Abstract:
A method and composition for preventing prostate cancer and/or reducing PSA levels and/or alleviating the symptoms of BPH (Benign Prostatic Hyperplasia) or PIN (prostatic intraepithelial neoplasia) by administration of an effective amount of a mixture of flavopereirine and alstonine. A method and composition for treating low-grade prostate cancer and preventing the onset of metastatic disease and/or reducing the doubling time of PSA levels in men with positive biopsies showing relatively low Gleason scores and morphologies characteristic of non-invasive, slow-progressing prostate cancer. The flavopereirine and alstonine can be in the form of natural extracts derived from  Pao Pereira  and  Rauwolfia Vomitoria , respectively. Alternatively, these two active compounds can be administered in purified form. The composition can be in included in a kit along with instructions for use in a treatment regimen.

Description:
FIELD OF INVENTION 
       [0001]    This invention is in the field of the prevention and treatment of prostate cancer. More specifically, this invention relates to the use of flavopereirine and alstonine combinations in preventing and treating prostate cancer. 
       BACKGROUND OF THE INVENTION 
       [0002]    Prostate cancer is the most prevalent cancer in men. Diagnosis is based on a tissue biopsy, microscopic examination of a small section of the prostate. When the biopsy contains cancerous cells the therapeutic options generally include radio- and chemotherapies, hormone treatments as well as surgical removal of the prostate gland. It is well known that these treatments are frequently devastating for the patient and notwithstanding these therapies mortality from prostate cancer is high. 
         [0003]    There is an urgent need for agents that prevent the advancement of prostate cancer. Indeed, of the approximately one million prostate biopsies performed in this country every year, by far the majority are negative. Yet these men suffer a range of symptoms associated with elevated PSA levels and benign prostatic hyperplasia affecting urinary and sexual function. These large groups of men with BPH (Benign Prostatic Hyperplasia) or elevated PSA, or with PIN (prostatic intraepithelial neoplasia), but with negative biopsies, are precancerous: they are at high risk of developing an aggressive prostate cancer and there have been no effective preventive treatments. Agents that forestall or significantly delay the onset of cancer in this population have remarkable therapeutic value. 
         [0004]    Another large population of men is diagnosed with prostate cancer, but their biopsies reveal a low-grade form of the disease. This group is in the well-known ‘watchful waiting’ or ‘active surveillance’ category and when the rate of increase in their PSA levels is relatively rapid they are subject to the therapeutic interventions cited above. These large groups of men—those in watchful waiting and those with shorter PSA doubling times need agents that subdue their cancers, slow the rate of PSA increase, and prevent the progression of their cancers to a higher grade and more aggressive form. Such an agent would fill a major vacuum in healthcare for men. 
         [0005]    A third population of men undergo one of the traditional therapies in which case their recovery is assessed by monitoring the rate of change or the doubling time of their PSA levels which serve as an indicator of how rapidly their cancer is likely to return. An agent that is effective in reducing PSA levels and in moderating PSA doubling times could be used in combination with traditional therapies to help prevent the recurrence of prostate cancer thus significantly extending the life of this population of cancer patients. 
       SUMMARY OF THE INVENTION 
       [0006]    The present invention includes compositions and methods for alleviating or avoiding the onset of the symptoms associated with prostate cancer, for treating low-grade prostate cancers and preventing their progression to more advanced cancers, and for decreasing PSA doubling times and thus preventing or delaying metastatic prostate cancer. The composition includes a mixture of flavopereirine and alstonine. Flavopereirine and alstonine are present in defined ratios relative to each other. The flavopereirine and alstonine may be present as plant extracts. The extract containing flavopereirine is derived from  Pao Pereira  and the extract containing alstonine is derived from  Rauwolfia Vomitoria . The extract of flavopereirine to the extract of alstonine ratio is about 3-4:1 and is based on the active ingredients being in extract form, i.e.  Pao Pereira  extract to  Rauwolfia Vomitoria  extract. Alternatively the flavopereirine and alstonine may be present in purified chemical form, i.e. a combination of synthetic flavopereirine and synthetic alstonine. It is expected that the ratio will vary somewhat depending on the purity of the active ingredients in the case of the active ingredients being in extract form. The amounts are sufficient to induce apoptosis and disruption of the cell cycle and DNA damage response pathways in prostate cancer cells. 
         [0007]    Any conventional modes of administration for compounds of this type are envisioned. Currently, oral administration is contemplated. Typical modes of this type would include tablets and capsules. These could be packaged in kit form with written instructions to facilitate a treatment regimen. 
         [0008]    The population envisioned for treatment with the flavopereirine and alstonine mixture falls into three groups: 1) those subjects thought to be susceptible to prostate cancer; 2) those subjects with low-grade forms of prostate cancer with both low and high PSA doubling times; and 3) those subjects combining treatment with traditional medical therapies (specifically surgery, radiation, and cryo- and/or chemotherapy) Population number one could include such high-risk groups as males having a high PSA count, symptoms of BPH (Benign Prostatic Hyperplasia) or with PIN (prostatic intraepithelial neoplasia). This group will have negative biopsies and would be considered precancerous. The amount of the mixture is sufficient to alleviate the symptoms of BPH (Benign Prostatic Hyperplasia) or to lower elevated PSA levels over time. Population number two will have been diagnosed with prostate cancer and thus will be likely to have positive biopsies, but have non-aggressive tumors characterized by lower Gleason scores. This population will include men with relatively low rates of PSA increase as well as men with higher rates of PSA increase. The amount of the mixture is sufficient to slow the progression of prostate cancer to a more aggressive stage and to lower PSA levels and PSA doubling times. Population number three will have or will be undergoing some form of prostate cancer therapy (surgery, radiation, cryo- or chemotherapy). Used in combination with these therapies the amount of the mixture is sufficient to lower PSA doubling times. 
         [0009]    The invention also includes a novel combination of these two extracts in relative amounts to prevent prostate cancer or alleviate symptoms of BPH, especially in precancerous men including men diagnosed with prostatic intraepithelial neoplasia (PIN). The extracts exert beneficial effects without the side effects normally associated with anti-cancer treatments. e Precancerous cell that are becoming cancerous are selectively killed by the mixture and the normal cells are left unaffected. 
         [0010]    The invention also includes a method of preventing prostate carcinogenesis in patients having a precancerous precursor of prostate adenocarcinoma, elevated PSA, and Benign Prostatic Hyperplasia, but who do not as yet have prostate cancer. The invention further includes a method for reducing PSA levels and/or alleviating the symptoms of BPH. 
         [0011]    The invention also includes a method of treating men with localized (non-metastatic) prostate cancer to prevent the development of higher-grade tumors and more aggressive disease. The cancer cells are selectively killed by the mixture and the normal cells are left unaffected. 
         [0012]    The invention also includes a method of treating men with both long and short PSA doubling times and of lengthening the PSA doubling times. This treatment will help maintain the ‘watchful waiting’ status of men with long PSA doubling times and help prevent the onset of more aggressive or metastatic disease in men with short PSA doubling times. 
         [0013]    The invention further provides a method of administering to an individual an orally effective dosage of the extracts in capsule or tablet form. 
         [0014]    The invention provides a method for suppression or inhibition of prostate carcinogenesis, reduction of PSA levels, and reduction of the symptoms of BPH by administration of the preventive agent that consists of a combination of natural extracts derived from  Pao Pereira  and  Rauwolfia Vomitoria.    
         [0015]    The invention provides a mechanism of action for the preventive agent by induction of apoptosis and disruption of cell cycle and DNA damage response pathways in cancer cells. At optimal concentrations this mechanism is selective for cancer cells and it also contributes to the effectiveness of the preventive agent in reducing PSA levels and the symptoms of BPH. 
     
    
     
       BRIEF DESCRIPTION OF THE FIGURES 
         [0016]      FIG. 1  shows the results of LNCaP cell growth analyses following treatment with  Rauwolfia  and  Pao  extracts. 
           [0017]      FIG. 2  shows the induction of apoptosis in LNCaP cells by both  Rauwolfia  and  Pao  extracts after 24 hours in terms of PARP cleavage and Caspase-3 activity. 
           [0018]      FIG. 3  shows cell cycle analysis by FACS. 
           [0019]      FIG. 4  (A and B) shows the results of a prostate cell tumor growth experiment. 
           [0020]      FIG. 5  shows the results for the TUNEL staining assay. 
           [0021]      FIG. 6  shows the corresponding statistical data for the TUNEL staining assay. 
           [0022]      FIG. 7  shows the experimental results illustrating the suppression of LNCap tumor xenograft growth by  Rauwolfia.    
       
    
    
     DESCRIPTION OF THE INVENTION 
     Overview 
       [0023]    The present invention builds on the scientific research of M. Beljanski who first developed these extracts and demonstrated their selective activity against cancer cells in laboratory experiments and in animal studies. Beljanski provided evidence that the toxicity of these compounds is based on their interaction with the destabilized DNA that he found to be a primary characteristic of cancer cells. 
         [0024]    The work described herein supports the correlation of the extent of DNA damage in prostate cells with cancer of the prostate; this also supports the specific diagnosis of a precancerous condition of the prostate. Also described is work demonstrating the mechanism of action involves induction of apoptosis and disruption of the cell cycle and DNA damage response pathways in prostate cancer cells. This is shown both in tissue culture and in an animal xenograft model. Uncovering the differential mechanisms of action of flavopereirine and alstonine supports the concept of using a specific combination of the extracts to obtain an enhanced therapeutic effect. 
         [0025]    The classification of an intermediate or precancerous stage of prostate DNA damage indicated that these compounds would exert their toxic effect specifically on precancerous cells that are in transition to a cancerous state thereby preventing cancer in high-risk men and answering the urgent need of a very large patient population. These compounds also exert their toxic effect on cancer cells thereby preventing advancement to a higher grade and more aggressive form of prostate cancer. 
       Discovery of the Anti-Cancer Activity of  Pao Pereira  and  Rauwolfia Vomitoria  Extracts. 
       [0026]    The extract from  Pao Pereira  is prepared according to the protocol in U.S. Pat. No. 5,519,028. The extract from  Rauwolfia Vomitoria  is prepared according to the procedure in European Pat. No. EP-A-0 059 817. This procedure includes a partial purification step that eliminates toxic compounds (e.g. reserpine) from the product. 
         [0027]    The biochemical activity of these two extracts was first analyzed in an in vitro assay called the Oncotest (U.S. Pat. No. 4,264,729). This test was originally designed to screen compounds for their carcinogenic potential and is based on differences in the secondary structure of DNAs isolated from cancer cells and from normal cells (Beljanski,  IRCS Med. Sci.  1979. 7:476). Relative to purified normal DNA, purified cancer cell DNA exhibits greater chromicity (absorption of UV light) and greater activity when used as a template for in vitro DNA synthesis (Beljanski et al.,  Expl. Cell. Biol.,  1981.49:220-231. This indicates that the hydrogen bonds in the double helix of cancer DNA are reproducibly disrupted resulting in openings or loops that increase UV absorption and enhance enzymatic activity in vitro. This fundamental difference in structure, the greater strand separation characteristic of cancer DNA, was referred to as DNA destabilization (Beljanski, Proceedings of the international seminar: Traditional Medicine: a Challenge to the 21 st  Century. 1992. Oxford and IBH Publishing Co.) 
         [0028]    When known carcinogens are added to the Oncotest they promote a small increase in DNA synthesis with normal templates, but a much higher increase (5 to 10 fold) in DNA synthesis with cancer templates (Beljanski et al. Third NCI-EORTC Symposium on new drugs in cancer therapy. 1981. Institut Bordet, Bruxelles). Such compounds are thought to act by further opening the already comparatively relaxed structure of the cancer DNA thereby enhancing the access of enzymes to the template and enabling more DNA to be synthesized. 
         [0029]    When the  Pao  and  Rauwolfia  extracts are used in the Oncotest they have the opposite affect of a carcinogen: chromicity is diminished and DNA synthesis from cancer DNA templates is inhibited (Beljanski,  Expl. Cell. Biol.  1982.50: 79-87). Biochemical analysis of the extracts, including DNA binding assays, identified the active compounds as flavopereirine (in  Pao Pereira ) and alstonine (in  Rauwolfia Vomitoria ). Since the inhibitory effects shown by the extracts and their respective compounds is specific for cancer DNA, since there is no effect on normal DNA, and since very few of the hundreds of compounds that were screened gave these results, these extracts and the corresponding active compounds were further assessed for their potential as anti-cancer agents. 
         [0030]    The extracts were subjected to a long series of tests to examine their effect on cultured cancer cells (Beljanski et al.,  IRCS Med. Sci.  1984. 12:587-588), on animals with various kinds of cancer (Beljanski et al., Oncology, 1986. 43:198-203), and ultimately in numerous human case studies. The extracts showed several consistent and noteworthy properties. First, at optimal concentrations, they stopped the proliferation of cancer cell lines maintained in the laboratory, while sparing healthy cells. They were toxic to cancer cells in mice, but did no harm to the mice. They have proven to have anti-cancer effects on a range of human malignancies, but have shown no significant side effects. The activity of these extracts is selective to cancer DNA, to cancer cells, and to the tumors of organisms with cancer. There is evidence that this selectivity is due, in part, to their preferential entry into cancer cells because of changes that occur in the outer membranes of these cells (Beljanski et al.  International Journal of Oncology,  1996. 8:1143-1148). In the course of these experiments, the activity of the  Pao Pereira  extract (flavopereirine) (Beljanski,  Genetics and Mol. Biol.,  2000. 23, 1:29-33) and the  Rauwolfia Vomitoria  extract (alstonine) (unpublished data) were shown to inhibit the multiplication of a human prostate cancer cell line (PC3) to a similar extent. This result is significant in the context of the present invention because it demonstrates that both of the active compounds in the mixture are effective against cancer cells like PC-3 that do not respond to hormones. 
         [0031]    For the Oncotest, cancer DNA was isolated from a range of cancerous tissues or cells including breast, lung, ovary, neurocarcinoma, Ehrlich ascites tumor cells, KB and HeLa and L cells. Normal DNA was isolated from healthy breast, lung, ovary, brain, primary kidney, Vero cells, or SIRC cells in culture. Cell lines exposed to  Pao Pereira  extract included: u251, CCF-STTG-1 SW 1088 and C6 (brain); LoVo, CaCo-2 (colon); Sk-Hep 1 (liver); A498 (kidney); G-361 (skin); Es 2, Sw 626 (ovary); ZR-75-1, MCF-7 (breast); MIA PaCa2 (pancreas); PC3 (prostate); and TT (thymus). Normal cell lines tested with  Pao Pereira  include CRL 1656 (brain); CCD-18Co (colon); Clone 9 (liver); NRK-49F (kidney); and CCD-97Sk (skin). A summary of anecdotal information concerning the use of the extracts for treating human cancer patients can be found in Beljanski, Mirko Beljanski ou La Chronique d&#39;une “Fatwa” Scientifique, 2001, EVI Liberty Corp. 
       DNA Destabilization in Cancer Cells 
       [0032]    Recent studies by Malins et al. (Cancer related changes in prostate DNA as men age and early identification of metastasis in primary prostate tumors.  Proc. Natl. Acad. Sci.  2003 April 29 100(9): 5401-5406) using Fourier Transform Mass Spectroscopy have shown that the DNA in prostate cancer cells is indeed different from the DNA of normal prostate cells. Malins et al. refer to the difference as an increase in disorder in the cancer DNA relative to normal DNA and these changes correlate with age and apparently with exposure to carcinogens. Malins et al were also able to reproducibly detect an interim stage of disorder reflecting a precancerous condition of the prostate (Malins et al. Models of DNA structure achieve almost perfect discrimination between normal prostate, benign prostatic hyperplasia (BPH), and adenocarcinoma and have a high potential for predicting BPH and prostate cancer.  Proc. Natl. Acad. Sci.  1997 January 7; 94 (1): 259-264). This precancerous condition appears to correspond to the clinical condition known as prostatic intraepithelial neoplasia (PIN). 
         [0033]    Malins et al. report that the presence of carcinogens in the prostate leads to the production of free radicals that physically damage the DNA yielding ‘potentially billions of altered structures.’ Many of these alterations will affect the hydrogen bonds that stabilize the DNA duplex. This could produce the openings in the helix structure reported by Beljanski. 
       Novel Data Supporting the Application of  Pao Pereira  and  Rauwolfia  Extracts for the Prevention of Prostate Cancer 
       [0034]    1. Induction of Apoptosis: The Primary Mechanism of Action of Flavopereirine 
         [0035]    Flavopereirine and alstonine are structurally related alkaloids of the beta-carboline class. There is evidence that they enter the relatively open strands of the DNA in cancer cells and intercalate between adjacent nucleotides where their presence apparently interferes with the metabolism of DNA (Calvez, Flavopereirine is an intercalating agent for non-supercoiled DNA, Cancer Detection and Prevention 1998; 22(Supplement 1)). In effect, the destabilized DNA of the cancer cell is a target of these alkaloids and the end result is cell death. The toxicity for cancer cells was confirmed in new experiments showing that both the  Pao  and  Rauwolfia  extracts inhibit the growth of LNCaP prostate cancer cells in tissue culture ( FIG. 1 ). Moreover, these extracts exhibited significantly higher toxicity for LNCaP cells than for a primary culture of normal prostate cells (Data not shown). 
         [0036]    Our recent experiments have also shown that the primary mechanism of action of flavopereirine is the induction of apoptosis whereas alstonine has a relatively minor effect in the induction of this pathway Both the  Pao Pereira  extract and the  Rauwolfia Vomitoria  extract triggered apoptosis in the prostate cancer cell line LNCaP as determined by cleavage of PARP and induction of Caspase-3 [ FIG. 2 ], but the effect of the  Rauwolfia  extract was considerably less than the effect of to  Pao  extract. 
         [0037]    Apoptosis—also called programmed cell death—is a built-in biochemical pathway that triggers a cell to commit suicide. This pathway is normally used during development (e.g. in the development of the nervous system), by the immune system to protect the body from altered cells (e.g. cells infected by a virus), and as a cellular reaction to DNA damage (e.g. if not destroyed these cells can become cancerous). In many cancers, including many prostate cancers, the mechanism for inducing apoptosis is defective so cells that should normally be committing suicide survive and grow to form a tumor. Numerous cancer therapies actually function by inducing apoptosis in certain types of cancer cells. 
         [0038]    The induction of apoptosis is a central theme in the prostate cancer research community and the fact that the  Pao Pereira  and (to a significantly lower degree  Rauwolfia Vomitoria ) extracts exhibit this effect makes them extremely attractive as potential anti-prostate cancer agents. 
         [0039]    2. Potential for Synergistic Action of Flavopereirine and Alstonine Based on Differential Effects on the Apoptotic Pathway, on the Cell Cycle, and on the DNA Damage Response Pathway 
         [0040]    Although both extracts induce apoptosis and are toxic for prostate cancer cells, analysis of their action in the cell cycle by fluorescence activated cell sorting revealed different effects. As shown in  FIG. 3 , the  Pao Pereira  extract causes accumulation of LNCaP cells in SubG0 whereas  Rauwolfia Vomitoria  hinder progression of the cells from G1 to S. This result highlights a difference in the mechanism of action of the two extracts whose biochemical basis remains to be determined, though it is presumably based on the difference in the physical structures of flavopereirine and alstonine. One possibility is that they may induce apoptosis by different pathways (e.g. p53-dependent and p53-independent) and another is that they may function with somewhat different kinetics. Regardless of the reason, this difference in activity has therapeutic value: combinations of the two extracts have the potential to act in complementary fashion, that is, they are potentially synergistic in their anti-cancer effects. Combination therapies are often used in cancer treatment and are widely considered to offer significant advantages over the use of a single anti-cancer agent alone. 
         [0041]    To further explore the effect of these two extracts on prostate cancer cells a series of microarray assays were performed using arrays containing three different sets of human genes belonging to three different biochemical pathways: apoptosis, cell cycle, and DNA damage. In each case that RNAs for the assays were isolated from LNCaP cells that had been exposed to the extracts for 24 hours. The data obtained from these microarray experiments are referred to collectively as Table I. See Annex. Note that real time PCR was used to spot check the validity of the changes observed in these assays 
         [0042]    While the process of analyzing these data and synthesizing coherent descriptions of the effect of the  Pao  and  Rauwolfia  extracts on these complex pathways is ongoing and will require additional experiments, several observations can be made highlighting the fact that the two extracts induce different responses in all three microarrays. For example, in the DNA damage response array the  Pao  extract has a potentially significant effect on the expression of fourteen (14) genes (shown in bold) whereas the  Rauwolfia  extract has a potentially significant response on thirty (30) transcripts. Closer inspection reveals that both extracts have significant effects on the expression of just four common genes (ATM, DDIT3, ERCC1, and PDCD8). Even more remarkable is that of the four genes affected by both extracts only two are affected in the same way: DDIT3 and ERCC1 are both up-regulated. Analysis of the two other microarrays also reveals divergent results for the  Pao Pereira  and  Rauwolfia Vomitoria  extracts strongly substantiating the use of these extracts in combination in order to obtain their complementary effects. 
         [0043]    3. Flavopereirine and Alstonine are Active Against Prostate Cancer Tumors in a Xenograft Animal Model 
         [0044]    The ability of human tumors to grow as xenografts in athymic mice (e.g., Balb/c, nu/nu) provides a useful in vivo model for studying the biological response to therapies for human cancers. Since the first successful xenotransplantation of human tumors into athymic mice, many different human tumor cell lines (e.g., mammary, lung, genitourinary, gastro-intestinal, head and neck, glioblastoma, bone, and malignant melanomas) have been transplanted and successfully grown in nude mice. The use of this system enabled analysis of the activity of the compounds of the present invention on a prostate cancer xenograft of LNCaP cells in a mammal—a better model for humans than cell-based assays. A number of tests may be used to determine the level of activity, specificity and effect of the compounds and in this case tumor volume and immunohistochemistry were the endpoints. 
         [0045]    A graph of the results of the xenograft experiment using the  Pao Pereira  extract, with measurement of tumor size (cubic millimeters) plotted against time (days), are shown if  FIG. 4A . Daily doses of 10 and 20 mg/kg of  Pao Pereira  extract caused a statistically significant reduction in tumor volume compared to control animals and to animals treated with 50 mg/kg per day. The log transformation shown in  FIG. 4B  clearly shows that the results fall into two classes. A graph of the results of the xenograft experiment using the  Rauwolfia Vomitoria  extract is shown in  FIG. 7 . In this case all three doses of the extract caused statistically significant reduction in tumor volume relative to the controls [p&lt;0.0001)]. Finally, data from two immunohistochemical assays using sections of the tumors removed from the animals confirmed that the mechanism of action of  Pao  is the induction of apoptosis which is dosage dependent. The results for the TUNEL staining assay are shown in  FIG. 5  and the corresponding statistical data are shown in  FIG. 6 . Consistent results were obtained in the BrdU staining assay [Data not shown]. Similar immunohistochemistry for the  Rauwolfia Vomitoria  experiment is currently underway. 
         [0046]    The dramatic effect of these extracts in reducing tumor volume must also be considered in light of the remarkable fact that the health of the animals in these experiments is not otherwise compromised in any adverse way. For example, the weights of treated and control mice show no significant differences throughout the course of the experiment. The anti-prostate cancer effects of both extracts together with the absence of negative side effects provide a strong scientific basis for the claims described in the present invention. 
       Formulation and Administration 
       [0047]    The flavopereirine and alstonine combinations can be formulated as pharmaceutical compositions. The compositions can be included in pharmaceutical kits. Depending on the subject to be treated, the mode of administration, and the type of treatment desired—e.g, prevention, prophylaxis, therapy; the flavopereirine and alstonine combinations are formulated in ways consistent with these parameters. A summary of such techniques is found in Remington&#39;s Pharmaceutical Sciences, latest edition, Mack Publishing Co., Easton, Pa. 
         [0048]    In general, for use in treatment or prophylaxis, the mixture of flavopereirine and alstonine may be used alone or in combination with other chemotherapeutic agents compounds. The mixture of flavopereirine and alstonine can be administrated singly or in combination with other pharmaceutically active components, and in single or multiple administrations. The mixture formulation may be prepared in a manner suitable for systemic administration. Systemic formulations include those designed for injection, e.g. intramuscular, intravenous or subcutaneous injection, or may be prepared for transdermal, transmucosal, or oral administration. The formulation will generally include a diluent as well as, in some cases, adjuvants, buffers, preservatives and the like. The flavopereirine and alstonine mixture can be administered also in liposomal compositions or as microemulsions using conventional techniques. 
         [0049]    If orally administered, the flavopereirine and alstonine combination of the invention can be protected from degradation in the stomach using a suitable enteric coating. 
         [0050]    The manner of administration and formulation of the mixture useful in the invention will depend on the nature of the condition, the severity of the condition, the particular subject to be treated, and the judgment of the practitioner, formulation will depend on mode of administration As the mixture of the invention involve small molecules, it is conveniently administered by oral administration by compounding them with suitable pharmaceutical excipients so as to provide tablets, capsules, syrups, and the like. Suitable formulations for oral administration may also include minor components such as buffers, flavoring agents and the like. Typically, the amount of active ingredient in the formulations will be in the range of 1%-99% of the total formulation, but wide variation is permitted depending on the carrier. Suitable carriers include sucrose, pectin, magnesium stearate, lactose, peanut oil, olive oil, water, and the like. 
         [0051]    The mixture may also be administered by injection, including intravenous, intramuscular, subcutaneous or intraperitoneal injection. Typical formulations for such use are liquid formulations in isotonic vehicles such as Hank&#39;s solution or Ringer&#39;s solution. 
         [0052]    Suitable alternative formulations also include liposomal formulations, slow-release formulations, and the like. 
         [0053]    Any suitable formulation may be used. A compendium of art-known formulations is found in Remington&#39;s Pharmaceutical Sciences, latest edition, Mack Publishing Company, Easton, Pa. Reference to this manual is routine in the art. 
         [0054]    The dosages of the compounds of the invention will depend on a number of factors which will vary from patient to patient. 
         [0055]    Oral dosage forms include capsules and tablets. Capsule or tablets can be easily formulated and can be made easy to swallow. Tablets may contain suitable carriers, binders, lubricants, diluents, disintegrating agents, coloring agents, flavoring agents, flow-inducing agents, or melting agents. A tablet may be made by compression or molding, optionally with one or more additional ingredients. Compressed tables may be prepared by compressing the active ingredient in a free flowing form (e.g., powder, granules) optionally mixed with a binder (e.g., gelatin, hydroxypropylmethylcellulose), lubricant, inert diluent, preservative, disintegrant (e.g., sodium starch glycolate, cross-linked carboxymethyl cellulose) surface-active or dispersing agent. Suitable binders include starch, gelatin, natural sugars such as glucose or beta-lactose, corn sweeteners, natural and synthetic gums such as acacia, tragacanth, or sodium alginate, carboxymethylcellulose, polyethylene glycol, waxes, or the like. Lubricants used in these dosage forms include sodium oleate, sodium stearate, magnesium stearate, sodium benzoate, sodium acetate, sodium chloride, or the like. Disintegrators include, for example, starch, methyl cellulose, agar, bentonite, xanthan gum, or the like. Molded tablets may be made by molding in a suitable machine a mixture of the powdered active ingredient moistened with an inert liquid diluent. 
         [0056]    The tablets may optionally be coated or scored and may be formulated so as to provide slow- or controlled-release of the active ingredient. Tablets may also optionally be provided with an enteric coating to provide release in parts of the gut other than the stomach. 
         [0057]    As noted above the flavopereirine and alstonine mixture can be included in a kit form. The mixture would be formulated for use as a pharmaceutical. The kits can comprise one or more containers containing the pharmaceutical form of the mixture, which comprises a therapeutically effective amount of the mixture in unit dosage form. Such kits can further include, if desired, one or more of various conventional pharmaceutical kit components, such as, for example, containers with one or more pharmaceutically acceptable carriers, additional containers, etc., as will be readily apparent to those skilled in the art. Instructions, such as printed instructions for example, either as inserts or as labels, the instruction indicating quantities of the components to be administered, guidelines for administration, and/or guidelines for further mixing with additional components, can also be included in the kit. 
       Tables 
       [0058]      
         [0000]    
       
         
               
               
               
             
               
               
               
               
             
               
               
               
               
               
               
               
             
           
               
                   
                   
               
             
             
               
                   
                 Project 
                 Pao Pereira Apoptosis 100 ug/ml Pao 24 hr/LNCaP 
               
               
                   
                 Catalog # 
                 Oligo GEArray ® Human Apoptosis Microarray 
               
               
                   
                 Density 
                 Average 
               
               
                   
                 Clover 
                 Clover Off 
               
               
                   
                 Total Arrays 
                 2 
               
               
                   
                 Total Groups 
                 2 
               
               
                   
                 Background 
                 Empty spots: positions 117, 114, 116 
               
               
                   
                 Normalization 
                 Selected genes: positions 126 
               
             
          
           
               
                   
                 Array Name 
                 C apoptosis light.tif 
                 T apoptosis.tif 
               
               
                   
                 Assigned Group 
                 Group 1 
                 Group 2 
               
               
                   
                   
               
             
          
           
               
                 Position 
                 UniGene 
                 RefSeq # 
                 Symbol 
                 Group 2 
                 Group 1 
                 Group 2/Group 1 
               
               
                   
               
               
                 1 
                 Hs.546292 
                 NM_002954 
                 RPS27A 
                 1.462 
                 1.029 
                 1.422 
               
               
                 2 
                 Hs.431048 
                 NM_005157 
                 ABL1 
                 0.130 
                 0.201 
                 0.648 
               
               
                 3 
                 Hs.525622 
                 NM_005163 
                 AKT1 
                 0.494 
                 0.607 
                 0.814 
               
               
                 4 
                 Hs.552567 
                 NM_001160 
                 
                   APAF1 
                 
                 
                   0.019 
                 
                 
                   −0.003 
                 
                 
                   −6.013 
                 
               
               
                 5 
                 Hs.370254 
                 NM_004322 
                 BAD 
                 0.024 
                 0.027 
                 0.896 
               
               
                 6 
                 Hs.377484 
                 NM_004323 
                 
                   BAG1 
                 
                 
                   0.040 
                 
                 
                   0.084 
                 
                 
                   0.472 
                 
               
               
                 7 
                 Hs.523309 
                 NM_004281 
                 BAG3 
                 0.137 
                 0.117 
                 1.172 
               
               
                 8 
                 Hs.194726 
                 NM_004874 
                 BAG4 
                 0.006 
                 −0.011 
                 −0.491 
               
               
                 9 
                 Hs.485139 
                 NM_001188 
                 BAK1 
                 0.187 
                 0.269 
                 0.695 
               
               
                 10 
                 Hs.159428 
                 NM_004324 
                 
                   BAX 
                 
                 
                   0.026 
                 
                 
                   −0.002 
                 
                 
                   −16.620 
                 
               
               
                 11 
                 Hs.549082 
                 NM_003921 
                 
                   BCL10 
                 
                 
                   0.016 
                 
                 
                   −0.001 
                 
                 
                   −27.393 
                 
               
               
                 12 
                 Hs.150749 
                 NM_000633 
                 
                   BCL2 
                 
                 
                   0.016 
                 
                 
                   −0.002 
                 
                 
                   −8.129 
                 
               
               
                 13 
                 Hs.227817 
                 NM_004049 
                 
                   BCL2A1 
                 
                 
                   0.012 
                 
                 
                   −0.007 
                 
                 
                   −1.853 
                 
               
               
                 14 
                 Hs.516966 
                 NM_138578 
                 BCL2L1 
                 0.094 
                 0.139 
                 0.680 
               
               
                 15 
                 Hs.283672 
                 NM_020396 
                 
                   BCL2L10 
                 
                 
                   0.013 
                 
                 
                   −0.009 
                 
                 
                   −1.464 
                 
               
               
                 16 
                 Hs.469658 
                 NM_006538 
                 
                   BCL2L11 
                 
                 
                   0.010 
                 
                 
                   −0.009 
                 
                 
                   −1.046 
                 
               
               
                 17 
                 Hs.289052 
                 NM_052842 
                 BCL2L12 
                 0.124 
                 0.325 
                 0.383 
               
               
                 18 
                 Hs.546359 
                 NM_015367 
                 BCL2L13 
                 0.131 
                 0.204 
                 0.642 
               
               
                 19 
                 Hs.410026 
                 NM_004050 
                 
                   BCL2L2 
                 
                 
                   0.015 
                 
                 
                   0.001 
                 
                 
                   25.098 
                 
               
               
                 20 
                 Hs.486542 
                 NM_014739 
                 BCLAF1 
                 0.021 
                 0.029 
                 0.726 
               
               
                 21 
                 Hs.435556 
                 NM_016561 
                 BFAR 
                 0.004 
                 −0.003 
                 −1.284 
               
               
                 22 
                 Hs.474150 
                 NM_001196 
                 BID 
                 0.128 
                 0.129 
                 0.993 
               
               
                 23 
                 Hs.475055 
                 NM_001197 
                 
                   BIK 
                 
                 
                   0.031 
                 
                 
                   0.006 
                 
                 
                   5.098 
                 
               
               
                 24 
                 Hs.519374 
                 NM_004536 
                 BIRC1 
                 0.002 
                 −0.013 
                 −0.174 
               
               
                 25 
                 Hs.503704 
                 NM_001166 
                 BIRC2 
                 0.046 
                 0.066 
                 0.698 
               
               
                 26 
                 Hs.127799 
                 NM_001165 
                 BIRC3 
                 0.013 
                 −0.012 
                 −1.136 
               
               
                 27 
                 Hs.356076 
                 NM_001167 
                 BIRC4 
                 0.006 
                 0.001 
                 6.777 
               
               
                 28 
                 Hs.514527 
                 NM_001168 
                 BIRC5 
                 0.610 
                 0.941 
                 0.648 
               
               
                 29 
                 Hs.150107 
                 NM_016252 
                 BIRC6 
                 0.008 
                 −0.003 
                 −2.277 
               
               
                 30 
                 Hs.256126 
                 NM_022161 
                 BIRC7 
                 0.005 
                 −0.011 
                 −0.441 
               
               
                 31 
                 Hs.348263 
                 NM_033341 
                 
                   BIRC8 
                 
                 
                   0.011 
                 
                 
                   −0.012 
                 
                 
                   −0.912 
                 
               
               
                 32 
                 Hs.145726 
                 NM_001205 
                 
                   BNIP1 
                 
                 
                   0.039 
                 
                 
                   0.007 
                 
                 
                   5.728 
                 
               
               
                 33 
                 Hs.283454 
                 NM_004330 
                 BNIP2 
                 0.016 
                 0.010 
                 1.541 
               
               
                 34 
                 Hs.79428 
                 NM_004052 
                 BNIP3 
                 1.091 
                 0.942 
                 1.159 
               
               
                 35 
                 Hs.131226 
                 NM_004331 
                 BNIP3L 
                 0.139 
                 0.163 
                 0.853 
               
               
                 36 
                 Hs.293753 
                 NM_032515 
                 BOK 
                 0.071 
                 0.067 
                 1.064 
               
               
                 37 
                 Hs.490366 
                 NM_004333 
                 
                   BRAF 
                 
                 
                   0.014 
                 
                 
                   0.001 
                 
                 
                   13.023 
                 
               
               
                 38 
                 Hs.57973 
                 NM_014550 
                 
                   CARD10 
                 
                 
                   0.022 
                 
                 
                   0.003 
                 
                 
                   7.058 
                 
               
               
                 39 
                 Hs.300355 
                 NM_032415 
                 CARD11 
                 0.005 
                 −0.000 
                 −11.164 
               
               
                 40 
                 Hs.23248 
                 NM_021209 
                 CARD12 
                 0.004 
                 −0.015 
                 −0.266 
               
               
                 41 
                 Hs.550529 
                 NM_024110 
                 CARD14 
                 0.008 
                 −0.007 
                 −1.258 
               
               
                 42 
                 Hs.135201 
                 NM_022162 
                 CARD15 
                 0.011 
                 0.007 
                 1.576 
               
               
                 43 
                 Hs.405153 
                 NM_006092 
                 CARD4 
                 0.007 
                 −0.008 
                 −0.791 
               
               
                 44 
                 Hs.200242 
                 NM_032587 
                 CARD6 
                 0.005 
                 0.003 
                 1.868 
               
               
                 45 
                 Hs.446146 
                 NM_014959 
                 CARD8 
                 0.002 
                 −0.001 
                 −2.515 
               
               
                 46 
                 Hs.271815 
                 NM_022352 
                 
                   CARD9 
                 
                 
                   0.013 
                 
                 
                   −0.004 
                 
                 
                   −3.092 
                 
               
               
                 47 
                 Hs.2490 
                 NM_033292 
                 
                   CASP1 
                 
                 
                   0.012 
                 
                 
                   −0.004 
                 
                 
                   −2.743 
                 
               
               
                 48 
                 Hs.5353 
                 NM_001230 
                 
                   CASP10 
                 
                 
                   0.016 
                 
                 
                   −0.006 
                 
                 
                   −2.506 
                 
               
               
                 49 
                 Hs.248226 
                 NM_012114 
                 
                   CASP14 
                 
                 
                   0.011 
                 
                 
                   −0.009 
                 
                 
                   −1.273 
                 
               
               
                 50 
                 Hs.368982 
                 NM_001224 
                 CASP2 
                 0.123 
                 0.212 
                 0.581 
               
               
                 51 
                 Hs.141125 
                 NM_004346 
                 
                   CASP3 
                 
                 
                   0.016 
                 
                 
                   −0.003 
                 
                 
                   −4.959 
                 
               
               
                 52 
                 Hs.138378 
                 NM_001225 
                 CASP4 
                 0.137 
                 0.189 
                 0.725 
               
               
                 53 
                 Hs.213327 
                 NM_004347 
                 CASP5 
                 0.008 
                 −0.003 
                 −2.563 
               
               
                 54 
                 Hs.3280 
                 NM_001226 
                 CASP6 
                 0.020 
                 0.012 
                 1.696 
               
               
                 55 
                 Hs.9216 
                 NM_001227 
                 CASP7 
                 0.030 
                 0.027 
                 1.115 
               
               
                 56 
                 Hs.369736 
                 NM_001228 
                 CASP8 
                 −0.000 
                 −0.008 
                 0.027 
               
               
                 57 
                 Hs.329502 
                 NM_001229 
                 CASP9 
                 0.049 
                 0.068 
                 0.725 
               
               
                 58 
                 Hs.390736 
                 NM_003879 
                 CFLAR 
                 0.009 
                 −0.005 
                 −1.687 
               
               
                 59 
                 Hs.249129 
                 NM_001279 
                 
                   CIDEA 
                 
                 
                   0.013 
                 
                 
                   0.004 
                 
                 
                   3.523 
                 
               
               
                 60 
                 Hs.448590 
                 NM_014430 
                 
                   CIDEB 
                 
                 
                   0.013 
                 
                 
                   0.009 
                 
                 
                   1.446 
                 
               
               
                 61 
                 Hs.38533 
                 NM_003805 
                 CRADD 
                 0.115 
                 0.176 
                 0.652 
               
               
                 62 
                 Hs.380277 
                 NM_004938 
                 DAPK1 
                 0.006 
                 0.001 
                 6.290 
               
               
                 63 
                 Hs.237886 
                 NM_014326 
                 
                   DAPK2 
                 
                 
                   0.021 
                 
                 
                   0.004 
                 
                 
                   5.061 
                 
               
               
                 64 
                 Hs.484782 
                 NM_004401 
                 DFFA 
                 0.040 
                 0.025 
                 1.578 
               
               
                 65 
                 Hs.133089 
                 NM_004402 
                 
                   DFFB 
                 
                 
                   0.040 
                 
                 
                   0.082 
                 
                 
                   0.490 
                 
               
               
                 66 
                 Hs.86131 
                 NM_003824 
                 FADD 
                 0.167 
                 0.241 
                 0.690 
               
               
                 67 
                 Hs.80409 
                 NM_001924 
                 GADD45A 
                 0.177 
                 0.129 
                 1.377 
               
               
                 68 
                 Hs.248114 
                 NM_000514 
                 GDNF 
                 0.003 
                 0.007 
                 0.431 
               
               
                 69 
                 Hs.87247 
                 NM_003806 
                 HRK 
                 0.005 
                 −0.004 
                 −1.276 
               
               
                 70 
                 Hs.20573 
                 NM_000875 
                 IGF1R 
                 −0.002 
                 0.003 
                 −0.701 
               
               
                 71 
                 Hs.36 
                 NM_000595 
                 LTA 
                 0.005 
                 0.004 
                 1.105 
               
               
                 72 
                 Hs.1116 
                 NM_002342 
                 LTBR 
                 0.786 
                 0.751 
                 1.046 
               
               
                 73 
                 Hs.532826 
                 NM_021960 
                 MCL1 
                 0.459 
                 0.605 
                 0.758 
               
               
                 74 
                 Hs.513667 
                 NM_003946 
                 NOL3 
                 0.246 
                 0.317 
                 0.776 
               
               
                 75 
                 Hs.499094 
                 NM_013258 
                 
                   PYCARD 
                 
                 
                   0.011 
                 
                 
                   0.005 
                 
                 
                   2.128 
                 
               
               
                 76 
                 Hs.519842 
                 NM_003804 
                 RIPK1 
                 0.005 
                 0.002 
                 2.240 
               
               
                 77 
                 Hs.103755 
                 NM_003821 
                 
                   RIPK2 
                 
                 
                   0.072 
                 
                 
                   0.149 
                 
                 
                   0.485 
                 
               
               
                 78 
                 Hs.241570 
                 NM_000594 
                 TNF 
                 0.004 
                 0.004 
                 0.807 
               
               
                 79 
                 Hs.401745 
                 NM_003844 
                 TNFRSF10A 
                 0.031 
                 0.023 
                 1.376 
               
               
                 80 
                 Hs.521456 
                 NM_003842 
                 TNFRSF10B 
                 0.985 
                 0.921 
                 1.070 
               
               
                 81 
                 Hs.119684 
                 NM_003841 
                 TNFRSF10C 
                 0.016 
                 0.008 
                 1.991 
               
               
                 82 
                 Hs.213467 
                 NM_003840 
                 TNFRSF10D 
                 0.006 
                 0.007 
                 0.927 
               
               
                 83 
                 Hs.81791 
                 NM_002546 
                 
                   TNFRSF11B 
                 
                 
                   0.024 
                 
                 
                   −0.000 
                 
                 
                   −67.929 
                 
               
               
                 84 
                 Hs.355899 
                 NM_016639 
                 TNFRSF12A 
                 0.020 
                 0.029 
                 0.692 
               
               
                 85 
                 Hs.512898 
                 NM_003820 
                 TNFRSF14 
                 0.009 
                 −0.002 
                 −4.653 
               
               
                 86 
                 Hs.149168 
                 NM_018647 
                 TNFRSF19 
                 0.003 
                 −0.005 
                 −0.515 
               
               
                 87 
                 Hs.279594 
                 NM_001065 
                 TNFRSF1A 
                 0.107 
                 0.180 
                 0.595 
               
               
                 88 
                 Hs.256278 
                 NM_001066 
                 TNFRSF1B 
                 0.006 
                 −0.001 
                 −4.329 
               
               
                 89 
                 Hs.443577 
                 NM_014452 
                 
                   TNFRSF21 
                 
                 
                   0.012 
                 
                 
                   −0.007 
                 
                 
                   −1.676 
                 
               
               
                 90 
                 Hs.462529 
                 NM_003790 
                 TNFRSF25 
                 0.032 
                 0.034 
                 0.950 
               
               
                 91 
                 Hs.472860 
                 NM_001250 
                 CD40 
                 0.002 
                 −0.007 
                 −0.228 
               
               
                 92 
                 Hs.244139 
                 NM_152877 
                 FAS 
                 0.006 
                 −0.009 
                 −0.673 
               
               
                 93 
                 Hs.434878 
                 NM_003823 
                 TNFRSF6B 
                 0.050 
                 0.082 
                 0.613 
               
               
                 94 
                 Hs.355307 
                 NM_001242 
                 TNFRSF7 
                 0.006 
                 −0.003 
                 −2.164 
               
               
                 95 
                 Hs.193418 
                 NM_001561 
                 TNFRSF9 
                 0.005 
                 0.001 
                 7.460 
               
               
                 96 
                 Hs.478275 
                 NM_003810 
                 TNFSF10 
                 0.005 
                 −0.002 
                 −2.889 
               
               
                 97 
                 Hs.129708 
                 NM_003807 
                 TNFSF14 
                 −0.001 
                 0.001 
                 −1.310 
               
               
                 98 
                 Hs.248197 
                 NM_005092 
                 TNFSF18 
                 0.006 
                 −0.002 
                 −2.723 
               
               
                 99 
                 Hs.652 
                 NM_000074 
                 CD40LG 
                 0.003 
                 −0.005 
                 −0.555 
               
               
                 100 
                 Hs.2007 
                 NM_000639 
                 FASLG 
                 0.002 
                 −0.005 
                 −0.368 
               
               
                 101 
                 Hs.501497 
                 NM_001252 
                 TNFSF7 
                 0.003 
                 0.004 
                 0.754 
               
               
                 102 
                 Hs.494901 
                 NM_001244 
                 TNFSF8 
                 0.006 
                 −0.007 
                 −0.823 
               
               
                 103 
                 Hs.1524 
                 NM_003811 
                 TNFSF9 
                 0.003 
                 −0.004 
                 −0.658 
               
               
                 104 
                 Hs.408312 
                 NM_000546 
                 
                   TP53 
                 
                 
                   0.009 
                 
                 
                   0.026 
                 
                 
                   0.368 
                 
               
               
                 105 
                 Hs.523968 
                 NM_005426 
                 TP53BP2 
                 0.007 
                 0.006 
                 1.239 
               
               
                 106 
                 Hs.192132 
                 NM_005427 
                 TP73 
                 0.000 
                 −0.006 
                 −0.073 
               
               
                 107 
                 Hs.137569 
                 NM_003722 
                 TP73L 
                 0.004 
                 −0.005 
                 −0.808 
               
               
                 108 
                 Hs.460996 
                 NM_003789 
                 TRADD 
                 0.205 
                 0.246 
                 0.835 
               
               
                 109 
                 Hs.531251 
                 NM_005658 
                 TRAF1 
                 0.002 
                 −0.000 
                 −5.206 
               
               
                 110 
                 Hs.522506 
                 NM_021138 
                 
                   TRAF2 
                 
                 
                   0.001 
                 
                 
                   0.016 
                 
                 
                   0.078 
                 
               
               
                 111 
                 Hs.510528 
                 NM_003300 
                 TRAF3 
                 0.026 
                 0.029 
                 0.894 
               
               
                 112 
                 Hs.8375 
                 NM_004295 
                 TRAF4 
                 0.434 
                 0.614 
                 0.707 
               
               
                 113 
                 Hs.523930 
                 NM_004619 
                 TRAF5 
                 −0.002 
                 −0.005 
                 0.356 
               
               
                 114 
                   
                   
                 Blank 
                 0.001 
                 −0.002 
                 −0.346 
               
               
                 115 
                 N/A 
                 L08752 
                 PUC18 
                 0.000 
                 −0.002 
                 −0.209 
               
               
                 116 
                   
                   
                 Blank 
                 0.001 
                 0.001 
                 1.087 
               
               
                 117 
                   
                   
                 Blank 
                 −0.002 
                 0.001 
                 −3.216 
               
               
                 118 
                 N/A 
                 N/A 
                 AS1R2 
                 0.000 
                 −0.007 
                 −0.016 
               
               
                 119 
                 N/A 
                 N/A 
                 AS1R1 
                 0.004 
                 0.002 
                 1.532 
               
               
                 120 
                 N/A 
                 N/A 
                 AS1 
                 0.013 
                 −0.005 
                 −2.927 
               
               
                 121 
                 Hs.544577 
                 NM_002046 
                 GAPD 
                 0.878 
                 1.014 
                 0.866 
               
               
                 122 
                 Hs.534255 
                 NM_004048 
                 B2M 
                 0.642 
                 0.645 
                 0.994 
               
               
                 123 
                 Hs.509736 
                 NM_007355 
                 HSPCB 
                 0.975 
                 1.006 
                 0.969 
               
               
                 124 
                 Hs.509736 
                 NM_007355 
                 HSPCB 
                 0.934 
                 1.039 
                 0.899 
               
               
                 125 
                 Hs.520640 
                 NM_001101 
                 ACTB 
                 1.008 
                 1.030 
                 0.979 
               
               
                 126 
                 Hs.520640 
                 NM_001101 
                 ACTB 
                 1.000 
                 1.000 
                 1.000 
               
               
                 127 
                 N/A 
                 N/A 
                 BAS2C 
                 0.055 
                 0.106 
                 0.517 
               
               
                 128 
                 N/A 
                 N/A 
                 BAS2C 
                 0.751 
                 0.922 
                 0.815 
               
               
                   
               
               
                 * As default, Group 1 is used as control group. 
               
             
          
         
       
     
         [0000]    
       
         
               
               
               
             
               
               
               
               
             
               
               
               
               
               
               
               
             
           
               
                   
                   
               
             
             
               
                   
                 Project 
                 Rauwolfia Apoptosis 500 ug/ml 24 hr LNCaP 
               
               
                   
                 Catalog # 
                 Oligo GEArray ® Human Apoptosis Microarray 
               
               
                   
                 Density 
                 Average 
               
               
                   
                 Clover 
                 Clover Off 
               
               
                   
                 Total Arrays 
                 2 
               
               
                   
                 Total Groups 
                 2 
               
               
                   
                 Background 
                 Empty spots: positions 117, 114, 116 
               
               
                   
                 Normalization 
                 Selected genes: positions 1, 121, 123, 124, 125, 126 
               
             
          
           
               
                   
                 Array Name 
                 Rauwolfia Apoptosis 
                 Rauwolfia Apoptosis Rx.tif 
               
               
                   
                 Assigned Group 
                 Group 1 
                 Group 2 
               
               
                   
                   
               
             
          
           
               
                 Position 
                 UniGene 
                 RefSeq # 
                 Symbol 
                 Group 2 
                 Group 1 
                 Group 2/Group 1 
               
               
                   
               
               
                 1 
                 Hs.546292 
                 NM_002954 
                 RPS27A 
                 0.4721 
                 0.4813 
                 0.9809 
               
               
                 2 
                 Hs.431048 
                 NM_005157 
                 ABL1 
                 −0.1908 
                 −0.0298 
                 6.4113 
               
               
                 3 
                 Hs.525622 
                 NM_005163 
                 AKT1 
                 −0.0315 
                 0.1583 
                 −0.1989 
               
               
                 4 
                 Hs.552567 
                 NM_001160 
                 APAF1 
                 0.3442 
                 −0.0376 
                 −9.1443 
               
               
                 5 
                 Hs.370254 
                 NM_004322 
                 BAD 
                 0.0596 
                 0.0600 
                 0.9930 
               
               
                 6 
                 Hs.377484 
                 NM_004323 
                 BAG1 
                 −0.0272 
                 0.1709 
                 −0.1590 
               
               
                 7 
                 Hs.523309 
                 NM_004281 
                 BAG3 
                 0.0421 
                 0.1204 
                 0.3493 
               
               
                 8 
                 Hs.194726 
                 NM_004874 
                 BAG4 
                 −0.1620 
                 0.1170 
                 −1.3841 
               
               
                 9 
                 Hs.485139 
                 NM_001188 
                 BAK1 
                 −0.0838 
                 0.0916 
                 −0.9154 
               
               
                 10 
                 Hs.159428 
                 NM_004324 
                 BAX 
                 −0.2322 
                 0.0343 
                 −6.7676 
               
               
                 11 
                 Hs.549082 
                 NM_003921 
                 BCL10 
                 −0.1444 
                 0.0383 
                 −3.7758 
               
               
                 12 
                 Hs.150749 
                 NM_000633 
                 BCL2 
                 −0.0527 
                 0.0706 
                 −0.7466 
               
               
                 13 
                 Hs.227817 
                 NM_004049 
                 BCL2A1 
                 −0.0477 
                 0.0874 
                 −0.5461 
               
               
                 14 
                 Hs.516966 
                 NM_138578 
                 BCL2L1 
                 −0.0831 
                 0.1577 
                 −0.5271 
               
               
                 15 
                 Hs.283672 
                 NM_020396 
                 BCL2L10 
                 −0.0871 
                 0.1131 
                 −0.7704 
               
               
                 16 
                 Hs.469658 
                 NM_006538 
                 BCL2L11 
                 −0.1348 
                 0.1165 
                 −1.1572 
               
               
                 17 
                 Hs.289052 
                 NM_052842 
                 BCL2L12 
                 −0.0735 
                 −0.0072 
                 10.2457 
               
               
                 18 
                 Hs.546359 
                 NM_015367 
                 BCL2L13 
                 −0.1448 
                 0.0797 
                 −1.8154 
               
               
                 19 
                 Hs.410026 
                 NM_004050 
                 BCL2L2 
                 −0.1769 
                 −0.0185 
                 9.5777 
               
               
                 20 
                 Hs.486542 
                 NM_014739 
                 BCLAF1 
                 0.0460 
                 0.0272 
                 1.6914 
               
               
                 21 
                 Hs.435556 
                 NM_016561 
                 BFAR 
                 −0.0530 
                 0.0711 
                 −0.7457 
               
               
                 22 
                 Hs.474150 
                 NM_001196 
                 BID 
                 −0.0215 
                 0.1373 
                 −0.1569 
               
               
                 23 
                 Hs.475055 
                 NM_001197 
                 BIK 
                 −0.1544 
                 0.1039 
                 −1.4857 
               
               
                 24 
                 Hs.519374 
                 NM_004536 
                 BIRC1 
                 −0.1686 
                 0.0748 
                 −2.2556 
               
               
                 25 
                 Hs.503704 
                 NM_001166 
                 BIRC2 
                 −0.0490 
                 0.0285 
                 −1.7181 
               
               
                 26 
                 Hs.127799 
                 NM_001165 
                 BIRC3 
                 −0.1521 
                 0.0251 
                 −6.0525 
               
               
                 27 
                 Hs.356076 
                 NM_001167 
                 BIRC4 
                 −0.1325 
                 0.0703 
                 −1.8852 
               
               
                 28 
                 Hs.514527 
                 NM_001168 
                 BIRC5 
                 −0.0861 
                 0.1853 
                 −0.4648 
               
               
                 29 
                 Hs.150107 
                 NM_016252 
                 BIRC6 
                 −0.1136 
                 0.0687 
                 −1.6536 
               
               
                 30 
                 Hs.256126 
                 NM_022161 
                 BIRC7 
                 −0.1444 
                 0.1063 
                 −1.3592 
               
               
                 31 
                 Hs.348263 
                 NM_033341 
                 BIRC8 
                 −0.1464 
                 0.0876 
                 −1.6711 
               
               
                 32 
                 Hs.145726 
                 NM_001205 
                 BNIP1 
                 −0.1315 
                 0.0661 
                 −1.9900 
               
               
                 33 
                 Hs.283454 
                 NM_004330 
                 BNIP2 
                 −0.0136 
                 0.0356 
                 −0.3812 
               
               
                 34 
                 Hs.79428 
                 NM_004052 
                 BNIP3 
                 0.1415 
                 0.2796 
                 0.5059 
               
               
                 35 
                 Hs.131226 
                 NM_004331 
                 BNIP3L 
                 0.0013 
                 0.0934 
                 0.0142 
               
               
                 36 
                 Hs.293753 
                 NM_032515 
                 BOK 
                 0.0394 
                 0.0417 
                 0.9461 
               
               
                 37 
                 Hs.490366 
                 NM_004333 
                 BRAF 
                 −0.0590 
                 0.0616 
                 −0.9569 
               
               
                 38 
                 Hs.57973 
                 NM_014550 
                 CARD10 
                 −0.0769 
                 0.0960 
                 −0.8004 
               
               
                 39 
                 Hs.300355 
                 NM_032415 
                 CARD11 
                 −0.0676 
                 0.0818 
                 −0.8257 
               
               
                 40 
                 Hs.23248 
                 NM_021209 
                 CARD12 
                 −0.0497 
                 0.0446 
                 −1.1153 
               
               
                 41 
                 Hs.550529 
                 NM_024110 
                 CARD14 
                 0.0066 
                 −0.0080 
                 −0.8317 
               
               
                 42 
                 Hs.135201 
                 NM_022162 
                 CARD15 
                 0.0133 
                 0.0246 
                 0.5387 
               
               
                 43 
                 Hs.405153 
                 NM_006092 
                 CARD4 
                 0.0616 
                 0.0028 
                 21.9971 
               
               
                 44 
                 Hs.200242 
                 NM_032587 
                 CARD6 
                 0.2282 
                 −0.0227 
                 −10.0675 
               
               
                 45 
                 Hs.446146 
                 NM_014959 
                 CARD8 
                 0.1491 
                 −0.0048 
                 −30.9637 
               
               
                 46 
                 Hs.271815 
                 NM_022352 
                 CARD9 
                 0.0212 
                 0.0183 
                 1.1589 
               
               
                 47 
                 Hs.2490 
                 NM_033292 
                 CASP1 
                 0.0374 
                 0.1018 
                 0.3677 
               
               
                 48 
                 Hs.5353 
                 NM_001230 
                 CASP10 
                 0.0046 
                 0.1286 
                 0.0361 
               
               
                 49 
                 Hs.248226 
                 NM_012114 
                 CASP14 
                 0.0361 
                 0.0033 
                 10.8554 
               
               
                 50 
                 Hs.368982 
                 NM_001224 
                 CASP2 
                 0.1027 
                 0.0795 
                 1.2920 
               
               
                 51 
                 Hs.141125 
                 NM_004346 
                 CASP3 
                 0.1252 
                 −0.0479 
                 −2.6152 
               
               
                 52 
                 Hs.138378 
                 NM_001225 
                 CASP4 
                 0.0924 
                 −0.0382 
                 −2.4217 
               
               
                 53 
                 Hs.213327 
                 NM_004347 
                 CASP5 
                 0.0398 
                 −0.0941 
                 −0.4225 
               
               
                 54 
                 Hs.3280 
                 NM_001226 
                 CASP6 
                 −0.0096 
                 −0.0607 
                 0.1581 
               
               
                 55 
                 Hs.9216 
                 NM_001227 
                 CASP7 
                 0.0248 
                 0.0128 
                 1.9441 
               
               
                 56 
                 Hs.369736 
                 NM_001228 
                 CASP8 
                 0.0490 
                 0.0608 
                 0.8059 
               
               
                 57 
                 Hs.329502 
                 NM_001229 
                 CASP9 
                 0.2067 
                 0.0130 
                 15.8490 
               
               
                 58 
                 Hs.390736 
                 NM_003879 
                 CFLAR 
                 0.1444 
                 0.0028 
                 51.5632 
               
               
                 59 
                 Hs.249129 
                 NM_001279 
                 CIDEA 
                 0.0633 
                 −0.0014 
                 −45.1769 
               
               
                 60 
                 Hs.448590 
                 NM_014430 
                 CIDEB 
                 0.1517 
                 −0.0411 
                 −3.6957 
               
               
                 61 
                 Hs.38533 
                 NM_003805 
                 CRADD 
                 0.0583 
                 −0.0594 
                 −0.9809 
               
               
                 62 
                 Hs.380277 
                 NM_004938 
                 DAPK1 
                 0.0189 
                 −0.0321 
                 −0.5878 
               
               
                 63 
                 Hs.237886 
                 NM_014326 
                 DAPK2 
                 0.0388 
                 0.0217 
                 1.7854 
               
               
                 64 
                 Hs.484782 
                 NM_004401 
                 DFFA 
                 0.0961 
                 0.0776 
                 1.2373 
               
               
                 65 
                 Hs.133089 
                 NM_004402 
                 DFFB 
                 0.1020 
                 −0.0014 
                 −72.8507 
               
               
                 66 
                 Hs.86131 
                 NM_003824 
                 FADD 
                 0.2524 
                 −0.0384 
                 −6.5689 
               
               
                 67 
                 Hs.80409 
                 NM_001924 
                 GADD45A 
                 0.1941 
                 −0.0453 
                 −4.2895 
               
               
                 68 
                 Hs.248114 
                 NM_000514 
                 GDNF 
                 0.1362 
                 −0.0652 
                 −2.0878 
               
               
                 69 
                 Hs.87247 
                 NM_003806 
                 HRK 
                 −0.0560 
                 −0.0825 
                 0.6782 
               
               
                 70 
                 Hs.20573 
                 NM_000875 
                 IGF1R 
                 0.0086 
                 −0.0573 
                 −0.1502 
               
               
                 71 
                 Hs.36 
                 NM_000595 
                 LTA 
                 −0.0205 
                 −0.0166 
                 1.2349 
               
               
                 72 
                 Hs.1116 
                 NM_002342 
                 LTBR 
                 0.0699 
                 0.0950 
                 0.7360 
               
               
                 73 
                 Hs.532826 
                 NM_021960 
                 MCL1 
                 0.1424 
                 −0.0064 
                 −22.2920 
               
               
                 74 
                 Hs.513667 
                 NM_003946 
                 NOL3 
                 0.1030 
                 −0.0206 
                 −5.0084 
               
               
                 75 
                 Hs.499094 
                 NM_013258 
                 PYCARD 
                 0.0729 
                 −0.0544 
                 −1.3386 
               
               
                 76 
                 Hs.519842 
                 NM_003804 
                 RIPK1 
                 0.0099 
                 −0.0713 
                 −0.1395 
               
               
                 77 
                 Hs.103755 
                 NM_003821 
                 RIPK2 
                 0.1348 
                 0.0201 
                 6.6968 
               
               
                 78 
                 Hs.241570 
                 NM_000594 
                 TNF 
                 −0.0682 
                 −0.0844 
                 0.8087 
               
               
                 79 
                 Hs.401745 
                 NM_003844 
                 TNFRSF10A 
                 −0.0142 
                 −0.0403 
                 0.3538 
               
               
                 80 
                 Hs.521456 
                 NM_003842 
                 TNFRSF10B 
                 0.2047 
                 0.0582 
                 3.5170 
               
               
                 81 
                 Hs.119684 
                 NM_003841 
                 TNFRSF10C 
                 0.1375 
                 0.0130 
                 10.5406 
               
               
                 82 
                 Hs.213467 
                 NM_003840 
                 TNFRSF10D 
                 −0.0239 
                 −0.0592 
                 0.4031 
               
               
                 83 
                 Hs.81791 
                 NM_002546 
                 TNFRSF11B 
                 0.0331 
                 0.0049 
                 6.7580 
               
               
                 84 
                 Hs.355899 
                 NM_016639 
                 TNFRSF12A 
                 −0.0023 
                 −0.0119 
                 0.1948 
               
               
                 85 
                 Hs.512898 
                 NM_003820 
                 TNFRSF14 
                 −0.0212 
                 −0.0345 
                 0.6147 
               
               
                 86 
                 Hs.149168 
                 NM_018647 
                 TNFRSF19 
                 −0.0232 
                 −0.0870 
                 0.2665 
               
               
                 87 
                 Hs.279594 
                 NM_001065 
                 TNFRSF1A 
                 0.0199 
                 0.0256 
                 0.7750 
               
               
                 88 
                 Hs.256278 
                 NM_001066 
                 TNFRSF1B 
                 −0.0444 
                 0.0002 
                 −253.5583 
               
               
                 89 
                 Hs.443577 
                 NM_014452 
                 TNFRSF21 
                 0.0851 
                 −0.0418 
                 −2.0347 
               
               
                 90 
                 Hs.462529 
                 NM_003790 
                 TNFRSF25 
                 0.0437 
                 −0.0689 
                 −0.6347 
               
               
                 91 
                 Hs.472860 
                 NM_001250 
                 CD40 
                 0.0179 
                 −0.0883 
                 −0.2025 
               
               
                 92 
                 Hs.244139 
                 NM_152877 
                 FAS 
                 −0.0106 
                 −0.0886 
                 0.1197 
               
               
                 93 
                 Hs.434878 
                 NM_003823 
                 TNFRSF6B 
                 −0.0480 
                 −0.0846 
                 0.5675 
               
               
                 94 
                 Hs.355307 
                 NM_001242 
                 TNFRSF7 
                 −0.0411 
                 −0.1004 
                 0.4091 
               
               
                 95 
                 Hs.193418 
                 NM_001561 
                 TNFRSF9 
                 −0.1070 
                 −0.0552 
                 1.9372 
               
               
                 96 
                 Hs.478275 
                 NM_003810 
                 TNFSF10 
                 −0.0755 
                 −0.0221 
                 3.4105 
               
               
                 97 
                 Hs.129708 
                 NM_003807 
                 TNFSF14 
                 0.0358 
                 −0.0613 
                 −0.5839 
               
               
                 98 
                 Hs.248197 
                 NM_005092 
                 TNFSF18 
                 0.0354 
                 −0.0707 
                 −0.5012 
               
               
                 99 
                 Hs.652 
                 NM_000074 
                 CD40LG 
                 0.0457 
                 −0.0321 
                 −1.4230 
               
               
                 100 
                 Hs.2007 
                 NM_000639 
                 FASLG 
                 0.1474 
                 0.1443 
                 1.0213 
               
               
                 101 
                 Hs.501497 
                 NM_001252 
                 TNFSF7 
                 −0.0212 
                 −0.0605 
                 0.3505 
               
               
                 102 
                 Hs.494901 
                 NM_001244 
                 TNFSF8 
                 −0.0338 
                 0.0900 
                 −0.3755 
               
               
                 103 
                 Hs.1524 
                 NM_003811 
                 TNFSF9 
                 −0.0517 
                 0.0380 
                 −1.3603 
               
               
                 104 
                 Hs.408312 
                 NM_000546 
                 TP53 
                 0.0083 
                 0.1110 
                 0.0746 
               
               
                 105 
                 Hs.523968 
                 NM_005426 
                 TP53BP2 
                 0.1159 
                 0.0225 
                 5.1539 
               
               
                 106 
                 Hs.192132 
                 NM_005427 
                 TP73 
                 0.0520 
                 −0.0295 
                 −1.7631 
               
               
                 107 
                 Hs.137569 
                 NM_003722 
                 TP73L 
                 0.0855 
                 0.0485 
                 1.7624 
               
               
                 108 
                 Hs.460996 
                 NM_003789 
                 TRADD 
                 0.0480 
                 0.1010 
                 0.4755 
               
               
                 109 
                 Hs.531251 
                 NM_005658 
                 TRAF1 
                 −0.0133 
                 −0.0710 
                 0.1867 
               
               
                 110 
                 Hs.522506 
                 NM_021138 
                 TRAF2 
                 −0.0288 
                 −0.0355 
                 0.8110 
               
               
                 111 
                 Hs.510528 
                 NM_003300 
                 TRAF3 
                 −0.1259 
                 −0.0363 
                 3.4653 
               
               
                 112 
                 Hs.8375 
                 NM_004295 
                 TRAF4 
                 0.0149 
                 0.0771 
                 0.1933 
               
               
                 113 
                 Hs.523930 
                 NM_004619 
                 TRAF5 
                 0.0172 
                 −0.0298 
                 −0.5788 
               
               
                 114 
                   
                   
                 Blank 
                 0.0500 
                 −0.0547 
                 −0.9143 
               
               
                 115 
                 N/A 
                 L08752 
                 PUC18 
                 −0.0126 
                 −0.0463 
                 0.2719 
               
               
                 116 
                   
                   
                 Blank 
                 −0.0636 
                 −0.0064 
                 9.9536 
               
               
                 117 
                   
                   
                 Blank 
                 0.0136 
                 0.0611 
                 0.2223 
               
               
                 118 
                 N/A 
                 N/A 
                 AS1R2 
                 −0.0129 
                 0.1307 
                 −0.0989 
               
               
                 119 
                 N/A 
                 N/A 
                 AS1R1 
                 0.2653 
                 0.1974 
                 1.3443 
               
               
                 120 
                 N/A 
                 N/A 
                 AS1 
                 0.9584 
                 0.8912 
                 1.0754 
               
               
                 121 
                 Hs.544577 
                 NM_002046 
                 GAPD 
                 0.7006 
                 1.0997 
                 0.6371 
               
               
                 122 
                 Hs.534255 
                 NM_004048 
                 B2M 
                 0.2839 
                 0.1086 
                 2.6134 
               
               
                 123 
                 Hs.509736 
                 NM_007355 
                 HSPCB 
                 0.9918 
                 0.8395 
                 1.1815 
               
               
                 124 
                 Hs.509736 
                 NM_007355 
                 HSPCB 
                 0.8686 
                 0.8668 
                 1.0021 
               
               
                 125 
                 Hs.520640 
                 NM_001101 
                 ACTB 
                 1.5778 
                 1.2042 
                 1.3103 
               
               
                 126 
                 Hs.520640 
                 NM_001101 
                 ACTB 
                 1.3890 
                 1.5086 
                 0.9208 
               
               
                 127 
                 N/A 
                 N/A 
                 BAS2C 
                 2.1066 
                 1.7055 
                 1.2351 
               
               
                 128 
                 N/A 
                 N/A 
                 BAS2C 
                 2.0996 
                 1.6617 
                 1.2635 
               
               
                   
               
               
                 * As default, Group 1 is used as control group. 
               
             
          
         
       
     
         [0000]    
       
         
               
               
               
             
               
               
               
               
             
               
               
               
               
               
               
               
             
           
               
                   
                   
               
             
             
               
                   
                 Project 
                 Pao Cell Cycle 100 ug/ml 24 hr LNCaP 
               
               
                   
                 Catalog # 
                 Oligo GEArray ® Human Cell Cycle Microarray 
               
               
                   
                 Density 
                 Average 
               
               
                   
                 Clover 
                 Clover Off 
               
               
                   
                 Total Arrays 
                 2 
               
               
                   
                 Total Groups 
                 2 
               
               
                   
                 Background 
                 Empty spots: positions 117, 114, 116 
               
               
                   
                 Normalization 
                 Selected genes: positions 125 
               
             
          
           
               
                   
                 Array Name 
                 T cell cycle light.tif 
                 T cell cycle light.tif 
               
               
                   
                 Assigned Group 
                 Group 1 
                 Group 2 
               
               
                   
                   
               
             
          
           
               
                 Position 
                 UniGene 
                 RefSeq # 
                 Symbol 
                 Group 2 
                 Group 1 
                 Group 2/Group 1 
               
               
                   
               
               
                 1 
                 Hs.546292 
                 NM_002954 
                 RPS27A 
                 0.992 
                 0.956 
                 1.039 
               
               
                 2 
                 Hs.431048 
                 NM_005157 
                 ABL1 
                 0.048 
                 0.078 
                 0.622 
               
               
                 3 
                 Hs.533262 
                 NM_013366 
                 ANAPC2 
                 0.010 
                 0.009 
                 1.056 
               
               
                 4 
                 Hs.152173 
                 NM_013367 
                 
                   ANAPC4 
                 
                 
                   0.049 
                 
                 
                   0.106 
                 
                 
                   0.467 
                 
               
               
                 5 
                 Hs.7101 
                 NM_016237 
                 
                   ANAPC5 
                 
                 
                   0.118 
                 
                 
                   0.281 
                 
                 
                   0.422 
                 
               
               
                 6 
                 Hs.194695 
                 NM_004675 
                 ARHI 
                 0.002 
                 −0.000 
                 −6.380 
               
               
                 7 
                 Hs.435561 
                 NM_000051 
                 ATM 
                 0.029 
                 0.042 
                 0.682 
               
               
                 8 
                 Hs.271791 
                 NM_001184 
                 
                   ATR 
                 
                 
                   0.015 
                 
                 
                   0.031 
                 
                 
                   0.493 
                 
               
               
                 9 
                 Hs.159428 
                 NM_004324 
                 BAX 
                 0.007 
                 0.004 
                 1.659 
               
               
                 10 
                 Hs.370292 
                 NM_016567 
                 BCCIP 
                 0.012 
                 0.020 
                 0.605 
               
               
                 11 
                 Hs.150749 
                 NM_000633 
                 BCL2 
                 0.004 
                 0.001 
                 6.347 
               
               
                 12 
                 Hs.514527 
                 NM_001168 
                 
                   BIRC5 
                 
                 
                   0.224 
                 
                 
                   0.806 
                 
                 
                   0.278 
                 
               
               
                 13 
                 Hs.194143 
                 NM_007294 
                 
                   BRCA1 
                 
                 
                   0.006 
                 
                 
                   0.030 
                 
                 
                   0.182 
                 
               
               
                 14 
                 Hs.34012 
                 NM_000059 
                 BRCA2 
                 0.003 
                 0.000 
                 35.636 
               
               
                 15 
                 Hs.417050 
                 NM_003914 
                 CCNA1 
                 0.002 
                 0.002 
                 1.177 
               
               
                 16 
                 Hs.85137 
                 NM_001237 
                 
                   CCNA2 
                 
                 
                   0.007 
                 
                 
                   0.014 
                 
                 
                   0.476 
                 
               
               
                 17 
                 Hs.23960 
                 NM_031966 
                 
                   CCNB1 
                 
                 
                   0.048 
                 
                 
                   0.128 
                 
                 
                   0.371 
                 
               
               
                 18 
                 Hs.194698 
                 NM_004701 
                 CCNB2 
                 0.008 
                 0.004 
                 1.945 
               
               
                 19 
                 Hs.430646 
                 NM_005190 
                 
                   CCNC 
                 
                 
                   0.013 
                 
                 
                   0.004 
                 
                 
                   3.493 
                 
               
               
                 20 
                 Hs.523852 
                 NM_053056 
                 CCND1 
                 0.007 
                 0.000 
                 16.573 
               
               
                 21 
                 Hs.376071 
                 NM_001759 
                 CCND2 
                 0.000 
                 −0.000 
                 −2.759 
               
               
                 22 
                 Hs.534307 
                 NM_001760 
                 
                   CCND3 
                 
                 
                   0.337 
                 
                 
                   0.805 
                 
                 
                   0.419 
                 
               
               
                 23 
                 Hs.244723 
                 NM_001238 
                 CCNE1 
                 0.042 
                 0.080 
                 0.525 
               
               
                 24 
                 Hs.408658 
                 NM_004702 
                 CCNE2 
                 0.007 
                 0.009 
                 0.774 
               
               
                 25 
                 Hs.1973 
                 NM_001761 
                 CCNF 
                 0.002 
                 0.001 
                 3.677 
               
               
                 26 
                 Hs.79101 
                 NM_004060 
                 CCNG1 
                 0.014 
                 0.013 
                 1.033 
               
               
                 27 
                 Hs.13291 
                 NM_004354 
                 CCNG2 
                 0.057 
                 0.098 
                 0.579 
               
               
                 28 
                 Hs.146607 
                 NM_001239 
                 
                   CCNH 
                 
                 
                   0.244 
                 
                 
                   0.691 
                 
                 
                   0.353 
                 
               
               
                 29 
                 Hs.279906 
                 NM_001240 
                 CCNT1 
                 0.004 
                 −0.000 
                 −14.118 
               
               
                 30 
                 Hs.292754 
                 NM_001241 
                 CCNT2 
                 0.015 
                 0.013 
                 1.161 
               
               
                 31 
                 Hs.374127 
                 NM_003903 
                 CDC16 
                 0.083 
                 0.140 
                 0.594 
               
               
                 32 
                 Hs.334562 
                 NM_001786 
                 CDC2 
                 0.006 
                 0.004 
                 1.365 
               
               
                 33 
                 Hs.524947 
                 NM_001255 
                 
                   CDC20 
                 
                 
                   0.062 
                 
                 
                   0.362 
                 
                 
                   0.171 
                 
               
               
                 34 
                 Hs.1634 
                 NM_001789 
                 CDC25A 
                 0.001 
                 −0.003 
                 −0.307 
               
               
                 35 
                 Hs.656 
                 NM_001790 
                 
                   CDC25C 
                 
                 
                   0.008 
                 
                 
                   0.021 
                 
                 
                   0.366 
                 
               
               
                 36 
                 Hs.514997 
                 NM_004359 
                 CDC34 
                 0.031 
                 0.060 
                 0.516 
               
               
                 37 
                 Hs.160958 
                 NM_007065 
                 CDC37 
                 0.020 
                 0.015 
                 1.333 
               
               
                 38 
                 Hs.474217 
                 NM_003504 
                 CDC45L 
                 0.003 
                 0.000 
                 37.346 
               
               
                 39 
                 Hs.405958 
                 NM_001254 
                 CDC6 
                 0.007 
                 0.009 
                 0.793 
               
               
                 40 
                 Hs.533573 
                 NM_003503 
                 CDC7 
                 0.001 
                 0.002 
                 0.569 
               
               
                 41 
                 Hs.19192 
                 NM_001798 
                 
                   CDK2 
                 
                 
                   0.045 
                 
                 
                   0.137 
                 
                 
                   0.330 
                 
               
               
                 42 
                 Hs.95577 
                 NM_000075 
                 CDK4 
                 0.770 
                 0.957 
                 0.805 
               
               
                 43 
                 Hs.500015 
                 NM_003885 
                 CDK5R1 
                 0.004 
                 −0.002 
                 −2.473 
               
               
                 44 
                 Hs.158460 
                 NM_003936 
                 CDK5R2 
                 0.002 
                 −0.000 
                 −4.346 
               
               
                 45 
                 Hs.435952 
                 NM_016408 
                 CDK5RAP1 
                 0.006 
                 0.010 
                 0.576 
               
               
                 46 
                 Hs.20157 
                 NM_176096 
                 CDK5RAP3 
                 0.001 
                 0.000 
                 7.638 
               
               
                 47 
                 Hs.119882 
                 NM_001259 
                 CDK6 
                 0.003 
                 0.001 
                 5.750 
               
               
                 48 
                 Hs.184298 
                 NM_001799 
                 CDK7 
                 0.037 
                 0.036 
                 1.036 
               
               
                 49 
                 Hs.382306 
                 NM_001260 
                 
                   CDK8 
                 
                 
                   0.022 
                 
                 
                   0.003 
                 
                 
                   8.117 
                 
               
               
                 50 
                 Hs.370771 
                 NM_000389 
                 CDKN1A 
                 0.912 
                 0.865 
                 1.054 
               
               
                 51 
                 Hs.238990 
                 NM_004064 
                 CDKN1B 
                 0.041 
                 0.043 
                 0.956 
               
               
                 52 
                 Hs.106070 
                 NM_000076 
                 CDKN1C 
                 0.001 
                 −0.002 
                 −0.481 
               
               
                 53 
                 Hs.421349 
                 NM_058195 
                 
                   CDKN2A 
                 
                 
                   0.012 
                 
                 
                   0.026 
                 
                 
                   0.452 
                 
               
               
                 54 
                 Hs.72901 
                 NM_004936 
                 CDKN2B 
                 0.006 
                 0.005 
                 1.094 
               
               
                 55 
                 Hs.525324 
                 NM_078626 
                 
                   CDKN2C 
                 
                 
                   0.021 
                 
                 
                   0.068 
                 
                 
                   0.312 
                 
               
               
                 56 
                 Hs.435051 
                 NM_001800 
                 
                   CDKN2D 
                 
                 
                   0.019 
                 
                 
                   0.045 
                 
                 
                   0.424 
                 
               
               
                 57 
                 Hs.84113 
                 NM_005192 
                 CDKN3 
                 0.025 
                 0.018 
                 1.406 
               
               
                 58 
                 Hs.24529 
                 NM_001274 
                 
                   CHEK1 
                 
                 
                   0.007 
                 
                 
                   0.002 
                 
                 
                   3.101 
                 
               
               
                 59 
                 Hs.291363 
                 NM_007194 
                 CHEK2 
                 0.002 
                 0.003 
                 0.593 
               
               
                 60 
                 Hs.374378 
                 NM_001826 
                 
                   CKS1B 
                 
                 
                   0.045 
                 
                 
                   0.123 
                 
                 
                   0.369 
                 
               
               
                 61 
                 Hs.83758 
                 NM_001827 
                 
                   CKS2 
                 
                 
                   0.330 
                 
                 
                   0.679 
                 
                 
                   0.486 
                 
               
               
                 62 
                 Hs.146806 
                 NM_003592 
                 CUL1 
                 0.399 
                 0.538 
                 0.743 
               
               
                 63 
                 Hs.82919 
                 NM_003591 
                 CUL2 
                 0.010 
                 0.015 
                 0.662 
               
               
                 64 
                 Hs.372286 
                 NM_003590 
                 CUL3 
                 0.008 
                 0.000 
                 20.679 
               
               
                 65 
                 Hs.339735 
                 NM_003589 
                 
                   CUL4A 
                 
                 
                   0.043 
                 
                 
                   0.022 
                 
                 
                   1.923 
                 
               
               
                 66 
                 Hs.440320 
                 NM_003478 
                 
                   CUL5 
                 
                 
                   0.016 
                 
                 
                   0.002 
                 
                 
                   9.800 
                 
               
               
                 67 
                 Hs.443960 
                 NM_004399 
                 DDX11 
                 0.008 
                 0.015 
                 0.525 
               
               
                 68 
                 Hs.211463 
                 NM_004945 
                 DNM2 
                 0.003 
                 0.002 
                 1.762 
               
               
                 69 
                 Hs.96055 
                 NM_005225 
                 
                   E2F1 
                 
                 
                   0.100 
                 
                 
                   0.278 
                 
                 
                   0.359 
                 
               
               
                 70 
                 Hs.194333 
                 NM_004091 
                 
                   E2F2 
                 
                 
                   0.015 
                 
                 
                   0.045 
                 
                 
                   0.322 
                 
               
               
                 71 
                 Hs.269408 
                 NM_001949 
                 E2F3 
                 0.004 
                 0.001 
                 5.082 
               
               
                 72 
                 Hs.108371 
                 NM_001950 
                 
                   E2F4 
                 
                 
                   0.008 
                 
                 
                   0.004 
                 
                 
                   2.180 
                 
               
               
                 73 
                 Hs.445758 
                 NM_001951 
                 E2F5 
                 0.008 
                 0.000 
                 98.497 
               
               
                 74 
                 Hs.135465 
                 NM_001952 
                 E2F6 
                 0.001 
                 −0.001 
                 −0.743 
               
               
                 75 
                 Hs.80409 
                 NM_001924 
                 
                   GADD45A 
                 
                 
                   0.075 
                 
                 
                   0.032 
                 
                 
                   2.310 
                 
               
               
                 76 
                 Hs.523510 
                 NM_005316 
                 GTF2H1 
                 0.024 
                 0.013 
                 1.858 
               
               
                 77 
                 Hs.386189 
                 NM_016426 
                 GTSE1 
                 0.006 
                 0.007 
                 0.828 
               
               
                 78 
                 Hs.26663 
                 NM_016323 
                 HERC5 
                 0.001 
                 −0.001 
                 −1.466 
               
               
                 79 
                 Hs.152983 
                 NM_004507 
                 HUS1 
                 0.004 
                 −0.000 
                 −28.200 
               
               
                 80 
                 Hs.300559 
                 NM_014708 
                 KNTC1 
                 0.005 
                 0.004 
                 1.207 
               
               
                 81 
                 Hs.252712 
                 NM_002266 
                 KPNA2 
                 0.180 
                 0.346 
                 0.520 
               
               
                 82 
                 Hs.533185 
                 NM_002358 
                 MAD2L1 
                 0.026 
                 0.035 
                 0.737 
               
               
                 83 
                 Hs.19400 
                 NM_006341 
                 MAD2L2 
                 0.118 
                 0.154 
                 0.767 
               
               
                 84 
                 Hs.477481 
                 NM_004526 
                 MCM2 
                 0.377 
                 0.563 
                 0.669 
               
               
                 85 
                 Hs.179565 
                 NM_002388 
                 MCM3 
                 0.007 
                 0.007 
                 1.025 
               
               
                 86 
                 Hs.460184 
                 NM_005914 
                 MCM4 
                 0.003 
                 0.002 
                 2.108 
               
               
                 87 
                 Hs.517582 
                 NM_006739 
                 MCM5 
                 0.009 
                 0.016 
                 0.600 
               
               
                 88 
                 Hs.444118 
                 NM_005915 
                 MCM6 
                 0.010 
                 0.008 
                 1.202 
               
               
                 89 
                 Hs.438720 
                 NM_005916 
                 
                   MCM7 
                 
                 
                   0.142 
                 
                 
                   0.345 
                 
                 
                   0.411 
                 
               
               
                 90 
                 Hs.80976 
                 NM_002417 
                 
                   MKI67 
                 
                 
                   0.006 
                 
                 
                   0.032 
                 
                 
                   0.194 
                 
               
               
                 91 
                 Hs.509523 
                 NM_002431 
                 MNAT1 
                 0.003 
                 0.002 
                 1.406 
               
               
                 92 
                 Hs.20555 
                 NM_005590 
                 MRE11A 
                 0.001 
                 0.004 
                 0.350 
               
               
                 93 
                 Hs.492208 
                 NM_002485 
                 NBS1 
                 0.003 
                 0.002 
                 1.834 
               
               
                 94 
                 Hs.147433 
                 NM_182649 
                 PCNA 
                 0.514 
                 0.744 
                 0.691 
               
               
                 95 
                 Hs.77783 
                 NM_182687 
                 
                   PKMYT1 
                 
                 
                   0.029 
                 
                 
                   0.144 
                 
                 
                   0.201 
                 
               
               
                 96 
                 Hs.531879 
                 NM_002853 
                 RAD1 
                 0.017 
                 0.011 
                 1.554 
               
               
                 97 
                 Hs.16184 
                 NM_002873 
                 RAD17 
                 0.006 
                 0.001 
                 9.384 
               
               
                 98 
                 Hs.242635 
                 NM_005732 
                 RAD50 
                 0.005 
                 0.001 
                 4.031 
               
               
                 99 
                 Hs.446554 
                 NM_002875 
                 RAD51 
                 0.003 
                 −0.001 
                 −2.643 
               
               
                 100 
                 Hs.240457 
                 NM_004584 
                 
                   RAD9A 
                 
                 
                   0.010 
                 
                 
                   0.020 
                 
                 
                   0.489 
                 
               
               
                 101 
                 Hs.408528 
                 NM_000321 
                 
                   RB1 
                 
                 
                   0.032 
                 
                 
                   0.010 
                 
                 
                   3.188 
                 
               
               
                 102 
                 Hs.546282 
                 NM_002894 
                 RBBP8 
                 0.010 
                 0.011 
                 0.890 
               
               
                 103 
                 Hs.207745 
                 NM_002895 
                 RBL1 
                 0.008 
                 0.005 
                 1.414 
               
               
                 104 
                 Hs.513609 
                 NM_005611 
                 RBL2 
                 0.023 
                 0.018 
                 1.307 
               
               
                 105 
                 Hs.507866 
                 NM_014059 
                 RGC32 
                 0.005 
                 −0.003 
                 −1.854 
               
               
                 106 
                 Hs.487540 
                 NM_002947 
                 RPA3 
                 0.002 
                 0.000 
                 28.121 
               
               
                 107 
                 Hs.269898 
                 NM_013376 
                 SERTAD1 
                 0.009 
                 0.006 
                 1.665 
               
               
                 108 
                 Hs.23348 
                 NM_005983 
                 SKP2 
                 −0.000 
                 −0.001 
                 0.039 
               
               
                 109 
                 Hs.81424 
                 NM_003352 
                 SUMO1 
                 0.027 
                 0.027 
                 1.012 
               
               
                 110 
                 Hs.79353 
                 NM_007111 
                 TFDP1 
                 0.035 
                 0.038 
                 0.901 
               
               
                 111 
                 Hs.379018 
                 NM_006286 
                 TFDP2 
                 0.008 
                 0.006 
                 1.346 
               
               
                 112 
                 Hs.408312 
                 NM_000546 
                 TP53 
                 0.016 
                 0.018 
                 0.914 
               
               
                 113 
                 Hs.406693 
                 NM_003334 
                 UBE1 
                 0.061 
                 0.044 
                 1.381 
               
               
                 114 
                   
                   
                 Blank 
                 −0.000 
                 0.001 
                 −0.240 
               
               
                 115 
                 N/A 
                 L08752 
                 PUC18 
                 −0.001 
                 −0.001 
                 0.911 
               
               
                 116 
                   
                   
                 Blank 
                 −0.000 
                 −0.001 
                 0.190 
               
               
                 117 
                   
                   
                 Blank 
                 0.000 
                 0.001 
                 0.555 
               
               
                 118 
                 N/A 
                 N/A 
                 AS1R2 
                 0.006 
                 0.002 
                 3.958 
               
               
                 119 
                 N/A 
                 N/A 
                 AS1R1 
                 −0.000 
                 0.004 
                 −0.110 
               
               
                 120 
                 N/A 
                 N/A 
                 AS1 
                 0.003 
                 0.004 
                 0.627 
               
               
                 121 
                 Hs.544577 
                 NM_002046 
                 GAPD 
                 0.911 
                 0.990 
                 0.920 
               
               
                 122 
                 Hs.534255 
                 NM_004048 
                 B2M 
                 0.333 
                 0.487 
                 0.684 
               
               
                 123 
                 Hs.509736 
                 NM_007355 
                 HSPCB 
                 0.981 
                 0.917 
                 1.070 
               
               
                 124 
                 Hs.509736 
                 NM_007355 
                 HSPCB 
                 0.987 
                 0.964 
                 1.024 
               
               
                 125 
                 Hs.520640 
                 NM_001101 
                 ACTB 
                 1.000 
                 1.000 
                 1.000 
               
               
                 126 
                 Hs.520640 
                 NM_001101 
                 ACTB 
                 0.980 
                 0.967 
                 1.013 
               
               
                 127 
                 N/A 
                 N/A 
                 BAS2C 
                 0.032 
                 0.093 
                 0.347 
               
               
                 128 
                 N/A 
                 N/A 
                 BAS2C 
                 0.669 
                 0.841 
                 0.795 
               
               
                   
               
               
                 * As default, Group 1 is used as control group. 
               
             
          
         
       
     
         [0000]    
       
         
               
               
               
             
               
               
               
               
             
               
               
               
               
               
               
               
             
           
               
                   
                   
               
             
             
               
                   
                 Project 
                 Rauwolfia Cell Cycle 500 ug/ml 24 hr LNCaP 
               
               
                   
                 Catalog # 
                 Oligo GEArray ® Human Cell Cycle Microarray 
               
               
                   
                 Density 
                 Average 
               
               
                   
                 Clover 
                 Clover Off 
               
               
                   
                 Total Arrays 
                 2 
               
               
                   
                 Total Groups 
                 2 
               
               
                   
                 Background 
                 Empty spots: positions 117, 114, 116 
               
               
                   
                 Normalization 
                 Selected genes: positions 1, 121, 122, 123, 124, 
               
               
                   
                   
                 125, 126 
               
               
                   
                 Array Name 
                 Rauwolfia Rx Cell Cycle.tif 
               
             
          
           
               
                   
                 Assigned Group 
                 Group 1 
                 Group 2 
               
               
                   
                   
               
             
          
           
               
                 Position 
                 UniGene 
                 RefSeq # 
                 Symbol 
                 Group 2 
                 Group 1 
                 Group 2/Group 1 
               
               
                   
               
               
                 1 
                 Hs.546292 
                 NM_002954 
                 RPS27A 
                 0.9708 
                 0.7410 
                 1.3102 
               
               
                 2 
                 Hs.431048 
                 NM_005157 
                 ABL1 
                 0.0617 
                 0.0930 
                 0.6641 
               
               
                 3 
                 Hs.533262 
                 NM_013366 
                 ANAPC2 
                 0.3128 
                 0.3405 
                 0.9186 
               
               
                 4 
                 Hs.152173 
                 NM_013367 
                 ANAPC4 
                 0.0785 
                 0.0452 
                 1.7375 
               
               
                 5 
                 Hs.7101 
                 NM_016237 
                 ANAPC5 
                 0.0074 
                 −0.0339 
                 −0.2169 
               
               
                 6 
                 Hs.194695 
                 NM_004675 
                 ARHI 
                 0.0092 
                 −0.0952 
                 −0.0969 
               
               
                 7 
                 Hs.435561 
                 NM_000051 
                 ATM 
                 0.5787 
                 0.5695 
                 1.0162 
               
               
                 8 
                 Hs.271791 
                 NM_001184 
                 ATR 
                 0.1767 
                 −0.0030 
                 −57.9743 
               
               
                 9 
                 Hs.159428 
                 NM_004324 
                 BAX 
                 0.0493 
                 0.0381 
                 1.2941 
               
               
                 10 
                 Hs.370292 
                 NM_016567 
                 
                   BCCIP 
                 
                 
                   0.1229 
                 
                 
                   0.0324 
                 
                 
                   3.7965 
                 
               
               
                 11 
                 Hs.150749 
                 NM_000633 
                 
                   BCL2 
                 
                 
                   0.1167 
                 
                 
                   0.0552 
                 
                 
                   2.1130 
                 
               
               
                 12 
                 Hs.514527 
                 NM_001168 
                 
                   BIRC5 
                 
                 
                   0.0996 
                 
                 
                   0.1983 
                 
                 
                   0.5024 
                 
               
               
                 13 
                 Hs.194143 
                 NM_007294 
                 BRCA1 
                 0.1245 
                 0.1423 
                 0.8747 
               
               
                 14 
                 Hs.34012 
                 NM_000059 
                 BRCA2 
                 0.0328 
                 −0.0721 
                 −0.4555 
               
               
                 15 
                 Hs.417050 
                 NM_003914 
                 CCNA1 
                 0.2733 
                 −0.0522 
                 −5.2368 
               
               
                 16 
                 Hs.85137 
                 NM_001237 
                 CCNA2 
                 0.2059 
                 −0.0202 
                 −10.1973 
               
               
                 17 
                 Hs.23960 
                 NM_031966 
                 CCNB1 
                 0.0664 
                 0.0520 
                 1.2757 
               
               
                 18 
                 Hs.194698 
                 NM_004701 
                 
                   CCNB2 
                 
                 
                   0.1124 
                 
                 
                   0.0495 
                 
                 
                   2.2690 
                 
               
               
                 19 
                 Hs.430646 
                 NM_005190 
                 CCNC 
                 0.0888 
                 0.0834 
                 1.0648 
               
               
                 20 
                 Hs.523852 
                 NM_053056 
                 
                   CCND1 
                 
                 
                   0.0136 
                 
                 
                   0.6031 
                 
                 
                   0.0225 
                 
               
               
                 21 
                 Hs.376071 
                 NM_001759 
                 CCND2 
                 0.0042 
                 −0.0152 
                 −0.2801 
               
               
                 22 
                 Hs.534307 
                 NM_001760 
                 CCND3 
                 0.1037 
                 0.1890 
                 0.5487 
               
               
                 23 
                 Hs.244723 
                 NM_001238 
                 CCNE1 
                 0.1121 
                 −0.0053 
                 −21.0110 
               
               
                 24 
                 Hs.408658 
                 NM_004702 
                 CCNE2 
                 0.0241 
                 −0.0277 
                 −0.8701 
               
               
                 25 
                 Hs.1973 
                 NM_001761 
                 CCNF 
                 0.0869 
                 0.0509 
                 1.7073 
               
               
                 26 
                 Hs.79101 
                 NM_004060 
                 CCNG1 
                 0.1559 
                 0.1108 
                 1.4070 
               
               
                 27 
                 Hs.13291 
                 NM_004354 
                 CCNG2 
                 0.0962 
                 0.1149 
                 0.8374 
               
               
                 28 
                 Hs.146607 
                 NM_001239 
                 
                   CCNH 
                 
                 
                   0.0272 
                 
                 
                   0.1042 
                 
                 
                   0.2615 
                 
               
               
                 29 
                 Hs.279906 
                 NM_001240 
                 CCNT1 
                 −0.0501 
                 −0.0081 
                 6.2066 
               
               
                 30 
                 Hs.292754 
                 NM_001241 
                 CCNT2 
                 0.0285 
                 −0.0394 
                 −0.7230 
               
               
                 31 
                 Hs.374127 
                 NM_003903 
                 CDC16 
                 0.1177 
                 −0.0072 
                 −16.4273 
               
               
                 32 
                 Hs.334562 
                 NM_001786 
                 CDC2 
                 −0.0191 
                 −0.0428 
                 0.4450 
               
               
                 33 
                 Hs.524947 
                 NM_001255 
                 CDC20 
                 0.1136 
                 0.0948 
                 1.1987 
               
               
                 34 
                 Hs.1634 
                 NM_001789 
                 CDC25A 
                 0.0962 
                 0.1126 
                 0.8544 
               
               
                 35 
                 Hs.656 
                 NM_001790 
                 CDC25C 
                 0.0686 
                 0.0795 
                 0.8628 
               
               
                 36 
                 Hs.514997 
                 NM_004359 
                 CDC34 
                 0.0282 
                 −0.0332 
                 −0.8480 
               
               
                 37 
                 Hs.160958 
                 NM_007065 
                 CDC37 
                 −0.0100 
                 −0.0202 
                 0.4976 
               
               
                 38 
                 Hs.474217 
                 NM_003504 
                 CDC45L 
                 −0.0501 
                 −0.1244 
                 0.4029 
               
               
                 39 
                 Hs.405958 
                 NM_001254 
                 CDC6 
                 0.0204 
                 −0.1619 
                 −0.1260 
               
               
                 40 
                 Hs.533573 
                 NM_003503 
                 CDC7 
                 −0.0085 
                 0.0013 
                 −6.5566 
               
               
                 41 
                 Hs.19192 
                 NM_001798 
                 CDK2 
                 0.2109 
                 0.2500 
                 0.8435 
               
               
                 42 
                 Hs.95577 
                 NM_000075 
                 CDK4 
                 0.4482 
                 0.7110 
                 0.6304 
               
               
                 43 
                 Hs.500015 
                 NM_003885 
                 CDK5R1 
                 0.1462 
                 0.1259 
                 1.1618 
               
               
                 44 
                 Hs.158460 
                 NM_003936 
                 CDK5R2 
                 0.0869 
                 0.0280 
                 3.0991 
               
               
                 45 
                 Hs.435952 
                 NM_016408 
                 CDK5RAP1 
                 0.0738 
                 −0.0846 
                 −0.8724 
               
               
                 46 
                 Hs.20157 
                 NM_176096 
                 CDK5RAP3 
                 0.0742 
                 0.0242 
                 3.0702 
               
               
                 47 
                 Hs.119882 
                 NM_001259 
                 CDK6 
                 −0.0271 
                 0.0811 
                 −0.3347 
               
               
                 48 
                 Hs.184298 
                 NM_001799 
                 CDK7 
                 0.0055 
                 0.0248 
                 0.2210 
               
               
                 49 
                 Hs.382306 
                 NM_001260 
                 CDK8 
                 0.1003 
                 0.1291 
                 0.7767 
               
               
                 50 
                 Hs.370771 
                 NM_000389 
                 
                   CDKN1A 
                 
                 
                   2.1099 
                 
                 
                   0.4946 
                 
                 
                   4.2662 
                 
               
               
                 51 
                 Hs.238990 
                 NM_004064 
                 CDKN1B 
                 0.1525 
                 0.1284 
                 1.1875 
               
               
                 52 
                 Hs.106070 
                 NM_000076 
                 CDKN1C 
                 0.0493 
                 0.0280 
                 1.7582 
               
               
                 53 
                 Hs.421349 
                 NM_058195 
                 CDKN2A 
                 0.0307 
                 0.0098 
                 3.1434 
               
               
                 54 
                 Hs.72901 
                 NM_004936 
                 CDKN2B 
                 0.0515 
                 0.0575 
                 0.8948 
               
               
                 55 
                 Hs.525324 
                 NM_078626 
                 CDKN2C 
                 0.1229 
                 0.0891 
                 1.3802 
               
               
                 56 
                 Hs.435051 
                 NM_001800 
                 CDKN2D 
                 0.0639 
                 0.0626 
                 1.0215 
               
               
                 57 
                 Hs.84113 
                 NM_005192 
                 CDKN3 
                 0.0667 
                 0.0790 
                 0.8442 
               
               
                 58 
                 Hs.24529 
                 NM_001274 
                 CHEK1 
                 0.0863 
                 0.1007 
                 0.8565 
               
               
                 59 
                 Hs.291363 
                 NM_007194 
                 CHEK2 
                 0.1260 
                 0.0820 
                 1.5374 
               
               
                 60 
                 Hs.374378 
                 NM_001826 
                 CKS1B 
                 0.0978 
                 0.0578 
                 1.6929 
               
               
                 61 
                 Hs.83758 
                 NM_001827 
                 CKS2 
                 0.1111 
                 0.1339 
                 0.8301 
               
               
                 62 
                 Hs.146806 
                 NM_003592 
                 CUL1 
                 0.1422 
                 0.1199 
                 1.1857 
               
               
                 63 
                 Hs.82919 
                 NM_003591 
                 
                   CUL2 
                 
                 
                   0.1726 
                 
                 
                   0.0644 
                 
                 
                   2.6816 
                 
               
               
                 64 
                 Hs.372286 
                 NM_003590 
                 
                   CUL3 
                 
                 
                   0.1447 
                 
                 
                   0.0637 
                 
                 
                   2.2715 
                 
               
               
                 65 
                 Hs.339735 
                 NM_003589 
                 CUL4A 
                 0.1267 
                 0.1076 
                 1.1774 
               
               
                 66 
                 Hs.440320 
                 NM_003478 
                 CUL5 
                 0.4051 
                 0.4319 
                 0.9378 
               
               
                 67 
                 Hs.443960 
                 NM_004399 
                 DDX11 
                 0.1369 
                 0.1250 
                 1.0957 
               
               
                 68 
                 Hs.211463 
                 NM_004945 
                 
                   DNM2 
                 
                 
                   0.1456 
                 
                 
                   0.0084 
                 
                 
                   17.3744 
                 
               
               
                 69 
                 Hs.96055 
                 NM_005225 
                 
                   E2F1 
                 
                 
                   0.1357 
                 
                 
                   0.3227 
                 
                 
                   0.4205 
                 
               
               
                 70 
                 Hs.194333 
                 NM_004091 
                 E2F2 
                 0.0244 
                 0.0223 
                 1.0949 
               
               
                 71 
                 Hs.269408 
                 NM_001949 
                 
                   E2F3 
                 
                 
                   0.1363 
                 
                 
                   0.0443 
                 
                 
                   3.0789 
                 
               
               
                 72 
                 Hs.108371 
                 NM_001950 
                 
                   E2F4 
                 
                 
                   0.1388 
                 
                 
                   0.0482 
                 
                 
                   2.8821 
                 
               
               
                 73 
                 Hs.445758 
                 NM_001951 
                 E2F5 
                 −0.0265 
                 0.0454 
                 −0.5839 
               
               
                 74 
                 Hs.135465 
                 NM_001952 
                 E2F6 
                 0.0316 
                 0.0079 
                 3.9864 
               
               
                 75 
                 Hs.80409 
                 NM_001924 
                 
                   GADD45A 
                 
                 
                   0.1465 
                 
                 
                   0.0475 
                 
                 
                   3.0873 
                 
               
               
                 76 
                 Hs.523510 
                 NM_005316 
                 GTF2H1 
                 0.0515 
                 0.0123 
                 4.1961 
               
               
                 77 
                 Hs.386189 
                 NM_016426 
                 GTSE1 
                 0.0878 
                 0.0397 
                 2.2125 
               
               
                 78 
                 Hs.26663 
                 NM_016323 
                 HERC5 
                 0.0207 
                 0.0461 
                 0.4494 
               
               
                 79 
                 Hs.152983 
                 NM_004507 
                 HUS1 
                 0.0947 
                 0.0226 
                 4.1973 
               
               
                 80 
                 Hs.300559 
                 NM_014708 
                 KNTC1 
                 0.0748 
                 0.0148 
                 5.0589 
               
               
                 81 
                 Hs.252712 
                 NM_002266 
                 KPNA2 
                 0.0014 
                 0.1711 
                 0.0085 
               
               
                 82 
                 Hs.533185 
                 NM_002358 
                 MAD2L1 
                 0.0608 
                 0.0898 
                 0.6773 
               
               
                 83 
                 Hs.19400 
                 NM_006341 
                 
                   MAD2L2 
                 
                 
                   0.0154 
                 
                 
                   0.1848 
                 
                 
                   0.0835 
                 
               
               
                 84 
                 Hs.477481 
                 NM_004526 
                 
                   MCM2 
                 
                 
                   0.0791 
                 
                 
                   0.1746 
                 
                 
                   0.4533 
                 
               
               
                 85 
                 Hs.179565 
                 NM_002388 
                 MCM3 
                 0.0018 
                 −0.0213 
                 −0.0825 
               
               
                 86 
                 Hs.460184 
                 NM_005914 
                 MCM4 
                 0.0201 
                 −0.0149 
                 −1.3454 
               
               
                 87 
                 Hs.517582 
                 NM_006739 
                 
                   MCM5 
                 
                 
                   0.1204 
                 
                 
                   0.0107 
                 
                 
                   11.2919 
                 
               
               
                 88 
                 Hs.444118 
                 NM_005915 
                 MCM6 
                 0.0735 
                 0.0216 
                 3.3983 
               
               
                 89 
                 Hs.438720 
                 NM_005916 
                 MCM7 
                 0.0617 
                 0.0989 
                 0.6241 
               
               
                 90 
                 Hs.80976 
                 NM_002417 
                 
                   MKI67 
                 
                 
                   0.1220 
                 
                 
                   0.0134 
                 
                 
                   9.0981 
                 
               
               
                 91 
                 Hs.509523 
                 NM_002431 
                 MNAT1 
                 0.0192 
                 0.0200 
                 0.9562 
               
               
                 92 
                 Hs.20555 
                 NM_005590 
                 MRE11A 
                 0.0372 
                 −0.0350 
                 −1.0608 
               
               
                 93 
                 Hs.492208 
                 NM_002485 
                 NBS1 
                 0.0732 
                 −0.0394 
                 −1.8589 
               
               
                 94 
                 Hs.147433 
                 NM_182649 
                 
                   PCNA 
                 
                 
                   0.1419 
                 
                 
                   0.0591 
                 
                 
                   2.3998 
                 
               
               
                 95 
                 Hs.77783 
                 NM_182687 
                 
                   PKMYT1 
                 
                 
                   0.1034 
                 
                 
                   0.0472 
                 
                 
                   2.1880 
                 
               
               
                 96 
                 Hs.531879 
                 NM_002853 
                 
                   RAD1 
                 
                 
                   0.1034 
                 
                 
                   0.0308 
                 
                 
                   3.3579 
                 
               
               
                 97 
                 Hs.16184 
                 NM_002873 
                 RAD17 
                 0.0331 
                 0.0141 
                 2.3512 
               
               
                 98 
                 Hs.242635 
                 NM_005732 
                 RAD50 
                 0.0540 
                 −0.0636 
                 −0.8481 
               
               
                 99 
                 Hs.446554 
                 NM_002875 
                 RAD51 
                 0.0058 
                 −0.0325 
                 −0.1783 
               
               
                 100 
                 Hs.240457 
                 NM_004584 
                 RAD9A 
                 −0.0085 
                 −0.0188 
                 0.4513 
               
               
                 101 
                 Hs.408528 
                 NM_000321 
                 RB1 
                 0.3510 
                 0.2383 
                 1.4727 
               
               
                 102 
                 Hs.546282 
                 NM_002894 
                 
                   RBBP8 
                 
                 
                   0.1354 
                 
                 
                   0.0173 
                 
                 
                   7.8265 
                 
               
               
                 103 
                 Hs.207745 
                 NM_002895 
                 RBL1 
                 0.0639 
                 0.0749 
                 0.8532 
               
               
                 104 
                 Hs.513609 
                 NM_005611 
                 
                   RBL2 
                 
                 
                   0.1556 
                 
                 
                   0.0706 
                 
                 
                   2.2048 
                 
               
               
                 105 
                 Hs.507866 
                 NM_014059 
                 RGC32 
                 −0.0243 
                 0.0751 
                 −0.3240 
               
               
                 106 
                 Hs.487540 
                 NM_002947 
                 RPA3 
                 0.0275 
                 −0.0382 
                 −0.7203 
               
               
                 107 
                 Hs.269898 
                 NM_013376 
                 SERTAD1 
                 0.0272 
                 −0.0819 
                 −0.3326 
               
               
                 108 
                 Hs.23348 
                 NM_005983 
                 SKP2 
                 0.0533 
                 −0.1075 
                 −0.4961 
               
               
                 109 
                 Hs.81424 
                 NM_003352 
                 
                   SUMO1 
                 
                 
                   0.1121 
                 
                 
                   0.0468 
                 
                 
                   2.3954 
                 
               
               
                 110 
                 Hs.79353 
                 NM_007111 
                 TFDP1 
                 0.0645 
                 −0.0069 
                 −9.3060 
               
               
                 111 
                 Hs.379018 
                 NM_006286 
                 
                   TFDP2 
                 
                 
                   0.1382 
                 
                 
                   0.0504 
                 
                 
                   2.7391 
                 
               
               
                 112 
                 Hs.408312 
                 NM_000546 
                 TP53 
                 0.3084 
                 0.1853 
                 1.6645 
               
               
                 113 
                 Hs.406693 
                 NM_003334 
                 
                   UBE1 
                 
                 
                   0.0257 
                 
                 
                   0.1261 
                 
                 
                   0.2037 
                 
               
               
                 114 
                   
                   
                 Blank 
                 0.0428 
                 0.0150 
                 2.8497 
               
               
                 115 
                 N/A 
                 L08752 
                 PUC18 
                 0.1037 
                 0.0852 
                 1.2171 
               
               
                 116 
                   
                   
                 Blank 
                 −0.0228 
                 −0.0449 
                 0.5077 
               
               
                 117 
                   
                   
                 Blank 
                 −0.0200 
                 0.0299 
                 −0.6692 
               
               
                 118 
                 N/A 
                 N/A 
                 AS1R2 
                 0.0319 
                 0.0395 
                 0.8082 
               
               
                 119 
                 N/A 
                 N/A 
                 AS1R1 
                 0.3081 
                 0.1775 
                 1.7356 
               
               
                 120 
                 N/A 
                 N/A 
                 AS1 
                 0.8820 
                 0.7096 
                 1.2428 
               
               
                 121 
                 Hs.544577 
                 NM_002046 
                 GAPD 
                 0.8549 
                 1.3506 
                 0.6330 
               
               
                 122 
                 Hs.534255 
                 NM_004048 
                 B2M 
                 0.2289 
                 0.1485 
                 1.5413 
               
               
                 123 
                 Hs.509736 
                 NM_007355 
                 HSPCB 
                 0.7179 
                 1.0587 
                 0.6781 
               
               
                 124 
                 Hs.509736 
                 NM_007355 
                 HSPCB 
                 0.7975 
                 0.9087 
                 0.8776 
               
               
                 125 
                 Hs.520640 
                 NM_001101 
                 ACTB 
                 1.6684 
                 1.4658 
                 1.1382 
               
               
                 126 
                 Hs.520640 
                 NM_001101 
                 ACTB 
                 1.7616 
                 1.3268 
                 1.3277 
               
               
                 127 
                 N/A 
                 N/A 
                 BAS2C 
                 2.1083 
                 1.4589 
                 1.4451 
               
               
                 128 
                 N/A 
                 N/A 
                 BAS2C 
                 2.0903 
                 1.4185 
                 1.4736 
               
               
                   
               
               
                 * As default, Group 1 is used as control group. 
               
             
          
         
       
     
         [0000]    
       
         
               
               
               
             
               
               
               
               
             
               
               
               
               
               
               
               
             
           
               
                   
                   
               
             
             
               
                   
                 Project 
                 Pao Pereira DNA damage 100 ug/ml Pao, 24 hr, LNCaP 
               
               
                   
                 Catalog # 
                 Oligo GEArray ® Human DNA Damage Signaling Pathway 
               
               
                   
                   
                 Microarray 
               
               
                   
                 Density 
                 Average 
               
               
                   
                 Clover 
                 Clover Off 
               
               
                   
                 Total Arrays 
                 2 
               
               
                   
                 Total Groups 
                 2 
               
               
                   
                 Background 
                 Empty spots: positions 117, 116 
               
               
                   
                 Normalization 
                 Selected genes: positions 1, 121, 123, 124, 125, 126 
               
               
                   
                 Array Name 
                 Pao Rx DNA damage scan dark.tif 
               
             
          
           
               
                   
                 Assigned Group 
                 Group 1 
                 Group 2 
               
               
                   
                   
               
             
          
           
               
                 Position 
                 UniGene 
                 RefSeq # 
                 Symbol 
                 Group 2 
                 Group 1 
                 Group 2/Group 1 
               
               
                   
               
               
                 1 
                 Hs.546292 
                 NM_002954 
                 RPS27A 
                 0.776 
                 0.898 
                 0.864 
               
               
                 2 
                 Hs.431048 
                 NM_005157 
                 ABL1 
                 −0.000 
                 0.057 
                 −0.006 
               
               
                 3 
                 Hs.518804 
                 NM_198889 
                 ANKRD17 
                 0.004 
                 0.010 
                 0.467 
               
               
                 4 
                 Hs.73722 
                 NM_080649 
                 
                   APEX1 
                 
                 
                   0.009 
                 
                 
                   0.113 
                 
                 
                   0.080 
                 
               
               
                 5 
                 Hs.154149 
                 NM_014481 
                 APEX2 
                 −0.044 
                 −0.017 
                 2.615 
               
               
                 6 
                 Hs.435561 
                 NM_000051 
                 
                   ATM 
                 
                 
                   0.335 
                 
                 
                   0.181 
                 
                 
                   1.857 
                 
               
               
                 7 
                 Hs.271791 
                 NM_001184 
                 ATR 
                 −0.015 
                 −0.031 
                 0.501 
               
               
                 8 
                 Hs.533526 
                 NM_000489 
                 ATRX 
                 0.024 
                 0.009 
                 2.752 
               
               
                 9 
                 Hs.194143 
                 NM_007294 
                 
                   BRCA1 
                 
                 
                   1.480 
                 
                 
                   0.030 
                 
                 
                   49.394 
                 
               
               
                 10 
                 Hs.34012 
                 NM_000059 
                 BRCA2 
                 0.020 
                 0.034 
                 0.600 
               
               
                 11 
                 Hs.519162 
                 NM_006763 
                 BTG2 
                 0.088 
                 −0.029 
                 −3.060 
               
               
                 12 
                 Hs.146607 
                 NM_001239 
                 CCNH 
                 0.065 
                 0.093 
                 0.695 
               
               
                 13 
                 Hs.184298 
                 NM_001799 
                 CDK7 
                 0.019 
                 −0.025 
                 −0.775 
               
               
                 14 
                 Hs.24529 
                 NM_001274 
                 CHEK1 
                 −0.002 
                 −0.034 
                 0.047 
               
               
                 15 
                 Hs.291363 
                 NM_007194 
                 CHEK2 
                 −0.005 
                 −0.054 
                 0.100 
               
               
                 16 
                 Hs.135471 
                 NM_006384 
                 
                   CIB1 
                 
                 
                   0.317 
                 
                 
                   0.826 
                 
                 
                   0.384 
                 
               
               
                 17 
                 Hs.249129 
                 NM_001279 
                 CIDEA 
                 −0.014 
                 0.033 
                 −0.426 
               
               
                 18 
                 Hs.448590 
                 NM_014430 
                 CIDEB 
                 −0.006 
                 0.054 
                 −0.112 
               
               
                 19 
                 Hs.435237 
                 NM_000082 
                 ERCC8 
                 0.065 
                 −0.010 
                 −6.217 
               
               
                 20 
                 Hs.151573 
                 NM_004075 
                 CRY1 
                 0.061 
                 −0.025 
                 −2.399 
               
               
                 21 
                 Hs.532491 
                 NM_021117 
                 CRY2 
                 0.030 
                 −0.084 
                 −0.357 
               
               
                 22 
                 Hs.290758 
                 NM_001923 
                 DDB1 
                 −0.005 
                 −0.063 
                 0.076 
               
               
                 23 
                 Hs.446564 
                 NM_000107 
                 DDB2 
                 −0.022 
                 −0.072 
                 0.309 
               
               
                 24 
                 Hs.505777 
                 NM_004083 
                 
                   DDIT3 
                 
                 
                   0.876 
                 
                 
                   0.035 
                 
                 
                   24.790 
                 
               
               
                 25 
                 Hs.339396 
                 NM_007068 
                 DMC1 
                 −0.047 
                 0.041 
                 −1.139 
               
               
                 26 
                 Hs.435981 
                 NM_001983 
                 
                   ERCCI 
                 
                 
                   0.044 
                 
                 
                   0.132 
                 
                 
                   0.333 
                 
               
               
                 27 
                 Hs.487294 
                 NM_000400 
                 ERCC2 
                 0.055 
                 −0.036 
                 −1.514 
               
               
                 28 
                 Hs.469872 
                 NM_000122 
                 ERCC3 
                 0.161 
                 −0.024 
                 −6.716 
               
               
                 29 
                 Hs.460019 
                 NM_005236 
                 ERCC4 
                 0.028 
                 −0.075 
                 −0.368 
               
               
                 30 
                 Hs.258429 
                 NM_000123 
                 ERCC5 
                 −0.069 
                 −0.050 
                 1.378 
               
               
                 31 
                 Hs.498248 
                 NM_130398 
                 EXO1 
                 −0.066 
                 −0.098 
                 0.675 
               
               
                 32 
                 Hs.434873 
                 NM_004629 
                 FANCG 
                 −0.044 
                 −0.082 
                 0.528 
               
               
                 33 
                 Hs.409065 
                 NM_004111 
                 FEN1 
                 0.083 
                 0.074 
                 1.113 
               
               
                 34 
                 Hs.292493 
                 NM_001469 
                 G22P1 
                 1.067 
                 0.974 
                 1.095 
               
               
                 35 
                 Hs.80409 
                 NM_001924 
                 
                   GADD45A 
                 
                 
                   0.379 
                 
                 
                   0.047 
                 
                 
                   8.015 
                 
               
               
                 36 
                 Hs.9701 
                 NM_006705 
                 GADD45G 
                 0.193 
                 −0.030 
                 −6.488 
               
               
                 37 
                 Hs.86161 
                 NM_002066 
                 GML 
                 0.068 
                 −0.059 
                 −1.138 
               
               
                 38 
                 Hs.523510 
                 NM_005316 
                 GTF2H1 
                 0.024 
                 −0.016 
                 −1.519 
               
               
                 39 
                 Hs.191356 
                 NM_001515 
                 GTF2H2 
                 0.107 
                 0.161 
                 0.661 
               
               
                 40 
                 Hs.355348 
                 NM_001516 
                 GTF2H3 
                 −0.108 
                 −0.011 
                 9.991 
               
               
                 41 
                 Hs.386189 
                 NM_016426 
                 GTSE1 
                 0.044 
                 −0.004 
                 −10.361 
               
               
                 42 
                 Hs.152983 
                 NM_004507 
                 HUS1 
                 0.099 
                 −0.003 
                 −36.939 
               
               
                 43 
                 Hs.503048 
                 NM_002180 
                 IGHMBP2 
                 0.105 
                 −0.024 
                 −4.407 
               
               
                 44 
                 Hs.17253 
                 NM_054111 
                 IHPK3 
                 0.019 
                 −0.065 
                 −0.295 
               
               
                 45 
                 Hs.523875 
                 NM_001567 
                 INPPL1 
                 0.007 
                 −0.067 
                 −0.111 
               
               
                 46 
                 Hs.61188 
                 NM_033276 
                 KUB3 
                 −0.033 
                 −0.030 
                 1.104 
               
               
                 47 
                 Hs.1770 
                 NM_000234 
                 LIG1 
                 0.028 
                 −0.033 
                 −0.851 
               
               
                 48 
                 Hs.100299 
                 NM_002311 
                 LIG3 
                 −0.039 
                 −0.031 
                 1.263 
               
               
                 49 
                 Hs.166091 
                 NM_002312 
                 LIG4 
                 0.052 
                 0.006 
                 8.808 
               
               
                 50 
                 Hs.463978 
                 NM_002758 
                 
                   MAP2K6 
                 
                 
                   0.046 
                 
                 
                   0.111 
                 
                 
                   0.413 
                 
               
               
                 51 
                 Hs.432642 
                 NM_002969 
                 MAPK12 
                 0.126 
                 −0.026 
                 −4.904 
               
               
                 52 
                 Hs.35947 
                 NM_003925 
                 MBD4 
                 0.036 
                 −0.053 
                 −0.694 
               
               
                 53 
                 Hs.195364 
                 NM_000249 
                 MLH1 
                 0.090 
                 0.030 
                 2.994 
               
               
                 54 
                 Hs.279843 
                 NM_014381 
                 MLH3 
                 −0.175 
                 −0.034 
                 5.158 
               
               
                 55 
                 Hs.509523 
                 NM_002431 
                 MNAT1 
                 −0.044 
                 0.008 
                 −5.234 
               
               
                 56 
                 Hs.459596 
                 NM_002434 
                 MPG 
                 −0.197 
                 0.154 
                 −1.278 
               
               
                 57 
                 Hs.20555 
                 NM_005590 
                 
                   MRE11A 
                 
                 
                   0.125 
                 
                 
                   0.017 
                 
                 
                   7.281 
                 
               
               
                 58 
                 Hs.156519 
                 NM_000251 
                 MSH2 
                 0.126 
                 0.072 
                 1.757 
               
               
                 59 
                 Hs.280987 
                 NM_002439 
                 
                   MSH3 
                 
                 
                   0.124 
                 
                 
                   0.011 
                 
                 
                   10.811 
                 
               
               
                 60 
                 Hs.216639 
                 NM_002440 
                 MSH4 
                 0.114 
                 −0.050 
                 −2.272 
               
               
                 61 
                 Hs.371225 
                 NM_002441 
                 MSH5 
                 0.315 
                 0.167 
                 1.888 
               
               
                 62 
                 Hs.445052 
                 NM_000179 
                 MSH6 
                 0.082 
                 0.041 
                 1.997 
               
               
                 63 
                 Hs.271353 
                 NM_012222 
                 MUTYH 
                 0.042 
                 0.035 
                 1.181 
               
               
                 64 
                 Hs.396494 
                 NM_018177 
                 N4BP2 
                 −0.032 
                 0.047 
                 −0.675 
               
               
                 65 
                 Hs.492208 
                 NM_002485 
                 NBS1 
                 0.070 
                 0.008 
                 8.919 
               
               
                 66 
                 Hs.66196 
                 NM_002528 
                 NTHL1 
                 0.098 
                 0.083 
                 1.181 
               
               
                 67 
                 Hs.534331 
                 NM_002452 
                 
                   NUDT1 
                 
                 
                   0.173 
                 
                 
                   0.039 
                 
                 
                   4.398 
                 
               
               
                 68 
                 Hs.380271 
                 NM_002542 
                 OGG1 
                 0.112 
                 −0.003 
                 −41.960 
               
               
                 69 
                 Hs.20930 
                 NM_020418 
                 PCBP4 
                 0.124 
                 −0.054 
                 −2.315 
               
               
                 70 
                 Hs.147433 
                 NM_182649 
                 PCNA 
                 0.253 
                 0.154 
                 1.646 
               
               
                 71 
                 Hs.424932 
                 NM_004208 
                 
                   PDCD8 
                 
                 
                   0.125 
                 
                 
                   0.020 
                 
                 
                   6.383 
                 
               
               
                 72 
                 Hs.111749 
                 NM_000534 
                 PMS1 
                 0.099 
                 0.057 
                 1.733 
               
               
                 73 
                 Hs.487470 
                 NM_000535 
                 PMS2 
                 0.084 
                 −0.006 
                 −13.301 
               
               
                 74 
                 Hs.278468 
                 D38500 
                 PMS2L4 
                 0.110 
                 0.033 
                 3.311 
               
               
                 75 
                 Hs.278467 
                 NM_005395 
                 PMS2L9 
                 0.089 
                 −0.001 
                 −147.706 
               
               
                 76 
                 Hs.78016 
                 NM_007254 
                 PNKP 
                 0.056 
                 −0.035 
                 −1.608 
               
               
                 77 
                 Hs.76556 
                 NM_014330 
                 PPP1R15A 
                 0.025 
                 −0.051 
                 −0.486 
               
               
                 78 
                 Hs.491682 
                 NM_006904 
                 PRKDC 
                 0.034 
                 0.001 
                 44.059 
               
               
                 79 
                 Hs.531879 
                 NM_002853 
                 RAD1 
                 0.057 
                 −0.026 
                 −2.234 
               
               
                 80 
                 Hs.16184 
                 NM_002873 
                 RAD17 
                 −0.038 
                 0.027 
                 −1.397 
               
               
                 81 
                 Hs.375684 
                 NM_020165 
                 RAD18 
                 0.155 
                 −0.045 
                 −3.469 
               
               
                 82 
                 Hs.81848 
                 NM_006265 
                 RAD21 
                 0.094 
                 0.023 
                 4.143 
               
               
                 83 
                 Hs.440960 
                 NM_005053 
                 RAD23A 
                 0.095 
                 0.001 
                 157.765 
               
               
                 84 
                 Hs.521640 
                 NM_002874 
                 
                   RAD23B 
                 
                 
                   0.206 
                 
                 
                   0.048 
                 
                 
                   4.307 
                 
               
               
                 85 
                 Hs.242635 
                 NM_005732 
                 RAD50 
                 −0.024 
                 −0.110 
                 0.219 
               
               
                 86 
                 Hs.446554 
                 NM_002875 
                 RAD51 
                 0.028 
                 −0.037 
                 −0.776 
               
               
                 87 
                 Hs.412587 
                 NM_058216 
                 RAD51C 
                 0.063 
                 −0.004 
                 −14.315 
               
               
                 88 
                 Hs.172587 
                 NM_133509 
                 RAD51L1 
                 −0.146 
                 0.018 
                 −8.225 
               
               
                 89 
                 Hs.125244 
                 NM_002878 
                 RAD51L3 
                 0.069 
                 −0.039 
                 −1.759 
               
               
                 90 
                 Hs.552577 
                 NM_002879 
                 RAD52 
                 0.064 
                 −0.025 
                 −2.530 
               
               
                 91 
                 Hs.523220 
                 NM_003579 
                 RAD54L 
                 0.004 
                 −0.001 
                 −5.353 
               
               
                 92 
                 Hs.240457 
                 NM_004584 
                 RAD9A 
                 0.088 
                 −0.050 
                 −1.754 
               
               
                 93 
                 Hs.546282 
                 NM_002894 
                 RBBP8 
                 −0.031 
                 −0.061 
                 0.511 
               
               
                 94 
                 Hs.443077 
                 NM_016316 
                 REV1L 
                 0.085 
                 −0.054 
                 −1.572 
               
               
                 95 
                 Hs.461925 
                 NM_002945 
                 RPA1 
                 0.044 
                 0.041 
                 1.081 
               
               
                 96 
                 Hs.487540 
                 NM_002947 
                 RPA3 
                 −0.120 
                 −0.012 
                 10.294 
               
               
                 97 
                 Hs.408846 
                 NM_022367 
                 SEMA4A 
                 0.030 
                 −0.019 
                 −1.603 
               
               
                 98 
                 Hs.59554 
                 NM_014454 
                 SESN1 
                 −0.002 
                 −0.028 
                 0.056 
               
               
                 99 
                 Hs.211602 
                 NM_006306 
                 SMC1L1 
                 −0.047 
                 −0.025 
                 1.911 
               
               
                 100 
                 Hs.81424 
                 NM_003352 
                 
                   SUMO1 
                 
                 
                   0.138 
                 
                 
                   0.015 
                 
                 
                   8.923 
                 
               
               
                 101 
                 Hs.408312 
                 NM_000546 
                 TP53 
                 0.182 
                 0.288 
                 0.631 
               
               
                 102 
                 Hs.192132 
                 NM_005427 
                 TP73 
                 −0.025 
                 −0.100 
                 0.246 
               
               
                 103 
                 Hs.344812 
                 NM_016381 
                 TREX1 
                 −0.012 
                 −0.004 
                 3.189 
               
               
                 104 
                 Hs.170835 
                 NM_007205 
                 TREX2 
                 −0.089 
                 −0.026 
                 3.446 
               
               
                 105 
                 Hs.191334 
                 NM_003362 
                 UNG 
                 −0.049 
                 −0.057 
                 0.866 
               
               
                 106 
                 Hs.3041 
                 NM_021147 
                 UNG2 
                 −0.027 
                 −0.031 
                 0.866 
               
               
                 107 
                 Hs.288867 
                 NM_000380 
                 XPA 
                 −0.003 
                 −0.043 
                 0.059 
               
               
                 108 
                 Hs.475538 
                 NM_004628 
                 XPC 
                 0.069 
                 −0.037 
                 −1.883 
               
               
                 109 
                 Hs.98493 
                 NM_006297 
                 XRCC1 
                 0.063 
                 −0.034 
                 −1.839 
               
               
                 110 
                 Hs.129727 
                 NM_005431 
                 XRCC2 
                 0.020 
                 −0.051 
                 −0.399 
               
               
                 111 
                 Hs.549075 
                 NM_005432 
                 XRCC3 
                 −0.059 
                 −0.060 
                 0.979 
               
               
                 112 
                 Hs.171190 
                 NM_003401 
                 XRCC4 
                 0.008 
                 0.082 
                 0.098 
               
               
                 113 
                 Hs.388739 
                 NM_021141 
                 XRCC5 
                 0.086 
                 0.131 
                 0.659 
               
               
                 114 
                 Hs.444451 
                 NM_016653 
                 ZAK 
                 0.062 
                 −0.056 
                 −1.124 
               
               
                 115 
                 N/A 
                 L08752 
                 PUC18 
                 −0.010 
                 0.002 
                 −6.046 
               
               
                 116 
                   
                   
                 Blank 
                 0.030 
                 −0.019 
                 −1.565 
               
               
                 117 
                   
                   
                 Blank 
                 −0.030 
                 0.019 
                 −1.565 
               
               
                 118 
                 N/A 
                 N/A 
                 AS1R2 
                 0.024 
                 −0.031 
                 −0.772 
               
               
                 119 
                 N/A 
                 N/A 
                 AS1R1 
                 0.034 
                 0.074 
                 0.461 
               
               
                 120 
                 N/A 
                 N/A 
                 AS1 
                 0.372 
                 0.249 
                 1.495 
               
               
                 121 
                 Hs.544577 
                 NM_002046 
                 GAPD 
                 0.830 
                 0.949 
                 0.874 
               
               
                 122 
                 Hs.534255 
                 NM_004048 
                 B2M 
                 0.220 
                 0.034 
                 6.413 
               
               
                 123 
                 Hs.509736 
                 NM_007355 
                 HSPCB 
                 0.873 
                 0.846 
                 1.031 
               
               
                 124 
                 Hs.509736 
                 NM_007355 
                 HSPCB 
                 0.856 
                 1.072 
                 0.799 
               
               
                 125 
                 Hs.520640 
                 NM_001101 
                 ACTB 
                 1.400 
                 1.114 
                 1.257 
               
               
                 126 
                 Hs.520640 
                 NM_001101 
                 ACTB 
                 1.265 
                 1.120 
                 1.129 
               
               
                 127 
                 N/A 
                 N/A 
                 BAS2C 
                 2.082 
                 1.110 
                 1.875 
               
               
                 128 
                 N/A 
                 N/A 
                 BAS2C 
                 2.005 
                 1.061 
                 1.889 
               
               
                   
               
               
                 * As default, Group 1 is used as control group. 
               
             
          
         
       
     
         [0000]    
       
         
               
               
               
             
               
               
               
               
             
               
               
               
               
               
               
               
             
           
               
                   
                   
               
             
             
               
                   
                 Project 
                 Rauwolfia DNA damage 500 ug/ml 24 hr LNCaP 
               
               
                   
                 Catalog # 
                 Oligo GEArray ® Human DNA Damage Signaling Pathway 
               
               
                   
                   
                 Microarray 
               
               
                   
                 Density 
                 Average 
               
               
                   
                 Clover 
                 Clover Off 
               
               
                   
                 Total Arrays 
                 2 
               
               
                   
                 Total Groups 
                 2 
               
               
                   
                 Background 
                 Empty spots: positions 117, 116 
               
               
                   
                 Normalization 
                 Selected genes: positions 1, 121, 123, 124, 125, 126 
               
               
                   
                 Array Name 
                 Rauw Rx DNA damage scans.tif1 
               
             
          
           
               
                   
                 Assigned Group 
                 Group 1 
                 Group 2 
               
               
                   
                   
               
             
          
           
               
                 Position 
                 UniGene 
                 RefSeq # 
                 Symbol 
                 Group 2 
                 Group 1 
                 Group 2/Group 1 
               
               
                   
               
               
                 1 
                 Hs.546292 
                 NM_002954 
                 RPS27A 
                 0.6354 
                 0.9338 
                 0.6805 
               
               
                 2 
                 Hs.431048 
                 NM_005157 
                 ABL1 
                 −0.0417 
                 0.0205 
                 −2.0340 
               
               
                 3 
                 Hs.518804 
                 NM_198889 
                 ANKRD17 
                 −0.0848 
                 −0.0055 
                 15.4607 
               
               
                 4 
                 Hs.73722 
                 NM_080649 
                 APEX1 
                 −0.0515 
                 0.1662 
                 −0.3097 
               
               
                 5 
                 Hs.154149 
                 NM_014481 
                 APEX2 
                 −0.0692 
                 0.0076 
                 −9.1234 
               
               
                 6 
                 Hs.435561 
                 NM_000051 
                 
                   ATM 
                 
                 
                   0.0617 
                 
                 
                   0.1933 
                 
                 
                   0.3191 
                 
               
               
                 7 
                 Hs.271791 
                 NM_001184 
                 ATR 
                 −0.0596 
                 −0.0426 
                 1.3992 
               
               
                 8 
                 Hs.533526 
                 NM_000489 
                 
                   ATRX 
                 
                 
                   0.1284 
                 
                 
                   −0.0299 
                 
                 
                   −4.3005 
                 
               
               
                 9 
                 Hs.194143 
                 NM_007294 
                 BRCA1 
                 0.1155 
                 0.1689 
                 0.6834 
               
               
                 10 
                 Hs.34012 
                 NM_000059 
                 BRCA2 
                 −0.0404 
                 0.0103 
                 −3.9150 
               
               
                 11 
                 Hs.519162 
                 NM_006763 
                 BTG2 
                 −0.0746 
                 −0.0084 
                 8.8919 
               
               
                 12 
                 Hs.146607 
                 NM_001239 
                 
                   CCNH 
                 
                 
                   −0.0194 
                 
                 
                   0.1838 
                 
                 
                   −0.1055 
                 
               
               
                 13 
                 Hs.184298 
                 NM_001799 
                 CDK7 
                 −0.0500 
                 −0.0158 
                 3.1630 
               
               
                 14 
                 Hs.24529 
                 NM_001274 
                 CHEK1 
                 −0.0584 
                 −0.0986 
                 0.5919 
               
               
                 15 
                 Hs.291363 
                 NM_007194 
                 CHEK2 
                 −0.0361 
                 −0.0624 
                 0.5774 
               
               
                 16 
                 Hs.135471 
                 NM_006384 
                 CIB1 
                 0.6782 
                 0.8983 
                 0.7549 
               
               
                 17 
                 Hs.249129 
                 NM_001279 
                 CIDEA 
                 0.0004 
                 0.0297 
                 0.0140 
               
               
                 18 
                 Hs.448590 
                 NM_014430 
                 CIDEB 
                 −0.0294 
                 −0.0242 
                 1.2141 
               
               
                 19 
                 Hs.435237 
                 NM_000082 
                 ERCC8 
                 −0.0554 
                 0.0219 
                 −2.5261 
               
               
                 20 
                 Hs.151573 
                 NM_004075 
                 CRY1 
                 −0.0779 
                 −0.0613 
                 1.2712 
               
               
                 21 
                 Hs.532491 
                 NM_021117 
                 CRY2 
                 −0.0269 
                 −0.0423 
                 0.6359 
               
               
                 22 
                 Hs.290758 
                 NM_001923 
                 DDB1 
                 0.0006 
                 −0.0534 
                 −0.0117 
               
               
                 23 
                 Hs.446564 
                 NM_000107 
                 DDB2 
                 −0.0008 
                 −0.0607 
                 0.0137 
               
               
                 24 
                 Hs.505777 
                 NM_004083 
                 
                   DDIT3 
                 
                 
                   0.9845 
                 
                 
                   0.0360 
                 
                 
                   27.3602 
                 
               
               
                 25 
                 Hs.339396 
                 NM_007068 
                 DMC1 
                 0.0336 
                 −0.0683 
                 −0.4916 
               
               
                 26 
                 Hs.435981 
                 NM_001983 
                 
                   ERCC1 
                 
                 
                   0.0759 
                 
                 
                   0.0155 
                 
                 
                   4.8972 
                 
               
               
                 27 
                 Hs.487294 
                 NM_000400 
                 ERCC2 
                 −0.0131 
                 −0.0434 
                 0.3025 
               
               
                 28 
                 Hs.469872 
                 NM_000122 
                 ERCC3 
                 0.0027 
                 −0.0257 
                 −0.1056 
               
               
                 29 
                 Hs.460019 
                 NM_005236 
                 ERCC4 
                 −0.0313 
                 −0.0660 
                 0.4737 
               
               
                 30 
                 Hs.258429 
                 NM_000123 
                 ERCC5 
                 0.0361 
                 −0.0205 
                 −1.7594 
               
               
                 31 
                 Hs.498248 
                 NM_130398 
                 EXO1 
                 0.0383 
                 −0.0444 
                 −0.8642 
               
               
                 32 
                 Hs.434873 
                 NM_004629 
                 
                   FANCG 
                 
                 
                   0.1994 
                 
                 
                   −0.0245 
                 
                 
                   −8.1317 
                 
               
               
                 33 
                 Hs.409065 
                 NM_004111 
                 FEN1 
                 0.0325 
                 0.1731 
                 0.1878 
               
               
                 34 
                 Hs.292493 
                 NM_001469 
                 G22P1 
                 0.9926 
                 0.9859 
                 1.0068 
               
               
                 35 
                 Hs.80409 
                 NM_001924 
                 GADD45A 
                 0.0417 
                 0.0545 
                 0.7642 
               
               
                 36 
                 Hs.9701 
                 NM_006705 
                 GADD45G 
                 0.0694 
                 0.0820 
                 0.8466 
               
               
                 37 
                 Hs.86161 
                 NM_002066 
                 GML 
                 −0.0117 
                 −0.0211 
                 0.5521 
               
               
                 38 
                 Hs.523510 
                 NM_005316 
                 GTF2H1 
                 0.0296 
                 −0.0150 
                 −1.9721 
               
               
                 39 
                 Hs.191356 
                 NM_001515 
                 GTF2H2 
                 0.0790 
                 0.1478 
                 0.5344 
               
               
                 40 
                 Hs.355348 
                 NM_001516 
                 
                   GTF2H3 
                 
                 
                   0.2003 
                 
                 
                   −0.0452 
                 
                 
                   −4.4328 
                 
               
               
                 41 
                 Hs.386189 
                 NM_016426 
                 
                   GTSE1 
                 
                 
                   0.0411 
                 
                 
                   0.1202 
                 
                 
                   0.3415 
                 
               
               
                 42 
                 Hs.152983 
                 NM_004507 
                 HUS1 
                 0.0671 
                 −0.0718 
                 −0.9346 
               
               
                 43 
                 Hs.503048 
                 NM_002180 
                 IGHMBP2 
                 0.0085 
                 −0.0340 
                 −0.2510 
               
               
                 44 
                 Hs.17253 
                 NM_054111 
                 IHPK3 
                 0.0227 
                 −0.0366 
                 −0.6202 
               
               
                 45 
                 Hs.523875 
                 NM_001567 
                 INPPL1 
                 0.0406 
                 −0.0286 
                 −1.4229 
               
               
                 46 
                 Hs.61188 
                 NM_033276 
                 KUB3 
                 0.0679 
                 −0.0760 
                 −0.8939 
               
               
                 47 
                 Hs.1770 
                 NM_000234 
                 LIG1 
                 0.0361 
                 −0.0508 
                 −0.7093 
               
               
                 48 
                 Hs.100299 
                 NM_002311 
                 
                   LIG3 
                 
                 
                   0.2926 
                 
                 
                   −0.0583 
                 
                 
                   −5.0231 
                 
               
               
                 49 
                 Hs.166091 
                 NM_002312 
                 
                   LIG4 
                 
                 
                   0.0996 
                 
                 
                   −0.0568 
                 
                 
                   −1.7539 
                 
               
               
                 50 
                 Hs.463978 
                 NM_002758 
                 MAP2K6 
                 0.0142 
                 −0.0573 
                 −0.2474 
               
               
                 51 
                 Hs.432642 
                 NM_002969 
                 MAPK12 
                 −0.0177 
                 −0.0729 
                 0.2429 
               
               
                 52 
                 Hs.35947 
                 NM_003925 
                 MBD4 
                 0.0240 
                 −0.0365 
                 −0.6572 
               
               
                 53 
                 Hs.195364 
                 NM_000249 
                 MLH1 
                 0.0377 
                 0.0497 
                 0.7590 
               
               
                 54 
                 Hs.279843 
                 NM_014381 
                 MLH3 
                 0.0338 
                 −0.0473 
                 −0.7141 
               
               
                 55 
                 Hs.509523 
                 NM_002431 
                 MNAT1 
                 0.0754 
                 −0.0192 
                 −3.9289 
               
               
                 56 
                 Hs.459596 
                 NM_002434 
                 
                   MPG 
                 
                 
                   0.2536 
                 
                 
                   0.0287 
                 
                 
                   8.8305 
                 
               
               
                 57 
                 Hs.20555 
                 NM_005590 
                 MRE11A 
                 0.1284 
                 −0.0755 
                 −1.7000 
               
               
                 58 
                 Hs.156519 
                 NM_000251 
                 MSH2 
                 0.0652 
                 −0.0415 
                 −1.5730 
               
               
                 59 
                 Hs.280987 
                 NM_002439 
                 MSH3 
                 0.0213 
                 −0.1175 
                 −0.1810 
               
               
                 60 
                 Hs.216639 
                 NM_002440 
                 MSH4 
                 0.0092 
                 −0.0710 
                 −0.1292 
               
               
                 61 
                 Hs.371225 
                 NM_002441 
                 MSH5 
                 0.1496 
                 0.1609 
                 0.9301 
               
               
                 62 
                 Hs.445052 
                 NM_000179 
                 MSH6 
                 0.1536 
                 0.0513 
                 2.9933 
               
               
                 63 
                 Hs.271353 
                 NM_012222 
                 
                   MUTYH 
                 
                 
                   0.1463 
                 
                 
                   −0.0058 
                 
                 
                   −25.1854 
                 
               
               
                 64 
                 Hs.396494 
                 NM_018177 
                 
                   N4BP2 
                 
                 
                   0.3633 
                 
                 
                   −0.0276 
                 
                 
                   −13.1648 
                 
               
               
                 65 
                 Hs.492208 
                 NM_002485 
                 NBS1 
                 0.0648 
                 −0.0266 
                 −2.4344 
               
               
                 66 
                 Hs.66196 
                 NM_002528 
                 NTHL1 
                 0.0915 
                 −0.0097 
                 −9.4499 
               
               
                 67 
                 Hs.534331 
                 NM_002452 
                 NUDT1 
                 0.0163 
                 −0.1196 
                 −0.1360 
               
               
                 68 
                 Hs.380271 
                 NM_002542 
                 OGG1 
                 0.0125 
                 −0.0127 
                 −0.9809 
               
               
                 69 
                 Hs.20930 
                 NM_020418 
                 PCBP4 
                 0.0681 
                 −0.0424 
                 −1.6059 
               
               
                 70 
                 Hs.147433 
                 NM_182649 
                 PCNA 
                 0.1517 
                 0.1196 
                 1.2689 
               
               
                 71 
                 Hs.424932 
                 NM_004208 
                 
                   PDCD8 
                 
                 
                   0.1836 
                 
                 
                   −0.0066 
                 
                 
                   −27.7528 
                 
               
               
                 72 
                 Hs.111749 
                 NM_000534 
                 
                   PMS1 
                 
                 
                   0.3674 
                 
                 
                   −0.0366 
                 
                 
                   −10.0309 
                 
               
               
                 73 
                 Hs.487470 
                 NM_000535 
                 
                   PMS2 
                 
                 
                   0.1184 
                 
                 
                   −0.0239 
                 
                 
                   −4.9568 
                 
               
               
                 74 
                 Hs.278468 
                 D38500 
                 PMS2L4 
                 0.0536 
                 −0.0026 
                 −20.7457 
               
               
                 75 
                 Hs.278467 
                 NM_005395 
                 PMS2L9 
                 0.0577 
                 −0.0126 
                 −4.5867 
               
               
                 76 
                 Hs.78016 
                 NM_007254 
                 PNKP 
                 0.0571 
                 −0.0010 
                 −58.9812 
               
               
                 77 
                 Hs.76556 
                 NM_014330 
                 PPP1R15A 
                 0.0688 
                 0.0079 
                 8.6983 
               
               
                 78 
                 Hs.491682 
                 NM_006904 
                 PRKDC 
                 0.1077 
                 0.0828 
                 1.3016 
               
               
                 79 
                 Hs.531879 
                 NM_002853 
                 
                   RAD1 
                 
                 
                   0.2147 
                 
                 
                   0.0126 
                 
                 
                   17.0552 
                 
               
               
                 80 
                 Hs.16184 
                 NM_002873 
                 
                   RAD17 
                 
                 
                   0.3462 
                 
                 
                   0.0602 
                 
                 
                   5.7514 
                 
               
               
                 81 
                 Hs.375684 
                 NM_020165 
                 
                   RAD18 
                 
                 
                   0.0844 
                 
                 
                   0.0005 
                 
                 
                   174.3605 
                 
               
               
                 82 
                 Hs.81848 
                 NM_006265 
                 RAD21 
                 0.0554 
                 0.0347 
                 1.5979 
               
               
                 83 
                 Hs.440960 
                 NM_005053 
                 
                   RAD23A 
                 
                 
                   0.0796 
                 
                 
                   0.0040 
                 
                 
                   19.7350 
                 
               
               
                 84 
                 Hs.521640 
                 NM_002874 
                 RAD23B 
                 0.1207 
                 0.1649 
                 0.7317 
               
               
                 85 
                 Hs.242635 
                 NM_005732 
                 RAD50 
                 −0.0102 
                 0.0208 
                 −0.4906 
               
               
                 86 
                 Hs.446554 
                 NM_002875 
                 
                   RAD51 
                 
                 
                   0.1002 
                 
                 
                   0.0224 
                 
                 
                   4.4694 
                 
               
               
                 87 
                 Hs.412587 
                 NM_058216 
                 
                   RAD51C 
                 
                 
                   0.3126 
                 
                 
                   0.0616 
                 
                 
                   5.0716 
                 
               
               
                 88 
                 Hs.172587 
                 NM_133509 
                 RAD51L1 
                 0.2734 
                 0.1962 
                 1.3935 
               
               
                 89 
                 Hs.125244 
                 NM_002878 
                 
                   RAD51L3 
                 
                 
                   0.1080 
                 
                 
                   −0.0608 
                 
                 
                   −1.7746 
                 
               
               
                 90 
                 Hs.552577 
                 NM_002879 
                 RAD52 
                 −0.0046 
                 −0.0423 
                 0.1085 
               
               
                 91 
                 Hs.523220 
                 NM_003579 
                 RAD54L 
                 0.0146 
                 −0.0160 
                 −0.9132 
               
               
                 92 
                 Hs.240457 
                 NM_004584 
                 RAD9A 
                 0.0523 
                 0.0403 
                 1.2967 
               
               
                 93 
                 Hs.546282 
                 NM_002894 
                 RBBP8 
                 −0.0088 
                 0.0158 
                 −0.5535 
               
               
                 94 
                 Hs.443077 
                 NM_016316 
                 REV1L 
                 0.0598 
                 0.0813 
                 0.7355 
               
               
                 95 
                 Hs.461925 
                 NM_002945 
                 
                   RPA1 
                 
                 
                   0.3061 
                 
                 
                   0.1349 
                 
                 
                   2.2695 
                 
               
               
                 96 
                 Hs.487540 
                 NM_002947 
                 
                   RPA3 
                 
                 
                   0.2409 
                 
                 
                   0.0896 
                 
                 
                   2.6902 
                 
               
               
                 97 
                 Hs.408846 
                 NM_022367 
                 SEMA4A 
                 0.0596 
                 −0.0650 
                 −0.9166 
               
               
                 98 
                 Hs.59554 
                 NM_014454 
                 SESN1 
                 0.0046 
                 −0.0878 
                 −0.0522 
               
               
                 99 
                 Hs.211602 
                 NM_006306 
                 SMC1L1 
                 −0.0294 
                 −0.0452 
                 0.6504 
               
               
                 100 
                 Hs.81424 
                 NM_003352 
                 SUMO1 
                 0.0892 
                 0.0858 
                 1.0391 
               
               
                 101 
                 Hs.408312 
                 NM_000546 
                 
                   TP53 
                 
                 
                   0.1676 
                 
                 
                   0.3542 
                 
                 
                   0.4731 
                 
               
               
                 102 
                 Hs.192132 
                 NM_005427 
                 
                   TP73 
                 
                 
                   0.0490 
                 
                 
                   0.0056 
                 
                 
                   8.6719 
                 
               
               
                 103 
                 Hs.344812 
                 NM_016381 
                 TREX1 
                 0.1369 
                 0.1105 
                 1.2388 
               
               
                 104 
                 Hs.170835 
                 NM_007205 
                 
                   TREX2 
                 
                 
                   0.2392 
                 
                 
                   0.0889 
                 
                 
                   2.6909 
                 
               
               
                 105 
                 Hs.191334 
                 NM_003362 
                 UNG 
                 0.0117 
                 −0.0269 
                 −0.4331 
               
               
                 106 
                 Hs.3041 
                 NM_021147 
                 UNG2 
                 −0.0131 
                 −0.0850 
                 0.1544 
               
               
                 107 
                 Hs.288867 
                 NM_000380 
                 XPA 
                 −0.0244 
                 0.0261 
                 −0.9328 
               
               
                 108 
                 Hs.475538 
                 NM_004628 
                 XPC 
                 0.0031 
                 −0.0494 
                 −0.0633 
               
               
                 109 
                 Hs.98493 
                 NM_006297 
                 XRCC1 
                 −0.0210 
                 −0.0313 
                 0.6724 
               
               
                 110 
                 Hs.129727 
                 NM_005431 
                 XRCC2 
                 0.0306 
                 0.0127 
                 2.4033 
               
               
                 111 
                 Hs.549075 
                 NM_005432 
                 
                   XRCC3 
                 
                 
                   0.2405 
                 
                 
                   0.0118 
                 
                 
                   20.4173 
                 
               
               
                 112 
                 Hs.171190 
                 NM_003401 
                 
                   XRCC4 
                 
                 
                   0.2501 
                 
                 
                   0.0694 
                 
                 
                   3.6044 
                 
               
               
                 113 
                 Hs.388739 
                 NM_021141 
                 XRCC5 
                 0.0681 
                 0.0699 
                 0.9754 
               
               
                 114 
                 Hs.444451 
                 NM_016653 
                 ZAK 
                 0.0117 
                 −0.0035 
                 −3.2876 
               
               
                 115 
                 N/A 
                 L08752 
                 PUC18 
                 −0.0390 
                 0.0203 
                 −1.9168 
               
               
                 116 
                   
                   
                 Blank 
                 0.0044 
                 0.0082 
                 0.5318 
               
               
                 117 
                   
                   
                 Blank 
                 −0.0044 
                 −0.0082 
                 0.5318 
               
               
                 118 
                 N/A 
                 N/A 
                 AS1R2 
                 0.0990 
                 0.0157 
                 6.3246 
               
               
                 119 
                 N/A 
                 N/A 
                 AS1R1 
                 0.2345 
                 0.0536 
                 4.3765 
               
               
                 120 
                 N/A 
                 N/A 
                 AS1 
                 0.3899 
                 0.1944 
                 2.0054 
               
               
                 121 
                 Hs.544577 
                 NM_002046 
                 GAPD 
                 0.3374 
                 0.9567 
                 0.3527 
               
               
                 122 
                 Hs.534255 
                 NM_004048 
                 B2M 
                 0.0977 
                 0.0687 
                 1.4219 
               
               
                 123 
                 Hs.509736 
                 NM_007355 
                 HSPCB 
                 0.9589 
                 1.0138 
                 0.9458 
               
               
                 124 
                 Hs.509736 
                 NM_007355 
                 HSPCB 
                 0.9947 
                 1.0253 
                 0.9702 
               
               
                 125 
                 Hs.520640 
                 NM_001101 
                 ACTB 
                 1.5266 
                 1.0338 
                 1.4766 
               
               
                 126 
                 Hs.520640 
                 NM_001101 
                 ACTB 
                 1.5470 
                 1.0366 
                 1.4924 
               
               
                 127 
                 N/A 
                 N/A 
                 BAS2C 
                 1.5366 
                 1.0200 
                 1.5065 
               
               
                 128 
                 N/A 
                 N/A 
                 BAS2C 
                 1.5714 
                 0.9833 
                 1.5980 
               
               
                   
               
               
                 * As default, Group 1 is used as control group.