Clinical trial NCT03088839: Circulating Biomarker for Amyotrophic Lateral Sclerosis (ALS)
Official title: Observational Case-Control Study to Identify Circulating miR-218 as a Possible Non-invasive Biomarker of Amyotrophic Lateral Sclerosis (ALS)
Study type: OBSERVATIONAL
Overall status: UNKNOWN
Conditions: Amyotrophic Lateral Sclerosis (ALS)
Lead sponsor: Neuromed IRCCS
Sponsor class: OTHER
First posted: 2017-03-23
Enrollment: 60
Sex: ALL
Minimum age (years): 20
Maximum age (years): 75
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: yes
Observational model: CASE_CONTROL
Time perspective: CROSS_SECTIONAL
Sampling method: PROBABILITY_SAMPLE
Study population: A total of 60 individuals (30 subjects clinically diagnosed as ALS patients and 30 healthy controls) will be recruited at Centre for Rare Diseases, IRCCS Neuromed

Brief summary: Amyotrophic Lateral Sclerosis (ALS) is a rare disease with a worldwide incidence of 2-3 cases per 100,000 individuals/year and it is characterized by progressive neurodegeneration of motor neurons. When motor neurons degenerate the ability of the brain to initiate and control muscle movement is lost. ALS manifests in two forms: Familiar ALS (FALS) with inherited risk genotypes, accounts for only 10% of cases and sporadic ALS (SALS) without apparent heritability accounts for 90% of cases. ALS can occur in both female and male subjects at any age but is more common in people aged over 40.

Although the molecular mechanism underlying the pathogenesis of ALS is still under investigation, recent research has revealed that diseases affecting motor neurons may be associated to alterations of RNA metabolism and biogenesis of small non-coding micro RNAs (miRNAs). miRNAs are circulating molecules, whose expression profiles are widely described to have an important potential in monitoring the progression of a disease, to promote the development of more targeted therapies and/or to determine the effectiveness of treatments. Altered patterns of specific miRNAs expression have been described in several pathological conditions. Evidence shows a significant reduction in the levels of certain miRNAs also in patients with ALS. Among others, miRNA-218 has been described to play a critical role in the onset of motor neurons differentiation and in establishing cell identity and fate.

Changes in the levels of miRNA-218 in the serum of ALS patients may potentially provide useful tools to determine the possible association with this disease and to candidate it as indicator of disease progression.


Eligibility criteria: Inclusion Criteria:

* ALS patients admitted to Centre for Rare Diseases, IRCCS Neuromed,
* Patients diagnosed ALS within 6 months,
* Patients age between 20 years and 75 years old,
* Patients underwent to differential diagnosis using diagnostic tools (EMG, NCV, MRI) to exclude other diseases with similar signs and symptoms,
* Subjects able to communicate verbally or by using a non-verbal communication system.

Exclusion Criteria:

* Pregnant women,
* Subjects with malignant tumor,
* Subjects/Patients with others neurological or psychiatric disorders,
* Subjects/Patients with systemic diseases,
* Subjects/Patients with positive blood test for hepatitis B or C, or HIV,
* Patients included in other clinical trials,
* Subjects/Patients showing inability to understand the informed consent and the study's purpose

Primary outcomes:
measure: Identification of circulating miR-218 as biomarker for ALS
timeFrame: 8 months
Secondary outcomes:
Other outcomes:
Locations:
city: Pozzilli
country: Italy
facility: IRCCS Neuromed
Study officials:
References: