Clinical trial NCT02926196: Adjuvant Treatment for High-risk Triple Negative Breast Cancer Patients With the Anti-PD-l1 Antibody Avelumab
Official title: Adjuvant Treatment for High-risk Triple Negative Breast Cancer Patients With the Anti-PD-l1 Antibody Avelumab: A Phase III Randomized Trial. Sponsor: Dipartimento di Scienze Chirurgiche, Oncologiche e Gastroenterologiche, Università di Padova
Study type: INTERVENTIONAL
Overall status: ACTIVE_NOT_RECRUITING
Phase: PHASE3
Conditions: Triple Negative Breast Neoplasms
Keywords: ER-Negative
PR-Negative
HER2-Negative
Lead sponsor: Istituto Oncologico Veneto IRCCS
Sponsor class: OTHER
Collaborators: name: University of Padova
name: Dipartimento di scienze chirurgiche, Oncologiche e Gastroenterologiche
First posted: 2016-10-06
Enrollment: 474
Sex: ALL
Minimum age (years): 18
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: no
Allocation: RANDOMIZED
Intervention model: PARALLEL
Primary purpose: TREATMENT

Brief summary: Phase III randomized trial of the anti-PD-L1 antibody avelumab as adjuvant or post-neoadjuvant treatment for high-risk triple negative breast cancer patients. The overall protocol-defined patient population will include the following two strata of patients:

* Stratum A - Patients who have completed treatment with curative intent including surgery of the primary tumor followed by adjuvant chemotherapy .
* Stratum B - Patients who have completed treatment with curative intent including neoadjuvant chemotherapy followed by surgery of the primary tumor and (if indicated) further adjuvant chemotherapy.

Detailed description: * to determine whether 1 year of adjuvant Avelumab improves disease-free survival (DFS) compared to observation in patients with high-risk primary triple negative breast cancer who have completed treatment with curative intent including surgery of the primary tumor and neo- or adjuvant chemotherapy (Stratum A \[surgery of the primary tumor followed by adjuvant chemotherapy\] and Stratum B \[neoadjuvant chemotherapy followed by surgery\] combined).
* to determine whether 1 year of adjuvant Avelumab improves disease-free survival (DFS) compared to observation in patients with high-risk primary triple negative breast cancer who have completed treatment with curative intent including neoadjuvant chemotherapy followed by surgery (Stratum B).
* to determine whether Avelumab improves overall survival (OS) compared to observation in patients with high-risk primary triple negative breast cancer who have completed treatment with curative intent including surgery of the primary tumor and neo- or adjuvant chemotherapy.
* to determine whether 1 year of adjuvant Avelumab improves disease-free survival (DFS) compared to observation in PD-L1-positive (as determined by a companion diagnostic test under development) patients with high-risk primary triple negative breast cancer who have completed treatment with curative intent including surgery of the primary tumor and neo- or adjuvant chemotherapy.

Eligibility criteria: Inclusion Criteria Stratum A (Adjuvant patients) \& B (Post-neoadjuvant patients)

1. Male or female subjects aged \> 18 years
2. Signed written informed consent before any trial-related procedure is undertaken that is not part of the standard patient management
3. Eastern Cooperative Oncology Group (ECOG) performance status 0 or 1
4. Patients must have completed treatment with curative intent including: surgery and adjuvant chemotherapy.
5. Patients must have completed adjuvant chemotherapy including at least 3 courses of an anthracycline agent and 3 courses of a taxane agent. Patients who received dose-dense regimens and those who received carboplatin as part of the adjuvant treatment are eligible.
6. No more than 10 weeks may elapse between the completion of last adjuvant treatment (adjuvant chemotherapy or surgery) and randomization.

8. Normal organ and marrow function

1. White blood count (WBC) greater than or equal to 2.5 x109/L
2. Absolute neutrophil count (ANC) greater than or equal to 1.5 x109/L
3. Absolute lymphocyte count greater or equal to 0.5 x109/L
4. Platelet count greater than or equal to 100 x109/L
5. Hemoglobin greater than or equal to 9 g/dL
6. Serum creatinine less or equal to 1.5 x the upper limit of laboratory normal range (ULN)
7. Adequate hepatic function defined by a total bilirubin level less or equal to 1.5 x ULN range and AST and ALT levels less or equal than 2.5 x ULN for all subjects. For patients with known Gilbert's syndrome, total bilirubin levels less or equal than 2 x ULN range (with direct bilirubin less than ULN) will be accepted.

   9. Highly effective contraception (i.e. methods with a failure rate of less than 1 % per year) for both male and female subjects if the risk of conception exists (Note: The effects of the trial treatment on the developing human fetus are unknown; thus, women of childbearing potential and men must agree to use highly effective contraception, defined in Appendix A or as stipulated in national or local guidelines. Highly effective contraception must be used 28 days prior to first trial treatment administration, for the duration of trial treatment, and at least for 60 days after stopping trial treatment. Should a woman become pregnant or suspect she is pregnant while she or her partner is participating in this trial, the treating physician should be informed immediately).

   10. Ability to understand and willingness to sign a written informed consent.

Inclusion Criteria Stratum A (Adjuvant patients)

1. Non-metastatic, histologically confirmed primary invasive breast carcinoma
2. Triple negative breast cancer: hormone receptor negative (ER \< 10% and PgR \< 10%) and HER2 negative (IHC 0/1+ or ISH non-amplified), as defined by the local pathology laboratory. In case of discordance between pre-operative core-biopsy and the surgical sample, the receptor assessment performed on the surgical sample has to be considered for inclusion criteria evaluation.
3. Availability of a formalin-fixed, paraffin-embedded block containing tumor tissue or at least 7 unstained tumor slides.
4. Adequately excised: patients must have undergone either breast-conserving surgery or mastectomy/nipple- or skin-sparing mastectomy. The margins of the resected specimen should be free of invasive tumor and ductal carcinoma in situ (no ink on tumor). In the case of breast-conserving surgery patients with margins positive for lobular carcinoma in situ (LCIS) are eligible without additional resection. For patients who undergo mastectomy, patients with a microscopic positive deep margin are eligible, provided they will receive radiotherapy on chest wall.
5. Patients must have had axillary lymph node dissection for evaluation of pathologic nodal status. Only patients in one of the following stage categories will be eligible:

   * if 4 or more metastatic lymph nodes, any pT
   * if 1 to 3 metastatic lymph nodes, pT \>2 cm
   * if no metastatic lymph nodes, pT \>5 cm

Inclusion criteria:

Stratum B (Post-neoadjuvant patients)

1. Non-metastatic histologically confirmed invasive breast carcinoma.
2. Triple negative breast cancer: hormone receptor negative (ER \< 10% and PgR \< 10%) and HER2 negative (IHC 0/1+ or ISH non-amplified), as defined by the local pathology laboratory. In case of discordance between the pre-treatment diagnostic core-biopsy and the surgical sample, the receptor assessment performed on the surgical sample has to be considered for inclusion criteria evaluation.
3. Adequately excised: patients should have undergone adequate tumor excision after preoperative chemotherapy, which means surgical removal of all clinically evident disease in the breast and lymph nodes.

   1. Breast surgery: patients must have undergone either breast-conserving surgery or mastectomy/nipple- or skin-sparing mastectomy. The margins of the resected specimen should be free of invasive tumor and ductal carcinoma in situ (no ink on tumor). In the case of breast-conserving surgery patients with margins positive for lobular carcinoma in situ (LCIS) are eligible without additional resection. For patients who undergo mastectomy, patients with a microscopic positive deep margin are eligible, provided they will receive radiotherapy on chest wall.
   2. Lymph node surgery:

   i. Axillary dissection without sentinel node evaluation is permitted after preoperative therapy.

   ii. In case of positive results from a fine-needle aspiration, core biopsy, or sentinel node biopsy performed prior to preoperative therapy, additional surgical evaluation of the axilla following preoperative therapy is required.

   iii. If sentinel node biopsy performed before preoperative therapy was negative, no additional surgical evaluation of the axilla is required after preoperative therapy.

   iv. Sentinel node after preoperative therapy is allowed if no evidence of axillary node involvement was documented by ultrasonography at diagnosis. If sentinel node biopsy after preoperative therapy is negative, no further additional surgical evaluation of the axilla is required. If sentinel node biopsy performed after preoperative therapy is positive, additional surgical evaluation of the axilla is recommended.
4. Pathologic evidence of residual invasive carcinoma in the breast and/or axillary lymph nodes on the surgical specimen obtained after preoperative therapy (ypT1micN0, ypT1micN0i+, ypT0N0i+ will be excluded).
5. Clinical stage at presentation: T1-4, N0-3, M0 (Exception: Patients with T1a/bN0 tumors at presentation will not be eligible).
6. No more than 10 weeks may elapse between the date of last treatment (surgery or post-surgery chemotherapy if indicated) and the date of randomization. In case of positive margins after the first intervention requiring additional resection.
7. Availability of a formalin-fixed, paraffin-embedded block containing tumor tissue or at least 7 unstained tumor slides (tumor sample from the diagnostic core-biopsy obtained before neoadjuvant chemotherapy). In case only 7 unstained slides from the bioptic sample will be available, the investigator must ensure that the sample contains tumor tissue by performing an hematoxylin and eosin staining.

Exclusion criteria: Stratum A (Adjuvant patients) \& B (Post-neoadjuvant patients)

1. Stage IV breast cancer.
2. History of any prior (ipsi- and/or contralateral) invasive breast carcinoma diagnosed within 10 years.
3. Synchronous bilateral breast cancer, unless both tumors confirmed as triple negative disease.
4. History of non-breast malignancies within the 5 years prior to study entry, except for the following: Carcinoma in situ (CIS) of the cervix, CIS of the colon, Basal cell and squamous cell carcinomas of the skin.
5. Prior organ transplantation, including allogeneic stem-cell transplantation.
6. Prior or concomitant treatment with any other investigational agents.
7. Prior therapy with any antibody / drug targeting T-cell coregulatory proteins (immune-checkpoints) such as PD-1, PD-L1, or cytotoxic T-lymphocyte antigen-4 (CTLA-4).
8. Concurrent anticancer treatment (for example, cytoreductive therapy, immune therapy, or cytokine therapy except for erythropoietin)
9. Major surgery for any reason, within 4 weeks of randomization and / or if the subject has not fully recovered from the surgery within 4 weeks of randomization.
10. Concomitant treatment with all herbal (alternative) remedies with immunostimulating properties (for example, mistletoe extract) or known to potentially interfere with major organ function (for example, hypericin).
11. Subjects receiving immunosuppressive agents (such as steroids) for any reason should be tapered off these drugs before initiation of the trial treatment (with the exception of subjects with adrenal insufficiency, who may continue corticosteroids at physiologic replacement dose, equivalent to ≤ 10 mg prednisone daily).
12. Significant acute or chronic infections including, among others:

    1. Known history of testing positive test for human immunodeficiency virus (HIV) or known acquired immunodeficiency syndrome.
    2. Hepatitis B virus (HBV) or hepatitis C virus (HCV) infection at screening (positive HBV surface antigen or HCV RNA if anti-HCV antibody screening test positive).
13. Active autoimmune disease that might deteriorate when receiving an immunostimulatory agent:

    1. Subjects with diabetes type I, vitiligo, psoriasis, hypo- or hyperthyroid disease not requiring immunosuppressive treatment are eligible.
    2. Subjects requiring hormone replacement with corticosteroids are eligible if the steroids are administered only for the purpose of hormonal replacement and at doses ≤ 10 mg or equivalent prednisone per day.
    3. Administration of steroids through a route known to result in a minimal systemic exposure (topical, intranasal, intro-ocular, or inhalation) are acceptable.
14. Administration of steroids through a route known to result in a minimal systemic exposure (topical, intranasal, intro-ocular, or inhalation) are acceptable.
15. Previous or ongoing administration of systemic steroids for the management of an acute allergic phenomenon is acceptable as long as it is anticipated that the administration of steroids will be completed in 14 days, or that the daily dose after 14 days will be ≤ 10 mg per day of equivalent prednisone.
16. Known severe hypersensitivity reactions to monoclonal antibodies (Grade ≥ 3 NCI-CTCAE v 4.03), any history of anaphylaxis, or uncontrolled asthma (that is, 3 or more features of partially controlled asthma).
17. Clinically significant (that is, active) cardiovascular disease: cerebral vascular accident /stroke (\< 6 months prior to enrollment), myocardial infarction (\< 6 months prior to enrollment), unstable angina, congestive heart failure (New York Heart Association Classification Class ≥ II), or serious uncontrolled cardiac arrhythmia requiring medication.
18. All other significant diseases (for example, inflammatory bowel disease), which, in the opinion of the Investigator, might impair the subject's tolerance of trial treatment.
19. Any psychiatric condition that would prohibit the understanding or rendering of informed consent.
20. Vaccination within 4 weeks of the first dose of avelumab and while on trial is prohibited except for administration of inactivated vaccines (for example, inactivated influenza vaccines).
21. Known alcohol or drug abuse.
22. Persisting toxicity related to prior therapy of Grade \> 1 NCI-CTCAE v 4.03 (except for grade 2 radiodermatitis and grade 2 neuropathy).
23. Current pregnancy and/or lactation. Refusal to adopt adequate contraception methods.

Stratum B (Postneoadjuvant patients)

1. No invasive residual disease in the breast and axilla at pathological examination after neoadjuvant chemotherapy. ypT1micN0, ypT1micN0i+, ypT0N0i+ will also be excluded.

Primary outcomes:
measure: Disease free survival
timeFrame: Up to 5 years after randomization
measure: Disease free survival in PD-L1-positive patients
timeFrame: Up to 5 years after randomization
Secondary outcomes:
measure: Overall survival
timeFrame: Up to 5 years after randomization
measure: Safety profile
timeFrame: From Baseline up to 5 years after randomization
Other outcomes:
Locations:
city: Bergamo
country: Italy
facility: Ospedale di Bergamo
state: BG
city: Bologna
country: Italy
facility: Policlinico Sant'Orsola Malpighi
state: BO
city: Bologna
country: Italy
facility: Ospedale di Bellaria
state: BO
city: Brindisi
country: Italy
facility: Azienda Sanitaria Locale Brindisi
state: BR
city: Brescia
country: Italy
facility: Azienda Spedali Civili di Brescia
state: BS
city: Cuneo
country: Italy
facility: A.S.O. S.Croce e Carle di Cuneo
state: CN
city: Catania
country: Italy
facility: AOU Policlinico "Vittorio. Emanuele
state: CT
city: Catania
country: Italy
facility: ARNAS Garibaldi,
state: CT
city: Cona
country: Italy
facility: Arcispedale S. Anna
state: FE
city: Genova
country: Italy
facility: AOU San Martino IST Istituto Nazionale per la Ricerca sul Cancro IRCCS
state: GE
city: Grosseto
country: Italy
facility: Ospedale Misericordia di Grosseto
state: GR
city: Lucca
country: Italy
facility: ASL Lucca
state: LU
city: Milano
country: Italy
facility: Istituto Nazionale dei Tumori IRCCS
state: MI
city: Carpi
country: Italy
facility: Ospedale Ramazzini
state: MO
city: Modena
country: Italy
facility: Azienda Ospedaliero-Universitaria di Modena - Policlinico
state: MO
city: Palermo
country: Italy
facility: AOU Policlinico di Palermo
state: PA
city: Camposampiero
country: Italy
facility: Ospedale di Camposampiero
state: PD
city: Padova
country: Italy
facility: Istituto Oncologico Veneto IRCCS
state: PD
city: Aviano
country: Italy
facility: Centro di Riferimento Oncologico di Aviano (CRO)
state: PN
city: Prato
country: Italy
facility: AUSL 4
state: PO
city: Parma
country: Italy
facility: Azienda Ospedaliera Universitaria di Parma
state: PR
city: Rionero in Vulture
country: Italy
facility: CROB-IRCCS di Rionero in Vulture
state: PZ
city: Reggio Emilia
country: Italy
facility: IRCCS - Azienda Ospedaliera S.M. Nuova
state: RE
city: Trento
country: Italy
facility: Ospedale Civile Santa Chiara
state: TN
city: Candiolo
country: Italy
facility: I.R.C.C.S. - Fondazione del Piemonte per l'Oncologia
state: TO
city: Castelfranco Veneto
country: Italy
facility: Ospedale di Castelfranco Veneto
state: TV
city: Treviso
country: Italy
facility: Azienda ULSS 9 - Ca Foncello
state: TV
city: Udine
country: Italy
facility: A. O. U. Santa Maria della Misericordia
state: UD
city: Mirano
country: Italy
facility: Ospedale di Mirano
state: VE
city: Negrar
country: Italy
facility: Ospedale Sacro Cuore - Don Calabria
state: VR
city: Verona
country: Italy
facility: Policlinico G.B. Rossi
state: VR
city: Ancona
country: Italy
facility: Clinica Oncologica-Ospedali Riuniti Ancona
city: Asti
country: Italy
facility: Azienda Sanitaria Locale Di Asti
city: Belluno
country: Italy
facility: Ospedale Dell'Ulss N. 1 Belluno- Ospedale S. Martino Belluno
city: Bolzano
country: Italy
facility: Ospedale Centrale Di Bolzano
city: Chieti
country: Italy
facility: P.O. Clinicizz. 'Ss. Annunziata' Chieti
city: Como
country: Italy
facility: Asst Lariana
city: Cremona
country: Italy
facility: A.O. Istituti Ospedalieri - Cremona
city: Faenza-Ravenna-Lugo
country: Italy
facility: Azienda Unità Sanitaria Locale della Romagna
city: L'Aquila
country: Italy
facility: Ospedale San Salvatore
city: Lecce
country: Italy
facility: Ospedale Lecce - 'V Fazzi' (San Cesario)- Opedale Lecce - 'V.Fazzi'
city: Livorno
country: Italy
facility: Ospedale di Livorno
city: Macerata
country: Italy
facility: UOC Oncologia ASUR AV3 Macerata
city: Meldola
country: Italy
facility: I.R.S.T. Srl Irccs
city: Messina
country: Italy
facility: AOR Papardo
city: Mestre
country: Italy
facility: Ospedale dell'Angelo
city: Napoli
country: Italy
facility: Azienda Ospedaliera Universitaria Federico Ii
city: Napoli
country: Italy
facility: Istituto Nazionale Tumori - Fondazione Pascale,
city: Novara
country: Italy
facility: AOU Maggiore della Carità - SC Oncologia Novara
city: Pesaro Fano
country: Italy
facility: .O. Ospedali Riuniti Marche Nord- Ospedale San Salvatore - Pesaro
city: Piacenza
country: Italy
facility: Ospedale "Guglielmo Da Saliceto" Piacenza
city: Pisa
country: Italy
facility: Azienda Ospedaliero-Universitaria Pisana
city: Potenza
country: Italy
facility: Azienda Ospedaliera Regionale 'S. Carlo'- Ospedale San Carlo Di Potenza
city: Rimini
country: Italy
facility: Presidio Ospedaliero Rimini-Santarcangel- Ospedale "Infermi" Rimini
city: Roma
country: Italy
facility: Azienda Ospedaliera Complesso Ospedaliero San Giovanni - Addolorata
city: Roma
country: Italy
facility: Ifo - Istituto Nazionale Tumori Regina Elena (Ire)
city: Roma
country: Italy
facility: Ospedale Fatebenefratelli
city: Roma
country: Italy
facility: Policlinico Universitario Campus Biomedico
city: Roma
country: Italy
facility: U.O.C. di Oncologia Medica Interpresidio PO S.Pertini-S Eugenio-CTO Roma
city: Roma
country: Italy
facility: UOC Oncologia Osp. S.Andrea Un. La Sapienza Roma
city: Torino
country: Italy
facility: Ao Citta' Della Salute E Della Scienza D- Osp.S. Giov.Battista Molinette
city: Varese
country: Italy
facility: Ospedale Di Circolo E Fondazione Macchi - Varese
city: Bath
country: United Kingdom
facility: Royal United Hospitals Bath NHS Foundation Trust
city: Blackpool
country: United Kingdom
facility: Blackpool Teaching Hospital
city: Inverness
country: United Kingdom
facility: Raigmore Hospital
city: London
country: United Kingdom
facility: Royal Free Hospital
city: London
country: United Kingdom
facility: St Bartholomew's Hospital
city: Northwood
country: United Kingdom
facility: Hillingdon Hospitals NHS Foundation Trust and Mount Vernon Cancer Centre
city: Nottingham
country: United Kingdom
facility: Nottingham City Hospital
city: Southampton
country: United Kingdom
facility: Southampton General Hospital
Study officials:
affiliation: University of Padua and Istituto Oncologico Veneto
name: Valentina Guarneri, Prof
role: STUDY_CHAIR
References: