Clinical trial NCT03615391: Predictive Role and HuR Mechanisms of Regulation in the Brain Tumours
Official title: Predictive Role and HuR Mechanisms of Regulation in the Brain Tumours
Study type: OBSERVATIONAL
Overall status: UNKNOWN
Conditions: Brain Tumours
Lead sponsor: Central Hospital, Nancy, France
Sponsor class: OTHER
First posted: 2018-08-03
Enrollment: 140
Sex: ALL
Minimum age (years): 18
Standard ages: ADULT
OLDER_ADULT
Observational model: COHORT
Time perspective: RETROSPECTIVE
Sampling method: NON_PROBABILITY_SAMPLE
Study population: Patient with braim tumor (meningioma, glioma, glioblastoma)

Brief summary: The HuR protein binds to AU-rich elements in the untranslated 3' region of messenger RNA, thus allowing their stabilization. Its targets include multiple cell cycle regulating proteins, cytokines and growth factors. In some cancers, its overall expression level but especially its cytoplasmic expression are correlated to a higher grade and constitute a poor prognostic factor. To date, HuR's deregulation mechanisms remain poorly understood. A few experimental studies have shown the role of certain microARNS, or of post-translational modifications. In brain tumours, HuR expression, its prognostic value and its deregulation mechanisms have been little studied to date.

The first part of the project will be a monocentric retrospective study of human brain tumour samples collected during biopsies or surgical removal. We will first evaluate HuR expression in 140 brain tumors, including 40 meningiomas and 100 gliomas of increasing grade, and look for a correlation with histological grade and survival. We will then apprehend the consequences of its deregulation by analyzing different factors involved in the cell cycle and stress response markers. Finally, we will study the mechanisms of HuR deregulation by analyzing the expression level of several microRNAs (miR16, miR519) and the methylation state of HuR.

The second part of the project will focus on cell lines from human brain tumours. We will first attempt to confirm the interactions between HuR and markers involved in the cell cycle and stress response, then the regulation of HuR by its methylation and by microRNAs (miR16 and miR519). We would also like to study the consequences of HuR inhibition and overexpression on cell proliferation, under various conditions of induced stress (pharmacological agents, physical stress). Finally, we will study the consequences of an experimental vitamin B12 deficiency on HuR expression and tumor cell adaptation to stress.


Eligibility criteria: Inclusion Criteria

* Grade I and II meningiomas (WHO)
* Diffuse grade II glioma (astrocytoma, oligodendroglioma)
* Grade III anaplastic gliomas (astrocytomas and anaplastic oligodendrogliomas)
* Grade IV glioblastoma

Primary outcomes:
measure: level HuR's immunohistochemical expression
timeFrame: at diagnosis
measure: time progression-free
timeFrame: through study completion, an average 3 years
measure: time overall survival
timeFrame: through study completion, an average 3 years
Secondary outcomes:
measure: methyl-HuR level
timeFrame: 1 day at diagnosis
measure: PHH3 level
timeFrame: 1 day at diagnosis
measure: MCM6 level
timeFrame: 1 day at diagnosis
measure: Ki-67 level
timeFrame: 1 day at diagnosis
measure: cyclin D1 level
timeFrame: 1 day at diagnosis
measure: Bcl-2
timeFrame: 1 day at diagnosis
measure: pPERK level
timeFrame: 1 day at diagnosis
measure: ATF6 level
timeFrame: 1 day at diagnosis
measure: SIRT1 level
timeFrame: 1 day at diagnosis
measure: IRE-1α level
timeFrame: 1 day at diagnosis
measure: HIF-1α level
timeFrame: 1 day at diagnosis
measure: caspase 3 activated level
timeFrame: 1 day at diagnosis
measure: VEGF level
timeFrame: 1 day at diagnosis
measure: CARM1 level
timeFrame: 1 day at diagnosis
measure: miR16 expression level by qRT-PCR
timeFrame: 1 day at diagnosis
measure: miR519 expression level by qRT-PCR
timeFrame: 1 day at diagnosis
Other outcomes:
Locations:
city: Vandoeuvre Les Nancy
country: France
facility: Guillaume GAUCHOTTE
Study officials:
References: