Clinical trial NCT03712358: PVSRIPO for Patients With Unresectable Melanoma
Official title: A Phase I Trial of PVSRIPO for Patients With Unresectable Melanoma
Study type: INTERVENTIONAL
Overall status: TERMINATED
Phase: PHASE1
Conditions: Melanoma
Lead sponsor: Istari Oncology, Inc.
Sponsor class: INDUSTRY
Collaborators: name: Duke University
name: National Cancer Institute (NCI)
First posted: 2018-10-19
Enrollment: 12
Sex: ALL
Minimum age (years): 18
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: no
Allocation: NON_RANDOMIZED
Intervention model: SEQUENTIAL
Primary purpose: TREATMENT

Brief summary: This study is a Phase I study of oncolytic polio/rhinovirus recombinant (PVSRIPO) to primarily characterize the safety and tolerability of PVSRIPO in patients with AJCC Stage IIIB, IIIC, or IV melanoma in a modified 3+3 phase 1 trial design. Lesion biopsies and blood samples will be obtained pre- and post-injection throughout the study for routine histology/molecular genetic testing and immunologic analysis, respectively. Exploratory objectives include describing the response rates of PVSRIPO-injected versus non-injected lesion(s), the number of CD8 positive T cells present in the tumor biopsies before and after injection of PVSRIPO, and after PVSRIPO administration: the pathologic response in tumor biopsies, changes in the tumor microenvironment, and how systemic immune cell populations may change.

Detailed description: The Primary Objective of the study is to determine the safety profile of PVSRIPO in Stage IIIB, IIIC, and IV recurrent melanoma patients as determined by dose-limiting toxicities (DLT) by cohort, as well as in those retreated with PVSRIPO, when PVSRIPO is injected intralesionally into 1 to 3 or more cutaneous or subcutaneous lesions. As planned, up to 18 patients may be treated with PVSRIPO.

Biopsy material will be obtained from tumor tissue prior to and following virus administration, which may be subjected to routine histology along with molecular genetic testing and evaluation of pathological response. Whole blood for immunologic analyses will also be collected throughout the study period.

Routine study visits will occur through Day 126. Thereafter, visits will occur every 2-3 months for up to 2 years for subjects who do not progress. For patients with progressive disease, chart review only will occur every 3 months starting at the time of progression.

Patients who previously participated in Cohorts 0 through 3, who in the opinion of the investigator, may benefit from continued PVSRIPO administration, may be eligible to receive additional injections.

Eligibility criteria: Inclusion Criteria:

1. Undergone prior vaccination against PV and received a boost immunization with trivalent IPOL® (Sanofi-Pasteur SA) at least 1 week, but less than 6 weeks, prior to administration of PVSRIPO (within 6 months of PVSRIPO retreatment).

   a. Note: Patients who are unsure of their prior vaccination status/who have not been vaccinated must provide proof of vaccination and/or evidence of anti-PV immunity prior to enrollment, as applicable.
2. The patient must have received a boost immunization with trivalent inactivated IPOL™ (Sanofi-Pasteur) at least 1 week prior to administration of the study agent.
3. Patient must have histologically proven unresectable, recurrent, melanoma, stage IIIB, IIIC, or stage IV (AJCC staging must be documented in patient's medical record, as determined by CT of the chest, abdomen and pelvis, and/or whole body positron emission tomography (PET) scan, and MRI of the brain within 4 weeks prior to administration of study drug).
4. Patients with BRAF mutations, must have failed at least 2 lines of therapy, specifically one BRAF targeted therapy and at least one anti-PD-1 based therapy. For BRAF wild type, patients must have failed at least one anti-PD-1 based therapy.
5. Patient must be ≥ 18 years of age.
6. Patient must have an Eastern Cooperative Oncology Group (ECOG) / Zubrod status of 0-1.
7. Patient's disease must be bi-dimensionally measurable by caliper or radiological method as defined in the immune-related response criteria (irRC).
8. At least 1 injectable cutaneous, subcutaneous or nodal melanoma lesion ≥ 10 mm in longest diameter or, multiple injectable melanoma lesions which in aggregate have a longest diameter of ≥ 10 mm (Cohorts 0 and possibly 1). For cohorts where 2 or 3 injections are planned (Cohorts 1 and 2), the patient must have at least 2 injectable melanoma lesions (when 2 doses are planned) or ≥3 injectable melanoma lesions when at least 3 doses are planned in different lesions (Cohorts 2 through 4).

   a. Note: PVSRIPO retreatment requires ≥2 lesions amenable to injection.
9. At least one measurable lesion that will not be injected.
10. Serum lactate dehydrogenase (LDH) levels less than 1.5 x upper limit of normal (ULN).
11. Patient must have adequate bone marrow, liver and renal function as assessed by the following:

    1. Hemoglobin \> 9.0 g/dl
    2. White blood count (WBC) of \> 2000 m3
    3. Absolute neutrophil count (ANC) \> 1,000/mm3
    4. Platelet count \> 75,000/mm3
    5. Total bilirubin \< 2.0 x ULN
    6. Alanine aminotransferse (ALT) and aspartate aminotransferance (AST) \< 2.5 x the ULN

Exclusion Criteria:

1. Females who are pregnant or breast-feeding.
2. Adults of reproductive potential not employing an effective method of birth control.
3. Patients with severe, active co-morbidity, defined as follows:

   1. Patients with an active infection requiring treatment or having an unexplained febrile illness (Tmax \> 99.5°F/37.5°C).
   2. Patients with impaired cardiac function or clinically significant cardiac disease, such as congestive heart failure requiring treatment (New York Heart Association Class ≥ 2), uncontrolled hypertension or clinically significant arrhythmia; QTcF \> 470 msec on ECG if performed or congenital long QT syndrome; acute myocardial infarction or unstable angina pectoris \< 3 months prior to study.
   3. Patients with known lung (FEV1 \< 50%) disease or uncontrolled diabetes mellitus (HgbA1c\>7).
   4. Patients with albumin allergy.
   5. Autoimmune disease: History of or current active autoimmune diseases, \[e.g. including but not limited to inflammatory bowel diseases \[IBD\], rheumatoid arthritis, autoimmune thyroiditis, autoimmune hepatitis, systemic sclerosis (scleroderma and variants), systemic lupus erythematosus, autoimmune vasculitis, autoimmune neuropathies (such as Guillain-Barre syndrome)\]. Vitiligo and adequately controlled endocrine deficiencies such as hypothyroidism are not exclusionary.
   6. Known immunosuppressive disease, human immunodeficiency virus (HIV) infection, or chronic Hepatitis B or C.
4. Patients with a previous history of neurological complications due to PV infection.
5. Patients who have not recovered from the toxic effects of prior chemo- and/or radiation therapy. Guidelines for this recovery period are dependent upon the specific therapeutic agent being used. Toxicities must have resolved to CTCAE grade 1 or less with the following exceptions (alopecia, fatigue, vitiligo).
6. Patients with undetectable anti-tetanus toxoid IgG.
7. Patients with known history of agammaglobulinemia.
8. Patients on greater than 10 mg per day of prednisone within the 2 weeks prior to admission for PVSRIPO injection.
9. Patients with worsening steroid myopathy (history of gradual progression of bilateral proximal muscle weakness, and atrophy of proximal muscle groups).
10. Patients with prior, unrelated malignancy requiring current active treatment with the exception of cervical carcinoma in situ and adequately treated basal cell or squamous cell carcinoma of the skin.
11. Clinically active cerebral or bone metastases.
12. Greater than 3 visceral metastases (this does not include nodal metastases associated with visceral organs).
13. Prior allogeneic stem cell transplantation.
14. Concomitant therapy with any of the following: IL-2, interferon, or other non-study immunotherapy regimens; cytotoxic chemotherapy; immunosuppressive agents; other investigation therapies; or chronic use of systemic corticosteroids (used in the management of cancer or non-cancer-related illnesses). However, during the course of the study, use of corticosteroids is allowed if used for treating immune related adverse events (irAE), adrenal insufficiencies, or if administered at doses of prednisone 10 mg daily or equivalent.
15. Active clinically serious infection \> CTCAE Grade 2.
16. Antineoplastic therapy, radiotherapy, or any other investigational drug within 15 days prior to first study drug administration.

Primary outcomes:
measure: Proportion of Patients With Dose Limiting Toxicity by Cohort
timeFrame: 24 months
Secondary outcomes:
Other outcomes:
measure: Response Rates Via Measurement of Cutaneous Lesions Every 3 Weeks
timeFrame: 24 months
measure: Number of CD8 Positive T Cells by Immunohistochemistry on Pre Treatment and Post Treatment Biopsy (Cohorts 0-3 Only)
timeFrame: 4.1 months
measure: The Change in Tumor Pathology From Baseline to After PVSRIPO Injection
timeFrame: 4.1 months
measure: The Detection of Viral Replication in Injected Versus Non Injected Lesions (Cohorts 0-3 Only)
timeFrame: 4.1 months
measure: The Change in Inflammatory Cells and Markers After PVSRIPO in Injected Versus Non Injected Lesions (Cohorts 0-3 Only)
timeFrame: 4.1 months
measure: Change Relative to Baseline in Type and/or Function of T Cells Via Flow Cytometry (Cohorts 0-3 Only)
timeFrame: 24 months
Locations:
city: Durham
country: United States
facility: Duke University Medical Center
state: North Carolina
Study officials:
affiliation: Istari Oncology
name: Lisa Franklin
role: STUDY_DIRECTOR
References:
citation: Beasley GM, Brown MC, Farrow NE, Landa K, Al-Rohil RN, Selim MA, Therien AD, Jung SH, Gao J, Boczkowski D, Holl EK, Salama AKS, Bigner DD, Gromeier M, Nair SK. Multimodality analysis confers a prognostic benefit of a T-cell infiltrated tumor microenvironment and peripheral immune status in patients with melanoma. J Immunother Cancer. 2022 Sep;10(9):e005052. doi: 10.1136/jitc-2022-005052.
citation: Beasley GM, Nair SK, Farrow NE, Landa K, Selim MA, Wiggs CA, Jung SH, Bigner DD, True Kelly A, Gromeier M, Salama AK. Phase I trial of intratumoral PVSRIPO in patients with unresectable, treatment-refractory melanoma. J Immunother Cancer. 2021 Apr;9(4):e002203. doi: 10.1136/jitc-2020-002203.