Clinical trial NCT04737785: Central Nervous System Disorders Following Hematopoietic Stem Cell Transplantation
Official title: Central Nervous System Disorders Following Hematopoietic Stem Cell Transplantation: a Prospective Observational Trial From the Infectious Diseases Working Party and the Transplant Complications Working Party of the European Society for Blood and Marrow Transplantation
Study type: OBSERVATIONAL
Overall status: COMPLETED
Conditions: Central Nervous System Infections
Central Nervous System Complication
Infectious Disease of Nervous System
Blood Disease
Blood Cancer
Infection
Lead sponsor: European Society for Blood and Marrow Transplantation
Sponsor class: NETWORK
First posted: 2021-02-04
Enrollment: 252
Sex: ALL
Standard ages: CHILD
ADULT
OLDER_ADULT
Accepts healthy volunteers: no
Observational model: CASE_CONTROL
Time perspective: PROSPECTIVE
Sampling method: NON_PROBABILITY_SAMPLE
Study population: HSCT patients of any age with any underlying disease for which the transplant is given.

Brief summary: All patients undergoing allogeneic or autologous HSCT at the participating centres will be observed. Once a diagnosis of CNS disorder is made, additional data will be reported for these patients.

We will identify clinical and diagnostic characteristics such as cerebrospinal fluid (CSF) and neuroimaging patterns, risk factors, response to treatment (including novel antifungal agents such as isavuconazole) and outcome. In addition, risk factors for CNS disorders after allogeneic and autologous HSCT will be analyzed using a prospectively assessed matched control group. In the future, this study might be the basis for an interventional trial (e.g. using a prophylactic approach).

Detailed description: For each case patient (i.e. HSCT recipient with a CNS disorder) two control patients (1:2 allocation) should be analysed prospectively with the aim to get more insights into risk factors for CNS disorders. Hereby, the two patients transplanted subsequently to the corresponding case patient should be included as controls if they survive and do not develop a CNS disorder until the inclusion time point. Inclusion time points of controls should be determined by using the same delay between transplant and the onset of the CNS disorder of the corresponding case patient. Controls should also be stratified by centre, HSCT type (i.e. allogeneic vs. autologous) and age (adults vs. paediatrics). As for cases with CNS disorders, controls should also be evaluated regularly until last follow-up. To avoid bias and to estimate the incidence of infectious vs. non-infectious CNS disorders in autologous and allogeneic HSCT recipients accurately centres should also report patients with CNS disorders not included in this study (e.g. due to lack of informed consent or essential Med-A data). Centres are additionally asked by the data management office in Leiden/Netherlands for these data on a regularly basis.

Primary objectives

* Clinical and diagnostic characteristics of infectious and non-infectious CNS disorders following allogeneic or autologous HSCT
* Outcome 30 days after CNS disorder onset (cured vs. improved vs. stabilized vs. worsened vs. died due to CNS disorder vs. died by other cause)

Secondary objectives

* Incidence, timing, and distribution of infectious and non-infectious CNS disorders after HSCT
* Impact of development of CNS disorders on overall survival
* Risk factors for CNS disorders after allogeneic and autologous HSCT using a prospectively assessed matched control group
* Efficacy of treatment for different types of CNS disorders

Data Collection \& Statistical Analysis Plan Data collection will be performed by the IDWP data office (Leiden/Netherlands). Hereby, patient and transplant data are reported directly from the centres to the data office according to EBMT guidelines.

Important basic data for analysis (collected from Med-A and Med-C for both case patients and controls):

* Recipient/donor sex
* Recipient/donor age (at HSCT)
* Primary diagnosis (leading to HSCT)
* Status of the primary disease at the time of HSCT - remission (partial or complete)/relapse/relapse including CNS involvement/progression, stable disease, unknown)
* Prior CNS radiotherapy
* Prior intrathecal (antineoplastic) treatment
* Prior (antineoplastic) treatment (especially 'novel drugs´)
* Pre-existing medical conditions, e.g. chronic kidney disease, liver disease
* Recipient/donor serostatus of CMV, EBV, HHV-6, HSV, VZV, Toxoplasma spp.
* Type of transplant (allogeneic vs. autologous)
* Type of donor (MRD vs. haploidentical donor vs. other donor type)
* Stem cell source (CB vs. BM vs. PB)
* Type of conditioning (e.g. MAC vs. RIC, fludarabine-containing vs. other, TBI incl. dose), including details on absolute dose and dose adjustments of fludarabine
* TCD (yes vs. no), ATG (yes vs. no), alemtuzumab (yes vs. no) in conditioning and at study inclusion (day 0)
* Acute and chronic GvHD (at day 0, including grade)
* ECOG performance status at different time points
* Concomitant infections: e.g. paranasal sinusitis, results of blood cultures performed during the episode of CNS disorder, galactomannan, beta-D-glucan (peripheral blood, BAL etc.)
* Selected peripheral blood parameters, e.g. white blood cell count, absolute neutrophil count, duration of neutropenia \<0.5x109/L, absolute lymphocyte count, platelet count, sodium, potassium, calcium, phosphate, creatinine, urea nitrogen, ammonia, bilirubin, IgG concentration, creatinine, GFR

Specific data to be collected for patients with CNS disorders (see also Med-C for cases and appendix), e.g.:

* Recipient´s age at onset of the CNS disorder (day 0)
* Date of symptom onset of the CNS disorder
* Type of symptoms (e.g. seizures, hemiplegia, paraplegia, paresis, psychosis, vomiting, confusion/altered consciousness, fever)
* Date of diagnosis of the CNS disorder (e.g. CSF analysis)
* Clinical diagnosis of CNS infection (e.g. encephalitis, meningitis, meningoencephalitis, myelitis, abscess, leukoencephalopathy)
* Clinical diagnosis of non-infectious CNS disorder (e.g. metabolic/drug-induced disorder, posterior reversible encephalopathy syndrome, bleeding, thrombosis, ischemic stroke, CNS relapse of a underlying malignancy)
* Time interval between HSCT and symptom onset
* Time interval between symptom onset and diagnosis of CNS disorder
* Antimicrobial prophylaxis prior to onset of a CNS disorder
* Level of likelihood of the type of CNS disorder - the centres report the diagnostic tests performed to make a diagnosis of CNS disorder including i.e.: magnetic resonance imaging (MRI), MRI spectrometry, positron emission tomography/computed tomography (PET/CT), CT, electroencephalography (EEG), CSF analyses, microbiological studies (e.g., cultures, PCR, galactomannan, Candida mannan, beta-D-glucan, antibodies in peripheral blood, CSF or other materials such as brain biopsy), autopsy results
* Type and efficacy of treatment for the CNS disorder 30 days after onset (cured vs. improved vs. stabilized vs. worsened vs. died due to CNS disorder vs. died by other cause)
* Outcome (alive or death, including date, cause of death, CNS disorder-related death vs. other death cause) at the different study points

Specific data to be collected for patients without CNS disorder (see also Med-C for controls), e.g.:

* Time interval between HSCT and day 0 (inclusion time point, same delay between transplant and CNS disorder onset of the corresponding case)
* Date of study inclusion (i.e. day 0)
* Recipient´s age at study inclusion
* Occurrence of a CNS disorder after study inclusion
* Antimicrobial prophylaxis prior to study inclusion
* Outcome (alive or death, including date, cause of death) at the different study points

Statistical analysis Statistical methods used to compare groups for primary and secondary outcomes Primary endpoints: descriptive statistics will be performed to report the main characteristics (and 30 day outcome) of patients with infectious and non-infectious CNS disorders: absolute and percentage frequencies will be reported for categorical variables; median, IQR and range will be used for continuous variables.

Secondary endpoints: the cumulative incidence of infectious and non-infectious CNS disorders after HSCT will be estimated by the cumulative incidence method: infectious and non-infectious CNS disorders will be considered as events, whilst non-CNS relapse, non-CNS secondary malignancy and non-CNS death will be considered as competing event. Overall survival will be assessed using Kaplan-Meier curves, and the impact of the development of CNS disorders on overall survival will be assessed using time-dependent Cox models. To compute these endpoints, the data of all patients after HSCT will be obtained from the EBMT patient databases (e.g. ProMISe).

The risk factor analysis will be performed by a conditional logistic regression model. P-values \<0.05 will be considered statistically significant. The analyses will be performed using the software SAS/R v 9.4 or higher (SAS Institute Inc., Cary, NC, USA).

Methods for additional analyses, such as subgroup analyses and adjusted analyses The cumulative incidence of infectious and non-infectious CNS disorders after HSCT will be computed and reported separately for different transplant types (autologous vs. allogeneic - matched related donor vs. haploidentical donor vs. other donor type), and stem cell source (cord blood vs. bone marrow vs. peripheral blood) of HSCT and for different age categories (adults vs. paediatrics). These comparisons will be presented in terms of crude cumulative incidence curves, and differences between groups will be assessed using Gray's test. Multivariable analyses will be performed using cause-specific hazard models. Cox models for overall survival will include CNS disorders as time-dependent covariates. Candidate variables for these analyses will be obtained from a list of predefined variables, including (but not limited to) type of transplant (allogeneic vs. autologous HSCT), age, stem cell source, donor relationship, and use of T cell depletion. Potential effects of centres will be examined using random effects.

Risk factors for CNS disorders after allogeneic and autologous HSCT will be assessed using multivariable conditional logistic regression. Candidate variables for this analysis will be obtained from a list of predefined variables, including the stratification variables.

Model building will proceed as follows: each variable will be preliminarily assessed using univariable analyses. Factors associated with the response and significant at the 20% level in univariable analyses will be considered for inclusion in a multivariable model. Factors will be retained if significant at the 5% level, and interaction terms included (after assessment of main effects) if significant at a 10% level (with possible corresponding subgroup analyses used to illustrate differential effects).

Eligibility criteria: Inclusion Criteria:

Case group:

* received allogeneic or autologous HSCT between January 1st, 2021 and December 31st, 2022
* develop an infectious (any CTCAE grade) or relevant (CTCAE \>1°) non-infectious CNS disorder after HSCT in this period.

Control group:

* received allogeneic or autologous HSCT between January 1st, 2021 and February 28th, 2023
* who survive and do not develop a CNS disorder until the inclusion time point (day 0, defined as the same delay between transplantation and CNS disorder onset of the corresponding case)

Exclusion Criteria:

* Patients with missing essential Med-A data
* Patients not giving informed consent to report data to EBMT prior to initiation of transplant procedures

Primary outcomes:
measure: Clinical characteristics of infectious and non-infectious CNS disorders following allogeneic or autologous HSCT
timeFrame: 32 months
measure: Diagnostic characteristics of infectious and non-infectious CNS disorders following allogeneic or autologous HSCT
timeFrame: 32 months
measure: Efficacy of CNS treatment for different types of CNS disorders
timeFrame: 30 days
measure: Survival
timeFrame: 32 months
Secondary outcomes:
measure: Incidence of infectious and non-infectious CNS disorders after HSCT
timeFrame: 32 months
measure: Timing of infectious and non-infectious CNS disorders after HSCT
timeFrame: 32 months
measure: Distribution of infectious and non-infectious CNS disorders after HSCT
timeFrame: 32 months
measure: Impact of development of CNS disorders on overall survival
timeFrame: 32 months
measure: Risk factors for CNS disorders after allogeneic and autologous HSCT using a prospectively assessed matched control group
timeFrame: 32 months
measure: Efficacy of treatment for different types of CNS disorders
timeFrame: 32 months
Other outcomes:
Locations:
city: Westmead
country: Australia
facility: The Children's Hospital at Westmead
city: Medellín
country: Colombia
facility: Instituto de Cancerologia S.A
city: Paris
country: France
facility: Hopital St. Louis
city: Cottbus
country: Germany
facility: Carl-Thiem-Klinikum
city: Jena
country: Germany
facility: Jena University Hospital
city: Wuerzburg
country: Germany
facility: University Children's Hospital
city: Würzburg
country: Germany
facility: University Children's Hospital
city: Budapest
country: Hungary
facility: Central Hospital of Southern Pest
city: Jarusalem
country: Israel
facility: Hadassah University Hospital
city: Genova
country: Italy
facility: Institute G. Gaslini
city: Genova
country: Italy
facility: Ospedale San Martino
city: Padova
country: Italy
facility: Clinica di Oncoematologia Pediatrica
city: Rome
country: Italy
facility: Universita Cattolica S. Cuore
city: Bydgoszcz
country: Poland
facility: University Hospital, Collegium Medicum UMK
city: Kraków
country: Poland
facility: University Children's Hospital in Krakow
city: Warsaw
country: Poland
facility: The Medical University of Warsaw
city: St. Petersburg
country: Russian Federation
facility: Raisa Gorbacheva Research Institute for Oncology
city: Barcelona
country: Spain
facility: Hospital Santa Creu i Sant Pau
city: Madrid
country: Spain
facility: Hospital Univ. 12 de Octubre
city: Madrid
country: Spain
facility: Niño Jesus Children's Hospital
city: Valencia
country: Spain
facility: University Hospital La Fe
city: Tunis
country: Tunisia
facility: Centre Ntl de greffe de MO de Tunis
city: Antalya
country: Turkey
facility: Hacettepe University Children's Hospital
Study officials:
affiliation: Carl-Thiem-Klinikum, Clinic for Hematology and Oncology, Cottbus, Germany
name: Martin Schmidt-Hieber, Dr
role: PRINCIPAL_INVESTIGATOR
References: