Abstract:
The present invention describes a new class of antigen binding molecules which contain Fv-fragments of an antibody but do not use the constant antibody domains. They can also dimerize with other antibody fragment molecules or with non-antibody fragment molecules to form bi- or multifunctional antibody-fragment fusion proteins and so-called miniantibodies, respectively. The new fusion proteins can be used in the broad field of diagnostic and therapeutical medicine.

Description:
The present invention describes a new class of antigen binding molecules which contain Fv-fragments of an antibody but do not use the constant antibody domains. They can also dimerize with other antibody fragment molecules or with ace non-antibody fragment molecules to form bi- or multifunctional antibody-fragment fusion proteins and so-called mininantibodies, respectively. The new fusion proteins can be used in the broad field of diagnostic and therapeutical medicine. 
     BACKGROUND OF THE INVENTION 
     Since a few years there is a great interest in the biotechnological field to modify naturally occurring antibodies in order to obtain more specified and more individual antibody species. Therefore, attempts have been made to produce (modified) antibody fragments. 
     All naturally occurring antibodies of all classes have at least two binding sites. This enables them to bind to a surface with a greater functional affinity (also called avidity) than monovalent fragments, such as Fab fragments. Over the last few years, methods have been described (Skerra and Pluckthun, 1988, Science 240, 1038-1040; Better et al., 1988, Science 240, 1041-1043) with which functional antibody fragments can be produced in Escherichia coli. These include the Fv fragment (the heterodimer consisting of V H  and V L ) and the Fab fragment (consisting of the complete light chain with the domains V L  and C L  as well as the first two domains of the heavy chains V H  and C H1 ). 
     The Fv fragment, however, has a tendency to dissociate into V H  and V L  and therefore, it is advantageous to link the two domains covalently. One particular way of linking them is by designing a peptide linker between them, either in the orientation V H  -linker-V L  or V L  -linker-V H  (Bird et al.,1988, Science 242, 423; Huston et al., 1988, Proc. Natl. Acad. Sci. USA 85, 5879) The resulting fragments are called single-chain Fv fragments. 
     All these fragments are, however, monovalent. We describe in this invention a method to engineer small dimerization domains based on peptides forming amphipathic helices. These peptides will be referred to as &#34;intercalating&#34;, but this term is only meant to express the ability of targeted association and not a restriction referring to a particular structure of the dimerization interface. 
     While the methodology described here, is in principle applicable to either Fab, Fv or scFv fragments, it is the latter for which their use is most advantageous. In this case bivalent fragments can be constructed of very small size, and still the dissociation into V L  and V H  as well as the wrong matching of fragment chains, e.g. V L  --V L , can be prevented. 
     Antibody fragments of small size are of particular advantage in many applications. In diagnostic applications (e.g. ELISA, RIA, etc.), the smaller molecules surface decreases the problems of nonspecific interactions, which are known to frequently involve the constant domains. The same is true in using antibody fragments as ligands in affinity chromatography. In tumor diagnostics or therapy, it is important that a significant proportion of the injected antibody penetrates tissues and localizes to the tumor, and is dependent on the molecular dimensions (Colcher et al., 1990, J. Natl. Cancer Inst. 82, 1191-1197). Expression yields and secretion efficiency of recombinant proteins are also a function of chain size (Skerra &amp; Pluckthun, 1991, Protein Eng. 4, 971) and smaller proteins are preferred for this reason. Therefore, molecules of a small size are advantageous for several reasons. 
     Previously, decreasing the molecular dimensions of the antibody meant the preparation of proteolytic fragments. The smallest bivalent fragments, (Fab)&#39; 2  fragments, are still about twice the size of the present fragments of this invention. Therefore, these new fragments combine three features: (a) small size, (b) bivalence or bifunctionality and (c) ability of functional expression in E. coli. 
     There is great interest in bifunctional antibodies in a large number of areas. Bifunctional antibodies may be defined as having two different specificities for either two different antigens or for two epitopes of the same antigen. 
     There are currently a number of methods how to produce bifunctional antibodies. However, none of the existing methods allows to produce exclusively bifunctional antibodies in vivo, but rather a mixture of molecular species always occur, requiring complicated and expensive separation procedures. 
     Four principal methods can be distinguished. In the first, chemical crosslinking is used, which may advantageously use heterobifunctional crosslinkers. By this method, whole antibodies (Staerz et al., 1985, Nature 314, 628; Perez et al, 1985, Nature 316, 354-356), Fab fragments (Carter et al., 1992, Biotechnology 10, 163) and scFv fragments (Cumber et al., 1992, J. Immunol. 149, 120) have been chemically crosslinked after purification. 
     The second previous method involved the fusion of two hybridomas to give a so-called heterohybridoma or &#34;quadroma&#34;. In this method, any light chain can pair with any heavy chain, and the two heavy chains can give homodimers or heterodimers resulting in very complicated product mixtures (Milstein &amp; Cuello, 1983, Nature 305, 537). 
     The third method is related to the second and consists of transfecting two expression plasmids into a hybridoma cell, encoding the heavy and light chain of the second antibody (Lenz &amp; Weidle, 1990, Gene 87, 213) or a retroviral vector (De Monte et al., 1990, Acad. Sci. 87, 2941-2945). However, once introduced, the product mixture is identical as in the second procedure. 
     Finally, antibodies have been reduced, mixed and reoxidized (Staerz &amp; Bevan, 1986, Immunology Today 7). Again, very complicated product mixtures are obtained requiring sophistical separation and quality control procedures. 
     Thus a method is still needed allowing the isolation of exclusively heterodimeric antibodies directly without the complicated preparation required from chemical crosslinking. In the present invention, this problem is solved by (i) covalently linking corresponding VH and VL domain in a scFv fragment and (ii) using dimerization domains only allowing the formation of heterodimers, such as certain leucine zippers and derivatives. 
     Another important consideration in the present invention was the desire to make the MW of the bispecific antibody as small as possible for reasons explained above in detail. This was achieved by using scFv fragments. 
     A number of uses of bispecific antibodies bave been described, and most of them would benefit from this new technology. For example, bispecific antibodies are of great interest in tumor therapy. One arm of the antibody may bind to a tumor marker, the other arm to a T-cell epitope, a toxin, or a radionuclide binding peptide or protein to bring a killing function close to the tumor cell. In diagnostics, one arm may bind to the analyte of interest and the other to a principle which can easily be quantified, e.g. an enzyme. Finally, in cellular applications, it may be advantageous to obtain higher selectivity in binding, if two different epitopes or the same protein complex can be recognized or if two different proteins can be recognized on the same cell surface. 
     Thus, it was object of the invention to create new individual and stable antibody fragment fusion proteins with bi- or even mulitfunctional binding sites. 
     It has been found that antibody fragment fusion proteins containing Fv-fragments could be produced by genetic engineering methods which show specified and improved properties. 
     Object of the invention is, therefore, a monomeric antibody-fragment fusion protein essentially consisting of a Fv-fragment of an antibody and a peptide which is capable to dimerize with another peptide by noncovalent interaction. 
     The term &#34;noncovalent interaction&#34; means every existing under normal condititions stable linkage which is not related to a covalent binding, for example linkage by Van der Waal&#39;s forces, (steric) interdigitation of amphiphilic peptides, especially peptide helices, or peptides bearing opposite charges of amino acid residues. The correspondingly effective peptides are called above and below interactive or intercalating peptides. 
     The amphiphilic peptides consist of up to 50 amino acids. Preferrably they consist of 10 to 30 amino acids. In a preferred embodiment of the invention the interactive peptide is a peptide helix bundle (comprising of a helix, a turn and another helix, see above). In another embodiment the interactive peptide is a leucine zipper consisting of a peptide having several repeating amino acids, in which every seventh amino acid is a leucine residue. In other cases according to the invention the peptide bear positively or negatively charged residues, e.g. lysine (positively charged) or glutamic acid (negatively charged) in a way that this peptide can bind to another peptide (of a second monomeric unit) bearing opposite charges. 
     The Fv-fragment and the intercalating peptide are linked together either directly or by a linker peptide, preferrably by a linker peptide. In a preferred embodiment the linker peptide is a hinge region sequence of an antibody. 
     As defined, the Fv-fragment consists of the V L  and V H  region of an antibody. The Fv-fragment according to the invention is preferrably a single chain fragment. Single chain fragments can be obtained by standard techniques using standard linker molecules. 
     Furthermore, object of the invention is a dimeric fusion protein essentially consisting of two monomeric fusion proteins, wherein the linkage of the monomeric units bases on noncovalent interaction of identical or different peptides, characterized in that at least one monomeric unit is an antibody-Fv-fragment fusion protein as defined above. 
     If the dimer contains two Fv-fragments, the Fv-fragments may be the same (identical antigen binding sites) or may be different (different antigen binding sites). In these cases mono- and bispecific (Fv)-miniantibodies can be obtained. According to the invention bispecific mini-antibodies are preferred. 
     The interactive peptides may be the same or may be different; preferrably, they are identical. The intercalating peptides may be associated in parallel or in antiparallel fashion. 
     Object of the invention is, therefore, above all, a dimeric fusion protein consisting of two Fv-fragments with different specificity (antigen binding sites) and identical intercalating helix peptides, the antibody fragments and the peptides are linked together by a hinge region sequence. 
     Furthermore, object of the invention is a dimer consisting of a monomeric unit containing a Fv-fragment and another monomeric unit wherein the Fv-Fragment was replaced by a non-antibody peptide. The non-antibody peptide may be a toxin, like ricin, a chelator- or metal binding peptide, or an enzyme (e.g. marker enzyme), or a peptide bearing a detectable label (e.g. a radioisotope). 
     The non-antibody peptide can also bear a corresponding binding site for said groups, binding sites directed to T-cells or T-cell fragments included. 
     Furthermore, the invention relates to monomers and diners, as defined above, wherein the interactive peptide(s) is (are) additionally fused at the C-terminus to target proteins/peptides as mentioned above, the corresponding binding sites included. Thus, the resulting fusion proteins and miniantibodies, respectively, are multifunctional. 
     The invention relates, furthermore, to a process for preparation of a monomeric antibody fusion protein as defined above, characterized in that the genes coding for the Fv-fragment, the interactive peptide and, if desired, the linking peptide are cloned into one expression plasmid, a host cell is transformed with said expression plasmid and cultivated in a nutrient solution, and the monomeric fusion protein is expressed in the cell or secreted into the medium. 
     Object of the invention is, finally, a process for preparation of a dimeric fusion protein as defined above, characterized in that the genes coding for the complete monomeric fusion proteins or parts of it are cloned at least into one expression plasmid, a host cell is transformed with said expression plasmid(s) and cultivated in a nutrient solution, and either the complete dimeric fusion protein is expressed in the cell or into the medium, or the monomeric fusion proteins are separately expressed and the noncovalent linkage between the two monomeric units is performed in the medium or in vitro, and in the case that only parts of the fusion proteins were cloned, protein engineering steps are additionally performed according to standard techniques. 
     The dimeric Fv-fragments containing fusion proteins according to the invention show a high avidity against corresponding antigens and a satisfying stability. These novel bivalent or bifunctional molecules can be prepared as folded and assembled molecules in E. coli. These miniantibodies are compatible with functional expression by secretion. 
     DETAILED DESCRIPTION OF THE INVENTION 
     The oligomerization domains were selected for having a fairly small molecular weight and for being compatible with transport of the fusion protein through the membrane. They are based on two different types of amphiphilic helices. 
     Amphiphilic helices are known to predominantly, but not exclusively, associate in two different molecular structures: Four helix bundles and coiled coils. The design and formation of helix bundles has been studied previously (Eisenberg et al., 1986, Proteins 1, 16-22; Ho and deGrado, 1987, J. Am. Chem. Soc. 109, 6751-6758; Regan and deGrado, 1988, Science 241, 976-978; Hill et al., 1990, Science 294, 543-546). This molecule association is also known from natural proteins (Richardson, 1981, Adv. Prot. Chem. 34, 167). 
     The four helix bundle may be formed from either four separate molecules (each contributing one helix), two molecules containing two helices each (connected as helix-turn-helix) or one molecule containing a helix-turn-helix-turn-helix-turn-helix motif. For dimerization or multimerization, only the first two are suitable. 
     Three variations of this latter theme were tested. In the first, one helix of the sequence given in Eisenberg et al. (1986) (Proteins 1, 16-22) was used. In the second, this sequence was extended by a small hydrophilic peptide ending in a cysteine. Once the helices are associated, the hydrophilic peptides are held in sufficiently close contact that they can collide and a disulfide bond can form under oxidizing conditions, as in the periplasm of E. coli. In the third variation, two helices are used in tandem, separated by a short turn encoding peptide. 
     In the second design, peptides are used which can form so-called coiled-coil structures. Such peptides occur in transcription factors such as e.g. GCN4 from yeast and have been called leucine zippers (Landschulz et al., 1988, Science 240, 1759-1764). The crystal structure of this has been solved recently (O&#39;Shea et al., 1991, Science 254, 539-544) and showed a parallel arrangement of the helices. 
     A covalent attachment of the helices is possible by a small peptide extension, again containing a cystein. Since the helices are now parallel, the peptide extension can be much shorter, since the distance is much smaller. 
     The various dimerization devices (intercalating helices) were however not fused to the antibody domain directly. It is advantageous to introduce a flexible peptide between the end of the scFv fragment and the beginning of the helix. As an example, the upper hinge region of mouse IgG3 has been used. However, a variety of hinges can be used. It is not required for dimerization per se, but provides a spacing of the two scFv domains similar to the antigen binding sites of a whole antibody. This way, the two binding sites span a greater distance in space and therefore can reach neighboring antigens on a solid surface. 
     The naturally occurring hinges of antibodies are preferred embodiments of hinges in bivalent miniantibodies. In the case of bifunctional miniantibodies, the hinges may be shorter, since frequently molecules from different surfaces are to be crosslinked as close as possible, and flexibility of the dimer is not necessary. The choice of the hinge is governed by the desired residue sequence, length (Argos, 1990, J. Mol. Biol. 211, 943-958), compatibility with folding and stability of the amphiphilic helices (Richardson &amp; Richardson, 1988, Science 240, 1648-1652), secretion and resistance against proteases. 
     The present invention deals with peptides as dimerization devices, which should be as small as possible. One preferred embodiment is the use of peptides which can form amphipathic helices. Such helices shield the hydrophobic surface by dimerization or even multimerization. Helices of this type are characterized by their having hydrophobic patches on one face of the helix, and containing a sufficient number of helix-forming residues. Rules for such peptides are discussed in Eisenberg et al. 1986, O&#39;Shea et al.,.1991 (Science 254, 539-544),1992 (Cell 68, 699-708). 
     Natural peptides of this type are found as the so-called leucine zippers, characterized by a periodic occurence of leucine (every seventh residue) and other hydrophilic residues (e.g. valine) also every seventh residue. As these principles are now understood (O&#39;Shea et al. 1991, 1992, literature cited), the sequence can be varied to incorporate residues which make the association of homodimers unfavorable, but favors the association of heterodimers. Such sequence alteration can e.g. involve the incorporation of charge bridges, such that in the homodimers, like charges repel each other and in the heterodimer, opposite charges attract each other (see below). 
     The present invention can also be extended to bifunctional miniantibodies. In this case, dimerization devices (intercalation peptides) have to be used which will only allow the formation of heterodimers, but not homodimers. A preferred embodiment of this part of the invention are two different coiled-coil helices, such as in naturally occurring leucine zippers, e.g. from the transcription factor proteins jun and fos (O&#39;Shea et al., 1989, Science 245, 646-648). 
     In a further embodiment of the invention, the constant scFv-hinge-helix can be extended at the C-terminus to result in a fusion protein. For example, a fusion to an enzyme may be made to use such bivalent constructs in diagnostics. Such enzymes are e.g. alkaline phosphatase, luciferase or horse radish peroxidase. The advantage of such a antibody-enzyme fusion protein would be that the bivalence of the antibody would lead to an enhanced binding to the surface-bound antigen. The advantage over a fusion protein prepared by conventional technology (i.e. chemical coupling of the antibody to the enzyme of choice) would be a greater batch-to-batch consistency; homogeneity of the product and the much simpler method of preparation, namely from E. coli in a single step. 
     In the same fashion, the miniantibodies may be extended at the C-terminus to incorporate a toxin. Such immunotoxins would be bivalent or even bispecific and thus combine the advantages of such antibody fragments linked above with the advantages in tumor therapy known for immunotoxins. Similarly, a metal binding peptide or protein could be linked genetically to be used in radioimmunotherapy or in tumor imaging. The same advantages for any genetically encoded hybrid protein hold true as given above for the antibody-enzyme fusions. 
     In another embodiment of the invention, a construct of the type scFv-hinge-helix may be made to dimerize with another protein fused to a dimerization domain, in complete analogy as described above for the formation of bispecific miniantibodies. In this fashion, the scFv fragment would e.g. be fitted with the helix of the fos protein. Such foreign protein, which could be made to form heterodimers with the scFv fragment, include enzymes useful in diagnosis, toxins, metal-binding peptides or proteins useful in radioimmunotherapy or radio-imaging. 
     Using the principles of this invention, the dimerization domains presented here can also serve for purification purposes. A recombinant protein of any kind can be fused to a dimerization domain, e.g. to hinge-fos-zipper. After coexpression with a scFv-hinge-jun, the heterodimer can be purified in one step with an affinity column for the scFv-specificity. In an alternative approach, the `opposite` zipper, linked to a column support, `catches` the protein-hinge-zipper when passing through the column as a crude cell extract. 
     The elution of the pure fusion protein from the column is possible using the unfolding temperature of the zipper. A subsequent separation from the dimerization domain is achievable by introduction of a proteolytic site, e.g. for blood clotting factor Xa, into the hinge (Nagai &amp; Thogerson, 1987, Meth. Enyzmol. 152,461-481). 
     A particular advantage of the miniantibodies described in this invention is the ability to assemble functionally in Escherichia coli. In the case of homobivalent constructs, a dimerization principle is used which allows the formation of homodimers. Examples described above include the coiled-coil helix (leucine zipper) of the yeast protein GCN4 or the helices from an antiparallel 4-helix bundle. In this case, the scFv fragment is expressed in the presence of a bacterial signal sequence and carries at the end of the gene of the scFv fragment the codons for a hinge and the dimerization helix or helix-turn-helix. The helices are compatible with secretion to the periplasmic space in E. coli, where protein folding, disulfide formation and assembly occurs. Under these conditions, the homodimeric proteins form by themselves and can directly be isolated in the dimeric form. 
     If heterobivalent constructs are desired, two different scFv fragments or one scFv fragment associating with a different protein need to associate. In the preferred embodiment of this invention, both proteins to be assembled are expressed in the same cell, preferably on the same plasmid, preferably as a dicistronic operon. The design of artificial dicistronic operons is explained e.g. in Skerra et al. (1991, Protein Eng. 4, 971). Since the assembly must take place in the periplasm, because the scFv fragment can only fold in the oxidizing milieu, both proteins must be transported and both must be fitted with a signal sequence. The dimerization peptides must be chosen such that they promote the association of two different proteins, but prevent the association of the respective homodimers. Examples of such proteins are the leucine zipper peptides of the proteins fos and jun (see above). 
     When not expressed in the same cell, the different scFv-hinge-zipper constructs have to be mixed together as a crude cell extract or purified protein and treated with raised temperature. In absence of the `opposite` zipper, e.g. a scFv-hinge-jun-zipper construct is able to form homodimers. After short heating to the melting temperature of around 40˜C, the zippers of the unwanted homodimer unfold and form a much more stable heterodimer (O&#39;Shea et al., 1992, Cell 68, 699-708). Without raising the temperature, formation of heterodimers in vitro is not possible, as tested in experiments. 
    
    
     BRIEF DESCRIPTION OF THE FIGURES AND THE SEQUENCE LISTING 
     FIG. 1 scFv-Expression vector pLISC-SE containing the scFv-fragment. 
     FIG. 2 Dicistronic scFv-hinge-zipper expression vector pACKxFyJ. 
     FIGS. 3a and 3b Functional ELISA; The concentrations of the affinity purified proteins, measured by OD 280  (vertical axis), refer to the molar number of binding sites per well (horizontal axis). The ELISA plates were coated with phosphocholine-BSA, and the purified phosphocholine-specific miniantibody-proteins were bound and detected by an anti-McPC603 antiserum. (3a) Comparison of various miniantibodies. (3b) Comparison of miniantibody scHLXc with ScFV and whole IgA. 
     FIG. 4 Functional Anti-lysozyme ELISA; PC-affinity purified samples of coexpressed anti-PC-anti-lysozyme bispecific miniantibody. + and - on the horizontal axis means: plus inhibitor (+) and without inhibitor (-). 
    
    
     The attached sequence listing refers to sequence identity numbers (SEQ ID NO:): 
     SEQ ID NO:1-2 Whole nucleotid- and amino acid sequence of the pLISC-SE vector. 
     SEQ ID NO:3-4 Gene cassette of intercalating GCN4-leucine zipper (nucleotid- and amino acid sequence). 
     SEQ ID NO:5-6 Gene cassette encoding intercalating antiparallel helix-turn-helix (nucleotid- and amino acid sequence). 
     SEQ ID NO:7-8 Gene cassette encoding intercalating jun-zipper and IgG3-hinge region. 
     SEQ ID NO:9-10 Gene cassette encoding intercalating fos-zipper and IgG3-hinge region. 
     SEQ ID NO:11-12 Gene cassette encoding intercalating jun-zipper and designed linker. 
     SEQ ID NO:13-14 Gene cassette encoding intercalating fos-zipper and designed linker. 
     EXAMPLE 1 
     Construction of Vectors for Secreted Single-chain Fragments, Containing a Restriction Site for Introducing Genes for Intercalating Peptides. 
     Recombinant DNA-techniques were based on Sambrook et al. (1989, Molecular Cloning: A laboratory manual. Second edition. Cold Spring Harbor Laboratory, New York). Functional expression of the single-chain Fv fragments and the miniantibodies in E. coli JM83 was carried out with vectors similar to pASK-lisc (Skerra et al., 1991, Protein Eng. 4, 971). Site directed mutagenesis was directly performed in these vectors according to Kunkel et al. (1987, Meth. Enzymol. 154, 367-382) and Geisselsoder et al. (1987, Biotechniques 5, 786-791) using the helper phage M13K07 (Vieira &amp; Messing, 1987, Meth. Enzymol. 153, 3-11). SDS-PAGE was carried out as described by Fling and Gregerson (1986, Anal. Biochem. 155, 83-88). Concentrations of affinity-purified proteins were measured by OD 280  using calculated extinction coefficients (Gill &amp; von Hippel, 1989, Anal. Biochem. 182, 319-326).A vector such as pASK40 (Skerra et al., 1991, Protein Eng. 4, 971) is used, which contains an origin of replication, a regulatable promotor, a bacterial signal sequence followed by a multiple cloning site, a transcription terminator and an origin for single stranded phages. The gene for the single-chain Fv fragment is designed as follows: The nucleotide sequence of a V H  domain is directly followed by a linker sequence encoding preferably about 15 residues, preferably of the sequence (Gly 4  Ser) 3 , followed directly by the sequence of the V L  domain. Alternatively, the sequence of the V L  domain may be directly followed by the sequence of the linker, followed by the sequence of the V H  domain. 
     If the antibody is of known sequence, the complete gene of the scFv fragment may be assembled from synthetic oligonucleotides. A detailed experimental procedure for such a gene synthesis of an antibody gene is e.g. given in Pluckthun et al. (1987, Cold Spring Harbor Symp. Quant. Biol. 52, 105-112). 
     If the genes of the V H  and V L  domains are present in other vectors, the gene for the scFv fragment may be assembled from restriction fragments. For example, a restriction fragment encoding most of the V H  domain may be excised from another plasmid, and a fragment encoding most of the V L  domain may be excised from a plasmid. The remaining pieces of V L  and V H  and the linker for the scFv fragment can be provided by cassettes of synthetic oligonucleotides, which need to be ligated by standard methodology (Sambrook et al., 1989, literature cited). The mixture of fragments is ligated into the vector pASK40 or a similar plasmid containing a pair of suitable restriction sites. 
     If the genes of the antibody have not been cloned before, they may be directly obtained from the hybridoma cell producing the antibody by the polymerase chain reaction (PCR; PCR methodology is described in McPherson et al., 1991, PCR-A Practical Approach Oxford University Press, New York). Primers suitable for amplification of V H  and V L  domains have been given by Orlandi et al., 1989, Proc. Natl. Acad. Sci. USA 86, 3833-3837; Huse et al., 1989, Science 246, 1275-1281; Larrick et al., 1989, Bio-technology 7, 934-938. The methodology of obtaining mRNA from hybridoma is described in these references as well. The separate V H  and V L  genes may be cloned into separate vectors, and the scFv gene assembled according to the principles explained above. 
     If the ligated fragments do not result in a correct reading frame of the scFv fragment, a precise fusion with the signal sequence codons resident on the plasmid may be generated by site directed mutagenesis. The design of the oligonucleotides and the execution is possible for anyone skilled in the art. 
     The scFv expression plasmid so obtained contains the codons for a bacterial signal sequence, directly followed by the first variable domain (V H  or V L ), a linker and the second variable domain (V L  or V H ) under the control of a regulatable promotor. 
     At the 3&#39; end of this genes, corresponding to the C-terminus of the scFv protein, a unique restriction site is introduced into the expression plasmid to allow insertion of cassettes coding for the intercalating peptides. The restriction site is introduced by site directed mutagenesis using the method of Kunkel (1987, Meth. Enzymol. 154, 367-382). 
     An example of the complete sequence of a suitable single-chain Fv expression plasmid pLISC-SE for receiving an intercalation peptide is shown in FIG. 1 and Sequence Identity No. SEQ ID NO:1. 
     EXAMPLE 2 
     Design and Construction of a Gene Cassette Encoding Intercalating Peptides of a Leucine Zipper. 
     The gene cassette, fitted with restriction sites to be compatible with the restriction site at the 3&#39; end of the scfv fragment gene, must encode the sequence of a hinge (connection the scFv fragment to the intercalating peptide) and the intercalation peptide itself. The hinge region, may however also be obmitted. 
     As an example the sequence of the upper hinge region of mouse IgG3 (Dangl et al., 1988, EMBO J. 7, 1989-1994), followed by the sequence of the leucine zipper sequence of the yeast protein GCN4 (Oas et al., 1990, Biochemistry T29, 2891-2894), is back-translated into frequently used E. coli codons SEQ ID NO:3 oligonucleotides are synthesized, and ligated into the vector pLISC-SE, previously digested with EcoRI and Hind III. 
     EXAMPLE 3 
     Design and Construction of a Gene Cassette Encoding Intercalating Peptides of a Four-helix Bundle. 
     Analogous to Example 2, the sequence of the upper hinge region of mouse IgG3, followed by the sequence of the helix-turn-helix of a four helix bundle (Eisenberg et al., 1986, literature cited) is backtranslated into frequently used E. coli codons (SEQ ID NO:5). Oligonucleotides are synthesized, and ligated into the vector pLISC-SE, previously digested with EcoRI and Hind III. 
     EXAMPLE 4 
     Design and Construction Two Gene Cassettes Encoding Intercalating Peptides of a Leucine Zipper and their Co-expression. 
     Analogous to Example 2, the sequence of the upper hinge region of mouse IgG3 followed by the sequence of the zipper sequence of the jun protein (O&#39;Shea et al., 1992, literature cited), is backtranslated into frequently used E. coli codons (SEQ ID NO:7). Oligonucleotides are synthesized, and ligated into the vector pLISC-SE, previously digested with EcoRI and Hind III. 
     In a parallel reaction, the sequence of the upper hinge region of mouse IgG3, followed by the sequence of the zipper sequence of the fos protein (O&#39;Shea et al., 1992, Cell 68, 699-708), is backtranslated into frequently used E. coli codons SEQ ID NO:9. Oligonucleotides are synthesized, and ligated into the vector pLISC-SE, previously digested with EcoRI and Hind III. The two vectors thus each code for a different antibody scFv fragment, followed by a hinge peptide and a different leucine zipper peptide. To co-express the two scFv fragments, the whole scFv-hinge-zipper gene of the fos-containing product is excised from the vector as a Xba I-Hind III fragment and ligated into the vector, pLISC-SE-scFv-jun, containing already the scFv gene of the other antibody. 
     The newly obtained vector then expresses the scFv 1  -linker 1  -fos-zipper and the scFv 2  -linker 2  -jun-zipper from a single promoter as a dicistronic operon. 
     An improved sequence for the hinge region in the context of jun and fos SEQ ID NO:12 and 14. This hinge is shorter and therefore not as susceptible to proteolysis. In cases, where the distance between the two binding sites is of less importance, such shortened hinges may be advantageous. In this case, the &#34;tail&#34; of the scFv fragment has been shortened and the EcoRI site, which receive the genes for the intercalating peptides has been moved four residues upstream. 
     EXAMPLE 5 
     Purification of Bivalent Miniantibody from E. coli. 
     E. coli JM83, harboring a plasmid constructed as in examples II and III, are grown to an O.D. 550 of 0.5 and induced with IPTG at a final concentration of 1 mM. The cells are centrifuged, resuspended in BBS buffer (200 mM Na-borate, 160 mM NaCl, pH 8.0) and the suspension is passed through a French press. In these examples, a phosphorylcholine binding miniantibody is used. The miniantibody is purified by passage over a phosphorylcholine affinity chromatography as described (Chesebro and Metzger, 1972, Biochemistry 11, 766-771) 
     EXAMPLE 6 
     Purification of a Bispecific Miniantibody from E. coli 
     E. coli JM83, harboring a plasmid constructed as in examples II and III and containing a dicistronic structural gene for two different scFv (FIG. 2), are grown to an O.D. 550 of 0.5 and induced with IPTG at a final concentration of 1 mM. The cells are centrifuged, resuspended in BBS buffer (200 mM Na-borate, 160 mM NaCl, pH 8.0) and the suspension is passed through a French press. 
     In this example, a bispecific miniantibody is used containing both a specificity for phosphorylcholine as well as benzoylampicillin. The miniantibody is purified by passage over a phosphorylcholine affinity chromatography as described (Chesebro and Metzger, 1972, literature cited) 
     EXAMPLE 7 
     Surface Binding of Bivalent Miniantibodies 
     The ELISA-plates (Nunc, Macrosorp) were coated with 400 ˜g/ml phosphocholine-BSA in PBS buffer (20 mM phosphate, pH 7.2, 115 mM NaCl). The hapten reagent was prepared from nitrophenyl phosphocholine (Sigma), which was reduced and diazotized essentially as described (Chesebro &amp; Metzger, 1972, literature cited), and reacted by azo-coupling to BSA (Sigma) in borate-saline buffer (52.5 mM sodium borate, pH 9, 120 mM NaCl) at 4°˜C. for 48 hours with subsequent dialysis against PBS. After blocking the non-coated plate surface with 5% skim milk (Nestle) in PBS buffer for at least 2 hours, the periplasmic extract or the purified protein was incubated in BBS buffer on the plate for 90 min at room temperature. After thorough washing (3 times), remaining functional antibody fragments were detected according standard procedures (Harlow &amp; Lane, 1988, &#34;Antibodies, A Laboratory Manual&#34;, Cold Spring Harbor Laboratory, 555-592) with rabbit anti-McPC603 serum and anti-rabbit immunoglobulin linked to peroxidase (Sigma) according to Gallati (1979, Clin. Chem. Clin. Biochem. 17, 1-4). 
     An enormous gain in binding, and thus sensitivity, is observed for all miniantibody constructs, compared to the monomeric scFv fragment. This is consistent with the simultaneous binding of two or even more binding sites to the same surface. These avidity of the fusion protein scHLXc was comparable to the natural antibody McPC603, which could be detected with antigen-coated ELISA, while the monomeric scFv fragment could only be detected with hundred-fold higher concentrations (FIGS. 3a, b). All binding is nearly totally inhibitible with soluble hapten, except of the monomeric scFv fragment. The thermodynamic affinity of the natural antibody to soluble phosphocholine is about 1.6 $ 10 5  M -1  and thus relatively weak (Metzger et al., 1971, Proceedings of the I St  Congress of Imunology. Academic Press, New York, pp. 253-267), and this is apparently not sufficient for a monomeric fragment-hapten complex to survive the repeated washing steps of a functional ELISA (Kemeny &amp; Challacombe, 1988, &#34;ELISA and other solid phase immunoassays&#34;, Wiley &amp; Sons, New York). 
     EXAMPLE 8 
     Surface Binding of Bifunctional Miniantibodies 
     Coexpressed functional miniantibodies recognizing phosphorylcholine with one arm and lysozyme with the other arm were purified by phosphocholine (PC) affinity chromatography and tested for lysozyme specificity. An ELISA-plate was coated with lysozyme, the ELISA was carried out as described in Example VII. Three different preparations show binding to the antigen-surface, which is completely inhibitible with soluble lysozyme (FIG. 4). 
     
         __________________________________________________________________________#             SEQUENCE LISTING- (1) GENERAL INFORMATION:-    (iii) NUMBER OF SEQUENCES: 14- (2) INFORMATION FOR SEQ ID NO: 1:-      (i) SEQUENCE CHARACTERISTICS:#pairs    (A) LENGTH: 4515 base     (B) TYPE: nucleic acid     (C) STRANDEDNESS: double     (D) TOPOLOGY: circular-     (ii) MOLECULE TYPE: DNA (genomic)-    (iii) HYPOTHETICAL: NO-    (iii) ANTI-SENSE: NO-      (v) FRAGMENT TYPE: N-terminal-     (vi) ORIGINAL SOURCE:#E. coli- and murine originthetic,-    (vii) IMMEDIATE SOURCE:     (B) CLONE: pLISC-SE-     (ix) FEATURE:     (A) NAME/KEY: CDS     (B) LOCATION: 1328..2158#/product= &#34;single chain Fv fragment          (antibody)&#34;#&#34;complete sequence of the pLISC-SE vector&#34;-      (x) PUBLICATION INFORMATION:     (A) AUTHORS: Plack, Pet - #er#Andreas       Plueckthun,     (B) TITLE: Miniantibodies: - # Use of Amphiphatic Helices to          Produce F - #unctional, Flexibly Linked Dimeric Fv#with High Avidity in E. Coli     (C) JOURNAL: Biochemistry     (D) VOLUME: 31     (E) ISSUE: 6     (F) PAGES: 1579-1584     (G) DATE: 1992#1:   (xi) SEQUENCE DESCRIPTION: SEQ ID NO:- ACCCGACACC ATCGAATGGC GCAAAACCTT TCGCGGTATG GCATGATAGC GC - #CCGGAAGA  60- GAGTCAATTC AGGGTGGTGA ATGTGAAACC AGTAACGTTA TACGATGTCG CA - #GAGTATGC 120- CGGTGTCTCT TATCAGACCG TTTCCCGCGT GGTGAACCAG GCCAGCCACG TT - #TCTGCGAA 180- AACGCGGGAA AAAGTGGAAG CGGCGATGGC GGAGCTGAAT TACATTCCCA AC - #CGCGTGGC 240- ACAACAACTG GCGGGCAAAC AGTCGTTGCT GATTGGCGTT GCCACCTCCA GT - #CTGGCCCT 300- GCACGCGCCG TCGCAAATTG TCGCGGCGAT TAAATCTCGC GCCGATCAAC TG - #GGTGCCAG 360- CTGTGTGGTG TCGATGGTAG AACGAAGCGG CGTCGAAGCC TGTAAAGCGG CG - #GTGCACAA 420- TCTTCTCGCG CAACGCGTCA GTGGGCTGAT CATTAACTAT CCGCTGGATG AC - #CAGGATGC 480- CATTGCTGTG GAAGCTGCCT GCACTAATGT TCCGGCGTTA TTTCTTGATG TC - #TCTGACCA 540- GACACCCATC AACAGTATTA TTTTCTCCCA TGAAGACGGT ACGCGACTGG GC - #GTGGAGCA 600- TCTGGTCGCA TTGGGTCACC AGCAAATCGC GCTGTTAGCG GGCCCATTAA GT - #TCTGTCTC 660- GGCGCGTCTG CGTCTGGCTG GCTGGCATAA ATATCTCACT CGCAATCAAA TT - #CAGCCGAT 720- AGCGGAACGG GAAGGCGACT GGAGTGCCAT GTCCGGTTTT CAACAAACCA TG - #CAAATGCT 780- GAATGAGGGC ATCGTTCCCA CTGCGATGCT GGTTGCCAAC GATCAGATGG CG - #CTGGGCGC 840- AATGCGCGCC ATTACCGAGT CCGGGCTGCG CGTTGGTGCG GATGTCTCGG TA - #GTGGGATA 900- CGCAGATACC GAAGACAGCT CATGTTATAT CCCGCCGTTA ACCACCATCA AA - #CAGGATTT 960- TCGCCTGCTG GGGCAAACCA GCGTGGACCG CTTGCTGCAA CTCTCTCAGG GC - #CAGGCGGT1020- GAAGGGCAAT CAGCTGTTGC CCGTCTCACT GGTGAAAAGA AAAACCACCC TG - #GCGCCCAA1080- TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CA - #CGACAGGT1140- TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CT - #CACTCATT1200- AGGCACCCCA GGCTTTACAC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA AT - #TGTGAGCG1260- GATAACAATT TCACACAGGA AACAGCTATG ACCATGATTA CGAATTTCTA GA - #TAACGAGG1320#GTG GCA CTG GCT GGT     1369 ATC GCG ATT GCA#Ala Ile Ala Val Ala Leu Ala Gly#       10- TTC GCT ACC GTA GCG CAG GCC GAA GTT AAA CT - #G GTA GAG TCT GGT GGT1417Phe Ala Thr Val Ala Gln Ala Glu Val Lys Le - #u Val Glu Ser Gly Gly# 30- GGT CTG GTA CAG CCG GGT GGA TCC CTG CGT CT - #G TCT TGC GCT ACC TCA1465Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Le - #u Ser Cys Ala Thr Ser#                 45- GGT TTC ACC TTC TCT GAC TTC TAC ATG GAG TG - #G GTA CGT CAG CCC CCG1513Gly Phe Thr Phe Ser Asp Phe Tyr Met Glu Tr - #p Val Arg Gln Pro Pro#             60- GGT AAA CGT CTC GAG TGG ATC GCA GCT AGC CG - #T AAC AAA GGT AAC AAG1561Gly Lys Arg Leu Glu Trp Ile Ala Ala Ser Ar - #g Asn Lys Gly Asn Lys#         75- TAT ACC ACC GAA TAC AGC GCT TCT GTT AAA GG - #T CGT TTC ATC GTT TCT1609Tyr Thr Thr Glu Tyr Ser Ala Ser Val Lys Gl - #y Arg Phe Ile Val Ser#     90- CGT GAC ACT AGT CAA TCG ATC CTG TAC CTG CA - #G ATG AAT GCA TTG CGT1657Arg Asp Thr Ser Gln Ser Ile Leu Tyr Leu Gl - #n Met Asn Ala Leu Arg#110- GCT GAA GAC ACC GCT ATC TAC TAC TGC GCG CG - #T AAC TAC TAT GGC AGC1705Ala Glu Asp Thr Ala Ile Tyr Tyr Cys Ala Ar - #g Asn Tyr Tyr Gly Ser#               125- ACT TGG TAC TTC GAC GTT TGG GGT GCA GGT AC - #C ACC GTT ACC GTT TCT1753Thr Trp Tyr Phe Asp Val Trp Gly Ala Gly Th - #r Thr Val Thr Val Ser#           140- TCT GGT GGT GGT GGT TCT GGT GGT GGT GGT TC - #T GGT GGT GGT GGT TCT1801Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Se - #r Gly Gly Gly Gly Ser#       155- GAT ATC GTT ATG ACC CAG TCT CCG AGC TCT CT - #G TCT GTA TCT GCA GGT1849Asp Ile Val Met Thr Gln Ser Pro Ser Ser Le - #u Ser Val Ser Ala Gly#   170- GAA CGT GTT ACC ATG TCT TGC AAA TCT TCT CA - #G TCT CTG CTG AAC TCT1897Glu Arg Val Thr Met Ser Cys Lys Ser Ser Gl - #n Ser Leu Leu Asn Ser175                 1 - #80                 1 - #85                 1 -#90- GGT AAC CAG AAA AAC TTC CTG GCG TGG TAT CA - #G CAA AAG CCT GGC CAA1945Gly Asn Gln Lys Asn Phe Leu Ala Trp Tyr Gl - #n Gln Lys Pro Gly Gln#               205- CCG CCG AAA CTG CTG ATC TAC GGT GCG TCG AC - #C CGT GAA TCT GGT GTT1993Pro Pro Lys Leu Leu Ile Tyr Gly Ala Ser Th - #r Arg Glu Ser Gly Val#           220- CCG GAC CGT TTT ACC GGT AGC GGT AGC GGT AC - #C GAC TTC ACT CTG ACC2041Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Th - #r Asp Phe Thr Leu Thr#       235- ATC TCT TCT GTA CAG GCT GAA GAT CTG GCT GT - #T TAC TAC TGT CAA AAC2089Ile Ser Ser Val Gln Ala Glu Asp Leu Ala Va - #l Tyr Tyr Cys Gln Asn#   250- GAC CAC TCT TAC CCG CTG ACC TTT GGC GCC GG - #C ACC AAA CTG GAA CTG2137Asp His Ser Tyr Pro Leu Thr Phe Gly Ala Gl - #y Thr Lys Leu Glu Leu255                 2 - #60                 2 - #65                 2 -#70- AAG CGC GCT AAC GGT GAA TTC TGATAAGCTT GACCTGTGA - #A GTGAAAAATG2188Lys Arg Ala Asn Gly Glu Phe           275- GCGCACATTG TGCGACATTT TTTTTGTCTG CCGTTTACCG CTACTGCGTC AC - #GGATCCCC2248- ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AG - #CGTGACCG2308- CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TT - #TCTCGCCA2368- CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGCAT CCCTTTAGGG TT - #CCGATTTA2428- GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA CG - #TAGTGGGC2488- CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TT - #TAATAGTG2548- GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT TT - #TGATTTAT2608- AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CA - #AAAATTTA2668- ACGCGAATTT TAACAAAATA TTAACGTTTA CAATTTCAGG TGGCACTTTT CG - #GGGAAATG2728- TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CC - #GCTCATGA2788- GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AG - #TATTCAAC2848- ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TT - #TGCTCACC2908- CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GT - #GGGTTACA2968- TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GA - #ACGTTTTC3028- CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT AT - #TGACGCCG3088- GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GA - #GTACTCAC3148- CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AG - #TGCTGCCA3208- TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GG - #ACCGAAGG3268- AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CG - #TTGGGAAC3328- CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GT - #AGCAATGG3388- CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CG - #GCAACAAT3448- TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GC - #CCTTCCGG3508- CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GG - #TATCATTG3568- CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG AC - #GGGGAGTC3628- AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CT - #GATTAAGC3688- ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AA - #ACTTCATT3748- TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AA - #AATCCCTT3808- AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GG - #ATCTTCTT3868- GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CC - #GCTACCAG3928- CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA AC - #TGGCTTCA3988- GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CA - #CCACTTCA4048- AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GT - #GGCTGCTG4108- CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CC - #GGATAAGG4168- CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CG - #AACGACCT4228- ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CC - #CGAAGGGA4288- GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC AC - #GAGGGAGC4348- TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CT - #CTGACTTG4408- AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GC - #CAGCAACG4468#              4515CCTG GCCTTTTGCT GGCCTTTTGC TCACATG- (2) INFORMATION FOR SEQ ID NO: 2:-      (i) SEQUENCE CHARACTERISTICS:#acids    (A) LENGTH: 277 amino     (B) TYPE: amino acid     (D) TOPOLOGY: linear-     (ii) MOLECULE TYPE: protein#2:   (xi) SEQUENCE DESCRIPTION: SEQ ID NO:- Met Lys Lys Thr Ala Ile Ala Ile Ala Val Al - #a Leu Ala Gly Phe Ala#                 15- Thr Val Ala Gln Ala Glu Val Lys Leu Val Gl - #u Ser Gly Gly Gly Leu#             30- Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cy - #s Ala Thr Ser Gly Phe#         45- Thr Phe Ser Asp Phe Tyr Met Glu Trp Val Ar - #g Gln Pro Pro Gly Lys#     60- Arg Leu Glu Trp Ile Ala Ala Ser Arg Asn Ly - #s Gly Asn Lys Tyr Thr# 80- Thr Glu Tyr Ser Ala Ser Val Lys Gly Arg Ph - #e Ile Val Ser Arg Asp#                 95- Thr Ser Gln Ser Ile Leu Tyr Leu Gln Met As - #n Ala Leu Arg Ala Glu#           110- Asp Thr Ala Ile Tyr Tyr Cys Ala Arg Asn Ty - #r Tyr Gly Ser Thr Trp#       125- Tyr Phe Asp Val Trp Gly Ala Gly Thr Thr Va - #l Thr Val Ser Ser Gly#   140- Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gl - #y Gly Gly Ser Asp Ile145                 1 - #50                 1 - #55                 1 -#60- Val Met Thr Gln Ser Pro Ser Ser Leu Ser Va - #l Ser Ala Gly Glu Arg#               175- Val Thr Met Ser Cys Lys Ser Ser Gln Ser Le - #u Leu Asn Ser Gly Asn#           190- Gln Lys Asn Phe Leu Ala Trp Tyr Gln Gln Ly - #s Pro Gly Gln Pro Pro#       205- Lys Leu Leu Ile Tyr Gly Ala Ser Thr Arg Gl - #u Ser Gly Val Pro Asp#   220- Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Ph - #e Thr Leu Thr Ile Ser225                 2 - #30                 2 - #35                 2 -#40- Ser Val Gln Ala Glu Asp Leu Ala Val Tyr Ty - #r Cys Gln Asn Asp His#               255- Ser Tyr Pro Leu Thr Phe Gly Ala Gly Thr Ly - #s Leu Glu Leu Lys Arg#           270- Ala Asn Gly Glu Phe   275- (2) INFORMATION FOR SEQ ID NO: 3:-      (i) SEQUENCE CHARACTERISTICS:#pairs    (A) LENGTH: 151 base     (B) TYPE: nucleic acid     (C) STRANDEDNESS: double     (D) TOPOLOGY: linear-     (ii) MOLECULE TYPE: DNA (genomic)-    (iii) HYPOTHETICAL: NO-    (iii) ANTI-SENSE: NO-      (v) FRAGMENT TYPE: N-terminal-     (vi) ORIGINAL SOURCE:#(deduced from yeast + mousehetic          sequences)-     (ix) FEATURE:     (A) NAME/KEY: CDS     (B) LOCATION: 1..138#/product= &#34;intercalating peptide&#34;#&#34;Gene cassette of intercalating          GCN4-leucine - # zipper&#34;-     (ix) FEATURE:     (A) NAME/KEY: misc.sub.-- - #feature     (B) LOCATION: 10..39#/product= &#34;Immunoglobulin Joining          Region&#34;#&#34;IgG3-hinge&#34;  /note=-     (ix) FEATURE:     (A) NAME/KEY: misc.sub.-- - #feature     (B) LOCATION: 40..138#/product= &#34;GCN4-zipper&#34;RMATION:#3:   (xi) SEQUENCE DESCRIPTION: SEQ ID NO:- GGT GAA TTC CCC AAA CCT AGT ACT CCC CCT GG - #C AGC AGC CGC ATG AAA  48Gly Glu Phe Pro Lys Pro Ser Thr Pro Pro Gl - #y Ser Ser Arg Met Lys#                 15- CAG CTG GAA GAT AAA GTT GAA GAG CTT CTT TC - #G AAA AAC TAC CAC CTC  96Gln Leu Glu Asp Lys Val Glu Glu Leu Leu Se - #r Lys Asn Tyr His Leu#             30- GAA AAT GAA GTT GCG CGC CTC AAA AAA CTT GT - #T GGT GAA CGC# 138Glu Asn Glu Val Ala Arg Leu Lys Lys Leu Va - #l Gly Glu Arg#         45#     151- (2) INFORMATION FOR SEQ ID NO: 4:-      (i) SEQUENCE CHARACTERISTICS:#acids    (A) LENGTH: 46 amino     (B) TYPE: amino acid     (D) TOPOLOGY: linear-     (ii) MOLECULE TYPE: protein#4:   (xi) SEQUENCE DESCRIPTION: SEQ ID NO:- Gly Glu Phe Pro Lys Pro Ser Thr Pro Pro Gl - #y Ser Ser Arg Met Lys#                 15- Gln Leu Glu Asp Lys Val Glu Glu Leu Leu Se - #r Lys Asn Tyr His Leu#             30- Glu Asn Glu Val Ala Arg Leu Lys Lys Leu Va - #l Gly Glu Arg#         45- (2) INFORMATION FOR SEQ ID NO: 5:-      (i) SEQUENCE CHARACTERISTICS:#pairs    (A) LENGTH: 181 base     (B) TYPE: nucleic acid     (C) STRANDEDNESS: double     (D) TOPOLOGY: linear-     (ii) MOLECULE TYPE: DNA (genomic)-    (iii) HYPOTHETICAL: NO-    (iii) ANTI-SENSE: NO-      (v) FRAGMENT TYPE: N-terminal-     (vi) ORIGINAL SOURCE:     (A) ORGANISM: synthetic-     (ix) FEATURE:     (A) NAME/KEY: CDS     (B) LOCATION: 1..150#/product= &#34;intercalating peptide&#34;#&#34;Gene casette encoding intercalating          antiparallel - # helix-turn-helix&#34;-     (ix) FEATURE:     (A) NAME/KEY: misc.sub.-- - #feature     (B) LOCATION: 10..39#/product= &#34;Immunoglobulin Joining          Region&#34;#&#34;IgG3-hinge&#34;  /note=-     (ix) FEATURE:     (A) NAME/KEY: misc.sub.-- - #feature     (B) LOCATION: 40..87#/product= &#34;helix peptide&#34;ATION:#&#34;bundle-helix A&#34;ote=-     (ix) FEATURE:     (A) NAME/KEY: misc.sub.-- - #feature     (B) LOCATION: 88..150#/product= &#34;helix peptide&#34;ATION:#&#34;bundle-helix B&#34;ote=#5:   (xi) SEQUENCE DESCRIPTION: SEQ ID NO:- GGT GAA TTC CCC AAA CCT AGC ACC CCC CCT GG - #C AGC AGT GGT GAA CTG  48Gly Glu Phe Pro Lys Pro Ser Thr Pro Pro Gl - #y Ser Ser Gly Glu Leu#                 15- GAA GAG CTG CTT AAG CAT CTT AAA GAA CTT CT - #G AAG GGC CCC CGC AAA  96Glu Glu Leu Leu Lys His Leu Lys Glu Leu Le - #u Lys Gly Pro Arg Lys#             30- GGC GAA CTC GAG GAA CTG CTG AAA CAT CTG AA - #G GAG CTG CTT AAA GGT 144Gly Glu Leu Glu Glu Leu Leu Lys His Leu Ly - #s Glu Leu Leu Lys Gly#         45#     181          T GACCTGTGAA GTGAAAAAAT GGlu Phe50- (2) INFORMATION FOR SEQ ID NO: 6:-      (i) SEQUENCE CHARACTERISTICS:#acids    (A) LENGTH: 50 amino     (B) TYPE: amino acid     (D) TOPOLOGY: linear-     (ii) MOLECULE TYPE: protein#6:   (xi) SEQUENCE DESCRIPTION: SEQ ID NO:- Gly Glu Phe Pro Lys Pro Ser Thr Pro Pro Gl - #y Ser Ser Gly Glu Leu#                 15- Glu Glu Leu Leu Lys His Leu Lys Glu Leu Le - #u Lys Gly Pro Arg Lys#             30- Gly Glu Leu Glu Glu Leu Leu Lys His Leu Ly - #s Glu Leu Leu Lys Gly#         45- Glu Phe50- (2) INFORMATION FOR SEQ ID NO: 7:-      (i) SEQUENCE CHARACTERISTICS:#pairs    (A) LENGTH: 180 base     (B) TYPE: nucleic acid     (C) STRANDEDNESS: double     (D) TOPOLOGY: linear-     (ii) MOLECULE TYPE: DNA (genomic)-    (iii) HYPOTHETICAL: NO-    (iii) ANTI-SENSE: NO-      (v) FRAGMENT TYPE: N-terminal-     (vi) ORIGINAL SOURCE:#deduced from human and murinetic,          sequences-     (ix) FEATURE:     (A) NAME/KEY: CDS     (B) LOCATION: 1..159#/product= &#34;intercalating peptide&#34;#&#34;gene cassette encoding intercalating#and IgG3-hinge reg...&#34;er-     (ix) FEATURE:     (A) NAME/KEY: misc.sub.-- - #feature     (B) LOCATION: 10..39#/product= &#34;Immunoglobulin Joining          Region&#34;#&#34;IgG3-hinge region (mouse)&#34;-     (ix) FEATURE:     (A) NAME/KEY: misc.sub.-- - #feature     (B) LOCATION: 40..159#/product= &#34;jun-zipper&#34;ORMATION:#7:   (xi) SEQUENCE DESCRIPTION: SEQ ID NO:- GGT GAA TTC CCC AAA CCT AGT ACT CCC CCT GG - #C AGC AGC CGT ATC GCT  48Gly Glu Phe Pro Lys Pro Ser Thr Pro Pro Gl - #y Ser Ser Arg Ile Ala#                 15- CGT CTC GAG GAA AAA GTT AAA ACC CTG AAA GC - #T CAG AAC TCC GAA CTG  96Arg Leu Glu Glu Lys Val Lys Thr Leu Lys Al - #a Gln Asn Ser Glu Leu#             30- GCT TCC ACC GCT AAC ATG CTG CGT GAA CAG GT - #T GCT CAG CTG AAA CAG 144Ala Ser Thr Ala Asn Met Leu Arg Glu Gln Va - #l Ala Gln Leu Lys Gln#         45#      180         AC TGATAAGCTT GACCTGTGAA GLys Val Met Asn Tyr50- (2) INFORMATION FOR SEQ ID NO: 8:-      (i) SEQUENCE CHARACTERISTICS:#acids    (A) LENGTH: 53 amino     (B) TYPE: amino acid     (D) TOPOLOGY: linear-     (ii) MOLECULE TYPE: protein#8:   (xi) SEQUENCE DESCRIPTION: SEQ ID NO:- Gly Glu Phe Pro Lys Pro Ser Thr Pro Pro Gl - #y Ser Ser Arg Ile Ala#                 15- Arg Leu Glu Glu Lys Val Lys Thr Leu Lys Al - #a Gln Asn Ser Glu Leu#             30- Ala Ser Thr Ala Asn Met Leu Arg Glu Gln Va - #l Ala Gln Leu Lys Gln#         45- Lys Val Met Asn Tyr50- (2) INFORMATION FOR SEQ ID NO: 9:-      (i) SEQUENCE CHARACTERISTICS:#pairs    (A) LENGTH: 180 base     (B) TYPE: nucleic acid     (C) STRANDEDNESS: double     (D) TOPOLOGY: linear-     (ii) MOLECULE TYPE: DNA (genomic)-    (iii) HYPOTHETICAL: NO-    (iii) ANTI-SENSE: NO-      (v) FRAGMENT TYPE: N-terminal-     (vi) ORIGINAL SOURCE:#deduced from human and murinetic,          sequences-     (ix) FEATURE:     (A) NAME/KEY: CDS     (B) LOCATION: 1..159#/product= &#34;intercalating peptide&#34;#&#34;gene cassette encoding intercalating#and IgG3-hinge&#34;os-zipper-     (ix) FEATURE:     (A) NAME/KEY: misc.sub.-- - #feature     (B) LOCATION: 10..39#/product= &#34;Immunoglobulin Joining          Region&#34;#&#34;IgG3-hinge (mouse)&#34;-     (ix) FEATURE:     (A) NAME/KEY: misc.sub.-- - #feature     (B) LOCATION: 40..159#/product= &#34;fos-zipper&#34;ORMATION:#9:   (xi) SEQUENCE DESCRIPTION: SEQ ID NO:- GGT GAA TTC CCC AAA CCT AGT ACT CCC CCT GG - #C AGC AGC CTG ACC GAC  48Gly Glu Phe Pro Lys Pro Ser Thr Pro Pro Gl - #y Ser Ser Leu Thr Asp#                 15- ACC CTG CAG GCT GAA ACC GAC CAG CTG GAA GA - #C AAA AAA TCC GCT CTG  96Thr Leu Gln Ala Glu Thr Asp Gln Leu Glu As - #p Lys Lys Ser Ala Leu#             30- CAG ACC GAA ATC GCT AAC CTG CTG AAA GAA AA - #A GAA AAA CTG GAA TTT 144Gln Thr Glu Ile Ala Asn Leu Leu Lys Glu Ly - #s Glu Lys Leu Glu Phe#         45#      180         AC TGATAAGCTT GACCTGTGAA GIle Leu Ala Ala Tyr50- (2) INFORMATION FOR SEQ ID NO: 10:-      (i) SEQUENCE CHARACTERISTICS:#acids    (A) LENGTH: 53 amino     (B) TYPE: amino acid     (D) TOPOLOGY: linear-     (ii) MOLECULE TYPE: protein#10:  (xi) SEQUENCE DESCRIPTION: SEQ ID NO:- Gly Glu Phe Pro Lys Pro Ser Thr Pro Pro Gl - #y Ser Ser Leu Thr Asp#                 15- Thr Leu Gln Ala Glu Thr Asp Gln Leu Glu As - #p Lys Lys Ser Ala Leu#             30- Gln Thr Glu Ile Ala Asn Leu Leu Lys Glu Ly - #s Glu Lys Leu Glu Phe#         45- Ile Leu Ala Ala Tyr50- (2) INFORMATION FOR SEQ ID NO: 11:-      (i) SEQUENCE CHARACTERISTICS:#pairs    (A) LENGTH: 180 base     (B) TYPE: nucleic acid     (C) STRANDEDNESS: double     (D) TOPOLOGY: linear-     (ii) MOLECULE TYPE: DNA (genomic)-    (iii) HYPOTHETICAL: NO-    (iii) ANTI-SENSE: NO-      (v) FRAGMENT TYPE: N-terminal-     (vi) ORIGINAL SOURCE:#deduced from human sequencesetic,-     (ix) FEATURE:     (A) NAME/KEY: CDS     (B) LOCATION: 1..147#/product= &#34;intercalating peptide&#34;#&#34;gene cassete encoding intercalating#and linker&#34;   jun-zipper-     (ix) FEATURE:     (A) NAME/KEY: misc.sub.-- - #feature     (B) LOCATION: 10..27#/product= &#34;synthetic linker&#34;ON:-     (ix) FEATURE:     (A) NAME/KEY: misc.sub.-- - #feature     (B) LOCATION: 28..147#/product= &#34;jun-zipper&#34;ORMATION:#11:  (xi) SEQUENCE DESCRIPTION: SEQ ID NO:- GGT GAA TTC CCG TCT GGT AAC GAA GCT CGT AT - #C GCT CGT CTC GAG GAA  48Gly Glu Phe Pro Ser Gly Asn Glu Ala Arg Il - #e Ala Arg Leu Glu Glu#                 15- AAA GTT AAA ACC CTG AAA GCT CAG AAC TCC GA - #A CTG GCT TCC ACC GCT  96Lys Val Lys Thr Leu Lys Ala Gln Asn Ser Gl - #u Leu Ala Ser Thr Ala#             30- AAC ATG CTG CGT GAA CAG GTT GCT CAG CTG AA - #A CAG AAA GTT ATG AAC 144Asn Met Leu Arg Glu Gln Val Ala Gln Leu Ly - #s Gln Lys Val Met Asn#         45#      180         CCTGTGAA GTGAAAAATG GCGTyr- (2) INFORMATION FOR SEQ ID NO: 12:-      (i) SEQUENCE CHARACTERISTICS:#acids    (A) LENGTH: 49 amino     (B) TYPE: amino acid     (D) TOPOLOGY: linear-     (ii) MOLECULE TYPE: protein#12:  (xi) SEQUENCE DESCRIPTION: SEQ ID NO:- Gly Glu Phe Pro Ser Gly Asn Glu Ala Arg Il - #e Ala Arg Leu Glu Glu#                 15- Lys Val Lys Thr Leu Lys Ala Gln Asn Ser Gl - #u Leu Ala Ser Thr Ala#             30- Asn Met Leu Arg Glu Gln Val Ala Gln Leu Ly - #s Gln Lys Val Met Asn#         45- Tyr- (2) INFORMATION FOR SEQ ID NO: 13:-      (i) SEQUENCE CHARACTERISTICS:#pairs    (A) LENGTH: 180 base     (B) TYPE: nucleic acid     (C) STRANDEDNESS: double     (D) TOPOLOGY: linear-     (ii) MOLECULE TYPE: DNA (genomic)-    (iii) HYPOTHETICAL: NO-    (iii) ANTI-SENSE: NO-      (v) FRAGMENT TYPE: N-terminal-     (vi) ORIGINAL SOURCE:#deduced from human sequencesetic,-     (ix) FEATURE:     (A) NAME/KEY: CDS     (B) LOCATION: 1..147#/product= &#34;intercalating peptide&#34;#&#34;gene cassette encoding intercalating#and linker&#34;   fos-zipper-     (ix) FEATURE:     (A) NAME/KEY: misc.sub.-- - #feature     (B) LOCATION: 10..27#/product= &#34;synthetic linker&#34;ON:-     (ix) FEATURE:     (A) NAME/KEY: misc.sub.-- - #feature     (B) LOCATION: 28..147#/product= &#34;fos-zipper&#34;ORMATION:#13:  (xi) SEQUENCE DESCRIPTION: SEQ ID NO:- GGT GAA TTC GGT CCG TCT GGT AAC GAA CTG AC - #C GAC ACC CTG CAG GCT  48Gly Glu Phe Gly Pro Ser Gly Asn Glu Leu Th - #r Asp Thr Leu Gln Ala#                 15- GAA ACC GAC CAG CTG GAA GAC AAA AAA TCC GC - #T CTG CAG ACC GAA ATC  96Glu Thr Asp Gln Leu Glu Asp Lys Lys Ser Al - #a Leu Gln Thr Glu Ile#             30- GCT AAC CTG CTG AAA GAA AAA GAA AAA CTG GA - #A TTT ATC CTG GCT GCT 144Ala Asn Leu Leu Lys Glu Lys Glu Lys Leu Gl - #u Phe Ile Leu Ala Ala#         45#      180         CCTGTGAA GTGAAAAATG GCGTyr- (2) INFORMATION FOR SEQ ID NO: 14:-      (i) SEQUENCE CHARACTERISTICS:#acids    (A) LENGTH: 49 amino     (B) TYPE: amino acid     (D) TOPOLOGY: linear-     (ii) MOLECULE TYPE: protein#14:  (xi) SEQUENCE DESCRIPTION: SEQ ID NO:- Gly Glu Phe Gly Pro Ser Gly Asn Glu Leu Th - #r Asp Thr Leu Gln Ala#                 15- Glu Thr Asp Gln Leu Glu Asp Lys Lys Ser Al - #a Leu Gln Thr Glu Ile#             30- Ala Asn Leu Leu Lys Glu Lys Glu Lys Leu Gl - #u Phe Ile Leu Ala Ala#         45- Tyr__________________________________________________________________________