Clinical trial NCT06890741: Inflamation, Gut Leakage, Iron Status After 6000m Ergometer Test in Elite Rowers.
Official title: Influance of 6000m Ergometer Test on Inflammatory, Permeability Markers and Iron Status.
Study type: OBSERVATIONAL
Overall status: COMPLETED
Conditions: Endothenial Dysfunction
Iron Status
Oxidative Stress
Keywords: endotoxin
gut permeability
gastrointestinal function
iron status
oxidative stress
Lead sponsor: Poznan University of Physical Education
Sponsor class: OTHER
First posted: 2025-03-24
Enrollment: 21
Sex: ALL
Minimum age (years): 18
Maximum age (years): 24
Standard ages: ADULT
Accepts healthy volunteers: yes
Observational model: COHORT
Time perspective: RETROSPECTIVE
Sampling method: NON_PROBABILITY_SAMPLE
Study population: Male rowers age 18 - 24 years.

Brief summary: The influence of a 6000m ergometer test on the markers of gut injury, endotoxemia, iron status, stress and inflammation in elite rowers.

Detailed description: The principal objective of our study was to assess gut permeability after completing a 6000-meter rowing test. The study was performed at the beginning of a competitive training phase. Eighteen elite Polish Rowing Team rowers participated in the study after applying the inclusion/exclusion criteria. The participants performed a 6000-meter ergometer test. Blood samples were taken before the test, after exercise, and after 1 hour of restitution. Parameters, such as I-FABP (intestinal fatty acid binding protein), LPS (lipopolysaccharide), LBP (lipopolysaccharide binding protein), claudin3, ferritin, transferrin, hepcidin, IL-6 (Interleukin-6), HO-1 (Heme Oxygenase 1), and ACSL4 (Long-chain-fatty-acid-CoA ligase 4 ) were determined using appropriate biochemical tests.

Eligibility criteria: Inclusion Criteria:

* minimum five years of training,
* five training sessions per week minimum,
* total training time minimum of 240 minutes,
* membership in the Polish Rowing Team,
* finishing the 6000-meter ergometer test.

Exclusion Criteria:

* antibiotic therapy within the last three months,
* probiotics and prebiotics within the last three months,
* dietary regime,
* gastrointestinal diseases.

Primary outcomes:
measure: I-FABP (intestinal fatty acid binding protein)to measure epithelial wall injury
timeFrame: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period
measure: LBP (lipopolysaccharide binding protein) to measure endotoxin
timeFrame: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period
measure: Claudin 3 to measure tight junction leakage
timeFrame: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period
measure: LPS (lipopolysaccharide) to measure endotoxin
timeFrame: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period
measure: Lactic acid to measure fatigue after the race
timeFrame: Blood samples was taken from earlobe at two-time points: before each stress test (after overnight fasting), one minute after the end of the test.
measure: Ferritin to measure iron status
timeFrame: Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period.
measure: Transferrin to measure iron status
timeFrame: Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period.
measure: Hepcidin to measure iron status
timeFrame: Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period.
measure: IL-6 (Interleukin-6) to measure inflammation
timeFrame: Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period.
measure: HO-1 (Heme Oxygenase 1) to measure oxidative stress
timeFrame: Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period.
measure: ACSL4 (Long-chain-fatty-acid-CoA ligase 4 ) to measure oxidative stress
timeFrame: Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period.
Secondary outcomes:
measure: energy
timeFrame: day before test, in the morning before the test
measure: protein
timeFrame: day before test, in the morning before the test
measure: carbohydrate
timeFrame: day before test, in the morning before the test
measure: fat
timeFrame: day before test, in the morning before the test
measure: fiber
timeFrame: day before test, in the morning before the test
measure: body mass
timeFrame: in the morning before the test
measure: Body composition - water
timeFrame: in the morning before the test
measure: Body composition - fat
timeFrame: in the morning before the test
measure: Lean body mass
timeFrame: in the morning before the test
measure: Height
timeFrame: in the morning before the test
measure: Gastrointestinal sypthomps self reported scale
timeFrame: up to 1 hours after the test
Other outcomes:
Locations:
city: Gorzów Wielkopolski
country: Poland
facility: Poznan University of Physical Education
state: Lubuskie
Study officials:
References: