Clinical trial NCT03361618: Effect of Oral Zinc Sulfate Supplementation on Enzymes of Urate Pathway
Official title: Effect of Oral Zinc Sulfate Supplementation on Enzymes of Urate Pathway in Spermatozoa and Seminal Plasma of Iraqi Asthenospermic Patients
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Infertility Unexplained
Keywords: Uric acid
Adenosine deaminase
Zinc Sulfate
5'-nucleotidase
Asthenospermia.
Xanthine oxidase
Lead sponsor: Babylon University
Sponsor class: OTHER
Collaborators: name: Ministry of Health, Iraq
First posted: 2017-12-05
Enrollment: 120
Sex: MALE
Minimum age (years): 26
Maximum age (years): 36
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: NON_RANDOMIZED
Intervention model: PARALLEL
Intervention model description: Semen volume after treatment.
Primary purpose: TREATMENT

Brief summary: Although many reports have investigated the relationship between subfertility and uric acid levels in semen, to the best of author knowledge, there is no study have been published to yet focus on the effects of asthenospermia treatments, such as given an oral zinc supplementation, on the activity of urate-related enzymes, which are important in fertility of human.

Detailed description: The present study was designed to investigate the effect of zinc supplementation on the qualitative and quantitative characteristics of semen, along with uric acid levels and urate-related enzymes in the seminal plasma and spermatozoa of asthenospermic males.

Eligibility criteria: Inclusion Criteria:

* The presence of asthenozoospermia in the semen sample.
* Must be unable to reproduce.

Exclusion Criteria:

* the absence of endocrinopathy, varicocele, and female factor infertility. Smokers and alcoholic men were excluded from the study because of their recognized high seminal ROS levels and decreased antioxidant levels.

Primary outcomes:
measure: Measurement the uric acid level in spermatozoa and seminal plasma by using spectrophotometer.
timeFrame: up to three months.
Secondary outcomes:
measure: Measurement the adenosine deaminase activity in spermatozoa and seminal plasma by using spectrophotometer.
timeFrame: up to three months.
measure: Measurement the xanthine oxidase activity in spermatozoa and seminal plasma by using spectrophotometer.
timeFrame: up to three months.
measure: Measurement the 5'-nucleotidase activity in spermatozoa and seminal plasmaby using the spectrophotometer.
timeFrame: up to three months.
Other outcomes:
Locations:
city: Hilla
country: Iraq
facility: Mahmoud Hussein Hadwan
state: Babylon
Study officials:
affiliation: University of Babyblon
name: Abdul Razzaq A. Alsalman, PhD
role: STUDY_CHAIR
affiliation: University of Babyblon
name: Lamia A. Almashhedy, PhD
role: STUDY_DIRECTOR
affiliation: University of Babyblon
name: Mahmoud Hussein Hadwan, PhD
role: PRINCIPAL_INVESTIGATOR
affiliation: University of Babyblon
name: Abdulsamie H Alta'ee, PhD
role: STUDY_DIRECTOR
References:
citation: Alsalman ARS, Almashhedy LA, Alta'ee AH, Hadwan MH. Effect of Zinc Supplementation on Urate Pathway Enzymes in Spermatozoa and Seminal Plasma of Iraqi Asthenozoospermic Patients: A Randomized Controlled Trial. Int J Fertil Steril. 2020 Jan;13(4):315-323. doi: 10.22074/ijfs.2020.5760. Epub 2019 Nov 11.