Clinical trial NCT06561971: Effect of Different Centrifuge Values on Saliva High Sensitive C-Reactive Protein (Hs-CRP) Levels in Individuals With Different Smoking Amounts
Official title: Evaluating the Effect of Smoking Amount on Determining Salivary High Sensitive C-Reactive Protein(Hs-CRP) Values by Centrifuging the Saliva of the Same Individuals at Different Rpm and Time.
Study type: INTERVENTIONAL
Overall status: RECRUITING
Phase: NA
Conditions: Smoking
Lead sponsor: Izmir Katip Celebi University
Sponsor class: OTHER
First posted: 2024-08-20
Enrollment: 90
Sex: ALL
Minimum age (years): 18
Maximum age (years): 30
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: NON_RANDOMIZED
Intervention model: PARALLEL
Intervention model description: According to full-mouth clinical periodontal measurements including probing depth (PD), clinical attachment level (CAL),bleeding on probing (BOP), gingival index (GI) and plaque index (PI) samples of saliva were obtained from 90 systemically healthy non-smoker individuals with heavy smokers (H, n=30), light smokers(L, n=30) and non-smokers (N, n=30).
Primary purpose: DIAGNOSTIC

Brief summary: The purpose of this study; In periodontally healthy individuals, high sensitive c-reactive protein (hs-CRP) levels of non-smokers, smokers of less than 10 cigarettes per day and smokers of more than 10 cigarettes per day were measured at 15 rpm-10 min, 10 centrifuge values, which are frequently used in saliva studies. To determine the levels in saliva samples as a result of rpm-10 min and 10 rpm 6 min centrifugations and to determine the values at which a cytokine of this molecular weight should be centrifuged and to examine the possible correlation between these values and the clinical parameters of the amount of smoking. Materials and Methods: saliva samples were collected from 90 systemically healthy individuals who were non-smokers (N, n=30), light smokers \<10 cigarettes per day (L, n=30) and heavy smokers \>10 cigarettes per day (H, n=20). samples were taken. Full-mouth clinical periodontal measurements, including probing depth (PD), clinical attachment level (CAL), bleeding on probing (BOP), gingival index (GI), and plaque index (PI), were also recorded. Enzyme-linked immunosorbent assay (ELISA) was used to determine hs-CRP levels in biological samples.


Eligibility criteria: Inclusion Criteria:

* Systemically healthy
* Periodontally clinical healthy
* At least 20 permanent teeth in the mouth
* No medication for continuous use
* Those who have not used antibiotics, anti-inflammatory and systemic corticosteroid drugs in the last 6 months.
* Not in pregnancy or lactation period.
* Not received periodontal treatment in the last 6 months

  * For the non-smoker group; person who does not smoke tobacco
  * For the light-smoker group; person who smokes less than 10 cigarettes a day
  * For heavy-smoker group; person who smokes more than 10 cigarettes a day

Exclusion Criteria:

* Any oral or systemic disease
* Regularly using a systemic medication
* During pregnancy or lactation
* Received periodontal treatment within the last 6 months.
* Those who received antibiotic, anti-inflammatory or systemic corticosteroid medication in the last 6 months
* Individual with periodontal disease

Primary outcomes:
measure: The total amount of hs-CRP in saliva
timeFrame: 24 hours after taking the clinical measurements at the first visit
Secondary outcomes:
measure: Determining the ideal centrifugal value range of hs-CRP saliva level
timeFrame: Within 1 week after receiving the biochemical analysis results
Other outcomes:
Locations:
city: Izmir
country: Turkey
facility: Izmir Katip Çelebi University Department of Periodontology
state: Çiğli
Study officials:
References: