Clinical trial NCT06054620: The Effect of Exercise and Presleep Nutrition on Muscle Connective Tissue Remodelling
Official title: The Effect of Exercise and Presleep Nutrition on Muscle Connective Tissue Remodelling
Study type: INTERVENTIONAL
Overall status: RECRUITING
Phase: NA
Conditions: Interventional
Keywords: Collagen
Vitamin C
Carbohydrate placebo
Lead sponsor: University of Toronto
Sponsor class: OTHER
First posted: 2023-09-26
Enrollment: 12
Sex: ALL
Minimum age (years): 18
Maximum age (years): 35
Standard ages: ADULT
Accepts healthy volunteers: yes
Allocation: RANDOMIZED
Intervention model: CROSSOVER
Intervention model description: Crossover to receive Collagen and Vitamin C (CVC) or Carbohydrate placebo. Unilateral leg exercise (EX) to give 4 outcomes. (1) CVC-EX; (2) CVC-REST; (3) PLACEBO-EX; (4) PLACEBO-REST
Primary purpose: BASIC_SCIENCE

Brief summary: The primary objective of this study will be to determine the influence of a presleep vitamin C-enriched, collagen-modelled supplement (CVC) on the metabolic fate of dietary glycine using a \[2H5\]glycine tracer within the intramuscular connective tissue at rest and after a bout of resistance exercise. Other outcomes will be related to regulation collagen remodelling in skeletal muscle.


Eligibility criteria: Inclusion Criteria:

* Healthy, recreationally active, male and female participants.
* Healthy will be defined as someone who does not have a health condition that would compromise their ability to safely participate in the strenuous physical activity involved in our study as screened for by the Get Active Questionnaire.
* Willing to abstain from resistance exercise for 2 weeks prior to the first metabolic trial and for the duration of the study.
* Participants must be able to abstain from lower-body resistance and/or plyometric exercise for at least 2 weeks prior to the first metabolic trial.
* Participants will be aged 18-35 years old.
* Participants must be normal to overweight (e.g., BMI 18.5-30).
* Participants are willing to abide by the compliance rules of this study (e.g., abstain from physical activity and alcohol 48h prior to each trial).
* Self-reported regular menstrual cycle (25-35d) within the last 3 months (female participants).

Exclusion Criteria:

* Inability to adhere to any of the compliance rules judged by principal investigator or medical doctor.
* Self-reported regular tobacco use.
* Self-reported illicit drug use (e.g. growth hormone, testosterone, etc.).
* Regular use of anti-inflammatory drugs (e.g., ibuprofen).
* Self-reported regularly sleeping less than an average of 7 hours over 3 days (minimum recommended daily amount).
* Individuals who have participated in studies within the past year involving any of the stable isotopes in the study.
* Use of birth control and discontinued use in the last 3-months (female only).

Primary outcomes:
measure: Enrichment of Muscle Collagen
timeFrame: 9 hours
Secondary outcomes:
measure: Area of muscle with localized collagen remodelling
timeFrame: 9 hours
measure: Concentration of Urinary Growth Hormone
timeFrame: 8 hours
measure: Enrichment of Myofibrillar Protein
timeFrame: 9 hours
Other outcomes:
measure: Intensity of Muscle Soreness
timeFrame: 24 hours
Locations:
city: Toronto
country: Canada
facility: Goldring Centre for High Performance Sport at the University of Toronto
state: Ontario
Study officials:
References: