Clinical trial NCT02882386: Amino Acid Kinetics in Blood After Consuming Different Milk Protein Supplements
Official title: Does Native Whey Induce Greater Blood Leucine Concentrations Than Other Whey Protein Supplements and Milk: A Randomized Controlled Trial?
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Healthy Participants
Lead sponsor: Norwegian School of Sport Sciences
Sponsor class: OTHER
Collaborators: name: Arkansas Children's Hospital Research Institute
name: Tine
First posted: 2016-08-29
Enrollment: 13
Sex: MALE
Minimum age (years): 18
Maximum age (years): 45
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: CROSSOVER
Primary purpose: BASIC_SCIENCE

Brief summary: The aim of this study is to investigate the amino acid kinetics in blood after a bout of strength training and ingestion of different milk protein supplements (native whey, whey protein concentrate 80, hydrolysed whey, microparticulated whey and milk) The investigators hypothesize that native whey will give a faster and higher rise in blood concentrations of leucine compared to the other milk protein supplements.

Detailed description: Increasing or maintaining muscle mass is of great importance for populations ranging from athletes to patients and elderly. Resistance exercise and protein ingestion are two of the most potent stimulators of muscle protein synthesis. Both the physical characteristic of proteins (e.g. different digestion rates of whey and casein) and the amino acid composition, affects the potential of a certain protein to stimulate muscle protein synthesis. Given its superior ability to rapidly increase blood leucine concentrations to high levels, whey is often considered the most potent protein source to stimulate muscle protein synthesis. Native whey protein is produced by filtration of unprocessed milk. Consequently, native whey has different characteristics than WPC-80, which is exposed to heating and acidification. Because of the direct filtration of unprocessed milk, native whey is a more intact protein compared with WPC-80. Of special interest is the higher amounts of leucine in native whey.

The aim of this double-blinded randomized 5-arm cross-over study is to compare amino acid kinetics in blood after a bout of strength training and ingestion of 20 grams of high quality, but distinct, dairy protein supplements (native whey, whey protein concentrate 80, hydrolysed whey, microparticulated whey and milk). Furthermore, the investigators investigate whether differences in amino acid kinetics affect acute blood glucose and urea response, as well as recovery of muscle function after a bout of strength training.

The investigators hypothesize that native whey will give a faster and higher rise in blood concentrations of leucine compared to the other protein supplements.

Eligibility criteria: Inclusion Criteria:

* Healthy in the sense that they can conduct training and testing
* Able to understand Norwegian language written and oral

Exclusion Criteria:

* Diseases or injuries contraindicating participation
* Use of dietary supplements (e.g. proteins, vitamins and creatine)
* Lactose intolerance
* Allergy to milk
* Allergy towards local anesthetics (xylocain)

Primary outcomes:
measure: Plasma amino acid concentration change from baseline
timeFrame: Blood collected at 0, 30, 45, 60, 90 and 120 min after consumption of protein supplements
Secondary outcomes:
measure: Serum glucose change from baseline
timeFrame: Blood collected at 0, 30, 45, 60, 90 and 120 min after consumption of protein supplements
measure: Serum urea change from baseline
timeFrame: Blood collected at 0, 30, 45, 60, 90 and 120 min after consumption of protein supplements. For milk and native whey blood was collected at two additional time points: 22 and 30 hours after consumption of protein supplements
measure: Muscle force generating capacity change from baseline
timeFrame: Measured before and at 0, 6, 22 and 30 hours after exercise. Only measured after milk and native whey
measure: Jump height change from baseline
timeFrame: Measured before and at 0, 6, 22 and 30 hours after exercise. Only measured after milk and native whey
measure: Serum creatine kinase change from baseline
timeFrame: Blood collected at 0, 30, 45, 60, 90 and 120 min after consumption of protein supplements. For milk and native whey blood was collected at two additional time points: 22 and 30 hours after consumption of protein supplements
measure: Muscle soreness change from baseline
timeFrame: Measured before and at 0, 6, 22 and 30 hours after exercise. Only measured after milk and native whey
Other outcomes:
Locations:
Study officials:
affiliation: Norwegian School of Sport Sciences
name: Truls Raastad, PhD
role: PRINCIPAL_INVESTIGATOR
References: