Clinical trial NCT02057822: Cytokine Assay in Tears of Healthy Children and With Vernal Keratoconjunctivitis: Case Control Study and Monitoring of Cases at 6 Months
Official title: Cytokine Assay in Tears of Healthy Children and With Vernal Keratocunjonctivitis: Case Control Study and Monitoring of Cases at 6 Months
Study type: OBSERVATIONAL
Overall status: UNKNOWN
Conditions: Vernal Keratoconjunctivitis
Healthy Children
Keywords: Vernal keratoconjunctivitis
Cytokines
Mediators
Tears
Lead sponsor: University Hospital, Clermont-Ferrand
Sponsor class: OTHER
First posted: 2014-02-07
Enrollment: 60
Sex: ALL
Minimum age (years): 3
Maximum age (years): 18
Standard ages: CHILD
ADULT
Accepts healthy volunteers: yes
Time perspective: PROSPECTIVE
Sampling method: NON_PROBABILITY_SAMPLE
Study population: healthy children children with vernal keratoconjunctivitis

Brief summary: The ocular surface may be the seat of an inflammatory process in many pathology as vernal keratoconjunctivitis. It has been shown that inflammatory cytokines produced by the cells themselves and the constituent cells of the target organ, are the main factors that cause inflammation. The main objective of our study was to compare the concentration of 40 cytokines in vernal keratoconjunctivitis and in control subjects.

Detailed description: The ocular surface may be the seat of an inflammatory process in many pathology as vernal keratoconjunctivitis. It has been shown that inflammatory cytokines produced by the cells themselves and the constituent cells of the target organ, are the main factors that cause inflammation. The main objective of our study was to compare the concentration of 40 cytokines in vernal keratoconjunctivitis and in control subjects.

Eligibility criteria: Inclusion Criteria:

* Childrens between 3 and 18 years
* Affiliated at system of French social security
* Signed by the holders of parental authority of the sheet and written consent to participate in this study
* First consultation at Clermont-Ferrand Hospital for vernal keratocunjunctivitis

Exclusion Criteria:

* Subject suffering from acute or chronic eye disease in which the physician investigator resound with the results: uveitis, glaucoma, retinopathy
* Subject who had undergone eye surgery
* Subject having an infection the day of collection: ENT, digestive, urinary
* Subject with cancer, mental illness or any other condition in which the physician investigator sound informed consent and / or the results
* Pregnant subject
* Subject with contact lenses
* Subject who judged by the investigating physician not enough cooperating to allow a safe collection

Primary outcomes:
measure: Concentration of 40 cytokines in tears
timeFrame: at time 0
Secondary outcomes:
measure: Validation of normal range of cytokines in tears of healthy children
timeFrame: at 6 month
measure: Modification of the inflammatory profile according to the clinical severity and treatment
timeFrame: at 6 month
Other outcomes:
Locations:
city: Clermont-Ferrand
country: France
facility: CHU de Clermont-Ferrand
Study officials:
affiliation: University Hospital, Clermont-Ferrand
name: Frédéric CHIAMBARETTA
role: PRINCIPAL_INVESTIGATOR
References: