Protein: E3 ubiquitin-protein ligase RNFT1 (EC 2.3.2.27) (Protein PTD016) (RING finger and transmembrane domain-containing protein 1) (Q5M7Z0)
Organism: Homo sapiens (Human)
Function: E3 ubiquitin-protein ligase that acts in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway, which targets misfolded proteins that accumulate in the endoplasmic reticulum (ER) for ubiquitination and subsequent proteasome-mediated degradation. Protects cells from ER stress-induced apoptosis.
Subcellular location: Endoplasmic reticulum membrane {ECO:0000269|PubMed:27485036}; Multi-pass membrane protein {ECO:0000305}.
InterPro domains:
- IPR017907: Zinc finger, RING-type, conserved site (conserved_site) [390-399]
- IPR013083: Zinc finger, RING/FYVE/PHD-type (homologous_superfamily) [360-433]
- IPR044235: E3 ubiquitin-protein ligase 1/2 (family) [44-435]
- IPR001841: Zinc finger, RING-type (domain) [374-413]
Sequence:
MPLFLLSLPTPPSASGHERRQRPEAKTSGSEKKYLRAMQANRSQLHSPPGTGSSEDASTPQCVHTRLTGEGSCPHSGDVHIQINSIPKECAENASSRNIRSGVHSCAHGCVHSRLRGHSHSEARLTDDTAAESGDHGSSSFSEFRYLFKWLQKSLPYILILSVKLVMQHITGISLGIGLLTTFMYANKSIVNQVFLRERSSKIQCAWLLVFLAGSSVLLYYTFHSQSLYYSLIFLNPTLDHLSFWEVFWIVGITDFILKFFFMGLKCLILLVPSFIMPFKSKGYWYMLLEELCQYYRTFVPIPVWFRYLISYGEFGNVTRWSLGILLALLYLILKLLEFFGHLRTFRQVLRIFFTQPSYGVAASKRQCSDVDDICSICQAEFQKPILLICQHIFCEECMTLWFNREKTCPLCRTVISDHINKWKDGATSSHLQIY
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0140096 (catalytic activity, acting on a protein), GO:0005515 (protein binding), GO:0019787 (ubiquitin-like protein transferase activity), GO:0004842 (ubiquitin-protein transferase activity), GO:0061659 (ubiquitin-like protein ligase activity), GO:0061630 (ubiquitin protein ligase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0065007 (biological regulation), GO:0050789 (regulation of biological process), GO:0009987 (cellular process), GO:0048519 (negative regulation of biological process), GO:0023057 (negative regulation of signaling), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0050794 (regulation of cellular process), GO:0048583 (regulation of response to stimulus), GO:0023051 (regulation of signaling), GO:0071704 (organic substance metabolic process), GO:0048523 (negative regulation of cellular process), GO:0009056 (catabolic process), GO:0048585 (negative regulation of response to stimulus), GO:0044238 (primary metabolic process), GO:0009968 (negative regulation of signal transduction), GO:0010648 (negative regulation of cell communication), GO:1901564 (organonitrogen compound metabolic process), GO:0010646 (regulation of cell communication), GO:0044248 (cellular catabolic process), GO:0019538 (protein metabolic process), GO:0009966 (regulation of signal transduction), GO:1901575 (organic substance catabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0036211 (protein modification process), GO:0043112 (receptor metabolic process), GO:0009057 (macromolecule catabolic process), GO:0043412 (macromolecule modification), GO:1901565 (organonitrogen compound catabolic process), GO:0006508 (proteolysis), GO:0044265 (cellular macromolecule catabolic process), GO:0030163 (protein catabolic process), GO:0030111 (regulation of Wnt signaling pathway), GO:0030178 (negative regulation of Wnt signaling pathway), GO:0032801 (receptor catabolic process), GO:0070647 (protein modification by small protein conjugation or removal), GO:0043632 (modification-dependent macromolecule catabolic process), GO:0051603 (proteolysis involved in protein catabolic process), GO:0032446 (protein modification by small protein conjugation), GO:0019941 (modification-dependent protein catabolic process), GO:0016567 (protein ubiquitination), GO:0006511 (ubiquitin-dependent protein catabolic process), GO:0051865 (protein autoubiquitination), GO:0000209 (protein polyubiquitination), GO:0070936 (protein K48-linked ubiquitination)
Cellular Component (CC): GO:0005575 (cellular component), GO:0032991 (protein-containing complex), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0140534 (endoplasmic reticulum protein-containing complex), GO:0016020 (membrane), GO:0098796 (membrane protein complex), GO:0031984 (organelle subcompartment), GO:1902494 (catalytic complex), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0031090 (organelle membrane), GO:1990234 (transferase complex), GO:0043229 (intracellular organelle), GO:0098827 (endoplasmic reticulum subcompartment), GO:0042175 (nuclear outer membrane-endoplasmic reticulum membrane network), GO:0005783 (endoplasmic reticulum), GO:0000151 (ubiquitin ligase complex), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0005789 (endoplasmic reticulum membrane)