Protein: Shugoshin 2A (Shugoshin-2) (Shugoshin-like 2) (Q7TSY8)
Organism: Mus musculus (Mouse)
Function: Cooperates with PPP2CA to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I. Has a crucial role in protecting REC8 at centromeres from cleavage by separase. During meiosis, protects centromeric cohesion complexes until metaphase II/anaphase II transition, preventing premature release of meiosis-specific REC8 cohesin complexes from anaphase I centromeres. Is thus essential for an accurate gametogenesis. May act by targeting PPP2CA to centromeres, thus leading to cohesin dephosphorylation. Essential for recruiting KIF2C to the inner centromere and for correcting defective kinetochore attachments. Involved in centromeric enrichment of AUKRB in prometaphase.
Subcellular location: Nucleus {ECO:0000250|UniProtKB:Q562F6}. Chromosome, centromere {ECO:0000269|PubMed:17205076, ECO:0000269|PubMed:18084284}. Chromosome, centromere, kinetochore {ECO:0000269|PubMed:17205076}. Note=Centromeric localization requires the presence of BUB1 and AUKRB (By similarity). During meiosis I, accumulates at centromeres during diplotene, and colocalizes differentially with the cohesin subunits RAD21 and REC8 at metaphase I centromeres (PubMed:17205076, PubMed:18084284). SGO2A and RAD21 change their relative distributions during telophase I when sister-kinetochore association is lost (PubMed:17205076). During meiosis II, it shows a striking tension-dependent redistribution within centromeres throughout chromosome congression during prometaphase II, as it does during mitosis (PubMed:17205076). {ECO:0000250|UniProtKB:Q562F6, ECO:0000269|PubMed:17205076, ECO:0000269|PubMed:18084284}.
InterPro domains:
- IPR038889: Shugoshin 1/2 (family) [1-622]
Sequence:
MEYPGIKVDTVTSGIQRRVKGRIAKTNLNVSLASKIKAKILNNSSIFKISLKHNNRALARALSKEKENSRRITTEKMQLQKEVEKLNFENTFLRLKLNTLNKKLVEIESHVSNDLLTAIEISSLSEFHQGSFLLSATKKQRNSKQCKPAHLPYARVLLTSENDDDDGADDKWQTKCNNRTISKTSPDSTSSVSRQPSSLHQCNLKAFPPKEDNQKTCGSGHLEHTSSVDILPNESHSDQSPKSSLSEMKTAPSPSLRREKLSHGNVTMRKKCVSSTPDILYVTDLDHQPTSSPGSNWNNEIHGHTNETSNNTQRNAECFLDLPSESSSEPDAKRMELVQKNTDSFHFQKTVYDAADMELTATDIGKIVAVSKSKKNQNKKKADCRKETFRKVKGASSDKKRESSKRECKDGSEVGAEEEADAARAERGAGVLDGRGDSEEPNCISSTEQPSQVNTQKKRTLQNSSDQENIQNTKRRQTYTTDEQEETNPFSRHSVKFLQDGKFDLCQKTLHHNLSKPSRQTFVIRKSEKDNLFPNQEDKDTISENLEVTNEFHIDDLSIEANENVCDHETQTMLDLKKSVSAQQNQTKINKTKQKINRRTKIISVMSQVYEDNDKDIHVLEKDNFPFHTQANKETTSGNLESSKEFESPLLFTRDNGSLRDCKTQNVLDLHKQIPDLYPDRNESQISKIPRQKVNRKTEVISGVKCFSNDQGVHCSEKDKSLLLQKDKDFPGTLKDLSEFDTPAFCNKDSAKSCDYKSEMLLGLKKHDPNMQPACQDDSKAGKKLRQKVNRKTEIISKITQIHENDRGSTHDSLNKKLCQKVNISKIISQMNQIYETINEDGNGFKSSIKDCEDIKSCDFGEINSNKKENYDPIQDPCTLVKKTKRKGSCKAGSSLAGAKNRCGLQLTDSSQVQSVPLDSGLRHHPNEADSGPGEQTNLPKMQKQSAGRSLGDAFSVSLGKEGSRPAKAVSKMTPKSKKRKLPLGCSPETHGTVEITPNTDLAKAVDSQQTEKENYLEKEKIAKRKPDFCTKVLKPLSETCSSNIKNSSLDSMCKSSLPLSISSRKTLMLEESSSLESTCIFQVGDAAHEKITTGTRNPHHRTQKSTPGSRTSLVLVDTSSVSDTNPANPENESEGQSSHPMRRKRQCVPLNLTEPSLRSKMRR
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0022414 (reproductive process), GO:0000003 (reproduction), GO:0009987 (cellular process), GO:0051321 (meiotic cell cycle), GO:0022402 (cell cycle process), GO:0007049 (cell cycle), GO:0071840 (cellular component organization or biogenesis), GO:1903046 (meiotic cell cycle process), GO:0061982 (meiosis I cell cycle process), GO:0007059 (chromosome segregation), GO:0140013 (meiotic nuclear division), GO:0070192 (chromosome organization involved in meiotic cell cycle), GO:0016043 (cellular component organization), GO:0045132 (meiotic chromosome segregation), GO:0007062 (sister chromatid cohesion), GO:0045143 (homologous chromosome segregation), GO:0098813 (nuclear chromosome segregation), GO:0007129 (homologous chromosome pairing at meiosis), GO:0006996 (organelle organization), GO:0007127 (meiosis I), GO:0048285 (organelle fission), GO:0051276 (chromosome organization), GO:0000280 (nuclear division)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0098687 (chromosomal region), GO:0099080 (supramolecular complex), GO:0043229 (intracellular organelle), GO:0000775 (chromosome, centromeric region), GO:0000776 (kinetochore), GO:0043228 (non-membrane-bounded organelle), GO:0000779 (condensed chromosome, centromeric region), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005694 (chromosome), GO:0000793 (condensed chromosome)