Protein: Phospholipase A and acyltransferase 3 (EC 2.3.1.-) (EC 3.1.1.32) (EC 3.1.1.4) (Group XVI phospholipase A2) (H-rev 107 protein homolog) (HRAS-like suppressor 3) (HRSL3) (Rat LRAT-like protein-3) (RLP-3) (P53817)
Organism: Rattus norvegicus (Rat)
Function: Exhibits both phospholipase A1/2 and acyltransferase activities. Shows phospholipase A1 (PLA1) and A2 (PLA2), ccatalyzing the calcium-independent release of fatty acids from the sn-1 or sn-2 position of glycerophospholipids. For most substrates, PLA1 activity is much higher than PLA2 activity (By similarity). Shows O-acyltransferase activity,catalyzing the transfer of a fatty acyl group from glycerophospholipid to the hydroxyl group of lysophospholipid (By similarity). Shows N-acyltransferase activity, catalyzing the calcium-independent transfer of a fatty acyl group at the sn-1 position of phosphatidylcholine (PC) and other glycerophospholipids to the primary amine of phosphatidylethanolamine (PE), forming N-acylphosphatidylethanolamine (NAPE), which serves as precursor for N-acylethanolamines (NAEs). Exhibits high N-acyltransferase activity and low phospholipase A1/2 activity (By similarity). Required for complete organelle rupture and degradation that occur during eye lens terminal differentiation, when fiber cells that compose the lens degrade all membrane-bound organelles in order to provide lens with transparency to allow the passage of light. Organelle membrane degradation is probably catalyzed by the phospholipase activity (By similarity).
Subcellular location: Cell membrane {ECO:0000269|PubMed:8290259}; Single-pass membrane protein {ECO:0000255}. Cytoplasm {ECO:0000269|PubMed:8290259}. Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q8R3U1}. Cytoplasm, perinuclear region {ECO:0000269|PubMed:8290259}. Peroxisome membrane {ECO:0000250|UniProtKB:Q8R3U1}. Mitochondrion membrane {ECO:0000250|UniProtKB:Q8R3U1}; Single-pass membrane protein {ECO:0000305}. Nucleus envelope {ECO:0000250|UniProtKB:Q8R3U1}. Lysosome membrane {ECO:0000250|UniProtKB:Q8R3U1}; Single-pass membrane protein {ECO:0000305}. Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q8R3U1}; Single-pass membrane protein {ECO:0000305}. Note=During eye lens differentiation, recruited from the cytosol to various organelles, including mitochondria, endoplasmic reticulum, nuclear envelope and lysosomes, immediately before organelle degradation. This translocation is triggered by organelle membrane damage and requires the C-terminal transmembrane domain. {ECO:0000250|UniProtKB:Q8R3U1}.
InterPro domains:
- IPR051496: H-rev107 Phospholipase/Acyltransferase (family) [4-158]
- IPR007053: LRAT domain (domain) [5-127]
Sequence:
MPIPEPKPGDLIEIFRPMYSHWAIYVGDGYVIHLAPPSEIPGAGAASIMSALTDKAIVKKELLRDVAGKDKYQVNNKHDKEYTPLPLNKIIQRAEELVGQEVLYRLTSENCEHFVNELRYGVPRSDQVRDAVKVATVTGVGLAALGLIGVMLSRNKKQKQ
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0016788 (hydrolase activity, acting on ester bonds), GO:0016298 (lipase activity), GO:0052689 (carboxylic ester hydrolase activity), GO:0008970 (phospholipase A1 activity), GO:0004620 (phospholipase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0050793 (regulation of developmental process), GO:0044237 (cellular metabolic process), GO:0051240 (positive regulation of multicellular organismal process), GO:0050794 (regulation of cellular process), GO:0065009 (regulation of molecular function), GO:0051239 (regulation of multicellular organismal process), GO:0071704 (organic substance metabolic process), GO:0051094 (positive regulation of developmental process), GO:0048523 (negative regulation of cellular process), GO:0044238 (primary metabolic process), GO:0048522 (positive regulation of cellular process), GO:0045597 (positive regulation of cell differentiation), GO:0050790 (regulation of catalytic activity), GO:0045682 (regulation of epidermis development), GO:0044093 (positive regulation of molecular function), GO:2000026 (regulation of multicellular organismal development), GO:0006629 (lipid metabolic process), GO:0042127 (regulation of cell population proliferation), GO:0044255 (cellular lipid metabolic process), GO:0045595 (regulation of cell differentiation), GO:0043603 (amide metabolic process), GO:0051962 (positive regulation of nervous system development), GO:0008284 (positive regulation of cell population proliferation), GO:0046486 (glycerolipid metabolic process), GO:0045604 (regulation of epidermal cell differentiation), GO:0043069 (negative regulation of programmed cell death), GO:0043067 (regulation of programmed cell death), GO:2000178 (negative regulation of neural precursor cell proliferation), GO:0045606 (positive regulation of epidermal cell differentiation), GO:0030858 (positive regulation of epithelial cell differentiation), GO:0050769 (positive regulation of neurogenesis), GO:0043085 (positive regulation of catalytic activity), GO:0030856 (regulation of epithelial cell differentiation), GO:0045604 (regulation of epidermal cell differentiation), GO:0006650 (glycerophospholipid metabolic process), GO:0051347 (positive regulation of transferase activity), GO:0014015 (positive regulation of gliogenesis), GO:0050767 (regulation of neurogenesis), GO:0051345 (positive regulation of hydrolase activity), GO:0043066 (negative regulation of apoptotic process), GO:0045616 (regulation of keratinocyte differentiation), GO:0033674 (positive regulation of kinase activity), GO:0014013 (regulation of gliogenesis)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0031975 (envelope), GO:0005829 (cytosol), GO:0048471 (perinuclear region of cytoplasm), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0005773 (vacuole), GO:0031967 (organelle envelope), GO:0042579 (microbody), GO:0005739 (mitochondrion), GO:0005783 (endoplasmic reticulum), GO:0005635 (nuclear envelope), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0000323 (lytic vacuole), GO:0043231 (intracellular membrane-bounded organelle), GO:0005777 (peroxisome), GO:0005764 (lysosome), GO:0005634 (nucleus)