Clinical trial NCT06403241: The Association Between Food Timing and Composition on Gastrointestinal Injury, Stress Hormone and Gastrointestinal Complains.
Official title: The Association Between Food Timing and Composition on Gastrointestinal Injury, Stress Hormone and Gastrointestinal Complains in Elite Rowers After 2000m Ergometer Test - an Observational Study.
Study type: OBSERVATIONAL
Overall status: COMPLETED
Conditions: Endothelial Dysfunction
Keywords: athlete's diet
endotoxemia
cortisol
gut injury
gut permeability
exercise
Lead sponsor: Poznan University of Physical Education
Sponsor class: OTHER
First posted: 2024-05-07
Enrollment: 13
Sex: MALE
Minimum age (years): 18
Maximum age (years): 23
Standard ages: ADULT
Accepts healthy volunteers: yes
Observational model: COHORT
Time perspective: RETROSPECTIVE
Sampling method: NON_PROBABILITY_SAMPLE
Study population: Male rowers age 18 - 22 years.

Brief summary: The study aimed to check the influence of timing and composition of diet on markers of gut injury, endotoxemia and stress.

Detailed description: The research protocol includes detailed food intake analysis, including timing and composition, markers related to the neuroendocrine pathway cortisol, and observations of individually experienced symptoms. Moreover, the study also explores intestinal injury and endotoxemia parameters, such as I-FABP, CLDN-3, and LBP. As a first goal, this study investigates the timing and composition of food intake concerning changes in gut integrity, cortisol levels, and gastrointestinal symptoms. Investigators explored whether an athlete's nutritional intake influences any GI disturbance induced by high-intensity exercise. As a second goal, Investigators tried to connect symptoms and markers from both pathways with gastrointestinal complaints. Investigators hypothesised that cortisol levels would be more profound than other studied parameters in gastrointestinal symptoms.

Eligibility criteria: Inclusion Criteria:

* Membership in Polish Rowing Team,
* minimum five years of training,
* total training time minimum of 240 minutes,
* filling out a food diary,
* finishing 2000-meter ergometer test.

Exclusion Criteria:

* Probiotics within the last three months,
* prebiotics within the last three months
* antibiotic therapy within the last three months,
* dietary regime,
* gastrointestinal diseases,
* lactose intolerance

Primary outcomes:
measure: I-FABP (intestinal fatty acid binding protein)to measure epithelial wall injury
timeFrame: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting) up to one hour after getting up, 1 minute after the test, and 1-hour recovery period
measure: Claudin to measure tight junction leakage
timeFrame: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting) up to one hour after getting up, 1 minute after the test, and 1-hour recovery period
measure: LBP (lipopolysaccharide binding protein) to measure endotoxin
timeFrame: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting) up to one hour after getting up, 1 minute after the test, and 1-hour recovery period
measure: Lactic acid to measure fatigue after the race
timeFrame: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting) up to one hour after getting up, 1 minute after the test
measure: Corisol
timeFrame: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting) up to one hour after getting up, 1 minute after the test, and 1-hour recovery period
Secondary outcomes:
measure: energy
timeFrame: whole day (24hours) before test, in the morning before the test
measure: protein
timeFrame: whole day (24hours) before test, in the morning before the test
measure: fat
timeFrame: whole day (24hours) before test, in the morning before the test
measure: carbohydrate
timeFrame: whole day (24hours) before test, in the morning before the test
measure: glucose
timeFrame: whole day (24hours) before test, in the morning before the test
measure: lactose
timeFrame: whole day(24hours) before test, in the morning before the test
measure: saccharose
timeFrame: whole day (24hours) before test, in the morning before the test
measure: fructose
timeFrame: whole day (24hours) before test, in the morning before the test
measure: fiber
timeFrame: whole day (24hours) before test, in the morning before the test
measure: Body mass
timeFrame: in the morning before the test up to 1 hour after getting up
measure: Body fat
timeFrame: in the morning before the test up to 1 hour after getting up
measure: Total body water
timeFrame: in the morning before the test up to 1 hour after getting up
measure: Lean body mass
timeFrame: in the morning before the test up to 1 hour after getting up
measure: height
timeFrame: in the morning before the test up to 1 hour after getting up
measure: self reported gastrointestinal scale
timeFrame: up to 1 hours after the test
Other outcomes:
Locations:
city: Gorzów Wielkopolski
country: Poland
facility: Poznan University of Physical Education, Faculty of Sport Sciences in Gorzow
state: Lubuskie
Study officials:
affiliation: Poznan University of Physical Education
name: Anna Skarpańska-Stejnborn, professor
role: STUDY_DIRECTOR
References: