lokahq/bioreason-rl huggingface dataset:
Protein: Ribosomal RNA small subunit methyltransferase H (EC 2.1.1.199) (16S rRNA m(4)C1402 methyltransferase) (rRNA (cytosine-N(4)-)-methyltransferase RsmH) (P60390)
Organism: Escherichia coli (strain K12)
Function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA. In vitro, active on the assembled 30S subunit, but not naked 16S rRNA or 70S ribosomes.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:10572301}.
InterPro domains:
- IPR002903: Ribosomal RNA small subunit methyltransferase H (family) [1-312]
- IPR029063: S-adenosyl-L-methionine-dependent methyltransferase superfamily (homologous_superfamily) [7-310]
- IPR023397: S-adenosyl-L-methionine-dependent methyltransferase, MraW, recognition domain superfamily (homologous_superfamily) [112-214]
Sequence:
MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQLGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDLGVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERFAKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEEIEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKLGGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140640 (catalytic activity, acting on a nucleic acid), GO:0005515 (protein binding), GO:0140098 (catalytic activity, acting on RNA), GO:0016741 (transferase activity, transferring one-carbon groups), GO:0140102 (catalytic activity, acting on a rRNA), GO:0008173 (RNA methyltransferase activity), GO:0008168 (methyltransferase activity), GO:0008649 (rRNA methyltransferase activity), GO:0008757 (S-adenosylmethionine-dependent methyltransferase activity), GO:0008170 (N-methyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0032259 (methylation), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0043414 (macromolecule methylation), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0043412 (macromolecule modification), GO:0090304 (nucleic acid metabolic process), GO:0001510 (RNA methylation), GO:0010467 (gene expression), GO:0030488 (tRNA methylation), GO:0016070 (RNA metabolic process), GO:0006396 (RNA processing), GO:0009451 (RNA modification), GO:0034470 (ncRNA processing), GO:0006400 (tRNA modification), GO:0034660 (ncRNA metabolic process), GO:0006399 (tRNA metabolic process), GO:0008033 (tRNA processing)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)
Protein: Large ribosomal subunit protein uL1z (60S ribosomal protein L10a-1) (Q8VZB9)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Not known
InterPro domains:
- IPR023673: Large ribosomal subunit protein uL1, conserved site (conserved_site) [116-135]
- IPR023674: Ribosomal protein uL1-like (homologous_superfamily) [20-216]
- IPR028364: Ribosomal protein uL1/ribosomal biogenesis protein (family) [9-215]
- IPR050257: Large ribosomal subunit protein eL8/uL1-like (family) [11-209]
- IPR016095: Large ribosomal subunit protein uL1, 3-layer alpha/beta-sandwich domain (homologous_superfamily) [65-154]
- IPR002143: Large ribosomal subunit protein uL1 (family) [1-216]
Sequence:
MSKLQSEAVREAITTITGKSEAKKRNFVETIELQIGLKNYDPQKDKRFSGSVKLPHIPRPKMKICMLGDAQHVEEAEKMGLENMDVESLKKLNKNKKLVKKLAKKYHAFLASESVIKQIPRLLGPGLNKAGKFPTLVSHQESLESKVNETKATVKFQLKKVLCMGVAVGNLSMEEKQIFQNVQMSVNFLVSLLKKNWQNVRCLYLKSTMGPPQRIF
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005198 (structural molecule activity), GO:0003735 (structural constituent of ribosome)
Biological Process (BP): GO:0008150 (biological process), GO:0032502 (developmental process), GO:0032501 (multicellular organismal process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0009791 (post-embryonic development), GO:0099402 (plant organ development), GO:0048731 (system development), GO:0090696 (post-embryonic plant organ development)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:1990904 (ribonucleoprotein complex), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005829 (cytosol), GO:0005737 (cytoplasm), GO:0044391 (ribosomal subunit), GO:0042579 (microbody), GO:0005844 (polysome), GO:0043229 (intracellular organelle), GO:0022626 (cytosolic ribosome), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0042788 (polysomal ribosome), GO:0043231 (intracellular membrane-bounded organelle), GO:0005777 (peroxisome), GO:0015934 (large ribosomal subunit), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0022625 (cytosolic large ribosomal subunit), GO:0005840 (ribosome)
Protein: Tat pathway signal protein (O25542)
Organism: Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Function: Not known
InterPro domains:
- IPR019546: Twin-arginine translocation pathway, signal sequence, bacterial/archaeal (conserved_site) [3-21]
Sequence:
MKRRDFIKTTTLGATGAVLGAQILQAEESKGSVAKYKIEAQYSIDFDSAEHTSLFIPMPSVVASNVHLQGNHASYKSMLNFGVPYLQVDFLKSTQKKQVHLSYEIASYQLNERLFETSDFVAMGRYERDDASVANIANQLKGTTPKESVRNFYAFIKHEMPKRQKALEGKENLPKRESLPWFATISKESMFVSLCHACGIKSAEVQGLKLGQNSVVKNAPRVEVYLKDSFLAFDFQNNHKEVFIPLNRHKDMQLDSALLATFGDAFALVDGRDLGNYESKLFEKRVSYTIV
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0044255 (cellular lipid metabolic process), GO:0044249 (cellular biosynthetic process), GO:0005975 (carbohydrate metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0006629 (lipid metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0044262 (cellular carbohydrate metabolic process), GO:1901135 (carbohydrate derivative metabolic process), GO:0009059 (macromolecule biosynthetic process), GO:1903509 (liposaccharide metabolic process), GO:0034645 (cellular macromolecule biosynthetic process), GO:1901137 (carbohydrate derivative biosynthetic process), GO:0008610 (lipid biosynthetic process), GO:0008653 (lipopolysaccharide metabolic process), GO:0044264 (cellular polysaccharide metabolic process), GO:0016051 (carbohydrate biosynthetic process), GO:0005976 (polysaccharide metabolic process), GO:0034637 (cellular carbohydrate biosynthetic process), GO:0000271 (polysaccharide biosynthetic process), GO:0009103 (lipopolysaccharide biosynthetic process), GO:0033692 (cellular polysaccharide biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0042597 (periplasmic space)
Protein: Neuralized E3 ubiquitin protein ligase 1 (A0A096MJM7)
Organism: Rattus norvegicus (Rat)
Function: Not known
InterPro domains:
- IPR006573: Neuralized homology repeat (NHR) domain (domain) [43-431]
- IPR037962: Neuralized (family) [42-556]
- IPR013083: Zinc finger, RING/FYVE/PHD-type (homologous_superfamily) [498-556]
- IPR043136: B30.2/SPRY domain superfamily (homologous_superfamily) [48-430]
- IPR001841: Zinc finger, RING-type (domain) [504-544]
Sequence:
MGGQITRNTLHDSIGSSFPVPSHRCHHKQKHCPPALSGGGLPATPLLFHPHTKGSQILMDLSHKAVKRQASFCNAITFSNRPVLIYEQVRLKITKKQCCWSGALRLGFTSKDPSRIHPDSLPKYACPDLVSQSGFWAKALPEEFANEGNIIAFWVDKKGRVFYRINESAAMLFFSGVRTADPLWALVDVYGLTRGVQLLDSELVLPDCLRPRSFTALRRPSLRCEADEARLSVSLCDLNVPGADGEDGAPPAGCPIPQNSLNSQHSRALPAQLDGDLRFHALRAGAHVRILDEQTVARLEHGRDERALVFTSRPVRVAETIFIKVTRSGGARPGALSFGVTTCDPGTLRPADLPFSPEALVDRKEFWAVCRVPGPLHSGDILGLVVNADGELHLSHNGAPAGMQLCVDASQPLWMLFSLHGAITQVRILGSTIMAERGGPSLPCSPASTPTSPSALGSRLSDPLLSTCGSGPLGGSAGGTAPNSPVSLPESPVTPGLGQWSDECTICYEHAVDTVIYTCGHMCLCYSCGLRLKKALHACCPICRRPIKDIIKTYRSS
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0005515 (protein binding), GO:0019787 (ubiquitin-like protein transferase activity), GO:0004842 (ubiquitin-protein transferase activity), GO:0061659 (ubiquitin-like protein ligase activity), GO:0061630 (ubiquitin protein ligase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0032502 (developmental process), GO:0032501 (multicellular organismal process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0061061 (muscle structure development), GO:0009888 (tissue development), GO:0060322 (head development), GO:0048731 (system development), GO:0048513 (animal organ development), GO:0007420 (brain development), GO:0007417 (central nervous system development), GO:0007399 (nervous system development), GO:0007517 (muscle organ development), GO:0060537 (muscle tissue development), GO:0007519 (skeletal muscle tissue development), GO:0060538 (skeletal muscle organ development)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0043204 (perikaryon), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0042995 (cell projection), GO:0016020 (membrane), GO:0030054 (cell junction), GO:0098794 (postsynapse), GO:0044297 (cell body), GO:0036477 (somatodendritic compartment), GO:0005737 (cytoplasm), GO:0071944 (cell periphery), GO:0043025 (neuronal cell body), GO:0043197 (dendritic spine), GO:0120025 (plasma membrane bounded cell projection), GO:0097447 (dendritic tree), GO:0045202 (synapse), GO:0005886 (plasma membrane), GO:0099572 (postsynaptic specialization), GO:0043005 (neuron projection), GO:0098984 (neuron to neuron synapse), GO:0014069 (postsynaptic density), GO:0030425 (dendrite), GO:0097440 (apical dendrite), GO:0032279 (asymmetric synapse), GO:0044309 (neuron spine)
Protein: PANX2 protein (Q495U3)
Organism: Homo sapiens (Human)
Function: Not known
InterPro domains:
- IPR039099: Pannexin (family) [1-172]
Sequence:
MNLIILVNLIHLFIFRKSNFIFDKLHKVGIKTRRQWRRSQFCDINILAMFCNENRDHIKSLNRLDFITNESDLMYDNVVRQLLAALAQSNHDATPTVRDSGVQTVDPSANPAEPDGAAEPPVVKRPRKKMKWIPTSNPLPQPFKEPLAIMRVENSKAEKPKPARRKTATDTLIAPLLDRSAPDKKHARHFSLDVHPYILGTKKAKAEAVPAALPASRSQEGGFLSQAEDCGLGLAPAPIKDAPLPEKEIPYPTEPARAGLPSGGPFHVRSPPAAPAVAPLTPASLGKAEPLTILSRNATHPLLHINTLYEAREEEDGGPRLPQDVGDLIAIPAPQQILIATFDEPRTVVSTVEF
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0050789 (regulation of biological process), GO:0065007 (biological regulation), GO:0048518 (positive regulation of biological process), GO:0050794 (regulation of cellular process), GO:0048522 (positive regulation of cellular process), GO:0051128 (regulation of cellular component organization), GO:0044089 (positive regulation of cellular component biogenesis), GO:0051130 (positive regulation of cellular component organization), GO:0044087 (regulation of cellular component biogenesis), GO:0043254 (regulation of protein-containing complex assembly), GO:0031334 (positive regulation of protein-containing complex assembly), GO:1900225 (regulation of NLRP3 inflammasome complex assembly)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0043229 (intracellular organelle), GO:0043233 (organelle lumen), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0031981 (nuclear lumen), GO:0005634 (nucleus)
Protein: Dihydropyrimidinase-related protein 2 (DRP-2) (Neural-specific protein NSP60) (O02675)
Organism: Bos taurus (Bovine)
Function: Plays a role in neuronal development and polarity, as well as in axon growth and guidance, neuronal growth cone collapse and cell migration. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. May play a role in endocytosis (By similarity).
Subcellular location: Cytoplasm, cytosol {ECO:0000250}. Cytoplasm, cytoskeleton {ECO:0000250}. Membrane {ECO:0000250}. Note=Tightly but non-covalently associated with membranes. {ECO:0000250}.
InterPro domains:
- IPR050378: Metallo-dependent Hydrolases Superfamily (family) [11-502]
- IPR011059: Metal-dependent hydrolase, composite domain superfamily (homologous_superfamily) [15-485]
- IPR011778: Hydantoinase/dihydropyrimidinase (family) [17-471]
- IPR006680: Amidohydrolase-related (domain) [64-453]
- IPR032466: Metal-dependent hydrolase (homologous_superfamily) [68-399]
Sequence:
MSYQGKKNIPRITSDRLLIKGGKIVNDDQSFYADIYMEDGLIKQIGENLIVPGGVKTIEAHSRMVIPGGIDVHTRFQMPDQGMTSADDFFQGTKAALAGGTTMIIDHVVPEPGTSLLAAFDQWREWADSKSCCDYSLHVDITEWHKGVQEEMEALVKDHGVNSFLVYMAFKDRFQLTDSQIYEVLSVIRDIGAIAQVHAENGDIIAEEQQRILDLGITGPEGHVLSRPEEVEAEAVNRSITIANQTNCPLYITKVMSKSAAEVIAQARKKGTVVYGEPITASLGTDGSHYWSKNWAKAAAFVTSPPLSPDPTTPDFLNSLLSCGDLQVTGSAHCTFNTAQKAVGKDNFTLIPEGTNGTEERMSVIWDKAVVTGKMDENQFVAVTSTNAAKVFNLYPRKGRIAVGSDADLVIWDPDSVKTISAKTHNSSLEYNIFEGMECRGSPLVVISQGKIVLEDGTLHVTEGSGRYIPRKPFPDFVYKRIKARSRLAELRGVPRGLYDGPVCEVSVTPKTVTPASSAKTSPAKQQAPPVRNLHQSGFSLSGAQIDDNIPRRTTQRIVAPPGGRANITSLG
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:0006139 (nucleobase-containing compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0046483 (heterocycle metabolic process), GO:0090304 (nucleic acid metabolic process), GO:0010467 (gene expression), GO:0016070 (RNA metabolic process), GO:0006396 (RNA processing), GO:0008380 (RNA splicing), GO:0006397 (mRNA processing), GO:0016071 (mRNA metabolic process), GO:0000398 (mRNA splicing, via spliceosome), GO:0000375 (RNA splicing, via transesterification reactions), GO:0000377 (RNA splicing, via transesterification reactions with bulged adenosine as nucleophile)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)
Protein: Type II secretion system protein L (T2SS protein L) (E0SLF6)
Organism: Dickeya dadantii (strain 3937) (Erwinia chrysanthemi (strain 3937))
Function: Not known
Subcellular location: Cell inner membrane {ECO:0000256|ARBA:ARBA00004377}; Single-pass membrane protein {ECO:0000256|ARBA:ARBA00004377}.
InterPro domains:
- IPR024230: GspL, cytoplasmic actin-ATPase-like domain (domain) [13-238]
- IPR043129: ATPase, nucleotide binding domain (homologous_superfamily) [12-236]
- IPR025691: GspL periplasmic domain (domain) [246-399]
- IPR007812: Type II secretion system protein GspL (family) [10-397]
Sequence:
MNRAENASGKQQLILRLSTETSDSLEWLIWSVSRHQTLTQGSGTLERLQTVLADYPVMSVRVLVPSTDVTFHSLSLPRQSRRQLLQAIPFMLEEQVASDIDQLHFAVMEMQGDNATVAVVQKSRLRSWLNQCETLGVPVETMVPDVMALPRTDSAWSAISHQNLWLFRLDSGIGMAAEDSWYQSLLAGFQPLPAVHCYSPVPASALTWQPQPVTDLLTLAAQASLPISMDLRQGEYAPVKPWKQAVLPWRNVLIALFAWLLLVLGESVWTHYQLYRQADYWRQESVRMYRKLFPDEKQVVNPRAQMQRHLQEARSGMSGFALTEQMNRLQQLVAQNDGVSLQSLSYDRSRDELRLSLRATSYAQMEQFRQQAQAYFQIPPGEMKQEKDHVEGQLILRSQP
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0140657 (ATP-dependent activity), GO:0016887 (ATP hydrolysis activity), GO:0016787 (hydrolase activity), GO:0016817 (hydrolase activity, acting on acid anhydrides), GO:0016818 (hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides), GO:0016462 (pyrophosphatase activity), GO:0017111 (ribonucleoside triphosphate phosphatase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0043170 (macromolecule metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0019538 (protein metabolic process), GO:0006508 (proteolysis), GO:0051604 (protein maturation), GO:0010467 (gene expression), GO:0016485 (protein processing)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0071944 (cell periphery), GO:0016020 (membrane), GO:0005886 (plasma membrane)
Protein: Kruppel-like transcription factor (Q9WTQ3)
Organism: Rattus norvegicus (Rat)
Function: Not known
Subcellular location: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
InterPro domains:
- IPR013087: Zinc finger C2H2-type (domain) [320-407]
- IPR036236: Zinc finger C2H2 superfamily (homologous_superfamily) [334-403]
Sequence:
MVDHLLPVDETFSSPKCPVGYLGDRLASRQPYHMLPSPISEDDSDVSSPCSCASPDSQAFCSCYSAGPGPEAQGSILDFLLSRATLGSGGASGGVGDGSGPVTWGSWRRASVPVKEEHLCFPEFLSGDPDDVSRPFQPTLEEIEEFLEENMEAEVKEAPESSSRDLEACSQLSAGSHRSHLHPESGGRECRIPPPGGTSGGGTQGAGEGSAHDGPMPVLLQIQPVAVKQEAGAGPASPGQAPESVKVAQLLVNIQGQTFALLPQVVPSSNLNLSSKFVRIAPVPIAAKPIGSGSLGPGPAGLLVGQKFPKNPAAELLKMHKCTFPGCSKMYTKSSHLKAHLRRHTGEKPFACTWPGCGWRFSRSDELSRHRRSHSGVKPYQCPVCEKKFARSDHLSKHIKVHRFPRSSRAVRAIN
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0097159 (organic cyclic compound binding), GO:1901363 (heterocyclic compound binding), GO:0005515 (protein binding), GO:0003676 (nucleic acid binding), GO:0003677 (DNA binding), GO:0001067 (transcription regulatory region nucleic acid binding), GO:0003690 (double-stranded DNA binding), GO:0043565 (sequence-specific DNA binding), GO:0000976 (transcription cis-regulatory region binding), GO:1990837 (sequence-specific double-stranded DNA binding)
Biological Process (BP): GO:0008150 (biological process), GO:0009987 (cellular process), GO:0050896 (response to stimulus), GO:0051716 (cellular response to stimulus), GO:0042221 (response to chemical), GO:0009719 (response to endogenous stimulus), GO:0071495 (cellular response to endogenous stimulus), GO:1901700 (response to oxygen-containing compound), GO:1901698 (response to nitrogen compound), GO:0010033 (response to organic substance), GO:0070887 (cellular response to chemical stimulus), GO:0071417 (cellular response to organonitrogen compound), GO:1901652 (response to peptide), GO:1901699 (cellular response to nitrogen compound), GO:0010243 (response to organonitrogen compound), GO:1901701 (cellular response to oxygen-containing compound), GO:0071310 (cellular response to organic substance), GO:1901653 (cellular response to peptide)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)
Protein: Cysteine protease ATG4a (EC 3.4.22.-) (Autophagy-related protein 4 homolog a) (AtAPG4a) (Protein autophagy 4a) (Q8S929)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins. The protease activity is required for proteolytic activation of ATG8 family proteins: cleaves the C-terminal amino acid of ATG8 proteins to reveal a C-terminal glycine (By similarity). Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. In addition to the protease activity, also mediates delipidation of PE-conjugated ATG8 proteins (By similarity).
Subcellular location: Cytoplasm {ECO:0000250|UniProtKB:Q8BGE6}.
InterPro domains:
- IPR005078: Peptidase C54 (family) [74-461]
- IPR038765: Papain-like cysteine peptidase superfamily (homologous_superfamily) [96-439]
- IPR046792: Peptidase C54, catalytic domain (domain) [138-419]
Sequence:
MKALCDRFVPQQCSSSSKSDTHDKSPLVSDSGPSDNKSKFTLWSNVFTSSSSVSQPYRESSTSGHKQVCTTRNGWTAFVKRVSMASGAIRRFQERVLGPNRTGLPSTTSDVWLLGVCYKISADENSGETDTGTVLAALQLDFSSKILMTYRKGFEPFRDTTYTSDVNWGCMIRSSQMLFAQALLFHRLGRAWTKKSELPEQEYLETLEPFGDSEPSAFSIHNLIIAGASYGLAAGSWVGPYAICRAWESLACKKRKQTDSKNQTLPMAVHIVSGSEDGERGGAPILCIEDATKSCLEFSKGQSEWTPIILLVPLVLGLDSVNPRYIPSLVATFTFPQSVGILGGKPGASTYIVGVQEDKGFYLDPHEVQQVVTVNKETPDVDTSSYHCNVLRYVPLESLDPSLALGFYCRDKDDFDDFCLRALKLAEESNGAPLFTVTQTHTAINQSNYGFADDDSEDEREDDWQML
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0008233 (peptidase activity), GO:0004175 (endopeptidase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044238 (primary metabolic process), GO:0044237 (cellular metabolic process), GO:0061919 (process utilizing autophagic mechanism), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0006807 (nitrogen compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0044248 (cellular catabolic process), GO:0019538 (protein metabolic process), GO:0006914 (autophagy), GO:0036211 (protein modification process), GO:0043412 (macromolecule modification), GO:0006508 (proteolysis)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0005739 (mitochondrion), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)