Clinical trial NCT02352740: Characterization of the Metabolic Fate of an Oral Arginine Form
Official title: Characterization of the Metabolic Fate of an Oral L-arginine Form in Healthy Subjects Featuring Risk Factors Related to the Metabolic Syndrome.
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Overweight
Hypertriglyceridemic Waist
Keywords: arginine
supplementation
metabolic syndrome
nitric oxide
Lead sponsor: Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
Sponsor class: OTHER
Collaborators: name: Institut de Recherche Pierre Fabre
name: Hospital Avicenne
name: Adeprina
First posted: 2015-02-02
Enrollment: 32
Sex: ALL
Minimum age (years): 18
Maximum age (years): 60
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: CROSSOVER

Brief summary: The purpose of this study is to compare the metabolic fate of two oral forms of L-Arginine in healthy subjects featuring metabolic syndrome related risk factors

Detailed description: The study is a randomized crossover study including 16 healthy subjects with risk factors for metabolic syndrome and 16 healthy control subjects. According a double crossover design, each subject received two oral forms of L-arginine (A and B) in random order, and participated in a exploration day on the first day of arginine administration and after one week of supplementation with this arginine form. The two weeks of arginine supplementation were separated by a washout period of 2 weeks at least.

Each exploration extended over 24 hours after administration of the first arginine dose. Blood tests were performed at 0, 0.5, 1, 2, 4, 6, 8, 12, 16, 24 h after administration of the first dose. During explorations after the supplementation period, we also collected urine (0, 2, 4, 8, 12, 24 h after the first dose).

Eligibility criteria: Healthy subjects with 'Hypertriglyceridemic waist' :

Inclusion Criteria:

* Age between 18 to 60 years old
* Overweight (BMI between 25 and 30 kg/m²)
* 'Hypertriglyceridemic waist' (waist circumference \> 94cm for men or \> 88cm for women and fasting triglyceride levels \> 150 mg/dL)

Exclusion Criteria:

* Obesity (BMI\> 30 kg / m²)
* Cardiac or vascular diseases
* Diabetes
* Thyroid disease
* Systolic blood pressure \> 150 mmHg or diastolic blood pressure \> 90 mmHg
* Tobacco consumption \> 6 cigarettes per week
* Alcohol consumption\> 3 drinks per day
* Any medication (except contraceptive treatment) or dietary supplement intake that could not be arrested more than a week before the first visit for the duration of the study.
* Persons under guardianship
* Pregnancy (positive beta-hCG blood test)
* Positive serology HBsAg AcHbc, HCV and HIV
* Hemoglobin \< 14 g/dl (for men) or \<12 g / dl (for women)
* Participation in a clinical trial within 6 months preceding the study

Healthy control subjects :

Inclusion Criteria:

* Age between 18 to 60 years old
* Normal weight (BMI between 18.5 and 25 kg/m²)
* Waist circumference \< 94cm for men or \< 88cm for women and fasting triglyceride levels \< 150 mg/dL

Exclusion Criteria :

* Cardiac or vascular diseases
* Diabetes
* Thyroid disease
* Systolic blood pressure \> 150 mmHg or diastolic blood pressure \> 90 mmHg
* Tobacco consumption \> 6 cigarettes per week
* Alcohol consumption\> 3 drinks per day
* Any medication (except contraceptive treatment) or dietary supplement intake that could not be arrested more than a week before the first visit for the duration of the study.
* Persons under guardianship
* Pregnancy (positive beta-hCG blood test)
* Positive serology HBsAg AcHbc, HCV and HIV
* Hemoglobin \< 14 g/dl (for men) or \<12 g / dl (for women)
* Participation in a clinical trial within 6 months preceding the study

Primary outcomes:
measure: Estimate of total conversion of a dose of oral arginine into NO
timeFrame: Repeated measurement for 24h before (day 0) and after supplementation (day 8) for each treatment
measure: Estimate of kinetic profiles of plasma arginine concentrations over 24 hours
timeFrame: Repeated measurement for 24h before (day 0) and after supplementation (day 8) for each treatment
Secondary outcomes:
measure: Quantitative analysis of plasma markers of endothelial function
timeFrame: Before supplementation (day 0) and after supplementation (day 8) for each treatment
measure: Estimate of kinetics use of arginine for NO and urea synthesis
timeFrame: Repeated measurement for 24h after supplementation (day 8) for each treament
measure: Other quantitative analysis
timeFrame: Before supplementation (day 0) and after supplementation (day 8) for each treatment
Other outcomes:
Locations:
city: Bobigny
country: France
facility: Centre de Recherche sur Volontaires (CRV), Hospital Avicenne
state: Ile-de-France
Study officials:
affiliation: Hospital Avicenne
name: Robert Benamouzig
role: PRINCIPAL_INVESTIGATOR
affiliation: AgroParisTech
name: François Mariotti, PhD
role: STUDY_DIRECTOR
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