Clinical trial NCT01391884: Elimination of Incretin Hormones in Patients With Severe Kidney Failure
Official title: Elimination and Biodegradation of the Incretin Hormones GLP-1 and GIP in Patients With End-stage Renal Disease
Study type: OBSERVATIONAL
Overall status: COMPLETED
Conditions: Uremia
Keywords: Incretin hormones
GLP-1
GIP
Uremia
Dialysis
Lead sponsor: Rigshospitalet, Denmark
Sponsor class: OTHER
First posted: 2011-07-12
Enrollment: 24
Sex: ALL
Minimum age (years): 18
Maximum age (years): 90
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: yes
Observational model: CASE_CONTROL
Time perspective: CROSS_SECTIONAL
Sampling method: NON_PROBABILITY_SAMPLE
Study population: 1. Hemodialysis patients
2. Healthy control subjects

Brief summary: The prevalence of type 2 diabetes (T2D) is increasing rapidly worldwide. T2D is characterized by a severely impaired incretin effect. The incretin effect refers to the insulinotropic action of the nutrient-released incretin hormones glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic peptide (GIP). The incretin effect is defined as the difference in insulin secretory responses between oral and isoglycaemic intravenous glucose challenges (OGTT and IIGI, respectively) and in healthy individuals it accounts for as much as 70% of the insulin response following oral glucose, whereas patients with T2D exhibit an incretin effect in the range of 0 to 30%. Patients with T2D and non-diabetic patients with severe kidney failure share several pathophysiological characteristics, including decreased insulin sensitivity, fasting hyperinsulinaemia and impaired beta-cell function. The reason for these findings remains to be fully elucidated. An ongoing study in our research group is investigating the incretin effect and the incretin hormone secretory responses following OGTT, IIGI and meal ingestion, respectively. In continuation of this study, essential knowledge of metabolism of incretin hormones in an uremic milieu will be obtained in the present study prior to evaluation of the use of incretin-based agents in patients with impaired kidney function. In this second study we evaluate the elimination and biodegradation of GLP-1 and GIP. The biological active incretin hormones are rapidly degraded by the ubiquitous enzyme dipeptidyl peptidase-4 (DPP-4), generating inactive metabolites. The active hormones are however also eliminated by renal clearance, although the importance of this remains questionable. It is likely that the degradation and elimination of the active hormones will be significantly affected in patients with severe kidney impairment.

We hypothesize that elimination and biodegradation of the two incretin hormones, both in it´s active and inactive forms, will be affected in non-diabetic patients with severe kidney failure.


Eligibility criteria: 1)

Inclusion Criteria:

* Male or female; aged 18-90 years
* CKD stage 5 in chronic maintenance dialysis treatment
* BMI: 18,5-28 kg/m2
* Normal fasting plasma glucose (\<6,1 mM)
* Normal or impaired glucose tolerance (PG120 min \<11,1 mM following OGTT)

Inclusion Criteria:

* Male or female; aged 18-90 years
* Healthy including normal kidney function
* BMI: 18,5-28 kg/m2
* Normal fasting plasma glucose (\<6,1 mM)
* Normal or impaired glucose tolerance (PG120 min \<11,1 mM following OGTT)

1+2)

Exclusion Criteria:

* Diabetes mellitus
* Chronic pancreatitis / previous acute pancreatitis
* Treatment with oral glucocorticoids, calcineurin inhibitors, thiazides, dipeptidyl peptidase 4 (DPP4) inhibitors or other drugs, which could interfere with glucose or lipid metabolism
* Inflammatory bowel disease
* Malignant disease
* Bowel resection
* Severe anemia (hemoglobin \<6.5 mmol/L)

Primary outcomes:
measure: Intact GLP-1 concentration
timeFrame: -60 min - 180 min
measure: Total GLP-1 concentration
timeFrame: -60 min - 180 min
measure: Intact GIP concentration
timeFrame: - 60 min - 180 min
measure: Total GIP infusion
timeFrame: -60 min - 180 min
Secondary outcomes:
Other outcomes:
Locations:
city: Copenhagen
country: Denmark
facility: Department of Nephrology P, Copenhagen University Hospital, Rigshospitalet
Study officials:
References:
citation: Idorn T, Knop FK, Jorgensen MB, Christensen M, Holst JJ, Hornum M, Feldt-Rasmussen B. Elimination and degradation of glucagon-like peptide-1 and glucose-dependent insulinotropic polypeptide in patients with end-stage renal disease. J Clin Endocrinol Metab. 2014 Jul;99(7):2457-66. doi: 10.1210/jc.2013-3809. Epub 2014 Apr 8.