Clinical trial NCT02758470: Muscle Fatigue and Carbonic Anhydrase Inhibitors
Official title: The Effect of Carbonic Anhydrase Inhibitors on the Pulmonary System Response to Muscle Fatigue.
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: PHASE4
Conditions: Hypoxia
Keywords: Muscle Fatigue
Acetazolamide
Methazolamide
Lead sponsor: University of British Columbia
Sponsor class: OTHER
First posted: 2016-05-02
Enrollment: 13
Sex: MALE
Minimum age (years): 18
Maximum age (years): 40
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: CROSSOVER
Primary purpose: BASIC_SCIENCE

Brief summary: The objective of this research is to assess the effects of acetazolamide and methazolamide on respiratory and limb muscle fatigue development. A fatiguing protocol will be conducted for the respiratory and plantar flexor muscles and the difference in pressure/torque produced by supramaximal nerve stimulation used to assess muscle fatigue between conditions.


Eligibility criteria: Inclusion Criteria:

* Age: 18-40 years.
* Regularly physically active
* Male

Exclusion Criteria:

* current or ex-smokers
* pulmonary function \<80% of predicted
* esophageal tumour or ulcer
* have had recent (\<6 months) musculoskeletal injury or any surgery to the lower leg
* have contraindications to carbonic anhydrase inhibitors (eg. severe or absolute glaucoma, adrenocortical insufficiency, hepatic insufficiency, renal insufficiency, sulfa allergy or an electrolyte imbalance such as hyperchloremic acidosis)
* are obese (BMI \>30 Kg/m2)
* taking diuretics, blood thinners, or anti-platelet drugs.

Primary outcomes:
measure: Change in trans-diaphragmatic pressure generation to supramaximal phrenic nerve stimulation following inspiratory threshold loading.
timeFrame: Baseline, 1, 5, 10, 20, and 30 minutes following the loading protocol for each arm of the study
measure: Change in plantar flexor muscle group torque generation to supramaximal tibial nerve stimulation following an isometric loading protocol
timeFrame: Baseline, 1, 5, 10, 20, and 30 minutes following the loading protocol for each arm of the study
Secondary outcomes:
measure: Maximal inspiratory pressure maneuvers
timeFrame: Baseline and immediately following loading protocol
measure: Maximal Voluntary Contraction of the Plantar Flexor Muscle Group
timeFrame: Baseline and immediately following the loading protocol
measure: Contraction time and half-relaxation time
timeFrame: Baseline, 1, 5, 10, 20, and 30 minutes post loading protocol
Other outcomes:
measure: Esophageal and Gastric Pressure
timeFrame: Baseline, 1, 5, 10, 20, and 30 minutes post loading protocol
measure: Heart Rate
timeFrame: Baseline, 1, 5, 10, 20, and 30 minutes post loading protocol
measure: Beat-by-beat blood pressure
timeFrame: Baseline, 1, 5, 10, 20, and 30 minutes post loading protocol
Locations:
city: Kelowna
country: Canada
facility: University of British Columbia
state: British Columbia
Study officials:
affiliation: University of British Columbia
name: Glen E Foster, Ph.D.
role: PRINCIPAL_INVESTIGATOR
References:
citation: Dominelli PB, McNeil CJ, Vermeulen TD, Stuckless TJR, Brown CV, Dominelli GS, Swenson ER, Teppema LJ, Foster GE. Effect of acetazolamide and methazolamide on diaphragm and dorsiflexor fatigue: a randomized controlled trial. J Appl Physiol (1985). 2018 Sep 1;125(3):770-779. doi: 10.1152/japplphysiol.00256.2018. Epub 2018 May 24.