Clinical trial NCT02070848: Muscle Blood Flow During Exercise
Official title: Muscle Blood Flow Control During Exercise in Humans: Contribution From Mechanical and Metabolic Signals and Role of the Erythrocyte.
Study type: OBSERVATIONAL
Overall status: COMPLETED
Conditions: Skeletal Muscle Blood Flow
Keywords: red blood cell
knee extension exercise
Lead sponsor: University Hospital, Strasbourg, France
Sponsor class: OTHER
First posted: 2014-02-25
Enrollment: 20
Sex: MALE
Minimum age (years): 18
Maximum age (years): 45
Standard ages: ADULT
Accepts healthy volunteers: yes
Observational model: CASE_ONLY
Time perspective: PROSPECTIVE
Sampling method: NON_PROBABILITY_SAMPLE
Study population: Subjects will be students recruited in the Faculty, sports clubs...

Brief summary: Physical exercise triggers various physiological responses including a marked increase in muscle blood flow and oxygen delivery in order to support muscle activity. How muscle blood flow is controlled is currently unclear.

The primary purpose of this study is to establish the respective contribution of metabolic (linked to energy requirements) and mechanical (linked to muscle tensions) signals in the exercise hyperemia and the possible role of the red blood cells.

Vasodilation and muscle blood flow during exercise are controlled by ATP released in the plasma from the red blood cells in response to a combination of metabolic and mechanical signals applied on the vasculature.


Eligibility criteria: Inclusion Criteria:

* Non smoker or having stopped smoking for at least 5 yr
* Practice sports: physical activity level will be evaluated with a questionnaire (de Baecke et coll., 1982) and will give an activity index greater than 6 together with a VO2max greater than 50 ml/kg/min
* Body mass index lower than 25
* Registered to the French Social Security system
* Providing a written and informed consent to participate in the study

Exclusion Criteria:

* History of venous or arterial thrombo-embolic events
* Bad perfusion from the cubital artery
* Unable to understand the information notice
* Subject exposed to law investigations or with financial dependancy
* Subjects feeling unwell whan seing blood.
* Subjects advised to not practice sport.
* Musculo-tendinous or articular issues with the lower leg
* Respiratory, cardiovascular or metabolic pathologies
* Current medical treatment that can not be stopped 7 days before the experiment.

Primary outcomes:
measure: Change in leg blood flow during concentric and eccentric muscle work
timeFrame: Leg blood flow measurements during exercises performed at the beginning, after 14 minutes, then after 18 min, 22 min, 26 min, 30 min, 33 min, 35 min and 45 min
Secondary outcomes:
Other outcomes:
Locations:
city: Strasbourg
country: France
facility: SERVICE DE PHYSIOLOGIE ET D'EXPLORATIONS FONCTIONNELLES - Nouvel Hôpital Civil
Study officials:
affiliation: Hôpitaux Universitaires de Strasbourg
name: Stéphane DOUTRELEAU, MD
role: PRINCIPAL_INVESTIGATOR
affiliation: Faculté des Sciences du Sport 14 Rue René Descartes 67084 Strasbourg
name: DUFOUR Stéphane, MD
role: STUDY_DIRECTOR
affiliation: Faculté des Sciences du Sport 14 Rue René Descartes 67084 Strasbourg
name: FAVRET Fabrice, MD
role: STUDY_CHAIR
affiliation: Hôpitaux Universitaires de Strasbourg
name: LONSDORFER Evelyne, MD
role: PRINCIPAL_INVESTIGATOR
References: