Protein: Serine/threonine-protein kinase plo1 (EC 2.7.11.21) (Polo kinase plo1) (P50528)
Organism: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Function: Required to form a bipolar spindle, the actin ring and septum. Functions upstream of the whole septum formation pathway, including actin ring formation (regulated by late septation genes) and septal material deposition (regulated by early septation genes). Behaves as a 'septum-promoting factor', and could also be involved in inducing other late events of cell division. Together with moa1, acts as a regulator of kinetochore function during meiosis I: required both for mono-orientation of kinetochores on sister chromosomes and protection of centromeric cohesin from separase-mediated cleavage.
Subcellular location: Chromosome, centromere, kinetochore {ECO:0000269|PubMed:25533956}. Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body {ECO:0000269|PubMed:11676915, ECO:0000269|PubMed:12615979, ECO:0000269|PubMed:12815070, ECO:0000269|PubMed:16823372}.
InterPro domains:
- IPR011009: Protein kinase-like domain superfamily (homologous_superfamily) [41-325]
- IPR036947: POLO box domain superfamily (homologous_superfamily) [482-671]
- IPR033695: Second polo-box domain (domain) [599-676]
- IPR008271: Serine/threonine-protein kinase, active site (active_site) [159-171]
- IPR033701: First polo-box domain (domain) [490-580]
- IPR017441: Protein kinase, ATP binding site (binding_site) [47-73]
- IPR000719: Protein kinase domain (domain) [41-296]
- IPR000959: POLO box domain (domain) [493-678]
Sequence:
MASVAIKEPKTAITPKKKSKASRLCFTPPTNLHNNKKNIFYTRYDCIGEGGFARCFRVKDNYGNIYAAKVIAKRSLQNDKTKLKLFGEIKVHQSMSHPNIVGFIDCFEDSTNIYLILELCEHKSLMELLRKRKQLTEPEVRYLMMQILGALKYMHKKRVIHRDLKLGNIMLDESNNVKIGDFGLAALLMDDEERKMTICGTPNYIAPEILFNSKEGHSFEVDLWSAGVVMYALLIGKPPFQDKEVKTIYRKIKANSYSFPSNVDISAEAKDLISSLLTHDPSIRPSIDDIVDHEFFHTGYMASTLPDEILHSMPIWPSSQSKSSFQRNLDFVASASGVGFGNSAGVEKNKPYALRTDEVDNDRILPSVLSPRDRVNPVMKIGPETKPVPSKLSTALHAARKSTDGSLGSRVKVLREESQSFVPTKSAVTEQVEPIQLIRSLSANTVSRLSKVGNMKSDIWISVKKTALKIGMALEAHTHALTSEDADSEPVLFITKWVDYSNKYGLGYQLSDESVGVHFNDDTSLLFSADEEVVEYALHPKDTEIKPYIYPASKVPESIRSKLQLLKHFKSYMGQNLSKAVQDESFEKPKNSTSNTMLFMQHYLRTRQAIMFRLSNGIFQFNFLDHRKVVISSTARKIIVLDKERERVELPLQEASAFSEDLRSRLKYIRETLESWASKMEVS
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0005515 (protein binding), GO:0004672 (protein kinase activity), GO:0016772 (transferase activity, transferring phosphorus-containing groups), GO:0016773 (phosphotransferase activity, alcohol group as acceptor), GO:0004674 (protein serine/threonine kinase activity), GO:0016301 (kinase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0051179 (localization), GO:0050789 (regulation of biological process), GO:0022414 (reproductive process), GO:0000003 (reproduction), GO:0065007 (biological regulation), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0051641 (cellular localization), GO:0051321 (meiotic cell cycle), GO:0022402 (cell cycle process), GO:0007049 (cell cycle), GO:0071840 (cellular component organization or biogenesis), GO:0033036 (macromolecule localization), GO:0050794 (regulation of cellular process), GO:1903046 (meiotic cell cycle process), GO:0048523 (negative regulation of cellular process), GO:0061982 (meiosis I cell cycle process), GO:0044085 (cellular component biogenesis), GO:1903047 (mitotic cell cycle process), GO:0051129 (negative regulation of cellular component organization), GO:0070192 (chromosome organization involved in meiotic cell cycle), GO:0045132 (meiotic chromosome segregation), GO:0007062 (sister chromatid cohesion), GO:0008608 (attachment of spindle microtubules to kinetochore), GO:0010948 (negative regulation of cell cycle process), GO:0044087 (regulation of cellular component biogenesis), GO:0051726 (regulation of cell cycle), GO:0007059 (chromosome segregation), GO:0061983 (meiosis II cell cycle process), GO:0140013 (meiotic nuclear division), GO:0051316 (attachment of spindle microtubules to kinetochore involved in meiotic chromosome segregation), GO:0070727 (cellular macromolecule localization), GO:0045786 (negative regulation of cell cycle), GO:0016043 (cellular component organization), GO:0051128 (regulation of cellular component organization), GO:0010564 (regulation of cell cycle process), GO:0000278 (mitotic cell cycle), GO:0098813 (nuclear chromosome segregation), GO:0140014 (mitotic nuclear division), GO:0007135 (meiosis II), GO:0007127 (meiosis I), GO:0022607 (cellular component assembly), GO:0045143 (homologous chromosome segregation), GO:0010639 (negative regulation of organelle organization), GO:0051225 (spindle assembly), GO:0051983 (regulation of chromosome segregation), GO:0070601 (centromeric sister chromatid cohesion), GO:1901987 (regulation of cell cycle phase transition), GO:0045930 (negative regulation of mitotic cell cycle), GO:1905819 (negative regulation of chromosome separation), GO:0031134 (sister chromatid biorientation), GO:0033043 (regulation of organelle organization), GO:0051177 (meiotic sister chromatid cohesion), GO:0008104 (protein localization), GO:0006996 (organelle organization), GO:0000070 (mitotic sister chromatid segregation), GO:0045144 (meiotic sister chromatid segregation), GO:1901988 (negative regulation of cell cycle phase transition), GO:0051783 (regulation of nuclear division), GO:0033365 (protein localization to organelle), GO:0051784 (negative regulation of nuclear division), GO:0010965 (regulation of mitotic sister chromatid separation), GO:0033045 (regulation of sister chromatid segregation), GO:1901991 (negative regulation of mitotic cell cycle phase transition), GO:2000816 (negative regulation of mitotic sister chromatid separation), GO:0033046 (negative regulation of sister chromatid segregation), GO:0048285 (organelle fission), GO:0051276 (chromosome organization), GO:1902099 (regulation of metaphase/anaphase transition of cell cycle), GO:0033048 (negative regulation of mitotic sister chromatid segregation), GO:0031577 (spindle checkpoint signaling), GO:0045841 (negative regulation of mitotic metaphase/anaphase transition), GO:0033047 (regulation of mitotic sister chromatid segregation), GO:1901990 (regulation of mitotic cell cycle phase transition), GO:0000819 (sister chromatid segregation), GO:1902100 (negative regulation of metaphase/anaphase transition of cell cycle), GO:2001251 (negative regulation of chromosome organization), GO:0033044 (regulation of chromosome organization), GO:0071174 (mitotic spindle checkpoint signaling), GO:0034502 (protein localization to chromosome), GO:0000280 (nuclear division), GO:0007094 (mitotic spindle assembly checkpoint signaling), GO:0071173 (spindle assembly checkpoint signaling), GO:0030071 (regulation of mitotic metaphase/anaphase transition), GO:0071459 (protein localization to chromosome, centromeric region), GO:1903083 (protein localization to condensed chromosome), GO:0034501 (protein localization to kinetochore)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005815 (microtubule organizing center), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005829 (cytosol), GO:0005737 (cytoplasm), GO:0005816 (spindle pole body), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0044732 (mitotic spindle pole body), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005856 (cytoskeleton), GO:0005634 (nucleus), GO:0015630 (microtubule cytoskeleton)