Clinical trial NCT03313869: A Nutrient Cocktail to Protect Against Physical Inactivity
Official title: Effects of a Cocktail XXS-2A/Omega-3 on Insulin Sensitivity and Oxidative Stress During a 20-day Period of Physical Inactivity: a Controlled, Randomized Pilot Study on 20 Healthy Men
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Metabolic Disturbance
Glucose Intolerance
Physical Inactivity
Glucose Metabolism Disorders
Lipid Metabolism Disorders
Oxidative Stress
Keywords: Metabolism
Lipid oxidation
micronutrient cocktail supplementation
physical inactivity
glucose intolerance
oxidative stress
Lead sponsor: Centre National d'Etudes Spatiales
Sponsor class: OTHER_GOV
First posted: 2017-10-18
Enrollment: 20
Sex: MALE
Minimum age (years): 20
Maximum age (years): 45
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: PARALLEL
Primary purpose: BASIC_SCIENCE

Brief summary: This experiment consists on a 20-day reduction in daily step in free-living active individuals to induce physical inactivity. This will be used to test the efficacy of the anti-oxidant cocktail we aim to test as a new countermeasure in 2016 during the 60-d bed rest planed by ESA/CNES.

The objective of this study is to investigate whether the cocktail of natural antioxidants XXS-2A comprising vitamin E and coupled with omega-3 helps to prevent and / or reduce the glucose intolerance and improve oxidative defenses induced by 20 days of physical inactivity through daily step reduction

Although physical inactivity is reported to affect glucose tolerance within days of inactivity, we selected a period of 20 days for the effect of the cocktail to take place and assess secondary molecular mechanisms. The effect of this short period of inactivity on metabolism will moreover be boosted during the last 10 days by taking fructose, a sugar found in abundance in fruits, honey and juices, which is known to quickly trigger metabolic deregulation.


Eligibility criteria: Inclusion Criteria:

* Healthy male subjects,
* Aged 20-45 years,
* No obesity or excessive thinness with BMI (weight Kg / height m2) between 22 and 27,
* Height (cm) between 158 and 190 cm,
* Certified as healthy by a comprehensive clinical assessment (detailed medical history and complete physical examination): in particular, free from any chronic disease or any acute infectious disease or ENT, neurological, orthopaedic, musculoskeletal and cardiovascular disorders,
* In the active range of population (10000 to 15000 steps/day)
* No contraindication to lidocaïne,
* No allergy to peanuts or soya,
* Non smokers or smoking less than 5 cigarettes/day,
* No alcohol, no drug dependence and no medical treatment,

Regulations

* Having given written informed consent prior to any procedure related to the study,
* Covered by Health Insurance System,
* Not under any administrative or legal supervision,
* Not under guardianship or trusteeship.

Exclusion Criteria:

* Any history or presence of clinically relevant cardiovascular, neurological or ENT, any chronic disease; any acute infectious disease,
* Obesity or excessive thinness,
* Not in the active range of population (\<10000 steps/day),
* Ongoing medical treatment,
* Poor tolerance to blood sampling,
* Having given blood (more than 8ml/kg) in a period of 8 weeks or less before the start of the experiment,
* Subject with contraindication to lidocaïne,
* Special food diet, vegetarian or vegan or food supplementation,
* History of food allergy, especially allergy to peanuts or soya,
* A significant history of allergy,
* Positive reaction to any of the following tests: HVA IgM (hepatitis A), HBs antigen (hepatitis B), anti-HVC antibodies (hepatitis C), anti-HIV1+2 antibodies,
* History or presence of drug or alcohol abuse (alcohol consumption \> 40 grams/day).

General conditions

* Subject who, in the judgment of the Investigator, is likely to be non-compliant during the study, or unable to cooperate because of a language problem or poor mental development,
* Subject in the exclusion period of a previous study according to applicable regulations,
* Subject who has received more than 4500 Euros within 12 months for being a research subject,
* Subject who cannot be contacted in case of emergency,
* Incarcerated persons,
* Subject under guardianship or trusteeship.

Primary outcomes:
measure: Change in Lipid oxidation
timeFrame: 20 days
Secondary outcomes:
measure: Change in glucose concentration
timeFrame: 20 days
measure: Change in insulin concentration
timeFrame: 20 days
measure: Change in NEFA
timeFrame: 20 days
measure: Change in triglycerides
timeFrame: 20 days
measure: de novo lipogenesis
timeFrame: 20 days
measure: Change in fructose oxidation
timeFrame: 20 days
measure: Change in oxidative stress
timeFrame: 20 days
measure: Change in fat-free mass
timeFrame: 20 days
measure: muscle pathways involved in intertwined protein synthesis / insulin sensitivity
timeFrame: 4h for each day of test. 12h for all the protocol
Other outcomes:
Locations:
city: Toulouse
country: France
facility: Medes-Imps
Study officials:
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