Clinical trial NCT06083610: MicroRNA-1 (miRNA-1) and MicroRNA-133a (miRNA-133a) Levels After Acute Exercise in Ultimate Frisbee Athletes
Official title: Investigation of MicroRNA-1 (miRNA-1) and MicroRNA-133a (miRNA-133a) Levels After Acute Neuromuscular Exercise in Ultimate Frisbee Athletes
Study type: OBSERVATIONAL
Overall status: RECRUITING
Conditions: To Determine the Levels of miRNA1 and miRNA133a Before and After Exercise
Keywords: microRNA
acute exercise
skeletal muscle
exercise training
Lead sponsor: Yeditepe University
Sponsor class: OTHER
First posted: 2023-10-16
Enrollment: 34
Sex: ALL
Minimum age (years): 18
Maximum age (years): 25
Standard ages: ADULT
Accepts healthy volunteers: yes
Observational model: COHORT
Time perspective: CROSS_SECTIONAL
Sampling method: NON_PROBABILITY_SAMPLE
Study population: Ultimate Frisbee team athletes of a university

Brief summary: "The goal of this clinical trial is to investigate the levels of microRNA-1 (miRNA-1) and microRNA-133a (miRNA-133a) after acute neuromuscular exercise (NME) in human saliva.

The main questions it aims to answer are:

* How do miRNA-1 and miRNA-133a levels change in response to acute neuromuscular exercise in human saliva?
* Will the chosen training method have an acute effect in ultimate frisbee athletes?

Participants will:

* Undergo acute neuromuscular exercise (NME).
* Provide saliva samples for miRNA analysis.

Researchers will compare:

MiRNA-1 and miRNA-133a levels before and after acute neuromuscular exercise (NME) to determine if exercise induces any significant changes in these microRNA levels."

Detailed description: The genetic material in humans and other living things is DNA, and the information in DNA is transcribed into RNA, and RNA translates this information into protein through translation. More than 98% of RNA cannot be translated into protein this untranslated part is called non-coding RNA. Of the non-coding RNAs, micro-RNAs (miRNAs) are short RNAs of approximately 21-23 nucleotides in length that do not code for proteins and that control gene expression after transcription. miRNAs can partially bind to target messenger RNAs (mRNAs), suppressing protein production or silencing gene expression by splicing target mRNAs. miRNAs enable cell differentiation and development by using the translational repression mechanism. In addition to these roles, miRNAs play a role in many points such as cellular metabolism, repair mechanisms, signaling, cell division, and apoptosis pathways. Studies also identify miRNAs as essential intracellular mediators of processes involved in exercise adaptation, including angiogenesis, inflammation, mitochondrial metabolism, cardiac and skeletal muscle contraction force generation, and tissue hypertrophy. There is evidence that the miRNA may play an important role in regulating the plasticity of the skeletal muscles, such as the hypertrophy of the skeletal muscles, or in regulating the functional properties of the muscle. More than 2000 miRNAs have been identified in humans, and some of them are found and function in the human body in a tissue-specific manner. For example, miRNAs, mostly found in muscle tissue and therefore called myomiRs, are involved in proliferation, muscle cell differentiation, metabolism, and hypertrophy in skeletal and cardiac muscles. The myomiR class includes miR-1, miR-133a, miR-133b, miR-206, miR-208a, miR-208b, miR-486, and miR-499. In the literature, studies examine miRNA levels before and after using various exercise modalities. It has been stated that different miRNAs provide responses such as ventricular compliance, hypertrophy, angiogenesis, and mitochondrial biogenesis after acute or chronic exercise. It has been shown in many studies that these responses, decreased or increased miRNA levels after exercise, are related to the target genes they affect, such as IGF-1, PGC-1α, MAPK, TGF-β, COL1A1, and FOXJ3.

The aim of the study is to investigate the levels of miRNA-1 and miRNA-133a levels after acute neuromuscular exercise (NME) in human saliva.

Eligibility criteria: Inclusion Criteria:

* Not suffering from an acute illness
* Not having had an orthopedic injury in the last 12 weeks may prevent them from exercising
* Volunteering to participate in the study

Exclusion Criteria:

* Presence of an acute disease
* Having an orthopedic injury that may prevent exercise in the last 12 weeks
* Regular use of medication and/or dietary supplements
* Presence of a chronic \& systemic disease
* Not volunteering to participate in the study

Primary outcomes:
measure: Determination of miRNA-1 and miRNA-133a levels before and after acute neuromuscular exercise
timeFrame: Approximately 1.5 hours for each participant
Secondary outcomes:
Other outcomes:
Locations:
city: Istanbul
country: Turkey
facility: Yeditepe University
Study officials:
affiliation: Yeditepe University
name: Turgay İsbir, Prof. Dr.
role: PRINCIPAL_INVESTIGATOR
References:
citation: Safdar A, Abadi A, Akhtar M, Hettinga BP, Tarnopolsky MA. miRNA in the regulation of skeletal muscle adaptation to acute endurance exercise in C57Bl/6J male mice. PLoS One. 2009;4(5):e5610. doi: 10.1371/journal.pone.0005610. Epub 2009 May 19.
citation: McCarthy JJ, Esser KA. MicroRNA-1 and microRNA-133a expression are decreased during skeletal muscle hypertrophy. J Appl Physiol (1985). 2007 Jan;102(1):306-13. doi: 10.1152/japplphysiol.00932.2006. Epub 2006 Sep 28.
citation: Ultimo S, Zauli G, Martelli AM, Vitale M, McCubrey JA, Capitani S, Neri LM. Influence of physical exercise on microRNAs in skeletal muscle regeneration, aging and diseases. Oncotarget. 2018 Mar 30;9(24):17220-17237. doi: 10.18632/oncotarget.24991. eCollection 2018 Mar 30.