Clinical trial NCT03811717: Fasting and Exercise-induced Changes in Human Skeletal Muscle
Official title: Impact of Exercise-induced Energetic Stress During an 8-hour Fast on Metabolic Regulation in Resting Human Skeletal Muscle
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Skeletal Muscle Energetics
Lead sponsor: Queen's University
Sponsor class: OTHER
Collaborators: name: University of Waterloo
First posted: 2019-01-22
Enrollment: 10
Sex: MALE
Minimum age (years): 18
Maximum age (years): 30
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: CROSSOVER
Primary purpose: BASIC_SCIENCE

Brief summary: This study examines the impact of increasing energetic stress during an 8-hour fast on metabolic pathways in human skeletal muscle. Specifically, participants will undergo two 8-hour fasting sessions in a randomized crossover fashion, with the addition of low-intensity arm exercise in one of the sessions. Changes in metabolic pathways will be examined in resting (leg) muscle before, during, and after the fast.


Eligibility criteria: Inclusion Criteria:

* recreationally active (no more than 3 hours of weekly physical activity)
* not currently involved in a systematic training program
* body mass index \< 30 kg/m2

Exclusion Criteria:

* presence of cardiometabolic disease
* taking regular oral medication
* current smoker

Primary outcomes:
measure: Peroxisome proliferator activated receptor gamma co-activator 1-alpha (PGC-1α) messenger ribonucleic acid (mRNA) expression
timeFrame: 15 min before fast; after 4 and 8 hours of fasting
Secondary outcomes:
measure: Catecholamine concentrations
timeFrame: 15 min before fast; after exercise; after 4 and 8 hours of fasting
measure: Glucose and insulin concentrations
timeFrame: 15 min before fast; after 4 and 8 hours of fasting
measure: total and phosphorylated AMP activated protein kinase (AMPK)
timeFrame: 15 min before fast; after 4 and 8 hours of fasting
measure: total and phosphorylated SIRT-1
timeFrame: 15 min before fast; after 4 and 8 hours of fasting
measure: total and phosphorylated mammalian target of rapamycin (mTOR)
timeFrame: 15 min before fast; after 4 and 8 hours of fasting
measure: total and phosphorylated Akt/Protein Kinase B
timeFrame: 15 min before fast; after 4 and 8 hours of fasting
measure: total and phosphorylated acetyl coenzyme A carboxylase (ACC)
timeFrame: 15 min before fast; after 4 and 8 hours of fasting
measure: total and phosphorylated p38 mitogen activated protein kinase (MAPK)
timeFrame: 15 min before fast; after 4 and 8 hours of fasting
measure: phosphorylated protein kinase A (PKA) substrates
timeFrame: 15 min before fast; after 4 and 8 hours of fasting
measure: acetylated p53
timeFrame: 15 min before fast; after 4 and 8 hours of fasting
measure: Sirtuin 1 (SIRT1) mRNA expression
timeFrame: 15 min before fast; after 4 and 8 hours of fasting
measure: Sirtuin 3 (SIRT3) mRNA expression
timeFrame: 15 min before fast; after 4 and 8 hours of fasting
measure: Pyruvate dehydrogenase kinase isoenzyme 4 (PDK4) mRNA expression
timeFrame: 15 min before fast; after 4 and 8 hours of fasting
measure: p53 mRNA expression
timeFrame: 15 min before fast; after 4 and 8 hours of fasting
measure: Leucine rich pentatricopeptide repeat containing protein (LRP130) mRNA expression
timeFrame: 15 min before fast; after 4 and 8 hours of fasting
measure: General control non-repressible 5 (GCN5) mRNA expression
timeFrame: 15 min before fast; after 4 and 8 hours of fasting
measure: PGC-1α protein content
timeFrame: 15 min before fast; after 4 and 8 hours of fasting
measure: Cytochrome C oxidase subunit 4 (COX-IV) protein content
timeFrame: 15 min before fast; after 4 and 8 hours of fasting
measure: Intramuscular glycogen content
timeFrame: 15 min before fast; after 4 and 8 hours of fasting
measure: Intramuscular triglyceride (IMTG) content
timeFrame: 15 min before fast; after 4 and 8 hours of fasting
Other outcomes:
Locations:
city: Kingston
country: Canada
facility: School of Kinesiology and Health Studies
state: Ontario
Study officials:
affiliation: Queen's University
name: Brendon J. Gurd, PhD
role: PRINCIPAL_INVESTIGATOR
References: