Protein: Ribosome biogenesis protein NOP53 (Glioma tumor suppressor candidate region gene 2 protein) (PreS1-binding protein) (Q8BK35)
Organism: Mus musculus (Mouse)
Function: Nucleolar protein which is involved in the integration of the 5S RNP into the ribosomal large subunit during ribosome biogenesis. In ribosome biogenesis, may also play a role in rRNA transcription (By similarity). Also functions as a nucleolar sensor that regulates the activation of p53/TP53 in response to ribosome biogenesis perturbation, DNA damage and other stress conditions. DNA damage or perturbation of ribosome biogenesis disrupt the interaction between NOP53 and RPL11 allowing RPL11 transport to the nucleoplasm where it can inhibit MDM2 and allow p53/TP53 activation. It may also positively regulate the function of p53/TP53 in cell cycle arrest and apoptosis through direct interaction, preventing its MDM2-dependent ubiquitin-mediated proteasomal degradation. Originally identified as a tumor suppressor, it may also play a role in cell proliferation and apoptosis by positively regulating the stability of PTEN, thereby antagonizing the PI3K-AKT/PKB signaling pathway. May also inhibit cell proliferation and increase apoptosis through its interaction with NF2. May negatively regulate NPM1 by regulating its nucleoplasmic localization, oligomerization and ubiquitin-mediated proteasomal degradation. Thereby, may prevent NPM1 interaction with MYC and negatively regulate transcription mediated by the MYC-NPM1 complex. May also regulate cellular aerobic respiration. In the cellular response to viral infection, may play a role in the attenuation of interferon-beta through the inhibition of RIGI (By similarity).
Subcellular location: Nucleus, nucleolus {ECO:0000269|PubMed:21804542}. Nucleus, nucleoplasm {ECO:0000250|UniProtKB:Q9NZM5}. Note=In the nucleolus may be more specifically localized to the fibrillar center. Mainly nucleolar it relocalizes to the nucleoplasm under specific conditions including ribosomal stress enabling it to interact and regulate nucleoplasmic proteins like p53/TP53. Also detected in the cytosol. {ECO:0000250|UniProtKB:Q9NZM5}.
InterPro domains:
- IPR011687: Ribosome biogenesis protein Nop53/GLTSCR2 (family) [1-484]
Sequence:
MAAGGNRDGEKRGSRSQADSGFLGLRPTSVDPALRRRRRGPRNKKRGWRRLAEEPLGLEVDQFLEDVRLQERTTGGLLAEAPNEKLFFVDTGFKRKEPRKKRTLVQKKSQRLQKPLRVDLALENHSKIPAPKDILAHQVPNAKKLRRKEELWEKLAKQGELPRDVRKAQARLLSPPTPKAKPGPQDIIERPFYDLWNPDNPLDTPLIGQDAFFLEQTKKKGVRRPQRLHIKPSQVPAVEVIPAGASYNPTFEDHQALLREAHEVELQREKEAEKLERQLALPTSEQAATQESVFREMCEGLLEESDGEDEHEAGRAGQPEAGDGTTEISPTGAAGPEKRMEKKTEQQRRREKAARKLRVQQAALRAARLQHQELFRLRGIKAQVARRLAELARRREQRRIRRLAEADKPRRLGRLKYQAPDIDVQLSSELSGSLRTLKPEGHILRDRFKSFQKRNMIEPRERAKFKRKYKVKLVEKRAYREIQL
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0051179 (localization), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0065007 (biological regulation), GO:0009987 (cellular process), GO:0051641 (cellular localization), GO:0051640 (organelle localization), GO:0023056 (positive regulation of signaling), GO:0031503 (protein-containing complex localization), GO:0048584 (positive regulation of response to stimulus), GO:0019222 (regulation of metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0051234 (establishment of localization), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0033036 (macromolecule localization), GO:0050794 (regulation of cellular process), GO:0048583 (regulation of response to stimulus), GO:0009893 (positive regulation of metabolic process), GO:0023051 (regulation of signaling), GO:0051640 (organelle localization), GO:0048522 (positive regulation of cellular process), GO:0009967 (positive regulation of signal transduction), GO:0044085 (cellular component biogenesis), GO:0010604 (positive regulation of macromolecule metabolic process), GO:0051656 (establishment of organelle localization), GO:0046483 (heterocycle metabolic process), GO:0009966 (regulation of signal transduction), GO:0051649 (establishment of localization in cell), GO:0060255 (regulation of macromolecule metabolic process), GO:0033750 (ribosome localization), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0009891 (positive regulation of biosynthetic process), GO:1901360 (organic cyclic compound metabolic process), GO:0046907 (intracellular transport), GO:0006139 (nucleobase-containing compound metabolic process), GO:0031325 (positive regulation of cellular metabolic process), GO:0010646 (regulation of cell communication), GO:0051656 (establishment of organelle localization), GO:0009889 (regulation of biosynthetic process), GO:0051171 (regulation of nitrogen compound metabolic process), GO:0051173 (positive regulation of nitrogen compound metabolic process), GO:0070727 (cellular macromolecule localization), GO:0031323 (regulation of cellular metabolic process), GO:0010647 (positive regulation of cell communication), GO:0080090 (regulation of primary metabolic process), GO:0000054 (ribosomal subunit export from nucleus), GO:0010556 (regulation of macromolecule biosynthetic process), GO:0022613 (ribonucleoprotein complex biogenesis), GO:0051169 (nuclear transport), GO:0010468 (regulation of gene expression), GO:0010557 (positive regulation of macromolecule biosynthetic process), GO:0045935 (positive regulation of nucleobase-containing compound metabolic process), GO:0051254 (positive regulation of RNA metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0031326 (regulation of cellular biosynthetic process), GO:0019219 (regulation of nucleobase-containing compound metabolic process), GO:1902533 (positive regulation of intracellular signal transduction), GO:0051252 (regulation of RNA metabolic process), GO:0008104 (protein localization), GO:0010467 (gene expression), GO:0031328 (positive regulation of cellular biosynthetic process), GO:1902531 (regulation of intracellular signal transduction), GO:0006913 (nucleocytoplasmic transport), GO:0033365 (protein localization to organelle), GO:0016070 (RNA metabolic process), GO:0042273 (ribosomal large subunit biogenesis), GO:2001141 (regulation of RNA biosynthetic process), GO:0006355 (regulation of DNA-templated transcription), GO:0006396 (RNA processing), GO:0042254 (ribosome biogenesis), GO:1902680 (positive regulation of RNA biosynthetic process), GO:1901796 (regulation of signal transduction by p53 class mediator), GO:0006357 (regulation of transcription by RNA polymerase II), GO:0034504 (protein localization to nucleus), GO:0051170 (import into nucleus), GO:0045893 (positive regulation of DNA-templated transcription), GO:1903320 (regulation of protein modification by small protein conjugation or removal), GO:1903508 (positive regulation of nucleic acid-templated transcription), GO:0051168 (nuclear export), GO:1903506 (regulation of nucleic acid-templated transcription), GO:0045944 (positive regulation of transcription by RNA polymerase II)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0031974 (membrane-enclosed lumen), GO:0043229 (intracellular organelle), GO:0043233 (organelle lumen), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0031981 (nuclear lumen), GO:0005730 (nucleolus), GO:0005634 (nucleus)