Clinical trial NCT05412667: Investigation of TWK10 Administration on the Effects of Amino Acid Absorption
Official title: Investigation of TWK10 Administration on the Effects of Amino Acid Absorption
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Amino Acid
Exercise
Probiotic
Body Composition
Lead sponsor: National Taiwan Sport University
Sponsor class: OTHER
Collaborators: name: Synbio Tech Inc.
First posted: 2022-06-09
Enrollment: 32
Sex: ALL
Minimum age (years): 20
Maximum age (years): 45
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: PARALLEL
Primary purpose: BASIC_SCIENCE

Brief summary: The most common problem among sports people is that no matter how much protein food or products they eat, their sports performance cannot be improved, resulting in failure to achieve breakthroughs in sports performance. The cause of the trouble is digestion and absorption problems. The key is intestinal problems. If gastrointestinal function is maintained in a healthy state, then exercise performance must be maintained at a considerable level. Therefore, how to choose the source of protein in the diet is one of the most concerned issues of the sports crowd. Although past studies have confirmed that the essential amino acids in animal protein can be absorbed and utilized better than plant protein, plant protein can be broken down into easily digestible peptides and amino acids by pepsin. Promote the metabolite pool in the large intestine and the amino acid balance of the host in the small intestine. Recent studies have pointed out that the proteases and peptidases in lactic acid bacteria can provide free amino acids for the best growth of bacteria, and can increase the distribution of amino acids in the blood, the speed of muscle synthesis and the content of branched chain amino acids. However, the mechanism of action of Lactobacillus plantarum on protein digestion and amino acid absorption in the host is still unclear. Therefore, the purpose of this study is to explore the effect of supplementing sports lactic acid bacteria TWK10 in human experiments to effectively improve the amino acid bioabsorption rate of plant protein supplements. 40 subjects were randomly divided into 2 groups, each with 20 people (male and female): (1) pea protein without TWK10 (placebo), (2) TWK10 group (TWK10). The two groups of subjects were supplemented with test samples for 28 consecutive days and performed paired sports training 3 times a week. Before and after the intervention, the samples were subjected to exercise testing, blood and fecal sample collection, body composition analysis, and muscle mass analysis.


Eligibility criteria: Inclusion Criteria:

* Have exercise habits (exercise at least 3 times a week for at least 30 minutes each time),
* Healthy body

Exclusion Criteria:

* Smoking
* Alcohol consumption
* Cardiovascular disease
* Neuromuscular disease
* Metabolic disease
* Asthma, pregnant or breastfeeding
* BMI\>27
* Allergic to peanuts .

Primary outcomes:
measure: Amino acid
timeFrame: From Baseline to 4 Weeks Assessed, including time point: baseline, 30 minutes, 60 minutes, 120 minutes, and 180 minutes post ingestion)
measure: Muscle composition
timeFrame: From Baseline to 4 Weeks Assessed
measure: Isometric Mid-Thigh Pull (IMTP)
timeFrame: From Baseline to 4 Weeks Assessed
measure: Isometric Mid-Thigh Pull (IMTP)
timeFrame: From Baseline to 4 Weeks Assessed
measure: Wingate Anaerobic Test (WAnT)
timeFrame: From Baseline to 4 Weeks Assessed
measure: Wingate Anaerobic Test (WAnT)
timeFrame: From Baseline to 4 Weeks Assessed
measure: Wingate Anaerobic Test (WAnT)
timeFrame: From Baseline to 4 Weeks Assessed
measure: dual-energy X-ray absorptiometry, DXA
timeFrame: From Baseline to 4 Weeks Assessed
measure: dual-energy X-ray absorptiometry, DXA
timeFrame: From Baseline to 4 Weeks Assessed
measure: Bench press test
timeFrame: From Baseline to 4 Weeks Assessed
Secondary outcomes:
measure: Checklist Individual Strength (CIS) questionnaire
timeFrame: From Baseline to 4 Weeks Assessed
measure: Visual Analog Fatigue Scale (VAFS)
timeFrame: From Baseline to 4 Weeks Assessed
measure: Rating of Perceived Exertion (RPE) scale
timeFrame: From Baseline to 4 Weeks Assessed
measure: 3-day dietary recording four weeks
timeFrame: From Baseline to 4 Weeks Assessed
Other outcomes:
Locations:
city: Taoyuan
country: Taiwan
facility: Graduate Institute of Sports Science, National Taiwan Sport University
Study officials:
References: