Protein: Patatin-like phospholipase 1 (EC 3.1.1.4) (PfPATPL1) (W7K139)
Organism: Plasmodium falciparum (isolate NF54)
Function: Hydrolyzes the ester bond of the fatty acyl group attached at the sn-2 position of phospholipids such as phosphatidylcholine. Involved in gametogenesis; however, it is not clear whether it is involved in gametocytes development in host erythrocytes or in gametocyte activation in the mosquito midgut. Involved in gametocyte development in host erythrocytes; however, not involved in gametocytes activation including male gamete exflagellation. Involved in the rounding up of gametocytes following activation in the mosquito midgut; however, not required for gametocyte development in host erythrocytes. Required for exflagellation of activated male gametocytes. Involved in gametocytes egress from host erythrocytes by promoting the relocalization of perforin-like protein PLP2-containing vesicles to the periphery of gametocytes; PLP2 secretion is required for permeabilization of the erythrocyte membrane and thus, promotes gametocyte egress. Dispensable for asexual blood stage development.
Subcellular location: Cytoplasm {ECO:0000269|PubMed:31413195, ECO:0000269|PubMed:31734953}.
InterPro domains:
- IPR016035: Acyl transferase/acyl hydrolase/lysophospholipase (homologous_superfamily) [332-673]
- IPR002641: Patatin-like phospholipase domain (domain) [338-544]
Sequence:
MSDDDDKIYIYSDLFSKNFSDDEKDDSYEREKQVYSGSETQNAENEYSKLRAQNSTILNNYFDNDNIKNVENLKSNDPDQIDLILFPVNKNYYMNLFDGQLIENIHSIKLRKAGFYAIYVENNNNSKWDGIYFGLSRMQVELDYKLITKKNKDGGEYEKRNTSSYDNTESVQNTVGSEKEETENKNEETSNYNSNLNNEINKICKYNLDQTDILLDDSNSERRRNSKFKIKNTNYYDNLMLQNKYTNSILYDDDDDKNNTETYTCTFKTEDQIRVPSQKKKYIYLYNKYDNATLDLNVHTYMSLGMSILCKYSLLYCGKYNHIPRDPYTPFKKPVSILSLDGGGILTISTLLVLNRLEAELRKEIGSDDIKLIDCFDMVCGTSAGGLISLALLREIDLQDVSNMWPSTIKKVFEGNRNIISGIFFEGYDVNNVKDVFLERMGNKFMSSYKKFYCFVTATDVKHKPYKLFLIRNYTHKYNSINAESYDGINKVPLWLAAWATASAPTYLKGPSAEDIKKLGINIKPEIHLVDGALKASNPALIALEECARLNNKNLSTFIKEDLDTLVSIGTGQVPTKLTQSGASSKSASTFEILINSTHLLTRANDTHREVLQRLADRENTYFRFNVPHIGDIEIDSQDVRDFDLISKATQDYLFDEKFYEIKRLAHKLANNYIRSKYL
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0016788 (hydrolase activity, acting on ester bonds), GO:0016298 (lipase activity), GO:0052689 (carboxylic ester hydrolase activity), GO:0004623 (phospholipase A2 activity), GO:0004620 (phospholipase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0044237 (cellular metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0044255 (cellular lipid metabolic process), GO:0006629 (lipid metabolic process), GO:0019637 (organophosphate metabolic process), GO:0006793 (phosphorus metabolic process), GO:0006796 (phosphate-containing compound metabolic process), GO:0046486 (glycerolipid metabolic process), GO:0006644 (phospholipid metabolic process), GO:0006650 (glycerophospholipid metabolic process), GO:0046337 (phosphatidylethanolamine metabolic process), GO:0046471 (phosphatidylglycerol metabolic process), GO:0032048 (cardiolipin metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0005739 (mitochondrion), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)