Protein: Minor capsid protein VP2 (Minor structural protein VP2) (P03093)
Organism: Simian virus 40 (SV40)
Function: Structural protein that resides within the core of the capsid surrounded by 72 VP1 pentamers. Following virus endocytosis and trafficking to the endoplasmic reticulum, VP2 and VP3 form oligomers and integrate into the endoplasmic reticulum membrane. Heterooligomer VP2-VP3 may create a viroporin for transporting the viral genome across the endoplasmic reticulum membrane to the cytoplasm. Nuclear entry of the viral DNA involves the selective exposure and importin recognition of VP2 or VP3 nuclear localization signal (shared C-terminus). Plays a role in virion assembly within the nucleus in particular through a DNA-binding domain located in the C-terminal region. A N-terminal myristoylation suggests a scaffold function for virion assembly. The viral progenies exit the cells by lytic release. Isoform VP2 may repress SP1 activation of the SV40 early promoter, via specific protein-protein and protein-DNA interactions.
Subcellular location: [Isoform VP2]: Virion {ECO:0000305}. Host nucleus {ECO:0000269|PubMed:1847270}. Host endoplasmic reticulum {ECO:0000269|PubMed:17189196}. Host endoplasmic reticulum membrane {ECO:0000269|PubMed:17189196}. Note=Following host cell entry, the virion enters into the endoplasmic reticulum through a calveolar-dependent pathway. Then, isoform VP2 integrates into the endoplasmic reticulum membrane and participates in the translocation of viral DNA to the nucleus. Shortly after synthesis, a nuclear localization signal directs isoform VP2 to the cell nucleus where virion assembly occurs. {ECO:0000269|PubMed:17189196, ECO:0000269|PubMed:1847270}.; SUBCELLULAR LOCATION: [Isoform VP3]: Virion {ECO:0000305}. Host nucleus {ECO:0000269|PubMed:1847270}. Host endoplasmic reticulum {ECO:0000269|PubMed:17189196}. Host endoplasmic reticulum membrane {ECO:0000269|PubMed:17189196, ECO:0000269|PubMed:25631089}. Note=Following host cell entry, the virion enters into the endoplasmic reticulum through a calveolar-dependent pathway. Then, isoform VP3 integrates into the endoplasmic reticulum membrane and participates in the translocation of viral DNA to the nucleus. Shortly after synthesis, a nuclear localization signal directs isoform VP3 to the cell nucleus where virion assembly occurs. {ECO:0000269|PubMed:17189196, ECO:0000269|PubMed:1847270}.; SUBCELLULAR LOCATION: [Isoform VP4]: Host nucleus {ECO:0000269|PubMed:17658947}.
InterPro domains:
- IPR001070: Polyomavirus coat protein VP2 (family) [1-352]
Sequence:
MGAALTLLGDLIATVSEAAAATGFSVAEIAAGEAAAAIEVQLASVATVEGLTTSEAIAAIGLIPQAYAVISGAPAAIAGFAALLQTVTGVSAVAQVGYRFFSDWDHKVSTVGLYQQPGMAVDLYRPDDYYDILFPGVQTFVHSVQYLDPRHWGPTLFNAISQAFWRVIQNDIPRLTSQELERRTQRYLRDSLARFLEETTWTVINAPVNWYNSLQDYYSTLSPIRPTMVRQVANREGLQISFGHTYDNIDEADSIQQVTERWEAQSQSPNVQSGEFIEKFEAPGGANQRTAPQWMLPLLLGLYGSVTSALKAYEDGPNKKKRKLSRGSSQKTKGTSASAKARHKRRNRSSRS
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0016032 (viral process), GO:0044419 (biological process involved in interspecies interaction between organisms), GO:0044068 (modulation by symbiont of host cellular process), GO:0019048 (modulation by virus of host process), GO:0044403 (biological process involved in symbiotic interaction), GO:0035821 (modulation of process of another organism), GO:0051701 (biological process involved in interaction with host), GO:0019054 (modulation by virus of host cellular process), GO:0044003 (modulation by symbiont of host process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0043229 (intracellular organelle), GO:0043233 (organelle lumen), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0031981 (nuclear lumen), GO:0005634 (nucleus)