Clinical trial NCT06186050: Effects of a High-fat Meal on Exercise-mediated Sympatholysis
Official title: Effects of a High-fat Meal on Exercise-mediated Sympatholysis
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Diet, Healthy
Keywords: Functional sympatholysis
Exercise
Lead sponsor: University of Guelph
Sponsor class: OTHER
First posted: 2023-12-29
Enrollment: 15
Sex: ALL
Minimum age (years): 18
Maximum age (years): 30
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: CROSSOVER
Primary purpose: BASIC_SCIENCE

Brief summary: The goal of this trial is to examine the effects of a single high-fat meal with or without the co-ingestion of dietary nitrate on exercise-mediated sympatholysis. The main questions it aims to answer are:

1. What are the mechanisms responsible for reduced exercise-mediated sympatholysis following a high-fat meal
2. Can dietary nitrate prevent the declines in exercise-mediated sympatholysis

Participants will be asked to complete three study visits in a randomized order:

1. Low-fat meal
2. High-fat meal
3. High-fat meal plus dietary nitrate

Exercise-mediated sympatholysis will be measured using Doppler ultrasound via a protocol involving rhythmic handgrip exercise and a lower body negative pressure stimulus. Sympatholysis will be assessed before each meal, and at 1, 2, and 3 hours post-prandial.


Eligibility criteria: Inclusion Criteria:

* 18-30 years of age
* Healthy, free of known cardiovascular, metabolic, or musculoskeletal disease

Exclusion Criteria:

* Diagnosed cardiovascular or metabolic disease, injury preventing handgrip exercise, prescription of chronic medications other than oral contraceptives.
* Inability to abstain from drugs (except oral contraceptives), caffeine, alcohol, strenuous physical activity, mouthwash, and multivitamins prior to study

Primary outcomes:
measure: Forearm vascular conductance
timeFrame: Before intervention and 1, 2, 3 hours post-intervention
measure: Forearm blood flow
timeFrame: Before intervention and 1, 2, 3 hours post-intervention
measure: Systolic and Diastolic Blood Pressure
timeFrame: Before intervention and 1, 2, 3 hours post-intervention
Secondary outcomes:
measure: Plasma levels of Norepinephrine
timeFrame: Blood collected before intervention and 1, 2, 3 hours post-intervention. Blood frozen at -80 degrees Celsius and analyzed at later date
measure: Plasma levels of Nitrate/Nitrite concentration
timeFrame: Blood collected before intervention and 1, 2, 3 hours post-intervention. Blood frozen at -80 degrees Celsius and analyzed at later date
measure: Blood Glucose concentrations
timeFrame: Blood collected before intervention and 1, 2, 3 hours post-intervention. Blood analyzed immediately after collection at each time point
measure: Plasma levels of Triglycerides
timeFrame: Blood collected before intervention and 1, 2, 3 hours post-intervention. Blood frozen at -80 degrees Celsius and analyzed at later date
Other outcomes:
Locations:
city: Guelph
country: Canada
facility: University of Guelph - Human Cardiovascular Physiology Laboratory
state: Ontario
Study officials:
affiliation: University of Guelph
name: Philip Millar, PhD
role: PRINCIPAL_INVESTIGATOR
References: