Protein: D-rhamnosyltransferase WbpZ (EC 2.4.1.-) (GDP-D-Man:GlcNAc-diphosphate-lipid alpha-1,3-D-mannosyltransferase) (EC 2.4.1.348) (GDP-D-Man:GlcNAc/GalNAc-diphosphate-lipid alpha-1,3-D-mannosyltransferase) (GDP-D-rhamnose:GlcNAc/GalNAc-diphosphate-lipid alpha-1,3-D-rhamnosyltransferase) (Q9HTC0)
Organism: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Function: Non-processive alpha-1,3-D-rhamnosyltransferase. Catalyzes the transfer of one D-rhamnose (D-Rha) residue from donor substrate GDP-D-Rha in alpha-1-3 linkage to both GlcNAc- and GalNAc-diphosphate-lipid acceptor substrates. Is also able to transfer D-mannose (D-Man) to these acceptors at a lower level. Nucleotide sugars GDP-D-Rha, GDP-Fuc, UDP-Gal, UDP-GalNAc, UDP-GlcNAc and CMP-sialic acid cannot act as donor substrates. Only compounds with a diphosphate as the aglycone group can act as acceptor substrates. No activity is detected with compounds containing a diphosphate mimic. Fluorescent undecyl-anthracenyl group-containing compounds, such as GlcNAc-PO(3)-PO(3)-AnthrU and GalNAc-PO(3)-PO(3)-AnthrU, are also good acceptor substrates. Involved in the biosynthesis of the common polysaccharide antigen (CPA), also called A band, which is one of the two major cell surface O-antigens of the P.aeruginosa lipopolysaccharide. Involved in susceptibility to antibiotic colistin.
Subcellular location: Cytoplasm {ECO:0000305|PubMed:23664878}.
InterPro domains:
- IPR028098: Glycosyltransferase subfamily 4-like, N-terminal domain (domain) [15-172]
- IPR001296: Glycosyl transferase, family 1 (domain) [188-345]
Sequence:
MRVLHFYKTYLSETVGGIEQVIFQLCESSGSWGIDNHVLTLSSDPHPPVVPFGGHVVHRARLDLQLASTGFSLSVFKQFRELAAEADVVNYHFPWPFMDLVHFLTGMNKPSVVTYHSDIIRQRVLLKLYRPLMSRFLHSVDRIVAASPNYFSTSDVLRQYREKTRVITYGLDKDGYPKPATQRLEHWREKLGPRFFLFVGVMRYYKGLHILLDALQGTDYPVVIVGAGPLQAELYAQAAALGLRNVHFLGRVDDEDKVALLQLSYAMVFPSHLRSEAFGISLLEGAMYGKPMISSEIGTGTSYINIHGETGLVVPPSQPAAFRQAMRWLWEHPQQAEEMGRNAEARYRQLFTAEEMGRRWSELYRELLEEKASSRYVKAAR
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016757 (glycosyltransferase activity), GO:0016758 (hexosyltransferase activity), GO:0000030 (mannosyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006629 (lipid metabolic process), GO:0044255 (cellular lipid metabolic process), GO:0044249 (cellular biosynthetic process), GO:0005975 (carbohydrate metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:1901135 (carbohydrate derivative metabolic process), GO:0044262 (cellular carbohydrate metabolic process), GO:0009059 (macromolecule biosynthetic process), GO:0016051 (carbohydrate biosynthetic process), GO:0005976 (polysaccharide metabolic process), GO:0034637 (cellular carbohydrate biosynthetic process), GO:1903509 (liposaccharide metabolic process), GO:0034645 (cellular macromolecule biosynthetic process), GO:1901137 (carbohydrate derivative biosynthetic process), GO:0008610 (lipid biosynthetic process), GO:0008653 (lipopolysaccharide metabolic process), GO:0044264 (cellular polysaccharide metabolic process), GO:0046402 (O antigen metabolic process), GO:0009103 (lipopolysaccharide biosynthetic process), GO:0033692 (cellular polysaccharide biosynthetic process), GO:0000271 (polysaccharide biosynthetic process), GO:0009243 (O antigen biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0016020 (membrane)