Protein: CCA tRNA nucleotidyltransferase, mitochondrial (EC 2.7.7.72) (CCA-adding enzyme) (tRNA CCA-pyrophosphorylase) (tRNA adenylyltransferase) (tRNA nucleotidyltransferase) (Q9P4S5)
Organism: Candida glabrata (strain ATCC 2001 / BCRC 20586 / JCM 3761 / NBRC 0622 / NRRL Y-65 / CBS 138) (Yeast) (Nakaseomyces glabratus)
Function: This enzyme carries out synthesis of the tRNA CCA terminus without the direction of a template using the multiple accepting and donating subsites within its active site.
Subcellular location: Mitochondrion {ECO:0000250|UniProtKB:P21269}. Cytoplasm {ECO:0000250|UniProtKB:P21269}. Nucleus {ECO:0000250|UniProtKB:P21269}.
InterPro domains:
- IPR032828: tRNA nucleotidyltransferase/poly(A) polymerase, RNA and SrmB- binding domain (domain) [238-275]
- IPR043519: Nucleotidyltransferase superfamily (homologous_superfamily) [8-185]
- IPR002646: Poly A polymerase, head domain (domain) [18-189]
Sequence:
MFKAIRRVFTMIPRIQLTEKETRICNLLKDYTAHYNSLHYGQEPLTLRITGGWVRDKLLGQGSHDLDIAINIMSGEEFATGLNGYLLEHFDKYGVKPHSIHKIDKNPEKSKHLETATTKLFDVEVDFVNLRSEEYTEDSRIPTTQFGTPEEDALRRDATLNALFYNIQQDAVEDFTKRGWQDLQDGVLRTPLPARQTFLDDPLRVLRLIRFASRFNFNIEAGVLKEMHDPEINEAFNNKISRERIGVEMEKILVGPNPILGLKLIQRTHLENVIFLWHGDQSVIEYNRKNWPQTKDVEDIYKKGIFNHHLKNFIHHYKDFLSRYLKLRQAIETKDKSFQQNFLLASILIPMADLKIIALPKKKLNNTLPVSESIVREGLKFNKASSIVVARCVENIAAYNSMVEKYLQSGDLKRSEVGTFLRELRGDWEIVHYVSLMDQYLKYISRKDNVVNIIDKYDRFWNYIQEQNLQDSDKMVPIIDGKRMVKILETKPGPWLGKINDEVILWQFDHPQGTEQELISFIKSILPNYLQ
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0016740 (transferase activity), GO:0140098 (catalytic activity, acting on RNA), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0140101 (catalytic activity, acting on a tRNA), GO:0016779 (nucleotidyltransferase activity), GO:0070567 (cytidylyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0046483 (heterocycle metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0010467 (gene expression), GO:0016070 (RNA metabolic process), GO:0006396 (RNA processing), GO:0034470 (ncRNA processing), GO:0031123 (RNA 3'-end processing), GO:0034660 (ncRNA metabolic process), GO:0042780 (tRNA 3'-end processing), GO:0006399 (tRNA metabolic process), GO:0008033 (tRNA processing)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0005739 (mitochondrion), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)