Protein: Activated Cdc42 kinase Ack (EC 2.7.10.2) (EC 2.7.11.1) (Q9VZI2)
Organism: Drosophila melanogaster (Fruit fly)
Function: Non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in diverse biological functions such as cell survival, cell differentiation, cell growth and proliferation. Phosphorylates SH3PX1 and ex. Phosphorylates SH3PX1 predominantly on 'Tyr-56', which likely promotes the recruitment of SH3PX1 to an axonal guidance receptor complex that includes dock and Dscam; because phosphorylation of SH3PX1 increases its interaction with the complex member dock while decreasing its interaction with the actin cytoskeleton modulator WASp. In the wing and eye, promotes tissue growth, and during embryogenesis coordinates cell shape changes required for correct dorsal closure. Functions in the negative regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway by enhancing yki activity thereby promoting cell proliferation and inhibiting apoptosis. This is accomplished, at least in part, by phosphorylating ex thereby reducing its ability to efficiently activate the Hippo signaling cascade. In the eye disk, wing disk and possibly spermatids, inhibits programmed cell death induced by hid and rpr through a mechanism that is independent of the MAP kinase signal transduction pathway. Essential for male and female fertility. During oogenesis required for the correct temporal assembly, and consequently the catalytic activity of long CTPsyn filaments (cytoophidium) in the germline nurse cells, likely by phosphorylating an unidentified substrate that is essential for linking individual CTPsyn filaments into large, catalytically active assemblies.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:22615583, ECO:0000269|PubMed:25223282}. Cytoplasmic vesicle, clathrin-coated vesicle {ECO:0000269|PubMed:23562806}. Note=In nurse cells localizes to cytoophidium, a subcellular filamentary structure where CTP synthase (Ctps) is compartmentalized (PubMed:25223282). In third instar photoreceptor R-cells, expressed in the cytoplasm and cytoplasmic puncta (PubMed:22615583). In spermatocytes displays localization to peripheral clathrin-positive structures and localization to some secretory clathrin (PubMed:23562806). {ECO:0000269|PubMed:22615583, ECO:0000269|PubMed:23562806, ECO:0000269|PubMed:25223282}.
InterPro domains:
- IPR011009: Protein kinase-like domain superfamily (homologous_superfamily) [117-391]
- IPR015940: Ubiquitin-associated domain (domain) [1029-1072]
- IPR050198: Non-receptor tyrosine kinases involved in cell signaling (family) [92-392]
- IPR001245: Serine-threonine/tyrosine-protein kinase, catalytic domain (domain) [124-383]
- IPR049587: TNK(ACK)-like non-receptor tyrosine-protein kinases, SAM domain (domain) [14-75]
- IPR017441: Protein kinase, ATP binding site (binding_site) [129-156]
- IPR000719: Protein kinase domain (domain) [123-383]
- IPR008266: Tyrosine-protein kinase, active site (active_site) [246-258]
- IPR055175: ACK/TNK-like, SAM domain (domain) [18-73]
- IPR020635: Tyrosine-protein kinase, catalytic domain (domain) [123-383]
Sequence:
MTSTSAVDGGLGSETAWLEDLLREVQLEQFLDRIRDDLQVTRLAHFDYVLPDDLERCGLGKPAIRRLMEAVRKKKAHQWRKNILSKLIGGGKQPSSKKQSSAARESSQGNGTQLTCLIHEKDITMGLKLGDGSFGVVRRGEWSASPAGKVIPVAVKVLKSDNLTQPGIIDDFFREVQAMHALDHANLVRLYGVVLSQPMMMITELAERGSLLDTLRKQCRHTSLTIIWNWSVQIVTGMAYLEQKRFLHRDLACRNVLLAAGNKIKIGDFGLMRALPQEDDCYVMSEHKKVPFPWCAPESLRFRQFSHASDTWMFGVTLWEMFSFGEDPWVGLNGSQILRKIDREGERLHQPDACPPDVYAMMLQCWDKTPAERPTFAALKEYLASMSPPVMRASRSHHESKGLQIEPGDTIAIIDGRHELKLIKGQNQRTFDIGIFPRNLLEQRKVGAAGDVVMRSSVGNGSSSSPFGFCWGGAAAMANGDDRQRKCASMTNQPHAKERKSTSSKQFAYNKLVNDSATGLQRRNAVKHKGVVVGPQRPPPPQFQQEGILIDISPDMRPIAEAGTGGAKGAGDSSSLQADSSFCILDAPIDVPTYAGSSGSGDLNVSPTYYNEQPQFDFDPAKMTASPGRLQPPPYQMPPTYSNTMEFVQKRDLHQQQLATPVRERDPFDTTNVETTVALYSNFNQSLEAASPPAPIYNSPSVRKSLFGGSKSNKENIPALESAAMQLNLSNLTLERHDATCIQPVEPVPAPPGDGVLLDKSFIAELEKDMYSNGQNRAQEEYQRNSTQMYASKDMVYKQNLTPLKNGAAPGSVHSNHSSPSSTASPKQNNVEAAAAAAATTQSVVNRIWYEQVASTQSEYYAQPPTEQAEEQIYQNHRHQQQQQQELNHSFVAISNRVVAPKNNAYSSTASLYDAVAASTAGSTYYGQVPNGSGAVLYDEVTQDDYLRPTRPAPLAPPPLSAQQIQRRMEKMRLQQQQQLDGAHQLYAPVPSDYGREQEKLQQLMQELGSSAVEQDVRNALRAASGDVGLATRHYKIDQLARLGVAGRPQCEQALQQTNWSLEVAAELLLNAG
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0005515 (protein binding), GO:0004672 (protein kinase activity), GO:0005102 (signaling receptor binding), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0016773 (phosphotransferase activity, alcohol group as acceptor), GO:0070851 (growth factor receptor binding), GO:0016301 (kinase activity), GO:0005154 (epidermal growth factor receptor binding)
Biological Process (BP): GO:0008150 (biological process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0032501 (multicellular organismal process), GO:0048869 (cellular developmental process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0009653 (anatomical structure morphogenesis), GO:0050794 (regulation of cellular process), GO:0071840 (cellular component organization or biogenesis), GO:0030029 (actin filament-based process), GO:0048522 (positive regulation of cellular process), GO:0032989 (cellular component morphogenesis), GO:0000902 (cell morphogenesis), GO:0048468 (cell development), GO:0030154 (cell differentiation), GO:0016358 (dendrite development), GO:0048731 (system development), GO:0030155 (regulation of cell adhesion), GO:0016043 (cellular component organization), GO:0045785 (positive regulation of cell adhesion), GO:0030036 (actin cytoskeleton organization), GO:0010811 (positive regulation of cell-substrate adhesion), GO:0022008 (neurogenesis), GO:0000904 (cell morphogenesis involved in differentiation), GO:0030030 (cell projection organization), GO:0007399 (nervous system development), GO:0010810 (regulation of cell-substrate adhesion), GO:0032990 (cell part morphogenesis), GO:0048813 (dendrite morphogenesis), GO:0006996 (organelle organization), GO:0048858 (cell projection morphogenesis), GO:0030182 (neuron differentiation), GO:0097435 (supramolecular fiber organization), GO:0048666 (neuron development), GO:0007015 (actin filament organization), GO:0007010 (cytoskeleton organization), GO:0048667 (cell morphogenesis involved in neuron differentiation), GO:0031175 (neuron projection development), GO:0001954 (positive regulation of cell-matrix adhesion), GO:0048699 (generation of neurons), GO:0120039 (plasma membrane bounded cell projection morphogenesis), GO:0120036 (plasma membrane bounded cell projection organization), GO:0048812 (neuron projection morphogenesis)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0071944 (cell periphery), GO:0043229 (intracellular organelle), GO:0005938 (cell cortex), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)