Clinical trial NCT02467283: Gene Expression Profiles in Chronic Periodontitis
Official title: Gene Expression Profiles in Chronic Periodontitis: A Gene Network-based Microarray Analysis
Study type: OBSERVATIONAL
Overall status: COMPLETED
Conditions: Chronic Periodontitis
Keywords: chronic periodontitis
genetics
microarray analysis
gingiva
genes
inflammation
Lead sponsor: Kocaeli University
Sponsor class: OTHER
First posted: 2015-06-10
Enrollment: 46
Sex: ALL
Minimum age (years): 18
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: yes
Observational model: CASE_CONTROL
Time perspective: CROSS_SECTIONAL
Sampling method: PROBABILITY_SAMPLE
Study population: The patientswere recruited from those patients seeking dental treatment in Kocaeli University, Faculty of Dentistry Periodontology Clinics.

Brief summary: Current concepts of the causes of periodontitis implicate a bacterial infection as the primary cause of the disease. Several bacterial species residing in a biofilm on tooth surfaces are closely associated with periodontitis. Chronic periodontitis (CP) is initiated and sustained by bacterial plaque, but host defense mechanisms play an integral role in its pathogenesis.

Gene expression profiling is a powerful means of generating comprehensive genome-level data sets on diseases such as cancer, asthma, rheumatoid disorders and periodontitis, and provides significant insight information for these diseases. Gene expression profiling may provide an evidence for involving genes in the pathogenesis of CP and generate further information other than clinical signs and symptoms of CP.

The aim of this study was to identify gene-expression patterns of patients with chronic periodontitis by whole-transcriptome gene-expression analyses.


Eligibility criteria: Inclusion Criteria:

1. CP patients had at least 20 teeth exhibiting \>30% of measured sites with \>5mm clinical attachment loss.
2. Had bleeding on probing (BOP) at \>50% of the proximal sites.

Exclusion Criteria:

1. Had any known systemic diseases that can influence the periodontal status, oral diseases other than chronic periodontitis and ongoing orthodontic therapy.
2. History of hepatitis, HIV infection, or diabetes; requirement for antibiotic prophylaxis, pregnancy or lactation; and long-term use of anti-inflammatory drugs.
3. Should be free of systemic diseases
4. Individuals had to be older than 18 years of age.

Primary outcomes:
measure: mRNA quantification
timeFrame: 2 months
Secondary outcomes:
measure: Gen networking map
timeFrame: 2 months
Other outcomes:
Locations:
Study officials:
affiliation: Chair and Associate Professor of Dept. of Periodontology
name: Esra Guzeldemir-Akcakanat, DDS, PhD
role: PRINCIPAL_INVESTIGATOR
References:
citation: Armitage GC. Development of a classification system for periodontal diseases and conditions. Ann Periodontol. 1999 Dec;4(1):1-6. doi: 10.1902/annals.1999.4.1.1.
citation: Demmer RT, Behle JH, Wolf DL, Handfield M, Kebschull M, Celenti R, Pavlidis P, Papapanou PN. Transcriptomes in healthy and diseased gingival tissues. J Periodontol. 2008 Nov;79(11):2112-24. doi: 10.1902/jop.2008.080139.
citation: Savli H, Sunnetci D, Cine N, Gluzman DF, Zavelevich MP, Sklyarenko LM, Nadgornaya VA, Koval SV. Gene expression profiling of B-CLL in Ukrainian patients in post-Chernobyl period. Exp Oncol. 2012;34(1):57-63.