Clinical trial NCT02692976: Natural Dendritic Cells for Immunotherapy of Chemo-naive Metastatic Castration-resistant Prostate Cancer Patients
Official title: A Randomized Phase IIa Study: Natural Dendritic Cells for Immunotherapy of Chemo-naive Metastatic Castration-resistant Prostate Cancer Patients
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: PHASE2
Conditions: Prostatic Neoplasms
Immunotherapy
Dendritic Cells
Vaccines
Keywords: Castration-resistant prostate cancer
Dendritic Cells
Immunotherapy
Vaccines
Lead sponsor: Radboud University Medical Center
Sponsor class: OTHER
First posted: 2016-02-26
Enrollment: 21
Sex: MALE
Minimum age (years): 18
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: no
Allocation: RANDOMIZED
Intervention model: PARALLEL
Primary purpose: TREATMENT

Brief summary: Prostate cancer is the only type of cancer in which conventional dendritic cells (DC) treatment has a beneficial effect on the overall survival. In this study investigators aim to show immunologic efficacy of tumor-peptide loaded natural DC in metastatic castration-resistant prostate cancer patients (mCRPC).

The immunomonitoring will include:

1. functional response and tetramer analysis of delayed-type hypersensitivity infiltrating lymphocytes against tumor peptides and
2. type I interferon (IFN) gene expression in peripheral blood mononuclear cells, and
3. proliferative, effector cytokine- and humoral responses to keyhole limpet hemocyanin, a immunogenic protein providing T cell help.

The secondary objectives are the safety and feasibility of natural DC vaccinations, the influence on the quality of life during treatment with natural DC, and the clinical efficacy of treatment.

Detailed description: Immunotherapy with DC vaccines Prevention of infectious diseases through immunization is one of the greatest achievements of modern medicine. Nonetheless, considerable challenges remain for improving the efficacy of existing vaccines for therapeutic immunizations for diseases such as cancer. More than 10 years ago the first groups introduced tumor antigen-loaded DC-based vaccines in the clinic. Effective immune responses and favorable clinical outcomes have indeed been observed. Thus far, mainly conventional in vitro generated monocyte-derived DCs (moDC) have been used in clinical trials worldwide. Long lasting tumor specific T cell-mediated immunological responses are clearly linked to increased progression free survival as well as overall survival.

However, moDC may not be the optimal source of DCs for DC vaccination studies, due to extensive culture periods and compounds required to obtain mature moDC. Two principal subsets of human blood DC, called plasmacytoid DC (pDC) and myeloid DC (mDC), are possibly a better alternative since they do not require extensive culture periods and are directly isolable from the peripheral-blood. Based on promising immunological and clinical outcome with pDC and mDC vaccinations in metastatic melanoma patients, further testing of these blood DC subsets is warranted. Based on these observations investigators are convinced that pDC and mDC employ different, and probably more optimal mechanisms to combat cancer. In addition, based on in vitro data and preclinical studies that suggest that natural DC act synergistically, investigators hypothesize that the combination of pDC and mDC may induce stronger anti-tumor immune responses as compared to pDC or mDC alone.

Immunotherapy in prostate cancer Prostate cancer is the most common noncutaneous cancer in men. In recent years novel therapies have been studied extensively. Prostate cancer is usually diagnosed in men above 65 years of age. Depending on the severity of the disease, current treatment options for prostate cancer consist of active surveillance, prostatectomy, radiation therapy, hormonal therapy, or chemotherapy. Up to one-third of patients with a localized tumor eventually fails on local therapy and progress to advanced-stage or metastatic disease within 10 years. Although the majority of patients initially respond to anti-androgens, most tumors become resistant within 14 to 30 months. For men with mCRPC the median survival in phase III studies range from 15 to 19 months. The chemotherapeutic drug docetaxel was for several years the only treatment option for mCRPC, resulting in a median overall survival benefit of two to three months compared to mitoxantrone. In the past five years second-line chemotherapy (cabazitaxel), second-generation androgen deprivation therapy (abiraterone acetate plus prednisone and enzalutamide), cellular immunotherapy (sipuleucel-T), and a targeted alpha emitter (radium-223 treatment) have expanded the treatment repertoire for mCRPC.

Sipuleucel-T, a DC-based vaccine for patients suffering from prostate cancer, has shown to be clinically effective and is approved by the Food and Drug Administration and European Medicines Agency for mCRPC patients. A major advantage of cellular immunotherapy when compared to chemotherapy, and even androgen deprivation therapy, is its low toxicity. Several other immunotherapeutic approached have been investigated and potential tumor antigens have been identified. Prostvac (vaccinia-prostate-specific antigen) was administered in a randomized phase II study with encouraging results. Ipilimumab had promising results in several phase II studies and in combination with a vaccine, such as GVAX. However, in a phase III study post-chemotherapy trial ipilimumab seemed not superior to placebo. Hence, only sipuleucel-T had clinical significant results in clinical trials.

The promising immunological and clinical outcome with pDC and mDC in metastatic melanoma warrants further testing of these blood DC in prostate cancer. In this study investigators aim to show proof-of-principle of natural DC immunogenicity in prostate cancer patients: induction/enhancement of tumor-specific T cells by mDC and induction of an IFN signature by pDC. Investigators will also get insight if combining subsets improves immunogenicity and clinical outcome. Hence, there is an urgent need for a potent treatment modality together with a solid predictive and prognostic biomarker.

Eligibility criteria: Inclusion Criteria:

* Men ≥ 18 years of age and older with confirmed (histologically or cytologically) adenocarcinoma of the prostate without neuroendocrine differentiation or small cell features
* Human leukocyte antigen (HLA)-A2.1 positive
* Asymptomatic or minimally symptomatic metastatic castration-resistant prostate cancer (mCRPC)
* Metastatic castrate-resistant disease defined as one or more of the following criteria that occurred while the patient was on androgen deprivation therapy:

  * Prostate-specific antigen (PSA)-progression defined by Prostate Cancer Working Group 2 (PCWG2) criteria by a minimum of two rising PSA levels with an interval of ≥ 1 week between each determination
  * Progression of nodal metastases defined by Response Evaluation Criteria In Solid Tumors (RECIST) version 1.1 criteria or progression on successive magnetic resonance imaging lymphangiographies (MRLs)
  * Bone disease progression defined by two or more new lesions on bone scan as described in PCWG2 criteria
* Maintenance of castrate circumstances:

  * Ongoing primary androgen deprivation therapy (Gonadotropin-Releasing hormone agonist or antagonist) or bilateral orchiectomy
  * Serum testosterone level ≤ 1.73 nmol/L (50 ng/dL) at screening visit
* PSA value ≥ 2 ng/ml
* Absence of visceral metastases, malignant ascites or pleural effusion
* Clinical absence of brain metastases
* Inclusion within three months after the moment of manifestation of progressive disease as defined above
* Chemotherapy naive
* Life expectancy ≥ 6 months
* World Health Organization/Eastern Cooperative Oncology Group performance status 0-1 (Karnofsky index 100-70)
* White blood cells \>2.0x109/l, neutrophils \>1.5x109/L, lymphocytes \>0.8x109/L, platelets \>100x109/L, hemoglobin \>5,6 mmol/L (9.0 g/dL), serum creatinine \<150 µmol/L, aspartate aminotransferase/alanine aminotransferase \<3 x upper limit of normal (ULN), serum bilirubin \<1.5 x ULN (exception: Gilbert's syndrome is permitted)
* Expected adequacy of follow-up
* Written informed consent

Acceptable concomitant therapy:

* The use of oral or intravenous bisphosphonates
* Radiotherapy for pain relief in patients with bone metastases may be used as a treatment modality, but the need for a radiotherapeutic intervention during the study will be documented as an skeletal-related event (SRE)
* Inhaled corticosteroids and topical creams for small body areas are permitted

Exclusion Criteria:

* Hypercalcemia
* History of any second malignancy in the previous five years, with the exception of adequately treated basal cell carcinoma
* Known allergy to shell fish
* Heart failure (New York Heart Association class III/IV)
* Serious active infections
* Active hepatitis B, C or HIV infection
* Active syphilis infection
* Autoimmune diseases (exception: vitiligo is permitted)
* Organ allografts
* An uncontrolled co-morbidity, e.g. psychiatric or social conditions interfering which participation
* Previous treatment with sipuleucel-T,PROSTVAC, GVAX, chemotherapy, ipilimumab or denosumab (previous treatment with abiraterone acetate, ketoconazole or enzalutamide is permitted)
* Treatment with flutamide, bicalutamide, or nilutamide within four weeks of study enrollment
* Prior radiotherapy within four weeks prior to planned vaccination or presence of treatment-related toxicity
* Continued use of non-steroidal anti-inflammatory drugs
* Concurrent use of systemic corticosteroids \> 10 mg daily prednisone equivalent
* Requirement of opiate use for cancer-related pain (at screening)
* Any serious clinical condition that may interfere with the safe administration of DC vaccinations

Primary outcomes:
measure: The immunogenicity of tumor-peptide loaded natural blood dendritic cells (myeloid DC, plasmacytoid DC and the combination of mDC/pDC) in metastatic castration-resistant prostate cancer (mCRPC) patients
timeFrame: 18 months
measure: The immunogenicity of tumor-peptide loaded natural blood dendritic cells (myeloid DC, plasmacytoid DC and the combination of mDC/pDC) in metastatic castration-resistant prostate cancer (mCRPC) patients
timeFrame: 18 months
measure: The immunogenicity of tumor-peptide loaded natural blood dendritic cells (myeloid DC, plasmacytoid DC and the combination of mDC/pDC) in metastatic castration-resistant prostate cancer (mCRPC) patients
timeFrame: 18 months
Secondary outcomes:
measure: Treatment-related adverse events assessment by Common Terminology Criteria for Adverse Events (CTCAE) version 4.0
timeFrame: 18 months
measure: Quality of life measurement by EORTC-QLQ-C30
timeFrame: 18 months
measure: Quality of life measurement by EORTC-QLQ-PR25
timeFrame: 18 months
measure: Quality of life measurement by BDI (PC)
timeFrame: 18 months
measure: Quality of life measurement by CIS20-R
timeFrame: 18 months
measure: Prostate-specific antigen (PSA)-progression
timeFrame: every 6 weeks, up to 24 months
measure: Progression Free Survival (PFS)
timeFrame: every 6 weeks, up to 24 months
measure: Overall Survival (OS)
timeFrame: 2 years
measure: Time to opiate use
timeFrame: every 3 months, up to 24 months
measure: Time to skeletal-related event (SRE)
timeFrame: every 3 months, up to 24 months
measure: World Health Organization (WHO) performance score decline
timeFrame: every 6 weeks, up to 24 months
measure: Time to chemotherapy initiation after mDC/pDC vaccinations
timeFrame: every 6 weeks, up to 24 months
measure: Radiologic profression-free survival
timeFrame: MRI: every 3 months; 68-Ga-PSMA-PET/CT scan: t=0, 3, 12 en 24 months, up to 24 months.
measure: Feasibility of the natural DC vaccination trial
timeFrame: 18 months
Other outcomes:
Locations:
city: Nijmegen
country: Netherlands
facility: Radboud University Nijmegen Medical Centre
state: Gelderland
Study officials:
affiliation: Radboudumc, dep of Medical Oncology
name: Winald R Gerritsen, MD PhD
role: PRINCIPAL_INVESTIGATOR
affiliation: Radboudumc, dep of Urology
name: Fred Witjes, MD PhD
role: PRINCIPAL_INVESTIGATOR
affiliation: Radboudumc, dep of Tumor Immunology, laboratory study coordinator
name: Jolanda IM de Vries, PhD
role: STUDY_DIRECTOR
References:
citation: Westdorp H, Creemers JHA, van Oort IM, Mehra N, Hins-de Bree SM, Figdor CG, Witjes JA, Schreibelt G, de Vries IJM, Gerritsen WR, Ottevanger PB. High Health-Related Quality of Life During Dendritic Cell Vaccination Therapy in Patients With Castration-Resistant Prostate Cancer. Front Oncol. 2020 Oct 26;10:536700. doi: 10.3389/fonc.2020.536700. eCollection 2020.
citation: Westdorp H, Creemers JHA, van Oort IM, Schreibelt G, Gorris MAJ, Mehra N, Simons M, de Goede AL, van Rossum MM, Croockewit AJ, Figdor CG, Witjes JA, Aarntzen EHJG, Mus RDM, Bruning M, Petry K, Gotthardt M, Barentsz JO, de Vries IJM, Gerritsen WR. Blood-derived dendritic cell vaccinations induce immune responses that correlate with clinical outcome in patients with chemo-naive castration-resistant prostate cancer. J Immunother Cancer. 2019 Nov 14;7(1):302. doi: 10.1186/s40425-019-0787-6.