Clinical trial NCT03975777: Time-course of Mitochondrial Biogenic Gene and Protein Expression in Exercised Human Skeletal Muscle
Official title: Impact of Exercise Intensity on the Time-course of Mitochondrial Biogenic Gene and Protein Expression in Human Skeletal Muscle.
Study type: INTERVENTIONAL
Overall status: UNKNOWN
Phase: NA
Conditions: Mitochondrial Biogenesis
Lead sponsor: Queen's University
Sponsor class: OTHER
First posted: 2019-06-05
Enrollment: 10
Sex: MALE
Minimum age (years): 18
Maximum age (years): 30
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: CROSSOVER
Primary purpose: BASIC_SCIENCE

Brief summary: This study examines the impact of exercise intensity on the 12-hour time-course of mitochondrial biogenic gene and protein expression in human skeletal muscle. Briefly, participants will perform two experimental sessions involving an acute bout of work-matched low- or high-intensity interval cycling exercise in a randomized crossover fashion, two weeks apart. Skeletal muscle biopsies will be obtained from the vastus lateralis (3 from each leg) before and 1, 3, 6, 9, and 12 hours post-exercise. Changes in the expression of various transcription factors and mitochondrial proteins will be examined at the mRNA and protein level to determine the time-course of changes in these factors differs between exercise protocols of different intensities.


Eligibility criteria: Inclusion Criteria:

* recreationally active (no more than three hours of structured weekly physical activity)
* not currently involved in a systematic training program for improving cardiorespiratory fitness, muscular strength, and/or sport-specific skills
* body mass index \< 30 kg/m2

Exclusion Criteria:

* presence of cardiometabolic disease
* taking regular prescription medication for the treatment of cardiometabolic disease
* current cigarette smoker

Primary outcomes:
measure: Change in peroxisome proliferator activated receptor (PPAR) gamma co-activator 1-alpha (PGC-1α) messenger ribonucleic acid (mRNA) expression
timeFrame: before exercise to 1, 3, 6, 9, and 12 hours post-exercise
Secondary outcomes:
measure: Change in PGC-1α protein content
timeFrame: before exercise to 1, 3, 6, 9, and 12 hours post-exercise
measure: Change in nuclear factor-erythroid 2 like 2 (Nrf2) mRNA expression
timeFrame: before exercise to 1, 3, 6, 9, and 12 hours post-exercise
measure: Change in Nrf2 protein content
timeFrame: before exercise to 1, 3, 6, 9, and 12 hours post-exercise
measure: Change in PPAR-beta mRNA expression
timeFrame: before exercise to 1, 3, 6, 9, and 12 hours post-exercise
measure: Change in estrogen related receptor (ERR)-gamma mRNA expression
timeFrame: before exercise and 1, 3, 6, 9, and 12 hours post-exercise
measure: Change in leucine-rich pentatricopeptide repeat containing protein 130 (LRP130) mRNA expression
timeFrame: before exercise to 1, 3, 6, 9, and 12 hours post-exercise
measure: Change in LRP130 protein content
timeFrame: before exercise and 1, 3, 6, 9, and 12 hours post-exercise
measure: Change in nuclear respiratory factor-1 (NRF-1) mRNA expression
timeFrame: before exercise to 1, 3, 6, 9, and 12 hours post-exercise
measure: Change in NRF-1 protein content
timeFrame: before exercise to 1, 3, 6, 9, and 12 hours post-exercise
measure: Change in mitochondrial transcription factor A (TFAM) mRNA expression
timeFrame: before exercise and 1, 3, 6, 9, and 12 hours post-exercise
measure: Change in TFAM protein content
timeFrame: before exercise and 1, 3, 6, 9, and 12 hours post-exercise
measure: Change in p53 mRNA expression
timeFrame: before exercise to 1, 3, 6, 9, and 12 hours post-exercise
measure: Change in cytochrome c oxidase subunit 4 (COXIV) mRNA expression.
timeFrame: before exercise to 1, 3, 6, 9, and 12 hours post-exercise
measure: Change in COXI mRNA expression
timeFrame: before exercise to 1, 3, 6, 9, and 12 hours post-exercise
measure: Change in COXII mRNA expression
timeFrame: before exercise to 1, 3, 6, 9, and 12 hours post-exercise
measure: Change in nicotinamide adenine dinucleotide (NADH) subunit 1 (ND1) mRNA expression
timeFrame: before exercise and 1, 3, 6, 9, and 12 hours post-exercise
measure: Change in ND4 mRNA expression
timeFrame: before exercise to 1, 3, 6, 9, and 12 hours post-exercise
measure: Change in citrate synthase (CS) mRNA expression
timeFrame: before exercise to 1, 3, 6, 9, and 12 hours post-exercise
measure: Change in NRF-2 mRNA expression
timeFrame: before exercise and 1, 3, 6, 9, and 12 hours post-exercise
measure: Change in mitochondrial transcription factor B1 (TFB1M) mRNA expression
timeFrame: before exercise to 1, 3, 6, 9, and 12 hours post-exercise
measure: Change in mitochondrial transcription factor B2 (TFB2M) mRNA expression
timeFrame: before exercise and 1, 3, 6, 9, and 12 hours post-exercise
Other outcomes:
Locations:
city: Kingston
country: Canada
facility: School of Kinesiology and Health Studies
state: Ontario
Study officials:
affiliation: School of Kinesiology and Health Studies
name: Brendon J. Gurd, PhD
role: PRINCIPAL_INVESTIGATOR
References: