Clinical trial NCT03813082: Age-related Changes in Sleep-wake Regulation
Official title: Age-related Changes in Sleep-wake Regulation: Effects of Sleep Loss on Possible Molecular Markers of Sleep Need
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Sleep Deprivation
Keywords: Positron emission tomography
Magnetic resonance spectroscopy
Aging
Lead sponsor: University of Zurich
Sponsor class: OTHER
First posted: 2019-01-23
Enrollment: 29
Sex: MALE
Minimum age (years): 20
Maximum age (years): 70
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: yes
Allocation: NON_RANDOMIZED
Intervention model: PARALLEL
Primary purpose: BASIC_SCIENCE

Brief summary: A sleep deprivation protocol combined with state-of-the-art, simultaneous positron emission tomography and magnetic resonance spectroscopy imaging will be employed to investigate the effects of sleep deprivation and aging on hypothesized molecular markers of sleep need.


Eligibility criteria: Inclusion Criteria:

* healthy male individuals
* age: 20-35 or 60-70 years
* right-handed
* non-smokers
* moderate caffeine/alcohol consumption
* normal BMI

Exclusion Criteria:

* Sleep/circadian rhythms disturbances
* former brain injuries with loss of consciousness and brain anomalies
* drug intake/consumption
* medication intake
* cardiac pacemaker
* neurological disorders
* prior participation (during past 2 years) in radiological or nuclear experiment

Primary outcomes:
measure: Sleep-wake induced change in cerebral availability of mGluR5 (metabotropic glutamate receptors of subtype 5)
timeFrame: Change from baseline metabotropic glutamate receptors of subtype 5 availability after 33 hours of prolonged wakefulness and 8 hours of recovery sleep
Secondary outcomes:
measure: Sleep-wake induced change in glutamate concentration in prefrontal cortex and basal ganglia
timeFrame: Change from baseline glutamate concentration after 33 hours of prolonged wakefulness and 8 hours of recovery sleep
measure: Sleep-wake induced change in potential molecular markers of sleep need
timeFrame: Change from baseline microRNA, fragile X mental retardation protein and brain-derived neurotrophic factor concentrations after 33 hours of prolonged wakefulness and 8 hours of recovery sleep
measure: Sleep-wake induced change in sleep electroencephalogram (EEG)
timeFrame: Change from all-night baseline sleep EEG in 8 hours of recovery sleep after 33 hours of prolonged wakefulness
measure: Sleep-wake induced change in waking electroencephalogram (EEG)
timeFrame: Change from baseline waking EEG during 33 hours of prolonged wakefulness and after 8 hours of recovery sleep
measure: Sleep-wake induced change in subjective state
timeFrame: Change from baseline sleepiness during 33 hours of prolonged wakefulness and after 8 hours of recovery sleep
measure: Sleep-wake induced change in cognitive performance
timeFrame: Change from baseline cognitive performance during 33 hours of prolonged wakefulness and after 8 hours of recovery sleep
Other outcomes:
Locations:
Study officials:
affiliation: University of Zurich, Institute of Pharmacology and Toxicology
name: Hanspeter Landolt, Prof
role: PRINCIPAL_INVESTIGATOR
References:
citation: Weigend S, Holst SC, Treyer V, O'Gorman Tuura RL, Meier J, Ametamey SM, Buck A, Landolt HP. Dynamic changes in cerebral and peripheral markers of glutamatergic signaling across the human sleep-wake cycle. Sleep. 2019 Oct 21;42(11):zsz161. doi: 10.1093/sleep/zsz161.