Clinical trial NCT03985475: Identification of the Cutaneous Microbiota in Patients With Cutaneous Infection (MICROBIOTA)
Official title: Identification of the Cutaneous Microbiota in Patients With Cutaneous Infection Admitted in the Department of Acute Infectious Diseases
Study type: OBSERVATIONAL
Overall status: UNKNOWN
Conditions: Skin Infections
Lead sponsor: Assistance Publique Hopitaux De Marseille
Sponsor class: OTHER
First posted: 2019-06-13
Enrollment: 100
Sex: ALL
Minimum age (years): 18
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: no
Observational model: COHORT
Time perspective: PROSPECTIVE
Sampling method: PROBABILITY_SAMPLE
Study population: Any patient present meeting the inclusion criteria of the study will be offered to participate in the study.

Brief summary: Identify the cutaneous microbiota on a cutaneous lesion (cellulite, wound, rash, etc.) on a swab, biopsies or abscess puncture and on "healthy" skin on a skin swab performed for cutaneous mapping to search for staphylococcal deposits.

Detailed description: Cellulite is characterized by inflammation and an alleged infection of the skin and subcutaneous tissues. Previous studies have shown a low yield of samples such as biopsy and needle aspiration of the order of 16%. The most common pathogens are Staphylococcus aureus and Streptococcus pyogenes.

Conventional cultivation has limitations such as the lack of detection of fastidious microorganisms, or the use of antibiotics. Molecular diagnostic methods, such as detection of 16S ribosomal DNA followed by amplification and pyrosequencing, have been used to overcome the limitations of microbial culture.

In a recent study, the authors investigated the causes of acute cellulitis without performing drainage but skin biopsies from the infected site and another non-infected site by quantitative PCR, pyrosequencing, and conventional culture. PCR identified methicillin-resistant S. aureus methicillin in approximately 30% -40% of cases with similar frequency in infected and uninfected sites. In another study R. felis was found in not only bedsores, but also, swabs taken from healthy skin, as well as Streptococcus pneumoniae, S. aureus and Streptococcus group A. Being engaged in the exploration of the human microbiota especially by culture we propose to extend the knowledge of this skin microbiota in patients hospitalized in Infectious Disease services.

Eligibility criteria: Inclusion Criteria:

* Patient with skin infections at admission
* Patient requiring skin mapping
* Person informed of the study and having expressed no opposition to participate in the study.
* Affiliate or beneficiary of a social security

Exclusion Criteria:

* Subject not agreeing to participate
* Vulnerable person: pregnant, parturient or nursing woman, person under guardianship or curatorship, or deprived of liberty by a judicial or administrative decision.

Primary outcomes:
measure: Distribution of microorganisms found according to patient characteristics
timeFrame: 36 moths
Secondary outcomes:
Other outcomes:
Locations:
city: Marseille
country: France
facility: Service de maladies Infectieuses
state: Cedex 5
Study officials:
affiliation: AP HM
name: Jean-Olivier ARNAUD
role: STUDY_DIRECTOR
References: