Clinical trial NCT00905892: Metabolic Fate Modifications of Saturated Fats After an Overfeeding
Official title: Metabolic Fate Modifications of Saturated Fats After an Overfeeding
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Obesity
Lipid Metabolism
Inflammation
Keywords: overfeeding
stable isotopes
lipoprotein metabolism
lipid oxidation
fat distribution
gene expression
endotoxemia
mass spectrometry/gas chromatography
Lead sponsor: Centre de Recherche en Nutrition Humaine Rhone-Alpe
Sponsor class: OTHER
Collaborators: name: Institut National de la Santé Et de la Recherche Médicale, France
First posted: 2009-05-21
Enrollment: 40
Sex: MALE
Minimum age (years): 18
Maximum age (years): 55
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: NA
Intervention model: SINGLE_GROUP
Primary purpose: BASIC_SCIENCE

Brief summary: The purpose of this study is to determine the partitioning of exogenous lipids in the postprandial period while a study of overfeeding. The method is based on the incorporation of a stable isotopic tracer (d31_palmitic acid, d31_C16) in lipoprotein triglycerides (TG-CHYLOMICRON and TG-VLDL) and in free fatty acids (FFA).

Detailed description: Healthy overweight and lean young men are subjected to an overfeeding during 56 days which corresponds to a supplement of 761 Kcal/day. During two exploration days (before : D0 et after : D56 overfeeding) they have ingested a breakfast with tracer (d31_palmitic acid, d31_C16, 20mg/kg) and blood and urine samples were collected every hour of each exploration day. The enrichment in deuterium was measured by gas chromatography-organic mass spectrometry (GC-OMS) in palmitic acid pool of lipid fractions.

Eligibility criteria: Inclusion Criteria:

* 18 to 55 years old
* BMI 18 to 30 kg/m²
* Stable physical activity
* Safety subject during medical consultation

Exclusion Criteria:

* Medical or surgical history which may affect energy expenditure (renal -cardiovascular -hepatic- endocrine-inflammatory diseases)
* Drug use that could affect energy expenditure (steroids, nicotine substitutes, thyroid hormones)
* Eating disorder
* Intensive sportive activity
* Subjects who Smoke
* Claustrophobic subjects

Primary outcomes:
measure: Post prandial partitioning of exogenous lipids
timeFrame: Before and after overfeeding
Secondary outcomes:
measure: Genes expression in adipose and muscle tissues
timeFrame: Before and after overfeeding
Other outcomes:
Locations:
city: Pierre-Bénite
country: France
facility: Centre de recherche en nutrition humaine Rhone-Alpes
Study officials:
affiliation: Centre de Recherche en Nutrition Humaine Rhône-Alpes
name: Martine Laville, PhD, MD
role: STUDY_DIRECTOR
References:
citation: Segrestin B, Moreno-Navarrete JM, Seyssel K, Alligier M, Meugnier E, Nazare JA, Vidal H, Fernandez-Real JM, Laville M. Adipose Tissue Expansion by Overfeeding Healthy Men Alters Iron Gene Expression. J Clin Endocrinol Metab. 2019 Mar 1;104(3):688-696. doi: 10.1210/jc.2018-01169.
citation: Seyssel K, Alligier M, Meugnier E, Chanseaume E, Loizon E, Canto C, Disse E, Lambert-Porcheron S, Brozek J, Blond E, Rieusset J, Morio B, Laville M, Vidal H. Regulation of energy metabolism and mitochondrial function in skeletal muscle during lipid overfeeding in healthy men. J Clin Endocrinol Metab. 2014 Jul;99(7):E1254-62. doi: 10.1210/jc.2013-4379. Epub 2014 Mar 31.
citation: Alligier M, Meugnier E, Debard C, Lambert-Porcheron S, Chanseaume E, Sothier M, Loizon E, Hssain AA, Brozek J, Scoazec JY, Morio B, Vidal H, Laville M. Subcutaneous adipose tissue remodeling during the initial phase of weight gain induced by overfeeding in humans. J Clin Endocrinol Metab. 2012 Feb;97(2):E183-92. doi: 10.1210/jc.2011-2314. Epub 2011 Dec 7.