Abstract:
The present invention relates to a compound having the general formula (I), optionally in the form of a pharmaceutically acceptable salt, solvate, polymorph, prodrug, tautomer, racemate, enantiomer, or diastereomer or mixture thereof, 
     
       
                 
         
             
             
         
       
     
     which is useful in treating, ameliorating or preventing a viral disease. Furthermore, specific combination therapies are disclosed.

Description:
CROSS-REFERENCE TO RELATED APPLICATIONS 
       [0001]    This application claims priority from U.S. Provisional Patent Application No. 62/220,817, filed Sep. 18, 2015, which is incorporated herein by reference in its entirety. 
     
    
     FIELD OF THE INVENTION 
       [0002]    The present invention relates to a compound having the general formula (I), optionally in the form of a pharmaceutically acceptable salt, solvate, polymorph, prodrug, tautomer, racemate, enantiomer, or diastereomer or mixture thereof, 
         [0000]    
       
                 
         
             
             
         
       
     
         [0000]    which is useful in treating, ameliorating or preventing a viral disease. Furthermore, specific combination therapies are disclosed. 
       BACKGROUND OF THE INVENTION 
       [0003]    In recent years the serious threat posed by influenza virus to worldwide public health has been highlighted by, firstly, the ongoing low level transmission to humans of the highly pathogenic avian H5N1 strain (63% mortality in infected humans, http://www.who.int/csr/disease/avian_influenza/en/) and secondly, the unexpected emergence in 2009 of a novel pandemic strain A/H1N1 that has rapidly spread around the entire world (http://www.who.int/csr/disease/swineflu/en/). Whilst the new strain is highly contagious but currently generally only gives mild illness, the future evolution of this virus is unpredictable. In a much more serious, but highly plausible scenario, H5N1 could have been more easily transmissible between humans or the new A/H1N1 could have been more virulent and could have carried the single point mutation that confers Tamiflu resistance (Neumann et al., Nature, 2009 (18; 459(7249) 931-939), as many seasonal H1N1 strains have recently done (Dharan et al., The Journal of the American Medical Association, 2009 Mar. 11; 301 (10), 1034-1041; Moscona et al., The New England Journal of Medicine, 2009 (Mar. 5;360(10) pp 953-956). In this case, the delay in generating and deploying a vaccine (˜6 months in the relatively favourable case of A/H1 N1 and still not a solved problem for H5N1) could have been catastrophically costly in human lives and societal disruption. 
         [0004]    It is widely acknowledged that to bridge the period before a new vaccine becomes available and to treat severe cases, as well as to counter the problem of viral resistance, a wider choice of anti-influenza drugs is required. Development of new anti-influenza drugs has therefore again become a high priority, having been largely abandoned by the major pharmaceutical companies once the anti-neuraminidase drugs became available. 
         [0005]    An excellent starting point for the development of antiviral medication is structural data of essential viral proteins. Thus, the crystal structure determination of e.g. the influenza virus surface antigen neuraminidase (Von Itzstein, M. et al., (1993), Nature, 363, pp. 418-423) led directly to the development of neuraminidase inhibitors with anti-viral activity preventing the release of virus from the cells, however, not the virus production. These and their derivatives have subsequently developed into the anti-influenza drugs, zanamivir (Glaxo) and oseltamivir (Roche), which are currently being stockpiled by many countries as a first line of defence against an eventual pandemic. However, these medicaments only provide a reduction in the duration of the clinical disease. Alternatively, other anti-influenza compounds such as amantadine and rimantadine target an ion channel protein, i.e., the M2 protein, in the viral membrane interfering with the uncoating of the virus inside the cell. However, they have not been extensively used due to their side effects and the rapid development of resistant virus mutants (Magden, J. et al., (2005), Appl. Microbiol. Biotechnol., 66, pp. 612-621). In addition, more unspecific viral drugs, such as ribavirin, have been shown to work for treating of influenza and other virus infections (Eriksson, B. et al., (1977), Antimicrob. Agents Chemother., 11, pp. 946-951). However, ribavirin is only approved in a few countries (Furuta et al., Antimicrobial Agents and Chemotherapy, 2005 March 49(3); 981-986), probably due to severe side effects. Clearly, new antiviral compounds are needed, preferably directed against different targets. 
         [0006]    Influenza virus as well as Thogotovirus belong to the family of Orthomyxoviridae which, as well as the family of the Bunyaviridae, including the Hantavirus, Nairovirus, Orthobunyavirus, and Phlebovirus, are negative stranded RNA viruses. Their genome is segmented and comes in ribonucleoprotein particles that include the RNA dependent RNA polymerase which carries out (i) the initial copying of the single-stranded virion RNA (vRNA) into viral mRNAs and (ii) the vRNA replication. This enzyme, a trimeric complex composed of subunits PA, PB1 and PB2, is central to the life cycle of the virus since it is responsible for the replication and transcription of viral RNA. In previous work the atomic structure of two key domains of the polymerase, the mRNA cap-binding domain in the PB2 subunit (Guilligay et al., Antimicrobial Agents and Chemotherapy, 2005 March 49(3); pp 981-986) and the endonuclease-active site in the PA subunit (Dias et al., Nature 2009; Apr 16;458(7240); 914-918) have been identified and determined. These two sites are critical for the unique cap-snatching mode of transcription that is used by influenza virus to generate viral mRNAs. For the generation of viral mRNA the polymerase makes use of the so called “cap-snatching” mechanism (Plotch, S. J. et al., (1981), Cell, 23, pp. 847-858; Kukkonen, S. K. et al (2005), Arch. Virol., 150, pp. 533-556; Leahy, M. B. et al, (2005), J. Virol., 71, pp. 8347-8351; Noah, D. L. et al., (2005), Adv. Virus Res., 65, pp. 121-145). A 5′ cap (also termed an RNA cap, RNA 7-methylguanosine cap or an RNA m7G cap) is a modified guanine nucleotide that has been added to the 5′ end of each cellular messenger RNA. The 5′RNA cap consists of a terminal 7-methylguanosine residue which is linked through a 5′-5′-triphosphate bond to the first transcribed nucleotide. Upon influenza virus infection the 5′RNA cap of cellular mRNA molecules is bound by the viral polymerase complex, specifically the cap-binding domain within the PB2 subunit of the polymerase complex, and the RNA cap together with a stretch of 10 to 15 nucleotides is cleaved by the viral endonuclease which resides within the PA subunit of the viral polymerase complex. The capped RNA fragments then serve as primers for the synthesis of viral mRNA. 
         [0007]    The cap-binding domain in the PB2 subunit of the viral polymerase has been unequivocally identified and structurally characterized by Guilligay et al., 2008. Binding the capped host cell mRNA via the cap-binding site and hence bringing the host cell mRNA strand into close spatial vicinity of the endonuclease active site is a prerequisite for the endonuclease to snatch off the cap. Therefore the cap-binding site in PB2 is essential for cap-dependent transcription by the viral RNPs and mandatory for the viral replication cycle. This together with the fact that the PB2 cap-binding domain is structually distinct from other cap binding proteins, this suggests that the ligand binding site is a good target for the development of new antiviral drugs. 
         [0008]    Generally, the polymerase complex seems to be an appropriate antiviral drug target since it is essential for synthesis of viral mRNA and viral replication and contains several functional active sites likely to be significantly different from those found in host cell proteins (Magden, J. et al., (2005), Appl. Microbiol. Biotechnol., 66, pp. 612-621). Thus, for example, there have been attempts to interfere with the assembly of polymerase subunits by a 25-amino-acid peptide resembling the PA-binding domain within PB1 (Ghanem, A. et al., (2007), J. Virol., 81, pp. 7801-7804). Furthermore, the endonuclease activity of the polymerase has been targeted and a series of 4-substituted 2,4-dioxobutanoic acid compounds has been identified as selective inhibitors of this activity in influenza viruses (Tomassini, J. et al., (1994), Antimicrob. Agents Chemother., 38, pp. 2827-2837). In addition, flutimide, a substituted 2,6-diketopiperazine, identified in extracts of Delitschia confertaspora, a fungal species, has been shown to inhibit the endonuclease of influenza virus (Tomassini, J. et al., (1996), Antimicrob. Agents Chemother., 40, pp. 1189-1193). Moreover, there have been attempts to interfere with viral transcription by nucleoside analogs, such as 2′-deoxy-2′-fluoroguanosine (Tisdale, M. et al., (1995), Antimicrob. Agents Chemother., 39, pp. 2454-2458). 
         [0009]    It is an object of the present invention to identify compounds which specifically target the influenza virus cap-binding domain and hence are effective against viral diseases and which have improved pharmacological properties. 
     
    
     
       SHORT DESCRIPTION OF THE FIGURE 
         [0010]    
         FIG. 1 
       
           [0011]    Sequence of the de novo synthesized viral mRNA used for Quantigene TA assay probe set design: Label Extenders (LE) hybridize to the capped primer sequence derived from provided synthetic RNA substrate and first bases of the de novo synthesized viral mRNA at the 5′-end (LE1), and to the poly a tail at the 3′-end (LE2). Capture Extenders (CE1-9) specifically hybridize to gene specific regions and concomitantly immobilize the captured RNA to the plate. Blocking Probes (BP) hybridize to different stretches of the de novo synthesized viral mRNA. The sequence shown in italics at the 3′-end was verified by 3′-RLM RACE (not complete sequence shown). The probe sets are supplied as a mix of all three by Panomics. 
         SUMMARY OF THE INVENTION 
         [0012]    Accordingly, in a first embodiment, the present invention provides a compound having the general formula (I). These compounds are suitable for use in the treatment, amelioration or prevention of a viral disease. 
           [0000]    
         
                   
           
               
               
           
         
       
           [0013]    It is understood that throughout the present specification the term “a compound having the general formula (I)” encompasses pharmaceutically acceptable salts, solvates, polymorphs, prodrugs, tautomers, racemates, enantiomers, or diastereomers or mixtures thereof unless mentioned otherwise. 
       
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
       [0014]    Before the present invention is described in detail below, it is to be understood that this invention is not limited to the particular methodology, protocols and reagents described herein as these may vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only, and is not intended to limit the scope of the present invention which will be limited only by the appended claims. Unless defined otherwise, all technical and scientific terms used herein have the same meanings as commonly understood by one of ordinary skill in the art. 
         [0015]    Preferably, the terms used herein are defined as described in “A multilingual glossary of biotechnological terms: (IUPAC Recommendations)”, Leuenberger, H. G. W, Nagel, B. and Kölbl, H. eds. (1995), Helvetica Chimica Acta, CH-4010 Basel, Switzerland). 
         [0016]    Throughout this specification and the claims which follow, unless the context requires otherwise, the word “comprise”, and variations such as “comprises” and “comprising”, will be understood to imply the inclusion of a stated integer or step or group of integers or steps but not the exclusion of any other integer or step or group of integers or steps. In the following passages different aspects of the invention are defined in more detail. Each aspect so defined may be combined with any other aspect or aspects unless clearly indicated to the contrary. In particular, any feature indicated as being preferred or advantageous may be combined with any other feature or features indicated as being preferred or advantageous. 
         [0017]    Several documents are cited throughout the text of this specification. Each of the documents cited herein (including all patents, patent applications, scientific publications, manufacturer&#39;s specifications, instructions, etc.), whether supra or infra, are hereby incorporated by reference in their entirety. Nothing herein is to be construed as an admission that the invention is not entitled to antedate such disclosure by virtue of prior invention. 
         [0018]    Definitions 
         [0019]    The term “alkyl” refers to a saturated straight or branched or cyclic hydrocarbon group. Preferably, the term “alkyl” refers to a saturated straight or branched hydrocarbon group. 
         [0020]    “Hal” of “halogen” represents F, Cl, Br and I. 
         [0021]    The term “heterocyclyl” covers any ring or ring system having the indicated number of ring atoms, wherein at least one of the carbon atoms in the ring (system) has been replaced a heteroatom. If more than one heteroatom is present, they can be the same or different. The heteroatoms are preferably selected from O, N and S. The term “heterocyclyl” also covers heteroaryl rings. The term covers monocyclic rings as well as fused ring systems. 
         [0022]    Examples of monocyclic rings include pyrrolidine; pyrrole; tetrahydrofuran; furan; thiolane; thiophene; imidazolidine; pyrazolidine; imidazole; imidazoline; pyrazole; pyrazoline; oxazolidine; isoxazolidine; oxazole; oxazoline; isoxazole; thiazolidine; isothiazolidine; thiazole; thiazoline; isothiazole; dioxolane; dithiolane; triazoles; furazan; oxadiazole; thiadiazole; dithiazole; tetrazole; piperidine; pyridine; oxane; pyran; thiane; thiopyran; piperazine; diazines; morpholine; oxazine; thiomorpholine; thiazine; dioxane; dioxine; dithiane; dithiine; triazine; trioxane; trithiane; tetrazine; azepane; azepine; oxepane; oxepine; thiepane; thiepine; homopiperazine; diazepine; and thiazepine. Fused ring systems can be envisaged as a combination of more than one of the above-mentioned monocyclic heterocyclic rings or as a combination of at least one of the above-mentioned monocyclic heterocyclic ring and a carbocyclic ring. 
         [0023]    The term “heteroaryl” preferably refers to an aromatic ring wherein at least one of the carbon atoms in the ring (system) has been replaced a heteroatom. If more than one heteroatom is present, they can be the same or different. The heteroatoms are preferably selected from O, N and S. Examples of the heteroaryl group can be found in the list of “heterocyclyl” given above. 
         [0024]    The term “carbocyclyl” covers any ring or ring system having the indicated number of ring atoms, which does not include heteroatoms in the ring. The term “carbocyclyl” also covers cycloalkyl and aryl rings. 
         [0025]    The term “cycloalkyl” represents a cyclic version of “alkyl”. 
         [0026]    The term “aryl” preferably refers to an aromatic ring. Examples include phenyl. 
         [0027]    If a compound or moiety is referred to as being “optionally substituted” it can in each instance include 1 or more of the indicated substituents, whereby the substituents can be the same or different. 
         [0028]    The term “pharmaceutically acceptable salt” refers to a salt of a compound of the present invention. Suitable pharmaceutically acceptable salts include acid addition salts which may, for example, be formed by mixing a solution of compounds of the present invention with a solution of a pharmaceutically acceptable acid such as hydrochloric acid, sulfuric acid, fumaric acid, maleic acid, succinic acid, acetic acid, benzoic acid, citric acid, tartaric acid, carbonic acid or phosphoric acid. Furthermore, where the compound carries an acidic moiety, suitable pharmaceutically acceptable salts thereof may include alkali metal salts (e.g., sodium or potassium salts); alkaline earth metal salts (e.g., calcium or magnesium salts); and salts formed with suitable organic ligands (e.g., ammonium, quaternary ammonium and amine cations formed using counteranions such as halide, hydroxide, carboxylate, sulfate, phosphate, nitrate, alkyl sulfonate and aryl sulfonate). Illustrative examples of pharmaceutically acceptable salts include, but are not limited to, acetate, adipate, alginate, ascorbate, aspartate, benzenesulfonate, benzoate, bicarbonate, bisulfate, bitartrate, borate, bromide, butyrate, calcium edetate, camphorate, camphorsulfonate, camsylate, carbonate, chloride, citrate, clavulanate, cyclopentanepropionate, digluconate, dihydrochloride, dodecylsulfate, edetate, edisylate, estolate, esylate, ethanesulfonate, formate, fumarate, gluceptate, glucoheptonate, gluconate, glutamate, glycerophosphate, glycolylarsanilate, hemisulfate, heptanoate, hexanoate, hexylresorcinate, hydrabamine, hydrobromide, hydrochloride, hydroiodide, 2-hydroxy-ethanesulfonate, hydroxynaphthoate, iodide, isothionate, lactate, lactobionate, laurate, lauryl sulfate, malate, maleate, malonate, mandelate, mesylate, methanesulfonate, methylsulfate, mucate, 2-naphthalenesulfonate, napsylate, nicotinate, nitrate, N-methylglucamine ammonium salt, oleate, oxalate, pamoate (embonate), palmitate, pantothenate, pectinate, persulfate, 3-phenylpropionate, phosphate/diphosphate, picrate, pivalate, polygalacturonate, propionate, salicylate, stearate, sulfate, subacetate, succinate, tannate, tartrate, teoclate, tosylate, triethiodide, undecanoate, valerate, and the like (see, for example, S. M. Berge et al., “Pharmaceutical Salts”, J. Pharm. Sci., 66, pp. 1-19 (1977)). 
         [0029]    When the compounds of the present invention are provided in crystalline form, the structure can contain solvent molecules. The solvents are typically pharmaceutically acceptable solvents and include, among others, water (hydrates) or organic solvents. Examples of possible solvates include ethanolates and iso-propanolates. 
         [0030]    The compounds of the present invention can also be provided in the form of a prodrug, namely a compound which is metabolized in vivo to the active metabolite. 
       Compounds having the General Formula (I) 
       [0031]    The present invention provides a compound having the general formula (I): 
         [0000]    
       
                 
         
             
             
         
       
     
         [0032]    The present invention provides a compound having the general formula (I) in which the following definitions apply. 
         [0033]    R 31  is selected from —H, and -(optionally substituted C 1-6  alkyl); preferably R 31  is selected from —H and —C 1-6  alkyl; more preferably R 31  is —H. 
         [0034]    R 36  is selected from —H, -(optionally substituted C 1-6  alkyl), -(optionally substituted C 3-7  carbocyclyl), —C 1-4  alkyl-(optionally substituted C 3-7  carbocyclyl), -(optionally substituted heterocyclyl having 3 to 7 ring atoms), and —C 1-4  alkyl-(optionally substituted heterocyclyl having 3 to 7 ring atoms); preferably R 36  is selected from —H, and —(C 1-6  alkyl); more preferably R 36  is —H. 
         [0035]    R 38  is selected from —H, -(optionally substituted C 1-6  alkyl), -(optionally substituted C 3-7  carbocyclyl), —C 1-4  alkyl-(optionally substituted C 3-7  carbocyclyl), -(optionally substituted heterocyclyl having 3 to 7 ring atoms), and —C 1-4  alkyl-(optionally substituted heterocyclyl having 3 to 7 ring atoms); preferably R 38  is selected from —H, -(optionally substituted C 1-6  alkyl), -(optionally substituted C 3-7  carbocyclyl), and -(optionally substituted heterocyclyl having 3 to 7 ring atoms). More preferably R 38  is selected from —H, -(optionally substituted C 1-6  alkyl), -(optionally substituted C 5-6  carbocyclyl), and -(optionally substituted heterocyclyl having 5 to 6 ring atoms). 
         [0036]    R 39  is selected from a -(optionally substituted C 1-6  alkyl), -(optionally substituted C 3-9  carbocyclyl), —C 1-4  alkyl-(optionally substituted C 3-9  carbocyclyl), -(optionally substituted heterocyclyl having 3 to 9 ring atoms), and —C 1-4  alkyl-(optionally substituted heterocyclyl having 3 to 9 ring atoms), wherein the alkyl group can be saturated or unsaturated. In one embodiment, R 39  is selected from a saturated, linear or branched C 1-6  alkyl, wherein the alkyl can be optionally substituted with one or more substituents which are independently selected from —(CH 2 ) s —X 32 —R 38 , —C 3-7  carbocyclyl, —halogen, and —CN. In another embodiment, R 39  is selected from an -(optionally substituted C 3-9  carbocyclyl), wherein the carbocyclyl group can be optionally substituted with one or more substituents which are independently selected from —(CH 2 ) s —X 32 —R 38 , -halogen, and —CN. 
         [0037]    X 32  is selected from NR 36 , N(R 36 )C(O), C(O)NR 36 , O, C(O), C(O)O, OC(O); N(R 36 )SO 2 , SO 2 N(R 36 ), S, SO, and SO 2 ; preferably X 32  is selected from N(R 36 )C(O), C(O)NR 36 , O, C(O), C(O)O, and OC(O); more preferably X 32  is selected from N(R 36 )C(O), C(O)NR 36 , O, C(O)O, and OC(O). 
         [0038]    Hal is a halogen; preferably Hal is F. 
         [0039]    s is 0 to 4; preferably s is 0. 
         [0040]    The alkyl group can be optionally substituted with one or more substituents which are independently selected from —(CH 2 ) s —X 32 —R 38 , C 3-7  carbocyclyl, -(heterocyclyl having 3 to 7 ring atoms), -halogen, —CN, and —CF 3 ; preferably the alkyl group can be optionally substituted with one or more substituents which are independently selected from —(CH 2 ) s —X 32 —R 38 , and —C 3-7  carbocyclyl. 
         [0041]    The carbocyclyl group can be optionally substituted with one or more substituents which are independently selected from —(CH 2 ) s —X 32 —R 38 , -halogen, —CN, —CF 3 , —C 1-6  alkyl, —C 3-7  carbocyclyl which is optionally substituted by —OH or -Hal, —C 1-4  alkyl—C 3-7  carbocyclyl which is optionally substituted by —OH or -Hal, -(heterocyclyl having 3 to 7 ring atoms which is optionally substituted by —OH or -Hal), and —C 1-4  alkyl-(heterocyclyl having 3 to 7 ring atoms which is optionally substituted by —OH or -Hal). In one embodiment, the carbocyclyl group can be optionally substituted with one or more substituents which are independently selected from —(CH 2 ) s —X 32 —R 38 . 
         [0042]    The heterocyclyl group can be optionally substituted with one or more substituents which are independently selected from —(CH 2 ) s —X 32 —R 38 , -halogen, —CN, —CF 3 , —C 1-6  alkyl, —C 3-7  carbocyclyl which is optionally substituted by —OH or -Hal, —C 1-4  alkyl—C 3-7  carbocyclyl which is optionally substituted by —OH or -Hal, -(heterocyclyl having 3 to 7 ring atoms which is optionally substituted by —OH or -Hal), and —C 1-4  alkyl-(heterocyclyl having 3 to 7 ring atoms which is optionally substituted by —OH or -Hal). In one embodiment, the heterocyclyl group can be optionally substituted with one or more substituents which are independently selected from —(CH 2 ) s —X 32 —R 38 . 
         [0043]    The compounds of the present invention can be administered to a patient in the form of a pharmaceutical composition which can optionally comprise one or more pharmaceutically acceptable excipient(s) and/or carrier(s). 
         [0044]    The compounds of the present invention can be administered by various well known routes, including oral, rectal, intragastrical, intracranial and parenteral administration, e.g. intravenous, intramuscular, intranasal, intradermal, subcutaneous, and similar administration routes. Oral, intranasal and parenteral administration are particularly preferred. Depending on the route of administration different pharmaceutical formulations are required and some of those may require that protective coatings are applied to the drug formulation to prevent degradation of a compound of the invention in, for example, the digestive tract. 
         [0045]    Thus, preferably, a compound of the invention is formulated as a syrup, an infusion or injection solution, a spray, a tablet, a capsule, a capslet, lozenge, a liposome, a suppository, a plaster, a band-aid, a retard capsule, a powder, or a slow release formulation. Preferably the diluent is water, a buffer, a buffered salt solution or a salt solution and the carrier preferably is selected from the group consisting of cocoa butter and vitebesole. 
         [0046]    Particular preferred pharmaceutical forms for the administration of a compound of the invention are forms suitable for injectionable use and include sterile aqueous solutions or dispersions and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersion. In all cases the final solution or dispersion form must be sterile and fluid. Typically, such a solution or dispersion will include a solvent or dispersion medium, containing, for example, water-buffered aqueous solutions, e.g. biocompatible buffers, ethanol, polyol, such as glycerol, propylene glycol, polyethylene glycol, suitable mixtures thereof, surfactants or vegetable oils. A compound of the invention can also be formulated into liposomes, in particular for parenteral administration. Liposomes provide the advantage of increased half life in the circulation, if compared to the free drug and a prolonged more even release of the enclosed drug. 
         [0047]    Sterilization of infusion or injection solutions can be accomplished by any number of art recognized techniques including but not limited to addition of preservatives like anti-bacterial or anti-fungal agents, e.g. parabene, chlorobutanol, phenol, sorbic acid or thimersal. Further, isotonic agents, such as sugars or salts, in particular sodium chloride may be incorporated in infusion or injection solutions. 
         [0048]    Production of sterile injectable solutions containing one or several of the compounds of the invention is accomplished by incorporating the respective compound in the required amount in the appropriate solvent with various ingredients enumerated above as required followed by sterilization. To obtain a sterile powder the above solutions are vacuum-dried or freeze-dried as necessary. Preferred diluents of the present invention are water, physiological acceptable buffers, physiological acceptable buffer salt solutions or salt solutions. Preferred carriers are cocoa butter and vitebesole. Excipients which can be used with the various pharmaceutical forms of a compound of the invention can be chosen from the following non-limiting list: 
         [0049]    a) binders such as lactose, mannitol, crystalline sorbitol, dibasic phosphates, calcium phosphates, sugars, microcrystalline cellulose, carboxymethyl cellulose, hydroxyethyl cellulose, polyvinyl pyrrolidone and the like; 
         [0050]    b) lubricants such as magnesium stearate, talc, calcium stearate, zinc stearate, stearic acid, hydrogenated vegetable oil, leucine, glycerids and sodium stearyl fumarates, 
         [0051]    c) disintegrants such as starches, croscaramellose, sodium methyl cellulose, agar, bentonite, alginic acid, carboxymethyl cellulose, polyvinyl pyrrolidone and the like. 
         [0052]    In one embodiment the formulation is for oral administration and the formulation comprises one or more or all of the following ingredients: pregelatinized starch, talc, povidone K 30, croscarmellose sodium, sodium stearyl fumarate, gelatin, titanium dioxide, sorbitol, monosodium citrate, xanthan gum, titanium dioxide, flavoring, sodium benzoate and saccharin sodium. 
         [0053]    If a compound of the invention is administered intranasally in a preferred embodiment, it may be administered in the form of a dry powder inhaler or an aerosol spray from a pressurized container, pump, spray or nebulizer with the use of a suitable propellant, e.g., dichlorodifluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, a hydrofluoro-alkane such as 1,1,1,2-tetrafluoroethane (HFA 134A™) or 1,1,1,2,3,3,3-heptafluoropropane (HFA 227EA™), carbon dioxide, or another suitable gas. The pressurized container, pump, spray or nebulizer may contain a solution or suspension of the compound of the invention, e.g., using a mixture of ethanol and the propellant as the solvent, which may additionally contain a lubricant, e.g., sorbitan trioleate. 
         [0054]    Other suitable excipients can be found in the Handbook of Pharmaceutical Excipients, published by the American Pharmaceutical Association, which is herein incorporated by reference. 
         [0055]    It is to be understood that depending on the severity of the disorder and the particular type which is treatable with one of the compounds of the invention, as well as on the respective patient to be treated, e.g. the general health status of the patient, etc., different doses of the respective compound are required to elicit a therapeutic or prophylactic effect. The determination of the appropriate dose lies within the discretion of the attending physician. It is contemplated that the dosage of a compound of the invention in the therapeutic or prophylactic use of the invention should be in the range of about 0.1 mg to about 1 g of the active ingredient (i.e. compound of the invention) per kg body weight. However, in a preferred use of the present invention a compound of the invention is administered to a subject in need thereof in an amount ranging from 1.0 to 500 mg/kg body weight, preferably ranging from 1 to 200 mg/kg body weight. The duration of therapy with a compound of the invention will vary, depending on the severity of the disease being treated and the condition and idiosyncratic response of each individual patient. In one preferred embodiment of a prophylactic or therapeutic use, between 100 mg to 200 mg of the compound is orally administered to an adult per day, depending on the severity of the disease and/or the degree of exposure to disease carriers. 
         [0056]    As is known in the art, the pharmaceutically effective amount of a given composition will also depend on the administration route. In general the required amount will be higher, if the administration is through the gastrointestinal tract, e.g., by suppository, rectal, or by an intragastric probe, and lower if the route of administration is parenteral, e.g., intravenous. Typically, a compound of the invention will be administered in ranges of 50 mg to 1 g/kg body weight, preferably 100 mg to 500 mg/kg body weight, if rectal or intragastric administration is used and in ranges of 10 to 100 mg/kg body weight, if parenteral administration is used. 
         [0057]    If a person is known to be at risk of developing a disease treatable with a compound of the invention, prophylactic administration of the biologically active blood serum or the pharmaceutical composition according to the invention may be possible. In these cases the respective compound of the invention is preferably administered in above outlined preferred and particular preferred doses on a daily basis. Preferably, from 0.1 mg to 1 g/kg body weight once a day, preferably 10 to 200 mg/kg body weight. This administration can be continued until the risk of developing the respective viral disorder has lessened. In most instances, however, a compound of the invention will be administered once a disease/disorder has been diagnosed. In these cases it is preferred that a first dose of a compound of the invention is administered one, two, three or four times daily. 
         [0058]    The compounds of the present invention are particularly useful for treating, ameliorating, or preventing viral diseases. The type of viral disease is not particularly limited. Examples of possible viral diseases include, but are not limited to, viral diseases which are caused by Poxviridae, Herpesviridae, Adenoviridae, Papillomaviridae, Polyomaviridae, Parvoviridae, Hepadnaviridae, Retroviridae, Reoviridae, Filoviridae, Paramyxoviridae, Rhabdoviridae, Orthomyxoviridae, Bunyaviridae, Arenaviridae, Coronaviridae, Picornaviridae, Hepeviridae, Caliciviridae, Astroviridae, Togaviridae, Flaviviridae, Deltavirus, Bornaviridae, and prions. Preferably viral diseases which are caused by Herpesviridae, Retroviridae, Filoviridae, Paramyxoviridae, Rhabdoviridae, Orthomyxoviridae, Bunyaviridae, Arenaviridae, Coronaviridae, Picornaviridae, Togaviridae, Flaviviridae, more preferably viral diseases which are caused by orthomyxoviridae. 
         [0059]    Examples of the various viruses are given in the following table. 
         [0000]    
       
         
               
               
               
             
           
               
                   
                   
               
               
                   
                 Family 
                 Virus (preferred examples) 
               
               
                   
                   
               
             
             
               
                   
                 Poxviridae 
                 Smallpox virus 
               
               
                   
                   
                 Molluscum contagiosum virus 
               
               
                   
                 Herpesviridae 
                 Herpes simplex virus 
               
               
                   
                   
                 Varicella zoster virus 
               
               
                   
                   
                 Cytomegalovirus 
               
               
                   
                   
                 Epstein Barr virus 
               
               
                   
                   
                 Kaposi&#39;s sarcoma-associated 
               
               
                   
                   
                 herpesvirus 
               
               
                   
                 Adenoviridae 
                 Human adenovirus A-F 
               
               
                   
                 Papillomaviridae 
                 Papillomavirus 
               
               
                   
                 Polyomaviridae 
                 BK-virus 
               
               
                   
                   
                 JC-Virsu 
               
               
                   
                 Parvoviridae 
                 B19 virus 
               
               
                   
                   
                 Adeno associated virus 2/3/5 
               
               
                   
                 Hepadnaviridae 
                 Hepatitis B virus 
               
               
                   
                 Retroviridae 
                 Human immunodeficiency virus 
               
               
                   
                   
                 types 1/2 
               
               
                   
                   
                 Human T-cell leukemia virus 
               
               
                   
                   
                 Human foamy virus 
               
               
                   
                 Reoviridae 
                 Reovirus 1/2/3 
               
               
                   
                   
                 Rotavirus A/B/C 
               
               
                   
                   
                 Colorado tick fever virus 
               
               
                   
                 Filoviridae 
                 Ebola virus 
               
               
                   
                   
                 Marburg virus 
               
               
                   
                 Paramyxoviridae 
                 Parainfluenza virus 1-4 
               
               
                   
                   
                 Mumps virus 
               
               
                   
                   
                 Measles virus 
               
               
                   
                   
                 Respiratory syncytial virus 
               
               
                   
                   
                 Hendravirus 
               
               
                   
                 Rhabdoviridae 
                 Vesicular stomatitis virus 
               
               
                   
                   
                 Rabies virus 
               
               
                   
                   
                 Mokola virus 
               
               
                   
                   
                 European bat virus 
               
               
                   
                   
                 Duvenhage virus 
               
               
                   
                 Orthomyxoviridae 
                 Influenza virus types A-C 
               
               
                   
                 Bunyaviridae 
                 California encephalitis virus 
               
               
                   
                   
                 La Crosse virus 
               
               
                   
                   
                 Hantaan virus 
               
               
                   
                   
                 Puumala virus 
               
               
                   
                   
                 Sin Nombre virus 
               
               
                   
                   
                 Seoul virus 
               
               
                   
                   
                 Crimean- Congo hemorrhagic fever virus 
               
               
                   
                   
                 Sakhalin virus 
               
               
                   
                   
                 Rift valley virus 
               
               
                   
                   
                 Sandfly fever virus 
               
               
                   
                   
                 Uukuniemi virus 
               
               
                   
                 Arenaviridae 
                 Lassa virus 
               
               
                   
                   
                 Lymphocytic choriomeningitis virus 
               
               
                   
                   
                 Guanarito virus 
               
               
                   
                   
                 Junin virus, 
               
               
                   
                   
                 Machupo virus 
               
               
                   
                   
                 Sabia virus 
               
               
                   
                 Coronaviridae 
                 Human coronavirus 
               
               
                   
                 Picornaviridae 
                 Human enterovirus types A-D (Poliovirus, 
               
               
                   
                   
                 Echovirus, Coxsackie virus A/B) 
               
               
                   
                   
                 Rhinovirus types A/B/C 
               
               
                   
                   
                 Hepatitis A virus 
               
               
                   
                   
                 Parechovirus 
               
               
                   
                   
                 Food and mouth disease virus 
               
               
                   
                 Hepeviridae 
                 Hepatitis E virus 
               
               
                   
                 Caliciviridae 
                 Norwalk virus 
               
               
                   
                   
                 Sapporo virus 
               
               
                   
                 Astroviridae 
                 Human astrovirus 1 
               
               
                   
                 Togaviridae 
                 Ross River virus 
               
               
                   
                   
                 Chikungunya virus 
               
               
                   
                   
                 O&#39;nyong-nyong virus 
               
               
                   
                   
                 Rubella virus 
               
               
                   
                 Flaviviridae 
                 Tick-borne encephalitis virus 
               
               
                   
                   
                 Dengue virus 
               
               
                   
                   
                 Yellow Fever virus 
               
               
                   
                   
                 Japanese encephalitis virus 
               
               
                   
                   
                 Murray Valley virus 
               
               
                   
                   
                 St. Louis encephalitis virus 
               
               
                   
                   
                 West Nile virus 
               
               
                   
                   
                 Hepatitis C virus 
               
               
                   
                   
                 Hepatitis G virus 
               
               
                   
                   
                 Hepatitis GB virus 
               
               
                   
                 Deltavirus 
                 Hepatitis deltavirus 
               
               
                   
                 Bornaviridae 
                 Bornavirus 
               
               
                   
                 Prions 
               
               
                   
                   
               
             
          
         
       
     
         [0060]    Preferably the compounds of the present invention are employed to treat influenza. Within the present invention, the term “influenza” includes influenza A, B, C, isavirus and thogotovirus and also covers bird flu and swine flu. The subject to be treated is not particularly restricted and can be any vertebrate, such as birds and mammals (including humans). 
         [0061]    Without wishing to be bound by theory it is assumed that the compounds of the present invention are capable of inhibiting binding of host mRNA cap structures to the cap-binding domain (CBD), particularly of the influenza virus. More specifically it is assumed that they directly interfere with the CBD of the influenza PB2 protein. However, delivery of a compound into a cell may represent a problem depending on, e.g., the solubility of the compound or its capabilities to cross the cell membrane. The present invention not only shows that the claimed compounds have in vitro polymerase inhibitory activity but also cellular antiviral activity. 
         [0062]    A possible measure of the in vivo antiviral activity of the compounds having the formula I or (I) is the CPE assay disclosed herein. Preferably the compounds exhibit a % reduction of at least about 30% at 50 μM. In this connection, the reduction in the virus-mediated cytopathic effect (CPE) upon treatment with the compounds was calculated as follows: The cell viability of infected-treated and uninfected-treated cells was determined using an ATP-based cell viability assay (Promega). The response in relative luminescent units (RLU) of infected-untreated samples was subtracted from the response (RLU) of the infected-treated samples and then normalized to the viability of the corresponding uninfected sample resulting in % CPE reduction. Preferably the compounds exhibit an IC 50  of at least about 45 μM, more preferably at least about 10 μM, in the CPE assay. The half maximal inhibitory concentration (IC 50 ) is a measure of the effectiveness of a compound in inhibiting biological or biochemical function and was calculated from the RLU response in a given concentration series ranging from maximum 100 μM to at least 100 nM. 
         [0063]    The activity of the compounds having the formula (I) can also be measured by the Cap Fluorescence-Polarization Ligand Displacement (CapFP-LD) assay as disclosed herein. 
         [0064]    The compounds having the general formula (I) can be used in combination with one or more other medicaments. The type of the other medicaments is not particularly limited and will depend on the disorder to be treated. Preferably the other medicament will be a further medicament which is useful in treating, ameliorating or preventing a viral disease, more preferably a further medicament which is useful in treating, ameliorating or preventing influenza. 
         [0065]    The following combinations of medicaments are envisaged as being particularly suitable:
   (i) The combination of endonuclease and cap binding inhibitors (particularly targeting influenza). The endonuclease inhibitors are not particularly limited and can be any endonuclease inhibitor, particularly any viral endonuclease inhibitor.
       Widespread resistance to both classes of licensed influenza antivirals (M2 ion channel inhibitors (adamantanes) and neuraminidase inhibitors (Oseltamivir)) occurs in both pandemic and seasonal viruses, rendering these drugs to be of marginal utility in the treatment modality. For M2 ion channel inhibitors, the frequency of viral resistance has been increasing since 2003 and for seasonal influenza A/H3N2, adamantanes are now regarded as ineffective. Virtually all 2009 H1N1 and seasonal H3N2 strains are resistant to the adamantanes (rimantadine and amantadine), and the majority of seasonal H1N1 strains are resistant to oseltamivir, the most widely prescribed neuraminidase inhibitor (NAI). For oseltamivir the WHO reported on significant emergence of influenza A/H1N1 resistance starting in the influenza season 2007/2008; and for the second and third quarters of 2008 in the southern hemisphere. Even more serious numbers were published for the fourth quarter of 2008 (northern hemisphere) where 95% of all tested isolates revealed no Oseltamivir-susceptibility. Considering the fact that now most national governments have been stockpiling Oseltamivir as part of their influenza pandemic preparedness plan, it is obvious that the demand for new, effective drugs is growing significantly. To address the need for more effective therapy, preliminary studies using double or even triple combinations of antiviral drugs with different mechanisms of action have been undertaken. Adamantanes and neuraminidase inhibitors in combination were analysed in vitro and in vivo and found to act highly synergistically. However, it is known that for both types of antivirals resistant viruses emerge rather rapidly and this issue is not tackled by combining these established antiviral drugs.   Influenza virus polymerase inhibitors are novel drugs targeting the transcription activity of the polymerase. Selective inhibitors against the cap-binding and endonuclease active sites of the viral polymerase severely attenuate virus infection by stopping the viral reproductive cycle. These two targets are located within distinct subunits of the polymerase complex and thus represent unique drug targets. Due to the fact that both functions are required for the so-called “cap-snatching” mechanism mandatory for viral transcription, concurrent inhibition of both functions is expected to act highly synergistically. This highly efficient drug combination would result in lower substance concentrations and hence improved dose-response-relationships and better side effect profiles.   Both of these active sites are composed of identical residues in all influenza A strains (e.g., avian and human) and hence this high degree of sequence conservation underpins the perception that these targets are not likely to trigger rapid resistant virus generation. Thus, endonuclease and cap-binding inhibitors individually and in combination are ideal drug candidates to combat both seasonal and pandemic influenza, irrespectively of the virus strain.   The combination of an endonuclease inhibitor and a cap-binding inhibitor or a dual specific polymerase inhibitor targeting both the endonuclease active site and the cap-binding domain would be effective against virus strains resistant against adamantanes and neuraminidase inhibitors and moreover combine the advantage of low susceptibility to resistance generation with activity against a broad range of virus strains.   
       (ii) The combination of inhibitors of different antiviral targets (particularly targeting influenza) focusing on the combination with (preferably influenza) polymerase inhibitors as dual or multiple combination therapy. Influenza virus polymerase inhibitors are novel drugs targeting the transcription activity of the polymerase. Selective inhibitors against the cap-binding and endonuclease active sites of the viral polymerase severely attenuate virus infection by stopping the viral reproductive cycle. The combination of a polymerase inhibitor specifically addressing a viral intracellular target with an inhibitor of a different antiviral target is expected to act highly synergistically. This is based on the fact that these different types of antiviral drugs exhibit completely different mechanisms of action and pharmacokinetics properties which act advantageously and synergistically on the antiviral efficacy of the combination.
       This highly efficient drug combination would result in lower substance concentrations and hence improved dose-response-relationships and better side effect profiles. Moreover, advantages described under (i) for polymerase inhibitors would prevail for combinations of inhibitors of different antiviral targets with polymerase inhibitors.   Typically at least one compound selected from the first group of polymerase inhibitors is combined with at least one compound selected from the second group of polymerase inhibitors.   The first group of polymerase inhibitors which can be used in this type of combination therapy includes, but is not limited to, the compounds having the general formula (I) described below, the compounds having the general formula ((I)) described above and/or the compounds disclosed in WO2011/000566.   The second group of polymerase inhibitors which can be used in this type of combination therapy includes, but is not limited to, compounds disclosed in WO 2010/110231, WO 2010/110409, WO 2006/030807 and U.S. Pat. No. 5,475,109 as well as flutimide and analogues, favipiravir and analogues, epigallocatechin gallate and analogues, as well as nucleoside analogs such as ribavirine.   
       (iii) The combination of polymerase inhibitors with neuramidase inhibitors
       Influenza virus polymerase inhibitors are novel drugs targeting the transcription activity of the polymerase. Selective inhibitors against the cap-binding and endonuclease active sites of the viral polymerase severely attenuate virus infection by stopping the viral reproductive cycle. The combination of a polymerase inhibitor specifically addressing a viral intracellular target with an inhibitor of a different extracellular antiviral target, especially the (e.g., viral) neuraminidase is expected to act highly synergistically. This is based on the fact that these different types of antiviral drugs exhibit completely different mechanisms of action and pharmacokinetic properties which act advantageously and synergistically on the antiviral efficacy of the combination.   This highly efficient drug combination would result in lower substance concentrations and hence improved dose-response-relationships and better side effect profiles. Moreover, advantages described under (i) for polymerase inhibitors would prevail for combinations of inhibitors of different antiviral targets with polymerase inhibitors.   Typically at least one compound selected from the above mentioned first group of polymerase inhibitors is combined with at least one neuramidase inhibitor.   The neuraminidase inhibitor (particularly influenza neuramidase inhibitor) is not specifically limited. Examples include zanamivir, oseltamivir, peramivir, KDN DANA, FANA, and cyclopentane derivatives.   
       (iv) The combination of polymerase inhibitors with M2 channel inhibitors
       Influenza virus polymerase inhibitors are novel drugs targeting the transcription activity of the polymerase. Selective inhibitors against the cap-binding and endonuclease active sites of the viral polymerase severely attenuate virus infection by stopping the viral reproductive cycle. The combination of a polymerase inhibitor specifically addressing a viral intracellular target with an inhibitor of a different extracellular and cytoplasmic antiviral target, especially the viral M2 ion channel, is expected to act highly synergistically. This is based on the fact that these different types of antiviral drugs exhibit completely different mechanisms of action and pharmacokinetic properties which act advantageously and synergistically on the antiviral efficacy of the combination.   This highly efficient drug combination would result in lower substance concentrations and hence improved dose-response-relationships and better side effect profiles. Moreover, advantages described under (i) for polymerase inhibitors would prevail for combinations of inhibitors of different antiviral targets with polymerase inhibitors.   Typically at least one compound selected from the above mentioned first group of polymerase inhibitors is combined with at least one M2 channel inhibitor.   The M2 channel inhibitor (particularly influenza M2 channel inhibitor) is not specifically limited. Examples include amantadine and rimantadine.   
       (v) The combination of polymerase inhibitors with alpha glucosidase inhibitors
       Influenza virus polymerase inhibitors are novel drugs targeting the transcription activity of the polymerase. Selective inhibitors against the cap-binding and endonuclease active sites of the viral polymerase severely attenuate virus infection by stopping the viral reproductive cycle. The combination of a polymerase inhibitor specifically addressing a viral intracellular target, with an inhibitor of a different extracellular target, especially alpha glucosidase, is expected to act highly synergistically. This is based on the fact that these different types of antiviral drugs exhibit completely different mechanisms of action and pharmacokinetic properties which act advantageously and synergistically on the antiviral efficacy of the combination.   This highly efficient drug combination would result in lower substance concentrations and hence improved dose-response-relationships and better side effect profiles. Moreover, advantages described under (i) for polymerase inhibitors would prevail for combinations of inhibitors of different antiviral targets with polymerase inhibitors.   Typically at least one compound selected from the above mentioned first group of polymerase inhibitors is combined with at least one alpha glucosidase inhibitor.   The alpha glucosidase inhibitor (particularly influenza alpha glucosidase inhibitor) is not specifically limited. Examples include the compounds described in Chang et al., Antiviral Research 2011, 89, 26-34.   
       (vi) The combination of polymerase inhibitors with ligands of other influenza targets
       Influenza virus polymerase inhibitors are novel drugs targeting the transcription activity of the polymerase. Selective inhibitors against the cap-binding and endonuclease active sites of the viral polymerase severely attenuate virus infection by stopping the viral reproductive cycle. The combination of a polymerase inhibitor specifically addressing a viral intracellular target with an inhibitor of different extracellular, cytoplasmic or nucleic antiviral targets is expected to act highly synergistically. This is based on the fact that these different types of antiviral drugs exhibit completely different mechanisms of action and pharmacokinetic properties which act advantageously and synergistically on the antiviral efficacy of the combination.   This highly efficient drug combination would result in lower substance concentrations and hence improved dose-response-relationships and better side effect profiles. Moreover, advantages described under (i) for polymerase inhibitors would prevail for combinations of inhibitors of different antiviral targets with polymerase inhibitors.   Typically at least one compound selected from the above mentioned first group of polymerase inhibitors is combined with at least one ligand of another influenza target.   The ligand of another influenza target is not specifically limited. Examples include compounds acting on the sialidase fusion protein, e.g. Fludase (DAS181), siRNAs and phosphorothioate oligonucleotides, signal transduction inhibitors (ErbB tyrosine kinase, Abl kinase family, MAP kinases, PKCa-mediated activation of ERK signaling as well as interferon (inducers).   
       (vii) The combination of (preferably influenza) polymerase inhibitors with a compound used as an adjuvance to minimize the symptoms of the disease (antibiotics, anti-inflammatory agents like COX inhibitors (e.g., COX-1/COX-2 inhibitors, selective COX-2 inhibitors), lipoxygenase inhibitors, EP ligands (particularly EP4 ligands), bradykinin ligands, and/or cannabinoid ligands (e.g., CB2 agonists). Influenza virus polymerase inhibitors are novel drugs targeting the transcription activity of the polymerase. Selective inhibitors against the cap-binding and endonuclease active sites of the viral polymerase severely attenuate virus infection by stopping the viral reproductive cycle. The combination of a polymerase inhibitor specifically addressing a viral intracellular target with an compound used as an adjuvance to minimize the symptoms of the disease address the causative and symptomatic pathological consequences of viral infection. This combination is expected to act synergistically because these different types of drugs exhibit completely different mechanisms of action and pharmacokinetic properties which act advantageously and synergistically on the antiviral efficacy of the combination.
       This highly efficient drug combination would result in lower substance concentrations and hence improved dose-response-relationships and better side effect profiles. Moreover, advantages described under (i) for polymerase inhibitors would prevail for combinations of inhibitors of different antiviral targets with polymerase inhibitors.   
       
 
         [0098]    The present invention not only shows that the compounds have in vitro polymerase inhibitory activity but also in vivo antiviral activity. 
         [0099]    Various modifications and variations of the invention will be apparent to those skilled in the art without departing from the scope of the invention. Although the invention has been described in connection with specific preferred embodiments, it should be understood that the invention as claimed should not be unduly limited to such specific embodiments. Indeed, various modifications of the described modes for carrying out the invention which are obvious to those skilled in the relevant fields are intended to be covered by the present invention. 
         [0100]    The following examples are merely illustrative of the present invention and should not be construed to limit the scope of the invention as indicated by the appended claims in any way. 
       EXAMPLES 
       [0101]    Cytopathic Effect (CPE) Assay 
         [0102]    The influenza A virus (IAV) was obtained from American Tissue Culture Collection (A/Aichi/2/68 (H3N2); VR-547). Virus stocks were prepared by propagation of virus on Mardin-Darby canine kidney (MDCK; ATCC CCL-34) cells and infectious titres of virus stocks were determined by the 50% tissue culture infective dose (TCID 50 ) analysis as described in Reed, L. J., and H. Muench., Am. J. Hyg 1938, 27, 493-497. 
         [0103]    MDCK cells were seeded in 96-well plates at 2x10 4  cells/well using DMEM/Ham&#39;s F-12 (1:1) medium containing 10% foetal bovine serum (FBS), 2 mM L-glutamine and 1% antibiotics (all from PAA). Until infection the cells were incubated for 5 hrs at 37 ° C., 5.0% CO 2  to form a  18  80% confluent monolayer on the bottom of the well. Each test compound was dissolved in DMSO and generally tested at 25 μM and 250 μM. In those cases where the compounds were not soluble at that concentration they were tested at the highest soluble concentration. The compounds were diluted in infection medium (DMEM/Ham&#39;s F-12 (1:1) containing 5 μg/m1 trypsin, and 1% antibiotics) for a final plate well DMSO concentration of 1%. The virus stock was diluted in infection medium (DMEM/Ham&#39;s F-12 (1:1) containing 5 μg/ml Trypsin, 1% DMSO, and 1% antibiotics) to a theoretical multiplicity of infection (MOI) of 0.05. 
         [0104]    After removal of the culture medium and one washing step with PBS, virus and compound were added together to the cells. In the wells used for cytotoxicity determination (i.e. in the absence of viral infection), no virus suspension was added. Instead, infection medium was added. Each treatment was conducted in two replicates. After incubation at 37 ° C., 5% CO 2  for 48 hrs, each well was observed microscopically for apparent cytotoxicity, precipitate formation, or other notable abnormalities. Then, cell viability was determined using CellTiter-Glo luminescent cell viability assay (Promega). The supernatant was removed carefully and 65 μl of the reconstituted reagent were added to each well and incubated with gentle shaking for 15 min at room temperature. Then, 60 μl of the solution was transferred to an opaque plate and luminescence (RLU) was measured using Synergy HT plate reader (Biotek). 
         [0105]    Relative cell viability values of uninfected-treated versus uninfected-untreated cells were used to evaluate cytotoxicity of the compounds. Substances with a relative viability below 80% at the tested concentration were regarded as cytotoxic and retested at lower concentrations. 
         [0106]    Reduction in the virus-mediated cytopathic effect (CPE) upon treatment with the compounds was calculated as follows: The response (RLU) of infected-untreated samples was subtracted from the response (RLU) of the infected-treated samples and then normalized to the viability of the corresponding uninfected sample resulting in % CPE reduction. The half maximal inhibitory concentration (IC 50 ) is a measure of the effectiveness of a compound in inhibiting biological or biochemical function and was calculated from the RLU response in a given concentration series ranging from maximum 100 μM to at least 100 nM. 
         [0107]    Determination of IC 50  Values—Transcription Assay (TA assay) 
         [0108]    TA Assay Principle 
         [0109]    To analyze the activity of the inhibitors, a transcription assay (TA) was employed using the whole RNP complex in a cell-free environment without the use of radioactively labeled nucleotides. 
         [0110]    An in vitro synthesized capped mRNA oligo serves as primer for viral mRNA synthesis as cap-snatching substrate for the viral RNPs and newly synthesized viral mRNA is detected using Quantigene® 2.0 technology. The Quantigene® (QG) technology is based on RNA hybridization bound to coated 96-well plates followed by branched DNA (bDNA) signal amplification. Three different types of probes are responsible for specific hybridization to the gene of interest. The Capture Extenders (CE) hybridize to specific gene regions and concurrently immobilize the RNA to the QG Capture Plate. The Label Extenders (LE) also specifically hybridize to the gene of interest and provide a sequence for the signal amplification tree to be built up via sequential hybridization of preAmplifier (PreAmp), Amplifier (Amp) and alkaline phosphatase Label Probe. The signal is then detected by adding chemiluminescent substrate and using a microplate luminometer for the read out. The third probe blocks nonspecific interactions (Blocking Probe; BP). Generally, probe sets for IAV detection are designed to detect either the negative sense genomic vRNA or synthesized positive sense RNA (+RNA), without differentiating between cRNA or mRNA for translation. For the TA assay, the probe sets and the QG 2.0 protocol were adapted and modified to fit the purpose of a biochemical assay suitable for testing of antiviral compounds in a cell-free environment. 
         [0111]    Materials and Methods 
         [0112]    Compounds 
         [0113]    All compounds were dissolved in DMSO and stored at 4 ° C. All other reagents were obtained from Sigma—Aldrich if not stated otherwise. 
         [0114]    Preparation of RNA Substrate 
         [0115]    The substrate RNA used was derived from in vitro transcribed RNA synthesized by T7 High Yield RNA Synthesis Kit (New England BioLabs Inc.) generated according to the manufacturer&#39;s protocol but with extended incubation time of 16 hr. The RNA product was gel-purified using miRNeasy Mini Kit (Qiagen). The RNA was enzymatically capped using ScriptCap m7G Capping System (CellScript, Madison WI). The resulting capped RNA oligonucleotide (5′-m7GpppG-GGG AAU ACU CAA GCU AUG CAU CGC AUU AGG CAC GUC GAA GUA-3′; SEQ ID NO:1) served as primer for the influenza virus polymerase. 
         [0116]    Preparation of RNPs 
         [0117]    All experiments were done on IAV strain A/PR/8/34, amplified either in embryonated chicken eggs or obtained purified and concentrated from Charles River Laboratories. Egg-amplified virus was PEG-precipitated using 4% w/v PEG8000 in 2mM Tris-HCl (pH 8.0) buffer containing 100 mM NaCl (4° C., 45 min) and centrifuged at 3600 g at 4 ° C. for 45 min. The pellet was suspended in a 10 mM Tris-HCl (pH 8.0) buffer containing 100 mM NaCl and 6% w/v sucrose and was then purified through a 30% w/v sucrose cushion (109,000 g, 120 min, 4 ° C.). 
         [0118]    The RNP purification was performed as previously published with some modifications (Klumpp et al. 2001. Influenza virus endoribonuclease, p. 451-466, 342 ed.). The virus lyophilisate was solved in 1× lysis buffer (1% w/v Triton X-100, 1 mg/mL lysolecithin, 2.5 mM MgCl 2 , 100 mM KCl, 5 mM DTT, 2.5 % v/v glycerol, 20 mM Tris-HCl (pH8.0), 20 U/mL RNase inhibitor) at a final virus protein concentration of 2 mg/mL and was then incubated for 60 minutes at 30 ° C. 3.3mL of the resulting lysate was loaded onto a glycerol gradient (2 mL 70% v/v, 1.5 mL 50% v/v, 0.75 mL 40% v/v and 3.6 mL 33% v/v—buffered in 20 mM Tris-HCl, 50 mM NaCl, 5 mM DTT, 5 mM 2-mercaptoehtanol). The gradients were spun in a Sorvall Ultra centrifuge, AH641 rotor, for 6 hours at 4 ° C. and 240,000 g. Fractions (0.5 mL) were collected from the top of the gradient. The fractions containing the RNP particles were pooled, further concentrated with 10 kD VivaSpin2 columns and stored at -20 ° C. The RNP concentration was determined by UV spectroscopy, using OD260 nm of 1.0=60 mg/mL RNP as conversion factor (Klumpp et al. 2001. Influenza virus endoribonuclease, p. 451-466, 342 ed.). 
         [0119]    RNA Analysis and Transcription Assay (TA Assay) 
         [0120]    All types of viral RNA were analysed by Quantigene® using specific probe sets designed to detect either the negative sense genomic vRNA (−RNA; Cat. No. SF-10318), newly synthesized positive sense RNA (+RNA; Cat. No. SF-10049), or newly synthesized viral mRNA (TA assay; SF-10542) according to the manufacturer&#39;s instructions with the exception that all incubation steps during the Quantigene® procedure were done at 49 ° C. 
         [0121]    For the standard reaction, 80 μM RNPs were incubated for 2 hrs at 30 ° C. with a dilution series of the inhibitors at 1% v/v final DMSO concentration in reaction buffer (55 mM Tris-HCl, 20 mM KCl, 1mM MgCl 2 , 0.2% v/v Triton X-100, 0.25U/μL RNaseOut, 12.5mM NaCl, 1.25 mM DTT, 1.25 mM 2-mercaptoethanol, 12.5% v/v glycerol). Then 2 nM capped RNA substrate was added, followed by incubation for 2 hrs at 30 ° C. The reaction was terminated by incubation at 95 ° C. for 5 min. 
         [0122]    For the detection of the synthesized mRNA the Quantigene® 2.0 (Panomics. 2007. QuantiGene 2.0 Reagent System. User Manual) was used with the probe sets specified. The probe sets consists of Capture Extenders (CE), Label Extenders (LE) and Blocking Probes (BP) and were generated by and supplied as a mix of all three by Affymetrix/Panomics. The probe sequences are represented in SEQ ID NOs: 5 to 20 and are also given in  FIG. 1 . 
         [0123]    The response values (relative luminescence units) were analyzed using GraphPad Prism to determine IC 50  values and 95% confidence intervals using a 4-parameter logistic equation. Positive and negative controls were included to define top and bottom for fitting the curve. 
         [0124]    De novo synthesized viral mRNA was generated by incubating purified RNPs with a capped RNA substrate of known sequence. 
         [0125]    The Quantigene® probe set “TA assay” detects newly synthesized viral mRNA coding for nucleoprotein (NP), the Label Extenders (LE1 and LE2) specifically hybridize to the snatched cap sequence 5′-cap-GGGGGAAUACUCAAG-3′ (SEQ ID NO: 2) cleaved off from the 44-mer RNA substrate and to the polyA sequence, respectively. The Capture Extenders (CE1-9) specifically hybridize to regions within the coding region of the IAV NP gene. Probe set “+RNA” detects positive sense viral RNA coding for NP by specifically binding to more than 10 different regions within the gene. LE and CE of this probe set hybridize to regions between nucleotides 1 and 1540 (GenBank CY147505) and does not distinguish between viral mRNA and viral cRNA. The third probe set “−RNA” specifically hybridized to negative sense RNA (nsRNA), coding for the nonstructural protein (NS). 
         [0126]    Cap Fluorescence-Polarization Ligand Displacement (CapFP-LD) Assay 
         [0127]    The expression construct for PB2 cap binding domain (PB2-CBD) (residues 318-483) of the avian influenza strain A/duck/Shantou/4610/2003(H5N1) was synthesized by Geneart AG. Purified protein was kindly provided by Stephen Cusack and his co-workers (EMBL Grenoble; Guilligay et al., 2008). The PB2-CBD concentration was determined by OD 280  measurement using the extinction coefficient of 6990 M −1 ·cm −1  at 280 nm, m 7 GTP-5FAM (Jena Bioscience) was used as a fluorescent tracer. The concentrations of tracer and receptor were chosen according to their K d  value of 0.42 μM determined in assay buffer (10 mM HEPES pH 7.4, 100 mM NaAc, 10 mM Mg(Ac) 2 , 0.005% (v/v) protein-grade TWEEN 20) (Nikolovska-Coleska et al., 2004). A series of 2-fold dilutions of compound were prepared, transferred to 384-well plates (Corning #3676) at a final DMSO concentration of 10% (v/v). The tracer/protein mixture was added to a final concentration of 2 μM and 1.2 μM respectively. The plates were sealed, incubated and shaken for 30 min before FP was measured. The data was analyzed using GraphPad Prism to determine IC 50  values and 95% confidence intervals using a 4-parameter logistic equation. Positive and negative controls were included to define top and bottom for curve fitting. 
         [0000]    
       
         
               
               
               
             
           
               
                   
               
               
                 Formula 
                 F p   
                 CPE 
               
               
                   
               
             
             
               
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 &gt;50 μmol 
                 &gt;50 μmol 
               
               
                   
               
               
                 (comparative) 
                   
                   
               
               
                   
               
               
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 &gt;50 μmol 
                 &gt;50 μmol 
               
               
                   
               
               
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 K i  = 27.3 μM 
                 &gt;50 μmol 
               
               
                   
               
               
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 K i  = 175.9 μM 
                 IC 50  = 38.5 μM 
               
               
                   
               
               
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 K i  = 9.9 μM 
                 IC 50  = 9.1 μM 
               
               
                   
               
               
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 K i  = 39.7 μM 
                 &gt;50 μmol 
               
               
                   
               
               
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 K i  = 19.8 μM 
                 &gt;50 μmol 
               
               
                   
               
             
          
         
       
     
         [0000]    
       
         
               
               
               
             
           
               
                   
               
               
                 Formula 
                 TA 
                 CPE 
               
               
                   
               
             
             
               
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 TA IC 50  &gt; 33.3 μM 
                 &gt;50 μmol 
               
               
                   
               
               
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 TA IC 50  = 14.3 μM 
                 &gt;50 μmol 
               
               
                   
               
               
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 TA IC 50  &gt; 25 μM 
                 &gt;50 μmol 
               
               
                   
               
               
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 &gt;33.3 
                 &gt;50 μmol 
               
               
                   
               
               
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 44.9 
                 &gt;50 μmol 
               
               
                   
               
               
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 &gt;33.3 
                 IC 50  = 39.3 μM 
               
               
                   
               
               
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 TA IC 50  &gt; 33.3 μM 
                 &gt;50 μmol 
               
               
                   
               
               
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 TA IC 50  &gt; 33.3 μM 
                 &gt;50 μmol 
               
               
                   
               
               
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 &gt;33.3 
                 &gt;50 μmol 
               
               
                   
               
               
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 73.2 
                 &gt;50 μmol 
               
               
                   
               
               
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 148.7 
                 IC 50  &gt; 100 μM 
               
               
                   
               
             
          
         
       
     
         [0128]    In the following, the compounds were prepared according to the general schemes, unless a specific synthesis method is given. 
         [0000]    
       
                 
         
             
             
         
       
     
         [0129]    Synthesis of Compound 6: 
         [0130]    To the solution of cis-3-aminocyclohexanecarboxylic acid (5.0 g, 34.96 mmol) in methanol (120 mL) was added SOCl 2  (8.3 g, 69.93 mmol) at 0 ° C. The mixture was stirred for 5 h at room temperature. Methanol was removed under reduced pressure to afford the crude product (6.5 g) as a white solid, which was used for the next step without further purification. 
         [0131]    Synthesis of Compound 7: 
         [0132]    6 (6.5 g, 33.67 mmol), 2,4-dichloro-5-fluoropyrimidine (6.7 g, 40.12 mmol) and diisopropyl ethyl amine (9.5 g, 73.64 mmol) were added to CH 3 CN (60 mL). This reaction mixture was reflux overnight. The reaction was cooled to room temperature and concentrate in vacuo. The residue was diluted with water and extracted with ethyl acetate. The organic layer was washed with brine, dried over Na 2 SO 4 , filtered and concentrated in vacuo to afford 7 (8.9 g, 91%) as a pale white solid. 
         [0133]    Synthesis of Compound 8: 
         [0134]    To a solution of 7 (8.9 g, 31.01 mmol) in tetrahydrofuran (20 mL) and water (20 mL) was added LiOH*H 2 O (3.9 g, 93.03 mmol). This reaction mixture was stirred overnight at room temperature. After that, the mixture was adjusted to pH=5 with 2N HCl. The resultant was extracted with ethyl acetate. The organic layer was washed with brine, dried over Na 2 SO 4 , filtered and concentrated in vacuo to afford 8 (7.5 g, 89%) as a white solid. 
         [0000]    
       
                 
         
             
             
         
       
     
         [0135]    Synthesis of CAP-004-41-9: 
         [0136]    A solution of 8 (1.00 g, 3.66 mmol), Diphenylphosphoryl azide (1.20 g, 4.40 mmol) and Et 3 N (0.74 g, 7.33 mmol) in toluene (10 mL) was degassed for 2 h. The reaction mixture was heated at 85 ° C. for 40 min. To the reaction mixture was added pyrrolidine (0.52 g, 7.33 mmol) and the reaction was left to room temperature with stirring for 2 h. The mixture was diluted with water and extracted with ethyl acetate. The organic layer was washed with brine, dried over Na 2 SO 4 , filtered and concentrated in vacuo to afford CAP-004-41-9 (0.94 g, 75%) as a pale white solid. 
       N-(3-(2-Chloro-5-fluoropyrimidin-4-ylamino)cyclohexyl)pyrrolidine-1-carboxamide (CAP-004-41-9) 
       [0137]    
       
                 
         
             
             
         
       
     
         [0138]    Pale white solid 
         [0139]    Yield: 1% 
         [0140]    MS (ESI): 342.0 (M+H) +   
         [0141]      1 H NMR (CDCl 3 , 400 MHz): δ=7.88 (d, J=2.8 Hz, 1H), 5.32 (br, 1H), 5.10 (d, J=6.8 Hz, 1H), 4.05-4.13 (m, 1H), 3.82 (t, J=10.8 Hz, 1H), 3.30-3.36 (m, 4H), 2.44 (d, J=10.8 Hz, 1H), 2.08 (d, J=14.0 Hz, 2H), 1.92 (t, J=6.8 Hz, 4H), 1.82-1.88 (m, 1H), 1.46-1.58 (m, 1H), 1.04-1.20 (m, 3H). 
         [0000]    
       
                 
         
             
             
         
       
     
         [0142]    Synthesis of CAP-004-41-1: 
         [0143]    CAP-004-41-9 (0.45 g, 1.32 mmol), 6-chloropyridin-2-ylboronic acid (0.25 g, 1.58 mmol), X-phos (2-Dicyclohexylphosphino-2′, 4′, 6′-triisopropylbiphenyl) (100 mg), Pd 2 (dibenzylideneacetone) 3  (70 mg) and K 2 CO 3  (0.36 g, 2.64 mmol) were added to toluene (2 mL). This reaction mixture was stirred for 2 h at 160 ° C. udner microwave. This mixture was filtered and purified by preparative TLC with ethyl acetate to afford CAP-004-41-1 (46 mg, 8%) as a yellow solid. 
         [0144]    Synthesis of CAP-004-41-2: 
         [0145]    CAP-004-41-1 (40 mg, 0.096 mmol) and hydrazine hydrate (1 mL) were added to EtOH (1 mL). This reaction mixture was stirred for 75 min at 106 ° C. under microwave irradiation. This mixture was concentrated in vacuo. The residue was diluted with water and extracted with ethyl acetate. The organic layer was dried over Na 2 SO 4 , filtered and concentrated in vacuo to afford crude CAP-004-41-2 (32 mg, 82%) as a yellow oil, which was used for the next step without further purification. 
         [0146]    Synthesis of CAP-004-41: 
         [0147]    CAP-004-41-2 (32 mg, 0.076 mmol) and 1,1′-carbonyldiimidazole (20 mg, 0.115 mmol) were added to tetrahydrofuran (5 mL). This reaction mixture was stirred for 40 min at 60 ° C. This mixture was diluted with water and extracted with ethyl acetate. The organic phase was dried over Na 2 SO 4 , filtered and concentrated in vacuo. The residue was purified by preparative HPLC to afford CAP-004-41 (6.4 mg, 18%) as a yellow solid. 
       N-(3-(5-Fluoro-2-(3-oxo-2,3-dihydro-[1,2,4]triazolo[4,3-a]pyridin-5-yl)pyrimidin-4-ylamino)cyclohexyl)pyrrolidine-1-carboxamide (CAP-004-41) 
       [0148]    
       
                 
         
             
             
         
       
     
         [0149]    Yellow solid 
         [0150]    Yield: 1% 
         [0151]    MS (ESI): 441.0 (M+H) +   
         [0152]      1 H NMR (CD 3 OD, 400 MHz): δ=8.93 (s, 1H), 8.34 (s, 1H), 7.61 (s, 1H), 7.40 (d, J=5.2 Hz, 1H), 7.31-7.35 (m, 1H), 7.17 (s, 1H), 4.22-4.29 (m, 1H), 3.65-3.72 (m, 1H), 2.26 (d, J =11.6 Hz, 1H), 2.02 (d, J=12.8 Hz, 1 H), 1.86-1.93 (m, 8H), 1.25-1.50 (m, 6H). 
       N-((1R,3S)-3-(5-Fluoro-2-(3-oxo-2,3-dihydro-[1,2,4]triazolo[4,3-a]pyridin-6-yl)pyrimidin-4-ylamino)cyclohexyl)pyrrolidine-1-carboxamide (CAP-004-42) 
       [0153]    
       
                 
         
             
             
         
       
     
         [0154]    Yellow solid 
         [0155]    Yield: 1% 
         [0156]    MS (ESI): 441.1 (M+H)+ 
         [0157]      1 H NMR (CD 3 OD, 400 MHz): δ=8.72 (s, 1H), 8.25 (d, J=4.4 Hz, 1H), 7.99 (dd, J=10.0 Hz, 1.6 Hz, 1H), 7.34 (dd, J =9.6 Hz, 0.8 Hz, 1H), 4.32-4.40 (m, 1H), 3.74-3.82 (m, 1H), 3.31 (s, 2H), 2.30 (d, J =10.8 Hz, 1H), 2.07 (d, J=12.0 Hz, 1H), 1.87-1.97 (m, 7H), 1.26-1.64 (m, 5H). 
         [0158]    CAP-004 Series: 
         [0000]    
       
                 
         
             
             
         
       
     
         [0159]    Synthesis of CAP-004-D: 
         [0160]    A mixture of CAP-004-06-B (1.00 g, 4.69 mmol), 4,4,4′,4′,5,5,5′,5′-octamethyl-2,2′-bi(1,3,2-dioxaborolane) (1.79 g, 7.05 mmol), potassium acetate (1.38 g, 14.07 mmol) and (1,1′-bis(diphenylphosphino)ferrocene)PdCl 2  (0.19 g, 0.23 mmol) in dioxane (20 mL) was refluxed overnight under N 2  atmosphere. The mixture was cooled to room temperature. The precipitate was removed by filtration and the filtrate was concentrated to give CAP-004-D (3.00 g) as a crude product. 
         [0161]    General Procedures for the Synthesis of CAP-004-X Series: 
         [0162]    A mixture of CAP-004-D (0.60 g (crude product)), RCl (0.90 mmol), K 2 CO 3  (0.37 g, 2.70 mmol) and (1,1′-bis(diphenylphosphino)ferrocene)PdCl 2  (73.44 mg, 0.09 mmol) in dioxane/H 2 O (10 mL/1 mL) was refluxed for 2 h under N 2  atmosphere. The solvent was removed under reduced pressure and the residue was diluted with water (10 mL). The resultant was extracted with ethyl acetate (5 mL×3). The organic phase was washed with brine (5 mL x 3), dried over NaSO 4  and concentrated. The residue was purified by preparative TLC and then purified by preparative HPLC to give CAP-004-X. 
         [0163]    General Procedures for the Synthesis of CAP-004-X-A Series: 
         [0164]    A mixture of CAP-004-X (0.08 mmol) and LiOH*H 2 O (34 mg, 0.80 mmol) in methanol/tetrahydrofuran (2 mL/2 mL) was stirred at room temperature overnight. The solvent was removed under reduced pressure and the residue was dissolved in water (2 mL). The resultant was adjusted to pH=3˜4 with 2N HCl. The mixture was extracted with ethyl acetate (3 mL×5). The organic phase was washed with brine (3 mL×3), dried over NaSO 4  and concentrated to give CAP-004-X-A. 
       Methyl 3-cyclopropyl-3-(5-fluoro-2-(3-oxo-2,3-dihydro-[1,2,4]triazolo[4,3-a] pyridine-6-yl)pyrimidin-4-ylamino)propanoate (CAP-004-45) 
       [0165]    
       
                 
         
             
             
         
       
     
         [0166]    CAP-004-45 was obtained as a yellow solid. 
         [0167]    Yield: 15% 
         [0168]    MS (ESI): 373 (M+H) +   
         [0169]      1 H NMR (CD 3 OD, 400 MHz): δ=8.73 (s, 1H), 8.18 (d, J=4.0 Hz, 1H), 8.06 (d, J=10.0 Hz, 1H), 7.03 (d, J=9.2 Hz, 1H), 4.14-4.19(m, 1H), 3.65 (s, 3H), 2.80-2.92 (m, 2H), 1.19-1.24 (m, 1H), 0.56-0.66 (m, 2H), 0.42-0.48 (m, 2H). 
       3-Cyclopropyl-3-(5-fluoro-2-(3-oxo-2,3-dihydro-[1,2,4]triazolo[4,3-a]pyridin-6-yl)pyrimidin-4-ylamino)propanoic acid (CAP-004-45-A) 
       [0170]    
       
                 
         
             
             
         
       
     
         [0171]    CAP-004-45-A was obtained as a yellow solid. 
         [0172]    Yield: 51% 
         [0173]    MS (ESI): 359 (M+H) +   
         [0174]      1 H NMR (CD 3 OD, 400 MHz): δ=8.73 (s, 1H), 8.14 (d, J=10.0 Hz, 1H), 8.09 (d, J=3.6 Hz, 1H), 7.25 (d, J=10.0 Hz, 1H), 4.15-4.20 (m, 1H), 2.78-2.82 (m, 2H), 1.20-1.31 (m, 1H), 0.60-0.62 (m, 1H), 0.42-0.56 (m, 3H). 
       (R)-Methyl 3-(5-fluoro-2-(3-oxo-2,3-dihydro-[1,2,4]triazolo[4,3-a]pyridine-6-yl) pyrimidin-4-ylamino)-4-methylpentanoate (CAP-004-46) 
       [0175]    
       
                 
         
             
             
         
       
     
         [0176]    CAP-004-46 was obtained as a yellow solid. 
         [0177]    Yield: 13% 
         [0178]    MS (ESI): 375 (M+H) +   
         [0179]      1 H NMR (CD 3 OD, 400 MHz): δ=8.75 (s, 1H), 8.18 (d, J=4.4 Hz, 1H), 8.69 (dd, J=9.6 Hz, 1.6 Hz, 1H), 7.27 (dd, J=10.0 Hz, 1.2 Hz, 1H), 4.75-4.91 (m, 1H), 3.61 (s, 3H), 2.77-2.82 (m, 1H), 2.66-2.70 (m, 1H), 2.01-2.06 (m, 1H), 1.05 (d, J=6.8 Hz, 3H), 1.03 (d, J=6.8 Hz, 3H). 
       (R)-3-(5-Fluoro-2-(3-oxo-2,3-dihydro-[1,2,4]triazolo[4,3-a]pyridin-6-yl)pyrimidin-4-ylamino)-4-methylpentanoic acid (CAP-004-46-A) 
       [0180]    
       
                 
         
             
             
         
       
     
         [0181]    CAP-004-46-A was obtained as a yellow solid. 
         [0182]    Yield: 62% 
         [0183]    MS (ESI): 361 (M+H) +   
         [0184]      1 H NMR (d-DMSO, 400 MHz): δ=12.61 (s, 1H), 8.52 (s, 1H), 8.23 (d, J=3.6Hz, 1H), 7.96 (d, J=8.8 Hz, 1H), 7.76 (d, J=7.6 Hz, 1H), 7.32 (d, J=9.2 Hz, 1H), 4.57-4.59 (m, 1H), 2.59 (s, 2H), 1.91-2.02 (m, 1H), 0.92 (d, J=4.4 Hz, 6H). 
       6-(5-Fluoro-4-(2-hydroxyethylamino)pyrimidin-2-yl)-[1,2,4]triazolo[4,3-a]pyridin-3(2H)-one (CAP-004-47) 
       [0185]    
       
                 
         
             
             
         
       
     
         [0186]    CAP-004-47 was obtained as a white solid. 
         [0187]    Yield: 6% 
         [0188]    MS (ESI): 291 (M+H) +   
         [0189]      1 H NMR (CD 3 OD, 400 MHz): δ=8.76 (s, 1H), 8.16 (d, J=4.0 Hz, 1H), 8.09 (dd, J=10.0 Hz, 1.6 Hz, 1H), 7.28 (d, J=10.0 Hz, 1H), 3.78-3.82 (m, 4H). 
       6-(4-(Cyclohexylamino)-5-fluoropyrimidin-2-yl)-[1,2,4]triazolo[4,3-a]pyridin-3(2H)-one (CAP-004-48) 
       [0190]    
       
                 
         
             
             
         
       
     
         [0191]    CAP-004-48 was obtained as a yellow solid. 
         [0192]    Yield: 7% 
         [0193]    MS (ESI): 329 (M+H) +   
         [0194]      1 H NMR (d-DMSO, 400 MHz): δ=12.60 (s, 1H), 8.49 (s, 1H), 8.20 (d, J=4.0 Hz, 1H), 7.96 (dd, J =11.2 Hz, 1.2 Hz, 1H), 7.67 (d, J=7.2 Hz, 1H), 7.30 (d, J=10.0 Hz, 1H), 4.00 (br, 1H), 1.94 (s, 2H), 1.78 (s, 2H), 1.57 (d, J=13.2 Hz, 1H), 1.32-1.43 (m, 4H), 1.08-1.17 (m, 1H). 
       (S)-Methyl 2-(5-fluoro-2-(3-oxo-2,3-dihydro-[1,2,4]triazolo[4,3-a]pyridine-6-yl) pyrimidin-4-ylamino)-3-methylbutanoate (CAP-004-49) 
       [0195]    
       
                 
         
             
             
         
       
     
         [0196]    CAP-004-49 was obtained as a yellow solid. 
         [0197]    Yield: 9% 
         [0198]    MS (ESI): 361 (M+H) +   
         [0199]      1 H NMR (CD 3 OD, 400 MHz): δ=8.73 (s, 1H), 8.21 (d, J=4.0 Hz, 1H), 8.09 (dd, J=9.6 Hz, 1.2 Hz, 1H), 7.27 (d, J=9.2 Hz, 1H), 4.56 (d, J=7.6 Hz, 1H), 3.81 (s, 3H), 2.30-2.36 (m, 1H), 1.14 (d, J =6.8 Hz, 3H), 1.09 (d, J=6.8 Hz, 3H). 
       (S)-2-(5-Fluoro-2-(3-oxo-2,3-dihydro-[1,2,4]triazolo[4,3-a]pyridin-6-yl)pyrimidin-4-ylamino)-3-methylbutanoic acid (CAP-004-49-A) 
       [0200]    
       
                 
         
             
             
         
       
     
         [0201]    CAP-004-49-A was obtained as a yellow solid. 
         [0202]    Yield: 52% 
         [0203]    MS (ESI): 347 (M+H) +   
         [0204]      1 H NMR (CD 3 OD, 400 MHz): δ=8.75 (s, 1H), 8.18 (d, J=3.6 Hz, 1H), 8.13 (dd, J=9.6 Hz, 1.2 Hz, 1H), 7.26 (d, J=10.0 Hz, 1H), 4.61 (d, J=6.8 Hz, 1H), 2.32-2.40 (m, 1H), 1.11 (t, J=7.2 Hz, 6H). 
       (1R,3S)-Methyl 3-(5-fluoro-2-(3-oxo-2,3-dihydro-[1,2,4]triazolo[4,3-a]pyridin-6-yl)pyrimidin-4-ylamino)cyclohexanecarboxylate (CAP-004-50) 
       [0205]    
       
                 
         
             
             
         
       
     
         [0206]    CAP-004-50 was obtained as a yellow solid. 
         [0207]    Yield: 3% 
         [0208]    MS (ESI): 387 (M+H) +   
         [0209]      1 H NMR (CD 3 OD, 400 MHz): δ=8.73 (s, 1H), 8.08-8.12 (m, 2H), 7.28 (d, J=10.0 Hz, 1H), 4.26-4.32 (m, 1H), 3.69 (s, 3H), 2.62-2.66 (m, 1 H), 2.30-2.33 (m, 1 H), 1.94-2.10 (m, 3H), 1.39-1.62 (m, 4H). 
       (1R,3S)-3-(5-Fluoro-2-(3-oxo-2,3-dihydro-[1,2,4]triazolo[4,3-a]pyridin-6-yl)pyrimidin-4-ylamino)cyclohexanecarboxylic acid (CAP-004-50-A) 
       [0210]    
       
                 
         
             
             
         
       
     
         [0211]    CAP-004-50-A was obtained as a yellow solid. 
         [0212]    Yield: 56% 
         [0213]    MS (ESI): 373 (M+H) +   
         [0214]      1 H NMR (CD 3 OD, 400 MHz): δ=8.73 (s, 1H), 8.09-8.14 (m, 2H), 7.28 (d, J=10.0 Hz, 1H), 4.24-4.32 (m, 1H), 2.55-2.63 (m, 1H), 2.30-2.33 (m, 1H), 1.94-2.11 (m, 3H), 1.43-1.66 (m, 4H). 
       6-(4-(Cyclopropylmethylamino)-5-fluoropyrimidin-2-yl)-[1,2,4]triazolo[4,3-a]pyridin-3(2H)-one (CAP-004-51) 
       [0215]    
       
                 
         
             
             
         
       
     
         [0216]    CAP-004-51 was obtained as a yellow solid. 
         [0217]    Yield: 5% 
         [0218]    MS (ESI): 301 (M+H) +   
         [0219]      1 H NMR (CD 3 OD, 400 MHz): δ=8.75 (s, 1H), 8.16 (d, J=4.0 Hz, 1H), 8.06 (dd, J=10.0 Hz, 1.6 Hz, 1H), 7.30 (d, J=10.0 Hz, 1H), 3.52 (d, J=6.8 Hz, 2H), 1.21-1.27 (m, 1H), 0.59 (dd, J=8.8 Hz, 2.0 Hz, 2H), 0.37 (dd, J=14.0 Hz, 4.4 Hz, 2H). 
       6-(5-Fluoro-4-(methylamino)pyrimidin-2-yl)[1,2,4]triazolo[4,3-a]pyridin-3(2H)-one (CAP-004-52) 
       [0220]    
       
                 
         
             
             
         
       
     
         [0221]    CAP-004-52 was obtained as a white solid. 
         [0222]    Yield: 4% 
         [0223]    MS (ESI): 261 (M+H) +   
         [0224]      1 H NMR (d-DMSO, 400 MHz): δ=12.6 (s, 1H), 8.54 (s, 1H), 8.21 (d, J=3.6 Hz, 1H), 8.08 (dd, J=10.0 Hz, 1.2 Hz, 1H), 7.85 (d, J=2.8 Hz, 1H), 7.30 (d, J=10.0 Hz, 1H), 2.98 (d, J=4.4 Hz, 3H). 
       6-(5-Fluoro-4-(3-methylbutan-2-ylamino)pyrimidin-2-yl)-[1,2,4]triazolo[4,3-a]pyridin-3(2H)-one (CAP-004-53) 
       [0225]    
       
                 
         
             
             
         
       
     
         [0226]    CAP-004-53 was obtained as a yellow solid. 
         [0227]    Yield: 3% 
         [0228]    MS (ESI): 317 (M+H) +   
         [0229]      1 H NMR (CD 3 OD, 400 MHz): δ=8.61 (s, 1H), 8.09 (d, J=4.4 Hz, 1H), 8.86 (dd, J=10.0 Hz, 1.6 Hz, 1H), 7.21 (dd, J=10.0 Hz, 0.8 Hz, 1H), 4.24-4.28 (m, 1H), 1.82-1.87 (m, 1H), 1.19 (d, J=6.4 Hz, 3H), 0.92 (dd, J=6.8 Hz, 3.6 Hz, 6H). 
       6-(5-Fluoro-4-(isopropylamino)pyrimidin-2-yl)[1,2,4]triazolo[4,3-a]pyridin-3(2H)-one (CAP-004-54) 
       [0230]    
       
                 
         
             
             
         
       
     
         [0231]    CAP-004-54 was obtained as a yellow solid. 
         [0232]    Yield: 3% 
         [0233]    MS (ESI): 289 (M+H) +   
         [0234]      1 H NMR (CD 3 OD, 400 MHz): δ=8.62 (s, 1H), 8.06 (d, J=4.4 Hz, 1H), 7.89 (dd, J=10.0 Hz, 1.6 Hz, 1H), 7.19 (dd, J=10.0 Hz, 0.8 Hz, 1H), 4.61-4.53 (m, 1H), 1.25 (d, J=6.4 Hz, 6H). 
       Methyl 6-(5-fluoro-2-(3-oxo-2,3-dihydro-[1,2,4]triazolo[4,3-a]pyridine-6-yl) pyrimidin-4-ylamino)hexanoate (CAP-004-56): SVA-17233 
       [0235]    
       
                 
         
             
             
         
       
     
         [0236]    CAP-004-56 was obtained as a yellow solid. 
         [0237]    Yield: 5% 
         [0238]    MS (ESI): 375 (M+H) +   
         [0239]      1 H NMR (CD 3 OD, 400 MHz): δ=8.74 (s, 1H), 8.15 (d, J =4 Hz, 1H), 8.05 (dd, J=10.0 Hz, 1.6 Hz, 1H), 7.29 (dd, J=10.0 Hz, 0.8 Hz, 1H), 3.64-3.68 (m, 5H), 2.37 (t, J=7.6 Hz, 2H), 1.70-1.77 (m, 4H), 1.45-1.49 (m, 2H). 
       6-(5-Fluoro-2-(3-oxo-2,3-dihydro-[1,2,4]triazolo[4,3-a]pyridin-6-yl)pyrimidin-4-ylamino)hexanoic acid (CAP-004-56-A) 
       [0240]    
       
                 
         
             
             
         
       
     
         [0241]    CAP-004-56-A was obtained as a white solid. 
         [0242]    Yield: 56% 
         [0243]    MS (ESI): 361 (M+H) +   
         [0244]      1 H NMR (d6-DMSO, 400 MHz): δ=12.58 (s, 1H), 12.00 (s, 1H), 8.51 (s, 1H), 8.20 (d, J=3.6 Hz, 1H), 7.97 (dd, J=10.0 Hz, 1.6 Hz, 1H), 7.87 (s, 1H), 7.29 (d, J=10.0 Hz, 1H), 3.45-3.50 (m, 2H), 2.22 (t, J=7.6 Hz 2H), 1.54-1.65 (m, 4H), 1.34-1.38(m, 2H).