Clinical trial NCT04596553: Effects of Essential and Non-Essential Amino Acids on Post-Exercise Muscle Collagen Synthesis in Young Men
Official title: The Effects of Essential and Non-Essential Amino Acids on Post-Exercise Muscle Collagen Synthesis in Young Men: a Randomized Controlled Trial
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Skeletal Muscle
Extracellular Matrix
Amino Acids Metabolism
Exercise
Dietary Supplements
Keywords: Muscle Collagen Turnover
Myofibrillar Protein Synthesis
Extracellular Matrix
Anabolism Signaling
Resistance Exercise
Skeletal Muscle
Bone Turnover
Lead sponsor: University of British Columbia
Sponsor class: OTHER
First posted: 2020-10-22
Enrollment: 17
Sex: MALE
Minimum age (years): 18
Maximum age (years): 35
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: PARALLEL
Intervention model description: - Double-blinded, randomized controlled, parallel design study, with 3 treatment groups (placebo, collagen peptide, and essential amino acid)
Primary purpose: BASIC_SCIENCE

Brief summary: The purpose of this study is to assess which amino acids are affecting acute muscle collagen synthesis in response to supplementation and exercise.

Detailed description: This is a double-blinded, randomized controlled, parallel design study, with 3 treatment groups (placebo, collagen peptide, and essential amino acid). The study will involve 3 visits: a baseline testing/familiarization session, and 2 exercise trials which will include muscle biopsies, blood draws, and the infusion of a tracer isotope in order to measure muscle protein synthesis.

Eligibility criteria: Inclusion Criteria:

* Can fluently read and write in English
* Male
* 18 - 35 years

Exclusion Criteria:

* Diabetes, cardiovascular disease, or current pharmaceutical therapy
* Medical conditions impacting ability to undertake strenuous physical activity
* Lower body resistance training in the previous 6 months
* BMI \> 30
* Currently smoking with cigarettes
* Allergy to any study supplements
* Currently taking any protein, amino acid, or collagen supplement
* Participate in greater than 2 hours of weekly vigorous exercise

Primary outcomes:
measure: Fractional synthetic rate (change over time) of Myofibrillar Protein Synthesis
timeFrame: 0 - 4 hours post-exercise
measure: Fractional synthetic rate (change over time) of Myofibrillar Protein Synthesis
timeFrame: 0 - 2 hours post-exercise
measure: Fractional synthetic rate (change over time) of Myofibrillar Protein Synthesis
timeFrame: 2 - 4 hours post-exercise
measure: Fractional synthetic rate (change over time) Muscle Collagen Synthesis
timeFrame: 0 - 4 hours post-exercise
measure: Fractional synthetic rate (change over time) Muscle Collagen Synthesis
timeFrame: 0 - 2 hours post-exercise
measure: Fractional synthetic rate (change over time) Muscle Collagen Synthesis
timeFrame: 2 - 4 hours post-exercise
measure: Pro-Collagen Type I N-Propeptide (PINP)
timeFrame: 1 hour prior to exercise
measure: Pro-Collagen Type I N-Propeptide (PINP)
timeFrame: 4 hours post exercise
Secondary outcomes:
measure: Plasma Amino Acids Response to Supplementation
timeFrame: 0 - 4 hours post-exercise
measure: Muscle mechanistic target of rapamycin Pathway Activation
timeFrame: 0 - 4 hours post-exercise
measure: PCR Gene Expression of Muscle Collagen Genes (Types I, III, IV)
timeFrame: 0 - 4 hours post-exercise
measure: Pro-Collagen Type III N-Propeptide (PIIINP)
timeFrame: 1 hour prior to exercise
measure: Pro-Collagen Type III N-Propeptide (PIIINP)
timeFrame: 4 hours post exercise
Other outcomes:
Locations:
city: Vancouver
country: Canada
facility: Chan Gunn Pavilion
state: British Columbia
Study officials:
affiliation: University of British Columbia
name: Cameron Mitchell, PhD
role: PRINCIPAL_INVESTIGATOR
References:
citation: Shaw G, Lee-Barthel A, Ross ML, Wang B, Baar K. Vitamin C-enriched gelatin supplementation before intermittent activity augments collagen synthesis. Am J Clin Nutr. 2017 Jan;105(1):136-143. doi: 10.3945/ajcn.116.138594. Epub 2016 Nov 16.
citation: Holm L, Rahbek SK, Farup J, Vendelbo MH, Vissing K. Contraction mode and whey protein intake affect the synthesis rate of intramuscular connective tissue. Muscle Nerve. 2017 Jan;55(1):128-130. doi: 10.1002/mus.25398. Epub 2016 Sep 26.
citation: Frontera WR, Ochala J. Skeletal muscle: a brief review of structure and function. Calcif Tissue Int. 2015 Mar;96(3):183-95. doi: 10.1007/s00223-014-9915-y. Epub 2014 Oct 8.
citation: Egerman MA, Glass DJ. Signaling pathways controlling skeletal muscle mass. Crit Rev Biochem Mol Biol. 2014 Jan-Feb;49(1):59-68. doi: 10.3109/10409238.2013.857291. Epub 2013 Nov 18.
citation: Holm L, van Hall G, Rose AJ, Miller BF, Doessing S, Richter EA, Kjaer M. Contraction intensity and feeding affect collagen and myofibrillar protein synthesis rates differently in human skeletal muscle. Am J Physiol Endocrinol Metab. 2010 Feb;298(2):E257-69. doi: 10.1152/ajpendo.00609.2009. Epub 2009 Nov 10.
citation: Alcock RD, Shaw GC, Tee N, Burke LM. Plasma Amino Acid Concentrations After the Ingestion of Dairy and Collagen Proteins, in Healthy Active Males. Front Nutr. 2019 Oct 15;6:163. doi: 10.3389/fnut.2019.00163. eCollection 2019.
citation: Miller BF, Olesen JL, Hansen M, Dossing S, Crameri RM, Welling RJ, Langberg H, Flyvbjerg A, Kjaer M, Babraj JA, Smith K, Rennie MJ. Coordinated collagen and muscle protein synthesis in human patella tendon and quadriceps muscle after exercise. J Physiol. 2005 Sep 15;567(Pt 3):1021-33. doi: 10.1113/jphysiol.2005.093690. Epub 2005 Jul 7.