Clinical trial NCT04573192: A Study to Evaluate Safety and Efficacy of L19TNF Plus Lomustine in Patients With Glioblastoma at First Progression
Official title: A Study to Evaluate the Safety and Efficacy of the Tumor-targeting Human Antibody-cytokine Fusion Protein L19TNF Plus Lomustine in Patients With Glioblastoma at First Progression
Study type: INTERVENTIONAL
Overall status: RECRUITING
Phase: PHASE1
PHASE2
Conditions: Glioblastoma
Lead sponsor: Philogen S.p.A.
Sponsor class: INDUSTRY
First posted: 2020-10-05
Enrollment: 142
Sex: ALL
Minimum age (years): 18
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: no
Allocation: RANDOMIZED
Intervention model: PARALLEL
Intervention model description: Open label phase I/II study in subjects with glioblastoma at first progression.
Primary purpose: TREATMENT

Brief summary: Glioblastomas are the most common and most aggressive primary brain tumors in adults. The prognosis is poor despite multimodal therapy with surgery, radiotherapy and chemotherapy. Therefore, novel treatments are urgently needed.

L19TNF is a fully human fusion protein consisting of human tumor necrosis factor (TNF)-α fused to the L19 antibody in scFv format, specific to the extra-domain B of fibronectin. TNF not only induces apoptosis or necrosis in certain target cells, but also exerts inflammation and immunity. L19TNF selectively delivers TNF to the tumor site to spare normal tissues from undesired toxicity. Preclinical experiments with L19TNF have demonstrated tumor growth retardation in various mouse tumor models including models of glioma.


Eligibility criteria: Inclusion Criteria:

1. Male or female, age ≥18.
2. Patients with histologically confirmed glioblastoma at unequivocal first recurrence or progression according to RANO criteria.
3. MGMT promotor methylation status known
4. IDH wildtype.
5. Patients may have undergone surgery for recurrence.
6. For operated patients: The histological report must document glioblastoma recurrence and a new MRI will need to be done at 3-5 weeks after surgery (directly before study treatment start). Study treatment will need to start minimum 4 weeks to maximum 6 weeks after surgery.
7. Karnofsky Performance Status (KPS) ≥ 70%.
8. Documented negative test for HIV-HBV-HCV. For HBV serology, the determination of HBsAg and anti-HBcAg Ab is required. In patients with serology documenting previous exposure to HBV, negative serum HBV-DNA is required. For HCV, HCV-RNA or HCV antibody test is required. Subjects with a positive test for HCV antibody but no detection of HCV-RNA indicating no current infection are eligible.
9. Female patients: female patients must be either documented not Women Of Childbearing Potential (WOCBP)\* or must have a negative pregnancy test within 14 days of starting treatment. Additionally WOCBP must agree to use, from the screening to 6 months following the last study drug administration, highly effective contraception methods, as defined by the "Recommendations for contraception and pregnancy testing in clinical trials" issued by the Head of Medicine Agencies' Clinical Trial Facilitation Group (www.hma.eu/ctfg.html) and which include, for instance, progesterone-only or combined (estrogen- and progesterone-containing) hormonal contraception associated with inhibition of ovulation, intrauterine devices, intrauterine hormone-releasing systems, bilateral tubal occlusion or vasectomized partner.

   Male patients: male subjects able to father children must agree to use two acceptable methods of contraception throughout the study (e.g. condom with spermicidal gel). Double-barrier contraception is required.
10. Personally signed and dated informed consent document indicating that the subject has been informed of all pertinent aspects of the study.
11. Willingness and ability to comply with the scheduled visits, treatment plan, laboratory tests and other study procedures.

    * Women of childbearing potential are defined as females who have experienced menarche, are not postmenopausal (12 months with no menses without an alternative medical cause) and are not permanently sterilized (e.g., tubal occlusion, hysterectomy, bilateral oophorectomy, or bilateral salpingectomy).

Exclusion Criteria:

1. Prior treatment for glioblastoma at recurrence, except surgery.
2. Surgical resection or biopsy of glioma within 4 weeks of the start of study treatment.
3. Inability to undergo contrast-enhanced MRI.
4. Prior treatment with lomustine.
5. Known history of allergy to TNF or lomustine, any excipient in the study medication or any other intravenously administered human proteins/peptides/antibodies.
6. Absolute neutrophil count (ANC) \< 1.5 x 10\^9/L; platelets \< 100 x 10\^9/L or haemoglobin (Hb) \< 9.0 g/dl.
7. Chronically impaired renal function as indicated by creatinine clearance \< 60 mL/min or serum creatinine \> 1.5 ULN.
8. Inadequate liver function (ALT, AST, ALP ≥ 2.5 x ULN or total bilirubin ≥ 2.0 x ULN).
9. INR \> 1.5 ULN.
10. Any severe concomitant condition which makes it undesirable for the patient to participate in the study or which could jeopardize compliance with the protocol, in the opinion of the investigator.
11. Active or history of autoimmune disease that might deteriorate when receiving an immuno-stimulatory agent, in the judgement of the investigator.
12. History within the last year of cerebrovascular disease and/or acute or subacute coronary syndromes including myocardial infarction, unstable or severe stable angina pectoris.
13. Heart insufficiency (\> Grade II, New York Heart Association (NYHA) criteria).
14. Clinically significant cardiac arrhythmias or requiring permanent medication.
15. LVEF \<55% or any other abnormalities observed during baseline ECG and echocardiogram investigations that are considered as clinically significant by the investigator. Subjects with current or a history of QT/QTc prolongation are excluded.
16. Uncontrolled hypertension.
17. Known arterial aneurism at high risk of rupture.
18. Ischemic peripheral vascular disease (Grade IIb-IV according to Leriche-Fontaine classification).
19. Medically documented history of or active major depressive episode, bipolar disorder (I or II), obsessive-compulsive disorder, schizophrenia, a history of suicidal attempt or ideation, or homicidal ideation (e.g. risk of doing harm to self or others), or patients with active severe personality disorders.
20. Anxiety ≥ CTCAE Grade 3.
21. Severe diabetic retinopathy such as severe non-proliferative retinopathy and proliferative retinopathy.
22. Major trauma including major surgery (such as abdominal/cardiac/thoracic surgery) within 4 weeks of administration of study treatment.
23. Known history of tuberculosis.
24. Pregnancy or breast feeding.
25. Requirement of chronic administration of high dose corticosteroids or other immunosuppressant drugs. Subjects must have been either off corticosteroids, or on a stable or decreasing dose ≤ 4 mg daily dexamethasone (or equivalent) for 7 days prior to start of treatment. Limited or occasional use of corticosteroids to treat or prevent acute adverse reactions is not considered an exclusion criterion.
26. Presence of active and uncontrolled infections or other severe concurrent disease, which, in the opinion of the investigator, would place the patient at undue risk or interfere with the study.
27. Concurrent malignancies unless the patient has been disease-free without intervention for at least 2 years.
28. Growth factors or immunomodulatory agents within 7 days prior to the administration of study treatment.
29. Serious, non-healing wound, ulcer, or bone fracture.
30. Anticoagulation therapy with P2Y12 antagonists (e.g., clopidogrel, ticagrelor) and vitamin K antagonists (e.g., phenprocoumon, warfarin).
31. Requirement of concurrent use of other anti-cancer treatments or agents other than study medication.
32. Any live vaccination within 4 weeks prior to treatment or plan to receive live vaccination during the study.

Primary outcomes:
measure: For Phase 1: DLT
timeFrame: For Cohort 1 to Cohort 3 from Day 1 to Day 42 after the first administration of lomustine and study drug (Cycle 1)
measure: For Phase 2: Overall Survival
timeFrame: From beginning of treatment to 12 months
Secondary outcomes:
measure: PFS
timeFrame: From the date of enrollment to the date of progression or death for any cause, whichever came first, assessed up to 52 weeks
measure: PFS-rate at 6 months
timeFrame: From the date of enrollment to the date of progression or death for any cause, whichever came first, assessed up to 6 months
measure: OS-rate at 12 months
timeFrame: From beginning of treatment to 12 months
measure: Adverse Events (AE)
timeFrame: Throughout study completion for each patient, a maximum of 52 weeks for each patient
measure: Serious Adverse Events (AE)
timeFrame: Throughout study completion for each patient, a maximum of 52 weeks for each patient
measure: Safety (DILI)
timeFrame: Throughout study completion for each patient, a maximum of 52 weeks for each patient
Other outcomes:
Locations:
city: Bron
country: France
facility: Hôpital Neurologique Pierre Wertheimer
city: Paris
country: France
facility: Hôpital Saint Louis
city: Paris
country: France
facility: Sorbonne University, AP-HP, Paris brain institute
city: Bonn
country: Germany
facility: University Hospital Bonn
city: Köln
country: Germany
facility: University Hospital Köln
city: München
country: Germany
facility: Klinikum rechts der Isar
city: Tübingen
country: Germany
facility: Universitatsklinikum Tubingen
city: Bologna
country: Italy
facility: Azienda USL di Bologna IRCCS delle Scienze Neurologiche di Bologna
city: Milano
country: Italy
facility: Fondazione IRCCS Istituto Neurologico Carlo Besta
city: Padova
country: Italy
facility: Istituto Oncologico Veneto IRCCS
city: Siena
country: Italy
facility: Azienda Ospedaliero-Universitaria Senese Policlinico Le Scotte
city: Torino
country: Italy
facility: AOU Città della Salute e della Scienza di Torino
city: Bern
country: Switzerland
facility: Inselspital Universitätsklinik für Medizinische Onkologie Bern
city: Lausanne
country: Switzerland
facility: Centre Hospitalier Universitaire Vaudois (CHUV)
city: Zürich
country: Switzerland
facility: Universitatspital Zurich - Klinik fur Neurologie & Hirntumorzentrum
Study officials:
References: