Clinical trial NCT01366924: Essential Amino Acids Supplementation and Muscle Protein Synthesis
Official title: The Effect of Amino Acid Supplementation on Skeletal Muscle Protein Turnover Following Endurance Exercise
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: PHASE1
PHASE2
Conditions: Muscle Loss
Muscle Anabolism
Keywords: protein turnover
whole body protein turnover
essential amino acids
leucine
Lead sponsor: United States Army Research Institute of Environmental Medicine
Sponsor class: FED
Collaborators: name: Tufts University
First posted: 2011-06-06
Enrollment: 23
Sex: ALL
Minimum age (years): 18
Maximum age (years): 35
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: PARALLEL
Primary purpose: BASIC_SCIENCE

Brief summary: The modern warfighter faces numerous physiological challenges including sleep deprivation, sustained intense physical activity, and caloric restriction, the combined effects of which may result in the loss of lean body mass and decreased physical performance. Dietary interventions may help preserve lean body mass and facilitate recovery from periods of intense physical demand. For example, dietary strategies that increase amino acid availability have been shown to stimulate protein synthesis in skeletal muscle following resistance exercise. Because military tasks also incorporate endurance exercise components, studies regarding the effects of increasing dietary amino acids following endurance exercise are warranted. The objectives of this study are to characterize the effect of endurance exercise on protein synthesis and breakdown as well as the ability of an essential amino acid supplement to influence skeletal muscle protein metabolism and its cellular and molecular regulation following endurance exercise.


Eligibility criteria: Inclusion Criteria:

* Men and women aged 18 - 35 years
* Weight stable
* Recreationally active with VO2max of 40 - 50 ml/kg/min
* Refrain from taking any NSAIDS or any aspirin-containing medications
* Supervisor approval if a federal employee

Exclusion Criteria:

* Disease or medication that affects macronutrient metabolism and/or the ability to participate in strenuous exercise
* Allergies to foods or medications (including, but not limited to, lidocaine or phenylalanine) to be utilized in the study
* Abnormal PT/PTT test or problems with blood clotting
* Present condition of alcoholism or other substance abuse that compromises exercise capacity
* Musculoskeletal injuries that compromise the ability to exercise
* Pregnancy

Primary outcomes:
measure: Change in muscle protein synthesis after exercise or exercise with amino acid supplementation.
timeFrame: An average change over a 210 minute recovery period from a 60 minute endurance exercise session
Secondary outcomes:
measure: Change in intracellular signaling after exercise or exercise with amino acid supplementation.
timeFrame: At 30 minutes and 210 minutes after a 60 minute endurance exercise session
Other outcomes:
Locations:
city: Boston
country: United States
facility: Tufts University
state: Massachusetts
city: Natick
country: United States
facility: US Army Research Institute of Environmental Medicine
state: Massachusetts
Study officials:
affiliation: US Army Research Institute of Environmental Medicine
name: Stefan M Pasiakos, Ph.D.
role: PRINCIPAL_INVESTIGATOR
References:
citation: Pasiakos SM, McClung HL, McClung JP, Margolis LM, Andersen NE, Cloutier GJ, Pikosky MA, Rood JC, Fielding RA, Young AJ. Leucine-enriched essential amino acid supplementation during moderate steady state exercise enhances postexercise muscle protein synthesis. Am J Clin Nutr. 2011 Sep;94(3):809-18. doi: 10.3945/ajcn.111.017061. Epub 2011 Jul 20.
citation: Pasiakos SM, McClung HL, McClung JP, Urso ML, Pikosky MA, Cloutier GJ, Fielding RA, Young AJ. Molecular responses to moderate endurance exercise in skeletal muscle. Int J Sport Nutr Exerc Metab. 2010 Aug;20(4):282-90. doi: 10.1123/ijsnem.20.4.282.
citation: Scofield DE, McClung HL, McClung JP, Kraemer WJ, Rarick KR, Pierce JR, Cloutier GJ, Fielding RA, Matheny RW Jr, Young AJ, Nindl BC. A novel, noninvasive transdermal fluid sampling methodology: IGF-I measurement following exercise. Am J Physiol Regul Integr Comp Physiol. 2011 Jun;300(6):R1326-32. doi: 10.1152/ajpregu.00313.2010. Epub 2011 Mar 9.