Clinical trial NCT02436629: Nitrate for High Intensity Intermittent Exercise
Official title: The Effect of Nitrate Supplementation on Sport Performance: High Intensity Intermittent Performance in Recreational Athletes
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: PHASE4
Conditions: High Intensity Intermittent Sports Performance
Keywords: Red beetroot juice
High intensity intermittent exercise
Nitrite
Soccer
Lead sponsor: Maastricht University Medical Center
Sponsor class: OTHER
First posted: 2015-05-07
Enrollment: 40
Sex: MALE
Minimum age (years): 18
Maximum age (years): 40
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: CROSSOVER
Primary purpose: BASIC_SCIENCE

Brief summary: The objective of the current study is to assess the effect of 6-day dietary nitrate ingestion on high intensity intermittent sports performance in recreational soccer players.

Detailed description: Oral ingestion of nitrate (NO3-) in the form of both nitrate salts and concentrated red beetroot juice has been shown to significantly lower blood pressure at rest and to improve exercise performance during cycle time trials. Furthermore, a positive exercise performance effect of nitrate ingestion has been observed in recreational athletes during intermittent exercise. However, this was achieved after acute supplementation with a substantial dose of dietary nitrate. Whether similar ergogenic effects of nitrate supplementation can be achieved with a more conventional 6-day nitrate supplementation protocol with a lower daily dose remains to be established. Based on the gaps in current literature, our main goal will be to gain further insight into the effects of a 6-day nitrate supplementation protocol on high intensity intermittent exercise performance in recreational athletes.

Eligibility criteria: Inclusion Criteria:

* Healthy P
* Players of the first two senior selection teams of a regional soccer club competing at the 3rd KNVB level or higher
* Training ≥2 days a week
* 18-40 years
* Male

Exclusion Criteria:

* Use of medication
* Injury prohibiting them from performing the exercise protocol effectively
* Smoking
* Currently supplementing diet with nitrate
* Lactose intolerance

Primary outcomes:
measure: Total distance covered during the Yo-Yo Intermittent Recovery 1 test (Yo-Yo IR1 test)
timeFrame: Performance 3 hours post red beetroot juice ingestion
Secondary outcomes:
measure: Plasma concentration of nitrite and nitrate
timeFrame: Concetrations 3 hours post red beetroot juice ingestion
measure: Salivary concentration of nitrite and nitrate
timeFrame: Concetrations 3 hours post red beetroot juice ingestion
Other outcomes:
measure: Baseline characteristics
timeFrame: 30 min during visit 1 (Screening)
Locations:
city: Maastricht
country: Netherlands
facility: Maastricht University Medical Centre+
state: Limburg
Study officials:
affiliation: Maastricht University
name: Lex B Verdijk, PhD
role: PRINCIPAL_INVESTIGATOR
References:
citation: Wylie LJ, Mohr M, Krustrup P, Jackman SR, Ermiotadis G, Kelly J, Black MI, Bailey SJ, Vanhatalo A, Jones AM. Dietary nitrate supplementation improves team sport-specific intense intermittent exercise performance. Eur J Appl Physiol. 2013 Jul;113(7):1673-84. doi: 10.1007/s00421-013-2589-8. Epub 2013 Feb 1.
citation: Bond H, Morton L, Braakhuis AJ. Dietary nitrate supplementation improves rowing performance in well-trained rowers. Int J Sport Nutr Exerc Metab. 2012 Aug;22(4):251-6. doi: 10.1123/ijsnem.22.4.251. Epub 2012 Jun 15.
citation: Cermak NM, Gibala MJ, van Loon LJ. Nitrate supplementation's improvement of 10-km time-trial performance in trained cyclists. Int J Sport Nutr Exerc Metab. 2012 Feb;22(1):64-71. doi: 10.1123/ijsnem.22.1.64.
citation: Bangsbo J, Iaia FM, Krustrup P. The Yo-Yo intermittent recovery test : a useful tool for evaluation of physical performance in intermittent sports. Sports Med. 2008;38(1):37-51. doi: 10.2165/00007256-200838010-00004.