Clinical trial NCT05947526: Outcomes of the Embryos of Different Ovarian Follicle Sizes, and the miRNA Detection in Their Blastocyst Culture
Official title: Evaluation of the Outcomes of the Embryos Obtained From Different Follicle Sizes, and the miRNA Detection in the Spent Media of Their Blastocyst Culture
Study type: OBSERVATIONAL
Overall status: RECRUITING
Conditions: Embryo Quality
Keywords: follicle size
micro RNA
blastocyst culture
embryo quality
Lead sponsor: Acibadem University
Sponsor class: OTHER
First posted: 2023-07-17
Enrollment: 55
Sex: FEMALE
Minimum age (years): 20
Maximum age (years): 38
Standard ages: ADULT
Accepts healthy volunteers: yes
Observational model: COHORT
Time perspective: PROSPECTIVE
Sampling method: NON_PROBABILITY_SAMPLE
Study population: Patients who are going to be included to the study will be the patients of infertility clinic

Brief summary: In this study, it is aimed to analyze the outcomes of embryos obtained from ovarian follicle with different sizes, and identify the microRNA expression in the spent media of the blastocysts cultures.

Detailed description: In this study, it is aimed to analyze the outcomes of embryos obtained from ovarian follicle with different sizes. By this way, we assume to predict which follicle is able to give the best blastocyst. After the blastocysts are obtained from the different sized follicles, blastocyst cultures are going to be studied for the up and down-regulations of microRNA, besides the correlation of embryo quality with miRNA expression in the spent media.

Eligibility criteria: Inclusion Criteria:

* Female patients aged between 20-38 who are planned to have IVF treatment
* On the day of trigger before the oocyte pick-up, patients having at least 5, and 14 at most follicles exceeding 11mm
* Patient having BMI below 30

Exclusion Criteria:

* PCO patients
* Patients having endometriosis
* Patients having male factor with severe oligoazoospermia

Primary outcomes:
measure: Oocyte grading
timeFrame: after each hyper stimulation process of the patients, when the follicle sizes are large enough to pick up which is roughly 10-15 days after the hyper stimulation has started
measure: Day-3 Embryo Grading
timeFrame: Three days after the intracytoplasminc sperm injection(ICSI) procedure
measure: Day-5 Embryo Grading
timeFrame: 5 days after the intracytoplasminc sperm injection(ICSI) procedure
measure: beta hCG results of the transferred embryos
timeFrame: ten days after the embryo transfer
Secondary outcomes:
measure: micro RNA expression in culture medium
timeFrame: Culture mediums of day 5 embryos will be collected to be studied, after the last embryo is obtained and all will be studied at the end of the recruitment
Other outcomes:
Locations:
city: Istanbul
country: Turkey
facility: Acibadem University Atakent Hospital
Study officials:
affiliation: Acıbadem University School of Medicine
name: NAZLI ALBAYRAK, MD
role: PRINCIPAL_INVESTIGATOR
References:
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