Clinical trial NCT05475951: Gasdermin-D and Pannexin-1 in Pregnancy
Official title: Investigation of Gasdermin-D and Pannexin-1 in Fat and Placental Tissues in Pregnant Women With Obesity
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Pregnancy Complications
Maternal Obesity
Keywords: gasdermin-D
pannexin-1
placenta
omental tissue
fat tissue
maternal obesity
inflammation
oxidative stress
biochemistry
ELISA
Lead sponsor: Haseki Training and Research Hospital
Sponsor class: OTHER
First posted: 2022-07-27
Enrollment: 79
Sex: FEMALE
Minimum age (years): 20
Maximum age (years): 35
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: NON_RANDOMIZED
Intervention model: PARALLEL
Primary purpose: BASIC_SCIENCE

Brief summary: One -third of fertile women around the world are overweight or obese. This means increasing risk for both the mother and the child. Fat tissue is a factor in gestational DM development and the increase in material inflammation and oxidative stress. According to new knowledge, it is an important need to examine molecules that are not handled in new and human research in these mechanisms in fat and placenta tissues in obesity. For this purpose, the examination of the expression of gasdermin-D and pannex-1 proteins, which are shown to be produced in the cells of both tissues, is worth investigating in human fat tissue and placenta. Gasdermins and pannexins are proteins intersecting by interacting in cellular functions.

Gasdermins cause piroptosis, a type of litic proinflammatory cell death. Pannexin-1 plays in various cellular functions, including inflammation. These are examined in a small number of in vitro studies in material fat tissue and placenta, and the design of this study in people whose applications are applied is original in humans.

The status of the expressions of the gasdermin-D and pannexin-1 proteins, which will be examined for the first time in obese pregnant women's fat and placental tissues, are the subject of this research with each other and their relationship with other maternal and neonatal data.


Eligibility criteria: Inclusion Criteria:

* Being in the 20-35 age range
* Term pregnancy (37-41 weeks)
* Healthy pregnancy follow-up
* Planned cesarean section
* Having no complication in cesarean delivery
* Being a placenta with front or rear walls

Exclusion Criteria:

* Intrauterine surgical procedure
* Presence of prenatal placental structural disorder
* Multiple pregnancies

Primary outcomes:
measure: Prediction of macrosomy
timeFrame: 7 months
Secondary outcomes:
Other outcomes:
Locations:
city: Sultangazi
country: Turkey
facility: Haseki Training and Research Hospital
state: İstanbul
Study officials:
affiliation: Departmant of Obstetrics and Gynecology in Hasek, Training and Research Hospital, Istanbul, Turkey
name: Ali Cetin, Professor
role: STUDY_DIRECTOR
References:
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