Clinical trial NCT05626192: Anatomical Evaluation of the APL Tendons in Thumb CMC Joint and Osteoarthritis
Official title: Anatomical Evaluation of the APL and FCR Tendons in Thumb CMC Joint in Relation to Osteophytes Formation of the Trapezium and Osteoarthritis.
Study type: OBSERVATIONAL
Overall status: RECRUITING
Conditions: Thumb Osteoarthritis
Keywords: Thumb Osteoarthritis
Enthesis organ
Sensory nerve endings
Lead sponsor: Fundació Institut de Recerca de l'Hospital de la Santa Creu i Sant Pau
Sponsor class: OTHER
First posted: 2022-11-23
Enrollment: 20
Sex: ALL
Minimum age (years): 50
Maximum age (years): 90
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: no
Observational model: COHORT
Time perspective: PROSPECTIVE
Sampling method: NON_PROBABILITY_SAMPLE
Study population: The present study will include 20 specimens. The first part of the study will be a dissection of the specimens. Description of the variability of tendons in the first extensor compartment. The investigators analyze the APL tendon insertion, osteophytes formation and osteoarthritis in thumb CMC joint . In the second part of the study, the researchers will take a small sample of the APL insertion with the osteophyte, capsule and ligaments in the thumb CMC joint. The investigators will fix the sample in a solution of 4% formaldehyde and they will stain the samples with hematoxylin eosin. Immunohistochemistry with PGP 9.5 and S-100.

Brief summary: Certain ligaments in the human body are designed to be a static stabilizer and others to have a sensory function thus remains an unresolved issue.

The presence of mechanoreceptors (sensory corpuscles and free nerve endins) in the human knee and ankle ligaments has implies a sensory role of ligaments in providing afferent information, which in turn regulates intrinsic stiffness in the muscles surrounding the joint, contribuiting to dynamic joint stability.

The aim of this study is to use immunohistochemical methods to analyze the general innervation and possible existence of sensory corpuscles in the thumb carpometacarpal (CMC) joint. This study can help to know the contribution of the tendon insertions and ligaments in the dynamic thumb CMC joint stability.

Detailed description: The accessory APL tendons insertions, number of accessory tendons and insertion in trapezium can be a mechanical factor that develop osteophytes and thumb CMC joint osteoarthritis.

Antibodies against the general nerve marker protein gene product 9.5 (PGP 9.5) and the glial marker S-100 will be use to depict innervation and corpuscular structures. Innervation pattern and sensory corpuscules were investigated by other authors in the wrist. S-100 immunoreactivity is in the Schwann cells in the central regions of the corpuscle, and PGP 9.5 immunoreactivity marked the axonal structures in the corpuscles. The presence of nerve fascicles and particularly sensory corpuscles in the structures, for exemple capsule and APL insertion near the thumb carpometacarpal joint suggest that these structures in the CMC joint have a propioceptive role in the stability of the joint.

Eligibility criteria: Inclusion Criteria:

* Specimens: Adults from 50 to 90 years.

Exclusion Criteria:

* Specimens: younger than 50 or older than 90 years.

Primary outcomes:
measure: Number of APL tendon insertions in trapezium in thumb CMC joint
timeFrame: 12 months
measure: Osteophytes formation and thumb CMC joint osteoarthritis (OA)
timeFrame: 12 months
Secondary outcomes:
measure: Sensory nerve endings and mechanoreceptors in thumb CMC joint
timeFrame: 12 months
Other outcomes:
Locations:
city: Barcelona
country: Spain
facility: Claudia Erika Delgado Espinoza
city: Barcelona
country: Spain
facility: Hospital de la Santa Creu i Sant Pau
Study officials:
affiliation: Fundació Institut de Recerca de l'Hospital de la Santa Creu i Sant Pau
name: Claudia Lamas, MD, Ph D
role: PRINCIPAL_INVESTIGATOR
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