Clinical trial NCT03842371: Metabolism of Branched-chain Amino Acids in Monocyte/Macrophage During Sepsis
Official title: Metabolism of Branched-chain Amino Acids in Monocyte and Macrophage During Sepsis
Study type: OBSERVATIONAL
Overall status: UNKNOWN
Conditions: Sepsis Syndrome
Lead sponsor: West China Hospital
Sponsor class: OTHER
First posted: 2019-02-15
Enrollment: 90
Sex: ALL
Minimum age (years): 18
Maximum age (years): 70
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: yes
Observational model: CASE_CONTROL
Time perspective: PROSPECTIVE
Sampling method: PROBABILITY_SAMPLE
Study population: Patients who are diagnosed as sepsis, with or without type 2 diabetes.

Brief summary: The current project was designed to examine the metabolic level of branched-chain amino acids in plasm and monocyte/macrophage, and its role in immune dysfunction during sepsis.

Detailed description: In this study, the investigators will collect the peripheral blood of 1,3,7,14 day after diagnose of sepsis. Then, the concentration of branched-chain amino acids(BCAAs) in monocytes and plasma, and the expression of BCAAs metabolic enzymes in monocytes will be determined. Additionally, the inflammatory cytokines, e.g., Interleukin-1β (IL-1β), Tumor necrosis factor-α (TNF-α), Interleukin-10 (IL-10）, the chemokines macrophage inflammatory proteins 1α and 1β (MIP-1α and 1β) in the peripheral blood and the phagocytic function of monocytes will also be detected. The current project was designed to examine the metabolic level of branched-chain amino acids in plasm and monocyte/macrophage, and its role in immune dysfunction during sepsis.

Eligibility criteria: Inclusion Criteria:

* patients diagnosed with sepsis, with or without type 2 diabetes
* healthy volunteers

Exclusion Criteria:

* patients with immunodeficient diseases
* patients who accept glucocorticoid or immunosuppressant
* patients with an age younger than 18 years
* patients who cannot provide the informed consent

Primary outcomes:
measure: Intracellular levels of branched-chain amino acids in monocytes
timeFrame: up to 14 day after diagnose of sepsis
Secondary outcomes:
measure: Plasma levels of branched-chain amino acids
timeFrame: 1,3,5,7,10,14 day after diagnose of sepsis
measure: The expression of branched-chain amino acid aminotransferase in monocytes
timeFrame: 1,3,5,7,10,14 day after diagnose of sepsis
measure: The expression of branched-chain α-ketoacid dehydrogenase in monocytes
timeFrame: 1,3,5,7,10,14 day after diagnose of sepsis
measure: The expression of PP2Cm in monocytes
timeFrame: 1,3,5,7,10,14 day after diagnose of sepsis
measure: The plasma level of IL-1β
timeFrame: 1,3,5,7,10,14 day after diagnose of sepsis.
measure: The plasma level of TNF-α
timeFrame: 1,3,5,7,10,14 day after diagnose of sepsis.
measure: The plasma level of IL-10
timeFrame: 1,3,5,7,10,14 day after diagnose of sepsis.
measure: The plasma level of chemokine MIP-1α.
timeFrame: 1,3,5,7,10,14 day after diagnose of sepsis.
measure: The plasma level of chemokine MIP-1β.
timeFrame: 1,3,5,7,10,14 day after diagnose of sepsis.
measure: The phagocytic index of monocytes.
timeFrame: 1,3,5,7,10,14 day after diagnose of sepsis.
Other outcomes:
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