Clinical trial NCT05113914: The Age-related Changes of Energy Metabolism in Physically Fit Adult Males
Official title: The Influence of Age and Physical Fitness Level on the Level of Energy Metabolism and Antioxidant Capacity in Adult Males
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Healthy
Keywords: athletes
masters
purine metabolites
NAD metabolites
CPET
Lead sponsor: Poznan University of Physical Education
Sponsor class: OTHER
First posted: 2021-11-09
Enrollment: 420
Sex: MALE
Minimum age (years): 18
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: yes
Allocation: NA
Intervention model: SINGLE_GROUP
Primary purpose: BASIC_SCIENCE

Brief summary: The influence of age and physical fitness level on the level of energy metabolism and antioxidant capacity in adult males.

Detailed description: Over four hundred healthy, adult males of different age categories and physical fitness levels were included in the study. There is much research convincing that in athletes of various sports disciplines the level of energy metabolism, oxidative stress, and amino acid profiles during years of training undergoes sports adaptation. The study aimed to evaluate how age and physical fitness level affects the level of purine metabolites and metabolites of nicotinamide adenine dinucleotide, the level of antioxidant capacity, and amino acid profiles. Investigators hypothesized that there will be an age-related decline in most of the physiological indices, but significant differences will appear regarding the level of an individual's sports adaptation.

Eligibility criteria: Inclusion Criteria:

1. healthy
2. non-smoking
3. physically fit, including top-level athletes

Exclusion Criteria:

1. no reported history of a cardiopulmonary or renal diseases, and other chronic diseases
2. no major orthopedic injury or illness resulting in an inability to run
3. no medications that could affect cardiopulmonary functions
4. normal resting electrocardiogram
5. body mass index (BMI) below 30.0 kg·m-2
6. normal erythrocyte count and Hb content
7. no pathological states known of significantly elevated adenylate pool and concentration of ATP, e.g. sickle cell disease, diabetes, leukemia, sepsis, tuberculosis, meningococcal infection, or renal insufficiency

Primary outcomes:
measure: Cardio-respiratory measures
timeFrame: during the intervention
Secondary outcomes:
measure: Blood Lactate level
timeFrame: baseline and immediately after the intervention
measure: Plasma purine nucleotides
timeFrame: baseline and immediately after the intervention
measure: Erythrocyte Purine Metabolites
timeFrame: baseline and immediately after the intervention
measure: Hypoxanthine-guanine phosphoribosyltransferase (HGPRT) activity
timeFrame: baseline and immediately after the intervention
measure: Nicotinamide Adenine Dinucleotide (NAD) and its metabolites
timeFrame: baseline and immediately after the intervention
measure: Total antioxidant status (TAS)
timeFrame: baseline
measure: Thiobarbituric-acid-reactive substances (TBARS)
timeFrame: baseline
measure: Malondialdehyde (MDA)
timeFrame: baseline
measure: Polyphenols
timeFrame: baseline
measure: Reduced glutathione (GSH)
timeFrame: baseline
measure: Glutathione peroxidase (GPx) activity
timeFrame: baseline
measure: Aminoacid profile
timeFrame: baseline
Other outcomes:
Locations:
city: Poznań
country: Poland
facility: Poznan University of Physical Education
Study officials:
affiliation: Poznan University of Physical Education
name: Jacek Zieliński, Prof.
role: PRINCIPAL_INVESTIGATOR
References: