Clinical trial NCT06275438: Comparison of Oxytocin Receptor Immunoreactivity in Placentas Obtained From Women Having Cesarean and Natural Birth
Official title: Comparison of Oxytocin Receptor Immunoreactivity in Placentas Obtained From Women Having Cesarean and Natural Birth
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Healthy Mothers Who Have Had Both Vaginal and Cesarean Deliveries
Keywords: Cesarean Section, Decidua, Midwife, Oxytocin Receptor, Placenta
Lead sponsor: Kafkas University
Sponsor class: OTHER
First posted: 2024-02-23
Enrollment: 32
Sex: FEMALE
Minimum age (years): 18
Maximum age (years): 35
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: PARALLEL
Intervention model description: "Our study was conducted on the placentas of 40 women admitted to the obstetrics clinics of Kars Harakani State Hospital and Kafkas University Training and Research Hospital, after obtaining necessary permissions. Out of 40 placentas, 5 were excluded due to blood incompatibility, 2 were excluded due to subsequent diagnosis of chronic conditions (thyroid disorder, Behçet's disease), and 1 was excluded due to the presence of an accessory placenta, resulting in a total of 32 placentas included in the study. Among these, 16 were from women who had undergone normal vaginal delivery (control group), and 16 were from women who had undergone cesarean section (case group). Tissue samples obtained from the placentas were subjected to histological and immunohistochemical methods to obtain various findings
Primary purpose: HEALTH_SERVICES_RESEARCH

Brief summary: Our study examines the effects of oxytocin hormone receptors. mechanisms of placental tissue cells and therefore intrauterine development. It was conducted to investigate the effects on newborn babies apgar score, fontanel openings, birth weights, etc. By collecting data, normal and They were compared between cesarean births by statistical analysis. normal in this regard Based on the hypothesis that there is a difference between labor and cesarean delivery, this study has been made.OXTR primary antibody was used for immunohistochemical examination. There was no significant difference between the groups in terms of histological examination. In the study, it was found that OXTR immunoreactivity was strong in decidual cells and stromal cells in villi. A stronger OXTR immunoreactivity was detected in villous stroma of placenta of the natural birth group compared to the cesarean birth group. Reaction was not observed in syncytotrophoblast cells and syncytial nodes in both groups, but strong immunoreaction was observed fetal endothelial cells and fetal capillaries in both groups.

Detailed description: The aim of this study was to compare oxytocin receptor (OXTR) immunoreactivity in placentas obtained from women having cesarean and natural birth. The study samples consisted of placentas of 32 mothers who had 16 natural birth (control) and 16 cesarean (case) birth in Kars Harakani State Hospital and Kafkas University Health Research and Application Hospital. At the beginning of the study, 40 placentas obtained in total, 5 were not included in the study due to mismatched blood, 2 were not included in the study after the chronic disease (thyroid, Behçet's disease) detected, and 1 was not included in the study due to the presence of accessory placenta. The means of binary variables were determined by applying the Independent Two-Sample T-Test. Pearson Correlation analysis was used to examine the relationship between the variables. Periodic acid-Schiff staining, H\&E and Triple staining techniques were applied to tissue samples obtained from placentas for histological examination. OXTR primary antibody was used for immunohistochemical examination. There was no significant difference between the groups in terms of histological examination. In the study, it was found that OXTR immunoreactivity was strong in decidual cells and stromal cells in villi. A stronger OXTR immunoreactivity was detected in villous stroma of placenta of the natural birth group compared to the cesarean birth group. Reaction was not observed in syncytotrophoblast cells and syncytial nodes in both groups, but strong immunoreaction was observed fetal endothelial cells and fetal capillaries in both groups. As a result, it is thought that the placenta pathology should be made routine for every birth because it gives information about the offspring.

Eligibility criteria: Inclusion Criteria:

Mothers with a body mass index within this range:18kg/m2≥Body Mass Index (BMI)≤35kg/m2 Healthy mothers who have had both vaginal and cesarean deliveries Mothers aged over 18 and under 35 years old

Exclusion Criteria:

* Chronic diseases Gynecological disorders Intrauterine growth restriction Placental anomalies Forensic cases of pregnancy Premature births Eclampsia-Preeclampsia Multiple pregnancies Body Mass Index (BMI) ≥35kg/m2 Body Mass Index (BMI) ≤18kg/m2 Experience of trauma during pregnancy Non-cesarean surgical interventions during pregnancy Severe infections

Primary outcomes:
measure: Oksitosin Receptor Immunoreactivity
timeFrame: 2 weeks
Secondary outcomes:
measure: Pearson correlation analysis
timeFrame: 1 months
Other outcomes:
Locations:
city: Kars
country: Turkey
facility: Kafkas Universty
state: Merkez
Study officials:
affiliation: Kafkas University Faculty of Medicine
name: Seyit Ali Bingöl, associate professor dr.
role: STUDY_DIRECTOR
affiliation: Kafkas University Faculty Of Health Sciences
name: SEVİN Polat, resarch assistant
role: STUDY_CHAIR
References:
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citation: Eriksson JG, Gelow J, Thornburg KL, Osmond C, Laakso M, Uusitupa M, Lindi V, Kajantie E, Barker DJ. Long-term effects of placental growth on overweight and body composition. Int J Pediatr. 2012;2012:324185. doi: 10.1155/2012/324185. Epub 2012 Apr 18.