Clinical trial NCT04057274: Acute Effect of modeRate-intensity aerOBIc Exercise on Colon Cancer Cell Growth
Official title: Effect of a Single Bout of Moderate-intensity Aerobic Exercise on Colon Cancer Cell Growth in Vitro
Study type: INTERVENTIONAL
Overall status: UNKNOWN
Phase: NA
Conditions: Colon Adenocarcinoma
Colon Cancer
Keywords: Colon cancer
Aerobic exercise
Lead sponsor: Northumbria University
Sponsor class: OTHER
Collaborators: name: York St John University
name: Newcastle University
First posted: 2019-08-15
Enrollment: 16
Sex: MALE
Minimum age (years): 50
Maximum age (years): 90
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: CROSSOVER
Intervention model description: This is a randomised, counterbalanced, crossover study whereby all participants will complete an exercise assessment and a resting assessment in a randomised order.
Primary purpose: PREVENTION

Brief summary: This study involves drawing blood samples from men before and after they perform 30-minutes of moderate-intensity aerobic exercise. The investigators will evaluate whether adding the exercise serum to colon cancer cells in a dish can reduce the growth of the cells compared to the resting serum.

Note: serum is the liquid part of the blood that carries hormones and metabolites around the body.

Detailed description: Regular exercise is associated with a reduced risk of developing colon cancer. However, the mechanisms underpinning the anti-cancer effect of exercise are not yet fully understood. A recent theory suggests that each time you exercise, the short-lasting spikes in circulating hormones can suppress the growth of cancer cells. Hence, every exercise bout could have a direct anti-cancer effect.

This study will recruit men with an increased of colon cancer and explore whether incubating colon cancer cells with serum collected after a bout of moderate-intensity aerobic exercise influences cell viability in vitro.

Eligibility criteria: Inclusion Criteria:

* Age ≥ 50 years
* BMI ≥ 25 kg/m2 and/or waist circumference of ≥ 94 cm
* Male
* Participating in less than 30 min of planned, structured, moderate to vigorous-intensity physical activity on three or more d·wk-1 for the last three months

Exclusion Criteria:

* Any absolute or relative contraindication to exercise testing, as determined by the American College of Sports Medicine
* Any sign/symptom of cardiovascular, metabolic or renal disease
* Known cardiovascular, metabolic or renal disease without written medical clearance from physician
* Resting hypertension (≥160 mmHg systolic and/or ≥90 mmHg diastolic)
* Chronic obstructive pulmonary disease and/or asthma with peak respiratory flow \< 300 l/min
* Previous stroke or transient ischemic attack
* Epilepsy or aneurysm (large vessel or cerebral)
* Previous or current treatment for malignancy
* Clotting disorder
* Taking beta-adrenergic blocking agents
* Resting heart rate ≥ 100 bpm
* Musculoskeletal, neurological, anthropometric, or rheumatoid conditions that makes it not possible to pedal a bicycle and/or would be worsened due to exercise
* Body mass \> 150 kg

Primary outcomes:
measure: Cell viability (%)
timeFrame: 48 hours
Secondary outcomes:
measure: Concentration of epinephrine (pg/ml)
timeFrame: Immediately before and immediately after the exercise and resting assessments
measure: Concentration of norepinephrine (pg/ml)
timeFrame: Immediately before and immediately after the exercise and resting assessments
measure: Concentration of Interleukin 6 (pg/ml)
timeFrame: Immediately before and immediately after the exercise and resting assessments
measure: Concentration of Tumour Necrosis Factor alpha (pg/ml)
timeFrame: Immediately before and immediately after the exercise and resting assessments
measure: Concentration of Insulin (pmol/l)
timeFrame: Immediately before and immediately after the exercise and resting assessments
measure: Concentration of Insulin-like growth factor 1 (ng/ml)
timeFrame: Immediately before and immediately after the exercise and resting assessments
Other outcomes:
Locations:
city: York
country: United Kingdom
facility: York St John University Sports Park
state: North Yorkshire
city: Newcastle Upon Tyne
country: United Kingdom
facility: Northumbria University City Campus
Study officials:
affiliation: Northumbria University
name: Samuel T Orange, PhD
role: PRINCIPAL_INVESTIGATOR
References:
citation: Boyle T, Keegel T, Bull F, Heyworth J, Fritschi L. Physical activity and risks of proximal and distal colon cancers: a systematic review and meta-analysis. J Natl Cancer Inst. 2012 Oct 17;104(20):1548-61. doi: 10.1093/jnci/djs354. Epub 2012 Aug 22.
citation: Dethlefsen C, Hansen LS, Lillelund C, Andersen C, Gehl J, Christensen JF, Pedersen BK, Hojman P. Exercise-Induced Catecholamines Activate the Hippo Tumor Suppressor Pathway to Reduce Risks of Breast Cancer Development. Cancer Res. 2017 Sep 15;77(18):4894-4904. doi: 10.1158/0008-5472.CAN-16-3125.
citation: Dethlefsen C, Pedersen KS, Hojman P. Every exercise bout matters: linking systemic exercise responses to breast cancer control. Breast Cancer Res Treat. 2017 Apr;162(3):399-408. doi: 10.1007/s10549-017-4129-4. Epub 2017 Jan 30.
citation: Devin JL, Hill MM, Mourtzakis M, Quadrilatero J, Jenkins DG, Skinner TL. Acute high intensity interval exercise reduces colon cancer cell growth. J Physiol. 2019 Apr;597(8):2177-2184. doi: 10.1113/JP277648. Epub 2019 Mar 20.