Clinical trial NCT00275886: Activation and Recruitment of GIL During Hp Infection
Official title: Activation and Recruitment of Gastric Infiltrating Lymphocytes During H. Pylori Infection
Study type: OBSERVATIONAL
Overall status: UNKNOWN
Conditions: Helicobacter Gastritis
Dyspepsia
Lead sponsor: National Taiwan University Hospital
Sponsor class: OTHER
First posted: 2006-01-12
Enrollment: 30
Sex: ALL
Minimum age (years): 20
Maximum age (years): 60
Standard ages: ADULT
Accepts healthy volunteers: no
Observational model: CASE_CONTROL
Time perspective: OTHER

Brief summary: Helicobacter pylori is a major human pathogen that infects over half of the world population. Infection initiates a series of changes in the gastric mucosa, beginning with gastritis and leading in some patients to peptic ulcer disease, mucosa-associated lymphomas, and gastric adenocarcinoma. It is believed that host factors, in particular, the T cell-mediated immune responses may play an important role in the pathogenesis of diseases induced by H. pylori infection. Recent results revealed that there were higher IFN-γ secreting cells in gastric infiltrating T cells isolated from H. pylori infected patients than in uninfected patients, suggesting that the TH1 response and degree of IFN-γ production is associated with disease severity. Meanwhile, recent studies have shown that apoptosis of the gastric epithelium is increased during infection and this response is associated with an expansion of gastric T-helper type 1 (Th1) cells. In this project, we are trying to further investigate role of host T cell mediated immune response in pathogenesis of Helicobacter infection by characterization of the expression of chemokine receptors on gastric infiltrating lymphocytes. We are going to investigate the mechanisms involving in chemokine/chemokine receptor interaction in recruitment of gastric infiltrating lymphocytes and pathogenesis of gastric mucosa damage in Helicobacter infection. This study will be helpful for understanding the mechanisms of activation and recruitment of gastric-infiltrating lymphocytes during gastric inflammation.

Detailed description: Specific Aim #1: Characterization of the gastric infiltrating T cells from H. pylori gastritis patients A. Patients H. pylori infected gastritis and asymptomatic patients are selected from National Taiwan University hospital. The gastric biopsy specimen are taken from endoscopic biopsy in routine health check up or panendoscopic examination for the despeptic patients. HP negative gastritis patients will also be studied for negative control.

B. Immunohistochemical identification of T cells and expression of chemokine receptors.

Specific Aim #2 . Isolation and characterization of chemokines and chemokine receptors of the infiltrating lymphocytes in H. pylori-associated gastritis tissue

A. Isolation of gastric infiltrating lymphocyte B. Identification of the expression of chemokine receptor in gastric infiltrating lymphocytes by flow cytometry C. Detection of expression of chemokines in gastric tissues

Eligibility criteria: Inclusion Criteria:

* patients receiveing endoscopy examination due to dyspepsia

Exclusion Criteria:

* nil

Primary outcomes:
Secondary outcomes:
Other outcomes:
Locations:
city: Taipei
country: Taiwan
facility: National Taiwan University Hospital
Study officials:
affiliation: Department of Immunology
name: Ping-Ning Hsu, MD, PhD
role: PRINCIPAL_INVESTIGATOR
References: