Clinical trial NCT04037826: L. Reuteri DSM 17938 and L. Reuteri ATCC PTA 6475 in Moderate to Severe Irritable Bowel in Adults
Official title: Randomized Clinical Trial on the Safety and Efficacy of L. Reuteri DSM 17938 and L. Reuteri ATCC PTA 6475 in the Treatment of Moderate to Severe Irritable Bowel Syndrome in Adults. Version 1.0, CSUB 0137, 27Jun2017
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Irritable Bowel Syndrome
Keywords: irritable bowel syndrome, adults, L. reuteri
Lead sponsor: Innovacion y Desarrollo de Estrategias en Salud
Sponsor class: OTHER
Collaborators: name: BioGaia AB
First posted: 2019-07-30
Enrollment: 140
Sex: ALL
Minimum age (years): 18
Maximum age (years): 65
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: no
Allocation: RANDOMIZED
Intervention model: PARALLEL
Intervention model description: Randomized, double blind, controlled clinical trial, with two branches. Randomization will be centralized performed by sponsor. L. reuteri Gastrus at dose of 2x108 Colony Forming Units (CFU) or placebo will be adminsitrated twice per day
Primary purpose: TREATMENT

Brief summary: Randomized, double blind, controlled clinical trial, to evaluate safety and efficacy of L. reuteri DSM 17938 and L. reuteri ATCC PTA 6475 as adjuvant to reduce the severity of symptoms in adults with moderate to severe irritable bowel syndrome (IBS). Primary outcome: Global clinical Improve assessed by the GSRS-IBS score. Adults 18-65 years, any gender, with ROME IV criteria for moderate to severe IBS and body max index \<36 will be included. Prescreening will be run for 2 weeks, the intervention period will be 2/days for 14t weeks, follow by a 2 weeks period of observation with no intervention. Secondary outcomes include: a) Improvements in the stool patterns evaluated through Bristol Stool Form (BSF); b) Improvement of the quality of life (QoL) measured by Latin-American IBS-QoL questionnaire; c) Improvement of the depression and anxiety severity evaluated by the Goldberg Depression and Anxiety Scale; d) Frequency of rescue medication use and e) Frequency of Adverse Events (AEs)

Detailed description: Rationale: Irritable bowel syndrome (IBS) is the most commonly diagnosed functional gastrointestinal disorder observed in older children and adults. It is a frequent cause of chronic abdominal pain and changed bowel habits. Produce a significant negative effect on quality of life and it is associated with a significant economic burden disease con total expenditures in USA exceeding $20 billion/year. Since 2008 different clinical trials and meta-analysis had been published about the safety and efficacy of the use of probiotics in adults with IBS. In LATAM evidence about this topic is scarce.

Primary outcome: Global Clinical Improvement assessed by the GSRS-IBS score (Spanish/LA version) Secondary/Exploratory outcomes: Improvements in the Bristol Stool Form (BSF), Quality of Life (QoL) improvement assessed by Latin-American IBS-QoL questionnaire, Goldberg scale depression and Anxiety scale, Frequency of rescue medication use andSecondary adverse events Clinical design: Randomized, double blind, controlled clinical trial, with two arms Type of patients: Age 18-65 years, any gender, ROME IV diagnostic criteria for IBS (Recurrent abdominal pain, on average, at least 1 day per week in the last 3 months, associated with 2 or more of the following criteria a) Related to defecation, b) Associated with a change in frequency of stool, c) Associated with a change in form (appearance) of stool and/or d) Criteria fulfilled for the last 3 months with symptom onset at least 6 months before diagnosis), with a baseline GSRS-IBS score (Spanish/LA version) moderate to severe IBS, body mass index (BMI 25 to 35 (Mild to moderate obesity) and signed Informed consent.

Subject Information and Consent: All subjects will receive written and verbal information regarding the study at Visit 1. This information will emphasise that participation in the study is voluntary and that the subject may withdraw from the study at any time and for any reason. All subjects will be given the opportunity to ask questions about the study and will be given sufficient time to decide whether to participate in the study. Before any study-related procedures, the informed consent form will be signed and personally dated by the subject and by the person who conducted the informed consent discussion. A copy of the subject information including the signed consent form will be provided to the subject.

Interventions: 2 weeks run in, 14 weeks' treatment and 2 weeks follow up. L. reuteri Gastrus (L. reuteri DSM 17938 and L. reuteri ATCC PTA 6475) at dose of 2x108 Colony Forming Units (CFU). One chewable tablet is to be taken twice per day (one in the morning and one in the afternoon) giving a total daily dose of at least 4x108 CFU/day. The placebo will have identical ingredients except for lacking the bacteria. One chewable tablet is to be taken twice per day (one in the morning and one in the afternoon). The study products shall be kept refrigerated (+20C - +80C) during the study. Randomization will be performed by Sponsor Labelling will be performed in Research centres by independent pharmacist or similar person not involved in the study.

Study logistics: After informed consent, subjects must to fill a 2-week pre-randomization form to be sure they fill the criteria for IBS and define severity. If the patient fulfils all inclusion and none of the exclusion criteria the patient will be randomized to one of the two treatment groups. According to the randomization groups the patient will receive the corresponding blinded study product. The patient will be standardized to take the research product twice per day for 14 weeks, and complete the GSRS-IBS (Spanish/LA version) and the BSF on a daily basis, the Latin-American IBS-QoL questionnaire, the Goldberg depression and anxiety scale once, a diet history (2 days/week) and the report of adverse events. A basal faecal sample will be obtained in a subsample of patients to ultra-freeze and measure in a second-time microbiota by pyroseq and faecal calprotectin (At the end of treatment). At the end of treatment subjects will be evaluated according to primary and secondary/exploratory outcomes and they will be invited for a third periods, for 2 weeks with no intervention to evaluate the same clinical parameters.

Eligibility criteria: Inclusion Criteria:

* Age 18-65 years
* Any gender
* ROME IV diagnostic criteria for IBS
* A baseline GSRS-IBS score (Spanish/LA version) moderate to severe IBS
* Body Mass Index (BMI) 25 to 35
* Patient capable of conforming to the protocol
* Signed Informed consent

Exclusion Criteria:

* Patients with relevant systemic, organic or metabolic diseases
* Patients with abnormal laboratory values that could be relevant to the outcome of study treatment
* Previous recent major abdominal surgery
* Consumption of antibiotics, Proton-Pump Inhibitors, H2-antagonists or dietary supplements containing Lactobacillus reuteri within 2 weeks prior to base-line
* Pregnancy

Primary outcomes:
measure: Global clinical improvement
timeFrame: 16 weeks
Secondary outcomes:
measure: Improve on Stool Consistency
timeFrame: 16 weeks
measure: Improve on Quality of Life
timeFrame: 16 weeks
measure: Improve on Depression
timeFrame: 16 weeks
measure: Use of rescue medication
timeFrame: 16 weeks
measure: Adverse events
timeFrame: 16 weeks
Other outcomes:
Locations:
city: Santiago de Chile
country: Chile
facility: Instituto de Nutricion y Tecnologia de los Alimentos
city: Mexico city
country: Mexico
facility: Hospital General Dr. Manuel Gea Gonzalez
Study officials:
References:
citation: McFarland LV, Dublin S. Meta-analysis of probiotics for the treatment of irritable bowel syndrome. World J Gastroenterol. 2008 May 7;14(17):2650-61. doi: 10.3748/wjg.14.2650.
citation: Moayyedi P, Ford AC, Talley NJ, Cremonini F, Foxx-Orenstein AE, Brandt LJ, Quigley EM. The efficacy of probiotics in the treatment of irritable bowel syndrome: a systematic review. Gut. 2010 Mar;59(3):325-32. doi: 10.1136/gut.2008.167270. Epub 2008 Dec 17.
citation: Tiequn B, Guanqun C, Shuo Z. Therapeutic effects of Lactobacillus in treating irritable bowel syndrome: a meta-analysis. Intern Med. 2015;54(3):243-9. doi: 10.2169/internalmedicine.54.2710.
citation: Didari T, Mozaffari S, Nikfar S, Abdollahi M. Effectiveness of probiotics in irritable bowel syndrome: Updated systematic review with meta-analysis. World J Gastroenterol. 2015 Mar 14;21(10):3072-84. doi: 10.3748/wjg.v21.i10.3072.
citation: Zhang Y, Li L, Guo C, Mu D, Feng B, Zuo X, Li Y. Effects of probiotic type, dose and treatment duration on irritable bowel syndrome diagnosed by Rome III criteria: a meta-analysis. BMC Gastroenterol. 2016 Jun 13;16(1):62. doi: 10.1186/s12876-016-0470-z.