Protein: Endo-1,4-beta-xylanase Xyn11E (EC 3.2.1.8) (V9TXH2)
Organism: Paenibacillus barcinonensis
Function: Involved in depolymerization of xylan, a major component of the lignocellulosic substrates. Acts as an endo-xylanase that efficiently hydrolyzes the beta-1,4 glycosidic linkages between the xylopyranosyl residues in the main chain of the polymer, leading to the degradation of xylan into short oligosaccharides. Shows high activity toward branched xylans from both softwoods (arabinoxylans) and hardwoods (glucuronoxylans), showing the highest activity on beechwood xylan. Also hydrolyzes long xylooligosaccharides (with a degree of polymerization of greater than or equal to 5), while oligomers shorter than xylotetraose are not degraded. Is not active on carboxymethyl cellulose (CMC), Avicel, starch, polygalacturonic acid, laminarin, pectin, beta-D-barley glucan, or lichenan.
Subcellular location: Secreted {ECO:0000305|PubMed:24549767}.
InterPro domains:
- IPR033123: Glycosyl hydrolase 11 domain (domain) [18-210]
- IPR013320: Concanavalin A-like lectin/glucanase domain superfamily (homologous_superfamily) [31-210]
- IPR001137: Glycoside hydrolase family 11 (family) [25-209]
- IPR033119: Glycoside hydrolase family 11, active site 2 (active_site) [194-205]
- IPR013319: Glycoside hydrolase family 11/12 (homologous_superfamily) [25-210]
- IPR018208: Glycoside hydrolase family 11, active site 1 (active_site) [101-111]
Sequence:
MFKFGKKLMTVVLAASMSFGVFAATTGATDYWQNWTDGGGTVNAVNGSGGNYSVNWQNTGNFVVGKGWTYGTPNRVVNYNAGVFSPSGNGYLTFYGWTRNALIEYYVVDNWGTYRPTGTYKGTVNSDGGTYDIYTTMRYNQPSIDGYSTFPQYWSVRQSKRPIGVNSQITFQNHVNAWASKGMNLGSSWSYQVLATEGYQSSGSSNVTVW
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0016798 (hydrolase activity, acting on glycosyl bonds), GO:0004553 (hydrolase activity, hydrolyzing O-glycosyl compounds), GO:0097599 (xylanase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0071554 (cell wall organization or biogenesis), GO:0009056 (catabolic process), GO:0044238 (primary metabolic process), GO:0044036 (cell wall macromolecule metabolic process), GO:0005975 (carbohydrate metabolic process), GO:1901575 (organic substance catabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0044262 (cellular carbohydrate metabolic process), GO:0044248 (cellular catabolic process), GO:0009057 (macromolecule catabolic process), GO:0010383 (cell wall polysaccharide metabolic process), GO:0016998 (cell wall macromolecule catabolic process), GO:0016052 (carbohydrate catabolic process), GO:0044265 (cellular macromolecule catabolic process), GO:0044264 (cellular polysaccharide metabolic process), GO:0005976 (polysaccharide metabolic process), GO:0000272 (polysaccharide catabolic process), GO:0044347 (cell wall polysaccharide catabolic process), GO:0010410 (hemicellulose metabolic process), GO:2000895 (hemicellulose catabolic process), GO:0045491 (xylan metabolic process), GO:0045493 (xylan catabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0018995 (host cellular component), GO:0005576 (extracellular region), GO:0043657 (host cell), GO:0033643 (host cell part), GO:0033646 (host intracellular part), GO:0043656 (host intracellular region), GO:0030430 (host cell cytoplasm), GO:0033655 (host cell cytoplasm part), GO:0044164 (host cell cytosol)