Protein: O-antigen biosynthesis glycosyltransferase WclY (A6M9B7)
Organism: Escherichia coli
Function: Involved in the assembly of the O-repeating unit during O- antigen biosynthesis. Glucosyltransferase accountable for the alpha-D- Glc-1,4-beta-D-Gal linkage within the O-antigen. Transfers alpha-1,4-Glc to the Gal moiety of a specific Gal-beta1-3GalNAc-alpha-OPO3-PO3-phenoxyundecyl (Gal-beta1- 3GalNAc-PP-PhU) synthetic natural acceptor substrate analog. Requires both Gal-beta1-3GalNAc-alpha and the diphosphate moiety in the acceptor. Not active with GalNAc-PP-PhU, GlcNAc-PP-PhU, Gal-beta1- 3GalNAc-alpha-O-benzyl, D-Rha-alpha1-3GlcNAc-alpha-PP-PhU or D-Man- alpha1-3Man-alpha-5-benzamidopentyl (BAP), nor with glycopeptides TTTVTP (Gal-beta1-3GalNAc-alpha-)TPTG or TT (Gal-beta1-3GalNAc- alpha-)TVTPTPTG as acceptor substrates. Has a broad nucleotide sugar donor substrate specificity with ADP-Glc, TDP-Glc and UDP-Glc as superior donors. Gal, GlcNAc, and GalNAc residues are transferred from UDP-sugars, but with low activity. UDP-Xyl, UDP-GlcA, GDP-Fuc or GDP-K- Rha do not act as donors
Subcellular location: Membrane; Single-pass membrane protein
InterPro domains:
- IPR028098: Glycosyltransferase subfamily 4-like, N-terminal domain (domain) [14-161]
- IPR001296: Glycosyl transferase, family 1 (domain) [171-319]
Sequence:
MKIAYVVSSKKKCGPNIVILNIVKELANKHEMEIFFLDESDDDVFECVNVKSTQIKKASDLKEHLKRFDIIHSSGIRPDALVVLCKVIYRVKCKIITTIHNYVFQDLYYSYGLVKSLIGGLLWCSIWLFFDKLVILSKNADNYYWFLPSAKKNIIYNGIDDNDCLQNKKCNYRKEFNIPDDGILAGSCANLTKRKGIDLVIQTLTKEHKIYYIVAGNGIEKHNLINLVKARKLHERVYFIDFLDEPESFMSQLDVFLMPSRSEGFGLTVLESTKLGIPVITSNIPIFMELFDQMCLTFDIKNPSTLIDVITYAKKNRLHLSQKFHAIFQDRFTSSKMATKYENVYNNLFREVL
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016757 (glycosyltransferase activity), GO:0016758 (hexosyltransferase activity), GO:0000030 (mannosyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006629 (lipid metabolic process), GO:0044255 (cellular lipid metabolic process), GO:0044249 (cellular biosynthetic process), GO:0005975 (carbohydrate metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:1901135 (carbohydrate derivative metabolic process), GO:0044262 (cellular carbohydrate metabolic process), GO:0009059 (macromolecule biosynthetic process), GO:0016051 (carbohydrate biosynthetic process), GO:0005976 (polysaccharide metabolic process), GO:0034637 (cellular carbohydrate biosynthetic process), GO:1903509 (liposaccharide metabolic process), GO:0034645 (cellular macromolecule biosynthetic process), GO:1901137 (carbohydrate derivative biosynthetic process), GO:0008610 (lipid biosynthetic process), GO:0008653 (lipopolysaccharide metabolic process), GO:0044264 (cellular polysaccharide metabolic process), GO:0046402 (O antigen metabolic process), GO:0009103 (lipopolysaccharide biosynthetic process), GO:0033692 (cellular polysaccharide biosynthetic process), GO:0000271 (polysaccharide biosynthetic process), GO:0009243 (O antigen biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0016020 (membrane)