Clinical trial NCT04679519: The Effects of Protein Supplementation in Females and Males Following Acute Eccentric Exercise
Official title: The Effects of Protein Supplementation in Females and Males Following Acute Eccentric Exercise
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Muscle Damage
Lead sponsor: Liverpool John Moores University
Sponsor class: OTHER
First posted: 2020-12-22
Enrollment: 90
Sex: ALL
Minimum age (years): 18
Maximum age (years): 35
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: PARALLEL
Intervention model description: Randomised control study with four groups, one control and three protein supplements.
Primary purpose: TREATMENT

Brief summary: Examine the impact of the protein supplements leucine, HMB and BCAA on muscle recovery and inflammatory response following acute exercise.

Detailed description: Participants, both females and males, will perform an acute bout of exercise. A bout of exercise that will involve multiple drop jumps. Prior to and post exercise, functional outputs will be measured and blood will be collected to quantify the inflammatory response. The protocol will involve attending the laboratory on 6 occasions over a two week period to complete the above measures.

Eligibility criteria: Inclusion Criteria:

* Health recreationally active males and females.

Exclusion Criteria:

* Asthma sufferers
* People with kidney disease
* People partaking in lower limb strength training
* People suffering DOMS in the past 6 months
* People already taking dietary supplements, other NSAIDs, or for example aspirin, alcohol, warfarin, phenytoin, methotrexate, lithium, diuretics, cyclosporine.
* People with bone or soft tissue injury within last 12 months
* People with gastric problems
* People with hypertension
* People who have had history of heart failure
* People with liver disease
* People with connective tissue disorders
* People with Crohn's disease
* People with ulcerative colitis
* People with peripheral arterial disease
* People with cerebrovascular disease
* Elderly population
* People with allergies to NSAIDs, Omega- 3 fatty acids and BCAAs.
* People with depression
* People with bipolar disorder
* People with diabetes
* Females who are pregnant
* People who have HIV / AIDS

Primary outcomes:
measure: Concentration of Creatine Kinase at baseline
timeFrame: Baseline
measure: Concentration of Creatine Kinase immediately after intervention.
timeFrame: Immediately after intervention.
measure: Concentration of Creatine Kinase at 24 hours after intervention.
timeFrame: 24 hours after intervention.
measure: Concentration of Creatine Kinase at 48 hours after intervention.
timeFrame: 48 hours after intervention.
measure: Concentration of Creatine Kinase at 7 days after intervention.
timeFrame: 7 days after intervention.
measure: Concentration of Creatine Kinase at 14 days after intervention.
timeFrame: 14 days after intervention.
measure: Number of Chair Rises at baseline
timeFrame: Baseline
measure: Number of Chair Rises immediately after intervention.
timeFrame: Immediately after intervention.
measure: Number of Chair Rises at 24 hours after intervention.
timeFrame: 24 hours after intervention.
measure: Number of Chair Rises at 48 hours after intervention.
timeFrame: 48 hours after intervention.
measure: Number of Chair Rises at 7 days after intervention.
timeFrame: 7 days after intervention.
measure: Number of Chair Rises at 14 days after intervention.
timeFrame: 14 days after intervention.
measure: Squat Jump height at baseline
timeFrame: Baseline
measure: Squat Jump height immediately after intervention.
timeFrame: Immediately after intervention.
measure: Squat Jump height at 24 hours after intervention.
timeFrame: 24 hours after intervention.
measure: Squat Jump height at 48 hours after intervention.
timeFrame: 48 hours after intervention.
measure: Squat Jump height at 7 days after intervention.
timeFrame: 7 days after intervention.
measure: Squat Jump height at 14 days after intervention.
timeFrame: 14 days after intervention.
measure: Range of motion at baseline.
timeFrame: Baseline
measure: Range of motion immediately after intervention.
timeFrame: Immediately after intervention.
measure: Range of motion at 24 hours after intervention.
timeFrame: 24 hours after intervention.
measure: Range of motion at 48 hours after intervention.
timeFrame: 48 hours after intervention.
measure: Range of motion at 7 days after intervention.
timeFrame: 7 days after intervention.
measure: Range of motion at 14 days after intervention.
timeFrame: 14 days after intervention.
measure: Inflammatory cytokines concentration at baseline
timeFrame: Baseline
measure: Inflammatory cytokines concentration at 24 hours after intervention.
timeFrame: 24 hours after intervention.
measure: Inflammatory cytokines concentration at 48 hours after intervention.
timeFrame: 48 hours after intervention.
measure: Inflammatory cytokines concentration at 7 days after intervention.
timeFrame: 7 days after intervention.
measure: Concentration of Lactate from baseline to immediately after intervention.
timeFrame: Baseline and immediately after intervention.
measure: Muscle Soreness
timeFrame: Daily for 14 days.
Secondary outcomes:
measure: Weighed food diaries.
timeFrame: Daily for 14 days.
Other outcomes:
Locations:
city: Liverpool
country: United Kingdom
facility: Liverpool John Moores University
state: Merseyside
Study officials:
References:
citation: Howatson G, Hoad M, Goodall S, Tallent J, Bell PG, French DN. Exercise-induced muscle damage is reduced in resistance-trained males by branched chain amino acids: a randomized, double-blind, placebo controlled study. J Int Soc Sports Nutr. 2012 Jul 12;9:20. doi: 10.1186/1550-2783-9-20. eCollection 2012.
citation: Kirby TJ, Triplett NT, Haines TL, Skinner JW, Fairbrother KR, McBride JM. Effect of leucine supplementation on indices of muscle damage following drop jumps and resistance exercise. Amino Acids. 2012 May;42(5):1987-96. doi: 10.1007/s00726-011-0928-9. Epub 2011 May 12.