Clinical trial NCT04877262: Effects of Time-restricted Eating on Nutrient Absorption in Healthy Adults
Official title: Effects of Time-restricted Eating on Nutrient Absorption in Healthy Adults
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Digestibility
Keywords: stool energy loss
digestibility
intestinal absorption
overweight
microbiome
metabolomics
energy balance
Lead sponsor: Florida State University
Sponsor class: OTHER
First posted: 2021-05-07
Enrollment: 17
Sex: ALL
Minimum age (years): 20
Maximum age (years): 45
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: CROSSOVER
Intervention model description: The study is a randomized, crossover, controlled feeding study design consisting of two 9-d weight maintenance diet periods separated by at least 3 weeks.
Primary purpose: BASIC_SCIENCE

Brief summary: This 2-period, crossover, controlled-feeding trial will investigate the effects of early time-restricted eating (8am-2pm), compared to a control eating timeframe (8am-8pm), on energy and macronutrient digestibility, the thermic effect of food (TEF), postprandial blood metabolites, 24 h glucose concentrations, glycemic variability, intestinal hydrogen gas production, gastrointestinal transit time, and microbiome composition. This study will also seek to determine factors that may predict intestinal absorption efficiency (i.e., digestibility) including the microbial composition of stool, fasting and postprandial metabolomics, gastrointestinal transit time, thermic effect of food, 24 h glucose concentrations and variability, and hydrogen gas production.

Detailed description: The obesity epidemic is well documented, and despite a complex etiology influenced by genetic, physiological, psychological, social, behavioral, and environmental factors, obesity seems to be the result of energy imbalance, where energy intake exceeds energy expenditure, regardless of the internal and external influences. Studies aiming to quantify and manipulate modulators of energy balance, including gastrointestinal (GI) energy absorption rates (i.e., digestibility), are crucial for reducing the incidence of overweight and obesity and improving overall human health. Despite considerable interindividual variability in the amount of ingested energy that is absorbed (\~87-98% of ingested energy) by the gastrointestinal tract, generalized equations are used to calculate individual energy intake requirements, and the factors that contribute to such variability, including meal-timing, are largely unknown.

The proposed study seeks to determine the effects of restricting the eating window to 6-h (early time-restricted eating) as compared to a control eating window of 12 h on energy and macronutrient digestibility, thermic effect of food (TEF), postprandial blood metabolites (triglyceride, insulin, glucose, fructose, galactose, inositol, and sorbitol), 24 h glucose concentrations and glycemic variability, intestinal hydrogen gas production, microbiome composition, and gastrointestinal transit time in response to a controlled, energy balanced diet. In addition, this project will determine whether energy digestibility can be predicted from biological and physiological factors such as gut microbiota composition, gastrointestinal transit time, 24-h glucose concentrations, and postabsorptive and postprandial metabolomic profiles.

Participants will be randomized to early TRE (6-h feeding window, 8am-2pm) or a control eating schedule (12-h feeding window, 8am-8pm) and consume a controlled, energy balance diet (breakfast, lunch, dinner, snacks, and beverages) for 9 consecutive days before crossing over to the other intervention after at least a 3-wk washout for females (to control for hormonal fluctuations) and at least a 2-wk washout for males. Participants will collect stool and urine during each feeding phase. Gross energy content (heat combustion by bomb calorimetry) of the diet, stool, and urine samples will be used to calculate energy digestibility (diet gross energy - stool gross energy) and metabolizable energy. Factors that may mediate and/or predict energy digestibility efficiency, including gastrointestinal microbial composition, transit time, and gas production, fasting and postprandial metabolites, continuous glucose concentrations, and thermogenesis will be assessed.

Eligibility criteria: Inclusion Criteria:

* 20-45 years old
* Normal weight or overweight (18.5 to 29.9 kg/m2)
* Not taking any probiotics for 4 weeks prior to and throughout the entire study
* Willing to refrain from smoking, vaping, chewing tobacco, and dietary supplement use throughout the study
* Willing to consume a controlled diet for 9 consecutive days on 2 different occasions (18 days total) and collect all urine and fecal output for 3-5 days during each occasion
* Pass the blue dye gastrointestinal tracer (given at screening) within 72 hours of administration

Exclusion Criteria:

* Metabolic or cardiovascular abnormalities, gastrointestinal disorders, prior bariatric surgery, malabsorption, or any condition that interferes with metabolism (e.g., kidney disease, diabetes, cardiovascular disease, etc.)
* Suspected or known strictures, fistulas, or physiological/mechanical gastrointestinal obstruction
* Present condition of alcoholism, anabolic steroids, or other substance abuse issues
* Women who are pregnant, lactating, planning to become pregnant, or who have an irregular menstrual cycle in the past 6 months
* Weight fluctuations ± 5% of body weight in the last 6 months
* Allergies or intolerance to foods included in the controlled diet
* Any use of antibiotics, except topical antibiotics, within 3 months of study participation
* Colonoscopy within 3 months of study participation
* Use of laxatives, stool softeners, or anti-diarrheal medications more than once a week within 4-weeks of study enrollment

Primary outcomes:
measure: Energy digestibility
timeFrame: Days 4-9
Secondary outcomes:
measure: Fat digestibility
timeFrame: Days 4-9
measure: Carbohydrate digestibility
timeFrame: Days 4-9
measure: Protein digestibility
timeFrame: Days 4-9
measure: Metabolizable energy
timeFrame: Days 4-9
measure: Metabolizable protein
timeFrame: Days 4-9
measure: Postabsorptive and postprandial plasma metabolites
timeFrame: Day 6
measure: Gut microbiome
timeFrame: Day 6
measure: Gastrointestinal transit time
timeFrame: Day 6
measure: Gastrointestinal gas production
timeFrame: Day 6
measure: Gastrointestinal temperature
timeFrame: Day 6
measure: 24-h glucose concentrations
timeFrame: Days 1-8
measure: 24-h glucose variability
timeFrame: Days 1-8
measure: Thermic effect of food
timeFrame: Day 6
Other outcomes:
Locations:
city: Tallahassee
country: United States
facility: Florida State University
state: Florida
Study officials:
affiliation: Florida State University
name: Claire E Berryman, Ph.D.
role: PRINCIPAL_INVESTIGATOR
References: