Clinical trial NCT02082600: Effects of Sleep Deprivation on Blood Hormones and Inflammatory Status After Exercise Induced-muscle Damage
Official title: Effects of Sleep Deprivation on Blood Hormones and Inflammatory Status After Exercise Induced-muscle Damage
Study type: INTERVENTIONAL
Overall status: UNKNOWN
Phase: NA
Conditions: Sleep
Sleep Deprivation
Lead sponsor: Federal University of São Paulo
Sponsor class: OTHER
Collaborators: name: Fundação de Amparo à Pesquisa do Estado de São Paulo
First posted: 2014-03-10
Enrollment: 10
Sex: MALE
Minimum age (years): 20
Maximum age (years): 31
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: CROSSOVER
Primary purpose: BASIC_SCIENCE

Brief summary: The aims of this study are examine the effects of sleep deprivation in muscle recovery after a maximum eccentric resistance exercise session performed on an isokinetic dynamometer (24 series of 10 repetitions). The sample will consist of 10 men, sedentary, clinically healthy, aged between 20 and 31 years old. Two experimental groups will be developed: EXE-SLEEP, in which subjects will perform the exercise protocol (\~18:00-19:00) and will be subject to normal period of sleep for 3 nights; EXE-TOTAL, in which subjects will perform the same exercise protocol and will be sleep deprived for 60 hours, followed by one night of sleep rebound.

Detailed description: The blood parameters analyzed will be:

* Creatine kinase and myoglobin: before, immediately after, 2, 4, 12, 24, 36, 48, and 60 hours after exercise protocol.
* Total and free testosterone, IGF-1, and cortisol: before, immediately after, 2, 4, 12, 24, 36, 48, and 60 hours after exercise protocol. Moreover, from 19:00 of the second day to 19:00 of the third day it will be collected blood samples every 2 hours.
* GH: during the second night, GH will be evaluated every 1 hour, from 23:00 to 07:00.
* TNF-alfa, IL-6, IL-1 beta, IL-10, and IL-1ra: before, immediately after, and 2 hour after exercise, as well every 4 hours from 19:00 of the second day to 19:00 of the third day.

It will be evaluated isometric strength every 12 hours, as well visual analog scale of pain.

Eligibility criteria: Inclusion Criteria:

* healthy subjects
* sedentary
* sleep duration between 6-8 hours peer night

Exclusion Criteria:

* smokers
* use vitamin supplements, like C and E
* drink two doses of alcohol/day or \> 4 doses in one occasion
* use of anti-inflammatory drugs
* antilipidemic drugs
* anemia
* muscle or joint diseases
* cardiovascular diseases
* diabetes
* renal diseases
* sleep disturbances
* breathing disorders

Primary outcomes:
measure: Change from baseline in isometric strength
timeFrame: Before exercise, immediatly after, 12, 24, 36, 48, and 60 hours after exercise induced-muscle damage
Secondary outcomes:
measure: Area Under Curve for serum total and free testosterone, IGF-1, and cortisol
timeFrame: Blood samples will be collected every 2 hours, for 24 hours.
Other outcomes:
measure: Area Under Curve for serum pro and anti-inflammatory cytokines
timeFrame: Blood samples will be collected every 4 hours, for 24 hours.
Locations:
city: Sao Paulo
country: Brazil
facility: Associação Fundo de Incentivo à Pesquisa
Study officials:
affiliation: Federal University of São Paulo
name: Marco Tulio Mello, PhD
role: PRINCIPAL_INVESTIGATOR
References: