Protein: Endoglucanase (EC 3.2.1.4) (CaCel) (Cellulase) (Endo-beta-1,4-glucanase) (D3GDK4)
Organism: Cryptopygus antarcticus (Antarctic springtail)
Function: Hydrolyzes carboxymethylcellulose (CMC). Hydrolyzes also lichenan and barley beta-1,4-D-glucan. CMC is hydrolyzed majorily to cellobiose (G2), cellotriose (G3) and cellotetraose (G4). Cellohexaose (G6) is hydrolyzed to G4 and G2 with traces of G3. Cellopentaose (G5) is completely hydrolyzed to G2 and G3, and G4 is partially hydrolyzed to G2. Does not hydrolyze G2 or G3. Does not hydrolyze crystalline cellulose, soluble starch, xylan, mannan or laminarin.
Subcellular location: Secreted {ECO:0000269|PubMed:24848382}.
InterPro domains:
- IPR052288: Glycosyl Hydrolase 45 Enzymes (family) [4-222]
- IPR000334: Glycoside hydrolase, family 45 (domain) [21-219]
- IPR036908: RlpA-like domain superfamily (homologous_superfamily) [17-224]
Sequence:
MKVFVVLAAIVAIANGLTSGSGVTTRYWDCCKPSCSWGGKASVTKPVRTCKANGNTTIDSNTQSGCNGGSSYVCNDQQPFTQGNVGYGFAAASISGQPESQTCCACYEMTFTNTAISGQKMIVQVTNTGSDLNGNHFDLMIPGGGVGIFNGCQSQWGAPSNGWGQRYGGISSQSECNQLPTSLRAGCNWRFGWFKNADNPSMKFTQVRCPTILTQKSQCVRTPGP
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016787 (hydrolase activity), GO:0016798 (hydrolase activity, acting on glycosyl bonds), GO:0004553 (hydrolase activity, hydrolyzing O-glycosyl compounds), GO:0008810 (cellulase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071554 (cell wall organization or biogenesis), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0044036 (cell wall macromolecule metabolic process), GO:0044085 (cellular component biogenesis), GO:1901576 (organic substance biosynthetic process), GO:0042546 (cell wall biogenesis), GO:0044249 (cellular biosynthetic process), GO:0005975 (carbohydrate metabolic process), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0044262 (cellular carbohydrate metabolic process), GO:0009059 (macromolecule biosynthetic process), GO:0070589 (cellular component macromolecule biosynthetic process), GO:0016051 (carbohydrate biosynthetic process), GO:0005976 (polysaccharide metabolic process), GO:0034637 (cellular carbohydrate biosynthetic process), GO:0010383 (cell wall polysaccharide metabolic process), GO:0034645 (cellular macromolecule biosynthetic process), GO:0044038 (cell wall macromolecule biosynthetic process), GO:0044264 (cellular polysaccharide metabolic process), GO:0033692 (cellular polysaccharide biosynthetic process), GO:0044042 (glucan metabolic process), GO:0051278 (fungal-type cell wall polysaccharide biosynthetic process), GO:0000271 (polysaccharide biosynthetic process), GO:0070592 (cell wall polysaccharide biosynthetic process), GO:0010410 (hemicellulose metabolic process), GO:0006073 (cellular glucan metabolic process), GO:0009250 (glucan biosynthetic process), GO:0010411 (xyloglucan metabolic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005576 (extracellular region)