Clinical trial NCT03087500: Physiological Abnormalities Associated with Down Syndrome
Official title: Physiologic Biomarkers Within the Functional Spectrum of Down Syndrome
Study type: OBSERVATIONAL
Overall status: WITHDRAWN
Conditions: Down Syndrome
Keywords: Down Syndrome
Typically Developing Participants
Children
Adolescents
Adults
Lead sponsor: University of Arkansas
Sponsor class: OTHER
Collaborators: name: Down Syndrome Research Foundation
name: Arkansas Children Research Institute
First posted: 2017-03-22
Enrollment: 0
Sex: ALL
Minimum age (years): 3
Maximum age (years): 50
Standard ages: CHILD
ADULT
Accepts healthy volunteers: no
Observational model: CASE_CONTROL
Time perspective: PROSPECTIVE
Sampling method: NON_PROBABILITY_SAMPLE
Study population: Downs Syndrome: 120 clinically confirmed full trisomy 21, age 3-50 years of both sexes will be recruited as the target study population. Half of the individuals (n=60) will be children (3-17 years of age) while half (n=60) will be adults (18-50 years of age) Control Subjects: 60 typically developing individuals age 3-50, age and gender matched to at least one participant with DS. Half of the controls (n=30) will be age and gender match to children with DS and half (n=30) will be age and gender matched to adults with DS.

Brief summary: The overall goal of this study is to evaluate biomarkers of oxidative stress, mitochondrial function, and DNA methylation (epigenetics) in order to determine the extent to which these biomarkers are related to cognitive, behavioral and adaptive function in Down Syndrome. The inter-relationship between measurable biomarkers and functional/cognitive abilities will move beyond genetics to provide unprecedented new knowledge and a broader understanding of the underlying pathophysiology and abnormal gene expression induced by trisomy 21.

Detailed description: The Investigators preliminary evidence indicates that people with DS have metabolic biomarkers associated with oxidative stress (GSH/GSSG) and reduced methylation capacity (SAM/SAH) as well as abnormal DNA methylation (epigenetics). The investigative team hypothesize that these abnormal metabolic processes contribute to abnormalities in behavior and development associated with trisomy 21; this connection has never been investigated. Confirming and expanding on the preliminary data would provide new understanding of the biological and functional etiology of the behavioral and developmental delays associated with Trisomy 21. Further, establishing the underlying relationship between metabolic abnormalities and behavioral/cognitive function over the age spectrum can provide strong support for the design of future treatments of individuals with DS aimed at improving their behavior and development. In addition, these biomarkers may also prove to be predictive biomarkers for the risk of developing ASD like behaviors or Alzheimer's disease in this population. Finally, examining the modulating role of diet in the severity of biological abnormalities will provide new information for lifestyle guidance to improve biomarkers and potentially minimize the medical co-morbidities associated with trisomy 21.

Eligibility criteria: Inclusion Criteria:

1. Participant or guardian ability to consent/assent and willing to comply with protocol requirements

Exclusion Criteria:

1. Trisomy translocation or mosaics.
2. Untreated hypothyroidism
3. Known history of liver disease, renal disease, Hepatitis B or C or HIV
4. Recent infection with fever or requiring hospitalization within past 30 days.
5. Any medical condition, use of medications, nutrient or herbal supplements that would interfere with the study results as determined by the PI
6. Chemotherapy
7. Recent surgery (within 2 months)
8. Untreated Epilepsy
9. Any chronic medical/behavioral condition and/or treatments that may interfere with study related outcomes, as determined by PI
10. Dementia
11. History of a significant adverse reaction to a prior blood draw
12. Any other historical event/information that may, in the opinion of the PI, be a reason to exclude the child from participation.

Primary outcomes:
measure: Microbiome Analysis
timeFrame: 2 years
measure: Mitochondrial Function Analysis
timeFrame: 2 years
measure: Oxidative Stress Analysis
timeFrame: 2 years
measure: Immune Function
timeFrame: 2 years
measure: Metabolomics
timeFrame: 2 years
measure: Epigenetics
timeFrame: 2 years
measure: Folate Receptor Alpha Autoantibody (FRAA)
timeFrame: 2 years
measure: Thyroid Function
timeFrame: 2 years
measure: Diet
timeFrame: 2 years
Secondary outcomes:
Other outcomes:
Locations:
Study officials:
affiliation: Arkansas Childrens Research Institute
name: Richard Frye
role: PRINCIPAL_INVESTIGATOR
References: