Clinical trial NCT05692752: Expression of microRNA-133a and microRNA-208b in Acute Myocardial Infarction
Official title: Expression of microRNA-133a and microRNA-208b in Acute Myocardial Infarction Egyptian Patients
Study type: OBSERVATIONAL
Overall status: COMPLETED
Conditions: Myocardial Infarction, Acute
Lead sponsor: Islamia University of Bahawalpur
Sponsor class: OTHER
Collaborators: name: German University in Cairo
First posted: 2023-01-20
Enrollment: 10
Sex: ALL
Minimum age (years): 35
Maximum age (years): 65
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: yes
Observational model: CASE_ONLY
Time perspective: PROSPECTIVE
Sampling method: NON_PROBABILITY_SAMPLE
Study population: Patient registering at the Coronary Care Unit at Ain-shams University hospital German University in Cairo, New Cairo City,.Egypt

Brief summary: Cardiac-enriched micro-RNAs (miRNAs), micro RNA 208b and 133a(MiR-208b, MiR-133a)) corresponds to the health and disorders of the cardiovascular system. An intron of the cardiac myosin heavy chain gene MYH7 encodes miR-208b. It is found on chromosome 14 in humans. Identify new diagnostic biomarkers based on miRNAs, researchers examine the expression of miR-133a and 208b at various time points (04 hours, 08 hours, 12 hours, 24 hours, 48 hours) following the development of the infarct and compared it to the traditional myocardial infarction biomarkers cardiac troponine (cTnl) and Creatine kinase-MB (CK-MB).

Detailed description: The 98% of human genes are non-coding RNA, which is further divided into long non-coding RNA (lncRNA, \> 200 bp) and short non-coding RNA (200 bp, including microRNA (miRNA) and circular RNA). More than 2000 human miRNAs have been discovered, and it seems that they regulate around 60% of human genes.Through a number of pathways, miRNAs control the development, re-modelling, and regeneration of the heart as well as endothelial function, vasculo-genesis, and neoangiogenesis. 30% of the genes in the human genome are regulated by about 1000 miRNAs. Endogenous miRNAs are 19-22 nucleotide single-stranded RNA molecules that frequently target the 3' untranslated regions (3'-UTR) of mRNAs to regulate gene expression at the post-transcriptional level in physiological processes, disease development, and conditions like cancer, autoimmune and inflammatory diseases, neuro-degenerative diseases, and cardiovascular diseases. Cardiac-enriched miRNAs, MiR-208 in health and disorders of the cardiovascular system are investigated.

Identify new diagnostic biomarkers based on miRNAs, researchers examine the expression of miR-133a and 208b at various time points (04 hours, 08 hours, 12 hours, 24 hours, 48 hours) following the development of the infarct and compared it to the traditional myocardial infarction biomarkers cTnl and CK-MB.

Eligibility criteria: Inclusion Criteria:

1. Patients diagnosed with ST-Elevated Myocardial Infarction will be identified at the Coronary Care Unit at Ain-shams University hospital
2. Acute episode of Myocardial Infarction
3. With No history of previous Myocardial Infarction Treatment

Exclusion Criteria:

1. Patients not diagnosed with ST-Elevated Myocardial Infarction and healthy at the Coronary Care Unit at Ain-shams 2. University hospital
2. Previously Known patients of Myocardial infacrtion
3. Previous medication

Primary outcomes:
measure: Expression of miR-133a
timeFrame: 04 hours from the onset of the infarct
measure: Expression of miR-133a
timeFrame: 08 hours from the onset of the infarct
measure: Expression of miR-133a
timeFrame: 12 hours from the onset of the infarct
measure: Expression of miR-133a
timeFrame: 24 hours from the onset of the infarct
measure: Expression of miR-133a
timeFrame: 48 hours from the onset of the infarct
measure: Expression of miR-208b
timeFrame: 04 hours from the onset of the infarct
measure: Expression of miR-208b
timeFrame: 08 hours from the onset of the infarct
measure: Expression of miR-208b
timeFrame: 12 hours from the onset of the infarct
measure: Expression of miR-208b
timeFrame: 24 hours from the onset of the infarct
measure: Expression of miR-208b
timeFrame: 48 hours from the onset of the infarct
Secondary outcomes:
Other outcomes:
Locations:
city: Cairo
country: Egypt
facility: Biochemistry Department, Faculty of Pharmacy and Biotechnology, German University in Cairo, Main Entrance Al Tagamoa Al Khames
Study officials:
affiliation: Biochemistry Department German University in Cairo, 11835, Cairo, Egypt
name: Sally I Hassanien, Ph.D
role: PRINCIPAL_INVESTIGATOR
affiliation: University College of Conventional Medicine, The Islamia University of Bahawalpur, Punjab, Pakistan
name: Hafiz A Sattar, M.Phil
role: PRINCIPAL_INVESTIGATOR
References: