Clinical trial NCT02227043: Study Of Weight Development Over Time
Official title: Study Of Adipose Tissue Function and Weight Development Over Time
Study type: OBSERVATIONAL
Overall status: COMPLETED
Conditions: Obesity
Insulin Resistance
Weight Reduction
Weight Gain
Lead sponsor: Karolinska Institutet
Sponsor class: OTHER
Collaborators: name: Region Stockholm
First posted: 2014-08-27
Enrollment: 105
Sex: ALL
Minimum age (years): 24
Maximum age (years): 90
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: yes
Observational model: COHORT
Time perspective: PROSPECTIVE
Sampling method: NON_PROBABILITY_SAMPLE
Study population: Observational study All subjects that have been previously investigated at our lab at least 7 years ago

Brief summary: The investigators will study the influence of fat cell size/number and adipose function on weight development over very long time periods (years). By comparing fat biopsies obtained at baseline and after \>7 years, the investigators will determine the association between adipose morphology/function and changes in weight or development of cardiometabolic complications (e.g. insulin resistance, type 2 diabetes, dyslipidemia and hypertension).

Detailed description: The investigators will ask all subjects that have previously been examined at the investigators laboratory (from 1992 and forward) in studies of adipose tissue function if they are interested to participate in a new study. The minimal follow up time is 7 years.

Saved blood samples and samples from old fat biopsies will be analyzed together with samples from new examinations.

The investigators plan to examine the subjects in the same way as performed at the baseline examinations. This includes estimation of total body fat using bioimpedance, venous blood samples for determination of insulin, glucose and lipids, subcutaneous fat biopsies with determination of fat cell size/number, lipolysis/lipogenesis as well as analysis of adipokine secretion.

In selected patients, baseline examinations included DEXA-measurement (dual energy x-ray absorptiometry) for determination of body fat distribution as well as hyperinsulinemic euglycemic clamp. In these cases, the same type of examinations will be repeated at their follow up visits.

Eligibility criteria: Inclusion Criteria:

* Participated in previous examination at our lab at least 7 years ago.
* No blood samples or adipose samples saved from last examination

Exclusion Criteria:

* Decline to participate after invitation.
* Serious illness or mental disorder.
* Warfarin or other new oral anticoagulant that increase risk of bleeding.

Primary outcomes:
measure: Weight development over time and correlation to adipose morphology
timeFrame: from 2014 up to 2024, up to 10 years
measure: Weight development and correlation to lipolysis
timeFrame: 2014 to 2024, up to 10 years
measure: Weight development and correlation to lipogenesis
timeFrame: 2014 to 2024, up to 10 years
Secondary outcomes:
measure: adipose tissue morphology development and insulin sensitivity
timeFrame: 2014 to 2024, up to 10 years
measure: adipose morphology and changes in fat mass
timeFrame: 2014 to 2024, up to 10 years
measure: adipose morphology and development of fat mass
timeFrame: 2014 to 2024, up to 10 years
Other outcomes:
Locations:
city: Stockholm
country: Sweden
facility: Lipid laboratory Karolinska University Hospital Huddinge
Study officials:
affiliation: Karolinska Institutet
name: Mikael Ryden, MD, PhD
role: PRINCIPAL_INVESTIGATOR
affiliation: Karolinska Institutet
name: Peter Arner, MD, PhD
role: STUDY_DIRECTOR
affiliation: Karolinska Institutet
name: Daniel P Andersson, MD
role: STUDY_CHAIR
References:
citation: Ryden M, Gao H, Arner P. Influence of Aging and Menstrual Status on Subcutaneous Fat Cell Lipolysis. J Clin Endocrinol Metab. 2020 Apr 1;105(4):dgz245. doi: 10.1210/clinem/dgz245.
citation: Arner P, Andersson DP, Backdahl J, Dahlman I, Ryden M. Weight Gain and Impaired Glucose Metabolism in Women Are Predicted by Inefficient Subcutaneous Fat Cell Lipolysis. Cell Metab. 2018 Jul 3;28(1):45-54.e3. doi: 10.1016/j.cmet.2018.05.004. Epub 2018 May 31.