Clinical trial NCT02040363: Muscle Training Induced Angiogenesis in COPD
Official title: Impact of Exercise Training on Skeletal Muscle Capillary Maturation During Angiogenesis in COPD Patients
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Peripheral Muscle Dysfunction
COPD
Keywords: COPD
skeletal muscle
angiogenesis
exercise training
intensity
Lead sponsor: University Hospital, Montpellier
Sponsor class: OTHER
Collaborators: name: Institut National de la Santé Et de la Recherche Médicale, France
name: Université Montpellier
name: 5 Santé
First posted: 2014-01-20
Enrollment: 22
Sex: ALL
Minimum age (years): 40
Maximum age (years): 78
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: PARALLEL
Primary purpose: BASIC_SCIENCE

Brief summary: COPD patients experiment a peripheral muscle dysfunction which impact their exercise tolerance and health-related quality of life. The capillary to fiber interface is reduced and impact the exercise capacity of the patients. While the muscle capillary creation in response to exercise training is blunted in COPD patients, the maturation of the neo-capillaries could also be blunted and contribute to the impaired aniogionenesis in patients. Because the capillary maturation is a sensitive and dynamic process, only different modalities of exercise training and multiple time-points of measures would allow to capture this microvascular adaptation.

Aim of the study : Compare the muscle capillary maturation in response to training at 5 and 10 weeks, in sedentary healthy subject trained at the intensity of the ventilatory threshold (60-65% of VO2max), versus :- COPD patients trained at a similar intensity (60-65% of VO2max)- COPD patients trained at a similar absolute intensity (90% of VO2max).


Eligibility criteria: Inclusion Criteria:

* COPD patients :
* FEV1\<80%pred.
* non exacerbation during the past 4 weeks
* no oxygen therapy, -

Healthy subjects:

* \<150min/week of moderate-to-vigorous physical activity,
* Voorrips score \<9

Exclusion Criteria:

* Unstable comorbidity
* Long term oxygen therapy or ventilation
* Rehabilitation program during the past 12 months
* nutritional supplementation during the past 4 weeks
* allopurinol or N-acetylcystein during the past 4 weeks
* long corticosteroid treatment during the past 6 months
* hyperreactivity to xylocain
* anticoagulant or platelet aggregation inhibitors treatment
* participation to an other research study-pregnancy or impossibility to provide informed consent, ...

Primary outcomes:
measure: Capillary-o-fiber ratio assessed by immunochemistry on a muscle biopsy of the vastus lateralis of the quadriceps
timeFrame: 5 weeks
measure: Capillary-o-fiber ratio assessed by immunochemistry on a muscle biopsy of the vastus lateralis of the quadriceps
timeFrame: 10 weeks
Secondary outcomes:
measure: quadriceps endurance
timeFrame: 5 weeks
measure: quadriceps endurance
timeFrame: 10 weeks
measure: Pericyte coverage of skeletal muscles capillaries assessed by immunostaining of a muscle biopsy of the vastus lateralis of the quadriceps
timeFrame: 5 weeks
measure: Pericyte coverage of skeletal muscles capillaries assessed by immunostaining of a muscle biopsy of the vastus lateralis of the quadriceps
timeFrame: 10 weeks
measure: Effect of the serum of COPD patients and SHS on pericyte morphology and in vitro
timeFrame: 10 weeks
measure: Effect of the serum of COPD patients and SHS on pericyte/endothelial cells interactions in vitro
timeFrame: 10 weeks
measure: Effect of the serum of COPD patients and SHS on pericyte/endothelial cells interactions in vivo
timeFrame: 10 weeks
Other outcomes:
Locations:
city: Montpellier
country: France
facility: Department of clinical Physiology - University Hospital
Study officials:
affiliation: University Hospital
name: Farés Gouzi
role: PRINCIPAL_INVESTIGATOR
References:
citation: Souweine JS, Gouzi F, Badia E, Pomies P, Garrigue V, Morena M, Hayot M, Mercier J, Ayoub B, Quintrec ML, Raynaud F, Cristol JP. Skeletal Muscle Phenotype in Patients Undergoing Long-Term Hemodialysis Awaiting Kidney Transplantation. Clin J Am Soc Nephrol. 2021 Nov;16(11):1676-1685. doi: 10.2215/CJN.02390221.