Clinical trial NCT03991117: Lower and Higher Load Resistance Exercise Protocols: Acute Muscle Activation and Skeletal Muscle Hypertrophy
Official title: Muscle Fibre Activation is Unaffected by Load and Repetition Duration When Resistance Exercise is Performed to Task Failure
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Muscle Hypertrophy
Muscle Fibre Activation
Keywords: muscle fibre activation
electromyography
Lead sponsor: McMaster University
Sponsor class: OTHER
First posted: 2019-06-19
Enrollment: 10
Sex: MALE
Minimum age (years): 18
Maximum age (years): 30
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: CROSSOVER
Intervention model description: Each individual performed four bouts of resistance exercise that varied in load and speed of contraction on two separate days (one condition each leg per visit).
Primary purpose: BASIC_SCIENCE

Brief summary: How much weight an individual lifts per workout does not dictate the relative increase in muscle size the individual gains following weeks of training, which is contrary to current strength training dogma. Specifically, researchers have concluded that so long as an individual performs resistance exercise with maximum effort, it is not necessary to lift with relatively heavy loads. However, other laboratories, on the basis of surface electromyography measurements, have challenged the thesis that lighter loads can result in the hypertrophy of larger, type II muscle fibres. Therefore, the purpose of this study was to combine surface electromyography measurements with direct measurements of muscle fibre activation to see if muscle fibre activation was truly dependent on load. The investigators hypothesized that all muscle fibres would be activated when the resistance exercise was performed with maximal effort.

Detailed description: PROCEDURES INVOLVED IN THE RESEARCH

The total time commitment of this study will be \~7 hours. Each participant's first visit (familiarization) will include an interview through where the researchers will determine if there are any reasons that the participant cannot take part in the study. The researchers will explain the study protocol and, upon the participant's consent, the investigators will obtain the participant's knee extension maximum voluntary contraction (MVC) on a dynamometer (Biodex, Shirley, USA) and a 1 repetition-maximum (RM) test on a knee extension machine. Following that, the investigators will determine the next two dates (trial days) in which the participant will come in.

The participant will be asked to come in fasted on two separate trial days, each separated by two weeks, after the participant's familiarization visit. The participant will be required to avoid physical activity 48 hours before each of the three visits. There are four conditions in this study, each varying in relative load and speed of contraction. The participant will complete two conditions on each trial day, one with each leg. The conditions include: 80%RM Normal (80N; 1 eccentric: 1 pause: 1 concentric), 80% Slow (80S; 3:1:3), 30% Normal (30N; 1:1:1) and 30% Slow (30S; 3:1:3). Each condition will include three sets to voluntary failure on a knee extension machine. Before and after each set the participant will perform three MVCs on the dynamometer identical to what the participant did on the familiarization day. The investigators will record the participant's rating of perceived exertion and effort via the Borg scale and electromyography (EMG), respectively. The participant will have one biopsy taken pre-training and two, one per condition/leg, one hour post-training.

The REB Project Number for this study is: 802

Eligibility criteria: Inclusion Criteria:

* healthy
* male
* 18-30 years of age

Exclusion Criteria:

* Have any acute or chronic illness such as cardiac, pulmonary, liver, or kidney abnormalities, uncontrolled hypertension, insulin- or non-insulin dependent diabetes or other metabolic disorders-all ascertained through a medical history screening questionnaire
* Experience arthritic conditions
* Smoke tobacco products
* Consume any analgesic or anti-inflammatory drug(s), prescription or non-prescription, chronically
* Have a history of neuromuscular complications
* Are on any medications known to affect protein metabolism (i.e. corticosteroids, non-steroidal anti-inflammatories, or prescription strength acne medications).
* Have an extensive history of RE training in the year prior to study entry.

Primary outcomes:
measure: Concentration of glycogen in muscle fibres
timeFrame: three weeks
measure: Amplitude of electrical activity above the quadriceps muscles
timeFrame: three weeks
Secondary outcomes:
Other outcomes:
Locations:
Study officials:
affiliation: McMaster University
name: Stuart Phillips, PhD
role: PRINCIPAL_INVESTIGATOR
References: