Clinical trial NCT02456870: Expandability of Aging Adipose Tissue
Official title: Aging Subcutaneous Adipose Tissue Response to Overfeeding
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Aging
Obesity
Keywords: Adipose tissue
Overfeeding
Lead sponsor: University of Colorado, Denver
Sponsor class: OTHER
Collaborators: name: Colorado State University
First posted: 2015-05-29
Enrollment: 20
Sex: MALE
Minimum age (years): 25
Maximum age (years): 75
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: yes
Allocation: NON_RANDOMIZED
Intervention model: SINGLE_GROUP
Primary purpose: BASIC_SCIENCE

Brief summary: The purpose of this pilot study is to determine whether adipose tissue loses some of its ability to expand in response to overfeeding in older compared to younger men. The investigators hypothesize that the adipose tissue response to overfeeding may be weakened in older when compared to younger men, thereby leading to worsened ectopic storage depots for older men.

Detailed description: This pilot study will begin to address whether adipose tissue biology changes with advancing age. Specifically, we will test whether adipose tissue loses some of its ability to expand in response to overfeeding in older compared to younger men. To test this possibility the Investigators will recruit overweight and mildly obese older (age 55-75y) and younger (age 25-40y) men and collect adipose tissue biopsies before and after 7 days of overfeeding. The investigators will test how well the cells in adipose tissue expand in response to overfeeding and whether this differs between younger and older men.

Eligibility criteria: Inclusion Criteria:

* Men
* Aged 25-40y OR 55-75y
* BMI 28-35 kg/m2
* Weight stable (±2kg over past 3 mo)
* Sedentary to moderately active (\<3 d/wk of exercise lasting \>20min)
* Non-smokers (never used or quit \>7yrs prior)

Exclusion Criteria:

* Women
* Currently using hormones, glucose or lipid lowering medication
* Engaged in exercise or diet program
* Currently gaining or losing weight or using weight loss drugs
* Diabetes mellitus (diagnosis, treatment, FBG ≥126 mg/dL)
* Uncontrolled metabolic disorders (e.g., thyroid)

Primary outcomes:
measure: Change in adipose-derived stem cell (ASC) differentiation (Delta%oil red O+)
timeFrame: 1 wk of overfeeding
Secondary outcomes:
measure: Change in ASC proliferation (Delta rate of cell growth)
timeFrame: 1 wk of overfeeding
measure: Change in expression of C/EBPalpha, C/EBPbeta, PPARgamma2, SREBP1c, SREBf1a, and Wnt10b (fold-changes in mRNA expression)
timeFrame: 1 wk overfeeding
measure: Change in intramyocellular (IMCL) di-saturated diacylglycerol (DAG) localization (Delta %membrane:cytosol)
timeFrame: 1 week overfeeding
Other outcomes:
Locations:
city: Aurora
country: United States
facility: Univeristy of Colorado, Anschutz Medical Campus
state: Colorado
Study officials:
affiliation: University of Colorado, Denver
name: Rachael E Van Pelt, PhD
role: PRINCIPAL_INVESTIGATOR
References:
citation: Kim JY, van de Wall E, Laplante M, Azzara A, Trujillo ME, Hofmann SM, Schraw T, Durand JL, Li H, Li G, Jelicks LA, Mehler MF, Hui DY, Deshaies Y, Shulman GI, Schwartz GJ, Scherer PE. Obesity-associated improvements in metabolic profile through expansion of adipose tissue. J Clin Invest. 2007 Sep;117(9):2621-37. doi: 10.1172/JCI31021.
citation: Caso G, McNurlan MA, Mileva I, Zemlyak A, Mynarcik DC, Gelato MC. Peripheral fat loss and decline in adipogenesis in older humans. Metabolism. 2013 Mar;62(3):337-40. doi: 10.1016/j.metabol.2012.08.007. Epub 2012 Sep 19.
citation: Tchoukalova YD, Votruba SB, Tchkonia T, Giorgadze N, Kirkland JL, Jensen MD. Regional differences in cellular mechanisms of adipose tissue gain with overfeeding. Proc Natl Acad Sci U S A. 2010 Oct 19;107(42):18226-31. doi: 10.1073/pnas.1005259107. Epub 2010 Oct 4.