Clinical trial NCT03577145: Impact of Inulin on Production of Phenolic Acids From Tomato Onion and Lovage Soup
Official title: Impact of Inulin on Production of Phenolic Acids From Tomato Onion and Lovage Soup
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Healthy Adults
Keywords: Bioavailability
Polyphenols
Dietary fibre
Phenolic acids
Lead sponsor: University of Glasgow
Sponsor class: OTHER
Collaborators: name: Scottish Universities Environmental Research Centre
First posted: 2018-07-05
Enrollment: 15
Sex: ALL
Minimum age (years): 20
Maximum age (years): 70
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: CROSSOVER
Intervention model description: Acute human bioavailability studies with a cross-over design. Subjects will be given a soup or drink in one of three combinations

1. Tomato onion and lovage soup
2. Inulin
3. Mixture of tomato onion and lovage soup with inulin
Primary purpose: BASIC_SCIENCE

Brief summary: This is an acute human bioavailability study in self-reported healthy participants aged 20-70 years old. The investigators hypothesize that combination of polyphenolics from a soup rich in rutin and quercitin and the non-digestible carbohydrate (NDC) inulin will increase the production of phenolic acids by bacteria in the human colon and these will be detected in urine. Participants will attend for three arms in a randomized order: Tomato, onion and lovage soup (high polyphenol food), Inulin (NDC) or Mixture of tomato, onion and lovage soup and inulin.

During each feeding study, urine, blood and stool samples will be collected at regular intervals for the duration of 24 hrs after consumption of test food. Participants will be asked to follow a low polyphenol diet for 2 days prior to the feeding study.

Detailed description: Polyphenol rich plant foods have been associated with several health benefits but their bioavailability is generally low. The majority of plant polyphenols are poorly absorbed in the small intestine and enter the colon where the colonic microbiota metabolise them to release a range of phenolic acids, which are now thought to be the main bioactive components related to the reduction in disease risk. Very little is known about the impact of other constituents of the diet on the metabolism and bacterial catabolism of these polyphenols.

Colonic bacteria are key agents in the release of the bioactive molecules from polyphenols but also ferment non-digestible carbohydrates (NDC) such as inulin to short chain fatty acids. It is likely that there are key interactions in the colonic bacterial metabolism of NDC and phenolics. The investigators hypothesize that combination of polyphenolics (in onions, tomatoes and lovage) with inulin (NDC) will increase the urinary output of bioactive phenolic acids.

Eligibility criteria: Inclusion Criteria:

• Self-reported healthy adults

Exclusion Criteria:

* Antibiotic use within the last 3 months
* Identified gastro-intestinal diseases
* On prescribed medication other than the contraceptive pill
* Pregnant or breastfeeding.
* Diagnosed as anaemic
* Allergic to paracetamol or any food

Primary outcomes:
measure: Phenolic acids bioavailability
timeFrame: 0-24 hrs
Secondary outcomes:
measure: Glycaemic measurements
timeFrame: 0-8 hrs
measure: Appetite hormones measurements
timeFrame: 0-8 hrs
measure: Mouth to caecum transit time
timeFrame: 0-8 hrs
measure: Gastric emptying time
timeFrame: 0-6 hrs
Other outcomes:
Locations:
city: Glasgow
country: United Kingdom
facility: School of Medicine, Nursing and Dentistry, College of MVLS, University of Glasgow
state: Lanarkshire
Study officials:
affiliation: University of Glasgow
name: Christine Edwards, PhD
role: PRINCIPAL_INVESTIGATOR
References:
citation: Russell W, Duthie G. Plant secondary metabolites and gut health: the case for phenolic acids. Proc Nutr Soc. 2011 Aug;70(3):389-96. doi: 10.1017/S0029665111000152.
citation: Roowi S, Mullen W, Edwards CA, Crozier A. Yoghurt impacts on the excretion of phenolic acids derived from colonic breakdown of orange juice flavanones in humans. Mol Nutr Food Res. 2009 May;53 Suppl 1:S68-75. doi: 10.1002/mnfr.200800287.
citation: Tamura M, Nakagawa H, Tsushida T, Hirayama K, Itoh K. Effect of pectin enhancement on plasma quercetin and fecal flora in rutin-supplemented mice. J Food Sci. 2007 Nov;72(9):S648-51. doi: 10.1111/j.1750-3841.2007.00557.x.