Clinical trial NCT04469348: Inflammation, Intracellular Invasion and Colonization of the Nasal Mucosa by Staphylococcus Aureus
Official title: Inflammation, Intracellular Invasion and Colonization of the Nasal Mucosa by Staphylococcus Aureus
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Healthy
Keywords: Staphylococcus aureus
ICAM-1
nasal cells
S. aureus
nasal swab
Lead sponsor: Centre Hospitalier Universitaire de Saint Etienne
Sponsor class: OTHER
First posted: 2020-07-14
Enrollment: 157
Sex: ALL
Minimum age (years): 18
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: yes
Allocation: NA
Intervention model: SINGLE_GROUP
Primary purpose: DIAGNOSTIC

Brief summary: An inflammatory state of the nasal cells (very close to keratinocytes) could favour the internalization of Staphylococcus aureus and thus constitute a persistent reservoir for the carriage of this bacterium.

Staphylococcus aureus is a commensal bacterium of the skin and mucous membranes that colonizes approximately 2 billion people worldwide Staphylococcus aureus is also a leading cause of community and healthcare-associated infection. Staphylococcus aureus has demonstrated its ability to invade many non-professional phagocytic cell lines such as keratinocytes, osteoblasts, fibroblasts, epithelial cells and endothelial cells. During pro-inflammatory stimulation, internalization of Staphylococcus aureus into keratinocytes is mainly mediated by ICAM-1. These results suggest that, in humans, an inflammatory state of the nasal cells (very close to keratinocytes) could promote the internalization of Staphylococcus aureus and thus constitute a persistent reservoir for the carriage of this bacterium.

Detailed description: The main objective is to compare the expression level of ICAM-1 in nasal cells with and without the presence of Staphylococcus aureus.

Eligibility criteria: Inclusion Criteria:

* Subject affiliated to a social security scheme or entitled person
* Subject who received informed information about the study et signed consent
* Adult subject (≥18ans)
* Healthy Volunteer

Exclusion Criteria:

* Subject undergoing antibiotic treatment or having received antibiotic treatment (oral, IV or local) in the month prior to inclusion
* Subject with a major haemostasis disorder or anticoagulant treatment contraindicated for surgery
* Subject with a pathology increasing the nasal carriage of S. aureus (chronic furunculosis, diabetes, HIV infection, chronic rhinosinusitis)
* Subject under guardianship or trusteeship
* Pregnant woman

Primary outcomes:
measure: level of ICAM-1 expression in nasal cells
timeFrame: Months: 0
Secondary outcomes:
measure: Measurement of the expression of the genes (mRNA dosage) involved in IL-1 beta secretion
timeFrame: Months: 0
measure: Measurement of the expression of the genes (mRNA dosage) involved in activation of autophagy
timeFrame: Months: 0
measure: cytokine concentration
timeFrame: Months: 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
measure: concentration of ICAM-1 in S. Aureus
timeFrame: Months: 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
Other outcomes:
Locations:
city: Saint-Étienne
country: France
facility: CHU de Saint-Etienne
Study officials:
affiliation: CHU SAINT-ETIENNE
name: Paul Verhoeven, MD
role: PRINCIPAL_INVESTIGATOR
References: