Clinical trial NCT04232254: Defining Beef and Meal Frequency as Key Components of a Healthy Eating Pattern for Muscle Health and Wellbeing
Official title: Defining Beef and Meal Frequency as Key Components of a Healthy Eating Pattern for Muscle Health and Wellbeing
Study type: INTERVENTIONAL
Overall status: UNKNOWN
Phase: NA
Conditions: Meal Frequency
Dietary Habits
Muscle Protein Synthesis
Lead sponsor: University of Illinois at Urbana-Champaign
Sponsor class: OTHER
Collaborators: name: National Cattlemen's Beef Association
First posted: 2020-01-18
Enrollment: 48
Sex: ALL
Minimum age (years): 20
Maximum age (years): 40
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: FACTORIAL
Primary purpose: TREATMENT

Brief summary: Adopting a healthy eating pattern is important for meeting dietary recommendations and weight management. Although less clear, it seems reasonable to assume that the eating patterns we typically follow can also affect our psychological wellbeing. As such, healthy eating patterns are often adapted to suit one's personal preference. For example, many people choose to follow a vegetarian-style eating pattern whereby meat, poultry, and seafood are excluded from the diet. However, current research suggests that vegetarian eating patterns may result in decreased synthesis of new muscle proteins when compared to the typical meat-based US-style diet. This ultimately leads to reduced muscle quality and mass which increases the risk of dependence and mobility limitations later in life.

Another important factor to consider when adapting a healthy eating pattern is the frequency and distribution of meals throughout the day. In the US, protein intake is typically skewed throughout the day such that people consume more protein at dinner when compared to breakfast. This skewed distribution combined with a low meal frequency (3 meals per day) can also sacrifice the rate of muscle protein synthesis. As such, it is important to investigate the interaction between food choices, meal frequency, and protein distribution to promote muscle health and prevent development of disease and disability. In addition, it is also important to understand how these eating patterns affect enjoyment and pleasure following meals. This work will help to determine healthy eating patterns that promote muscle health and psychological wellbeing.


Eligibility criteria: Inclusion Criteria:

* Age 20-40
* Pre-menopausal
* Recreationally active
* Weight stable for prior 6 months
* Consumption of meat-based diet for \>6 months

Exclusion Criteria:

* Age outside of range (20 - 40)
* Pregnancy
* Irregular menstrual cycles
* Participation in previous research using 2H2O or \[13C6\]phenylalanine
* Participation in other ongoing research that interferes with this study (e.g., conflicting diet, activity interventions, etc.)
* Any hospitalization or surgery for a metabolic, cardiovascular, or neuromusculoskeletal complication within the past year
* Allergy or hypersensitivity to local anesthetics, latex, or adhesives (bandages, medical tape, etc.)
* Excess scarring after injury
* History of excess bleeding after cut
* Chronic or frequent dizziness/fainting, and arm or leg weakness/numbness
* Arthritis
* Tumors
* Mental Illness
* Hepatorenal, cardiovascular musculoskeletal, autoimmune, or neurological disease or disorder
* Predisposition to hypertrophic scarring or keloid formation
* Physical activity limitations
* Consumption of ergogenic-levels of dietary supplements that may affect muscle mass (e.g., creatine, HMB), insulin-like substances, or anabolic/catabolic pro-hormones (e.g., DHEA) within 6 weeks prior to participation
* Consumption of thyroid, androgenic, or other medications known to affect endocrine function
* Consumption of medications known to affect protein metabolism (e.g., prescription-strength corticosteroids, nonsteroidal anti-inflammatories, or acne medication)
* Unwillingness to comply with study procedures
* Weight unstable (variation \>5% of bodyweight in last 6-12 months)
* Pregnancy
* Allergy to dairy product or lactose intolerance
* Current or previous tobacco use with last 6 months
* Obesity (body mass index; BMI \> 30 kg m-2)
* Score of less than 14 or greater than 24 on Godin-Shephard Leisure-Time Physical Activity Questionnaire

Primary outcomes:
measure: Compare fractional synthesis rate of myofibrillar proteins to different dietary protein food sources and meal frequencies
timeFrame: Day 1 - Day 9
measure: Compare fractional synthesis rate of myofibrillar proteins to different dietary protein food
timeFrame: During the 3-hour post-absorptive period prior to ingestion of a mixed meal containing either plant- or animal based protein and throughout the 5-hour post-prandial period following meal ingestion
Secondary outcomes:
measure: Valence (Pleasantness or Unpleasantness)
timeFrame: Immediately prior to workout, immediately post workout, and immediately post meal on habituation days 2, 4, and 6 and intervention days 3, 5, and 7
measure: Arousal (Alertness)
timeFrame: Immediately prior to workout, immediately post workout, and immediately post meal on habituation days 2, 4, and 6 and intervention days 3, 5, and 7
measure: Arousal (Alertness)
timeFrame: Immediately prior to workout, immediately post workout, and immediately post meal on habituation days 2, 4, and 6 and intervention days 3, 5, and 7
measure: Plasma Amino Acid Concentration
timeFrame: During the 3-hour post-absorptive period prior to ingestion of a mixed meal containing either plant- or animal based protein and throughout the 5-hour post-prandial period following meal ingestion
measure: Plasma Insulin Concentration
timeFrame: During the 3-hour post-absorptive period prior to ingestion of a mixed meal containing either plant- or animal based protein and throughout the 5-hour post-prandial period following meal ingestion
measure: Anabolic Signaling
timeFrame: Prior to, 2 hours, and 5 hours following ingestion of a mixed meal containing either plant- or animal based protein
measure: Whole-body Leucine Oxidation
timeFrame: During the 3-hour post-absorptive period prior to ingestion of a mixed meal containing either plant- or animal based protein and throughout the 5-hour post-prandial period following meal ingestion
Other outcomes:
Locations:
city: Urbana
country: United States
facility: Freer Hall
state: Illinois
Study officials:
affiliation: University of Illinois at Urbana-Champaign
name: Nicholas A Burd, PhD
role: PRINCIPAL_INVESTIGATOR
References: