Protein: Zinc finger protein zfp-1 (Protein AF-10 homolog) (P34447)
Organism: Caenorhabditis elegans
Function: Recruits the histone methyltransferase dot-1.1 to chromatin to methylate 'Lys-79' of histone H3 and activate transcription. Recognizes and binds histone H3 methylated at 'Lys-4' (H3K4me) at the promoters of target genes. During stress, the zfp-1-dot-1.1 complex also plays a role in the deubiquitination of histone H2B sites, which negatively modulates the RNA polymerase II-induced transcription of highly expressed genes. In response to stress, binds to the pdk-1 promoter to negatively regulate pdk-1 expression, which negatively modulates daf-16/FOXO-mediated gene expression. Thus, most likely via this mechanism, in response to stress, it confers a protective role against neuronal necrosis. Plays a role in Insulin/IGF-1-like signaling (IIS)- and diet restriction-mediated lifespan extension by controlling daf-16/FOXO and pha-4/FOXA recruitment to target promoters. May negatively regulate the expression of genes required for vulval development. May play a role in axon guidance in D-type motor neurons. May suppress sensitivity to RNAi.
Subcellular location: Nucleus {ECO:0000269|PubMed:23263989, ECO:0000305|PubMed:21980302, ECO:0000305|PubMed:23806335}. Chromosome {ECO:0000269|PubMed:23263989, ECO:0000305|PubMed:23806335}. Note=Localizes to all six condensed chromosomes (PubMed:23263989). zfp-1 and dot-1.1 colocalize to promoters of highly expressed genes (PubMed:23806335). {ECO:0000269|PubMed:23263989, ECO:0000269|PubMed:23806335}.
InterPro domains:
- IPR050701: Histone Modification Regulator (family) [8-645]
- IPR049773: AF10-like, coiled-coil domain (domain) [593-654]
- IPR011011: Zinc finger, FYVE/PHD-type (homologous_superfamily) [7-59]
- IPR049781: AF-10/AF-17, PHD finger (domain) [7-54]
- IPR034732: Extended PHD (ePHD) domain (domain) [69-186]
- IPR019787: Zinc finger, PHD-finger (domain) [5-185]
- IPR013083: Zinc finger, RING/FYVE/PHD-type (homologous_superfamily) [2-188]
- IPR001965: Zinc finger, PHD-type (domain) [7-186]
Sequence:
MKEMVGGCCVCADENGWTDNPLIYCDGENCEVAVHQGCYGIQEVPEGEWFCAKCTKASAMMPGSINEATFCCQLCPFDYGALKKTDRNGWAHVICALYIPEVRFGNVHSMEPVILNDVPTDKFNKLCYICNEERPNDAKKGACMSCNKSTCKRSFHVTCAQRKGLLCEEGAISRNVKYCGYCENHLKKAINDPAIKVIPACPPVQRLSKEQDKKKTVLLTSLPLPPPAPRLHMLADPLPIKSNKVNNVLGLGSAINAVSLEERPASGSTVNSGIFVPPPTAFSPPLTTSSRSSVAQDPSPPLTINKNSLSSSGPLIPSTAHLSATTASATPIMANGSTLPPSSETTVGTHCLQQLQIQSAAAAAITQNQIGPSELNGYPAASQLSSFMHEIPARNTTSVASLLPPGAAEYHLNGSGDEEKTVKAVLTAPLTKAKRIRDSKNDMMDKTHKRPRANARPPAVLGSMSSGSSGGTVGKSPSMQRLQNLVAPIVSETVTDFQRDRVADRTAAERRAAAAQSQPSTSTNGGPNVTIPAVVEVHTNSTNSTNHQNNGLTQNAPASTSMQAGTSSNDGVISQNGTSSTSQSNRLNLPSFMEQLLERQWDQGSSLLMANAHFDVAQLLSCLFQLKSENFRLEENLSGLRKRRDHLFALNSRLAEVNTLDVSKRQRSDGLLLQQQIAHHLDPTSIVPKAEVHKQEPLSAPTSVPLPANHSSSLFEDIKAPKATYSRKTPSNIPLTVPLSTAATALTTTTAASSGAPVNSNIQNHRATPSTAGAPMAATPIMTAVTSANELAALSPERAQALLNMYRMPLDANVAAQLSMITNFPGQVNPSLFSRLLAVNMMNGGLQPNGQPLSALPPPTSATPNGK
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0032502 (developmental process), GO:0032501 (multicellular organismal process), GO:0048856 (anatomical structure development), GO:0007275 (multicellular organism development), GO:0042303 (molting cycle), GO:0022404 (molting cycle process), GO:0009791 (post-embryonic development), GO:0042395 (ecdysis, collagen and cuticulin-based cuticle), GO:0010171 (body morphogenesis), GO:0002164 (larval development), GO:0018996 (molting cycle, collagen and cuticulin-based cuticle), GO:0002119 (nematode larval development), GO:0040024 (dauer larval development)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005634 (nucleus)