Clinical trial NCT03563118: Evaluation of Prolidase in Obstructive Sleep Apnea Syndrome
Official title: Evaluation of Prolidase as an Oxidative Stress Marker in Obstructive Sleep Apnea Syndrome
Study type: OBSERVATIONAL
Overall status: UNKNOWN
Conditions: Obstructive Sleep-Apnea Syndrome
Keywords: Prolidase Activity
Obstructive Sleep-Apnea Syndrome
Lead sponsor: Sakarya University
Sponsor class: OTHER
First posted: 2018-06-20
Enrollment: 74
Sex: ALL
Minimum age (years): 18
Maximum age (years): 65
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: no
Observational model: OTHER
Time perspective: PROSPECTIVE
Sampling method: NON_PROBABILITY_SAMPLE
Study population: Patient (male/female: 43/31) were diagnosed with OSA after night polysomnography (PSG)

Brief summary: Obstructive sleep apnea (OSA) is a highly prevalent breathing disorder in sleep. We have aimed to evaluate the relationship between OSA and prolidase activity, the oxidative stress index (OSI), total antioxidative capacity (TAC), and total oxidative capacity (TOC) and the relationship between carotid intima media thickness (CIMT).

Detailed description: This study was approved by the local ethics committee in accordance with the Helsinki Declaration. Written informed consent was received from the OSAS Subjects patients and control subjects before enrolment in the study. The patient and control cohorts were recruited from the Pulmonary Medicine Department, Medical Faculty, Yuzuncu Yıl University. Blood samples were analysed at the Biochemistry Laboratory of Harran University Medical Faculty TAC and TOS levels were measured by using an automated measurement method. TAC measurement method involves the production of a potent biological hydroxyl radical. Ferrous ion solution is mixed with hydrogen peroxide. Thus, it is possible to measure the anti-oxidative effect of the sample against the potent free radical reactions initiated by the production of the hydroxyl radical. TOS method is based on the oxidation of ferrous ion to ferric ion in the presence of various oxidant species in acidic medium and the measurement of the ferric ion by xylenol orange. mmol Trolox equivalent (equiv)/L, mmol H2O2 /L and mg/dL, respectively.

Eligibility criteria: Inclusion Criteria:

* Patients were diagnosed with OSA after night polysomnography (PSG).
* Patients were enrolled in the study following receipt of their written informed consent.

Exclusion Criteria:

* Patients had ischaemic cardiovascular diseases,
* Patients had chronic obstructive pulmonary diseases,
* Patients had ischaemic cerebral diseases,
* Patients had chronic inflammatory diseases,
* Patients had chronic and acute systemic infections.

Primary outcomes:
measure: Prolidase activity
timeFrame: 1 month
Secondary outcomes:
Other outcomes:
Locations:
city: Sakarya
country: Turkey
facility: Sakarya University Research and Training Hospital
state: Merkez
city: Sakarya
country: Turkey
facility: Sakarya University Research and Training Hospital
Study officials:
affiliation: Sakarya University Research and Training Hospital
name: Havva Sayhan
role: STUDY_CHAIR
References:
citation: Yamauchi M, Nakano H, Maekawa J, Okamoto Y, Ohnishi Y, Suzuki T, Kimura H. Oxidative stress in obstructive sleep apnea. Chest. 2005 May;127(5):1674-9. doi: 10.1378/chest.127.5.1674.