Clinical trial NCT06045234: Temporal Variation of the Exposure to Pesticides in the Employees of the Limoges University Hospital
Official title: Temporal Variation of the Exposure to Pesticides in the Employees of the Limoges University Hospital
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Toxicology
Keywords: Pesticides
general population
measurement
Lead sponsor: University Hospital, Limoges
Sponsor class: OTHER
First posted: 2023-09-21
Enrollment: 300
Sex: ALL
Minimum age (years): 18
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: yes
Allocation: NA
Intervention model: SINGLE_GROUP
Primary purpose: DIAGNOSTIC

Brief summary: In a sample of 300 employees of the Limoges University Hospital, it is proposed to explore the intraindividual and interindividual variability of urinary concentrations of glyphosate and 320 other pesticides. The employees will fill a questionary in which will allow to explore the relationships between the concentrations, lifestyle and dietary habits.

Detailed description: The general population is exposed to pesticides through the ingestion of food and water. The presence of pesticides in human urine has been demonstrated in numerous studies. Conventionally, biological monitoring studies use an undifferentiated urine sample or the first morning urine. The reliability of measuring a single-point urinary concentration to represent the profile of pesticide exposure in individuals over time is, however, questionable. Rare studies reporting intra-individual outcomes of pesticide exposure have been conducted in specific populations, such as children and pregnant women.

It is proposed to carry out a descriptive biomonitoring study of the urinary concentration of approximately 320 pesticides in a sample of the general population. Among these, the study will focus on glyphosate.

The originality of our study is:

* (i) Exploration of exposure to the main pesticides in a sample of 300 people by measuring urinary concentration by specific and sensitive methods of LC-MS/MS and GC-MS/MS; These 300 subjects will be hospital staff of the Limoges University Hospital.
* (ii) Exploration of the intra-individual variability of exposure by repeating measurements in urine samples taken over a 24-hour period and/or several times over the course of a week;
* (iii) Determination of the frequency of subjects who have potentially been exposed to doses higher than the acceptable daily intake (in particular for glyphosate) (iv) The association of these exposure values with data from a self-administered questionnaire in order to identify the determinants of this exposure
* (V) Estimating the predictability/reliability of a single urine sample to represent this exposure of individuals/populations over time.

Eligibility criteria: Inclusion Criteria:

* Adult 18 years and over.
* Employed by the University Hospital of Limoges.
* Having consented to participate in the study after being informed.

Exclusion Criteria:

- Adult with a professional activity during which he is required to handle or come into contact with glyphosate

Primary outcomes:
measure: glyphosate overconsumption
timeFrame: 1 week
Secondary outcomes:
measure: Identification of determinant of exposure to glyphosate
timeFrame: 1 week
measure: Identification of determinant of exposure to glyphosate
timeFrame: 1 week
measure: Identification of determinant of exposure to glyphosate
timeFrame: 1 week
measure: Identification of determinant of exposure to glyphosate
timeFrame: 1 week
measure: Identification of determinant of exposure to glyphosate
timeFrame: 1 week
measure: Coefficient of variation of the glyphosate concentrations
timeFrame: 1 week
measure: Intra-individual variability of urinary glyphosate concentrations
timeFrame: 1 week
measure: Impact of urine sample collection schedule on glyphosate concentration measurement
timeFrame: 1 week
measure: Identification of determinants of exposure to a panel of around 320 pesticides
timeFrame: 1 week
measure: Identification of determinants of exposure to a panel of around 320 pesticides
timeFrame: 1 week
measure: Identification of determinants of exposure to a panel of around 320 pesticides
timeFrame: 1 week
measure: Identification of determinants of exposure to a panel of around 320 pesticides
timeFrame: 1 week
measure: Identification of determinants of exposure to a panel of around 320 pesticides
timeFrame: 1 week
measure: Intra-individual variability of urinary 320 pesticides concentrations
timeFrame: 1 week
measure: Impact of urine sample collection schedule on 320 pesticides concentration measurement
timeFrame: 1 week
Other outcomes:
Locations:
city: Limoges
country: France
facility: CHU de Limoges
Study officials:
References: