Clinical trial NCT06130683: Single-Cell Sequence Technology Used to Reveal Heterogeneity of Secondary Hyperparathyroidism
Official title: Single-Cell Sequence Technology Used to Reveal Heterogeneity of Secondary Hyperparathyroidism
Study type: OBSERVATIONAL
Overall status: ACTIVE_NOT_RECRUITING
Conditions: Secondary Hyperparathyroidism
Lead sponsor: China-Japan Friendship Hospital
Sponsor class: OTHER
First posted: 2023-11-14
Enrollment: 12
Sex: ALL
Minimum age (years): 18
Maximum age (years): 80
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: no
Observational model: OTHER
Time perspective: PROSPECTIVE
Sampling method: NON_PROBABILITY_SAMPLE
Study population: People who undertake surgery for secondary hyperparathyroidism or primary hyperparathyroidism and other neck surgeries in our China-Japan Friendship Hospital

Brief summary: This project intends to select cases that meet the research requirements, take secondary hyperparathyroidism, primary hyperparathyroidism and normal human parathyroid tissue, a total of three groups, 4 cases in each group, through the method of single-cell transcription and sequencing, construct a map of human parathyroid function types, reveal the gene structure and gene expression status of cells, and visualize the expression characteristics, intercellular heterogeneity, and heterogeneity of cell subsets of secondary hyperparathyroid cells in a hierarchical manner, draw a single-cell map, and compare the differences between groups. To explore the pathogenesis of secondary hyperparathyroidism.

Secondary hyperparathyroidism, parathyroid tissue of primary hyperparathyroidism and normal parathyroid tissue obtained by accident were collected, frozen and preserved, frozen tissue thawed, single-cell suspension was prepared and each cell was specifically labeled by the Mozhuo Genomics system, after oil breaking, polymerase chain reaction amplification, reverse transcription to obtain complementary DNA, and a library of complementary DNA that passed quality inspection was constructed to obtain high-quality data of parathyroid cells. Cell Ranger, R Seurat package, and t-SNE dimensionality reduction diagram were used to reduce the dimensionality, cluster, and visualize the data.

In order to construct a single-cell atlas of parathyroid glands, investigators performed cluster analysis of similar cells according to the gene expression profile, and then visualized the data by t-SNE. According to the results of cell clustering, the specific and highly expressed genes in each cell cluster were identified. Cell populations were identified according to the expression of landmark genes, and the differences in cell types and proportions between groups were compared.


Eligibility criteria: Inclusion Criteria:

* Study participants with a diagnosis of secondary hyperparathyroidism who underwent surgical treatment
* Study participants with a diagnosis of primary hyperparathyroidism who underwent surgical treatment
* Study participants who have obtained informed consent

Exclusion Criteria:

* Other non-secondary hyperparathyroidism conditions such as primary hyperparathyroidism were excluded at the time of inclusion of study participants with essential hyperparathyroidism.
* Other non-primary hyperparathyroid conditions such as secondary hyperparathyroidism were excluded at the time of inclusion of study participants with essential hyperparathyroidism.
* Refusal of informed consent.

Primary outcomes:
measure: Construct the single-cell atlas
timeFrame: One year
Secondary outcomes:
Other outcomes:
Locations:
city: Beijing
country: China
facility: China and Japan Friendship Hospital
Study officials:
affiliation: China-Japan Friendship Hospital
name: Meng Yang
role: PRINCIPAL_INVESTIGATOR
References: