Clinical trial NCT02801344: The Impact of Protein Intake on Protein Metabolism During Intensified Training
Official title: The Impact of Protein Intake on Amino Acid and Protein Metabolism, and Performance During Intensified Training
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Healthy
Keywords: exercise
performance
recovery
muscle
protein
Lead sponsor: University of Toronto
Sponsor class: OTHER
Collaborators: name: Ajinomoto Co., Inc.
First posted: 2016-06-15
Enrollment: 12
Sex: MALE
Minimum age (years): 18
Maximum age (years): 50
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: CROSSOVER
Primary purpose: BASIC_SCIENCE

Brief summary: Protein requirements in individuals who participate in endurance-based exercise training have been suggested to be greater than the current recommended dietary allowance (RDA).

Our recent study using the minimally invasive indicator amino acid oxidation (IAAO) technique have suggested that protein requirements in young men are at least 30% higher than the recommended protein intake.

The present study will investigate the impact of protein sufficiency on protein metabolism and performance during intensified training periods as a means to further our understanding of the nutritional requirements for the endurance athlete.


Eligibility criteria: Inclusion Criteria:

* Healthy, male, endurance-trained participants who have run regularly more than 45 km or 4.5 hours/week
* Participants who are categorized at least "very good" based on a study by Shvartz \& Reibold \[40\], in which peak rate of oxygen consumption(VO2 Peak) is used as an index. (i.e. the participants whose VO2peak is ≥57 ml/kg/min (18-24 y), ≥54 ml/kg/min (25-29 y), ≥52 ml/kg/min (30-34 y), ≥49 ml/kg/min (35-39 y), ≥47 ml/kg/min (40-44 y), ≥44 ml/kg/min (45-50 y) according to his age,
* Participants who can cover 10 km in less than 60 min after the VO2peak test and 5 km Time trial on session 2.
* Participants will be 18-50 years old.
* Participants are willing to abide by the compliance rules of this study

Exclusion Criteria:

* Inability to meet health and physical activity guidelines according to the The Physical Activity Readiness Questionnaire for everyone.
* Female
* Inability to adhere to any of the compliance rules judged by principal investigator or medical doctor
* Regular tobacco use
* Illicit drug use (e.g. growth hormone, testosterone, etc.)

Primary outcomes:
measure: Change in 24h whole body protein balance
timeFrame: Difference between day 1 and day 4 whole body protein balance
Secondary outcomes:
measure: Change from baseline physical performance (5 km time trial) 5days after the beginning of training
timeFrame: 5days after the beginning of training
measure: change from baseline Physical performance (muscle strength) 5days after the beginning of training
timeFrame: 5days after the beginning of training
measure: change from baseline Physical Performance (muscle power) 5days after the beginning of training
timeFrame: 5days after the beginning of training
Other outcomes:
measure: Creatine Phosphate Kinase activity on day 5
timeFrame: 5days after the beginning of training
measure: Myoglobin concentration on day 5
timeFrame: 5days after the beginning of training
measure: IL-6 concentration on day 5
timeFrame: 5days after the beginning of training
measure: CRP concentration on day 5
timeFrame: 5days after the beginning of training
measure: amino acid concentration on day 5
timeFrame: 5days after the beginning of training
Locations:
city: Toronto
country: Canada
facility: Goldring Centre For High Performance Sport
state: Ontario
Study officials:
affiliation: Faculty of Kinesiology and Physical Education, University of Toronto
name: Daniel Moore, Ph.D.
role: PRINCIPAL_INVESTIGATOR
References: