Clinical trial NCT05295836: Effects of Acute Glycerol Ingestion on Performance, Metabolic and Biochemical Markers in International Athletes
Official title: Effects of Acute Glycerol Ingestion on Performance, Metabolic and Biochemical Markers in International Athletes
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Performance Enhancing Product Use
Change, Body Weight
Change, Body Temperature
Antioxidative Stress
Lactate Blood Increase
Thermal Sensation Disorders
Keywords: Power
Performance
Thermography
Metabolism
Endurance Sport
Ergogenic Aids
Oxygen uptake
Hydration
Hyperhydration
Lead sponsor: Universidad Católica San Antonio de Murcia
Sponsor class: OTHER
First posted: 2022-03-25
Enrollment: 4
Sex: MALE
Minimum age (years): 25
Maximum age (years): 35
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: CROSSOVER
Intervention model description: A randomized crossover study will be conducted, in which 4 international walkers will perform 3 different conditions: CON) water intake of 26 mL/kg/weight at 16°C; PLA) 26 mL/kg/weight + placebo at 28°C and GLY) intake of 1.2 g glycerol + 26 mL/kg/weight at 28°C.
Primary purpose: TREATMENT

Brief summary: This clinical study evaluates the acute effect of glycerol ingestion on performance (power output), weight, urine specific gravity, biochemical (antioxidants and lactate) and metabolic (indirect calorimetry) markers in international athletes in hot conditions. The investigators hypothesized that acute glycerol ingestion can prevent performance loss (power generated at submaximal intensity) in hot conditions. To justify this hypothesis, the investigators will measure the aforementioned markers, which could establish a cause-effect relationship between acute glycerol intake and decreased performance loss in hot conditions.

Detailed description: All participants will be informed about the procedures and provide signed informed consent. The study will be conducted according to the guidelines of the Helsinki Declaration for Human Research and the protocol was approved by the Ethics Committee/Institutional Review Catholic University of Murcia (Code: CE091802).

Study design

This is a randomized crossover study. There are 3 types of intervention: water intake of 26 mL/kg/weight at 16°C (CON), 26 mL/kg/weight + placebo at 28°C (PLA) and intake of 1.2 g glycerol + 26 mL/kg/weight at 28°C (GLY). In addition, athletes will be instructed to take a individualized diet in relation to macronutrients (verified by dietary record) and they will follow their adjusted training schedule so that there would be no effect on the tests. Participants in both groups will be instructed not to consume supplements 3 weeks prior to the start of the study.

Procedures

Participants will visit the laboratory on five occasions. Visit 1 consists of a medical examination and a blood draw to determine health status. At visit 2, after a dinner the night before and a standard breakfast, an incremental test will be performed to determine the exercise zones in the rectangular test. At visits 3, 4 and 5, a record of the diet (individually set) will be performed regarding the former 7 days, followed by a rectangular treadmill test.

Visit 1:

Health status blood test: A general blood test will be performed. The blood will be drawn through a vein. (Fasting) Medical examination: A medical history of family and personal history, an electrocardiogram (ECG) at rest and a medical examination (auscultation, blood pressure, etc.) will be carried out to certify that the person is healthy and is not at risk to participate in the study. (Fasting)

Visits 2:

Incremental test with final ramp test will be performed on a cycle ergometer using a metabolic cart (Metalyzer 3B. Leipzig, Germany) to determine maximal fat oxidation zone (FatMax), ventilatory thresholds 1 (VT1) and 2 (VT2) and maximal oxygen consumption (VO2max). Participants will begin cycling at 35W for 2 min, increasing then by 35W every 2 min until RER\>1.05, initializing then the final ramp (+35W·min-1) until exhaustion.

Visits 3:

The rectangular test will be performed on a treadmill using the speed values obtained in the incremental test (15 sets x 2 min at 95% with 2:30 recovery at 85% of maximum speed between sets). Cardiorespiratory variables (VO2, VCO2, VO2R, VE/VO2, VE/VCO2, VE/VCO2, respiratory coefficient, respiratory frequency, carbohydrate oxidation (CHO), fat oxidation (FAT), running economy and energy expenditure) will be determined in the 1st, 5th, 10th and 15th series.

For power measurement, each participant will wear the Stryd power meter, which is an inertial sensor (9.1 g) mounted on the foot, firmly attached to the shoe and according to the manufacturer's recommendations. The device stores at a sampling rate of 1 Hz the following variables: estimated power (PO), pace, cadence, leg sprint stiffness (LSS), form power, form power ratio, ground contact time (GCT), vertical oscillation, stride length. These variables will then be determined in the 1st, 5th, 10th and 15th series of the rectangular test. According to information from the Stryd team, the device is operational out of the box and should not require any calibration, accepting a measurement error of 3 percent. Participants will fill in their height and body mass prior to use, which is required for PO estimation. The firmware version that will be used is 2.1.16 (released in August 2020).

For lactate and antioxidant measurements, a Lactate Pro 2 meter and eBQC lab will be used on finger capillary blood at rest, after the 5th, 10th and after completion of the rectangular test.

For body temperature, the investigators will use an infrared thermographic camera (FLIR) to measure heat distribution (head, trunk, lower and upper limbs in both anterior and posterior aspects of the body).

Urine Samples Urine will be collected from the intake of water or water + glycerol or water + placebo until the start of the rectangular test and up to one hour after the rectangular test. The volume of urine excreted pre and post rectangular test will be recorded and the urine specific gravity will be analyzed.

Eligibility criteria: Inclusion Criteria:

* Be an international athlete with the Spanish national athletics team.

Exclusion Criteria:

* Are smokers or regular alcohol drinkers,
* Have a metabolic, cardiorespiratory or digestive pathology or anomaly,
* Have an injury in the prior 6 months
* Are supplementing or medicating in the prior 2 weeks
* Have non-normal values in the blood analysis parameters.

Primary outcomes:
measure: Body weight
timeFrame: Throughout study completion, an average of 3 weeks
measure: Urine specific gravity
timeFrame: Throughout study completion, an average of 3 weeks
measure: Power output
timeFrame: Throughout study completion, an average of 3 weeks
measure: Body temperature
timeFrame: Throughout study completion, an average of 3 weeks
Secondary outcomes:
measure: Lactate
timeFrame: Throughout study completion, an average of 3 weeks
measure: Measurement of fast- and slow-acting antioxidants (Q1 and Q2) and total antioxidant capacity by a portable analyzer eBQC0101
timeFrame: Throughout study completion, an average of 3 weeks
measure: Heart rate
timeFrame: Throughout study completion, an average of 3 weeks
measure: VO2
timeFrame: Throughout study completion, an average of 3 weeks
measure: CO2
timeFrame: Throughout study completion, an average of 3 weeks
measure: RER
timeFrame: Throughout study completion, an average of 3 weeks
measure: VE
timeFrame: Throughout study completion, an average of 3 weeks
measure: FAT ox
timeFrame: Throughout study completion, an average of 3 weeks
measure: HC ox
timeFrame: Throughout study completion, an average of 3 weeks
measure: FR
timeFrame: Throughout study completion, an average of 3 weeks
measure: EE
timeFrame: Throughout study completion, an average of 3 weeks
measure: V'E/V'O2
timeFrame: Throughout study completion, an average of 3 weeks
measure: V'E/V'CO2
timeFrame: Throughout study completion, an average of 3 weeks
measure: VO2/R
timeFrame: Throughout study completion, an average of 3 weeks
measure: Cadence
timeFrame: Throughout study completion, an average of 3 weeks
measure: Leg spring stiffness
timeFrame: Throughout study completion, an average of 3 weeks
measure: Form power ratio
timeFrame: Throughout study completion, an average of 3 weeks
measure: Form power
timeFrame: Throughout study completion, an average of 3 weeks
measure: Ground contact time
timeFrame: Throughout study completion, an average of 3 weeks
measure: Vertical oscillation
timeFrame: Throughout study completion, an average of 3 weeks
measure: PO/R
timeFrame: Throughout study completion, an average of 3 weeks
Other outcomes:
Locations:
city: La Ñora
country: Spain
facility: Research Center for High Performance Sport. Catholic University of Murcia
state: Murcia
Study officials:
affiliation: Research Center for High Performance Sport. Catholic University of Murcia
name: Francisco Javier Martínez Noguera, PhD
role: STUDY_CHAIR
References: