Clinical trial NCT02668445: Molecular Response After Concurrent Exercise With Low Glycogen
Official title: The Effects of Glycogen Availability on the Skeletal Muscle Molecular Response After Concurrent Exercise
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Healthy
Keywords: Concurrent
muscle
glycogen
molecular
Lead sponsor: Wageningen University
Sponsor class: OTHER
First posted: 2016-01-29
Enrollment: 14
Sex: MALE
Minimum age (years): 18
Maximum age (years): 30
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: CROSSOVER
Primary purpose: TREATMENT

Brief summary: This is a randomized, counterbalanced crossover design, in which 14 male subjects will complete two experimental trials. Each trial will be separated by a minimum of at least 12 days (range: 12-30 days). The study aims to investigate the effects of low glycogen availability on skeletal muscle molecular response after a concurrent exercise day.

Detailed description: Two experimental exercise trials; a low carbohydrate (= LOW) and a high carbohydrate (= HIGH) trial. During both experimental days subjects will complete an continues submaximal cycling bout in the morning followed by a resistance exercise session in the early afternoon. During the LOW trial subjects will receive a low carbohydrate mixed meal, during the HIGH trial subjects will be provided with a high carbohydrate meal. In addition, on both experimental days subjects will receive adequate protein beverages. Lastly, protein will be provided post resistance exercise.

Eligibility criteria: Inclusion Criteria:

* Male gender between 18 and 30 years of age
* BMI between 18.5 - 25 kg/m2
* Participating in regular exercise training (at least 1 exercise session, and a maximum of 3 exercise session per week)
* Used to different types of exercise (endurance, resistance and/or concurrent)
* Willing to give blood samples
* Willing to give muscle biopsies
* No use of antibiotics during the study or in the past month
* No drug use
* Suitable veins for blood withdrawal
* Registered by a general-practitioner
* Consumption of alcohol beverages is less than 21 per week
* No blood donor during the study
* No vegetarian and willing to eat meat during the experimental days
* Able to be present and participate at both test days and pre testing moments
* Not employed, or intern, or working on thesis at the department of Human Nutrition at Wageningen University
* Not participating in another scientific study (except EetMeetWeet)
* Able to participate during the experimental days en pre tests
* Able to speak Dutch

Exclusion Criteria:

* Medical condition that can interfere with the study outcome (i.e. cardiovascular disease, pulmonary disease, gluten intolerance)
* Systolic blood pressure \>160 mmHg and/or diastolic blood pressure \>100 mmHg
* Use of medications known to interfere with gene expression in the muscles (i.e. statins, fenofibrate)
* Use of antithrombotic therapy (marcoumar, sintromitis).
* Diagnosed diabetes mellitus type 1 or 2
* (Chronic) injuries of the locomotor system that can interfere with the intervention

Primary outcomes:
measure: Proteins involved in hypertrophy
timeFrame: 10 hours
Secondary outcomes:
Other outcomes:
Locations:
city: Wageningen
country: Netherlands
facility: Division of Human Nutrition, Wageningen University
state: Gelderland
Study officials:
References: