Clinical trial NCT00433173: Insulin Sensitivity in Men With the Metabolic Syndrome
Official title: Effect of Increasing Testosterone on Insulin Sensitivity in Men With the Metabolic Syndrome
Study type: INTERVENTIONAL
Overall status: SUSPENDED
Phase: NA
Conditions: Metabolic Syndrome
Keywords: testosterone
insulin sensitivity
insulin resistance
metabolic disorders
Lead sponsor: National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Sponsor class: NIH
Collaborators: name: American Diabetes Association
First posted: 2007-02-09
Enrollment: 72
Sex: MALE
Minimum age (years): 50
Maximum age (years): 75
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: no
Allocation: RANDOMIZED
Intervention model: PARALLEL
Primary purpose: TREATMENT

Brief summary: The metabolic syndrome is a medical condition defined by high levels of cholesterol in the blood, high blood pressure, central obesity (gain in fat around the region of the stomach), and insulin resistance (body responds less well to insulin). This state of impaired insulin resistance can lead to type 2 diabetes mellitus, which is one of the most common metabolic disorders in the U.S. Numerous studies have shown an inverse relationship between insulin resistance and testosterone levels in men, however, causality has not been established. This protocol investigates the role of testosterone in modulating insulin sensitivity in insulin resistant states such as the metabolic syndrome. The hypothesis is that testosterone administration will improve insulin sensitivity.

Detailed description: This protocol will address the impact of three months of testosterone (T) therapy on all components of the metabolic syndrome and the mechanism underlying changes in insulin sensitivity by analyzing changes in body composition, and detailed studies of fat metabolism and skeletal muscle. In addition, this protocol will address the role of estradiol (E2) in mediating the effect of testosterone on insulin sensitivity.

Seventy-two subjects will be enrolled. Study subjects will undergo a screening visit to assess eligibility after which a baseline metabolic assessment will be performed including a a fasting oral glucose tolerance test (OGTT) to measure normal glucose and insulin metabolism, an intravenous glucose tolerance test (IVGTT) to measure insulin sensitivity, MRI and DEXA scan to assess muscle and body fat distribution, VO2 max test and resting metabolic rate, and a muscle biopsy to look at how the muscle is affected by insulin and testosterone (T).

Subjects will then be randomized to one of three 12-week treatment arms, 1) Group 1 (Placebo); 2) Group 2 (Depot GnRH agonist (Zoladex) + Testosterone + placebo); or 3) Group 3 (Zoladex + Testosterone + aromatase inhibitor (anastrozole)). The rationale for this study design is as follows. Under normal physiological conditions, administration of T leads to a concomitant increase in estradiol (E2) levels due to endogenous conversion by the aromatase enzyme system. Therefore, in order to understand the relative roles of T and E2 on insulin sensitivity, one group of subjects will receive T in conjunction with the aromatase inhibitor, anastrozole.

At 13 weeks, the entire baseline evaluation including OGTT, IVGTT, resting metabolic rate and VO2 max, body composition assessment by DEXA and MRI, and muscle biopsy will be repeated. Subjects will return for a follow up visit four weeks later to measure CBC, T and PSA levels, to ensure levels are within the normal range.

Eligibility criteria: Inclusion Criteria:

* Age 50-75 yr
* Diagnosis of the metabolic syndrome defined by the American Heart Association/National Heart, Lung, and Blood Institute guidelines as the presence of three or more of the following:

  * Waist circumference \> 102 cm
  * Serum triglycerides \> 150 mg/dL
  * HDL cholesterol \< 40 mg/dL
  * Blood pressure \> 130 mm Hg systolic or 85 mm Hg diastolic, or treatment with anti-hypertensives
  * Fasting serum glucose \> 100 mg/dL
  * Plasma total testosterone level less than 300 ng/dL (1 SD below the mean for young healthy men)
  * Stable weight for previous three months (no weight change greater than or equal to +/-10 lbs)
  * Normal TSH, prolactin and prostate specific antigen (PSA) levels (\<2.5 ng/mL)

Exclusion Criteria:

* New diagnosis of type 2 diabetes as defined by the ADA criteria: fasting glucose greater than 126 mg/dL or random blood glucose greater than 200 mg/dL on two occasions, or on oral hypoglycemic agents
* Contraindication to stress testing
* Contraindication to MRI scanning (Central nervous system aneurysm clips; Implanted neural stimulator; Implanted cardiac pacemaker or defibrillator; Cochlear implant; Ocular foreign body (e.g. metal shavings); Insulin pump; Metal shrapnel or bullet)
* History of testicular disorders (i.e. cryptorchidism)
* History of bleeding disorders (i.e. thrombocytopenia) or baseline hemoglobin levels less than 12g/dL
* History of metabolic bone disease (osteoporosis, osteomalacia)
* History of prostate cancer
* History of sleep apnea (subjects will also be excluded if at their baseline assessment they admit to heavy snoring, restless sleep, and/or excessive daytime somnolence)
* Symptoms of urinary outflow obstruction (i.e. benign prostatic hypertrophy)
* Illicit drug use or heavy alcohol use (\>4 drinks/day)
* Allergic disorders
* Current medications (must exclude individuals taking the following medications):

  * Testosterone,
  * Cimetidine,
  * Spironolactone,
  * Ketoconazole,
  * Finasteride,
  * DHEA,
  * Androstenedione,
  * Oral steroids,
  * GnRH analogs

Primary outcomes:
measure: insulin sensitivity
timeFrame: at baseline and after 3 months
Secondary outcomes:
measure: glucose metabolism
timeFrame: at baseline and after 3 months
measure: body composition VO2 max; resting metabolic rate; muscle biopsy analysis
timeFrame: at baseline and after 3 months
measure: VO2 max
timeFrame: at baseline and after 3 months
measure: resting metabolic rate
timeFrame: at baseline and after 3 months
measure: muscle biopsy analysis
timeFrame: at baseline and after 3 months
Other outcomes:
Locations:
city: Baltimore
country: United States
facility: Johns Hopkins Bayview Medical Center
state: Maryland
city: Boston
country: United States
facility: Massachusetts General Hospital
state: Massachusetts
Study officials:
affiliation: Massachusetts General Hospital
name: Frances J Hayes, MD
role: PRINCIPAL_INVESTIGATOR
References:
citation: Caronia LM, Dwyer AA, Hayden D, Amati F, Pitteloud N, Hayes FJ. Abrupt decrease in serum testosterone levels after an oral glucose load in men: implications for screening for hypogonadism. Clin Endocrinol (Oxf). 2013 Feb;78(2):291-6. doi: 10.1111/j.1365-2265.2012.04486.x.