Clinical trial NCT02978560: Indocyanine Green Tissue Perfusion Monitoring
Official title: Indocyanine Green Tissue Perfusion Monitoring
Study type: OBSERVATIONAL
Overall status: COMPLETED
Conditions: Regional Blood Flow
Wound Heal
Lead sponsor: University of Maryland, Baltimore
Sponsor class: OTHER
Collaborators: name: Baltimore Medical & Surgical Associates PA
First posted: 2016-12-01
Enrollment: 15
Sex: ALL
Minimum age (years): 21
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: yes
Observational model: CASE_CONTROL
Time perspective: PROSPECTIVE
Sampling method: NON_PROBABILITY_SAMPLE
Study population: 60

Brief summary: The aim of this project is to demonstrate that fluorescence-mediated photoplethysmography (FM-PPG) is capable of routinely acquiring the tissue perfusion data sufficient to detect and monitor skin tissue perfusion anomalies.

Detailed description: Methods to reliably predict which cutaneous wounds will heal are unreliable and clinical judgment inadequate. This is a problem for all health care providers and a common dilemma when evaluating patients in the Emergency Department. While capillary blood perfusion of the skin is a recognized requirement for healing, objective methods to reliably quantify this necessary physiological parameter are lacking. In large part, this is due to difficulties visualizing and recording capillary blood flow. Despite technical advances in blood flow imaging the capillary circulation remains poorly characterized. That is, current methodology can only image medium-to-large caliber vessels, not the small capillaries. The investigators hypothesize that with new technology, objective assessment of capillary flow can be achieved with fluorescence-mediated photoplethysmography (FM-PPG) methodology, and this evaluation can be used to objectively determine which wounds will heal promptly versus require adjunctive interventions.

FM-PPG is currently being used clinically in clinics worldwide as a way to image blood flow, but the apparatus provides only a relative measurement. Thus, this investigation is Observational in nature and not Interventional. With repeated evaluations over weeks, current FM-PPG allows an assessment to be made on whether wound conditions are improving. The investigators have a device containing new imaging software which, in theory, can offer precise quantification of capillary blood flow. If true, a single measurement may provide the data necessary to predict which patients are in danger of not healing and thus requiring aggressive, and at times expensive or painful interventions and - even worse - limb amputations. This protocol is to test proof-of-concept by first evaluating cutaneous capillary follow in 30 normal, healthy individuals and then 30 individuals presenting for treatment with a variety of skin wounds to a clinic.

FM-PPG methodology requires intravenous injection of a small amount of a fluorescent dye, indocyanine green (ICG). ICG is an FDA-approved agent that has been safely used in over 4000 patients. The area of skin to be measured is positioned below a camera and low-power laser instrument to take fluorescent pictures. The ICG is dissolved in sterile salt water for injection and about one and one half tablespoons injected into a vein. Typically, the measuring sequence consists of obtaining fluorescence images at the rate of approximately 30/second during a period of about 15 seconds. Following acquisition of the initial transit angiogram sequence, several additional 15-second-long image sequences are then acquired during the next half-hour, but without any additional injections. At about 60 seconds following dye injection, about two teaspoons of venous blood is withdrawn. This blood is used in the laboratory to determine the ICG dye concentration as a verification of the concentration determined by the camera images as part of the FM-PPG software algorithm (circulating dye concentration is needed in calculation of quantitative blood perfusion).

Since this study will be the first to employ a methodology capable of acquiring absolute tissue blood perfusion data from multiple subjects, there exists no database to indicate the normal range of variation in skin tissue perfusion that occurs in normal subjects. Since abnormal perfusion cannot be clearly recognized until the normal range of variation has been established, the first 30 subjects will be normal individuals. Then the following 30 subjects will be patients undergoing wound healing therapy.

Eligibility criteria: Inclusion Criteria:

1. Male or female subjects, of any ethnic background, who are at least 21 years of age and have normal healthy skin (30 normal subjects) or patients with diabetic foot ulcer lesions (30 patients).
2. All subjects must be willing and be able to comply with the protocol, and to provide informed consent.

Exclusion Criteria:

1. Patient currently participating in any other drug or device investigational study.
2. Patient with significant liver or kidney disease.
3. Patient with known adverse reaction to indocyanine green or iodine.
4. Patient is pregnant or nursing.
5. Patient with peripheral vascular disease and an ankle-brachial index blood flow of 0.6 or less

Primary outcomes:
measure: Indocyanine Green Tissue Perfusion Monitoring
timeFrame: 2 years
Secondary outcomes:
Other outcomes:
Locations:
city: Towson
country: United States
facility: Baltimore Medical & Surgical Associates PA
state: Maryland
Study officials:
affiliation: University of Maryland, Baltimore
name: Stephen R Thom, MD, PhD
role: PRINCIPAL_INVESTIGATOR
References:
citation: Flower RW, Mirza Z, Thom SR. Absolute quantification (ml blood/sec * mm2 tissue) of normal vs. diabetic foot skin microvascular blood perfusion: Feasibility of FM-PPG measurements under clinical conditions. Microvasc Res. 2019 May;123:58-61. doi: 10.1016/j.mvr.2018.12.004. Epub 2018 Dec 24.