Clinical trial NCT06566495: Microorganisms on Reusable Tourniquets
Official title: Reusable Tourniquets as Potential Risk of Microbial Transmission - Comparison of Operating Theatre and Emergency Department
Study type: OBSERVATIONAL
Overall status: ACTIVE_NOT_RECRUITING
Conditions: Microbial Colonization
Infection, Bacterial
HAI
Nurse's Role
Vascular Access Device Complications
Vascular Access Complication
CRBSI - Catheter Related Bloodstream Infection
Catheter Infection
Device Colonisation
Keywords: Tourniquet
Cross Infection
Vascular Access Devices
Infection Control
Lead sponsor: Medical University of Gdansk
Sponsor class: OTHER
First posted: 2024-08-22
Enrollment: 53
Sex: ALL
Standard ages: CHILD
ADULT
OLDER_ADULT
Observational model: OTHER
Time perspective: CROSS_SECTIONAL
Sampling method: PROBABILITY_SAMPLE
Study population: 53 reusable tourniquets

Brief summary: The purpose of this study was to compare microbial contamination on the surface of reusable stasis after indefinite use, 2 weeks and 4 weeks. We investigated how the site - the operating theater and the emergency department, as well as the time of use - affects the number of organisms.

Detailed description: The cross-sectional study was conducted in the operating theater and emergency department of a tertiary referral hospital in Gdansk, Poland, in three part from March to April 2024. The study included reusable tourniquets used by the hospital's medical staff during vascular access generation.

After each stage of the in-hospital study, the stasis was collected and replaced with new ones. A total of 53 reusable stasis were collected in three phases of the study and were subjected to microbiological analysis at the Department of Immunobiology and Environmental Microbiology of the Medical University of Gdansk. The tourniquets were collected into disposable, sterile bags. In the first stage of the study, tourniquets were collected of indefinite use (n= 17). In the second and third stages of the study, stasis used for 14 (n=20) and 28 (n=16) days, respectively, were collected. All tourniquets were labeled and assigned to different rooms located within the surveyed wards. The trial was conducted separately, for the plastic fastener and the fabric band. The plastic parts of the tourniquets were placed in sterile glass dishes. Under laboratory conditions, the plastic parts of the stasis were cut off and placed in the dishes. Using sterile swabs soaked in 0.9% sodium chloride, the plastic was swabbed, after which the tip of the swab was cut off, placed in a tube with 0.9% sodium chloride (3ml), which was shaken in a Vortex device (30 seconds) and the obtained material was seeded on columbia Agar with 5% sheep blood. The material part of the stasis was placed in a sterile homogenization bag with the addition of 100ml of nutrient broth. The bag was then placed in a Stomacher Homogenizer for two minutes to detach the microorganisms from the porous surface of the material. The resulting homogenate was transferred in a concentrated state and diluted tenfold using 200 μl pipettes onto columbia Agar media supplemented with 5% sheep blood.200 μl of concentrated homogenate was seeded onto the quality growth media MacConkey Broth, King B Agar, CHROMagar E. coli and other coliforms. The protected material was incubated for 24 hours at 37°C, in an aerobic atmosphere. After this time, bacterial colonies were counted and counts were performed to determine the number of microorganisms on the surface of the stasis (CFU/cm2). Due to the assumptions of the experiment and the time frame, eight tourniquets were not included in the analysis. From the information obtained from the medical staff, this was due to significant soiling, making it impossible to use the tourniquets, and in a few cases the plastic fastener broke.

Eligibility criteria: Inclusion Criteria:

* reusable tourniquets used to make the vessel visible

Exclusion Criteria:

* disposable tourniquets used to make the vessel visible

Primary outcomes:
measure: Standard Deviation and median of colony forming units (CFU/cm2) in the emergency department
timeFrame: 24 hours
measure: Standard Deviation and median of colony forming units (CFU/cm2) in the operating theatre
timeFrame: 24 hours
Secondary outcomes:
measure: Blood stains and CFU
timeFrame: 2 months
Other outcomes:
Locations:
city: Gdańsk
country: Poland
facility: Medical Univeristy of Gdańsk
state: Dębinki Street
Study officials:
References: