Protein: Cytosolic carboxypeptidase 3 (EC 3.4.17.-) (ATP/GTP-binding protein-like 3) (Protein deglutamylase CCP3) (Q8CDP0)
Organism: Mus musculus (Mouse)
Function: Metallocarboxypeptidase that mediates deglutamylation of tubulin and non-tubulin target proteins. Catalyzes the removal of polyglutamate side chains present on the gamma-carboxyl group of glutamate residues within the C-terminal tail of tubulin protein. Specifically cleaves tubulin long-side-chains, while it is not able to remove the branching point glutamate. Also catalyzes the removal of polyglutamate residues from the carboxy-terminus of non-tubulin proteins such as MYLK. May catalyze the hydrolysis of aspartate from the carboxy-terminus of target proteins. Does not show detyrosinase or deglycylase activities from the carboxy-terminus of target proteins.
Subcellular location: Cytoplasm, cytosol {ECO:0000269|PubMed:17244818}.
InterPro domains:
- IPR050821: Cytosolic carboxypeptidase (family) [24-824]
- IPR000834: Peptidase M14, carboxypeptidase A (domain) [304-576]
- IPR040626: Cytosolic carboxypeptidase, N-terminal (domain) [177-301]
Sequence:
MSEDSEEEDYSDRSISDDDDLDEDSFMKFVSDDIHPCTLLAADSIGDPFFPRTTQILLEYQLGRWVPRLRGPRDLYGVSSSGPLSPTRWPYHCEVIDEKVQHIEWTPFVPEPVYVPTGLEIEPVYPNSKEDTVVYLAEDDHLCKAYKEPCFVYSRVGGNRTSLKQPVDNCDNTLVFEARFESGNLQKVVKVADHEYELTVRPDLFTNKHTQWYYFQVTNTQAEIVYRFTIVNFTKPASLYNRGMKPLFYSEKEAKTHNIGWQRIGDQIKYYKNNLGQDGRHFFSLTWTFQFPHSQDTCYFAHCYPYTYSNLQEYLSGINSDPVRSKFCKIRVLCHTLARNMVYVLTITTPLKTSDSKRKAVILTARVHPGETNSSWIMKGFLDYILGDSSDARLLRDTFIFKVVPMLNPDGVIVGNYRCSLAGRDLNRNYTSLLKESFPSVWYTRNMINRLMEKREVILYCDLHGHSRKQNIFMYGCDGSSRSKTKGLYLQQRIFPLMLSKNCPNIFSFSACKFNVQKSKEGTGRVVMWKMGIRNSFTLEATFCGSTLGNKRGTHFGTKDLESMGYHFCDSLLDYCDPDRSKYYQCLKELEEMEKHLSSERVSDNTDTSLVEISLDVESSSRGSDSSESNDTQTYLLKVTSQARNKKKYLKTKRERNAILANCQNNMQEVYGKEHLLQRHDESNSDGNDPRIDAPDVYVAHCFRRPLPNQGVVKIPGQRFYPGKTWSSSQRMIKSLNKDHRTCILETCKNPIQEVQSRGINIHESCFKMAKCPMNKRPSHWIEKTRIPTESHHQLKSKAKRCSSFQSKRTGTNWTDDEKRIYRDKRIAQTQEILKYLLPIVESSQNRKSTQMNNLINPIANLQQHQLIPTACINRRRYSIPWTPTRNLPFKAQRNLMTDTSEWLQSVPLGSFESLLPLCNLQKKTKHFELWGKKAKDVQLATSQWEAVPLSSNMDASIIRGNSVLQPKEFTMRSSKQRIPYLTKTSKKPSESDGLLTFQLKIHRNS
Predicted GO terms:
Molecular Function (MF): GO:0003674 (molecular function), GO:0003824 (catalytic activity), GO:0140096 (catalytic activity, acting on a protein), GO:0016787 (hydrolase activity), GO:0008233 (peptidase activity), GO:0008237 (metallopeptidase activity), GO:0008238 (exopeptidase activity), GO:0004180 (carboxypeptidase activity), GO:0008235 (metalloexopeptidase activity), GO:0004181 (metallocarboxypeptidase activity)
Biological Process (BP): GO:0008150 (biological process), GO:0008152 (metabolic process), GO:0044238 (primary metabolic process), GO:0006807 (nitrogen compound metabolic process), GO:0071704 (organic substance metabolic process), GO:0043170 (macromolecule metabolic process), GO:1901564 (organonitrogen compound metabolic process), GO:0019538 (protein metabolic process), GO:0036211 (protein modification process), GO:0043412 (macromolecule modification), GO:0018193 (peptidyl-amino acid modification), GO:0018200 (peptidyl-glutamic acid modification)
Cellular Component (CC): GO:0005575 (cellular component), GO:0110165 (cellular anatomical entity), GO:0005737 (cytoplasm), GO:0005829 (cytosol), GO:0005622 (intracellular anatomical structure)