Abstract:
Culture medium composition for fungi and yeast, preparation method and uses of the composition. The culture medium composition is employed for analyzing microorganisms contamination in the food industry, preferably in the cola beverages industry, wherein the composition comprises between 4.5 and 5.5 g/l of monobasic potassium phosphate, between 0.5 and 1.5 g/l of ammonium chloride, between 0.5 and 1.5 g/l of heptahydrate magnesium sulfate, between 30.0 and 50.0 g/l of D(+) saccharose, and water.

Description:
BACKGROUND OF THE INVENTION  
         [0001]    1. Field of the Invention  
           [0002]    The present invention relates to a culture medium or composition for the culturing, detection, counting and identification of microorganisms and more particularly the invention refers to a culture medium or composition for detecting and counting fungi and/or yeast in any kind of installations, most preferably installations employed in the food industry, with the composition basically comprising a solution of monobasic potassium phosphate, ammonium chloride, heptahydrate magnesium sulfate, D(+) saccharose, and water.  
           [0003]    While specific reference will be made in this specification to the application of the inventive medium in the food industry, it will be apparent to any person skilled in the art that the invention can be applied into any field where a control of microorganisms, or any other agent such as undesired or pathogenic agent, is to be carried out.  
           [0004]    2. Description of the Prior Art  
           [0005]    The food industry, particularly the industry of producing cola beverages, requires of control tests allowing the evaluation of contaminating fungi and yeast that may be present in all and each of the production stages. To date such evaluation is carried out by culturing, in culture mediums, samples taken from the industrial devices or products, and by further analyzing the growing of fungi and/or yeast colonies. To this purpose several culture mediums, such as the Sartorius® dehydrated medium for detecting fungi and yeast and the Bioplast® liquid medium, are employed, the compositions of which mediums being as follows:  
           [0006]    Bioplast® liquid medium:  
           [0007]    Yeast extract 9.0 g/l±0.5 g/l.  
           [0008]    Dextrose 50.0 g/l±0.5 g/l.  
           [0009]    Digested pancreatic casein 5.0 g/l±0.5 g/l.  
           [0010]    Digested peptides of animal tissue 5.0 g/l±0.5 g/l.  
           [0011]    Final pH 4.6±0.2.  
           [0012]    Sartorius® dehydrated culture medium:  
           [0013]    Yeast extract 9.0 g/l.  
           [0014]    Dextrose 50.0 g/l.  
           [0015]    Peptone 10.0 g/l.  
           [0016]    Magnesium sulfate 2.1 g/l.  
           [0017]    Diastase 0.05 g/l.  
           [0018]    Thiamine 0.05 g/l.  
           [0019]    Final pH 4.6.  
           [0020]    In the food or beverages production stages a large number of evaluations must be made to determine the presence of fungi and yeast, this resulting in high costs for the industry using such culture mediums. There is a need, therefore, of having culture medium compositions that would be cheaper but capable of having an efficiency for evaluating colonies that is the same or higher than the culture mediums usually employed in the industry.  
         SUMMARY OF THE INVENTION  
         [0021]    It is therefore one object of the present invention to provide a culture medium comprising:  
           [0022]    i. between about 4.5 g/l and about 5.5 g/l of monobasic potassium phosphate;  
           [0023]    ii. between about 0.5 g/l and about 1.5 g/l of ammonium chloride;  
           [0024]    iii. between about 0.5 g/l and about 1.5 g/l of heptahydrate magnesium sulfate;  
           [0025]    iv. between about 30.0 g/l and about 50.0 g/l of D(+) saccharose, and  
           [0026]    v. water, wherein the pH of the medium may be between 4.5 and 5.5.  
           [0027]    It is even another object of the present invention to provide a method of preparing a culture medium comprising between about 4.5 g/l and about 5.5 g/l of monobasic potassium phosphate; between about 0.5 g/l and about 1.5 g/l of ammonium chloride; between about 0.5 g/l and about 1.5 g/l of heptahydrate magnesium sulfate; between about 30.0 g/l and about 50.0 g/l of D(+) saccharose, and water, wherein the method comprises the steps of:  
           [0028]    a. obtaining a solution by dissolving in ultra pure water, under agitation, the monobasic potassium phosphate, the ammonium chloride, the heptahydrate magnesium sulfate and the saccharose;  
           [0029]    b. adjusting the pH of the solution obtained in step a. to 5.0, and  
           [0030]    c. sterilizing the solution and conserving the solution at a temperature of 4° C.  
           [0031]    It is still another object of the present invention to provide a culture medium composition for detecting fungi and/or yeast in installations, the composition comprising monobasic potassium phosphate, ammonium chloride, heptahydrate magnesium sulfate, D(+) saccharose, and water.  
           [0032]    It is a further object of the present invention to provide a culture medium composition for detecting fungi and/or yeast in several installations, preferably food and/or beverages industry installations, wherein the fungi and yeast are selected from the group consisting of  Basillus Subtilis, Candida Albicans, Saccharomyces Cerevisiae, Saccharomyces Uvarum, Rhodotorula Rubra, Penicillium Camemberti, Aspergillus Niger, Trychophyton Ajelloi  and  Geotrichum Candidum.    
           [0033]    It is even a further object of the present invention to provide a method of preparing a culture medium comprising monobasic potassium phosphate, ammonium chloride, heptahydrate magnesium sulfate, D(+) saccharose, and water, wherein the method comprises the steps of:  
           [0034]    a. obtaining a solution by dissolving in ultra pure water, under agitation, the monobasic potassium phosphate, the ammonium chloride, the heptahydrate magnesium sulfate and the saccharose;  
           [0035]    b. adjusting the pH of the solution obtained in step a. to 5.0, and  
           [0036]    c. sterilizing the solution and conserving the solution at a temperature of 4° C. 
       
    
    
     BRIEF DESCRIPTION OF THE DRAWINGS  
       [0037]    The present invention is illustrated by way of example in the following drawings wherein:  
         [0038]    [0038]FIG. 1 a  is a photo showing the development of microorganisms colonies of one sample after 3 days of culturing by employing the culture medium composition of the invention, and  
         [0039]    [0039]FIG. 1 b  is a photo showing the development of microorganisms colonies of one sample after 3 days of culturing by employing the culture medium Sartorius®. 
     
    
     DESCRIPTION OF THE PREFERRED EMBODIMENTS  
       [0040]    The inventive composition may be employed for evaluating, in the food industry, the presence of contaminating fungi and yeast. In a preferred embodiment the composition of the invention is employed for growing and counting fungi and yeast colonies in the cola beverages industry, for instance by taking samples in each of the production stages or by taking samples from the products employed in the process for elaborating the beverages.  
         [0041]    Any expert in the art will understand that the culture medium composition of the invention may be used for culturing a variety of organisms, such as fungi and yeast, in a number of different industrial processes including processes for elaborating beverages, food products and other products where the presence of said organisms must be kept under control.  
         [0042]    With the purpose of testing the effectiveness of the inventive composition as compared to other commercial culture mediums, a number of fungi and yeast cultures have been prepared from different dilutions of a mother solution containing microorganisms obtained from isolated colonies.  
         [0043]    The test results are shown in the following Table:  
                                                     TABLE 1                                           Composition                   of the           Sartorius ®   Bioplast ®   invention                                        Target   0 ufc   0 ufc   0 ufc           Concentrated   TNTC   TNTC   TNTC           1/10 2     TNTC   TNTC   TNTC           1/10 4     25 ufc    TNTC   TNTC           1/10 6     0 ufc   106 ufc    109 ufc            1/10 8     0 ufc   4 ufc   6 ufc           1/10 10     0 ufc   0 ufc   0 ufc           1/10 12     0 ufc   0 ufc   0 ufc           1/10 14     0 ufc   0 ufc   0 ufc           1/10 16     0 ufc   0 ufc   0 ufc           1/10 18     0 ufc   0 ufc   0 ufc           1/10 20     0 ufc   0 ufc   0 ufc                      
 
         [0044]    To the purpose of the present specification the term “TNTC” means “too much colonies to be counted”.  
         [0045]    As may be seen from the above Table the inventive composition complied with exigent requirements for the culturing tests of microorganisms present in the cola beverages production plants, thus permitting the proper growing not only of fungi but also of yeast, and also providing a proper medium insofar as the nutrients availability, humidity and pH.  
         [0046]    While the medium Sartorius® did not provide a response to samples having a very low contamination, the culture medium Bioplast® and the composition of the invention did not have obstacles in evidencing the contamination that was present in the solution at low concentrations.  
         [0047]    It was also observed that the colonies grown in the inventive medium had dimensions smaller than the ones of the other mediums; this feature permitted to more easily count the colonies in high contamination levels because the colonies did not form an homogeneous layer on the culture membrane (FIGS. 1 a  and  1   b ).  
         [0048]    The colonies counted in the analyzed plates were similar and almost the same for three tests.  
         [0049]    Before carrying out the above mentioned tests, a number of evaluations consisting of comparing the culture mediums Sartorius®, Bioplast® and the one of the invention have been made with samples taken from filling machines in the production lines, thus guaranteeing in a larger extent the efficiency and quality of the inventive culture medium composition.  
         [0050]    The tests results are shown in the following Table.  
                                             TABLE 2                                       Composition           Medium   of the           Sartorius ®   invention                                        Valve n° 15   00 ufc   04 ufc           Valve n° 30   00 ufc   08 ufc           Valve n° 45   00 ufc   05 ufc           Valve n° 60   00 ufc   07 ufc                      
 
         [0051]    It is apparent from the above results that the culture composition of the invention provides a response that is better than the one of the commercial medium in the detection of microorganisms when the same are present in a very low concentration.  
         [0052]    When the composition of the invention has been compared to the commercial culture mediums Sartorius® and Bioplast® in connection to the response to high and low concentrations of fungi the following results have been found:  
                                             TABLE 3                               Contami-           Sample   nation   Culture Medium            No.   degree   Sartorius ®   Bioplast ®   Invention               1   High   TNTC   TNTC   TNTC       2   High   TNTC   TNTC   TNTC       3   High   TNTC   TNTC   TNTC       4   High   TNTC   TNTC   TNTC       5   High   TNTC   TNTC   TNTC       6   High   TNTC   TNTC   TNTC       7   Low   05 ufc   04 ufc   04 ufc                  
 
         [0053]    From the results in the above Table 3 it may be seen that either in samples with low microbial concentrations as well as in samples with excessive contamination the inventive composition provides a response similar to the other tested mediums, comparatively giving an equivalent amount of ufc.  
         [0054]    A particular feature of this new culture medium composition is the size that the colonies have when growing in the inventive medium. For contaminations having values of about 300-400 ufc, the colonies developed in mediums Sartorius® and Bioplast® have a considerable size or dimension producing a homogeneous and continuous layer formed in the medium thus preventing the colonies from being counted. For the same amount of colonies the composition of the invention allows a correct counting of colonies because the colonies are of smaller size and are spaced apart from each other.  
         [0055]    The above mentioned test results show that the composition of the invention is effective for the counting of fungi and yeast commonly found in the food industry and more preferably for the counting of fungi and yeast that are present in the production lines of cola beverages.  
         [0056]    The 500 comparative tests in the following Table show that the composition of the invention has a fungi and yeast detection level that is equal or superior to the one of available commercial culture mediums.  
                                                                             TABLE 4                       Test               Sample   Inventive   Bioplast   Sartorius       #   Date   Sector   Equipment   Type   Composition   ®   ®                                1   8 Nov. 2001   Unloading   Transport   Fructose   01 ufc   —   00 ufc       2   8 Nov. 2001   Unloading   Transport   Fructose   00 ufc   —   01 ufc       3   8 Nov. 2001   Unloading   Transport   Fructose   02 ufc   —   03 ufc       4   8 Nov. 2001   Unloading   Transport   Fructose   02 ufc   —   02 ufc       5   8 Nov. 2001   Unloading   Transport   Fructose   01 ufc   —   01 ufc       6   8 Nov. 2001   Unloading   Transport   Fructose   00 ufc   —   02 ufc       7   9 Nov. 2001   Bottling   Filling   Sanitation   05 ufc   —   08 ufc                   Valve   Water       8   9 Nov. 2001   Bottling   Filling   Sanitation   02 ufc   —   06 ufc                   Valve   Water       9   9 Nov. 2001   Bottling   Filling   Sanitation   10 ufc   —   03 ufc                   Valve   Water       10   9 Nov. 2001   Bottling   Filling   Sanitation   07 ufc   —   09 ufc                   Valve   Water       11   10 Nov. 2001   Bottling   Filling   Sanitation   10 ufc   —   09 ufc                   Valve   Water       12   10 Nov. 2001   Bottling   Filling   Sanitation   08 ufc   —   07 ufc                   Valve   Water       13   10 Nov. 2001   Bottling   Filling   Sanitation   08 ufc   —   05 ufc                   Valve   Water       14   10 Nov. 2001   Bottling   Filling   Sanitation   11 ufc   —   13 ufc                   Valve   Water       15   11 Nov. 2001   Quality   Laboratory   Prepared   23 ufc   —   87 ufc               Control       16   11 Nov. 2001   Quality   Laboratory   Prepared   62 ufc   —   117 ufc               Control       17   11 Nov. 2001   Quality   Laboratory   Prepared   56 ufc   —   99 ufc               Control       18   11 Nov. 2001   Quality   Laboratory   Prepared   26 ufc   —   88 ufc               Control       19   11 Nov. 2001   Quality   Laboratory   Prepared   54 ufc   —   83 ufc               Control       20   20 Nov. 2001   Quality   Laboratory   Prepared   TNTC   TNTC   TNTC               Control       21   20 Nov. 2001   Quality   Laboratory   Prepared   TNTC   TNTC   TNTC               Control       22   20 Nov. 2001   Quality   Laboratory   Prepared   TNTC   TNTC   TNTC               Control       23   20 Nov. 2001   Quality   Laboratory   Prepared   TNTC   TNTC   TNTC               Control       24   20 Nov. 2001   Quality   Laboratory   Prepared   TNTC   TNTC   TNTC               Control       25   21 Nov. 2001   Quality   Laboratory   Prepared   TNTC   TNTC   TNTC               Control       26   21 Nov. 2001   Quality   Laboratory   Prepared   TNTC   TNTC   TNTC               Control       27   21 Nov. 2001   Quality   Laboratory   Prepared   TNTC   TNTC   TNTC               Control       28   21 Nov. 2001   Quality   Laboratory   Prepared   TNTC   TNTC   TNTC               Control       29   21 Nov. 2001   Quality   Laboratory   Prepared   TNTC   TNTC   TNTC               Control       30   23 Nov. 2001   Bottling   Filling   Sanitation   01 ufc   —   01 ufc                   Valve   Water       31   23 Nov. 2001   Bottling   Filling   Sanitation   00 ufc   —   03 ufc                   Valve   Water       32   23 Nov. 2001   Bottling   Filling   Sanitation   01 ufc   —   01 ufc                   Valve   Water       33   24 Nov. 2001   Bottling   Filling   Sanitation   00 ufc   —   00 ufc                   Valve   Water       34   24 Nov. 2001   Bottling   Filling   Sanitation   00 ufc   —   00 ufc                   Valve   Water       35   24 Nov. 2001   Bottling   Filling   Sanitation   00 ufc       00 ufc                   Valve   Water       36   26 Nov. 2001   Quality   Laboratory   Prepared   TNTC   TNTC   TNTC               Control       37   26 Nov. 2001   Quality   Laboratory   Prepared   TNTC   TNTC   TNTC               Control       38   26 Nov. 2001   Quality   Laboratory   Prepared   TNTC   TNTC   TNTC               Control       39   26 Nov. 2001   Quality   Laboratory   Prepared   TNTC   TNTC   TNTC               Control       40   26 Nov. 2001   Quality   Laboratory   Prepared   TNTC   TNTC   TNTC               Control       41   28 Nov. 2001   Quality   Laboratory   Prepared   TNTC   TNTC   TNTC               Control       42   28 Nov. 2001   Quality   Laboratory   Prepared   TNTC   TNTC   TNTC               Control       43   28 Nov. 2001   Quality   Laboratory   Prepared   TNTC   TNTC   TNTC               Control       44   28 Nov. 2001   Quality   Laboratory   Prepared   TNTC   TNTC   TNTC               Control       45   28 Nov. 2001   Quality   Laboratory   Prepared   TNTC   TNTC   TNTC               Control       46   29 Nov. 2001   Bottling   Filling   Sanitation   00 ufc   00 ufc   00 ufc                   Valve   Water       47   29 Nov. 2001   Bottling   Filling   Sanitation   00 ufc   00 ufc   00 ufc                   Valve   Water       48   29 Nov. 2001   Bottling   Filling   Sanitation   00 ufc   00 ufc   00 ufc                   Valve   Water       49   29 Nov. 2001   Bottling   Filling   Sanitation   00 ufc   00 ufc   00 ufc                   Valve   Water       50   29 Nov. 2001   Bottling   Filling   Sanitation   00 ufc   00 ufc   00 ufc                   Valve   Water       51   28 Jan. 2002   Bottling   Filling   Sanitation   04 ufc   —   00 ufc                   Valve   Water       52   28 Jan. 2002   Bottling   Filling   Sanitation   08 ufc   —   01 ufc                   Valve   Water       53   28 Jan. 2002   Bottling   Filling   Sanitation   05 ufc   —   01 ufc                   Valve   Water       54   28 Jan. 2002   Bottling   Filling   Sanitation   07 ufc   —   00 ufc                   Valve   Water       55   4 Mar. 2002   Quality   Laboratory   Prepared   TNTC   —   TNTC               Control       56   4 Mar. 2002   Quality   Laboratory   Prepared   TNTC   —   TNTC               Control       57   4 Mar. 2002   Quality   Laboratory   Prepared   TNTC   —   TNTC               Control       58   4 Mar. 2002   Quality   Laboratory   Prepared   TNTC   —   TNTC               Control       59   4 Mar. 2002   Quality   Laboratory   Prepared   TNTC   —   TNTC               Control       60   4 Mar. 2002   Quality   Laboratory   Prepared   TNTC   —   TNTC               Control       61   4 Mar. 2002   Quality   Laboratory   Prepared   TNTC   —   TNTC               Control       62   4 Mar. 2002   Quality   Laboratory   Prepared   TNTC   —   TNTC               Control       63   4 Mar. 2002   Quality   Laboratory   Prepared   TNTC   —   TNTC               Control       64   4 Mar. 2002   Quality   Laboratory   Prepared   TNTC   —   TNTC               Control       65   8 Feb. 2002   Quality   Laboratory   Prepared   TNTC   TNTC   —               Control       66   8 Feb. 2002   Quality   Laboratory   Prepared   TNTC   TNTC   —               Control       67   8 Feb. 2002   Quality   Laboratory   Prepared   TNTC   TNTC   —               Control       68   8 Feb. 2002   Quality   Laboratory   Prepared   TNTC   TNTC   —               Control       69   8 Feb. 2002   Quality   Laboratory   Prepared   TNTC   TNTC   —               Control       70   8 Feb. 2002   Quality   Laboratory   Prepared   TNTC   TNTC   —               Control       71   8 Feb. 2002   Quality   Laboratory   Prepared   TNTC   TNTC   —               Control       72   8 Feb. 2002   Quality   Laboratory   Prepared   TNTC   TNTC   —               Control       73   8 Feb. 2002   Quality   Laboratory   Prepared   TNTC   TNTC   —               Control       74   8 Feb. 2002   Quality   Laboratory   Prepared   TNTC   TNTC   —               Control       75   19 Mar. 2001   Quality   Laboratory   Common   19 ufc   17 ufc   —               Control       Water       76   19 Mar. 2001   Quality   Laboratory   Common   26 ufc   23 ufc   —               Control       Water       77   19 Mar. 2001   Quality   Laboratory   Common   17 ufc   20 ufc   —               Control       Water       78   19 Mar. 2001   Quality   Laboratory   Common   23 ufc   14 ufc   —               Control       Water       79   19 Mar. 2001   Quality   Laboratory   Common   20 ufc   16 ufc   —               Control       Water       80   19 Mar. 2001   Quality   Laboratory   Common   24 ufc   25 ufc   —               Control       Water       81   26 Mar. 2002   Water Sector   Tank 1050   Raw   01 ufc   01 ufc   —                       Water       82   26 Mar. 2002   Quality   Laboratory   Water   00 ufc   00 ufc   —               Control       83   26 Mar. 2002   Quality   Laboratory   Common   01 ufc   02 ufc   —               Control       Water       84   26 Mar. 2002   Water Room   Outlet FP1   Treated   02 ufc   01 ufc   —                       Water       85   26 Mar. 2002   Water Room   Tank 600   Chlorinated   00 ufc   00 ufc   —                       Water       86   27 Mar. 2002   Water Room   Outlet FP1   Treated   00 ufc   00 ufc   —                       Water       87   27 Mar. 2002   Bottling   Filling   Sanitation   02 ufc   01 ufc   —                   Valve   Water       88   27 Mar. 2002   Bottling   Filling   Sanitation   01 ufc   01 ufc   —                   Valve   Water       89   27 Mar. 2002   Bottling   Filling   Sanitation   00 ufc   00 ufc   —                   Valve   Water       90   27 Mar. 2002   Bottling   Filling   Sanitation   00 ufc   00 ufc   —                   Valve   Water       91   27 Mar. 2002   Bottling   Filling   Sanitation   05 ufc   03 ufc   —                   Valve   Water       92   27 Mar. 2002   Bottling   Filling   Sanitation   03 ufc   04 ufc   —                   Valve   Water       93   27 Mar. 2002   Bottling   Filling   Sanitation   00 ufc   00 ufc   —                   Valve   Water       94   27 Mar. 2002   Bottling   Filling   Sanitation   00 ufc   00 ufc   —                   Valve   Water       95   27 Mar. 2002   Bottling   Filling   Sanitation   00 ufc   00 ufc   —                   Valve   Water       96   27 Mar. 2002   Bottling   Filling   Sanitation   00 ufc   00 ufc   —                   Valve   Water       97   27 Mar. 2002   Bottling   Filling   Sanitation   00 ufc   01 ufc   —                   Valve   Water       98   27 Mar. 2002   Bottling   Filling   Sanitation   01 ufc   01 ufc   —                   Valve   Water       99   27 Mar. 2002   Bottling   Filling   Sanitation   00 ufc   00 ufc   —                   Valve   Water       100   27 Mar. 2002   Bottling   Filling   Sanitation   03 ufc   01 ufc   —                   Valve   Water       101   27 Mar. 2002   Bottling   Filling   Sanitation   05 ufc   02 ufc   —                   Valve   Water       102   27 Mar. 2002   Bottling   Filling   Sanitation   02 ufc   01 ufc   —                   Valve   Water       103   27 Mar. 2002   Bottling   Filling   Sanitation   02 ufc   03 ufc   —                   Valve   Water       104   27 Mar. 2002   Bottling   Filling   Sanitation   00 ufc   00 ufc   —                   Valve   Water       105   27 Mar. 2002   Bottling   Filling   Sanitation   01 ufc   00 ufc   —                   Valve   Water       106   27 Mar. 2002   Bottling   Filling   Sanitation   00 ufc   00 ufc   —                   Valve   Water       107   27 Mar. 2002   Bottling   Filling   Sanitation   00 ufc   00 ufc   —                   Valve   Water       108   27 Mar. 2002   Bottling   Filling   Sanitation   02 ufc   00 ufc   —                   Valve   Water       109   27 Mar. 2002   Bottling   Filling   Sanitation   03 ufc   02 ufc   —                   Valve   Water       110   27 Mar. 2002   Bottling   Filling   Sanitation   00 ufc   01 ufc   —                   Valve   Water       111   27 Mar. 2002   Bottling   Filling   Sanitation   00 ufc   01 ufc   —                   Valve   Water       112   27 Mar. 2002   Bottling   Filling   Sanitation   00 ufc   00 ufc   —                   Valve   Water       113   27 Mar. 2002   Bottling   Filling   Sanitation   00 ufc   00 ufc   —                   Valve   Water       114   27 Mar. 2002   Bottling   Filling   Sanitation   01 ufc   00 ufc   —                   Valve   Water       115   27 Mar. 2002   Bottling   Filling   Sanitation   00 ufc   00 ufc   —                   Valve   Water       116   27 Mar. 2002   Bottling   Filling   Sanitation   00 ufc   00 ufc   —                   Valve   Water       117   27 Mar. 2002   Bottling   Filling   Sanitation   02 ufc   02 ufc   —                   Valve   Water       118   27 Mar. 2002   Bottling   Filling   Sanitation   01 ufc   00 ufc   —                   Valve   Water       119   27 Mar. 2002   Bottling   Tank JT   Sanitation   00 ufc   02 ufc   —                       Water       120   27 Mar. 2002   Bottling   Tank JT   Sanitation   07 ufc   05 ufc   —                       Water       121   27 Mar. 2002   Bottling   Tank JT   Sanitation   02 ufc   03 ufc   —                       Water       122   27 Mar. 2002   Bottling   Tank JT   Sanitation   00 ufc   00 ufc   —                       Water       123   27 Mar. 2002   Bottling   Tank JT   Sanitation   02 ufc   01 ufc   —                       Water       124   27 Mar. 2002   Bottling   Tank JT   Sanitation   02 ufc   01 ufc   —                       Water       125   27 Mar. 2002   Bottling   Tank JT   Sanitation   00 ufc   01 ufc   —                       Water       126   27 Mar. 2002   Bottling   Tank JT   Sanitation   03 ufc   01 ufc   —                       Water       127   27 Mar. 2002   Bottling   Beverage   Sanitation   03 ufc   00 ufc   —                   Tank   Water       128   27 Mar. 2002   Bottling   Beverage   Sanitation   02 ufc   01 ufc   —                   Tank   Water       129   27 Mar. 2002   Bottling   Beverage   Sanitation   00 ufc   00 ufc   —                   Tank   Water       130   27 Mar. 2002   Bottling   Beverage   Sanitation   01 ufc   00 ufc   —                   Tank   Water       131   27 Mar. 2002   Bottling   Beverage   Sanitation   02 ufc   04 ufc   —                   Tank   Water       132   27 Mar. 2002   Bottling   Beverage   Sanitation   01 ufc   02 ufc   —                   Tank   Water       133   27 Mar. 2002   Bottling   Beverage   Sanitation   00 ufc   00 ufc   —                   Tank   Water       134   27 Mar. 2002   Bottling   Beverage   Sanitation   02 ufc   00 ufc   —                   Tank   Water       135   27 Mar. 2002   Bottling   Final   Schweppes   00 ufc   00 ufc   —                   Product   Tonic       136   27 Mar. 2002   Bottling   Final   Schweppes   00 ufc   01 ufc   —                   Product   Tonic       137   27 Mar. 2002   Bottling   Final   Schweppes   00 ufc   00 ufc   —                   Product   Tonic       138   27 Mar. 2002   Bottling   Final   Schweppes   01 ufc   00 ufc   —                   Product   Tonic       139   27 Mar. 2002   Bottling   Final   Schweppes   00 ufc   00 ufc   —                   Product   Tonic       140   27 Mar. 2002   Bottling   Final   Schweppes   00 ufc   00 ufc   —                   Product   Tonic       141   27 Mar. 2002   Bottling   Final   Schweppes   02 ufc   00 ufc   —                   Product   Citrus       142   27 Mar. 2002   Bottling   Final   Schweppes   00 ufc   00 ufc   —                   Product   Citrus       143   27 Mar. 2002   Bottling   Final   Schweppes   00 ufc   00 ufc   —                   Product   Citrus       144   27 Mar. 2002   Bottling   Final   Schweppes   00 ufc   00 ufc   —                   Product   Citrus       145   27 Mar. 2002   Bottling   Final   Schweppes   00 ufc   01 ufc   —                   Product   Citrus       146   27 Mar. 2002   Bottling   Final   Schweppes   00 ufc   01 ufc   —                   Product   Citrus       147   27 Mar. 2002   Bottling   Final   Schweppes   00 ufc   00 ufc   —                   Product   Ginger Ale       148   27 Mar. 2002   Bottling   Final   Schweppes   00 ufc   00 ufc   —                   Product   Ginger Ale       149   27 Mar. 2002   Bottling   Final   Schweppes   00 ufc   00 ufc   —                   Product   Ginger Ale       150   27 Mar. 2002   Bottling   Final   Schweppes   00 ufc   02 ufc   —                   Product   Ginger Ale       151   27 Mar. 2002   Bottling   Final   Schweppes   00 ufc   00 ufc   —                   Product   Ginger Ale       152   27 Mar. 2002   Bottling   Final   Schweppes   00 ufc   00 ufc   —                   Product   Ginger Ale       153   27 Mar. 2002   Bottling   Final   Tai Pink   00 ufc   01 ufc   —                   Product   Grapefruit       154   27 Mar. 2002   Bottling   Final   Tai Pink   00 ufc   00 ufc   —                   Product   Grapefruit       155   27 Mar. 2002   Bottling   Final   Tai Pink   00 ufc   00 ufc   —                   Product   Grapefruit       156   27 Mar. 2002   Bottling   Final   Tai Pink   00 ufc   00 ufc   —                   Product   Grapefruit       157   27 Mar. 2002   Bottling   Final   Tai Pink   00 ufc   00 ufc   —                   Product   Grapefruit       158   27 Mar. 2002   Bottling   Final   Tai Pink   00 ufc   00 ufc   —                   Product   Grapefruit       159   27 Mar. 2002   Syrup   Contimol   Sanitation   00 ufc   00 ufc   —               Room       Water       160   27 Mar. 2002   Syrup   Pasteurizer   Sanitation   01 ufc   00 ufc   —               Room       Water       161   27 Mar. 2002   Syrup   Filter TLG   Sanitation   00 ufc   00 ufc   —               Room       Water       162   27 Mar. 2002   Syrup   Tank Buffer   Sanitation   03 ufc   02 ufc   —               Room       Water       163   27 Mar. 2002   Syrup   Gravimol 1   Sanitation   02 ufc   01 ufc   —               Room       Water       164   27 Mar. 2002   Syrup   Gravimol 1   Sanitation   05 ufc   02 ufc   —               Room       Water       165   27 Mar. 2002   Syrup   Gravimol 1   Sanitation   06 ufc   05 ufc   —               Room       Water       166   27 Mar. 2002   Syrup   Gravimol 1   Sanitation   09 ufc   06 ufc   —               Room       Water       167   27 Mar. 2002   Syrup   Gravimol 1   Sanitation   01 ufc   00 ufc   —               Room       Water       168   27 Mar. 2002   Syrup   Tank JS 1   Sanitation   00 ufc   00 ufc   —               Room       Water       169   27 Mar. 2002   Syrup   Tank JT 10   Sanitation   00 ufc   00 ufc   —               Room       Water       170   27 Mar. 2002   Syrup   Tank JT 5   Sanitation   01 ufc   00 ufc   —               Room       Water       171   27 Mar. 2002   Syrup   Tank JT 4   Sanitation   02 ufc   00 ufc   —               Room       Water       172   27 Mar. 2002   Syrup   Tank JT 2   Sanitation   04 ufc   05 ufc   —               Room       Water       173   27 Mar. 2002   Syrup   Tank JT 1   Sanitation   00 ufc   02 ufc   —               Room       Water       174   27 Mar. 2002   Syrup   Tank JT 6   Sanitation   03 ufc   00 ufc   —               Room       Water       175   27 Mar. 2002   Syrup   Tank JT 1   Sanitation   06 ufc   03 ufc   —               Room       Water       176   27 Mar. 2002   Syrup   Tank JT 5   Sanitation   06 ufc   02 ufc   —               Room       Water       177   27 Mar. 2002   Syrup   Dispenser   Sanitation   02 ufc   01 ufc   —               Room   Line   Water       178   27 Mar. 2002   Unloading   Transport   Fructose   00 ufc   01 ufc   —       179   27 Mar. 2002   Unloading   Transport   Fructose   02 ufc   04 ufc   —       180   27 Mar. 2002   Unloading   Transport   Fructose   03 ufc   01 ufc   —       181   27 Mar. 2002   Unloading   Transport   Fructose   00 ufc   01 ufc   —       182   27 Mar. 2002   Unloading   Transport   Fructose   01 ufc   02 ufc   —       183   27 Mar. 2002   Unloading   Transport   Fructose   00 ufc   00 ufc   —       184   27 Mar. 2002   Syrup   Tank JT   Coca   00 ufc   01 ufc   —               Room       Cola       185   27 Mar. 2002   Syrup   Tank JT   Coca   01 ufc   00 ufc   —               Room       Cola                       Light       186   27 Mar. 2002   Syrup   Tank JT   Schweppes   00 ufc   00 ufc   —               Room       Citrus       187   27 Mar. 2002   Syrup   Tank JT   Coca   02 ufc   00 ufc   —               Room       Cola       188   27 Mar. 2002   Syrup   Tank JT   Coca   00 ufc   01 ufc   —               Room       Cola       189   27 Mar. 2002   Syrup   Tank JT   Coca   02 ufc   01 ufc   —               Room       Cola                       Light       190   27 Mar. 2002   Syrup   Tank JT   Schweppes   01 ufc   01 ufc   —               Room       Ginger Ale       191   27 Mar. 2002   Syrup   Tank JT   Coca   03 ufc   00 ufc   —               Room       Cola                       Light       192   27 Mar. 2002   Syrup   Tank JT   Coca   00 ufc   02 ufc   —               Room       Cola       193   27 Mar. 2002   Syrup   Tank JT   Tai Pink   00 ufc   00 ufc   —               Room       Grapefruit       194   27 Mar. 2002   Syrup   Tank JT   Coca   00 ufc   00 ufc   —               Room       Cola                       Light       195   27 Mar. 2002   Syrup   Tank JT   Coca   00 ufc   01 ufc   —               Room       Cola       196   27 Mar. 2002   Syrup   Tank JT   Coca   01 ufc   00 ufc   —               Room       Cola       197   28 Mar. 2002   Unloading   Transport   Fructose   00 ufc   01 ufc   —       198   28 Mar. 2002   Unloading   Transport   Fructose   01 ufc   00 ufc   —       199   28 Mar. 2002   Unloading   Transport   Fructose   04 ufc   01 ufc   —       200   28 Mar. 2002   Unloading   Transport   Fructose   02 ufc   00 ufc   —       201   12 Apr. 2002   Water   Outlet PC1   Treated   00 ufc   02 ufc   —               Room       Water       202   12 Apr. 2002   Water   Outlet PC2   Treated   03 ufc   04 ufc   —               Room       Water       203   12 Apr. 2002   Water   Outlet PC3   Treated   00 ufc   04 ufc   —               Room       Water       204   12 Apr. 2002   Water   Outlet PC4   Treated   00 ufc   01 ufc   —               Room       Water       205   12 Apr. 2002   Water   Outlet FP1   Treated   00 ufc   00 ufc   —               Room       Water       206   12 Apr. 2002   Water   Outlet FP2   Treated   00 ufc   00 ufc   —               Room       Water       207   12 Apr. 2002   Water   Outlet FP3   Treated   02 ufc   00 ufc   —               Room       Water       208   12 Apr. 2002   Water   Outlet FP4   Treated   00 ufc   00 ufc   —               Room       Water       209   12 Apr. 2002   Water   Tank 600   Treated   00 ufc   00 ufc   —               Room       Water       210   12 Apr. 2002   Water   Tank 1050   Raw   00 ufc   00 ufc   —               Room       Water       211   12 Apr. 2002   Water   Inlet FP1   Treated   00 ufc   02 ufc   —               Room       Water       212   12 Apr. 2002   Water   Inlet FP2   Treated   00 ufc   01 ufc   —               Room       Water       213   16 Apr. 2002   Quality   Laboratory   Prepared   36 ufc   —   44 ufc               Control       214   16 Apr. 2002   Quality   Laboratory   Prepared   42 ufc   —   41 ufc               Control       215   16 Apr. 2002   Quality   Laboratory   Prepared   00 ufc   —   00 ufc               Control       216   16 Apr. 2002   Quality   Laboratory   Prepared   01 ufc   —   00 ufc               Control       217   16 Apr. 2002   Quality   Laboratory   Prepared   00 ufc   —   03 ufc               Control       218   16 Apr. 2002   Quality   Laboratory   Prepared   00 ufc   —   00 ufc               Control       219   16 Apr. 2002   Quality   Laboratory   Prepared   00 ufc   —   00 ufc               Control       220   16 Apr. 2002   Quality   Laboratory   Prepared   00 ufc   —   00 ufc               Control       221   16 Apr. 2002   Quality   Laboratory   Prepared   01 ufc   —   01 ufc               Control       222   16 Apr. 2002   Quality   Laboratory   Prepared   00 ufc   —   00 ufc               Control       223   16 Apr. 2002   Quality   Laboratory   Prepared   00 ufc   —   00 ufc               Control       224   16 Apr. 2002   Quality   Laboratory   Prepared   00 ufc   —   00 ufc               Control       225   23 Apr. 2002   Quality   Laboratory   Prepared   63 ufc   58 ufc   —               Control       226   23 Apr. 2002   Quality   Laboratory   Prepared   52 ufc   55 ufc   —               Control       227   23 Apr. 2002   Quality   Laboratory   Prepared   36 ufc   30 ufc   —               Control       228   23 Apr. 2002   Quality   Laboratory   Prepared   15 ufc   09 ufc   —               Control       229   23 Apr. 2002   Quality   Laboratory   Prepared   02 ufc   00 ufc   —               Control       230   23 Apr. 2002   Quality   Laboratory   Prepared   08 ufc   04 ufc   —               Control       231   23 Apr. 2002   Quality   Laboratory   Prepared   22 ufc   17 ufc   —               Control       232   23 Apr. 2002   Quality   Laboratory   Prepared   00 ufc   00 ufc   —               Control       233   23 Apr. 2002   Quality   Laboratory   Prepared   00 ufc   00 ufc   —               Control       234   23 Apr. 2002   Quality   Laboratory   Prepared   00 ufc   00 ufc   —               Control       235   23 Apr. 2002   Quality   Laboratory   Prepared   12 ufc   10 ufc   —               Control       236   23 Apr. 2002   Quality   Laboratory   Prepared   126 ufc   131 ufc   —               Control       237   23 Apr. 2002   Quality   Laboratory   Prepared   98 ufc   106 ufc   —               Control       238   23 Apr. 2002   Quality   Laboratory   Prepared   82 ufc   80 ufc   —               Control       239   23 Apr. 2002   Quality   Laboratory   Prepared   41 ufc   35 ufc   —               Control       240   23 Apr. 2002   Quality   Laboratory   Prepared   37 ufc   42 ufc   —               Control       241   23 Apr. 2002   Quality   Laboratory   Prepared   30 ufc   35 ufc   —               Control       242   23 Apr. 2002   Quality   Laboratory   Prepared   22 ufc   16 ufc   —               Control       243   23 Apr. 2002   Quality   Laboratory   Prepared   27 ufc   20 ufc   —               Control       244   23 Apr. 2002   Quality   Laboratory   Prepared   29 ufc   21 ufc   —               Control       245   23 Apr. 2002   Quality   Laboratory   Prepared   23 ufc   27 ufc   —               Control       246   23 Apr. 2002   Quality   Laboratory   Prepared   00 ufc   00 ufc   —               Control       247   23 Apr. 2002   Quality   Laboratory   Prepared   00 ufc   00 ufc   —               Control       248   23 Apr. 2002   Quality   Laboratory   Prepared   02 ufc   00 ufc   —               Control       249   23 Apr. 2002   Quality   Laboratory   Prepared   01 ufc   00 ufc   —               Control       250   23 Apr. 2002   Quality   Laboratory   Prepared   00 ufc   00 ufc   —               Control       251   3 May 2002   Syrup   Gravimol 1   Sanitation   03 ufc   01 ufc   —               Room       Water       252   3 May 2002   Syrup   Gravimol 1   Sanitation   00 ufc   01 ufc   —               Room       Water       253   3 May 2002   Syrup   Gravimol 1   Sanitation   02 ufc   01 ufc   —               Room       Water       254   3 May 2002   Syrup   Gravimol 2   Sanitation   02 ufc   03 ufc   —               Room       Water       255   3 May 2002   Syrup   Gravimol 2   Sanitation   00 ufc   00 ufc   —               Room       Water       256   3 May 2002   Syrup   Gravimol 2   Sanitation   02 ufc   02 ufc   —               Room       Water       257   3 May 2002   Syrup   Gravimol 2   Sanitation   04 ufc   02 ufc   —               Room       Water       258   3 May 2002   Syrup   Gravimol 2   Sanitation   04 ufc   04 ufc   —               Room       Water       259   3 May 2002   Syrup   Tank JT 6   Sanitation   01 ufc   01 ufc   —               Room       Water       260   3 May 2002   Syrup   Tank JT 1   Sanitation   03 ufc   00 ufc   —               Room       Water       261   3 May 2002   Syrup   Tank JT 7   Sanitation   01 ufc   03 ufc   —               Room       Water       262   3 May 2002   Syrup   Tank JT 8   Sanitation   00 ufc   01 ufc   —               Room       Water       263   3 May 2002   Syrup   Tank JT 11   Sanitation   00 ufc   01 ufc   —               Room       Water       264   3 May 2002   Syrup   Tank JT 3   Sanitation   01 ufc   02 ufc   —               Room       Water       265   3 May 2002   Syrup   Tank JT 2   Sanitation   04 ufc   01 ufc   —               Room       Water       266   3 May 2002   Syrup   Tank JT 7   Sanitation   02 ufc   02 ufc   —               Room       Water       267   3 May 2002   Unloading   Transport   Fructose   00 ufc   00 ufc   —       268   3 May 2002   Unloading   Transport   Fructose   02 ufc   01 ufc   —       269   3 May 2002   Unloading   Transport   Fructose   02 ufc   00 ufc   —       270   3 May 2002   Unloading   Transport   Fructose   00 ufc   00 ufc   —       271   3 May 2002   Unloading   Transport   Fructose   00 ufc   00 ufc   —       272   3 May 2002   Unloading   Transport   Fructose   01 ufc   02 ufc   —       273   3 May 2002   Unloading   Transport   Fructose   01 ufc   00 ufc   —       274   3 May 2002   Unloading   Transport   Fructose   00 ufc   00 ufc   —       275   3 May 2002   Unloading   Transport   Fructose   01 ufc   02 ufc   —       276   3 May 2002   Unloading   Transport   Fructose   05 ufc   02 ufc   —       277   3 May 2002   Unloading   Transport   Fructose   04 ufc   02 ufc   —       278   3 May 2002   Unloading   Transport   Fructose   00 ufc   01 ufc   —       279   3 May 2002   Syrup   Tank JT   Fanta   00 ufc   00 ufc   —               Room       Orange       280   3 May 2002   Syrup   Tank JT   Tai Lime   00 ufc   00 ufc   —               Room       Lemon       281   3 May 2002   Syrup   Tank JT   Fanta   00 ufc   01 ufc   —               Room       Tonic       282   3 May 2002   Syrup   Tank JT   Coca   01 ufc   00 ufc   —               Room       Cola                       Light       283   3 May 2002   Syrup   Tank JT   Sprite   00 ufc   00 ufc   —               Room       Light       284   3 May 2002   Syrup   Tank JT   Sprite   01 ufc   00 ufc   —               Room       285   3 May 2002   Syrup   Tank JT   Coca   00 ufc   00 ufc   —               Room       Cola                       Light       286   3 May 2002   Syrup   Tank JT   Coca   02 ufc   03 ufc   —               Room       Cola       287   3 May 2002   Syrup   Tank JT   Fanta   00 ufc   00 ufc   —               Room       Orange       288   3 May 2002   Syrup   Tank JT   Sprite   00 ufc   00 ufc   —               Room       289   3 May 2002   Syrup   Tank JT   Coca   03 ufc   02 ufc   —               Room       Cola       290   3 May 2002   Syrup   Tank JT   Coca   00 ufc   00 ufc   —               Room       Cola                       Light       291   3 May 2002   Syrup   Tank JT   Quatro   03 ufc   03 ufc   —               Room       Grapefruit       292   7 May 2002   Quality   Laboratory   Prepared   00 ufc   00 ufc   —               Control       293   7 May 2002   Quality   Laboratory   Prepared   00 ufc   00 ufc   —               Control       294   7 May 2002   Quality   Laboratory   Prepared   01 ufc   00 ufc   —               Control       295   7 May 2002   Quality   Laboratory   Prepared   04 ufc   02 ufc   —               Control       296   7 May 2002   Quality   Laboratory   Prepared   00 ufc   00 ufc   —               Control       297   7 May 2002   Quality   Laboratory   Prepared   04 ufc   04 ufc   —               Control       298   7 May 2002   Quality   Laboratory   Prepared   00 ufc   01 ufc   —               Control       299   7 May 2002   Quality   Laboratory   Prepared   00 ufc   00 ufc   —               Control       300   7 May 2002   Quality   Laboratory   Prepared   00 ufc   00 ufc   —               Control       301   16 May 2002   Quality   Laboratory   Prepared   05 ufc   03 ufc   —               Control       302   16 May 2002   Quality   Laboratory   Prepared   03 ufc   00 ufc   —               Control       303   16 May 2002   Quality   Laboratory   Prepared   09 ufc   06 ufc   —               Control       304   16 May 2002   Quality   Laboratory   Prepared   07 ufc   05 ufc   —               Control       305   16 May 2002   Quality   Laboratory   Prepared   05 ufc   08 ufc   —               Control       306   16 May 2002   Quality   Laboratory   Prepared   06 ufc   03 ufc   —               Control       307   16 May 2002   Quality   Laboratory   Prepared   02 ufc   00 ufc   —               Control       308   16 May 2002   Quality   Laboratory   Prepared   04 ufc   01 ufc   —               Control       309   16 May 2002   Quality   Laboratory   Prepared   09 ufc   07 ufc   —               Control       310   16 May 2002   Quality   Laboratory   Prepared   07 ufc   02 ufc   —               Control       311   16 May 2002   Quality   Laboratory   Prepared   86 ufc   79 ufc   —               Control       312   16 May 2002   Quality   Laboratory   Prepared   112 ufc   104 ufc   —               Control       313   16 May 2002   Quality   Laboratory   Prepared   93 ufc   108 ufc   —               Control       314   16 May 2002   Quality   Laboratory   Prepared   90 ufc   99 ufc   —               Control       315   16 May 2002   Quality   Laboratory   Prepared   78 ufc   81 ufc   —               Control       316   16 May 2002   Quality   Laboratory   Prepared   101 ufc   89 ufc   —               Control       317   16 May 2002   Quality   Laboratory   Prepared   91 ufc   82 ufc   —               Control       318   16 May 2002   Quality   Laboratory   Prepared   88 ufc   90 ufc   —               Control       319   16 May 2002   Quality   Laboratory   Prepared   94 ufc   90 ufc   —               Control       320   16 May 2002   Quality   Laboratory   Prepared   96 ufc   84 ufc   —               Control       321   29 May 2001   Bottling   Filling   Sanitation   08 ufc   05 ufc   —                   Valve   Water       322   29 May 2001   Bottling   Filling   Sanitation   10 ufc   07 ufc   —                   Valve   Water       323   29 May 2001   Bottling   Filling   Sanitation   06 ufc   05 ufc   —                   Valve   Water       324   29 May 2001   Bottling   Filling   Sanitation   05 ufc   08 ufc   —                   Valve   Water       325   29 May 2001   Bottling   Filling   Sanitation   06 ufc   03 ufc   —                   Valve   Water       326   29 May 2001   Bottling   Filling   Sanitation   05 ufc   07 ufc   —                   Valve   Water       327   29 May 2001   Bottling   Filling   Sanitation   07 ufc   07 ufc   —                   Valve   Water       328   29 May 2001   Bottling   Filling   Sanitation   09 ufc   06 ufc   —                   Valve   Water       329   29 May 2001   Bottling   Filling   Sanitation   02 ufc   00 ufc   —                   Valve   Water       330   29 May 2001   Bottling   Filling   Sanitation   04 ufc   06 ufc   —                   Valve   Water       331   29 May 2001   Bottling   Filling   Sanitation   05 ufc   02 ufc   —                   Valve   Water       332   29 May 2001   Bottling   Filling   Sanitation   08 ufc   08 ufc   —                   Valve   Water       333   14 Jun. 2001   Quality   Laboratory   Prepared   00 ufc   00 ufc   00 ufc               Control       334   14 Jun. 2001   Quality   Laboratory   Prepared   15 ufc   09 ufc   09 ufc               Control       335   14 Jun. 2001   Quality   Laboratory   Prepared   15 ufc   18 ufc   16 ufc               Control       336   14 Jun. 2001   Quality   Laboratory   Prepared   01 ufc   01 ufc   00 ufc               Control       337   14 Jun. 2001   Quality   Laboratory   Prepared   00 ufc   00 ufc   00 ufc               Control       338   14 Jun. 2001   Quality   Laboratory   Prepared   00 ufc   00 ufc   00 ufc               Control       339   3 Jul. 2001   Quality   Laboratory   Prepared   TNTC   TNTC   —               Control       340   3 Jul. 2001   Quality   Laboratory   Prepared   TNTC   TNTC   —               Control       341   3 Jul. 2001   Quality   Laboratory   Prepared   TNTC   TNTC   —               Control       342   3 Jul. 2001   Quality   Laboratory   Prepared   01 ufc   00 ufc   —               Control       343   3 Jul. 2001   Quality   Laboratory   Prepared   00 ufc   00 ufc   —               Control       344   3 Jul. 2001   Quality   Laboratory   Prepared   TNTC   TNTC   —               Control       345   24 Jul. 2002   Line 2   Filling   Sanitation   01 ufc   00 ufc   —                   Valve   Water       346   24 Jul. 2002   Line 2   Filling   Sanitation   08 ufc   05 ufc   —                   Valve   Water       347   24 Jul. 2002   Line 2   Filling   Sanitation   03 ufc   02 ufc   —                   Valve   Water       348   24 Jul. 2002   Line 2   Filling   Sanitation   00 ufc   01 ufc   —                   Valve   Water       349   25 Jul. 2002   Line 2   Filling   Sanitation   02 ufc   03 ufc   —                   Valve   Water       350   25 Jul. 2002   Line 2   Filling   Sanitation   02 ufc   04 ufc   —                   Valve   Water       351   25 Jul. 2002   Line 2   Filling   Sanitation   04 ufc   01 ufc   —                   Valve   Water       52   25 Jul. 2002   Line 2   Filling   Sanitation   02 ufc   03 ufc   —                   Valve   Water       353   25 Jul. 2002   Line 2   Filling   Sanitation   06 ufc   08 ufc   —                   Valve   Water       354   25 Jul. 2002   Line 2   Filling   Sanitation   07 ufc   10 ufc   —                   Valve   Water       355   25 Jul. 2002   Line 2   Filling   Sanitation   09 ufc   07 ufc   —                   Valve   Water       356   25 Jul. 2002   Line 2   Filling   Sanitation   05 ufc   07 ufc   —                   Valve   Water       357   25 Jul. 2002   Line 2   Filling   Sanitation   10 ufc   08 ufc   —                   Valve   Water       358   25 Jul. 2002   Line 2   Filling   Sanitation   06 ufc   08 ufc   —                   Valve   Water       359   25 Jul. 2002   Line 2   Filling   Sanitation   04 ufc   07 ufc   —                   Valve   Water       360   25 Jul. 2002   Line 2   Filling   Sanitation   08 ufc   08 ufc   —                   Valve   Water       361   26 Jul. 2002   Line 2   Filling   Sanitation   04 ufc   06 ufc   —                   Valve   Water       362   26 Jul. 2002   Line 2   Filling   Sanitation   08 ufc   05 ufc   —                   Valve   Water       363   26 Jul. 2002   Line 2   Filling   Sanitation   10 ufc   07 ufc   —                   Valve   Water       364   26 Jul. 2002   Line 2   Filling   Sanitation   09 ufc   07 ufc   —                   Valve   Water       365   27 Jul. 2002   Line 2   Filling   Sanitation   05 ufc   06 ufc   —                   Valve   Water       366   27 Jul. 2002   Line 2   Filling   Sanitation   07 ufc   06 ufc   —                   Valve   Water       367   27 Jul. 2002   Line 2   Filling   Sanitation   05 ufc   02 ufc   —                   Valve   Water       368   27 Jul. 2002   Line 2   Filling   Sanitation   05 ufc   06 ufc   —                   Valve   Water       369   29 Jul. 2002   Line 2   Filling   Sanitation   04 ufc   02 ufc   —                   Valve   Water       370   29 Jul. 2002   Line 2   Filling   Sanitation   02 ufc   03 ufc   —                   Valve   Water       371   29 Jul. 2002   Line 2   Filling   Sanitation   01 ufc   00 ufc   —                   Valve   Water       372   29 Jul. 2002   Line 2   Filling   Sanitation   03 ufc   05 ufc   —                   Valve   Water       373   29 Jul. 2002   Line 2   Filling   Sanitation   04 ufc   08 ufc   —                   Valve   Water       374   29 Jul. 2002   Line 2   Filling   Sanitation   10 ufc   09 ufc   —                   Valve   Water       375   29 Jul. 2002   Line 2   Filling   Sanitation   06 ufc   07 ufc   —                   Valve   Water       376   29 Jul. 2002   Line 2   Filling   Sanitation   06 ufc   08 ufc   —                   Valve   Water       377   30 Jul. 2002   Line 2   Filling   Sanitation   07 ufc   09 ufc   —                   Valve   Water       378   30 Jul. 2002   Line 2   Filling   Sanitation   10 ufc   08 ufc   —                   Valve   Water       379   30 Jul. 2002   Line 2   Filling   Sanitation   09 ufc   06 ufc   —                   Valve   Water       380   30 Jul. 2002   Line 2   Filling   Sanitation   07 ufc   08 ufc   —                   Valve   Water       381   30 Jul. 2002   Line 2   Filling   Sanitation   05 ufc   09 ufc   —                   Valve   Water       382   30 Jul. 2002   Line 2   Filling   Sanitation   08 ufc   09 ufc   —                   Valve   Water       383   30 Jul. 2002   Line 2   Filling   Sanitation   08 ufc   08 ufc   —                   Valve   Water       384   30 Jul. 2002   Line 2   Filling   Sanitation   09 ufc   06 ufc   —                   Valve   Water       385   30 Jul. 2002   Line 2   Filling   Sanitation   09 ufc   08 ufc   —                   Valve   Water       386   30 Jul. 2002   Line 2   Filling   Sanitation   06 ufc   08 ufc   —                   Valve   Water       387   30 Jul. 2002   Line 2   Filling   Sanitation   08 ufc   06 ufc   —                   Valve   Water       388   30 Jul. 2002   Line 2   Filling   Sanitation   07 ufc   05 ufc   —                   Valve   Water       389   31 Jul. 2002   Line 2   Filling   Sanitation   09 ufc   09 ufc   —                   Valve   Water       390   31 Jul. 2002   Line 2   Filling   Sanitation   09 ufc   08 ufc   —                   Valve   Water       391   31 Jul. 2002   Line 2   Filling   Sanitation   04 ufc   07 ufc   —                   Valve   Water       392   31 Jul. 2002   Line 2   Filling   Sanitation   07 ufc   09 ufc   —                   Valve   Water       393   1 Aug. 2002   Line 2   Filling   Sanitation   05 ufc   03 ufc   —                   Valve   Water       394   1 Aug. 2002   Line 2   Filling   Sanitation   06 ufc   06 ufc   —                   Valve   Water       395   1 Aug. 2002   Line 2   Filling   Sanitation   06 ufc   05 ufc   —                   Valve   Water       396   1 Aug. 2002   Line 2   Filling   Sanitation   08 ufc   05 ufc   —                   Valve   Water       397   2 Aug. 2002   Line 2   Filling   Sanitation   09 ufc   07 ufc   —                   Valve   Water       398   2 Aug. 2002   Line 2   Filling   Sanitation   06 ufc   06 ufc   —                   Valve   Water       399   2 Aug. 2002   Line 2   Filling   Sanitation   06 ufc   08 ufc   —                   Valve   Water       400   2 Aug. 2002   Line 2   Filling   Sanitation   07 ufc   05 ufc   —                   Valve   Water       401   2 Aug. 2002   Line 2   Filling   Sanitation   02 ufc   04 ufc   —                   Valve   Water       402   2 Aug. 2002   Line 2   Filling   Sanitation   05 ufc   05 ufc   —                   Valve   Water       403   2 Aug. 2002   Line 2   Filling   Sanitation   05 ufc   02 ufc   —                   Valve   Water       404   2 Aug. 2002   Line 2   Filling   Sanitation   09 ufc   05 ufc   —                   Valve   Water       405   3 Aug. 2002   Line 2   Filling   Sanitation   06 ufc   03 ufc   —                   Valve   Water       406   3 Aug. 2002   Line 2   Filling   Sanitation   08 ufc   05 ufc   —                   Valve   Water       407   3 Aug. 2002   Line 2   Filling   Sanitation   00 ufc   02 ufc   —                   Valve   Water       408   3 Aug. 2002   Line 2   Filling   Sanitation   04 ufc   02 ufc   —                   Valve   Water       409   3 Aug. 2002   Line 2   Filling   Sanitation   03 ufc   07 ufc   —                   Valve   Water       410   3 Aug. 2002   Line 2   Filling   Sanitation   10 ufc   10 ufc   —                   Valve   Water       411   3 Aug. 2002   Line 2   Filling   Sanitation   07 ufc   09 ufc   —                   Valve   Water       412   3 Aug. 2002   Line 2   Filling   Sanitation   09 ufc   09 ufc   —                   Valve   Water       413   5 Aug. 2002   Line 2   Filling   Sanitation   06 ufc   05 ufc   —                   Valve   Water       414   5 Aug. 2002   Line 2   Filling   Sanitation   05 ufc   07 ufc   —                   Valve   Water       415   5 Aug. 2002   Line 2   Filling   Sanitation   03 ufc   06 ufc   —                   Valve   Water       416   5 Aug. 2002   Line 2   Filling   Sanitation   04 ufc   06 ufc   —                   Valve   Water       417   7 Aug. 2002   Line 2   Filling   Sanitation   05 ufc   02 ufc   —                   Valve   Water       418   7 Aug. 2002   Line 2   Filling   Sanitation   06 ufc   02 ufc   —                   Valve   Water       419   7 Aug. 2002   Line 2   Filling   Sanitation   08 ufc   06 ufc   —                   Valve   Water       420   7 Aug. 2002   Line 2   Filling   Sanitation   09 ufc   09 ufc   —                   Valve   Water       421   8 Aug. 2002   Line 2   Filling   Sanitation   02 ufc   05 ufc   —                   Valve   Water       422   8 Aug. 2002   Line 2   Filling   Sanitation   08 ufc   07 ufc   —                   Valve   Water       423   8 Aug. 2002   Line 2   Filling   Sanitation   07 ufc   02 ufc   —                   Valve   Water       424   8 Aug. 2002   Line 2   Filling   Sanitation   10 ufc   06 ufc   —                   Valve   Water       425   8 Aug. 2002   Line 2   Filling   Sanitation   09 ufc   04 ufc   —                   Valve   Water       426   8 Aug. 2002   Line 2   Filling   Sanitation   05 ufc   07 ufc   —                   Valve   Water       427   9 Aug. 2002   Line 2   Filling   Sanitation   07 ufc   07 ufc   —                   Valve   Water       428   9 Aug. 2002   Line 2   Filling   Sanitation   04 ufc   09 ufc   —                   Valve   Water       429   9 Aug. 2002   Line 2   Filling   Sanitation   05 ufc   03 ufc   —                   Valve   Water       430   9 Aug. 2002   Line 2   Filling   Sanitation   07 ufc   07 ufc   —                   Valve   Water       431   10 Aug. 2002   Line 2   Filling   Sanitation   06 ufc   07 ufc   —                   Valve   Water       432   10 Aug. 2002   Line 2   Filling   Sanitation   08 ufc   08 ufc   —                   Valve   Water       433   10 Aug. 2002   Line 2   Filling   Sanitation   09 ufc   08 ufc   —                   Valve   Water       434   10 Aug. 2002   Line 2   Filling   Sanitation   06 ufc   07 ufc   —                   Valve   Water       435   10 Aug. 2002   Line 2   Filling   Sanitation   04 ufc   04 ufc   —                   Valve   Water       436   10 Aug. 2002   Line 2   Filling   Sanitation   03 ufc   02 ufc   —                   Valve   Water       437   10 Aug. 2002   Line 2   Filling   Sanitation   00 ufc   00 ufc   —                   Valve   Water       438   10 Aug. 2002   Line 2   Filling   Sanitation   00 ufc   00 ufc   —                   Valve   Water       439   12 Aug. 2002   Line 2   Filling   Sanitation   05 ufc   05 ufc   —                   Valve   Water       440   12 Aug. 2002   Line 2   Filling   Sanitation   06 ufc   05 ufc   —                   Valve   Water       441   12 Aug. 2002   Line 2   Filling   Sanitation   02 ufc   03 ufc   —                   Valve   Water       442   12 Aug. 2002   Line 2   Filling   Sanitation   05 ufc   07 ufc   —                   Valve   Water       443   12 Aug. 2002   Line 2   Filling   Sanitation   04 ufc   07 ufc   —                   Valve   Water       444   12 Aug. 2002   Line 2   Filling   Sanitation   08 ufc   09 ufc   —                   Valve   Water       445   12 Aug. 2002   Line 2   Filling   Sanitation   10 ufc   08 ufc   —                   Valve   Water       446   12 Aug. 2002   Line 2   Filling   Sanitation   05 ufc   06 ufc   —                   Valve   Water       447   13 Aug. 2002   Line 2   Filling   Sanitation   05 ufc   05 ufc   —                   Valve   Water       448   13 Aug. 2002   Line 2   Filling   Sanitation   06 ufc   04 ufc   —                   Valve   Water       449   13 Aug. 2002   Line 2   Filling   Sanitation   08 ufc   04 ufc   —                   Valve   Water       450   13 Aug. 2002   Line 2   Filling   Sanitation   05 ufc   04 ufc   —                   Valve   Water       451   13 Aug. 2002   Line 2   Filling   Sanitation   05 ufc   04 ufc   —                   Valve   Water       452   13 Aug. 2002   Line 2   Filling   Sanitation   04 ufc   02 ufc   —                   Valve   Water       453   13 Aug. 2002   Line 2   Filling   Sanitation   01 ufc   00 ufc   —                   Valve   Water       454   13 Aug. 2002   Line 2   Filling   Sanitation   00 ufc   00 ufc   —                   Valve   Water       455   14 Aug. 2002   Line 2   Filling   Sanitation   00 ufc   00 ufc   —                   Valve   Water       456   14 Aug. 2002   Line 2   Filling   Sanitation   02 ufc   00 ufc   —                   Valve   Water       457   14 Aug. 2002   Line 2   Filling   Sanitation   02 ufc   02 ufc   —                   Valve   Water       458   14 Aug. 2002   Line 2   Filling   Sanitation   01 ufc   04 ufc   —                   Valve   Water       459   14 Aug. 2002   Line 2   Filling   Sanitation   00 ufc   02 ufc   —                   Valve   Water       460   14 Aug. 2002   Line 2   Filling   Sanitation   04 ufc   03 ufc   —                   Valve   Water       461   14 Aug. 2002   Line 2   Filling   Sanitation   01 ufc   00 ufc   —                   Valve   Water       462   14 Aug. 2002   Line 2   Filling   Sanitation   02 ufc   02 ufc   —                   Valve   Water       463   14 Aug. 2002   Line 2   Filling   Sanitation   02 ufc   01 ufc   —                   Valve   Water       464   14 Aug. 2002   Line 2   Filling   Sanitation   00 ufc   01 ufc   —                   Valve   Water       465   14 Aug. 2002   Line 2   Filling   Sanitation   00 ufc   00 ufc   —                   Valve   Water       466   14 Aug. 2002   Line 2   Filling   Sanitation   01 ufc   02 ufc   —                   Valve   Water       467   15 Aug. 2002   Line 2   Filling   Sanitation   04 ufc   03 ufc   —                   Valve   Water       468   15 Aug. 2002   Line 2   Filling   Sanitation   05 ufc   08 ufc   —                   Valve   Water       469   15 Aug. 2002   Line 2   Filling   Sanitation   09 ufc   08 ufc   —                   Valve   Water       470   15 Aug. 2002   Line 2   Filling   Sanitation   04 ufc   03 ufc   —                   Valve   Water       471   15 Aug. 2002   Line 2   Filling   Sanitation   05 ufc   04 ufc   —                   Valve   Water       472   15 Aug. 2002   Line 2   Filling   Sanitation   05 ufc   05 ufc   —                   Valve   Water       473   15 Aug. 2002   Line 2   Filling   Sanitation   03 ufc   05 ufc   —                   Valve   Water       474   15 Aug. 2002   Line 2   Filling   Sanitation   03 ufc   03 ufc   —                   Valve   Water       475   16 Aug. 2002   Line 2   Filling   Sanitation   04 ufc   06 ufc   —                   Valve   Water       476   16 Aug. 2002   Line 2   Filling   Sanitation   05 ufc   05 ufc   —                   Valve   Water       477   16 Aug. 2002   Line 2   Filling   Sanitation   05 ufc   07 ufc   —                   Valve   Water       478   16 Aug. 2002   Line 2   Filling   Sanitation   09 ufc   07 ufc   —                   Valve   Water       479   17 Aug. 2002   Line 2   Syrup   Sanitation   08 ufc   05 ufc   —                   Tank   Water       480   17 Aug. 2002   Line 2   Beverage   Sanitation   07 ufc   07 ufc   —                   Tank   Water       481   6 Sep. 2002   Syrup   Tank JT 4   Sanitation   00 ufc   00 ufc   —               Room       Water       482   6 Sep. 2002   Syrup   Tank JT 3   Sanitation   01 ufc   00 ufc   —               Room       Water       483   6 Sep. 2002   Syrup   Tank JT 2   Sanitation   01 ufc   01 ufc   —               Room       Water       484   6 Sep. 2002   Syrup   Tank JT 1   Sanitation   01 ufc   00 ufc   —               Room       Water       485   6 Sep. 2002   Syrup   Tank JT 5   Sanitation   00 ufc   00 ufc   —               Room       Water       486   6 Sep. 2002   Syrup   Tank JT 1   Sanitation   00 ufc   00 ufc   —               Room       Water       487   6 Sep. 2002   Syrup   Tank JT 11   Sanitation   00 ufc   02 ufc   —               Room       Water       488   7 Sep. 2002   Syrup   Tank JT 6   Sanitation   02 ufc   01 ufc   —               Room       Water       489   7 Sep. 2002   Syrup   Tank JT 9   Sanitation   00 ufc   00 ufc   —               Room       Water       490   7 Sep. 2002   Syrup   Tank JT 4   Sanitation   00 ufc   00 ufc   —               Room       Water       491   7 Sep. 2002   Syrup   Tank JT 3   Sanitation   01 ufc   02 ufc   —               Room       Water       492   7 Sep. 2002   Syrup   Tank JT 1   Sanitation   00 ufc   00 ufc   —               Room       Water       493   7 Sep. 2002   Syrup   Tank JT 11   Sanitation   00 ufc   01 ufc   —               Room       Water       494   9 Sep. 2002   Syrup   Tank JT 5   Sanitation   00 ufc   00 ufc   —               Room       Water       495   9 Sep. 2002   Syrup   Tank JT 10   Sanitation   00 ufc   00 ufc   —               Room       Water       496   9 Sep. 2002   Syrup   Tank JT 3   Sanitation   01 ufc   00 ufc   —               Room       Water       497   9 Sep. 2002   Syrup   Tank JT 1   Sanitation   00 ufc   01 ufc   —               Room       Water       498   10 Sep. 2002   Syrup   Tank JT 4   Sanitation   00 ufc   00 ufc   —               Room       Water       499   10 Sep. 2002   Syrup   Tank JT 5   Sanitation   03 ufc   01 ufc   —               Room       Water       500   10 Sep. 2002   Syrup   Tank JT 6   Sanitation   01 ufc   00 ufc   —               Room       Water                  
 
         [0057]    The underlined tests in the above Table 4 correspond to inefficiencies in the preparation of the three culture mediums.  
         [0058]    The culture medium composition of the invention keeps its characteristics and effectiveness after 6 months at 4 a C and therefore it may be considered that the inventive composition has a long life, thus allowing to work with high production indexes.  
         [0059]    Conclusions:  
         [0060]    1. The culture medium composition of the invention allows the production costs of culture mediums used for quality controls to decrease because of the low costs of its components as compared to other commercial culture mediums.  
         [0061]    2. The components of the medium rapidly dissolve in an aqueous medium and the obtained composition does not require a filtration step for eliminating impurities.  
         [0062]    3. The pH is easily adjusted.  
         [0063]    4. The preparation of the inventive composition takes a short time.  
         [0064]    5. The colonies grown in the composition are small thus making the counting thereof easier.  
         [0065]    6. The composition may be prepared in large volumes and it is easily sterilized by techniques like autoclave, filtration.  
         [0066]    7. The composition has an average life of one year at 4 a C.  
         [0067]    This invention is better illustrated in the following examples that should not be construed as restrictive to the scope of the invention. On the contrary, it should be clearly understood that, after reading the present disclosure, other embodiments, modifications and equivalents of the invention will be apparent to those persons skilled in the art without departing from the spirit of the invention and/or the scope of the appended claims.  
       EXAMPLE 1  
       [0068]    Method of Elaborating the Culture Medium Composition of the Invention.  
         [0069]    5.0 g of monobasic potassium phosphate, 1.0 of ammonium chloride, 1.0 g of heptahydrate magnesium sulfate and 40.0 g of D(+) saccharose have been mixed in a precipitate glass having a capacity for 1000 ml of final solution. Ultra pure water has been added up to 1 litre volume. The solution has been stirred or agitated by a magnetic stirrer up to having the components dissolved.  
         [0070]    While maintaining a constant stirring a pre-calibrated and normalized electrode was introduced into the solution. The pH of the medium was measured and adjusted to a final 5 value by dripping a 1N sodium hydroxide solution.  
         [0071]    The resulting solution was poured into an autoclave resistant flask and the flask was properly sealed. The medium was sterilized in autoclave at a temperature of 121° C. for 15 min.  
         [0072]    After the sterilization in autoclave the medium was conserved refrigerated at a temperature of about 4° C.  
       EXAMPLE 2  
       [0073]    Comparative Analysis of the Efficiency of the Inventive Composition and Conventional Culture Mediums.  
         [0074]    Fungi and yeast colonies have been dispersed in 500 ml ultra pure water and the following dilutions have been made 1/10 2 , 1/10 4 , 1/10 6 , 1/10 8 , 1/10 10 , 1/10 12 , 1/10 14 , 1/10 16 , 1/10 18 , 1/10 20 .  
         [0075]    In addition 12 Petri plates have been prepared for each culture medium. Each plate contained 2.5 ml of culture medium placed into the culture plate that had an absorbent surface “pad”.  
         [0076]    20 ml of each sample were spread into each plate and incubated at 28° C. for 72 hours.  
         [0077]    Comparative analysis were then carried out of samples taken from the sanitation water from the filling valves, as it is explained below.  
         [0078]    Analysis of the sanitation samples:  
         [0079]    After finishing the sanitation of the production line bacteriologic samples have been drawn of the final sanitation rinsing water. The samples have been taken from the filling valves after blazing thereof and the samples were placed into 120 ml sterile flasks. Then the samples were filtrated through a membrane with 0.8 micron pores, under entire aseptic conditions. The filters were removed and placed into Petri plates with pads and containing 3 ml of corresponding culture medium. Each plate was maintained for 72 hours at 28° C. and the colonies were then counted.  
         [0080]    Comparative analysis of fungi-contaminated samples were also carried out with such samples having different contamination levels or microorganisms contents.  
         [0081]    To that purpose samples of water intentionally contaminated with fungi at high concentrations (higher than 400 ufc), half concentrations (close to 100 ufc) and low concentrations (below 10 ufc) have been prepared. The samples were filtrated through a membrane with 0.8 micron pores. The filters were removed and placed into Petri plates with pads and containing 3 ml of each culture medium. The plates were maintained at 28° C. for 72 hours. After the incubation period the plates were removed and the colonies were counted.  
       EXAMPLE 3  
       [0082]    Study of the Life of the Inventive Composition.  
         [0083]    A flask containing 1000 ml of the inventive culture composition was placed in a room at 4° C., with the room properly sterilized.  
         [0084]    Time tests of the life of the inventive composition were simultaneously carried out. To this purpose the culture medium was fractioned into 2.5 ml vials that were sterilized and conserved at 4° C. The vials were removed one by one for 6 months and their effectiveness for culturing fungi and yeast were tested. The test in Petri plates was the same disclosed in the prior Example. Samples intentionally contaminated with fungi and yeast were employed as positive controls, and membranes through which the sterile culture medium was passed were employed as negative controls.  
         [0085]    While preferred embodiments of the present invention have been illustrated and described, it will be obvious to those skilled in the art that various changes and modifications may be made therein without departing from the scope of the invention as defined in the appended claims.