Clinical trial NCT05365334: Skeletal Muscle and Adipose Tissue Responses After Acute Exercise: Comparing Effects of Three Different Intensities
Official title: Skeletal Muscle and Adipose Tissue Responses After Acute Exercise: Comparing Effects of Three Different Intensities
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Cellular Responses Within Skeletal Muscle in Response to Exercise of Different Intensities
Relationship Between the Cellular Responses in Skeletal Muscle and the Changes in the Metabolomic Profile
Changes Within Adipose Tissue in Response to Exercise at the Three Different Intensities
Lead sponsor: University of Michigan
Sponsor class: OTHER
First posted: 2022-05-09
Enrollment: 45
Sex: ALL
Minimum age (years): 18
Maximum age (years): 40
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: PARALLEL
Primary purpose: BASIC_SCIENCE

Brief summary: The study will assess the exercise-induced changes in chromatin accessibility and gene expression in a cell type-specific manner in skeletal muscle from healthy adults in response to exercise at three different intensities - 1) low-intensity continuous exercise, 2) moderate-intensity continuous exercise, and 3) high-intensity interval exercise.


Eligibility criteria: Inclusion Criteria:

* Age:18-40
* Body Mass Index: 20-30 kg/m2
* Must engage in regularly planned aerobic-type exercise at moderate-vigorous intensity, 3 times per week for at least 30-60 minutes/session
* Women must have regularly occurring menses and must be premenopausal
* Subject willingness to opt-in for the Michigan Genomics Initiative (MGI) Repository

Exclusion Criteria:

* Evidence/history of cardiovascular or metabolic disease.
* Taking anti-coagulate medications or medications known to affect metabolism or inflammation
* Weight instability ≥ ±3kg in the last 6 months
* Tobacco or e-cigarette users
* Women must not be pregnant or actively lactating

Primary outcomes:
measure: post-translational modifications of skeletal muscle oxidative enzymes
timeFrame: 30 minutes before exercise
measure: post-translational modifications of skeletal muscle oxidative enzymes
timeFrame: immediately after exercise
measure: post-translational modifications of skeletal muscle oxidative enzymes
timeFrame: 60 minutes after exercise
measure: post-translational modifications of Adipose tissue metabolic proteins
timeFrame: 15 minutes before exercise
measure: post-translational modifications of Adipose tissue metabolic proteins
timeFrame: 75 minutes after exercise
measure: mRNA expression of skeletal muscle oxidative enzymes
timeFrame: 30 minutes before exercise
measure: mRNA expression of skeletal muscle oxidative enzymes
timeFrame: immediately after exercise
measure: mRNA expression of skeletal muscle oxidative enzymes
timeFrame: 60 minutes after exercise
measure: mRNA expression of adipose tissue metabolic enzymes
timeFrame: 15 minutes before exercise
measure: mRNA expression of adipose tissue metabolic enzymes
timeFrame: 75 minutes after exercise
Secondary outcomes:
Other outcomes:
Locations:
city: Ann Arbor
country: United States
facility: University of Michigan
state: Michigan
Study officials:
References: