Clinical trial NCT04748874: Immediate Versus Postponed Single Blastocyst Transfer in mNC-FET
Official title: Immediate Versus Postponed Single Blastocyst Transfer in Modified Natural Cycle Frozen Embryo Transfer (mNC-FET): a Multicenter Randomized Controlled Trial
Study type: INTERVENTIONAL
Overall status: ACTIVE_NOT_RECRUITING
Phase: NA
Conditions: Infertility
IVF
Keywords: Frozen embryo transfer
Immediate FET
Postponed FET
mNC FET
Lead sponsor: Rigshospitalet, Denmark
Sponsor class: OTHER
First posted: 2021-02-10
Enrollment: 464
Sex: FEMALE
Minimum age (years): 18
Maximum age (years): 40
Standard ages: ADULT
Accepts healthy volunteers: no
Allocation: RANDOMIZED
Intervention model: PARALLEL
Primary purpose: TREATMENT

Brief summary: The purpose of this randomized controlled trial is to investigate if immediate single blastocyst transfer (in the first menstrual cycle following oocyte retrieval) is non-inferior to standard postponed single blastocyst transfer (in the second or subsequent menstrual cycle following oocyte retrieval) in modified natural cycle frozen-thawed embryo transfer (mNC-FET) in terms of live birth rate.

Detailed description: The study is a multicenter randomized non-blinded controlled trial with the purpose of investigating if immediate mNC-FET is non-inferior to standard postponed mNC-FET in terms of live birth rate and other obstetric- and neonatal outcome.

Several fertility clinics in Denmark will participate in the recruitment of patients. All clinics perform standardized treatments according to the public health care system in Denmark. Patient enrolment is expected to begin in February 2021 and continue until December 2024.

The study population will consist of 464 patients undergoing mNC-FET after a fresh IVF/ICSI cycle that did not result in a viable pregnancy, or after a freeze-all cycle. Eligible patients will be recruited if they fulfil the inclusion criteria and none of the exclusion criteria. Patients will be randomized 1:1 by simple randomization to one of the following study arms:

I. Immediate mNC-FET In the Immediate arm, patients will undergo mNC-FET in the menstrual cycle immediately following oocyte retrieval with failed fresh embryo transfer or freeze-all.

II. Postponed mNC-FET In the Postponed arm, mNC-FET is performed at least one full menstrual cycle after the fresh embryo transfer or freeze-all cycle, which means that the first FET following the fresh cycle is not started until the second menstrual bleeding or later.

Patients who did not conceive after fresh embryo transfer, or patients undergoing a freeze-all cycle, will be invited to participate in the study if they have at least one vitrified blastocyst with embryo quality of minimum 3BB according to the Gardner score.

Patient related data and blood samples will be collected at baseline, day of ovulation trigger, day of blastocyst transfer and day of pregnancy testing for all participants. Patients enrolled at Rigshospitalet will have additional blood samples collected in the early- and mid-luteal phase. LH, FSH, progesterone and estradiol will be measured at all time-points. Blood used for these consecutive analyses will be destroyed immediately as a part of the daily laboratory routine. Additional blood samples will be collected and kept in a biobank at Rigshospitalet if patients agree. Patients are asked to sign a separate informed consent form for storage of blood samples in a biobank for future research projects.

Quality of life questionnaires will be collected at baseline and in the mid-luteal phase. If the woman has a partner, her or she will be asked to fill out quality of life questionnaires at the same time-points (requires separate informed consent).

Transvaginal ultrasound scans (TVUS) will be performed at baseline (cycle day 2-5) and on the day of ovulation trigger. Ovulation trigger by hCG (6500 IU) is administered when the dominant follicle reaches 17-18 mm. Single blastocyst warming and ultrasound guided transfer is performed 6 days after hCG trigger. If the patient conceives, a transvaginal scan will be performed at 7-8 weeks of gestation in order to assess viability and crown-rump length.

To compare ovarian morphology of the first cycle immediately following oocyte retrieval to the standard postponed cycle, a number of parameters, including ovarian volume and size and appearance of follicular structures \>10 mm, will be assessed with 2- and 3D TVUS at baseline and the day of hCG-trigger. 2D scans will be performed for all participants. 3D scans will be performed on a subgroup of participants at the same time points.

In case of pregnancy and delivery, data will be collected from the patient's medical records as well as the new-born child's birth records for registration of obstetric and neonatal outcomes. The custodians will be asked permission to access the upcoming child's journal for the purpose of gathering information about the birth and health of the child within the first year of life.

Data will be transferred to an online eCRF; REDCap. The REDCap database has a complete audit trail and is based on anonymous subject identification numbers used in the trial. Data are backed up daily and stored on a server located in a locked facility in Denmark.

The study is designed as a non-inferiority trial. A non-inferiority margin of 10% is considered clinically relevant. We expect a LBR of 25% per randomized study participant after postponed single blastocyst transfer in mNC-FET, which is considered the standard treatment. If there is truly no difference between the standard- and intervention treatment 464 participants (n=232 in each group) are required to be 80% sure that the upper limit of a one-sided 95% confidence interval (CI) (or equivalently a 90% two-sided CI) will exclude a difference in favour of the standard group of more than 10%.

As primary outcome, difference in LBR will be evaluated by means of risk difference with one-sided 95% CI. Non-inferiority will be concluded if the CI excludes a difference of more than 10% in favour of the standard treatment in intention-to-treat (ITT) and per protocol (PP) analyses. Difference in LBR per-transfer will be assessed as a secondary outcome by risk difference with 95% CI. Rate of positive hCG, ongoing pregnancy, miscarriage and cancelled cycles will be assessed by risk differences with 95% CI in PP, ITT and per-transfer analyses as outlined for LBR. Mean day of ovulation and mean levels of hormones will be compared with T-test. Hormone levels known to have a skewed distribution will be log-transformed prior to analysis. Number of ovarian follicular structures \>10 mm will be assessed with a chi-squared-test in a PP analysis. Time-to-pregnancy and live-birth per delivery will be compared in Kaplan-Meier plots and using log-rank test. Rates of pregnancy-related complications and adverse neonatal outcomes per delivery will be assessed using Fisher's exact test. Data on quality of life and psychosocial status will be obtained in a validated self-reported survey expressed by Likert based 5-scale items and compared by non-parametric Mann-Whitney U-tests. Statistical analyses will be performed using R.

Numbers and reasons for drop-out and cancellation will be tabulated for the two treatment groups and descriptive tables will be compiled for comparison of characteristics of drop-outs, cancelled cycles and completers within and between the groups. We anticipate a drop-out rate of at most 5% and a cancellation rate of at most 5%. In case of a differential or larger than expected drop-out or cancellation rate, potential biases will be discussed along with any discrepancies between the results of the PP, ITT and per-transfer analyses and conclusions will be drawn accordingly.

Eligibility criteria: Inclusion Criteria:

* Patients eligible for FET in a modified natural cycle
* Regular menstrual cycle (23-35 days)
* Vitrified day 5 or 6 blastocyst
* Blastocyst Gardner score \> or = 3BB at the day of vitrification

Exclusion Criteria:

* Uterine malformations or presence of hydrosalpinx
* Submucosal uterine myomas
* Uterine polyps
* Allergy to standard fertility medication
* Contradiction to standard fertility medication
* Male of female HIV, hepatitis B or C
* Preimplantation Genetic Testing (PGT) in the fresh cycle
* Severe OHSS during the fresh cycle (defined as need for ascites drainage and/or hospital admission)
* Oocyte donation
* Testicular sperm aspiration (TESA)

Primary outcomes:
measure: Live birth rate per randomized patient
timeFrame: One-year follow-up after a positive pregnancy test
measure: Live birth rate per protocol
timeFrame: One-year follow-up after a positive pregnancy test
Secondary outcomes:
measure: Live birth rate per blastocyst transfer
timeFrame: One-year follow-up after a positive pregnancy test
measure: Positive hCG rate per randomized patient and per blastocyst transfer
timeFrame: 16 days after ovulation trigger (hCG+16)
measure: Ongoing pregnancy rate per randomized patient and per blastocyst transfer
timeFrame: Ultrasound at 7-8 weeks of gestation
measure: Biochemical pregnancy loss
timeFrame: 16 days after ovulation trigger (hCG+16) and up to 7-8 weeks
measure: Clinical pregnancy loss
timeFrame: Routine ultrasound at 7-8 weeks of gestation or ad hoc ultrasound before 22 weeks of gestation
measure: Cycle cancellation
timeFrame: 16 days after ovulation trigger (hCG+16) and through study completion, up to 1 year
measure: Reason for cycle cancellation
timeFrame: 16 days after ovulation trigger (hCG+16) and through study completion, up to 1 year
measure: Number of ovarian follicular structures >10 mm
timeFrame: At baseline and on day of ovulation trigger (hCG+0), up to 1 month
measure: Day of ovulation
timeFrame: From first day of FET cycle to the day of ovulation trigger (hCG+0), up to 1 month
measure: Endocrinology of the luteal phase
timeFrame: Baseline, day of ovulation trigger (hCG+0), early luteal phase* (hCG+4), day of transfer (hCG+6) and mid-luteal phase* (hCG+11)(*only at Rigshospitalet), within one FET cycle, up to approximately 1 month
measure: Time-to-pregnancy
timeFrame: From day of ovarian stimulation to day of clinical pregnancy, up to 1 year
measure: Time-to-live-birth
timeFrame: From day of ovarian stimulation through study completion, up to 18 months
measure: Pregnancy related complications
timeFrame: One-year follow-up after a positive pregnancy test
measure: Neonatal outcome
timeFrame: One-year follow-up after a positive pregnancy test
measure: Quality of life assessment
timeFrame: Baseline and mid-luteal phase (hCG+11), up to 1 month
Other outcomes:
Locations:
city: Copenhagen
country: Denmark
facility: Fertility department, Rigshospitalet
Study officials:
affiliation: Fertility department , Rigshospitalet
name: Anja B Pinborg, Prof. DMSc
role: STUDY_DIRECTOR
References:
citation: Bergenheim SJ, Saupstad M, Pistoljevic N, Lyng Forman J, Larsen EC, Bogstad JW, Fynbo M, Hashem N, Freiesleben NC, Nohr B, Andersen LF, Humaidan P, Ziebe S, Pinborg AB, Lossl K. Immediate versus postponed single blastocyst transfer in modified natural cycle frozen embryo transfer (mNC-FET): a study protocol for a multicentre randomised controlled trial. BMJ Open. 2021 Oct 27;11(10):e053234. doi: 10.1136/bmjopen-2021-053234.