Clinical trial NCT00827450: Effects of Coffee on Hepatic Steatosis Induced by a High Fructose Diet
Official title: Effects of Coffees With Various Compositions of Antioxidants on Hepatic Steatosis Induced by a High Fructose, Hypercaloric Diet
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Hepatic Steatosis
Keywords: hepatic steatosis
coffee
fructose
lipids
Lead sponsor: University of Lausanne
Sponsor class: OTHER
Collaborators: name: Nestlé Research Center, Vers-chez-les-blanc, Switzerland
First posted: 2009-01-22
Enrollment: 13
Sex: MALE
Minimum age (years): 18
Maximum age (years): 30
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: FACTORIAL
Primary purpose: PREVENTION

Brief summary: This study will assess

* whether coffee consumption protects against fructose-induced hepatic steatosis in healthy humans
* whether the protective effect of coffee is dependent on it's antioxidant composition

Detailed description: Epidemiological studies suggest that coffee consumption improves glucose homeostasis in insulin resistant subjects. An increase in intrahepatic lipids (hepatic steatosis) is highly prevalent in patients with the metabolic syndrome and may be used as a marker of altered hepatic lipid metabolism. Such an increased hepatic lipids content can be experimentally produced in healthy humans by a 6-day high fructose diet.

The purpose of this study is to evaluate whether coffee prevents hepatic lipid deposition in healthy male subjects fed a fructose-rich hypercaloric diet. Both caffeine and antioxidants (yet unspecified) may be involved.. To sort out the role of caffeine and antioxidants, we will test 3 different soluble coffee, ie fully torrefied decaffeinated coffee , partially torrefied decaffeinated coffee, and partially torrefied caffeinated coffee.

Eligibility criteria: Inclusion Criteria:

* BMI between 19 and 15 kg/m2
* less than 30 min physical activity /day
* habitual coffee consumption less than three cupy /day
* consumption of caffeine-containing sodas less than 2 servings/day
* non-smoker

Exclusion Criteria:

* consumption of alcohol more than 40g/day
* presence of metallic foreign bodies
* history of eye surgery
* family history of diabetes mellitus
* history of food intolerance
* vegetarians

Primary outcomes:
measure: intra-hepatocellular lipid (IHCL) concentration
timeFrame: will be measured after 6 days on a hypercaloric, high fructose (4g/kg body weight/day) diet +/- treatement
Secondary outcomes:
measure: fasting plasma triglycerides
timeFrame: will be measured after 6 days on a hypercaloric, high fructose (4g/kg body weight/day) diet +/- treatement
measure: fasting net lipid oxidation
timeFrame: will be measured after 6 days on a hypercaloric, high fructose (4g/kg body weight/day) diet +/- treatement
measure: fasting net carbohydrate oxidation
timeFrame: will be measured after 6 days on a hypercaloric, high fructose (4g/kg body weight/day) diet +/- treatement
measure: whole body ketone bodies turnover and oxidation (13C 3-hydroxybutyrate)
timeFrame: will be measured after 6 days on a hypercaloric, high fructose (4g/kg body weight/day) diet +/- treatement
measure: whole body glucose turnover (6,6 2H2 glucose)
timeFrame: will be measured after 6 days on a hypercaloric, high fructose (4g/kg body weight/day) diet +/- treatement
measure: whole body glycerol turnover (2H5 glycerol)
timeFrame: will be measured after 6 days on a hypercaloric, high fructose (4g/kg body weight/day) diet +/- treatement
Other outcomes:
Locations:
city: Lausanne
country: Switzerland
facility: Centre d'investigations cliniques "cardiomet"/ CHUV
Study officials:
affiliation: Department of Physiology, University of Lausanne, Switzerland
name: Luc Tappy, MD
role: PRINCIPAL_INVESTIGATOR
References:
citation: Lecoultre V, Carrel G, Egli L, Binnert C, Boss A, MacMillan EL, Kreis R, Boesch C, Darimont C, Tappy L. Coffee consumption attenuates short-term fructose-induced liver insulin resistance in healthy men. Am J Clin Nutr. 2014 Feb;99(2):268-75. doi: 10.3945/ajcn.113.069526. Epub 2013 Nov 20.