Abstract:
Adenoviral serotypes differ in their natural tropism. The various serotypes of adenovirus have been found to differ in at least their capsid proteins (e.g., penton-base and hexon proteins), proteins responsible for cell binding (e.g, fiber proteins), and proteins involved in adenovirus replication. This difference in tropism and capsid proteins among serotypes has led to the many research efforts aimed at redirecting the adenovirus tropism by modification of the capsid proteins. The present invention bypasses such requirement for capsid protein modification as it presents a recombinant, replication-defective adenovirus of serotype 24, a rare adenoviral serotype, and methods for generating the alternative, recombinant adenovirus. Additionally, means of employing the recombinant adenovirus for the delivery and expression of exogenous genes are provided.

Description:
CROSS-REFERENCE TO RELATED APPLICATIONS  
       [0001]    This application claims the benefits of U.S. provisional application serial No. 60/455,312, filed Mar. 17, 2003 
     
    
     
       BACKGROUND OF THE INVENTION  
         [0002]    Adenoviruses are nonenveloped, icosahedral viruses that have been identified in several avian and mammalian hosts; Home et al., 1959  J Mol. Biol . 1:84-86; Horwitz, 1990 In  Virology , eds. B. N. Fields and D. M. Knipe, pps. 1679-1721. The first human adenoviruses (Ads) were isolated over four decades ago. Since then, over 100 distinct adenoviral serotypes have been isolated which infect various mammalian species, 51 of which are of human origin; Straus, 1984, In  The Adenoviruses , ed. H. Ginsberg, pps. 451-498, New York:Plenus Press; Hierholzer et al., 1988  J. Infect. Dis . 158:804-813; Schnurr and Dondero, 1993 , Intervirology ;36:79-83; Jong et al., 1999  J Clin Microbiol ., 37:3940-5. The human serotypes have been categorized into six subgenera (A-F) based on a number of biological, chemical, immunological and structural criteria which include hemagglutination properties of rat and rhesus monkey erythrocytes, DNA homology, restriction enzyme cleavage patterns, percentage G+C content and oncogenicity; Straus, supra; Horwitz, supra.  
           [0003]    The adenovirus genome is very well characterized. It consists of a linear double-stranded DNA molecule of approximately 36,000 base pairs, and despite the existence of several distinct serotypes, there is some general conservation in the overall organization of the adenoviral genome with specific functions being similarly positioned.  
           [0004]    Adenovirus has been a very attractive target for delivery of exogenous genes. The biology of adenoviruses is very well understood. Adenovirus has not been found to be associated with severe human pathology in immuno-competent individuals. The virus is extremely efficient in introducing its DNA into the host cell and is able to infect a wide variety of cells. Furthermore, the virus can be produced at high virus titers in large quantities. In addition, the virus can be rendered replication defective by deletion of the essential early-region  1  (E1) of the viral genome; Brody et al, 1994  Ann N.Y. Acad Sci ., 716:90-101.  
           [0005]    Replication-defective adenovirus vectors have been used extensively as gene transfer vectors for vaccine and gene therapy purposes. These vectors are propagated in cell lines that provide E1 gene products in trans. Supplementation of the essential E1 gene products in trans is very effective when the vectors are from the same or a very similar serotype. E1-deleted group C serotypes (Ad1, Ad2, Ad5 and Ad6), for instance, grow well in 293 or PER.C6 cells which contain and express the Ad5 E1 region. However, the Ad5 E1 sequences in 293 or PER.C6 cells do not fully complement the replication of all serotypes other than group C. This is perhaps due to the inability of the Ad5 (group C) E1b 55K gene product to functionally interact with the E4 gene product(s) of the non-group C serotypes. Although the interaction is conserved within members of the same subgroup, it has not been found to be well conserved between subgroups. In order to successfully and efficiently rescue recombinant adenovirus of alternative, non-group C serotypes, a cell line expressing the E1 region of the serotype of interest would have to be generated. Alternatively, available Ad5E1-expressing cell lines could be modified to express Ad5E4 (or Orf6) in addition to Ad5E1. These additional, sometimes tedious and daunting tasks, impeded the production of recombinant, non-group C adenoviral vectors.  
           [0006]    An efficient means for the propagation and rescue of alternative serotypes in an Ad5 E1-expressing cell line (such as PER.C6 or 293) was disclosed in pending U.S. provisional application (U.S. Ser. No. 60/405,182, filed Aug. 22, 2002). This method involves the incorporation of a critical E4 region into the adenovirus to be propagated. The critical E4 region is native to a virus of the same or highly similar serotype as that of the E1 gene product(s), particularly the E1B 55K region, of the complementing cell line, and comprises, in the least, nucleic acid encoding E4 Orf6.  
           [0007]    Presently, two well-characterized adenovirus serotypes from subgroup C, Ad5 and Ad2, are the most widely used gene delivery vectors. There is a need to develop alternate Ad serotypes as gene transfer vectors since neutralizing antibodies in the general population may limit primary dosing or redosing with the same serotype. The prevalence of neutralizing antibody can vary from serotype to serotype. Neutralizing antibodies to some serotypes such as Ad5 are common, while antibodies to others are relatively rare. Alternate serotypes, furthermore, possess alternate tropisms which may lead to the elicitation of superior immune responses when used for vaccine or gene therapy purposes.  
           [0008]    Adenovirus serotype 24, a subgroup D adenovirus, was originally isolated in 1960 (S. D. Bell et al., 1960  Am. J Trop. Hyg . 9:523) and established as a recognized reference strain in 1963 (H. G. Pereira et al., 1963  Virology  20:613). Its antigenic relationship to 46 other human adenoviruses determined in reference horse antisera has been discussed; J. C. Hierholzer et al., 1991  Arch. Virol . 121:179-197. The partial sequence of Ad24 hexon (1091 of 2838 bp) was disclosed in Takeuchi et al., 1999  J. Clin. Microbiol . 37:3392-3394, and deposited in Gen Bank (accession no. AB023553). The sequence of the virus associated RNA (VA RNA) for Ad24 and partial sequence for the pre-terminal protein and 52/55K proteins (521 bp) was disclosed in Ma &amp; Matthews, 1993  J. Virol . 67:6605-6617, and Gen Bank (accession No. HAU52544).  
           [0009]    The fields of vaccines and gene therapy would greatly benefit from additional knowledge concerning alternative adenoviral serotypes, particularly those serotypes such as Ad24 which are not well represented in the human population. Of particular interest are recombinant adenoviral vectors based on alternative adenoviral serotypes, and means of obtaining such recombinant adenoviral vectors. This need in the art is met with the disclosure of the present application related to recombinant adenoviral vectors based on adenoviral serotype 24.  
         SUMMARY OF THE INVENTION  
         [0010]    The present invention relates to recombinant, replication-deficient adenovirus vectors of serotype 24, a rare adenoviral serotype, and methods for generating the recombinant adenovirus based on the alternative serotype. Additionally, means of employing the recombinant adenovirus for the delivery and expression of exogenous genes are provided. The invention, thus, encompasses recombinant, replication-defective adenoviral vectors of serotype 24 which comprise one or more transgenes operatively linked to regulatory sequences which promote effective expression of the respective transgene(s). Host administration of such recombinant adenovirus serotype 24 vectors, whether administered alone or in a combined modality and/or prime boost regimen, results in the efficient expression of the incorporated transgene and effectively induces an immune response capable of specifically recognizing the particular antigen administered (e.g., HIV). Furthermore, the recombinant virus should evade pre-existing immunity to adenovirus serotypes which are more commonly encountered in the human population (e.g., Ad5 and Ad2). The disclosed methods, thus, present an enhanced means for inducing an immune response against a particular antigen of interest (e.g., HIV). Accordingly, the resultant immune response should offer a prophylactic advantage to previously uninfected individuals and/or provide a therapeutic effect by reducing viral load levels within an infected individual, thus prolonging the asymptomatic phase of infection.  
       
    
    
     BRIEF DESCRIPTION OF THE DRAWINGS  
       [0011]    [0011]FIG. 1 illustrates the homologous recombination scheme utilized to recover pAd24ΔE1.  
         [0012]    [0012]FIG. 2 illustrates the homologous recombination scheme utilized to recover pAd24ΔE1Ad5Orf6.  
         [0013]    [0013]FIG. 3 illustrates the configuration of E4 regions in the Ad24 recombinants generated.  
         [0014]    [0014]FIG. 4 illustrates the growth kinetics of the Ad24-based vectors in PER.C6 cells.  
         [0015]    [0015]FIGS. 5A-1 through  5 A- 10  illustrate the nucleic acid sequence for wild-type adenovirus serotype 24 (SEQ ID NO: 1). The ATCC product number for Ad24 is VR-259.  
         [0016]    [0016]FIG. 6 illustrates, in tabular format, gag-specific T cell responses in monkeys immunized with MRKAd5-HIVgag and Ad24 HIV vectors. Shown are the numbers of spot-forming cells per million PBMC following incubation in the absence (mock) or presence of Gag peptide pool. The pool consisted of 20-aa peptide overlapping by 10 aa and encompassing the entire gag sequence.  
         [0017]    [0017]FIG. 7 illustrates, in tabular format, the characterization of the gag-specific T cells in monkeys immunized with 10{circumflex over ( )}11 vp of MRKAd5-HIV1gag and Ad24ΔE1gagΔOrf6Ad5Orf6. Shown are the percentages of CD3+ T cells that are either gag-specific CD4+ or gag-specific CD8+ cells. These values were corrected for mock values (&lt;0.03%).  
         [0018]    [0018]FIG. 8 illustrates individual anti-p24 titers (in mMU/mL) in macaques immunized with gag-expressing adenovirus vectors.  
         [0019]    [0019]FIG. 9 illustrates in vivo expression of SEAP in C3H/HeN mice using 10{circumflex over ( )}10 vp doses of Ad24 vectors. The vectors were injected intramuscularly and the levels of SEAP expression were determined from the serum samples. Two extra cohorts received 10{circumflex over ( )}10 vp and 10{circumflex over ( )}9 vp of Ad5 vector. Shown are geometric means for each cohort of 5 mice.  
         [0020]    [0020]FIG. 10 illustrates in vivo SEAP expression using MRKAd5 and Ad24 vectors in rhesus macaques. Shown are the geometric means of the SEAP levels for cohorts of 3 monkeys. In bars are the standard errors of the geometric means.  
         [0021]    [0021]FIG. 11 illustrates a homologous recombination scheme to be utilized to recover pAd24ΔE1ΔE4Ad5Orf6.  
         [0022]    [0022]FIG. 12 illustrates the nucleic acid sequence (SEQ ID NO: 3) of the optimized human HIV-1 gag open reading frame.  
         [0023]    [0023]FIG. 13 illustrates the nucleic acid sequence encoding the gag expression cassette (SEQ ID NO: 4). The various regions of the figure are as follows: (1) a first underlined segment of nucleic acid sequence encoding the immediate early gene promoter region from human cytomegalovirus; (2) a first segment of lowercase letters which is not underlined, which segment of DNA contains a convenient restriction enzyme site; (3) a region in caps which contains the coding sequence of HIV-1 gag; (4) a second segment of lowercase letters which is not underlined, which segment of DNA contains a convenient restriction enzyme site; and (5) a second underlined segment, this segment containing nucleic acid sequence encoding a bovine growth hormone polyadenylation signal sequence.  
         [0024]    [0024]FIG. 14 illustrates the nucleic acid sequence encoding the SEAP expression cassette (SEQ ID NO: 5). The various regions of the figure are as follows: (1) a first underlined segment of nucleic acid sequence encoding the immediate early gene promoter region from human cytomegalovirus; (2) a first segment of lowercase letters which is not underlined, which segment of DNA contains a convenient restriction enzyme site; (3) a region in caps which contains the coding sequence of the human placental SEAP gene; (4) a second segment of lowercase letters which is not underlined, which segment of DNA contains a convenient restriction enzyme site; and (5) a second underlined segment, this segment containing nucleic acid sequence encoding a bovine growth hormone polyadenylation signal sequence.  
         [0025]    [0025]FIGS. 15A-1 through  15 A- 10  illustrate the nucleic acid sequence for wild-type adenovirus serotype 17 (SEQ ID NO: 6; Accession No. AF108105).  
         [0026]    [0026]FIGS. 16A-1 through  16 A- 47  illustrate the nucleotide sequence of the pMRKAd5HIV-1gag vector (SEQ ID NO:7 [coding] and SEQ ID NO:8 [non-coding]).  
         [0027]    [0027]FIGS. 17A-1 through  17 -A- 14  illustrate the nucleic acid sequence for the Ad6 genome (SEQ ID NO: 9).  
         [0028]    [0028]FIG. 18 illustrates gag-specific T cell responses in rhesus macaques immunized following a heterologous Ad5/Ad6 prime-Ad24 boost regimen. a: Mock, no peptide: gag, 20-mer peptide pool encompassing entire gag sequence; b: Peak response after 2 or 3 doses of the priming vaccine; c: 3 wks prior to boost; d: 4 wks after boost; e: ND, not determined.  
         [0029]    [0029]FIG. 19 illustrates, in tabular format, the percentages of CD3 +  T lymphocytes that are gag-specific CD8 +  cells or gag-specific CD4 +  cells determined after the Ad24 Boost Immunization (wk 60). Numbers reflect the percentages of circulating CD3+ lymphocytes that are either gag-specific CD4+ or gag-specific CD8+ cells. Mock values (equal to or less than 0.01%) have been subtracted.  
         [0030]    [0030]FIG. 20 illustrates gag-specific T cell responses in rhesus macaques immunized following a heterologous Ad 24 prime-Ad5 boost regimen. a: Mock, no peptide: gag, 20-mer peptide pool encompassing entire gag sequence; b: Peak response after 2 doses of the priming vaccine; c: Wk 24; d: 4 wks after boost; e: ND, not determined. 
     
    
     DETAILED DESCRIPTION OF THE INVENTION  
       [0031]    Rare adenoviral serotypes possess an inherent advantage over the more commonly exploited adenoviral serotypes (for instance, adenoviral serotypes 2 and 5) since preexisting immunity is unlikely to limit their efficient delivery and expression of exogenous genes to their target site. Different adenoviral serotypes also exhibit distinct tropisms by reason of their varying capsid structure and, thus, present the potential for targeting different tissues and possibly leading to the elicitation of superior immune responses when used for vaccine or gene therapy purposes. These rare adenoviral serotypes when rendered replication-defective, however, can be difficult to propagate and rescue in currently available adenoviral propagation cell lines.  
         [0032]    Applicants have recently managed to successfully rescue and propagate one such rare, replication-defective alternative serotype, adenovirus serotype 24, a subgroup D adenovirus, and herein demonstrate the effective functioning of the adenovirus in the delivery and expression of exogenous transgenes.  
         [0033]    Accordingly, the present invention relates to a recombinant adenoviral vector of serotype 24 suitable for use in gene therapy or vaccination protocols. The nucleic acid sequence for wild-type adenovirus serotype 24 (SEQ ID NO: 1) is illustrated in FIGS. 5A-5J, although any functional homologue or different strain of adenovirus serotype 24 can be utilized in accordance with the methods of the present invention, as one of ordinary skill in the art will appreciate. Adenovirus serotypes have been distinguished through a number of art-appreciated biological, chemical, immunological and structural criteria which include hemagglutination properties of rat and rhesus monkey erythrocytes, DNA homology, restriction enzyme cleavage patterns, percentage G+C content and oncogenicity; Straus, supra; Horwitz, supra. A given serotype can be identified by a number of methods including restriction mapping of viral DNA; analyzing the mobility of viral DNA; analyzing the mobility of virion polypeptides on SDS-polyacrylamide gels following electrophoresis; comparison of squence information to known sequence particularly from capsid genes (e.g., hexon) which contain sequences that define a serotype; and comparing a sequence with reference sera for a particular serotype available from the ATCC. Classification of adenovirus serotypes by SDS-PAGE has been discussed in Wadell et al., 1980  Ann. N.Y. Acad. Sci . 354:16-42. Classification of adenovirus serotypes by restriction mapping has been discussed in Wadell et al.,  Current Topics in Microbiology and Immunology  110:191-220. Reference sera for Ad24 are available from the ATCC (ATCC Product Nos. VR-1102AS/RB and VR-1102PI/RB). Adenovirus serotype 24, a subgroup D adenovirus, was originally isolated in 1960 (S. D. Bell et al., 1960  Am. J. Trop. Hyg . 9:523) and has been established as a recognized reference strain in 1963 (H.G. Pereira et al., 1963 Virology 20:613). Its antigenic relationship to 46 other human adenoviruses determined in reference horse antisera has been discussed in the art; J. C. Hierholzer et al., 1991  Arch. Virol . 121:179-197.  
         [0034]    Adenovirus serotype 24 vectors in accordance with the present invention are at least partially deleted in E1 and devoid (or essentially devoid) of E1 activity, rendering the vector incapable of replication in the intended host. Preferably, the E1 region is completely deleted or inactivated. The adenoviruses may contain additional deletions in E3, and other early regions, albeit in situations where E2 and/or E4 is deleted, E2 and/or E4 complementing cell lines may be required to generate recombinant, replication-defective adenoviral vectors.  
         [0035]    Adenoviral vectors of use in the methods of the present invention can be constructed using well known techniques, such as those reviewed in Hitt et al., 1997 “Human Adenovirus Vectors for Gene Transfer into Mammalian Cells”  Advances in Pharmacology  40:137-206, which is hereby incorporated by reference. Often, a plasmid or shuttle vector containing the heterologous nucleic acid of interest is generated which comprises sequence homologous to the specific adenovirus of interest. The shuttle vector and viral DNA or second plasmid containing the cloned viral DNA are then co-transfected into a host cell where homologous recombination occurs and results in the incorporation of the heterologous nucleic acid into the viral nucleic acid. Preferred shuttle vectors and cloned viral genomes contain adenoviral and plasmid portions. For shuttle vectors used in the construction of replication-defective vectors, the adenoviral portion typically contains non-functional or deleted E1 and E3 regions and the gene expression cassette, flanked by convenient restriction sites. The plasmid portion of the shuttle vector typically contains an antibiotic resistance marker under the transcriptional control of a prokaryotic promoter. Ampicillin resistance genes, neomycin resistance genes and other pharmaceutically acceptable antibiotic resistance markers may be used. To aid in the high level production of the nucleic acid by fermentation in prokaryotic organisms, it is advantageous for the shuttle vector to contain a prokaryotic origin of replication and be of high copy number. A number of commercially available prokaryotic cloning vectors provide these benefits. Non-essential DNA sequences are, preferably removed. It is also preferable that the vectors not be able to replicate in eukaryotic cells. This minimizes the risk of integration of nucleic acid vaccine sequences into the recipients&#39; genome. Tissue-specific promoters or enhancers may be used whenever it is desirable to limit expression of the nucleic acid to a particular tissue type.  
         [0036]    Homologous recombination of the shuttle vector and wild-type adenovirus 24 viral DNA (Ad24 backbone vector) results in the generation of adenoviral pre-plasmids (see, for instance, pAd24ΔE1, pAd24ΔE1Ad5Orf6, pAd24ΔE1gagΔE4Ad5Orf6, pAd24ΔE1 gagΔOrf6Ad5Orf6, pAd24ΔE1SEAPΔE4Ad5Orf6, pAd24ΔE1SEAPΔOrf6Ad5Orf6). Upon linearization, the pre-plasmids are capable of replication in PER.C6® cells or alternative E1-complementing cell lines. Infected cells and media can then be harvested once viral replication is complete.  
         [0037]    A packaging cell will generally be needed in order to produce sufficient amount of adenovirus. The packaging cell should contain elements which are necessary for the production of the specific adenovirus of interest. It is preferable that the packaging cell and the vector not contain overlapping elements which could lead to replication competent virus by recombination. Specific examples of cells which are suitable for the propagation of recombinant Ad24 E1-deleted vectors express the early region 1 (E1) of adenovirus 24 or another group D serotype. Alternatively, propagation cell lines can be used which express adenoviral E1 and E4 regions (particularly, E4 open reading frame 6 (“ORF6”)) which are derived from the same serotype but different subgroup than Ad24 (e.g., Ad5 E1 and E4); see, e.g., Abrahamsen et al., 1997  J Virol . 8946-8951, and U.S. Pat. No. 5,849,561. Additionally, a cell line could be used that expresses E1B from Ad24 in addition to (1) E1A or (2) E1A and E1B from a serotype of a different subgroup. In copending U.S. provisional application serial No. 60/405,182, filed Aug. 22, 2002, a strategy was disclosed for the efficient propagation and rescue of alternative adenoviral serotypes. The method is based on incorporating, into the genome of the adenovirus vector, an E4 region (or portion thereof including E4 ORF6) of the same or highly similar serotype as that of the E1 gene product(s), particularly E1B, being expressed by the complementing cell line. Examples 1-4 demonstrate the viability of such a method through the incorporation of an Ad5E4 region and its propagation in PER.C6 cells (which cells express Ad5E1). The wildtype adenovirus serotype 5 sequence is known and described in the art; see Chroboczek et al., 1992  J Virol . 186:280, which is hereby incorporated by reference. Placement of the E4 region or ORF6-containing portion is not critical; see Examples 1-4. The critical step is making sure that either a promoter is supplied or the gene is strategically placed so that it runs off a promoter native to the vector (e.g., such as the E4 promoter). The native E4 region of the vector can be replaced, deleted or left intact. This method is, thus, suitable for use in the propagation and rescue of the adenoviral vectors of the present invention.  
         [0038]    Typically, propagation cells are human cells derived from the retina or kidney, although any cell line capable of expressing the appropriate E1 and/or E4 region(s) can be utilized in the present invention. Embryonal cells such as amniocytes have been shown to be particularly suited for the generation of E1 complementing cell lines. Several cell lines are available. These include but are not limited to the known cell lines PER.C6 (ECACC deposit number 96022940), 911, 293, and E1 A549.  
         [0039]    The present invention encompasses methods for producing a recombinant, replication-defective adenovirus of serotype 24 in an adenoviral E1-complementing cell line, comprising transfecting a recombinant, replication-defective adenoviral vector of serotype 24 in an adenoviral E1-complementing cell and allowing for the production of viral particles. The viral particles so produced form another aspect of the present invention. Host cells comprising the recombinant, replication-defective adenoviral serotype 24 vectors of the present invention form yet another aspect of the present invention; host cells being defined as a population of cells not including a transgenic human being. Recombinant, replication-defective adenovirus harvested in accordance with the methods of the present invention are encompassed herein as well. This harvested material may be purified, formulated and stored prior to host administration.  
         [0040]    Adenoviral vectors in accordance with the present invention are very well suited to effectuate expression of desired proteins, especially in situations where an individual&#39;s immune response effectively prevents administration or readministration via the more commonly employed adenoviral serotypes. Accordingly, specific embodiments of the present invention are recombinant, replication-defective adenoviral vectors of serotype 24 which comprise a heterologous nucleic acid of interest. The nucleic acid of interest can be a gene, or a functional part of a gene. The nucleic acid can be DNA and/or RNA, can be double or single stranded, and can exist in the form of an expression cassette. The nucleic acid can be inserted in an E1 parallel (transcribed 5′ to 3′) or anti-parallel (transcribed in a 3′ to 5′ direction relative to the vector backbone) orientation. The nucleic acid can be codon-optimized for expression in the desired host (e.g., a mammalian host). The heterologous nucleic acid can be in the form of an expression cassette. A gene expression cassette will typically contain (a) nucleic acid encoding a protein or antigen of interest; (b) a heterologous promoter operatively linked to the nucleic acid encoding the protein; and (c) a transcription termination signal.  
         [0041]    In specific embodiments, the heterologous promoter is recognized by an eukaryotic RNA polymerase. One example of a promoter suitable for use in the present invention is the immediate early human cytomegalovirus promoter (Chapman et al., 1991  Nucl. Acids Res . 19:3979-3986). Further examples of promoters that can be used in the present invention are the strong immunoglobulin promoter, the EF 1 alpha promoter, the murine CMV promoter, the Rous Sarcoma Virus promoter, the SV40 early/late promoters and the beta actin promoter, albeit those of skill in the art can appreciate that any promoter capable of effecting expression in the intended host can be used in accordance with the methods of the present invention. The promoter may comprise a regulatable sequence such as the Tet operator sequence. Sequences such as these that offer the potential for regulation of transcription and expression are useful in instances where repression of gene transcription is sought. The adenoviral gene expression cassette may comprise a transcription termination sequence; specific embodiments of which are the bovine growth hormone termination/polyadenylation signal (bGHpA) or the short synthetic polyA signal (SPA) of 50 nucleotides in length defined as follows: AATAAAAGATCTTTATTTTCATTAGATCTGTGTGTT-GGTTTTTTGTGTG (SEQ ID NO:2). A leader or signal peptide may also be incorporated into the transgene. In specific embodiments, the leader is derived from the tissue-specific plasminogen activator protein, tPA.  
         [0042]    Heterologous nucleic acids of interest are genes (or their functional counterparts) which encode immunogenic and/or therapeutic proteins. Preferred therapeutic proteins are those which elicit some measurable therapeutic benefit in the individual host upon administration. Preferred immunogenic proteins are any proteins which are capable of eliciting an immune response in an individual. Applicants have exemplified the delivery of a representative immunogenic protein (HIV gag) in the present specification in non-human primates (rhesus macaques), albeit any gene encoding a therapeutic or immunogenic protein can be used in accordance with the methods disclosed herein. The adenovirus serotype 24 vectors were found to induce significant levels of gag-specific T cells; FIG. 6. Moreover, the results indicated that immunization with the disclosed vectors was able to elicit both HIV-specific CD4+ and CD8+ T cells; FIG. 7. Additionally, detectable levels of circulating anti-gag antibodies were generated in response to administration of the vector at a dose of 10{circumflex over ( )}11 vp; FIG. 8.  
         [0043]    An aspect of the present invention, therefore, relates to adenovirus serotype 24-based vectors carrying an HIV transgene. In these embodiments, nucleic acid encoding any HIV antigen may be utilized (specific examples of which include gag, pol, nef, gp160, gp41, gp120, tat, and rev, including derivatives of the aforementioned genes). The embodiments exemplified herein employ nucleic acid encoding a codon-optimized p55 gag antigen; see FIG. 12 (SEQ ID NO: 3). Codon-optimized HIV-1 env genes are disclosed in PCT International Applications PCT/US97/02294 and PCT/US97/10517, published Aug. 28, 1997 (WO 97/31115) and Dec. 24, 1997, respectively. Codon-optimized HIV-1 pol genes are disclosed in U.S. application Ser. No. 09/745,221, filed Dec. 21, 2000 and PCT International Application PCTIUS00/34724, also filed Dec. 21, 2000. Codon-optimized HIV-1 nef genes are disclosed in U.S. application Ser. No. 09/738,782, filed Dec. 15, 2000 and PCT International Application PCT/US00/34162, also filed Dec. 15, 2000.  
         [0044]    In this specific embodiment of a recombinant, replication-defective Ad24 vector comprising an HIV-1 gene, the gene may be derived from HIV-1 strain CAM-1; Myers et al, eds. “Human Retroviruses and AIDS: 1995, IIA3-IIA19, which is hereby incorporated by reference. This gene closely resembles the consensus amino acid sequence for the clade B (North American/European) sequence. HIV gene sequence(s) may be based on various clades of HIV-1; specific examples of which are Clades B and C. Sequences for genes of many HIV strains are publicly available from GenBank and primary, field isolates of HIV are available from the National Institute of Allergy and Infectious Diseases (NIAID) which has contracted with Quality Biological (Gaithersburg, Md.) to make these strains available. Strains are also available from the World Health Organization (WHO), Geneva Switzerland. It is well within the purview of the skilled artisan to choose an appropriate nucleotide sequence which encodes a specific HIV antigen, or immunologically relevant portion or modification thereof. “Immunologically relevant” as defined herein means (1) with regard to a viral antigen, that the protein is capable, upon administration, of eliciting a measurable immune response within an individual sufficient to retard the propagation and/or spread of the virus and/or to reduce the viral load present within the individual; or (2) with regards to a nucleotide sequence, that the sequence is capable of encoding for a protein capable of the above.  
         [0045]    The present invention encompasses methods for (1) effectuating a therapeutic response in an individual and (2) generating an immune response (including a cellular-mediated immune response) comprising administering to an individual a recombinant adenovirus serotype 24 vector in accordance with the present invention. One aspect of the present invention are methods for generating an enhanced immune response against one or more antigens (bacterial, viral (e.g., HIV), or other (e.g., cancer)) which comprise the administration of a recombinant adenovirus serotype 24 vehicle expressing the antigen of interest. Administration of recombinant Ad24 vectors in this manner provides for improved cellular-mediated immune responses, particularly where there is pre-existing immunity in a given host to the more well-represented adenovirus serotypes (e.g., Ad2 and Ad5). An effect of the improved vaccine administration methods should be a lower transmission rate to (or occurrence rate in) previously uninfected individuals (i.e., prophylactic applications) and/or a reduction in the levels of virus/bacteria/foreign agent within an infected individual (i.e., therapeutic applications). As relates to HIV indications, an effect of the improved vaccine administration methods should be a lower transmission rate to previously uninfected individuals (i.e., prophylactic applications) and/or a reduction in the levels of viral loads within an infected individual (i.e., therapeutic applications) so as to prolong the asymptomatic phase of HIV infection. Administration, intracellular delivery and expression of the recombinant Ad24 vectors elicits a host CTL and Th response.  
         [0046]    Accordingly, the present invention relates to methodology regarding administration of the recombinant Ad24 viral vectors (or immunogenic compositions thereof, herein termed vaccines) to provide effective immunoprophylaxis, to prevent establishment of an infection following exposure to the viral (for instance, HIV), bacterial or other agent, or as a post -infection therapeutic vaccine to mitigate infection to result in the establishment of a lower virus/bacteria/other load with beneficial long term consequences.  
         [0047]    The recombinant adenovirus serotype 24 vectors of the present invention may be administered alone, or as part of a prime/boost administration regimen. A priming dose(s) of at least one antigen (e.g., an HIV antigen) is first delivered with a recombinant adenoviral vector. This dose effectively primes the immune response so that, upon subsequent identification of the antigen(s) in the circulating immune system, the immune response is capable of immediately recognizing and responding to the antigen(s) within the host. The priming dose(s) is then followed with a boosting dose comprising a recombinant adenoviral vector containing at least one gene encoding the antigen. A mixed modality prime and boost inoculation scheme will result in an enhanced immune response, particularly where there is pre-existing anti-vector immunity. Prime-boost administrations typically involve priming the subject (by viral vector, plasmid, protein, etc.) at least one time, allowing a predetermined length of time to pass, and then boosting (by viral vector, plasmid, protein, etc.). Multiple primings, typically 1-4, are usually employed, although more may be used. The length of time between priming and boost may typically vary from about four months to a year, albeit other time frames may be used as one of ordinary skill in the art will appreciate.  
         [0048]    In addition to a single protein or antigen of interest being delivered by the recombinant, replication-defective adenovirus serotype 24 vectors of the present invention, two or more proteins or antigens can be delivered either via separate vehicles or delivered via the same vehicle. Multiple genes/functional equivalents may be ligated into a proper shuttle plasmid for generation of a pre-adenoviral plasmid comprising multiple open reading frames. Open reading frames for the multiple genes/functional equivalents can be operatively linked to distinct promoters and transcription termination sequences. In other embodiments, the open reading frames may be operatively linked to a single promoter, with the open reading frames operatively linked by an internal ribosome entry sequence (IRES; as disclosed in WO 95/24485), or suitable alternative allowing for transcription of the multiple open reading frames to run off of a single promoter. In certain embodiments, the open reading frames may be fused together by stepwise PCR or suitable alternative methodology for fusing together two open reading frames. Due consideration must be given, however, to the effective packaging limitations of the viral vehicle. Adenovirus, for instance, has been shown to exhibit an upper cloning capacity limit of approximately 105% of the wildtype Ad5 sequence.  
         [0049]    Prime-boost regimens can employ different adenoviral serotypes. One example of such a protocol would be a priming dose(s) comprising a recombinant adenoviral vector of a first sero type followed by a boosting dose comprising a recombinant adenoviral vector of a second and different serotype; see, for instance, Example 11 and FIGS. 18, 19 and  20 . Therein, a cohort of 4 macaques was given three doses of either Ad5- or Ad6-based HIV gag carrying vectors at weeks 0, 4, and 26. At week 56, a booster shot an Ad24-based vector in accordance with the present invention carrying HIV gag was delivered. Administration of the Ad24-based vector resulted in about a 13- to 47-fold enhancement in T cell responses when compared to the levels at the time of booster. In an alternative embodiment, the priming dose can comprise a mixture of separate adenoviral vehicles each comprising a gene encoding for a different protein/antigen. In such a case, the boosting dose would also comprise a mixture of vectors each comprising a gene encoding for a separate protein/antigen, provided that the boosting dose(s) administers recombinant viral vectors comprising genetic material encoding for the same or similar set of antigens that were delivered in the priming dose(s). These multiple gene/vector administration modalities can further be combined. It is further within the scope of the present invention to embark on combined modality regimes which include multiple but distinct components from a specific antigen.  
         [0050]    Compositions, including vaccine compositions, comprising the adenoviral vectors of the present invention are an important aspect of the present invention. These compositions can be administered to mammalian hosts, preferably human hosts, in either a prophylactic or therapeutic setting. Potential hosts/vaccinees include but are not limited to primates and especially humans and non-human primates, and include any non-human mammal of commercial or domestic veterinary importance. Compositions comprising recombinant adenoviral serotype 24 vectors may be administered alone or in combination with other viral- or non-viral-based DNA/protein vaccines. They also may be administered as part of a broader treatment regimen. The present invention encompasses those situations as well where the disclosed recombinant adenoviral serotype 24 vectors are administered in conjunction with other therapies; for example, HAART therapy (in the case of a recombinant HIV vector).  
         [0051]    Compositions comprising the recombinant viral vectors may contain physiologically acceptable components, such as buffer, normal saline or phosphate buffered saline, sucrose, other salts and polysorbate. In certain embodiments, the formulation has: 2.5-10 mM TRIS buffer, preferably about 5 mM TRIS buffer; 25-100 mM NaCl, preferably about 75 mM NaCl; 2.5-10% sucrose, preferably about 5% sucrose; 0.01-2 mM MgCl 2 ; and 0.001%-0.01% polysorbate 80 (plant derived). The pH should range from about 7.0-9.0, preferably about 8.0. One skilled in the art will appreciate that other conventional vaccine excipients may also be used in the formulation. In specific embodiments, the formulation contains 5 mM TRIS, 75 mM NaCl, 5% sucrose, 1 mM MgCl 2 , 0.005% polysorbate 80 at pH 8.0. This has a pH and divalent cation composition which is near the optimum for Ad5 and Ad6 stability and minimizes the potential for adsorption of virus to a glass surface. It does not cause tissue irritation upon intramuscular injection. It is preferably frozen until use.  
         [0052]    The amount of viral particles in the vaccine composition to be introduced into a vaccine recipient will depend on the strength of the transcriptional and translational promoters used and on the immunogenicity of the expressed gene product. In general, an immunologically or prophylactically effective dose of 1×10 7  to 10×10 12  particles and preferably about 1×10 10  to 1×10 11  particles is administered directly into muscle tissue. Subcutaneous injection, intradermal introduction, impression through the skin, and other modes of administration such as intraperitoneal, intravenous, or inhalation delivery are also contemplated. Parenteral administration, such as intravenous, intramuscular, subcutaneous or other means of administration of interleukin-12 protein, concurrently with or subsequent to parenteral introduction of the vaccine compositions of this invention is also advantageous.  
         [0053]    The following non-limiting Examples are presented to better illustrate the workings of the invention.  
       EXAMPLE 1  
       [0054]    Construction and Rescue of pAd24ΔE1.  
         [0055]    An E1- Ad24-based pre-adenovirus plasmid was constructed in order to determine whether an E1- Ad24 vector (a representative group D serotype) could be propagated in an Ad5/group C E1-complementing cell line. Since at the time the vector construction was initiated the complete sequence of Ad24 was unknown we took advantage of some sequence homology between Ad24 and Adl7. The general strategy used to recover Ad24 as a bacterial plasmid is illustrated in FIG. 1 and described below. Cotransformation of BJ5183 bacteria with purified wild-type Ad24 viral DNA and a second DNA fragment termed the Ad17 ITR cassette resulted in the circularization of the viral genome by homologous recombination. The ITR cassette contains sequences from the right (bp 34469 to 35098) and left (bp 4 to 414 and bp 3373 to 4580) end of the Ad17 genome (see FIGS. 15A-15J) separated by plasmid sequences containing a bacterial origin of replication and an Ampicillin resistance gene. The ITR cassette contains a deletion of E1 sequences from Ad17 (bp 415 to 3372) with a unique Swa I site located in the deletion. The Ad17 sequences in the ITR cassette provide regions of homology with the purified Ad24 viral DNA in which recombination can occur. The ITR cassette was also designed to contain unique restriction enzyme sites (Pme I) located at the end of the viral ITR&#39;s so that digestion will release the Ad24 genome from plasmid sequences. Potential clones were screened by restriction analysis and one clone was selected as pAd24ΔE1. pAd24ΔE1 contains Ad17 sequences from bp 4 to 414 and from bp 3373 to 4580, Ad24 bp 4588 to 34529, and Ad17 bp 34469 to 35098 (bp numbers refer to the wt sequence for both Ad17 and Ad24). PAd24ΔE1 contains the coding sequences for all Ad24 virion structural proteins that constitute its serotype specificity. This approach can be used to circularize any group D serotype into plasmid form which has sufficient homology to Ad17.  
         [0056]    To determine if pre-adenovirus plasmid pAd24ΔE1 could be rescued into virus and propagated in a group C E1 complementing cell line, the plasmid was digested with Pme I and transfected into a 6 cm dish of 293 cells using the calcium phosphate co-precipitation technique. Pme I digestion releases the viral genome from the plasmid sequences allowing viral replication to occur after entry into 293 cells. Viral cytopathic effect (CPE), indicating that virus replication and amplification is occurring, was very slow to arise. Following multiple attempts, we were successful at rescuing and amplifying Ad24ΔE1 but the virus grew to lower titers and took more passages to amplify than a similar Ad5 based vector. In order to verify the genetic structure of the virus, viral DNA was extracted using pronase treatment followed by phenol chloroform extraction and ethanol precipitation. Viral DNA was then digested with HindIII and treated with Klenow fragment to end-label the restriction fragments with P33-dATP. The end-labeled restriction fragments were then size-fractionated by gel electrophoresis and visualized by autoradiography. The digestion products were compared with the digestion products from the pre-plasmid (that had been digested with Pme1/HindIII prior to labeling). The expected sizes were observed, indicating that the virus had been successfully rescued.  
       EXAMPLE 2  
       [0057]    Insertion of Ad5 Orf 6 into the E1 region of Ad24  
         [0058]    In order to determine if the insertion of Ad5 E4 Orf6 into the Ad24 genome would allow more efficient propagation in a group C E1 complementing cell line we constructed an Ad24 based pre-adenovirus plasmid containing Ad5 Orf6 in the E1 region. In order to introduce Ad5 Orf6 in to the E1 region of pAd24ΔE1, bacterial recombination was used. An Ad5 Orf6 transgene consisting of the Ad5 Orf6 coding region flanked by the HCMV promoter and pA was cloned into the E1 deletion in an Ad17 shuttle vector (a precursor to the Ad17 ITR cassette). The Ad5 Orf6 transgene was cloned between bp 414 and 3373 in the E1 anti-parallel orientation. The shuttle vector containing the Ad5 Orf6 transgene was digested to generate a DNA fragment consisting of the transgene flanked by Ad17 sequences (bp 4 to 414 and bp 3373 to 4580) and the fragment was purified after electrophoresis on an agarose gel. Cotransformation of BJ 5183 bacteria with the shuttle vector fragment and pAd24ΔE1, which had been linearized in the E1 region by digestion with SwaI, resulted in the generation of pAd24ΔE1 Ad5Orf6 by homologous recombination (FIG. 2). Potential clones were screened by restriction analysis and one clone was selected as pre-adenovirus plasmid pAd24ΔE1Ad5Orf6.  
         [0059]    In order to determine if pre-adenovirus plasmid pAd24ΔE1Ad5Orf6 could be rescued into virus and propagated in an Ad5/group C E1 complementing cell line, pAd24ΔE1Ad5Orf6 was digested with Pme I and transfected into a 6 cm dish of 293 cells using the calcium phosphate co-precipitation technique. PmeI digestion releases the viral genome from plasmid sequences allowing viral replication to occur after entry into 293 cells. Once complete viral cytopathic effect (CPE) was observed at approximately 7-10 days post transfection, the infected cells and media were freeze/thawed three times and the cell debris pelleted. The virus was amplified in two additional passages in 293 cells and then purified from the final infection by ultracentrifugation on CsCl density gradients. In order to verify the genetic structure of the virus, viral DNA was extracted using pronase treatment followed by phenol chloroform extraction and ethanol precipitation. Viral DNA was then digested with HindIII and treated with Klenow fragment to end-label the restriction fragments with P33-dATP. The end-labeled restriction fragments were then size-fractionated by gel electrophoresis and visualized by autoradiography. The digestion products were compared with the digestion products from the pre-plasmid (that had been digested with Pme1/HindIII prior to labeling). The expected sizes were observed, indicating that the virus had been successfully rescued.  
       EXAMPLE 3  
       [0060]    Insertion of Ad5 Orf 6 into the E4 region of Ad24  
         [0061]    To refine the strategy of including Ad5 Orf6 in the genome of an alternative serotype so that propagation could take place in an Ad5/group C complementing cell line two additional strategies were developed. In the first strategy, the entire alternative serotype E4 region (not including the E4 promoter) was deleted and replaced with Ad5 Orf6. In the second strategy, just the alternative serotype Orf6 gene was deleted and replaced with Ad5 Orf6. The configuration of the E4 regions generated by the two strategies is diagramed in FIG. 3. For each of these strategies the desired pre-Adenovirus plasmid was generated by bacterial recombination. Cotransformation of BJ 5183 bacteria with pAd24ΔOrf6BstZ17I and the appropriately constructed Ad24 E4 shuttle plasmid resulted in the generation of the desired Ad24 based pre-Ad plasmid. PAd24ΔOrf6BstZ17I, a derivative of pAd24ΔE1, was constructed so that the E4 region in the Ad24 pre-Ad plasmid could be easily modified using bacterial recombination. PAd24ΔOrf6BstZ17I contains a deletion in the E4 region from Ad24 bp 32373 to bp 33328 with a unique BstZ17I site located at the position of the deletion. The complete sequence of pAd24ΔOrf6BstZ17I consists of Ad17 sequences from bp 4 to 414 and from bp 3373 to 4580, Ad24 bp 4588 to 32372 and from 33329 to 34529, and Ad17 bp 34469 to 35098 (bp numbers refer to the wt sequence for both Ad17 and Ad24).  
         [0062]    To construct pAd24ΔE1 ΔE4Ad5Orf6 (An Ad24 pre-Ad plasmid containing an E1 deletion and a deletion of E4 substituted with Ad5 Orf6), an Ad24 E4 shuttle plasmid was constructed by digesting pAd24ΔE1 with PmeI and BsrGI and cloning the restriction fragment representing the E4 region (bp 31559 to bp 35164) into pNEB193, generating pNEBAd24E4. PNEBAd24E4 was then digested with AccI and EcoNI to remove the E4 coding sequences and ligated with an oligo designed to contain BgIII and XhoI sites (underlined) (5′ ACTCGAGATGTATAGATCT (SEQ ID NO: 10); 5° CTAGATCTATACATCTCGAG (SEQ ID NO: 11)), generating pNEBAd24ΔE4. PNEBAd24ΔE4 was then digested with Bg/II and XhoI and ligated with the Ad5 Orf6 gene, which was PCR amplified, generating pNEBAd24ΔE4Ad5Orf6. The PCR primers used to amplify the Ad5 Orf6 gene (5′ GCACAGATCTTTGCTTCAGGAATATG (SEQ ID NO: 12); 5′ GAGAACTCGAGGCCTACATGGGGGTAGAG (SEQ ID NO: 13)) were designed to contain BgII and XhoI sites (underlined above) for ligation with the pNEBAd24DE4 fragment. In the final step pNEBAd24ΔE4Ad5Orf6 E4 shuttle plasmid was digested with PvuI and PmeI, the restriction fragments were size fractionated by agarose gel electrophoresis and the desired fragment containing Ad5Orf6 flanked by Ad24 sequences was gel purified. Cotransformation of BJ 5183 bacteria with E4 shuttle fragment and pAd24ΔOrf6BstZ17I, which had been linearized in the E4 region by digestion with BstZ17I, resulted in the generation of pAd24ΔE1ΔE4Ad5Orf6 by homologous recombination. Potential clones were screened by restriction analysis and one clone was selected as pre-adenovirus plasmid pAd24ΔE1ΔE4Ad5Orf6.  
         [0063]    To construct pAd24ΔE1ΔOrf6Ad5Orf6 (An Ad24 pre-Ad plasmid containing an E1 deletion and a deletion of E4 Orf6 substituted with Ad5 Orf6), an Ad24 E4 shuttle plasmid was constructed in which the Ad24 Orf6 gene was replaced by Ad5 Orf6. To do this the EcoR1 restriction fragment representing bp 32126 to bp 33442 of the Ad24 genome (encompassing the E4 Orf6 coding region), was subcloned into the EcoRI site in pNEB193, generating pNEBAd240rf6. In order to delete the E4 Orf6 gene in pNEBAd240rf6 and replace it with Ad5 Orf6, pNEBAd24Orf6 was digested with Styl and treated with Klenow to blunt the ends and then digested with to EagI. The desired pNEBAd24Orf6 fragment was then ligated with a PCR product representing the Ad5 Orf6 gene from Ad5 bp 33193 to bp 24125, generating pNEBAd24ΔOrf6Ad5Orf6. The PCR primers used to generate the Ad5 Orf6 fragment (5′CGAGACGGCCGACGCAGATCTGTTTG (SEQ ID NO: 14); 5′GAAGTCCCGGGCTACATGGGGGTAG (SEQ ID NO: 15)) were designed to contain EagI and Smal sites (underlined above) for ligation with the pNEBAd24Orf6 fragment. In the final step pNEBAd24ΔOrf6Ad5Orf6 was digested with EcoRI, the restriction fragments were size fractionated by agarose gel electrophoresis and the desired fragment containing Ad5Orf6 flanked by Ad24 sequences was gel purified. Cotransformation of BJ 5183 bacteria with the EcoRI fragment and pAd24ΔOrf6BstZ17I, which had been linearized in the E4 region by digestion with BstZ17I, resulted in the generation of pAd24ΔE1ΔOrf6Ad5Orf6 by homologous recombination. Potential clones were screened by restriction analysis and one clone was selected as pre-adenovirus plasmid pAd24ΔE1ΔOrf6Ad5Orf6.  
       EXAMPLE 4  
       [0064]    Rescue of pAd24ΔE1ΔE4Ad5Orf6, pAd24ΔE1ΔOrf6Ad5Orf6, into Virus  
         [0065]    In order to determine if pre-adenovirus plasmids pAd24ΔE1ΔE4Ad5Orf6, pAd24ΔE1ΔOrf6Ad5Orf6, could be rescued into virus and propagated in a group C E1 complementing cell line, the plasmids were each digested with Pme I and transfected into T-25 flasks of PER.C6 cells using the calcium phosphate co-precipitation technique; (Cell Phect Transfection Kit, Amersham Pharmacia Biotech Inc.). PmeI digestion releases the viral genome from plasmid sequences allowing viral replication to occur after cell entry. Viral cytopathic effect (CPE), indicating that virus replication and amplification was occurring, was observed for both constructs. When CPE was complete, approximately 7-10 days post transfection, the infected cells and media were harvested, freeze/thawed three times and the cell debris pelleted by centrifugation. Approximately 1 ml of the cell lysate was used to infect T-225 flasks of PER.C6 cells at 80-90% confluence. Once CPE was reached, infected cells and media were harvested, freeze/thawed three times and the cell debris pelleted by centrifugation. Clarified cell lysates were then used to infect 2-layer NUNC cell factories of PER.C6 cells. Following complete CPE the virus was purified by ultracentrifugation on CsCl density gradients. In order to verify the genetic structure of the rescued viruses, viral DNA was extracted using pronase treatment followed by phenol chloroform extraction and ethanol precipitation. Viral DNA was then digested with HindIII and treated with Klenow fragment to end-label the restriction fragments with P33-dATP. The end-labeled restriction fragments were then size-fractionated by gel electrophoresis and visualized by autoradiography. The digestion products were compared with the digestion products of the corresponding pre-Adenovirus plasmid (that had been digested with Pme1/HindIII prior to labeling) from which they were derived. The expected sizes were observed, indicating that the viruses had been successfully rescued.  
       EXAMPLE 5  
       [0066]    Comparison of the growth kinetics Ad24 based vectors.  
         [0067]    In order to compare the growth kinetic of Ad24ΔE1, Ad24ΔE1Ad5Orf6, Ad24ΔE1ΔE4Ad5Orf6 and Ad24ΔE1ΔOrf6Ad5Orf6 one step growth curves were preformed (FIG. 4). PER.C6 cells in 60 mm dishes were infected at 1 vp per cell with either Ad24ΔE1, Ad24ΔE1Ad5Orf6, Ad24ΔE1ΔE4Ad5Orf6 or Ad24ΔE1ΔOrf6Ad5Orf6. Cells and media were then harvested at various times post infection, freeze thawed three times and clarified by centrifugation. The amount of virus present in the samples was determined by quantitative PCR and is illustrated in FIG. 4. This study demonstrates that Ad24 vectors that incorporate Ad5 Orf6 have a distinct growth advantage over Ad24ΔE1 in PER.C6 cells. The instant invention can be practiced with recombinant Ad24 vectors absent a heterologous Orf 6 region where the E1-complementing cell line expresses an Ad24 E1 region or, alternatively, E1 and E4 regions of the same serotype (such as Ad5E1/E4-expressing cell lines).  
       EXAMPLE 6  
       [0068]    Insertion of an Expression Cassette into pAd24ΔE1ΔE4Ad5Orf6, pAd24ΔE1ΔOrf6Ad5Orf6,  
         [0069]    In order to introduce a gag or SEAP expression cassette (see FIGS. 13 and 14, respectively) into the E1 region of the Ad24 pre-Adenovirus plasmids described above (pAd24ΔE1ΔE4Ad5Orf6, pAd24ΔE1ΔOrf6Ad5Orf6) bacterial recombination was used. A gag expression cassette consisting of the following: 1) the immediate early gene promoter from the human cytomegalovirus, 2) the coding sequence of the human immunodeficiency virus type 1 (HIV-1) gag (strain CAM-1; 1526 bp) gene, and 3) the bovine growth hormone polyadenylation signal sequence, was cloned into the E1 deletion in Ad17 shuttle plasmid, pABSAd17-3, generating pABSAd17HCMVgagBGHpA. The ITR cassette contains sequences from the right (bp 34469 to 35098) and left (bp 4 to 414 and bp 3373 to 4580) end of the Ad17 genome separated by plasmid sequences containing a bacterial origin of replication and an Ampicillin resistance gene. The ITR cassette contains a deletion of E1 sequences from Ad17 (bp 415 to 3372) with a unique Swa I site located in the deletion. The gag expression cassette was obtained from a previously constructed shuttle plasmid by EcoRI digestion. Following the digestion the desired fragment was gel purified, treated with Klenow to obtain blunt ends and cloned into the SwaI site in pABSAd17-3. This cloning step resulted in the gag expression cassette being cloned into the E1 deletion between bp 414 and 3373 in the E1 parallel orientation. The shuttle vector containing the gag transgene was digested to generate a DNA fragment consisting of the gag expression cassette flanked by Ad17 bp 4 to 414 and bp 3373 to 4580 and the fragment was purified after electrophoresis on an agarose gel. Cotransformation of BJ 5183 bacteria with the shuttle vector fragment and one of the Ad24 pre-Ad plasmids (pAd24ΔE1ΔE4Ad5Orf6, pAd24ΔE1ΔOrf6Ad5Orf6,), linearized in the E1 region by digestion with Swa I, resulted in the generation of the corresponding Ad24 gag-containing pre-Adenovirus plasmids (pAd24ΔE1gagΔE4Ad5Orf6, pAd24ΔE1gagΔOrf6Ad5Orf6) by homologous recombination. Potential clones were screened by restriction analysis.  
         [0070]    A similar strategy was used to generate Ad24 pre-Ad plasmids containing a SEAP expression cassette. In this case a SEAP expression cassette consisting of: 1) the immediate early gene promoter from the human cytomegalovirus, 2) the coding sequence of the human placental SEAP gene, and 3) the bovine growth hormone polyadenylation signal sequence was cloned into the E1 deletion in Ad17 shuttle plasmid, pABSAd17-3, generating pABSAd17HCMVSEAPBGH. The SEAP expression cassette was obtained from a previously constructed shuttle plasmid by EcoRI digestion. Following the digestion the desired fragment was gel purified, treated with Klenow to obtain blunt ends and cloned into the SwaI site in pABSAd17-3. The shuttle vector containing the SEAP transgene was digested to generate a DNA fragment consisting of the SEAP expression cassette flanked by Ad17 bp 4 to 414 and bp 3373 to 4580 and the fragment was purified after electrophoresis on an agarose gel. Cotransformation of BJ 5183 bacteria with the shuttle vector fragment and one of the Ad24 pre-Ad plasmids (pAd24ΔE1ΔE4Ad5Orf6, pAd24ΔE1ΔOrf6Ad5Orf6,), linearized in the E1 region by digestion with Swa I, resulted in the generation of the corresponding Ad24 SEAP-containing pre-Adenovirus plasmids (pAd24ΔE1SEAPΔE4Ad5Orf6, pAd24ΔE1SEAPΔOrf6Ad5Orf6) by homologous recombination. Potential clones were screened by restriction analysis. All pre-Ad plasmids were rescued into virus and expanded to prepare CsCl purified stocks as described above.  
       EXAMPLE 7  
       [0071]    In Vivo Immunogenicity  
         [0072]    A. Immunization  
         [0073]    Cohorts of 3-6 animals were given intramuscular injections at wk 0 and wk 4 of either of the following constructs: (1) 10{circumflex over ( )}11 vp MRKAd5-HIV1 gag; (2) 10{circumflex over ( )}10 vp MRKAd5-HIV1gag; (3) 10{circumflex over ( )}11 vp of Ad24ΔE1gagΔOrf6Ad5Orf6; (4) 10 {circumflex over ( )}10 vp of Ad24ΔE1gagΔOrf6Ad5Orf6; or (5) 10{circumflex over ( )}10 vp of Ad24ΔE1gagΔE4Ad5Orf6. Rhesus macaques were between 3-10 kg in weight. In all cases, the total dose of each vaccine was suspended in 1 mL of buffer. The macaques were anesthetized (ketamine/xylazine) and the vaccines were delivered i.m. in 0.5-mL aliquots into both deltoid muscles using tuberculin syringes (Becton-Dickinson, Franklin Lakes, N.J.). Peripheral blood mononuclear cells (PBMC) were prepared from blood samples collected at several time points (typically 4 wk intervals) during the immunization regimen. All animal care and treatment were in accordance with standards approved by the Institutional Animal Care and Use Committee according to the principles set forth in the  Guide for Care and Use of laboratory Animals , Institute of Laboratory Animal Resources, National Research Council.  
         [0074]    B. ELISPOT Assay  
         [0075]    The IFN-γ ELISPOT assays for rhesus macaques were conducted following a previously described protocol (Allen et al., 2001  J Virol . 75(2):738-749; Casimiro et al., 2002  J Virol . 76:185-94), with some modifications. For antigen-specific stimulation, a peptide pool was prepared from 20-aa peptides that encompass the entire HIV-1 gag sequence with 10-aa overlaps (Synpep Corp., Dublin, Calif.). To each well, 50 μL of 2-4×10 5  peripheral blood mononuclear cells (PBMCs) were added; the cells were counted using Beckman Coulter Z2 particle analyzer with a lower size cut-off set at 80 femtoliters (“fL”). Either 50 μL of media or the gag peptide pool at 8 μg/mL concentration per peptide were added to the PBMC. The samples were incubated at 37° C., 5% CO 2  for 20-24 hrs. Spots were developed accordingly and the plates were processed using custom-built imager and automatic counting subroutine based on the ImagePro platform (Silver Spring, MD); the counts were normalized to 10 6  cell input.  
         [0076]    C. Intracellular Cytokine Staining.  
         [0077]    To 1 ml of 2×10 6  PBMC/mL in complete RPMI media (in 17×100 mm round bottom polypropylene tubes (Sarstedt, Newton, N.C.)), anti-hCD28 (clone L293, Becton-Dickinson) and anti-hCD49d (clone L25, Becton-Dickinson) monoclonal antibodies were added to a final concentration of 1 μg/mL. For gag-specific stimulation, 10 μL of the peptide pool (at 0.4 mg/mL per peptide) were added. The tubes were incubated at 37° C. for 1 hr., after which 20 μL of 5 mg/mL of brefeldin A (Sigma) were added. The cells were incubated for 16 hr at 37° C., 5% CO 2 , 90% humidity. 4 mL cold PBS/2% FBS were added to each tube and the cells were pelleted for 10 min at 1200 rpm. The cells were re-suspended in PBS/2% FBS and stained (30 min, 4° C.) for surface markers using several fluorescent-tagged mAbs: 20 μL per tube anti-hCD3-APC, clone FN-18 (Biosource); 20 μL anti-hCD8-PerCP, clone SK1 (Becton Dickinson); and 20 μL anti-hCD4-PE, clone SK3 (Becton Dickinson). Sample handling from this stage was conducted in the dark. The cells were washed and incubated in 750 μL 1×FACS Perm buffer (Becton Dickinson) for 10 min at room temperature. The cells were pelleted and re-suspended in PBS/2% FBS and 0.1 μg of FITC-anti-hIFN-γ, clone MD-1 (Biosource) was added. After 30 min incubation, the cells were washed and re-suspended in PBS. Samples were analyzed using all four color channels of the Becton Dickinson FACSCalibur instrument. To analyze the data, the low side- and forward-scatter lymphocyte population was initially gated; a common fluorescence cut-off for cytokine-positive events was used for both CD4 +  and CD8 +  populations, and for both mock and gag-peptide reaction tubes of a sample.  
         [0078]    D. Anti-p24 ELISA.  
         [0079]    A modified competitive anti-p24 assay was developed using reagents from the Coulter p24 Antigen Assay kit (Beckman Coulter, Fullerton, Calif.). Briefly, to a 250-μL serum sample, 20 μL of Lyse Buffer and 15 μL of p24 antigen (9.375 pg) from the Coulter kit were added. After mixing, 200 μL of each sample were added to wells coated with a mouse anti-p24 mAb from the Coulter kit and incubated for 1.5 hr at 37° C. The wells were then washed and 200 μL of Biotin Reagent (polyclonal anti-p24-biotin) from the Coulter kit was added to each well. After a 1 hr, 37° C. incubation, detection was achieved using strepavidin-conjugated horseradish peroxidase and TMB substrate as described in the Coulter Kit. OD 450nm  values were recorded. A 7-point standard curve was generated using a serial 2-fold dilution of serum from an HIV-seropositive individual. The lower cut-off for the assay is arbitrarily set at 10 milli Merck units/mL (mMU/mL) defined by a dilution of the seropositive human serum. This cutoff falls at approximately 65% of the maximum bound control signal which corresponds to that obtained with the diluent control only and with no positive analyte.  
         [0080]    E. Results  
         [0081]    PBMCs collected at regular 4-wk intervals were analyzed in an ELISPOT assay (FIG. 6). Both Ad24ΔE1gagΔOrf6Ad5Orf6 and Ad24ΔE1gagΔE4Ad5Orf6 were able to induce significant levels of gag-specific T cells in non-human primates. At 10{circumflex over ( )}11 vp dose level, the Ad24-induced responses were within 2-3-fold of those of MRKAd5-HIV1 gag. Both Ad24 vectors were also able to induce detectable levels of gag-specific T cells at 10{circumflex over ( )}10 vp but were lower than those observed using MRKad5gag at the same dose.  
         [0082]    PBMCs collected at wk 12 from the vaccinees were analyzed for intracellular IFN-γ staining after the priming immunizations. The assay results provided information on the relative amounts of CD4 +  and CD8 +  gag-specific T cells in the peripheral blood (FIG. 7). The results indicated that the prime-boost immunization approach was able to elicit in rhesus macaques both HIV-specific CD4 +  and CD8 +  T cells.  
         [0083]    F. Humoral Immune Responses.  
         [0084]    The Ad24-based vaccine vector was able to generate detectable levels of circulating anti-gag antibodies at the reasonably high dose level (FIG. 8). No detectable titers were observed at equal to or lower than 10{circumflex over ( )}10 vp, suggesting the existence of a dose-dependent response.  
       EXAMPLE 8  
       [0085]    In Vivo Transgene Expression  
         [0086]    A. Immunization  
         [0087]    Cohorts of 5 C3H/HeN mice were given single intramuscular injections of one of the following vectors: (1) 10{circumflex over ( )}10 vp Ad24ΔE1SEAPΔE4Ad5Orf6; (2) 10{circumflex over ( )}10 vp Ad24ΔE1SEAPΔOrf6Ad5Orf6; (3) 10{circumflex over ( )}10 vp MRKAd5SEAP; and (4) 10{circumflex over ( )}9 vp MRKAd5SEAP. Female mice were between 4-10 weeks old. The total dose of each vaccine was suspended in 0.1 mL of buffer. The vectors were given to both quadriceps of each of the animals with a volume of 50 uL per quad and using 0.3-mL 28G1/2 insulin syringes (Becton-Dickinson, Franklin Lakes, N.J.). For the primates, the total dose of each vaccine was suspended in 1 mL of buffer. The monkeys were anesthetized (ketamine/xylazine mixture) and the vaccines were delivered i.m. in 0.5-mL aliquots into two muscle sites using tuberculin syringes (Becton-Dickinson, Franklin Lakes, N.J.). Serum samples were collected at defined intervals and stored frozen until the assay date. All animal care and treatment were in accordance with standards approved by the Institutional Animal Care and Use Committee according to the principles set forth in the Guide for Care and Use of Laboratory Animals, Institute of Laboratory Animal Resources, National Research Council.  
         [0088]    B. SEAP Assay  
         [0089]    Serum samples were analyzed for circulating SEAP levels using TROPIX phospha-light chemiluminescent kit (Applied Biosystems Inc). Duplicate 5 uL aliquots of each serum were mixed with 45 uL of kit-supplied dilution buffer in a 96-well white DYNEX plate. Serially diluted solutions of a human placental alkaline phosphatase (Catalog no. M5905, Sigma, St. Louis, Mo.) in 10% naïve monkey serum served to provide the standard curve. Endogenous SEAP activity in the samples was inactivated by heating the wells for 30 minutes at 65° C. Enzymatic SEAP activities in the samples were determined following the procedures described in the kit. Chemiluminescence readings (in relative light units) were recorder using DYNEX luminometer. RLU readings are converted to ng/mL SEAP using a log-log regression analyses.  
         [0090]    C. Rodent Results  
         [0091]    Serum samples prior to and after the injection were analyzed for circulating SEAP activities and the results are shown in FIG. 9. Results indicate that (1) both Ad24 constructs are all capable of expressing the SEAP transgene in vivo to comparable levels; and that (2) the level of expression achieved using the Ad24 vectors are comparable to that of Ad5 at 10-fold lower dose. The levels of SEAP in the serum dropped dramatically after day 2 and were at background levels by day 12.  
         [0092]    D. Primate Results  
         [0093]    Cohorts of 3 rhesus macaques were given single intramuscular injections of one of the following vectors: (1) 10{circumflex over ( )}11 vp MRKAd5-SEAP; (2) 10{circumflex over ( )}9 vp MRKAd5-SEAP; (3) 10{circumflex over ( )}11 vp Ad24ΔE1SEAPΔOrf6Ad5Orf6; or (4) 10{circumflex over ( )}11 vp Ad24ΔE1SEAPΔE4Ad5Orf6. Serum samples prior to and after the injection were analyzed for circulating SEAP activities and the results are shown in FIG. 10.  
         [0094]    Results indicate that the peak levels of SEAP product produced by adenovirus serotype 24 were lower than but were within 3-fold of that of MRKAd5 at the same high dose level of 10{circumflex over ( )}11 vp (FIG. 10). The levels observed with adenovirus serotype 24 are generally 50-fold higher than those observed using 10{circumflex over ( )}9 vp of MRKAd5. The levels of SEAP in the serum dropped dramatically after day 10 and were close to background as early as day 15. These observations strongly indicate that adenovirus serotype 24 is very efficient in expressing a transgene following intramuscular administration in a primate.  
       EXAMPLE 9  
       [0095]    Construction of pMRKAd24ΔE1ΔE4Ad5Orf6  
         [0096]    To construct pMRKAd24ΔE1ΔE4Ad5Orf6 (An Ad24 pre-Ad plasmid, composed entirely of Ad24 sequence and containing an E1 deletion and an E4 deletion substituted with Ad5 Orf6), an Ad24 ITR cassette was constructed containing sequences from the right (bp 31978 to 32264 and bp 34713 to 35164) and left (bp 4 to 450 and bp 3364 to 3799) end of the Ad24 genome separated by plasmid sequences containing a bacterial origin of replication and an ampicillin resistance gene. These four segments were generated by PCR and cloned sequentially into pNEB193, generating pNEBAd24-4. Next the Ad5 Orf6 open reading frame (Ad5 bp 31192 to bp 34078) was generated by PCR and cloned between Ad24 bp 32264 and 34713 generating pNEBAd24E-Ad5Orf6 (the ITR cassette). PNEB193 is a commonly used commercially available cloning plasmid (New England Biolabs cat# N3051S) containing a bacterial origin of replication, ampicillin resistance gene and a multiple cloning site into which the PCR products were introduced. The ITR cassette contains a deletion of E1sequences from Ad24 bp 451 to 3363 with a unique Swa I restriction site located in the deletion and an E4 deletion from Ad24 bp 32265 to 34712 into which Ad5 Orf6 was introduced in an E4 parallel orientation. In this construct Ad5 Orf6 expression is driven by the Ad24 E4 promoter. The Ad24 sequences (bp 31978 to 32264 and bp 3464 to 3799) in the ITR cassette provide regions of homology with the purified Ad24 viral DNA in which bacterial recombination can occur following cotransformation into BJ 5183 bacteria (FIG. 11). The ITR cassette was also designed to contain unique restriction enzyme sites (PmeI) located at the end of the viral ITR&#39;s so that digestion will release the recombinant Ad24 genome from plasmid sequences. Potential clones will be screened by restriction analysis and one clone was selected as pMRKAd24ΔE1ΔE4Ad5Orf6. Pre-Adenovirus plasmid pMRKAd24ΔE1ΔE4Ad5Orf6 should contain Ad24 sequences from bp 4 to 450; bp 3364 to bp 32264 and bp 34713 to bp 35164 with Ad5Orf6 cloned between bp 32264 and bp 34713. The bp numbering in the above description refers to the wt sequence for both Ad24 and Ad5.  
       EXAMPLE 10  
       [0097]    Insertion of HIV-1 gag and SEAP transgenes into pAd24ΔE1ΔE4Ad5Orf6  
         [0098]    In order to introduce a gag or SEAP expression cassettes into the E1 region of pMRKAd24ΔE1ΔE4Ad5Orf6, bacterial recombination will be used. An HIV-1 gag expression cassette will consist of the following: 1) the immediate early gene promoter from the human cytomegalovirus, 2) the coding sequence of the human immunodeficiency virus type 1 (HIV-1) gag (strain CAM-1; 1526 bp) gene, and 3) the bovine growth hormone polyadenylation signal sequence, in the E1 deletion of an Ad24 shuttle plasmid, pNEBAd24-2 (a precursor to the Ad24 ITR cassette described above), generating pNEBAd24CMVgagBGHpA. PNEBAd24-2 contains Ad24 sequences from the left end of the genome (bp 4 to 450 and bp 3364 to 3799) that define the E1 deletion. The gag expression cassette will be obtained from a previously constructed plasmid and cloned into the E1 deletion between bp 450 and 3364 in the E1 parallel orientation. The shuttle vector containing the gag transgene will be digested to generate a DNA fragment consisting of the gag expression cassette flanked by Ad24 bp 4 to 450 and bp 3364 to 3799 and the fragment will be purified after electrophoresis on an agarose gel. Cotransformation of BJ 5183 bacteria with the shuttle vector fragment and pMRKAd24ΔE1 ΔE4Ad5Orf6 which was linearized in the E1 region by digestion with SwaI, should result in the generation of Ad24 gag-containing pre-Adenovirus plasmids pMRKAd24ΔE1gagΔE4Ad5Orf6 by homologous recombination. Potential clones will be screened by restriction analysis.  
         [0099]    A similar strategy will be used to generate Ad24 pre-Ad plasmids containing a SEAP expression cassette. In this case, a SEAP expression cassette will consist of: 1) the immediate early gene promoter from the human cytomegalovirus, 2) the coding sequence of the human placental SEAP gene, and 3) the bovine growth hormone polyadenylation signal sequence cloned into the E1 deletion of an Ad24 shuttle plasmid, pNEBAd24-2, generating pNEBAd24CMVSEAPBGHpA. The transgene will then be recombined into pMRKAd24ΔE1ΔE4Ad5Orf6 as described above for the gag transgene.  
       EXAMPLE 11  
       [0100]    In Vivo Immunogenicity  
         [0101]    A. Immunization  
         [0102]    Rhesus macaques were between 3-10 kg in weight. In all cases, the total dose of each vaccine was suspended in 1 mL of buffer. The macaques were anesthetized (ketamine/xylazine) and the vaccines were delivered i.m. in 0.5-mL aliquots into both deltoid muscles using tuberculin syringes (Becton-Dickinson, Franklin Lakes, N.J.). Peripheral blood mononuclear cells (PBMC) were prepared from blood samples collected at several time points during the immunization regimen. All animal care and treatment were in accordance with standards approved by the Institutional Animal Care and Use Committee according to the principles set forth in the  Guide for Care and Use of Laboratory Animals , Institute of Laboratory Animal Resources, National Research Council.  
         [0103]    B. T Cell Responses  
         [0104]    Ad24 Vaccine Vector as a Heterologous Booster: Cohort of 4 rhesus macaques. was immunized initially with 3 doses (wk 0, 4, 26) of either 10 7  or 10 9  vp of MRKAd5-gag (see FIGS.  16 A-16AX) or MRKAd6-gag(see FIGS.  17 A-17N). At wk 56, the animals received a booster vaccine of 10 11  vp Ad24ΔE1gagΔOrf6Ad5Orf6. A separate cohort of naïve animals received a single dose of the booster vaccine. The results of the IFN-γ ELISPOT analyses of PBMC collected during the course of the studies are shown in FIG. 18. It is apparent that the Ad24 HIV vectors can be utilized to amplify the existing pools of HIV-specific T cells. The increases in the levels of gag-specific T cells from the pre-boost levels to those measured at 4 wks post boost were consistently larger than the levels induced by the same booster vaccine in naïve animals. PBMCs from the vaccinees of the heterologous MRKAd5/MRKAd6-Ad24 boost regimen were analyzed for intracellular IFN-γ staining after the priming immunizations (wk 60). The assay results provided information on the relative amounts of CD4 +  and CD8 +  gag-specific T cells in the peripheral blood (FIG. 19). The results indicated that heterologous prime-boost immunization approach was able to elicit in rhesus macaques both HIV-specific CD4 +  and CD8+ T cells.  
         [0105]    Ad24 Vaccine Vector as a Heterologous Primer: In a separate study, a cohort of 3 rhesus macaques was immunized initially with 2 doses (wk 0, 4) of 10 11  vp Ad24ΔE1gagΔOrf6Ad5Orf6 and boosted at wk 24 with 10 7  vp of MRKAd5-gag. The low dose of MRKAd5-gag is selected to mimic the effect of pre-existing neutralizing immunity to the vector in a subject. A separate cohort of naïve animals was given a single dose of 10 7  Vp MRKAd5-gag. The results of the IFN-γ ELISPOT analyses of PBMC collected during the course of the studies are shown in FIG. 20. 5 The Ad24-based vaccine was able to prime effectively for HIV-specific T cell responses in macaques. Boosting with a low dose MRKAd5-gag resulted in a significant increase in the levels of gag-specific T cells. The increases in 2 out of 3 animals exceed the levels typically observed after treatment of naïve animals with the same low dose of MRKAd5-gag.  
     
       
       
         1 
         
           
             15  
           
           
             1  
             35167  
             DNA  
             adenovirus serotype 24  
           
            1 

catcatcaat aatatacccc acaaagtaaa caaaagttaa catgcaaatg agcttttgaa     60 

tttagggcgg ggccagcgct gattggacga gagaagatga tgcaaatgac gtcacgacgc    120 

acggctaacg gtcgccgcgg aggcgtggcc tagcccggaa gcaagtcgcg gggctgatga    180 

cgtataaaaa agcggacttt agacccggaa acggccgatt ttcccgcggc cacgcccgga    240 

tatgaggtaa ttctgggcgg atgcaagtaa aattaggtca ttttggcgcg aaaactgaat    300 

gaggaagtga aaagtgaaaa ataccggtcc cgcccagggc ggaatattta ccgagggccg    360 

agagactttg accgattacg tgggggtttc gattgcggtg ttttttcgcg aatttccgcg    420 

tccgtgtcaa agtccggtgt ttatgtcaca gatcagctga tccacagggt atttaaacca    480 

gtcgagcccg tcaagaggcc actcttgagt gccagcgagt agagatttct ctgagctccg    540 

ctcccagagt ctgagaaaaa tgagacacct gcgcctcctt tcttcaactg tgcctattga    600 

catggccgca ttattgctgg aggattatgt gagtacaata ttggaggacg aactgcatcc    660 

atctccattt gagctgggac ctacacttca ggacctatat gatttggagg tagatgccca    720 

tgatgacgac ccgaacgaag aggctgtgaa tttaatattt ccagaatctc tgattcttca    780 

ggctgacata gccagcgaag ctgtacctac accacttcat acaccgactc tgtcacccat    840 

acctgaattg gaagaggagg acgagctaga cctccgatgt tatgaggaag gttttcctcc    900 

cagcgattca gaggacgaac agggtgagca gagcatggct ctaatctcaa aatatgcttg    960 

tgtggttgtg gaagagcatt ttgtgttgga caatcctgag gtgcccgggc aaggctgtag   1020 

atcctgccag taccaccggg ataagaccgg agacacgaac gcctcctgcg ctctgtgtta   1080 

catgaaaaag aacttcagct ttatttacag taagtggagt gaatgtgaga gagactgagt   1140 

gcttaacaca taactgggta atgcttaaac agctgtgcta agtgtggttt atttttgttt   1200 

ctaggtccgg tgtcagagga tgagtcatca ccctcagaag aagaccaccc gtgtccccct   1260 

gagctgtcag gcgaaacgcc cctgcaagtg cacagaccca ccccagtcag acccagtggc   1320 

gagaggcgag cagctgttga aaaaattgag gacttgttac atgacatggg tggggatgaa   1380 

cctttggacc tgagcttgaa acgccccagg aactaggctc agctgtgctt agtcatgtgt   1440 

aaataaagtt gtacaataaa agtatatgtg acgcatgcaa ggtgtggttt atgactcatg   1500 

ggcgtggctt agtcctatat aagtggcaac acctgggcac tggggcacag accttcaggg   1560 

agttcctgat ggatgtgtgg actatccttg cagactttag caagacacgc cggcttgtag   1620 

aggatagttc agacgggtgc tccgggttct ggagacactg gtttggaact cctctatctc   1680 

gtctggtgta cacagttaag aaggattata acgaggaatt tgaaaatctt tttgctgatt   1740 

gctctggcct gctagattct ctaaatctcg gccaccagtc ccttttccag gaaagggtac   1800 

tccacagcct tgatttttca agcccagggc gcactacagc cggggttgct tttgtggttt   1860 

ttctggttga caaatggagc cagaacaccc aactgagcag gggctacatt ctggacttcg   1920 

cagccatgca cctgtggagg gcatgggtga ggcagcgggg acagagaatc ttgaactact   1980 

ggcttataca gccagcagct ccgggtcttc ttcgtctaca cagacaaaca tccatgttgg   2040 

aggaagaaat gaggcaggcc atggacgaga acccgaggag cggcctggac cctccgtcgg   2100 

aagaggagct ggattgaatc aggtatccag cctgtaccca gagcttagca gggtgctgac   2160 

atccatggcc aggggagtga agagggagag gagcgatggg ggcaataccg ggatgatgac   2220 

cgagctgacg gccagcctga tgaatcgcaa gcgtccagag cgcattacct ggcacgagct   2280 

acagatggag tgtagggatg aggtgggcct gatgcaggat aaatatggcc tggagcagat   2340 

aaaaacccac tggttgaacc cagatgagga ttgggaggag gccattaaga aatatgccaa   2400 

gatagccctg cgcccagatt gcaagtacag ggtgaccaag acggtgaata tcagacatgc   2460 

ctgctacatc tcggggaacg gggcagaggt ggtcatcgat accctggaca aggccgcctt   2520 

caggtgttgc atgatgggaa tgagagccgg agtgatgaat atgaattcca tgattttcat   2580 

gaacatgaag ttcaatggag agaagtttaa tggggtgatg ttcatggcca acagtcacat   2640 

gaccctgcac ggctgcagtt tcttcggctt caacaatatg tgcgcagagg tctggggcgc   2700 

tgctaagatc aggggatgta agttttatgg ctgctggatg ggcgtggtcg gaagacccaa   2760 

gagcgagatg tctgtgaagc agtgtgtgtt tgagaaatgc tacctgggag tctctaccga   2820 

gggcaatgct agagtgagac attgctcttc cctggagacg ggctgcttct gcctggtgaa   2880 

gggcacagcc tctctgaagc ataatatggt gaagggctgc acggatgagc gcatgtacaa   2940 

catgctgaca tgcgactcgg gggtctgcca tatcctgaag aacatccatg tgacctccca   3000 

cccccggaag aagtggccag tgtttgagaa taacctactg atcaagtgcc acatgcacct   3060 

gggcgccaga aggggcacct tccagccgta ccagtgcaac tttagccaga ccaagctgct   3120 

gctggagaac gatgccttct ccagggtgaa cctgaacggc atctttgaca tggatgtctc   3180 

ggtgtacaag atcctgagat acgatgagac caagtccagg gtgcgcgctt gcgagtgcgg   3240 

gggcagacac accaggatgc aaccagtggc cctggatgtg accgaggagc tgaggcccga   3300 

ccacctggtg atggcttgta ccgggaccga gttcagctcc agtggggagg acacagatta   3360 

gaggtaggtt gagtattagt gggcgtggct aaggtgacta taaaggcggg tgtcttacga   3420 

gggtcttttt gcttttctgc agacatcatg aacgggactg gcggggcctt cgaagggggg   3480 

ctttttagcc cttatttgac aacccgcctg ccgggatggg ccggagttcg tcagaatgtg   3540 

atgggatcga cggtggacgg gcgtccagtg cttccagcaa attcctcgac catgacctac   3600 

gcgaccgtgg ggaactcgtc gctcgacagc accgccgcag ccgcggcagc cgcagccgcc   3660 

atgacagcga cgagactggc ttcgagctac atgcccagca gcagcagtag cccctctgtg   3720 

cccagttcca tcatcgccga ggagaaactg ctggccctgc tggccgagct ggaagccctg   3780 

agccgccagc tggccgccct gacccagcag gtgtccgagc tccgcgaaca gcagcagcag   3840 

caaaataaat gattcaataa acacagattc tgattcaaac agcaaagcat ctttattatt   3900 

tattttttcg cgcgcggtag gccctggtcc acctctcccg atcattgaga gtgcggtgga   3960 

ttttttccag gacccggtag aggtgggatt ggatgttgag gtacatgggc atgagcccgt   4020 

cccgggggtg gaggtagcac cactgcatgg cctcgtgctc tggggtcgtg ttgtagatga   4080 

tccagtcata gcaggggcgc tgggcgtggt gctggatgat gtccttgagg aggagactga   4140 

tggccacggg gagccccttg gtgtaggtgt tggcgaagcg gttgagctgg gagggatgca   4200 

tgcgggggga gatgatgtgg agtttggcct ggatcttgag gttggcgatg ttgccaccca   4260 

gatcccgcct ggggttcatg ttgtgcagga ccaccagaac ggtgtagccc gtgcacttgg   4320 

ggaacttgtc atgcaacttg gaagggaatg cgtgaaagaa tttggagacg cccttgtgcc   4380 

cacccaggtt ttccatgcac tcatccatga tgatggcgat gggcccgtgg gctgcggctt   4440 

tggcaaagac gtttctgggg tcagagacat cgtaattatg ctcctgggtg agatcatcat   4500 

aagacatttt aatgaatttg gggcggaggg tgccagattg ggggacaatg gttccctcgg   4560 

gccccggggc gaagttcccc tcacatattt gcatctccca ggctttcatc tcggaggggg   4620 

ggatcatgtc cacctgcggg gcgatgaaaa aaacggtttc cggggcgggg gtgatgagct   4680 

gcgaggagag caggtttctc aacagctggg acttgccgca cccggtcggg ccgtagatga   4740 

ccccgatgac gggttgcagg tggtagttca aggacatgca gctgccgtcg tcccggagga   4800 

ggggggccac ctcgttgagc atgtctctga cttggaggtt ttcccggacg agctcgccga   4860 

ggaggcggtc cccgcccagc gagagcagct cttgcaggga agcaaagttt ttcaggggct   4920 

tgagcccgtc ggccatgggc atcttggcga gggtctgcga gaggagttcg aggcggtccc   4980 

agagctcggt gacgtgctct acggcatctc gatccagcag acttcctcgt ttcgggggtt   5040 

gggacgactg cgactgtagg gcacgagacg atgggcgtcc agcgctgcca gcgtcatgtc   5100 

cttccagggt ctcagtgtcc gcgtgagcgt ggtctccgtc acggtgaagg ggtgggcccc   5160 

gggctgtgcg cttgcaaggg tgcgcttgag actcatcctg ctggtgctga aacgggcacg   5220 

gtcttcgccc tgcgcgtcgg cgagatagca gttgaccatg agctcgtagt tgagggcctc   5280 

ggcggcgtgg cccttggcgc ggagcttgcc cttggaagag cgcccgcagg cgggacagag   5340 

gagggattgc agggcgtaga gcttgggtgc gagaaagacg gactcggggg cgaaagcatc   5400 

cgctccgcag tgggcgcaga cggtctcgca ctcgaccagc caggtgagct cgggctgctc   5460 

ggggtcaaaa accagttttc ccccgttctt tttgatgcgc ttcttacctc gcgtctccat   5520 

gagtctgtgt ccgcgctcgg tgacaaacag gctgtctgtg tccccgtaga cggacttgat   5580 

gggcctgtcc tgcaggggcg tcccgcggtc ctcctcgtag agaaactcgg accactctga   5640 

gacgaaggcg cgcgtccacg ccaagacaaa ggaggccacg tgcgaggggt agcggtcgtt   5700 

gtccaccagg gggtccacct tttccacggt atgcagacac atgtccccct cctccgcatc   5760 

caagaaggtg attggcttgt aggtgtaggc cacgtgaccc ggggtccccg acgggggggt   5820 

ataaaagggg gcgggtctgt gctcgtcctc actctcttcc gcgtcgctgt ccacgagcgc   5880 

cagctgttgg ggtaggtatt ccctttcgag agcgggcatg acctcggcac tcaggttgtc   5940 

agtttctaga aacgaggagg atttgatgtt ggcttgccct gccgcaatgc tttttaggag   6000 

actttcatcc atctggtcag aaaagactat ttttttattg tcaagcttgg tggcgaagga   6060 

gccatagagg gcgttggaga gaagcttggc gatggatctc atggtctgat ttttgtcacg   6120 

gtcggctcgc tccttggccg cgatgttgag ctggacatac tcgcgcgcga cgcacttcca   6180 

ttcggggaag acggtggtgc gctcgtcggg cacgatcctg acgcgccagc cgcggttatg   6240 

cagggtgacc agatccacgc tggtggccac ctcgccgcgc aggggctcgt tggtccagca   6300 

gaggcgtccg cccttgcgcg agcagaacgg gggcagcaca tcaagcagat gctcgtcagg   6360 

ggggtccgca tcgatggtga agatgcccgg acagagttcc ttgtcaaaat aatcgatttt   6420 

tgaggatgca tcatccaagg ccatctgcca ctcgcgggcg gccagcgctc gctcgtaggg   6480 

gttgaggggc ggaccccagg gcatgggatg cgtcagggcg gaggcgtaca tgccgcagat   6540 

gtcgtagaca tagatgggct ccgagaggat gccgatgtag gtgggataac agcgcccccc   6600 

gcggatgctg gcgcgcacgt agtcatacaa ctcgtgcgag ggggccaaga aggcggggcc   6660 

gagattggtg cgctggggct gctcggcgcg gaagacgatc tggcgaaaga tggcatgcga   6720 

gttggaggag atggtgggcc gttggaagat gttaaagtgg gcatgaggca gacgaaccga   6780 

gtcgcggatg aagtgcgcgt aggagtcttg cagcttggcg acgagctcgg cggtgacgag   6840 

gacgtccatg gcgcagtagt ccagcgtttc gcggatgatg tcataacccg cctctccttt   6900 

cttctcccat agctcgcggt tgagggcgta ctcctcgtca tccttccagt actcccggag   6960 

cgggaatcct cgatcgtccg cacggtaaga gcccagcatg tagaaatggt tcacggcctt   7020 

gtagggacag cagcccttct ccacggggag ggcgtaagct tgagcggcct tgcggagcga   7080 

ggtgtgcgtc agggcgaagg tatccctgac catgactttc aagaactggt acttgaaatc   7140 

cgagtcgtcg cagccgccgt gctcccagag ctcgaaatcg gtgcgcttct tcgagagggg   7200 

gttaggcaga gcgaaagtga cgtcattgaa gagaatcttg cctgcccgcg gcatgaaatt   7260 

gcgggtgatg cggaaagggc ccgggacgga ggctcggttg ttgatgacct gggcggcgag   7320 

gacgatctcg tcgaagccgt tgatgttgtg cccgacgatg tagagttcca tgaatcgcgg   7380 

gcggccttta atgtgcggca gctttttgag ctcctcgtag gtgaggtcct cggggcaatg   7440 

cagtccgtgc tgctcgagcg cccactcctg gagatgtggg ttggcttgca tgaatgaagc   7500 

ccagagctcg cgggccataa gggtctggag ctcgtcgcga aagaggcgga actgctggcc   7560 

cacggccatc ttttctgggg tgacgcagta gaaagtaagg gggtcccgct cccagcgatc   7620 

ccagcgtaag cgcacggcta gatcgcgagc gagggcgacc agctctgggt cccccgagaa   7680 

tttcataacc agcataaagg ggacgagctg cttgccgaag gaccccatcc aggtgtaggt   7740 

ttctacatcg taggtgacaa agagccgctc cgtgcgagga tgagagccga ttgggaagaa   7800 

ctggatttcc tgccaccagt tggacgagtg gctgttgatg tgatgaaagt agaaatcccg   7860 

ccggcgaacc gagcactcgt gctgatgctt gtaaaagcgt ccgcagtact cgcagcgctg   7920 

cacgggctgt acctcatcca cgagatacac agcgcgtccc ttgaggagga acttcaggag   7980 

tggcggccct ggctggtggt tttcatgttc gcctgcgtgg gactcaccct ggggctcctc   8040 

gaggacggag aggctgacga gcccgcgcgg gagccaggtc cagatctcgg cgcggcgggg   8100 

gcggagagcg aagacgaggg cgcgcagttg ggagctgtcc atggtgtcgc ggagatccag   8160 

gtccgggggc agggttctga ggttgacctc gtagaggcgg gtgagggcgt gcttgagatg   8220 

cagatggtac ttgatctcca cgggtgagtt ggtggctgtg tccacgcatt gcatgagccc   8280 

gtagctgcgc ggggccacga ccgtgccgcg gtgcgctttt agaagcggtg tcgcggacgc   8340 

gctcccggcg gcagcggcgg ttccggcccc gcgggcaggg gcggcagagg cacgtcggcg   8400 

tggcgctcgg gcaggtcccg gtgctgcgcc ctgagagcgc tggcgtgcgc gacgacgcgg   8460 

cggttgacat cctggatctg ccgcctctgc gtgaagacca ccggccccgt gactttgaac   8520 

ctgaaagaca gttcaacaga atcaatctcg gcgtcattga cggcggcctg acgcaggatc   8580 

tcttgcacgt cgcccgagtt gtcctggtag gcgatctcgg acatgaactg ctcgatctcc   8640 

tcctcctgga gatcgccgcg gcccgcgcgc tccacggtgg cggcgaggtc attggagatg   8700 

cgacccatga gctgcgagaa ggcgcccagg ccgctctcat tccagacgcg gctgtagacc   8760 

acgtccccgt cggcgtcgcg cgcgcgcatg accacctgcg cgaggttgag ctccacgtgc   8820 

cgcgtgaaga cggcgtagtt gcgcaggcgc tggaagaggt agtttagggt ggtggcgatg   8880 

tgctcggtga cgaagaagta catgatccag cggcgcaggg gcatctcgct gatgtcgccg   8940 

atggcctcca gcctttccat ggcctcgtag aaatccacag cgaagttgaa aaactgggcg   9000 

ttgcgggccg agaccgtgag ctcgtcctcc aggagcctga tgagttcggc gatggtggcg   9060 

cgcacctcgc gctcgaaatc cccgggggcc tcctcctctt cctcttcttc catgacgacc   9120 

tcttcttcta tttcttcctc tgggggcggt ggtggtggcg gggcccgacg acgacggcga   9180 

cgcaccggga gacggtcgac gaagcgctcg atcatctccc cgcggcggcg acgcatggtt   9240 

tcggtgacgg cgcgaccccg ttcgcgagga cgcagcgtga agacgccgcc ggtcatctcc   9300 

cggtaatggg gcgggtcccc gttgggcagc gagagggcgc tgacgatgca tcttatcaat   9360 

tgcggtgtag gggacgtgag cgcgtcgaga tcgaccggat cggagaatct ttcgaggaaa   9420 

gcgtctagcc aatcgcagtc gcaaggtaag ctcaaacacg tagcagccct gtggacgctg   9480 

ttagaattgc ggttgctgat gatgtaattg aagtaggcgt ttttaaggcg gcggatggtg   9540 

gcgaggagga ccaggtcctt gggtcccgct tgctggatgc gaagccgctc ggccatgccc   9600 

caggcctggc cctgacaccg gctcaggttc ttgtagtagt catgcatgag cctctcaatg   9660 

tcatcactgg cggaggcgga gtcttccatg cgggtgaccc cgacgcccct gagcggctgc   9720 

acgagcgcca ggtcggcgac gacgcgctcg gcgaggatgg cctgttgcac gcgggtgagg   9780 

gtgtcctgga agtcgtccat gtcgacgaag cggtggtagg ccccggtgtt gatggtgtag   9840 

gtgcagttgg ccatgagcga ccagttgacg gtctgcaggc cgggttgcac gacctctgag   9900 

tacctgagcc gcgagaaggc gcgcgagtcg aagacatagt cgttgcaggt gcgcacgagg   9960 

tactggtatc caactaggaa gtgcggcggc ggctggcggt agagcggcca gcgctgggtg  10020 

gccggcgcgc ccggggccag gtcctcgagc atgaggcggt ggtagccgta gaggtagcgg  10080 

gacatccagg tgatgccggc ggcggtggtg gaggcgcgcg ggaactcgcg gacgcggttc  10140 

cagatgttgc gcagcggcag gaaatagtcc atggtcggca cggtctggcc ggtgagacgc  10200 

gcgcagtcat tgacgctcta gaggcaaaaa cgaaagcggt tgagcgggct cttcctccgt  10260 

agcctggcgg aacgcaaacg ggttaggccg cgtgtgtacc ccggttcgag tcccctcgaa  10320 

tcaggctgga gccgcgacta acgtggtatt ggcactcccg tctcgacccg agcccgatag  10380 

ccgccaggat acggcggaga gccctttttg ccgaccgagg ggagtcgcta gacttgaaag  10440 

cggccgaaaa ccccgccggg tagtggctcg cgcccgtagt ctggagaagc tttgccaggg  10500 

ttgagtcgcg gcagaacccg gttcgcggac ggccgcggcg agcgggactt ggtcaccccg  10560 

ccgatttaaa gacccacagc cagccgactt ctccagttac gggagcgagc cccctttttt  10620 

ctttttgcca gatgcatccc gtcctgcgcc aaatgcgtcc caccccccct ccggcgacca  10680 

ccgcgaccgc ggccgtagca ggcgccggcg ctgtagcccc gccacagcag acagagatgg  10740 

acttggaaga gggcgaaggg ctggcgagac tgggggcgcc gtccccggag cgacaccccc  10800 

gcgtgcagct gcagaaggac gtgcgcccgg cgtacgtgcc tgcgcagaac ctgttcaggg  10860 

accgcagcgg ggaggagccc gaggagatgc gcgactgccg ttttcgggcg ggcagggagc  10920 

tgcgcgaggg cctggaccgc cagcgcgtgc tgcgcgacga ggatttcgag ccgaacgagc  10980 

agacggggat cagccccgcg cgcgcgcacg tggcggcggc caacctggtg acggcctacg  11040 

agcagacggt gaagcaggag cgcaacttcc aaaagagttt caacaaccat gtgcgcacgc  11100 

taatcgcgcg cgaggaggtg gccctgggct tgatgcacct gtgggacctg gcggaggcca  11160 

tcgtgcagaa cccggacagc aagcctctga cggcgcagct gttcctggtg gtgcagcaca  11220 

gcagggacaa cgaggcgttc agggaggcgc tgctaaacat cgccgagccc gagggccgct  11280 

ggctgctgga gctgatcaac atcttgcaga gcatcgtagt gcaggagcgc agcctgagcc  11340 

tggccgagaa ggtggcggct atcaactact cggtgctgag cctgggcaag ttttacgcgc  11400 

gcaagattta caagacgccg tacgtgccca tagacaagga ggtgaagata gacagctttt  11460 

acatgcgcat ggcgctcaag gtgctgacgc tgagcgacga cctgggcgtg taccgcaacg  11520 

accgcatcca caaggccgtg agcgcgagcc ggcggcgcga gctgagcgac cgcgagctga  11580 

tgctgagtct gcgccgggcg ctggtagggg gcgccgccgg cggtgaggag tcctacttcg  11640 

acatgggggc ggacctgcat tggcagccga gccggcgcgc cttggaggcc gcctacggtc  11700 

cagaggactt ggatgaggat gaggaagagg aggaggatgc acccgctgcg gggtactgac  11760 

gcctccgtga tgtgttttta gatgcagcaa gccccggacc ccgccataag ggcggcgctg  11820 

caaagccagc cgtccggtct agcatcggac gactgggagg ccgcgatgca acgcatcatg  11880 

gccctgacga cccgcaaccc cgagtccttt agacaacagc cgcaggccaa cagactctcg  11940 

gccattctgg aggcggtggt cccctctcgg accaacccca cgcacgagaa ggtgctggcg  12000 

atcgtgaacg cgctggcgga gaacaaggcc atccgtcccg acgaggccgg gctggtgtac  12060 

aacgccctgc tggagcgcgt gggccgctac aacagcacaa acgtgcagtc caacctggac  12120 

cggctggtga cggacgtgcg cgaggccgtg gcgcagcgcg agcggttcaa gaacgagggc  12180 

ctgggctcgt tggtggcgct gaacgccttc ctggcgacgc agccggcgaa cgtgccgcgc  12240 

gggcaggacg attacaccaa ctttatcagc gcgctgcggc tgatggtgac cgaggtgccc  12300 

cagagcgagg tgtaccagtc gggcccagac tactttttcc agacgagccg gcagggcttg  12360 

cagacggtga acctaagcca ggctttcaag aatctgcgcg ggctgtgggg cgtgcaggcg  12420 

cccgtgggcg accggtcgac ggtgagcagc ttgctaacgc ccaactcgcg gctgctgctg  12480 

ctgctgatcg cgcccttcac cgacagcggc agcgtgaacc gcaactcgta cctgggccac  12540 

ctgctgacgc tttaccgcga ggccataggc caggcgcagg tggacgagca gaccttccag  12600 

gagatcacta gcgtgagccg cgcgctgggt cagaacgaca ccgacagtct gagagccacc  12660 

ctgaacttct tgctgacaaa tagacagcag aagattccgg cgcagtacgc gctgtcggcc  12720 

gaggaggagc gcatcctgag atatgtgcag cagagcgtag ggcttttcct gatgcaggag  12780 

ggggccaccc ccagcgccgc gctggacatg accgcgcgca acatggaacc tagcatgtac  12840 

gccgccaacc ggccgttcat caataagctg atggactacc tgcaccgcgc ggctgccatg  12900 

aactcggact actttactaa tgctatacta aacccgcact ggctcccgcc gccggggttc  12960 

tacacgggcg agtacgacat gcccgacccc aacgatgggt tcctgtggga cgacgtggac  13020 

agcgcggtgt tctccccgac cttgcaaaag cgccaggagg cggtacgcac gcccgcgagc  13080 

gagggcgcgg tgggtcggag cccctttcct agcttaggga gtttgcatag cttgccgggc  13140 

tcggtgaaca gcggcagggt gagccggccg cgcttgctgg gcgaggacga gtacctgaac  13200 

gactcgctgc tgcagccgcc gcgggtcaag aacgccatgg ccaataacgg gatagagagt  13260 

ctggtggaca aactgaaccg ctggaagacc tacgctcagg accataggga tgcgcccgcg  13320 

ccgcggcgac agcgccacga ccggcagcgg ggcctggtgt gggacgacga ggactcggcc  13380 

gacgatagca gcgtgttgga cttgggcggg agcggtgggg ccaacccgtt cgcgcatctg  13440 

cagcccagac tggggcgacg gatgttttga atgaaataaa actcaccaag gccatagcgt  13500 

gcgttctctt ccttgttaga gatgaggcgc gcggtggtgt cttcctctcc tcctccctcg  13560 

tacgagagcg tgatggcgca ggcaaccctg gaggttccgt ttgtgcctcc gcggtatatg  13620 

gctcctacgg agggcagaaa cagcattcgt tactcggaac tggctccgca gtacgacacc  13680 

actcgcgtgt acttggtgga caacaagtcg gcggacatcg cttccctgaa ctaccaaaac  13740 

gaccacagca acttcctgac cacggtggtg cagaacaacg atttcacccc cgccgaggcc  13800 

agcacgcaga cgataaattt tgacgagcgg tcgcggtggg gcggtgattt gaagaccatt  13860 

ctgcacacca acatgcccaa tgtgaacgag tacatgttca ccagcaagtt taaggcgcgg  13920 

gtgatggtgg ctaggaaggt ggtagatcag aatgatagga gcaaggatga gttaaaatat  13980 

gagtggtttg agtttaccct gcccgagggc aacttttccg agaccatgac catagacctg  14040 

atgaacaacg ccatcttgga aaactacttg caagtggggc ggcaaaatgg cgtgctggag  14100 

agcgatatcg gagtcaagtt tgacagcagg aatttcaagc tgggctggga cccggtaacc  14160 

aagctggtga tgcctggggt ctacacctac gaggccttcc acccggacgt tgtgctgctg  14220 

ccgggctgcg gggtggactt caccgagagc cgcctgagca acctcctggg cattcgcaag  14280 

aagcaacctt tccaagaggg cttcaggatc atgtatgagg atctcgaggg tggtaacatc  14340 

cccgccctcc tggatgtcaa gcaatatttg gatagtaaaa agaagcttga ggaggcaaca  14400 

cagaatgcaa ccagggctgc tggagatatc agaggagaca gtcatattcc aagagctgtg  14460 

gaacaagcgg ctgaaaagga tctggtcatt gtaccagtaa cacaagatga aagtaagaga  14520 

agctataatg tcatagatgg cacccatgac accctctacc gaagttggta cctgtcctat  14580 

acctacgggg accccgagaa gggggtgcag tcgtggacgc tgctcaccac cccggacgtc  14640 

acctgcggcg cggagcaagt ctactggtcg ctgccggacc tcatgcaaga ccccgtcacc  14700 

ttccgctcta cccagcaagt cagcaactac cccgtggttg gcgccgagct catgcccttc  14760 

cgcgccaaga gcttttacaa cgacctcgcc gtctactccc agctcatccg cagctacacc  14820 

tccctcaccc acgtcttcaa ccgcttcccc gacaaccaga tcctctgccg tccgcccgcg  14880 

cccaccatca ccacggtcag tgaaaacgtg cctgctctca cagatcacgg gacgctaccg  14940 

ctgcgcagca gtatccgcgg agtccagcga gtgaccgtca ctgacgcccg tcgccgcacc  15000 

tgtccctacg tctacaaggc cctgggcata gtcgcgccgc gcgtgctttc cagtcgcacc  15060 

ttctaaaaaa tgtctattct catctcgccc agcaataaca ccggctgggg tcttactagg  15120 

cccagcacca tgtacggagg agccaagaag cgctcccagc agcaccccgt ccgcgtccgc  15180 

ggccacttcc gcgctccctg gggcgcttac aagcgcgggc ggacttctac cgccgccgtg  15240 

cgcaccaccg tcgacgacgt catcgactcg gtggtcgccg acgcgcgcaa ctataccccc  15300 

gccccctcca ccgtggacgc ggtcatcgac agcgtggtgg ccgacgcgcg cgactatgcc  15360 

agacgcaaga gccggcggcg acggatcgcc aggcgccacc ggagtacgcc cgccatgcgc  15420 

gccgcccggg ctctgctgcg ccgcgccaga cgcacgggcc gccgggccat gatgcgagcc  15480 

gcgcgccgcg ccgccactgc accccccgca ggcaggactc gcagacgagc ggccgccgcc  15540 

gctgccgcgg ccatttctag catgaccaga cccaggcgcg gaaacgtgta ctgggtgcgc  15600 

gactccgtca cgggcgtgcg cgtgcccgtg cgcacccgtc ctcctcgtcc ctgatctaat  15660 

gcttgtgtcc tcccccgcaa gcgacgatgt caaagcgcaa aatcaaggag gagatgctcc  15720 

aggtcgtcgc cccggagatt tacggaccac cccaggcgga ccagaaaccc cgcaaaatca  15780 

agcgggttaa aaaaaaggat gaggtggacg agggggcagt agagtttgtg cgcgagttcg  15840 

ctccgcggcg gcgcgtaaat tggaaggggc gcagggtgca gcgcgtgttg cggcccggca  15900 

cggcggtggt gtttacgccc ggcgagcggt cctcggtcag gagcaagcgt agctatgacg  15960 

aggtgtacgg cgacgacgac atcctggacc aggcggcgga gcgggcgggc gagttcgcct  16020 

acgggaagcg gtcgcgcgaa gaggagctga tctcgttgcc gctggacgag agcaacccca  16080 

cgcctagcct gaagcccgtg accctgcagc aggtgctgcc ccaagcagtg ctgctgccga  16140 

gccgcggggt caagcgcgag ggcgagaata tgtacccgac catgcagatc atggtgccca  16200 

agcgccggcg cgtggaagaa gtgctggaca ccgtgaaaat ggatgtggag cccgaggtca  16260 

aggtgcgccc catcaagcag gtggcgccgg gcctgggcgt gcagaccgtg gacattcaga  16320 

tccccaccga catggatgtt gacaaaaaac cctcgaccag catcgaggtg cagaccgacc  16380 

cctggctccc agcctccacc gctgccgtct ccacttctac cgccgccacg gctaccgagc  16440 

ctcccagaag gcgaagatgg ggccctgcca accggctgat gcccaactac gtattgcatc  16500 

cttccattat cccgacgccg ggctatcgcg gcacccggta ctacgccagc cgcaggcgcc  16560 

cagccagcaa acgccgccgc cgcaccgcca cccgccgccg tctggccccc gcccgcgtgc  16620 

gccgcgtaac cacgcgccgg ggccgctcgc tcgttctgcc caccgtgcgc taccacccca  16680 

gcatccttta atccgtgtgc tgtgatactg ttgcagagag atggctctca cttgccgcct  16740 

gcgcatcccc gtcccgaatt accgaggaag atcccgccgc aggagaggca tggcaggcag  16800 

cggcctcaac cgccgccggc ggcgggccat gcgcaggcgc ctgagtggcg gctttctgcc  16860 

cgcgctcatc cccataatcg cggcggccat cggcacgatc ccgggcatag cttccgttgc  16920 

gctgcaggcg tcgcagcgcc gttgatgtgc gaataaagcc tctttagact ctgacacacc  16980 

tggtcctgta tatttttaga atggaagaca tcaattttgc gtccctggct ccgcggcacg  17040 

gcacgcggcc gttcatgggc acctggaacg agatcggcac cagccagctg aacgggggcg  17100 

ccttcaattg gagcagtgtc tggagcgggc ttaaaaattt cggctcgacg ctccggacct  17160 

atgggaacaa ggcctggaat agtagcacgg ggcagttgtt aagggaaaag ctcaaagacc  17220 

agaacttcca gcagaaggtg gtggacggcc tagcctcggg cattaacggg gtggtggaca  17280 

tagcaaacca ggccgtgcag cgcgagataa acagccgcct ggacccgcgg ccgcccacgg  17340 

tggtggagat ggaagatgca actcctccgc cgcccaaggg cgagaagcgg ccgcggcccg  17400 

acgcggagga gacgatcctg caggtggacg agccgccctc gtacgaggag gccgtcaagg  17460 

ccggcatgcc caccacgcgt atcatcgcgc cactggccac tggtgtaatg aaacccgcca  17520 

cccttgacct gcctccgcca cccacgcccg ctccaccgaa ggcagctccg gttgtgcagc  17580 

cccctcctgt ggcgaccgcc gtgcgccgcg tccccgcccg ccgccaggcc cagaactggc  17640 

agagcacgct gcacagtatc gtgggcctgg gagtgaaaag tctgaagcgc cgccgatgct  17700 

attgagagag aggaaagagg acactaaagg gagagcttaa cttgtatgtg ccttaccgcc  17760 

agagaacgcg cgaagatggc taccccctcg atgatgccgc agtgggcgta catgcacatc  17820 

gccgggcagg acgcctcgga gtacctgagc ccgggtctgg tgcagtttgc ccgcgccacc  17880 

gacacgtact tcagcctggg caacaagttt aggaacccca cggtggctcc cacccacgat  17940 

gtgaccacgg accggtccca gcgtctgacg ctgcgctttg tgcccgtgga tcgcgaggac  18000 

accacgtact cgtacaaggc gcgcttcact ctggccgtgg gcgacaaccg ggtgctagac  18060 

atggccagca cttactttga catccgcggc gtcctggacc gcggtcccag cttcaaaccc  18120 

tactcgggca cggcttacaa cagcctggcc cccaaaggcg cccccaactc tagtcagtgg  18180 

gaacaagcta aagctaccaa tgccggtcaa aaggaaactc acacatttgg agtagccgct  18240 

atgggcggag aagacattac agtgaaaggt cttcaaattg gaactgatga aactaaggaa  18300 

gatggagagg atgaaatttt tgcagatcaa acattccagc cagaacctca agtgggagaa  18360 

cagaactggc aagaaacgtt tgttttctat ggaggcagag ctcttaagaa agaaaccaaa  18420 

atgaagccat gttatggctc ttatgcgaga cccacaaatg aaaagggagg acaggctaaa  18480 

tttacacttg atgaaaaagg tcagccaacc aaaattcctg atattacaat ggatttcttt  18540 

gatagtccac aagatgatac atcaggtgta actaataagc cagatattgt catgtatgca  18600 

gaaaatgtaa atttagaagc tcctgacaca catgtagttt acaaaccagg caaagatgat  18660 

tctagttctt ccgctaacct cacacaacag gccatgccta acagaccgaa ctacatcggg  18720 

ttcagagaca actttgtggg tcttatgtac tacaatagta ctggcaacat gggtgtgctg  18780 

gctggtcagg cctctcagtt gaatgctgtg gtcgacttgc aagacagaaa caccgagctg  18840 

tcttaccagc tattgctaga ttctctgggt gacagaacca gatactttag catgtggaat  18900 

tctgcagtgg acagctatga ccccgatgtc aggatcattg agaatcacgg tgtggaagat  18960 

gaacttccaa actattgctt cccactgaat ggcagtggtt ctaacagcac atacaaaggt  19020 

gttaaagctg gaactggaaa caattgggat gacgatgaaa atgttgcaag acaaaatcag  19080 

attggcactg gcaacctgtt cgccatggag atcaacctcc aggccaacct atggaagagt  19140 

tttctgtact cgaacgtggc cctgtacctg cccgactcct acaagtacac gccggccaac  19200 

gtcacgctgc ccaccaacac caacacctac gactacatga acggccgcgt ggtagccccc  19260 

tcgctggtgg acgcctacat caacattggc gcccgctggt cgctggaccc catggacaat  19320 

gtcaatccct tcaaccacca ccgcaacgcg ggcctgcgct accgctccat gctcctgggc  19380 

aacggccgct acgtgccctt ccacatccaa gtgccccaaa agttctttgc catcaagaac  19440 

ctgcttctgc tccccggttc ctacacctac gagtggaact tccgcaagga cgtcaacatg  19500 

atcctgcaga gttccctcgg caacgacctg cgcgtcgacg gcgcctccgt ccgcttcgac  19560 

agcgtcaacc tctacgccac cttcttcccc atggcgcaca acaccgcctc caccctggaa  19620 

gccatgctgc gcaacgacac caacgaccag tccttcaacg actacctctc ggccgccaac  19680 

atgctctacc ccatcccggc caaggccacc aacgtgccca tctccatccc ctcgcgcaac  19740 

tgggccgcct tccgcggctg gagtttcacc cggctcaaga ccaaggaaac tccctccctc  19800 

ggctcgggtt tcgaccccta ctttgtctac tcgggctcca tcccctacct cgacgggacc  19860 

ttctacctca accacacctt caagaaggtc tccatcatgt tcgactcctc ggtcagctgg  19920 

cccggcaacg accggctgct cacgccgaac gagttcgaga tcaagcgcag cgttgacggg  19980 

gagggctaca acgtggccca atgcaacatg accaaggact ggttcctcgt ccagatgctc  20040 

tcccactaca acatcggcta ccagggcttc cacgtgcccg agggctacaa ggaccgcatg  20100 

tactccttct tccgcaactt ccagcccatg agcaggcagg tggtcgatga gatcaactac  20160 

aaggactaca aggccgtcac cctacccttc cagcacaaca actcgggctt caccggctac  20220 

cttgcgccca ccatgcgcca ggggcagccc taccccgcca acttccccta cccgctcatc  20280 

ggctccaccg cagttccctc cgtcacccag aaaaagttcc tctgcgacag ggtcatgtgg  20340 

cgcatcccat tctccagcaa ctttatgtcc atgggcgccc tcaccgacct gggtcagaac  20400 

atgctctatg ccaactcggc ccacgcgctc gacatgacct ttgaggtgga ccccatggat  20460 

gagcccaccc tcctctatct tctcttcgaa gttttcgacg tggtcagagt gcaccagccg  20520 

caccgcggcg tcatcgaggc cgtctacctg cgcacgccct tctccgccgg caacgctacc  20580 

acttaagcat gagcggctcc agcgaacaag agctcgcggc catcgtgcgc gacctgggat  20640 

gcgggcccta ctttttggga acccacgaca agcgcttccc tggcttcctt gccggcgaca  20700 

agctggcctg cgccatcgtc aacacggccg gccgcgagac cggaggcgtg cactggctcg  20760 

cctttggctg gaatccgcgc tcgcgcacct gctacatgtt cgaccccttt gggttctcgg  20820 

accgccggct caagcagatt tacagcttcg agtacgaggc catgctgcgc cgaagcgcgc  20880 

ttgcctcctc gcccgaccgc tgtctcagcc tcgagcagtc cacccagacc gtgcaggggc  20940 

ccgactccgc cgcctgcgga cttttttgtt gcatgttttt gcatgccttc gtgcactggc  21000 

ccgaccgacc catggacgga aaccccacca tgaacttgct gacgggggtg ccaaacggca  21060 

tgctacaatc gccacaggtg ctgcccaccc tcaggcgcaa ccaggaggag ctctaccgct  21120 

tcctcgcgcg ccactcccct tactttcgat cccaccgcgc cgccatcgaa aacgccaccg  21180 

cttttgataa aatgaaacaa ctgcgtgtat ctcaataaac agcactttat tttacatgca  21240 

ctggagtata tgcaagttat ttaaaagtcg aaggggttct cgcgctcgtc gttgtgcgcc  21300 

gcgctgggga gggccacgtt gcggtactgg tacttgggaa gccacttgaa ctcggggatc  21360 

accagtttgg gcactggggt ctcggggaag gtctcgctcc acatgcgccg gctcatctgc  21420 

agggcgccca gcatgtccgg gccggagatc ttgaaatcac aattggggcc ggtgctctgc  21480 

gcgcgcgagt tgcggtacac ggggttgcag cactggaaca ccattagact ggggtacttc  21540 

acactggcaa gcacgctctt gtcgctgatc tgatccttgt ccaggtcctc ggcgttgctc  21600 

aggccgaacg gggtcatctt gcacagctgg cggcccagga agggcacgct ctgaggcttg  21660 

tggttacact cgcagtgcac gggcatcagc atcatccccg cgccgcgctg catattcggg  21720 

tagagggcct tgacgaaggc cgtgatctgc ttgaaagctt gctgggcctt agccccctcg  21780 

ctgaaaaaca ggccgcagct cttcccgcta aactggttat tcccgcaccc ggcatcatgc  21840 

acgcagcagc gcgcgtcatg gctggtcagt tgcaccacgc tacgtcccca gcggttctgg  21900 

gtcaccttgg ccttgctggg ctgctccttc aacgcgcgct gcccgttctc gctggtcaca  21960 

tccatctcca ccacgtggtc cttgtggatc atcaccgtcc catgcagaca cttgagctga  22020 

ccctcgacat cgcagcagcc atgatcccac agggcgcagc cggtgcactc ccagttctta  22080 

tgcgcgatcc cgctgtggct gaagatgtaa ccttgcaaca ggcgacccat gacggtgcta  22140 

aatgctttct gggtggtgaa ggtcagttgc agaccgcggg cctcctcgtt catccaggtc  22200 

tggcacatct tttggaagat ctcggtctgc tcgggcatga gcttgtaagc atcgcgcagg  22260 

ccgctgtcga cgcggtagcg ttccatcagc acgttcatgg tatccatgcc cttctcccag  22320 

gacgagacca gaggcagact cagggggttg cgcacgttca ggacaccggg ggtcgcaggc  22380 

tcgacgatgc gttttccgtc cttgccttcc ttcaacagaa ccggaggctg gctgaatccc  22440 

actcccacga ttacggcatc ttcctggggc atctcttcgt cggggtctac cttggtcaca  22500 

tgcttggtct ttctggcttg cttctttttt ggagggctgt ccacggggac cacgtcctcc  22560 

tcggaagacc cggagcccac ccgctgatac tttcggcgct tggtgggcag aggaggtggt  22620 

ggcggcgagg ggctcctctc ctgctccggc ggatagcgcg ccgacccgtg gccccggggc  22680 

ggagtggcct ctcgctccat gaaccggcgc acgtcctgac tgccgccggc cattgtttcc  22740 

taggggaaga tggaggagca gccgcgtaag caggagcagg aggaggactt aaccacccac  22800 

gagcaaccca aaatcgagca ggacctgggc ttcgaagagc cggctcgtct agaaccccca  22860 

caggatgaac aggagcacga gcaagacgca ggccaggagg agaccgacgc tgggctccag  22920 

catggctacc tgggaggaga ggaggatgtg ctgctaaaac acttgcagcg ccaatccatc  22980 

atcctccggg acgccctggc cgaccggagc gaaacccctc tcagcgtcga ggagctgtgt  23040 

cgggcctacg agctcaacct cttctcgccg cgcgtgcccc ccaaacgcca gcccaacggc  23100 

acctgcgagc ccaacccgcg tctcaacttc tatcccgtct ttgcggtccc cgaggcccta  23160 

gccacctatc acatcttttt caagaaccaa aagatccccg tctcctgccg cgccaaccgc  23220 

acccgcgccg acgcgctcct cgctctgggg cccggcgcgc gcatacctga tatcgcttcc  23280 

ctggaagagg tgcccaagat cttcgaaggg ctcggtcggg acgagacgcg cgcggcaaac  23340 

gctctgaaag aaacagcaga ggaagagggt cacactagcg ccctggtaga gttggaaggc  23400 

gacaacgcca ggctggccgt gctcaagcgc agcgtcgagc tcacccactt cgcctacccc  23460 

gccgtcaacc tcccgcccaa ggtcatgcgt cgcatcatgg atcagctcat catgccccac  23520 

atcgaggccc tcgatgaaag tcaggagcag cgccccgagg acgcccggcc cgtggtcagc  23580 

gacgagcagc tcgcgcgttg gctcgggacc cgcgaccccc aggctttgga acagcggcgc  23640 

aagctcatgc tggccgtggt cctggtcacc ctcgagctcg aatgcatgcg ccgcttcttc  23700 

agcgaccccg agaccctgcg taaggtcgag gagaccctgc actacacttt caggcacggt  23760 

ttcgtcaggc aggcctgcaa gatctccaac gtggagctga ccaacctggt ctcatgcctg  23820 

gggatcctgc acgagaaccg cctgggacag accgtgctcc actctactct gaagggcgag  23880 

gcgcgtcggg actatgtccg cgactgtgta tttctcttta tctgccacac ctggcaagca  23940 

gccatgggcg tgtggcagca gtgtctcgag gacgaaaatc tgaaggagct ggacaagctt  24000 

cttgctagaa accttaaaaa gctgtggacg ggcttcgacg agcgcaccgt cgcctcggac  24060 

ctggccgaga tcgtttttcc agaacgcctg aggcagacgc tgaaaggcgg gctgcccgac  24120 

ttcatgagcc agagcatgtt gcaaaactac cgcactttca ttctcgagcg atctgggatg  24180 

ctacccgcca cctgcaacgc attcccctcc gactttgtcc cgctgagcta ccgcgagtgt  24240 

cccccgccgc tgtggagcca ctgctatctc ttgcagctgg ccaactacat cgcctaccac  24300 

tcggacgtga tcgaggacgt gagcggcgag gggcttctcg agtgccactg ccgctgcaac  24360 

ctgtgctccc cgcaccgctc cctggtctgc aacccccagc ttctgagcga gacccaggtc  24420 

atcggtacct tcgagctgca aggtccgcag gagtccaccg ctccgctgaa actcacgccg  24480 

gggttgtgga cttccgcgta cctgcgcaaa tttgtacccg aggactacca cgcccatgaa  24540 

ataaagttct tcgaggacca atcgcgccca cagcacgcgg atctcacggc ctgcgtcatc  24600 

acccagggcg cgatcctcgc ccaattgcac gccatccaaa aatcccgcca agagtttctt  24660 

ctaaaaaagg gtagaggggt ctacctggac ccccagacgg gcgaggtgct caacccgggt  24720 

ctcccccagc atgccgagga agaagcagga gccgctagtg gagcagatgg aagaagaatg  24780 

ggacagccag gcagaggagg acgaatggga ggaggagaca gaggaggaag aattggaaga  24840 

ggtggaagag gagcaggaaa cagagcagcc cgtcgccgca ccatccgcgc cggcagcccc  24900 

gccggtcacg gatacaacct ccacagctcc ggccaagcct cctcgtagat gggatcgagt  24960 

gaagggtgac ggtaagcacg agcggcaggg ctaccgatca tggagggtcc acaaagccgc  25020 

gatcatcgcc tgcttgcaag actgcggggg gaacatcgct ttcgcccgcc gctacctgct  25080 

cttccaccgc ggggtgaaca tcccccgcaa cgtgttgcat tactaccgtc accttcacag  25140 

ctaagaaaaa gcaagtaaga ggagtcgccg gaggaggcct gaggatcgcg gcgaacgagc  25200 

cctcgaccac cagggagctg aggaaccgga tcttccccac tctttatgcc atttttcagc  25260 

agagtcgagg tcagcagcaa gaactgaaag taaaaaaccg gtctctgcgc tcgctcaccc  25320 

gcagttgctt gtaccacaaa aacgaagatc agctgcagcg cactctcgaa gacgccgagg  25380 

ctctgttcca caagtactgc gcgctcactc ttaaagacta aggcgcgccc acccggaaaa  25440 

aaggcgggaa ttacctcatc gccaccatga gcaaggagat tcccacccct tacatgtgga  25500 

gctatcagcc ccagatgggc ctggccgcgg gcgcctccca ggactactcc acccgcatga  25560 

actggctcag tgccggcccc tcgatgatct cacgggtcaa cggggtccgt aaccatcgaa  25620 

accagatatt gttggagcag gcggcggtca cctccacgcc cagggcaaag ctcaacccgc  25680 

gtaattggcc ctccaccctg gtgtatcagg aaatccccgg gccgactacc gtactacttc  25740 

cgcgtgacgc actggccgaa gtccgcatga ctaactcagg tgtccagctg gccggcggcg  25800 

cttcccggtg cccgctccgc ccacaatcgg gtataaaaac cctggtgatc cgaggcagag  25860 

gcacacagct caacgacgag ttggtgagct cttcgatcgg tctgcgaccg gacggagtgt  25920 

tccaactagc cggagccggg agatcgtcct tcactcccaa ccaggcctac ctgaccttgc  25980 

agagcagctc ttcggagcct cgctccggag gcatcggaac cctccagttc gtggaggagt  26040 

ttgtgccctc ggtctacttc aaccccttct cgggatcgcc aggcctctac ccggacgagt  26100 

ttataccgaa cttcgacgca gtgagagaag cggtggacgg ctacgactga atgtcccatg  26160 

gtgactcggc tgagctcgct cggttgaggc atctggacca ctgccgccgc ctgcgctgct  26220 

tcgcccggga gagctgcgga ctcatctact ttgagtttcc cgaggagcac cccaacggcc  26280 

ctgcacacgg agtgcggatc accgtagagg gcaccaccga gtctcacctg gtcaggttct  26340 

tcacccagca acccttcctg gtcgagcggg accggggagc taccacctac accgtctact  26400 

gcatctgtcc taccccgaag ttgcatgaga atttttgctg tactctttgt ggtgagttta  26460 

ataaaagctg aactaagaac cttctttgga atcccttgtc atcatcaaat caacaagacc  26520 

atcaacttca cctttgagga acaggtgaac tttacctgca agccacacaa gaagtacatc  26580 

atctggtttt atcacaacac tactctagca gtagccaaca cctgctcgaa cgacggtgtt  26640 

ctcctaccta acaatctcac cagtggacta accttctcag ttaaaagggc aaagctaatt  26700 

cttcatcgcc ctattgtaga aggaacttac cagtgtcaga gcggaccttg cttccacagt  26760 

ttcactttgg tgaacgttac cggcagcagc acagccgctc cagaaacatc taaccttctt  26820 

tctgatacta acaaacctcg tgtcggaggt gagctttggg ttccatctct aacagagggt  26880 

gggagttcta ttgaagtggt tgggtatttg attttagggg tggtcattgg tgggtgcata  26940 

gcagtgctgt atcaacttcc ttgctgggtc gaaatcaggg tatttatctg ctgggtcaga  27000 

cattgtgggg aggaaccatg aaggggctct tgctgattat cctttccctg gtggggggtg  27060 

tgctgtcatg ccacgaacag ccacgatgta acattaccac aggcaatgag aggaacgact  27120 

gctctgtagt tatcaaatgc gagcaccatt gtcctctcaa catcacattc aagaataaga  27180 

ccatgggaaa tgtatgggtg ggattctggc aaccaggaga tgagcagaac tacacggtca  27240 

ctgtccatgg tagcgatggc aatcacactt tcggtttcaa attcattttt gaagtcatgt  27300 

gtgatatcac actacatgtg gctagacttc atggcttgtg gccccctacc aaggagaaca  27360 

tggtgggttt ttctttggct tttgtgatca tggcctgctt gatgtcaggt ctgctggtag  27420 

gggctctagt gtggtttctg aaacgcaagc ccaggtacgg aaatgaggag aaggaaaaat  27480 

tgctataaat tctttttctc ttcgcacaac catgaataca gtgttccgta tcgtgctgct  27540 

ctctcttctt gtagctttcg gtcaggcagg aattcatatt attaatgcta catggtggga  27600 

taatataact ttagtgggac cctcagatac tccagttacc tggtatgatg gcaagggatt  27660 

gcaattttgt gacggaagta cagttaagaa tccgcagatc agacatactt gtaatgatca  27720 

aaacttaact ctgattcatg ttaacaaaac ccatgaaaga acatacatgg gttacagaca  27780 

tgacagtaag ggaaaagtag actataaggt tacagtcatt ccacctcctc ctgctactgt  27840 

aaagccacaa ccagatccag aaaatgtctt tgtttatatg ggaaataatg taactttagt  27900 

tggacctcca ggaattccag ttagttggta ttatcataat ggcacacagt tctgcgatgg  27960 

agataaaatt attcatccag aattcaacca cacctgtgat aaacaaaacc ttacactgct  28020 

gtttgtaaac tttacacatg atggaggcta tcttggattc aattacaaag gtactcagag  28080 

aattcagtat gaggttatag ttttagatcg atttccaaat tctggtcaga tgaaaattga  28140 

agaacaaagt gaggaaacag aacagaaaca tactgagcat aataaggctg gacaaaagca  28200 

gggtatagat acaaatcaaa agaaagctaa taacagacaa aagccatctc aaaggccatc  28260 

aagaagacgg ccgacaaaca ctcctgagac aaaacaactt acagtgtcta ttgggtctaa  28320 

cttaacttta gttggtccag atggaaaagt cacttggtat gatggtgatt taaaaagacc  28380 

atgtgaagaa caaaactata ggcttccaca tcagtgtagt gctcagaact taactttaat  28440 

taatgtaact aaatctcatg agggaactta ctatggcact aatgacaaag acgaaagcaa  28500 

aagatacaga gtgaaagtga acactacaaa ttctcaagct gtaaaaatta acccatatac  28560 

cagacctact actcctgatc agaaacacag atttgaatta caaattgaaa ataatgcaaa  28620 

tgatgaagaa tcaaaaattc catctactac tgtggcaatc gtggtgggag tgattgcggg  28680 

cttcataact ataatcattg tcattctgtg ctacatctgc tgccgcaagc gtcccagggc  28740 

atacaatcat atggtagacc cactactcag cttctcttac tgagactcag tcactttcat  28800 

ttcagaacca tgaaggcttt cacagcttgc gttctgttta acataatcac acttagtgta  28860 

gctgcaaatg gttttaaaca tgttaatgtt accagattaa gtaatgtaac actgacagga  28920 

gctggaatta atactacatg gacagggtat tttaatgagg gtccaaaagg aaaaaatggg  28980 

tggatgaata tttgcacatg gggcgatcct agatatgtgt gccatggaaa tagcagtact  29040 

attactaatc ttacagttgt ggcacttcta aatttaacca ctaacagaag atttaaagca  29100 

gaaagtttta ctagtaacga tggttatgaa actaccagtg caaaatttta tgaaattaaa  29160 

attattgagc ttccaacaac tagagcaccc accacagtta ggacaacaca gcctaccact  29220 

gtgcccacta cacatccaac caccacagtc agtacaacta ttgagaccac tactcatact  29280 

acacagctag acacaacagt gcagaatact actttattga ttgggttttt actgagagga  29340 

aatgaaagta ctactgaaca gacagaggct acctcaagtg ccttcagcag cactgcaaat  29400 

ttaacttcgc ttgcttggac taatgaaacc ggagtatcat tgatgaatcg acagccttac  29460 

tcaggtttgg atattcaaat tacttttctg gttgtctgtg ggatctttat tcttgcggtt  29520 

cttctgtact ttgtctgctg caaagccaga gagaaatcta ggcggcccat atacaggcca  29580 

gtaatcgggg aacctcagcc tctccaagtg gatggaggct taaggaatct tctcttctct  29640 

tttacagtat ggtgatcagc catgattcct aggttcttcc tatttaacat cctgttctgt  29700 

ctcttcaaca tctgtgctgc cttcgcggcc gtctcgcacg cctcgcccga ctgtctaggg  29760 

cctttcccaa catacctcct ctttgccctg ctaacctgca cctgcgtctg cagcattgtc  29820 

tgcgtggtca tcacctttct gcagctcatc gactggtgct gcgcgcgcta caattatctc  29880 

caccacagtc ccgaatacag ggacgagaac gtagccagaa tcttaaggct catctgacca  29940 

tgcagcctct gctcatgctg atatccctcc tatcccctgc ccttgccact tctgctgatt  30000 

actctaaatg caaattcgcg gacatatgga atttcttaga ttgctatcag gagaaaattg  30060 

atatgccctc ctattacttg gtgattgttg gggtagtcat ggtctgctca tgcactttct  30120 

ttgccattat gatctacccc tgttttaatc ttggctggaa ctctgttgag gcattcacat  30180 

acacactaga aaacagttca ctagcctcca cgccaccacc cacaccgcct ccccgcagaa  30240 

atcagttccc tatgattcag tacttagaag agccccctcc ccggccccct tccactgtta  30300 

gctactttca cataaccggc ggcgatgact gaccacctgg acctcgagat ggacggccag  30360 

gcctccgagc agcgcatcct gcaactgcgc gtccgacagc agcaggagcg ggccgccaag  30420 

gagctcctcg atgccatcaa catccaccag tgcaagaagg gcatcttctg cctggtcaag  30480 

caggcaaaga tcacctacga gctcgtgtcc ggcggcaagc agcatcgcct cgcctatgag  30540 

ctaccccagc agaagcaaaa gttcacctgc atggtgggcg tcaaccccat agtcatcacc  30600 

cagcagtcgg gcgagaccaa cggctgcatc cactgctcct gcgaaagccc cgagtgcatc  30660 

tactccctcc tcaagaccct ttgcggactc cgcgacctcc tccccatgaa ctgatgttga  30720 

ttaaaagccc aaaaaccaat caaacccttc cccaattact cataagaata aatcattgga  30780 

actaatcatt caataaagat cacttacttg aaatctgaaa gtatgtctct ggtgtagttg  30840 

ttcagcagca cctcggaacc ctcctcccag ctctggtact ccagtccccg gcgggcggcg  30900 

aacttcctcc acaccttgaa agggatgtca aattcctggt ccacaatttt cattgtcttc  30960 

cctcagatga caaagaggct ccgggtggaa gatgacttca accccgtcta cccctatggc  31020 

tacgcgcgga atcagaatat ccccttcctt actcccccct ttgtttcttc cgatggattc  31080 

caaaacttcc cacctggggt cctgtcactc aaactggctg acccaatcgc catcactaat  31140 

ggggatgttt cactcaaggt gggagggggt cttactgttg aaaaagatag tggaaatcta  31200 

aaggtgaacc ctaaggctcc cttgcaagtt acaactgata aacagttgga aattgcactg  31260 

gcttatccat ttgaagtcag taatggcaag cttggcataa aagcaggtca tggattgaaa  31320 

gtcattgaca aaattgctgg tttggaaggt ttggcaggta cgcttgtagt tttgactgga  31380 

aaaggaatag gtactgaaaa tcttgaaaac agtgatgggt caagtagagg agttggtata  31440 

aacgtaagac ttgctaaaga tggaggtctg tcttttgata aaaagggtga tttagttgct  31500 

tggaataaac atgatgacag acgcactcta tggacaactc ccgacccatc cccaaattgt  31560 

acaatcgatc aggaaaggga ttcaaagctc actttagtat taacaaaatg tggcagtcaa  31620 

attttggcta atgtctcttt acttgttgta aaaggaaaat ttagtaacat aaacaataat  31680 

actaatccaa ctgataaaaa aatcacagta aagctacttt ttaatgaaaa gggagtatta  31740 

atggacagtt cgacacttaa gaaagaatat tggaactaca gaaatgataa ttctactgta  31800 

tctcaggcct atgataatgc agttcctttt atgccaaaca taaaagctta tcctaaacct  31860 

accacagaca cttcggctaa accagaagat aaaaaaagtg ctgctaaaag atacattgtg  31920 

agcaatgtct atattggagg cttgccagat aaaactgttg ttataactat taagtttaat  31980 

gcagaaactg aatgtgctta ttcgattacc tttgaattca catgggcaaa aacctttgaa  32040 

gatgtgcagt ttgattcctc ctcttttacc ttttcctata ttgcccaaga aaatgaggac  32100 

gaagacaaat aaaatgtttt aaaatgaatt catgtatctt tattgatttt tacaccagca  32160 

cgggtagtca gtctcccacc accagcccat ttcacagtgt aaacgattct ctcagcacgg  32220 

gtggccttaa atagggaaat gttctgatta gtgcgggaac tggacttggg gtctataatc  32280 

cacacagttt cctggcgagc caaacggggg tcggtgattg agatgaagcc gtcctctgaa  32340 

aagtcatcca agcgggcctc acagtccaag gtcacagtct ggtgaaacga gaagaacgca  32400 

cagattcata ctcggaaaac aggatgggtc tgtgcctctc catcagcgcc ctcaacagtc  32460 

tctgccgccg gggctcggtg cggctgctgc agatgggatc gggatcacaa gtctctctga  32520 

ctatgatccc cacagccttc agcatcagtc tcctggtgcg tcgggcacag caccgcatcc  32580 

tgatctcgct catgttctca cagtaagtgc agcacataat caccatgtta ttcagcagcc  32640 

cataattcag ggtgctccag ccaaaactca tgttggggat gatggaaccc acgtgaccat  32700 

cgtaccagat gcggcagtat atcagatgcc tgcccctcat gaacacactg cccatataca  32760 

tgatctcttt gggcatgtct ctgttcacaa tctgacggta ccagggaaag cgctggttga  32820 

acatgcaccc gtaaatgact ctcctgaacc acacggccag cagggtgcct cccgcccgac  32880 

actgcaggga gcccggggat gaacagtggc aatgcaggat ccagcgctcg tacccgctca  32940 

ccatctgagc tctcaccaag tccagggtag cggggcacag gcacactgac atacatcttt  33000 

ttaaaatttt tatttcctct ggagtcaaga tcatatccca ggggactgga aactcttgga  33060 

gcagggtaaa gccagcagca catggtaatc cacggacaga acttacatta tgataatctg  33120 

catgatcaca atcaggcaac aggggatgtt gttcagtcag tgaagccctg gtttcctcat  33180 

cagatcgtgg taaacgggcc ctgcgatatg gatgatggcg gagcgagctg gattgaatct  33240 

cggtttgcat tgtagtggat tctcttgcgt accttgtcgt acttctgcca gcagaaatgg  33300 

gcccttgaac agcagatacc cctcctgcgg ccgtcctttc gctgctgccg ctcagtcatc  33360 

caactgaagt acatccattc tcgaagattc tggagaagtt cctctgcatc tgatgaaaca  33420 

aaaaacccgt ccatgcgaat tcccctcatc acatcagcca ggactctgta ggccatcccc  33480 

atccagttaa tgctgccttg tctatcattc agagggggcg gtggcaggat tggaagaacc  33540 

atttttattc caaacggtct cgaaggacga taaagtgcaa gtcacgcagg tgacagcgtt  33600 

cccctccgct gtgctggtgg aaacagacag ccaggtcaaa acccactcta ttttcaaggt  33660 

gctcgaccgt ggcttcgagc agtggctcta cgcgtacatc cagcataaga atcacattaa  33720 

aggctggccc tccatcgatt tcatcaatca tcaggttaca ttcctgcacc atccccaggt  33780 

aattctcatt tttccagcct tggattatct ctacaaattg ttggtgtaag tccactccgc  33840 

acatgtggaa aagctcccac agtgccccct ccactttcat aatcaggcag accttcataa  33900 

tagaaacaga tcctgctgct ccaccacctg cagcgtgttc aaaacaacaa gattcaataa  33960 

ggttctgccc tccgccctga gctcgcgcct caatgtcagc tgcaaaaaat cacttaagtc  34020 

ctgggccact acagctgaca attcagagcc agggctaagc gtgggactgg caagcgtaag  34080 

ggaaaacttt aatgctccaa agctagcacc caaaaactgc atgctggaat aagctctctt  34140 

tgtgtctccg gtgatgcctt ccaaaatgtg agtgataaag cgtggtagtt tttctttaat  34200 

catttgcgta atagaaaagt cctgtaaata agtcactagg accccaggga ccacaatgtg  34260 

gtagcttaca ccgcgtcgct gaagcatggt tagtagagat gagagtctga aaaacagaaa  34320 

gcatgcacta aactaaggtg gctattttca ctgaaggaaa aatcactctc tccaacaaca  34380 

gggtacccac tgggtggccc ttgcggacat acaaaaatcg gtccgtgtga ttaaaaagca  34440 

gcacagtaag ttcctgtctt cttccggcaa aaatcacatc ggactgggtt agtatgtccc  34500 

tggcatggta gtcattcaag gccataaatc tgccctgata tccagtagga accagcacac  34560 

tcacttttag gtgaagcaat accaccccat gcggaggaat gtggaaagat tcagggcaaa  34620 

aaaaattata tctattgcta gtcccttcct ggacgggagc aatccctcca ggactatcta  34680 

tgaaagcata cagagattca gccatagctc agcccgctta ccagtagaca gagagcacag  34740 

cagtacaagc gccaacagca gcgactgact acccactgac ccagctccct atttaaaggc  34800 

gccttacact gacgtaatga ccaaaggtct aaaaaccccg ccaaaaaaaa acacacacgc  34860 

cctgggtgtt ttttgcgaaa acacttccgc gttctcactt cctcgtattg atttcgtgac  34920 

ttaacttccg ggttcccacg ttacgtcact tctgccctta catgtaactc agtcgtaggg  34980 

cgccatcttg cccacgtcca aaatggcttc catgtccagc cacgcctccg cggcgaccgt  35040 

tagccgtgcg tcgtgacgtc atttgcatca tcttctctcg tccaatcagc gctggccccg  35100 

ccctaaattc aaaagctcat ttgcatgtta acttttgttt actttgtggg gtatattatt  35160 

gatgatc                                                            35167 

 
           
             2  
             49  
             DNA  
             Artificial Sequence  
             
               short synthetic polyA signal  
             
           
            2 

aataaaagat ctttattttc attagatctg tgtgttggtt ttttgtgtg                 49 

 
           
             3  
             1521  
             DNA  
             Artificial Sequence  
             
               optimized human HIV-1 gag open reading frame  
             
           
            3 

atgggtgcta gggcttctgt gctgtctggt ggtgagctgg acaagtggga gaagatcagg     60 

ctgaggcctg gtggcaagaa gaagtacaag ctaaagcaca ttgtgtgggc ctccagggag    120 

ctggagaggt ttgctgtgaa ccctggcctg ctggagacct ctgaggggtg caggcagatc    180 

ctgggccagc tccagccctc cctgcaaaca ggctctgagg agctgaggtc cctgtacaac    240 

acagtggcta ccctgtactg tgtgcaccag aagattgatg tgaaggacac caaggaggcc    300 

ctggagaaga ttgaggagga gcagaacaag tccaagaaga aggcccagca ggctgctgct    360 

ggcacaggca actccagcca ggtgtcccag aactacccca ttgtgcagaa cctccagggc    420 

cagatggtgc accaggccat ctccccccgg accctgaatg cctgggtgaa ggtggtggag    480 

gagaaggcct tctcccctga ggtgatcccc atgttctctg ccctgtctga gggtgccacc    540 

ccccaggacc tgaacaccat gctgaacaca gtggggggcc atcaggctgc catgcagatg    600 

ctgaaggaga ccatcaatga ggaggctgct gagtgggaca ggctgcatcc tgtgcacgct    660 

ggccccattg cccccggcca gatgagggag cccaggggct ctgacattgc tggcaccacc    720 

tccaccctcc aggagcagat tggctggatg accaacaacc cccccatccc tgtgggggaa    780 

atctacaaga ggtggatcat cctgggcctg aacaagattg tgaggatgta ctcccccacc    840 

tccatcctgg acatcaggca gggccccaag gagcccttca gggactatgt ggacaggttc    900 

tacaagaccc tgagggctga gcaggcctcc caggaggtga agaactggat gacagagacc    960 

ctgctggtgc agaatgccaa ccctgactgc aagaccatcc tgaaggccct gggccctgct   1020 

gccaccctgg aggagatgat gacagcctgc cagggggtgg ggggccctgg tcacaaggcc   1080 

agggtgctgg ctgaggccat gtcccaggtg accaactccg ccaccatcat gatgcagagg   1140 

ggcaacttca ggaaccagag gaagacagtg aagtgcttca actgtggcaa ggtgggccac   1200 

attgccaaga actgtagggc ccccaggaag aagggctgct ggaagtgtgg caaggagggc   1260 

caccagatga aggactgcaa tgagaggcag gccaacttcc tgggcaaaat ctggccctcc   1320 

cacaagggca ggcctggcaa cttcctccag tccaggcctg agcccacagc ccctcccgag   1380 

gagtccttca ggtttgggga ggagaagacc acccccagcc agaagcagga gcccattgac   1440 

aaggagctgt accccctggc ctccctgagg tccctgtttg gcaacgaccc ctcctcccag   1500 

taaaataaag cccgggcaga t                                             1521 

 
           
             4  
             2550  
             DNA  
             Artificial Sequence  
             
               gag expression cassette  
             
           
            4 

ccattgcata cgttgtatcc atatcataat atgtacattt atattggctc atgtccaaca     60 

ttaccgccat gttgacattg attattgact agttattaat agtaatcaat tacggggtca    120 

ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct    180 

ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta    240 

acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac    300 

ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt    360 

aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag    420 

tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat    480 

gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat    540 

gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc    600 

ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt    660 

ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga    720 

caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt    780 

gccaagagtg agatctacca tgggtgctag ggcttctgtg ctgtctggtg gtgagctgga    840 

caagtgggag aagatcaggc tgaggcctgg tggcaagaag aagtacaagc taaagcacat    900 

tgtgtgggcc tccagggagc tggagaggtt tgctgtgaac cctggcctgc tggagacctc    960 

tgaggggtgc aggcagatcc tgggccagct ccagccctcc ctgcaaacag gctctgagga   1020 

gctgaggtcc ctgtacaaca cagtggctac cctgtactgt gtgcaccaga agattgatgt   1080 

gaaggacacc aaggaggccc tggagaagat tgaggaggag cagaacaagt ccaagaagaa   1140 

ggcccagcag gctgctgctg gcacaggcaa ctccagccag gtgtcccaga actaccccat   1200 

tgtgcagaac ctccagggcc agatggtgca ccaggccatc tccccccgga ccctgaatgc   1260 

ctgggtgaag gtggtggagg agaaggcctt ctcccctgag gtgatcccca tgttctctgc   1320 

cctgtctgag ggtgccaccc cccaggacct gaacaccatg ctgaacacag tggggggcca   1380 

tcaggctgcc atgcagatgc tgaaggagac catcaatgag gaggctgctg agtgggacag   1440 

gctgcatcct gtgcacgctg gccccattgc ccccggccag atgagggagc ccaggggctc   1500 

tgacattgct ggcaccacct ccaccctcca ggagcagatt ggctggatga ccaacaaccc   1560 

ccccatccct gtgggggaaa tctacaagag gtggatcatc ctgggcctga acaagattgt   1620 

gaggatgtac tcccccacct ccatcctgga catcaggcag ggccccaagg agcccttcag   1680 

ggactatgtg gacaggttct acaagaccct gagggctgag caggcctccc aggaggtgaa   1740 

gaactggatg acagagaccc tgctggtgca gaatgccaac cctgactgca agaccatcct   1800 

gaaggccctg ggccctgctg ccaccctgga ggagatgatg acagcctgcc agggggtggg   1860 

gggccctggt cacaaggcca gggtgctggc tgaggccatg tcccaggtga ccaactccgc   1920 

caccatcatg atgcagaggg gcaacttcag gaaccagagg aagacagtga agtgcttcaa   1980 

ctgtggcaag gtgggccaca ttgccaagaa ctgtagggcc cccaggaaga agggctgctg   2040 

gaagtgtggc aaggagggcc accagatgaa ggactgcaat gagaggcagg ccaacttcct   2100 

gggcaaaatc tggccctccc acaagggcag gcctggcaac ttcctccagt ccaggcctga   2160 

gcccacagcc cctcccgagg agtccttcag gtttggggag gagaagacca cccccagcca   2220 

gaagcaggag cccattgaca aggagctgta ccccctggcc tccctgaggt ccctgtttgg   2280 

caacgacccc tcctcccagt aaaataaagc ccgggcagat ctgatctgct gtgccttcta   2340 

gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca   2400 

ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc   2460 

attctattct ggggggtggg gtggggcagc acagcaaggg ggaggattgg gaagacaata   2520 

gcaggcatgc tggggatgcg gtgggctcta                                    2550 

 
           
             5  
             2662  
             DNA  
             Artificial Sequence  
             
               SEAP expression cassette  
             
           
            5 

ccattgcata cgttgtatcc atatcataat atgtacattt atattggctc atgtccaaca     60 

ttaccgccat gttgacattg attattgact agttattaat agtaatcaat tacggggtca    120 

ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct    180 

ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta    240 

acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac    300 

ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt    360 

aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag    420 

tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat    480 

gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat    540 

gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc    600 

ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt    660 

ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga    720 

caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt    780 

gccaagagtg agatctaagt aagcttcctg catgctgctg ctgctgctgc tgctgggcct    840 

gaggctacag ctctccctgg gcatcatccc agttgaggag gagaacccgg acttctggaa    900 

ccgcgaggca gccgaggccc tgggtgccgc caagaagctg cagcctgcac agacagccgc    960 

caagaacctc atcatcttcc tgggcgatgg gatgggggtg tctacggtga cagctgccag   1020 

gatcctaaaa gggcagaaga aggacaaact ggggcctgag atacccctgg ccatggaccg   1080 

cttcccatat gtggctctgt ccaagacata caatgtagac aaacatgtgc cagacagtgg   1140 

agccacagcc acggcctacc tgtgcggggt caagggcaac ttccagacca ttggcttgag   1200 

tgcagccgcc cgctttaacc agtgcaacac gacacgcggc aacgaggtca tctccgtgat   1260 

gaatcgggcc aagaaagcag ggaagtcagt gggagtggta accaccacac gagtgcagca   1320 

cgcctcgcca gccggcacct acgcccacac ggtgaaccgc aactggtact cggacgccga   1380 

cgtgcctgcc tccgcccgcc aggaggggtg ccaggacatc gctacgcagc tcatctccaa   1440 

catggacatt gacgtgatcc taggtggagg ccgaaagtac atgtttcgca tgggaacccc   1500 

agaccctgag tacccagatg actacagcca aggtgggacc aggctggacg ggaagaatct   1560 

ggtgcaggaa tggctggcga agcgccaggg tgcccggtat gtgtggaacc gcactgagct   1620 

catgcaggct tccctggacc cgtctgtgac ccatctcatg ggtctctttg agcctggaga   1680 

catgaaatac gagatccacc gagactccac actggacccc tccctgatgg agatgacaga   1740 

ggctgccctg cgcctgctga gcaggaaccc ccgcggcttc ttcctcttcg tggagggtgg   1800 

tcgcatcgac catggtcatc atgaaagcag ggcttaccgg gcactgactg agacgatcat   1860 

gttcgacgac gccattgaga gggcgggcca gctcaccagc gaggaggaca cgctgagcct   1920 

cgtcactgcc gaccactccc acgtcttctc cttcggaggc taccccctgc gagggagctc   1980 

catcttcggg ctggcccctg gcaaggcccg ggacaggaag gcctacacgg tcctcctata   2040 

cggaaacggt ccaggctatg tgctcaagga cggcgcccgg ccggatgtta ccgagagcga   2100 

gagcgggagc cccgagtatc ggcagcagtc agcagtgccc ctggacgaag agacccacgc   2160 

aggcgaggac gtggcggtgt tcgcgcgcgg cccgcaggcg cacctggttc acggcgtgca   2220 

ggagcagacc ttcatagcgc acgtcatggc cttcgccgcc tgcctggagc cctacaccgc   2280 

ctgcgacctg gcgccccccg ccggcaccac cgacgccgcg cacccgggtt aacccgtggt   2340 

ccccgcgttg cttcctctgc tggccgggac atcaggtggc ccccgctgaa ttggaatcga   2400 

tcagaattca gtcgacgata tctgatcacg atctgatctg ctgtgccttc tagttgccag   2460 

ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact   2520 

gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt   2580 

ctggggggtg gggtggggca gcacagcaag ggggaggatt gggaagacaa tagcaggcat   2640 

gctggggatg cggtgggctc ta                                            2662 

 
           
             6  
             35101  
             DNA  
             adenovirus serotype 17  
             
               n = a, t, c or g  
             
           
            6 

catcatcaat aatatacccc acaaagtaaa caaaagttaa tatgcaaatg aggttttaaa     60 

tttagggcgg ggctactgct gattggccga gaaacgttga tgcaaatgac gtcacgacgc    120 

acggctaacg gtcgccgcgg aggcgtggcc tagcccggaa gcaagtcgcg gggctgatga    180 

cgtataaaaa agcggacttt aaacccggaa acggccgatt ttcccgcggc cacgcccgga    240 

tatgaggtaa ttctgggcgg atgcaagtga aattaggtca ttttggcgcg aaaactgaat    300 

gaggaagtga aaagtgaaaa ataccggtcc cgcccagggc ggaatattta ccgagggccg    360 

agagactttg accgattacg tgtgggtttc gattgcggtg ttttttcgcg aatttccgcg    420 

tccgtgtcaa agtccggtgt ttatgtcaca gatcagctga tccacagggt atttaaacca    480 

gtcgagcccg tcaagaggcc actcttgagt gccagcgagt agagatttct ctgagctccg    540 

ctcccagagt gtgagaaaaa tgagacacct gcgcctcctg cctggaactg tgcccttgga    600 

catggccgca ttattgctgg atgactttgt gagtacagta ttggaggatg aactgcaacc    660 

aactccgttc gagctgggac ccacacttca ggacctctat gatttggagg tagatgccca    720 

ggaggacgac ccgaacgaag atgctgtgaa tttaatattt ccagaatctc tgattcttca    780 

ggctgacata gccagcgaag ctctacctac tccacttcat actccaactc tgtcacccat    840 

acctgaattg gaagaggagg acgagttaga cctccggtgt tatgaggaag gttttcctcc    900 

cagcgattca gaggacgaac agggtgagca gagcatggct ctaatctcag actatgcttg    960 

tgtggttgtg gaagagcatt ttgtgttgga caatcctgag gtgcccgggc aaggctgtaa   1020 

atcctgccag taccaccggg ataagaccgg agacacgaac gcctcctgtg ctctgtgtta   1080 

catgaaaaag aacttcagct ttatttacag taagtggagt gaatgtgaga gaggctgagt   1140 

gcttaagaca taactgggtg atgcttcaac agctgtgcta agtgtggttt attttgtttc   1200 

taggtccggt gtcagaggat ggtcatcacc ctcagaagaa gaccacccgt gtccccctga   1260 

tctgtcaggc gaaacgcccc tgcaagtgca cagacccacc ccagtcagac ccagtggcga   1320 

gaggcgagca gctgttgaaa aaattgagga cttgttacat gacatgggtg gggatgaacc   1380 

tttggacctg agcttgaaac gtcccaggaa actaggcgca gctgcgctta gtcatgtgta   1440 

aataaagttg tacaataaaa attatatgtg acgcatgcaa ggtgtggttt atgactcatg   1500 

ggcggggctt agttctatat aagtggcaac acctgggcac tggagcacag accttcaggg   1560 

agttcctgat ggatgtgtgg actatccttg cagactttag caagacacgc cggcttgtag   1620 

aggatagttc agacgggtgc tccgggttct ggagacactg gtttggaact cctctatctc   1680 

gcctggtgta cacagttaaa aaggattata acgaggaatt tgaaaatctt tttgctgatt   1740 

gctctggcct gctagattct ctgaatctcg gccaccagtc ccttttccag gaaagggtac   1800 

tccacagcct tgatttttcc agcccagggc gcactacagc cggggttgct tttgtggttt   1860 

ttctggttga caaatggagc cagaacaccc aactgagcag gggctacatt ctggacttcg   1920 

cagccatgca cctgtggagg gcatgggtca ggcagcgggg acagagaatc ttgaactact   1980 

ggcttctaca gccagcagct ccgggtcttc ttcgtctaca cagacaaaca tccatgttgg   2040 

aggaagaaat gaggcaggcc atggacgaga acccgaggag cggtctggac cctccgtcgg   2100 

aagaggagtt ggattgaatc aggtatccag cctgtaccca gagcttagca aggtgctgac   2160 

atccatggcc aggggagtga agagggagag gagcgatggg ggcaataccg ggatgatgac   2220 

cgagctgacg gccagtctga tgaatcgcaa gcgcccagag cgccttacct ggtacgagct   2280 

acagcaggag tgcagggatg agttgggcct gatgcaggat aaatatggcc tggagcagat   2340 

aaaaacccat tggttgaacc cagatgagga ttgggaggag gctattaaga agtatgccaa   2400 

gatagccctg cgcccagatt gcaagtacat agtgaccaag accgtgaata tcagacatgc   2460 

tgctacatct cggggaacgg ggcagaggtg gtcattgata ccctggacaa ggccgccttt   2520 

aggtgttgca tgatgggaat gagagccgga gtgatgaata tgaattccat gatctttatg   2580 

aacatgaagt tcaatggaga gaagtttaat ggggtgctgt tcatggccaa cagccacatg   2640 

accctgcatg gctgcgactt tttcggcttt aacaatatgt gcgcagaggt ctggggcgct   2700 

tccaagatca ggggatgtaa gttttatggc tgctggatgg gcgtggtcgg aagacccaag   2760 

agcgagatgt ctgtgaagca gtgtgtgttt gagaaatgct acctgggagt ctctaccgag   2820 

ggcaatgcta gagtgaggca ctgctcttcc ctggagacgg gctgcttctg cctggtgaag   2880 

ggcacagcct ctctgaagca taatatggtg aagggctgca cggatgagcg catgtacaac   2940 

atgctgactg cgactcgggg gtctgtcata tcctgaagaa catccatgtg acctcccacc   3000 

ccagaaagaa gtggccagtg tttgagaata acatgctgat caagtgccac atgcacctgg   3060 

gcgccagaag gggcaccttc cagccgtacc agtgcaactt tagccagacc aagctgctgt   3120 

tggagaacga tgccttctcc agggtgaacc tgaacggcat ctttgacatg gatgtctcgg   3180 

tgtacaagat cctgagatac gatgagacca agtccagggt gcgcgcttgc gagtgcgggg   3240 

gcagacacac caggatgcag ccagtggccc tggatgtgac cgaggagctg agaccagacc   3300 

acctggtgat ggcctgtacc gggaccgagt tcagctccag tggggaggac acagattaga   3360 

ggtaggtttg agtagtgggc gtggctaagg tgactataaa ggcgggtgtc ttacgagggt   3420 

ctttttgctt ttctgcagac atcatgaacg ggaccggcgg ggccttcgaa ggggggcttt   3480 

ttagccctta tttgacaacc cgcctgccag gatgggccgg agttcgtcag aatgtgatgg   3540 

gatcgacggt ggacgggcgc ccagtgcttc cagcaaattc ctcgaccatg acctacgcga   3600 

ccgtggggaa ctcgtcgctt gacagcaccg ccgcagccgc ggcagccgca gccgccatga   3660 

cagcgacgag actggcctcg agctacatgc ccagcagcag cagtagcccc tctgtgccca   3720 

gttccatcat cgccgaggag aactgctggc cctgctggcc gagctggaag ccctgagccg   3780 

ccagctggcc gccctgaccc agcaggtgtc cgagctccgc gaacagcagc agcaaaataa   3840 

atgattcaat aaacacatat tctgattcaa acagcaaagc atctttatta tttatttttt   3900 

cgcgcgcggt aggccctggt ccacctctcc cgatcattga gagtgcggtg gattttttcc   3960 

aagacccggt agaggtggga ttggatgttg aggtacatgg gcatgagccc gtcccggggg   4020 

tggaggtagc accactgcat ggcctcgtgc tctggggtcg tgttgtagat gatccagtca   4080 

tagcaggggc gctgggcgtg gtgctggatg atgtccttga ggaggagact gatggccacg   4140 

gggagcccct tggtgtaggt gttggcaaag cggttgagct gggagggatg catgcggggg   4200 

gagatgatgt gcagtttggc ctggatcttg aggttggcga tgttgccacc cagatcccgc   4260 

cgggggttca tgttgtgcag gaccaccagg acggtgtagc ccgtgcactt ggggaactta   4320 

tcatgcaact tggaagggaa tgcgtggaag aatttggaga cgcccttgtg cccgcccagg   4380 

ttttccatgc actcatccat gatgatggcg atgggcccgt gggctgcggc tttggcaaag   4440 

acgtttctgg ggtcagagac atcataatta tgctcctggg tgagatcatc ataagacatt   4500 

ttaatgaatt ttgggcggag ggtgccagat tgggggacga tggtttccct cgggccccgg   4560 

ggcgaagttc ccctcgcaga tctgcatctc ccaggctttc atctcggagg gggggatcat   4620 

gtccacctgc ggggcgatga aaaaaacggt ttccggggcg ggggtgatga gctgcgagga   4680 

gagcaggttt ctcaacagct gggacttgcc gcacccggtc gggccgtaga tgaccccgat   4740 

gacgggttgc aggtggtagt tcaaggacat gcagctgccg tcgtcccgga ggaggggggc   4800 

cacctcgttg agcatgtctc taacttggag gttttcccgg acgagctcgc cgaggaggcg   4860 

gtccccgccc agcgagagga gctcttgcag ggaagcaaag tttttcaggg gcttgagtcc   4920 

gtcggccatg ggcatcttgg cgagggtctg cgagaggagt tcgagacgtc ccagagctcg   4980 

gtgacgtgct ctacggcatc tcgatccagc agacttcctc gtttcggggg ttgggacgac   5040 

tgcgactgta gggcacgaga cgatgggcgt ccagcgcggc cagcgtcatg tccttccagg   5100 

gtctcagggt ccgcgtgagg gtggtctccg tcacggtgaa ggggtgggcc cctggctggg   5160 

cgcttgcaag ggtgcgcttg agactcatcc tgctggtgct gaaacgggca cggtcttcgc   5220 

cctgcgcgtc ggcgagatag cagttgacca tgagctcgta gttgagggcc tcggcggcgt   5280 

ggcccttggc gcggagcttg cccttggaag agcgtccgca ggcgggacag aggagggatt   5340 

gcagggcgta gagcttgggc gcaagaaaga ccgactcggg agcaaaagcg tccgctccgc   5400 

agtgggcgca gacggtctcg cactcgacga gccaggtgag ctcgggctgc tcggggtcaa   5460 

aaaccagttt tcccccgttc tttttgatgc gcttcttacc tcgcgtctcc atgagtctgt   5520 

gtccgcgctc ggtgacaaac aggctgtcgg tgtccccgta gacggacttg attggcctgt   5580 

cctgcagggg cgtcccgcgg tcctcctcgt agagaaactc ggaccactct gagacaaagg   5640 

cgcgcgtcca cgccaagaca aaggaggcca cgtgcgaggg gtagcggtcg ttgtccacca   5700 

gggggtccac cttttccacc gtgtgcagac acatgtcccc ttcctccgca tccaagaagg   5760 

tgattggctt gtaggtgtag gccacgtgac caggggtccc cgacgggggg gtataaaagg   5820 

gggcgggtct gtgctcgtcc tcactctctt ccgcgtcgct gtccacgagc gccagctgtt   5880 

ggggtaggta ttccctctcg agagcgggca tgacctcggc actcaggttg tcagtttcta   5940 

gaaacgagga ggatttgatg ttggcttgcc ctgccgcaat gctttttagg agactttcat   6000 

ccatctggtc agaaaagact atttttttat tgtcaagctt ggtggcaaag gagccataga   6060 

gggcgttgga gagaagcttg gcgatggatc tcatggtctg atttttgtca cggtcggcgc   6120 

gctccttggc cgcgatgttg agctggacat attcgcgcgc gacacacttc cattcgggaa   6180 

agacggtggt gcgctcgtcg ggcacgatcc tgacgcgcca gccgcggtta tgcagggtga   6240 

ccaggtccac gctggtggcc acctcgccgc gcaggggctc gttagtccag cagagtctgc   6300 

cgcccttgcg cgagcagaac gggggcagca catcaagcag atgctcgtca ggggggtccg   6360 

catcgatggt gaagatgccg ggacagagtt tcttgtcaaa atagtctatt tttgaggatg   6420 

catcatccaa ggccatctgc cactcgcggg cggccattgc tcgctcgtag gggttgaggg   6480 

gcggacccca cggcatggga tgcgtgaggg cggaggcgta catgccgcaa atgtcgtaaa   6540 

catagatggg ctccgagaag atgccgatgt tggtgggata acagcgcccc ccgcggatgc   6600 

tggcgcgcac gtattcatac aactcgtgcg aggggccaag aaggccgggg ccgaaattgg   6660 

tgcgctgggg ctgctcggcg cggaaaacaa tctggcgaaa gatggcgtgc gagttggagg   6720 

agatggtggg ccgttggaag atgttaaagt gggcgtgggg caagcggacc gagtcgcgga   6780 

tgaagtgcgc gtaggagtct tgcagcttgg cgacgaactc ggcggtgacg agaacgtcca   6840 

tggcgcagta gtccagcgtt tcgcggatga tgtcataacc cgcctctcct ttcttctccc   6900 

acagctcgcg gttgagggcg tattcctcgt catccttcca gtactcccgg agcgggaatc   6960 

ctcgatcgtc cgcacggtaa gagcccagca tgtagaaatg gttcacggcc ttgtagggac   7020 

agcagccctt ctccacgggg agggcgtaag cttgtgcggc cttgcggagc gaggtgtgcg   7080 

tcagggcgaa ggtgtccctg accatgactt tcaagaactg gtacttgaaa tccgagtcgt   7140 

cgcagccgcc gtgctcccat agctcgaaat cggtgcgctt cttcgagagg gggttaggca   7200 

gagcgaaagt gacgtcattg aagagaatct tgcctgctcg cggcatgaaa ttgcgggtga   7260 

tgcggaaagg gcccgggacg gaggctcggt tgttgatgac ctgggcggcg aggacgatct   7320 

cgtcgaagcc gttgatgttg tgcccgacga tgtagagttc catgaatcgc gggcggcctt   7380 

tgatgtgcgg cagctttttg agctcctcgt aggtgaggtc ctcggggcat tgcaggccgt   7440 

gctgctcgag cgcccattcc tggagatgtg ggttggcttg catgaaggaa gcccagagct   7500 

cgcgggccat gagggtctgg agctcgtcgc gaaagaggcg gaactgctgg cccacggcca   7560 

tcttttcggg tgtgacgcag tagaaggtga gggggtcccg ctcccagcga tcccagcgta   7620 

agcgcgcggc tagatcgcga gcaagggcga ccagctctgg gtcccccgag aatttcatga   7680 

ccagcatgaa ggggacgagc tgcttgccga aggaccccat ccaggtgtag gtttctacat   7740 

cgtaggtgac aaagagccgc tccgtgcgag gatgagagcc gattgggaag aactggattt   7800 

cctgccacca gttggacgag tggctgttga tgtgatgaaa gtagaaatcc cgccggcgaa   7860 

ccgagcactc gtgctgatgc ttgtaaaagc gtccgcagta ctcgcagcgc tgcacgggct   7920 

gtacctcatc cacgagatac acagcgcgtc ccttgaggag gaacttcagg agtggcggcc   7980 

ctggctggtg gttttcatgt tcgcctgcgt gggactcacc ctggggctcc tcgaggacgg   8040 

agaggctgac gagcccgcgc gggagccagg tccagatctc ggcgcggcgg gggcggagag   8100 

cgaagacgag ggcgcgcagt tgggagctgt ccatggtgtc gcggagatcc aggtccgggg   8160 

gcagggttct gaggttgacc tcgtagaggc gggtgagggc gtgcttgaga tgcagatggt   8220 

acttgatttc tacgggtgag ttggtggccg tgtccacgca ttgcatgagc ccgtagctgc   8280 

gcggggccac gaccgtgccg cggtgcgctt ttagaagcgg tgtcgcggac gcgctcccgg   8340 

cggcagcggc ggttccggcc ccgcgggcag gggcggcaga ggcacgtcgg cgtggcgctc   8400 

gggcaggtcc cggtgttgcg ccctgagagc gctggcgtgc gcgacgacgc ggcggttgac   8460 

atcctggatc tgccgcctct gcgtgaagac cactggcccc gtgactttga acctgaaaga   8520 

cagttcaaca gaatcaatct cggcgtcatt gacggcgggc tgacgcagga tctcttgcac   8580 

gtcgcccgag ttgtcctggt aggcgatctc ggacatgaac tgctcgatct cctcctcctg   8640 

gagatcgccg cgacccgcgc gctccacggt ggcggcgagg tcattcgaga tgcgacccat   8700 

gagctgcgag aaggcgccca ggccgctctc gttccagacg cggctgtaga ccacgtcccc   8760 

gtcggcgtcg cgcgcgcgca tgaccacctg cgcgaggttg agctccacgt gccgcgcgaa   8820 

gacggcgtag ttgcgcaggc gctggaagag gtagttgagg gtggtggcga tgtgctcggt   8880 

gacgaagaag tacatgatcc agcggcgcag gggcatctcg ctgatgtcgc cgatggcctc   8940 

cagcctttcc atggcctcgt agaaatccac ggcgaagttg aaaaactggg cgttgcgggc   9000 

cgagaccgtg agctcgtctt ccaggagcct gatgagctcg gcgatggtgg cgcgcacctc   9060 

gcgctcgaaa tccccggggg cctcgtcctc ttcctcttct tccatgacaa cctcttctat   9120 

ttcttcctct gggggcggtg gtggtggcgg ggcccgacga cgacggcgac gcaccgggag   9180 

acggtcgacg aagcgctcga tcatctcccc gcggcggcga cgcatggttt cggtgacggc   9240 

gcgaccccgt tcgcgaggac gcagcgtgaa gacgccgccg gtcatctccc ggtaatgggg   9300 

cgggtccccg ttgggcagcg agagggcgct gacgatgcat cttatcaatt gcggtgtagg   9360 

ggacgtgagc gcgtcgagat cgaccggatc ggagaatctt tcgaggaaag cgtctagcca   9420 

atcgcagtcg caaggtaagc tcaaacacgt agcagccctg tggacgctgt tagaattgcg   9480 

gttgctaatg atgtaattga agtaggcgtt tttgaggcgg cggatggtgg cgaggaggac   9540 

caggtccttg ggtcccgctt gctggatgcg gagccgctcg gccatgcccc aggcctggcc   9600 

ctgacaccgg cttaggttct tgtagtagtc atgcatgagc ctctcgatgt catcactggc   9660 

ggaggcggag tcttccatgc gggtgacccc gacgcccctg agcggctgca cgagcgccag   9720 

gtcggcgacg acgcgctcgg cgaggatggc ctgttgcacg cgggtgaggg tgtcctggaa   9780 

gtcgtccatg tcgacgaagc ggtggtaggc ccctgtgttg atggtgtaag tgcagttggc   9840 

catgagcgac cagttgacgg tctgcaggcc gggctgcacg acctcggagt acctgagccg   9900 

cgagaaggcg cgcgagtcga agacgtagtc gttgcaggtg cgcacaaggt actggtatcc   9960 

gactaggaag tgcggcggcg gctggcggta gagcggccag cgctgggtgg ccggcgcgcc  10020 

cggggccagg tcctcgagca tgaggcggtg gtagccgtag aggtagcggg acatccaggt  10080 

gatgccggca gcggtggtgg aggcgcgcgg gaactcgcgg acgcggttcc agatgttgcg  10140 

cagcggcagg aaatagtcca tggtcggcac ggtctggccg gtgagacgcg cgcagtcatt  10200 

gacgctctag aggcaaaaac gaaagcggtt gagcgggctc ttcctccgta gcctggcgga  10260 

acgcaaacgg gttaggccgc gcgtgtaccc cggttcgagt cccctcgaat caggctggag  10320 

ccgcgactaa cgtggtattg gcactcccgt ctcgacccga gcccgatagc cgccaggata  10380 

cgcgggaaga gccctttttg ccggccgarg ggagtcgcta gacttgaaag cggccgaaaa  10440 

ccccgccggg tagtggctcg cgcccgtagt ctggagaagc atcgccaggg ttgagtcgcg  10500 

gcagaacccg gttcgcggac ggccgcggcg agcgggactt ggtcaccccg ccgatttaaa  10560 

gacccacagc cagccgactt ctccagttac gggagcgagc cccctttttt ctttttgcca  10620 

gatgcatccc gtcctgcgcc aaatgcgtcc cacccccccg gcgaccaccg cgaccgcggc  10680 

cgtagcaggc gccggcgcta gccagccaca gccacagaca gagatggact tggaagaggg  10740 

cgaagggctg gcgagactgg gggcgccttc cccggagcga cacccccgcg tgcagctgca  10800 

gaaggacgtg cgcccggcgt acgtgcctgc gcaaaacctg ttcagggacc gcagcgggga  10860 

ggagcccgag gagatgcgcg actgccggtt tcgggcgggc agggagctgc gcgagggcct  10920 

ggaccgccag cgcgtgctgc gcgacgagga tttcgagccg aacgagcaga cggggatcag  10980 

ccccgcgcgc gcgcacgtgg cggcggccaa cctggtgacg gcctacgagc agacggtgaa  11040 

gcaggagcgc aacttccaaa agagtttcaa caaccatgtg cgcaccctga tcgcgcgcga  11100 

ggaggtggcc ctgggcctga tgcacctgtg ggacctggcg gaggccatcg tgcagaaccc  11160 

ggacagcaag cctctgacgg cgcagctgtt cctggtggta cagcacagca gggacaacga  11220 

ggcgttcagg gaggcgctgc taaacatcgc cgagcccgag ggtcgctggc tgctggagct  11280 

gatcaacatc ttgcagagca tcgtagttca ggagcgcagc ctgagcttgg ccgagaaggt  11340 

ggcggcaatc aactactcgg tgcttagcct gggcaagttt tacgcgcgca agatttacaa  11400 

gacgccgtac gtgcccatag acaaggaggt gaagatagac agcttttaca tgcgcatggc  11460 

gctcaaggtg ctgacgctga gcgacgacct gggcgtgtac cgcaacgacc gcatccacaa  11520 

ggccgtgagc gcgagccggc ggcgcgagct gagcgaccgc gagctgatgc tgagcctgcg  11580 

ccgggcgctg gtagggggcg ccgccggcgg cgaggagtcy tacttcgaca tgggggcgga  11640 

cctgcattgg cagccgagcc ggcgcgcctt ggaggccgcc tacggtccag aggacttgga  11700 

tgaggaagag gaagaggagg aggatgcacc cgctgcgggg tactgacgcc tccgtgatgt  11760 

gtttttagat gcagcaagcc ccggaccccg ccataagggc ggcgctgcaa agccagccgt  11820 

ccggtctagc atcggacgac tgggaggctg cgatgcaacg catcatggcc ctgacgaccc  11880 

gcaaccccga gtcctttaga caacagccgc aggccaacag actctcggcc attctggagg  11940 

cggtggtccc ttctcggacc aaccccacgc acgagaaggt gctggcgatc gtgaacgcgc  12000 

tggcggagaa caaggccatc cgtcccgacg aggccgggct agtgtacaac gccctgctgg  12060 

agcgcgtagg ccgctacaac agcacaaacg tgcagtccaa cctggaccgg ctggtgacgg  12120 

acgtgcgcga agccgtggcg cagcgcgagc ggttcaagaa cgagggcctg ggctcgctgg  12180 

tggcgctgaa cgccttcctg gcgacgcagc cggcgaacgt gccgcgcggg caggatgatt  12240 

acaccaactt tatcagcgcg ctgcggctga tggtgaccga ggtgccccag agcgaggtgt  12300 

accagtcggg cccggactac tttttccaaa ctagcagaca gggcctgcaa acggtgaacc  12360 

tgagccaggc tttcaagaac ctgcgcgggc tgtggggcgt gcaggcgccc gtgggcgacc  12420 

ggtcgacggt gagcagcttg ctgacgccca actcgcggct gctgctgctg ctgatcgcgc  12480 

ccttcaccga cagtggcagc gtaaaccgca actcgtacct gggtcacctg ctaacgctgt  12540 

accgcgaggc cataggccag gcgcaggtgg acgagcagac cttccaggag atcactagcg  12600 

tgagccgcgc gctggggcag aacgacaccg acagtctgag ggccaccctg aacttcttgc  12660 

tgaccaatag acagcagaag atcccggcgc agtacgcgct gtcggccgag gaggagcgca  12720 

tcctgagata tgtgcagcag agcgtagggc ttttcctgat gcaggagggg gccactccca  12780 

gcgccgcgct ggacatgacc gcgcgcaaca tggaacctag catgtacgcc gccaaccggc  12840 

cgtttatcaa taagctaatg gactacctgc atcgcgcggc gtccatgaac tcggactact  12900 

ttaccaatgc cattttgaac ccgcactggc ttccgccgcc ggggttctat acgggcgagt  12960 

acgacatgcc cgaccccaac gacgggtttt tgtgggacga cgtggacagc gcggtgtttt  13020 

caccgacctt gcaaaagcgc caggaggcgg tgcgcacgcc cgcgagcgag ggcgcggtgg  13080 

gtcggagccc ctttcctagc ttagggagtt tgcatagctt gccgggctct gtgaacagcg  13140 

gcagggtgag ccggccgcgc ttgctgggcg aggacgagta cctgaacgac tcgctgctgc  13200 

agccgccgcg ggtcaagaac gccatggcca ataacgggat agagagtctg gtggacaaac  13260 

tgaaccgctg gaagacctac gctcaggacc atagggagcc tgcgcccgcg ccgcggcgac  13320 

agcgccacga ccggcagcgg ggcctggtgt gggacgacga ggactcggcc gacgatagca  13380 

gcgtgttgga cttgggcggg agcggtgggg tcaacccgat atcgcgcatc ctgcagccca  13440 

aactggggcg acggatgttt tgaatgcaaa ataaaactca ccaaggccat agcgtgcgtt  13500 

ctcttccttg ttagagatga ggcgtgcggt ggtgtcttcc tctcctcctc cctcgtacga  13560 

gagcgtgatg gcgcaggcga ccctggaggt tccgtttgtg cctccgcggt atatggctcc  13620 

tacggagggc agaaacagca ttcgttactc ggagctggct ccgttgtacg acaccactcg  13680 

cgtgtacttg gtggacaaca agtcggcgga catcgcttcc ctgaactatc aaaacgacca  13740 

cagcaacttc ctgaccacgg tggtgcagaa caacgatttc acccccgccg aggctagcac  13800 

gcagacgata aattttgacg agcggtcgcg gtggggcggt gatctgaaga ccattctgca  13860 

caccaacatg cccaatgtga acgagtacat gttcaccagc aagtttaagg cgcgggtgat  13920 

ggtggctaga aaacacccac agggggtaga agcaacagat ttaagcaagg atatcttaga  13980 

gtatgagtgg tttgagttta ccctgcccga gggcaacttt tccgagacca tgaccataga  14040 

cctgatgaac aacgccatct tggaaaacta cttgcaagtg gggcggcaaa atggcgtgct  14100 

ggagagcgat attggagtca agtttgacag cagaaatttc aagctgggct gggaccctgt  14160 

gaccaagctg gtgatgccag gggtctacac ctacgaggcc tttcacccgg acgtggtgct  14220 

gctgccgggc tgcggggtgg acttcacaga gagccgcctg agcaacctcc tgggcattcg  14280 

caagaagcaa cctttccaag agggcttcag aatcatgtat gaggatctag aagggggcaa  14340 

catccccgcc ctgctggatg tgcccaagta cttggaaagc aagaagaagt tagaggaggc  14400 

attggagaat gctgctaaag ctaatggtcc tgcaagagga gacagtagcg tctcaagaga  14460 

ggttgaaaag gcagctgaaa aagaacttgt tattgagccc atcaagcaag atgataccaa  14520 

gagaagttac aacctcatcg agggaaccat ggacacgctg taccgcagct ggtacctgtc  14580 

ctatacctac cgggaccctg agaacggggt gcagtcgtgg acgctgctca ccaccccgga  14640 

cgtcacctgc ggcgcggagc aagtctactg gtcgctgccg gacctcatgc aagaccccgt  14700 

caccttccgt tctacccagc aagtcagcaa ctaccccgtg gtcggcgccg agctcatgcc  14760 

cttccgcgcc aagagctttt acaacgacct cgccgtctac tcccagctca tccgcagcta  14820 

cacctccctc acccacgtct tcaaccgctt ccccgacaac cagatcctct gccgtccgcc  14880 

cgcgcccacc atcaccaccg tcagtgaaaa cgtgcctgct ctcacagatc acgggacgct  14940 

accgctgcgc agcagtatcc gcggagtcca gcgagtgacc gtcactgacg cccgtcgccg  15000 

cacctgtccc tacgtctaca aggccctggg catagtcgcg ccgcgtgtgc tttccagtcg  15060 

caccttctaa aaaatgtcta ttctcatctc gcccagcaat aacaccggct ggggtattac  15120 

taggcccagc agcatgtacg gaggagccaa gaaacgtccc agcagcaccc cgtccgcgtc  15180 

cgcggccact tccgcgctcc gtggggcgct tacaagcgcg ggcggactgc caccgccgcc  15240 

gccgtgcgca ccaccgtcga cgacgtcatc gactcggtgg tcgccgacgc gcgcaactat  15300 

actcccgccc cttcgaccgt ggacgcggtt cattgacagc gtggtggcga cgcggcggcg  15360 

atatgccaga cgcaagagcc ggcgggcgga cggatcgccc aggcgccatt cggagcacgc  15420 

ccgccatggg gcgccgcccg agctctgctg cgccgcgcca gacgcacggg ccgccgggcc  15480 

atgatgcgag ccgcgcgccg cgccgccact gcaccccccg caggcaggac tcgcagacga  15540 

gcggccgccg ccgccgccgc ggccatctct agcatgacca gacccaggcg cggaaacgtg  15600 

tactgggtgc gcgactccgt cacgggcgtg cgcgtgcccg tgcgcacccg tcctcctcgt  15660 

ccctgatcta atgcttgtgt cctcccccgc aagcgacgat gtcaaagcgc atctacaaga  15720 

gagatgctcc aggtcgtcgc cccggagatt tacggaccac cccaggcgga ccagaaaccc  15780 

cgcaaaatca agcgggttaa aaaaaaggat gaggtggacg agggggcagt agagtttgtg  15840 

cgcgagttcg ctccgcggcg gcgcgtaaat tggaaggggc gcaggtgcac gcgtgttgcg  15900 

gcccggcacg gcggtggtgt tcacgcccgg cgagcggtcc tcggtcagga gcaagcgtag  15960 

ctatgacgag gtgtacggcg acgacgacat cctggaccag gcggcagagc gggcgggcga  16020 

gtttgcctac gggaagcggt cgcgcgaaga ggagctgatc tcgctgccgc tggacgagag  16080 

caatcccacg ccgagcctga agcccgtgac ctgcagcagg tgctgcccca ggcggtgctg  16140 

ctgccgagcc gcgggatcaa gcgcgagggc gagaacatgt acccgaccat gcagatcatg  16200 

gtgcccaagc gccggcgcgt ggaggaagtg ctggacaccg tgaaaatgga tgtggagccc  16260 

gaggtcaagg tgcgccccat caagcaggtg gcgccgggcc tgggcgtgca gaccgtggac  16320 

attcagatcc ccaccgacat ggatgtcgac aaaaaaccct cgaccagcat cgaggtgcag  16380 

accgacccct ggctcccagc ctccaccgct accgcttcca cttctaccgt cgccacggtc  16440 

accgagcctc ccaggaggcg aagatggggc cccgccaacc ggctgatgcc caactacgtg  16500 

ttgcatcctt ccattatccc gacgccgggc taccgcggca cccggtacta cgccagccgc  16560 

aggcgcccag ccagcaaacg ccgccgccgc accgccaccc gccgccgtct gccccccgcc  16620 

cgcgtgcgcc gcgtaaccaa cgcgccgggg ccgctcgctc gttctgccca ccgtgcgcta  16680 

ccaccccagc atcctttaat ccgtgtgctg tgatactgtt gcagagagat ggctctcact  16740 

tgccgcctgc gcatccccgt tccgaattac cgaggaagat cccgccgcag gagaggcatg  16800 

gcaggcagcg gcctgaaccg ccgccggcgg cgggccatgc gcaggcgcct gagtggcggc  16860 

tttctgcccg cgctcatccc cataatcgcg gcggccatcg gcacgatccc gggcatagct  16920 

tccgttgcgc tgcaggcgtc gcagcgccgt tgatgtgcga ataaagcctc tttagactct  16980 

gacacacctg gtcctgtata tttttagaat ggaagacatc aattttgcgt ccctggctcc  17040 

gcggcacggc acgcggccgt tcatgggcac ctggaacgag atcggcacca gccagctgaa  17100 

cgggggcgcc ttcaattgga gcagtgtctg gagcgggctt aaaaatttcg gctcgacgct  17160 

ccggacctat gggaacaagg cctggaatag tagcacgggg cagttgttga gggaaaagct  17220 

caaagaccag aacttccagc agaaggtggt ggacggcctg gcctcgggca ttaacggggt  17280 

ggtggacatc gcgaaccagg cagtgcagcg cgagataaac agccgtctgg acccgcggcc  17340 

gcccacggtg gtggagatgg aagatgcaac tcttccgccg ccgaagggcg agaagcggcc  17400 

gcggccagat gcggaggaga cgatcctgca ggtggacgag ccgccttcgt acgaggaggc  17460 

cgtgaaggcc ggcatgccca ccacgcgcat catcgcgcca ctggccacgg gtgtaatgaa  17520 

acccgccacc cttgacctgc ctccaccacc cacgcccgct ccaccgaagg cagctccggt  17580 

tgtgcagccc cctccggtgg cgaccgccgt gcgccgcgtc cccgcccgcc gccaggccca  17640 

gaactggcag agcacgctgc acagtattgt gggcctggga gtgaaaagtc tgaagcgccg  17700 

ccgatgctat tgagagagag gaaggaggac actaaaggga gagcttaact tgtatgtgcc  17760 

ttaccgccag agaacgcgcg aagatggcca ccccctcgat gatgccgcag tgggcgtaca  17820 

tgcacatcgc cgggcaggac gcctcggagt acctgagccc gggtctggtg cagtttgccc  17880 

gcgccaccga cacgtacttc agcctgggca acaagtttag gaaccccacg gtggccccga  17940 

cccacgatgt gaccacggac cggtcccagc gtctgacgct gcgctttgtg cccgtggatc  18000 

gcgaggacac cacgtactcg tacaaggcgc gcttcactct ggccgtgggc gacaaccggg  18060 

tgctagacat ggccagcacg tactttgaca tccgcggcgt cctggaccgc ggtcccagtt  18120 

tcaaacccta ctcgggcacg gcttacaaca gccttgcccc caagggcgct cccaatccca  18180 

gtcagtgggt tgccaaagaa aatggtcagg gaactgataa gacacatact tatggctcag  18240 

ctgccatggg aggaagcaac atcaccattg aaggtttagt aattggaact gatgaaaaag  18300 

ctgaggatgg caaaaaagat atttttgcaa ataaacttta tcagccagaa cctcaagtag  18360 

gtgaagaaaa ctggcaagag tctgaagcct tctatggagg cagagctctt aagaaagaca  18420 

caaaaatgaa gccctgctat ggctcatttg caagacctac caatgaaaaa ggcggacaag  18480 

ctaaatttaa gccagtggaa gaggggcagc aacctaaaga ttatgacata gatttggctt  18540 

tctttgacac acctggaggc accatcacag gaggcacaga cgaagaatat aaagcagaca  18600 

ttgtgttgta cactgaaaat gtcaaccttg aaaccccaga cacccacgtg gtatacaagc  18660 

caggaaaaga ggatgacagt tcagaagtaa atttgacaca gcagtccatg cccaacaggc  18720 

ctaactacat tggcttcaga gacaactttg tgggactcat gtactacaac agtactggca  18780 

acatgggtgt gctggctggt caggcctctc aattgaatgc tgtggtcgac ttgcaagaca  18840 

gaaacaccga gctgtcttac cagctcttgc tagattctct gggtgacaga accagatact  18900 

tcagcatgtg gaactctgcg gtggatagct atgatccaga tgtcaggatc attgaaaatc  18960 

atggtgtgga agatgaactt ccaaactatt gcttcccatt gaatggcact ggcaccaatt  19020 

caacatatct tggcgtaaag gtgaaaccag atcaagatgg tgatgttgaa agcgagtggg  19080 

ataaagatga taccattgca aggcagaatc aaatcgccaa gggcaacgtc tttgccatgg  19140 

agatcaacct ccaggccaac ctgtggaaga gttttctgta ctcgaacgtg gccttgtacc  19200 

tgcccgactc ctacaagtac acgccggcca atgttacgct gcccgccaac accaacacct  19260 

acgagtacat gaacggccgc gtggtagccc cctcgctggt ggacgcctac atcaacatag  19320 

gcgcccgatg gtcgctggac cccatggaca acgtcaaccc cttcaaccac caccgcaatg  19380 

cgggcctgcg ctaccgctcc atgcttctgg gcaacggccg ctacgtgccc ttccacatcc  19440 

aagtgcccca aaagttcttt gccatcaaga acctgctcct gctcccgggc tcctacacct  19500 

acgagtggaa cttccgcaag gatgtcaaca tgatcctgca gagttccctc ggcaacgacc  19560 

tgcgcgtcga cggcgcctcc gtccgcttcg acagcgtcaa cctctacgcc accttcttcc  19620 

ccatggcgca caacaccgcc tccaccctgg aagccatgct gcgcaacgac accaacgacc  19680 

agtccttcaa cgactacctc tcggccgcca acatgctcta ccccatcccg gccaaggcca  19740 

ccaacgtgcc catctccatc ccctcgcgca actgggccgc ttttcgcggc tggagtttca  19800 

cccgtctgaa aaccaaggaa actccctccc tcggctcggg ttttgacccc tactttgtct  19860 

actcgggctc gatcccctac cttgacggac ccttttacct taaccacacc ttcaagaaag  19920 

tctccatcat gttcgactcc tcggtcagct ggcccggcaa cgaccggctg ctcacgccga  19980 

acgagttcga gatcaagcgc agcgtcgacg gggaaggcta caacgtggcc caatgcaaca  20040 

tgaccaagga ctggttcctc gtccagatgc tctcccacta caacatcggc taccagggct  20100 

tccacgtgcc cgagggctac aaggaccgca tgtactcctt cttccgcaac ttccagccca  20160 

tgagcaggca ggtggtcgat gagatcaact acaaggacta caaggccgtc accctgccct  20220 

tccagcacaa caactcgggc ttcaccggct accttgcacc caccatgcgc caagggcagc  20280 

cctaccccgc caacttcccc tacccgctca tcggccagac agccgtgcca tccgtcaccc  20340 

agaaaagtct cctctgcgac agggtcatgt ggcgcatccc cttctccagc aacttcatgt  20400 

ccatgggcgc cttcaccgac ctgggtcaga acatgttcta cgccaactcg gcccacgcgc  20460 

tcgacatgac cttcgaggtg gaccccatgg atgagcccac cgtcctctat cttctcttcg  20520 

aagtgttcga cgtggtcaga gtgcaccagc cgcaccgcgg cgtcatcgag gccgtctacc  20580 

tgcgcacgcc gttctccgcc ggaaacgcca ccacctaagc atgagcggct ccagcgaaag  20640 

agagctcgcg tccatcgtgc gcgacctggg ctgcgggcct actttttggg cacccacgac  20700 

acagcgattc ccgggctttc ttgccggcga caagctggcc tgcgccattg tcaacacggc  20760 

cggccgcgag accggaggcg tgcactggct cgccttcggc tggaacccgc gctcgcgcac  20820 

ctgctacatg ttcgacccct ttgggttctc ggaccgccgg ctcaagcaga tttacagctt  20880 

cgagtacgag gccatgctgc gccgaagcgc cgtggcctct tcgcccgacc gctgtctcag  20940 

cctcgaacag tccacccaga ccgtgcaggg gcccgactcc gccgcctgcg gacttttctg  21000 

ttgcatgttc ttgcatgcct tcgtgcactg gcccgaccga cccatggacg ggaaccccac  21060 

catgaacttg ctgacggggg tgcccaacgg catgctacaa tcgccacagg tgctgcccac  21120 

cctcaggcgc aaccaggagg agctctatcg cttcctcgcg cgccactccc cttactttcg  21180 

ctcccaccgc gccgccatcg aacacgccac cgcttttgac aaaatgaaac aactgcgtgt  21240 

atctcaataa acagcacttt tattttacat gcactggagt atatgcaagt tatttaaaag  21300 

tcgaaggggt tctcgcgctc atcgttgtgc gccgcgctgg ggagggccac gttgcggtac  21360 

tggtacttgg gctgccactt gaactcgggg atcaccagtt tgggcactgg ggtctcgggg  21420 

aaggtctcgc tccacatacg ccggctcatc tgcagggcgc ccagcatgtc cggggcggat  21480 

atcttgaaat cgcagttggg accggtgctc tgcgcgcgcg agttgcggta cacggggttg  21540 

cagcactgga acaccatcag actggggtac tttacgctgg ccagcacgct cttgtcgctg  21600 

atctgatcct tgtccagatc ctcggcgttg ctcacgccga atggggtcat cttgcacagt  21660 

tggcgaccca ggaatggcac gctctgaggc ttgtggttac actcgcagtg cacgggcatc  21720 

agcatcatcc ccgcgccgcg ctgcatattc gggtagaggc cttgacaaag gccgtgatct  21780 

gcttgaaagc ttgttgggcc ttggccccct cgctgaaaaa caggccgcag ctcttcccgc  21840 

tgaactggtt attcccgcac ccggcatcct gcacgcagca gcgcgcgtca tggctggtca  21900 

gttgcaccac gcttcttccc cagcggttct gggtcacctt ggctttgctg ggttgctcct  21960 

tcaacgcgcg ctgcccgttc tcgctggtca catccatctc caccacgtgg tccttgtgga  22020 

tcatcaccgt tccatgcaga cacttgagct ggccttccac ctcggtgcag ccgtgatccc  22080 

acagggcact gccggtgcac tcccagttct tgtgcgcgat cccgctgtgg ctgaagatgt  22140 

aaccttgcaa gaggcgaccc atgatggtgc taaagctctt ctgggtggtg aaggttagtt  22200 

gcagaccgcg ggcctcctcg ttcatccagg tctggcacat cttttggaag atctcggtct  22260 

gctcgggcat gagcttgtaa gcatcgcgca ggccgctgtc gacgcggtaa cgttccatca  22320 

gcacgttcat ggtatccatg cccttttccc aggacgagac cagaggcaga ctcagggggt  22380 

tgcgcacgtt caggacaccg ggggtckcgg gctcgacgat acgttttccg tccttgcctt  22440 

ccttcaacag aaccggaggc tggctgaatc ccactcccac aatcacggca tcttcctggg  22500 

gcatctcttc gtcggggtct accttggtca catgcttggt ctttctggct tgcttctttt  22560 

ttggagggct gtccacgggg accacgtcct ctcggaagac ccggagccca cccgctgata  22620 

ctttcggcgc ttggtgggca gaggaggtgg cggcggcgag gggctcctct cgtgctccgg  22680 

cggatagcgc gccgacccgt ggccccgggg cggagtggcc tctcgctcca tgaaccggcg  22740 

cacgtctgac tgccgccggc cattgtttcc taggggaaga tggaggagca gccgcgtaag  22800 

caggagcagg aggaggactt aaccacccac gagcaaccca aaatcgagca ggacctgggc  22860 

ttcgaagagc cggctcgtct agaaccccac aggatgaaca ggagcacgag caagacgcag  22920 

gccaggagga gaccgacgct gggctcgagc atggctacct gggaggagag gaggatgtgc  22980 

tgctgaaaca cctgcagcgc cagtccctca tcctccggga cgccctggcc gaccggagcg  23040 

aaacccccct cagcgtcgag gagctgtgtc gggcctacga gctcaacctc ttctcgccgc  23100 

gcgtgccccc caaacgccag cccaacggca cctgcgagcc caacccgcgt ctcaacttct  23160 

atcccgtctt tgcggtcccc gaggcccttg ccacctatca catctttttc aagaaccaaa  23220 

agatccccgt ctcctgccgc gccaaccgca cccgcgccga cgcgctcctc gctctggggc  23280 

ccggcgcgcg catacctgat attgcttccc tggaagagtg cccaaaatct tcgaagggct  23340 

cggtcgggac gagacgcgcg cggcgaaacg ctctgaaaga aacagcagag gaagagggtc  23400 

acactagcgc cctggtagag ttggaaggcg acaacgccag gctggccgtg ctcaagcgca  23460 

gcgttgagct cacccacttc gcctaccccg ccgtcaacct cccgcccaag gtcatgcgtc  23520 

gcatcatgga tcagctaatc atgccccaca tcgaggccct cgatgaaagt caggagcagc  23580 

gccccgagga cacccggccc gtggtcagcg atgagcagct tgcgcgctgg cttggtaccc  23640 

gcgaccccca ggccctggag cagcggcgca agctcatgct ggccgtggtc ctggtcaccc  23700 

tcgagctcga atgcatgcga cgctttttca gcgaccccga gacctgcgca aggtcgagga  23760 

gacctgcact acacttttag cacgtttcgt caggcaggca tgcaagatct ccaacgtgga  23820 

gctgaccaac tggtctcctg cctgggaatc ctgcacgaga accgcctggg gcagacagtg  23880 

ctccactcga ccctgaaggg cgaggcgcgg cgggactatg tccgcgactg cgtctttctc  23940 

tttctctgcc acacatggca agctgccatg ggcgtgtggc agcagtgtct cgaggacgag  24000 

aacctgaagg agctggacaa gcttcttgct agaaacctca aaaagctgtg gacgggcttt  24060 

gacgagcgca ccgtcgcctc ggacctggcc gagatcgtcc tcccccgagc gcctgaggca  24120 

gacgctgaaa ggcgggctgc ccgacttcat gagccagagc atgttgcaaa actaccgcac  24180 

tttcattctc gagcgatctg ggatgctgcc cgccacctgc aacgccttcc cctccgactt  24240 

tgtcccgctg agctaccgcg agtgtccccc gccgctgtgg agccactgct acctcttgca  24300 

gctggccaac tacatcgcct accactcgga tgttatcgag gacgtgagcg gcgaggggct  24360 

gctagagtgc cactgccgct gcaacctgtg ctctccgcac cgctcctggt ctgcaacccc  24420 

cagctcctga gcgagaccca ggtcatcggt accttcgagc tgcaaggtcc gcaggagtcc  24480 

accgctccgc tgaaactcac gccggggttg tggacttccg cgtacctgcg caaatttgta  24540 

cccgaggact accacgccca tgagataaag ttcttcgagg accaatcgcg cccgcagcac  24600 

gcggatctca cggcctgcgt catcacccag ggcgcgatcc tcgcccaatt gcacgccatc  24660 

caaaaatccc gccaagagtt tcttttgaaa aagggtagag gggtctatct ggacccccag  24720 

acgggcgaag tgctcaaccc gggtctcccc cagcatgccg aagaagaaca ggagccgcta  24780 

gtggaagaga tggaagaaga atgggacagc cagcagaaga agacgaatgg gaagaagaga  24840 

cagaagaaga agaattggaa aagtggaaga agagcagcac agacaccgtc gccgcaccat  24900 

ccgcgccgca gcccggcggt cacggataca actcgcagtc cgccaagctc ctcgtagatg  24960 

gatcgagtga aggtgacggt aagcacgagc ggcagggcta cgaatcatgg aggcccacaa  25020 

agcgggatca tcgcctgctt gcaagactgc ggggggaaca tcgtttcgcc cgccgctatc  25080 

tgctcttcca tcgcggggtg aacatccccc gcaacgtgtt gcattactac cgtcaccttc  25140 

acagctaaga aaaaatcaga gtaagaggag tcgccggagg aggcntgagg atcgcggcga  25200 

acgagccatt gaccaccagg gagctgagga atcggatctt ccccactctt tatgccattt  25260 

ttcagcagag tcgaggtcag cagcaagagc tcaaagtaaa aaaccggtct ctgcgctcgc  25320 

tcacccgcag ttgcttgtac cacaaaaacg aagatcagct gcagcgcact ctcgaagacg  25380 

ccgaggctct gttccacaag tactgcgcgc tcactcttaa agactaaggc gcgcccaccc  25440 

ggaaaaaagg cgggaattac ctcatcgcca ccatgagcaa ggagattccc accccttaca  25500 

tgtggagcta tcagccccag atgggcctgg ccgcgggcgc ctcccaggac tactccaccc  25560 

gcatgaactg gctcagtgcc ggcccctcga tgatctcacg ggtcaacggg gtccgtaacc  25620 

atcgaaacca gatattgttg gagcaggcgg cggtcacctc aacgcccagg caaagctcaa  25680 

cccgcgtaat tggccctcca ccctggtgta tcaggaaatc cccgggccga ctaccgtact  25740 

acttccgcgt gacgcactgg ccgaagtccg catgactaac tcaggtgtcc agctggccgg  25800 

cggcgcttcc cggtgcccgc tccgcccaca atcgggtata aaaaccctgg tgatacgagg  25860 

cagaggcaca cagctcaacg acgagttggt gagctcttca atcggtctgc gaccggacgg  25920 

agtgttccaa ctagccggag ccgggagatc gtccttcact cccaaccagg ctacctgacc  25980 

ttgcagagca gctcttcgga gcctcgctcc ggaggcatcg gaaccctcca gtttgtggag  26040 

gagtttgtgc cctcggtcta cttcaacccc ttctcgggat cgccaggcct ctacccggac  26100 

gagttcatac cgaacttcga cgcagtgaga gaagcggtgg acggccacga ctgaatgtct  26160 

tatggtgact cggctgagct cgctcggttg aggcacctag accactgccg ccgcctgcgc  26220 

tgcttcgccc gggagagctg cggacttatc tactttgagt ttcccgagga gcaccccaac  26280 

ggccctgcac acggagtgcg gatcaccgta gagggcacca ccgagtctca cctggttagg  26340 

ttcttcaccc agcaaccctt cctggtcgag cgggaccggg gaggcaccac ctacaccgtc  26400 

tactgcatct gtccaacccc gaagttgcat gagaattttt gttgtactct gtgtgctgag  26460 

tttaataaaa gctaaactcc tacaatactc tgggatcccg tgtcgtcgca ctcgcaacaa  26520 

gaccttcaac ctcaccaacc agactgaggt aaaattcaac tgcagaccgg gggacaaata  26580 

catcctctgg ctttttaaaa acacttcctt cgcagtctcc aacgcctgcg ccaacgacgg  26640 

tattgaaata cccaacaacc ttaccagtgg actaacttat actaccagaa agactaagct  26700 

agtactctac aatccttttg tagagggaac ctaccactgc cagagcggac cttgcttcca  26760 

cactttcact ttggtgaacg ttaccgacag cagcacagcc gctacagaaa catctaacct  26820 

tctttttgat actaacactc ctaaaaccgg aggtgagctc tgggttccct ctctaacaga  26880 

ggggggtaaa catattgaag cggttgggta tttgatttta ggggtggtcc tgggtgggtg  26940 

catagcggtg ctgtattacc ttccttgctg gatcgaaatc aaaatcttta tctgctgggt  27000 

cagacattgt tgggaggaac catgaagggg ctcttgctga ttatcctttc cctggtgggg  27060 

ggtgtactgt catgccacga acagccacga tgtaacatca ccacaggcaa tgagaggagt  27120 

gtgatatgca cagtagtcat caaatgcgag catacatgcc ctctcaacat cacattcaaa  27180 

aaccgtacca tgggaaatgc atgggtgggc gactgggaac caggagatga gcagaactac  27240 

acggtcactg tccatggtag caatggaaat cacacttttg gtttcaaatt catttttgaa  27300 

gtcatgtgtg atatcacact gcatgtggct agacttcatg gcttgtggcc ccctaccaag  27360 

gataacatgg ttgggttttc tttggctttt gtgatcatgg cctgtgcaat gtcaggtctg  27420 

ctggtagggg ctttagtgtg gttcctaaag cgcaagccta ggtatggaaa tgaggagaag  27480 

gaaaaattgc tataaatctt ttctcttcgc agaaccatga atacagtgat ccgtatcgtg  27540 

ctgctctctc ttcttgtaac ttttagtcag gcaggattca taccatcaat gctacatggt  27600 

gggctaatat aactttagtg ggacctcaga tattccagat cacatggtat gatagcactg  27660 

gattgcaatt ttgtgatgga agtacagtta agaatccaca gatcagacat agttgtaatg  27720 

atcaaaactt aactctgatt catgtgaaca aaacccatga aagaacatac atgggctata  27780 

ataagcagag tactcataaa gaagactata aagtcacagt tataccacct cctcctgtta  27840 

ctgtaaagcc acaaccagag ccagaatatg tgtatgttaa tatgggagag aacaaaacct  27900 

tagttgggcc tccaggaatt ccagttagtt ggtttaatca ggatggttta caattttgca  27960 

ttggggataa agtttttcat ccagaattca accacacctg tgacatgcaa aatcttacac  28020 

tgttgtttat aaatcttaca catgatggag cttatcttgg ttataatcgc cagggaactg  28080 

aaagaacttg gtatgaggtt gtagtgtcag atggttttcc aaaatcagaa gagatgaagg  28140 

tagaagacca tagtaaagaa acagaacaaa aacagactgg tcaaaaacaa agtgaccata  28200 

agcagggtgg gcaaaaagaa acaagtcaaa agaaaactaa tgacaaacaa aagccatcgc  28260 

gcaggaggcc atctaaacta aagccaaaca cacctgacac aaaactaatt acagtcacta  28320 

gtgggtcaaa cgtaacttta gttggtccag atggaaaggt cacttggtat gatgatgatt  28380 

taaaaagacc atgtgagcct gggtataagt tagggtgtaa gtgtgacaat caaaacctaa  28440 

ccctaatcaa tgtaactaaa ctttatgagg gagtttacta tggtactaat gacagaggca  28500 

acagcaaaag atacagagta aaagtaaaca ctactaattc tcaaagtgtg aaaattcagc  28560 

cgtacaccag gcctactact cctgatcaga aacacagatt tgaattgcaa attgattcta  28620 

atcaagacaa aattccatca actactgtgg caatcgtggt gggagtgatc gcgggctttg  28680 

taactctaat cattattttc atatgctaca tctgctgccg caagcgtccc aggtcataca  28740 

atcatatggt agacccacta ctcagcttct cttactgaaa ctcagtcact ctcatttcag  28800 

aaccatgaag gctttcacag cttgcgttct gattagcata gtcacactta gttcagctgc  28860 

aatgattaat gttaatgtca ctagaggtgg taaaattaca ttgaatggga cttatccaca  28920 

aactacatgg acaagatatc ataaagatgg atggaaaaat atttgtgaat ggaatgttac  28980 

tgcatacaaa tgcttcaata atggaagcat tactattact gccactgcca acattacttc  29040 

tggcacatac aaagctgaaa gctataaaaa tgaaattaaa aaattaacct ataaaaacaa  29100 

caaaaccaca tttgaagatt ctggaaatta tgagcatcaa aaattatctt tttatatgtt  29160 

gacaataatt gaactgccta caaccaaggc acccaccaca gttagtacaa ctacacagtc  29220 

aactgttaag accactactc acactacaca gctagacacc acagtgcaga ataatactgt  29280 

gttggttagg tatttgttga gggaggaaag tactactgaa cagacagagg ctacctcaag  29340 

tgcctttatc agcactgcaa atttaacttc gcttgcttgg actaatgaaa ccggagtatc  29400 

attgatgcat ggccagcctt actcaggttt ggatattcaa attacttttc tggttgtctg  29460 

tgggatcttt attcttgtgg ttcttctgta ctttgtctgc tgtaaagcca gaaagaaatc  29520 

taggaggccc atctacaggc cagtgattgg ggaacctcag ccactccaag tggatggagg  29580 

cttaaggaat cttcttttct cttttacagt atggtgatca gccatgattc ctagttcttc  29640 

ctatttaaca tcctcttctg tctcttcaac atctgtgctg cctttgcggc agtttcgcac  29700 

gcctcgcccg actgtctagg gcctttcccc acctactcct ctttgccctg ctcacctgca  29760 

cctgcgtctg cagcattgtc tgcctggtca tcaccttcct gcagctcatc gactggtgct  29820 

gcgcgcgcta caattacttc atcatagtcc cgaatacagg gacgagaacg tagccagaat  29880 

tttaaggctc atatgaccat gcagactctg ctcatactgc tatcgctctt atcccatgcc  29940 

ctcgctactg ctgattactc taaatgcaaa ttggcggaca tatggaattt cttagactgc  30000 

tatcaggaga aaattgatat gccctcctat tacttggtga ttgtgggaat agttatggtc  30060 

tgctcctgca ctttctttgc catcatgatc tacccctgtt ttgatcttgg atggaactct  30120 

gttgaggcat tcacatacac actagaaagc agttcactag cctccacgcc accacccaca  30180 

ccgcctcccc gcagaaatca gtttcccatg attcagtact tagaagagcc ccctccccga  30240 

cccccttcca ctgttagcta ctttcacata accggcggcg atgactgacc accacctgga  30300 

cctcgagatg gacggccagg cctccgagca gcgcatcctg caactgcgcg tccgtcagca  30360 

gcaggagcgt gccgccaagg agctcctcga tgccatcaac atccaccagt gcaagaaggg  30420 

catcttctgc ctggtcaaac aggcaaagat cacctacgag ctcgtgtcca acggcaaaca  30480 

gcatcgcctc acctatgaga tgccccagca gaagcagaag ttcacctgca tggtgggcgt  30540 

caaccccata gtcatcaccc agcagtcggg cgagaccaac ggctgcatcc actgctcctg  30600 

cgaaagcccc gagtgtatct actcccttct caagaccctt tgcggactcc gcgacctcct  30660 

ccccatgaac tgatgttgat taaaaaccaa aaaaaacaat cagccccttc ccctatccca  30720 

aattactcgc aaaaataaat cattggaact aatcatttaa taaagatcac ttacttgaaa  30780 

tctgaaagta tgtctctggt gtagttgttc agcagcacct cggtaccctc ctcccaactc  30840 

tggtactcca gtctccggcg ggcggcgaac tttctccaca ccttgaaagg gatgtcaaat  30900 

tcctggtcca caattttcat tgtcttccct ctcagatgtc aaagaggctc cgggtggaag  30960 

atgacttcaa ccccgtctac ccctatggct acgcgcggaa tcagaatatc cccttcctca  31020 

ctcccccctt tgtctcctcc gatggattca aaaacttccc ccctggggtc ctgtcactca  31080 

aactggctga cccaatcacc atagccaatg gtgatgtctc actcaaggtg ggagggggac  31140 

ttactttgca agaaggaagt atgactgtag accctaaggc tcccttgcaa cttgcaaaca  31200 

ataaaaaact tgagcttgtt tatgttgatc catttgaggt tagtgccaat aaacttagtt  31260 

taaaagtagg acatggatta aaaatattag atgacaaaag tgctggaggg ttgaaagatt  31320 

taattggcaa acttgtggtt ttaacaggga aaggaatagg cactgaaaat ttgcaaaata  31380 

cagatggtag cagcagagga attggtataa gtgtaagagc aagagaaggg ttaacatttg  31440 

acaatgatgg atacttggta gcatggaacc caaagtatga cacgcgcaca ctttggacaa  31500 

caccagacac atctcctaat tgcaggattg ataaggagaa ggattcaaaa ctcactttgg  31560 

tacttacaaa gtgtggaagt caaatattag ctaatgtgtc tttgattgtg gtgtcaggaa  31620 

aatatcaata catagaccac gctacaaatc caactcttaa atcatttaaa ataaaacttc  31680 

tttttgataa taaaggtgta cttctcccaa gttcaaacct tgattccaca tattggaact  31740 

ttagaagtga caatttaact gtatctgagg catataaaaa tgcagttgaa tttatgccta  31800 

atttggtagc ctacccaaaa cctaccactg gctctaaaaa atatgcaagg gatatagtct  31860 

atgggaacat atatcttgga ggtttggcat atcagccagt tgtaattaag gttactttta  31920 

atgaagaagc agatagtgct tactctataa catttgaatt tgtatggaat aaagaatatg  31980 

ccagggttga atttgaaacc acttccttta ccttctccta tattgcccaa caataaaaga  32040 

ccaataaacg tgttttttat ttcaaatttt atgtatcttt attgattttt acaccagcgc  32100 

gagtagtcaa tctcccacca ccagcccatt tcacagtgta cacggttctc tcagcacggt  32160 

ggccttaaat aaggaaatgt tctgattatt gcgggaactg gacttggggt ctataatcca  32220 

cacagtttcc tgacgagcca aacggggatc ggtgattgaa atgaagccgt cctctgaaaa  32280 

gtcatccaag cgggcctcac agtccaggtc acagtctggt ggaacgagaa gaacgcacag  32340 

attcatactc ggaaaacagg atgggtctgt gcctctccat cagcgccctc agcagtctct  32400 

gccgccgggg ctcggtgcgg ctgctgcaaa tgggatcggg atcacaagtc tctctaacta  32460 

tgatcccaac agccttcagc atcagtctcc tggtgcgtcg agcacagcac cgcatcctga  32520 

tctctgccat gttctcacag taagtgcagc acataatcac catgttattc agcagcccat  32580 

aattcagggt gctccagcca aagctcatgt tggggatgat ggaacccacg tgaccatcgt  32640 

accagatgcg gcagtatatc aggtgcctgc ccctcatgaa cacactgccc atatacatga  32700 

tctctttggg catgtttctg tttacaatct ggcggtacca ggggaagcgc tggttgaaca  32760 

tgcacccgta aatgactctc ctgaaccaca cggccagcag ggtgcctccc gcccgacact  32820 

gcagggagcc aggggatgaa cagtggcaat gcaggatcca gcgctcgtac ccgctcacca  32880 

tctgagctct taccaagtcc agggtagcgg ggcacaggca cactgacata catcttttta  32940 

aaatttttat ttcctctgtg gtgaggatca tatcccaggg gactggaaac tcttggagca  33000 

gggtaaagcc agcagcacat ggtaatccac ggacagaact tacattatga taatctgcat  33060 

gatcacaatc gggcaacagg ggatgttgat cagtcagtga agccctggtt tcatcatcag  33120 

atcgtggtaa acgggccctg cgatatggat gatggcggag cgagctggat tgaatctcgg  33180 

tttgcattgt agtggattct cttgcgtacc ttgtcgtact tctgccagca gaaatgggcc  33240 

cttgaacagc atatacccct cctgcggccg tcctttcgct gctgccgctc agtcatccaa  33300 

ctgaagtaca tccattctcg aagattctgg agaagttcct ctgcatctga tgaaataaaa  33360 

aacccgtcca tgcgaattcc cctcatcaca tcagccagga ctctgtaggc catccccatc  33420 

cagttaatgc tgccttgtct atcattcaga gggggcggtg gcaggattgg aagaaccatt  33480 

tttattccaa acggtctcga aggacgataa agtgcaagtc acgcaggtga cagcgttccc  33540 

ctccgctgtg ctggtggaaa cagacagcca ggtcaaaacc cactctattt tcaaggtgct  33600 

cgaccgtggc ttcgagcagt ggctctacgc gtacatccag cataagaatc acattaaagg  33660 

ctggccctcc atcgatttca tcaatcatca ggttacattc ctgcaccatc cccaggtaat  33720 

tctcattttt ccagccttgg attatctcta caaattgttg gtgtaaatcc actccgcaca  33780 

tgttgaaaag ctcccacagt gccccctcca ctttcataat caggcagacc ttcataatag  33840 

aaacagatcc tgctgctcca ccacctgcag cgtgttcaaa acaacaagat tcaataaggt  33900 

tctgccctcc gccctgagct cgcgcctcaa tgtcagctgc aaaaagtcac ttaagtcctg  33960 

ggccactaca gctgacaatt cagagccagg gctaagcgtg ggactggcaa gcgtgaggga  34020 

aaactttaat gctccaaagc tagcacccaa aaactgcatg ctggaataag ctctctttgt  34080 

gtctccggtg atgccttcca aaatgtgagt gataaagcgt ggtagttttt tctttaatca  34140 

tttgcgtaat agaaaagtcc tgtaaataag tcactaggac cccagggacc acaatgtggt  34200 

agcttacacc gcgtcgctga aagcatggtt agtagagatg agagtctgaa aaacagaaag  34260 

catgcgctaa actaaggtgg ctattttcac tgaaggaaaa atcactcttt ccagcagcag  34320 

ggtacccact gggtggccct tgcggacata caaaaatcgg tccgtgtgat taaaaagcag  34380 

cacagtaagt tcctgtcttc ttccggcaaa aatcacatcg gactgggtta gtatgtccct  34440 

ggcatggtag tcattcaagg ccataaatct gccctgatat ccagtaggaa ccagcacact  34500 

cacttttagg tgaagcaata ccaccccatg cggaggaatg tggaaagatt cagggcaaaa  34560 

aaaattatat ctattgctag cccttcctgg acgggagcaa tcctccagga ctatctatga  34620 

aagcatacag agattcagcc atagctcagc ccgcttacca gtagacaaag agcacagcag  34680 

tacaagcgcc aacagcagcg actgactacc cactgactta gctccctatt taaaggcacc  34740 

ttacactgac gtaatgacca aaggtctaaa aaccccgcca aaaaaacaca cacgccctgg  34800 

gtgtttttgc gaaaacactt ccgcgttctc acttcctcgt atcgatttcg tgacttgact  34860 

tccgggttcc cacgttacgt cacttttgcc cttacatgta acttagtcgt agggcgccat  34920 

cttgcccacg tccaaaatgg cttacatgtc cagttacgcc tccgcggcga ccgttagccg  34980 

tgcgtcgtga cgtcatttgc atcaacgttt ctcggccaat cagcagtagc cccgccctaa  35040 

atttaaaacc tcatttgcat attaactttt gtttactttg tggggtatat tattgatgat  35100 

g                                                                  35101 

 
           
             7  
             37474  
             DNA  
             Artificial Sequence  
             
               pMRKAd5HIV-1 gag vector, coding  
             
           
            7 

ttcttaatta acatcatcaa taatatacct tattttggat tgaagccaat atgataatga     60 

gggggtggag tttgtgacgt ggcgcggggc gtgggaacgg ggcgggtgac gtagtagtgt    120 

ggcggaagtg tgatgttgca agtgtggcgg aacacatgta agcgacggat gtggcaaaag    180 

tgacgttttt ggtgtgcgcc ggtgtacaca ggaagtgaca attttcgcgc ggttttaggc    240 

ggatgttgta gtaaatttgg gcgtaaccga gtaagatttg gccattttcg cgggaaaact    300 

gaataagagg aagtgaaatc tgaataattt tgtgttactc atagcgcgta atatttgtct    360 

agggccgcgg ggactttgac cgtttacgtg gagactcgcc caggtgtttt tctcaggtgt    420 

tttccgcgtt ccgggtcaaa gttggcgttt tattattata ggcggccgcg atccattgca    480 

tacgttgtat ccatatcata atatgtacat ttatattggc tcatgtccaa cattaccgcc    540 

atgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca    600 

tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc    660 

gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat    720 

agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt    780 

acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc    840 

cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta    900 

cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg    960 

atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt   1020 

gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac   1080 

gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa   1140 

ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga   1200 

ccgatccagc ctccgcggcc gggaacggtg cattggaacg cggattcccc gtgccaagag   1260 

tgagatctac catgggtgct agggcttctg tgctgtctgg tggtgagctg gacaagtggg   1320 

agaagatcag gctgaggcct ggtggcaaga agaagtacaa gctaaagcac attgtgtggg   1380 

cctccaggga gctggagagg tttgctgtga accctggcct gctggagacc tctgaggggt   1440 

gcaggcagat cctgggccag ctccagccct ccctgcaaac aggctctgag gagctgaggt   1500 

ccctgtacaa cacagtggct accctgtact gtgtgcacca gaagattgat gtgaaggaca   1560 

ccaaggaggc cctggagaag attgaggagg agcagaacaa gtccaagaag aaggcccagc   1620 

aggctgctgc tggcacaggc aactccagcc aggtgtccca gaactacccc attgtgcaga   1680 

acctccaggg ccagatggtg caccaggcca tctccccccg gaccctgaat gcctgggtga   1740 

aggtggtgga ggagaaggcc ttctcccctg aggtgatccc catgttctct gccctgtctg   1800 

agggtgccac cccccaggac ctgaacacca tgctgaacac agtggggggc catcaggctg   1860 

ccatgcagat gctgaaggag accatcaatg aggaggctgc tgagtgggac aggctgcatc   1920 

ctgtgcacgc tggccccatt gcccccggcc agatgaggga gcccaggggc tctgacattg   1980 

ctggcaccac ctccaccctc caggagcaga ttggctggat gaccaacaac ccccccatcc   2040 

ctgtggggga aatctacaag aggtggatca tcctgggcct gaacaagatt gtgaggatgt   2100 

actcccccac ctccatcctg gacatcaggc agggccccaa ggagcccttc agggactatg   2160 

tggacaggtt ctacaagacc ctgagggctg agcaggcctc ccaggaggtg aagaactgga   2220 

tgacagagac cctgctggtg cagaatgcca accctgactg caagaccatc ctgaaggccc   2280 

tgggccctgc tgccaccctg gaggagatga tgacagcctg ccagggggtg gggggccctg   2340 

gtcacaaggc cagggtgctg gctgaggcca tgtcccaggt gaccaactcc gccaccatca   2400 

tgatgcagag gggcaacttc aggaaccaga ggaagacagt gaagtgcttc aactgtggca   2460 

aggtgggcca cattgccaag aactgtaggg cccccaggaa gaagggctgc tggaagtgtg   2520 

gcaaggaggg ccaccagatg aaggactgca atgagaggca ggccaacttc ctgggcaaaa   2580 

tctggccctc ccacaagggc aggcctggca acttcctcca gtccaggcct gagcccacag   2640 

cccctcccga ggagtccttc aggtttgggg aggagaagac cacccccagc cagaagcagg   2700 

agcccattga caaggagctg taccccctgg cctccctgag gtccctgttt ggcaacgacc   2760 

cctcctccca gtaaaataaa gcccgggcag atctgctgtg ccttctagtt gccagccatc   2820 

tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct   2880 

ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg   2940 

gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg   3000 

ggatgcggtg ggctctatgg ccgatcggcg cgccgtactg aaatgtgtgg gcgtggctta   3060 

agggtgggaa agaatatata aggtgggggt cttatgtagt tttgtatctg ttttgcagca   3120 

gccgccgccg ccatgagcac caactcgttt gatggaagca ttgtgagctc atatttgaca   3180 

acgcgcatgc ccccatgggc cggggtgcgt cagaatgtga tgggctccag cattgatggt   3240 

cgccccgtcc tgcccgcaaa ctctactacc ttgacctacg agaccgtgtc tggaacgccg   3300 

ttggagactg cagcctccgc cgccgcttca gccgctgcag ccaccgcccg cgggattgtg   3360 

actgactttg ctttcctgag cccgcttgca aacagtgcag cttcccgttc atccgcccgc   3420 

gatgacaagt tgacggctct tttggcacaa ttggattctt tgacccggga acttaatgtc   3480 

gtttctcagc agctgttgga tctgcgccag caggtttctg ccctgaaggc ttcctcccct   3540 

cccaatgcgg tttaaaacat aaataaaaaa ccagactctg tttggatttg gatcaagcaa   3600 

gtgtcttgct gtctttattt aggggttttg cgcgcgcggt aggcccggga ccagcggtct   3660 

cggtcgttga gggtcctgtg tattttttcc aggacgtggt aaaggtgact ctggatgttc   3720 

agatacatgg gcataagccc gtctctgggg tggaggtagc accactgcag agcttcatgc   3780 

tgcggggtgg tgttgtagat gatccagtcg tagcaggagc gctgggcgtg gtgcctaaaa   3840 

atgtctttca gtagcaagct gattgccagg ggcaggccct tggtgtaagt gtttacaaag   3900 

cggttaagct gggatgggtg catacgtggg gatatgagat gcatcttgga ctgtattttt   3960 

aggttggcta tgttcccagc catatccctc cggggattca tgttgtgcag aaccaccagc   4020 

acagtgtatc cggtgcactt gggaaatttg tcatgtagct tagaaggaaa tgcgtggaag   4080 

aacttggaga cgcccttgtg acctccaaga ttttccatgc attcgtccat aatgatggca   4140 

atgggcccac gggcggcggc ctgggcgaag atatttctgg gatcactaac gtcatagttg   4200 

tgttccagga tgagatcgtc ataggccatt tttacaaagc gcgggcggag ggtgccagac   4260 

tgcggtataa tggttccatc cggcccaggg gcgtagttac cctcacagat ttgcatttcc   4320 

cacgctttga gttcagatgg ggggatcatg tctacctgcg gggcgatgaa gaaaacggtt   4380 

tccggggtag gggagatcag ctgggaagaa agcaggttcc tgagcagctg cgacttaccg   4440 

cagccggtgg gcccgtaaat cacacctatt accggctgca actggtagtt aagagagctg   4500 

cagctgccgt catccctgag caggggggcc acttcgttaa gcatgtccct gactcgcatg   4560 

ttttccctga ccaaatccgc cagaaggcgc tcgccgccca gcgatagcag ttcttgcaag   4620 

gaagcaaagt ttttcaacgg tttgagaccg tccgccgtag gcatgctttt gagcgtttga   4680 

ccaagcagtt ccaggcggtc ccacagctcg gtcacctgct ctacggcatc tcgatccagc   4740 

atatctcctc gtttcgcggg ttggggcggc tttcgctgta cggcagtagt cggtgctcgt   4800 

ccagacgggc cagggtcatg tctttccacg ggcgcagggt cctcgtcagc gtagtctggg   4860 

tcacggtgaa ggggtgcgct ccgggctgcg cgctggccag ggtgcgcttg aggctggtcc   4920 

tgctggtgct gaagcgctgc cggtcttcgc cctgcgcgtc ggccaggtag catttgacca   4980 

tggtgtcata gtccagcccc tccgcggcgt ggcccttggc gcgcagcttg cccttggagg   5040 

aggcgccgca cgaggggcag tgcagacttt tgagggcgta gagcttgggc gcgagaaata   5100 

ccgattccgg ggagtaggca tccgcgccgc aggccccgca gacggtctcg cattccacga   5160 

gccaggtgag ctctggccgt tcggggtcaa aaaccaggtt tcccccatgc tttttgatgc   5220 

gtttcttacc tctggtttcc atgagccggt gtccacgctc ggtgacgaaa aggctgtccg   5280 

tgtccccgta tacagacttg agaggcctgt cctcgagcgg tgttccgcgg tcctcctcgt   5340 

atagaaactc ggaccactct gagacaaagg ctcgcgtcca ggccagcacg aaggaggcta   5400 

agtgggaggg gtagcggtcg ttgtccacta gggggtccac tcgctccagg gtgtgaagac   5460 

acatgtcgcc ctcttcggca tcaaggaagg tgattggttt gtaggtgtag gccacgtgac   5520 

cgggtgttcc tgaagggggg ctataaaagg gggtgggggc gcgttcgtcc tcactctctt   5580 

ccgcatcgct gtctgcgagg gccagctgtt ggggtgagta ctccctctga aaagcgggca   5640 

tgacttctgc gctaagattg tcagtttcca aaaacgagga ggatttgata ttcacctggc   5700 

ccgcggtgat gcctttgagg gtggccgcat ccatctggtc agaaaagaca atctttttgt   5760 

tgtcaagctt ggtggcaaac gacccgtaga gggcgttgga cagcaacttg gcgatggagc   5820 

gcagggtttg gtttttgtcg cgatcggcgc gctccttggc cgcgatgttt agctgcacgt   5880 

attcgcgcgc aacgcaccgc cattcgggaa agacggtggt gcgctcgtcg ggcaccaggt   5940 

gcacgcgcca accgcggttg tgcagggtga caaggtcaac gctggtggct acctctccgc   6000 

gtaggcgctc gttggtccag cagaggcggc cgcccttgcg cgagcagaat ggcggtaggg   6060 

ggtctagctg cgtctcgtcc ggggggtctg cgtccacggt aaagaccccg ggcagcaggc   6120 

gcgcgtcgaa gtagtctatc ttgcatcctt gcaagtctag cgcctgctgc catgcgcggg   6180 

cggcaagcgc gcgctcgtat gggttgagtg ggggacccca tggcatgggg tgggtgagcg   6240 

cggaggcgta catgccgcaa atgtcgtaaa cgtagagggg ctctctgagt attccaagat   6300 

atgtagggta gcatcttcca ccgcggatgc tggcgcgcac gtaatcgtat agttcgtgcg   6360 

agggagcgag gaggtcggga ccgaggttgc tacgggcggg ctgctctgct cggaagacta   6420 

tctgcctgaa gatggcatgt gagttggatg atatggttgg acgctggaag acgttgaagc   6480 

tggcgtctgt gagacctacc gcgtcacgca cgaaggaggc gtaggagtcg cgcagcttgt   6540 

tgaccagctc ggcggtgacc tgcacgtcta gggcgcagta gtccagggtt tccttgatga   6600 

tgtcatactt atcctgtccc ttttttttcc acagctcgcg gttgaggaca aactcttcgc   6660 

ggtctttcca gtactcttgg atcggaaacc cgtcggcctc cgaacggtaa gagcctagca   6720 

tgtagaactg gttgacggcc tggtaggcgc agcatccctt ttctacgggt agcgcgtatg   6780 

cctgcgcggc cttccggagc gaggtgtggg tgagcgcaaa ggtgtccctg accatgactt   6840 

tgaggtactg gtatttgaag tcagtgtcgt cgcatccgcc ctgctcccag agcaaaaagt   6900 

ccgtgcgctt tttggaacgc ggatttggca gggcgaaggt gacatcgttg aagagtatct   6960 

ttcccgcgcg aggcataaag ttgcgtgtga tgcggaaggg tcccggcacc tcggaacggt   7020 

tgttaattac ctgggcggcg agcacgatct cgtcaaagcc gttgatgttg tggcccacaa   7080 

tgtaaagttc caagaagcgc gggatgccct tgatggaagg caatttttta agttcctcgt   7140 

aggtgagctc ttcaggggag ctgagcccgt gctctgaaag ggcccagtct gcaagatgag   7200 

ggttggaagc gacgaatgag ctccacaggt cacgggccat tagcatttgc aggtggtcgc   7260 

gaaaggtcct aaactggcga cctatggcca ttttttctgg ggtgatgcag tagaaggtaa   7320 

gcgggtcttg ttcccagcgg tcccatccaa ggttcgcggc taggtctcgc gcggcagtca   7380 

ctagaggctc atctccgccg aacttcatga ccagcatgaa gggcacgagc tgcttcccaa   7440 

aggcccccat ccaagtatag gtctctacat cgtaggtgac aaagagacgc tcggtgcgag   7500 

gatgcgagcc gatcgggaag aactggatct cccgccacca attggaggag tggctattga   7560 

tgtggtgaaa gtagaagtcc ctgcgacggg ccgaacactc gtgctggctt ttgtaaaaac   7620 

gtgcgcagta ctggcagcgg tgcacgggct gtacatcctg cacgaggttg acctgacgac   7680 

cgcgcacaag gaagcagagt gggaatttga gcccctcgcc tggcgggttt ggctggtggt   7740 

cttctacttc ggctgcttgt ccttgaccgt ctggctgctc gaggggagtt acggtggatc   7800 

ggaccaccac gccgcgcgag cccaaagtcc agatgtccgc gcgcggcggt cggagcttga   7860 

tgacaacatc gcgcagatgg gagctgtcca tggtctggag ctcccgcggc gtcaggtcag   7920 

gcgggagctc ctgcaggttt acctcgcata gacgggtcag ggcgcgggct agatccaggt   7980 

gatacctaat ttccaggggc tggttggtgg cggcgtcgat ggcttgcaag aggccgcatc   8040 

cccgcggcgc gactacggta ccgcgcggcg ggcggtgggc cgcgggggtg tccttggatg   8100 

atgcatctaa aagcggtgac gcgggcgagc ccccggaggt agggggggct ccggacccgc   8160 

cgggagaggg ggcaggggca cgtcggcgcc gcgcgcgggc aggagctggt gctgcgcgcg   8220 

taggttgctg gcgaacgcga cgacgcggcg gttgatctcc tgaatctggc gcctctgcgt   8280 

gaagacgacg ggcccggtga gcttgaacct gaaagagagt tcgacagaat caatttcggt   8340 

gtcgttgacg gcggcctggc gcaaaatctc ctgcacgtct cctgagttgt cttgataggc   8400 

gatctcggcc atgaactgct cgatctcttc ctcctggaga tctccgcgtc cggctcgctc   8460 

cacggtggcg gcgaggtcgt tggaaatgcg ggccatgagc tgcgagaagg cgttgaggcc   8520 

tccctcgttc cagacgcggc tgtagaccac gcccccttcg gcatcgcggg cgcgcatgac   8580 

cacctgcgcg agattgagct ccacgtgccg ggcgaagacg gcgtagtttc gcaggcgctg   8640 

aaagaggtag ttgagggtgg tggcggtgtg ttctgccacg aagaagtaca taacccagcg   8700 

tcgcaacgtg gattcgttga tatcccccaa ggcctcaagg cgctccatgg cctcgtagaa   8760 

gtccacggcg aagttgaaaa actgggagtt gcgcgccgac acggttaact cctcctccag   8820 

aagacggatg agctcggcga cagtgtcgcg cacctcgcgc tcaaaggcta caggggcctc   8880 

ttcttcttct tcaatctcct cttccataag ggcctcccct tcttcttctt ctggcggcgg   8940 

tgggggaggg gggacacggc ggcgacgacg gcgcaccggg aggcggtcga caaagcgctc   9000 

gatcatctcc ccgcggcgac ggcgcatggt ctcggtgacg gcgcggccgt tctcgcgggg   9060 

gcgcagttgg aagacgccgc ccgtcatgtc ccggttatgg gttggcgggg ggctgccatg   9120 

cggcagggat acggcgctaa cgatgcatct caacaattgt tgtgtaggta ctccgccgcc   9180 

gagggacctg agcgagtccg catcgaccgg atcggaaaac ctctcgagaa aggcgtctaa   9240 

ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc ggcagcgggc ggcggtcggg   9300 

gttgtttctg gcggaggtgc tgctgatgat gtaattaaag taggcggtct tgagacggcg   9360 

gatggtcgac agaagcacca tgtccttggg tccggcctgc tgaatgcgca ggcggtcggc   9420 

catgccccag gcttcgtttt gacatcggcg caggtctttg tagtagtctt gcatgagcct   9480 

ttctaccggc acttcttctt ctccttcctc ttgtcctgca tctcttgcat ctatcgctgc   9540 

ggcggcggcg gagtttggcc gtaggtggcg ccctcttcct cccatgcgtg tgaccccgaa   9600 

gcccctcatc ggctgaagca gggctaggtc ggcgacaacg cgctcggcta atatggcctg   9660 

ctgcacctgc gtgagggtag actggaagtc atccatgtcc acaaagcggt ggtatgcgcc   9720 

cgtgttgatg gtgtaagtgc agttggccat aacggaccag ttaacggtct ggtgacccgg   9780 

ctgcgagagc tcggtgtacc tgagacgcga gtaagccctc gagtcaaata cgtagtcgtt   9840 

gcaagtccgc accaggtact ggtatcccac caaaaagtgc ggcggcggct ggcggtagag   9900 

gggccagcgt agggtggccg gggctccggg ggcgagatct tccaacataa ggcgatgata   9960 

tccgtagatg tacctggaca tccaggtgat gccggcggcg gtggtggagg cgcgcggaaa  10020 

gtcgcggacg cggttccaga tgttgcgcag cggcaaaaag tgctccatgg tcgggacgct  10080 

ctggccggtc aggcgcgcgc aatcgttgac gctctagacc gtgcaaaagg agagcctgta  10140 

agcgggcact cttccgtggt ctggtggata aattcgcaag ggtatcatgg cggacgaccg  10200 

gggttcgagc cccgtatccg gccgtccgcc gtgatccatg cggttaccgc ccgcgtgtcg  10260 

aacccaggtg tgcgacgtca gacaacgggg gagtgctcct tttggcttcc ttccaggcgc  10320 

ggcggctgct gcgctagctt ttttggccac tggccgcgcg cagcgtaagc ggttaggctg  10380 

gaaagcgaaa gcattaagtg gctcgctccc tgtagccgga gggttatttt ccaagggttg  10440 

agtcgcggga cccccggttc gagtctcgga ccggccggac tgcggcgaac gggggtttgc  10500 

ctccccgtca tgcaagaccc cgcttgcaaa ttcctccgga aacagggacg agcccctttt  10560 

ttgcttttcc cagatgcatc cggtgctgcg gcagatgcgc ccccctcctc agcagcggca  10620 

agagcaagag cagcggcaga catgcagggc accctcccct cctcctaccg cgtcaggagg  10680 

ggcgacatcc gcggttgacg cggcagcaga tggtgattac gaacccccgc ggcgccgggc  10740 

ccggcactac ctggacttgg aggagggcga gggcctggcg cggctaggag cgccctctcc  10800 

tgagcggcac ccaagggtgc agctgaagcg tgatacgcgt gaggcgtacg tgccgcggca  10860 

gaacctgttt cgcgaccgcg agggagagga gcccgaggag atgcgggatc gaaagttcca  10920 

cgcagggcgc gagctgcggc atggcctgaa tcgcgagcgg ttgctgcgcg aggaggactt  10980 

tgagcccgac gcgcgaaccg ggattagtcc cgcgcgcgca cacgtggcgg ccgccgacct  11040 

ggtaaccgca tacgagcaga cggtgaacca ggagattaac tttcaaaaaa gctttaacaa  11100 

ccacgtgcgt acgcttgtgg cgcgcgagga ggtggctata ggactgatgc atctgtggga  11160 

ctttgtaagc gcgctggagc aaaacccaaa tagcaagccg ctcatggcgc agctgttcct  11220 

tatagtgcag cacagcaggg acaacgaggc attcagggat gcgctgctaa acatagtaga  11280 

gcccgagggc cgctggctgc tcgatttgat aaacatcctg cagagcatag tggtgcagga  11340 

gcgcagcttg agcctggctg acaaggtggc cgccatcaac tattccatgc ttagcctggg  11400 

caagttttac gcccgcaaga tataccatac cccttacgtt cccatagaca aggaggtaaa  11460 

gatcgagggg ttctacatgc gcatggcgct gaaggtgctt accttgagcg acgacctggg  11520 

cgtttatcgc aacgagcgca tccacaaggc cgtgagcgtg agccggcggc gcgagctcag  11580 

cgaccgcgag ctgatgcaca gcctgcaaag ggccctggct ggcacgggca gcggcgatag  11640 

agaggccgag tcctactttg acgcgggcgc tgacctgcgc tgggccccaa gccgacgcgc  11700 

cctggaggca gctggggccg gacctgggct ggcggtggca cccgcgcgcg ctggcaacgt  11760 

cggcggcgtg gaggaatatg acgaggacga tgagtacgag ccagaggacg gcgagtacta  11820 

agcggtgatg tttctgatca gatgatgcaa gacgcaacgg acccggcggt gcgggcggcg  11880 

ctgcagagcc agccgtccgg ccttaactcc acggacgact ggcgccaggt catggaccgc  11940 

atcatgtcgc tgactgcgcg caatcctgac gcgttccggc agcagccgca ggccaaccgg  12000 

ctctccgcaa ttctggaagc ggtggtcccg gcgcgcgcaa accccacgca cgagaaggtg  12060 

ctggcgatcg taaacgcgct ggccgaaaac agggccatcc ggcccgacga ggccggcctg  12120 

gtctacgacg cgctgcttca gcgcgtggct cgttacaaca gcggcaacgt gcagaccaac  12180 

ctggaccggc tggtggggga tgtgcgcgag gccgtggcgc agcgtgagcg cgcgcagcag  12240 

cagggcaacc tgggctccat ggttgcacta aacgccttcc tgagtacaca gcccgccaac  12300 

gtgccgcggg gacaggagga ctacaccaac tttgtgagcg cactgcggct aatggtgact  12360 

gagacaccgc aaagtgaggt gtaccagtct gggccagact attttttcca gaccagtaga  12420 

caaggcctgc agaccgtaaa cctgagccag gctttcaaaa acttgcaggg gctgtggggg  12480 

gtgcgggctc ccacaggcga ccgcgcgacc gtgtctagct tgctgacgcc caactcgcgc  12540 

ctgttgctgc tgctaatagc gcccttcacg gacagtggca gcgtgtcccg ggacacatac  12600 

ctaggtcact tgctgacact gtaccgcgag gccataggtc aggcgcatgt ggacgagcat  12660 

actttccagg agattacaag tgtcagccgc gcgctggggc aggaggacac gggcagcctg  12720 

gaggcaaccc taaactacct gctgaccaac cggcggcaga agatcccctc gttgcacagt  12780 

ttaaacagcg aggaggagcg cattttgcgc tacgtgcagc agagcgtgag ccttaacctg  12840 

atgcgcgacg gggtaacgcc cagcgtggcg ctggacatga ccgcgcgcaa catggaaccg  12900 

ggcatgtatg cctcaaaccg gccgtttatc aaccgcctaa tggactactt gcatcgcgcg  12960 

gccgccgtga accccgagta tttcaccaat gccatcttga acccgcactg gctaccgccc  13020 

cctggtttct acaccggggg attcgaggtg cccgagggta acgatggatt cctctgggac  13080 

gacatagacg acagcgtgtt ttccccgcaa ccgcagaccc tgctagagtt gcaacagcgc  13140 

gagcaggcag aggcggcgct gcgaaaggaa agcttccgca ggccaagcag cttgtccgat  13200 

ctaggcgctg cggccccgcg gtcagatgct agtagcccat ttccaagctt gatagggtct  13260 

cttaccagca ctcgcaccac ccgcccgcgc ctgctgggcg aggaggagta cctaaacaac  13320 

tcgctgctgc agccgcagcg cgaaaaaaac ctgcctccgg catttcccaa caacgggata  13380 

gagagcctag tggacaagat gagtagatgg aagacgtacg cgcaggagca cagggacgtg  13440 

ccaggcccgc gcccgcccac ccgtcgtcaa aggcacgacc gtcagcgggg tctggtgtgg  13500 

gaggacgatg actcggcaga cgacagcagc gtcctggatt tgggagggag tggcaacccg  13560 

tttgcgcacc ttcgccccag gctggggaga atgttttaaa aaaaaaaaaa gcatgatgca  13620 

aaataaaaaa ctcaccaagg ccatggcacc gagcgttggt tttcttgtat tccccttagt  13680 

atgcggcgcg cggcgatgta tgaggaaggt cctcctccct cctacgagag tgtggtgagc  13740 

gcggcgccag tggcggcggc gctgggttct cccttcgatg ctcccctgga cccgccgttt  13800 

gtgcctccgc ggtacctgcg gcctaccggg gggagaaaca gcatccgtta ctctgagttg  13860 

gcacccctat tcgacaccac ccgtgtgtac ctggtggaca acaagtcaac ggatgtggca  13920 

tccctgaact accagaacga ccacagcaac tttctgacca cggtcattca aaacaatgac  13980 

tacagcccgg gggaggcaag cacacagacc atcaatcttg acgaccggtc gcactggggc  14040 

ggcgacctga aaaccatcct gcataccaac atgccaaatg tgaacgagtt catgtttacc  14100 

aataagttta aggcgcgggt gatggtgtcg cgcttgccta ctaaggacaa tcaggtggag  14160 

ctgaaatacg agtgggtgga gttcacgctg cccgagggca actactccga gaccatgacc  14220 

atagacctta tgaacaacgc gatcgtggag cactacttga aagtgggcag acagaacggg  14280 

gttctggaaa gcgacatcgg ggtaaagttt gacacccgca acttcagact ggggtttgac  14340 

cccgtcactg gtcttgtcat gcctggggta tatacaaacg aagccttcca tccagacatc  14400 

attttgctgc caggatgcgg ggtggacttc acccacagcc gcctgagcaa cttgttgggc  14460 

atccgcaagc ggcaaccctt ccaggagggc tttaggatca cctacgatga tctggagggt  14520 

ggtaacattc ccgcactgtt ggatgtggac gcctaccagg cgagcttgaa agatgacacc  14580 

gaacagggcg ggggtggcgc aggcggcagc aacagcagtg gcagcggcgc ggaagagaac  14640 

tccaacgcgg cagccgcggc aatgcagccg gtggaggaca tgaacgatca tgccattcgc  14700 

ggcgacacct ttgccacacg ggctgaggag aagcgcgctg aggccgaagc agcggccgaa  14760 

gctgccgccc ccgctgcgca acccgaggtc gagaagcctc agaagaaacc ggtgatcaaa  14820 

cccctgacag aggacagcaa gaaacgcagt tacaacctaa taagcaatga cagcaccttc  14880 

acccagtacc gcagctggta ccttgcatac aactacggcg accctcagac cggaatccgc  14940 

tcatggaccc tgctttgcac tcctgacgta acctgcggct cggagcaggt ctactggtcg  15000 

ttgccagaca tgatgcaaga ccccgtgacc ttccgctcca cgcgccagat cagcaacttt  15060 

ccggtggtgg gcgccgagct gttgcccgtg cactccaaga gcttctacaa cgaccaggcc  15120 

gtctactccc aactcatccg ccagtttacc tctctgaccc acgtgttcaa tcgctttccc  15180 

gagaaccaga ttttggcgcg cccgccagcc cccaccatca ccaccgtcag tgaaaacgtt  15240 

cctgctctca cagatcacgg gacgctaccg ctgcgcaaca gcatcggagg agtccagcga  15300 

gtgaccatta ctgacgccag acgccgcacc tgcccctacg tttacaaggc cctgggcata  15360 

gtctcgccgc gcgtcctatc gagccgcact ttttgagcaa gcatgtccat ccttatatcg  15420 

cccagcaata acacaggctg gggcctgcgc ttcccaagca agatgtttgg cggggccaag  15480 

aagcgctccg accaacaccc agtgcgcgtg cgcgggcact accgcgcgcc ctggggcgcg  15540 

cacaaacgcg gccgcactgg gcgcaccacc gtcgatgacg ccatcgacgc ggtggtggag  15600 

gaggcgcgca actacacgcc cacgccgcca ccagtgtcca cagtggacgc ggccattcag  15660 

accgtggtgc gcggagcccg gcgctatgct aaaatgaaga gacggcggag gcgcgtagca  15720 

cgtcgccacc gccgccgacc cggcactgcc gcccaacgcg cggcggcggc cctgcttaac  15780 

cgcgcacgtc gcaccggccg acgggcggcc atgcgggccg ctcgaaggct ggccgcgggt  15840 

attgtcactg tgccccccag gtccaggcga cgagcggccg ccgcagcagc cgcggccatt  15900 

agtgctatga ctcagggtcg caggggcaac gtgtattggg tgcgcgactc ggttagcggc  15960 

ctgcgcgtgc ccgtgcgcac ccgccccccg cgcaactaga ttgcaagaaa aaactactta  16020 

gactcgtact gttgtatgta tccagcggcg gcggcgcgca acgaagctat gtccaagcgc  16080 

aaaatcaaag aagagatgct ccaggtcatc gcgccggaga tctatggccc cccgaagaag  16140 

gaagagcagg attacaagcc ccgaaagcta aagcgggtca aaaagaaaaa gaaagatgat  16200 

gatgatgaac ttgacgacga ggtggaactg ctgcacgcta ccgcgcccag gcgacgggta  16260 

cagtggaaag gtcgacgcgt aaaacgtgtt ttgcgacccg gcaccaccgt agtctttacg  16320 

cccggtgagc gctccacccg cacctacaag cgcgtgtatg atgaggtgta cggcgacgag  16380 

gacctgcttg agcaggccaa cgagcgcctc ggggagtttg cctacggaaa gcggcataag  16440 

gacatgctgg cgttgccgct ggacgagggc aacccaacac ctagcctaaa gcccgtaaca  16500 

ctgcagcagg tgctgcccgc gcttgcaccg tccgaagaaa agcgcggcct aaagcgcgag  16560 

tctggtgact tggcacccac cgtgcagctg atggtaccca agcgccagcg actggaagat  16620 

gtcttggaaa aaatgaccgt ggaacctggg ctggagcccg aggtccgcgt gcggccaatc  16680 

aagcaggtgg cgccgggact gggcgtgcag accgtggacg ttcagatacc cactaccagt  16740 

agcaccagta ttgccaccgc cacagagggc atggagacac aaacgtcccc ggttgcctca  16800 

gcggtggcgg atgccgcggt gcaggcggtc gctgcggccg cgtccaagac ctctacggag  16860 

gtgcaaacgg acccgtggat gtttcgcgtt tcagcccccc ggcgcccgcg ccgttcgagg  16920 

aagtacggcg ccgccagcgc gctactgccc gaatatgccc tacatccttc cattgcgcct  16980 

acccccggct atcgtggcta cacctaccgc cccagaagac gagcaactac ccgacgccga  17040 

accaccactg gaacccgccg ccgccgtcgc cgtcgccagc ccgtgctggc cccgatttcc  17100 

gtgcgcaggg tggctcgcga aggaggcagg accctggtgc tgccaacagc gcgctaccac  17160 

cccagcatcg tttaaaagcc ggtctttgtg gttcttgcag atatggccct cacctgccgc  17220 

ctccgtttcc cggtgccggg attccgagga agaatgcacc gtaggagggg catggccggc  17280 

cacggcctga cgggcggcat gcgtcgtgcg caccaccggc ggcggcgcgc gtcgcaccgt  17340 

cgcatgcgcg gcggtatcct gcccctcctt attccactga tcgccgcggc gattggcgcc  17400 

gtgcccggaa ttgcatccgt ggccttgcag gcgcagagac actgattaaa aacaagttgc  17460 

atgtggaaaa atcaaaataa aaagtctgga ctctcacgct cgcttggtcc tgtaactatt  17520 

ttgtagaatg gaagacatca actttgcgtc tctggccccg cgacacggct cgcgcccgtt  17580 

catgggaaac tggcaagata tcggcaccag caatatgagc ggtggcgcct tcagctgggg  17640 

ctcgctgtgg agcggcatta aaaatttcgg ttccaccgtt aagaactatg gcagcaaggc  17700 

ctggaacagc agcacaggcc agatgctgag ggataagttg aaagagcaaa atttccaaca  17760 

aaaggtggta gatggcctgg cctctggcat tagcggggtg gtggacctgg ccaaccaggc  17820 

agtgcaaaat aagattaaca gtaagcttga tccccgccct cccgtagagg agcctccacc  17880 

ggccgtggag acagtgtctc cagaggggcg tggcgaaaag cgtccgcgcc ccgacaggga  17940 

agaaactctg gtgacgcaaa tagacgagcc tccctcgtac gaggaggcac taaagcaagg  18000 

cctgcccacc acccgtccca tcgcgcccat ggctaccgga gtgctgggcc agcacacacc  18060 

cgtaacgctg gacctgcctc cccccgccga cacccagcag aaacctgtgc tgccaggccc  18120 

gaccgccgtt gttgtaaccc gtcctagccg cgcgtccctg cgccgcgccg ccagcggtcc  18180 

gcgatcgttg cggcccgtag ccagtggcaa ctggcaaagc acactgaaca gcatcgtggg  18240 

tctgggggtg caatccctga agcgccgacg atgcttctga tagctaacgt gtcgtatgtg  18300 

tgtcatgtat gcgtccatgt cgccgccaga ggagctgctg agccgccgcg cgcccgcttt  18360 

ccaagatggc taccccttcg atgatgccgc agtggtctta catgcacatc tcgggccagg  18420 

acgcctcgga gtacctgagc cccgggctgg tgcagtttgc ccgcgccacc gagacgtact  18480 

tcagcctgaa taacaagttt agaaacccca cggtggcgcc tacgcacgac gtgaccacag  18540 

accggtccca gcgtttgacg ctgcggttca tccctgtgga ccgtgaggat actgcgtact  18600 

cgtacaaggc gcggttcacc ctagctgtgg gtgataaccg tgtgctggac atggcttcca  18660 

cgtactttga catccgcggc gtgctggaca ggggccctac ttttaagccc tactctggca  18720 

ctgcctacaa cgccctggct cccaagggtg ccccaaatcc ttgcgaatgg gatgaagctg  18780 

ctactgctct tgaaataaac ctagaagaag aggacgatga caacgaagac gaagtagacg  18840 

agcaagctga gcagcaaaaa actcacgtat ttgggcaggc gccttattct ggtataaata  18900 

ttacaaagga gggtattcaa ataggtgtcg aaggtcaaac acctaaatat gccgataaaa  18960 

catttcaacc tgaacctcaa ataggagaat ctcagtggta cgaaacagaa attaatcatg  19020 

cagctgggag agtcctaaaa aagactaccc caatgaaacc atgttacggt tcatatgcaa  19080 

aacccacaaa tgaaaatgga gggcaaggca ttcttgtaaa gcaacaaaat ggaaagctag  19140 

aaagtcaagt ggaaatgcaa tttttctcaa ctactgaggc agccgcaggc aatggtgata  19200 

acttgactcc taaagtggta ttgtacagtg aagatgtaga tatagaaacc ccagacactc  19260 

atatttctta catgcccact attaaggaag gtaactcacg agaactaatg ggccaacaat  19320 

ctatgcccaa caggcctaat tacattgctt ttagggacaa ttttattggt ctaatgtatt  19380 

acaacagcac gggtaatatg ggtgttctgg cgggccaagc atcgcagttg aatgctgttg  19440 

tagatttgca agacagaaac acagagcttt cataccagct tttgcttgat tccattggtg  19500 

atagaaccag gtacttttct atgtggaatc aggctgttga cagctatgat ccagatgtta  19560 

gaattattga aaatcatgga actgaagatg aacttccaaa ttactgcttt ccactgggag  19620 

gtgtgattaa tacagagact cttaccaagg taaaacctaa aacaggtcag gaaaatggat  19680 

gggaaaaaga tgctacagaa ttttcagata aaaatgaaat aagagttgga aataattttg  19740 

ccatggaaat caatctaaat gccaacctgt ggagaaattt cctgtactcc aacatagcgc  19800 

tgtatttgcc cgacaagcta aagtacagtc cttccaacgt aaaaatttct gataacccaa  19860 

acacctacga ctacatgaac aagcgagtgg tggctcccgg gctagtggac tgctacatta  19920 

accttggagc acgctggtcc cttgactata tggacaacgt caacccattt aaccaccacc  19980 

gcaatgctgg cctgcgctac cgctcaatgt tgctgggcaa tggtcgctat gtgcccttcc  20040 

acatccaggt gcctcagaag ttctttgcca ttaaaaacct ccttctcctg ccgggctcat  20100 

acacctacga gtggaacttc aggaaggatg ttaacatggt tctgcagagc tccctaggaa  20160 

atgacctaag ggttgacgga gccagcatta agtttgatag catttgcctt tacgccacct  20220 

tcttccccat ggcccacaac accgcctcca cgcttgaggc catgcttaga aacgacacca  20280 

acgaccagtc ctttaacgac tatctctccg ccgccaacat gctctaccct atacccgcca  20340 

acgctaccaa cgtgcccata tccatcccct cccgcaactg ggcggctttc cgcggctggg  20400 

ccttcacgcg ccttaagact aaggaaaccc catcactggg ctcgggctac gacccttatt  20460 

acacctactc tggctctata ccctacctag atggaacctt ttacctcaac cacaccttta  20520 

agaaggtggc cattaccttt gactcttctg tcagctggcc tggcaatgac cgcctgctta  20580 

cccccaacga gtttgaaatt aagcgctcag ttgacgggga gggttacaac gttgcccagt  20640 

gtaacatgac caaagactgg ttcctggtac aaatgctagc taactataac attggctacc  20700 

agggcttcta tatcccagag agctacaagg accgcatgta ctccttcttt agaaacttcc  20760 

agcccatgag ccgtcaggtg gtggatgata ctaaatacaa ggactaccaa caggtgggca  20820 

tcctacacca acacaacaac tctggatttg ttggctacct tgcccccacc atgcgcgaag  20880 

gacaggccta ccctgctaac ttcccctatc cgcttatagg caagaccgca gttgacagca  20940 

ttacccagaa aaagtttctt tgcgatcgca ccctttggcg catcccattc tccagtaact  21000 

ttatgtccat gggcgcactc acagacctgg gccaaaacct tctctacgcc aactccgccc  21060 

acgcgctaga catgactttt gaggtggatc ccatggacga gcccaccctt ctttatgttt  21120 

tgtttgaagt ctttgacgtg gtccgtgtgc accagccgca ccgcggcgtc atcgaaaccg  21180 

tgtacctgcg cacgcccttc tcggccggca acgccacaac ataaagaagc aagcaacatc  21240 

aacaacagct gccgccatgg gctccagtga gcaggaactg aaagccattg tcaaagatct  21300 

tggttgtggg ccatattttt tgggcaccta tgacaagcgc tttccaggct ttgtttctcc  21360 

acacaagctc gcctgcgcca tagtcaatac ggccggtcgc gagactgggg gcgtacactg  21420 

gatggccttt gcctggaacc cgcactcaaa aacatgctac ctctttgagc cctttggctt  21480 

ttctgaccag cgactcaagc aggtttacca gtttgagtac gagtcactcc tgcgccgtag  21540 

cgccattgct tcttcccccg accgctgtat aacgctggaa aagtccaccc aaagcgtaca  21600 

ggggcccaac tcggccgcct gtggactatt ctgctgcatg tttctccacg cctttgccaa  21660 

ctggccccaa actcccatgg atcacaaccc caccatgaac cttattaccg gggtacccaa  21720 

ctccatgctc aacagtcccc aggtacagcc caccctgcgt cgcaaccagg aacagctcta  21780 

cagcttcctg gagcgccact cgccctactt ccgcagccac agtgcgcaga ttaggagcgc  21840 

cacttctttt tgtcacttga aaaacatgta aaaataatgt actagagaca ctttcaataa  21900 

aggcaaatgc ttttatttgt acactctcgg gtgattattt acccccaccc ttgccgtctg  21960 

cgccgtttaa aaatcaaagg ggttctgccg cgcatcgcta tgcgccactg gcagggacac  22020 

gttgcgatac tggtgtttag tgctccactt aaactcaggc acaaccatcc gcggcagctc  22080 

ggtgaagttt tcactccaca ggctgcgcac catcaccaac gcgtttagca ggtcgggcgc  22140 

cgatatcttg aagtcgcagt tggggcctcc gccctgcgcg cgcgagttgc gatacacagg  22200 

gttgcagcac tggaacacta tcagcgccgg gtggtgcacg ctggccagca cgctcttgtc  22260 

ggagatcaga tccgcgtcca ggtcctccgc gttgctcagg gcgaacggag tcaactttgg  22320 

tagctgcctt cccaaaaagg gcgcgtgccc aggctttgag ttgcactcgc accgtagtgg  22380 

catcaaaagg tgaccgtgcc cggtctgggc gttaggatac agcgcctgca taaaagcctt  22440 

gatctgctta aaagccacct gagcctttgc gccttcagag aagaacatgc cgcaagactt  22500 

gccggaaaac tgattggccg gacaggccgc gtcgtgcacg cagcaccttg cgtcggtgtt  22560 

ggagatctgc accacatttc ggccccaccg gttcttcacg atcttggcct tgctagactg  22620 

ctccttcagc gcgcgctgcc cgttttcgct cgtcacatcc atttcaatca cgtgctcctt  22680 

atttatcata atgcttccgt gtagacactt aagctcgcct tcgatctcag cgcagcggtg  22740 

cagccacaac gcgcagcccg tgggctcgtg atgcttgtag gtcacctctg caaacgactg  22800 

caggtacgcc tgcaggaatc gccccatcat cgtcacaaag gtcttgttgc tggtgaaggt  22860 

cagctgcaac ccgcggtgct cctcgttcag ccaggtcttg catacggccg ccagagcttc  22920 

cacttggtca ggcagtagtt tgaagttcgc ctttagatcg ttatccacgt ggtacttgtc  22980 

catcagcgcg cgcgcagcct ccatgccctt ctcccacgca gacacgatcg gcacactcag  23040 

cgggttcatc accgtaattt cactttccgc ttcgctgggc tcttcctctt cctcttgcgt  23100 

ccgcatacca cgcgccactg ggtcgtcttc attcagccgc cgcactgtgc gcttacctcc  23160 

tttgccatgc ttgattagca ccggtgggtt gctgaaaccc accatttgta gcgccacatc  23220 

ttctctttct tcctcgctgt ccacgattac ctctggtgat ggcgggcgct cgggcttggg  23280 

agaagggcgc ttctttttct tcttgggcgc aatggccaaa tccgccgccg aggtcgatgg  23340 

ccgcgggctg ggtgtgcgcg gcaccagcgc gtcttgtgat gagtcttcct cgtcctcgga  23400 

ctcgatacgc cgcctcatcc gcttttttgg gggcgcccgg ggaggcggcg gcgacgggga  23460 

cggggacgac acgtcctcca tggttggggg acgtcgcgcc gcaccgcgtc cgcgctcggg  23520 

ggtggtttcg cgctgctcct cttcccgact ggccatttcc ttctcctata ggcagaaaaa  23580 

gatcatggag tcagtcgaga agaaggacag cctaaccgcc ccctctgagt tcgccaccac  23640 

cgcctccacc gatgccgcca acgcgcctac caccttcccc gtcgaggcac ccccgcttga  23700 

ggaggaggaa gtgattatcg agcaggaccc aggttttgta agcgaagacg acgaggaccg  23760 

ctcagtacca acagaggata aaaagcaaga ccaggacaac gcagaggcaa acgaggaaca  23820 

agtcgggcgg ggggacgaaa ggcatggcga ctacctagat gtgggagacg acgtgctgtt  23880 

gaagcatctg cagcgccagt gcgccattat ctgcgacgcg ttgcaagagc gcagcgatgt  23940 

gcccctcgcc atagcggatg tcagccttgc ctacgaacgc cacctattct caccgcgcgt  24000 

accccccaaa cgccaagaaa acggcacatg cgagcccaac ccgcgcctca acttctaccc  24060 

cgtatttgcc gtgccagagg tgcttgccac ctatcacatc tttttccaaa actgcaagat  24120 

acccctatcc tgccgtgcca accgcagccg agcggacaag cagctggcct tgcggcaggg  24180 

cgctgtcata cctgatatcg cctcgctcaa cgaagtgcca aaaatctttg agggtcttgg  24240 

acgcgacgag aagcgcgcgg caaacgctct gcaacaggaa aacagcgaaa atgaaagtca  24300 

ctctggagtg ttggtggaac tcgagggtga caacgcgcgc ctagccgtac taaaacgcag  24360 

catcgaggtc acccactttg cctacccggc acttaaccta ccccccaagg tcatgagcac  24420 

agtcatgagt gagctgatcg tgcgccgtgc gcagcccctg gagagggatg caaatttgca  24480 

agaacaaaca gaggagggcc tacccgcagt tggcgacgag cagctagcgc gctggcttca  24540 

aacgcgcgag cctgccgact tggaggagcg acgcaaacta atgatggccg cagtgctcgt  24600 

taccgtggag cttgagtgca tgcagcggtt ctttgctgac ccggagatgc agcgcaagct  24660 

agaggaaaca ttgcactaca cctttcgaca gggctacgta cgccaggcct gcaagatctc  24720 

caacgtggag ctctgcaacc tggtctccta ccttggaatt ttgcacgaaa accgccttgg  24780 

gcaaaacgtg cttcattcca cgctcaaggg cgaggcgcgc cgcgactacg tccgcgactg  24840 

cgtttactta tttctatgct acacctggca gacggccatg ggcgtttggc agcagtgctt  24900 

ggaggagtgc aacctcaagg agctgcagaa actgctaaag caaaacttga aggacctatg  24960 

gacggccttc aacgagcgct ccgtggccgc gcacctggcg gacatcattt tccccgaacg  25020 

cctgcttaaa accctgcaac agggtctgcc agacttcacc agtcaaagca tgttgcagaa  25080 

ctttaggaac tttatcctag agcgctcagg aatcttgccc gccacctgct gtgcacttcc  25140 

tagcgacttt gtgcccatta agtaccgcga atgccctccg ccgctttggg gccactgcta  25200 

ccttctgcag ctagccaact accttgccta ccactctgac ataatggaag acgtgagcgg  25260 

tgacggtcta ctggagtgtc actgtcgctg caacctatgc accccgcacc gctccctggt  25320 

ttgcaattcg cagctgctta acgaaagtca aattatcggt acctttgagc tgcagggtcc  25380 

ctcgcctgac gaaaagtccg cggctccggg gttgaaactc actccggggc tgtggacgtc  25440 

ggcttacctt cgcaaatttg tacctgagga ctaccacgcc cacgagatta ggttctacga  25500 

agaccaatcc cgcccgccta atgcggagct taccgcctgc gtcattaccc agggccacat  25560 

tcttggccaa ttgcaagcca tcaacaaagc ccgccaagag tttctgctac gaaagggacg  25620 

gggggtttac ttggaccccc agtccggcga ggagctcaac ccaatccccc cgccgccgca  25680 

gccctatcag cagcagccgc gggcccttgc ttcccaggat ggcacccaaa aagaagctgc  25740 

agctgccgcc gccacccacg gacgaggagg aatactggga cagtcaggca gaggaggttt  25800 

tggacgagga ggaggaggac atgatggaag actgggagag cctagacgag gaagcttccg  25860 

aggtcgaaga ggtgtcagac gaaacaccgt caccctcggt cgcattcccc tcgccggcgc  25920 

cccagaaatc ggcaaccggt tccagcatgg ctacaacctc cgctcctcag gcgccgccgg  25980 

cactgcccgt tcgccgaccc aaccgtagat gggacaccac tggaaccagg gccggtaagt  26040 

ccaagcagcc gccgccgtta gcccaagagc aacaacagcg ccaaggctac cgctcatggc  26100 

gcgggcacaa gaacgccata gttgcttgct tgcaagactg tgggggcaac atctccttcg  26160 

cccgccgctt tcttctctac catcacggcg tggccttccc ccgtaacatc ctgcattact  26220 

accgtcatct ctacagccca tactgcaccg gcggcagcgg cagcaacagc agcggccaca  26280 

cagaagcaaa ggcgaccgga tagcaagact ctgacaaagc ccaagaaatc cacagcggcg  26340 

gcagcagcag gaggaggagc gctgcgtctg gcgcccaacg aacccgtatc gacccgcgag  26400 

cttagaaaca ggatttttcc cactctgtat gctatatttc aacagagcag gggccaagaa  26460 

caagagctga aaataaaaaa caggtctctg cgatccctca cccgcagctg cctgtatcac  26520 

aaaagcgaag atcagcttcg gcgcacgctg gaagacgcgg aggctctctt cagtaaatac  26580 

tgcgcgctga ctcttaagga ctagtttcgc gccctttctc aaatttaagc gcgaaaacta  26640 

cgtcatctcc agcggccaca cccggcgcca gcacctgttg tcagcgccat tatgagcaag  26700 

gaaattccca cgccctacat gtggagttac cagccacaaa tgggacttgc ggctggagct  26760 

gcccaagact actcaacccg aataaactac atgagcgcgg gaccccacat gatatcccgg  26820 

gtcaacggaa tacgcgccca ccgaaaccga attctcctgg aacaggcggc tattaccacc  26880 

acacctcgta ataaccttaa tccccgtagt tggcccgctg ccctggtgta ccaggaaagt  26940 

cccgctccca ccactgtggt acttcccaga gacgcccagg ccgaagttca gatgactaac  27000 

tcaggggcgc agcttgcggg cggctttcgt cacagggtgc ggtcgcccgg gcagggtata  27060 

actcacctga caatcagagg gcgaggtatt cagctcaacg acgagtcggt gagctcctcg  27120 

cttggtctcc gtccggacgg gacatttcag atcggcggcg ccggccgctc ttcattcacg  27180 

cctcgtcagg caatcctaac tctgcagacc tcgtcctctg agccgcgctc tggaggcatt  27240 

ggaactctgc aatttattga ggagtttgtg ccatcggtct actttaaccc cttctcggga  27300 

cctcccggcc actatccgga tcaatttatt cctaactttg acgcggtaaa ggactcggcg  27360 

gacggctacg actgaatgtt aagtggagag gcagagcaac tgcgcctgaa acacctggtc  27420 

cactgtcgcc gccacaagtg ctttgcccgc gactccggtg agttttgcta ctttgaattg  27480 

cccgaggatc atatcgaggg cccggcgcac ggcgtccggc ttaccgccca gggagagctt  27540 

gcccgtagcc tgattcggga gtttacccag cgccccctgc tagttgagcg ggacagggga  27600 

ccctgtgttc tcactgtgat ttgcaactgt cctaaccctg gattacatca agatctttgt  27660 

tgccatctct gtgctgagta taataaatac agaaattaaa atatactggg gctcctatcg  27720 

ccatcctgta aacgccaccg tcttcacccg cccaagcaaa ccaaggcgaa ccttacctgg  27780 

tacttttaac atctctccct ctgtgattta caacagtttc aacccagacg gagtgagtct  27840 

acgagagaac ctctccgagc tcagctactc catcagaaaa aacaccaccc tccttacctg  27900 

ccgggaacgt acgagtgcgt caccggccgc tgcaccacac ctaccgcctg accgtaaacc  27960 

agactttttc cggacagacc tcaataactc tgtttaccag aacaggaggt gagcttagaa  28020 

aacccttagg gtattaggcc aaaggcgcag ctactgtggg gtttatgaac aattcaagca  28080 

actctacggg ctattctaat tcaggtttct ctagaatcgg ggttggggtt attctctgtc  28140 

ttgtgattct ctttattctt atactaacgc ttctctgcct aaggctcgcc gcctgctgtg  28200 

tgcacatttg catttattgt cagcttttta aacgctgggg tcgccaccca agatgattag  28260 

gtacataatc ctaggtttac tcacccttgc gtcagcccac ggtaccaccc aaaaggtgga  28320 

ttttaaggag ccagcctgta atgttacatt cgcagctgaa gctaatgagt gcaccactct  28380 

tataaaatgc accacagaac atgaaaagct gcttattcgc cacaaaaaca aaattggcaa  28440 

gtatgctgtt tatgctattt ggcagccagg tgacactaca gagtataatg ttacagtttt  28500 

ccagggtaaa agtcataaaa cttttatgta tacttttcca ttttatgaaa tgtgcgacat  28560 

taccatgtac atgagcaaac agtataagtt gtggccccca caaaattgtg tggaaaacac  28620 

tggcactttc tgctgcactg ctatgctaat tacagtgctc gctttggtct gtaccctact  28680 

ctatattaaa tacaaaagca gacgcagctt tattgaggaa aagaaaatgc cttaatttac  28740 

taagttacaa agctaatgtc accactaact gctttactcg ctgcttgcaa aacaaattca  28800 

aaaagttagc attataatta gaataggatt taaacccccc ggtcatttcc tgctcaatac  28860 

cattcccctg aacaattgac tctatgtggg atatgctcca gcgctacaac cttgaagtca  28920 

ggcttcctgg atgtcagcat ctgactttgg ccagcacctg tcccgcggat ttgttccagt  28980 

ccaactacag cgacccaccc taacagagat gaccaacaca accaacgcgg ccgccgctac  29040 

cggacttaca tctaccacaa atacacccca agtttctgcc tttgtcaata actgggataa  29100 

cttgggcatg tggtggttct ccatagcgct tatgtttgta tgccttatta ttatgtggct  29160 

catctgctgc ctaaagcgca aacgcgcccg accacccatc tatagtccca tcattgtgct  29220 

acacccaaac aatgatggaa tccatagatt ggacggactg aaacacatgt tcttttctct  29280 

tacagtatga ttaaatgaga catgattcct cgagttttta tattactgac ccttgttgcg  29340 

cttttttgtg cgtgctccac attggctgcg gtttctcaca tcgaagtaga ctgcattcca  29400 

gccttcacag tctatttgct ttacggattt gtcaccctca cgctcatctg cagcctcatc  29460 

actgtggtca tcgcctttat ccagtgcatt gactgggtct gtgtgcgctt tgcatatctc  29520 

agacaccatc cccagtacag ggacaggact atagctgagc ttcttagaat tctttaatta  29580 

tgaaatttac tgtgactttt ctgctgatta tttgcaccct atctgcgttt tgttccccga  29640 

cctccaagcc tcaaagacat atatcatgca gattcactcg tatatggaat attccaagtt  29700 

gctacaatga aaaaagcgat ctttccgaag cctggttata tgcaatcatc tctgttatgg  29760 

tgttctgcag taccatctta gccctagcta tatatcccta ccttgacatt ggctggaacg  29820 

caatagatgc catgaaccac ccaactttcc ccgcgcccgc tatgcttcca ctgcaacaag  29880 

ttgttgccgg cggctttgtc ccagccaatc agcctcgccc accttctccc acccccactg  29940 

aaatcagcta ctttaatcta acaggaggag atgactgaca ccctagatct agaaatggac  30000 

ggaattatta cagagcagcg cctgctagaa agacgcaggg cagcggccga gcaacagcgc  30060 

atgaatcaag agctccaaga catggttaac ttgcaccagt gcaaaagggg tatcttttgt  30120 

ctcgtaaagc aggccaaagt cacctacgac agtaatacca ccggacaccg ccttagctac  30180 

aagttgccaa ccaagcgtca gaaattggtg gtcatggtgg gagaaaagcc cattaccata  30240 

actcagcact cggtagaaac cgaaggctgc attcactcac cttgtcaagg acctgaggat  30300 

ctctgcaccc ttattaagac cctgtgcggt ctcaaagatc ttattccctt taactaataa  30360 

aaaaaaataa taaagcatca cttacttaaa atcagttagc aaatttctgt ccagtttatt  30420 

cagcagcacc tccttgccct cctcccagct ctggtattgc agcttcctcc tggctgcaaa  30480 

ctttctccac aatctaaatg gaatgtcagt ttcctcctgt tcctgtccat ccgcacccac  30540 

tatcttcatg ttgttgcaga tgaagcgcgc aagaccgtct gaagatacct tcaaccccgt  30600 

gtatccatat gacacggaaa ccggtcctcc aactgtgcct tttcttactc ctccctttgt  30660 

atcccccaat gggtttcaag agagtccccc tggggtactc tctttgcgcc tatccgaacc  30720 

tctagttacc tccaatggca tgcttgcgct caaaatgggc aacggcctct ctctggacga  30780 

ggccggcaac cttacctccc aaaatgtaac cactgtgagc ccacctctca aaaaaaccaa  30840 

gtcaaacata aacctggaaa tatctgcacc cctcacagtt acctcagaag ccctaactgt  30900 

ggctgccgcc gcacctctaa tggtcgcggg caacacactc accatgcaat cacaggcccc  30960 

gctaaccgtg cacgactcca aacttagcat tgccacccaa ggacccctca cagtgtcaga  31020 

aggaaagcta gccctgcaaa catcaggccc cctcaccacc accgatagca gtacccttac  31080 

tatcactgcc tcaccccctt taactactgc cactggtagc ttgggcattg acttgaaaga  31140 

gcccatttat acacaaaatg gaaaactagg actaaagtac ggggctcctt tgcatgtaac  31200 

agacgaccta aacactttga ccgtagcaac tggtccaggt gtgactatta ataatacttc  31260 

cttgcaaact aaagttactg gagccttggg ttttgattca caaggcaata tgcaacttaa  31320 

tgtagcagga ggactaagga ttgattctca aaacagacgc cttatacttg atgttagtta  31380 

tccgtttgat gctcaaaacc aactaaatct aagactagga cagggccctc tttttataaa  31440 

ctcagcccac aacttggata ttaactacaa caaaggcctt tacttgttta cagcttcaaa  31500 

caattccaaa aagcttgagg ttaacctaag cactgccaag gggttgatgt ttgacgctac  31560 

agccatagcc attaatgcag gagatgggct tgaatttggt tcacctaatg caccaaacac  31620 

aaatcccctc aaaacaaaaa ttggccatgg cctagaattt gattcaaaca aggctatggt  31680 

tcctaaacta ggaactggcc ttagttttga cagcacaggt gccattacag taggaaacaa  31740 

aaataatgat aagctaactt tgtggaccac accagctcca tctcctaact gtagactaaa  31800 

tgcagagaaa gatgctaaac tcactttggt cttaacaaaa tgtggcagtc aaatacttgc  31860 

tacagtttca gttttggctg ttaaaggcag tttggctcca atatctggaa cagttcaaag  31920 

tgctcatctt attataagat ttgacgaaaa tggagtgcta ctaaacaatt ccttcctgga  31980 

cccagaatat tggaacttta gaaatggaga tcttactgaa ggcacagcct atacaaacgc  32040 

tgttggattt atgcctaacc tatcagctta tccaaaatct cacggtaaaa ctgccaaaag  32100 

taacattgtc agtcaagttt acttaaacgg agacaaaact aaacctgtaa cactaaccat  32160 

tacactaaac ggtacacagg aaacaggaga cacaactcca agtgcatact ctatgtcatt  32220 

ttcatgggac tggtctggcc acaactacat taatgaaata tttgccacat cctcttacac  32280 

tttttcatac attgcccaag aataaagaat cgtttgtgtt atgtttcaac gtgtttattt  32340 

ttcaattgca gaaaatttca agtcattttt cattcagtag tatagcccca ccaccacata  32400 

gcttatacag atcaccgtac cttaatcaaa ctcacagaac cctagtattc aacctgccac  32460 

ctccctccca acacacagag tacacagtcc tttctccccg gctggcctta aaaagcatca  32520 

tatcatgggt aacagacata ttcttaggtg ttatattcca cacggtttcc tgtcgagcca  32580 

aacgctcatc agtgatatta ataaactccc cgggcagctc acttaagttc atgtcgctgt  32640 

ccagctgctg agccacaggc tgctgtccaa cttgcggttg cttaacgggc ggcgaaggag  32700 

aagtccacgc ctacatgggg gtagagtcat aatcgtgcat caggataggg cggtggtgct  32760 

gcagcagcgc gcgaataaac tgctgccgcc gccgctccgt cctgcaggaa tacaacatgg  32820 

cagtggtctc ctcagcgatg attcgcaccg cccgcagcat aaggcgcctt gtcctccggg  32880 

cacagcagcg caccctgatc tcacttaaat cagcacagta actgcagcac agcaccacaa  32940 

tattgttcaa aatcccacag tgcaaggcgc tgtatccaaa gctcatggcg gggaccacag  33000 

aacccacgtg gccatcatac cacaagcgca ggtagattaa gtggcgaccc ctcataaaca  33060 

cgctggacat aaacattacc tcttttggca tgttgtaatt caccacctcc cggtaccata  33120 

taaacctctg attaaacatg gcgccatcca ccaccatcct aaaccagctg gccaaaacct  33180 

gcccgccggc tatacactgc agggaaccgg gactggaaca atgacagtgg agagcccagg  33240 

actcgtaacc atggatcatc atgctcgtca tgatatcaat gttggcacaa cacaggcaca  33300 

cgtgcataca cttcctcagg attacaagct cctcccgcgt tagaaccata tcccagggaa  33360 

caacccattc ctgaatcagc gtaaatccca cactgcaggg aagacctcgc acgtaactca  33420 

cgttgtgcat tgtcaaagtg ttacattcgg gcagcagcgg atgatcctcc agtatggtag  33480 

cgcgggtttc tgtctcaaaa ggaggtagac gatccctact gtacggagtg cgccgagaca  33540 

accgagatcg tgttggtcgt agtgtcatgc caaatggaac gccggacgta gtcatatttc  33600 

ctgaagcaaa accaggtgcg ggcgtgacaa acagatctgc gtctccggtc tcgccgctta  33660 

gatcgctctg tgtagtagtt gtagtatatc cactctctca aagcatccag gcgccccctg  33720 

gcttcgggtt ctatgtaaac tccttcatgc gccgctgccc tgataacatc caccaccgca  33780 

gaataagcca cacccagcca acctacacat tcgttctgcg agtcacacac gggaggagcg  33840 

ggaagagctg gaagaaccat gttttttttt ttattccaaa agattatcca aaacctcaaa  33900 

atgaagatct attaagtgaa cgcgctcccc tccggtggcg tggtcaaact ctacagccaa  33960 

agaacagata atggcatttg taagatgttg cacaatggct tccaaaaggc aaacggccct  34020 

cacgtccaag tggacgtaaa ggctaaaccc ttcagggtga atctcctcta taaacattcc  34080 

agcaccttca accatgccca aataattctc atctcgccac cttctcaata tatctctaag  34140 

caaatcccga atattaagtc cggccattgt aaaaatctgc tccagagcgc cctccacctt  34200 

cagcctcaag cagcgaatca tgattgcaaa aattcaggtt cctcacagac ctgtataaga  34260 

ttcaaaagcg gaacattaac aaaaataccg cgatcccgta ggtcccttcg cagggccagc  34320 

tgaacataat cgtgcaggtc tgcacggacc agcgcggcca cttccccgcc aggaaccatg  34380 

acaaaagaac ccacactgat tatgacacgc atactcggag ctatgctaac cagcgtagcc  34440 

ccgatgtaag cttgttgcat gggcggcgat ataaaatgca aggtgctgct caaaaaatca  34500 

ggcaaagcct cgcgcaaaaa agaaagcaca tcgtagtcat gctcatgcag ataaaggcag  34560 

gtaagctccg gaaccaccac agaaaaagac accatttttc tctcaaacat gtctgcgggt  34620 

ttctgcataa acacaaaata aaataacaaa aaaacattta aacattagaa gcctgtctta  34680 

caacaggaaa aacaaccctt ataagcataa gacggactac ggccatgccg gcgtgaccgt  34740 

aaaaaaactg gtcaccgtga ttaaaaagca ccaccgacag ctcctcggtc atgtccggag  34800 

tcataatgta agactcggta aacacatcag gttgattcac atcggtcagt gctaaaaagc  34860 

gaccgaaata gcccggggga atacataccc gcaggcgtag agacaacatt acagccccca  34920 

taggaggtat aacaaaatta ataggagaga aaaacacata aacacctgaa aaaccctcct  34980 

gcctaggcaa aatagcaccc tcccgctcca gaacaacata cagcgcttcc acagcggcag  35040 

ccataacagt cagccttacc agtaaaaaag aaaacctatt aaaaaaacac cactcgacac  35100 

ggcaccagct caatcagtca cagtgtaaaa aagggccaag tgcagagcga gtatatatag  35160 

gactaaaaaa tgacgtaacg gttaaagtcc acaaaaaaca cccagaaaac cgcacgcgaa  35220 

cctacgccca gaaacgaaag ccaaaaaacc cacaacttcc tcaaatcgtc acttccgttt  35280 

tcccacgtta cgtcacttcc cattttaaga aaactacaat tcccaacaca tacaagttac  35340 

tccgccctaa aacctacgtc acccgccccg ttcccacgcc ccgcgccacg tcacaaactc  35400 

caccccctca ttatcatatt ggcttcaatc caaaataagg tatattattg atgatgttaa  35460 

ttaagaattc ggatctgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc  35520 

ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga  35580 

ccatcaggga cagcttcaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct  35640 

ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca  35700 

gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct  35760 

cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc  35820 

gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt  35880 

tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc  35940 

cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc  36000 

cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg  36060 

gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc  36120 

agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag  36180 

cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga  36240 

tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat  36300 

tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatc aatctaaagt  36360 

atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca  36420 

gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg  36480 

atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca  36540 

ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt  36600 

cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt  36660 

agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca  36720 

cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca  36780 

tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga  36840 

agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact  36900 

gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga  36960 

gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg  37020 

ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc  37080 

tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga  37140 

tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat  37200 

gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt  37260 

caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt  37320 

atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac  37380 

gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc  37440 

tttcgtcttc aagaattgga tccgaattct taat                              37474 

 
           
             8  
             37474  
             DNA  
             Artificial Sequence  
             
               pMRKAd5HIV-1 gag vector, non-coding  
             
           
            8 

aagaattaat tgtagtagtt attatatgga ataaaaccta acttcggtta tactattact     60 

cccccacctc aaacactgca ccgcgccccg cacccttgcc ccgcccactg catcatcaca    120 

ccgccttcac actacaacgt tcacaccgcc ttgtgtacat tcgctgccta caccgttttc    180 

actgcaaaaa ccacacgcgg ccacatgtgt ccttcactgt taaaagcgcg ccaaaatccg    240 

cctacaacat catttaaacc cgcattggct cattctaaac cggtaaaagc gcccttttga    300 

cttattctcc ttcactttag acttattaaa acacaatgag tatcgcgcat tataaacaga    360 

tcccggcgcc cctgaaactg gcaaatgcac ctctgagcgg gtccacaaaa agagtccaca    420 

aaaggcgcaa ggcccagttt caaccgcaaa ataataatat ccgccggcgc taggtaacgt    480 

atgcaacata ggtatagtat tatacatgta aatataaccg agtacaggtt gtaatggcgg    540 

tacaactgta actaataact gatcaataat tatcattagt taatgcccca gtaatcaagt    600 

atcgggtata tacctcaagg cgcaatgtat tgaatgccat ttaccgggcg gaccgactgg    660 

cgggttgctg ggggcgggta actgcagtta ttactgcata caagggtatc attgcggtta    720 

tccctgaaag gtaactgcag ttacccacct cataaatgcc atttgacggg tgaaccgtca    780 

tgtagttcac atagtatacg gttcatgcgg gggataactg cagttactgc catttaccgg    840 

gcggaccgta atacgggtca tgtactggaa taccctgaaa ggatgaaccg tcatgtagat    900 

gcataatcag tagcgataat ggtaccacta cgccaaaacc gtcatgtagt tacccgcacc    960 

tatcgccaaa ctgagtgccc ctaaaggttc agaggtgggg taactgcagt taccctcaaa   1020 

caaaaccgtg gttttagttg ccctgaaagg ttttacagca ttgttgaggc ggggtaactg   1080 

cgtttacccg ccatccgcac atgccaccct ccagatatat tcgtctcgag caaatcactt   1140 

ggcagtctag cggacctctg cggtaggtgc gacaaaactg gaggtatctt ctgtggccct   1200 

ggctaggtcg gaggcgccgg cccttgccac gtaaccttgc gcctaagggg cacggttctc   1260 

actctagatg gtacccacga tcccgaagac acgacagacc accactcgac ctgttcaccc   1320 

tcttctagtc cgactccgga ccaccgttct tcttcatgtt cgatttcgtg taacacaccc   1380 

ggaggtccct cgacctctcc aaacgacact tgggaccgga cgacctctgg agactcccca   1440 

cgtccgtcta ggacccggtc gaggtcggga gggacgtttg tccgagactc ctcgactcca   1500 

gggacatgtt gtgtcaccga tgggacatga cacacgtggt cttctaacta cacttcctgt   1560 

ggttcctccg ggacctcttc taactcctcc tcgtcttgtt caggttcttc ttccgggtcg   1620 

tccgacgacg accgtgtccg ttgaggtcgg tccacagggt cttgatgggg taacacgtct   1680 

tggaggtccc ggtctaccac gtggtccggt agaggggggc ctgggactta cggacccact   1740 

tccaccacct cctcttccgg aagaggggac tccactaggg gtacaagaga cgggacagac   1800 

tcccacggtg gggggtcctg gacttgtggt acgacttgtg tcaccccccg gtagtccgac   1860 

ggtacgtcta cgacttcctc tggtagttac tcctccgacg actcaccctg tccgacgtag   1920 

gacacgtgcg accggggtaa cgggggccgg tctactccct cgggtccccg agactgtaac   1980 

gaccgtggtg gaggtgggag gtcctcgtct aaccgaccta ctggttgttg ggggggtagg   2040 

gacaccccct ttagatgttc tccacctagt aggacccgga cttgttctaa cactcctaca   2100 

tgagggggtg gaggtaggac ctgtagtccg tcccggggtt cctcgggaag tccctgatac   2160 

acctgtccaa gatgttctgg gactcccgac tcgtccggag ggtcctccac ttcttgacct   2220 

actgtctctg ggacgaccac gtcttacggt tgggactgac gttctggtag gacttccggg   2280 

acccgggacg acggtgggac ctcctctact actgtcggac ggtcccccac cccccgggac   2340 

cagtgttccg gtcccacgac cgactccggt acagggtcca ctggttgagg cggtggtagt   2400 

actacgtctc cccgttgaag tccttggtct ccttctgtca cttcacgaag ttgacaccgt   2460 

tccacccggt gtaacggttc ttgacatccc gggggtcctt cttcccgacg accttcacac   2520 

cgttcctccc ggtggtctac ttcctgacgt tactctccgt ccggttgaag gacccgtttt   2580 

agaccgggag ggtgttcccg tccggaccgt tgaaggaggt caggtccgga ctcgggtgtc   2640 

ggggagggct cctcaggaag tccaaacccc tcctcttctg gtgggggtcg gtcttcgtcc   2700 

tcgggtaact gttcctcgac atgggggacc ggagggactc cagggacaaa ccgttgctgg   2760 

ggaggagggt cattttattt cgggcccgtc tagacgacac ggaagatcaa cggtcggtag   2820 

acaacaaacg gggagggggc acggaaggaa ctgggacctt ccacggtgag ggtgacagga   2880 

aaggattatt ttactccttt aacgtagcgt aacagactca tccacagtaa gataagaccc   2940 

cccaccccac cccgtcctgt cgttccccct cctaaccctt ctgttatcgt ccgtacgacc   3000 

cctacgccac ccgagatacc ggctagccgc gcggcatgac tttacacacc cgcaccgaat   3060 

tcccaccctt tcttatatat tccaccccca gaatacatca aaacatagac aaaacgtcgt   3120 

cggcggcggc ggtactcgtg gttgagcaaa ctaccttcgt aacactcgag tataaactgt   3180 

tgcgcgtacg ggggtacccg gccccacgca gtcttacact acccgaggtc gtaactacca   3240 

gcggggcagg acgggcgttt gagatgatgg aactggatgc tctggcacag accttgcggc   3300 

aacctctgac gtcggaggcg gcggcgaagt cggcgacgtc ggtggcgggc gccctaacac   3360 

tgactgaaac gaaaggactc gggcgaacgt ttgtcacgtc gaagggcaag taggcgggcg   3420 

ctactgttca actgccgaga aaaccgtgtt aacctaagaa actgggccct tgaattacag   3480 

caaagagtcg tcgacaacct agacgcggtc gtccaaagac gggacttccg aaggagggga   3540 

gggttacgcc aaattttgta tttatttttt ggtctgagac aaacctaaac ctagttcgtt   3600 

cacagaacga cagaaataaa tccccaaaac gcgcgcgcca tccgggccct ggtcgccaga   3660 

gccagcaact cccaggacac ataaaaaagg tcctgcacca tttccactga gacctacaag   3720 

tctatgtacc cgtattcggg cagagacccc acctccatcg tggtgacgtc tcgaagtacg   3780 

acgccccacc acaacatcta ctaggtcagc atcgtcctcg cgacccgcac cacggatttt   3840 

tacagaaagt catcgttcga ctaacggtcc ccgtccggga accacattca caaatgtttc   3900 

gccaattcga ccctacccac gtatgcaccc ctatactcta cgtagaacct gacataaaaa   3960 

tccaaccgat acaagggtcg gtatagggag gcccctaagt acaacacgtc ttggtggtcg   4020 

tgtcacatag gccacgtgaa ccctttaaac agtacatcga atcttccttt acgcaccttc   4080 

ttgaacctct gcgggaacac tggaggttct aaaaggtacg taagcaggta ttactaccgt   4140 

tacccgggtg cccgccgccg gacccgcttc tataaagacc ctagtgattg cagtatcaac   4200 

acaaggtcct actctagcag tatccggtaa aaatgtttcg cgcccgcctc ccacggtctg   4260 

acgccatatt accaaggtag gccgggtccc cgcatcaatg ggagtgtcta aacgtaaagg   4320 

gtgcgaaact caagtctacc cccctagtac agatggacgc cccgctactt cttttgccaa   4380 

aggccccatc ccctctagtc gacccttctt tcgtccaagg actcgtcgac gctgaatggc   4440 

gtcggccacc cgggcattta gtgtggataa tggccgacgt tgaccatcaa ttctctcgac   4500 

gtcgacggca gtagggactc gtccccccgg tgaagcaatt cgtacaggga ctgagcgtac   4560 

aaaagggact ggtttaggcg gtcttccgcg agcggcgggt cgctatcgtc aagaacgttc   4620 

cttcgtttca aaaagttgcc aaactctggc aggcggcatc cgtacgaaaa ctcgcaaact   4680 

ggttcgtcaa ggtccgccag ggtgtcgagc cagtggacga gatgccgtag agctaggtcg   4740 

tatagaggag caaagcgccc aaccccgccg aaagcgacat gccgtcatca gccacgagca   4800 

ggtctgcccg gtcccagtac agaaaggtgc ccgcgtccca ggagcagtcg catcagaccc   4860 

agtgccactt ccccacgcga ggcccgacgc gcgaccggtc ccacgcgaac tccgaccagg   4920 

acgaccacga cttcgcgacg gccagaagcg ggacgcgcag ccggtccatc gtaaactggt   4980 

accacagtat caggtcgggg aggcgccgca ccgggaaccg cgcgtcgaac gggaacctcc   5040 

tccgcggcgt gctccccgtc acgtctgaaa actcccgcat ctcgaacccg cgctctttat   5100 

ggctaaggcc cctcatccgt aggcgcggcg tccggggcgt ctgccagagc gtaaggtgct   5160 

cggtccactc gagaccggca agccccagtt tttggtccaa agggggtacg aaaaactacg   5220 

caaagaatgg agaccaaagg tactcggcca caggtgcgag ccactgcttt tccgacaggc   5280 

acaggggcat atgtctgaac tctccggaca ggagctcgcc acaaggcgcc aggaggagca   5340 

tatctttgag cctggtgaga ctctgtttcc gagcgcaggt ccggtcgtgc ttcctccgat   5400 

tcaccctccc catcgccagc aacaggtgat cccccaggtg agcgaggtcc cacacttctg   5460 

tgtacagcgg gagaagccgt agttccttcc actaaccaaa catccacatc cggtgcactg   5520 

gcccacaagg acttcccccc gatattttcc cccacccccg cgcaagcagg agtgagagaa   5580 

ggcgtagcga cagacgctcc cggtcgacaa ccccactcat gagggagact tttcgcccgt   5640 

actgaagacg cgattctaac agtcaaaggt ttttgctcct cctaaactat aagtggaccg   5700 

ggcgccacta cggaaactcc caccggcgta ggtagaccag tcttttctgt tagaaaaaca   5760 

acagttcgaa ccaccgtttg ctgggcatct cccgcaacct gtcgttgaac cgctacctcg   5820 

cgtcccaaac caaaaacagc gctagccgcg cgaggaaccg gcgctacaaa tcgacgtgca   5880 

taagcgcgcg ttgcgtggcg gtaagccctt tctgccacca cgcgagcagc ccgtggtcca   5940 

cgtgcgcggt tggcgccaac acgtcccact gttccagttg cgaccaccga tggagaggcg   6000 

catccgcgag caaccaggtc gtctccgccg gcgggaacgc gctcgtctta ccgccatccc   6060 

ccagatcgac gcagagcagg ccccccagac gcaggtgcca tttctggggc ccgtcgtccg   6120 

cgcgcagctt catcagatag aacgtaggaa cgttcagatc gcggacgacg gtacgcgccc   6180 

gccgttcgcg cgcgagcata cccaactcac cccctggggt accgtacccc acccactcgc   6240 

gcctccgcat gtacggcgtt tacagcattt gcatctcccc gagagactca taaggttcta   6300 

tacatcccat cgtagaaggt ggcgcctacg accgcgcgtg cattagcata tcaagcacgc   6360 

tccctcgctc ctccagccct ggctccaacg atgcccgccc gacgagacga gccttctgat   6420 

agacggactt ctaccgtaca ctcaacctac tataccaacc tgcgaccttc tgcaacttcg   6480 

accgcagaca ctctggatgg cgcagtgcgt gcttcctccg catcctcagc gcgtcgaaca   6540 

actggtcgag ccgccactgg acgtgcagat cccgcgtcat caggtcccaa aggaactact   6600 

acagtatgaa taggacaggg aaaaaaaagg tgtcgagcgc caactcctgt ttgagaagcg   6660 

ccagaaaggt catgagaacc tagcctttgg gcagccggag gcttgccatt ctcggatcgt   6720 

acatcttgac caactgccgg accatccgcg tcgtagggaa aagatgccca tcgcgcatac   6780 

ggacgcgccg gaaggcctcg ctccacaccc actcgcgttt ccacagggac tggtactgaa   6840 

actccatgac cataaacttc agtcacagca gcgtaggcgg gacgagggtc tcgtttttca   6900 

ggcacgcgaa aaaccttgcg cctaaaccgt cccgcttcca ctgtagcaac ttctcataga   6960 

aagggcgcgc tccgtatttc aacgcacact acgccttccc agggccgtgg agccttgcca   7020 

acaattaatg gacccgccgc tcgtgctaga gcagtttcgg caactacaac accgggtgtt   7080 

acatttcaag gttcttcgcg ccctacggga actaccttcc gttaaaaaat tcaaggagca   7140 

tccactcgag aagtcccctc gactcgggca cgagactttc ccgggtcaga cgttctactc   7200 

ccaaccttcg ctgcttactc gaggtgtcca gtgcccggta atcgtaaacg tccaccagcg   7260 

ctttccagga tttgaccgct ggataccggt aaaaaagacc ccactacgtc atcttccatt   7320 

cgcccagaac aagggtcgcc agggtaggtt ccaagcgccg atccagagcg cgccgtcagt   7380 

gatctccgag tagaggcggc ttgaagtact ggtcgtactt cccgtgctcg acgaagggtt   7440 

tccgggggta ggttcatatc cagagatgta gcatccactg tttctctgcg agccacgctc   7500 

ctacgctcgg ctagcccttc ttgacctaga gggcggtggt taacctcctc accgataact   7560 

acaccacttt catcttcagg gacgctgccc ggcttgtgag cacgaccgaa aacatttttg   7620 

cacgcgtcat gaccgtcgcc acgtgcccga catgtaggac gtgctccaac tggactgctg   7680 

gcgcgtgttc cttcgtctca cccttaaact cggggagcgg accgcccaaa ccgaccacca   7740 

gaagatgaag ccgacgaaca ggaactggca gaccgacgag ctcccctcaa tgccacctag   7800 

cctggtggtg cggcgcgctc gggtttcagg tctacaggcg cgcgccgcca gcctcgaact   7860 

actgttgtag cgcgtctacc ctcgacaggt accagacctc gagggcgccg cagtccagtc   7920 

cgccctcgag gacgtccaaa tggagcgtat ctgcccagtc ccgcgcccga tctaggtcca   7980 

ctatggatta aaggtccccg accaaccacc gccgcagcta ccgaacgttc tccggcgtag   8040 

gggcgccgcg ctgatgccat ggcgcgccgc ccgccacccg gcgcccccac aggaacctac   8100 

tacgtagatt ttcgccactg cgcccgctcg ggggcctcca tcccccccga ggcctgggcg   8160 

gccctctccc ccgtccccgt gcagccgcgg cgcgcgcccg tcctcgacca cgacgcgcgc   8220 

atccaacgac cgcttgcgct gctgcgccgc caactagagg acttagaccg cggagacgca   8280 

cttctgctgc ccgggccact cgaacttgga ctttctctca agctgtctta gttaaagcca   8340 

cagcaactgc cgccggaccg cgttttagag gacgtgcaga ggactcaaca gaactatccg   8400 

ctagagccgg tacttgacga gctagagaag gaggacctct agaggcgcag gccgagcgag   8460 

gtgccaccgc cgctccagca acctttacgc ccggtactcg acgctcttcc gcaactccgg   8520 

agggagcaag gtctgcgccg acatctggtg cgggggaagc cgtagcgccc gcgcgtactg   8580 

gtggacgcgc tctaactcga ggtgcacggc ccgcttctgc cgcatcaaag cgtccgcgac   8640 

tttctccatc aactcccacc accgccacac aagacggtgc ttcttcatgt attgggtcgc   8700 

agcgttgcac ctaagcaact atagggggtt ccggagttcc gcgaggtacc ggagcatctt   8760 

caggtgccgc ttcaactttt tgaccctcaa cgcgcggctg tgccaattga ggaggaggtc   8820 

ttctgcctac tcgagccgct gtcacagcgc gtggagcgcg agtttccgat gtccccggag   8880 

aagaagaaga agttagagga gaaggtattc ccggagggga agaagaagaa gaccgccgcc   8940 

accccctccc ccctgtgccg ccgctgctgc cgcgtggccc tccgccagct gtttcgcgag   9000 

ctagtagagg ggcgccgctg ccgcgtacca gagccactgc cgcgccggca agagcgcccc   9060 

cgcgtcaacc ttctgcggcg ggcagtacag ggccaatacc caaccgcccc ccgacggtac   9120 

gccgtcccta tgccgcgatt gctacgtaga gttgttaaca acacatccat gaggcggcgg   9180 

ctccctggac tcgctcaggc gtagctggcc tagccttttg gagagctctt tccgcagatt   9240 

ggtcagtgtc agcgttccat ccgactcgtg gcaccgcccg ccgtcgcccg ccgccagccc   9300 

caacaaagac cgcctccacg acgactacta cattaatttc atccgccaga actctgccgc   9360 

ctaccagctg tcttcgtggt acaggaaccc aggccggacg acttacgcgt ccgccagccg   9420 

gtacggggtc cgaagcaaaa ctgtagccgc gtccagaaac atcatcagaa cgtactcgga   9480 

aagatggccg tgaagaagaa gaggaaggag aacaggacgt agagaacgta gatagcgacg   9540 

ccgccgccgc ctcaaaccgg catccaccgc gggagaagga gggtacgcac actggggctt   9600 

cggggagtag ccgacttcgt cccgatccag ccgctgttgc gcgagccgat tataccggac   9660 

gacgtggacg cactcccatc tgaccttcag taggtacagg tgtttcgcca ccatacgcgg   9720 

gcacaactac cacattcacg tcaaccggta ttgcctggtc aattgccaga ccactgggcc   9780 

gacgctctcg agccacatgg actctgcgct cattcgggag ctcagtttat gcatcagcaa   9840 

cgttcaggcg tggtccatga ccatagggtg gtttttcacg ccgccgccga ccgccatctc   9900 

cccggtcgca tcccaccggc cccgaggccc ccgctctaga aggttgtatt ccgctactat   9960 

aggcatctac atggacctgt aggtccacta cggccgccgc caccacctcc gcgcgccttt  10020 

cagcgcctgc gccaaggtct acaacgcgtc gccgtttttc acgaggtacc agccctgcga  10080 

gaccggccag tccgcgcgcg ttagcaactg cgagatctgg cacgttttcc tctcggacat  10140 

tcgcccgtga gaaggcacca gaccacctat ttaagcgttc ccatagtacc gcctgctggc  10200 

cccaagctcg gggcataggc cggcaggcgg cactaggtac gccaatggcg ggcgcacagc  10260 

ttgggtccac acgctgcagt ctgttgcccc ctcacgagga aaaccgaagg aaggtccgcg  10320 

ccgccgacga cgcgatcgaa aaaaccggtg accggcgcgc gtcgcattcg ccaatccgac  10380 

ctttcgcttt cgtaattcac cgagcgaggg acatcggcct cccaataaaa ggttcccaac  10440 

tcagcgccct gggggccaag ctcagagcct ggccggcctg acgccgcttg cccccaaacg  10500 

gaggggcagt acgttctggg gcgaacgttt aaggaggcct ttgtccctgc tcggggaaaa  10560 

aacgaaaagg gtctacgtag gccacgacgc cgtctacgcg gggggaggag tcgtcgccgt  10620 

tctcgttctc gtcgccgtct gtacgtcccg tgggagggga ggaggatggc gcagtcctcc  10680 

ccgctgtagg cgccaactgc gccgtcgtct accactaatg cttgggggcg ccgcggcccg  10740 

ggccgtgatg gacctgaacc tcctcccgct cccggaccgc gccgatcctc gcgggagagg  10800 

actcgccgtg ggttcccacg tcgacttcgc actatgcgca ctccgcatgc acggcgccgt  10860 

cttggacaaa gcgctggcgc tccctctcct cgggctcctc tacgccctag ctttcaaggt  10920 

gcgtcccgcg ctcgacgccg taccggactt agcgctcgcc aacgacgcgc tcctcctgaa  10980 

actcgggctg cgcgcttggc cctaatcagg gcgcgcgcgt gtgcaccgcc ggcggctgga  11040 

ccattggcgt atgctcgtct gccacttggt cctctaattg aaagtttttt cgaaattgtt  11100 

ggtgcacgca tgcgaacacc gcgcgctcct ccaccgatat cctgactacg tagacaccct  11160 

gaaacattcg cgcgacctcg ttttgggttt atcgttcggc gagtaccgcg tcgacaagga  11220 

atatcacgtc gtgtcgtccc tgttgctccg taagtcccta cgcgacgatt tgtatcatct  11280 

cgggctcccg gcgaccgacg agctaaacta tttgtaggac gtctcgtatc accacgtcct  11340 

cgcgtcgaac tcggaccgac tgttccaccg gcggtagttg ataaggtacg aatcggaccc  11400 

gttcaaaatg cgggcgttct atatggtatg gggaatgcaa gggtatctgt tcctccattt  11460 

ctagctcccc aagatgtacg cgtaccgcga cttccacgaa tggaactcgc tgctggaccc  11520 

gcaaatagcg ttgctcgcgt aggtgttccg gcactcgcac tcggccgccg cgctcgagtc  11580 

gctggcgctc gactacgtgt cggacgtttc ccgggaccga ccgtgcccgt cgccgctatc  11640 

tctccggctc aggatgaaac tgcgcccgcg actggacgcg acccggggtt cggctgcgcg  11700 

ggacctccgt cgaccccggc ctggacccga ccgccaccgt gggcgcgcgc gaccgttgca  11760 

gccgccgcac ctccttatac tgctcctgct actcatgctc ggtctcctgc cgctcatgat  11820 

tcgccactac aaagactagt ctactacgtt ctgcgttgcc tgggccgcca cgcccgccgc  11880 

gacgtctcgg tcggcaggcc ggaattgagg tgcctgctga ccgcggtcca gtacctggcg  11940 

tagtacagcg actgacgcgc gttaggactg cgcaaggccg tcgtcggcgt ccggttggcc  12000 

gagaggcgtt aagaccttcg ccaccagggc cgcgcgcgtt tggggtgcgt gctcttccac  12060 

gaccgctagc atttgcgcga ccggcttttg tcccggtagg ccgggctgct ccggccggac  12120 

cagatgctgc gcgacgaagt cgcgcaccga gcaatgttgt cgccgttgca cgtctggttg  12180 

gacctggccg accaccccct acacgcgctc cggcaccgcg tcgcactcgc gcgcgtcgtc  12240 

gtcccgttgg acccgaggta ccaacgtgat ttgcggaagg actcatgtgt cgggcggttg  12300 

cacggcgccc ctgtcctcct gatgtggttg aaacactcgc gtgacgccga ttaccactga  12360 

ctctgtggcg tttcactcca catggtcaga cccggtctga taaaaaaggt ctggtcatct  12420 

gttccggacg tctggcattt ggactcggtc cgaaagtttt tgaacgtccc cgacaccccc  12480 

cacgcccgag ggtgtccgct ggcgcgctgg cacagatcga acgactgcgg gttgagcgcg  12540 

gacaacgacg acgattatcg cgggaagtgc ctgtcaccgt cgcacagggc cctgtgtatg  12600 

gatccagtga acgactgtga catggcgctc cggtatccag tccgcgtaca cctgctcgta  12660 

tgaaaggtcc tctaatgttc acagtcggcg cgcgaccccg tcctcctgtg cccgtcggac  12720 

ctccgttggg atttgatgga cgactggttg gccgccgtct tctaggggag caacgtgtca  12780 

aatttgtcgc tcctcctcgc gtaaaacgcg atgcacgtcg tctcgcactc ggaattggac  12840 

tacgcgctgc cccattgcgg gtcgcaccgc gacctgtact ggcgcgcgtt gtaccttggc  12900 

ccgtacatac ggagtttggc cggcaaatag ttggcggatt acctgatgaa cgtagcgcgc  12960 

cggcggcact tggggctcat aaagtggtta cggtagaact tgggcgtgac cgatggcggg  13020 

ggaccaaaga tgtggccccc taagctccac gggctcccat tgctacctaa ggagaccctg  13080 

ctgtatctgc tgtcgcacaa aaggggcgtt ggcgtctggg acgatctcaa cgttgtcgcg  13140 

ctcgtccgtc tccgccgcga cgctttcctt tcgaaggcgt ccggttcgtc gaacaggcta  13200 

gatccgcgac gccggggcgc cagtctacga tcatcgggta aaggttcgaa ctatcccaga  13260 

gaatggtcgt gagcgtggtg ggcgggcgcg gacgacccgc tcctcctcat ggatttgttg  13320 

agcgacgacg tcggcgtcgc gctttttttg gacggaggcc gtaaagggtt gttgccctat  13380 

ctctcggatc acctgttcta ctcatctacc ttctgcatgc gcgtcctcgt gtccctgcac  13440 

ggtccgggcg cgggcgggtg ggcagcagtt tccgtgctgg cagtcgcccc agaccacacc  13500 

ctcctgctac tgagccgtct gctgtcgtcg caggacctaa accctccctc accgttgggc  13560 

aaacgcgtgg aagcggggtc cgacccctct tacaaaattt tttttttttt cgtactacgt  13620 

tttatttttt gagtggttcc ggtaccgtgg ctcgcaacca aaagaacata aggggaatca  13680 

tacgccgcgc gccgctacat actccttcca ggaggaggga ggatgctctc acaccactcg  13740 

cgccgcggtc accgccgccg cgacccaaga gggaagctac gaggggacct gggcggcaaa  13800 

cacggaggcg ccatggacgc cggatggccc ccctctttgt cgtaggcaat gagactcaac  13860 

cgtggggata agctgtggtg ggcacacatg gaccacctgt tgttcagttg cctacaccgt  13920 

agggacttga tggtcttgct ggtgtcgttg aaagactggt gccagtaagt tttgttactg  13980 

atgtcgggcc ccctccgttc gtgtgtctgg tagttagaac tgctggccag cgtgaccccg  14040 

ccgctggact tttggtagga cgtatggttg tacggtttac acttgctcaa gtacaaatgg  14100 

ttattcaaat tccgcgccca ctaccacagc gcgaacggat gattcctgtt agtccacctc  14160 

gactttatgc tcacccacct caagtgcgac gggctcccgt tgatgaggct ctggtactgg  14220 

tatctggaat acttgttgcg ctagcacctc gtgatgaact ttcacccgtc tgtcttgccc  14280 

caagaccttt cgctgtagcc ccatttcaaa ctgtgggcgt tgaagtctga ccccaaactg  14340 

gggcagtgac cagaacagta cggaccccat atatgtttgc ttcggaaggt aggtctgtag  14400 

taaaacgacg gtcctacgcc ccacctgaag tgggtgtcgg cggactcgtt gaacaacccg  14460 

taggcgttcg ccgttgggaa ggtcctcccg aaatcctagt ggatgctact agacctccca  14520 

ccattgtaag ggcgtgacaa cctacacctg cggatggtcc gctcgaactt tctactgtgg  14580 

cttgtcccgc ccccaccgcg tccgccgtcg ttgtcgtcac cgtcgccgcg ccttctcttg  14640 

aggttgcgcc gtcggcgccg ttacgtcggc cacctcctgt acttgctagt acggtaagcg  14700 

ccgctgtgga aacggtgtgc ccgactcctc ttcgcgcgac tccggcttcg tcgccggctt  14760 

cgacggcggg ggcgacgcgt tgggctccag ctcttcggag tcttctttgg ccactagttt  14820 

ggggactgtc tcctgtcgtt ctttgcgtca atgttggatt attcgttact gtcgtggaag  14880 

tgggtcatgg cgtcgaccat ggaacgtatg ttgatgccgc tgggagtctg gccttaggcg  14940 

agtacctggg acgaaacgtg aggactgcat tggacgccga gcctcgtcca gatgaccagc  15000 

aacggtctgt actacgttct ggggcactgg aaggcgaggt gcgcggtcta gtcgttgaaa  15060 

ggccaccacc cgcggctcga caacgggcac gtgaggttct cgaagatgtt gctggtccgg  15120 

cagatgaggg ttgagtaggc ggtcaaatgg agagactggg tgcacaagtt agcgaaaggg  15180 

ctcttggtct aaaaccgcgc gggcggtcgg gggtggtagt ggtggcagtc acttttgcaa  15240 

ggacgagagt gtctagtgcc ctgcgatggc gacgcgttgt cgtagcctcc tcaggtcgct  15300 

cactggtaat gactgcggtc tgcggcgtgg acggggatgc aaatgttccg ggacccgtat  15360 

cagagcggcg cgcaggatag ctcggcgtga aaaactcgtt cgtacaggta ggaatatagc  15420 

gggtcgttat tgtgtccgac cccggacgcg aagggttcgt tctacaaacc gccccggttc  15480 

ttcgcgaggc tggttgtggg tcacgcgcac gcgcccgtga tggcgcgcgg gaccccgcgc  15540 

gtgtttgcgc cggcgtgacc cgcgtggtgg cagctactgc ggtagctgcg ccaccacctc  15600 

ctccgcgcgt tgatgtgcgg gtgcggcggt ggtcacaggt gtcacctgcg ccggtaagtc  15660 

tggcaccacg cgcctcgggc cgcgatacga ttttacttct ctgccgcctc cgcgcatcgt  15720 

gcagcggtgg cggcggctgg gccgtgacgg cgggttgcgc gccgccgccg ggacgaattg  15780 

gcgcgtgcag cgtggccggc tgcccgccgg tacgcccggc gagcttccga ccggcgccca  15840 

taacagtgac acggggggtc caggtccgct gctcgccggc ggcgtcgtcg gcgccggtaa  15900 

tcacgatact gagtcccagc gtccccgttg cacataaccc acgcgctgag ccaatcgccg  15960 

gacgcgcacg ggcacgcgtg ggcggggggc gcgttgatct aacgttcttt tttgatgaat  16020 

ctgagcatga caacatacat aggtcgccgc cgccgcgcgt tgcttcgata caggttcgcg  16080 

ttttagtttc ttctctacga ggtccagtag cgcggcctct agataccggg gggcttcttc  16140 

cttctcgtcc taatgttcgg ggctttcgat ttcgcccagt ttttcttttt ctttctacta  16200 

ctactacttg aactgctgct ccaccttgac gacgtgcgat ggcgcgggtc cgctgcccat  16260 

gtcacctttc cagctgcgca ttttgcacaa aacgctgggc cgtggtggca tcagaaatgc  16320 

gggccactcg cgaggtgggc gtggatgttc gcgcacatac tactccacat gccgctgctc  16380 

ctggacgaac tcgtccggtt gctcgcggag cccctcaaac ggatgccttt cgccgtattc  16440 

ctgtacgacc gcaacggcga cctgctcccg ttgggttgtg gatcggattt cgggcattgt  16500 

gacgtcgtcc acgacgggcg cgaacgtggc aggcttcttt tcgcgccgga tttcgcgctc  16560 

agaccactga accgtgggtg gcacgtcgac taccatgggt tcgcggtcgc tgaccttcta  16620 

cagaaccttt tttactggca ccttggaccc gacctcgggc tccaggcgca cgccggttag  16680 

ttcgtccacc gcggccctga cccgcacgtc tggcacctgc aagtctatgg gtgatggtca  16740 

tcgtggtcat aacggtggcg gtgtctcccg tacctctgtg tttgcagggg ccaacggagt  16800 

cgccaccgcc tacggcgcca cgtccgccag cgacgccggc gcaggttctg gagatgcctc  16860 

cacgtttgcc tgggcaccta caaagcgcaa agtcgggggg ccgcgggcgc ggcaagctcc  16920 

ttcatgccgc ggcggtcgcg cgatgacggg cttatacggg atgtaggaag gtaacgcgga  16980 

tgggggccga tagcaccgat gtggatggcg gggtcttctg ctcgttgatg ggctgcggct  17040 

tggtggtgac cttgggcggc ggcggcagcg gcagcggtcg ggcacgaccg gggctaaagg  17100 

cacgcgtccc accgagcgct tcctccgtcc tgggaccacg acggttgtcg cgcgatggtg  17160 

gggtcgtagc aaattttcgg ccagaaacac caagaacgtc tataccggga gtggacggcg  17220 

gaggcaaagg gccacggccc taaggctcct tcttacgtgg catcctcccc gtaccggccg  17280 

gtgccggact gcccgccgta cgcagcacgc gtggtggccg ccgccgcgcg cagcgtggca  17340 

gcgtacgcgc cgccatagga cggggaggaa taaggtgact agcggcgccg ctaaccgcgg  17400 

cacgggcctt aacgtaggca ccggaacgtc cgcgtctctg tgactaattt ttgttcaacg  17460 

tacacctttt tagttttatt tttcagacct gagagtgcga gcgaaccagg acattgataa  17520 

aacatcttac cttctgtagt tgaaacgcag agaccggggc gctgtgccga gcgcgggcaa  17580 

gtaccctttg accgttctat agccgtggtc gttatactcg ccaccgcgga agtcgacccc  17640 

gagcgacacc tcgccgtaat ttttaaagcc aaggtggcaa ttcttgatac cgtcgttccg  17700 

gaccttgtcg tcgtgtccgg tctacgactc cctattcaac tttctcgttt taaaggttgt  17760 

tttccaccat ctaccggacc ggagaccgta atcgccccac cacctggacc ggttggtccg  17820 

tcacgtttta ttctaattgt cattcgaact aggggcggga gggcatctcc tcggaggtgg  17880 

ccggcacctc tgtcacagag gtctccccgc accgcttttc gcaggcgcgg ggctgtccct  17940 

tctttgagac cactgcgttt atctgctcgg agggagcatg ctcctccgtg atttcgttcc  18000 

ggacgggtgg tgggcagggt agcgcgggta ccgatggcct cacgacccgg tcgtgtgtgg  18060 

gcattgcgac ctggacggag gggggcggct gtgggtcgtc tttggacacg acggtccggg  18120 

ctggcggcaa caacattggg caggatcggc gcgcagggac gcggcgcggc ggtcgccagg  18180 

cgctagcaac gccgggcatc ggtcaccgtt gaccgtttcg tgtgacttgt cgtagcaccc  18240 

agacccccac gttagggact tcgcggctgc tacgaagact atcgattgca cagcatacac  18300 

acagtacata cgcaggtaca gcggcggtct cctcgacgac tcggcggcgc gcgggcgaaa  18360 

ggttctaccg atggggaagc tactacggcg tcaccagaat gtacgtgtag agcccggtcc  18420 

tgcggagcct catggactcg gggcccgacc acgtcaaacg ggcgcggtgg ctctgcatga  18480 

agtcggactt attgttcaaa tctttggggt gccaccgcgg atgcgtgctg cactggtgtc  18540 

tggccagggt cgcaaactgc gacgccaagt agggacacct ggcactccta tgacgcatga  18600 

gcatgttccg cgccaagtgg gatcgacacc cactattggc acacgacctg taccgaaggt  18660 

gcatgaaact gtaggcgccg cacgacctgt ccccgggatg aaaattcggg atgagaccgt  18720 

gacggatgtt gcgggaccga gggttcccac ggggtttagg aacgcttacc ctacttcgac  18780 

gatgacgaga actttatttg gatcttcttc tcctgctact gttgcttctg cttcatctgc  18840 

tcgttcgact cgtcgttttt tgagtgcata aacccgtccg cggaataaga ccatatttat  18900 

aatgtttcct cccataagtt tatccacagc ttccagtttg tggatttata cggctatttt  18960 

gtaaagttgg acttggagtt tatcctctta gagtcaccat gctttgtctt taattagtac  19020 

gtcgaccctc tcaggatttt ttctgatggg gttactttgg tacaatgcca agtatacgtt  19080 

ttgggtgttt acttttacct cccgttccgt aagaacattt cgttgtttta cctttcgatc  19140 

tttcagttca cctttacgtt aaaaagagtt gatgactccg tcggcgtccg ttaccactat  19200 

tgaactgagg atttcaccat aacatgtcac ttctacatct atatctttgg ggtctgtgag  19260 

tataaagaat gtacgggtga taattccttc cattgagtgc tcttgattac ccggttgtta  19320 

gatacgggtt gtccggatta atgtaacgaa aatccctgtt aaaataacca gattacataa  19380 

tgttgtcgtg cccattatac ccacaagacc gcccggttcg tagcgtcaac ttacgacaac  19440 

atctaaacgt tctgtctttg tgtctcgaaa gtatggtcga aaacgaacta aggtaaccac  19500 

tatcttggtc catgaaaaga tacaccttag tccgacaact gtcgatacta ggtctacaat  19560 

cttaataact tttagtacct tgacttctac ttgaaggttt aatgacgaaa ggtgaccctc  19620 

cacactaatt atgtctctga gaatggttcc attttggatt ttgtccagtc cttttaccta  19680 

ccctttttct acgatgtctt aaaagtctat ttttacttta ttctcaacct ttattaaaac  19740 

ggtaccttta gttagattta cggttggaca cctctttaaa ggacatgagg ttgtatcgcg  19800 

acataaacgg gctgttcgat ttcatgtcag gaaggttgca tttttaaaga ctattgggtt  19860 

tgtggatgct gatgtacttg ttcgctcacc accgagggcc cgatcacctg acgatgtaat  19920 

tggaacctcg tgcgaccagg gaactgatat acctgttgca gttgggtaaa ttggtggtgg  19980 

cgttacgacc ggacgcgatg gcgagttaca acgacccgtt accagcgata cacgggaagg  20040 

tgtaggtcca cggagtcttc aagaaacggt aatttttgga ggaagaggac ggcccgagta  20100 

tgtggatgct caccttgaag tccttcctac aattgtacca agacgtctcg agggatcctt  20160 

tactggattc ccaactgcct cggtcgtaat tcaaactatc gtaaacggaa atgcggtgga  20220 

agaaggggta ccgggtgttg tggcggaggt gcgaactccg gtacgaatct ttgctgtggt  20280 

tgctggtcag gaaattgctg atagagaggc ggcggttgta cgagatggga tatgggcggt  20340 

tgcgatggtt gcacgggtat aggtagggga gggcgttgac ccgccgaaag gcgccgaccc  20400 

ggaagtgcgc ggaattctga ttcctttggg gtagtgaccc gagcccgatg ctgggaataa  20460 

tgtggatgag accgagatat gggatggatc taccttggaa aatggagttg gtgtggaaat  20520 

tcttccaccg gtaatggaaa ctgagaagac agtcgaccgg accgttactg gcggacgaat  20580 

gggggttgct caaactttaa ttcgcgagtc aactgcccct cccaatgttg caacgggtca  20640 

cattgtactg gtttctgacc aaggaccatg tttacgatcg attgatattg taaccgatgg  20700 

tcccgaagat atagggtctc tcgatgttcc tggcgtacat gaggaagaaa tctttgaagg  20760 

tcgggtactc ggcagtccac cacctactat gatttatgtt cctgatggtt gtccacccgt  20820 

aggatgtggt tgtgttgttg agacctaaac aaccgatgga acgggggtgg tacgcgcttc  20880 

ctgtccggat gggacgattg aaggggatag gcgaatatcc gttctggcgt caactgtcgt  20940 

aatgggtctt tttcaaagaa acgctagcgt gggaaaccgc gtagggtaag aggtcattga  21000 

aatacaggta cccgcgtgag tgtctggacc cggttttgga agagatgcgg ttgaggcggg  21060 

tgcgcgatct gtactgaaaa ctccacctag ggtacctgct cgggtgggaa gaaatacaaa  21120 

acaaacttca gaaactgcac caggcacacg tggtcggcgt ggcgccgcag tagctttggc  21180 

acatggacgc gtgcgggaag agccggccgt tgcggtgttg tatttcttcg ttcgttgtag  21240 

ttgttgtcga cggcggtacc cgaggtcact cgtccttgac tttcggtaac agtttctaga  21300 

accaacaccc ggtataaaaa acccgtggat actgttcgcg aaaggtccga aacaaagagg  21360 

tgtgttcgag cggacgcggt atcagttatg ccggccagcg ctctgacccc cgcatgtgac  21420 

ctaccggaaa cggaccttgg gcgtgagttt ttgtacgatg gagaaactcg ggaaaccgaa  21480 

aagactggtc gctgagttcg tccaaatggt caaactcatg ctcagtgagg acgcggcatc  21540 

gcggtaacga agaagggggc tggcgacata ttgcgacctt ttcaggtggg tttcgcatgt  21600 

ccccgggttg agccggcgga cacctgataa gacgacgtac aaagaggtgc ggaaacggtt  21660 

gaccggggtt tgagggtacc tagtgttggg gtggtacttg gaataatggc cccatgggtt  21720 

gaggtacgag ttgtcagggg tccatgtcgg gtgggacgca gcgttggtcc ttgtcgagat  21780 

gtcgaaggac ctcgcggtga gcgggatgaa ggcgtcggtg tcacgcgtct aatcctcgcg  21840 

gtgaagaaaa acagtgaact ttttgtacat ttttattaca tgatctctgt gaaagttatt  21900 

tccgtttacg aaaataaaca tgtgagagcc cactaataaa tgggggtggg aacggcagac  21960 

gcggcaaatt tttagtttcc ccaagacggc gcgtagcgat acgcggtgac cgtccctgtg  22020 

caacgctatg accacaaatc acgaggtgaa tttgagtccg tgttggtagg cgccgtcgag  22080 

ccacttcaaa agtgaggtgt ccgacgcgtg gtagtggttg cgcaaatcgt ccagcccgcg  22140 

gctatagaac ttcagcgtca accccggagg cgggacgcgc gcgctcaacg ctatgtgtcc  22200 

caacgtcgtg accttgtgat agtcgcggcc caccacgtgc gaccggtcgt gcgagaacag  22260 

cctctagtct aggcgcaggt ccaggaggcg caacgagtcc cgcttgcctc agttgaaacc  22320 

atcgacggaa gggtttttcc cgcgcacggg tccgaaactc aacgtgagcg tggcatcacc  22380 

gtagttttcc actggcacgg gccagacccg caatcctatg tcgcggacgt attttcggaa  22440 

ctagacgaat tttcggtgga ctcggaaacg cggaagtctc ttcttgtacg gcgttctgaa  22500 

cggccttttg actaaccggc ctgtccggcg cagcacgtgc gtcgtggaac gcagccacaa  22560 

cctctagacg tggtgtaaag ccggggtggc caagaagtgc tagaaccgga acgatctgac  22620 

gaggaagtcg cgcgcgacgg gcaaaagcga gcagtgtagg taaagttagt gcacgaggaa  22680 

taaatagtat tacgaaggca catctgtgaa ttcgagcgga agctagagtc gcgtcgccac  22740 

gtcggtgttg cgcgtcgggc acccgagcac tacgaacatc cagtggagac gtttgctgac  22800 

gtccatgcgg acgtccttag cggggtagta gcagtgtttc cagaacaacg accacttcca  22860 

gtcgacgttg ggcgccacga ggagcaagtc ggtccagaac gtatgccggc ggtctcgaag  22920 

gtgaaccagt ccgtcatcaa acttcaagcg gaaatctagc aataggtgca ccatgaacag  22980 

gtagtcgcgc gcgcgtcgga ggtacgggaa gagggtgcgt ctgtgctagc cgtgtgagtc  23040 

gcccaagtag tggcattaaa gtgaaaggcg aagcgacccg agaaggagaa ggagaacgca  23100 

ggcgtatggt gcgcggtgac ccagcagaag taagtcggcg gcgtgacacg cgaatggagg  23160 

aaacggtacg aactaatcgt ggccacccaa cgactttggg tggtaaacat cgcggtgtag  23220 

aagagaaaga aggagcgaca ggtgctaatg gagaccacta ccgcccgcga gcccgaaccc  23280 

tcttcccgcg aagaaaaaga agaacccgcg ttaccggttt aggcggcggc tccagctacc  23340 

ggcgcccgac ccacacgcgc cgtggtcgcg cagaacacta ctcagaagga gcaggagcct  23400 

gagctatgcg gcggagtagg cgaaaaaacc cccgcgggcc cctccgccgc cgctgcccct  23460 

gcccctgctg tgcaggaggt accaaccccc tgcagcgcgg cgtggcgcag gcgcgagccc  23520 

ccaccaaagc gcgacgagga gaagggctga ccggtaaagg aagaggatat ccgtcttttt  23580 

ctagtacctc agtcagctct tcttcctgtc ggattggcgg gggagactca agcggtggtg  23640 

gcggaggtgg ctacggcggt tgcgcggatg gtggaagggg cagctccgtg ggggcgaact  23700 

cctcctcctt cactaatagc tcgtcctggg tccaaaacat tcgcttctgc tgctcctggc  23760 

gagtcatggt tgtctcctat ttttcgttct ggtcctgttg cgtctccgtt tgctccttgt  23820 

tcagcccgcc cccctgcttt ccgtaccgct gatggatcta caccctctgc tgcacgacaa  23880 

cttcgtagac gtcgcggtca cgcggtaata gacgctgcgc aacgttctcg cgtcgctaca  23940 

cggggagcgg tatcgcctac agtcggaacg gatgcttgcg gtggataaga gtggcgcgca  24000 

tggggggttt gcggttcttt tgccgtgtac gctcgggttg ggcgcggagt tgaagatggg  24060 

gcataaacgg cacggtctcc acgaacggtg gatagtgtag aaaaaggttt tgacgttcta  24120 

tggggatagg acggcacggt tggcgtcggc tcgcctgttc gtcgaccgga acgccgtccc  24180 

gcgacagtat ggactatagc ggagcgagtt gcttcacggt ttttagaaac tcccagaacc  24240 

tgcgctgctc ttcgcgcgcc gtttgcgaga cgttgtcctt ttgtcgcttt tactttcagt  24300 

gagacctcac aaccaccttg agctcccact gttgcgcgcg gatcggcatg attttgcgtc  24360 

gtagctccag tgggtgaaac ggatgggccg tgaattggat ggggggttcc agtactcgtg  24420 

tcagtactca ctcgactagc acgcggcacg cgtcggggac ctctccctac gtttaaacgt  24480 

tcttgtttgt ctcctcccgg atgggcgtca accgctgctc gtcgatcgcg cgaccgaagt  24540 

ttgcgcgctc ggacggctga acctcctcgc tgcgtttgat tactaccggc gtcacgagca  24600 

atggcacctc gaactcacgt acgtcgccaa gaaacgactg ggcctctacg tcgcgttcga  24660 

tctcctttgt aacgtgatgt ggaaagctgt cccgatgcat gcggtccgga cgttctagag  24720 

gttgcacctc gagacgttgg accagaggat ggaaccttaa aacgtgcttt tggcggaacc  24780 

cgttttgcac gaagtaaggt gcgagttccc gctccgcgcg gcgctgatgc aggcgctgac  24840 

gcaaatgaat aaagatacga tgtggaccgt ctgccggtac ccgcaaaccg tcgtcacgaa  24900 

cctcctcacg ttggagttcc tcgacgtctt tgacgatttc gttttgaact tcctggatac  24960 

ctgccggaag ttgctcgcga ggcaccggcg cgtggaccgc ctgtagtaaa aggggcttgc  25020 

ggacgaattt tgggacgttg tcccagacgg tctgaagtgg tcagtttcgt acaacgtctt  25080 

gaaatccttg aaataggatc tcgcgagtcc ttagaacggg cggtggacga cacgtgaagg  25140 

atcgctgaaa cacgggtaat tcatggcgct tacgggaggc ggcgaaaccc cggtgacgat  25200 

ggaagacgtc gatcggttga tggaacggat ggtgagactg tattaccttc tgcactcgcc  25260 

actgccagat gacctcacag tgacagcgac gttggatacg tggggcgtgg cgagggacca  25320 

aacgttaagc gtcgacgaat tgctttcagt ttaatagcca tggaaactcg acgtcccagg  25380 

gagcggactg cttttcaggc gccgaggccc caactttgag tgaggccccg acacctgcag  25440 

ccgaatggaa gcgtttaaac atggactcct gatggtgcgg gtgctctaat ccaagatgct  25500 

tctggttagg gcgggcggat tacgcctcga atggcggacg cagtaatggg tcccggtgta  25560 

agaaccggtt aacgttcggt agttgtttcg ggcggttctc aaagacgatg ctttccctgc  25620 

cccccaaatg aacctggggg tcaggccgct cctcgagttg ggttaggggg gcggcggcgt  25680 

cgggatagtc gtcgtcggcg cccgggaacg aagggtccta ccgtgggttt ttcttcgacg  25740 

tcgacggcgg cggtgggtgc ctgctcctcc ttatgaccct gtcagtccgt ctcctccaaa  25800 

acctgctcct cctcctcctg tactaccttc tgaccctctc ggatctgctc cttcgaaggc  25860 

tccagcttct ccacagtctg ctttgtggca gtgggagcca gcgtaagggg agcggccgcg  25920 

gggtctttag ccgttggcca aggtcgtacc gatgttggag gcgaggagtc cgcggcggcc  25980 

gtgacgggca agcggctggg ttggcatcta ccctgtggtg accttggtcc cggccattca  26040 

ggttcgtcgg cggcggcaat cgggttctcg ttgttgtcgc ggttccgatg gcgagtaccg  26100 

cgcccgtgtt cttgcggtat caacgaacga acgttctgac acccccgttg tagaggaagc  26160 

gggcggcgaa agaagagatg gtagtgccgc accggaaggg ggcattgtag gacgtaatga  26220 

tggcagtaga gatgtcgggt atgacgtggc cgccgtcgcc gtcgttgtcg tcgccggtgt  26280 

gtcttcgttt ccgctggcct atcgttctga gactgtttcg ggttctttag gtgtcgccgc  26340 

cgtcgtcgtc ctcctcctcg cgacgcagac cgcgggttgc ttgggcatag ctgggcgctc  26400 

gaatctttgt cctaaaaagg gtgagacata cgatataaag ttgtctcgtc cccggttctt  26460 

gttctcgact tttatttttt gtccagagac gctagggagt gggcgtcgac ggacatagtg  26520 

ttttcgcttc tagtcgaagc cgcgtgcgac cttctgcgcc tccgagagaa gtcatttatg  26580 

acgcgcgact gagaattcct gatcaaagcg cgggaaagag tttaaattcg cgcttttgat  26640 

gcagtagagg tcgccggtgt gggccgcggt cgtggacaac agtcgcggta atactcgttc  26700 

ctttaagggt gcgggatgta cacctcaatg gtcggtgttt accctgaacg ccgacctcga  26760 

cgggttctga tgagttgggc ttatttgatg tactcgcgcc ctggggtgta ctatagggcc  26820 

cagttgcctt atgcgcgggt ggctttggct taagaggacc ttgtccgccg ataatggtgg  26880 

tgtggagcat tattggaatt aggggcatca accgggcgac gggaccacat ggtcctttca  26940 

gggcgagggt ggtgacacca tgaagggtct ctgcgggtcc ggcttcaagt ctactgattg  27000 

agtccccgcg tcgaacgccc gccgaaagca gtgtcccacg ccagcgggcc cgtcccatat  27060 

tgagtggact gttagtctcc cgctccataa gtcgagttgc tgctcagcca ctcgaggagc  27120 

gaaccagagg caggcctgcc ctgtaaagtc tagccgccgc ggccggcgag aagtaagtgc  27180 

ggagcagtcc gttaggattg agacgtctgg agcaggagac tcggcgcgag acctccgtaa  27240 

ccttgagacg ttaaataact cctcaaacac ggtagccaga tgaaattggg gaagagccct  27300 

ggagggccgg tgataggcct agttaaataa ggattgaaac tgcgccattt cctgagccgc  27360 

ctgccgatgc tgacttacaa ttcacctctc cgtctcgttg acgcggactt tgtggaccag  27420 

gtgacagcgg cggtgttcac gaaacgggcg ctgaggccac tcaaaacgat gaaacttaac  27480 

gggctcctag tatagctccc gggccgcgtg ccgcaggccg aatggcgggt ccctctcgaa  27540 

cgggcatcgg actaagccct caaatgggtc gcgggggacg atcaactcgc cctgtcccct  27600 

gggacacaag agtgacacta aacgttgaca ggattgggac ctaatgtagt tctagaaaca  27660 

acggtagaga cacgactcat attatttatg tctttaattt tatatgaccc cgaggatagc  27720 

ggtaggacat ttgcggtggc agaagtgggc gggttcgttt ggttccgctt ggaatggacc  27780 

atgaaaattg tagagaggga gacactaaat gttgtcaaag ttgggtctgc ctcactcaga  27840 

tgctctcttg gagaggctcg agtcgatgag gtagtctttt ttgtggtggg aggaatggac  27900 

ggcccttgca tgctcacgca gtggccggcg acgtggtgtg gatggcggac tggcatttgg  27960 

tctgaaaaag gcctgtctgg agttattgag acaaatggtc ttgtcctcca ctcgaatctt  28020 

ttgggaatcc cataatccgg tttccgcgtc gatgacaccc caaatacttg ttaagttcgt  28080 

tgagatgccc gataagatta agtccaaaga gatcttagcc ccaaccccaa taagagacag  28140 

aacactaaga gaaataagaa tatgattgcg aagagacgga ttccgagcgg cggacgacac  28200 

acgtgtaaac gtaaataaca gtcgaaaaat ttgcgacccc agcggtgggt tctactaatc  28260 

catgtattag gatccaaatg agtgggaacg cagtcgggtg ccatggtggg ttttccacct  28320 

aaaattcctc ggtcggacat tacaatgtaa gcgtcgactt cgattactca cgtggtgaga  28380 

atattttacg tggtgtcttg tacttttcga cgaataagcg gtgtttttgt tttaaccgtt  28440 

catacgacaa atacgataaa ccgtcggtcc actgtgatgt ctcatattac aatgtcaaaa  28500 

ggtcccattt tcagtatttt gaaaatacat atgaaaaggt aaaatacttt acacgctgta  28560 

atggtacatg tactcgtttg tcatattcaa caccgggggt gttttaacac accttttgtg  28620 

accgtgaaag acgacgtgac gatacgatta atgtcacgag cgaaaccaga catgggatga  28680 

gatataattt atgttttcgt ctgcgtcgaa ataactcctt ttcttttacg gaattaaatg  28740 

attcaatgtt tcgattacag tggtgattga cgaaatgagc gacgaacgtt ttgtttaagt  28800 

ttttcaatcg taatattaat cttatcctaa atttgggggg ccagtaaagg acgagttatg  28860 

gtaaggggac ttgttaactg agatacaccc tatacgaggt cgcgatgttg gaacttcagt  28920 

ccgaaggacc tacagtcgta gactgaaacc ggtcgtggac agggcgccta aacaaggtca  28980 

ggttgatgtc gctgggtggg attgtctcta ctggttgtgt tggttgcgcc ggcggcgatg  29040 

gcctgaatgt agatggtgtt tatgtggggt tcaaagacgg aaacagttat tgaccctatt  29100 

gaacccgtac accaccaaga ggtatcgcga atacaaacat acggaataat aatacaccga  29160 

gtagacgacg gatttcgcgt ttgcgcgggc tggtgggtag atatcagggt agtaacacga  29220 

tgtgggtttg ttactacctt aggtatctaa cctgcctgac tttgtgtaca agaaaagaga  29280 

atgtcatact aatttactct gtactaagga gctcaaaaat ataatgactg ggaacaacgc  29340 

gaaaaaacac gcacgaggtg taaccgacgc caaagagtgt agcttcatct gacgtaaggt  29400 

cggaagtgtc agataaacga aatgcctaaa cagtgggagt gcgagtagac gtcggagtag  29460 

tgacaccagt agcggaaata ggtcacgtaa ctgacccaga cacacgcgaa acgtatagag  29520 

tctgtggtag gggtcatgtc cctgtcctga tatcgactcg aagaatctta agaaattaat  29580 

actttaaatg acactgaaaa gacgactaat aaacgtggga tagacgcaaa acaaggggct  29640 

ggaggttcgg agtttctgta tatagtacgt ctaagtgagc atatacctta taaggttcaa  29700 

cgatgttact tttttcgcta gaaaggcttc ggaccaatat acgttagtag agacaatacc  29760 

acaagacgtc atggtagaat cgggatcgat atatagggat ggaactgtaa ccgaccttgc  29820 

gttatctacg gtacttggtg ggttgaaagg ggcgcgggcg atacgaaggt gacgttgttc  29880 

aacaacggcc gccgaaacag ggtcggttag tcggagcggg tggaagaggg tgggggtgac  29940 

tttagtcgat gaaattagat tgtcctcctc tactgactgt gggatctaga tctttacctg  30000 

ccttaataat gtctcgtcgc ggacgatctt tctgcgtccc gtcgccggct cgttgtcgcg  30060 

tacttagttc tcgaggttct gtaccaattg aacgtggtca cgttttcccc atagaaaaca  30120 

gagcatttcg tccggtttca gtggatgctg tcattatggt ggcctgtggc ggaatcgatg  30180 

ttcaacggtt ggttcgcagt ctttaaccac cagtaccacc ctcttttcgg gtaatggtat  30240 

tgagtcgtga gccatctttg gcttccgacg taagtgagtg gaacagttcc tggactccta  30300 

gagacgtggg aataattctg ggacacgcca gagtttctag aataagggaa attgattatt  30360 

tttttttatt atttcgtagt gaatgaattt tagtcaatcg tttaaagaca ggtcaaataa  30420 

gtcgtcgtgg aggaacggga ggagggtcga gaccataacg tcgaaggagg accgacgttt  30480 

gaaagaggtg ttagatttac cttacagtca aaggaggaca aggacaggta ggcgtgggtg  30540 

atagaagtac aacaacgtct acttcgcgcg ttctggcaga cttctatgga agttggggca  30600 

cataggtata ctgtgccttt ggccaggagg ttgacacgga aaagaatgag gagggaaaca  30660 

tagggggtta cccaaagttc tctcaggggg accccatgag agaaacgcgg ataggcttgg  30720 

agatcaatgg aggttaccgt acgaacgcga gttttacccg ttgccggaga gagacctgct  30780 

ccggccgttg gaatggaggg ttttacattg gtgacactcg ggtggagagt ttttttggtt  30840 

cagtttgtat ttggaccttt atagacgtgg ggagtgtcaa tggagtcttc gggattgaca  30900 

ccgacggcgg cgtggagatt accagcgccc gttgtgtgag tggtacgtta gtgtccgggg  30960 

cgattggcac gtgctgaggt ttgaatcgta acggtgggtt cctggggagt gtcacagtct  31020 

tcctttcgat cgggacgttt gtagtccggg ggagtggtgg tggctatcgt catgggaatg  31080 

atagtgacgg agtgggggaa attgatgacg gtgaccatcg aacccgtaac tgaactttct  31140 

cgggtaaata tgtgttttac cttttgatcc tgatttcatg ccccgaggaa acgtacattg  31200 

tctgctggat ttgtgaaact ggcatcgttg accaggtcca cactgataat tattatgaag  31260 

gaacgtttga tttcaatgac ctcggaaccc aaaactaagt gttccgttat acgttgaatt  31320 

acatcgtcct cctgattcct aactaagagt tttgtctgcg gaatatgaac tacaatcaat  31380 

aggcaaacta cgagttttgg ttgatttaga ttctgatcct gtcccgggag aaaaatattt  31440 

gagtcgggtg ttgaacctat aattgatgtt gtttccggaa atgaacaaat gtcgaagttt  31500 

gttaaggttt ttcgaactcc aattggattc gtgacggttc cccaactaca aactgcgatg  31560 

tcggtatcgg taattacgtc ctctacccga acttaaacca agtggattac gtggtttgtg  31620 

tttaggggag ttttgttttt aaccggtacc ggatcttaaa ctaagtttgt tccgatacca  31680 

aggatttgat ccttgaccgg aatcaaaact gtcgtgtcca cggtaatgtc atcctttgtt  31740 

tttattacta ttcgattgaa acacctggtg tggtcgaggt agaggattga catctgattt  31800 

acgtctcttt ctacgatttg agtgaaacca gaattgtttt acaccgtcag tttatgaacg  31860 

atgtcaaagt caaaaccgac aatttccgtc aaaccgaggt tatagacctt gtcaagtttc  31920 

acgagtagaa taatattcta aactgctttt acctcacgat gatttgttaa ggaaggacct  31980 

gggtcttata accttgaaat ctttacctct agaatgactt ccgtgtcgga tatgtttgcg  32040 

acaacctaaa tacggattgg atagtcgaat aggttttaga gtgccatttt gacggttttc  32100 

attgtaacag tcagttcaaa tgaatttgcc tctgttttga tttggacatt gtgattggta  32160 

atgtgatttg ccatgtgtcc tttgtcctct gtgttgaggt tcacgtatga gatacagtaa  32220 

aagtaccctg accagaccgg tgttgatgta attactttat aaacggtgta ggagaatgtg  32280 

aaaaagtatg taacgggttc ttatttctta gcaaacacaa tacaaagttg cacaaataaa  32340 

aagttaacgt cttttaaagt tcagtaaaaa gtaagtcatc atatcggggt ggtggtgtat  32400 

cgaatatgtc tagtggcatg gaattagttt gagtgtcttg ggatcataag ttggacggtg  32460 

gagggagggt tgtgtgtctc atgtgtcagg aaagaggggc cgaccggaat ttttcgtagt  32520 

atagtaccca ttgtctgtat aagaatccac aatataaggt gtgccaaagg acagctcggt  32580 

ttgcgagtag tcactataat tatttgaggg gcccgtcgag tgaattcaag tacagcgaca  32640 

ggtcgacgac tcggtgtccg acgacaggtt gaacgccaac gaattgcccg ccgcttcctc  32700 

ttcaggtgcg gatgtacccc catctcagta ttagcacgta gtcctatccc gccaccacga  32760 

cgtcgtcgcg cgcttatttg acgacggcgg cggcgaggca ggacgtcctt atgttgtacc  32820 

gtcaccagag gagtcgctac taagcgtggc gggcgtcgta ttccgcggaa caggaggccc  32880 

gtgtcgtcgc gtgggactag agtgaattta gtcgtgtcat tgacgtcgtg tcgtggtgtt  32940 

ataacaagtt ttagggtgtc acgttccgcg acataggttt cgagtaccgc ccctggtgtc  33000 

ttgggtgcac cggtagtatg gtgttcgcgt ccatctaatt caccgctggg gagtatttgt  33060 

gcgacctgta tttgtaatgg agaaaaccgt acaacattaa gtggtggagg gccatggtat  33120 

atttggagac taatttgtac cgcggtaggt ggtggtagga tttggtcgac cggttttgga  33180 

cgggcggccg atatgtgacg tcccttggcc ctgaccttgt tactgtcacc tctcgggtcc  33240 

tgagcattgg tacctagtag tacgagcagt actatagtta caaccgtgtt gtgtccgtgt  33300 

gcacgtatgt gaaggagtcc taatgttcga ggagggcgca atcttggtat agggtccctt  33360 

gttgggtaag gacttagtcg catttagggt gtgacgtccc ttctggagcg tgcattgagt  33420 

gcaacacgta acagtttcac aatgtaagcc cgtcgtcgcc tactaggagg tcataccatc  33480 

gcgcccaaag acagagtttt cctccatctg ctagggatga catgcctcac gcggctctgt  33540 

tggctctagc acaaccagca tcacagtacg gtttaccttg cggcctgcat cagtataaag  33600 

gacttcgttt tggtccacgc ccgcactgtt tgtctagacg cagaggccag agcggcgaat  33660 

ctagcgagac acatcatcaa catcatatag gtgagagagt ttcgtaggtc cgcgggggac  33720 

cgaagcccaa gatacatttg aggaagtacg cggcgacggg actattgtag gtggtggcgt  33780 

cttattcggt gtgggtcggt tggatgtgta agcaagacgc tcagtgtgtg ccctcctcgc  33840 

ccttctcgac cttcttggta caaaaaaaaa aataaggttt tctaataggt tttggagttt  33900 

tacttctaga taattcactt gcgcgagggg aggccaccgc accagtttga gatgtcggtt  33960 

tcttgtctat taccgtaaac attctacaac gtgttaccga aggttttccg tttgccggga  34020 

gtgcaggttc acctgcattt ccgatttggg aagtcccact tagaggagat atttgtaagg  34080 

tcgtggaagt tggtacgggt ttattaagag tagagcggtg gaagagttat atagagattc  34140 

gtttagggct tataattcag gccggtaaca tttttagacg aggtctcgcg ggaggtggaa  34200 

gtcggagttc gtcgcttagt actaacgttt ttaagtccaa ggagtgtctg gacatattct  34260 

aagttttcgc cttgtaattg tttttatggc gctagggcat ccagggaagc gtcccggtcg  34320 

acttgtatta gcacgtccag acgtgcctgg tcgcgccggt gaaggggcgg tccttggtac  34380 

tgttttcttg ggtgtgacta atactgtgcg tatgagcctc gatacgattg gtcgcatcgg  34440 

ggctacattc gaacaacgta cccgccgcta tattttacgt tccacgacga gttttttagt  34500 

ccgtttcgga gcgcgttttt tctttcgtgt agcatcagta cgagtacgtc tatttccgtc  34560 

cattcgaggc cttggtggtg tctttttctg tggtaaaaag agagtttgta cagacgccca  34620 

aagacgtatt tgtgttttat tttattgttt ttttgtaaat ttgtaatctt cggacagaat  34680 

gttgtccttt ttgttgggaa tattcgtatt ctgcctgatg ccggtacggc cgcactggca  34740 

tttttttgac cagtggcact aatttttcgt ggtggctgtc gaggagccag tacaggcctc  34800 

agtattacat tctgagccat ttgtgtagtc caactaagtg tagccagtca cgatttttcg  34860 

ctggctttat cgggccccct tatgtatggg cgtccgcatc tctgttgtaa tgtcgggggt  34920 

atcctccata ttgttttaat tatcctctct ttttgtgtat ttgtggactt tttgggagga  34980 

cggatccgtt ttatcgtggg agggcgaggt cttgttgtat gtcgcgaagg tgtcgccgtc  35040 

ggtattgtca gtcggaatgg tcattttttc ttttggataa tttttttgtg gtgagctgtg  35100 

ccgtggtcga gttagtcagt gtcacatttt ttcccggttc acgtctcgct catatatatc  35160 

ctgatttttt actgcattgc caatttcagg tgttttttgt gggtcttttg gcgtgcgctt  35220 

ggatgcgggt ctttgctttc ggttttttgg gtgttgaagg agtttagcag tgaaggcaaa  35280 

agggtgcaat gcagtgaagg gtaaaattct tttgatgtta agggttgtgt atgttcaatg  35340 

aggcgggatt ttggatgcag tgggcggggc aagggtgcgg ggcgcggtgc agtgtttgag  35400 

gtgggggagt aatagtataa ccgaagttag gttttattcc atataataac tactacaatt  35460 

aattcttaag cctagacgct gcgctccgac ctaccggaag gggtaatact aagaagagcg  35520 

aaggccgccg tagccctacg ggcgcaacgt ccggtacgac aggtccgtcc atctactgct  35580 

ggtagtccct gtcgaagttc cggtcgtttt ccggtccttg gcatttttcc ggcgcaacga  35640 

ccgcaaaaag gtatccgagg cggggggact gctcgtagtg tttttagctg cgagttcagt  35700 

ctccaccgct ttgggctgtc ctgatatttc tatggtccgc aaagggggac cttcgaggga  35760 

gcacgcgaga ggacaaggct gggacggcga atggcctatg gacaggcgga aagagggaag  35820 

cccttcgcac cgcgaaagag tatcgagtgc gacatccata gagtcaagcc acatccagca  35880 

agcgaggttc gacccgacac acgtgcttgg ggggcaagtc gggctggcga cgcggaatag  35940 

gccattgata gcagaactca ggttgggcca ttctgtgctg aatagcggtg accgtcgtcg  36000 

gtgaccattg tcctaatcgt ctcgctccat acatccgcca cgatgtctca agaacttcac  36060 

caccggattg atgccgatgt gatcttcctg tcataaacca tagacgcgag acgacttcgg  36120 

tcaatggaag cctttttctc aaccatcgag aactaggccg tttgtttggt ggcgaccatc  36180 

gccaccaaaa aaacaaacgt tcgtcgtcta atgcgcgtct ttttttccta gagttcttct  36240 

aggaaactag aaaagatgcc ccagactgcg agtcaccttg cttttgagtg caattcccta  36300 

aaaccagtac tctaatagtt tttcctagaa gtggatctag gaaaatttag ttagatttca  36360 

tatatactca tttgaaccag actgtcaatg gttacgaatt agtcactccg tggatagagt  36420 

cgctagacag ataaagcaag taggtatcaa cggactgagg ggcagcacat ctattgatgc  36480 

tatgccctcc cgaatggtag accggggtca cgacgttact atggcgctct gggtgcgagt  36540 

ggccgaggtc taaatagtcg ttatttggtc ggtcggcctt cccggctcgc gtcttcacca  36600 

ggacgttgaa ataggcggag gtaggtcaga taattaacaa cggcccttcg atctcattca  36660 

tcaagcggtc aattatcaaa cgcgttgcaa caacggtaac gatgtccgta gcaccacagt  36720 

gcgagcagca aaccataccg aagtaagtcg aggccaaggg ttgctagttc cgctcaatgt  36780 

actagggggt acaacacgtt ttttcgccaa tcgaggaagc caggaggcta gcaacagtct  36840 

tcattcaacc ggcgtcacaa tagtgagtac caataccgtc gtgacgtatt aagagaatga  36900 

cagtacggta ggcattctac gaaaagacac tgaccactca tgagttggtt cagtaagact  36960 

cttatcacat acgccgctgg ctcaacgaga acgggccgca gttgtgccct attatggcgc  37020 

ggtgtatcgt cttgaaattt tcacgagtag taaccttttg caagaagccc cgcttttgag  37080 

agttcctaga atggcgacaa ctctaggtca agctacattg ggtgagcacg tgggttgact  37140 

agaagtcgta gaaaatgaaa gtggtcgcaa agacccactc gtttttgtcc ttccgtttta  37200 

cggcgttttt tcccttattc ccgctgtgcc tttacaactt atgagtatga gaaggaaaaa  37260 

gttataataa cttcgtaaat agtcccaata acagagtact cgcctatgta taaacttaca  37320 

taaatctttt tatttgttta tccccaaggc gcgtgtaaag gggcttttca cggtggactg  37380 

cagattcttt ggtaataata gtactgtaat tggatatttt tatccgcata gtgctccggg  37440 

aaagcagaag ttcttaacct aggcttaaga atta                              37474 

 
           
             9  
             35759  
             DNA  
             Artificial Sequence  
             
               Ad6 genome  
             
           
            9 

catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt     60 

ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt    120 

gatgttgtaa gtgtggcgga acacatgtaa gcgccggatg tggtaaaagt gacgtttttg    180 

gtgtgcgccg gtgtacacgg gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag    240 

taaatttggg cgtaaccaag taatatttgg ccattttcgc gggaaaactg aataagagga    300 

agtgaaatct gaataattct gtgttactca tagcgcgtaa tatttgtcta gggccgcggg    360 

gactttgacc gtttacgtgg agactcgccc aggtgttttt ctcaggtgtt ttccgcgttc    420 

cgggtcaaag ttggcgtttt attattatag tcagctgacg cgcagtgtat ttatacccgg    480 

tgagttcctc aagaggccac tcttgagtgc cagcgagtag agttttctcc tccgagccgc    540 

tccgacaccg ggactgaaaa tgagacatat tatctgccac ggaggtgtta ttaccgaaga    600 

aatggccgcc agtcttttgg accagctgat cgaagaggta ctggctgata atcttccacc    660 

tcctagccat tttgaaccac ctacccttca cgaactgtat gatttagacg tgacggcccc    720 

cgaagatccc aacgaggagg cggtttcgca gatttttccc gagtctgtaa tgttggcggt    780 

gcaggaaggg attgacttat tcacttttcc gccggcgccc ggttctccgg agccgcctca    840 

cctttcccgg cagcccgagc agccggagca gagagccttg ggtccggttt ctatgccaaa    900 

ccttgtgccg gaggtgatcg atcttacctg ccacgaggct ggctttccac ccagtgacga    960 

cgaggatgaa gagggtgagg agtttgtgtt agattatgtg gagcaccccg ggcacggttg   1020 

caggtcttgt cattatcacc ggaggaatac gggggaccca gatattatgt gttcgctttg   1080 

ctatatgagg acctgtggca tgtttgtcta cagtaagtga aaaattatgg gcagtgggtg   1140 

atagagtggt gggtttggtg tggtaatttt ttttttaatt tttacagttt tgtggtttaa   1200 

agaattttgt attgtgattt tttaaaaggt cctgtgtctg aacctgagcc tgagcccgag   1260 

ccagaaccgg agcctgcaag acctacccgg cgtcctaaat tggtgcctgc tatcctgaga   1320 

cgcccgacat cacctgtgtc tagagaatgc aatagtagta cggatagctg tgactccggt   1380 

ccttctaaca cacctcctga gatacacccg gtggtcccgc tgtgccccat taaaccagtt   1440 

gccgtgagag ttggtgggcg tcgccaggct gtggaatgta tcgaggactt gcttaacgag   1500 

tctgggcaac ctttggactt gagctgtaaa cgccccaggc cataaggtgt aaacctgtga   1560 

ttgcgtgtgt ggttaacgcc tttgtttgct gaatgagttg atgtaagttt aataaagggt   1620 

gagataatgt ttaacttgca tggcgtgtta aatggggcgg ggcttaaagg gtatataatg   1680 

cgccgtgggc taatcttggt tacatctgac ctcatggagg cttgggagtg tttggaagat   1740 

ttttctgctg tgcgtaactt gctggaacag agctctaaca gtacctcttg gttttggagg   1800 

tttctgtggg gctcctccca ggcaaagtta gtctgcagaa ttaaggagga ttacaagtgg   1860 

gaatttgaag agcttttgaa atcctgtggt gagctgtttg attctttgaa tctgggtcac   1920 

caggcgcttt tccaagagaa ggtcatcaag actttggatt tttccacacc ggggcgcgct   1980 

gcggctgctg ttgctttttt gagttttata aaggataaat ggagcgaaga aacccatctg   2040 

agcggggggt acctgctgga ttttctggcc atgcatctgt ggagagcggt ggtgagacac   2100 

aagaatcgcc tgctactgtt gtcttccgtc cgcccggcaa taataccgac ggaggagcaa   2160 

cagcaggagg aagccaggcg gcggcggcgg caggagcaga gcccatggaa cccgagagcc   2220 

ggcctggacc ctcgggaatg aatgttgtac aggtggctga actgtttcca gaactgagac   2280 

gcattttaac cattaacgag gatgggcagg ggctaaaggg ggtaaagaag gagcgggggg   2340 

cttctgaggc tacagaggag gctaggaatc taacttttag cttaatgacc agacaccgtc   2400 

ctgagtgtgt tacttttcag cagattaagg ataattgcgc taatgagctt gatctgctgg   2460 

cgcagaagta ttccatagag cagctgacca cttactggct gcagccaggg gatgattttg   2520 

aggaggctat tagggtatat gcaaaggtgg cacttaggcc agattgcaag tacaagatta   2580 

gcaaacttgt aaatatcagg aattgttgct acatttctgg gaacggggcc gaggtggaga   2640 

tagatacgga ggatagggtg gcctttagat gtagcatgat aaatatgtgg ccgggggtgc   2700 

ttggcatgga cggggtggtt attatgaatg tgaggtttac tggtcccaat tttagcggta   2760 

cggttttcct ggccaatacc aatcttatcc tacacggtgt aagcttctat gggtttaaca   2820 

atacctgtgt ggaagcctgg accgatgtaa gggttcgggg ctgtgccttt tactgctgct   2880 

ggaagggggt ggtgtgtcgc cccaaaagca gggcttcaat taagaaatgc ctgtttgaaa   2940 

ggtgtacctt gggtatcctg tctgagggta actccagggt gcgccacaat gtggcctccg   3000 

actgtggttg ctttatgcta gtgaaaagcg tggctgtgat taagcataac atggtgtgtg   3060 

gcaactgcga ggacagggcc tctcagatgc tgacctgctc ggacggcaac tgtcacttgc   3120 

tgaagaccat tcacgtagcc agccactctc gcaaggcctg gccagtgttt gagcacaaca   3180 

tactgacccg ctgttccttg catttgggta acaggagggg ggtgttccta ccttaccaat   3240 

gcaatttgag tcacactaag atattgcttg agcccgagag catgtccaag gtgaacctga   3300 

acggggtgtt tgacatgacc atgaagatct ggaaggtgct gaggtacgat gagacccgca   3360 

ccaggtgcag accctgcgag tgtggcggta aacatattag gaaccagcct gtgatgctgg   3420 

atgtgaccga ggagctgagg cccgatcact tggtgctggc ctgcacccgc gctgagtttg   3480 

gctctagcga tgaagataca gattgaggta ctgaaatgtg tgggcgtggc ttaagggtgg   3540 

gaaagaatat ataaggtggg ggtctcatgt agttttgtat ctgttttgca gcagccgccg   3600 

ccatgagcgc caactcgttt gatggaagca ttgtgagctc atatttgaca acgcgcatgc   3660 

ccccatgggc cggggtgcgt cagaatgtga tgggctccag cattgatggt cgccccgtcc   3720 

tgcccgcaaa ctctactacc ttgacctacg agaccgtgtc tggaacgccg ttggagactg   3780 

cagcctccgc cgccgcttca gccgctgcag ccaccgcccg cgggattgtg actgactttg   3840 

ctttcctgag cccgcttgca agcagtgcag cttcccgttc atccgcccgc gatgacaagt   3900 

tgacggctct tttggcacaa ttggattctt tgacccggga acttaatgtc gtttctcagc   3960 

agctgttgga tctgcgccag caggtttctg ccctgaaggc ttcctcccct cccaatgcgg   4020 

tttaaaacat aaataaaaac cagactctgt ttggatttgg atcaagcaag tgtcttgctg   4080 

tctttattta ggggttttgc gcgcgcggta ggcccgggac cagcggtctc ggtcgttgag   4140 

ggtcctgtgt attttttcca ggacgtggta aaggtgactc tggatgttca gatacatggg   4200 

cataagcccg tctctggggt ggaggtagca ccactgcaga gcttcatgct gcggggtggt   4260 

gttgtagatg atccagtcgt agcaggagcg ctgggcgtgg tgcctaaaaa tgtctttcag   4320 

tagcaagctg attgccaggg gcaggccctt ggtgtaagtg tttacaaagc ggttaagctg   4380 

ggatgggtgc atacgtgggg atatgagatg catcttggac tgtattttta ggttggctat   4440 

gttcccagcc atatccctcc ggggattcat gttgtgcaga accaccagca cagtgtatcc   4500 

ggtgcacttg ggaaatttgt catgtagctt agaaggaaat gcgtggaaga acttggagac   4560 

gcccttgtga cctccaagat tttccatgca ttcgtccata atgatggcaa tgggcccacg   4620 

ggcggcggcc tgggcgaaga tatttctggg atcactaacg tcatagttgt gttccaggat   4680 

gagatcgtca taggccattt ttacaaagcg cgggcggagg gtgccagact gcggtataat   4740 

ggttccatcc ggcccagggg cgtagttacc ctcacagatt tgcatttccc acgctttgag   4800 

ttcagatggg gggatcatgt ctacctgcgg ggcgatgaag aaaaccgttt ccggggtagg   4860 

ggagatcagc tgggaagaaa gcaggttcct aagcagctgc gacttaccgc agccggtggg   4920 

cccgtaaatc acacctatta ccggctgcaa ctggtagtta agagagctgc agctgccgtc   4980 

atccctgagc aggggggcca cttcgttaag catgtccctg acttgcatgt tttccctgac   5040 

caaatccgcc agaaggcgct cgccgcccag cgatagcagt tcttgcaagg aagcaaagtt   5100 

tttcaacggt ttgaggccgt ccgccgtagg catgcttttg agcgtttgac caagcagttc   5160 

caggcggtcc cacagctcgg tcacgtgctc tacggcatct cgatccagca tatctcctcg   5220 

tttcgcgggt tggggcggct ttcgctgtac ggcagtagtc ggtgctcgtc cagacgggcc   5280 

agggtcatgt ctttccacgg gcgcagggtc ctcgtcagcg tagtctgggt cacggtgaag   5340 

gggtgcgctc cgggttgcgc gctggccagg gtgcgcttga ggctggtcct gctggtgctg   5400 

aagcgctgcc ggtcttcgcc ctgcgcgtcg gccaggtagc atttgaccat ggtgtcatag   5460 

tccagcccct ccgcggcgtg gcccttggcg cgcagcttgc ccttggagga ggcgccgcac   5520 

gaggggcagt gcagactttt aagggcgtag agcttgggcg cgagaaatac cgattccggg   5580 

gagtaggcat ccgcgccgca ggccccgcag acggtctcgc attccacgag ccaggtgagc   5640 

tctggccgtt cggggtcaaa aaccaggttt cccccatgct ttttgatgcg tttcttacct   5700 

ctggtttcca tgagccggtg tccacgctcg gtgacgaaaa ggctgtccgt gtccccgtat   5760 

acagacttga gaggcctgtc ctcgagcggt gttccgcggt cctcctcgta tagaaactcg   5820 

gaccactctg agacgaaggc tcgcgtccag gccagcacga aggaggctaa gtgggagggg   5880 

tagcggtcgt tgtccactag ggggtccact cgctccaggg tgtgaagaca catgtcgccc   5940 

tcttcggcat caaggaaggt gattggttta taggtgtagg ccacgtgacc gggtgttcct   6000 

gaaggggggc tataaaaggg ggtgggggcg cgttcgtcct cactctcttc cgcatcgctg   6060 

tctgcgaggg ccagctgttg gggtgagtac tccctctcaa aagcgggcat gacttctgcg   6120 

ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc cgcggtgatg   6180 

cctttgaggg tggccgcgtc catctggtca gaaaagacaa tctttttgtt gtcaagcttg   6240 

gtggcaaacg acccgtagag ggcgttggac agcaacttgg cgatggagcg cagggtttgg   6300 

tttttgtcgc gatcggcgcg ctccttggcc gcgatgttta gctgcacgta ttcgcgcgca   6360 

acgcaccgcc attcgggaaa gacggtggtg cgctcgtcgg gcactaggtg cacgcgccaa   6420 

ccgcggttgt gcagggtgac aaggtcaacg ctggtggcta cctctccgcg taggcgctcg   6480 

ttggtccagc agaggcggcc gcccttgcgc gagcagaatg gcggtagtgg gtctagctgc   6540 

gtctcgtccg gggggtctgc gtccacggta aagaccccgg gcagcaggcg cgcgtcgaag   6600 

tagtctatct tgcatccttg caagtctagc gcctgctgcc atgcgcgggc ggcaagcgcg   6660 

cgctcgtatg ggttgagtgg gggaccccat ggcatggggt gggtgagcgc ggaggcgtac   6720 

atgccgcaaa tgtcgtaaac gtagaggggc tctctgagta ttccaagata tgtagggtag   6780 

catcttccac cgcggatgct ggcgcgcacg taatcgtata gttcgtgcga gggagcgagg   6840 

aggtcgggac cgaggttgct acgggcgggc tgctctgctc ggaagactat ctgcctgaag   6900 

atggcatgtg agttggatga tatggttgga cgctggaaga cgttgaagct ggcgtctgtg   6960 

agacctaccg cgtcacgcac gaaggaggcg taggagtcgc gcagcttgtt gaccagctcg   7020 

gcggtgacct gcacgtctag ggcgcagtag tccagggttt ccttgatgat gtcatactta   7080 

tcctgtccct tttttttcca cagctcgcgg ttgaggacaa actcttcgcg gtctttccag   7140 

tactcttgga tcggaaaccc gtcggcctcc gaacggtaag agcctagcat gtagaactgg   7200 

ttgacggcct ggtaggcgca gcatcccttt tctacgggta gcgcgtatgc ctgcgcggcc   7260 

ttccggagcg aggtgtgggt gagcgcaaag gtgtccctaa ccatgacttt gaggtactgg   7320 

tatttgaagt cagtgtcgtc gcatccgccc tgctcccaga gcaaaaagtc cgtgcgcttt   7380 

ttggaacgcg ggtttggcag ggcgaaggtg acatcgttga agagtatctt tcccgcgcga   7440 

ggcataaagt tgcgtgtgat gcggaagggt cccggcacct cggaacggtt gttaattacc   7500 

tgggcggcga gcacgatctc gtcaaagccg ttgatgttgt ggcccacaat gtaaagttcc   7560 

aagaagcgcg ggatgccctt gatggaaggc aattttttaa gttcctcgta ggtgagctct   7620 

tcaggggagc tgagcccgtg ctctgaaagg gcccagtctg caagatgagg gttggaagcg   7680 

acgaatgagc tccacaggtc acgggccatt agcatttgca ggtggtcgcg aaaggtccta   7740 

aactggcgac ctatggccat tttttctggg gtgatgcagt agaaggtaag cgggtcttgt   7800 

tcccagcggt cccatccaag gtccgcggct aggtctcgcg cggcggtcac tagaggctca   7860 

tctccgccga acttcatgac cagcatgaag ggcacgagct gcttcccaaa ggcccccatc   7920 

caagtatagg tctctacatc gtaggtgaca aagagacgct cggtgcgagg atgcgagccg   7980 

atcgggaaga actggatctc ccgccaccag ttggaggagt ggctgttgat gtggtgaaag   8040 

tagaagtccc tgcgacgggc cgaacactcg tgctggcttt tgtaaaaacg tgcgcagtac   8100 

tggcagcggt gcacgggctg tacatcctgc acgaggttga cctgacgacc gcgcacaagg   8160 

aagcagagtg ggaatttgag cccctcgcct ggcgggtttg gctggtggtc ttctacttcg   8220 

gctgcttgtc cttgaccgtc tggctgctcg aggggagtta cggtggatcg gaccaccacg   8280 

ccgcgcgagc ccaaagtcca gatgtccgcg cgcggcggtc ggagcttgat gacaacatcg   8340 

cgcagatggg agctgtccat ggtctggagc tcccgcggcg tcaggtcagg cgggagctcc   8400 

tgcaggttta cctcgcatag ccgggtcagg gcgcgggcta ggtccaggtg atacctgatt   8460 

tccaggggct ggttggtggc ggcgtcgatg gcttgcaaga ggccgcatcc ccgcggcgcg   8520 

actacggtac cgcgcggcgg gcggtgggcc gcgggggtgt ccttggatga tgcatctaaa   8580 

agcggtgacg cgggcgggcc cccggaggta gggggggctc gggacccgcc gggagagggg   8640 

gcaggggcac gtcggcgccg cgcgcgggca ggagctggtg ctgcgcgcgg aggttgctgg   8700 

cgaacgcgac gacgcggcgg ttgatctcct gaatctggcg cctctgcgtg aagacgacgg   8760 

gcccggtgag cttgaacctg aaagagagtt cgacagaatc aatttcggtg tcgttgacgg   8820 

cggcctggcg caaaatctcc tgcacgtctc ctgagttgtc ttgataggcg atctcggcca   8880 

tgaactgctc gatctcttcc tcctggagat ctccgcgtcc ggctcgctcc acggtggcgg   8940 

cgaggtcgtt ggagatgcgg gccatgagct gcgagaaggc gttgaggcct ccctcgttcc   9000 

agacgcggct gtagaccacg cccccttcgg catcgcgggc gcgcatgacc acctgcgcga   9060 

gattgagctc cacgtgccgg gcgaagacgg cgtagtttcg caggcgctga aagaggtagt   9120 

tgagggtggt ggcggtgtgt tctgccacga agaagtacat aacccagcgc cgcaacgtgg   9180 

attcgttgat atcccccaag gcctcaaggc gctccatggc ctcgtagaag tccacggcga   9240 

agttgaaaaa ctgggagttg cgcgccgaca cggttaactc ctcctccaga agacggatga   9300 

gctcggcgac agtgtcgcgc acctcgcgct caaaggctac aggggcctct tcttcttctt   9360 

caatctcctc ttccataagg gcctcccctt cttcttcttc tggcggcggt gggggagggg   9420 

ggacacggcg gcgacgacgg cgcaccggga ggcggtcgac aaagcgctcg atcatctccc   9480 

cgcggcgacg gcgcatggtc tcggtgacgg cgcggccgtt ctcgcggggg cgcagttgga   9540 

agacgccgcc cgtcatgtcc cggttatggg ttggcggggg gctgccgtgc ggcagggata   9600 

cggcgctaac gatgcatctc aacaattgtt gtgtaggtac tccgccaccg agggacctga   9660 

gcgagtccgc atcgaccgga tcggaaaacc tctcgagaaa ggcgtctaac cagtcacagt   9720 

cgcaaggtag gctgagcacc gtggcgggcg gcagcgggcg gcggtcgggg ttgtttctgg   9780 

cggaggtgct gctgatgatg taattaaagt aggcggtctt gagacggcgg atggtcgaca   9840 

gaagcaccat gtccttgggt ccggcctgct gaatgcgcag gcggtcggcc atgccccagg   9900 

cttcgttttg acatcggcgc aggtctttgt agtagtcttg catgagcctt tctaccggca   9960 

cttcttcttc tccttcctct tgtcctgcat ctcttgcatc tatcgctgcg gcggcggcgg  10020 

agtttggccg taggtggcgc cctcttcctc ccatgcgtgt gaccccgaag cccctcatcg  10080 

gctgaagcag ggccaggtcg gcgacaacgc gctcggctaa tatggcctgc tgcacctgcg  10140 

tgagggtaga ctggaagtcg tccatgtcca caaagcggtg gtatgcgccc gtgttgatgg  10200 

tgtaagtgca gttggccata acggaccagt taacggtctg gtgacccggc tgcgagagct  10260 

cggtgtacct gagacgcgag taagcccttg agtcaaagac gtagtcgttg caagtccgca  10320 

ccaggtactg gtatcccacc aaaaagtgcg gcggcggctg gcggtagagg ggccagcgta  10380 

gggtggccgg ggctccgggg gcgaggtctt ccaacataag gcgatgatat ccgtagatgt  10440 

acctggacat ccaggtgatg ccggcggcgg tggtggaggc gcgcggaaag tcacggacgc  10500 

ggttccagat gttgcgcagc ggcaaaaagt gctccatggt cgggacgctc tggccggtca  10560 

ggcgcgcgca gtcgttgacg ctctagaccg tgcaaaagga gagcctgtaa gcgggcactc  10620 

ttccgtggtc tggtggataa attcgcaagg gtatcatggc ggacgaccgg ggttcgaacc  10680 

ccggatccgg ccgtccgccg tgatccatgc ggttaccgcc cgcgtgtcga acccaggtgt  10740 

gcgacgtcag acaacggggg agcgctcctt ttggcttcct tccaggcgcg gcggatgctg  10800 

cgctagcttt tttggccact ggccgcgcgc ggcgtaagcg gttaggctgg aaagcgaaag  10860 

cattaagtgg ctcgctccct gtagccggag ggttattttc caagggttga gtcgcgggac  10920 

ccccggttcg agtctcgggc cggccggact gcggcgaacg ggggtttgcc tccccgtcat  10980 

gcaagacccc gcttgcaaat tcctccggaa acagggacga gccccttttt tgcttttccc  11040 

agatgcatcc ggtgctgcgg cagatgcgcc cccctcctca gcagcggcaa gagcaagagc  11100 

agcggcagac atgcagggca ccctcccctt ctcctaccgc gtcaggaggg gcaacatccg  11160 

cggctgacgc ggcggcagat ggtgattacg aacccccgcg gcgccggacc cggcactact  11220 

tggacttgga ggagggcgag ggcctggcgc ggctaggagc gccctctcct gagcgacacc  11280 

caagggtgca gctgaagcgt gacacgcgcg aggcgtacgt gccgcggcag aacctgtttc  11340 

gcgaccgcga gggagaggag cccgaggaga tgcgggatcg aaagttccat gcagggcgcg  11400 

agttgcggca tggcctgaac cgcgagcggt tgctgcgcga ggaggacttt gagcccgacg  11460 

cgcggaccgg gattagtccc gcgcgcgcac acgtggcggc cgccgacctg gtaaccgcgt  11520 

acgagcagac ggtgaaccag gagattaact ttcaaaaaag ctttaacaac cacgtgcgca  11580 

cgcttgtggc gcgcgaggag gtggctatag gactgatgca tctgtgggac tttgtaagcg  11640 

cgctggagca aaacccaaat agcaagccgc tcatggcgca gctgttcctt atagtgcagc  11700 

acagcaggga caacgaggca ttcagggatg cgctgctaaa catagtagag cccgagggcc  11760 

gctggctgct cgatttgata aacattctgc agagcatagt ggtgcaggag cgcagcttga  11820 

gcctggctga caaggtggcc gccattaact attccatgct cagtctgggc aagttttacg  11880 

cccgcaagat ataccatacc ccttacgttc ccatagacaa ggaggtaaag atcgaggggt  11940 

tctacatgcg catggcgctg aaggtgctta ccttgagcga cgacctgggc gtttatcgca  12000 

acgagcgcat ccacaaggcc gtgagcgtga gccggcggcg cgagctcagc gaccgcgagc  12060 

tgatgcacag cctgcaaagg gccctggctg gcacgggcag cggcgataga gaggccgagt  12120 

cctactttga cgcgggcgct gacctgcgct gggccccaag ccgacgcgcc ctggaggcag  12180 

ctggggccgg acctgggctg gcggtggcac ccgcgcgcgc tggcaacgtc ggcggcgtgg  12240 

aggaatatga cgaggacgat gagtacgagc cagaggacgg cgagtactaa gcggtgatgt  12300 

ttctgatcag atgatgcaag acgcaacgga cccggcggtg cgggcggcgc tgcagagcca  12360 

gccgtccggc cttaactcca cggacgactg gcgccaggtc atggaccgca tcatgtcgct  12420 

gactgcgcgc aaccctgacg cgttccggca gcagccgcag gccaaccggc tctccgcaat  12480 

tctggaagcg gtggtcccgg cgcgcgcaaa ccccacgcac gagaaggtgc tggcgatcgt  12540 

aaacgcgctg gccgaaaaca gggccatccg gcccgatgag gccggcctgg tctacgacgc  12600 

gctgcttcag cgcgtggctc gttacaacag cagcaacgtg cagaccaacc tggaccggct  12660 

ggtgggggat gtgcgcgagg ccgtggcgca gcgtgagcgc gcgcagcagc agggcaacct  12720 

gggctccatg gttgcactaa acgccttcct gagtacacag cccgccaacg tgccgcgggg  12780 

acaggaggac tacaccaact ttgtgagcgc actgcggcta atggtgactg agacaccgca  12840 

aagtgaggtg tatcagtccg ggccagacta ttttttccag accagtagac aaggcctgca  12900 

gaccgtaaac ctgagccagg ctttcaagaa cttgcagggg ctgtgggggg tgcgggctcc  12960 

cacaggcgac cgcgcgaccg tgtctagctt gctgacgccc aactcgcgcc tgttgctgct  13020 

gctaatagcg cccttcacgg acagtggcag cgtgtcccgg gacacatacc taggtcactt  13080 

gctgacactg taccgcgagg ccataggtca ggcgcatgtg gacgagcata ctttccagga  13140 

gattacaagt gttagccgcg cgctggggca ggaggacacg ggcagcctgg aggcaaccct  13200 

gaactacctg ctgaccaacc ggcggcaaaa aatcccctcg ttgcacagtt taaacagcga  13260 

ggaggagcgc attttgcgct atgtgcagca gagcgtgagc cttaacctga tgcgcgacgg  13320 

ggtaacgccc agcgtggcgc tggacatgac cgcgcgcaac atggaaccgg gcatgtatgc  13380 

ctcaaaccgg ccgtttatca atcgcctaat ggactacttg catcgcgcgg ccgccgtgaa  13440 

ccccgagtat ttcaccaatg ccatcttgaa cccgcactgg ctaccgcccc ctggtttcta  13500 

caccggggga ttcgaggtgc ccgagggtaa cgatggattc ctctgggacg acatagacga  13560 

cagcgtgttt tccccgcaac cgcagaccct gctagagttg caacaacgcg agcaggcaga  13620 

ggcggcgctg cgaaaggaaa gcttccgcag gccaagcagc ttgtccgatc taggcgctgc  13680 

ggccccgcgg tcagatgcta gtagcccatt tccaagcttg atagggtctc ttaccagcac  13740 

tcgcaccacc cgcccgcgcc tgctgggcga ggaggagtac ctaaacaact cgctgctgca  13800 

gccgcagcgc gaaaagaacc tgcctccggc gtttcccaac aacgggatag agagcctagt  13860 

ggacaagatg agtagatgga agacgtatgc gcaggagcac agggatgtgc ccggcccgcg  13920 

cccgcccacc cgtcgtcaaa ggcacgaccg tcagcggggt ctggtgtggg aggacgatga  13980 

ctcggcagac gacagcagcg tcttggattt gggagggagt ggcaacccgt ttgcacacct  14040 

tcgccccagg ctggggagaa tgttttaaaa aaagcatgat gcaaaataaa aaactcacca  14100 

aggccatggc accgagcgtt ggttttcttg tattcccctt agtatgcggc gcgcggcgat  14160 

gtatgaggaa ggtcctcctc cctcctacga gagcgtggtg agcgcggcgc cagtggcggc  14220 

ggcgctgggt tcacccttcg atgctcccct ggacccgccg ttcgtgcctc cgcggtacct  14280 

gcggcctacc ggggggagaa acagcatccg ttactctgag ttggcacccc tattcgacac  14340 

cacccgtgtg taccttgtgg acaacaagtc aacggatgtg gcatccctga actaccagaa  14400 

cgaccacagc aactttctaa ccacggtcat tcaaaacaat gactacagcc cgggggaggc  14460 

aagcacacag accatcaatc ttgacgaccg gtcgcactgg ggcggcgacc tgaaaaccat  14520 

cctgcatacc aacatgccaa atgtgaacga gttcatgttt accaataagt ttaaggcgcg  14580 

ggtgatggtg tcgcgctcgc ttactaagga caaacaggtg gagctgaaat acgagtgggt  14640 

ggagttcacg ctgcccgagg gcaactactc cgagaccatg accatagacc ttatgaacaa  14700 

cgcgatcgtg gagcactact tgaaagtggg caggcagaac ggggttctgg aaagcgacat  14760 

cggggtaaag tttgacaccc gcaacttcag actggggttt gacccagtca ctggtcttgt  14820 

catgcctggg gtatatacaa acgaagcctt ccatccagac atcattttgc tgccaggatg  14880 

cggggtggac ttcacccaca gccgcctgag caacttgttg ggcatccgca agcggcaacc  14940 

cttccaggag ggctttagga tcacctacga tgacctggag ggtggtaaca ttcccgcact  15000 

gttggatgtg gacgcctacc aggcaagctt gaaagatgac accgaacagg gcgggggtgg  15060 

cgcaggcggc ggcaacaaca gtggcagcgg cgcggaagag aactccaacg cggcagctgc  15120 

ggcaatgcag ccggtggagg acatgaacga tcatgccatt cgcggcgaca cctttgccac  15180 

acgggcggag gagaagcgcg ctgaggccga ggcagcggcc gaagctgccg cccccgctgc  15240 

ggaggctgca caacccgagg tcgagaagcc tcagaagaaa ccggtgatta aacccctgac  15300 

agaggacagc aagaaacgca gttacaacct aataagcaat gacagcacct tcacccagta  15360 

ccgcagctgg taccttgcat acaactacgg cgaccctcag gccgggatcc gctcatggac  15420 

cctgctttgc actcctgacg taacctgcgg ctcggagcag gtatactggt cgttgcccga  15480 

catgatgcaa gaccccgtga ccttccgctc cacgcgccag atcagcaact ttccggtggt  15540 

gggcgccgag ctgttgcccg tgcactccaa gagcttctac aacgaccagg ccgtctactc  15600 

ccagctcatc cgccagttta cctctctgac ccacgtgttc aatcgctttc ccgagaacca  15660 

gattttggcg cgcccgccag cccccaccat caccaccgtc agtgaaaacg ttcctgctct  15720 

cacagatcac gggacgctac cgctgcgcaa cagcatcgga ggagtccagc gagtgaccat  15780 

tactgacgcc agacgccgca cctgccccta cgtttacaag gccctgggca tagtctcgcc  15840 

gcgcgtccta tcgagccgca ctttttgagc aagcatgtcc atccttatat cgcccagcaa  15900 

taacacaggc tggggcctgc gcttcccaag caagatgttt ggcggggcca agaagcgctc  15960 

cgaccaacac ccagtgcgcg tgcgcgggca ctaccgcgcg ccctggggcg cgcacaaacg  16020 

cggccgcact gggcgcacca ccgtcgatga cgccatcgac gcggtggtgg aggaggcgcg  16080 

caactacacg cccacgccgc cgccagtgtc caccgtggac gcggccattc agaccgtggt  16140 

gcgcggagcc cggcgctacg ctaaaatgaa gagacggcgg aggcgcgtag cacgtcgcca  16200 

ccgccgccga cccggcactg ccgcccaacg cgcggcggcg gccctgctta accgcgcacg  16260 

tcgcaccggc cgacgggcgg ccatgcgagc cgctcgaagg ctggccgcgg gtattgtcac  16320 

tgtgcccccc aggtccaggc gacgagcggc cgccgcagca gccgcggcca ttagtgctat  16380 

gactcagggt cgcaggggca acgtgtactg ggtgcgcgac tcggttagcg gcctgcgcgt  16440 

gcccgtgcgc acccgccccc cgcgcaacta gattgcaata aaaaactact tagactcgta  16500 

ctgttgtatg tatccagcgg cggcggcgcg catcgaagct atgtccaagc gcaaaatcaa  16560 

agaagagatg ctccaggtca tcgcgccgga gatctatggc cccccgaaga aggaagagca  16620 

ggattacaag ccccgaaagc taaagcgggt caaaaagaaa aagaaagatg atgatgatga  16680 

tgaacttgac gacgaggtgg aactgttgca cgcgaccgcg cccaggcgac gggtacagtg  16740 

gaaaggtcga cgcgtaagac gtgttttgcg acccggcacc accgtagtct ttacgcccgg  16800 

tgagcgctcc acccgcacct acaagcgcgt gtatgatgag gtgtacggcg acgaggacct  16860 

gcttgagcag gccaacgagc gcctcgggga gtttgcctac ggaaagcggc ataaggacat  16920 

gctggcgttg ccgctggacg agggcaaccc aacacctagc ctaaagcccg tgacactgca  16980 

gcaggtgctg cccgcgcttg caccgtccga agaaaagcgc ggcctaaagc gcgagtctgg  17040 

tgacttggca cccaccgtgc agctgatggt acccaagcgt cagcgactgg aagatgtctt  17100 

ggaaaaaatg accgtggagc ctgggctgga gcccgaggtc cgcgtgcggc caatcaagca  17160 

ggtggcaccg ggactgggcg tgcagaccgt ggacgttcag atacccacca ccagtagcac  17220 

tagtattgcc actgccacag agggcatgga gacacaaacg tccccggttg cctcggcggt  17280 

ggcagatgcc gcggtgcagg cggccgctgc ggccgcgtcc aagacctcta cggaggtgca  17340 

aacggacccg tggatgtttc gtgtttcagc cccccggcgt ccgcgccgtt caaggaagta  17400 

cggcgccgcc agcgcgctac tgcccgaata tgccctacat ccttccatcg cgcctacccc  17460 

cggctatcgt ggctacacct accgccccag aagacgagca actacccgac gccgaaccac  17520 

cactggaacc cgccgccgcc gtcgccgtcg ccagcccgtg ctggccccga tttccgtgcg  17580 

cagggtggct cgcgaaggag gcaggaccct ggtgctgcca acagcgcgct accaccccag  17640 

catcgtttaa aagccggtct ttgtggttct tgcagatatg gccctcacct gccgcctccg  17700 

tttcccggtg ccgggattcc gaggaagaat gcaccgtagg aggggcatgg ccggccacgg  17760 

cctgacgggc ggcatgcgtc gtgcgcacca ccggcggcgg cgcgcgtcgc accgtcgcat  17820 

gcgcggcggt atcctgcccc tccttattcc actgatcgcc gcggcgattg gcgccgtgcc  17880 

cggaattgca tccgtggcct tgcaggcgca gagacactga ttaaaaacaa gttacatgtg  17940 

gaaaaatcaa aataaaagtc tggactctca cgctcgcttg gtcctgtaac tattttgtag  18000 

aatggaagac atcaactttg cgtcactggc cccgcgacac ggctcgcgcc cgttcatggg  18060 

aaactggcaa gatatcggca ccagcaatat gagcggtggc gccttcagct ggggctcgct  18120 

gtggagcggc attaaaaatt tcggttccgc cgttaagaac tatggcagca aagcctggaa  18180 

cagcagcaca ggccagatgc tgagggacaa gttgaaagag caaaatttcc aacaaaaggt  18240 

ggtagatggc ctggcctctg gcattagcgg ggtggtggac ctggccaacc aggcagtgca  18300 

aaataagatt aacagtaagc ttgatccccg ccctcccgta gaggagcctc caccggccgt  18360 

ggagacagtg tctccagagg ggcgtggcga aaagcgtccg cgacccgaca gggaagaaac  18420 

tctggtgacg caaatagacg agcctccctc gtacgaggag gcactaaagc aaggcctgcc  18480 

caccacccgt cccatcgcgc ccatggctac cggagtgctg ggccagcaca cacccgtaac  18540 

gctggacctg cctccccccg ccgacaccca gcagaaacct gtgctgccag gcccgtccgc  18600 

cgttgttgta acccgtccta gccgcgcgtc cctgcgccgc gccgccagcg gtccgcgatc  18660 

gttgcggccc gtagccagtg gcaactggca aagcacactg aacagcatcg tgggtttggg  18720 

ggtgcaatcc ctgaagcgcc gacgatgctt ctgatagcta acgtgtcgta tgtgtgtcat  18780 

gtatgcgtcc atgtcgccgc cagaggagct gctgagccgc cgcgcgcccg ctttccaaga  18840 

tggctacccc ttcgatgatg ccgcagtggt cttacatgca catctcgggc caggacgcct  18900 

cggagtacct gagccccggg ctggtgcagt tcgcccgcgc caccgagacg tacttcagcc  18960 

tgaataacaa gtttagaaac cccacggtgg cgcctacgca cgacgtgacc acagaccggt  19020 

ctcagcgttt gacgctgcgg ttcatccccg tggaccgcga ggatactgcg tactcgtaca  19080 

aggcgcggtt caccctagct gtgggtgata accgtgtgct agacatggct tccacgtact  19140 

ttgacatccg cggcgtgctg gacaggggcc ctacttttaa gccctactct ggcactgcct  19200 

acaacgcact ggcccccaag ggtgccccca actcgtgcga gtgggaacaa aatgaaactg  19260 

cacaagtgga tgctcaagaa cttgacgaag aggagaatga agccaatgaa gctcaggcgc  19320 

gagaacagga acaagctaag aaaacccatg tatatgccca ggctccactg tccggaataa  19380 

aaataactaa agaaggtcta caaataggaa ctgccgacgc cacagtagca ggtgccggca  19440 

aagaaatttt cgcagacaaa acttttcaac ctgaaccaca agtaggagaa tctcaatgga  19500 

acgaagcgga tgccacagca gctggtggaa gggttcttaa aaagacaact cccatgaaac  19560 

cctgctatgg ctcatacgct agacccacca attccaacgg cggacagggc gttatggttg  19620 

aacaaaatgg taaattggaa agtcaagtcg aaatgcaatt tttttccaca tccacaaatg  19680 

ccacaaatga agttaacaat atacaaccaa cagttgtatt gtacagcgaa gatgtaaaca  19740 

tggaaactcc agatactcat ctttcttata aacctaaaat gggggataaa aatgccaaag  19800 

tcatgcttgg acaacaagca atgccaaaca gaccaaatta cattgctttt agagacaatt  19860 

ttattggtct catgtattac aacagcacag gtaacatggg tgtccttgct ggtcaggcat  19920 

cgcagttgaa cgctgttgta gatttgcaag acagaaacac agagctgtcc taccagcttt  19980 

tgcttgattc aattggcgac agaacaagat acttttcaat gtggaatcaa gctgttgaca  20040 

gctatgatcc agatgtcaga attattgaga accatggaac tgaggatgag ttgccaaatt  20100 

attgctttcc tcttggtgga attgggatta ctgacacttt tcaagctgtt aaaacaactg  20160 

ctgctaacgg ggaccaaggc aatactacct ggcaaaaaga ttcaacattt gcagaacgca  20220 

atgaaatagg ggtgggaaat aactttgcca tggaaattaa cctgaatgcc aacctatgga  20280 

gaaatttcct ttactccaat attgcgctgt acctgccaga caagctaaaa tacaacccca  20340 

ccaatgtgga aatatctgac aaccccaaca cctacgacta catgaacaag cgagtggtgg  20400 

ctcctgggct tgtagactgc tacattaacc ttggggcgcg ctggtctctg gactacatgg  20460 

acaacgttaa tccctttaac caccaccgca atgcgggcct gcgttaccgc tccatgttgt  20520 

tgggaaacgg ccgctacgtg ccctttcaca ttcaggtgcc ccaaaagttt tttgccatta  20580 

aaaacctcct cctcctgcca ggctcataca catatgaatg gaacttcagg aaggatgtta  20640 

acatggttct gcagagctct ctgggaaacg accttagagt tgacggggct agcattaagt  20700 

ttgacagcat ttgtctttac gccaccttct tccccatggc ccacaacacg gcctccacgc  20760 

tggaagccat gctcagaaat gacaccaacg accagtcctt taatgactac ctttccgccg  20820 

ccaacatgct atatcccata cccgccaacg ccaccaacgt gcccatctcc atcccatcgc  20880 

gcaactgggc agcatttcgc ggttgggcct tcacacgctt gaagacaaag gaaacccctt  20940 

ccctgggatc aggctacgac ccttactaca cctactctgg ctccatacca taccttgacg  21000 

gaaccttcta tcttaatcac acctttaaga aggtggccat tacttttgac tcttctgtta  21060 

gctggccggg caacgaccgc ctgcttactc ccaatgagtt tgagattaag cgctcagttg  21120 

acggggaggg ctataacgta gctcagtgca acatgacaaa ggactggttc ctagtgcaga  21180 

tgttggccaa ctacaatatt ggctaccagg gcttctacat tccagaaagc tacaaagacc  21240 

gcatgtactc gttcttcaga aacttccagc ccatgagccg gcaagtggtg gacgatacta  21300 

aatacaaaga ttatcagcag gttggaatta tccaccagca taacaactca ggcttcgtag  21360 

gctacctcgc tcccaccatg cgcgagggac aagcttaccc cgctaatgtt ccctacccac  21420 

taataggcaa aaccgcggtt gatagtatta cccagaaaaa gtttctttgc gaccgcaccc  21480 

tgtggcgcat ccccttctcc agtaacttta tgtccatggg tgcgctcaca gacctgggcc  21540 

aaaaccttct ctacgcaaac tccgcccacg cgctagacat gacctttgag gtggatccca  21600 

tggacgagcc cacccttctt tatgttttgt ttgaagtctt tgacgtggtc cgtgtgcacc  21660 

agccgcaccg cggcgtcatc gagaccgtgt acctgcgcac gcccttctcg gccggcaacg  21720 

ccacaacata aagaagcaag caacatcaac aacagctgcc gccatgggct ccagtgagca  21780 

ggaactgaaa gccattgtca aagatcttgg ttgtgggcca tattttttgg gcacctatga  21840 

caagcgcttc ccaggctttg tttccccaca caagctcgcc tgcgccatag ttaacacggc  21900 

cggtcgcgag actgggggcg tacactggat ggcctttgcc tggaacccgc gctcaaaaac  21960 

atgctacctc tttgagccct ttggcttttc tgaccaacgt ctcaagcagg tttaccagtt  22020 

tgagtacgag tcactcctgc gccgtagcgc cattgcctct tcccccgacc gctgtataac  22080 

gctggaaaag tccacccaaa gcgtgcaggg gcccaactcg gccgcctgtg gcctattctg  22140 

ctgcatgttt ctccacgcct ttgccaactg gccccaaact cccatggatc acaaccccac  22200 

catgaacctt attaccgggg tacccaactc catgcttaac agtccccagg tacagcccac  22260 

cctgcgccgc aaccaggaac agctctacag cttcctggag cgccactcgc cctacttccg  22320 

cagccacagt gcgcaaatta ggagcgccac ttctttttgt cacttgaaaa acatgtaaaa  22380 

ataatgtact aggagacact ttcaataaag gcaaatgttt ttatttgtac actctcgggt  22440 

gattatttac ccccaccctt gccgtctgcg ccgtttaaaa atcaaagggg ttctgccgcg  22500 

catcgctatg cgccactggc agggacacgt tgcgatactg gtgtttagtg ctccacttaa  22560 

actcaggcac aaccatccgc ggcagctcgg tgaagttttc actccacagg ctgcgcacca  22620 

tcaccaacgc gtttagcagg tcgggcgccg atatcttgaa gtcgcagttg gggcctccgc  22680 

cctgcgcgcg cgagttgcga tacacagggt tacagcactg gaacactatc agcgccgggt  22740 

ggtgcacgct ggccagcacg ctcttgtcgg agatcagatc cgcgtccagg tcctccgcgt  22800 

tgctcagggc gaacggagtc aactttggta gctgccttcc caaaaagggt gcatgcccag  22860 

gctttgagtt gcactcgcac cgtagtggca tcagaaggtg accgtgccca gtctgggcgt  22920 

taggatacag cgcctgcatg aaagccttga tctgcttaaa agccacctga gcctttgcgc  22980 

cttcagagaa gaacatgccg caagacttgc cggaaaactg attggccgga caggccgcgt  23040 

catgcacgca gcaccttgcg tcggtgttgg agatctgcac cacatttcgg ccccaccggt  23100 

tcttcacgat cttggccttg ctagactgct ccttcagcgc gcgctgcccg ttttcgctcg  23160 

tcacatccat ttcaatcacg tgctccttat ttatcataat gctcccgtgt agacacttaa  23220 

gctcgccttc gatctcagcg cagcggtgca gccacaacgc gcagcccgtg ggctcgtggt  23280 

gcttgtaggt tacctctgca aacgactgca ggtacgcctg caggaatcgc cccatcatcg  23340 

tcacaaaggt cttgttgctg gtgaaggtca gctgcaaccc gcggtgctcc tcgtttagcc  23400 

aggtcttgca tacggccgcc agagcttcca cttggtcagg cagtagcttg aagtttgcct  23460 

ttagatcgtt atccacgtgg tacttgtcca tcaacgcgcg cgcagcctcc atgcccttct  23520 

cccacgcaga cacgatcggc aggctcagcg ggtttatcac cgtgctttca ctttccgctt  23580 

cactggactc ttccttttcc tcttgcatcc gcataccccg cgccactggg tcgtcttcat  23640 

tcagccgccg caccgtgcgc ttacctccct tgccgtgctt gattagcacc ggtgggttgc  23700 

tgaaacccac catttgtagc gccacatctt ctctttcttc ctcgctgtcc acgatcacct  23760 

ctggggatgg cgggcgctcg ggcttgggag aggggcgctt ctttttcttt ttggacgcaa  23820 

tggccaaatc cgccgtcgag gtcgatggcc gcgggctggg tgtgcgcggc accagcgcat  23880 

cttgtgacga gtcttcttcg tcctcggact cgagacgccg cctcagccgc ttttttgggg  23940 

gcgcgcgggg aggcggcggc gacggcgacg gggacgagac gtcctccatg gttggtggac  24000 

gtcgcgccgc accgcgtccg cgctcggggg tggtttcgcg ctgctcctct tcccgactgg  24060 

ccatttcctt ctcctatagg cagaaaaaga tcatggagtc agtcgagaag gaggacagcc  24120 

taaccgcccc ctttgagttc gccaccaccg cctccaccga tgccgccaac gcgcctacca  24180 

ccttccccgt cgaggcaccc ccgcttgagg aggaggaagt gattatcgag caggacccag  24240 

gttttgtaag cgaagacgac gaagatcgct cagtaccaac agaggataaa aagcaagacc  24300 

aggacgacgc agaggcaaac gaggaacaag tcgggcgggg ggaccaaagg catggcgact  24360 

acctagatgt gggagacgac gtgctgttga agcatctgca gcgccagtgc gccattatct  24420 

gcgacgcgtt gcaagagcgc agcgatgtgc ccctcgccat agcggatgtc agccttgcct  24480 

acgaacgcca cctgttctca ccgcgcgtac cccccaaacg ccaagaaaac ggcacatgcg  24540 

agcccaaccc gcgcctcaac ttctaccccg tatttgccgt gccagaggtg cttgccacct  24600 

atcacatctt tttccaaaac tgcaagatac ccctatcctg ccgtgccaac cgcagccgag  24660 

cggacaagca gctggccttg cggcagggcg ctgtcatacc tgatatcgcc tcgctcgacg  24720 

aagtgccaaa aatctttgag ggtcttggac gcgacgagaa gcgcgcggca aacgctctgc  24780 

aacaagaaaa cagcgaaaat gaaagtcact gtggagtgct ggtggaactt gagggtgaca  24840 

acgcgcgcct agccgtgctg aaacgcagca tcgaggtcac ccactttgcc tacccggcac  24900 

ttaacctacc ccccaaggtt atgagcacag tcatgagcga gctgatcgtg cgccgtgcac  24960 

gacccctgga gagggatgca aacttgcaag aacaaaccga ggagggccta cccgcagttg  25020 

gcgatgagca gctggcgcgc tggcttgaga cgcgcgagcc tgccgacttg gaggagcgac  25080 

gcaagctaat gatggccgca gtgcttgtta ccgtggagct tgagtgcatg cagcggttct  25140 

ttgctgaccc ggagatgcag cgcaagctag aggaaacgtt gcactacacc tttcgccagg  25200 

gctacgtgcg ccaggcctgc aaaatttcca acgtggagct ctgcaacctg gtctcctacc  25260 

ttggaatttt gcacgaaaac cgccttgggc aaaacgtgct tcattccacg ctcaagggcg  25320 

aggcgcgccg cgactacgtc cgcgactgcg tttacttatt tctgtgctac acctggcaaa  25380 

cggccatggg cgtgtggcag cagtgcctgg aggagcgcaa cctgaaggag ctgcagaagc  25440 

tgctaaagca aaacttgaag gacctatgga cggccttcaa cgagcgctcc gtggccgcgc  25500 

acctggcgga cattatcttc cccgaacgcc tgcttaaaac cctgcaacag ggtctgccag  25560 

acttcaccag tcaaagcatg ttgcaaaact ttaggaactt tatcctagag cgttcaggaa  25620 

ttctgcccgc cacctgctgt gcgcttccta gcgactttgt gcccattaag taccgtgaat  25680 

gccctccgcc gctttggggt cactgctacc ttctgcagct agccaactac cttgcctacc  25740 

actccgacat catggaagac gtgagcggtg acggcctact ggagtgtcac tgtcgctgca  25800 

acctatgcac cccgcaccgc tccctggtct gcaattcaca actgcttagc gaaagtcaaa  25860 

ttatcggtac ctttgagctg cagggtccct cgcctgacga aaagtccgcg gctccggggt  25920 

tgaaactcac tccggggctg tggacgtcgg cttaccttcg caaatttgta cctgaggact  25980 

accacgccca cgagattagg ttctacgaag accaatcccg cccgccaaat gcggagctta  26040 

ccgcctgcgt cattacccag ggccacatcc ttggccaatt gcaagccatt aacaaagccc  26100 

gccaagagtt tctgctacga aagggacggg gggtttactt ggacccccag tccggcgagg  26160 

agctcaaccc aatccccccg ccgccgcagc cctatcagca gccgcgggcc cttgcttccc  26220 

aggatggcac ccaaaaagaa gctgcagctg ccgccgccgc cacccacgga cgaggaggaa  26280 

tactgggaca gtcaggcaga ggaggttttg gacgaggagg aggagatgat ggaagactgg  26340 

gacagcctag acgaggaagc ttccgaggcc gaagaggtgt cagacgaaac accgtcaccc  26400 

tcggtcgcat tcccctcgcc ggcgccccag aaatcggcaa ccgttcccag cattgctaca  26460 

acctccgctc ctcaggcgcc gccggcactg cccgttcgcc gacccaaccg tagatgggac  26520 

accactggaa ccagggccgg taagtctaag cagccgccgc cgttagccca agagcaacaa  26580 

cagcgccaag gctaccgctc gtggcgcgtg cacaagaacg ccatagttgc ttgcttgcaa  26640 

gactgtgggg gcaacatctc cttcgcccgc cgctttcttc tctaccatca cggcgtggcc  26700 

ttcccccgta acatcctgca ttactaccgt catctctaca gcccctactg caccggcggc  26760 

agcggcagca acagcagcgg ccacgcagaa gcaaaggcga ccggatagca agactctgac  26820 

aaagcccaag aaatccacag cggcggcagc agcaggagga ggagcactgc gtctggcgcc  26880 

caacgaaccc gtatcgaccc gcgagcttag aaacaggatt tttcccactc tgtatgctat  26940 

atttcaacag agcaggggcc aagaacaaga gctgaaaata aaaaacaggt ctctgcgctc  27000 

cctcacccgc agctgcctgt atcacaaaag cgaagatcag cttcggcgca cgctggaaga  27060 

cgcggaggct ctcttcagca aatactgcgc gctgactctt aaggactagt ttcgcgccct  27120 

ttctcaaatt taagcgcgaa aactacgtca tctccagcgg ccacacccgg cgccagcacc  27180 

tgtcgtcagc gccattatga gcaaggaaat tcccacgccc tacatgtgga gttaccagcc  27240 

acaaatggga cttgcggctg gagctgccca agactactca acccgaataa actacatgag  27300 

cgcgggaccc cacatgatat cccgggtcaa cggaatccgc gcccaccgaa accgaattct  27360 

cctcgaacag gcggctatta ccaccacacc tcgtaataac cttaatcccc gtagttggcc  27420 

cgctgccctg gtgtaccagg aaagtcccgc tcccaccact gtggtacttc ccagagacgc  27480 

ccaggccgaa gttcagatga ctaactcagg ggcgcagctt gcgggcggct ttcgtcacag  27540 

ggtgcggtcg cccgggcagg gtataactca cctgaaaatc agagggcgag gtattcagct  27600 

caacgacgag tcggtgagct cctctcttgg tctccgtccg gacgggacat ttcagatcgg  27660 

cggcgctggc cgctcttcat ttacgccccg tcaggcgatc ctaactctgc agacctcgtc  27720 

ctcggagccg cgctccggag gcattggaac tctacaattt attgaggagt tcgtgccttc  27780 

ggtttacttc aacccctttt ctggacctcc cggccactac ccggaccagt ttattcccaa  27840 

ctttgacgcg gtaaaagact cggcggacgg ctacgactga atgaccagtg gagaggcaga  27900 

gcaactgcgc ctgacacacc tcgaccactg ccgccgccac aagtgctttg cccgcggctc  27960 

cggtgagttt tgttactttg aattgcccga agagcatatc gagggcccgg cgcacggcgt  28020 

ccggctcacc acccaggtag agcttacacg tagcctgatt cgggagttta ccaagcgccc  28080 

cctgctagtg gagcgggagc ggggtccctg tgttctgacc gtggtttgca actgtcctaa  28140 

ccctggatta catcaagatc tttgttgtca tctctgtgct gagtataata aatacagaaa  28200 

ttagaatcta ctggggctcc tgtcgccatc ctgtgaacgc caccgttttt acccacccaa  28260 

agcagaccaa agcaaacctc acctccggtt tgcacaagcg ggccaataag taccttacct  28320 

ggtactttaa cggctcttca tttgtaattt acaacagttt ccagcgagac gaagtaagtt  28380 

tgccacacaa ccttctcggc ttcaactaca ccgtcaagaa aaacaccacc accaccctcc  28440 

tcacctgccg ggaacgtacg agtgcgtcac cggttgctgc gcccacacct acagcctgag  28500 

cgtaaccaga cattactccc attttcccaa aacaggaggt gagctcaact cccggaactc  28560 

aggtcaaaaa agcattttgc ggggtgctgg gattttttaa ttaagtatat gagcaattca  28620 

agtaactcta caagcttgtc taatttttct ggaattgggg tcggggttat ccttactctt  28680 

gtaattctgt ttattcttat actagcactt ctgtgcctta gggttgccgc ctgctgcacg  28740 

cacgtttgta cctattgtca gctttttaaa cgctgggggc gacatccaag atgaggtaca  28800 

tgattttagg cttgctcgcc cttgcggcag tctgcagcgc tgccaaaaag gttgagttta  28860 

aggaaccagc ttgcaatgtt acatttaaat cagaagctaa tgaatgcact actcttataa  28920 

aatgcaccac agaacatgaa aagcttatta ttcgccacaa agacaaaatt ggcaagtatg  28980 

ctgtatatgc tatttggcag ccaggtgaca ctaacgacta taatgtcaca gtcttccaag  29040 

gtgaaaatcg taaaactttt atgtataaat ttccatttta tgaaatgtgc gatattacca  29100 

tgtacatgag caaacagtac aagttgtggc ccccacaaaa gtgtttagag aacactggca  29160 

ccttttgttc caccgctctg cttattacag cgcttgcttt ggtatgtacc ttactttatc  29220 

tcaaatacaa aagcagacgc agttttattg atgaaaagaa aatgccttga ttttccgctt  29280 

gcttgtattc ccctggacaa tttactctat gtgggatatg cgccaggcgg gaaagattat  29340 

acccacaacc ttcaaatcaa actttcctgg acgttagcgc ctgacttctg ccagcgcctg  29400 

cactgcaaat ttgatcaaac ccagcttcag cttgcctgct ccagagatga ccggctcaac  29460 

catcgcgccc acaacggact atcgcaacac cactgctacc ggactaaaat ctgccctaaa  29520 

tttaccccaa gttcatgcct ttgtcaatga ctgggcgagc ttgggcatgt ggtggttttc  29580 

catagcgctt atgtttgttt gccttattat tatgtggctt atttgttgcc taaagcgcag  29640 

acgcgccaga ccccccatct ataggcctat cattgtgctc aacccacaca atgaaaaaat  29700 

tcatagattg gacggtctca aaccatgttc tcttctttta cagtatgatt aaatgagaca  29760 

tgattcctcg agtccttata ttattgaccc ttgttgcgct tttctgtgcg tgctctacat  29820 

tggctgcggt cgctcacatc gaagtagatt gcatcccacc tttcacagtt tacctgcttt  29880 

acggatttgt cacccttatc ctcatctgca gcctcgtcac tgtagtcatc gccttcattc  29940 

agttcattga ctggatttgt gtgcgcattg cgtaccttag gcaccatccg caatacagag  30000 

acaggactat agctgatctt ctcagaattc tttaattatg aaacggattg tcacttttgt  30060 

tttgctgatt ttctgcgccc tacctgtgct ttgctcccaa acctcagcgc ctcccaaaag  30120 

acatatttcc tgcagattca ctcaaatatg gaacattccc agctgctaca acaaacagag  30180 

cgatttgtca gaagcctggt tatacgccat catctctgtc atggtttttt gcagtaccat  30240 

ttttgcccta gccatatacc cataccttga cattggttgg aatgccatag atgccatgaa  30300 

ccaccctact ttcccagcgc ccaatgtcat accactgcaa caggttattg ccccaatcaa  30360 

tcagcctcgc cccccttctc ccacccccac tgagattagc tactttaatt tgacaggtgg  30420 

agatgactga atctctagat ctagaattgg atggaattaa caccgaacag cgcctactag  30480 

aaaggcgcaa ggcggcgtcc gagcgagaac gcctaaaaca agaagttgaa gacatggtta  30540 

acctgcacca gtgtaaaaga ggtatctttt gtgtggtcaa gcaggccaaa cttacctacg  30600 

aaaaaaccac taccggcaac cgccttagct acaagctacc cacccagcgc caaaaactgg  30660 

tgcttatggt gggagaaaaa cctatcaccg tcacccagca ctcggcagaa acagaaggct  30720 

gcctgcactt cccctatcag ggtccagagg acctctgcac tcttattaaa accatgtgtg  30780 

gcattagaga tcttattcca ttcaactaac aataaacaca caataaatta cttacttaaa  30840 

atcagtcagc aaatctttgt ccagcttatt cagcatcacc tcctttccct cctcccaact  30900 

ctggtatttc agcagccttt tagctgcgaa ctttctccaa agtctaaatg ggatgtcaaa  30960 

ttcctcatgt tcttgtccct ccgcacccac tatcttcata ttgttgcaga tgaaacgcgc  31020 

cagaccgtct gaagacacct tcaaccctgt gtacccatat gacacggaaa ccggccctcc  31080 

aactgtgcct ttccttaccc ctccctttgt gtcgccaaat gggttccaag aaagtccccc  31140 

cggagtgctt tctttgcgtc tttcagaacc tttggttacc tcacacggca tgcttgcgct  31200 

aaaaatgggc agcggcctgt ccctggatca ggcaggcaac cttacatcaa atacaatcac  31260 

tgtttctcaa ccgctaaaaa aaacaaagtc caatataact ttggaaacat ccgcgcccct  31320 

tacagtcagc tcaggcgccc taaccatggc cacaacttcg cctttggtgg tctctgacaa  31380 

cactcttacc atgcaatcac aagcaccgct aaccgtgcaa gactcaaaac ttagcattgc  31440 

taccaaagag ccacttacag tgttagatgg aaaactggcc ctgcagacat cagcccccct  31500 

ctctgccact gataacaacg ccctcactat cactgcctca cctcctctta ctactgcaaa  31560 

tggtagtctg gctgttacca tggaaaaccc actttacaac aacaatggaa aacttgggct  31620 

caaaattggc ggtcctttgc aagtggccac cgactcacat gcactaacac taggtactgg  31680 

tcagggggtt gcagttcata acaatttgct acatacaaaa gttacaggcg caatagggtt  31740 

tgatacatct ggcaacatgg aacttaaaac tggagatggc ctctatgtgg atagcgccgg  31800 

tcctaaccaa aaactacata ttaatctaaa taccacaaaa ggccttgctt ttgacaacac  31860 

cgcaataaca attaacgctg gaaaagggtt ggaatttgaa acagactcct caaacggaaa  31920 

tcccataaaa acaaaaattg gatcaggcat acaatataat accaatggag ctatggttgc  31980 

aaaacttgga acaggcctca gttttgacag ctccggagcc ataacaatgg gcagcataaa  32040 

caatgacaga cttactcttt ggacaacacc agacccatcc ccaaattgca gaattgcttc  32100 

agataaagac tgcaagctaa ctctggcgct aacaaaatgt ggcagtcaaa ttttgggcac  32160 

tgtttcagct ttggcagtat caggtaatat ggcctccatc aatggaactc taagcagtgt  32220 

aaacttggtt cttagatttg atgacaacgg agtgcttatg tcaaattcat cactggacaa  32280 

acagtattgg aactttagaa acggggactc cactaacggt caaccataca cttatgctgt  32340 

tgggtttatg ccaaacctaa aagcttaccc aaaaactcaa agtaaaactg caaaaagtaa  32400 

tattgttagc caggtgtatc ttaatggtga caagtctaaa ccattgcatt ttactattac  32460 

gctaaatgga acagatgaaa ccaaccaagt aagcaaatac tcaatatcat tcagttggtc  32520 

ctggaacagt ggacaataca ctaatgacaa atttgccacc aattcctata ccttctccta  32580 

cattgcccag gaataaagaa tcgtgaacct gttgcatgtt atgtttcaac gtgtttattt  32640 

ttcaattgca gaaaatttca agtcattttt cattcagtag tatagcccca ccaccacata  32700 

gcttatacta atcaccgtac cttaatcaaa ctcacagaac cctagtattc aacctgccac  32760 

ctccctccca acacacagag tacacagtcc tttctccccg gctggcctta aacagcatca  32820 

tatcatgggt aacagacata ttcttaggtg ttatattcca cacggtctcc tgtcgagcca  32880 

aacgctcatc agtgatgtta ataaactccc cgggcagctc gcttaagttc atgtcgctgt  32940 

ccagctgctg agccacaggc tgctgtccaa cttgcggttg ctcaacgggc ggcgaaggag  33000 

aagtccacgc ctacatgggg gtagagtcat aatcgtgcat caggataggg cggtggtgct  33060 

gcagcagcgc gcgaataaac tgctgccgcc gccgctccgt cctgcaggaa tacaacatgg  33120 

cagtggtctc ctcagcgatg attcgcaccg cccgcagcat aaggcgcctt gtcctccggg  33180 

cacagcagcg caccctgatc tcacttaagt cagcacagta actgcagcac agtaccacaa  33240 

tattgtttaa aatcccacag tgcaaggcgc tgtatccaaa gctcatggcg gggaccacag  33300 

aacccacgtg gccatcatac cacaagcgca ggtagattaa gtggcgaccc ctcataaaca  33360 

cgctggacat aaacattacc tcttttggca tgttgtaatt caccacctcc cggtaccata  33420 

taaacctctg attaaacatg gcgccatcca ccaccatcct aaaccagctg gccaaaacct  33480 

gcccgccggc tatgcactgc agggaaccgg gactggaaca atgacagtgg agagcccagg  33540 

actcgtaacc atggatcatc atgctcgtca tgatatcaat gttggcacaa cacaggcaca  33600 

cgtgcataca cttcctcagg attacaagct cctcccgcgt cagaaccata tcccagggaa  33660 

caacccattc ctgaatcagc gtaaatccca cactgcaggg aagacctcgc acgtaactca  33720 

cgttgtgcat tgtcaaagtg ttacattcgg gcagcagcgg atgatcctcc agtatggtag  33780 

cgcgtgtctc tgtctcaaaa ggaggtaggc gatccctact gtacggagtg cgccgagaca  33840 

accgagatcg tgttggtcgt agtgtcatgc caaatggaac gccggacgta gtcatatttc  33900 

ctgaagcaaa accaggtgcg ggcgtgacaa acagatctgc gtctccggtc tcgtcgctta  33960 

gctcgctctg tgtagtagtt gtagtatatc cactctctca aagcatccag gcgccccctg  34020 

gcttcgggtt ctatgtaaac tccttcatgc gccgctgccc tgataacatc caccaccgca  34080 

gaataagcca cacccagcca acctacacat tcgttctgcg agtcacacac gggaggagcg  34140 

ggaagagctg gaagaaccat gttttttttt tttattccaa aagattatcc aaaacctcaa  34200 

aatgaagatc tattaagtga acgcgctccc ctccggtggc gtggtcaaac tctacagcca  34260 

aagaacagat aatggcattt gtaagatgtt gcacaatggc ttccaaaagg caaactgccc  34320 

tcacgtccaa gtggacgtaa aggctaaacc cttcagggtg aatctcctct ataaacattc  34380 

cagcaccttc aaccatgccc aaataatttt catctcgcca ccttatcaat atgtctctaa  34440 

gcaaatcccg aatattaagt ccggccattg taaaaatctg ctccagagcg ccctccacct  34500 

tcagcctcaa gcagcgaatc atgattgcaa aaattcaggt tcctcacaga cctgtataag  34560 

attcaaaagc ggaacattaa caaaaatacc gcgatcccgt aggtcccttc gcagggccag  34620 

ctgaacataa tcgtgcaggt ctgcacggac cagcgcggcc acttccccgc caggaaccat  34680 

gacaaaagaa cccacactga ttatgacacg catactcgga gctatgctaa ccagcgtagc  34740 

cccgatgtaa gcttgttgca tgggcggcga tataaaatgc aaggtactgc tcaaaaaatc  34800 

aggcaaagcc tcgcgcaaaa aagcaagcac atcgtagtca tgctcatgca gataaaggca  34860 

ggtaagttcc ggaaccacca cagaaaaaga caccattttt ctctcaaaca tgtctgcggg  34920 

ttcctgcata aacacaaaat aaaataacaa aaaaaaaaaa acatttaaac attagaagcc  34980 

tgtnttacaa caggaaaaac aacccttata agcataagac ggactacggc catgccggcg  35040 

tgaccgtaaa aaaactggtc accgtgatta aaaagcacca ccgacagttc ctcggtcatg  35100 

tccggagtca taatgtaaga ctcggtaaac acatcaggtt ggttaacatc ggtcagtgct  35160 

aaaaagcgac cgaaatagcc cgggggaata catacccgca ggcgtagaga caacattaca  35220 

gcccccatag gaggtataac aaaattaata ggagagaaaa acacataaac acctgaaaaa  35280 

ccctcctgcc taggcaaaat agcaccctcc cgctccagaa caacatacag cgcttccaca  35340 

gcggcagcca taacagtcag ccttaccagt aaaaaaacct attaaaaaac accactcgac  35400 

acggcaccag ctcaatcagt cacagtgtaa aaagggccaa gtacagagcg agtatatata  35460 

ggactaaaaa atgacgtaac ggttaaagtc cacaaaaacc acccagaaaa ccgcacgcga  35520 

acctacgccc agaaacgaaa gccaaaaaac ccacaacttc ctcaaatctt cacttccgtt  35580 

ttcccacgat acgtcacttc ccattttaaa aaaaaactac aattcccaat acatgcaagt  35640 

tactccgccc taaaacctac gtcacccgcc ccgttcccac gccccgcgcc acgtcacaaa  35700 

ctccaccccc tcattatcat attggcttca atccaaaata aggtatatta ttgatgatg   35759 

 
           
             10  
             19  
             DNA  
             Artificial Sequence  
             
               oligonucleotide  
             
           
            10 

actcgagatg tatagatct                                                  19 

 
           
             11  
             20  
             DNA  
             Artificial Sequence  
             
               oligonucleotide  
             
           
            11 

ctagatctat acatctcgag                                                 20 

 
           
             12  
             26  
             DNA  
             Artificial Sequence  
             
               PCR primer  
             
           
            12 

gcacagatct ttgcttcagg aatatg                                          26 

 
           
             13  
             29  
             DNA  
             Artificial Sequence  
             
               PCR primer  
             
           
            13 

gagaactcga ggcctacatg ggggtagag                                       29 

 
           
             14  
             26  
             DNA  
             Artificial Sequence  
             
               PCR primer  
             
           
            14 

cgagacggcc gacgcagatc tgtttg                                          26 

 
           
             15  
             25  
             DNA  
             Artificial Sequence  
             
               PCR primer  
             
           
            15 

gaagtcccgg gctacatggg ggtag                                           25