Clinical trial NCT04157556: The Effect of Maximal Exercise on MMP9 and TIMP1 and The Role of MMP9 -1562 C/T and TIMP1 372 T/C Polymorphisms
Official title: The Effect of Maximal Exercise on Matrix Metalloproteinase Enzyme and Its Inhibitor (MMP9 and TIMP1) and The Role of MMP9 -1562 C/T and TIMP1 372 T/C Polymorphisms
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: MMP9
TIMP1
MMP9 -1562 C/T
TIMP1 372 T/C
Lead sponsor: Dokuz Eylul University
Sponsor class: OTHER
Collaborators: name: Ege University
First posted: 2019-11-08
Enrollment: 86
Sex: MALE
Minimum age (years): 18
Maximum age (years): 35
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: NON_RANDOMIZED
Intervention model: PARALLEL
Intervention model description: There are 2 groups in the study: Athletes and sedentary people.They will apply the same acute exercise protocol (The Yo-Yo Intermittent Recovery Test Level 1). A blood test will conduct before and after the acute exercise protocol.
Primary purpose: BASIC_SCIENCE

Brief summary: Matrix metalloproteinases (MMPs) play a critical role in the remodeling and regeneration of skeletal muscle. Studies which investigate exercise and effects of MMPs and tissue inhibitors of matrix metalloproteinases (TIMPs) are contradictory. Also the role of MMP9 -1562 C/T and TIMP1 372 T/C polymorphisms on exercise are unclear. In accordance with this conditions the hypothesis of the study are as follows:

1. Levels of basal serum MMP9 and TIMP1 are different between athletes (volleyball, basketball, handball) and sedentary people.
2. Levels of serum MMP9 and TIMP1 response to maximal exercise are different between athletes (volleyball, basketball, handball) and sedentary people .
3. Relationship between levels of serum MMP9 and TIMP1 response to maximal exercise are different between athletes (volleyball, basketball, handball) and sedentary people.
4. Levels of basal serum MMP9 and TIMP1 are related to MMP9 -1562 C/T and TIMP1 372 T/C polymorphisms.
5. Relationship between levels of serum MMP9 and TIMP1 response to maximal exercise and MMP9 -1562 C/T and TIMP1 372 T/C polymorphisms are different between athletes (volleyball, basketball, handball) and sedentary people.


Eligibility criteria: Inclusion Criteria:

Group of athletes:

* Male basketball players who have been training regularly for at least last 3 months.
* Male volleyball players who have been training regularly for at least last 3 months.
* Male handball players who have been training regularly for at least last 3 months.

Group of sedentary people:

* Male individuals with similar physical characteristics to the group of athletes
* Male individuals who have not exercise regularly for at least last 3 months.

Exclusion Criteria(For two groups):

* Smoking regularly
* Drinking alcohol regularly
* Using drug and antioxidant substance
* Being obese,
* Being sick or having disability

Primary outcomes:
measure: Level of MMP9 and TIMP1
timeFrame: Yo-Yo Intermittent Recovery Test level 1 (the test performed until the subject is exhausted, the test lasts 10-20 minutes according to endurance capacity of the participants)
measure: MMP9 -1562 C/T, TIMP1 372 T/C polymorphisms
timeFrame: Yo-Yo Intermittent Recovery Test level 1 (the test performed until the subject is exhausted, the test lasts 10-20 minutes according to endurance capacity of the participants)
Secondary outcomes:
measure: Level of indicators of muscle damage and inflammation and oxidative stress
timeFrame: Yo-Yo Intermittent Recovery Test level 1 (the test performed until the subject is exhausted, the test lasts 10-20 minutes according to endurance capacity of the participants)
measure: Level of blood lipids and lipoproteins, glucose, urea, creatinine, hemogram
timeFrame: Yo-Yo Intermittent Recovery Test level 1 (the test performed until the subject is exhausted, the test lasts 10-20 minutes according to endurance capacity of the participants)
Other outcomes:
Locations:
city: İzmir
country: Turkey
facility: Ege University Faculty of Sports Science
Study officials:
affiliation: Ege University, Faculty of Sports Science
name: Faruk Turgay, associate professor
role: STUDY_CHAIR
References: