Clinical trial NCT01790672: Contribution of Wine Components on Hydroxytyrosol Body Concentrations and Biological Effects
Official title: Hydroxytyrosol as an Endogenous and Natural Antioxidant: Modulation by Alcohol Intake. Substudy 2. To Establish the Contribution of Wine Components on Hydroxytyrosol Body Concentrations and Biological Effects
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: PHASE1
Conditions: Contribution of Wine Components in Hydroxytyrosol Formation
Keywords: Antioxidants
Central Nervous System Agents
Wine
Lead sponsor: Parc de Salut Mar
Sponsor class: OTHER
Collaborators: name: Fundacion IMIM
First posted: 2013-02-13
Enrollment: 40
Sex: ALL
Minimum age (years): 18
Maximum age (years): 55
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: CROSSOVER
Primary purpose: BASIC_SCIENCE

Brief summary: The study is aimed at establishing the contribution of wine components on hydroxytyrosol body concentrations and biological effects.


Eligibility criteria: Inclusion Criteria:

* Understanding and accepting the study procedures and signing the informed consent.
* Male and female volunteers aged 18 to 55 years. No fixed sex ratio has been established.
* Clinical history and physical examination demonstrating no organic or psychiatric disorders.
* The ECG and general blood and urine laboratory tests performed before the study should be within normal ranges. Minor or occasional changes from normal ranges are accepted if, in the investigator's opinion, considering the current state of the art, they are not clinically significant, are not life-threatening for the subjects and do not interfere with the product assessment. These changes and their non-relevance will be justified in writing specifically.
* The body mass index (BMI=weigh/height2) will range from 19 to 25 kg/m2, and the weight from 50 to 100 kg. 25-27 could be accepted.
* Women with regular menstrual cycle of 26-32 days.
* Subjects socially drinking and who had ingested wine at least once.

Exclusion Criteria:

* Not meeting the inclusion criteria.
* History or clinical evidence of gastrointestinal, liver, renal or other disorders which may lead to suspecting a disorder in drug absorption, distribution, metabolism or excretion, or that suggest gastrointestinal irritation due to drugs.
* History or clinical evidence of psychiatric disorders, alcoholism, drug abuse, or regular use of psychoactive drugs.
* Blood donation in the previous 8 weeks or participation in other clinical trials with drugs in the previous 12 weeks.
* Having suffered any organic disease or major surgery in the three months prior to the study start.
* Subjects with intolerance or serious adverse reactions to ethanol.
* Regular use of any drug in the month prior to the study sessions, except for vitamins or diet supplements, that, in the opinion of the principal investigator or co-investigators designated by him, do not involve a risk for the subject and do not interfere with the study objectives. In this case they should be discontinued from one week before the experimental sessions to the end of them. The treatment with single or limited doses of symptomatic medicinal products in the week prior to the study sessions will not be a reason for exclusion if it is calculated that it has been cleared completely the day of the experimental session.
* Taking more than 20 g of alcohol a day in women and more than 30 g in men.
* Taking more than 5 coffees, teas, cola drinks or other stimulating drinks or with xanthines daily in the 3 months prior to the study start.
* Subjects unable to understand the nature, consequences of the study and the procedures requested to be followed.
* Smokers or ex-smokers of less than one year.
* Subjects with positive serology to Hepatitis B, C or HIV.
* Pregnant or nursing women or positive pregnancy test in urine. Women not undertaking to follow reliable contraceptive measures during the study (such as abstinence, hormonal contraceptives, intrauterine devices, double-barrier methods or vasectomised partner).
* Women with amenorrhea or premenstrual syndrome of moderate or severe intensity.
* Nonsteroidal anti-inflammatory drugs, antioxidants or vitamins consumption in the last 2 weeks.
* Total cholesterol \>6.00 mmol/l (240 mg/dl) or triglycerides \>2.26 mmol/l (200 mg/dl).
* Vegetarians or subjects following aberrant diets.
* Physical exercise of \>2h/day or \>3000 kcal /week.

To be eligible, the subjects must agree to follow a poor polyphenols diet in the 72 hours prior to the start of each session and until 24 hours after.

Primary outcomes:
measure: Hydroxytyrosol biological fluids concentrations
timeFrame: 0-24h after administration
Secondary outcomes:
measure: Ethanol concentration
timeFrame: 0-6h after administration
measure: Subjective drunkenness
timeFrame: 0-6h after administration
measure: Vital signs
timeFrame: 0-24h after administration
measure: Ethanol metabolites concentrations
timeFrame: 0-24h after administration
measure: Dopamine metabolites concentrations
timeFrame: 0-24h after administration
measure: Oxidation biomarkers of proteins and lipids
timeFrame: 0-24h after administration
measure: Psychomotor performance (DSST)
timeFrame: 0-6h after administration
measure: Gene expression of genes related with receptors and regulators/oxidative stress and antioxidant defence
timeFrame: Baseline
measure: Platelets monoamine oxidase-B activity
timeFrame: Baseline
measure: Breath ethanol
timeFrame: 30 min after administration
Other outcomes:
Locations:
city: Barcelona
country: Spain
facility: Parc de Salut Mar
Study officials:
affiliation: Parc de Salut Mar
name: Magí Farré, MD, PhD
role: PRINCIPAL_INVESTIGATOR
References: