Abstract:
In the invention, a fluid cell, used for sequencing reaction between DNA fragments and reagents, comprises: a reaction chamber, one inner side of which is fixed with multiple DNA fragments; a reagent inlet and a reagent outlet, which are separately located at each end of the other inner side of the reaction chamber and used for reagents flowing into and out from the reaction chamber. Multiple DNA fragments are fixed on the reaction chamber with the small capacity as short tag arrays in sequence and make the reaction go on at the condition of the reagents without diffusion barriers, which improves the contacts of reagents and DNA fragments so as to shorten the reaction time. At the same time, the demands to the concentration and dosage of reagents are low, which reduces the consumption of reagents. As the result, the sequencing cost cut down. Multiple DNA fragments are fixed on the fluid cell at the same time, which provides a way for parallel reaction of various fragments. The automatic high-through sequencing and fast sequencing reactions are achieved in the invention.

Description:
FIELD OF THE INVENTION 
       [0001]    The present invention relates to the flied of nucleotide, and more particularly to a fluid cell a gene sequencing reaction platform and a gene sequencing system. 
       BACKGROUND OF THE INVENTION 
       [0002]    Traditional Sequencing technology distinguishes the difference of DNA fragment lengths using the method of gel electrophoresis with stepwise paused polymerization process. 
         [0003]    One of the present technologies mixes in the dideoxyribonucleoside triphosphate (ddNTP) during DNA polymerization, which lacks a hydroxyl at the 3′ position of the nucleotide, so it can not form Phosphodiester bond with the following dNTP. In the presence of ddCTP, dCTP and three other dNTP, mixing in Primers, Templates and DNA polymerase together and maintaining the temperature, the polymerization process will form a mixture of multiple fragments with different length but containing the same 5′-end primers and ddC residue on the 3′end. The various population of products in each dideoxyribonucleoside triphosphate (ddNTP) group (each has one of the four different bases labeled) are separated according to different lengths of chains, and the corresponding radioactive autoradiography or fluorescence spectra is obtained. Sequences of DNA bases can be read from the spectra. Using this technology for single gene fragment sequencing, the read length of sequences can be long. Since gene fragments must be handled individually when adding samples, the data throughput can not be improved greatly, the number is still between dozen to dozens. Moreover, it needs to separately clone each fragment, which is complicated and time-consuming. 
         [0004]    Therefore a new technology is much needed to improve the efficiency of gene sequencing. 
       BRIEF SUMMARY OF THE INVENTION 
       [0005]    The present invention provides a fluid cell for rapid high-throughput sequencing. 
         [0006]    In order to achieve the goal of the present invention, a fluid cell is used to carry out the reaction between DNA fragments and reagents. The present invention comprises: a reaction chamber, one inner side of which is fixed with multiple DNA fragments; a reagent inlet and a reagent outlet, which are separately located at each end of the other inner side of the reaction chamber and used for reagents flowing into and out from the reaction chamber. 
         [0007]    Wherein, the fluid cell comprises: a drilled cover slide, a slide which is parallel and opposite to the drilled cover slide, and a gasket between the drilled cover slide and the slide; the gasket has through-holes in its central section, and is pressed closely with the drilled cover slide and the slide on its two sides separately; the through-holes, inner side of the drilled cover slide and inner side of the slide form the closed reaction chamber; as one side of the reaction chamber, the inner side of the slide is fixed with DNA fragments; as the other side of the reaction chamber, two ends of the drilled cover slide have the first and second through-hole, which are used for connecting the reaction chamber with outside separately, and form the said reagent inlet and reagent outlet. 
         [0008]    Wherein: the through-hole of the gasket is narrow on both ends, and wide in middle, which looks like a leaf; said both ends of the through-hole separately correspond to the first through-hole and the second through-hole of the drilled cover slide, forming a reagent inlet and a reagent outlet. 
         [0009]    Wherein: in the reaction chamber, the one side and/or the other side which fixed with DNA fragments are transparent. 
         [0010]    Wherein: DNA fragments are attached to at least one bead which is fixed on the inner side of reaction chamber. 
         [0011]    Wherein: DNA fragments are directly fixed on one inner side of the reaction chamber. 
         [0012]    In order to achieve the invention&#39;s aims better, the present invention also provides a gene sequencing reaction platform for controlling sequencing reaction between the DNA fragments and reagents, which platform comprises: a fluid cell, the inner side of which is fixed with multiple DNA fragments; a temperature control unit, which is used to heat and control the temperature of sequencing reaction; a reagent control unit, which is used to control the reagents of sequencing reaction. 
         [0013]    Wherein, the temperature control unit comprises a heating component and a temperature-measuring component: the temperature-measuring component measures temperature of sequencing reaction in real-time mode, and the measured temperature is used to control the heating component to heat the fluid cell; the heating component heats the fluid cell on the side which is fixed with DNA fragments, or on the other side. 
         [0014]    Wherein, further comprising heating glasses which are used to heat the sequencing reaction; said heating glasses cover the outside of the fluid cell, which is heated by the heating component, and then heat and control temperature of the sequencing reaction in the reaction chamber through heat conduction of the fluid cell. 
         [0015]    wherein the reagent control unit comprises: a reagent device which stores at least one kind of reagent; a reagent valve used for selecting one kind or several kinds of stored reagents from the reagent device; a reagent-injection component, which injects selected reagents into the fluid cell; a reagent-ejection component, used for ejecting reacted reagents from the fluid cell. 
         [0016]    Wherein: the said fluid cell is located at level, upright or oblique. 
         [0017]    In order to achieve the purpose of the invention better, the invention also provides a gene sequencing system for controlling the sequencing reaction between DNA fragments and reagents, and collecting and processing the data of sequencing reaction, which system comprises: a fluid cell, fixed with multiple DNA fragments and used for the gene sequencing reaction; an imaging unit, used for imaging the signal on the said sequencing DNA fragments; a data-collecting unit, used for collecting the image data; control units, used for controlling the sequencing reaction and processing image data. 
         [0018]    Wherein the fluid cell comprises: a reaction chamber, in which multiple DNA fragments are fixed on one inner side; a reagent inlet and a reagent outlet, which are separately located at each end of the other inner side of the reaction chamber and used for reagents flowing into and out from the reaction chamber. 
         [0019]    Wherein: the DNA fragments attached to at least one bead which is fixed on one inner side of the fluid cell; or the DNA fragments are directly fixed on the inner side of the fluid cell. 
         [0020]    Wherein the control units comprise: a temperature control unit, used to heat and control temperature of the sequencing reaction; a reagent control unit, used to control the reagents, comprising selecting, injecting and/or ejecting; a data-processing unit used to send the control orders to the temperature unit and the reagent control unit and manage, analyze and process the image data. 
         [0021]    Wherein the temperature control unit further comprises a heating component and a temperature-measuring component: the temperature-measuring component measures temperature of sequencing reaction in real-time mode and sends the measured temperature to the data-processing unit to analyze the measured temperature and generate temperature controlling orders; the heating component heats the fluid cell according to the temperature controlling orders from the data-processing unit. 
         [0022]    Wherein the imaging unit comprises: a light source, which is fixed on one side of the fluid cell; an imaging component and a compound lens, which are located on the same side of the fluid cell; the imaging component takes pictures of the fluid cell through the compound lens. 
         [0023]    Wherein: the fluid cell is located at level, upright or oblique. 
         [0024]    Wherein the gene sequencing system further comprises: a supporting unit, used for anchoring and/or arranging the fluid cell and the imaging unit separately, in order to make the gene sequencing system stable. 
         [0025]    Wherein the supporting unit further comprises: a position adjusting device, used for changing the distance between the fluid cell and the imaging component. 
         [0026]    In the present invention, lots of DNA fragments fixed on small volume reaction chamber as short tags arrays in sequencing, which makes sequencing reaction processes at the condition of reagents without diffusion barrier, resulting in improving the contact between reagents and DNA fragments and shortening the time of reaction. The demands on concentration and dose of reagent are low, that is to say the consumption of reagent is less and the cost of sequencing is lower. With multiple DNA fragments fixed on a reaction chamber at the same time, it provides a platform for parallel reaction of lots of DNA fragments. This invention makes high-through sequencing automatic come into truth, integrates reaction temperature controlling, reagent controlling, imaging, data collection and procession and so on. It achieves quick sequencing reaction. Moreover, the much more through of sequence is read and efficiency is high. 
     
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         [0027]      FIG. 1  is the structure diagram of the fluid cell in the first embodiment of the present invention. 
           [0028]      FIG. 2  is the detail structure diagram of the fluid cell in the second and the seventh embodiments of the present invention. 
           [0029]      FIG. 3  is the exploded structure diagram of the reaction chamber in the second embodiment of the present invention. 
           [0030]      FIG. 4  is the structure diagram of the components of gene sequencing reaction platform in the third embodiment of the present invention. 
           [0031]      FIG. 5  is the detail structure diagram of the gene sequencing reaction platform in the third embodiment of the present invention. 
           [0032]      FIG. 6  is another detail structure diagram of gene sequencing reaction platform in the fourth embodiment of the present invention. 
           [0033]      FIG. 7  is the structure diagram of the components of gene sequencing system in the fifth embodiment of the present invention. 
           [0034]      FIG. 8  is the structure diagram of the control units of the fifth embodiment in  FIG. 7 . 
           [0035]      FIG. 9  is the detail components structure diagram of the gene sequencing system in the sixth embodiment. 
           [0036]      FIG. 10  is the part components structure diagram of the gene sequencing system in the seventh and the eighth embodiments. 
       
    
    
       [0037]    According to the embodiments and the figures to further explain the achievements of goal, features and advantages of the present invention. 
       DETAILED DESCRIPTION OF THE INVENTION 
       [0038]    By way of fixing DNA fragments in fluid cell to form short tags array in sequencing in the present invention, reagents directly flow through DNA fragments, therefore avoiding diffusion barrier and achieving the sequencing reaction of reagent and DNA fragments. 
         [0039]    The first embodiment is proved in the invention. According to the design scheme of fluid cell in  FIG. 1 , the fluid cell  1  comprises the reaction chamber  11 , the reagent inlet  12  and the reagent outlet  13 . The DNA fragments  100  are fixed on the one side of reaction chamber  11  inner side. The reagent inlet  12  and the reagent outlet  13  are separately set at the other side of the two ends of reaction chamber  11 . The reagent inlet  12  is used for reagents flowing into reaction chamber  11 . The reagent outlet  13  is used for reagents flowing out from reaction chamber  11 . 
         [0040]    The one side and/or the other side of reaction chamber  11  for fixing DNA fragments  100  are transparent. 
         [0041]    The fluid cell  1  is used for gene Sequencing and the DNA fragments  100  are fixed in reaction chamber  11  for reagents going through the reagent inlet  12  to reaction chamber  11  and reacting sequencing reaction with the DNA fragments  100 . The DNA fragments  100  which have already reacted are observed and/or imaged from the one side of fixed DNA fragments  100  in reaction chamber  11 . The reagents after the sequencing reaction flow out from reaction chamber  11  through reagent outlet  13 . 
         [0042]    The DNA fragments  100  can be separately fixed on one inner side of reaction chamber  11  in the embodiment and also attached to at least one bead, which is fixed on the inner side of reaction chamber  11 . 
         [0043]    After DNA fragments  100  is fixed on one inner side of reaction chamber  11 , a cover made by soft material overlaps the surface. There is at least one array made by series of small holes on the cover, for the purpose of forming independent reaction chambers, where the DNA molecules amplify separately and form addressable DNA fragments  100  array on the surface. This surface fixed with DNA fragments  100  is the right one inner side of reaction chamber  11 . The DNA fragments  100  are attached to at least one bead which is fixed on the inner side of reaction chamber  11 , which fixes the DNA fragments  100  on the discrete solid surfaces by the method marking up biotin at the end of DNA chain. For example, the DNA fragments  100  are fixed to isolated sites or individual beads on the surface. The isolated sites or individual beads are fixed on the inner sides of reaction chamber  11  in order to form DNA array of at least one sites. 
         [0044]    Based on the first embodiment, the invention proves the second embodiment and takes DNA fragments fixed on beads for example.  FIG. 2  shows the detail structure diagram of fluid cell  1 . The fluid cell  1  comprises: a drilled cover slide  14 , the slide  15 , the two of which is parallel and opposite, and the gasket  16  lies between the drilled cover slide  14  and the slide  15 . According to the  FIG. 3  about exploded structure diagram of the reaction chamber  11 , there is a through-hole  161  in the center of the gasket  16 . The both sides of gasket  16  closely paste the drilled cover slide  14  and the slide  15 . The through-hole  161 , the inner side of the drilled cover slide  14  and the slide  15  form the closed reaction chamber  11 . As one inner side of reaction chamber  11 , the inner side of the slide  15  has lots of beads  10  on which fixed with the DNA fragments  100  (Not shown in figure). As the other inner side of reaction chamber  11 , the two ends of the drilled cover slide  14  has the first through-hole  141  and the second through-hole  142 , which make the reaction chamber  11  conduct with outside, forming reagent inlet  12  and reagent outlet  13 . 
         [0045]    In this embodiment, according to pre-set, beads  10 , at least one site fixed on the inner side of the slide  15 , form the enriched beads arrays or plane lattices. It should be noticed that the beads  10  can be make by many kinds of materials, such as glass, plastic, metal and so on. 
         [0046]    Drilled cover slide  14  chooses smooth slides or quartz plates, using the technology of laser or mechanical drilling to form the first through-hole  141  and the second through-hole  142 . The slide  15  uses the smooth slides. The drilled cover slide  14  and/or the slide  15  are transparent. The gasket  16 , the tow sides of which attach with the drilled cover slide  14  and the slide  15 , using the self-adhesive gasket and softness macromolecule material is preferred, such as Silicone, rubber, PDMA, PS and so on. The through-hole  161  of gasket  16  shows the leaf shape with the two narrow ends and the wide middle. The two narrow ends of through-hole  161  is corresponding with the first through hole  141  and the second through-hole  142  of the drilled cover slide  14  and form the reagent inlet  12  and reagent outlet  13 . The gasket  16  has a large area connecting with the drilled cover slide  14  and the slide  15 , forming a hermetic space at the condition of non-pressure. 
         [0047]    The fluid cell  1  can get different capacity and size by changing the thickness of the gasket  16  and the area of the through-hole  161  in this embodiment. The thickness of the gasket  16  can be chose according to demands and the optional range is from 0.125 mm to 0.5 mm. The area of the through-hole  161  can be also chose according to demands and 240 mm 2  is a preferential choice. The optional capacity of formed reaction chamber  11  is 60 ml. 
         [0048]    The optional area of thought-hole  161  is 240 mm 2  in the embodiment, which is enough to accommodate more than 5 million beads  10  with 5 um diameter, or 16 million plastic beads with 3 um diameter, or 1.2 billion beads with 1 um diameter at the fill rate of 50%. The thickness of 250 um to the gasket  16  and the area of 240 mm 2  to the through-hole  161  is the optional value, which forms the optional accommodation of 60 ml to reaction chamber  11 . Expensive reagents (such as Ligase and Fluorescent oligonucleotides, Polymerase and Fluorescent nucleotide) can be maximized to use in the embodiment. 
         [0049]    The capacity of reaction chamber  11  can be chose in order to meet practical requirements in the embodiment. To change the capacity of reaction chamber  11  is just need to change the size of the gasket  16 . Usually, pre-experiments of sequencing uses only small amount of reagent to make sure the feasibility of experiments, so it is favorable to do pre-experiments of sequencing. Because the capacity of the reaction chamber  11  is small, without diffusion barrier and the speed of sequencing reaction is fast, the reagent consumption of sequencing reaction is small and less than 10% of the present technology solutions. As a result, the cost of sequencing is further reduced. 
         [0050]    In order to be easier to inject the reagents, in the embodiment the inlet pipe  17  and the outlet pipe  18 , separately connecting with the reagent inlet  12  and the reagent outlet  13 , is installed, which makes the reagents flow into the reaction chamber  11  and the reagents flow out from the reaction chamber  11  successfully. 
         [0051]    Using the fluid cell  1  to gene sequencing has the similar principle of working with the previous embodiment. The detail is that many beads  10  are fixed on the inner side of the slide  15 . The reagent goes along the inlet pipe  17 , and goes through the reagent outlet  13 , and flows into the reaction chamber  11 , then comes into contact with the DNA fragments  100  on beads  10 , thus starts the sequencing reaction. DNA fragments  100  of occurring sequencing reaction are observed and/or image through the slide  15  and/or bleed cover slide  14 . The reagent, after sequencing reaction, goes through the reagent outlet  13  and passes the outlet pipe  18  and then flows out from reaction chamber  11 . 
         [0052]    The third embodiment is proved in the invention.  FIG. 4  shows a gene sequencing reaction platform which comprises the fluid cell  1 , the temperature control unit  2  using to heat and control the temperature for sequencing reaction and a reagent control unit  3  to control the reagent of sequencing reaction. 
         [0053]    The temperature control unit  2  comprises: the heating component  21  and the temperature-measuring component  22 . The temperature-measuring component  22  measures the temperature of sequencing reaction in real-time mode. The measured temperature is used for controlling the heating component  21  to heat the fluid cell  1 . 
         [0054]    In order to control the sequencing temperature, based on the previous embodiments using a heating element  19  to heat sequencing reaction is proved in the embodiment.  FIG. 5  shows the detail structure diagram of gene sequencing reaction platform. The heating element  19  which covers the outside of fluid cell  1  is heated by heating component  21 , which makes the sequencing reaction in reaction chamber  11  be heated and controls the reaction temperature through the heat conduction of fluid cell  1 . The option to the heating element  19  is tin indium oxide (ITO) coated glass. 
         [0055]    The reagent control unit  3  comprises: a reagent device  31 , a reagent valve  32 , a reagent-injection component  33  and a reagent-ejection component  34 . The reagent device  31  stores at least one kind of reagent. The reagent valve  32  selects one kind of reagent or more from the various reagents. The component  33  injects the selected reagent to the reaction chamber  11  of fluid cell  1  through inlet pipe  17 . The reagent-ejection component  34  ejects the reagent reacted sequencing reaction from the fluid cell  1  going through the outlet pipe  18 . 
         [0056]    The reagent injection component  33  and/or the reagent-ejection component  34  choose the syringe liquid pumps in the embodiment. 
         [0057]    Preferably, in the embodiment the fluid cell  1  comprises: the drilled cover slide  14 , the slide  15 , the two of which is parallel and opposite, and the gasket  16  located between the drilled cover slide  14  and the slide  15 . In  FIG. 5 , the center of the gasket  16  has the through-hole  161 . The two sides of gasket  16  tightly glue the drilled cover slide  14  and the slide  15 . The through-hole  161 , the inner side of the drilled cover slide  14  and the inner side of the slide  15  form the closed reaction chamber  11 . One inner side of the slide  15  fixed on multiple DNA fragments  100  (Not shown in figure) or the beads attached multiple DNA fragments (following called beads  10  for short) as to be one inner side of reaction chamber  11 . In the embodiment, the heating element  19  attaches to the outside of the drilled cover slide  14  of fluid cell  1 . The heating element  19  which is heated by the heating component  21  heats for the sequencing reaction of reaction chamber  11  by the heat-conduction of the drilled cover slide  14  and controls the reaction temperature. This is to say that the heating element  19  heats the side of fluid cell  1  which does not fix DNA fragments  100  or beads  10 . 
         [0058]    The heating element  19  is made by the tin indium oxide (ITO) coated glass. The drilled cover slide  14  is made by slide or quartz plate to achieve the good heat conduction. 
         [0059]    Using the gene sequencing reaction platform for gene sequencing, the detail is that multiple DNA fragments  100  or beads  10  are fixed in the fluid cell  1 . The reagent valve  32  selects the reagent from the reagent device  31  and the reagent-injection component  33  injects reagents to fluid cell  1  through the inlet pipe  17 . According to temperature measured by the temperature-measuring component  22 , the heating component  21  heats the heating element  19  to control the temperature of sequencing reaction. The reagent in reaction chamber  11  reacts sequencing reaction with DNA fragments  100  and the DNA fragments of reacted sequencing reaction is observed and/or imaged from fluid cell  1 . The reagent-ejection component  34  ejects the reagent reacted the sequencing reaction to the fluid cell  1  by the outlet pipe  18 . 
         [0060]    The heating element  19  and the drilled cover slide  14  have some degree of stability and good heat conduction in the embodiment, which can be used several times in sequencing reaction and make the cost of sequencing reaction further reduced. The heating element  19  and the drilled cover slide  14  have the good transmission of light so it is beneficial to observer and/or image the DNA fragments reacted. Using the embodiment to react the sequencing reaction, an ordinary power of the mercury short-arc lamp can be used as the light source and an ordinary imaging device can be use to observe and/or image, which makes the sequencing cost greatly reduced. 
         [0061]    Based on the above embodiments, the fourth embodiment is provided in the invention. The reagent and the temperature to control sequencing reaction are posed so as to collect and process the image data. 
         [0062]    A gene sequencing reaction platform comprises: the fluid cell  1 , the temperature control unit  2  to heat for sequencing reaction and control temperature, and the reagent control unit  3  to select, inject and/or eject the reagent of sequencing reaction according to the described control units. 
         [0063]    In the  FIG. 6 , compared with the third embodiment, the heating element  19 , in this embodiment, tightly glues on the outside of slide  15  of the fluid cell  1 . The heating element  19  which is heated by heating component  21  heats for the sequencing reaction reacting in the reaction chamber  11  and controls the reaction temperature, which is achieved by the heat conduction of slide  15 . This is to say that the heating element  19  heats the side of fluid cell  1  fixed with multiple DNA fragments  100  (not shown in the figure) or beads  10 . The option to the heating element (heating element)  19  is tin indium oxide (ITO) coated glass. The clear glass or quartz plate is used for slide  15  so as to achieve good heat conduction. 
         [0064]    The heating element  19  and slide  15  have some degree of stability and good heat conduction in the embodiment, which can be used multiple times in sequencing reaction and make the cost of sequencing reaction further reduced. The heating element  19  and the slide  15  have good transmission of light so it is beneficial to observer and/or image the DNA fragments reacted. Using the embodiment to carry out the sequencing reaction, an ordinary power of the mercury short-arc lamp can be used as the light source and an general imaging device can be used to observe and/or image, which makes the sequencing cost greatly reduced. 
         [0065]    The embodiment further comprises a pedestal  7  for fixing the fluid cell  1  and keeping its stability so that the sequencing reaction goes smoothly. 
         [0066]    The fluid cell  1  in this embodiment and the above embodiment can be located at level, upright or oblique. For example, the fluid cell  1  in the second embodiment has the drilled cover slide  14  and the slide  15 , the two of which is parallel and opposite, which can be located at level, upright or certain angle so as to make the reagent inlet  12  and reagent outlet  13  at the same level. In order to improve the result of imaging, the fluid cell  1  is located at upright in this embodiment. Reagents inject from one end of reaction chamber  11  and eject from the other end. The described one end can be higher than the other and also can be lower. 
         [0067]    Preferably, the reagents inject from the lower end of reaction chamber  11  and eject from the higher end, which fully fills the reaction chamber  11  and contacts with DNA fragments  100  to make sure the DNA fragments  100  and the reagents fully react in the sequencing reaction. As a result, the quality of image data is much higher. 
         [0068]    The fifth embodiment is posed in the invention. The  FIG. 7  shows the gene sequencing system which comprises: the fluid cell  1  fixed with multiple DNA fragments and used to react gene sequencing reaction, the imaging unit  4  used to observe and/or image the sequencing reaction, the data-collecting unit  5  used to collect the image data and the control units  6  used to control the temperature and/or reagents of sequencing reaction and process the collected data. The imaging unit  4  collects the image data from the sequencing reaction in the fluid cell  1 . The data-collecting unit  5  sends the gained image data to the control units  6 . And then, the control units  6  manage, analyze and process the image data. 
         [0069]      FIG. 8  shows the structure of control units  6  which comprise: a temperature control unit  2 , a reagent control unit  3  and data-processing unit  61 . The temperature control unit  2  heats for the sequencing reaction and controls the temperature. The reagent control unit  3  controls the reagent of sequencing reaction, comprises selecting, injecting and/or ejecting. The data-processing unit  61  manages, analyzes and processes the image data, and then sends the control orders to the temperature control unit  2  and the reagent control unit  3 . 
         [0070]    Based on the above embodiments, the sixth embodiment is posed in the invention. The  FIG. 9  shows the gene sequencing system. In this embodiment, the temperature control unit  2  comprises: the heating component  21  and the temperature-measuring component  22 . The temperature-measuring component  22  measures the temperature of sequencing reaction and then sends it to the data-processing unit  61 . The data-processing unit  61  analyzes measured temperature, and generates temperature controlling orders, and then sends out the temperature controlling orders. The orders are sent to the heating component  21  which heats for the fluid cell  1  and uses the tin indium oxide (ITO) coated glass to heat. 
         [0071]    In the embodiment, the reagent control unit  3  comprises: a reagent device  31 , a reagent valve  32 , a reagent-injection component  33  and a reagent-ejection component  34 . The reagent device  31  stores various reagents. The data-processing unit  61  controls the reagent valve  32  to select one kind of reagent or more from the various reagents and then controls the reagent-injection component  33  to injected into the reaction chamber  11  through the inlet pipe  17 . The data-processing unit  61  controls the reagent-ejection component  34  to eject the reacted reagent from the fluid cell  1  through the outlet pipe  18 . 
         [0072]    In the embodiment, the imaging unit  4  comprises: a light source  41 , a compound lens  42  and an imaging component  43  (Not shown in  FIG. 9 ). The light source  41  is fixed on one side of the fluid cell  1 . The compound lens  42  and the imaging component  43  locate at the same side on the fluid cell  1 . The lens components  42  and the imaging component  43  are used to image for the fluid cell  1 . The light source  41  uses the mercury lamp light source. The compound lens  42  comprises: an incident light filter, a spectroscope, focus imaging lens assembly and emitted light filter. The imaging devices  43  uses a CCD probe. 
         [0073]    In the embodiment, the signal intensity of sequencing reaction is strong. As a result, not only the ordinary mercury short-arc lamp can be used as the light source  41  to light for the fluid cell  1 , but also the imaging component  43  can use the high specification CCD probe, such as the CCD probe from 4 million to 11 million pixels. The data-collecting unit  5  uses a reader (Sub-second time for full size), which can achieve high speed data collecting and improve the data throughput of the whole data. In the embodiment, the size and capacity of the fluid cell  1  can be selected flexibly to fit for the actual demands. The small size of fluid cell  1  makes the pixel of the whole imaging device  43  be used. To gain enough image data for gene sequencing, it is only needs 0.5-5 seconds to get a single image. 
         [0074]    In the embodiment, the light source  41  and a compound lens  42  form the focus imaging system through a spectral-filter, irradiating on the DNA fragments  100  in the fluid cell  1 . which also image multicolor image by auto-switching the optical filter model. 
         [0075]    In the embodiment, the image data is analyzed and a database can also be created to manage the image data. Specifically, using the database to store and manage the image data gained from at least one site. Extract the data file about the signal strength after calculating the generating reaction. 
         [0076]    In the embodiment, the data-processing unit  61  of the gene sequencing system controls the cycle sequencing of DNA fragments, which integrates the various functions about temperature controlling, reagent controlling, imaging, data collecting and processing. 
         [0077]    Based on the above embodiments, the seventh embodiment is posed. According to the  FIG. 2 , the fluid cell  1  of gene sequencing system comprises: the drilled cover slide  14 , the slide  15 , the two of which is parallel opposite, and the gasket  16  located between the drilled cover slide  14  and the slide  15 . The center of the gasket  16  has the through-hole  161 . The two sides of the gasket  16  are tightly adhered to the drilled cover slide  14  and the slide  15 . The through-hole  161 , the inner side of the drilled cover slide  14  and the inner side of the slide  15  form a closed reaction chamber  11 . As one inner side of reaction chamber  11 , the inner side of the slide  15  fixes multiple DNA fragments  100  or beads  10 . 
         [0078]    According to  FIG. 10 , the light source  41  is fixed on one side of the drilled cover slide  14 . The imaging component  43  and the compound lens  42  are located at the same side of the fluid cell  1 . Through the compound lens  42  and crossing the slide  15 , the imaging component  43  images the sequencing reaction of reaction chamber  11 . 
         [0079]    The light source  41  also can be fixed on one side of the slide  15  on fluid cell  1 . The imaging component  43  and the compound lens  42  are located on one side of the drilled cover slide  14  on the fluid cell  1 . Through the compound lens  42  and the drilled cover slide  14 , the imaging component  43  images the sequencing reaction of reaction chamber  11 . Compared with the style through the slide  15  to image the sequencing reaction of reaction chamber  11 , using this style makes the sequencing reaction more fully and the imaging result better, but the structure of sequencing reaction is more complex. 
         [0080]    Based on the above embodiments, the eighth embodiment is posed. The  FIG. 10  shows some parts of structure of gene sequencing system. The gene sequencing system further comprises a supporting unit  8  which fixes or arrays the various units, including the fluid cell  1  of the gene sequencing system, the temperature control unit  2 , the reagent control unit  3  (Not shown in the figure), imaging unit  4  and so on, and make the units meet the position and working relationship. At the same time, it makes the gene sequencing system stable. 
         [0081]    Specifically, the supporting unit  8  comprises: a backplane  81  of the system, a platform  82  set on the system backplane  81  and the at least two mainstays  83  to sure the platform  82  stability and reducing the shaking. The supporting unit  8  further comprises the first mainstay  84  and the second mainstay  85  which are plumb with the platform  82 . the first mainstay  84  and the second mainstay  85 , the two of which is parallel and exist side by side, are used for anchoring and supporting the fluid cell  1 , the temperature control unit  2 , the reagent control unit  3  (Not shown in the figure), the imaging unit  4  and so on. In order to operate the gene sequencing system automatically, it also can install a position adjusting equipment in the first mainstay  84  and the second mainstay  85  to adjust the relationship of position about the fluid cell  1 , the temperature control unit  2 , the reagent control unit  3  (Not shown in figure), the imaging unit  4  and so on. The position adjusting equipment can be made up by a knob with a screw. 
         [0082]    In the embodiment, the process of the reaction is: the various different reagents of sequencing reaction are sub-packaged in the reagent device  31  by the reagent valve  32 . When the reaction is going on, the control units  6  select the demanded reagents and control the reagent-injection component  33  to take quantitative reagent and inject the fluid cell  1 . The temperature control unit  2  adjusts and controls the temperature of the fluid cell  1  to make sure the reaction go smoothly. After finished every reaction, the control units  6  select the washing reagents from the reagent device  31  to clean the fluid cell  1  and then change the reagents the next step need. After finished the whole sequencing reaction, the bases&#39; information of DNA fragments is recorded by the imaging unit  4 . Adjusting the position adjusting equipment to make the DNA fragments fixed at different position aim at the imaging unit  4  so as to collect the image data of the DNA fragments about each imaging area in the fluid cell  1  for storing and later analyzing. 
         [0083]    In the embodiment and above embodiments, the fluid cell  1  can be located at level, upright or oblique. For example, in the second embodiments, the drilled cover slide  14  and the slide  15  which is parallel and opposite to the fluid cell  1  can be located at level, upright or certain angle, which makes the reagent inlet  12  and the reagent outlet  13  at the different level. 
         [0084]    In order to avoid the bubble affecting imaging and ensure the imaging effect, the fluid cell  1  is set upright in the embodiments. The reagents inject into the reaction chamber  11  from the button and eject from the top. 
         [0085]    The fluid cell  1  can also be mounted at the tilted position. The two ends are different in height. Reagents inject into reaction chamber  11  from the lower end and eject from the higher end, which makes the reagents full upon the reaction chamber  11  and DNA fragment  100  fully contact so as to ensure the DNA fragments  100  react thoroughly with the reagents and avoid air bubbles that may affect imaging. As a result, the quality of image data is better. 
         [0086]    The above mentioned is only the optional embodiment of the invention, but doesn&#39;t limit the scope of patent about the present invention. Any using the content of specification and/or drawing of the invention to change the equal structure and process directly or indirectly, applying to the related technology fields, all above conditions are equal to contain in the scope of patent protection.