Clinical trial NCT03613701: Relationship Between Endothelial Progenitor Cells and Revascularization Effect of Moyamoya Disease
Official title: Relationship Between Endothelial Progenitor Cells and Revascularization Effect of Moyamoya Disease
Study type: OBSERVATIONAL
Overall status: UNKNOWN
Conditions: Moyamoya Disease
Keywords: Moyamoya disease
Endothelial progenitor cells
revascularization
Lead sponsor: Affiliated Hospital to Academy of Military Medical Sciences
Sponsor class: OTHER
First posted: 2018-08-03
Enrollment: 120
Sex: ALL
Minimum age (years): 18
Maximum age (years): 60
Standard ages: ADULT
Accepts healthy volunteers: yes
Observational model: CASE_CONTROL
Time perspective: PROSPECTIVE
Sampling method: PROBABILITY_SAMPLE
Study population: Health volunteers' inclusion criteria:

1. Age between 18-60;
2. Male or female;

Exclusion criteria:

Exclude the volunteers with history of cerebrovascular disease and heart disease.

Brief summary: Moyamoya disease is a chronic cerebrovascular disease，The typical pathological manifestations are the stenosis or occlusion of the distal internal carotid artery and/or middle cerebral artery, and the proximal anterior cerebral artery. Meanwhile, the abnormal vascular net, which is the smokey vessel, occurs at the bottom of the brain. Currently the pathogenesis of this disease is unknown. Limited studies have reported the expression of endothelial progenitor cells (EPCs) in moyamoya disease, but the results were inconsistent. Some investigators believe that the number of EPCs in peripheral blood of patients with moyamoya disease is increased, while others believe that the number of EPCs in peripheral blood of moyamoya patients is reduced. Therefore, the investigators need to find a more accurate detection method to confirm the growth of EPC in patients with moyamoya disease. At the same time, whether there is endothelial injury in patients with smoke disease, and the expression of endothelial cells (CEC) in patients with smoke disease, there is no research on this aspect at home and abroad.

Detailed description: Objective: Detect the expression of endothelial progenitor cells and endothelial cells from peripheral blood of patients with moyamoya disease, and to assess the relationship between clinical characteristics.

Design: A single center study, and planned to enroll 120 patients. The present study was to detect the quantities of EPC from peripheral blood in Moyamoya disease by flow cytometry, and to identify the relationship of endothelial progeIlitor cells and effect of the revascularization on Moyamoya disease. The present study also use cerebral ischemia animal model foe intervention experiment, to explore whether EPC can promote vascular remodeling effect of ischemic cerebrovascular disease, and to provide new thought for the treatment of chronic cerebrovascular disorder.

Eligibility criteria: Inclusion Criteria:

1. Whole-brain vessels angiography or magnetic resonance arteriography (MRA) has the following manifestations: stenosis or occlusion of terminal internal carotid artery or the anterior cerebral artery and/or initiating middle cerebral artery; In the arterial phase, the abnormal smokey vascular net near the occlusive or stenosis lesion can be seen.
2. For patients with stable stroke, there was no acute or subacute cerebral infarction or cerebral hemorrhage, and at least 3 months before the last cerebral infarction or cerebral hemorrhage events.

Exclusion Criteria:

1. Exclude atherosclerosis, autoimmune diseases, meningitis, intracranial tumors, multiple neurofibromatosis, Down syndrome, craniocerebral trauma, radiation injury, and other underlying diseases that may cause smoke.
2. Acute or subacute cerebral infarction or cerebral hemorrhage were excluded.

Primary outcomes:
measure: Expression of endothelial progenitor cells and endothelial cells in peripheral blood
timeFrame: 2017.9-2018.8
Secondary outcomes:
Other outcomes:
Locations:
city: Beijing
country: China
facility: The 307th Hospital of Military Chinese People's Liberation Army
state: Beijing
Study officials:
affiliation: The 307th Hospital of Military Chinese People's Liberation Army
name: Lian Duan, Chief
role: STUDY_CHAIR
References: