Clinical trial NCT01770938: Effects of Phototherapy by Low-level Light-emitting Diode Therapy (LEDT) on Clinical, Biochemical, Biomechanical, Histologic and Genetic Responses of Muscle Performance of Young Males Submitted to Physical Strength Training
Official title: Effects of Phototherapy by Low-level Light-emitting Diode Therapy (LEDT) on Clinical, Biochemical, Biomechanical, Histologic and Genetic Responses of Muscle Performance of Young Males Submitted to Physical Strength Training
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Healthy
Keywords: Light-emitting diode therapy
Muscle performance
Gene expression
Functional performance
Lead sponsor: Universidade Federal de Sao Carlos
Sponsor class: OTHER
Collaborators: name: Fundação de Amparo à Pesquisa do Estado de São Paulo
name: Centro de Pequisas de Ótica e Fotônica
First posted: 2013-01-18
Enrollment: 32
Sex: MALE
Minimum age (years): 18
Maximum age (years): 28
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: CROSSOVER
Primary purpose: TREATMENT

Brief summary: Muscle performance is largely influenced by modulations in gene expressions of muscle tissue. In this context, light-emitting diode therapy (LEDT) has been used to improve muscle performance in experimental models and human researches. Thereby, the investigators examined modulations in gene expression of muscle tissue as well as biochemical, biomechanic and histologic adaptations influenced by exercise associated to LEDT.

Detailed description: This study assessed muscle performance in isokinetic dynamometry and led press device, gene expression by RT-PCR, lactate, creatine kinase and morphology of muscle tissue by immunohistochemical analysis

Eligibility criteria: Inclusion Criteria:

* healthy males aged between 18 and 28 years with a body mass index (BMI) equal to or less than 26 kg/m2,
* moderated pattern of physical activity

Exclusion Criteria:

* previous injury to the femoral quadriceps or hamstring muscles (within 6 months prior to study),
* osseous or articular disorder in the lower limbs,
* cardiovascular system disorders,
* systemic disease, and
* taking prescription medicines or using dietary supplements such as muscle mass builders.

Primary outcomes:
measure: Modulations on muscle gene expression by RT-PCR
timeFrame: 12 weeks
Secondary outcomes:
measure: Muscle performance in leg press device
timeFrame: 12 weeks
measure: Muscle performance in leg extension device
timeFrame: 12 weeks
measure: Muscle performance in isokinetic dynamometer
timeFrame: 12 weeks
measure: Gene expression
timeFrame: 12 weeks
Other outcomes:
measure: Immunohistochemical analysis
timeFrame: 12 weeks
Locations:
city: São Carlos
country: Brazil
facility: Federal University of São Carlos
state: São Paulo
Study officials:
affiliation: Universidade Federal de Sao Carlos
name: Cleber Ferraresi, MSc
role: PRINCIPAL_INVESTIGATOR
affiliation: Universidade Federal de Sao Carlos
name: Nivaldo A Parizotto, PhD
role: STUDY_DIRECTOR
affiliation: University of Sao Paulo
name: Vanderlei S Bagnato, PhD
role: STUDY_DIRECTOR
References:
citation: Vieira WH, Ferraresi C, Perez SE, Baldissera V, Parizotto NA. Effects of low-level laser therapy (808 nm) on isokinetic muscle performance of young women submitted to endurance training: a randomized controlled clinical trial. Lasers Med Sci. 2012 Mar;27(2):497-504. doi: 10.1007/s10103-011-0984-0. Epub 2011 Aug 26.
citation: Ferraresi C, de Brito Oliveira T, de Oliveira Zafalon L, de Menezes Reiff RB, Baldissera V, de Andrade Perez SE, Matheucci Junior E, Parizotto NA. Effects of low level laser therapy (808 nm) on physical strength training in humans. Lasers Med Sci. 2011 May;26(3):349-58. doi: 10.1007/s10103-010-0855-0. Epub 2010 Nov 18.