Protein: Carotene epsilon-monooxygenase, chloroplastic (EC 1.14.14.158) (Cytochrome P450 97C1) (Protein LUTEIN DEFICIENT 1) (Q6TBX7)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Heme-containing cytochrome P450 involved in the biosynthesis of xanthophylls. Specific for epsilon- and beta-ring hydroxylation of alpha-carotene. Has only a low activity toward the beta-rings of beta-carotene. The preferred substrate in planta is not alpha-carotene but the epsilon-ring of zeinoxanthin. Possesses a major beta-carotene hydroxylase activity in planta when depleted in its preferred substrate alpha-carotene.
Subcellular location: Plastid, chloroplast {ECO:0000255}.
InterPro domains:
- IPR036396: Cytochrome P450 superfamily (homologous_superfamily) [83-539]
- IPR017972: Cytochrome P450, conserved site (conserved_site) [480-489]
- IPR001128: Cytochrome P450 (family) [102-522]
- IPR002401: Cytochrome P450, E-class, group I (family) [105-510]
- IPR050196: Cytochrome P450 Monooxygenases (family) [103-539]
Sequence:
MESSLFSPSSSSYSSLFTAKPTRLLSPKPKFTFSIRSSIEKPKPKLETNSSKSQSWVSPDWLTTLTRTLSSGKNDESGIPIANAKLDDVADLLGGALFLPLYKWMNEYGPIYRLAAGPRNFVIVSDPAIAKHVLRNYPKYAKGLVAEVSEFLFGSGFAIAEGPLWTARRRAVVPSLHRRYLSVIVERVFCKCAERLVEKLQPYAEDGSAVNMEAKFSQMTLDVIGLSLFNYNFDSLTTDSPVIEAVYTALKEAELRSTDLLPYWKIDALCKIVPRQVKAEKAVTLIRETVEDLIAKCKEIVEREGERINDEEYVNDADPSILRFLLASREEVSSVQLRDDLLSMLVAGHETTGSVLTWTLYLLSKNSSALRKAQEEVDRVLEGRNPAFEDIKELKYITRCINESMRLYPHPPVLIRRAQVPDILPGNYKVNTGQDIMISVYNIHRSSEVWEKAEEFLPERFDIDGAIPNETNTDFKFIPFSGGPRKCVGDQFALMEAIVALAVFLQRLNVELVPDQTISMTTGATIHTTNGLYMKVSQR
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0016491 (oxidoreductase activity), GO:0004497 (monooxygenase activity), GO:0046906 (tetrapyrrole binding), GO:0020037 (heme binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0042440 (pigment metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006629 (lipid metabolic process), GO:0046148 (pigment biosynthetic process), GO:0044255 (cellular lipid metabolic process), GO:0044249 (cellular biosynthetic process), GO:0006720 (isoprenoid metabolic process), GO:0016117 (carotenoid biosynthetic process), GO:0008299 (isoprenoid biosynthetic process), GO:0008610 (lipid biosynthetic process), GO:0006721 (terpenoid metabolic process), GO:0016114 (terpenoid biosynthetic process), GO:0016108 (tetraterpenoid metabolic process), GO:0016109 (tetraterpenoid biosynthetic process), GO:0016116 (carotenoid metabolic process), GO:0016122 (xanthophyll metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0009532 (plastid stroma), GO:0043226 (organelle), GO:0031975 (envelope), GO:0005737 (cytoplasm), GO:0031967 (organelle envelope), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0009536 (plastid), GO:0009570 (chloroplast stroma), GO:0009526 (plastid envelope), GO:0009507 (chloroplast), GO:0043231 (intracellular membrane-bounded organelle), GO:0009941 (chloroplast envelope)