Clinical trial NCT03807674: MMP-9 Expression in Permanent Mature Teeth With Symptomatic Pulpitis
Official title: A Prospective Clinical Pilot Study of MMP-9 Expression and Short-term Outcome of Coronal Pulpotomy in Mature Permanent Teeth With Symptomatic Pulpitis
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Pulpitis
Keywords: Calcium-silicate, coronal pulpotomy, MMP-9, symptomatic pulpitis.
Lead sponsor: Lithuanian University of Health Sciences
Sponsor class: OTHER
First posted: 2019-01-17
Enrollment: 20
Sex: ALL
Minimum age (years): 14
Maximum age (years): 33
Standard ages: CHILD
ADULT
Accepts healthy volunteers: yes
Allocation: NON_RANDOMIZED
Intervention model: PARALLEL
Primary purpose: TREATMENT

Brief summary: Matrix metallopeptidase-9 (MMP-9) expression was compared in healthy and inflamed pulp and the outcome of coronal pulpotomy in teeth with symptomatic pulpitis was assessed. After procedure blood samples were examined using Elisa kit.

Detailed description: Introduction: Widely used diagnostic tests do not provide information to distinguish the pulp inflammation stage while improvement of materials, techniques and vital pulp therapy have received a wider acceptance in symptomatic pulpitis treatment. The aim of this clinical study was to compare MMP-9 expression in healthy and inflamed pulp and to assess the short-term outcome of coronal pulpotomy in mature permanent teeth with symptomatic pulpitis.

Methods: Patients diagnosed with symptomatic pulpitis were included in this clinical study. Coronal pulpotomy was performed using calcium-silicate based material and blood samples were taken. Initial and postoperative pain was recorded by Heft-Parker visual analog scale at 24 hours and 72 hours after the procedure. In control group teeth with healthy pulp were used. During follow-up visits after three and six-months, patients were examined clinically and radiographically. The ELISA kit was used to determine the levels of MMP-9 in inflamed and healthy coronal pulp tissue.

Eligibility criteria: Inclusion Criteria:

* Teeth diagnosed with symptomatic pulpitis
* Patients who did not use any NSAIDs before the treatment
* Positive cold test of investigated teeth
* Clinically dental caries in contact with pulp chamber
* Permanent teeth with radiographically closed root apex

Exclusion Criteria:

* Patients who refuse to participate in the study
* Medically compromised patients (with immunosuppressive/systemic diseases, patients on medications)
* Teeth with periapical radiolucency or clinical signs of apical periodontitis
* Periodontological compromised teeth (probing depth ≥4mm)
* Internal/external root resorption in periapical radiograph
* Pulp chamber and/or root canal calcification in periapical radiograph
* Teeth with unrestorable crown
* Teeth with a negative response to cold test

Primary outcomes:
measure: MMP-9 amount measurement using ELISA kit
timeFrame: All samples were measured in one time (during 12 hours)
Secondary outcomes:
Other outcomes:
Locations:
city: Kaunas
country: Lithuania
facility: Lithuanian University of Health Science
Study officials:
affiliation: Lithuanian University of Health Science
name: Greta Lodiene
role: STUDY_DIRECTOR
References: