Clinical trial NCT03717948: Examining the Impact of Blood Flow Restricted Aerobic Exercise on PGC-1α mRNA Expression in Young Healthy Males
Official title: Examining the Impact of Acute Blood Flow Restricted Aerobic Exercise on PGC-1α mRNA Expression in Young Healthy Males: A Randomized Cross-over Trial
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Mitochondrial Biogenesis
Keywords: Acute Exercise
Mitochondria
Skeletal Muscle
PGC1α
Mitochondrial Biogenesis
Intracellular Signalling
Lead sponsor: Queen's University
Sponsor class: OTHER
First posted: 2018-10-24
Enrollment: 13
Sex: MALE
Minimum age (years): 18
Maximum age (years): 30
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: CROSSOVER
Primary purpose: BASIC_SCIENCE

Brief summary: This crossover study investigates the effects of restricting arterial blood flow during cycling exercise on PGC-1α and its upstream signalling pathways. In a randomized order, participants will exercise once for 30 minutes with restricted blood flow and then will exercise once for 30 minutes with no blood flow restriction.


Eligibility criteria: Inclusion Criteria:

* 3 hours of physical activity per week
* No previous cycling training
* No concurrent involvement in another training program
* Body mass index \< 30 kg/m2

Exclusion Criteria:

* Presence of cardiovascular disease
* Presence of metabolic disease
* Taking regular oral medication
* Current smoker

Primary outcomes:
measure: Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) messenger ribonucleic acid (mRNA)
timeFrame: 15 minutes before exercise and 3 hours post-exercise for both exercise sessions
Secondary outcomes:
measure: Catecholamine concentrations
timeFrame: 15 minutes before exercise and immediately post-exercise for both exercise sessions
measure: Total and phosphorylated p38 mitogen-activated protein kinase (MAPK)
timeFrame: 15 minutes before exercise and immediately post-exercise for both exercise sessions
measure: Total and phosphorylated acetyl coenzyme A carboxylase (ACC)
timeFrame: 15 minutes before exercise and immediately post-exercise for both exercise sessions
measure: Total and phosphorylated protein kinase A (PKA)
timeFrame: 15 minutes before exercise and immediately post-exercise for both exercise sessions
measure: Acetylated p53
timeFrame: 15 minutes before exercise and immediately post-exercise for both exercise sessions
measure: Blood lactate
timeFrame: During both exercise sessions (lactate); 15 minutes before and immediately after both exercise sessions
measure: Heart rate (HR)
timeFrame: During both exercise sessions
measure: Revolutions per minute (RPM)
timeFrame: During both exercise sessions
measure: Ratings of perceived exertion (RPE)
timeFrame: During both exercise sessions
measure: Oxygenated and deoxygenated hemoglobin
timeFrame: During both exercise sessions and up to 10 minutes after (for cuff occlusion)
Other outcomes:
Locations:
city: Kingston
country: Canada
facility: Queen's University
state: Ontario
Study officials:
affiliation: Queen's University
name: Brendon J Gurd, PhD
role: PRINCIPAL_INVESTIGATOR
References: