Protein: DExH-box ATP-dependent RNA helicase DExH15 chloroplastic (EC 3.6.4.13) (ATP-dependent RNA helicase ISE2) (Protein EMBRYO DEFECTIVE 25) (Protein INCREASED SIZE EXCLUSION LIMIT 2) (Protein PIGMENT DEFECTIVE 317) (B9DFG3)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: RNA helicase involved in group II intron splicing. Essential protein required during embryogenesis. Involved in post-transcriptional gene silencing. Modulates the determination of cell fate. Necessary for normal plasmodesmata (PD) development and aperture regulation.
Subcellular location: Plastid, chloroplast {ECO:0000269|PubMed:18431481, ECO:0000269|PubMed:22106293, ECO:0000269|PubMed:26147377}. Cytoplasmic granule {ECO:0000269|PubMed:17601829}. Note=Localizes to granule-like structures, probably stress granules (SGs), which number increases upon stress. {ECO:0000269|PubMed:17601829}.
InterPro domains:
- IPR050699: RNA/DNA Helicase (family) [16-1151]
- IPR057416: ISE2-like, SH3 domain (domain) [731-913]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [115-603]
- IPR014001: Helicase superfamily 1/2, ATP-binding domain (domain) [151-341]
- IPR011545: DEAD/DEAH-box helicase domain (domain) [157-313]
- IPR012961: ATP-dependent RNA helicase Ski2/MTR4, C-terminal (domain) [996-1170]
- IPR001650: Helicase, C-terminal domain-like (domain) [424-620]
Sequence:
MNTLPVVSLTASSSFKFFHFPSLHRSLSHSPNFSFTKSLILNPNHLSFKSTLNSLSPSQSQLYEEEDDEEEEEEDEDDDDEAADEYDNISDEIRNSDDDDDDEETEFSVDLPTESARERVEFRWQRVEKLRSLVRDFGVEMIDIDELISIYDFRIDKFQRLAIEAFLRGSSVVVSAPTSSGKTLIAEAAAVSTVAKGRRLFYTTPLKALSNQKFREFRETFGDDNVGLLTGDSAINKDAQIVIMTTEILRNMLYQSVGMASSGTGLFHVDAIVLDEVHYLSDISRGTVWEEIVIYCPKEVQLICLSATVANPDELAGWIGEIHGKTELVTSTRRPVPLTWYFSTKHSLLPLLDEKGINVNRKLSLNYLQLSASEARFRDDDDGYRKRRSKKRGGDTSYNNLVNVTDYPLSKNEINKIRRSQVPQISDTLWHLQGKNMLPAIWFIFNRRGCDAAVQYVENFQLLDDCEKSEVELALKKFRVLYPDAVRESAEKGLLRGIAAHHAGCLPLWKSFIEELFQRGLVKVVFATETLAAGINMPARTAVISSLSKKAGNERIELGPNELYQMAGRAGRRGIDEKGYTVLVQTAFEGAEECCKLVFAGVKPLVSQFTASYGMVLNLVAGSKVTRKSSGTEAGKVLQAGRSLEEAKKLVEKSFGNYVSSNVTVAAKQELAEIDNKIEILSSEISDEAIDKKSRKLLSARDYKEITVLKEELREEKRKRAEQRRRMELERFLALKPLLKGMEEGNLPFICLEFKDSEGREQSVPAVYLGHIDSFQGSKLQKMMSLDESFALNLIEDELAADEPGKPNVKPSYYVALGSDNSWYLFTEKWVRTVYRTGFPNIALALGDALPREIMKNLLDKADMQWDKLAESELGSLWRLEGSLETWSWSLNVPVLSSLSDEDEVLHMSEEYDNAAQKYKEQRSKISRLKKKMSRSEGFREYKKILENANLTVEKMKRLKARSRRLINRLEQIEPSGWKDFMRISNVIHESRALDINTHLIFPLGETAAAIRGENELWLAMVLRNKALVDLKPPQLAGVCASLVSEGIKVRPWRDNNYIYEPSDTVVDMVNFLEDQRSSLIKLQEKHEVMIPCCLDVQFSGMVEAWASGLSWKEMMMECAMDEGDLARLLRRTIDLLAQIPKLPDIDPVLQRSAAAAADIMDRPPISELAG
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0003676 (nucleic acid binding), GO:0003723 (RNA binding), GO:0003729 (mRNA binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0044085 (cellular component biogenesis), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0016043 (cellular component organization), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0022613 (ribonucleoprotein complex biogenesis), GO:0043933 (protein-containing complex organization), GO:0006996 (organelle organization), GO:0010467 (gene expression), GO:0022607 (cellular component assembly), GO:0070925 (organelle assembly), GO:0042254 (ribosome biogenesis), GO:0016070 (RNA metabolic process), GO:0022618 (ribonucleoprotein complex assembly), GO:0065003 (protein-containing complex assembly), GO:0006396 (RNA processing), GO:0071826 (ribonucleoprotein complex subunit organization), GO:0042255 (ribosome assembly), GO:0034470 (ncRNA processing), GO:0140694 (non-membrane-bounded organelle assembly), GO:0006364 (rRNA processing), GO:0034660 (ncRNA metabolic process), GO:0016072 (rRNA metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0009536 (plastid), GO:0009507 (chloroplast), GO:0043231 (intracellular membrane-bounded organelle)