Clinical trial NCT05574413: Impact of Acute Exercise Intensity and Pattern on Cytokine Function
Official title: Impact of Acute Exercise Intensity and Pattern on Cytokine Function
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Inflammation
Keywords: Exercise
Cytokines
Leukocytes
Interleukin-10
Interleukin-6
Lead sponsor: University of British Columbia
Sponsor class: OTHER
First posted: 2022-10-10
Enrollment: 16
Sex: ALL
Minimum age (years): 18
Maximum age (years): 35
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: CROSSOVER
Intervention model description: Repeated measures randomized counterbalanced crossover design
Primary purpose: BASIC_SCIENCE

Brief summary: The immune system helps prevent illness, fights off infections, and repairs damaged tissues following an injury. However, when immune cells remain active for prolonged periods of time - a state known as "chronic inflammation" - they can contribute to the development and progression of chronic diseases like heart disease and diabetes. Exercise can reduce the risk of developing many of these diseases and at least part of the health benefits of exercise are due to the ability of exercise to reduce "chronic inflammation". The inflammation-lowering effects of exercise are typically captured by measuring hormone-like molecules released from immune cells called "cytokines" in the blood. In addition to changes in circulating cytokine levels, exercise may also alter how immune cells respond to these cytokines. How exercise intensity (i.e., how hard you are working during exercise) and pattern (i.e., exercising as a long continuous bout or in short intervals) impact the ability of immune cells to respond to cytokines is not well understood. A better understanding of how exercise intensity and pattern of exercise for reducing chronic inflammation may help determine the best types of exercises for improving health and preventing chronic diseases.


Eligibility criteria: Inclusion Criteria:

* 18-35 years of age
* Body mass index between 18.5-30 kg/m\^2
* Free of cardiometabolic and autoimmune/inflammatory disease

Exclusion Criteria:

* Competitive endurance athlete
* Cigarette smoker
* Currently taking immunomodulatory/anti-inflammatory medications
* Currently pregnant

Primary outcomes:
measure: IL-10 mediated STAT3 phosphorylation
timeFrame: Change from pre-exercise to immediately and 90-min post-exercise
Secondary outcomes:
measure: IL-10 mediated TNF-alpha inhibition
timeFrame: Change from pre-exercise to immediately and 90-min post-exercise
measure: IL-6 mediated STAT3 phosphorylation
timeFrame: Change from pre-exercise to immediately and 90-min post-exercise
measure: IL-6 mediated TNF-alpha inhibition
timeFrame: Change from pre-exercise to immediately and 90-min post-exercise
measure: Plasma IL-10
timeFrame: Change from pre-exercise to immediately, 30-, and 90-min post-exercise
measure: Plasma IL-6
timeFrame: Change from pre-exercise to immediately, 30-, and 90-min post-exercise
measure: Plasma TNF-alpha
timeFrame: Change from pre-exercise to immediately, 30-, and 90-min post-exercise
measure: Hematology panel
timeFrame: Change from pre-exercise to immediately, 30-, and 90-min post-exercise
measure: Extracellular vesicles
timeFrame: Change from pre-exercise to immediately, 30-, and 90-min post-exercise
Other outcomes:
Locations:
city: Kelowna
country: Canada
facility: UBC Okanagan
state: British Columbia
Study officials:
affiliation: UBC Okanagan
name: Jonathan P Little, PhD
role: PRINCIPAL_INVESTIGATOR
References: