lokahq/bioreason-rl huggingface dataset:
Protein: ER membrane protein complex subunit 6 (Transmembrane protein 93) (Q9VBZ8)
Organism: Drosophila melanogaster (Fruit fly)
Function: Not known
Subcellular location: Endoplasmic reticulum membrane {ECO:0000256|ARBA:ARBA00004477}; Multi-pass membrane protein {ECO:0000256|ARBA:ARBA00004477}.
InterPro domains:
- IPR008504: ER membrane protein complex subunit 6 (family) [5-113]
- IPR029008: ER membrane protein complex subunit 6-like (family) [29-107]
Sequence:
MNRVKTTQSKTGEIIAYSEGAIRNNISAVEYCRTSMAAISGCAAGILGLSGTLGFLFYFLSVFVLWILVLVKSGTQWRKFFINRRNLLTNQFMGGLCTYVLFWTFLYGMVHVY
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0140104 (molecular carrier activity), GO:0140597 (protein carrier chaperone), GO:0032977 (membrane insertase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0009987 (cellular process), GO:0051179 (localization), GO:0051234 (establishment of localization), GO:0051641 (cellular localization), GO:0071840 (cellular component organization or biogenesis), GO:0033036 (macromolecule localization), GO:0070727 (cellular macromolecule localization), GO:0016043 (cellular component organization), GO:0051668 (localization within membrane), GO:0045184 (establishment of protein localization), GO:0008104 (protein localization), GO:0006996 (organelle organization), GO:0010256 (endomembrane system organization), GO:0090150 (establishment of protein localization to membrane), GO:0061024 (membrane organization), GO:0072657 (protein localization to membrane), GO:0051205 (protein insertion into membrane), GO:0033365 (protein localization to organelle), GO:0007029 (endoplasmic reticulum organization), GO:0045048 (protein insertion into ER membrane), GO:0071816 (tail-anchored membrane protein insertion into ER membrane)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0140534 (endoplasmic reticulum protein-containing complex), GO:0016020 (membrane), GO:0098796 (membrane protein complex), GO:0031984 (organelle subcompartment), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0031090 (organelle membrane), GO:0043229 (intracellular organelle), GO:0098827 (endoplasmic reticulum subcompartment), GO:0042175 (nuclear outer membrane-endoplasmic reticulum membrane network), GO:0005783 (endoplasmic reticulum), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0098588 (bounding membrane of organelle), GO:0005789 (endoplasmic reticulum membrane)
Protein: N-terminal Xaa-Pro-Lys N-methyltransferase 2 (EC 2.1.1.299) (Alpha N-terminal protein methyltransferase 1B) (Methyltransferase-like protein 11B) (X-Pro-Lys N-terminal protein methyltransferase 1B) (NTM1B) (Q5VVY1)
Organism: Homo sapiens (Human)
Function: Alpha N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes monomethylation of exposed alpha-amino group of Ala or Ser residue in the [Ala/Ser]-Pro-Lys motif and Pro in the Pro-Pro-Lys motif. Predominantly functions as a mono-methyltransferase but is also able to di-/tri-methylate the GPKRIA peptide and di-methylate the PPKRIA peptide (in vitro). May activate NTMT1 by priming its substrates for trimethylation.
Subcellular location: Nucleus {ECO:0000269|PubMed:24090352}.
InterPro domains:
- IPR008576: Alpha-N-methyltransferase NTM1 (family) [60-278]
- IPR029063: S-adenosyl-L-methionine-dependent methyltransferase superfamily (homologous_superfamily) [57-278]
Sequence:
MAHRGAHFAFRSRWQKTDDELCRHSMSFILHKAIRNDFFQSYLYLLEKIPLVKLYALTSQVINGEMQFYARAKLFYQEVPATEEGMMGNFIELSSPDIQASQKFLRKFVGGPGRAGTDCALDCGSGIGRVSKHVLLPVFNSVELVDMMESFLLEAQNYLQVKGDKVESYHCYSLQEFTPPFRRYDVIWIQWVSGHLTDKDLLAFLSRCRDGLKENGIIILKDNVAREGCILDLSDSSVTRDMDILRSLIRKSGLVVLGQEKQDGFPEQCIPVWMFALHSDRHS
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0005515 (protein binding), GO:0016741 (transferase activity, transferring one-carbon groups), GO:0008276 (protein methyltransferase activity), GO:0042054 (histone methyltransferase activity), GO:0008168 (methyltransferase activity), GO:0008757 (S-adenosylmethionine-dependent methyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0032259 (methylation), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0019538 (protein metabolic process), GO:0043414 (macromolecule methylation), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0036211 (protein modification process), GO:0043412 (macromolecule modification), GO:0006479 (protein methylation), GO:0008213 (protein alkylation), GO:0031365 (N-terminal protein amino acid modification), GO:0018193 (peptidyl-amino acid modification), GO:0018208 (peptidyl-proline modification)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)
Protein: Meiotically up-regulated gene 82 protein (Q9HDZ3)
Organism: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Function: Has a role in meiosis.
Subcellular location: Mitochondrion {ECO:0000269|PubMed:16823372}.
InterPro domains:
- IPR000352: Peptide chain release factor class I (domain) [46-176]
- IPR045853: Peptide chain release factor class I superfamily (homologous_superfamily) [44-133]
- IPR052104: Mitochondrial Release Factor mL62 (family) [46-182]
Sequence:
MFANFRNCFKIKNSRLIYDNINKCLLTKEETNQLLKFIHLKWKPAKDQVQISFSRSSGPGGQNVNKLNTKVIVNLPFKQLESCIPMFLINHFKTCEMLRNYRIQNGIKIYSQKTRSQHKNIEDALNKISDLLNKSAETLYVPDTPPEKIARISILKKESNEKRLSEKKYKQKKKTQRRITMD
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0016740 (transferase activity), GO:0140098 (catalytic activity, acting on RNA), GO:0016765 (transferase activity, transferring alkyl or aryl (other than methyl) groups), GO:0140101 (catalytic activity, acting on a tRNA)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044238 (primary metabolic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0044249 (cellular biosynthetic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043603 (amide metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0019538 (protein metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0006412 (translation), GO:0009059 (macromolecule biosynthetic process), GO:0043604 (amide biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0034645 (cellular macromolecule biosynthetic process), GO:0006518 (peptide metabolic process), GO:0010467 (gene expression), GO:0140053 (mitochondrial gene expression), GO:0043043 (peptide biosynthetic process), GO:0032543 (mitochondrial translation)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0005739 (mitochondrion), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)
Protein: RanBP2-type domain-containing protein (Q38B21)
Organism: Trypanosoma brucei brucei (strain 927/4 GUTat10.1)
Function: Not known
InterPro domains:
- IPR036443: Zinc finger, RanBP2-type superfamily (homologous_superfamily) [69-530]
- IPR001876: Zinc finger, RanBP2-type (domain) [68-531]
Sequence:
MYLHSMKRYIRAPLAPLLNVGRSNSSCGGAVCFCAPCAGRTAYCPAYATQVAAFCMPTAALLQSWAVRTNRTWMCVKEECRHVNAPGATHCESCGEVKPNLKGWLCVECSTRNHKGVKKCHKCAAPAEKSSEFWMCAACEKNNRIDDLDDNSCCGFCGYDMAPMTMTEAEALRIQQERSERLRETQERFDALSAKDADEQFGDEMAGASELPAALKQGVPPAPRTMFDIPEVKPFAPTPQGTKHSRILRKPRPASSPGAPSGPPGFDWMCREPSCAVINSGDEECCTGCGKHIEPTEWECCHCGAVNHMTRARCFNCRVTISISWVCSDCRTATCIYERSCRACNKARPPTEPKEARDVQPPSQNAGRAGFSANRRRMPFKQDWNCEECQGLNFASRTSCYQCGAARSTADASYNGGASGGDGGFDGGAGLSVSHNNWFCRHCQASNFRTRASCWQCGRASSESGATTWSEDDSAPHFEKEGFQQTSDDNVAEGQVNVWNKKTDDWTCGKCFSKNFKNRQECHKCGATKTVAVAPRRAFVRKPVKI
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0003676 (nucleic acid binding), GO:0003723 (RNA binding), GO:0003729 (mRNA binding)
Biological Process (BP): GO:0008150 (biological process), GO:0050789 (regulation of biological process), GO:0065007 (biological regulation), GO:0019222 (regulation of metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0010468 (regulation of gene expression), GO:0010608 (post-transcriptional regulation of gene expression)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0042995 (cell projection), GO:0005737 (cytoplasm), GO:0031974 (membrane-enclosed lumen), GO:0043233 (organelle lumen), GO:0120025 (plasma membrane bounded cell projection), GO:0043229 (intracellular organelle), GO:0099568 (cytoplasmic region), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005929 (cilium), GO:0032838 (plasma membrane bounded cell projection cytoplasm), GO:0070013 (intracellular organelle lumen), GO:0031981 (nuclear lumen), GO:0097014 (ciliary plasm), GO:0005634 (nucleus)
Protein: D-galactonate dehydratase family member Cbei_4837 (A6M2W4)
Organism: Clostridium beijerinckii (strain ATCC 51743 / NCIMB 8052) (Clostridium acetobutylicum)
Function: Has no detectable activity with D-mannonate and with a panel of 70 other acid sugars (in vitro), in spite of the conservation of the residues that are expected to be important for catalytic activity and cofactor binding. May have evolved a divergent function.
InterPro domains:
- IPR029065: Enolase C-terminal domain-like (domain) [181-372]
- IPR018110: Mandelate racemase/muconate lactonizing enzyme, conserved site (conserved_site) [89-114]
- IPR013341: Mandelate racemase/muconate lactonizing enzyme, N-terminal domain (domain) [18-114]
- IPR013342: Mandelate racemase/muconate lactonizing enzyme, C-terminal (domain) [132-252]
- IPR029017: Enolase-like, N-terminal (homologous_superfamily) [11-395]
- IPR034589: D-mannonate dehydratase-like (family) [5-398]
- IPR034593: D-galactonate dehydratase DgoD-like (family) [18-380]
- IPR036849: Enolase-like, C-terminal domain superfamily (homologous_superfamily) [102-380]
Sequence:
MEPTIITDVLCYITKPDRHNLVVVKVETNKGIYGLGCATFQQRPKAVSLVVSEYLKPILIGRDANNIEDLWQMMMVNSYWRNGPILNNAISGVDMALWDIKGKLANMPLYQLFGGKSRDAIAAYTHAVADNLEDLYTEIDEIRKKGYQHIRCQLGFYGGNSSEFHTTDNPTQGSYFDQDEYMRTTVSMFSSLREKYGYKFHILHDVHERLFPNQAVQFAKDVEKYKPYFIEDILPPDQNEWLGQIRSQTSTPLATGELFNNPMEWKSLIANRQVDFIRCHVSQIGGITPALKLGSLCAAFGVRIAWHTPSDITPIGVAVNIHLNINLHNAAIQENIEINDNTRCVFSGIPEAKNGFFYPIESPGIGVDIDENEIIKYPVEYRPHEWTQSRIPDGTIVTP
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0043167 (ion binding), GO:0043169 (cation binding), GO:0046872 (metal ion binding), GO:0000287 (magnesium ion binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009056 (catabolic process), GO:0044238 (primary metabolic process), GO:0071704 (organic substance metabolic process), GO:1901575 (organic substance catabolic process), GO:0005975 (carbohydrate metabolic process), GO:0016052 (carbohydrate catabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0071944 (cell periphery), GO:0030312 (external encapsulating structure), GO:0005618 (cell wall), GO:0009274 (peptidoglycan-based cell wall)
Protein: Holocytochrome c-type synthase (EC 4.4.1.17) (A0A1D8PN32)
Organism: Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Function: Not known
Subcellular location: Mitochondrion inner membrane {ECO:0000256|ARBA:ARBA00004273, ECO:0000256|RuleBase:RU363130}.
InterPro domains:
- IPR000511: Holocytochrome c/c1 synthase (family) [1-259]
Sequence:
MGWFWADKPSQDTVKATSFFTTPSACPIDHSKLASSSPTCPVKLNNDNDEVLNPLNNMPMAISSERAPGQRIKLSTERTISTIPRGESEDQGLWEYPSPQQMLNAMLSKGKGDGVPEDAVESMVEVHNFLNEGAWQQILTWEDQYTQQTKVEPRLKKFTGRPHDLSPKARMYLWLGQLFPETFNTIPPFDRHDWTVLRSCGRNQGWKEVRYVIDYYGAPDDEETGMPAFMLDTRPALDNLTNARDRFTHWAYPLWKKAMGEVRD
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016829 (lyase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0016846 (carbon-sulfur lyase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0071840 (cellular component organization or biogenesis), GO:0009058 (biosynthetic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0016043 (cellular component organization), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0044085 (cellular component biogenesis), GO:0019538 (protein metabolic process), GO:0043170 (macromolecule metabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0009059 (macromolecule biosynthetic process), GO:0043933 (protein-containing complex organization), GO:0006996 (organelle organization), GO:0022607 (cellular component assembly), GO:0065003 (protein-containing complex assembly), GO:0007005 (mitochondrion organization), GO:0033108 (mitochondrial respiratory chain complex assembly), GO:0017004 (cytochrome complex assembly), GO:0008535 (respiratory chain complex IV assembly), GO:0033617 (mitochondrial cytochrome c oxidase assembly)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0071944 (cell periphery), GO:0030312 (external encapsulating structure), GO:0005618 (cell wall), GO:0009277 (fungal-type cell wall), GO:0030446 (hyphal cell wall)
Protein: Prostate and testis expressed protein 14 (Secreted seminal-vesicle Ly-6 protein 1) (SSLP-1) (Q3UN54)
Organism: Mus musculus (Mouse)
Function: Not known
Subcellular location: Secreted {ECO:0000269|PubMed:16940290}.
InterPro domains:
- IPR016054: Ly-6 antigen/uPA receptor-like (domain) [20-99]
Sequence:
MEKYLLLLLLGIFLRVGFLQALTCVSCGRLNSSGICETAETSCEATNNRKCALRLLYKDGKFQYGFQGCLGTCFNYTKTNNNMVKEHKCCDHQNLCNKP
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0098772 (molecular function regulator activity), GO:0030545 (signaling receptor regulator activity), GO:0099602 (neurotransmitter receptor regulator activity), GO:0030548 (acetylcholine receptor regulator activity)
Biological Process (BP): GO:0008150 (biological process), GO:0050896 (response to stimulus), GO:0050789 (regulation of biological process), GO:0065007 (biological regulation), GO:0023051 (regulation of signaling), GO:0006950 (response to stress), GO:0050794 (regulation of cellular process), GO:0009611 (response to wounding), GO:0010646 (regulation of cell communication), GO:0099177 (regulation of trans-synaptic signaling), GO:0050804 (modulation of chemical synaptic transmission)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005576 (extracellular region), GO:0005615 (extracellular space)
Protein: Replicative helicase loader DnaI (EC 3.6.4.-) (Primosomal protein DnaI) (P06567)
Organism: Bacillus subtilis (strain 168)
Function: Probably involved in DNA replication.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:10844689}. Note=Forms foci near both cell poles and in the middle of cells (PubMed:10844689). {ECO:0000269|PubMed:10844689}.
InterPro domains:
- IPR009928: Primosomal DnaI, N-terminal (domain) [1-98]
- IPR003593: AAA+ ATPase domain (domain) [160-284]
- IPR013317: Chromosomal replication initiator protein DnaA, ATPAse domain (domain) [163-271]
- IPR027417: P-loop containing nucleoside triphosphate hydrolase (homologous_superfamily) [108-302]
Sequence:
MEPIGRSLQGVTGRPDFQKRLEQMKEKVMKDQDVQAFLKENEEVIDQKMIEKSLNKLYEYIEQSKNCSYCSEDENCNNLLEGYHPKLVVNGRSIDIEYYECPVKRKLDQQKKQQSLMKSMYIQQDLLGATFQQVDISDPSRLAMFQHVTDFLKSYNETGKGKGLYLYGKFGVGKTFMLAAIANELAEKEYSSMIVYVPEFVRELKNSLQDQTLEEKLNMVKTTPVLMLDDIGAESMTSWVRDEVIGTVLQHRMSQQLPTFFSSNFSPDELKHHFTYSQRGEKEEVKAARLMERILYLAAPIRLDGENRRHP
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0009987 (cellular process), GO:0071840 (cellular component organization or biogenesis), GO:0016043 (cellular component organization), GO:0044085 (cellular component biogenesis), GO:0043933 (protein-containing complex organization), GO:0022607 (cellular component assembly), GO:0065003 (protein-containing complex assembly), GO:0051259 (protein complex oligomerization), GO:0051260 (protein homooligomerization)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0042597 (periplasmic space)
Protein: Flotillin-like protein 2 (D2XNQ9)
Organism: Medicago truncatula (Barrel medic) (Medicago tribuloides)
Function: May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles (By similarity). Required for early symbiotic events and nodules formation.
Subcellular location: Cell membrane {ECO:0000305}; Lipid-anchor {ECO:0000305}. Membrane, caveola {ECO:0000250}. Note=In puncta evenly distributed in the cell membrane of root hair cells and polarly localized in root epidermal cells. No changes of localization upon inoculation with bacteria. {ECO:0000269|PubMed:20018678}.
InterPro domains:
- IPR001107: Band 7 domain (domain) [7-186]
- IPR027705: Flotillin family (family) [3-477]
- IPR036013: Band 7/SPFH domain superfamily (homologous_superfamily) [35-175]
Sequence:
MKIYRVAKASEYLVITGIFIKDIKLKKKAWIFPGQSCTVLDLSPVNYTFEVQAMSAEKLPFVLPAVFTIGPRVDDQESLLKYAKLISPHDRHSNHVNELVQGIIEGETRVLAASMTMEEVFRGTKQFKQEVFDKVQLELNQFGLLIYNANVKQLVDVPGHEYFSYLGQKTQMEAKNQARVDVSEAKMKGEIGSKLREGQTLQNAAKIDAETKVIAMQRAGEGEKEGIKVRTEVKVFENQREAEVAQANSELAKKKAAWTKAAQVAEVEAKKAVALREAELQGEVERMNALTTTEKLKADLLSKASVQYETKVQEANWELYKKQKETEAILYEKKAEAEAQKASADATFYASKQAAEAELYAKKKEAEGIVTLGQAQGAYVSTLLNALGNDYTAVRDYLMINGDMFQEIAKINAEAIRGLEPKISIWTNGGDNSGGITDGAMGMKEVAGVYKMLPPLFKTVHEQTGMLPPAWMGALSEKSS
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0032502 (developmental process), GO:0032501 (multicellular organismal process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0099402 (plant organ development), GO:0048731 (system development), GO:0048364 (root development), GO:0022622 (root system development)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0045177 (apical part of cell), GO:0071944 (cell periphery), GO:0016020 (membrane), GO:0098590 (plasma membrane region), GO:0005886 (plasma membrane), GO:0016324 (apical plasma membrane)
Protein: Secreted effector protein SseJ (EC 3.-.-.-) (Salmonella-translocated effector J) (STE J) (Q9FD10)
Organism: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Function: Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. This protein is required for endosomal tubulation and negatively regulates the formation of Salmonella-induced filaments (Sifs) in epithelial cells. Has both deacylase and esterification activities in vitro, but esterification is probably the dominant activity in host cells. Significantly contributes to cholesterol esterification, which reduces cellular cholesterol in cells and abrogates the ability of SifA to associate with cholesterol and LAMP-1 vesicles.
Subcellular location: Secreted. Host cytoplasm. Note=Secreted via type III secretion system 2 (SPI-2 T3SS), and delivered into the host cytoplasm. Localizes to the Salmonella-containing vacuole (SCV) at early time points following invasion and subsequently traffics away from the SCV along Salmonella-induced filaments (Sifs) in epithelial cells.
InterPro domains:
- IPR001087: GDSL lipase/esterase (family) [145-394]
- IPR010637: Sif (family) [1-108]
- IPR036514: SGNH hydrolase superfamily (homologous_superfamily) [137-405]
Sequence:
MPLSVGQGYFTSSISSEKFNAIKESARLPELSLWEKIKAYFFTTHHAEALECIFNLYHHQELNLTPVQVRGAYIKLRALASQGCKEQFIIESQEHADKLIIKDDNGENILSIEVECHPEAFGLAKEINKSHPKPKNISLGDITRLVFFGDSLSDSLGRMFEKTHHILPSYGQYFGGRFTNGFTWTEFLSSPHFLGKEMLNFAEGGSTSASYSCFNCIGDFVSNTDRQVASYTPSHQDLAIFLLGANDYMTLHKDNVIMVVEQQIDDIEKIISGGVNNVLVMGIPDLSLTPYGKHSDEKRKLKDESIAHNALLKTNVEELKEKYPQHKICYYETADAFKVIMEAASNIGYDTENPYTHHGYVHVPGAKDPQLDICPQYVFNDLVHPTQEVHHCFAIMLESFIAHHYSTE
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0044419 (biological process involved in interspecies interaction between organisms), GO:0044403 (biological process involved in symbiotic interaction), GO:0035821 (modulation of process of another organism), GO:0051701 (biological process involved in interaction with host), GO:0044003 (modulation by symbiont of host process), GO:0052111 (modification by symbiont of host structure), GO:0052043 (modification by symbiont of host cellular component)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0018995 (host cellular component), GO:0033643 (host cell part), GO:0043657 (host cell), GO:0033644 (host cell membrane), GO:0033646 (host intracellular part), GO:0043656 (host intracellular region), GO:0033655 (host cell cytoplasm part), GO:0033647 (host intracellular organelle), GO:0020002 (host cell plasma membrane), GO:0030430 (host cell cytoplasm), GO:0033648 (host intracellular membrane-bounded organelle), GO:0044165 (host cell endoplasmic reticulum)