Clinical trial NCT03229044: Tetranectin as a Novel Obesity-associated Adipokine for Regulating Lipid Accumulation
Official title: Tetranectin as a Novel Obesity-associated Adipokine for Regulating Lipid Accumulation
Study type: OBSERVATIONAL
Overall status: COMPLETED
Conditions: Obesity
Metabolic Syndrome
Keywords: adipokine, tetranectin, lipid metabolism, body mass index
Lead sponsor: National Taiwan University Hospital
Sponsor class: OTHER
First posted: 2017-07-25
Enrollment: 289
Sex: ALL
Minimum age (years): 20
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: yes
Observational model: CASE_ONLY
Time perspective: RETROSPECTIVE
Sampling method: PROBABILITY_SAMPLE
Study population: Adipose tissues were obtained from the breast of breast cancer patients with 0 to 1 progression stage.

Brief summary: Adipose tissue is strongly correlated to obesity and diabetes which are two major problems in the worldwide. Adipose tissue is involved in modulating glucose and energy metabolism locally and systemically by secreting factors, called adipocytokines. Tetranectin (TN), a novel adipocytokine, is secreted by adipose tissues. TN may regulate the glucose/energy homeostasis to affect lipid accumulation.The role of TN in adipocytes with or without TN on regulation of adipose tissue growth and lipid metabolism is valuable to be studied.

Materials and methods: Secreted proteins from human adipocyte culture medium were deciphered using 2-D polyacrylamide gel electrophoresis and determined by LC/MS with MALDI-TOF analysis of proteins. Adipose tissues were obtained from the breast of 15 women undergoing mastectomy. Serum samples from 200 normal healthy people and 54 breast cancer patients were assigned to either a lean or obese group according to the body mass index. The TN protein concentration was measured in the culture medium of stromal-vascular fraction (SVF)-derived adipocytes from healthy humans, breast cancer patients, pigs and also from 3T3-L1 cells. Associations between plasma TN and other biomarkers were assessed with Pearson's correlations and multivariable linear regression.

Detailed description: Adipose tissues were obtained from the breast of breast cancer patients with 0 to 1 progression stage. All participants gave permission and the study was approved by the Ethics Committee of National Taiwan University Hospital as no. 9461701203.

From 2012 to 2013 in National Taiwan University Hospital, a cross-sectional sampling of 21 to 78 -year old people included women and men who were living in Taiwan. Blood samples were collected after an overnight fasting. Serum was separated by centrifugation and stored at -80 ℃ until analysis. Plasma triglyceride (TG), high density lipoprotein (HDL), low density lipoprotein (LDL), cholesterol, glucose and insulin were measured. Fasting serum samples were obtained from 193 randomly selected persons having health check-ups. To investigate the relationship among TN and breast cancer, fasting serum samples also obtained from 54 randomly selected breast cancer patients who were 25 to 59 years old with elected surgery for breast cancer stage 0 and 1.

Eligibility criteria: Inclusion Criteria:

1. Must be above age 20 years old.
2. Subjects must sign the written informed consent form.
3. Must have residual fat after surgery.
4. Clinical diagnosis of liposuction and tumor resection.

Exclusion Criteria:

1. Terminal cancer.
2. Subjects have no fat tissue after surgery.
3. Pregnant subjects.

Primary outcomes:
measure: Tetranectin expression measure
timeFrame: through study completion, an average of 2 years
Secondary outcomes:
Other outcomes:
Locations:
Study officials:
affiliation: National Taiwan University
name: Shih-Torng Ding, Ph D
role: PRINCIPAL_INVESTIGATOR
References:
citation: Jaquinod M, Holtet TL, Etzerodt M, Clemmensen I, Thogersen HC, Roepstorff P. Mass spectrometric characterisation of post-translational modification and genetic variation in human tetranectin. Biol Chem. 1999 Nov;380(11):1307-14. doi: 10.1515/BC.1999.166.
citation: Iba K, Durkin ME, Johnsen L, Hunziker E, Damgaard-Pedersen K, Zhang H, Engvall E, Albrechtsen R, Wewer UM. Mice with a targeted deletion of the tetranectin gene exhibit a spinal deformity. Mol Cell Biol. 2001 Nov;21(22):7817-25. doi: 10.1128/MCB.21.22.7817-7825.2001.