Clinical trial NCT04852653: A Prospective Feasibility Study Evaluating Extracellular Vesicles Obtained by Liquid Biopsy for Neoadjuvant Treatment Response Assessment in Rectal Cancer
Official title: A Prospective Feasibility Study Evaluating Extracellular Vesicles Obtained by Liquid Biopsy for Neoadjuvant Treatment Response Assessment in Rectal Cancer
Study type: OBSERVATIONAL
Overall status: RECRUITING
Conditions: Rectal Cancer
Liquid Biopsy
Keywords: Liquid biopsy
Rectal cancer
Extracellular vesicles
Exosome
ExoDNA
Lead sponsor: University Hospital, Bordeaux
Sponsor class: OTHER
First posted: 2021-04-21
Enrollment: 40
Sex: ALL
Minimum age (years): 18
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: no
Observational model: COHORT
Time perspective: PROSPECTIVE
Sampling method: NON_PROBABILITY_SAMPLE
Study population: Patients with adenocarcinoma of rectum histologically proven

Brief summary: Routine clinical and radiological assessment of response of patients with rectum cancer to neoadjuvant chemoradiotherapy does not allow accurate identification of complete pathological response, and leads to frequent false positive and negative results. Molecular markers reflecting the initial and post-nCRT status of the tumor would be ideal to select patients eligible for organ preservation. This project will test the detection of tumor extracellular vesicles (EVs) in liquid biopsy as a reliable marker for the identification of poor versus good responders to nCRT.

Detailed description: Neoadjuvant treatment (NT) followed by total mesorectum excision (TME) constitues the gold standard for locally advanced carcinoma of the low and middle rectum. When good clinical response to NT is obtain, strategies with organ preservation, including close surveillance without immediate surgery or transanal local excision, can spare patients with the highly morbid and functional cost of TME. Current assessment of good responders relies on the downstaging and/or downsizing of the tumor as evaluated by MRI imaging and clinical exam. This strategy is prone to errors in more than 1/3 cases. Better assessment of tumor response to NT would better select patients eligible for organ preservation strategies. This study will use the detection of tumor extracellular vesicles (EVs) in liquid biopsy to identify good response of rectum cancer to neoadjuvant treatment. Tumor EVs will be detected in the blood of the patients at different times of their regular management (before, during and after NT). Protein content or tumor DNA in EVs will be detected after tumor DNA sequencing in the primary biopsies. Response will be assessed according to tumor EV presence or absence in the blood. As EV quantification is possible, kinetics of their detection will help response assessment and patient follow-up.

Eligibility criteria: Inclusion Criteria:

* Adenocarcinoma of rectum histologically proven
* Location ≤ 10 cm from the anal margin

  * Stage T3T4, or T2 if low rectal cancer
  * With indication of radiochemotherapy alone or chemotherapy followed by chemoradiotherapy prior to total mesorectal surgery or transanal surgery
* No metastasis
* Operable patient
* Patient ≥ 18 years old
* Patient likely to receive radiation therapy and chemotherapy.
* No history of pelvic radiotherapy for any reason
* No history of chemotherapy unless it was more than 10 years ago
* Effective contraception for patients of childbearing age: male and non-menopausal women must agree to use two medically validated methods of contraception (one for the patient and the other for the partner) during treatment and less up to 6 months for men and 4 months for women after last treatment
* Patient information and obtaining free, informed and written consent, signed by the patient and his investigator.
* Affiliate subject or beneficiary of a social security scheme of a member country of the European Community (article L1121-11 of the Code of Public Health)

Exclusion Criteria:

* Tumor of the upper rectum (\> 10 cm from the anal margin)
* Metastatic disease

  . T1 stage tumor
* History of pelvic radiotherapy and chemotherapy
* Contraindication to chemotherapy and / or radiotherapy

  . Insufficient tumoral sample on initial rectal biopsy for performing molecular analysis of tumor by NGS.
* Other concomitant cancer or history of cancer other than treated in situ cervix cancer or basal cell or squamous cell carcinoma
* Pregnant woman, likely to be pregnant or breastfeeding

  * Symptomatic cardiac and / or coronary insufficiency
  * Severe renal impairment (creatinine clearance less than 30ml / min)
  * Full or partial deficiency in dihydropyrimidine dehydrogenase (DPD) (uracilemia ≥ 16 ng / ml)
  * Peripheral neuropathy\> grade 1
  * Treatment with St. John's Wort
  * Treatment with yellow fever vaccine
  * Treatment with prophylactic phenytoin
  * Treatment with sorivudine or its chemically analogues related, such as brivudine
  * Active infection or other serious underlying disease likely to prevent the patient from receiving treatment
  * Persons deprived of their liberty, or under measure of judicial protection (curatorship or guardianship) or incapable of giving their consent
  * Impossibility of submitting to the medical follow-up of the test for geographical, social or psychological reasons.

Primary outcomes:
measure: Positivity of the liquid biopsy
timeFrame: 6 months
Secondary outcomes:
measure: Prediction of the response of rectal cancer to neoadjuvant treatment
timeFrame: 6 months
measure: Genetic profile of the primary tumor predictive of the response to nCRT
timeFrame: 6 months
Other outcomes:
Locations:
city: Bordeaux
country: France
facility: CHU Bordeaux
Study officials:
References: