Protein: Tubulin polyglutamylase TTLL7 (EC 6.3.2.-) (Tubulin--tyrosine ligase-like protein 7) (mTTLL7) (A4Q9F0)
Organism: Mus musculus (Mouse)
Function: Polyglutamylase which modifies tubulin, generating polyglutamate side chains of variable lengths on the gamma-carboxyl group of specific glutamate residues within the C-terminal tail of tubulin. Mediates both ATP-dependent initiation and elongation steps of the polyglutamylation reaction. Preferentially modifies the beta-tubulin tail over an alpha-tail. Competes with monoglycylase TTLL3 for modification site on beta-tubulin substrate, thereby creating an anticorrelation between glycylation and glutamylation reactions (By similarity). Required for neurite growth; responsible for the strong increase in tubulin polyglutamylation during postnatal neuronal maturation.
Subcellular location: Cell projection, cilium {ECO:0000269|PubMed:16901895, ECO:0000269|PubMed:17499049}. Cytoplasm, cytoskeleton, cilium basal body {ECO:0000269|PubMed:16901895, ECO:0000269|PubMed:17499049}. Cell projection, dendrite {ECO:0000269|PubMed:16901895, ECO:0000269|PubMed:17499049}. Perikaryon {ECO:0000269|PubMed:16901895, ECO:0000269|PubMed:17499049}. Note=In cells with primary cilia, found in both cilia and basal bodies. In neuronal cells, found in dendrites and perikaryon. {ECO:0000269|PubMed:16901895, ECO:0000269|PubMed:17499049}.
InterPro domains:
- IPR004344: Tubulin-tyrosine ligase/Tubulin polyglutamylase (family) [38-390]
Sequence:
MPSLPQDGVIQGSSPVDLGTELPYQCTMKRKVRKKKKKGIITANVAGTKFEIVRLVIDEMGFMKTPDEDETSNLIWCDAAVQQEKITDLQNYQRINHFPGMGEICRKDFLARNMTKMIKSRPMDYTFVPRTWIFPSEYTQFQNYVKELKKKRKQKTFIVKPANGAMGHGISLIRNGDKVPSQDHLIVQEYIEKPFLMEGYKFDLRIYILVTSCDPLKIFLYHDGLVRMGTEKYIPPNESNLTQLYMHLTNYSVNKHNERFERNETEDKGSKRSIKWFTEFLQANQHDVTKFWSDISELVVKTLIVAEPHVLHAYRMCRPGQPPGSESVCFEVLGFDILLDRKLKPWLLEINRAPSFGTDQKIDYDVKRGVLLNALKLLNIRTSDKRKNLAKQKAEAQRRLYGQNPVRRLSPGSSDWEQQRHQLERRKEELKERLLQVRKQVSQEEHENRHMGNYRRIYPPEDKALLEKYEGLLAVAFQTFLSGRAASFQREMNNPLKKMREEDLLDLLEQCEIDDEKLMGKTGRVRGPKPLCCMPECAEVTKKQKYYGSSDSSYDSSSSSSNSELDENEKELCQKRLDQVPYSLKHTSHCKIIQQPSGSHNLIYSESPVYLTTLVFLSEFPDSMRRSVSCPRSISAHLPSRGDVRPFSSQQVIPLARPTSASRSHSLNRASSYARHLPHGSDTGSTNTLNESLRQLKTKEQEDDLTSQTLFVLKDMRIRFPGKSDAESELLIEDIMDNWKHYKTKVASYWLIKLDSVKQRKVLDIVKSSIRTVLPRIWRVPDAEELSLYRIFNRVFNRLLWSHGQGLWSCFCDSGSSWESIFSKSPEVVTPLQLQCCQRLVELCKQCLLVVYKYTTETRGPISGIGPDWGNSRYLLPGSTQFLMRSPLYNMKYNSPGMTRSNVLFTSRYGRL
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016874 (ligase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0005515 (protein binding), GO:0070739 (protein-glutamic acid ligase activity), GO:0016879 (ligase activity, forming carbon-nitrogen bonds), GO:0008092 (cytoskeletal protein binding), GO:0016881 (acid-amino acid ligase activity), GO:0070740 (tubulin-glutamic acid ligase activity), GO:0015631 (tubulin binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0065008 (regulation of biological quality), GO:0006807 (nitrogen compound metabolic process), GO:0050794 (regulation of cellular process), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0007017 (microtubule-based process), GO:0044238 (primary metabolic process), GO:0040012 (regulation of locomotion), GO:0048522 (positive regulation of cellular process), GO:1901564 (organonitrogen compound metabolic process), GO:2000145 (regulation of cell motility), GO:0019538 (protein metabolic process), GO:0016043 (cellular component organization), GO:0000226 (microtubule cytoskeleton organization), GO:0032886 (regulation of microtubule-based process), GO:0043170 (macromolecule metabolic process), GO:0036211 (protein modification process), GO:1902019 (regulation of cilium-dependent cell motility), GO:0043412 (macromolecule modification), GO:0051013 (microtubule severing), GO:0060632 (regulation of microtubule-based movement), GO:0006996 (organelle organization), GO:0001578 (microtubule bundle formation), GO:0007010 (cytoskeleton organization), GO:0003352 (regulation of cilium movement), GO:0060295 (regulation of cilium movement involved in cell motility), GO:0018193 (peptidyl-amino acid modification), GO:0003356 (regulation of cilium beat frequency), GO:0018200 (peptidyl-glutamic acid modification), GO:0018095 (protein polyglutamylation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005815 (microtubule organizing center), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0042995 (cell projection), GO:0036064 (ciliary basal body), GO:0120025 (plasma membrane bounded cell projection), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0005929 (cilium), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005856 (cytoskeleton), GO:0097730 (non-motile cilium), GO:0097731 (9+0 non-motile cilium), GO:0015630 (microtubule cytoskeleton)