Clinical trial NCT04865198: Neuralized1 and RGS14 Genes
Official title: Role of Neuralized1 and RGS14 Genes With ASD Patients
Study type: OBSERVATIONAL
Overall status: COMPLETED
Conditions: Autism Spectrum Disorder
High-functioning Autism
Healthy
Keywords: ASD
High Functioning Autism
NEURL1
RGS14
Gene expression
Lead sponsor: TC Erciyes University
Sponsor class: OTHER
First posted: 2021-04-29
Enrollment: 60
Sex: ALL
Minimum age (years): 2
Maximum age (years): 16
Standard ages: CHILD
Accepts healthy volunteers: yes
Observational model: OTHER
Time perspective: CROSS_SECTIONAL
Sampling method: PROBABILITY_SAMPLE
Study population: Group 1: Patients diagnosed with ASD in a child psychiatry clinic. Group 2: Patients diagnosed with HFA in a child psychiatry clinic. Group 3: Healty controls

Brief summary: Autism is a broad spectrum neurodevelopmental disease. Some individuals with ADS by high cognitive functions are diagnosed with High Functioning Autism (HFA). In some studies, it has been shown that NEURL1 gene increases learning and memory and RGS14 gene is suppressed them. We aimed to evaluate the differences between the expression levels of these genes between ASD, HFA and healthy controls and the role of these genes in the pathogenesis of ASD. Patients with 20 ASD and 20 HFA, and 20 healthy controls compatible with patient ages were included in this study. Expression of NEURL-1 and RGS14 genes was evaluated by quantitative Real Time PCR (qRT-PCR).

Detailed description: ASD is a neurological disease starting in the early stages of life and is characterized by cognitive and behavioral disorders (Ansel et al., 2008;Alvares et al., 2020). It is considered that the etiology of ASD stems from genetic, epigenetic and environmental factors; however, it has not yet been definitively clarified(Ito et al., 2017).

we aimed to evaluate the differences between the expression levels of these genes between ASD, HFA and healthy controls and the role of these genes in the pathogenesis of ASD.

Method:

Patients with ASD (n=20) and HFA (n=20), and healthy controls (n=20) compatible with patient ages were included in this study. Clinical evaluations of the patients were made and classification was made in accordance with DSM-IV diagnostic criteria.

High Pure RNA Isolation Kit (Roche Diagnostic, Version 12, Germany) was used for RNA isolation. cDNA synthesis was performed from these RNAs with the ranscriptor High Fidelity cDNA Synthesis Kit (Roche Diagnostics, GmbH, Mannheim).

qRT-PCR was performed using the LightCycler®480 Real Time Ready Assay Master Probe Kit (Roche Diagnostics, GmbH, Mannheim).The incubation was made with the PCR device program for 10 minutes at 95oC for 45 cycles, for 10 sec at 95oC, and for 60 sec at 60oC. The Ct values were obtained from the Light Cycler 480 Software Program, and both genes were analyzed separately. The comparative CT method (2-ΔΔCT) was used to determine the relative quantification of target genes, normalized to a housekeeping gene (β-actin).

Statisticaly:

The results of the experiments were evaluated using R 3.1.1 (www.r-project.org). and Chi-Square Tests, Mann-Whitney U-Test, Kruskal-Wallis H-Tests. The P\<0.05 level was taken as significant.

Eligibility criteria: Inclusion Criteria:

* Being diagnosed ASD or HFA patient,
* Being between the ages of 2-16.

Exclusion Criteria:

* To use medicine,
* Have a other syndromic illness,
* Being younger than 2 years old or over 16 years old.

Primary outcomes:
measure: NEURL1 gene expression levels
timeFrame: Two months
measure: RGS14 gene expression levels
timeFrame: Two months
Secondary outcomes:
measure: Age
timeFrame: an average of 1 year
measure: Gender
timeFrame: an average of 1 year
measure: Intellectual disability (ID)
timeFrame: an average of 1 year
measure: Consanguinity
timeFrame: an average of 1 year
measure: Presence of Neurological Disease in Relatives
timeFrame: an average of 1 year
measure: Corelation tests
timeFrame: an average of 1 year"
Other outcomes:
Locations:
Study officials:
affiliation: Alanya Alaaddin Keykubat University
name: Hamiyet Eciroğlu, Phd. St.
role: PRINCIPAL_INVESTIGATOR
affiliation: TC Erciyes University
name: Elif F. Şener, Assoc. Prof.
role: PRINCIPAL_INVESTIGATOR
affiliation: Ankara University
name: Didem B. Öztop, Assoc. Prof.
role: PRINCIPAL_INVESTIGATOR
affiliation: TC Erciyes University
name: Sevgi Özmen, Assoc. Prof.
role: PRINCIPAL_INVESTIGATOR
affiliation: TC Erciyes University
name: Dilek Kaan, Phd.
role: PRINCIPAL_INVESTIGATOR
affiliation: TC Erciyes University
name: Yusuf Özkul, Prof. Dr.
role: STUDY_CHAIR
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