Clinical trial NCT02816645: One-year Follow-up of Iron in Basal Ganglia - R2*: a Biomarker of Parkinson's Disease Progression?
Official title: One-year Follow-up of Iron in Basal Ganglia - R2*: a Biomarker of Parkinson's Disease Progression?
Study type: INTERVENTIONAL
Overall status: UNKNOWN
Phase: NA
Conditions: Parkinson's Disease
Keywords: Parkinson's Disease
Biomarker
Iron
R2*
MRI
Patients
UK Parkinson's Disease Society Brain Bank
Lead sponsor: University Hospital, Clermont-Ferrand
Sponsor class: OTHER
Collaborators: name: France Parkinson Association
name: Federation for Brain Research
name: NS-PARK Network
First posted: 2016-06-28
Enrollment: 160
Sex: ALL
Minimum age (years): 40
Maximum age (years): 80
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: yes
Allocation: NON_RANDOMIZED
Intervention model: PARALLEL
Primary purpose: DIAGNOSTIC

Brief summary: The study of non-invasive and reliable biomarkers to track progression of Parkinson's disease (PD) is essential while disease-modifying treatments are being developed. Many clinical biological or imaging biomarkers have been tested but no "gold standard" has been found as of yet. Among these, Magnetic Resonance Imaging (MRI) relaxometry using R2\* measurement (R2\* = 1/T2\*), which is a validated marker for estimating brain iron concentration, appears to be an attractive technique because its safety, rapidly measured in clinical conditions and its ease to ensure individual longitudinal follow-up. Current data of cross sectional studies of R2\*, which have shown an iron increase in Substantia Nigra (SN), led to suppose that it could be a biomarker of disease vulnerability. Recently, the investigators have conducted the first longitudinal follow-up of R2\* (1.5 T MRI), which showed a rapid R2\* increase in both parts of the SN and in the caudal putamen. We propose, here, a multicenter prospective study of one-year cohort follow-up of R2\* variations (ΔR2\*) in three regions of interest (ROIs) (the SN, the Ventral Tegmental Area (VTA) and the Putamen) of 160 patients with PD, using a 3 Tesla MRI, to evaluate the potential interest of R2\* as a biomarker of disease progression. The variation of R2\* (ΔR2\*) will be correlated with clinical markers of disease progress, non-motor symptoms. 80 healthy controls subjects will also be included to assess the effect of aging on cerebral physiological iron levels.

Detailed description: Use lay language.

The study of non-invasive and reliable biomarkers to track progression of Parkinson's disease is essential while disease-modifying treatments are being developed. Many clinical biological or imaging biomarkers have been tested but no "gold standard" has been found as of yet. Among these, Magnetic Resonance Imaging (MRI) relaxometry using R2\* measurement (R2\* = 1/T2\*), which is a validated marker for estimating brain iron concentration, appears to be an attractive technique because its safety, rapidly measured in clinical conditions and its ease to ensure individual longitudinal follow-up. Current data of cross sectional studies of R2\*, which have shown an iron increase in substantia nigra, led to suppose that it could be a biomarker of disease vulnerability. Recently, we have conducted the first longitudinal follow-up of R2\* (1.5 T MRI), which showed a rapid R2\* increase in both parts of the SN and in the caudal putamen. We propose, here, a multicenter prospective study of one-year cohort follow-up of R2\* variations (ΔR2\*) in three regions of interest (the substantia nigra, the ventral tegmental area and the putamen) of 160 patients with Parkinson's disease, using a 3 Tesla MRI, to evaluate the potential interest of R2\* as a biomarker of disease progression. The variation of R2\* (ΔR2\*) will be correlated with clinical markers of disease progress, non-motor symptoms. 80 healthy controls subjects will also be included to assess the effect of aging on cerebral physiological iron levels.

Type of study : Interventional multicenter prospective study of cohort follow-up.

Number of centers : 6 (Clermont-Ferrand, Lyon, Grenoble, Paris, Limoges, Lille)

Study population :

Recruitment

160 patients with Parkinson's disease divided into four subgroups of 40 patients according to disease duration:

* \< 5 years
* Between 5 and 10 years
* Between 10 and 15 years
* \> 15 years

In parallel, 80 sex-age matched healthy controls subjects matched equally distributed in the 4 groups (n = 20/group) based on a stratified plan by gender and age (ratio 1:2).

Subjects will be assessed twice, one year apart by the procedures detailed below. The two neurological assessments should be made by the same certified neurologist.

Patients' procedure:

Visit 1 (Day 0) (duration: 1 day or 2 half days)

* Signature of an informed consent form (only at Day 0).
* Demographic and clinical characteristics (sex, age, disease duration, treatments).
* Neurological evaluation.
* Neuropsychological evaluation.
* Self-administered questionnaires.
* 1st MRI acquisition.

Visit 2 (Day 0 + 1 year) (duration: 1 day or 2 half days)

* Current treatment(s), adverse event(s), serious adverse event(s).
* Neurological evaluation.
* Neuropsychological evaluation.
* Self-administered questionnaires.
* 2d MRI acquisition.

Matched healthy controls subjects' procedure:

Visit 1 (Day 0) (duration: 1 half day)

* Signature of an informed consent form (only at Day 0).
* Demographic and clinical characteristics (sex, age, disease duration, treatments).
* Brief neuropsychological evaluation.
* 1st MRI acquisition.

Visit 2 (Day 0 + 1 year) (duration: 1 half day)

* Current treatment(s), adverse event(s), serious adverse event(s).
* Brief neuropsychological evaluation.
* 2d MRI acquisition.

MRI acquisition (duration: 45 to 60 min)

The procedure will be performed on a 3 Tesla MRI, allowing a substantial gain in signal-to-noise ratio compared with the one obtained at 1.5 Tesla.

Different sequences will be planned:

* T2-weighted sequence \* 3D GRE multi-echo. This sequence will generate a R2\* maps of the whole brain.
* T1-weighted sequence in high resolution 3D. This sequence will allow the anatomical characterization of different brain structures and will help the normalization of T2\* in pictures an anatomical reference space.
* T2\* sequence 3D multiple gradient echo (Spoiled Gradient Recalled echo sequence). This sequence will measure the decay rate of NMR signal according to the echo time.
* 2D spin echo sequence T1-weighted for neuromelanin. This sequence view the substantia nigra and locus coeruleus (optional sequence).
* Optional Diffusion-weighted sequence. This sequence determines the movements of water molecule in the brain and to infer the main lines of connections between neurons (optional sequence).

The R2\* (1/T2\*) will be measured in three different regions of interest (substantia nigra, ventral tegmental area and the putamen) for the 2 MRI's in order to calculate ΔR2.

Eligibility criteria: Inclusion criteria for PATIENTS :

* Parkinson's Disease (UK Parkinson's Disease Society Brain Bank Criteria).
* No Deep Brain Stimulation (DBS).
* From 40 to 80 years old.

Inclusion criteria for HEALTHY CONTROL SUBJECTS :

- From 40 to 80 years old.

Exclusion criteria for PATIENTS :

* Dementia (MoCA \< 24).
* Atypical parkinsonism (MSA, PSP, ...).
* Severe current psychiatric or somatic disease.
* Iron treatments (Desferal® (deferoxamine), Ferriprox® (deferiprone) et Exjade® (deferasirox), Fumafer® (ferrous fumarate), Tardyferon® (ferrous sulfate (II)),...), Ferinject® (ferric carboxymaltose), Venofer® (iron sucrose),...).
* Contra-indication to MRI (claustrophobia, pace maker,...).

Exclusion criteria for HEALTHY CONTROL SUBJECTS :

* Neurological disease.
* Psychiatric or somatic disease.
* Dementia (MoCA \< 24).
* Iron treatments (Desferal® (deferoxamine), Ferriprox® (deferiprone) et Exjade® (deferasirox), Fumafer® (ferrous fumarate), Tardyferon® (ferrous sulfate (II)),...), Ferinject® (ferric carboxymaltose), Venofer® (iron sucrose),...).
* Contra-indication to MRI (claustrophobia, pace maker,...).

Primary outcomes:
measure: Change from baseline cerebral R2*
timeFrame: at 1 year
Secondary outcomes:
measure: Change from baseline Parkinson's disease clinical symptoms
timeFrame: at 1 year
measure: Change from baseline severity of Parkinson's disease
timeFrame: at 1 year
measure: Change from baseline activities of daily living
timeFrame: at 1 year
measure: Change from baseline freezing
timeFrame: at 1 year
measure: change from baseline cognitive function
timeFrame: at 1 year
measure: Change from baseline hyper and hypo dopaminergic symptoms scores
timeFrame: at 1 year
measure: Change from baseline sleepiness score
timeFrame: at 1 year
measure: Change from baseline autonomic functions score
timeFrame: at 1 year
measure: Change from baseline non-motor symptoms score
timeFrame: at 1 year
measure: Change from baseline apathy score
timeFrame: at 1 year
measure: Change from baseline depression score
timeFrame: at 1 year
measure: Change from baseline anxiety score
timeFrame: at 1 year
Other outcomes:
Locations:
city: Bordeaux
country: France
facility: Chu Pellegrin
city: Clermont-Ferrand
country: France
facility: CHU Clermont-Ferrand
city: Grenoble
country: France
facility: Chu Grenoble
city: Lille
country: France
facility: Chu Lille
city: Limoges
country: France
facility: Chu Dupuytren
city: Lyon
country: France
facility: Hôpital neurologique Pierre Wertheimer
city: Montpellier
country: France
facility: Chu Montpellier
city: Nancy
country: France
facility: Chu Nancy
city: Paris
country: France
facility: CHU Pitié Salpétrière
city: Paris
country: France
facility: Hôpital Henri Mondor
city: Potiers
country: France
facility: Chu Poitiers
city: Reims
country: France
facility: Chu Reims
city: Toulouse
country: France
facility: Chu Toulouse
Study officials:
affiliation: University Hospital, Clermont-Ferrand
name: Anna MARQUES
role: PRINCIPAL_INVESTIGATOR
References: