Clinical trial NCT06043089: Impact of Beetroot Supplementation in Athletes
Official title: Impact of Beetroot Supplementation on Sports Performance, Prooxidant-antioxidant Balance and Sleep in Athletes
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Healthy
Lead sponsor: University School of Physical Education, Krakow, Poland
Sponsor class: OTHER
Collaborators: name: University of Bergen
First posted: 2023-09-21
Enrollment: 20
Sex: MALE
Minimum age (years): 14
Maximum age (years): 23
Standard ages: CHILD
ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: CROSSOVER
Primary purpose: TREATMENT

Brief summary: Several previous studies observed a positive effect of beetroot supplementation on sports performance in athletes, mainly in endurance performance. However, less attention has been paid to its effects on short-duration performance, especially in youth athletes. Moreover, only a few previous investigations performed biochemical analyses, including pro-oxidant balance assessments. It is especially important because chronic nitrate intake might directly cause the production of reactive nitrogen species and reactive oxygen species in other subcellular compartments, leading to more oxidative stress. Additionally, no previous study verified the impact of beetroot intake on sleep.


Eligibility criteria: Inclusion Criteria:

* male
* good general health assessed by physician
* highly trained
* written consent to participate

Exclusion Criteria:

* medication or dietary supplements used within the previous month, which could potentially impact the study outcomes
* history of sleep nor neurological disorders
* chronic use (6 months) of any antibacterial mouthwash products.

Primary outcomes:
measure: Performance test
timeFrame: 7 days after treatment
measure: Changes in nitrates
timeFrame: 1) baseline - 2) 7th day of treatment - before the supplementation, 3) 7th day of treatment - before the performance test, 4) 7th day of treatment - immediately after the performance test
measure: Changes in nitrites
timeFrame: 1) baseline - 2) 7th day of treatment - before the supplementation, 3) 7th day of treatment - before the performance test, 4) 7th day of treatment - immediately after the performance test
measure: Changes in prooxidant status
timeFrame: 1) baseline - 2) 7th day of treatment - before the supplementation, 3) 7th day of treatment - before the performance test, 4) 7th day of treatment - immediately after the performance test
measure: Changes in antioxidant status
timeFrame: 1) baseline - 2) 7th day of treatment - before the supplementation, 3) 7th day of treatment - before the performance test, 4) 7th day of treatment - immediately after the performance test
measure: Changes in oxidative stress index
timeFrame: 1) baseline - 2) 7th day of treatment - before the supplementation, 3) 7th day of treatment - before the performance test, 4) 7th day of treatment - immediately after the performance test
measure: Changes in nitrooxidative stress markers
timeFrame: 1) baseline - 2) 7th day of treatment - before the supplementation, 3) 7th day of treatment - before the performance test, 4) 7th day of treatment - immediately after the performance test
measure: Changes in protein oxidation
timeFrame: 1) baseline - 2) 7th day of treatment - before the supplementation, 3) 7th day of treatment - before the performance test, 4) 7th day of treatment - immediately after the performance test
measure: Changes in nucleic acid oxidation
timeFrame: 1) baseline - 2) 7th day of treatment - before the supplementation, 3) 7th day of treatment - before the performance test, 4) 7th day of treatment - immediately after the performance test
measure: Changes in lipids oxidation
timeFrame: 1) baseline - 2) 7th day of treatment - before the supplementation, 3) 7th day of treatment - before the performance test, 4) 7th day of treatment - immediately after the performance test
measure: Changes in exercise-induced metabolic acidosis
timeFrame: 1) Before the performance test 2) 3 minutes after the performance test 3) 10 minutes after the performance test 4) 20 minutes after the performance test
measure: Objective Sleep - Time in bed
timeFrame: nights 1 - 7 during treatment
measure: Objective Sleep - Sleep-onset latency
timeFrame: nights 1 - 7 during treatment
measure: Objective Sleep - Total sleep time
timeFrame: nights 1 - 7 during treatment
measure: Objective Sleep - Light sleep
timeFrame: nights 1 - 7 during treatment
measure: Objective Sleep - Deep sleep
timeFrame: nights 1 - 7 during treatment
measure: Objective Sleep - REM sleep
timeFrame: nights 1 - 7 during treatment
measure: Objective Sleep - Sleep efficiency
timeFrame: nights 1 - 7 during treatment
Secondary outcomes:
measure: Countermovement jump height
timeFrame: 7 days after treatment
measure: Ball speed velocity
timeFrame: 7 days after treatment
measure: Sprint test (0-20m)
timeFrame: 7 days after treatment
measure: Agility T-test
timeFrame: 7 days after treatment
measure: Maximal isometric handgrip strength
timeFrame: 7 days after treatment
measure: Flanker task
timeFrame: 7 days after treatment
measure: Changes in muscle damage markers
timeFrame: 1) baseline - 2) 7th day of treatment - before the supplementation, 3) 7th day of treatment - before the performance test, 4) 7th day of treatment - immediately after the performance test
measure: Changes in muscle enzymes activity
timeFrame: 1) baseline - 2) 7th day of treatment - before the supplementation, 3) 7th day of treatment - before the performance test, 4) 7th day of treatment - immediately after the performance test
measure: Changes in inflammation markers
timeFrame: 1) baseline - 2) 7th day of treatment - before the supplementation, 3) 7th day of treatment - before the performance test, 4) 7th day of treatment - immediately after the performance test
measure: Changes in blood count
timeFrame: 1) baseline - 2) 7th day of treatment - before the supplementation, 3) 7th day of treatment - before the performance test, 4) 7th day of treatment - immediately after the performance test
measure: Changes in anabolic hormones
timeFrame: 1) baseline - 2) 7th day of treatment - before the supplementation, 3) 7th day of treatment - before the performance test, 4) 7th day of treatment - immediately after the performance test
measure: Changes in catabolic hormones
timeFrame: 1) baseline - 2) 7th day of treatment - before the supplementation, 3) 7th day of treatment - before the performance test, 4) 7th day of treatment - immediately after the performance test
measure: Changes in kidney function markers
timeFrame: 1) baseline - 2) 7th day of treatment - before the supplementation, 3) 7th day of treatment - before the performance test, 4) 7th day of treatment - immediately after the performance test
measure: Changes in the purine metabolism marker
timeFrame: 1) baseline - 2) 7th day of treatment - before the supplementation, 3) 7th day of treatment - before the performance test, 4) 7th day of treatment - immediately after the performance test
measure: Changes in hemoglobin concentration
timeFrame: 1) Before the performance test 2) 3 minutes after the performance test 3) 10 minutes after the performance test 4) 20 minutes after the performance test
measure: Changes in hematocrit
timeFrame: 1) Before the performance test 2) 3 minutes after the performance test 3) 10 minutes after the performance test 4) 20 minutes after the performance test
measure: Changes in plasma volume
timeFrame: 1) Before the performance test 2) 3 minutes after the performance test 3) 10 minutes after the performance test 4) 20 minutes after the performance test
Other outcomes:
Locations:
city: Kraków
country: Poland
facility: University School of Physical Education in Cracow
Study officials:
affiliation: University of Physcial Education in Cracow
name: Aleksandra Filip-Stachnik, Phd
role: PRINCIPAL_INVESTIGATOR
References: