Clinical trial NCT01728675: Eccentric Exercise and Oxidative Stress
Official title: The Effect of Eccentric Exercise on Oxidative Stress in the Elderly
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Aging
Muscle Damage
Oxidative Stress
Keywords: Aging
Skeletal muscle
Biomarkers
Adaptation
Lead sponsor: European University Cyprus
Sponsor class: OTHER
Collaborators: name: Aristotle University Of Thessaloniki
First posted: 2012-11-20
Enrollment: 20
Sex: MALE
Minimum age (years): 18
Maximum age (years): 70
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: yes
Allocation: NON_RANDOMIZED
Intervention model: PARALLEL
Primary purpose: SUPPORTIVE_CARE

Brief summary: The purpose of the present investigation is to compare the responses and adaptations of young and elderly individuals to repeated eccentric exercise in regards to muscle function and redox homeostasis in a side-by-side comparison.

Detailed description: Despite the progress of analytic techniques and the refinement of study designs, striking disagreement exists among studies regarding the influence of exercise on muscle function and redox homeostasis in the elderly. A specific exercise protocol will be applied (the repeated eccentric model) to produce long-lasting and extensive changes in redox biomarkers and to examine more easily the potential effects of aging. Ten young men and ten elderly men will underwent an isokinetic eccentric exercise session, which will be repeated after three weeks. Muscle function indices and redox biomarkers will be assessed in urine, plasma or erythrocytes pre exercise, immediately post exercise, 2 and 4 days post exercise.

Eligibility criteria: Inclusion Criteria:

* subject provides written informed consent
* physiological health profile
* stable at their anthropometric characteristics for at least the last year

Exclusion Criteria:

* muscle disease
* prior history of musculoskeletal injury to the lower limbs
* smokers
* consumed any nutritional supplement the last 3 months
* performed intense eccentric exercise the last 6 months
* non Caucasian

Primary outcomes:
measure: Muscle torque (torque)
timeFrame: Change in muscle torque from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
measure: Range of motion, ROM (degrees)
timeFrame: Change in ROM from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
measure: Delayed onset muscle soreness, DOMS (scale 1-10)
timeFrame: Change in DOMS from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
measure: Creatine kinase, CK (activity IU)
timeFrame: Change in CK from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
Secondary outcomes:
measure: F2-isoprostanes (pg/mg creatinine)
timeFrame: Change in F2-isoprostanes from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
measure: Protein carbonyls (nmol/mg protein)
timeFrame: Change in F2-isoprostanes from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
measure: Glutathione, GSH (μmol/g Hb)
timeFrame: Change in GSH from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
measure: Catalase (U/g Hb)
timeFrame: Change in catalase from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
measure: Superoxide dismutase, SOD (U/g Hb)
timeFrame: Change in SOD from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
measure: Glutathione peroxidase, GPx (U/g Hb)
timeFrame: Change in GPx from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
measure: Glucose-6-phosphate dehydrogenase, G6PD (U/g Hb)
timeFrame: Change in G6PD from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
measure: Uric acid (mM)
timeFrame: Change in uric acid from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
measure: Albumin (μM)
timeFrame: Change in albumin from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
measure: Bilirubin (μM)
timeFrame: Change in bilirubin from baseline (pre-exercise) at immediately after exercise and at day 2 and day 4 post-exercise
Other outcomes:
Locations:
city: Nicosia
country: Cyprus
facility: European University Cyprus
Study officials:
affiliation: Aristotle University Of Thessaloniki
name: Michalis G Nikolaidis, PhD
role: STUDY_CHAIR
References:
citation: Nikolaidis MG, Kyparos A, Vrabas IS. F(2)-isoprostane formation, measurement and interpretation: the role of exercise. Prog Lipid Res. 2011 Jan;50(1):89-103. doi: 10.1016/j.plipres.2010.10.002. Epub 2010 Oct 15.
citation: Nikolaidis MG, Jamurtas AZ. Blood as a reactive species generator and redox status regulator during exercise. Arch Biochem Biophys. 2009 Oct 15;490(2):77-84. doi: 10.1016/j.abb.2009.08.015. Epub 2009 Aug 25.
citation: Nikolaidis MG, Kyparos A, Spanou C, Paschalis V, Theodorou AA, Vrabas IS. Redox biology of exercise: an integrative and comparative consideration of some overlooked issues. J Exp Biol. 2012 May 15;215(Pt 10):1615-25. doi: 10.1242/jeb.067470.
citation: Theodorou AA, Nikolaidis MG, Paschalis V, Sakellariou GK, Fatouros IG, Koutedakis Y, Jamurtas AZ. Comparison between glucose-6-phosphate dehydrogenase-deficient and normal individuals after eccentric exercise. Med Sci Sports Exerc. 2010 Jun;42(6):1113-21. doi: 10.1249/MSS.0b013e3181c67ecd.
citation: Panayiotou G, Paschalis V, Nikolaidis MG, Theodorou AA, Deli CK, Fotopoulou N, Fatouros IG, Koutedakis Y, Sampanis M, Jamurtas AZ. No adverse effects of statins on muscle function and health-related parameters in the elderly: an exercise study. Scand J Med Sci Sports. 2013 Oct;23(5):556-67. doi: 10.1111/j.1600-0838.2011.01437.x. Epub 2012 Jan 31.