Clinical trial NCT06372262: Effect of 2000-meter Rowing Test on Parameters of Intestinal Integrity in Elite Rowers During Competitive Phase
Official title: Effect of 2000-meter Rowing Test on Parameters of Intestinal Integrity in Elite Rowers During Competitive Phase
Study type: OBSERVATIONAL
Overall status: COMPLETED
Conditions: Endothelial Dysfunction
Keywords: endotoxin
gastrointestinal symptoms
gut permeability
Lead sponsor: Poznan University of Physical Education
Sponsor class: OTHER
First posted: 2024-04-17
Enrollment: 18
Sex: MALE
Minimum age (years): 18
Maximum age (years): 23
Standard ages: ADULT
Accepts healthy volunteers: yes
Observational model: COHORT
Time perspective: RETROSPECTIVE
Sampling method: NON_PROBABILITY_SAMPLE
Study population: Male rowers age 18 - 22 years.

Brief summary: The study aimed to check the 2000m ergometer test on markers of gut permeability in the competitive phase of rowers. 18 members of the Polish rowing team took part in the study.

Detailed description: The phenomenon of epithelial wall leakage has been the subject of investigations within athletic domains such as running and cycling. Nevertheless, there exists an insufficiency of understanding regarding gut permeability in alternative disciplines, such as rowing, particularly following the completion of a standard competitive distance of 2000 meters. Hence, the principal objective of our study was to assess gut permeability after completing a 2000-meter rowing test. The study was performed at the beginning of a competitive training phase. Eighteen elite Polish Rowing Team rowers participated in the study after applying the inclusion/exclusion criteria. The participants performed a 2000-meter ergometer test. Blood samples were taken before the test, after exercise, and after 1-hour of restitution. Parameters, such as I-FABP, LPS, LBP, and zonulin, were determined using appropriate biochemical tests.

Eligibility criteria: Inclusion Criteria:

* minimum five years of training,
* five training sessions per week minimum,
* total training time minimum of 240 minutes,
* membership in the Polish Rowing Team,
* finishing the 2000-meter ergometer test.

Exclusion Criteria:

* antibiotic therapy within the last three months,
* probiotics and prebiotics within the last three months,
* dietary regime,
* gastrointestinal diseases.

Primary outcomes:
measure: I-FABP (intestinal fatty acid binding protein)to measure epithelial wall injury
timeFrame: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period
measure: zonulin to measure tight junction leakage
timeFrame: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period
measure: LBP (lipopolysaccharide binding protein) to measure endotoxin
timeFrame: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period
measure: LPS (lipopolysaccharide) to measure endotoxin
timeFrame: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period
measure: Lactic acid to measure fatigue after the race
timeFrame: Blood samples was taken from earlobe at two-time points: before each stress test (after overnight fasting), one minute after the end of the test.
Secondary outcomes:
measure: energy
timeFrame: day before test
measure: fiber intake
timeFrame: day before test
measure: protein
timeFrame: day before test
measure: carbohydrate
timeFrame: day before test
measure: body mass
timeFrame: in the morning before the test
measure: Body composition - water
timeFrame: in the morning before the test
measure: Body composition - fat
timeFrame: in the morning before the test
measure: Lean body mass
timeFrame: in the morning before the test
Other outcomes:
Locations:
city: Poznań
country: Poland
facility: Poznan University of Physical Education
Study officials:
affiliation: Poznan University of Physical Education
name: Anna Skarpańska-Stejnborn, proffessor
role: STUDY_DIRECTOR
References: