Clinical trial NCT05111717: The Effect of Desfluran On Global Dna Methylation
Official title: The Effect of Desfluran Administered in General Anesthesia On Global Dna Methylation
Study type: OBSERVATIONAL
Overall status: COMPLETED
Conditions: DNA Damage
Keywords: Desflurane,
DNA methylation
epigenetics
inhalation anesthetics
Lead sponsor: Muğla Sıtkı Koçman University
Sponsor class: OTHER
First posted: 2021-11-08
Enrollment: 40
Sex: ALL
Minimum age (years): 18
Maximum age (years): 65
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: no
Observational model: OTHER
Time perspective: PROSPECTIVE
Sampling method: PROBABILITY_SAMPLE
Study population: Patients with physical status I and II of the American Society of Anesthesiologists (ASA), aged between 18-65 and scheduled for lumbar spinal surgery, which will take at least 120 minutes, will be included in the study

Brief summary: The purpose of this study is to demonstrate the molecular effect of desflurane on DNA by measuring the global DNA methylation level in patients under going lumbar spinal stenosis surgery under general anesthesia.

Detailed description: Forty patients scheduled for lumbar spinal surgery will include in the study. Peripheral venous blood samples will collect prior to the induction of anesthesia (T1) and after 120 minutes (T2). Anesthesia will maintaine with 40%-60% O2-air mixture and 6% desflurane. DNA will obtain from blood samples with the help of DNA isolation kit and global DNA methylation analysis will perform by ELISA method.

Eligibility criteria: Inclusion Criteria:

1. Ability to understand and willingness to sign an informed consent form
2. Elective lumbar spinal surgery that will take at least 120 minutes,
3. American Society of Anesthesiologists (ASA) physical status I and II
4. Be between the ages of 18 and 65

Exclusion Criteria:

1. Patients with ASA physical status III or IV disease
2. Smoking or alcohol consumption
3. Chronic systemic disease (diabetes mellitus,chronic pulmonary disorders, chronic kidney and liver disorders
4. recently received radiation, chemotherapy
5. malignancy
6. blood transfusion in operation
7. use of antioxidant supplements
8. have received general anesthesia in the last three months
9. Patients with known occupational exposures (operating room personnel, chemical plant workers

Primary outcomes:
measure: Global DNA methylation change 5 minutes before and 120 minutes after anesthesia induction in peripheral blood mononuclear cells
timeFrame: 5 minutes before induction of anesthesia ,120 minutes after the beginning of anesthesia
Secondary outcomes:
Other outcomes:
Locations:
city: Mugla
country: Turkey
facility: Mugla Sıtkı Kocman University Training and research Hospital
Study officials:
References:
citation: Chowdhury B, Cho IH, Irudayaraj J. Technical advances in global DNA methylation analysis in human cancers. J Biol Eng. 2017 Mar 1;11:10. doi: 10.1186/s13036-017-0052-9. eCollection 2017.
citation: Jaloszynski P, Kujawski M, Wasowicz M, Szulc R, Szyfter K. Genotoxicity of inhalation anesthetics halothane and isoflurane in human lymphocytes studied in vitro using the comet assay. Mutat Res. 1999 Feb 19;439(2):199-206. doi: 10.1016/s1383-5718(98)00195-8.
citation: Bilban M, Jakopin CB, Ogrinc D. Cytogenetic tests performed on operating room personnel (the use of anaesthetic gases). Int Arch Occup Environ Health. 2005 Feb;78(1):60-4. doi: 10.1007/s00420-004-0579-1. Epub 2005 Feb 10.