Abstract:
The inventive composition comprises an active ingredient, for example a quaternary ammonium compound, which active ingredient is capable of inhibiting or destroying a unicellular living organism, such as spermatazoa, bacteria or virus. The active ingredient is combined in a synergistic amount with an activating ingredient capable of inhibiting or destroying the enzymes associated with the unicellular organism. The activating ingredient may be capable of releasing fluoride ions, for example, an inorganic fluoride compound. The composition according to the invention is useful, for example, as a spermicide, or as an antibiotic, or as an anti-STD preparation for local or topical application.

Description:
BACKGROUND OF THE INVENTION 
     The invention relates to the inhibition or destruction of unicellular living creatures such as protozoa, microbes, bacteria, gametes, fungi or others, and viruses. Consequently, it aims in particular at the technical fields of local contraception, of antibiotherapy, antisepsis, as part of either pharmacy or cosmetic and of disinfection. 
     Hereafter, the term &#34;inhibition&#34; of a unicellular living creature or of a virus means either hindering its proliferation, or making it incapable of accomplishing some functions that it usually accomplishes. The term &#34;destroying&#34; means killing the unicellular living creatures or viruses. 
     With regard to the invention, the term &#34;substance&#34; hereafter means any chemical compound or association of chemical compounds having at least one given function or one function common to the compounds, and which can be included in the composition of a finished product, generally associated with one or more excipients and possibly with other substances. Similarly, the term &#34;product&#34; means a usable finished product. Thus, a finished product is generally constituted of at least one excipient and of several substances, each substance being constituted of one or several chemical compounds having similar or identical functions. The term &#34;substance&#34; may correspond to an actual fact, but may be purely theoretical and functional in the case of intricate mixtures where the compounds have multiple effects or which effects interfere with each other. The functional classification in compounds, substances, products does not necessarily correspond to the process of manufacture of the product and to the mixture actually obtained in the practice. As usual, the term &#34;composition&#34; is used here and in all the following text to design a pharmaceutical or cosmetic substance. 
     DESCRIPTION OF THE PRIOR ART 
     Substances inhibiting or destroying unicellular living creatures, either in the biological, pharmaceutical, cosmetic fields, or even in broader fields such as disinfection or others, are already known. 
     In particular, spermicidal compositions are known from U.S. Pat. Nos. 4,339,441 and 4,359,475. 
     Numerous chemical compounds usable in local contraception methods for living beings are also known. In particular, it is known that surfactants lowering the interfacial tension (e.g. quaternary ammoniums, nonoxynols, derivatives of amidoethylglycinate on fatty acids, methyltauride sodium oxysalt . . . ) and some other compounds such as phenylmercuric nitrate, para-menthanylphenylpolyoxyethylene ether or trisodium salt of polysaccharide sulphuric ether, have inhibiting or destroying effects on unicellular living creatures, in particular have spermicidal effects. 
     Substances mechanically acting on the spermatozoa, for example by immobilizing them, are also known. U.S. Pat. No. 4,368,186 describes such effects, and more particularly the association of &#34;poloxamer&#34; with spermatozoa inhibiting agents, in view of controlling gelation and solubilization of the products and thus mechanically increasing the efficiency of the inhibiting agents with an additive or a synergistic effect. 
     Locally contraceptive compositions are also known, particularly spermicidal ones, including such chemical compounds and which are locally usable for inhibiting the fertilization ability of gametes, particularly of spermatozoa of human or animal beings. 
     These known compositions include a concentration of inhibiting chemical compound superior to the minimal inhibiting concentration, called MIC, in solution or suspended in a pharmaceutically acceptable excipient which depends on the galenical form used. It is known that in order to have all the spermatozoa which are contained in 0.2 milliliters of sperm killed in less than 5 seconds (conditions of the total spermicidal test according to the standards of IPPF, International Planned Parenthood Federation), the concentration of the spermicidal active ingredient in 1 milliliter of pharmaceutical composition must be superior or equal to the minimal inhibiting concentration, called MIC, of this active ingredient. The MIC of a chemical compound depends on this compound, but also on the conditions in which it is used, i.e. on the galenical form including the composition. 
     More generally, pharmaceutical or cosmetic compositions including at least one basic active ingredient inhibiting or destroying at least one unicellular living creature and drugs or cosmetic products including such compositions are known. 
     In the field of antisepsis and disinfection, numerous active ingredients are known: halogens (chlorinated or iodinated derivatives . . . ), aldehydes, alcohols, phenols, acids, metals (silver, copper, mercury, zinc salts . . . ), amidines, biguanides, diphenylurea, oxydants (hydrogen peroxide, potassium permanganate . . . ), colouring agents, agents lowering the interfacial tension and wetting agents (cationic, anionic, amphoteric or organic). 
     Antiseptic and/or disinfectant and/or antiprotozoal and/or antifungal and/or antibiotic and/or antiviral products including such inhibiting or destroying substances, and manufacturing processes of these substances or compositions, are also known. 
     The known substances or compositions are generally satisfactory but set some problems for their practical use. 
     These problems are essentially the following: 
     First of all, it is generally desirable to obtain a total inhibition or destruction efficiency of the unicellular living creature(s) or of the viruse(s), i.e. an inhibition or destruction rate of 100% in the practical application circumstances. Well, this is not always the case when no strict precautions for use are taken, or when a limitation of the doses of active ingredient are required (for example in the case of the cosmetic). 
     Moreover, it is generally desirable to exhibit an activity against one or more given unicellular living creatures or viruses, e.g. gametes or pathogens, without giving rise to short-term and/or long-term undesirable side effects (activity against other unicellular living creatures, irritations, damages to the environment . . . ). But, in the practice, it has been found that the doses required for solving the first above mentioned problem give rise to long-term side effects (generally linked to regular use), and even to short-term side effects (use by fits and starts). In the medical field, some teratogen and/or cancerigen effects have been noted from certain threshold doses and with certain active ingredients. In the field of disinfection, some undesirable effects may be noted against the materials to be disinfected. 
     OBJECTS OF THE INVENTION 
     An object of the invention is the increasing of the inhibiting or destroying ability of a substance or composition including a given limited concentration of basic inhibiting or destroying active ingredient, without increasing this concentration. Yet another object of the invention is the reduction of the concentrations of the basic active ingredients without decreasing the inhibiting or destroying ability of the substance or composition for all that and in order to avoid the side effects and to better control the selectivity towards the various unicellular living creatures and viruses. 
     In particular, the invention aims at furnishing a new pharmaceutical or cosmetic composition, more particularly locally contraceptive and a drug or product including such a composition, which may be used either by fits and starts, or regularly and continuously at long-term, without risking side effects such as teratogen or cancerigen, and which efficiency is total, i.e. which percentage of failures is statistically null or insignificant, and this within the regular mere circumstances of use (with no particular precautions). 
     Moreover, the invention aims at furnishing a locally contraceptive pharmaceutical product, easy to use and totally efficient, i.e. which gives some results similar to those of the oral contraception without having the drawbacks thereof. 
     The invention also aims at providing a chemical compound usable in a local contraception method. 
     Finally, another objectif of the invention is the reduction of the doses of the active ingredients inhibiting or destroying unicellular living creatures or viruses in the drugs, cosmetic products, or disinfectant products, especially in the antiseptic, antibiotic, bactericidal, antiprotozoal, antifungal, spermicidal, antiviral . . . products. 
     SUMMARY OF THE INVENTION 
     The invention relates to a substance inhibiting or destroying at least one unicellular living creature or virus in particular such as protozoan, microbe, bacterium, gamete, fungus or other, characterized in that it comprises at least one basic active ingredient inhibiting or destroying said unicellular living creature or virus, and at least one ingredient inhibiting or destroying at least one enzyme associated to said living creature or virus, said latter ingredient being an activator --preferably having a synergistic effect-- of said former basic active ingredient. 
     Preferably, the ingredient inhibiting or destroying at least one associated enzyme, called hereafter &#34;activating ingredient&#34; or &#34;activator&#34; is an agent hindering the working of the couple enzyme/substrate. 
     But it has been surprisingly found that such an agent could advantageously be formed with fluoride anion F -  emitted from a fluorinated compound, either spontaneously, or after enzymic action. It has also been found that, in some cases, the basic active ingredient itself is capable of emitting an activating ingredient, and/or that the activating ingredient itself is an active ingredient inhibiting or destroying at least one unicellular living creature or virus. 
     More precisely, it has been noted that such a fluorinated compound has by itself an ability for inhibiting or destroying unicellular living creatures and viruses, and that such a fluorinated compound has a synergistic effect with the associated basic active ingredient(s). 
     A feature of the invention is to provide a local contraceptive composition, more particularly spermicidal composition, characterized in that it comprises at least one chemical compound capable of emitting fluoride anion F - , as an active ingredient inhibiting or destroying gametes, directly or by potentiation, and an excipient. 
     The invention also provides a drug, an antiseptic and/or disinfectant --more particularly locally antiseptic--, and/or antiprotozoal --more particularly locally antiprotozoal--, and/or bactericide --more particularly locally bactericide--, and/or antifungal --more particularly locally antifungal--, and/or antibiotic --more particularly locally antibiotic--, and/or antiviral product, a cosmetic product --more particularly local cosmetic such as a synthetic soap or milk-- and a contraceptive product which is locally applied in contact with gamets --more particularly ovule, cream, gel, solution, foam, tablet, soluble waffle, tampon, vaginal suppository-- characterized in that they comprise a substance or a composition according to the invention. 
     Here and hereafter, the term &#34;local&#34; and its derivatives means that the product is used at a predetermined place to be treated, e.g. the vagina or other anatomic parts, so that the effect is produced in the environment near this place, against unicellular living creatures or viruses that come into contact with the product, and this in opposition to general administrations (e.g. oral administration or parenteral administration). 
     A substance, a composition, a drug, a product, a chemical compound according to the invention are as well directed to local administrations as to general administrations. For example, a spermicide according to the invention may be locally applied. Besides, when wishing to overcome the resistance effects of the pathogenic unicellular living creatures against the regular therapies, one would rather use general administrations of the invention. 
     The invention is also concerned with the use in a cosmetic product of a composition including at least one basic active ingredient and at least one fluorinated chemical compound capable of emitting fluoride anion F -  as active ingredient of said basic active ingredient(s); with the use of a fluorinated chemical compound capable of emitting fluoride anion F -  for manufacturing a substance including at least one basic active ingredient, as activor of said basic active ingredient; with the use of a substance or a composition according to the invention in the manufacture of a drug to be used as antibiotic and/or antiprotozoal and/or bactericide and/or antifungal and/or antiseptic and/or antivival; and with the use of a substance or a composition according to the invention in the manufacture of a local contraceptive for human or animal being, in particular inhibiting or destroying gametes. 
     The invention also provides a manufacturing process of a substance or composition according to the invention inhibiting or destroying at least one unicellular living creature --in particular such as protozoan, microbe, bacteria, gamete, fungus or other, virus--, characterized in that at least one basic active ingredient inhibiting or destroying said unicellular living creature or virus is mixed with at least one ingredient inhibiting or destroying an enzymatic system associated to said living creature or virus, said latter ingredient being an activator of said former basic active ingredient. 
     The invention also provides a chemical compound including ionizable fluorine for use in a local contraception method for living beings, more particularly in a locally contraceptive product for human or animal beings according to the invention or in a substance or a composition according to the invention. 
     According to the invention an activating ingredient inhibiting or destroying at least one enzyme associated to said unicellular living creature or virus induces a remarkable sensibilization of said unicellular living creature or virus to agressions from outside, in particular to the action of said basic active ingredient inhibiting or destroying said unicellular living creature or virus, by &#34;blocking&#34; the couple enzyme/substrate. It is effectively known that each unicellular living creature or virus owns a vital enzymatic system. Besides, unicellular living creatures or viruses may emit, upon some circumstances (e.g. resistance to antibiotic), some particular enzymes providing the destruction of some aggressing agent (e.g. lactamase destroying penicillins), these enzymes being called &#34;protecting enzymes&#34;. The invention lies in inhibiting the function of these enzymes, and thus fragilizing the unicellular living creature by creating the optimal conditions for the efficiency of the active ingredients against it. This brings numerous advantages compared to the prior art, which are one hand to satisfy the aims above mentioned and, on the other hand, to be able to act in a selective manner upon a given unicellular living creature or upon a given family of unicellular living creatures by acting upon a given enzyme or upon a given family of enzymes. In the case of viruses, the invention aims to inhibiting the enzymes that are necessary for their formation and/or their replication. Hereafter &#34;associated enzymes&#34; means vital and/or protecting enzymes of unicellular living creatures and/or viruses. 
     The inventors have determined that fluorine in the ionized state F -  has particularly efficient and benefic effect as activating ingredient against the associated enzymes, and this under trifling regular concentrations. Moreover, ionized fluorine F -   is very common, economical and relatively easy to use. Its manipulations and utilizations are well controlled, as well as the side effects that he may provide according to the doses used. 
     The invention provides a process of inhibition or destruction of at least one unicellular living creature or virus --in particular such as protozoan, microbe, bacterium, gametee, fungus or other--, characterized by the use of at least one basic active ingredient inhibiting or destroying said unicellular living creature and at least one ingredient inhibiting or destroying at least one enzyme associated to said living creature or virus, said latter ingredient being an activator --preferably having a synergistic effect-- of said former active ingredient; and such a process including the use of at least one substance or composition according to the invention. 
     DETAILED DESCRIPTION OF THE INVENTION 
     The invention relates to the inhibition or destruction of unicellular living creatures such as protozoa, microbes, bacteria, gametes, fungi, whether pathogenic or not, and viruses. The invention has mostly two different kinds of uses: either uses in the cosmetic or pharmaceutical field (case of the following examples of spermicides or bactericides acting against pathogenic agents), or uses in more common fields such as agriculture, disinfection (following example) or others. In all the cases, a substance according to the invention is characterized in that it includes at least one basic active ingredient inhibiting or destroying said unicellular living creature or virus, and at least one ingredient inhibiting or destroying at least one enzyme associated to said living creature or virus, said latter ingredient thus being an activator --preferably having a synergetic effect when being itself capable of inhibiting or destroying said living being or virus-- of said former basic active ingredient. 
     The function of the activating ingredient consists in annihilating the working of the enzymatic system associated to said living creature(s) or virus(es). In this way, the inventors have found that such activating ingredient is advantageously an agent hindering the working of the couple enzyme/substrate, preferably anion fluoride F -  emitted from a fluorinated compound, e.g. a metallic derivative of fluorine. Some very good results have been noted with sodium fluoride, calcium fluoride, potassium fluoride, aluminium fluoride, tin fluoride, ammonium fluoride, or sodium monofluorophosphate or other. For example, the following unicellular living creatures or viruses may be concerned with the invention: 
     gametes (spermatozoa, ovula) 
     gram-positive cocci such as staphylococci and streptococci 
     gram-negative cocci such as gonococci 
     gram-positive bacilli 
     gram-negative bacilli such as colibacilli or escherichia coli 
     acid-alcoholo-fast bacilli such as mycobacteria, e.g. mycobacterium smegmatis 
     spiral bacteria such as spirochetes, e.g. treponema 
     miscellaneous bacteria such as chlamydia 
     flagellate protozoa, such as trichomonas 
     miscellaneous yeasts such as candida albicans 
     viruses or retroviruses, such as HIV (Human Immunodeficiency Virus) and Herpes. 
     For example: the following enzymes associated to at least one such unicellular living creature or virus may be concerned with the invention (Table 1): 
     
                       TABLE 1______________________________________ENZYME           SUBSTRATE______________________________________Alkaline Phosphatase             2-naphthyl phosphateEsterase (C4)    2-naphthyl butyrateEsterase Lipase (C8)            2-naphthyl caprylateLipase (C14)     2-naphthyl myristateLeucine arylamidase            L-leucyl-2-naphthylamideValine arylamidase            L-valyl-2-naphthylamideCystine arylamidase            L-cystyl-2-naphthylamideTrypsin          N-benzoyl-DL-arginine-2- - naphthylamideα Chymotrypsine            N-glutaryl-phenylalanine2-naphthylamideAcid phosphatase 2-naphthyl phosphateNaphthol-AS-B1-  Naphthol-AS-B1-phosphatephosphohydrolaseα galactosidase            6-Br-2-naphthyl α D-            galactopyranosideβ galactosidase            2-naphthyl-β D-            galactopyranosideβ glucuronidase            naphthol-AS-B1-βD-glucuronideα glucosidase            2-naphthyl-α D-glucopyrano-            sideβ glucosidase            6-Br-2-naphthyl-β D-            glucopyranosideN-acetyl-β glucosaminidase            1-naphthyl-N-acetyl-βD-            glucosamimnideα mannosidase            6-Br-2-naphthyl-α D-            mannopyranosideα fucosidase            2-naphthyl-αL-fucopyranoside______________________________________ 
    
     A basic active ingredient used in a substance or composition according to the invention is for example a cationic or anionic or amphoteric or non-ionic surfactant lowering the interfacial tension, preferably a quaternary ammonium. Benzalkonium chloride or other alkylbenzalkonium is an example of cationic surfactant. A methyltauride sodium oxysalt is an example of anionic surfactant. A derivative of amidoethylglycinate on fatty acids is an example of amphoteric surfactant. A nonoxynol is an example of non-ionic surfactant. The basic active ingredient may also advantageously be phenylmercuric nitrate or para-menthanylphenylpolyoxyethylene ether or a trisodium salt of polysaccharide sulfuric ether or other (halogen, aldehyde, alcohol, phenol, acid, metal, amidine, biguanide, diphenylurea, oxydant, colouring agent . . . ). 
     In the various tested uses, it has been shown that the alone activating ingredient alone has generally by itself also an activity directly against said unicellular living creature or virus. It has then surprisingly been noted that the association of the activating ingredient with the basic active ingredient has a synergistic effect in so far as the results obtained are not only corresponding to the sum of the results expected from the only presence of the basic active ingredient and of the activating ingredient, but, on the contrary, are superior to this sum. 
     The efficiency of such active ingredients or activators can be measured with the minimal inhibiting concentration called MIC which corresponds to the concentration of the active ingredient inducing the inhibition or the death of the whole unicellular living creatures or viruses in a given time. 
     Advantageously, in a substance according to the invention, the anion fluoride F -  concentration emitted by the activating fluorinated compound is inferior to the minimal inhibiting concentration (MIC) of fluoride anion F -  without basic active ingredient. 
     Similarly, the basic active ingredient concentration in a substance according to the invention can be inferior to the minimal inhibiting concentration (MIC) of this basic active ingredient without activating ingredient. Even so and surprisingly, such a substance, according to the invention, has a total efficiency, i.e. at least equal to the one of substances including either only fluoride anion F -  in a concentration superior or equal to its MIC, or only a basic active ingredient in a concentration superior or equal to its MIC. 
     Besides, the basic active ingredient itself may also be capable of emitting an activating ingredient, such as fluoride anion F - . A substance or a composition according to the invention may include several basic active ingredients which functions may be identical or various and/or several activating ingredients acting on at least one enzyme associated to one or several of the basic active ingredients. 
     A substance or a composition according to the invention can be used in multiple ways for obtaining products, and according to the way of using the products, can be used in local or general administration. 
     Among the particularly advantageous uses of products according to the invention, the various therapeutical possible uses as drug can be mentioned. As a matter of fact, the problem of inhibition or destruction of unicellular living creatures or viruses is encountered in an always increasing number of therapeutical uses. This may be either the fight against some pathogenic agents, and then the products are antibiotic and/or antiprotozoal and/or bactericide and/or antifungal and/or antiseptic and/or antiviral, or even against some non-pathogenic agents such as gametes, e.g. spermatozoa within the framework of contraception, in particular local contraception. 
     A substance or a composition according to the invention can also advantageously be used in a cosmetic product with such doses and concentrations that do not permit its classification as a pharmaceutical product. 
     As a matter of fact, for example, the efficient concentrations of fluorinated chemical compounds and even of basic active ingredients can be lowered in such an extent that they become inferior to the threshold values separating the pharmaceutical and cosmetic fields, and this without lowering the efficiency of the product by this way. 
     A substance or a composition according to the invention can also be used in products which are neither cosmetics nor pharmaceutical products, for example as antifungal, antiprotozoal, antiseptic, antibiotic or other in the field of agriculture, or also in the field of the disinfection of surfaces. 
    
    
     PREFERRED EMBODIMENTS OF THE INVENTION 
     The preferred embodiment of the invention presently known is the one of the products locally used on the genitals of male and/or female mammals, as spermicides and/or bactericides for fighting against sexually transmissible diseases (STD). 
     Benzalkonium chloride and nonoxynol 9 are preferably used as active ingredient. Any metallic derivative of fluorine, e.g. sodium fluoride is preferably used as activating ingredient. All the known galenical forms are usable, such as preferably ovule, cream, gel, solution, foam, tablet, soluble waffle, tampon, vaginal suppository or others. 
     The proportions to be used of active and activating ingredients depends on the galenical form since only the actual concentrations induced in vivo are important as regards to the efficiency of the product. 
     These proportions must vary between the MIC and the maximal concentrations from which side effects are induced. In local administration, intolerances (irritations) of the treated parts must be avoided. In general administration, toxic concentrations must be avoided. 
     Thus, the Benzalkonium Chloride concentration in vivo is preferably 1.2% (in weight) and the fluoride anion F -  concentration in vivo is preferably 0.5% (in weight). 
     Several preferred embodiments of the invention are hereafter described, in several tested uses, by referring to tests carried out on several unicellular living creatures. 
     (I) USE OF THE INVENTION FOR LOCAL CONTRACEPTION 
     The invention advantageously provides a spermicidal pharmaceutical product locally applied so that it comes into contact with sperm and kills or inhibits the spermatozoa. 
     A spermicidal pharmaceutical product according to the invention may be presented under various galenical forms: tablet, ovule, soluble, cream, gel, soluble waffle, tampon, foam, vaginal suppository, inserted within the vagina before sexual intercourse for preventing a fertilization by killing or inhibiting the spermatozoa before they come into contact with the ovula. 
     A product according to the invention comprises a composition which includes at least one chemical compound according to the invention which includes ionizable fluorine, i.e. capable of emitting fluoride anion F - , as an active ingredient spermicidal directly or by potentiation. 
     A chemical compound according to the invention is capable of emitting fluoride anion F -  when soluted, in particular in aqueous solution. For example, it is constituted of a metallic derivative of fluorine such as sodium fluoride, calcium fluoride, potassium fluoride, aluminium fluoride, tin fluoride, ammonium fluoride, sodium monofluorophosphate . . . , or an organic fluorinated compound such as a fluorinated amine. 
     For example, for measuring the spermicide activity of a chemical compound, one may use the total spermicidal test according to the standards of IPPF, which consists in finding the minimal inhibiting concentration called MIC (expressed with a weight percentage) of the chemical compound in one milliliter of solution inducing the death of all the spermatozoa which are contained in 0.2 milliliter of sperm in less than 5 seconds. The test is carried out on at least 6 sperms from various donors, satisfying the following minimal conditions from IPPF: 
     age of the sample: two hours; 
     density per mm 3  : 50 million spermatozoa; 
     mobility: 50% of the spermatozoa should move forward fastly when examined at 35°-37° C. in a recent sample; 
     viscosity: ejaculum conveniently liquefied, not filaceous and homogeneous-looking with the naked eye; 
     collected in sterile glass tubes hermetically closed, preserved at 37° C. 
     The inventors have found that, under these conditions, fluoride anion F -  has a spermicide activity of 100% according to the IPPF test when present at a titer of 5 ppm (e.g. 5 milligrams per liter). 
     A pharmaceutical spermicidal composition according to a first variant of the invention includes at least one fluorinated chemical compound capable of emitting fluoride anion F -  as unique active inhibiting or destroying ingredient. The titer of F -  in the composition is advantageously superior to 4.5 ppm, preferably about 5 ppm when a 100% inhibiting composition is desired. 
     Moreover, the inventors have found that fluoride anion F - , in addition to this direct spermicide activity, has a spermicide activity by potentiation of known spermicidal compounds. Therefore, a composition according to the invention is advantageously constituted on one hand of at least one basic active spermicidal ingredient such as a cationic, or anionic, or amphoteric or non-ionic surfactant lowering the interfacial tension, or even para-methanylphenylpolyoxyethylene ether or a trisodium salt of polysaccharide sulfuric ether or other, on second hand of at least one fluorinated compound according to the invention capable of emitting fluoride anion F - , and finally of a pharmaceutical excipient and various common additives (anti-oxydant, . . . ). 
     In a composition according to the invention, the basic active ingredient concentration may be inferior to the MIC of this basic active ingredient without ion F - , the composition being nevertheless 100% inhibiting, i.e. satisfying the total spermicidal test of IPPF. A composition according to the invention may include an admixture of several basic active ingredients inhibiting gametes and/or an admixture of several various fluorinated chemical compound. 
     Hereafter, the percentages are given in weight. 
     The preferred embodiments of the invention for its uses in local contraception are as follows: 
     
         ______________________________________OVULES:______________________________________benzalkonium chloride             1.20%anion F           0.50%(e.g. sodium fluoride)______________________________________ 
    
     Excipients: semi-synthetic glycerides or cacao butter, or gelatin or glycerin and purified water, antioxygens, antiseptics. 
     
         ______________________________________CREAMS AND MILKS:______________________________________benzalkonium chloride             1.20%anion F           0.50%(e.g. sodium fluoride)______________________________________ 
    
     Excipients: distilled or purified water, humectants, emulsifiers, stabilizers, antioxygens, antiseptics (to be included in variable proportions according to the viscosity and pH to be obtained). 
     
         ______________________________________OINTMENTS AND POMMADES:______________________________________benzalkonium chloride             1.20%anion F           0.50%(e.g. sodium fluoride)______________________________________ 
    
     Excipients: distilled or purified water, emulsifier, excipients of the kind of the fatty compounds vaseline petroleum jelly, lanoleine, lanovaseline, stearovaseline), stabilizers, antioxygens, antiseptics (to be included in variable proportions according to the viscosity and pH to be obtained). 
     
         ______________________________________GEL:______________________________________benzalkonium chloride             1.20%anion F           0.50%(e.g. sodium fluoride)______________________________________ 
    
     Excipients: soluble derivatives of cellulose compatible with the cationic surfactants, distilled or purified water, glycerin, sorbitol, antioxygens, antiseptics (to be included in variable proportions according to the viscosity and pH to be obtained). 
     
         ______________________________________SOLUBLE WAFFLE:______________________________________benzalkonium chloride             1.20%Anion F           0.50%(e.g. sodium fluoride)______________________________________ 
    
     Excipients: polyvinyl alcohol, glycerin, sorbitol, propylene glycol, distilled or purified water, antioxygens. 
     
         ______________________________________TABLETS:______________________________________benzalkonium chloride            25 mg per tabletanion F          10 mg per tablet(e.g. sodium fluoride)______________________________________ 
    
     Excipients: lactose, magnesium stearate, cellulose, amylum, citric acid, sodium bicarbonate. 
     
         ______________________________________SYNTHETIC SOAPS:______________________________________benzalkonium chloride              2%anion F            1%(e.g. sodium fluoride)______________________________________ 
    
     Excipients: foaming and wetting products compatible with quaternary ammonia (e.g. amphoteric surfactant such as betaine or amino-betaine) emollients, stabilizers, antioxygens, antiseptics. 
     
         ______________________________________SOLUTIONS:______________________________________benzalkonium chloride             0.50%anion F           0.25%(e.g. sodium fluoride)______________________________________ 
    
     Excipients: distilled or purified water, ethanol, antioxygens, glycerin, sorbitol, antiseptics (to be included in variable proportions according to the pH to be obtained). 
     COMPARATIVE TEST NO. 1 
     The MIC of various known spermicidal compounds were determined with the IPPF test. The following results have been obtained by 1 milliliter of composition reacting with 0.2 milliliter of sperm and by determining the minimal concentration of the spermicidal compounds that induces the death of all the spermatozoa in 5  seconds, and this with 6 sperms from various donors. 
     
         ______________________________________Spermicidal compound   MIC (% in weight)______________________________________p-menthanylphenylpolyoxyethylene ether                  0.006Trisodium salt of polysaccharide                  0.007sulfuric etherAnionic surfactant     1(methyltauride sodium oxysalt)Cationic surfactant    0.006(benzalkonium chloride)Non-ionic surfactant   0.006(nonoxynol 9)Amphoteric surfactant  0.001(derivative of amidoethylglycinateon fatty acids)______________________________________ 
    
     TEST NO. 2 
     The same total spermicidal test of IPPF was carried out with 1 milliliter of composition already containing 0.0001% (in weight) of fluoride anion F -  reacting upon 0.2 milliliter of sperm, by determining the minimal concentration of the spermicidal compounds that induces the death of all the spermatozoa in 5 seconds, and this with 6 sperms from various donors. 
     
         ______________________________________Spermicidal compound   MIC (% in weight)______________________________________p-menthanylphenylpolyoxyethylene ether                  0.003Trisodium salt of polysaccharide                  0.0025sulfuric etherAnionic surfactant     0.50(methyltauride sodium oxysalt)Cationic surfactant    0.002(benzalkonium chloride)Non-ionic surfactant   0.003(nonoxynol 9)Amphoteric surfactant  0.0005(derivative of amidoethylglycinateon fatty acids)______________________________________ 
    
     It is thus noticeable that the inhibiting ability of the known spermicidal ingredients is much improved with the adjunction of ionizable fluorine in the composition. A composition according to the invention may therefore contain very little basic active spermicidal ingredient, in particular in a concentration inferior to the minimal inhibiting concentration of this active ingredient without ion F - . 
     Moreover, the concentration of ion F -  associated to a basic active ingredient may also be very low, in particular inferior to the minimal inhibiting concentration of fluoride anion F - . 
     As an example, the inventors have found that when a solution containing 0.003% of benzalkonium chloride and a solution containing 0.0001% of fluoride anion F -  each do not have a total spermicide activity according to the IPPF test, a solution containing 0.003% benzalkonium chloride and 0.001% of fluoride anion F -  both together does satisfy the IPPF test. A synergistic effect is therefore observed. 
     TEST NO. 3 
     This test consists in realizing a test identical to tests No. 1 and 2 above mentioned, but in presence of sodium borate, in order to complex ion F -  : the spermicidal effect of ion F -  has totally disappeared, thus demonstrating that only ion F -  is active or potentiator of the spermicidal effect. 
     TEST NO. 4 
     Under the same conditions previously described (Tests No. 1 to 3), various perfluorinated benzalkonium chlorides were used as basic spermicidal compounds. It was thus shown that perfluorinated benzalkonium chlorides have a spermicide activity similar to the one of the mere benzalkonium chloride. Besides, this activity remains identical to itself under the conditions of test No. 3, in presence of sodium borate, which demonstrates that fluorine fixed on the benzine nucleus was not ionized. 
     Test No. 2 was also realized on perfluorinated benzalkonium chloride mixed with another chemical compound capable of emitting ion F - . The potentiation of the spermicide activity of the perfluorinated benzalkonium chloride was also observed. This same test realized in presence of sodium borate (according to test No. 3) did induce a spermicide activity which is the one of perfluorinated benzalkonium chloride. 
     These two tests No. 3 and 4 show that the potentiation of the basic active ingredient only takes place in presence of fluoride anion F - . 
     For manufacturing a spermicidal pharmaceutical product according to the invention, a solution with a given concentration in fluoride anion F -  and, eventually, in basic active ingredient is realized and integrated in the excipient chosen according to the galenical form which is intended to be manufactured. In the case of the tablet, the basic active ingredient and the chemical compound capable of emitting ion F -  are integrated to the excipient in the form of primary products. 
     COMPARATIVE TEST NO. 5 
     This test in vitro was conducted on galenical preparations including benzalkonium chloride as basic active ingredient, by determining the MIC of this benzalkonium chloride after simulation in vitro of the actual conditions in vivo (extraction, solubilization . . . ). 
     This test conducted without activating ingredient induced the following results: 
     
         ______________________________________         MIC (% in weight)         of benzalkoniumGalenical form         chloride______________________________________ovule         0.0063Cream         0.0083Tampon        0.0075Tablet        0.0095Soluble waffle         0.0080Gel           0.0080______________________________________ 
    
     The percentages correspond to the proportions (in weight) of benzalkonium chloride in the solution used in vitro for the spermicidal test according to IPPF and obtained after simulation, the proportions being measured by titrating using a sample taken off from the solution. 
     TEST NO. 6 
     The same conditions as during the comparative test No. 5 were used, but from galenical forms each originally containing 0.45% (in weight) of fluoride anion F - . 
     The following results were obtained: 
     
         ______________________________________         MIC (% in weight)         of benzalkoniumGalenical form         chloride______________________________________ovule         0.0023Cream         0.0030Tampon        0.0025Tablet        0.0025Soluble waffle         0.0017Gel           0.0033______________________________________ 
    
     (II) USE OF THE INVENTION FOR ANTISEPSIS, ANTIBIOTICS, . . . AND IN PARTICULAR FOR FIGHTING AGAINST THE STD 
     The preferred embodiments of the invention for its use, e.g. as antiseptic in dermatology are the following ones: 
     
         ______________________________________CREAMS AND MILKS:______________________________________benzalkonium chloride             1.20%anion F           0.50%(e.g. sodium fluoride)______________________________________ 
    
     Excipients: distilled or purified water, humectants, emulsifiers, stabilizers, antioxygens, antiseptics (to be included in variable proportions according to the viscosity and pH to be obtained). 
     
         ______________________________________OINTMENTS AND POMMADES:______________________________________benzalkonium chloride             1.20%anion F           0.50%(e.g. sodium fluoride)______________________________________ 
    
     Excipients: distilled or purified water, emulsifiers, excipients of the kind of the fatty compounds (vaseline, lanoleine, lanovaseline, stearovaseline), stabilizers, antioxygens, antiseptics (to be included in variable proportions according to the viscosity and pH to be obtained). 
     
         ______________________________________SYNTHETIC SOAPS:______________________________________benzalkonium chloride              2%anion F            1%(e.g. sodium fluoride)______________________________________ 
    
     Excipients: foaming and wetting products compatible with quaternary ammonia (e.g. amphoteric surfactant such as betaine or amino-betaine) emollients, stabilizers, antioxygens, antiseptics. 
     
         ______________________________________SOLUTIONS______________________________________benzalkonium chloride   0.50%anion F.sup.-           0.25%(e.g. sodium fluoride)______________________________________ 
    
     Excipients: distilled or purified water, ethanol, antioxygens, glycerine, sorbitol, antiseptics (to be included in variable proportions according to the pH to be obtained). 
     The preferred embodiments of the invention for its use, e.g. as local antibiotic in dermatology are the following ones: 
     
         ______________________________________SOLUTION______________________________________erythromycine base     4%anion F.sup.-          0.5%______________________________________ 
    
     Excipients: ethyl alcohol, propylene glycol, distilled water. 
     
         ______________________________________GEL______________________________________erythromycine base     4%anion F.sup.-          0.5%______________________________________ 
    
     Excipients: ethyl alcohol, hydroxypropylcellulose, distilled water, glycerine. 
     
         ______________________________________POMMADES______________________________________neomycin base          0.35%anion F.sup.-          0.50%orbacitracin             50.000 IU %anion F.sup.-          0.50%oroxytetracycline chlorhydrate                  3%anion F.sup.-          0.50%oraureomycin chlorhydrate                  3%anion F.sup.-          0.50%______________________________________ 
    
     Excipients: vaseline, vaseline oil, lanoline. 
     
         ______________________________________CREAMS______________________________________soframycin sulfate     2.5%anion F.sup.-          0.50%______________________________________ 
    
     Excipients: propylene glycol, polyoxyethyleneglycol, distilled water. 
     The preferred embodiments of the invention for its use, e.g. as local antibiotic in otorhinolaryngology are the following ones: 
     
         ______________________________________OPHTALMIC POMMADES______________________________________aureomycin               1%anion F.sup.-            0.50%oroxytetracycline          0.50%anion F.sup.-            0.25%______________________________________ 
    
     Excipients: vaseline, vaseline oil, lanoline 
     
         ______________________________________NASAL SOLUTIONS______________________________________soframycin sulfate      1.25%anion F.sup.-           0.50%______________________________________ 
    
     Excipients: distilled water, citric acid, sodium chloride. 
     For increasing the activity of the local antibiotherapeutical preparations, 0.10% of benzalkonium chloride may advantageously be added. 
     The preferred embodiments of the invention for its use, e.g. in gynecology and more particularly for fighting against the sexually transmissible diseases STD are those described under the item &#34;USE OF THE INVENTION FOR LOCAL CONTRACEPTION&#34; and under the denominations &#34;OVULES&#34;, &#34;CREAMS&#34;, &#34;OINTMENTS AND POMMADES&#34;, &#34;GELS&#34;, &#34;TABLETS&#34;, &#34;SYNTHETIC SOAPS&#34; and &#34;SOLUTIONS&#34;. 
     The methodology used in the tests conducted for those uses of the invention was as follows: 
     For Neisseria gonorrhoeae: 
     The used germs come from hospital isolations 
     dilution range of the substances to be tested: 
     Solvent: bidistilled sterile H 2  O 
     Range with geometrical ratio of 2 
     Doses: from 2000 to 1.56 (and less) μg/ml 
     2 ml of each of the dilutions are mixed with 18 ml of solid preferable culture medium: gelose medium for isolation of gonococci (Institut Pasteur) enriched with G supplement: 
     
         ______________________________________Formula:    Poulain serum  165      ml       yeast extract  100      ml       globular extract                      235      ml       glucose        0.65     ml______________________________________ 
    
     Culture medium of the gonococci: 2000 ml of Pasteur medium+500 ml of G supplement. 
     Those culture medium associated to the dilutions of the active substances form the investigation means of the bactericide activity. The final concentrations of the substances are about from 800 to 0.156 μg/ml in the Petri dishes. The used suspensions (dilution in physiological water of a 24 hours-culture in nutritive liquid-broth medium) are placed in wells hollowed in a synthetic base under laminar-flow hood. In each of the prepared wells, a strain (multiple seeder) is studied. Each studied dish is examined after 24 and 48 hours of culture (stove at 37° C.). 
     Determination of the MIC: the gonococci strains are spreaded on plates (dilution on plate) some of them being prepared as comparative and control strains without substance to be tested. 
     5 μl of 10 formative colonies/ml are deposited on the gelose plates completed or not with the substances to be tested (18 hours culture of gonococci suspended in a Sorensen solution). 
     A 48 h incubation at 36° C. is carried out, then the growth or the lack of growth of the gonococci is observed on the plates. 
     For Candida albicans: 
     The methodology is substantially the same as for Neisseria gonorrhoeae, but with the following differences: 
     Dilution range in bidistilled sterile water from 2000 to 1.56 μg/ml. 
     0.5 ml of each dilution is added to 4.5 ml of liquid Roiron medium. 
     The tubes are seeded with cultures of Candida (24 hours/Roiron medium) or with the comparative strain (Roiron medium). The incubation of the tubes is conducted in stove at 37° C. during 24 and 48 hours. At t=24 hours and t=48 hours, observation of a drop between slide and cover-glass under microscope. 
     Expression of the results by accounting the yeasts and determination of the fungicide MIC. On comparative tubes, determination of the percentage in resistants. 
     For Trichomonas vaginalis: 
     Same methodology as for Candida albicans. 
     For Chlamydia: 
     Inoculum: it is constituted by the host-cells of chlamydia or Mac Coy cells. They are preserved at -80° C. The cellular concentration is from 2 to 2.5×10 cells/ml for the tests. The cellular layer is prepared in a complete culture medium. 
     The substances to be tested are diluted with an increasing range which geometrical ratio is 2 in bidistilled sterile water. 
     The used method is the one of Professors F. CATALAN, P. SEDNAOUI, A. MILOVANOVIC and al., A. FOURNIER INSTITUTE, Paris. 
     1 ml of dilution of the substances to be tested is mixed with 1 ml of cellular suspension (Mac Coy cells), then incubated 1 hour and 24 hours at 37° C. in stove. 0.2 ml of this admixture is then placed in the plates of the wells. The plates are centrifugated (1 hour at 2000 rpm) and incubated 1 hour at 37° C. The culture medium is then replaced with 0.2 ml of new medium containing 0.5 mg/l of cycloheximide and the plates are then incubated 48 hours at 37° C. The cellular layer is then fixed with methanol and overall couloured with a monoclonal antibody and conjugate with FITC (fluorescein isothiocyanate). Then the inclusions present in the Mac Coy cells are observed with an inversed epifluorescence microscope. 
     Therefore, the toxicity of the substances to be tested was first tested, and was taken into account for totally respecting the integrity of the host-cell. 
     For Pseudominas aeruginosa: 
     Preparation of a dense solution with two strains. 
     Suspension in isotonic solution of NaCl (0.85% in weight). 
     Checking that the suspensions include the same number of bacteria: regular techniques in gelose medium by grooved seeding (1 μl ansa) of the successive dilutions of the original dense solutions (geometrical ratio 10). Incubation 18 hours at 37° C. 
     Preparation of the dilutions of the substances to be tested in solution in bidistilled sterile water. Range of dilution with geometrical ratio of 2 (e.g. from 2000 to 0.015 μg/ml). 
     Admixture of the Pseudominas Aeruginosa bacterial suspension (same origin as the AFNOR standards), with a constant concentration (=4×10 7  bacteria/ml) and with the dilution of the substances in a decreasing order (2000 to 0.015 μg/ml). 
     Incubation or contact time: 1 hour, 24 hours and 48 hours in stove at 37° C. 
     The tests were doubled for each dilution of substance. 
     Observation of the number of survivors recorded with regard to the average number of the comparative strains cultured simultaneously with the tests. 
     Observation after 1 hour, 24 hours and 48 hours. 
     For Treponema, Gardnerella, Ducrey&#39;s Bacillas, Streptococci and Staphylococcus aureus: 
     The methodology used is the one described by Professors F. CATALAN, P. SEDNAOUI, A. MILOVANOVIC and al., A. FOURNIER INSTITUTE. 
     COMPARATIVE TEST NO. 7 
     With benzalkonium chloride alone and nonoxynol 9 alone, the following results were obtained: 
     
         ______________________________________          Minimal inhibitinq concentration          (MIC)STRAINS        Benzalkonium chloride alone______________________________________Gonococcus     1.15         mg/lTreponema      70           mg/lTrichomonas    1.3          mg/lCandidas Albicans          50           mg/lChlamydia      100          mg/lGardnerella    50           mg/lDucrey&#39;s Bacillus          75           mg/lStreptococcus  15           mg/lPseudomonas Aeruginosa          31.25        mg/lStaphylococcus Aureus          1.56         mg/l______________________________________          Minimal inhibiting concentration          (MIC)STRAINS        Nonoxynol 9 alone______________________________________Treponema      75           mg/lPseudomonas Aeruginosa          50           mg/lStaphylococcus Aureus          4            mg/lStreptococcus  20           mg/l______________________________________ 
    
     TEST NO. 8 
     The same test as precedently, conducted in presence of fluoride anion F -  gave the following results: 
     
         ______________________________________          Minimal inhibiting concentration          (MIC)          Benzalkonium chloride + F.sup.-STRAINS        1 microgram/ml______________________________________Gonococcus     0.60         mg/lTreponema      56           mg/lTrichomonas    0.9          mg/lCandidas Albicans          35           mg/lChlamydia      85           mg/lGardnerella    41           mg/lDucrey&#39;s Bacillus          62           mg/lStreptococcus  9            mg/lPseudomonas Aeruginosa          18           mg/lStaphylococcus Aureus          1.1          mg/l______________________________________          Minimal inhibiting concentration          (MIC)          Nonoxynol 9 + F.sup.-STRAINS        1 microgram/ml______________________________________Treponema      60           mg/lPseudomonas Aeruginosa          35           mg/lStaphylococcus Aureus          2.5          mg/lStreptococcus  15           mg/l______________________________________ 
    
     COMPARATIVE TEST NO. 9 
     This test was conducted with a substance including benzalkonium chloride without activating ingredient but in presence of serous proteins. It gave the following results: 
     
         ______________________________________                     Minimal inhibiting    CONCENTRATIONS   concentration (MIC)    IN SEROUS        BenzalkoniumSTRAINS  PROTEINS         chloride alone______________________________________Streptococcus    0                15       mg/mlFaecalis    30 mg/ml         15       mg/ml    60 mg/ml         16       mg/ml    90 mg/ml         68       mg/mlNeisseria    0                1.5      mg/mlGonorrhoeae    30 mg/ml         1.5      mg/ml    60 mg/ml         1.5      mg/ml    90 mg/ml         9        mg/ml______________________________________ 
    
     This test shows the known unfavourable effect of the serous proteins upon the efficiency of benzalkonium chloride. 
     TEST NO. 10 
     This test was conducted identically with test No. 9, but in presence of fluoride anion F -  in the composition. The following results were obtained. 
     
         ______________________________________                    Minimal inhibitinq    CONCENTRATIONS  concentration (MIC)    IN SEROUS       Benzalkonium chlorideSTRAINS  PROTEINS        + F.sup.-  1 microgram/ml______________________________________Streptococcus    0               9         mg/mlFaecalis    30 mg/ml        9         mg/ml    60 mg/ml        9         mg/ml    90 mg/ml        21        mg/mlNeisseria    0               0.6       mg/mlGonorrhoeae    30 mg/ml        0.6       mg/ml    60 mg/ml        0.6       mg/ml    90 mg/ml        1.2       mg/ml______________________________________ 
    
     This test shows that fluoride anion F -  advantageously decreases the unfavourable effect of the serous proteins. 
     COMPARATIVE TEST NO. 11 
     This test was conducted on galenical forms in vitro (similarly to tests No. 5 et 6), with products containing benzalkonium chloride as active ingredient and free of activating ingredient. 
     The following results were obtained: 
     
         ______________________________________                  Minimal inhibiting                  Concentration (MIC)STRAINS  GALENIC FORM  Benzalkonium chloride alone______________________________________Gonococcus    ovule         23.65 mg/l    tablet        15.62 mg/lTrichomonas    ovule         11.80 mg/l    tablet        15.60 mg/l______________________________________ 
    
     The concentrations are those present in the liquid obtained after simulation, used for the test. 
     TEST NO. 12 
     This test was conducted in the same conditions as the comparative test No. 11 from products containing 0.5% (in weight) of fluoride anion F - . 
     The following results were obtained: 
     
         ______________________________________                    Minimal inhibiting                    Concentration (MIC)                    Benzalkonium chlorideSTRAINS   GALENIC FORM   + 0.5% F.sup.-______________________________________Gonococcus     ovule          5 mg/l     tablet         3 mg/lTrichomonas     ovule          5 mg/l     tablet         3 mg/l______________________________________ 
    
     (III) USE OF THE INVENTION IN THE FIELD OF DISINFECTION 
     This field relates to the treatment of floors, surfaces, instruments . . . with contact bactericidal products. 
     The preferred embodiments of the invention for this use are as follows: 
     
         ______________________________________       Benzalkonium                   AnionUTILIZATIONS       Chloride    F.sup.-  Excipients______________________________________Hands       0.1%        0.25%    purified waterEpidermis                        or alcohol                            to make up                            for 100%Instruments 1.0%        0.50%    purified waterto be sterilized                 to make upto be disinfected                for 100%Textiles     0.05%       0.025%  purified water                            to make up                            for 100%Instruments 1.0%        0.50%    Ethanol 10%(e.g. thermometer)               purified water                            to make up                            for 100%Cleaning of 0.1%        0.25%    purified watersurfaces (rooms,                 to make upfloor . . .)                     for 100%______________________________________ 
    
     The tests were conducted according to the standard AFNOR NFT 72-150, Mar. 81, with benzalkonium chloride, then with nonoxynol 9 as active ingredient. 
     The neutralizer used was as follows: 3% Tween 80 (V/V) and 0.3% lecithin (M/V). The pH of the medium was 7.2. 
     COMPARATIVE TEST NO. 13 
     This test was conducted without activating ingredient: 
     
         ______________________________________            Minimal inhibiting            Concentration (MIC)STRAINS          Benzalkonium chloride alone______________________________________Pseudomonas aeruginosa            31.25       mg/lCNCM A 22Escherichia coli 6.57        mg/lCNCM 54 127Staphylococcus Aureus            1.56        mg/lOxford origin CNCM 53 154Streptococcus faecalis            4           mg/lCNCM 5 855Mycobacterium smegmatis            30          mg/lCNCM 7 326______________________________________            Minimal inhibiting            Concentration (MIC)STRAINS          Nonoxynol 9 alone______________________________________Pseudomonas aeruginosa            50          mg/lCNCM A 22Escherichia coli 8           mg/lCNCM 54 127Staphylococcus Aureus            4           mg/lOxford origin CNCM 53 154            4           mg/lStreptococcus faecalis            7           mg/lCNCM 5 855Mycobacterium smegmatis            65          mg/lCNCM 7 326______________________________________ 
    
     TEST NO. 14 
     This test is identical to the previous one but in presence of fluoride anion F -  as activating ingredient. 
     
         ______________________________________            Minimal inhibiting            Concentration (MIC)            Benzalkonium chloride + F.sup.-STRAINS          1 microgram/ml______________________________________Pseudomonas aeruginosa            18          mg/lCNCM A 22Escherichia coli 3           mg/lCNCM 54 127Staphylococcus Aureus            1.1         mg/lOxford origin CNCM 53 154Streptococcus faecalis            3.6         mg/lCNCM 5 855Mycobacterium smegmatis            26          mg/lCNCM 7 326______________________________________            Minimal inhibiting            Concentration (MIC)            Nonoxynol 9 + F.sup.-STRAINS          1 microgram/ml______________________________________Pseudomonas aeruginosa            35          mg/lCNCM A 22Escherichia coli 6.5         mg/lCNCM 54 127Staphylococcus Aureus            2.5         mg/lOxford origin CNCM 53 154Streptococcus faecalis            5.5         mg/lCNCM 5 855Mycobacterium smegmatis            50          mg/lCNCM 7 326______________________________________ 
    
     (IV) USE OF THE INVENTION IN COSMETOLOGY 
     The preferred embodiments of the invention in cosmetology are described hereinafter: 
     The following galenical forms may be presented in cosmetology: creams, milks, pommades, solutions, foaming bath, synthetic soaps, shampoos, intimate lotions, disinfecting lotions. 
     The formula of the excipients are the same as for the pharmaceutical preparations but the concentrations in benzalkonium chloride and anion F -  are different. Those concentrations are preferably as follows for all the products: 0.2% benzalkonium chloride and 0.1% fluoride anion F - . 
     Chemical or naturally originated active ingredients may also enter into these formulae with concentrations and doses which are allowed in cosmetology. 
     The creams and milks may be in continuous aqueous phase (oil/water or water/oil emulsion) or pasty when warm, self diluting in water. 
     The various excipients referred to as examples correspond in a non-restrictive and indicative way to the following products: 
     Humectants: glycerol, propylene glycol, diethylene glycol, glycol, sorbitol, polyoxyethylene glycol. 
     Emulsifiers: sodium stearate, beeswax, sorbitolester, polyoxyethylene glycol ester, fatty alcohol, triethanolamine lanoline, tween, glycol stearate and polyglycols. 
     Stabilizers: glycol stearate, cetylic alginate alcohol, pectin, gum, fatty esters of polyols, soluble cellulose esters. 
     Antioxygens: tartaric acid, citric and ascorbic acid. 
     Antiseptic: boric acid, benzoic acid, parabenzoic acid, and their methylic or propylic esters, soded or not. 
     PH: all these formulas are particularly efficient with pH comprised between 4.5 and 6.5. For obtaining such a range, citric acid is mainly used.