Clinical trial NCT05879341: Effect of Gene Polymorphism on Cognitive Function
Official title: The Effect of Irisin Polymorphism on Cognitive Function and Inter-individual Exercise Response Variability
Study type: OBSERVATIONAL
Overall status: UNKNOWN
Conditions: Polymorphosis
Cognitive Function
Keywords: Irisin
Cognition
Polymorphism
High intensity interval training
Lead sponsor: Kaohsiung Medical University Chung-Ho Memorial Hospital
Sponsor class: OTHER
Collaborators: name: Ministry of Science and Technology, Taiwan
First posted: 2023-05-30
Enrollment: 200
Sex: ALL
Minimum age (years): 20
Maximum age (years): 40
Standard ages: ADULT
Accepts healthy volunteers: yes
Observational model: ECOLOGIC_OR_COMMUNITY
Time perspective: CROSS_SECTIONAL
Sampling method: NON_PROBABILITY_SAMPLE
Study population: Taiwanese National who perform less than 120 minutes of aerobic exercise per week.

Brief summary: The goal of this observational study is to study the effect of gene polymorphism on cognitive improvement in response to exercise in healthy participants. The main questions it aims to answer are:

To investigate whether FNDC5/Irisin genotype polymorphism affects cognitive function and inter-individual variability in exercise response.

To explore whether the FNDC5/Irisin gene polymorphism can act alone or interact with the BDNF gene to cause inter-individual differences in cognitive function responses to exercise gain.

Participants will be asked to perform high-intensity interval training( HIIT) on a cycle ergometer. Each high-intensity training period will be 30 seconds followed by a 4-minute rest period.

The participants will also perform cognitive tests (Vistorian stroop test and Fitlight trainer test) before and after the exercise.

Detailed description: Exercise has a significant effect on improving brain function. It can improve cognitive function, enhance learning effects, and prevent the occurrence of neurodegenerative diseases in the elderly. Exercise's mechanism of action to improve cognitive function includes stimulating the nervous system to secrete brain-derived neurotrophic factor (BDNF) and skeletal muscle to secrete irisin. However, the effect of exercise on improving physical function varies from person to person. This phenomenon is called inter-individual exercise response variability. Genetic differences may be responsible for this phenomenon. The genotype differences of BDNF and Irisin can cause cognitive function. However, the scientific community is still unclear whether the differences between the two genotypes are related to individual differences in exercise responses. The investigators suspect that genotype might be responsible for interindividual variability in motor responses and therefore designed this experiment to test this thesis. This study is expected to recruit 200 healthy adults to complete the genotype sequencing of BDNF and Irisin and use the cognitive function responses before and after a single high-intensity interval aerobic exercise to establish the model that genotype affects the variability of individual exercise responses. The experimental results of this study can help the scientific community understand the influence of genotype on individual differences in exercise response and further explore and refine the design of exercise prescription.

Eligibility criteria: Inclusion Criteria:

* Taiwanese National who perform less than 120 minutes of aerobic exercise per week.

The subjects will refrain from drinking alcoholic or caffeinated drinks 24 hours prior to the participation in the study

Exclusion Criteria:

Subjects who are diagnosed with

* Hypertension
* Arrhythmia
* Depression
* Color blindness
* Musculoskeletal injury within one year

Body Mass index higher than 25

Pregnant females

Primary outcomes:
measure: Change in Serum BDNF levels
timeFrame: The blood sample will be collected immediately after the participant has signed the consent to assess pre exercise BDNF levels
measure: Change in Serum BDNF levels
timeFrame: The blood sample will be collected 30 minutes after the participant has completed high intensity exercise to assess post exercise BDNF levels
measure: Change in Serum Irisin Levels
timeFrame: The blood sample will be collected immediately after the participant has signed the consent to assess pre exercise Irisin levels
measure: Change in Serum Irisin Levels
timeFrame: The blood sample will be collected 30 minutes after the participant has completed high intensity exercise to assess post exercise Irisin levels
measure: Change in Stroop Reaction time
timeFrame: Baseline
measure: Change in Stroop Reaction time
timeFrame: Immediately after the procedure
measure: Change in Fitlight reaction time
timeFrame: Baseline
measure: Change in Fitlight reaction time
timeFrame: Immediately after the procedure
Secondary outcomes:
measure: Monitor the Heart rate
timeFrame: During the procedure
measure: Change in Fitlight simple reaction time
timeFrame: Baseline
measure: Change in Fitlight simple reaction time
timeFrame: Immediately after the procedure
Other outcomes:
Locations:
city: Kaohsiung
country: Taiwan
facility: Kaohsiung Medical University
Study officials:
affiliation: Kaohsiung Medical University
name: Dean-Chuan Wang, PhD
role: PRINCIPAL_INVESTIGATOR
References: