Clinical trial NCT05274373: Clinical, Virological, Serological and Immunological Characteristics During and Following COVID-19 Hospitalization
Official title: Clinical, Virological, Serological and Immunological Characteristics During and Following COVID-19 Hospitalization: a Prospective Cohort Study
Study type: OBSERVATIONAL
Overall status: UNKNOWN
Conditions: COVID-19 Pneumonia
COVID-19
Keywords: Neutralizing Antibodies
Viral Load
Antibody Decay
Viral Culturing
Infectiousness
Lead sponsor: Nordsjaellands Hospital
Sponsor class: OTHER
Collaborators: name: Statens Serum Institut
name: Rigshospitalet, Denmark
First posted: 2022-03-10
Enrollment: 47
Sex: ALL
Minimum age (years): 18
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: no
Observational model: COHORT
Time perspective: PROSPECTIVE
Sampling method: NON_PROBABILITY_SAMPLE
Study population: Adult unvaccinated hospitalized patients

Brief summary: Assessment of the association between the severity of COVID-19 and SARS-CoV-2 NAb titers levels for up to six months following primary infection using a live virus NAb assay. Description of SARS-CoV-2 viral shedding and infectiousness during the first 30 days after infection in a group of unvaccinated hospitalized patients.

Detailed description: The studys primary aim is to assess clinical factors, such as disease severity, associated with neutralizing antibody (NAb) production. Furthermore, the study aims to assess the length of SARS-CoV-2 infectiousness and clinical factors associated with viral load.

Patients 18 years or older hospitalized at Copenhagen University Hospital at North Zealand, Copenhagen, Denmark, May 24th,2020 - May 5th, 2021, were routinely screened for COVID-19 by diagnostic oropharyngeal or tracheal RT-PCR samples taken during admission. Patients with a positive SARS-CoV-2 PCR within 48 hours from hospital admission were offered inclusion, if COVID-19 pneumonia was confirmed.

The following were retrieved from patients' electronic records: comorbidities (Charlson Comorbidity Index),vital signs (Early Warning Score), immunocompromised status, time from symptom onset to admission, oxygen treatment, pharmacological treatment, admission length, death and bacterial co-infection.

Paired oropharyngeal swabs and serum samples were collected at inclusion (day 0), days 3, 7, 10, 14, 17, 24, and 30. Serum samples were, if possible, also collected after three and six months. Follow-up time was six months.

RT-qPCR analysis targeted the SARS-CoV-2 RNA-dependent-RNA-polymerase (RdRp)-helicase gene region and two samples with known viral load were included in each PCR-run for quantification of patient samples. Virus was cultured in African green monkey cells (VERO-E6) with incubation for 3 - 4 days and daily microscopic inspection for cytopathogenic effect (CPE). A total of three passages were made before the the virus was interpreted as non-replicant. Cells with CPE were confirmed by RT-qPCR.

The presence of specific antibodies (Ab) against SARS-CoV-2 in serum was assessed using Wantai total-Ab ELISA according to the manufacturer's instructions (Wantai, Beijing, China).

For the in-house live virus NAb analysis, a 50% cut-off value was calculated from quadruplicate virus and cell control wells included on each plate using the following equation: (average optical density (OD) of virus control wells + average OD of cell control wells)/2. The 50% neutralization titer was calculated as the interpolation of the cutoff value with a four-parameter logistic regression curve fitted for each serum serial dilution. To minimize inter-assay variation, the titers were normalized according to a positive control included on each assay plate.

A linear mxied-effects model was used to assess the association between repeated NAb measurements and clinical variables such as age, gender and disease severity. A linear mixed-effects model was also used to assess the association between repeated viral load measurements and clinical variables.

Eligibility criteria: Inclusion Criteria:

* positive SARS-CoV-2 specimen from upper or lower respiratory tracts (virological criteria)
* consolidations on the chest X-ray described by a radiologist, treating physician or a physician from the study group (radiological criteria)
* the presence of one or more of the following: fever (temperature ≥38.0°C), new-onset cough, pleuritic chest pain, dyspnea or altered breath sounds on auscultation (clinical criteria)

Exclusion Criteria:

* cognitive impairment prohibiting giving informed consent to participation
* by December 14th, 2020, and onwards patients if the time since symptom onset was \> seven days at the time of inclusion

Primary outcomes:
measure: Neutralizing Antibody Titer - Day 0
timeFrame: Day 0
measure: Change from baseline in Neutralizing Antibody Titer - Day 30
timeFrame: Day 30
measure: Change from baseline in Neutralizing Antibody Titer - Day 90
timeFrame: Day 90
measure: Change from baseline in Neutralizing Antibody Titer- Day 180
timeFrame: Day 180
measure: Viral culturing - Day 0
timeFrame: Day 0
measure: Viral culturing - Day 3
timeFrame: Day 3
measure: Viral culturing - Day 7
timeFrame: Day 7
measure: Viral culturing - Day 10
timeFrame: Day 10
measure: Viral culturing - Day 14
timeFrame: Day 14
measure: Viral culturing - Day 17
timeFrame: Day 17
measure: Viral culturing - Day 24
timeFrame: Day 24
measure: Viral culturing - Day 30
timeFrame: Day 30
Secondary outcomes:
measure: Baseline Viral Load - Day 0
timeFrame: Day 0
measure: Change from Baseline Viral Load - Day 3
timeFrame: Day 3
measure: Change from Baseline Viral Load - Day 7
timeFrame: Day 7
measure: Change from Baseline Viral Load - Day 10
timeFrame: Day 10
measure: Change from Baseline Viral Load - Day 14
timeFrame: Day 14
measure: Change from Baseline Viral Load - Day 17
timeFrame: Day 17
measure: Change from Baseline Viral Load - Day 24
timeFrame: Day 24
measure: Change from Baseline Viral Load - Day 30
timeFrame: Day 30
Other outcomes:
Locations:
city: Copenhagen
country: Denmark
facility: Copenhagen University Hospital at North Zealand
Study officials:
affiliation: Nordsjaellands Hospital
name: Zitta Barrella Harboe, MD, PhD
role: PRINCIPAL_INVESTIGATOR
References: