Abstract:
One embodiment of the present invention provides a sweetener composition, which includes N-{N-(3,3-dimethylbutyl)-L-α-aspartyl}-L-phenylalanine 1-methyl ester, and Aspartame, wherein a ratio of the Aspartame to a total amount of the Aspartame and the N-{N-(3,3-dimethylbutyl)-L-α-aspartyl}-L-phenylalanine 1-methyl ester is in the range of 10 to 99.5% by weight, methods of making and of using. Another embodiment of the present invention provides a method for preparing a sweetener composition, which includes drying an A-type crystal of N-{N-(3,3-dimethylbutyl)-L-α-aspartyl }-L-phenylalanine 1-methyl ester to obtain a C-type crystal of N-{N-(3,3-dimethylbutyl)-L-α-aspartyl }-L-phenylalanine 1-methyl ester. Another embodiment of the present invention provides a method for producing a sweetener, which includes admixing N-{N-(3,3-dimethylbutyl)-L-α-aspartyl }-L-phenylalanine 1-methyl ester with Aspartame, wherein a ratio of the Aspartame to a total amount of the N-{N-(3,3-dimethylbutyl)-L-α-aspartyl }-L-phenylalanine 1-methyl ester and the Aspartame is in the range of 10 to 99.5% by weight. Another embodiment of the present invention provides a method for improving the dissolution rate of N-{N-(3,3-dimethylbutyl)-L-α-aspartyl }-L-phenylalanine 1-methyl ester, which includes, prior to dissolving the N-{N-(3,3-dimethylbutyl)-L-α-aspartyl }-L-phenylalanine 1-methyl ester, admixing the N-{N-(3,3-dimethylbutyl)-L-α-aspartyl }-L-phenylalanine 1-methyl ester with Aspartame, wherein a ratio of the Aspartame to a total amount of the N-{N-(3,3-dimethylbutyl)-Lα-aspartyl}-L-phenylalanine 1-methyl ester and the Aspartame is in the range of 10 to 99.5% by weight.

Description:
This application is a continuation of PCT/JP99/02198, which was filed Apr. 26, 1999. 
    
    
     BACKGROUND OF THE INVENTION 
     1. Field of the Invention 
     The present invention relates to a sweetener composition having excellent solubility, which includes N-{N-(3,3 -dimethylbutyl)-L-α-(aspartyl}-L-phenylalanine 1-methyl ester (Neotame, abbreviated hereinafter to “NM”) and Aspartame (abbreviated hereinafter to “APM”) as active ingredients. 
     2. Discussion of the Background 
     It is reported that the sweetness strength or sweetening potency of the synthetic high-potency sweetener, NM, is about 10,000 times that of sucrose in terms of weight ratio (Japanese Patent Kohyou Publication JP-A-8-503206). The properties of sweetness quality for NM are not reported in detail, but the present inventors have found that such a compound has an extremely weak early taste (i.e., wherein the sweetener, when put in the mouth, tastes sweet as early as sucrose), and is extremely strong in later taste (i.e., wherein the sweetener tastes sweet later than sucrose). Further, NM has a strong astringent taste. Accordingly, the balance of the quality of sweetness properties for NM is poor when compared to sucrose. Sucrose is generally regarded as the standard for evaluating the properties or characteristics of the quality of sweetness. 
     It is also reported that the sweetness strength of the amino acid type synthetic sweetener, APM, is about 200 times that of sucrose in weight ratio (See Japanese Patent Kokoku Publication JP-B-47-3 1031). APM has a sweetness quality characterized by a weak early taste and a strong later taste as compared with sucrose. 
     Various proposals have been made for the improvement in quality of the sweetness of NM and APM, particularly for the improvement in quality of the sweetness of the latter, thus achieving considerable effects. However, NM and APM have a further problem with dissolution characteristics; that is, NM and APM powders (crystalline raw powders) have poor dissolution characteristics in water (that is, they are not readily dissolved due to their easy formation of agglomerates, or their dissolution rate is low, etc.). Poor dissolution characteristics, which may result from the formation of agglomerates, or the like is significantly disadvantageous to industrial production, since the production yield of foods and drinks such as soft drinks that contain NM or APM to confer sweetness is undesirably reduced. 
     For improvement of the dissolution rate of APM, various proposals using pelletizing methods (granulations) have been made. However, these methods are not satisfactory in that they require further improvement of dissolution rate (See Japanese Patent Kokai Publication JP-A-4-346769 etc.) and require the simultaneous use of a relatively large amounts of excipients (See Japanese Patent Kokai Publications JP-A-49-126855, JP-A-50-19965, JP-A-57150361 etc.). 
     SUMMARY OF THE INVENTION 
     Accordingly, one object of the present invention is thus to improve the dissolution rate of NM and APM. 
     The present inventors have unexpectedly found that the dissolution rate of NM, and particularly that of a specific crystal of NM (which may be also called “C-type crystal”) is improved by APM in a certain range, and vice versa, and further that the dissolution rate of a mixture of both of them at a specific mixing range is higher than not only that of NM alone but also that of APM alone, and this phenomenon is particularly remarkable and significant when NM is in the form of C-type crystal. 
     Accordingly, one embodiment of the present invention provides a sweetener composition, which includes: 
     N-{N-(3,3-dimethylbutyl)-L-α-aspartyl}-L-phenylalanine 1-methyl ester; and 
     Aspartame, wherein a ratio of the Aspartame to a total amount of the Aspartame and the N-{N-(3,3 -dimethylbutyl)-L-α-aspartyl}-L-phenylalanine 1-methyl ester is in the range of 10 to 99.5% by weight. 
     Another embodiment of the present invention provides a drink composition, which includes: 
     a mixture of N-{N-(3,3-dimethylbutyl)-L-α-aspartyl}-L-phenylalanine 1-methyl ester; 
     Aspartame; and 
     a potable liquid, wherein 
     a ratio of the Aspartame to a total amount of the N-{N-(3,3-dimethylbutyl)-L-α-aspartyl}-L-phenylalanine 1-methyl ester and the Aspartame is in the range of 10 to 99.5% by weight. 
     Another embodiment of the present invention provides a method for preparing a sweetener composition, which includes: 
     drying an A-type crystal of N-{N-(3,3 -dimethylbutyl)-L-α-aspartyl}-L-phenylalanine 1-methyl ester to obtain a C-type crystal of N-{N-(3,3-dimethylbutyl)-L-α-aspartyl}-L-phenylalanine 1-methyl ester. 
     Another embodiment of the present invention provides a method for producing a sweetener, which includes: 
     admixing N-{N-(3 ,3-dimethylbutyl)-L-α-aspartyl}-L-phenylalanine 1-methyl ester with Aspartame, wherein a ratio of the Aspartame to a total amount of the N-{N-(3,3-dimethylbutyl)-L-α-aspartyl}-L-phenylalanine 1-methyl ester and the Aspartame is in the range of 10 to 99.5% by weight. 
     Another embodiment of the present invention provides a method for improving the dissolution rate of N-{N-(3,3-dimethylbutyl)-L-α-aspartyl}-L-phenylalanine 1-methyl ester, which includes, prior to dissolving the N-{N-(3,3-dimethylbutyl)-L-α-aspartyl }-L-phenylalanine 1-methyl ester, admixing the N-{N-(3,3-dimethylbutyl)-L-α-aspartyl }-L-phenylalanine 1-methyl ester with Aspartame, wherein a ratio of the Aspartame to a total amount of the N-{N-(3,3-dimethylbutyl)-L-α-aspartyl}-L-phenylalanine 1-methyl ester and the Aspartame is in the range of 10 to 99.5% by weight. 
    
    
     BRIEF DESCRIPTION OF THE FIGURES 
     A more complete appreciation of the invention and many of the attendant advantages thereof will be readily obtained as the same becomes better understood by reference to the following detailed description when considered in connection with the accompanying drawings, wherein: 
     FIG.  1 : A powder X-ray diffraction pattern of A-type crystals. 
     FIG.  2 : A powder X-ray diffraction pattern of C-type crystals. 
    
    
     DESCRIPTION OF THE PREFERRED EMBODIMENTS 
     Various other objects, features and attendant advantages of the present invention will be more fully appreciated as the same becomes better understood from the following detailed description of the preferred embodiments of the invention. 
     Preferably, the NM is in the form of a powder or crystals in the mixture. Likewise, the APM is preferably in the form of a powder or crystals in the mixture. Preferably, the mixture itself is in the form of a powder or crystals. Most preferably, the powder and/or crystals is a dry, free-flowing powder or crystals. 
     The crystalline form of powdery NM that is one of the active ingredients in the novel sweetener composition of the present invention is not particularly limited. For example, it may be either the known crystals (which may be also called “A-type crystal(s)”) or the “C-type” crystal(s) described below. The C-type is significantly superior to the former and is thus most preferred. 
     In an additional remark, the crystal structure of known NM as disclosed in W095/30689, the entire contents of which are hereby incorporated by reference, is described as IR spectrum data therein. Further, the present inventors analyzed the structure of its single crystal, and as a result, they confirmed that this crystal is a monohydrate, and when measured by powder X-ray diffractometry, the crystal shows characteristic peaks in diffractive X-ray (X-ray diffraction pattern) at diffraction angles of at least 6.0°, 24.8°, 8.2°, and 16.5° (2θ, CuKα radiation (ray;line)). For the sake of convenience, the present inventors referred to this crystal as “A-type crystal”. 
     The present inventors have also found that the water content of dry A-type crystal is usually in the range of 3 to 6% by weight (including crystal water), but if this A-type crystal is further dried until its water content is reduced to less than 3%, a novel crystal of N-(3,3-dimethylbutyl)-APM with improved dissolution rate wherein the crystal water has been eliminated, is obtained, and this novel crystal was referred to as “C-type crystal”. 
     Thus, a preferred embodiment of the present invention provides a novel form of N-{N-(3,3-dimethylbutyl)-L-α-aspartyl}-L-phenylalanine 1-methyl ester, which is referred to herein as the “C-type crystal”, methods of making, and methods of use. 
     When measured by powder X-ray diffractometry (using CuKαradiation), this C-type crystal shows characteristic peaks in the X-ray diffraction pattern at diffraction angles different from those of the A-type crystal, that is, at diffraction angles (20) of at least 7.1°, 19.8°, 17.3°, and 17.7°. Reference is made to Reference Examples 1 to 3 below. 
     APM that is the other active ingredient in the novel powdery sweetener composition of the present invention can be used in the form of e.g. the hydrated crystals, to which it is not particularly limited. Preferably, the APM is in the form of a dry, free-flowing powder. 
     The mixing ratio of NM and APM used in the novel sweetener composition of the present invention is in the range of 10 to 99.5% by weight in terms of the ratio of APM to 5 the total amount of both NM and APM. If the ratio of APM used therein is less than 10% by weight, the effect of APM on the promotion of NM dissolution is decreased, whereas with the ratio of more than 99.5% by weight, the effect of APM on the promotion of NM dissolution is hardly observed. More preferably, the mixing ratio ranges from 20 to 97%, more particularly preferably, the mixing ratio is 50 to 97%, more particularly preferably 55 to 10 95%, most preferably 60 to 90%, and most particularly preferably 75 to 85%. These ranges include all values and subranges therebetween, including 12%, 18%, 22%, 35%, 45%, 58% and 91%. 
     C-type crystals, when mixed with APM in the range of 10 to 99.5%, have significant promoting effect on dissolution rate and are thus superior to A type crystals, as can be seen from Tables 1 and 2 below. If C-type crystals are used as powdery NM, the ratio of APM to the total amount of NM and APM used therein is preferably in the range of 10 to 97% by weight, and more preferably 20 to 97%, more particularly preferably, the mixing ratio is 50 to 97%, more particularly preferably 55 to 95%, most preferably 60 to 90%, and most particularly preferably 75 to 85%. These ranges include all values and subranges therebetween, including 12%, 18%, 22%, 35%, 45%, 58% and 91%. 
     The dissolution rate of a mixture of NM C-type crystals and APM in the range of 10 to 90% by weight of APM thereto is higher than that of NM A-type crystals alone, and the dissolution rate of a mixture of NM C-type crystals and APM in the range of 50 to 97% by weight of APM thereto is higher than the dissolution rate of not only that of NM C-type crystals alone but also that of APM alone. Each of the above ranges includes all values and subranges therebetween, including 12%, 18%, 22%, 35%, 45%, 58% and 89%. 
     If NM and APM are separately and at the same time added to water (i.e., separate but simultaneous addition) without being previously mixed, preferably at the predetermined ratio, neither NM nor APM affect their mutual dissolution rate, and in this case, the dissolution rate as a whole is low but identical to the dissolution rate of one of them which has a lower dissolution rate when used alone. See, e.g., Experimental Example 3 below. 
     For the purpose of easy application or improvement in quality of sweetness, the novel sweetener composition of the present invention, similar to the case of conventional high-potency sweetener compositions, can incorporate diluents (thinners) and excipients such as sugar alcohols, oligosaccharide, food fibers (dietary fibers) and the like, or other synthetic high-potency sweeteners such as Alitame, saccharin, Acesulfame K etc. as necessary in an amount within such a range as not to spoil the NM and APM dissolution rate (solubilities) improved by the present invention. The diluents and excipients in this case include low-potency sweeteners such as sucrose, glucose or the like. 
     The sweetener composition according to the present invention is particularly suitable for use in food and drink compositions for human and animal consumption. Preferred examples include without limitations beverages, table-top sweeteners, sweetener packets, candies, ice cream, coffee, tea, cereal, liquid sweeteners, low-calorie sweeteners, gelatin desserts, bread, cookies, fruit flavored beverages, cake mixes, fruit juices, syrups, salad dressings, pet foods, carbonated and non-carbonated soft drinks, foodstuffs, and the like. The composition of the present invention is also suitable for other applications such as cough medicines, cough drops and tonics. The composition of the present invention may be suitably mixed with a diluent or solvent including aqueous-based, alcohol-based, mixed aqueous/alcohol-based, water, propylene glycol, a water/propylene glycol mixture, ethanol or a water/ethanol mixture. Preferably, the sweetener composition of the present invention may be used alone or will make up anywhere from 0.1% to greater than 99% by weight of the food or drink composition, more preferably 1-95%, more particularly preferably 2-90%, more especially preferably 5-85%, most preferably 10-75%, most particularly preferably 20-65%, and most especially preferably 30-55% by weight, based on the total weight of the food or drink composition. These ranges include all values and subranges therebetween, including 4%, 14%, 22%, 43%, 49%, 82% and 91%. 
     EXAMPLES 
     Having generally described this invention, a further understanding can be obtained by reference to certain specific examples which are provided herein for purposes of illustration only and are not intended to be limiting unless otherwise specified. The amounts are given as percentages by weight, except where otherwise mentioned. 
     Reference Example 1 
     Preparation of NM 
     The following were introduced successively under stirring to a reactor equipped with an agitating blade for ensuring very efficient transfer of gaseous hydrogen to a liquid layer. That is, 700 ml ion exchanged water, 4.21 ml of acetic acid, 20 g of 10% palladium carbon, 1,300 ml of methanol, 56 g of Aspartame and 25 ml of 3,3-dimethylbutylaldehyde were introduced. 
     The reactor was filled with a nitrogen gas stream, and then the reaction mixture was hydrogenated at a H 2  gas flow rate of 200 ml/min. at room temperature. The progress of this reaction was monitored by sampling the reaction mixture and analyzing the product in high performance liquid chromatography (HPLC). After the hydrogenation reaction for 6 hours, this reaction was terminated by filling the reactor with a nitrogen gas and filtering the reaction mixture through a fine pore filter (0.45 μm) to remove the catalyst. 
     As a result of the analysis of the resulting filtrate (1,494 g), the yield was 81%. Subsequently, this filtrate was concentrated to 281 g to remove the methanol, and crystals were precipitated under stirring at 10° C. overnight. Finally, 87 g white wet crystals of NM (yield: 77%) were obtained at a high purity (99% or more, HPLC) 
     Reference Example 2 
     Production of A-type crystals 
     A part of NM prepared in Reference Example 1 was used to prepare 100 g aqueous solution of NM at a concentration of 3% by weight (dissolved at 60° C.) . Then, the solution was cooled from 60° C. to 30° C. for 5 minutes under stirring. When the liquid temperature was reached to 30° C., crystallization of white crystals was initiated. After overnight aging under the liquid temperature kept at 30° C., the crystals were collected on a filter paper. 
     (a) The diffractive X-ray (X-ray diffraction pattern) of the wet crystals obtained above was measured by powder X-ray diffractometry (diffractometer) using CuKα ray(radiation). The obtained powder X-ray diffraction pattern is shown in FIG.  1 . 
     As is evident from the pattern of the figure, the wet crystals showed characteristic diffraction peaks at least 6.0°, 24.8°, 8.2°and 16.5°, and they were A-type crystals. 
     Further, (b) the wet crystals were placed in a vacuum dryer set at 50° C., and dried until their water content was reduced to 5% by weight. The dried crystals thus obtained were measured by powder X-ray diffractometer using CuKα Radiation (ray) , indicating that the crystals were A-type crystals as well. 
     Further, as a result of IR spectrum (KBr) measurement, its values agreed with those described in W095/30689, the entire contents of which being hereby incorporated by reference. 
     Reference Example 3 
     Production of C-type crystals 
     The dried A-type crystals with a water content of 5% by weight described above were continued to be dried in the vacuum dryer until their water content was reduced to 0.8% by weight. 
     The X-ray diffraction pattern of the dried crystals was measured by powder X-ray diffractometry (diffractometer) using CuKα ray. The thus obtained powder X-ray diffraction pattern is shown in FIG.  2 . 
     As is evident from the pattern of the figure, the dried crystals showed characteristic diffraction peaks at least at 7.1°, 19.8 17.3°, and 17.7°. As described above, the crystals are C-type crystals. 
     Experimental Example 1 
     (Dissolution rate of raw (original) powders each from NM C-type crystals and APM, and a mixture thereof) A predetermined amount of the sample was introduced into 900 ml water (20° C.) in a 1-L elution tester (the Japanese Pharmacopoeia, Paddle method, 100 rpm) and its dissolution time was measured (end point was visually confirmed). 
     Specifically, 1 g of sample taken from each mixture consisting of NM C-type crystal raw powder (average particle size of about 100 μm) and APM raw powder (average particle size of about 15 μm, IB-type bundled crystals) at the predetermined various ratios (APM content (% by weight)) shown in Table 1 below, was weighed, and then measured for its dissolution time. For comparison, 1.00 g, 0.90 g, 0.50 g, 0.10 g, 0.03 g, and 0.005 g samples were taken from said NM raw powder, and then their dissolution times were determined in the same manner as above. For the same purpose, 1.00 g, 0.97 g, 0.90 g, 0.50 g, and 0.10 g samples were taken from said APM raw powder, and then their dissolution times were determined in the same manner. 
     The dissolution time (min) (time needed for the dissolution) of each sample is shown in Table 1 below. 
     
       
         
               
             
               
               
             
               
               
               
             
               
               
               
               
             
               
             
               
               
               
               
             
           
               
                 TABLE 1 
               
               
                   
               
               
                 Dissolution times of NM C-type crystals, APM raw 
               
               
                 (original) powder, and mixture thereof 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 NM C-type crystals alone 
                   
               
               
                 (original powder) 
               
             
          
           
               
                 Weight of NM 
                 Dissolution 
                 APM alone (original powder) 
               
             
          
           
               
                 C-type crystals 
                 time 
                 Weight of APM 
                 Dissolution time 
               
               
                   
               
               
                   1.00 g 
                     62 min 
                   0.10 g 
                     10 min 
               
               
                 0.90 
                 60 
                 0.50 
                 20 
               
               
                 0.50 
                 55 
                 0.90 
                 27 
               
               
                 0.10 
                 40 
                 0.97 
                 29 
               
               
                 0.03 
                 30 
                 1.00 
                 30 
               
               
                  0.005 
                  4 
                 — 
                 — 
               
               
                   
               
             
          
           
               
                 Mixture (1 g) 
               
             
          
           
               
                 Content of APM 
                 Weight of 
                   
                   
               
               
                 original powder 
                 APM 
                 Weight of NM 
                 Dissolution time 
               
               
                   
               
               
                  10 weight % 
                   0.10 g 
                   0.90 g 
                     25 min 
               
               
                  50 
                 0.50 
                 0.50 
                 15 
               
               
                  90 
                 0.90 
                 0.10 
                 8 
               
               
                  97 
                 0.97 
                 0.03 
                 18 
               
               
                  99.5 
                  0.995 
                  0.005 
                 25 
               
               
                   
               
             
          
         
       
     
     As can be seen from this table, the dissolution rate (solubility) of the mixture thereof (the sweetener composition of the present invention) is always remarkably and significantly improved as compared with not only those of the NM C-type raw (original) crystals alone but also those of the APM alone. 
     The degrees of sweetness of NM and APM are respectively about 10,000 and about 200 times that of sucrose, as described above. From this viewpoint, the dissolution time of 1 g mixture should be compared with the dissolution time of an amount of NM necessary to achieve the same degree of sweetness, but even in such comparison, there is the promoting action of APM on the dissolution of NM, as follows. That is, the sweetness of 1 g mixture containing 50% APM raw (original) powder is equal to the sweetness of 0.51 g of NM alone, and the dissolution time of the former is 15 minutes, while the dissolution time of the latter is about 55 minutes, so there is a significant difference therebetween. 
     Experimental Example 2 
     (Dissolution rate of raw (original) powders each from NMA-type crystals and APM, and a mixture thereof) 
     The same experiment as that in Experimental Example 1 was conducted except that NM A-type crystal original powder (average particle size of 100 μm) was used in place of NM C-type crystal original powder. 
     The dissolution time (min) of each sample is shown in Table 2. 
     
       
         
               
             
               
               
             
               
               
               
             
               
               
               
               
             
               
             
               
               
               
               
             
           
               
                 TABLE 2 
               
               
                   
               
               
                 Dissolution times of NM A-type crystals, APM original 
               
               
                 powder, and mixture thereof. 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 NM A-type crystals alone 
                   
               
               
                 (original powder) 
               
             
          
           
               
                 Weight of NM 
                 Dissolution 
                 APM alone (original powder) 
               
             
          
           
               
                 A-type crystals 
                 time 
                 Weight of APM 
                 Dissolution time 
               
               
                   
               
               
                   1.00 g 
                     42 min 
                   0.10 g 
                     10 min 
               
               
                 0.90 
                 40 
                 0.50 
                 20 
               
               
                 0.50 
                 35 
                 0.90 
                 27 
               
               
                 0.10 
                 16 
                 0.97 
                 29 
               
               
                 0.03 
                 10 
                 1.00 
                 30 
               
               
                  0.005 
                 — 
                 — 
                 — 
               
               
                   
               
             
          
           
               
                 Mixture (1 g) 
               
             
          
           
               
                 Content of APM 
                 Weight of 
                   
                   
               
               
                 original powder 
                 APM 
                 Weight of NM 
                 Dissolution time 
               
               
                   
               
               
                  10 weight % 
                   0.10 g 
                   0.90 g 
                     35 min 
               
               
                  50 
                 0.50 
                 0.50 
                 25 
               
               
                  90 
                 0.90 
                 0.10 
                 23 
               
               
                  97 
                 0.97 
                 0.03 
                 29 
               
               
                  99.5 
                  0.995 
                  0.005 
                 30 
               
               
                   
               
             
          
         
       
     
     As can be seen from this table, the dissolution rate of the mixture (the sweetener composition of the present invention) is improved remarkably and significantly as compared with NM A-type crystals original powder alone. 
     It can also be seen that the dissolution rate of the mixture at a range of the certain mixing ratios (50 to 97% by weight of APM) is superior to that of APM powder alone (original powder). 
     In a similar comparison to that in Experimental Example 1, there is the promoting action of APM on the dissolution of NM, for example, as follows. That is, the sweetness of 1 g mixture containing 50% APM original powder is equal to the sweetness of 0.51 g of NM alone, and the dissolution time of the former is 25 minutes, while the dissolution time of the latter is about 35 minutes, so there is a significant difference therebetween. 
     Experimental Example 3 
     (Separate addition of NM original powder and APM original powder) 
     The same NM and APM as in Experimental Example 1 were used, and the dissolution time was determined in the same manner as that in Experimental Example 1. 
     That is, 0.5 g each of both of them was weighed (1.0 g in total) and introduced simultaneously without being previously mixed, into the elution tester (separate addition). The results are shown in Table 3 below. For reference, the dissolution time of 0.5 g NM original powder alone (Experimental Example 1) is also shown together in the table. 
     
       
         
               
             
               
               
               
             
           
               
                 TABLE 3 
               
             
             
               
                   
               
               
                 Dissolution time of NM and APM when separately added 
               
             
          
           
               
                   
                   
                 Separate addition (1 g in total) 
               
               
                   
                 NM C-type crystals alone 
                 (0.5 g NM C-type crystals/0.5 g 
               
               
                   
                 (0.5 g) 
                 APM original powder) 
               
               
                   
                   
               
               
                   
                 55 minutes 
                 55 minutes 
               
               
                   
                   
               
             
          
         
       
     
     From this table, the improvement of NM dissolution rate (solubility) by APM is not observed when NM and APM are separately added without being previously mixed. 
     According to the present invention, Aspartame (APM) is mixed with Neotame (NM) whereby poor dissolution characteristics of NM can be significantly improved, and further, the dissolution rate of APM can also be improved depending on the mixing ratio. 
     According to the present invention, Aspartame (APM) is mixed with Neotame (NM) whereby the poor dissolution characteristics (solubility) of NM can be significantly improved, and simultaneously a sweetener excellent in quality of sweetness can be easily obtained. Accordingly, the present invention is advantageous particularly for use in drinks where a sweetener is dissolved in industrial production, but the present invention is not limited thereto and can be used as an improved sweetener composition in any uses. 
     Having now fully described this invention, it will be apparent to one of ordinary skill in the art that many changes and modifications can be made thereto without departing from the spirit or scope of the invention as set forth herein. 
     This application is based on International Application No. PCT/JP99/02198, filed Apr. 26, 1999, and Japanese Patent Application No. 10-125989, filed May 8, 1998, the entire contents of each of which being hereby incorporated by reference, the same as if set forth at length.