Clinical trial NCT03151954: The Activation of Hippo Pathway in Nasal Polyps
Official title: Yap, the Nuclear Target of Hippo Signaling, Regulates Nasal Epithelium Stem Cells Aberrant Proliferation and Differentiation in Nasal Polyps
Study type: INTERVENTIONAL
Overall status: UNKNOWN
Phase: NA
Conditions: Nasal Polyps
Keywords: nasal epithelium stem cells
Hippo-YAP pathway
proliferation
differentiation
Lead sponsor: Third Affiliated Hospital, Sun Yat-Sen University
Sponsor class: OTHER
Collaborators: name: National University of Singapore
First posted: 2017-05-12
Enrollment: 100
Sex: ALL
Minimum age (years): 18
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: no
Allocation: NON_RANDOMIZED
Intervention model: PARALLEL
Primary purpose: TREATMENT

Brief summary: Hippo-YAP pathway is a newly discovered pathway controlling the growth, and plays important roles in the regulation of stem cell function and tissue regeneration. Previously we found that a higher expression of YAP in NP, and LPS was able to activate the hippo-YAP pathway in NESCs. Thus, we hypothesize that the aberrant proliferation and differentiation of NESCs could be regulated via the hippo-YAP pathway.

Detailed description: Studies have shown that the development of nasal polyps (NP) are closely related to the epithelial remodeling.The investigators have shown that nasal epithelial stem cells(NESCs) derived from NP had reduced growth and proliferation dynamics in vitro culture,and differentiated into ciliated cells with impaired cilia architecture and ciliogenesis.Further studies demonstrated that NESCs were involved in the aberrant damage repair process of the epithelial remodeling in NP.Hippo-YAP pathway is a newly discovered pathway controlling the growth,and plays important roles in the regulation of stem cell function and tissue regeneration.Previously the investigators found that a higher expression of YAP in NP,and LPS was able to activate the hippo-YAP pathway in NESCs.Thus,the investigators hypothesize that the aberrant proliferation and differentiation of NESCs could be regulated via the hippo-YAP pathway,which may result in the epithelial remodeling.In this study,the investigators intend to investigate the mechanism of the hippo-YAP pathway in regulating aberrant proliferation and differentiation of NESCs and explore its upstream signaling and downstream target genes,using the NESCs gas-liquid interface culture system,and a silencing and overexpression of the hippo-YAP pathway component strategy.The investigators aim to unravel the effect and mechanism of the hippo-YAP pathway in the epithelial remodeling of NP,providing new targets and strategy for the improvement or reversal of NP epithelial remodeling.

Eligibility criteria: Inclusion Criteria:

* Clinical diagnosis of nasal polyps or deviation of nasal septum based on CT
* Must be able to withstand surgery
* At least 18 years old
* A sufficient level of education to understand study procedures and be able to communicate with site personnel and adhere to the follow-up;
* Accepted informed consent verbally and in writing

Exclusion Criteria:

* The Poor Health
* Be allergic to anesthetics

Primary outcomes:
measure: location of YAP in tissues
timeFrame: through study completion, an average of 2 years
measure: protein expression level of YAP in tissues
timeFrame: through study completion, an average of 2 years
Secondary outcomes:
measure: expression level of Th1 cytokines in the tissues
timeFrame: through study completion, an average of 2 years
measure: expression level of Th17 cytokines in the tissues
timeFrame: through study completion, an average of 2 years
measure: activation of hippo-YAP in NSECs on protein aspect
timeFrame: through study completion, an average of 2 years
measure: activation of hippo-YAP in NSECs
timeFrame: through study completion, an average of 2 years
measure: activation of hippo-YAP in NSECs on gene aspect
timeFrame: through study completion, an average of 2 years
Other outcomes:
Locations:
city: Guangzhou
country: China
facility: ThirdSunYatSen
state: Guangdong
Study officials:
References: