Clinical trial NCT06053125: A Study of Adipose Tissue in Adaptive Responses to Exercise
Official title: The Role of Adipose Tissue in Adaptive Responses to Exercise
Study type: INTERVENTIONAL
Overall status: RECRUITING
Phase: NA
Conditions: Healthy
Obesity
Keywords: exercise
adipose
cytokines
Lead sponsor: Mayo Clinic
Sponsor class: OTHER
Collaborators: name: National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
First posted: 2023-09-25
Enrollment: 60
Sex: ALL
Minimum age (years): 18
Maximum age (years): 85
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: yes
Allocation: NON_RANDOMIZED
Intervention model: PARALLEL
Primary purpose: BASIC_SCIENCE

Brief summary: The purpose of this research is to determine how exercise affects fat (adipose) tissue and how changes to adipose tissue that occur during and after exercise might improve health in aging and obesity.

Detailed description: Adipose tissue has important endocrine functions that influence metabolic health. Early evidence shows that adipose adapts to physiological stress, including exercise. The objective here is to determine how exercise-induced alterations in adipose tissue cellular composition and endocrine signaling may contribute to the beneficial adaptations to exercise in aging and obesity.

Immune cell populations and inflammatory signatures will be assessed in subcutaneous abdominal adipose tissue biopsies collected from obese and normal weight young and older adults before, immediately after, and 3 hours after a 30-min bout of cycling exercise at 70% of maximal oxygen consumption (VO2max). Mass spectrometry, Olink targeted inflammation assays, and RNA sequencing will be used for full proteomic and transcriptomic characterization of the adipose tissue secretome (the proteins and molecules secreted from the adipose tissue) and the cargo of extracellular vesicles isolated from plasma and media collected from cultured human adipose tissue explants generated from each time point.

Overall, the primary hypothesis of the proposed work is that a single bout of exercise triggers transient changes in adipose tissue paracrine/endocrine signals and immune cellular composition. The investigators propose that these responses contribute to the beneficial effects of exercise locally and in distal tissues and that the cumulative effects of acute changes in adipose tissue likely contribute to the positive alterations in adipose tissue associated with exercise training.

Eligibility criteria: Inclusion Criteria:

* Adults between the ages of 18-35 OR 65-85.
* BMI 18.5 kg/m2 - 40 kg/m2
* Weight stable (≥ 3 months)
* Generally healthy

Exclusion Criteria:

* Participation in ≥ 30 minutes of structured physical activity ≥ 2 days per week.
* Smoking/tobacco use.
* Alcohol/substance abuse.
* Pregnancy and breastfeeding.
* Anemia.
* Abnormal renal function.
* Blood clotting disorders.
* Coronary artery disease.
* Uncontrolled thyroid disease.
* Liver disease.
* Use of medications known to influence the main outcomes of the study.
* Orthopedic problems that may be aggravated by exercise.
* Chronic disease at the discretion of the investigators.

Primary outcomes:
measure: Adipose Immune Cell Populations
timeFrame: Pre-exercise, immediately post-exercise, and 3 hours after exercise
measure: Inflammatory proteins secreted from adipose tissue measured using the Olink Target Inflammation Panel (includes 96 inflammation-associated proteins)
timeFrame: Pre-exercise, immediately post-exercise, and 3 hours after exercise
measure: Adipose tissue macrophage populations
timeFrame: Pre-exercise, immediately post-exercise, and 3 hours after exercise
measure: Adipose tissue gene expression of interleukin (IL)-6 measured by polymerase chain reaction (PCR).
timeFrame: Pre-exercise, immediately post-exercise, and 3 hours after exercise
measure: Adipose tissue gene expression of interleukin (IL)-8 measured by PCR.
timeFrame: Pre-exercise, immediately post-exercise, and 3 hours after exercise
measure: Adipose tissue gene expression of cluster of differentiation 68 (CD68) measured by PCR.
timeFrame: Pre-exercise, immediately post-exercise, and 3 hours after exercise
measure: Adipose tissue gene expression of cluster of differentiation 163 (CD163) measured by PCR.
timeFrame: Pre-exercise, immediately post-exercise, and 3 hours after exercise
measure: Adipose tissue gene expression of cluster of differentiation 206 (CD206) measured by PCR.
timeFrame: Pre-exercise, immediately post-exercise, and 3 hours after exercise
measure: Adipose tissue gene expression of adhesion G-protein coupled receptor E (ADGRE) measured by PCR.
timeFrame: Pre-exercise, immediately post-exercise, and 3 hours after exercise
measure: Adipose tissue gene expression of inducible nitric oxide synthase (INOS) measured by PCR.
timeFrame: Pre-exercise, immediately post-exercise, and 3 hours after exercise
measure: Adipose tissue gene expression of monocyte chemoattractant protein-1 (MCP1) measured by PCR.
timeFrame: Pre-exercise, immediately post-exercise, and 3 hours after exercise
measure: Adipose tissue gene expression of tumor necrosis factor alpha (TNFa) measured by PCR.
timeFrame: Pre-exercise, immediately post-exercise, and 3 hours after exercise
measure: Adipose tissue gene expression of leptin (LEPD) measured by PCR.
timeFrame: Pre-exercise, immediately post-exercise, and 3 hours after exercise
measure: Adipose tissue gene expression of adiponectin (ADIPOQ) measured by PCR.
timeFrame: Pre-exercise, immediately post-exercise, and 3 hours after exercise
measure: Inflammatory proteins found in adipose tissue measured using the Olink Target Inflammation Panel (includes 96 inflammation-associated proteins)
timeFrame: Pre-exercise, immediately post-exercise, and 3 hours after exercise
measure: Protein cargo of adipose extracellular vesicles measured using mass spectrometry
timeFrame: Pre-exercise, immediately post-exercise, and 3 hours after exercise
Secondary outcomes:
measure: Adipocyte cell size
timeFrame: Pre-exercise, immediately post-exercise, and 3 hours after exercise
measure: Plasma inflammatory cytokine concentrations measured using the Olink Target Inflammation Panel (includes 96 inflammation-associated proteins)
timeFrame: Pre-exercise, immediately post-exercise, and 3 hours after exercise
measure: Protein cargo of plasma extracellular vesicles measured using mass spectrometry extracellular vesicles
timeFrame: Pre-exercise, immediately post-exercise, and 3 hours after exercise
Other outcomes:
measure: Body fat percentage
timeFrame: Baseline
measure: Cardiorespiratory Fitness
timeFrame: Baseline
measure: White blood count
timeFrame: Baseline
measure: Fasting plasma insulin
timeFrame: Baseline
measure: Fasting plasma glucose
timeFrame: Baseline
measure: Hemoglobin A1c (HbA1c)
timeFrame: Baseline
Locations:
city: Rochester
country: United States
facility: Mayo Clinic
state: Minnesota
Study officials:
affiliation: Mayo Clinic
name: Hawley Kunz, PhD
role: PRINCIPAL_INVESTIGATOR
References: