Clinical trial NCT03119350: Mitochondrial Energy Metabolism in Obese Women
Official title: Mitochondrial Energy Metabolism in Obese Women Undergoing Concurrent Physical Training
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Metabolism Disorder
Mitochondrial Alteration
Physical Activity
Obesity
Lead sponsor: University of Sao Paulo
Sponsor class: OTHER
First posted: 2017-04-18
Enrollment: 14
Sex: FEMALE
Minimum age (years): 20
Maximum age (years): 40
Standard ages: ADULT
Accepts healthy volunteers: no
Allocation: NA
Intervention model: SINGLE_GROUP
Primary purpose: TREATMENT

Brief summary: Considering that the failure of the treatment of obesity is justified by the multifactorial pathophysiology of this morbidity, the present project has the following hypotheses:

1. The occurrence of obesity is due to the derange,ent of mitochondrial energy metabolism ;
2. The unbalance is therapeutically modified through physical training ;
3. Obesity courses with the break-down in energy metabolism mitochondrial disease associated with systemic inflammatory characteristics that can be corrected through a combined long-term physical training program.

This study have as objective : to analyse changes in mitochondrial function, inflammatory profile, oxidative stress and energy metabolism caused by concurrent physical training in obese women.

Detailed description: Specific objectives:

Body composition by deuterium oxide; Metabolic rate of resting and oxidation of substrates by indirect calorimetry; Proinflammatory cytokines Anti-inflammatory cytokines Oxidative Stress: Malondialdehyde, Superoxide Dismutase, Glutathione-Peroxidase; Fatty acids: ceramide and palmitate; Mitochondrial respiration and citrate synthase enzyme; Quantify and qualify: mitochondrial number, endoplasmic reticulum structure, adipose cell size; Gene expression, quantify by microscopy and analyze the protein by western blot.

The study began with 20 women, however, there was withdrawal of 6, ending with 14 women.

Eligibility criteria: Inclusion Criteria:

* This study included women with obesity (BMI of 30 to 40 kg / m²), sedentary, with no associated comorbidity, convenience sample

Exclusion Criteria:

* Women who have undergone bariatric surgery, menopause, cancer or any metabolic disease, smokers, alcoholics, who are in use of drugs that act directly on the metabolism and that have medical impediment to the practice of physical exercise.

Primary outcomes:
measure: Changes Body weight
timeFrame: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
measure: Changes Body composition
timeFrame: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
measure: Changes White adipose tissue biopsy
timeFrame: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
measure: Changes Indirect calorimetry
timeFrame: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
measure: Changes in fatty acids
timeFrame: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
measure: Changes oxidative stress
timeFrame: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
measure: Changes inflammatory cytokines
timeFrame: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
measure: Changes in total cholesterol
timeFrame: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
measure: Changes Physical Performance
timeFrame: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
measure: Changes in Determination of Lactate
timeFrame: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
measure: Changes Food intake
timeFrame: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
measure: Changes Nitrogen Balance
timeFrame: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
measure: Changes Telomere length
timeFrame: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
Secondary outcomes:
Other outcomes:
Locations:
city: Ribeirao Preto
country: Brazil
facility: Camila Fernanda Cunha Brandão
state: SP
Study officials:
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