Clinical trial NCT00390637: Diet, Obesity and Genes (DiOGenes)
Official title: Diet, Obesity and Genes
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: PHASE4
Conditions: Obesity
Metabolic Syndrome
Type 2 Diabetes
Cardiovascular Disease
Keywords: Diogenes
Diet Intervention
Family
Glycemic Index
Protein
Lead sponsor: University of Copenhagen
Sponsor class: OTHER
Collaborators: name: European Commission
First posted: 2006-10-20
Enrollment: 1020
Sex: ALL
Minimum age (years): 5
Maximum age (years): 65
Standard ages: CHILD
ADULT
OLDER_ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: PARALLEL
Primary purpose: PREVENTION

Brief summary: Study Title: Diogenes, A randomised controlled trial comparing the effect of reduced-fat diets varying in glycaemic index (high vs. low) and protein content (high vs. normal) on bodyweight in overweight and obese subjects after an initial weight loss.

Detailed description: DiOGenes is a randomised controlled dietary intervention study in obese/overweight families (adults and children) in 8 different European countries, testing the efficacy and safety of diets differing in Glycaemic Index (GI) and protein content.

The 8 centres are divided into "shop centers" and "instruction centers", that provide different kind of dietary intervention and for a different length(see below):

The 2 Shop Centers (Denmark \& The Netherlands):

Dietary periods and clinical examinations:

1. Clinical examination 1 with anthropometry, blood etc.
2. 8 week Low Calorie Diet (LCD) period (800-1000 kcal/day)(Adults only)
3. Clinical examination 2 with anthropometry, blood etc.
4. 6 months dietary intervention period 1 (SUPERMARKET period)(Family)
5. Clinical examination 3 with anthropometry, blood etc.
6. 6 months dietary intervention period 2 (DIETARY INSTRUCTION period)(Family).
7. Clinical examination 4 with anthropometry, blood ect.
8. 12 months free-living period with no dietary intervention. (Family)
9. Follow up (only body weight registration)

The 6 Instruction Centers (UK, Crete, Germany, Spain, Bulgaria, Czech Republic)

Dietary periods and clinical examinations:

1. Clinical examination 1 with anthropometry, blood etc.
2. 8 week Low Calorie Diet (LCD) period (800-1000 kcal/day). (Adults only)
3. Clinical examination 2 with anthropometry, blood etc.
4. 6 months dietary intervention period (DIETARY INSTRUCTION period). (Family)
5. Clinical examination 3 with anthropometry, blood etc.
6. 6 months free-living period with no dietary intervention. (Family)
7. Follow up (only body weight registration)

Note that, during the first 6 month intervention all families from the shop centers are provided all foods free of charge "SUPERMARKET PERIOD", whereas in the "Instruction Centers", the families receive dietary instruction only "Instruction Only".

Eligibility criteria: Inclusion Criteria:

* Families with healthy adults and children

Exclusion Criteria:

* Various

Primary outcomes:
measure: For adults: body weight loss maintenance
timeFrame: 6 Month
Secondary outcomes:
measure: For adults: change in body composition
timeFrame: 6 Month
measure: For adults: More than 5% weight loss maintenance
timeFrame: 6 month
measure: For adults: More than 10% weight loss maintenance
timeFrame: 6 month
measure: For adults: Drop out rate
timeFrame: 6 month
measure: For Adults: Change in risk factors for type-2 diabetes
timeFrame: 6 month
measure: For Adults: Change in risk factors for cardiovascular disease
timeFrame: 6 month
measure: For children: Change in body weight
timeFrame: 6 month
measure: For Children: Change in BMI-Z-score
timeFrame: 6 month
Other outcomes:
measure: For adults: Identify traits biological that determine responses to the dietary intervention.
timeFrame: 12 months
measure: For adults: Identify psychological traits that determine responses to the dietary intervention.
timeFrame: 12 months
measure: For adults: Identify traits gene - diet interactions that determine responses to the dietary intervention.
timeFrame: 12 months
Locations:
city: Copenhagen
country: Denmark
facility: Arne V. Astrup
Study officials:
affiliation: Institute of Human Nutrition, RVAU, Denmark
name: Arne V Astrup, Professor
role: PRINCIPAL_INVESTIGATOR
References:
citation: Saris WH, Harper A. DiOGenes: a multidisciplinary offensive focused on the obesity epidemic. Obes Rev. 2005 May;6(2):175-6. doi: 10.1111/j.1467-789x.2005.00200.x. No abstract available.
citation: Vidal-Ostos F, Ramos-Lopez O, Jebb SA, Papadaki A, Pfeiffer AFH, Handjieva-Darlenska T, Kunesova M, Blaak EE, Astrup A, Martinez JA; Diet, Obesity, and Genes (Diogenes) Project. Dietary protein and the glycemic index handle insulin resistance within a nutritional program for avoiding weight regain after energy-restricted induced weight loss. Nutr Metab (Lond). 2022 Oct 19;19(1):71. doi: 10.1186/s12986-022-00707-y.
citation: Imbert A, Vialaneix N, Marquis J, Vion J, Charpagne A, Metairon S, Laurens C, Moro C, Boulet N, Walter O, Lefebvre G, Hager J, Langin D, Saris WHM, Astrup A, Viguerie N, Valsesia A. Network Analyses Reveal Negative Link Between Changes in Adipose Tissue GDF15 and BMI During Dietary-induced Weight Loss. J Clin Endocrinol Metab. 2022 Jan 1;107(1):e130-e142. doi: 10.1210/clinem/dgab621.
citation: van der Kolk BW, Muniandy M, Kaminska D, Alvarez M, Ko A, Miao Z, Valsesia A, Langin D, Vaittinen M, Paakkonen M, Jokinen R, Kaye S, Heinonen S, Virtanen KA, Andersson DP, Mannisto V, Saris WH, Astrup A, Ryden M, Blaak EE, Pajukanta P, Pihlajamaki J, Pietilainen KH. Differential Mitochondrial Gene Expression in Adipose Tissue Following Weight Loss Induced by Diet or Bariatric Surgery. J Clin Endocrinol Metab. 2021 Apr 23;106(5):1312-1324. doi: 10.1210/clinem/dgab072.
citation: Ruffieux H, Carayol J, Popescu R, Harper ME, Dent R, Saris WHM, Astrup A, Hager J, Davison AC, Valsesia A. A fully joint Bayesian quantitative trait locus mapping of human protein abundance in plasma. PLoS Comput Biol. 2020 Jun 3;16(6):e1007882. doi: 10.1371/journal.pcbi.1007882. eCollection 2020 Jun.
citation: Turicchi J, O'Driscoll R, Finlayson G, Duarte C, Hopkins M, Martins N, Michalowska J, Larsen TM, van Baak MA, Astrup A, Stubbs RJ. Associations between the proportion of fat-free mass loss during weight loss, changes in appetite, and subsequent weight change: results from a randomized 2-stage dietary intervention trial. Am J Clin Nutr. 2020 Mar 1;111(3):536-544. doi: 10.1093/ajcn/nqz331.
citation: van der Kolk BW, Kalafati M, Adriaens M, van Greevenbroek MMJ, Vogelzangs N, Saris WHM, Astrup A, Valsesia A, Langin D, van der Kallen CJH, Eussen SJPM, Schalkwijk CG, Stehouwer CDA, Goossens GH, Arts ICW, Jocken JWE, Evelo CT, Blaak EE. Subcutaneous Adipose Tissue and Systemic Inflammation Are Associated With Peripheral but Not Hepatic Insulin Resistance in Humans. Diabetes. 2019 Dec;68(12):2247-2258. doi: 10.2337/db19-0560. Epub 2019 Sep 6.
citation: Valsesia A, Kulkarni SS, Marquis J, Leone P, Mironova P, Walter O, Hjorth MF, Descombes P, Hager J, Saris WH, Astrup A, Darimont C, O'Callaghan NJ. Salivary alpha-amylase copy number is not associated with weight trajectories and glycemic improvements following clinical weight loss: results from a 2-phase dietary intervention study. Am J Clin Nutr. 2019 Apr 1;109(4):1029-1037. doi: 10.1093/ajcn/nqy363.
citation: Svendstrup M, Allin KH, Angquist L, Schnohr P, Jensen GB, Linneberg A, Thuesen B, Astrup A, Saris WHM, Vestergaard H, Sorensen TIA. Is abdominal obesity at baseline influencing weight changes in observational studies and during weight loss interventions? Am J Clin Nutr. 2018 Nov 1;108(5):913-921. doi: 10.1093/ajcn/nqy187.
citation: Meyer A, Montastier E, Hager J, Saris WHM, Astrup A, Viguerie N, Valsesia A. Plasma metabolites and lipids predict insulin sensitivity improvement in obese, nondiabetic individuals after a 2-phase dietary intervention. Am J Clin Nutr. 2018 Jul 1;108(1):13-23. doi: 10.1093/ajcn/nqy087.
citation: Armenise C, Lefebvre G, Carayol J, Bonnel S, Bolton J, Di Cara A, Gheldof N, Descombes P, Langin D, Saris WH, Astrup A, Hager J, Viguerie N, Valsesia A. Transcriptome profiling from adipose tissue during a low-calorie diet reveals predictors of weight and glycemic outcomes in obese, nondiabetic subjects. Am J Clin Nutr. 2017 Sep;106(3):736-746. doi: 10.3945/ajcn.117.156216. Epub 2017 Aug 9.
citation: Hjorth MF, Ritz C, Blaak EE, Saris WH, Langin D, Poulsen SK, Larsen TM, Sorensen TI, Zohar Y, Astrup A. Pretreatment fasting plasma glucose and insulin modify dietary weight loss success: results from 3 randomized clinical trials. Am J Clin Nutr. 2017 Aug;106(2):499-505. doi: 10.3945/ajcn.117.155200. Epub 2017 Jul 5.
citation: Bolton J, Montastier E, Carayol J, Bonnel S, Mir L, Marques MA, Astrup A, Saris W, Iacovoni J, Villa-Vialaneix N, Valsesia A, Langin D, Viguerie N. Molecular Biomarkers for Weight Control in Obese Individuals Subjected to a Multiphase Dietary Intervention. J Clin Endocrinol Metab. 2017 Aug 1;102(8):2751-2761. doi: 10.1210/jc.2016-3997.
citation: Oller Moreno S, Cominetti O, Nunez Galindo A, Irincheeva I, Corthesy J, Astrup A, Saris WHM, Hager J, Kussmann M, Dayon L. The differential plasma proteome of obese and overweight individuals undergoing a nutritional weight loss and maintenance intervention. Proteomics Clin Appl. 2018 Jan;12(1). doi: 10.1002/prca.201600150. Epub 2017 May 15.
citation: Valsesia A, Saris WH, Astrup A, Hager J, Masoodi M. Distinct lipid profiles predict improved glycemic control in obese, nondiabetic patients after a low-caloric diet intervention: the Diet, Obesity and Genes randomized trial. Am J Clin Nutr. 2016 Sep;104(3):566-75. doi: 10.3945/ajcn.116.137646. Epub 2016 Aug 10.
citation: Navas-Carretero S, Holst C, Saris WH, van Baak MA, Jebb SA, Kafatos A, Papadaki A, Pfeiffer AF, Handjieva-Darlenska T, Hlavaty P, Stender S, Larsen TM, Astrup A, Martinez JA. The Impact of Gender and Protein Intake on the Success of Weight Maintenance and Associated Cardiovascular Risk Benefits, Independent of the Mode of Food Provision: The DiOGenes Randomized Trial. J Am Coll Nutr. 2016;35(1):20-30. doi: 10.1080/07315724.2014.948642. Epub 2015 Mar 31.
citation: Montastier E, Dejean S, Le Gall C, Saris WH, Langin D, Viguerie N. Adipose tissue CIDEA is associated, independently of weight variation, to change in insulin resistance during a longitudinal weight control dietary program in obese individuals. PLoS One. 2014 Jul 1;9(7):e98707. doi: 10.1371/journal.pone.0098707. eCollection 2014.
citation: Bendtsen LQ, Lorenzen JK, Larsen TM, van Baak M, Papadaki A, Martinez JA, Handjieva-Darlenska T, Jebb SA, Kunesova M, Pfeiffer AF, Saris WH, Astrup A, Raben A. Associations between dairy protein intake and body weight and risk markers of diabetes and CVD during weight maintenance. Br J Nutr. 2014 Mar 14;111(5):944-53. doi: 10.1017/S0007114513003322. Epub 2013 Oct 30.
citation: Damsgaard CT, Papadaki A, Jensen SM, Ritz C, Dalskov SM, Hlavaty P, Saris WH, Martinez JA, Handjieva-Darlenska T, Andersen MR, Stender S, Larsen TM, Astrup A, Molgaard C, Michaelsen KF; Diogenes. Higher protein diets consumed ad libitum improve cardiovascular risk markers in children of overweight parents from eight European countries. J Nutr. 2013 Jun;143(6):810-7. doi: 10.3945/jn.112.173427. Epub 2013 Apr 17.
citation: Papadaki A, Linardakis M, Plada M, Larsen TM, van Baak MA, Lindroos AK, Pfeiffer AF, Martinez JA, Handjieva-Darlenska T, Kunesova M, Holst C, Saris WH, Astrup A, Kafatos A; Diet, Obesity and Genes (DiOGenes) Project. A multicentre weight loss study using a low-calorie diet over 8 weeks: regional differences in efficacy across eight European cities. Swiss Med Wkly. 2013 Jan 21;143:w13721. doi: 10.4414/smw.2013.13721. eCollection 2013.
citation: Larsen LH, Angquist L, Vimaleswaran KS, Hager J, Viguerie N, Loos RJ, Handjieva-Darlenska T, Jebb SA, Kunesova M, Larsen TM, Martinez JA, Papadaki A, Pfeiffer AF, van Baak MA, Sorensen TIa, Holst C, Langin D, Astrup A, Saris WH. Analyses of single nucleotide polymorphisms in selected nutrient-sensitive genes in weight-regain prevention: the DIOGENES study. Am J Clin Nutr. 2012 May;95(5):1254-60. doi: 10.3945/ajcn.111.016543. Epub 2012 Apr 4.
citation: Gogebakan O, Kohl A, Osterhoff MA, van Baak MA, Jebb SA, Papadaki A, Martinez JA, Handjieva-Darlenska T, Hlavaty P, Weickert MO, Holst C, Saris WH, Astrup A, Pfeiffer AF; DiOGenes. Effects of weight loss and long-term weight maintenance with diets varying in protein and glycemic index on cardiovascular risk factors: the diet, obesity, and genes (DiOGenes) study: a randomized, controlled trial. Circulation. 2011 Dec 20;124(25):2829-38. doi: 10.1161/CIRCULATIONAHA.111.033274. Epub 2011 Nov 21.
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citation: Rubio-Aliaga I, Marvin-Guy LF, Wang P, Wagniere S, Mansourian R, Fuerholz A, Saris WH, Astrup A, Mariman EC, Kussmann M. Mechanisms of weight maintenance under high- and low-protein, low-glycaemic index diets. Mol Nutr Food Res. 2011 Nov;55(11):1603-12. doi: 10.1002/mnfr.201100081. Epub 2011 Sep 29.
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