Clinical trial NCT03410576: Perioperative Time Course of MMP-9 and Its Inhibitor During Carotid Artery Stenting and Carotid Endarterectomy
Official title: Comparison of the Perioperative Time Course of Matrix Metalloproteinase-9 (MMP-9) and Its Inhibitor (TIMP-1) During Carotid Artery Stenting (CAS) and Carotid Endarterectomy (CEA)
Study type: OBSERVATIONAL
Overall status: COMPLETED
Conditions: Carotid Stenosis
Ischemia Reperfusion Injury
Keywords: Matrix metalloproteinase
Carotid artery stenting
Carotid endarterectomy
Lead sponsor: University of Pecs
Sponsor class: OTHER
First posted: 2018-01-25
Enrollment: 25
Sex: ALL
Minimum age (years): 18
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: no
Observational model: COHORT
Time perspective: PROSPECTIVE
Sampling method: PROBABILITY_SAMPLE
Study population: Diagnosed carotid artery stenosis

Brief summary: The aim was the comparison of the perioperative time courses of matrix metalloproteinase-9 (MMP-9) and its inhibitor (TIMP-1) during elective carotid artery stenting (CAS). The investigators used a matched, historical carotid endarteriectomy group as controls. Blood samples at four time points: T1: preoperative; T2: 60 minutes after stent insertion; T3: first postoperative morning; and T4: third postoperative morning. Plasma was isolated from heparin anticoagulated blood samples by low speed centrifugation at 4 °C, and stored at -80 °C until analyzed in a single batch at the end of the study. Plasma concentrations of MMP-9 and TIMP-1 were expressed as ng/ml.


Eligibility criteria: Inclusion Criteria:

elective carotid artery surgery

Exclusion Criteria:

Malignant diseases, inflammatory and systemic autoimmune disorders, psychiatric disorders and previous debilitating stroke

Primary outcomes:
measure: Plasma MMP-9 concentrations
timeFrame: 4 days
measure: Plasma TIMP-1 concentrations
timeFrame: 4 days
Secondary outcomes:
measure: MMP-9/TIMP-1 ratio
timeFrame: 4 days
Other outcomes:
Locations:
city: Pécs
country: Hungary
facility: Department of Anaesthesiology and Intensive Therapy, Medical School, University of Pécs
state: Baranya
Study officials:
affiliation: Department of Anaesthesiology and Intensive Therapy, Medical School, University of Pécs
name: Gábor Woth, MD, PhD
role: PRINCIPAL_INVESTIGATOR
affiliation: Department of Anaesthesiology and Intensive Therapy, Medical School, University of Pécs
name: Bálint Nagy, MD, PhD
role: PRINCIPAL_INVESTIGATOR
affiliation: Department of Anaesthesiology and Intensive Therapy, Medical School, University of Pécs
name: Diana Mühl, MD, PhD
role: STUDY_DIRECTOR
affiliation: Department of Surgical Research and Techniques, Medical School, University of Pécs,
name: János Lantos, PhD
role: PRINCIPAL_INVESTIGATOR
affiliation: Department of Neurosurgery, Medical School, University of Pécs,
name: Ferenc Kövér, MD
role: PRINCIPAL_INVESTIGATOR
affiliation: Department of Anaesthesiology and Intensive Therapy, Medical School, University of Pécs
name: Lajos Bogár, MD, DsC
role: PRINCIPAL_INVESTIGATOR
References:
citation: Nagy B, Woth G, Merei A, Nagy L, Lantos J, Menyhei G, Bogar L, Muhl D. Perioperative time course of matrix metalloproteinase-9 (MMP-9), its tissue inhibitor TIMP-1 & S100B protein in carotid surgery. Indian J Med Res. 2016 Feb;143(2):220-6. doi: 10.4103/0971-5916.180212.
citation: Merei A, Nagy B, Woth G, Lantos J, Kover F, Bogar L, Muhl D. Comparison of the perioperative time courses of matrix metalloproteinase-9 (MMP-9) and its inhibitor (TIMP-1) during carotid artery stenting (CAS) and carotid endarterectomy (CEA). BMC Neurol. 2018 Aug 29;18(1):128. doi: 10.1186/s12883-018-1133-1.