Clinical trial NCT03747913: Oxidative Stress as an Acute Exercise-induced Mechanism of Stem and Progenitor Cell Mobilization
Official title: Effect of Oxidative Stress on Acute Exercise-induced Mobilization and Apoptosis of Hematopoietic, Endothelial and Mesenchymal Stem and Progenitor Cells and Mature Endothelial Cells in Healthy Young Male Subjects
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Healthy
Lead sponsor: Swiss Federal Institute of Technology
Sponsor class: OTHER
First posted: 2018-11-20
Enrollment: 18
Sex: MALE
Minimum age (years): 18
Maximum age (years): 35
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: NA
Intervention model: SINGLE_GROUP
Intervention model description: Each participant enrolled in the study will be part of the supplementation and control condition group in a non-randomized order
Primary purpose: BASIC_SCIENCE

Brief summary: It is already known from literature that exercise mobilizes stem and progenitor cells into the peripheral blood. However, the exact mechanisms thereof remain to be fully elucidated.

The investigators hypothesize that exercise-induced oxidative stress could be at least one of the responsible mechanisms and therefore want to study the exercise-induced stem and progenitor cell mobilization in a group of healthy young men when they exercise with, compared to when they exercise without antioxidative supplementation.

The primary outcome is numbers of stem and progenitor cells in the peripheral blood after an acute bout of exercise. As a secondary outcome, numbers of apoptotic mature and immature cells in the blood will be analysed.


Eligibility criteria: Inclusion Criteria:

* Non-smoking
* Healthy
* Normal Body-Mass-Index (BMI): 18.5-24.9 kg∙m-2
* Active (fulfill World Health Organization-guidelines for recommended levels of physical activity for adults aged 18 - 64 years)
* Willing to adhere to the study rules

Exclusion Criteria:

* Known or suspected non-compliance, drug or alcohol abuse
* Inability to follow the procedures of the study, e.g. due to language problems, psychological disorders etc. of the participant
* Previous enrolment into the current study
* Enrolment of the investigator, his/her family members, employees and other dependent persons
* Regular intake of medication
* Regular intake of antioxidant supplementations

Primary outcomes:
measure: Changes in exercise-dependently mobilized stem and progenitor cell numbers in the peripheral blood with antioxidative supplementation
timeFrame: On visit 2 after the exercise test without supplementation and then again on visit 3 after the exercise test with prior supplementation (1 week)
Secondary outcomes:
measure: Changes in numbers of exercise-induced apoptotic cells and oxidative stress markers in the peripheral blood with antioxidative supplementation
timeFrame: On visit 2 after the exercise test without supplementation and then again on visit 3 after the exercise test with prior supplementation (1 week)
Other outcomes:
Locations:
city: Zürich
country: Switzerland
facility: Exercise Physiology Lab, Institute of Human Movement Sciences and Sport, ETH Zurich
state: Zurich
Study officials:
References:
citation: Schmid M, Martins HC, Schratt G, Kropfl JM, Spengler CM. MiRNA126 - RGS16 - CXCL12 Cascade as a Potential Mechanism of Acute Exercise-Induced Precursor Cell Mobilization. Front Physiol. 2021 Dec 9;12:780666. doi: 10.3389/fphys.2021.780666. eCollection 2021.