Clinical trial NCT02700698: Mitochondrial Function in Circulating Cells and Muscle Tissue
Official title: Mitochondrial Function in Circulating Cells and Muscle Tissue
Study type: OBSERVATIONAL
Overall status: COMPLETED
Conditions: Healthy
Obesity
Insulin Sensitivity
Women
Keywords: Mitochondria
women
muscle
PBMC
Lead sponsor: University of Arkansas
Sponsor class: OTHER
Collaborators: name: Arkansas Children's Hospital Research Institute
First posted: 2016-03-07
Enrollment: 35
Sex: FEMALE
Minimum age (years): 25
Maximum age (years): 35
Standard ages: ADULT
Accepts healthy volunteers: yes
Observational model: COHORT
Time perspective: CROSS_SECTIONAL
Sampling method: PROBABILITY_SAMPLE
Study population: Twenty (20) healthy lean and 20 insulin resistant and obese women, age 25-35 years.

Brief summary: The main goal of this project is to determine whether mitochondrial function in circulating cells is related to that measured in the muscle fibers of the same subjects.

Detailed description: Data from human and animal models strongly suggests that reduced tissue mitochondrial function can be an early marker for detection of dysfunctional metabolism. It is unclear if tissue mitochondrial dysfunction can be detected using less invasive methodology. In this project, the investigators plan to study mitochondrial bioenergetics in circulating cells and paired muscle biopsies in two metabolic disparate groups of women; healthy lean vs. overweight/obese with/without insulin resistant women of similar age. Women of similar age that are either healthy lean or overweight/obese with/without insulin resistance will be recruited to participate in one study that includes a blood draw and a muscle biopsy. Mitochondrial function in circulating blood cells and muscle tissue will be determined. The association between circulating blood cells and muscle tissue mitochondria respiration will be determined for the two groups combined, and differences between groups will also be investigated.

Eligibility criteria: Inclusion Criteria:

* Women
* 25-35 years of age
* Either healthy lean (BMI\<25) or insulin resistant and obese (BMI \>30)

Exclusion Criteria:

* Medication that can affect study outcomes, as determined by the principal investigator, including anti-diabetic medication
* Chronic diseases, acute inflammation, chronic renal failure (glomerular filtration rate \<45), or heart problems

Primary outcomes:
measure: Mitochondrial Function in Circulating Cells and Muscle Tissue in Young Women
timeFrame: During the visit, approximately 0.5 days
Secondary outcomes:
Other outcomes:
Locations:
city: Little Rock
country: United States
facility: Arkansas Children's Nutrition Center
state: Arkansas
city: Little Rock
country: United States
facility: UAMS
state: Arkansas
Study officials:
affiliation: Arkansas Children's Nutrition Center
name: Elisabet Borsheim, PhD
role: PRINCIPAL_INVESTIGATOR
References:
citation: Rose S, Carvalho E, Diaz EC, Cotter M, Bennuri SC, Azhar G, Frye RE, Adams SH, Borsheim E. A comparative study of mitochondrial respiration in circulating blood cells and skeletal muscle fibers in women. Am J Physiol Endocrinol Metab. 2019 Sep 1;317(3):E503-E512. doi: 10.1152/ajpendo.00084.2019. Epub 2019 Jun 18.