Clinical trial NCT05732207: Cerebellar Involvement in Alcohol Use Disorder (AUD)
Official title: Investigation of Cerebellar Involvement in AUD
Study type: INTERVENTIONAL
Overall status: RECRUITING
Phase: NA
Conditions: Alcohol Use Disorder
Keywords: AUD
heavy drinking
hazardous drinking
Lead sponsor: Johns Hopkins University
Sponsor class: OTHER
Collaborators: name: National Institute on Alcohol Abuse and Alcoholism (NIAAA)
First posted: 2023-02-16
Enrollment: 122
Sex: ALL
Minimum age (years): 25
Maximum age (years): 55
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: NON_RANDOMIZED
Intervention model: PARALLEL
Intervention model description: AUD and age- and gender matched control participants will be tested under sham, cathodal, and anodal tDCS (in counterbalanced order)
Primary purpose: BASIC_SCIENCE

Brief summary: The goal of this observational and interventional study is to better understand the involvement of the cerebellum in the brain reward system in persons with alcohol use disorder (AUD). The main questions it aims to answer are:

1. What is the nature of cerebellar input to the ventral tegmental area (VTA) in the brain reward system, and how is it perturbed in AUD?
2. What is the relationship between measures of cerebellar integrity and magnitude of reward activation to alcohol-related cues in cerebellar, VTA and other brain reward structures?
3. What is the therapeutic potential of cerebellar transcranial direct current stimulation (tDCS) for modulating alcohol cue reactivity, associated alcohol craving, and cerebellar - VTA functional connectivity in the brain reward system? Persons with AUD will be compared with healthy control participants.

Detailed description: Recent animal studies have provided new evidence that the cerebellum may have a stronger link to the reward system of the brain than was previously recognized. Direct projections from cerebellar deep nuclei (DN) to the ventral tegmental area (VTA) have been identified, and stimulation of these cerebellar afferents to the VTA was found to be rewarding. Such findings raise the possibility that cerebellar dysfunction could contribute substantially to addiction via a cerebellar influence over VTA. Consistent with animal findings, the investigators have found in human functional MRI (fMRI) preliminary data strong cerebellar and VTA activation in response to alcohol cues relative to non-alcohol stimuli in patients with alcohol use disorder (AUD) compared to controls, and close coupling observed between DN and VTA activation. Studying AUD and control participants, this project will address three important questions. The first is: What is the nature of cerebellar input to the VTA, and how is it perturbed in AUD? A number of investigations have suggested that when a stimulus is presented, the cerebellum generates a prediction of events that will follow based on prior associative learning, and then compares predicted and actual outcomes to generate a prediction error. The investigators hypothesize that these functions are disrupted in AUD. The investigators' preliminary data show that when an expected stimulus does not occur, a strong prediction error signal in the form of increased functional connectivity (FC) between cerebellum and its projection target is observed, and the investigators found an analogous increase in DN-VTA FC, that was abnormal in AUD patients, when alcohol pictures were presented. In Aim 1, using fMRI and a monetary incentive task, the investigators will investigate if DN-VTA FC reflects reward prediction and/or positive or negative reward prediction error. The second question is: Is the amount of activation in brain reward centers that is elicited by alcohol stimuli related to the amount of dysfunction in the cerebellum? In Aim 2 the investigators will investigate 2 measures of cerebellar integrity to determine the relationship with the magnitude of alcohol cue related activation in cerebellar, VTA, and other reward structures, and with DN-VTA FC: (1) The timing of the undershoot of the cerebellar hemodynamic response function (HRF), which has been found to be correlated with number of lifetime drinks; and (2) classical eyeblink conditioning, for which the cerebellum is necessary. The third question is: Can abnormal cerebellar activation and FC, as well as alcohol craving, be reduced by non-invasive cerebellar stimulation? In Aim 3, Using fMRI combined with cerebellar transcranial direct current stimulation (tDCS) during a cue reactivity task, the investigators hypothesize that in AUD participants cerebellar and VTA activation will be reduced, DN-VTA FC will be normalized, and alcohol craving will be reduced. The investigators will examine, using both resting state fMRI and psychophysiological interaction analysis, the effects of tDCS on FC among important structures of the reward system as well as on DN-VTA FC. These investigations will lead to a better understanding of the involvement of the cerebellum in AUD, as well as the therapeutic potential of cerebellar modulation.

Eligibility criteria: Inclusion Criteria:

* completed at least 8 years of education

Exclusion Criteria:

* Estimated Intelligence Quotient (IQ) \< 90
* less than 5th grade reading level
* Left handed
* Non-fluent in English
* current drug use disorder other than alcohol (except nicotine and caffeine) and or recent drug use in the last 90 days
* Positive breath alcohol level at time of MRI scan or discrepancies between alcohol biomarker and self-report that cannot be resolved
* Exhibiting symptoms of alcohol withdrawal on visit 1 assessment
* Significant current psychiatric distress and or treatment
* History of any central nervous system disorder, presence of a seizure disorder, or use of anticonvulsant medication in the past 3 months
* any serious medical condition detected on assessment or by medical record review; or have liver function tests more than three times normal at screening
* History of metal implantation that would preclude MRI scanning; or other implants, pumps, pacemakers that would be contraindications for MRI scanning
* Abnormal MRI scan or history of significant closed head trauma
* Evidence of dementia
* For women, pregnancy

Primary outcomes:
measure: Craving for alcohol during the cue reactivity task as assessed by a rating scale
timeFrame: 28 minutes
measure: Resting state functional connectivity during tDCS
timeFrame: 28 minutes
measure: Brain activation to alcohol cues
timeFrame: 28 minutes
measure: Brain functional connectivity to alcohol vs non-alcohol cues
timeFrame: 28 minutes
measure: Brain activation related to reward prediction during the monetary incentive task
timeFrame: 18 minutes
measure: Brain functional connectivity related to reward prediction during the monetary incentive task
timeFrame: 18 minutes
measure: Brain activation to reward prediction error during the monetary incentive task
timeFrame: 18 minutes
measure: brain functional connectivity to reward prediction error during the monetary incentive task
timeFrame: 18 minutes
measure: Percentage of trials with a conditioned response during the classical eyeblink conditional task
timeFrame: 21 minutes
measure: Mean time (in milliseconds) at which peak of conditioned response occurs during the classical eyeblink conditional task
timeFrame: 21 minutes
Secondary outcomes:
measure: Baseline cerebral blood flow (CBF) measured from a CBF MRI scan
timeFrame: 4 minutes and 40 seconds
measure: Behavioral accuracy during the monetary incentive task
timeFrame: 18 minutes
Other outcomes:
Locations:
city: Baltimore
country: United States
facility: Johns Hopkins University School of Medicine
state: Maryland
Study officials:
affiliation: Johns Hopkins University
name: John E Desmond, PhD
role: PRINCIPAL_INVESTIGATOR
References: