Protein: Centrosomal protein of 131 kDa (5-azacytidine-induced protein 1) (Pre-acrosome localization protein 1) (Q9UPN4)
Organism: Homo sapiens (Human)
Function: Component of centriolar satellites contributing to the building of a complex and dynamic network required to regulate cilia/flagellum formation. In proliferating cells, MIB1-mediated ubiquitination induces its sequestration within centriolar satellites, precluding untimely cilia formation initiation. In contrast, during normal and ultraviolet or heat shock cellular stress-induced ciliogenesis, its non-ubiquitinated form is rapidly displaced from centriolar satellites and recruited to centrosome/basal bodies in a microtubule- and p38 MAPK-dependent manner. Acts also as a negative regulator of BBSome ciliary trafficking. Plays a role in sperm flagellar formation; may be involved in the regulation of intraflagellar transport (IFT) and/or intramanchette (IMT) trafficking, which are important for axoneme extension and/or cargo delivery to the nascent sperm tail (By similarity). Required for optimal cell proliferation and cell cycle progression; may play a role in the regulation of genome stability in non-ciliogenic cells. Involved in centriole duplication (By similarity). Required for CEP152, WDR62 and CEP63 centrosomal localization and promotes the centrosomal localization of CDK2. Essential for maintaining proper centriolar satellite integrity.
Subcellular location: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000269|PubMed:14654843, ECO:0000269|PubMed:27224062, ECO:0000269|PubMed:30538148, ECO:0000269|PubMed:30804208}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriolar satellite {ECO:0000269|PubMed:26297806, ECO:0000269|PubMed:26616734, ECO:0000269|PubMed:30804208, ECO:0000269|PubMed:31789463}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole {ECO:0000269|PubMed:30804208}. Cytoplasm, cytoskeleton, cilium basal body. Cytoplasmic vesicle, secretory vesicle, acrosome {ECO:0000250|UniProtKB:Q62036}. Note=Colocalized with pericentriolar material protein PCM1 at centriolar satellites. During spermiogenesis, becomes enriched with nephrocystin NPHP1 at the transition zone, a structure at the base of the ciliary axoneme important for regulating traffic into the ciliary compartment. Traffics towards and away from the centrosome/basal body and the transition zone of the ciliary axoneme in a microtubule-dependent manner. Localized at the Golgi-derived acrosome and the centrosome-containing head-tail coupling apparatus (HTCA) (By similarity). Ubiquitinated form is sequestered and colocalized with BBS4, CEP290, PCM1 and PCNT at centriolar satellites in proliferating cells. Colocalized with the pericentriolar material protein PCM1 at centrosome. Traffics towards and away from centriolar satellites and centrosome in a microtubule- and dynein-dependent manner in interphase cells. Displaced from centriolar satellites but still remains associated with the centrosome in response to cellular stress, such as ultraviolet light (UV) radiation or heat shock, in a process that requires p38 MAPK signaling (PubMed:26616734). {ECO:0000250, ECO:0000269|PubMed:26616734}.
InterPro domains:
- IPR030465: Centrosomal protein of 131kDa (family) [7-1077]
Sequence:
MKGTRAIGSVPERSPAGVDLSLTGLPPPVSRRPGSAATTKPIVRSVSVVTGSEQKRKVLEATGPGGSQAINNLRRSNSTTQVSQPRSGSPRPTEPTDFLMLFEGSPSGKKRPASLSTAPSEKGATWNVLDDQPRGFTLPSNARSSSALDSPAGPRRKECTVALAPNFTANNRSNKGAVGNCVTTMVHNRYTPSERAPPLKSSNQTAPSLNNIIKAATCEGSESSGFGKLPKNVSSATHSARNNTGGSTGLPRRKEVTEEEAERFIHQVNQATVTIQRWYRHQVQRRGAGAARLEHLLQAKREEQRQRSGEGTLLDLHQQKEAARRKAREEKARQARRAAIQELQQKRALRAQKASTAERGPPENPRETRVPGMRQPAQELSPTPGGTAHQALKANNTGGGLPAAGPGDRCLPTSDSSPEPQQPPEDRTQDVLAQDAAGDNLEMMAPSRGSAKSRGPLEELLHTLQLLEKEPDVLPRPRTHHRGRYAWASEVTTEDDASSLTADNLEKFGKLSAFPEPPEDGTLLSEAKLQSIMSFLDEMEKSGQDQLDSQQEGWVPEAGPGPLELGSEVSTSVMRLKLEVEEKKQAMLLLQRALAQQRDLTARRVKETEKALSRQLQRQREHYEATIQRHLAFIDQLIEDKKVLSEKCEAVVAELKQEDQRCTERVAQAQAQHELEIKKLKELMSATEKARREKWISEKTKKIKEVTVRGLEPEIQKLIARHKQEVRRLKSLHEAELLQSDERASQRCLRQAEELREQLEREKEALGQQERERARQRFQQHLEQEQWALQQQRQRLYSEVAEERERLGQQAARQRAELEELRQQLEESSSALTRALRAEFEKGREEQERRHQMELNTLKQQLELERQAWEAGRTRKEEAWLLNREQELREEIRKGRDKEIELVIHRLEADMALAKEESEKAAESRIKRLRDKYEAELSELEQSERKLQERCSELKGQLGEAEGENLRLQGLVRQKERALEDAQAVNEQLSSERSNLAQVIRQEFEDRLAASEEETRQAKAELATLQARQQLELEEVHRRVKTALARKEEAVSSLRTQHEAAVKRADHLEELLEQHRRPTPSTK
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0044877 (protein-containing complex binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0051179 (localization), GO:0022414 (reproductive process), GO:0000003 (reproduction), GO:0032501 (multicellular organismal process), GO:0032502 (developmental process), GO:0009987 (cellular process), GO:0019953 (sexual reproduction), GO:0048869 (cellular developmental process), GO:0051641 (cellular localization), GO:0048856 (anatomical structure development), GO:0048870 (cell motility), GO:0031503 (protein-containing complex localization), GO:0051234 (establishment of localization), GO:0071840 (cellular component organization or biogenesis), GO:0032504 (multicellular organism reproduction), GO:0003006 (developmental process involved in reproduction), GO:0033036 (macromolecule localization), GO:0097722 (sperm motility), GO:0022412 (cellular process involved in reproduction in multicellular organism), GO:0007017 (microtubule-based process), GO:0048609 (multicellular organismal reproductive process), GO:0007276 (gamete generation), GO:0007281 (germ cell development), GO:0044085 (cellular component biogenesis), GO:0048468 (cell development), GO:0030154 (cell differentiation), GO:0048515 (spermatid differentiation), GO:0120316 (sperm flagellum assembly), GO:0051649 (establishment of localization in cell), GO:0007288 (sperm axoneme assembly), GO:0000226 (microtubule cytoskeleton organization), GO:0030317 (flagellated sperm motility), GO:0007283 (spermatogenesis), GO:0045184 (establishment of protein localization), GO:0042073 (intraciliary transport), GO:0007018 (microtubule-based movement), GO:0099118 (microtubule-based protein transport), GO:0046907 (intracellular transport), GO:0006810 (transport), GO:0001539 (cilium or flagellum-dependent cell motility), GO:0070727 (cellular macromolecule localization), GO:0016043 (cellular component organization), GO:0010970 (transport along microtubule), GO:0030030 (cell projection organization), GO:0071705 (nitrogen compound transport), GO:0003341 (cilium movement), GO:0030705 (cytoskeleton-dependent intracellular transport), GO:0071702 (organic substance transport), GO:0007286 (spermatid development), GO:0099111 (microtubule-based transport), GO:0001578 (microtubule bundle formation), GO:0022607 (cellular component assembly), GO:0006886 (intracellular protein transport), GO:0015031 (protein transport), GO:0008104 (protein localization), GO:0006996 (organelle organization), GO:0048232 (male gamete generation), GO:0098840 (protein transport along microtubule), GO:0060285 (cilium-dependent cell motility), GO:0070925 (organelle assembly), GO:0030031 (cell projection assembly), GO:0120036 (plasma membrane bounded cell projection organization), GO:0035082 (axoneme assembly), GO:0060294 (cilium movement involved in cell motility), GO:0007010 (cytoskeleton organization), GO:0044782 (cilium organization), GO:0060271 (cilium assembly), GO:0120031 (plasma membrane bounded cell projection assembly), GO:0044458 (motile cilium assembly), GO:1905515 (non-motile cilium assembly)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005815 (microtubule organizing center), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0045171 (intercellular bridge), GO:0034451 (centriolar satellite), GO:0005813 (centrosome), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005856 (cytoskeleton), GO:0015630 (microtubule cytoskeleton)