Clinical trial NCT04368806: A Phase 2b/3a Study to Evaluate the Efficacy and Safety of JointStem in Patients Diagnosed as Knee Osteoarthritis
Official title: A 48-Weeks, Phase 2b/3a, Double-Blind, Randomized, Placebo Controlled, Multi-center, Superiority Study to Evaluate the Efficacy and Safety of JointStem, Autologous Adipose Tissue Derived Mesenchymal Stem Cells in Patients Diagnosed as Knee Osteoarthritis
Study type: INTERVENTIONAL
Overall status: UNKNOWN
Phase: PHASE2
PHASE3
Conditions: Osteoarthritis, Knee
Lead sponsor: Nature Cell Co. Ltd.
Sponsor class: INDUSTRY
First posted: 2020-04-30
Enrollment: 140
Sex: ALL
Minimum age (years): 18
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: no
Allocation: RANDOMIZED
Intervention model: PARALLEL
Primary purpose: TREATMENT

Brief summary: This study is a double-blind, randomized, placebo controlled, multi-center, superiority study to evaluate the efficacy and safety of JointStem, autologous adipose tissue derived mesenchymal stem cells (AdMSC), in patients diagnosed as knee osteoarthritis. Following a 2-week screening period, approximately 140 subjects will be randomly assigned into one of the following two arms in a 1:1 ratio (1 JointStem : 1 Placebo). After each subject completes 48-Weeks visit (Visit 8) and the data management team confirms all individual data have no issue, the individual database will be locked and the blinding will be open for the statistical analysis.

Detailed description: Study Procedures:

* Visit 1 (Week -7) - Screening
* Visit 2 (Week -5) - Baseline and Randomization (Lipoaspiration)
* Visit 3 (Week 0) - Treatment (Intra-articular injection)
* Visit 4 (Week 4) - 4 weeks follow-up
* Visit 5 (Week 12) - 12 weeks follow-up
* Visit 6 (Week 24) - 24 weeks follow-up
* Visit 7 (Week 36) - 36 weeks follow-up
* Visit 8 (Week 48) - 48 weeks follow-up (End of Study)

Eligibility criteria: Inclusion Criteria:

* Subject who had osteoarthritis of knee diagnosed (according to the clinical and American College of Rheumatology Criteria)
* Subject who has ≥ 34 on WOMAC function score at Screening and Baseline
* Subject who has knee pain ≥ 70 mm for one knee out of 100 mm on VAS (Visual Analog Scale) at Screening and Baseline
* Subject who has radiographic evidence of grade 3 osteoarthritis in the study knee based on the Kellgren and Lawrence radiographic criteria.
* Subjects whose knee pain persists for at least 12 weeks (about 3 months) prior to screening and does not improve symptoms with non-operative treatment options
* Subject who is willing to discontinue all pain medications for osteoarthritis except rescue medication (\< acetaminophen 3.25 g per day) at least 72 hours prior to screening and throughout the duration of study
* Subject who is willing and able to give written informed consent for participation in the study

Exclusion Criteria:

* Subject who has Body Mass Index (BMI) \> 35 kg/m2
* Subject judged by the investigator to have a history of clinically significant disease
* Subject who has any of following clinically significant disease
* Autoimmune diseases
* Paget's disease, ochronosis, acromegaly, hemochromatosis, or Wilson's disease
* Genetic diseases (hyperkinesia, collagen gene abnormality, etc.)
* Inflammatory joint disorders (e.g. rheumatoid inflammation)
* Infectious joint disorders (e.g. septic arthritis)
* Other joint disorders (e.g. gout, recurrent pseudogout, articular fracture, primary osteochondrosis, villonodular synovitis)
* Subject who has any history of cancer or currently receiving treatment for a current cancer diagnosis
* Subject who is positive in pathogenic test (HIV, viral hepatitis, or syphilis)
* Subject who has heart diseases (myocardial infarction, coronary artery bypass graft surgery, arrhythmia, or other serious heart diseases) or has history of heart diseases within 6 months prior to Screening
* Subject who have received any intra-articular therapy in any joint within 6 months prior to Screening, or surgery on the relevant knee including articular endoscopic procedures within 6 months prior to Screening
* Subject who has history of prolotherapy, or platelet rich plasma injection within 6 months prior to Screening
* Subject who have received long-acting hyaluronic acid injection (e.g. Synvisc-One®, etc.) within 6 months prior to Screening
* Subject who has history of stem cell therapy
* Subject who have significant lab abnormalities for the following parameters (If the value is within 10% of the listed laboratory exclusion criterion value and the value is considered not to be clinically significant by the investigator, the subject can be considered for enrollment):

  * Serum ALT and AST \> 2 x upper limit of normal
  * Serum creatinine out of normal range
  * PT/INR out of normal range
  * Hemoglobin \< 10 g/dL for female subject and hemoglobin \< 11 g/dL for male subject
  * Platelets out of normal range
* Subject for whom the investigator judges the lipoaspiration can cause any problem
* Subject who has history of local anesthetic allergy
* Subject who has taken anti-inflammatory drugs (prescription and non-prescription NSAIDs), symptomatic slow acting drugs (glucosamine, chondroitin sulfate, diacerhein etc.), or oral steroids (prednisone etc.) within 14 days prior to Screening (however, those who undergo a 14-day wash-out period can participate.)
* Subject who is an active drug/alcohol abuser
* Pregnant or breast-feeding women, or women or men who are not using appropriate method of contraception (appropriate method includes hormones, bilateral tubal ligation, and barrier method with spermicide, and intra-uterine device for women and vasectomy and barrier method with spermicide for men; subjects should agree to use appropriate method)
* Subject who is enrolled in any other clinical trials within 3 months from Screening
* Subject who received the COVID-19 vaccines within 1 week from Visit 2 (Lipoaspiration, baseline)
* Subject who the principal investigator considers inappropriate for the study due to any other reasons than those listed above

Primary outcomes:
measure: Western Ontario and McMaster Universities Arthritis Index (WOMAC) function Score
timeFrame: Baseline and Week 48
measure: Visual Analog Scale (VAS) score
timeFrame: Baseline and Week 48
Secondary outcomes:
measure: Western Ontario and McMaster Universities Arthritis Index (WOMAC) subscale (Function)
timeFrame: Week 12, 24 and 36
measure: Western Ontario and McMaster Universities Arthritis Index (WOMAC) subscale (Pain)
timeFrame: Week 12, 24, 36, and 48
measure: Total Western Ontario and McMaster Universities Arthritis Index (WOMAC) score
timeFrame: Week 12, 24, 36, and 48
measure: Visual Analog Scale (VAS) score
timeFrame: Week 12, 24 and 36
measure: International Knee Documentation Committee (IKDC) score
timeFrame: Week 12, 24, 36, and 48
measure: 36-Item Short Form health survey questionnaires (SF-36) score
timeFrame: Week12, 24, 36, and 48
measure: Kellgren-Lawrence grade
timeFrame: Week 48
measure: Number and amount of rescue medication use
timeFrame: Week 12, 24, 36, and 48
Other outcomes:
Locations:
city: El Cajon
country: United States
facility: TriWest Research Associates
state: California
city: La Mesa
country: United States
facility: BioSolutions Clinical Research Center
state: California
city: Napa
country: United States
facility: Neurovations Research
state: California
city: Newport Beach
country: United States
facility: Newport Therapeutics
state: California
city: Santa Monica
country: United States
facility: Source Healthcare
state: California
Study officials:
References: