Clinical trial NCT04806178: Immunological Response of Bladder Cancer Patients Under BCG
Official title: Systemic Immunological Response of Bladder Cancer Patients Under Bacillus Calmette Guérin Treatment
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: PHASE3
Conditions: Bladder Cancer
Bacillus Calmette-Guerin
Keywords: BCG
Bladder cancer
Immunotherapy
Lead sponsor: University of Campinas, Brazil
Sponsor class: OTHER
Collaborators: name: Coordination for the Improvement of Higher Education Personnel
name: Pontifical University Catholic of Campinas
First posted: 2021-03-19
Enrollment: 30
Sex: ALL
Minimum age (years): 18
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: no
Allocation: RANDOMIZED
Intervention model: PARALLEL
Primary purpose: TREATMENT

Brief summary: Bladder cancer (BC) is one of the most common cancers worldwide and the most successful example of vaccine in cancer treatment, representing an efficient model for studying the importance of systemic and local immune mechanisms. Despite being the standard of treatment for the last 40 years, the exact mode of action of immunotherapy with the bacillus Calmette-Guérin (BCG) is still poorly defined. In a mechanistic study, the investigators intend to prospectively investigate immunological signatures, including immune-checkpoints, pre and post-treatment in patients with BC, and correlate the cytokines of the immune by-product and BCG administration pathway to understand the independent contributions of BCG priming (prior exposure to BCG) and crosstalk immunotherapy between tumor profiles and immune response of the patient. The proposed research strategy is justified by the need to identify subsets of patients who better respond to an intervention, or to predict why new immunotherapies and drugs may be successful or failed in clinical trials.

Detailed description: Recognizing patient-to-patient variability, key data scarcity, and insight into traditional reductionist therapy, the BCG model offers exceptionally compelling opportunities to understand how immune system behavior in health and disease emerges from local, systemic, genetic, epigenetic, cellular, and environmental modulating factors.

The application seeks to change the current clinical practice and research paradigms, by using new theoretical concepts, challenging bladder cancer patients with a highly effective, safe, and affordable immunotherapy, the gold standard in the last 40 years of NMIBC, and in light of new concepts and methodologies brought by the paradigm of immune-checkpoint inhibitors that justified the Nobel Prize in Physiology or Medicine in 2018.

The current proposal has the potential to impact the prognosis and identification of those who are unlikely to respond to immune-checkpoint inhibitors, scenarios in which important unanswered questions remain, particularly as this class of agents advances along the spectrum of non-metastatic disease.

In a mechanistic approach, patients diagnosed with NMIBC and with the indication for intravesical BCG treatment will be randomized to placebo versus a priming intradermic BCG 14 days before the intravesical treatment and followed up to 180 days.

The investigators will define important clinical paradigms:

1. The role of the priming effect on the immune system and better understanding of BCG immunotherapy, with a clear potential for improvement of bladder cancer treatment in NMIBC and MIBC scenarios;
2. The potential of BCG, a widely used vaccine, to improve or impair the results of new immunotherapies, given its long-lasting effect;
3. Rational to develop future treatment associations of BCG and immune-checkpoints. \\

Under the new immunological concepts, a better understanding of tumor-associated immune responses in BC patients could provide more informed clinical decisions and treatment optimization.

Considering the growing need of assessing the value of treatment at the expense of cost, part of our proposal strategy is to limit financial toxicity as an important issue in cancer treatment and new immunotherapies.

Eligibility criteria: Inclusion Criteria:

* NMIBC with the indication for intravesical BCG treatment;

Exclusion Criteria:

* Previous BCG treatment;
* Muscle invasive tumor.

Primary outcomes:
measure: Flow Cytometry
timeFrame: Day 0
measure: Flow Cytometry
timeFrame: Day 14
measure: Flow Cytometry
timeFrame: Day 21
measure: Flow Cytometry
timeFrame: Day 35
measure: Flow Cytometry
timeFrame: Day 49
measure: Flow Cytometry
timeFrame: Day 180
Secondary outcomes:
measure: Adverse Effects and Change from Baseline Voiding Symptoms
timeFrame: Day 21
measure: Adverse Effects and Change from Baseline Voiding Symptoms
timeFrame: Day 35
measure: Adverse Effects and Change from Baseline Voiding Symptoms
timeFrame: Day 49
Other outcomes:
Locations:
city: Campinas
country: Brazil
facility: Pontifical Catholic University of Campinas Hospital
state: SP
city: Campinas
country: Brazil
facility: Hospital das Clínicas Unicamp
state: SP
Study officials:
affiliation: University of Campinas
name: Leonardo O Reis, MD, PhD
role: PRINCIPAL_INVESTIGATOR
References:
citation: Ji N, Mukherjee N, Morales EE, Tomasini ME, Hurez V, Curiel TJ, Abate G, Hoft DF, Zhao XR, Gelfond J, Maiti S, Cooper LJN, Svatek RS. Percutaneous BCG enhances innate effector antitumor cytotoxicity during treatment of bladder cancer: a translational clinical trial. Oncoimmunology. 2019 May 25;8(8):1614857. doi: 10.1080/2162402X.2019.1614857. eCollection 2019.
citation: van Puffelen JH, Keating ST, Oosterwijk E, van der Heijden AG, Netea MG, Joosten LAB, Vermeulen SH. Trained immunity as a molecular mechanism for BCG immunotherapy in bladder cancer. Nat Rev Urol. 2020 Sep;17(9):513-525. doi: 10.1038/s41585-020-0346-4. Epub 2020 Jul 16.