Clinical trial NCT05424588: Physical and Cellular Effects of Ultra-cycling
Official title: Effects of a 4400 km Ultra-cycling Non-competitive Race and Related Training on Body Composition and Circulating Progenitors Differentiation
Study type: OBSERVATIONAL
Overall status: COMPLETED
Conditions: Endurance Training
Keywords: ultra-cycling
training
body composition
cell differentiation
Lead sponsor: Azienda Ospedaliera Universitaria Integrata Verona
Sponsor class: OTHER
Collaborators: name: Dr. Michele Braggio
name: Dr.ssa Maria Teresa Valenti
name: Dr.ssa Arianna Minoia
name: Dr. Gianluigi Dorelli
name: Dr. Mattia Cominacini
name: Prof. Francesco Bertoldo
name: Dr.ssa Jessica Bertacco
name: Dr.ssa Tonia De Simone
name: Prof.ssa Maria Grazia Romanelli
name: Prof.ssa Monica Mottes
name: Dr.ssa Lekhana Bhandary
First posted: 2022-06-21
Enrollment: 8
Sex: MALE
Minimum age (years): 18
Maximum age (years): 70
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: yes
Observational model: CASE_ONLY
Time perspective: PROSPECTIVE
Sampling method: NON_PROBABILITY_SAMPLE
Study population: Eight healthy male amateur Caucasian cyclists (47,5±13,5 years) who attended the NC4000 4th edition.

Brief summary: Background: NorthCape4000 (NC4000) is the most participated ultra-endurance cycling race.

Eight healthy male Caucasian amateur cyclists were evaluated: a) before starting the preparation period; b) in the week preceding NC4000 (after the training period); c) after NC4000 race, with the aim to identify the effects of ultra-cycling on body composition, aerobic capacity and biochemical parameters as well as on the differentiation of progenitor cells.

Methods: Bioelectrical impedance analysis (BIA) and dual energy x-ray absorptiometry (DEXA) assessed body composition; cardiopulmonary exercise test (CPET) evaluated aerobic capacity. Differentiation of circulating progenitor cells was evaluated by analyzing the modulation in the expression of relevant transcription factors. In addition, in vitro experiments were performed to investigate the effects of sera of NC4000 participants on adipogenesis and myogenesis. The effects of NC4000 sera on Sestrins and Sirtuin modulation and the promotion of brown adipogenesis in progenitor cells was investigated as well. Two-tailed Student's paired-test was used to perform statistical analyses.

Results: We observed fat mass decrease after training as well as after NC4000 performance; we also recorded that vitamin D and lipid profiles were affected by ultra-cycling. In addition, our findings demonstrated that post-NC4000 participant's pooled sera exerted a positive effect in stimulating myogenesis and in inducing brown adipogenesis in progenitor cells.

Conclusions: The training program and Ultra-cycling lead to beneficial effects on body composition and biochemical lipid parameters, as well as changes in differentiation of progenitor cells , with significant increases in brown adipogenesis and in MYOD levels.


Eligibility criteria: Inclusion Criteria:

* male amateur Caucasian cyclists who attended the NC4000 4th edition

Exclusion Criteria:

* any condition that might have altered performance or laboratory tests results

Primary outcomes:
measure: Body composition
timeFrame: DEC 2020-SEP 2021
measure: Cell differentiation
timeFrame: DEC 2020-SEP 2021
Secondary outcomes:
Other outcomes:
Locations:
city: Verona
country: Italy
facility: AOUI
Study officials:
References: