Clinical trial NCT02519257: The Relationship of Adiponectin in Adipose Tissue, Thy-1 in Plaques, and Inflammatory Mediators With Cardiac Diseases
Official title: The Relationship of Adiponectin in Adipose Tissue, Thy-1 in Plaques, and Inflammatory
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Heart Surgery
Keywords: CAD
VHD
Thy-1
Lead sponsor: Far Eastern Memorial Hospital
Sponsor class: OTHER
First posted: 2015-08-10
Enrollment: 96
Sex: ALL
Standard ages: CHILD
ADULT
OLDER_ADULT
Accepts healthy volunteers: no
Allocation: NON_RANDOMIZED
Intervention model: PARALLEL
Primary purpose: BASIC_SCIENCE

Brief summary: Atherosclerosis is an inflammatory process in which immune mechanisms interact with metabolic risk factors to initiate, propagate, and activate lesions in the arterial trees and is known as the main cause of coronary artery disease (CAD). Recently, there are more and more studies highlighted the potential importance of adipose tissue in relation to inflammation effects on CAD pathogenesis. However, it remains unclear whether Thy-1, adiponectin or any other inflammatory mediators in mediastinal adipose tissue contribute to CAD. In this study, we aim to analyze the expression of inflammatory mediators' expression via in vitro assay (3T3-L1 cell culture) and in vivo assay (human adipose tissues).

Detailed description: In addition, we'd like to investigate the association of the investigators' findings with clinical atherosclerotic risks, medications (statins or antiplatelet), and blood sugar. Now, we've established cell assay and successfully transformed mature adipocyte from 3T3-L1 cells. Besides, there were nighty-six patients enrolled into this study. Flow cytometry of Thy-1 and MCP-1 concentration in cultured medium by ELISA were also analyzed.

Eligibility criteria: Inclusion Criteria:

* coronary artery bypass grafting surgery
* valvular surgery

Exclusion Criteria:

* liver disease (GPT 2 times greater than normal limits)
* chronic renal insufficiency (Creatinine \> 2.0 mg/dL)
* neoplastic diseases
* taking steroids
* congestive heart failure

Primary outcomes:
measure: CD91 (Thy-1)
timeFrame: up to 24 months
Secondary outcomes:
measure: CD45
timeFrame: up to 24 months
Other outcomes:
Locations:
city: New Taipei City
country: Taiwan
facility: Far Eastern Memorial Hospital
Study officials:
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