Clinical trial NCT05297955: Circulating Tumor Cell Detection in Hepatocellular Carcinoma
Official title: A Retrospective Study of Data Related to Circulating Tumor Cell Detection in Hepatocellular Carcinoma
Study type: OBSERVATIONAL
Overall status: COMPLETED
Conditions: Circulating Tumor Cell
Lead sponsor: Tongji Hospital
Sponsor class: OTHER
First posted: 2022-03-28
Enrollment: 458
Sex: ALL
Minimum age (years): 18
Maximum age (years): 80
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: no
Observational model: COHORT
Time perspective: RETROSPECTIVE
Sampling method: NON_PROBABILITY_SAMPLE
Study population: Perioperative patients with hepatocellular carcinoma who underwent CTC detection of peripheral blood

Brief summary: The investigators examined circulating tumor cells (CTC) in the perioperative peripheral blood of hundreds of HCC patients undergoing liver cancer surgery using CellSearch technology between 2013 and 2016. Although the investigators have done a preliminary study of the above data and published some results, the previous study was only a basic analysis. Now the investigators plan to carry out further in-depth analysis of these data, including hospitalization data, follow-up results, surgical tumors and blood specimens, and make full use of biostatistics, molecular biology, pathology and other related techniques to elucidate the association between the levels of CTC or CTC clusters and patients' disease during the perioperative period, and to explore the molecular basis of CTC production in hepatocellular carcinoma.

Detailed description: From 2013 to 2016, CellSearch technology was used to detect the number of circulating tumor cells (CTC) in peripheral blood of hundreds of HCC patients undergoing liver cancer surgery during perioperative period. The analysis results confirmed that the level of CTC before and after surgery were significantly correlated with overall survival (OS) and disease-free survival (DFS) of patients. CTC level changed before and after surgery, but there was no statistical difference. Preoperative CTC was more correlated with patients' disease-related clinical parameters, while postoperative CTC was an independent prognostic indicator of patients after surgery. In addition, the investigators' study found that CTC clusters level can be a new marker of tumor progression in HCC patients.

Based on the above data, the investigators will perform retrospective analysis to compare the relationship between CTC level and clinical indicators of HCC patients. Participants were divided into groups according to pathological type, differentiation degree, clinical stage, invasion degree, recurrence and metastasis of tumors. Combined with immunohistochemistry and quantitative PCR results, the correlation of CTC in tumor genesis, pathological model, degree of differentiation, tumor stage, invasion, metastasis and expression of other tumor markers was studied.

Eligibility criteria: Inclusion Criteria:

* The pathological diagnosis was primary liver cancer.
* Undergoing radical surgical treatment
* No preoperative antitumor therapy was received
* Between 18 and 80 years old

Exclusion Criteria:

* With distal metastasis
* With other tumors
* Perioperative death
* Recurrence within one month after surgery
* Lost contact before the first follow-up

Primary outcomes:
measure: Data analysis of relationship between CTC and pathological indicators of hepatocellular carcinoma
timeFrame: 2022.03.15
Secondary outcomes:
Other outcomes:
Locations:
city: Wuhan
country: China
facility: Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology
state: Hubei
Study officials:
affiliation: Tongji Hospital
name: Xiaoping Chen
role: STUDY_CHAIR
References: