Clinical trial NCT04213586: Effects of Whey Protein and Collagen Supplementation
Official title: Effects of Whey Protein and Collagen Supplementation on Muscle Mass and Function During Training in Young Adults
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Exercise
Nutrition Therapy
Resistance Training
Muscle Strength
Hypertrophy
Keywords: Protein supplementation
Amino acids
Resistance exercise
Muscle strength
Muscle hypertrophy
Lead sponsor: Universidade Norte do Paraná
Sponsor class: OTHER
Collaborators: name: Universidade Estadual de Londrina
First posted: 2019-12-30
Enrollment: 22
Sex: ALL
Minimum age (years): 18
Maximum age (years): 35
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: PARALLEL
Primary purpose: BASIC_SCIENCE

Brief summary: The present project aims to investigate the effects of high-quality protein (whey protein) vs. low-quality protein (collagen) on muscle mass and function (e.g., strength and power) during a 10-wk resistance training program in young adults. It will be tested the hypothesis that leucine-matched collagen should promote similar gains on muscle function and mass compared to whey protein supplementation.

Detailed description: The purpose of this study will be to investigate the effects of whey protein vs. leucine-matched collagen supplementation on muscle mass and function after a 10-wk resistance training program. Initially, all participants will be submitted for a 2-wk familiarization period, and then randomized into two groups (N = 11 / group): whey protein group and leucine-matched collagen group. The following variables will be assessed from pre-to post-training: anthropometric, muscle thickness from vastus lateralis and biceps brachii muscles using ultrasound, training load, mean power and peak torque for unilateral arm flexion exercise using a isokinetic dynamometer, countermovement vertical jump, nutritional intake, and testosterone-to-cortisol ratio . Shapiro-Wilk and Levine tests will be used to test the normality and homogeneity of the measurements, respectively. Two-way ANOVA (group x time) tests for repeated measures will be performed to assess changes over time and between groups for all dependent variables. Violation of sphericity will adjust using Greenhouse- Geyser correction. The Bonferroni post-hoc test will detect specific differences between groups. When appropriate, the percentage of change (∆%) will be reported according to the following equation: ∆% = \[(pre-average post average) / pre-average mean\] × 100. Values will be express as mean ± standard deviation. O significance level α will be 0.05.

Eligibility criteria: Inclusion Criteria:

* Men and women aged 18-35 years
* To be eutrophic (not overweight or obese)

Exclusion Criteria:

* To present a history of musculoskeletal disorders
* Be attending a systematized weight training program for at least six months
* To make use of medicines that could affect muscle function.
* To have used ergogenic supplements and anabolic steroids for at least six months before study
* To have restrictions for exercises practice considering Physical Activity Readiness Questionnaire (PAR-Q) responses.

Primary outcomes:
measure: Change in biceps brachii thickness
timeFrame: Baseline and after 10 weeks
measure: Change in muscle strength by training load
timeFrame: Baseline, 5 and 10 weeks
measure: Change in vertical jump
timeFrame: Baseline and after 10 weeks
measure: Change in mean power
timeFrame: Baseline and after 10 weeks
measure: Change in peak torque
timeFrame: Baseline and after 10 weeks
measure: Change in vastus lateralis thickness
timeFrame: Baseline and after 10 weeks
Secondary outcomes:
measure: Change in testosterone-to-cortisol ratio
timeFrame: Baseline and after 10 weeks
measure: Dietary intake
timeFrame: Baseline, 2, 5, 10 weeks
Other outcomes:
Locations:
city: Londrina
country: Brazil
facility: Universidade Norte do Paraná
state: Paraná
Study officials:
affiliation: Universidade Norte do Paraná
name: Andreo Aguiar
role: PRINCIPAL_INVESTIGATOR
References: