Clinical trial NCT01265017: Role of Pregnancy Related Hormones in Lowering the Insulin Requirement in Pregnant Women With Type 1 Diabetes
Official title: Single-center, Double-masked, Placebo-controlled Parallel-group Study of Pregnancy-related Hormones Estradiol and Medroxyprogesterone, in Conjunction With Hydrocortisone and Growth Hormone to Stimulate C-peptide Secretion in Women With T1DM
Study type: INTERVENTIONAL
Overall status: WITHDRAWN
Phase: PHASE1
Conditions: Type 1 Diabetes
Keywords: type 1 diabetes
pregnancy
insulin requirement
Lead sponsor: Sansum Diabetes Research Institute
Sponsor class: OTHER
First posted: 2010-12-22
Enrollment: 0
Sex: FEMALE
Minimum age (years): 18
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: PARALLEL
Primary purpose: BASIC_SCIENCE

Brief summary: This is a randomized, double-masked, placebo-controlled, single-center study to evaluate stimulated C-peptide secretion after exogenous administration of mild immunosuppression and growth-promoting factors to women with preexisting T1DM who had a decline in insulin requirement or had detectable C-peptide during a previous pregnancy. Fifteen subjects will be enrolled and randomly assigned in a 2:1 ratio to either active treatment or placebo in a parallel group design.

Participation for individual subjects will consist of an initial Screening Visit, a 2-week baseline period, a Baseline Visit, visits at week 2 and 4 of the treatment period, a visit at the end of the treatment period (week 6), and a follow-up visit 2 weeks after study treatment discontinuation.

Subjects will receive either active treatment or matching placebo of estradiol 1 mg every 8 hours; medroxyprogesterone 2.5 mg every 24 hours; hydrocortisone 2.5 mg every morning, 1.25 mg every afternoon, and 1.25 mg at bedtime each night; and growth hormone 2 mg once a day).

Detailed description: The primary objective of this study is to determine whether women with preexisting T1DM who showed a decline in insulin requirement, defined as a decrease in insulin requirement of 25% or more, or a decrease deemed to be clinically significant by the investigator, with no other medically determined reason, or who had detectable C-peptide during a previous pregnancy will show a change in stimulated C peptide response when not pregnant and treated with exogenous pregnancy-related hormones and growth factors (Estradiol, medroxyprogesterone, hydrocortisone, GH) for 6 weeks.

The secondary objectives of this study are as follows:

* Determine whether the study treatment leads to a change in T1DM autoantibodies between Baseline and Week 6
* Determine the percentage of subjects experiencing a clinically significant decline in total daily insulin requirement at Week 6, defined as a 25% decrease from Baseline
* Descriptively evaluate the association between serum levels of growth hormone, cortisol, and prolactin and changes in C-peptide levels
* Evaluate the safety of administration of the study treatments compared with placebo, as measured by blood pressure, pulse, weight change, blood glucose, and adverse events (AEs)

Eligibility criteria: Inclusion Criteria:

* Female aged 18 years or older with T1DM and a documented history of at least one of the following:
* Decrease in insulin requirement with no other medically determined reason
* Detectable C-peptide
* Free of systemic corticosteroid use within 3 months before study entry.
* Stable weight (±10%)
* Stable diet and exercise
* Stable insulin requirement (\<20% variability in insulin does in the 2 weeks prior to screening)Normal renal function as measured by an estimated glomerular filtration rate (simple MDRD)
* Negative pregnancy test and not planning to become pregnant during the study period. The subject must be willing to use an effective nonhormonal method of birth control during the study.
* Able to provide written informed consent.

Exclusion Criteria:

* Diagnosis of type 2 diabetes.
* Abnormal thyroid function (thyroid-stimulating hormone \[TSH\] and free thyroxine \[FT4\] test results) defined as TSH \<0.4mIU/L or TSH\>4.5mIU/L or Free T4 \<0.6ng/dL or Free T4\>1.6ng/dL.
* Abnormal renal function, as defined by serum creatinine greater than 1.2 mg/dL
* Any medical condition that, in the opinion of the investigator, yields the subject not suitable for study participation, including history of stroke, cancer, hypercoagulable problems, risk for deep vein thrombosis, and other unstable hormonal conditions, or Addison's disease or growth hormone deficiency.
* Currently treated with systemic steroids, hydrocortisone, growth hormone, or immunomodulatory medications
* Currently lactating.
* Pregnant within the last 9 months.
* Menopausal
* Taking hormonal therapy
* Known hypersensitivity to any of the medications used in this study or any component of the formulation.
* Known eating disorder
* History of phlebitis

Primary outcomes:
measure: stimulated C-peptide response
timeFrame: 6 weeks
Secondary outcomes:
measure: Clinical, immunologic and hormonal responses
timeFrame: 6 weeks
measure: Insulin requirement
timeFrame: 6 weeks
Other outcomes:
Locations:
city: Santa Barbara
country: United States
facility: Sansum Diabetes Research Institute
state: California
Study officials:
affiliation: Sansum Diabetes Research Institute
name: Lois Jovanovic, MD
role: PRINCIPAL_INVESTIGATOR
References: