Clinical trial NCT02417519: Coffee Roasting and Glucose Tolerance
Official title: Acute Effects of Light and Dark Roasted Coffee on Glucose Tolerance: A Randomized, Controlled Crossover Trial in Healthy Volunteers
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Oral Glucose Tolerance
Keywords: Coffee
Chlorogenic acid
Insulin
Appetite
Metabolomics
Lead sponsor: University of Copenhagen
Sponsor class: OTHER
First posted: 2015-04-15
Enrollment: 11
Sex: ALL
Minimum age (years): 18
Maximum age (years): 65
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: CROSSOVER
Primary purpose: PREVENTION

Brief summary: Chlorogenic acid (CGA) in coffee may lower the postprandial glucose response. CGA is destroyed by dark roasting. In a controlled crossover trial, 11 healthy fasted volunteers consumed 300 mL of either light (LIR) or dark (DAR) roasted coffee, or water, followed 30 min later by a 75-g oral glucose tolerance test (OGTT). Plasma glukose and insulin, appetite, and plasma and urine metabolic profiles will be analysed. The primary aim is to investigate whether roasting affects the postprandial glucose area under the curve (AUC).

Detailed description: Epidemiologic evidence suggests that coffee consumption is associated with a lower risk of type 2 diabetes. Coffee contains caffeine and several other components that may modulate glucose regulation. The chlorogenic acids (CGA) in coffee have been indicated as constituents that may help to normalize the acute glucose response after a carbohydrate challenge. The aim of this study was to investigate whether two coffee beverages that differ in CGA content due to different roasting degrees will affect glucose regulation differently.

In a controlled crossover trial, 11 healthy fasted volunteers consumed 300 mL of either light roasted (LIR) or dark roasted (DAR) coffee, or water, followed 30 min later by a 75-g oral glucose tolerance test (OGTT). Blood samples were drawn at baseline, 30, 60 and 120 min. Differences in glucose and insulin responses and insulin sensitivity index (ISI) were analyzed. The CGA and caffeine contents in the coffees were analyzed using UPLC-MS/MS.

Eligibility criteria: Inclusion Criteria:

Body mass index (BMI) of 18.0-30.0 kg/m2 and with no known diseases

Exclusion Criteria:

* systemic infections,
* psychiatric or metabolic disorders,
* known food allergies or intolerances related to the products used in the study (e.g. dairy or gluten),
* ongoing or former drug abuse,
* high intake of alcohol (defined as a weekly intake of \>7 units for women and \> 14 units for men),
* pregnancy or ongoing planning of pregnancy,
* vegetarianism or veganism,
* participation in other scientific studies during the study period, and
* blood donation during - or in the month leading up to - the study period

Primary outcomes:
measure: Plasma glucose Area Under the Curve (Glucose AUC)
timeFrame: 0-120 min
Secondary outcomes:
measure: Plasma glucose concentrations
timeFrame: 30, 60 and120 min
measure: Serum Insulin Area Under the Curve
timeFrame: 0-120 min
measure: Serum insulin concentrations
timeFrame: 30, 60 and120 min
measure: ISI(0-120)
timeFrame: 0-120min
measure: Metabolic profile in plasma
timeFrame: 0-24 hours
measure: Metabolic profile in urine
timeFrame: 0-24 hours
Other outcomes:
Locations:
city: Frederiksberg
country: Denmark
facility: Department of Nutrition, Exercise and Sports, University of Copenhagen
Study officials:
affiliation: University of Copenhagen
name: Lars O Dragsted, PhD
role: PRINCIPAL_INVESTIGATOR
References: