Clinical trial NCT01701479: Long Term Continuous Infusion ch14.18/CHO Plus s.c. Aldesleukin (IL-2)
Official title: A Phase I/II Dose Schedule Finding Study of ch14.18/CHO Continuous Infusion Combined With Subcutaneous Aldesleukin (IL-2) in Patients With Primary Refractory or Relapsed Neuroblastoma
Study type: INTERVENTIONAL
Overall status: UNKNOWN
Phase: PHASE1
PHASE2
Conditions: Neuroblastoma
Keywords: Neuroblastoma
Refractory neuroblastoma
Relapsed neuroblastoma
ch14.18/CHO
Aldesleukin (IL-2)
Lead sponsor: St. Anna Kinderkrebsforschung
Sponsor class: OTHER
Collaborators: name: University Medicine Greifswald
name: St. Anna Children's Hospital, Vienna
name: Hospital Universitario La Fe
name: Istituto Giannina Gaslini
name: Gustave Roussy, Cancer Campus, Grand Paris
name: Schneider Children's Medical Center, Israel
name: Great Ormond Street Hospital for Children NHS Foundation Trust
name: University Hospital, Toulouse
name: Johann Wolfgang Goethe University Hospital
name: Jena University Hospital
name: Children's University Hospital, Ireland
name: University Hospital Tuebingen
name: Fondazione IRCCS Istituto Nazionale dei Tumori, Milano
name: Alder Hey Children's NHS Foundation Trust
name: University Hospitals Bristol and Weston NHS Foundation Trust
name: Newcastle-upon-Tyne Hospitals NHS Trust
name: The Leeds Teaching Hospitals NHS Trust
name: NHS Greater Glasgow and Clyde
name: Medical University of Graz
name: Medical University Innsbruck
name: Centre Leon Berard
name: Hospital Infantil Universitario Niño Jesús, Madrid, Spain
name: University Hospital Southampton NHS Foundation Trust
name: Institut Curie
name: Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico
First posted: 2012-10-05
Enrollment: 288
Sex: ALL
Minimum age (years): 1
Maximum age (years): 21
Standard ages: CHILD
ADULT
Accepts healthy volunteers: no
Allocation: RANDOMIZED
Intervention model: PARALLEL
Intervention model description: The patients will be randomised to immunotherapy with isotretinoin (13-cis-RA) and ch14.18/CHO, with or without aldesleukin (IL-2).
Primary purpose: TREATMENT

Brief summary: The main aim of this clinical trial is to find a way of giving ch14.18/CHO, in combination with subcutaneous aldesleukin (IL-2) and oral isotretinoin (13-cis-RA), to children and young people with primary refractory or relapsed neuroblastoma without intravenous morphine.

Detailed description: Although a lot of children and young people with neuroblastoma can be cured with current standard chemotherapy, sometimes, particularly at relapse the disease no longer responds to standard drugs. Therefore, there is a need to find new drug combinations which will act against neuroblastoma which no longer responds to standard drugs.

Ch14.18/CHO has been shown to improve the outcome of patients with neuroblastoma. However, one of the side effects of receiving ch14.18/CHO is severe pain. High doses of intravenous morphine are needed to control the pain and this means that patients must stay in hospital. Results from other clinical trials have shown that giving ch14.18/CHO over a longer time reduces pain, yet the drug still works just as well to fight the neuroblastoma. The clinical trial aims to give ch14.18/CHO over a longer time so that intravenous morphine is not needed and that this treatment regimen can ultimately be given in an outpatient setting.

Ch14.18/CHO is a monoclonal antibody. Monoclonal antibodies are made in the laboratory and are designed to bind to specific cancer cells. Ch14.18/CHO was designed to bind to neuroblastoma cells and other cancer cells that express the GD-2 antigen. The GD-2 antigen is expressed by virtually all neuroblastoma cells. An antigen is a substance that stimulates an immune response in the body by producing antibodies. Thus, when ch14.18/CHO binds to the neuroblastoma cells, the body's immune system is stimulated to attack and kill the neuroblastoma cells. Ch14.18/CHO is called chimeric, because it was genetically engineered to consist of 30% mouse-protein and of 70% human protein.

Ch14.18/CHO represents a new kind of cancer therapy that, unlike chemotherapy and radiation, targets the destruction of cancer cells without destroying nearby healthy cells. There is laboratory evidence to suggest that ch14.18/CHO can activate the body's own immune cells to destroy cancer cells. These immune cells include killer cells that are activated or stimulated by aldesleukin (IL-2). Therefore this treatment is a combination of ch14.18/CHO and aldesleukin (IL-2).

Aldesleukin (IL-2) is a substance that is similar to a substance made by the body in all individuals. Under normal circumstances, the body makes small amounts of aldesleukin (IL-2) that help white blood cells fight infection. It is now possible to make aldesleukin (IL-2) in the laboratory and give humans much higher doses than their own body makes. There is evidence in the laboratory and in animals that aldesleukin (IL-2) increases the anti-cancer effect of monoclonal antibodies like ch14.18/CHO. We wish to study whether aldesleukin (IL-2) can help improve the effectiveness of ch14.18/CHO in humans.

In addition to ch14.18/CHO and aldesleukin (IL-2), isotretinoin (13-cis-RA) will also be given. Isotretinoin (13-cis-RA) is considered standard treatment for patients with neuroblastoma and works by induction of neuroblastoma cell death.

Eligibility criteria: Inclusion Criteria:

* At study entry patients must be \> 1 year but \<= 21 years of age. NOTE: Patients \>21 years but \<= 45 years of age, fulfilling the remaining criteria, may be enrolled in the study. These patients will be analysed separately and will not be included in the dose finding schedule algorithm. The purpose for inclusion of the older patients is to enable the collection of tolerability data.
* Patients must be diagnosed with neuroblastoma according to the INSS criteria.
* Must have received at least one previous high dose treatment followed by stem cell rescue after conventional therapy.
* Must fulfil one of the following criteria:
* Patients with stage 4 neuroblastoma on the current high-risk SIOPEN trial (HR-NBL-1/SIOPEN) either with primary refractory disease having had more than two front-line treatments or patients ineligible for the R2 randomization due to major delays after completed high-dose treatments.
* Treated and responding relapse after primary stage 4 disease, without signs of progression at study entry
* Treated and responding disseminated relapse after primary localized neuroblastoma without signs of progression at study entry.
* Patients must have a performance status greater or equal 70% (Lansky Score or Karnofsky, see Appendix 1: performance Scales , page 91)
* Patients must have an estimated life expectancy of at least 12 weeks.
* Patients must consent to the placement of a central venous line, if one has not already been placed.
* Patients must be off any standard or experimental treatments for at least two weeks prior to study entry and be fully recovered from the short term major toxic effects.
* Patients must have no immediate requirements for palliative chemotherapy, radiotherapy or surgery.
* At least 4 weeks after major surgery (e.g. laporotomy or thoracotomy) and fully recovered from any post-surgical complications.
* HIV and Hepatitis B negative.
* Females of childbearing potential must have a negative pregnancy test. Patients of childbearing potential must agree to use an effective birth control method. Female patients who are lactating must agree to stop breast-feeding.
* Patients may have had prior CNS metastasis providing the following criteria are all met:
* the patient's CNS disease has been previously treated,
* the patient's CNS disease has been clinically stable for four weeks prior to starting this study (assessment must be made clinically and by CT or MRI scan),
* the patient is off steroids for CNS disease for four weeks prior to starting on study and during the course of the study.
* Patients with seizure disorders may be enrolled if on anticonvulsants and are well controlled.
* All patients and/or their parents or legal guardians must sign a written informed consent
* All institutional and national requirements for human studies must be met.
* Laboratory Testing:
* Patients should have a shortening fraction of \>= 30 % by Echocardiogram.
* Patients should have FEV1 and FVC \>60% of the predicted by pulmonary function tests. Children unable to do PFTs should have no dyspnea at rest and a pulse oximetry \>94% on room air.
* All patients must have adequate bone marrow function as defined by ANC \>1 10\^9/L, platelets \>= 50 10\^9/L and haemoglobin \> 9.0 g/dL.
* Patients must have adequate liver function, as defined by an ALT or AST \< 5 x normal and a total bilirubin \< 1.0 mg/dL.
* Patients must have adequate renal function, as defined by a serum creatinine \<1.5 mg/dL or a creatinine clearance or radioisotope GFR of \> 60 mL/minute/1.73m2.

Exclusion Criteria:

* Patients with progressive disease
* Patients who have previously received treatment with ch14.18/SP2/0 and/or ch14.18/CHO.
* Platelet transfusion dependent.
* Patients with significant intercurrent illnesses and/or any of the following:
* Patients with symptoms of congestive heart failure or uncontrolled cardiac rhythm disturbance.
* Patients with significant psychiatric disabilities or uncontrolled seizure disorders.
* Patients with active infections.
* Patients with a clinically significant neurologic deficit or objective peripheral neuropathy (Grade \>2) are ineligible.
* Patients with clinically significant, symptomatic, pleural effusions.
* Patients who require, or are likely to require, corticosteroid or other immunosuppressive drugs.
* Concurrent treatment with any non-trial anticancer therapies.

Primary outcomes:
measure: Event free survival
timeFrame: through study completion, an average of 1 year
Secondary outcomes:
measure: Pain-toxicity endpoint
timeFrame: through study completion, an average of 1 year
measure: Efficacy endpoint
timeFrame: through study completion, an average of 1 year
measure: Systemic immune modulation/response
timeFrame: through study completion, an average of 1 year
measure: Assessment of absolute lymphocyte counts and absolute NK cell numbers after the respective cycles as a measurement of response to s.c. aldesleukin (IL-2) in the standard treatment arm.
timeFrame: through study completion, an average of 1 year
measure: Determination of pharmacokinetics of ch14.18/CHO by assessing blood levels of ch14.18/CHO via ELISA (Enzyme-linked-Immunosorbent Assay)
timeFrame: through study completion, an average of 1 year
measure: Evaluation of anti-tumour response in patients with measureable disease
timeFrame: through study completion, an average of 1 year
measure: Evaluation of impact of KIR/KIRL mismatch and Fc receptor polymorphisms on EFS (PCR sequence-specific primer technique)
timeFrame: through study completion, an average of 1 year
Other outcomes:
Locations:
city: Vienna
country: Austria
facility: St. Anna Kinderspital
city: Paris
country: France
facility: Institut Curie
city: Villejuif
country: France
facility: Institut Gustave Roussy
city: Greifswald
country: Germany
facility: University Children's Hospital
city: Petach Tikvah
country: Israel
facility: Schneider Children's Medical Centre of Israel
city: Genova
country: Italy
facility: Gaslini Children's Hospital
city: Valencia
country: Spain
facility: Hospital Universitario La Fe
city: Birmingham
country: United Kingdom
facility: Birmingham Children's Hospital NHS Foundation Trust
city: Bristol
country: United Kingdom
facility: University Hospitals Bristol NHS Foundation Trust
city: Leeds
country: United Kingdom
facility: Leeds Teaching Hospitals NHS Trust
city: Liverpool
country: United Kingdom
facility: Alder Hey Children's NHS Foundation Trust
city: London
country: United Kingdom
facility: University College Hospitals NHS Foundation Trust
city: London
country: United Kingdom
facility: Great Ormond Street Hospital for Children NHS Foundation Trust
city: Newcastle
country: United Kingdom
facility: The Newcastle upon Tyne Hospitals NHS Foundation Trust
Study officials:
affiliation: University Medicine Greifswald
name: Holger Lode, MD, PhD
role: PRINCIPAL_INVESTIGATOR
References: