Protein: Oryzalexin D synthase (EC 1.14.14.112) (EC 1.14.14.123) (Cytochrome P450 76M8) (Ent-cassadiene C11-alpha-hydroxylase 2) (Q6YTF1)
Organism: Oryza sativa subsp. japonica (Rice)
Function: Enzyme of the diterpenoid metabolism involved in the biosynthesis of both phytocassane and the oryzalexin class of phytoalexins. Can hydroxylate syn-pimaradiene, ent-pimaradiene, ent-sandaracopimaradiene, ent-isokaurene, ent-kaurene, and ent-cassadiene, but no activity with syn-stemodene, syn-stemarene, syn-labdatriene, C11-alpha-hydroxy-ent-cassadiene or syn-pimadien-19-oic acid as substrates. Hydroxylates 3-alpha-hydroxy-ent-sandaracopimaradiene at C-7-beta, resulting in a 3-alpha,7-beta-diol corresponding to oryzalexins D.
Subcellular location: Membrane {ECO:0000305}; Single-pass membrane protein {ECO:0000305}.
InterPro domains:
- IPR001128: Cytochrome P450 (family) [35-471]
- IPR002401: Cytochrome P450, E-class, group I (family) [63-465]
- IPR036396: Cytochrome P450 superfamily (homologous_superfamily) [20-492]
- IPR017972: Cytochrome P450, conserved site (conserved_site) [435-444]
Sequence:
MENSQMWLLWGALSVALFFYFSTLRRRYAGGKPLPPGPTPLPLIGNLHLVGGGTFHHKLRDLARVHGPVMTLKLGLATNVVISSREAAIEAYTKYDRHLAARATPDTFRACGFADRSMVFIPSSDPQWKALRGIHASHVFTPRVLAAVRPIRERKVGDLIAYLRAHAGEEVLVGHAMYTGILNMVSFSYFSVDIVDMGSQMARELREVVDDIILVVGKPNVSDFYPFLRPLDLQGLRRWTTKRFNRVFSIMGDIIDRRLAHIRDNKPRHDDFLDSILELMAAGKIDRVNVLNMLFEAFVAGADTMALTLEWVMAELLKNPSVMAKARAELRDVLGDKEIVEEADAARLPYLQAVLKEAMRLHPVGALLLPHFAMEDGVEVGGYAVPKGSTVLFNAWAIMRDAAAWERPDEFVPERFVERTPQLDFRGKDVEFMPFGSGRRLCPGLPLAERVVPFILASMLHTFEWELPGGMTAEELDVSEKFKTANVLAVPLKAVPVLIK
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016491 (oxidoreductase activity), GO:0004497 (monooxygenase activity), GO:0016705 (oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen), GO:0016709 (oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0019748 (secondary metabolic process), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0044550 (secondary metabolite biosynthetic process), GO:1901576 (organic substance biosynthetic process), GO:0006629 (lipid metabolic process), GO:0044255 (cellular lipid metabolic process), GO:0044249 (cellular biosynthetic process), GO:0009404 (toxin metabolic process), GO:0009403 (toxin biosynthetic process), GO:0006720 (isoprenoid metabolic process), GO:0052314 (phytoalexin metabolic process), GO:0008299 (isoprenoid biosynthetic process), GO:0008610 (lipid biosynthetic process), GO:0006721 (terpenoid metabolic process), GO:0052315 (phytoalexin biosynthetic process), GO:0016114 (terpenoid biosynthetic process), GO:0016101 (diterpenoid metabolic process), GO:0016102 (diterpenoid biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0043229 (intracellular organelle), GO:0005783 (endoplasmic reticulum), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)