Clinical trial NCT04902833: Acquired Pyruvate Kinase Deficiency In Clonal Myeloid Neoplasms
Official title: Characterization Of Acquired Pyruvate Kinase Deficiency In Clonal Myeloid Neoplasms
Study type: OBSERVATIONAL
Overall status: RECRUITING
Conditions: Pyruvate Kinase Deficiency
Pyruvate Kinase Deficiency Anemia
Hereditary Hemolytic Anemia
Myelodysplastic Syndromes
Myelodysplastic/Myeloproliferative Neoplasm
Clonal Myeloid Neoplasm
Myeloproliferative Neoplasm
Acute Myeloid Leukemia
Clonal Cytopenia of Undetermined Significance
Other Clonal Myeloid Neoplasm
Unexplained Coombs-negative Non-immune Hemolytic Anemia
Keywords: Pyruvate Kinase Deficiency
Pyruvate Kinase Deficiency Anemia
Hereditary Hemolytic Anemia
Myelodysplastic Syndromes
Myelodysplastic/Myeloproliferative Neoplasm
Clonal myeloid neoplasm
Myeloproliferative Neoplasm
Acute Myeloid Leukemia
Clonal Cytopenia of Undetermined Significance
Other clonal myeloid neoplasm
Unexplained Coombs-negative non-immune hemolytic anemia
Lead sponsor: Massachusetts General Hospital
Sponsor class: OTHER
Collaborators: name: Agios Pharmaceuticals, Inc.
First posted: 2021-05-26
Enrollment: 100
Sex: ALL
Minimum age (years): 18
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: no
Observational model: COHORT
Time perspective: PROSPECTIVE
Sampling method: PROBABILITY_SAMPLE
Study population: Cohort 1 will recruit approximately 50 anemic (Hgb \<11.0 g/dL) MDS patients without overt clinical evidence of hemolysis. Cohort 2 will recruit approximately 50 patients with clonal myeloid disorders of any type with evidence of non-immune, otherwise unexplained hemolytic anemia.

Brief summary: This cross-sectional prevalence assessment study involves a single blood draw in specific patient populations to assess for enzymatic and genomic evidence for acquired pyruvate kinase deficiency.

Detailed description: This cross-sectional prevalence assessment study involves a single blood draw in specific patient populations to assess for enzymatic and genomic evidence for acquired pyruvate kinase deficiency.

* Red cell pyruvate kinase enzyme activity and next-generation sequencing (NGS) hereditary hemolytic anemia panels will be performed on samples from all recruited participants.
* The study will recruit patients to two separate cohorts.

  * Cohort 1 will recruit approximately 75 anemic (Hgb \<11.0 g/dL) MDS participants without overt clinical evidence of hemolysis.
  * Cohort 2 will recruit approximately 25 participants with clonal myeloid disorders of any type with evidence of non-immune, otherwise unexplained hemolytic anemia
* Participation in the study involves a single blood draw. Basic information about the participant's blood disorder will also be collected.

It is expected that about 100 people will take part in this research study

Eligibility criteria: Inclusion Criteria:

* Cohort 1

  * Capable and willing to provide informed consent for participation in the study.
  * Diagnosis of clonal cytopenia of undetermined significance (CCUS), myelodysplastic syndrome (MDS) or myelodysplastic/myeloproliferative neoplasm (MDS/MPN syndrome) according to 2016 World Health Organization (WHO) classification system.
  * Anemia secondary to underlying clonal cytopenia of undetermined significance (CCUS), MDS or MDS/MPN syndrome, defined as a hemoglobin \<11.0 g/dL measured within 30 days of study enrollment. Anemia should not be related to nutritional deficiency (such as iron, cobalamin, folate, or copper deficiencies), peripheral immune or non-immune hemolysis, or renal disease, in the opinion of the investigator.
  * Age \>18 years.
* Cohort 2

  * Capable and willing to provide informed consent for participation in the study.
  * Diagnosis of a clonal myeloid neoplasm, such as MDS, MDS/MPN syndrome, myeloproliferative neoplasm (MPN), acute myeloid leukemia (AML), clonal cytopenia of undetermined significance (CCUS), or other clonal myeloid neoplasm according to 2016 World Health Organization (WHO) classification system.
  * A diagnosis of an otherwise unexplained Coombs-negative non-immune hemolytic anemia, according to the clinical judgement of the investigator. Some form of objective laboratory evidence must be present, including one or more of the following: negative direct antiglobulin (Coombs) test, reduced haptoglobin, elevated indirect bilirubin, elevated lactate dehydrogenase, elevated aspartate aminotransferase, or compatible findings on peripheral blood film. Results of all of these tests are not required to satisfy this criterion.
  * Age \>18 years.

Exclusion Criteria:

* Cohort 1

  * Receipt of red cell transfusion within 60 days of study enrollment.
  * Have a known untreated nutritional anemia or acquired disorder resulting in hemolysis, such as paroxysmal nocturnal hemoglobinuria (PNH). A known hereditary anemia (such as thalassemia trait) is not exclusionary if the patient's baseline hemoglobin has worsened significantly (in the opinion of the investigator) after development and diagnosis of MDS.
* Cohort 2

  * Have a known hereditary anemic disorder, such as thalassemia, sickle cell disease, or hereditary enzyme deficiency, with the exception of hereditary X-linked glucose-6-phosphate dehydrogenase deficiency known not to cause chronic baseline hemolysis. Testing for these diagnoses is not required unless deemed clinically necessary.
  * Have a known untreated nutritional anemia or acquired disorder resulting in hemolysis, such as paroxysmal nocturnal hemoglobinuria (PNH).

Primary outcomes:
measure: Overall prevalence of possible or likely acquired pyruvate kinase deficiency
timeFrame: Day 1
Secondary outcomes:
measure: Overall prevalence of definite acquired pyruvate kinase deficiency
timeFrame: Day 1
measure: Red cell pyruvate kinase enzyme activity
timeFrame: 60 days
measure: Red cell pyruvate kinase
timeFrame: 60 Day
measure: Somatic mutations in PKLR (and other genes associated with acquired PKD) detected in the hematopoietic clone
timeFrame: Day 1
measure: Somatic mutations in other genes associated with hemolytic anemia detected in the hematopoietic clone
timeFrame: Day 1
measure: Characterization of pyruvate kinase-related red cell metabolites (ATP, 2,3-DPG) and pyruvate kinase-R protein in patients with clonal myeloid disorders
timeFrame: Day 1
measure: Impact of pyruvate kinase activators on PK activity in vitro
timeFrame: Day 1
Other outcomes:
measure: Characterization of other possible factors involved in acquired PKD, including acquired epigenetic or gene expression factors
timeFrame: Day 1
Locations:
city: Boston
country: United States
facility: Massachusetts General Hospital
state: Massachusetts
Study officials:
affiliation: Massachusetts General Hospital
name: Hanny Al-Samkari, MD
role: PRINCIPAL_INVESTIGATOR
References: