Protein: Alpha-thujene synthase TPS3, chloroplastic (EC 4.2.3.-) ((+)-sabinene synthase TPS3) (EC 4.2.3.110) (Terpene synthase 3) (LcTPS3) (G0Y7D3)
Organism: Litsea cubeba (Aromatic litsea) (Laurus cubeba)
Function: Monoterpene synthase (TPS) involved in the biosynthesis of monoterpene natural products used by traditional Chinese medicine to treat headache, inflammation and intoxication (Ref.1). Catalyzes the conversion of (2E)-geranyl diphosphate (GPP) into alpha-thujene and (1R,5R)-sabinene (Ref.1).
Subcellular location: Plastid, chloroplast {ECO:0000255}.
InterPro domains:
- IPR008930: Terpenoid cyclases/protein prenyltransferase alpha-alpha toroid (homologous_superfamily) [52-251]
- IPR005630: Terpene synthase, metal-binding domain (domain) [284-522]
- IPR001906: Terpene synthase, N-terminal domain (domain) [55-227]
- IPR008949: Isoprenoid synthase domain superfamily (homologous_superfamily) [53-576]
- IPR034741: Terpene cyclase-like 1, C-terminal domain (domain) [253-577]
- IPR050148: Terpene synthase-like (family) [45-560]
- IPR044814: Terpene cyclases, class 1, plant (family) [46-576]
- IPR036965: Terpene synthase, N-terminal domain superfamily (homologous_superfamily) [92-253]
Sequence:
MALQLLTPSFSFQHSPSPHRLTTLRYTHHTIRCTASAPSYSDLVGRRSANYKPSKWDSNFVETLESDYKKENHEMYIEKLMGDVKHLMKKVVNPIEKMELVDTIQRLGLGYLFNKEIKEVLNTIATSKATFKTKKDLHAVALQFRLLRQHGYEVSPDAFHKFKDEKGGFKESLCMDIKGMLSLYEASHLSFQGEVVLDEAREFTSTHLKAIEGNIDPVLLKKVRHSLEMPLHWRMLRLEARWYIETYDEEDRKNPSLAELAKHDFNSVQTIYQRSLKRMSRWWRDLGLGERLEFSRDRLVECFFWTTGVIFDPQFERCRGVLTKVNQLVSTIDDVYDVYGSLEELELFTDAVDRWDIRAMEQLPEYMKICYLALYNTTNDIAYEALKEEGLDVIPYLKKVWTDLCKSYIVEARWYSNGYKPTLEEYLENAWTSIAGPVALGHAYFSFGQKMPFEALNYSNTSSLIKWSSMIFRLCDDLATSSDEVARGDVPKSIQCYMYEAGVSESVARDHIKYLIDEAWKKMNECLVPSTPFLQPLINAGFNLARMAHCMYEHGDGHGFSNELDKKRVLLLLAEPFKFM
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016829 (lyase activity), GO:0016835 (carbon-oxygen lyase activity), GO:0016838 (carbon-oxygen lyase activity, acting on phosphates)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044281 (small molecule metabolic process), GO:0044238 (primary metabolic process), GO:0044255 (cellular lipid metabolic process), GO:0044249 (cellular biosynthetic process), GO:0120252 (hydrocarbon metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006629 (lipid metabolic process), GO:0006082 (organic acid metabolic process), GO:0006631 (fatty acid metabolic process), GO:0006720 (isoprenoid metabolic process), GO:0042214 (terpene metabolic process), GO:0120251 (hydrocarbon biosynthetic process), GO:0008299 (isoprenoid biosynthetic process), GO:0043436 (oxoacid metabolic process), GO:0008610 (lipid biosynthetic process), GO:0019752 (carboxylic acid metabolic process), GO:0043692 (monoterpene metabolic process), GO:0019372 (lipoxygenase pathway), GO:0046246 (terpene biosynthetic process), GO:0043693 (monoterpene biosynthetic process), GO:0010597 (green leaf volatile biosynthetic process), GO:0032787 (monocarboxylic acid metabolic process), GO:0006690 (icosanoid metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0009532 (plastid stroma), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0009536 (plastid), GO:0009570 (chloroplast stroma), GO:0009507 (chloroplast), GO:0043231 (intracellular membrane-bounded organelle)