Clinical trial NCT06540755: Analysis of Pregnancy-Associated Plasma Protein-A and Interleukin-6 Gene Polymorphisms in Patients With Periodontitis
Official title: Analysis of Pregnancy-Associated Plasma Protein-A and Interleukin-6 Gene Polymorphisms in Patients With Periodontitis
Study type: OBSERVATIONAL
Overall status: COMPLETED
Conditions: Periodontitis
Polymorphism, Restriction Fragment Length
Keywords: Polymorphism
Pregnancy-Associated Plasma Protein-A
Interleukin-6
genetic
Lead sponsor: Marmara University
Sponsor class: OTHER
First posted: 2024-08-06
Enrollment: 226
Sex: ALL
Minimum age (years): 18
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: yes
Observational model: CASE_CONTROL
Time perspective: CROSS_SECTIONAL
Sampling method: PROBABILITY_SAMPLE
Study population: Group H: The periodontally healthy individuals had an absence of detectable bone and attachment loss, less than 10% of sites with bleeding on probing, and probing depths of ≤3 mm.

Group P: Stage III grade B or grade C periodontitis patients were diagnosed on the base of the 2017 World Workshop on the Classification of Periodontal and Peri-Implant Diseases and Conditions

Brief summary: The aim of this study is to evaluate the relationship between pregnancy associated plasma protein-A (PAPP-A) and interleukin-6 (IL-6) gene polymorphisms and stage 3 grade B and C (S3GBC) periodontitis, and to compare periodontitis patients with healthy individuals.

Detailed description: A total of 226 systemically healthy subjects, consisted of 112 S3GBC periodontitis patients (P group) and 114 periodontally healthy individuals (H group) were included in our study. Clinical and demographic data were recorded. Genomic deoxyribonucleic acid (DNA) molecules were obtained from peripheral blood. Genotype for IL-6 rs1800795 and PAPP-A rs7020782 polymorphisms were determined using real-time polymerase chain reaction with allele-specific probes. The genotypic or allelic distributions of PAPP-A and IL-6 gene polymorphisms were similar between groups (p\>0.05). Our finding suggests that there is no relationship between PAPP-A and IL-6 gene polymorphismS and S3GBC periodontitis, furthermore no difference was detected between periodontitis patients and healthy individuals regarding this gene polymorphism.

Eligibility criteria: Inclusion Criteria:

Participants were eligible for inclusion if they were; systemically healthy non-smokers had not undergone periodontal treatment in the past 6 months had not used antibiotics or anti-inflammatory drugs in the preceding 3 months had not used any medication that could affect periodontal tissues in the past 6 months were 18 years of age or older were not pregnant, and were not lactating.

Exclusion Criteria:

The samples taken cannot be stored under appropriate conditions and are damaged.

The onset of a systemic disease in a systemically healthy individual.

Primary outcomes:
measure: The genotypic or allelic distributions of PAPP-A and IL-6 gene polymorphisms
timeFrame: baseline
Secondary outcomes:
Other outcomes:
Locations:
city: İstanbul
country: Turkey
facility: Marmara University Dental Faculty Periodontology Departmen
Study officials:
References: