Clinical trial NCT03879187: Molecular and Hormonal Responses to Diet-Induced Insulin Resistance
Official title: Molecular and Hormonal Responses to Diet-Induced Insulin Resistance
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Healthy
Keywords: High-fat diet
Insulin
Glucose
Muscle
eNOS
Isotopes
Lead sponsor: Loughborough University
Sponsor class: OTHER
First posted: 2019-03-18
Enrollment: 15
Sex: ALL
Minimum age (years): 18
Maximum age (years): 40
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: NA
Intervention model: SINGLE_GROUP
Intervention model description: 15 healthy individuals were assessed before and after consuming a high-fat, high-energy diet for 7 days.
Primary purpose: BASIC_SCIENCE

Brief summary: This study determined the effect of 7 days of high-fat overfeeding on whole-body glycaemic control, glucose kinetics, skeletal muscle insulin signalling, and markers of skeletal muscle microvascular function in 15 healthy young individuals.

Detailed description: Fifteen healthy, young individuals underwent metabolic testing before and after 7 days of high-fat overfeeding. We determined whole-body glycaemic control, glucose kinetics, skeletal muscle insulin signalling, and markers of skeletal muscle microvascular function. Stable isotope tracers were used to assess the rate of appearance of glucose from the gut, liver glucose output and whole-body glucose disposal. Insulin signalling was determined by Western blotting of skeletal muscle tissue. Microvascular function was assessed through the use of quantitative immunofluorescence microscopy. These measurements were performed both postabsorptively and following the ingestion of 50 grams of glucose + 15 grams of protein.

Eligibility criteria: Inclusion Criteria:

* Aged between 18 and 40 years
* Physically active
* Non-smoker
* Not taking any medication known to interfere with the study
* No history of cardiovascular disease or diabetes
* Weight stable for the last 6 months

Exclusion Criteria:

* Outside of age range
* Sedentary
* Smoker
* Taking medication known to interfere with the study
* History of cardiovascular disease or diabetes
* Unstable weight within last 6 months

Primary outcomes:
measure: Change in glucose concentration
timeFrame: Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart)
measure: Change in insulin concentration
timeFrame: Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart)
measure: Change in glucose kinetics (i.e., rate of appearance and disappearance) assessed using isotope tracers
timeFrame: Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart)
measure: Change in skeletal muscle insulin signalling
timeFrame: Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart)
measure: Change in markers of microvascular function
timeFrame: Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart)
Secondary outcomes:
measure: Change intramuscular triglyceride content
timeFrame: Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart)
Other outcomes:
Locations:
Study officials:
References:
citation: Parry SA, Turner MC, Woods RM, James LJ, Ferguson RA, Cocks M, Whytock KL, Strauss JA, Shepherd SO, Wagenmakers AJM, van Hall G, Hulston CJ. High-Fat Overfeeding Impairs Peripheral Glucose Metabolism and Muscle Microvascular eNOS Ser1177 Phosphorylation. J Clin Endocrinol Metab. 2020 Jan 1;105(1):dgz018. doi: 10.1210/clinem/dgz018.