Clinical trial NCT05185167: Comparison of the Genotoxicity of Desflurane and Propofol
Official title: Genotoxic Effects of Desflurane and Propofol Anesthesia in Patients Undergoing Lumbar Disc Surgery
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: DNA Damage
Anesthesia
Keywords: Desflurane
Intravenous anesthetics
propofol
comet essay
Lead sponsor: Muğla Sıtkı Koçman University
Sponsor class: OTHER
First posted: 2022-01-11
Enrollment: 30
Sex: ALL
Minimum age (years): 18
Maximum age (years): 65
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: SEQUENTIAL
Primary purpose: DIAGNOSTIC

Brief summary: The purpose of this study is to evaluate if the anesthetics propofol and desflurane can damage DNA according to comet essay in patients submitted to lumbar disc surgery.

Detailed description: It is expected that DNA damage information provide the possible genotoxic effects of inhalational and intravenous anesthetics and contribute to the establishment of strategies aimed at reducing the risks to exposed patients.

Eligibility criteria: Inclusion Criteria:

1. Ability to understand and willingness to sign an informed consent form
2. Elective lumbar spinal surgery that will take at least 120 minutes,
3. American Society of Anesthesiologists (ASA) physical status I and II

Exclusion Criteria:

1. Patients with ASA physical status III or IV disease
2. Chronic systemic disease (diabetes mellitus,chronic pulmonary disorders, chronic kidney and liver disorders)
3. Smoking or alcohol consumption
4. Recently received radiation, chemotherapy
5. Malignancy
6. Patients who will receive blood transfusion during the operation
7. Users of antioxidant supplements
8. Patients who have received general anesthesia in the last three months
9. Patients with known occupational exposures (operating room personnel, chemical plant worker

Primary outcomes:
measure: Intensity of the DNA tail detected by using Comet Assay IV Version 4.3.2 for Basler FireWire
timeFrame: Before anesthesia induction
measure: Intensity of the DNA tail detected by using Comet Assay IV Version 4.3.2 for Basler FireWire
timeFrame: 2 hours after anesthesia induction
measure: Intensity of the DNA tail detected by using Comet Assay IV Version 4.3.2 for Basler FireWire
timeFrame: postoperative 1st day
measure: Intensity of the DNA tail detected by using Comet Assay IV Version 4.3.2 for Basler FireWire
timeFrame: postoperative 5th day
measure: Head length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire
timeFrame: Before anesthesia induction
measure: Head length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire
timeFrame: 2 hours after anesthesia induction
measure: Head length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire
timeFrame: postoperative 1 st day
measure: Head length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire
timeFrame: postoperative 5th day
measure: Tail length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire
timeFrame: before anesthesia induction
measure: Tail length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire
timeFrame: 2 hours after anesthesia induction
measure: Tail length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire
timeFrame: postoperative 1 st day
measure: Tail length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire
timeFrame: postoperative 5th day
Secondary outcomes:
Other outcomes:
Locations:
city: Mugla
country: Turkey
facility: Mugla Sitki Kocman University
Study officials:
affiliation: Mugla Sıtkı Kocman University Department of Anesthesiology and reanimation
name: Melike Korkmaz Toker, Assoc. Prof.
role: PRINCIPAL_INVESTIGATOR
References: