Clinical trial NCT05735392: Liquid Biopsy: Intercepting Mutational Trajectories of HER2 (Human Epidermal Growth Factor Receptor 2) Breast Cancer (GIM21 Trial)
Official title: Liquid Biopsy: Intercepting Mutational Trajectories of HER2 Breast Cancer Inpatients Under T-DM1 Treatment
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Metastatic Breast Cancer
Keywords: LiqERBcept
Trastuzumab emtansine
Liquid Biopsy
Lead sponsor: Consorzio Oncotech
Sponsor class: OTHER
First posted: 2023-02-21
Enrollment: 48
Sex: FEMALE
Minimum age (years): 18
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: no
Allocation: NA
Intervention model: SINGLE_GROUP
Primary purpose: OTHER

Brief summary: This is an open, interventional, non-pharmacological, prospective study. Patients will receive trastuzumab emtansine (T-DM1) at 3.6 mg/kg intravenously every 21 days, as per Summary of Product Characteristics (SmPC). This is a no-profit study.

Detailed description: T-DM1 effects will be monitored by a combination of Next Generation Sequencing (NGS, tumor tissue) and Liquid Biopsy (LB, blood), as described below, to capture molecular events (gene aberrations, mainly mutations) associated with (or causative of) relapse as well as primary/adaptive resistance to HER2 blockade.

This study will prospectively monitor these events throughout the clinical history of the patients (archival tissues, blood collection, aimed biopsies). No investigational drugs will be administered.

This protocol is classified as "interventional" for two reasons:

1. dedicated, additional blood drawings (liquid biopsies)are collected in addition to the routine bloodtests being requested as per standard clinical practice;
2. the study involves occasional fine-needle biopsy on accessible (e.g. cutaneous) metastatic foci in selected patients to confirm their HER2 status, as per good medical practice.

These will represent an additional opportunity of targeted NGS.

Both blood (a) and tissues (b) will be obtained during T-DM1 treatment of enrolled patients at the participating Sites at the study specific timelines.

Eligibility criteria: Inclusion criteria

1. Male and female patients with a documented diagnosis of metastatic HER2-positive breast cancer (BC) as defined by an immunohistochemistry (IHC) score of 3+, alternatively score 2+ and HER2 amplification ratio ≥ 2.0
2. All patients eligible to treatment with T-DM1, according to SmPC previously treated with a taxane and trastuzumab. Patients who previously underwent first-line treatment with an association of TTZ with PTZ are also eligible. No more than one line of anti-HER2 treatment for advanced disease are allowed.
3. Available tissue from the primary tumor. If possible, newly obtained core or excisional biopsy on metastatic site at baseline (this biopsy won't impact on timing for patient enrollment and not leading to patient exclusion). High quality genomic DNA from the above for NGS mutational analysis.
4. Patients with both measurable and non-measurable disease (according to modified RECIST 1.1 criteria) are eligible.
5. 18 years of age on day of signing informed consent.
6. a left ventricular ejection fraction of 50% or more (determined by echocardiography or multiple-gated acquisition \[MUGA\] scanning);
7. an Eastern Cooperative Oncology Group performance status of 0 or 1.
8. Adequate organ function (obtained within 14 days prior to treatment study) as evidenced by:

   1. Absolute neutrophil count (ANC) 1.5 X 109/L without myeloid growth factor support for 7 days preceding the lab assessment;
   2. Haemoglobin (Hgb) 9 g/dL (90 g/L); \< 9 g/dL (\< 90 g/L) is acceptable if hemoglobin is corrected to 9 g/dL (90 g/L) ;
   3. Platelet count 75 X 109/L without blood transfusions for 7 days preceding the lab assessment;
   4. Bilirubin 1.5 X upper limit of normal (ULN), except for patients with a documented history of Gilbert's disease;
   5. Alanine aminotransferase (ALT), and aspartate aminotransferase (AST) 2.5 X ULN (for patients with liver metastases 5 X ULN);
9. Life expectancy \> 12 weeks;
10. Written informed consent obtained before any screening procedure and according to local guidelines.

Exclusion criteria

1. Prior treatment with T-DM1.
2. Symptomatic central nervous system (CNS) metastases, or treatment for these metastases within the 2 months preceding enrollment.
3. Current participation in study therapy, or previous participation in a study involving the administration of an investigational agent within 4 weeks of administration of the first dose of treatment.
4. History of symptomatic congestive heart failure or serious cardiac arrhythmia requiring treatment; a history of myocardial infarction or unstable angina within 6 months before.
5. Female patients who are pregnant or lactating, who plan to get pregnant, or who have a positive serum pregnancy test prior to first dose of study treatment.
6. Prior malignancy (other than breast cancer) except for non-melanoma skin cancer and carcinoma in situ (of the cervix or bladder), unless diagnosed and definitively treated more than 5 years prior to enrollment.
7. Any other significant co-morbid conditions that in the opinion of the Investigator would impair study participation or cooperation.
8. Patients with psychiatric illness, social situation or geographical situation that would preclude informed consent or limit compliance with study requirements, as determined by the Investigator.

Primary outcomes:
measure: Number of index mutations
timeFrame: About 4 years
measure: Rate of response/anticipation of relapse
timeFrame: About 4 years
measure: Number of de novo mutations
timeFrame: About 4 years
Secondary outcomes:
measure: Number of actionable mutations
timeFrame: About 4 years
Other outcomes:
Locations:
city: Bergamo
country: Italy
facility: A.O. Ospedale Papa Giovanni XXIII - Oncologia
city: Genova
country: Italy
facility: I.R.C.C.S. A.O.U San Martino - IST
city: Modena
country: Italy
facility: A.O.U. Policlinico di Modena
city: Napoli
country: Italy
facility: Azienda Ospedaliera Universitaria Federico II
city: Negrar
country: Italy
facility: Ospedale S. Cuore Don Calabria
city: Roma
country: Italy
facility: Fondazione Policlinico Universitario A. Gemelli - Oncologia Medica
city: Roma
country: Italy
facility: Fondazione Policlinico Universitario A. Gemelli - Senologia Oncologica
city: Roma
country: Italy
facility: Istituto Nazionale Tumori "Regina Elena"
city: Rome
country: Italy
facility: Policlinico Umberto I
Study officials:
affiliation: Clinical issues - Fondazione Policlinico Gemelli
name: Alessandra Fabi, MD
role: PRINCIPAL_INVESTIGATOR
affiliation: Central laboratory assesment and liquid biopsy - Oncogenomics and Epigenetics - Istituto Nazionale Tumori "Regina Elena"
name: Patrizio Giacomini
role: PRINCIPAL_INVESTIGATOR
affiliation: Chairman - Istituto Nazionale Tumori "Regina Elena"
name: Francesco Cognetti
role: STUDY_CHAIR
References: