Protein: Serine-repeat antigen protein 5 (EC 3.4.22.-) (111 kDa antigen) (Serine protease SERA5) (p126) [Cleaved into: p47 (SER36); p56; p50; p18; p25n; p25c] (Q9TY95)
Organism: Plasmodium falciparum (isolate 3D7)
Function: Plays an essential role during the asexual blood stage development by controlling the kinetics of merozoite egress from host erythrocytes. Specifically, prevents premature rupture of the parasitophorous vacuole and host erythrocyte membranes.
Subcellular location: [Serine-repeat antigen protein 5]: Parasitophorous vacuole {ECO:0000269|PubMed:25599609, ECO:0000269|PubMed:28683142, ECO:0000269|PubMed:29716996, ECO:0000305|PubMed:18083098}. Note=Secreted in large amount into the parasitophorous vacuole. {ECO:0000305|PubMed:18083098}.; SUBCELLULAR LOCATION: [p18]: Secreted {ECO:0000250|UniProtKB:P69193}. Note=Secreted during merozoite egress from erythrocytes. {ECO:0000250|UniProtKB:P69193}.; SUBCELLULAR LOCATION: [p25n]: Secreted. Note=Secreted during merozoite egress from erythrocytes. {ECO:0000250|UniProtKB:P69193}.; SUBCELLULAR LOCATION: [p25c]: Secreted {ECO:0000250|UniProtKB:P69193}. Note=Secreted during merozoite egress from erythrocytes. {ECO:0000250|UniProtKB:P69193}.; SUBCELLULAR LOCATION: [p47]: Secreted {ECO:0000250|UniProtKB:P69193}. Cell membrane {ECO:0000269|PubMed:29567995}; Peripheral membrane protein {ECO:0000269|PubMed:29567995}; Extracellular side {ECO:0000250|UniProtKB:P69193}. Note=Secreted during merozoite egress from erythrocytes (By similarity). Colocalizes at the merozoite surface with human VTN (PubMed:29567995). {ECO:0000250|UniProtKB:P69193, ECO:0000269|PubMed:29567995}.; SUBCELLULAR LOCATION: [p50]: Secreted {ECO:0000269|PubMed:18083098, ECO:0000269|PubMed:25599609}. Note=Secreted during egress from host erythrocytes. {ECO:0000269|PubMed:25599609}.; SUBCELLULAR LOCATION: [p56]: Secreted {ECO:0000269|PubMed:18083098}. Note=Secreted during egress from host erythrocytes. {ECO:0000269|PubMed:25599609}.
InterPro domains:
- IPR013128: Peptidase C1A (family) [532-802]
- IPR025661: Cysteine peptidase, asparagine active site (active_site) [782-801]
- IPR000668: Peptidase C1A, papain C-terminal (domain) [569-820]
- IPR038765: Papain-like cysteine peptidase superfamily (homologous_superfamily) [561-822]
- IPR025660: Cysteine peptidase, histidine active site (active_site) [760-770]
Sequence:
MKSYISLFFILCVIFNKNVIKCTGESQTGNTGGGQAGNTGGDQAGSTGGSPQGSTGASPQGSTGASPQGSTGASQPGSSEPSNPVSSGHSVSTVSVSQTSTSSEKQDTIQVKSALLKDYMGLKVTGPCNENFIMFLVPHIYIDVDTEDTNIELRTTLKKTNNAISFESNSGSLEKKKYVKLPSNGTTGEQGSSTGTVRGDTEPISDSSSSSSSSSSSSSSSSSSSSSSSSSSSESLPANGPDSPTVKPPRNLQNICETGKNFKLVVYIKENTLILKWKVYGETKDTTENNKVDVRKYLINEKETPFTNILIHAYKEHNGTNLIESKNYAIGSDIPEKCDTLASNCFLSGNFNIEKCFQCALLVEKENKNDVCYKYLSEDIVSKFKEIKAETEDDDEDDYTEYKLTESIDNILVKMFKTNENNDKSELIKLEEVDDSLKLELMNYCSLLKDVDTTGTLDNYGMGNEMDIFNNLKRLLIYHSEENINTLKNKFRNAAVCLKNVDDWIVNKRGLVLPELNYDLEYFNEHLYNDKNSPEDKDNKGKGVVHVDTTLEKEDTLSYDNSDNMFCNKEYCNRLKDENNCISNLQVEDQGNCDTSWIFASKYHLETIRCMKGYEPTKISALYVANCYKGEHKDRCDEGSSPMEFLQIIEDYGFLPAESNYPYNYVKVGEQCPKVEDHWMNLWDNGKILHNKNEPNSLDGKGYTAYESERFHDNMDAFVKIIKTEVMNKGSVIAYIKAENVMGYEFSGKKVQNLCGDDTADHAVNIVGYGNYVNSEGEKKSYWIVRNSWGPYWGDEGYFKVDMYGPTHCHFNFIHSVVIFNVDLPMNNKTTKKESKIYDYYLKASPEFYHNLYFKNFNVGKKNLFSEKEDNENNKKLGNNYIIFGQDTAGSGQSGKESNTALESAGTSNEVSERVHVYHILKHIKDGKIRMGMRKYIDTQDVNKKHSCTRSYAFNPENYEKCVNLCNVNWKTCEEKTSPGLCLSKLDTNNECYFCYV
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0050789 (regulation of biological process), GO:0065007 (biological regulation), GO:0048583 (regulation of response to stimulus), GO:0002682 (regulation of immune system process), GO:0050776 (regulation of immune response)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0018995 (host cellular component), GO:0043226 (organelle), GO:0005576 (extracellular region), GO:0033643 (host cell part), GO:0043657 (host cell), GO:0043227 (membrane-bounded organelle), GO:0043230 (extracellular organelle), GO:0065010 (extracellular membrane-bounded organelle), GO:0033646 (host intracellular part), GO:0043656 (host intracellular region), GO:0020003 (symbiont-containing vacuole), GO:0033655 (host cell cytoplasm part), GO:0030430 (host cell cytoplasm)