Clinical trial NCT01866215: Effects of Exercise on Fructose Metabolism
Official title: Effects of Exercise on Fructose Metabolism
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Healthy Subjects
Keywords: gluconeogenesis
de novo lipogenesis
intramyocellular lipids
muscle glycogen
fructose
Lead sponsor: University of Lausanne
Sponsor class: OTHER
Collaborators: name: University of Bern
name: Centre Hospitalier Universitaire Vaudois
First posted: 2013-05-31
Enrollment: 8
Sex: MALE
Minimum age (years): 18
Maximum age (years): 40
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: CROSSOVER
Primary purpose: BASIC_SCIENCE

Brief summary: A high fructose diet increases fasting and post-prandial triglyceride (TG) concentrations in sedentary healthy human subjects.These effects may be secondary to fructose-induced hepatic de novo lipogenesis. Recent evidence indicate that exercise can prevent fructose induced dyslipidemia.This study will evaluate

1. how exercise effects the metabolic fate of oral fructose 1a) when exercise is performed before fructose ingestion 1b) when exercise is performed after fructose ingestion Metabolic effects of exercise will be assessed in healthy male subjects by measuring fructose oxidation (13CO2 production), fructose conversion into glucose (13C glucose concentrations in blood) and hepatic fructose conversion into lipid (13C palmitate-very low density lipoprotein (VLDL) concentrations in blood) after ingestion of 13C-labelled fructose meals
2. how fructose and protein modulate muscle glycogen and intramyocellular lipid repletion after exercise Healthy male subjects will be fed various fructose, glucose, lipid and whey protein meals after a glycogen/intramyocellular lipid depleting exercise. The effects of meals' composition will be assessed after 24 hours by measuring intramyocellular lipids and glycogen using proton-magnetic resonance spectroscopy (MRS).


Eligibility criteria: Inclusion Criteria:

* males
* 18-40 years old
* 19 kg/m2\>BMI\>25kg/m2
* moderate to high usual physical activity
* non-smokers

Exclusion Criteria:

* family history of diabetes mellitus
* ECG anomaly
* any medication
* participation to blood spending or other clinical study in the 3 months before the beginning of this study
* consumption of drugs
* consumption of more than 50g alcool/week
* family history of food intolerance

Primary outcomes:
measure: Plasma triglyceride
timeFrame: measurements in fasting conditions and every hour from 7:00 am to 4:00 pm (up to 9 hours)
measure: intramyocellular lipids
timeFrame: after glycogen/intramyocellular lipid depleting exercise and after 24 hour controlled feeding post exercise
measure: Fructose conversion into lipids
timeFrame: measured from 7:00 am to 4:00 pm in study 1 (up to 9 hours)
Secondary outcomes:
measure: Fructose conversion into glucose
timeFrame: measured between 7:00 am and 4:oo pm (up to 9 hours)
measure: intramyocellular glycogen concentrations
timeFrame: after glycogen/intramyocellular lipid depleting exercise and after 24 hour controlled feeding post exercise
measure: fructose oxidation
timeFrame: measured from 7:00 am to 4:00 pm in study 1 (up to 9 hours)
measure: exogenous lipid oxidation
timeFrame: measured from 7:00 am to 4:00 pm in study 2 (up to 9 hours)
Other outcomes:
Locations:
city: Lausanne
country: Switzerland
facility: Centre Hospitalier Universitaire Vaudois
state: Vaud
Study officials:
affiliation: University of Lausanne
name: Luc Tappy, MD
role: PRINCIPAL_INVESTIGATOR
References:
citation: Rosset R, Lecoultre V, Egli L, Cros J, Dokumaci AS, Zwygart K, Boesch C, Kreis R, Schneiter P, Tappy L. Postexercise repletion of muscle energy stores with fructose or glucose in mixed meals. Am J Clin Nutr. 2017 Mar;105(3):609-617. doi: 10.3945/ajcn.116.138214. Epub 2017 Jan 18.
citation: Egli L, Lecoultre V, Cros J, Rosset R, Marques AS, Schneiter P, Hodson L, Gabert L, Laville M, Tappy L. Exercise performed immediately after fructose ingestion enhances fructose oxidation and suppresses fructose storage. Am J Clin Nutr. 2016 Feb;103(2):348-55. doi: 10.3945/ajcn.115.116988. Epub 2015 Dec 23.