Clinical trial NCT00725166: Human Muscle Gene Expression Profiles in Young and Old Men
Official title: Human Muscle Gene Expression Profiles in Young and Old Men
Study type: OBSERVATIONAL
Overall status: COMPLETED
Conditions: Elderly
Sarcopenia
Keywords: Aging
Biopsy
Skeletal muscle
Molecular biology
Genetic markers
Gene expression regulation
Young and old men
Healthy volunteers
Lead sponsor: Centre Hospitalier Universitaire de Saint Etienne
Sponsor class: OTHER
Collaborators: name: Fondation des Caisses d'Epargne Rhône-Alpes
name: Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
First posted: 2008-07-30
Enrollment: 41
Sex: MALE
Minimum age (years): 19
Maximum age (years): 76
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: yes
Observational model: CASE_CONTROL
Time perspective: PROSPECTIVE
Sampling method: NON_PROBABILITY_SAMPLE
Study population: Young : students of the Saint-Etienne's university. Old : men who participate in the PROOF study

Brief summary: To reach the goals of living longer in better medical conditions, many countries reach the same conclusion: new strategies have to be developed to avoid, or at least limit, the effects of age; this requires a better knowledge of the mechanisms of aging. Our project focuses on the loss of muscle mass associated with aging, called sarcopenia. Sarcopenia unavoidably leads to impaired mobility and poor balance, which contributes to loss of functional autonomy and to increased prevalence for severe falls. Skeletal muscle also plays a central role as a reserve for energy and amino acids. Hence, sarcopenia further triggers severe side metabolic effects such as frailty among elderly persons. The precise mechanisms of muscle aging are still mostly unknown, although many theories have been proposed.

The present study aims at better understanding the mechanisms of skeletal muscle loss associated with aging. Using muscle biopsies from young and old subjects, the differential expression profiles of mRNA will be obtained through chips that will evaluate more than 39000 transcripts. On the same samples, proteomic analyses will involve two complementary approaches: (1) bidimensional electrophoresis (2DGE) coupled to mass spectrometry (MALDI-ToF) for dominant proteins; (2) Western-blot (more than 800 antibodies) targeting regulating proteins not detectable using 2DGE. Complementary histological studies (immunohisto-fluorescence, confocal microscopy) will specify the localisation of the major biomarkers in the muscle biopsies.

The results of that research will have applications in the medium term and will lead to nutritional interventions to modulate specific metabolic pathways and improve the quality of life in the elderly.


Eligibility criteria: Inclusion Criteria:

* Affiliated or beneficiary of a social security category
* Having signed the inform consent form
* Having signed the genetic consent form

Exclusion Criteria:

* Antiaggregant platelet treatment
* Type 2 diabetes
* Body Mass Index \> 25
* Severe renal disease
* CRP \> 10
* Abnormal clotting test
* Allergies to local anesthetics

Primary outcomes:
measure: To identify the differential expression profiles (proteomics, transcriptomics) in skeletal muscle between young and old men
timeFrame: During biopsie (W3)
Secondary outcomes:
measure: To specify the localisation of the major biomarkers in the muscle biopsies.
timeFrame: During biopsie (W3)
measure: To compare muscular energy metabolic enzymatic activities between young and old men
timeFrame: during biopsie (W3)
measure: To compare the number of muscular stem cells between young and old men
timeFrame: during biopsie (W3)
Other outcomes:
Locations:
city: Saint-etienne
country: France
facility: CHU de Saint-Etienne
Study officials:
affiliation: INRA Clermont-Ferrand
name: Daniel BECHET, PhD
role: STUDY_CHAIR
affiliation: CHU de Saint-Etienne
name: Jean-Claude BARTHELEMY, MD PhD
role: PRINCIPAL_INVESTIGATOR
References: