Protein: Cilium assembly protein DZIP1L (DAZ-interacting zinc finger protein 1-like) (Zinc finger protein DZIP1) (Q9VC70)
Organism: Drosophila melanogaster (Fruit fly)
Function: Component of the DZIP1-Fam92-Cby complex which promotes ciliogenesis in sensory neurons and spermatocytes by acting downstream of Cep290 to initiate early ciliary membrane formation and thus transition zone (TZ) assembly. During spermatogenesis, also regulates distal elongation of the basal-body and their docking (anchoring) to the plasma membrane and as a consequence, regulates the initiation and proper elongation of axonemal microtubules. Within the complex, required to recruit or stabilize Rab8, Fam92 and Cby at the distal basal body of cilia to promote early ciliary membrane formation and initiate TZ assembly. Also acts with Fam92 to restrict cep290 localization to the proximal part of the TZ. May also be involved in recruitment or stabilization of Mks1 at the TZ.
Subcellular location: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole {ECO:0000269|PubMed:31821146, ECO:0000269|PubMed:33370260}. Cytoplasm, cytoskeleton, cilium basal body {ECO:0000269|PubMed:31821146, ECO:0000269|PubMed:33370260}. Note=Localizes at the transition zone (TZ) in type I sensory neurons and spermatocytes (PubMed:31821146, PubMed:33370260). In spermatocytes, localizes along the TZ membranes (PubMed:33370260). Localizes at the centriolar distal ends of early spermatocytes and in elongating spermatids, when the TZ migrates away from the basal body, localizes at the ring centriole (PubMed:31821146, PubMed:33370260). In sensory neurons, localizes between the transition fiber and TZ core components (PubMed:33370260). {ECO:0000269|PubMed:31821146, ECO:0000269|PubMed:33370260}.
InterPro domains:
- IPR013087: Zinc finger C2H2-type (domain) [144-172]
- IPR051241: DZIP and RILPL (family) [12-677]
- IPR032714: Cilium assembly protein DZIP1, N-terminal (domain) [22-132]
Sequence:
MGFKGKYPQMVRETGFKLRQYRDGPLDWRLMGSYETERILREQNFELVDKALTHLSEAPLGTVLETHILDSGIAKYFVMSQYAIQYLMCCRTYLDECVTDLKEAHTTAQEEIATLRKSLSESNNEVVQLHKRITQIEAIREVVYPCHLCTKNFISNEALNVHIGRKHRVASPPSLTSATGKEKDRDKATDVHLINTIKMELEIKQLKERLNAAERNIKERSTGSKRVSPRQEQRHVGIQSNLAEPKEKDEDSGEARQSEASERKEQLTGLAERLSNFEEWQTQLKQSNEQFIQDINKRLEGLSHALEQSKQASASTPPLEDRVATPCLEDLERILTEKVAEIGKVSAHRLEEVVYHLEEGYKEKLGALERELKQLSVQKVQPEPVQTVPVASKIPKPVVRKEETNIDRIRKQVESEFLKQKHDDDTYSIEEAPRKGSEKPFPQLVTQVQVVEKEQPSAGSSDSNPTYTKSPREPAPNKQETKEATDVSDSLSQEETENEEERSLTEEEGTDVPTSGSEAAREDPTPKTIKPSGRIIKSPQKPLTRKDARKMVNRKLMSHGFDMKSKGISHNSLKRVNSELTEHRNKLKLQHPHFYATRNRIRKFVEKLCSAKFSERAEMLLKHKSPLKPMEVPGKGIPRSAISEKSEEDIASSQGEEQTDEQTDSSEQQTRSPSPQRLVSRDFKARLEEILVKPAATIRGASKSSLSSRPVPLPRKRVMFNTTEDGKSFNDSDDNLK
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0005488 (binding), GO:0005515 (protein binding)
Biological Process (BP): GO:0008150 (biological process), GO:0009987 (cellular process), GO:0065007 (biological regulation), GO:0050789 (regulation of biological process), GO:0048519 (negative regulation of biological process), GO:0071840 (cellular component organization or biogenesis), GO:0009892 (negative regulation of metabolic process), GO:0019222 (regulation of metabolic process), GO:0060255 (regulation of macromolecule metabolic process), GO:0016043 (cellular component organization), GO:0010605 (negative regulation of macromolecule metabolic process), GO:0006325 (chromatin organization), GO:0010629 (negative regulation of gene expression), GO:0010468 (regulation of gene expression), GO:0040029 (epigenetic regulation of gene expression), GO:0006338 (chromatin remodeling), GO:0045814 (negative regulation of gene expression, epigenetic), GO:0031507 (heterochromatin formation), GO:0070828 (heterochromatin organization)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005815 (microtubule organizing center), GO:0005622 (intracellular anatomical structure), GO:0043226 (organelle), GO:0042995 (cell projection), GO:0036064 (ciliary basal body), GO:0005814 (centriole), GO:0120025 (plasma membrane bounded cell projection), GO:0043229 (intracellular organelle), GO:0043227 (membrane-bounded organelle), GO:0043228 (non-membrane-bounded organelle), GO:0005929 (cilium), GO:0043232 (intracellular non-membrane-bounded organelle), GO:0005856 (cytoskeleton), GO:0015630 (microtubule cytoskeleton)