Clinical trial NCT04321772: Muscle Response to High Intensity Interval Resistance Training (HIIRT)
Official title: Effects of Exercise Intensity and Rest Intervals on Intracellular Signals and Anabolic Response of Skeletal Muscle to Resistance Training
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Exercise
Resistance Training
Keywords: resistance training
recovery
muscle hypertrophy
muscle signalling
Lead sponsor: University of Padova
Sponsor class: OTHER
Collaborators: name: University of Roma La Sapienza
First posted: 2020-03-25
Enrollment: 9
Sex: ALL
Minimum age (years): 20
Maximum age (years): 30
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: CROSSOVER
Intervention model description: Same subjects serve as both the test and control arms which allows for within-subject comparison. Using a within-person design, participants trained each lower limb on separate days under two different training stimulus (HIIRT) and traditional training (TRT)
Primary purpose: BASIC_SCIENCE

Brief summary: Resistance training (RT) is one of the most important stimuli for muscle hypertrophy and it plays an important role on weight loss and fatty acid oxidation increase. RT affects anabolic pathways but the differences among various training techniques has been till now were poorly investigated. The aim of this study was to compare the effect of two different intensity of training, high-intensity interval resistance training (HIIRT) and traditional resistance training (TRT), on muscle signalling pathway.

Detailed description: Nine young healthy subjects performed HIIRT and TRT protocol in two different moments and with different legs. HIIRT technique consisted of three sets of: 6 repetitions at 6RM (6 repetitions maximum) and then 20 seconds of rest and 2/3 repetitions (until exhaustion) repeated for 3 times with 2'30" rest between sets; while TRT consisted of 3 sets of 15 reps with 75 sec of rest between sets. Biopsies from the vastus lateralis were taken at baseline (pre), immediately (0h) at the end of training, 6 hours (6h) and 24 hours (24h) after training. Western blot and RT-PCR mRNA (Reverse transcriptase-polymerase chain reaction) analysis were performed to assess muscle signalling pathway activation

Eligibility criteria: Inclusion Criteria:

* moderate active
* experience with resistance training

Exclusion Criteria:

* history of recent myocardial infarction,
* severe cardiac arrhythmia,
* unstable angina,
* poorly controlled hypertension,
* poorly controlled diabetes mellitus,
* frequent or complex ventricular ectopy which might interfere with one's ability to adhere to exercise protocols

Primary outcomes:
measure: pAMPKThr172 in skeletal muscle tissue measured by immunoblot
timeFrame: 24 hours
measure: pERK 1/2Thr202/Tyr204 in skeletal muscle tissue measured by immunoblot
timeFrame: 24 hours
measure: pS6Ser235/236 in skeletal muscle tissue measured by immunoblot
timeFrame: 24 hours
measure: p4EBP1Thr37/46 in skeletal muscle tissue measured by immunoblot
timeFrame: 24 hours
measure: ACC (acetyl-coenzyme A carboxylase) phosphorylation in skeletal muscle tissue measured by immunoblot
timeFrame: 24 hours
measure: IGF-1 mRNA (messenger ribonucleic acid) in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)
timeFrame: 24 hours
measure: IGF-1ea (insulin like growth factor) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)
timeFrame: 24 hoursn
measure: MGF (mechanical growth factor) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)
timeFrame: 24 hours
measure: STARS mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)
timeFrame: 24 hoursn
measure: Myogenin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)
timeFrame: 24 hours
measure: IL-6 (interleukin 6) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)
timeFrame: 24 hours
measure: PGC1a4 (Peroxisome proliferator-activated receptor gamma coactivator 1-alpha 4) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)
timeFrame: 24 hours
measure: PGC1a (Peroxisome proliferator-activated receptor gamma coactivator 1-alpha) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)
timeFrame: 24 hours
measure: Atrogin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)
timeFrame: 24 hours
measure: Beclin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)
timeFrame: 24 hours
measure: Myostatin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)
timeFrame: 24 hours
Secondary outcomes:
Other outcomes:
Locations:
city: Padova
country: Italy
facility: Nutrition and Exercise Lab, DSB, University of Padova
Study officials:
affiliation: University of Padova
name: Antonio Paoli, MD
role: STUDY_DIRECTOR
References:
citation: Paoli A. Resistance training: the multifaceted side of exercise. Am J Physiol Endocrinol Metab. 2012 Feb 1;302(3):E387. doi: 10.1152/ajpendo.00541.2011. No abstract available.
citation: Paoli A, Moro T, Marcolin G, Neri M, Bianco A, Palma A, Grimaldi K. High-Intensity Interval Resistance Training (HIRT) influences resting energy expenditure and respiratory ratio in non-dieting individuals. J Transl Med. 2012 Nov 24;10:237. doi: 10.1186/1479-5876-10-237.
citation: Paoli A, Pacelli QF, Cancellara P, Toniolo L, Moro T, Canato M, Miotti D, Reggiani C. Myosin isoforms and contractile properties of single fibers of human Latissimus Dorsi muscle. Biomed Res Int. 2013;2013:249398. doi: 10.1155/2013/249398. Epub 2013 Jul 22.