Protein: Membrane-anchored lipid-binding protein LAM6 (Lipid transfer at contact site protein 1) (Lipid transfer protein anchored at membrane contact sites 1) (Q08001)
Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Function: Involved in regulation of various organellar membrane contact sites. May be involved in sterol transfer between intracellular membranes. Selectively transports sterols between membranes in vitro. Involved in stress-dependent formation of sterol-enriched vacuolar membrane domains.
Subcellular location: Endoplasmic reticulum membrane {ECO:0000269|PubMed:26001273}; Single-pass membrane protein {ECO:0000255}. Note=Localizes to intracellular puncta representing endoplasmic reticulum-mitochondrion membrane contact sites (ERMES), nucleus-vacuole junctions (NVJ) and vacuole-mitochondria patches (vCLAMP) (PubMed:14562095, PubMed:25987606, PubMed:26001273, PubMed:26119743). Localization to ER-mitochondria contacts is dependent on TOM70/TOM71, and localization to ER-vacuole junctions on VAC8 (PubMed:25987606). {ECO:0000269|PubMed:14562095, ECO:0000269|PubMed:25987606, ECO:0000269|PubMed:26001273, ECO:0000269|PubMed:26119743}.
InterPro domains:
- IPR004182: GRAM domain (domain) [164-269]
- IPR031968: VASt domain (domain) [403-576]
- IPR051482: Cholesterol transport domain-containing protein (family) [64-662]
- IPR011993: PH-like domain superfamily (homologous_superfamily) [161-268]
Sequence:
MWGDSMRELGDAMDNELNAVKPVVEEGGMDGARKFIKGKSFQKSSTEHMLISPGRDGSVPLNGLKSSPADPHLSDVNSILDNHRGGGETALTSVNNIIMATSTNGDSDGVDGDAKRPSISNCSSRSSFFDTVLSTFSLKSNSQDTVTNEVKNIEVQFASEEANKKFRQMFKPLAPNTRLITDYFCYFHREFPYQGRIYLSNTHLCFNSTVLNWMAKLQIPLNEIKYLDKVTTNSSAISVETVTNRYTFSGFIARDEVFQLITRVWSKENLTNINDVLEVDERVSKKKGISSTPSSIFNNVSTNAYNDFISTTTTEPTSRASYMSENDMLIEEAIRSVDDYMGTPRASPSSSSSSSSSSSSLGSSTTYYCRPVYRLKPNAPFQYEGPFHVQETMDFPYKPEANNEYVLLERQFSVPPGLLFIMMFNEDNPVFELSFLKTQDSSNISHIGTFEKVNKDGQHYREFQYTKQLHFPVGPKSTNCEVAEILLHCDWERYINVLSITRTPNVPSGTSFSTRTRYMFRWDDQGQGCILKISFWVDWNASSWIKPMVESNCKNGQISATKDLVKLVEEFVEKYVELSKEKADTLKPLPSVTSFGSPRKVAAPELTMVQPESKPEAEAEISEIGSDRWRFNWVNIIILVLLVLNLLYLMKLNKKMDKLTNLMTHKDEVVAHATLLDIPAKVQWSRPRRGDVL
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005215 (transporter activity), GO:0097159 (organic cyclic compound binding), GO:0008289 (lipid binding), GO:0005319 (lipid transporter activity), GO:0005496 (steroid binding), GO:0120013 (lipid transfer activity), GO:0015248 (sterol transporter activity), GO:0120015 (sterol transfer activity), GO:0032934 (sterol binding)
Biological Process (BP): GO:0008150 (biological process), GO:0009987 (cellular process), GO:0051179 (localization), GO:0051234 (establishment of localization), GO:0071840 (cellular component organization or biogenesis), GO:0033036 (macromolecule localization), GO:0010876 (lipid localization), GO:0016043 (cellular component organization), GO:0006810 (transport), GO:0006869 (lipid transport), GO:0071702 (organic substance transport), GO:0061024 (membrane organization), GO:0120009 (intermembrane lipid transfer), GO:0015850 (organic hydroxy compound transport), GO:0015918 (sterol transport)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0044232 (organelle membrane contact site), GO:0043229 (intracellular organelle), GO:0005783 (endoplasmic reticulum), GO:0043227 (membrane-bounded organelle), GO:0044233 (mitochondria-associated endoplasmic reticulum membrane), GO:0043231 (intracellular membrane-bounded organelle)