Clinical trial NCT02624011: Influence of Sedentary Time on Skeletal Muscle Protein Metabolism
Official title: Influence of Increased Sedentary Time on Long-term Rates of Skeletal Muscle Protein Synthesis in Young Adults
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Sedentary Lifestyle
Lead sponsor: University of Birmingham
Sponsor class: OTHER
Collaborators: name: Maastricht University
First posted: 2015-12-08
Enrollment: 8
Sex: MALE
Minimum age (years): 18
Maximum age (years): 35
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: NA
Intervention model: SINGLE_GROUP
Primary purpose: BASIC_SCIENCE

Brief summary: This study will investigate the influence of increased sedentary time on long-term measures of muscle protein synthesis and metabolic health. The investigators will test the hypothesis that increased time spent in sedentary behaviours will lead to a reduction in long-term measures of muscle protein synthesis and compromised metabolic health.

Detailed description: Sarcopenia, the loss of muscle mass with age, is thought to be accelerated by an inactive, sedentary lifestyle. Increased sedentary time has consistently been associated with lower muscle mass and compromised metabolic health. However, there is currently a lack of direct evidence to support these associations.

Therefore, this study will investigate whether increased sedentary time (reduced step count and exercise cessation) directly influences long-term measures of muscle protein synthesis and metabolic health in young, active adults. Following a 7 day period of normal habitual physical activity, participants will undertake a 7 day period of step reduction and exercise cessation. It is hypothesised that 7 days of increased sedentary time will result in a reduction in long-term rates of muscle protein synthesis and a worsening of metabolic health.

Eligibility criteria: Inclusion Criteria:

* Body mass index (18.5-29.99 kg/m2)
* Active - defined as: ≥ 7000 steps per day (assessed during screening) and taking part in regular exercise (defined as participation in ≥ 3 sessions of aerobic and/or resistance exercise type sessions a week for ≥ 6 months prior to recruitment)
* Good general health

Exclusion Criteria:

* Lidocaine allergy
* Hypertension (≥140/90 mmHg)
* Current participation in another clinical study
* Previous participation in this study
* Bleeding disorder/s
* Current or recent smoker
* Past history of substance abuse and/or taking prescription or non-prescription medication (e.g., beta-blockers, insulin or thyroxine) or supplements that may influence normal metabolic responses.
* Participants who have previously (within 5 years of the present study) had 4 or more muscle biopsies obtained from the thigh quadriceps region will be ineligible.

Primary outcomes:
measure: Muscle protein synthesis (FSR %/day)
timeFrame: 0-14 days
Secondary outcomes:
measure: Rate of fat oxidation at rest (g/min)
timeFrame: For 20 minutes at day 7 and day 14
measure: Rate of fat oxidation during an oral glucose tolerance test (g/min)
timeFrame: At 0, 30, 60, 90 and 120min of the oral glucose tolerance test at day 7 and day 14
measure: Rate of carbohydrate oxidation at rest (g/min)
timeFrame: For 20 minutes at day 7 and day 14
measure: Rate of carbohydrate oxidation during an oral glucose tolerance test (g/min)
timeFrame: At 0, 30, 60, 90 and 120min of the oral glucose tolerance test at day 7 and day 14
measure: Blood glucose concentration
timeFrame: At 0, 30, 60, 90 and 120min of the oral glucose tolerance test at day 7 and day 14
measure: Blood insulin concentration
timeFrame: At 0, 30, 60, 90 and 120min of the oral glucose tolerance test at day 7 and day 14
measure: Blood fatty acid concentration
timeFrame: At 0, 30, 60, 90 and 120min of the oral glucose tolerance test at day 7 and day 14
Other outcomes:
Locations:
city: Birmingham
country: United Kingdom
facility: School of Sport Exercise and Rehabilitation Sciences, University of Birmingham
Study officials:
affiliation: University of Birmingham
name: Brandon J Shad, BSc
role: PRINCIPAL_INVESTIGATOR
References: