Clinical trial NCT05975489: Genetics in the Effect of Caffeine on Fat Oxidation
Official title: Influence of the Genetic Polymorphisms in the Effect of Caffeine on Fat Oxidation During Exercise
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Genetic Predisposition
Caffeine
Fat Burn
Lead sponsor: Universidad Francisco de Vitoria
Sponsor class: OTHER
First posted: 2023-08-04
Enrollment: 32
Sex: ALL
Minimum age (years): 18
Maximum age (years): 40
Standard ages: ADULT
Accepts healthy volunteers: no
Allocation: RANDOMIZED
Intervention model: PARALLEL
Primary purpose: BASIC_SCIENCE

Brief summary: Genetic polymorphism on the effect of oral caffeine intake on fat oxidation during exercise has been studied in active and healthy population performing an incremental test on a cycle ergometer with 3-min stages at workloads from 30 to 70% of maximal oxygen uptake (VO2max). Participants performed this test after the ingestion of a) placebo; b) 3 mg/kg of caffeine; c) 6 mg/kg of caffeine. Fat oxidation rate during exercise was measured by indirect calorimetry. The influence of the CYP1A2 c.-163A\>C, GSTP c.313A\>G and PGC1a polymorphisms was evaluated to determine the effects on fat oxidation during exercise

Detailed description: Caffeine is a natural stimulant with well-recognized sports performance benefits. Aside its performance-enhancing effect, caffeine has the potential of increasing fat utilization during aerobic exercise at submaximal intensities, lowering-down the contribution of carbohydrate as a fuel. This property of caffeine may provoke a glycogen-sparing effect in the skeletal muscle and liver for exercise situations where carbohydrate availability may be a challenge. Additionally, the capacity of caffeine to enhance fat utilization during exercise could be of interest for improving health outcomes as it may increase the rate of change in body composition in exercise programs. Genetic factors like CYP1A2 c.-163A\>C, GSTP c.313A\>G and PGC1a c.1444G\>A and C\>T polymorphisms could be associated with the capacity for fat oxidation during exercise. To date, it is unknown if genetics increases fat oxidation and MFO in the same proportion during morning and evening exercise trials in women. For this reason, the aim of the present study was to evaluate the influence of the tCYP1A2, GSTP and PGC1a polymorphisms on the effect of caffeine on fat oxidation and MFO in active and healthy population. The authors hypothesised that genetics would increase fat oxidation and MFO during exercise and this effect would be of similar magnitude at several caffeine doses.

Eligibility criteria: Inclusion Criteria:

To be non-smokers. To have low caffeine intake (i.e., \< 50 mg of caffeine per day in the previous 2 months) To show no previous history of cardiopulmonary diseases or having suffered musculoskeletal injuries in the previous 6 months.

Exclusion Criteria:

To have VO2max values below 40 ml/kg/min To be sedentary

Primary outcomes:
measure: Genotype frequency of CYP1A2 polymorphism
timeFrame: Baseline
measure: Genotype frequency of GSTP polymorphism
timeFrame: Baseline
measure: Genotype frequency of PGC1a polymorphisms
timeFrame: Baseline
Secondary outcomes:
measure: FATmax
timeFrame: 2-months
measure: MFO
timeFrame: 2-months
measure: RPE
timeFrame: 2-months
measure: FAT and CHO oxidation
timeFrame: 2-months
Other outcomes:
Locations:
city: Pozuelo De Alarcón
country: Spain
facility: Universidad Francisco de Vitoria
state: Madrid
Study officials:
affiliation: Universidad Francisco de Vitoria, crta Pozuelo-Majadahonda km 1.800 PC 28223, Madrid, Spain
name: David Varillas Delgado
role: PRINCIPAL_INVESTIGATOR
References: