Clinical trial NCT06905964: Eccentric Cycling Exercise With Assistive Devices on Mitochondrial Metabolism in T Lymphocyte
Official title: Effects of Eccentric Cycling Exercise Training With Assistive Devices on Phenotypes and Mitochondrial Metabolism in T Lymphocytes in Sedentary Men
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: There Are no Conditions Under Study. Healthy Volunteers
Keywords: Eccentric cycling exercise
Neuromuscular electrical stimulation
Compressive garment
Lymphocyte
Mitochondria
Lead sponsor: Chang Gung Memorial Hospital
Sponsor class: OTHER
First posted: 2025-04-02
Enrollment: 30
Sex: MALE
Minimum age (years): 20
Maximum age (years): 30
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: PARALLEL
Primary purpose: BASIC_SCIENCE

Brief summary: Eccentric cycling exercise can be a promising modality fwith lower respiratory demands and higher power output. Assistive devices like neuromuscular electrical stimulation (NMES) and compressive garment (CG) accelerates muscle pumping and relieve the eccentric exercise-induced muscle soreness.

Phenotypes and mitochondrial metabolism are crucial to regulate acquired immnunity of T lymphocytes. The study aimed to investigate the effects of eccentric cycling exercise training combined with NMES and CG on mitochondrial phenotypes and bioenergetics in T lymphocytes in sedentary males. The hypothesis were an acute/intense exercise decreased the mitochondrial respiratory capacities of OXPHOS, ETS and bioenergetics, while eccentric cycling training could elevate the diminished mitochondrial functions.

Methods: Twelve sedentary healthy males were randomly assigned to either eccentric cycling with assistive devices (ECA, n=10) or eccentric cycling only (ECC, n=10). All participants performed ECA or ECC at an intensity of 60% to 120% maximal workload progressively on a costumed bicycle ergometer for 40 min/day, 5 days/week for 5 weeks. ECA performed NMES for 20min/session after cycling, while ECC was seated for resting 20 min. Systemic aerobic fitness, phenotypes of T lymphocytes, mitochondrial OXPHOS, ETS respiratory capacities, and bioenergetics in T lymphocytes were analyzed.


Eligibility criteria: Inclusion Criteria:

* Sedentary lifestyle (exercise\<20min/session; \<1session/week for a year or longer).
* Nonsmokers, nonusers of medications/vitamins.
* No any cardiopulmonary, neuromuscular, and hematological risks.

Exclusion Criteria:

* Have regular exercise habits (i.e., exercise frequency once per week, duration \>20 min).
* Have retabolic or musculoskeletal disease.

Primary outcomes:
measure: Mitochondrial bioenergetics of oxygen consumption rate in T lymphocyte
timeFrame: At baseline and 2 days after completing the 5-week intervention
measure: Systemic aerobic fitness (VO2)
timeFrame: At baseline and 2 days after completing the 5-week intervention
Secondary outcomes:
Other outcomes:
Locations:
city: Guishan
country: Taiwan
facility: Chang Gung University
state: Taoyuan
Study officials:
References:
citation: Huang YC, Cheng ML, Tang HY, Huang CY, Chen KM, Wang JS. Eccentric Cycling Training Improves Erythrocyte Antioxidant and Oxygen Releasing Capacity Associated with Enhanced Anaerobic Glycolysis and Intracellular Acidosis. Antioxidants (Basel). 2021 Feb 13;10(2):285. doi: 10.3390/antiox10020285.
citation: Peng SC, Lin YT, Hsu CC, Fu TC, Wang JS. Effects of concentric and eccentric exercise regimens on bioenergetic efficiency of lymphocytes in sedentary males. Eur J Sport Sci. 2024 Dec;24(12):1830-1843. doi: 10.1002/ejsc.12221. Epub 2024 Nov 9.
citation: Huang YC, Hsu CC, Fu TC, Wang JS. Interval aerobic/resistance exercise training depresses adrenergic-induced apoptosis of lymphocytes in sedentary males. Eur J Appl Physiol. 2024 Mar;124(3):837-848. doi: 10.1007/s00421-023-05311-3. Epub 2023 Sep 15.