Clinical trial NCT02364869: Prebiotic in Chronic Kidney Disease Patients
Official title: Effect of Prebiotic (Fructooligosaccharide) on Uremic Toxins and Cardiovascular Markers of Chronic Kidney Disease Patients
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Chronic Kidney Disease
Keywords: P-cresyl sulfate
Indoxyl sulfate
Intestine
Endothelial function
Lead sponsor: Federal University of São Paulo
Sponsor class: OTHER
Collaborators: name: Universidade Federal do Paraná
name: Pontifícia Universidade Católica do Paraná
First posted: 2015-02-18
Enrollment: 50
Sex: ALL
Minimum age (years): 18
Maximum age (years): 80
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: no
Allocation: RANDOMIZED
Intervention model: PARALLEL
Primary purpose: TREATMENT

Brief summary: This 12-week double-blind randomized controlled clinical trial aims to investigate the effect of a prebiotic (fructooligosaccharide - FOS) on serum and urinary levels of uremic toxins (p-cresyl sulfate and indoxyl sulfate) of non-dialysis dependent CKD patients, and the impact of such intervention on cardiovascular markers, intestinal permeability, endotoxemia and inflammatory response.

Detailed description: Intestinal microbiome has been considered a new therapeutic target for chronic kidney disease (CKD) due to its potential role on the metabolic disturbances associated to the disease. The abnormalities in the microbiota, frequently found in patients with CKD, contribute to the accumulation of uremic toxins derived from the unbalanced fermentation of nitrogen compounds in relation to the non-digestible carbohydrates. Among them, p-cresyl sulfate and indoxyl sulfate have been associated with inflammation, kidney disease progression, endothelial dysfunction and increased risk of death in this population. Preliminary studies especially on hemodialysis have shown that the use of prebiotic, probiotic and symbiotic may represent a promising intervention due to their beneficial effect as modulators of the intestinal microbiota that might promote a reduction on serum concentration of p-cresyl sulfate and indoxyl sulfate. In comparison to probiotic, prebiotic have the advantage to stimulate the host's microbiota and to occur naturally in several foods. In the context of CKD, the use of prebiotics has been poorly investigated. Therefore, the primary aim of this study is to investigate the effect of a prebiotic (fructooligosaccharide - FOS) on serum and urinary levels of p-cresyl sulfate and indoxyl sulfate of non-dialysis dependent CKD patients. As a secondary aim we will investigate the impact of such intervention on cardiovascular markers, intestinal permeability, endotoxemia and inflammatory response. This is a 12-week double-blind randomized controlled clinical trial. Fifty non-diabetic patients with CKD stages 3a and 4 will be randomly assigned to a 12 g/day of FOS or maltodextrin (placebo). The serum and urinary concentrations of p-cresyl sulfate and indoxyl sulfate will be determined by high performance liquid chromatography (HPLC). The assessment of endothelial function includes ultrasonography of the brachial artery, measurement of plasma and urinary nitric oxide, monocyte chemoattractant protein 1 (MCP1), stromal cell-derived factor 1 alpha (SDF1α), oxide - trimethylamine N- (TMAO), ambulatory blood pressure monitoring (ABPM) and pulse wave velocity (PWV). The serum intestinal trophic markers (glucagon-like peptide 2 - GLP2 - and epidermal growth factor - EGF), intestinal permeability (Zonulin), endotoxemia and inflammation (IL-6 and CRP) will be determined by ELISA. Food intake will be assessed by 3-day food records. Protein intake will be estimated by calculating the protein equivalent of nitrogen appearance (PNA). The Bristol Scale, the Roma III Criteria and the Gastrointestinal Symptoms Rating Scale will be applied to evaluate gastrointestinal effects during the follow-up. The subjective global assessment questionnaire, the spectroscopic bioimpedance analysis and the handgrip strength will be applied to evaluate the nutritional status of the patients.

Eligibility criteria: Inclusion Criteria:

* 18 to 80 years
* Estimated glomerular filtration rate between 45 to 15 ml/min/1,73m².

Exclusion Criteria:

* diabetes mellitus, malignance, severe liver disease, autoimmune diseases, congestive heart failure class III / IV, HIV, history of gastrointestinal disease
* use of phosphate binders, laxatives or prebiotic, probiotic, symbiotic, antibiotics, immunosuppressants and / or anti-inflammatory drugs three months preceding the study.
* patients using laxatives who refuse to stop treatment during the follow-up

Primary outcomes:
measure: Uremic toxicity evaluated by serum and urinary levels of p-cresyl sulfate and indoxyl sulfate
timeFrame: 12 weeks
Secondary outcomes:
measure: Endotoxemia measured by serum levels of lipopolysaccharides
timeFrame: 12 weeks
measure: Intestinal permeability evaluated by serum levels of Zonulin
timeFrame: 12 weeks
measure: Intestinal epithelium evaluated by serum levels of glucagon-like peptide 2 (GLP2)
timeFrame: 12 weeks
measure: Intestinal epithelium evaluated by serum levels of epidermal growth factor (EGF).
timeFrame: 12 weeks
measure: Inflammation measured by serum levels of Interleukin-6 (IL-6)
timeFrame: 12 weeks
measure: Inflammation measured by serum levels of c-reactive protein (CRP)
timeFrame: 12 weeks
measure: Endothelial function evaluated by ultrasonography of the brachial artery
timeFrame: 12 weeks
measure: Endothelial function evaluated by blood pressure monitoring (ABPM)
timeFrame: 12 weeks
measure: Endothelial function evaluated by pulse wave velocity (PWV).
timeFrame: 12 weeks
measure: Endothelial function evaluated by plasma and urinary levels of nitric oxide
timeFrame: 12 weeks
measure: Endothelial function evaluated by plasma and urinary levels of monocyte chemoattractant protein 1 (MCP1)
timeFrame: 12 weeks
measure: Endothelial function evaluated by plasma and urinary levels of stromal cell-derived factor 1 alpha (SDF1α)
timeFrame: 12 weeks
measure: Endothelial function evaluated by plasma and urinary levels of oxide - trimethylamine N- (TMAO)
timeFrame: 12 weeks
Other outcomes:
Locations:
city: São Paulo
country: Brazil
facility: Federal University of São Paulo
Study officials:
affiliation: Affiliate professor of Federal University of São Paulo
name: Lilian Cupari, PhD
role: PRINCIPAL_INVESTIGATOR
References: