Abstract:
The invention is a cell culture medium that can include reduced or no serum and that enhances the performance of serum-free media for cell culture. The medium supports the growth of cells for both small scale and large scale propagation of cells. The invention also includes a method of cultivating cells using the cell culture medium of the present invention.

Description:
[0001]     This application claims priority to U.S. Ser. No. 60/535,580 filed Jan. 9, 2004, and U.S. Ser. No. 60/568,084 filed May 4, 2004, the contents of which are herein incorporated by reference. 
     
    
     FIELD OF THE INVENTION  
       [0002]     The invention is a cell culture medium that can include reduced or no serum and that enhances the performance of serum-free media for cell culture. The medium supports the growth of cells for both small scale and large scale propagation of cells. The invention also includes a method of cultivating cells using the cell culture medium of the present invention.  
       BACKGROUND OF THE INVENTION  
       [0003]     Biotechnology drugs are medicines, such as therapeutic proteins (monoclonal antibodies, blood proteins and enzymes) that are produced by living organisms to fight disease. Unlike other medicines, biotech drugs are generally not produced synthetically, but are usually produced through microbial fermentation in mammalian cell culture. They can be more difficult, time-consuming and expensive (at least $250 million in production facility costs alone) to produce than synthetic drugs.  
         [0004]     It is estimated that there are more than 370 new biotechnology medicines in the pipeline. Producing biotech drugs is a complicated and time-consuming process. Cells must be grown in large stainless-steel fermentation vats under strictly maintained and regulated conditions. In some cases the proteins are secreted by the cells; in other cases the cells must be broken open so the protein can be extracted and purified. Once the method is tested, devised and scaled up, the biotech medicines can be produced in large batches. This is done by growing host cells that have been transformed to contain the gene or antibody of interest in carefully controlled conditions in large stainless-steel tanks. The cells are kept alive and stimulated to produce the target proteins through precise culture conditions that include a balance of temperature (which can often vary by no more than one degree Celsius), oxygen, acidity (if pH levels change by even a small fraction, cells can easily die), media components and other variables. After careful culture in the appropriate media or serum (the duration varies depending on the protein produced and the nature of the organism), the proteins are isolated from the cultures, stringently tested at every step of purification, and formulated into pharmaceutically-active products. All of these procedures are in strict compliance with Food and Drug Administration (FDA) regulations. (htp://www.bio.org/pmp/factsheet1.asp, “A Brief Primer on Manufacturing Therapeutic Proteins”).  
         [0005]     There are many varied types of cell culture media that can be used to support cell viability, for example DMEM medium (H. J. Morton, In Vitro, 6, 89/1970), F12 medium (R. G. Ham, Proc. Natl. Acad. Sci. USA, 53, 288/1965) and RPMI 1640 medium (J. W. Goding, J. Immunol. Methods, 39, 285/1980; JAMA 199, 519/1957). Such media (often called “basal media”), however, are usually seriously deficient in the nutritional content required by most animal cells. Typically, serum must be added to the basal media to overcome these deficiencies. Generally, fetal bovine serum (FBS), horse serum or human serum is used in significant concentrations.  
         [0006]     While the use of FBS is desirable, and often necessary, for proper cell growth, it has several disadvantages. It is a relatively expensive material, and its use greatly increases the cost of cell culture. In addition, it is difficult to obtain serum with consistent growth characteristics. Further, the biochemical complexity of FBS can complicate the downstream processing of the proteins of interest, therefore raising the production costs.  
         [0007]     The revolution in cell culture techniques is prompting research on commercial scale processes. Research and development spending, along with increasing competition, new products, the international marketplace and a changing customer base (due to the use of serum free media) are contributing to the creation of a new kind of market in this field. Serum-free medium is an excellent alternative to standard serum-containing media for the cultivation of cells. It has several advantages, which include better definition of the composition, reduced contamination and lower cost.  
         [0008]     A serum-free medium having cultivation ability comparable to that of the conventional serum-containing medium has long been sought. There is a continuing need in the art for cell culture media that are simple to prepare, economical, and that provide all of the necessary nutrients and growth factors, at suitable concentrations, to optimize the growth of the cells.  
         [0009]     One strategy to develop serum-free media has been to supplement the basal media with appropriate nutrients to avoid the addition of FBS, without sacrificing cell growth and/or protein production. Examples of such components include bovine serum albumin (BSA) or human serum albumin (HSA); certain growth factors derived from natural (animal) or recombinant sources, including epidermal growth factor (EGF) or fibroblast growth factor (FGF); lipids such as fatty acids, sterols and phospholipids; lipid derivatives and complexes such as phosphoethanolamine, ethanolamine and lipoproteins; protein and steroid hormones such as insulin, hydrocortisone and progesterone; nucleotide precursors; and certain trace elements (reviewed by Waymouth, C., in: Cell Culture Methods for Molecular and Cell Biology, Vol. 1: Methods for Preparation of Media, Supplements, and Substrata for Serum-Free Animal Cell Culture, Barnes, D. W., et al., eds., New York: Alan R. Liss, Inc., pp. 23-68 (1984), and by Gospodarowicz, D., Id., at pp 69-86 (1984)).  
         [0010]     It is known that cholesterol and cholesterol-containing fractions obtained from bovine serum are useful to promote the growth of various organisms. J. Bacteriol., Vol. 135, pp. 818-827 (1978) describes the use of a cholesterol-containing bovine serum fraction in the growth of Mycoplasma pneumoniae and Mycoplasma arthritidis. J. Gen. Microbiology, Vol. 116, pp. 539-543 (1980) describes the use of USP cholesterol in the growth of Treponema hyodysenteriae. In Vitro, Vol. 17, No. 5, pp. 519-530 (1981) discloses that mixtures of high density lipoproteins and transferrin can be used to grow certain mammalian cells in the absence of serum.  
         [0011]     U.S. Pat. No. 4,290,774 describes the production of a specific cholesterol-rich fraction from mammalian plasma or serum by a process that involves the step of treatment with an alkaline carbonate and an alkaline earth salt. Zeit. Klin. Chem. 6(3), pp. 186-190 (1968) describes the removal of certain lipoproteins from human serum by use of colloidal silicic acid.  
         [0012]     U.S. Pat. No. 4,762,792 and European Patent No. EP0201800 disclose a process for isolating a cholesterol-rich fraction from mammalian blood plasma or serum using a silica adsorbant followed by several alkaline steps, which is useful as a growth medium ingredient, especially in cell culture.  
         [0013]     U.S. Pat. No. 5,409,840 describes an improved process for the recovery of cholesterol rich fractions from mammalian serum or plasma. The process involves adsorbing the fraction on precipitated silica gel agglomerates which are then separated from the serum or plasma whereupon the adsorbed cholesterol rich fraction is eluted from the silica and recovered.  
         [0014]     EX-CYTE® is a concentrated aqueous mixture of cholesterol, lipoproteins and fatty acids that is manufactured by Serologicals, Inc. using the process described in U.S. Pat. No. 4,762,792. EX-CYTE® is typically made from bovine serum.  
         [0015]     In 1989, Hewlett et al. (Miles Science Journal 1989,11: 9-14) described the effects of the addition of EX-CYTE® to serum-free or low serum containing culture media on the growth of several cell types, including L929 cells, CHO-K1 cells, BHK-21 cells, AHT-107 hybridoma cells, mouse myeloma cells and monkey-fibroblast cells. The cells were grown in media containing several of the following components (the components varied depending on cell type): insulin, transferrin, selenite, bovine EX-CYTE®, human EX-CYTE®, freeze-dried EX-CYTE®, 1% FBS, selenite, human serum albumin (HAS) and/or trace elements.  
         [0016]     In 1991, Guy Hewlett (Prod. Biol. Anim. Cells Cult. (1991) ESACT 10 Meet., 67-69) further described the effect of the addition of EX-CYTE®, to serum-free or low serum containing culture media on the growth of several cell types, including A431 human keratinocytes, L929 mouse fibroblast cells, 3T3 cells, X63 myeloma cells, AHT hybridoma cells and HeLa cells. The cells were grown in media containing 50/50 mix of Dulbecco&#39;s modification of Eagle&#39;s medium (DMEM) and Ham&#39;s nutrient solution F12 (F12) and several of the following additional components (the components varied depending on cell type): bovine insulin (10 mg/L), transferrin (10 mg/L), EX-CYTE®, lipoprotein/lipid (30 ug cholesterol/mL), albumin (200 mg/L), selenium (100 nmol/L) and/or 0.5% Fetal Calf Serum.  
         [0017]     Savonniere et al. (Journal of Biotechnology 48 (1996) 161-173) described the effect of lipid supplementation of culture media on cell growth, antibody production, membrane structure and dynamics in two hybridoma cell lines (B9 cells (fusion of SP2/OAg14 cells with mouse Balb/C spleen cells) and A49 cells (fusion of SP2/O myeloma cells with mouse Balb/B lymphocytes)). B9 cells were grown in RPMI 1640 medium (Seromed. Strasbourg, France) supplemented with 100 UI/ml penicillin, 10 μg/ml streptomycin, 5% {v/v) fetal calf serum, 50 μM 2-mercaptoethanol, 100 UI/ml of recombinant human IL-6, and Ex-Cyte. A49 cells were grown in RPMI 1640 medium supplemented with antibiotics, 0.5% (v/v) FCS, 2% Ultroser HY and EX-CYTE®. They reported that the response to the two cell lines to EX-CYTE® was different, the addition of EX-CYTE® was without effect on the B9 cells, while A49 cells showed an increased growth rate.  
         [0018]     PCT Publication No. WO 90/07007 filed by the United States of America discloses a serum free media for culturing animal epithelial cells, including human epithelial cells. The patent discloses a media with the following components: L-glutamine, 2 mM, Insulin, 10 ug/ml, Hydrocortisone, 0.2 uM, epidermal growth factor, 5.0 ng/ml, transferrin, 10 ug/ml, phosphoethanolamine, 0.5 uM, cholera toxin, 25 ng/ml, triiodothyronine, 10 nM, retinoic acid, 10 nM, ornithine, 2 mM, CaCl 2 , 0.4 mM, Glucose, 2.0 mg/ml, bovine pituitary extract, 7.5 ug/ml, EX-CYTE® V, 312 ug/ml, FeSO 4 ≅7H 2 O, 2.7 uM, ZnSO 4 ≅7H 2 O, 0.5 uM, Na 2 SeO 3 , 3.0×10 −8  M, MnCl 2 ≅4H 2 O, 1.0 nM, Na 2 SiO 3 ≅9H 2 O, 5.0×10 −7  M, (NH 4 ) 6  Mo 7 O 24 ≅4H 2 O, 1.0 nM, NH 4 VO 3 , 5.0 nM, NiSO 4 ≅6H 2 O, 0.5 nM, SnCl 2 ≅2H 2 O, 0.5 nM, and Gentamicin, 50 ug/ml.  
         [0019]     U.S. Pat. No. 6,733,746 to Daley et al. and U.S. Publication No. 2004/0072349 filed by Daley et al. disclose a hematopoetic cell culture nutrient supplement. The supplement disclosed contains one or more antioxidants, one or more albumins or albumin substitutes, one or more lipid agents, one or more insulins or insulin substitutes, one or more transferrins or transferrin substitutes, one or more trace elements, and one or more glucocorticoids. The patent application specifically discloses formulations for culturing hematopoetic stem cells that contain, for example N-acetyl-L-cysteine, human serum albumin, Human EX-CYTE®, ethanolamine HCl, zinc insulin, human iron saturated transferrin, a Se 4 +salt, hydrocortisone, D,L-tocopherol acetate, 2-mercaptoethanol and/or glutamine.  
         [0020]     U.S. Pat. No. 5,932,703 to ICOS Corporation describes purified and isolated nucleotide sequences encoding a human macrophage-derived chemokine (MDC) and methods for the recombinant production of the same. Transfected CHO cells were used to express MDC. The media used to culture the CHO cells contained P5 medium (which consists of various components including glutamine) containing 0.2% to 1.0% FBS, 3 g/l sodium bicarbonate, 2 ug/l sodium selenite, 1% soy bean hydrolysate, ferrous sulfate/EDTA solution, 1.45 ml/L EX-CYTE VLE solution, 10 ug/ml recombinant insulin, 0.1% pluronic F-68, 30 ug/ml glycine, 50 uM ethanolamine and 1 mM sodium pyruvate.  
         [0021]     Gorfien et al. ( Biotechnol. Prog.  2000, 16, 682-687) describe the growth of NSO hybridoma cells in a protein free, chemically defined media. The media contains CD Hybridoma Medium and lipoprotein supplements, including EX-CYTE VLE (Bayer, catalog number 81-129) at 1:300, 1:500, and 1:1000 dilutions. This reference teaches that Ex-Cyte did not support the long term growth of NSO cells.  
         [0022]     U.S. Patent Publication No. 2003/0166146 to Lee et al. describes a myeloma line useful for manufacturing recombinant proteins in chemically defined media. The chemically defined media used to culture the myeloma cell line (C463A myeloma cell line, a spontaneous mutant cloned from a Sp2/0-Ag14 cell bank) contained IMDM, Primatone, Albumin, and Ex-Cyte.  
         [0023]     U.S. Pat. No. 5,240,848 to Monsanto Company describes a cDNA sequence for human vascular permeability factor and methods to recombinantly produce the same. U-937 cells (a human cell line established from a diffuse histiocytic lymphoma, ATCC CRL 1593) were used to produce the vascular permeability factor protein. The cells were cultured in media that contained the following components: RPMI 1640, DME (high glucose), Ham&#39;s F12 in a 1:1:1 ratio, HEPES (25 mM, pH 7.10-7.15) glutathione (1 mM), ethanolamine (20 uM), selenium (30 nM) or 5200 ug/ml, NaHCO 3  (2 mM), CuSO 4  (5 nM), NH4 VOs (5 nM), ZnSO 4  (0.5 uM), MnSO 4  (0.5 nM), FeSO 4  (4 uM), bovine serum albumin, Miles “Pentex” (100 ug/ml), iron rich transferrin, Miles (5 ug/ml), bovine insulin (10 ug/ml), F-68 Pluracol (0.05% w/v) and 0.1% Ex-Cyte.  
         [0024]     In 2002, Serologicals, Inc. published a Technical Bullitin disclosing increased protein yield in an antihuman IgGG monoclonal antibody-producing cell line. The publication disclosed the use of Ex-Cyte (30 ug cholesterol), human serum albumin (10 mg/ml), human APO-transferrin (2.5 ug/ml), insulin (5 ug.ml) and sodium selenite (10 −7 M).  
         [0025]     It is an object of this invention to provide culture media that supports the growth of various cell types.  
         [0026]     Another object of this invention is to provide culture media that are serum-reduced or serum-free to support the growth of various cell types.  
         [0027]     A further object of the present invention is to provide cell culture media for use in either small-scale culture or large-scale commercial production of cells.  
         [0028]     A still further object of this invention is to provide a cell culture medium that increases the yield of biological materials, for example, peptides, produced by the cells cultured in such media.  
         [0029]     Another object of this invention is to provide a method of culturing cells in a suitable medium to allow cell growth.  
         [0030]     A further object of this invention is to provide a method of culturing cells in a suitable medium for the production of biological materials of interest.  
         [0031]     A still further object of the present invention is to provide methods to make cell culture media.  
       SUMMARY OF THE INVENTION  
       [0032]     The invention provides novel cell culture media compositions that include purified lipoprotein material that reduces or eliminates the use of serum or enhance the performance of serum-free media for cell culture. The invention also includes methods of culturing cells using the cell culture media compositions. The compositions and/or methods are useful in the culture of a variety of cell types, including, for example, hybridoma cells and/or cancer cells.  
         [0033]     In a first embodiment, the cell culture composition can include (i) basal media; (ii) purified lipoprotein material; and/or (iii) a reduced concentration of serum, such as fetal bovine serum (FBS), relative to the use of serum alone to supplement basal media. Normally, basal media is supplemented with approximately 10% (alternatively by weight or volume) or more serum, such as FBS. In this embodiment, basal media can be supplemented with less than 10% serum, such as FBS, and/or more particularly, approximately 9.5, 9, 8.5, 8, 7.5, 7, 6.5, 6, 5.5, 5, 4.5, 4, 3.5, 3, 2.5, 2, 1.5, 1, 0.5 or 0.25% serum, such as FBS, in combination with a purified lipoprotein material. In one embodiment, between 0.1 and 5% purified lipoprotein material can be used in the composition. In another embodiment, up to approximately 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.75, 0.8, 0.9, 1.0, 1.5 or 2% purified lipoprotein material is used. In another embodiment, approximately 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.75, 0.8, 0.9, 1.0, 1.5 or 2% purified lipoprotein material can be used.  
         [0034]     In embodiments of the present invention, the purified lipoprotein material can be derived from serum or plasma obtained from a mammal. In one embodiment, the purified lipoprotein material can be a cholesterol-rich fraction, optionally in association with low density lipoprotein (LDL) and/ or high density lipoprotein (HDL). In another embodiment, the purified lipoprotein material can be derived from bovine, horse, sheep, pig or human serum or plasma. In a further embodiment, the purified lipoprotein material can contain cholesterol, such as approximately 5-15, more particularly, 9, 10 or 11, grams per liter of cholesterol as determined, for example, by enzymatic assay; protein, such as approximately 10-20, more particularly, 13, 14, 15, 16, 17 or 18, grams per liter of protein, and/ or approximately 0-10, particularly, 0-6, EU endotoxin per milligram of cholesterol, such as determined, for example, by limulus amebocyte lysate. In another embodiment, the pH of the purified lipoprotein material can be between approximately 7 and 8, more particularly, 7.0-8.4. In other embodiments, the purified lipoprotein material does not contain detectable levels of contaminants or undesired materials, for example, immunoglobulin G (IgG), microbes, mycoplasm, and/or viral agents.  
         [0035]     In further embodiments, the purified lipoprotein material can be produced by contacting the plasma or serum or derivative thereof with an adsorbant, such as silica. In a specific embodiment, the purified lipoprotein material produced according to the following process: (a) contacting a liquid cholesterol-containing plasma or serum or fraction thereof with a silica adsorbent to adsorb the cholesterol-rich fraction; (b) separating the adsorbed cholesterol-rich fraction from the remaining liquid plasma or serum; (c) freezing and/or thawing the adsorbed cholesterol-rich fraction; (d) eluting the adsorbed cholesterol-rich fraction at a pH from 9.0 to 11.5; (e) either before or after step (f) and/or prior to step (g) adjusting the pH of the cholesterol-rich solution to a value in the range from 11.0 to 13.0; (f) concentrating the cholesterol-rich solution by ultrafiltration; (g) dialyzing the concentrated cholesterol-rich solution sequentially against sodium carbonate and/or water; (h) further concentrating the dialyzed cholesterol-rich solution by ultrafiltration; (i) adjusting the pH of the concentrated cholesterol-rich solution to a value in the range from 7.0 to 11.0; (j) heating the concentrated cholesterol-rich solution at 50° to 100° C. for 30 minutes to 24 hours; (k) recovering therefrom a purified cholesterol-rich fraction, such as described in detail in U.S. Pat. No. 4,762,792, including but not limited to the product EX-CYTE®.  
         [0036]     In another embodiment, the composition can include (i) basal media; (ii) purified lipoprotein material; (iii) insulin; (iv) albumin; (v) sodium selenite; and/or (vi) transferrin. In one embodiment, the composition can include approximately 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5 or 9 mg/ml albumin, such as bovine serum albumin (BSA); at least 3.0, 3.5, 4.0, 4.5, 5.0, 5.5, 6.0, 7.0, 10, 15 or 20 ug/ml transferrin; approximately 5.5, 6.0, 6.5, 7.0, 7.5, 8.0, 8.5, 9.0, 9.5, 10, 10.5, 11, 11.5, 12, 15 or 20 ug/ml insulin; approximately 1, 2, 3, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 6.7, 7.0, 7.5, 8.0, 9.0, 10, 15 or 20 ug/L sodium selenite; and/or 0.2, 0.3., 0.4, 0.5, 0.6, 0.7, 0.75, 0.8, 0.9, 1.0, 1.5 or 2% purified lipoprotein material in basal media. In one specific embodiment, the cell culture media can include approximately 4 mg/ml BSA; approximately 5.5 ug/ml transferrin; approximately 10 ug/ml insulin; approximately 6.7, ug/L sodium selenite; and/or approximately 0.75% purified lipoprotein material in basal media.  
         [0037]     In another embodiment, the composition can include (i) basal media; (ii) purified lipoprotein material (iii) glutamine; (iv) albumin; (v) insulin; (vi) transferrin; and/or (vii) ethanolamine. In one embodiment, the composition can include approximately 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 mM glutamine; approximately 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 2, 3, 4, 5, 6, 7, or 8% albumin, such as bovine serum albumin (BSA); approximately 1, 2, 3, 4, 5, 6, 7, 8, 9, 9.5, 10, 10.5, 11, 11.5, 12, 13, 14, 15, 16, 17, 18, 19, 20 mg/L insulin; approximately 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 0.95, 1, 1.5, 2, 2.5, 3, 4, 5, 6, or 7 mg/L transferrin; approximately 1, 2, 3, 4, 5, 6, 7, 8, 8.5, 9, 9.5, 10, 10.5, 11, 11.5, 12, 13, 14, 15, 16, 17, 18, 19 or 20 μM ethanolamine and/or approximately 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.5, 2, 2.5, 3, 3.5, 4, or 5.0 % purified lipoprotein material in basal media. In one specific embodiment, the invention can include approximately 4 mM glutamine; approximately 0.5% BSA; approximately 10 mg/L insulin; approximately 1 mg/L transferrin; and/or approximately 10 molar ethanolamine, and/or approximately 2% purified lipoprotein material in basal media.  
         [0038]     In another embodiment, the composition can include (i) basal media; (ii) purified lipoprotein material; (iii) glutamine; (iv) albumin; (v) insulin; and/or (vi) transferrin. In one embodiment, the composition can include approximately 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 mM glutamine; approximately 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.5, 2, 2.5, 3, 3.5, 3.5 to 5, 5 to 10, 10 to 20% albumin, such as bovine serum albumin (BSA); approximately 1, 2, 3, 4, 5, 6, 7, 8, 9, 9.5, 10, 10.5, 11, 11.5, 12, 13, 14, 15, 16, 17, 18, 19, or 20 mg/L insulin; and/ or approximately 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 0.95, 1, 1.5, 2, 2.5, 3, 4, 5, 6, or 7 mg/L transferrin. In one specific embodiment, the composition can include approximately 4 mM glutamine, approximately 0.1% purified lipoprotein material, approximately 1% BSA, approximately 10 mg/L insulin, and/or approximately 1 mg/L transferrin in basal media.  
         [0039]     In another embodiment, the composition can include (i) basal media; (ii) purified lipoprotein material; (iii) glutamine; (iv) albumin; (v) insulin; and/or (vi) transferrin; and/or (vii) peptone. In one embodiment, the composition can include approximately 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 mM glutamine; approximately 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.5, 2, 2.5, 3, 3.5, 3.5 to 5, 5 to 10, 10 to 20% albumin, such as bovine serum albumin (BSA); approximately 1, 2, 3, 4, 5, 6, 7, 8, 9, 9.5, 10, 10.5, 11, 11.5, 12, 13, 14, 15, 16, 17, 18, 19, or 20 mg/L insulin; approximately 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 0.95, 1, 1.5, 2, 2.5, 3, 4, 5, 6, or 7 mg/L transferrin; and/or approximately 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 1, 2 or 3% peptone. In one specific embodiment, the composition can include approximately 4 mM glutamine, approximately 0.1% purified lipoprotein material, approximately 1% BSA, approximately 10 mg/L insulin, approximately 1 mg/L transferrin, and/or approximately 0.1% peptone in basal media.  
         [0040]     In embodiments of the present invention, the peptone or peptone mixture is a protein hydrolysate, which is obtained from hydrolyzed animal or plant protein. The peptones can be derived from animal by-products from slaughter houses, purified gelatin, or plant material. The protein from the animal or plant sources can be hydrolyzed using acid, heat or various enzyme preparations. Peptone mixtures that can be used include spy peptone, “Primatone RL” and/or “Primatone HS”, both of which are commercially available (Sheffield, England/or; Quest International (IPL:5X59051), PRIMATONE® RL).  
         [0041]     In another embodiment, the composition can include (i) basal media; (ii) purified lipoprotein material; (iii) glutamine; (iv) albumin; (v) insulin; (vi) transferrin; and/or (vii) fetuin (such as Pedersen). In one embodiment, the composition can include approximately 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 mM glutamine; approximately 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.5, 2, 2.5, 3, 3.5, 3.5 to 5, 5 to 10, 10 to 20% albumin, such as bovine serum albumin (BSA); approximately 1, 2, 3, 4, 5, 6, 7, 8, 9, 9.5, 10, 10.5, 11, 11.5, 12, 13, 14, 15, 16, 17, 18, 19, or 20 mg/L insulin; approximately 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 0.95, 1, 1.5, 2, 2.5, 3, 4, 5, 6, or 7 mg/L transferrin; and/or approximately 2, 3, 4, 5, 6, 7, 8, 9, 10, 10.5, 11, 11.5, 12, 12.5, 13, 13.5, 14, 14.5, 15, 16, 17, 18, 19, 20 μg/ml of fetuin. In one specific embodiment, the composition of the present invention can include approximately 4mM glutamine, approximately 0.1% purified lipoprotein material, approximately 1% BSA, approximately 10 mg/L insulin, approximately 1 mg/L transferrin, and/or approximately 12.5 μg/ml fetuin (such as Pedersens) in basal media.  
         [0042]     In another embodiment, the composition can include (i) basal media; (ii) purified lipoprotein material; (iii) glutamine; (iv) albumin; (v) insulin; (vi) transferrin; and/or (vii) vitamin E. In one embodiment, the composition can include approximately 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 mM glutamine; approximately 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.5, 2, 2.5, 3, 3.5, 3.5 to 5, 5 to 10, 10 to 20% albumin, such as bovine serum albumin (BSA); approximately 1, 2, 3, 4, 5, 6, 7, 8, 9, 9.5, 10, 10.5, 11, 11.5, 12, 13, 14, 15, 16, 17, 18, 19, or 20 mg/L insulin; approximately 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 0.95, 1, 1.5, 2, 2.5, 3, 4, 5, 6, or 7 mg/L transferrin; and/or approximately 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 micromolar vitamin E. In one specific embodiment, the composition of the present invention can include approximately 4 mM glutamine, approximately 0.1% purified lipoprotein material, approximately 1% BSA, approximately 10 mg/L insulin, approximately 1 mg/L transferrin, and/or approximately 5 μM vitamin E in basal media.  
         [0043]     In a preferred embodiment of the invention, the composition can include approximately 4 mM glutamine, approximately 0.1% purified lipoprotein material, approximately 1% BSA, approximately 10 mg/L insulin, approximately 1 mg/L transferrin, approximately 0.1% peptone, approximately 12.5 μg/mL fetuin (such as Pederson), and/or approximately 5 μM vitamin E.  
         [0044]     In a further embodiment, the composition can include (i) serum free media and/or (ii) purified lipoprotein material. In one embodiment, the serum free media is one of the media listed in Table 1. In another embodiment, the serum free media is either Hybridoma Media, animal component free or Ex-Cell (JRH Biosceinces, Inc.). In another embodiment, approximately 0.2, 0.3., 0.4, 0.5, 0.6, 0.7, 0.75, 0.8, 0.9, 1.0, 1.5 or 2% purified lipoprotein material can be used.  
         [0045]     In an additional embodiment, the composition can include (i) serum free media and/or (ii) purified lipoprotein material; and/or (iii) albumin. In one embodiment, the serum free media is one of the media listed in Table 1. In another embodiment, the serum free media is either Hybridoma Media, animal component free or Ex-Cell (JRH Biosceinces, Inc.). In a further embodiment, the composition can include approximately 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 2, 3, 4, 5, or 10% albumin, such as bovine serum albumin (BSA). In another embodiment, approximately 0.2, 0.3., 0.4, 0.5, 0.6, 0.7, 0.75, 0.8, 0.9, 1.0, 1.5 or 2% purified lipoprotein material is used. In a specific embodiment, the composition can include ExCell, 0.75% purified lipoprotein material ® and/or 0.5% BSA. In another specific embodiment, the composition can include Hybridoma Medium, Animal Component-free, 0.5% purified lipoprotein material and/or 0.2% BSA.  
         [0046]     In another aspect of the present invention compositions are provided that are useful as a cell culture medium that serves to increase the yield of biological products, such as proteins, produced by the cells cultured in the media. In one embodiment, compositions can increase the yield of biological products at least 25%, 30%, 50%, 100%, 200% or 300%. In another embodiment, the biological products produced can be a peptide, such as a therapeutic or diagnostic peptide, polypeptide, protein, monoclonal antibody, immunoglobulin, cytokine (such as interferon), integrin, antigen, growth factor, cell cycle protein, hormone, neurotransmitter, receptor, fusion peptide, blood protein and/ or chimeric protein.  
         [0047]     In a further aspect of the present invention, compositions are provided that are useful as a cell culture medium for a variety of cells. In one embodiment, the cell culture media of the present invention can be used for adherent cell culture. In another embodiment, the cell culture media described herein can be used for suspension cell culture. In other embodiments, the cell culture media described herein can be used as culture media for hybridoma cells, monoclonal antibody producing cells, virus-producing cells, transfected cells, cancer cells and/or recombinant peptide producing cells. In one embodiment, the compositions can be used to culture eukaryotic cells, such as plant and/or animal cells. The cells can be mammalian cells, fish cells, insect cells, amphibian cells or avian cells. Other types of cells can be selected from the group consisting of MK2.7 cells (ATCC Catalogue No. CRL1909, an anti-murine-VCAM IgG1 expressing hybridoma cell), HEK 293 cells, PER-C6 cells, CHO cells, COS cells, 5L8 hybridoma cells, Daudi cells, EL4 cells, HeLa cells, HL-60 cells, K562 cells, Jurkat cells, THP-1 cells, Sp2/0 cells; and/or the hybridoma cells listed in Table 2 or any other cell type disclosed herein or known to one skilled in the art.  
         [0048]     Basal media can include, but are not limited to Dulbecco&#39;s Modified Eagle&#39;s Medium (DMEM), Minimal Essential Medium (MEM), Basal Medium Eagle (BME), RPMI 1640, F-10, F-12, alpha. Minimal Essential Medium (.alpha.MEM), Glasgow&#39;s Minimal Essential Medium (G-MEM), and/or Iscove&#39;s Modified Dulbecco&#39;s Medium.  
         [0049]     The present invention also provides a method of cultivating eukaryotic cells including contacting the cells with the compositions that are useful as cell culture medium of the present invention and/or maintaining the cells under conditions suitable to support cultivation of the cells in culture. In a particular embodiment, the cells are cancer cells or hybridoma cells. In other embodiments, methods of cultivating tissue explants are cultures are provided including contacting the tissues with the cell culture media compositions described herein.  
         [0050]     In one embodiment, the method includes contacting hybridoma cells with a composition including: (i) basal media; (ii) purified lipoprotein material isolated as; (iii) glutamine; (iv) albumin; (v) insulin; (vi) transferrin; and/or (vii) ethanolamine, and/or maintaining the hybridoma cells under conditions suitable to support cultivation of the hybridoma cells in culture.  
         [0051]     In a specific embodiment, the method includes contacting hybridoma cells with a composition including (i) basal media; (ii) approximately 2% purified lipoprotein material; (iii) approximately 4 mM glutamine; (iv) approximately 0.5% BSA; (v) approximately 10 mg/L insulin; (vi) approximately 1 mg/L transferrin; (vii) approximately 10 μM ethanolamine.  
         [0052]     In another embodiment, the present invention is a method of cultivating cancer cells by contacting the cells with compositions that are useful as cell culture medium of the present invention and/or maintaining the cancer cells under conditions suitable to support cultivation of the cancer cells in culture. In a specific embodiment, the method includes contacting cancer cells with a composition including (i) basal media; (ii) purified lipoprotein material; (iii) glutamine; (iv) albumin; (v) insulin; and/or (vi) transferrin.  
         [0053]     In a specific embodiment, the method includes contacting cancer cells with a composition including (i) basal media; (ii) purified lipoprotein material; (iii) glutamine; (iv) albumin; (v) insulin; (vi) transferrin; and/or (vii) peptone.  
         [0054]     In a further embodiment, the method includes contacting cancer cells with a composition including (i) basal media; (ii) purified lipoprotein material; (iii) glutamine; (iv) albumin; (v) insulin; (vi) transferrin; and/or (vii) fetuin. In a specific embodiment, fetuin protein can be Pedersen&#39;s fetuin.  
         [0055]     In another embodiment, the method involves contacting cancer cells with a composition including (i) basal media; (ii) purified lipoprotein material; (iii) glutamine; (iv) albumin; (v) insulin; (vi) transferrin; and/or (vii) vitamin E.  
         [0056]     In a specific embodiment, the method of the present invention involves contacting cancer cells with a composition including (i) basal media; (ii) purified lipoprotein material; (iii) glutamine; (iv) approximately 1% BSA; (v) insulin; (vi) transferrin; (vii) peptone; and/or (viii) fetuin (such as Pedersens).  
         [0057]     In yet another embodiment, the method of the present invention involves contacting cancer cells with a composition including (i) basal media; (ii) purified lipoprotein material; (iii) glutamine; (iv) approximately 1% BSA; (v) insulin; (vi) transferrin; (vii) fetuin; and/or (viii) vitamin E.  
         [0058]     In a specific embodiment, the method of the present invention involves contacting cancer cells with a composition including (i) basal media; (ii) purified lipoprotein material; (iii) glutamine; (iv) approximately 1% BSA; (v) insulin; (vi) transferrin; (vii) peptone; (viii) fetuin (such as Pedersens); and/or (ix) vitamin E.  
         [0059]     In a particular embodiment, the method of the present invention involves contacting cancer cells with a composition including (i) basal media; (ii) approximately 0.1% purified lipoprotein material; (iii) approximately 4 mM glutamine; (iv) approximately 1% BSA; (v) approximately 10 mg/L insulin; (vi) approximately 1 mg/L transferrin; (vii) approximately 0.1% peptone; (viii) approximately 12.5 μg/ml fetuin (such as Pedersens); and/or (ix) approximately 5 μM vitamin E.  
         [0060]     In other embodiments of the present invention, methods are provided for the cultivation of cells wherein the cells can be incubated in a serum-containing media, such as about 0.5, 1, 2, 3, 4, 5, or 10% serum, such as FBS, followed by transfer of the cells into a serum-rescued or serum-free media of the present invention. In one embodiment, the cells can be grown to confluence and/ or maintained in serum-containing media and then transferred to the serum-free or serum-reduced media of the present invention. In one embodiment, the cells can be transferred to the media described herein prior to the production of biological materials from the cells. In an alternative embodiment, the cells can be grown only in serum-free or serum-reduced media of the present invention.  
         [0061]     The present invention also provides a kit for the cultivation of cells in vitro, the kit comprising the compositions of the present invention. In another embodiment, the kit can contain compositions of the present invention in combination with specific cell lines. 
     
    
     BRIEF DESCRIPTION OF THE FIGURES  
       [0062]      FIG. 1  demonstrates the cell growth comparisons of EX-CYTE®+2% FBS vs. 10% FBS vs. 2% FBS in DME/F12.  
         [0063]      FIG. 2  illustrates the IgG1 antibody production comparisons of EX-CYTE®+2% FBS vs. 10% FBS vs. 2% FBS in DME/F12.  
         [0064]      FIG. 3  demonstrates the cell growth comparisons of EX-CYTE®+BSA+Insulin+Transferrin+Sodium selenite (ITS) vs. 10% FBS in DMEM.  
         [0065]      FIG. 4  shows the IgG1 antibody production comparisons of EX-CYTE®+BSA+Insulin+Transferrin+Sodium selenite (ITS) vs. 10% FBS in DMEM.  
         [0066]      FIG. 5  illustrates cell growth comparisons of EX-CELL™ 620 vs. EX-CELL™ 620+EX-CYTE® and BSA.  
         [0067]      FIG. 6  demonstrates the IgG1 antibody production comparisons of EX-CELL™ 620 vs. EX-CELL™ 620+EX-CYTE® and BSA.  
         [0068]      FIG. 7  shows the cell growth comparisons of Hybridoma Medium, Animal Component-free vs. Hybridoma Medium, Animal Component-free+EX-CYTE® and BSA.  
         [0069]      FIG. 8  illustrates the IgG1 antibody production comparison of Hybridoma Medium, Animal Component-free vs. Hybridoma Medium, Animal Component-free EX-CYTE® and BSA.  
         [0070]      FIG. 9  depicts the results of the range find experiments to identify optimal concentrations of BSa and Ex-Cyte to enhance the growth of K562 cells.  
         [0071]      FIG. 10  depicts the results of the refined range find experiments to identify optimal concentrations of BSA and Ex-Cyte to enhance the growth of K562 cells.  
         [0072]      FIG. 11  demonstrated the results of a comparison study of the effects of Soy Peptone substituted for Primatone RL in the XCF-2 formulation on the growth of K562 cells.  
         [0073]      FIG. 12  demonstrates the performance of the XCF-2 media compared with the benchmark of 10% Fetal Bovine Serum on cell growth of K562 cells.  
         [0074]      FIG. 13  shows the levels of expression of the CD32 marker in K562 cells grown in different medias. Cells were grown in either 0.5% FBS or 10% FBS. In column 1, the cells were grown adapted to 0.5% FBS and maintained in that level for the experiment. In column 2 and 3, cells were grown in 10% FBS and then subsequently cultured in 10% FBS (column 2) or XCF2 (column 3) for the experiment. In column 4 and 5, cells were adapted to growth in 0.5% FBS and then subsequently cultured in 10% FBS (column 4) or XCF2 (column 5) for the experiment. 
     
    
     DETAILED DESCRIPTION  
       [0075]     Cultured cells are widely used in the biopharmaceutical industry for the production of biopharmaceuticals, such as vaccines, proteins, peptides and monoclonal antibodies. As scientists and cell culturists continue to strive maximize product yield and minimize costs, care selection of media and supplements is critical. The invention is a composition that is useful as a cell culture media that can include reduced or no serum or enhances the performance of serum-free media for cell culture.  
         [0076]     The invention provides novel cell culture media compositions that include purified lipoprotein material to reduce or eliminate the use of serum or enhance the performance of serum-free media for cell culture. The invention also includes methods of culturing cells using the cell culture media compositions. The compositions and/or methods are useful in the culture of a variety of cell types, including, for example, hybridoma cells and/or cancer cells.  
         [0077]     In a first embodiment, the cell culture composition can include (i) basal media; (ii) purified lipoprotein material; and/or (iii) a reduced concentration of serum, such as fetal bovine serum (FBS), relative to the use of serum alone to supplement basal media. In another embodiment, the composition can include (i) basal media; (ii) purified lipoprotein material; (iii) insulin; (iv) albumin; (v) sodium selenite; and/or (vi) transferrin. In a further embodiment, the composition can include (i) basal media; (ii) purified lipoprotein material (iii) glutamine; (iv) albumin; (v) insulin; (vi) transferrin; and/or (vii) ethanolamine. In another embodiment, the composition can include (i) basal media; (ii) purified lipoprotein material; (iii) glutamine; (iv) albumin; (v) insulin; and/or (vi) transferrin. In a further embodiment, the composition can include (i) basal media; (ii) purified lipoprotein material; (iii) glutamine; (iv) albumin; (v) insulin; and/or (vi) transferrin; and/or (vii) peptone. In another embodiment, the composition can include (i) basal media; (ii) purified lipoprotein material; (iii) glutamine; (iv) albumin; (v) insulin; (vi) transferrin; and/or (vii) fetuin (such as Pedersen). In another embodiment, the composition can include (i) basal media; (ii) purified lipoprotein material; (iii) glutamine; (iv) albumin; (v) insulin; (vi) transferrin; and/or (vii) vitamin E. In a further embodiment, the composition can include (i) serum free media and/or (ii) purified lipoprotein material. In an additional embodiment, the composition can include (i) serum free media and/or (ii) purified lipoprotein material; and/or (iii) albumin.  
         [0078]     In another aspect of the present invention compositions are provided that are useful as a cell culture medium that serves to increase the yield of biological products, such as proteins, produced by the cells cultured in the media. In a further aspect of the present invention, compositions are provided that are useful as a cell culture medium for a variety of cells. In one embodiment, the cell culture media of the present invention can be used for adherent cell culture. In another embodiment, the cell culture media described herein can be used for suspension cell culture.  
         [0079]     In one embodiment, the method includes contacting hybridoma cells with a composition including: (i) basal media; (ii) purified lipoprotein material isolated as; (iii) glutamine; (iv) albumin; (v) insulin; (vi) transferrin; and/or (vii) ethanolamine, and/or maintaining the hybridoma cells under conditions suitable to support cultivation of the hybridoma cells in culture.  
         [0080]     In another embodiment, the present invention is a method of cultivating cancer cells by contacting the cells with compositions that are useful as cell culture medium of the present invention and/or maintaining the cancer cells under conditions suitable to support cultivation of the cancer cells in culture. In a specific embodiment, the method includes contacting cancer cells with a composition including (i) basal media; (ii) purified lipoprotein material; (iii) glutamine; (iv) albumin; (v) insulin; and/or (vi) transferrin.  
       DEFINITIONS  
       [0081]     The terms “cell culture medium,” “culture medium” and “medium formulation” refer to a nutritive solution for culturing or growing cells.  
         [0082]     A “serum-free” medium is a medium that contains no serum (e.g., fetal bovine serum (FBS), horse serum, goat serum, or any other animal-derived serum known to one skilled in the art).  
         [0083]     The term “basal medium” refers to any medium which is capable of supporting growth of cells. The basal medium supplies standard inorganic salts, such as zinc, iron, magnesium, calcium and potassium, as well as trace elements, vitamins, an energy source, a buffer system, and essential amino acids. Suitable basal media include, but are not limited to Dulbecco&#39;s Modified Eagle&#39;s Medium (DMEM), DME/F12, Minimal Essential Medium (MEM), Basal Medium Eagle (BME), RPMI 1640, F-10, F-12, .alpha. Minimal Essential Medium (.alpha.MEM), Glasgow&#39;s Minimal Essential Medium (G-MEM), and Iscove&#39;s Modified Dulbecco&#39;s Medium.  
         [0084]     The term “protein yield” refers to the amount of protein expressed by cultured cells, and can be measured, for example, in terms of grams of protein produced/ml medium. If the protein is not secreted by the cells, the protein can be isolated from the interior of the cells by methods known to those of ordinary skill in the art. If the protein is secreted by the cells, the protein can be isolated from the culture medium by methods known to those of ordinary skill in the art. The amount of protein expressed by the cell can readily be determined by those of ordinary skill in the art. The protein can be a recombinant protein.  
         [0085]     The term “suspension culture” refers to cells in culture in which the majority or all of cells in culture are present in suspension, and the minority or none of the cells in the culture vessel are attached to the vessel surface or to another surface within the vessel (adherent cells). The “suspension culture” can have greater than about 50%, 60%, 65%, 75%, 85%, or 95% of the cells in suspension, not attached to a surface on or in the culture vessel.  
         [0086]     The term “adherent culture” refers to cells in culture in which the majority or all of cells in culture are present attached to the vessel surface or to another surface within the vessel, and the minority or none of the cells in the culture vessel are in suspension. The “adherent culture” can have greater than 50%, 60%, 65%, 75%, 85%, or 95% of the cells adherent.  
         [0087]     The term “purified lipoprotein material” refers to material (i) that can include any lipophilic compound that can be, for example, carried through the plasma by apolipoproteins, including but not limited to cholesteryl esters, unesterified cholesterol, triglycerides, fatty acids and/or phospholipids; and (ii) that is in a higher state of purity than that found naturally in biological materials such as tissue or brain homogenate. In preferred embodiments, the purified lipoprotein material constitutes up to 20, 30, 40, 50, 60, 70, 80 or 90 percent or higher by weight of the material being treated. In one embodiment, the lipoprotein and cholesterol are in substantially pure form, i.e., the material being treated consists essentially of lipoprotein material.  
         [0088]     As used herein, the term “mammal” is meant to include any human or non-human mammal, including but not limited to porcine, ovine, bovine, rodents, ungulates, pigs, sheep, lambs, goats, cattle, deer, mules, horses, monkeys, dogs, cats, rats, and mice.  
         [0000]     I. Cell Culture Media  
         [0089]     The invention provides novel cell culture media compositions and methods to cultivate cells with the novel media. In general, cell culture media contains a base solution or “basal media” into which all of the desired components are added.  
         [0090]     Basal media which can be used in the present invention include but are not limited to Iscove&#39;s Modified Dulbecco&#39;s Medium, RPMI 1640, Minimal Essential Medium-alpha. (MEM-alpha), Dulbecco&#39;s Modification of Eagle&#39;s Medium (DMEM), DME/F12, alpha MEM, Basal Medium Eagle with Earle&#39;s BSS , DMEM high Glucose, with L-Glutamine, DMEM high glucose, without L-Glutamine, DMEM low Glucose, without L-Glutamine, DMEM:F12 1:1, with L-Glutamine,  GMEM  (Glasgow&#39;s MEM),  GMEM  with L-glutamine, Grace&#39;s Complete Insect Medium, Grace&#39;s Insect Medium, without FBS, Ham&#39;s F-10, with L-Glutamine, Ham&#39;s F-12, with L-Glutamine, IMDM with HEPES and L-Glutamine, IMDM with HEPES and without L-Glutamine, IPL-41 Insect Medium, L-15 (Leibovitz)(2×), without L-Glutamine or Phenol Red, L- 15 (Leibovitz), without L-Glutamine, McCoy&#39;s 5A Modified Medium, Medium 199, MEM Eagle, without L-Glutamine or Phenol Red (2×), MEM Eagle-Earle&#39;s BSS, with L-glutamine, MEM Eagle-Earle&#39;s BSS, without L-Glutamine, MEM Eagle-Hanks BSS, without L-Glutamine, NCTC-109, with L-Glutamine, Richter&#39;s CM Medium, with L- Glutamine, RPMI 1640 with HEPES, L-Glutamine and/or Penicillin-Streptomycin, RPMI 1640, with L-Glutamine, RPMI 1640, without L-Glutamine, Schneider&#39;s Insect Medium or any other media known to one skilled in the art.  
         [0091]     The compositions of the present invention can be used to culture a variety of cells. In one embodiment, the medium is used to culture eukaryotic cells such as plant and/or animal cells. The cells can be mammalian cells, fish cells, insect cells, amphibian cells or avian cells. The medium can be used to culture cells selected from the group consisting of MK2.7 cells, PER-C6 cells, CHO cells, HEK 293 cells, COS cells and Sp2/0 cells. MK2.7 (ATCC Catalogue Number CRL 1909) is an anti-murine VCAM IgG1 expressing Hybridoma cell line derived from the fusion of a rat splenocyte and a mouse Sp2/0 myeloma. MK2.7 is a non-adherent cell line that can be grown in serum-free media. Other types of cells can be selected from the group consisting of 5L8 hybridoma cells, Daudi cells, EL4 cells, HeLa cells, HL-60 cells, K562 cells, Jurkat cells, THP-1 cells, Sp2/0 cells; and/or the hybridoma cells listed in Table 2 or any other cell type disclosed herein or known to one skilled in the art.  
         [0092]     Additional mammalian cell types can include, but are not limited to, including primary epithelial cells (e.g., keratinocytes, cervical epithelial cells, bronchial epithelial cells, tracheal epithelial cells, kidney epithelial cells and retinal epithelial cells) and established cell lines and their strains (e.g., 293 embryonic kidney cells, BHK cells, HeLa cervical epithelial cells and PER-C6 retinal cells, MDBK (NBL-1) cells, 911 cells, CRFK cells, MDCK cells, CHO cells, BeWo cells, Chang cells, Detroit 562 cells, HeLa 229 cells, HeLa S3 cells, Hep-2 cells, KB cells, LS 180 cells, LS 174T cells, NCI-H-548 cells, RPMI 2650 cells, SW-13 cells, T24 cells, WI-28 VA13, 2RA cells, WISH cells, BS-C-I cells, LLC-MK.sub.2 cells, Clone M-3 cells, 1-10 cells, RAG cells, TCMK-1 cells, Y-1 cells, LLC-PK.sub.1 cells, PK(15) cells, GH. 1  cells, GH 3  cells, L2 cells, LLC-RC 256 cells, MH.sub.1C 1  cells, XC cells, MDOK cells, VSW cells, and TH-I, B1 cells, or derivatives thereof), fibroblast cells from any tissue or organ (including but not limited to heart, liver, kidney, colon, intestines, esophagus, stomach, neural tissue (brain, spinal cord), lung, vascular tissue (artery, vein, capillary), lymphoid tissue (lymph gland, adenoid, tonsil, bone marrow, and blood), spleen, and fibroblast and fibroblast-like cell lines (e.g., CHO cells, TRG-2 cells, IMR-33 cells, Don cells, GHK-21 cells, citrullinemia cells, Dempsey cells, Detroit 551 cells, Detroit 510 cells, Detroit 525 cells, Detroit 529 cells, Detroit 532 cells, Detroit 539 cells, Detroit 548 cells, Detroit 573 cells, HEL 299 cells, IMR-90 cells, MRC-5 cells, WI-38 cells, WI-26 cells, MiCl.sub.1 cells, CHO cells, CV-1 cells, COS-1 cells, COS-3 cells, COS-7 cells, Vero cells, DBS-FrhL-2 cells, BALB/3T3 cells, F9 cells, SV-T2 cells, M-MSV-BALB/3T3 cells, K-BALB cells, BLO-11 cells, NOR-10 cells, C 3 H/IOTI/2 cells, HSDM.sub.1C 3  cells, KLN205 cells, McCoy cells, Mouse L cells, Strain 2071 (Mouse L) cells, L-M strain (Mouse L) cells, L-MTK (Mouse L) cells, NCTC clones 2472 and 2555, SCC-PSA1 cells, Swiss/3T3 cells, Indian muntac cells, SIRC cells, C II  cells, and Jensen cells, or derivatives thereof).  
         [0093]     The medium disclosed herein can be used to culture cells in suspension or adherent cells. The compositions of the present invention are suitable for either adherent, monolayer or suspension culture, transfection, and cultivation of cells, and for expression of proteins or antibodies in cells in monolayer or suspension culture.  
         [0094]     Cells supported by the medium of the present invention can be derived from any animal, such as a mouse or a human. The cells cultivated in the present media can be normal cells or abnormal cells (i.e., transformed cells, established cells, or cells derived from diseased tissue samples).  
         [0095]     Cell culture can be performed using various culture devices, for example, a fermentor type tank culture device, an air lift type culture device, a culture flask type culture device, a spinner flask type culture device, a microcarrier type culture device, a fluidized bed type culture device, a hollow fiber type culture device, a roller bottle type culture device, a packed bed type culture device or any other suitable devise known to one skilled in the art.  
         [0096]     In another aspect of the present invention compositions are provided that are useful as a cell culture medium that serves to increase the yield of biological products, such as proteins, produced by the cells cultured in the media. In one embodiment, compositions can increase the yield of biological products at least 25%, 30%, 50%, 100%, 200% or 300%. In another embodiment, the biological products produced can be a peptide, such as a therapeutic or diagnostic peptide, polypeptide, protein, monoclonal antibody, immunoglobulin, cytokine (such as interferon, for example, interferon alpha, beta or gamma), integrin, antigen, growth factor, cell cycle protein, hormone, neurotransmitter, receptor, fusion peptide, blood protein and/ or chimeric protein. The biological product can also be an IgG, IgM, IgE, IgA immunogliobulin, a signle chain antibody or fragment thereof, such as a sFv fragment, a linked antibody fragment, and/or a humanized antibody.  
         [0000]     II. Compositions to Reduce the Use of Serum  
         [0097]     Traditionally, serum has been used to supplement classical basal media to increase cell growth and protein yield. In a first embodiment, the composition can include (i) basal media; (ii) purified lipoprotein material; and (iii) a reduced concentration of serum, such as fetal bovine serum (FBS), relative to the use of serum alone to supplement basal media.  
         [0098]     In embodiments of the present invention, the purified lipoprotein material can be derived from serum or plasma obtained from a mammal. In one embodiment, the purified lipoprotein material can be a cholesterol-rich fraction, optionally in association with low density lipoprotein (LDL) and/ or high density lipoprotein (HDL). In another embodiment, the purified lipoprotein material can be derived from bovine, horse, sheep, pig or human serum or plasma. In a further embodiment, the purified lipoprotein material can contain cholesterol, such as approximately 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 30, 40 or 50 grams per liter, more particularly, 9, 10 or 11, grams per liter of cholesterol as determined, for example, by enzymatic assay; protein, such as approximately between 10 and 20 or 5 and 30 grams per liter, more particularly, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 35, 40, 45 or 50 grams per liter of protein, and/ or approximately between 0 and 10 or between 0 and 6 EU endotoxin per milligram of cholesterol, more particularly, 0, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 7, 8, 9, 10, 15, 20, 25, or 30 EU endotoxin per milligram of cholesterol, such as determined, for example, by limulus amebocyte lysate. In another embodiment, the pH of the purified lipoprotein material can be between approximately 7 and 8, more particularly, between about 7.0 and 8.4, for example, about 6.5, 6.6, 6.7, 6.8, 6.9, 7.0, 7.1, 7.2, 7.3, 7.4, 7.5, 7.6, 7.7, 7.8, 7.9, 8.0, 8.1, 8.2, 8.3, 8.4, 8.5, 8.6, 8.7, 8.8, 8.9, 9.0, 9.5, or 10. In other embodiments, the purified lipoprotein material does not contain detectable levels of contaminants or undesired materials, for example, immunoglobulin G (IgG), microbes, mycoplasm, and/or viral agents.  
         [0099]     Traditionally, in cell culture, basal media is supplemented with about 10%, 15%, 20%, 25%, 30% or more serum, such as FBS. In this embodiment, basal media is supplemented with less than about 10%, 15%, 20%, 25%, or 30% serum, and more particularly, less than about 9.5, 9, 8.5, 8, 7.5, 7, 6.5, 6, 5.5, 5, 4.5, 4, 3.5, 3, 2.5, 2, 1.5, 1, 0.75, 0.5, or 0.25% serum, such as FBS, in combination with a purified lipoprotein material. In one embodiment, between 0.1 and 5%, 0 and 10% or 1 and 5% purified lipoprotein material can be used in the composition. In another embodiment, approximately 0.2, 0.3., 0.4, 0.5, 0.6., 0.7, 0.75, 0.8, 0.9, 1.0, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 7, 8, 9, 10, 20 or 30% purified lipoprotein material can used. In further embodiments, 0.1 to 1.0%, 0.1 to 0.5, 0.5 to 1.0%, 0.5 to 1.5%, 0.1 to 2%, 1 to 2%, 1 to 5%, 5 to 10% or 10 to 20% purified lipoprotein material can be used.  
         [0000]     III. Compositions to Replace Serum  
         [0100]     The biochemical complexity of serum, such as FBS, can potentially complicate the downstream processing of the proteins of interests. The present invention also provides a unique formulation of complete media without the use of serum.  
         [0101]     According to this aspect of the invention, the composition can include (i) basal media; (ii) purified lipoprotein material; (iii) insulin; iv) albumin; (v) sodium selenite; and (vi) transferrin.  
         [0102]     The composition can include any amount of BSA that achieves the desired effect, including but not limited to approximately 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5 or 9 mg/ml albumin, such as BSA. In one embodiment, the composition can include 1 to 3, 1 to 5, 2 to 4, 2 to 7, 3 to 6, 5 to 9, 5 to 8, or 2 to 8 mg/ml albumin, such as BSA. The albumin can be bovine serum albumin (BSA) or human serum albumin (HSA). The albumin can be an “albumin substitute”, which can be any compound which may be used in place of bovine serum albumin (e.g., human serum albumin (BSA) or AlbuMAX.RTM.I) in the supplement of the invention to give substantially similar results as albumin. Albumin substitutes may be any protein or polypeptide source. Examples of such protein or polypeptide samples include but are not limited to bovine pituitary extract, plant hydrolysate (e.g., rice hydrolysate), fetal calf albumin (fetuin), egg albumin, human serum albumin (HSA), or another animal-derived albumins, chick extract, bovine embryo extract, AlbuMAX.RTM.I, and AlbuMAX.RTM. II. In a specific embodiment, the composition can include 3.5 to 5.0 mg/ml, specifically, 4 mg/ml, BSA. The BSA can be a cell culture grade BSA, such as available from Serologicals, Inc.  
         [0103]     The composition can also include any amount of transferrin that achieves the desired effect, including but not limited to approximately 3.0, 3.5, 4.0, 4.5, 5.0, 5.5, 6.0, 7.0, 10, 15 or 20 ug/ml transferrin. In one embodiment, the composition can include 2.5 to 3.0, 3.0 to 4.0, 3.0 to 5.0, 3.0 to 6.0, 4.0 to 8.0, or 6.0 to 10.0 ug/ml transferrin. In a specific embodiment, the composition can include 2 to 4 mg/ml, specifically 2.5 mg/ml, transferrin.  
         [0104]     A transferrin substitute can also be used. A “transferrin substitute” refers to any compound which can replace transferrin and provides substantially similar results as transferrin. Examples of transferrin substitutes include but are not limited to any iron chelate compound, such as including, but not limited to, iron chelates of ethylenediaminetetraacetic acid (EDTA), ethylene glycol-bis(beta-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA), deferoxamine mesylate, dimercaptopropanol, diethylenetriamine-pentaacetic acid (DPTA), and trans-1,2-diaminocyclohexane-N,N,N′,N′-tetraacetic adic (CDTA), as well as a ferric citrate chelate and a ferrous sulfate chelate. The transferrin can be iron saturated transferring, such as human transferrin. The transferring can be a cell culture grade transferrin, such as that available from Serologicals, Inc.  
         [0105]     The composition can also include any amount of insulin that achieves the desired effect, including but not limited to approximately 5.5, 6.0, 6.5, 7.0, 7.5, 8.0, 8.5, 9.0, 9.5, 10, 10.5, 11, 11.5, 12, 15 or 20 ug/ml insulin. In one embodiment, the composition can include 5 to 7, 5.5 to 6, 7 to 10, 9 to 11, 8 to 12 or 10 to 15 ug/ml insulin.  
         [0106]     An insulin substitute can also be used. The term “insulin substitute” refers to any zinc containing compound which may be used in place of insulin that provides substantially similar results as insulin. Examples of insulin substitutes include but are not limited to zinc chloride, zinc nitrate, zinc bromide, and zinc sulfate. Additional insulins are known to those of ordinary skill in the art, see, for example, Gilman, A. G. et al., Eds., The Pharmacological Basis of Therapeutics, Pergamon Press, New York, 1990, pp. 1463-1495. The insulin can be zinc insulin or human zinc insulin. The insulin can be cell culture grade insulin, such as available from Serologicals, Inc.  
         [0107]     The composition can also include any amount of sodium selenite that achieves the desired result, including but not limited to approximately 1, 2, 3, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 6.7, 7.0, 7.5, 8.0, 9.0, 10, 15 or 20 ug/L sodium selenite. In one embodiment, the composition can include 1 to 5, 5.5 to 15, 6.0 to 7.0 or 6.0 to 10 ug/L sodium selenite.  
         [0108]     The composition can include any amount of purified lipoprotein materialthat achieves the desired result, including but not limited to 0.2, 0.3., 0.4, 0.5, 0.6, 0.7, 0.75, 0.8, 0.9, 1.0, 1.5 or 2% purified lipoprotein materialin basal media. In one embodiment, 0.1 to 1.0%, 0.1 to 0.5, 0.5 to 1.0%, 0.5 to 1.5%, 0.1 to 2%, 1 to 2%, 1 to 5%, 5 to 10% or 10 to 20% purified lipoprotein materialcan be used in the composition.  
         [0109]     In one specific embodiment, approximately 4 mg/ml BSA; approximately 5.5 ug/ml transferrin; approximately 10 ug/ml insulin; approximately 6.7 ug/L sodium selenite; and approximately 0.75% purified lipoprotein materialin basal media.  
         [0110]     In another specific embodiment, the composition can include: approximately 3 to 5 mg/ml BSA, approximately 4.5 to 6.5 ug/ml transferrin, approximately 9 to 11 ug/ml insulin, approximately 6.0 to 7.0 ug/L sodium selenite and approximately 0.25 to 1.0% purified lipoprotein materialin basal media.  
         [0000]     IV. Compositions to Boost the Performance of Serum-Free Media  
         [0111]     Other types of serum-free edia have been developed to substitute for the use of serum in cell culture. Compositions of the present invention, such as those that contain a purified lipoprotein material, can be used as a supplemented to further boost the growth of cells and increase the yield of products produced  
         [0112]     In one aspect, the composition can include (i) serum free media and (ii) purified lipoprotein material. In one embodiment, the serum free media is one of the media listed in Table 1. In another embodiment, the serum free media is either Hybridoma Media, animal component free or Ex-Cell (JRH Biosceinces, Inc.). In another embodiment, approximately 0.2, 0.3., 0.4, 0.5, 0.6, 0.7, 0.75, 0.8, 0.9, 1.0, 1.5 or 2% purified lipoprotein material is used. In a further embodiment, 0.1 to 1.0%, 0.1 to 0.5, 0.5 to 1.0%, 0.5 to 1.5%, 0.1 to 2%, 1 to 2%, 1 to 5%, 5 to 10% or 10 to 20% purified lipoprotein material can be used in the composition.  
         [0113]     In another aspect, the composition can include (i) serum free media and (ii) purified lipoprotein material; and (iii) albumin. In one embodiment, the serum free media is one of the media listed in Table 1. In another embodiment, the serum free media is either Hybridoma Media, animal component free or Ex-Cell (JRH Biosceinces, Inc.). In a further embodiment, the composition can include approximately 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 2, 3, 4, 5, or 10% albumin, such as bovine serum albumin (BSA) or other types of albumin as described above. In another embodiment, approximately 0.2, 0.3., 0.4, 0.5, 0.6, 0.7, 0.75, 0.8, 0.9, 1.0, 1.5 or 2% purified lipoprotein material is used. In a further embodiment, 0.1 to 1.0%, 0.1 to 0.5, 0.5 to 1.0%, 0.5 to 1.5%, 0.1 to 2%, 1 to 2%, 1 to 5%, 5 to 10% or 10 to 20% purified lipoprotein material can be used in the composition.  
         [0114]     In a specific embodiment, the composition can include ExCell, approximately 0.75% purified lipoprotein material and approximately 0.5% BSA. In another specific embodiment, the composition can include Hybridoma Medium, Animal Component-free, approximately 0.5% purified lipoprotein material and approximately 0.2% BSA.  
         [0000]     V. Cell Culture Compositions and Methods to Enhance the Growth and Performance of Cells  
         [0115]     The compositions and methods of the present invention can also be used to enhance the growth and product yield of hybridomas in cell culture.  
         [0116]     In one aspect of the invention, the composition can include (i) basal media; (ii) purified lipoprotein; (iii) glutamine; (iv) albumin; (v) insulin; (vi) transferrin; (vii) ethanolamine. In one embodiment, the basal media can be DMEM. In one embodiment, between 0.1 and 5% purified lipoprotein material can be used in the composition. In another embodiment, approximately 0.1, 0.2, 0.3., 0.4, 0.5, 0.6., 0.7, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9 3, 3.1, 3.2, 3.4, 3.5, 4, 5, 10, 15, 20, 25 or 30% purified lipoprotein material can be used. In further embodiments, 0.1 to 1.0%, 0.1 to 0.5, 0.5 to 1.0%, 0.5 to 1.5%, 0.1 to 2%, 1 to 2%, 1.5 to 2.0%, 2.0 to 2.5%, 1 to 5%, 5 to 10% or 10 to 20% purified lipoprotein material can be used. In a specific embodiment, the composition can include approximately 2.0% purified lipoprotein material.  
         [0117]     The composition can include any amount of glutamine that achieves the desired effect, including but not limited to approximately 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 20, 25 or 30 mM glutamine. In one embodiment, the composition can include 2 to 3, 3 to 4, 4 to 5, or 5 to 6 mM glutamine, particularly approximately 4 mM glutamine.  
         [0118]     The composition can include any amount of albumin that achieves the desired effect, including but not limited to up to approximately 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 20, 25%; 3 to 5, 5 to 8% albumin. In one embodiment, the composition contains from approximately 0.2 to 0.4, 0.4 to 0.6, 0.6 to 0.9%, particularly approximately 0.5% albumin. In one embodiment, the albumin can be bovine serum albumin (BSA) or human serum albumin (HSA). In a specific embodiment, the composition can include 0.5% BSA. The albumin can be an “albumin substitute”, which can be any compound which may be used in place of bovine serum albumin (e.g., human serum albumin (BSA) or AlbuMAX.RTM.I) in the supplement of the invention to give substantially similar results as albumin. Albumin substitutes may be any protein or polypeptide source. Examples of such protein or polypeptide samples include but are not limited to bovine pituitary extract, plant hydrolysate (e.g., rice hydrolysate), fetal calf albumin (fetuin), egg albumin, human serum albumin (HSA), or another animal-derived albumins, chick extract, bovine embryo extract, AlbuMAX.RTM.I, and AlbuMAX.RTM. II. The BSA can be a cell culture grade BSA, such as available from Serologicals, Inc.  
         [0119]     The composition can also include any amount of insulin that achieves the desired effect, including but not limited to approximately 1, 2, 3, 4, 5, 6, 7, 8, 9, 9.5, 10, 10.5, 11, 11.5, 12, 13, 14, 15, 16, 17, 18, 19, 20, 25, 30, 40% or 16 to 18, 18 to 20 mg/L insulin. In one embodiment, the composition can include 7 to 9, 9 to 11, 11 to 13, specifically about or approximately 10 mg/L insulin. An insulin substitute can also be used. The term “insulin substitute” refers to any zinc containing compound which may be used in place of insulin that provides substantially similar results as insulin. Examples of insulin substitutes include but are not limited to zinc chloride, zinc nitrate, zinc bromide, and zinc sulfate. Additional insulins are known to those of ordinary skill in the art, see, for example, Gilman, A. G. et al., Eds., The Pharmacological Basis of Therapeutics, Pergamon Press, New York, 1990, pp. 1463-1495. In one embodiment, the insulin can be zinc insulin or human zinc insulin. The insulin can be cell culture grade insulin, such as available from Serologicals, Inc. In a specific embodiment, the insulin is human recombinant insulin such as that available from Serologicals.  
         [0120]     The composition can also include any amount of transferrin that achieves the desired effect, including but not limited to approximately 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 0.95, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 3, 4, 5, 10, or 15 mg/L transferrin; 3 to 5, or 5 to 7 mg/L transferrin. In one embodiment, the composition can include 0.5 to 1.0, 1.0 to 1.5, 1.5 to 2.0 mg/L transferrin. In a specific embodiment, the composition can include approximately 1.0 mg/L transferrin. A transferrin substitute can also be used. A “transferrin substitute” refers to any compound which can replace transferrin and provides substantially similar results as transferrin. Examples of transferrin substitutes include but are not limited to any iron chelate compound, such as including, but not limited to, iron chelates of ethylenediaminetetraacetic acid (EDTA), ethylene glycol-bis(beta-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA), deferoxamine mesylate, dimercaptopropanol, diethylenetriamine-pentaacetic acid (DPTA), and trans-1,2-diaminocyclohexane-N,N,N′,N′-tetraacetic adic (CDTA), as well as a ferric citrate chelate and a ferrous sulfate chelate. The transferrin can be iron saturated transferring, such as human transferrin. The transferring can be a cell culture grade transferrin, such as that available from Serologicals, Inc. In a specific embodiment, the transferring is human holo-transferrin, such as that available from Serologicals, Inc.  
         [0121]     The composition can include any amount of ethanolamine that exerts the desired effect, including but not limited to approximately 1, 2, 3, 4, 5, 6, 7, 8, 8.5, 9, 9.1, 9.2, 9.3, 9.4, 9.5, 9.6, 9.7, 9.8, 9.9, 10, 10.1, 10.2, 10.3, 10.4, 10.5, 10.6, 10.7, 10.8, 10.9, 11, 11.5, 12, 13 to 15, or 15 to 20 μM ethanolamine. In one embodiment, the composition can include 9.0 to 9.5, 9.5 to 10, 10 to 10.5, or 10.5 to 11 μM ethanolamine. In a specific embodiment, the composition can include approximately 10 μM ethanolamine.  
         [0122]     In other embodiments, the cell culture media can contain basal media, approximately between 1 and 4, 2 and 4, 1 and 3, 1 and 5, 0.5 and 5, 0.5 and 4.5, 3 and 5, 3.5 and 4.5 mM glutamine, approximately between 0.2 and 1.0%, 0.1 and 1.0%, 0.5 and 1.0%, 0.3 and 1.5%; 0.2 and 5%; 0.2 and 3% and 0.3 and 2% albumin; approximately between 1 and 10 mg/L, 8 and 12 mg/L, 5 and 15 mg/L or greater than 25 mg/L insulin; approximately between 0.5 and 9.5, 0.5 and 9.7, 0.5 and 1.5, 0.5 and 5, 0.5 and 7, and 0.5-9 mg/L transferrin; approximately 1 and 10 uM, 8 and 12 uM, 5 and 15 uM or greater than 25 uM ethanolamine, and approximately between 0.5% and 3%, 0.5% and 5%, 1% and 5%, 0.5% and 0.9%, 1.5% and 2.5%; and 1-3% of a purified lipoprotein material. In one specific embodiment, the composition of the present invention can include approximately 4 mM glutamine, approximately 2% purified lipoprotein material, approximately 0.5% BSA, approximately 10 mg/L insulin, approximately 1 mg/L transferrin and approximately 10 micromolar ethanolamine.  
         [0123]     The present invention also includes a method of culturing cells using a involving contacting the cells with a composition described herein, including, but not limited to: (i) basal media; (ii) purified lipoprotein material; (iii) glutamine; (iv) albumin; (v) insulin; (vi) transferrin; and/ or (vii) ethanolamine, and maintaining the cells under conditions suitable to support cultivation of the cells in culture. In one embodiment of the present invention, the cells are eukaryotic cells, such as plant or animal cells or any other cell described herein. In a particular embodiment, the cells are MK2.7 cells, HEK 293 cells, CHO cells, PER-c6 cells, 5L8 cells, COS cells and Sp2/o cells.  
         [0124]     In one embodiment, the preset invention provides a method of culturing hybridoma cells involving contacting the cells with a composition including (i) basal media; (ii) purified lipoprotein material; (iii) glutamine; (iv) albumin; (v) insulin; (vi) transferrin; and (vii) ethanolamine, and maintaining the cells under conditions suitable to support cultivation of the hybridoma cells in culture. In one embodiment, the hybridoma is one of the hybridomas listed in Table II. In a particular embodiment, the hybridoma is MK2.7.4. In a further embodiment, the hybridoma is 5L8.  
         [0125]     In a specific embodiment, the preset invention is method of culturing hybridoma cells involving contacting the cells with a composition including (i) basal media; (ii) approximately 2% purified lipoprotein material; (iii) approximately 4 mM glutamine; (iv) approximately 0.5% BSA; (v) approximately 10 mg/L insulin; (vi) approximately 1 mg/L transferrin; and (vii) approximately 10 micromolar ethanolamine, and maintaining the cells under conditions suitable to support cultivation of the hybridoma cells in culture.  
         [0126]     In another aspect of the invention, the composition can include (i) basal media; (ii) purified lipoprotein material; (iii) glutamine; (iv) albumin; (v) insulin; and (vi) transferrin. In one embodiment, the basal media is RPMI 1460. In one embodiment, between 0.01 and 5% purified lipoprotein material is used in the composition. In another embodiment, approximately 0.02, 0.03., 0.04, 0.05, 0.06., 0.07, 0.8, 0.09, 1, 1.5, 2, 2.5, or 3% purified lipoprotein material can be used. In further embodiments, 0.01 to 0.05 %, 0.05 to 0.1%, 0.1 to 0.15%, 0.2 to 0.3% purified lipoprotein material can be used. In other embodiments, any amount of purified lipoprotein material as disclosed herein can be used. In a specific embodiment, the composition can include approximately 0.1% purified lipoprotein material.  
         [0127]     The composition can include any amount of glutamine that achieves the desired effect, including but not limited to approximately 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 mM glutamine. In one embodiment, the composition can include 2 to 3, 3 to 4, 4 to 5, 2 to 10, 1 to 20, 3 to 10, 3 to 6, or 5 to 6 mM glutamine, specifically approximately 4 mM glutamine.  
         [0128]     The composition can include any amount of albumin that achieves the desired effect, including but not limited to up to approximately 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.5, 2, 2.5, 3, 3.5, 3.5 to 5, 5 to 10, 10 to 20% albumin. In one embodiment, the composition contains from 0.5 to 1, 1 to 1.5, 1.5 to 2, specifically approximately 0.5% albumin. The albumin can be bovine serum albumin (BSA) or human serum albumin (HSA). In a specific embodiment, the composition can include 1.0% BSA. The albumin can be an “albumin substitute”, which can be any compound which may be used in place of bovine serum albumin (e.g., human serum albumin (BSA) or AlbuMAX.RTM.I) in the supplement of the invention to give substantially similar results as albumin. Albumin substitutes may be any protein or polypeptide source. Examples of such protein or polypeptide samples include but are not limited to bovine pituitary extract, plant hydrolysate (e.g., rice hydrolysate), fetal calf albumin (fetuin), egg albumin, human serum albumin (HSA), or another animal-derived albumins, chick extract, bovine embryo extract, AlbuMAX.RTM.I, and AlbuMAX.RTM. II. The BSA can be a cell culture grade BSA, such as available from Serologicals, Inc.  
         [0129]     The composition can also include any amount of insulin that achieves the desired effect, including but not limited to approximately 1, 2, 3, 4, 5, 6, 7, 8, 9, 9.5, 10, 10.5, 11, 11.5, 12, 13, 14, 15, 16 to 18, 18 to 20 mg/L insulin. In one embodiment, the composition can include 7 to 9, 9 to 11, 11 to 13, including approximately 10 mg/L insulin. An insulin substitute can also be used. The term “insulin substitute” refers to any zinc containing compound which may be used in place of insulin that provides substantially similar results as insulin. Examples of insulin substitutes include but are not limited to zinc chloride, zinc nitrate, zinc bromide, and zinc sulfate. Additional insulins are known to those of ordinary skill in the art, see, for example, Gilman, A. G. et al., Eds., The Pharmacological Basis of Therapeutics, Pergamon Press, New York, 1990, pp. 1463-1495. The insulin can be zinc insulin or human zinc insulin. The insulin can be cell culture grade insulin, such as available from Serologicals, Inc. In a specific embodiment, the insulin is human recombinant insulin such as that available from Serologicals.  
         [0130]     The composition can also include any amount of transferrin that achieves the desired effect, including but not limited to approximately 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 0.95, 1, 1.5, 2, 2.5, 3 to 5, or 5 to 7 mg/L transferrin. In one embodiment, the composition can include 0.5 to 1.0, 1.0 to 1.5, 1.5 to 2.0 mg/L transferring. In a specific embodiment, the composition can include approximately 1.0 mg/L transferrin. A transferrin substitute can also be used. A “transferrin substitute” refers to any compound which can replace transferrin and provides substantially similar results as transferrin. Examples of transferrin substitutes include but are not limited to any iron chelate compound, such as including, but not limited to, iron chelates of ethylenediaminetetraacetic acid (EDTA), ethylene glycol-bis(beta-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA), deferoxamine mesylate, dimercaptopropanol, diethylenetriamine-pentaacetic acid (DPTA), and trans-1,2-diaminocyclohexane-N,N,N′,N′-tetraacetic adic (CDTA), as well as a ferric citrate chelate and a ferrous sulfate chelate. The transferrin can be iron saturated transferrin, such as human transferrin. The transferrin can be a cell culture grade transferrin, such as that available from Serologicals, Inc. In a specific embodiment, the transferring is human holo-transferrin, such as that available from Serologicals, Inc.  
         [0131]     In one specific embodiment, the composition of the present invention can include approximately 4 mM glutamine, approximately 0.1% purified lipoprotein material, approximately 1% BSA, approximately 10 mg/L insulin, and approximately 1 mg/L transferrin.  
         [0132]     In another embodiment, the composition can include (i) basal media; (ii) purified lipoprotein material; (iii) glutamine; (iv) albumin; (v) insulin; (vi) transferrin; and (vii) peptone. The composition can include any amount of peptone that exerts the desired effect, including but not limited to approximately 0.01, 0.02, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.1, 0.2, 0.3, 0.4, 0.5, 1, 2 or 3 % peptone. In one embodiment, the composition can include approximately 0.05 to 0.1, 0.1 to 0.2, 0.2 to 0.3, 0.3 to 0.5% peptone. In one specific embodiment, the composition of the present invention can include approximately 4 mM glutamine, approximately 0.1% purified lipoprotein material, approximately 1% BSA, approximately 10 mg/L insulin, approximately 1 mg/L transferrin, and approximately 0.5% peptone.  
         [0133]     In further embodiment, the composition can include (i) basal media; (ii) purified lipoprotein material; (iii) glutamine; (iv) albumin; (v) insulin; (vi) transferrin; and (vii) fetuin. The composition can include any amount of fetuin that exerts the desired effect, including but not limited to approximately 2, 3, 4, 5, 6, 7, 8, 9, 10, 10.5, 11, 11.5, 12, 12.5, 13, 13.5, 14, 14.5, 15, 16 to 18, 18 to 20 micrograms/ml of fetuin. In one embodiment of the invention, fetuin is Pedersen&#39;s fetuin. In a particular embodiment, the composition contains approximately 8 to 10, 10 to 12, 12 to 14 micrograms/mL Pedersen&#39;s fetuin, specifically approximately 12.5 μg/mL Pedersen&#39;s fetuin, such as that available from Serologicals, Inc. In a specific embodiment of the present invention, the composition can include approximately 4 mM glutamine, approximately 0.1% purified lipoprotein material, approximately 1% BSA, approximately 10 mg/L insulin, approximately 1 mg/L transferrin, and approximately 12.5 μg/mL of Pedersen&#39;s fetuin.  
         [0134]     In yet another embodiment, the composition can include (i) basal media; (ii) purified lipoprotein material; (iii) glutamine; (iv) albumin; (v) insulin; (vi) transferrin; and (vii) vitamin E. The composition can include any amount of vitamin E that exerts the desired effect, including but not limited to approximately 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 μM vitamin E. In one embodiment, the composition can include approximately 3 to 5, 5 to 8 or 8 to 10 μM vitamin E, specifically approximately 5 μM vitamin E. In a specific embodiment of the present invention, the composition can include approximately 4 mM glutamine, approximately 0.1% purified lipoprotein material, approximately 1% BSA, approximately 10 mg/L insulin, approximately 1 mg/L transferrin, and approximately 5 μM vitamin E  
         [0135]     In a preferred embodiment, the composition of the present invention can include (i) basal media; (ii) purified lipoprotein material; (iii) glutamine; (iv) albumin; (v) insulin; (vi) transferrin; and (vii) peptone; (viii) fetuin; and (ix) vitamin E. In one specific embodiment, the composition can include approximately 4 mM glutamine, approximately 0.1% purified lipoprotein material, approximately 1% BSA, approximately 10 mg/L insulin, approximately 1 mg/L transferrin, approximately 0.1% peptone, approximately 12.5 micrograms/ml fetuin, and approximately 5 micromolar vitamin E.  
         [0136]     The composition can be used to boost the growth and production of cells in culture, including eukaryotic cells. Thus, one aspect of the present invention is a method of cultivating cells comprising contacting the cells with a composition including: (i) basal media; (ii) purified lipoprotein material; (iii) glutamine; (iv) albumin; (v) insulin; (vi) transferrin.  
         [0137]     A further aspect of the present invention is a method of cultivating cancer cells comprising contacting the cells with a composition of the present invention, including, but not limited to: (i) basal media; (ii) the purified lipoprotein material (iii) glutamine; (iv) albumin; (v) insulin; and/ or (vi) transferrin.  
         [0138]     In one embodiment, the present invention provides a method of cultivating cancer cells comprising contacting the cells with a composition described herein, such as including: (i) basal media; (ii) purified lipoprotein material; (iii) glutamine; (iv) albumin; (v) insulin; (vi) transferrin; and/ or (vii) peptone.  
         [0139]     In another embodiment, present invention is a method of cultivating cancer cells comprising contacting the cells with a composition including: (i) basal media; (ii) purified lipoprotein material; (iii) glutamine; (iv) albumin; (v) insulin; (vi) transferrin; and (vii) fetuin. In a specific embodiment, the fetuin is Pedersens fetuin.  
         [0140]     In one embodiment, present invention is a method of cultivating cancer cells comprising contacting the cells with a composition including: (i) basal media; (ii) purified lipoprotein material; (iii) glutamine; (iv) albumin; (v) insulin; (vi) transferrin; and (vii) vitamin E.  
         [0141]     In a further embodiment, the present invention is a method of cultivating cancer cells comprising contacting the cells with a composition including: (i) basal media; (ii) purified lipoprotein material; (iii) glutamine; (iv) albumin; (v) insulin; (vi) transferrin; (vii) peptone; and/ or (viii) fetuin.  
         [0142]     In yet another embodiment, the present invention is a method of cultivating cancer cells comprising contacting the cells with a composition including: (i) basal media; (ii) purified lipoprotein material; (iii) glutamine; (iv) albumin; (v) insulin; (vi) transferrin; (vii) peptone; and/ or (viii) vitamin E.  
         [0143]     In another embodiment, the present invention is a method of cultivating cancer cells comprising contacting the cells with a composition including: (i) basal media; (ii) purified lipoprotein material; (iii) glutamine; (iv) albumin; (v) insulin; (vi) transferrin; (vii) vitamin E; and/ or (viii) fetuin.  
         [0144]     In a specific embodiment, the method of the present invention is a method of cultivating cancer cells comprising contacting the cells with a composition including: (i) basal media; (ii) purified lipoprotein material; (iii) glutamine; (iv) albumin; (v) insulin; (vi) transferrin; (vii) peptone; (viii) Pedersen&#39;s fetuin; and/ or (ix) vitamin E.  
         [0145]     In a preferred embodiment, the method of the present invention is a method of cultivating cancer cells comprising contacting the cells with a composition including: (i) basal media; (ii) approximately 0.1% purified lipoprotein material; (iii) approximately 4 mM glutamine; (iv) approximately 1% BSA; (v) approximately 10 mg/L insulin; (vi) approximately 1 mg/: transferrin; (vii) approximately 0.1% peptone; (viii) approximately 12.5 μg/ml Pedersen&#39;s fetuin; and/ or (ix) approximately 5 μM vitamin E.  
         [0146]     Any number of cancer cell lines are familiar to those skilled in the art. Representative examples of cancer cell lines that can be cultivated by the method of the present invention include but are not limited to the following cancer cell lines: human myeloma (e.g., KMM-1, KMS-11, KMS-12-PE, KMS-12-BM, KMS-18, KMS-20, KMS-21-PE, U266, RPMI8226); human breast cancer (e.g., KPL-1, KPL4, MDA-MB-231, MCF-7, KPL-3C, T47D, SkBr3, HS578T, MDA4355, Hs 606 (CRL-7368), Hs 605.T (CRL-7365) Hs 742.T (CRL-7482), BT474, HBL-100, HCC202, HCC1419, HCC1954, MCF7, MDA-361, MDA436, MDA453, SK-BR-3, ZR-75-30, UACC-732, UACC-812, UACC-893, UACC-3133, MX-1 and EFM-192A); ductal (breast) carcinoma (e.g., HS 57HT (HTB-126), HCC1008 (CRL-2320), HCC1954 (CRL-2338; HCC38 (CRL-2314), HCC1143 (CRL-2321), HCC1187 (CRL-2322), HCC1295 (CRL-2324), HCC1599 (CRL-2331), HCC1937 (CRL-2336), HCC2157 (CRL-2340), HCC2218 (CRL-2343), Hs574.T (CRL-7345), Hs 742.T (CRL-7482); skin cancer (e.g., COLO 829 (CRL-1974), TE 354.T (CRL-7762), Hs 925.T (CRL-7677)); human prostate cancer (e.g., MDA PCa 2a and MDA PCa 2b); bone cancer (e.g., Hs 919.T (CRL-7672), Hs 821.T (CRL-7554), Hs 820.T (CRL-7552), Hs 704.T (CRL-7444), Hs 707(A).T (CRL-7448), Hs 735.T (CRL-7471), Hs 860.T (CRL-7595), Hs 888.T.(CRL-7622); Hs 889.T (CRL-7626); Hs 890.T (CRL-7628), Hs 709.T (CRL-7453)); human lymphoma (e.g., K562); human cervical carcinoma (e.g., HeLA); lung carcinoma cell lines (e.g., H125, H522, H1299, NCI-H2126 (ATCC CCL-256), NCI-H1672 (ATCC CRL-5886), NCI-2171 (CRL-5929); NCI-H2195 (CRL05931); lung adenocarcinoma (e.g., NCI-H1395 (CRL-5856), NCI-H1437 (CRL-5872), NCI-H2009 (CRL-5911), NCI-H2122 (CRL-5985), NCI-H2087 (CRL-5922); metastatic lung cancer (e.g., bone) (e.g., NCI-H209 (HTB-172); colon carcinoma cell lines (e.g., LN235, DLD2, Colon A, LIM2537, LIM1215, LIM1863, LIM1899, LIM2405 LIM2412 , SK-CO1 (ATCC HTB-77), HT29 (ATCC HTB38), LoVo (ATCC CCL-229), SW1222 (ATCC HB-11028), and SW480 (ATCC CCL-228); ovarian cancer (e.g., OVCAR-3 (ATCC HTB-161) and SKOV-3 (ATCC HTB-77); mesothelioma (e.g., NCI-h2052 (CRL-5915); neuroendocrine carcinoma (e.g., HCI-H1770 (e.g., CRL-5893); gastric cancer (e.g., LIM1839); glioma (e.g., T98, U251, LN235); head and neck squamous cell carcinoma cell lines (e.g., SCC4, SCC9 and SCC25); medulloblastoma (e.g., Daoy, D283 Med and D341 Med); testicular non-seminoma (e.g., TERA1); prostate cancer (e.g., 178-2BMA, Du145, LNCaP, and PC-3). Other cancer cell lines are well known in the art.  
       EXAMPLES  
     Example 1  
       [0000]     Method to Obtain Cholesterol-Rich Fraction From Bovine Serum  
         [0147]     Starting material for a process according to the present invention can be maintained at a temperature of about 0° C. to about 50° C. Typically, the temperature is maintained at about 2° C. to about 15° C. A process according to the present invention can begin by subjecting the starting material to filtration. The filtration can be carried out utilizing one or more filtration steps. According to one embodiment, two filtration steps are sequentially utilized with filters having a nominal porosity of about 5 μ and about 1 μ. Any suitable filter in this range can be utilized.  
         [0148]     If the starting material is serum, it is preferred to add a soluble salt, such as sodium citrate, to an ionic strength of about 0.25 to about 1. Other suitable salts include sodium chloride, sodium phosphate, potassium phosphate, ammonium sulfate and sodium sulfate. The addition of a soluble salt to the above concentration will increase the amount of cholesterol-rich fraction adsorbed in the subsequent silica adsorption step. Bovine or human plasma, for example, is normally collected by a method, which can include addition of citrate as an anti-coagulant. This salt concentration is usually sufficient for the adsorption step and no additional salt is needed. After adding the soluble salt, the solution can be mixed. Typically, the solution is mixed for about 30 minutes.  
         [0149]     After addition of sodium citrate, other materials that can facilitate processing can be added to the starting material and any added soluble salt(s). According to one example, polyethyleneglycol (PEG) can be added to the filtered starting material. PEG having a range of molecular weights can be utilized. According to one example, PEG having an average molecular weight of about 3350 is utilized. However, PEG having greater or lesser molecular weights can also be utilized. Along these lines, PEG having an average molecular weight of about 6000 could be utilized. One of ordinary skill in the art, once aware of the disclosure contained herein would be able to determine the molecular weight of PEG to utilize with out undue experimentation. The PEG can be added in an amount of about 10 grams to about 15.6 grams for each liter of filtered starting material and sodium citrate, if utilized. After addition of the PEG the solution can be mixed. Typically, the solution is mixed for about 30 minutes, although shorter or longer mixing times can be utilized. While the addition of PEG can facilitate the purification process, it is not necessary.  
         [0150]     After addition of the PEG, if utilized, the pH can be adjusted to a slightly acidic value. Along these lines, the pH can be adjusted to a value of about 5 to about 8. Typically, the pH is adjusted to a value of about 5.8 to about 6.2  
         [0151]     After filtration, the lipoproteins in the filtered raw material are adsorbed onto an adsorbent. Any suitable adsorbent can be utilized. One example is silica-containing adsorbents. A silica adsorbent useful in this invention does not have a critical composition. Appropriate silica materials are the microfine silica available under the trademark Cabosil from Cabot Corporation and AEROSIL and SIPERNAT, such as the powdered silica SIPERNAT 50, manufactured by DeGussa and available from Cary Co. The silica is added to the liquid plasma or serum in an amount of about 1 to about 50 g/L, typically about 10 to about 20 g/L. The silica suspension in the liquid plasma or serum is then mixed for about 3 to about 4 hours.  
         [0152]     The adsorption can be carried out at a slightly acidic pH. Along these lines, the adsorption can be carried out at a pH of about 5 to about 8. Typically, the adsorption is carried out at a pH of about 5.8 to about 6.2. According to one example, the adsorption is carried out at a pH of about 6. Additionally, the adsorption can be carried out at a temperature of about 15° C. to about 30° C. for about 2 hours to about 24 hours. After adding the adsorbent(s), the solution can be mixed. According to one embodiment, the solution is mixed for about 30 to about 6 hours.  
         [0153]     After adsorption, the lipoprotein-adsorbent complex can be isolated and remaining portion of the raw material discarded. The isolation can be carried out as a simple phase separation utilizing a filter press.  
         [0154]     Subsequent to isolating the lipoprotein-adsorbent complex, occluded serum proteins can be removed from the lipoprotein-adsorbent complex. The removal can be carried out utilizing a high salt buffer wash. According to one example, this can be accomplished by washing the lipoprotein-adsorbent complex with an aqueous salt solution containing about 0.15 M sodium chloride. Other useful salts can include sodium acetate and/or sodium phosphate. The pH of the solution can also vary. Typically, the pH of the wash solution is about 6.9 to about 7.1. Similarly, the temperature that the wash is carried out at can vary. Typically, the temperature is about 2° C. to about 30° C. The salt solution is used in an amount about 120 liters for about each kilogram of the lipoprotein-adsorbent complex. Typically, the total volume of wash solution utilized could be about 12,000 liters to about 24,000 liters. According to one embodiment, two wash steps are carried out, each utilizing about 12,000 liters of wash solution. According to another embodiment, two wash steps could be carried out, each utilizing about 6,000 liters of solution. However, the volume could be more or less. The washing can be accomplished as a batch process or in a continuous washing process. According to one embodiment, the washing procedure is carried out at least two times as a batch process to remove occluded proteins. According to one particular embodiment, a first wash is carried out utilizing about 12,000 liters of a solution that contains about 8.3 to about 9.2 grams sodium chloride per liter and about 2.1 to about 2.9 grams sodium phosphate per liter at a pH of about 6.9 to about 7.1 and at a temperature of about 2° C. to about 30° C. This embodiment also can include carrying out a second washing step with about 12,000 liters of a solution that can include about 2.1 to about 2.9 grams sodium phosphate per liter at a pH of about 6.9 to about 7.1 at a temperature of about 2° C. to about 30° C. In embodiments that utilize a filter press to carry out the washing, the washing, whether a batch or continuous process, continues until reaching a target absorbance for the wash collection. According to one embodiment, the absorbance is less than about 0.1 at 280 nm. After washing the isolated the lipoprotein-adsorbent complex, the material utilized to remove the occluded proteins can be discarded.  
         [0155]     The purified lipoproteins can then be recovered from the adsorbent. The recovery can be carried out at an elevated pH. According to one embodiment, the recovery is carried out at a pH of about 10.5. According to another embodiment, the recovery is carried out by passing a high pH buffered solution through the lipoprotein-adsorbent complex until cholesterol is substantially removed from the adsorbent. After recovering the purified lipoproteins, the adsorbent is discarded.  
         [0156]     A solution containing the recovered lipoproteins can then be filtered. The filtration can be carried out utilizing one or more filtration steps. According to one embodiment, two filtration steps are utilized. A first filtration step utilizes filters having a nominal porosity of about 1 μ. A second filtration step utilizes membrane filters having a porosity of about 0.45 μ. In this and any of the filtration steps described herein, other filters can be utilized having different porosities as long as the porosity results in filtering particles of the desired size. Those of ordinary skill in the art would be able to determine suitable filter porosities without undue experimentation.  
         [0157]     After filtration, the recovered lipoproteins are exposed to an elevated pH. Exposing the recovered lipoproteins to the elevated pH appears to be significant in eliminating transmissible spongiform encephalopathy agent present in the recovered lipoproteins. Any suitable alkaline agent can be utilized to adjust the pH. According to one example, NaOH in a 1N solution was added to the recovered lipoproteins to achieve an elevated pH of between 10 to about 13. The exposure to the elevated pH can include any exposure from the briefest possible exposure up to many hours. Along these lines, the recovered lipoproteins can be exposed to an alkaline agent and the agent immediately neutralized. In such as case, the pH is not maintained at the elevated pH, but rather adjusted to the elevated value and then readjusted. The exposure in such a case can be as brief as practically possible. It appears, as discussed below, that even such a brief exposure can help to reduce TSE agent. Even though the pH exposure can be fleeting, the exposure is typically at least about 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12 hours. Typically, the pH is maintained at an elevated level for about 2 hours to about 12 hours. More typically, the pH is maintained at about 11 to about 13 for about 2 hours to about 8 hours. According to one embodiment, the solution is maintained at about pH of about 12 for about 8 hours. Longer periods of time can be utilized for the elevated pH exposure if deemed desirable and/or necessary. Those of ordinary skill in the art, once aware of the disclosure contained herein could determine acceptable pH levels and time periods without undue experimentation.  
         [0158]     Time and pH appear to be related in that a lower pH can be utilized if the time at the lower pH is longer as compared to higher pH. For example, a pH of about 10.5 can be utilized for a time longer than about 8 hours. A solution maintained at a high pH can be maintained at the lower pH for a comparatively shorter period of time.  
         [0159]     The elevated pH exposure step can be carried out at a temperature of about 18° C. to about 22° C. According to one particular embodiment, the elevated pH exposure step was carried out at a temperature of about 20° C. Temperature and time can also be related as pH and time. For example, a higher temperature can be utilized for a shorter period of time.  
         [0160]     After the elevated pH exposure step, the recovered lipoproteins can be subjected to additional steps to isolate them. The maintenance steps can include concentration/diafiltration by ultrafiltration. In this portion of the process, the concentrated cholesterol-rich solution can be dialyzed against an alkaline and/or a pH neutral material to further remove adsorbent that can include silica. Examples of materials that could be utilized in the dialysis include sodium carbonate and water. In order to improve the effectiveness of this dialysis step, it is desirable for the cholesterol-rich solution to be at a pH of about 7 to about 13, typically at a pH of about 8. The pH can be adjusted to this value by alkaline or acidic addition. This can take place just prior to the dialysis step, but typically, for operating convenience, the pH is adjusted to this value before the cholesterol-rich solution is subjected to an ultrafiltration concentration step.  
         [0161]     In the dialysis step, 8-12 volumes of deionized water can be utilized to remove the sodium carbonate. The resulting solution can then be concentrated by ultrafiltration prior to deionizing.  
         [0162]     The concentration/defiltration by ultrafiltration can be carried out until the solution including the recovered lipoproteins is concentrated by about 15 percent to about 50 percent. Typically, the solution is concentrated by about 20 percent to about 25 percent.  
         [0163]     The elevated pH exposure can be carried out at least partially during the concentration/diafiltration by ultrafiltration.  
         [0164]     Next, the concentrated solution is filtered. The filtration can be carried out utilizing one or more filtration steps. The filters utilized to carry out the filtration can have the capability to remove particles in the range of about 0.1 μ to about 1.0 μ. According to one embodiment, the solution is filtered sequentially through filters having porosities of about 0.65 μ and about 0.2 μ.  
         [0165]     Subsequent to filtration, the solution is subjected to a heat treatment. The heat treatment can include exposing the solution to elevated temperatures. The heat treatment can help to eliminate, reduce and/or inactivate viruses or prions that can be present in the solution.  
         [0166]     The heat treatment typically can include exposing the solution to a temperature of at least about 60° C. for a period of time of about at least about 10 hours. Typically, the solution is exposed to a temperature of about 60° C. to about 80° C. for a period of time of about 10 hours to about 14 hours. The solution can be exposed to about the same elevated temperature continuously. Alternatively, the solution can be exposed to different temperatures during the heat treatment. According to one embodiment, the heat treatment is carried out in three stages including a first stage at a temperature of about 80° C. for a time period of about 1 hour, a second stage at a temperature of about 65° C. for a time period of about 3 hours, and a third stage at a temperature of about 60° C. for a time period of about 10 hours. Any suitable time and temperature can be utilized to result in the desired effects on the solution. According to one embodiment, the time and temperature utilized in the heat treatment are sufficient to eliminate, reduce and/or inactivate viruses, according to generally accepted techniques for virus elimination, reduction and/or inactivation.  
         [0167]     After exposure to the heat treatment, the solution is subjected to filtration. The filtration can be carried out utilizing one or more filtration steps. The filters utilized to carry out the filtration can have the capability to remove particles in the range of about 0.1 μ to about 1.0 μ. According to one embodiment, four filtration steps are utilized to sequentially filter the solution with membrane filters of about 0.65 μ, about 0.45 μ, about 0.2 μ, and about 0.1 μ.  
         [0168]     Next, final cholesterol and pH adjustments can be made. While it is not necessary in the process for production of the cholesterol-rich fraction, it is convenient that the product have a pH adjusted to about 7.0 to about 8.4 so that it is generally compatible with media employed for cell culture.  
         [0169]     After adjustments to bring the cholesterol and pH to desired levels, the solution can be subjected to filtration. The filtration can be carried out utilizing one or more filtration steps. The filters utilized to carry out the filtration can have the capability to remove particles having a size in the range of about 0.1 μ to about 1.0 μ. According to one embodiment, four filtration steps are utilized to sequentially filter the solution with membrane filters of about 0.2 μ and about 0.1 μ. According to this embodiment, the solution is sequentially filtered through three filters having a porosity of about 0.1 μ. The solution typically is filtered into a sterile bulk container. Typically, the filtration is carried out in aseptic conditions.  
         [0170]     The solution can then be filtered again. The filtering can be carried out as the final product is introduced into a container for the final product, in other words, a container that the product will be made available to customers in. Therefore, the filtering is typically carried out as point-of-fill filtration. The filtration can be carried out utilizing one or more filtration steps. The filters utilized to carry out the filtration can have the capability to remove particles in the range of about 0.2 μ to about 1.0 μ. According to one embodiment, two filtration steps are utilized to sequentially filter the solution with membrane filters of about 0.2 μ. According to this embodiment, the solution is filtered sequentially through two 0.2 μ filters. After the final filtration, the product is ready to package for shipment. The process as described above produces a final yield of about 80 to about 120 milliliters from about I liter of starting material serum.  
         [0171]     This recovered purified lipoprotein/cholesterol complex is not pure cholesterol, but can be mixed with minor amounts of other materials, which passed through the production process. Along these lines, the complex typically is an aqueous mixture of cholesterol, phopholipids, and fatty acids. The resulting mixture has been found to be quite useful as a cell culture media supplement.  
       Example 2  
       [0000]     Use of EX-CYTE® to Reduce the Use of Serum  
         [0172]     Methods  
         [0173]     MK2.7 hybridoma cells were used. Seed inoculum was cultured in DME/F12 and FBS in spinners then adapted to less than 1% FBS by gradual reduction of FSB concentration. To begin the experiment, cells were washed in PBS and seeded at 1×10 2  cells/mL in each test condition. Batch cultures were sampled daily to monitor cell density and viability until culture viability was below 30%. Daily samples of culture supernatant were taken and processed to measure antibody production by ELISA.  
         [0174]     Results  
         [0175]     A combination of 0.5% EX-CYTE® and 2% FBS allowed for higher cell density and prolonged viability throughout the life of the culture as compared with 10% FBS ( FIG. 1 ). The accumulative antibody level in the 0.5% EX-CYTE® and 2% FBS condition was more than double that of the 10% FBS culture on day 7 ( FIG. 2 ). As a result, 0.5% EX-CYTE® effectively allowed the reduction of FBS from 10% to 2%.  
       Example 3  
       [0000]     Use of EX-CYTE® to Replace Serum  
         [0176]     Methods  
         [0177]     MK2.7 hybridoma cells were used. Seed inoculum was cultured in DMEM and FBS in spinners then adapted to less than 1% FBS by gradual reduction of FBS concentration. To begin the experiment, cells were washed in PBS and seeded at 1×10 5  cells/mL in each media condition. The test condition consisted of 0.75% EX-CYTE® 0.4% BSA, 6.7 ug/L sodium selenite. 10 mg/L insulin and 5.5 mg/L transferrin. (BSA (Serologicals Catalogue Number 81-068). Insulin (Serologicals Catalogue Number 4506), Transferrin (Serologicals Catalogue Number 4465)). Batch cultures were sampled daily to monitor cell density and viability until culture viability was below 10%. Daily samples of culture supernatant were taken to measure antibody production by ELISA.  
         [0178]     Results  
         [0179]     A combination of 0.75% EX-CYTE® and 0.4% BSA in DMEM constituted a complete serum-free media formulation. The temporary drop in culture viability in the test condition on days two and three was likely due to culture adaptation from low serum condition to serum-free condition ( FIG. 3 ).  FIG. 4  shows that comparable levels of production are achieved despite an overall drop in cell mass in the test condition, which is consistent with a shift in metabolic effort from growth in protein production. Productivity data of 10% FBS after day 8 was now shown because viability had decreased to less than 10%.  
       Example 4  
       [0000]     Use of EX-CYTE® to Boost Performance of Serum-Free Media  
         [0180]     Methods  
         [0181]     MK2.7 hybridoma cells were used. For each experiment, seed inoculum was adapted to each SFM according to the media manufacturers&#39; recommendations. To begin the experiment, SFM adapted culture was seeded at 1×10 5  cells/mL in each condition, Batch cultures were sampled daily to monitor cell density and viability until culture viability was below 10%. Daily samples of culture supernatant were taken and processed to measure antibody production by ELISA.  
         [0182]     Results  
         [0183]     As shown in  FIG. 5 , the addition of 0.2% BSA and 0.5% EX-CYTE® to EX-CELL™ 620 Serum-free Media doubled the total cell mass over the life of the culture. The addition of EX-CYTE® and BSA significantly prolonged the life of the culture from eight days to greater than twelve days with increased viability. The peak IgG1 productivity in EX-CELL™ 620 supplemented with EX-CYTE® and BSA was 25% higher on day 10 compared with the EX-CELL™ 620 alone as shown in  FIG. 6 .  
         [0184]     Similar effects were observed in Hybridoma Medium, Animal Component-free when supplemented with 0.5% EX-CYTE® AND 0.2% BSA. The life of the culture in Hybridoma Medium, Animal Component-free was prolonged from 7 days to greater than 12 days by the addition of EX-CYTE® and BSA ( FIG. 7 ). The peak accumulated antibody level on day 12 in Hybridoma Medium, Animal Component-free was increased by 38% by the addition of EX-CYTE® and BSA ( FIG. 8 ).  
       Example 5  
       [0000]     Use of XCF-1 Formulation to Boost Growth and Performance of Hybridoma Cell Lines  
         [0185]     Methods  
         [0186]     Six hybridoma cell lines were used, including MK2.7.4 hybridoma cells and 5C8 hybridoma cells. A composition termed XCF-1 was prepared containing DMEM (Gibco #11960-051), 4 mM glutamine (Gibco #25030-081), 2 % EX-CYTE ® (Serologicals Inc. # 81-129-081; Lot 420), 0.5% BSA (Serologicals Inc. #81-068; Lot 745), 10 mg/L human recombinant insulin (Serologicals Inc. #2002712), 1 mg/L human holo-transferrin (Serologicals, Inc. #4455-80), and 10 μM ethanolamine (Sigma # E-0135). Daily samples of culture supernatant were taken and processed to measure protein production by ELISA. Cell density was also measured.  
         [0187]     Results  
         [0188]     XFC-1 achieved cell density criteria in 5 out of 6 cell lines. XCF-1 also achieved protein production criteria in 4 of out of 6 cell lines. Data from these experiments in summarized below in Table 3. Data is normalized with 10% FBS representing 100%.  
                                                   TABLE 3                           XCF-1 Performance in Hybridoma Cells                    Ave. Cell Density   Ave. Production                            Hybridoma A   1.15   0.923           Hybridoma B   1.28   0.9065           Hybridoma C   0.88   0.555           Hybridoma D   0.85   0.855           5C8.33 Hybridoma   0.989   1.15           MK2.7.4   0.979   0.986                      
 
         [0189]     Data for particular hybridoma cell lines is also given. As shown in Table 4, the addition of XCF-1 achieved cell density criteria and protein production criteria in MK2.7.4 cells.  
                                               TABLE 4                           XCF-1 Performance in MK2.7.4 Cells                Cell Density   Protein Production                            Experiment # 1   0.782   0.759           Experiment # 2   1.22   1.24           Experiment # 3   0.935   0.959           Average:   0.979   0.986           Standard Deviation   +/−0.222   +/−0.242                      
 
         [0190]     As shown in Figure Table 5, the addition of XCF-1 achieved cell density criteria and protein production criteria in 5C8 cells.  
                                               TABLE 5                           XCF-1 Performance for 5C8 hybridoma cells                Cell Density   Protein Production                            Experiment # 1   0.961   1.14           Experiment # 2   1.078   1.47           Experiment # 3   0.919   1.17           Experiment # 4   0.999   0.80           Average:   0.989   1.15           Standard Deviation   +/−0.068   +/−0.272                      
 
       Example 6  
       [0000]     Use of XCF-2 Formulation to Boost Growth and Performance of Cancer Cell Lines  
         [0191]     Methods  
         [0192]     Three cancer cell lines were used, including K562, Jurkat and EL-4. A composition termed XCF-2 was prepared containing RPMI 1640 (Sigma # R5886), 4 mM glutamine (Gibco #25030-081), 0.1% EX-CYTE® (Serologicals Inc. # 81-129-2; Lot 420), 1% (Serologicals Inc. # 81-068), 10 mg/L human recombinant insulin (Serologicals #2002712), 1 mg/L human holo-transferrin (Serologicals Inc. # 4455-80); 0.1% peptone (such as Primatone RL (Quest)), 12.5 micrograms/ml fetuin (Pedersen) (Serologicals Inc. # 4570-01), and 5 micromolar vitamin E (Sigma # T3251-5G). Cell density was measured.  
         [0193]     Results  
         [0194]     XCF-2 achieved cell density criteria in 3 out of 3 cell lines. Data from one cell line, 562, is summarized in Table 5. Data is normalized with 10% FBS representing 100%.  
                                       TABLE 6                           XCF-2 Performance in K562 Cell Line                Cell Density                            Experiment # 1   0.939           Experiment # 2   0.796           Experiment # 3   0.850           Experiment # 4   0.903           Experiment # 5   0.897           Average   0.877           Standard Deviation   +/−0.055                      
 
         [0195]     Many modifications and other embodiments of the invention come to mind to one skilled in the art to which this invention pertains having the benefit of the teachings presented in the foregoing descriptions and the associated drawings. Therefore, it is to be understood that the invention is not to be limited to the specific embodiments disclosed and that modifications and other embodiments are intended to be included within the scope of the appended claims. Although specific terms are employed herein, they are used in a generic and descriptive sense only and not for purposes of limitation.  
       Example 7  
       [0196]                                  TABLE 7                           Representative XCF2 Formulation            XCF2 Constituents   Source   Catalog Number               EXCYTE (0.1%)   Serologicals   81-129-2       BSA (1%)   Serologicals   81-068 (lot 745)       rHuman Insulin (10 mg/L)   Serologicals   2002712       Hu Holo-Transferin (1 mg/L)   Serologicals   4455-80       Pedersen Fetuin (12.5 mg/ml)   Serologicals   4570-01       Primatone RL (0.1%) or Soy   Quest       Peptone       Sodium Selenite (25 nM)   Sigma   S-5261       Vitamin E (5 mM)   Sigma   T3251-5G                    
 XCF2 Cell Lines 
 
         [0197]     Numerous representative cell lines were been tested for growth with the XCF2 formulation in plate culture experiments. The following cell lines were used: K562—human hematopoietic, EL-4—mouse T-Lymphocyte, HL-60—human promyelocyte, Daudi—human B lymphoblast, HeLa—human cervical adenocarcinoma, THP-1—human monocyte and Jurkat—human T-Lymphocyte. All cell lines were adapted to 0.5% FBS, 1× Gibco ITS in RPMI with 4 mM Glutamine prior to testing. Some cell lines (Daudi and THP-1) were maintained and tested in XCF2 with the ATCC recommended additive of sodium pyruvate.  
         [0000]     Experimental Design for Plate Studies  
         [0198]     Cells were harvested from expansion growth in low serum adapted culture, washed and then plated at a density of 100,000 cells per well in each treatment. For all plate cultures, 24 well non-tissue culture treated plates were used. Cell viability and density were measured by propidium iodide incorporation and flow cytometry.  
         [0000]     Surface Marker Expression Analysis by Flow Cytometry  
         [0199]     K562 cells were used as a representative cell type for cancer/suspension cell lines. Investigations were designed to test expression of cell surface markers that have been identified as useful in research investigations. Two experimental designs were utilized to assess the expression of cell surface markers of b1-integrin (CD29), Fc receptor (CD32) and sialoglycoprotein (CD43) on K562 cells. In one design, expression after four days in culture in either low serum conditions (0.5% FBS), normal serum conditions (10% FBS) or XCF2 was measured. In the second experimental design, cells can be continually passaged in either XCF2 or 10% FBS and expression can be compared over a two week time period.  
         [0200]     Results  
         [0201]     EX-CYTE and BSA Optimization:  
         [0202]     Initial studies were conducted with K562 cells in RPMI, Glutamine, and varying concentrations of EX-CYTE and BSA.  FIG. 9  shows the gross range finding results and  FIG. 10  demonstrates a refined range find that resulted in the current formulation of 0.1% EX-CYTE and 1% BSA to be selected.  
         [0000]     Human Recombinant Insulin and Human Holo-Transferrin  
         [0203]     Following the initial set of experiments, the concentration of Insulin and human holo-transferrin most beneficial was identified. From experiments conducted, 10 mg/L of Insulin and 1 mg/L of human holo-transferrin was determined to be an optimal amount.  
         [0000]     Addition of Sodium Selenite and Ethanolamine  
         [0204]     Sodium selenite was included at the level of 25 nM. Ethanolamine showed no effect and was excluded from this particular formulation.  
         [0000]     Experimental Studies to Determine the Usefulness of Primatone, Fetuin and Vitamin E  
         [0205]     Through a series of experiments including one to determine optimal concentrations and a second experiment investigating omission of these three components it was determined that Primatone at a concentration of 0.1%, Pedersen Fetuin at 12.5 mg/ml and Vitamin E at 5 mM would promote optimal cell density and growth. In the second set of experiments, omission studies showed that Primatone was a major contributing factor to growth promotion and viability. Pedersen Fetuin was important and contributed a promotion effect that boosted cell density approximately 10-20%.  
         [0000]     Evaluation of XCF2 Performance When Primatone Rl is Substituted with Soy Peptone  
         [0206]     Soy Peptone can replace Primatone RL thus reducing the number of bovine derived constituents in the XCF2 formulation. In a growth promotion assay, K562 cells grown in XCF2 in RPMI containing either Primatone RL (0.1%) or Soy Peptone (0.1%) achieved equivalent cell density and viability. Performance from two replicate experiments are shown in  FIG. 11 .  
         [0000]     Repeat Evaluations of XCF2 with K562 Cells  
         [0207]     To ensure peak performance of the formulation, multiple replicates were tested to compare the final XCF2 formulation and the benchmark. In 4 of 5 experiments the criteria of 85% was met for cell density and viability as referenced to the 10% FBS benchmark. Cell density data for this set of experiments is shown in  FIG. 12 .  
         [0000]     Evaluation of Xcf2 with Different Cell Lines  
         [0208]     XCF2 has been tested in seven different cancer/suspension cell lines and the results from these tests have been presented here in tabular format. The cell lines include 6 human cell lines (K562, HL-60, Daudi, HeLa, THP-1 and Jurkat) and one mouse cell line (EL-4). Five of the seven cell lines matched the necessary performance criteria relative to the benchmark of 10% FBS. The minimal acceptable criteria was 0.85 for cell density and 0.85 for cell viability. Daudi, EL4, HeLa, HL-60 and K562 cells all performed at or above the minimal performance criteria. While Jurkat and THP-1 performance was above criteria for viability, it was below criteria for cell density. THP-1 cell density was 82% and Jurkat cell density was 73% of that achieved with 10% serum.  
         [0209]     Cell morphology for six of the seven cell lines was as expected, with the one exception being HeLa cells. Although HeLa cell proliferation was approximately 2× greater in XCF2 than in 10% FBS, the cells were not adherent.  
                                                           TABLE 8                           Summary of results on XCF2 with different cancer cell lines            Cell   Average Cell   Average Cell   Experimental           Type   Density   Viability   Duration   #Replicates                    Daudi   0.91   0.95   7 Days   2       EL4   0.86   0.97   4 Days   2       HeLa   2.02   1.22   10 Days    2       HL-60   1.26   1.10   14 Days    1       K562   0.88   0.95   7 Days   5       Jurkat   0.73   0.96   7 Days   2       THP-1   0.82   0.97   7 Days   2                  
 
 XCF2 Performance in Basal Media 
 
         [0210]     Two basal media (DMEM and RPMI) were selected for comparison of XCF2 performance. XCF2 performed well in both media.  
         [0000]     K562 Growth in Continuous Culture and in Direct Adaptation from LN2 Storage into XCF2  
         [0211]     Cells removed from LN2 storage and seeded directly into XCF2 achieved a doubling time of 20 hours within 10 days. The standard doubling time for K562 cells as reported in the literature is ˜20-21 hours. Using the K562 cells, the data demonstrates that the adaptability and doubling time for cells grown in XCF2 can achieve normal rates within the prescribed 14 day time-frame.  
         [0000]     Surface Marker Expression on K562 Cells Grown in XCF2  
         [0212]     All three surface markers were expressed at comparable levels on K562 cells grown in XCF2 or 10% FBS in RPMI and DMEM. The graph in  FIG. 13  shows the expression of CD32 minus the isotype control. Cells were grown in either 10% FBS or XCF2 for four days and then the surface marker expression was measured by flow cytometry.  
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             TABLE 1                           SERUM-FREE CULTURE MEDIA                                                                  PRODUCT   COMPOSITION   APPLICATIONS   CELLS                        ATHENA ENVIRONMENTAL SCIENCES       www.athenaes.com       UK suppliers Stratech at www.stratech.co.uk            1   BRFF-BMZERO™   Complete serum-free medium   Designed for growing bitten   Human breast cells                   breast cells. Used for                   establishing new epithelial cell                   lines from explants of human                   breast tissue and growing                   immortalized normal breast                   cell lines.       2   BRFF-EPM2™   Complete serum-free medium   Designed to grow human   Human epidermal cells                   epidermal-like cells   Human oesophageal epithelial cells                   Originally optimised for   Human skin explants                   human oesophageal epithelial   Human cancer cell lines                   cells. Explant cultures of                   human skin in BRFF-EPM2                   yield outgrowths of epithelial                   cells. Also useful for culturing                   some human cancer cell lines.       3   BRFF-HPC1™   Complete serum-free medium.   Designed for establishing new   Human prostate tissue               Contains dihydrotesterone.   cell lines from human prostate                   tissue. Epithelial cell lines,                   from both benign prostatic                   hyperplasia and prostatic                   carcinoma have been                   established and maintained in                   this medium.       4   BRFF-P4-8F™   Complete serum-free medium.   Designed to grow   Normal prostatic cell line 267-B1                   immortalized normal prostatic   Prostatic cancer cell lines, e.g.                   cell line 267-B1. Also supports   PC-3                   the growth of certain                   established human prostatic                   cancer cell lines such as PC-3.       5   Serum-free Media Screening Kit   Kit contains a 100 ml sample of   Screening kit is intended for               each of five serum-free media,   researchers seeking to identify               ready for use. These are BRFF-   the most appropriate serum-               BMZERO™, BRFF-EMP2™,   free medium for a specific cell               BRFF-P4-8F™, DMEM/F12,   type.               and IMDM.            ATLANTA BIOLOGICALS       www.atlantabio.com                                                                                                                                                                                                                                                                                                                                                                                     BD BIOSCIENCES/BECTON, DICKINSON &amp; COMPANY       www.bdbiosciences.com                                                                                                                                                                                                              9   BD Cell™ MAb Media, Serum   Complete serum-free HEPES   Supports a wide variety of   Various myeloma fusion partners           Free   based medium. Contains   myeloma fusion partners and   and hybridomas including Sp2/0,               bovine serum albumin, L-   hybridomas. Designed to   NS-1, P3X63Ag9, and FOX-NY.               Glutamine and phenol red.   enhance monoclonal antibody   as well as secreting cell lines               Without pluronic acid or other   production.   such as CHO.               surfactants.                                                                                                                                                                                 11   BD Hepato-STIM™   Serum-free, fully defined       Hepatocytes               culture medium.            BIOCHROM       www.biochrom.de       Products also supplied by Autogen-Bioclear www.autogen-bioclear.com                                    12   HybridomaMed DIF 1000   Serum-free medium based on a   Developed for growth of   Hybridoma               1:1 mixture of Iscove&#39;s   hybridoma, but also suitable   YAC-1 (mouse T-cell Lymphoma)               medium and Ham&#39;s P12,   for other cell lines.   HeLa (human epitheloid cervical               supplemented with transferrin,       carcinoma) BJA-B (human EBV-               insulin and a BSA/oleic acid       negative carcinoma)               complex.       BHK-21 (Syrian hamster kidney)                       L-psv 129 mouse L-fibroblast       13   MCDB 153   Serum-free basal medium,   Supports the growth of human   Keralinocytes               requires supplementation with   keratinocytes.               EGF, insulin, hydrocortisone,               ethanolamine and               phosphethanolamine.       14   MCDB 153 complete medium   Serum-free ready to use   Supports the growth of human   Keratinocytes               medium   keratinocytes.       15   Octomed   Serum-free and protein-free   Designed for growth of   CHO cells               medium.   Chinese Hamster Ovary cells.       16   PFEK-1   Serum-free and protein-free   For proliferation of VERO   VERO cells               medium.   cells, for propagation of                   human pathogenic viruses                   (Coxsackie B4, herpes simplex                   type 1 and 2, measles, polio                   type 1-3).       17   Sebomed Complete medium   Serum-free. Modified   Optimised for growth of   Human sebaceous gland cell line               DMEM/Ham&#39;s F-12 (1:1)   human sebaceous gland cell   SZ9S               Supplemented with EGF rh,   line SZ95 in vitro               BPE, BSA, linoleic acid.               Formulation available on               request.       18   TNB 100 Medium   Serum-free medium   Optimised for cultivation of   Neuronal cell lines (mouse, chick                   neuroblastoma x glioma cells.   and rat). Human cytotoxic T-cells.                   Also useful for study of non-                   neuronal cells such as human                   cytotoxic T-cells.            BIOLOGICAL INDUSTRIES       www.bioind.com                                    19   BIOTARGET-1   Serum-free medium   For use with mononuclear   Mononuclear cells (lymphocytes                   cells from peripheral blood.   and monocytes)                   Applications include                   activation of mononuclear                   cells with mitogens; activation                   with lymphoid cells;                   production of IL-2 and IL-3;                   generation of LAX and TIL;                   generation of natural killer                   cells and cytotoxic T cells.                   activation of macrophages;                   proliferation of HIV,                   retroviruses in T cells for                   vaccine development.       20   BIO-MPM-1   Serum-free media. Contains   Multi-purpose media for   Various               insulin   adherent cells               Without albumin. growth               factors, or hormones (other               than insulin)       21   BIOCHO-1 SFM Base   Serum-free base media,   BIOCHO-1 for adherent CHO   CHO cells of various kinds           &amp; BIOCHO-2 SFM Base   contains amino acids, vitamins.   cells and BIOCHO-2 for           BIOGRO-CHO SFM   salts, lipids, trace elements.   suspension cultures.           Supplement   Supplement for above base.               containing proteins.               The complete medium does not               contain albumin, growth               factors, or hormones (other               than insulin)       22   BIOINSECT-1   Serum-free medium   Culture of lepidopteran insect   SF-9                   cells       23   Serum-free Cell Freezing   Serum-free medium for   Cryopreservation medium   3T3, BGM, Vero, Hep2, BSC1           Medium   freezing cells. Contains               methylcellulose and DMSO            BIO MEDIA       www.biomediaworld.com                                                                                                                                                                                                                   CAMBREX       www.cambrex.net                                    25   BioPro 1   Low protein, serum-free liquid   Supports CHO cell lines   CHO               medium, without L-glutamine               and without glucose.                                                                                                                                                                                 27   UltraCHO™   Serum-free modified DMEM:   Optimised to support the   CHO lines               F12 base, supplemented with   growth of transfected and non-   HeLa cells (suspension or attached)               insulin, transferring, and   transfected CHO cells   Human leukaemia cell lines               proprietary purified proteins.               Contains L-glutamine.       28   PC-1™   Complete liquid media system   All purpose medium for   Cell lines: HeLa, MRC-5,               with frozen supplement. Low   culture of primary cells and   BHK-21, WI-38, NRK, 3T3,               protein and serum-free,   anchorage dependent cell lines   CHO-K1, HTB-72, HRB-4, WISH,               Modified DMEM/F12 base,       VERO, MDCK, STO, Hep-2,               contains HEPES buffering       SIRC, C6, T9, ARL6T.               system, insulin, transferrin and       Primary cells:               fatty acids.       Human neuroblastoma, foreskin                       fibroblast, bladder carcinoma, renal                       papillary collecting tubule, colon                       epithelium, colon carcinoma. Rat                       dermal fibroblast, mammary                       carcinoma, neonatal normal cardiac                       muscle, astrocytes, thyroid                       epithelium. Baboon spinal ganglia,                       swine testes cell, bovine kidney.       29   UltraCULTURE™   Complete serum-free media   All purpose medium to   Human cells: HEL, N-10, HeLa,               consisting of a DMEM: F12   support he growth of a wide   HuL-1,2, HuK-1, HuS-1 AT, HEC,               base, supplemented with   variety of both adherent and   HuL-1,2, K-562, HNK, HTC29,               bovine insulin, bovine   non-adherent cell lines. Has   TT, MB231, U138.               transferrin and purified mixture   been used to grow cells of   Mouse cells: FM3A, NS-1, L,               of bovine serum proteins   primary origin and established   P388D1, P815, T3, B32.               including albumen   cell lines. Used to grow cells   Rat cells: RPL-1, RSP-2, RLG-1,                   of lymphoid origin, including   Lynn-1, RCR-1, 235-1, MMQ, GC                   monocyte and macrophage cell   GH3, CA77, Ras-1.                   lines; epithelial and   Monkey cells: JTC-12, COS1, and                   fibroblastic cells; fusion of   CO57.                   cells during hybridoma                   formation, and generation of                   viral particles for use in                   vaccine production.       30   CellGro® SCGM   Serum-free medium containing   To sustain the growth of   Human hematopoietic progenitor               only human derived or   human hematopoietic   cells               recombinant human proteins   peripheral blood progenitor               Without cytokines. or other   cells for gene therapy research.               growth factors.       31   UltraDOMA™   Serum-free medium composed   Supports the growth of   Murine hybridomas               of RPMI-1640 base,   murine, human and chirneric   NS-1 derived myelomas               supplemented with bovine   hybridomas for monoclonal   SP-2 derived myelomas               insulin, bovine transferring and   antibody production in batch               bovine albumen,   culture and in hollow fibre               Without L-glutamine.   bioreactors.       32   UltraDOMA-PF™   Serum-free and protein-free   Supports the growth of   Murine hybridomas               medium. Contains L-glutamine   murine, human and chimeric   NS-1 derived myclomas                   hybridomas for monoclonal   SP-2 derived myclomas                   antibody production. Designed   Rat hybridoma                   for lab or industrial scale use.   Some transfected CHO cell lines                       Human lymphoid origin cells                       Murine lymphoid origin cells                                                                                                                                                                                 34   Nephros™ -LP   Serum-free medium.   Optimized to support growth   Feline kidney cells (CREK)               supplemented with growth   of kidney cells.   Porcine kidney cells (PK-15)               factors and       Buffalo African Green Monkey                       kidney cells (BGMK)       35   HL-1   Serum-free and chemically   Supports growth of many   Human cell lines U937, Raji,               defined medium. Ingredients   hybridomas and other cell   MCF-7 (NIH), MCF-7 (MCF),               include water, a modified   types of lymphoid origin.   NIH ZR-75, COLO 302 HSR, J82,               DMEM/F12 base, HEPES       SW1738 SW780, CCL 119, CCL               buffer, known amounts of       213, C91/PL, astrocytoma,               insulin, transferrin,       hepatoma, MOLT-3,               testosterone, sodium selenite,       MOLT-4, NAMALWA, THP-1               ethanolamine, saturated and       Murine cell lines:               unsaturated fatty acids and       BB88, P815, P388D1, WeNi3,               proprietary stabilizing proteins.       JLS-V5, GCL2, 70Z-3, 70Z/3.12,               Without bovine serum albumen       S49 and variants, RAW309F1.1,               or other undefined protein       WeHi7, L5178Y, 1-10, EL4, RL1,               mixtures.       BW5147.3, LBRM-3 3, Friend                       leukaemia, C57BL6.                       Other species cell lines:                       CHOK, VERO, MDMK.                       Various Hybridomas                       Primary cells:                       Human peripheral blood T                       lymphocytes,                       Human blood monocytes                       Human fetal adrenal, Mink                       lymphocytes.                                                                                                                                                                                 37   UltraMDCK™   Serum-free and low protein   Designed to support growth of   Madin-Darby Canine Kidney cells               basal medium supplemented   MDCK cells at low and high   (MDCK)               with only two proteins: bovine   plating densities. Suitable for               insulin and bovine transferrin.   large scale bioprocessing and                   for in vitro diagnostic use.       38   Insect-XPRESS™   Serum-free and protein-free   Designed to support the   Sf-9 and Sf-21 cells, attached               medium. Contains L-Glutamine   growth of invertebrate cell   and suspension culture                   lines derived from Fall Army                   worm, Spodoptera frugiperda                   (Sf), under attachment                   dependent or attachment                   independent conditions.                   Supports production of                   recombinant proteins by cells                   infected with viral vectors                   such as BEVS.            CASCADE BIOLOGICS       www.cascadebio.com       UK suppliers: Patricell Ltd at www.patricell.com                                    39   Epilife Medium   Serum-free HEPES based   Primary isolation and long-   Human corneal epithelial cells               medium. Requires supplementation   tem culture of normal human   Human epidermal keratinocytes.               with one of the following:   corneal epithelial cells and               Epilife Defined Growth   human epidermal keratinocytes.               Supplement-contains BSA,   For lab research only.               bovine transferrin,               hydrocortisone, rh insulin-like               growth factors.               Human Corneal Growth               Supplemen-contains bovine               pituitary extract, bovine               insulin, hydrocortisone, bovine               transferrin, and mouse               epidermal growth factor.               Human Keratinocyte Growth               factor,-bovine pituitary               extract, bovine insulin,               hydrocortisone, bovine               transferrin, and human EGF.            GIBCO™ INVITROGEN CORPORATION       www.invitrogen.com                                                                                                                                                                                                                                                                                                                                                                                42   AIM V       Ex vivo activation of cytotoxic   Lymphocytes                   lymphocytes with IL-2                   supplementation Growth of                   tumour infiltration (TIL cells)                   or cytotoxic T-cells. HIV virus                   production.       43   CHO-S-SFM II   Low protein (&gt;75 μg/ml)   Growth and production of   Chinese Hamster Ovary (CHO)               serum-free medium   recombinant proteins in   suspension cultures                   suspension culture.       44   CHO III PFM   Protein-free medium   Growth and production of   CHO cells                   recombinant proteins in   suspension cultures                   suspension culture.       45   CHO III A PFM   Protein-free medium   Growth and production of   CHO cells                   recombinant proteins in                   adherent culture.       46   CHO-A-SFM   Low protein (&lt;250 μg/ml) and   Growth and production of   CHO cells               serum-free   recombinant proteins in                   adherent culture.                                                                                                                                                                                 48   Drosophila-SFM   Protein-free and serum-free   Growth and maintenance of   Drosophila melanogaster cells,               medium   adherent or suspension culture.   (D.Mel2, Schoader S2 cells)       49   Express Five SFM   Protein-free and serum-free   Growth and maintenance of   BT1.TN-5B1-4 insect cells.                   BT1-TN-5B1-4 insect cells                   used for the baculovirus                   expression vector system                   (BEVS) for adherent or                   suspension cultures. Large-                   scale production of                   recombinant protein expressed                   by BEVS.       50   Endothelial-SFM       Growth and maintenance of   Bovine, ovine and porcine,                   endothelial cells for studying   vascular endothelial cells                   cell-cell interactions, injury                   analysis and atherosclerosis.       51   Human Endothelial-STM   Serum-free, supplied with   Growth and maintenance of   Primary and secondary human               growth factors and attachment   human endothelial cells to   umbilical venous (HUVEC),               factors (bFGF, EGF,   study signal transduction,   dermal microvascular and arterial               fibronectin)   cytokine production, and cell   endothelial cells                   adhesion.       52   HepatoZYME-SFM   Serum-free   Maintenance of primary   Primasy human, rat and monkey                   hepatocyte cells (cytochromes   hepatocytes                   P450 induction maintained &gt;9                   days)       53   Hybridoma-SFM   Serum-free, low protein   Hybridoma growth and   Mouse, human and rat hybridomas               (&lt;20 μg/ml as insulin and   monoclonal antibody   (AE-1, L5.1, L243, Sp2/0)               transferrin) medium. Contains   production.               Phenol red and surfactant.               Supplementation with a               lipoprotein preparation required               for cholesterol dependent cell               lines (e.g. NSO and derivatives.                                                                                                                                                                                 55   PFHM-II   Protein-free, serum-free,   Growth and production   Mouse, human and rat hybridomas               medium. Contains Phenol red   medium for hybridoma cells.               and inorganic iron carrier.       56   Defined Keratinocyte-SFM   Low protein serum-free   Growth of primnazy and   Human keratinocytes               medium, plus insulin,   secondary human               epidermal growth factor, and   keratinocytes.               fibroblast growth factor.       57   Keratinocyte-SFM   Basal serum-free medium, plus   Studies with dermal   Human keratinocytes               bovine pituitary extract, human   substitutes, in vitro toxicology               recombinant epidermal growth   and gene therapy. Has been               factor.   used to cultivate cervical                   epithelial cells, and to study                   human papillomavirus DNA                   transfected cells. May be                   suitable for cultivating other                   epithelial cells such as human                   bronchial epithelial cells.       58   Macrophage-SFM       Growth and maintenance of   Macrophages and monocytes                   macrophages sad monocytes                   (addition of GM-CSF may be                   necessary). Demonstration of                   macrophage phagocytosis.                   Activation of cells to kill                   tumour cells with gamma                   interferon or                   lipopolysaccharide                   supplementation.       59   Neurobasal™ Medium   Basal medium lacking   Long-term growth of foetal   Foetal neurons               excitatory amino acids used in   neurons.               conjunction with supplements               below to make a complete               serum-free medium.       60   Neurobasal™ A Medium   Basal medium lacking   Long-term growth of postnatal   Adult and postnatal neurons               excitatory amino acids used in   and adult neurons   (&gt;1 week old)               conjunction with supplements               below to make a complete               serum-free medium.       61   Neurobasal Supplements   Supplement B27. A serum   Low-density plating and long-   Primary rat embryonic               substitute   term viability and growth of   hippocampal neurons Primary rat                   hippocampal and other CNS   neurons from striatum, substantia                   proliferation.   nigra, septum and cortex               Supplement B27 AO [B27   Studies of oxidative damage               without any cortex   and rescue, apoptosia, or age-               antioxidants]   related neurodegenerative                   diseases where free radical                   damage to neurons occurs               Supplement B-27 Without   Supports growth of CNS   Primary rat embryonic               Vitamin A   progenitor or stem cells/   hippocampal neurons, tumour cell                       lines of neural origin (PC12,                       B104, NIE-115, NS20).               Supplement N2   Maintenance and growth of rat   Primary glial cells, tumour cell               Chemically defined   embryonic hippocampal   lines of glial origin (U-251. Mgsp,               supplement, containing insulin   neurons. Growth and   C62BD, RN-22), astrocytes,               (bovine), human transferring   maintenance of tumour cell   microglia, oligodendrocytes.               (holo), progesterone,   lines of neural origin.               putrescine, and selenite.               Supplement G5   Growth and maintenance of               Chemically defined additive   glial cells.               containing insulin, human               transferrin, selenite, biotin,               hydrocortisone, FGF and EGF.       62   HIBERNATE-E   With B27 supplement   Short-term maintenance (2   Embryonic neural tissue                   days) of neurons in ambient                   CO 2  if precultured in                   Neurobasal medium with B27.                   Long-term storage (1 month)                   at 4° C. of prenatal brain tissue.       63   HIBERNATE-A   With B27 supplement   Short-term culture (2 days) of   Postnatal and adult neural tissue                   neurons in ambient CO2 if                   precultured with Neurobasal                   Medium with B27. Storage of                   postnatal and adult brain                   tissue                                                                                                                                                                                 65   OPTI-MEM® I reduced Serum   Reduced serum medium, a   Most cells routinely cultured   Various           media   modification of Eagle&#39;s   in serum-supplemented               Minimum essential medium   medium may be transferred               buffered with HEPES and   directly into OPTI-MEM® I               sodium bicarbonate,   with a minimum of 50%               supplemented with   reduction in serum. Very low               hypoxanthine, rhymidine   serum supplement achieved               sodium pyruvate, L-glutamine,   with myelomas and derived               trace elements and growth   hybridomas, fibroblasts,               factors. Phenol red reduced to   epithelial cells or normal and               1.1 mg/L   tumour origins.       66   Sf-900 II SFM   Protein-free and serum-free   Growth and maintenance of   Sf9, Sf21, TN368 cells                    cells used for baculovirus   (Spodoptera frugiperda)                   expression vector system                   (BEVS) for adherent or                   suspension culture. Large-                   scale production of                   recombinant protein expressed                   by BEVS.       67   STEMPRO-34® SFM   Serum-free medium. Requires   Supports growth of human   Human hematopoietic progenitor               addition of   hematopoietic progenitor cells   cells (CD34+) from bone marrow,               L-Glutamine, and Stempro   (CD34+). Optimized using   peripheral blood or neonatal cord               supplement at time of use.   freshly isolated CD34+ bone   blood               Extended cell support requires   marrow cells from normal               addition of cytokines and   donors.               growth factors.                                                                                                                                                                                      HYCLONE       www.hyclone.com                                    69   SFM4Mab™ -Utility   Serum-free medium, contains   Designed to support growth of   Multiple hybridoma cell types               cholesterol and L-Glutamine.   multiple hybridoma cell types.               Without Phenol Red or   For production of monoclonal               Pluronic F-68   antibodies for academic and                   industrial research, genomics                   and proteomics, in vitro                   diagnosis, drug target                   screening and validation, and                   manufacturing of preclinical                   lots.       70   SFM4Mab™   Serum-free medium, contains   Designed to increase process   Engineered bybeidoam and               synthetic cholesterol and   yields for the industrialised   meombinam myelonm cell lines.               Pluronic F-68. Without Phenol   manufacture of human and               Red. Available with or without   humanized recombinant               L-Glutamine.   antibodies for therapeutic use                   in a variety of engineered                   hybridoma and recombinant                   myeloma cell lines.       71   SFM4CHO™   Serum-free and protein-free   Designed to increase process   CHO               medium, with only minimal   yields for the industrial               animal derived components   manufacturing of recombinant               (cholesterol and cod liver oil),   proteins in a variety of CHO               and no components of bovine   cells. Designed to support the               origin. Contains Pluronic P-68,   DHFR election/amplification               and 2.2 g/l NaHCO 3 . Available   system, and to support the GS               with or without L-Glutamine.   gene expression system.               Without Phenol Red.       72   SFM4CHO™ -Utility   Serum-free and protein-free   Supports growth of multiple   CHO               medium. Contains Pluronic   CHO cell clones and               F68. Available with or without   production of a variety of               L-Glutamine. Without Phenol   recombinant proteins for                   research, genomics and                   proteomics, in vitro diagnosis,                   drug target screening and                   validation, and manufacturing                   of preclinical lots.       73   HyQ PF 293   Serum-free expression   Expression medium for   Spodoptera (Sf9, Sf21)               medium.   baculovirus production and   Trichoplusia (High Five TM , Tn)                   recombinant protein   Drosophila                   purification. Promotes growth   Heliothis                   of cells from Spodoptera and   Other insect cells                   Trichoplusia, also Drosophila,                   Heliothis and other types of                   insect cells.            ICN BIOMEDICALS       www.icnbiomed.com                                    74   Serum-free Insect Virus   Complete ready to we serum-   Formulated for production of           Production Medium   free medium.   insect viruses.               With L-glutamine       75   Serum-free Medium,   Complete ready-to-use serum-   Formulated for mammalian   Various           Mammalian   free medium.   cell cultures.               With L-Glutamine.                                                                                                                                                                                 77   ICN-CHO   Serum-free, low protein,   Specifically developed for   CHO               complete medium.   rapid growth to high densities                   of CHO cells.       78   ICN-Hybridoma   Serum-free, very low protein,   Developed for culture of   Hybridoma               complete medium.   hybridoma cells and                   monoclonal antibody                   production.                                                                                                                                                                                 80   ICN-MRC-5   Serum-free, very low protein,   Developed for culture of   MRC-5               medium.   MRC-5 cells.                                                                                                                                                                                 82   Cellvation™   Serum-free and DMSO-free   Cryopreservation.   Various               cryopreservation medium.       83   TCH™   Completely defined serum   Developed primarily for   Human: 6T-CEM 20 (T-cell               replacement. Add to basal   human cells and production of   leukaemia), A375 (malignant               medium to replace serum.   cell-secreted proteins. Can   melanoma), A549 (lung                   also be used to culture   carcinoma), Burkitt&#39;s Lymphoma.                   mammalian cells from other   EBV transformed B cells,                   species. Long-term culture of   Epithelial-human placenta,                   both anchorage dependent and   malignant, Epithelia-human                   suspension cultures   placenta benign, HeLa (cervix),                       Lymphocytes infected with HIV I                       or HIV II, H9 (T-cell lymphoma),                       H9/HTLV-IIIB (T-cell, HIV+),                       JAR (placenta), Lesch-Nyhan                       Syndrome Lymphoblast, Mo (T-                       cell leukaemia), MRC-5 (lung),                       MT4 (lymphocytes), PC3 (prostate                       adenocarcinoma), Primary sensory                       neuron precursors, Transformed                       human epithelial, U-937                       (histiocytic lymphoma), WI 38                       (lung), WIL-2-729-HF-2                       (B lymphoblast)                       Mouse &amp; Hamster,                       SP 2/0-Ag-14 (mouse myeloma)                       Mouse hybridomas                       CHO                       CTLL-2 (T-cell mouse tumour)       84   TCM™ and TCM Insulin Free   Completely defined, serum-   Multi-purpose serum   Human: A375, A549, DU 145,               free, serum replacement. Add   replacement for a wide range   HEP-2, Human lymphocytes               to basal medium to replace   of cell types from a variety of   infected with HIV I and HIV II,               serum,   species. Also primary cell   HeLa, H9, Lesch-Nyhan Syndrome                   cultures. Supports long-term   Lymphoblast, LNCaP, MCF-7,                   culture of both anchorage   MEL-14, MRC-5, MT4, NCI-H69,                   dependant and suspension   neuroblastomas, P3HR-1, Raji,                   cultures.   SK-MES-1, THPI, WIL-2-729-                       HF-2                       Rat &amp; Mouse: B168L6, B16F10,                       CTLL-2, PC-12, rat-2 fibroblast,                       3T3, transformed rat fibroblast,                       UMR 106 and 108, myelomas and                       derived hybridomas (P3X63                       Ag8.653, P3/NSI/1-Ag4-1,                       SP 2/O-Ag14).                       B95-8, Marmoset EDV                       transformed leukocytes                       MEL III (rhesus mammary gland)                       Vero                       Avian (chicken &amp; turkey)                       Chicken fibroblasts                       QT6 (Quail)                       Bovine adrenal cells                       Bovine kidney                       CHO Chinese hamster ovary                       MDCK (canine kidney)                       CRFK (feline kidney)                       PK15 (porcine kidney)                       Swine testicle                       Primary Cells: Amphibian renal                       cells,                       Bovine adrenal cells, aorta                       endothelial and kidney, Human                       ventricle, Mussel cells,                       freshwater glochidia, Neuronal                       cultures, Embryonic chick heart,                       Prostate carcinoma,                       Rabbit cornea, Stomach epithelial                       Type II epithelial-lung            IN VITRO SYSTEMS AND SERVICES       www.ivss.de/en                                                                                                                                                                                                              86   ProSYSTEM   Serum-free medium based on   Developed for use with   Hybridoma               RPMI (proSYSTEM ROI) or   MiniPERM, and hollow fiber               based on DMEM (proSYSTEM   systems for serum-free or               DOI).   reduced serum culture of                   hybridoma. Promotes high cell                   densities and antibody                   concentrations            IRVINE SCIENTIFIC       www,irvinesci.com       UK suppliers: Metachem Diagnostics Ltd at www.metachem.co.uk                                    87   IS-CHO™   Serum-free medium, with L-   Optimised to promote long-   CHO cell lines               Glutamine. Contains bovine-   term, high-density growth of               derived components. Without   CHO cells and expression of               hypoxanthine or thymidine   recombinant proteins. For use               Technical data sheets available   with dihydrofolate reductase               on request.   and other selection systems.                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     91   IS-293   Serum-free medium, with low   For long-term, high-density   293 human embryonic kidney cells               protein content (human   culture of 293 cells, used for   HeLa cells               transferrin and recombinant   production of adenovirus or   Myelomas               human insulin). Technical data   recombinant protein   Hybridomas               sheets available on request.   expression. Optimised to limit   Human breast cancer cells                   cell aggregation in suspension                   cultures. Can also be used for                   growth of other cells.                                                                                                                                                                                                                                                                                                                                                   94   IS-BAC   Serum-free and protein-free   Supports long-term growth of   Spodoptera frugiperda (SF9 &amp;               medium   insect cells for production of   SF12) Tricoplusia (BTI-TN-5BI-4                   insect virus and recombinant   and high Five TM  cells)                   DNA proteins.                                                                                                                                                                                 96   HB-GRO   Serum-free medium. Technical   Dual formulation with HB-   Human and murine hybridomas               data available of request.   PRO to support growth and                   production phases of                   hybridoma culture.                   HB-GRO is used for the initial                   growth of human and murine                   hybridomas from inoculation                   to maximal cell density       97   HB-PRO   Serum-free and very low   Dual formulation with HB-   Human and marine bybwidomas               protein medium. Technical date   GRO to support growth and               available of request.   production of hybridoma                   culture. HB-PRO supports                   hybridoma growth during                   antibody production phase.       98   HB-101   Serum-free medium kit       Murine and human hybridomas,               consisting of basal media and       myelomas, lymphoblastoid cells,               lyophilized supplement pack.       SP2/0 hybrids, P3 hybrids, some               Technical data available of       NS-1 cell lines, endothelial               request.       cells and murine cell hybrids.       99   HB-104   Serum-free kit consisting of       Human hybridomas, human               basal media and lyophilized       myelomas, lymphoblastoid cells,               supplement pack. Technical       human T-cells, human B-cells,               data available on request.       lymphocytes and LAK cells.            JRH Biosciences       www.jrhbio.com                                    100   EX-CELL™ 301   Low protein. serum-free   Supports growth and   CHO cells               medium, Contains L-   maintenance of genetically               Glutamine, HEPES, sodium   engineered CHO cells in large-               bicarbonate, Pluronic F-68,   scale suspension culture, and               glucose, hypoxanthine,   when cells are attached to               thymidine, Phenol red.   microcarriers.               Animal-derived protein and               hydrolysate.       101   EX-CELL™ 302   Very low protein, serum-free   Supports CHO cells in   CHO cells               medium. Contains HEPES,   suspension culture for               sodium bicarbonate, Pluronic   expression of antibodies or               F-68, glucose. Human   protein products. Appropriate               recombinant protein and plant   for use with DHFR or GS               derived hydrolysate.   selection systems.               Without L-Glutamine,               hypoxanthine, and thymidine.       102   EX-CELL™ 325 PF CHO   Protein-free, serum-free   For growth of CHO cells and   CHO cells               medium. Contains HEPES,   expression of recombinant               sodium bicarbonate, Pluronic   products. Appropriate for use               F-68, glucose. Plant derived   with DHFR or GS selection               hydrolysate. Without L-   systems.               Glutamine, hypoxanthine, and               thymidine.       103   EX-CELL™ 400   Protein-free and serum-free   Designed for growth of cell   Spodoptera frugiperda, including               medium. Hydrolysate source   lines from Spodoptera   Sf19 and Sf23 cells               yeast. Without hypoxanthine   frugiperda. Can be used to               and thymidine.   propagate insect cells for                   expressing recombinant                   products. Can be used in both                   suspension and adherent                   culture systems.       104   EX-CELL™ 405   Protein-free and serum-free   Optimised for the nutrient and   Trichoplusia ni (High Five™ cells)               medium.   environmental needs of               Hydrolysate source yeast   Trichoplusia ni (High Five™               Without hypoxanthine and   cells). Can be used for               thymidine.   expressing recombinant                   products using the BEV                   system. Can be used in both                   suspension and adherent                   culture systems.       105   EX-CELL™ 420   Protein-free and serum-free   Optimised for growth of Sf9   Spodoptera frugiperda Sf9 and               medium. Contains L-   and Sf21 cells. Can be used in   Sf21 c Schneider S2 (Drosophila)               Glutamine, Pluronic-F-68, and   both suspension and adherent   cells               Glucose. Hydrolysate source   culture systems.               yeast.               Without hypoxanthine and               thymidine.       106   EX-CELL™ 520   Serum-free medium containing   Developed for production of   HEK293 cell               bovine serum albumin,   proteins and adenoviral               recombinant growth factors,   vectors using HEK293 cell               Pluronic F-68, and glucose.   line in suspension culture.               Without L-Glutamine.       107   EX-CELL™ VPRO   Serum-free medium, free of   Developed for long-term   PER.C6™ and related cell lines               animal protein.   growth of human embryo               With Pluronic F-68 and   retinoblast cells (PER.C6™               glucose, hypoxanthine and   and related cell lines) for               thymidine.   production of adenovirus.                   Cells can be grown as                   suspension cultures either in                   shaker flasks or roller bottles.       108   EX-CELL™ 293   Serum-free medium, free of   For long-term growth of HEX   HEX 293 cells               animal protein.   293 and related cells for               With Pluronic F-68 and   adenovirus production in               glucose. hypoxanthine and   suspension culture.               thymidine.       109   EX-CELL™ MDCK   Serum-free medium, free of   For long-term growth or   MDCK cells               animal protein.   MDCK and related cells in               With Pluronic F-68 and   attachment culture.               glucose, hypoxanthine and               thymidine.       110   EX-CELL™ 610-HSF   Low protein, serum-free,   Supports a wide range of cells   Lymphoid and epithelial cells and               chemically defined medium.   including lymphoid and   B cell hybridomas of murine, rat                   epithelial cells and B cell   and human origin                   hybridomas of murine, rat and                   human origin. Designed for                   production of antibodies.       111   EX-CELL™ 620-HSF   Low-protein, serum-free   Supports long-term growth of   Hybridoma and lymphoid cells               medium.   hybridoma and lymphoid cells,                   and is suited for the expression                   and isolation of monoclonal                   antibodies another protein                   products. Suitable for most                   cholesterol-dependent                   hybridoma cultures.            MD BIOSCIENCES       www.mdbiosciences.com                                    112   DCCM-1 &amp; DCCM-2   serum-free media, containing   Culture of myeloma and   Myeloma and hybridoma cells               BSA.   hybridoma cells, monoclonal   Human lymphocyte cells                   antibody production; culture of                   human lymphocyte cells                   (including stimulated and                   transformed cells); and virus                   production.       113   BIOGRO-1   Serum-free supplement, for   Culture of myeloma and   Myeloma and hybridonu cells               addition to basal medium.   hybridoma cells, monoclonal   Human lymphocyte cells               Contains albumin.   antibody production; culture of                   human lymphocyte cells                   (including stimulated and                   transformed cells); and virus                   production.       114   BIOGRO-2   Serum-free supplement, for   Culture of myeloma and   Myeloma and hybridonu cells               addition to basal medium. Very   hybridoma cells, monoclonal   Human lymphocyte cells               low protein and albumin-free.   antibody production; culture of                   human lymphocyte cells                   (including stimulated and                   transformed cells); and virus                   production.       115   BIOCHO-1 SFM Base   Serum-free basal medium.   Formulation for adherent CHO   CHO cells               Contains amino acids, salts,   cells.   CHO-KI and transfected cells               vitamins, lipids, and trace               elements. Requires addition of               BIOGRO-CHO SFM.       116   BIOCHO-2 SFM Base   Serum-free basal medium.   Formulation for CHO cells in   CHO cells               Contains amino acids, salts,   suspension   CHO-KI and transfected cells               vitamins, lipids, and trace               elements. Requires addition of               BIOGRO-CHO SFM.       117   BIOGRO-CHO SFM   Serum-free supplement for   Supplement for addition to   CHO cells               addition to BIOCHO-1 and   BIOCHO-1 &amp; 2 basal media.   CHO-KI and transfected cells               BIOCHO-2 SFM. Contains               proteins.            MABIO       www.mabio.net                                                                                                                                                                                                                                                                                                                                                                                120   00201/RM-B00   As above (00210/CTM-H00)   Expansion of human, murine,   For human, primate and murine               but with Bovine Serum   and simian hematopoietic stem   cells: Bone marrow               Albumin in place of human   and progenitor cells.   Umbilical cord blood               albumin.       Normal or mobilized peripheral                       blood Mononuclear cells                       Selected cells [CD34+ and sub                       populations]                                                                                                                                                                                 122   00202/RM-B00α   As above (00212/CTM-HOOα)   Expansion of human, murine   Human, primate and murine cells               but with Bovine Serum   and simian hematopoietic stem   Bone marrow               Albumin in place of human   and progenitor cells.   Umbilical cord blood               albumin.       Normal or mobilized peripheral                       blood Mononuclear cells                       Selected cells [CD34+ and sub                       populations]                                                                                                                                                                                 124   00203/RM-B01   As above (00211/CTM-H01)   Expansion of human   Human cells:               but with Bovine Serum   hematopoietic primitive   Bone marrow               Albumin in place of human   progenitor cells.   Umbilical cord blood               albumin.       Normal or mobilized peripheral                       blood Mononuclear cells                       Selected cells [CD34+ and sub                       populations]                                                                                                                                                                                 126   00205/RM-B03   As above (00216/CTM-H03)   Expansion of human   Human cells:               but with Bovine Serum   megakaryocytic   Bone marrow               Albumin in place of human   progenitor cells.   Umbilical cord blood               albumin.       Normal or mobilized peripheral                       blood Mononuclear cells                       Selected cells [CD34+ and sub                       populations]                                                                                                                                                                                 128   00206/RM-B04   As above (00213/CTM-HO4)   Expansion of human   Human cells:               but with Bovine Serum   hematopoietic myeloid   Bone marrow               Albumin in place of human   progenitor cells.   Umbilical cord blood               albumin.       Normal or mobilized peripheral                       blood Mononuclear cells                       Selected cells [CD34+ and sub                       populations]                                                                                                                                                                                                                                                                                                                                                   131   00207/RM-B05   Defined liquid medium. serum-   Expansion of human   Human cells:               free. Contains bovine serum   hematopoietic erythoid   Bone marrow               albumin, synthetic iron carrier,   progenitor cells   Umbilical cord blood               rb-insulin, nucleosides, L-       Normal or mobilized peripheral               glutamine, synthetic lipids, α-       blood Mononuclear cells               monothioglycerol, synthetic       Selected cells [CD34+ and sub               Iscove base medium. Cytokines       populations]               rhSCF, rhEPO, RhIL-3, rhIL-6,               rhIL-9, rhIL-11.       132   00204/RM-B02   Defined liquid medium. serum-   Expansion of total human   Human cells:               free. Contains bovine serum   hematopoietic   Bone marrow               albumin, synthetic iron carrier,   progenitor cells   Umbilical cord blood               rb-insulin, nucleosides, L-       Normal or mobilized peripheral               glutamine, synthetic lipids, α-       blood Mononuclear cells               monothioglycerol, synthetic       Selected cells [CD34+ and sub               Iscove base medium. Cytokines       populations]               rhSCF, rhTPO, rhFLT3-ligand,               rhG-CSF, rhEPO, rhIL-               1α, rhIL-3, rhIL-6, rhIL-11.            MEDIATECH INC       www.cellgro.com                                    133   cellgro Complete™ Serum Free   Complete serum-free medium.   Designed for hybridoma and   Hybridoma           Media   Based on 50/50 mix of   suspension cultures, but also               DMEM/F12, with smaller   supports some anchorage               percentage of RPMI 1640 and   dependent cell lines.               McCoy&#39;s 5A. Contains trace               elements, carbohydrates.               vitamins, non-animal protein,               small amoumt of BSA (1 gm/L).               No insulin, transferrin,               cholesterol, growth or               attachment factors.       134   cellgro FREE™ Serum-   Serum and protein-free   Designed for growth of   Mammalian cells, CHO.           free/Protein-free Media   medium, without hormones or   mammalian cell lines. Both               growth factors.   adherent and suspension                   culture. CHO K-1 require                   minimum adaptation into                   cellgro-FREE       135   Insectgro™   Serum-free and protein-free   For growth of drosophila S2   Insect S2 cells               medium.   cells            MEDICULT       www.medi-cult.dk                                    136   RenCyte™ System   Completely synthetic, protein-   Consists of four products   Wide range of cells               free media system based on   RenCyte 1-4 that enable               Medi-Cult&#39;s patented synthetic   researchers to design their own               serum replacement technology.   cell specific culture.       137   RenCyte™ BHK   Chemically defined, serum-free   Optimised for culture of baby   BHK               and protein-free medium.   hamster kidney cells (BHK)               Based on modified   and expression of recombinant               DMEM/Harris F12.   proteins.       138   RenCyte™ Hybridoma   Serum-free and protein-free   Optimised fro culture of   Hybridoma               medium.   hybridoma and expression of                   monoclonal antibodies       139   RenCyte™ CHO   Chemically defined, protein-   For cultivation of various   CHO cell lines               free and serum-free media.   CHO cell lines, both               Based on modified   suspension and adherent               DMEM/Harris F12. Does not   strains.               contain proteins of animal               origin.       140   RenCyte™ Fibroblast   Chemically defined, protein-   For cultivation of fibroblast   Fibroblast cells               free and serum-free media.   cells for the expression of               Based on modified   recombinant and life viral               DMEM/Harris F12. No   particles.               proteins of animal origin.       141   RenCyte™ CNS   Serum-free and protein-free   Experimentation with   Mammalian brain cells, including               medium.   mammalian brain cells.   cerebella granule cells, cortical                       neurones from rat brain, and PC12                       cells       142   RenCyte™ Freez   Chemically defined, protein-   Developed to facilitate   Range of cells               free and serum-free media.   freezing of cells in a serum-               Based on modified   free environment.               DMEM/Harris F12.            PAN BIOTECH       www.pan-biotech.de/en                                    143   Panserin™ -401   Defined serum-free medium.   All purpose medium for   All purpose medium.               Based on Iscove&#39;s medium,   cultivation of a variety of               contains transferrin, bovine   cells.               serum albumin, cholseterine,               lipids and trace elements.       144   Panserin™ -501   Defined serum-free low protein   Monoclonal antibody   Hybridoma               medium. Based on Iscove&#39;s   production.               medium, but without bovine               serum albumin.       145   Panserin™ -601   Defined serum-free, protein-   Useful as a conservation and   Lymphoid cells               reduced medium. Only protein   production medium for               is transferrin.   lymphoid cells.       146   Panserin™ -701   Serum-free medium, based on   Developed for the cultivation   Lymphocytes               Iscove&#39;s with BSA, transferrin,   of lymphocytes targeted for               lipids, selected trace elements   the chromosome preparations.               and phytohemaglutine.       147   Panserin™ -801   Serum-free medium, based on   Designed for culture of   Kertatinocytes               MCDB-153. enriched with   keratinocytes, and to prevent               supplements (epidermal growth   explosive growth of               factor, insulin, hydrocortisone,   fibroblasts.               ethanolamine, phosphate               ethanolamine, BPE)       148   Panserin™ -901   Serum-free adaptation medium.   Specially designed for                   demanding cells.            PGC SCIENTIFICS       www.pgcscientifics.com                                    149   COS Cell Serum-free System   Two media formulations - one   System consists of two media,   COS cells               serum-free growth medium and   a serum-free growth medium               one protein-free expression   and a protein-free medium for               medium. Growth medium   expression of recombinant               requires addition of serum   proteins by COS cells.               derived proteins (transferrin,               albumin, insulin and               lipoprotein, and other growth               factors)            PAN BIOTECH       www.pan-biotech.de/en                                    150   CYTO-SFI   Serum-free supplement to   For cultivation of hybridoma   Hybridoma               replace or reduce serum   cells               requirement. Contains insulin,               BSA, and complex mixture of               trace elements.       151   CYTO-SF2   Serum-free and protein-free   Especially for protein-free   Hybridoma               supplement to support growth   cultivation of hybridoma.               of hybridoma cells. Contains a               mixture of carbohydrates.               amino acids, vitamins, peptides               and trace elements. May be               used with any basal medium.       152   CYTO-SF3   Serum-free medium   To support the attachment   NIH-3T3                   dependent growth to NIH-3T3                   cells.       153   CYTO-SF4   Serum-free medium contains   Supports growth of human   Human leukaemia cell lines               insulin, albumin, and   leukaemia cells.   ATCC K562               transferrin. Prepared using               Iscove&#39;s modified DMEM as a               base formulation.       154   CYTO-SF5   Serum-free medium   Designed to support   HeLa                   attachment depended growth                   of HeLa cells. For long-term                   passage and cryopreservation.       155   CYTO-SF7   Serum-free medium   Designed for growing human   Caco-2                   colon adenocarcinoma cell line                   Caco-2       156   CYTO-SF10   Serum-free medium. Contains   For cultivation of African   COS               all human proteins.   green monkey cell line COS.                   Cells may be routinely sub-                   cultured and cryopreserved in                   the medium            PROMOCELL       www.promocell.com                                    157   MAM-PF   Chemically defined serum-free   Optimised for the growth of   CHO and BHK cells               medium, and proteins-free   CHO cells. BHK cells and               medium. Without L-glutamate.   expression of recombinant                   proteins in suspension culture.       158   CHO Express Media   Protein-free &amp; serum-free   For cultivation of CHO cells   CHO cells           SFC-60 &amp; SFC-70   media (formulation is   producing recombinant               confidential)   proteins.       159   CHO Express Media   Serum-free media (formulation   For cultivation of CHO cells.   CHO cells           SFC-20, SFC-30, SFC-40 &amp;   is confidential)   SFC-20 &amp; SFC-30 for           SFC-5O       anchorage dependent cells.                   and SFC-40 &amp; SFC-50                   suspension cultures.       160   Hybridoma Growth Medium 7   Fully defined medium, protein-   Cultivation of hybridoma   Various hybridoma               free, with or without phenol red       161   Hybridoma Growth Medium 6   Serum-free medium with L-   Cultivation of hybridoma   Various hybridoma               glutamate       162   SF-3 BaculoExpress ICM   Serum-free and protein free   Ready to use for cultivation of   Insect cells:               medium   insect cells   SF9, SF21, High Five™ and                       D.Mel-2       163   SF-1 BaculoExpress ICM   Serum-free media. Requires   For culture of insect cell lines   Sf9 and Sf21               supplementation with lipids   for production of recombinant               and Pluronic F68.   proteins in the baculo-virus                   expression system       164   IP301 BaculoExpress ICM   Chemically defined media,   For culture of insect cell lines   Sf9 and Sf21               serum-free media. Requires   for production of recombinant               supplementation with lipids   proteins in the baculo-virus               and Pluronic P68.   expression system       165   TB-1 Clonal Lymphocyte   Defined serum-free medium,       Lymphocytes           Medium   supplemented with albumin,               transferrin, lecithin,               ethanolamine and some fatty               acids. Contains selenium               pyruvate, potassium nitrate,               additional amino acids,               vitamins and HEPES buffer.       166   VPM-1 Virus Production   Serum-free and protein free   Growth of VERO cells.   VERO cells           Medium   medium.            QUALITY BIOLOGICAL, Inc.       www.qualitybiological.com       QBS media is also sold by Sigma-Aldrich Co.                                    167   QBSF-51   Serum-free, low protein   Designed to support growth of   COS, DA-1, HL-60, Jurkat, K-562,               medium consisting of Iscove&#39;s   transformed cells in   MLA, 144, Molt 4, Namalwa,               (IMDM) base, with HEPES,   suspension. Has also been   Neuro 2A, NIH 3T3, Raji, Wehi.               sodium bicarbonate, BSA,   used for colony selection and               human transferrin, rh insulin,   focus formation of NIH 3T3               L-Glutamine.   cells.       168   QBSF-52   Serum-free, low protein   Designed to support growth of   Hybridoma               medium of Iscove&#39;s (IMDM)   hybridomas and production of   ACT IV               base, with HEPES, sodium   monoclonal antibodies.               bicarbonate, lithium chloride.               BSA, human transferrin, rh               insulin. L-Glutamine.       169   QBSF-55   Serum-free, low protein   Designed to support growth of   Hybridoma               medium consisting of Iscove&#39;s   hybridomas and production of   Sp2/0-Ag14               (IMDM) base, with HEPES,   monoclonal antibodies.   NS-1               sodium bicarbonate, lithium               chloride, bovine serum               albumin, human transferrin,               human recombinant insulin,               cholesterol, L-Glutamine.       170   QBSF-56   Serum-free, low protein   Designed to support human   Human blood lymphocytes               medium consisting of Iscove&#39;s   peripheral blood blastogenesis   Sp2/0-Ag14               (IMDM) base, with HEPES,   and cell cultures. Has been   Sp2 SS1               sodium bicarbonate, bovine   used to support proliferation of   Sp2 SA3               serum albumin, human   primary cell cultures. Has been   Sp2 SA5               transferrin, human recombinant   successfully used to grow a   Sp2 MAI               insulin, cholesterol. L-   number of immunoglobulin   U-937               Glutamine.   secreting clones and other cell                   lines.       171   QBSF-58   Serum-free, low protein   Designed for expansion of   Human and murine bone marrow               medium consisting of Iscove&#39;s   human and murine bone   cells               (IMDM) base, with HEPES,   marrow cells.               sodium bicarbonate, bovine               serum albumin, human               transferrin, hr insulin,               cholesterol, L-Glutamine.       172   QBSF-59 &amp;   Serum-free media   Designed to support human   CD34+ cells derived front normal           QBSF-60       hematopoietic stem/progenitor   bone marrow, cord blood, and                   cells.   peripheral blood stern cells.            ROCHE       www.roche-applied.science.com                                    173   Nutridoma-CS   Defined medium supplement   Optimised for freshly fused   Hybridomas derived from SP 2/0,               that can be used to completely   hybridomas during selection   P3X63Ag8.653 and NS-1 myeloma               replace serum in cell culture   and cloning procedures in   cell lines.               medium (e.g. RPMI 1640).   serum-free cell culture.               Composed of albumin, insulin,               transferrin, cytokines,               cholesterol source and other               defined organic and inorganic               compounds.       174   Nutridoma-HU   Biochemically defined serum-   Supports the growth of most   Human myeloma and hybridoma               free supplement that can be   lymphoblastoid, myeloma and   cell lines and primary cultures of               used to completely replace   hybridoma cell lines, as well   human lymphoid cells.               serum in cell culture medium   as primary lymphoid cell               (e.g. DMEM/RPMI 1640).   cultures.               Composed of albumin, insulin,               transferrin, and other defined               organic can inorganic               compounds. Most proteins are               of human origin.       175   Nutridoma-NS   Biochemically defined, serum-   Supports the growth of most   NS-1 and P3X63-Ag8.653               free supplement that can be   lymphoblastoid. myeloma and   myeloma cell lines and their fusion               used to completely replace   hybridoma cell lines, as well   derived hybridomas. CHO cells               serum in cell culture medium   as primary lymphoid cell               (e.g. high glucose   cultures. Occasionally               DMEM/Ham&#39;s F12).   successful with non-lymphoid               Composed of albumin, insulin,   cell lines.               transferrin, and other defined   This NS formulation meets               organic can inorganic   nutritional requirements for               compounds. Plus a cholesterol   cell lines having a deficiency               source.   in the biosynthetic pathway for                   cholesterol       176   Nutridoma-SP   Biochemically defined, serum-   Supports murine myelomas   Murine Sp/2/0 myeloma cell lines               free supplement that can be   and hybridomas that have   and their fusion derived               used to completely replace   intact cholesterol biosynthesis   hybridomas. Neural explants               serum in cell culture medium   pathway. Also for culture of               (e.g. DMEM/RPMI 1640).   various other cell types,               Composed of albumin, insulin,   including neural explants.               transferrin, and other defined               compounds.            STEMCELL TECHNOLOGIES       www.stemcell.com                                    177   StemSpan™ SFEM   Serum-free expansion medium.   Developed for the culture and   Human hematopoietic progenitor               Components include bovine   expansion of human   cells               serum albumin, human   hematopoietic progenitor cells.               recombinant insulin, human   Optimised and tested using               transferrin (iron-saturated), 2-   CD34+ enriched cell               Mercaptoehtanol, L-Glutamine,   populations from normal               Iscove&#39;s MDM.   donors.       178   StemSpan™ H3000   Serum-free defined medium   Developed for culture of   Human hematopoietic cells               containing only pre-tested   human hematopoietic cells               human-derived or recombinant               human proteins. Requires               supplementing with               recombinant cytokines.       179   StemSpan™ BIT 9500   Serum substitute for use in   For use where media of   Human and murine hematopoietic               place of fetal bovine serum.   defined composition is   progenitor cells               Contains pre-tested batches of   required. For use in               bovine serum albumin, human   methylcellulose-based colony               recombinant insulin and human   assay of for expansion               transferrin (BIT). Plus   medium for human or murine               Iscove&#39;s MDM.   hematopoietic progenitors.            SIGMA-ALDRICH Co.       www.sigmaaldrich.com                                    180   CHO Serum-free Medium   Serum-free medium containing   Optimized for use in   CHO cells               inorganic salts, HEPES,   recombinant protein               sodium bicarbonate, essential   expression and production in               and non-essential amino acids,   Chinese hamster Ovary (CHO)               vitamin, bovine serum   cell systems.               albumin, human transferrin,               fetal bovine fetuin (USA               source) trace elements, phenol               red, Pluronic F-68, and other               organic compounds.               Without L-glutamine,               antibiotics, and antimycotics.               Also no hypoanthine or               thymidine.       181   Hybridoma Medium, Serum-   Serum-free medium containing   Supports high viable cell   Hybridoma           free   inorganic salts, essential and   densities and high antibody               non-essential amino acids,   productivity over extended               vitamins, sodium bicarbonate,   culture periods of 60 days or               HEPES, trace elements, fatty   greater. Suitable for cloning               acids and other organics.   and fusion applications.               Contains low concentrations of               bovine serum albumin and               human transferrin. Does not               contain phenol red, L-               glutamine, antibiotics, and               antimycotics.       182   Serum-free and Protein-free   Based on a modification of   Designed to support growth of   Hybridoma           Hybridoma Medium   Ham&#39;s Nutrient Mixture F-12   hybridomas, but may not               containing additional   support myelomas which               components and MOPS   require cholesterol               buffering system. Full formula               available.       183   MDBK-GM-SF   Serum-free, low protein   This medium together with the   MDBK cells           Madin-Darby Bovine Kidney   medium containing inorganic   protein-free version below, are           Growth Medium Serum-free   salts, HEPES and sodium   designed to support optimal               carbonate buffers, essential and   growth of MDBK cells for               non-essential amino acids,   production of viruses useful               vitamins, recombinant human   for vaccines, in two phases:               insulin and growth factors,   the growth phase and the high-               fetuin, transferrin, other   density maintenance and virus               organic compounds and trace   production phase.               elements. Without L-glutamine.   MDBK-GM-SF is designed to                   support growth of MDBK                   cells before transfer into the                   protein-free medium below.                   Recommended for use with                   roller bottle and microcarrier                   bead based bioreactor system.       184   MDBK-MM-PF   Serum-free, protein-free   Designed far maintenance of   MDBK cells           Madin-Darby Bovine Kidney   medium containing inorganic   MDBX cells in high densities           Maintenance Medium Protein-   salts, HEPES and sodium   over extended periods of time,           free   carbonate buffers, essential and   and for virus production.               non-essential amino acids,   Recommended for use with               vitamins, recombinant human   roller bottle and micro carrier               insulin and growth factors,   bead based bioreactor system.               fetuin, transferrin, other               organic compounds and trace               elements. Without L-glutamine.       185   MDCK-SF   Serum-free medium containing   Designed to support the   MDCX cells           Madin-Darby Canine Kidney   inorganic, salts, essential and   growth of Madin-Darby canine           Medium, Serum-free   non-essential amino acids,   kidney cells that support the               vitamins, recombinant human   production of viruses useful as               insulin and growth factors,   vaccines.               other organic compounds and               trace elements. Contains bovine               serum albumin. Without L-               glutamine       186   MDCK-PF   Serum-free medium containing   Designed to support the   MDCK cells           Madin-Darby Canine Kidney   inorganic salts, essential and   growth of Madin-Darby canine           Medium, Protein-free   non-essential amino acids,   kidney cells that support the               vitamins, recombinant human   production of viruses useful as               insulin and growth factors,   vaccines.               other organic compounds and               trace elements. Without L-               glutamine.                                                                                                                                                                                 188   Serum-free Insect Medium-1   Serum-free and protein-free   Developed for production of   Insect cells           Protein-free   medium with L-glutamine and   recombinant proteins.               sodium bicarbonate. Based on               IPL-41 medium originally               developed by Weiss et al at the               USDA Insect Pathology               Laboratory. Contains inorganic               salts, amino acids, vitamins,               other organic compounds and               trace elements.       189   Serum-free Insect Medium-2   Serum-free and low-protein   Developed for production of   Insect cells           Low-protein   medium with L-glutamine and   recombinant proteins.               sodium bicarbonate. Based on               IPL-41 medium originally               developed by Weiss et al at the               USDA Insect Pathology               Laboratory. Contains inorganic               salts, amino acids, vitamins,               other organic compounds and               trace elements.                                                                                                                                                                                 191   Stemline™ Serum-free   Semi-solid serum-free medium   For assay of human   Human hematopoietic cells           Methylcellulose Medium   consists of Iscove&#39;s Modified   clonogenic hematopoietic           Without Growth Factors,   Dulbecco&#39;s Medium,   progenitor cells isolated from           Human   methylcellulose, bovine serum   bone marrow or other               albumin, L-Glutamine, 2-   hematopoietic cells. For               Mercaptoethanol, rh insulin,   enumeration and evaluation of               human transferrin, iron-   stem cell derived progeny               saturated. Does not contain   characterised as colony               erythropoietin or other   forming units.               cytokines.       192   Stemline™ Serum-free   Semi-solid serum-free medium   For assay of murine   Mouse hematopoietic cells           Methylcellulose Medium   consists of Iscove&#39;s Modified   clonogenic hematopoietic           Without Growth Factors,   Dulbecco&#39;s Medium,   progenitor cells isolated from           Mouse   pharmaceutically grade   murine bone marrow or other               methylcellulose, bovine serum   hematopoietic cells. For               albumin, L-Glutamine, 2-   enumeration and evaluation of               Mercaptoethanol, rh insulin,   stem cell derived progeny               human transferrin, iron-   characterised as colony               saturated. Does not contain   forming units.               erythropoietin or other               cytokines.       193   Cell Freezing Medium-DMSO,   Serum-free medium for   Designed to protect and           Serum-Free   cryopreservation, containing   preserve cells during frozen               8.7% DMSO in MEM   storage.               supplemented with               methylcellulose.       194   Cell Freezing Medium Serum-   Serum-free medium for   Designed to protect and           Free   cryopreservation, without   preserve cells during frozen               DMSO   storage.                                                                                                                                                                                                                                                                                                                                                     BSA = bovine serum albumin            EGF = epidermal growth factor            rhEGF = human recombinant epidermal growth factor            FGF = fibroblast growth factor            hrbFGF = human recombinant basic fibroblast growth factor            GM-CSF = granulocyte-macrophage colony-stimulating factor (can be human recombinant)             
 
         [0213]    
       
         
               
               
               
               
               
             
           
               
                 TABLE II 
               
               
                   
               
               
                   
               
               
                   
                 Species of 
                   
                   
                   
               
               
                 Antigenic Determinant 
                 Hybridoma 
                 Isotype 
                 Name 
                 Catalog No. 
               
               
                   
               
             
             
               
                 Abelson murine leukemia virus, 16-kDa antigen 
                 rat/mouse 
                 IgG2a 
                 CDR1 
                 HB-213 
               
               
                 abl oncogene peptide, synthetic 
                 mouse 
                 IgG1 and IgG2b 
                 310-29F7 
                 CRL-2656 
               
               
                 abl oncogene peptide, synthetic 
                 mouse 
                 IgG1 and IgG2b 
                 311-3D4 
                 CRL-2657 
               
               
                 abl oncogene peptide, synthetic 
                 mouse 
                 IgG1 
                 312-13E8 
                 CRL-2658 
               
               
                 Acetylcholine receptor (AChR) alpha subunit 
                 rat 
                 IgG2a 
                 mAb64 
                 HB-8987 †   
               
               
                 Acetylcholine receptor (AChR) alpha subunit 
                 rat/mouse 
                 IgG1 
                 mAb 35 
                 HB-8857 †   
               
               
                 Acetylcholine receptor (AChR) alpha subunit 
                 rat/mouse 
                 IgG1 
                 mAb35 
                 TIB-175 
               
               
                 Acetylcholine receptor, neuronal, chicken 
                 rat/mouse 
                 IgG2a 
                 mAb 270 
                 HB-189 
               
               
                 Acetylcholine receptor, neuronal, rat 
                 rat/mouse 
                 IgG2a 
                 mAb 270 
                 HB-189 
               
               
                 Acetylcholinesterase, human 
                 mouse 
                 IgG1 
                 AE-1 
                 HB-72 
               
               
                 Acetylcholinesterase, human 
                 mouse 
                 IgG1 
                 AE-2 
                 HB-73 
               
               
                 Acid phosphatase, prostatic (PAP), human 
                 mouse 
                 IgG1 
                 RLTM01 
                 HB-8526 †   
               
               
                 Acid phosphatase, prostatic (PAP), human 
                 mouse 
                 IgG1 
                 RLTM02 
                 HB-8523 †   
               
               
                 Actin 
                 mouse 
                 IgG1 
                 ACT I 
                 HB-80 
               
               
                 Actin 
                 mouse 
                 IgG1 
                 ACT IV 
                 HB-81 
               
               
                 Actinin, alpha 
                 mouse 
                 IgM 
                 G-3-5 
                 CRL-2252 
               
               
                 Addressin, mucosal vascular, mouse 
                 rat/mouse 
                 IgG2a 
                 MECA-89 
                 HB-292 
               
               
                 Addressin, peripheral node, mouse 
                 rat/mouse 
                 IgM 
                 MECA-79 
                 HB-9479 †   
               
               
                 Adenocarcinoma, colon, human 
                 mouse 
                 IgG1 
                 CLT 85 
                 HB-8240 †   
               
               
                 Adenocarcinoma, colon, human 
                 mouse 
                 IgG3 
                 HT 29/36 
                 HB-8248 †   
               
               
                 Adenovirus group-specific antigen 
                 mouse 
                 IgG2a 
                 2Hx-2 
                 HB-8117 †   
               
               
                   Agrobacterium tumefaciens  biovar 3 
                 mouse 
                 IgG1 
                 F21-1D3G7C8 
                 HB-9463 †   
               
               
                 Aldosterone 
                 mouse 
                 IgG1 
                 A2E11 
                 CRL-1846 
               
               
                 Alpha fetoprotein (AFP), human 
                 mouse 
                 IgG1; kappa 
                 OM 3-1.1 
                 HB-134 
               
               
                 Alpha-1,3-dextran 
                 mouse 
                 IgA; lambda 
                 J558 
                 TIB-6 
               
               
                 Alveolar surfactant protein (ASP) 
                 mouse 
                 IgG 
                 DS-1 
                 HB-8906 †   
               
               
                 Alveolar surfactant protein (ASP) 
                 mouse 
                 IgG1 
                 DS-3 
                 HB-8651 †   
               
               
                 Alveolar surfactant protein (ASP) 
                 mouse 
                 IgG1 
                 DS-5 
                 HB-8653 †   
               
               
                 Alveolar surfactant protein (ASP) 
                 mouse 
                 IgG1 
                 DS-6 
                 HB-8652 †   
               
               
                 Amylase, salivary, human 
                 mouse 
                 IgG2a 
                 110-5 
                 HB-8984 †   
               
               
                 Angiotensin-converting enzyme (ACE) 
                 mouse 
                 IgM 
                 α-ACE 3.1.1 
                 HB-8191 †   
               
               
                 Annexin I, human 
                 mouse 
                 IgG1 
                 EH17a 
                 CRL-2209 
               
               
                 Annexin I, human 
                 mouse 
                 IgG1 
                 EH7a 
                 CRL-2194 
               
               
                 Annexin II, human 
                 mouse 
                 IgG1 
                 EH7a 
                 CRL-2194 
               
               
                 Antigen-dependent killer (K) cells, human 
                 mouse 
                 IgM; kappa 
                 HNK-1 
                 TIB-200 
               
               
                 AP-2 adaptor protein of clathrin coated vesicles 
                 mouse 
                 IgG1 
                 AP.6 
                 CRL-2227 
               
               
                 Apolipoprotein A-I (Apo-A-I), human 
                 mouse 
                 IgG1 
                 A5.4 
                 CRL-2275 
               
               
                 Apolipoprotein E (ApoE), human 
                 mouse 
                 IgG1 
                 WU E-14 
                 CRL-2255 
               
               
                 Apolipoprotein E (ApoE), human 
                 mouse 
                 IgG1 
                 WU E-4 
                 CRL-2247 
               
               
                 Asialo GM1 
                 mouse 
                 IgM 
                 SH-34 
                 CRL-2405 
               
               
                 Asialo GM2 
                 mouse 
                 IgM 
                 2D4 
                 TIB-185 
               
               
                 Astrocyte protein, human 
                 mouse 
                 IgM 
                 J1-31 
                 CRL-2253 
               
               
                 Astrocyte, rat 
                 mouse 
                 IgG2a 
                 RAN-2 
                 TIB-119 
               
               
                 Astrocytoma cell line, human 
                 mouse 
                 IgG2a 
                 G253 
                 HB-9706 †   
               
               
                 Astrocytoma cell line, human 
                 mouse 
                 IgG1 
                 K117 
                 HB-8553 †   
               
               
                 Astrocytoma cell line, human 
                 mouse 
                 IgG1 
                 S5 
                 HB-9255 †   
               
               
                 Astrovirus group antigen 
                 mouse 
                 IgG1 
                 7F2-6D4-8E7 
                 HB-11945 †   
               
               
                 ATPase, rat (Na, K dependent) 
                 mouse 
                 IgG1 
                 9-A5 
                 CRL-1844 
               
               
                 ATPase, rat (Na, K dependent) 
                 mouse 
                 IgG1 
                 9-B1 
                 CRL-1845 
               
               
                 Autocrine growth factor, 15 kDa, human 
                 mouse 
                 IgM 
                 CBL-1 
                 HB-8214 †   
               
               
                 B cell antigen (p50), mouse 
                 rat/mouse 
                 IgM 
                 RA3-2C2/1 
                 TIB-145 
               
               
                 B cell derived malignancies, human 
                 mouse 
                 IgG2a 
                 Lym-1 
                 HB-8612 †   
               
               
                 B cell growth factor 1, mouse 
                 rat/mouse 
                 IgG1 
                 11B11 
                 HB-188 
               
               
                 B cell precursors, mouse 
                 rat/mouse 
                 IgG2b 
                 14.8 
                 TIB-164 
               
               
                 B cell stimulatory factor 1, mouse 
                 rat/mouse 
                 IgG1 
                 11B11 
                 HB-188 
               
               
                 B cells, bovine 
                 mouse 
                 IgG2a 
                 CC56 
                 HB-273 
               
               
                 B cells, human 
                 mouse 
                 IgG2a 
                 Lym-1 
                 HB-8612 †   
               
               
                 B lymphocytes, mouse 
                 rat/mouse 
                 IgM 
                 J11d.2 
                 TIB-183 
               
               
                 B220, mouse 
                 rat/mouse 
                 IgM 
                 RA3-3A1/6.1 
                 HB-146 
               
               
                 B7.1, mouse 
                 hamster/mouse 
                 IgG 
                 16-10A1 
                 HB-301 
               
               
                 B7.1, mouse 
                 rat/mouse 
                 IgG2a 
                 IG10 
                 CRL-2223 
               
               
                 B7.2, mouse 
                 rat/mouse 
                 IgG2b 
                 2D10 
                 CRL-2226 
               
               
                 B7.2, mouse 
                 rat/mouse 
                 IgG2a 
                 GL1 
                 HB-253 
               
               
                 Basal cells (skin), human 
                 mouse 
                 IgG1 
                 VM-2 
                 HB-8530 †   
               
               
                 BCGF-1, mouse 
                 rat/mouse 
                 IgG1 
                 11B11 
                 HB-188 
               
               
                 Bicoid (bcd) protein,  Drosophila melanogaster   
                 mouse 
                 IgG1 
                 bcd mab23 
                 CRL-2107 
               
               
                 Blood group A antigen 
                 human 
                 IgM 
                 HAA1 
                 HB-8534 †   
               
               
                 Bluetongue virus VP7 
                 mouse 
                 IgG2b 
                 7D3A.2 
                 CRL-1886 
               
               
                 Bluetongue virus VP7 
                 mouse 
                 IgG2a 
                 8A3B.6 
                 CRL-1875 
               
               
                 Bluetongue virus VP7 
                 mouse 
                 IgG2b 
                 8B1B.1 
                 CRL-1877 
               
               
                 Bovine herpesvirus 1 (BHV-1) 
                 bovine/mouse 
                 IgG1 
                 αBL5C2.870005 
                 HB-9907 †   
               
               
                 Bovine herpesvirus 1 (BHV-1) 
                 bovine/mouse 
                 IgG1 
                 αBL5C2.870009 
                 HB-9908 †   
               
               
                 Bovine herpesvirus 1 (BHV-1) 
                 bovine/mouse 
                 IgG1 
                 αBL5C2.870016 
                 HB-9909 †   
               
               
                 Bovine herpesvirus 1 (BHV-1) 
                 mouse 
                 IgG1 
                 1B8-F11 
                 CRL-1852 
               
               
                 Bovine herpeavirus 1 (BHV-1) 
                 mouse 
                 IgG2b 
                 2H6-C2 
                 CRL-1853 
               
               
                 Bp35 (B cell antigen), human 
                 mouse 
                 IgG2a 
                 IF5 
                 HB-9645 †   
               
               
                 Bp50 (B cell antigen), human 
                 mouse 
                 IgG1 
                 G28-5 
                 HB-9110 †   
               
               
                 Breast cancer cells, human 
                 mouse 
                 IgG1 
                 317G5.C1D3 
                 HB-8691 †   
               
               
                 Breast cancer cells, human 
                 mouse 
                 IgG2a 
                 454C11 
                 HB-8484 †   
               
               
                 Breast cancer cells, human 
                 mouse 
                 IgG1 
                 520C9 
                 HB-8696 †   
               
               
                 Breast cancer cells, human 
                 mouse 
                   
                 650E2-2B12 
                 HB-10812 †   
               
               
                 BSF-1, mouse 
                 rat/mouse 
                 IgG1 
                 11B11 
                 HB-188 
               
               
                 Bubonic plague bacillus 
                 mouse 
                 IgA 
                 F1-3G8-1 
                 HB-192 
               
               
                 C3d receptor (CR2), human 
                 mouse 
                 IgG2a; kappa 
                 THB-5 
                 HB-135 
               
               
                 Canine adenovirus type 1 (CAV-1) 
                 mouse 
                 IgG1 
                 2E10-H2 
                 CRL-1812 
               
               
                 Canine adenovirus type 2 (CAV-2) 
                 mouse 
                 IgG2a 
                 4H1-A7 
                 CRL-1813 
               
               
                 Canine distemper virus (CDV) 
                 mouse 
                 IgG1 
                 CDC 1C42H11 
                 HB-216 
               
               
                 Carcinoembryonic antigen (CEA) 
                 mouse 
                 IgM 
                 1116NS-3d 
                 CRL-8019 
               
               
                 Carcinoembryonic antigen (CEA) 
                 mouse 
                 IgG1; kappa 
                 T84.66A3.1A.1F2 
                 HB-8747 †   
               
               
                 Carcinoma cells, human 
                 mouse 
                 IgG3 
                 KC-4G3 
                 HB-8709 †   
               
               
                 Carcinoma cells, human 
                 mouse 
                 IgM 
                 KC-4M1 
                 HB-8710 †   
               
               
                 Carcinoma associated antigen, heat stable, human 
                 mouse 
                 IgG2a 
                 AS 33 
                 HB-8779 †   
               
               
                 CD1, bovine 
                 mouse 
                 IgG2a 
                 CC20 
                 HB-267 
               
               
                 CD1, human 
                 mouse 
                 IgG1 
                 OKT 6 
                 CRL-8020 
               
               
                 CD1, pig 
                 mouse 
                 IgG2a; kappa 
                 76-7-4 
                 HB-140 
               
               
                 CD1.1, mouse 
                 rat/mouse 
                 IgG2b; kappa 
                 15C6 
                 HB-326 
               
               
                 CD1.1, mouse 
                 rat/mouse 
                 IgG2b; kappa 
                 15F7 
                 HB-322 
               
               
                 CD1.1, mouse 
                 rat/mouse 
                 IgG2b; kappa 
                 19F8 
                 HB-321 
               
               
                 CD1.1, mouse 
                 rat/mouse 
                 IgG1; kappa 
                 20H2 
                 HB-323 
               
               
                 CD1.1, mouse 
                 rat/mouse 
                 IgG2b 
                 4C4 
                 HB-327 
               
               
                 CD1w2, bovine 
                 mouse 
                 IgG2a 
                 CC20 
                 HB-267 
               
               
                 CD2, bovine 
                 mouse 
                 IgG1 
                 CC42 
                 HB-272 
               
               
                 CD2, bovine 
                 mouse 
                 IgG2a 
                 IL-A42 
                 CRL-1870 
               
               
                 CD2, human 
                 mouse 
                 IgG2a 
                 35.1 
                 HB-222 
               
               
                 CD2, human 
                 mouse 
                 IgG1 
                 OKT 11 
                 CRL-8027 
               
               
                 CD2, human 
                 mouse 
                 IgG1 
                 TS2/18.1.1 
                 HB-195 
               
               
                 CD2, sheep 
                 mouse 
                 IgG2a 
                 36F-18C 
                 HB-285 
               
               
                 CD3 epsilon chain, human 
                 mouse 
                 IgG2b 
                 BC3 
                 HB-10166 †   
               
               
                 CD33, human 
                 mouse 
                 IgG2a 
                 M195 
                 HB-10306 †   
               
               
                 CD34, human 
                 mouse 
                 IgG1; kappa 
                 AC133.1 
                 HB-12346 †   
               
               
                 CD35, human 
                 mouse 
                 IgG1; kappa 
                 Mab 543 
                 HB-8592 †   
               
               
                 CD38, human 
                 mouse 
                 IgG1 
                 OKT 10 
                 CRL-8022 
               
               
                 CD38, human 
                 mouse 
                 IgG1 
                 THB-7 
                 HB-136 
               
               
                 CD40 ligand (CD154, CD40L), human 
                 mouse 
                 IgG1 
                 hCD40L-M90 
                 HB-12055 †   
               
               
                 CD40 ligand (CD154, CD40L), human 
                 mouse 
                 IgG1 
                 hCD40L-M91 
                 HB-12056 †   
               
               
                 CD40 ligand, human 
                 mouse 
                 IgG2a 
                 5c8 
                 HB-10916 †   
               
               
                 CD40 ligand, mouse 
                 hamster/mouse 
                 IgG 
                 MR1 
                 CRL-2580 
               
               
                 CD40, human 
                 mouse 
                 IgG2b 
                 3A8 
                 HB-12024 †   
               
               
                 CD40, human 
                 mouse 
                 IgG1 
                 G28-5 
                 HB-9110 †   
               
               
                 CD44, human 
                 mouse 
                 IgG2a 
                 Hermes-3 
                 HB-9480 †   
               
               
                 CD44, mouse 
                 rat/mouse 
                 IgG1 
                 KM114 
                 TIB-242 
               
               
                 CD44, mouse 
                 rat/mouse 
                 IgG1 
                 KM201 
                 TIB-240 
               
               
                 CD44, mouse 
                 rat/mouse 
                 IgG2a 
                 KM703 
                 CRL-1896 
               
               
                 CD44, mouse 
                 rat/mouse 
                 IgG2a 
                 KM81 
                 TIB-241 
               
               
                 CD44, mouse 
                 rat/mouse 
                 IgG2a 
                 LYK-12 
                 HB-316 
               
               
                 CD44, mouse 
                 rat/mouse 
                 IgG2a 
                 LYK-16 
                 HB-319 
               
               
                 CD44, mouse 
                 rat/mouse 
                 IgG1 
                 LYK-5 
                 HB-310 
               
               
                 CD44, mouse, isoforms expressing variable 
                 rat/mouse 
                 IgG1 
                 LYK-1 
                 HB-306 
               
               
                 exon V10 
               
               
                 CD44, mouse, isoforms expressing variable 
                 rat/mouse 
                 IgG1 
                 LYK-7 
                 HB-311 
               
               
                 exon V10 
               
               
                 CD44, mouse, isoforms expressing variable 
                 rat/mouse 
                 IgG2a 
                 LYK-8 
                 HB-312 
               
               
                 exon V10 
               
               
                 CD44, mouse, isoforms expressing variable 
                 rat/mouse 
                 IgG2a 
                 LYK-9 
                 HB-313 
               
               
                 exon V10 
               
               
                 CD44, v4 variant, human 
                 mouse 
                 IgG2a 
                 FW11-10-3 
                 HB-257 
               
               
                 CD44, v6 variant, human 
                 mouse 
                 IgG2a 
                 FW11-9-2 
                 HB-256 
               
               
                 CD44, v9 variant, human 
                 mouse 
                 IgG1 
                 FW11-24-17-36 
                 HB-258 
               
               
                 CD45, human 
                 mouse 
                 IgG2a 
                 4B2 
                 HB-196 
               
               
                 CD45, human 
                 mouse 
                 IgG2a 
                 9.4 
                 HB-10508 †   
               
               
                 CD45, human 
                 mouse 
                 IgG2a; kappa 
                 GAP 8.3 
                 HB-12 
               
               
                 CD45, mouse 
                 rat/mouse 
                 IgG2b 
                 M1/89.18.7.HK 
                 TIB-124 
               
               
                 CD45, mouse 
                 rat/mouse 
                 IgG2a 
                 M1/9.3.4.HL.2 
                 TIB-122 
               
               
                 CD45, mouse 
                 rat/mouse 
                 IgG2a 
                 MB23G2 
                 HB-220 
               
               
                 CD45, mouse 
                 rat/mouse 
                 IgG2a 
                 MB4B4 
                 HB-223 
               
               
                 CD45, pig 
                 mouse 
                 IgM; kappa 
                 74-9-3 
                 HB-156 
               
               
                 CD45R, mouse 
                 rat/mouse 
                 IgM 
                 RA3-3A1/6.1 
                 TIB-146 
               
               
                 CD45RA, mouse 
                 rat/mouse 
                 IgG2b 
                 14.8 
                 TIB-164 
               
               
                 CD45RC, mouse 
                 rat/mouse 
                 IgM 
                 I/24.D6 
                 HB-251 
               
               
                 CD47, human 
                 mouse 
                 IgG1 
                 B6H12.2 
                 HB-9771 
               
               
                 CD49a, human 
                 mouse 
                 IgG1 
                 TS2/7.1.1 
                 HB-245 
               
               
                 CD49d, sheep 
                 mouse 
                 IgG2b 
                 FW3-218-1 
                 HB-261 
               
               
                 CD54, mouse 
                 rat/mouse 
                 IgG2a 
                 BE29G1 
                 HB-233 
               
               
                 CD57, human 
                 mouse 
                 IgM; kappa 
                 HNK-1 
                 TIB-200 
               
               
                 CD58, human 
                 mouse 
                 IgG1 
                 TS2/9.1.4.3 
                 HB-205 
               
               
                 CD62E, human 
                 mouse 
                 IgG2a; kappa 
                 CL2 
                 CRL-2514 
               
               
                 CD62E, human 
                 mouse 
                 IgG1; kappa 
                 CL3 
                 CRL-2515 
               
               
                 CD62E, human 
                 mouse 
                 IgG1; kappa 
                 CL37 
                 CRL-2516 
               
               
                 CD62E, human 
                 mouse 
                 IgG2a 
                 H18/7 
                 HB-11684 †   
               
               
                 CD62L, human 
                 mouse 
                 IgG2a 
                 1H3 
                 HB-284 
               
               
                 CD62L, human 
                 mouse 
                 IgG1 
                 DREG200 
                 HB-302 
               
               
                 CD62L, human 
                 mouse 
                 IgG1 
                 DREG56 
                 HB-300 
               
               
                 CD62L, mouse 
                 rat/mouse 
                 IgG2a 
                 MEL-14 
                 HB-132 
               
               
                 CD62L, sheep and bovine 
                 mouse 
                 IgG1 
                 DU1-29 
                 HB-263 
               
               
                 CD62P, human 
                 mouse 
                 IgG1 
                 WAPS 12.2 
                 HB-299 
               
               
                 CD80, mouse 
                 hamster/mouse 
                 IgG 
                 16-10A1 
                 HB-301 
               
               
                 CD117, human 
                 mouse 
                 IgG2a 
                 BA7.3C.9 
                 HB-10716 †   
               
               
                 CDw128, human 
                 mouse 
                 IgG2a 
                 10H2.12.1 
                 HB-11494 †   
               
               
                 CDw128, human 
                 mouse 
                 IgG2a 
                 4D1.5.7 
                 HB-11495 †   
               
               
                 CD151, human 
                 mouse 
                 IgG1 
                 41-2 
                 CRL-2695 
               
               
                 CD151, human 
                 mouse 
                 IgG1 
                 50-6 
                 CRL-2696 
               
               
                 CD152, mouse 
                 hamster/mouse 
                 IgG 
                 UC10-4F10-11 
                 HB-304 
               
               
                 CD154, human 
                 mouse 
                 IgG2a 
                 5c8 
                 HB-10916 †   
               
               
                 CD154, mouse 
                 hamster/mouse 
                 IgG 
                 MR1 
                 CRL-2580 
               
               
                 Cell surface antigen on bovine periodontal 
                 mouse 
                 IgM 
                 PDL-1 
                 CRL-1882 
               
               
                 ligament cells 
               
               
                 Cell surface antigen on human myeloma cells 
                 mouse 
                 IgG1 
                 D 14 
                 HB-8439 †   
               
               
                 (M-8 antigen system) 
               
               
                 Centromere protein B (CENP-B), human 
                 mouse 
                 IgG1 
                 2D-7 
                 HB-9667 †   
               
               
                 Cervical carcinoma, human 
                 human 
                 IgG1; kappa 
                 CLN H11.4 
                 HB-8307 †   
               
               
                 Cervical carcinoma, human 
                 human 
                 IgM 
                 CLNH5.5 
                 HB-8206 †   
               
               
                 Channel catfish immunoglobulin 
                 mouse 
                 IgG1; kappa 
                 E-8 
                 HB-10179 †   
               
               
                 Chlamydia genus-specific antigen 
                 mouse 
                 IgG2b; kappa 
                 89MS30 
                 HB-11300 †   
               
               
                 Cholesterol 
                 mouse 
                 IgM 
                 2C5-6 
                 HB-8995 †   
               
               
                 Choriocarcinoma tumor cell antigen, human 
                 mouse 
                 IgG2a 
                 K66 
                 HB-8767 †   
               
               
                 Choriocarcinoma tumor cell antigen, human 
                 mouse 
                 IgG1 
                 SV63 
                 HB-8766 †   
               
               
                 Choriocarcinomas, human 
                 mouse 
                 IgG1 (Igh-4a allotype) 
                 162-46.2 
                 HB-187 
               
               
                 Chronic lymphocytic leukemia (CLL) 
                 mouse 
                 IgG1 
                 Lym-2 
                 HB-8613 †   
               
               
                 Class II antigen, beta chain, mouse 
                 hamster/mouse 
                 IgG 
                 KL277 
                 CRL-2030 
               
               
                 Class II antigen, beta chain, mouse 
                 mouse 
                 IgG1 
                 KL295 
                 CRL-1996 
               
               
                 Class II antigen, beta chain, mouse 
                 mouse 
                 IgG2b 
                 KL304 
                 CRL-2027 
               
               
                 Clathrin, bovine (brain) 
                 mouse 
                 IgM 
                 CVC.4 
                 TIB-137 
               
               
                 Clathrin, heavy chain, human 
                 mouse 
                 IgG1 
                 TD.1 
                 CRL-2232 
               
               
                 Clathrin, heavy chain, human 
                 mouse 
                 IgG1 
                 X22 
                 CRL-2228 
               
               
                 Clathrin, light chain, bovine (brain) 
                 mouse 
                 IgG1 
                 CVC.1 
                 TIB-135 
               
               
                 Clathrin, light chain, bovine (brain) 
                 mouse 
                 IgG2a 
                 CVC.7 
                 TIB-138 
               
               
                 Clathrin, light chain, human 
                 mouse 
                 IgG2b 
                 CON.1 
                 CRL-2229 
               
               
                 Colchicine 
                 mouse 
                 IgG2a 
                 C44 
                 CRL-1943 
               
               
                 Collagen, bone type 1 
                 mouse 
                 IgG1 
                 1H11 
                 HB-10611 †   
               
               
                 Colon carcinoma-associated antigens (CCAA), 
                 mouse 
                 IgG1; kappa 
                 PCA 31.1 
                 HB-12314 †   
               
               
                 human 
               
               
                 Colon carcinoma-associated antigens (CCAA), 
                 mouse 
                 IgG2a; kappa 
                 PCA 33.28 
                 HB-12315 †   
               
               
                 human 
               
               
                 Colon cells, 29-kDa glycoprotein, human 
                 mouse 
                 IgG2a 
                 HT 29/26 
                 HB-8247 †   
               
               
                 Colon tumor-associated antigen (CTAA) 16.88 
                 human 
                 IgG3; kappa 
                 CO 88BV59-1 
                 CRL-10624 †   
               
               
                 Colon, adenocarcinoma, human 
                 mouse 
                 IgG1 
                 CLT 85 
                 HB-8240 †   
               
               
                 Colon, adenocarcinoma, human 
                 mouse 
                 IgG3 
                 HT 29/36 
                 HB-8248 †   
               
               
                 Colonic mucin glycoprotein, human 
                 mouse 
                 IgG2a 
                 UC7 
                 HB-9753 †   
               
               
                 Colonic protein, human 
                 mouse 
                 IgM 
                 7E12H12 
                 HB-9397 †   
               
               
                 Colony stimulating factor, subclass I (CSF-I), human 
                 mouse 
                 IgG1 
                 F18 AF1 
                 HB-8208 †   
               
               
                 Colony stimulating factor, subclass I (CSF-I), human 
                 mouse 
                 IgG1 
                 F1A3-23 
                 HB-8207 †   
               
               
                 Colorectal carcinoma monosialoganglioside 
                 mouse 
                 IgG1 
                 1116-NS-19-9 
                 HB-8059 †   
               
               
                 Colorectal carcinoma-associated tumor antigen 
                 mouse 
                 IgG2b 
                 XMMCO-791 
                 HB-9173 †   
               
               
                 Common leukocyte antigen, human 
                 mouse 
                 IgG2a 
                 4B2 
                 HB-196 
               
               
                 Common leukocyte antigen, mouse 
                 rat/mouse 
                 IgG2b 
                 M1/89.18.7.HK 
                 TIB-124 
               
               
                 Common leukocyte antigen, mouse 
                 rat/mouse 
                 IgG2a 
                 M1/9.3.4.HL2 
                 TIB-122 
               
               
                 Common leukocyte antigen, mouse 
                 rat/mouse 
                 IgG2a 
                 MB23G2 
                 HB-220 
               
               
                 Common leukocyte antigen, mouse 
                 rat/mouse 
                 IgG2a 
                 MB4B4 
                 HB-223 
               
               
                 Complement C1q, human 
                 mouse 
                 IgG 
                 12A5B7 
                 HB-8328 †   
               
               
                 Complement C1q, human 
                 mouse 
                 IgG 
                 4A4B11 
                 HB-8327 †   
               
               
                 Complement C3b receptor, human 
                 mouse 
                 IgG1; kappa 
                 Mab 543 
                 HB-8592 †   
               
               
                 Concanavalin A (Con A) 
                 mouse 
                 IgG1 
                 71A7 
                 TIB-147 
               
               
                 Cortical thymic epithelium, mouse 
                 rat/mouse 
                 IgG2a 
                 CDR1 
                 HB-213 
               
               
                 Cortical thymocytes, mouse 
                 rat/mouse 
                 IgM 
                 J11d.2 
                 TIB-183 
               
               
                 Coxsackievirus B4 
                 mouse 
                 IgG2a; kappa 
                 204-4 
                 HB-185 
               
               
                 Coxsackievirus B4 
                 mouse 
                 IgG2a; kappa 
                 339-1 
                 HB-186 
               
               
                 Coxsackievirus B4 
                 mouse 
                 IgG2a; kappa 
                 356-1 
                 HB-181 
               
               
                 Coxsackievirus B4 
                 mouse 
                 IgG2a; kappa 
                 38-1 
                 HB-182 
               
               
                 Coxsackievirus-adenovirus receptor (CAR), human 
                 mouse 
                 IgG1 
                 RmcB 
                 CRL-2379 
               
               
                 CR1, human 
                 mouse 
                 IgG1; kappa 
                 Mab 543 
                 HB-8592 †   
               
               
                 CD3, human 
                 mouse 
                 IgM 
                 38.1 
                 HB-231 
               
               
                 CD3, human 
                 mouse 
                 IgG2a 
                 OKT 3 
                 CRL-8001 
               
               
                 CD3, mouse 
                 hamster/mouse 
                 IgG 
                 145-2C11 
                 CRL-1975 
               
               
                 CD4, bovine 
                 mouse 
                 IgG1 
                 CC30 
                 HB-270 
               
               
                 CD4, bovine 
                 mouse 
                 IgG2a 
                 CC8 
                 HB-280 
               
               
                 CD4, bovine 
                 mouse 
                 IgG2a 
                 IL-A11 
                 CRL-1879 
               
               
                 CD4, human 
                 mouse 
                 IgG2b 
                 OKT 4 
                 CRL-8002 
               
               
                 CD4, mouse 
                 rat/mouse 
                 IgG2b 
                 GK1.5 
                 TIB-207 
               
               
                 CD4, sheep 
                 mouse 
                 IgG1 
                 17D 
                 HB-262 
               
               
                 CD4a, pig 
                 mouse 
                 IgG2b; kappa 
                 74-12-4 
                 HB-147 
               
               
                 CD4-binding domain of the gp120 protein of HIV-1 
                 human/mouse 
                 IgG1 
                 448-D 
                 HB-10895 †   
               
               
                 CD4-binding domain of the gp120 protein of HIV-1 
                 human/mouse 
                 IgG1 
                 558-D 
                 HB-10894 †   
               
               
                 CD4-binding domain of the gp120 protein of HIV-1 
                 human/mouse 
                 IgG1 
                 559/64-D 
                 HB-10893 †   
               
               
                 CD5, bovine 
                 mouse 
                 IgG1 
                 CC17 
                 HB-281 
               
               
                 CD5, bovine 
                 mouse 
                 IgG1 
                 CC29 
                 HB-269 
               
               
                 CD5, human 
                 mouse 
                 IgG1 
                 OKT 1 
                 CRL-8000 
               
               
                 CD6, bovine 
                 mouse 
                 IgG2b 
                 CC38 
                 HB-266 
               
               
                 CD6, human 
                 mouse 
                 IgG2a 
                 12.1 
                 HB-228 
               
               
                 CD6, human 
                 mouse 
                 IgM 
                 3Pt12B8 
                 HB-8136 †   
               
               
                 CD7, human 
                 mouse 
                 IgG1; kappa 
                 T3-3A1 
                 HB-2 
               
               
                 CD8, bovine 
                 mouse 
                 IgG1 
                 CC58 
                 HB-275 
               
               
                 CD8, bovine 
                 mouse 
                 IgG2a 
                 CC63 
                 HB-264 
               
               
                 CD8, bovine 
                 mouse 
                 IgG1 
                 IL-A51 
                 CRL-1871 
               
               
                 CD8, human 
                 mouse 
                 IgG2a 
                 51.1 
                 HB-230 
               
               
                 CD8, human 
                 mouse 
                 IgG2a 
                 OKT 8 
                 CRL-8014 
               
               
                 CD8, human 
                 mouse 
                 IgG1 
                 S6F1 
                 HB-9579 †   
               
               
                 CD8 alpha 2.2, mouse 
                 mouse 
                 IgM 
                 83-12-5 
                 CRL-1971 
               
               
                 CD9, mouse 
                 rat/mouse 
                 IgG2a 
                 KMC8.8 
                 CRL-2212 
               
               
                 CD11a, human 
                 mouse 
                 IgG1 
                 TS2/4.1.1 
                 HB-244 
               
               
                 CD11a, mouse 
                 rat/mouse 
                 IgG2b 
                 FD441.8 
                 TIB-213 
               
               
                 CD11a, mouse 
                 rat/mouse 
                 IgG2a; kappa 
                 M17/4.4.11.9 (new clone 
                 TIB-217 
               
               
                   
                   
                   
                 of M17/4.2) 
               
               
                 CD11a, mouse 
                 rat/mouse 
                 IgG2b; kappa 
                 M17/5.2 
                 TIB-237 
               
               
                 CD11b, human 
                 mouse 
                 IgM; kappa 
                 17aba 
                 HB-248 
               
               
                 CD11b, human 
                 mouse 
                 IgG2a; kappa 
                 44aacb 
                 HB-249 
               
               
                 CD11b, human 
                 mouse 
                 IgG1 
                 LM2/1.6.11 
                 HB-204 
               
               
                 CD11b, human 
                 mouse 
                 IgG2b 
                 OKM 1 
                 CRL-8026 
               
               
                 CD11b, mouse 
                 rat/mouse 
                 IgG2b 
                 5C6 Clone 1 
                 CRL-1969 
               
               
                 CD11c, mouse 
                 hamster/mouse 
                 IgG 
                 N418 
                 HB-224 
               
               
                 CD14, human 
                 mouse 
                 IgG2b; kappa 
                 26ic 
                 HB-246 
               
               
                 CD14, human 
                 mouse 
                 IgG2b 
                 3C10 
                 TIB-228 
               
               
                 CD14, human 
                 mouse 
                 IgG1; kappa 
                 60bca 
                 HB-247 
               
               
                 CD18, human 
                 mouse 
                 IgG2a; lambda 
                 IB4 
                 HB-10164 †   
               
               
                 CD18, human 
                 mouse 
                 IgG1 
                 TS1/18.1.2.11 
                 HB-203 
               
               
                 CD18, mouse 
                 hamster/mouse 
                 IgG 
                 2E6 
                 HB-226 
               
               
                 CD18, mouse 
                 rat/mouse 
                 IgG2a; kappa 
                 M18/2.a.12.7 (new 
                 TIB-218 
               
               
                   
                   
                   
                 clone of M18/2.a.8) 
               
               
                 CD19, mouse 
                 rat/mouse 
                 IgG2a; kappa 
                 1D3 
                 HB-305 
               
               
                 CD20, human 
                 mouse 
                 IgG2a 
                 1F5 
                 HB-9645 †   
               
               
                 CD20, human 
                 mouse 
                 IgG1 
                 C273 
                 HB-9303 †   
               
               
                 CD21, bovine 
                 mouse 
                 IgG2b 
                 CC51 
                 HB-271 
               
               
                 CD21, human 
                 mouse 
                 IgG2a; kappa 
                 THB-5 
                 HB-135 
               
               
                 CD25, human 
                 mouse 
                 IgG2a 
                 7G7B6 
                 HB-8784 †   
               
               
                 CD25, mouse 
                 rat/mouse 
                 IgM; kappa 
                 7D4 
                 CRL-1698 
               
               
                 CD25, mouse 
                 rat/mouse 
                 IgG1 
                 PC 61 5.3 
                 TIB-222 
               
               
                 CD28 receptor, mouse 
                 hamster/mouse 
                 IgG 
                 PV1 
                 HB-12352 †   
               
               
                 CD29, human 
                 mouse 
                 IgG1 
                 TS2/16.2.1 
                 HB-243 
               
               
                 CD29, mouse 
                 rat/mouse 
                 IgG2a 
                 KMI6 
                 CRL-2179 
               
               
                 CD29, sheep 
                 mouse 
                 IgG1 
                 FW4-101-1-1 
                 HB-289 
               
               
                 CD32, human 
                 mouse 
                 IgG2b 
                 IV.3 
                 HB-217 
               
               
                 CD32, mouse 
                 rat/mouse 
                 IgG2b 
                 2.4G2 
                 HB-197 
               
               
                 C-reactive protein, human 
                 mouse 
                 IgG2a; kappa 
                 HD2-4 
                 HB-86 
               
               
                 Creatine kinase - MM and MB, human 
                 rat/mouse 
                 IgG2a; kappa 
                 CKMM 14.15 
                 HB-9419 †   
               
               
                 Creatine kinase - MM, human 
                 rat/mouse 
                 IgA; kappa 
                 CKMM 14.5 
                 HB-9420 †   
               
               
                 Creatine kinase - MM, human 
                 rat/mouse 
                 IgG1; kappa 
                 CKMM 14.52 
                 HB-9421 †   
               
               
                 CTLA-4, mouse 
                 hamster/mouse 
                 IgG 
                 UC10-4F10-11 
                 HB-304 
               
               
                 Cutaneous lymphocyte antigen (CLA), human 
                 rat/mouse 
                 IgM 
                 HECA-452 
                 HB-11485 †   
               
               
                 Cutaneous melanocytes (M-10 antigen system), 
                 mouse 
                 IgG1 
                 M 144 
                 HB-8440 †   
               
               
                 human 
               
               
                 Cutaneous melanocytes (M-24 antigen system), 
                 mouse 
                 IgG1 
                 M-24 (M138) 
                 HB-8449 †   
               
               
                 human 
               
               
                 Cutaneous melanocytes (M-25 antigen system), 
                 mouse 
                 IgG2b 
                 L368 
                 HB-8450 †   
               
               
                 human 
               
               
                 Cutaneous melanocytes (M-4 antigen system), 
                 mouse 
                 IgG1 
                 M 111 
                 HB-8438 †   
               
               
                 human 
               
               
                 Cystic fibrosis transmembrane conductance 
                 mouse 
                 IgG1 
                 mAb 13-1 
                 HB-10565 †   
               
               
                 regulator (CFTR) 
               
               
                 Cystic fibrosis transmembrane conductance 
                 mouse 
                 IgG2a; kappa 
                 mAB 24-1 
                 HB-11947 †   
               
               
                 regulator (CFTR) 
               
               
                 Cystic fibrosis transmembrane conductance 
                 mouse 
                 IgG1; kappa 
                 mAB 24-2 
                 HB-11946 †   
               
               
                 regulator (CFTR) 
               
               
                 Cytokeratin 18 (CK18) 
                 mouse 
                 IgG 
                 UCD/PR 10.11 
                 HB-8694 †   
               
               
                 Cytokeratin 8 (CK8) 
                 mouse 
                 IgG 
                 UCD/PR 10.11 
                 HB-8694 †   
               
               
                 Cytomegalovirus (HCMV) UL18 heavy chain, human 
                 mouse 
                 IgG1 
                 10C7 
                 CRL-2430 
               
               
                 Cytomegalovirus (HCMV), immediate —early 
                 mouse 
                 IgG1 
                 L-14 
                 HB-8554 †   
               
               
                 antigen, human 
               
               
                 Cytomegalovirus (MCMV) m144 heavy chain, 
                 mouse 
                 IgG1 
                 15C6 
                 CRL-2431 
               
               
                 mouse 
               
               
                 DEC-205, human 
                 mouse 
                 IgG2b 
                 MG38 
                 CRL-2640 
               
               
                 DEC-205, mouse 
                 rat/mouse 
                 IgG2a 
                 DEC-205 
                 HB-290 
               
               
                 Delta heavy chain, human 
                 mouse 
                 IgG3; kappa 
                 δTA4-1 
                 HB-70 
               
               
                 Dendritic cell antigen, human 
                 mouse 
                 IgG2b 
                 MG38 
                 CRL-2640 
               
               
                 Dendritic cell antigen, mouse 
                 rat/mouse 
                 IgG2a 
                 DEC-205 
                 HB-290 
               
               
                 Dendritic cells, mouse 
                 rat/mouse 
                 IgG2b 
                 33D1 
                 TIB-227 
               
               
                 Dengue virus complex 
                 mouse 
                 IgG2a 
                 D3-2H2-9-21 
                 HB-114 
               
               
                 Dengue virus type 1 
                 mouse 
                 IgG1 
                 15F3-1 
                 HB-47 
               
               
                 Dengue virus type 3 
                 mouse 
                 IgG1 
                 5D4-11 
                 HB-49 
               
               
                 Dengue virus type 4 
                 mouse 
                 IgG1 
                 1H10-6 
                 HB-48 
               
               
                 Dengue virus-2, type specific determinant 
                 mouse 
                 IgG1 
                 3H5-1 
                 HB-46 
               
               
                 Dinitrophenyl (DNP) 
                 hamster/mouse 
                 IgG 
                 UC8-1B9 
                 CRL-1968 
               
               
                 Dioxins 
                 mouse 
                 IgG2a; kappa 
                 DD-4 
                 HB-9743 †   
               
               
                 Diphtheria toxin 
                 human/mouse 
                 IgG 
                 16M3F10 
                 HB-8363 †   
               
               
                 Disialosyl Lea (tumor associated fucoganglioside) 
                 mouse 
                 IgG3 
                 FHCR-1-2516/FH7 
                 HB-8861 †   
               
               
                 DNA (single stranded) 
                 mouse 
                 IgG3 
                 MRSS-1 (D 2 D 4 ) 
                 HB-69 
               
               
                 DNA polymerase alpha, human 
                 mouse 
                 IgG1 
                 SJK-132-20 
                 CRL-1640 
               
               
                 DNA polymerase alpha, human 
                 mouse 
                 IgG1 
                 SJK-237-71 
                 CRL-1645 
               
               
                 DNA polymerase alpha, human 
                 mouse 
                 IgG1 
                 SJK-287-38 
                 CRL-1644 
               
               
                 DNA polymerase alpha, human 
                 mouse 
                 IgG1 
                 STK 1 
                 CRL-1652 
               
               
                 DNA polymerase epsilon (pol epsilon), human 
                 mouse 
                 IgG2a 
                 3C5.1 
                 CRL-2284 
               
               
                 DNA polymerase III holoenzyme,  Escherichia coli   
                 mouse 
                 IgM 
                 123-10 
                 CRL-1707 
               
               
                 DNA polymerase III holoenzyme,  Escherichia coli   
                 mouse 
                 IgG1 
                 123-28 
                 CRL-1713 
               
               
                 DNA polymerase III holoenzyme,  Escherichia coli   
                 mouse 
                 IgM 
                 68-1-2 
                 CRL-1712 
               
               
                 DNA, double stranded 
                 mouse 
                 IgM 
                 CH26-1352 
                 HB-8329 †   
               
               
                 DNP and TNP substituted proteins 
                 mouse 
                 IgA; lambda 2 
                 MOPC 315 
                 TIB-23 
               
               
                 EGF receptor 
                 mouse 
                 IgG1 
                 225 
                 HB-8508 †   
               
               
                 EGF receptor 
                 mouse 
                 IgG1 
                 455 
                 HB-8507 †   
               
               
                 EGF receptor 
                 mouse 
                 IgG2a 
                 528 
                 HB-8509 †   
               
               
                 EGF receptor 
                 mouse 
                 IgG 
                 579 
                 HB-8506 †   
               
               
                 EGF receptor, human 
                 mouse 
                 IgM 
                 Mab 96 
                 HB-9763 †   
               
               
                   Eimeria tenella  sporozoites 
                 mouse 
                 IgG1 
                 S1E4 
                 HB-8332 †   
               
               
                   Eimeria tenella  sporozoites 
                 mouse 
                 IgG2a 
                 S3D3 
                 HB-8331 †   
               
               
                   Eimeria tenella  sporozoites and merozoites 
                 mouse 
                 IgG1 
                 13.90.2 
                 HB-8337 †   
               
               
                   Eimeria tenella  sporozoites and merozoites 
                 mouse 
                 IgG2a 
                 2.03.7 
                 HB-8389 †   
               
               
                 ELAM-1, human 
                 mouse 
                 IgG2a; kappa 
                 CL2 
                 CRL-2514 
               
               
                 ELAM-1, human 
                 mouse 
                 IgG1; kappa 
                 CL3 
                 CRL-2515 
               
               
                 ELAM-1, human 
                 mouse 
                 IgG1; kappa 
                 CL37 
                 CRL-2516 
               
               
                 ELAM-1, human 
                 mouse 
                 IgG2a 
                 H18/7 
                 HB-11684 †   
               
               
                 EM10 
                 mouse 
                 IgM; kappa 
                 SM27-1045 
                 HB-11917 †   
               
               
                 Endothelial cells, IL-1 activated, human 
                 mouse 
                 IgG1 
                 7A9 
                 HB-10135 †   
               
               
                 Endothelial cells, peripheral lymph node, mouse 
                 rat/mouse 
                 IgM 
                 MECA-79 
                 HB-9479 †   
               
               
                 Endothelial leukocyte adhesion molecule 1 
                 mouse 
                 IgG2a; kappa 
                 CL2 
                 CRL-2514 
               
               
                 (ELAM-1), human 
               
               
                 Endothelial leukocyte adhesion molecule 1 
                 mouse 
                 IgG1; kappa 
                 CL3 
                 CRL-2515 
               
               
                 (ELAM-1), human 
               
               
                 Endothelial leukocyte adhesion molecule 1 
                 mouse 
                 IgG1; kappa 
                 CL37 
                 CRL-2516 
               
               
                 (ELAM-1), human 
               
               
                 Endothelial leukocyte adhesion molecule 1 
                 mouse 
                 IgG2a 
                 H18/7 
                 HB-11684 †   
               
               
                 (ELAM-1), human 
               
               
                 Endothelium, human 
                 mouse 
                 IgG1; kappa 
                 10B9 
                 HB-172 
               
               
                 Endothelium, human 
                 mouse 
                 IgG1; kappa 
                 14E5 
                 HB-174 
               
               
                 Ependymal cell, rat 
                 mouse 
                 IgG2a 
                 RAN-2 
                 TIB-119 
               
               
                 Epidermal growth factor (EGF) receptor 
                 mouse 
                 IgG1 
                 225 
                 HB-8508 †   
               
               
                 Epidermal growth factor (EGF) receptor 
                 mouse 
                 IgG1 
                 455 
                 HB-8507 †   
               
               
                 Epidermal growth factor (EGF) receptor 
                 mouse 
                 IgG2a 
                 528 
                 HB-8509 †   
               
               
                 Epidermal growth factor (EGF) receptor 
                 mouse 
                 IgG 
                 579 
                 HB-8506 †   
               
               
                 Epidermal growth factor (EGF) receptor, human 
                 mouse 
                 IgM 
                 Mab 96 
                 HB-9763 †   
               
               
                 Epidermis, basal layer, fetal and neonatal, human 
                 mouse 
                 IgG1 
                 DAL K20 
                 CRL-2288 
               
               
                 Epidermis, basal layer, fetal and neonatal, human 
                 mouse 
                 IgG1 
                 DAL K29 
                 CRL-2291 
               
               
                 Epithelial cells, gastrointestinal tract mucosa, 
                 mouse 
                 IgG1 
                 CLT 152 
                 HB-8244 †   
               
               
                 52-kDa protein, human 
               
               
                 Epithelium, human 
                 mouse 
                 IgM; kappa 
                 Ep-16 
                 HB-155 
               
               
                 Epstein-Barr virus (EBV) 
                 mouse 
                 IgG1 
                 72A1 
                 HB-168 
               
               
                 Epstein-Barr virus (EBV) receptor 
                 mouse 
                 IgG2a; kappa 
                 THB-5 
                 HB-135 
               
               
                 Equine infectious anemia virus (EIAV) core 
                 mouse 
                 IgG1 
                 EIAV 12E8.1 
                 HB-8917 †   
               
               
                 antigen (p26) 
               
               
                 erb B (v-erb B) oncogene peptide, synthetic 
                 mouse 
                 IgG1 
                 171-11B9 
                 CRL-2661 
               
               
                 erb B (v-erb B) oncogene peptide, synthetic 
                 mouse 
                 IgG1 
                 172-12A4 
                 CRL-2660 
               
               
                 erb B (v-erb B) oncogene peptide, synthetic 
                 mouse 
                 IgG1; kappa 
                 173-1C11 
                 CRL-2659 
               
               
                 erb B2 (c-erb B2) protein, human 
                 mouse 
                 IgG1; kappa 
                 20.3 
                 CRL-2655 
               
               
                 erb B-2 protein, human 
                 mouse 
                 IgG1 
                 Ab 21.1 
                 HB-11601 †   
               
               
                 erb B-2 protein, human 
                 mouse 
                 IgG1 
                 Ab 23.1 
                 HB-11602 †   
               
               
                 Erythrocytes, mouse 
                 rat/mouse 
                 IgM 
                 J11d.2 
                 TIB-183 
               
               
                 Erythropoietin 
                 mouse 
                 IgG1 
                 5F12 AD3 
                 HB-8209 †   
               
               
                 Erythropoietin, human 
                 rat/mouse 
                 IgG2a 
                 BF-11 
                 CRL-8164 †   
               
               
                   Escherichia coli  0157:H7 strain 932 
                 mouse 
                 IgG2a 
                 4E8C12 
                 HB-10452 †   
               
               
                 Farnesyltransferase, alpha subunit 
                 mouse 
                 IgG1 
                 IgG-IB7 
                 CRL-2418 
               
               
                 Fas antigen, human 
                 mouse 
                 IgG1 
                 huFasM3 
                 HB-11726 †   
               
               
                 Fas antigen, human 
                 mouse 
                 IgG1 
                 huFasM38 
                 HB-11465 †   
               
               
                 Fc alpha receptor, human 
                 mouse 
                 IgM 
                 My 43.51 
                 HB-12128 †   
               
               
                 Fc gamma receptor, high affinity, human 
                 mouse 
                 IgG1; kappa 
                 CT6-1D7 
                 CRL-2438 
               
               
                 Fc gamma receptor, mouse 
                 rat/mouse 
                 IgG2b 
                 2.4G2 
                 HB-197 
               
               
                 FcRI, human 
                 mouse 
                 IgG1; kappa 
                 CT6-1D7 
                 CRL-2438 
               
               
                 FcRI, human 
                 mouse 
                 IgM 
                 My 43.51 
                 HB-12128 †   
               
               
                 FcRII, mouse 
                 rat/mouse 
                 IgG2b 
                 2.4G2 
                 HB-197 
               
               
                 FcRn heavy chain heterodimers 
                 mouse 
                 IgG1 
                 1G3 
                 CRL-2434 
               
               
                 FcRn heavy chain heterodimers 
                 mouse 
                 IgG1 
                 2G3 
                 CRL-2435 
               
               
                 Feline leukemia virus (FeLV), p27 protein 
                 mouse 
                 IgG 
                 24IA 2 E 2 E 10 D 5   
                 HB-8049 †   
               
               
                 Fibrin, human 
                 mouse 
                   
                 F45J 
                 HB-9740 †   
               
               
                 Fibrin, human 
                 mouse 
                 IgG1; kappa 
                 MH1 
                 HB-9739 †   
               
               
                 Fibrinogen, human 
                 mouse 
                   
                 F45J 
                 HB-9740 †   
               
               
                 Fibronectin, human 
                 mouse 
                 IgG1 
                 HFN 36.3 
                 CRL-1605 
               
               
                 Fibronectin, human 
                 mouse 
                 IgG1 
                 HFN 7.1 
                 CRL-1606 
               
               
                 Fibronectin, human 
                 mouse 
                 IgG1 
                 P 3 NP/PFn 
                 HB-91 
               
               
                 Fibronectin, human, onco-fetal determinant 
                 mouse 
                 IgG1 
                 FHCR-1-2813/FDC-6 
                 HB-9018 †   
               
               
                 Fimbriae (2134P) of enterotoxigenic  E. coli   
                 mouse 
                 IgG1 
                 αM346C7C1 
                 HB-11124 †   
               
               
                 Flavivirus group antigen 
                 mouse 
                 IgG2a 
                 D1-4G2-4-15 
                 HB-112 
               
               
                 Flk-1/KDR 
                 rat/mouse 
                 IgG1; kappa 
                 DC101 
                 HB-11534 †   
               
               
                 Follicle stimulating hormone (FSH) receptor, human 
                 mouse 
                 IgG1 
                 FSHR-18 
                 CRL-2688 
               
               
                 Forssman antigen 
                 rat/mouse 
                 IgM 
                 M1/22.25.8.HL 
                 TIB-121 
               
               
                 Forssman antigen 
                 rat/mouse 
                 IgM 
                 M1/87.27.7.HLK 
                 TIB-123 
               
               
                 fos oncogene peptide, synthetic 
                 mouse 
                 IgG2b; kappa 
                 411-14E10 
                 CRL-2663 
               
               
                 fos oncogene peptide, synthetic 
                 mouse 
                 IgG1 and IgG2b 
                 413-15D12 
                 CRL-2653 
               
               
                 Gamma heavy chain, human 
                 mouse 
                 IgG1; kappa 
                 1410 KG7 
                 HB-43 
               
               
                 Gamma heavy chain, human 
                 mouse 
                 IgG2b; lambda 
                 C3-124 
                 HB-60 
               
               
                 6B Ganglioside (tumor-associated fucoganglioside) 
                 mouse 
                 IgM 
                 FHCR-1-2624/FH6/ 
                 HB-8873 †   
               
               
                   
                   
                   
                 FHOT-1-3019 
               
               
                 Ganglioside associated with endocrine cells, 
                 mouse 
                 IgM; kappa 
                 3G5 
                 CRL-1814 
               
               
                 human T lymphocytes, and neuronal cells 
               
               
                 Ganglioside GD2 
                 mouse 
                 IgM 
                 Mab 126 
                 HB-8568 †   
               
               
                 Gangliosides GD2 and GD3 
                 mouse 
                 IgG2a 
                 ME361S2a 
                 HB-9326 †   
               
               
                 Gangliosides GM3 and GM4, human 
                 human 
                 IgM; kappa 
                 L612 
                 CRL-10724 †   
               
               
                   Giardia muris  trophozoites 
                 mouse 
                 IgG3; kappa 
                 1A3.1 
                 CRL-1961 
               
               
                   Giardia muris  trophozoites 
                 mouse 
                 IgG2b; kappa 
                 2B5.3 
                 CRL-1960 
               
               
                   Giardia muris  trophozoites 
                 mouse 
                 IgG1; kappa 
                 3C7.2 
                 CRL-1959 
               
               
                 Glioblastoma, human 
                 mouse 
                 IgM 
                 PI 153/3 
                 TIB-198 
               
               
                 Glomalin (soil glycoprotein) 
                 mouse 
                 IgM; kappa 
                 32B11 
                 CRL-2559 
               
               
                 Glucocorticoid receptor, mouse and rat 
                 mouse 
                 IgG2b 
                 FIGR 
                 CRL-2173 
               
               
                 Glutamic acid decarboxylase (GAD) 
                 mouse 
                 IgG1 
                 GAD-1 
                 HB-184 
               
               
                 Glycated serum albumin (glycoalbumin) 
                 mouse 
                 IgG 
                 A717 
                 HB-9596 †   
               
               
                 Glycolipid antigen 
                 mouse 
                 IgM 
                 A2B5 clone 105 
                 CRL-1520 
               
               
                 Glycolipids, di- and trifucosylated type 2 chain 
                 mouse 
                 IgG3 
                 FHCR-1-2075/FH4 
                 HB-8775 †   
               
               
                 Glycophorin A, type M 
                 mouse 
                 IgG1; kappa 
                 6A7M 
                 HB-8159 †   
               
               
                 Glycophorin A, type M and type N 
                 mouse 
                 IgG1; kappa 
                 10F7MN 
                 HB-8162 †   
               
               
                 Glycophorin A, type N 
                 mouse 
                 IgG1; kappa 
                 8A2N 
                 HB-8161 †   
               
               
                 Glycophorin A, type N 
                 mouse 
                 IgG2a; kappa 
                 NN-4 
                 HB-8473 †   
               
               
                 Glycophorin A, type N, human 
                 mouse 
                 IgM; kappa 
                 NN-3 
                 HB-8474 †   
               
               
                 Glycophorin A, type N, human 
                 mouse 
                 IgG1; kappa 
                 NN-5 
                 HB-8476 †   
               
               
                 Glycophorin, human 
                 mouse 
                 IgG1 
                 G26.4.1C3/86 
                 HB-9893 †   
               
               
                 Glycoprotein antigen, tumor vascular endothelium 
                 mouse 
                 IgG1 
                 H572 
                 HB-11608 †   
               
               
                 Glycosphingolipid 
                 mouse 
                 IgM 
                 1B2-1B7 
                 TIB-186 
               
               
                 Glycosphingolipid 
                 mouse 
                 IgG3 
                 YI 328-18 
                 HB-9306 †   
               
               
                 Glycosphingolipid, type II chain H structure 
                 mouse 
                 IgM 
                 BE2 
                 TIB-182 
               
               
                 Golgi complex (GCI), rat 
                 mouse 
                 IgG1 
                 6F4C5 
                 CRL-1869 
               
               
                 Golgi vesicular transport protein 
                 mouse 
                 IgM 
                 4A6 
                 CRL-1928 
               
               
                 Gonadotropin releasing hormone, carboxy terminal 
                 mouse 
                 IgG1 
                 USASK/DSIL-LHRH-A1 
                 HB-9094 †   
               
               
                 gp120 glycoprotein 
                 mouse 
                 IgG1 
                 55 
                 HB-9255 †   
               
               
                 gp39, mouse 
                 hamster/mouse 
                 IgG 
                 MR1 
                 CRL-2580 
               
               
                 gp70 envelope antigen (ENV) protein of murine 
                 mouse 
                 IgG2a 
                 48 
                 CRL-1913 
               
               
                 leukemia viruses (MuLV) 
               
               
                 gp70 envelope antigen (ENV) protein of murine 
                 mouse 
                 IgM 
                 514 
                 CRL-1914 
               
               
                 leukemia viruses (MuLV) 
               
               
                 gp90 glycoprotein, human 
                 mouse 
                 IgG2a 
                 G253 
                 HB-9706 †   
               
               
                 GPIIIa, platelet, human 
                 mouse 
                 IgG1 
                 AP-3 
                 HB-242 
               
               
                 Granulocyte macrophage colony stimulating 
                 rat/mouse 
                 IgG2a 
                 BVD2-21C113 
                 HB-9596 †   
               
               
                 factor (GM-CSF), human 
               
               
                 Granulocyte macrophage colony stimulating 
                 rat/mouse 
                 IgG2a 
                 BVD2-23B6.4 
                 HB-9568 †   
               
               
                 factor (GM-CSF), human 
               
               
                 Granulocyte, human 
                 mouse 
                 IgG2b 
                 OKM 1 
                 CRL-8026 
               
               
                 Granulocyte, pig 
                 mouse 
                 IgG1; kappa 
                 74-22-15 
                 HB-142 
               
               
                 Granulocyte, pig 
                 mouse 
                 IgG2b; kappa 
                 74-22-15A 
                 HB-142.1 
               
               
                 Growth hormone (hGH), human 
                 mouse 
                 IgG1; kappa 
                 HGH-B 
                 HB-10596 †   
               
               
                 H-2 (all haplotypes) 
                 rat/mouse 
                 IgG2a 
                 M1/42.3.9.8.HLK 
                 TIB-126 
               
               
                 H-2 b 
                 mouse 
                 IgG1; kappa 
                 B8-24-3 
                 TIB-139 
               
               
                 H-2 D b 
                 mouse 
                 IgM; kappa 
                 23A-5-21S 
                 HB-36 
               
               
                 H-2 D b 
                 mouse 
                 IgM; kappa 
                 28-11-5S 
                 HB-19 
               
               
                 H-2 D d 
                 mouse 
                 IgG2a; kappa 
                 34-2-12S 
                 HB-87 
               
               
                 H-2 D d 
                 mouse 
                 IgG2a; kappa 
                 34-4-20S 
                 HB-75 
               
               
                 H-2 D d 
                 mouse 
                 IgM 
                 34-4-21S 
                 HB-76 
               
               
                 H-2 D d 
                 mouse 
                 IgG2a; kappa 
                 34-5-8S 
                 HB-102 
               
               
                 H-2 D k 
                 mouse 
                 IgG2a; kappa 
                 15-5-5S 
                 HB-24 
               
               
                 H-2 from non-k haplotype mice 
                 rat 
                 IgG2a 
                 K204 
                 HB-221 
               
               
                 H-2 K b 
                 mouse 
                 IgM; kappa 
                 28-13-3S 
                 HB-41 
               
               
                 H-2 K b 
                 mouse 
                 IgG2a 
                 AF6-88.5.3 
                 HB-158 
               
               
                 H-2 K b 
                 mouse 
                 IgG2b; kappa 
                 Y-3 
                 HB-176 
               
               
                 H-2 K b, D b 
                 mouse 
                 IgG2a; kappa 
                 28-8-6S 
                 HB-51 
               
               
                 H-2 K d 
                 mouse 
                 IgM 
                 31-3-4S 
                 HB-77 
               
               
                 H-2 K d 
                 mouse 
                 IgG2a 
                 SF1-1.1.10 
                 HB-159 
               
               
                 H-2 K d, D d 
                 mouse 
                 IgG2a; kappa 
                 34-1-2S 
                 HB-79 
               
               
                 H-2 K d, D d 
                 mouse 
                 IgM 
                 34-7-23S 
                 HB-101 
               
               
                 H-2 K k 
                 mouse 
                 IgG2a; kappa 
                 16-1-11N 
                 HB-16 
               
               
                 H-2 K k 
                 mouse 
                 IgG2a; kappa 
                 16-3-1N 
                 HB-25 
               
               
                 H-2 K k 
                 mouse 
                 IgG2a; kappa 
                 16-3-22S 
                 HB-5 
               
               
                 H-2 K k 
                 mouse 
                 IgG1 
                 AF3-12.1.3 
                 HB-160 
               
               
                 H-2 K k, D k 
                 mouse 
                 IgM; kappa 
                 12-2-2S (clone 5F11) 
                 HB-50 
               
               
                 H-2 K k, D k 
                 mouse 
                 IgG2b; kappa 
                 15-1-5P 
                 HB-53 
               
               
                 H-2 K k, D k 
                 mouse 
                 IgG2a; kappa 
                 15-3-1S 
                 HB-13 
               
               
                 H-2 K k, D k 
                 mouse 
                 IgG2a; kappa 
                 16-1-2N 
                 HB-14 
               
               
                 H-2 K k, D k 
                 mouse 
                 IgG2a; kappa 
                 3-83P 
                 HB-20 
               
               
                 H-2 K of the k, q, p and r haplotypes 
                 mouse 
                 IgG2a 
                 11-4.1 
                 TIB-95 
               
               
                 H7 flagella,  E. coli   
                 mouse 
                 IgG1 
                 MARC 2B7 
                 CRL-2509 †   
               
               
                 Hassall&#39;s bodies, human 
                 mouse 
                 IgM 
                 TE15 
                 HB-206 
               
               
                 Hassall&#39;s bodies, human 
                 mouse 
                 IgG1 
                 TE16 
                 HB-210 
               
               
                 Hassall&#39;s bodies, human 
                 mouse 
                 IgM 
                 TE19 
                 HB-211 
               
               
                 Hassall&#39;s bodies, human 
                 mouse 
                 IgG2a 
                 TE8 
                 HB-212 
               
               
                 Heat-stable antigen, mouse 
                 rat/mouse 
                 IgG2b 
                 M1/69.16.11.HL 
                 TIB-125 
               
               
                 Heat-stable antigen, mouse 
                 rat/mouse 
                 IgG2c 
                 M1/75.16.4.HLK 
                 TIB-127 
               
               
                 HeLA cells 
                 mouse 
                   
                 1A 3   
                 HB-8563 †   
               
               
                 Hematopoietic cells, human 
                 mouse 
                 IgG1 
                 B3/25 
                 CRL-8034 †   
               
               
                 Hepatitis B virus surface antigen (HBsAg) 
                 mouse 
                 IgM 
                 H21F8-1 
                 CRL-8018 †   
               
               
                 Hepatitis B virus surface antigen (HBsAg) 
                 mouse 
                 IgG1 
                 H25B10 
                 CRL-8017 †   
               
               
                 Hepatitis B virus surface antigen (HBsAg) 
                 mouse 
                 IgG1 
                 H25B10 
                 CRL-8017A †   
               
               
                 HER2 receptor 
                 mouse 
                 IgG1 
                 A-HER2 
                 CRL-10463 †   
               
               
                 Herpes simplex virus type 1 (HSV-1) glycoprotein 
                 mouse 
                 IgG2a 
                 52-S 
                 HB-8181 †   
               
               
                 Herpes simplex virus type 1 (HSV-1) glycoprotein 
                 mouse 
                 IgG2a 
                 53-S 
                 HB-8182 †   
               
               
                 Herpes simplex virus type 1 (HSV-1), immediate 
                 mouse 
                 IgG2a 
                 58-S 
                 HB-8183 †   
               
               
                 early protein (ICP 4) 
               
               
                 Herpes simplex virus type 1 (HSV-1), internal capsid 
                 mouse 
                 IgG2a 
                 39-S 
                 HB-8180 †   
               
               
                 protein 8 (ICP 8) 
               
               
                 Herpes simplex virus type 1 (HSV-1), nucleocapsid 
                 mouse 
                 IgG1 
                 1D4 
                 HB-8068 †   
               
               
                 protein (p40) 
               
               
                 Herpes simplex virus type 2 (HSV-2), nucleocapsid 
                 mouse 
                 IgG1 
                 3E1 
                 HB-8067 †   
               
               
                 protein (p40) 
               
               
                 HFE 
                 mouse 
                 IgG1 
                 1C3 
                 CRL-2441 
               
               
                 HFE 
                 mouse 
                 IgG1 
                 2A11 
                 CRL-2442 
               
               
                 HFE 
                 mouse 
                 IgM; kappa 
                 2A5 
                 CRL-2444 
               
               
                 HFE 
                 mouse 
                 IgG1 
                 2B7 
                 CRL-2443 
               
               
                 HFE 
                 mouse 
                 IgM; kappa 
                 3A5 
                 CRL-2440 
               
               
                 HIV gp41 
                 mouse 
                 IgG1; kappa 
                 MH-SVM25 
                 HB-8871 †   
               
               
                 HIV p17 
                 mouse 
                 IgG1; kappa 
                 MH-SVM33C9 
                 HB-8975 †   
               
               
                 HIV p24 
                 mouse 
                 IgG2; kappa 
                 MH-SVM23 
                 HB-8870 †   
               
               
                 HIV p24 
                 mouse 
                 IgG1; kappa 
                 MH-SVM26 
                 HB-8872 †   
               
               
                 HIV-1 gp120 
                 mouse 
                 IgG1 
                 46-2 
                 CRL-2186 
               
               
                 HIV-1 gp120 
                 mouse 
                 IgG1 
                 46-4 
                 CRL-2178 
               
               
                 HIV-1 gp120 
                 mouse 
                 IgG1 
                 46-5 
                 CRL-2184 
               
               
                 HIV-1 gp120 
                 mouse 
                 IgG1 
                 55-2 
                 CRL-2155 
               
               
                 HIV-1 gp120 
                 mouse 
                 IgG1 
                 55-36 
                 CRL-2153 
               
               
                 HIV-1 gp120 
                 mouse 
                 IgG2a 
                 55-6 
                 CRL-2156 
               
               
                 HIV-1 gp120 
                 mouse 
                 IgG2a 
                 55-83 
                 CRL-2185 
               
               
                 HIV-1 gp120 
                 mouse 
                 IgG1 
                 803-15.6 
                 CRL-2395 
               
               
                 HIV-1 p17 
                 mouse 
                 IgG1; kappa 
                 MH-SVM33C9 
                 HB-8975 †   
               
               
                 HIV-1 p24 
                 mouse 
                 IgG1 
                 31-42-19 
                 HB-9726 †   
               
               
                 HIV-1 p24 
                 mouse 
                 IgG1 
                 31-90-25 
                 HB-9725 †   
               
               
                 HLA A2, B17 
                 mouse 
                 IgG1 
                 MA2.1 
                 HB-54 
               
               
                 HLA A3 
                 mouse 
                 IgG2a; kappa 
                 GAP A3 
                 HB-122 
               
               
                 HLA A3 
                 rat/mouse 
                 IgM 
                 WFL3C6.1 
                 HB-8157 †   
               
               
                 HLA B27, B7 
                 mouse 
                 IgG2a 
                 B27M1 
                 HB-157 
               
               
                 HLA B27, Bw47 
                 mouse 
                 IgM 
                 B27M2 
                 HB-165 
               
               
                 HLA B5 
                 mouse 
                 IgG1 
                 4D12 
                 HB-178 
               
               
                 HLA B7 
                 mouse 
                 IgG1 
                 BB7.1 
                 HB-56 
               
               
                 HLA B7, B40 
                 mouse 
                 IgG1 
                 MB 40.3 
                 HB-105 
               
               
                 HLA B7, B40 
                 mouse 
                 IgG1 
                 MB40.2 
                 HB-59 
               
               
                 HLA B7, Bw22, B27 
                 mouse 
                 IgG1 
                 ME 1 
                 HB-119 
               
               
                 HLA B7, B40 
                 mouse 
                 IgG1 
                 BB7.6 
                 HB-115 
               
               
                 HLA Bw6 
                 rat/mouse 
                 IgG2b 
                 SFR8-B6 
                 HB-152 
               
               
                 HLA DC1 
                 mouse 
                 IgG2a 
                 G2a.5 
                 HB-110 
               
               
                 HLA DC1 
                 mouse 
                 IgG2b 
                 G2b.2 
                 HB-109 
               
               
                 HLA DQ 
                 mouse 
                 IgG1; kappa 
                 IVD12 
                 HB-144 
               
               
                 HLA DQw1 
                 mouse 
                 IgG1 
                 Genox 3.53 
                 HB-103 
               
               
                 HLA DR 
                 mouse 
                 IgG1 
                 Antibody 2.06 
                 HB-104 
               
               
                 HLA DR, DP, DQ 
                 mouse 
                 IgG1; kappa 
                 IVA12 
                 HB-145 
               
               
                 HLA DR, DQ 
                 mouse 
                 IgG2a 
                 9.3F10 
                 HB-180 
               
               
                 HLA DR5 
                 rat/mouse 
                 IgG2b 
                 SFR3-DR5 
                 HB-151 
               
               
                 HLA heavy chain 
                 mouse 
                 IgG2a; kappa 
                 171-4 
                 HB-296 
               
               
                 HLA-DR alpha chain 
                 mouse 
                 IgG2a 
                 LB3.1 
                 HB-298 
               
               
                 HMG-CoA reductase (3-hydroxy-3-methyl-glutaryl 
                 mouse 
                 IgG1 
                 A9 
                 CRL-1811 
               
               
                 Coenzyme A reductase) 
               
               
                 Hog renin 
                 mouse 
                 IgG1 
                 F32 VIII C4 
                 CRL-1653 
               
               
                 HTLV-III gp41 
                 mouse 
                 IgG1; kappa 
                 MH-SVM25 
                 HB-8871 †   
               
               
                 HTLV-III p17 
                 mouse 
                 IgG1; kappa 
                 MH-SVM33C9 
                 HB-8975 †   
               
               
                 HTLV-III p24 
                 mouse 
                 IgG2; kappa 
                 MH-SVM23 
                 HB-8870 †   
               
               
                 HTLV-III p24 
                 mouse 
                 IgG1; kappa 
                 MH-SVM26 
                 HB-8872 †   
               
               
                 Human epidermal growth factor (EGF) receptor 
                 mouse 
                 IgG2a; kappa 
                 Mab 108 
                 HB-9764 †   
               
               
                 H-Y antigen 
                 mouse 
                 IgM; kappa 
                 12/44 
                 HB-9070 †   
               
               
                 H-Y antigen 
                 mouse 
                 IgM; kappa 
                 12/49 
                 HB-9071 †   
               
               
                 I-A 
                 mouse 
                 IgG2a 
                 Y-3P 
                 HB-183 
               
               
                 I-A b 
                 mouse 
                 IgM 
                 25-5-16S 
                 HB-37 
               
               
                 I-A b 
                 mouse 
                 IgM 
                 25-9-3S 
                 HB-38 
               
               
                 I-A b 
                 mouse 
                 IgG2a 
                 AF6-120-1.2 
                 HB-163 
               
               
                 I-A b and I-A d 
                 mouse 
                 IgG2a; kappa 
                 25-9-17S II 
                 HB-26 
               
               
                 I-A b and I-A d 
                 mouse 
                 IgG3 
                 BP107.2.2 
                 TIB-154 
               
               
                 I-A b, I-A d 
                 mouse 
                 IgM; kappa 
                 28-16-8S 
                 HB-35 
               
               
                 I-A b, I-A d, I-A q, I-E d and I-E k 
                 rat/mouse 
                 IgG2b; kappa 
                 M5/114.15.2 
                 TIB-120 
               
               
                 I-A b, d 
                 rat/mouse 
                 IgG2b 
                 B21-2 
                 TIB-229 
               
               
                 I-A d 
                 mouse 
                 IgG2a; kappa 
                 34-5-3S 
                 HB-85 
               
               
                 I-A d 
                 mouse 
                 IgG2a; kappa 
                 MK-D6 
                 HB-3 
               
               
                 I-A k 
                 mouse 
                 IgG2b 
                 11-5.2.1.9 
                 TIB-94 
               
               
                 I-A k 
                 mouse 
                 IgM; kappa 
                 26-7-11S 
                 HB-15 
               
               
                 I-A k 
                 mouse 
                 IgM 
                 26-8-16S 
                 HB-42 
               
               
                 I-A of k, r, f and s haplotypes 
                 mouse 
                 IgG2a 
                 10-3.6.2 
                 TIB-92 
               
               
                 I-A of the k, r, f and s haplotypes 
                 mouse 
                 IgG2b 
                 10-2.16 
                 TIB-93 
               
               
                 I-A s 
                 mouse 
                 IgG2b 
                 MK-S4 
                 HB-4 
               
               
                 Ia, human 
                 mouse 
                 IgG1; kappa 
                 L203 
                 HB-171 
               
               
                 Ia, human 
                 mouse 
                 IgG1; kappa 
                 L227 
                 HB-96 
               
               
                 Ia, human 
                 mouse 
                 IgG2a 
                 L243 
                 HB-55 
               
               
                 I-A, I-E, monomorphic, mouse 
                 hamster/mouse 
                 IgG 
                 N22 
                 HB-225 
               
               
                 Ia, rabbit 
                 mouse 
                 IgG2a 
                 2C4 
                 CRL-1760 
               
               
                 ICAM-1, canine 
                 mouse 
                 IgG1 
                 CL18/6 
                 CRL-2518 
               
               
                 ICAM-1, human 
                 mouse 
                 IgG2a 
                 R6.5.D6.E9.B2 
                 HB-9580 †   
               
               
                 ICAM-1, mouse 
                 rat 
                 IgG2b 
                 YN1/1.7.4 
                 CRL-1878 
               
               
                 ICAM-1, mouse 
                 rat/mouse 
                 IgG2a 
                 BE29G1 
                 HB-233 
               
               
                 ICAM-4, rat 
                 mouse 
                 IgG1 
                 127H 
                 HB-11911 †   
               
               
                 Idiotypic determinant on on anti-chlamydia genus 
                 mouse 
                 IgG1; kappa 
                 91MS441 
                 HB-11301 †   
               
               
                 antibody 
               
               
                 Idiotypic determinant on the P3X63Ag8 myeloma 
                 mouse protein 
                 IgG2b; kappa 
                 80 V 5B4 
                 TIB-132 
               
               
                 I-E 
                 mouse 
                 IgG2b 
                 Y-17 
                 HB-179 
               
               
                 I-E k 
                 mouse 
                 IgG2a; kappa 
                 14-4-4S 
                 HB-32 
               
               
                 I-E k 
                 mouse 
                 IgG2a; kappa 
                 17-3-3S 
                 HB-6 
               
               
                 Ig-4a allotype on mouse IgG1 
                 mouse 
                 IgG2a 
                 Ig(4a)10.9 
                 HB-146 
               
               
                 IgA, human 
                 mouse 
                 IgG1; kappa 
                 CH-EB6 
                 HB-200 
               
               
                 IgD, Ig-5a allotype, mouse 
                 mouse 
                 IgG2a 
                 Ig(5a)7.2 (formerly 10- 
                 TIB-149 
               
               
                   
                   
                   
                 4-22) 
               
               
                 IgD, mouse 
                 rat/mouse 
                 IgG2a 
                 11-26c 
                 HB-250 
               
               
                 IgE, human 
                 mouse 
                 IgG1; kappa 
                 CIA-E-4.15 
                 HB-235 
               
               
                 IgE, human 
                 mouse 
                 IgG1; kappa 
                 CIA-E-7.12 
                 HB-236 
               
               
                 IgE, human 
                 mouse 
                 IgG2a; kappa 
                 E5BB3IIA2 
                 HB-121 
               
               
                 IgG (Fc), human 
                 mouse 
                 IgG2b 
                 HP6000 
                 CRL-1754 
               
               
                 IgG (Fc), human 
                 mouse 
                 IgG2a 
                 HP6017 
                 CRL-1753 
               
               
                 IgG (Fc), human 
                 mouse 
                 IgG1 
                 HP605B 
                 CRL-1786 
               
               
                 IgG (Fd, F(ab′)2, Fab), human 
                 mouse 
                 IgG2a 
                 HP6045 
                 CRL-1757 
               
               
                 IgG Fc receptor, human 
                 mouse 
                 IgG2b 
                 IV.3 
                 HB-217 
               
               
                 IgG1 (Fc), human 
                 mouse 
                 IgG2b 
                 HP6001 
                 CRL-1755 
               
               
                 IgG1 (Fc), rat 
                 mouse 
                 IgG2b 
                 RG11/39.4 
                 TIB-170 
               
               
                 IgG2 (Fc), human 
                 mouse 
                 IgG1 
                 HP6002 
                 CRL-1788 
               
               
                 IgG2 (Fd), human 
                 mouse 
                 IgG1 
                 HP6014 
                 CRL-1752 
               
               
                 IgG2, mouse 
                 rat/mouse 
                 IgG2b; kappa 
                 7D2-1.4.1.5 
                 HB-92 
               
               
                 IgG2, mouse 
                 rat/mouse 
                 IgG2a 
                 ED1-19-1-6-5 
                 HB-90 
               
               
                 IgG2a (Fab′), rat 
                 mouse 
                 IgG2b 
                 RG9/6.13 HLK 
                 TIB-167 
               
               
                 IgG2a (Fc), rat 
                 mouse 
                 IgG2b 
                 RG7/1.30 
                 TIB-173 
               
               
                 IgG2a Ig-1a allotype, mouse 
                 mouse 
                 IgG2a 
                 Ig(1a)8.3 (formerly 20-8.3) 
                 TIB-148 
               
               
                 IgG2a, Igh-1b allotype, mouse 
                 mouse 
                 IgM 
                 RDP 45/20 
                 TIB-98 
               
               
                 IgG2b (Fc), rat 
                 mouse 
                 IgG2b 
                 RG7/11.1 
                 TIB-174 
               
               
                 IgG3 (Fc), human 
                 mouse 
                 IgG1 
                 HP6003 
                 CRL-1756 
               
               
                 IgG3 (hinge), human 
                 mouse 
                 IgG1 
                 HP6047 
                 CRL-1774 
               
               
                 IgG3 hinge region, human 
                 mouse 
                 IgG1 
                 HP6050 
                 CRL-1768 
               
               
                 IgG3, mouse 
                 rat/mouse 
                 IgG1 
                 2E.6 
                 HB-128 
               
               
                 IgG4 (Fab), human 
                 mouse 
                 IgG2a 
                 HP6020 
                 CRL-1789 
               
               
                 IgG4 (Fc), human 
                 mouse 
                 IgG3 
                 HP6023 
                 CRL-1776 
               
               
                 IgG4 (Fc), human 
                 mouse 
                 IgG1 
                 HP6025 
                 CRL-1775 
               
               
                 Igh-5.3 (IgD b, e) 
                 mouse 
                 IgG1 
                 AF6-122.2.5 
                 HB-199 
               
               
                 Igh-5.4 (IgD a) 
                 mouse 
                 IgG2b 
                 AMS 9.1.1.1 
                 HB-161 
               
               
                 Igh-5.5 (IgD e) 
                 mouse 
                 IgG2b 
                 AF4-73.3.1 
                 HB-201 
               
               
                 Igh-5b (IgD b allotype) 
                 mouse 
                 IgG1 
                 Ig(5b)6.3 
                 TIB-96 
               
               
                 Igh-6.6 (IgM b) 
                 mouse 
                 IgG1 
                 AF6-78.25.4 
                 HB-162 
               
               
                 IgM (Igh-6.5 allotype), mouse 
                 rat/mouse 
                 IgG1; kappa 
                 Bet-1 
                 HB-100 
               
               
                 IgM (lambda), human 
                 mouse 
                 IgG2b; kappa 
                 LP4.4 
                 HB-232 
               
               
                 IgM (mu heavy chain), mouse 
                 rat/mouse 
                 IgG2b 
                 331.12 
                 TIB-129 
               
               
                 IgM, bovine 
                 mouse 
                 IgG1 
                 IL-A30 
                 CRL-1894 
               
               
                 IgM, human 
                 mouse 
                 IgG1 
                 M-2E6 
                 HB-138 
               
               
                 IgM, mouse 
                 rat/mouse 
                 IgG1; kappa 
                 Bet-2 
                 HB-88 
               
               
                 IgM, pig 
                 mouse 
                 IgG1; kappa 
                 5C9 
                 HB-8371 †   
               
               
                 IgM, rabbit 
                 mouse 
                 IgG1 
                 NRbM 
                 CRL-1839 
               
               
                 IL-1 beta, human 
                 mouse 
                 IgG1; kappa 
                 ILB1-H21 
                 HB-10220 †   
               
               
                 IL-1 beta, human 
                 mouse 
                 IgG1; kappa 
                 ILB1-H34 
                 HB-10221 †   
               
               
                 IL-1 beta, human 
                 mouse 
                 IgG1; kappa 
                 ILB1-H6 
                 HB-10219 †   
               
               
                 IL-1 beta, human 
                 mouse 
                 IgG2b; kappa 
                 ILB1-H67 
                 HB-10222 †   
               
               
                 IL-1 beta, recombinant, bovine 
                 mouse 
                 IgG1 
                 SA22 
                 CRL-2052 
               
               
                 IL-2, mouse 
                 rat/mouse 
                 IgG2a 
                 S4B6-1 
                 HB-10968 †   
               
               
                 IL-4, mouse 
                 rat/mouse 
                 IgG1 
                 11B11 
                 HB-188 
               
               
                 IL-8, human 
                 mouse 
                 IgG1 
                 EL-NC-1S 
                 HB-9647 †   
               
               
                 IL-12 p40, mouse 
                 rat/mouse 
                 IgG2a 
                 R1-5D9 
                 CRL-2360 
               
               
                 IL-12 p40, mouse 
                 rat/mouse 
                 IgG2b 
                 R2-10F6 
                 CRL-2358 
               
               
                 IL-12 p75, mouse 
                 rat/mouse 
                 IgG2b 
                 R2-9A5 
                 CRL-2357 
               
               
                 IL-12, human 
                 rat/mouse 
                 IgG1 
                 20C2 
                 CRL-2382 
               
               
                 IL-15, human 
                 mouse 
                 IgG1 
                 hIL-15-M110 
                 HB-12061 †   
               
               
                 IL-15, human 
                 mouse 
                 IgG1 
                 hIL-15-M111 
                 HB-12062 †   
               
               
                 Infectious bovine rhinotracheitis virus (IBRV) 
                 mouse 
                 IgG1 
                 1B8-F11 
                 CRL-1852 
               
               
                 Infectious bursal disease (IBD) virus 
                 mouse 
                 IgG2a; kappa 
                 B69 
                 HB-9437 †   
               
               
                 Infectious bursal disease (IBD) virus 
                 mouse 
                 IgG2a; kappa 
                 R63 
                 HB-9490 †   
               
               
                 Infectious bursal disease (IBD) virus, serotypes 
                 mouse 
                 IgG1; kappa 
                 B29 
                 HB-9746 †   
               
               
                 1 and 2 
               
               
                 Inflammatory cells, human 
                 mouse 
                 IgG1 
                 MY904 
                 HB-9510 †   
               
               
                 Influenzavirus A matrix protein (M) 
                 mouse 
                 IgG1 
                 M2-1C6-4R3 
                 HB-64 
               
               
                 Influenzavirus A nucleoprotein 
                 mouse 
                 IgG1 
                 46/4 
                 HB-67 
               
               
                 Influenzavirus A nucleoprotein 
                 mouse 
                 IgG2a 
                 H16-L10-4RS 
                 HB-65 
               
               
                 Influenzavirus hemagglutinin 
                 mouse 
                 IgG2a 
                 73/1 
                 HB-66 
               
               
                 Insulin 
                 mouse 
                 IgG2a; kappa 
                 BE3F9 
                 HB-133 
               
               
                 Insulin 
                 mouse 
                 IgG1; kappa 
                 CC9C10 
                 HB-123 
               
               
                 Insulin 
                 mouse 
                 IgG1; kappa 
                 CE9H9 
                 HB-127 
               
               
                 Insulin 
                 mouse 
                 IgG1; kappa 
                 CG7C7 
                 HB-126 
               
               
                 Insulin 
                 mouse 
                 IgG2a; kappa 
                 DB9G8 
                 HB-124 
               
               
                 Insulin (residues A8-10), human 
                 mouse 
                 IgG1; kappa 
                 AE9D6 
                 HB-125 
               
               
                 Insulin receptor, human 
                 mouse 
                 IgG1; kappa 
                 αIR-1 
                 HB-175 
               
               
                 Insulin receptor, placental, human 
                 mouse 
                 IgG1 
                 DII 33.1 
                 CRL-1827 
               
               
                 Integrin, alpha 1, human 
                 mouse 
                 IgG1 
                 TS2/7.1.1 
                 HB-245 
               
               
                 Integrin, alpha 4, sheep 
                 mouse 
                 IgG2b 
                 FW3-218-1 
                 HB-261 
               
               
                 Integrin, alpha 4/beta 7, mouse 
                 rat/mouse 
                 IgG2a 
                 DATK32 
                 HB-294 
               
               
                 Integrin, beta 1 subunit, mouse 
                 rat/mouse 
                 IgG2a 
                 KMI6 
                 CRL-2179 
               
               
                 Integrin, beta 1, human 
                 mouse 
                 IgG1 
                 TS2/16.2.1 
                 NB-243 
               
               
                 Integrin, beta 1, sheep 
                 mouse 
                 IgG1 
                 FW4-101-1-1 
                 HB-289 
               
               
                 Integrin, beta 2, mouse 
                 hamster/mouse 
                 IgG 
                 2E6 
                 HB-226 
               
               
                 Integrin, beta 3, human 
                 mouse 
                 IgG1 
                 AP-3 
                 HB-242 
               
               
                 Integrin, beta 7 Integrin, mouse 
                 rat/mouse 
                 IgG2a 
                 FIB21 
                 HB-295 
               
               
                 Integrin, beta 7 Integrin, mouse 
                 rat/mouse 
                 IgG2a 
                 FIB504.64 
                 HB-293 
               
               
                 Integrin, leukocyte, mouse 
                 hamster/mouse 
                 IgG 
                 N418 
                 HB-224 
               
               
                 Integrin-associated protein, human 
                 mouse 
                 IgG1 
                 B6H12.2 
                 HB-9771 †   
               
               
                 Integrin-like cellular adhesion molecule 
                 rat/mouse 
                 IgG2b 
                 PS/2 
                 CRL-1911 
               
               
                 Intercellular adhesion molecule 1, mouse 
                 rat 
                 IgG2b 
                 YN1/1.7.4 
                 CRL-1878 
               
               
                 Intercellular adhesion molecule 1, mouse 
                 rat/mouse 
                 IgG2a 
                 BE29G1 
                 HB-233 
               
               
                 Interferon gamma receptor, mouse 
                 rat/mouse 
                 IgA; kappa 
                 GR-96 
                 CRL-2013 
               
               
                 Interferon gamma receptor, mouse 
                 rat/mouse 
                 IgG2a; kappa 
                 GR-20 
                 CRL-2024 
               
               
                 Interferon, gamma, human 
                 mouse 
                 IgG1 
                 γ3-11.1 
                 HB-8700 †   
               
               
                 Interferon, gamma, human 
                 mouse 
                 IgG1 
                 IFGCP-F1BA10 
                 HB-8291 †   
               
               
                 Interferon, gamma, mouse 
                 rat/mouse 
                 IgG1 
                 R4-6A2 
                 HB-170 
               
               
                 Interleukin 1 beta, human 
                 mouse 
                 IgG1; kappa 
                 ILB1-H21 
                 HB-10220 †   
               
               
                 Interleukin 1 beta, human 
                 mouse 
                 IgG1; kappa 
                 ILB1-H34 
                 HB-10221 †   
               
               
                 Interleukin 1 beta, human 
                 mouse 
                 IgG1; kappa 
                 ILB1-H6 
                 HB-10219 †   
               
               
                 Interleukin 1 beta, human 
                 mouse 
                 IgG2b; kappa 
                 ILB1-H67 
                 HB-10222 †   
               
               
                 Interleukin 1 beta, recombinant, bovine 
                 mouse 
                 IgG1 
                 SA22 
                 CRL-2052 
               
               
                 Interleukin 15, human 
                 mouse 
                 IgG1 
                 hIL-15-M110 
                 HB-12061 †   
               
               
                 Interleukin 15, human 
                 mouse 
                 IgG1 
                 hIL-15-M111 
                 HB-12062 †   
               
               
                 Interleukin 2 receptor, human 
                 mouse 
                 IgG1 
                 2A3A1H 
                 HB-8555 †   
               
               
                 Interleukin 2 receptor, human 
                 mouse 
                 IgG2a 
                 7G7B6 
                 HB-8784 †   
               
               
                 Interleukin 2 receptor, mouse 
                 rat/mouse 
                 IgM; kappa 
                 7D4 
                 CRL-1698 
               
               
                 Interleukin 2 receptor, mouse 
                 rat/mouse 
                 IgG1 
                 PC 61 5.3 
                 TIB-222 
               
               
                 Interleukin 2, mouse 
                 rat/mouse 
                 IgG2a 
                 S4B6-1 
                 HB-10968 †   
               
               
                 Interleukin 8 receptor type B (IL-8R-B), human 
                 mouse 
                 IgG2a 
                 10H2.12.1 
                 HB-11494 †   
               
               
                 Interleukin 8 receptor type B (IL-8R-B), human 
                 mouse 
                 IgG2a 
                 4D1.5.7 
                 HB-11495 †   
               
               
                 Interleukin 8, human 
                 mouse 
                 IgG1 
                 EL-NC-1S 
                 HB-9647 †   
               
               
                 Intermediate filaments 
                 mouse 
                 IgG1 
                 α Intermediate Filament 
                 TIB-131 
               
               
                 Intracellular adhesion molecule 1 (ICAM-1), canine 
                 mouse 
                 IgG1 
                 CL18/6 
                 CRL-2518 
               
               
                 Intracellular adhesion molecule 1 (ICAM-1), human 
                 mouse 
                 IgG2a 
                 R6.5.D6.E9.B2 
                 HB-9580 †   
               
               
                 Intracellular adhesion molecule 1 (ICAM-1), mouse 
                 rat 
                 IgG2b 
                 YN1/1.7.4 
                 CRL-1878 
               
               
                 Intracellular adhesion molecule 1 (ICAM-1), mouse 
                 rat/mouse 
                 IgG2a 
                 BE29G1 
                 HB-233 
               
               
                 Intracellular adhesion molecule 1 (ICAM-1), rat 
                 mouse 
                 IgG1 
                 127H 
                 HB-11911 †   
               
               
                 Invected protein,  Drosophila melanogaster   
                 mouse 
                 IgG1 
                 4D9D4 
                 CRL-1818 
               
               
                 J5 endotoxin core,  Escherichia coli   
                 mouse 
                 IgG1 
                 J5-1 
                 HB-8297 †   
               
               
                 J5 endotoxin core,  Escherichia coli   
                 mouse 
                 IgG1 
                 J5-2 
                 HB-8298 †   
               
               
                 K99 pilus,  Escherichia coli   
                 mouse 
                 IgG1; kappa 
                 2BD4E4 K99 
                 HB-8178 †   
               
               
                 Kappa light chain (monotypic determinant), rat 
                 mouse 
                 IgG2b 
                 RG7/9.1 HLK 
                 TIB-169 
               
               
                 Kappa light chain (RI-1a and RI-1b allotypes), rat 
                 mouse 
                 IgG2a; kappa 
                 MAR 18.5 
                 TIB-216 
               
               
                 Kappa light chain [kappa 1b (LEW)], rat 
                 mouse 
                 IgG2a 
                 RG7/7.6 HL 
                 TIB-172 
               
               
                 Kappa light chain, human 
                 mouse 
                 IgG1; kappa 
                 141PF11 
                 HB-45 
               
               
                 Kappa light chain, human 
                 mouse 
                 IgG1 
                 HP6053 
                 CRL-1758 
               
               
                 Kappa light chain, human 
                 mouse 
                 IgG1; kappa 
                 TB 28-2 
                 HB-61 
               
               
                 Kappa light chain, mouse 
                 rat/mouse 
                 IgG1 
                 187.1 
                 HB-58 
               
               
                 Kidney tubules, human 
                 mouse 
                 IgG1 
                 DAL K20 
                 CRL-2288 
               
               
                 Kidney tubules, human 
                 mouse 
                 IgG1 
                 DAL K29 
                 CRL-2291 
               
               
                 Kininogen heavy chain, human 
                 mouse 
                 IgG1 
                 2B5 
                 HB-8963 †   
               
               
                 Kininogen light chain, human 
                 mouse 
                 IgG1 
                 C11C1 
                 HB-8964 †   
               
               
                 Kunitz soybean trypsin inhibitor 
                 mouse 
                 IgG1; kappa 
                 C129 
                 HB-9516 †   
               
               
                 Kunitz soybean trypsin inhibitor 
                 mouse 
                 IgG1; kappa 
                 C171 
                 HB-9515 †   
               
               
                 Kunitz soybean trypsin inhibitor 
                 mouse 
                 IgG1; kappa 
                 C180 
                 HB-9517 †   
               
               
                 L d, D b and D q 
                 mouse 
                 IgG2a; kappa 
                 28-14-8S 
                 HB-27 
               
               
                 L d, D q, L q and L b 
                 mouse 
                 IgG2a; kappa 
                 30-5-7S 
                 HB-31 
               
               
                 L3T4 antigen (T cell), mouse 
                 rat/mouse 
                 IgG2b 
                 GK1.5 
                 TIB-207 
               
               
                 La Crosse Virus, G1 envelope glycoprotein 
                 mouse 
                 IgG2b 
                 807.15 
                 CRL-2287 
               
               
                 La Crosse Virus, G1 envelope glycoprotein 
                 mouse 
                 IgG1 
                 807.31 
                 CRL-2282 
               
               
                 La Crosse Virus, G1 envelope glycoprotein 
                 mouse 
                 IgG2a 
                 807.33 
                 CRL-2290 
               
               
                 La/SSB, bovine 
                 mouse 
                 IgG 
                 La1 
                 HB-8609 †   
               
               
                 LAM-1, human 
                 mouse 
                 IgG1 
                 DREG200 
                 HB-302 
               
               
                 LAM-1, human 
                 mouse 
                 IgG1 
                 DREG56 
                 HB-300 
               
               
                 Lambda 1 light chain, mouse 
                 mouse 
                 IgG1; kappa 
                 LS-136 
                 TIB-157 
               
               
                 Lambda light chain, human 
                 mouse 
                 IgG2a 
                 HP6054 
                 CRL-1763 
               
               
                 Laminin 
                 rat/mouse 
                 IgG2b 
                 2AB1-IA10 
                 HB-8210 †   
               
               
                 LECAM, human 
                 mouse 
                 IgG1 
                 DREG200 
                 HB-302 
               
               
                 LECAM, human 
                 mouse 
                 IgG1 
                 DREG56 
                 HB-300 
               
               
                 
                   Legionella pneumophila 
                 
                 mouse 
                 IgG2a 
                 LP3IIG2 
                 HB-8472 †   
               
               
                   Legionella pneumophila  serogroup 1 
                 mouse 
                 IgG3 
                 Lp1 MAB 1 
                 CRL-1765 
               
               
                   Legionella pneumophila  serogroup 1 
                 mouse 
                 IgG2b 
                 Lp1 MAB 2 
                 CRL-1770 
               
               
                   Legionella pneumophila  serogroup 1 
                 mouse 
                 IgG2b 
                 Lp1 MAB 3 
                 CRL-1767 
               
               
                 Leptomeningeal cell, rat neural antigen-2, RAN-2) 
                 mouse 
                 IgG2a 
                 Ran-2 
                 TIB-119 
               
               
                 Leptospira pomona type kennewicki 
                 mouse 
                 IgA 
                 2D7F10 
                 CRL-2025 
               
               
                 Leu 200 glycoproteins, human 
                 mouse 
                 IgG2a; kappa 
                 4C 
                 HB-8311 †   
               
               
                 Leu-5 
                 mouse 
                 IgM 
                 TM1 
                 HB-169 
               
               
                 Leu8, human 
                 mouse 
                 IgG1 
                 DREG200 
                 HB-302 
               
               
                 Leu8, human 
                 mouse 
                 IgG1 
                 DREG56 
                 HB-300 
               
               
                 Leu8, mouse 
                 rat/mouse 
                 IgG2a 
                 MEL-14 
                 HB-132 
               
               
                 Leukocyte common antigen, human 
                 mouse 
                 IgG2a; kappa 
                 GAP 8.3 
                 HB-12 
               
               
                 Leukocyte function antigen 1, alpha subunit, mouse 
                 rat/mouse 
                 IgG2a; kappa 
                 M17/4.4.11.9 (new 
                 TIB-217 
               
               
                   
                   
                   
                 clone of M17/4.2) 
               
               
                 Leukocyte function antigen 1, mouse 
                 rat/mouse 
                 IgG2b 
                 FD441.8 
                 TIB-213 
               
               
                 LEW RT1.A 
                 rat/mouse 
                 IgM 
                 WFL3C6.1 
                 HB-8157 †   
               
               
                 LEW RT1.A 
                 rat/mouse 
                 IgM 
                 WFL4F12.3 
                 HB-8156 †   
               
               
                 Lewis a and b blood group antigens, human 
                 mouse 
                 IgG3 
                 151-5-G2-12 
                 HB-8322 †   
               
               
                 Lewis a and b blood group antigens, human 
                 mouse 
                 IgG3 
                 151-5-G3-5 
                 HB-8323 †   
               
               
                 Lewis a antigen 
                 mouse 
                 IgG1 
                 BC9-E5 
                 CRL-1670 
               
               
                 Lewis a antigen 
                 mouse 
                 IgG2a; kappa 
                 CA3-F4 
                 CRL-1667 
               
               
                 Lewis a antigen 
                 mouse 
                 IgG1; kappa 
                 CF4-C4 
                 CRL-1716 
               
               
                 Lewis a blood group antigen, human 
                 mouse 
                 IgG3 
                 151-6-A7-9 
                 HB-8324 †   
               
               
                 Lewis b blood group antigen, human 
                 mouse 
                 IgG1 
                 130-3-F7-5 
                 HB-8326 †   
               
               
                 Lewis b blood group antigen, human 
                 mouse 
                 IgM 
                 143-2-A6-11 
                 HB-8325 †   
               
               
                 Lex (tumor-associated fucoganglioside) 
                 mouse 
                 IgM 
                 FHCR-1-2624/FH6/ 
                 HB-8873 †   
               
               
                   
                   
                   
                 FHOT-1-3019 
               
               
                 LFA-1, beta subunit, mouse 
                 rat/mouse 
                 IgG2a; kappa 
                 M18/2.a.12.7 (new 
                 TIB-218 
               
               
                   
                   
                   
                 clone of M18/2.a.8) 
               
               
                 LFA-1, mouse 
                 rat/mouse 
                 IgG2b 
                 FD441.8 
                 TIB-213 
               
               
                 LFA-1, mouse 
                 rat/mouse 
                 IgG2a; kappa 
                 M17/4.4.11.9 (new 
                 TIB-217 
               
               
                   
                   
                   
                 clone of M17/4.2) 
               
               
                 LFA-1, mouse 
                 rat/mouse 
                 IgG2b; kappa 
                 M17/5.2 
                 TIB-237 
               
               
                 LGL-1 
                 rat/mouse 
                 IgG2a 
                 4D11 
                 HB-240 
               
               
                 Lgp100a 
                 rat/mouse 
                 IgG2a 
                 30-C7 
                 TIB-106 
               
               
                 Lipopolysaccharide,  chlamydia   
                 mouse 
                 IgG3 
                 L2I-6 
                 HB-8705 †   
               
               
                 Lipoprotein H2,  Pseudomonas aeruginosa   
                 mouse 
                 IgG1 
                 MA1-6 
                 CRL-1783 
               
               
                 Lipoprotein receptor related protein (LRP), 
                 mouse 
                 IgG1 
                 IgG-5D7 
                 CRL-1938 
               
               
                 515-kDa subunit, rabbit 
               
               
                 Lipoprotein receptor related protein (LRP), 
                 mouse 
                 IgG1 
                 IgG-1B3 
                 CRL-1937 
               
               
                 85-kDa subunit, rabbit 
               
               
                 Lipoprotein receptor related protein (LRP), carboxy 
                 mouse 
                 IgG1 
                 IgG-11H4 
                 CRL-1936 
               
               
                 terminal 
               
               
                 Low density lipoprotein (LDL) receptor, bovine 
                 mouse 
                 IgG1 
                 9D9 
                 CRL-1703 
               
               
                 Low density lipoprotein (LDL) receptor, bovine 
                 mouse 
                 IgG2b 
                 C7 
                 CRL-1691 
               
               
                 Low density lipoprotein (LDL) receptor, human 
                 mouse 
                 IgG1 
                 IgG-4A4 
                 CRL-1898 
               
               
                 Low density lipoprotein (LDL), human 
                 mouse 
                 IgG1 
                 B1B3 
                 CRL-2249 
               
               
                 Low density lipoprotein (LDL), human 
                 mouse 
                 IgG1 
                 B1B6 
                 CRL-2248 
               
               
                 LPAM-1, mouse 
                 rat/mouse 
                 IgG2b 
                 R1-2 
                 HB-227 
               
               
                 Lung cancer 
                 mouse 
                 IgG1 
                 L18 
                 HB-8628 †   
               
               
                 Lung cancer 
                 mouse 
                 IgM 
                 L5 
                 HB-8627 †   
               
               
                 Lung cancer, human 
                 mouse 
                 IgG2a; kappa 
                 703D4 
                 HB-8301 †   
               
               
                 Luteinizing hormone releasing hormone (LHRH), 
                 mouse 
                 IgG1 
                 USASK/DSIL-LHRH-A1 
                 HB-9094 †   
               
               
                 carboxy terminal 
               
               
                 Luteinizing hormone/chorionic gonadotropin 
                 mouse 
                 IgG2a 
                 FSHR-323 
                 CRL-2689 
               
               
                 (LH/hCG) receptor, human 
               
               
                 Luteinizing hormone/chorionic gonadotropin 
                 mouse 
                 IgG1 
                 LHR-1055 
                 CRL-2687 
               
               
                 (LH/hCG) receptor, human 
               
               
                 Luteinizing hormone/chorionic gonadotropin 
                 mouse 
                 IgG1 
                 LHR-29 
                 CRL-2685 
               
               
                 (LH/hCG) receptor, human 
               
               
                 Luteinizing hormone/chorionic gonadotropin 
                 mouse 
                 IgG1 
                 LHR-74 
                 CRL-2686 
               
               
                 (LH/hCG) receptor, human 
               
               
                 Ly 6.2C, mouse 
                 mouse 
                 IgG1 
                 143-4.2 
                 CRL-1970 
               
               
                 Lyb 2.1, mouse 
                 mouse 
                 IgG2b 
                 10-1.D.2 
                 TIB-165 
               
               
                 Lyb 8.2, mouse 
                 mouse 
                 IgG1 
                 Cy34.1.2 
                 TIB-163 
               
               
                 Lymphocyte function antigen 1 (LFA-1) alpha 
                 mouse 
                 IgG1 
                 TS1/22.1.1.13 
                 HB-202 
               
               
                 subunit, human 
               
               
                 Lymphocyte function antigen 1 (LFA-1) beta 
                 mouse 
                 IgG1 
                 TS1/18.1.2.11 
                 HB-203 
               
               
                 subunit, human 
               
               
                 Lymphocyte function antigen 1 (LFA-1), human 
                 mouse 
                 IgG1 
                 TS2/4.1.1 
                 HB-244 
               
               
                 Lymphocyte function antigen 1, mouse 
                 rat/mouse 
                 IgG2b; kappa 
                 M17/5.2 
                 TIB-237 
               
               
                 Lymphocyte function antigen 2 (LFA-2), human 
                 mouse 
                 IgG1 
                 TS2/18.1.1 
                 HB-195 
               
               
                 Lymphocyte function antigen 3 (LFA-3), human 
                 mouse 
                 IgG1 
                 TS2/9.1.4.3 
                 HB-205 
               
               
                 Lymphocyte Peyer&#39;s patch HEV adhesion molecule, 
                 rat/mouse 
                 IgG2b 
                 R1-2 
                 HB-227 
               
               
                 mouse 
               
               
                 Lymphocyte surface receptor for endothelium, 
                 rat/mouse 
                 IgG2a 
                 MEL-14 
                 HB-132 
               
               
                 mouse 
               
               
                 Lymphocyte, mouse 
                 rat/mouse 
                 IgM 
                 GL7 
                 HB-254 
               
               
                 Lymphoma cells, canine 
                 mouse 
                 IgG2a 
                 Hybridoma 231 
                 HB-9401 †   
               
               
                 Lymphoma cells, canine 
                 mouse 
                 IgG1 
                 Hybridoma 234 
                 HB-9402 †   
               
               
                 Lymphoma cells, canine 
                 mouse 
                 IgG2a 
                 Hybridoma 234 s.2a 
                 HB-9403 †   
               
               
                 Lyt 2.2, mouse 
                 mouse 
                 IgM 
                 83-12-5 
                 CRL-1971 
               
               
                 Lyt-1 (all alleles), mouse 
                 rat/mouse 
                 IgG2a 
                 53-7.313 
                 TIB-104 
               
               
                 Lyt-2 (all alleles), mouse 
                 rat/mouse 
                 IgM 
                 3.15S 
                 TIB-211 
               
               
                 Lyt-2 (all alleles), mouse 
                 rat/mouse 
                 IgG2a 
                 53-6.72 
                 TIB-105 
               
               
                 Lyt-2.1, mouse 
                 mouse 
                 IgG2a 
                 116-13.1 
                 HB-129 
               
               
                 Lyt-2.2, mouse 
                 mouse 
                 IgM 
                 41-3.48 
                 HB-130 
               
               
                 Lyt-2.2, mouse 
                 mouse 
                 IgM 
                 HO-2.2 
                 TIB-150 
               
               
                 Lyt-2.2, mouse 
                 rat/mouse 
                 IgG2b 
                 2.43 
                 TIB-210 
               
               
                 Mac-1, alpha chain, mouse 
                 rat/mouse 
                 IgG2b 
                 M1/70.15.11.5.HL 
                 TIB-128 
               
               
                 Mac-1, beta subunit, mouse 
                 rat/mouse 
                 IgG2a; kappa 
                 M18/2.a.12.7 (new 
                 TIB-218 
               
               
                   
                   
                   
                 clone of M18/2.a.8) 
               
               
                 Mac-1, human 
                 mouse 
                 IgG1 
                 LM2/1.6.11 
                 HB-204 
               
               
                 Mac-2, mouse 
                 rat/mouse 
                 IgG2a; kappa 
                 M3/38.1.2.8 HL2 
                 TIB-166 
               
               
                 Mac-3 (mouse macrophage antigen, 110-kDa 
                 rat/mouse 
                 IgG1; kappa 
                 M3/84.6.34 
                 TIB-168 
               
               
                 glycoprotein) 
               
               
                 Macrophage, activated, mouse 
                 rat/mouse 
                 IgG2a 
                 158.2 
                 HB-8466 †   
               
               
                 Macrophage, human 
                 mouse 
                 IgG1; kappa 
                 14E5 
                 HB-174 
               
               
                 Macrophage, mouse 
                 rat/mouse 
                 IgG2b 
                 F4/80 
                 HB-198 
               
               
                 Macrophage, mouse 
                 rat/mouse 
                 IgG2b 
                 M1/70.15.11.5.HL 
                 TIB-128 
               
               
                 Macrophage, mouse 
                 rat/mouse 
                 IgG2a; kappa 
                 M3/38.1.2.8 HL2 
                 TIB-166 
               
               
                 Macrophage, pig 
                 mouse 
                 IgG1; kappa 
                 74-22-15 
                 HB-142 
               
               
                 Macrophage, pig 
                 mouse 
                 IgG2b; kappa 
                 74-22-15A 
                 HB-142.1 
               
               
                 Macrophage, pig 
                 mouse 
                 IgM; kappa 
                 76-5-28 
                 HB-153 
               
               
                 Macrophage, pig 
                 mouse 
                 IgM; kappa 
                 76-6-7 
                 HB-141 
               
               
                 MAdCAM-1, mouse 
                 rat/mouse 
                 IgG2a 
                 MECA-367 
                 HB-9478 †   
               
               
                 MAdCAM-1, mouse 
                 rat/mouse 
                 IgG2a 
                 MECA-89 
                 HB-292 
               
               
                 Malignant cultured cells, human 
                 mouse 
                 IgM 
                 B5 NIH 
                 HB-10569 †   
               
               
                 Mammalian H-Y antigen 
                 mouse 
                 IgM; kappa 
                 HY3-11.27 
                 HB-8116 
               
               
                 Mammalian sperm acrosomal vesicle 
                 mouse 
                 IgG1 
                 HS-21 (subclone 1H3) 
                 HB-255 
               
               
                 Mammalian splicing factor (SC35) 
                 mouse 
                 IgG1 
                 anti-SC35 
                 CRL-2031 
               
               
                 Mammary carcinoma cell line, human 
                 mouse 
                   
                 UCD/AB 6.01 
                 HB-8693 †   
               
               
                 Mammary carcinoma cell line, human 
                 mouse 
                   
                 UCD/AB 6.11 
                 HB-8458 †   
               
               
                 Mammary tumor cell cytoplasmic antigen, human 
                 mouse 
                 IgG1 
                 3B18 
                 HB-8654 †   
               
               
                 Mammary tumor cells, human 
                 mouse 
                 IgM 
                 B25.2 
                 HB-8107 †   
               
               
                 Mammary tumor cells, human 
                 mouse 
                 IgG1 
                 B38.1 
                 HB-8110 †   
               
               
                 Mammary tumor cells, human 
                 mouse 
                 IgG1 
                 B6.2 
                 HB-8106 †   
               
               
                 Mammary tumor cells, human 
                 mouse 
                 IgG1 
                 B72.3 
                 HB-8108 †   
               
               
                 Medullary thymic epithelium, mouse 
                 rat/mouse 
                 IgM 
                 MD2 
                 HB-229 
               
               
                 Melanoma associated antigens, human 
                 mouse 
                 IgG 
                 WI-MN-1 
                 HB-8672 †   
               
               
                 Melanoma cell line, human (M-1 antigen system) 
                 mouse 
                 IgG1 
                 LI 27 
                 HB-8437 †   
               
               
                 Melanoma cell line, human (M-11 antigen system) 
                 mouse 
                 IgG2a 
                 AL 1-27 
                 HB-8441 †   
               
               
                 Melanoma cell line, human (M-12 antigen system) 
                 mouse 
                 IgG1 
                 LI 66 
                 HB-8442 †   
               
               
                 Melanoma cell line, human (M-13 antigen system) 
                 mouse 
                 IgG1 
                 E 20 
                 HB-8443 †   
               
               
                 Melanoma cell line, human (M-16 antigen system) 
                 mouse 
                 IgM 
                 K 114 
                 HB-8444 †   
               
               
                 Melanoma cell line, human (M-18 antigen system) 
                 mouse 
                 IgG3 
                 R 24   
                 HB-8445 †   
               
               
                 Melanoma cell line, human (M-19 antigen system) 
                 mouse 
                 IgG1 
                 L235 
                 HB-8446 †   
               
               
                 Melanoma cell line, human (M-20 antigen system) 
                 mouse 
                 IgG2a 
                 L101 
                 HB-8447 †   
               
               
                 Melanoma cell line, human (M-23 antigen system) 
                 mouse 
                 IgG1; kappa 
                 L230 
                 HB-8448 †   
               
               
                 Melanoma cell line, human (M-26 antigen system) 
                 mouse 
                 IgG1 
                 A123 
                 HB-8451 †   
               
               
                 Melanoma cell line, human (M-27 antigen system) 
                 mouse 
                 IgM 
                 A124 
                 HB-8452 †   
               
               
                 Melanoma cell line, human (M-28 antigen system) 
                 mouse 
                 IgG2a 
                 B5 
                 HB-8453 †   
               
               
                 Melanoma tumor-specific antigen, human 
                 mouse 
                 IgG2a 
                 XMMME-001 
                 HB-8759 †   
               
               
                 Melanoma tumor-specific antigen, human 
                 mouse 
                 IgG2a 
                 XMMME-002 
                 HB-8760 †   
               
               
                 Mesothelial and ciliated cell protein, 130 kDa, 
                 mouse 
                 IgM; kappa 
                 anti-130-kDa 
                 CRL-2401 
               
               
                 human and rat 
                   
                   
                 Mesothelial-Ciliated Cells 
               
               
                 Microglobulin, beta-2, human 
                 mouse 
                 IgG2b 
                 BBM.1 
                 HB-28 
               
               
                 Microglobulin, beta-2, human 
                 mouse 
                 IgG1; kappa 
                 L368 
                 HB-149 
               
               
                 Microglobulin, beta-2, rat 
                 mouse 
                 IgG1 
                 4C9 
                 CRL-2437 
               
               
                 Monocyte, Fc receptor (high affinity, FcRI), human 
                 mouse 
                 IgG1 
                 32.2 
                 HB-9469 †   
               
               
                 Monocyte, human 
                 mouse 
                 IgG2b 
                 3C10 
                 TIB-228 
               
               
                 Monocyte, human 
                 mouse 
                 IgG2a; kappa 
                 4F2C13 
                 HB-22 
               
               
                 Monocyte, human 
                 mouse 
                 IgG1 
                 63D3 
                 HB-44 
               
               
                 Monocyte, human 
                 mouse 
                 IgM; kappa 
                 MMA 
                 HB-78 
               
               
                 Monocyte-derived neutrophil chemotactic factor, 
                 mouse 
                 IgG1 
                 EL-NC-1S 
                 HB-9647 †   
               
               
                 human 
               
               
                 Mononuclear cells, human 
                 mouse 
                 IgG2b 
                 OKM 1 
                 CRL-8026 
               
               
                 MOPC167 idiotype (V kappa 24) 
                 rat/mouse 
                 IgG1 and IgG2a 
                 28-6-20 
                 CRL-2489 
               
               
                 Mu heavy chain, human 
                 mouse 
                 IgG1; kappa 
                 DA4-4 
                 HB-57 
               
               
                 Mu heavy chain, mouse 
                 rat/mouse 
                 IgG2b 
                 331.12 
                 TIB-129 
               
               
                 Multidrug resistance protein (MRP) 
                 mouse 
                 IgG1 
                 QCRL-1 
                 HB-11765 †   
               
               
                 Multidrug resistance protein (MRP) 
                 mouse 
                 IgG2a 
                 QCRL-3 
                 HB-11766 †   
               
               
                 Muscle, heart 
                 mouse 
                 IgG2a; kappa 
                 356-1 
                 HB-181 
               
               
                 myb (c-myb), chicken 
                 mouse 
                 IgG2b 
                 MYB 2-3.76 
                 CRL-1728 
               
               
                 myb (c-myb), chicken 
                 mouse 
                 IgG2b 
                 MYB 2-37.63 
                 CRL-1726 
               
               
                 myb (c-myb), chicken 
                 mouse 
                 IgG1 
                 MYB 2-7.77 
                 CRL-1724 
               
               
                 myb (v-myb) 
                 mouse 
                 IgG2b 
                 MYB 2-3.76 
                 CRL-1728 
               
               
                 myb (v-myb) 
                 mouse 
                 IgG2b 
                 MYB 2-37.63 
                 CRL-1726 
               
               
                 myb (v-myb) 
                 mouse 
                 IgG1 
                 MYB 2-7.77 
                 CRL-1724 
               
               
                 myc (c-myc) protein, human 
                 mouse 
                 IgG1 
                 MYC 1-9E10.2 
                 CRL-1729 
               
               
                 myc (c-myc) protein, human 
                 mouse 
                 IgG1 
                 MYC CT 14-G4.3 
                 CRL-1727 
               
               
                 myc (c-myc) protein, human 
                 mouse 
                 IgG1 
                 MYC CT 9-B7.3 
                 CRL-1725 
               
               
                 Myeloid cell antigen, human 
                 mouse 
                 IgG1; kappa 
                 Anti-My-10 clone 
                 HB-8483 †   
               
               
                   
                   
                   
                 28/8/8/14/4 
               
               
                 Myeloid leukemia (CD33), human 
                 mouse 
                 IgG2a 
                 M195 
                 HB-10306 †   
               
               
                 Myocardium 
                 mouse 
                 IgG2a; kappa 
                 356-1 
                 HB-181 
               
               
                 Myosin heavy chain, adult, human 
                 mouse 
                 IgG2a 
                 A4.1025 
                 CRL-2044 
               
               
                 Myosin heavy chain, adult, slow, human and rodent 
                 mouse 
                 IgM 
                 A4.840 
                 CRL-2043 
               
               
                 Myosin heavy chain, cardiac alpha, rat 
                 mouse 
                 IgG2b 
                 BA-G5 
                 HB-276 
               
               
                 Myosin heavy chain, embryonic, human and rodent 
                 mouse 
                 IgG1 
                 F1.652 
                 CRL-2039 
               
               
                 Myosin heavy chain, embryonic, rat 
                 mouse 
                 IgG1 
                 BF-45 
                 HB-278 
               
               
                 Myosin heavy chain, embryonic, rat 
                 mouse 
                 IgG1 
                 BF-B6 
                 HB-279 
               
               
                 Myosin heavy chain, fast Ila, human and rodent 
                 mouse 
                 IgG1 
                 N2.261 
                 CRL-2047 
               
               
                 Myosin heavy chain, fast Ila, rodent 
                 mouse 
                 IgG1 
                 A4.74 
                 CRL-2041 
               
               
                 Myosin heavy chain, fast Ila, rodent 
                 mouse 
                 IgM 
                 N1.551 
                 CRL-2040 
               
               
                 Myosin heavy chain, fast, human 
                 mouse 
                 IgG1 
                 A4.74 
                 CRL-2041 
               
               
                 Myosin heavy chain, neonatal and adult fast, human 
                 mouse 
                 IgM 
                 N3.36 
                 CRL-2042 
               
               
                 Myosin heavy chain, slow, human and rodent 
                 mouse 
                 IgG1 
                 A4.951 
                 CRL-2046 
               
               
                 Myosin heavy chain, slow, human and rodent 
                 mouse 
                 IgG1 
                 N2.261 
                 CRL-2047 
               
               
                 Myosin heavy chain, type 1, rat 
                 mouse 
                 IgG1 
                 BA-D5 
                 HB-287 
               
               
                 Myosin heavy chain, type 2A, rat 
                 mouse 
                 IgG1 
                 SC-71 
                 HB-277 
               
               
                 Myosin heavy chain, type 2B, rat 
                 mouse 
                 IgM 
                 BF-F3 
                 HB-283 
               
               
                 NAP-1 (neutrophil attractant/activation protein 1) 
                 mouse 
                 IgG1 
                 EL-NC-1S 
                 HB-9647 †   
               
               
                 Nerve growth factor (NGF) receptor, primate 
                 mouse 
                 IgG1 
                 200-3-G6-4 (20.4) 
                 HB-8737 †   
               
               
                 Neuroblastoma, human 
                 mouse 
                 IgM 
                 PI 153/3 
                 TIB-198 
               
               
                 Neutrophil attractant/activation protein 1, human 
                 mouse 
                 IgG1 
                 EL-NC-1S 
                 HB-9647 †   
               
               
                 Neutrophils, mouse 
                 rat/mouse 
                 IgM 
                 J11d.2 
                 TIB-183 
               
               
                 nG4m(b) isoallotope, human 
                 mouse 
                 IgG1 
                 HP6016 
                 CRL-1787 
               
               
                 Nicotinic acetylcholine receptor,  Torpedo californica   
                 mouse 
                 IgG1 
                 88B 
                 CRL-1967 
               
               
                 NK cell antigen, mouse (LGL-1) 
                 rat/mouse 
                 IgG2a 
                 4D11 
                 HB-240 
               
               
                 NK cell target ligand on NC-37 cells 
                 mouse 
                 IgM 
                 18C2.8.3 
                 HB-9571 †   
               
               
                 NK cell target ligand on NC-37 cells 
                 mouse 
                 IgM 
                 7C6.5.4 
                 HB-9574 †   
               
               
                 NK cells, human 
                 mouse 
                 IgM; kappa 
                 HNK-1 
                 TIB-200 
               
               
                 NK cells, mouse 
                 mouse 
                 IgG2a 
                 PK136 
                 HB-191 
               
               
                 Non-small cell lung carcinoma (NSCLC), human 
                 mouse 
                 IgG1 
                 L18 
                 HB-8628 †   
               
               
                 Non-small cell lung carcinoma (NSCLC), human 
                 mouse 
                 IgM 
                 L5 
                 HB-8627 †   
               
               
                 O-antigen,  Escherichia coli  O157 
                 mouse 
                 IgM 
                 MARC 29F8 
                 CRL-2508 
               
               
                 O-antigen,  Escherichia coli  O157 
                 mouse 
                 IgM 
                 MARC S5 
                 CRL-2507 
               
               
                 OKT-10 like molecule, human 
                 mouse 
                 IgG1 
                 THB-7 
                 HB-136 
               
               
                 o-Phosphotyrosine 
                 mouse 
                 IgG1; kappa 
                 2G8.D6 
                 HB-8190 †   
               
               
                 Ornithine decarboxylase (ODC), mouse 
                 mouse 
                 IgM 
                 B11 
                 HB-8372 †   
               
               
                 Ovarian carcinoma cell line (2774), human 
                 mouse 
                 IgG1 
                 ME195 
                 HB-8431 †   
               
               
                 Ovarian carcinoma cell line (2774), human 
                 mouse 
                 IgG2a 
                 MF 116 
                 HB-8411 †   
               
               
                 Ovarian carcinoma cell lines, human 
                 mouse 
                 IgM 
                 MH55 
                 HB-8412 †   
               
               
                 Ovarian carcinoma, human 
                 mouse 
                 IgG2a; IgG2b 
                 OVB-3 
                 HB-9147 †   
               
               
                 Oxysterol binding protein 
                 mouse 
                 IgG2a 
                 IgG-B16 
                 CRL-1899 
               
               
                 Oxysterol binding protein (OSBP), rabbit 
                 mouse 
                 IgG1 
                 IgG-11H9 
                 CRL-2213 
               
               
                 Oxytocin-neurophysin (NP-OT), rat 
                 mouse 
                 IgG2b; kappa 
                 PS 38 
                 CRL-1950 
               
               
                 Oxytocin-neurophysin (NP-OT), rat 
                 mouse 
                 IgG2b; kappa 
                 PS 60 
                 CRL-1800 
               
               
                 Oxytocin-neurophysin (NP-OT), rat 
                 mouse 
                 IgG2a; kappa 
                 PS 67 
                 CRL-1797 
               
               
                 p12 gag protein of murine leukemia viruses (MuLV) 
                 mouse 
                 IgG2b 
                 548 
                 CRL-1890 
               
               
                 p15 gag protein of murine leukemia viruses (MuLV) 
                 mouse 
                 IgG2b 
                 34 
                 CRL-1889 
               
               
                 p15E env protein of murine leukemia viruses (MuLV) 
                 mouse 
                 IgG3 
                 372 
                 CRL-1893 
               
               
                 p30 gag protein of murine leukemia virus (MuLV) 
                 rat/mouse 
                 IgG1 
                 R187 
                 CRL-1912 
               
               
                 Parainfluenzavirus type 3, fusion glycoprotein (F), 
                 mouse 
                 IgG1 
                 9-4-3 
                 HB-8935 †   
               
               
                 human 
               
               
                 Parainfluenzavirus type 3, hemagglutinin (HN), 
                 mouse 
                 IgG2a 
                 13-5-9-6-2 
                 HB-8934 †   
               
               
                 human 
               
               
                 Paramyosin,  Schistosoma mansoni   
                 mouse 
                 IgG2a 
                 MBL-Sm-1A6 
                 HB-194 
               
               
                 Paramyosin,  Schistosoma mansoni   
                 mouse 
                 IgG2a 
                 MBL-Sm-4B1 
                 HB-193 
               
               
                 Pasturella multocida type D dermonecrotic toxin 
                 mouse 
                 IgG1 
                 1B2A3 
                 CRL-1965 
               
               
                 PDGF B, v-sis form 
                 mouse 
                 IgG2a 
                 116 
                 HB-9367 †   
               
               
                 PDGF B, v-sis form 
                 mouse 
                 IgG2b 
                 232 
                 HB-9372 †   
               
               
                 PDGF B, v-sis form 
                 mouse 
                 IgG1 
                 52 
                 HB-9361 †   
               
               
                 Peptidoglycan, bacterial 
                 mouse 
                 IgG3; kappa 
                 15B2 
                 HB-8510 †   
               
               
                 Peptidoglycan, bacterial 
                 mouse 
                 IgG1; kappa 
                 3C11 
                 HB-8511 †   
               
               
                 Peptidoglycan, bacterial 
                 mouse 
                 IgM; kappa 
                 3F6 
                 HB-8512 †   
               
               
                 Peptidoglycan, bacterial 
                 mouse 
                 IgM; lambda 
                 3G3 
                 HB-8516 †   
               
               
                 Periostin 
                 mouse 
                 IgG1; kappa 
                 5H8 
                 CRL-2646 
               
               
                 PETA-3 (CD151) 
                 mouse 
                 IgG1 
                 41-2 
                 CRL-2695 
               
               
                 PETA-3 (CD151) 
                 mouse 
                 IgG1 
                 50-6 
                 CRL-2696 
               
               
                 Peyer&#39;s patch endothelial cells, human 
                 mouse 
                 IgG2a 
                 Hermes-3 
                 HB-9480 †   
               
               
                 Peyer&#39;s patch endothelial cells, mouse 
                 rat/mouse 
                 IgG2a 
                 MECA-367 
                 HB-9478 †   
               
               
                 P-glycoprotein, human 
                 mouse 
                 IgG2b 
                 443-17F9-1C6 
                 CRL-2694 
               
               
                 Pgp-1 glycoprotein, mouse 
                 rat/mouse 
                 IgG2b 
                 IM7.8.1 
                 TIB-235 
               
               
                 Pgp-1, mouse 
                 rat/mouse 
                 IgG1 
                 KM114 
                 TIB-242 
               
               
                 Pgp-1, mouse 
                 rat/mouse 
                 IgG1 
                 KM201 
                 TIB-240 
               
               
                 Pgp-1, mouse 
                 rat/mouse 
                 IgG2a 
                 KM703 
                 CRL-1896 
               
               
                 Pgp-1, mouse 
                 rat/mouse 
                 IgG2a 
                 KM81 
                 TIB-241 
               
               
                 Phenylarsonate 
                 mouse 
                 IgE 
                 SE-1.3 
                 HB-137 
               
               
                 Phosphatidylinositol 4-kinase, type II, bovine 
                 mouse 
                 IgG1; kappa 
                 4C5G 
                 CRL-2538 
               
               
                 Phosphotyrosine 
                 mouse 
                 IgG1; kappa 
                 2G8.D6 
                 HB-8190 †   
               
               
                 Phosphotyrosine 
                 mouse 
                 IgG3; kappa 
                 FB2 
                 CRL-1891 
               
               
                 Phosphotyrosine 
                 mouse 
                 IgG1 
                 P-tyr-1 
                 CRL-1955 
               
               
                 Plasmodium falciparum merozoite antigen 
                 mouse 
                 IgG2b 
                 MAb 5.2 
                 HB-9148 
               
               
                 Platelet glycoprotein GPIIIa, human 
                 mouse 
                 IgG1; kappa 
                 LK-4 
                 CRL-2345 
               
               
                 Platelet-derived growth factor B chain (PDGF B, 
                 mouse 
                 IgG2a 
                 116 
                 HB-9367 †   
               
               
                 v-sis form) 
               
               
                 Platelet-derived growth factor B chain (PDGF B, 
                 mouse 
                 IgG2b 
                 232 
                 HB-9372 †   
               
               
                 v-sis form) 
               
               
                 Platelet-derived growth factor B chain (PDGF B, 
                 mouse 
                 IgG1 
                 52 
                 HB-9361 †   
               
               
                 v-sis form) 
               
               
                 Platelets, human 
                 mouse 
                 IgG1 
                 7E3 
                 HB-8832 †   
               
               
                 p-nitroanaline amide derivatives 
                 mouse 
                 IgG1 
                 P3 6D4 (SCRF 43.1) 
                 HB-9168 †   
               
               
                 p-nitroanaline amide derivatives 
                 mouse 
                 IgG1 
                 P3 8D2 (SCRF 43.1) 
                 HB-9169 †   
               
               
                 p-nitroanaline amide derivatives 
                 mouse 
                 IgG1 
                 QPN1 12C9 (SCRF 43.2) 
                 HB-9500 †   
               
               
                 p-nitroanaline amide derivatives 
                 mouse 
                 IgG1 
                 QPN1 22F5 (SCRF 43.2) 
                 HB-9509 †   
               
               
                 Polypeptide, synthetic 
                 mouse 
                 IgM 
                 7C8 
                 HB-8465 †   
               
               
                 Polypeptide, synthetic, Asp-Tyr-Lys-Asp-Asp-Asp- 
                 mouse 
                 IgG2b 
                 4E11 
                 HB-9259 †   
               
               
                 Asp-Lys 
               
               
                 Polypyrimidine tract binding protein (PTB) 
                 mouse 
                 IgG2b; kappa 
                 mAb BB7 
                 CRL-2501 
               
               
                 Pig parvovirus (PPV) 
                 mouse 
                 IgG1 
                 3C9-D11-H11 
                 CRL-1745 
               
               
                 Primate tissue, normal 
                 mouse 
                 IgM 
                 B5 NIH 
                 HB-10569 †   
               
               
                 Prostate antigen (PA), human 
                 mouse 
                 IgM; kappa 
                 F5-A-1/22.8.13 
                 HB-8051 †   
               
               
                 Prostate antigen (PA), human 
                 mouse 
                 IgG1 
                 RLSD06 
                 HB-8527 †   
               
               
                 Prostate antigen (PA), human 
                 mouse 
                 IgG1 
                 RLSD09 
                 HB-8525 †   
               
               
                 Prostate cancer antigen, human 
                 mouse 
                 IgG1 
                 7E11C5 
                 HB-10494 †   
               
               
                 Prostate cancer, human 
                 mouse 
                 IgG3 
                 P25.48 
                 HB-9119 †   
               
               
                 Prostate epithelial cells 
                 mouse/mouse 
                 IgG1 
                 Prost 410 
                 HB-11426 †   
               
               
                 Prothrombin, abnormal, human 
                 mouse 
                 IgG1 
                 JO1-1 
                 HB-8638 †   
               
               
                 P-selectin, human 
                 mouse 
                 IgG1 
                 WAPS 12.2 
                 HB-299 
               
               
                   Pseudomonas aeruginosa  (flagella type b) 
                 human 
                   
                 20H11 
                 CRL-9300 †   
               
               
                   Pseudomonas aeruginosa  lipopolysaccharide (LPS) 
                 human 
                 IgM 
                 C5B7 
                 CRL-8753 †   
               
               
                 Fisher immunotype 1 (IATS type 6) 
               
               
                   Pseudomonas aeruginosa  lipopolysaccharide (LPS) 
                 human 
                 IgM 
                 9D10 
                 CRL-8752 †   
               
               
                 Fisher immunotype 4 (IATS type 1) 
               
               
                   Pseudomonas aeruginosa  lipopolysaccharide (LPS) 
                 human 
                 IgM 
                 8E7 
                 CRL-8795 †   
               
               
                 Fisher immunotype 7 
               
               
                 Pseudorabies virus (PRV) 
                 mouse 
                 IgG2b 
                 3G9F3 
                 CRL-1843 
               
               
                 Pseudorabies virus (PRV) 
                 mouse 
                 IgG2b 
                 6D8MB4 
                 CRL-1842 
               
               
                 RAN-2 (rat neural antigen-2) 
                 mouse 
                 IgG2a 
                 Ran-2 
                 TIB-119 
               
               
                 ras (c-ras) protein, p21 
                 rat 
                 IgG2a 
                 Y13-238 
                 CRL-1741 
               
               
                 ras (c-ras) protein, p21 
                 rat 
                 IgG1 
                 Y13-259 
                 CRL-1742 
               
               
                 ras (v-ras K) oncogene peptide, synthetic 
                 mouse 
                 IgG1 and IgG2b 
                 147-67C6 
                 CRL-2654 
               
               
                 ras (v-ras) protein, p21 
                 rat 
                 IgG2a 
                 Y13-238 
                 CRL-1741 
               
               
                 ras (v-ras) protein, p21 
                 rat 
                 IgG1 
                 Y13-259 
                 CRL-1742 
               
               
                 ras oncogene peptide, synthetic 
                 mouse 
                 IgG1; kappa 
                 146-03E04 
                 CRL-2650 
               
               
                 ras, H/N, peptide, synthetic 
                 mouse 
                 IgG1; kappa 
                 142-24E5 
                 CRL-2649 
               
               
                 ras, Ha, p21 
                 mouse 
                 IgG1 
                 MX 
                 HB-9158 †   
               
               
                 Rat neural antigen-2 (RAN-2) 
                 mouse 
                 IgG2a 
                 RAN-2 
                 TIB-119 
               
               
                 Receptor, 1,25-dihydroxy vitamin D3, pig 
                 mouse 
                 IgG1 
                 XVI E6E6G10 
                 HB-9496 †   
               
               
                 Receptor, acetylcholine, neuronal, chicken 
                 rat/mouse 
                 IgG2a 
                 mAb 270 
                 HB-189 
               
               
                 Receptor, acetylcholine, neuronal, rat 
                 rat/mouse 
                 IgG2a 
                 mAb 270 
                 HB-189 
               
               
                 Receptor, CD28, mouse 
                 hamster/mouse 
                 IgG 
                 PV1 
                 HB-12352 †   
               
               
                 Receptor, complement, type 3 (CR3), mouse 
                 rat/mouse 
                 IgG2b 
                 5C6 Clone 1 
                 CRL-1969 
               
               
                 Receptor, Coxsackievirus-adenovirus, human 
                 mouse 
                 IgG1 
                 RmcB 
                 CRL-2379 
               
               
                 Receptor, epidermal growth factor (EGF) 
                 mouse 
                 IgG1 
                 225 
                 HB-8508 †   
               
               
                 Receptor, epidermal growth factor (EGF) 
                 mouse 
                 IgG1 
                 455 
                 HB-8507 †   
               
               
                 Receptor, epidermal growth factor (EGF) 
                 mouse 
                 IgG2a 
                 528 
                 HB-8509 †   
               
               
                 Receptor, epidermal growth factor (EGF) 
                 mouse 
                 IgG 
                 579 
                 HB-8506 †   
               
               
                 Receptor, epidermal growth factor (EGF), human 
                 mouse 
                 IgG2a; kappa 
                 Mab 108 
                 HB-9764 †   
               
               
                 Receptor, epidermal growth factor (EGF), human 
                 mouse 
                 IgM 
                 Mab 96 
                 HB-9763 †   
               
               
                 Receptor, Epstein-Barr virus (EBV) 
                 mouse 
                 IgG2a; kappa 
                 THB-5 
                 HB-135 
               
               
                 Receptor, Fc alpha, human 
                 mouse 
                 IgM 
                 My 43.51 
                 HB-12128 †   
               
               
                 Receptor, follicle stimulating hormone (FSH), human 
                 mouse 
                 IgG1 
                 FSHR-18 
                 CRL-2688 
               
               
                 Receptor, insulin, human 
                 mouse 
                 IgG1; kappa 
                 αIR-1 
                 HB-175 
               
               
                 Receptor, insulin, placental, human 
                 mouse 
                 IgG1 
                 DII 33.1 
                 CRL-1827 
               
               
                 Receptor, interferon gamma, mouse 
                 rat/mouse 
                 IgG2a; kappa 
                 GR-20 
                 CRL-2024 
               
               
                 Receptor, interferon gamma, mouse 
                 rat/mouse 
                 IgA; kappa 
                 GR-96 
                 CRL-2013 
               
               
                 Receptor, interleukin 12 (IL-12), beta 1 subunit, 
                 rat/mouse 
                 IgG2a 
                 HIL12R1.2B10 
                 CRL-2359 
               
               
                 human 
               
               
                 Receptor, interleukin 2 (IL-2), human 
                 mouse 
                 IgG1 
                 2A3A1H 
                 HB-8555 †   
               
               
                 Receptor, interleukin 2 (IL-2), human 
                 mouse 
                 IgG2a 
                 7G7B6 
                 HB-8784 †   
               
               
                 Receptor, interleukin 2 (IL-2), mouse 
                 rat/mouse 
                 IgM; kappa 
                 7D4 
                 CRL-1698 
               
               
                 Receptor, interleukin 2 (IL-2), mouse 
                 rat/mouse 
                 IgG1 
                 PC 61 5.3 
                 TIB-222 
               
               
                 Receptor, interleukin 8, type B, human 
                 mouse 
                 IgG2a 
                 10H2.12.1 
                 HB-11494 †   
               
               
                 Receptor, interleukin 8, type B, human 
                 mouse 
                 IgG2a 
                 4D1.5.7 
                 HB-11495 †   
               
               
                 Receptor, luteinizing hormone/chorionic 
                 mouse 
                 IgG2a 
                 FSHR-323 
                 CRL-2689 
               
               
                 gonadotropic (LH/hCG), human 
               
               
                 Receptor, luteinizing hormone/chorionic 
                 mouse 
                 IgG1 
                 LHR-1055 
                 CRL-2687 
               
               
                 gonadotropic (LH/hCG), human 
               
               
                 Receptor, luteinizing hormone/chorionic 
                 mouse 
                 IgG1 
                 LHR-29 
                 CRL-2685 
               
               
                 gonadotropic (LH/hCG), human 
               
               
                 Receptor, luteinizing hormone/chorionic 
                 mouse 
                 IgG1 
                 LHR-74 
                 CRL-2686 
               
               
                 gonadotropic (LH/hCG), human 
               
               
                 Receptor, nerve growth factor (NGF), primate 
                 mouse 
                 IgG1 
                 200-3-G6-4 (20.4) 
                 HB-8737 †   
               
               
                 Receptor, stem cell factor (SCF), human 
                 mouse 
                 IgG2a 
                 BA7.3C.9 
                 HB-10716 †   
               
               
                 Receptor, transferrin, human 
                 mouse 
                 IgG2a 
                 L5.1 
                 HB-84 
               
               
                 Receptor, transferrin, human 
                 mouse 
                 IgG1 
                 OKT 9 
                 CRL-8021 
               
               
                 Receptor, transferrin, mouse 
                 rat/mouse 
                 IgM 
                 R17 208.2 
                 TIB-220 
               
               
                 Receptor, transferrin, mouse 
                 rat/mouse 
                 IgG2a 
                 R17 217.1.3 
                 TIB-219 
               
               
                 Receptor, vascular endothelial growth factor (VEGF), 
                 rat/mouse 
                 IgG1; kappa 
                 DC101 
                 HB-11534 †   
               
               
                 mouse 
               
               
                 Receptor, very low density lipoprotein (VLDL) 
                 mouse 
                 IgG1 
                 IgG-6A6 
                 CRL-2197 
               
               
                 Receptor, vitonectin, human 
                 mouse 
                 IgG1 
                 B6H12.2 
                 HB-9771 †   
               
               
                 Red blood cells, sheep 
                 mouse 
                 IgM; lambda 
                 N-S.2.1 
                 TIB-108 
               
               
                 Red blood cells, sheep 
                 mouse 
                 IgM; kappa 
                 N-S.4.1 
                 TIB-110 
               
               
                 Red blood cells, sheep 
                 mouse 
                 IgG3; kappa 
                 N-S.7 
                 TIB-114 
               
               
                 Red blood cells, sheep 
                 mouse 
                 IgG2b 
                 N-S.8.1 
                 TIB-109 
               
               
                 Red blood cells, sheep 
                 mouse 
                 IgG2a; kappa 
                 S-S.1 
                 TIB-111 
               
               
                 Red blood cells, sheep 
                 mouse 
                 IgM; kappa 
                 S-S.3 
                 TIB-112 
               
               
                 Renal carcinoma cell lines, human 
                 mouse 
                 IgG1 
                 ME195 
                 HB-8431 †   
               
               
                 Renal carcinoma cell lines, human 
                 mouse 
                 IgG2a 
                 MF 116 
                 HB-8411 †   
               
               
                 Renal cell carcinoma, human 
                 mouse 
                 IgG1 
                 DAL K20 
                 CRL-2288 
               
               
                 Renal cell carcinoma, human 
                 mouse 
                 IgG1 
                 DAL K29 
                 CRL-2291 
               
               
                 Renal cell carcinoma, human 
                 mouse 
                 IgG1 
                 DAL K45 
                 CRL-2292 
               
               
                 Renin, hog 
                 mouse 
                 IgG1 
                 F32 VIII C4 
                 CRL-1653 
               
               
                 Reovirus type 3, sigma 1 hemagglutinin 
                 mouse 
                 IgG2a 
                 9BG5 
                 HB-167 
               
               
                 REP-1, human 
                 mouse 
                 IgG1 
                 IgG-2F1 
                 CRL-2419 
               
               
                 Retinal Muller cell, rat 
                 mouse 
                 IgG2a 
                 RAN-2 
                 TIB-119 
               
               
                 Retinoblastoma, human 
                 mouse 
                 IgM 
                 PI 153/3 
                 TIB-198 
               
               
                 Ricin, A chain (A1 and A2) 
                 mouse 
                 IgG1 
                 TFTA1 
                 CRL-1771 
               
               
                 Ricin, B chain 
                 mouse 
                 IgG1 
                 TFTB1 
                 CRL-1759 
               
               
                 RNA-DNA hybrids 
                 mouse 
                 IgG 
                 S9.6 
                 HB-8730 †   
               
               
                 Saccharide antigen, Gal beta1-3GalNAc (T antigen) 
                 mouse 
                 IgG3; kappa 
                 JAA-F11 
                 CRL-2381 
               
               
                 Saxitoxin binding component of electroplex 
                 mouse 
                 IgG1 
                 VD-10 
                 HB-68 
               
               
                 membrane 
               
               
                 Sca-1, mouse 
                 rat/mouse 
                 IgG2a 
                 E13 161-7 
                 HB-215 
               
               
                 SCAP 
                 mouse 
                 IgG2b 
                 IgG-9D5 
                 CRL-2347 
               
               
                   Schistosoma mansoni  surface (cercariae) 
                 mouse 
                 IgA 
                 129A3/1 
                 HB-8087 †   
               
               
                 glycoprotein 
               
               
                   Schistosoma mansoni  surface (cercariae) 
                 mouse 
                 IgG1 
                 130C3/2B/8 
                 HB-8088 †   
               
               
                 glycoprotein 
               
               
                   Schistosoma mansoni  surface (cercariae) 
                 mouse 
                 IgG1 
                 132C4A/4 
                 HB-8086 †   
               
               
                 glycoprotein 
               
               
                 L-Selectin, human 
                 mouse 
                 IgG1 
                 DREG200 
                 HB-302 
               
               
                 L-Selectin, human 
                 mouse 
                 IgG1 
                 DREG56 
                 HB-300 
               
               
                 L-Selectin, mouse 
                 rat/mouse 
                 IgG2a 
                 MEL-14 
                 HB-132 
               
               
                 L-Selectin, sheep and bovine 
                 mouse 
                 IgG1 
                 DU1-29 
                 HB-263 
               
               
                 Sex lethal gene product (Sxl), female specific, 
                 mouse 
                 IgG1 
                 mSXL 104 
                 CRL-1953 
               
               
                 
                   Drosophila melanogaster 
                 
               
               
                 Sex lethal gene product (Sxl), female specific, 
                 mouse 
                 IgG1 
                 mSXL 114 
                 CRL-1954 
               
               
                 
                   Drosophila melanogaster 
                 
               
               
                 Sex lethal gene product (Sxl), female specific, 
                 mouse 
                 IgG1 
                 mSXL 18 
                 CRL-1952 
               
               
                 
                   Drosophila melanogaster 
                 
               
               
                 Sex lethal gene product (Sxl), female specific, 
                 mouse 
                 IgG1 
                 mSXL 5 
                 CRL-1951 
               
               
                 
                   Drosophila melanogaster 
                 
               
               
                 Shiga toxin 
                 mouse 
                 IgG1; kappa 
                 13C4 
                 CRL-1794 
               
               
                 Shiga-like toxin I (SLTI) 
                 mouse 
                 IgG1; kappa 
                 13C4 
                 CRL-1794 
               
               
                 Shiga-like toxin II (SLT-II) 
                 mouse 
                 IgG1; kappa 
                 11E10 
                 CRL-1907 
               
               
                 Shiga-like toxin II (SLT-II) 
                 mouse 
                 IgG2a; kappa 
                 11F11 
                 CRL-1908 
               
               
                 SLA a, c, d 
                 mouse 
                 IgG2a 
                 7-34-1 
                 CRL-1945 
               
               
                 SLA ABd (pig histocompatibility antigen) 
                 mouse 
                 IgG2b; kappa 
                 74-11-10 
                 HB-139 
               
               
                 SR proteins (pre-mRNA splicing factors) 
                 mouse 
                 IgG1 
                 anti-SR (1H4) 
                 CRL-2383 
               
               
                 SR proteins (pre-mRNA splicing factors) 
                 mouse 
                 IgM 
                 MAb104 
                 CRL-2067 
               
               
                 SR proteins, conserved epitope 
                 mouse 
                 IgG1 
                 16H3 
                 CRL-2385 
               
               
                 src (v-src) oncogene peptide, synthetic 
                 mouse 
                 IgG1 
                 201-45E9 
                 CRL-2670 
               
               
                 src (v-src) oncogene peptide, synthetic 
                 mouse 
                 IgG2a; IgG2b 
                 203-7D10 
                 CRL-2651 
               
               
                 src/yes oncogene peptide, synthetic 
                 mouse 
                 IgG1 
                 202-11A8 
                 CRL-2669 
               
               
                 SREBP cleavage activating protein 
                 mouse 
                 IgG2b 
                 IgG-9D5 
                 CRL-2347 
               
               
                 SREBP-2, hamster 
                 mouse 
                 IgG2b 
                 IgG-7D4 
                 CRL-2198 
               
               
                 SRp20 proteins (pre-mRNA splicing factors) 
                 mouse 
                 IgG1 
                 anti-SRp20 (7B4) 
                 CRL-2384 
               
               
                 Stem cell antigen 1, mouse (Sca-1) 
                 rat/mouse 
                 IgG2a 
                 E13 161-7 
                 HB-215 
               
               
                 Stem cell factor (SCF) receptor, human 
                 mouse 
                 IgG2a 
                 BA7.3C.9 
                 HB-10716 †   
               
               
                 Stem cells, mesenchymal, human 
                 mouse 
                 IgG1 
                 SH2 
                 HB-10743 †   
               
               
                 Stem cells, mesenchymal, human 
                 mouse 
                 IgG2b 
                 SH3 
                 HB-10744 †   
               
               
                 Stem cells, mesenchymal, human 
                 mouse 
                 IgG1 
                 SH4 
                 HB-10745 †   
               
               
                 Sterol regulatory element binding protein (dSREBP), 
                 mouse 
                 IgG1; kappa 
                 IgG-3B2 
                 CRL-2693 
               
               
                 
                   Drosophila melanogaster 
                 
               
               
                 Sterol regulatory element binding protein (SREBP), 
                 mouse 
                 IgG1 
                 IgG-2A4 
                 CRL-2121 
               
               
                 human 
               
               
                 Sterol regulatory element binding protein 2 
                 mouse 
                 IgG2b 
                 IgG-7D4 
                 CRL-2198 
               
               
                 (SREBP-2), hamster 
               
               
                 Sterol regulatory element binding protein 2 
                 mouse 
                 IgG1 
                 IgG-1C6 
                 CRL-2224 
               
               
                 (SREBP-2), human 
               
               
                 Sterol regulatory element binding protein 2 
                 mouse 
                 IgG1; kappa 
                 IgG-1D2 
                 CRL-2545 
               
               
                 (SREBP-2), human 
               
               
                 
                   Streptococcus mutans 
                 
                 mouse 
                 IgG2a; kappa 
                 SWLA1 
                 HB-12559 †   
               
               
                 
                   Streptococcus mutans 
                 
                 mouse 
                 IgG2a; kappa 
                 SWLA2 
                 HB-12560 †   
               
               
                 
                   Streptococcus mutans 
                 
                 mouse 
                   
                 SWLA3 
                 HB-12558 †   
               
               
                 SV40 T antigen 
                 mouse 
                 IgG2a 
                 PAb 101 
                 TIB-117 
               
               
                 SV40 T antigen 
                 mouse 
                 IgG1 
                 PAb 100 
                 TIB-115 
               
               
                 SV40 T antigen, N terminal 
                 mouse 
                 IgG2a 
                 PAb 108 
                 TIB-230 
               
               
                 SV40 T antigen, N terminal 
                 mouse 
                 IgG2a 
                 PAb 108 
                 TIB-230 
               
               
                 Swine leucocyte antigen (SLA) 
                 mouse 
                 IgG2a 
                 7-34-1 
                 CRL-1945 
               
               
                 T antigen, N terminal, SV40 
                 mouse 
                 IgG2a 
                 PAb 108 
                 TIB-230 
               
               
                 T antigen, non-viral (NVT), mouse 
                 mouse 
                 IgG2b 
                 PAb 122 
                 TIB-116 
               
               
                 T antigen, SV40 
                 mouse 
                 IgG2a 
                 PAb 101 
                 TIB-117 
               
               
                 T antigen, SV40 
                 mouse 
                 IgG1 
                 PAb 100 
                 TIB-115 
               
               
                 T cell (activated), human 
                 mouse 
                 IgG1 
                 10D2F6 
                 HB-11103 †   
               
               
                 T cell (activated), human 
                 mouse 
                 IgG1 
                 OKT 9 
                 CRL-8021 
               
               
                 T cell antigen receptor (Jurkat cells), human 
                 mouse 
                 IgM; kappa 
                 C305 
                 CRL-2424 
               
               
                 T cell antigen receptor, gamma/delta negative, sheep 
                 mouse 
                 IgG1; kappa 
                 86D 
                 HB-286 
               
               
                 T cell antigen receptor, human, major framework 
                 mouse 
                 IgG1 
                 (BF1) 8A3.31 
                 HB-9283 †   
               
               
                 determinant 
               
               
                 T cell antigen receptor, human, major framework 
                 mouse 
                 IgG2a 
                 W4F.5B 
                 HB-9282 †   
               
               
                 determinant 
               
               
                 T cell antigen receptor, mouse 
                 hamster/mouse 
                 IgG 
                 H57-597 
                 HB-218 
               
               
                 T cell antigen receptor, mouse 
                 rat/mouse 
                 IgG2b 
                 TR 310 
                 HB-219 
               
               
                 T cell precursor, human 
                 mouse 
                 IgG1 
                 OKT 10 
                 CRL-8022 
               
               
                 T cell receptor, gamma/delta, mouse 
                 hamster/mouse 
                 IgG 
                 UC3-10A6 
                 CRL-1988 
               
               
                 T cell receptor, gamma/delta, mouse 
                 hamster/mouse 
                 IgG 
                 UC7-13D5 
                 CRL-1989 
               
               
                 T cells, cytotoxic, pig 
                 mouse 
                 IgG2a; kappa 
                 76-2-11 
                 HB-143 
               
               
                 T cells, cytotoxic/suppressor, human 
                 mouse 
                 IgG1 
                 OKT 5 
                 CRL-8013 
               
               
                 T cells, cytotoxic/suppressor, human 
                 mouse 
                 IgG1 
                 OKT 5 
                 CRL-8016 
               
               
                 T cells, cytotoxic/suppressor, human 
                 mouse 
                 IgG2a 
                 OKT 8 
                 CRL-8014 
               
               
                 T cells, gamma/delta positive, bovine 
                 mouse 
                 IgG1 
                 IL-A29 
                 CRL-1874 
               
               
                 T cells, helper/inducer, human 
                 mouse 
                 IgG2b 
                 OKT 4 
                 CRL-8002 
               
               
                 T cells, helper/inducer, mouse 
                 rat/mouse 
                 IgG2b 
                 GK1.5 
                 TIB-207 
               
               
                 T cells, human 
                 mouse 
                 IgM 
                 2T8-3E10 
                 HB-8213 †   
               
               
                 T cells, human 
                 mouse 
                 IgM 
                 3Pt12B8 
                 HB-8136 †   
               
               
                 T cells, human 
                 mouse 
                 IgG1; kappa 
                 5E9C11 
                 HB-21 
               
               
                 T cells, human 
                 mouse 
                 IgG1 
                 OKT 1 
                 CRL-8000 
               
               
                 T cells, human 
                 mouse 
                 IgG1 
                 OKT 11 
                 CRL-8027 
               
               
                 T cells, human 
                 mouse 
                 IgG2a 
                 OKT 3 
                 CRL-8001 
               
               
                 T cells, human 
                 mouse 
                 IgG1; kappa 
                 T3-3A1 
                 HB-2 
               
               
                 T cells, mouse 
                 mouse 
                 IgM; kappa 
                 20-10-5S 
                 HB-23 
               
               
                 T cells, pig 
                 mouse 
                 IgM; kappa 
                 76-5-28 
                 HB-153 
               
               
                 T cells, pig 
                 mouse 
                 IgM; kappa 
                 76-6-7 
                 HB-141 
               
               
                 T cells, rabbit 
                 mouse 
                 IgM 
                 9AE10 
                 CRL-1761 
               
               
                 T cells, rabbit 
                 mouse 
                 IgG1 
                 L11/135 
                 TIB-188 
               
               
                 T12 (120 kDa) antigen, human T cells 
                 mouse 
                 IgM 
                 3Pt12B8 
                 HB-8136 †   
               
               
                 T15 idiotype 
                 mouse 
                 IgG1; kappa 
                 AB1-2 
                 HB-33 
               
               
                 TAG-72 (tumor-associated glycoprotein), human 
                 mouse 
                 IgG1; kappa 
                 CC 49 
                 HB-9459 †   
               
               
                 T-B activating molecule (T-BAM), human 
                 mouse 
                 IgG2a 
                 5c8 
                 HB-10916 †   
               
               
                 Tetanus toxin 
                 human/mouse 
                 IgG1 
                 9F12 
                 HB-8177 †   
               
               
                 Tetanus toxoid 
                 human 
                 IgG; kappa 
                 SA13 
                 HB-8501 †   
               
               
                 TGF-beta-2, mouse 
                 mouse 
                 IgG1 
                 1D11.16.8 
                 HB-9849 †   
               
               
                 Theophylline 
                 mouse 
                 IgG1; kappa 
                 17/14 
                 HB-8153 †   
               
               
                 Theophylline 
                 mouse 
                 IgG1; kappa 
                 30/15 
                 HB-8152 †   
               
               
                 Theophylline 
                 mouse 
                 IgG1; kappa 
                 61/7 
                 HB-8154 †   
               
               
                 Thy-1 antigen, human 
                 mouse 
                 IgG1 
                 K117 
                 HB-8553 †   
               
               
                 Thy-1 antigen, mouse 
                 mouse 
                 IgM 
                 HO-22-1 
                 TIB-100 
               
               
                 Thy-1 antigen, mouse 
                 rat/mouse 
                 IgG2a 
                 M5/49.4.1 
                 TIB-238 
               
               
                 Thy-1.1 antigen, mouse 
                 mouse 
                 IgM 
                 T11D7e2 
                 TIB-103 
               
               
                 Thy-1.2 antigen, mouse 
                 mouse 
                 IgM 
                 HO-13-4 
                 TIB-99 
               
               
                 Thy-1.2 antigen, mouse 
                 rat/mouse 
                 IgG2b 
                 30-H12 
                 TIB-107 
               
               
                 Thy-1.2 antigen, mouse 
                 rat/mouse 
                 IgM 
                 J1j.10 
                 TIB-184 
               
               
                 Thymic lymphocyte, human 
                 mouse 
                 IgG1 
                 OKT 6 
                 CRL-8020 
               
               
                 Thymocyte (E rosette positive), human 
                 mouse 
                 IgG1 
                 OKT 11 
                 CRL-8027 
               
               
                 Thymocyte, human 
                 mouse 
                 IgG2a 
                 A1G3 
                 HB-177 
               
               
                 Thymocyte, human 
                 mouse 
                 IgG1 
                 OKT 6 
                 CRL-8020 
               
               
                 Thymocytes, rabbit 
                 mouse 
                 IgM 
                 9AE10 
                 CRL-1761 
               
               
                 Thymus, cortical epithelium, human 
                 mouse 
                 IgG2b 
                 CDR2 
                 HB-214 
               
               
                 Thymus, cortical epithelium, human 
                 mouse 
                 IgG2 
                 TE3 
                 HB-209 
               
               
                 Thymus, cortical epithelium, human 
                 mouse 
                 IgM 
                 TE4 
                 HB-207 
               
               
                 Thymus, epithelium, human 
                 mouse 
                 IgM 
                 TE15 
                 HB-206 
               
               
                 Thymus, epithelium, human 
                 mouse 
                 IgG1 
                 TE16 
                 HB-210 
               
               
                 Thymus, epithelium, human 
                 mouse 
                 IgM 
                 TE19 
                 HB-211 
               
               
                 Thymus, epithelium, human 
                 mouse 
                 IgG2a 
                 TE8 
                 HB-212 
               
               
                 Thymus, human 
                 mouse 
                 IgG1 
                 TE7 
                 HB-208 
               
               
                 Thyroid stimulating hormone (TSH) receptor alpha 
                 mouse 
                 IgG1 
                 TSHR-R5T-44 
                 CRL-2681 
               
               
                 subunit, human 
               
               
                 Thyroid stimulating hormone (TSH) receptor alpha 
                 mouse 
                 IgG1 
                 TSHR-T5-51 
                 CRL-2680 
               
               
                 subunit, human 
               
               
                 Thyroid stimulating hormone (TSH) receptor alpha 
                 mouse 
                 IgG1 
                 TSHR-T5U-317 
                 CRL-2682 
               
               
                 subunit, human 
               
               
                 Thyroid stimulating hormone (TSH) receptor beta 
                 mouse 
                 IgG1 
                 TSHR-R5T-34 
                 CRL-2683 
               
               
                 subunit, human 
               
               
                 Thyroid stimulating hormone (TSH) receptor beta 
                 mouse 
                 IgG2a 
                 TSHR-T3-365 
                 CRL-2684 
               
               
                 subunit, human 
               
               
                 L-Thyroxine (T4,3,5,3′,5′-tetraiodo-L-thyronine) 
                 mouse 
                 IgG1 
                 T4 Clone 5 (10-0101, 
                 HB-8500 †   
               
               
                   
                   
                   
                 0062-83) 
               
               
                 Ti1b antigen, human 
                 mouse 
                 IgM 
                 2T8-3E10 
                 HB-8213 †   
               
               
                 TL antigen, mouse 
                 mouse 
                 IgG2a 
                 I(TL.m9) 
                 HB-131 
               
               
                 TL antigen, mouse 
                 rat/mouse 
                 IgG2a 
                 HD168 
                 HB-252 
               
               
                 Transferrin receptor, human 
                 mouse 
                 IgG2a 
                 L5.1 
                 HB-84 
               
               
                 Transferrin receptor, human 
                 mouse 
                 IgG1 
                 OKT 9 
                 CRL-8021 
               
               
                 Transferrin receptor, mouse 
                 rat/mouse 
                 IgM 
                 R17 208.2 
                 TIB-220 
               
               
                 Transferrin receptor, mouse 
                 rat/mouse 
                 IgG2a 
                 R17 217.1.3 
                 TIB-219 
               
               
                 Transforming growth factor-beta2, mouse 
                 mouse 
                 IgG1 
                 1D11.16.8 
                 HB-9849 †   
               
               
                 
                   Treponema pallidum 
                 
                 mouse 
                 IgM 
                 1939-3G5 
                 HB-8133 †   
               
               
                 
                   Treponema pallidum 
                 
                 mouse 
                 IgG1 
                 1939-8G2 
                 HB-8134 †   
               
               
                 
                   Trichinella spiralis 
                 
                 mouse 
                 IgM 
                 7C 2 C 5 C 12   
                 HB-8678 †   
               
               
                 Trifucosylated type 2 chain glycolipids 
                 mouse 
                 IgM 
                 FHCR-1-2075/FH5 
                 HB-8770 †   
               
               
                 2,4,6-Trinitrophenyl (TNP) 
                 mouse 
                 IgG1 
                 1B7.11 
                 TIB-191 
               
               
                 2,4,6-Trinitrophenyl (TNP) 
                 mouse 
                 IgA 
                 2F.11.15 
                 TIB-194 
               
               
                 2,4,6-Trinitrophenyl (TNP) 
                 mouse 
                 IgE (Iga haplotype) 
                 IGEL a2 
                 TIB-142 
               
               
                 2,4,6-Trinitrophenyl (TNP) 
                 mouse 
                 IgE (Igb haplotype) 
                 IGEL b4 
                 TIB-141 
               
               
                 Trop-1, human 
                 mouse 
                 IgG2a 
                 162-21.2 
                 HB-241 
               
               
                 Trop-2, human 
                 mouse 
                 IgG1 (Igh-4a 
                 162-46.2 
                 HB-187 
               
               
                   
                   
                 allotype) 
               
               
                 Trophoblasts, human 
                 mouse 
                 IgG1 (Igh-4a 
                 162-46.2 
                 HB-187 
               
               
                   
                   
                 allotype) 
               
               
                 Tubulin, beta, nematode 
                 mouse 
                 IgG 
                 P3D 
                 HB-11129 †   
               
               
                 Tumor, intracellular antigen, human 
                 human/mouse 
                 IgM 
                 Ch13 
                 HB-8573 †   
               
               
                 Tumor, intracellular antigen, human 
                 human/mouse 
                 IgM 
                 Gr431 
                 HB-8575 †   
               
               
                 Tumor, intracellular antigen, human 
                 human/mouse 
                 IgM 
                 Te39 
                 HB-8577 †   
               
               
                 Tumor-associated glycoprotein (TAG-72), human 
                 mouse 
                 IgG1 kappa 
                 CC 49 
                 HB-9459 †   
               
               
                 Tumors, human 
                 mouse 
                 IgM 
                 B5 NIH 
                 HB-10569 †   
               
               
                 Tumors, neuroectoderm, human 
                 mouse 
                 IgM 
                 PI 153/3 
                 TIB-198 
               
               
                 Uracil DNA glycosylase (UDG), human 
                 mouse 
                 IgM 
                 37.04.12 
                 HB-9312 †   
               
               
                 Uracil DNA glycosylase (UDG), human 
                 mouse 
                 IgG 
                 40.10.09 
                 HB-9311 †   
               
               
                 Uracil DNA glycosylase (UDG), human 
                 mouse 
                 IgM 
                 42.08.07 
                 HB-9313 †   
               
               
                 Uterine carcinoma cell lines, human 
                 mouse 
                 IgM 
                 MH55 
                 HB-8412 †   
               
               
                 VacA (vacuolating cytotoxin) 
                 mouse 
                 IgG1; kappa 
                 5E4 
                 CRL-2635 
               
               
                 VacA (vacuolating cytotoxin) 
                 mouse 
                 IgG1; kappa 
                 5G5 
                 CRL-2633 
               
               
                 VacA (vacuolating cytotoxin) 
                 mouse 
                 IgG1; kappa 
                 B3D 
                 CRL-2634 
               
               
                 Vascular cell adhesion molecule 1, human and 
                 mouse 
                 IgG1; kappa 
                 VIII-6G10 
                 HB-10519 †   
               
               
                 macaque 
               
               
                 Vascular cell adhesion molecule 1, mouse 
                 rat/mouse 
                 IgG1; kappa 
                 M/K-1.9 
                 CRL-1910 
               
               
                 Vascular cell adhesion molecule 1, mouse 
                 rat/mouse 
                 IgG1; kappa 
                 M/K-2.7 
                 CRL-1909 
               
               
                 Vascular endothelial growth factor (VEGF) 
                 rat/mouse 
                 IgG1; kappa 
                 DC101 
                 HB-11534 †   
               
               
                 receptor-2, mouse 
               
               
                 Vasopressin-neurophysin (NP-AVP), rat 
                 mouse 
                 IgG2b; kappa 
                 PS 41 
                 CRL-1799 
               
               
                 Vasopressin-neurophysin (NP-AVP), rat 
                 mouse 
                 IgG2b; kappa 
                 PS 45 
                 CRL-1798 
               
               
                 VCAM-1, human and macaque 
                 mouse 
                 IgG1; kappa 
                 VIII-6G10 
                 HB-10519 †   
               
               
                 VCAM-1, mouse 
                 rat/mouse 
                 IgG1; kappa 
                 M/K-1.9 
                 CRL-1910 
               
               
                 VCAM-1, mouse 
                 rat/mouse 
                 IgG1; kappa 
                 M/K-2.7 
                 CRL-1909 
               
               
                 Very late antigen 1 (VLA-1) alpha, human 
                 mouse 
                 IgG1 
                 TS2/7.1.1 
                 HB-245 
               
               
                 Very late antigen 1 (VLA-1) beta, human 
                 mouse 
                 IgG1 
                 TS2/16.2.1 
                 HB-243 
               
               
                 Very late antigen 4 (VLA-4), mouse 
                 rat/mouse 
                 IgG2b 
                 R1-2 
                 HB-227 
               
               
                 Very late antigen 4 (VLA-4), sheep 
                 mouse 
                 IgG2b 
                 FW3-218-1 
                 HB-261 
               
               
                 Very low density lipoprotein (VLDL) receptor 
                 mouse 
                 IgG1 
                 IgG-6A6 
                 CRL-2197 
               
               
                 Vesicular stomatitis virus surface glycoprotein 
                 mouse 
                 IgG1 
                 11-Hybridoma 
                 CRL-2700 
               
               
                 Vitamin B6 
                 mouse 
                 IgG1 
                 E6(2)2 
                 HB-8172 †   
               
               
                 Vitamin D3 receptor, pig 
                 mouse 
                 IgG1 
                 XVI E6E6G10 
                 HB-9496 †   
               
               
                 Vitonectin receptor (VnR), human 
                 mouse 
                 IgG1 
                 B6H12.2 
                 HB-9771 †   
               
               
                 Vulva, cancer 
                 human 
                 IgG4 
                 VLN3G2 
                 HB-8636 †   
               
               
                 Vulva, cancer 
                 human 
                 IgG 
                 VLN6H2 
                 HB-8633 †   
               
               
                 WC1, bovine 
                 mouse 
                 IgG2a 
                 CC15 
                 HB-265 
               
               
                 WC1, bovine 
                 mouse 
                 IgG1 
                 CC39 
                 HB-274 
               
               
                 WC1, bovine 
                 mouse 
                 IgG1 
                 IL-A29 
                 CRL-1874 
               
               
                 WC3 bovine B cell antigen (BoWC3) 
                 mouse 
                 IgG1 
                 CC21 
                 HB-288 
               
               
                 WC4, bovine 
                 mouse 
                 IgG1 
                 CC55 
                 HB-282 
               
               
                 WC4, bovine 
                 mouse 
                 IgG1 
                 CC57 
                 HB-268 
               
               
                 Yellow fever virus 
                 mouse 
                 IgG2a; kappa 
                 2D12 
                 CRL-1689 
               
               
                   Yersinia pestis , F1 antigen 
                 mouse 
                 IgA 
                 F1-3G8-1 
                 HB-192 
               
               
                 yes (c-yes) oncogene peptide, synthetic 
                 mouse 
                 IgG1 
                 240-13D10 
                 CRL-2672 
               
               
                 ZP1 glycoprotein, mouse 
                 rat/mouse 
                 IgG2a 
                 M1.4 
                 CRL-2464 
               
               
                 ZP2 glycoprotein, human 
                 mouse 
                 IgG1; kappa 
                 H2.8 
                 CRL-2568 
               
               
                 ZP2 glycoprotein, mouse 
                 rat/mouse 
                 IgG2a 
                 IE-3 
                 CRL-2463 
               
               
                 ZP3 glycoprotein, human 
                 mouse 
                 IgG1; kappa 
                 H3.1 
                 CRL-2569 
               
               
                 ZP3 glycoprotein, mouse 
                 rat/mouse 
                 IgG2a 
                 IE-10 
                 CRL-2462