Clinical trial NCT05456022: Therapeutic Efficacy of Quercetin Versus Its Encapsulated Nanoparticle on Tongue Squamous Cell Carcinoma Cell Line
Official title: Therapeutic Efficacy of Quercetin Versus Its Encapsulated Nanoparticle on Tongue Squamous Cell Carcinoma Cell Line
Study type: INTERVENTIONAL
Overall status: UNKNOWN
Phase: PHASE2
Conditions: Oral Cancer
Keywords: Tongue
SCC
chemotherapy
Quercetin
PLGA-PEG nanoparticle
Lead sponsor: Cairo University
Sponsor class: OTHER
First posted: 2022-07-13
Enrollment: 1000000
Sex: ALL
Standard ages: CHILD
ADULT
OLDER_ADULT
Accepts healthy volunteers: no
Allocation: NON_RANDOMIZED
Intervention model: FACTORIAL
Primary purpose: TREATMENT

Brief summary: Squamous cell carcinoma (SCC) is the most common oral cavity carcinoma. Conventional therapeutic modalities for oral malignancy include surgery, radiotherapy and chemotherapy alone or in combinations.The major obstacle of using current anticancer drugs is; first the non-specific tissue distribution, as these drugs are unable to distinguish between normal and cancer cells.Quercetin is a bioactive flavonoid having strong antioxidant properties. .Among all the nanomaterials, polymeric nanoparticles are of significant interest for drug delivery applications due to many unique features of nanoparticle polymers.This is the first study to investigate the anticancer effects of (Quercetin) either free or encapsulated by PLGA-PEG NPs in tongue squamous cell carcinoma (TSCC) cell line.

Detailed description: Squamous cell carcinoma (SCC) is the most common oral cavity carcinoma. Conventional therapeutic modalities for oral malignancy include surgery, radiotherapy and chemotherapy alone or in combinations. The major obstacle of using current anticancer drugs is; first the non-specific tissue distribution, as these drugs are unable to distinguish between normal and cancer cells. Quercetin is a bioactive flavonoid having strong antioxidant properties. It is naturally present in a wide variety of fruits and vegetables. Among all the nanomaterials, polymeric nanoparticles are of significant interest for drug delivery applications due to many unique features of nanoparticle polymers. Polymeric nanocarriers have been fabricated from natural and synthetic polymers. Poly ethylene glycol-poly lactide-co-glycolic acid (PEG-PLGA) amphiphilic copolymer is an emergent system because it can be easily synthesized and possesses a lot of good qualities. Several previous in vitro and in vivo studies have evaluated the cytotoxic effects of quercetin and have revealed that it decreases cell viability and increases cell apoptotic rate in OSCC. However, the anti-cancer property of quercetin in tongue squamous cell carcinoma (TSCC) has not been studied yet. This is the first study to investigate the anticancer effects of (Quercetin) either free or encapsulated by PLGA-PEG NPs in tongue squamous cell carcinoma (TSCC) cell line.

Eligibility criteria: Inclusion Criteria:

* Tongue Squamous cell carcinoma cell lines
* Quercetin drug.
* Quercetin-encapsulated PLGA-PEG nanoparticles (Nano-QUT)
* Application of a Quercetin drug as chemotherapeutic drug.
* Detection of Quercetin activity in apoptosis or cytotoxicity/cell viability.

Exclusion Criteria:

* Any cancer cell line other than tongue Squamous cell carcinoma cell lines
* Any use of Quercetin other than chemotherapy.
* Detection of Quercetin activities other than apoptosis or cytotoxicity/cell viability

Primary outcomes:
measure: Cytotoxicity/Cell viability.
timeFrame: within 1 week after cell line propagation
measure: Apoptosis.
timeFrame: within 1 week after cell line propagation
Secondary outcomes:
measure: Gene expression of (BCL-2) .
timeFrame: within 1 week after cell line propagation
measure: Gene expression of ( Bax gene)
timeFrame: within 1 week after cell line propagation
measure: survival pathway (Expression of pi3k gene)
timeFrame: within 1 week after cell line propagation
Other outcomes:
Locations:
city: Cairo
country: Egypt
facility: 11 Saraya El Manial
Study officials:
References: