Clinical trial NCT00978471: Adjuvant High-Dose Thiotepa and Stem Cell Rescue Associated With Conventional Chemotherapy in Relapsed Osteosarcoma
Official title: Efficacy and Tolerance Adjuvant High-Dose Thiotepa With Peripheral Stem Cell Rescue Associated With Conventional Chemotherapy in Children and Adults With Relapsed Osteosarcoma
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: PHASE2
Conditions: Osteosarcoma
Keywords: Conventional chemotherapy
cytapheresis
Peripheral stem cell autograft
Surgical resection
Thiotepa
Lead sponsor: Centre Leon Berard
Sponsor class: OTHER
First posted: 2009-09-17
Enrollment: 44
Sex: ALL
Minimum age (years): 1
Maximum age (years): 50
Standard ages: CHILD
ADULT
Accepts healthy volunteers: no
Allocation: RANDOMIZED
Intervention model: PARALLEL
Primary purpose: TREATMENT

Brief summary: Approximately 150 new cases of osteosarcoma are reported each year in France, of which 15 to 20% are metastatic.

Further to the initial standard care, about 45% of the patients relapse within a median duration of 20 months.

Result of the OS94 study results and of the investigation performed within the CRLCC, indicate that 25 to 30 patients (children and adults) experience an osteosarcoma relapse each year in FRANCE.

According to several studies, the 5-year overall survival rate of patients in first relapse is 23-28%,with a median post relapse survival of 10 to 17 months. Multiple relapse cases are also reported in the COSS study, with a median time to second relapse of 0.8 year.

At present, there is no reference treatment for the standard care of osteosarcoma relapse in FRANCE.

Thiotepa is known for its antitumor effect in numerous malignant tumors. In 2007, a study from our institution reported that about 35% of all osteosarcoma relapses are treated with a high-dose thiotepa while the efficacy and tolerance of this therapeutic strategy have never been assessed.

These results highlight the need to the evaluate the efficacy and tolerance of this high-dose of thiotepa within a clinical trial and its inclusion in the standard care of the osteosarcoma at relapse.

Detailed description: Despite the absence of tumor registry, approximately 150 new cases of osteosarcoma are reported each year in France (100 cases per year in children and 50 cases in adults), of which 15 to 20% are metastatic. The standardized impact rate in the world population is estimated at 3 per million inhabitants per year.

Further to the initial standard care, about 45% of the patients relapse within a median interval of 20 months (range 3 months - 10 years).

Results of the OS94 study and of the investigation performed within the CRLCC indicate that 25 to 30 patients (children and adults) experience an osteosarcoma relapse each year in FRANCE.

Results of the five major published series indicate that the 5-year overall survival rate of patients in first relapse is between 23 and 28%, with a median post-relapse survival of 10 to 17 months. Multiple relapse cases are also reported in the COSS study, with a median time to second relapse of 0.8 year.

At present, there is no reference treatment for the standard care of osteosarcoma relapse in FRANCE.

Some recommendations have been given in the OS94 protocol, but they are generally not followed or they are implemented in a heterogeneous manner.

Thiotepa (N N' N'' triethylenethiophosphoramide), an alkylating agent of the chemical family of ethylene-imines, is known for its antitumor effect in a number of malignant tumors.

Its efficacy in osteosarcoma has been reported in the literature. A retrospective study of the SFCE (French Society for Childhood Cancer, results not yet published) in 45 patients presenting with refractory osteosarcoma or relapse has shown a radiological reaction rate of 30%.

Moreover, a preliminary investigation performed by the CLB in 2007 within the framework of the SFCE study explored all relapse cases diagnosed between the beginning of 2004 and the end of 2006. Results showed that about 35% of the patients with osteosarcoma relapses are treated with high-dose thiotepa while the efficacy and tolerance of this therapeutic strategy have never been assessed.

Altogether, these results led the SFCE osteosarcoma group to propose the evaluation of the efficacy and tolerance of this high-dose thiotepa chemotherapy within a clinical trial and to include the drug in the standard care of osteosarcoma in relapse.

Eligibility criteria: Inclusion Criteria:

* Age \> 1 year and \< 50 years
* First osteosarcoma relapse, either local or metastatic, or second relapse after exclusive surgery NB: Whenever possible, only patients with histological evidence of relapse will be included.
* Indication for chemotherapy confirmed by a multidisciplinary committee.
* Surgical resection of all tumor sites must be possible, either as first-line therapy or after chemotherapy.
* Lansky score ≥ 60%, or ECOG Performance Status ≤ 2
* ≥ 21-day interval after first-line chemotherapy
* Blood tests, renal and liver functions within the normal range for age with, in particular, 7 days prior to study entry, blood or serum values as follows:
* blood: neutrophil count \> 1 G/L; platelets \>100 G/L
* renal: serum creatinine ≤ 1.5 x ULN depending on age; patients with serum creatinine values \> 1.5 x ULN are eligible if creatinine clearance is \> 70 mL/min/1.73 m²
* liver: total bilirubin \< 2 x ULN; ASAT and ALAT ≤ 5 x ULN
* cardiac: isotopic or echographic Left Ventricular Ejection Fraction \> 50 %.
* Signed written informed consent; for children, signed consent from the patient (depending on age) and from the parents or legal representative is mandatory
* Documented negative serum βHCG for female patients of childbearing age
* Affiliation with health insurance.

Exclusion Criteria:

* Patients with multiple relapses for whom surgical resection seems impossible, even after chemotherapy.
* Patients already treated with high-dose chemotherapy regimens
* Patients with a contra-indication to the treatment proposed
* Patients not eligible for leukapheresis
* Two-year follow-up impossible due to social, family, geographic or psychological reasons
* Patient included in another protocol of clinical research
* Pregnant or lactating women.

Primary outcomes:
measure: Estimate the overall survival rate
timeFrame: 24 months
Secondary outcomes:
measure: Estimate overall survival after relapse diagnosis
timeFrame: 24 months
measure: Estimate the survival free progression after randomization
timeFrame: 24 months
measure: Evaluate the tolerance profile of experimental treatment (hematologic toxicity)
timeFrame: every 3 weeks
measure: Estimate the rate of tumor response to treatment as assessed by conventional CT-scan
timeFrame: at inclusion, day 14-day 21 after the 2nd chemotherapy cycle, before randomization, day 14-day 21 after the 4th chemotherapy cycle, after thiotepa cure and 8 weeks after the end of treatment
measure: Estimate histological response to treatment on surgical tumor samples
timeFrame: If surgery is applicable, a few weeks after thiotepa cure (12 to 17 weeks after inclusion)
measure: Study of biological and genomic properties and analysis of angiogenic markers correlated with relapse (optional)
timeFrame: At inclusion,at surgery , and at the end of treatment
Other outcomes:
Locations:
city: Besancon
country: France
facility: CHU Besançon- Hôpital Jean Minjoz
city: Bordeaux
country: France
facility: Chu - Hopital Des Enfants Bordeaux
city: Dijon
country: France
facility: CHU Dijon Le Bocage, Hôpital d'Enfants
city: Grenoble
country: France
facility: Chu Grenoble
city: Lille
country: France
facility: Centre Oscar Lambret
city: LYON Cedex 08
country: France
facility: Centre Léon Bérard - Institut d'Hémato-Oncologie Pediatrique
city: Marseille
country: France
facility: Institut Paoli Calmettes
city: Marseille
country: France
facility: Hôpital des Enfants de la Timone
city: Nantes
country: France
facility: Chu Nantes - Hopital Meres Et Enfants
city: Nice
country: France
facility: Centre Antoine Lacassagne
city: Nice
country: France
facility: CHU Nice, Hôpital L'Archet 2
city: Paris
country: France
facility: Institut Curie
city: Paris
country: France
facility: Hopital D'Enfants Armand Trousseau
city: Poitiers
country: France
facility: CHU Poitiers, site de la Milétrie
city: Rennes
country: France
facility: CHU RENNES - Hôpital Sud
city: Saint Priest en Jarez
country: France
facility: CHU de SAINT-ETIENNE, Hôpital Nord
city: Saint-denis
country: France
facility: Chu La Reunion
city: Saint-herblain
country: France
facility: Institut de Cancérologie de l'Ouest - René Gauducheau
city: Saint-Priest-en -Jarez
country: France
facility: Institut Lucien Neurwith
city: Strasbourg
country: France
facility: Hopital de Hautepierre
city: Toulouse
country: France
facility: Chu Toulouse - Hopital D'Enfants
city: Vandoeuvre Les Nancy
country: France
facility: Chu Nancy - Hopital D'Enfants
city: Villejuif
country: France
facility: Institut Gustave Roussy
Study officials:
affiliation: Institut d'Hématologie et d'Oncologie Pédiatrique (IHOP) - CLB
name: Perrine MAREC-BÉRARD, Dr
role: PRINCIPAL_INVESTIGATOR
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