Protein: Omega-3 fatty acid desaturase fat-1 (FAT-1) (EC 1.14.19.-) (FAT-1 N3 desaturase) (Fatty acid desaturase 1) (Fatty acid metabolism 1) (Q9NEQ0)
Organism: Caenorhabditis elegans
Function: Omega-3 fatty acid desaturase that recognizes a range of 18- and 20-carbon omega-6 substrates. Introduces a double bond in the fatty acid chain three carbons away from terminal methyl group to biosynthesize n-3 (omega-3) polyunsaturated fatty acids (PUFAs) endogenously (PUFAs are essential for membrane structure and many cellular and physiological processes). Acts on a number of substrates like linoleoyl-CoA ((9Z,12Z)-octadecadienoyl-CoA, 18:2n-6), dihomo-gamma-linolenoyl-CoA ((8Z,11Z,14Z)-eicosatrienoyl-CoA, 20:3n-6), and arachidonoyl-CoA ((5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA, 20:4n-6), to generate alpha-linolenoyl-CoA ((9Z,12Z,15Z)-octadecatrienoyl-CoA, 18:3n-3), (8Z,11Z,14Z,17Z)-eicosatetraenoyl-CoA (20:4n-3) and (5Z,8Z,11Z,14Z,17Z)-eicosapentaenoyl-CoA (20:5n-3) respectively. Unlike plants, Caenorhabditis elegans desaturases seem to use fatty acyl-CoAs as substrates (By similarity).
Subcellular location: Membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.
InterPro domains:
- IPR012171: Fatty acid desaturase (family) [43-389]
- IPR005804: Fatty acid desaturase domain (domain) [103-351]
Sequence:
MVAHSSEGLSATAPVTGGDVLVDARASLEEKEAPRDVNANTKQATTEEPRIQLPTVDAFRRAIPAHCFERDLVKSIRYLVQDFAALTILYFALPAFEYFGLFGYLVWNIFMGVFGFALFVVGHDCLHGSFSDNQNLNDFIGHIAFSPLFSPYFPWQKSHKLHHAFTNHIDKDHGHVWIQDKDWEAMPSWKRWFNPIPFSGWLKWFPVYTLFGFCDGSHFWPYSSLFVRNSERVQCVISGICCCVCAYIALTIAGSYSNWFWYYWVPLSFFGLMLVIVTYLQHVDDVAEVYEADEWSFVRGQTQTIDRYYGLGLDTTMHHITDGHVAHHFFNKIPHYHLIEATEGVKKVLEPLSDTQYGYKSQVNYDFFARFLWFNYKLDYLVHKTAGIMQFRTTLEEKAKAK
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016491 (oxidoreductase activity), GO:0016705 (oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0050896 (response to stimulus), GO:0044419 (biological process involved in interspecies interaction between organisms), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0000003 (reproduction), GO:0032501 (multicellular organismal process), GO:0009605 (response to external stimulus), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0009607 (response to biotic stimulus), GO:0006950 (response to stress), GO:0071704 (organic substance metabolic process), GO:0051707 (response to other organism), GO:0044238 (primary metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0043207 (response to external biotic stimulus), GO:0006629 (lipid metabolic process), GO:0044283 (small molecule biosynthetic process), GO:0006082 (organic acid metabolic process), GO:0006952 (defense response), GO:0044255 (cellular lipid metabolic process), GO:0044249 (cellular biosynthetic process), GO:0042335 (cuticle development), GO:0098542 (defense response to other organism), GO:0006631 (fatty acid metabolic process), GO:0043436 (oxoacid metabolic process), GO:0016053 (organic acid biosynthetic process), GO:0040002 (collagen and cuticulin-based cuticle development), GO:0008610 (lipid biosynthetic process), GO:0006633 (fatty acid biosynthetic process), GO:0019752 (carboxylic acid metabolic process), GO:0033559 (unsaturated fatty acid metabolic process), GO:0046394 (carboxylic acid biosynthetic process), GO:0072330 (monocarboxylic acid biosynthetic process), GO:0032787 (monocarboxylic acid metabolic process), GO:0030497 (fatty acid elongation), GO:0006636 (unsaturated fatty acid biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)