Clinical trial NCT03621891: Alterations in Subgingival Microbiota and Hypoxia in Occlusal Trauma
Official title: Alterations in Subgingival Microbiota and Hypoxia in Occlusal Trauma
Study type: OBSERVATIONAL
Overall status: COMPLETED
Conditions: Chronic Periodontitis
Occlusal Trauma
Keywords: hyper-occlusion
hypoxia
occlusal trauma
periodontitis
subgingival microbiota
Lead sponsor: Tokat Gaziosmanpasa University
Sponsor class: OTHER
First posted: 2018-08-09
Enrollment: 80
Sex: ALL
Minimum age (years): 29
Maximum age (years): 42
Standard ages: ADULT
Accepts healthy volunteers: yes
Observational model: CASE_CONTROL
Time perspective: CROSS_SECTIONAL
Sampling method: PROBABILITY_SAMPLE
Study population: The study population consisted of 80 participants and 4 study groups were created as follows:

Group 1; healthy individuals without occlusal trauma (Group 1, n=20), 6 men and 14 women, mean age 32.06±2.90 Group 2; periodontitis patients without occlusal trauma (Group 2, n=20), 6 men and 14 women, mean age 36.90±4.67 Group 3; healthy individuals with occlusal trauma (Group 3, n=20), 5 men and 15 women, mean age 34.89±7.51 Group 4; periodontitis patients with occlusal trauma (Group 4, n=20) 7 men and 13 women, mean age 36.76±4.40.

Brief summary: Present study suggests that excessive occlusal forces did not cause a significant change in hypoxia and vascular markers, however a slight alteration in subgingival microbiota was observed.

Detailed description: Occlusal trauma is defined as tissue destruction and/or injury resulting in tissue changes in the periodontium as a consequence of excessive occlusal forces or hyper-occlusion. Previous studies suggested accelerated periodontal destruction around teeth with occlusal trauma and increased mobility, however, the evidence is lacking. Recent animal studies found that occlusal trauma increased osteoclastic activity and alveolar bone loss. One possible mechanism suggested for the progression of periodontal destruction with occlusal trauma is that tooth mobility creates a subgingival environment conducive to overgrowth by periodontal pathogens. However, the association of occlusal trauma and subgingival microbiota was not evaluated in previous studies. Therefore, present study aimed to evaluate subgingival dental plaque components and compare with healthy individuals and periodontitis patients without occlusal trauma.

Eligibility criteria: Inclusion Criteria:

* Systemical health
* No drug use
* No pregnancy or lactation For group 3 and 4;
* Bruxism at least for 2 years
* Occlusal trauma
* Existence of at least 20 functioning teeth

Exclusion Criteria:

* Smoking
* Pregnancy/lactation
* Systemical disease
* Drug use
* Absence of at least 20 functioning teeth

Primary outcomes:
measure: Determination of certain bacterial species in subgingival dental plaque
timeFrame: A day after initial examination
Secondary outcomes:
measure: hypoxic changes in gingiva and serum
timeFrame: One day after initial examination
Other outcomes:
Locations:
city: Tokat
country: Turkey
facility: Gaziosmanpasa University Faculty of Dentistry
city: Tokat
country: Turkey
facility: Gaziosmanpasa University
Study officials:
affiliation: Gaziosmanpasa University
name: HATICE BALCI YUCE, PhD
role: PRINCIPAL_INVESTIGATOR
References: