Clinical trial NCT06096532: Adipose Tissue Blood Flow in Aging Humans
Official title: Adipose Tissue Blood Flow in Aging Humans
Study type: INTERVENTIONAL
Overall status: RECRUITING
Phase: NA
Conditions: Healthy
Aging
Lead sponsor: Mayo Clinic
Sponsor class: OTHER
Collaborators: name: National Center for Advancing Translational Sciences (NCATS)
First posted: 2023-10-23
Enrollment: 24
Sex: ALL
Minimum age (years): 18
Maximum age (years): 80
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: yes
Allocation: NON_RANDOMIZED
Intervention model: PARALLEL
Primary purpose: BASIC_SCIENCE

Brief summary: The goal of this basic science clinical trial is to understand whether adipose tissue blood flow changes between younger and older healthy adults.

The main question\[s\] it aims to answer are:

* Is basal and nutrient responsive adipose tissue blood flow (ATBF) different between younger and older adults
* What molecular and systemic signatures related to adipose tissue blood flow are altered between these two groups.

Participants will undergo measurements of adipose tissue blood flow using the xenon washout technique, undergo 2 abdominal subcutaneous adipose tissue biopsies, and drink a sugary drink.

Detailed description: On Visit 1, volunteers will provide written informed consent (and urine pregnancy test for women of childbearing age), complete a Dual x-ray absorptiometry scan, baseline labs (comprehensive metabolic panel) and a cardiopulmonary exercise test. Participants will arrive fasted at 08:00, and urine pregnancy test for women of childbearing age administered and an intravenous catheter will be placed. Following 30min of supine rest, baseline venous blood samples will be collected. ATBF will be assessed on participants' left abdomen first by measurement of blood flow using 133Xenon washout method. Immediately thereafter, an abdominal adipose tissue biopsy of the left side will be performed but ≥6 cm distant from location of 133Xenon injections. Subjects will them consume 75 gm glucose; because ATBF peaks 30-60 minutes following ingestion of glucose, ATBF measures will start 30 minutes after ingestion on the contralateral side followed immediately by plasma measures and another adipose tissue biopsy. During ATBF measurements, brachial blood pressure will be assessed in two-minute intervals.

Eligibility criteria: Inclusion criteria

* Within the ages of 18-35 years or 65-80 years at time of study visit
* BMI between 20-25.0 kg/m2.

Exclusion criteria:

* Presence of diagnosed cardiometabolic diseases (e.g., Type 2 diabetes, hypertension, Heart Failure)
* Taking prescription anticoagulants
* Taking prescriptions or supplements that effect adipose tissue metabolism (i.e., statins, Thiazolidinediones, niacin, atypical antipsychotics, or fish oil)
* Pregnant or nursing
* Inability to provide written informed consent
* Smokers

Primary outcomes:
measure: Adipose tissue blood flow
timeFrame: Baseline, pre-intervention fasting and immediately following 75 gm oral glucose load
Secondary outcomes:
measure: Genomic alterations
timeFrame: Baseline, pre-intervention fasting and immediately following 75 gm oral glucose load
Other outcomes:
Locations:
city: Rochester
country: United States
facility: Mayo Clinic Minnesota
state: Minnesota
Study officials:
affiliation: Mayo Clinic
name: Kelli Lytle, PhD
role: PRINCIPAL_INVESTIGATOR
References: