Clinical trial NCT04719429: The Effects of Cricket- and Beef-derived Protein on Plasma Amino Acid Concentrations and Appetite Responses
Official title: The Effects of Dietary Protein Source on Postprandial Plasma Amino Acid Availability, Gut-derived Appetite Regulating Hormones, and Subjective Appetite Sensations in Young Males
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Aminoacidemia
Appetitive Behavior
Keywords: dietary protein
insect protein
young men
beef collagen
amino acids
appetite
Lead sponsor: McGill University
Sponsor class: OTHER
First posted: 2021-01-22
Enrollment: 20
Sex: MALE
Minimum age (years): 18
Maximum age (years): 35
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: CROSSOVER
Primary purpose: BASIC_SCIENCE

Brief summary: Dietary protein plays an important role in appetite regulation. Source of ingested dietary protein may have different effects on appetite, satiety, and/or food intake in humans. Insects are a rich source of protein consumed by many people around the world; however, the capacity of insect-derived protein to regulate appetite and food intake is unclear. The purpose of this study was to compare postprandial plasma glucose, insulin, amino acid, and appetite regulatory hormone concentrations, subjective appetite sensations, and food intake following the ingestion of 25 g of cricket- and beef-derived protein in healthy young males.

Detailed description: With the rapid increase in the global population, the production of sufficient amounts of conventional animal-based protein to meet global dietary demands may no longer be desired nor feasible. Insects may represent an environmentally sustainable additional source of dietary protein that has the potential to help ensure global food security in the future. However, the functional characteristics of insect-derived proteins when fed to humans is unclear. Further, how insect-derived proteins compare to other animal-derived proteins (e.g. from beef) have not been explored.

The purpose of this study was to compare postprandial plasma glucose, insulin, and amino acid concentrations, gut-derived appetite regulatory hormones, subjective appetite sensations, and ad libitum energy intake following the ingestion of 25 g insect- or beef-derived protein in healthy young men. It was hypothesized that hyperaminoacidaemia would be more rapid following the ingestion of beef-derived protein compared to cricket-derived protein, although total amino acid availability would be similar between protein sources over a 300-minute postprandial period. It was further hypothesized there would be no difference between protein sources on postprandial subjective appetite sensations or subsequent ad libitum energy intake.

Eligibility criteria: Inclusion Criteria:

* Male
* Aged between 18-35 years inclusive
* Healthy, moderately active
* BMI \< 30 kg/m2 and \> 18.5 kg/m2
* Having given informed consent

Exclusion Criteria:

* Presence of any identified metabolic or intestinal disorders
* Use of tobacco products
* Adherence to a strict vegetarian or vegan diet
* Use of medications known to affect protein metabolism
* Allergy to shellfish or crustaceans
* Allergy to beef protein
* Engagement in physical exercise training more than 6 days per week

Primary outcomes:
measure: Plasma leucine concentration
timeFrame: 0-5 hours in the post-prandial period
measure: Subjective appetite sensations (hunger, fullness, desire to eat, prospective food consumption)
timeFrame: 0-5 hours in the post-prandial period
Secondary outcomes:
measure: Plasma branched-chain amino acid concentration
timeFrame: 0-5 hours in the post-prandial period
measure: Plasma essential amino acid concentration
timeFrame: 0-5 hours in the post-prandial period
measure: Plasma non-essential amino acid concentration
timeFrame: 0-5 hours in the post-prandial period
measure: Plasma total amino acid concentration
timeFrame: 0-5 hours in the post-prandial period
measure: Plasma glucose
timeFrame: 0-5 hours in the post-prandial period
measure: Plasma insulin
timeFrame: 0-5 hours in the post-prandial period
measure: Plasma GLP-1
timeFrame: 0-5 hours in the post-prandial period
measure: Plasma PYY
timeFrame: 0-5 hours in the post-prandial period
measure: Ad libitum food energy intake
timeFrame: assessed 5 hours after protein intake
Other outcomes:
Locations:
city: Montreal
country: Canada
facility: Exercise Metabolism and Nutrition Research Laboratory
state: Quebec
Study officials:
affiliation: McGill University
name: Tyler A Churchward-Venne, Ph.D.
role: PRINCIPAL_INVESTIGATOR
References: