Clinical trial NCT03303729: Influence of Carbohydrate on Amino Acid Absorption From Dietary Protein (ICADP)
Official title: Influence of Carbohydrate on Amino Acid Absorption From Dietary Protein
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Muscle Protein Synthesis
Amino Acid
Keywords: Carbohydrate
Cluster Dextrin
Exercise training
Protein supplement
Lead sponsor: Bispebjerg Hospital
Sponsor class: OTHER
Collaborators: name: University of Birmingham
First posted: 2017-10-06
Enrollment: 10
Sex: MALE
Minimum age (years): 18
Maximum age (years): 30
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: CROSSOVER
Primary purpose: BASIC_SCIENCE

Brief summary: 10 young males will be recruited to participate in a randomized double blinded crossover study to investigate the influence of concomitant intake of two different types of carbohydrate with protein hydrolysate on the absorption kinetics of protein-derived amino acids and the impact on myofibrillar protein synthesis. During the trial days the subjects will perform a bout of whole body resistance exercise and ingest a supplement of meat hydrolysate labeled with D5-phenylalanine together with randomized and double blinded type of carbohydrate consisting of either glucose or cluster dextrin.

The primary outcome is the time to obtain peak concentration of D5-phenylalanine after intake of meat hydrolysate with either glucose or cluster dextrin.

Hypotheses:

I) The cluster dextrin will increase the absorption of the amino acids from the meat hydrolysate, and thereby result in a faster rise and a higher peak in plasma amino acid concentration in the postprandial period.

II) The meat hydrolysate will increase the protein synthesis rate to a higher extent when combined with cluster dextrin than with glucose.


Eligibility criteria: Inclusion Criteria:

* young healthy adults,
* organised sport/training 1-3 times per week

Exclusion Criteria:

* Subject younger than 18 years or above 30 years
* BMI \> 30
* Chronic medical diseases: active cancer, renal diseases, metabolic diseases like diabetes mellitus.
* Physical inactivity. Subjects must participate in systematized weekly exercise 1-3 times per week.
* Participating in systematized exercise more than 3 times per week.
* Vegetarian.
* Participating in a special diet which deviates from the standard of young adults.

Primary outcomes:
measure: Protein hydrolysate amino acid label time to peak appearance in plasma
timeFrame: From hour 0 to hour 3
Secondary outcomes:
measure: Myofibrillar protein fractional synthesis rate response to protein and carbohydrate intake after a whole body resistance exercise
timeFrame: from hour 0,5 to hour hour 1. From hour 1 to hour 3. From hour 0,5 to hour 3.
measure: Protein hydrolysate amino acid label area under the concentration curve in plasma
timeFrame: From hour 0 to hour 3
measure: Insulin concentration
timeFrame: From hour 0 to hour 3
Other outcomes:
Locations:
city: Copenhagen
country: Denmark
facility: Bispebjerg Hospital
state: Copenhagen NV
Study officials:
affiliation: Bispebjerg Hospital
name: Jacob L. Bülow, MD
role: PRINCIPAL_INVESTIGATOR
affiliation: University of Birmingham
name: Lars Holm, Prof.
role: PRINCIPAL_INVESTIGATOR
affiliation: Bispebjerg Hospital
name: Michael Kjaer, MD, prof.
role: PRINCIPAL_INVESTIGATOR
affiliation: University of Copenhagen
name: Sofus Sølyst, stud.med.
role: PRINCIPAL_INVESTIGATOR
affiliation: University of Copenhagen
name: Mikkel Jensen, Stud.scient.
role: PRINCIPAL_INVESTIGATOR
References:
citation: Nishimura Y, Jensen M, Bulow J, Thomsen TT, Arimitsu T, van Hall G, Fujita S, Holm L. Co-ingestion of cluster dextrin carbohydrate does not increase exogenous protein-derived amino acid release or myofibrillar protein synthesis following a whole-body resistance exercise in moderately trained younger males: a double-blinded randomized controlled crossover trial. Eur J Nutr. 2022 Aug;61(5):2475-2491. doi: 10.1007/s00394-021-02782-y. Epub 2022 Feb 19.