Clinical trial NCT02645149: Molecular Profiling and Matched Targeted Therapy for Patients With Metastatic Melanoma
Official title: Molecular Profiling and Matched Targeted Therapy for Patients With Metastatic Melanoma
Study type: INTERVENTIONAL
Overall status: RECRUITING
Phase: PHASE2
Conditions: Melanoma
Keywords: Metastatic Melanoma
Molecular Testing
Targeted Therapy
Lead sponsor: Melanoma Institute Australia
Sponsor class: OTHER
Collaborators: name: Novartis
First posted: 2016-01-01
Enrollment: 1000
Sex: ALL
Minimum age (years): 18
Maximum age (years): 100
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: no
Allocation: NON_RANDOMIZED
Intervention model: SINGLE_GROUP
Primary purpose: TREATMENT

Brief summary: This is a patient oriented translational research project aiming to improve clinical outcomes for patients with BRAF and NRAS wild-type unresectable Stage III or Stage IV metastatic melanoma who have progressed on, or are unable to receive standard therapy (in general, immunotherapy). Consecutive patients seen at three major clinics and fitting the broad eligibility criteria will be invited to participate.

The approach is designed to test the impact of different targeted drugs on different mutations in a single type of cancer. In this project, patients will have tumour tissue genetically profiled to determine which mutation(s) are present, and will then be assigned to receive a matched drug expected to target the mutation(s) in the tumour. Where multiple targets are identified in one patient, or where multiple potential therapies would be appropriate for a single tumour mutation, the treating clinician may determine the appropriate therapeutic approach after consultation with the study team, using the latest version of library of matched therapies.

Detailed description: Current standard of care testing covers mutations in the BRAF and NRAS genes. The comprehensive gene testing platform to be used in this project is designed to interrogate the entire coding sequence of 315 cancer-related genes plus introns from 28 genes often rearranged or altered in cancer. These genes are known to be somatically altered in solid cancers based on recent scientific and clinical literature. The test panel will be used on melanoma tissue from patients pre-screened as having BRAF and NRAS wild-type melanoma. The extent of testing will be updated to reflect new discoveries in melanoma tumourigenesis and availability of new therapies as the project proceeds. Testing will be performed on formalin-fixed and paraffin-embedded samples of metastatic tissue. Archival tissue from primary or regional recurrent melanoma may be considered if no recent sample is available or possible.

All new patients with unresectable Stage III or IV melanoma seen at each participating site will be invited to participate. Following written consent, all patients will undergo the standard testing for BRAF and NRAS mutations. Patients found to have mutations in either gene will receive standard treatment with dabrafenib and / or trametinib and no further molecular testing.

Patients with wild type versions of BRAF and NRAS genes will have tumour tissue sent for the extended gene testing platform. These patients will first receive standard of care therapy for wild type BRAF / NRAS melanoma, where possible. Once standard therapies have been exhausted (because of disease progression or intolerable adverse events), patients will then receive a targeted therapy matched for their genetic result, if applicable. The selection of targeted therapy will be based on clinical evidence of activity in other solid tumours harbouring similar mutations and / or in at least Phase I testing in melanoma. The table of matched therapies will be modified and extended as new findings from clinical research become available. Patients may be included in specific clinical trials of targeted therapies if available, e.g. a MEK inhibitor combined with an MDM2 inhibitor (AMG232) for BRAF and TP53 wild-type melanoma or a MEK inhibitor combined with a CDK4/6 inhibitor for BRAF wild-type melanoma.

The extensive molecular profiling platform is fully validated and will be conducted by an external provider accredited by an authority with the responsibility to award Laboratory Accreditation at private and public facilities.

Eligibility criteria: Inclusion criteria for Inclusion in Molecular Testing Platform:

1. Newly diagnosed and treatment naïve unresectable Stage IIIB, IIIC or Stage IV melanoma (including sub types: cutaneous, mucosal, acral, ungual, uveal and unknown primary).
2. Archival metastatic tumour tissue available for genetic testing. Archival tissue from primary melanoma may be considered if no recent sample is available.
3. Male or female patients aged 18 or over.
4. Written informed consent for molecular genetic testing of tumour tissue (for both standard and research tests).

Inclusion Criteria for Matched Targeted Therapy:

6. Received available standard therapies for metastatic melanoma and progressed, unable to tolerate standard therapy, or standard therapy contraindicated.

7. Written informed consent to receive targeted therapy (if applicable) and clinical follow up.

8. Patient has an 'actionable' genetic aberration and matched targeted therapy is available. Patients with no genetic aberration or where no matched targeted therapy is available, patients will be offered trametinib 9. ECOG status 0 - 2. 10. Adequate haematological, hepatic and renal organ function as defined by:

1. White cell count ≥ 2.0 × 109/L
2. Neutrophil count ≥ 1.5 × 109/L
3. Haemoglobin ≥ 90 g/L
4. Platelet count ≥ 100 x 109/L
5. Total bilirubin ≤ 3.0 x ULN
6. Alanine transaminase ≤ 3.0 x ULN
7. Aspartate aminotransferase ≤ 3.0 x ULN
8. Serum creatinine ≤ 1.5 x the upper limit of normal (ULN). 11. Life expectancy \> 30 days. 12. Women of child bearing potential (WOCBP) to use contraception to avoid pregnancy.

   13. Non sterile men with female partners of CBP to use contraception to avoid pregnancy.

   14. Drug specific inclusions.

   Exclusion criteria for Matched Targeted Therapy:
   1. An expectation for the need for concurrent radiotherapy (unless safety has been established with the matched drug regimen and is directed at one anatomical region for symptom control).
   2. Any investigational drug or other systemic drug therapy for melanoma within 14 days or 5 half-lives from baseline, whichever is shorter.
   3. Pregnant or breast feeding females.
   4. Drug specific exclusions.
   5. Any clinically significant gastrointestinal abnormalities which may impair intake or absorption of the study drug

Primary outcomes:
measure: Type and frequency of genetic aberrations in BRAF/NRAS wild-type metastatic melanoma
timeFrame: For the duration of the study, estimated at 5 years.
measure: Proportion of patients with BRAS/NRAS wild-type melanoma receiving targeted therapy
timeFrame: For the duration of the study, estimated at 5 years.
Secondary outcomes:
measure: Proportion of patients who have BRAF/NRAS wild type melanoma
timeFrame: For the duration of the study, estimated at 5 years.
measure: Proportion of patients with complete (CR) or partial (PR) response.
timeFrame: From date of targeted therapy commencement until the date of first documented objective partial or complete response per RECIST, assessed up to 60 months.
measure: Duration of response
timeFrame: From date of first objective partial or complete response to disease progression or death, which ever is sooner, assessed up to 60 months.
measure: Progression free survival
timeFrame: From date of targeted therapy commencement to date of objective disease progression per RECIST, assessed up to 60 months.
measure: Overall survival
timeFrame: From date of targeted therapy commencement to date of death from any cause, assessed up to 60 months.
measure: Correlation of genetic aberration detected in tumour tissue with clinical response and disease progression
timeFrame: From date of targeted therapy commencement to date of to partial or complete response or disease progression, assessed up to 60 months.
measure: Correlation of with genetic aberration detected in tumour tissue with age at diagnosis, site of primary tumour, chronic sun damaged skin.
timeFrame: At baseline
measure: Correlation of genetic aberration detected in tumour tissue with site of metastases, nodular or superficial spreading disease.
timeFrame: At baseline
measure: Adverse events in patients receiving matched targeted therapy.
timeFrame: From date of the start of targeted therapy to 30 days from discontinuation of targeted therapy
Other outcomes:
Locations:
city: Westmead
country: Australia
facility: Westmead Hospital
state: New South Wales
city: Wollstonecraft
country: Australia
facility: Melanoma Institute Australia
state: New South Wales
Study officials:
affiliation: Melanoma Institute Australia
name: Alex Menzies
role: STUDY_CHAIR
References:
citation: Nassar KW, Hintzsche JD, Bagby SM, Espinoza V, Langouet-Astrie C, Amato CM, Chimed TS, Fujita M, Robinson W, Tan AC, Schweppe RE. Targeting CDK4/6 Represents a Therapeutic Vulnerability in Acquired BRAF/MEK Inhibitor-Resistant Melanoma. Mol Cancer Ther. 2021 Oct;20(10):2049-2060. doi: 10.1158/1535-7163.MCT-20-1126. Epub 2021 Aug 10.