Clinical trial NCT02418728: The Role of Atrial Natriuretic Peptide in the Lipolytic Process: Effect of Obesity and Exercise
Official title: The Contribution of Atrial Natriuretic Peptide in Lipid Mobilization of Subjects With Obesity, Both During Rest and Exercise
Study type: OBSERVATIONAL
Overall status: COMPLETED
Conditions: Obesity
Keywords: obesity
adipose tissue lipolysis
lipolysis
microdialysis
atrial natriuretic peptide
Lead sponsor: Hasselt University
Sponsor class: OTHER
Collaborators: name: Jessa Hospital
name: Maastricht University
First posted: 2015-04-16
Enrollment: 30
Sex: MALE
Minimum age (years): 35
Maximum age (years): 60
Standard ages: ADULT
Accepts healthy volunteers: yes
Observational model: COHORT
Time perspective: RETROSPECTIVE
Sampling method: NON_PROBABILITY_SAMPLE
Study population: subjects with obesity

Brief summary: In the developing countries, obesity prevalence is on a dramatic rise. Obesity is related to co-morbidities and as a result, obesity significantly shortens life expectancy and lowers quality of life. To prevent this, participation in exercise or training programs is absolutely necessary, in order to generate adipose tissue mass loss. The amount of adipose tissue mass loss is, amongst others, dependent on lipolysis which is under endocrine regulation by, mainly, catecholamines, insulin and atrial natriuretic peptide. However, large variations in adipose tissue mass loss and gain are likely in obese subjects, possibly due to a decreased lipolytic effect of these hormones (as was shown for catecholamines in the subcutaneous adipose tissue of obese subjects). However, the relative contribution of atrial natriuretic peptide in the lipolytic process remains elusive, particularly in subjects with obesity, which show an increased plasma expression of atrial natriuretic peptide.

The aim of the present study is to observe the contribution of atrial natriuretic peptide in the subcutaneous adipose tissue of obese subjects. This will be tested by measurements of extracellular glycerol levels (by microdialysis) in the subcutaneous adipose tissue in situ at rest and during endurance exercise under local beta- and alpha-blockade.

Eventually, the knowledge gained from this research will contribute to the optimization of exercise programs for people with obesity.


Eligibility criteria: Inclusion Criteria:

* Obese group: BMI \> 30 kg/m², sedentary (no regular physical activity last 6 months), insulin sensitive or insulin resistant
* Lean controls: BMI \> 18.5 kg/m² and \< 25 kg/m², regular physical activity, insulin sensitive

Exclusion Criteria:

* Regular glucose lowering medication or beta blockade medication
* Presence of chronical diseases
* Orthopedic or neurological problems

Primary outcomes:
measure: Subcutaneous adipose tissue microdialysis
timeFrame: week 1
measure: Atrial natriuretic peptide (ANP) Response
timeFrame: week 1
Secondary outcomes:
measure: Central insulin sensitivity
timeFrame: screening
measure: Echocardiography
timeFrame: Day 1
measure: Maximal oxygen uptake (ml/O2/kg/min)
timeFrame: Day 1
measure: Anthropometry
timeFrame: screening
measure: Abdominal subcutaneous adipose tissue biopsy
timeFrame: week 1
Other outcomes:
Locations:
city: Diepenbeek
country: Belgium
facility: Hasselt University
Study officials:
affiliation: Maastricht University
name: Ellen Blaak, prof. dr.
role: PRINCIPAL_INVESTIGATOR
affiliation: Hasselt University
name: Kenneth Verboven, drs
role: STUDY_CHAIR
References: