Clinical trial NCT04805359: Effect of Hypoxia Exercise on Erythrocyte Aggregability
Official title: Effect of Hypoxia Exercise on Erythrocyte Aggregability
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Hypoxia
Training
Keywords: Erythrocyte
Aggregation
Lead sponsor: Chang Gung Memorial Hospital
Sponsor class: OTHER
First posted: 2021-03-18
Enrollment: 60
Sex: MALE
Minimum age (years): 20
Maximum age (years): 30
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: PARALLEL
Primary purpose: TREATMENT

Brief summary: Pathological erythrocyte aggregation reduces capillary perfusion and oxygen transfer to tissue. The aggregation is determined by opposing forces, the repulsive force between cells, cell-to-cell adhesion induced by plasma fibrinogen, and the disaggregating shear force generated by blood flow. The investigators investigate how hypoxic exercise affects intrinsic/extrinsic factors of aggregation. Sixty sedentary males were randomly assigned into either hypoxic (HE; FiO2=15%, n=20) or normoxic (NE; FiO2=21%, n=20) training groups for 30 min·d-1, 5 d·wk-1 for 6 weeks at 60% of maximum work rate or to a control group (CTL; n=20). A hypoxia exercise test (HET, FiO2=12%) was performed before and after the intervention. The erythrocyte aggregation, binding affinity of fibrinogen and membrane biomarkers were determined by an ektacytometry and flow cytometry, respectively.

Detailed description: The aim of this study is to clarify the effects of exercise training with and without hypoxia on the erythrocyte aggregation, further providing a feasible strategy for developing an appropriate exercise regimen that improves cardiopulmonary fitness and minimizes the risk of hemorheological disorders. This study evaluated the distinct effects of hypoxic exercise training (HE) and normaxia exercise training (NE) for 6 weeks on the following issue: (1) intrinsic/extrinsic erythrocyte aggregation, (2) binding affinity of fibrinogen to erythrocyte, (3) erythrocyte turnover rate and (4) characteristic of mature erythrocytes in blood, under hypoxic exercise test (HET) (100W under air 12% O2 condition) in healthy sedentary men.

Eligibility criteria: Inclusion Criteria:

* Having a sedentary lifestyle (without regular exercise, exercise frequency ≤ once weekly, duration \< 20 min).

Exclusion Criteria:

* Exposed to high altitudes (\> 3000 m) for at least 1 year.
* Smoker
* Taking medications or vitamins
* Having any cardiopulmonary/hematological risk.

Primary outcomes:
measure: Total Erythrocyte Aggregation
timeFrame: 8 weeks
Secondary outcomes:
measure: Cardiopulmonary capacity
timeFrame: 8 weeks
measure: The affinity between erythrocyte and fibrinogen
timeFrame: 8 weeks
measure: Senescence-Related Molecules on Erythrocyte
timeFrame: 8 weeks
measure: Adhesion-Related Molecules on Erythrocyte
timeFrame: 8 weeks
measure: The influences of fibrinogen to erythrocytes
timeFrame: 8 weeks
measure: The influences of the intrinsic factor to erythrocytes
timeFrame: 8 weeks
measure: The influences of the extrinsic factor to erythrocytes
timeFrame: 8 weeks
Other outcomes:
Locations:
Study officials:
affiliation: Chang Gung Memorial Hospital
name: Jong-Shyan Wang, PhD
role: PRINCIPAL_INVESTIGATOR
References: