Clinical trial NCT06677749: Skeletal Muscle Myokine Response to Acute Eccentric Exercise
Official title: Skeletal Muscle Myokine Response to Acute Eccentric Exercise
Study type: INTERVENTIONAL
Overall status: RECRUITING
Phase: NA
Conditions: Skeletal Muscle
Lead sponsor: University of Manitoba
Sponsor class: OTHER
First posted: 2024-11-07
Enrollment: 12
Sex: ALL
Minimum age (years): 18
Maximum age (years): 35
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: NA
Intervention model: SINGLE_GROUP
Primary purpose: BASIC_SCIENCE

Brief summary: The purpose of this study is to evaluate the acute local skeletal muscle inflammatory response to thigh muscle (quadriceps) eccentric muscle actions. Eccentric muscle actions occur when the muscle is lengthening as it contracts. This is a pilot project to assess the degree of inflammatory biomarkers, called myokines, that are released locally in skeletal muscle tissue after an acute bout of resistance-exercise using a resistance-exercise machine called an isokinetic dynamometer.

Detailed description: The intervention will involve one session of maximal eccentric exercise of the knee extensor muscles (quadriceps) of the right leg. During the 24-hour period preceding the intervention, participants will be asked to refrain from any for of moderate-vigorous physical activity or exercise. Participants will perform 30 sets of 10 repetitions of maximal isokinetic eccentric knee extensions at 60 degrees/second with 1-minute of rest between each set. Before completing the exercise bout, each participant will be asked to remain in a seated position (resting) for 30-minutes and then provide a skeletal muscle biopsy using the modified Bergstrom with suction muscle biopsy technique from the vastus lateralis on the right leg. This will be done for the baseline assessment of 5 myokines including apelin, fibroblast growth factor-21, irisin, interleukin-6, and interleukin-15. Then the participants will complete the exercise bout as described above. They will then rest quietly in the laboratory for 3-hours and wait for the second skeletal muscle biopsy to be taken following this 3-hour wait.

Eligibility criteria: Inclusion Criteria:

* 18-35 years old
* healthy
* recreationally active (\>/= 150 minutes of moderate to vigorous physical activity per week for at least the past three months)

Exclusion Criteria:

* "yes" answer to the Get Active Questionnaire which screens for health conditions that may affect exercise ability

Primary outcomes:
measure: Apelin
timeFrame: Baseline and 3-hours post-exercise
measure: Fibroblast growth factor-21
timeFrame: Baseline and 3-hours post-exercise
measure: Irisin
timeFrame: Baseline and 3-hours post-exercise
measure: Interleukin-6
timeFrame: Baseline and 3-hours post-exercise
measure: Interleukin-15
timeFrame: Baseline and 3-hours post-exercise
Secondary outcomes:
measure: Body mass
timeFrame: Baseline
measure: Body height
timeFrame: Baseline
measure: Body mass index
timeFrame: Baseline
measure: Fat mass
timeFrame: Baseline
measure: Fat free mass
timeFrame: Baseline
Other outcomes:
Locations:
city: Winnipeg
country: Canada
facility: Applied Research Centre, Faculty of Kinesiology, University of Manitoba
state: Manitoba
Study officials:
affiliation: University of Manitoba
name: Stephen M Cornish, PhD
role: PRINCIPAL_INVESTIGATOR
References: