTTTTCGGATCGTAAAGCTCTGTTGCCAGGGAAGAA                   CGCTATGGAGAGTAACTGTTCCATAGGTGACGATACCTGAGAAGAAAGCCCCGGCTAACTACGTGCCAGC                   AGCCGCGGTAATACGTAGCGGGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGTCA                   TGTAAGTCTGGTGTTTAAACCCGGGGCTCAACTCCGGGTCGCATCGGAAACTGTGTGACTTGAGTGCAGA                   AGAGGAAAGTGGAATTCCACGTGTAGCGGTGATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGA                   CTTTCTGGGCTGTAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAACAGGATTAGATACCCTGGTAGTC                   CACGCCGTAAACGATGAATGCTAGGTGTTAGGGGTTTCGATACCCTTGGTGCCGAAGTTAACACATTAAG                   CATTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCAGTG                   GAGTATGTGGTTTAATTCGAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCCTCTGACCGCGCTAGA                   GATAGGGCTTCCCTTCGGGGCAGAGGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTOTCGTGAGATGT                   TGGGTTAAGTCCCGCAACGAGCGCAACCCTTAACTTTAGTTGCCAGCATTGAGTTGGGCACTCTAGAGTG                   ACTGCCGGTGAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACAC                   ACGTACTACAATGGCTGGTACAACGGGAAGCGAAGCCGCGAGGTGGAGCGAATCCTAAAAAGCCAGTCTC                   AGTTCGGATTGCAGGCTGCAACTCGCCTGCATGAAGTCGGAATTGCTAGTAATCGCGGATCAGCATGCCG                   CGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCACGAGAGTTTACAACACCCGAAGTCG                   GTGGGGTAACCGCAAGGAGCCAGCCGCCGAAGGTGGGGTAGATGATTGGGGTGAAGTCGTAACAA                   (SEQ ID NO:84)                 B. cereus     cpn60       GCAACTGTATTAGCGCAAGCTATGATTCGTGAAGGTCTTAAAAACGTAACAGCTGGTGCGAACCCAATGG                   GGCTTCGTAAAGGTATCGAAAAAGCTGTTACTGCTGCAATTGAAGAATTAAAAACGATTTCTAAACCAAT                   CGAAGGCAAATCTTCTATCGCACAAGTAGCTGCTATTTCTGCAGCTGACGAAGAGTAGGTCAATTAATCG                   CTGAAGCAATGGAGCGCGTTGGTAACGACGGCGTTATTACTTTAGAAGAGTCTAAAGGATTCACAACAGA                   ATTAGACGTAGTAGAAGGTATGCAATTTGATCGTGGATATGCATCTCCTTACATGATTACTGATTCTGAC                   AAAATGGAAGCAGTTCTTGATAACCCATACATCTTAATTACTGACAAAAAGATTTCTAACATTCAAGAAA                   TCTTACCAGTATTAGAGCAAGTGGTACAACAAGGTAAACCACTTCTTATCATTGCTGAAGATGTAAAAGG                   CGAAGCTTTAGCTACATTAGTAGTGAACAAACTTCGTGGTACATTCAACGTAGTAGCTGTT                   (SEQ ID NO:85)                 .thuringien-     Cpn60       GCAACTGTATTAGCGCAAGCTATGATTCGTGAAGGTCTTAAAAACGTAACAGCTGGTGCGAACCCAATGG         sis  var.           GTCTTCGTAAAGGTATCGAAAAAGCTGTTACTGCTGCAATTGAAGAATTAAAAACGATTTCTAAACCAAT         kurstaki             CGAAGGTAAATCTTCTATCGCACAAGTAGCTGCTATTTCTGCTGCTGACGAAGAAGTAGGTCAATTAATC       HD1 (=B51  B.             CTGAAGCAATGGAGCGCGTTGGTAACGACGGCGTTATTACTTTAGAAGAATCTAAAGGCTTCACAACAGA         anthracis )           ATTAGACGTAGTAGAAGGTATGCAATTTGATCGTGGATATGCATCTCCTTACATGATTACTGATTCTGAC                   AAAATGGAAGCAGTTCTTGATAACCCATACATCTTAATCACTGACATAAGATTTCTAACATTCAAGAAAT                   CTTACCAGTATTAGAGCAAGTGGTACAACAAGGTAAACCACTTCTTATCATTGCTGAAGATGTAGAGGCG                   AGCGTTAGCTACATTAGTAGTGAACAAACTTCGTGGTACATTCAATGTAGTAGCTGTT                   (SEQ ID NO:86)                 B. subtilis     cpn60       GCAACAGTTCTTGCGCAAGCAATGATCCGTGAAGGCCTTAAAAACGTAACAGCAGGCGCTAACCCTGTAG       168           GCGTGCGTAAAGGGATGGAACAAGCTGTAGCGGTTGCGATCGACTTAGAAATTTCTAAGCCAATCGAAGG                   CAAAGAGTCTATCGCTCAGGTTGCTGCGATCTCTGCTGCTGATGAGGAAGTCGGAAGCCTTATCGCTGAA                   GCAATGGAGCGCGTAGGAAACGACGGCGTTATCACAATCGAAGAGTCTAAAGGCTTCACAACTGAGCTTG                   ACTGAGCTTGAAGTTGTTGAAGGTATGCAATTCGACCGCGGATATGCGTCTCCTTACATGGTAACTGACT                   CTGATAAGATGGAAGCGGTTCTTGACAATCCTTACATCTTAATCACAGACAAAAAAATCACAAACATTCA                   AGAAATCCTTCCTGTGCTTGAGCAGGTTGTTCAGCAAGGCAAACCATTGCTTCTGATCGCTGAGGATGTT                   GAAGGCGAAGCACTTGCTACACTTGTTGTGAACAAACTTCGCGGCACATTCAACGCAGTGGCTGTT                   (SEQ ID NO:87)                 B. subtilis     cpn60       GCGACAGTTCTTGCGCAAGCAATGATCCGTGAAGGCCTTAAAAACGTAACAGCAGGCGCTAATCCTGTAG       W235R           GCGTTCGTAAAGGTATGGAAAAAGCTGTAGCGGTTGCGATCGAAAACTTAAAAGAAATTTCTAAGCCAAT                   CGAAGGCAAGGAGTCTATCGCTCAGGTTGCTGCGATCTCTGCTGCTGAGGAGGAAGTCGGAAGCCTTATC                   GCTGAAGCAATGGAGCGCGTAGGCAACGACGGCGTTATCACAATCGAAGAGTCTAAAGGCTTCACAACTG                   AGCTTGAAGTTGTTGAAGGTATGCAATTCGACCGCGGATATGCGTCTCCTTACATGGTAACTGACTCTGA                   TAAGATGGAAGCGGTTCTTGACAATCCTTACATCTTAATCACAGACAAAAAAATCACAAACATTCAAGAA                   ATCCTTCCTGTACTTGAGCAGGTTGTTCAGCAAGGCAAACCATTGCTTCTAATCGCTGAGGATGTTGAAG                   GCGAAGCACTTGCAACACTTGTTGTGAACAAACTTCGCGGTACATTCAACGCAGTTGCTGTT                   (SEQ ID NO:88)                 B. licheni-     cpn60   ATCC14580   GCGACAGTTCTAGCTCAGGCGATGATTCGCGAAGGTCTTAAAAACGTAACTGCCGGCGCTAACCCTGTAG         formis             GCGTGCGTATCGAGCAGGCTGTGGCTGTAGCTGTTGAAAGCCTGAAAGAAATCTCTAAACCAATTGAAGG                   CAAAGAATCAATCGCACAAGTTGCTTCAATCTCCGCTGCAGACGAAGAAGTCGGAAGCCTGATCGCTGAA                   GCAATGGAGCGCGTCGGCAACGACGGTGTTATCACGATCGAAGAATCCAAAGGATTCACAACAGAGCTTG                   AAGTGGTTGAAGGTATGCAGTTCGACCGCGATATGCGTCTCCTTACATGGTGACGGATTCCGATAAGATG                   GAAGCGGTTCTTGAGAATCCGTACATCTTAGTAACAGACAAAAAAATCACAAACATTCAAGAAATCCTGC                   CGGTGCTTGAGCAAGTCGTGCAACAAGGCAAACCGTTGCTTCTGATTGCTGAAGACGTTGAAGGTGAAGC                   TCTTGCAACATTGGTTGTCCAAGCTTCGCGGAACATTCAACGCAGTGGCTGTT (SEQ ID NO:89)                 B. pumilus     cpn60       GCGACTGTACTTGCGCAGGCTATGATCCGCGAAGGCCTTAAAAACGTAACTGCGGGGGCTAACCCTGTCG       B205-L M&amp;G           GCGTGCGTAAAGGTATGGAACA AGCCGTGACTGTAGCAATCGAAAACTTAAAAGAAATTTCTAAGCCGA                   TCGAAGGCGAGTCTATCGCTCAGGTTGCTGCGATCTCTGCTGCTGATGAGGAAGTCGGAAGCCTTATCGC                   TGAAGCAATGGAGCGCGTAGTAAACGACGGCGTCATCACAATCGAAGAGTCTAAAGGTTTCACA                   ACTGAGCTTGAAGTTGTTGAAGGTATGCAATTCGACCGCGATATGCGTCTCCTTACATGGTGACTGACTC                   TGATAAGATGGAAGCGGTTCTTGACAATCCTTACATCTTAATCACAGACAAAAAAATCACAAACATTCAA                   GAAATCCTTCCTGTGCTTGAGCAAGTTGTACAGCAAGGCAAACCATTGCTTCTGATCGCTGAAGATGTTG                   AAGGGGAAGCTCTTGCTACACTCGTTGTCAACAAACTTCGCGGCACATTCAACGCTGTTGCCGTT                   (SEQ ID NO:90)                 B. pumilus     cpn60   ATCC7061   GCAACAGTTCTAGCTCAAGCGATGATCCGTGAAGGTCTTAAAAACGTAACAGCTGGTGCAAACCCTGTTG                   GCGTTCGTAAAGGGTATCGAAGAAGCCGTGACTGTAGCAATCGAAAACTTAAAAGAAATTTCTAAGCCGA                   TCGAAGGCGTTCGTAAAGGGATCGAAGAAGTTGGAAGCCTGATCGCTGAAGCAATGGAGCGTGTAGGTAA                   CGACGGCGTGATCACAATCGAAGAATCTAAAGGGTTCACAACTGAGCTTGAAGTGGTTGAAGGATGCAGT                   TTGACCGAGGATATGCTTCACCATACATGGTGACGCTGATAAGATGGAAGCGGTTCTTGAAAATCCTTAC                   ATCTTAATCACTGATAAAAAAATCACAAACATTCAAGAAATCCTTCCTGTACTTGAGCAAGTTGTACAAC                   AAGGAAAACCATTATTGCTCATTGCTGAAGATGTAGAAGGCGAAGCACTTGCAACACTTGTTGTGAACAA                   ACTTCGTGGAACATTCAACGCAGTGGCAGTA (SEQ ID NO: 91)                         B. amylolique-     cpn60       GCGACTGTGCTTGCACAGGCTATGATCCGCGAAGGCCTTAAACGTAACTGCGGGAGCTAATCCTGTCGGC         faciens  H           GTGCGTAAAGGTATGGAACAAGCCGTAACCGTGGCAATCGAAAACTTAAAAGAAATTTCTAAGCCGATCG                   AAGGCAAAGAGTCTATCGCTCAGGTTGCTGCAATCTCTGCTGCTGATGAGGAAGTCGGAAGCCTTATCGC                   TGAAGCAATGGAGCGCGTAGGAAACGACGGCGTTATCACAATCGAAGAGTCTAAAGGCTTCACAACTGAG                   CTTGAAGTGGTTGAAGGTATGCAATTCGACCGCGGATATGCGTCTCCTTACATGGTTGACTGACTCTGAT                   AAGATGGAAGCGGTTCTTGATAATCCTTACATCTTAATCACAGACAAAAAAATCACAAACATTCAAGAAA                   TCCTTCCTGTGCTTGAGCAAGTTGTACAGCAAGGCAAACCATTGCTTCTGATCGCTGAAGATGTTGAAGG                   TGAAGCTCTTGCTACACTCGTTGTCAACAAACTTCGCGGCACATTCAACGCTGTTGCCGTT                   (SEQ ID NO:92)                 B. amylolique-     cpn60       GCAACTGTATTAGCACAAGCTATGATTCGTGAAGGTCTTAAAAACGTAACAGCTGGTGCGAACCCAATGG         faciens  N           GTCTTCGTAAAGGTATCGAAAAAGCTGTAGTTGCTGCAGTAGAAGAATTAAAAACGATTTCTAAACCAAT                   CGAAGGTAAATCTTCAATCGCACAAGTAGCTGCTATTTCTGCGGCTGACGAAGAAGTAGGTCTTTAATC                   GCTGAAGCAATGGAGCGCGTTGGTAACGACGGCGTTATTACTTTAGAAGAATCTAAAGGATTCACAACAG                   AATTAGATGTAGTAGAAGGTATGCAATTTGATCGTGGATATGCATCTCCTTACATGATTACTGATTCTGA                   CAAAATGGAAGCAGTTCTTGATAACCCATACATCTTAATCACTGACAAAAAGATTTCTAACATTCAAGAA                   ATCTTACCAGTATTAGAGCAAGTGGTACAACAAGGTAAACCGCTTCTTATCATTGCTGAAGATGTAGAAG                   GCGAAGCATTAGCTACATTAGTAGTGAACAAACTTCGTGGTACATTCAATGTAGTAGCTGTT                   (SEQ ID NO:93)                 B. globigii     cpn60       GCTACAGTTCTTGTTCAGGCTATGATTCGTGAAGGTCTTAAAAACGTAACGGCAGGCGCTAACCCTGTAG       SB512           GCGTTCGTAAAGGTATGGAACAAGCTGTAACAGTTGCGATTCAAACCTTCAAGAAATCTCTAAACCGATC                   GAAGGAAAAGAGTCTATCGCTCAGGTTGCTGCGATTTCTGCTGCTGATGAAAAAGTCGGAAGCCTGATTG                   CTGAAGCGATGGAGCGCGTTGGAAACGACGGCGTTATCACGATCGAAGAATCTAAAGGTTTCACAACTGA                   GCTTGAAGTTGTTGAAGGTATGCAGTTCGACCGCGGATATGCATCTCCTTACATGGTAACTGATTCTGAT                   AAGATGGAAGCGGTTCTTGAAAATCCTTACATCTTAATCACAGACAAAAAAATTACAAATATTCAAGAAA                   TCCTTCCTGTGCTTGAGCAGGTTGTTCAGCAAGGCAAACCATTGCTTCTGATTGCTGAGGATGTTGAAGG                   TGAAGCTCTTGCAACACTTGTTGTGAACAAACTTCGCGGCACATTCAACGCAGTTGCCGTT                   (SEQ ID NO:94)                 G. stearother-     cpn60       GCAACAGTTTTAGCGCAAGCAATGATCCGCGAAGGATTGAAAAACGTTACAGCTGGCGCTAACCCAATGG         mophilus             GCATCCGTAAAGGTATTGAAAAAGCGGTCGCTGTGGCAGTAGAAGAATTAAAAGCAATCTCCAAACCAAT       BGSC strain           TCAAGGTAAAGAATCGATTGCTCAAGTTGCAGCGATCTCTGCGGCTGACGAAGAAGTTGGTCAATTAATC       9A2           GCAGAAGCAATGGAACGCGTTGGCAACGATGGTGTTATCACATTAGAAGAATCGAAAGGCTTCGCAACGG                   AATTAGATGTTGTCGAAGGTATGCAATTTGACCGTGGTTATGTATCTCCATACATGATCACAGATACAGA                   AAAAATGGAAGCAGTGCTTGAAAATCCATACATCTTAATTACAGATAAAAAAGTTTCTAGCATCCAAGAA                   ATCTTGCCTATCTTAGAACAAGTAGTTCAACAAGACCGCTATTAATTATCGCAGAAGATGTCGAAGGCGA                   AGCGCTCGCAACATTAGTCGTCAACAAACTTCGTGGTACATTCAATGCGGTAGCGGTA                   (SEQ ID NO:95)                 B. megaterium     cpn60       GCAACAGTTTTAGCGCAAGCAATGATCAGAGAAGGTCTTAAAAACGTAACGGCTGGTGCTAACCCAATGG       899           GTATCCGTAAAGGTATGGAAAAGGCAGTAGCTGTAGCGGTTGAAGAACTAAAAGCAATCTCTAAACCAAT                   TCAAGGTAAAGATTCAATTGCTCAAGTAGCGGCTATCTCAGCAGCTGACGAAGAAGTAGGTCAATTAATT                   GCTGAAGCAATGGAGCGCGTTGGTAACGACGGCGTTATCACACTTGAAGAATCAAAAGGTTTCACAACTG                   AATTAGAAGTGGTAGAAGGTATGCAGTTTGACCGTGGATATGCATCTCCTTACATGGTAACTGATTCAGA                   TAAAATGGAAGCTGTATTAGATGATCCATACATCTTAATCACAGACAAAAAAATCGGTAACATTCAAGAA                   ATCTTACCGGTATTAGAGCAAGTTGTTCAACAAGGCAAGCCTCTATTGATCATCGCTGAAGACGTAGAAG                   GCGAAGCTTTAGCAACATTAGTTGTGAACAAACTTCGTGGTACATTCACAGCTGTAGCTGTT                   (SEQ ID NO:96)                 B. megaterium     cpn60   ATC19213   GCAACTGTATTAGCGCAAGCTATGATTCGTGAAGGTCTTAAAAACGTAACAGCTGGTGCTAACCCAATGG       bgsc           GTCTTCGTAAAGGTATCGAAAAAGCTGTTACTGCTGCAATTGAAGAATTAAAAACGATTTCTAAACCAAT                   CGAAGGCAAATCTTCTATCGCACAAGTAGCTGCTATTTCTGCAGCTGACGAAGAAGTAGGTCAATTAATC                   GCTGAAGCAATGGAGCGCGTTGGTAACGACGGCGTTATTACTTTAGAAGAGTCTAAAGGATTCACAACAG                   AATTAGACGTAGTAGAAGGTATGCAATTTGATCGTGGATATGCATCTCCTTACATGATTACTGATTCTGA                   CAAAATGGAAGCAGTTCTTGATAACCCATATATCTTAATTACTGACAAAAAGATTTCTAACATTCAAGAA                   ATCTTACCAGTATTAGAGCAAGTGGTACAACAAGGTAAACCACTTCTTATCATTGCTGAAGATGTAGAAG                   GGCGAAGCTTTAGCTACCATTAGTAGTGAACAAACTTCGTGGTCATTCAATGTAGTAGCTGTT                   (SEQ ID NO:97)                 B. coagulans     cpn60       GCGACCGTTCTGGCCCAGGCAATGATCCGTGAAGGCCTGAAAAACGTAACAGCAGGCGCAAACCCGGTTG       CECT12           GCATCCGCAAAGGGATTGAAAAAGCGGTTGCGGCTGCTGTTGAAGAATTAAAAGCCATTTCGAAACCAAT                   CGAAGGCAAAGCTTCCATCGCCCAAGTTGCTGCAATTTCCTCTGCTGACGAAGAAGTTGGCGAATTGATC                   GCTGAAGCAATGGAACGCGTGGGCAACGACGGCGTCATTACCATTGAAGAATCAAAAGGCTTCTCAACGG                   AATTGGACGTTGTGGAAGGGATGCAGTTTGACCGTGGCTATGCATCGCCTTACATGGTAACGGATTCCGA                   CAAAATGGAAGCTGTTCTGGATAACCCTTATATCTTAATTACAGACAAGAAGATTTCCAATATCCAGGAA                   ATCCTCCCTGTTCTCGAACAAGTTGTCCAACAAGGCAAACCGCTGTTGCTGATTGCGGAAGATGTTGAAG                   GGGAAGCTCTTGCAACACTCGTTGTCAACAAACTGCGTGGCACATTCAATGCAGTTGCGGTG                   (SEQ ID NO:98)                 A. acidocal-     cpn60       GCGACGGTGCTGGCGCAGGCGATGATCCGCGAGGGTCTGAAGAACGTCGCCGCTGGTGCGAACCCGATGG         darius             TGCTCCGCCGCGGCATTGAGAAGGCCGTGACGGCTGCGGTCGAGGAGCTGAAGAAGATCGCGAAGCCGGT       CECT4328           CCAGGGCCGCAAGAACATCGCGGAGGTTGCCGCCATCTCGGCTGGTTCGAACGAAATCGGCGAGCTCATC                   GCGGATGCGATGGAGAAGGTTGGCAACGACGGCGTGATCACCGTCGAAGAGTCGAAGGGCTTCACGACCG                   AGCTTGAGGTCGTCGAGGGTATGCAGTTCGACCGCGGCTACATCTCGCCGTACATGGTGACGGACGCGGA                   CAAGATGGAGGCTGTGCTGGACGAGCCGCTCATCCTCATCACCGACAAGAAGGTCTCGAGCATCCAGGAG                   ATCCTGCCGGTGCTGGAGCGCGTCGTGCAGGCTGGCCGTTCGCTGCTCCTCATCGCCGAGGATGTGGAGG                   GCGAAGCGCTCGCGACGCTCGTGGTCAACAAGATCCGCGGTACGTTCAACGCCGTGGCCGTCAAA                   (SEQ ID NG:99)                 B. lentus     cpn60       GCAACTGTTCTTGCACAAGCAATGATCCGTGAAGGCTTGAAAAACGTAACTGCTGGAGCTAATCCTGTTG       CECT 18           GCGTTAAAAAAGGGATGGAAAAAGCAGTTGCAACAGCAGTAACTGAGCTACAAACTATCTCAAAACAAAT                   TGAAGATAAAGAATCAATTGCTCAAGTTGCATCTATTTCTTCTGGTGACGAAGAAGTTGGCCAATTAATA                   GCTGAAGCAATGGAACGTGTTGGTAATGATGGCGTTATTACAATTGAAGAGTCTCGTGGTTTCACTACAG                   AGCTTGAAGTTGTAGAAGGAATGCAGTTCGACCGTGGTTATGCATCTCCTTATATGGTAACAGATTCTGA                   TAAAATGGAAGCTGTGCTTGAAAATCCATATATCTTGATCACAGATAAGAAAATTACAAACATCCAAGAA                   GTACTACCTGTTCTTGAGCAAGTTGTTCAACAAGGTAAACCATTGTTGATGATTGCTGAAGATGTAGAAG                   GTGAAGCACTTGCTACACTTGTAGTAAACAAACTTCGCGGAACATTCAACGCAGTAGCTGTT                   (SEQ ID NO:100)                 H. halophilus     cpn60       GCAACCGTACTAGCGCAAGCGATGATCCGTGAAGGTCTAAAAAACGTAACATCCGGTGCGAACCCAGTAG                   GCATTCGCCGCGGAATTGAAAAAGCAACCGAAGTCGCTACTCAGGAACTTCGCAAAATCTCTAAGCCAAT                   CGAAGGCCGCGAGTCCATTTCTCAGGTAGCTTCCATCTCTGCTTCCGATAACGAAGTCGGCCAGCTGATT                   GCTGAAGCGATGGAGCGCGTAGGAAACGATGGCGTTATTACAATTGAAGAATCTAAAGGTTTCAATACAG                   AACTAGAAGTGGTTGAAGGTATGCAGTTCGACCGCGGCTATGCTTCTCCATACATGGTTACAGACCAGGA                   TAAAATGGAAGCGGTTCTTGATGATCCTTACATTCTAATTACGGATAAGAAAATCAACAACATCCAGGAA                   GTACTTCCTGTACTTGAGCAAGTGGTACAGCAATCCAAGCCGTTGCTACTGATCTCTGAAGACGTAGAAG                   GCGAAGCACTTGCTACACTTGTTGTGAACAAACTGCGCGGTACATTCAACGCTGTATCCGTT                   (SEQ ID NO:101)                 B. marinus     cpn60       GCAACTGTTCTTGCTCAAGCAATGATCCGTGAAGGTCTTAAAAACGTTACAGCTGGTGCAAACCCAGTTG                   GCGTTCGTAAAGGAATTGAAAAAGCGGTTCAATCAGCACTTGTTGAGCTTAAAGAGATCTCAAAACCGAT                   TGAAGGCAAAGAGTCGATTGCACAAGTTGCAGCTATCTCTTCATCAGATGAAGAAGTAGGGCAATTGATT                   GCTGAAGCAATGGAGCGCGTTGGTAACGATGGCGTGATTACAATCGAAGAATCAAAAGGCTTCACAACTG                   ACTGGATGTAGTAGAAGGTATGCAATTTGACCGTGGATATGCATCACCGTACATGGTAACAGATTCAGAT                   AAAATGGAAGCAGTTTTAGAAAATCCATATATCTTAATCACAGACAAGAAAATCGGTAACATCCAAGAAG                   TGCTTCCTGTACTTGAGCAAGTTGTACAACAAGGTAAGCCACTATTGATTGTTGCTGAAGATGTTGAAGG                   CGAAGCACTAGCAACACTTGTTGTGAACAAACTACGTGGAACATTCAACGCAGTAGCTGTC                   (SEQ ID NO:102)                 S. psychrophila     cpn60       GCAACAGTTCTAGCGCAAGCAATGATCCGTGAAGGACTGAAAAACGTAACTGCAGGTGCTAACCCTGTC       CECT4073           GGAATCCGTAAAGGAATCGAAAAAGCGGTTATAGCTGCTGTTGAAGGCCTTCAAGAATCTCCAATGAAA                   TCGAAGGAAAAGAAGAGATTGCACAAGTCGCATCTATTTCTTCTGGAGACGAAGAAGTTGGGAAACTTA                   TTGCTGAAGCAATGGAGCGCGTTGGCAACGATGGTGTCATTACTATCGAAGAGTCAAAAGGCTTCACGA                   CTGAACTAGACGTTGTTGAAGGAATGCAATTTGACCGCGGTTATGCATCTGCATACATGGCAACGGATA                   CAGACAAAATGGAAGCAGTTTTGGACAATCCGTATATCTTGATCACAGATAAAAAGATTACGAACATCC                   AAGAAATTCTTCCTGTTCTTGAGCAAGTAGTTCAACAAGGTAAGCCACTTCTTATGATCGCAGAAGACG                   TTGAAGGCGAAGCACTTGCAACACTTGTTGTGAACAAACTACGTGGTACATTCAATGCTGTTGCTGTT                   (SEQ ID NO:103)                 P. macerans     cpn60       GCAACAGTTCTTGCTCAGGCAATGATCCGTGAAGGCCTTAAGAACGTAACTGCAGGTGCTAACCCAATGG       CECT19           GCATCCGCAAAGGAATTGAAAAAGCGGTTTCTACTGCTGTTGAAGAGTTAAAAGCTATTTCAAAACCTAT       (= B58           CGAAAACAAAGAATCTATCGCACAGGTTGCTGCTATTTCTGCTGCTGACAATGAAGTTGGCCAGCTGATC         B. firmus             GCTGAAGCAATGGAGCGCGTTGGCAACGATGGTGTTATCACAATCGAAGAATCTAAAGGTTTCACAACTG                   AGCTTGATGTGGTAGAAGGTATGCAATTCGACCGCGGATACGCTTCACCATACATGGTTACAGATTCTGA                   TAAGATGGAAGCGGTTCTTGAAAACCCTTATATCTTAATCACTGATAAGAAGATCACAAGCATCCAGGAA                   ATTCTTCCTGTACTTGAGCAGGTTGTACAGCAAGGCAAGCCTTTATTGCTTGTAGCTGAGGATGTTGAAG                   GTGAAGCACTAGCTACATTAGTAGTGAATAAGCTTCGTGGAACTTTCAACGCTGTAGCGGTT                   (SEQ ID NO:104)                 B. psychro-     cpn60       GCTACTGTCCTTGCACAAGCTATGATTCGTGAAGGCCTGAAAAACGTAACGGCTGGCGCGAATCCTATGG         saccharolyticus             GCATTCGTAAAGGGATTGAAAAAGCTGTGAAAGCTGCAATTAGTGAGTTACAAGCTATCTCTAAACCAAT       CECT           CGAAAACAAAGAGTCTATTGCACAAGTTGCAGCAATCTCAGCTTCTGACGAAGAAGTGGGTCAATTAATT       4074           GCTGAAGCAATGGAACGCGTTGGCAACGACGGTGTTATCACAATTGAAGAGTCTAAAGGATTCTCAACTG                   AATTGGACGTAGTAGAAGGTATGCAGTTCGACCGTGGATATGCATCTGCTTATATGGTAACAAACCCAGA                   TAAAATGGAAGCAGTTCTTGAAAATCCATATATCTTAATTACTGACAAAAAAATCTCAAACATTCAAGAA                   ATTCTTCCTGTACTTGAACAAGTTGTTCAACAAGGAAAATCTCTATTGCTAATTGCTGAAGACATTGAAG                   GCGAAGCACTATCAACACTTGTTGTGAACAAACTTCGTGGAACATTCAATGCAGTTGCTGTA                   (SEQ ID NO:105)                 B. mycoides     cpn60       GCAACTGTATTAGCGCAAGCTATGATTCGTGAAGGTCTTAAAAACGTAACAGCTGGTGCAAACCCAATGG       CECT 4128           GTCTTCGTAAAGGTATCGAAAAAGCTGTTACTGCTGCAATTGAAGAATTAAAAGCGATTTCTAAACCAAT                   CGAAGGTAAATCTTCTATCGCACAAGTAGCTGCTATTTCTTCGGCTGACGAGAAGTAGGTCAATTAATC                   GCTGAAGCAATGGAGCGCGTTGGTAACGACGGCGTTATTACTTTAGAAGAATCTAAAGGATTCACAACAG                   AATTAGACGTAGTAGAAGGTATGCAATTTGATCGTGGATAAGCATCTCCTACATGATTACTGATTCTGAC                   AATGAGAGTTCACTTCTTATCATTGCTGAAGATGTAGAAGGCGAAGCGTTAGCTACATTAGTAGTGAACA                   AACTTCGTGGTACATTCAATGTAGTTGCTGTT (SEQ ID NO:106)                 B. alcalophilus     cpn60       GCGACTGTTCTAGCTCAAGCGATGATTCGTGAAGGTCTTAAAAACGTAACATCTGGTGCGAACCCAATGG       CECT 1           GTATCCGTAAAGGGATTGAAAAAGCAACAGCTGCTGCGGTTACAGAACTTAAAAATATTGCGAAACCAAT                   CGAAGGCAAAGAGTCAATCGCACAAGTTGCGGCTAACTCAGCAGCTGACGAAGAAGTTGGACAAATTATC                   GCAGAAGCAATGGAACGTGTTGGAAACGACGGCGTTATTACAATCGAAGAATCAAAAGGTTTCTCTACTG                   AATTAGAAGTAGTAGAAGGTATGCAATTCGATCGTGGTTTCGTTTCTCCATACATGGTAACCGATTCTGA                   CAAAATGGAAGCAGTTCTTGAAAATCCATATATTTTAATTACGGATAAAAAGATTGCAAGCATTCAAGAA                   ATCCTACCAGTTCTTGAGCAAGTGGTTCAACAAGGTAAACCAATCCTAATCATCGCTGAAGATGTTTGAA                   GGGGAAGCTCAAGCAACATTAGTTGTTAATAAATTACGTGGTACATTCAATGCGGTAGCCGTT                   (SEQ ID NO:107)                 A. aneruino-     cpn60   ATCC12856   GCTACAGTTCTTGCTCAAGCGATGATTCGCGAAGGCTTGAAAAACGTAACAGCGGGTGCAAACCCGATGG         lyticus             TTATGCGCAAAGGTATCGAAAAGGCAGTTCGTGCAGCAGTAGAAGAACTGCATGCGATTTCTAAACCAAT                   CGAAGGTAAAGAATCTATCGCACAAGTAGCAGCTATTTCTGCTGCTGATGAGGAAATCGGCCAACTGATT                   GCTGAAGCTATGGAAAAAGTAGGAAAAGATGGCGTTATCACAGTAGAAGAATCCAAAGGCTTCACAACAG                   AACTTGATGTTGTAGAAGGTATGCAATTCGACCGCGGATACGCTTCTCCATACATGATCACGGATACTGA                   TAAGATGGAAGCAGTGCTTGATAATCCGTATATCTTGATTACGGATAAGAAAATCTCTAACATTCAGGAA                   ATCCTTCCTGTGTTAGAGAAAGTTGTACAACAAGGCAAGCCGCTTGTTATCATCGCTGAAGATGTAGAAG                   GCGAAGCACTGGCTACGCTTGTTGTAAATAAATTGCGTGGTACATTTACTGCGGTAGCAGTA                   (SEQ ID NO:108)                 A. xylanus     cpn60   ATCC   GCAACAGTTTTAGCACAAGCAATGATTAAGAAGGATTGAAAAACGTTGCTTCTGGACCAAACCCTGTCG               51415   GTGTTCGCCGTGGAATTGAAAAAGCTGTTGAAGTTGCAGTAGACGAGCTTAGAAAAATTTCACAAACAG                   TTGAAGATAAAGAATCAATCGCTCAAGTTGCAGCTATTTCAGCAAATGACGAAGAAGTAGGTCAATTAA                   TCGCTGAAGCAATGGAGCGCGTTGGTAAGATGGTGTAATTACTGTTGAAGAATCAAGAGGATTCAGCAC                   TGAACTTGAAGTAGTAGAAGGTATGCAATTTGACCGCGGATATACTTCACCATATATGGTATCTGACCA                   AGATAAGATGGAAGCAGTGCTTGAAGATCCATATATTTTAGTAACAGATAAGAAATTAACACATTCAAG                   ATGTATTACCAGTACTTGAGCAAGTTGTACAACAAAGCAAGCCACTATTAATTATTGCTGAAGATGTTG                   AAGGTGAAGCACTTGCAACATTGGTTGTAAACAAACTTCGTGGAACATTTAATGCAGTAGCTGTA                   (SEQ ID NO:109)                 V. pantothen-     cpn60   ATCC14576   GCAACTGTATTAGCTCAGTCCATGATTCGTGAAGGTCTTAAACGTAGCATCCGGTGCTAACCCTGTTGG         ticus (=B65             TGTTCGCCGCGGAATCGAAAAGGCTGTTGAAGTAGCAGTAAAAGAACTAAAAATATTTCCAAGTCAATC         B. panthothen-             GAAAGCAAGGAATCTATTGCTCAAGTAGCAGCAGTTTCTTCTGACGATGCAGAAGTTGGTAAGTTAATT         ticus )           TCTGAAGCAATGGAACGTGTTGGTAACGACGGAGTTATTACTATTGAAGAATCAAAAGGTTTCAACACA                   GAGCTAGAAGTAGTTGAAGGTATGCAATTTGACCGTGGATATGCTTCTCCATACATGGTAACAGACCAA                   GACAAAATGGAAGCAGTTTTGGAAAATCCGTACATCCTAATTACGGATAAGAAAATTGGTAACATTCAA                   GAAGTATTACCTATACTTGAACAAGTTGTACAGCAAGGAAAACCTTTATTGATGATTGCTGAGGATGTA                   GAAGGCGAAGCGCTTGCTACATTAGTAGTTAACAAATTGCGTGGAACATTCAATGCAGTAGCTGTA                   (SEQ ID NO:110)                 P. popillae     cpn60       GCTACGGTTCTGGCTCAAGCGATGATTCGCGAAGGCTTGAAGAACGTTACGGCTCGCGCGAATCCGATG                   GTCGTTCGCATCAAGGGATCGAGAAAGCAGTGAAANCCGCTGTTGAAGATCTGAAGAAAATTGCGAAGC                   CAATTGAAAACAAGCAAGCATCGCTCAAGTTGCTGCAATCTCTNCCGATGACGAAGAAGTCGGCACATT                   GATCGCAGAAGCAATGGAGAGAGTCGGCAATGACGGTGTAATTACGGTTGAGGAATCCAAAGGCTTCAA                   TACGGAGCTTGAAGTTGTAGAAGGGATGCNATTNGACCNTGGCTNTNTATCTCCGTACATGATCACGGA                   TACGGACAAGATGGAAGCTATCCTCGATACCCCATATATCTTGATCACAGATAAGAAGGTTTCCAACAT                   TCAAGAAATCCTTCCTGTTCTTGAGAAAGTCATTCAACAAGGCAAGCAGCTCCTGATCATCGCTGAGGA                   TGTAGAAGGCGAGCTCAAGCAACCTTGATCTTGAATAAGCTTGCGGACATTCACTTGCGTTGCCGTTA                   (SEQ ID NO:111)                 S. pyogenes     cpn60   ATCC19615   GCAACAGTTTTGACACAAGCCATTGTTCATGAAGGACTAAAAAATGTGACAGCAGGTGCTAATCCAATT                   GGTATCCGTCGAGGCATTGAAACAGCAACAGCAACAGCCGTTGAAGCCTTGAAAGCCATTGCTCAACCT                   GTATCTGGCAAGGAAGCTATTGCTCAGGTCGCTGCAGTATCATCACGCTCTGAAAAAGTTGGAGAGTAT                   ATCTCAGAAGCTATGGAGCGTGTGGGCAACGATGGTGTGATTACCATCGAAGAATCTCGAGGTATGGAA                   ACAGAACTTGAAGTG0TTGAAG0CATGCATTTGACCGTGGTTACCTGTCTCAATACATGGTCACAGACA                   ATGAAAAAATGGTTGCAGACCTTGAAAACCCATTTATCTTGATCACGGATAAAAAAGTGTCAAACATCC                   AAGACATTTTGCCACTACTTGAGGAAGTTCTTAAAACCAACCGTCCATTACTCATTATTGCAGATGATG                   TGGATGGTGAGCCCTTCCAACCCTTGTCTTGAACAAGATTCGTGGTACTTTCAATGTGGTTGCTGTC                 Escherichia-     cpn60       GCAACCGTACTGGCTCAGGCTATCATCACTGAAGGTCTGAAAGCTGTTGCTGCGGGCATGAACCCGATG         coli  K12           GACCTGAAACGTGGTATCGACAAAGCGGTTACCGCTGCAGTTGAAGAACTGAAAGCGCTGTCCGTACCA                   TGCTCTGACTCTAAAGCGATTGCTCAGGTTGGTACCATCTCCGCTAACTCCGACGAAACCGTAGGTAAA                   CTGATCGCTGAAGCGATGGACAAAGTCGGTAAAGAAGGCGTTATCACCGTTGAAGACGGTACCGGTCTG                   CAGGACGAACTGGACGTGGTTGAAGGTATGCAGTTCGACCGTGGCTACCTGTCTCCTTACTTCATCAAC                   AAGCCGGAAACTGGCGCAGTAGAACTGGAAAGCCCGTTCATCCTGCTGGCTGACAAGAAAATCTCCAAC                   ATCCGCGAAATGCTGCCGGTTCTGGAAGCTGTTGCCAAAGCAGGCAAACCGCTGCTGATCATCGCTGAA                   AGATGTAGAAGGCGAAGCGCTGGCAACTCTGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGC                   GGTT (SEQ ID NO:113)                 Brassica-     cpn60       TCTGTGGTTCTTGCACAAGGTTTTATTGCTGAGGGTGTCAAGGTGGTGCCTGCTGGTGCAAACCCTGTA         napus             TTGATCACTAGAGGCATTGAGAAGACAGCAAAGGCTTTGGTAGCCGAGCTCAAGAAAATGTCTAAGGAG         chloroplast             GTTGAAGACAGTGAGCTTGCAGATGTGGCAGCCGTTAGTGCCGGTAACAATGCAGAAATTGGAAGCATG         beta             ATTGCTGAAGCAATGAGCAGAGTGGGCAGGAAGGGTGTGGTGACACTTGAGGAGGGTAAAAGTGCAGAG                   AACGCTCTCTACGTGGTGGAAGGAATGCAATTTGATCGAGGTTATGTCTCCCCTTACTTTGTGACAGAC                   AGCGAGAAAATGTCAGTTGAGTTCGACAATTGCAAGTTGCTTCTTGTTGACAAGAAAATTACCAATGCA                   AGGGATCTTGTTGGTGTTCTGGAGGATGCAATTAGAGGAGGATACCCAATTTTAATAATTGCGGAAGAC                   ATTGAGCAGGAGGCTTTAGCGACCCTTGTTGTTAACAAGCTTAGAGGCACACTGAAGATTGCAGCTCTC                   (SEQ ID NO:114)                  
 
         [0089]     Within this simple system of 16S and cpn60 amplicons from a single species hybridized to amplicon probes of perfect match on the array, the dual backbone prototype was easily able to distinguish three of the four species tested in this assay.  H. halophilus  gave a strong signal only for its matching 16S and cpn60 probes ( FIG. 6 ).  A. xylanus  gave a strong signal for the 16S of  H. halophilus  in addition to its matching 16S probe ( FIG. 4 ). However, the only cpn60 signal came from the probe for  A. xylanus . There was no strong signal for its corresponding 16S probe, when  V. pantothenticus  was hybridized, due to irregularities in the printed DNA spot ( FIG. 7 ). However, two cpn60 probes gave signals— B. pantothenticus  and  V. pantothenticus . A closer look at these two amplicons revealed that the sequences were identical. Even with an identical sequence, the signal was significantly stronger for the  B. pantothenticus  than for the  V. pantothenticus , which had less DNA deposited on the array (determined by a deoxynucleotidyl terminal transferase assay).  
         [0090]     The above three hybridizations were all done with  Bacillus -like species that have been reclassified into new genera based on a significant difference from the core  Bacillus  species.  B. amyloliquefaciens  ( FIG. 5 ) gave several signals for the 16S probes. It appeared that the  B. amyloliquefaciens  probe was the strongest, but it was difficult to confirm due to spot irregularities (the mooning effect). When examining the cpn60 probes, signals were obtained from  B. subtilis  and  B. amyloliquefaciens . A closer look at the cpn60 for  B. subtilis  showed a 6% difference in sequence similarity, which is believed to be too close to discriminate using microarrays. In this case the dual backbone array was able to identify the sample as a  Bacillus , and even narrow it down to a pair of species, but it was not able to positively identify it as  B. amyloliquefaciens.    
         [0091]     From the results obtained above, it was concluded that the optimal hybridization temperature varied between the 16S and cpn60 amplicons, but a compromise at 55° C. at which both types of amplicons hybridized with adequate specificity was appropriate.  
         [0092]     By simultaneously assaying for virulence and antimicrobial resistance genes on the same microarray a significant reduction of effort and time were achieved.  
         [0093]     The oligonucleotide microarray of the present invention is a powerful tool for the detection of virulence and antimicrobial resistance genes in  E. Coli  strains.  
         [0094]     In accordance with the present invention, it is the first time according to the inventors that two different types of taxonomic sequences (16S and cpn60) have been used together and the results analyzed jointly to obtain corroboration that in some case it is not possible to have otherwise. It is also the first time that antibiotic resistance genes have been used with virulence genes in  E. coli  on the same array to obtain, in one experiment, information on the nature of the pathogen and how best to treat it. It is also the first time that many variants of the genes probes for virulence are being disclosed to pinpoint the precise type and, in some cases, the target species of the pathogen detected. Thus through a combination of probes, the inventors achieve a better and faster results than previously possible with DNA microarrays of the prior art.  
         [0095]     While the invention has been described in connection with specific embodiments thereof, it will be understood that it is capable of further modifications and this application is intended to cover any variations, uses, or adaptations of the invention following, in general, the principles of the invention and including such departures from the present disclosure as come within known or customary practice within the art to which the invention pertains and as may be applied to the essential features hereinbefore set forth, and as follows in the scope of the appended claims.