Clinical trial NCT02697903: Pomegranate Improve Biological Recovery Kinetics in Elite Weightlifter
Official title: Pomegranate Supplementation Accelerates the Recovery Kinetics of Muscle Damage, Muscle Soreness and Inflammatory Marker After Weightlifting Training Session.
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Healthy
Keywords: Creatine kinase supplementation
C-reactive protein
resistance training
Pomegranate supplementation
pomegranate juice
muscle damage response
Lead sponsor: The Higher Institute of Sport and Physical Education of Sfax
Sponsor class: OTHER
First posted: 2016-03-03
Enrollment: 9
Sex: MALE
Minimum age (years): 20
Maximum age (years): 24
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: NON_RANDOMIZED
Intervention model: CROSSOVER
Primary purpose: SUPPORTIVE_CARE

Brief summary: From the recent scientific literature in the field of biological response to physical exercise, a significant increase in muscle damage, soreness and inflammation were registered immediately and even 48 after resistance exercise. In the other hand, from the recent scientific literature in the field of nutrition it is well established that Pomegranate juice is a potent antioxidant that can help prevent or treat various disease risk factors. However, only few studies associated the effect of the pomegranate with the physical exercise. To the authors' knowledge, there are no studies investigated the acute and delayed effect of pomegranate supplementation in performance and muscle recovery after exercises involved muscle of whole body. Therefore, the aim of the present study was to investigate the effect of natural Pomegranate juice supplementation on the acute and delayed response of muscle soreness and biochemical parameters following weightlifting training session

Detailed description: Participants (i.e., 9 elite weightlifters) performed-as part of their habitual training program from 08h:00 to 09h:45- two training sessions using respectively, PLA and POMj supplementations (500ml), with a recovery period of 48 h in between. Upon arrival for their first test session, each participant's body mass and height were recorded. Moreover, before and after each tested training session, oral temperature was recorded with a calibrated digital clinical thermometer (Omron, Paris, France; accuracy: 0.05°C) inserted sublingually for at least 3 min with the subjects in a seated resting position for at least 15 min. Also, before and after each training session, fasting blood samples (blood sample 2, 3, 4 and 5, Figure1) were collected and heart rate (HR) and systolic arterial pressure (SAP) were recorded using a heart rate monitor and a manual sphygmomanometer. Additionally, to assess the recovery kinetic of the biological parameters, blood sample, temperature, HR and SAP were collected at resting state (i.e., after 10 days of recovery, blood sample 6) and immediately (3min) after the training session which proceed the PLA session (blood sample 1). Given that values of markers of muscle damage remain raised for at least 7 days following intensive weightlifting exercises, a recovery period of 10 days was chosen to evaluate the biological resting state. Additionally, given that (i) using randomized order in this study will results in consuming POMj then PLA supplementations (after 48h) for some participant and (ii) the delayed effect of POMj (which we expect) could alter the results of PLA supplementation, in the present study investigators choose to avoid randomized order and to evaluate the PLA at first then the POMj effect using all the participant together. Supplements (1500ml) of placebo (PLA) or pomegrenate juice (POMj) were taken three times daily in the 48h which proceed the first and the second tested training sessions respectively (i.e. 250ml × 6 times with 8-h intervals between it). Moreover, during these tested sessions, subject consumed an additional 500ml of PLA and 500ml of POMj, respectively. The tested quantity of the natural POMj were prepared from a fresh pomegranate fruit 48h before the beginning of the experimentation and were shipped frozen and stored at -4°C. No additional chemical products were added to the natural POMj. Each 500-mL of the tested POMj contained 2.56g of total polyphenol, 1.08g of ortho-diphenols, 292.59mg of flavonoids and 46.75mg of flavonols. Subjects were reminded verbally through phone communication to consume at the required times their supplements. Placebo juice consisted of an Pomegranate-flavored commercial drink contained water, citric acid, natural flavor and natural identical flavor (Pomegranate), sweeteners (aspartame × (0.3g/l), acesulfame K (0.16g/l)), stabilizers (Arabic gum) and lacked antioxidants, fruit and vegetable extracts or vitamins. Placebo juice contains no polyphenols.

Eligibility criteria: Inclusion Criteria:

* trained at least five sessions per week with 90 to 120 min per session
* had an experience of more than 3 years in Olympic weightlifting

Exclusion Criteria:

* use any antioxidant (e.g., vitamin E, A, C etc..) or anti-inflammatory during the experimentation period and one month before
* had injuries during the experimentation period and one month before

Primary outcomes:
measure: Change in physical performance from using Placebo to using Pomegranate juice
timeFrame: 3min after Placebo and Pomegranate training sessions
measure: Change in Acute Hematological responses from using Placebo to using Pomegranate juice
timeFrame: 3min after Placebo and Pomegranate training sessions
measure: Change in Acute Biochemical responses from using Placebo to using Pomegranate juice
timeFrame: 3min after Placebo and Pomegranate training sessions
measure: Change in Delayed Hematological responses from using Placebo to using Pomegranate juice
timeFrame: 48h after Placebo and Pomegranate training sessions
measure: Change in Delayed Biochemical responses from using Placebo to using Pomegranate juice
timeFrame: 48h after Placebo and Pomegranate training sessions
Secondary outcomes:
measure: Change in the acute fatigue perception (RPE values) from Placebo to Pomegranate supplementation
timeFrame: 3min after Placebo and Pomegranate training sessions
measure: Change in the delayed muscle soreness (DOMS values) from Placebo to Pomegranate supplementation
timeFrame: 48 after Placebo and Pomegranate training sessions
measure: Change in the acute oral temperature response from Placebo to Pomegranate supplementation
timeFrame: 3min after Placebo and Pomegranate training sessions
measure: Change in the acute Heart rate ( HR) response from Placebo to Pomegranate supplementation
timeFrame: 3min after Placebo and Pomegranate training sessions
measure: Change in the acute systolic arterial pressure (SAP) response from Placebo to Pomegranate supplementation
timeFrame: 3min after Placebo and Pomegranate training sessions
measure: Baseline values of body mass
timeFrame: at baseline status
measure: baseline values of height
timeFrame: at baseline status
Other outcomes:
Locations:
city: Sfax
country: Tunisia
facility: Achraf
Study officials:
affiliation: Institute of sport and physical education of Sfax, Tunisia
name: Achraf Ammar, Dr
role: PRINCIPAL_INVESTIGATOR
References:
citation: Ammar A, Trabelsi K, Bailey SJ, Turki M, Bragazzi NL, Boukhris O, El Abed K, Bouaziz M, Ayadi F, Driss T, Souissi N, Chtourou H, Hokelmann A. Effects of natural polyphenol-rich pomegranate juice supplementation on plasma ion and lipid profiles following resistance exercise: a placebo-controlled trial. Nutr Metab (Lond). 2020 Apr 16;17:31. doi: 10.1186/s12986-020-00451-1. eCollection 2020.
citation: Ammar A, MounaTurki, Trabelsi K, Bragazzi NL, Boukhris O, Bouaziz M, Ayadi F, El Abed K, Driss T, Souissi N, Chtourou H, Bailey SJ, Hoekelmann A. Effects of natural polyphenol-rich pomegranate juice on the acute and delayed response of Homocysteine and steroidal hormones following weightlifting exercises: a double-blind, placebo-controlled trial. J Int Soc Sports Nutr. 2020 Mar 6;17(1):15. doi: 10.1186/s12970-020-00345-w.
citation: Ammar A, Turki M, Chtourou H, Hammouda O, Trabelsi K, Kallel C, Abdelkarim O, Hoekelmann A, Bouaziz M, Ayadi F, Driss T, Souissi N. Pomegranate Supplementation Accelerates Recovery of Muscle Damage and Soreness and Inflammatory Markers after a Weightlifting Training Session. PLoS One. 2016 Oct 20;11(10):e0160305. doi: 10.1371/journal.pone.0160305. eCollection 2016.