Clinical trial NCT03951857: Effect of Aging & Obesity With Exercise Intervention
Official title: Biphasic and Combined Effect of Aging & Obesity With Development of Effective Exercise Intervention Protocol
Study type: INTERVENTIONAL
Overall status: COMPLETED
Phase: NA
Conditions: Obesity
Keywords: Aging
Obesity
Single muscle fiber contractile properties
Intramyocellular lipids
Resistance exercise
Lead sponsor: Seoul National University Bundang Hospital
Sponsor class: OTHER
Collaborators: name: National Research Foundation of Korea
First posted: 2019-05-15
Enrollment: 28
Sex: ALL
Minimum age (years): 20
Maximum age (years): 80
Standard ages: ADULT
OLDER_ADULT
Accepts healthy volunteers: yes
Allocation: NON_RANDOMIZED
Intervention model: PARALLEL
Primary purpose: SUPPORTIVE_CARE

Brief summary: Obesity leads to a vicious circle that increases intramuscular fat, insulin resistance, promotes muscular dysfunction resulting in increased muscle fat accumulation. The study of muscle function and intramyocellular lipids is insufficient for obesity. Particularly, the study of mechanisms in muscle function and intramyocellular lipids is few nationally and internationally.

Detailed description: This study is prospective study. The goal of this study is

1. To examine the distribution and contractile properties (cross-sectional area, maximal force, specific force, maximal shortening velocity) of single muscle fiber sex/Myosin heavy chain (MHC) type-related differences, by extracting a single muscle fiber from the vastus lateralis in young men and women.
2. To examine the effects of intramyocellular lipids on physical activity ability, muscle dysfunction, and metabolic disorders in relation to aging and obesity.
3. To examine the effects of intramyocellular lipids on physical activity ability, muscular dysfunction, and metabolic disorders after 12 weeks resistance exercise intervention.

Eligibility criteria: Inclusion Criteria:

* Young men: Age (20-35 yrs), BMI (19-23 Kg/m2)
* Young women: Age (20-35 yrs), BMI (19-23 Kg/m2)
* Elderly women: Age (65-80 yrs), BMI (19-23 Kg/m2)
* Elderly obese women: Age (65-80 yrs), BMI ( \>25 Kg/m2)
* Subjects who can agree voluntarily.

Exclusion Criteria:

* Acute coronary syndrome.
* Uncontrolled hypertension.
* Subjects who took drugs which can affects neuromuscular system.
* Severely impaired cognition
* Subjects who cannot agree voluntarily

Primary outcomes:
measure: Total droplet area
timeFrame: Before intervention / after 12weeks intervention
measure: Number of lipid droplets
timeFrame: Before intervention / after 12weeks intervention
measure: Single muscle fiber cross-sectional area (CSA)
timeFrame: Before intervention / after 12weeks intervention
measure: Single muscle fiber maximal force (Po)
timeFrame: Before intervention / after 12weeks intervention
measure: Single muscle fiber specific force (SF)
timeFrame: Before intervention / after 12weeks intervention
measure: Single muscle fiber maximal shortening velocity (Vo)
timeFrame: Before intervention / after 12weeks intervention
measure: Single muscle fiber MHC type distribution
timeFrame: Before intervention / after 12weeks intervention
Secondary outcomes:
measure: Hand grip strength
timeFrame: Before intervention / after 12weeks intervention
measure: Isokinetic knee extension strength
timeFrame: Before intervention / after 12weeks intervention
Other outcomes:
Locations:
city: SeongNam
country: Korea, Republic of
facility: Seoul National University Bundang Hospital
state: Gyeonggi
Study officials:
affiliation: Seoul National University Bundang Hospital
name: Jae-Youn Lim, Ph.D.
role: STUDY_DIRECTOR
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