Clinical trial NCT05521919: Acute Plasma Abeta Responses to Stress
Official title: Effects of Acute Stress Exposure on Plasma Beta-amyloid Levels
Study type: INTERVENTIONAL
Overall status: RECRUITING
Phase: NA
Conditions: Stress Reaction
Stress Physiology
Keywords: Stress Reaction
Stress
Amyloid-Beta
Lead sponsor: University of Southern California
Sponsor class: OTHER
Collaborators: name: Washington University School of Medicine
name: Keck School of Medicine of USC
First posted: 2022-08-30
Enrollment: 38
Sex: ALL
Minimum age (years): 18
Maximum age (years): 65
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: CROSSOVER
Intervention model description: Participants will complete one session of each condition: stress and no-stress. Participants will be randomly assigned to one of the two conditions for their first session. Participants will then be randomly assigned to the remaining condition for the second session.
Primary purpose: BASIC_SCIENCE

Brief summary: Research has shown that activation of the sympathetic nervous system for extended periods or during certain times in life can increase the risk for Alzheimer's disease. Some research in animal models show that acute activation of the sympathetic nervous system through stress exposure can increase certain Alzheimer's disease-related biomarkers, such as amyloid-beta, within hours of exposure. However, how acute sympathetic nervous system activation via stress exposure affects amyloid-beta levels in humans has yet to be examined.

In this study, the investigators will examine whether brief increases in sympathetic activation result in immediate changes in plasma amyloid-beta levels in the five hours after exposure.

Detailed description: Aim 1: determine whether acute exposure to stress (increasing sympathetic nervous system activation) increases plasma amyloid-beta-40 and amyloid-beta-42 in the five hours following exposure.

Eligibility criteria: Inclusion Criteria:

* Healthy adult weighing at least 110 pounds
* No chronic conditions or illness
* Taking no medications or drugs that might alter the stress response (e.g., corticosteroids, beta-blockers, and others)
* Not using any hormone-containing medication (e.g., hormonal contraceptives, hormone replacement, etc.)
* No contraindications for cold pressor test exposure (high blood pressure, heart disease, dysrhythmia, peripheral vascular disease, any other cardiovascular disorder, diabetes, vasculitis, lupus, tingling or numbness in the hands and/or feet, cryoglobulinemia, and Reynaud's phenomenon)
* No history of fainting during blood draws
* No phobia of having their blood drawn
* No general history of fainting or seizures
* Not currently diagnosed with a mood disorder (e.g., major depressive disorder)
* Not pregnant in the past 12 months
* Not lactating in the past 12 months
* Not trying to become pregnant if premenopausal
* Experienced natural menopause if postmenopausal
* Be non-smokers
* Be fluent in English

Exclusion Criteria:

- Unwilling to be filmed during the stress task OR unwilling to sign the waiver allowing their filmed responses to be used in scientific presentations

Primary outcomes:
measure: Plasma amyloid-beta-40
timeFrame: 9.5 hours
measure: Plasma amyloid-beta-42
timeFrame: 9.5 hours
Secondary outcomes:
Other outcomes:
Locations:
city: Los Angeles
country: United States
facility: University of Southern California
state: California
Study officials:
References: