Clinical trial NCT06909799: A Confirmatory Trial of Adjunctive NAC to Prevent Post Tuberculosis Lung Disease
Official title: A Confirmatory Trial of Adjunctive N-acetylcysteine to Prevent Post Tuberculosis Lung Disease (NAC-PTLD) - A TB SEQUEL II Substudy
Study type: INTERVENTIONAL
Overall status: RECRUITING
Phase: NA
Conditions: Tuberculosis (TB)
Keywords: NAC
N-acetylcysteine
HDT
Host directed therapy
PTLD
Post TB lung disease
Lead sponsor: The Aurum Institute NPC
Sponsor class: OTHER
Collaborators: name: Ludwig-Maximilians - University of Munich
name: Research Center Borstel
First posted: 2025-04-04
Enrollment: 242
Sex: ALL
Minimum age (years): 18
Maximum age (years): 65
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: no
Allocation: RANDOMIZED
Intervention model: PARALLEL
Intervention model description: NAC dosing will be 1800mg PO BID during months 1-6, or control, depending on study arm. All participants will also receive standard TB treatment.
Primary purpose: PREVENTION

Brief summary: The goal of this clinical trial is to evaluate if N-acetylcysteine (NAC) works to prevent post-tuberculosis lung disease (PTLD) in patients with severe pulmonary impairment. It also aims to assess the safety of NAC. The main questions the study aims to answer are: Does NAC improve lung function (FEV1%) over 12 months in participants with pulmonary tuberculosis and baseline risk factors for PTLD? What medical issues or adverse events do participants experience while taking NAC? Researchers will compare NAC treatment to a control group to see if it can prevent PTLD when given in addition to standard TB treatment.

Participants will: Take NAC (1800mg twice daily) for 6 months with standard TB treatment or receive standard TB treatment alone; Attend scheduled clinic visits for 12 months, during which they will have respiratory assessments, blood tests, and symptom monitoring; Complete quality-of-life questionnaires and provide sputum and blood samples for analysis at multiple time points.

Detailed description: Study Summary: This prospective, randomized, controlled, parallel-arm, open-label clinical trial evaluates the efficacy of N-acetylcysteine (NAC) as an adjunctive therapy for persons with pulmonary tuberculosis and risk factors for PTLD. The study aims to determine the long-term impact of NAC on lung function, respiratory symptoms, and quality of life in patients with drug-sensitive, culture-confirmed pulmonary tuberculosis (TB).

Study Rationale: Pulmonary TB is a leading cause of chronic lung impairment globally. PTLD results in significant morbidity even after successful TB treatment. Prior research suggests NAC may mitigate oxidative stress and preserve lung function in TB patients. This trial seeks to confirm and expand findings from the NAC-TB sub-study of TB Sequel regarding PTLD prevention and treatment.

This trial aims to provide robust evidence on the role of NAC in PTLD management, potentially informing future TB treatment guidelines.

Eligibility criteria: Inclusion Criteria:

1. Persons aged 18 to 65 years
2. Willing and able to provide signed written consent, or witnessed oral con-sent with thumbprint in the case of illiteracy, prior to undertaking any trial-related procedures.
3. Body weight (in light clothing without shoes) between 30 and 90 kg.
4. Xpert TB/RIF OR Ultra showing RIF-S-MTB, with subsequent confirmation of MTB by culture.
5. Chest radiograph meeting criteria for moderate or far advanced pulmonary tuberculosis 1
6. FEV1 ≤65% of predicted adjusted for age, height, sex, and race
7. If female, of child-bearing potential and sexually active, willing to use a contraceptive method for the duration of study participation
8. HIV-1/2 seronegative, or if seropositive: CD4 T cell count ≥100/mcL and either currently receiving ART or willing to start ART during study participation

Exclusion Criteria:

1. Any condition for which participation in the trial, as judged by the investigator, could compromise the well-being of the subject or prevent, limit or confound protocol specified assessments, such as pneumothorax or clinically significant pleural effusion
2. Pregnancy or breast-feeding
3. Is critically ill, and in the judgment of the investigator has a diagnosis likely to result in death during the trial or the follow-up period.
4. TB meningitis or other forms of severe tuberculosis with high risk of a poor outcome as judged by the investigator.
5. History of allergy or hypersensitivity to any of the trial therapies or related substances, including known allergy or suspected hypersensitivity to rifampin.
6. Having participated in other clinical trials with investigational agents within 8 weeks prior to trial start or currently enrolled in an investigational trial.
7. No more than 5 days treatment for the current TB episode, and no other TB treatment in the preceding 6 months
8. Angina pectoris requiring treatment with nitroglycerin or other nitrates
9. Cardiac arrhythmia requiring medication, or any clinically significant ECG abnormality, in the opinion of the investigator
10. Random blood glucose \>140 mg/dL (or \>7.8 mmol/L), or history of unstable Diabetes Mellitus which required hospitalization for hyper- or hypoglycaemia within the past year prior to start of screening.
11. Use of systemic corticosteroids within the past 28 days, or a likely to require corticosteroids for management of another medical condition during the period of study participation.
12. Patients requiring treatment with medications not compatible with rifampin, such as HIV protease inhibitors
13. Subjects with any of the following abnormal laboratory values:

    1. creatinine \>2 mg/dL
    2. haemoglobin \<8 g/dL
    3. platelets \<100x109 cells/L
    4. serum potassium \<3.5
    5. aspartate aminotransferase (AST) ≥2.0 x ULN
    6. alkaline phosphatase (AP) \>5.0 x ULN
    7. total bilirubin \>1.5 mg/dL
    8. positive HBsAg

Primary outcomes:
measure: FEV1% of predicted at month 12
timeFrame: month 12
Secondary outcomes:
measure: FEV1%
timeFrame: Through study completion, an average of 12 months
measure: FVC%
timeFrame: Through study completion, an average of 12 months
measure: FEV/FVC
timeFrame: Through study completion, an average of 12 months
measure: Exacerbations
timeFrame: Through study completion, an average of 12 months
measure: Respiratory QoL at multiple time points
timeFrame: Through study completion, an average of 12 months
measure: Respiratory symptoms
timeFrame: Through study completion, an average of 12 months
measure: Whole blood total glutathione
timeFrame: Through study completion, an average of 12 months
measure: Mtb sputum culture
timeFrame: Month 2, 3 and 6
measure: Change in MGIT (Mycobacterial Growth Indicator Tube) time to positivity (TTP): assessed over time through repeated measurements
timeFrame: Through study completion, an average of 12 months
measure: Treatment failure
timeFrame: Through study completion, an average of 12 months
measure: TB recurrence
timeFrame: Through study completion, an average of 12 months
measure: Number and severity of hepatic safety events
timeFrame: Through study completion, an average of 12 months
measure: Number and severity of adverse events (SAEs)
timeFrame: Through study completion, an average of 12 months
Other outcomes:
Locations:
city: Fajara
country: Gambia
facility: MRC Unit The Gambia at LSHTM
state: The Gambia
Study officials:
affiliation: Aurum Institute
name: Robert Wallis - Professor
role: PRINCIPAL_INVESTIGATOR
affiliation: LMU
name: Andrea Rachow, MD
role: PRINCIPAL_INVESTIGATOR
References:
citation: Wallis RS, Sabi I, Lalashowi J, Bakuli A, Mapamba D, Olomi W, Siyame E, Ngaraguza B, Chimbe O, Charalambous S, Rachow A, Ivanova O, Zurba L, Myombe B, Kunambi R, Hoelscher M, Ntinginya N, Churchyard G. Adjunctive N-Acetylcysteine and Lung Function in Pulmonary Tuberculosis. NEJM Evid. 2024 Sep;3(9):EVIDoa2300332. doi: 10.1056/EVIDoa2300332. Epub 2024 Aug 27.