Clinical trial NCT02843438: Evaluation of Biological Biomarkers Diagnostic of Toxoplasmosis Uveitis
Official title: Evaluation of Biological Biomarkers Diagnostic of Toxoplasmosis Uveitis
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Subjects Clinically Suspected an Active Source of Toxoplasmosis Chorioretinitis Infection
Keywords: Toxoplasmosis
Uveitis
Chorioretinitis
Infection
Lead sponsor: University Hospital, Grenoble
Sponsor class: OTHER
First posted: 2016-07-25
Enrollment: 72
Maximum age (years): 18
Standard ages: CHILD
ADULT
Accepts healthy volunteers: no
Allocation: NA
Intervention model: SINGLE_GROUP
Primary purpose: SCREENING

Brief summary: Toxoplasmosis affects one to two newborn each 10000 births. Among them, 1 to 2 % develop learning disabilities or die, and 4 to 27 % develop a chorioretinitis sometimes leading to an amblyopia responsible for visual impairment. Toxoplasmosis uveitis affects too adults immunocompetent and immunodepressed who have had an acquired toxoplasmosis. Clinical diagnosis of ocular toxoplasmosis is more complicated in presence of posterior neuro-retinitis, inflammation of the papilla, uveitis without chorioretinitis, fuchs heterochromic iridocyclitis, scleritis, diffuse necrotizing or multifocal retinitis. In this situation biological markers diagnostic and prognostic of toxoplasmosis uveitis are useful.

Highly kept molecules (during evolution) like stress proteins (Hsp) are are found in the host and the pathogen and there can trigger a crossed immune response. Stress proteins haven't been explored yet, in the context of toxoplasmosis uveitis on humans.

The hypothesis is that Hsp70 and antibodies anti-Hsp70 are diagnostic and prognostic markers of ocular toxoplasmosis.

The goal is to evaluate diagnosis value of biological markers (Hsp70 and antibodies IgG anti-Hsp70) in toxoplasmosis uveitis.


Eligibility criteria: Inclusion Criteria:

* Age over 18 years
* Subjects clinically suspected at least of one active source of toxoplasmosis chorioretinitis infection
* Persons affiliated to national social security

Exclusion Criteria:

* Pregnant, parturient or breastfeeding women
* Persons deprived of liberty by judicial or administrative decision, person under legal protection
* Refusal by a patient to do the PCA (anterior chamber puncture)
* Patients whose following will be difficult or nonexistent

Primary outcomes:
measure: ELISA tests
timeFrame: About an hour
Secondary outcomes:
Other outcomes:
Locations:
city: La Tronche
country: France
facility: UniversityHospitalGrenoble
Study officials:
affiliation: Grenoble Hospital University
name: Laurence Bouillet, Professor
role: PRINCIPAL_INVESTIGATOR
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