Clinical trial NCT06264193: The Impact of Hypoxia on the Biochemical and Morphological Parameters of Blood in Rowers.
Official title: The Effects of Live High-train Low on EPO, VEGF, and Hematological Variables in Elite Rowers
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Hypoxia
Keywords: hypoxia
rowers
exercise
EPO
athletes
LH-TL
Lead sponsor: Poznan University of Physical Education
Sponsor class: OTHER
First posted: 2024-02-16
Enrollment: 13
Sex: MALE
Standard ages: CHILD
ADULT
OLDER_ADULT
Accepts healthy volunteers: yes
Allocation: NA
Intervention model: SINGLE_GROUP
Intervention model description: The hypoxic group included rowers participating in sports training and the LH-TL method, hypoxic rooms (FiO2=14.5%, corresponds to an altitude of 3000 meters). And the control group participating only in sports training and living in normoxic rooms, at the same sports camp.
Primary purpose: DIAGNOSTIC

Brief summary: The response to hypoxia is very individual and epending on many aspects, such as the type of training, duration, intensity, or hypoxic stimulus, hypoxia affects the athlete in various ways. The results of this study have shown that 18 days of the LH-TL method does not significantly increase the level of EPO and VEGF in rowers. However, reticulocytes, immature red blood cells, have shown significant differences after 18-d LH-TL between groups. Further research should be carried out to investigate an optimal hypoxic dose and time, which will raise EPO, VEGF, and morphology variables.

Detailed description: The Polish national rower team athletes, 13 males (ages 27,0±4,2 years), were randomly divided into two groups: 1) The hypoxic group (H) included 8 rowers participating in sports training and the LH-TL method, hypoxic rooms (FiO2=14.5%, corresponds to an altitude of 3000 meters). 2) The control group (C) presented 5 rowers participating only in sports training and living in normoxic rooms, at the same sports camp. Rowers were observed during the preparatory period. The hypoxic group lived and slept in hypoxic rooms, for at least 8-14 hours per day. During the camp, all rowers followed the same training schedule and diet.All participants' saturation and heart rate were measured daily, morning and evening, throughout the camp period. Venous blood samples were taken four times, at the beginning of the camp (Baseline), after 5 days, after 12 days, and after 18 days of the camp. Body composition parameters such as body mass index, body fat, and free fat mass were estimated using the bioelectrical impedance method of an analyzer Tanita BC 418 (Japan). Data analysis was performed using Statistica. The response to hypoxia is very individual. Depending on many aspects, such as the type of training, duration, intensity, or hypoxic stimulus, hypoxia affects the athlete in various ways. The results of this study have shown that the blood parameters did not increase significantly in the hypoxic group compared to the control group. However, reticulocytes, immature red blood cells, have shown significant differences after 18-d LH-TL between groups. Further research should be carried out to investigate an optimal hypoxic dose and time, which will raise EPO, VEGF, and morphology variables.

Eligibility criteria: Inclusion Criteria:

* member of national rower's teams, and sports camp participant.

Exclusion Criteria:

* negative symptoms of hypoxia, and/or injury.

Primary outcomes:
measure: Erythropoietin (EPO) mlU/ml
timeFrame: At the beginning of the camp (Baseline), after 5 days (T1), after 12 days (T2), and after 18 days (T3) of the camp.
measure: vascular endothelial growth factor (VEGF) mlU/ml
timeFrame: At the beginning of the camp (Baseline), after 5 days (T1), after 12 days (T2), and after 18 days (T3) of the camp.
Secondary outcomes:
measure: creatine kinase (CK) ng/ml
timeFrame: At the beginning of the camp (Baseline), after 5 days (T1), after 12 days (T2), and after 18 days (T3) of the camp.
measure: Hs C-Reactive Protein (hsCRP) mg/L
timeFrame: At the beginning of the camp (Baseline), after 5 days (T1), after 12 days (T2), and after 18 days (T3) of the camp.
measure: hemoglobin (Hb)g/dL
timeFrame: At the beginning of the camp (Baseline), after 5 days (T1), after 12 days (T2), and after 18 days (T3) of the camp.
measure: hematocrit (Htc)%
timeFrame: At the beginning of the camp (Baseline), after 5 days (T1), after 12 days (T2), and after 18 days (T3) of the camp.
measure: red blood cells (RBC)mln/mm3
timeFrame: At the beginning of the camp (Baseline), after 5 days (T1), after 12 days (T2), and after 18 days (T3) of the camp.
measure: white blood cells (WBC)10 3/µL
timeFrame: At the beginning of the camp (Baseline), after 5 days (T1), after 12 days (T2), and after 18 days (T3) of the camp.
measure: reticulocytes (Ret)‰
timeFrame: At the beginning of the camp (Baseline), after 5 days (T1), after 12 days (T2), and after 18 days (T3) of the camp.
measure: SpO2 - oxygen saturation (%)
timeFrame: every day, morning and evening, while sports camp
measure: bpm - beats per minute(%)
timeFrame: every day, morning and evening, while sports camp
Other outcomes:
Locations:
Study officials:
affiliation: Poznań University of Physical Education, Faculty of Sport Sciences in Gorzów Wielkopolski, Poland;
name: Anna Kasperska
role: PRINCIPAL_INVESTIGATOR
References:
citation: Kasperska A, Zembron-Lacny A. The effect of intermittent hypoxic exposure on erythropoietic response and hematological variables in elite athletes. Physiol Res. 2020 Apr 30;69(2):283-290. doi: 10.33549/physiolres.934316. Epub 2020 Mar 23.
citation: Wilber RL. Application of altitude/hypoxic training by elite athletes. Med Sci Sports Exerc. 2007 Sep;39(9):1610-24. doi: 10.1249/mss.0b013e3180de49e6.
citation: Czuba M, Fidos-Czuba O, Ploszczyca K, Zajac A, Langfort J. Comparison of the effect of intermittent hypoxic training vs. the live high, train low strategy on aerobic capacity and sports performance in cyclists in normoxia. Biol Sport. 2018 Mar;35(1):39-48. doi: 10.5114/biolsport.2018.70750. Epub 2017 Oct 11.
citation: Ploszczyca K, Langfort J, Czuba M. The Effects of Altitude Training on Erythropoietic Response and Hematological Variables in Adult Athletes: A Narrative Review. Front Physiol. 2018 Apr 11;9:375. doi: 10.3389/fphys.2018.00375. eCollection 2018.