Clinical trial NCT03794050: Comparing Acute Aerobic and Resistance Exercise
Official title: Effects of Age, Fitness Level, and Modality on Physiological Response to Acute Exercise
Study type: INTERVENTIONAL
Overall status: TERMINATED
Phase: NA
Conditions: Exercise Physiology
Lead sponsor: University of Houston
Sponsor class: OTHER
First posted: 2019-01-04
Enrollment: 35
Sex: ALL
Minimum age (years): 18
Maximum age (years): 75
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: CROSSOVER
Primary purpose: SUPPORTIVE_CARE

Brief summary: The purpose of this study is to determine the effect of age and fitness level on the physiological response to an acute bout of resistance or aerobic exercise.

Detailed description: Young and middle-aged to older adults who are physically active or not physically active will participate in two moderate to vigorous intensity exercise bouts: aerobic exercise and resistance training exercise. Blood will be collected to assess immune function and metabolism.

Eligibility criteria: Inclusion Criteria:

* "Young" Adults: ages 18-30
* "Older" Adults: ages 55-75 and if a female postmenopausal
* Participants classified as "trained" must participate in at least 30 minutes of moderate to vigorous intensity cardiovascular activity at least 3 days per week and engage in resistance training for all of the major muscle groups approximately 2 days / week. They must have upheld this activity for the past 3 months
* Participants classified as "untrained" must be participating in less than 30 minutes of moderate to vigorous intensity cardiovascular activity and less than 2 days of resistance training per week for at least the last 3 months.

Exclusion Criteria:

* Have any contraindications to moderate to vigorous exercise
* Range of motion restrictions that would prevent them from participating in aerobic or resistance training with proper form (they must be ambulatory)
* aking medications (prescription or over the counter) known to influence immune function, including daily NSAID's, beta blockers, statins, bisphosphonates, or steroids.
* Have known cardiovascular, respiratory, metabolic, or renal disease, with the exception of controlled hypertension (as defined by resting BP below 140/90) and/or controlled asthma (self-reported).
* Be pregnant
* Be unable to complete all visits (i.e. must not be planning to leave the Houston area long-term before concluding their participation in the study which will span several weeks)
* Fall outside of a BMI range of (18.5 - 30)
* Consume alcohol or recreational drugs for 24h prior to visits
* Older women cannot be pre-menopausal (must have not had a menses for at least 12 months)
* Unable to speak or read English
* Participants must not have scheduling conflicts that would prevent them from reporting to the laboratory of integrated physiology 4 times over the course of the study

Primary outcomes:
measure: Monocyte subset and function
timeFrame: Pre-resistance training (RT) exercise, change from pre-RT exercise to immediately post-RT exercise, change from pre-RT exercise to 1hr post-RT exercise, change from immediately post-RT exercise to 1hr post-RT exercise
measure: Monocyte subset and function
timeFrame: Pre-aerobic training (AT) exercise, change from pre-AT exercise to immediately post-AT exercise, change from pre-AT exercise to 1hr post-AT exercise, change from immediately post-AT exercise to 1hr post-AT exercise
measure: Monocyte subset and function
timeFrame: compare pre-RT to pre-AT
measure: Monocyte subset and function
timeFrame: compare immediately post-RT to immediately post-AT
measure: Monocyte subset and function
timeFrame: compare 1hr post-RT to 1hr post-AT
measure: NK cell function
timeFrame: Pre-resistance training (RT) exercise, change from pre-RT exercise to immediately post-RT exercise, change from pre-RT exercise to 1hr post-RT exercise, change from immediately post-RT exercise to 1hr post-RT exercise
measure: NK cell function
timeFrame: Pre-aerobic training (AT) exercise, change from pre-AT exercise to immediately post-AT exercise, change from pre-AT exercise to 1hr post-AT exercise, change from immediately post-AT exercise to 1hr post-AT exercise
measure: NK cell function
timeFrame: compare pre-RT to pre-AT
measure: NK cell function
timeFrame: compare immediately post-RT to immediately post-AT
measure: NK cell function
timeFrame: compare 1hr post-RT to 1hr post-AT
measure: Circulating relative and absolute counts of T cell subsets
timeFrame: Pre-resistance training (RT) exercise, change from pre-RT exercise to immediately post-RT exercise, change from pre-RT exercise to 1hr post-RT exercise, change from immediately post-RT exercise to 1hr post-RT exercise
measure: Circulating relative and absolute counts of T cell subsets
timeFrame: Pre-aerobic training (AT) exercise, change from pre-AT exercise to immediately post-AT exercise, change from pre-AT exercise to 1hr post-AT exercise, change from immediately post-AT exercise to 1hr post-AT exercise
measure: Circulating relative and absolute counts of T cell subsets
timeFrame: compare pre-RT to pre-AT
measure: Circulating relative and absolute counts of T cell subsets
timeFrame: compare immediately post-RT to immediately post-AT
measure: Circulating relative and absolute counts of T cell subsets
timeFrame: compare 1hr post-RT to 1hr post-AT
Secondary outcomes:
measure: Serum cortisol
timeFrame: Pre-resistance training (RT) exercise, change from pre-RT exercise to immediately post-RT exercise, change from pre-RT exercise to 1hr post-RT exercise, change from immediately post-RT exercise to 1hr post-RT exercise
measure: Serum cortisol
timeFrame: Pre-aerobic training (AT) exercise, change from pre-AT exercise to immediately post-AT exercise, change from pre-AT exercise to 1hr post-AT exercise, change from immediately post-AT exercise to 1hr post-AT exercise
measure: Serum cortisol
timeFrame: compare pre-RT to pre-AT
measure: Serum cortisol
timeFrame: compare immediately post-RT to immediately post-AT
measure: Serum cortisol
timeFrame: compare 1hr post-RT to 1hr post-AT
Other outcomes:
Locations:
city: Houston
country: United States
facility: University of Houston
state: Texas
Study officials:
affiliation: University of Houston
name: Melissa Markofski, PhD
role: PRINCIPAL_INVESTIGATOR
References:
citation: Graff RM, Jennings K, LaVoy ECP, Warren VE, Macdonald BW, Park Y, Markofski MM. T-cell counts in response to acute cardiorespiratory or resistance exercise in physically active or physically inactive older adults: a randomized crossover study. J Appl Physiol (1985). 2022 Jul 1;133(1):119-129. doi: 10.1152/japplphysiol.00301.2021. Epub 2022 May 26.