Clinical trial NCT04566354: Interleukin-6 Secretion in Adipose Tissue Following Sprint Exercise
Official title: Metabolism in Adipose Tissue Followed Following Sprint Exercise
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Metabolism in Adipose Tissue
Lead sponsor: Karolinska Institutet
Sponsor class: OTHER
First posted: 2020-09-28
Enrollment: 18
Sex: ALL
Minimum age (years): 20
Maximum age (years): 40
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: NA
Intervention model: SINGLE_GROUP
Primary purpose: BASIC_SCIENCE

Brief summary: Low volume sprint exercise training has been shown to reduce body fat despite small total energy expenditure (for ref see Gillen and Gibala 2014). IL-6 has been shown to increase adipose tissue lipolysis in vitro. Therefore, in an initial study on effects of sprint exercise on adipose tissue metabolism the investigators will examine acute short-term exercise effects on adipose tissue IL-6 exchange.

Detailed description: PURPOSE: Low volume sprint exercise training has been shown to reduce body fat despite small total energy expenditure (for ref see Gillen and Gibala 2014). IL-6 has been shown to increase adipose tissue lipolysis in vitro. Therefore, in an initial study on effects of sprint exercise on adipose tissue metabolism the investigators examined acute short-term effects on adipose tissue IL-6 exchange.

METHODS: Four female and four male subjects perform repeated sprint exercise (3 x 30s with 20 min rest between; Wingate-test). Blood samples are repeatedly obtained, up to 120 min after the last sprint (9 min post ex), from catheters inserted percutaneously into brachial artery and a superficial subcutaneous vein on the anterior abdominal wall providing access to the venous drainage from the subcutaneous adipose tissue and analyzed for IL-6 by ELISA and lactate by spectrophotometry. Fat biopsies from the stomach will be obtained before, 15 min and 120 min after the last sprint. Blood flow will be determined by administration of Xenon-133 in the adipose tissue of the stomach. The release rate of Xenon-133 will be registrated continuously during the total investigation.

SUPPORT: This study is supported by grants from the Swedish National Center for Research in Sports

Eligibility criteria: Inclusion Criteria:

* Physical active
* Healthy

Exclusion Criteria:

* Smokers
* Pregnant
* On medication
* No infection
* No asthma

Primary outcomes:
measure: Il-6 secretion in adipose tissue before and after 30-s repeated sprint exercise
timeFrame: Before 30-s repeated sprint exercise
measure: Il-6 secretion in adipose tissue before and after 30-s repeated sprint exercise
timeFrame: After 30-s repeated sprint exercise
Secondary outcomes:
measure: Changes in blood flow in adipose tissue
timeFrame: Before 30-s repeated sprint exercise
measure: Changes in blood flow in adipose tissue
timeFrame: After 30-s repeated sprint exercise
measure: Changes in different metabolites
timeFrame: Before 30-s repeated sprint exercise
measure: Changes in different metabolites
timeFrame: After 30-s repeated sprint exercise
Other outcomes:
Locations:
city: Stockholm
country: Sweden
facility: Karolinska Institutet/Karolinska University Hospital
Study officials:
References: