Clinical trial NCT01265953: Chemoprevention of Prostate Cancer, HDAC Inhibition and DNA Methylation
Official title: Chemoprevention of Prostate Cancer, HDAC Inhibition and DNA Methylation
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Prostate Cancer Prevention
Keywords: cancer prevention
isothiocyanate
sulforaphane
Lead sponsor: Portland VA Medical Center
Sponsor class: FED
Collaborators: name: National Cancer Institute (NCI)
name: Oregon State University
name: OHSU Knight Cancer Institute
First posted: 2010-12-23
Enrollment: 98
Sex: MALE
Minimum age (years): 21
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: PARALLEL
Primary purpose: PREVENTION

Brief summary: The objective of the study is to identify mechanisms by which compounds found in cruciferous vegetables alter gene expression via epigenetic modifications (changes in gene expression) and may prevent prostate cancer development.

The investigators have found that sulforaphane (SFN), an isothiocyanate found in cruciferous vegetables, inhibits histone deacetylase (HDAC) activity in human colorectal and prostate cancer cells.

Detailed description: Prostate cancer is the most frequently diagnosed non-cutaneous cancer and is the second leading cause of cancer death in American men. The precise etiologic factors that initiate and enhance the progression of prostate cancer remain unknown, but epigenetic alterations and diet/lifestyle factors have come forth as significant contributing factors. Epidemiologic studies suggest that cruciferous vegetable intake decreases the risk for prostate cancer. The long-term goal of this proposal is to identify mechanisms by which dietary compounds, such as those found in cruciferous vegetables decrease prostate cancer risk. The objective of the study is to identify mechanisms by which compounds found in cruciferous vegetables alter gene expression via epigenetic modifications and may prevent prostate cancer development.

The investigators have found that SFN, an isothiocyanate found in cruciferous vegetables, inhibits HDAC activity in human colorectal and prostate cancer cells.

Targeting the epigenome, including the use of HDAC and DNA methyltransferase (DNMT) inhibitors, is an evolving strategy for cancer chemoprevention and both have shown promise in cancer clinical trials.

This Randomized, Double Blind, Clinical Trial will address the following objectives:

1. Identify distribution of SFN and its metabolites and HDAC inhibition following supplementation with an SFN-rich broccoli sprout extract in subjects at risk for prostate cancer (Primary Endpoints)
2. Investigate the effects of supplementation with an SFN-rich broccoli sprout extract on DNA methylation status and proliferation markers in a pre-biopsy setting (secondary analysis)

The effects of short-term supplementation with an SFN-rich broccoli sprout extract on benign epithelial tissue will be studied in men characterized as being at risk for prostate cancer in a randomized, placebo-controlled trial. Men scheduled for prostate biopsy will be recruited into the trial.

Following successful completion of the consent, two 10 mL blood specimens for study analyses, a 4 mL specimen for total bilirubin assessment will be drawn and the subject will provide a urine sample. The study coordinator will explain the Diet History questionnaires (DHQ) and administer the risk factor and adverse event (AE) questionnaires in order to obtain data on potential confounding dietary variables and gain subjects' baseline symptoms.

The study coordinator will provide the subject with a month' supply of either an SFN-rich broccoli sprout extract (BSE) capsule which consist of 200µmol of sulforaphane (SFN) or matching placebo, as dispensed by the Research Pharmacy. The matching placebo for the BSE consists of a gelatin capsule containing microcrystalline cellulose.

Around every 2 weeks, study coordinator will call to complete AE reporting and any changes in medications or supplements and complete brief cruciferous vegetable intake checklist. Subjects will return any unused study "drug" to the study coordinator at the time of biopsy (or at the 4 week visit if subject's prostate biopsy is delayed).

Eligibility criteria: Inclusion Criteria:

* Men scheduled for a prostate biopsy
* Age 21 years or older
* Signed informed subject consent

Exclusion Criteria:

* Definitive diagnosis with prostate cancer
* Significant active medical illness which in the opinion of the investigator or clinician would preclude protocol treatment
* Diagnosis of liver disease as noted on the patient problem list or baseline total bilirubin greater than institutional upper limit of normal
* Subject reported allergy or sensitivity to cruciferous vegetables
* Use of oral antibiotics, with the exception of doxycycline, within three months prior to randomization
* Use of warfarin or need for therapeutic anticoagulation at time of biopsy or at anytime during the course of the trial.
* Current oral steroid therapy
* Current therapy with valproate or other pharmacological drugs associated with HDAC inhibition
* Diagnosed dementia as noted on the patient problem list or other significant mental illness that may impact the subjects' ability to follow instructions or comply with the study protocol
* Patient may not be a part of another flagged study
* Patients already taking SFN dietary supplements

Primary outcomes:
measure: Change of Total Urine SFN (Sulforaphane) Metabolites
timeFrame: Baseline and 4-8 weeks following intervention
measure: Change of Total Plasma SFN (Sulforaphane) Metabolites Level
timeFrame: Baseline and 4-8 weeks following intervention
measure: Percentage of Ki67 Positive Cells up to 8 Weeks Post-randomization
timeFrame: Baseline and 4-8 weeks following intervention; prostate biopsy were collected post-intervention when clinically-indicated
measure: Expression of Histone Deacetylase 6 (HDAC6)
timeFrame: Baseline and 4-8 weeks following intervention; prostate biopsy were collected post-intervention when clinically-indicated
Secondary outcomes:
Other outcomes:
Locations:
city: Portland
country: United States
facility: OHSU Knight Cancer Institute
state: Oregon
city: Portland
country: United States
facility: Portland VA Medical Center
state: Oregon
Study officials:
affiliation: Portland VA Medical Center
name: Jackilen Shannon, PhD
role: PRINCIPAL_INVESTIGATOR
References:
citation: Zhang Z, Garzotto M, Davis EW 2nd, Mori M, Stoller WA, Farris PE, Wong CP, Beaver LM, Thomas GV, Williams DE, Dashwood RH, Hendrix DA, Ho E, Shannon J. Sulforaphane Bioavailability and Chemopreventive Activity in Men Presenting for Biopsy of the Prostate Gland: A Randomized Controlled Trial. Nutr Cancer. 2020;72(1):74-87. doi: 10.1080/01635581.2019.1619783. Epub 2019 Jun 1.