Clinical trial NCT06870786: Effect of Vitamin D Deficiency on the Structure of Titanium Platelet-Rich Fibrin
Official title: Effect of Vitamin D Deficiency on the Structure of Titanium Platelet-Rich Fibrin(T-Prf)
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: PRF
Vitamin D Deficiency
Vitamin Deficiency
Lead sponsor: Abant Izzet Baysal University
Sponsor class: OTHER
First posted: 2025-03-11
Enrollment: 24
Sex: ALL
Minimum age (years): 18
Maximum age (years): 65
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: PARALLEL
Primary purpose: OTHER

Brief summary: ABSTRACT The aim of the study was to compare the macroscopic properties, chemical degradation levels, and fibrin network structures of T-PRF membranes obtained from systemically healthy individuals with optimal vitamin D levels, with vitamin D-deficient individuals who reached optimal levels after three months of vitamin D supplementation.

A total of 48 participants, 24 with vitamin D deficiency and 24 with optimal vitamin D levels, were included in the study. The macroscopic properties (weight, width, length, density), chemical degradation levels and fibrin network structures of the obtained T-PRF membranes were evaluated using scanning electron microscopy (SEM) and light microscopy.

Detailed description: Blood samples taken from individuals with the help of a 20 ml syringe were immediately transferred to 2 10 ml titanium tubes and placed opposite each other to ensure balance in the tabletop centrifuge device in order to be properly separated in the centrifuge process. To obtain T-PRF, it was centrifuged in the IntraSpin™ device at 2700 rpm centrifugal force for 12 minutes.

After centrifugation, the fibrin in the middle of the 3 layers formed in the titanium tube was held and pulled with the help of a press. Then, the red layer in the lower layer, where erythrocytes were dense, was separated from the fibrin layer with the help of scissors and the membrane was obtained by pressing for 2 minutes using the own weight of the PRF box.

Eligibility criteria: Inclusion Criteria:

* Individuals aged 20-55
* Being systemically healthy
* Not using any medication regularly
* Not taking additional vitamin D from outside
* Patients who met the conditions of not having used antibiotics for the last three months were included in the study.

Exclusion Criteria:

* People who use cigarettes (tobacco) and alcohol
* Those with platelet function and clotting disorders
* Those who are pregnant or breastfeeding
* Those who use medications that affect the natural clotting process
* Those with a history of infection in the last three months
* Those receiving immunosuppressive therapy
* Those who do not consent to volunteer

Primary outcomes:
measure: microscopic analysis
timeFrame: From enrollment to the end of treatment at 3 months
Secondary outcomes:
measure: Scanning Electron Microscope (SEM) Examination
timeFrame: From enrollment to the end of treatment at 3 months
measure: T-PRF Degradation of Membranes
timeFrame: From enrollment to the end of treatment at 3 months
Other outcomes:
Locations:
city: Bolu
country: Turkey
facility: Department of Periodontology, Faculty of Dentistry, Bolu Abant İzzet Baysal University,
Study officials:
References: