Clinical trial NCT02557165: Impact of COPD on Muscle Regeneration
Official title: Impact of Chronic Obstructive Pulmonary Disease on Peripheral and Respiratory Muscle Regeneration
Study type: OBSERVATIONAL
Overall status: UNKNOWN
Conditions: COPD
Keywords: Muscle regeneration
Lead sponsor: Laval University
Sponsor class: OTHER
Collaborators: name: GlaxoSmithKline
First posted: 2015-09-23
Enrollment: 27
Sex: ALL
Minimum age (years): 40
Maximum age (years): 80
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: no
Observational model: CASE_CROSSOVER
Time perspective: PROSPECTIVE
Sampling method: NON_PROBABILITY_SAMPLE
Study population: Patient with a scheduled thoracic surgery

Brief summary: Muscle weakness and atrophy are important consequences of chronic obstructive pulmonary disease (COPD) and can affect both respiratory and peripheral muscles. Impaired muscle regeneration is thought to be implicated in the process of muscle atrophy. In this study the investigators will evaluate muscle regenerative potential of the Vastus Lateralis (VL) and diaphragm of patients with COPD, compared to patients with normal lung function.

Detailed description: Preliminary results suggest that the activation and differentiation processes of satellite cells isolated from the VL of patients with COPD could be altered. The investigators propose to compare the regenerative potential of the VL and the diaphragm in 15 patients with COPD and 15 individuals with normal lung function undergoing a thoracic surgery. From muscle biopsies, satellite cells will be isolated and cultured to assess their proliferation and differentiation rates, and the expression level of myogenic effectors (MyoD, Myf5, myogenin, and MRF4). Alterations in these measurements will confirm the hypotheses that the regenerative potential is lower in both muscle groups in patients with COPD compared to controls and that in COPD, the regenerative potential is lower in VL compared to the diaphragm.

Eligibility criteria: Inclusion Criteria:

* Patient with a scheduled thoracic surgery
* ≥ 40 years
* Smoking cessation ≥ 1 month
* Smoking history ≥ 10 pack-year

Exclusion Criteria:

* Subjects who received either chemiotherapy or radiotherapy
* Hypoxemia
* Body weight loss ≥ 10%
* Use of systemic corticosteroid in the last month

Primary outcomes:
measure: Muscle satellite cell proliferation index
timeFrame: Sequential analyses performed over a period of 2 weeks after tissue collection.
measure: Muscle satellite cell differentiation index
timeFrame: Sequential analyses performed over a period of 2 months after tissue collection.
Secondary outcomes:
Other outcomes:
Locations:
Study officials:
affiliation: Laval University
name: Richard Debigare, PhD, PT
role: PRINCIPAL_INVESTIGATOR
References:
citation: Theriault ME, Pare ME, Lemire BB, Maltais F, Debigare R. Regenerative defect in vastus lateralis muscle of patients with chronic obstructive pulmonary disease. Respir Res. 2014 Mar 25;15(1):35. doi: 10.1186/1465-9921-15-35.
citation: Theriault ME, Pare ME, Maltais F, Debigare R. Satellite cells senescence in limb muscle of severe patients with COPD. PLoS One. 2012;7(6):e39124. doi: 10.1371/journal.pone.0039124. Epub 2012 Jun 13.
citation: Caron MA, Debigare R, Dekhuijzen PN, Maltais F. Comparative assessment of the quadriceps and the diaphragm in patients with COPD. J Appl Physiol (1985). 2009 Sep;107(3):952-61. doi: 10.1152/japplphysiol.00194.2009. Epub 2009 Apr 9.