Clinical trial NCT06426147: L-citrulline to Improve Adverse Outcomes in Admitted Children (EChiLiBRiST, Clinical Trial 2, Inpatients)
Official title: A Randomised, Double-blind, Placebo-controlled Trial of L-Citrulline Oral Supplementation to Improve Short and Long-term Outcomes of Admitted Febrile Paediatric Patients With Biomarker-determined High-risk of Adverse Outcomes
Study type: INTERVENTIONAL
Overall status: NOT_YET_RECRUITING
Phase: NA
Conditions: Infectious Disease
Infections
Child, Only
Lead sponsor: Barcelona Institute for Global Health
Sponsor class: OTHER
First posted: 2024-05-23
Enrollment: 2200
Sex: ALL
Minimum age (years): 0
Maximum age (years): 60
Standard ages: CHILD
Accepts healthy volunteers: no
Allocation: RANDOMIZED
Intervention model: PARALLEL
Primary purpose: TREATMENT

Brief summary: In low and middle-income countries, children admitted to hospital are not similarly ill, and do not all have a comparable prognosis. In fact, understanding at first encounter their risk of developing adverse outcomes (including mortality) could allow a more focused management and the tailoring of specific interventions to decrease in hospital mortality, and post discharge adverse longer-term outcomes. This clinical trial, part of the EChiLiBRiST larger project ("Development and validation of a quantitative point-of-care test for the measurement of severity biomarkers to improve risk stratification of fever syndromes and enhance child survival") has the two-fold objective of:

1. Assessing whether a POINT-OF-CARE rapid triaging test (PoC RTT) based on the quantitative measurement at the bedside of the "prognostic" biomarker sTREM-1 (soluble-triggering receptor expressed on myeloid cells 1) can reliably identify those admitted children with a higher risk of adverse outcomes; and
2. Assessing whether the therapeutic intervention (the L-arginine precursor, L-Citrulline, key in the nitric oxide biosynthesis), administered orally for 28 days to those children aged 1-\<60 months identified as "moderate-to-high risk" by the prognostic biomarker can improve outcomes as compared to those receiving an indistinguishable placebo.

This second objective will be assessed in a prospective multi-country, multi-site, individually randomised, two-arm, placebo-controlled, double blind clinical trial involving \~888 children 1-\<60m of age admitted to hospital and determined to be at high risk of adverse outcomes by their baseline sTREM-1 levels. The trial will compare the efficacy of a twice-daily dose of L-citrulline syrup vs placebo (200-300mg/kg/day depending on weight-band; for 28 days) in reducing adverse outcomes in children with severe disease. The trial will be running independently but in parallel in two high-mortality settings in Mozambique and in Ethiopia.

Detailed description: Children admitted to hospital and meeting the study eligibility criteria who are 0-\<60 months of age will be eligible for study inclusion, and for initial biomarker screening using the study-designed rapid triaging PoC test, based on the measurement of sTREM-1. Study participants aged 1m-\<5 years of age with sTREM-1 values classified as moderate (i.e., "yellow") or high-risk (i.e., "red") in the traffic light risk-stratification system will be randomly allocated (1:1) to receive L-Cit intervention or placebo. All study participants will be followed for 6 months, with study visits at the study hospitals or at home or via phone communication after discharge at day 3, day 5, day 7, day 28, and month 6. The study primary outcome will be "adverse disease outcome", defined as a composite of mortality, incident neurological sequelae, major adverse kidney event at discharge, need for organ support, clinical shock, coma, severe respiratory distress or need for readmission within 28 days after recruitment.

Eligibility criteria: Inclusion Criteria:

* Enrolled in the initial prognostic screening component.
* Sick children with fever (axillary temperature\>37.5ºC) or a history of fever (within the preceding 72h) or with suspected severe disease.
* 1m-\<60 months of age.
* With an indication for admission, or having already been admitted to hospital due to their illness.
* With an sTREM-1 PoC result classifying their disease as of "moderate-high risk" ("yellow" or "red") upon study recruitment and within D3.
* Residents in the study area or willing to be contacted and traced during the study duration.
* Willing to sign an informed consent document.
* Willing to undergo and adhere to study procedures as explained in the IC document.

Exclusion Criteria:

* Admission to hospital for social reasons (and not on account of their disease).
* Children for which informed consent document has not been signed.
* Known allergy or contraindication to any of the study supplements including lactose intolerance or observing a lactose-free diet.
* Concurrent participation in any other clinical trial.
* Patient under NPO or "nothing by mouth" prescription .
* Contraindication for the insertion of a nasogastric tube (NGT) of for the enteral administration of drugs through the NGT in children who cannot tolerate by mouth.
* Critically sick patient whose prognosis is considered by the clinical researcher as fatal outcome in the following hours after screening.
* Any other condition determined by the investigators that makes it unlikely that the participant would complete the follow up until day 28 of study.

Primary outcomes:
measure: Adverse disease outcome
timeFrame: Up to day 28
Secondary outcomes:
measure: Mortality
timeFrame: Up to day 28
measure: Incident neurological sequelae
timeFrame: Up to day 28
measure: Major adverse kidney event
timeFrame: Up to day 28
measure: Need for organ support
timeFrame: Up to day 28
measure: Clinical shock
timeFrame: Up to day 28
measure: Severe respiratory distress
timeFrame: Up to day 28
measure: Coma
timeFrame: Up to day 28
measure: Need for readmission
timeFrame: Up to day 28
measure: Median duration of antibiotic treatment
timeFrame: Up to day 28
measure: Oxygen requirement
timeFrame: Up to day 28
measure: Radiological pneumonia
timeFrame: Up to day 28
measure: Hypoxemia (Sp02 <90%)
timeFrame: Up to day 28
measure: Lenght of hospitalisation
timeFrame: Up to day 28
measure: Mortality
timeFrame: Up to month 6
measure: Secondary consultation or hospitalisations
timeFrame: Up to month 6
measure: Proportion of participants with serious adverse events
timeFrame: Up to month 6
measure: Proportion of participants with suspected unexpected serious adverse reactions
timeFrame: Up to month 6
measure: Proportion of participants with adverse events
timeFrame: Up to day 30
Other outcomes:
measure: Concentration of circulating mediators of host immune and endothelial function, inflammation, intestinal barrier function, and neuronal damage
timeFrame: Up to day 7
measure: Lactate levels
timeFrame: Up to day 3
measure: Levels of markers of kidney function
timeFrame: Up to day 7
measure: PoC-RTT prognostic performance
timeFrame: Up to day 28
measure: Prognostic performance of a larger panel of biomarkers
timeFrame: At baseline
Locations:
Study officials:
affiliation: Barcelona Institute for Global Health
name: Quique Bassat, Prof
role: PRINCIPAL_INVESTIGATOR
References: