Clinical trial NCT04156113: Effect of an Exercise on Serum Uncoupling Protein-1 Level and the Role of Uncoupling Protein-1-3826 A/G Polymorphism
Official title: The Effect of an Acute Maximal Exercise on Serum Uncoupling Protein-1 Level and the Role of Uncoupling Protein-1-3826 A/G Polymorphism
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Uncoupling Protein-1
Polymorphism, Restriction Fragment Length
Irisin
Keywords: Beige fat cell
Fat tissue
Athletes
Sedentary people
Irisin
Lead sponsor: Eastern Mediterranean University
Sponsor class: OTHER
Collaborators: name: Ege University
First posted: 2019-11-07
Enrollment: 86
Sex: MALE
Minimum age (years): 18
Maximum age (years): 35
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: NON_RANDOMIZED
Intervention model: PARALLEL
Primary purpose: BASIC_SCIENCE

Brief summary: This study aims to determine the effect of an acute maximal exercise on serum uncoupling protein-1, irisin, interleukin 6 levels, and on blood lipid and lipoprotein concentrations. This study also evaluates the role of uncoupling protein-1-3826 A/G polymorphism on this eventual effect.

Therefore, this study hypothesizes that:

H01: Sedentary people and athletes' serum uncoupling protein 1, irisin, and interleukine 6 levels are different before and after an acute maximal exercise.

H02: Sedentary people and athletes' basal serum uncoupling protein 1 levels are associated with uncoupling protein 1-3826 A/G polymorphism.

H03: Uncoupling protein 1-3826 A/G polymorphism has a modifying role in the effect of maximal exercise on serum uncoupling protein 1 levels.

H04: Sedentary people and athletes' basal serum lipid and lipoprotein levels are associated with uncoupling protein 1-3826 A/G polymorphism.

H05: Uncoupling protein 1-3826 A / G polymorphism has a modifying role in the effect of maximal exercise on serum lipid and lipoprotein levels.


Eligibility criteria: Inclusion Criteria:

* Athletes group:

  * Must be basketball, or volleyball, or handball players.
  * Must be subjects who have been training regularly for at least 3 months.
* Sedentary group:

  \* Must be subjects who have not been training regularly for at least 3 months.
* For both groups:

  * Healthy.
  * Non obese (body mass index \< 30).
  * Aged between 18 and 35 years.

Exclusion Criteria:

* For both groups:

  * Being regular consumer of cigarettes, alcohol, drugs and antioxidant substances.
  * Being obese (body mass index \> 30).
  * Having an acute or chronic injury which is limiting physical performance.
  * Having a disease (anemia, infection...) detected from the biochemical analysis of the blood samples.
  * Being injured or sick during the study.

Primary outcomes:
measure: Change from the baseline serum uncoupling protein 1 level at 3 minutes post Yo-Yo intermittent recovery test (level 1)
timeFrame: 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)
measure: Change from the baseline serum irisin level at 3 minutes post Yo-Yo intermittent recovery test (level 1)
timeFrame: 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)
measure: Uncoupling protein-1-3826 A/G polymorphism determination
timeFrame: 15 minutes before the Yo-Yo intermittent recovery test (level 1)
Secondary outcomes:
measure: Change from the baseline serum interleukin-6 level at 3 minutes post Yo-Yo intermittent recovery test (level 1)
timeFrame: 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)
measure: Change from the baseline serum nitric oxide level at 3 minutes post Yo-Yo intermittent recovery test (level 1)
timeFrame: 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)
Other outcomes:
measure: Change from the baseline serum C reactive protein level at 3 minutes post Yo-Yo intermittent recovery test (level 1)
timeFrame: 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)
measure: Change from the baseline serum creatine kinase level at 3 minutes post Yo-Yo intermittent recovery test (level 1)
timeFrame: 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)
measure: Change from the baseline serum alanine aminotransferase level at 3 minutes post Yo-Yo intermittent recovery test (level 1)
timeFrame: 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)
measure: Change from the baseline serum aspartate aminotransferase level at 3 minutes post Yo-Yo intermittent recovery test (level 1)
timeFrame: 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)
measure: Change from the baseline hemogram test at 3 minutes post Yo-Yo intermittent recovery test (level 1)
timeFrame: 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)
measure: Change from the baseline at serum glucose level at 3 minutes post Yo-Yo intermittent recovery test (level 1)
timeFrame: 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)
measure: Change from the baseline serum urea level at 3 minutes post Yo-Yo intermittent recovery test (level 1)
timeFrame: 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)
measure: Change from the baseline serum creatinine level at 3 minutes post Yo-Yo intermittent recovery test (level 1)
timeFrame: 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)
measure: Change from the baseline serum total cholesterol level at 3 minutes post Yo-Yo intermittent recovery test (level 1)
timeFrame: 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)
measure: Change from the baseline serum low density lipoprotein level at 3 minutes post Yo-Yo intermittent recovery test (level 1)
timeFrame: 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)
measure: Change from the baseline serum high density lipoprotein level at 3 minutes post Yo-Yo intermittent recovery test (level 1)
timeFrame: 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)
measure: Change from the baseline serum triglyceride level at 3 minutes post Yo-Yo intermittent recovery test (level 1)
timeFrame: 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)
measure: Change from the baseline serum total antioxidant level at 3 minutes post Yo-Yo intermittent recovery test (level 1)
timeFrame: 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)
measure: Change from the baseline serum total oxidant level at 3 minutes post Yo-Yo intermittent recovery test (level 1)
timeFrame: 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)
measure: Change from the baseline heparin plasma thiobarbituric acid reactive substances level at 3 minutes post Yo-Yo intermittent recovery test (level 1)
timeFrame: 15 minutes before and 3 minutes after the Yo-Yo intermittent recovery test (level 1)
Locations:
city: Izmir
country: Turkey
facility: Ege University Faculty of Sport Sciences
Study officials:
affiliation: Ege University, Faculty of Sports Science
name: Faruk Turgay, Assoc. Prof
role: STUDY_CHAIR
References: