Clinical trial NCT05932771: Resistance Exercise and Hydrolyzed Collagen Supplementation
Official title: The Dose-response of Vitamin C-enriched Collagen on Markers of Collagen Synthesis in Healthy Young and Older Men and Women Following Resistance Exercise
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Diet, Food, and Nutrition
Keywords: Hydolyzed collagen
Resistance exercise
Collagen synthesis
Proline
Glycine
Lead sponsor: Liverpool John Moores University
Sponsor class: OTHER
Collaborators: name: University of East Anglia
First posted: 2023-07-06
Enrollment: 21
Sex: ALL
Minimum age (years): 18
Maximum age (years): 65
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: CROSSOVER
Intervention model description: A double-blind, randomised cross-over design
Primary purpose: BASIC_SCIENCE

Brief summary: This study aimed to investigate the effect of different doses of hydrolyzed collagen (HC) with resistance exercise (RE) on whole body collagen synthesis in healthy young and older males and females.

Detailed description: This study aimed to investigate the effect of different doses of hydrolyzed collagen (HC) with resistance exercise (RE) on whole body collagen synthesis in healthy young and older males and females.

Healthy young and older males and females ingested 0 grams, 15 grams or 30 grams HC with 50 milligrams vitamin C 1h prior to performing four sets' barbell back-squat RE at 10-repetition maximum load, after which they rested for six hours. Blood samples were collected throughout each trial to analyse procollagen type Ⅰ N-terminal propeptide (PⅠNP, a biomarker of collagen synthesis) and β-isomerized C-terminal telopeptide of type I collagen (β-CTX, a biomarker of collagen breakdown) concentration, and the concentration of 18 amino acids that constitute collagen.

This is the first study to investigate the combined effect of different doses HC with high-intensity RE on whole body collagen synthesis in healthy young and older men and women. If 30 grams HC intake with RE does augment collagen synthesis more than RE alone, this suggests that long-term HC intake with chronic RE would be beneficial for tendon health, because collagen is the most abundant protein in tendon.

Eligibility criteria: Inclusion Criteria:

* At least 12 months experienced in resistance exercise (regular back squat experience)
* Free from musculoskeletal injury
* Nullipara (a woman who has never given birth) (only for young female participatns)

Exclusion Criteria:

* Having a history of patellar tendon pathology
* Vegan
* Consumed nutritional supplements or medication purported to have beneficial effects on muscle-tendon properties (e.g. antioxidants, protein, etc.)
* Had a sustained a lower limb injury in the previous six months
* Smoker/vaper
* \<18 or \>40 years old (only for young participants)
* \<40 ro \>65 years old (only for older participants)
* Having an irregular menstrual cycle (only for young female participants)

Primary outcomes:
measure: Change in a marker of collagen synthesis
timeFrame: at rest immediately prior to HC ingestion, 0.5-hour post RE, 1-hour post RE, 2-hour post RE, 4-hour post RE and 6-hour post RE
measure: Change in a marker of collagen breakdown
timeFrame: at rest immediately prior to HC ingestion and 6-hour post RE
measure: Changes in amino acids concentrations in blood
timeFrame: at rest immediately prior to HC ingestion, 0.5-hour post HC ingestion, 1-hour post HC ingestion, 0.5-hour post RE, 1-hour post RE, 2-hour post RE, 4-hour post RE and 6-hour post RE
Secondary outcomes:
measure: Estrogen concentrations in women in each trial
timeFrame: at rest immediately prior to HC ingestion
Other outcomes:
Locations:
city: Liverpool
country: United Kingdom
facility: Dr Rob Erskine
Study officials:
affiliation: Liverpool John Moores University
name: Robert M. Erskine, PhD
role: STUDY_DIRECTOR
affiliation: Liverpool John Moores University
name: Joonsung Lee, MSc
role: PRINCIPAL_INVESTIGATOR
affiliation: Liverpool John Moores University
name: Christopher Nulty
role: PRINCIPAL_INVESTIGATOR
References: