Clinical trial NCT03080129: Microbiome and Sarcopenia in Patients With Liver Cirrhosis
Official title: Microbiome and Sarcopenia in Patients With Liver Cirrhosis: A Prospective Controlled Cohort Study
Study type: INTERVENTIONAL
Overall status: RECRUITING
Phase: NA
Conditions: Sarcopenia
Liver Cirrhosis
Keywords: Liver cirrhosis
Sarcopenia
Microbiome
Lead sponsor: Medical University of Graz
Sponsor class: OTHER
First posted: 2017-03-15
Enrollment: 120
Sex: ALL
Minimum age (years): 18
Maximum age (years): 100
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: yes
Allocation: NON_RANDOMIZED
Intervention model: PARALLEL
Primary purpose: BASIC_SCIENCE

Brief summary: Protein-energy malnutrition (PEM) occurs in 65-90% of patients with liver cirrhosis. Severity of malnutrition correlates with progression of liver disease and leads to sarcopenia in 30-70% of cirrhotic patients. Malnutrition and sarcopenia are associated with an increased risk of complications and mortality.

In cirrhosis the gut microbiome is altered leading to increased gut permeability, bacterial translocation and inflammation. Since the microbiome is involved in nutrient uptake and metabolism, it is hypothesized that microbiome alterations contribute to sarcopenia. A prospective controlled cohort study to investigate the interrelation of microbiome changes and sarcopenia in cirrhosis will be conducted. Furthermore the effect of nutritional interventions on the microbiome in cirrhosis will be studied. From this study information on how the gut microbiome composition and sarcopenia are associated in cirrhosis and if modulation of the gut microbiome by nutritional interventions is feasible will be collected.

Detailed description: Scientific background

Protein-energy malnutrition (PEM) occurs in 65-90% of patients with chronic liver disease. PEM is caused by various factors including poor dietary intake, loss of appetite, decreased hepatic protein synthesis, malabsorption and hypermetabolism. It is associated with an increased risk of complications including ascites, hepatic encephalopathy, variceal bleeding, hepatorenal syndrome and mortality. There is a direct relation between the progression of the liver disease and the severity of malnutrition.

Malnutrition and sarcopenia in liver cirrhosis patients

PEM leads to sarcopenia as a common, but frequently overlooked, complication. Sarcopenia is defined as a decrease in muscle mass two standard deviations below the healthy young adult mean. Sarcopenia is associated with aging, chronic diseases and malignancy. To determine the severity of muscle wasting, computed tomography scan (CT) or magnetic resonance imaging (MRI) are an objective and reproducible technique. Sarcopenia negatively impacts on survival, correlates with the risk of infections, increases surgical risk and leads to a poor quality of life. Besides PEM also inflammation is of importance in the development of sarcopenia.

Diversity in the microbiome in patients with liver cirrhosis and association with sarcopenia.

The gut microbiome of liver cirrhosis patients is altered compared to healthy individuals. Dysbiosis leads to an increased gut permeability, bacterial translocation and inflammation. This contributes to fibrogenesis and may also be related to hepatocarcinogenesis. Hence, new treatment approaches in cirrhosis focus on changing the microbial landscape.

Modulation the gut microbiome may also be a strategy to reverse sarcopenia by reducing systematic inflammation.

Hypothesis and aims

There is an association between gut microbiome composition, gut permeability and the existence of sarcopenia in cirrhotic patients.

Primary hypothesis: Diversity of the gut microbiome is reduced in liver cirrhosis patients with sarcopenia compared to those without sarcopenia or healthy controls.

Secondary hypotheses: There is an association between gut microbiome composition, biomarker of gut permeability and bacterial translocation with the presence of sarcopenia in cirrhosis. Oral nutrition supplements (ONS) can influence the composition of the gut microbiome, gut permeability, bacterial translocation and inflammation. Sarcopenia can be diagnosed from patients portraits.

Aims: to investigate:

* the composition of the gut microbiome
* biomarkers of gut permeability, bacterial translocation and inflammation
* the incidence and severity of sarcopenia
* the impact of oral nutrition supplements (ONS) on the gut microbiome
* whether artificial intelligence can be used to diagnose sarcopenia from face portraits.

Eligibility criteria: Inclusion Criteria:

* Hospitalized patients for any reason with clinical/radiological/histological diagnosis of cirrhosis
* Age \>18y
* Informed consent
* CT/MRI scan within +/-14 days of the baseline study visit

Exclusion Criteria:

* Hepatic encephalopathy \> grade 2 and or other cognitive disorder not allowing informed consent
* advanced hepatocellular carcinoma
* Any other condition or circumstance, which, in the opinion of the investigator, would affect the patient's ability to participate in the protocol

Primary outcomes:
measure: Alpha diversity
timeFrame: day 1
Secondary outcomes:
measure: Zonulin
timeFrame: day 1
measure: diamino-oxidase
timeFrame: day 1, day 7
measure: Calprotectin
timeFrame: day 1
measure: Gut permeability
timeFrame: change between day 1 and day 7
measure: taxonomic composition of the microbiome
timeFrame: day 1
measure: taxonomic composition of the microbiome
timeFrame: change between day 1 and day 7
measure: lipopolysaccharide
timeFrame: day 1
measure: sCD14
timeFrame: day 1
measure: lipopolysaccharide binding protein
timeFrame: day 1
measure: bacterial DNA
timeFrame: day 1
measure: bacterial translocation
timeFrame: change between day 1 and day 7
measure: cytokine panel
timeFrame: day 1
measure: carboxylated proteins
timeFrame: day 1
measure: advanced oxidation end products
timeFrame: day 1
measure: inflammation
timeFrame: change between day 1 and day 7
measure: myostatin
timeFrame: day 1
measure: fibroblast growth factor 21
timeFrame: day 1
measure: insulin like growth factor 1
timeFrame: day 1
measure: Irisin
timeFrame: day 1
measure: nutritional status
timeFrame: day 1
measure: Sarcopenia
timeFrame: day 1
measure: face portrait
timeFrame: day 1
Other outcomes:
Locations:
city: Graz
country: Austria
facility: Medical University Graz
Study officials:
References: