Clinical trial NCT04629313: GCF Levels of IL-1Beta, MIP-1 Alfa and G-CSF in Gingivitis and Stage I-II-III-IV Periodontitis Patients
Official title: Usak University Faculty of Dentistry
Study type: OBSERVATIONAL
Overall status: COMPLETED
Conditions: Periodontitis
Keywords: periodontal disease
periodontitis
cytokines
Lead sponsor: Uşak University
Sponsor class: OTHER
First posted: 2020-11-16
Enrollment: 120
Sex: ALL
Minimum age (years): 20
Maximum age (years): 65
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: yes
Observational model: CASE_CONTROL
Time perspective: PROSPECTIVE
Sampling method: PROBABILITY_SAMPLE
Study population: individuals who have periodontal disease will be selected

Brief summary: Periodontal diseases are chronic diseases that occur as a result of a violation of the balance between microbial dental plaque and the host response. Gingivitis is a disease characterized by inflammation of the gums that occurs in one or more areas without loss of attachments.1 in periodontitis, an inflammatory event that begins in the gum along with gingivitis spreads to the periodontal ligament, alveolar bone and soft tissues that support the tooth, causing the destruction of these structures.2 Cytokines are low molecular weight proteins that participate in the initial and active stages of inflammation and immunity. In periodontal disease pathogenesis, cytokine response has been reported to play a very critical role in determining disease progression.3 IL-1beta, MIP-1alfa and G-CSF are key cytokines in chronic inflammatory diseases and have the potential to initiate bone loss and tissue destruction seen in periodontal disease.4the purpose of this study; it is to determine the degree of inflammation and periodontal destruction by determining the levels of IL-1beta, MIP-1alfa, G-CSF cytokines in the gum groove fluid of periodontal healthy and diseased individuals.

Detailed description: Plaque index (PI), gingival index (GI), presence of bleeding on probing (BOP), pocket depth (PD) and clinical attachment level (CAL) were used After clinical evaluation, individuals were divided into 6 groups Healthy control groups, gingivitis groups, Stage I groups, Stage II groups, Stage III groups and Stage IV groups GCF samples were collected after clinical periodontal measurement. GCF was obtained using strips of filter paper Kolmogorov-Smirnov and Shapiro Wilk's tests used

Eligibility criteria: Inclusion Criteria:

* Clinical diagnosis of periodontal diseases
* Individuals must have 16 permanent teeth in their mouth

Exclusion Criteria:

* Diabetes
* İmmunological diseases

Primary outcomes:
measure: Measurement of IL-1Beta, G-CSF, MIP-1alfa levels in GCF
timeFrame: GCF samples were collected in the morning hours 24-48 hours after clinical periodontal measurement. GCF was obtained using strips of filter paper† from the buccal part of an interproximal region.
Secondary outcomes:
Other outcomes:
Locations:
city: Uşak
country: Turkey
facility: University of Usak
Study officials:
affiliation: University of Usak
name: ahu dikilitaş
role: PRINCIPAL_INVESTIGATOR
affiliation: University of Usak
name: fatih karaaslan
role: STUDY_CHAIR
affiliation: University of Usak
name: umut yiğit
role: STUDY_CHAIR
affiliation: University of Usak
name: esra özge aydın
role: STUDY_CHAIR
affiliation: University of Usak
name: Abdullah seçkin ertuğrul
role: STUDY_DIRECTOR
References: