Protein: UDP-D-xylose:L-fucose alpha-1,3-D-xylosyltransferase MGP4 (EC 2.4.2.-) (Protein MALE GAMETOPHYTE DEFECTIVE 4) (Rhamnogalacturonan xylosyltransferase MGP4) (Q9M146)
Organism: Arabidopsis thaliana (Mouse-ear cress)
Function: Catalyzes the transfer of D-xylose from UDP-alpha-D-xylose onto L-fucose. Probably involved in the biosynthesis of rhamnogalacturonan II (RG-II) through xylosylation of the internal fucose moiety of the A-chain of RG-II, a structurally complex pectic polysaccharide of the primary cell wall. RG-II is essential for the cell wall integrity of rapidly growing tissues such as roots and pollen tube growth and elongation.
Subcellular location: Golgi apparatus membrane {ECO:0000305|PubMed:21288267, ECO:0000305|PubMed:21421394}; Single-pass type II membrane protein {ECO:0000305|PubMed:21288267, ECO:0000305|PubMed:21421394}.
InterPro domains:
- IPR029044: Nucleotide-diphospho-sugar transferases (homologous_superfamily) [94-331]
- IPR005069: Nucleotide-diphospho-sugar transferase (domain) [121-339]
- IPR052636: UDP-D-xylose:L-fucose alpha-1,3-D-xylosyltransferase (family) [40-354]
Sequence:
MAQQKFLHQRPIQNPFTNPFSSSPLSTSSISNRPISLLSRNGLLLLLALLVILGVFLPWAGSPLFPSPNKLSPSQSKWRDYSLPQAVKFVAKNGTVIVCAVSYPYLPFLNNWLISVSRQKHQDQVLVIAEDYATLYKVNEKWPGHAVLIPPALDSQTAHKFGSQGFFNFTARRPQHLLEILELGYNVMYNDVDMVWLQDPFQYLEGKHDAYFMDDMTAIKPLDHSHDLPPPGKKGRTYICSCMIFLRPTNGAKLLMKKWIEELETQPWSRAKKANDQPGFNWALNKTANQVDMYLLSQAAFPTGGLYFKNKTWVKETKGKHAIIHNNYIVGFEKKIKRFRDFNLWLVDDHASESPLGKLE
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0016740 (transferase activity), GO:0016757 (glycosyltransferase activity), GO:0016763 (pentosyltransferase activity), GO:0008194 (UDP-glycosyltransferase activity), GO:0035252 (UDP-xylosyltransferase activity), GO:0042285 (xylosyltransferase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0009987 (cellular process), GO:0009058 (biosynthetic process), GO:0044237 (cellular metabolic process), GO:0071554 (cell wall organization or biogenesis), GO:0071840 (cellular component organization or biogenesis), GO:0071704 (organic substance metabolic process), GO:0044238 (primary metabolic process), GO:0044036 (cell wall macromolecule metabolic process), GO:0044085 (cellular component biogenesis), GO:1901576 (organic substance biosynthetic process), GO:0042546 (cell wall biogenesis), GO:0044249 (cellular biosynthetic process), GO:0016043 (cellular component organization), GO:0071669 (plant-type cell wall organization or biogenesis), GO:0005975 (carbohydrate metabolic process), GO:0071555 (cell wall organization), GO:0044260 (cellular macromolecule metabolic process), GO:0043170 (macromolecule metabolic process), GO:0044262 (cellular carbohydrate metabolic process), GO:0009059 (macromolecule biosynthetic process), GO:0009664 (plant-type cell wall organization), GO:0070589 (cellular component macromolecule biosynthetic process), GO:0016051 (carbohydrate biosynthetic process), GO:0005976 (polysaccharide metabolic process), GO:0034637 (cellular carbohydrate biosynthetic process), GO:0010383 (cell wall polysaccharide metabolic process), GO:0034645 (cellular macromolecule biosynthetic process), GO:0045229 (external encapsulating structure organization), GO:0009832 (plant-type cell wall biogenesis), GO:0044038 (cell wall macromolecule biosynthetic process), GO:0044264 (cellular polysaccharide metabolic process), GO:0052546 (cell wall pectin metabolic process), GO:0033692 (cellular polysaccharide biosynthetic process), GO:0010393 (galacturonan metabolic process), GO:0000271 (polysaccharide biosynthetic process), GO:0070592 (cell wall polysaccharide biosynthetic process), GO:0045488 (pectin metabolic process), GO:0045489 (pectin biosynthetic process)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0005737 (cytoplasm), GO:0012505 (endomembrane system), GO:0005794 (Golgi apparatus), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043231 (intracellular membrane-bounded organelle)