Clinical trial NCT03625791: Validation of a Genetic Signature to Predict the Development of Sarcomas
Official title: Validation of a Genetic Signature to Predict the Development of Sarcomas
Study type: OBSERVATIONAL
Overall status: COMPLETED
Conditions: Genetic Signature in Development of Sarcomas
Keywords: sarcoma
genetic
Lead sponsor: Centre Georges Francois Leclerc
Sponsor class: OTHER
First posted: 2018-08-10
Enrollment: 60
Sex: ALL
Minimum age (years): 18
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: no
Observational model: COHORT
Time perspective: PROSPECTIVE
Sampling method: PROBABILITY_SAMPLE
Study population: * Group 1: 20 patients with sarcoma developed in irradiated territory
* Group 2: 20 patients treated for cancer by radiotherapy for more than 5 years and who have not developed sarcoma
* Group 3: 20 patients with primary sarcoma

Brief summary: The aim of the SARI study was to describe biomarkers of predisposition to the development of sarcomas in irradiated territory. This study included 120 patients with sarcoma in irradiated territory and 240 patients who had been treated with radiotherapy for more than 5 years and had not developed sarcoma.

Following the sequencing of the exomes of all these patients, the SARI study made it possible to highlight a genetic signature from 11 genes, predictive of the appearance of a sarcoma after a first radiotherapy. This signature is the subject of a patent (BFF 170286 / VF, filed on June 22, 2017). A final validation step with samples that have not been used to optimize this signature is now required. Moreover, it is now necessary to validate if this signature is specific to the predisposition to the development of radiation-induced sarcomas only or if this signature is also valid for the predisposition to the development of all sarcomas, even primary ones.

The objective of the PREDISARC study is to evaluate the specificity of this genetic signature (11 genes) with the appearance of sarcomas in irradiated territory compared to a population without sarcoma that has been treated with radiotherapy.

Detailed description: A total of 60 patients will be included in this pilot study:

* Group 1: 20 patients with induced radio sarcoma
* Group 2: 20 patients without primary sarcoma or induced radio sarcoma
* Group 3: 20 patients with primary sarcoma These numbers will make it possible to have preliminary data making it possible to judge the interest of the genetic signature in the prediction of the radiation-induced sarcomas.

Eligibility criteria: Inclusion Criteria:

1. Major patient at the time of initial radiotherapy or diagnosis of primary sarcoma.
2. Patient belonging to one of these 3 groups :

   Group 1: Patient treated with radiotherapy for cancer other than primary sarcoma and who developed sarcoma (other than osteosarcoma) in the irradiated area.

   OR Group 2: Patient who received radiotherapy for more than 5 years and did not develop sarcoma or other secondary cancers OR Group 3: Patient who developed primary sarcoma and who had never been treated with radiotherapy
3. Patient who have signed informed consent to participate in the study.
4. Affiliation to the social security system.

Exclusion Criteria:

1. Patient unable to understand, read and / or sign informed consent.
2. Person benefiting from a protection system for adults (including guardianship and trusteeship)
3. Pregnant or lactating woman.
4. Patient who participated in the SARI study "Predictive study of sarcomas developed in irradiated territory from the GSF-GETO database (ID-RCB no .: 2011-A01102-39)"

Primary outcomes:
measure: DNA
timeFrame: at the inclusion
Secondary outcomes:
Other outcomes:
Locations:
city: Bordeaux
country: France
facility: Institu Bergonie
city: Dijon
country: France
facility: Centre Georges François Leclerc
city: Villejuif
country: France
facility: Institut Gustave Roussy
Study officials:
affiliation: CGFL, Dijon
name: Gilles TRUC
role: PRINCIPAL_INVESTIGATOR
References: