Clinical trial NCT06236659: Acute Resistance Exercise and Hydrolyzed Collagen Supplementation
Official title: The Dose-response of Vitamin C-enriched Collagen on Markers of Collagen Synthesis in Middle-Aged Men and Women Following Resistance Exercise
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Diet
Food
Nutrition
Keywords: Hydrolyzed collagen
Resistance Exercise
Collagen Synthesis
Proline
Glycine
Lead sponsor: Liverpool John Moores University
Sponsor class: OTHER
Collaborators: name: University of East Anglia
First posted: 2024-02-01
Enrollment: 10
Sex: ALL
Minimum age (years): 40
Maximum age (years): 65
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: CROSSOVER
Intervention model description: Double-blind, randomized cross-over design
Primary purpose: BASIC_SCIENCE

Brief summary: This study aimed to investigate the effect of different doses of hydrolyzed collagen (HC) with resistance exercise (RE) on whole body collagen synthesis in middle-aged males and females.

Detailed description: This study aimed to investigate the effect of different doses of hydrolyzed collagen (HC) with resistance exercise (RE) on whole body collagen synthesis in healthy middle-aged males and females.

Healthy middle-aged males and females ingested 0 grams, 15 grams or 30 grams HC with 50 milligrams vitamin C 1h prior to performing four sets' leg press RE at 10-repetition maximum load, after which they rested for six hours. Blood samples were collected throughout each trial to analyse procollagen type Ⅰ N-terminal propeptide (PⅠNP, a biomarker of collagen synthesis) and β-isomerized C-terminal telopeptide of type I collagen (β-CTX, a biomarker of collagen breakdown) concentration, and the concentration of 18 amino acids that constitute collagen.

This is the first study to investigate the combined effect of different doses HC with high-intensity RE on whole body collagen synthesis in middle-aged men and women. If 30 grams HC intake with RE does augment collagen synthesis more than RE alone, this suggests that long-term HC intake with chronic RE would be beneficial for tendon health, because collagen is the most abundant protein in tendon.

Eligibility criteria: Inclusion Criteria:

* At least 12 months experienced in resistance exercise (including weekly lower body exercise)
* Free from musculoskeletal injury

Exclusion Criteria:

* Having a history of patellar tendon pathology
* Vegan
* Consumed nutritional supplements or medication purported to have beneficial effects on muscle-tendon properties (e.g. antioxidants, protein, etc.)
* Had a sustained a lower limb injury in the previous six months
* Smoker/vaper
* Having reached menopause (only for middle-aged female participants)

Primary outcomes:
measure: Change in a marker of collagen synthesis
timeFrame: At rest immediately prior to HC ingestion, 0.5-hour post RE, 1-hour post RE, 2-hour post RE, 4-hour post RE and 6-hour post RE
measure: Change in a marker of collagen breakdown
timeFrame: At rest, immediately prior to HC ingestion, 0.5-hour post RE, 2-hour post RE and 6-hour post RE
Secondary outcomes:
measure: Estrogen concentrations in women in each trial
timeFrame: At rest immediately prior to HC ingestion
measure: Changes in amino acids concentrations in blood
timeFrame: At rest immediately prior to HC ingestion, 0.5-hour post HC ingestion, 1-hour post HC ingestion, 0.5-hour post RE, 1-hour post RE, 2-hour post RE, 4-hour post RE and 6-hour post RE
Other outcomes:
Locations:
city: Liverpool
country: United Kingdom
facility: Dr Rob Erskine
Study officials:
affiliation: Liverpool John Moores University
name: Robert M Erskine
role: PRINCIPAL_INVESTIGATOR
References: