Clinical trial NCT05131555: Influence of Endurance Exercise and Histamine Receptors on the Gene Expression in Skeletal Muscle
Official title: Influence of Endurance Training and Histamine Receptor Antagonists on the Transcriptome Response in Human Muscle
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Exercise
Histamine
Lead sponsor: University Ghent
Sponsor class: OTHER
Collaborators: name: Research Foundation Flanders
First posted: 2021-11-23
Enrollment: 14
Sex: ALL
Minimum age (years): 18
Maximum age (years): 45
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: CROSSOVER
Intervention model description: Randomized double-blinded placebo-controlled cross-over interventional study
Primary purpose: BASIC_SCIENCE

Brief summary: Blocking histamine H1/H2 receptors blunts chronic endurance training adaptations. The current study addresses a twofold research question: "What is the influence of endurance training (1) and histamine H1 and H2 signaling (2) on the gene expression in human skeletal muscle." Results from this study will yield more insights into the molecular mechanisms of adaptations to exercise training.


Eligibility criteria: Inclusion Criteria:

* Male and female
* 18-45 years
* not to medium physically active

Exclusion Criteria:

* Smoking
* Chronic disease
* Supplement or medication intake
* Seasonal allergies

Primary outcomes:
measure: Change in muscle transcriptome (direct response)
timeFrame: On each test-day: 0 minutes after the exercise training versus at rest.
measure: Change in muscle transcriptome (delayed response)
timeFrame: On each test-day: 180 minutes after the exercise training versus at rest.
measure: Muscle glycogen depletion
timeFrame: On each test-day: 0 minutes after the exercise training versus at rest.
measure: Muscle glycogen resynthesis
timeFrame: On each test-day: 180 minutes after the exercise training versus at rest.
measure: Plasma volume change
timeFrame: Change from rest to different time-points after exercise (0, 30, 60, 120 and 180 minutes after the exercise training).
Secondary outcomes:
measure: Heart rate during the exercise training
timeFrame: Continuously during training on each test-day.
measure: Blood lactate
timeFrame: On each test-day at 10 time-points: at rest, during exercise and 0, 30, 60, 120 and 180 minutes after the exercise training.
measure: Blood glucose
timeFrame: On each test-day at 10 time-points: at rest, during exercise and 0, 30, 60, 120 and 180 minutes after the exercise training.
measure: Blood histamine
timeFrame: On each test-day at 6 time-points: at rest and 0, 30, 60, 120 and 180 minutes after the exercise training.
measure: Blood insulin
timeFrame: On each test-day at 6 time-points: at rest and 0, 30, 60, 120 and 180 minutes after the exercise training.
measure: Muscle signaling pathways relevant for glucose metabolism and cardiovascular health
timeFrame: On each test-day: at rest, 0 minutes and 180 minutes after the exercise training.
Other outcomes:
Locations:
city: Ghent
country: Belgium
facility: Department of movement and sports sciences, Ghent University, Belgium
state: Oost-Vlaanderen
Study officials:
affiliation: University Ghent
name: Wim Derave, Professor
role: PRINCIPAL_INVESTIGATOR
References: