Clinical trial NCT03278145: Dependence Receptors and Leukemia
Official title: Dependence Receptors in Childhood Acute Leukemia
Study type: OBSERVATIONAL
Overall status: UNKNOWN
Conditions: Acute Lymphoblastic Leukemia
Acute Leukemia
Lead sponsor: Hospices Civils de Lyon
Sponsor class: OTHER
First posted: 2017-09-11
Enrollment: 30
Sex: ALL
Minimum age (years): 1
Maximum age (years): 18
Standard ages: CHILD
ADULT
Observational model: COHORT
Time perspective: PROSPECTIVE
Sampling method: NON_PROBABILITY_SAMPLE
Study population: The target population consists of patients aged 1 to 18 years treated at the Institute of Hematology and Pediatric Oncology (IHOPe), a department of Prof. Y. Bertrand, for acute leukemia (LA) at the time of diagnosis initial, or relapse, after obtaining signed parental consent. The same patients will benefit from a new sample at the time of their remission balance.

Brief summary: Acute leukaemias (AL) are the first cause of cancer in children, with a majority of B acute lymphoblastic leukemia (ALL). Some of the processes causing leukemogenesis are already identified and well characterized in some AL subtypes such as translocation t (12; 21) of good prognosis in ALL. However, translocations are not sufficient to explain all the different processes of leukemogenesis, and other processes such as genetic / epigenetic mutations leading to oncogene activation / inhibition of tumor suppressor genes are the object research. Among the latter, mutations in tumor suppressor genes such as DCC (Deleted in Colorectal Cancer) have recently been identified in solid cancers, such as in hemopathies. This gene was subsequently characterized as encoding a "dependence receptor" specifically binding to its Netrin-1 ligand.

Dependence receptors (RDs) are transmembrane receptors that cause cell death in the absence of their ligand. RD decreases tumor progression and overexpression of their ligands is observed in many cancers, such as B lymphomatous hemopathies in adults. Inhibition of the RD-ligand interaction constitutes a new and original therapeutic target in oncology.

The aim of this study is to investigate whether RDs, in particular DCC, are expressed in acute leukemia cells at the time of diagnosis or relapse in patients aged 1 to 18 years, and then in these patients at the time of the remission balance. This research will be both qualitative and quantitative.


Eligibility criteria: Inclusion Criteria:

* aged between 1 and 18 years
* taken care of at the Institute of Hematology and Pediatric Oncology (Service of Professor Yves Bertrand, IHOPe)
* for acute lymphoblastic or myeloblastic leukemia
* initial diagnosis or relapse
* who do not have a vital emergency criterion at the time of taking care (see exclusion criteria)
* affiliated to a social security scheme (100% assumed)
* after signing the informed consent of the holders of parental authority

Exclusion Criteria:

* less than 1 year, or more than 18 years to diagnosis
* with chronic leukemia
* severe anemia at diagnosis (hemoglobin \<40g / l), or a state of shock whatever the cause (infectious, cardiogenic, hypovolemic)

Primary outcomes:
measure: Detection of specific labeling of the DCC-dependent receptor on the surface of leukemic cells
timeFrame: Maximum 4 months (sampling at the time of diagnosis / relapse and remission)
Secondary outcomes:
Other outcomes:
Locations:
city: Lyon
country: France
facility: Institut d'Hématologie et d'Oncologie Pédiatrique
Study officials:
References: