Clinical trial NCT02637167: GutHeart: Targeting Gut Microbiota to Treat Heart Failure
Official title: GutHeart: Targeting Gut Microbiota to Treat Heart Failure
Study type: INTERVENTIONAL
Overall status: UNKNOWN
Phase: PHASE2
Conditions: Systolic Heart Failure
Keywords: Heart failure
inflammation
rifaximin
Saccharomyces boulardii
gut microbiota
Lead sponsor: Oslo University Hospital
Sponsor class: OTHER
First posted: 2015-12-22
Enrollment: 150
Sex: ALL
Minimum age (years): 18
Maximum age (years): 74
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: no
Allocation: RANDOMIZED
Intervention model: PARALLEL
Primary purpose: TREATMENT

Brief summary: The objective of this trial is to study the effect of targeting the gut microbiota in patients with heart failure (HF). First, the investigators will characterize gut microbiota composition in patients with various degree of systolic HF as compared with healthy controls. Second, the potential impact of targeting gut microbiota to improve HF will be investigated through an open label randomized controlled trial (RCT) of probiotics, antibiotics and controls. The hypothesis being tested is that the gut microbiota is altered in HF; that gut microbiota of HF patients, through interaction with the intestinal and systemic innate immune system, contribute to a low-grade systemic inflammation as well as metabolic disturbances in these patients; and that an intervention with probiotics and the non-absorbable antibiotic Rifaximin attenuates these inflammatory and metabolic disturbances and improves heart function through modulation of the gut microbiota.

Detailed description: While most studies on inflammation in heart failure (HF) have focused on down-stream mediators of inflammation and tissue damage, the present study will focus on alterations of the gut microbiota as a potential upstream arm in the activation of inflammatory responses. The gut microbiota may play a central role not only in the inflammatory arm of the pathogenesis of HF, but could also be involved in the induction of metabolic disturbances that contribute to the progression of this disorder. Decompensated HF is characterized by decreased cardiac output and congestion, contributing to edema and ischemia of the gut wall. Consequently, structural and functional changes occur, causing increased gut permeability.

Several studies have shown that low grade leakage of microbial products such as lipopolysaccharides (LPS), occurs across the gut wall, potentially causing systemic inflammation by activation of Toll like receptors (TLRs). Very small amounts of LPS have been shown to effectively induce release of TNFα 6, which acts as a cardiosuppressor via several pathways, including reduced mitochondrial activity, altered calcium homeostasis and impaired β-adrenergic signaling in cardiomyocytes. Furthermore, the investigators have recently shown that the microbiota-dependent marker TMAO is associated with clinical outcome in chronic HF. Interestingly, gut decontamination with antibiotics have been shown to reduce intestinal LPS-levels, monocyte expression of the LPS-receptor CD14 and production of TNFα. In addition, selective gut decontamination has improved postoperative outcome in cardiac surgery patients. However, at present there are no studies that have fully characterized the gut microbiota in HF patients and our knowledge of the interaction between gut microbiota, systemic inflammatory, metabolic disturbances and myocardial dysfunction in these patients are scarce.

This project will focus on the gut microbiota as a potential therapeutic target in HF, through an open label randomized controlled trial (RCT) of probiotics, antibiotics and controls, with improved heart function as primary end point.

Eligibility criteria: Inclusion Criteria:

* Must be at least 18 years of age, and less than 75.
* Have heart failure in New York Heart Association class II or III
* Echocardiographically verified LVEF \< 40 %.
* On optimal treatment for at least 3 months
* Must have lab values as the following:

Hemoglobin above 10 g/l; eGFR above 30 ml/min; ALT \< 150 units/l

* Signed informed consent and expected cooperation of the patients for the treatment and follow up must be obtained and documented according to ICH GCP, and national/local regulations.

Exclusion Criteria:

* Treatment with antibiotics or probiotics within the last 12 weeks
* History of hypersensitivity to Rifaximin or other Rifamycin derived antimicrobial agents, or any of the components of Xifaxan
* History of hypersensitivity to S. boulardii, yeast, or any of the components of Precosa
* Polypharmacia with increased risk for interactions. i.e. patient with an extensive medication lists (e.g. 10 drugs or more) which may influence with the patient safety or compromise the study results
* Malignancy of any cause, excluding basal cell carcinoma of the skin
* Acute coronary syndrome over the last 12 weeks
* Severely impaired kidney function (i.e., estimated glomerular filtration rate \< 30 ml/minute/1.73 m2)
* Impaired liver function (Alanine aminotransferase \> 150 U/l) or decompensated liver cirrhosis classified as Child-Pugh B or C.
* On-going infection, including GI infection
* Inflammatory bowel disease
* Bowel obstruction
* Active myocarditis, including Chagas disease
* Severe primary valvular heart disease
* Atrial fibrillation with ventricular frequency \> 100/min
* Any other, severe co morbid disease that must be expected to severely reduce the efficacy of the interventional products, survival or compliance
* Treatment with immunosuppressive drugs
* Treatment with rifamycins other than Rifaximin
* Central venous catheter
* Pregnancy or planned pregnancy
* Nursing
* Poor compliance
* Any reason why, in the opinion of the investigator, the patient should not participate

Primary outcomes:
measure: baseline-adjusted LVEF as measured by echocardiography
timeFrame: after 3 months of intervention
Secondary outcomes:
measure: Chao1 (index)
timeFrame: at baseline
measure: Chao1 (index)
timeFrame: after 3 months
measure: Chao1 (index)
timeFrame: after 6 months
measure: TMAO
timeFrame: at baseline
measure: TMAO
timeFrame: after 3 months
measure: Left ventricular end diastolic volume
timeFrame: at baseline
measure: Left ventricular end diastolic volume
timeFrame: after 3 months
measure: CRP
timeFrame: at baseline
measure: CRP
timeFrame: after 3 months
measure: Health-related quality of life score
timeFrame: at baseline and after 3 months
measure: Functional capacity
timeFrame: at baseline and after 3 months
measure: Number of patients with adverse events (any event)
timeFrame: at baseline, after 1 month, after 3 month and after 6 months
measure: Number of adverse events (any event)
timeFrame: at baseline, after 1 month, after 3 month and after 6 months
Other outcomes:
Locations:
city: Oslo
country: Norway
facility: Oslo University Hospital - Rikshospitalet
Study officials:
affiliation: Oslo University Hospital
name: Lars L Gullestad, MD, Prof
role: PRINCIPAL_INVESTIGATOR
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