Clinical trial NCT05760066: Effects of Resistance Training Preconditioning on Skeletal Muscle Recovery From a Period of Disuse in Young Adults
Official title: Effects of Resistance Training Preconditioning on Skeletal Muscle Recovery From a Period of Disuse in Young Adults
Study type: INTERVENTIONAL
Overall status: UNKNOWN
Phase: NA
Conditions: Atrophy, Disuse
Atrophy, Muscular
Lead sponsor: Auburn University
Sponsor class: OTHER
First posted: 2023-03-08
Enrollment: 24
Sex: ALL
Minimum age (years): 18
Maximum age (years): 30
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: PARALLEL
Primary purpose: TREATMENT

Brief summary: The goal of this clinical trial is to compare the effects of resistance training (RT) preconditioning vs no training on disuse-induced atrophy and post-disuse resistance training in young healthy individuals. The main questions it aims to answer are:

* To determine if performing RT prior to a period of disuse enhances the regain of strength, skeletal muscle size, and skeletal muscle quality while performing RT after a period of disuse.
* To determine if performing RT prior to a period of disuse dampens the maladaptive effects of disuse on muscle size, muscle quality, and strength.
* To determine the anabolic and proteolytic mechanisms underpinning the observed outcomes.

Participants will:

1. Perform either 6 weeks of resistance training or maintain an untrained lifestyle
2. Perform 2 weeks of limb immobilization induced disuse of a randomized leg
3. Perform 6 weeks of resistance training

Researchers will compare the resistance training preconditioning condition vs the non-trained condition to see if resistance training prior to a period of disuse is beneficial during the disuse period and in the return to training period on skeletal muscle size, strength, and underpinning molecular markers.


Eligibility criteria: Inclusion Criteria:

* Between the ages of 18-30
* Free from medical implants in lower extremity that would interfere with low-dose radiation
* Are not allergic to ultrasound gel or Lidocaine
* Have no or minimal experience with resistance training (less than 1 training session/week for a 1 year period)

Exclusion Criteria:

* Have been exposed to medically necessary radiation in the past 12 months other than dental x-rays
* Have known overt cardiovascular or metabolic disease
* Have a medical condition that would contraindicate participating in an exercise program or donating a skeletal muscle biopsy (e.g. blood clotting disorder, taking blood thinners, etc.)
* Are undergoing medical treatment for a disease that would interfere physiologically or logistically with study outcomes and/or protocols (e.g. undergoing chemotherapy and/or radiation for cancer treatment)
* Have consumed supplemental protein, creatine, and/or agents that affect hormone status (e.g. testosterone, growth hormone boosters, etc.) within the previous 2 months.

Primary outcomes:
measure: Change in vastus lateralis fiber cross-sectional area
timeFrame: Through completion of study, 16 weeks
measure: Change in quadriceps strength via 3 repetition maximum testing
timeFrame: Through completion of study, 16 weeks
measure: Change in quadriceps strength via isokinetic dynamometry
timeFrame: Through completion of study, 16 weeks
Secondary outcomes:
measure: Change in protein expression of anabolic signaling proteins
timeFrame: Through completion of study, 16 weeks
measure: Change in protein expression of catabolic signaling proteins
timeFrame: Through completion of study, 16 weeks
measure: Change in activity of catabolic systems
timeFrame: Through completion of study, 16 weeks
measure: Gene expression at the mRNA level of atrophy-associated genes (atrogenes)
timeFrame: Through completion of study, 16 weeks
Other outcomes:
Locations:
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