Clinical trial NCT05199259: Multi-analyte Blood Test Clinical Trial
Official title: Collection of Blood to Evaluate Epigenomics and Protein Biomarkers for the Detection of Hepatocellular Carcinoma
Study type: OBSERVATIONAL
Overall status: RECRUITING
Conditions: Liver Cirrhosis
Liver Cancer
HCC
Hepatocellular Carcinoma
Lead sponsor: Helio Genomics
Sponsor class: INDUSTRY
First posted: 2022-01-20
Enrollment: 1200
Sex: ALL
Minimum age (years): 18
Standard ages: ADULT
OLDER_ADULT
Observational model: OTHER
Time perspective: PROSPECTIVE
Sampling method: NON_PROBABILITY_SAMPLE
Study population: The subjects will fall into two main groups, subjects diagnosed with HCC (HCC positive Group) or subjects without HCC (HCC negative Group). The HCC negative Group will be further divided into two sub-groups based on whether the absence of HCC has been determined using CT or MRI procedures (Sub-group 1) or ultrasound (Sub-group 2). Only the subjects in sub-group 2 will receive a confirmatory ultrasound approximately 6 months (between 5 to 7 months) after enrollment to confirm the absence of HCC ("6-month visit). This additional imaging study is necessary due to the low sensitivity of abdominal ultrasound to detect HCC lesions. The blood specimens will be shipped to a clinical diagnostic laborator

Brief summary: The objective of this study is the acquisition of whole blood samples and serum samples from participants with untreated Hepatocellular Carcinoma (HCC) and subjects undergoing Hepatocellular Carcinoma (HCC) surveillance. These samples will be used for research purposes to develop and validate the Helio multi-analyte blood test.

Detailed description: This study pertains to the collection of whole blood and serum specimens from participants undergoing Hepatocellular Carcinoma (HCC) surveillance. The participants will fall into two main groups, subjects diagnosed with HCC (HCC positive Group) or subjects without HCC (HCC negative Group).

The HCC negative Group will be further divided into two sub-groups based on whether the absence of HCC has been determined using CT or MRI procedures (Sub-group 1) or ultrasound (Sub-group 2). Only the participants in sub-group 2 will receive a confirmatory ultrasound approximately 6 months (between 5 to 9 months) after enrollment to confirm the absence of HCC (6-month visit). This additional imaging study is necessary due to the low sensitivity of abdominal ultrasound to detect HCC lesions.

Participants will be screened for eligibility to participate in the study based on their medical history and records. Participants with a recent confirmed Collection of Blood to Evaluate Epigenomics and Protein Biomarkers for the detection of Hepatocellular Carcinoma diagnosis of HCC (within 6 months of enrollment) may be enrolled in such way to ensure the cases are representative of the major liver disease etiologies in the surveillance population in the United States.

Specifically, the following causes of cirrhosis will be selected:

* Alcoholic steatohepatitis (ASH);
* Hepatitis B virus (HBV);
* Hepatitis C virus (HCV);
* Non-alcoholic fatty liver disease (NAFLD);
* Other genetic conditions that cause cirrhosis (i.e., hemochromatosis)

These blood samples will be used to perform various studies to determine the utility of selected DNA methylation and protein markers for the liver cancer diagnostic test.

Eligibility criteria: Inclusion Criteria:

* Age 18 years or older.
* Males and Females.
* Having cirrhosis or meeting the AASLD guidelines for HCC
* surveillance.
* Clinically diagnosed with HCC or negative for HCC following disease
* surveillance.
* HCC positive Group: Subject has a recent (within 6 months of enrollment) clinically diagnosed, untreated hepatocellular carcinoma as defined by at least one ≥1 cm lesion exhibiting arterial phase hyperenhancement in combination with washout appearance and/or capsule by 4 phase CT scan or multiphase contrast enhanced MRI or biopsy is positive for HCC.
* HCC negative Group: Non-cancer, at-risk subjects with chronic liver disease undergoing routine imaging surveillance for HCC, where the definitive lack of HCC within 3 months prior to enrollment has been verified by negative imaging, for HCC. No more than 200 subjects without cirrhosis can be enrolled in this group.
* Sub-Group 1 (approximately 450 subjects) - negative by CT or MRI (No lesion, LR-1 or LR-2)
* Sub-Group 2 (approximately 450 subjects) - negative by ultrasound

Exclusion Criteria:

* Subjects that are unwilling or unable to sign the Informed Consent Form will be excluded.
* Known cancer diagnosis of a cancer other than HCC within the past 5 years (with the exceptions of basal cell or squamous cell skin cancers).
* Chemotherapy and/or radiation therapy within 5 years prior to enrollment/sample collection.
* Prior or current treatment with sorafenib, regorafenib, or other treatment indicated for HCC.
* Prior treatment with a DNA methyltransferase inhibitor such as with Vidaza (azacitidine) or Dacogen (decitabine)
* Any HCC treatment prior to enrollment/blood sample collection (e.g., surgery, ablation, embolization, pharmacotherapy, radiotherapy, liver transplant or other treatment indicated for HCC).
* IV contrast (e.g., CT and MRI) within 1 day \[or 24 hours\] of blood collection.
* Less than 3 days between fine needle aspiration (FNA) of target pathology and blood collection.
* Less than 7 days between biopsy (other than FNA) of target pathology and blood collection.
* Any condition the Investigator believes would interfere with his or her ability to provide informed consent, comply with the study protocol, which might confound the interpretation of the study results or put the person at undue risk.
* For HCC negative subjects, patients with a prior diagnosis of HCC are also excluded.
* Subjects that are pregnant will be exclude

Primary outcomes:
measure: Independent performance measure of sensitivity and specificity of a multi-analyte blood test
timeFrame: 1 - 9 months
Secondary outcomes:
measure: To investigate potential endogenous and exogenous interfering substances of a multi-analyte blood test
timeFrame: 1 - 9 months
measure: Ascertain Reference Range(s)
timeFrame: 1 - 9 months
measure: Ascertain Sample Stability
timeFrame: 1 - 9 months
Other outcomes:
Locations:
city: Mission Hills
country: United States
facility: Providence Facey Medical Foundation
state: California
city: Tampa
country: United States
facility: Guardian Angel Research Center
state: Florida
city: Baltimore
country: United States
facility: GI Research Mercy Medical Center
state: Maryland
city: Edinburg
country: United States
facility: South Texas Research Institute
state: Texas
city: El Paso
country: United States
facility: Texas Gastro Research
state: Texas
city: Waco
country: United States
facility: Impact Research Institute
state: Texas
city: Norfolk
country: United States
facility: Digestive & Liver Disease Specialist
state: Virginia
Study officials:
affiliation: Helio Health
name: Taggert
role: STUDY_DIRECTOR
References: