Protein: Peroxisomal targeting signal 1 receptor (PTS1 receptor) (PTS1R) (PTS1-BP) (Peroxin-5) (Peroxisomal C-terminal targeting signal import receptor) (Peroxisome receptor 1) (P50542)
Organism: Homo sapiens (Human)
Function: Receptor that mediates peroxisomal import of proteins containing a C-terminal PTS1-type tripeptide peroxisomal targeting signal (SKL-type). Binds to cargo proteins containing a PTS1 peroxisomal targeting signal in the cytosol, and translocates them into the peroxisome matrix by passing through the PEX13-PEX14 docking complex along with cargo proteins. PEX5 receptor is then retrotranslocated into the cytosol, leading to release of bound cargo in the peroxisome matrix, and reset for a subsequent peroxisome import cycle.
Subcellular location: Cytoplasm, cytosol {ECO:0000269|PubMed:11336669, ECO:0000269|PubMed:16314507, ECO:0000269|PubMed:7719337}. Peroxisome matrix {ECO:0000269|PubMed:11336669, ECO:0000269|PubMed:11546814, ECO:0000269|PubMed:16314507, ECO:0000269|PubMed:24662292, ECO:0000269|PubMed:26344566, ECO:0000269|PubMed:33389129, ECO:0000269|PubMed:7706321, ECO:0000269|PubMed:7719337}. Note=Cycles between the cytosol and the peroxisome matrix (PubMed:11336669, PubMed:16314507). Following binding to cargo proteins containing a PTS1 peroxisomal targeting signal in the cytosol, recruited to the docking complex, composed of PEX13 and PEX14, leading to translocation into the peroxisome matrix along with cargo proteins (By similarity). Export and recycling to the cytosol is initiated by binding to the PEX2-PEX10-PEX12 ligase complex via its unstructured N-terminus that inserts into the ligase pore and emerges in the cytosol (By similarity). Cys-11 of PEX5 is then monoubiquitinated, promoting its extraction from peroxisomal membrane by the PEX1-PEX6 AAA ATPase complex (PubMed:16314507, PubMed:19208625, PubMed:24118911, PubMed:29884772). Extraction is accompanied by unfolding of the TPR repeats and release of bound cargo in the peroxisome matrix (By similarity). The TPR repeats refold in the cytosol and ubiquitination is removed by deubiquitinating enzymes, resetting PEX5 for a subsequent import cycle (By similarity). {ECO:0000250|UniProtKB:A0A1L8FDW4, ECO:0000269|PubMed:11336669, ECO:0000269|PubMed:16314507, ECO:0000269|PubMed:19208625, ECO:0000269|PubMed:24118911, ECO:0000269|PubMed:29884772}.
InterPro domains:
- IPR011990: Tetratricopeptide-like helical domain superfamily (homologous_superfamily) [313-639]
- IPR024111: PEX5/PEX5L (family) [1-634]
- IPR019734: Tetratricopeptide repeat (repeat) [369-589]
Sequence:
MAMRELVEAECGGANPLMKLAGHFTQDKALRQEGLRPGPWPPGAPASEAASKPLGVASEDELVAEFLQDQNAPLVSRAPQTFKMDDLLAEMQQIEQSNFRQAPQRAPGVADLALSENWAQEFLAAGDAVDVTQDYNETDWSQEFISEVTDPLSVSPARWAEEYLEQSEEKLWLGEPEGTATDRWYDEYHPEEDLQHTASDFVAKVDDPKLANSEFLKFVRQIGEGQVSLESGAGSGRAQAEQWAAEFIQQQGTSDAWVDQFTRPVNTSALDMEFERAKSAIESDVDFWDKLQAELEEMAKRDAEAHPWLSDYDDLTSATYDKGYQFEEENPLRDHPQPFEEGLRRLQEGDLPNAVLLFEAAVQQDPKHMEAWQYLGTTQAENEQELLAISALRRCLELKPDNQTALMALAVSFTNESLQRQACETLRDWLRYTPAYAHLVTPAEEGAGGAGLGPSKRILGSLLSDSLFLEVKELFLAAVRLDPTSIDPDVQCGLGVLFNLSGEYDKAVDCFTAALSVRPNDYLLWNKLGATLANGNQSEEAVAAYRRALELQPGYIRSRYNLGISCINLGAHREAVEHFLEALNMQRKSRGPRGEGGAMSENIWSTLRLALSMLGQSDAYGAADARDLSTLLTMFGLPQ
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0140104 (molecular carrier activity), GO:0033218 (amide binding), GO:0140597 (protein carrier chaperone), GO:0005515 (protein binding), GO:0042277 (peptide binding), GO:0019899 (enzyme binding), GO:0005048 (signal sequence binding), GO:0051020 (GTPase binding), GO:0005048 (signal sequence binding), GO:0031267 (small GTPase binding)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0051179 (localization), GO:0009987 (cellular process), GO:0051641 (cellular localization), GO:0055085 (transmembrane transport), GO:0051234 (establishment of localization), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0009056 (catabolic process), GO:0033036 (macromolecule localization), GO:0044237 (cellular metabolic process), GO:0044281 (small molecule metabolic process), GO:0044238 (primary metabolic process), GO:0044282 (small molecule catabolic process), GO:0044248 (cellular catabolic process), GO:0044255 (cellular lipid metabolic process), GO:0051649 (establishment of localization in cell), GO:0045184 (establishment of protein localization), GO:0006629 (lipid metabolic process), GO:0046907 (intracellular transport), GO:0071806 (protein transmembrane transport), GO:0006810 (transport), GO:0070727 (cellular macromolecule localization), GO:0016043 (cellular component organization), GO:1901575 (organic substance catabolic process), GO:0071705 (nitrogen compound transport), GO:0043436 (oxoacid metabolic process), GO:0072594 (establishment of protein localization to organelle), GO:0071702 (organic substance transport), GO:0065002 (intracellular protein transmembrane transport), GO:0044242 (cellular lipid catabolic process), GO:0044743 (protein transmembrane import into intracellular organelle), GO:0043574 (peroxisomal transport), GO:0016042 (lipid catabolic process), GO:0006631 (fatty acid metabolic process), GO:0016054 (organic acid catabolic process), GO:0006886 (intracellular protein transport), GO:0015031 (protein transport), GO:0030258 (lipid modification), GO:0008104 (protein localization), GO:0006996 (organelle organization), GO:0033365 (protein localization to organelle), GO:0007031 (peroxisome organization), GO:0046395 (carboxylic acid catabolic process), GO:0016558 (protein import into peroxisome matrix), GO:0019752 (carboxylic acid metabolic process), GO:0006625 (protein targeting to peroxisome), GO:0006605 (protein targeting), GO:0019395 (fatty acid oxidation), GO:0034440 (lipid oxidation), GO:0072663 (establishment of protein localization to peroxisome), GO:0015919 (peroxisomal membrane transport), GO:0000038 (very long-chain fatty acid metabolic process), GO:0009062 (fatty acid catabolic process), GO:0072662 (protein localization to peroxisome), GO:0006635 (fatty acid beta-oxidation), GO:0032787 (monocarboxylic acid metabolic process), GO:0072329 (monocarboxylic acid catabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0005794 (Golgi apparatus), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)