Clinical trial NCT06787807: Proteomic Analysis of Patients Undergoing Bariatric Surgery
Official title: Proteomic Analysis of Patients Undergoing Bariatric Surgery
Study type: INTERVENTIONAL
Overall status: NOT_YET_RECRUITING
Phase: NA
Conditions: Obesity
Colon Cancer
Thyroid Cancer
Esophageal Cancer
Pancreatic Cancer
Gastric Cancer
Potmenopausal Breast Cancer
Endometrial Cancer
Liver Cancer
Gallbladder Cancer
Prostate Cancer
Ovarian
Kidney Cancer
Multiple Myeloma
Meningioma
Breast Cancer
Ovarian Cancer
Keywords: obesity
cancer
bariatric surgery
proteomics
biomarkers
mass spectrometry
Lead sponsor: Universidade Federal de Pernambuco
Sponsor class: OTHER
First posted: 2025-01-22
Enrollment: 103
Sex: ALL
Minimum age (years): 18
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: yes
Allocation: NA
Intervention model: SINGLE_GROUP
Primary purpose: PREVENTION

Brief summary: The expression of proteins in the blood of obese individuals is different from the expression of proteins in healthy individuals, and is also different in individuals after bariatric surgery. Therefore, this research aims to better understand protein expression in patients indicated for surgical treatment of obesity, evaluating the pre-operative and post-operative status. The objective of this research is to better understand protein expression in patients indicated for surgical treatment of obesity, evaluating the pre-operative and post-surgery status.

Detailed description: The objective of this study is to analyze the Proteomics of obese patients in the preoperative context of bariatric surgery to evaluate which are the up-regulated proteins - potentially related to oncogenic metabolic pathways - and also the down-regulated proteins in the context of obesity - potentially protective in the context of cancer. As well as, evaluate the variation of these proteins, as well as the metabolic pathways with which they are associated, after weight loss promoted by bariatric surgery, in the period of 01 year after this intervention.

The proteomic study will be carried out based on the formation of pools organized based on the patients' pre- and postoperative similarities. Proteomic analyzes will be carried out on a high-performance liquid chromatography system (nanoUPLC) model Acquity Mclass, Waters. Coupled to a Quadrupole Mass Spectrometer-Time of Flight (QToF) Model Sunapt Xs, Waters. Finally, the raw data (raw mass spectra) will be analyzed by the PROGENESIS QI software (Waters) to identify the proteins.

Eligibility criteria: Inclusion Criteria:

* patients over 18 years of age with a BMI above 35 kg/m² associated with comorbidity or BMI above 40 kg/m² without comorbidities,

Exclusion Criteria:

* Patients with a previous history of cancer;
* Underage patients
* Patients with other diseases predisposing to the development of cancer: cirrhosis, ulcerative colitis, Barrett's esophagus, etc.
* Patients who have previously undergone bariatric surgery;

Primary outcomes:
measure: Number of patients with reduced initially upregulated oncogenic proteins
timeFrame: 1 year after bariatric surgery
Secondary outcomes:
Other outcomes:
Locations:
city: Recife
country: Brazil
facility: Hospital das clínicas da UFPE
state: Pernambuco
Study officials:
affiliation: UFPE
name: Álvaro B Ferraz, Doctor
role: PRINCIPAL_INVESTIGATOR
References: