Clinical trial NCT02115594: Phase 2 Study of Fulvestrant With and Without Entinostat in Postmenopausal Women With ER+ Advanced Breast Cancer
Official title: A Phase 2, Randomized, Double-Blind, Multicenter Study of Fulvestrant With and Without Entinostat in Postmenopausal Women With Hormone Receptor-Positive Advanced Breast Cancer
Study type: INTERVENTIONAL
Overall status: WITHDRAWN
Phase: PHASE2
Conditions: Breast Cancer
Keywords: metastatic breast cancer
estrogen receptor
Histone Deacetylase Inhibitors
Histone Deacetylases
HDAC inhibitors
selective estrogen receptor modulator
estrogen receptor modulator, selective
Estrogen receptor modulators, selective
Lead sponsor: Syndax Pharmaceuticals
Sponsor class: INDUSTRY
First posted: 2014-04-16
Enrollment: 0
Sex: FEMALE
Standard ages: CHILD
ADULT
OLDER_ADULT
Accepts healthy volunteers: no
Allocation: RANDOMIZED
Intervention model: PARALLEL
Primary purpose: TREATMENT

Brief summary: Preclinical data has demonstrated that entinostat (SNDX-275) can enhance fulvestrant sensitivity in hormone receptor-positive breast cancer in animal models. The addition of entinostat to fulvestrant will provide clinical benefit to patients with locally advanced or metastatic breast when compared to fulvestrant plus placebo. Also, based on previous data, patients exposed to entinostat who demonstrate an elevated level of protein lysine acetylation will have an improved efficacy outcome.

Detailed description: Entinostat (SNDX-275) inhibits mechanisms of resistance to hormone therapy in breast cancer (BC) cells, thereby prolonging sensitivity of the cells to fulvestrant. Preclinical data has demonstrated that entinostat can enhance fulvestrant sensitivity in hormone receptor-positive BC in animal models. Thus, it is hypothesized that the addition of entinostat to fulvestrant will provide clinical benefit to patients with locally advanced or metastatic BC when compared to fulvestrant plus placebo.

Preliminary data from Phase 2 Study SNDX-275-0301 suggest patients with higher levels of protein lysine acetylation who receive entinostat with exemestane potentially have improved clinical outcomes (e.g., PFS, OS) when compared to patients with lower levels of protein lysine acetylation. Thus, it is hypothesized that patients exposed to entinostat and who demonstrate an elevated level of protein lysine acetylation will have an improved efficacy outcome.

Eligibility criteria: Inclusion Criteria:

* Patient is a female who is: Postmenopausal OR Pre/perimenopausal and: Received at least one prior hormone therapy in combination with a luteinizing hormone-releasing hormone (LHRH) agonist prior to study entry. Initiated on an LHRH agonist at least 28 days prior to study entry. Demonstrated ovarian estradiol suppression, defined as an estradiol level within postmenopausal ranges per institutional guidelines, within 28 days immediately prior to study entry
* Patient has histologically or cytologically confirmed ER+ and/or progesterone receptor-positive (PR+) BC at initial diagnosis or on subsequent biopsy. With regard to hormone receptor status, staining of ≥1% cells is considered positive. Receptor status may have been determined at any time prior to randomization and from any site (i.e., primary, recurrent, or metastatic)
* Patient experienced PD within 28 days before initiating study treatment and has been deemed eligible for treatment with fulvestrant
* Patient has evidence of locally advanced or metastatic disease, based on imaging studies (bone scan, CT, MRI) within 28 days before initiating study treatment
* Patient completed any prior radiotherapy ≥2 weeks prior to receiving the first dose of study treatment and has recovered from any radiation-related toxicity
* Patient may have received 1 prior chemotherapy regimen for metastatic disease provided treatment was completed ≥3 weeks prior to randomization. Prior chemotherapy in the adjuvant or neoadjuvant setting is also allowed
* Patient is willing and able to provide or assist study personnel in accessing slides from prior biopsies
* Patient has an Eastern Cooperative Oncology Group (ECOG) performance status of 0 or 1
* Patient has the following laboratory parameters: a. Hemoglobin (Hgb) ≥9.0 g/dL; unsupported platelet count ≥100×10P9P/L; and absolute neutrophil count (ANC) ≥1.5×10P9P/L without the use of hematopoietic growth factors; b. Creatinine ≤2.0 mg/dL; c. Total bilirubin \<1.5 x institutional upper limit normal (≤3 mg/dL in case of Gilbert's syndrome); d. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) ≤2 x institutional upper limit of normal; unless elevation is due to metastatic disease to the liver, in which case ALT and AST must be within 5.0 x institutional upper limit of normal
* Patient is able to swallow tablets
* Patient is able to understand and give written informed consent and comply with study procedures

Exclusion Criteria:

* Patient has rapidly progressive or life-threatening metastases (visceral crisis)
* Patient has HER2-positive (HER2+) disease, as defined by the 2013 American Society of Clinical Oncology (ASCO)/College of American Pathologists (CAP) recommendations for HER2 testing in BC (see http://www.asco.org/quality-guidelines/recommendations-human-epidermal-growth-factor-receptor-2-testing-breast-cancer), or has unknown HER2 status
* Patient previously received treatment with entinostat or any other HDAC inhibitor, including valproic acid
* Patient has had previous treatment with fulvestrant or other selective estrogen receptor down-regulator (SERD) in the metastatic setting; such treatment may have been given in the adjuvant setting
* Patient has an allergy to benzamide or inactive components of the study drug
* Patient has a history of allergies to any active or inactive ingredients of fulvestrant
* Patient has a concomitant medical condition that precludes adequate study treatment compliance or assessment, or increases patient risk, in the opinion of the Investigator, such as but not limited to:

  1. Myocardial infarction or arterial thromboembolic event within 6 months prior to Baseline or severe or unstable angina, New York Heart Association (NYHA) Class III or IV disease (see Appendix 2), or a QTc interval \>470 msec.
  2. Uncontrolled heart failure or hypertension, uncontrolled diabetes mellitus, or uncontrolled systemic infection
  3. Another active cancer (excluding adequately treated basal cell carcinoma or cervical intraepithelial neoplasia \[CIN\] / cervical carcinoma in situ or melanoma in situ). Prior history of other cancer is allowed, as long as there is no active disease within the prior 5 years
* Patient is currently receiving treatment with any agent listed on the prohibited medication list such as valproic acid or other systemic cancer agents
* Patient initiated oral bisphosphonates within 7 days prior to study drug
* Patient has moderate or severe hepatic impairment (i.e., Child-Pugh score B or C)
* Patient has brain or leptomeningeal metastases

Primary outcomes:
measure: Progression Free Survival
timeFrame: From date of randomization until the date of 1st documented progression or date of death from any cause, whichever occurs first, assessed for up to 48 months
Secondary outcomes:
measure: Objective Response Rate (CR or PR)
timeFrame: From date of randomization until the date of 1st documented progression or date of death from any cause, whichever occurs first, for up to 48 months
measure: Clinical Benefit Rate (CR, PR, or SD for greater than or equal to 6 months from randomization)
timeFrame: From the date of randomization until the date of 1st documented progression or date of death, from any cause, whichever occurs first, assessed for up to 48 months
measure: Overall Survival
timeFrame: From the date of randomization until date of death, assessed for up to 48 months
measure: Clinical review of safety parameters (AEs, lab values)
timeFrame: From date of randomization until 30 days post the date of study treatment discontinuation
measure: Percent change from baseline in blood protein lysine acetylation measures
timeFrame: From the baseline visit through the 1st 15 days of study treatment
measure: Concentrations of entinostat measured in plasma (PK)
timeFrame: From the baseline visit through the 1st 15 days of study treatment
Other outcomes:
measure: Analysis of biopsy tumor tissue (fresh optional, archival required)
timeFrame: Screening (fresh tissue) and post 1st dose, between Days 15-18 of study treatment
Locations:
city: Nashville
country: United States
facility: Tennessee Oncololgy
state: Tennessee
Study officials:
affiliation: Tennessee Oncology / Sarah Cannon
name: Denise Yardley, MD
role: PRINCIPAL_INVESTIGATOR
References: