Abstract:
A novel cotton variety, designated as FM 991B2R, is disclosed. The invention relates to seeds, plants, plant cells, plant tissue, harvested products and cotton lint as well as to hybrid cotton plants and seeds obtained by crossing plants of variety FM 991B2R with other plants. The invention also relates to plants and varieties produced by the method of essential derivation from plants of FM 99IB2R and to plants of FM 991B2R reproduced by vegetative methods, including but not limited to tissue culture of regenerable cells or tissue from FM 991B2R.

Description:
CROSS-REFERENCE TO RELATED APPLICATIONS 
     This application claims the benefit of U.S. Provisional Application No. 60/649,658, filed Feb. 3, 2005, the disclosure of which is herein incorporated by reference in its entirety. 
    
    
     BACKGROUND OF THE INVENTION 
     (i) Field of the Invention 
     This invention relates to the field of plant breeding. More particularly, the invention encompasses a variety of cotton designated as FM 991B2R, its essentially derived varieties and the hybrid varieties obtained by crossing FM 991B2R as a parent line with plants of other varieties or parent lines. 
     (ii) Description of the Related Art 
     Cotton is an important, fiber producing crop. In particular, due to the importance of cotton to the textile industry, cotton breeders are increasingly seeking to obtain healthy, good yielding crops of an excellent quality. Cotton is commonly reproduced by self-pollination and fertilization. This type of sexual reproduction facilitates the preservation of plant and variety characteristics during breeding and seed production. The preservation of these characteristics are often important to plant breeders for producing cotton plants having desired traits. Other methods of producing cotton plants having desired traits are also used and include methods such as genetic transformation via  Agrobacterium  infection or direct transfer by microparticle bombardment. 
     Due to the environment, the complexity of the structure of genes and location of a gene in the genome, among other factors, it is difficult to predict the phenotypic expression of a particular genotype. In addition, a plant breeder may only apply his skills on the phenotype and not, or in a very limited way, on the level of the genotype. Because of this phenomenon, a plant breeder cannot breed the same variety twice using the same parents and the same methodology, thus a newly bred variety is considered to be an unexpected result of the breeding process. In particular, each variety will typically contain a unique combination of known or novel characteristics. 
     However, by carefully choosing the breeding parents, the breeding and selection methods, the testing layout and testing locations, the breeder may breed a particular variety type. In addition, the new variety may be tested in special comparative trials with other existing varieties in order to determine whether the new variety meets the required expectations. 
     SUMMARY OF THE INVENTION 
     The present invention relates to seeds, plants, plant cells, parts of plants, cotton lint or fiber, and cotton textiles of the cotton variety designated as FM 991B2R, as well as to hybrid cotton plants and seeds obtained by (repeatedly) crossing plants of FM 991B2R with other cotton plants. The invention encompasses plants and plant varieties produced by the method of essential derivation from plants of FM 991B2R and to plants of FM 991B2R reproduced by vegetative methods, including but not limited to, regeneration of embryogenic cells or tissue of FM 991B2R. 
    
    
     DETAILED DESCRIPTION OF PREFERRED EMBODIMENTS 
     As used herein, “cotton” refers to plants, seeds or plant cells of the genus  Gossypium , preferably the species  Gossypium hirsutum.    
     As used herein, “lint” refers to the mass of soft fibers surrounding the seeds of unginned cotton. 
     As used herein, “phenotypic characteristics” refers to the observable, measurable or otherwise determinable physical or biochemical characteristics of an organism, as determined by both genetic makeup and environmental influences (e.g., length, color, size, weight, biochemical composition, protein concentration, etc.). 
     As used herein, “fiber” refers to the natural filament of cotton, capable of being spun into yarn, as is typically separated from unginned cotton seed. 
     The invention has been obtained by a general breeding process comprising the steps outlined below. (For reference, see Chapter 11, “Breeding Self-Pollinated Crops by Hybridization and Pedigree Selection,” in F. N. Briggs and P. F Knowles (1967)). 
     Parent plants, which have been selected for good agronomic and fiber quality traits are manually crossed in different combinations. The resulting F1 (Filial generation 1) plants are self fertilized and the resulting F2 generation plants, which show a large variability on account of optimal gene segregation, are planted in a selection field. 
     These F2 plants are observed during the growing season for health, growth vigor, plant type, plant structure, leaf type, stand ability, flowering, maturity, seed yield, boll type, boll distribution, boll size, fiber yield and fiber quality. Plants are then selected. The selected plants are harvested, the bolls are analyzed for fiber characteristics and the seeds are cleaned and stored. This procedure is repeated in the following growing seasons, whereby the selection and testing units increase from individual plants in the F2, to multiple plants containing ‘lines’ (descending from one mother plant) in the F5 and the number of units decrease from approximately 2500 plants in the F2 to 20 lines in the F5 by selecting about 10-20% of the units in each selection cycle. 
     The increased size of the units, whereby more seed per unit is available, allows the selection and testing in replicated trials on more than one location with a different environment and a more extensive and accurate analyzing of the fiber quality. 
     The lines or candidate varieties become genotypically more homozygous and phenotypically more homogeneous by selecting similar plant types within a line and by discarding the so called off-types from the very variable F2 generation on to the final F7 or F8 generation. 
     Depending on the intermediate results the plant breeder may decide to vary the procedure as described above such as by accelerating the process by testing a particular line earlier or retesting a line another year. He may also select plants for further crossing with existing parent plants or with other plants resulting from the current selection procedure. 
     By the method of recurrent backcrossing, as described by Briggs and Knowles, supra, in Chapter 13, “The Backcross Method of Breeding,” the breeder may introduce a specific trait or traits into an existing, valuable line or variety, while otherwise preserving the unique combination of characteristics of this line or variety. In this crossing method, the valuable parent is recurrently used to cross it at least for three times with each resulting backcross F1, followed by selection of the recurrent parent plant type, until the phenotype of the resulting F1 is similar or almost identical to the phenotype of the recurrent parent with the addition of the expression of the desired trait or traits. 
     This method of recurrent backcrossing eventually results in an essentially derived variety, which is predominantly derived from the recurrent parent or initial variety. This method can therefore also be used to get as close as possible to the genetic composition of an existing successful variety. Thus, compared to the recurrent parent the essentially derived variety retains a distinctive trait, which can be any phenotypic trait, with the intention to profit from the qualities of that successful initial variety. 
     Depending on the number of backcrosses and the efficacy of the selection of the recurrent parent plant type and genotype, which can be supported by the use of molecular markers as described by P. Stam (2003), the genetic conformity with the initial variety of the resulting essentially derived variety may vary between 90% and 100%. 
     Except via recurrent backcrossing, as described above, such essentially derived variety may also be obtained by the selection from an initial variety of an induced or natural occurring mutant plant, an occurring variant (off-type) plant, a somaclonal variant plant or by genetic transformation of regenerable plant tissue or embryogenic cell cultures of the said initial variety by methods well known to those skilled in the art, such as, for example, by Agrobacterium-mediated transformation as described by Sakhanokho et al. (2004), Reynaerts et al. (2000), Umbeck et al. (1988) and others. Examples of transgenic Events transformed in this way are “LLCotton25,” USDA-APHIS petition 02-042-01p, “Cot 102,” USDA-APHIS petition 03-155-01p and “281-24-236,” USDA-APHIS petition 03-036-01p, combined with “3006-210-23,” USDA-APHIS petition 03-036-02p. Information regarding these and other transgenic events referred to herein may be found at the U.S. Department of Agriculture&#39;s (USDA) Animal and Plant Health Inspection Service (APHIS) website. An “Event” is defined as a (artificial) genetic locus that, as a result of genetic engineering, carries a foreign DNA comprising at least one copy of the gene(s) of interest. 
     The plants selected or transformed retain the unique combination of characteristics of 991B2R, except for the different expression of one, two, three, four or five characteristics changed by the selection of the mutant or variant plant or the one, two, three, four or five different characteristics added, e.g., by genetic transformation. 
     The product of essential derivation is an essentially derived variety, which is, except for the one, two, three, four or five distinctive characteristics, which characteristics are different as the result of the act of derivation, characterized by the same combination of expression of the characteristics in its phenotype as in the phenotype of the initial variety, which same combination of expression results from the genotype that is nearly identical or almost identical or similar to the genotype of the initial variety. Plants of the essentially derived variety can be used to repeat the process of essential derivation. The result of this process is also a variety essentially derived from said initial variety. 
     FM 991B2R has been obtained by introducing the Events “MON531” (USDA-APHIS petition 94-308-01p, the disclosure of which is herein incorporated by reference in its entirety) in combination with “MON15985” (USDA-APHIS petition 00-342-01p, the disclosure of which is herein incorporated by reference in its entirety) and “MON1445” (USDA-APHIS petition 95-045-01p, see also Publication No. US 2004/0148666 and U.S. Pat. No. 6,740,488, the disclosure of each of which is herein incorporated by reference in their entireties), via a cross between a donor plant containing these Events and the cotton variety “FM 991” (Australian Plant Breeders Right Application 1996/088, filed Apr. 19, 1996, issued Feb. 28, 1997), followed by three backcrosses of the F1 plants resulting from these crosses, that express the characteristics of FM 991 combined with the Events as described above, with plants of FM 991. The resulting variety FM 991B2R is similar to the existing variety FM 991, but differs by its resistance to the insect pests Cotton Bollworm, Cotton Leafworm, Fall Armyworm, Pink Bollworm and Tobacco Budworm, as a result of the surprising expression of the Events MON531 and MON15985 in combination with the remainder of the characteristics of FM 991 and the resistance to the herbicide glyfosate as a result of the surprising expression of the Event MON1445 in combination with the remainder of the characteristics of FM 991. 
     Provided herein as embodiments of the invention are seeds, plants, plant cells and parts of plants of the cotton variety FM 991B2R. Representative seeds of this variety have been deposited with the NCIMB Ltd, (Ferguson Building, Craibstone Estate, Bucksburn, Aberdeen, AB21 9YA. Scotland, UK), under the Budapest treaty on Jan. 3, 2007, under NCIMB Accession No. 41458. Plants produced by growing such seeds are prodded herein as embodiments of the invention. Also provided herein are pollen or ovules of these plants, as well as a cell or tissue culture of regenerable cells from such plants. In another embodiment, the invention provides for a cotton plant regenerated from such cell or tissue culture, wherein the regenerated plant has the morphological and physiological characteristics of cotton cultivar FM991B2R, as described the FM991B2R variety characteristics in Table 1, when grown in the same environmental conditions. In yet another embodiment, the invention provides methods of testing for a plant having the morphological and physiological characteristics of cotton cultivar FM991B2R. In one embodiment, the testing for a plant having the morphological and physiological characteristics of cotton cultivar FM991B2R is carried out in the same field, under the same conditions and in the presence of plants of FM991B2R, e.g; plants grown from the seed deposited under NCIMB Accession number 41458. In another embodiment, the characteristics to be tested for are those listed in Table 1. 
     Another embodiment of the invention provides for a method of introducing a desired trait into cotton cultivar FM991B2R comprising: (a) crossing the FM991B2R plants, grown from seed deposited under NCIMB Accession No. 41458, with plants of another cotton line that comprise a desired trait to produce F1 progeny plants, wherein the desired trait is selected from the group consisting of male sterility, herbicide resistance, insect resistance and resistance to bacterial, fungal or viral disease; (b) selecting F1 progeny plants that have the desired trait to produce selected F1 progeny plants; (c) crossing the selected F1 progeny plants with the FM991B2R plants to produce first backcross progeny plants; (d) selecting for first backcross progeny plants that have the desired trait and the physiological and morphological characteristics of cotton cultivar FM991B2R as described in the FM991B2R variety characteristics in Table 1, when grown in the same environmental conditions, to produce selected first backcross progeny plants; and (e) repeating steps (c) and (d) two or more times in succession to produce selected third or higher backcross progeny plants that comprise the desired trait and all of the physiological and morphological characteristics of cotton cultivar FM991B2R as described in the FM991B2R variety characteristics in Table 1, when grown in the same environmental conditions. Also included herein is a plant produced by this method, wherein such plant has the desired trait and all of the physiological and morphological characteristics, when grown in the same environmental conditions, of cotton cultivar FM991B2R, representative seed of which having been deposited under NCIMB Accession No. 41458. 
     Also included herein is a method of producing cotton seed, comprising the steps of using the plant grown from seed of cotton variety FM 991B2R, of which a representative seed sample has been deposited under NCIMB Accession No. 41458, as recurrent parent in crosses with other cotton plants different from FM 991B2R, and harvesting the resultant cotton seed. 
     Another embodiment of this invention relates to seeds, plants, plant cells and parts of plants of cotton varieties that are essentially derived from FM 991B2R, being essentially the same as this invention by expressing the unique combination of characteristics of FM 991B2R, including the insect resistance and herbicide resistance of FM 991B2R, except for one, two, three, four or five characteristics being different from the characteristics of FM 991B2R as a result of the act of derivation. 
     Another embodiment of this invention is the reproduction of plants of FM 991B2R by the method of tissue culture from any regenerable plant tissue obtained from plants of this invention. Plants reproduced by this method express the specific combination of characteristics of this invention and fall within its scope. During one of the steps of the reproduction process via tissue culture somaclonal, variant plants may occur, which plants can be selected as being distinct from this invention, but still fall within the scope of this invention as being essentially derived from this invention. 
     Another embodiment of this invention is the production of a hybrid variety comprising (repeatedly) crossing plants of FM 991B2R with plants of a different variety or varieties or with plants of a non-released line or lines. In practice, three different types of hybrid varieties may be produced (see e.g., Chapter 18, “Hybrid Varieties” in Briggs and Knowles, supra): 
     The “single cross hybrid” produced by two different lines, the “three way hybrid,” produced by three different lines such that first the single hybrid is produced by using two out of the three lines followed by crossing this single hybrid with the third line and the “four way hybrid” produced by four different lines such that first two single hybrids are produced using the lines two by two, followed by crossing the two single hybrids so produced. 
     Each single, three way or four way hybrid variety so produced and using FM 991B2R as one of the parent lines contains an essential contribution of FM 991B2R to the resulting hybrid variety and falls within the scope of this invention. 
     Cotton lint or fiber produced by the plants of this invention and by plants reproduced from this invention and by plants essentially derived from this invention have the unique combination of the quality characteristics of FM 991B2R and fall within the scope of this invention. The final textile produced form the unique fiber of FM 991B2R also falls within the scope of this invention. 
     It will be clear that the invention may be practiced otherwise than as particularly described in the foregoing description and examples. Numerous modifications and variations of the present invention are possible in light of the above teachings and, therefore, are within the scope of the appended claims. 
     The entire disclosure of each document cited (including patents, patent applications, journal articles, abstracts, laboratory manuals, books or other disclosures) in the Background of the Invention, Detailed Description and Examples is hereby incorporated herein by reference. 
     EXAMPLE 1 
     Seeds of the variety FM 991B2R (a representative sample of such seeds deposited under NCIMB Accession No. 41458) were planted, together with seeds of cotton variety DP555BGR as a reference variety, in field trials at two locations, as mentioned hereunder. 
     The results as shown in Table 1 and 2 were obtained from a pooled analysis of the data from these two trials: 
     1. BCSI Research Station, Leland Miss., 2004. Conditions: field grown irrigated trial with conventional management. Trial design: 5 entry trial in a row and column design with six replicates and 14 m plots. Measurements on 10 plants from each plot. 
     2. Australian Cotton Research Institute, Narrabri, NSW, 2003/04 summer. Conditions: field grown irrigated trial with conventional management. Trial design: 24 entry trial in a row and column design with six replicates and two rows×14 m plots. Measurements on 10 plants from each plot. 
     Analysis of variance procedures were used to obtain least significant difference statistics at the 1% level. 
     The description as mentioned in Table 1 and 2 reflect the average expression of the characteristics of FM 991B2R on these locations in 2003/2004. This expression can be different on other locations and/or in other years. The sample that will be deposited represents the variety and this sample can be analyzed for the expression of its phenotypic characteristics at any time and at any location. 
     
       
         
               
             
               
               
               
             
               
               
               
               
             
               
               
               
               
             
           
               
                 TABLE 1 
               
             
             
               
                   
               
               
                 Descriptive Characteristics of FM 991B2R 
               
             
          
           
               
                 Description of 
                 possible 
                 Variety 
               
             
          
           
               
                 Characteristic 
                 expression/note 
                 FM 991B2R 
                 DP555BG/RR 
               
               
                   
               
             
          
           
               
                 General Plant 
                   
                   
                   
               
               
                 Type 
               
               
                 Plant Habit 
                 spreading, inter- 
                 inter- 
                 inter- 
               
               
                   
                 mediate, compact 
                 mediate 
                 mediate 
               
               
                 Foliage 
                 sparse, inter- 
                 inter- 
                 inter- 
               
               
                   
                 mediate, dense 
                 mediate 
                 mediate 
               
               
                 Stem Lodging 
                 lodging, inter- 
                 erect 
                 erect 
               
               
                   
                 mediate, erect 
               
               
                 Fruiting Branch 
                 clustered, short, 
                 short 
                 normal 
               
               
                   
                 normal 
               
               
                 Growth 
                 determinate, 
                 indeter- 
                 indeter- 
               
               
                   
                 intermediate, 
                 minate 
                 minate 
               
               
                   
                 indeterminate 
               
               
                 Leaf color 
                 greenish yellow, 
                 medium 
                 medium 
               
               
                   
                 light green, 
                 green 
                 green 
               
               
                   
                 medium green, 
               
               
                   
                 dark green 
               
               
                 Boll Shape 
                 Length &lt; Width, 
                 L &gt; W 
                 L &gt; W 
               
               
                   
                 L = W, L &gt; W 
               
               
                 Boll Breadth 
                 broadest at base, 
                 broadest 
                 broadest 
               
               
                   
                 broadest at middle 
                 middle 
                 middle 
               
               
                 Maturity 
                 date of 50% open 
                 October 2 
                 October 4 
               
               
                   
                 bolls 
               
               
                 Plant 
               
               
                 cm. to first 
                 from cotyledonary 
                 17.3 
                 23.3 
               
               
                 Fruiting Branch 
                 node 
               
               
                 No. of nodes to 
                 excluding 
                 6.5 
                 7.3 
               
               
                 1st Fruiting 
                 cotyledonary node 
               
               
                 Branch 
               
               
                 Mature Plant 
                 cotyledonary 
                 120.0 
                 132.8 
               
               
                 Height in cm. 
                 node to terminal 
               
               
                 Leaf: upper 
               
               
                 most, fully 
               
               
                 expanded 
               
               
                 Type 
                 normal, sub-okra, 
                 normal 
                 normal 
               
               
                   
                 okra, super-okra 
               
               
                 Pubescence 
                 absent, sparse, 
                 sparse 
                 sparse 
               
               
                   
                 medium, dense 
               
               
                 Nectaries 
                 present, absent 
                 present 
                 present 
               
               
                 Stem Pubescence 
                 glabrous, inter- 
                 inter- 
                 inter- 
               
               
                   
                 mediate, hairy 
                 mediate 
                 mediate 
               
               
                 Glands 
                 absent, sparse, 
               
               
                 (Gossypol) 
                 normal, more than 
               
               
                   
                 normal 
               
               
                 Leaf 
                   
                 normal 
                 normal 
               
               
                 Stem 
                   
                 normal 
                 normal 
               
               
                 Calyx lobe 
                 (normal is absent) 
                 normal 
                 normal 
               
               
                 Flower 
               
               
                 Petals 
                 cream, yellow 
                 cream 
                 cream 
               
               
                 Pollen 
                 cream, yellow 
                 cream 
                 cream 
               
               
                 Petal Spot 
                 present, absent 
                 absent 
                 absent 
               
               
                 Peduncle Length 
                   
                 23.7 
                 20.2 
               
               
                 (mm) 
               
               
                 Stigma Distance 
                   
                 5.7 
                 3.4 
               
               
                 above Stamens 
               
               
                 (mm) 
               
               
                 Seed 
               
               
                 Seed Index 
                 g/100 seed 
                 10.1 
                 8.9 
               
               
                   
                 fuzzy basis 
               
               
                 Lint Index 
                 g lint/100 seeds 
                 6.8 
                 6.9 
               
               
                 Boll 
               
               
                 Gin 
                 picked, stripped 
                 picked 
               
               
                 Lint % 
                   
                 36.1 
                 43.1 
               
               
                 Number of Seeds 
                   
                 28.2 
                 28.1 
               
               
                 per Boll 
               
               
                 Grams Seed 
                   
                 4.8 
                 4.6 
               
               
                 Cotton per Boll 
               
               
                 Number of Locules 
                   
                 4.0 
                 4.2 
               
               
                 per Boll 
               
               
                 Boll Type 
                 storm proof, storm 
                 open 
                 open 
               
               
                   
                 resistant, open 
               
               
                 Fiber Properties 
               
               
                 Method 
                 HVI or Other 
                 HVI 
               
               
                 Length 
                 inches, 2.5% SL 
                 1.17 
                 1.15 
               
               
                 Uniformity 
                 % 
                 84.7 
                 83.0 
               
               
                 Strength, T1 
                 g/tex 
                 29.2 
                 26.5 
               
               
                 Elongation, E1 
                 % 
                 6.6 
                 7.0 
               
               
                 Micronaire 
                   
                 4.3 
                 4.4 
               
               
                 Diseases, Insects 
                 susceptible = S, 
               
               
                 and Pests 
                 moderately 
               
               
                   
                 susceptible = MS 
               
               
                   
                 moderately 
               
               
                   
                 resistant = MR, 
               
               
                   
                 resistant = R 
               
               
                 Bacterial 
                   
                 R 
               
               
                 Blight race 1 
               
               
                 Bacterial 
                   
                 R 
               
               
                 Blight race 2 
               
               
                 Bacterial 
                   
                 R 
               
               
                 Blight Race 18 
               
               
                   Verticillium  Wilt 
                   
                 MR 
               
               
                 Bollworm 
                   
                 R 
               
               
                 Cotton Leafworm 
                   
                 R 
               
               
                 Fall Armyworm 
                   
                 R 
               
               
                 Pink Bollworm 
                   
                 R 
               
               
                 Tobacco Budworm 
                   
                 R 
               
               
                   
               
             
          
         
       
     
     
       
         
               
             
               
               
             
               
               
               
             
           
               
                 TABLE 2 
               
             
             
               
                   
               
               
                 Distinguishing Characteristics 
               
             
          
           
               
                   
                 Variety 
               
             
          
           
               
                 Characteristic 
                 FM 991B2R 
                 DP 555BG/RR 
               
               
                   
               
               
                 Bollgard II gene (Cry2Ab) 
                 Present 
                 Absent 
               
               
                 Height to first Fruiting Branch (cm) 
               
               
                 Mean 
                 17.3 
                 23.3 
               
               
                 Range 
                 15.3–18.7 
                 20.6–26.0 
               
               
                 LSD (1%) 
                 2.47 
               
               
                 Significance 
                   
                 P &lt; 0.01 
               
               
                 No. of Nodes to 1st Fruiting Branche 
                 6.5 
                 7.3 
               
               
                 Mean 
                 6.1–6.7 
                 6.9–7.9 
               
               
                 Range 
                 0.39 
               
               
                 LSD (1%) 
                   
                 P &lt; 0.01 
               
               
                 Significance 
               
               
                 Plant Height (cm) 
               
               
                 Mean 
                 120.0 
                 132.8 
               
               
                 Range 
                 106.8–130.3 
                 119–141 
               
               
                 LSD (1%) 
                 7.62 
               
               
                 Significance 
                   
                 P &lt; 0.01 
               
               
                 Peduncle Length (mm) 
               
               
                 Mean 
                 23.7 
                 20.2 
               
               
                 Range 
                 21.0–25.2 
                 16.8–25.0 
               
               
                 LSD (1%) 
                 2.57 
               
               
                 Significance 
                   
                 P &lt; 0.01 
               
               
                 Stigma Distance above Stamens (mm) 
               
               
                 Mean 
                 4.5 
                 3.4 
               
               
                 Range 
                 3.9–5.0 
                 2.0–4.8 
               
               
                 LSD (1%) 
                 0.82 
               
               
                 Significance 
                   
                 P &lt; 0.01 
               
               
                 Lint Percentage 
               
               
                 Mean 
                 36.1 
                 43.1 
               
               
                 Range 
                 33.8.37.1 
                 41.7–44.7 
               
               
                 LSD (1%) 
                 1.34 
               
               
                 Significance 
                   
                 P &lt; 0.01 
               
               
                   
               
             
          
         
       
     
     EXAMPLE 2 
     A variety essentially derived from FM 991B2R is obtained by the process of the transgression of the Event LLCotton 25 (USDA-APHIS petition 02-042-01p, see also U.S. Pat. No. 6,818,807, the disclosure of each of which is herein incorporated by reference in their entireties), into plants of the variety FM 991B2R via the method of recurrent backcrossing and selecting the plants which express the characteristics of FM 991B2R combined with the resistance to the herbicide glufosinate. 
     EXAMPLE 3 
     A variety essentially derived from FM 991B2R is obtained by the process of the transgression of the Event LLCotton 25 via genetic engineering in regenerable cells or tissue of FM 991B2R and the subsequent selection of regenerated plants, which express the characteristics of FM 991B2R combined with the resistance to the herbicide glufosinate. 
     EXAMPLE 4 
     A variety essentially derived from FM 991B2R is obtained by the selection of an induced or natural occurring mutant plant or off-type plant from plants of FM 991B2R, which plant retains the expression of the phenotypic characteristics of FM 991B2R and differs only from FM 991B2R in the expression of one, two, three, four or five of the characteristics as listed in table 1, and when grown side by side with FM 991B2R on one or two locations in one or two growing seasons. 
     CITED REFERENCES 
     F. N. Briggs and P. F. Knowles, “Introduction to Plant Breeding.” Rheinhold Publishing Corporation, 1967. 
     H. F. Sakhanoko et al., “Induction of Somatic Embryogenesis and Plant Regeneration in Select Georgia and Pee Dee Cotton Lines.” Crop Science 44:2199-2205 (2004). 
     Umbeck et al., “Genetic Engineering of Cotton Plants and Lines.” Published patent Application Number EP0290355 (1988). 
     Reynaerts et al., “Improved Method for  Agrobacterium  Mediated Transformation of Cotton.” International Publication Number WO 00/71733 (2000). 
     P. Stam, “Marker-assisted introgression: speed at any cost?” Proceedings of the Eucarpia Meeting on Leafy Vegetable Genetics and Breeding, Noordwijkerhout, The Netherlands, 19-21 March 2003. Eds. Th. J. L. van Hintum, A. Lebeda, D. Pink, J. W. Schut. P117-124 (2003). 
     Trolinder et al. “Herbicide tolerant cotton plants having event EE-GH1.” U.S. Pat. No. 6,818,807 (2004). 
     Rangwala et al. “Cotton Event PV-GHGT07(1445) Compositions and Methods for Detection thereof.” U.S. Pat. No. 6,740,488 (2004). 
     Rangwala et al. “Cotton Event PV-GHGT07(1445) Compositions and Methods for Detection thereof.” Publication No. US 2004/0148666.