Protein: Phospholipase A and acyltransferase 5 (Ca(2+)-independent N-acyltransferase) (iNAT) (EC 2.3.1.-, EC 3.1.1.32, EC 3.1.1.4) (H-rev107-like protein 5) (HRAS-like suppressor 5) (HRSL5) (Rat LRAT-like protein-1) (RLP-1) (Q4KLN5)
Organism: Rattus norvegicus (Rat)
Function: Exhibits both phospholipase A1/2 and acyltransferase activities (By similarity). Shows phospholipase A1 (PLA1) and A2 (PLA2) activity, catalyzing the calcium-independent release of fatty acids from the sn-1 or sn-2 position of glycerophospholipids (By similarity). Shows N-acyltransferase activity, catalyzing the calcium-independent transfer of a fatty acyl group at the sn-1 position of phosphatidylcholine (PC) and other glycerophospholipids to the primary amine of phosphatidylethanolamine (PE), forming N-acylphosphatidylethanolamine (NAPE), which serves as precursor for N-acylethanolamines (NAEs).
Subcellular location: Cytoplasm, cytosol {ECO:0000269|PubMed:17158102}.
InterPro domains:
- IPR051496: H-rev107 Phospholipase/Acyltransferase (family) [130-279]
- IPR007053: LRAT domain (domain) [138-257]
Sequence:
MIPGHRGPWSAPLCRRLVPAGHSGMGLSPAASGEYGIRLFRVPWPSRPKQISRTASTESSDTQPTNDSASSQALVVQFLPKLPKQDRGLEQARSLRQGQKPEINLELIPSKKRTEMIPSSDSEIEGNLKNQAAESNQKPRPGDLIEIFRIGYEHWAIYVEDDCVVHLAPPSEFEAGSITSIFSNRAVVKYSRLQDVLHGCSWKINNKLDGTYLPLPVDKIIQRTKNMINKIVQYSLIEGNCEHFVNDLRYGVPRSQQVEHVLVEGAKAAGAVLSAVVDSIRPKPITA
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016746 (acyltransferase activity), GO:0016747 (acyltransferase activity, transferring groups other than amino-acyl groups), GO:0016410 (N-acyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0044255 (cellular lipid metabolic process), GO:0043603 (amide metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0006629 (lipid metabolic process), GO:0019637 (organophosphate metabolic process), GO:0006793 (phosphorus metabolic process), GO:0006796 (phosphate-containing compound metabolic process), GO:0046486 (glycerolipid metabolic process), GO:0006644 (phospholipid metabolic process), GO:0006650 (glycerophospholipid metabolic process), GO:0046337 (phosphatidylethanolamine metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0048471 (perinuclear region of cytoplasm), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0005773 (vacuole), GO:0005739 (mitochondrion), GO:0005783 (endoplasmic reticulum), GO:0031410 (cytoplasmic vesicle), GO:0043229 (intracellular organelle), GO:0005768 (endosome), GO:0043227 (membrane-bounded organelle), GO:0005791 (rough endoplasmic reticulum), GO:0000323 (lytic vacuole), GO:0043231 (intracellular membrane-bounded organelle), GO:0031982 (vesicle), GO:0005764 (lysosome), GO:0097708 (intracellular vesicle)