Clinical trial NCT05476731: Phenotypic and Genotypic Characterization of Malassezia Species Isolated From Malassezia Associated Skin Diseases
Official title: Phenotypic and Genotypic Characterization of Malassezia Species Isolated From Malassezia Associated Skin Diseases
Study type: OBSERVATIONAL
Overall status: UNKNOWN
Conditions: Malassezia Infection
Lead sponsor: Sohag University
Sponsor class: OTHER
First posted: 2022-07-27
Enrollment: 100
Sex: ALL
Standard ages: CHILD
ADULT
OLDER_ADULT
Accepts healthy volunteers: no
Observational model: CASE_CONTROL
Time perspective: CROSS_SECTIONAL
Sampling method: PROBABILITY_SAMPLE
Study population: Characteristic lesions of hypopigmented or hyperpigmented scaly macules and patches which may be associated with itching in patients with Pityriasis versicolor and scales of Seborrheic dermatitis and scales from patients with Atopic dermatitis.

Brief summary: Genus Malassezia includes 14 species, namely M. furfur, M. sympodialis, M. globosa, M. restricta, M. slooffiae, M. obtusa, M. dermatis, M. japonica and M. yamatoensis associated with normal human flora but can also cause skin lesions, and M. pachydermatis, M. nana, M. equina, M. caprae and M. cuniculiare associated with animals. Few studies found that M. pachydermatis may be transmitted to humans from pets. M. pachydermmatis is the only lipid independent species while others are lipid dependent .

As Malassezia species have similar morphological and biochemical features, the currently used phenotypic techniques for diagnosis of Pityriasis versicolor usually do not allow rapid and exact characterization. In addition; they are time-consuming, multi-step processes requiring several experimental methods. Therefore, several molecular typing methods have been used successfully, resulting in identification and classification of new Malassezia species

Aim of the work:

1. Phenotypic and Genotypic Characterization of Malassezia Species.
2. Identify antifungal resistance pattern among isolated fungus.


Eligibility criteria: Inclusion Criteria:

Characteristic lesions of hypopigmented or hyperpigmented scaly macules and patches which may be associated with itching in patients with Pityriasis versicolor and scales of Seborrheic dermatitis and scales from patients with Atopic dermatitis.

Exclusion Criteria:

Patients with skin lesions of extensive desquamation or inflammation that do not match Pityriasis versicolor , Seborrheic dermatitis or Atopic dermatitis. Patients who had received topical antifungal therapy within last three months or oral antifungal therapy within the last six months

Primary outcomes:
measure: frequency of malassezia in patients
timeFrame: 6 months
Secondary outcomes:
Other outcomes:
Locations:
city: Sohag
country: Egypt
facility: Sohag University Hospital
Study officials:
References:
citation: Angiolella L, Carradori S, Maccallini C, Giusiano G, Supuran CT. Targeting Malassezia species for Novel Synthetic and Natural Antidandruff Agents. Curr Med Chem. 2017;24(22):2392-2412. doi: 10.2174/0929867324666170404110631.
citation: Gaitanis G, Magiatis P, Hantschke M, Bassukas ID, Velegraki A. The Malassezia genus in skin and systemic diseases. Clin Microbiol Rev. 2012 Jan;25(1):106-41. doi: 10.1128/CMR.00021-11.
citation: Lee YW, Byun HJ, Kim BJ, Kim DH, Lim YY, Lee JW, Kim MN, Kim D, Chun YJ, Mun SK, Kim CW, Kim SE, Hwang JS. Distribution of malassezia species on the scalp in korean seborrheic dermatitis patients. Ann Dermatol. 2011 May;23(2):156-61. doi: 10.5021/ad.2011.23.2.156. Epub 2011 May 27.
citation: Lyakhovitsky A, Shemer A, Amichai B. Molecular analysis of Malassezia species isolated from Israeli patients with pityriasis versicolor. Int J Dermatol. 2013 Feb;52(2):231-3. doi: 10.1111/j.1365-4632.2012.05595.x.