Protein: Pheophorbide a oxygenase, chloroplastic (AtPaO) (Pheide a oxygenase) (EC 1.14.15.17) (Accelerated cell death 1) (Lethal leaf-spot 1 homolog) (Lls1) (Q9FYC2)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Catalyzes the key reaction of chlorophyll catabolism, porphyrin macrocycle cleavage of pheophorbide a (pheide a) to a primary fluorescent catabolite (pFCC). Works in a two-step reaction with red chlorophyll catabolite reductase (RCCR). Creates the intermediate RCC through the opening of the porphyrin macrocycle by the introduction of one atom of molecular oxygen at the alpha-methine bridge. Seems to be specific for pheide a. Belongs to the chlorophyll catabolic enzymes (CCEs).
Subcellular location: Plastid, chloroplast thylakoid membrane {ECO:0000269|PubMed:22366162}.
InterPro domains:
- IPR017941: Rieske [2Fe-2S] iron-sulphur domain (domain) [87-200]
- IPR050584: Cholesterol 7-desaturase (family) [24-482]
- IPR036922: Rieske [2Fe-2S] iron-sulphur domain superfamily (homologous_superfamily) [77-225]
- IPR013626: Pheophorbide a oxygenase (domain) [296-390]
Sequence:
MSVVLLSSTSATITKSQSKKIPFLSPTTKFPLKVSISPSRSKLFHNPLRVAAPPSVPTSDSTEEKRIEEEYGGDKEEEGSEFKWRDHWYPVSLVEDLDPNVPTPFQLLGRDLVLWFDRNDQKWAAFDDLCPHRLAPLSEGRLDENGHLQCSYHGWSFGGCGSCTRIPQAATSGPEARAVKSPRACAIKFPTMVSQGLLFVWPDENGWDRANSIEPPRLPDDFDKPEFSTVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKVESSGPWGFQGANDDSPRITAKFVAPCYSMNKIELDAKLPIVGNQKWVIWICSFNIPMAPGKTRSIVCSARNFFQFSVPGPAWWQVVPRWYEHWTSNLVYDGDMIVLQGQEKVFLAKSMESPDYDVNKQYTKLTFTPTQADRFVLAFRNWLRRHGKSQPEWFGSTPSNQPLPSTVLTKRQMLDRFDQHTQVCSSCKGAYNSFQILKKFLVGATVFWAATAGVPSDVQIRLVLAGLSLISAASAYALHEQEKNFVFRDYVHSEIE
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016491 (oxidoreductase activity), GO:0004497 (monooxygenase activity), GO:0016705 (oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen), GO:0016709 (oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0044282 (small molecule catabolic process), GO:0044283 (small molecule biosynthetic process), GO:0046483 (heterocycle metabolic process), GO:0006082 (organic acid metabolic process), GO:0044248 (cellular catabolic process), GO:0044249 (cellular biosynthetic process), GO:0006520 (amino acid metabolic process), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:1901575 (organic substance catabolic process), GO:1901566 (organonitrogen compound biosynthetic process), GO:0016054 (organic acid catabolic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:1901565 (organonitrogen compound catabolic process), GO:0043436 (oxoacid metabolic process), GO:0016053 (organic acid biosynthetic process), GO:0019438 (aromatic compound biosynthetic process), GO:0018130 (heterocycle biosynthetic process), GO:0009063 (amino acid catabolic process), GO:1901361 (organic cyclic compound catabolic process), GO:0042430 (indole-containing compound metabolic process), GO:0009308 (amine metabolic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:1901605 (alpha-amino acid metabolic process), GO:0042435 (indole-containing compound biosynthetic process), GO:0019752 (carboxylic acid metabolic process), GO:0006576 (biogenic amine metabolic process), GO:0006586 (indolalkylamine metabolic process), GO:0006568 (tryptophan metabolic process), GO:0009309 (amine biosynthetic process), GO:1901606 (alpha-amino acid catabolic process), GO:0009074 (aromatic amino acid family catabolic process), GO:0046395 (carboxylic acid catabolic process), GO:0046394 (carboxylic acid biosynthetic process), GO:0046219 (indolalkylamine biosynthetic process), GO:0000162 (tryptophan biosynthetic process), GO:0042401 (biogenic amine biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0009536 (plastid), GO:0009507 (chloroplast), GO:0043231 (intracellular membrane-bounded organelle)