Clinical trial NCT03746145: A Counter Measure for the Effects of Immune and Microbiome Changes in Environments With Limited ANtigen Diversity (ICELAND-TWO)
Official title: A Counter Measure for the Effects of Immune and Microbiome Changes in Environments With Limited ANtigen Diversity (ICELAND-TWO) - a Randomized Double Blind Controlled Pilot Study With Bifidobacterium Longum 1714
Study type: INTERVENTIONAL
Overall status: UNKNOWN
Phase: NA
Conditions: Confinement and Isolation
Keywords: probiotics
microbiota
gastrointestinal
immune system
immunology
stress
Bifidobacterium
hypoxia
confinement
isolation
Lead sponsor: University Hospital Tuebingen
Sponsor class: OTHER
Collaborators: name: Dr. Isabelle Mack, Co-PI, University Hospital Tübigen
name: Dr. Claude Lambert, University Hospital of Saint-Etienne
name: Dr. John Penders, Maastricht University Medical Center
name: Dr. Joël Doré, Institut National de la Recherche Agronomique
name: European Space Agency
First posted: 2018-11-19
Enrollment: 40
Sex: ALL
Minimum age (years): 18
Maximum age (years): 99
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: PARALLEL
Intervention model description: During the entire winter over (WO) period, from arrival at COCORDIA until departure (1 year), participants receive 2g nutritional supplement powder (sachet, containing 10e11 CFU Bifidobacterium longum 1714 or placebo) once every day, to be taken in the morning with breakfast throughout the year.
Primary purpose: BASIC_SCIENCE

Brief summary: There is evidence to indicate that the gut microbiota has an effect on mental well-being and stress behaviours. This is highly relevant to both life on earth and spaceflight missions, in which mental well-being is crucial to mission success. Bifidobacterium longum 1714 (BL 1714), will be tested in the project ICELAND-2 in a double-blind, randomized placebo-controlled pilot study in crew members of the Antarctica station CONCORDIA in order to test stress resilience and possible outcomes on the immune system since the metabolic stress response and the immune system are closely interrelated. Crew members of CONCORDIA station have a prolonged stay (roughly 12 months) in a confined (antigen exposure restricted, overclean) and isolated environment, mimicking the situation of long-term space travel. ICELAND-2 will be conducted over three consecutive winter-over periods (3 years).

The project ICELAND-2:

1. evaluates the effect of the probiotic BL1714 on well-being regarding mood, social integration and stress and other parameters .
2. determines the effect of an antigen-limited environment like Concordia on gut microbiota, immune system and epigenetics.
3. examines the interaction between the factors mentioned in 1.
4. examines the role of nutrition intake and behaviour in the interplay mentioned above.

Detailed description: EXPERIMENT CONCEPT

This is a double-bind, controlled pilot study where volunteers will receive a novel nutritional supplement powder (sachet, containing 1011 CFU Bifidobacterium longum 1714 (Alimentary 11 Health, Cork, Ireland) or placebo) once every day, to be taken in the morning with breakfastthroughout the year. The intervention starts for every participant at arrival (individual start). Using this study design, benefits of the probiotic on height adaptation can be also studied.

The experimental concept is based on the collection of biosamples (saliva, blood, stool) and some paper-pencil and documentary data only. Thereby, the interference of the project with the daily activities of the CONCORDIA crew is minimal. Both, female and male participants are included.

1. One blood, one saliva and one stool sample prior to the CONCORDIA stay, in addition to paper-pencil data collection once at the same time (-2 to 3 months prior to the stay - T Zero, BDC)
2. Three blood as well as a saliva samples (month 1, months 4 and 9, corresponding to T0, T4 and T9) during the CONCORDIA stay, as well as monthly collection of stool samples (months 1 to 9, T1 to T9), plus questionnaire data (paper-pencil data and/or online).
3. One blood, one saliva and one stool sample after the CONCORDIA stay, in addition to paper-pencil data collection once at the same time (3 to 6 months after the stay - T12)

Saliva Sampling: a standard saliva sampling kit (Oragene-DNA - OG-500, DNA Genotec, Ottawa, ON, Canada) will be used (see Figure 2).

Stool Sampling: a standard stool sampling kit (Sarstedt) will be used (see Figure 3) to retrieve 3 small samples from each stool, and stored in separated containers.

Feces Catcher: Paper-based stool collection aid (with instruction imprinted in variable language (FecesCatcher®) (Figure 4)

Blood sampling: Blood collection will be performed in two 5 ml heparinized tubes (total 10 ml for cell collection) and one dried tube of 5 ml per blood sampling. An adequate number of separation tubes and cryotubes for processing of blood will also be provided.

PROCEDURE OUTLINE

Saliva sampling: can be done at any time prior to the CONCORDIA stay, easiest during the pre-departure crew meeting. Saliva sampling will be repeated at month 4 and 9 as well as post CONCORDIA stay. Saliva samples do not need any preprocessing and can be stored at room temperature for the remaining time.

During the pre-departure meeting, a 1-hour interview related to nutritional habits (including gastrointestinal symptoms like bowel movements) should be done. Paper-pencil tests (1 hour) will be collected as well. This will include the Food Frequency Questionnaire (FFQ), the gastrointestinal symptom rating scale (GSRS) and a structured questionnaire related to previous illnesses, drug intake and medical treatments. The FFQ and the GSRS will be assessed in Concordia at the same times as the blood sampling is performed.

Stool sampling and short well-being assessment: Sampling (three aliquots, pea-size, in separate containers) can be done at the discretion of each participating CONCORDIA crew member once every month, but preferentially at approximately same day of the months each time. No coordinated sampling between crew members is needed, but the day should be noted on the sampling kits/diary. This should be supervised and documented by the ESA MD. Along with the stool sampling the participants will be asked to rate on a visual analogue scale mood, stress and social integrity. Additionally, the ESA MD will provide the information for every participant. This measure is needed as outcome for the RCT.

Food records: The ESA MD is asked to take pictures of meals/food/food condition on a regular base if possible. Most importantly, the MD should take standardized photos of the meals (of the last three days prior to the and the days of the stool sampling. The camera and storage devices have been handed out to the MD in Aosta, Italy.

Alternatively and/or in addition the cook will record his cooking activities.

T - 3 days before stool sampling:

ESA MD takes photo (with a reference 2 euro coin) of breakfast meal, lunch meal and dinner meal

T - 2 days before stool sampling:

ESA MD takes photo (with a reference 2 euro coin) of breakfast meal, lunch meal and dinner meal

T - 1 days before stool sampling:

ESA MD takes photo (with a reference 2 euro coin) of breakfast meal, lunch meal and dinner meal

Days of stool sampling:

ESA MD takes photo (with a reference 2 euro coin) of breakfast meal, lunch meal and dinner meal

Pre-processing of stool samples is not needed, samples are stored away at -50°C.

Blood sampling: Blood withdrawal for immune testing must be performed by a single phlebotomy, and can be performed at the time of programmed health checks of the CONCORDIA staff. Blood collection must be performed in two 5 ml heparinized tubes (total 10 ml for cell collection), and one dried tube of 5 ml for serum.

Pre-processing of the Blood Sample (to be performed by the ESA investigator physician)

For cognate immune functions, live cells are needed and must be frozen and stored in preserving conditions so they can be resuscitated after that. This means rapid processing after collection (within 4-6 hours), fast freezing and in preserving medium (with DMSO) below -40°C.

Blood Cell storage: Vacutainer® CPT™ Cell Preparation tubes with Sodium Heparin are provided to simplify cell preparation (Figure 5, left). CPT™ tubes directly contain ficoll (density buffer) and a gel ready for centrifugation. The blood is collected on the top of the gel like usual vacutainer tubes. Centrifugation will separate red blood cells, granulocytes (going below the gel) from mononuclear cells and plasma staying on the top. Mononuclear cells need washing in Sterile Hanks buffer (provided) and the pellet re-suspended in freezing buffer (provided) and directly frozen at least below 40°C.

Eligibility criteria: Inclusion Criteria:

Crew members of the Concordia station in Antarctica

Exclusion Criteria:

Participants which are not crew members of the Concordia station in Antarctica

Primary outcomes:
measure: Expert rating by medical doctor on well-being of participant
timeFrame: up to 12 months
Secondary outcomes:
measure: Participant rating on well-being
timeFrame: measurement monthly over 12 months
measure: Discrepancy between ratings on well-being
timeFrame: measurement monthly over 12 months
measure: Gastrointestinal symptoms
timeFrame: Measurements at 1 day predeparture, in Antarctica at months 4,9 and 12 and 6 months postdeparture
measure: Gastrointestinal microbiota
timeFrame: stool samples collected at 1 day predeparture, in Antarctica monthly over 1 year and 6 months postdeparture
measure: Immune system
timeFrame: Measurements at 1 day predeparture, in Antarctica at months 4,9 and 12 and 6 months postdeparture
measure: Epigenetics
timeFrame: Measurements at 1 day predeparture, in Antarctica at months 4,9 and 12 and 6 months postdeparture
measure: Interaction between microbiota and immune biology (including epigenetics)
timeFrame: Measurements at 1 day predeparture, in Antarctica at months 4,9 and 12 and 6 months postdeparture
measure: Nutrition
timeFrame: FFQ: Measurements at 1 day predeparture, in Antarctica at months 4,9 and 12 and 6 months postdeparture
measure: Medical and psychological data records assessed by the medical doctors of the European Space Agency
timeFrame: 1 day Predeparture, Antarctica winterover of 12 months
measure: POMS
timeFrame: 1 day Predeparture, Antarctica winterover of 12 months
measure: PANAS
timeFrame: 1 day Predeparture, Antarctica winterover of 12 months
Other outcomes:
Locations:
city: Concordia
country: Antarctica
facility: Concordia Station
Study officials:
References: