Clinical trial NCT06082050: Efficacy and Safety of Intravenous YOLT-201 for Transthyretin Amyloidosis Cardiomyopathy
Official title: Efficacy and Safety of Intravenous YOLT-201 for Transthyretin Amyloidosis Cardiomyopathy
Study type: INTERVENTIONAL
Overall status: RECRUITING
Phase: EARLY_PHASE1
Conditions: Transthyroxin Amyloidosis Cardiomyopathy
Lead sponsor: Zhejiang University
Sponsor class: OTHER
Collaborators: name: YolTech Therapeutics Co., Ltd
First posted: 2023-10-13
Enrollment: 7
Sex: ALL
Minimum age (years): 18
Maximum age (years): 80
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: no
Allocation: NA
Intervention model: SINGLE_GROUP
Primary purpose: TREATMENT

Brief summary: This study is a single-arm, open-label, single-dose escalation trial aimed at evaluating the safety and tolerability of YOLT-201 treatment in patients with Transthyretin Amyloidosis Cardiomyopathy （ATTR-CM）, as well as assessing the preliminary efficacy of subjects treated with YOLT-201.


Eligibility criteria: Inclusion Criteria:

1. Male or female subjects aged ≥18 and ≤80 years old at the time of signing informed consent.
2. Diagnosis of transthyretin cardiac amyloidosis (ATTR-CM) based on the 2021 "Chinese Expert Consensus on the Diagnosis and Treatment of Transthyretin Cardiac Amyloidosis," the 2021 European Society of Cardiology (ESC) guidelines, and the 2023 American College of Cardiology (ACC) guidelines, including hereditary ATTR cardiac amyloidosis (ATTRv-CM) and wild-type ATTR cardiac amyloidosis (ATTRwt-CM); and relevant indicators meet the following criteria:

   2.1 New York Heart Association (NYHA) functional classification of I to III; 2.2 6-minute walk test (6-MWT) ≥ 150 meters at screening; 2.3 Echocardiogram evidence of cardiac involvement: left ventricular wall thickness (interventricular septum and/or posterior wall thickness ≥12 mm); 2.4 Brain natriuretic peptide N-terminal prohormone (NT-proBNP) \< 2000 pg/mL at screening; 2.5 Receiving stable and standardized cardiovascular medication treatment.
3. Body weight must not be less than 40 kg at screening.
4. Subjects must meet the following laboratory criteria at screening:

   4.1 Aspartate aminotransferase (AST), alanine aminotransferase (ALT), total bilirubin (except for Gilbert's syndrome), International Normalized Ratio (INR) \< upper limit of normal (ULN); 4.2 Subjects with a history of Gilbert's syndrome have a total bilirubin not exceeding twice the upper limit of normal (ULN) during the screening period; 4.3 Estimated glomerular filtration rate (eGFR) \> 45 mL/min/1.73m\^2 at screening; 4.4 Platelet count ≥ 100 × 10\^9/L; 4.5 Coagulation function at screening: fibrinogen, activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT) are all within the normal range; 4.6 Low-density lipoprotein (LDL) cholesterol \< 200 mg/dL; 4.7 Vitamin A level ≥ lower limit of normal (LLN); 4.8 Vitamin B12 level ≥ lower limit of normal (LLN).
5. Acceptance of ATTR-CM medication treatment:

   5.1 Unable to receive existing ATTR-CM-related medication due to regional medical resources, health policies, personal financial capacity, or drug intolerance; 5.2 Despite receiving at least 6 months of ATTR-CM medication treatment, the subject's ATTR-CM condition still progresses (meeting at least one of the following): Increased hospitalizations related to heart failure; Worsening of NYHA classification; 6-MWT decrease of at least 30 meters; NT-proBNP increase by 30%; Troponin increase by 30%; Echocardiogram indicates: increase in left ventricular wall thickness by 2 mm, or decrease in left ventricular ejection fraction by ≥5%, or decrease in global longitudinal strain by ≥1%, or decrease in stroke volume by ≥5%, or worsening diastolic function; New conduction block on electrocardiogram.
6. No intake of alcohol from the start of the screening period to 28 days after receiving the trial treatment medication.
7. Female subjects must be postmenopausal for at least one year, or have undergone hysterectomy.
8. Male subjects and their partners must use highly effective contraceptive measures recognized by the doctor throughout the trial process and for at least 5 months after the trial ends.
9. Male subjects must not donate sperm within 84 days after receiving study medication.
10. Voluntary signing of informed consent.

Exclusion Criteria:

1. Amyloidosis that is not related to TTR protein degeneration, such as immunoglobulin light chain (AL) amyloidosis.
2. History of multiple myeloma.
3. Indeterminate monoclonal gammopathy (MGUS) and/or immunoglobulin free light chain (FLC) ratio alterations, unless fat, bone marrow, or cardiac biopsy confirms the absence of light chains but presence of TTR protein through mass spectrometry or immunoelectron microscopy. For chronic kidney disease (CKD) subjects with no monoclonal protein in blood and urine, the acceptable FLC ratio is 0.26 to 2.25. If the risks of biopsy outweigh the benefits, different results may be discussed with local hematologists, investigators, and medical monitors.
4. Allergy to any lipid nanoparticle (LNP) components, or history of exposure to LNP components or laboratory abnormalities or adverse reaction events related to their treatment: 4.1 Normal baseline, ALT or AST \> 3 × ULN or appearance of baseline triple value after receiving LNP product. 4.2 Normal baseline, INR, APTT, or D-dimer \> 1.5 × ULN; if baseline is higher than normal after receiving LNP product, it is 1.5 times the baseline value. 4.3 Any adverse reactions related to LNP product treatment are defined as grade 3 or higher (CTCAE). Injection site reactions (IRR) requiring treatment or discontinuation of infusion; slowing down the infusion rate to alleviate infusion-related reactions. The investigator believes that any adverse reaction events related to LNP treatment should be excluded.
5. Use of any of the following ATTR treatments within a certain period:

   5.1 Patisiran (LNP small interfering RNA siRNA) treatment product (within 90 days after administration).

   5.2 Previous treatment with Inotersen (antisense oligonucleotide ASO) (within 160 days after administration).

   5.3 Previous use of Vutrisiran (investigational siRNA therapeutic GalNAc conjugate).

   5.4 Tafamidis (TTR stabilizer): less than 10 days since last drug administration before study drug administration.

   5.5 Diflunisal (TTR stabilizer): less than 3 days since last drug administration before study drug administration.

   5.6 Doxycycline and/or tauroursodeoxycholic acid (TTR chaperone): less than 14 days since last drug administration before study drug administration.

   5.7 Any other drugs used for the treatment of ATTR-CM: less than 30 days or 5 half-lives (whichever is longer) since last drug administration before study drug administration.
6. Other organic heart diseases, such as ischemic heart disease, uncontrolled hypertension, valvular heart disease, etc., leading to cardiomyopathy.
7. Current or previous NYHA class IV symptoms or worsening heart failure symptoms within 90 days before or during screening.
8. Hospitalization or invasive surgery due to cardiovascular and cerebrovascular diseases within 90 days before or during screening. For example, acute coronary syndrome, unstable angina, stroke, TIA, coronary artery reconstruction, cardiac device implantation, valvular repair, or major surgery, etc.
9. Expected to undergo invasive cardiovascular surgery (such as coronary artery stent, pacemaker implantation, etc.) within 28 days after medication.
10. Subjects who cannot or are unwilling to supplement vitamin A.
11. Subjects who cannot or are unwilling to accept the required medication treatment plan before treatment.
12. Antiplatelet (such as aspirin, clopidogrel) or antithrombotic treatment (such as warfarin, dabigatran, apixaban) within 14 days before study drug administration.
13. History of bleeding diathesis or coagulation disorders (such as liver cirrhosis, malignant hematological diseases, antiphospholipid antibody syndrome).
14. The investigator believes that the expected survival is less than 2 years.
15. Ophthalmological examination results consistent with vitamin A deficiency.
16. Known or suspected systemic viral, parasitic, or fungal infections, or expected to receive antibiotic treatment for bacterial infections within 14 days after screening.
17. History of hepatitis B or C infection or positive for hepatitis B surface antigen (HBsAg) or hepatitis C virus antibody (HCV Ab) at screening.
18. History of human immunodeficiency virus (HIV) positivity.
19. Clinically significant thyroid function abnormalities judged by the investigator.
20. History of liver, heart, or other solid organ transplantation or bone marrow transplantation within 1 year at screening, or expected transplantation. Note: No history or plan for corneal transplantation.
21. History of active malignant tumors within 5 years before screening, except for basal cell carcinoma of the skin, cured squamous cell carcinoma of the skin, cured cervical cancer, or low-grade prostate cancer under observation.
22. History of alcohol abuse or drug abuse within 3 years before screening.
23. Female subjects with childbearing potential or who are breastfeeding.
24. Other physical, social, or psychological diseases, including the use of illicit drugs or alcohol, which the investigator believes may adversely affect the subject's safety, impair the assessment of study results, or prevent the subject from complying with any conditions of this study, laboratory abnormalities, or other reasons.
25. Unwilling to comply with study procedures, including follow-ups as specified in the protocol, or unwilling to cooperate fully with the investigator.

Primary outcomes:
measure: Adverse event Adverse event Adverse events
timeFrame: hrough study completion, an average of 1 year
Secondary outcomes:
measure: Cmax
timeFrame: hrough study completion, an average of 1 year
measure: Tmax
timeFrame: hrough study completion, an average of 1 year
measure: AUCinf
timeFrame: through study completion, an average of 1 year
measure: AUClast
timeFrame: through study completion, an average of 1 year
measure: t1/2
timeFrame: through study completion, an average of 1 year
measure: CL/F
timeFrame: through study completion, an average of 1 year
measure: change of TTR
timeFrame: through study completion, an average of 1 year
measure: change of QOL-DN
timeFrame: Week3,Week24,Week52
measure: change of EQ-5D-5L
timeFrame: Week 24、Week 52
measure: change of 10-MWT
timeFrame: Week 24、Week 52
measure: change of NFL
timeFrame: Week 3、Week 24、Week 52
measure: change of mBMI
timeFrame: Week 3、Week 24、Week 52
measure: change of NT-proBNP
timeFrame: Week 24、Week 36、Week 52
measure: change of troponin
timeFrame: Week 24、Week 36、Week 52
measure: change of 6-MWT
timeFrame: Week 36、Week 52
measure: change of NYHA cardiac function classification change of NYHA cardiac function classification
timeFrame: Week 24、Week 36、Week 52
measure: change of KCCQ
timeFrame: Week 24、Week 36、Week 52
measure: change of Interventricular septal wall thickness
timeFrame: Week 52
measure: change of Left ventricular (LV) posterior wall thickness
timeFrame: Week 52
measure: change of LV mass
timeFrame: Week 52
measure: change of LV end-diastolic volume
timeFrame: Week 52
measure: change of LV end-systolic volume
timeFrame: Week 52
measure: change of LV ejection fraction
timeFrame: Week 52
measure: change of Cardiac output
timeFrame: Week 52
measure: change of Global longitudinal strain
timeFrame: Week 52
measure: change of Left atrial size
timeFrame: Week 52
measure: change of Left ventricular end-diastolic volume on Cardiac magnetic resonance
timeFrame: W52
measure: change of Left ventricular end-systolic volume on Cardiac magnetic resonance
timeFrame: Week 52
measure: change of Left ventricular systolic volume on Cardiac magnetic resonance
timeFrame: Week 52
measure: change of Left ventricular ejection fraction on Cardiac magnetic resonance
timeFrame: Week 52
measure: change of Overall longitudinal strain of left ventricle on Cardiac magnetic resonance
timeFrame: Week 52
measure: change of Mitral ring plane contraction displacement on Cardiac magnetic resonance
timeFrame: Week 52
measure: change of Left ventricular myocardial mass on Cardiac magnetic resonance
timeFrame: Week 52
measure: change of Right ventricular end-diastolic volume on Cardiac magnetic resonance
timeFrame: Week 52
measure: change of Right ventricular end-systolic volume on Cardiac magnetic resonance
timeFrame: Week 52
measure: change of Right ventricular systolic volume on Cardiac magnetic resonance
timeFrame: Week 52
measure: change of Right ventricular ejection fraction on Cardiac magnetic resonance
timeFrame: Week 52
measure: change of Tricuspid ring plane contraction offset on Cardiac magnetic resonance
timeFrame: Week 52
measure: change of Right ventricular longitudinal strain on Cardiac magnetic resonance
timeFrame: Week 52
measure: change of Left atrial volume on Cardiac magnetic resonance
timeFrame: Week 52
measure: change of Right atrial volume on Cardiac magnetic resonance
timeFrame: Week 52
measure: change of Extracellular volume on Cardiac magnetic resonance
timeFrame: Week 52
measure: level of anti-drug antibody
timeFrame: through study completion, an average of 1 year
measure: level of cas9 antibody
timeFrame: through study completion, an average of 1 year
Other outcomes:
Locations:
city: Hangzhou
country: China
facility: the First Affiliated Hospital, Zhejiang University School of Medicine
state: Zhejiang
Study officials:
References: