Protein: Protein xmas (Q9U3V9)
Organism: Drosophila melanogaster (Fruit fly)
Function: Involved in mRNA export and mRNA coupled transcription activation. Component of the nuclear pore complex (NPC)-associated TREX-2/AMEX complex (anchoring and mRNA export complex) which functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket), thereby enabling the export of mRNAs to the cytoplasm through the nuclear pores. The TREX-2/AMEX complex also functions with the transcriptional coactivator SAGA/TFTC complex, to anchor a subset of transcription sites to the nuclear pore complex basket in order to achieve efficient transcription and export of their resulting mRNAs. Within the complex, required for localization of e(y)2 to the nuclear periphery.
Subcellular location: Nucleus, nucleoplasm {ECO:0000269|PubMed:28472469}. Nucleus {ECO:0000269|PubMed:29779104, ECO:0000269|Ref.7}. Nucleus membrane {ECO:0000269|PubMed:18034162, ECO:0000269|PubMed:27016737, ECO:0000269|PubMed:28472469, ECO:0000269|PubMed:33602059}; Peripheral membrane protein {ECO:0000269|PubMed:18034162, ECO:0000269|PubMed:27016737, ECO:0000269|PubMed:28472469, ECO:0000269|PubMed:33602059}. Cytoplasm {ECO:0000269|PubMed:33602059}. Note=Localizes to nuclear periphery, often in contact with the nuclear pore complex (NPC). {ECO:0000269|PubMed:18034162, ECO:0000269|PubMed:27016737}.
InterPro domains:
- IPR035979: RNA-binding domain superfamily (homologous_superfamily) [7-82]
- IPR012677: Nucleotide-binding alpha-beta plait domain superfamily (homologous_superfamily) [5-90]
- IPR005062: SAC3/GANP/THP3, conserved domain (domain) [205-502]
- IPR045107: SAC3/GANP/THP3 (family) [10-1139]
- IPR000504: RNA recognition motif domain (domain) [12-83]
- IPR000717: Proteasome component (PCI) domain (domain) [342-525]
- IPR034366: Protein xmas, RNA recognition motif (domain) [12-82]
Sequence:
MAEPRPGGYNYKTLLCRNIPELFLDKYVARSHFGRFGTLVNFVLRPRRMTCTVSYASEDEAARALLDGASFQGHLFDISYADNETAPAQKTEEWVDPDIQAELSALQSGWRNEYGSGKPIKKPQNGSSGSGGSSMLPAIPVGPATAPVSRDRTPAQLRDLENMMRRPAHTSEEKFSVLDARDKLLRLNRTQHKLSGATQGHCADMCPEKERVLREFQRQVAYYELQPGSDELICHERALKQYSRSSADQETPLPHELRNETALHMTMSYLMHEIMDISERQDPQSHMGDWFHFVWDRTRSIRKEITQQELCSLGAVKLVEQCARFHIHCAARLVDADPSVFDSKINAENLTKCLQTLKYMYHDLRIKGVPCPKEAEFRGYIVLLNLADANFLWDIGQLPAELQSCPEVRQAIQFYLALQDTNFVRFFQLLADKDTSYLSACILVNYFTRLRVLGLHRLIQAYRSPRKDEVSSLPLSYIAELLSFASEQEAADFVQHYGLQINEAGRVVLSRMHTVETEYKLPRQYELVEVKRVKSVGEVVSGEPLPPRDLYLNHRPHNSFDDYGMLKSIAWTAKDQLAGMQQEEMQPQMPSQPPAVSKHSDTLFKVPMQPDGTAAGFGVFAAAAVPPASSIDGFSFVLPKSRAQEFQEQAPATQQRRAQEEAKHQALQVAIAAAKKREAELMAIHEAKVAEAERVRQQKLRERQEQQRRQQQELEEQRQREQEKLQLEKERQLKLEQLFFVQQQEREAHKQTRTLELYQEIFQDTLAEICQSEFMSHSRACRSYESMLDSITRDLVERQMEQSIYELGVMRVCIRRWRKYRRTQQEKDTLFNQLPLSFGAENPEGVVNKRSMEDSLRLSRRYRLGEPCDYGKLLAGLEEHSWLKLDLWHVLDKCLPVAQPGARRFYKLLISLSGGQEGLQLNCDLDRGLLQQPQSPDARFVDGGYIRGFSQGIGLSVMKIRDDDHDWKATDLAEANGIICLIGLDDIRLLPDRLKPLLQASRCHDVAVIVQHPANTAFVQPDIPLQELCLRSFNIFRLRKSGNNRQRLMIALESAVKFLAKATERKRVGHLHQVETREYLLVNLGSELFRRLKYAAEHDTAIRSDTQLNPQRCVDLFNEAVHRLQLVAGEDLSDWPQFPEELRVFVQPLPIESSLNTNRLEHFEPGWHLPERRQRIVQLLERCKLPKMPVLPRSSSLAEAQCQWVLDYAQISQQEDCVEQIALQAIKILQYDTDDYLNFVEYLAGERMQYILRQERNPPQGIVYNTKTLKRRFLSAWYYEFREPQIYEPVPAEENAQMLEKQPSSQAEVQQLDFDEITSKAEAVLKRFHQRQDERHTLRELNRSHKSRKRRDASDHKHAMSSLVSALKAARSDYQGKSSAVCRLVAQMVEADQVKYQWTPNLTSLIDRKDASTRHQWRPHVNHSTRHARKLPVQSISFLSRCLAKKGEHAGLTSCVLSVKTRYAPKTLHGRELLIWRQSSMRPLLLPSSNLWTGVLSKRHYVPRLRPAEEDEEVERRAVPQLRITKEQSEEEEHQLQRRKDGSRLRNECSEFYLPMGEQDYSEREEENRGHRQAYVPSELMTPELATKWQTTSVTDRDRQLLNLEQQMKTQPSVRMTTQTWFSGNHRRYRSRRSGAKRYHVVSELEGWRRSSNHQPVPVFQGQPGEQEHLRHASHKASARSMPDGQERCQMVWEQKKRSPWHRTEVTNSNKVRMPRIRAAKSAVMMAQEARNKHHRLITKKLVYRPRRTVNAVQAEETEDQDTHHRHHGGGQKMSKRAPERALLKQHLADLLAVSKPEDSFRIGYQPNAPTRQLLEQGKCYVSLRREQFIHLHPVRPNSLILAVNLEVKESPRQPVTTLHRRDGAKRPRLVEDVVKPSLITVIRQSAATWDHNGRKVPAKKSNLVGMPSKEHAYQRRLVRNTSTLEAIVKAQAKPRSEPKSPSSATDYHRVASQKLPHVTSKADISKAADPFKDLDKPRIKEKEVASSWKQAYVVRSKMYDAITPYRRRAYPGKKCITKDKTDGYFLAKRSSAAAAGSSKKVKPPKQPVVSPKVQVPSVLHKKKSRESLGPQTTKTGKL
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:1901360 (organic cyclic compound metabolic process), GO:1901576 (organic substance biosynthetic process), GO:0044085 (cellular component biogenesis), GO:0006139 (nucleobase-containing compound metabolic process), GO:0046483 (heterocycle metabolic process), GO:0044249 (cellular biosynthetic process), GO:0016043 (cellular component organization), GO:0006725 (cellular aromatic compound metabolic process), GO:0034641 (cellular nitrogen compound metabolic process), GO:0043170 (macromolecule metabolic process), GO:0009059 (macromolecule biosynthetic process), GO:1901362 (organic cyclic compound biosynthetic process), GO:0090304 (nucleic acid metabolic process), GO:0019438 (aromatic compound biosynthetic process), GO:0018130 (heterocycle biosynthetic process), GO:0044271 (cellular nitrogen compound biosynthetic process), GO:0043933 (protein-containing complex organization), GO:0010467 (gene expression), GO:0034654 (nucleobase-containing compound biosynthetic process), GO:0022607 (cellular component assembly), GO:0016070 (RNA metabolic process), GO:0065003 (protein-containing complex assembly), GO:0071824 (protein-DNA complex subunit organization), GO:0032774 (RNA biosynthetic process), GO:0097659 (nucleic acid-templated transcription), GO:0065004 (protein-DNA complex assembly), GO:0006351 (DNA-templated transcription), GO:0006354 (DNA-templated transcription elongation), GO:0006368 (transcription elongation by RNA polymerase II), GO:0006366 (transcription by RNA polymerase II)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0031974 (membrane-enclosed lumen), GO:0005654 (nucleoplasm), GO:0043229 (intracellular organelle), GO:0043233 (organelle lumen), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle), GO:0070013 (intracellular organelle lumen), GO:0031981 (nuclear lumen), GO:0005634 (nucleus)