Clinical trial NCT05844215: MMP-9, TIMP-9 in Lung Imaging and Functional of COVID-19
Official title: Role of MMP-9, TIMP-1 as Markers of Lung Imaging and Functional Abnormality of COVID-19
Study type: OBSERVATIONAL
Overall status: COMPLETED
Conditions: Pulmonary Infection
COVID-19
Extracellular Matrix Alteration
Lead sponsor: Universitas Airlangga
Sponsor class: OTHER
First posted: 2023-05-06
Enrollment: 78
Sex: ALL
Minimum age (years): 21
Maximum age (years): 70
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: no
Observational model: COHORT
Time perspective: PROSPECTIVE
Sampling method: NON_PROBABILITY_SAMPLE
Study population: Adult hospitalized COVID-19 patients in Universitas Airlangga Surabaya, Indonesia, proven by positive swab RT-PCR COVID-19.

Brief summary: This study aims to determine the role of the extracellular matrix in lung abnormalities in COVID-19 patients. Anatomical abnormalities of the lungs can be observed by the presence of abnormalities on a chest x-ray that is scored using the Brixia index. The presence of COVID-19 pneumonia can impact oxygenation disorders. It is hoped that knowing the relationship between biomarkers that affect the extracellular matrix and anatomical and functional abnormalities can open up new insights into new therapeutic opportunities. The balance of MMP-9 and TIMP-1 has been studied in relation to several lung diseases other than COVID-19.

Detailed description: Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV2), which has different degrees of severity, is the cause of Coronavirus Disease 2019 (COVID-19). Over 97% of COVID-19 patients make a full recovery. Inflammation increases when COVID-19 severity increases. The lungs and endothelium will suffer harm when different pro-inflammatory cytokines are released. A cytokine storm is started when many immune cells produce cytokines and chemokines. The pro-inflammatory cytokine can increase angiogenesis, vascular permeability, keratin proliferation, and collagen production. Lung lesions are affected by certain disorders. In COVID-19 patients, angiogenesis, fibroblast activation, and collagen deposition all contribute to the repair of the lungs. The pathological outcome of acute or chronic interstitial pulmonary disease is lung fibrosis, characterized by abnormal collagen and extracellular matrix (ECM) deposition, the persistence of fibroblasts, failure of alveolar re-epithelization, and destruction of normal pulmonary architecture. The breakdown or buildup of ECM is influenced by the ratio of matrix metalloproteinase (MMP) and tissue inhibitor matrix metalloproteinase (TIMP). TIMP distribution, not MMP distribution, was the primary contributor to lung fibrosis. MMP expression is increased by the production of several hormones, cytokines (IL-1, IL-6), growth factors (TGF, TNF-), platelet-derived growth factor (PDGF), and basic fibroblast growth factor (bFGF).

ECM assistance is required for the alveoli to function normally. Excessive ECM breakdown and aberrant ECM remodelling are the root causes of many respiratory conditions, including pulmonary fibrosis. ECM buildup destroys the alveolar process, particularly by impairing alveolar-capillary diffusion. On radiological images, lung abnormalities can be seen as pneumonia in the bilateral ground-glass opacity basal, which results in hypoxia. In the lower portion, bilateral abnormalities predominated. COVID-19 ARDS, hypoxemia, bilateral infiltrates, and reduced lung compliance were the hallmarks of pneumonia type H. Despite the limited sensitivity, plain chest X-rays can detect lung abnormalities in COVID-19; they are inexpensive and simple. New infiltrates on chest radiographs were used to make the diagnosis of pneumonia.

Biomarkers of pulmonary interstitial abnormalities have received limited attention in the literature, including investigations on MMP and TIMP for lung abnormalities in COVID-19 patients. One of the MMP gelatinase family's more complicated forms, MMP-9, can break down ECM components. MMP-9 increases collagen and cytokine production, fibroblast migration, and TGF- stimulation. MMP-9 is among the MMPs that TIMP1 effectively inhibits. TIMP-1 binds to pro- or latent MMP-9. For ECM proteolysis to occur, MMP and TIMP must coexist in equilibrium. This study aimed to ascertain how MMP-9 and TIMP-1 affected abnormal chest X-rays, poor oxygenation, severity, and death.

Blood is taken from hospitalized COVID-19 patients to measure the serum concentrations of MMP-9 and TIMP-1. At the time of hospital admission, three reviewers assessed the severity after chest X-Rays, followed by a BRIXIA score. At the time of hospital discharge, patients were evaluated. The levels of MMP-9 and TIMP-1 were compared to Brixia scores, oxygen consumption, severity, and death.

Eligibility criteria: Inclusion Criteria:

* The patient is infected with COVID-19 with positive RT-PCR Swab results
* Age 21-70 years
* Men or Women
* Various degrees of severity (mild, moderate, severe, critical) and comorbidities
* the Chest X-ray was done
* Willing to participate in research (signing informed consent)

Exclusion Criteria:

* The patient is TB active or has been treated for pulmonary TB
* Patients with a history of ILD clinically
* Patients with a history of asthma or COPD
* Pregnant women
* HIV/AIDS patients

Primary outcomes:
measure: Anatomical abnormalities of the lungs.
timeFrame: Chest X-Ray checked 2-4 hours during admission in emergency room.
measure: Functional abnormalities of the lungs.
timeFrame: Oxygen use and blood gas analysis checked 1-2 hours during admission in emergency room.
Secondary outcomes:
measure: Severity
timeFrame: Severity measured when patient hospitalized at first time during inclusion of study (4-6 hours after admission).
measure: Mortality
timeFrame: Mortality measured when patient out from hospital during the study (end of study).
Other outcomes:
Locations:
city: Surabaya
country: Indonesia
facility: Universitas Airlangga Hospital
state: East Java
Study officials:
affiliation: Airlangga University Faculty of Medicine: Universitas Airlangga Fakultas Kedokteran
name: Muhammad Amin, Prof.
role: PRINCIPAL_INVESTIGATOR
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